LOCUS       DAOQBS010000001        78330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_107_length_78330_cov_15.303085, whole genome shotgun sequence.
ACCESSION   DAOQBS010000001 DAOQBS010000000
VERSION     DAOQBS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 78330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 78330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78330
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107_length_78330_cov_15.303085"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /gene="flhF"
                     /locus_tag="PKN25_00005"
     CDS             complement(<1..886)
                     /gene="flhF"
                     /locus_tag="PKN25_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924000.1"
                     /note="positive regulator of class III flagellar genes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNV89305.1"
                     /translation="MNVKRYFAPTAREALRALKEALGPDAIVLSNRAVEGGVEILALP
                     GEAVGALQAANRAAVVARAGGEVRPAAVEMPAAAARSADDADFRVSLSALAASAARNA
                     SRPVAQPAAEPATVVRAFNPPRIETADYALRSRDGVLRAAAAAQTAARPAPSGPAVFS
                     GRAARSDADEFGSARVRELQETNARLMAELTGIRGMIERQLAGFAWGETRRQAPARAA
                     MLGELLEAGFSAAIARKLVEAVHEDADRIEAREAVGAALDRLMRACASDEDLLDRGGV
                     YALVGPTGVGKTTTTAKLA"
     assembly_gap    1095..1194
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1261..3363)
                     /gene="flhA"
                     /locus_tag="PKN25_00010"
     CDS             complement(1261..3363)
                     /gene="flhA"
                     /locus_tag="PKN25_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNV89306.1"
                     /translation="MALNDTLKLRQLLAPANLRPLAAPLLILVILSMMVLPLPVFLLD
                     VFFTFNIAISVMVMLVAMYSTRPLDFSVFPTVLLVTTLLRLSLNVASTRIVLLEGHAG
                     PDAAGKVIEAFGNFLVGGNTAVGLVVFIILTVINFVVITKGAGRIAEVSARFTLDAMP
                     GKQMAIDADLNAGLVDEKEAKRRRKEIAQESDFYGAMDGASKFVRGDAVAGIIILAIN
                     IIGGLFVGIMQHDMAVGDAAHTYTLLTIGDGLVAQIPSLIVSVAAGLVVSRVGDEGDV
                     GGLVIGQLFSNPQVMVLTAAIIGVLGLIPGMPNLVFLLLAATLGGMAWMRRKRIAEEA
                     AAAAAPEAAAQVPMPAADNLEASWNDVSQVDVLGLEVGYRLIPMVDKGQDGELLKRIR
                     GLRKKFAQEVGFLSAPVHIRDNLELKPNGYRLALKGVEVGSGDAYPGQFLAINPGRVS
                     GPLAGRETTDPAFGLPAVWIDASMREQAHALGYTVVDASTVVATHLNHVILNHAAELL
                     GRQETQALLDHLGKESPKLVEDLVPKLLPLGTVQRVLQNLLEEGVNIRDMRSIIEVLA
                     ENAPRVQDPAQLTTRVREALGRAILQGLFPGNAEVQVMALEPGLERILMQAMAAGGEG
                     GAIEPGLADTLLRQTAQIAQRQEDVGLPPVLLVPAQLRLLLSRFLRRAVPSLKVVSNS
                     EVPENRTIRVTAMVGAQG"
     gene            complement(3374..4516)
                     /gene="flhB"
                     /locus_tag="PKN25_00015"
     CDS             complement(3374..4516)
                     /gene="flhB"
                     /locus_tag="PKN25_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNV89307.1"
                     /translation="MAEDSDLEKTEDPSSRRLEQAREEGQVPQSRELSTFLVTMTGAA
                     TLLLLGGWMAGRVMGLFRDGFAFDRRAAFDENVMLDMLERMLGGALLTLMPLFVALMV
                     AAVAAPMVLGGLVFAPKVLGLKFDRLDPLQGLGRMFSLHGLVEMFKAILKSVLVGGVA
                     ALVLWYEFEHLFSLMVEPLETGMADFSATIVFATLLIVLSLGLLAAIDVPFQLWQYHK
                     KLRMTKEEVKRESKEQEGDPMIKGRIRAMQREMARRRMMAEVPKADVVVTNPTHFSVA
                     LKYDAAKMGAPVVVAKGRGELALKIREIAKESEVPLLEAPPLARALYKHCELEQAIPA
                     ALYTAVAEVMAYVYQLDAWMKQGGLPPAAPTALPVPVGMDPGVPAE"
     gene            complement(4724..5191)
                     /locus_tag="PKN25_00020"
     CDS             complement(4724..5191)
                     /locus_tag="PKN25_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNV89308.1"
                     /translation="MKKRSSVLRSSIASVLCAAAFSAAAQGGGASLPVAELGAGMYRI
                     EAEVAHTAEARQTGLMHRVAMPLHRGMVFVFPEEGAHCMWMRNTHLPLSVAFIDARGK
                     ILNIERMAPRTEVNHCAAAPARFALEMNAGWFEERGIAAGALLRGIDRLPAPR"
     gene            complement(5347..6114)
                     /locus_tag="PKN25_00025"
     CDS             complement(5347..6114)
                     /locus_tag="PKN25_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV89309.1"
                     /translation="MATKNLSDRPVLRVEGLCVSYGKVEALTDASITVGEGQIVTVIG
                     PNGAGKTTMLSAIMGLLPSRGRIEFDSVAMVAPEVETMVASGMNLVPEKRELFGEMSV
                     EDNLVLGAFQRYRMGLRDHAQTMEEIFALFPRLRERRTQMAGTLSGGERQMLAVGRAL
                     MAKPKLLMLDEPSLGLAPLITREIFRIVTQLRSRGVSILLVEQNARAALQVADYAYVL
                     ETGGIAMEGPASELAGDRRVIDAYLGVGGKHQEMLAT"
     gene            complement(6114..7928)
                     /locus_tag="PKN25_00030"
     CDS             complement(6114..7928)
                     /locus_tag="PKN25_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HNV89310.1"
                     /translation="MKPALSPRLVMAAFVALLAVAPLVLSAFYVTLLNYIGLYAMVAL
                     GLVLLTGVGGLTSFGQAAFVGLGAYTTALLTTSAELPGWLAWAGGSPWLALLVGLVLT
                     VVVAFLIGKLTLRLSGHYLPLATIAWGLSLYFLFGTMGFLGGHTGLTGIPPISLFGYE
                     LRQGEEVYYLVWLFVLAGVLTTSNLLDSREGRAIRALKGGMVMAEAMGVDTARSRMVI
                     FILAALLACASGWLYAHMQRFVNPTPFGLHIGIEYLFMAVVGGAAHVWGALVGAGVIT
                     VLKQWLQDLLPKLFGTSGNFEVIFFGVLMVLVLHKARGGLWPILVSGFKRFIPVAAQR
                     RAVDRDALALPRRELPAAGSLLLEAKAVTRRFGGLLANNNMSLEVRAGEILALIGPNG
                     AGKSTMFNQVSGVDTPTSGEVRFLGESVVGKGSRTIARMGMSRTFQHVRLLPTMSVLE
                     NVAIGAHMRGTSGVLSAAWRMDRAEEARLLAEAARQIERVGLGEHMFDEAGSLALGQQ
                     RILEIARALASDPCLLLLDEPAAGLRYKEKEALGDLLRKLRGEGMGVLLVEHDMDFVM
                     GLVDRVVVMEFGEKIAEGLPEDVQRDPAVLAAYLGGED"
     gene            complement(7925..8962)
                     /locus_tag="PKN25_00035"
     CDS             complement(7925..8962)
                     /locus_tag="PKN25_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV89311.1"
                     /translation="MDFQIALILGQDGITNGAIYALLALALVLVFAVTRVIFIPQGEF
                     VAYGALTLAMIGNGVFPATAWLLLGAGVAVAALDGLGALRAGQARKLPSVLAVNIGYP
                     LAVLALLRLLPLTELGLAVQILLTMLVVVPLGPMMYRIVFQPIAEATVLVLLIVSVAS
                     HVAMVGLGLLFFGAEGYRTPPLSDASFEVGPLTVSGQTIWVIAASVVLIVALALFFGR
                     TIYGKALRATAINRNGARLMGIPPALAGKLTFLLAALIGVLSGVLISPITTIYYDSGF
                     LIGLKGFVAAIIGGLASYPLAAFGAVLVGLLESFSSFWASAYKEVLVFTLIIPVLVWR
                     SLTSHHVEEEE"
     gene            complement(9077..10225)
                     /locus_tag="PKN25_00040"
     CDS             complement(9077..10225)
                     /locus_tag="PKN25_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV89312.1"
                     /translation="MKLKKTLFVALGLAFAAGAAHAQLKVGITVSATGPAASLGIPER
                     NTVELMPKEIAGQKVTYVVLDDASDTTSAVKNIRKLVSEDKVDVVLGSTTSPNSLGMI
                     DVAAESQTPMIAWAASSRIVEPVDEKRRWVFKTPQNDSHMSTAIVSHMTSNNVKNVAF
                     IGFADAYGEGWYEQFKSVAEARGIKIVANERFTRTDTSVTGQVLKMMAAKPDAMFIAG
                     SGTPAALPQKTLKERGWSGKVYQTHGVANNDFLRVCGADCEGTLLPAGPVLVAEQLPD
                     NNPVKASAMTYIKAYEAAHGKGSVSTFGAHAWDSGVMLQAAVPAALKKAQPGTAEFRA
                     ALRDALENMKEVPGAHGVFTMGPNDHLGLDQRARVMVQIQKGAWKLVQ"
     gene            complement(10412..11122)
                     /gene="fabG"
                     /locus_tag="PKN25_00045"
     CDS             complement(10412..11122)
                     /gene="fabG"
                     /locus_tag="PKN25_00045"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNV89313.1"
                     /translation="MGFSGELAGKVAIVTGGARGIGRTITDALVASGARVHVFDMTDS
                     NESAGVLHRVNVADAQSVADAVAQLPEPATLLVNNAGITRDRSLLKMSDEEWRSVLDV
                     NLSGAFHMLRACAPGMIAAGRGRVVNITSINGLRGKFGQANYTAAKAGMIGLTKTAAR
                     ELGPKGITVNAVAPGMVMTEMARALPAEVLERALQESVMRELATPDDIAAAVVFLLSD
                     MARMITGEVIRVDAGQYV"
     gene            complement(11198..11743)
                     /locus_tag="PKN25_00050"
     CDS             complement(11198..11743)
                     /locus_tag="PKN25_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV89314.1"
                     /translation="MTTRPHPTTKQLLDQFNTEPDYSDASQDDAEALSRDRIPVRSLT
                     AADFDAVVRIDRRTTGHDRSAYYRRKFDEALGESGVRVSVVAEQDGMVAGFLMARVDF
                     GEFGRAATAAVIDTIGVDAAASGKHVGRALVSQLLANLTSLHVESVRTEVEWNRFDLL
                     RFLDRCGFRPGQQLSLSRPLV"
     gene            complement(11779..12243)
                     /locus_tag="PKN25_00055"
     CDS             complement(11779..12243)
                     /locus_tag="PKN25_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV89315.1"
                     /translation="MTANTEIKLRPLAAGDLAAVTRLDATNGGESRAGFFERRLQAEQ
                     QSPTGFFSCVAERGGEVAGFLLGHLLDGEFGGLERVAVLDAVAIDPAAQRQGLARKLV
                     GEFDRSARERGASEMRTQAQWDRPGLVEFFSAAGFRLSPRLVLERPTEYVNF"
     gene            complement(12302..12544)
                     /locus_tag="PKN25_00060"
     CDS             complement(12302..12544)
                     /locus_tag="PKN25_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNV89316.1"
                     /translation="MALLINDDCTACDACVEECPNEAITAGNPIYVIDPGKCSECVGA
                     FDEPQCKLVCPADCIKDHPDYRESQDELQAKYDSLH"
     gene            complement(12570..13418)
                     /gene="bcrD"
                     /locus_tag="PKN25_00065"
     CDS             complement(12570..13418)
                     /gene="bcrD"
                     /locus_tag="PKN25_00065"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HNV89317.1"
                     /translation="MTITAGIDIGTGAVKTVLFRVEGDKTEWLGKRNDRIRQRDPFKL
                     AEEAYHALLEEAGLKASDVDYVATTGEGESLHFHTGHFYSMTTHARGAVYLNPEARAV
                     LDIGALHGRAIRNDERGKVETYKMTSQCASGSGQFLENIARYLGIAQDEIGSLSNQAD
                     NPEVVSSICAVLAETDVINMVSRGITAPNILKGIHISMAGRLAKLLKSIGAREGVVLC
                     TGGLALDEGLLNTLNESVKEQKMNVTAFNHPDSPFAGAIGAALWGAFRHEKLARLGGQ
                     QLAAAA"
     gene            complement(13421..14737)
                     /gene="bcrA"
                     /locus_tag="PKN25_00070"
     CDS             complement(13421..14737)
                     /gene="bcrA"
                     /locus_tag="PKN25_00070"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit A"
                     /protein_id="HNV89318.1"
                     /translation="MECFVGIDLGSTTTKAVVMDDKGQVLGRGITNSRSNYDTAARVS
                     KLEAFIDARLSLIRRELEKVPAVAGRVDEILAGLTRNFRREQFVEQLGDLEQTCIANV
                     EGPRFAGKEKVIVGALTEVFRRLREEEADKLFAPTAQRKSDFFRDLAGSRFMQIGEEV
                     ARDAGIPFDTLLHMYDKSIIEVENRPPSGDMNRKFRTAMERVRGEMSSALDTAALGAP
                     IDAALDVDMVERYVVGTGYGRVRLPFPKEHIRSEILCHGLGAHLMYPKTRTVLDIGGQ
                     DTKGIQIDEKGIVVNFQMNDRCAAGTGRYLGYVADEMNMGLHELGPLAMKATKSIRIN
                     STCTVFAGAELRDRLALGDKREDILAGLHRAIMLRAMSIISRSGGITDEFTFTGGVAK
                     NEAAVKELRQLVKENYGEVKINIDPDSIYTGALGASEFARRAVVEA"
     gene            complement(14748..16046)
                     /gene="bcrB"
                     /locus_tag="PKN25_00075"
     CDS             complement(14748..16046)
                     /gene="bcrB"
                     /locus_tag="PKN25_00075"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HNV89319.1"
                     /translation="MSAATSPEVIKEPSMVKQKEMIAGNYDRLTGTKESGEKVVSTFV
                     PGNLNELIMCFDMVNNLPETNAIQNGMRKVSGGMIMDAEKAGHSEDVCTYVKADIGMM
                     GRGNIAPNGKPMPAPDMLLLSYTGCFTFMKWFELLRHEYKCPTVMLQIPYQGDGKITK
                     NMRDFVVKQLKEEVIPMFEKVSGVKFDIDRLREYLKNSAKAEDNLVWVLESAKNRPSP
                     IDAYFGGVYYIGPMFTAFRGTTDAVEYYDLVRKEIEQRIREGKGPITPEGDMKEEKYR
                     LVVEGPPNWTSFREFWKLFYDEGAVVVASSYTKVGGLYDQGFRHDPNDPLGTLADYCL
                     GCYTNNNLPQRVELLEKYMNDYQADGLLINSIKSCNSFSAGQLLMMREIEKRTGKPAA
                     FIETDLVDPRYFSHANVKNRLESYFQMVDQKRSGASLAAA"
     gene            complement(16049..17209)
                     /gene="bcrC"
                     /locus_tag="PKN25_00080"
     CDS             complement(16049..17209)
                     /gene="bcrC"
                     /locus_tag="PKN25_00080"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit C"
                     /protein_id="HNV89320.1"
                     /translation="MSTADIIARCEALYEDLDFTAARKWKEADPSRKVIAYMPVYVPR
                     EIIHAAGMLPLGIMGGGDGLEVIHGDAYYQSYICRIPRSTIELGLSKRMDFVDGMLFP
                     SICDVIRNLSGMWKLLFPGKYVRYFDVPQNYRDDVGGSYYTNELNELREGLEHLSGRK
                     ITDDALRASIAVYNENRRLVQAVYGMRSREPWKVPSSEVYLLMRAGLVLPVEEHNQML
                     SEYLAAAENVKASKRDNCRVVINGSFCEQPPLNLIKSIELAGCYIVDDDYMIVHRWLR
                     NPVSTGGDPMQNLSLAFLHESIATAAKYDDKEEDKGKYLLEQVRSNAAEGVIFAAPSF
                     CDPALLERPMLADRCSEHNVPYISFKYAENSGQMQPIREQAGTFADSIKLWS"
     gene            complement(17212..17988)
                     /locus_tag="PKN25_00085"
     CDS             complement(17212..17988)
                     /locus_tag="PKN25_00085"
                     /EC_number="4.2.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexa-1,5-dienecarbonyl-CoA hydratase"
                     /protein_id="HNV89321.1"
                     /translation="MGEAINPLKAWLERDGGLLRLRLARPKANIVDAAMIGALQQALG
                     AHLGSPALRAVLLDAEGPHFSFGASVDEHMPEQCAQMLKTLHALVRQMLDSPVPILVA
                     IRGQCLGGGLEVAAAGNLLFVAPDARLGQPEIRLGVFAPAASCLLPPRVGQSRAEELL
                     FSGRSIDGAEAFRIGLADVLADDPEAAALKWYDEHLAALSASSLRFAVRAARCDRVPA
                     IKQKLETVEAMYLEELMASHDAVEGLRAFLEKRSANWENR"
     gene            complement(17988..19121)
                     /gene="oah"
                     /locus_tag="PKN25_00090"
     CDS             complement(17988..19121)
                     /gene="oah"
                     /locus_tag="PKN25_00090"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HNV89322.1"
                     /translation="MKETTQRVIDQTAPKHLVDHNLVPETVVPGVIYEKRPARNLQGE
                     VVPGLYNAWIWLDNPKQYNSYTTDMVKGLILAFRAASCARDVATVVFTAVGDKAFCTG
                     GNTKEYAEYYAGNPQEYRQYMRLFNDMVSAILGCDKPVVCRVNGMRIGGGQEIGMAAD
                     FTVAQDLANFGQAGPKHGSAAIGGATDFLPVMIGCEQAMVSGTLCEPFSAHKAYRLGI
                     SSQIVPALKIDGKFVANPLVITDRYLDEFGRIIHGEFKTGDELAAGKELLKRGEIDLS
                     LLDEAVEKLCAKLISTFPECLTKSFEELRKPKLDAWNRNKENSRAWLALNMMNEARTG
                     FRAFNEGTKETGREIEFTDLRQALAVGTPWTPELIESLMPGAK"
     gene            complement(19174..20241)
                     /gene="had"
                     /locus_tag="PKN25_00095"
     CDS             complement(19174..20241)
                     /gene="had"
                     /locus_tag="PKN25_00095"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HNV89323.1"
                     /translation="MSTKPYRWMMVEPGRPMVRTEFEIGELAADQVVVEVAGCGVCHT
                     DLGYYYDGVRTNQPLPLALGHEISGRVVGAGSAAGQWVGRAVVVPAVMPCGTCELCTS
                     GHGTICRAQVMPGNDIQGGFASHIVVPARGLCAVDEKRLAAAGLQLADVSVVADAVTT
                     PYQAVTQAGVEPGDVAIVIGVGGVGGYAVQIANAFGASVVAIDVDPAKLELMSKYGAA
                     LTLNAREVTGRDLKKAIEAHAKANGLRTTRWKIFECSGSGAGQTNAYSLINHGATLAV
                     VGFTMDKVEVRLSNLMAFHARALGNWGCLPEYYPAALDLVLDGRINLANFVERHPLAQ
                     INEVFEAAHAHKLSRRAILTP"
     gene            20560..22278
                     /locus_tag="PKN25_00100"
     CDS             20560..22278
                     /locus_tag="PKN25_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNV89324.1"
                     /translation="MTARSVSITFAGSGGAGVMTAGNMLLDAAGRAGWYAYMTRSSGA
                     QIRGGEAAAMMRLSTSPVQSHDDQYDLLVAIDWENVGRFSAEIPMTADSLVVGDPDGG
                     EFPEAIRAKGTRSADIPFKKMAKTIEGGRPNMIALGAVAGLVGLPEDAVLGVVRDSLA
                     KKGEAARTASEASVRAGMAFAADLPPCPRLATAQGQSERLWSITGNEAAGLGAVRGGI
                     RFVAAYPITPGTEVLEWLAPNLAKLGGVLVQAEDELASINQIIGASYAGVPSLTATSG
                     PGLALMTESLGLAVASETPITVVNVMRGGPSTGIPVKSEQSDLNIALYGLHGDAPHLV
                     VAPNSLADCAFATQWAVHLADTLQTAAIVLSDQSLGQSRATISPPADPGLRAVRLMPE
                     GEAAERYRRYTNTASGVSPMAVPGMKGYQYTADGLEHNEFGTPSSGAADHSAQLDKRL
                     RKLALHDYGTHWADIEGDGDIAVLTWGSTTGPVREALERFRASGGRARLVSIRLISPV
                     RPEQLAAALAGVARVLVVEQSHGAQFHRYLRAHYDLPGSVRAFHRPGPLPIRPNEIFR
                     QLADWS"
     gene            22281..23120
                     /locus_tag="PKN25_00105"
     CDS             22281..23120
                     /locus_tag="PKN25_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNV89325.1"
                     /translation="MEATSTCPSYSARDYKSEVKPVWCPGCGDYSVLGSITKALAELS
                     IPPERVALVSGIGCSSRLPAYTNVFGFHGVHGRALPIATGIKVSRPELTVIASGGDGD
                     GFSIGGNHFMHACRRNVDMTYIVMDNEVYGMTKGQASPTTAPDWEKSKLTPQGTGINP
                     FHPLVIALAAGANFIARGFSGDQNGTAKLITEAIRHPGFSFVQLLSPCVTFRPEQRDW
                     KDMVHPAAVDPTDDPARAARRLMSDDGFNVGVLFRGSRKPFQPGQPASVDSVADLEKE
                     FVL"
     gene            23117..23656
                     /locus_tag="PKN25_00110"
     CDS             23117..23656
                     /locus_tag="PKN25_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNV89326.1"
                     /translation="MSKPDPQETGIHSVEHLLAHALAMEVEAVERYEALADQMETHNN
                     RDVAVLFRKLAEIEKLHVDNVNDISEGHTLPHIAPWEYSWEAPESPEAPSVSAEGLHY
                     MMHPYQAIAMALEAERRGVDFYDRLVAQSEREDIRKLAAELCETEREHVTLLEGWLAR
                     FQPPPKGWNEDPDPPLLQG"
     gene            complement(23910..24563)
                     /locus_tag="PKN25_00115"
     CDS             complement(23910..24563)
                     /locus_tag="PKN25_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HNV89327.1"
                     /translation="MFVENIMTREVLHVGPEASFSQVSEIMRLKKVRHVPVIDQDRKV
                     LGIISHRDVQRAQPSMITTLDVGEVKYLLSKITAADIMHKSVVSCSPRTQIEEAARMM
                     RPKKLGCLVVVDDAGRLVGIVTSVDLLDFFLDITGCWVEGATRITVKLQDRAGQLAAL
                     LASVNAQGGYIVSVVSPRTPQSEGLRTATIRFEAKDAGAVVQGLREAGYELSVDQTA"
     gene            24909..27650
                     /locus_tag="PKN25_00120"
     CDS             24909..27650
                     /locus_tag="PKN25_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV89328.1"
                     /translation="MTVSSPQGTTKVATYCYQCVAGPDLLKVKVEDGVATAVEPNFDA
                     EDVHPAAGRVCVKAFGLVQKTYNPNRVLQPMKRTNPKKGRHEDPRFVPISWDEALDTI
                     AAKLRGIRETGLLDASGYPRVAASFGGGGTPTAYMGTFPALLAAWGPVDMSFGSGQGV
                     KCYHSEHLYGELWHRAFIVCPDTPRSKYILSFGSNIEASGGVCGVWRHAAARVEQGVK
                     RVQVEPHLSVTGGCSAEWLPIKPKTDAACMHAMIHVMLFENARSRLDIDFLKHMTASP
                     YLVAPNGFYLRDPDTRKPLVWDLKSGKAVVFDTPGIDPALDGDFLASGLEVLPDQELV
                     THEAAPVRSAFGKLLDHERTFTPEWAQGICDVPAATIRRIANEYLDHAEIGATIEIEG
                     RTLPYRPVAVTLGKTVNNGWGGYDCCWARTLMACLVGALDVPGGTIGTAVRLNRPAND
                     RQSSAKPGVDGFMDYPFNPTDKENWISRPQIRNANRTLVPLVANSAWSAALGPTHLAW
                     MQQRHGFESFPEPTQPDVWFFYRTNPVISFWDTPQVAEAVAKFPFVVAFTYTRDETNH
                     FADILLPDCTDLEGLQMLRIGGTKYVEQFWDHQGFALRQPVVPTQGDTRDFTWISTEL
                     ARRAGILEPYNKAINRGAAGVPLKGPNYDFSLQPDHAYGVEEIWDASCRAASAELTEG
                     AEDHGLEWWKEHGFRTIEYPRLQWYLYPHLVDQGLRFEMPYQERIYRIGEELGRRLHE
                     SGIDWWDRQLTEYRPLPEFHDFSHLIKHAVISNLGGRDEDFPFWLLTARSMQYSWGGN
                     VSLQMVREVAANVKGHRGVIMNPTAARKLGIEDGDLVEVRSPLRETRGRVVLRQGIRP
                     DTLLMVGQFDHWITPYAKDFDVPSMNALVPMLMDLTDATGSAADIVPVSIKRIGGAQ"
     gene            27647..28297
                     /locus_tag="PKN25_00125"
     CDS             27647..28297
                     /locus_tag="PKN25_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV89329.1"
                     /translation="MTRYAMVVDLRRCVGCQTCTAACKHTNATPPGVQWRWVLDVEAG
                     EFPDVTRSFVPVGCQHCDEPPCETVCPTTATKKRADGLVTIDYDLCIGCAYCSVACPY
                     NARYKVTRDTPAYGEGKMANEVKRTDPARIGVATKCTFCSDRIDYGHAHGLVPGVDPD
                     ATPACANACIASALTFGDIDDPDSKASRLLRENEHFRMHEELGTGPGFFYLWEKKQ"
     gene            28294..29175
                     /locus_tag="PKN25_00130"
     CDS             28294..29175
                     /locus_tag="PKN25_00130"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HNV89330.1"
                     /translation="MRGVEPELQPFWDERAAGNFIGGGTGAGLLLWSAIAASGHGTAI
                     GLPLFLALAFIGVGLFCVWMEIGRPLRALNVFFHARTSWMTREAIVAGPLFLCGLIAL
                     LSGWLFFAWLAALIGLVFLYCQARILQAARGIPAWREPALLPLIVVTGLAEGAGMLAL
                     LSPLLVRSPGAEFAFPLLVLVVLRFWLSRRYAAHLARPNNAPLKTLRALRENDRHFLL
                     LGHTLPAAVLLIALFAGGSFGTALLALAGIAVAASGWLFKLALITRIAYTQGFALQHV
                     PARTPGYSHPGVKPGWN"
     gene            29241..29813
                     /locus_tag="PKN25_00135"
     CDS             29241..29813
                     /locus_tag="PKN25_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNV89331.1"
                     /translation="MYEVRIHGRGGQGAVLAAQLLATALVFDGKYAVSIPAFGFERRG
                     APVVSFIRASEQPIRQLTNIYAPDCIVCVDPALARTVNIFAGLKRGGTLVQATSRPLA
                     EIDVPDSVGTVGLCDAVRIATEIFGRPITNTLMLGAFARSTGQVSLEALKKVIEQSDF
                     RDAGLRQNLDALERGYAETTVHTLERRITA"
     gene            29810..30112
                     /locus_tag="PKN25_00140"
     CDS             29810..30112
                     /locus_tag="PKN25_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV89332.1"
                     /translation="MSERHQHYPAWHLKEAGVPDDLCPVATEQSPMLPGDWRSFRPVV
                     DRDKCVKCAVCWLYCPVQCVEEHAAWFDFDMKTCKGCGICAHECPQRAITMIPEAQ"
     gene            30109..31365
                     /locus_tag="PKN25_00145"
     CDS             30109..31365
                     /locus_tag="PKN25_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylglyoxylate dehydrogenase"
                     /protein_id="HNV89333.1"
                     /translation="MNTTTLDTAPTTEKRPAPQLMLCEGNEAAALGVALARPDMVAVY
                     PITPQTSLVEKLAKLIADGRMDADIVDAEGEHSVLSVLQGGALAGARTYTATCGPGLA
                     FMFEPYFRTSGMRLPLVMTIVTRDGITPQSVWGGHQDAMTVREAGWIQMYCETVQEVL
                     DTTVMAFRLAEHHDVMLPVNVCLDGNYLSYGTSRIEMPDQAEVDDFMGHKDVNWHVAL
                     DPLRPMAVDPLTGGSGGNGPETFVRYRRGQCAGMKNALHVITEMHEDWARRFGEAHRF
                     APLVEEYRLDDAEYAIMTLGSMTGAAKDAVDEARAAGEKVGLIKIKTFSPFPVEALQH
                     ALRGVRALGVVDRSVNFRWNCGPMFQETLGVLYRLGRQVPAQSFIGGLAGADLTVTTF
                     RRVIDETRALLERAPSDEPVWLNAKD"
     gene            31390..32646
                     /locus_tag="PKN25_00150"
     CDS             31390..32646
                     /locus_tag="PKN25_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV89334.1"
                     /translation="MHTTDYLIAGSSHAALEAINAIRMHDGDGSITVVTRDPHRPYSP
                     TVLPYVVSGRSTPERVFLRDDDFFTANKVDYRSGCALAGLDPAAHQARLADGSTIGYR
                     KLLLATGASPIVPPIPGIERVDYHVLRTLDDATRLRAAMAASKNAVVLGAGLVGMHAA
                     ENLVKAGASVTIVEMSKQLTSGYFDEVAAGMIEQAFLGNGAKIRTGHRVVGLEPSADG
                     ATLKLDNGDTIPTDLLLVAVGVRPELGYLDGSGIATERGILVDDTMRTSAEDVWSAGD
                     CAQARGFFTGTPVMNAILPDATLQGRIAGMAMAEDPGLKPYAGGLPLNTYHFFGRHAI
                     SVGSAAVPEGGRAQVRFDAASGRYLRAVFDRDERLTGIFGVNEFFDAGVMAQLILRRT
                     DLGPLRERFFAQPLATGRELMSQLWR"
     gene            32657..34012
                     /locus_tag="PKN25_00155"
     CDS             32657..34012
                     /locus_tag="PKN25_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV89335.1"
                     /translation="MGQAYSTIAFDPAKCDGCGKCMTACASVKFDSTDTTRSLIQIVR
                     SGGARIEPPRPGEFELALCRQCADPKCVTVCPAGALGKNGTTGVIDWDASKCVDCLLC
                     TIGCTYAGIALDEHSGRVSKCDTCEGKPACVPACPTGALRHITTARIYNEVGSWEDLF
                     APGLAGCQGCNTELLMRHALRRVGPDTVLATPPGCVPGMGSVGFNGTTGTKVPVFHPL
                     LTNTASMLAGVKRQYERVGRKVTAMAIAGDGGASDVGFQSLSGAAERGERILFMVVDN
                     EGYMNTGMQRSSCTPYGAWTSTTPIGAHGRGKTQDAKNLPMIMVNHRCEYVATASTAY
                     MEDLYEKLDKALAASERGFAYLHVYSPCTTGWRFQSALNIEVARKAVESNFVTLWEYT
                     PEAGLHFTRPVDKPLPVADYLKAMGRFRHLSPEQVEHIQNKVAENLRFVRKIAEQADE
                     Q"
     gene            34002..35321
                     /gene="paaF"
                     /locus_tag="PKN25_00160"
     CDS             34002..35321
                     /gene="paaF"
                     /locus_tag="PKN25_00160"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNV89336.1"
                     /translation="MSNEKPSPPEQMSRAELQALQLERMRWSLQHAWDNVPHYRHAFE
                     AAGVRPSDLRGLADLARFPFTAKQDLRDNYPYGLFAVPMAKIVRVHASSGTTGRPTVV
                     GYTKGDIERWAAVMARSLRVAGASCEDIILNSYGYGLFTGGLGAHYGGEALGATVIPM
                     GGGNTEKQIQLIQEFRPTVIMATPSYMLTLADTMQRMGLDPSATTLRLGMFGAEPWTN
                     QMRAEIERVLGIDAIDVYGLSEVMGPGVACEFVGHKDGPHIWEDHFYPEIIDPVTGEV
                     LPDGERGELVFTSLTKEAMPVIRYRTRDLTRLLPGSGCAMRRIDKITGRSDDMLIIRG
                     VNVFPTQIEEVLLRHDKLCGHYQLVLTREGHLDQLTVRAELRPELADGIGQGLRRQIG
                     EEVRHAIKSFVGISAEIEILDPDSIERTQVGKAKRVIDRRNAATSTA"
     gene            35372..37462
                     /locus_tag="PKN25_00165"
     CDS             35372..37462
                     /locus_tag="PKN25_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNV89337.1"
                     /translation="MTEVRSHAASRAAGEAAQRYRTALATGRATLDADELARLLAAAD
                     LHTTTAPADAIELSIRVHATREFGLVLSAGAGGLDGALDPANFARDRAAVHAAVELTD
                     GEDFLERFRRTIAWQRITALAARRGVQPPDAALARLFEAALQLAAGGLPDAPGAQAAL
                     QELALDCACDGEAVRVVAARCSVGAPPPLRVARPIHKIDRLLHPERIGIVGASASGMN
                     FGRIILRNLLGSGCAPERLCVIRPGGGEIDGVACIENLAAIEGKLDLLIVAVAADAVY
                     PLVDEIIAAGTVEAVMLIPGGLGETAKSRAPAADLAARINAAHACGDGGPIFLGANCL
                     GVVSHPGRYDSWFIPLERLPKPQKAPERRAVMLSQSGAFMITRLSHNPWLDPRYMIAM
                     GNQTDLTHGDLLSWFAAREDVDSIGIYVEGFRDLDGLAFARAVRRAVLAGKQVVVYKS
                     GRSEAGQAGVMGHTASIAGDPVLFASVLAQAGATVTDDVEVFDDLFYMAGTLHAKRIG
                     GDRLAAASGAGFEAVSAADAIVGETFSMRMAALSADTVGRIEAVLAEKKLAALVEVRN
                     PLDMNPGADDDAHVRITAAMIEDPEVDAVVVGLDPTAPMVRALEQSRLRPGYDLSDPE
                     STVHTLPPLVARTDKPIVAVVDAGRLYDAMAAGLMDRGVCVFRNVARGTRALVRHVEA
                     RLDAERIRRHFGQS"
     gene            37527..37934
                     /locus_tag="PKN25_00170"
     CDS             37527..37934
                     /locus_tag="PKN25_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV89338.1"
                     /translation="MSDTLQPGLSLTRKYDIDAARTIAFMGDECRVYSTPALLYDIEV
                     CCRDLLLQHIGEGKDSVGTRVELDHVGATLLGMWIEITVKLVQVDGPAVVFEFSARDA
                     LEEVARGRHNRFVVGVDKTRQRLLGKLAKAQAA"
     gene            complement(38146..39159)
                     /locus_tag="PKN25_00175"
     CDS             complement(38146..39159)
                     /locus_tag="PKN25_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNV89339.1"
                     /translation="MNAFSTPRRVRFFATRIAACCAALCASLAPAAGDTSVHGAAAAA
                     AAFVPRPAVRSAFAERSMILDATRAGPRLVAVGERGVVLLSDDDGAHWRQAGKVPVDA
                     TLTAVSFADAREGWAVGHLGVILHSADGGETWSVQRIDPVEDRPLFAVAFTDARNGVA
                     VGLWSLMLRTRDGGRTWEAAELPPPPGDTRADANLMSVFGDGQGRLFIAGERGLVLRS
                     LDGGATWSYHPTGYRGTFWSGTALADGTLIVGGLRGSVYRSTDNGEHWQRVETGSQSS
                     VTDLVAFDGRVHAVGLDGVSLDSRDGGASFAVERRADRLSLTAAVATAGGVLHFSRAG
                     LVR"
     gene            complement(39173..41566)
                     /locus_tag="PKN25_00180"
     CDS             complement(39173..41566)
                     /locus_tag="PKN25_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNV89340.1"
                     /translation="MLNPFSVSEVDTFLTRYLRLLEAVCFRFRGTILVALMLFTALMG
                     YFALQLRMDAGFEKQMPIGHEYIQTFDKYREDVLGANRLNFVVKARSGDIWTQAGLER
                     LYEVTQAVSFLPNVDRLGVQSLWTPNSFVNEITEEGFRADPLISGTIMPRDLTPEIIA
                     GIQRAASQGGFIGTLVARDQTSAMIVAELHERDREGKALDYVAYNRILEELRAKFEDA
                     QFEVQIIGFAKQIGDIADGASAVLEFCAIALLLTALAVYWYCHSVRFTLLPIACSLTS
                     LVWQFGTLRLLGYGLDPLAVLVPFLVFAIGVSHGVQQINFIVRELSHGKSSDEAARAS
                     FTGLLIPGTLALVTAFVSFITLILIPIPMVRELAITASLGVGYKIVTNLVMLPLAASL
                     FTFTKEYADKAVLKREQRSAWLRTLARVAEPRNAAIVILATLAVFAVAVWQSHDRVIG
                     TVQPGAPELRAEARFNRDAVSIASSYDTGLDWLTVIFEAPPDACDDVAVGRYQDRFVA
                     EIQYVPGVLSALSFSGQLRTYNEGYNEGNPKMSVVPGDPGNYAALATEIGRVRGFMNK
                     DCSMTAVHLFLSDHKATTINRVIAAVKAFRERDREPGLTIRLASGNAGVQAAVNEEVH
                     KSELPMMLYVYAAIMVLVALMYRDFRAVVACCLPLTVGTFIGYWFMKELEIGLTVATL
                     PVMVLAVGIGVDYAYYIYNRLQLHLARGENIVKALEHSILEVGTATIFTAITLAAGVA
                     TWMFSELKFQADMGKLLAFMFMVNMVMAMTALPAFAVWLERLFPRKGPVRAPGILAH"
     gene            complement(41642..43369)
                     /locus_tag="PKN25_00185"
     CDS             complement(41642..43369)
                     /locus_tag="PKN25_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HNV89341.1"
                     /translation="MTTHLKKLVLGLAMAGICAPAAYAFQFEAGSVRGSLDSTLTAGF
                     GKRVEGRECSHHGDQSTPCGADANTAVWGNGDDGNLNYDKGDLFTTHLKGSHELLLSF
                     PDEWKFMGRVGWLYDFVADDTARTPLDGDAKQAVGQYVRLYDLWVSKEFDLGERRGRV
                     RFGNQVVSWGESLFMIGGINSNVAMDLQRLSSPGVQLKEAFLPSPALSVAANLGSGVN
                     VEAYYQFQWKPYSFPPAGTYFSQSDFFDKGRDSLIYFTPDAASRIRTPGDPLYGKTLA
                     AAREEMLNSELTAIPVDADDEPGDSGQFGVSLKYRPEGMDVDLGLYYQRFHDKTPNLQ
                     YWNANGAARMYFLEDRELYGISANTSVGNWAVGAELSYRPEDAVSLGACQDIGFGSPD
                     QCDGSIDEERYQFHLTGILSLTPGDHGWFLDLMGAQTGTFLGEAVAIAYPGVSKDKAY
                     VRTRNGVTYQQLPAAGAWTHDSGVGDKLSWGYMFDFSLTYDSTLIPGWQVIPGVFFSH
                     AVNGNTPNFMANWMEGAKSANFYVLFNRNPMTWQAGINYTRFWGGDYSFSAPYKDRDF
                     VGGFISRNF"
     gene            complement(43462..44829)
                     /locus_tag="PKN25_00190"
     CDS             complement(43462..44829)
                     /locus_tag="PKN25_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HNV89342.1"
                     /translation="MRFCKLTALAGALLASHAALAADAASLGTTLTPLGGERAGNADG
                     SIPAWTEGSALEPGWSYGKPRADYFKHKGDKPLFTIDAANVDKYAGKLTEGQVAVIKG
                     FKNYKLEVYPSRRYCSAPDFVQANTKANVGMAKIGADGWSLAEATVPGIPFPLPQSGI
                     EVLWNAKMKYAGVALDMKALWVMLSPRSGGSDWIEAGSTQTYYYPWGKKGSNKLSELP
                     PVEYHTYFNYTSPTALAGQALVITSYLNKTSDTFYYFPGQRRVRRMPSYSYDAPQVGF
                     ENQYTLDEPRVFNGTPDRFDWKLVGKKEMFIGYGSFGMYDPAADRRKVVTPDGVDPKA
                     TRYELHRVWVVEATAKDGVRHVAPKRRFYFDEDSWALMGAEDYDAQGKLWKVRESFLI
                     PVAETGACDNPAFVQYDLVSGRVLFDQAGMGAGKDMVWAVEADDPKYKDAFYTPDNLR
                     AISDR"
     gene            complement(45053..45427)
                     /locus_tag="PKN25_00195"
     CDS             complement(45053..45427)
                     /locus_tag="PKN25_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV89343.1"
                     /translation="MSRSVPLRAVVAILGVLAVPAPALAADIGESGFVQCAACHPAAA
                     GQPHGVGPNLHGRFGRAIAGTPGFVFSRALRKREGVWTEAELDRWLADPQAYAPGTKM
                     AYAGLKDSVQRRAVIEYLKTLR"
     gene            complement(45442..46884)
                     /locus_tag="PKN25_00200"
     CDS             complement(45442..46884)
                     /locus_tag="PKN25_00200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV89344.1"
                     /translation="MEYKTSAASAAGADDSARRRFLKLASTAAAASVLPVGVGAAAPA
                     QPGYDVVVVGGGFAGVTAARELAHRGARVLLLEARNRLGGRTFHANFGERKVELGGTW
                     IHYTQPHVWAEVMRYGMEIAETPGVAHPDRVLWMSEGKVLEIPVMENWALLEDALKRF
                     HAEAGEVFERPFMAGLSAAGRKLDHLSIADRMAAMDMSPAQRDLMNAMMATNCHGPIA
                     SGAYTEMLRWWSLVDGDAARLLYSCARYKLKDGTSTLIERMAEDGGFDVRLSTAVAEL
                     SQDAAGVSLVTEADERISARFAVVAVPVNTAGQIEFSPPLRPGKTAMAKEHHAGKGHK
                     LYLKVKGRLETLIFFAPETELFTMVFTDQPGEDGGVLVAFGPPVDGRVDLNDPKAVEP
                     FVRRFLPHLAVEQVLGYDWGLDPYSRGTWCTLRPGQFGKHLEDLRAREGRVFFAGADI
                     ATGWRGFIDGAIESGLQVGQAVAAELRGRA"
     gene            complement(47050..48063)
                     /locus_tag="PKN25_00205"
     CDS             complement(47050..48063)
                     /locus_tag="PKN25_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV89345.1"
                     /translation="MPPAAPDAAPVSAGLPGPVQVGDVSDFGRGLLAWNATYEQLAPG
                     CFEGGAEELWIDDGLEVLWETGNRSVWTAGSNRAGMVSLGIPVAGGGAGMYCGVPIQD
                     GAVSWLPGGGDFEIFSRERMDIVSATVSESLLCGFAASDSPRTAEASLQRPFLQQQPR
                     RAGMLRRALIEIVVVARRQPGLLEIPASRAAMRDCVLSLMLDTLDRASERPTADLRPS
                     VKAWIVRKVRELALERPSEPLQIADICRSLAVSRRSLQYAFEDLVGMGAVEFLRNVRL
                     NAVRRELRVATGSPDEPIAAIAARWGFWHMPRFAAYYRALFGELPSETRRREGGSTPH
                     GPG"
     gene            48286..48762
                     /locus_tag="PKN25_00210"
     CDS             48286..48762
                     /locus_tag="PKN25_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNV89346.1"
                     /translation="MGRGNDETSAQTPPEGIRFGMLPELIGYRLRIAQIGIFRDFCTS
                     MDEAEITPTLFGVLVLIDANPGMKQTQLADAIQLDRSSVVSVLDKLEARGLVERVRAP
                     DNRRANALHLTATGRHLLQRLIPQVRTHEERVLQDLDPAERVQLRKLLGRLLLAAG"
     gene            complement(48786..49820)
                     /locus_tag="PKN25_00215"
     CDS             complement(48786..49820)
                     /locus_tag="PKN25_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HNV89347.1"
                     /translation="MTRTPKVCFDRVLPRDLMRFQTTMPDRPGQVRGLSPIGKTWING
                     STLHVRFMGGSAQQKKKVQEQAAWWTAHANLQFVFDDAPAAEIRITFDENDGAWSYIG
                     TDAKSIPVNEPTMNLGFLDGGTAAHEFGHAIGLAHEHQNPAGGIQWNEQAVIRAMAGP
                     PNHWSETQTRHNILRKYAANQVNGTDFDPKSVMLYFFPAEWTLNGVATSRNEVLSELD
                     KQFIAGAKMYPRAKPGVTGEVTELKVNARRRTAASIGKFGEEDVFRFRVTQPGRYVVD
                     TRGPTDVYMKLFGPDSPTALIAEDDDSGLGSNARIATDLVEGEYFVQVRHWNRASGVG
                     DYTVAVRTAN"
     gene            complement(49956..51086)
                     /gene="peaD"
                     /locus_tag="PKN25_00220"
     CDS             complement(49956..51086)
                     /gene="peaD"
                     /locus_tag="PKN25_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009310 - amine catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     beta"
                     /protein_id="HNV89348.1"
                     /translation="MSLIPDLRALRAAGAIAAAALATACATPAQKSATSDAEYLLTVT
                     RPGLLHVIDMKTDRIARSCELPGKFGSGALVPTRDGRHAFVLGNLWEDVYGFELETCK
                     LVFSARQSEGDVTVKTVQSLAVSADGSELYTVQNPVRRGADRFEVLPPRLAVYRIADG
                     LDAKPVRSFPVKRRITKIAATAGGEVILGGADVEAIDVRSGAVRTVSALASWDRGPRW
                     LTPDAFAMHSQGEHLNEYVMPYVTAKFADDRQDMASAEWWWGMSRVDLATGKAEAREI
                     FPFEFVIFSLVADPRDRNILYGAFNTLSRHDIEKRETLKVVELDHTYYSLQLSRDASR
                     VYVGGAGSTISIHDSGTLEKIGTIQLPGDMSTADVRVAWRRP"
     gene            complement(51102..51428)
                     /gene="qhpC"
                     /locus_tag="PKN25_00225"
     CDS             complement(51102..51428)
                     /gene="qhpC"
                     /locus_tag="PKN25_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     gamma"
                     /protein_id="HNV89349.1"
                     /translation="MKHLKSLNKKAQRLDAAEGKPEAIEEVVAMQTVVGCTATTDPGW
                     EIDAFGGAASLCQPMEADLYGCADSCWWPAQVPDTMSHYPDWGDGKADATRDWRKLDG
                     IFEDKI"
     gene            complement(51425..52858)
                     /gene="peaB"
                     /locus_tag="PKN25_00230"
     CDS             complement(51425..52858)
                     /gene="peaB"
                     /locus_tag="PKN25_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase maturation
                     protein"
                     /protein_id="HNV89350.1"
                     /translation="MTTLTLIRPNLHRVDSAGRQLLFHVPSSSLFELDGAARDVLALF
                     DEQATLETATLADRLGGRHDALALRETVDELRQLHVLAEHGEDGPIEPPPDPPTRFPL
                     DTLVLNVNTGCNLSCTYCYKEDLSAPAKGLRMSLETATKAIEMMLAESPGKPKYTLVF
                     FGGEPLSNMGLIRAVVDFAERRFAEVGARVDFTLTTNATLLTAEIIDWLDAHRVGVTV
                     SMDGPPAVHDRNRRSFGGEGTYDVVRRKVDLLLARYRARPVGCRVTLTRGITGVVPIF
                     EHLHHEVGFAEVGFGPVTSGDIQAFNLTADELAEVFDGLKALGRRYLDAALRNRSLGF
                     GNLHQLMTDLHEGNKKRLPCGAGVGLLAVDGKGGLNLCHRFTGSALPTFGDVDAGIDR
                     ARLGAFIGRRLDRNGTGCATCRIRNLCSGGCYHESYAKYGDAAAPSYHYCELLRDWID
                     FGIETYARIVTENPGFFAAHVSPRRTA"
     gene            complement(52875..54467)
                     /gene="peaA"
                     /locus_tag="PKN25_00235"
     CDS             complement(52875..54467)
                     /gene="peaA"
                     /locus_tag="PKN25_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009310 - amine catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     alpha"
                     /protein_id="HNV89351.1"
                     /translation="MEKERNITSMIAAGLLALAAVVGAPERALAADGAALLAANCLSC
                     HSAQGGSISRVEGQRKTPEGWQMTITRMQEQHGAKVSAEDKRRLIKYLADTRGLAPAE
                     TAGWRYLLEHDNNRVETIDGRYRDMCARCHSGARFALQRRSEDEWKLLMHTHIGLNPT
                     LEFHSLARDRQWFPLAVNEVAPALARDFALDARAWKAWQAAPRTALDGSWRIAGFLPG
                     KGAFEGRMRASAAGEDRYELAIEGRYADGALLSGRGVATVYTGYEWRGSVDIDGVALR
                     QVMAADARGERMHGRQHFADSRRMGGALSALREGAAPMVMAVMPAQLRRGETAELTVV
                     GSGLAGAVSLGKGVRVLEVVSRSADRVVVRAHAEGRDGLNDVAVGSARGKGLLAVYGR
                     VARVEVEPANAIARVGGPGDAQMQKVGVAYRAIGWAAGRDGKAGTADDLRLGYLPARW
                     SIEAADEEAEKAHDADFVGHIGADGVFVPGDAGPNPARPKSLGNTGRVNVVATVEAAG
                     ATVEGRGKLDVAVPDFVQRALD"
     gene            54720..55448
                     /gene="torR"
                     /locus_tag="PKN25_00240"
     CDS             54720..55448
                     /gene="torR"
                     /locus_tag="PKN25_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HNV89352.1"
                     /translation="MSKETPAMPHIMVVEDDPVSRALLVGYFEKEGYRVSASDNADDL
                     LQRVEQERIALVLLDIRLPGKDGLTLTRELRAASRVGIILVTARDDEIDRIVGLELGA
                     DDYVTKPFNPRELLVRARNLLWRVAQCAGSETREAEQGCFRFEGWTLNPHKRQLVSPD
                     GRIERLPQGEFKLLMALLSHAGQVLSRDQLMDAIHDREWTPNDRTVDVLVGRVRRKLD
                     DNPADPRLILTAHGAGYLFAGELD"
     gene            55468..61797
                     /locus_tag="PKN25_00245"
     CDS             55468..61797
                     /locus_tag="PKN25_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV89353.1"
                     /translation="MNAPPLQYRLLKRLYQGRCSTVSRVLLPGRDAPVIVKQLDRGRC
                     APGAPARLQHEFELLGTLELEGIPQALEQLEFERGPALVFADREAHSLRQAMRPGSRD
                     WHDWLALMIRVCELLGRLHEARVIHKQINPDHILVTPSGAPLLVDFGMATRFLREQAD
                     WNTPQLAGHRLPYLAPEQSGRINREIDYRTDYYSFGATLYELLTGQPPFAGREGLELV
                     HCHIAREPRPPHHINRLLPEAVSAVVLKLLAKDASERYQSAHGIVHDLRECLQRREQP
                     AAHDFVLASRDVSARFQVPRRLYGRTAQLAELEGRVEACAAGGRAIVLISGYTGVGKS
                     SLVHELRRAVLERHGHFASGKFDQYRRNPPHSALLQALRELVRQHLTEPGERLAALGS
                     RLREQLGGYLGTLVRLLPELGLVVPDAGTSPAGQHRFDEQGRLHLFSRLIDALTEPGQ
                     ALTLFLDDLQWADPASLGLIESLATHAGLPRLLLVGSYRHNEIGPGHALAGFVEHLRR
                     SALPPVELRLQPLDLAQVRQLLADTLRCSPIECARLAEACHEKTQGNPFFLNQLLDAV
                     HEEGLIRFHGQRWEWDEAAIRARALSTDVVELMVERLRRLPERTRAVLPLAACIGGTF
                     DLKTLSIVAEGRARQLAHDLVPALGEGLVVPLDDGDRPERDRLGRARYRFAHDRVQQT
                     AYSLLADDAREQLHLRVGRLLRQNLDTGALDRRVFEIAGHLNLARSAIDDPDERIALA
                     TLNLCAGLRARESAAFESALDYLDSGLHALPADAWRTQHRLCLDLHLAAADAANSRGD
                     GERMQALLDAAQEHAHAPLDRVRCEQIRIQAHVAHNRFGAGLEIARDTLARLGVHLPA
                     APGRLRIAAELLRTRLLIRHTAPEELLHMPAATEPRVLAAQALLGGMFGIVKFSSSAL
                     RPLVMARQVELALRHGHTDASAQALAGFGGVLCGVLGAIDEGYRAGTLALALEAARPS
                     RQGRQRTLSLFNCYVRHYREPLQCALDGLREAHAEALACGDLEYAAYSLAAAIQYAVP
                     LADDLEALARRMRGQLVQLEQTGQQQSLQYSYMALQAVTRLCEPSASPTALDGEHYRE
                     AAMLEAHRRGNHLTAVCLHHFYKALLALVHADYPAALAECTLGEAHLPYVGGTYTATW
                     FRSLHALALLRTLPPPGKARRRALHTLRRLVRHVRMQARHAPANHAHRVALIEAERLR
                     VRVRPGAAAAYDRAIALAEQGGFWLDAALACEFAARFHLDAGERAVARSCFDDACRRY
                     QRLGAHSPVVHLHEQLEEYGLWPRPPGLAGAGEMQGGLDETNHSFDLSSVIEASQAIS
                     DEIVLERLIERLLQLALTSAGAQRGALALSRDRHLRVEAIAGLEEGSTLMCSLALEAA
                     TSRVPVSVLNYVARTGEALVLGDAPRQQMFAHDPYIRSQAPRSLLCMPILYHGELTAL
                     LYLEHRLGRDIFDQQRLKTLRILAAQAAISIENAKLYLGLQQSEQAYRSLYENAIEGI
                     FRVDPDGRFLSANPALVRLLGHDSAAEFLTGITDVSTQCFARAEDQRRFLGRLNMEEK
                     VVGFETRWRRRDGSEVEVSISARRVLDDGGRLRCYEGSLTDIGERKAKERAELARERA
                     EAASRAKSDFLAAMSHEIRTPMNGILGMAQLLARSGLAPGQAEWVDAIRASGSTLLGI
                     LDDVLDFSKIEAGQLTLESAPFSPAEALSALRPMLATMSAQKGLVLRLEIDPALPGGV
                     LGDRRALHQIVLNLAGNAVKFTARGFVAVRARCLPARAGRCRLGLEVEDSGIGIAEEA
                     QRRIFTEFTQADGSITRRFGGTGLGLAICRRLVDLQGGSLGVRSRPGEGSTFSCELEY
                     PLATPAPRASPRPHAAIGEARLHVLVVEDVELNRCIAGTLLEDEGHTVRFASDGYGAL
                     ELHEREDFDTILLDIHLPDLDGMEVARRIRRHPDARKARARIVALTASITTDEVARYR
                     AAGVDAVVGKPFDFDELIRSLREPPVPPAAAPGSALMDTRLLATHFARLGDERVRPMM
                     DMLATQGRAGLAEFAASADPATQRERLHRLVGMAANLGLAAFATRCRELEATPLPADL
                     RAQAGALRTLFEDSIAALHAFDPRDAQAALEAAAR"
     gene            61966..63387
                     /locus_tag="PKN25_00250"
     CDS             61966..63387
                     /locus_tag="PKN25_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNV89354.1"
                     /translation="MNKTPSLVIGGRKVAGDRGTLPVIDPALGEAFAECPNASPAQLD
                     EAVAAAARAFEQWQHSSCAERRALLEAIAARIEQNAPELAEIIVREQGKPLALAHMEV
                     GGAVAWTRATAALELPVEVIEDRPGKRIELHRRPLGVVGSITPWNWPLMIAVWHIMPA
                     LRAGNAVVIKPSELTPLNTLRLVELIDEVAPPGLVNAVAGGAALGRGISGHPGIHKIV
                     FTGSTRTGQDIMRNAADTLKRLTLELGGNDAGIVLPGTDIGAIAEGVFGSAFLNMGQT
                     CAALKRLYVHESQYEDMCRHLVAIAARQKLGSGLDEGTSFGPIQNRDQFERVCELVED
                     ARAAGARILCGGEPLPGKGYFYPPTIVADIADGTRLVDEEQFGPVLPVIRYRDVDEAL
                     RLANASTNGLGGSVWSGDLEAARALANRLECGTVWINGHAEVLPHCPFGGCKMSGFGV
                     EFGLEGLLEYTRPQLFNINLPAA"
     gene            63388..64362
                     /locus_tag="PKN25_00255"
     CDS             63388..64362
                     /locus_tag="PKN25_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89355.1"
                     /translation="MSAPLGFLRRPSAPASASVAASTASPPSLAALPATGVAIAVAMV
                     RPRALERLGADTPARAAFWSATLHDEALAAWLAPLRPTRLHVGSEFCERLLPEPAQLA
                     RTLAEASVAGLEVCLLTPVASPEVLRALDALFALLPPGAEVVVNDWGVALRLRERHPL
                     LQAVAGRILCRMLKDPRLGNPQYAPPAAHGFASPWTRRLLARLGIARVEIDLPLFASA
                     DSFAGVPLPLGVHLPFAFVAKGRMCRTAGLAREGPERFAARPFCHHECLGLAARTERT
                     RDGTHDAWETLHVGNTVFCRHSAANLATLASAVAAGRVARLIVPGETL"
     gene            64373..65428
                     /locus_tag="PKN25_00260"
     CDS             64373..65428
                     /locus_tag="PKN25_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNV89356.1"
                     /translation="MKIVAPIRQLDEIAALARAGADELYCGVTPREWAERFGGASANR
                     RPGGNLPSLAALAEAVALAHRNGVQLSLVLNAQQYSVEQIEFALAIAHRYVDMGGDAV
                     IASDPGLLLALAEAEPELRIHVSSVATCRNADGARLYRELGARRLILPRDITLDEAAE
                     IAAAVPDLEIEAFVLNDGCVYEEGSCNTLHLPGALGGPICLDRYAYAHRHRDGRPLSA
                     ALAARLQENDEAYRRWLWYRFSCGFTTTADGLPFGPCGLCAIPAFGRGGIHALKIAGR
                     EGPPERKLASVRMVRRILDAHDNGEAPAAVMARARGLRPAHEHCATGFMCYYPEVVSR
                     ASEAAQPLRDGRAAGAQ"
     gene            complement(65422..66030)
                     /locus_tag="PKN25_00265"
     CDS             complement(65422..66030)
                     /locus_tag="PKN25_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNV89357.1"
                     /translation="MPNVTAVMSTPNPLANKFMLDAPLYTGAPRHFSAGVAVVGDTLG
                     ESLLAVPGVVDIYCTGGFITITRDPGTPWSAIEPAVTELIEGHKARRVIGIANPAGPG
                     GAAGAAIGAGEAVAEEPVEVEADDAELLMRINQILDEHIRPFLDQDGGGLDVVKLKEF
                     TLTVRYKGACGGCPSSSTGTLFAINNLLQNYVDDRLLVEPIY"
     gene            66217..66798
                     /gene="rsxA"
                     /locus_tag="PKN25_00270"
     CDS             66217..66798
                     /gene="rsxA"
                     /locus_tag="PKN25_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNV89358.1"
                     /translation="MKEYLLLLLGTALVNNVILVRFLGLCPFLGVSGKLETALGMGLA
                     TTFVLTLTSGAAWVVEHALLQPLDMLHLRILTYILVIAAVVQFTEMAIQKASPVLHHT
                     LGIYLPLITTNCAVLGTALLNTQQGNGLVASMLYGAGSALGFTLVILLFAGLRERIAL
                     ANVPAAFAGAPIALITAGLLSLAFMGFAGLPVR"
     gene            66831..67370
                     /locus_tag="PKN25_00275"
     CDS             66831..67370
                     /locus_tag="PKN25_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNV89359.1"
                     /translation="MTMILSVVAVAALGLALGWLLGLASQWLGATTDPVVERIAEALP
                     GSQCGQCGFAGCAQAAAAVAAGDAPVTLCPPGGRAVAEKLAQILGATFDPGNLPDRGP
                     LLARVRTDACIGCSRCIKSCPTDAILGATKQLHVVLEEACIGCGACAEVCPTGGIDLE
                     GIPVTLRNWRWHKPGVGHA"
     gene            67406..68851
                     /gene="rsxC"
                     /locus_tag="PKN25_00280"
     CDS             67406..68851
                     /gene="rsxC"
                     /locus_tag="PKN25_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNV89360.1"
                     /translation="MKIFRFRGGIHPEGNKDPTACHPIARLPLPQRLYIPLQQHIGEP
                     AAPEVEVGDHVLKGQRLARHQGSVSAPVHAPTSGRVIAIGDHMAPHPSGLPVQTITLE
                     PDGEERWIETEPAVDPFALTRAEVSARVRDAGIVGLGGAAFPAAVKLDAGNRHPIDTL
                     ILNGGECEPYLTCDDRLMREGAGYVVEGVRLIANGLGAKRTVIAIEDNKPEALEAMRA
                     AAAAHPGIHVLRVPSRYPMGSEKQLVTTVTGREIPAGKLSAEIGVSVHNVGTALAVCR
                     AVRFGKPLVSRVVTVGGGAIRNPRNVVVPIGTPAGDLIDFCGGFTEPPTRVLMGGPMM
                     GQVLPGLEAPVVKGTSGIIALTAAEAHAASERACLHCGRCVDVCPVGLVPLELAARVR
                     AGRIDDAAELGMGDCLSCGCCAYVCPAHLPLAHLFNYAKGEIAARREAQRKAEETRKL
                     AEARRLRLAAEAAAKAARRPARPAETVETES"
     gene            68848..69921
                     /locus_tag="PKN25_00285"
     CDS             68848..69921
                     /locus_tag="PKN25_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNV89361.1"
                     /translation="MTSWTPNRSPVAAPHVHGRSNIPQVMRTVLLALLPATATGVFLF
                     GWPALATVLLAIAGCVAAEAAALAIGKRPLRHTLADGSAVVTGLILALCLPPWAPWWL
                     PLLGGAFAIVVGKQVFGGLGQNVLNPAMLARVALLVSFPLEMTAWVAPQPLFSPTAPG
                     LLDGLAITFGGGAVPDAMSAATALAPLKHGGDFAAGEALLALALGTVPGSLGETSALA
                     LLAGGALLVARRIVPWQIPAALLGTLALLAALFNQIDPAHYPPPLLHLLGGSAVMCAF
                     FIATDPVGAPVTPLGRAIFGAGIGALIYVIRTWGGYPEGVAFAVLLMNAATPLIDRLV
                     RPRRYGRLRTGNPIPLAPKEDGR"
     gene            69918..70574
                     /locus_tag="PKN25_00290"
     CDS             69918..70574
                     /locus_tag="PKN25_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNV89362.1"
                     /translation="MSSIAAGGLRQRPGLLLGAFALGCSAMLGMGDLATHEEIRLRQA
                     EDLRNALDQTLSAVPHDNNPAADTVAVEDEGRAVTVYRARLAGEVRGVAFNWVSEAGY
                     GGPITLTVGIGRDGRIASVRVLSHSETPGLGDKIDVRKSDWIRGFDGRGLDTGTRWAV
                     RKDGGEFDQFAGATITPRAVVGAVHASLKFFAGNRARLLAAPAGPGGDAAATRSETPS
                     "
     gene            70571..71251
                     /locus_tag="PKN25_00295"
     CDS             70571..71251
                     /locus_tag="PKN25_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNV89363.1"
                     /translation="MSEVRRVVRDGLWDNNVVFAQMLGMCPTLATTGSATNGLGMGLA
                     TTVVLVSSGVLVSLFRNVIAREVRIAIYVLLMSTVVTLVDMAMNAWLHELHKVLGLFI
                     PLIVVNCAIMGRAEAFASKRAPGMAALDGLMMGIGFTLALMVLGGVREAIGQGTLFAD
                     ASMLLGQHFAFLELRLEGYRGVLLMILPPGGFLALGFLVAARQRMLARARARSLGASA
                     GAGITAGT"
     gene            71290..71604
                     /locus_tag="PKN25_00300"
     CDS             71290..71604
                     /locus_tag="PKN25_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNV89364.1"
                     /translation="MKISVSYAHPEKPAWMRLDVDEGCTLRAAIERSGILARYEDIDL
                     ERNKVGIFGKIKPLETVLAEGDRVEIYQPIVIDPKTAPRRKRTGADGGDGTEGAERPE
                     GN"
     gene            complement(71634..72092)
                     /locus_tag="PKN25_00305"
     CDS             complement(71634..72092)
                     /locus_tag="PKN25_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNV89365.1"
                     /translation="MNQEIAGRAGRVRESANATTYTISDLAKEFDITTRAIRYYEDQG
                     LISPARRGSRRIYSRRDRVRLSLILRGKRLGFSLQETRELFDLYDNAPGEQAQMSHFM
                     ELLGARRAMLERQMEDIRAILDEIGAAEKEALRSIAARAPHPKAATSPSA"
     gene            complement(72305..72946)
                     /locus_tag="PKN25_00310"
     CDS             complement(72305..72946)
                     /locus_tag="PKN25_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV89366.1"
                     /translation="MARGKAPTFQLQRATILDAAAGLFAAEGFHNASMAEIARACGVS
                     KALLYHYYRDKEHILYDIAAGHVEDLLAIVADVEAAAESPRERLERLILRFMQAYEGG
                     RRQHVVLIQDVKFLSPEQRARIHEQERRVVHAFALAIEAVEPGLRVVALDKAVTMALF
                     GMMNWTFTWLRPDGPLSYEDMARVVARIFLQGVAGFAAQAGHPSAAAVEIALP"
     gene            73402..74010
                     /gene="paaY"
                     /locus_tag="PKN25_00315"
     CDS             73402..74010
                     /gene="paaY"
                     /locus_tag="PKN25_00315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HNV89367.1"
                     /translation="MPCYEIDGVRPVVHPTAYVHPDAVLIGDVFVGAGCYVAPLASLR
                     GDFGRIVLEEGSNVQDACVLHSFPGIDTVVEVNGHVGHAAVLHACRIGRNALVGMNAV
                     VMDHAEVGEGSIIAAAAFVKAEMKIPPGVLVAGVPAKVLRELTEQERKWKVIGTDCYQ
                     NLARRSLASLKPCEPLREGDRERARFEMPDVMPLSDARRRAG"
     gene            complement(74038..74976)
                     /locus_tag="PKN25_00320"
     CDS             complement(74038..74976)
                     /locus_tag="PKN25_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV89368.1"
                     /translation="MSLRDFDLNLLRVLLAVYEAGSVSRAAEHLGLSQPTVSSALGRL
                     RRALGEPLFIKTASGVTPTARTHALASSARAMLARLETDFFVADRFDPAKYEGNLTVA
                     LSDVGELVFLPRILDSVRRLAPLAMLDSVTLAPGELMRAMEDGEVDLAIGYYPDLDAA
                     GFYREKLLDHRFVCLLRADHPVQDRQLSLERFLSLEHAAVRAKGRSQEIFEKYLERLR
                     IQRRIVLRTPHFMSLPGIIGRSDLVVTLPHAIGHYFTRVGEKVRMAEPPLAIPHIELY
                     QHWHRGFHHDPRNRWLRTLVAGLFSDGQDEWPYPFA"
     gene            75187..76620
                     /locus_tag="PKN25_00325"
     CDS             75187..76620
                     /locus_tag="PKN25_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV89369.1"
                     /translation="MNAAEWLLAGDPARIALIEGDVRIDYAGLRADSIRSAAALLDAG
                     LRPGDACVLALPDGIEWAAAFIGMLWAGVRPIAINPRTATTQLADLMLDSGAAAALLE
                     DEAARALGDKRAIDLGEWRRRVERATATPAAAQAADDDPAFLLYSSGTTGRPKGILHA
                     HRAIRDAHVFARDLLGARPEHRFYSSSKLFFAYPLANAFFAGLRLGATVVLDPEWPDP
                     ARVAAMVERHEPHIFFSVPTLYRRLIDAGVRFRGVHAAVSAGEACPPALARDWQAMTG
                     VPLVNGYGTTETLSLVLYRTPEMDAACPTPLTEIHPEQLTSGELETWRLWFSHPAVAL
                     GYTRVVTHDSARFADGRFAPGDVFRRAPDGEGWLFAGRSDQLVKVFGRWVDVVAVEQA
                     VQERMRGKAEEVCVIPAQGEDADMIRLHLFAIPGDLPPPQVLAAAQAAIESLPPYQRP
                     EKIHLVDHFPRTDTGKLRRNELARSTG"
     gene            complement(76654..76977)
                     /locus_tag="PKN25_00330"
     CDS             complement(76654..76977)
                     /locus_tag="PKN25_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNV89370.1"
                     /translation="MKVILDLCVVPLGVGLSVSSYVAACERVIREAGLVSQLHAYGTN
                     IEGEWDEVMAAVKRCHEVVHAMGAPRITTSIRLGTRTDREQTMQDKIDSVERALDRAA
                     SGPGA"
     gene            complement(77014..77610)
                     /locus_tag="PKN25_00335"
     CDS             complement(77014..77610)
                     /locus_tag="PKN25_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNV89371.1"
                     /translation="MLLNKPFGVLCQFTDEAGRRTLKDYVEVPGVYAAGRLDADSEGL
                     VLLTGDGALQHRIADPRHKLAKTYLVQVEGEIDEAALAILRAGVDLGDFRSLPCAARR
                     MPAPDWLWPRDPPVRFRKSVPTSWIEIVLREGKNRQVRRMTAKVGHPTLRLIRVAIGP
                     WTLDGLGVGEWRELAGAEIAEVLPAGAPAAARRGRLRR"
     gene            77783..78271
                     /locus_tag="PKN25_00340"
                     /pseudo
     CDS             77783..78271
                     /locus_tag="PKN25_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886403.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        12042 a        28156 c        26272 g        11760 t
ORIGIN      
        1 cggcgagctt ggcggtggtg gtggtcttgc cgacgccggt ggggccgacc agggcataca
       61 cgccgccacg gtcgagcagg tcctcgtccg aggcgcaggc ccgcatcagg cgatcgaggg
      121 cggcacccac ggcctcgcgc gcctcgatgc ggtcggcgtc ctcgtgcacg gcctcgacga
      181 gcttgcgcgc gatcgccgcg gagaacccgg cctcgagcag ctctccgagc atggccgcgc
      241 gcgccggcgc ctggcggcgc gtctcgcccc aggcgaagcc ggcgagctga cgttcgatca
      301 tgccgcggat gccggtgagc tcggccatca ggcgggcgtt ggtctcctgc agttcgcgca
      361 cgcgcgcgct gccgaattcg tcggcgtcgg aacgcgcggc acgaccggag aacaccgccg
      421 gaccgctcgg cgccgggcgg gcggcggtct gggccgcggc cgccgcgcgc agcacgccgt
      481 cgcggctgcg cagcgcgtag tcggcggtct cgatgcgtgg cggattgaag gcgcgcacca
      541 ccgtcgccgg ctcggcggcc ggctgcgcga cggggcggga agcgttgcgc gcggcgctgg
      601 ccgccagcgc ggaaaggctc acgcggaagt cggcgtcgtc ggcgctgcgc gccgccgcgg
      661 ccggcatctc gaccgctgcc ggccggacct ccccgcccgc acgcgccacc accgccgccc
      721 gattggccgc ctgcagcgcg ccgaccgcct cgcccggcag cgcgaggatc tccaccccgc
      781 cttcgacggc gcggttggac agcacgatcg catccggacc gagggcttcc ttgagcgcgc
      841 ggagcgcctc gcgggcggtg ggggcaaagt agcgtttgac gttcatggcg aatctcccgg
      901 tggccgtcgt cgaccactgc ttgatgagga ttatcgcgag gccgggtcaa gcgcctgggg
      961 tggaataaag cgggtttccg gggtcaattc gggagacggg gagcgcgcac cggcggcagc
     1021 gatcgtggcc ccgcagccgt gccgggaagc gcgagcattg tccgcaggcg cgccgcgagt
     1081 cgcgatcgcg gcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacacgg
     1201 acgaaccgcc tgatcgcgac ttgcgtcgct cccacggcga ctccgcaggc gcgtgggtgc
     1261 tcagccctgc gcgcccacca tcgcggtgac gcggatggtg cggttctccg gcacctcgct
     1321 gttggagacg accttcagcg acggcacggc gcggcgcagg aagcgcgaca gcagcaggcg
     1381 cagttgcgcg ggcaccagca acaccggcgg caagccgaca tcctcctggc gctgggcgat
     1441 ctgcgcggtc tggcgcagca aggtatcggc caggcccggt tcgatcgccc cgccctcgcc
     1501 gcccgcagcc atggcctgca tcaggatgcg ctccagcccg ggctcgagcg ccatcacctg
     1561 gacctcggca ttgcccggga acaggccctg caggatggcg cggccgagcg cctcgcgcac
     1621 ccgggtggtg agctgggccg gatcctgcac gcgcggcgcg ttctcggcga gcacctcgat
     1681 gatgctgcgc atgtcgcgga tgttgacgcc ttcctcgagc aggttctgca gcacgcgctg
     1741 cacggtgccg agcggcagca gcttgggcac gaggtcctcg accagcttgg gcgattcctt
     1801 gccgaggtgg tcgagcaggg cctgggtctc ctggcggccg agcagctcgg cggcgtggtt
     1861 gaggatgacg tggttgaggt gggtcgccac cacggtgctc gcgtccacca cggtgtagcc
     1921 gagcgcgtgc gcctgctcgc gcatcgaggc gtcgatccac accgcgggca gcccgaaggc
     1981 ggggtcggtg gtctcgcgtc cggcaagcgg gccggacacg cgccccgggt tgatggcgag
     2041 gaactggcca ggataggcat cgccactgcc gacctcgacg cccttgaggg cgagccggta
     2101 accgttgggc ttgagctcga ggttgtcgcg gatgtgcacg ggcgcggaca ggaaaccgac
     2161 ctcctgggcg aacttcttgc gcaggccgcg gatgcgcttg agcagctcgc cgtcctggcc
     2221 cttgtcgacc atcgggatca gccggtagcc gacctccagc ccgagcacgt ccacctgcga
     2281 gacgtcgttc cagctcgcct ccagattgtc ggcggcgggc atcggcacct gcgccgcggc
     2341 ctcgggcgcg gcggcggccg cggcttcctc ggcgatgcgc ttgcgccgca tccaggccat
     2401 gccgccgagc gtggccgcga gcagcaggaa gaccaggttg ggcatgcccg gaatcaggcc
     2461 gagcacgccg atgatcgccg cggtcagcac catgacctgc ggattggaga acagctggcc
     2521 gatcaccagc ccgccgacat cgccctcgtc gcccacccgc gacaccacca gaccggccgc
     2581 gaccgagacg atcagcgagg ggatctgcgc caccaggcca tcgccgatgg tgagcagggt
     2641 ataggtgtgg gcggcgtcgc ccaccgccat gtcgtgctgc atgatgccga cgaagaggcc
     2701 gccgatgatg ttgatcgcca ggatgatgat gccggcgacc gcgtcgccgc gcacgaactt
     2761 ggacgcaccg tccatggcgc cgtagaagtc cgattcctgc gcgatctcct tgcgcctgcg
     2821 cttggcctcc ttctcgtcga ccaggccggc gttgaggtcg gcgtcgatcg ccatctgctt
     2881 gcccggcatc gcgtccaggg tgaagcgcgc cgacacctcg gcgatgcggc ccgcgccctt
     2941 ggtgatcacc acgaagttga tcacggtgag gatgatgaac accaccagcc cgaccgcggt
     3001 gttgccgcca acgaggaagt tgccgaaggc ctcgatcacc ttgccggcgg catccggacc
     3061 ggcgtgaccc tctagcagca cgatgcgggt ggaggcgacg ttgagcgaca ggcgcagcag
     3121 cgtggtgacc agcagcacgg tgggaaaaac ggagaagtcc agcggccgcg tcgagtacat
     3181 cgccaccagc atcaccatca ccgagatcgc gatgttgaag gtgaagaaga cgtcgaggag
     3241 gaagaccggc agcggcagca ccatcatcga caggatgacg aggatcagca gcggcgcggc
     3301 gagcgggcgc aggttggcag gcgcgagcag ctggcgcagc ttgagggtgt cgttcagggc
     3361 catggttcag gcttcattcc gcgggcacgc cgggatccat cccgacgggc accggcaagg
     3421 cagtgggggc ggcaggcggc aggccgccct gcttcatcca ggcgtcgagc tgatagacat
     3481 aggccatcac ctcggcgacc gcggtgtaca gcgcggccgg gatcgcctgc tcgagctcgc
     3541 aatgcttgta tagcgcgcgc gcaagcggcg gcgcctcgag cagcgggact tcgctctcct
     3601 tcgcgatctc gcggatcttg agggcgagct ctccccgccc cttggcgacc accaccggcg
     3661 cgcccatctt cgcggcgtcg tacttgaggg cgacggagaa atgggtcggg ttggtgacca
     3721 ccacgtcggc cttgggcacc tcggccatca tgcgacggcg cgccatctcg cgctgcatcg
     3781 cgcggatgcg gcccttgatc atcgggtcgc cttcctgctc cttgctctcg cgcttgacct
     3841 cttccttcgt catgcgcagc ttcttgtggt actgccagag ctggaagggc acgtcgatcg
     3901 cggcgagcag gccgaggctg agcacgatca gcagggtggc gaagacgatg gtggcggaaa
     3961 aatccgccat gccggtctcg aggggctcga ccatcaggct gaacaggtgc tcgaactcgt
     4021 accacagcac cagggcggcc acgccgccga ccagtacgga cttcaggatg gccttgaaca
     4081 tctcgaccag cccgtgcagc gagaacatgc gccccagccc ctgcagcggg tcgaggcggt
     4141 caaacttgag cccgagcacc ttgggtgcga agacgagacc gccgagcacc atcggcgcag
     4201 ccactgcggc gaccatcagc gcgacgaaga gcggcatcag ggtgagcagc gcgccgccga
     4261 gcatgcgctc gagcatgtcg agcatgacgt tctcgtcgaa ggcggcgcgg cggtcgaagg
     4321 cgaaaccgtc gcggaacagc cccatcaccc gccccgccat ccagcccccg agcaacagca
     4381 aggtcgccgc accggtcatg gtgacgagga aggtcgacag ctcccgcgat tgcgggacct
     4441 ggccttcctc acgcgcctgt tccagcctgc gtgacgaggg gtcttctgtc ttctcgaggt
     4501 cgctgtcttc ggccacggcc ggctccgtcc gccgtcgcgc cgcaccgcag gaatacgggc
     4561 gcgcggcgac gtgtgcggat tatcggccga tgccggcaag ggccggacgc agaaaacgtg
     4621 gtggaaagcg gcgcaattcg gcgctttgtg cgagcgccgc cgagaccgcg ccgatcatcc
     4681 cggccgtgtc ccggcgcggg ctttcgatcc gcccgggcgc ccttcagcgc ggcgcgggca
     4741 ggcggtcgat accgcgcagc agcgcacccg cagcgatccc gcgctcttcg aaccagccgg
     4801 cgttcatctc gagcgcgaag cgcgccggcg cggccgcgca gtggttgacc tcggtgcgcg
     4861 gcgccatgcg ctcgatgttg aggatcttcc ccctggcatc gatgaaggcg accgagagcg
     4921 gcagatgcgt gttgcgcatc cacatgcagt gcgccccctc ctccggaaac acgaacacca
     4981 tgccgcgatg cagcggcatc gccacgcggt gcatcagccc ggtctggcgt gcctctgcgg
     5041 tatgggcgac ctcggcctcg atgcggtaca tgccggcccc gagttcggcc accggcaggc
     5101 tggcgccccc cccctgcgcc gcggcggaga aggcggccgc gcacaggacc gaggcgatcg
     5161 aggaacggag aacggaggag cgtttcttca tcgcggaaca ggcccgaaaa agcatggagg
     5221 ccgcgagttt agcgcgcgcc gaggagtaag gcgcgagacg tgaggcgcaa ggagcggaac
     5281 cgccacagcc gggaagtccg cgagcgcccc cggacccacc gcgcctcgca cctcacgcct
     5341 cactccttac gtcgccagca tctcctgatg cttgccgccc acgccgaggt aggcatcgat
     5401 cacgcgccgg tcgccggcga gctcggaggc ggggccctcc atggcgatcc cgccggtctc
     5461 gagcacgtag gcgtagtcgg ccacctgcag cgcagcccgg gcgttctgct cgaccagcag
     5521 gatggacacc ccgcggctgc gcagttgcgt gacgatgcgg aagatctcgc gcgtgatcag
     5581 cggcgccagc cccaggctgg gctcgtcgag catcagcagc ttgggcttgg ccatcagcgc
     5641 gcgaccgacc gccagcatct ggcgttcgcc gcccgagagg gtgccggcca tctgcgtgcg
     5701 acgttcgcgc aggcgcggga agagcgcgaa gatctcttcc atggtctgcg cgtggtcgcg
     5761 caagcccatg cggtagcgct ggaaggcacc cagcacgaga ttgtcctcga cgctcatctc
     5821 gccgaaaagc tcgcgcttct ccgggaccag gttcatcccg ctcgcgacca tggtctcgac
     5881 ctccggcgcc accatcgcca cgctgtcgaa ttcgatgcga ccacgcgagg gcagcagccc
     5941 catgatcgcc gacagcatgg tggtcttgcc ggcaccgttg gggccgatca cggtgacgat
     6001 ctggccctcc ccgaccgtga tgctggcgtc ggtgagcgcc tcgaccttgc cgtaggaaac
     6061 gcacaggcct tcgacccgca ataccgggcg atcgctaagg ttcttcgtcg ccatcagtct
     6121 tctcctccga gataggccgc gagcacggcc gggtcgcgct gcacgtcctc cggcaggccc
     6181 tcggcgatct tctcgccgaa ctccatcacc accacgcgat cgaccaggcc catcacgaag
     6241 tccatgtcgt gctcgaccag cagcacgccc atgccttcgc cgcgcagctt gcgcagcagg
     6301 tcgccgagcg cttccttctc cttgtagcgc aaaccggcgg caggctcgtc gagcagcagc
     6361 aggcaggggt cggaggcgag cgcacgcgcg atctcgagga tgcgctgctg gccgagcgcg
     6421 aggctgccgg cctcgtcgaa catgtgctcg cccaggccca cgcgctcgat ctggcgcgca
     6481 gcctcggcga gcaggcgcgc ctcctcggcg cggtccatcc gccacgccgc cgacagcacg
     6541 ccgctcgtgc cgcgcatgtg ggcgccgatg gcgacgttct cgagtacgct catggtgggc
     6601 agcaggcgca cgtgctggaa ggtgcggctc atgcccatgc gcgcgatcgt gcgtgagccc
     6661 ttgccgacca ccgactcgcc gaggaaacgg acttcgcccg aggtcggcgt atccacgccc
     6721 gaaacctggt tgaacatcgt gctcttgccc gcgccgttgg gaccgatcag ggcgaggatc
     6781 tcgcccgccc gcacctccag gctcatgttg ttgttggcga gcaggccgcc gaagcgccgc
     6841 gtgaccgcct tggcctcgag cagcaggctg ccggcggccg gcagctcgcg ccgcggtagc
     6901 gccaacgcat cgcgatcgac cgcccgacgc tgtgccgcga ccgggatgaa gcgcttgaac
     6961 cccgacacca ggatcggcca cagcccgccg cgtgccttgt gcagcaccag caccatcagc
     7021 acaccgaaga agatcacctc gaagttgccg ctggtgccga agagcttggg cagcaggtcc
     7081 tgcagccact gcttgagcac ggtgatcacg cccgcgccga cgagcgcgcc ccagacgtgg
     7141 gccgcaccgc cgacgaccgc catgaacaga tactcgatgc cgatgtgcag gccgaatggc
     7201 gtcggattca cgaagcgctg catgtgcgcg tacagccagc ccgaggcgca agcgagcaag
     7261 gcggcgagga tgaagatcac catgcgcgag cgcgcggtgt cgacgcccat cgcctcggcc
     7321 atcaccatgc cgcccttgag tgcgcgaatc gccctgccct cgcgcgagtc gagcaggttg
     7381 ctggtggtca gcacgccggc gagcacgaag agccagacca ggtaatacac ctcctcgccc
     7441 tggcgcagct cgtagccgaa cagcgagatc ggcgggatgc cggtgaggcc ggtgtggccg
     7501 cccagaaagc ccatggtgcc gaacaggaag tacaggctca ggccccaggc gatggtcgcc
     7561 agcggcaggt aatgcccgga caggcgcagg gtgagcttgc cgatcaggaa ggccaccacg
     7621 acggtgagca ccagcccgac gagcagggcg agccagggcg agccgcccgc ccaggccagc
     7681 cagccgggca gttccgccga ggtggtgagc agcgcggtgg tgtaggcgcc gaggccgacg
     7741 aaggcggcct ggccgaagct ggtcaggccg cccacaccgg tgagcagcac cagcccgagc
     7801 gcgaccatgg catacaggcc gatgtagttg agcagcgtga cgtagaaggc cgacagcacc
     7861 agcggcgcca ccgccagcag agcgacgaac gcggccatca ccaggcgcgg agagagcgcg
     7921 ggtttcattc ctcttcctcc acgtggtggc tggtcaggga gcgccagacc aaaaccggga
     7981 tgatcagcgt gaacaccagg acctccttgt aggcgctcgc ccagaacgaa ctgaaggatt
     8041 cgagcaggcc gaccagcacc gcgccgaagg ctgcgagcgg atagctcgcc agcccgccga
     8101 tgatcgcggc gacgaaaccc ttgaggccga tcaggaagcc gctgtcgtag tagatcgtcg
     8161 tgatcggcga gatcagcacg cccgacagca cgccgatcag cgcggcgagc aggaaggtga
     8221 gcttgccggc gagcgcgggc gggatcccca tcaggcgggc gccgttgcgg ttgatcgcgg
     8281 tggcgcgcaa ggccttgccg tagatcgtgc ggccgaagaa cagcgcgagc gcgacgatca
     8341 gcacgaccga agccgcaatc acccagatcg tctgcccgct caccgtcagc gggccgacct
     8401 cgaagctcgc atccgagagc ggtggcgtgc ggtaaccctc ggcgccgaag aacagcaggc
     8461 ccaggcccac catcgcgacg tgggacgcga ccgagacgat cagcagcacc aggaccgtgg
     8521 cctcggcgat cggctgaaag acgatgcggt acatcatcgg tccgagcggc accaccacca
     8581 gcatggtgag caggatctgc accgcgaggc cgagttcggt tagcggcaac aggcgcagca
     8641 gggcaaggac cgcaagcggg tagccgatat tgaccgcgag gacgctcggc agcttgcgcg
     8701 cctgcccggc acgcagcgcc ccgagtccgt cgagggcggc gacggcgacc ccggcaccga
     8761 gcagcagcca ggccgtggcg gggaagacac cgttaccgat catcgcaagc gtgagcgcgc
     8821 cgtaggcgac gaactctccc tgcgggatga agatcacccg ggtgacagcg aacaccagta
     8881 ccagcgcgag cgctagcagc gcatagatgg cgccgttggt gatgccgtcc tgaccgagga
     8941 tcagtgcaat ctgaaaatcc atggacgcga gcctccgcgc gccccggccg ggagccgaag
     9001 cgcgatcgag tggaccgact gatgggaacg gggcggtcgc cgggccgcaa gcccggcgac
     9061 cgcgcgggag caccgcttac tggacgagct tccaggcgcc cttctggatc tgcaccatca
     9121 cgcgcgcgcg ctggtcgagg ccgaggtggt cgttcggacc catcgtgaac acgccgtggg
     9181 ccccggggac ctccttcatg ttctcgagcg cgtcgcgcag cgccgcacga aactcggcgg
     9241 tgccgggctg ggccttcttg agcgccgcgg gaacggccgc ctgcagcatc acgccggaat
     9301 cccaggcgtg ggcgccgaag gtcgacacgc tgcccttgcc gtgcgccgcc tcgtaggcct
     9361 tgatgtaggt catcgcgctc gccttgaccg ggttgttgtc gggcaactgc tcggcgacca
     9421 gcaccggacc ggcgggcagc agcgtgccct cgcagtcggc gccgcagacg cgcaggaagt
     9481 cgttgttcgc cacaccgtgg gtctggtaga ccttgcccga ccagccgcgc tccttgagcg
     9541 tcttctgcgg cagcgccgcc ggcgtgcccg agcccgcgat gaacatggcg tcgggcttgg
     9601 ccgccatcat cttgagcacc tgcccggtaa ccgaggtgtc ggtgcgggtg aagcgctcgt
     9661 tggcgacgat cttgatcccg cgcgcctcgg cgaccgactt gaactgctcg taccagccct
     9721 cgccataggc gtcggcgaaa ccgatgaagg ccacgttctt gacgttgttg ctcgtcatgt
     9781 gggagacgat cgcggtcgac atgtgggagt cgttctgcgg cgtcttgaac acccagcgcc
     9841 gcttctcgtc caccggctcg acgatgcggc tggaggccgc ccacgcgatc atcggggtct
     9901 gcgattcggc ggcgacgtcg atcatgccga gcgagttggg cgaggtggtc gaaccgagca
     9961 ccacgtcgac cttgtcctcg gagaccagct tgcggatgtt cttgaccgcg ctggtggtgt
    10021 ccgaggcgtc gtcgaggacg acgtaggtga ctttctgccc ggcgatctcc ttgggcatca
    10081 gctcgacggt gttgcgctcg gggatgccga gcgaggcggc cggcccggtg gccgagacgg
    10141 tgatgcccac cttgagctgg gcatgagcgg cgcccgccgc aaaggcaaga ccgagcgcga
    10201 cgaacagcgt cttcttcagt ttcatggtat ctcctccggt cgttatggat taggcgacca
    10261 gcctgaccat gacgggccgc ccttcttgcc acccgatgaa acccgatacc tgcagttcca
    10321 cgaaaagcgc ctcaatggta gacgattccg ggccctgcgg gcgtgatgca tgctacgcgc
    10381 aaagcggctt cccaggccgc tttgcgatgt ttcagacgta ctgcccggcg tccacccgga
    10441 tgacctcgcc ggtgatcatg cgcgccatgt ccgagagcag gaagacgacg gctgcggcga
    10501 tatcgtccgg ggtggcgagc tcgcgcatca ccgactcctg cagcgcgcgc tcgagcacct
    10561 cggccggcag cgcgcgcgcc atctcggtca tcaccatgcc gggcgcaacc gcgttgacgg
    10621 tgatgccctt ggggccgagt tcgcgcgccg cggtcttggt gaggccgatc atgcccgcct
    10681 tcgccgcggt gtagttggcc tggccgaact tgccgcgcag gccgttgatc gaggtgatgt
    10741 tgacgacccg gccgcggccg gccgcgatca tgcccggcgc gcaggcgcgc agcatgtgga
    10801 atgcgccgct caggttgacg tcgagcaccg agcgccattc ctcgtcgctc atcttgagca
    10861 ggctgcggtc gcgcgtgatg ccggcgttgt tgaccagcaa cgtcgccggc tcgggcagct
    10921 gcgccaccgc gtcggccacc gactgcgcgt cggccacatt cacccgatgc agcacgcccg
    10981 ccgactcgtt gctgtcggtc atgtcgaaga catgcacgcg cgcgccggag gcgaccaacg
    11041 cgtcggtgat cgtgcgtccg atcccgcgcg cgccgccggt gacgatcgcc accttcccag
    11101 ccagttctcc gctaaaaccc atgagccgct tcccctctgg atgtgcatat gcccggagcc
    11161 cgcttgcgcg gaccccgggc cggttgatgc cgagcgctca gacgagcggg cgcgacagcg
    11221 agagctgctg gccgggacgg aagccgcagc ggtcgaggaa gcgcagcagg tcgaagcggt
    11281 tccactcgac ctcggtgcgc accgactcga cgtgcagcga ggtcaggttg gcgagcagct
    11341 gcgacaccag cgcacggccg acgtgcttgc cgctggccgc cgcatcgacg ccgatggtgt
    11401 cgatcacggc agcagtggcc gcgcggccga actcgccgaa gtcgacccgc gccatcagga
    11461 agccggcgac catgccgtcc tgctcggcga cgaccgagac gcggacgccg gactcgccga
    11521 gcgcctcgtc gaacttgcgc cggtagtagg cgctgcggtc gtggcctgtg gtgcggcggt
    11581 cgatgcgcac gacggcgtcg aagtcggccg cggtcagcga gcgcaccggg atgcggtcgc
    11641 gcgacaaggc ttcggcgtca tcctggctgg cgtcgctgta gtcgggctcg gtgttgaact
    11701 ggtccagcag ttgcttggtg gtgggatgcg gacgcgtggt catcgctgat ctccaggttg
    11761 tcgtttccgg acgtgaactc agaagttcac gtattcggtc gggcgctcga gcaccagccg
    11821 cggcgacagg cggaagccgg ccgcggagaa gaactcgacc agaccgggac gatcccattg
    11881 cgcctgggtg cgcatctcgc tggcgccgcg ttcgcgcgcg ctgcggtcga actcgccgac
    11941 cagcttgcgg gcgaggccct ggcgctgggc cgcggggtcg atcgccacgg cgtcgagcac
    12001 cgcgacgcgt tccaggccgc cgaattcgcc gtcgagcagg tggcccagca ggaaaccggc
    12061 gacctcgccg ccacgctcgg ccacgcagga gaagaatccc gtggggctct gctgctcggc
    12121 ctggaggcgg cgctcgaaga agccggcgcg gctctcgccg ccattggtcg cgtcgaggcg
    12181 ggtgactgcc gccaggtcgc cggcggccag cggccgcagc ttgatttcgg tattcgcagt
    12241 cattgcgtct ccctgagatg actggggcgg agggccgcca tcggccccct ccgccggtgg
    12301 atcagtgcag gctgtcgtac ttggcctgca gttcgtcctg cgattcgcgg tagtccgggt
    12361 gatccttgat gcagtcggcc gggcagacga gcttgcactg cggctcgtcg aaggcaccga
    12421 cgcactcgga gcacttgccc ggatcgatca cgtagatcgg gttgccggcg gtgatcgcct
    12481 cgttcgggca ctcctcgaca caggcatcgc acgcggtgca gtcgtcattg atgagcaggg
    12541 ccatgatggc ttcctcctgt aggctggttt caggccgcgg cggcgagctg ctgcccgccc
    12601 aggcgcgcga gtttctcgtg gcggaaggca ccccacaggg ccgcgccgat cgcaccggcg
    12661 aagggcgagt ccgggtggtt gaacgcagtc acgttcatct tctgctcctt gaccgactcg
    12721 ttgagggtgt tgagcaggcc ctcgtcgagc gccaggccgc cggtgcagag caccacgccc
    12781 tcgcgcgcgc cgatcgactt gagcagcttg gccaggcgac cggccatcga gatgtggatg
    12841 cccttcagga tgttgggcgc cgtgatgccg cgcgacacca tgttgatgac gtcggtctcg
    12901 gccagcaccg cgcagatgct cgacaccacc tcggggttgt cggcctggtt cgacagcgag
    12961 ccgatctcgt cctgggcgat gccgaggtag cgcgcgatgt tctcgaggaa ctgccccgag
    13021 cccgaggcgc actggctggt catcttgtag gtctcgacct tgccgcgctc gtcgttgcgg
    13081 atcgcccgcc cgtgcaacgc gccgatgtcg agcaccgcgc gcgcttccgg gttgaggtag
    13141 accgcgccac gcgcatgggt cgtcatggag tagaagtggc cggtgtggaa gtggaggctc
    13201 tcgccctcgc cggtggtggc gacgtaatcc acgtcggacg ccttgaggcc ggcctcctcg
    13261 aggagcgcgt ggtaggcctc ctcggcgagc ttgaagggat cgcgctgacg gatgcggtcg
    13321 ttgcgcttgc ccagccactc ggtcttgtcg ccctcgacgc ggaagaggac ggtcttgacg
    13381 gcgccggtgc cgatgtcgat gcctgcggtg atggtcattt tcaagcctcc acgacggcgc
    13441 gacgggcgaa ttcggaagcc ccaagcgcac cggtatagat cgaatcgggg tcgatgttga
    13501 tcttcacctc cccgtagttt tccttgacga gctggcgcaa ctccttgacc gcggcctcgt
    13561 tcttggccac gccgccggtg aaggtgaact cgtcggtgat gccgcccgag cgcgagatga
    13621 tcgacatcgc gcgcagcatg atggcgcggt gcaggccggc gaggatgtcc tcgcgcttgt
    13681 cgcccagcgc caggcggtcg cgcagctcgg cgccggcgaa cacggtgcag gtcgagttga
    13741 tgcggatcga cttggtcgcc ttcatcgcga gcgggccgag ttcgtgcagg cccatgttca
    13801 tctcgtcggc gacgtagccc aggtagcggc cggtgccggc ggcacagcgg tcgttcatct
    13861 ggaagttcac cacgatgccc ttctcgtcga tctggatgcc cttggtgtcc tggccgccga
    13921 tgtcgagcac cgtgcgggtc ttcggataca tcaggtgggc gcccaggccg tggcagagga
    13981 tctcggagcg gatgtgctcc ttcgggaagg gcaggcggac gcggccgtag ccggtgccga
    14041 ccacatagcg ctcgaccatg tccacatcga gcgccgcatc gatcggcgcg ccgagcgcgg
    14101 cggtgtcgag ggcgctggac atctccccac gaacccgctc catcgcggtg cggaacttgc
    14161 ggttcatgtc acccgaaggc ggacggttct cgacctcgat gatcgacttg tcgtacatgt
    14221 gcagcagggt gtcgaagggg atcccggcat cgcgcgccac ttcctcaccg atctgcatga
    14281 agcgcgaacc ggccagatcg cggaagaagt ccgatttgcg ctgcgccgtg ggtgcgaaca
    14341 gcttgtcggc ttcttcctcg cgcaggcggc ggaagacttc ggtcagcgca ccgacgatca
    14401 ctttttcctt gccggcgaag cgcgggcctt cgacgttggc gatgcaggtc tgctcgaggt
    14461 cgccgagctg ctcgacgaac tgctcgcggc ggaagttgcg cgtcaggccg gcgaggatct
    14521 cgtctacccg accggcgacg gccggcacct tctcgagctc gcgacggatc aggctcaggc
    14581 gcgcatcgat gaacgcctcg agcttggaga cgcgtgcggc ggtgtcatag ttcgagcgcg
    14641 agttggtgat gccgcgaccg agcacctgcc ccttgtcgtc catgacgacc gccttggtcg
    14701 tggtggaccc cagatcaatt ccgacgaaac attccatggt tcgctcctta cgcggctgcc
    14761 agcgacgcgc cgctgcgttt ctgatcgacc atctggaagt agctttccag acggttcttg
    14821 acgtttgcgt gggagaagta gcgcggatcg acgaggtcgg tctcgatgaa cgccgccggc
    14881 ttgccggtcc gcttctcgat ctcgcgcatc atcagcagct gacccgccga gaagctgttg
    14941 cagctcttga tcgagttgat cagcaggcca tcggcctggt agtcgttcat gtacttctcg
    15001 agcagttcga cgcgctgcgg caggttgttg ttggtgtagc aacccaggca gtagtcggcc
    15061 agcgtgccca gcggatcgtt cgggtcgtga cggaagccct ggtcgtagag gccgcccacc
    15121 ttggtgtagg agctcgccac caccaccgcg ccctcgtcgt agaagagctt ccagaactcg
    15181 cggaagctgg tccagttcgg cggaccctcg acgaccaggc ggtacttctc ttccttcatg
    15241 tcgccttcgg gcgtgatcgg gcccttgccc tcgcggatgc gctgctcgat ctccttgcgc
    15301 accaggtcgt agtactcgac cgcgtcggtg gtgccgcgga aggcggtgaa catcgggccg
    15361 atgtaataca cgccgccgaa gtaggcatcg atcggcgagg gacggttctt cgcgctctcc
    15421 agcacccaga ccaggttgtc ctcggccttg gccgagttct tcaggtattc gcgcagacgg
    15481 tcgatgtcga acttgacgcc cgagaccttc tcgaacatcg ggatgacttc ttccttgagc
    15541 tgcttgacca cgaagtcacg catgttcttg gtgatcttgc cgtcgccctg atacgggatc
    15601 tgcagcatca ccgtcggaca cttgtactcg tggcgcagca gctcgaacca cttcatgaag
    15661 gtgaagcagc cggtgtagga cagcagcagc atgtccggcg cgggcatcgg cttgccgttg
    15721 ggcgcgatgt tgccgcggcc catcatgccg atgtcggcct tgacgtaggt gcacacgtcc
    15781 tcggagtggc ccgccttctc ggcgtccatg atcatgccgc cggaaacctt gcgcatgccg
    15841 ttctggatgg cgttggtctc gggcaggttg ttgaccatgt cgaagcacat gatcagctcg
    15901 ttcaggttgc ccgggacgaa ggtcgacacg accttctcgc ccgactcctt ggtgccggtg
    15961 agacgatcgt agttgccggc gatcatctcc ttctgcttca ccatcgaggg ttctttgatg
    16021 acttcggggg aggtggctgc gctcatgatt agctccagag tttgatggag tcggcgaagg
    16081 tgccggcctg ctcgcggatc ggctgcatct ggcccgagtt ttccgcgtac ttgaacgaga
    16141 tgtaggggac gttgtgctcg gagcagcggt cggccagcat cggccgctcg agcagggccg
    16201 ggtcgcagaa gctgggcgcg gcgaagatga caccctccgc ggcgttgctg cgaacctgct
    16261 cgagcaggta cttgcccttg tcctcttcct tgtcgtcgta cttcgcggcg gtggcgatcg
    16321 actcgtgcag gaaggcgagc gacaggttct gcatcggatc accgccggtc gagaccggat
    16381 tgcgcagcca gcgatgcacg atcatgtagt cgtcatcgac gatgtagcag ccggcgagct
    16441 cgatcgactt gatcaggttg agcggcggct gctcgcagaa cgagccgttg atgaccacgc
    16501 ggcagttgtc gcgcttggat gccttcacat tctcggctgc ggcgaggtat tcgctcagca
    16561 tctggttgtg ctcttccacc ggcagcacga ggccggcgcg catcagcaga tagacttcgg
    16621 acgagggcac cttccacggc tcgcgggaac gcatgccgta caccgcctgc accagcctgc
    16681 ggttctcgtt gtagaccgcg atcgaggcac gcagcgcgtc gtcggtgatc ttgcgcccgg
    16741 agaggtgctc gaggccttca cgcagttcgt tcagctcgtt ggtgtagtag ctgccaccga
    16801 cgtcgtcgcg atagttctgc ggcacgtcga agtagcgcac gtacttgccc gggaacagca
    16861 gcttccacat gccggacagg ttgcggatca cgtcgcagat gctgggaaac agcatgccgt
    16921 cgacgaaatc catgcgcttg gacagcccca gctcgatggt cgagcgtggg atgcgacaga
    16981 tgtagctctg gtagtaggcg tcgccgtgga tcacctccag gccgtcgcct ccgcccatga
    17041 tgccgagcgg gagcatgccc gcggcgtgga tgatctcgcg cggcacatac accggcatgt
    17101 aggcgattac cttgcgcgag gggtcggcct ccttccactt ccttgcggcg gtgaaatcga
    17161 ggtcttcgta gagcgcttca cagcgcgcga tgatgtctgc cgtactcatg gtcaacggtt
    17221 ctcccagttt gcagaacgct tctcaagaaa ggcccgtaga ccttccaccg catcgtggct
    17281 ggccatcagc tcctcgagat acatggcctc cacggtttcc agtttttgct tgatcgccgg
    17341 cacccggtcg cagcgtgcgg cccgaaccgc gaaacgcagc gagctggcgc tcagcgcagc
    17401 aagatgttcg tcgtaccact tcagcgcggc cgcctcgggg tcgtcggcca gcacgtcggc
    17461 caggccgatg cggaacgcct cggcgccgtc gatgctgcgc cccgagaaca gcagctcctc
    17521 ggcacgcgat tgcccgacgc gcggcggcag caggcaggag gccgcaggcg cgaacacgcc
    17581 gaggcggatc tccggctgcc ccaggcgcgc atccggcgcc acgaagagca ggttgcccgc
    17641 cgccgccacc tcgagcccgc cgccgaggca ctggccgcgg atcgcgacca ggatcggcac
    17701 cgggctgtcg agcatctgcc gcaccagcgc atgcagggtc ttcagcatct gcgcgcactg
    17761 ctcgggcatg tgctcgtcca cactggcgcc gaagctgaag tgcgggccct cggcatcgag
    17821 cagcaccgcg cgcagcgcgg gcgaaccgag gtgggcgccg agcgcctgct gcagcgcgcc
    17881 gatcatcgcg gcatcgacga tgttcgcctt gggccgcgcc aggcgcaggc ggagcaggcc
    17941 gccgtcgcgc tccagccagg ccttgagcgg gttgatcgcc tcgcccatca cttcgccccc
    18001 ggcatcaggc tttcgatcag ctcgggcgtc cagggcgtgc cgacggcgag cgcctggcgg
    18061 aggtcggtga actcgatctc gcggccggtc tccttggtac cctcgttgaa ggcgcggaag
    18121 ccggtgcggg cctcgttcat catgttgagc gcgagccagg cgcggctgtt ctccttgttg
    18181 cggttccagg catcgagctt gggcttgcgc agctcctcga agctcttggt caggcactcg
    18241 gggaaggtgg agatcagctt ggcacacagc ttctcgaccg cctcgtcgag cagggaaagg
    18301 tcgatctcgc cgcgcttgag cagctccttg ccggcggcca gttcgtcgcc ggtcttgaac
    18361 tcgccgtgga tgatgcggcc gaactcgtcg aggtagcggt cggtgatgac cagcgggttg
    18421 gcgacgaact tgccatcgat cttgagcgcg gggacgatct gcgaggaaat gccgaggcgg
    18481 taggccttgt gcgccgagaa cggctcgcac agggtgccgg acaccatcgc ctgctcgcag
    18541 ccgatcatca ccggcaggaa gtcggtcgca ccgccgatcg cggccgagcc gtgcttgggg
    18601 ccggcctggc cgaagttggc caggtcctgg gcgacggtga agtccgccgc catgccgatc
    18661 tcctggccgc caccgatgcg catgccattg acgcggcaca ccaccggctt gtcgcagccc
    18721 aggatcgccg acaccatgtc gttgaaaaga cgcatgtact ggcggtattc ctgcgggttg
    18781 ccggcgtagt actcggcgta ttccttggtg ttgccgccgg tgcagaaggc cttgtcgccg
    18841 accgcggtga acaccaccgt ggcgacgtcg cgggcgcagg acgcggcacg gaaggcaagg
    18901 atcaggccct tcaccatgtc ggtggtgtag gagttgtact gcttggggtt gtccagccag
    18961 atccaggcgt tgtacaggcc cggcaccacc tcgccctgga gattgcgcgc ggggcgcttc
    19021 tcgtagatca cgcccggcac caccgtctcg ggcaccaggt tatggtcgac caagtgcttg
    19081 ggcgcagtct gatcgatcac gcgttgcgtc gtttctttca tctcgtatgg ctcctctgtc
    19141 tgctctcttg tcggattggt agggggtgcg ggatcagggg gtcaggatcg cgcggcggct
    19201 gagcttgtgc gcgtgagccg cctcgaagac ctcgttgatc tgcgcgagcg ggtggcgctc
    19261 gacgaaattg gcgaggttga tgcggccgtc gagcaccagg tcgagcgcgg ccgggtagta
    19321 ctcgggcagg cagccccagt tgccgagcgc gcgggcatgg aaggccatca ggttggagag
    19381 gcgaacctcg accttgtcca tcgtgaagcc gaccaccgcg agcgtcgcgc cgtgattgat
    19441 caggctatag gcgttggtct ggccggcacc gctgcccgag cattcgaaga tcttccagcg
    19501 cgtggtgcgc aggccgttgg ccttggcgtg cgcctcgatc gccttcttga ggtcgcgtcc
    19561 ggtcacctcg cgcgcattga gcgtcagcgc cgcaccgtac ttcgacatca attcgagctt
    19621 ggccggatcg acgtcgatcg ccaccacgct cgcaccgaag gcgttggcga tctgcaccgc
    19681 gtagccgccg acgccgccca ccccgatcac gatcgcgacg tcgccaggct cgactccggc
    19741 ctgcgtgacc gcctggtagg gcgtggtgac cgcgtcggcc accaccgaca cgtcggcgag
    19801 ttgcagtccg gcggcggcga ggcgcttctc atcgaccgcg cacaggccgc gcgccggcac
    19861 cacgatatgg gaggcgaagc cgccctggat gtcgttcccg ggcatcacct gggcgcggca
    19921 gatggtgccg tgtcccgacg tgcagagctc gcaggtcccg cacggcatca ccgccggcac
    19981 gaccaccgcg cggccgaccc actgccccgc cgcgctgccc gcgcccacca cccggccgct
    20041 gatctcgtgg ccgagcgcga gcggcagcgg ctggttggtg cgcacgccgt cgtagtagta
    20101 gccgagatcg gtgtggcaga cgccgcagcc ggcgacctcg accaccacct ggtcggcggc
    20161 cagctcgccg atctcgaact cggtgcggac catcggccga cccggctcca ccatcatcca
    20221 gcgatacggt ttggtgctca tctacccact ccttccgttt acgttcgcgt caactttgat
    20281 cgatctgatt ggcgactgga tcgactgctt cctgccgccg cgttggatgc aaagttaaat
    20341 caggccggca ggagggcagt tgaccaaagt caacagattg acgtttgcgt caacgtaaac
    20401 ttcgacacat cggaggtgag cgctgcgaag gcggcgctgc aacccaacca gcctccggtc
    20461 ggcgccgtgc ccgcttcacc cgagggggac gcgcgcatgg gccctgaaca ggaccgcgcc
    20521 ggcacccgcc ggtcgaaggt cgaacattgg agaacgagga tgacagcccg atcggtatcg
    20581 ataacattcg ccggcagcgg cggcgcgggg gtgatgaccg caggcaacat gctgctcgac
    20641 gccgccggcc gtgccggctg gtatgcctac atgacccgct cgtcgggcgc gcagatccgc
    20701 ggcggcgagg ccgccgcgat gatgcgtctg tctacgtcgc cggtgcagtc gcacgacgat
    20761 caatatgatc tgctggtcgc catcgactgg gagaacgtcg ggcgcttctc cgccgagatc
    20821 ccgatgaccg ccgatagcct ggtcgtcggc gatcccgacg gcggcgaatt ccccgaggcc
    20881 atccgcgcca agggcacgcg gtcggcagac atcccgttca agaagatggc caagacgatc
    20941 gagggcgggc gccccaacat gatcgccctg ggcgcggtcg ccggcctcgt cggccttccc
    21001 gaggacgccg tgctcggtgt ggtccgcgac tcgctcgcca agaagggaga ggccgcgcgc
    21061 accgccagcg aggcatcggt gcgtgcaggc atggccttcg cggccgacct gccgccctgc
    21121 ccccgcctgg ccaccgccca gggccagagc gagcgcctgt ggagcatcac cggcaacgag
    21181 gccgccggtc tcggcgcggt gcgcggcggc atccgcttcg tcgccgccta tccgatcacc
    21241 ccgggcaccg aggtgctgga gtggctcgca cccaacctcg ccaagctcgg cggcgtgctg
    21301 gtgcaggccg aggacgagct cgcctcgatc aaccagatca tcggcgcttc ctacgccggc
    21361 gtgccctcgc tgaccgccac ctccggcccc ggcctggcgc tgatgaccga atcgctcggc
    21421 ctcgcggtcg cctcggagac cccgatcacc gtggtcaacg tgatgcgcgg cggcccctcc
    21481 accggcatcc cggtgaaatc cgagcagagc gacctcaaca tcgcgctcta cggcctgcac
    21541 ggcgacgccc cgcacctggt ggtggcgccc aactcgctcg ccgactgcgc cttcgccacg
    21601 cagtgggcgg tccatctcgc cgataccctg cagaccgccg cgatcgtgct ctccgaccag
    21661 tccctcggcc agagccgggc gacgatctcg ccgcccgccg atcccggctt gcgcgcggtc
    21721 cgcctgatgc ccgagggcga ggcggccgaa cgctaccgcc gctacaccaa caccgcgtcg
    21781 ggcgtctcgc cgatggccgt acccggcatg aagggctacc agtacaccgc cgacggcctc
    21841 gagcacaacg agttcggcac gccgtccagc ggcgcggccg accacagcgc ccagctcgac
    21901 aagcgtctgc gcaagctcgc cctgcacgac tacggcacgc actgggccga catcgagggc
    21961 gacggcgaca tcgccgtgct gacctggggg tccaccaccg ggccggtgcg cgaggcgctc
    22021 gagcgcttcc gcgccagcgg cgggcgtgcc cgcctggtgt cgatccgcct gatctcgccg
    22081 gtgcgcccgg aacagctcgc cgccgcgctc gcgggcgtgg cgcgcgtgct cgtggtcgag
    22141 cagagccatg gcgcccagtt ccaccgctac ctgcgcgccc actacgacct cccggggagc
    22201 gtgcgcgcct tccaccggcc cggcccgctg ccgatccgtc cgaacgaaat cttccgccaa
    22261 ctggccgact ggagctgaac atggaagcca catccacctg cccgagctac tccgcgcgcg
    22321 actacaagtc cgaggtcaag cccgtctggt gtccgggctg cggcgattac tcggtgctcg
    22381 gctcgatcac caaggcgctc gccgagctgt cgatcccgcc cgagcgcgtc gccctcgttt
    22441 ccggcatcgg ctgctcctcg cgcctgcccg cctacaccaa cgtgttcggc ttccacggcg
    22501 tgcatggccg cgccctgccg atcgccaccg gcatcaaggt atcccgcccc gagctcaccg
    22561 tgatcgcctc cggcggcgac ggcgacggct tctcgatcgg cggcaaccac ttcatgcacg
    22621 cctgccggcg caacgtcgac atgacctaca tcgtcatgga caacgaggtc tacggcatga
    22681 ccaagggcca ggcctcgccg accaccgcgc ccgactggga gaagagcaag ctcaccccgc
    22741 agggcaccgg catcaacccc ttccacccgc tggtgatcgc actcgccgcg ggcgcgaact
    22801 tcatcgcccg cggcttctcc ggcgaccaga acggcaccgc gaagctcatc accgaggcga
    22861 tccgccatcc cggcttctcc ttcgtgcagc tgctcagccc ctgcgtcacc ttccgtcccg
    22921 agcagcgcga ctggaaggac atggtgcatc ccgccgcggt cgacccgacc gacgaccccg
    22981 cgcgcgccgc gcgccgcctg atgtccgacg atggcttcaa cgtcggcgtg ctcttccgcg
    23041 gcagccgcaa gcccttccag cccgggcagc cggcgagcgt cgattcagtt gcagatctcg
    23101 agaaggagtt cgtgttatga gcaaacccga tccgcaggaa accggcatcc actcggtgga
    23161 gcacctgctg gcccatgcgc tggcgatgga agtcgaggcg gtcgagcgct acgaggcact
    23221 cgccgaccag atggagaccc acaacaaccg cgacgtcgcc gtgctcttcc gcaagctggc
    23281 cgagatcgag aagctgcacg tcgacaacgt caacgacatc agcgagggac acaccctgcc
    23341 ccacatcgca ccgtgggaat actcctggga agcccccgag agcccggagg cgccgtcggt
    23401 gtcggcggaa ggcctgcact acatgatgca tccctaccag gcgatcgcga tggcgctcga
    23461 ggccgagcgc cgcggggtgg acttctacga ccgcctcgtc gcccagtccg agcgcgagga
    23521 catccgcaag ctcgccgccg aactgtgcga gaccgagcgc gagcacgtca ccctgctcga
    23581 aggctggctc gcccgcttcc agcccccgcc caagggctgg aacgaggacc ccgatccgcc
    23641 cttgctgcag ggttgaggag caaggggtaa ggcgccagac atgaggcgcc aggagcgatg
    23701 cgcgcgctgg gagaccagcg cgcgcatcgg cttttggagg tcgggcgaaa gatgcctgcg
    23761 gagaggatga agggaaatcc cgagcgaagg atctcgagcc gcgcccgtcc gccgtcgacc
    23821 gacgacactg cgaccggatg tgacgaggcg ccaggccgag aggtcttcac ccccccaggc
    23881 ctgacgcctc acatctcaca tctcacatct cacgccgtct gatcgacgct cagctcgtag
    23941 cccgcctcgc gcaggccctg cacgaccgcg ccggcatcct tggcctcgaa gcggatggtc
    24001 gcggtgcgca agccctcgga ctgcggcgtg cgcggcgaca ccaccgagac gatgtagccg
    24061 ccctgcgcat tgaccgaggc gagcagcgcg gcgagctggc cggcgcgatc ctgcaacttc
    24121 acggtgatcc gcgtcgcacc ctcgacccag cagccggtga tgtcgaggaa gaaatcgagc
    24181 aggtccacgc tggtgacgat gcccaccagg cgcccggcgt cgtccaccac gacgaggcag
    24241 cccagcttct tgggccgcat catgcgcgcg gcctcctcga tctgcgtgcg tggcgagcag
    24301 ctcaccaccg acttgtgcat gatgtccgcc gcggtgatct tggacagcag gtacttcacc
    24361 tcgccgacgt cgagcgtcgt gatcatggac ggctgggcgc gctgcacgtc gcgatgcgag
    24421 atgatgccca gcaccttgcg atcctggtcg atcaccggca cgtggcgcac cttcttgagc
    24481 cgcatgatct cggagacctg cgagaaagag gcctccggac cgacgtgcag cacctcccgg
    24541 gtcatgatgt tttcgacgaa catgggtcac tccagttgga agaagaaaca gcggtaaaca
    24601 gcgggttcga gacagcacaa gaggaatagc gggcacgcga cgcggccccg gccggacacg
    24661 gaccagcgcg catgcggtcg ccggatctgc ggctggaacg cagtttatcg gaaacccaca
    24721 tccccaagca gcgcctatag ttaacgctaa cgttaactct acacaaaaaa attacgttca
    24781 cgtcaacgtt actgtttgac aaatatcaac cgaccggtca gtcataagtt ctaggattca
    24841 tcccataagc caagaagggg gagcgggcgg cctcagcctc gcccgcacac caccggagga
    24901 gacacctgat gaccgtcagc agcccacagg gcacaaccaa ggtcgccacc tactgttacc
    24961 agtgcgtggc ggggccggac ctgctcaagg tgaaggtcga ggacggcgtg gcgaccgccg
    25021 tcgagccgaa cttcgacgcc gaggacgtgc atccggccgc gggccgggtc tgcgtgaagg
    25081 ccttcggcct cgtgcagaag acctacaacc cgaaccgcgt cctgcagccg atgaagcgca
    25141 ccaacccgaa gaaggggcgc cacgaggacc cgcgtttcgt gcccatctcc tgggatgagg
    25201 cgctcgacac catcgccgcc aagctgcgcg gcatccgcga gaccggcctg ctcgacgcct
    25261 cgggctaccc gcgcgtggcg gcgagcttcg gcggcggcgg cacgcccacc gcctacatgg
    25321 gcacctttcc cgccctgctg gcggcctggg gcccggtgga catgagcttc ggcagcggcc
    25381 agggcgtcaa gtgctaccac tccgagcacc tctacggcga gctctggcac cgcgccttca
    25441 tcgtctgccc ggacacgccg cgcagcaagt acatcctgtc cttcggcagc aacatcgagg
    25501 cctcgggcgg cgtatgcggc gtttggcgcc acgccgccgc acgcgtcgag cagggcgtca
    25561 agcgcgtgca ggtcgagccg cacctgtcgg tcaccggcgg ttgctccgcc gagtggctgc
    25621 cgatcaagcc caagaccgac gccgcctgca tgcacgcgat gatccacgtg atgctgttcg
    25681 agaacgcacg ctcgcggctg gacatcgatt tcctcaagca catgacggcg tcgccctacc
    25741 tggtggcgcc caatggcttc tacctgcgcg atcccgacac ccgcaagccg ctggtgtggg
    25801 acctcaagtc cggcaaggcc gtcgtcttcg acacccccgg catcgatccc gcgctcgacg
    25861 gcgacttcct cgcctccggc ctcgaggtgc tgcccgacca ggaactcgtc acccatgagg
    25921 ccgcgccggt gcgcagtgcc ttcggcaagc tgctcgacca cgagcgcacc ttcaccccgg
    25981 agtgggcgca gggcatctgc gacgtaccgg cggcgaccat ccgccgcatc gccaacgaat
    26041 acctcgacca tgccgagatc ggcgcgacga tcgagatcga gggccgcacc ctgccctatc
    26101 gcccggtggc ggtcacgctc ggcaagacgg tgaataacgg ctggggcggc tacgactgct
    26161 gctgggcgcg cacgctgatg gcctgcctgg tcggcgcgct cgacgtgccc ggcggcacca
    26221 tcggcaccgc ggtgcgcctg aaccgcccag ccaacgaccg ccagagcagc gccaagccgg
    26281 gcgtcgacgg gttcatggac tatccgttca acccgaccga caaggaaaac tggatctcgc
    26341 gcccgcagat ccgcaacgcc aaccgcaccc tggtgccgct ggtggccaac tcggcgtgga
    26401 gcgccgcgct cggtcccacc cacctggcct ggatgcagca gcgtcacggc ttcgagagct
    26461 tccccgagcc cacccagccc gacgtctggt tcttctaccg caccaacccg gtgatctcgt
    26521 tctgggacac gccccaggtc gccgaggcgg tggcgaagtt ccccttcgtg gtcgccttca
    26581 cctacacccg cgacgagacc aaccacttcg ccgacatcct gctgcccgac tgcaccgacc
    26641 tcgaaggcct gcagatgctg cgcatcggtg gcaccaagta cgtcgagcag ttctgggacc
    26701 accagggctt cgcgctgcgc caaccggtcg tgccgacgca gggcgacacc cgcgacttca
    26761 cctggatcag caccgaactg gcccgccgcg cgggcatcct cgagccgtac aacaaggcga
    26821 tcaaccgcgg cgccgcaggc gtgccgctga aggggccgaa ctacgatttc tcgctgcagc
    26881 ccgaccacgc ctacggggtc gaggagatct gggacgcgag ctgccgcgcg gccagcgccg
    26941 agctcaccga aggcgccgag gaccacgggc tggagtggtg gaaggagcac ggcttccgca
    27001 ccatcgagta tccgcgcctg cagtggtatc tctatcccca cctggtcgac caggggctgc
    27061 gcttcgagat gccctaccag gagcgcatct accgcatcgg cgaggaactc ggccgccgcc
    27121 tgcacgaatc cggcatcgac tggtgggacc gccagctcac cgaataccgc ccgctgcccg
    27181 agttccacga cttctcccac ctcatcaagc acgcggtgat ctccaacctg ggcgggcgcg
    27241 acgaggactt cccgttctgg ctgctcaccg cgcgcagcat gcagtactcc tggggcggca
    27301 acgtcagcct gcagatggtg cgcgaggtgg ccgccaacgt gaagggccac cgcggcgtga
    27361 tcatgaaccc gacggccgca cgcaagctcg gcatcgagga cggcgacctc gtcgaggtgc
    27421 gctcgccgct gcgcgagacc cgcggtcgcg tcgtgctgcg ccagggcatc cgccccgaca
    27481 ccctgctgat ggtgggtcag ttcgaccact ggatcacgcc ctacgcgaag gacttcgacg
    27541 tccccagcat gaacgccctg gtgccgatgc tgatggacct gaccgacgcc accggttcgg
    27601 ccgccgacat cgtgccggtg tcgatcaagc gcatcggagg tgcccaatga cccgctacgc
    27661 catggtggtc gacctgcgcc gctgcgtcgg ctgccagacc tgcaccgccg cctgcaagca
    27721 caccaacgcc accccgccgg gcgtgcaatg gcgctgggtg ctcgacgtcg aggcgggcga
    27781 attccccgac gtcacccgca gcttcgtccc ggtgggatgc cagcactgcg acgagccgcc
    27841 gtgcgagacg gtgtgcccca ccaccgccac caagaagcgc gccgacggtc tggtcaccat
    27901 cgactacgac ctgtgcatcg gctgcgccta ctgttcggtc gcctgtccgt acaacgcacg
    27961 ctacaaggtc acccgcgaca cccccgccta tggcgagggc aagatggcca acgaggtgaa
    28021 gcgtaccgac ccggcgcgca tcggcgtggc caccaagtgc accttctgct ccgaccgcat
    28081 cgactacggc cacgcccacg gcctcgtgcc cggcgtcgat cccgacgcca ccccggcctg
    28141 cgccaacgcc tgcatcgcca gcgcgctcac cttcggcgac atcgacgacc ccgacagcaa
    28201 ggcctcgcgc ctgctgcgcg agaacgagca tttccgcatg cacgaggagc tcggcaccgg
    28261 ccccggcttc ttctatctct gggagaaaaa gcaatgagag gcgtcgaacc cgaactgcag
    28321 cccttctggg acgaacgtgc cgcgggcaac ttcatcggcg gcggcaccgg cgccggactg
    28381 ctgctgtgga gcgcgatcgc ggccagcggc cacggcaccg cgatcggcct gccgctcttc
    28441 ctcgcgctcg ccttcatcgg cgtcggcctg ttctgcgtgt ggatggagat cggtcgtccg
    28501 ctgcgcgcgc tcaacgtctt cttccacgcg cgcacctcgt ggatgacccg cgaggcgatc
    28561 gtcgccggcc cgctcttcct gtgcggcctc atcgcgctgc tcagcggctg gttgttcttc
    28621 gcctggctcg ccgccctgat cggcctggtc ttcctgtact gccaggcgcg catcctgcag
    28681 gccgcgcgcg gcatcccggc ctggcgcgag ccggcgctgc tgccgctgat cgtggtcacc
    28741 gggctcgccg aaggcgcggg catgctcgcg ctgctctcgc cgctgctcgt ccgctcgccc
    28801 ggcgccgagt tcgccttccc gctgctggtg ctggtcgtgc tgcgcttctg gctgtcgcgg
    28861 cgctacgccg cccatctcgc ccgcccgaac aacgcccccc tgaagacgct gcgcgcgctg
    28921 cgcgagaacg atcgccactt cctcctcctc ggccacaccc tgccggcggc ggtgctgctg
    28981 atcgcgctct tcgccggcgg cagcttcggc accgcactgc tcgcgctcgc aggcatcgcg
    29041 gtcgccgcca gcggctggct gttcaagctc gcgctcatca cccgcatcgc ctacacccag
    29101 ggtttcgcgc tgcagcacgt tcccgcgcgc acccccggct attcgcaccc cggcgtcaag
    29161 ccgggctgga actgaacccc tccctccacc ccggggcggc ggaccatgcc ggaccgccgc
    29221 cgaaatcagg agcaaagcgc atgtacgagg tcaggatcca tggtcgtggc gggcagggcg
    29281 cggtgcttgc ggcccagctg ctcgcgaccg cactcgtgtt cgacggcaag tacgccgtct
    29341 ccattcccgc cttcggcttc gagcgccgcg gcgcgccggt ggtgtccttc atccgcgcga
    29401 gcgaacagcc gatccgccag ctcaccaaca tctacgcacc cgactgcatc gtctgcgtcg
    29461 atcccgcgct cgcccgcacc gtgaacatct tcgccggcct caagcgcggc ggcaccctgg
    29521 tgcaggcgac ctcgcgcccg ctcgccgaga tcgacgtgcc cgacagcgtc ggcaccgtcg
    29581 gcctgtgcga cgcggtgcgc atcgccaccg agatcttcgg ccgcccgatc accaacaccc
    29641 tgatgctcgg cgccttcgcg cgcagcaccg gccaggtctc gctcgaggcg ctgaagaagg
    29701 tgatcgagca atccgatttc cgcgacgccg gcctgcggca gaacctcgac gccctcgagc
    29761 gcggctatgc ggaaacgacc gtgcacaccc tcgaacggag gatcacagca tgagcgaacg
    29821 ccaccaacac tacccggcct ggcacctcaa ggaggccggg gttcccgacg acctctgtcc
    29881 ggtcgccacc gagcagagtc cgatgttgcc gggcgactgg cgcagcttcc gcccggtcgt
    29941 cgatcgcgac aagtgcgtca agtgcgcggt gtgctggctc tactgtccgg tgcagtgcgt
    30001 cgaggagcac gccgcctggt tcgacttcga catgaagacc tgcaagggct gcggcatttg
    30061 cgcgcacgaa tgcccgcagc gggcgatcac catgattccg gaggcgcaat gaacaccacc
    30121 acgctcgaca ccgctcccac caccgaaaag cgcccggcac cgcagctgat gctgtgcgag
    30181 ggcaacgagg ccgccgcgct cggcgtcgcg ctcgcccgcc ccgacatggt cgcggtgtac
    30241 ccgatcacgc cgcaaacctc gctggtcgag aagctcgcca agctcatcgc cgacggcagg
    30301 atggacgccg acatcgtcga tgccgagggc gagcattcgg tgctgtcggt gctgcagggc
    30361 ggcgcgctcg ccggcgcacg cacctacacc gcgacctgcg gccccggcct cgccttcatg
    30421 ttcgagccct acttccgcac ctcgggcatg cgcctgccgc tggtgatgac catcgtcacc
    30481 cgcgacggca tcaccccgca gtcggtgtgg ggtggccacc aggacgcgat gacggtgcgc
    30541 gaggcgggct ggatccagat gtactgcgag accgtccagg aagtcctcga caccacggtg
    30601 atggccttcc gcctcgccga gcatcacgac gtgatgctgc cggtgaacgt ctgcctcgac
    30661 ggcaactacc tctcctatgg cacctcgcgc atcgagatgc ccgatcaggc cgaggtggac
    30721 gacttcatgg gacacaagga cgtcaactgg cacgtcgccc tcgacccgct gcgtccgatg
    30781 gcggtcgacc cgctcaccgg cggctcgggc ggcaacggtc cggagacctt cgtgcgctac
    30841 cgccgcggcc agtgcgccgg catgaagaac gcactgcacg tgatcaccga gatgcacgag
    30901 gactgggcgc gccgcttcgg cgaagcccac cgcttcgcac cgctggtcga ggaataccgg
    30961 ctcgacgacg ccgagtacgc aatcatgacc ctgggcagca tgaccggcgc cgccaaggac
    31021 gcggtcgacg aggcccgcgc ggccggcgag aaggtgggcc tcatcaagat caagaccttc
    31081 agccccttcc cggtcgaggc gctgcagcac gcgttgcgcg gcgtgcgtgc gctcggcgtg
    31141 gtggaccgct cggtcaattt ccgctggaac tgcggcccga tgttccagga aaccctcggc
    31201 gtgctctacc gcctcggccg ccaggtgccg gcgcaaagct tcatcggcgg cctggccggc
    31261 gccgacctca ccgtcaccac cttccgccgc gtcatcgacg agacccgcgc cctgctcgag
    31321 cgagcgccct ccgacgagcc ggtgtggctc aacgccaaag actgagcgcc aaggaccgag
    31381 gaaaccgcca tgcacaccac cgactacctg atcgccggca gcagccacgc cgcgctcgag
    31441 gccatcaacg cgatccgcat gcacgacggc gacggcagca tcaccgtcgt cacccgtgat
    31501 ccgcaccgcc cctactcgcc caccgtgctg ccctacgtgg tctcggggcg ctcgacgccc
    31561 gaacgcgtgt tcctgcgcga cgacgacttc ttcaccgcga acaaggtcga ttaccgcagc
    31621 ggctgcgcgc tcgccggcct cgaccccgcg gcgcatcagg cgcgcctggc cgacggcagc
    31681 accatcggct accgcaagct gctcctcgcc accggcgcca gcccgatcgt gccgcccatc
    31741 cccggcatcg agcgcgtcga ctaccacgtg ctgcgcacgc tcgacgacgc cacccggctg
    31801 cgtgcggcga tggccgcgtc gaagaacgcg gtcgtgctcg gtgccggcct ggtcggcatg
    31861 cacgccgccg aaaacctggt caaggccggg gccagcgtca ccatcgtcga gatgagcaag
    31921 cagctcacct ccggctactt cgacgaggtc gccgccggca tgatcgagca ggccttcctc
    31981 ggcaacggcg cgaagatccg caccggccac cgcgtcgtcg gcctcgagcc gagcgccgac
    32041 ggcgccaccc tgaagctcga caacggcgac accatcccca ccgacctgct gctggtcgcg
    32101 gtcggcgtgc gccccgagct cggttatctc gacggcagcg gcatcgccac cgagcgcggc
    32161 atcctggtcg acgacaccat gcgcacgagc gcagaggacg tctggtccgc cggcgactgt
    32221 gcgcaggcgc gcggcttctt caccggcacg ccggtgatga acgcgatcct gcccgacgcc
    32281 acgctccagg gccgcatcgc cggcatggcg atggcggaag atcccgggct caagccctac
    32341 gcgggcggtc tgccgctcaa cacctaccac ttcttcggcc gccacgcgat ctcggtgggc
    32401 agcgccgcgg tccccgaggg cgggcgcgcg caggtgcgct tcgacgccgc gagcgggcgc
    32461 tacctgcgcg cggtgttcga ccgcgacgag cgcctgaccg gcatcttcgg ggtgaatgaa
    32521 ttcttcgatg ccggcgtgat ggcccagctc atcctgcgcc gcaccgatct cggaccgctg
    32581 cgcgaacgct tcttcgcgca gccgctcgcc accggccgcg agctcatgtc gcagctctgg
    32641 cgctaagggg aagatcatgg gacaagccta ctccaccatc gccttcgacc ccgccaagtg
    32701 cgacggctgc ggcaaatgca tgacggcgtg cgcgagcgtc aagttcgact ccaccgacac
    32761 cacgcgctcg ctgatccaga tcgtgcgcag cggcggcgcc cgcatcgagc cgccccgccc
    32821 gggcgagttc gagctcgcgc tgtgccgcca gtgcgccgat cccaagtgcg tcaccgtgtg
    32881 cccggcgggc gcgctcggca agaacggcac caccggcgtg atcgactggg acgcctcgaa
    32941 gtgcgtcgac tgcctgctgt gcaccatcgg ctgcacctac gccggcatcg cgctcgacga
    33001 gcacagcggc cgtgtcagca agtgcgacac ctgcgagggc aagccggcct gcgtgcccgc
    33061 ctgccccacc ggcgcgctgc gccacatcac caccgcgcgc atctacaacg aggtcggcag
    33121 ctgggaagac ctgttcgcac ccggcctggc cggctgccag ggctgcaaca ccgagctgct
    33181 gatgcgccac gcgctgcgcc gcgtcggccc cgacaccgtg ctcgccaccc cgccgggctg
    33241 cgtgcccggc atgggctcgg tgggcttcaa cggcaccacc ggcaccaagg tcccggtctt
    33301 ccacccgctg ctcaccaaca ccgcgtcgat gctcgccggg gtcaagcgcc agtacgagcg
    33361 cgtcggccgc aaggtcaccg cgatggcgat cgccggcgac ggcggcgcct cggacgtcgg
    33421 cttccagtcg ctctcgggcg cggccgagcg cggcgagcgc atcctcttca tggtggtcga
    33481 caacgagggc tacatgaaca ccggcatgca gcgctcgagc tgcacgccct acggcgcatg
    33541 gacctcgacc accccgatcg gcgcacatgg ccgcggcaag acgcaagacg ccaagaacct
    33601 gccgatgatc atggtgaacc accgctgcga atacgtcgcc accgcgtcga ccgcgtacat
    33661 ggaggacctc tacgagaagc tcgacaaggc cctcgccgcc tccgagcggg gcttcgccta
    33721 cctgcacgtg tattcgccct gcaccaccgg ctggcgcttc cagagcgcgc tcaacatcga
    33781 ggtcgcgcgc aaggcggtgg agtcgaactt cgtcaccctg tgggaataca cccccgaggc
    33841 cgggctgcac ttcacccgcc cggtggacaa gccgctgccg gtggccgact acctcaaggc
    33901 catggggcgc ttccgccacc tcagcccgga gcaggtcgag cacatccaga acaaggtggc
    33961 cgagaacctg cgcttcgtgc gcaagatcgc ggagcaggcc gatgagcaat gagaagccgt
    34021 cccccccgga gcagatgagc cgcgccgagc tgcaggcgct gcagctcgag cgcatgcgct
    34081 ggtcgctgca gcacgcctgg gacaacgtgc cgcactaccg gcacgccttc gaggccgccg
    34141 gcgtgcggcc atccgacctg cgcggcctgg ccgacctggc gaggttcccc ttcaccgcca
    34201 agcaggacct gcgcgacaac tatccctacg gcctcttcgc ggtgccgatg gccaagatcg
    34261 tgcgcgtgca tgcctcgtcc ggcaccaccg gccgccccac cgtggtcggc tacaccaagg
    34321 gcgacatcga gcgctgggcc gcggtcatgg cgcgctcgct gcgcgtggcc ggcgcgagct
    34381 gcgaggacat catcctcaac tcctacggct acggcctctt caccggcggc ctcggcgccc
    34441 actacggtgg cgaggcgctc ggcgccacgg tgatcccgat gggcggcggc aacaccgaga
    34501 agcagatcca gctcatccag gagttccgcc ccacggtgat catggccacg ccgtcctaca
    34561 tgctgacact ggccgacacg atgcagcgca tggggctcga cccctcggcg accacgctgc
    34621 ggctgggcat gttcggcgcc gagccgtgga ccaaccagat gcgcgccgag atcgagcgcg
    34681 tgctgggcat cgacgcgatc gacgtgtatg gcctgtccga agtgatgggc ccgggggtgg
    34741 cctgcgagtt cgtcggtcac aaggacggcc cgcacatctg ggaagaccac ttctatcccg
    34801 agatcatcga cccggtcacc ggcgaggtgc tgcccgacgg cgagcgtggc gagctggtgt
    34861 tcacctcgct caccaaggaa gcgatgccgg tgatccgcta ccgcacccgc gatctcaccc
    34921 gcctgctgcc cggcagcggc tgcgcgatgc gccgcatcga caagatcacc gggcgcagcg
    34981 acgacatgct gatcatccgc ggcgtcaacg tcttcccgac ccagatcgag gaggtgctgc
    35041 tgcgccacga caagctgtgc ggccactacc agctcgtgct cacccgcgag ggtcacctcg
    35101 accagctcac cgtgcgcgcc gagctgcgcc ccgagctcgc cgacggcatc ggccagggcc
    35161 tgcgccgcca gatcggcgag gaggtccggc acgcgatcaa gagcttcgtc ggcatcagcg
    35221 ccgagatcga gatcctcgac cccgacagca tcgagcgcac ccaggtcggc aaggcaaagc
    35281 gcgtgatcga ccgccgcaac gccgcaacca gcaccgcctg agggcgcgcc gggaacgcat
    35341 caccccacaa gaacagacac ggagacaccc gatgaccgaa gtccgaagcc atgccgccag
    35401 ccgcgcagca ggggaggccg cgcagcgcta ccgcacggcc ctcgcaaccg ggcgcgcgac
    35461 gctggacgcc gacgagctcg cccgcctgct cgccgccgca gacctgcaca ccacgaccgc
    35521 gccagcggac gcgatcgaac tgtcgatccg cgtccatgcc acgcgcgagt tcggcctggt
    35581 gctcagcgcc ggcgccggcg gcctcgacgg cgcgctcgac cccgccaact tcgcgcgcga
    35641 ccgcgcggca gtgcatgccg cggtcgagct caccgacggc gaggacttcc tcgagcgttt
    35701 ccgtcgcacg atcgcctggc agcgcatcac cgccctcgcc gcccgtcgcg gcgtgcagcc
    35761 cccggacgcg gcgctcgccc gcctcttcga ggccgcgctg cagctggccg cgggcggcct
    35821 gcccgacgcc ccgggcgcgc aggccgcgct gcaggagctg gcgctggact gcgcctgcga
    35881 cggcgaggct gtccgggttg tcgcggcacg ctgcagcgtc ggcgcccctc cgccgctgcg
    35941 cgtggcacga ccaatccaca agatcgaccg cctgctccac cccgagcgca tcggcatcgt
    36001 cggcgcttcg gccagcggca tgaacttcgg tcgcatcatc ctgcgcaacc tgctcggcag
    36061 cggctgcgcg cccgagcgcc tgtgcgtgat ccgccccggc ggcggcgaga tcgacggcgt
    36121 cgcctgcatc gagaacctcg ccgcgatcga gggcaagctc gacctgctga tcgtcgcggt
    36181 cgccgccgac gcggtctacc ccctggtcga cgagatcatc gcggccggca ccgtcgaggc
    36241 cgtcatgctg atcccgggcg gcctcggcga gaccgcgaag agccgcgcgc ccgccgccga
    36301 cctggcggcc cgcatcaacg ccgcgcacgc gtgcggcgac ggcgggccga tcttcctcgg
    36361 tgccaactgc ctcggcgtgg tctcgcaccc ggggcgctac gactcctggt tcattccgct
    36421 cgagcgcctg cccaagccgc agaaggcacc cgaacgccgc gcggtcatgc tgagccagag
    36481 cggcgccttc atgatcaccc ggctctcgca caacccctgg ctcgacccgc gctacatgat
    36541 cgcgatgggc aaccagaccg acctcaccca cggcgacctg ctgtcctggt tcgccgcgcg
    36601 cgaggacgtc gacagcatcg gcatctacgt cgaaggcttc cgcgacctcg acggcctggc
    36661 cttcgcgcgc gcggtgcggc gcgcggtgct cgccggcaag caggtcgtgg tgtacaagtc
    36721 gggccgcagc gaggccggcc aggccggcgt gatgggccac accgcctcga tcgcgggcga
    36781 cccggtgctg ttcgcctcgg tgctcgccca ggccggcgcc accgtcaccg acgacgtcga
    36841 ggtcttcgac gacctcttct acatggccgg caccctgcac gccaagcgca tcggcggcga
    36901 ccgcctcgcc gcggcgagcg gcgccggctt cgaggcggtg agcgccgcgg acgcgatcgt
    36961 cggcgagacc ttctcgatgc gcatggccgc gctgtccgcc gacaccgtcg gccgcatcga
    37021 ggcggtgctg gccgagaaga agctcgccgc cctggtcgag gtgcgcaacc cgctcgacat
    37081 gaacccgggc gccgacgacg acgcccacgt gcgcatcacc gccgcgatga tcgaggatcc
    37141 cgaggtcgac gcggtcgtgg tcggcctcga ccccaccgcc cccatggtgc gcgcgctcga
    37201 acagagcagg ctgcgccccg gctacgacct gtcggacccc gagagcacgg tgcacaccct
    37261 gccgccgctg gtggcgcgca cagacaagcc gatcgtcgcg gtcgtcgacg ccggccgcct
    37321 ctacgacgcc atggccgccg gcctgatgga tcgcggcgta tgcgtgttcc gcaacgtcgc
    37381 gcgcggcacc cgcgcgctgg tgcgccacgt cgaggcccgc ctggacgccg agcgtatccg
    37441 ccgccatttc ggccaatcgt gattccagcc cgcaagcacc ccgcagcacg aacacacacc
    37501 caaccacccg cagacggaga cagacgatga gcgacaccct tcaacccggc ctcagcctca
    37561 cccgcaagta cgatatcgac gccgcccgca cgatcgcctt catgggcgac gagtgccgcg
    37621 tctattccac ccccgcgctg ctctacgaca tcgaggtgtg ctgccgggac ctgctgctcc
    37681 agcacatcgg agaaggcaag gactcggtcg gcacccgcgt cgagctcgac cacgtcggcg
    37741 ccaccctgct cggcatgtgg atcgagatca ccgtcaagct ggtccaggtc gatggccctg
    37801 cggtggtgtt cgagttcagc gcgcgcgacg ccctcgagga ggtcgcccgc ggccgccaca
    37861 accgcttcgt ggtcggcgtc gacaagaccc gccagcgcct gctcggcaag ctcgccaagg
    37921 cgcaggcggc ctgagcgggc cagcgttcgc ggcttgccgc actcctgcac ggcggaagcg
    37981 gttctccgta ggagcggcgc gagccgcgat acggcgcatg gatcccgcgt catcgccgcc
    38041 acaagccacc attcgcgcct tgcggcgctc ctgcacggcg gaagcggttc tccgtaggag
    38101 cggcgcgagc cgcgatacgg cgcctgaatt gcgcgcccac ccgtttcagc gcaccaggcc
    38161 agcgcgcgaa aaatgcagca caccaccggc cgtcgccacc gcggcggtca gcgacaggcg
    38221 atccgcccgc cgctccaccg cgaagctcgc cccgccatcg cggctgtcga ggctgacacc
    38281 gtcgaggccg accgcatgca cgcgaccgtc gaaggcgacg aggtcggtga ccgagctctg
    38341 cgagccggtc tcgacgcgct gccagtgttc gccgttgtcg gtgctgcgat agacgctgcc
    38401 gcgcaggcca ccgacgatca gcgtgccgtc ggcgagcgcg gtgccgctcc agaacgtgcc
    38461 gcgatagccg gtcggatgat agctccaggt cgcgccgcca tcgagcgagc gcaacaccag
    38521 gccgcgctcc ccggcgatga atagtcgccc ctgcccatcg ccgaacacgc tcatcaggtt
    38581 ggcgtcggcg cgcgtgtcgc ccggcggcgg cggcagctcc gccgcctccc aggtccggcc
    38641 gccgtcgcgg gtgcgcagca tcagcgacca cagcccgacc gcgactccgt tgcgtgcgtc
    38701 ggtgaacgcc accgcgaaca gcggcctgtc ctcgacaggg tcgatgcgct gcacgctcca
    38761 ggtctcgccg ccgtcggcgc tgtgcaggat cacgccgaga tgccccaccg cccagccctc
    38821 gcgcgcgtcg gcgaagctca ccgcggtcag ggtcgcgtcg accggcacct tgcccgcctg
    38881 gcgccagtgc gcgccgtcgt cgtcggagag cagcaccacg ccgcgctccc ccaccgcgac
    38941 gaggcggggg cccgcacggg tcgcgtcgag gatcatgctg cgttcggcga aggccgagcg
    39001 caccgcaggc cgcggtacga aggcggcggc agcggcggcc gcgccgtgca ccgacgtatc
    39061 gccggcagcg ggcgccaggc tcgcgcagag cgccgcgcag caggccgcga tgcgggtcgc
    39121 gaagaagcgc acgcggcgcg gcgtcgagaa ggcgttcatg tccactccgc agtcagtgcg
    39181 ccaggatgcc cggcgcacgc accggcccct tgcgcgggaa aaggcgttcc agccacaccg
    39241 cgaaggccgg cagcgcagtc atcgccatca ccatgttcac catgaacatg aaggcgagca
    39301 gcttgcccat gtcggcctgg aacttgagct ccgagaacat ccaggtcgcc acgcccgccg
    39361 cgagcgtgat cgcggtgaag atggtcgcgg tgccgacctc gaggatggag tgttccagcg
    39421 ccttgacgat gttctcgccg cgggcgaggt gaagctgcag gcggttgtag atgtagtagg
    39481 cgtaatcgac gccgatgccg accgccagca ccatcaccgg cagggtcgcc accgtaaggc
    39541 cgatctcgag ctccttcatg aaccagtagc cgatgaaggt gcccacggtg agcggcaggc
    39601 agcacgccac cacggcacgg aagtcgcggt acatcagcgc caccagcacc atgatcgccg
    39661 cgtacacata gagcatcatc ggcagctcgg acttgtgcac ctcttcgttc accgcggcct
    39721 gcacgccggc gttgccggag gccagccgga tcgtcagccc gggctcgcgg tcgcgctcgc
    39781 ggaaggcctt caccgccgcg atcacgcggt tgatcgtggt cgccttgtga tcggacagga
    39841 acaggtgcac cgcggtcatg ctgcagtcct tgttcatgaa gccgcgcacg cggccgatct
    39901 cggtggcgag cgcggcgtag ttgccggggt cgcccggtac cacggacatc ttcgggttgc
    39961 cctcgttgta gccctcgttg taggtgcgca gctggccgga gaaggacagc gccgacagca
    40021 cgcccggcac atactgaatc tcggcgacga agcggtcctg atagcgcccg accgcgacgt
    40081 cgtcgcaggc gtcgggcggc gcctcgaaga tgaccgtgag ccagtccagg ccggtgtcgt
    40141 agctggaggc gatcgacacc gcgtcgcggt tgaagcgcgc ctccgcacgc agctccgggg
    40201 cgcccggctg caccgtgccg atgacgcggt cgtggctctg ccacaccgcc accgcgaaca
    40261 ccgccagggt cgccaggatc acgatcgccg cgttgcgcgg ctcggccacg cgcgccagcg
    40321 tgcgcagcca ggccgagcgc tgctcgcgct tgagcacggc cttgtcggcg tattccttgg
    40381 tgaaggtgaa cagcgaggcc gccagcggca gcatcaccag gttggtgacg atcttgtagc
    40441 cgacgccgag cgaggcggtg atcgccagct cgcgcaccat cgggatcggg atcaggatca
    40501 gcgtgatgaa ggacacgaag gcggtgacca gcgccagcgt gcccgggatc agcagcccgg
    40561 tgaagctggc gcgcgcggcc tcgtcgctcg atttgccgtg cgagagctcg cgcacgatga
    40621 agttgatctg ctgcacgccg tgcgagacgc cgatcgcgaa caccaggaag ggcaccagca
    40681 ccgcgagcgg gtcgagcccg tagccgagca gcctgagggt gccgaactgc cacaccagcg
    40741 aggtcagcga gcaggcgatc ggcagcaggg tgaagcgcac cgagtggcag taccagtaca
    40801 ccgcgagcgc ggtcagcagc agcgcgatcg cgcagaactc gagcaccgcc gaggcgccgt
    40861 cggcgatgtc gccgatctgc ttggcgaagc cgatgatctg gacctcgaac tgcgcgtcct
    40921 cgaatttcgc gcgcagctcc tcgaggatgc ggttgtaggc gacgtagtcg agcgccttgc
    40981 cctcgcggtc gcgctcgtgc agctcggcga cgatcatcgc gctggtctgg tcgcgcgcca
    41041 ccagggtgcc gatgaagccg ccctggctgg ccgcgcgctg gatgccggcg atgatctcgg
    41101 gcgtgaggtc gcgcggcatg atggtgcccg agatcagcgg atcggcgcga aagccctcct
    41161 cggtgatctc gttgacgaag gagttgggcg tccacagcga ctgcacgccg aggcggtcga
    41221 cgttgggcag gaagctcacc gcctgggtga cctcgtacag gcgttccagc ccggcctggg
    41281 tccagatgtc gccgctgcgc gccttcacga cgaagttcag ccggttggcg ccgagcacgt
    41341 cctcgcgata cttgtcgaag gtctggatgt actcgtggcc gatcggcatc tgcttctcga
    41401 agccggcgtc catgcgcagc tgcagggcaa agtagcccat cagcgcggtg aacaacatga
    41461 gcgcgaccag gatggtgccg cggaagcgga agcacaccgc ctcgagcagg cgcaggtagc
    41521 gggtcaggaa ggtgtcgacc tcgctgacgc tgaagggatt gagcatgggc gggtgctacc
    41581 gatcgggatt ggaacgggac agcgggccgg aggcggcgct cgccgccccc gggggtgctc
    41641 ctcagaagtt gcgcgagatg aagccgccga cgaagtcgcg atccttgtag ggcgcgctga
    41701 agctgtagtc gccgccccag aaccgggtgt agttgatgcc ggcctgccag gtcatcgggt
    41761 tgcggttgaa cagcacatag aagttggccg acttcgcccc ctccatccag ttggccatga
    41821 agttgggcgt gttgccattg accgcatgcg agaagaacac ccccgggatc acctgccagc
    41881 ccgggatcag ggtgctgtcg taggtcaggc tgaagtcgaa catgtagccc caggacagct
    41941 tgtcgccgac gccgctgtcg tgcgtccacg cgccggcggc ggggagttgc tggtaggtca
    42001 cgccgttgcg ggtgcgcacg taggccttgt ccttgctcac ccccggatag gcgatcgcca
    42061 ccgcctcgcc aaggaaggtg ccggtctgcg cgcccatcag gtcgaggaac cagccgtggt
    42121 cgccgggcgt caggctgagg atgccggtga ggtggaactg gtagcgctcc tcgtcgatcg
    42181 agccgtcgca ctgatcgggt gacccgaagc cgatgtcctg gcaggccccg agcgaaaccg
    42241 catcctccgg ccgatacgac aactcggcgc ccaccgccca gttgcccacc gaggtgttgg
    42301 cgctgatgcc gtagagctcg cggtcctcca gaaagtacat gcgagccgca ccgttcgcgt
    42361 tccagtattg aaggttgggg gtcttgtcgt ggaagcgctg gtagtagagg ccgagatcga
    42421 cgtccatgcc ctcgggccgg tacttcagcg agacgccgaa ctggccggag tcgcccggct
    42481 cgtcgtcggc gtcgaccggg atcgccgtga gttcactgtt cagcatttct tcccgagccg
    42541 cggcgagggt cttgccgtaa agcggatcac ccggcgtacg aatccggctg gcggcatccg
    42601 gcgtgaaata gatcaggctg tcacgcccct tgtcgaagaa atcgctctgc gagaaatacg
    42661 tccctgccgg cgggaagctg tagggcttcc actggaactg gtagtaggcc tcgacgttca
    42721 ctccgctgcc gaggttggcg gcgaccgaca gcgccggcga gggcaggaag gcctccttca
    42781 gctgcacgcc gggcgaggac aggcgctgca ggtccatcgc cacgttggag ttgatgccgc
    42841 cgatcatgaa caggctctcg ccccagctca ccacctggtt gccgaagcgc acgcggccgc
    42901 ggcgctcgcc gaggtcgaat tccttgctca cccacaggtc gtagaggcgc acgtactggc
    42961 cgaccgcctg cttggcgtcg ccgtcgagcg gggtgcgcgc ggtgtcgtcg gcgacgaagt
    43021 cgtacagcca gcccacccgg cccatgaact tccactcgtc gggaaagctc agcagcagct
    43081 cgtgcgagcc cttgaggtgg gtggtgaaca ggtcgccctt gtcgtagttg agattgccgt
    43141 cgtcgccgtt gccccatacc gcggtgttgg catcggcgcc gcagggggtg gattgatcgc
    43201 cgtggtggct gcactcgcgg ccctccacgc gcttgccgaa gcccgcggtc agcgtggaat
    43261 cgagcgaacc gcgcaccgag ccggcctcga actggaaggc gtacgccgcc ggcgcacaga
    43321 tgccggccat ggccagcccc agcaccagtt tcttcagatg tgtcgtcatg atcgttctcc
    43381 gcaggacgcc ccgcccgggc cgcatgacgg cccggcgcac gcgcccgggt gatggtcggg
    43441 aagaaagagg agggagcggg ctcagcggtc gctgatcgcg cgcaggttgt ccggggtgta
    43501 gaaggcgtcc ttgtacttcg gatcgtcggc ctcgaccgcc cagaccatgt ccttgccggc
    43561 acccatgccc gcctggtcga agagcacacg gccggagacg aggtcgtact gcacgaaggc
    43621 cgggttgtcg caggcgcccg tttcggcgac cggaatcagg aagctctcgc ggaccttcca
    43681 cagcttgccc tgggcgtcgt agtcctccgc gcccatcagc gcccaggagt cctcgtcgaa
    43741 gtagaagcgg cgcttgggcg cgacgtggcg cacgccgtcc ttggcggtgg cctcgaccac
    43801 ccagacgcgg tgcagctcgt agcgggtcgc cttcggatcc acgccgtcgg gcgtcaccac
    43861 cttgcggcgg tcggcagcgg ggtcgtacat gccgaagctg ccgtagccga tgaacatttc
    43921 cttcttgccc accagcttcc agtcgaagcg gtcgggcgtg ccgttgaaga cgcgcggctc
    43981 atcgagcgtg tactggttct cgaagccgac ctggggggcg tcgtaggagt agctcggcat
    44041 gcggcgcacg cggcgctggc cggggaagta gtagaaggtg tcgctggtct tgttcaggta
    44101 cgaggtgatc accagcgcct ggccggccag tgcggtgggc gaggtgtagt tgaagtaggt
    44161 gtggtactcg accggcggca gctcgctgag cttgttcgag cctttcttgc cccaggggta
    44221 gtagtaggtc tgcgtggacc cggcctcgat ccagtcgctg ccgccgctgc gcggcgacag
    44281 catcacccac agcgccttca tgtcgagggc gacgccggcg tacttcatct tggcgttcca
    44341 cagcacctcg atgccgctct gcggcagcgg gaagggaatg ccgggcacgg tggcctcggc
    44401 caggctccag ccgtcggcgc cgatcttcgc catgcctacg ttggccttgg tgttggcctg
    44461 cacgaagtcg ggcgcggagc agtagcggcg gctcggatag acctcgagct tgtagttctt
    44521 gaagcccttg atcaccgcga cctggccctc ggtgagcttg ccggcgtatt tgtcgacgtt
    44581 ggcggcatcg atcgtaaaca gcggcttgtc gcccttgtgc ttgaagtagt cggcgcgcgg
    44641 cttgccgtag ctccagcccg gctcgagtgc cgagccttcc gtccaggccg ggatgctgcc
    44701 gtcggcgttg cccgcgcgtt cgccgccgag cggggtcagc gtggtgccca ggctcgccgc
    44761 atcggcggcc agggcggcgt ggctggccag cagggcgccg gcgagcgcag tcagcttgca
    44821 gaacctcatg agtgtctcct ccacgtttct gattttgtcg gcagcggcag gcggtcaccg
    44881 aggacgccac cactgcgcag cggccgaccg ggcccgcctt cccgccagcc ttgcaggcct
    44941 atggtgtgtc gagcatagcg acgcggtgta aaccgcattt ccgcgaaatg tgccgatttg
    45001 tgcgcagttg tagcggaggc caccacggcc ctgccccacc ggcccgcctt cctcagcgca
    45061 gcgtcttcag gtactcgatc accgcccggc gctggaccga gtccttcagg cccgcatagg
    45121 ccatcttggt cccgggcgcg taggcctgcg gatcggcgag ccagcggtcc agctccgcct
    45181 ccgtccacac cccctcgcgc ttccggagtg cgcgggaaaa aacgaaaccc ggggtgcccg
    45241 cgatcgcccg tccgaagcga ccgtgcaggt tgggccccac gccgtggggc tgccctgcag
    45301 cggcgggatg gcaggcggcg cactggacga agccggactc gccgatgtcg gcggcaagcg
    45361 ccggtgccgg tacggcgagc acgccgagga tcgcgaccac ggcgcgcagc gggacggatc
    45421 tgctcatgat gccggctccc atcacgcgcg accgcgcagc tcggccgcga ccgcctgtcc
    45481 gacctgcagg ccggactcga tcgcgccgtc gatgaagccg cgccagccgg tggcgatgtc
    45541 cgccccggcg aagaacaccc ggccttcgcg ggcacgcagg tcctccaggt gcttgccgaa
    45601 ctgccctgga cgcagcgtgc accaggtgcc gcgcgaatac ggatccagcc cccagtcgta
    45661 gccgagcacc tgttcgaccg caaggtgggg caggaagcgg cgcacgaagg gctcgaccgc
    45721 cttcgggtcg ttgagatcga ccctgccgtc gactggcgga ccgaaggcga ccagcacgcc
    45781 gccgtcctcg cccggctggt cggtgaacac catcgtgaac agctcggtct cgggcgcgaa
    45841 gaagatcagc gtctccagcc ggcccttcac cttcagatag agcttgtgcc ccttgcccgc
    45901 atggtgctcc ttcgccatcg cggtcttgcc cggccgcagc ggcggcgaga actcgatctg
    45961 gccggccgtg ttcaccggca ccgcgacgac cgcgaagcgg gcggagatgc gttcgtccgc
    46021 ctccgtcacc aggctcacgc ctgcggcgtc ctgactcagc tccgccaccg cggtcgacag
    46081 gcgcacgtcg aagccgccgt cctcggccat gcgctcgatc agcgtcgagg tcccgtcctt
    46141 gagcttgtag cgcgcgcagc tgtagagcag ccgcgccgca tcgccatcga ccagcgacca
    46201 ccagcgcagc atctcggtgt aggcgccgct cgcgatcggg ccgtgacagt tggtggccat
    46261 catcgcgttc atgaggtcgc gctgcgccgg actcatgtcc attgccgcca tgcggtcggc
    46321 gatggagagg tgatcgagct tgcgcccggc ggccgacagg cccgccatga acggtcgttc
    46381 gaagacctcg cccgcctcgg catggaagcg cttcagcgcg tcctcgagca gcgcccagtt
    46441 ctccatcacc gggatctcca gcaccttgcc ctcggacatc cacagcacgc ggtcgggatg
    46501 ggcgacgccc ggcgtctcgg cgatctccat gccgtagcgc atcacctcgg cccacacgtg
    46561 gggctgggtg tagtggatcc aggtgccgcc cagttccacc ttgcgctcgc cgaagttcgc
    46621 atggaaggtg cgcccgccga gacgattgcg cgcctcgagc agcagcaccc tcgcgccacg
    46681 gtgggcgagt tcgcgcgcgg cggtgacgcc ggcgaagccg ccgcccacca ccaccacgtc
    46741 gtagccgggc tgcgcgggcg ccgccgcgcc gacgcccacc ggcaacaccg aagcggccgc
    46801 agccgtcgat gccagcttca ggaaccgtcg ccgcgcactg tcgtcggcgc ccgcggccga
    46861 cgccgcagag gtcttgtatt ccatgatctc cgatctcctc cgtcgccgct gtccatgctc
    46921 tggaatgagc gtccgcggcg tgttgcgctc gcaccagctg gccgagtcgt gatgtggaga
    46981 ttaggaagac cgatgcatgg gacggtatcc gcaatcggca tggaaatgcc gacgcagtgc
    47041 agcaaacgct caaccgggac cgtgcggcgt gctgccaccc tctcgccgac gcgtctccga
    47101 gggcagctct ccgaacaggg cccggtagta cgccgcgaac cgcggcatgt gccagaagcc
    47161 ccagcgcgcc gcgatcgcgg cgatcggttc atcggggctg ccggtcgcaa cacggagctc
    47221 gcgccgcacg gcgttcaggc gcacgttgcg caggaactcc accgccccca tcccgacgag
    47281 gtcctcgaac gcgtactgca gcgagcgccg cgacaccgcg aggctgcggc agatgtcggc
    47341 gatctggagg ggctcgctcg ggcgctcgag cgccagctcg cgcaccttgc gcacgatcca
    47401 ggccttcacg ctcggacgca ggtccgccgt cgggcgctcc gaggcccgat ccagcgtatc
    47461 gagcatcagc gacaggacgc agtcgcgcat cgccgccctg ctcgccggaa tctccagcag
    47521 gccgggctgg cggcgggcca cgacgacgat ctcgatcagc gcgcggcgca acatcccggc
    47581 acgcctcggc tgctgctgga gaaacgggcg ctgcagcgat gcctccgcgg tccgcggcga
    47641 gtcgctggcg gcaaatccgc acagcaggga ttcggacacc gtcgccgaga cgatatccat
    47701 gcgctcgcgg gagaagatct cgaaatctcc gccgcccgga agccacgaca ccgccccgtc
    47761 ctggatcgga acgccgcaat acatgccggc gccgccgcct gccaccggaa tgccgagcga
    47821 caccatgccc gcccgattgc tgcctgcggt ccacaccgag cggttgcccg tctcccacag
    47881 gacctcgagg ccgtcgtcga tccacaactc ctccgcgccg ccctcgaagc agcccggtgc
    47941 gagctgctcg taggtcgcat tccaggcaag cagccccctg ccgaaatcgc tcacgtcgcc
    48001 cacctggaca ggcccgggca ggcccgccga taccggggcc gcatcgggtg ccgcgggcgg
    48061 caagggcgag caagcgggtg tgggcatcgg cgctcaccag gcggtggggc gaacagacgg
    48121 tggcggggcg ggatgaggtg cgccggcggg ccgcgtcccc acgcctgccg cgcatcggca
    48181 agcgatccgc gcgccctcgt cctggcgcgg gccgccaggt ttgcccgcgc cgcgcgctcc
    48241 gcctgttaga atttcacgct tgttcaaccc cgagtcgagc gaccgatggg ccgtggcaac
    48301 gacgaaacga gcgcgcagac cccgccggag ggcatccgct tcgggatgct ccccgagctg
    48361 atcggctacc gcctgcgcat cgcgcaaatc ggcatcttcc gcgacttctg cacgagcatg
    48421 gacgaagccg agatcacccc caccctgttc ggcgtgctgg tgttgatcga tgccaatccg
    48481 ggcatgaagc agacccagct cgcagacgcg atccagctcg accgctccag cgtggtgtcg
    48541 gtgctcgaca agctcgaggc acgcggcctg gtcgagcgcg tgcgcgcacc ggacaaccgg
    48601 cgcgccaacg ccctgcatct caccgctacc ggccggcact tgctgcagcg cctgattccg
    48661 caggtccgga cccacgagga acgcgtgctc caggatctcg atcctgccga acgcgtgcag
    48721 ttgcgcaagc tgctcggccg cctgctgctc gcggccggct gaaacggccg gccgcgcatg
    48781 cggactcagt tcgccgtgcg caccgccacg gtgtaatccc cgacgccgct ggcgcgattc
    48841 cagtgacgca cctgcacgaa atactcgcct tcgaccagat ccgtcgcgat gcgcgcgttg
    48901 ctgcccaggc cggagtcgtc gtcctccgcg atcagcgcgg tcggtgaatc ggggccgaag
    48961 agtttcatgt acacgtcggt cggaccgcgc gtgtcgacca cgtagcgccc gggctgcgtc
    49021 acgcggaagc ggaatacgtc ctcctcgccg aacttgccga tgctggcggc ggtgcgccgg
    49081 cgtgcgttga ccttcagctc ggtcacctcg cccgttacgc cgggcttggc gcgcggatac
    49141 atcttcgcgc cggcgatgaa ctgcttgtcg agctccgaca gcacctcgtt gcgcgaggtc
    49201 gccacgccgt tgagggtcca ctccgcaggg aagaagtaca gcatcaccga cttggggtcg
    49261 aagtcggtgc cgttgacctg gtttgcggcg tacttgcgca ggatgttgtg ccgggtctgg
    49321 gtctcgctcc agtgattggg cggccccgcc atggcgcgga tcaccgcctg ctcgttccac
    49381 tggatgccgc cggcggggtt ctggtgctcg tgcgcgagcc cgatcgcatg gccgaactcg
    49441 tgcgccgccg taccgccgtc gaggaagccg aggttcatcg tcggctcgtt gaccgggatg
    49501 ctcttcgcgt cggtgccgat gtagctccag gctccgtcgt tctcgtcgaa ggtgatgcgg
    49561 atctcggccg cgggggcgtc gtcgaacacg aactgcaggt tggcgtgcgc cgtccaccac
    49621 gcggcctgct cctgcacctt cttcttctgc tgcgcgcttc cacccatgaa acgcacatgc
    49681 agggtggagc cgttgatcca ggtcttgccg atcggcgata gtccgcgcac ctggcccggc
    49741 cggtcgggca tggtggtctg gaagcgcatc aggtcccggg gcagcacccg gtcgaagcac
    49801 accttgggcg ttcgggtcat ggcggatccc tccctgacgt ggccgcgact cgccgcagca
    49861 agcggcgttc gccgaatccg gtcgcggcag cggccggctc ctgctgaaca tatagtacgg
    49921 gcccccgtca ggcgccaggg aaacacgcac cgcgactacg ggcgccgcca ggccacgcgc
    49981 acgtcggcgg tcgacatgtc gcccgggagc tggatcgtgc cgatcttctc cagcgtgccg
    50041 ctgtcgtgga tgctgatcgt gctgcccgcc ccgccgacgt aaacacgcga ggcgtcgcgc
    50101 gacagttgca ggctgtagta ggtgtggtcg agctccacca ccttcagcgt ctcgcgcttt
    50161 tcgatgtcgt gcctggagag cgtattgaag gcgccgtaga ggatgttgcg atcgcgcgga
    50221 tcggcgacaa gcgagaagat cacgaactcg aaggggaaga tctcgcgcgc ctcggccttg
    50281 ccggtcgcga ggtccacccg gctcatgccc caccaccatt cggcgctcgc catgtcctgg
    50341 cggtcgtcgg cgaacttcgc ggtcacgtag ggcatcacgt actcgttcag atgctcgccc
    50401 tgcgagtgca tcgcgaacgc gtccggcgtc agccagcgcg gaccgcggtc ccagctcgcc
    50461 agcgccgaca cggtgcgcac cgcgccgctg cgcacgtcga tcgcctccac gtcggcaccg
    50521 ccgaggatca cctcgccgcc ggcggtggcg gcgatcttgg tgatgcggcg cttgaccggg
    50581 aagctgcgca ccggcttggc gtccagcccg tcggcgatgc gatataccgc gaggcgcggc
    50641 ggcagcacct cgaagcggtc ggcaccgcgg cgcaccgggt tctgcacggt gtacagctcg
    50701 ctgccgtcgg cgctcaccgc gagcgactgc accgtcttga cggtgacgtc gccttccgac
    50761 tggcgggcgg agaacaccag cttgcaggtc tcgagctcga agccgtacac gtcctcccac
    50821 aggttgccga gcacgaaggc gtgacggccg tcgcgcgtcg gcaccagcgc gcccgagccg
    50881 aacttgccgg gcagctcgca gctgcgcgcg atgcggtcgg tcttcatgtc gatgacgtgg
    50941 agcaggccgg ggcgggtgac ggtgagcagg tactccgcgt cgctcgtcgc ggatttctgc
    51001 gccggggtcg cgcacgcggt cgcgagcgcc gcggcggcga tcgcgccggc tgcccgcagc
    51061 gcacgcaggt cggggatgag gctcatgagg atcgctccca ttcagatctt gtcctcgaag
    51121 atgccgtcca gcttgcgcca gtccctggtc gcatcggcct tgccgtcgcc ccagtcgggg
    51181 taatggctca tggtgtcggg cacctgcgcc ggccaccagc acgagtcggc gcaaccgtag
    51241 aggtcggcct ccatcggctg gcacagcgag gcggcgccgc cgaaggcgtc gatctcccag
    51301 cccgggtcgg tggtcgcggt gcagccgacc accgtctgca tggcgacgac ctcctcgatc
    51361 gcctcgggct tgccctccgc ggcgtccagg cgctgcgcct tcttgttgag cgacttcagg
    51421 tgcttcatgc ggtcctccgc ggggaaacgt gtgcggcgaa aaagccgggg ttctcggtga
    51481 cgatgcgcgc gtaggtctcg atgccgaagt cgatccagtc gcgcagcagt tcgcagtagt
    51541 gataggaagg cgcggcggca tcgccgtact tggcgtagct ctcgtggtag cagccgccgg
    51601 agcagaggtt gcggatgcgg caggtcgcgc agccggtgcc gttgcggtcc aggcggcggc
    51661 cgatgaaggc gcccaggcgg gcgcggtcga ttccggcatc gacgtcgccg aaggttggca
    51721 gcgcggagcc ggtgaagcga tggcagaggt tgagcccgcc cttgccatcg accgcgagca
    51781 gcccgacccc ggcgccgcag ggcagccgct tcttgttgcc ctcgtggagg tcggtcatga
    51841 gctggtgcag attgccgaag ccgaggctgc ggttgcgcag cgccgcgtcg agatagcgcc
    51901 gaccgagcgc cttgaggccg tcgaacacct cggcgagctc gtcggcggtc aggttgaagg
    51961 cctggatgtc gcccgaggtc accgggccga agccgacctc ggcgaagccg acctcgtggt
    52021 gcaggtgctc gaagatcggc accaccccgg tgatgccgcg ggtgagcgtg acccggcagc
    52081 cgaccgggcg cgcacggtag cgcgcaagca gcaggtcgac cttgcgccgc accacgtcgt
    52141 aggtaccctc gccgccgaag ctgcgccggt tgcggtcatg caccgcgggc ggcccgtcca
    52201 tcgacacggt gacccccacg cggtgggcgt ccagccagtc gatgatctcg gcggtgagca
    52261 gggtggcgtt ggtggtgagg gtgaaatcca cgcgcgcgcc gacctcggcg aagcggcgct
    52321 cggcgaagtc gaccaccgcg cggatcaggc ccatgttcga caggggctcg ccgccgaaga
    52381 agacgagggt gtatttcggc ttgccgggcg actcggcgag catcatctcg atcgccttcg
    52441 tcgccgtctc caggctcatg cgcaggccct tggccggcgc gctgaggtct tccttgtagc
    52501 aataggtgca gctcaggttg cagcccgtat tgacgttgag caccagggtg tcgaggggaa
    52561 agcgcgtcgg cgggtccggc ggcggctcga tcgggccgtc ctcgccgtgc tcggcgagca
    52621 cgtggagctg gcgcagctcg tccaccgtct cgcgcagggc gagcgcatcg tggcggccac
    52681 cgaggcgatc ggccagggtc gcggtctcga gcgtggcctg ctcgtcgaac agcgccagca
    52741 cgtcgcgcgc ggcgccatcg agctcgaaca ggctgctgct cggcacatgg aacagcagct
    52801 gccggcccgc gctgtcgacg cggtgcaggt tgggccggat cagggtcagg gtcgtcatcg
    52861 gatccgcccc gcgctcaatc gagcgcacgc tgaacgaagt cgggcaccgc gacgtcgagc
    52921 ttgccgcgcc cctcgaccgt cgcgcccgcg gcctcgaccg tggcgaccac attcacccgg
    52981 ccggtgttgc cgagcgattt cggccgcgcc gggttcggtc ccgcatcgcc cgggacgaac
    53041 acgccgtcgg caccgatatg gccgacgaag tccgcatcgt gcgccttctc ggcctcctcg
    53101 tcggcggcct cgatcgacca gcgtgcaggc aggtaaccga ggcgcaggtc gtcggcggta
    53161 cccgccttgc cgtcgcgccc cgccgcccag ccgatggcgc ggtaggcgac gcccaccttc
    53221 tgcatctgcg catcgcccgg cccgcccacg cgcgcgatcg cgttggccgg ctcgacctcg
    53281 acgcgcgcca cccgcccata aactgcgagc agccccttgc cgcgcgcgct gccgaccgcc
    53341 acgtcgttca gcccgtcacg gccctcggca tgcgcacgca ccaccacgcg gtcggcgctg
    53401 cgcgagacca cctcgagcac tcgcacgccc ttgccgagcg acaccgcccc cgccagcccg
    53461 ctgccgacca cggtcagctc ggcggtctcg ccgcggcgca gctgcgcggg catcaccgcc
    53521 atcaccatcg gcgcggcacc ctcgcgcagc gcgctcagcg ccccgcccat gcggcgcgaa
    53581 tcggcaaagt gctggcgccc atgcatgcgc tcgccgcgcg catcggccgc catcacctgg
    53641 cgcagcgcga cgccgtcgat gtcgacgctg ccccgccact cgtagccggt gtacaccgtg
    53701 gccacgccac gccccgagag cagggccccg tcggcgtagc ggccctcgat cgccagttcg
    53761 tagcggtcct cgccggccgc gctcgcgcgc atccgtccct cgaacgcgcc cttgccgggc
    53821 aggaagccgg cgatccgcca gctgccgtcc agcgccgtgc gcggcgcggc ctgccacgcc
    53881 ttccatgcgc gcgcatcgag cgcgaagtcg cgcgccagcg cgggggcgac ctcgttcacc
    53941 gcgagcggga accactgccg gtcgcgtgcc agcgagtgga actcgagcgt cgggttgagg
    54001 ccgatgtgcg tgtgcatcag cagcttccac tcgtcctcgc tgcgccgctg cagcgcgaag
    54061 cgggcgccgg agtggcagcg cgcgcacatg tcgcggtagc gcccgtcgat ggtctcgacg
    54121 cggttgttgt cgtgctcgag cagatagcgc cagcccgccg tctcggccgg ggccagcccg
    54181 cgcgtgtcgg ccaggtactt gatcaggcgg cgcttgtcct cggccgagac cttcgcgccg
    54241 tgctgttcct gcatccgggt gatcgtcatc tgccagcctt cgggggtctt gcgctggccc
    54301 tcgacgcggg aaatgctgcc gccctgcgcg ctgtggcagg acaggcagtt cgcggccagc
    54361 agcgccgcgc cgtccgccgc cagcgcccgc tccggcgcgc cgaccacggc cgccagggcg
    54421 agcagccccg ccgcgatcat cgacgtgatg ttcctttcct tttccaaggc cttcgctcct
    54481 cgctccgttg cgcatgcggc ccgggcgggt gccgtggcgg cccgccggac ttccgatgac
    54541 ggacacgata agtccgtccg gtaaacgaac ttggcggccg atgtgccgag ttgtgcgcga
    54601 atgtaaacgc cgtgcaacat cccacgcacg ccctctcgcg acggattgcc ggtgacgggg
    54661 attcggggga tcatccgcgc acaacaagcg aacgttcgac cccaaacgaa ggaggatcga
    54721 tgtcgaagga gacacccgcg atgccgcaca tcatggtggt cgaggacgac cccgtctcgc
    54781 gcgcattgct cgtcggctat ttcgagaagg aaggctatcg cgtctccgcg agcgacaatg
    54841 ccgacgacct gctgcagcgc gtcgagcagg agcgcatcgc gctcgtgctg ctcgacatcc
    54901 gcctgcccgg caaggacggg ctcacgctca cccgcgagct gcgcgccgcc tcgcgcgtcg
    54961 gcatcatcct ggtgaccgcg cgcgacgacg agatcgaccg catcgtcggg ctcgagctcg
    55021 gcgccgacga ctacgtcacc aagccgttca acccgcgcga gctgctggtg cgcgcgcgca
    55081 acctgctctg gcgggtggcg caatgcgccg gcagcgagac gcgcgaggcc gagcaaggct
    55141 gcttccgctt cgaaggctgg acgctgaacc cgcacaagcg ccagctggtc tccccggacg
    55201 gccgcatcga gcgcctgccc cagggcgagt tcaagctgct gatggccttg ctgagccacg
    55261 ccggccaggt gctgtcgcgc gaccagctga tggatgcgat ccacgaccgc gagtggacgc
    55321 ccaacgaccg cacggtcgac gtgctggtcg ggcgcgtgcg ccgcaagctc gacgacaacc
    55381 ccgccgaccc gcgcctgatc ctgaccgcgc acggcgcggg ctacctcttc gccggcgagc
    55441 tcgactgaac ccgcgccggc cgccgcgatg aacgcgcccc cgctgcaata ccgcctgctc
    55501 aagcgcctct accaggggcg ctgcagcacg gtgagccgcg tgctgctgcc gggacgcgac
    55561 gcgccggtga tcgtcaagca gctcgatcgc ggacgctgcg cgcccggggc gcccgcacgc
    55621 ctgcagcacg agttcgagct gctcggcacg ctcgagctgg aaggcattcc gcaggcgctc
    55681 gagcagctcg agttcgagcg cggcccggcg ctggtgttcg ccgaccgcga ggcgcattcg
    55741 ctgcgccagg cgatgcggcc cggcagccgc gactggcacg actggctggc gctgatgatc
    55801 cgggtgtgcg agctgctcgg ccggctgcac gaggcccgcg tcatccacaa gcagatcaat
    55861 ccggaccaca tcctggtgac cccgtccggc gcgcccctgc tggtcgattt cggcatggcg
    55921 acgcgcttcc tgcgcgagca ggcggactgg aacacgccgc agctggcggg acatcgcctg
    55981 ccctacctcg cccccgagca gagcgggcgg atcaaccgcg agatcgacta ccgcaccgac
    56041 tactacagct tcggcgccac cctgtacgag ctgctcaccg gccagccgcc cttcgcgggt
    56101 cgcgaagggc tcgaactggt gcattgccac atcgcccgcg agccgcgccc gccgcaccac
    56161 atcaaccgcc tgctccccga ggcggtctcc gccgtggtcc tgaagctgct cgccaaggac
    56221 gcctccgagc gctaccagtc ggcgcacggc atcgtgcacg acctgcgcga gtgcctgcag
    56281 cggcgggaac agccggcggc gcacgacttc gtgctcgcca gccgcgacgt ctccgcgcgc
    56341 ttccaggtgc ccaggcggct ctacggccgc acggcccagc tcgccgaact cgagggtcgg
    56401 gtcgaggcgt gcgccgccgg ggggcgcgcg atcgtgctga tcagcggcta caccggggtc
    56461 ggcaagtcca gcctggtgca cgagttgcgc cgtgccgtcc tcgagcgcca cggccacttc
    56521 gccagcggca agttcgacca gtaccgccgc aacccacccc actccgcgct gctgcaggcg
    56581 ctgcgcgagc tcgtccgtca gcatctcacc gagccgggcg aacgcctcgc ggccctcggc
    56641 agccgtctgc gcgagcagct cggcggctac ctcggcacgc tggtgcgcct gctgcccgaa
    56701 ctcggcctcg tcgtgcccga cgccggcacg tcgccggccg gccagcaccg cttcgacgag
    56761 cagggccgcc tgcatctgtt ctcgcgcctg atcgacgcgc tcaccgagcc cggacaggcg
    56821 ctcacgcttt tcctcgacga cctgcaatgg gccgacccgg cctcgctcgg actcatcgag
    56881 tcgctcgcga cccacgccgg gctgccgcgc ctgctgctcg tcggcagcta ccggcacaac
    56941 gagatcgggc cgggccatgc gctcgccggg ttcgtcgagc acctgcgcag gagcgcgctg
    57001 ccgccggtgg aactgcgcct gcagccgctc gacctcgcgc aggtccgcca gctgctcgcc
    57061 gacaccttgc gctgcagccc gatcgaatgc gcgcgcctgg ccgaagcctg ccacgagaag
    57121 acgcagggca atccgttctt cctcaaccag ctcctcgacg cggtgcacga ggaaggcctg
    57181 atccgcttcc acggccaacg ctgggaatgg gacgaagcgg cgatccgcgc gcgcgcgctc
    57241 agcaccgacg tggtcgagct catggtcgag cgcctgcgcc gcctgcccga acgcacgcgc
    57301 gcggtgctgc cgctggcggc ctgcatcggc ggcaccttcg acctcaagac cctgtccatc
    57361 gtcgccgagg ggcgcgcgcg ccagctcgcg cacgacctcg tgccggcgct cggcgagggc
    57421 ctcgtggtgc cgctcgacga cggcgaccgc cccgagcgcg accgtctcgg gcgcgcgcgc
    57481 tatcgcttcg cgcacgaccg cgtgcagcag acggcctact cgctgctcgc ggacgacgcg
    57541 cgcgagcagc tccatctgcg cgtcggccgc ctgctgcgcc agaacctcga cacgggcgcg
    57601 ctcgaccgcc gggtcttcga gatcgccggc cacctcaacc tcgcgcgcag cgccatcgac
    57661 gaccccgacg aacgcatcgc gctcgccacc ctcaacctgt gcgccgggct gcgcgcgcgc
    57721 gaatccgccg ccttcgagtc ggccctcgac tatctcgaca gcggcctcca cgcgctgccc
    57781 gcggacgcct ggcgcacgca gcaccggctg tgcctcgacc tgcacctcgc cgccgcggat
    57841 gcggccaaca gccgcggcga cggcgagcgc atgcaggccc tgctcgatgc cgcccaggag
    57901 cacgcgcacg ctccgctcga ccgcgtacgc tgcgagcaga tccgcatcca ggcgcacgtc
    57961 gcgcacaacc gcttcggcgc cgggctggag atcgcgcgcg acaccctcgc gcgtctgggc
    58021 gtacatcttc ccgcggcgcc cggccgcctg cgcatcgcgg cagaactgct gcgcacccgc
    58081 ctgctgatcc gccacaccgc gccggaggaa ctgctgcaca tgcccgcagc gaccgagcca
    58141 cgcgtgctcg ccgcgcaggc gctgctcgga ggcatgttcg gcatcgtcaa gttctccagc
    58201 tccgccctgc gcccgctggt gatggcgcgc caggtcgagc tcgcgctgcg ccacggccac
    58261 accgacgcca gcgcccaggc cctcgccggc ttcggcggcg tgctctgcgg cgtgctcggc
    58321 gcgatcgacg agggctaccg cgccggcacg ctggcactcg cactggaggc ggcgcgccct
    58381 tcgcgccagg ggcgccagcg caccttgtcg ctgttcaact gctacgtgcg ccactaccgc
    58441 gaaccgctgc agtgcgccct cgacggcctg cgcgaggcac acgccgaggc gctcgcctgc
    58501 ggcgatctcg agtacgccgc gtacagcctc gccgccgcga tccagtacgc ggtgccgctc
    58561 gccgacgatc tcgaggccct cgcccggcgc atgcgggggc agttggtgca gctcgaacag
    58621 accgggcagc agcagtccct gcaatacagc tacatggcgc ttcaggcggt cacccggctg
    58681 tgcgagccgt cggcctcgcc caccgcgctc gacggcgagc attaccgcga ggcggcgatg
    58741 ctcgaggcgc atcggcgcgg gaaccacctc accgcggtct gcctgcacca cttctacaag
    58801 gccttgctcg cgctggtgca tgcggactat cccgccgcac tcgccgaatg caccctgggc
    58861 gaagcccacc tgccctatgt cggcggcacc tataccgcga cctggttccg cagcctgcat
    58921 gcgctcgcgc tgctgcgcac cctgccgcca ccgggcaagg cgcgccggcg cgcgctgcac
    58981 acgctgcgac gcctggtgcg ccacgtgcgc atgcaggcga ggcatgcgcc cgccaaccac
    59041 gcccaccgcg tggcgctgat cgaggccgaa cgcctgcgcg tgcgcgtccg ccccggtgca
    59101 gcggcggcct acgatcgcgc catcgcgctc gcggagcagg gcggcttctg gctcgacgcc
    59161 gcgctcgcct gcgagttcgc cgcgcgcttc cacctcgatg ccggcgaacg cgcggtggcg
    59221 cgcagctgct tcgacgacgc ctgccgacgc taccagcgcc tcggcgccca ctcccccgtc
    59281 gtccatctgc acgagcagct cgaggaatac ggcctgtggc cccgaccgcc ggggctcgcg
    59341 ggcgcgggcg aaatgcaggg cggcctcgac gagaccaacc attccttcga cctctcctcg
    59401 gtcatcgagg cctcgcaagc gatttcggac gagatcgtgc tcgagcgcct gatcgagcgc
    59461 ctgctgcagc tcgcgctgac gagcgcgggc gcccagcgcg gcgcgctcgc cttgagccgc
    59521 gaccggcatc tgcgcgtcga ggcgatcgcc gggctggaag agggctcgac gctgatgtgc
    59581 agcctcgccc tcgaagccgc gacgagccgc gtgccggtca gcgtgctcaa ctacgtcgcc
    59641 cgcaccggcg aggcgctggt gctgggcgac gccccgcgcc agcagatgtt cgcgcacgac
    59701 ccctacatcc gcagccaggc gccgcgctcc ttgctgtgca tgcccatcct ctaccacggc
    59761 gagctcaccg ccctgctcta cctcgagcac cgcctcggcc gcgacatctt cgaccagcaa
    59821 cgcctgaaga cgctgcgcat cctcgccgcg caggcggcaa tctcgatcga gaacgccaag
    59881 ctctacctcg gcctgcagca atccgagcag gcctaccgct cgctgtacga gaacgcgatc
    59941 gagggcatct tccgggtcga tcccgacggg cgcttcctgt ccgccaaccc cgcgctggtc
    60001 cgcctcctcg gccacgacag cgcggcagag ttcctcaccg gcatcaccga cgtcagcacg
    60061 cagtgcttcg cccgcgccga ggaccagcgc cgcttcctcg gccgcctcaa catggaggag
    60121 aaggtggtcg gcttcgagac gcgctggcgc cggcgcgacg gcagcgaggt cgaggtctcc
    60181 atctcggccc gccgcgtgct cgacgacggc gggcggctgc gctgctacga gggctcgctc
    60241 accgacatcg gcgaacgcaa ggccaaggag cgcgccgaac tcgcacgcga gcgcgccgag
    60301 gccgccagcc gcgccaagag cgacttcctc gccgcgatga gccacgagat ccgcacgccg
    60361 atgaacggca tcctcggcat ggcgcagctg ctcgcgcgct ccgggctggc gccgggccag
    60421 gccgaatggg tggacgcgat ccgggcctcg ggcagcacgc tgctcgggat cctggacgac
    60481 gtgctcgact tcagcaagat cgaggccggg cagctcacgc tcgagtccgc gcccttctcg
    60541 ccggccgagg cgctctccgc cctgcgtccg atgctggcga cgatgagcgc acagaaaggg
    60601 ctagtgctgc gcctggagat cgatccggcg ctccccggag gggtgctcgg cgaccgtcgc
    60661 gcactgcacc agatcgtgct caacctggcc ggcaacgcgg tgaagttcac cgcgcgcggc
    60721 ttcgtcgcgg tgcgcgcgcg gtgcctgccg gcgcgcgcgg ggcgctgccg cctgggcctg
    60781 gaggtcgagg acagcggcat cggcatcgcc gaggaggcgc agcgccgcat cttcaccgaa
    60841 ttcacccagg ccgacggttc gatcacccgc cgcttcggcg gcaccggcct ggggctggcg
    60901 atctgtcggc gcctcgtcga cctgcagggt ggaagcctgg gcgtgcgcag ccgcccggga
    60961 gagggcagca ccttctcctg cgaactggaa taccccctcg caaccccggc gccacgggca
    61021 tcgccgcgtc cgcacgctgc catcggcgag gcgcgcctgc acgtgctggt ggtcgaggac
    61081 gtggaactga accgctgcat cgccggcacg ctgctcgagg acgagggcca caccgtgcgc
    61141 ttcgcgtccg acggctacgg cgcgctcgag ctgcacgagc gcgaggattt cgacacgatc
    61201 ctgctcgaca tccaccttcc cgacctcgac ggcatggagg tcgcacgccg catccgccgc
    61261 caccccgacg cgcgcaaggc gcgcgcgcgc atcgtcgcgc tcaccgcttc gatcaccacc
    61321 gacgaggtgg cgcgttaccg cgcggccggc gtggacgcgg tcgtcggcaa gcccttcgac
    61381 ttcgacgagc tgatccgcag cctgcgcgag ccccccgtac cgcccgccgc ggcaccgggg
    61441 agcgccctga tggacacgcg cctgctcgcc acccatttcg cccgcctcgg cgacgagcgc
    61501 gtgcggccga tgatggacat gctcgccacc cagggccgcg ccgggctcgc cgagttcgcc
    61561 gcgagcgccg accccgcgac ccaacgcgag cgcctgcacc ggctcgtcgg catggccgcg
    61621 aacctgggcc tcgccgcctt cgccacgcgt tgccgggagc tcgaggcgac ccccctcccg
    61681 gccgacctgc gcgcacaggc cggcgcgctg cggacgctgt tcgaggacag catcgcggcg
    61741 ctgcacgcgt tcgacccccg ggacgcgcaa gctgcgctcg aagccgcggc gcgctgaggc
    61801 cgcccgcctc gcgccgcgca cgctacaact gcgcacaatt ccgcacaaat cgtgaaaagc
    61861 tggtttacag cgccgggcta gtcttcccat cgacgcaggc agggccgcgg cagccgaccc
    61921 gcgagcccgt caccacgcct tccaactcct gccaggatcc gatccatgaa caagacgccc
    61981 tccctcgtca tcggcggccg caaggtcgcc ggcgaccgcg gcaccctgcc ggtcatcgac
    62041 cccgcgctcg gcgaggcctt cgccgagtgc ccgaacgcct cccccgcgca gctcgacgag
    62101 gcggtggcgg cggccgcgcg cgcgttcgag cagtggcagc acagctcctg cgcggaacgc
    62161 cgcgccctgc tcgaagcgat cgccgcgcgc atcgaacaga acgcgcccga gctcgccgag
    62221 atcatcgtcc gcgagcaggg caagccgctc gcgctcgcgc acatggaggt cggcggcgcg
    62281 gtcgcatgga cgcgcgccac cgccgcgctc gagctgccgg tcgaggtgat cgaggaccgc
    62341 ccgggcaagc gcatcgagct gcaccgcagg ccgctcggcg tggtgggatc gatcacgccg
    62401 tggaactggc cgctgatgat cgcggtgtgg cacatcatgc ccgcgctgcg cgccggcaac
    62461 gcggtggtga tcaagccctc ggagctgacc ccgctcaaca ccctgcgcct ggtcgagctg
    62521 atcgacgagg tggcgccgcc cgggctggtg aatgcggtgg ccggcggcgc agcgctcggg
    62581 cgcgggatct cgggccaccc cggcatccac aagatcgtgt tcaccggctc gacgcgcacc
    62641 ggccaggaca tcatgcgcaa cgccgccgac acgctgaagc ggctcaccct ggaactgggc
    62701 ggcaacgacg ccggcatcgt gctgccgggc accgacatcg gcgcgatcgc cgagggggtg
    62761 ttcggcagcg ccttcctcaa catgggacag acctgcgccg cgctcaagcg cctctatgtg
    62821 cacgagtccc agtacgagga catgtgccgg cacctggtcg ccatcgccgc gcggcagaag
    62881 ctcggcagcg gcctggacga gggcacgagc ttcgggccga tccagaaccg cgaccagttc
    62941 gagcgcgtat gcgagctcgt cgaggatgca cgcgccgccg gcgcccgcat cctgtgcggc
    63001 ggcgagccgc tgcccggcaa gggctacttc tacccgccga ccatcgtcgc cgacatcgcc
    63061 gacggcaccc ggctggtcga cgaggagcag ttcggcccgg tgctgccggt gatccgctac
    63121 cgcgacgtgg acgaggcgct gcggctcgcc aacgccagca ccaacggtct cggcggctcg
    63181 gtgtggtcgg gcgacctgga ggccgcgcgc gcgctggcga accgcctcga gtgcggcacg
    63241 gtgtggatca acggccatgc cgaggtattg ccgcactgcc cctttggcgg ctgcaagatg
    63301 tcgggcttcg gcgtcgagtt cggcctcgag gggctgctcg aatacacccg cccgcagctc
    63361 ttcaacatca acctccccgc cgcctgaatg tccgcaccgc tcggcttcct gcgccgcccg
    63421 tccgcgccgg caagcgcgag cgttgctgcg agcacggcgt cgcctccgtc cctggctgcg
    63481 ctgccggcga ccggggtggc gatcgcggtg gcgatggtgc gaccgcgcgc actggagcgc
    63541 ctgggtgcgg acacgcccgc acgcgcggca ttctggtccg ccaccctgca cgacgaggcg
    63601 ctcgcggcct ggctggcacc gctgcgcccg acgcggctgc acgtcggcag cgaattctgc
    63661 gagcgcctgc tgcccgagcc cgcgcagctc gcgcgcacgc tcgccgaggc gtccgtggcc
    63721 gggctggagg tctgcctgct cacgccggtg gcctcgcccg aggtcctgcg cgccctcgat
    63781 gcgctcttcg ccctgctgcc gccgggcgcg gaggtggtgg tcaacgactg gggggtggcg
    63841 ctgcgcctgc gcgagcgcca tccgctgctg caggccgtcg ccggccgcat cctgtgccgc
    63901 atgctcaagg acccgcgcct cggcaacccg cagtacgcac cgcccgccgc gcacggcttc
    63961 gcctcgccct ggacccggcg cctgctcgcc cgcctgggga tcgcacgcgt cgagatcgac
    64021 ctgccgctct tcgcgtccgc cgacagcttc gcgggcgtgc cgctgccgct cggggtgcac
    64081 ctgcccttcg ccttcgtcgc caagggacgc atgtgccgca ccgcaggtct cgcgcgcgag
    64141 ggcccggagc ggttcgcggc gcggcccttc tgccaccacg aatgcctcgg actcgccgcc
    64201 cgcaccgagc gcacacgcga cggtacacac gacgcctggg agaccctgca cgtgggcaac
    64261 accgtgttct gccgccactc cgccgccaac ctcgccacgc tcgccagcgc ggtcgcggcg
    64321 ggccgggtgg cgcgcctgat cgtgcccggc gagaccttgt gaggaaacgc gcatgaagat
    64381 cgtcgccccc atccggcaac tcgacgagat cgccgcgctg gcgcgcgccg gggcggacga
    64441 actctattgc ggggtgacgc cgcgcgagtg ggccgagcgc ttcggtggcg ccagcgccaa
    64501 ccgccgtccc ggcggcaacc tcccctcgct ggccgcgctc gccgaggcgg tggcgctcgc
    64561 ccaccgcaat ggcgtacagc tctccctggt gctcaacgca cagcagtatt cggtcgaaca
    64621 gatcgagttc gcgctcgcga tcgcgcaccg ctacgtcgac atggggggcg atgcggtgat
    64681 cgcgagcgac cccggcctgc tcctggcgct ggccgaagcc gagccggagc tgcgcatcca
    64741 cgtcagctcg gtggccacct gccgcaatgc cgacggcgcc cgcctctacc gcgagctcgg
    64801 cgcgcgccgg ctgatcctgc cgcgcgacat caccctcgac gaggccgcgg agatcgccgc
    64861 cgcagtgccc gacctggaga tcgaggcctt cgtgctcaac gacggctgcg tctacgagga
    64921 aggcagctgc aacaccctgc acctcccggg cgcgctcggc gggccgatct gcctggaccg
    64981 ctacgcctac gcgcaccgcc accgcgacgg ccggccgctc tcggccgcgc tcgcggcccg
    65041 cctgcaggag aacgacgagg cctatcggcg ctggctgtgg taccgcttct cctgcggctt
    65101 caccaccacc gccgacggcc tgcccttcgg cccctgcggc ctgtgcgcga tcccggcgtt
    65161 cgggcgcggc ggcatccacg cgctcaagat cgccggccgc gagggtccgc ccgagcgcaa
    65221 gctcgccagc gtgcgcatgg tccggcggat cctcgacgcc cacgacaacg gcgaagcccc
    65281 cgcggcggtg atggcccgtg cgcgcggcct gcggcctgcg cacgaacact gcgcgaccgg
    65341 cttcatgtgc tactacccgg aggtcgtctc ccgcgcatcc gaagcggcgc agccgctgcg
    65401 cgacggtcgc gcagcaggtg ctcagtagat cggctccacg agcaggcggt cgtccacata
    65461 gttctgcagc aggttgttga tcgcgaacag cgtcccggtc gaggacgacg gacaacctcc
    65521 gcaggcgccc ttgtagcgca ccgtcagggt gaattccttg agcttgacca cgtcgaggcc
    65581 gccgccgtcc tggtcgagga agggccggat gtgctcgtcg aggatctggt tgatgcgcat
    65641 cagcagttcg gcatcgtcgg cctcgacctc caccggctcc tcggccacgg cctcgcctgc
    65701 gccgatcgcg gcgcccgccg ccccacccgg tcctgccggg ttggcgatgc cgatgacgcg
    65761 gcgcgccttg tgcccctcga tcagctcggt gaccgcgggc tcgatcgccg accagggcgt
    65821 gcccgggtcg cgggtgatgg tgatgaagcc gccggtgcaa tagatgtcga cgacgccggg
    65881 taccgccagc aggctctcgc ccagggtgtc gccgacgacc gcgacaccgg cgctgaaatg
    65941 acgcggcgcg cccgtataca gcggcgcatc gagcatgaac ttgttcgcca gcgggttggg
    66001 cgtggacatg acggcggtga cattgggcat ggcggactcc tccggcttgg tagggctgtg
    66061 ctgcgactgg gggaagcgat ccgtgagcac cgggacaagg cggagtgcgg gacggattgc
    66121 gacactgcgg aacgaattct acttgtcgtt aacgtcaacg tcaattaaga tgaacttgca
    66181 tgccactcga accgcttgca ggaacctgat cccgacatga aggaatatct gctgctactg
    66241 ctcggcacgg ccctggtgaa caacgtgatc ctggtccgct tcctcggcct gtgccccttc
    66301 ctcggggtct cgggcaagct cgagaccgcg ctcggcatgg ggctcgccac caccttcgtg
    66361 ctcaccctca cctcgggcgc ggcgtgggtg gtcgagcacg ccctgctgca gccgctcgac
    66421 atgctgcacc tgcgcatcct gacctacatc ctggtgatcg ccgcggtggt gcagttcacc
    66481 gagatggcga tccagaaggc cagtccggtg ctgcaccaca ccctgggcat ctacctgccg
    66541 ctgatcacca ccaactgcgc ggtgctcggc acggcgctgc tcaacaccca gcaaggcaac
    66601 ggcctggtcg caagcatgct ctatggcgcc ggctcggcgc tcggcttcac gctggtgatc
    66661 ctcctcttcg ccggcctgcg cgagcgcatc gcgctcgcga acgtgcccgc cgccttcgcc
    66721 ggcgcgccga tcgcgctgat caccgccggc ctgctgtcgc tggccttcat gggtttcgcc
    66781 ggcctgccgg tgcgctgaaa cctccccacg aacgcgcaag gagcacgacg atgacgatga
    66841 ttctctcggt agtggcggtc gccgccctgg gcctcgccct gggctggctg ctcggtctcg
    66901 cctcgcagtg gctgggagcg accaccgacc cggtggtgga gcgcatcgcc gaggccctcc
    66961 ccggttcgca gtgcggccag tgcggcttcg ccggctgcgc acaggcggcc gccgcggtgg
    67021 ccgcgggcga cgcgccggtc acgctgtgcc ccccgggcgg gcgtgcggtc gccgagaagc
    67081 tggcgcagat cctcggcgcc accttcgacc cgggcaacct gcccgaccgc ggcccgctgc
    67141 tggcgcgcgt gcgcaccgac gcctgcatcg gctgctcgcg ctgcatcaag tcctgcccga
    67201 ccgacgccat cctgggtgcg accaagcagc tccacgtggt cctcgaagag gcctgcatcg
    67261 gctgtggcgc ctgcgccgag gtctgcccca ccggcggcat cgatctcgag ggcatcccgg
    67321 tcaccctgcg caactggcgc tggcacaagc cgggcgtggg acacgcctga acgcccgccg
    67381 caagcacacc ggaaccccac gcaccatgaa gatcttccgc ttccgcggcg gcatccatcc
    67441 cgaagggaac aaggacccca ccgcctgcca tccgatcgcc cgcctgccgc tgccgcagcg
    67501 cctgtacatc ccgctgcagc agcacatcgg cgaaccggcc gcacccgagg tcgaggtcgg
    67561 cgaccacgtc ctcaagggcc agcgcctcgc ccgccaccag ggttcggttt cggcgccggt
    67621 gcatgcgccc acgtcgggca gggtgatcgc gatcggcgac cacatggcgc cgcacccctc
    67681 cggcctgccg gtgcagacga tcacgctcga gcccgatggc gaggagcgct ggatcgagac
    67741 cgagccggcg gtcgacccct tcgcgctcac ccgtgccgag gtctccgcgc gcgtgcgcga
    67801 cgcgggcatc gtgggcctgg gcggcgcggc cttcccggcc gcggtcaagc tggacgccgg
    67861 caaccgccac ccgatcgaca cgctgatcct caacggcggc gagtgcgagc cctatctgac
    67921 ctgcgacgac cgcctgatgc gcgagggcgc cggctacgtg gtcgagggtg tgcgcctgat
    67981 cgccaacggc ctgggcgcga aacgcaccgt gatcgcgatc gaggacaaca agcccgaggc
    68041 gctcgaggcg atgcgcgcgg ccgccgcggc ccatcccggc atccacgtgc tgcgcgtgcc
    68101 ctcgcgctac ccgatgggct ccgagaagca gctcgtcacc accgtgacgg ggcgcgagat
    68161 ccccgccggc aagctctccg ccgagatcgg cgtcagcgtg cacaacgtcg gcaccgcgct
    68221 cgcggtgtgc cgcgcggtgc gcttcggcaa gccgctggtg tcgcgcgtgg tcaccgtggg
    68281 cggcggcgcg atccgcaacc cgcgcaacgt ggtggtgccg atcggcacgc cggccgggga
    68341 cctcatcgac ttctgcggcg gcttcaccga gccccccacc cgcgtgctga tgggcgggcc
    68401 gatgatgggc caggtcctgc ccggtctgga ggcgccggtg gtcaagggca ccagcggcat
    68461 catcgcgctc accgccgccg aggcgcatgc ggcgagcgaa cgtgcctgcc tgcattgcgg
    68521 gcgctgcgtg gatgtctgcc cggtcggcct ggtgccgctc gagctcgccg cgcgcgtgcg
    68581 tgccgggcgt atcgacgacg ccgccgagct cggcatgggc gactgcctgt cctgcggctg
    68641 ctgtgcctac gtgtgcccgg cccacctgcc gctggcccac ctgttcaact atgccaaggg
    68701 cgagatcgcc gcccgccgcg aagcccagcg caaggccgag gagacacgca agctggccga
    68761 ggcgcgccgg ctgcggctgg ccgccgaagc cgccgccaag gccgcgcgcc ggcccgcccg
    68821 tccggccgag acggtggaga ccgaatcatg acgagctgga ccccgaaccg ctccccggtg
    68881 gcggcgcccc acgtgcatgg gcgcagcaat attccgcagg tcatgcgcac ggtcctgctc
    68941 gccctgctgc cggcgaccgc gaccggcgtg ttcctgttcg gctggccggc gctcgccacc
    69001 gtgctgctgg cgatagccgg ctgcgtggcg gccgaggcgg cggccctggc gatcggcaag
    69061 cgcccgctgc gccacacgct cgccgacggt tcggccgtgg tgaccgggct gatcctcgcg
    69121 ctctgcctgc cgccgtgggc accgtggtgg ctgcccctgc tgggcggcgc gttcgcgatc
    69181 gtggtcggca agcaggtgtt cggcggcctc ggccagaacg tgctcaaccc ggcgatgctg
    69241 gcgcgggtcg cgctgctggt gtccttcccg ctcgagatga ccgcctgggt ggcgccgcag
    69301 ccgctgttct cgcccacggc gcccggcctg ctggacggcc tcgcgatcac cttcggcggt
    69361 ggcgcggtgc cggacgccat gagcgcggcg accgcgctcg ccccgctcaa gcacggcggc
    69421 gacttcgccg ccggcgaggc gctgctcgcg ctcgcgctcg gcacggtgcc gggcagcctg
    69481 ggcgagacct ccgcgctcgc gctgctcgcc ggcggcgccc tgctggtggc gcgccgcatc
    69541 gtgccctggc agatccccgc ggccctgctc ggcacgctcg cgctgctcgc ggcattgttc
    69601 aaccagatcg accccgcgca ctacccgccg ccgctgctcc acctgctcgg cggcagcgcg
    69661 gtcatgtgcg cgttcttcat cgccaccgat ccggtgggcg ccccggtcac cccgctcggg
    69721 cgggcgatct tcggcgcggg catcggcgcg ctgatctacg tgatccgtac ctggggcggc
    69781 tatcccgagg gggtcgcctt cgcggtcctg ctgatgaacg cggcgacgcc gctgatcgat
    69841 cgcctggtgc gtccgcgccg ctacggccgc ctgcgcacgg gcaacccgat ccccctggcc
    69901 ccgaaggagg atggccgatg agctcgatcg ctgccggcgg cctgcgccag cgcccggggc
    69961 tgctgctcgg ggcattcgcc ctcggctgca gtgccatgct gggcatgggc gacctcgcca
    70021 cccacgagga gatccgcttg cgccaggccg aagacctgcg caacgccctc gaccagaccc
    70081 tctccgccgt gccccacgac aacaatccgg ccgcggacac ggtcgcggtc gaggacgagg
    70141 gccgcgcggt cacggtgtat cgcgcgcgcc tggcgggcga ggtgcgcggt gtggccttca
    70201 actgggtgag cgaagccggc tacggcggac cgatcacgct caccgtcggc atcggccgcg
    70261 acgggcgcat cgccagcgtg cgcgtgctct cgcacagcga gacgccgggc ctgggcgaca
    70321 agatcgacgt gcgcaagtcg gactggatcc gcggcttcga cggccgcggc ctggacaccg
    70381 gcacacgctg ggcggtgcgc aaggacggcg gcgaattcga ccagttcgcc ggcgccacca
    70441 tcaccccgcg cgcggtggtg ggggccgtcc atgccagcct gaaattcttc gccggaaacc
    70501 gcgcgcgcct gctcgccgcg ccggccggcc ccggtggcga cgccgcggca actcgatcgg
    70561 agaccccgtc atgagcgaag tgcgcagggt cgtccgcgac ggcctgtggg acaacaacgt
    70621 ggtgttcgcg cagatgctgg gcatgtgccc gaccctggcc actaccggca gcgccaccaa
    70681 cgggctcggc atgggcctgg ccaccaccgt ggtgctggtg agctccggtg tgctcgtctc
    70741 gctgttccgc aacgtcatcg cgcgcgaggt gcggatcgcg atctacgtgc tgctgatgtc
    70801 caccgtggtc accctggtcg acatggcgat gaacgcctgg ctgcacgagc tgcacaaggt
    70861 gctcggcctg ttcatcccgc tgatcgtggt caactgcgcg atcatggggc gcgccgaagc
    70921 cttcgcctcc aagcgcgcgc ccgggatggc agcgctcgac ggcctcatga tggggatcgg
    70981 cttcaccctc gcgctgatgg tgctgggcgg ggtgcgcgag gcgatcggcc agggcacctt
    71041 gttcgccgat gcctcgatgc tgctcggcca gcacttcgcc tttctcgaac tgcgcctcga
    71101 gggctaccgc ggcgtgctgc tgatgatcct gcccccgggt ggcttcctcg ccctcggctt
    71161 tctggtcgcg gcacgccagc gcatgctcgc ccgcgcccgc gcgcgcagcc tcggcgccag
    71221 cgcaggtgcc gggatcaccg caggcacctg atcgaagcgc aatcgcacac tgcactgcag
    71281 gagggaagga tgaagatcag cgtcagctac gcgcaccccg agaaaccggc gtggatgcgc
    71341 ctcgatgtgg acgaaggctg cacgctgcgt gcggcgatcg agcgctcggg catcctcgcg
    71401 cgttacgagg acatcgacct ggagcgcaac aaggtgggga tcttcggcaa gatcaagccg
    71461 ctcgagaccg tgctcgccga aggcgaccgg gtggagatct accagccgat cgtgatcgac
    71521 cccaagaccg cgccgcgccg caagcgcacg ggcgcggacg gcggcgacgg gaccgagggg
    71581 gccgagcggc ctgaagggaa ctgatcgggc ggcccggatg cgggcgcgcg ccgctaggcg
    71641 ctcgggctcg tcgccgcctt cggatgcggt gcccgtgcgg cgatcgagcg cagcgcctcc
    71701 ttctcggccg cgccgatctc gtcgaggatg gcgcggatgt cttccatctg gcgttcgagc
    71761 atcgccctgc gcgcaccgag cagttccatg aagtgcgaca tctgtgcctg ctcgccgggt
    71821 gcgttgtcgt agaggtcgaa gagttcgcgc gtctcctgca gcgagaaacc cagccgcttg
    71881 ccgcgcagga tcaggctcag gcgcacgcgg tcgcggcggc tgtagatgcg ccggctgccg
    71941 cgacgggccg ggctgatcag gccctggtcc tcgtaatagc ggatcgcgcg cgtggtgatg
    72001 tcgaactcct tggcgagatc ggatatcgta tacgtcgttg cattcgccga ttcgcgcacc
    72061 cggccagccc ttccggcgat ctcttgattc atttcattct ccgttctgcg atgtccgccc
    72121 atgccccatt ctcacgccgc tcgcacgacg gggctcgcac cgggctcgca tgcaatcgat
    72181 ccgacctgga tgattgcaca gaagttccgc taacgtaaac gtcaacgaac gtccgcgcaa
    72241 ggacttgcgc agccgtgtga tccgggcgcc accgggaagt cgttgtccgc ggcgacccgg
    72301 gccttcaagg cagcgcgatc tcgaccgccg cggcggacgg atggcccgcc tgcgcggcga
    72361 agcccgcgac gccctgcagg aagatccgcg ccaccacccg ggccatgtcc tcgtaggaca
    72421 gcggaccatc cgggcgcagc caggtgaagg tccagttcat catcccgaac agcgccatcg
    72481 tgaccgcctt gtcgagcgcc acgacgcgca ggccgggctc gaccgcctcg atcgcgagcg
    72541 cgaaggcgtg caccacccgg cgttcctgtt cgtggatgcg cgcgcgctgc tcggggctga
    72601 ggaacttgac gtcctggatc agcacgacgt gctgccgccg cccgccctca tatgcctgca
    72661 tgaagcgcag gatcaggcgc tcgagacgct cgcgcggcga ctctgccgcg gcctcgacat
    72721 ccgccacgat cgccagcaga tcctcgacgt ggcccgcggc gatgtcgtag aggatgtgtt
    72781 ccttgtcgcg gtagtagtga tagagcagcg ccttggaaac cccgcacgcg cgcgcgatct
    72841 ccgccatcga ggcgttatgg aagccttcgg ccgcgaagag gcccgcggcg gcgtcgagga
    72901 tcgtcgcgcg ctgcaactgg aaggtcggcg ccttgcctct ggccatcggg gtctcgctcc
    72961 tgtgctgcgg ccgctccgtg gtgcgcaggc gcacgcacgc cgaccgggtt gtccggggca
    73021 caccgagggg gcacgccgca cgccctcggc gcctgtcgtc gccgatcgag aggatcgtcc
    73081 gcatcgcctc gcccctgcat ccttgccggt cgcgggctgg tgcttgcgct cgatcgccgt
    73141 taacaggatt gtgcccggca acagatccga ccaaccagtc gatcaataac aatgttgcgc
    73201 tacatcaaga cacgagtggt tttcttgcac cccggcttgc gtgccgcgcc cttcccatgc
    73261 actcacgcgc gccctgcacc gcagccggga ccggggcccg tcctcgatcc gcaggccgcg
    73321 catcgacgcg aggggcgacg cgccccgcgc accggcatgc tactgtcggc cgcacgagtc
    73381 ccatcccatc ggagcccagc catgccctgc tacgaaatcg acggcgtccg cccggtcgtc
    73441 catcccaccg cctacgtgca tcccgacgcc gtgctgatcg gcgacgtgtt cgtcggcgcc
    73501 ggctgctacg tcgccccgct cgcctcgctg cgcggcgact tcggccgcat cgtgctcgag
    73561 gagggcagca atgtgcagga cgcttgcgtg ctgcacagct ttcccggcat cgacaccgtg
    73621 gtcgaggtca acggccatgt cggccatgcc gcggtgctgc acgcctgtcg gatcgggcgc
    73681 aacgcgctgg tcggcatgaa cgcggtggtg atggaccatg cggaggtggg cgaaggcagc
    73741 atcatcgccg ccgccgcctt cgtgaaggcg gaaatgaaga tcccgcccgg cgtgctggtg
    73801 gcgggtgtgc cggccaaggt gctgcgcgag ctcaccgagc aggagcgcaa gtggaaggtg
    73861 atcggcaccg actgctacca gaacctcgcc cgccgcagcc tcgccagcct gaagccctgc
    73921 gaaccgctgc gcgagggcga ccgcgagcgc gcgcgcttcg agatgcccga cgtcatgccg
    73981 ctgagcgacg cgcgccggcg tgccggctga acgctcgacg cggctgcgcc ggaccgctca
    74041 cgcgaaggga tagggccact cgtcctgtcc gtcggagaac agccccgcga ccagcgtgcg
    74101 cagccagcgg ttgcgcgggt cgtggtggaa gccccggtgc cagtgctggt acagctcgat
    74161 atgcgggatc gccagcggcg gctcggccat gcgcaccttc tcgcccacgc gcgtgaagta
    74221 atgaccgatc gcatgcggca gcgtcaccac caggtccgag cgcccgatga tgccgggcag
    74281 gctcatgaag tgcggggtgc gcagcacgat gcggcgctgg atgcgcagac gctcgaggta
    74341 tttctcgaag atctcctggc tgcgcccctt ggcacggacc gcggcgtgct cgagcgagag
    74401 gaagcgctcg agcgacagct gacggtcctg caccggatga tcggcacgca acaggcacac
    74461 gaagcggtgg tcgagcagct tctcgcggta gaagccggcg gcgtcgaggt cggggtaata
    74521 gccgatcgcc agatcgacct ctccatcctc catcgcgcgc atcagctctc ccggcgcgag
    74581 cgtcaccgaa tcgagcatgg ccagcggcgc gaggcggcgc acgctgtcga ggatgcgcgg
    74641 caggaagacc agctcgccca cgtccgagag ggcgacggtg aggttgccct cgtatttcgc
    74701 cgggtcgaag cggtcggcga cgaagaaatc ggtttccagc cgcgccagca tcgcccgcgc
    74761 gctgctcgcc agcgcatgcg tgcgcgcggt gggcgtgaca cccgacgccg tcttgatgaa
    74821 cagcggctcg ccgagcgcgc ggcgcaggcg gccgagcgca ctgctcacgg tcggctggct
    74881 caggccgagg tgctccgcgg cacgactcac gctgccggcc tcatagaccg cgagcaggac
    74941 acgcagcagg ttcaggtcga agtcgcgcag actcatgctg gcccgagtgt tatcgatagg
    75001 gtcaataaag gtaatccgtt cattggtatt gtgcagcatc gcccggggga atttaatggt
    75061 ccggacaaac agatgacgtc cccgcccgcg aggggagtcc tgccgaggag acacaccgat
    75121 catgtccacc catcccattt cgggccacgc gcccgcgctg cgaaccaccg tctgcggagc
    75181 gacatcatga acgccgccga atggctgctc gccggcgatc ccgcgcggat cgcgctgatc
    75241 gagggcgatg tccgcatcga ctacgccggc ctgcgtgccg attccatccg cagcgccgcg
    75301 gccctgctcg acgccgggct gcgccccggc gacgcctgcg tgctcgcgct gcccgacggc
    75361 atcgaatggg ccgccgcctt catcggcatg ctgtgggcgg gggtgcggcc gatcgcgatc
    75421 aacccgcgca ccgccaccac gcaactcgcc gacctgatgc tggactcggg cgccgccgcg
    75481 gcgctgctcg aggacgaagc cgcgcgtgcg ctcggcgaca agcgcgcgat cgacctcggc
    75541 gaatggcgcc gccgggtgga gcgcgccacc gcgacgcccg cagccgcgca ggccgccgac
    75601 gacgatccgg cgttcctgct gtactcctcc ggcaccaccg gccgccccaa gggcatcctc
    75661 catgcccacc gcgcgatccg tgatgcccac gtgttcgccc gcgaccttct cggtgcgcgc
    75721 ccggagcacc gcttctattc cagctccaag ctgttcttcg cctacccgct cgccaacgcc
    75781 ttcttcgccg gcctgcgcct gggcgccacc gtcgtgctcg accccgaatg gcccgacccg
    75841 gcgcgcgtcg cggcgatggt cgagcgccac gagccgcaca tcttcttcag cgtgcccacc
    75901 ctctaccgcc gcctgatcga tgccggcgtg cgtttccgcg gcgtgcacgc cgcggtctcc
    75961 gccggtgagg cctgtccgcc cgcgcttgcg cgcgactggc aggcgatgac cggagtgccg
    76021 ctggtcaatg gctacggcac caccgagacg ctgtcgctgg tgctctaccg cacgccggag
    76081 atggacgctg cgtgccccac tccactcacc gagatccacc cggagcagct caccagcggc
    76141 gagctcgaga cctggcggct gtggttctcc caccccgcag tcgcgctcgg ttatacgcgc
    76201 gtggtcaccc acgacagcgc gcgcttcgcc gacggccgct tcgcccccgg cgacgtgttc
    76261 cgccgtgccc ccgacgggga aggctggctg ttcgccggcc gcagcgacca gctcgtcaag
    76321 gtgttcggcc gctgggtgga cgtggtcgcg gtcgagcagg ccgtgcagga acgcatgcgc
    76381 ggcaaggccg aggaggtgtg cgtgatcccg gcgcagggcg aggacgcgga catgatccgc
    76441 ctgcacctct tcgccattcc cggcgacctg ccgccaccgc aggtgctggc cgccgcgcag
    76501 gccgcgatcg agagcctgcc gccctaccag cgccccgaga agatccacct cgtggaccac
    76561 ttcccgcgca ccgacaccgg caagctgcgc cgcaacgagc tcgcgcgcag caccggctga
    76621 agcgcgtcct tgtcgagcgc ggccccggac ctgtcaggca ccggggccgc tcgccgcccg
    76681 gtcgagcgcc cgctcgacgc tgtcgatctt gtcctgcatc gtctgctcgc ggtcggtgcg
    76741 cgtgcccagg cggatcgagg tggtgatgcg cggcgcgccc atcgcatgga cgacctcgtg
    76801 gcagcgcttg accgcggcca tcacctcgtc ccactcgccc tcgatattgg taccgtaggc
    76861 atggagctgc gacacgaggc ccgcctcgcg gatcacccgc tcgcaagccg ccacatagga
    76921 tgaaaccgac agccccacgc cgagcggaac cacgcacaga tcgagaatga ctttcacgga
    76981 cgcctccacc agcgctcttc gaaacgcccg attctagcgc cgcaaccggc ccctgcgcgc
    77041 cgccgccgga gcaccagcgg gcaggacctc cgcaatttcc gcgcccgcca gctcgcgcca
    77101 ctcgcccacg cccaatccat ccagcgtcca gggcccgatc gccacccgga tcagccgcaa
    77161 cgtgggatgg ccgaccttgg ccgtcatccg gcgcacctgc cggttcttgc cctcgcgcag
    77221 cacgatctcg atccagctcg tcggcaccga cttgcgaaag cgcacggggg gatcgcgcgg
    77281 ccacagccag tcgggcgccg gcatgcggcg cgcggcgcac ggcaagctgc ggaagtcccc
    77341 cagatccacc cccgcacgca ggatcgccag cgcggcctcg tcgatctccc cctccacctg
    77401 caccagatag gtcttggcca gcttgtggcg cggatcggca atgcggtgct gcagggcacc
    77461 gtcgccggtg agcagcacca ggccctcgct gtcggcatcg aggcggcccg cggcatagac
    77521 accgggcacc tccacgtagt ccttcagcgt gcggcgaccg gcttcgtcgg tgaactggca
    77581 cagcacgccg aacggcttgt tgagcagcaa gaggcgtgtc tcagctcgtc cttcggcagg
    77641 agtgtcggat tttcttacac ccgatgcagg ctcgcgcttg ggcactcgtt tcggcaaatc
    77701 catgaaattc ctgtgcttcc tcattccagg ggcttcttag gcatgtcggc aagaattcca
    77761 taacgatgaa tttggccggg agcatgcgcg atgccgtttc gtcgatgtca gcaaggggct
    77821 gcccgccggc aagcaccgcc gcgcccacga agtgctggtc ctgatcggca agctctacgc
    77881 catcgagaag aacacgcgca aactcaaggc caccgagtgg ctcgcgctac gccaaggccg
    77941 aagccgcgct gtcatcaatg aactgcgctg ctgtcgtgac caagtgctcc ctaccgtgcc
    78001 accgtcctcg gtgctcggag gtgcgctggg cgacttgcat cggcagtggc cgcgtccgac
    78061 gcgctacctc gagcgcggcg atctgccgat cgacaacaac ccggccgaaa acgccaaggc
    78121 caacggcgtc gagccgtacc tgtggctgcg ccaagtgctg cgcgccttgc ccaccacgac
    78181 caccgtcgaa cacttcgagg ccctcctgcc ttggaatctc gaggcggagc agttgatcac
    78241 atacggagtg agaattctcc acaaacaata aagccccggc tagcggggct ttttgtcgcc
    78301 atcttcaagc tgctgcgcgc tcaatggaat
//
LOCUS       DAOQBS010000002        62934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_178_length_62934_cov_15.192974, whole genome shotgun sequence.
ACCESSION   DAOQBS010000002 DAOQBS010000000
VERSION     DAOQBS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 62934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62934
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178_length_62934_cov_15.192974"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /gene="bamA"
                     /locus_tag="PKN25_00345"
     CDS             complement(<1..1590)
                     /gene="bamA"
                     /locus_tag="PKN25_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNV89372.1"
                     /translation="MNRKLLPGLLVALFAAAPALAFDPFVVKDIRVEGIQRTEAGTVF
                     NYLPVRVGETFTEAQAADAIRALFATGFFSDVRIETEGNVIVVVVDERAAIADITFDG
                     VKEFDKDALKKGLREVGLAESRIFDRSLLERAEQELKRQYLSRGKYAATVTTTVTPLE
                     RNRVGINFKVDEGGVAKIRQIRIVGARAFEEEDLLDEMQLTTPGWLTWYTKNDQYSRQ
                     KLSADLETLRSWYLNQGYLDFNIDSTQVSITPDKQDIYITVNITEGEKYTVTGVRFTG
                     DLILPEAEYQALTKIRPGETFSRERLTETTKAITDRLGNEGYAFANVNAAPEVDKVKR
                     EVAFTVFVDPGRRVYVRRINVGGNTKTRDEVVRREMRQMEGGWYDAAAINRSRTRIDR
                     LGFFDEVTVETPAIPGTTDQVDVNFTVKERPTGNLMLGAGFSSSENIVLSASISQQNL
                     FGSGNALTLGLNTSQSSRTLALSFTNPYYTVDGISLGWDLYHRTYDADELDSVSPYKT
                     VSTGAGIRLGWPIAEDDHVNFG"
     gene            complement(1599..2963)
                     /gene="rseP"
                     /locus_tag="PKN25_00350"
     CDS             complement(1599..2963)
                     /gene="rseP"
                     /locus_tag="PKN25_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNV89373.1"
                     /translation="MTLFDYLVPFALALGLLILVHELGHYLVARWCGVKVLRFSIGFG
                     KPLLRHTAGSDGTEWVLAAFPLGGYVKMLDEREAPVAAPELHRAFNRQSVYRRFAIVA
                     AGPLANFLLAIALYWGLFVGGTEELKPRVALSDTPAIAQAAGVREGDLVIAVDEEPVR
                     SWQDLRWVLLRHALDNREVVLRVRTLEDVDAFRRMDLSGVAIDEGNTDLIARLGLRPW
                     RPALPAVIGRIADGSAAERAGLQVGDRVLAISGTAVAAWADLVRLVREAPGRALDFEI
                     DRAGGVVGLVVTPDAAEEGGARIGRIGVGVGEAATGGIEMFGEIRYGVLEGLARAVRQ
                     TWETSVLSLKMIGRMLTGEVSWKNLSGPVTIADYAGQTAQLGLAHYLKFVALISISLG
                     VLNLLPIPVLDGGHLLYYTVEIIKGGPIPERIMEVGQQIGLALLVMLMAFAFYNDLNR
                     LISG"
     gene            complement(2960..4162)
                     /gene="ispC"
                     /locus_tag="PKN25_00355"
     CDS             complement(2960..4162)
                     /gene="ispC"
                     /locus_tag="PKN25_00355"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNV89374.1"
                     /translation="MPDPAPQRLTVLGATGSIGQSTLDVVARHPDRFEVFALSAQRQD
                     DKLLEQCLRFSPRFAVMGDATAAERLRAGLRAAGRDTEVLCGEEALERVAAANEVAMV
                     MAAIVGAAGLRPTLAAARAGKKVLLANKEALVLSGRLLIDAVAASGAVLLPIDSEHNA
                     IFQALPAGYAREPARAGVRRVLLTASGGPFRTAPIDSLANVTPEQACAHPNWVMGRKI
                     SVDSATMMNKGLEVIEAHWLFGAPADMIEVVVHPQSVIHSMVDYADGSVIAQLGNPDM
                     RTPIANAMAWPERIDAGVRPLDLFEIARLDFERPDFARFPCLKLAFDALRAGGTAPAV
                     LNAANEEAVAAFLDHRIGFLDIAAVIAASLERSRPGAVDSLEAVLAADAHARELARDE
                     ISRLRRDR"
     gene            complement(4208..5029)
                     /locus_tag="PKN25_00360"
     CDS             complement(4208..5029)
                     /locus_tag="PKN25_00360"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNV89375.1"
                     /translation="MLKDRVLTAIALLVGLLAAVFLLPASVWLVLCAVLCGAAAWEWG
                     ALARFERGLRVSTAVAFAAASAFLGWSAGLGSGELGGAAAVVPLYVFSALFWIFVVPM
                     WLRGKWALSNPMLASLVGFVVLVPTALAMMQLRTMGPGVLLGTMAVVWVADIAAYFSG
                     RAFGRHKLAPAISPGKTWEGAAGGVLAVLAFGFGVLAMRGAEFEGGAPVIAGVLLAWT
                     AVSIVGDLFESLLKRQAGLKDSGNILPGHGGILDRIDSLTSTLPLVALGAVWLAR"
     gene            complement(5037..5804)
                     /gene="uppS"
                     /locus_tag="PKN25_00365"
     CDS             complement(5037..5804)
                     /gene="uppS"
                     /locus_tag="PKN25_00365"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNV89376.1"
                     /translation="MIGGLFRSSTRDIPDVSQVPKHIAVIMDGNGRWARKRMLPRVAG
                     HARGVEALRATVRGCVDRGVEYLTVFAFSSENWRRPQDEVSFLMQLFLKSLQKEIRRM
                     HDNDMRFRVIGDLSKFDPRLVDMIREAETLTAGNGGLNFTVAANYGGRWDILDAMSRL
                     MAREPGRLERFTEDEIASELSMSFAPEPDLFIRTGGEKRISNFLLWQLAYTELYFTDV
                     LWPDFDDGALGQAIGSYQGRERRFGRTSEQLKADAGE"
     gene            complement(5857..6414)
                     /gene="frr"
                     /locus_tag="PKN25_00370"
     CDS             complement(5857..6414)
                     /gene="frr"
                     /locus_tag="PKN25_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNV89377.1"
                     /translation="MISELKKNIEQKMLKSVEALKTDLAKVRTGRAHTGLLDHVMVEY
                     YGSMVPVNQVANITLIDARTIGVQTWEKPMLGKVERAIRDSDLGLNPANMGELLRVPM
                     PALTEERRRDLTKVVRHEGETAKVAIRNLRRDGNQQLKDAVKDKAISEDDERRGEDEI
                     QKLTDRYVAEIDKLLAQKEQELMQI"
     gene            complement(6433..7146)
                     /gene="pyrH"
                     /locus_tag="PKN25_00375"
     CDS             complement(6433..7146)
                     /gene="pyrH"
                     /locus_tag="PKN25_00375"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNV89378.1"
                     /translation="MSAAYKRILLKLSGEALMGDDAYGINEEVVSRIVSEVAEISRLG
                     VQVGVVIGGGNIFRGMKGAASGMDRATADYMGMLATVMNAMALADAMRRMGLEARVQS
                     ALRIDQVVEPYIRGRAIRHLEEGRVVIFAAGTGNPFFTTDTAAALRGAEIGAQIVLKA
                     TKVDGVYTADPKKDPQAQRYHRISFDEAIGRNLQVLDSTAFALCRDQKLPINVFSIFK
                     PGALKRVVMGEDEGTLVHS"
     gene            complement(7157..8053)
                     /gene="tsf"
                     /locus_tag="PKN25_00380"
     CDS             complement(7157..8053)
                     /gene="tsf"
                     /locus_tag="PKN25_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNV89379.1"
                     /translation="MAEITASMVKELREKTDAPMMECKKALAEAAGDMGKAEEILRVK
                     LGNKASKAAARIAAEGVVGIDISADGKLGSIIEVNCETDFVAKNDDFLALVQNCAGLV
                     AAKSPADVAALSALPLGEGTVESTRTALVGKIGENMSIRRFARIEAKGKLFSYIHGGA
                     KIGVLLDVVGGDEQLGKDIAMHIAASKPKALDASGVSQDLIDAERRIAIEKARADNKP
                     EAMLEKIADGTVQKFLKEVTLLAQVFVKAEDGKQTIEQLLKAKGASVAGFTLFIVGEG
                     IEKKVTDFAAEVAEQAAAAAKK"
     gene            complement(8219..8974)
                     /gene="rpsB"
                     /locus_tag="PKN25_00385"
     CDS             complement(8219..8974)
                     /gene="rpsB"
                     /locus_tag="PKN25_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNV89380.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRFWNPRMAPYIFGQRNKIHIVNLEK
                     TMAKYNEAMEFVRKLAANRGNIMFVSTKRQAREIIAEEAQRAGMPYVDERWLGGMLTN
                     FKTVKQSIKRLKEVEAMMEDGSIERLSKREALTVRRELEKLQKSIGGIKDMGGLPDAL
                     FVIDVGYHKIAITEAQKLGIPVVAVVDTNHSPEGVDYLIPGNDDSSRAIRLYARGVAD
                     AVLQGRSQVLQEIVDAGGDEFVEVEEEGSQEQA"
     gene            9370..10200
                     /gene="map"
                     /locus_tag="PKN25_00390"
     CDS             9370..10200
                     /gene="map"
                     /locus_tag="PKN25_00390"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNV89381.1"
                     /translation="MSIVIKTPEEIEKMRVACRLAAEVLDYIEPFVVPGVTTGELDRL
                     CHDYMVNVQGTIPAPLDYAPPGYKPYPKSICTSVNHQVCHGVPGEKALKKGDIVNLDI
                     TVIKEGFHGDTSRMFLLGGDSACSIVARRLTRITYECMWLGIATVKPGGRLGDIGAAI
                     QKHAEGNGFSVVREFCGHGIGRKFHEEPQVLHYGKAGTGPTLEPGMIFTIEPMINAGK
                     AAISELPDGWTIVTKDRSLSAQWEHTVVVTETGVEVLTLSAGCPPPPELVAARFATAG
                     "
     gene            10223..12799
                     /locus_tag="PKN25_00395"
     CDS             10223..12799
                     /locus_tag="PKN25_00395"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNV89382.1"
                     /translation="MNDALTDELHTLATTARQRLVEGTAQLRDAYQGRPQTSILLKGR
                     ARLVDHELVRLWTACAMPASAALVAVGGYGRGELFPASDVDLLVLLSTPPDEALKARL
                     SALVGALWDCGLEVGHAVRTVDECMIAAADDITVQTNLLEARLLAGDPALFAELSARY
                     REALDVRAFYKAKLLEQEQRYARFNDTPYALEPNCKESPGGLRDLQMLGWIARAAGLG
                     HSWRDLARHRLITGSEAGELRAIERFLQHIRIRLHHLTGRAEDRLLFDHQEKLARILG
                     IEATPTRRASEVLMQRYYVTAKMVMQLNTVLLQNYGSVIFPDRGAAIVVNARFQVVRE
                     LLDIRDEDVFERHPSALLECFLLLQQRSELKGMTARTLRALWIHRNQVNAAFRADPAH
                     RVLFLQILQQRRGIVRVFRKMNEYGILSRYIPAWRRIVGQMQHDLFHVYTVDQHILMV
                     LRNVRRFTMGEHAHEYPLMTRLIMAFERHWLLYVAALFHDIAKGRGGDHSTLGMADAR
                     EFCTQHGMDANDTALVVWLVEQHLTMSRVAQKEDTSDPEVIQRFADVVRDERRLVALY
                     LLTHADIRGTSPKVWNGWKGKLLEDLFFATQRLLRGATAKEALGLDGRLEDARRQLRF
                     HGLRPGVEDALWARLDPVYFMRHSAEEIAWHTRLLYYRTESDEPVVKARVAEDEDGVQ
                     VMVFTRDQKDLFVRLTGFFGRMGFTIVDAKVHTTRHGYALDSFMLQDPGEQAAYRDIV
                     ILIEHELTARLQKPGLPERPSRGRMSRQVKHFPISPEVSIRPDEAGRHHVLSLTAADR
                     PGLLYDVAAVLAEHGIRLHTAKIATLGERVEDTFLLTGNVLNQDAQVLRIERELLERL
                     QV"
     gene            complement(12839..13345)
                     /locus_tag="PKN25_00400"
     CDS             complement(12839..13345)
                     /locus_tag="PKN25_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNV89383.1"
                     /translation="MKRIESGFEHVLFTSRWLMAPVYFGLVLAMVLLLVKFSKELFGL
                     FAQIWSASGSELIIGVLSLVDVALIMNLLIIIMFSGYENFVSKMEDLHTHRDRPEWMG
                     HIGFSDLKLKLIGSIVAISGIELLKAFMNAHNLTDRQLGWMVGIHVTFLFSGLLYAAM
                     DRLAGKGH"
     gene            complement(13371..14108)
                     /locus_tag="PKN25_00405"
     CDS             complement(13371..14108)
                     /locus_tag="PKN25_00405"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HNV89384.1"
                     /translation="MLAIIGGSGLTQLSNMEIERREVVRTPYGEPSGALAFGRVCDEP
                     VVFLARHGYGHTIPPHLVNYRANIWALKQAKATGIVSVASVGGIRADLAPGTLIVPNQ
                     IIDYTWGRKSTFFEGGDTPVRHIDFTEPYDETLRHRLLAAIAAAGDSAMDGGVYASTQ
                     GPRLETAAEIDRLERDGCDIVGMTGMPEAALARELELPYAAIGVVVNYAAGRSSSAHG
                     IHFDDIELVLQESMVRVRAVLEGLCHV"
     gene            14230..16047
                     /gene="typA"
                     /locus_tag="PKN25_00410"
     CDS             14230..16047
                     /gene="typA"
                     /locus_tag="PKN25_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNV89385.1"
                     /translation="MSRAIRNIAIIAHVDHGKTTLVDQLLRQSGTFRENQQVAERVMD
                     SGDIEKERGITILSKNCAIQYGDTHINIVDTPGHADFGGEVERVLSMVDSVLLLVDAV
                     EGPMPQTRFVTRKALALGLKPIVVINKIDRPGARPDWVINHTFDLFDKLGATEEQLDF
                     PVIYASGLNGFAVINEGDERKDMRPLFEAILEHVPAPEVDANGPLQIQVCSLDYSTYM
                     GQLGIGRIKRGSVRPNQEVVVMYGEENRGKGKVSQILTFEGLERKPSELAEAGDIVLI
                     AGIQQVNIGVTLCDPECLEGMKPIAVDEPTLTMNFMVNTSPLAGREGKYVTSRQIRER
                     LEKELLKNVALRVNYTGDSDVFEVSGRGELHLTILLENMRREGYELAVGRPRVVYKEI
                     DGVKCEPYEMLTVDVEEDHQGSVMEELGRRRGELQDMQPDGRGRVRLEYRIPARGLIG
                     FQGEFLTMTRGTGLASHVFDDYGPMAGAMAERRNGVLISQNDGDAVAYALWNLQDRGR
                     MFVSPGEALYEGMIIGIHTRDNDLVVNPIKGKQLTNVRASGTDEAVRLITPIQLTLES
                     AIEFIADDELVEITPKNIRLRKRHLKETDRKRAAKADAS"
     gene            16533..18923
                     /locus_tag="PKN25_00415"
     CDS             16533..18923
                     /locus_tag="PKN25_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNV89386.1"
                     /translation="MNESECAGRLIWFKATAGNERFHTYVFQQRVGVLIDWFRVLRSD
                     ERGDRFRAWGIINDPACCVPGSPDCPAKSLDETYGLDWCPGDEELLPHVGRSGYRDPA
                     CDFRDAALKPGDVHGPADQRHDACDLRFGTSTGALGIRKFPNPRFDRAAWLKLNGSPG
                     TWEGYNRPIAAAGGRGEAARSHLQDGAIEPPFLIGTSCGSCHIAFDPLNPPADPARPA
                     WENIKGLIGNQYTRVSEIMVSGMASNTLEWQMFAHARPGTSDTSAIPTDQVNNPGTIN
                     ALINLAKRPTFANESVIKWRKTASCAKGVDETRCWCEPGRNGKCWEKSLHKEAVHHIL
                     KGGEDSIGALEAIQRVYFNIGSCSEQCWVNHLADLRQLDPQQRGFGQTPFDIGQCRRD
                     CPNFRAVEDRLGNILDFFMSAEGHATDLREARENTLRVANPGARYTDQDLIADLERTF
                     GAGAVARGREVFANGCARCHSSQPAAATGQLAGLDFHKLDLKTGLREDWLGNDGPTAV
                     SEVGTFRCRALHSNHMAGHVWQEYGSETLRAQPPDPNVKEPGDGGRGHYRNISLLNLW
                     AHAPFLHNNALGPELCGKPHNAANDFYAQRPRYVDASNVKLLPAERQPACFEYDPSVE
                     GRFKLYTLSMQALLNPAQRIPKVTLLNEDVTLRLGPRLWDGTDKERLLGFELTIPSEI
                     DGRGVTAGTLGNFQHKAFIVDLVRAKTAPDELERSLAKRLGKESARKVLADLQAVARE
                     VTGKPNGLVEALRARPYLIKQVYSSCTAEVENAGHRFGEDLSEADKNALIAFLATL"
     gene            18941..19864
                     /locus_tag="PKN25_00420"
     CDS             18941..19864
                     /locus_tag="PKN25_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89387.1"
                     /translation="MTRISHWPFGALAVCMLAAMAVTGCGLFDDDGERAPEIAFAPHA
                     EGYAGKPDFAWLEHRHPLSTADLYKITPQNIAQLDQEQVDQIYARLSAGPIPDGAFDG
                     DLFFPRGGSGKLRLSEIAGGGLKGLLVNVAGGKLDLVGEMLWKGKVFYRDERLLRNRI
                     EDLTTLRKLGLVEESADTPLQKLNVGGKDQWLLFPAKLYCGQSLLDGRRESIIIDYAF
                     TDELPGYRRMPDLLAGRNGFAIRDEIRMVYPGFYLGRAYMDRAFVVNFVLHNAQMIAA
                     SREAFLKTGQVDQDCWSGTQQRLIAAAAGGG"
     gene            19936..22077
                     /locus_tag="PKN25_00425"
     CDS             19936..22077
                     /locus_tag="PKN25_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89388.1"
                     /translation="MRLSRPVSRHVLPVLLGALFGSVFMAPANAAEVDRAPSPHGRSL
                     FDRLLERGANGAQQVPFPFPALLQRVRAQLDGEDPNGGLAIVLIPLGRSLQRHAAGDV
                     DAFRFPRVVAAVTGEPASDAPAGHLYLKDRLYVAHHERAEALEVISYNEDAGRFEFQL
                     VLDYRAGARPRVVAANRTLCLACHQNEAPIFSRPSWDETSASPPVAARLAATGLDYQG
                     LPWRHGVDVPEAIDAATARANRLGVAQRLWRDGCALATPQAGIDCRAQALLHALRQRL
                     IGTGAPVARADATNRLVDPLLANWQARWPDGLVIPDPQIPNRQPFAGIEGGIAPPSDA
                     ELVRYADIPAVFDPLALRAPIEHWRGRDPGDVMRFIHLLGTLFSRADLARIERALARM
                     PQPAAREIDLSCTLRRATDATRLDLDCARGSEVRLQARIRLDGGRSADGILDRLELSG
                     SSAHTGVTLAIAKDHDPASGWPIRFALTAKAGGPIRASSGEAITTLRLGGDDGSAHLP
                     GSPSAAEAVVAALELVDDLEPLGHAVEALAQANVAGQDDALTGGPARRMALLAPLLRG
                     LGEDVPAPPAAAAPPPALMADVTRSRTTHWPADLQPFLRQCSQCHAENTAFPPGFLHG
                     DEAQVRANLATCAQRMLHRLDMNRLPPAARSKTPMPPPAAAHAGAFLQSPDLAAMRDL
                     LEHVLQASGVRSADVLAHPYATLAPCRVPTG"
     gene            22126..23673
                     /locus_tag="PKN25_00430"
     CDS             22126..23673
                     /locus_tag="PKN25_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNV89389.1"
                     /translation="MNTPESVPTTHRLRLARTLLRILVLLPWLLLLLAGGYALVRFVP
                     DEPVVHADPVEHFKYGSTGGERASGFPYWIWQALPTVCAEHLPGPGYASLGLIFEEGR
                     DLPVGVSKRRHLGIDRVFLNCAACHAGLVRDTPAAPARLVVGMPAHRFDILAFQRFFF
                     ECAAGPTFSREFIVPEIERLSGGLGLVDRYLVYPVAIALMRERLLMLRARFGFVSAQA
                     PWGPGRVDTFNAAKVLFNFPVQRLPARERLGAADFPSIWNQRKRMQRDDGERMELHWD
                     GNNTHTEERNKSAAFGTGTTPPTIDLAAISRVEAWLLDLGPPAWPLPVDKTLAARGAA
                     LYARYCADCHGASGSDFAGPKVGHVTPIADIATDPARLDSYTRDLAVNQATLYAGYPH
                     RFRHFRKTWGYANMPLDGIWLRAPYLHNGSVPTLRDLLEPAFARPTCFVRGGTLIDAV
                     RVGFASAADPAQCAAPTGRAQTATDHFLFDTTLPGNGNSGHEGPAYGTDLPAADKDAL
                     VEYLKTF"
     gene            23690..25201
                     /locus_tag="PKN25_00435"
     CDS             23690..25201
                     /locus_tag="PKN25_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV89390.1"
                     /translation="MKFAPCTNPGPRPTRLLGRRRNTGRSLLVALLVLLALGAAGGAF
                     AWYKFFREEAQPEWITSDPDMRFKYGSIGAERDAGIPYWIFYVLPRMFPDKLPGPGGY
                     AAFGVVWEEGQELPVGFSKKVVGFPRVANNCASCHTTSYRTQADAAPTFVPTGPNHTL
                     NLWAFFRFLVDCAKDPRFNADNLMAEINLVTDLSFIDRLLYRFVIIPITRKRLLEREQ
                     QFAWLYRDDFPPWGRGRDDAMNLTKYFMIRWPMDDSFGPTDMPSLWNLGKYRADQGMR
                     MNFAGDSHDAYSVVIDSALGLLGAPPKDNAEFLGEVRWLIEYVSAKRAPPYPFAVDTA
                     AVARGKQVFDTTCAGCHASARTGTVIPLAEVGTSAERIATWNERAAREANQVVAGMGI
                     ERPGLVEAPLTGYVAAFLDGIWLRAPYLHNGSVPSLRDLLEPPAQRPTRFWRGYDVYD
                     PDRVGFVTHGPEAERIGTVHDVGARGGSNRGHAFGTTLPATDKADLLEYLKTM"
     gene            complement(25258..26163)
                     /gene="galU"
                     /locus_tag="PKN25_00440"
     CDS             complement(25258..26163)
                     /gene="galU"
                     /locus_tag="PKN25_00440"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNV89391.1"
                     /translation="MKKVTKAIFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVAAGITDMIFITGRTKRSIEDHFDKAYELETELEARGKKQLLEIVRQTVPKGVNCIY
                     IRQSEALGLGHAVRCASHVVSDEAFAVILADDLLDNKDGTPVLKQMVDVYNYHRCSVL
                     GTMNVPREETRSYGIVSTESDKGQVQRMTGIVEKPKPEEAPTTQAVVGRYILTPRIFD
                     HIRALKPGAGGEYQLTDAIASLLKEEAVLAFQFDGVRYDCGSKLGYLKATIEFGLRHP
                     ETGGDFAAYLSGRFGAEGKAAKAAK"
     gene            complement(26243..28324)
                     /gene="ligA"
                     /locus_tag="PKN25_00445"
     CDS             complement(26243..28324)
                     /gene="ligA"
                     /locus_tag="PKN25_00445"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNV89392.1"
                     /translation="MDLFAEDGRGETAADAAARAAALRAEIARHDHAYYVLDAPTIPD
                     AEYDRLFRELQAIERAYPALRSADSPTQRVGGKALAQFATVRHRVPMLSISTETDTEA
                     SGAFAFDARVRRALGLDEDDAAVAYAAELKFDGLAISLRYEDGVLVQAATRGDGETGE
                     DVTSNVRTVKAIPLRLLGEAPPVLEVRGEIYLRRDDFERLNARQAEAGEKIFVNPRNA
                     AAGSIRQLDPGIAARRPLSFYAYGLGEVAGWTLPATHTEVLDALAAFGLPVCGHRAQV
                     RGAEGLAAFHARIGALRDTLPFDIDGVVYKVDALALQQQLGFVTREPRWAVAHKYPAQ
                     EAVTLLRDIEVQVGRTGALTPVARLEPVFVGGVTVTNATLHNQDEIDRKDVRIGDWVI
                     VRRAGDVIPEVVAPILERRGGELPRFVLLDRFPTCPVCGSHVVRGEDEAVARCTGGLF
                     CPAQRKQALLHFAGRRAMDIEGLGDKLVDQLVDAAIVKTPVDLYRLGVLALANLERMG
                     EKSAQNLLMAIEKSRGTTLARFIFALGIRNVGEATARDLARHFGKLDALIAADVDALQ
                     QVPDVGPIVAKCIAEFFAEPHNREVIEQLRAAGVHWAEGEPQGSVAGALAGKTFVLTG
                     TLPTLSRDEAKARIEARGGKVAGSVSKKTHYVVAGAEAGSKLDKAQALGVAILDEDGL
                     RALLNETDTNA"
     gene            complement(28324..29406)
                     /locus_tag="PKN25_00450"
     CDS             complement(28324..29406)
                     /locus_tag="PKN25_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HNV89393.1"
                     /translation="MDSELQIALIGAGIAAVVLVVGYNKWQERRHRREAERAFKSEHR
                     DVLLEPREEPAEGERREPGIGAAEDEPRRFSEAPMKRSTPELPRLLDARADCVIRLET
                     IEALEVGRVWAIQAEQLAGLGKPVYWFGFNDAENVWQPLGPDSSGACHWFCAALQLVN
                     RQGSIGETDFMRFSGGVQRVADALMSLPPALPPRGETLRNATELDRFCADVDVQIGIN
                     VVSREGQFEGRAIHAVAEKHGLRLGADGAYHMVDGEHSVFTVANLESGRFSPESLKGL
                     VTRGLTLVIDVPRVSNGGAAFDRMMKMATTLAGELGGDVVDDNRAPFGAEAATIIRNQ
                     IGQFQSRMADHDIPAGSPLALRLFSL"
     gene            complement(29444..>31236)
                     /locus_tag="PKN25_00455"
     CDS             complement(29444..>31236)
                     /locus_tag="PKN25_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNV89394.1"
                     /translation="AIATDVAPAVSALAGLVEVRDPALRALVDDFLSGAWAVERLEDW
                     LPLRAQLGPSTCLVGPRGQVLTRDALVHHAPDARTHGVIERQREIEGLSAELQAHEDE
                     AHLAHDALVVAESAASALQERINGLRRELQTIQAQVHAEQVEVLKLAQARARAQERRE
                     QLARDLEDIVHLESAEREHLTRAELEQARAAELAELQRERLDAATEVLREREHAVREA
                     RALEQSAARELQEARFSERECAGKLEDIARNQQLAGEQLERVVAELAARAAELDATDD
                     HRSAEALQEALALRGRREAALAARRDALAEAAAALKQVEELRLRTEHEAAPVRARVAE
                     LRLALQAAELAVAQFEERLVEARADEAALAPLLAAEPKESTLQREVARLAREIAELGA
                     VNMAALDELTTASERKGYLDAQTEDLLQAIDTLEDAIRRIDRETREQLQDTYNTVNRQ
                     FGALFPQLFGGGRAELVLTGEEILDAGIQIVAQPPGKKNASIHLLSGGEKALTAIALV
                     FSMFQLNPAPFCMLDEVDAPLDDTNTERYANMVKRMSAQTQFIFISHSKITMEFAQQL
                     VGVTMQEQGVSRVVEVDIEEALRLADPAAA"
     assembly_gap    31237..31336
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31337..33183)
                     /locus_tag="PKN25_00460"
     CDS             complement(<31337..33183)
                     /locus_tag="PKN25_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV89395.1"
                     /translation="MRLSKLKLAGFKTFVDPTTVLTPGNLVGVVGPNGCGKSNIIDAV
                     RWVLGETRASALRGESMQDVIFNGSTTRKPVSRASVELVFDNAEGRAAGQWSRYAEIS
                     VKRVLDRSGESTYYINNVHVRRKDVIDLFLGTGLGPRAYAIIEQGMISRIIEARPEEI
                     RGFLEEAAGVTKYRERRKETEGRLRDARDNLARLDDIRMELGERIVHLEAQAAVAARY
                     RELDAAHVEKQQLLWLVKRNEARAEQARVAASLNEASSRIEADSARLQELETSVESRR
                     DAHFEASEAVHVAQNDLFAASAEVARLETELQHLGEARRRLEARLAQLELDRGHWSSR
                     RETLAADRARWQELAENAALRAEHAEARHLEIADRLPELDSSRQGADATMAAARRELA
                     QTEQQLRVEETKRASALRALEALQQRRGRLEGERGGIVGPDERVLAEREARLEALQDE
                     LEVHQQELAAAQPRLPDAQAALKAALEHERAVQRRLTELRARRDALMQLQARVQSQGK
                     LGDWLERHGLDQLPPLWKQLQVAAGWDEAVQAVLRERLAALTSPDPALALAAARTVLD
                     ETPPESLAIALPARSGAPAERANSAQGPLSPQGRVTVATTATESSTAIAT"
     gene            33450..34643
                     /gene="dapC"
                     /locus_tag="PKN25_00465"
     CDS             33450..34643
                     /gene="dapC"
                     /locus_tag="PKN25_00465"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HNV89396.1"
                     /translation="MNPNLARLQSYPFEKLRALFAGVEPPAHLKPIRLSIGEPQHPTP
                     GFIRQTLADNLGGLSSYPATLGSDALRGAIAHWLERRYGLPKIDPASQVIPVAGTREA
                     LFAFAQCVVDGTKPGAKVLCPNPFYQIYEGAALLANAEPIFINNLPESDFGSDFGSID
                     EATWRDIQLVYVCSPGNPTGRLLTLDEWKTLFELSDRYGFVIAADECYSEIYFDDADK
                     PIGGLEAAWRLGRTDFRNVVMFSSLSKRSNVPGMRSGFVAGDAKILGDFLLYRTYHGC
                     ALNPAVQAASVVAWNDEEHVEENRRLYRDKFARITPMLQPWLPVRLPDAGFYYWVRTP
                     LPDTEFARRLQAEYNVTVLPGSYLARESNGVNPGAGFVRIALVADTHECVEAAGRIID
                     FCKTL"
     gene            34658..35479
                     /gene="dapD"
                     /locus_tag="PKN25_00470"
     CDS             34658..35479
                     /gene="dapD"
                     /locus_tag="PKN25_00470"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNV89397.1"
                     /translation="MHALQSIIDTAFENRASLSPTSAPAEIRNAVEEVIAGLDAGTLR
                     VAEKKDGQWVVNQWIKKAVLISFRLRDNEVVQAGGLNFFDKVATKFGDYTPEQFREGG
                     FRVVPPAVARKGSFIGRNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPVQAGPVIIEDNVFVGARSEVVEGVIIEENAVLSMGVYIGMSTKIYDRE
                     TGEITYGRVPAGAVVVPGSLPSACGKYSLYCAVIVKKVDAQTRAKTGINELLRGA"
     gene            35553..36725
                     /locus_tag="PKN25_00475"
     CDS             35553..36725
                     /locus_tag="PKN25_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNV89398.1"
                     /translation="MIFDNLFQLMAEKKASDIFITAGTPIHIKIQGHTMPINQQVMDP
                     LMIQRMLHEAITPAQQAVLERERELNLSFGRRDLGNFRVNVFWQRNSMAVVVRYIQSD
                     IPSIGALGLPPVLDDIVLEKRGLVLVVGATGSGKSTTLAAMIDHRNRNRSGHILTIED
                     PIEYLFKHRKSVVNQREVGIDTLSWGEALRNAMRQAPDCILIGEIRDRETMQAAISYS
                     QTGHLCLATLHANNAYHALNRIVNFFPLENRQLLYLDLAVALRCVISQRLVRKPDGKR
                     IPAVEILMNTRHIAELVERGELNAIKEAMEQSLAQGSQTFEQALYRLHHDNIITLEEA
                     LANADSPSNLHWLINNAQMQSDPMLTVNGNSAISGFEATNTEGASFQEFTLDLNNN"
     gene            36750..37907
                     /gene="dapE"
                     /locus_tag="PKN25_00480"
     CDS             36750..37907
                     /gene="dapE"
                     /locus_tag="PKN25_00480"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HNV89399.1"
                     /translation="MQTSPSSSSTLALACELIARPSVTPEDAGCLELVASRLAPLGFR
                     LERIDSGGVCNLWARRGIAAPVLCFAGHTDVVPPGPDSAWSSPPFAPTIRDGVLYGRG
                     AADMKSSLAAFVTAIERFVAAHPDHAGSIALLLTSDEEGIATHGTVKVVEALAARGER
                     LDYCVVGEPTSVQTLGDMIKNGRRGSLSGTLRVKGQQGHVAYPQLARNPIHLFAPALA
                     ELAAIDWDQGNEFFPPTTWQVSNIHAGTGANNVIPGVCEVMFNFRFGSVSSADELKSR
                     TCEVLDRHGLDYEIDWHLSGKPFITGRGRLVGALSAAIRETLGVETELSTTGGTSDGR
                     FIAEICAEVVEFGPVNASIHKIDEHIATDAIEPLSVVYERTLTALLVPGRL"
     gene            37967..38920
                     /gene="prmB"
                     /locus_tag="PKN25_00485"
     CDS             37967..38920
                     /gene="prmB"
                     /locus_tag="PKN25_00485"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNV89400.1"
                     /translation="MANQQHADDDNLALEQHDDHEHEHGPLAELVTVRDWLRYAVTRF
                     NRAGVFCGHGVHDSFDEAVWLILGSLALPLDRLEPFMDACIPSEERIGLLEAIEQRVD
                     ERVPTAYILGEAWLGDFRFTVDPRVIVPRSFFAELLEDGFAPWVEDPEAVGSVLDMCT
                     GSGCLAILMAHAFPNAHVSAVDLSEDALDVARINVADYGLEDRIELVHGDVFEGLEGR
                     RFDLILSNPPYVTAEAMEALPPEYLHEPQMALASGEDGLDVVRRLLAEAAEHLNPEGI
                     LAVEVGHNRAIVEAAFPDLPFEWLPTRGGDDMVFVLRRKDL"
     gene            38968..39426
                     /locus_tag="PKN25_00490"
     CDS             38968..39426
                     /locus_tag="PKN25_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV89401.1"
                     /translation="MASERPILLVEDNPDDEALTLHALSRNRITSPVVVAHDGVEAVE
                     YLFSTGSRAGQPLPPLPAVVLLDLKLPRIDGLEVLRRIRADQRTALLPVVVLTTSREP
                     LDLAQAYRLGANSYIRKPVDFDRFERAIGLIGHYWLELNESTETVARGAY"
     gene            complement(39535..39729)
                     /gene="iscX"
                     /locus_tag="PKN25_00495"
     CDS             complement(39535..39729)
                     /gene="iscX"
                     /locus_tag="PKN25_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNV89402.1"
                     /translation="MKWTEVQEIAIQLADAHPDVDPTRVNFVDLMNWTMALPDFDDDP
                     KRCGERILEGIQQAWIDEVA"
     gene            complement(39731..40072)
                     /gene="fdx"
                     /locus_tag="PKN25_00500"
     CDS             complement(39731..40072)
                     /gene="fdx"
                     /locus_tag="PKN25_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HNV89403.1"
                     /translation="MTQIIVLPHVELCPDGSVLEAAPGTTLCDALLEHGIDIEHACEK
                     SCACTTCHVIVREGFSSLNAAEDEEEDLLDKAWGLEPQSRLSCQAVVAATPLVVEIPR
                     YTINMAREGKH"
     gene            complement(40083..41951)
                     /gene="hscA"
                     /locus_tag="PKN25_00505"
     CDS             complement(40083..41951)
                     /gene="hscA"
                     /locus_tag="PKN25_00505"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HNV89404.1"
                     /translation="MALLQITEPGMSTEPHQHRLAVGIDLGTTNSLVATVRNGIAICL
                     PDEAGRTMLPSAVRYHADGGIEVGLGALKAQAVDPRNTIVSVKRFMGRGLKDVTHIEA
                     MPYDFEDSPGMVRLRTVQGVKSPVEVSAEILRRLRERAEASLGGALVGAVITVPAYFD
                     DAQRQATKDAARLAGLDVLRLLNEPTAAAVAYGLDNAAEGVYAVYDLGGGTFDLSILK
                     LSRGVFEVLSTNGDAALGGDDFDHRLFCWILDRAKISPPSLEDARRLQMKAREAKELL
                     TSCDEAPVHVRLASGEEVDLVVTREEFAEMTTHLVQKTLAPVRKALRDAGLAPDEIKG
                     VVMVGGATRMPHVQRAVANYFGQEPLTNLDPDKVVALGAAMQANVLAGNRADQDDWLL
                     LDVIPLSLGLETMGGLVEKVVPRNATLPIARAQEFTTFKDGQTAMAFHVVQGERELVS
                     DCRSLARFELRGIPPMVAGAARIRVTFQVDADGLLAVSAREMSSGVEASVLVKPSYGL
                     SDDEIAEMLRSGVDHAGDDMAARALREQQVEADRVVEATEQALGHDGHLLSAVESAEI
                     RSVIARLRELRAGTDNRAIKAGIDALARATDTFAARRMDNSIRSALAGHKVDELPI"
     gene            complement(41979..42512)
                     /gene="hscB"
                     /locus_tag="PKN25_00510"
     CDS             complement(41979..42512)
                     /gene="hscB"
                     /locus_tag="PKN25_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNV89405.1"
                     /translation="MSIDLTQDYFALFGLPRRYALDEGALEAAWHELQGRVHPDRHAH
                     LSDFEKRRSMQWATRVNEGFRVLRKPLARAQYLLELAGVDAALETNTAMSPEFLMEQM
                     EWREAVEEAREAAEVSELEDLHRRLLGHAREVRGHLAAQLDEHQDYEAAADTVRRLMF
                     IEKLQQEIDEALLALEN"
     gene            complement(42523..42846)
                     /gene="iscA"
                     /locus_tag="PKN25_00515"
     CDS             complement(42523..42846)
                     /gene="iscA"
                     /locus_tag="PKN25_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HNV89406.1"
                     /translation="MAVTLSESAARHVSNFIAKRGKGFGIRLGVKTSGCSGMAYKLEF
                     VDQTEDEDEVFESHGVKVVIDPKSLAYLDGTELDFVKEGLNEGFKFNNPNVKDQCGCG
                     ESFNV"
     gene            complement(42873..43256)
                     /gene="iscU"
                     /locus_tag="PKN25_00520"
     CDS             complement(42873..43256)
                     /gene="iscU"
                     /locus_tag="PKN25_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNV89407.1"
                     /translation="MAYSEKVLDHYENPRNVGSFGKEEEGVATGMVGAPACGDVMKLQ
                     IKVGKDGVIEDAKFKTYGCGSAIASSSLVTEWVKGKTLDQALDIKNTQIAEELALPPV
                     KIHCSILAEDAIKAAVADYKKKHEA"
     gene            complement(43323..44534)
                     /locus_tag="PKN25_00525"
     CDS             complement(43323..44534)
                     /locus_tag="PKN25_00525"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNV89408.1"
                     /translation="MLKFPIYLDYSATTPVDPRVAAKMIPWLTEHFGNPASRSHAYGW
                     EAEKAVEDAREQVAALVNADAKEIIWTSGATESNNLAIKGAAHFYQGKGKHLITVKTE
                     HKAVLDTVRELEREGFEATYLDVQENGLIDLEVLKAAIRPDTIVVSVMFVNNEVGVVQ
                     PIAEIGEICREKGIVFHVDAAQATGKVDIDLDRLKVDLMSFCAHKTYGPKGIGALYVR
                     RKPRVRLEAQMHGGGHERGLRSGTLATHQIVGMGESFRIAREEMAEENARVGKLRDRL
                     LAGLTDLEATFVNGDLAQRVPHNLNISFAYVEGESLIMAVKDIAVSSGSACTSASLEP
                     SYVLRALGRNDELAHSSIRFTIGRFTTEEEIDYTIKLLHDKIGKLRELSPLWEMYKDG
                     VDLDAVQWAAH"
     gene            complement(44592..45755)
                     /locus_tag="PKN25_00530"
     CDS             complement(44592..45755)
                     /locus_tag="PKN25_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNV89409.1"
                     /translation="MNFAPIYLDWNATTPLDPAVREAMLPWLGAAEPARFGNASSRHE
                     YGRQARAAVDEARARVAAAVGAHATEVIFTSGGSEANNLFLKGAAPNREPGVVAVSAI
                     EHPCVREPARQLRRAGWTLREIAVDAQGVIDPADWSAVLEARPGLVSVMLANNETGVL
                     QDVAALARAARAAGAWFHTDAVQALGKVAVDFRALGVHAMTLSAHKIGGPLGAGALVL
                     DKRVELAPLIAGGGQERGLRSGTENVAAIVGFGVACERAVARREGEGVRLAALRDELC
                     AALAGRGARIFSAAAPRLPNTVFFAVEDIDGETLVGRLDRAGFACASGSACSSANPEP
                     SRTLLAMGVERGLARAAVRVSLGRDTRADDVRSFIETFVRVTDELKNLASIGA"
     gene            complement(45764..46246)
                     /gene="iscR"
                     /locus_tag="PKN25_00535"
     CDS             complement(45764..46246)
                     /gene="iscR"
                     /locus_tag="PKN25_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HNV89410.1"
                     /translation="MRLTTKGRFAVTAMIDLAARQGDGPVTLAGIAERQSISLSYLEQ
                     LFGKLRRHKLVNSVRGPGGGYRLARAMDEITVADIIIAVDEPMDATQCGGKENCHDDH
                     VCMTHHLWSNLNQRIYAYLDSVSLEALVKHQVRPDADMTVLKSVRRRSRMPVHEIAAV
                     "
     gene            complement(46453..47220)
                     /gene="cysE"
                     /locus_tag="PKN25_00540"
     CDS             complement(46453..47220)
                     /gene="cysE"
                     /locus_tag="PKN25_00540"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNV89411.1"
                     /translation="MFSRLREDLASVRERDPAARSTLEVLTCYPGIHALMFHRLAHGA
                     WRRGWFWAGRFASHVGRLLTGIEIHPGAKIGRRVFIDHGMGVVIGETAEIGDDCTIYQ
                     GVTLGGTSLYRGTKRHPTLGRGVVVGAGAKVLGGFTVGDGAKVGSNAVVVKPVPAGAT
                     AVGNPARILDSDKDSERDAAREQKAEQFGFSAYGVTRDMDDPVAKALHGLLDHSVETD
                     RRLQAILARLEAAGIKLDDTVAPSDHFDPGRLSRMVD"
     gene            47424..48197
                     /locus_tag="PKN25_00545"
     CDS             47424..48197
                     /locus_tag="PKN25_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HNV89412.1"
                     /translation="MLQLFRRLLGIPPARPLVDESQWRRVEARLPFLDHLVPDDRLRL
                     REMALAFLGCKEFHGAQGLELDDDMLLAIALQACLPVLHIGLQAYEGWVGVIVYPGDF
                     LIPRRIVDEAGVLHEYEDEVLGEAWAGGPVLLSWFPGNDAPAGVNVVIHEFAHKLDME
                     NGDVDGLPRLRPGMSRRDWAKAFGEAYEAFCAEVDRGVDTTIDPYGAEHPGEFFAVVS
                     EVFFEDPLVLHRRFPAVYDQLVHFYGMDPAARAAAAAGG"
     gene            48199..48663
                     /locus_tag="PKN25_00550"
     CDS             48199..48663
                     /locus_tag="PKN25_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNV89413.1"
                     /translation="MSAPKRLLIVHHTQSGNTGRLAAAVLEGALRVSETETRLLRAFD
                     AGTADLLACDGLLLGTPENFGYMSGALKDFFDRTYYPCEGKLVGLPYAVFVSAGNDGT
                     GAVREIGRIANGYGWKTVAEALIVRREITPADLERARELGEAMAAGLAMGVF"
     gene            48870..49310
                     /locus_tag="PKN25_00555"
     CDS             48870..49310
                     /locus_tag="PKN25_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HNV89414.1"
                     /translation="MAITWILVANASLAKLYANLGPNKGLTLVKELIHPESRQKNGDL
                     VSDRSGANGSNGTGGGSMQPQTLPKQHEAKVFAQEIAQTLYHGRTSNAFKRAILVAPP
                     AFMGLLNAVIDGPTAQMVTDRFEKDYTKSTQDELGERLGSTIFL"
     gene            complement(49542..50102)
                     /gene="thpR"
                     /locus_tag="PKN25_00560"
     CDS             complement(49542..50102)
                     /gene="thpR"
                     /locus_tag="PKN25_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNV89415.1"
                     /translation="MAEAAADAGRAPNLRLFFALWPDAATRAGLHARARALHSECGGR
                     AMRRDTIHLTLAFLGETPVRQVERLRAVAAGVQGEGFALDLDRVGSWHRNRILWTGPS
                     MVPPALAALAQDLEGRLRADGCALEARAFSPHVTLVRNAHAAPREAQLAPLRWTVGSF
                     VLVASERSAAGAHYRTIGRWPLKRRG"
     gene            complement(50095..50589)
                     /gene="ispF"
                     /locus_tag="PKN25_00565"
     CDS             complement(50095..50589)
                     /gene="ispF"
                     /locus_tag="PKN25_00565"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNV89416.1"
                     /translation="MSIPFRIGQGFDVHALVPGRPLIVGGVDIPFGHGLLGHSDADVL
                     LHAVTDALLGAAGLGDIGRLFPDTDPAHAGADSRVLLREAFARVRAAGWEVVNLDATL
                     ICKAPRILPHAPAMVANLAADLEMDATAINIKGKTTEKLGFTGRGEGIAAQVVALIAR
                     AEHG"
     gene            complement(50586..51296)
                     /gene="ispD"
                     /locus_tag="PKN25_00570"
     CDS             complement(50586..51296)
                     /gene="ispD"
                     /locus_tag="PKN25_00570"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNV89417.1"
                     /translation="MQISHPRHFAIVPAAGSGSRMAAARPKQYLSLLGRPLIHHALSV
                     LCAAPAIDAVFVVLSVDDTEWATHDWSALGPKLRPLFCGGATRADSVLGGLRAIAGEA
                     AAGDWVLVHDAARPCLAPWHVDKLVRELAHDEVGGLLAVPVADTLKRADEHRHVCETV
                     PRDSLWQAQTPQMFRYAMLRRALEGAREVTDEASAIEAAGLRPRLVQGDATNLKVTYP
                     LDLHLAEWILENREGHNA"
     gene            51426..52250
                     /gene="amrB"
                     /locus_tag="PKN25_00575"
     CDS             51426..52250
                     /gene="amrB"
                     /locus_tag="PKN25_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNV89418.1"
                     /translation="MALASIRPAAVAGQFYPRDERVLRTQLAEMLSTAVAFESAPAPK
                     AIIVPHAGYMYSGPVAASAYALLAPLRERIRRVVMLGPTHRVAVRGFSLPAAQRFATP
                     LGEVPVARGDWEALQARDDVLVDDRPHALEHCLEVQLPFLQVCLERFEIVPVLVGDAS
                     PEATASLIERLWGGPETLILISSDLSHYLSYREAQWSDRAAVDQVLELRPGLDHEQAC
                     GATPINGLLLAARKHDLQAHLLDLRNSGDTAGDRSRVVGYTSIAFCEAEPPSHARH"
     gene            52237..52803
                     /gene="amrA"
                     /locus_tag="PKN25_00580"
     CDS             52237..52803
                     /gene="amrA"
                     /locus_tag="PKN25_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNV89419.1"
                     /translation="MPDTDFGTTLLALARTAIAHRLGRGPAPVIPDDPRLHEKGATFI
                     TLAKDGELRGCIGSLRRSRELGEDVIHNALAAAFEDSRFEPLAPEELGAVAIEVSILS
                     EPEFLDFFGEEELLAQLRPGEDGLIIFSGCHSATFLPQVWEQLPEPRQFLAALKHKAG
                     LPTNREVVELMAARYHVEKFKETEREPS"
     gene            52800..53924
                     /gene="amrS"
                     /locus_tag="PKN25_00585"
     CDS             52800..53924
                     /gene="amrS"
                     /locus_tag="PKN25_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNV89420.1"
                     /translation="MNTKPAPAAEVGSRHPARYWHRIDDGRYQCDLCPRLCKLHPGQR
                     GACFVRMREGDDMLLTTYGRSSGFCIDPIEKKPLAHFYPGSKVFSFGTAGCNLACKFC
                     QNWDISKSRDLDTLMDEASPAEIAETALRWGCHSVAFTYNDPVIFAEYAMDVADACHA
                     RGLKTVAVTAGYISECARRDFFSRMDAANVDLKGFTDDFYVKLCGAHLQPVLDTLVWL
                     RHETDVWVELTTLLIPGHNDSDSEVTALANWVRKELGAEVPLHFSAFHPDFKMDELPP
                     TPPATLARARRIALDAGLKHVYTGNVHDIEGDTTRCASCGEPLIVRDWYEIRRYALGN
                     DGHCPHCGSVLAGRFAAVGAAVGDAFGARRIPVAIHRHGT"
     gene            53943..54599
                     /locus_tag="PKN25_00590"
     CDS             53943..54599
                     /locus_tag="PKN25_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV89421.1"
                     /translation="MKLRNTLISIPAQGAWLDGLLAHAPDVRGLAILPQSSGHPALDS
                     GPRPVDVALQSRGFATLRVDLLTRQEALRDPDASYNIARLTERILAAVEWIAHQPPLA
                     GLQLGLVTQDTGCAAAIRAAVKAPETFAAIACVDGRADLAGAEPLRQLRCPVRFIVSP
                     GAPESAILEKAFSLLGGARHWLALPDTGDARQQEILQARAVTSWLEQHLAAAERTRVE
                     "
     gene            54620..55252
                     /locus_tag="PKN25_00595"
     CDS             54620..55252
                     /locus_tag="PKN25_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HNV89422.1"
                     /translation="MSAMSISSLGGGDAAWRQGTAMNAARPAGASGGPATAGAGGKAQ
                     EAGAGAADTLSEAEQRQVAELAERDREVRAHEMAHVAAGAGLVTRGASYTYQTGPDGQ
                     RYAIGGEVGIDTSPGRSPEESLSKAERIRAAALAPAEPSGQDRQVAAQASRMASDARM
                     EIARREFEQGGAGTEAAATRDGPGYRGMRAAQAYSAALAPGQGPTLSATA"
     gene            55411..58884
                     /gene="mfd"
                     /locus_tag="PKN25_00600"
     CDS             55411..58884
                     /gene="mfd"
                     /locus_tag="PKN25_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNV89423.1"
                     /translation="MSLSLDALLSRLATVKLPKPGGRLELEPLAGSADALAIAQLASR
                     GRMLVVITANPLDAQRLQDEIAWVAPGLRAHLLPDWETLPYDSFSPHQDLISERLSTL
                     YAISRGETDIVLVPASTALYRMAPPGFLAAYTFFLKQGEKLDVEQFKAQMALAGYAHV
                     TQVVSPGEFSVRGGLVDLFPMGSPLPFRIDLFDDEVESIKTFDPDTQRTVYPTKEIRL
                     LPAREFPLDDKGRTRFRSRFRETFEGDPTRASIYKDVSNGIAPAGIEYYLPLFFDETA
                     TLLDYLPAETPVLLHRDVPAAIAEFWKDTRSRHDLLKGDRSRPVLAPEQLFLTDEAFF
                     IALKNLPRLAIGAEVGAKAGAEPAAARTDALALPPPEVAVERKATDPLHKLKAFLDGF
                     DGRVLLLSDSPGRRETMAEFFAEYDVRPEATADFGAFLDSDARLALGVAPLARGFVLP
                     AARLAVLTETELYAATARSRVRRDARKAATMEGWLRDLSELKIGDPVVHVSHGIGRYL
                     GLLHMNLGEGDTEFLHLEYNGGDKLYVPVSQLHVITRYAGADPEAVDLHRLGSGQWEK
                     AKKKAAMQVRDTAAELLALYAQRAARPGHRFDFKQHDLEAFAEAFGFETTPDQQAAID
                     AVVADMKSGRPMDRLVCGDVGFGKTEVALRAAFIAVADGKQVVVLCPTTLLAEQHYQT
                     FADRFADWPIKLAELSRFKSAKEQAEALQLLAEGKVDIIIGTHRLLQKDVIFKRLGLV
                     IIDEEHRFGVRQKEALKQLRSEVDILTLTATPIPRTLGLAMEGLREFSVIATAPQKRL
                     AIKTFVQPSSRGLIREAVLREFKRGGQVYFLHNEVDTIDNMKDDLEELLPEARIVVGH
                     GQLPERELERVMRDFTQQRANLLLCTTIIETGINIPTANTIIINRADRFGLAQLHQLR
                     GRVGRSHHQAYAYLLTQPGAKPSAQAQKRLEAIAMMEDLGSGFYLAMHDLEIRGAGEV
                     LGENQSGEIQQVGFSLYTEMLKRAVKDLQAGKEPDLSQPLEVVSEINLHTPALLPTDY
                     CPDVQERLTLYKRLANCEMEEDLRALQEELIDRFGELPAQTAALIETHRLRMLVKDYG
                     VHKLDASDAQISVQFAKDAPIDPVKVIFLIQKDRSTKMAGPEKLVRRASLPNLKERVK
                     AVKDLLEAVKA"
     gene            58914..59879
                     /gene="corA"
                     /locus_tag="PKN25_00605"
     CDS             58914..59879
                     /gene="corA"
                     /locus_tag="PKN25_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNV89424.1"
                     /translation="MLINCIAYQHGRKLADIPVEDISEYIQQPETFVWVALKDATAEE
                     LAVMKEEFGLHELAVEDANHRHQRPKVEEYDDELFVVMHLIEEREGKLEVGEVHVFVG
                     TNYILSVRNNHARDFLGVRARCEREPKMLAKGPGYVLYALMDAVVDGYFPLIDKLETE
                     VEALEERIFTKGAGNTNMRRLYRLKRKVTLLKHAVLPLMEAAGKLHSGRVPEVCAKSR
                     HYFRDVYDHLARIDASLDSVRETIGTAIQVNISLVTIDQTEVSKRLAAWAGIFAVATA
                     FAGIWGMNFNHMPELEWEYGYFAGLGIIVSASTFLWWRFRKARWL"
     gene            60175..61512
                     /locus_tag="PKN25_00610"
     CDS             60175..61512
                     /locus_tag="PKN25_00610"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNV89425.1"
                     /translation="MSFFDDLARSLERNRITRRQALWLLGAGAAAGLSGCATSPVTGE
                     TILVGMSEAQEKQTDAQVAPHQFSQDLGAIQDEAVNRYVAGIGQRMGTLTHRPQMPYS
                     YRVLNANYVNAYTFPGGAMGVTRGILADLDDEAQLAALLGHELGHVNARHAAQRQGQN
                     LVAQAALAGLNVAAQSSDWGGLMSMGGQIGASALLAGYSREHEREADALGQEYLVKAG
                     YPATGMVRLHQLLVAEEKSAPSLLQTMFSTHPMSSERMQAAQAAADARYRISNSLDAR
                     RERFMDSTASLRRIRPTIDACKNGETAMAARQYPKAQAEFQTALARTPRDYASNLRMA
                     QCLQAQGQTAKAVDYADNAREIYPQEAQAYKLAGVLALQQRDAGRAYQNLDRFDRLLP
                     GDAGITFLKGISLEGMGNRQAAAQHYAAYLRQSQQGNAAQYSYNRLKAWGMVK"
     gene            complement(61557..62933)
                     /gene="ccoG"
                     /locus_tag="PKN25_00615"
     CDS             complement(61557..62933)
                     /gene="ccoG"
                     /locus_tag="PKN25_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNV89426.1"
                     /translation="MREAKLEFYRKKGKIHAKAVQGRFNSLRWAMVWLTQVVFYGACW
                     LPWTTETGTRQAILFDIVDEKFYLFDLVLWPQDALLLAVVLILAATALFFTTAIAGRL
                     FCGFACPQTVYTSIFVWIEARIEGDHLARLRLDQGPMSARKLLIKSVKHGIWALVAAW
                     TAISFVGYFTPIRALLPAIVGLQTGPWETFWLLFYASFTYLQAGLAREAVCQHMCPYS
                     RFQGVMIDPDTANVAYDVKRGEPRGALRQSGDKGDCIDCGICVQVCPTGIDIRDGLQY
                     QCIDCGLCADACDEVMDRIGTPRGLIRFMSERELAGQAQVAPAWSRPRTLVYVGLLLV
                     FAALGVWTLSERVLLRVEVHRDRGALMQETSAGRIENSYTLKLINMEETTRQFRIGVS
                     GLPGLRIEGAGEFTVGPGQVQSVTLTLSSPFEGMPSGVQPIVFETVALHDASLAVREK
                     GTFILPFR"
BASE COUNT         9887 a        22348 c        20753 g         9846 t
ORIGIN      
        1 cccgaagtta acgtggtcgt cttccgcgat cggccagccg agccgaatac cggcgccggt
       61 cgagacggtc ttgtacggcg acaccgagtc gagttcgtcg gcgtcgtagg tgcggtggta
      121 caggtcccaa cccaggctga tgccgtccac cgtgtagtag gggttggtga aggacagcgc
      181 gagcgtgcgg ctggactgac tggtgttgag gccgagcgtc agggcgttgc cgctgccgaa
      241 caggttctgc tgcgagatcg acgccgacag cacgatgttc tcggagctgg agaaaccggc
      301 gccgagcatc aggtttccgg tcgggcgttc cttgacggtg aagttcacgt cgacctggtc
      361 ggtcgtgccc ggtatcgccg gcgtctcgac ggtcacctcg tcgaagaagc cgaggcggtc
      421 gatgcgcgta cgcgagcggt tgatcgccgc cgcgtcgtac cagccccctt ccatctggcg
      481 catctcgcgg cgcaccacct catcgcgcgt cttggtgttg ccgccgacgt tgatgcggcg
      541 cacataaacc cgccgacccg gatcgacgaa tacggtgaag gccacctcgc gcttgacctt
      601 gtcgacttcc ggcgcggcat tgacgttggc gaaggcataa ccctcgttgc caaggcggtc
      661 ggtgatcgcc ttggtggtct ccgtcaggcg ttcgcgcgag aaggtttcgc ccggtcggat
      721 cttcgtcagc gcctggtatt cggcctcggg aaggatcagg tcgccggtga agcgcacgcc
      781 ggtgacggtg tacttctcgc cctcggtgat gttcaccgtg atgtagatgt cctgcttgtc
      841 cggtgtgatc gagacctggg tggagtcgat gttgaagtcg aggtagccct ggttcaggta
      901 ccaggagcgc aaggtctcca ggtcggccga gagcttctgg cgggagtact ggtcgttctt
      961 ggtgtaccag gtcaaccagc cgggcgtggt gagctgcatc tcgtcgagga gatcttcttc
     1021 ttcgaaagcc ctcgcgccga cgatgcggat ctggcggatc ttggccacgc ccccctcgtc
     1081 caccttgaag ttgatgccga cacggttgcg ttcgagcggc gtgacggtcg tcgtgaccgt
     1141 ggccgcgtac ttgccgcgcg agaggtactg gcgcttgagt tcctgctccg cgcgctcgag
     1201 caacgagcga tcgaagatgc gcgattcggc gaggccgacc tcgcgcaagc ccttcttgag
     1261 cgcgtccttg tcgaactcct tgaccccgtc gaaggtgatg tcggcgatcg ctgcgcgctc
     1321 gtccaccacc accacgatga cgttgccttc ggtctcgatg cgaacgtcgc tgaagaaacc
     1381 cgtggcgaac agcgcgcgga tcgcatcggc ggcctgcgcc tcggtgaagg tctcgccgac
     1441 gcggaccggc aggtagttga acacggtgcc cgcctcggtg cgctggatgc cctcgacgcg
     1501 gatgtccttg accacgaagg gatcgaaggc gagggccggc gctgcggcaa aaagggccac
     1561 caggagcccg ggcaggagct tgcgattcat gcgggagttc agccggaaat aagacgattg
     1621 agatcgttgt agaaggcgaa tgccatcaac atcaccagga gggcgaggcc gatctgctga
     1681 ccgacctcca tgatgcgctc cgggatcgga ccgcccttga tgatttcgac cgtatagtac
     1741 aacaagtgcc ccccatccag aaccgggatg ggcagcaggt tcaggacccc caggctgatg
     1801 ctgatcaagg ccacgaactt gaggtagtgc gccaagccca gctgggcggt ctgacctgca
     1861 tagtcggcga tcgtgaccgg tccggagagg ttcttccagg acacttcgcc ggtgagcatg
     1921 cggccgatca tcttcaggct cagcacgctg gtctcccagg tctgccgaac cgcgcgcgcg
     1981 aggccttcga gcacaccgta gcggatctcg ccgaacatct cgatgccgcc cgtcgcggct
     2041 tcgcccaccc ccacgccgat gcggccgatc cgcgccccgc cctcctcggc cgcgtccggg
     2101 gtcaccacaa gccctaccac cccgcccgca cggtcgatct cgaagtcgag cgcgcgtccg
     2161 ggcgcctcgc gcaccaggcg cacgaggtcg gcccaggccg cgaccgccgt gcccgagatc
     2221 gccagcacgc gatccccgac ctgcagtccc gcacgttcgg cagcgctgcc atccgcgatc
     2281 ctgccgatca ccgccggaag cgccggacgc caggggcgca ggccgaggcg cgcgatcagg
     2341 tcggtgttgc cctcgtcgat cgccacgccc gagagatcca tgcggcggaa ggcatcgacg
     2401 tcctccaggg tgcgtacgcg cagcaccacc tcgcggttgt cgagcgcgtg gcgcagcagc
     2461 acccagcgca ggtcctgcca gctgcgcacg ggctcctcgt ccaccgcgat cacgaggtcg
     2521 ccctcgcgca cccccgccgc ctgcgcgatc gccggcgtgt cggacaacgc cacgcgaggc
     2581 ttgagttcct cggtgccgcc gacaaagagg ccccagtaga gggcgatcgc gagcaggaag
     2641 ttggcgagcg gcccggcggc gacgatcgcg aaacgccgat acacgctctg gcggttgaag
     2701 gcgcggtgca gctcgggtgc cgctacgggc gcctcgcgct cgtcgagcat cttgacgtag
     2761 ccgccgagcg ggaacgcggc gagcacccat tcggtgccgt cgctgccggc ggtatggcgc
     2821 agcagcggct tgccgaagcc gatcgagaag cgcagcacct tgaccccgca ccaacgtgcc
     2881 acgaggtagt ggccgagttc gtggacgagg atgagcaggc cgagggcaag cgcgaaggga
     2941 accaggtaat cgaagagagt catcggtcgc ggcgaagtcg ggagatctcg tcccgcgcga
     3001 gttcgcgggc gtgcgcgtcg gcggccaata ccgcctccag cgaatcgacc gcgcccggcc
     3061 ggctgcgttc caggctcgct gcgatcacgg cggcgatgtc gaggaagccg atgcgatggt
     3121 cgaggaaggc ggcgaccgcc tcctcgttgg cggcgttgag cacggcgggc gccgtgccgc
     3181 cggcgcgcag cgcgtcgaag gccagcttca ggcaggggaa gcgggcgaag tccggacgtt
     3241 cgaaatcgag gcgggcgatc tcgaacaggt cgagcgggcg cacacccgcg tcgatgcgct
     3301 cgggccaggc catggcgttg gcgatcgggg tgcgcatgtc ggggttgccg agctgtgcga
     3361 tcaccgagcc gtcggcgtag tcgaccatcg agtggatcac gctctgcgga tgcacgacca
     3421 cctcgatcat gtccgccggg gcgccgaaca gccaatgcgc ctcgatgacc tccaggccct
     3481 tgttcatcat ggtcgcggag tcgaccgaga tcttgcgtcc catgacccag ttgggatggg
     3541 cgcaggcctg ctcgggggtg acattcgcga gcgaatcgat cggcgccgtg cggaaggggc
     3601 cgccggaggc cgtcagcaac accctgcgca cgccggcgcg cgcgggttcg cgcgcgtagc
     3661 cggcgggcag ggcctggaag atcgcgttgt gctcgctgtc gatcggcagc aggaccgccc
     3721 cgcttgcggc cactgcatcg atcagcaagc ggcccgacag caccagggcc tccttgttgg
     3781 ccagcagcac cttcttgccc gcgcgcgcgg cggccagggt cgggcgcagg ccggcggcgc
     3841 ctacgatcgc ggccatcacc atggccacct cgttggcggc ggcgacacgc tcgagcgcct
     3901 cttcaccgca tagcacctcg gtgtcgcgac ccgctgcgcg caatcccgcg cgcaggcgtt
     3961 cggcagcggt cgcatcgccc atcaccgcga agcgcggcga aaaacgcagg cactgctcga
     4021 gcagcttgtc atcctggcgc tgcgcactga gcgcgaagac ctcgaaacgg tcggggtgac
     4081 gggcgacgac atcgagcgtg ctttggccga tcgagccggt tgcgcccagg acggtcaggc
     4141 gctgcggtgc ggggtcaggc atggggagaa tccgttgcgg aaggagcacg gcggaagccg
     4201 tggaggatca gcgcgcgagc cacaccgcgc cgagcgccac gagcgggagc gtcgacgtca
     4261 ggctgtcgat gcggtcgagg atgccgccgt ggccgggcag gatgttgcca ctgtccttga
     4321 ggccggcctg acgcttgagc agcgactcga agaggtcgcc gacgatgctc accgcggtcc
     4381 acgccagcag gacgccggcg atcaccggcg cgccgccctc gaattccgcg ccgcgcatcg
     4441 cgagcacgcc gaagccgaag gccagcaccg ccagcacgcc acccgcggcg ccctcccaag
     4501 tcttgcccgg gctgatcgcc ggagccagct tgtgacggcc gaaggcgcgg ccgctgaaat
     4561 aggcggcgat gtcggccacc cagacgacgg ccatggttcc gagcagcacc ccggggccca
     4621 tcgtgcgcag ctgcatcatt gccagcgcgg tcggcacgag cacgacgaag ccgaccagcg
     4681 aagccagcat cgggttggac aaggcccact tgccgcgcag ccacatcggc acgacgaaga
     4741 tccagaataa cgcgctgaag acatagagcg gcaccacggc cgcagcaccg ccgagctcgc
     4801 cgctgcccag ccccgcgctc caacccagga acgcggatgc ggcggcaaag gcgaccgccg
     4861 tggacacgcg caacccgcgc tcgaagcggg cgagcgcgcc ccactcccac gccgccgcac
     4921 cgcacaagac tgcgcagagc acgagccaga ccgatgccgg cagcaggaac accgccgcca
     4981 gcaggccgac cagcaaggcg attgcggtga ggacgcgatc cttgagcatg gaggccctat
     5041 tccccggcgt cggccttgag ctgctcgctg gtgcggccga agcgccgctc ccgcccctga
     5101 taggagccga tggcctgccc gagcgcaccg tcgtcgaagt cgggccagag cacgtcggtg
     5161 aagtagagct cggtataggc gagctgccag agcaggaagt tgctgatgcg cttctcgcca
     5221 cccgtgcgga tgaagagatc gggctccggc gcgaagctca tcgacagctc ggaggcgatc
     5281 tcatcttcgg tgaagcgttc caggcgcccc ggttcgcgcg ccatcaggcg cgacatcgca
     5341 tcgagaatgt cccagcgacc gccgtagttg gccgcgacgg tgaagttgag tccgccattg
     5401 ccggcggtga gcgtctcggc ctcgcggatc atgtccacca gccgcggatc gaacttcgag
     5461 aggtccccga tcacgcggaa gcgcatgtcg ttgtcgtgca tgcggcggat ctccttctgc
     5521 agcgacttca gaaagagctg catcaggaaa gaaacctcgt cctgcgggcg gcgccagttc
     5581 tcggagctga acgcgaacac ggtgaggtac tccaccccgc gatcgacgca gccgcgcacg
     5641 gtggcgcgca gcgcctcgac accgcgcgca tgccccgcga cgcgaggcag catgcgcttg
     5701 cgcgcccagc gcccattgcc gtccatgatg acggcaatgt gcttcggcac ctgcgagacg
     5761 tcgggaatgt cgcgggtgga actcctgaac agccctccga tcatgctccg cctcctgtcg
     5821 tgcccgttga cgatacagcc gatccgcgca cgcggatcag atctgcatga gttcctgctc
     5881 tttctgggcg agcagcttgt cgatctcggc tacatagcgg tcggtgagct tctggatctc
     5941 gtcctcgccg cgacgctcgt cgtcctcgga gatcgccttg tccttgaccg catccttgag
     6001 ctgctggttg ccgtcgcggc gcaggttgcg gatcgccacc ttggcggtct cgccctcgtg
     6061 gcgcaccacc ttggtgaggt cgcgacggcg ctcctcggtg agcgcaggca tcggcacgcg
     6121 cagcagttcg cccatgttcg cggggttgag gccgaggtcg ctgtcgcgga tcgcgcgctc
     6181 gaccttgccg agcatcggct tttcccaggt ctgcacgccg atcgtgcggg cgtcgatcag
     6241 ggtgatgttg gcgacctggt tcaccggcac catcgaaccg tagtactcga ccatcacgtg
     6301 atcgagcagg ccggtgtggg cgcggccggt gcgcaccttg gcgaggtcgg tcttgagcgc
     6361 ctcgaccgac ttcagcatct tctgctcgat gttcttcttg agttcggaaa tcatgcaagc
     6421 cctctccgga ggtcaggaat ggaccagcgt gccttcgtcc tcgcccatca ccacacgctt
     6481 gagcgcgccg ggcttgaaga tcgagaacac gttgatcgga agcttctggt cgcggcacaa
     6541 cgcgaacgcg gtcgagtcga gcacctgcag gttgcgcccg atcgcctcgt cgaagctgat
     6601 gcgatggtag cgctgcgcct gcgggtcctt cttcggatcc gcagtataga cgccgtccac
     6661 cttggtcgcc ttgagaacga tctgcgcacc gatctcggcg ccgcgcagcg ccgcggcggt
     6721 gtcggtggtg aagaaggggt tgccggtacc ggccgcgaag atgaccacgc ggccttcctc
     6781 gagatgacgg atcgcgcgcc cgcggatgta cggctcgacc acctggtcga tgcgcagcgc
     6841 ggactgcacg cgcgcttcga gccccatgcg tcgcatcgcg tcggcgagcg ccatcgcgtt
     6901 catcaccgtg gccagcatgc ccatgtagtc ggcggtggcg cggtccatgc cggaggccgc
     6961 gcccttcatg ccgcggaaga tgttgccgcc gccgatcacc acaccgacct ggacaccgag
     7021 acggctgatc tcggcgacct cggacacgat gcggctgacg acctcctcgt tgatgccgta
     7081 ggcgtcgtcg cccatcaggg cttcgccgga gagcttgagc aggatgcgct tgtaggcggc
     7141 ggacacgttc ttctccttac ttcttggcgg cagcggcggc ctgttcggcc acttcggcgg
     7201 cgaagtcggt gaccttcttc tcgatgcctt cgccgacgat gaacagcgtg aagccggcga
     7261 ccgaggcgcc cttggccttg agcagctgct cgatggtctg cttgccgtcc tcggccttca
     7321 cgaagacctg ggccagcagg gtcacttcct tgaggaactt ctgcacggtg ccgtcggcga
     7381 tcttttccag catcgcttcg ggcttgttgt cggcgcgggc cttctcgatc gcgatgcgac
     7441 gctcggcatc gatcaggtcc tgcgacacgc ccgaggcgtc gagcgccttc ggcttggacg
     7501 cggcgatgtg catcgcgatg tccttgccga gctgctcgtc gccgccgacc acgtccagca
     7561 gcacgccgat cttggcgccg ccgtggatgt aggagaacag cttgcccttg gcctcgatgc
     7621 gggcgaaacg gcggatcgac atgttctcgc cgatcttgcc gaccagcgcg gtgcgggtcg
     7681 actcgacggt gccctcgccc agcggcagcg ccgacagggc agcgacgtcg gccgggctct
     7741 tggcggcgac gaggccggcg cagttctgca ccagggccag gaagtcgtcg ttcttggcga
     7801 cgaagtcggt ctcgcagttg acctcgatga tcgagccgag cttgccgtcg gccgagatgt
     7861 cgatgccgac cacgccttcg gcggcgatgc gggcggcggc cttggaggcc ttgttgccga
     7921 gcttgacgcg caggatctct tcggccttgc ccatgtcgcc ggcggcttcg gccagcgcct
     7981 tcttgcattc catcatcggc gcgtcggtct tctcgcgcag ttccttgacc atgcttgcgg
     8041 tgatttccgc catgctagct cctgaatagg ttcggtttga cattcgaaac gcggatgccg
     8101 cgcagcgcac cggagggcca tcggcgcgac atcgggaatt gtgcggggcg cacgttgcgg
     8161 gattcgcaag cgccacccgg attgcaaaag gggccccgcg ggcccccttc gctgaggatc
     8221 aggcctgttc ctgcgagcct tcttcctcga cctcgacgaa ctcgtcgccg ccggcgtcga
     8281 cgatctcctg cagcacctgg ctgcggccct gcagcacggc gtcggccacg ccacgggcgt
     8341 agaggcggat ggcgcgcgag gagtcgtcgt tacccgggat caggtaatcc acgccttcgg
     8401 gcgagtggtt ggtgtcgacc acggccacga ccgggatgcc gagcttctgg gcttcggtga
     8461 tggcgatctt gtggtagccg acgtcgatga cgaacagcgc gtcgggcagg ccgcccatgt
     8521 ccttgatgcc gccgatcgac ttctgcagct tctcgagctc gcggcgcacg gtcagcgcct
     8581 cgcgcttgga caggcgctcg atcgagccgt cttccatcat cgcctcgact tccttgaggc
     8641 gcttgatcga ctgcttgacg gtcttgaagt tggtcagcat gccgccgagc cagcgctcgt
     8701 cgacgtaggg catgcctgcg cgctgggctt cctcggcgat gatctcgcgc gcctggcgct
     8761 tggtgctgac gaacatgatg ttgccgcggt tcgcggcgag cttgcgcacg aactccatgg
     8821 cttcgttgta cttcgccatc gtcttttcga ggttgacgat gtggatcttg ttgcgctggc
     8881 cgaagatgta gggggccatg cgcgggttcc agaagcgggt ctggtgaccg aagtggacgc
     8941 cggcttccag catctgacgc atcgtgacgg acatgtgttg ctccaaactg tacaagggtt
     9001 gaacctccgc ctcgcggatg ggcgaccggg aaaccggttc accacgctgc cccccgggat
     9061 ttgcgcgtcc cccgccccgg ccggagccgg aacgcaggat gtcgagcgag ccccggaagg
     9121 accctgtgcc gcaaggcgtg cggattttgc ctgcacatgc agacaagccc gcgatgttat
     9181 cacgccggcc gcatcctgct caaggctgaa gcggattgaa acccaggccg acacaagcct
     9241 cgcgggtacc cgccgcggaa atggcgaagg catcggttgg catgcggttt gcgcggatcc
     9301 cgggtttgct gcggcgcggc gcttgcgata gccttcccgc tttcccgccc cgacacagaa
     9361 cacacaacaa tgagcattgt catcaagacc cccgaagaga tcgagaagat gcgcgtggcc
     9421 tgccggctcg ccgccgaggt gctcgactac atcgagccgt tcgttgtccc cggcgtgacc
     9481 accggggagc tcgaccgcct ctgccacgac tacatggtga acgtgcaggg caccatcccg
     9541 gccccgctcg actacgcacc gcccggctac aagccctacc ccaagtcgat ctgcacttcg
     9601 gtcaatcacc aggtctgcca tggcgtaccg ggcgagaagg cgctcaagaa gggcgacatc
     9661 gtcaacctcg acattacggt gatcaaggag ggcttccacg gcgacaccag ccgcatgttc
     9721 ctgctcggcg gcgactcggc gtgcagcatc gtcgcccgcc gcctgacgcg gatcacctac
     9781 gagtgcatgt ggctgggcat cgccaccgtc aagcccggcg gccgcctcgg cgacatcggc
     9841 gccgcgatcc agaagcacgc cgaaggcaac ggcttctcgg tggtgcgcga gttctgcggc
     9901 cacggcatcg ggcgcaagtt ccacgaggaa ccgcaggtgc tgcactacgg caaggccggc
     9961 accggcccca cgctcgagcc cggcatgatc ttcaccatcg agccgatgat caacgccggc
    10021 aaggcggcga tctccgagct gcccgacggg tggaccatcg tcaccaagga ccgcagcctg
    10081 tcggcgcagt gggagcacac ggtggtggtc accgagaccg gtgtcgaggt gctcaccctt
    10141 tccgccgggt gcccgccccc gcccgagctc gtcgctgccc gtttcgcaac ggctggctga
    10201 agaatccgtc gcaggcccgc acatgaacga cgccctcacc gacgagctgc acacccttgc
    10261 caccacggca cgccaacgtc tcgtcgaggg cacggcgcag ctgcgcgacg cctatcaggg
    10321 gcggccgcag acctccatcc tgctcaaggg ccgcgctcga ctggtggatc acgagctcgt
    10381 ccgcctgtgg acggcgtgcg cgatgcccgc ctccgccgcg ctcgtggcgg tgggcggcta
    10441 tgggcgcggc gagcttttcc ccgcctcgga cgtcgacctg ctcgtgctgc tatccacacc
    10501 gcccgacgag gcgctgaagg cgcgcctgtc ggcgctggtc ggcgcgttgt gggactgcgg
    10561 gctcgaggtc ggtcatgcgg tgcgcacggt cgacgaatgc atgatcgccg ccgccgacga
    10621 catcaccgtg cagaccaacc tgctcgaggc gcgtctgctc gccggcgacc cggcgctgtt
    10681 tgccgagctg agcgcgcgct accgcgaagc gctcgacgtg cgcgccttct acaaggccaa
    10741 gctgctcgag caggaacaac gctacgcccg cttcaacgac acgccctacg ccctcgagcc
    10801 caactgcaag gagagccccg gcgggctgcg cgacctgcag atgctgggct ggatcgcacg
    10861 cgcggccggg cttggccaca gctggcgcga cctcgcccgc caccgcctca tcaccggcag
    10921 cgaagccggc gaactgcgcg cgatcgagcg cttcctgcag cacatccgca tccgcctgca
    10981 tcatctcacc gggcgcgcgg aggaccgcct gctcttcgac caccaggaaa agctcgcccg
    11041 catcctcggc atcgaggcca cgcccacccg gcgcgcctcc gaggtgctga tgcagcgcta
    11101 ctacgtcacc gccaagatgg tgatgcagct caacaccgtg ctgctgcaga actacggcag
    11161 cgtgatcttc cccgaccgcg gcgccgcgat cgtggtcaac gcgcgcttcc aggtcgtgcg
    11221 cgagctgctc gacatccgcg acgaggacgt cttcgagcgc cacccttccg cgctgctcga
    11281 atgcttcctg ctgctgcagc agcgctccga gctcaagggc atgacggcgc gcaccctgcg
    11341 cgcgctgtgg atccaccgca atcaggtcaa tgccgcgttt cgcgccgacc ccgcgcaccg
    11401 tgtcctcttc ctgcagatcc tgcagcagcg ccgcggtatc gtgcgggtgt tccgcaagat
    11461 gaacgagtac ggcatcctgt cgcgctacat cccggcgtgg cggcgcatcg tcggccagat
    11521 gcagcacgac ctcttccacg tctatacggt cgaccagcac atcctgatgg tgctgcgcaa
    11581 cgtgcgccgg ttcacgatgg gagagcacgc gcacgaatac ccgctgatga cgcggctgat
    11641 catggccttc gagcgccact ggctgctcta cgtcgccgcg ctcttccacg acatcgccaa
    11701 gggccgcggc ggcgaccact ccacgctcgg catggccgac gcgcgcgaat tctgcaccca
    11761 gcacggcatg gacgcgaacg acaccgcact ggtcgtgtgg ctggtcgagc aacacctcac
    11821 gatgtcgcgg gtcgcacaga aggaagacac ctccgacccc gaggtcatcc agcgcttcgc
    11881 cgacgtcgtg cgcgacgagc gtcgcctggt cgcgctctac ctgctcaccc acgccgacat
    11941 ccgcggcacc agccccaagg tgtggaacgg ctggaagggc aagctgctcg aggatctgtt
    12001 cttcgccacc cagcgcctgc tgcgcggcgc caccgccaag gaggcgctcg gcctcgacgg
    12061 ccgcctcgag gacgcccgcc gccagttgcg cttccacggc ctgcgccccg gcgtcgagga
    12121 cgcgctgtgg gcccggctcg acccggtgta cttcatgcgc cactccgccg aggagatcgc
    12181 ctggcacacc cgcttgttgt actaccgcac cgaatccgac gagccggtgg tgaaggcgcg
    12241 cgtggccgag gacgaggacg gcgtgcaggt catggtgttc acccgcgacc agaaggacct
    12301 cttcgtgcgc ctgaccggct tcttcgggcg catgggcttc accatcgtcg acgccaaggt
    12361 gcacaccacc cgccacggct acgcgctcga cagcttcatg ctgcaggacc cgggcgaaca
    12421 ggccgcctac cgcgacatcg tgatcctgat cgagcacgag ctcaccgccc gcctgcagaa
    12481 accggggctg cccgagcgcc cgagtcgcgg gagaatgtcg aggcaggtca agcacttccc
    12541 gatcagcccg gaggtcagca tccgtcccga cgaggccggc cgccatcacg tgctctcgct
    12601 caccgccgcc gatcgccccg gcctgctcta cgacgtcgcc gcggtgctcg ccgagcacgg
    12661 catccgcctg cacaccgcca agatcgccac cctgggcgag cgcgtcgagg acaccttcct
    12721 gctcaccggc aacgtgctca accaggacgc gcaggtgctg aggatcgagc gcgagctgct
    12781 ggagcggctg caggtctagc cgcgctcgcg gcgcgctccg gatgcgctcg cggcgcgctc
    12841 agtgcccctt ccccgccagg cgatccatgg cggcgtagag caggcccgag aacaggaagg
    12901 tcacgtggat gcccaccatc cagccgagct ggcggtcggt gaggttgtgg gcgttcatga
    12961 aggccttgag cagttcgatc ccggagatcg ccacgatcga gccgatcagc ttgagcttga
    13021 ggtcggagaa gccgatgtgt cccatccact ccgggcggtc gcggtgggtg tgcaggtcct
    13081 ccatcttgga cacgaagttc tcgtagccgc tgaacatgat gatgatcagc aggttcatga
    13141 tcagcgccac gtccaccagc gacagcacgc cgatgatcag ctcgctgccc gaggcgctcc
    13201 agatctgcgc gaacagcccg aacagctcct tggagaactt caccagcagc aataccatcg
    13261 ccagcacgag gccgaaatac accggcgcca tcagccagcg gctggtgaac agcacgtgct
    13321 cgaacccgct ctcgatccgc ttcatcgcat cgcgctcctc gaccggtcga tcagacatgg
    13381 cacagcccct ccagcacggc gcgcacgcgc accatggact cctgcaacac cagctcgatg
    13441 tcgtcgaagt ggatgccgtg cgcgctcgac gagcgccccg cggcgtagtt caccaccacc
    13501 ccgatcgccg cgtagggcag ctcgagctcg cgcgccagcg ccgcctcggg catgccggtc
    13561 atgccgacga tgtcgcagcc gtcgcgctcg aggcggtcga tctccgccgc ggtctcgagg
    13621 cgcgggccct gcgtgctcgc gtacaccccg ccatccatcg cgctgtcgcc ggccgcggcg
    13681 atcgccgcga gcagacggtg gcgcagggtc tcgtcgtagg gctcggtgaa gtcgatgtgg
    13741 cgcaccggcg tgtcgccgcc ctcgaagaag gtgctcttgc gcccccaggt gtagtcgatg
    13801 atctggttcg ggacgatcag cgtgcccggc gccaggtcgg cgcggatgcc tcccaccgag
    13861 gccaccgaga cgatgccggt ggccttggcc tgcttgagcg cccagatgtt ggcgcggtaa
    13921 ttgaccaggt gcggcgggat ggtgtggccg tagccgtggc gggcgaggaa gacgaccggc
    13981 tcgtcgcaga cgcgcccgaa ggcgagtgcg ccggagggct cgccgtaggg tgtgcgcacg
    14041 acctcgcggc gctcgatctc catgttcgac agttgggtga gtccgctgcc gccgatgatc
    14101 gcgagcatgg tgaagagacc tcctttggct gaggcgcgcg attctagcac gcccctcaga
    14161 aatccatgct gcgctgccac agcacgtgct agaatcctcg ccctttcaaa gacttcgccg
    14221 ccctcctcca tgtcccgcgc cattcgcaac atcgccatca tcgcccacgt cgaccacggc
    14281 aagacaaccc tcgtcgatca actgctgcgc cagtccggca ctttccgcga aaaccagcag
    14341 gtcgccgaac gcgtcatgga ctcgggagac atcgagaagg aacgcggcat cacgatcctg
    14401 tcgaagaact gcgccatcca gtatggcgat acccacatca acatcgtcga caccccgggc
    14461 cacgccgact tcggcggcga ggtcgagcgt gtcctgtcga tggtcgacag cgtgctgctg
    14521 ctggtcgacg ccgtcgaggg cccgatgccg cagacccgct tcgtgacccg caaggcgctc
    14581 gcgctgggac tcaagccgat cgtcgtcatc aacaagatcg accgcccggg cgcacgcccg
    14641 gactgggtca tcaaccacac cttcgacctc ttcgacaagc tcggcgccac cgaggaacag
    14701 ctcgacttcc cggtgatcta tgcctccggc ctcaacggct tcgcagtcat caacgagggc
    14761 gacgagcgca aggacatgcg cccgctgttc gaagccatcc tcgagcacgt gcccgcgccg
    14821 gaagtcgatg ccaacggccc gctgcagatc caggtctgct cgctcgacta ctcgacctac
    14881 atgggccagc tcggcatcgg tcgcatcaag cgcggcagcg tgcgcccgaa ccaggaagtc
    14941 gtcgtcatgt acggcgaaga gaaccgcggc aagggcaagg tcagccagat cctcaccttc
    15001 gagggcctcg agcgcaaacc ctccgagctg gccgaggccg gcgacatcgt gctgatcgcg
    15061 ggcatccagc aggtcaacat cggcgtcacc ctgtgcgacc cggagtgtct ggaaggcatg
    15121 aagccgatcg cggtggacga gcccacgctg accatgaact tcatggtgaa cacctcgccg
    15181 ctggccggtc gcgagggcaa gtacgtcacc agccgccaga tccgcgagcg cctggaaaag
    15241 gaactgctca agaacgtcgc cctgcgcgtc aattacaccg gcgactcgga cgtgttcgag
    15301 gtctccggtc gtggcgaact ccacctcacc atcctgctcg agaacatgcg ccgcgagggc
    15361 tacgagctgg cggtgggacg cccgcgcgtg gtctacaagg aaatcgacgg cgtgaagtgc
    15421 gagccgtacg agatgctcac cgtcgacgtc gaggaagacc accagggcag cgtcatggaa
    15481 gagctcggcc gtcgccgtgg cgaactccag gacatgcagc cggacggcag gggccgcgtg
    15541 cgcctcgaat accgcatccc ggctcgcggc ctgatcggct tccagggcga gttcctcacc
    15601 atgacgcgcg gcaccggcct cgccagccac gtcttcgacg actacggccc gatggccggc
    15661 gccatggccg agcgccgcaa cggcgtgctg atctcgcaga acgacggcga cgcggtggcc
    15721 tacgcgctgt ggaacctgca ggaccgtggc cgcatgttcg tcagcccggg cgaggcgctg
    15781 tacgaaggca tgatcatcgg catccacacc cgcgacaacg acctcgtggt caacccgatc
    15841 aagggcaagc agctcaccaa cgtgcgcgcc tcgggtaccg acgaagccgt gcgcctgatc
    15901 acgccgatcc agctcaccct ggaatctgcg atcgagttca tcgccgacga cgagctggtc
    15961 gaaatcacgc ccaagaacat ccgcctgcgc aagcgtcacc tgaaggaaac cgaccgcaag
    16021 cgcgctgcca aggccgacgc gtcctgatct caggccgctc gaacgaaaga cgccccgccg
    16081 gttctgcccg cggggcgtct tgccatccgg gcgcttgcca caaacgcagc gcacggtttg
    16141 caaatgcccg ctttcctgct atcacggaag taccacaaat gaatggagcg cgcgccgcgg
    16201 acacgcggta atgcgcgccc agaaccgaca gagagcttcg ctgcgcaaag cgcagagcgc
    16261 gggtgcagtt gtgcccgacg atctgcacag cgcgtccctg cgggtgtccg cttcggatgc
    16321 cttcgtccct gccgatccga cagagcagga gaacgaacat gatgcgtcca tcatcgcagg
    16381 tggtgttggc ggccgttgtc ttcattgccg ccatgctcgc cccgcccctt cccctggcaa
    16441 tcgctgatgc gcccgccgcc tcgcccgcgt cgacgccggc aagaattcat gcccaggacc
    16501 tcccatatta cgaggcttgc gcgcgggaag gcttgaacga gtccgaatgc gccggacggc
    16561 tgatctggtt caaggccact gccggcaacg agcgcttcca cacctacgtg ttccagcagc
    16621 gggtgggcgt gctgatcgac tggttccgcg tgctgcgcag cgacgagcgc ggcgaccgct
    16681 tccgcgcctg gggcatcatc aacgatccgg cctgctgcgt gcccggcagc cccgactgcc
    16741 cggcgaagag cctggacgag acctacggtc tcgactggtg tccgggcgac gaggaactcc
    16801 tcccccatgt cgggcgcagc ggctaccgcg atccggcctg cgacttccgc gatgcagcgc
    16861 tgaagcccgg cgatgtgcat ggcccagccg accagcgcca cgacgcctgc gacctgcgtt
    16921 tcggcacctc gaccggcgca ctgggcatcc gcaagtttcc gaacccgcgc ttcgaccgcg
    16981 cagcttggtt gaagctcaac ggcagcccgg gcacctggga aggctataac cggccgatcg
    17041 ccgccgccgg cggccggggc gaggcggccc gctcccacct ccaggacggt gccatcgagc
    17101 cccccttcct gatcggcacc tcctgcggtt cctgccacat cgccttcgat ccgctcaacc
    17161 ccccggctga cccggcgcgc ccggcatggg agaacatcaa ggggctgatc ggcaaccaat
    17221 acacgcgcgt ctccgagatc atggtctcgg gcatggcatc gaacaccctc gaatggcaga
    17281 tgttcgccca tgccaggccg ggaaccagcg acacctcggc gatcccgacc gaccaggtca
    17341 acaatcccgg cacgatcaac gcactgatca atctcgccaa gcgtccgacc ttcgccaacg
    17401 agtccgtgat caagtggcgc aagaccgcct cctgcgccaa gggcgtcgac gagacccggt
    17461 gctggtgcga gccgggccgc aacggcaaat gctgggagaa gtccctgcac aaggaagccg
    17521 tacaccacat cctcaagggc ggtgaggact cgatcggcgc gctcgaagcc atccagcgcg
    17581 tgtacttcaa catcggctcg tgctcggagc agtgctgggt caatcacctt gcagacctgc
    17641 gccagctcga tccgcaacaa cgcggctttg gccagacgcc cttcgacatc ggccagtgta
    17701 ggcgggactg cccgaacttc cgcgccgtcg aggaccgctt gggaaacatt ctcgacttct
    17761 tcatgtcggc cgaaggccat gccaccgacc tgcgcgaggc acgcgagaac acgctgcggg
    17821 ttgccaaccc cggcgcgcgt tacaccgatc aggacctgat cgccgatctc gaacgcacgt
    17881 tcggagcggg tgcggtcgcg cgtggccgtg aagtgttcgc caatggctgc gcacgctgcc
    17941 actcgagtca gcccgcggcc gcgactggcc agcttgccgg cctcgatttc cacaagctcg
    18001 acctcaagac ggggctgcgc gaggactggc tgggcaacga tggccccacc gcagtgtccg
    18061 aggtcggcac cttccgctgc cgcgccctgc actcgaacca catggccggc cacgtctggc
    18121 aggaatacgg ctccgaaacc ctgcgcgcac agccgcccga ccccaacgtc aaggaacccg
    18181 gcgacggtgg ccgcggccac taccgcaaca tctcgctgct caacctgtgg gcgcacgccc
    18241 ccttcctgca caacaacgcg ctcggccccg agctttgcgg caaaccgcac aacgccgcca
    18301 acgacttcta tgcccagcgt ccgcgctacg tcgatgcgag caatgtcaag ctgttgcccg
    18361 ccgagcgcca gcccgcctgc ttcgagtacg acccgagcgt cgaaggccga ttcaagctgt
    18421 acaccctgtc gatgcaggcg ctgctcaatc ccgcccagcg catccccaag gtgacgctgc
    18481 tgaacgagga cgtgaccctg cgcctcggcc cgcggctatg ggacggcacc gacaaggaac
    18541 gcctgctcgg tttcgaactg acgatcccga gcgagatcga cggccgcggc gtgaccgccg
    18601 gcacgctggg caacttccag cacaaggcct tcatcgtcga tctcgtgcgc gcgaagaccg
    18661 cgcccgacga gctcgagcgc agcctcgcca agcgcctggg caaggagagc gcgaggaagg
    18721 tgcttgccga cctccaggcc gtcgcgcggg aagtgaccgg caagccgaac ggcctggtcg
    18781 aggcgctgcg cgcgcgcccc tacctgatca agcaggtgta cagcagttgc accgccgagg
    18841 tcgagaacgc gggccaccgc tttggcgagg acttgtccga ggccgacaag aacgccctga
    18901 tcgccttcct cgcaacgctt tgagcatggg ggacttcatc atgacaagga tctcgcactg
    18961 gccgtttggg gcgctcgccg tctgcatgct ggccgccatg gcggttacgg gatgcggcct
    19021 gttcgacgac gacggggagc gggcgccgga gatcgccttc gccccgcatg ccgaaggcta
    19081 tgccggcaag cccgatttcg cctggctgga acatcgccat ccgctgtcga cggccgacct
    19141 gtacaagatc acgccgcaga acatcgcgca gctcgaccag gagcaggtcg accagatcta
    19201 tgcgcgcctg agcgccgggc cgatccccga cggcgcgttc gacggcgacc tgttcttccc
    19261 gcgcggtggc agcggcaagc tgcgcctgtc cgagatcgcc ggcggcggcc tgaagggctt
    19321 gctggtcaat gtggcgggcg gcaagctcga cctcgttggc gagatgttgt ggaagggcaa
    19381 ggtgttctac cgcgacgagc gcctgctgcg taaccgcatc gaggatctca ccaccttgcg
    19441 caagctcggc ctcgtcgagg aaagcgcgga caccccgctg cagaagctca atgtcggcgg
    19501 caaggaccag tggctgctgt tcccggcgaa gctctactgc ggccagagcc tgctcgacgg
    19561 ccggcgcgaa tccatcatca tcgactacgc cttcaccgat gaactgccgg gctaccggcg
    19621 catgcccgac ctgctcgcgg gccgcaacgg cttcgcgatc cgcgacgaga tccgcatggt
    19681 ctacccgggc ttctaccttg gtcgggccta catggaccgc gccttcgtgg tgaacttcgt
    19741 cctccacaac gcgcagatga tcgcggcgag tcgcgaagcg ttcctgaaga ccggccaggt
    19801 cgatcaggat tgctggagcg gcacccagca gcgcttgatc gccgccgcgg cagggggtgg
    19861 ctgagaacgg tccgcgcgtg gttttgcggg cggcggctgc gtccgcggcc cgcaagatcg
    19921 cgcaggggaa cgagcatgag actgtctcgt cctgtctccc gccatgtgct cccggtgctg
    19981 ctgggcgcgc tgttcggcag cgtgttcatg gctccggcca atgccgccga ggtcgaccgc
    20041 gcaccgtcgc cgcacgggcg gtcgctgttc gatcgcctgc tcgagcgcgg ggcgaacggc
    20101 gcgcagcagg tgcccttccc gttccccgca ctgctccaac gcgtgcgtgc gcagctcgat
    20161 ggcgaggacc cgaacggcgg actggcgatc gtgctgatcc cgctcgggcg ctcgctgcag
    20221 cgccacgccg caggagacgt cgatgccttc cgttttccgc gtgtggtggc cgcggtcacc
    20281 ggtgagccgg caagcgacgc acccgcggga cacctctacc tgaaggaccg cttgtacgtc
    20341 gcgcaccacg aacgtgccga ggcgctcgag gtgatcagct acaacgagga tgcgggtcgc
    20401 ttcgagttcc agctcgtgct cgactaccgc gccggggcaa ggccgcgggt ggtcgccgcg
    20461 aaccgtacgc tctgcctcgc ttgccaccag aacgaggcgc cgatcttctc gcgcccgtcg
    20521 tgggacgaga ccagcgccag cccgccggtc gccgcacgcc tggctgcgac cggcctcgac
    20581 tatcagggtc tgccctggcg tcacggcgtc gatgtgcctg aagcgatcga cgccgcgacc
    20641 gcacgcgcga atcgcctcgg cgtggcccag cgcctgtggc gggacggctg cgccctcgcc
    20701 accccgcagg ccggcatcga ctgccgtgcc caggcgctgc tgcacgcgct gcgccagcgg
    20761 ctgatcggca ccggggcgcc ggtcgcgcgc gcggatgcca cgaaccgcct ggtggatccg
    20821 ctgctcgcca actggcaggc gcgttggccg gacggcctcg tcattcccga tccgcaaatc
    20881 cccaaccgcc agcccttcgc cggcatcgag ggcgggattg cgcccccgag cgatgccgag
    20941 ctcgtgcgct atgcggacat tccggcggtc ttcgatccgc tcgcgctgcg ggcgccgatc
    21001 gagcactggc gcggacgcga cccgggcgac gtgatgcgct tcatccacct gctcgggacg
    21061 ctgttctcgc gcgccgacct tgcgcgcatc gagcgcgccc tcgcgcgcat gccacagcct
    21121 gcagcgcggg agatcgacct gagctgcacc ctgcgccggg ccaccgacgc aacgcggctc
    21181 gacctcgact gcgcgcgcgg atccgaggtc cggctgcagg cgcgcatcag gctcgacggc
    21241 gggcgcagcg ccgacggcat cctcgaccgt ctcgagttgt ccgggtcgag cgcgcacacc
    21301 ggtgtgacgc tggccatcgc gaaggaccac gacccggcat ccggctggcc gatccgcttc
    21361 gcgctcactg ccaaggcggg tggtccgatc cgcgccagca gtggggaagc catcaccacc
    21421 ttgcggctcg gtggcgacga cggcagcgcc cacctgccgg ggtcgccgag cgcggccgaa
    21481 gcggtcgtgg cagccctcga actcgtcgat gacctcgagc cgctcgggca cgccgtcgag
    21541 gccctcgcgc aggcaaatgt cgccggtcag gacgatgcgc tgacgggcgg cccggcacgc
    21601 cggatggccc tgctcgcgcc gctgctacgc gggctcggcg aggacgttcc tgccccgccc
    21661 gccgccgcag cgcctccacc cgcgttgatg gccgatgtca cccggtcccg cacgacgcac
    21721 tggcccgccg acctgcagcc cttcctgcgc cagtgcagcc agtgtcatgc cgagaacacc
    21781 gccttcccgc ccggcttcct gcacggcgac gaagcgcagg tacgcgccaa tctcgcgacc
    21841 tgtgcgcaac gcatgctcca tcggctggac atgaaccggc tgccgcccgc agcccgctcg
    21901 aaaaccccga tgccgccgcc cgctgcagcg catgccggcg ccttcctgca gtcgcccgac
    21961 ctggcggcga tgcgcgatct gctcgagcac gtgctgcagg caagtggcgt acgcagcgcc
    22021 gacgtgcttg cccatccgta cgccaccctc gcgccctgcc gagtcccgac agggtgatca
    22081 gcgctccgac agcgcaccgc accgtcttgc agatccgagg tcaccgtgaa cacgcccgag
    22141 tccgtcccca caacgcatcg cctgcgcctc gcgcgcacgc tgctccgcat cctcgtgctg
    22201 ctgccctggc tgctgctcct gctcgcgggc ggctatgccc tcgtgcggtt cgtgccggac
    22261 gagccggtcg tccatgcaga cccggtggag cacttcaagt acggctcgac cggcggcgag
    22321 cgcgcatcgg gcttcccgta ctggatctgg caggccttgc cgaccgtgtg cgcagaacat
    22381 ctgcccggtc ccggctacgc gtcgctcggc ctgattttcg aggaaggccg cgacctgccg
    22441 gtgggcgtgt cgaagcggcg ccacctcggc atcgatcgcg tcttcctcaa ctgcgccgct
    22501 tgccacgccg gcctcgttcg cgacacgccc gctgcgcccg cacgcctggt cgtcggcatg
    22561 cccgcccatc gcttcgacat tctcgccttc cagcgcttct tcttcgaatg cgccgccggc
    22621 ccgaccttct cgcgtgagtt catcgtgccg gagatcgagc gcctgagcgg cggactcggg
    22681 ctcgtcgatc gctacctcgt ctatccggtg gcgatcgcgt tgatgcgcga gcgcctgctg
    22741 atgctgcgcg cgcgctttgg cttcgtgtcc gcgcaagcgc cctgggggcc gggtcgggtc
    22801 gacaccttca acgccgcaaa ggtcctgttc aattttccgg tgcaacgact tcctgcccgc
    22861 gagcgcctcg gcgccgccga ttttccgtcg atctggaacc agcgcaagcg catgcaacgt
    22921 gacgatggcg agcgcatgga actgcactgg gacgggaaca acacccacac cgaggagcgc
    22981 aacaagagtg cagccttcgg taccggcacc acgcccccca ccatcgacct ggcggcaatc
    23041 tcccgggtcg aggcctggct gctcgacctc ggcccgccgg cgtggccgct gccggtggat
    23101 aagacactcg ccgcgcgcgg cgcggcgctg tatgcgcgct attgcgccga ttgccacggc
    23161 gcgagcggca gcgacttcgc agggccgaag gtcggccatg tcaccccgat cgcggacatc
    23221 gccaccgacc ccgcccggct cgattcctac acccgggatc tggcggtcaa tcaggcgacc
    23281 ctctatgcgg gctatccgca tcgtttccgc catttccgca agacctgggg ctacgccaac
    23341 atgccgctcg acggcatctg gctgcgcgcg ccctacctgc acaacggctc cgtgcccacc
    23401 ctgcgcgatc tgctcgagcc cgccttcgct cggccgacat gctttgtgcg cggcggcacg
    23461 ctgatcgatg cggtgcgggt cggcttcgcc agcgctgccg atcctgcgca atgcgccgcg
    23521 ccgacgggca gggcccagac cgcaaccgat cacttcctgt tcgacaccac gctgccgggc
    23581 aacggcaaca gcggtcacga aggcccggcc tatggcaccg atctgcccgc cgcggacaag
    23641 gatgcgctgg tcgagtacct gaagaccttc tgatgcgagg agtgccgcca tgaaattcgc
    23701 cccctgcacg aatccggggc cgcgccccac ccggctcctc ggccggcgcc gcaacaccgg
    23761 ccgcagcctg ctcgttgcgc tgctcgtgct gctcgcgctc ggcgccgccg gcggcgcctt
    23821 cgcctggtac aagttctttc gcgaggaagc gcagccggaa tggatcacca gcgatcccga
    23881 catgcgcttc aagtacggct cgatcggtgc cgaacgcgat gccggcatcc cctactggat
    23941 cttctacgtg ctgccgcgga tgtttcccga caagctgccg ggcccgggcg gctacgccgc
    24001 cttcggcgtg gtgtgggaag aaggccagga gctgccggtc ggcttctcga agaaggtggt
    24061 gggctttccg cgcgtggcca acaactgcgc gtcctgccac accacgagct accgcaccca
    24121 ggccgatgcg gcgccgactt tcgtgccgac cggccccaat cacacgctga acctgtgggc
    24181 cttcttccgc tttctggtcg actgcgcgaa agacccacgc ttcaacgccg acaacctgat
    24241 ggccgagatc aatctggtca ccgacctgtc cttcatcgac cgcctgctct atcgcttcgt
    24301 gatcatcccg atcacgcgca agcgcctgct cgagcgcgaa cagcagttcg cctggctgta
    24361 ccgcgacgat ttcccgccct ggggccgcgg ccgcgacgat gcgatgaacc tgaccaagta
    24421 cttcatgatc cgctggccga tggacgacag tttcggcccg accgacatgc cttcgctgtg
    24481 gaacctcggc aagtaccgcg ccgatcaggg catgcgcatg aacttcgccg gcgacagcca
    24541 cgacgcgtat tcggtggtga tcgactccgc gctcggcctg ctcggcgcgc cacccaagga
    24601 caacgccgag ttcctcggcg aggtgcgctg gctcatcgaa tacgtttcgg cgaaacgtgc
    24661 gccgccgtat ccgttcgcag tcgataccgc cgctgtagcg cgcggcaagc aggtgttcga
    24721 cacgacctgc gccggctgcc acgcatcggc gcgcaccgga acggtgatcc cgcttgccga
    24781 ggtcggcacc agcgcggagc gcatcgcgac ctggaacgaa cgcgcggcgc gcgaagcgaa
    24841 tcaggtggtg gccgggatgg gcatcgagcg cccgggcctg gtcgaagccc cgctgaccgg
    24901 ctacgtcgcc gccttcctcg acggcatctg gctgcgcgcg ccctacctgc acaacggctc
    24961 ggtgccctcg ctgcgcgacc tgctcgaacc gcccgcgcag cgccccaccc gcttctggcg
    25021 cggttatgac gtctatgatc cggaccgggt cggcttcgtc acccacggcc ccgaggccga
    25081 acgcatcggc accgtgcacg acgtcggggc ccgtggtggc agcaaccgtg gtcacgcgtt
    25141 cggaaccact ctccccgcca cggacaaggc ggacctgctc gaatacctca agacgatgtg
    25201 aaacgggcga cccgcgggcc gcccgtttcc gacccgcctc gacgcgcgag acgggactta
    25261 cttggctgcc ttcgccgcct tgccttcggc gccgaagcgc ccgctcaggt aggccgcgaa
    25321 gtcgccacca gtttccggat ggcgcaaccc gaactcgatc gtcgccttca ggtagccgag
    25381 cttcgaaccg cagtcgtagc gcacgccgtc gaactggaag gccagcacgg cctcctcctt
    25441 gagcagcgat gcgatcgcgt cggtgagctg gtactcgcca ccggcgccgg gcttcaacgc
    25501 acggatgtga tcgaagatgc gcggggtcag gatgtagcga ccgaccacgg cctgggtcgt
    25561 cggtgcttcc tcgggcttgg gcttctcgac gatgccggtc atgcgctgca cctggccctt
    25621 gtcggattcg gtgctgacga tgccgtagct gcgggtttcc tcgcgcggca cattcatcgt
    25681 gccgagcacc gagcagcggt gatagttgta cacatccacc atctgcttca acaccggcgt
    25741 gccatccttg ttgtcgagca ggtcgtcggc gaggatcacc gcgaaggcct cgtcgctcac
    25801 cacgtggctg gcgcaacgca cggcatggcc gaggccgagc gcctcggact ggcggatgta
    25861 gatacagttg acgcccttcg gcaccgtctg gcgcacgatc tcgagcagct gcttcttgcc
    25921 gcgcgcctcg agctcggtct cgagctcgta ggccttgtcg aagtggtcct cgatcgagcg
    25981 cttggtgcga ccggtgatga agatcatgtc ggtgatgccg gcggcgaccg cctcttcaac
    26041 ggcgtactgg atcaagggct tgtcgacgat cggcagcatc tccttcgggc tcgccttggt
    26101 ggcgggcagg aagcgcgtac ccatgccggc aaccgggaaa atcgccttgg tgactttctt
    26161 catttttatt gctctcccat gaatagaggg ggttgcgacg caacagcggg cgcgatcatg
    26221 cggcaagcgt gcgcggtttg catcaagcgt ttgtatccgt ttcgttcagc agcgcgcgca
    26281 ggccgtcctc gtcgaggatc gcaacgccga gcgcctgcgc cttgtcgagc ttggagcccg
    26341 cctcggcgcc ggcgaccacg tagtgcgtct tcttcgacac cgagccggcg accttgccgc
    26401 cgcgggcctc gatccgcgcc ttggcctcgt cgcggctcag ggtcggcagc gtgccggtga
    26461 gcacgaaggt cttgcccgcg agtgcgccgg ccaccgagcc ctgcggctcg ccctcggccc
    26521 aatgcacgcc ggcggcacgc aactgctcga tcacctcgcg attgtgcggc tcggcgaaga
    26581 actcggcgat gcatttcgcc acgatgggcc ccacgtcggg cacctgctgc agggcatcga
    26641 cgtcggccgc gatcagcgcg tcgagcttgc cgaagtggcg tgcgaggtcg cgcgcggtgg
    26701 cctcgccgac gttgcggatg ccgagcgcga agatgaagcg cgccagcgtg gtgccacggc
    26761 tcttctcgat cgccatcagc agattctgcg ccgacttctc gcccatgcgc tcgaggttgg
    26821 cgagcgcgag cacgccgagg cggtagaggt cgaccggcgt cttcacgatc gccgcgtcca
    26881 caagctgatc gaccagcttg tcgcccagtc cttcgatgtc catcgcacgt cggccggcga
    26941 agtgaagcag cgcctgcttg cgctgggccg ggcagaacag cccgccggtg cagcgcgcga
    27001 ccgcttcgtc ttcgccgcgc accacatgcg aaccgcacac cggacaggtg gggaaccggt
    27061 cgagcagcac gaagcgcggc agttcgccgc cgcggcgctc caggatcggc gccaccacct
    27121 cggggatcac gtcgcctgca cggcgcacga tcacccagtc gccgatgcgc acgtccttgc
    27181 ggtcgatctc gtcctggttg tgcagcgtgg cgttggtgac cgtgacgccg ccgacgaaga
    27241 ccggctcgag gcgcgccacc ggggtcagcg cgccggtgcg gccgacctgc acctcgatgt
    27301 cgcgcagcag cgtcaccgcc tcctgagcag ggtacttgtg cgccaccgcc cagcgcggct
    27361 cgcgggtgac gaagccgagc tgctgctgca gcgcgagcgc gtcgaccttg tagacgacgc
    27421 cgtcgatgtc gaagggcagc gtgtcgcgca gtgcgccgat gcgtgcgtgg aaggccgcca
    27481 gcccctctgc gccgcgcacc tgcgcgcggt ggccgcacac cggcaggccg aaggcggcga
    27541 gcgcgtccag cacctcggta tgggtcgcgg gcagcgtcca accggcgacc tcgcccagcc
    27601 cgtaggcgta gaaggacaag ggccggcgcg cggcgatgcc ggggtcgagc tggcggatgc
    27661 tgccggcggc ggcgttgcgc ggattgacga agatcttctc gccggcctcc gcctggcgcg
    27721 cgttgaggcg ctcgaagtcg tcgcggcgca ggtagatctc gccgcgcacc tccagcaccg
    27781 gcggcgcctc gccgagcagg cgcagcggga tcgccttcac cgtgcgcacg ttgctcgtga
    27841 cgtcctcccc ggtctcgccg tcgccgcggg tagcggcctg cacgagcacg ccgtcctcgt
    27901 agcgcaggct gatcgcgagg ccgtcgaact tgagctcggc ggcgtaggcc accgccgcat
    27961 cgtcctcgtc gaggccgagc gcgcggcgca cgcgcgcatc gaacgcgaac gccccgctcg
    28021 cctcggtgtc ggtctcggtg gagatcgaca gcatgggcac gcggtggcgc accgtggcga
    28081 actgcgcgag cgccttgccg cccacccgct gcgtcggcga gtcggcactg cgcagcgccg
    28141 ggtacgcccg ctcgatggcc tgcagctcgc ggaacaggcg atcgtactcg gcgtcgggaa
    28201 tcgtcggcgc gtcgagcacg taataggcat ggtcgtgacg cgcgatctcg gcgcgcaggg
    28261 ccgctgcgcg tgcggcggcg tcggccgcgg tctcgccgcg gccgtcttcg gcgaacaggt
    28321 ccatcacaag gagaacaggc gcagggcgag cgggctgccg gcggggatgt cgtggtcggc
    28381 catgcggctc tggaactggc cgatctggtt gcggatgatc gtcgccgcct cggcgccgaa
    28441 gggcgcacgg ttgtcgtcga ccacgtcgcc accgagttcg ccggcgagcg tggtcgccat
    28501 cttcatcatg cggtcgaagg cggcaccgcc gttgctcact cgcggcacgt cgatcaccag
    28561 cgtcagcccg cgcgtcacca ggcccttgag gctctcgggc gagaagcgac cggactcgag
    28621 gttggccacc gtgaacacgc tgtgctcgcc atcgaccatg tggtaagcgc cgtcggcccc
    28681 gaggcgcagg ccgtgcttct cggccacggc gtggatcgcg cggccttcga actggccttc
    28741 gcgggagacg acgttgatgc cgatctgcac gtcgacatcg gcgcagaagc ggtcgagctc
    28801 tgtggcgttg cgcagggtct cgccgcgcgg cggcagcgcc gggggcaggc tcatcagcgc
    28861 atcggccacg cgttgcacgc cgcccgagaa gcgcatgaag tcggtctcgc cgatcgagcc
    28921 ctggcggttg accagctgca gcgcggcgca gaaccagtgg caggcgccgc tgctgtcggg
    28981 accgagcggc tgccagacgt tctccgcatc gttgaagccg aaccagtaca ccggcttgcc
    29041 cagcccggcg agttgctcgg cctggatcgc ccacacacgg ccgacctcga gcgcctcgat
    29101 ggtctcgagg cggatcacgc agtcggcgcg cgcatcgagc aggcgtggca actcgggcgt
    29161 gctgcgcttc atcggcgcct cgctgaagcg gcgcggctcg tcttcggcgg caccgatgcc
    29221 gggttcgcga cgctcgccct cggcgggctc ctcgcgcggt tcgagcagca cgtcacggtg
    29281 ctcggacttg aaggcccgct cggcctcgcg tcggtggcgg cgctcctgcc atttgttgta
    29341 gcccaccacc agcaccaccg cggcgatgcc ggcgccgatc agcgcgatct gaagttcgct
    29401 gtccatttgg atcccagaat tgctccgtgt agcttgtcct gcgtcacgct gcggccgggt
    29461 cggccaggcg cagcgcctct tcgatatcga cttccaccac ccgcgagacg ccctgctcct
    29521 gcatcgtcac ccccaccagc tgctgcgcga attccatcgt gatcttgctg tggctgatga
    29581 agatgaactg cgtctgtgcg gacatgcgct tgaccatgtt cgcatagcgc tcggtgttcg
    29641 tgtcgtccag cggcgcatcc acctcgtcga gcatgcagaa cggggccgga ttgagctgga
    29701 acatcgagaa caccagcgcg atcgcggtga gtgccttttc cccgcccgag agcagatgga
    29761 tcgacgcgtt tttcttgccc ggcggctggg cgacgatctg gatgccggcg tcgaggatct
    29821 cctcgccggt cagcaccagc tccgcccgtc cgccgccgaa caactgcggg aacagggcgc
    29881 cgaactgccg attgaccgta ttgtaagtgt cctgaagctg ctcgcgcgtc tcgcgatcga
    29941 tgcgccggat ggcatcttcc agggtgtcga tggcctgcag caggtcctcg gtctgcgcgt
    30001 cgaggtagcc cttgcgctcg gaggcggtcg tgagctcgtc gagcgcggcc atgttgaccg
    30061 cgccaagctc ggcgatttcg cgcgccaggc gggcgacttc gcgctgcagg gtggattcct
    30121 tgggctcggc ggcgagcagc ggcgcgagcg ccgcctcgtc ggcgcgggcc tcgacgaggc
    30181 gctcctcgaa ctgggcaacc gcgagttcgg cggcctgcag cgcgaggcgc agctcggcca
    30241 cgcgggcgcg gaccggcgcg gcctcgtgct cggtgcgcag gcgcagctcc tcgacctgct
    30301 tgagcgccgc agcggcctcg gcgagcgcat cgcggcgggc ggcgagcgcg gcctcgcggc
    30361 gcccccgcag cgcgagcgcc tcttgcagtg cttcggcgct gcggtggtcg tcggtggcgt
    30421 cgagctcggc ggcacgcgcg gcgagctcgg cgacgacgcg ttcgagctgc tcgccggcga
    30481 gctgctggtt gcgtgcgatg tcctcgagct tgcccgcgca ctcgcgctcg gaaaacctcg
    30541 cttcctgcaa ctcgcgcgcg gccgattgtt cgagcgcgcg ggcctcccgc acggcgtgtt
    30601 cgcgctcgcg cagcacctcg gtggcggcgt cgaggcgttc gcgctgcaac tccgcgagct
    30661 cggccgcgcg cgcctgttcg agctcggcgc gggtgaggtg ctcgcgctct gcgctctcga
    30721 gatggacgat atcttcgagg tcgcgggcga gctgctcgcg acgctcctgc gcgcgggcgc
    30781 gtgcctgcgc gagcttgagc acctcgacct gctcggcatg cacctgtgcc tggatggtct
    30841 gcaactcgcg gcgcaggccg ttgatgcgtt cctgcagcgc gctcgcagcc gactcggcca
    30901 cgacgagcgc gtcatgcgcg aggtgggcct cgtcctcgtg cgcctgcaat tcggcggaga
    30961 gcccctcgat ctcgcgctgg cgctcgatca cgccgtgggt gcgtgcgtcg ggggcgtggt
    31021 gcaccagcgc gtcgcgggtc agcacctggc cgcgcgggcc gaccaggcag gtgctcggcc
    31081 ccagctgcgc gcgcagtggc agccagtcct ccagccgctc caccgcccac gccccggaaa
    31141 gaaagtcgtc caccagcgcg cgcaaggccg gatcccgcac ctccaccagc cccgccagcg
    31201 ccgacaccgc aggagcgaca tccgtcgcga ttgccgnnnn nnnnnnnnnn nnnnnnnnnn
    31261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31321 nnnnnnnnnn nnnnnngtcg cgattgccgt cgacgattcc gtcgccgtcg tggcgacggt
    31381 cacgcggccc tgcggcgaaa gcggtccttg tgcggaattc gcgcgctcgg cgggtgcgcc
    31441 ggaccttgcc ggaagcgcga tggcgagcga ttcggggggc gtctcgtcga gcacggtgcg
    31501 tgcggccgcg agcgcgagcg cgggatcggg cgaggtgagc gcggcgaggc gctcgcgcag
    31561 cacggcctgc accgcctcgt cccagcccgc cgccacctgc agctgcttcc acagcggcgg
    31621 cagctggtcg aggccgtggc gctcgagcca gtcgccgagc ttgccctgcg actgcacgcg
    31681 cgcctgcagc tgcatcaggg cgtcgcgacg cgcgcgcagc tcggtgagcc ggcgctgcac
    31741 cgcacgctcg tgctccagcg ccgccttcag cgcggcctgc gcgtcgggca ggcgcggctg
    31801 cgccgcggcg agttcctgct ggtgcacctc cagctcgtcc tgcagcgctt cgagccgcgc
    31861 ctcgcgctcg gcgagcacac gctcgtccgg gccgacgatc ccgccgcgct cgccctccag
    31921 gcggccgcga cgctgttgca gggcttcgag cgcacgcaag gcactcgcgc gcttggtttc
    31981 ctcgacgcgc aattgctgct cggtctgggc gagctcgcgg cgcgccgccg ccatggtcgc
    32041 gtcggcgccc tggcgcgaag agtccagctc aggcaggcgg tcggcgatct ccaggtggcg
    32101 ggcctcggcg tgctcggcgc gcagcgctgc gttctcggcg agttcctgcc agcgcgcgcg
    32161 gtcggcggcg agcgtctcgc ggcgcgagga ccagtgcccg cggtcgagtt cgagctgcgc
    32221 caggcgcgcc tccagccgcc tgcgcgcctc gccgagatgc tgcagctcgg tctccaggcg
    32281 tgcgacctcg gcgctcgccg cgaacaggtc gttctgcgcc acgtgcaccg cttcggatgc
    32341 ctcgaagtgc gcgtcgcgcc gcgactccac gctggtctcg agctcctgca ggcgggcgct
    32401 gtcggcctcg atgcggctgc tcgcctcgtt caaggaggcc gccacgcgcg cctgctcggc
    32461 gcgcgcctcg ttgcgcttga ccagccacaa gagctgctgc ttctcgacgt gcgccgcgtc
    32521 gagctcgcgg tagcgcgccg ccaccgcggc ctgcgcctcg aggtgcacga tgcgctcgcc
    32581 gagctccatg cggatgtcgt cgaggcgggc gaggttgtcg cgcgcgtcgc gcagccgacc
    32641 ctcggtctcc ttgcgccgct cgcggtactt ggtgacgccc gcggcctcct ccaggaagcc
    32701 acggatctct tccgggcgcg cctcgatgat gcgcgagatc atgccctgct cgatgatcgc
    32761 gtaagcgcgc ggacccagac cggtgcccag gaagaggtcg atcacgtcct tgcggcggac
    32821 atggacgttg ttgatgtaat aggtcgactc gcccgagcgg tcgagcacac gcttcaccga
    32881 gatctcggca tagcgcgacc actgcccggc ggccctgccc tcggcgttgt cgaagaccag
    32941 ctcgacactc gcgcgcgaca ccggcttgcg cgtggtcgag ccgttgaaga tcacgtcctg
    33001 catcgactcg ccgcgcaacg cggacgcgcg cgtctcgccc agcacccagc gcacggcgtc
    33061 gatgatgttc gacttgccgc agccattggg cccgaccact ccgacgaggt tgccgggggt
    33121 gagcacggtg gtgggatcga cgaaggtctt gaaaccggcg agtttgagct tggagagacg
    33181 cacagcgggc agtgggaaga cagcgggacg tgcaggccgg cgatccaccc cgccgcactc
    33241 cgcttttgcg gccggcatca taccaccctg ccgaagtcat cccgtgcatc agcctcgcat
    33301 cgaaacgtta cgcagcggat tcaggcatcg tcgccgcgca gcgcggtcct gcgcccggat
    33361 tcgcgccgtc gccttccgac gggcgccgcc gccatacgca ggtatactcg gcgcccgcgt
    33421 ccattgccgg ccaaggagcc cgcgtacccg tgaatcctaa cctcgcccgc ctgcagagct
    33481 atcccttcga aaagctgcgc gccctcttcg ccggtgtcga gccgcctgcg cacctcaagc
    33541 cgatccggct gtcgatcggc gagccgcagc accccacccc cggtttcatc cgccagaccc
    33601 tggccgacaa cctcggcggc ctgtcgagct atcccgccac gctcggcagc gacgcgctgc
    33661 gcggcgcgat cgcgcactgg ctcgagcgcc gctacgggct gccgaagatc gacccggcca
    33721 gccaggtgat cccggtcgcg ggcacgcgcg aggcgctctt cgccttcgcg cagtgcgtgg
    33781 tcgacggaac gaagccgggc gccaaggtgc tgtgcccgaa ccccttctac cagatctacg
    33841 agggcgcggc cctgctcgcc aacgccgagc cgatcttcat caacaacctg cccgagagcg
    33901 acttcggctc ggacttcggc agcatcgacg aggccacctg gcgcgacatc caactcgtct
    33961 acgtgtgctc gcctggcaat cccaccggcc ggctgttgac gttagacgag tggaagacgc
    34021 tgttcgagct ctcggaccgc tacggcttcg tcatcgccgc cgacgagtgc tattcggaga
    34081 tctatttcga cgacgccgac aagccgatcg ggggcctgga ggcggcctgg cgcctcgggc
    34141 gcaccgactt ccgcaacgtg gtgatgttct ccagcctgtc caagcgctcg aacgtgcccg
    34201 gcatgcgctc gggcttcgtc gcgggcgacg cgaagatcct cggggatttc ctgctctatc
    34261 gcacctacca cggctgcgcc ctcaacccgg cggtgcaggc ggcctcggtg gtggcctgga
    34321 acgacgaaga gcacgtcgag gaaaaccgcc gcctctaccg cgacaagttc gcccgcatca
    34381 cgcccatgct gcagccctgg ctgccggtgc gcctgcccga tgccggcttc tactactggg
    34441 tgcgtacgcc gctgcccgac accgagttcg cccgccgtct gcaggctgaa tataatgtga
    34501 cggtcctgcc gggcagctac ctggcccgcg aatccaatgg cgtcaacccg ggcgccggct
    34561 tcgtgcgcat cgcgctggtg gccgacaccc acgaatgtgt cgaggccgcc gggcgcatca
    34621 tcgatttctg caaaaccctc tgagagagaa ccccccaatg cacgctctgc aatcgatcat
    34681 cgacaccgcc ttcgagaacc gcgccagcct gtcgcccact tccgccccgg cagagatccg
    34741 caacgcggtc gaagaggtca tcgccggcct ggatgccggc acgctgcgcg tggccgagaa
    34801 gaaggacggc cagtgggtgg tcaaccagtg gatcaagaag gccgtgctga tctccttccg
    34861 cctgcgcgac aacgaggtgg tgcaggccgg cggcctcaac ttcttcgaca aggtggcgac
    34921 caagttcggc gactacactc ccgagcagtt ccgcgagggt ggcttccgcg tggtgccgcc
    34981 ggcggtcgcc cgcaaaggca gcttcatcgg ccggaacgtc gtgctgatgc cgtcctacgt
    35041 gaacatcggc gcctatgtgg acgaaggcac gatggtcgac acctgggcca ccgtcggttc
    35101 gtgcgcgcag atcggcaaga acgtgcacct gtcgggcggc gtgggcatcg gcggcgtgct
    35161 cgagccggtg caggccggac cggtgatcat cgaggacaac gtcttcgtgg gcgcgcgctc
    35221 cgaggtcgtg gagggcgtga tcatcgagga gaacgcggtg ctgtcgatgg gcgtgtacat
    35281 cggcatgagc accaagatct acgaccgcga gaccggcgag atcacctacg gccgcgtgcc
    35341 ggcgggcgcc gtcgtggtgc ccggcagcct gccttcggcc tgcggcaagt acagcctgta
    35401 ctgcgcggtg atcgtcaaga aggtggacgc gcagacccgc gccaagaccg gcatcaacga
    35461 gctgctgcgc ggcgcctgag cggcgcactc cggggaccgg cctgcgcgcg gttcccgggc
    35521 aaatccttgg gtgacgacag gagtccgaag gcgtgatctt cgacaatctc ttccagttga
    35581 tggccgaaaa gaaagcttcg gacatcttca tcaccgccgg caccccgatc cacatcaaga
    35641 tccagggcca caccatgccg atcaaccagc aggtcatgga cccgctgatg atccagcgca
    35701 tgctgcatga ggcgatcacc ccggcccagc aggcggtgct cgaacgcgag cgcgagctca
    35761 acctgtcctt cggtcggcgc gatctcggca acttccgcgt caacgtgttc tggcagcgca
    35821 actcgatggc cgtggtggtg cgctacatcc agagcgacat tccgagcatc ggcgcgctcg
    35881 gcctgccgcc ggtgctggac gacatcgtgc tggagaagcg cggcctggtg ctcgtggtgg
    35941 gcgcgaccgg ctcgggcaag tccaccacgc tcgcggccat gatcgaccac cgcaaccgca
    36001 accgcagcgg ccatatcctc accatcgagg atccgatcga atacctcttc aagcaccgca
    36061 aatcggtggt caaccagcgc gaggtgggca tcgacaccct gagctggggt gaggcgctgc
    36121 gcaacgcgat gcgccaggcg cccgactgca tcctgatcgg cgagatccgc gaccgcgaga
    36181 ccatgcaagc cgccatctcg tactcgcaga ccggccacct gtgcctggcg acgttgcatg
    36241 ccaacaacgc ttatcacgcg ctcaatcgca tcgtcaactt cttcccgctg gagaaccgcc
    36301 agctgctcta cctcgacctc gcggtggcgc tgcgctgcgt gatctcccag cgcctggtgc
    36361 gcaagcccga cggcaagcgc atcccggccg tcgagatcct catgaacacg cggcacatcg
    36421 ccgagctggt cgagcgcggc gagctcaatg cgatcaagga ggccatggag cagtcgctcg
    36481 cccagggctc gcagaccttc gagcaggcgc tctaccgtct ccaccacgac aacatcatca
    36541 ccctcgagga ggcgctcgcc aacgccgact cgccgagcaa cctgcactgg ctgatcaaca
    36601 acgcgcagat gcagagcgat ccgatgctga ccgtcaacgg caacagtgcc atctccggct
    36661 tcgaggcgac caacacggaa ggtgcctcct tccaggagtt caccctggac ctcaacaaca
    36721 actaggcgcc cgcgcggccg ccctccccca tgcagaccag cccctccagc tcctccaccc
    36781 tcgcgctcgc ctgcgagctc atcgcccgcc cctccgtcac gccggaggac gccggctgcc
    36841 tcgaactcgt cgcctcccgc cttgccccgc tcggcttccg cctcgaacgc atcgacagcg
    36901 gcggcgtctg caacctgtgg gcgcggcgcg gcattgcggc gcccgtgctg tgcttcgccg
    36961 gccacaccga cgtcgtgccg cccgggccgg acagcgcgtg gtcctcgccg cccttcgcac
    37021 cgacgatccg cgacggcgtg ctgtacggtc gcggtgcggc cgacatgaag tcctcgcttg
    37081 ccgccttcgt caccgccatc gaacgcttcg tcgccgccca tcccgaccac gctggcagca
    37141 tcgcgctgct gctgacgtcg gacgaggaag gcatcgccac ccatggcacg gtcaaggtgg
    37201 tcgaggcctt ggccgcgcgc ggcgagcgcc tcgactactg cgtggtgggc gagccgacct
    37261 cggtgcagac gctcggcgac atgatcaaga acggccgccg cggctcgctg tcgggtacgc
    37321 tgcgggtcaa gggccagcag ggccacgtcg cctacccgca gctcgcccgc aacccgatcc
    37381 accttttcgc gccggcgctc gccgagctcg cggcgatcga ctgggaccag ggcaacgagt
    37441 tcttcccgcc cacgacctgg caggtgtcga acatccacgc cggcaccggc gccaacaacg
    37501 tgatccccgg tgtgtgcgag gtgatgttca atttccgctt cggctcggtg tcgagcgcgg
    37561 acgagctgaa aagccgcact tgcgaggtgc tcgaccgcca cgggctggac tacgagatcg
    37621 actggcacct gtccggcaag cccttcatca ccgggcgcgg caggctggtc ggcgcgctgt
    37681 cggccgcgat ccgggagacg ctgggagtcg agaccgagct gtcgacgacc ggcggcacct
    37741 ccgacggccg cttcatcgcc gagatctgcg ccgaggtagt cgagttcggc ccggtcaatg
    37801 cgtcgatcca caagatcgac gagcacatcg caaccgatgc gatcgaaccc ttgtccgtgg
    37861 tttatgagcg caccttgacc gccctgctcg tccccggccg cctgtaggag cgacgcaagt
    37921 cgcgatgacg gccccctccg cccccaatct accgaacaga cttgccatgg ccaaccaaca
    37981 acacgccgac gacgacaacc tcgcccttga acagcacgac gaccacgagc atgaacacgg
    38041 cccgctcgcc gagctcgtga ccgtgcgcga ctggctacgc tacgccgtca cccgcttcaa
    38101 tcgcgccggc gtcttctgcg gccatggcgt gcacgacagc ttcgacgagg cggtgtggct
    38161 gatcctcggc agcctcgccc tgccgctcga ccgcctggag ccgttcatgg acgcctgcat
    38221 cccgtccgag gagcgcatcg gcctgctcga agccatcgag cagcgcgtcg acgagcgcgt
    38281 ccccaccgcc tacatcctcg gcgaggcctg gctgggcgac ttccgcttca ccgtcgatcc
    38341 gcgcgtgatc gtgccgcgct ccttcttcgc cgagttgctc gaggacggct tcgcgccctg
    38401 ggtggaggat ccggaggcgg taggcagcgt gctcgacatg tgcaccggct cgggctgcct
    38461 ggcgatcctg atggcgcacg ctttcccgaa cgcgcacgtg agcgcggtcg acctgtcgga
    38521 ggacgcgctc gatgtggcgc gcatcaacgt tgccgactac ggcctggagg atcgcatcga
    38581 gctggtgcac ggcgatgtct tcgaggggct ggaaggccgc cgcttcgacc tcatcctgag
    38641 caacccgccc tacgtgaccg cagaggccat ggaagccctg ccgccggaat acctgcacga
    38701 gccgcagatg gcgctcgcct cgggtgagga cgggctggac gtggtgcgcc gcctgctcgc
    38761 cgaggctgcc gaacacctca atcccgaagg catcctggcg gtcgaggtcg ggcataatcg
    38821 cgccatcgtc gaagccgcct tccccgacct gcccttcgag tggctgccta cccgcggcgg
    38881 cgacgacatg gtgttcgtcc tgcggcgcaa ggacctgtaa tcccgccgcc tcggccgccc
    38941 ctaccccccc tcacctcatt caggaacatg gcaagcgaaa gaccgatcct gctggtcgag
    39001 gacaacccgg acgacgaggc gctgacgctc cacgccctgt cgcgcaaccg catcaccagc
    39061 ccggtggtgg tggcgcacga cggcgtggag gcggtggagt acctgttctc gaccgggagt
    39121 cgcgcgggac agccgctgcc gccgctgccg gcggtcgtgc tgctcgacct caagctgccg
    39181 cgcatcgacg gcctggaggt gctgcgccgc atccgcgccg accagcgcac ggcgctgctg
    39241 ccggtggtgg tgctgaccac ctcgcgggag ccgctcgatc tcgcccaggc ctaccgcctc
    39301 ggagcgaaca gctacatccg caagccggtg gatttcgacc gcttcgagcg tgcgatcggc
    39361 ctgatcgggc actattggct ggaactcaac gagtccaccg aaacggtcgc gcgcggggcg
    39421 tattgagcga cgcgggagtc ccccgtaggc gcgacgccag tcgcgattcc agccgaaccg
    39481 cgtcgacgac gacgcatgcc gcgtccggcg ctcgcgcacc ggacgcgcgc accttcaggc
    39541 gacctcgtcg atccaggcct gctggatgcc ttcgaggatg cgttcgccgc agcgcttggg
    39601 atcgtcgtcg aaatcgggca gcgccatcgt ccagttcatc aggtcgacga aattcacccg
    39661 ggtcgggtcg acgtcggggt gggcgtcggc gagctggatg gcgatctcct gaacctcggt
    39721 ccacttcatg tcagtgcttt ccttcgcgag ccatgttgat ggtgtagcgc gggatctcga
    39781 ccaccagcgg ggtcgcggcg accaccgcct ggcaggacag gcgcgactgc ggttccaggc
    39841 cccaggcctt gtcgagcagg tcttcctcct cgtcctcggc ggcgttcagc gaggagaacc
    39901 cctcacgcac gatgacgtgg caggtggtgc aggcacagga cttctcgcag gcgtgttcga
    39961 tgtcgatgcc gtgttcgagc agcgcgtcgc agagggtggt gccgggggcg gcctcgagga
    40021 cgctgccgtc cgggcagagt tcgacgtggg gcaggacgat gatctgggtc atgggcgaag
    40081 tatcagatgg ggagttcgtc gaccttgtgg ccggccagcg cgctgcgaat gctgttgtcc
    40141 atgcggcgcg cggcgaaggt gtcggtggcg cgcgcgagcg cgtcgatgcc ggccttgatg
    40201 gcacggttgt cggtgccggc gcgcagctcg cgcaggcgcg cgatcacgct gcggatttcg
    40261 gcggactcga cggcggagag caggtggccg tcgtggccga gcgcctgctc ggtggcttcg
    40321 acgacacggt cggcctcgac ctgctgctcg cgcagcgcgc gcgcggccat gtcgtcgccg
    40381 gcgtgatcca cgcccgagcg caacatctcg gcgatctcgt cgtccgacaa gccgtacgag
    40441 ggcttgacca gcacgctggc ctcgacgccc gaggacatct cgcgcgccga caccgccagc
    40501 aggccgtcgg catcgacctg gaaggtcacg cggatgcgcg ccgcgcccgc caccatgggg
    40561 ggaatgccgc gcagctcgaa gcgcgcgagc gagcggcagt cggagacgag ctcgcgctcg
    40621 ccctgcacga catggaaggc catcgcggtc tggccgtcct tgaaggtggt gaattcctgc
    40681 gcgcgcgcga tcggcagcgt ggcgttgcgc ggcacgacct tctcgaccag gccgcccatg
    40741 gtctcgaggc cgagcgagag cgggatgacg tcgagcagca gccagtcgtc ctggtcggcg
    40801 cggttgcctg cgagcacgtt tgcctgcatc gccgcgccca gtgcgacgac cttgtcgggg
    40861 tcgaggttgg tcaggggctc ctggccgaag tagttcgcga ccgcgcgctg gacgtggggc
    40921 atgcgggtcg cgccccccac catcaccaca cccttgatct cgtcgggcgc gaggccggcg
    40981 tcgcgcaagg ccttgcgcac cggggccagg gtcttctgga ccagatgcgt ggtcatctcg
    41041 gcaaactcct cgcgggtcac cacgaggtcg acctcttcgc ccgaggccag gcgcacatgc
    41101 accggggcct cgtcacagct cgtcaacagc tccttggcct cgcgcgcctt catctgcagg
    41161 cggcgcgcgt cttccagcga gggcggcgag atcttggcgc ggtcgaggat ccagcagaac
    41221 aggcgatgat cgaagtcgtc gccgccgagc gcggcgtcgc cgttggtcga cagcacctcg
    41281 aacacgccgc gcgagagctt gaggatggag aggtcgaagg tgcccccgcc caggtcgtac
    41341 accgcgtaga cgccctcggc cgcgttgtcg aggccgtagg ccaccgcggc ggcggtcggt
    41401 tcgttgagca ggcgcagcac gtcgagcccg gccaggcgcg ccgcgtcctt ggtggcctgg
    41461 cgctgggcgt cgtcgaagta ggccggcacg gtgatcaccg cgccgaccag cgcgccgccg
    41521 aggctggcct cggcgcgctc gcgcaggcgg cgcaggatct ctgcggagac ctccaccggg
    41581 ctcttgacgc cctgcaccgt gcgcagccgc accatcccgg ggctgtcctc gaagtcgtag
    41641 ggcatcgcct cgatgtgggt gacgtccttg aggccgcggc ccatgaagcg cttgaccgag
    41701 acgatggtgt tgcgcggatc gaccgcctgc gccttgagcg cacccagacc gacctcgatg
    41761 ccgccatcgg cgtgatagcg caccgccgag ggcagcatgg tgcggccggc ctcgtcgggc
    41821 aggcagatcg cgatgccgtt gcgcacggtg gcgacgagcg aattggtggt cccgaggtcg
    41881 atgccgactg cgagccggtg ctggtgcggc tcggtggaca tgccgggttc agtgatttgc
    41941 aacagagcca tcggtgctgt tccgtgcctt ttcgagttct agttttcgag cgcgagcagc
    42001 gcttcgtcga tttcctgctg gagcttctcg atgaacatca gccgacgcac ggtgtcggcc
    42061 gcggcctcgt agtcctggtg ctcgtcgagc tgcgcggcga gatgcccgcg cacctcgcgc
    42121 gcatgcccga gcaggcgcct gtgcagatct tcgagctcgg agacctcggc cgcctcgcgc
    42181 gcctcctcca ccgcctcgcg ccactccatc tgctccatca ggaactcggg ggacatcgcg
    42241 gtgttggtct cgagcgcggc gtcgacgccg gcgagctcga gcaggtactg ggcgcgcgcg
    42301 agcggcttgc gcagcacgcg aaagccttca ttgacccgcg tcgcccactg catcgagcgc
    42361 cgcttctcga agtcggacag gtgcgcgtgg cggtcgggat gcacgcgccc ctgcagctcg
    42421 tgccaggcgg cttcgagcgc gccttcgtcg agcgcatagc ggcgcgggag gccgaagagg
    42481 gcgaagtagt cctgcgtcag gtcgatgctc atcgggcccg gctcagacgt tgaagctctc
    42541 gccgcacccg cactggtcct tgacgttcgg gttgttgaac ttgaagccct cgttgaggcc
    42601 ctccttgacg aagtcgagct cggtgccgtc gagataggcc aggctcttgg ggtcgatgac
    42661 gaccttgacg ccgtggctct cgaagacctc gtcctcgtct tccgtctggt cgacgaactc
    42721 gagcttgtag gccatgcccg aacagcccga ggtcttcacg cccagacgga tgccgaagcc
    42781 cttgccgcgc ttggcgatga agttcgagac gtggcgcgca gccgattcag atagcgtgac
    42841 agccatgttt cctcctcttc gacgaaggcc gctcaggcct catgcttctt tttatagtcc
    42901 gccacggcgg ccttgatcgc atcttcggcg aggatcgagc agtggatctt gaccggcggc
    42961 agcgcaagct cttcggcgat ctgggtgttc ttgatgtcga gcgcctggtc gagggtcttg
    43021 cccttgaccc actcggtgac cagcgagctc gaggcgatcg ccgagccgca gccgtaggtc
    43081 ttgaacttcg cgtcctcgat cacgccgtcc ttgccgacct tgatctgcag cttcatgacg
    43141 tcgccacagg cgggcgcgcc gaccatgccg gtggccacgc cttcttcttc cttgccgaag
    43201 gagccgacgt tgcgggggtt ttcgtagtgg tcgaggacct tttcgctgta tgccatgttt
    43261 tttctcctgc tggggcggtg aatcgcttca cctgcaattt atatcatctt ctgcactaaa
    43321 gttcagtggg ctgcccactg caccgcgtcg aggtcgacgc cgtccttgta catctcccac
    43381 agcggcgaga gctcgcgcag cttgccgatc ttgtcgtgca ggagcttgat cgtgtagtcg
    43441 atctcttcct cggtggtgaa gcggccgatg gtgaagcgga tcgagctgtg cgcgagctcg
    43501 tcgttgcggc cgagcgcgcg cagcacgtag gacggttcca ggctggccga ggtacaggcc
    43561 gagccggagg acaccgcgat gtccttgacc gccatgatca gcgactcgcc ttcgacgtag
    43621 gcgaaggaaa tgttgaggtt gtgcggcacg cgctgcgcga gatcgccgtt cacgaaggtg
    43681 gcctcgaggt cggtgaggcc ggcgagcagg cggtcgcgca gcttgccgac gcgggcgttc
    43741 tcctcggcca tctcctcgcg cgcgatgcgg aaggactcgc ccatgccgac gatctggtgg
    43801 gtggcgagcg tgcccgagcg caggccgcgc tcgtggccgc cgccgtgcat ctgcgcttcc
    43861 aggcggacgc gcggcttgcg gcgcacgtac agtgcgccga tgcccttcgg gccgtaggtc
    43921 ttgtgcgcac agaagctcat caggtcgacc ttgagcctgt cgaggtcgat atcgaccttg
    43981 ccggtggcct gcgccgcatc gacgtggaac acgatgccct tctcacggca gatctcgccg
    44041 atttcggcga tcggctgcac cacgccgact tcgttgttca cgaacatcac cgagaccacg
    44101 atggtgtcgg gacggatcgc cgccttcaac acctcgaggt cgatcagccc gttctcctgc
    44161 acgtcgagat aggtggcctc gaaaccctcg cgctcgagct cgcgcacggt gtcgagcacc
    44221 gccttgtgct cggtcttcac ggtgatcagg tgcttgccct tgccctggta gaagtgcgcc
    44281 gcccccttga tggcgaggtt gttcgattcg gtcgcgcccg aggtccagat gatctccttg
    44341 gcgtcggcat tgaccagtgc ggcgacctgt tcgcgcgcat cctcgaccgc cttctcggcc
    44401 tcccagccgt aggcgtgcga gcggctcgcc gggttgccga aatgctcggt cagccagggg
    44461 atcatctttg ccgccacgcg cggatcgacc ggagtcgtcg ccgagtagtc gaggtagatg
    44521 gggaacttca gcattgtgtc tctccgcact ttcttcgtta gcgagttcgg tgaatggcca
    44581 gcgtggccgg ttcaggcccc gatcgaggcc aggttcttga gttcgtccgt cacccggacg
    44641 aaggtttcga tgaagctgcg cacgtcgtcc gcgcgcgtgt cgcgaccgag gctcacacgc
    44701 accgcggcgc gcgccagccc gcgctccacg cccatcgcca gcagggtgcg ggacggttcg
    44761 gggttcgcgc tcgagcacgc cgagccgctc gcgcacgcga agcctgcacg gtcgaggcgg
    44821 ccgaccaggg tctcgccgtc gatgtcctcg accgcgaaga atacggtatt cggcaggcgc
    44881 ggggcagccg ccgagaagat ccgcgcgccg cgcccggcga gcgcggcaca gagttcgtcg
    44941 cgcagggcgg ccaggcgcac gccctcgcct tcgcgccgcg cgaccgcgcg ctcgcaagcc
    45001 acgccgaagc cgacgatcgc ggccacgttc tcggtccccg agcgcaaccc gcgctcctgg
    45061 ccgccgccgg cgatcagcgg cgcgagctcg acgcgcttgt ccagcaccag cgcacccgcg
    45121 ccgagcggac cgccgatctt gtgcgcggac agcgtcatcg catgcacgcc gagcgcgcgg
    45181 aaatccaccg ccaccttgcc cagcgcctgc accgcgtcgg tgtggaacca ggcgccggcg
    45241 gcgcgcgccg cgcgcgccag tgcggcgacg tcctgcagca cgccggtctc gttgttggcc
    45301 agcatcaccg acaccaggcc cgggcgggct tccagcaccg cgctccagtc ggcggggtcg
    45361 atcacgccct gcgcatcgac cgcgatctcg cgcagcgtcc agcccgcgcg ccgcagctgg
    45421 cgggccggct cgcgcacgca ggggtgctcg atcgcactca ccgccaccac gcccggctcg
    45481 cggttgggcg cggcgccctt gaggaagaga ttgttggctt ccgagccgcc gctggtgaag
    45541 atcacctcgg tcgcgtgcgc gccgaccgcc gcggccaccc gcgcgcgcgc ctcgtccacc
    45601 gcggcacgcg cctggcggcc gtactcgtgg cggctggagg cgttgccgaa gcgcgccggc
    45661 tcggcggcgc cgagccaggg cagcatggcc tcgcgcaccg ccggatcgag cggcgtggtc
    45721 gcgttccagt cgaggtagat gggagcgaag ttcatgcgcg acctcagacc gcggcgatct
    45781 cgtgcaccgg catgcggctg cgccgccgca cgctcttgag caccgtcatg tcggcgtcgg
    45841 gccgtacctg gtgcttgacc agcgcctcca ggctcaccga gtcgaggtag gcgtagatgc
    45901 gctggttgag gttggaccac aggtggtggg tcatgcacac gtgatcgtcg tggcagtttt
    45961 ccttgccgcc gcactgggtg gcgtccatcg gctcgtcgac cgcgatgatg atgtcggcca
    46021 cggtgatctc gtccatcgcc cgcgccagtc ggtagccgcc gccgggaccg cgcacgctgt
    46081 tgaccagctt gtgcctgcgc agcttgccga agagctgctc gaggtaggac agcgagatgc
    46141 tctggcgctc ggcgatgccc gccagcgtga cgggcccgtc cccctggcgg gcagccaggt
    46201 cgatcatcgc ggtcaccgca aaccgtccct tggtcgtaag tcgcatgagg ctctcctcga
    46261 aggctgtccg ggctcgcgcg tgccgggggc cgccgaatat cccatcattt ttgtcgagta
    46321 tatggatccc gacaaaaacg gtcaactaat cgggagacga gtcttgcgct gcaaggcgcg
    46381 aggggcgccg ttgcagccgc ggcgccagcc gcgaacacgg cgctcgatcg tccggcaccg
    46441 caggcgtgcg aatcaatcca ccatccgcga cagccggccc gggtcgaaat ggtcgctcgg
    46501 ggcgaccgtg tcgtcgagct tgatccccgc ggcctcgaga cgggccagga tggcctgcag
    46561 gcggcggtcg gtctcaacgg aatggtcgag caggccgtgc agcgccttgg cgaccgggtc
    46621 gtccatgtcg cgggtcacgc cgtaggcgct gaagccgaac tgctcggcct tctgctcgcg
    46681 cgcggcgtcg cgctcgctat ccttgtccga gtcgagaatg cgagccggat tgccgacggc
    46741 ggtcgcgccg gcgggcacgg gcttcaccac caccgcgttg gagccgacct tggcgccgtc
    46801 acctaccgtg aagccgccga gcaccttggc gcccgccccc accaccacgc cacgccccag
    46861 ggtgggatgg cgcttggtgc cgcggtagag ggaggtcccg ccgagggtga cgccctggta
    46921 gatcgtgcag tcgtcgccga tctccgcggt ttcgccgatc acgacgccca tgccgtggtc
    46981 gatgaacacg cggcggccga tcttcgctcc cgggtggatc tcgatcccgg tgagcagccg
    47041 gccgacgtga ctcgcgaagc gccccgccca gaaccagccg cggcgccagg cgccgtgagc
    47101 gaggcggtgg aacatcagcg cgtggatgcc gggataacag gtcagcacct cgagcgtgga
    47161 gcgcgcggcg gggtcgcgct cgcggacgct ggccagatct tctcggaggc ggctgaacat
    47221 gatggaagga ctcccggatg tgcggtggaa aaaacacggc ggaccgcgtc gagcgggcgc
    47281 cacgcattca attcccgaga attttaatcg actatagcgc cccgccgaaa gcccgcgctg
    47341 cgaccgctcc acccgcaaga cgcggcttgc acgcgtggcg cgggcgggca atgatcgcgc
    47401 gggatcactc cggacctgcg ctcatgctcc agctcttccg ccgcctgctc ggcatcccgc
    47461 ccgcccgccc cctggtcgac gagtcgcagt ggcggcgggt cgaagcgagg ctgcccttcc
    47521 tcgaccacct cgttcccgac gatcggttgc gtctgcgcga gatggcgctc gccttcctcg
    47581 ggtgcaagga gttccatggt gcgcaggggc tggaactcga cgacgacatg ctgctggcga
    47641 tcgcgctgca ggcctgcctg ccggtacttc acatcggcct gcaggcttac gagggctggg
    47701 tgggagtgat cgtctatccg ggcgacttcc tgatcccgcg ccgcatcgtc gacgaggccg
    47761 gcgtgctgca cgaatacgag gacgaggtgc tgggcgaggc ctgggccggc gggccggtgc
    47821 tgctgtcctg gtttcccggg aacgacgctc cggcgggcgt gaacgtggtc attcacgagt
    47881 tcgcccacaa gctcgacatg gagaacggcg acgtggacgg cctgccccgc ctgcgccccg
    47941 gcatgtctcg ccgggactgg gccaaggcct tcggcgaggc ctacgaggcg ttctgcgccg
    48001 aggtcgaccg cggcgtcgac accacgatcg acccttatgg cgccgagcac ccgggcgagt
    48061 tcttcgcggt ggtctccgag gtcttcttcg aggatccgct cgtgctgcac aggcgctttc
    48121 ccgcggtcta cgatcaactc gtgcacttct acggaatgga ccccgccgcc cgcgcggcgg
    48181 ccgctgccgg aggctgacat gagcgcgccc aaacgcctgc tgatcgtcca tcacacccag
    48241 agcggcaaca cgggccggct cgccgccgcg gtgctcgagg gggcgctgcg cgtgagcgag
    48301 accgagacgc ggttgctgcg cgccttcgac gccggcaccg ccgacctgct cgcgtgcgac
    48361 ggcctgctgc tcggtacgcc ggaaaacttc ggctacatgt cgggcgcgct caaggacttc
    48421 ttcgatcgca cctactaccc ctgcgagggc aaactcgtcg gcctgcccta tgcggtcttc
    48481 gtcagcgccg gcaacgacgg caccggcgca gtgcgcgaga tcggccgcat cgccaacggc
    48541 tacggctgga agacggtcgc cgaggccctg atcgtgcggc gcgagatcac ccccgccgac
    48601 ctggagcgcg cgcgcgagct cggcgaggcg atggcggccg ggttggcgat gggggtgttc
    48661 tgagcgtgcg aggggcgagg cgtcgggcgt gcgatgccgc ggggacgcgg tcgcggcgag
    48721 cacgtcgggt aagttcacgt ttgcgtcaat cgggatcggt tttcacgaag cggtaacgat
    48781 ggtcgtggcg gagtcggttc gcgtgaccta agcttcgatc gtgcccactg cgcagggcac
    48841 ccggtcttta atcgaacgga ggattgctta tggccatcac ctggattctg gttgccaacg
    48901 ccagcctggc gaagctgtac gccaacctgg gcccgaacaa ggggctcacg ctggtcaagg
    48961 aactgatcca cccggaaagc agacaaaaga atggcgactt ggtctcggac cgctcgggcg
    49021 cgaatggctc gaacggcacc ggtggcggct cgatgcagcc gcagaccctg cccaagcagc
    49081 acgaggccaa agtgttcgca caggagattg cccagacgct ctaccatgga cgcacctcga
    49141 atgcattcaa gcgtgcgatc ctggtcgctc cacccgcctt catgggcctg ctcaacgcgg
    49201 tcatcgacgg ccccaccgcg cagatggtca cagaccgctt cgaaaaggat tacaccaagt
    49261 cgacgcagga cgagctgggg gaacgcctcg gctcgacgat ctttctctga gggaggacgt
    49321 tcacgaaggc tccagatccg atcagccaat ggccgagggc acctgcccga gccaaggagg
    49381 atgtgcttga ccgaaccccc gaggatgcag ctttcctgac ggatgcagta cacacgatga
    49441 agacatcgtc tccactacgg agaccccggg cacgcgagac gtgacccgat cgaggacggg
    49501 gcgcattgcg ccccgttcgc tttcggctcc gcccgatcga atcacccacg ccgcttcaac
    49561 ggccagcgcc cgatcgtgcg gtagtgcgca ccggcggcgc tgcgctcgga ggcgaccagg
    49621 acgaagctgc cgaccgtcca tcgcagaggt gcgagctgcg cctcgcgggg cgcagcgtgg
    49681 gcgttgcgca cgagggtgac atgcggggag aaggcacgcg cctcgagtgc gcagccgtcc
    49741 gcgcgcagcc gtccttccag atcctgcgcg agcgcggcca gcgctggcgg caccatcgac
    49801 ggaccggtcc acaggatccg gttgcgatgc cagctgccga cgcgatcgag gtcgagcgcg
    49861 aagccctccc cctggacgcc cgcggccacc gcgcgcaatc gctccacctg ccgcacaggc
    49921 gtttcgccga ggaaggccag ggtcaggtgg atggtgtcac gacgcatcgc gcgaccgccg
    49981 cactcggaat gcagcgcccg tgcacgcgca tgcagcccgg cgcgggtggc ggcatccggc
    50041 cacagcgcga agaagagccg cagattcggc gccctccccg cgtccgctgc agcttcagcc
    50101 atgctccgcg cgcgcgatca gcgcgaccac ctgcgcggcg atgccttcgc cacgtccggt
    50161 gaagccgagc ttctcggtgg tcttgccctt gatgttgatc gccgtcgcgt ccatctccag
    50221 gtcggcggcc aggttggcca ccatcgccgg ggcgtgcggc aggatgcgcg gcgccttgca
    50281 gatcagcgtg gcatcgaggt tcacgacctc ccagcccgct gcgcgcacgc gcgcgaaggc
    50341 ctcgcgcagc agcacgcggc tgtctgcgcc ggcgtgggcc gggtcggtat ccgggaagag
    50401 ccggccgata tcgcccaggc cggcggcgcc gagcagcgcg tccgtcaccg catgcaacag
    50461 cacgtcggca tccgagtggc cgagcagtcc atgcccgaag ggaatgtcga cgccgccgac
    50521 gatcagcggc cggccgggga ccagggcgtg cacgtcgaag ccctggccga tgcgaaaggg
    50581 gatgctcatg cgttgtggcc ctcacggttc tcgaggatcc actcggccag gtgcaggtcg
    50641 agcggatagg tgaccttcag gttggtggcg tcgccctgca ccaggcgcgg acgcaggccg
    50701 gcggcctcga tcgcgctcgc ctcgtcggtg acctcgcgcg cgccttcgag cgcgcgtcgc
    50761 agcatggcgt agcggaacat ctgcggcgtc tgcgcctgcc acaggctgtc gcgcggcacg
    50821 gtctcgcaca catggcggtg ctcgtcggca cgcttgaggg tgtcggccac cgggactgcc
    50881 agcaagccgc cgacctcgtc gtgggcgagc tcgcgtacga gcttgtcgac atgccagggc
    50941 gccaggcagg ggcgcgcggc gtcgtgcacc agcacccagt ccccagccgc cgcctcgccg
    51001 gcgatcgcgc gcaggccgcc gagcacgctg tcggcgcgcg tggcgccacc gcagaagagc
    51061 gggcgcagct tggggccgag cgcgctccag tcgtgcgttg cccactccgt gtcgtccacc
    51121 gagagcacga cgaacaccgc gtcgatcgca ggcgcggcgc acagcaccga aagcgcatga
    51181 tggatcaggg ggcgaccgag cagcgacaga tactgcttgg gacgcgcggc ggccatgcgc
    51241 gagccgctgc ctgcggcggg cacgatggcg aagtggcgtg ggtggctgat ctgcatggcg
    51301 cgcattctag ccgcgccgcc cgccgcgcgg gcttgtgcaa gcacgggttt cgatgcggga
    51361 cgatcccggc cgggctgcct ataatggaat ctgttccaac gatgacatcc ccgcaggatt
    51421 cctccatggc actcgcttcg atccgcccgg cagccgtggc cgggcagttt tatcctcgcg
    51481 acgagcgcgt gctgcgcacc cagctcgccg agatgctctc caccgcggtc gccttcgagt
    51541 cggcgcccgc gcccaaggcg atcatcgtgc cgcacgcggg ctacatgtac tccgggccgg
    51601 tggcggcgag cgcctatgcg ctgctcgcac cgctgcgcga gcgcatccgc cgcgtcgtca
    51661 tgctcggccc cacgcatcgc gtggcagtgc gcggcttctc gctgccggca gcgcagcgct
    51721 tcgccacccc gctcggcgag gtcccggtcg cacgcggcga ctgggaggcc ctgcaggcgc
    51781 gcgacgatgt cctggtcgac gatcgcccgc acgcgctcga gcactgcctc gaggtgcagt
    51841 tgcccttcct gcaggtctgt ctcgagcgct tcgagatcgt cccggtgctg gtcggcgacg
    51901 cctcgcccga agccaccgcc agcctgatcg agcgcctgtg gggcggcccc gagaccctga
    51961 tcctgatcag ctcggacctc tcgcattacc tgagctaccg cgaggcgcaa tggtccgacc
    52021 gcgcagcggt ggaccaggtg ctcgaactgc gccccggtct cgaccacgag caggcctgcg
    52081 gcgccacccc gatcaacggg ctgctgctcg ccgcccgcaa gcacgacctg caggcccacc
    52141 tgctggacct gcgcaactcc ggcgacaccg cgggcgaccg ctcgcgcgtc gtcggctaca
    52201 ccagcatcgc cttttgtgaa gcggaacccc caagccatgc ccgacactga tttcggtacc
    52261 accctgctcg cgctcgcgcg caccgcgatc gcccaccgcc tcggccgcgg ccccgccccg
    52321 gtcatcccgg acgatccccg cctgcacgag aagggcgcga ccttcatcac cctcgccaag
    52381 gacggcgagc tgcgcggctg catcggcagt ctgcggcgct cgcgcgagct cggcgaggac
    52441 gtgatccaca acgcgctcgc ggcggccttc gaggactcgc gcttcgagcc gctcgcgccc
    52501 gaggagctcg gcgcggtggc gatcgaggtc tccatcctgt ccgaaccgga gttcctcgac
    52561 ttcttcggcg aggaagagct gctcgcccag ctgcgccccg gcgaggacgg gctgatcatc
    52621 ttctccggct gccacagcgc caccttcctg ccccaggtgt gggagcagct gcccgagccg
    52681 cgccagttcc tcgccgcgct caagcacaag gccggtctgc ccaccaaccg cgaggtcgtc
    52741 gagctgatgg ccgcgcgcta tcacgtggaa aagttcaagg agaccgaacg ggagccctca
    52801 tgaacacgaa gcccgcgcca gccgccgagg tcggcagccg ccatcccgcg cgctactggc
    52861 accgcatcga cgacggtcgc taccagtgcg acctgtgtcc gcgcctgtgc aagctgcacc
    52921 ccggccagcg cggggcctgc ttcgtgcgca tgcgcgaggg cgacgacatg ctgctcacca
    52981 cctatggacg cagctcgggc ttctgcatcg acccgatcga gaagaagccg ctcgcccact
    53041 tctacccggg cagcaaggtg ttctccttcg gcaccgccgg ctgcaacctg gcgtgcaagt
    53101 tctgccagaa ctgggacatc tccaagtcgc gcgacctcga caccctgatg gacgaggcct
    53161 cgcccgcgga aatcgccgag accgcgctgc gctggggctg ccacagcgtc gccttcacct
    53221 acaacgaccc ggtgatcttc gccgagtacg cgatggacgt ggccgacgcc tgccatgcgc
    53281 gcgggctgaa gaccgtggcg gtgaccgccg gctacatctc cgagtgcgcg cggcgcgatt
    53341 tcttctcgcg catggatgcg gccaacgtcg acctcaaggg cttcaccgac gacttctacg
    53401 tcaagctgtg cggcgcccat ctgcagcccg tgctcgacac cctggtatgg ctgcgccacg
    53461 agaccgacgt gtgggtcgaa ctcaccaccc tgctgatccc cggccacaac gactccgaca
    53521 gcgaggtcac cgcgctggcg aactgggtgc gcaaggagct gggcgccgag gtgccgctgc
    53581 acttcagcgc cttccacccc gacttcaaga tggacgagct gccgcccacg ccgcccgcca
    53641 cgctcgcgcg cgcgcgccgc atcgccctcg acgccggcct gaagcacgtc tacaccggca
    53701 acgtgcacga catcgaaggc gacacgaccc gttgcgcgag ctgcggcgag cccctgatcg
    53761 tgcgcgactg gtacgagatc cgccgctacg ccctcggcaa cgatggccac tgtccgcact
    53821 gcggcagcgt gctcgccggt cgcttcgctg cagtgggcgc cgcggtcggc gacgccttcg
    53881 gcgcgcgccg catcccggtc gccatccacc gtcacggcac ctgagcgcat tcgaccccca
    53941 acgtgaaact tcgaaacacc ctcatcagca tccccgcaca gggcgcgtgg ctcgacggcc
    54001 tgcttgcaca cgcgccggac gtgcgcgggc tcgccatcct cccgcagtcg agcggacacc
    54061 ctgcgctcga cagcgggccc cggccggtcg atgtcgccct gcagtcgcgc ggcttcgcca
    54121 ctctccgggt cgacctgctg acccggcagg aggcgctgcg cgaccccgat gcgtcgtaca
    54181 acatcgcccg cctgaccgag cgcatcctcg cggcggtcga gtggatcgcc caccagccgc
    54241 cgctcgccgg gctgcagctc ggcctcgtca cgcaggacac cggctgcgcc gccgcgatcc
    54301 gcgcggcggt caaggcgccg gagaccttcg cggcgatcgc ctgtgtcgac ggccgcgccg
    54361 acctcgcggg tgccgagccc ttgcgccagc tgcgctgccc ggtgcgcttc atcgtcagcc
    54421 ccggcgcccc ggagagcgcg atcctggaga aagccttttc cctgctcggg ggggcgcgcc
    54481 actggctcgc cctgcccgac accggtgacg cccgccagca ggagatcctg caggcgcggg
    54541 cggtgacgag ctggctcgaa cagcacctcg ccgcggccga gcgcacgcgg gtggaatagg
    54601 atcggagcgc gcccgcttca tgtccgcaat gtcgatttcc tcgctcggtg gcggtgacgc
    54661 ggcgtggcgc cagggcacgg cgatgaatgc cgcccgccct gccggcgcct ccggcgggcc
    54721 ggcgacggcg ggggccggcg gcaaggcgca agaggcagga gcgggggcgg ccgacacgct
    54781 ctccgaggcc gaacagcgcc aggtcgccga gctcgcggaa cgcgaccgcg aggtccgtgc
    54841 ccacgagatg gcccatgtcg ccgcgggcgc cggcctggtg acccggggcg ccagctacac
    54901 ctatcagacc ggcccggacg gccagcgcta cgcgatcggc ggcgaggtcg ggatcgatac
    54961 ctcgccgggc cgcagccccg aggagtccct ctccaaggcc gagcgcatcc gcgccgcagc
    55021 cctcgccccc gccgagccgt cgggccagga ccgccaggtc gccgcgcagg cttcccgcat
    55081 ggcaagcgat gcgcgcatgg agatcgcccg ccgcgaattc gagcaaggcg gcgcgggcac
    55141 cgaggcggcg gccacgcgcg atggccccgg ctatcgcggc atgcgagcgg cgcaggccta
    55201 ttcggcagcg ctcgctccag gccaggggcc caccctttca gccactgcct gagccttccg
    55261 ctccgcgttc gagcgatcac gccgtgcgct ggcgggccgg gatgaacgat aattctctct
    55321 ttgccggccc cgcgtcccta ccgccatgct gcccgcctcc gccacggtga cggagggtcg
    55381 cagtccgcag acgacgctcg agaaaccacg atgtccctct ccctcgacgc cctcctctcc
    55441 cggctcgcca cggtcaagct gcccaagccc ggcggccggc tcgaactcga gccgctcgcc
    55501 ggctcggcag acgccctcgc catcgcccag ctcgccagcc gcggccgcat gctggtggtg
    55561 atcacggcca acccgctcga cgcccagcgc ctgcaggacg agatcgcctg ggtcgcaccc
    55621 ggcctgcgcg cgcacctgct gcccgactgg gagaccctgc cctacgacag cttctcgccc
    55681 caccaggacc tgatctccga gcggctgtcg acgctctacg ccatcagccg cggcgagacc
    55741 gacatcgtgc tggtgcccgc ctccaccgcg ctgtaccgca tggcgccgcc gggttttctt
    55801 gccgcgtaca cctttttcct caaacagggc gagaagctcg acgtcgagca gttcaaggcg
    55861 cagatggcgc tcgcaggcta tgcgcacgtc acccaggtgg tgagcccggg cgagttctcg
    55921 gtgcgcggcg ggctggtgga cctgttcccg atgggttcgc cgctgccctt ccgtatcgac
    55981 ctcttcgacg acgaggtcga gagcatcaag accttcgacc ccgacaccca gcgcacggtg
    56041 tatcccacca aggagatccg tctgctgccg gcgcgcgagt tcccgctcga cgacaagggg
    56101 cgcacgcgct ttcgcagccg cttccgcgag accttcgagg gcgacccgac gcgcgcgtcg
    56161 atctacaagg acgtctccaa cggcatcgcc cccgccggca tcgagtacta cctgccgctg
    56221 ttcttcgacg agaccgcgac gctgctggac tacctgcccg ccgagacgcc ggtgctgctg
    56281 caccgcgacg tgccggcggc gatcgccgag ttctggaagg acacgcgctc gcgccacgac
    56341 ctgttgaagg gcgaccgcag ccgcccggtg ctggcacccg agcagctctt cctgaccgac
    56401 gaggccttct tcatcgcgct gaagaacctg ccgcgcctgg cgatcggggc cgaagtgggc
    56461 gccaaagcgg gcgccgagcc cgcggccgcc cgcaccgacg cgctcgccct gcccccgccc
    56521 gaggtcgcgg tcgagcgcaa ggccaccgac ccgctgcaca agctcaaggc cttcctcgac
    56581 ggcttcgacg gtcgcgtgct gctgctctcg gactcgcccg gccggcgcga gaccatggcc
    56641 gagttcttcg ccgagtacga cgtgcgcccc gaggccacgg ccgatttcgg cgccttcctg
    56701 gattccgacg cccgcctcgc gctcggcgtc gccccgctcg cgcgcggctt cgttctaccg
    56761 gctgcaaggc tcgcggtgct gaccgagacc gagctctacg ccgccaccgc gcgcagccgc
    56821 gtgcgccgcg acgcgcgcaa ggccgccacc atggaaggct ggctgcgcga cctttccgag
    56881 ctcaagatcg gcgacccggt ggtgcacgtc tcgcacggca tcggccgcta cctcggtctc
    56941 ctccacatga acctcggcga gggcgacacc gagttcctgc atctggagta caacggcggc
    57001 gacaagctct atgtgccggt gtcgcagctg cacgtgatca cccgctacgc cggcgccgac
    57061 cccgaggcgg tcgacctgca ccgcctcggc tccggccagt gggagaaggc caagaagaag
    57121 gccgcgatgc aggtgcgcga caccgccgcc gaactgctcg cgctctatgc ccagcgcgct
    57181 gcccgccccg gccaccgctt cgacttcaag cagcatgacc tcgaagcctt cgccgaggcc
    57241 ttcggcttcg agaccacgcc cgaccagcag gccgccatcg acgcggtggt ggccgacatg
    57301 aaatccggcc gcccgatgga ccgcctggtg tgcggcgacg tcggcttcgg caagaccgag
    57361 gtcgcgctgc gcgccgcctt catcgcggtg gccgacggca agcaggtggt ggtgctgtgc
    57421 ccgaccaccc tgctcgccga gcagcactac cagaccttcg ccgaccgctt cgccgactgg
    57481 ccgatcaagc tcgccgagct gtcgcgcttc aagtcggcca aggagcaggc cgaggccctg
    57541 cagctcctcg cggagggcaa ggtcgacatc atcatcggca cccaccgttt gctgcagaag
    57601 gacgtgatct tcaagcgcct cggcctggtg atcatcgacg aggagcaccg cttcggcgtg
    57661 cgccagaagg aggccttgaa gcaattgcgc tccgaggtcg acatcctgac cctgaccgcc
    57721 acgccgatcc cgcgcaccct gggcctggcg atggagggcc tgcgcgagtt ctcggtgatc
    57781 gccaccgcgc cgcagaagcg cctggcgatc aagaccttcg tccagccctc cagtcgcggc
    57841 ctgatccgcg aggcggtgct gcgcgagttc aagcgcggcg gccaggttta cttcctgcac
    57901 aacgaggtcg acaccatcga caacatgaag gacgacctcg aggagctgct gcccgaggcg
    57961 cgcatcgtcg tcggccacgg gcaattgccc gagcgcgagc tcgaacgcgt gatgcgcgac
    58021 ttcacccagc agcgcgccaa cctgctgctg tgcaccacca tcatcgagac cggcatcaac
    58081 atccccaccg ccaacaccat catcatcaac cgcgccgacc gcttcggcct ggcgcagctg
    58141 caccagctgc gcggccgcgt cgggcgcagc caccaccagg cctacgccta cctgctgacc
    58201 cagccgggcg ccaagcccag cgcgcaggcg cagaaaaggc tcgaagccat tgcgatgatg
    58261 gaagacctcg gctccggctt ctacctggcg atgcacgacc tcgagatccg cggcgccggc
    58321 gaggtgctgg gcgagaacca gtcgggcgag atccagcagg tcggcttcag cctgtacacc
    58381 gagatgctca agcgtgcggt caaggacctg caggccggca aggagcccga cctgtcgcag
    58441 ccgctggagg tcgtctccga gatcaacctg cacaccccgg cgctgctgcc caccgactac
    58501 tgccccgacg tgcaggagcg cctgacgctg tacaagcgcc tggccaactg cgagatggaa
    58561 gaagatttgc gtgcgctgca ggaagagctc atcgaccgct tcggcgagct cccggcgcag
    58621 accgccgcgc tgatcgagac ccaccgcctg cgcatgctgg tgaaggacta cggggtgcac
    58681 aagctcgatg cgtccgatgc gcagatcagc gtgcagttcg ccaaggacgc gccgatcgac
    58741 ccggtcaagg tcatcttcct gatccagaag gaccgcagca ccaagatggc cggccccgag
    58801 aagctcgtgc gccgcgccag cctgcccaac ctcaaggagc gcgtgaaggc ggtgaaggac
    58861 ctgctcgagg cggtgaaggc ctgatccggc gaaggcgcaa gcgacccacg accatgctca
    58921 tcaactgcat cgcctaccag cacggccgca agctcgccga catcccggtc gaggacatca
    58981 gcgagtacat ccagcaaccc gagaccttcg tctgggtcgc gctcaaggac gccaccgccg
    59041 aggaactcgc ggtgatgaag gaggagttcg gcctccacga gctcgccgtc gaggacgcca
    59101 accaccgcca ccagcgcccc aaggtcgagg agtacgacga cgagctcttc gtcgtcatgc
    59161 acctgatcga ggagcgcgag ggcaagctcg aggtcggcga ggtgcacgtc ttcgtcggga
    59221 ccaactacat cctgtcggtg cgcaacaacc acgcccgcga cttcctcggc gtgcgcgcac
    59281 gctgcgagcg cgagccgaag atgctcgcca agggcccggg ctacgtgctg tacgcgctca
    59341 tggacgcggt ggtcgacggc tacttcccgc tcatcgacaa gctcgagacc gaggtcgagg
    59401 cgctcgagga gcgcatcttc accaagggtg cgggcaacac caacatgcgc cgcctctatc
    59461 gcctcaagcg caaggtcacc ctgctcaagc acgccgtgct gccgctgatg gaggccgccg
    59521 gcaagctgca cagcgggcgt gtgcccgagg tctgcgccaa gagccggcac tacttccgcg
    59581 acgtgtatga ccacctcgcc cgtatcgacg cctcgctcga cagtgtccgc gagaccatcg
    59641 gcaccgcgat ccaggtgaac atctcgctgg tcaccatcga ccagaccgag gtcagcaagc
    59701 gcctcgcggc ctgggccggc atcttcgcgg tggccaccgc cttcgcgggg atctggggca
    59761 tgaacttcaa ccacatgccc gagctggagt gggagtacgg ctacttcgcc ggcctcggca
    59821 tcatcgtcag cgccagcacc ttcctgtggt ggcgcttccg caaggcaagg tggctgtagc
    59881 gcgcagccga tgcgtaggag cgacgcaagt cgcgattgcc gccgctccgt cccgccgaca
    59941 ccccttcccg ctccaccgcc cgatcgcgac tcgcgtcgct cctacaaatc cccggcacgc
    60001 cggccgatcg cgactcgcgt cgctcttcca aatcaccgct tggcgtgccg taggagcgac
    60061 gcaagtcgcg attgcagcgc ctccgtcccc atcgacgccc atcgcccctg ccctgcccca
    60121 gacctttcct ccgcgccgct acaatccccg catgtccttc gacgagcgaa tgccatgagc
    60181 ttcttcgacg acctcgcccg cagcctcgaa cgcaaccgca tcacccgccg ccaggcgctg
    60241 tggctgctcg gcgcgggcgc ggcggccggc ctgtcgggct gcgccacctc cccggtcacc
    60301 ggcgagacca tcctcgtcgg catgagcgag gcgcaggaga agcagaccga cgcacaggtc
    60361 gcgccgcacc agttctcgca ggacctcggc gccatccagg acgaggcggt caaccgctac
    60421 gtcgccggca tcggccagcg catgggcacg ctcacccacc gtccgcagat gccctactcc
    60481 taccgcgtgc tcaacgccaa ctacgtcaac gcctacacct tccccggcgg cgcgatgggt
    60541 gtgacgcgcg gcatcctcgc cgacctcgac gacgaggccc agctcgccgc cctgctcggc
    60601 cacgagctcg gccacgtcaa cgcccgccac gccgcccagc gccagggcca gaacctggtc
    60661 gcgcaggcgg cgctcgccgg gctcaacgtg gcggcacaga gctccgactg gggcgggctg
    60721 atgagcatgg gcggacagat cggcgccagc gccctgctcg ccggctactc gcgcgagcac
    60781 gagcgcgagg ccgacgcgct cgggcaggaa tatctcgtca aggccggcta cccggcgacc
    60841 ggcatggtgc gcctgcacca gttgctggtt gccgaggaaa aatccgcccc ctcgctgctg
    60901 cagacgatgt tctccaccca cccgatgagc agcgagcgca tgcaggccgc gcaggccgcg
    60961 gccgacgcgc gctaccgcat cagcaacagc ctggacgccc gccgcgagcg cttcatggac
    61021 agcaccgcca gcctgcgccg catccgcccc accatcgacg cctgcaagaa cggcgaaacc
    61081 gcgatggccg ccaggcagta ccccaaggcg caggccgaat tccagaccgc gctggccagg
    61141 accccgcgcg attacgccag caacctgcgc atggcccagt gcctgcaggc ccagggccag
    61201 accgcgaagg cggtggacta cgccgacaac gcgcgcgaga tctacccgca ggaggcgcag
    61261 gcctacaagc tcgccggcgt gctcgccctg cagcagcgcg acgccggccg cgcctaccag
    61321 aacctcgacc gcttcgaccg cctgctcccc ggcgacgccg gcatcacctt cctgaagggc
    61381 atctcgctcg aaggcatggg caaccgccag gccgccgccc agcactacgc cgcctacctg
    61441 cgccagagcc agcagggcaa cgccgcgcag tactcgtaca accggctcaa ggcctggggg
    61501 atggtgaagt agcgcaggag cagcccgccg cgatcccgcg cctgcgagcc accatttcaa
    61561 cggaagggca ggatgaaggt gcccttctca cgcaccgcaa ggcttgcgtc gtgcagcgcc
    61621 accgtctcga acacgatcgg ctgcacgccg gacggcatgc cttcgaacgg ggatgacagg
    61681 gtgagcgtga cgctctgcac ctggcccggc ccgaccgtga attcgccggc gccctcgatc
    61741 cgcagcccgg gcaggccgct cacgccgatg cggaactggc gggtcgtctc ttccatgttg
    61801 atgagcttga gggtgtagct gttctcgatc cggcccgcgc tcgtctcctg catgagggcg
    61861 ccgcgatccc ggtgcacttc cacccgcaac agcacgcgct cgctcagtgt ccacaccccg
    61921 agcgcggcga acacgagcag caggccgaca tagaccaggg tccgcggccg cgaccaggct
    61981 ggggcaacct gtgcctggcc ggcaagctcg cgctcgctca tgaagcggat caagccgcgc
    62041 ggtgtgccga tcctgtccat gacctcgtcg caagcgtcgg cgcacaaccc gcagtcgatg
    62101 cactggtatt gcagtccgtc gcgaatgtcg atgcccgtcg gacacacctg cacgcagatt
    62161 ccgcagtcga tgcagtcgcc cttgtcgccc gactgccgca aggcgccgcg gggctccccg
    62221 cgcttgacgt cgtaggcgac gttcgccgta tccggatcga tcatcacgcc ctggaagcgc
    62281 gagtacggac acatgtgctg gcatacggcc tcgcgtgcga gcccggcctg cagataggtg
    62341 aagctcgcgt agaaaagcag ccagaaggtt tcccacgggc cggtctggag gccgacgatt
    62401 gccggcagca gtgcgcggat gggagtgaag tagccgacga agctgatcgc ggtccacgcc
    62461 gcgaccagcg cccagatgcc gtgcttgacc gacttgatga gcagcttgcg cgccgacatc
    62521 gggccctggt cgaggcgcag gcgcgcgagg tggtcgccct cgatcctggc ctcgatccac
    62581 acgaagatcg acgtatagac cgtctgcgga caggcaaagc cgcagaacag gcgcccggcg
    62641 atcgcggtgg tgaagaacag cgcggtcgcg gccaggatca ggacgacggc cagcagcagc
    62701 gcgtcctgcg gccacaggac caggtcgaac aggtagaact tctcgtcgac gatgtcgaac
    62761 aggatcgcct gccgcgtgcc ggtctccgtc gtccacggca accagcacgc gccgtagaac
    62821 acgacctggg tgagccagac catggcccag cgcaggctgt tgaagcggcc ctgcaccgcc
    62881 ttggcgtgga tctttccctt cttgcgatag aactccagct tggcttcgcg cacc
//
LOCUS       DAOQBS010000003        60611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_194_length_60611_cov_13.817623, whole genome shotgun sequence.
ACCESSION   DAOQBS010000003 DAOQBS010000000
VERSION     DAOQBS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 60611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60611
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194_length_60611_cov_13.817623"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKN25_00620"
     CDS             <1..500
                     /locus_tag="PKN25_00620"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNV89427.1"
                     /translation="AQDYAMANRQAMLELVLAGLARHLPPFTVTTEAVNCHHNYVARE
                     HHFGADVWVTRKGAIRAGEGELGIVPGSMGARSYIVRGRGNAESFCSSAHGAGRRMSR
                     TAATKRFTEADLARQTKGVICRKDKGVVDEIPGAYKDIDTVMANQSDLTEVLHTLKQV
                     VCVKG"
     gene            900..1388
                     /locus_tag="PKN25_00625"
     CDS             900..1388
                     /locus_tag="PKN25_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89428.1"
                     /translation="MSTIGYAQLISRLCLVVRPLAKPAQISGSVNRPVDSADRVLLPC
                     GVAIEGTLVGHLEFALRHEGVNLDVIDALFEHLTPEDLLARLNAAPNGAHIRRACHLW
                     EWLTEPRTAGSCLADGRLRRPVSCPMTTSPPGSPPTTVDSACATMHWGPPVSPRWCGA
                     RQ"
     assembly_gap    1597..1696
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1927..3627
                     /locus_tag="PKN25_00630"
     CDS             1927..3627
                     /locus_tag="PKN25_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HNV89429.1"
                     /translation="MQPGTLAFLAFSPILLAAVLLVGLRWPAKRAMPVVYFLTAGIGL
                     YVWEMSLNRVLASTLQGLVITIGVLWIIFGAILLLNTLKYSGGITAIRAGFAKISPDR
                     RIQAIIIGWLFGCFIEGASGFGTPAAIAAPLLVAIGFPALAAVLLGMLVQSTPVSFGA
                     VGTPIIIGVNSGLDSATIGAQLAARGSSWDVFLQVITSNVAIIHAMVGTVIPLIMVMM
                     LTRFFGREKSWKAGFEVLPFAIFAGLAFTVPYAFTGVFLGPEFPSLLGGLVGLALVTT
                     AARFGFLVPKKTWDFADPKEWPSEWMGSVEMKLDQLPRHQPISIFRAWLPYVLVGALL
                     VLSRVFPEIGGAMKSVVIVFADILGEKGIKADFQPLYLPGGILVAVVLITVFLHGMHA
                     RSLVKAVGESSRVLLGAGFVLLFTVPMVRILINSGVNAADLPSMPIAMAKWVADSVGN
                     VYPLLAPSVGALGAFIAGSNTVSNMMLSQFQFGVATSLGISTAMVVAVQAIGAAAGNM
                     VAIHNVVAASATVGLLGREGAILRKTFWPTVYYVLMTGTVAMIAIYVLGVSDPLLAAS
                     "
     gene            3741..4574
                     /locus_tag="PKN25_00635"
     CDS             3741..4574
                     /locus_tag="PKN25_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNV89430.1"
                     /translation="MSPLSSAPANATRVAPPLEAARSYPAKPADVYLFGTCVIDVFFP
                     GAGMDAIRLLEREGIRVHFPQAQSCCGQPAYTSGYTDEAREVARAQLALFAGDWPVVI
                     PSGSCAGMFRHHYFELFKDEPETLQQVASLSARTFELAEFLLEVCKVRFEHTGAPVRL
                     ALHTSCSARREMNTHLHGRALLAQLAGVERVEHDHESECCGFGGSFSVRMPEISGAMV
                     QDKTAALKGSGAAEVVSADCGCLLNINGCFEKQGDTLRGEHLASFLLRRTTSASTGGV
                     R"
     gene            4571..6010
                     /locus_tag="PKN25_00640"
     CDS             4571..6010
                     /locus_tag="PKN25_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HNV89431.1"
                     /translation="MSHVADTPAQPIAFTRNAREALADAQLRRNFRGAMDALMDKRRN
                     QFPDGDELERLRAFGNRVRARALSKLPDLLERLESNLVRNGVQVHWAETVEEANAIVH
                     GIAERHAARKVIKGKSMVSEEMEMNHYLGARGVDCLESDMGEFIVQLRAEKPSHIIMP
                     AIHLNAGQVAKLFHDKLAVEYTEDVDRLIQIGRETLRRNFFEADIGVSGVNFAIAETG
                     TLLLVENEGNGRMSTTVPPVHIAVTGIEKVVENLRDVIPLLSLLTRSATGQPITTYVN
                     MISGPRKADELDGPQEVHLVLLDNGRSQAFADGELRQTLNCIRCGACMNHCPVYTRIG
                     GHAYGTVYPGPIGKIVTPHMLGLETTRDLPTASSMCGACGEVCPVKIPIPSLLRRLRE
                     EAVRAPAAEPHHVRGQGAKHSRKETLVWRAWRTLNTSPGLYRAAMYAGTRLRGLMPAD
                     IGPWTRYRSAPKPAARSLHELAREHLGEQ"
     gene            6034..6705
                     /locus_tag="PKN25_00645"
     CDS             6034..6705
                     /locus_tag="PKN25_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNV89432.1"
                     /translation="MNAKERILGKLRASLAGTTPVADDYDIGLVTDPWRYAADERIAR
                     LRRMLEAVHGETLVTTVAQWPQRVLEALAARGIDELLVSPATAHGRRLAEHVRAQDAA
                     LRLKGYENPVEAWKDELFFHTPASLTGTLGGIAATGSLVLWPSPQEPRLMSLVPPLHI
                     ALLNASEVRDNLFELMHAQRWTDGLPTNALLVSGPSKTADIQQTLAYGAHGPKELLVL
                     VIEDA"
     assembly_gap    6808..6907
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7018..9723)
                     /locus_tag="PKN25_00650"
     CDS             complement(7018..9723)
                     /locus_tag="PKN25_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HNV89433.1"
                     /translation="MSFRHLKSLFNPRSIAVIGASARERRMGNVLMRNLLSGQFAGPI
                     MPVNPRRTSVAGVLTYPSIAALPQTPDLAIICTPAPLVPRLVEELGERGARAVMVMAN
                     QLDTTLGADGRPIERSILEITRRYEMRLLGGGTLGILVPGLGLNATFSQIAARPGKLG
                     FVSQRDTVGTMVLDWALRKKVGFSHFVSLGDSLDIGFGEVLDFLAADSGTRAILLYIE
                     SIQDRRAFMSAARAAARNKPVIAIKAGRSPDNPLMGVSDPLFLEMPNLVSHDDVHDAA
                     LRRAGILRVDQLEEMFGAAETVLRARPLRGNRLVVLSNGGGAGLMVEDSLYLAGYSLP
                     ALKEQTIERLRRFMSPAWNGRNPLEIRVDSEPACYERLLKILQEERDGDAVLLIHTPN
                     ALSSSIDTAQQVIATARETGINLMTCWVGDESVEKERNLFINAGIATFDSPEHAASAF
                     LHMHRHRIASEVLMEVPTSEPEAFKPDAARARAVIQGALAQSRASLTESESKDVLAAY
                     GVPVIQTHLAATPEEAAQIAGRIGLPVALTLMSRDIRRKWDVGGVALNLESTEAVQVA
                     ARGMIERAGRAEPPVSVSGFTVQRMVMRGHARQLLIGVSTDRLFGPVIVFGEGGRALE
                     IFRGLAVGLPPLNKPLADDLITRSKAAALLEARGHLPAADRDALALTLTKVSQIVVDH
                     PEIHEMDINPVFVDEHGVQALDAHMRLRPATAHEHRLAIQPYPKALEEPSRLRDGRPI
                     LLRPIRPEDEPAHYAFLSRLTREDFIYRFFNYIPEFPRREMARLTQIDYDREMAFIAT
                     ATGPDGKPETLGVSRAVADPDNHTAEFALVIRSDLKRQRLGSILMDKLVRYAREIGIQ
                     RLVGEVMGENEPMLGLLKHLGFKLKASEEPGVVRASFELRPPSEG"
     gene            complement(9864..10568)
                     /gene="ubiG"
                     /locus_tag="PKN25_00655"
     CDS             complement(9864..10568)
                     /gene="ubiG"
                     /locus_tag="PKN25_00655"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HNV89434.1"
                     /translation="MNTVNADPAELQKFSDLAHRWWDPASEFRPLHEINPLRLGWIDG
                     KAALAGKKVLDIGCGGGILAEGMAALGAEVTGIDLSEKALSVARLHLFESGHKVDYRH
                     MSAEALAEESPGRFDVVTCMEMLEHVPDPASIVTACARLVKPGGHVFLSTLNRNPKSY
                     LFAIVGAEYLLKLLPAGTHEYARFIKPSELSRHCRNAHLDVQEIIGLSYNPLAKTYSL
                     GRDTDVNYLMHTVAAG"
     gene            complement(10676..11374)
                     /locus_tag="PKN25_00660"
     CDS             complement(10676..11374)
                     /locus_tag="PKN25_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV89435.1"
                     /translation="MIKQSKKQMLMLAAIASIGLSAPSAFAQVSDVVVAGKGEIPYVI
                     DARNVVARSGTGLCWRTGYWSPAAASTALAGDLPVGCACDKDIVPADKCTVKAAAAAP
                     GPKPTADKVKLSADALFDFDKAVLKAEGKAKLDQLAAQAKSLKLEVILAVGHTDRLGS
                     NTYNQRLSERRAAAVKTYLVSKGVDANRVYTEGKGETQPVTGNKCDKVGGRAAQISCL
                     QPDRRVEVEVIGSK"
     gene            complement(11475..12794)
                     /locus_tag="PKN25_00665"
     CDS             complement(11475..12794)
                     /locus_tag="PKN25_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HNV89436.1"
                     /translation="MSEAVDLLINARWIVPVEPRDAVLADHAVAIRGGRIVDLLGQDE
                     ARTRYVADEVVELPRHLLIPGLVNLHTHAAMSLLRGIADDLPLMRWLEEAIWPAESRH
                     VSAAFVRDGTLLAAAEMIRGGITTCSDMYFHPEAAAEAFAAAGMRAVVGAVVLEFPTS
                     YASDPEDYLRKGLAARDRWQGHPRLGFSIAPHAPYTVSDDSFHQVQTLADELGLPIHV
                     HIHETAQEIADSLAVHGCRPLARLARLGVLGSNLIGVHAVHLDEADIELLARHGCSVA
                     HCPTSNMKLASGIAPVPRLLAAGVPVGLGTDGAASNNRLDLLQEMRHAALLAKVGSLD
                     ATAVPAHAALRMATLGGARALGMDDRIGSIEKGKCADLCALDLSAPQCRPCFDPVSHL
                     VYVCGRENVSHVWIDGETRVDKGVSLLHINDTELLRLVSMWQTKLGN"
     gene            12964..14328
                     /locus_tag="PKN25_00670"
     CDS             12964..14328
                     /locus_tag="PKN25_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNV89437.1"
                     /translation="MNRIPERIGKYRIIKEIGRGATAVVYLAETPDYPEPVALKHVRF
                     DDKARDEAKWNRRLIKLLKAERVVSGRLDHPNIIRIHDASIEPDEAYVVMEYFPGGSL
                     ERYCSFQNLLPIHRTIGIVFKCCMALDHAYRQGIVHRDIKPANILVDEQDNVKITDFG
                     LALNINKKLDTDSTFIMGVGSPAYMSPEQIKGYPLNQKTDLYSLGVLLFHMLTGRLPF
                     RASNPAQLVYKIINADPPSVSQLNPDVPEQMDAVIRKALEKDLYSRYRNGAEFAKDLA
                     AVRYQILDEGHVHHDAERFARLRKLAFFTEFEDVDLWEVMRICAWRKVGSDLGLMREG
                     DLDQRFGIVLEGKVELSIGGRRVLEVGPGEVFGEQAWLDQLEHKQLLSAVSLDELSYL
                     EVNPAALALASEEVLERFRRQLGTVAVRRLAAVSRQAAEHAPAAVRAVRAAPVVHDLQ
                     IVDY"
     gene            complement(14416..15513)
                     /gene="hemH"
                     /locus_tag="PKN25_00675"
     CDS             complement(14416..15513)
                     /gene="hemH"
                     /locus_tag="PKN25_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNV89438.1"
                     /translation="MPRYRPEPIHRHDSTPRTGVLLVNLGTPAAPTAAALRPYLKQFL
                     SDPRVVEIPRLVWWPILNGIILNTRPKASAQKYASIWTDEGSPLLVHTVRQTKLLAGY
                     LAKDGYDGLDIDYAMRYGAPAIGDTLDAMRARGCTRILVVPMYPQYSASTTATVVDEV
                     GSSLARWRNQPEMRFVRNFHDDPGYIDALVKSVREHWAANGEPERLLLSFHGVPRRSL
                     DLGDPYHCECHKTGRLVAEGLGVARERVLVTFQSRFGKAEWLKPYTQPTLEQLAAEGV
                     RRVDVMCPGFVADCLETLEEIALECKEAFLEKGGQAFHYIPCLNERDAWIKALAALVR
                     KHLGNWLEIAAPDAAARSAALERARTLGAGQ"
     gene            complement(15565..16593)
                     /gene="hrcA"
                     /locus_tag="PKN25_00680"
     CDS             complement(15565..16593)
                     /gene="hrcA"
                     /locus_tag="PKN25_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNV89439.1"
                     /translation="MNPLDARSQILLKTLIERHIADGQPVGSRALSRYSGLELSPATV
                     RNVMSDLEEMGFIASPHTSAGRIPTALGYRFFVDSLLTVQPLENARVRALEGQLLPNQ
                     PQRLISSASHLLSELTQFAGVVVAPRKDAVRIRQFEFISLAENRILLIIVTTGGDVQN
                     RLLITRRAYTPTELNGAAAYLNEHFAGLSFEEIRRRLGDELRQLRSDMSELMTATVDA
                     GSEAAEDNAASYVLSGETKLLDVEDLSSNMSQLRELFRLFEQRTGLLQLLDLSNRAEG
                     VQIFIGGESGLAPLDGCSVITAPYEVDGQVVGSLGVIGPTRMAYDRVIPIVDITARLL
                     SNALSSKH"
     gene            16713..17594
                     /locus_tag="PKN25_00685"
     CDS             16713..17594
                     /locus_tag="PKN25_00685"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNV89440.1"
                     /translation="MSTPFRTLALIGKYQSPDVAESVLSIARFLRDRGLAVLIEQGTA
                     SSIGGAHDFPVASYEHIGASADLAVVIGGDGTMLHTARRLIEHGVPLVGVNLGRLGFL
                     TDIARSSATERLAEILDGAFTAEDRFMLDVEVLRGGARVFHTLALNDVVVNKGELGRM
                     IEFELSIDEEFVYTQRSDGMIVSTPTGSTAYALSANGPILHPSVGGIALVPLCPHALT
                     ARPITLPDSCRIDIVLLPPHDARVHFDGQTRFDLRAGDCVRMTRSSRSLRLLHPEGYS
                     YFAMLRQKLHWSAAPRV"
     gene            17677..19332
                     /gene="recN"
                     /locus_tag="PKN25_00690"
     CDS             17677..19332
                     /gene="recN"
                     /locus_tag="PKN25_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNV89441.1"
                     /translation="MLRRLTIRDFVIVDRLDLEFAAGFGALTGETGAGKSILLDALGL
                     ALGGRGEAAMVRSGRERADVAAEFDLPAGEGLAAWLVEQELPADEGMLILRRTIDMGG
                     RSRAWINGVAATLAQLRAAGEWLADIHGQHAHHALLRTDAQRALLDAQAGAGALVAEV
                     AARHREWQRLLRLQREAEADSASSARERELLAWQLRELEQVGFDIEEWAEINAEHARL
                     AHAASLIEGAEAAVVALGEGELAVCSVLGRLDGRIAALADFDAGLADVRELISAAAIQ
                     ADEALHALRRYRDRLDLDPGRLAELERRIAAVMEAARKYRVQPEELPALAQQWRERLD
                     TLEATADPARLAAAAQAARAAFESVAAELTAARLPAARRLSEEIAAAMQDLAMAGGRF
                     EVALTPCAPSAAGVEEVEFRVAANAGQPLRSLAKVASGGELSRIGLAIQVITSRDSAV
                     PTLIFDEVDVGIGGRVAEIVGKLLARLGQDRQVLCVTHLPQVAACADWQWRIAKREQG
                     GETLSEVAPLDDAARVEEVARMLGGVNITATTREHAAEMLGRR"
     gene            complement(19401..19841)
                     /gene="fur"
                     /locus_tag="PKN25_00695"
     CDS             complement(19401..19841)
                     /gene="fur"
                     /locus_tag="PKN25_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HNV89442.1"
                     /translation="MSDNSRNLKSIGLKATYPRLKILDLFQSSEQRHLTAEDVYRTLM
                     NEGMDIGLATVYRVLTQFEQAGLLERHYFESGKAVFELAEGGHHDHLVCMQCGKVEEF
                     FDAEIERRQNQIALERGFAIREHALYIYADCQRENCPNLGREKD"
     gene            20091..20498
                     /gene="bamE"
                     /locus_tag="PKN25_00700"
     CDS             20091..20498
                     /gene="bamE"
                     /locus_tag="PKN25_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNV89443.1"
                     /translation="MRSLLAALIVSSGLLAGCSFDSVVGLVDPYRIDIRQGNYVDQEM
                     VAQLRRGMTRDQVRYVLGSPLVVDMFRKDRWDYVYRFRPGSGEAQQRVISLFFTGDVL
                     DRIEGDVAAASGTPAAAAQERSRVVEIPPASAR"
     gene            20565..21371
                     /gene="dapB"
                     /locus_tag="PKN25_00705"
     CDS             20565..21371
                     /gene="dapB"
                     /locus_tag="PKN25_00705"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNV89444.1"
                     /translation="MSQIRVAIAGASGRMGRMLIEAALKEEGVVLAAAFDRSDTPFIG
                     RDAGEMAGVATGVLITDDAAAAIGAADCVIDFTRPEGTLAHLALARAAGKAMVIGTTG
                     FDAAGKTAIAAAAEHVPVVFAPNMAVGVNAVFRLLEVAARILSEGYDVEVIEAHHRFK
                     VDAPSGTALRMGEVVARELGRDLETCAVYGREGHTGERSAETIGFSTIRGGDVVGDHT
                     VLFAGIGERIEITHKSGSRMPYALGSMRAARFLAGRAKGLYDMQDVLGLR"
     gene            21380..23881
                     /locus_tag="PKN25_00710"
     CDS             21380..23881
                     /locus_tag="PKN25_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV89445.1"
                     /translation="MSGAAESRGSPPDPGSVEAELSRLRAERDAALRSLARARRHLDL
                     VSANAGAGVWYMDLRGGSIRLDERWRRLLGYDGGDAGDWMALVDDEGRAALQQALRAH
                     LGGEAAELRAEIRIRAQDASWHWIEVCGNASGRGEDGRWARVEGTYRDVTERKLREIE
                     LLQAKEAAEAASRAKGDFLANMSHEIRTPMNGIIGMTDLLLDSTIAGEQREYLQTVKS
                     SAEALLTIINDILDFSRIEAGRLSVEQIDFSLHSVVSETCRALALRASQKGLELYYDV
                     APDVPSVLRGDPTRLRQILVNLIGNAIKFTEHGEVEVRVRCVQRNGGAASIEFLVRDT
                     GCGIAPDKLEAIFGAFSQADNSTTRKYGGTGLGLAICRQLTELMGGRIGVDSVPGAGS
                     RFEVTLPLRAVADPTPLRVGALAGTRVLVASANAAFADFLCATLQEAALRAEAACSGV
                     AVEQALARAADGSDPFAFLLMDADLPEPGGFALAQRFAAAWPRPDRIIPMMASHSQRN
                     DALRCEELGLGYRLAKPFSAEDLLAVLRMAQGGEREAAEPPPFELAPVELRSTVGMEP
                     PARVLEVLVVEDNPVNQTVAKRMLEHVGHSVTVANNGEEALEAIDNRHFDVVLMDVQM
                     PVMGGIEATQAIRAREARRSWVLAGDWKPVPIIAMTAHAMEGDRERCLQAGMDDYVAK
                     PVRPEALFAAIERVLGPADADEAAGDITLLELDPADRRRIACLDDARAMFAGEDGVVE
                     QLVEVFLRDLPRTVADLHKGAADMDFARLRELGHMIKGSVGVFSAQRAVDAARRLEEL
                     AAAGDPRALTSQAGLAAREIELLGEVLREETACGR"
     gene            24042..25184
                     /gene="carA"
                     /locus_tag="PKN25_00715"
     CDS             24042..25184
                     /gene="carA"
                     /locus_tag="PKN25_00715"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNV89446.1"
                     /translation="MSAFPPALLALADGTVFHGKGIGAVGSTVGEVVFNTSMSGYQEI
                     LTDPSYCRQIVTLTYPHIGNTGINPEDIESSRVQAAGLVIRDLPILPSNFRMTQRLDA
                     YLRAEKVVAIAGLDTRKLTRVLREKGAQAGCIVTAAAGQALDAEAAVAQARAFPGLAG
                     MDLAKVVSVAERYEWKQAEWALGSGYSDRADGRFHVVAYDFGVKYNILRMLVERGCRL
                     TVVPAQTPASEVLALNPDGVFLSNGPGDPEPCDYAIAAIRELVAARMPTFGICLGHQL
                     LALASGAKTMKMPTGHHGANHPVKDLDTGKVLITSQNHGFAVDPATMPANLKVTHVSL
                     FDGTNQGIARTDVPAFSFQGHPEASPGPHDVAYLFDRFIGLMEAAK"
     gene            25270..28500
                     /gene="carB"
                     /locus_tag="PKN25_00720"
     CDS             25270..28500
                     /gene="carB"
                     /locus_tag="PKN25_00720"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNV89447.1"
                     /translation="MPKRTDIKTILIIGAGPIIIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPETADVTYIEPITWQVVERIIDKERPDAILPTMGGQTALNCALDL
                     GRHGVLAKYGVELIGASEEAIDKAEDRLKFKDAMTRIGLGSARSGIAHSMEEALQVQG
                     GIGFPVIIRPSFTLGGTGGGIAYNMEEFQEICKRGLEASPTNELLIEESLLGWKEYEM
                     EVVRDRADNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQIMRNASIAVLREIG
                     VDTGGSNVQFAINPQDGRMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LRNDITGGATPASFEPSIDYVVTKIPRFAFEKFPQANDRLTTQMKSVGEVMAMGRSFQ
                     ESFQKALRGLEVGVYGLDEVEADREDLEHEIASPGAQRIWYVGQAFREGMSLEQVFNL
                     SKIDPWFLAQIEDIVLSEKALAGRSLKALQAAELRELKKKGFSDRRLAKLLNADETAV
                     RLHRHTLGVRPVFKRVDTCAAEFATSTAYLYSSYEDECEAMPTERKKIMVLGGGPNRI
                     GQGIEFDYCCVHAALALREDGYETIMVNCNPETVSTDYDTSDRLYFEPITLEDLLEIV
                     HIEKPVGVIVQFGGQTPLKRAQALEANGVPIIGTSPDMIDAAEDRERFQQLLVELGLR
                     QPPNRTARTPAEAVRLAAEIGYPLVVRPSYVLGGRAMEIVHEQKDLERYMREAVKVSN
                     ESPVLLDRFLNDATEVDVDALSDGKQVMIGGIMEHIEQAGVHSGDSACSLPPYTLSAR
                     LQDELRRQTEAMARALKVVGLMNVQFAIQGEGDNAVVYVLEVNPRASRTVPFVSKACS
                     LPLAKIAARCMAGQSLAEQGVSGEIVPPYYSVKEAVFPFVKFPGVDTILGPEMKSTGE
                     VMGVGRSFAEAFVKSQMGAGVRLPTAGTVFISVRPTDKPVAIEVARELHEMGFALVAT
                     RGTAAAIEAAGIPVGVVNKVNEGRPHIVDIIKNEEIVLVINTVDEKRQAIADSRSIRT
                     SALAAKVSIFTTIEGARAACMGMRHLSNGLEVYSVQGLHAELKQQA"
     gene            28497..28973
                     /gene="greA"
                     /locus_tag="PKN25_00725"
     CDS             28497..28973
                     /gene="greA"
                     /locus_tag="PKN25_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNV89448.1"
                     /translation="MNKTPLTVAGAEKLRTELHRLKTVERPNVIAAIAEARSHGDLSE
                     NAEYEAAKDRQGFIEGRIMEVESKLANAQIIDPTLLDADGRCVFGATVELEDLESGQV
                     VTYQIVGDDEADIKDNKISISSPIARALIGKYAGDIAEVQAPGGVREYEILDVRYV"
     gene            complement(29085..29537)
                     /gene="yhbY"
                     /locus_tag="PKN25_00730"
     CDS             complement(29085..29537)
                     /gene="yhbY"
                     /locus_tag="PKN25_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HNV89449.1"
                     /translation="MTELSPAERSELRARAHHLNPVVTVAGNGLTPAVLAEIERALQA
                     HELIKVKVQGAEREQREALMQELCAAVDAAPVQHIGSILVVWRARREDDKSSAPAEKK
                     PVRASTAKSAAAFAAAARRAALAKASAESRRSPARAPAPRGRGAARGR"
     gene            29620..30243
                     /locus_tag="PKN25_00735"
     CDS             29620..30243
                     /locus_tag="PKN25_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HNV89450.1"
                     /translation="MKKNKTSRAWVHDHVNDPWVQRAQAEGYRARAAFKLLEIDERDH
                     LLRPGAVVVDLGAAPGSWCQLAVKRCGPKGRIFALDLLPMEPVAGVEFLLGDFTEDAV
                     LEEFEARLGGASVDVVLSDMAPNLSGVATVDQARSVHLCELALDFAVRHLKPGGQFVV
                     KVFQGEGFMEYRKQMEAAFVSVQVRKPKASRDRSAEVYLVGRGPRRP"
     gene            30366..32258
                     /gene="ftsH"
                     /locus_tag="PKN25_00740"
     CDS             30366..32258
                     /gene="ftsH"
                     /locus_tag="PKN25_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNV89451.1"
                     /translation="MNNLFKNLAIWMVIGVVLMTVFNQFNTRQTPASTMEYSQFLEEA
                     KAGRIARATVDGRVLKATTQEGRTITVYTPGVQDIWMISDLMRYGVQINASKPEEEQS
                     FLASVFVSWFPMLLLIGVWIFFMRQMQGGGKGGAFSFGKSKARMLDESANSVTFADVA
                     GCDEAKEEVAELVEFLRDPSKFQKLGGRIPKGVLMVGSPGTGKTLLAKAIAGEAKVPF
                     FSISGSDFVEMFVGVGAARVRDMFEQAKKQAPCIIFIDEIDAVGRQRGAGLGGGNDER
                     EQTLNQLLVEMDGFEGQTGVIVIAATNRPDVLDPALLRPGRFDRQVVVALPDIRGREQ
                     ILKVHMRKVPIAPDVDPQVLARGTPGFAGADLANLVNEAALFAARANKRLVDMEDFER
                     AKDKIMMGAERRSVVMPEEERRNTAYHESGHAVVARLLDKTDPVHKVTIIPRGRALGV
                     TMQLPTEDRYSQDRDRLLQTIAVLFGGRIAEEIFMKQMTTGASNDFARATDLARRMVT
                     QWGMSDTLGPMVYGEEEGEIFLGRQVTTHRNVSEATMQKVDAEIRRIIDQQYSLARRL
                     LEENSDKVEAMTAALLEWETIDAEQVNDIMAGRPPRPPKPASAPSSRPKDDAPGAAPT
                     APAPAA"
     gene            32359..33189
                     /gene="folP"
                     /locus_tag="PKN25_00745"
     CDS             32359..33189
                     /gene="folP"
                     /locus_tag="PKN25_00745"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNV89452.1"
                     /translation="MSVLQCGRFRLDLSQPRVMAIINLTDDSFSGDGLRGDLARALAR
                     AERALEEGAEMLDIGAESTRPGSDPVPEAQELERVVRFVEAARGWKVPLSIDTLKPTV
                     MRAALAAGADMINDINAFRASGAIEAVAAGESGLCVMHMLGQPRTMQQDPRYADVVGE
                     VTGFLDERVAALVAAGVARERIVLDPGFGFGKSVGHNYALLRELARVGVGGLPVLAGL
                     SRKSMLGAVTGRAVGERLAASVAGALIAVQNGARIVRVHDVAATRDALAVWKATVTGC
                     "
     gene            33246..34598
                     /gene="glmM"
                     /locus_tag="PKN25_00750"
     CDS             33246..34598
                     /gene="glmM"
                     /locus_tag="PKN25_00750"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNV89453.1"
                     /translation="MARKYFGTDGVRGKVGDTPITPEFVMRLGYAAGVTLVAREKLPA
                     GEHPAVLIGKDTRISGYMLEAALEAGFAAAGVDVMLAGPIPTPAVAYLTRALRLQAGV
                     VISASHNPFYDNGIKFFSADGSKLPDAVEAEIEAHLDEPMGCVDSARLGKARRIDDAA
                     GRYIEFCKSTFPNELDLRGLRIAVDCAHGAAYQIAPKVFHELGAEIIPVGVEPNGLNI
                     NDGVGATRPENLRQAVLKHGADLGIALDGDADRLIMVDHRGEIYDGDKLLYVVACARH
                     AEGRLEGVVGTLMSNLGFEHAIQRLGTPFARAKVGDRYVLELLQERGWKIGGENSGHI
                     ICLDRHTTGDGIVSALQVLAALKQRGLSLADACADLVFYPQRLINVRMPAGFDWRADG
                     SIASAQARAEQTLGDSGRVLLRPSGTEPLLRVMVEGRDGELVERLAREIAGVVEEAIA
                     "
     gene            34751..34990
                     /locus_tag="PKN25_00755"
                     /pseudo
     CDS             34751..34990
                     /locus_tag="PKN25_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
     gene            35113..35850
                     /gene="tpiA"
                     /locus_tag="PKN25_00760"
     CDS             35113..35850
                     /gene="tpiA"
                     /locus_tag="PKN25_00760"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNV89454.1"
                     /translation="MTRLVAGNWKMNGSLAANRELLDGLVSIPGVEIAVCVPYPYLAQ
                     AQQRPEGVALGAQDVSEYAAGAYTGEVSAAMLAEFGCRYAIVGHSERRALFGEEDATV
                     GRKALAALQAGLVPIVCVGETLAERDEARAVEVIGRQLEAVREVLGAAAMGAVVLAYE
                     PVWAIGTGRSASVAQVAEVHAAIRAWLKVHGVAAGGVRILYGGSVKADNAAALFAVED
                     VDGGLIGGASLVAADFLAICRAAAGGV"
     gene            35883..36233
                     /gene="secG"
                     /locus_tag="PKN25_00765"
     CDS             35883..36233
                     /gene="secG"
                     /locus_tag="PKN25_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNV89455.1"
                     /translation="MGEYLFSLVLTVHVLVGLGVIGLVLVQHGKGADMGAAFGSGASG
                     SLFGASGSANFLSRTTAVLATVFFLTSLGLSFLASNKPAAPGSVMEGAQTAPATNPAP
                     AAPVESSPAQSIPK"
     gene            36299..36383
                     /locus_tag="PKN25_00770"
     tRNA            36299..36383
                     /locus_tag="PKN25_00770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36333..36335,aa:Leu,seq:gag)
     gene            36491..36865
                     /gene="ndhC"
                     /locus_tag="PKN25_00775"
     CDS             36491..36865
                     /gene="ndhC"
                     /locus_tag="PKN25_00775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNV89456.1"
                     /translation="MLENYFPVLMFMLVGLGFGLAPVILGRLLAPYRPDSEKNSPYEC
                     GFEAFEDARMKFDVRYYLIAILFILFDLEIAFLFPWATVFQEFIAAGEIAWFVFGSVM
                     VFLAILVIGYIVEWKNGALDWE"
     gene            36869..37345
                     /locus_tag="PKN25_00780"
     CDS             36869..37345
                     /locus_tag="PKN25_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNV89457.1"
                     /translation="MSIEGVFREGFVTTSLDAVINWTRTGSLWPMTFGLACCAVEMIH
                     AGCSRYDLDRFGVVFRPSPRQSDLMIVAGTLCNKMAPALRKVYDQMSEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGILQLQNKIKRTNTIAR"
     gene            37360..37965
                     /locus_tag="PKN25_00785"
     CDS             37360..37965
                     /locus_tag="PKN25_00785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNV89458.1"
                     /translation="MSAKLERLSQTLRDVFGNRIQSLVLDRGEVTIEVSAAEYPRVAR
                     QLRDHADLRFEQLIDLSGLDYSAYGNAIWEGKRFASVAHLTSVKHNWRLRVRVFADDD
                     AFPVIDSVVDVWPSANWYERESFDLYGILYSGHPDLRRILTDYGFVGHPFRKDFPVSG
                     YVEMRYDPEQGRVVYQPVSIEPRENTPRIVREEKYGDVGHG"
     gene            37958..39211
                     /locus_tag="PKN25_00790"
     CDS             37958..39211
                     /locus_tag="PKN25_00790"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNV89459.1"
                     /translation="MAEIRNYTINFGPQHPSAHGVLRLVLELDGEVVERADPHIGLLH
                     RGTEKLAETRTWVQSVPYMDRLDYVSMMCNEHAYCMAIEKLLGVEVPLRAQYIRVMFD
                     EITRVLNHLLNIGTHALDIGAMTMVLYTFREREDLMDVYEAVSGARMHAAYYRPGGVY
                     RDLPDRMPKYEVNKFKNEKTVRELNANREGSMLDFLEDFTNRFPTYCDEYETLLTDNR
                     IWKQRTVGIGVVSPEQALAWGFTGPMLRGSGIAWDLRKKQPYEVYDRVDFDVPIGKNG
                     DCYDRYLCRMEEMRQSNRIIKQCIDWLRKNPGPVITDNHKVAPPSREAMKTNMEELIH
                     HFKLFTEGMHVPKGEVYAAVEHPKGEFGVYAVSDGANKPYRLKLRAPGFAHLAAMDDI
                     ARGHMIADVVAIIGTMDVVFGEIDR"
     gene            39300..39779
                     /gene="nuoE"
                     /locus_tag="PKN25_00795"
     CDS             39300..39779
                     /gene="nuoE"
                     /locus_tag="PKN25_00795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNV89460.1"
                     /translation="MLSQESLQQIDREIAKYPPDQTQSAVMAALRIAQVEKGWLPKEL
                     IEFVARYLGMPPIAAFEVASFYNMYDLAPVGRHKITVCTNLPCALSGGVHAADYIKEK
                     LGIDFNETTPDGKFTLKEGECMGACGDAPVLLVNNHHMCSWMTTEKIDQMLAELDKK"
     gene            39791..41095
                     /gene="nuoF"
                     /locus_tag="PKN25_00800"
     CDS             39791..41095
                     /gene="nuoF"
                     /locus_tag="PKN25_00800"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNV89461.1"
                     /translation="MILAGCDGDRTWRLQDYVARGGYSALRKILAEKIPQDAIIAELK
                     TSVLRGRGGAGFPTGLKWSFMPRSFPGDKYLACNSDEGEPGTFKDRDILRYNPHSVIE
                     GMAIAAYAMGAARGYNYIHGEIFETYSRFEEALAEAREAGFIGRNILGSDFSFELFAH
                     HGYGAYICGEETALLESIEGKKGQPRFKPPFPASYGLYGKPTTINNTETFASVPFIMN
                     MGGEAFLNLGKPNNGGMKLFSVSGHVNRPGNYEIPLGTPFAELLEMAGGMRGGRKIKA
                     VIPGGSSAPVVPGGVMMDCTMDYDSISKAGSMLGSGAVIVMDETTCMVKALERLSYFY
                     FEESCGQCTPCREGTGWLYRVVHRIEHGLGRPDDLDLLNSVTTNIMGRTICALGDAAS
                     MPVQSFVKHFGSEFEYHIENKKCLVPPEVQRAGSQIYVSPSC"
     gene            41089..43428
                     /gene="nuoG"
                     /locus_tag="PKN25_00805"
     CDS             41089..43428
                     /gene="nuoG"
                     /locus_tag="PKN25_00805"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNV89462.1"
                     /translation="MLEIEIDGKQVQVNDGSTVIEAAAVAGSYIPHFCYHKKLSIAAS
                     CRMCLVQVEKAPKPLPACATPVTNGMKVWTHSELAVKAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGSTASRYQEEKRVVFNKNLGALVSTDMTRCINCTRCVRFTTEIA
                     GEMELGQAFRGEHAEIMPFIEKTVDSELSGNIIDLCPVGALTSKPFRFAARTWELSRR
                     KSVSPHDSLGANLIVQTKHDVVKRVLPFENEDVNECWLSDKDRFSYEALSSEQRLGAP
                     MIRQGGQWKQVDWQAALEFVATGLRGVERDHGAASIGALASPHATVEELYLLQKLMRG
                     LGSENIDFRLREVDFRADGKRAGAPWLGMPIAGVKDLTRLLVVGSFLRKDAPLLAQRV
                     RQAAKKGLHVSAIGPNAEEWLLPVRNRALVAPSAMVATLGQVVVALAKEKGAEVSVAL
                     LGKLPETVSDEALAIAQSLASGRKVAVWLGNLAVQHARASELQMLAQEIARLTDGSFG
                     FIGEAANSVGGYLAGAVPGAGGLNAAAMIEQPRAAYVTLNLEPDLDLANPAAAMAALD
                     GAQLVVALTSFDSPSLRQVADVMLPIAPFTETSGTFVNCEGRAQSFTAVAKPFADVRP
                     GWKVLRVLGNLLALEGFAHADSEAVRAEALAGDLASRLDNGVEGIVVSSATGPAGVLE
                     RVADVPIHFADPIARRAPSLQKTRDAAAPTARIAPATLAALGLAAGDRVRVAQGTASV
                     ELVAQADDGLAAGCVRVAAAHPSTAALGAMSGDLSVERA"
     gene            43428..44477
                     /gene="nuoH"
                     /locus_tag="PKN25_00810"
     CDS             43428..44477
                     /gene="nuoH"
                     /locus_tag="PKN25_00810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNV89463.1"
                     /translation="MEAMLQPVADFFGPSWTAIWTLLKIVAIIAPLMICVAYLTLAER
                     KVIGYMQVRIGPNRVGPKGLLQPIADGMKLLFKEIIVPSGASKGLFILGPILAIAPSL
                     AAWAVVPFDDGLVLADVNAGLLFLLAITSMEVYGVIVAGWASNSKYPFLGSMRAAAQM
                     VSYEVSMGFALICVLLISASLNLTDIVMSQNEGRFADMGLGVLSWNWIPLFPMFVVYV
                     ISGIAETNRAPFDVVEGEAEVVAGHMVEYSGMTFALFFLAEYANMILVSILTSILFLG
                     GWLSPVGFLPDGFHWLAVKTAFILLLFLWARATFPRFRYDHIMRLGWKVFIPVTLVWL
                     FVVTVWMMSPLSIWS"
     gene            44485..44970
                     /gene="nuoI"
                     /locus_tag="PKN25_00815"
     CDS             44485..44970
                     /gene="nuoI"
                     /locus_tag="PKN25_00815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNV89464.1"
                     /translation="MGAKDYIGSLFLKELVKGMALTGRHFFQRKITILFPEEKTPQSN
                     RFRGLHALRRYPNGEERCIACKLCEAICPAMAITIESDQRDDGSRRTTRYDIDLTKCI
                     FCGFCEEACPVDAIVETRVLEYHGEQRGDLYYTKQMLLAVGDRHESQIAADRQQDAKY
                     R"
     gene            45004..45609
                     /locus_tag="PKN25_00820"
     CDS             45004..45609
                     /locus_tag="PKN25_00820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNV89465.1"
                     /translation="MEFKTFVFYFLAAIMVLAALRVITARNPVHAALFLVLTFFNAGG
                     LWLLLQAEFLAVTLVMVYVGAVMVLFLFVVMMLDINLDRLREGFWSYLPVGALVGILM
                     LVEMVMVLGGGYFGLEAMPAPAPVAADYSNTRELGRVLYTDYVYAFELASLVLLVAMV
                     SAVALTLRKRKGLKSIPPSEQVAVRREGRVELVKMQAEKDD"
     gene            45672..45977
                     /gene="nuoK"
                     /locus_tag="PKN25_00825"
     CDS             45672..45977
                     /gene="nuoK"
                     /locus_tag="PKN25_00825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNV89466.1"
                     /translation="MLSLSHFLILGAILFAISVVGIFLNRKNLIVLLMAIELMLLSVN
                     MNFVAFSHYLGDIAGQVFVFFILTVAAAESAIGLAILIVMFRNMRTIHVDDLDSLKG"
     gene            45990..48017
                     /gene="nuoL"
                     /locus_tag="PKN25_00830"
     CDS             45990..48017
                     /gene="nuoL"
                     /locus_tag="PKN25_00830"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNV89467.1"
                     /translation="MSDMKAIYLLVPLAPLAGAILAGLFGKQIGRAGAHVVTILGVAI
                     AFVASLFIYQDVAAGNTFNGTVYTWMQAGGIDFEVGFLIDSLTVMMMLVVTFVSLMVH
                     IYTIGYMSEDPGYQRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLLIGFWY
                     ERPTAIYANMKAFLVNRVGDFGFLLGIGLVAAYAGSLDYAEVFAKAKELSVTEMAVTG
                     WPLLTAICICLFIGAMGKSAQVPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVARM
                     SPLFELSDTALSFVLVIGATTALFMGFLGIVQNDIKRVVAYSTLSQLGYMTVALGVSA
                     YSAAVFHLMTHAFFKALLFLGAGSVIIGMHHDQDMRNMGGLRKYMPITWITSLLGSLA
                     LIGFPFFSGFYSKDSIIEAVHSSTIAGSGYAMFCVLLGVFITAFYSFRMYFLVFHGDE
                     RFGKAHDHAHHDHHGDHDDEEPSADHHHGLAPGQKPHESPWVVTLPLVLLAVPSVLIG
                     FFTIDPMLFGEWFKGVILVGDNHVGLRELAANYHGPVGMAIHGLQTAPFWLAMGGVAL
                     AWFFYMKRPDIPAAIQRTFKPIHTLLENKYYFDRFNEIFFAAGARLLGRGLWKAGDQA
                     LIDGVAVNGSARLVGWVAQMSRLFQTGHLYQYAFMMIIGVFVLLTYWFNRG"
     gene            48092..49573
                     /locus_tag="PKN25_00835"
     CDS             48092..49573
                     /locus_tag="PKN25_00835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNV89468.1"
                     /translation="MTDIPLLSLAIWVPIIGGLLVLATGSDRNAPLARMLALAVAVAG
                     FVVTIPLYTGFDVGTSAMQFVELQSWVPRFNINYHLGVDGISVLFVLLNAFITIIVVA
                     AGWQVIQTKVAQYMAAFLIMSGLMNGIFSALDAVLFYVFFEASLIPLYLIIGIWGGAN
                     RVYAAIKFFLYTLFGSLLMLIALLYLFMQSGGSFSILDWHQLPLPIEPQILIFLAFLI
                     AFGVKVPMWPVHTWLPDAHVEAPTGGSVVLAAIALKLGAYGFLRFSLPIVPDAANELA
                     PLVITLSLIAVVYIGFVALVQADMKKLVAYSSISHMGFVTLGFFMFNSLGVEGALVQM
                     ISHGFVSGAMFLCIGVLYDRLHSRQIADYGGVVHTMPKFAAFFMLFTMANSGLPATSG
                     FVGEFMVVLGAVQYNFWIGFVAATTLILGAAYSLWMYKRVVFGKVANPHVAELEDING
                     REFAFLAVLAACVLAMGLYPFPFTEVMHASVNELLRHVAVSKL"
     gene            49622..51091
                     /gene="nuoN"
                     /locus_tag="PKN25_00840"
     CDS             49622..51091
                     /gene="nuoN"
                     /locus_tag="PKN25_00840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HNV89469.1"
                     /translation="MNFVVPDFYPAAAEIFVAVMALVIMLASTFARSVARNLAYLLTQ
                     ATLIAAAFITIFTMEGEVVHTFSNLFISDLLGDVLKLMIYFSTAIALLYGRGYLADRK
                     IDKPEYYLLALLMTLGMMVMVTANHMLPMYIGLEMMSLALYTMVAFDRDSARSTEAAM
                     KYFVLGALASGLLLYGMSMVYGATGTLEFSGVAQALYNQTANQTVLMFGVVFLVAGIC
                     FKLGVVPFHMWVPDVYQGAPTAVTLVIATAPKLAAFAMAVRLLIWALFDIAEEWQMML
                     MLVAAASIVLGNLAAIAQQNIKRMLAYSGISHMGFMLLGLLAGVVEGDRHFALNAYSS
                     AMFYAISYVIMSLASFGMLILLSRAGFEAENIDDFKGLNKRSSWYALMMLFVMFSMAG
                     VPFFIGFFAKLSVLQAVVAAGYFWLAVLAVVMSVIGAFYYLRVVKVMYFDEPVDSSPI
                     HAAPEVRVMLSANGLAIAALGLAPQALMSLCAFALLRSL"
     gene            51099..51431
                     /locus_tag="PKN25_00845"
     CDS             51099..51431
                     /locus_tag="PKN25_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HNV89470.1"
                     /translation="MGADASVFLLLAIAFTMANLPFVFDRIFLIRKPPAGVRKGLGWR
                     FAELLVLYFVVGGLAASIEAQSHGRVYGQGWAFYATTFCLFVVFAFPGFTWAYLWRSA
                     RRTSEVGA"
     gene            51428..51997
                     /locus_tag="PKN25_00850"
     CDS             51428..51997
                     /locus_tag="PKN25_00850"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNV89471.1"
                     /translation="MSGLERDALHEYTLDSESVFDGALLKVWRDRVELPDGSRSVREY
                     IRHPGAVVVVALEESGALVFERQFRYPLQRAFLELPAGKIDAGEDILACAQRELREET
                     GFAAANWEYVGVMHPCIGYSDERIEVFFAHELSQVGGALDDGEFLEVLSLRADEVEQA
                     IRIGRITDAKTITAYYLALPHIQARLRGR"
     gene            complement(52029..53309)
                     /locus_tag="PKN25_00855"
     CDS             complement(52029..53309)
                     /locus_tag="PKN25_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV89472.1"
                     /translation="MSQPDGFHFIPRIARIDARQWDALVDASCASAAPCIRHAFLHAL
                     EESGCVGGRSGWTPAHATLWRGGTLGAAMPLYVKTHSRGEYVFDWAWAEAYQRHGLDY
                     YPKWLAAVPFTPIPGPRLLGHDDGARRELLAALLAKALESGLSSLHLLFPLDRERPLL
                     EAAGLMIREDVQFHWRNPPASAETEPTPAESSAPISVRQRRWADFEHFLASLSGAKRK
                     KIRQERRRAAAHGLDLQWLDGHEARDEDWAFFAHCYANTYAQHRSTPYLAADFFVRLA
                     HSMPEAVRLLVARRDTQPIAAAFFLCDREALYGRYWGCVEDLPFLHFELCYYQAIEYC
                     IEHGLTRFEGGAQGEHKLARGLLPVRTFSAHWLADPRFRGAVDDWLARERAGVGHYLD
                     ELAEHSPFLREKGPGGIETGAAGAAEPQRGSRRQ"
     gene            53606..55234
                     /locus_tag="PKN25_00860"
     CDS             53606..55234
                     /locus_tag="PKN25_00860"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNV89473.1"
                     /translation="MNAHAQAPVSIAVAQLNLTVGDLVGNADHIIAALDDARAAGAQL
                     LLTPELALSGYPPEDLLLRPDFYRACAREVERIAAAARGVTVVLGHPVEDGGERYNAA
                     SVLRDGAVLTRYHKSLLPNYEVFDEERYFEAGGTACVFECAGVRFGVNICADVWERGP
                     AEAARAAGAQVLLALNASPYHMNKQAQRLEVLRARVAETGLPVLYCNMVGGQDELVFD
                     GASFALDAGGAVGLQLGSFVERVAIVDYASGGWSGGEHVPARTLEAEVYEALRIGVRD
                     YLAKNRFPGAIIGLSGGIDSALTLAIAVDALGADKVRAVMMPSPYTAQMSLDDSRDMV
                     ARLGVRYDEIAIEPAMKVFAELLADQFAGLPADTTEENLQARIRGMLLMALSNKTGAI
                     VLTTGNKSEMATGYATLYGDMAGGFAVLKDLYKTFVFRLSNWRNTVSPVIPQNIIDRP
                     PSAELKPDQKDQDSLPPYDMLDAIIQAYMERDESPRDIVAAGFPEGEVRRVVGMLKRN
                     EYKRRQAPVGIRVTQRGFGRDWRYPITSRYQDEF"
     gene            55251..55589
                     /locus_tag="PKN25_00865"
     CDS             55251..55589
                     /locus_tag="PKN25_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNV89474.1"
                     /translation="MKKIEAIIKPFKLDEVREGLSEVGITGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIKVEVVLADDIVDQAVEAIVKAAHTGKIGDGKIFITPVEQVIRI
                     RTGETNEAAI"
     gene            complement(55607..56713)
                     /locus_tag="PKN25_00870"
     CDS             complement(55607..56713)
                     /locus_tag="PKN25_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNV89475.1"
                     /translation="MSRRAPAPGPAGNPGRDAQGAVPKPASPFAALRKQLQQRALPAP
                     VSSPAPARRIRHPLEEDSSSAGADRAQEPDAEALELFRRSVGAVRPVRGTDRVEIHRP
                     RPAPRPRTQAVEDEETEEPVRARPETDPLRAAYEGVMPLRDTGRVALDTPLRHHARHA
                     GGTHPAPALRPDAIVLPADADVSDPAALFLAVVGNARPVTDRNRVELERPQPAPAPLK
                     REEDERAALGESLAAPLTFEDRLDMGDEAAFLRTGLPRRVLTDLRRGRWVLQGQIDLH
                     GLTRDEARAALANFLHDALAQGKRCIRVIHGKGHGSPGKVSILKQLSRGWLAQREEIL
                     AFCQAGPHDGGGGALLVLLRAQNAAPRARMPLPA"
     gene            complement(56722..57675)
                     /gene="trxB"
                     /locus_tag="PKN25_00875"
     CDS             complement(56722..57675)
                     /gene="trxB"
                     /locus_tag="PKN25_00875"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNV89476.1"
                     /translation="MTTKHARLLILGSGPAGYTAAVYAARANLNPVLITGLAQGGQLM
                     TTTEVDNWPADADGVMGPDLMARFQRHAERFNTEMIFDHIHTVKLGEKPFRLIGDSGE
                     YTCDALIISTGATAKYLGLPSEEKFAGRGVSACATCDGFFYRNQDVAVIGGGNTAVEE
                     ALYLANIARKVTLVHRREKFRAEKIMIDKLMEKVAAGRIELALNATLDEVLGDNTGVT
                     GMRIKGVNNGATREVALQGVFIAIGHKPNTDIFEGQLEMEGGYIVTQGGRNGNATQTS
                     VPGVFAAGDVQDHIYRQAVTSAGTGCMAALDAERYLDALGV"
     gene            57945..58616
                     /locus_tag="PKN25_00880"
     CDS             57945..58616
                     /locus_tag="PKN25_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV89477.1"
                     /translation="MTQATAVSVVALKTFPLFHGLSDESLASIARVSMMRRFPRGQSV
                     VCAGDRPDYVYLVLTGSLKVVVSDEDGREVILSILGQGELFGEMGMFDERPRSASVIA
                     VVPADLVLISKQDFRRIMQDSFDVSWRIMCNLAERLRNADRKIESLALMDVYGRVARL
                     LIEMAEDVGGQSVVVRRISKQDIAKMIGASREMVSRVMKDLGQQGLIEEGPDGILLRE
                     RLNDV"
     gene            58727..>60611
                     /locus_tag="PKN25_00885"
     CDS             58727..>60611
                     /locus_tag="PKN25_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNV89478.1"
                     /translation="MLSRSSSRSQPLPEKISLLLQEARWLILGVVSLYVGMILVGYSE
                     SDPGWSHASDVTRVANPGGRFGAWLADLLLYLFGISSWWWVVFLGYSLLWGFRRLKNR
                     LELDHRSFIFVMIGFFVVLITSSALEFLRFHSHGAALPLEPGGLIGMELAQFVRRSFG
                     FTGGTLLLLALLATGLSLFTGVSWLALMERIGLGIEQAVLGVQQAWLRWQDRRVGREV
                     AHKREEVVQTRRKKVEKAEPAPLRIEPAVVAVPKSERVEKERQQTLFQDVPEGVIPPV
                     ALLDPASGGVEPPSPESLEFTSRLIETKLADFGVEVKVLAAYPGPVITRYEIEPATGV
                     KGSQVVNLAKDLARALSLVSIRVVETVPGKSCMALELPNPKRQMVRLSEIIGSKVYQD
                     AHSPLTVVLGKDIGGQPVVADLAKMPHLLVAGTTGSGKSVGINAMILSLLYKSEPDRV
                     RLIMVDPKMLELSIYEGIPHLLAPVVTDMKHAGNALNWCVAEMDKRYKLMAAVGVRNL
                     AGFNKAVQEARKAEQPLTNPFSISPENPEPLEPLPYIVVVVDELADMMMVVGKKVEEL
                     IARLAQKARAAGIHLILATQRPSVDVITGLIKANVPTRIAFQVSSKIDSRTILDQMGA
                     ETLL"
BASE COUNT         9400 a        19781 c        20817 g        10413 t
ORIGIN      
        1 gggcgcagga ctatgcgatg gccaaccgcc aggcgatgct cgagctcgtg ctcgcagggc
       61 tggcgcgcca cctgccgccc tttaccgtga ctaccgaggc ggtgaactgc caccacaact
      121 acgtcgcccg cgagcaccac ttcggcgccg acgtgtgggt gaccagaaag ggcgcgatcc
      181 gcgcggggga gggcgagctc ggcatcgtcc ccggcagcat gggcgcgcgc agctacatcg
      241 tgcgcggcag ggggaacgcg gagagcttct gttccagtgc gcacggcgct ggccggcgta
      301 tgagccgcac ggcggcgacc aagcgcttca ccgaggccga cctcgcgcgc cagaccaagg
      361 gcgtgatctg ccgcaaggac aagggggtcg tcgacgagat ccccggcgcg tacaaggaca
      421 tcgacaccgt gatggccaac cagtcggacc tgaccgaggt gctgcacacg ctgaagcagg
      481 tggtgtgcgt gaaagggtag ggggcgcaac agatggcagg ggaacgggtt cgaactgtca
      541 tggaccacgg ctggccgtgc tgtgcgaaag acaggctcac ttcctgagcc tgtcgcgcat
      601 tacagtcacc gcactacatc cagcacggag tgctcatgcg gcctgcccga tgcgcagcgg
      661 cacaaaagct cctggcgcga gcggaaggag cggtacctcg ctcacctgcg tgcggcggaa
      721 ggcgagggca ttctcgtctc tgcgtgcgac aagctggcca acctgcaggc gatcctgttt
      781 gatctgactg aaatcggtga gtccgtttgg gcgcgcttca gcggagggaa gggtggatcg
      841 ctgtggtact gcacggcgtt agtcgatgct tttggcggac gggtaccggc cgcaatggga
      901 tgtcgacgat tggctacgca caactcatca gccgactctg tctggtcgtc cgaccgctgg
      961 cgaagccggc gcagatcagc ggttcggtca atcggcctgt ggactcggcc gaccgcgtgc
     1021 ttcttccttg cggcgttgcc atcgagggca cgctggtcgg gcacctcgag tttgccctgc
     1081 gccatgaagg cgtgaatctt gacgtcatcg acgccctgtt cgagcacctc acgccggagg
     1141 acttgctggc caggttgaac gcggcgccca acggcgcgca catccggcgg gcgtgccatc
     1201 tctgggagtg gctgacggag ccgagaactg caggcagctg ccttgccgat gggaggctac
     1261 gtcgacctgt ttcctgcccg atgactacgt caccgccggg cagcccacca acgaccgtcg
     1321 attccgcgtg cgcaaccatg cactggggac cgccggtttc tccccggtgg tgcggtgcgc
     1381 ggcagtaacg agcgcgcgcg cgatgagggg ctgggaggcc ttcatcaacg acgaccaccc
     1441 ctgcccggat atcaccgcgc atcccggaag ccgggcctgc cgattggacg tcggctgtgc
     1501 gcttcggcgg tcgggcaggg cccggcccct gcggcgcatg gagcggagcg cgccgaaaag
     1561 gccccgcatg gccgttggat tgggctctca tgtttgnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnngctc tcatgtttgc atcgataaat ctgatgattt ccattgacac
     1741 gccttccggg ctcccttagt ctggtgcaac ttctggctaa gtggtaagac cacttagcca
     1801 gaaagtggaa gcgcacgaca tcctgatttc atgaaatgca ttcgagaggg gaaggggcaa
     1861 tgacggttgg ttcgatgttc aaggctgcgc gcgtgatgcg cgcgccggcc tgagggggag
     1921 attccgatgc agcctggtac gctcgcattc ctcgcctttt ctccgatcct gctcgccgcg
     1981 gtgctgctgg tgggcctgcg ctggcccgcc aagcgcgcga tgccggtcgt ctatttcctc
     2041 actgcgggca tcggcctgta tgtgtgggag atgagcctga accgcgtgct cgcctcgacc
     2101 ctgcaggggc tggtgatcac gatcggggtg ctgtggatca tcttcggcgc catcctgctg
     2161 ctcaacaccc tgaagtactc gggcgggatc accgcgatcc gcgcgggctt cgccaagatc
     2221 agccccgacc gccgcatcca ggcgatcatc atcggctggc tgttcggctg cttcatcgag
     2281 ggcgcctcgg gcttcggcac gccggcggcg atcgccgcgc cgctgctggt ggcgatcggc
     2341 ttcccggcgc tggcggcggt gctgctcggc atgctggtgc agagcacgcc ggtgtccttc
     2401 ggtgccgtgg gcacgccgat catcatcggc gtcaatagcg gcctcgacag tgcgacgatc
     2461 ggcgcgcagc tcgccgccag gggctcgagc tgggacgtct tcctgcaggt gatcaccagc
     2521 aacgtggcga tcatccatgc catggtcggc accgtcattc cgctgatcat ggtcatgatg
     2581 ctgacgcgct tcttcggccg cgagaagagc tggaaggctg gcttcgaggt gctgcccttc
     2641 gcgatcttcg cgggtctggc cttcaccgtc ccgtacgcct tcaccggcgt cttcctgggg
     2701 cctgagttcc cctcgctgct cggcggcctg gtcggcctgg ccctcgtcac caccgcggcg
     2761 cgtttcggtt tcctggtgcc gaagaagacc tgggacttcg ccgacccgaa ggagtggccg
     2821 agcgagtgga tgggcagcgt cgagatgaag ctcgatcagc tcccgcgtca ccagccgatc
     2881 agcatcttcc gcgcctggct gccctatgtg ctggtgggcg cgctgctcgt gctgagccgc
     2941 gtgttccccg agatcggcgg ggcgatgaag tcggtggtga tcgtgttcgc cgacatcctc
     3001 ggcgagaagg gcatcaaggc ggacttccag ccgctctacc tgcccggcgg catcctggtg
     3061 gcggtggtgc tgatcaccgt gttcctgcat ggcatgcacg cgcgctcgct ggtgaaggcg
     3121 gtgggcgagt cctcgcgcgt gctgctcggc gccggcttcg tgctgctgtt caccgtgccg
     3181 atggtgcgca tcctgatcaa ctcgggggtg aacgccgccg acctgcccag catgccgatc
     3241 gcgatggcca agtgggtggc cgacagcgtg ggcaacgtgt atccgctgct ggcaccgtcg
     3301 gtgggcgcgc tcggtgcctt catcgccggc tcgaacacgg tcagcaacat gatgctgagc
     3361 cagttccagt tcggcgtggc gacctcgctc ggcatctcca cggcgatggt cgtggcggtg
     3421 caggcgatcg gtgcggccgc cggcaacatg gtggcgatcc acaacgtcgt ggcggcctcg
     3481 gccacggtgg gtctgctcgg ccgcgagggc gcgatcctgc gcaagacctt ctggccgacg
     3541 gtgtactacg tgctgatgac cggcacggtg gcgatgatcg cgatctacgt cctcggggtg
     3601 tccgatccgc tgctcgccgc gagctgagtc ccgcgcggag ggacgcatcg gcgtcccccg
     3661 cgcatccgtc cttcctgccg tcgcggcccg gccgcggctg catcgcactt cagaacgatg
     3721 aaattccgga gccgaagcgc atgagtcctc tgtcctccgc acccgccaac gccacacgtg
     3781 tcgcgccgcc gctcgaggcc gcgcgcagct atccggccaa gccggccgac gtgtacctgt
     3841 tcggcacctg cgtgatcgac gtgttctttc ccggtgccgg gatggacgcg atccgcctgc
     3901 tcgaacgcga gggcatccgg gtgcattttc cgcaggcgca gagctgctgc gggcagccgg
     3961 cctacacctc gggttatacc gacgaggcgc gcgaggtggc gcgcgcgcag ctcgcgctct
     4021 tcgccggcga ctggccggtg gtgatcccgt cgggctcgtg cgccggcatg ttccgccatc
     4081 actacttcga gctcttcaag gacgagcccg agacgctgca gcaggtcgcg tcgctgtccg
     4141 cgcgcacctt cgagctcgcc gagttcctgc tggaggtgtg caaggtccgc ttcgagcaca
     4201 ccggcgcgcc ggtcaggctc gcgttgcaca cgtcctgttc ggcgcgacgc gagatgaaca
     4261 cccacctgca tggccgcgcg ctgctcgcgc agctggcggg ggtcgagcgc gtcgagcacg
     4321 accacgagag cgagtgctgc ggcttcggcg gcagcttcag cgtgcgcatg cccgagatct
     4381 cgggcgccat ggtgcaggac aagaccgccg cgctgaaggg ttcgggcgcg gccgaggtgg
     4441 tgagcgcgga ctgtggctgc ctgctcaaca tcaacggctg cttcgagaag cagggcgaca
     4501 cgcttcgcgg cgagcacctg gcgagcttcc tgctgcgccg cacgacttcc gcgagcacgg
     4561 gaggggtgcg atgagccacg tcgccgacac ccccgcgcag ccgatcgcct tcacccgcaa
     4621 cgcgcgcgag gcgctcgccg acgcgcagct gcggcgcaac ttccgcggcg ccatggatgc
     4681 gctgatggac aagcgccgca accagtttcc cgacggcgac gagctcgagc gcctgcgcgc
     4741 cttcggcaac cgcgtgcgcg cgcgcgcgct gtcgaagctg cccgatctgc tcgagcgcct
     4801 cgagtccaat ctcgtccgca atggtgtgca ggtgcactgg gcggagacgg tggaggaggc
     4861 caacgccatc gtgcatggca tcgccgagcg ccacgcggcg aggaaggtga tcaagggcaa
     4921 gtccatggtg tccgaggaaa tggagatgaa ccattacctc ggtgcgcgcg gcgtcgactg
     4981 cctcgaatcc gacatgggcg agttcatcgt gcagctgcgc gcggagaagc cctcgcacat
     5041 catcatgccg gcgatccacc tcaacgccgg ccaggtcgcg aagctgttcc acgacaagct
     5101 cgcggtcgag tacaccgagg acgtcgatcg cctcatccag atcggccgcg agaccttgcg
     5161 gcgcaacttc ttcgaggccg acatcggcgt ctcgggggtg aacttcgcga tcgccgagac
     5221 cggcacgctg ctgctggtgg aaaacgaggg caacggccgc atgtcgacca cggtgccgcc
     5281 ggtgcacatc gccgtcaccg gcatcgagaa ggtggtcgag aacctgcgcg acgtgatccc
     5341 gctgctgtcg ctgctgacgc gctcggccac cggccagccg atcaccacct acgtcaacat
     5401 gatctccggc ccgcgcaagg ccgacgagct cgacggcccg caggaagtcc acctggtcct
     5461 gctcgacaac ggccgcagcc aggccttcgc cgacggcgag ctgcgccaga cgctcaactg
     5521 catccgctgc ggcgcgtgca tgaaccactg cccggtgtat acgcggatcg gcggccacgc
     5581 gtacggcacg gtgtatcccg ggccgatcgg caagatcgtc accccgcaca tgctcggcct
     5641 ggagaccacc cgcgacctgc ccacggcctc gtcgatgtgc ggcgcctgcg gcgaggtgtg
     5701 cccggtgaag atccccatcc cgtccctgct gcgccgcctg cgcgaggagg cggtgcgtgc
     5761 gcccgcggcc gagccgcatc acgtgcgcgg gcagggcgcc aagcactcca ggaaggagac
     5821 cctggtgtgg agggcgtggc gcacgctcaa cacctctccg ggcctgtacc gcgcggcgat
     5881 gtacgcgggc acccgcctgc gcgggctgat gccggccgac atcgggccgt ggacgcgcta
     5941 ccgcagcgcg ccgaaaccgg ccgcgcgcag cctgcacgag ctcgcccgcg agcatctggg
     6001 cgagcaatga acgacgccag gagcgaaccc cgaatgaatg cgaaagaacg gatcctcggc
     6061 aagctgcgcg ccagcctcgc ggggacgacc ccggtcgccg acgactacga catcggcctg
     6121 gtcaccgatc cctggcgcta tgcggcggac gagcgcatcg cgcgcctgcg ccgcatgctc
     6181 gaggcggtgc atggcgagac gctcgtcacc acggtggcgc agtggccgca gcgcgtgctg
     6241 gaggcgctcg ccgcgcgcgg catcgacgag ctgctggttt cgcctgccac cgcgcacggc
     6301 cgccgcctgg cggagcacgt gcgtgcacag gatgcggcgc tgcgcctgaa gggctacgag
     6361 aacccggtgg aggcctggaa ggacgagctc ttcttccata cgcccgccag ccttaccggc
     6421 acgctcggcg gcatcgccgc caccggcagc ctggtgctgt ggccgagccc gcaggagccg
     6481 cgcctgatga gcctggtgcc gccgctgcac atcgcgctgc tgaacgcgag cgaggtgcgc
     6541 gacaacctct tcgagctcat gcacgcgcag cgctggaccg acggcctgcc gaccaacgcg
     6601 ctgttggtgt cggggccgtc caagacggcc gacatccagc agacgctggc gtacggtgcg
     6661 cacgggccga aggagctgct ggtgctggtg atcgaggacg cctgagcgcg gcgcggatcg
     6721 tgggcgcgcc cggccgtgcc gacgatcggc gccgcggctt gccggagcgg cggaatcgcg
     6781 actcgcgtcg ctcctaccgc gcggctcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnccc gcacccgcag cttcttctgt aggagcgacg caagtcgcga tcgcggcgac
     6961 cgcctaccgg cgccgcgtcg cctcatgaat cgctgctcgc gcctccgtcg actcgcctca
     7021 cccctccgat ggtggtcgga gctcgaagct cgcccgcacc acccccggct cctccgaggc
     7081 cttgagcttg aagcccaggt gcttcagcag gccgagcatc ggttcgttct cgcccatcac
     7141 ctcgcccacg aggcgctgga tgccgatctc gcgcgcgtag cgcaccagct tgtccatgag
     7201 gatcgagccc aggcgctggc gcttgaggtc ggaacggatg acgagggcga actcggcggt
     7261 gtggttgtcc ggatcggcca ccgcgcgcga aacgcccagg gtctcgggct tgccgtcggg
     7321 gccggtcgcg gtggcgatga aggccatctc gcggtcgtag tcgatctgcg tcaggcgcgc
     7381 catctcgcga cgcgggaact cggggatgta gttgaagaag cgatagatga agtcctcgcg
     7441 ggtgagccgc gacaggaagg cgtagtgcgc cggctcgtcc tcggggcgga tcgggcgcag
     7501 caggatcggg cggccgtcgc gcagccgaga gggctcctcg agcgccttcg ggtagggctg
     7561 gatcgccagc cggtgctcgt gggcggtggc cggacgcagg cgcatgtggg cgtcgagcgc
     7621 ctgcacgccg tgctcgtcga cgaacaccgg gttgatgtcc atctcgtgga tctcgggatg
     7681 gtcgacgacg atctgcgaga ccttggtgag ggtgagcgcg agcgcgtcgc ggtcggccgc
     7741 ggggaggtgg ccgcgcgcct cgagcagcgc tgccgccttg ctgcgggtga tgaggtcgtc
     7801 ggcgagcggc ttgttgagcg gcggcaggcc gaccgcgagg ccacggaaga tctccagcgc
     7861 gcgaccgcct tcgccgaaca cgatcaccgg tccgaacagc cggtcggtgc tgacgccgat
     7921 cagcagctgg cgcgcgtgcc cgcgcatcac catgcgctgc acggtgaagc cgctcaccga
     7981 caccggcggc tcggcgcggc cggcgcgctc gatcatgccg cgtgcagcga cctggaccgc
     8041 ctcggtgctc tcgaggttga gcgcgacgcc accgacgtcc cacttgcgcc ggatgtcgcg
     8101 cgacatcagg gtcaaggcca ccggcaggcc gatgcggccg gcgatctgcg cggcctcttc
     8161 gggggtggcg gcgaggtggg tctggatgac gggcacgcca taggccgcga ggacgtcctt
     8221 ggattcggac tcggtcaggc tggcacgcga ttgggcgagc gcgccctgga tcaccgcccg
     8281 cgcgcgcgcg gcgtcgggct tgaaggcttc gggttcggag gtcggcacct ccatcagcac
     8341 ctcggaggcg atgcggtggc ggtgcatgtg caggaaggcg ctggccgcgt gctcgggcga
     8401 gtcgaaggtg gcgatgccgg cgttgatgaa gaggttgcgc tctttctcca cgctctcgtc
     8461 gcccacccag caggtcatca ggttgatgcc ggtctcgcgt gcggtggcga tgacctgctg
     8521 cgcggtgtcg atgctgctgg agagcgcgtt cggggtgtgg atcagcagca cggcgtcgcc
     8581 gtcgcgctct tcctgcagga tcttgagcag gcgctcgtag caggccggct cggagtcgac
     8641 gcggatctcg agcggattgc gtccgttcca ggccggcgac atgaagcggc gcaggcgctc
     8701 gatggtctgc tccttgagcg ccggcaggct gtagccggca aggtagaggc tgtcctccac
     8761 catcaggccg gcaccgccgc cgttgctcag cacgaccagg cgattgccgc gcagcgggcg
     8821 ggcgcgcagc acggtctcgg cggcgccgaa catttcttcg agctgatcca cccgcaggat
     8881 gccggcgcgg cgcagcgccg cgtcgtgcac gtcgtcgtgg ctgaccaggt tgggcatctc
     8941 gaggaagagc gggtcggaga cgcccatcag cggattgtcg ggcgagcgtc cggccttgat
     9001 cgcgatcacc ggtttgttgc gcgcggcggc gcgcgcggcc gacatgaagg cgcggcgatc
     9061 ctggatggat tcgatgtaga gcaggatcgc gcgcgtgccc gagtcggcgg cgaggaagtc
     9121 gagcacctcg ccgaagccga tgtccaggct gtcgccgagc gagacgaagt gcgagaagcc
     9181 gaccttcttg cgcagcgccc agtcgagcac catggtgccg accgtgtcgc gctgggagac
     9241 gaagccgagc ttgccggggc gcgcggcgat ctgcgagaag gtcgcgttga gcccgagtcc
     9301 gggtaccagg atgcccagcg tgccgccacc gagcaggcgc atctcgtagc ggcgggtgat
     9361 ctcgaggatg ctgcgctcga tcggacggcc gtcggcgccg agcgtggtgt cgagctggtt
     9421 ggccatcacc atcacggcac gcgcgccgcg ttcgccgagc tcctccacca ggcggggcac
     9481 gagcggcgcg ggggtgcaga tgatcgccag gtcgggcgtc tgcggcagcg cggcgatgct
     9541 cgggtaggtg agcacgcccg ccaccgaggt gcggcgggga ttgaccggca tgatcgggcc
     9601 ggcgaactgg cccgaaagca ggttgcgcat gagcacgttg cccatgcggc gctcgcgcgc
     9661 gctcgcgccg atcacggcga tcgagcgtgg attgaacagg cttttcaggt ggcggaagct
     9721 catcgccttg ctccccgggg aggaatcgct cgtctgcagt ttaatgcatg cctgccgcac
     9781 atgcatgaac tgaattgcgg ccgggtcgag cggcccgcca gcgcccggtt ctccgcgtcg
     9841 aactcgagct gtccggacga ggttcagccg gcggcgacgg tgtgcatcag atagtttacg
     9901 tccgtgtcgc ggccgagcga gtaggtcttg gcgagcgggt tgtaggacag gccgatgatc
     9961 tcctgcacgt ccaggtgcgc attgcggcaa tggcggctga gttcggaggg cttgatgaag
    10021 cgggcgtact cgtgggtgcc ggccgggagc aacttgagca ggtattcggc gccgacgatc
    10081 gcgaacaggt aggacttcgg attgcggttg agcgtggaca ggaagacgtg gccgccgggt
    10141 tttaccagcc gggcgcaagc ggtgacgatg ctggccggat cggggacgtg ctcgagcatt
    10201 tccatgcagg tgacgacgtc gaagcggccg ggcgattcct cggcgagcgc ctctgcgctc
    10261 atgtggcggt agtcgacctt gtggccgctc tcgaaaaggt gcaggcgggc gacgctgagc
    10321 gccttctcgg ataggtcgat gccggtcacc tcggcgccga gcgcggccat gccctcggcg
    10381 aggatgccgc cgccgcagcc gatgtcgagc accttcttgc cggcgagcgc ggccttgccg
    10441 tcgatccagc ccaggcgcag cgggttgatc tcgtgcagcg gacggaactc ggaggcgggg
    10501 tcccaccagc ggtgggcgag gtcgctgaat ttctgcagtt cggcgggatc ggcgttgacg
    10561 gtgttcatgg cgttcgaggg tccttgcttg cacgcacggg caccgggtgc gcgcgcgccc
    10621 ggcacaaacg aaaaagcccc gccgaggcag ggctttcggg ttaccgactc cggaattact
    10681 tggagccgat gacttccact tcgacgcgac gatccggctg caggcaggag atctgcgcgg
    10741 cgcggccacc gaccttgtcg cacttgttgc cggtcacggg ctgggtttcg cccttgcctt
    10801 cggtgtagac gcggttggca tcgacgccct tcgagaccag gtaggtcttg acggcggcgg
    10861 cgcgacgctc ggacaggcgc tggttgtagg tgttggaacc caggcggtcg gtgtggccga
    10921 cggcgaggat cacttccagc ttgagcgact tggcctgggc ggcgagttgg tcgagcttgg
    10981 ccttgccttc agccttcagg acggccttgt cgaagtcgaa cagggcgtcg gccgacagct
    11041 tgaccttgtc ggcggtgggc ttcggacccg gagccgcggc agcagccttc acggtgcact
    11101 tgtcggcggg gacgatgtcc ttgtcgcaag cgcagccgac cggcaggtcg ccggcgaggg
    11161 cggtcgaggc ggcggcgggg ctccagtagc cggtgcgcca gcacaggccg gtgccgctgc
    11221 gagcgacgac gttgcgcgcg tcgatcacgt aggggatttc acccttgccg gcgacgacga
    11281 cgtcgctgac ctgagcgaaa gcgctcgggg cggacaggcc gatcgaggcg atggcggcca
    11341 gcatgagcat ctgcttcttg gattgtttga tcatggtttc ctctttgaag ggcgagtcgc
    11401 cggaattgcc actgaacgaa atgaagactg gcgaatctgc cgtcttcgtg tcggtttgta
    11461 ccagtatatc cagttcaatt accgagctta gtttgccaca tcgacacgag ccggagcaat
    11521 tcggtgtcgt taatatgcaa cagcgagacg cctttgtcca cgcgggtttc gccgtcgatc
    11581 catacgtggg agacgttttc gcgaccgcat acgtagacga gatgcgacac cggatcgaaa
    11641 cagggccggc attgcggtgc ggaaaggtcg agtgcgcaaa gatcggcgca tttgcctttt
    11701 tcgatcgagc cgatgcgatc gtccatgccc aatgcgcgcg cgcccccgag cgtggccatg
    11761 cgcaaggcgg catgcgccgg taccgcggtg gcatccaggc tgccgacctt ggcgagcagc
    11821 gcggcgtggc gcatctcctg gagcaggtcg aggcggttgt tgctcgccgc gccgtccgtg
    11881 ccgaggccga ccgggacgcc ggcggcgagc aggcgcggca ccggcgcgat gccgctggcg
    11941 agcttcatgt tcgaggtggg gcagtgcgcg acgctgcagc cgtggcgggc gagcagttcg
    12001 atgtcggcct cgtccagatg caccgcatgg acgccgatca gattgctgcc gagcacgccg
    12061 aggcgcgcga ggcgagcgag cggccggcag ccgtggacgg cgagggagtc ggcgatctct
    12121 tgtgcggtct cgtggatgtg cacatggatc ggcaggccga gttcgtccgc gagggtctgc
    12181 acctggtgga agctgtcgtc cgagacggtg tagggcgcgt gcggggcgat cgagaagccg
    12241 agccgcgggt ggccctgcca gcggtcgcgg gcggccaggc ccttgcgcag gtagtcctcg
    12301 gggtcgctcg cgtacgaggt ggggaattcc agcacgaccg cgccgaccac cgcgcgcatg
    12361 cccgcggcgg cgaaggcttc cgccgccgcc tcggggtgga agtacatgtc gctgcaggtg
    12421 gtgatgccgc cacggatcat ctcggccgcg gcgagcaggg tgccgtcgcg cacgaaggcg
    12481 gcggaaacgt gacggctctc ggccggccag atcgcctcct ccagccagcg catcagcggc
    12541 aggtcgtcgg cgatgccgcg cagcaggctc atcgcggcgt gggtgtgcag gttcaccagg
    12601 ccggggatca gcaggtgccg cggcagctcg accacctcgt ccgcgacgta gcgcgtgcgc
    12661 gcctcgtcct ggccgaggag gtcgacgatg cgtccgccgc ggatcgccac cgcgtggtcg
    12721 gcgagcacag cgtcgcgcgg ttcgacgggg acgatccagc gggcgttgat cagcaggtct
    12781 acggcttcgc tcaaggcggc tcctggcggg cagttgcggg gagagcctca ttcaagcatg
    12841 cgcggcccgg gcggacaatc ccgcgcgtcg ctgcgggtgt ggaggcgcac gccggcaccg
    12901 gatcgtgcaa caatcgcgcc gaagcccgcc ggccgcgcgg gcgcccccgc cctgcaagac
    12961 agcatgaacc ggattcccga acgcatcggc aagtacagga tcatcaagga gatcggccgc
    13021 ggcgcgaccg cggtggtcta tctcgccgag accccggact accccgagcc ggtggcgctc
    13081 aagcacgtgc gcttcgacga caaggcgcgc gacgaggcga agtggaaccg ccgcctgatc
    13141 aagctgctga aggccgagcg cgtggtgtcg ggccgcctcg accaccccaa catcatccgt
    13201 atccacgatg cctcgatcga gcccgacgag gcctacgtgg tgatggagta cttcccgggc
    13261 ggctcgctgg agcgctactg cagcttccag aacctgctgc ccatccatcg cacgatcggg
    13321 atcgtgttca agtgctgcat ggcgctcgac cacgcttacc gccagggcat cgtgcaccgc
    13381 gacatcaagc cggcgaacat cctggtcgac gagcaggaca acgtgaagat caccgacttc
    13441 ggcctcgcgc tcaacatcaa caagaagctc gacaccgact cgaccttcat catgggcgta
    13501 ggctccccgg cctacatgag cccggagcag atcaagggct atccgctcaa ccagaagacc
    13561 gacctgtatt cgctcggcgt gctgctgttc cacatgctga ccgggcggct gcccttccgc
    13621 gcgagcaatc cggcgcagct cgtctataag atcatcaacg ccgatccgcc ctcggtctcg
    13681 cagctcaacc cggacgtgcc cgagcagatg gacgcggtga tccgcaaggc gctcgagaag
    13741 gacctctact cgcgctaccg caacggcgcg gagttcgcca aggacctcgc cgcggtccgc
    13801 taccagatcc tcgacgaagg tcacgtgcac cacgacgccg agcgcttcgc gcgcctgcgc
    13861 aaactggcct tcttcaccga gttcgaggac gtggatctgt gggaggtgat gcggatctgc
    13921 gcctggcgca aggtcggcag cgacctcggc ctgatgcgcg agggcgacct cgatcagcgc
    13981 ttcgggatcg tgctcgaggg caaggtcgag ctctccatcg gcgggcggcg ggtgctggag
    14041 gtggggccgg gcgaggtttt cggcgagcag gcctggctcg accagctcga gcacaagcag
    14101 ctgctgagcg cggtgtcgct cgacgagctc agctatctcg aagtcaaccc cgccgcgctc
    14161 gcgcttgcga gcgaggaagt gctggagcgc ttccgtcgcc agcttggcac cgtcgccgtg
    14221 cgccggcttg cggcggtgtc gcgccaggcg gccgagcacg cccccgcggc ggtgcgtgcg
    14281 gtgcgcgcgg cgccggtcgt gcacgacctg cagatcgtcg actactgaat gaattgcggg
    14341 tggagtccgc ccgcacccgc ctgaatccct ccgagccggg ctgtttcggc cagtctgtgc
    14401 gggcgggcat cgcggctact gcccggcgcc gagcgtgcgt gcgcgctcga gtgcggcgct
    14461 gcgcgcggcg gcgtccggcg cggcgatctc cagccagttg cccaggtgct tgcgcaccag
    14521 cgcggccagc gccttgatcc aggcgtcgcg ctcgttcagg caggggatgt agtggaaggc
    14581 ctggccgccc ttctccagga aggcttcctt gcactcgagc gcgatctcct ccagcgtctc
    14641 caggcagtcc gcgacgaagc ccgggcacat cacgtcgacg cggcgcacgc cctcggcggc
    14701 gagctgttcc agggtgggct gggtgtaggg cttgagccat tctgccttgc cgaagcgcga
    14761 ctggaacgtg accagtacgc gctcgcgtgc cacgccgagg ccttcggcga cgaggcggcc
    14821 ggtcttgtgg cactcgcagt gataggggtc gccgagatcg agcgagcgcc gcggcacgcc
    14881 gtggaagctc agcagcaggc gctcgggctc gccgttggcc gcccagtgct cgcgcacgct
    14941 cttcaccagc gcatcgatgt agccggggtc gtcgtggaaa ttgcgcacga agcgcatctc
    15001 gggctggttg cgccagcgcg ccaggctgct gccgacctcg tcgaccacgg tggcggtggt
    15061 gctcgcggaa tactgcgggt acatcggcac caccaggatg cgggtgcagc cgcgcgcgcg
    15121 catcgcgtcc agggtgtcgc cgatcgcggg tgcgccgtag cgcatcgcgt aatcgatgtc
    15181 gagcccgtcg tagccgtcct tggcgagata gccggcgagc agcttggtct gacgtacggt
    15241 gtgcaccagc agcggtgagc cctcgtcggt ccagatgctg gcgtatttct gtgccgaggc
    15301 cttgggccgc gtgttgagga tgatgccgtt gaggatcggc caccacacca ggcgcgggat
    15361 ctcgaccacg cgcgggtcgg aaaggaactg cttgaggtag ggacgcagcg cggcggcggt
    15421 gggggcggcg ggggtgccca ggttgaccag cagcacgccg gtgcgcggcg tgctgtcgtg
    15481 acggtggatc ggttcgggcc ggtaacgggg catcgtggtc ctcgggtggg ggcgtgcgca
    15541 cggcaaggca ggcgcacgcc ggaatcagtg cttggaggag agggcgttcg agagcagccg
    15601 ggcggtgatg tcgacgatcg ggatcacgcg gtcgtaggcc atgcgggtgg ggccgatgac
    15661 accgagcgag ccgaccacct ggccgtcgac ctcgtagggc gcggtgatca cgctgcagcc
    15721 gtcgagcggg gcgaggccgg attcgccacc gatgaagatc tgcacgccct cggcgcggtt
    15781 ggacaggtcg agcagctgga gcaggccggt gcgctgctcg aagagcctga agagctcgcg
    15841 cagctgcgac atgttgctcg acaggtcctc gacgtcgagc agcttggttt cgcccgaaag
    15901 tacgtagctc gccgcgttgt cttcggcggc ttccgagccg gcgtcgacgg tcgcggtcat
    15961 cagttcgctc atgtcgctgc gcagctggcg cagctcgtcg ccgaggcggc ggcggatctc
    16021 ctcgaaggac aggccggcga agtgctcgtt gaggtaggcg gcagcgccgt ttagctcggt
    16081 cggcgtgtag gcgcgccggg tgatcagcag gcggttctgc acgtcgccgc cggtggtgac
    16141 gatgatcagc aggatgcgat tttcggccag gctgatgaac tcgaactggc ggatgcgcac
    16201 cgcgtccttg cgcggtgcca ccaccacgcc ggcgaactgg gtgagttcgg agagcaggtg
    16261 cgaggccgag ctgatcaggc gctgcggctg gttgggcagc agctggcctt cgagggcgcg
    16321 cacgcgggcg ttctcgagcg gctgcacggt gagcagcgag tcgacaaaga aacgatagcc
    16381 gagcgcggtc gggatgcggc ctgccgaggt gtgcgggctg gcgatgaagc ccatctcctc
    16441 gaggtcgctc atcacgttgc gcaccgtcgc cggcgacagc tccagaccgg aatagcgcga
    16501 cagcgcgcgc gagccgaccg gctggccgtc cgcgatgtgg cgttcgatca gggtcttgag
    16561 gaggatctgg gagcgtgcgt cgagcggatt catggctgcc tggagtcgag aggccgattc
    16621 tattgaagca cgcccgcgct ggccactgcg ggccaccgcg cgcgggcgcg gcggtgtcaa
    16681 gcgcggggac gattgtggtt taatcggcgc ccatgagcac gccattccgc acgcttgccc
    16741 tcatcggcaa atatcagagc cccgacgtgg ccgagtcggt gctgtcgatc gcccgcttcc
    16801 tgcgcgatcg cggccttgcg gtgttgatcg agcagggcac cgcgagttcc atcggcggcg
    16861 cccacgactt cccggtggca agctacgagc atatcggcgc gagcgccgac ctcgcggtgg
    16921 tgatcggcgg cgacggcacc atgctgcaca ccgcgcgccg gctgatcgag cacggcgtcc
    16981 cgctggtggg cgtcaacctc ggccgcctcg gcttcctcac ggacatcgcg cgcagctcgg
    17041 ccaccgagcg cctcgccgag atcctcgacg gcgccttcac cgccgaggac cgtttcatgc
    17101 tcgacgtcga ggtgctgcgc ggcggcgcac gcgtgttcca cacgctcgcc ctcaacgacg
    17161 tggtggtgaa caagggcgag ctcgggcgca tgatcgagtt cgagctgtcg atcgacgagg
    17221 agttcgtcta cacccagcgc tcggacggca tgatcgtatc cacccccacc ggctcgaccg
    17281 cctacgcgct gtcggccaac ggcccgatcc tgcacccgag cgtgggcggg atcgcgctgg
    17341 tgccgctgtg cccgcacgcg ctcaccgcac gcccgatcac gctgcccgac tcctgccgca
    17401 tcgacatcgt gctgctgccg ccgcacgacg cccgggtgca cttcgatggc cagacccgtt
    17461 tcgacctgcg cgcgggcgac tgcgtgcgca tgacacgctc gagccgcagc ctgcgcctgc
    17521 tgcatcccga gggctattcc tacttcgcga tgctgcgcca gaagctgcac tggagcgcgg
    17581 cgccgagggt gtgagcgcac ccgccgcccg atctccgcgc cgccactcct caggccccgt
    17641 cgtcccgtcc caaccccacc cgcgaccgcc gctgccatgc tccgccgcct gaccatccgt
    17701 gacttcgtca tcgtcgatcg cctcgacctc gaattcgccg ccggcttcgg tgcgctgacc
    17761 ggggagaccg gcgccggcaa gtcgatcctg ctcgatgcgc tcgggctcgc gctcggcggg
    17821 cgcggcgagg cagccatggt gcgcagcggc cgcgagcgcg ccgacgtggc cgccgaattc
    17881 gacctgcccg ccggcgaggg actcgctgcc tggctcgtcg agcaggaact gcccgccgac
    17941 gagggcatgc tgatcctgcg ccgcacgatc gatatgggcg gccgcagccg cgcgtggatc
    18001 aacggcgtgg cggccacgct cgctcagctg cgcgccgccg gcgagtggct ggccgacatc
    18061 catggtcagc acgcccacca cgcgttgctg cgcaccgacg cgcagcgtgc cctgctcgac
    18121 gcccaggcgg gcgcgggggc gctggtcgcc gaggtggccg cgcgccaccg cgagtggcag
    18181 cgcctgctgc gcctgcagcg cgaagcggag gccgactcgg cgagctcggc gcgcgagcgc
    18241 gagctgctcg cctggcagct gcgcgagctc gagcaggtcg gcttcgacat cgaggaatgg
    18301 gcggagatca atgccgagca cgctcgcctg gcccacgccg cgagcctgat cgagggggcg
    18361 gaggcggcgg tggtggcgct cggcgagggt gaacttgcgg tgtgctccgt gctgggccgc
    18421 ctcgacggcc gcatcgccgc gctggccgac ttcgacgccg ggctggcgga tgtgcgcgaa
    18481 ctgatctccg ccgccgccat ccaggccgac gaggccctgc acgccctgcg ccgctatcgt
    18541 gaccggctcg acctcgatcc gggacgtctc gccgagctcg agcgccgcat cgccgcggtc
    18601 atggaggcgg cgcgcaagta tcgcgtccag cccgaggagc tgcccgcgct cgcgcagcag
    18661 tggcgcgagc gcctggacac attggaagcg accgccgatc cggcgcgcct tgccgcggcg
    18721 gcgcaggcgg cgcgggccgc cttcgagtcc gtcgcggccg agctcaccgc ggcgcgcctc
    18781 cccgccgcgc gccggctgtc ggaggagatc gccgccgcga tgcaggacct ggcgatggcg
    18841 ggtggccgct tcgaggtcgc gctcacacct tgcgcaccct ccgcggcagg cgtcgaggag
    18901 gtggagttcc gtgttgccgc gaatgccggc cagccgctgc gcagcctcgc caaggtggct
    18961 tccggcggcg aactgtcgcg catcgggctg gccatccagg tcatcaccag ccgcgacagc
    19021 gcggtgccca cgctgatctt cgacgaagtg gacgtgggca tcggcggtcg cgtcgccgag
    19081 atcgtcggca agctgctcgc ccgcctcggg caggatcgcc aggtgctttg cgtcacccac
    19141 ctgccccagg tggcggcctg cgcggactgg cagtggcgca tcgccaagcg cgagcagggc
    19201 ggcgagacgc tgagcgaggt cgcgccgctc gacgacgcgg cgcgggtgga ggaggtcgcg
    19261 cgcatgctcg gcggcgtgaa tatcaccgcg accacccgcg aacacgcggc cgagatgctc
    19321 ggccggcgct gattttcgcg tcgtccacgc gccctcggcg ctggcgcgca tccgtcgcga
    19381 tgcctcggtc cggcgcggct tcagtccttc tcgcgcccga ggttggggca gttctcgcgc
    19441 tggcagtcgg cgtagatgta gagcgcgtgc tcgcggatcg cgaagccgcg ttcgagtgcg
    19501 atctggttct gccgacgttc gatctcggcg tcgaagaact cctcgacctt gccgcactgc
    19561 atgcacacca gatggtcgtg atggccgcct tcggcgagtt cgaacaccgc cttgcccgac
    19621 tcgaagtagt ggcgctcgag cagtccggcc tgctcgaact gcgtcagtac gcggtagacc
    19681 gtggccaagc cgatgtccat gccttcgttc atcaacgtgc ggtatacatc ctccgcggtc
    19741 aggtggcgct gctccgagct ctggaagagg tcgaggatct tcaggcgggg gtaggtcgcc
    19801 ttcaggccga tgcttttcag gttcctggag ttgtcggaca tgggctctgg atgaggttga
    19861 cttgcgggag atcgggttgc gggagcctgc gaaggccgtt tggccgaccg ttgtccaggg
    19921 actcgcggga ggtcgatgcc agcccgttac ggtgtgcgcc gcagcaggca ggagcccccg
    19981 cgggcagtct ccggcaatgg cggtcgagcg ccgtcgatcg cggctatgtt atattttttg
    20041 cccctaactg agaactgcga ccccggtcgc atcccggcgc ccactctccc atgcgttccc
    20101 tcctcgctgc gctcatcgtc tcttccggcc tgcttgcagg ctgctccttc gactccgttg
    20161 tcggcctggt cgacccctac cggatcgaca tccgccaagg caattacgtc gaccaggaga
    20221 tggtcgcgca gcttcgccgc ggcatgaccc gcgaccaggt gcgctacgtg ctcggctccc
    20281 ccctggtggt cgacatgttc cgcaaggacc gctgggatta cgtctatcgc ttccgcccgg
    20341 gcagtggcga ggcgcagcag cgcgtgatct cgctgttctt cacgggcgac gtgctcgacc
    20401 gcatcgaggg cgacgtcgct gccgcctcgg gtacgcccgc cgcggcggcg caggagcgct
    20461 cgcgcgtggt cgagatcccc cccgccagcg cgcgctgagg cggcgcccgg gttcatcccg
    20521 ggtcggccgt ccgcgccggc ccattgatgc aggaaaacaa tccgatgtcc cagattcgag
    20581 tcgcgatcgc cggtgcctcc gggcgcatgg gcagaatgct gatcgaggcc gcgctgaagg
    20641 aggagggcgt cgtgctcgcc gccgccttcg atcgcagcga cacccccttc atcggtcgcg
    20701 atgccggcga gatggccggc gtggccaccg gcgtgctcat caccgacgat gccgccgccg
    20761 cgatcggcgc ggccgattgc gtgatcgact tcacccgacc cgagggcacg ctcgcgcacc
    20821 tcgcgctcgc gcgtgccgcc ggcaaggcga tggtgatcgg cacgaccggt ttcgatgccg
    20881 ccggcaagac cgcgatcgcc gccgctgccg agcacgttcc cgtggtcttc gcgcccaaca
    20941 tggcggtggg cgtgaacgcg gtgttccgct tgctcgaggt cgccgcgcgc atcctctccg
    21001 agggctacga cgtcgaggtc atcgaggccc accaccgctt caaggtcgat gccccctccg
    21061 gcacggcgct gcgcatgggc gaggtcgtcg cccgcgagct tggacgcgac ctcgagacct
    21121 gcgctgtcta tggccgcgag ggccacaccg gcgagcgcag cgccgagacc atcggctttt
    21181 cgacgatccg tggcggcgat gtggtgggtg accacacggt gctgttcgcc ggcatcggcg
    21241 agcgcatcga gatcacccac aagtcgggga gccgcatgcc ctatgcgctc ggctcgatgc
    21301 gggcggcgcg ctttctcgcc ggtcgtgcga aggggctcta cgacatgcag gacgtgctcg
    21361 gcctgcgctg aggcctgcca tgagcggcgc ggccgaatcg cgcgggagtc ctcccgatcc
    21421 cggttccgtc gaggcggagc tctcccgcct gcgcgccgag cgcgatgcgg cgctgcgcag
    21481 cctggcgcgc gcgcgccggc acctcgacct ggtgtcggcg aatgcgggtg ccggcgtgtg
    21541 gtacatggac ctgcgtggcg gctcgatccg gctcgacgaa cgctggcgca ggctgctcgg
    21601 ttacgacgga ggggatgcgg gcgactggat ggcgctggtg gacgacgagg gcagggcggc
    21661 gctgcagcag gcgctgcgcg cccacctcgg cggagaagcg gccgagctgc gcgcggagat
    21721 ccgcattcgc gcgcaggacg cgagctggca ctggatcgag gtgtgcggca acgcgagcgg
    21781 ccgcggcgag gacgggcgct gggcgcgcgt cgagggcacc taccgtgatg tcaccgagcg
    21841 caagctgcgc gagatcgaac tgctccaggc caaggaggca gccgaggcgg cgagccgggc
    21901 caagggcgac ttcctcgcca acatgagcca cgagatccgc acgccgatga acggcatcat
    21961 cggcatgacc gacctgctgc tcgactccac gatcgcgggc gagcagcgcg agtacctgca
    22021 gacggtcaag tcctcggccg aggccttgct gaccatcatc aacgacatcc tcgacttctc
    22081 gcgcatcgag gctggccgcc tgagcgtcga gcagatcgac ttctcgttgc actcggtggt
    22141 gtcggagacc tgtcgcgcgc tcgcgctgcg tgcttcgcag aaggggctgg agctctacta
    22201 cgacgtcgcg cccgacgtgc cctcggtgct gcgcggcgac cccacccggc tgcgccagat
    22261 cctggtgaat ctcatcggca acgcgatcaa gttcaccgag cacggcgagg tcgaggtgcg
    22321 cgtgcgctgc gtccagcgca acggcggcgc ggccagcatc gagttcctcg tgcgcgacac
    22381 cggctgcggc atcgcgcccg acaagctcga ggcgatcttc ggtgccttct cgcaggcgga
    22441 caattcgacc acgcgcaagt acggcggcac gggcctcggt ctggcgatct gccgccagct
    22501 caccgagctg atggggggcc ggatcggtgt cgatagcgtg ccgggtgcgg gcagccgctt
    22561 cgaggtgacc ctgccgctgc gcgcggtcgc cgacccgacc ccgctgcgcg tcggcgcact
    22621 cgccgggacg cgggtgctgg tggcgagcgc gaacgccgcc ttcgccgatt tcctgtgcgc
    22681 gaccctgcag gaggccgccc tgcgtgccga ggcggcctgc agtggcgtgg cggtggagca
    22741 ggcgctggcg cgcgccgcgg acggcagcga tcccttcgcg ttcctgctga tggatgcgga
    22801 cctgccggag ccgggcggct tcgcgctggc gcagcgcttc gccgccgcgt ggccgcggcc
    22861 ggaccgcatc attccgatga tggcgagcca ctcgcagcgc aacgatgccc tgcgctgcga
    22921 ggagctcggg ctcggctacc gcctcgccaa gcccttctcg gccgaggacc tgctggccgt
    22981 gctgcgcatg gcgcagggcg gcgagcgcga ggctgccgag ccgccgccct tcgagctcgc
    23041 accggtcgag ctgcgctcca cggtggggat ggagccgccg gcgcgcgtgc tcgaggtatt
    23101 ggtggtcgag gacaacccgg tcaaccagac cgtggccaag cgcatgctcg agcacgtcgg
    23161 ccacagcgtg acggtggcga acaacggtga ggaggccctc gaggcgatcg acaaccgtca
    23221 cttcgacgtg gtgctgatgg acgtgcagat gccggtgatg ggcggcatcg aggccacgca
    23281 ggccatccgc gcgcgcgagg cgcggcgcag ctgggtgctc gcgggcgact ggaagccggt
    23341 gccgatcatc gcgatgaccg cgcacgcgat ggagggcgac cgcgagcgct gcctgcaggc
    23401 aggcatggac gactacgtcg ccaagccggt ccgccccgag gcgctgttcg ccgcgatcga
    23461 gcgcgtgctg ggtccggcgg atgccgacga ggccgccggc gacatcaccc tgctcgagct
    23521 cgatcctgcc gaccgtcgcc gcatcgcctg cctcgacgac gcgcgcgcga tgttcgcagg
    23581 cgaggacggg gtggtcgagc aactcgtcga ggtcttcctg cgcgatctgc cgcgcacggt
    23641 cgccgacttg cacaagggcg ccgccgacat ggatttcgcg cgcctgcgcg agctcgggca
    23701 catgatcaag ggctcggtcg gggtgttctc ggcgcagcgt gcggtggatg ccgcgcgccg
    23761 tctggaggag ctggccgcgg cgggcgatcc gcgcgcgctg accagccagg cgggccttgc
    23821 ggcgcgcgag atcgagctgc tcggcgaggt tctgcgggaa gaaacggcct gcggcaggtg
    23881 agcggaggtg cgcgggcgat ttgccttgaa tccgccggaa atatacaatt gaaaggtttt
    23941 cgaagcggga tcggcccgga ggcccgtccc gctttttgta catatcgggc gcgtcgttcg
    24001 cgtgcccgca tgacttccca agcagattcc aggagcttct cgtgagtgca ttcccgcccg
    24061 cccttcttgc gctcgccgat ggaaccgtct tccacggcaa gggtatcggc gcggttggca
    24121 gcacggtcgg cgaggtggtg ttcaacacct cgatgtcggg gtatcaggaa atcctgaccg
    24181 acccgagcta ttgccgccag atcgtcaccc tgacctatcc gcatatcggc aacaccggca
    24241 tcaaccccga ggacatcgag tcctcgcgcg tgcaggcggc cggtctggtc atccgcgacc
    24301 tgcccatcct gccgtccaac tttcgcatga cccagcgcct ggacgcctac ctgcgcgccg
    24361 agaaggtggt cgcgatcgca ggcctcgaca cccgcaagct cacccgcgtg ctgcgcgaga
    24421 agggtgcgca ggcgggctgc atcgtcactg cggccgcggg ccaggcgctc gacgccgagg
    24481 ctgccgtggc ccaggcgcgc gcctttcccg gcctcgcggg catggacctc gccaaggtcg
    24541 tcagtgtcgc cgagcgctat gaatggaagc aggccgagtg ggcgctcggg agcggctaca
    24601 gcgaccgcgc cgacgggcgc ttccacgtcg tcgcctacga cttcggcgtc aagtacaaca
    24661 tcctgcgcat gctggtcgag cgcggctgcc gcctcacggt ggtgccggcg cagacgccgg
    24721 catccgaagt gctggcgctg aatccggatg gggtgttcct gtccaacggc cccggcgacc
    24781 ccgagccctg cgactacgcg atcgccgcga tccgcgagct cgtcgccgcg cgcatgccga
    24841 ccttcggcat ctgcctcggc caccagctgc tggcgctggc ctcgggcgcg aagaccatga
    24901 agatgcccac cggtcaccat ggcgccaacc atccggtcaa ggatctcgac accggcaaag
    24961 tgctgatcac cagccagaac cacggcttcg cggtcgatcc ggcgaccatg ccggcgaacc
    25021 tgaaggtgac gcacgtatcc ttgttcgatg gcaccaatca ggggattgcg cgcaccgacg
    25081 tgcccgcctt ctccttccag ggacacccgg aagcgagccc cgggccgcat gacgtcgcct
    25141 acctgttcga ccgcttcatc ggtctgatgg aagccgccaa gtaacccggc ccgcggcagg
    25201 ctgcggcctg ccccccagtt tttgacgcgc gcgacagtcg cggcgtcgag gaatcatcga
    25261 ggcaagacaa tgccgaaacg tacagacatc aagaccatcc tgatcatcgg cgccggcccg
    25321 atcatcatcg gccaggcctg cgagttcgac tattccggcg cccaggcctg caaggccctg
    25381 cgcgaggaag gctacaaggt catcctggtc aactcgaacc cggcgaccat catgaccgac
    25441 ccggagacgg ccgacgtcac ctacatcgaa ccgatcacct ggcaggtggt cgagcgcatc
    25501 atcgacaagg agcgtcccga cgcgatcctg ccgaccatgg gcggccagac cgcgctcaac
    25561 tgcgcgctcg acctcggtcg ccacggcgtg ctcgccaagt acggcgtcga gctcatcggc
    25621 gcttcggaag aggcgatcga caaggcggag gaccgcctca agttcaagga cgcgatgacc
    25681 aggatcggtc tcggctccgc gcgctcgggc atcgcccaca gcatggaaga ggcgctccag
    25741 gtccagggtg gcatcggctt cccggtgatc atccgcccga gcttcacgct cggcggcacc
    25801 ggcggcggca tcgcctacaa catggaagag ttccaggaga tctgcaagcg cggcctcgag
    25861 gcgagtccga ccaacgagct gctgatcgag gagtcgctgc tgggctggaa ggaatacgag
    25921 atggaggtcg tgcgcgatcg cgccgacaac tgcatcatcg tgtgctcgat cgagaacctc
    25981 gacccgatgg gcgtgcacac cggcgactcg atcaccgtcg cgccggcgca gacgctcacc
    26041 gacaaggaat accagatcat gcgcaacgcc tcgatcgcgg tgctgcgcga gatcggggtg
    26101 gacacgggcg gctcgaacgt gcagttcgcc atcaatcccc aggacggtcg catgatcgtc
    26161 atcgagatga acccgcgcgt gtcgcgttcg tcggcgctgg cctccaaggc caccggtttc
    26221 ccgatcgcca aggtcgcggc caagctggcg gtgggctaca ccctcgacga gctgcgcaac
    26281 gacatcaccg gtggcgccac cccggcgtcc ttcgagcctt cgatcgacta cgtcgtcacc
    26341 aagatcccgc gcttcgcgtt cgagaaattc ccccaggcca acgaccgcct gaccacccag
    26401 atgaaatcgg tgggcgaggt catggcgatg ggccgcagct tccaggaatc cttccagaag
    26461 gcgctgcgtg ggctggaggt cggcgtgtat ggcctggacg aggtcgaggc cgaccgcgag
    26521 gatctcgagc acgagatcgc cagcccgggc gcgcagcgca tctggtacgt gggccaggct
    26581 ttccgcgagg gtatgagcct cgagcaggtt ttcaacctgt ccaagatcga cccctggttc
    26641 ctcgcccaga tcgaggacat cgtgctctcc gagaaggcgc tcgccgggcg ctcgctgaag
    26701 gcgctgcagg ccgccgagct gcgcgagctc aagaagaagg gcttctccga ccgccgcctg
    26761 gccaagctgc tcaatgctga cgagaccgcg gtgcgcctgc atcggcatac gctgggcgtg
    26821 cgcccggtgt tcaagcgcgt cgacacctgc gcggccgagt tcgccacctc caccgcctac
    26881 ctgtattcgt cctacgagga cgagtgcgag gcgatgccga ccgagcgcaa gaagatcatg
    26941 gtgctcggcg gtggtcccaa ccgcatcggc cagggcatcg agttcgacta ctgctgcgtg
    27001 catgcggcgc tcgccctgcg cgaggatggc tacgagacca tcatggtcaa ctgcaacccg
    27061 gagacggtgt ccaccgacta cgacacctcg gaccgcctgt acttcgagcc gatcacgctc
    27121 gaagacctgc tcgagatcgt gcacatcgag aagccggtcg gcgtgatcgt gcagttcggc
    27181 ggccagactc cgctcaagcg cgcgcaggcg ctcgaagcca acggcgtgcc gatcatcggc
    27241 accagccccg acatgatcga cgccgccgag gaccgcgagc gtttccagca actgctcgtc
    27301 gagctcggtc tgcgtcagcc gcccaaccgc accgcgcgga cgcccgccga agccgtgcgc
    27361 ctcgcggccg agatcggcta tccgctggtg gtacgcccgt cctacgtgct gggtggccgc
    27421 gcgatggaga tcgtgcacga gcagaaggac ctcgagcgct acatgcgcga ggcggtcaag
    27481 gtctcgaacg agtcgccggt gctgctcgac cgcttcctca acgacgccac ggaggtggac
    27541 gtcgatgcgc tgtccgacgg caagcaggtg atgatcggcg gcatcatgga gcacatcgag
    27601 caggccggcg tgcattcggg cgactcggcc tgctcgctgc caccctatac cctgtcggcg
    27661 aggctgcagg acgagttgcg ccgccagacc gaggccatgg cgcgcgcgct gaaggtcgtc
    27721 ggcctgatga acgtgcagtt cgcgatccag ggcgagggcg acaatgcggt cgtctatgtg
    27781 ctcgaggtga acccgcgcgc ctcgcgcacc gtgcccttcg tctccaaggc ctgctcgctg
    27841 ccgctggcca agatcgccgc gcgctgcatg gcgggtcaga gcctggccga gcagggcgtc
    27901 agcggtgaga tcgtgccgcc ttactactcg gtcaaggagg cggtgttccc gttcgtgaag
    27961 ttccccggcg tggataccat cctcgggccc gagatgaagt ccaccggcga ggtcatgggc
    28021 gtgggccgca gcttcgccga ggccttcgtg aagagccaga tgggcgcggg cgtgcgcctg
    28081 ccaaccgcgg gcacggtgtt catcagcgtg cgtccgaccg acaagccggt cgcgatcgag
    28141 gtcgcgcgcg agctgcacga gatgggcttc gcgctggtgg ccacccgcgg caccgcagcg
    28201 gcgatcgagg ccgccgggat cccggttggc gtggtcaaca aggtcaacga gggccgtccg
    28261 cacatcgtcg acatcatcaa gaacgaggag atcgtgctgg tgatcaacac cgtcgatgag
    28321 aagcgccagg cgatcgctga ctcgcgctcg atccgcacca gcgcgctcgc cgcaaaggtg
    28381 tcgatcttca ccaccatcga gggcgcgcgc gccgcgtgca tgggcatgcg ccacctttcg
    28441 aacggtctcg aggtgtattc cgtgcagggc ctgcacgccg aactgaagca gcaagcatga
    28501 acaagacccc gcttaccgtc gccggcgccg agaagctgcg caccgaactc catcgactga
    28561 agaccgtcga gcgccccaac gtgatcgccg cgatcgccga ggcgcggtcg cacggcgacc
    28621 tctccgagaa cgccgagtac gaggctgcca aggatcgcca gggcttcatc gagggccgca
    28681 tcatggaggt cgagtccaag ctcgccaacg cgcagatcat cgatccgaca ctgctcgatg
    28741 cggacggtcg ctgcgtgttc ggcgccacgg tcgagctcga agacctcgag tccggccagg
    28801 tcgtgaccta ccagatcgtc ggcgacgatg aagccgacat caaggacaac aagatctcga
    28861 tcagctcgcc gatcgcgcgt gcgctgatcg gaaaatacgc gggggacatc gccgaggtgc
    28921 aggcgcccgg cggggtgcgc gagtacgaga tcctggacgt gcgctacgtc tgatctgcac
    28981 cttgccggcg tggcgatgaa gagggcggtg cgatgcaccg cccctttgtt tgcagcctcg
    29041 gcgcaaggcg agccgggcgg gtgggcgagc cgggcgggtg cggctcagcg gccgcgtgcg
    29101 gcgccgcgtc cgcgcggggc gggcgcacgt gccggcgagc ggcgcgactc ggcgctcgcc
    29161 ttggcgagcg cggcacgacg cgcggcggcg gcgaaggcgg cggccgactt ggcggtggaa
    29221 gcgcgcacag gcttcttctc tgcgggcgcg gagctcttgt cgtcctcgcg gcgcgcacgc
    29281 cagacgacga ggatgctgcc gatgtgctgc accggggcgg cgtccaccgc ggcgcacagc
    29341 tcctgcatca aggcctcgcg ctgctcgcgt tcggcgccct ggaccttgac cttgatgagc
    29401 tcgtgcgcct gcagggcgcg ttcgatctcg gcgaggacgg ccggcgtgag gccgttgccg
    29461 gctacggtca cgacggggtt caggtggtgg gcacgggcgc gcagctcgct gcgctcggcc
    29521 ggggaaagct cggtcattgc aaatctctgg atgggtgaag cccgggacgc ggattctacc
    29581 ccgtacgtgc caaggatggc cgtcgcggat ggagcgagga tgaagaagaa caagaccagc
    29641 cgtgcctggg tgcacgatca tgtgaacgat ccctgggtgc aacgcgccca ggccgagggt
    29701 tatcgcgcac gcgccgcctt caagctgctc gagatcgacg agcgcgacca cctgctgcgt
    29761 ccgggcgcgg tggtcgtcga cctgggagcg gcgcccggct cgtggtgcca gcttgcggtg
    29821 aagcgctgcg ggcccaaggg acgcatcttc gcgctcgacc tcctgcccat ggagccggtg
    29881 gcgggggtgg agttcctgct cggcgacttt accgaggatg cggtgctcga ggagttcgag
    29941 gcgcgccttg gtggcgcgag cgtggatgtg gtgctttcgg atatggcgcc gaacctttca
    30001 ggggttgcga cggtcgacca ggcacgttcg gtacatcttt gcgaattggc gctggacttc
    30061 gcggtgcgtc atctcaagcc gggtgggcag ttcgtggtca aggtgttcca gggcgagggc
    30121 ttcatggagt atcgcaagca gatggaggcg gcgttcgtct cggtgcaggt gcgcaagccc
    30181 aaggcttcgc gcgaccgtag cgccgaggtc tatctcgtcg gccgggggcc gcgccggcct
    30241 tgagttcgca tgcgggggtg gcgattgggt tgcgctatgt gccccattgg tttgcccgtg
    30301 ggaacaatcg ccactagaat cggtcagtcg cgtacaggcc ggaatggctt tcggggaaca
    30361 gaaagttgaa caatctcttc aagaatctcg cgatctggat ggttatcggc gtcgtgctga
    30421 tgacggtatt caaccagttc aacacgcgcc agacgccggc cagcaccatg gagtattccc
    30481 agttcctgga ggaggccaag gcgggacgga tcgcgcgcgc gaccgtcgat ggccgcgtgc
    30541 tcaaggccac cacccaggaa gggcgcacga tcacggtgta tacgccgggc gtgcaggaca
    30601 tctggatgat ttcagacctg atgcgctacg gcgttcagat caacgccagc aagcccgagg
    30661 aggagcagtc cttcctcgcc agcgtattcg tgtcgtggtt cccgatgttg ctgctgatcg
    30721 gcgtgtggat cttcttcatg cgccagatgc agggcggggg caagggcggg gcgttctcct
    30781 tcggcaagag caaggcgcgc atgctcgacg agtcggccaa ttcggtgacc tttgccgacg
    30841 ttgccggctg cgacgaggcc aaggaagaag ttgccgagct ggtcgagttc ctgcgtgatc
    30901 cgtccaagtt tcagaagctc ggcggccgca ttcccaaggg cgtgctgatg gtcggttcgc
    30961 cgggtaccgg caagaccctg ctcgccaagg cgatcgccgg agaggccaag gtgcccttct
    31021 tctcgatctc cggctccgac ttcgtcgaga tgttcgtcgg cgtaggcgcg gcgcgcgtgc
    31081 gcgacatgtt cgagcaggcc aagaagcagg cgccctgcat catcttcatc gacgagatcg
    31141 acgcggtcgg ccgccagcgt ggtgcgggtc tgggcggcgg caatgacgag cgcgagcaga
    31201 cgctcaacca gttgctggtc gaaatggacg gtttcgaggg ccagaccggc gtgatcgtga
    31261 tcgccgcgac caaccgcccc gacgtgctcg acccggcgct gctgcgcccg ggccgcttcg
    31321 accgccaggt ggtggtggcg ctgcccgaca tccgtggtcg cgagcagatt ctcaaggtgc
    31381 acatgcgcaa ggtgccgatc gcgcccgacg tcgatccgca ggtgctggcg cgcggcacgc
    31441 cgggctttgc cggtgccgac ctcgccaacc tggtgaacga ggctgcgctg ttcgccgcgc
    31501 gcgccaacaa gcgtctggtc gacatggagg acttcgagcg cgccaaggac aagatcatga
    31561 tgggcgccga gcgccgctcg gtggtcatgc ccgaggagga gcgccgcaac accgcctacc
    31621 acgagtccgg tcacgcggtg gtggcgcgtc tgctcgacaa gaccgatccg gtgcataagg
    31681 tgaccatcat cccgcgcggc cgtgcgctgg gcgtgaccat gcagttgccg accgaggatc
    31741 gctacagcca ggaccgcgat cgactgctgc agaccatcgc cgtgctgttc ggtggccgca
    31801 tcgccgaaga gatcttcatg aagcagatga cgaccggtgc gtcgaacgac ttcgcccgtg
    31861 ccactgacct cgcgcgccgg atggtgacgc agtggggcat gtccgacacg ctcggaccga
    31921 tggtgtatgg cgaggaggag ggcgagatct tcctcggccg ccaagtcacc acgcaccgca
    31981 acgtgtccga ggcgacgatg cagaaggtcg acgcggagat ccgccgcatc atcgatcagc
    32041 agtactcgct ggcgcggcgc ttgctcgaag agaacagcga caaggtcgag gcgatgacgg
    32101 ccgcgttgct ggaatgggag accatcgacg ccgagcaggt gaatgacatc atggccggcc
    32161 gcccgccgcg tccgccgaag ccggcatcgg cgccgtccag ccgtccgaag gacgacgcgc
    32221 cgggtgcggc gcccaccgcg ccagcacctg ccgcctgagt tcgacaactt ccgtttcaag
    32281 gggccggccg gaccggcccc tttccattcc ggcgtccgcg cgcctgcgcc cggacgcctt
    32341 gcttttcccg aggtctcgat gtccgtcctg caatgcggcc gcttccggct cgatctgtcc
    32401 cagcccaggg tgatggcgat catcaacctg accgacgact ccttctccgg cgacggtttg
    32461 cgtggcgacc tcgcccgtgc gctcgcgcgc gccgagcgtg cgctggagga aggggcggag
    32521 atgctcgata tcggcgccga gtcgacccgg ccgggatcgg acccggtgcc cgaggcgcag
    32581 gagctcgagc gcgtggtgcg cttcgtcgag gccgcgcgcg gctggaaagt gccgctctcg
    32641 atcgacaccc tgaagccgac ggtgatgcgc gccgcgctcg cggcgggtgc ggacatgatc
    32701 aacgacatca acgcgttccg cgcatcgggt gcgatcgagg cggttgccgc gggcgagtcc
    32761 ggcttgtgcg tgatgcacat gctcggccag ccgcgtacga tgcagcagga tccgcgctac
    32821 gccgacgtgg tcggcgaggt gaccggcttc ctcgacgagc gcgttgccgc cctcgtggcc
    32881 gcaggcgtgg cgcgcgagcg catcgtgctc gaccctggct tcggcttcgg caagagcgtc
    32941 ggccacaact atgcgctgct gcgcgagctc gcgcgtgtcg gcgtcggcgg gttgccggtg
    33001 ctggcggggc tgtcgcgcaa gtccatgctc ggcgcagtca ccggtcgcgc ggtcggcgag
    33061 cggcttgcgg ccagcgtcgc cggggcgctg atcgcggtgc agaacggcgc gcgtatcgtg
    33121 cgggtgcatg acgtcgccgc cactcgcgac gcgctcgcgg tgtggaaggc gaccgtgacg
    33181 ggatgctgag caaggcaccg cccgcggtgt cataatccgc gctttcgaaa gctgctggag
    33241 cgaagatggc gcgcaagtat ttcggcaccg atggtgtgcg tggcaaggtc ggcgatacgc
    33301 cgatcacgcc cgagttcgtg atgaggctgg gctatgcggc cggcgtgacc ttggtcgcgc
    33361 gcgagaagct gcccgcgggc gaacaccccg cggtgctgat cggcaaggac acgcgcattt
    33421 ccggctacat gctcgaagcc gcgctcgagg ccgggttcgc cgcggctggt gtggacgtga
    33481 tgctggccgg accgatcccc accccggcgg tagcctatct cacgcgcgca ctgcgcctgc
    33541 aggccggcgt ggtgatctcg gcctcgcaca accccttcta cgacaacggg atcaagttct
    33601 tctccgccga cggcagcaag ctgcccgatg cggtcgaggc cgagatcgag gcccacctcg
    33661 acgagccgat gggttgtgtg gactccgccc gcctgggcaa ggcgcgccgc atcgacgacg
    33721 ccgccgggcg ctacatcgag ttctgcaaga gcacctttcc caacgagctc gacctgcgcg
    33781 gcctgcgcat cgcggtggat tgcgcgcacg gcgcggccta ccagatcgcc cccaaggtct
    33841 tccacgagct cggcgcggag atcatcccgg tgggcgtcga gcccaacggc ctcaacatca
    33901 acgacggcgt cggtgcgacg cggccggaga acctgcggca ggcggtgctc aagcatggcg
    33961 cggacctggg catcgcgctc gacggcgacg ccgaccgcct gatcatggtg gaccatcggg
    34021 gcgagatcta cgatggcgac aagctgctct acgtggtggc ctgtgcgcgc cacgccgaag
    34081 gccggctgga gggcgtggtc ggcacgctga tgagcaacct cggcttcgag cacgcgatcc
    34141 agcgcctcgg cacgcccttt gcacgcgcca aggtgggcga ccgctacgtg ctcgagctgc
    34201 tgcaggagcg cggctggaag attggcggcg agaactccgg tcacatcatc tgcctcgacc
    34261 gccacacgac cggcgacggc atcgtgtcgg cgctgcaagt gctcgccgcg ctcaagcagc
    34321 gcggactttc gctggcggac gcctgcgcgg atctggtgtt ctacccccag cgcctgatca
    34381 acgtgcgcat gcctgccggc ttcgactggc gtgcggacgg ctcgatcgcg agcgcacagg
    34441 ccagagccga acagacgctc ggcgatagcg ggcgcgtgct gctgcgccct tcgggcaccg
    34501 agcccctgct gcgcgtgatg gtggagggcc gcgacggcga gctggtcgag cgtctcgcgc
    34561 gcgagatcgc cggcgtcgtc gaggaagcga tcgcctgatt cgggctgcat gagatgggag
    34621 gacggggcgc aaggctccgt ccggacgcgg gaagaaggca gatctgcccg ttcgatgaca
    34681 cgcagcgtgg ctgcgacaat cgctggctac ctgacgggct cggtcttcaa ccccgagggg
    34741 catcccggcg gaggagccga cttcggcgct cgatccgatc tcgacggcgc gtatcgaaga
    34801 actgatcgac gaactggagg aagagtccat catcgtcgtc gtcacccaca acatgcagca
    34861 ggcggcccgg agctcggact acaccgcctg catgtacctg ggcgagatgg tggagttcga
    34921 cgtcacagac gagctcttcg tgaagccgaa caagaaggag accgaggacg acatcaccgg
    34981 tcgctgcggc cgagcaacga tcccgggcgg aagagtgcgc gcccctcgct gcacgagggc
    35041 tggtcgcatt gcgggtgatc ctccgcaagc cgtataatcc tgcggtttat ctgcacggat
    35101 attccctctc ccatgaccag actcgttgcc ggtaactgga agatgaacgg cagcctcgcc
    35161 gccaatcgcg agctgctcga cggcctcgtg tcgatcccgg gcgtggaaat cgccgtgtgt
    35221 gtgccgtatc cctatctcgc ccaggcgcaa caacggcccg agggcgtcgc cctcggtgcg
    35281 caggacgtgt cggagtacgc ggccggcgcc tataccggcg aggtgagcgc ggccatgctc
    35341 gcggagttcg gctgccgcta cgccatcgtc ggtcactccg agcgccgtgc cctcttcggc
    35401 gaggaagatg ccacggtcgg gcgcaaggcg ctcgccgcgc tgcaggctgg cttggtgccg
    35461 atcgtgtgcg tgggcgagac gctcgccgag cgcgacgagg cgcgtgcggt cgaggtgatc
    35521 ggccgccagc tcgaggcggt gcgcgaggtg ctcggcgccg cggcgatggg tgcggtggtg
    35581 ctagcctacg aacccgtgtg ggcgatcggc accgggcgct ccgcgagcgt ggcgcaggtg
    35641 gccgaggtgc atgctgcgat ccgcgcgtgg ctgaaggtac acggcgtggc ggcgggtggc
    35701 gtccgcatcc tctacggcgg cagcgtcaag gcggacaatg ccgcggcgct cttcgccgtg
    35761 gaggatgttg acggtgggct gatcggcggc gcctcgctcg tcgctgcgga tttcttggcc
    35821 atctgccgcg ccgcggcggg tggggtctga gtttcaacga ttcgtatctt tcaggattca
    35881 agatgggtga atatcttttc tctctcgtgc ttaccgtgca tgtgctggtg ggcctcggcg
    35941 tgatcgggct cgtgctcgtg cagcacggca agggtgcaga catgggtgca gccttcggca
    36001 gcggcgcatc gggcagtctc ttcggtgcat cgggttcggc gaacttcctc agtcgcacca
    36061 ctgcggtact cgccacggtt ttcttcctga ccagcctcgg gctcagcttc cttgccagca
    36121 acaagccggc ggcaccgggc agcgtgatgg aaggggcgca gactgcgccc gcaacgaacc
    36181 cggctccggc cgcgcccgtc gagtcctcgc cggcccagtc gatcccgaaa taattggttg
    36241 cgagcccttt gcgcagtgcg ggaaaacact tataattatc gcgctgtcag aacgaagcgc
    36301 cgacgtggtg aaattggtag acacgctatc ttgagggggt agtggcgaaa gccgtatgag
    36361 ttcgagtctc atcgtcggca ccacattccg ggaacgcccg cagaagcggg cgtttccacg
    36421 cagagctgag caataacaat aattgcggcg cgccaatgac gccgcggact tcaaatgggg
    36481 gtttccggac atgctggaaa attattttcc cgtcctgatg ttcatgctcg tcggcctcgg
    36541 cttcggcctt gcgccggtca tcctcgggcg actcctcgcc ccctatcgtc ccgattccga
    36601 aaagaattct ccctacgagt gcggcttcga ggcgtttgaa gacgcccgca tgaagttcga
    36661 tgtccgctac tacctgatcg ccattctctt catcctgttt gacctcgaaa tcgccttcct
    36721 ctttccgtgg gcgacggtct tccaggagtt catcgccgcc ggcgaaatcg cttggttcgt
    36781 attcggctca gtgatggttt ttctggcgat tctggtcatt ggctatatcg tcgagtggaa
    36841 aaacggcgca ctcgactggg agtaatgcat gagcattgag ggcgtcttcc gcgaggggtt
    36901 cgtcaccacc tcgctcgatg cggtcatcaa ctggacgcgc accggctcgt tgtggccgat
    36961 gaccttcggt ctggcctgtt gcgcggtcga gatgatccac gcgggctgtt cccgctacga
    37021 cttggatcgc ttcggtgttg tgttccggcc gagtccgcgc cagtccgacc tgatgatcgt
    37081 cgccggaacc ttgtgcaaca agatggcgcc ggcactgcgc aaggtctatg accagatgtc
    37141 ggagccgcgc tgggtcatct ccatgggctc ctgtgccaac ggcggtggct actaccacta
    37201 ttcgtactcg gtcgtgcgcg gttgtgaccg catcgtgccg gtcgacgtct atgtgccggg
    37261 ctgtccgccc accgccgagg cgctgatcta cggcattctc cagctccaga acaagatcaa
    37321 gcgcacgaac acgattgcgc gctgacgggt gtcccagaca tgagtgccaa gctagaacgc
    37381 ctcagccaga ccttgcgcga cgttttcggt aaccggatcc agtcgctcgt actcgatcgc
    37441 ggtgaagtga cgatcgaggt ctccgctgcc gaatatcccc gcgtcgcacg ccagcttcgc
    37501 gaccatgccg atttgcgctt cgagcagttg attgaccttt cgggtctcga ctactccgcc
    37561 tacggtaacg cgatctggga gggcaagcgc ttcgcttccg tcgcccacct gacctcggtc
    37621 aagcacaact ggcgcctgcg tgttcgcgtg ttcgccgacg acgacgcctt cccggtgatc
    37681 gattcggtgg tcgatgtgtg gccgagcgct aactggtacg agcgcgagtc cttcgatctg
    37741 tacgggatcc tgtattcggg ccacccggat ctgcgccgca tcctgaccga ctacggtttc
    37801 gtcggtcatc cgttccgcaa ggacttcccg gtttcggggt acgtcgaaat gcgctacgac
    37861 ccggagcagg gcagggtcgt ctatcagccg gtgagcatcg agccgcgcga gaacacgccg
    37921 cgcatcgtgc gcgaagagaa atacggggac gtcggccatg gctgagatcc gcaactacac
    37981 gatcaacttc ggtccgcagc acccctcggc gcacggcgtg ctccgcctcg tgctcgaact
    38041 cgacggcgaa gtcgtcgagc gcgccgaccc gcacatcggc ctcctgcacc gcggcaccga
    38101 gaagctcgcc gagacgcgca cctgggtgca gtccgtgccg tacatggacc gtctcgacta
    38161 cgtgtccatg atgtgcaacg agcacgccta ctgcatggcg atcgagaagc tgctcggggt
    38221 cgaggttccg ctgcgggccc agtacattcg ggtgatgttc gacgagatta cccgcgtcct
    38281 gaaccacctg ctcaacatcg gtacccatgc cctcgacatc ggcgcgatga ccatggtgct
    38341 gtatacgttc cgcgagcgcg aagacctgat ggacgtctat gaggcagtct cgggtgcgcg
    38401 catgcatgcg gcgtactacc gtccgggcgg cgtctatcgc gatctgccgg accgcatgcc
    38461 caaatacgag gtcaacaagt tcaagaacga gaagacggtt cgcgagctca acgccaaccg
    38521 cgaaggctcg atgctcgact tcctcgagga cttcaccaac cgcttcccga cctactgcga
    38581 cgaatacgag acactgctga ccgacaaccg catctggaag cagcgcaccg tcggcatcgg
    38641 cgtggtatcg cctgagcagg cgctggcctg gggtttcacc ggtccgatgc tgcgcggctc
    38701 gggcatcgcc tgggatctgc gcaagaagca gccctacgag gtctatgatc gcgtcgattt
    38761 cgatgtgccg atcggcaaga acggcgactg ctacgaccgt tacctgtgcc gcatggaaga
    38821 gatgcgccag tcgaaccgca tcatcaagca gtgcatcgac tggctgcgca agaaccccgg
    38881 tccggtcatc accgacaacc acaaggtcgc gccgccttct cgcgaggcga tgaagaccaa
    38941 catggaagag ctgatccacc atttcaagct ctttaccgaa ggcatgcacg tgccaaaggg
    39001 cgaggtgtac gcggcggtcg agcatccgaa gggcgagttc ggcgtgtatg cggtgtcgga
    39061 cggggcgaac aaaccctacc ggctcaagct gcgcgcgccg ggcttcgcgc acctcgcggc
    39121 catggacgac atcgcccgtg gccacatgat tgccgacgtg gtcgcgatca tcggcacgat
    39181 ggacgtggtg ttcggcgaga tcgatcgctg agcgcgcccg cgcgcaacag cggatgggcg
    39241 cctgcgggcg ccgcggtatc aaacaagaat cggcaggcta tccggaagat caagacgaca
    39301 tgctgagcca ggaatcgctg caacagatcg atcgagagat cgccaagtat ccgcccgatc
    39361 agacgcagtc tgcggtcatg gccgcgctgc ggatcgcgca ggtcgagaag ggctggctgc
    39421 ccaaggaact catcgagttc gtggccaggt acctcggcat gccgccgatc gccgccttcg
    39481 aggtcgcgag cttctacaac atgtacgacc tcgcgccggt cggacggcac aagatcaccg
    39541 tgtgcacgaa cctgccgtgc gcgctgtccg gcggcgtcca tgcggcggac tacatcaagg
    39601 aaaagctcgg catcgacttc aacgagacca cgcccgacgg caagttcacg ctgaaggaag
    39661 gcgagtgcat gggcgcctgt ggcgacgccc cggtgctgct ggtgaataac catcacatgt
    39721 gcagctggat gaccaccgag aagatcgacc agatgctggc cgagctggac aagaaatgag
    39781 cacgcaagga atgatcctcg ccggctgcga cggcgaccgc acctggcggc tccaggacta
    39841 cgtcgcccgc ggcggttatt cggcgctccg caagatcctc gccgagaaga taccgcagga
    39901 cgcgatcatc gccgagctca agacctcggt gctgcgcggt cgtggcggag cgggcttccc
    39961 gaccggactg aagtggagtt tcatgccgcg ctccttcccg ggcgacaagt acctcgcgtg
    40021 caactccgac gagggcgagc cggggacctt caaggaccgc gacatcctgc gctacaaccc
    40081 gcacagcgtc atcgagggca tggccatcgc cgcctacgcg atgggtgccg cgcggggtta
    40141 caactacatc cacggcgaga tcttcgagac ctactcgcgc ttcgaggagg cgcttgcgga
    40201 ggcccgcgag gccggtttca tcggccgcaa catcctcggc agcgacttct ccttcgagct
    40261 cttcgcgcat cacggctacg gcgcctacat ctgtggcgag gagaccgcgc tgctcgagtc
    40321 gatcgagggc aagaagggcc agccgcgctt caagccgcct ttcccggcga gctacggcct
    40381 ctatggcaag ccgaccacga tcaacaacac cgagaccttc gcgtccgtcc cgttcatcat
    40441 gaacatgggc ggcgaggcct tcctgaacct cggcaagccc aacaacggcg gcatgaagct
    40501 cttctcggtc tcgggccacg tcaatcgccc gggcaactac gagatcccgc tcggcacgcc
    40561 cttcgccgag ctgctcgaga tggctggcgg catgcgcggc gggcgcaaga tcaaggcggt
    40621 gatccctggt ggttcgtccg ctccggtcgt tcccggcggc gtgatgatgg actgcacgat
    40681 ggactacgac tcgatctcca aggcgggatc gatgctcggt tccggcgcgg tcatcgtgat
    40741 ggacgagacc acctgcatgg tcaaggccct cgagcgactg tcctacttct acttcgagga
    40801 gtcctgcggc cagtgcacgc cctgccgcga gggcaccggc tggctgtacc gtgtggtgca
    40861 tcgcatcgag cacggtcttg gccgcccgga cgacctcgac ctgctgaact cggtgacgac
    40921 caacatcatg ggccgcacga tctgcgcgct cggtgacgcc gcctcgatgc cggtgcagag
    40981 cttcgtcaag cactttggca gcgagttcga ataccacatc gaaaacaaga agtgcctcgt
    41041 gccgcccgaa gtgcagcgcg ctggcagtca aatctacgtg agcccgtcat gctagagatc
    41101 gaaatcgacg gcaagcaggt ccaggtcaac gacggcagca cggtgatcga ggctgcagcg
    41161 gttgccgggt cctacattcc gcacttctgt taccacaaga agctgtccat cgcggcgagc
    41221 tgtcggatgt gcctggtgca ggtcgagaag gcgcccaagc cgctgcccgc ctgcgcgaca
    41281 cccgttacca atggcatgaa ggtatggacg cactccgagc tcgccgtgaa ggcgcagaag
    41341 ggggtgatgg agttcctgct gatcaaccac ccgctcgact gtccgatctg tgaccagggc
    41401 ggcgagtgcc agctgcagga cctcgcagtg ggctacggtt cgaccgcctc gcgctaccag
    41461 gaagagaagc gcgtggtgtt caacaagaac cttggcgcgc tggtgtcgac cgacatgacg
    41521 cgctgcatca actgcacgcg ctgtgttcgc ttcaccaccg agatcgccgg cgagatggag
    41581 ctcggccagg ccttccgcgg cgagcatgcg gagatcatgc ccttcatcga gaagacggtc
    41641 gattccgagc tctccggcaa catcatcgac ctctgcccgg tcggtgcgct gacctcgaag
    41701 cctttccgct tcgccgctcg tacctgggag ctctcgcgcc gcaagtcggt gagcccgcac
    41761 gactccctcg gcgccaacct gatcgtccag accaagcacg acgtggtgaa gcgcgtgctg
    41821 ccgttcgaga acgaagacgt caacgagtgc tggctgtccg acaaggaccg cttctcctac
    41881 gaggccctgt ccagcgagca gcgcctcggc gcgccgatga tccgccaggg cgggcagtgg
    41941 aagcaggtcg attggcaggc cgccctcgag ttcgtcgcca ccggcctgcg cggcgtcgag
    42001 cgcgatcatg gtgccgcctc gatcggcgcg ctggcctcgc cgcacgccac ggtcgaagag
    42061 ctctacctgc tgcagaagct gatgcgcggg ctcggtagcg agaacatcga tttccgcctg
    42121 cgcgaggtcg acttccgtgc cgacggcaag cgcgccggcg ctccgtggct ggggatgccg
    42181 atcgccggcg tcaaggatct gacccgcctg ctcgtggtcg gcagcttcct gcgcaaggat
    42241 gcgccgctgc tggcgcagcg cgtgcgccag gccgcaaaga aaggcctgca cgtcagcgcc
    42301 atcggcccga acgccgaaga gtggttgctc ccggtgcgca accgcgcgct ggtcgcgcct
    42361 tcggcgatgg tggccacgct cggccaggtc gtggtcgcgc tggcgaagga gaagggcgcc
    42421 gaggtcagcg tcgcgctgct cggcaaactg cccgagacag tgagcgatga agcgctcgcc
    42481 atcgcgcaga gtctcgccag cggccgcaag gttgcggttt ggctcggcaa tctcgccgtc
    42541 cagcacgccc gcgcttccga gctgcagatg ctcgcgcagg agatcgcgcg cctcaccgac
    42601 ggtagcttcg gcttcatcgg cgaggctgcc aacagcgtcg gcggctatct tgccggcgcg
    42661 gttcccggcg cgggtggcct gaacgccgcc gcgatgatcg agcagccacg tgcggcctac
    42721 gtgaccctga accttgagcc cgatctcgac cttgccaatc cggcggcggc gatggctgcg
    42781 ctcgacggtg cccagctcgt ggtggcgctc acgagcttcg actcgccgtc gctgcggcag
    42841 gtggccgacg tgatgttgcc gatcgccccc ttcaccgaga cctcgggcac cttcgtgaac
    42901 tgcgagggcc gcgcgcagag cttcaccgcg gtggccaagc ccttcgccga tgtccgtccg
    42961 ggctggaagg tgctgcgcgt gctcggtaac ctgctcgcgc tcgagggttt cgcccacgcc
    43021 gactccgagg ccgtgcgtgc cgaggcgctc gcgggtgatc tcgccagccg gttggacaat
    43081 ggcgtggaag gtatcgtggt ctcttcggct accggtccgg caggcgtgct cgagcgtgta
    43141 gccgacgtgc cgatccactt cgccgatccg atcgcgcgcc gcgccccgtc gctgcagaag
    43201 acgcgcgacg cagcggcacc cacggcgcgc atcgcgccgg caacgctcgc ggcgctcggc
    43261 ctcgcggccg gggaccgtgt gcgcgtggca cagggtacgg cgagcgtgga actcgtggcc
    43321 caggcggatg atggattggc tgcaggatgc gtgcgcgtcg ccgccgcgca tccctcgacc
    43381 gcggcgctcg gcgcgatgag cggtgatctc agcgtggagc gtgcgtgatg gaagcgatgc
    43441 tgcaacccgt ggcggatttc ttcggcccct cctggacggc catctggacg ctgctcaaga
    43501 tcgtcgcgat catcgcgccg ctgatgattt gcgtcgccta cctgacgctc gccgagcgca
    43561 aggtcatcgg ctacatgcag gtccgcatcg gccctaaccg ggtcggtccg aagggccttc
    43621 tgcagccgat cgccgacggc atgaagttgc tgttcaagga gatcatcgtt ccgagcgggg
    43681 ccagcaaggg cctcttcatc ctcggtccca tcctggcgat cgcgccctcg ctggcagcct
    43741 gggcagtggt gcccttcgac gacggcctgg tgctcgcgga cgtcaatgcc ggtctgctct
    43801 tcctgctcgc catcacttcg atggaggtgt acggggtgat cgtcgcaggt tgggcgtcga
    43861 actcgaagta tcccttcctc ggttcgatgc gtgccgcggc gcagatggtg tcctacgagg
    43921 tgtcgatggg tttcgcgctg atctgcgtgc tgctcatctc ggccagcctc aacctcaccg
    43981 acattgtcat gtcgcagaac gaaggccgct tcgctgacat gggcctgggc gtcctgtcgt
    44041 ggaactggat tccgcttttt cccatgttcg tggtgtacgt gatctcgggc atcgccgaga
    44101 ccaaccgcgc gcccttcgac gtggtcgaag gcgaggccga ggtggtcgcg gggcatatgg
    44161 tcgagtactc gggcatgacc ttcgcgctgt tcttcctcgc cgaatacgcg aacatgattc
    44221 tggtgtcgat cctgacctcg atcctgttcc tcggtggctg gctgtcgccc gtgggcttcc
    44281 tgcctgacgg tttccactgg ctggcggtca agaccgcgtt catcctgctg ctgttcctgt
    44341 gggcgcgagc caccttcccg cgcttccgct acgaccacat catgcgtctg ggctggaagg
    44401 tgttcatccc ggtcaccctc gtgtggctgt tcgtggtgac cgtgtggatg atgtcgccgc
    44461 tgtcgatctg gagttgagaa gaccatgggt gccaaggatt acatcggcag tctgttcctg
    44521 aaggaacttg tcaagggcat ggccctgacc ggacggcatt tctttcagcg caagatcacc
    44581 atcctcttcc cggaagagaa gacgccgcag agcaaccgtt tccgtggcct gcacgcgctg
    44641 cgccgctacc cgaacggcga ggagcgctgc atcgcgtgca agctgtgcga ggcgatctgc
    44701 ccggcaatgg cgatcacgat cgagtccgat cagcgtgacg acggatcgcg ccgcacgacg
    44761 cgctacgaca tcgacctgac caaatgcatc ttctgcggct tctgcgagga agcctgtccg
    44821 gtggacgcga tcgtcgagac tcgcgtgctg gagtaccacg gagagcagcg cggcgatctg
    44881 tactacacga agcagatgct gctcgcggtg ggggatcgac acgagtcgca gattgcggca
    44941 gaccgccaac aggacgccaa gtaccgctaa ggggagggcg cctcggcgcc acgagtcaag
    45001 aacatggaat tcaagacctt cgtcttctac tttctcgccg cgatcatggt gctcgcggcg
    45061 ctgagggtga tcaccgcgcg caacccggta cacgccgcgc tcttcctcgt gctcaccttc
    45121 ttcaacgcgg gcggtctctg gctgttgctg caggccgagt tcctcgcggt cacgctcgtg
    45181 atggtgtatg tcggcgcggt gatggtgctc ttcctgttcg tggtcatgat gctcgacatc
    45241 aacctcgacc gtctgcgtga aggcttctgg agctatctcc cggtgggagc cctggtgggc
    45301 atcctgatgc tggtcgagat ggtgatggtg ctcggcggcg gctacttcgg tctcgaggcg
    45361 atgcctgcgc ccgctccggt tgccgcggac tacagcaaca cccgcgaact cggccgcgtt
    45421 ctctacaccg attatgtgta tgccttcgaa ctcgcctcgc tcgtgctgtt ggtggcgatg
    45481 gtctccgcag tggcgctgac cttgcgcaag cgcaaggggc tgaagtccat cccgccgtcc
    45541 gagcaggtcg ccgtcaggcg agagggccgg gtcgagctgg tgaagatgca ggccgagaaa
    45601 gacgactgac aacgtacgcg aacaaaacaa gaaccgcgcc ggcggcagcc ggcgcgatca
    45661 gggggtcact gatgctttcg ctttcccatt tcctcatcct gggcgcgatc ctgttcgcga
    45721 tcagcgtggt cgggatcttc ctcaaccgga agaacctgat cgtgctgctg atggccatcg
    45781 agctgatgct gctctcggtc aacatgaact tcgttgcgtt ctcgcattac ctcggcgaca
    45841 tcgccggcca ggtcttcgtt ttcttcatcc tcaccgtggc ggcggccgaa tcggcgatcg
    45901 gcctcgcgat cctgatcgtg atgttccgca acatgcgcac gatccacgtg gatgatctgg
    45961 acagcctcaa gggttaagga agcgtcacga tgtcggacat gaaagcaatc tatctcctcg
    46021 tgccgctggc gccgctggcg ggagcgatcc tcgcaggcct gttcggaaag cagatcgggc
    46081 gtgccggggc gcatgtggtc acgatccttg gtgtggcgat cgccttcgtg gcctcgctct
    46141 tcatctacca ggatgtggcg gccggaaaca ccttcaacgg caccgtatat acctggatgc
    46201 aggcgggtgg catcgacttc gaggtcggtt tcctgatcga ctcgctgacg gtgatgatga
    46261 tgctggtcgt taccttcgtg tcgctgatgg tgcacatcta caccatcggc tacatgtcgg
    46321 aagatccggg ctaccagcgc ttcttcagct acatctcgtt gttcaccttc tcgatgctga
    46381 tgctggtgat gtccaacaac ttcctccagc tcttcttcgg ctgggaggcg gttggcctgg
    46441 tgtcctacct gctgatcggc ttctggtacg agcgtccgac ggcaatctac gccaacatga
    46501 aggccttcct ggtcaaccgt gtgggcgact tcggcttcct gctcggcatc ggcctcgtcg
    46561 ccgcctatgc cggcagcctc gactacgctg aggttttcgc caaggcgaag gaactctccg
    46621 tgaccgagat ggcggtgacc ggctggccgc tgctcaccgc gatctgcatc tgcctgttca
    46681 tcggtgcgat gggcaagtcc gcccaggtgc ctctgcacgt gtggctgccc gactcgatgg
    46741 aaggcccgac cccgatctcc gcgctgatcc acgcggcgac catggtgacc gcgggcattt
    46801 tcatggtggc gcgcatgtcg ccgctgttcg agctgtccga caccgcgctg tccttcgtcc
    46861 tcgtgatcgg tgcgaccacc gcgctcttca tgggcttcct cggcatcgtg cagaacgaca
    46921 tcaaacgcgt cgtggcctac tcgacgctgt cgcagctcgg ctacatgacg gtcgcgctcg
    46981 gcgtctcggc ctactcggcc gcggtcttcc acctgatgac gcacgccttc ttcaaggcgt
    47041 tgctgttcct cggcgccggc tcggtgatca tcggcatgca ccacgatcag gacatgcgca
    47101 acatgggcgg cttgcgcaag tacatgccga tcacctggat cacctcgctc ctgggttcgc
    47161 ttgcgctgat cggttttccg ttcttctcgg gcttctactc caaggactcg attatcgaag
    47221 cggtgcattc ctcgacgatc gcgggctcgg gctatgcgat gttctgcgtg ctcctcgggg
    47281 tgttcatcac cgcgttctac tccttccgga tgtacttcct cgtgttccac ggcgacgagc
    47341 gcttcggcaa ggcgcatgat cacgcacatc acgatcatca cggcgaccac gacgacgagg
    47401 aaccctcggc ggatcatcac cacggcctgg cgccggggca gaagccgcac gagtcgccct
    47461 gggtggtgac cctgccgctg gtgctgctgg cggtgccctc ggtcctgatc ggcttcttca
    47521 ccatcgaccc gatgctgttc ggcgagtggt tcaagggtgt gatcctcgtc ggcgacaacc
    47581 acgtcggcct gcgggagctc gcagccaact accatggccc ggtcggcatg gcgatccacg
    47641 gtctccagac ggcgccgttc tggctggcga tgggcggagt cgcgcttgcc tggttcttct
    47701 acatgaagcg tccggatatc ccggcggcga tccagcgcac cttcaagccg atccacacgc
    47761 tgttggagaa caagtactac ttcgatcgct tcaacgagat cttcttcgcg gcaggtgcgc
    47821 gtctgctcgg acgcggcctg tggaaggcgg gcgatcaggc cctgatcgat ggtgtcgcgg
    47881 tgaacggctc cgcgcgcctg gtgggctggg tggcgcagat gtcgcgcctc ttccagacgg
    47941 gtcacctgta ccagtacgcc ttcatgatga tcatcggcgt gttcgtgctg ctcacctact
    48001 ggttcaaccg gggctgagac gggcacgcag ggacggaaac acataacaag atcgaacgcg
    48061 ccgctcgcgg cgcactgaac ggaaagcaac gatgacggac attcctctcc tcagtctggc
    48121 gatctgggta ccgattatcg gtggcctgct ggtgctcgcg accggctcgg atcgcaacgc
    48181 tccgctggcg cgcatgctcg ccttggcggt cgccgtggct ggcttcgtcg tgacgattcc
    48241 gctctacacg ggcttcgacg tcggaaccag cgcgatgcag ttcgtggaac tgcagtcctg
    48301 ggtgccgcgc ttcaacatca actaccacct cggcgtcgat ggcatctcgg tgctgttcgt
    48361 gctgctcaac gccttcatca ccatcatcgt ggtggcggcg ggctggcagg tgatccagac
    48421 caaggtcgcg cagtacatgg ccgcgttcct gatcatgtcg ggcctgatga acggcatctt
    48481 ctcggcgctc gacgcggtgc tgttctacgt gttcttcgaa gcctcgctga tcccgctcta
    48541 cctgatcatc ggcatctggg gtggcgccaa ccgggtgtac gccgcgatca agttcttcct
    48601 ctacacgctg ttcggttccc tgctgatgct gatcgcgctg ctctacctgt tcatgcagtc
    48661 gggcggcagc ttcagcatcc tcgactggca ccagttgccc ctgccgatcg agccgcagat
    48721 cctcatcttc ctcgccttcc tgatcgcttt tggcgtcaag gtgccgatgt ggccggtgca
    48781 tacttggctg cccgatgccc acgtcgaggc acccaccggc ggatcggttg tacttgccgc
    48841 gatcgccctg aagctcggcg cttatggctt cctgcgcttc tccctgccga tcgtgcccga
    48901 cgcggcgaac gagctcgcgc cgctggtgat caccctgtcg ctgatcgcgg ttgtgtatat
    48961 cggcttcgtc gcgctggtac aggccgacat gaagaagctc gtggcctact cgtcgatctc
    49021 ccacatgggc ttcgtgaccc tgggcttctt catgttcaac agcctcggcg tcgagggcgc
    49081 tctggtgcag atgatctctc acggcttcgt ttcgggcgcg atgttcctgt gcatcggcgt
    49141 gctctacgac cgcctgcact cgcgccagat cgccgactac ggtggcgtgg tgcacaccat
    49201 gccgaagttc gcagccttct tcatgctctt cacgatggcc aactctggcc tgccggccac
    49261 ctccggtttc gtcggcgagt tcatggtggt gcttggggcc gtgcagtaca acttctggat
    49321 cggcttcgtc gccgccacca cgctgatcct cggcgctgcg tattcgctct ggatgtacaa
    49381 gcgggtggtg ttcggcaagg tggccaaccc ccatgtcgcc gagctcgagg acatcaacgg
    49441 ccgcgagttc gccttccttg cggtgctggc ggcgtgcgtg ctggcgatgg gcctgtatcc
    49501 cttccccttc accgaagtga tgcacgcctc ggtcaatgaa ctcctgcggc atgttgccgt
    49561 gagcaagctc tgagcgctgc ccggcgcgag cgcacgagac actttacgga ctggtcagaa
    49621 gatgaatttc gtcgtccccg acttctaccc cgcagcggcc gagatcttcg tggccgtgat
    49681 ggccttggtg atcatgctgg cgagtacctt cgcgcgcagc gtcgctcgca acttggctta
    49741 tctgcttact caggcgaccc tgatcgccgc ggccttcatc acgatcttca cgatggaggg
    49801 ggaggtggtc cataccttca gcaatctgtt catcagcgat ctgctgggtg acgtgctgaa
    49861 gctgatgatc tatttctcca ccgcgatcgc gctgctgtac gggcgtggct acctcgccga
    49921 ccggaagatc gacaagcccg agtactacct gctcgcgctg ctgatgacgc tcggcatgat
    49981 ggtgatggtc acggccaacc acatgctgcc gatgtacatc ggcctcgaga tgatgtcgct
    50041 agcgctgtat acgatggtgg cgttcgatcg cgactcggcg cgctcgaccg aggcggcgat
    50101 gaagtacttc gtactcggcg cgctggcttc gggcctgctg ctgtacggca tgtcgatggt
    50161 gtatggcgcg accggtacgc tcgagttctc gggtgtggcg caggcgctct acaaccagac
    50221 cgccaaccag accgtgctga tgttcggcgt cgtgttcctg gtcgcgggta tctgcttcaa
    50281 actcggcgtt gtgcccttcc acatgtgggt gcccgacgtc tatcagggcg cgcccaccgc
    50341 agtgacgctg gtgatcgcca cggcgcccaa gctcgccgcg ttcgcgatgg cggtgcgcct
    50401 gctgatctgg gcgctgttcg acatcgccga ggaatggcag atgatgctga tgctggtggc
    50461 cgcagcctcg atcgtgctcg gcaatctggc cgcgatcgcg cagcagaaca tcaaacgcat
    50521 gctcgcctac tcgggtatct cgcacatggg cttcatgctg ctcggcctgc tcgcgggcgt
    50581 ggtagagggc gaccgtcact ttgcgctcaa cgcctacagc tcggcgatgt tctacgcgat
    50641 ctcttacgtg atcatgagcc tggcttcgtt cggcatgctg attctgctgt cgcgtgcagg
    50701 tttcgaggcc gagaacatcg acgatttcaa ggggctcaac aagcgcagtt cgtggtacgc
    50761 gctgatgatg ctcttcgtga tgttctcgat ggcgggtgtg cccttcttca tcggcttctt
    50821 cgccaagttg tcggtgctgc aggcggtggt cgcggccggg tacttctggc tggcggtgct
    50881 ggcggtggtg atgtcggtga tcggtgcctt ctactacctg cgcgtggtca aggtgatgta
    50941 tttcgacgag ccggtcgact cctcgccgat tcatgctgcg cccgaagtgc gtgtgatgct
    51001 ctcggccaac ggtcttgcga tcgcggccct cggcctcgcc ccgcaggcgc tgatgtcctt
    51061 gtgcgcgttc gcgctgctgc gttcgctctg atccagcggt gggtgcggac gccagcgtct
    51121 tcctgctgtt ggccatcgcc ttcacgatgg ccaacctgcc cttcgtcttc gaccgcatct
    51181 tcctcatccg caagccgcca gccggggttc gcaaggggct cggctggcgg tttgccgaac
    51241 tgctcgtgct gtatttcgtg gtgggtgggc tcgcagcatc gatcgaggcg cagtcccacg
    51301 gtcgggtgta tgggcagggc tgggcgttct acgcgaccac cttctgtctc tttgtggtgt
    51361 ttgcctttcc gggcttcact tgggcctacc tgtggcgttc ggcgcgccgg acatccgagg
    51421 taggtgcgtg agcgggctcg agcgggacgc gctacatgag tacaccctcg atagcgagtc
    51481 ggttttcgac ggtgcactgc tcaaggtgtg gcgagaccgc gtcgaattgc cggacggcag
    51541 ccgcagcgtg cgcgagtaca tccgccatcc cggcgctgtg gtcgtggtcg cgctggagga
    51601 gagcggggcg ctggtgttcg agcggcagtt ccgctatccg ctgcaacgcg ccttcctcga
    51661 gttgcctgcc gggaaaatcg acgccggcga ggacatcctg gcctgcgcgc agcgcgaact
    51721 gcgcgaggaa accggcttcg ccgctgcgaa ttgggaatat gtcggggtga tgcacccgtg
    51781 tatcggctac tcggacgagc gcatcgaagt cttcttcgcc cacgagctct cgcaggtggg
    51841 gggggcgctc gatgacggcg agtttctcga agtgctgagc ctgcgtgcgg acgaggtgga
    51901 gcaggccatc cggatcggac gcatcaccga cgcgaagacc atcaccgctt attaccttgc
    51961 gttgccgcat atccaggcgc gtctgcgggg acgctagcgc gcttcgccgc cgggtcgcgc
    52021 ttctctcttc actgccggcg cgaaccccgc tggggctccg cggctcctgc tgcgccggtc
    52081 tcgatgccgc cgggtccctt ctcgcgaagg aatgggctgt gctccgccag ttcgtcgaga
    52141 tagtgcccca cgcccgcacg ctcccttgcc agccaatcgt cgaccgcgcc gcgaaagcgc
    52201 ggatccgcga gccagtgcgc ggagaaggta cgcaccggga gcagaccgcg ggcgagcttg
    52261 tgttccccct gtgcgccgcc ctcgaagcgg gtcagaccat gctcgatgca gtattcgatg
    52321 gcctggtagt agcacagctc gaagtgcagg aagggcaggt cctcgacgca gccccagtaa
    52381 cgcccgtaca gcgcctcgcg atcgcacagg aagaaggcgg cggcgatcgg ctgggtgtcg
    52441 cggcgagcca ccagcagacg gacggcctcg ggcatgctgt gcgcgagccg cacaaaaaaa
    52501 tctgctgcga gatagggcgt ggagcggtgc tgcgcgtagg tgttggcgta acagtgcgcg
    52561 aagaacgccc agtcctcgtc ccgcgcttca tgcccgtcca gccactgcag atccagaccg
    52621 tgtgcggctg cgcggcggcg ttcctggcgg atcttcttgc gcttggcgcc ggacaggctg
    52681 gcgaggaaat gctcgaagtc ggcccagcgc cgctgccgga cggagatagg cgcgctggat
    52741 tccgcgggcg tcggctcggt ctcggcggag gcgggtggat ttctccaatg gaactgcacg
    52801 tcctcgcgga tcattagccc ggcggcctcg agcagcgggc gctcgcgatc gagcgggaaa
    52861 agcaggtgca gcgacgacag cccgctctcc agcgccttcg cgagcagggc ggcgagcagt
    52921 tcccggcgtg cgccgtcgtc gtggccgagc aggcgtggcc cggggatggg cgtgaagggc
    52981 acggccgcga gccacttcgg gtagtagtcg aggccgtggc gctggtaggc ctcggcccag
    53041 gcccagtcga agacgtactc gccccgggaa tgcgtcttca catagagcgg catggccgcg
    53101 cccaacgtgc cgccgcgcca cagggtggcg tgcgcgggcg tccagccgct gcgcccgccc
    53161 acgcagccgc tctcttcgag ggcgtgcagg aaggcgtggc ggatacaggg cgcggcgctt
    53221 gcgcagctcg cgtcgaccag ggcatcccac tggcgtgcgt cgatacgggc gatgcggggg
    53281 atgaagtgga atccgtcggg ctggctcatg gtggctgggg cagggggcac ggctgcctgt
    53341 tttttgtgca gtgcgacgat acgttgccgc gacagcgact tgcaagcgct atccacgcct
    53401 tgtccacagg cttgcgcacc cgggctgtgg gtttgtacgc gggcatccac ggtcgcgctt
    53461 cgctcgcgag acggccgcac ggttcgagtc ggggcaaacc atcgcgtagc ggagcgcgct
    53521 tgggcgcgag agcgtgctca ggcgcgcggt ccggcgcacg cagagcctgt cgtggagctt
    53581 cgccgaagcg gtatagttcc gctccatgaa cgcacatgcc caagcccccg tttccatcgc
    53641 tgtcgcccag ctcaacctga ccgtgggcga tctggtcggc aacgccgacc acatcatcgc
    53701 cgcgctcgac gacgcacgcg cggccggcgc gcaactgctg ctcacgcccg agcttgcgct
    53761 gtccgggtat ccgcccgagg acttgctgct gcgaccggat ttctatcgcg cctgcgcgcg
    53821 tgaggtcgaa cgcatcgccg ctgctgcgcg gggggtcacg gtggtgcttg gccacccggt
    53881 ggaggacggc ggcgagcgct acaatgcggc ctcggtgttg cgcgacggcg cggtgctgac
    53941 gcgctatcac aagagcctgc tgcccaacta cgaggtcttc gacgaggagc gctacttcga
    54001 ggccggcggc acggcttgcg tgttcgagtg cgctggcgtg cgcttcgggg tgaacatctg
    54061 cgccgacgtc tgggagcgcg gtcccgcgga ggccgcgcgg gcggccgggg cgcaggttct
    54121 gctggcgctc aacgcttcgc cctaccacat gaacaagcag gctcagcgcc tggaggtgct
    54181 gcgcgcgcgc gtggccgaga ccggcctgcc cgtgctgtac tgcaacatgg tgggcggcca
    54241 ggacgagttg gtcttcgacg gcgcctcctt cgcgctcgac gccggcggcg cggtcgggct
    54301 gcaactcggc agcttcgtcg agcgcgtcgc gatcgtcgac tacgcctcgg gcggttggtc
    54361 gggcggtgag cacgttcctg cgcggacgct cgaggccgag gtgtacgagg cgctgcgcat
    54421 cggggtgcgc gactatctcg ccaagaaccg tttcccgggc gcgatcatcg gcctgtcggg
    54481 cgggatcgac tcggcgctga ccctggcgat cgcggtcgat gcgctcggcg ccgacaaggt
    54541 gcgtgcggtg atgatgcctt cgccctacac cgctcaaatg agcctcgacg actcgcgcga
    54601 catggtggcg cggctgggcg tgcgctatga cgagatcgcg atcgagccgg cgatgaaggt
    54661 ctttgccgag ctgcttgccg accagtttgc cggcctgccc gccgacacca ccgaggagaa
    54721 cctgcaggcg cgcatccgcg gcatgctgct gatggcgctg tcgaacaaga ccggcgcgat
    54781 cgtgctcacc accggcaaca agagcgagat ggccaccggc tacgccacgc tctacggcga
    54841 catggccggc ggcttcgccg tgctcaagga cctctacaag acctttgtct tccgcctgtc
    54901 gaactggcgc aacacggtga gcccggtgat cccgcagaac atcatcgacc gaccgccctc
    54961 ggccgagctc aagcccgacc agaaggacca ggacagcctg ccgccctacg acatgcttga
    55021 cgccatcatc caggcctaca tggagcgcga cgagtcgccg cgcgacatcg tcgccgcggg
    55081 cttccccgag ggcgaggtgc ggcgcgtggt gggtatgctc aagcgcaacg agtacaagcg
    55141 ccgccaggcc ccggtgggca tccgtgtgac ccagcgcggt ttcggcaggg attggcgtta
    55201 tccgataaca tcccgctacc aggacgaatt ctgaaagagg agcaagccgg atgaagaaga
    55261 tcgaggcgat catcaagccg ttcaagctgg acgaggtgcg cgagggcctg tccgaggtgg
    55321 ggattacggg cctgacggtg accgaggtca agggtttcgg ccgccagaag ggccacaccg
    55381 agctctatcg cggtgctgaa tacgtggtcg acttcctgcc caagatcaag gtcgaggtcg
    55441 tgcttgccga tgacatcgtc gaccaggcgg tggaggcgat cgtgaaggcc gcgcacacgg
    55501 gcaagatcgg cgacggcaag atcttcatca cgccggtgga gcaggtcatc cgcatccgga
    55561 ctggcgagac caacgaagcg gcgatctgat attcgggcgg cggtactcag gcgggtaacg
    55621 gcattcgggc gcgcggcgcg gcgttctgcg cgcgcagcag caccagcagg gcgccgccgc
    55681 cgccatcgtg gggaccggcc tggcaaaagg cgaggatctc ctcgcgctgc gccagccagc
    55741 cgcgcgacag ctgtttcagg atcgacacct tcccgggcga gccgtggccc ttgccgtgga
    55801 tcacccggat gcagcgcttg ccctgggcaa gcgcgtcgtg caggaagttc gccagcgcgg
    55861 cgcgcgcctc gtcgcgggtg aggccgtgga ggtcgatctg gccctgcagc acccagcgcc
    55921 cgcggcgcag atcggtcagc acccggcgcg gcagcccggt ccgcaggaag gccgcctcgt
    55981 cgcccatgtc caggcgatcc tcgaaggtga gcggtgcggc gagcgattcg ccgagcgccg
    56041 cgcgctcgtc ctcctcgcgc ttgagcggcg caggtgccgg ctgcgggcgc tccagctcca
    56101 cgcggttgcg gtcggtgacc gggcgggcat tgcccaccac cgcaagaaag agcgctgccg
    56161 gatcgctgac gtcggcgtcc gcgggcagca cgatcgcgtc gggtcgcagc gccggcgcgg
    56221 gatgcgtacc gcctgcatgg cgggcgtggt gacgcagcgg tgtgtcgagc gccactcggc
    56281 cggtatccct cagcggcatc acgccctcgt aggccgcgcg tagcgggtcg gtctcgggtc
    56341 gcgcacggac gggctcctcg gtttcctcgt cctccacggc ttgcgtgcgc ggccgcgggg
    56401 cagggcgggg gcggtggatc tcgacgcggt ccgtgccgcg caccgggcgc accgcgccga
    56461 cgctgcgccg gaagagctcg agcgcctccg cgtcgggctc ctgtgcacga tcggcaccgg
    56521 cgctggagga gtcttcctca agcgggtggc ggatacgcct cgccggggcg ggcgacgaca
    56581 cgggtgccgg gagcgcgcgc tgctgcagct gcttgcgcag ggctgcgaag ggcgaggcgg
    56641 gcttcggcac cgcgccctgc gcgtcgcgtc ctgggttgcc ggccggaccc ggcgcgggag
    56701 cgcggcggct catcggcctg ctcagacgcc gagggcgtcg aggtagcgct cggcgtcgag
    56761 tgcggccatg cagccggtgc cggccgaagt caccgcctgg cggtaaatgt ggtcctgcac
    56821 gtcgccggca gcgaacacgc cgggaacgct tgtctgcgtc gcgttgccgt tgcgcccgcc
    56881 ctgggtgacg atgtagccgc cctccatctc gagctggcct tcgaaaatgt cggtgttggg
    56941 cttgtggccg atggcgatga acacgccctg cagcgccacc tcccgggtgg cgccgttgtt
    57001 cacgcccttg atgcgcatcc ccgtcacgcc ggtgttgtcg cccagcacct cgtcgagcgt
    57061 ggcattgagc gcgagctcga tcctgccggc ggcgaccttc tccatcagct tgtcgatcat
    57121 gatcttctcg gcgcggaact tctcgcggcg gtgcaccagg gtgacctttc tcgcgatgtt
    57181 ggcgaggtag agcgcctcct cgaccgcggt gttgccgccg ccgatcaccg cgacgtcctg
    57241 gttgcggtag aagaagccgt cgcaggtggc gcacgccgac acgccgcgcc cggcgaattt
    57301 ttcctccgag ggcaggccga ggtacttggc ggtggcgccg gtggagatga tcagcgcatc
    57361 gcaggtgtat tcgccgctgt cgccgatcag gcggaagggc ttctcgccca gcttcacggt
    57421 gtggatgtgg tcgaagatca tctcggtgtt gaagcgctcg gcgtgcctct ggaagcgcgc
    57481 catcaggtcc ggacccatca cgccgtctgc atcggccggc cagttgtcga cctcggtcgt
    57541 ggtcatgagc tggccgccct gggcgaggcc ggtgatcagc accgggttca ggttggcgcg
    57601 cgcggcatag acggcggcgg tgtagccggc ggggccggag ccgaggatca gcagacgggc
    57661 gtgtttggtg gtcatggacg aggcttcctg cgggtggatt gcgaggcgcc gattatagcg
    57721 gagggggcgg cgggggttgg aggtggtcgc cggagtccgg gcgctcgcgc gtgattccga
    57781 tggggagacg cgcgttcctg tctgtggtgc agcgacacgc atcgaattcc cgctggatgc
    57841 ggaaagtgtg atctccgtca cgaaatcgag aataagcccg gggtgatcgt ataaacttga
    57901 aaatgcatgt ttcaactgtt ccactcctcg tgcctggagc gcgcatgacc caagccaccg
    57961 ccgtttccgt cgtcgccctg aagaccttcc cgctgttcca tggcctgtcc gacgagtcgt
    58021 tggccagcat cgcgcgcgtg tcgatgatgc gtcgcttccc ccgcgggcag tccgtggtgt
    58081 gcgcgggcga ccgtcccgac tacgtctatc tcgtgctcac cggcagcctc aaggtcgtgg
    58141 tgagcgacga ggacggccgc gaggtaatcc tgtccatcct cggccagggc gagctcttcg
    58201 gcgagatggg catgttcgac gagcggccgc gctcggcctc ggtgatcgcc gtggtgccgg
    58261 ccgatctggt gctgatctcc aagcaggatt tccgccgcat catgcaggac agcttcgacg
    58321 tgtcctggcg catcatgtgc aacctcgccg agcgcctgcg caacgccgat cgcaagatcg
    58381 agagcctcgc gctcatggat gtctacggcc gtgttgcgcg cctgctgatc gagatggccg
    58441 aggacgtcgg tggccagtcg gtggtggtcc ggcgcatctc caagcaggac atcgccaaga
    58501 tgatcggcgc ctcgcgcgag atggtgagcc gggtgatgaa ggacctcggc cagcagggtc
    58561 tgatcgagga agggcccgac ggcatcctgt tgcgtgagcg cctcaacgac gtgtgaacgc
    58621 gcgctcgccg ctcggccgtt tgtgagcatc cgcaagcgcg gcggcggggt ggggaccgat
    58681 cccgtataat ccggggttgt tccactctcc ctgcggccgc aacgcaatgc tgtctcgttc
    58741 gtcctcccgt tcccagccgc tgcccgaaaa gatctcgctg ttgctgcagg aggcccgctg
    58801 gctgatcctc ggcgtggtct cgctctacgt cggcatgatc ctcgtcggct acagcgagtc
    58861 cgacccaggc tggtcgcacg cctccgacgt cacccgcgtt gccaatccgg gcgggcgttt
    58921 cggcgcttgg ctggcggacc tcctgctcta tctcttcggc atttcctcgt ggtggtgggt
    58981 ggtcttcctc ggctacagcc tgctgtgggg tttccgccgc ctcaagaaca ggctcgagct
    59041 cgaccaccgc tccttcatct tcgtgatgat cggcttcttc gtggtgctga tcaccagcag
    59101 cgcgctcgaa ttcctgcgtt tccactccca cggtgcggcg ctgccgctcg agcccggcgg
    59161 cttgatcggc atggaactcg cccagttcgt gcggcgcagc ttcggcttca ccggcggcac
    59221 gctgctgctg ctcgcgctgc tggccacggg gctgtcgctg ttcaccgggg tgtcgtggct
    59281 ggctctgatg gagcgcatcg gtcttggcat cgagcaggcg gtgctcggcg tgcagcaggc
    59341 ctggctgcgc tggcaggacc gccgtgtggg tcgcgaggtg gcgcacaagc gcgaggaggt
    59401 ggtgcagacc cggcgcaaga aggtcgagaa ggccgagccc gcgccgctgc gcatcgagcc
    59461 cgcggtggtt gccgtgccca agtccgagcg ggtggaaaag gagcgccagc agaccctgtt
    59521 ccaggatgtg cccgagggcg tgatcccgcc ggtggcactg ctcgatccgg ccagtggtgg
    59581 cgtggagccg ccttcgcccg aatccctcga gttcacctcg cggctgatcg agaccaagct
    59641 cgccgacttc ggcgtcgagg tcaaggtgct cgccgcctac cccggcccgg tgatcactcg
    59701 ctacgagatc gagccggcaa ccggggtcaa gggcagccag gtggtgaacc ttgccaagga
    59761 cctcgcgcgc gcgctgtcgc tggtgtcgat ccgcgtcgtc gagaccgtgc cgggcaagtc
    59821 ctgcatggcg ctcgagctgc ccaacccgaa gcggcagatg gtgcgcctgt cggagatcat
    59881 cggttccaag gtgtaccagg acgcgcattc gccgcttacc gtggtgctcg gcaaggacat
    59941 cggtggccag ccggtggtgg ccgacctggc caagatgcca cacctgctgg tggcgggtac
    60001 gacgggctcg ggcaaatcgg tcggcatcaa cgcgatgatc ctgtcgctgc tctacaagag
    60061 tgagcccgat cgggtgcgct tgatcatggt cgacccgaag atgctcgagc tgtcgatcta
    60121 cgagggcatc ccgcacctgc tcgcacccgt cgtcaccgac atgaagcacg ccggcaacgc
    60181 gctcaactgg tgcgtggcgg agatggacaa gcgctacaag ctgatggcgg cggtgggcgt
    60241 gcgcaacctc gccggcttca acaaggccgt gcaggaggcg cgcaaggccg agcagccgct
    60301 caccaaccca ttctcgatca gccccgagaa cccggagccg ctcgagccct tgccctacat
    60361 cgtggtggtg gtcgacgagc ttgccgacat gatgatggtg gtcggcaaga aggtcgagga
    60421 gctgatcgcc cgcctggcgc agaaggcgcg cgccgccggc atccacctca tcctcgccac
    60481 ccagcgccct tcggttgacg tgatcaccgg cctgatcaag gccaacgtac ccacgcgtat
    60541 cgctttccag gtctccagca agatcgactc gcgcaccatc ctcgaccaga tgggcgccga
    60601 gaccctgctc g
//
LOCUS       DAOQBS010000004        53212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_269_length_53212_cov_16.291307, whole genome shotgun sequence.
ACCESSION   DAOQBS010000004 DAOQBS010000000
VERSION     DAOQBS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 53212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53212
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_269_length_53212_cov_16.291307"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="PKN25_00890"
     CDS             <1..1388
                     /locus_tag="PKN25_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNV89479.1"
                     /translation="AAKALARFMRLHPHNIGQKTEVMVEHFQHFTRHKIGGHAKAMVV
                     TGSRLEAVRYKQEFDRYIQEKGYPIKSLVAFSGSVEDDRIAEKTYTEVEMNGGLKEKA
                     LPETFAKPDFRVLLVAEKYQTGFDQPLLHTMYVDKRLAGIQAVQTLSRLNRTHPLKDD
                     TFVLDFVNEPGEIQEAFRQYYEGAVMGEEVDPDKLYAVKAELDASGIYRQTEVSDFSR
                     VFFAPKRRQSPGDHKAMNAILDQAVARFVQLQNTEEEEAELWRGKLQAFRNLYGFLSQ
                     VIPYQDSDLEKLFTYLRHLALKLPKRKSGPGYQFDEEVELDYYRLQKISEGSISLDEG
                     YAKPLDGPREVGSGMVREEHVSLSRLIDIINQRFGGELNEADQLFFDQIAEAASLNES
                     LQRAAEVNSLDKFQLVFRQVLESLFIERMELNEELFTDYMAKPEMQALVSKWLGGQVY
                     DRLSAARRRVS"
     gene            complement(1439..2743)
                     /locus_tag="PKN25_00895"
     CDS             complement(1439..2743)
                     /locus_tag="PKN25_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV89480.1"
                     /translation="MNARQSGLPHPFIRTKPGAEAVASPTSAAPAPAFETLAARFRPI
                     FARIAERAAEREHDRELPFDAVGWLREARFGALRVPVEHGGFGASVEQFFALLIELAE
                     ADSNLPNIFRAHFGQIERLYAEIDPALHAPWLERAAAGEIFGNATTEIGDGALGQPAT
                     RITKQEGVLRLNGTKYYSSGSLYADWIAVSAQLQEEGVAGGAEQPVIALVRADAPGVE
                     RIDDWKGFGQRLTGSGTTRLTDVEVDPASIWPCARSGPTSMTAFFQLVHLATLTGIAR
                     AIERDACAYVRPRKRVFSHGSGAIPREDPLIQQVVGQLASTAFIAEAAVRAVADRLGD
                     IDRLRRRGEAVPESLLVDVELAAAKAQVGLVDAVLTSTTRLFDVGGASAVQENRRLDR
                     HWRNARTLASHNPVIFKARVVGDHAINGTAPGFYWTVGAATG"
     gene            complement(2740..4125)
                     /locus_tag="PKN25_00900"
     CDS             complement(2740..4125)
                     /locus_tag="PKN25_00900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNV89481.1"
                     /translation="MPALAFNAFVMNTASHIQHGQWRHPDARQVDFNDVDLWVDLART
                     LERGLFDAIFFADVVGLYGPLGGDYEVNAREGLQIPSNDPSVLLSALAVNTEHLGLAF
                     TSSVLQAHPFDFARKVSTLDHISRGRVAWNIVTTHLENAARNFGQDQLTPHDERYAWA
                     AEYVDVVYKLWEGSWDEGALLIDRARGVFADHNKIHKIHHVGPRYRVEGPHLSAPSPQ
                     RTPLLFQAGSSGAGRQFAAANAEGQFIVTPNPTVAAELIRDTRRLAVATGRRAEDIKF
                     FQGLSFIIGSTEAEVRRKEAEYDEYASVDGFLAHHNYAVDRYGKPYPADTPLAAIQSE
                     ASQSILDWYAKSSPGTEPTVADLGRLASRRGRVAGTPEQIADQLELWQQAGVDGINVI
                     NWHLPGSYEEFNEHLLPVLQQRGLAKREYRPGTLRQKLFGSDRINERHPAARYRGAFT
                     PKTETRNVEFA"
     gene            complement(4182..5459)
                     /locus_tag="PKN25_00905"
     CDS             complement(4182..5459)
                     /locus_tag="PKN25_00905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV89482.1"
                     /translation="MPIEDPTLRNAREAELATYANDIAAAIRPTAARYDRTGEFPFEH
                     FEILRHKGALGLTVPEEYGGRGLSLYETLLVQERIAGASGATALALGWHLMTFFGLST
                     TRAWDEAVFERVCRDAVERGELINVFATERDAGNVVRGFKPTTVARRHGDGYLISGRK
                     AFSTLAPAVNHFTVLAHIEDDDQTAEFLVRKSARVRIVETWDSLGMRSTGSHDVELDA
                     VEVPADALLVRLEKGKPSRFMACSGAYSLQLPAIYLGIAVAARDFILDFADSRFAPSL
                     GGVILDAPQVQHKIGEIEILIGVSKSLLYGLAERWDRHDDLKDKLGSEVAITKYAVTN
                     NAIRIVELAMQIAGGHALSRDLPLERYFRDVQCGLYNPPHNDMVIDRVAREAIARQRA
                     DRNPSAVIPITPTPQGPDRTAHTATTRSSEAIA"
     gene            complement(5480..6688)
                     /locus_tag="PKN25_00910"
     CDS             complement(5480..6688)
                     /locus_tag="PKN25_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV89483.1"
                     /translation="MSLSDAHTLEPNTVSAESPAAIAARLAARFAETAAARDLAGGTP
                     KAERDLLRASGLLKLSIPRAYGGLGASWAETLSVVRQLARADSSVAHVFAFHHLQLAS
                     VRLFGSPEQWQPWLLDTVTQGWFWGNALNPLDKRTVATRVDGGREFSGRKSFCSGALD
                     SDMLLASAVEDSAEGKLIIAALPTRRAGITLLEDWDNMGQRQTDSGSALFERVAVAED
                     EILSRPGPLSSPHACLRPLLAQLIFVNIFLGIAEGALAEARHYTRNEARLWSASPAAH
                     VSEDPYVLAHYGEFWVALESTRALADRAAARFDAAWDTGLALSTEGRGEVALAVATAK
                     VAATRGGLDLTSRIFEVTGARSTTAALRLDRFWRNLRVHTLHDPVDYKVRELGDWALN
                     GRYPKPSFYS"
     gene            complement(6782..7933)
                     /locus_tag="PKN25_00915"
     CDS             complement(6782..7933)
                     /locus_tag="PKN25_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV89484.1"
                     /translation="MIRCQEFRGLAPAQECNMPESNTAKLLTLPNAGQGALSIRAKAL
                     IFHDAKSLRLLRQVELIAPTEANVLIIGETGTGKELVARHIHAQSGRSGPFVAVNCGA
                     FSDNLVEAELFGHEAGAFTGAQQARAGWFEAANGGTLFLDEVGDLPLAMQVKLLRVLQ
                     ERQVVRLGSRKPIPVDVRLVAATNVNLEKAVQAGHFRLDLYYRLNVAPVELPPLRERP
                     GDILPLVEYFIDFYRQRLDIPTPRLSQEAAAELVAYHWPGNIRELENVVHFALIVCQN
                     GVVNPGDLRLPGFALSPPGATPDPGGSHADGFATVRAGLRRLLDEDTPEVYERIEQLL
                     FTTAFEYCRENQVRTAKQLGISRNILRTQLKRFGLIGDTAADALAAADA"
     gene            complement(7971..8663)
                     /locus_tag="PKN25_00920"
     CDS             complement(7971..8663)
                     /locus_tag="PKN25_00920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV89485.1"
                     /translation="MRQTVLIVPGYRGSGPGHWQSWLEGELPEARRVGGIDWDTPLLA
                     RWAGEVRREIDASAGAVWIVAHSFGCLASVVAAVDRPQKVAGLLLVAPADPERFELLG
                     VREDPQRSAGVGRFLPQSGLPCPSFMVVSRSDPWLSQDVAHEWAERWGSRLIDAGDAG
                     HINVESGHGPWPLAPSLLQQLRDSAGDLPLGMITPDQRAPLRGRSGALARLRHQTRRF
                     LSLRRTTMPSTP"
     gene            complement(8671..8886)
                     /locus_tag="PKN25_00925"
     CDS             complement(8671..8886)
                     /locus_tag="PKN25_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNV89486.1"
                     /translation="MAIQAINVRNQFRGRVKEIIRGEVVSEVDVETSSGIVTSVITTR
                     SVDELGLKPGSEVVALVKSTEVSIATL"
     gene            complement(8902..9909)
                     /locus_tag="PKN25_00930"
     CDS             complement(8902..9909)
                     /locus_tag="PKN25_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV89487.1"
                     /translation="MKLGPLRSTLLTLALATGVAGVSHAQTTLLNVSYDPTRELYQDF
                     NPEFAKHWKARTGETVTIKQSHGGAGKQARAVIDGLEADVVTLALAYDIDAIAEQTGK
                     IPADWQKRLANNSSPYTSTIVFLVRKGNPKGIKDWGDLVKPGVEVVTPNPKTSGGARW
                     NYLAAWAYALKQPGGNEQTAQAFVTELIKHVPVLDSGARGATNTFVQRGIGDVLLAWE
                     NEAFLSINELGPDKFEIVVPSISIRAEPPVTVVDGVAKKRGTEKLAQAYLEYLYSPVG
                     QKLAAKHYYRPVRPEHADPADVARFPKVELITIEDLGGWQAAQKKHFADGGVFDQIYA
                     K"
     gene            10172..10918
                     /gene="ydfG"
                     /locus_tag="PKN25_00935"
     CDS             10172..10918
                     /gene="ydfG"
                     /locus_tag="PKN25_00935"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HNV89488.1"
                     /translation="MIVFVTGASAGFGAAIARSFVKGGHRVVATARRKDRLDALAAEL
                     GDALLALELDVRDADAVAAVPGVLPDGFAEVDVLVNNAGLALGLEPAHKASLEDWHTM
                     IDTNCTGLVQVTRAFLPGMVARNRGHIFNLGSVAGRWPYAGGNVYGATKAFVRQFSLN
                     LRADLLGTALRVTDIEPGLCGGTEFSNVRFHGDDDKAAKVYADVQPLTAEDIADSVYW
                     IATRPAHVNINTIELMPVAQSFAGLSVHRG"
     gene            11191..14235
                     /locus_tag="PKN25_00940"
     CDS             11191..14235
                     /locus_tag="PKN25_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNV89489.1"
                     /translation="MPSVGRRPRPWRVLGVAICVALLVASVAGGLLLWHEAGTSRLQA
                     RALARYAARLDYALLAGPSAAIRFPAHGPFDQRLGYTELARFSERLQARGFALVEQVR
                     FDDALLAHVERGLFPPYAEKTRAGLDVFDCRGEPLYGFRYPWRGYERFEDVPAIVAQA
                     LLFIENRDLLDESRPTLNPAVDWARFARAALGQLGRMVDADLDAPGGSTLATQIEKYR
                     HSPGGITQDAREKLRQMVSASVRAYREGEQTLPLRRRLVLDYLNTVPLSAAPGHGEVN
                     GLGDGLWVWFGAEFARVNALLAAPVGEGEERIAQGRALRQVVALMIAHRRPSWYLAGG
                     REELARTTDAYLRLFAEAGLIGAVLRDAALARPLAFRDLVADPAWVPATPGKGTTAVR
                     TRLAGLLDTALYSLDRLDAELGTTLHGGLQQAVGDYLGQLSDPAFARSQGLIGERMLQ
                     PATLGAVRYSFTLVERTAGGNRVRVQTDTTDQPLDINEGSKLELGSTAKLRVLATYLE
                     LVAELHGRLAALDPPALRRVEVDAQDRLTRWAIDHLASAPDRSLPAMLEAALERRFSA
                     SPGEGFFTGGGRHTFGNFNASDNARNPTLREALQASINLPFVRLMRELVRHTMYQVPG
                     STARLLEDNEDPRRVEYLVRFADREGQLFLRRFWRKYQDSTAEEIRSTLLDGLRPGAD
                     RLAAVFRYLEPEAPPHALADFLARRLGEPPAAARLAQLYARNAPEAFDLPDRGYVARV
                     HPLELWLVAFRLQHPEATLAEAVAASADERQQVYRWLFRTRAKGAQDSRIFTLLEVEA
                     FLEIHRRWARLGYPFGHLVPSLATALGSSGDRPAALAELMGIIVNDGQRLPVQRIDTV
                     RFARATPYESAFAIRPVEGDPVMAPEVARALRGALSDVVEGGTARRLAGAFKLADGSE
                     LALGGKTGTGDNRIVVRGRAGLALNRTATFVFYLGPRHFGTLTAYVIGPEAASYRFTS
                     GLPVQILKSMGPLLIPHLEPAGAHACLPPPLSEAAPQPVTAG"
     gene            complement(14257..16056)
                     /locus_tag="PKN25_00945"
     CDS             complement(14257..16056)
                     /locus_tag="PKN25_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNV89490.1"
                     /translation="MPLPELHAPLFVGLVLVVLFVAFVREWVKPDVAVMVAVGVLLVA
                     GMLNTKEVLGVFGNSAPITIACLFIISAALSRTGCVDQLGEWLAAAAGAGERRLLLAL
                     IAACLLVSPFINNTPVVMVMIPAVIAVAARQGLAPSRLLIPLSYATILGGLITMVGTS
                     TNILVDGVAREQGLAPFSMFEISLPAILMALVGSAFMLVFAPRLLPVRETLSQQFGAG
                     ERWFMTELFVPEGSHLAGRTLREARLSNGIIQVLNLVRGDIELADPAPDTRLEVGDRV
                     VVHSRSDAMMALRSSDGVGLQAPAGAEAHDIETLRRRDLVMVEAIVGQTSRYILRPIR
                     DLDLLARYGIHLVAVHRRDASFAQIGEDFQLLGGDVLLVEGTPAQIQRFCDNGDLFAI
                     TEGRQMASRRHKAPVALATIVGVMLLAALNVMPIEGLALIGAAIVIATGCITSDEAYK
                     SIEWPILVLIFGMLAISIAMRNSGLDQLLAAQLAALGEGMSPWLMLSLVILITSVATE
                     VLSNNAIAVLFTPVVIGLAQQMGVDPRPFVVGVMFAASCSFATPIGYQTNTLVYSAGN
                     YRFADFARLGVPMNVLTWLLCSALIPLFWPFQP"
     gene            16304..17848
                     /locus_tag="PKN25_00950"
     CDS             16304..17848
                     /locus_tag="PKN25_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HNV89491.1"
                     /translation="MRLRFFLSSPGDVADERTFAQQVIEQELPKDPFMRGRVGCEAVR
                     WDDPAAPVAMPATLTPQEAVNRGLPRPSACDCVIVVLWSRLGTPLPASCTRPDGSRYL
                     SGTEWEYEDAIAARPQPHILVYRRRSPPELGDPLDADYDERLAQYRRANDFFKRFRNA
                     DGSLAGGFAEYDTPQEFRDRLRVDLRAFIQRRLDSEQANGQTVRVNAPPPYGRIAKAL
                     ASGSAVPIIGPGICRSGRPGDEPWDPDAPRFLPSGNELSACLADEAGFPSQAERDQLA
                     EVASYYEAFKTRDALRERLRSLLAPAAPDKLAVPPLYSLLASLEKPLLIITTNYDTLI
                     EQVFRNAQKPYDLVVYSELKELGNAVLWWPHGALKPETVAPNELSIELEKKTVIFKMH
                     GSIVPQTSDLDTFVITESDYVELLARIASRSAIPALFASHCQDRNLLFLGYSLRDWSF
                     RTILQSLNRFFAKRLVAGDDEIQSWAIDDRFSELELKFWQRRGVYPYEVNLDEFVRLL
                     RARMSP"
     gene            17845..21396
                     /locus_tag="PKN25_00955"
     CDS             17845..21396
                     /locus_tag="PKN25_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89492.1"
                     /translation="MNAEVRIEQRCPYVGLQPFEEADRAFFFGRERDQRVIIANLLSS
                     PLTILYGSSGVGKSSVLMAGVVPQLHRERPRTPVVVFRNWVDRDFQLALTRACIETVW
                     AMQVDQPRPAETLPLDEVLRACAEAAHGTILVIFDQFEEYFLYHAKDSEAAGAFEAQF
                     ARAVNREDVDVGFLVALRDDSLSRLDRFQERIHNLLSNRLRLNHLEPEAAGDAIRKPL
                     EVWNALQAVGVPPVGIDDELVDELLRQVRIGQVSAGRPGGSGGLRHDEGCIEAPFLQL
                     VMVRLWEEERAQGSDRLRRETLDRLKGADEIVRTHLDVAMARLDATSQAVCASFFDRL
                     VTPSGSKIACGAADLISWAGDLAPHVPAVLQSLSDARNRILRTVSVPDNPEVMRYEIY
                     HDVLAPAILDWRQRFEADRQREEALRVAREKAARREEEAQIARERAARRALHRWLVGL
                     GMMSCVAGAGWLYASWEGMRSAANEKAAQSISNAPIDPGSALSLALEAVEATVPSWMS
                     VARAARLHLTPTAAAEDALRQAIQASRLVWTLPVSGRALSDVAFSPDGGRLLTAGQDQ
                     TARVWDIRSGRPAEPVVTFAHDAWVRRGRFLPDGERVITVSGGLAYLWSLAQPTAPLR
                     SFEHGGNIYSAFDTSADGRRLATAGDGAQKARVIRIWDVDADAPEPLLTLDAEGAWIM
                     GLAFSPDGCCVATAAVERGKAERSAIALWGLERRREILSLPLEEPSDAIAFAPDGSAL
                     VTASRDGWLRVWEPAGKGFEVLLREAAGGTHRGEVLAWNGRTLAGHGDRIRNVEVSAD
                     GALIASAGGDGTARVWSRHTGENLLTLTGHLSYVEAARFSPDGRHVATASRDGTVKFW
                     NIEGHANTVTSIAFSPDGRTLATGSSDRTARIWDFADGTPRLRHTLRGHADQVHRVAF
                     DPSGARLATAGLDNQLRLWDVASGEAVTLPSVPPEHRHKDQLRGVAFSPDGRLLASAG
                     ADGHARLYPLAGEGSPVSVLHGGATIQVSGVAFHPGGERWATSGFDGKLKLWDLSGTD
                     LGTVGLPDDERKLGIRIAGLAFSPDGQYVAAVVQRRVRVWPVAAFKEPEVHPPVRLPL
                     IEGARHCAALAYSPDGRWLAVGCSDAGVRLFEMPAGTLVKTITVHKNEVRGVAFSPDG
                     TLLATASNDKTFHVSPLAFEAVYEAARRLQQATAAER"
     gene            complement(21439..22371)
                     /locus_tag="PKN25_00960"
     CDS             complement(21439..22371)
                     /locus_tag="PKN25_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV89493.1"
                     /translation="MRLTFHQLRLLLAVSREGSVSRAAQRLHLTQPTLSAQLRQLAEQ
                     VGLPLFERVGRRLHLTAAGRVVLDTANRVEQELERLDETLAELRGDVVGRLRLAVVST
                     AETFIPRLLGDFRRERPAVEVSLAVLNRDAVIRRLADNLDDLYIMSRPPQAPPVVATP
                     FLSNPLVVVASTAHPLAGCRRKLEIEAIAAEEFVLREPGSGTRQAAEQFFAAHGLALR
                     PRLELGSNEAVKQAVAGGLGLSLLSAHALAHAVDEGIAVLRVEGTPLPTQWQVVYPAG
                     KRLTPLAGAFLEFLRERAAELEHSAQARLDAARR"
     gene            22561..23502
                     /locus_tag="PKN25_00965"
     CDS             22561..23502
                     /locus_tag="PKN25_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HNV89494.1"
                     /translation="MVDAVPFFFALGAVASLARSGLRLPAAVFETLSIYLLLAIGLKG
                     GVEIARSESATLWVDALLAIGLGALIPLLVFPLFRLRFSRPDAASLSAHYGSVSIVTF
                     AVGAAVLAGRGIEVEGHLALLAALMEAPALIVATLIARWGVKSADGSSTAGALVHEVF
                     ANKSVVLLGGGILIGWIAGPQGLEPLSPLFVDLFKGALCLFLLEMGLIAADRLPDLKK
                     AGLFLVGCALALPPLLALAGWGVAQAMGLGVGGTVLMMTLAGSASYIAAPTAMRIAVP
                     EANPALGVAAALAVTFPFNLLLGIPLYLWWAQAMAGA"
     gene            23869..24348
                     /locus_tag="PKN25_00970"
     CDS             23869..24348
                     /locus_tag="PKN25_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HNV89495.1"
                     /translation="MSTKPKLRSFPDRLRQIAMFEIGGLVLITPPFAWASGVPIRDSI
                     GLLALLALMAAIWNGAYNTAFDWIEGRLTGRTADRRPFRLRAVHALGFESGLLVMTLP
                     VIVWWTGMGWVEALIADIGLAITYTIYAFLFNLGYDRLFPIAPAGGEADAAGLAAER"
     gene            24350..25399
                     /gene="murB"
                     /locus_tag="PKN25_00975"
     CDS             24350..25399
                     /gene="murB"
                     /locus_tag="PKN25_00975"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNV89496.1"
                     /translation="MATGDSAGDVAEAAVVLDADLSALNTFGLRAHAARLLRVRSVAA
                     LQAAIAAPGWSDTARLVLGGGSNLVLGGDFAGTVLRVEIPGRRLLRAEADAWIVEGGG
                     GESWHDFVRWTLAQGWPGLENLSLIPGTVGAAPIQNIGAYGVELVERFESLDAVELDT
                     GELRRFDAAACAFGYRDSVFKRTPGRWLVVAVRFRLPRPWRALTRYADVARELEARAL
                     AQPSALEVSDAVIAIRRRKLPDPAVLGNAGSFFKNPVVDAARCARLLAAHPALPHYPQ
                     ADGSEKLAAGWLIEQAGWKGRDLGPVGCYERQALVLVNRGGATGADVRRIAQAIIADV
                     EARFGVRLEPEPVFI"
     gene            25545..25670
                     /locus_tag="PKN25_00980"
     CDS             25545..25670
                     /locus_tag="PKN25_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNV89497.1"
                     /translation="MRAKLERGWLTYSRTPAEIAPMRTLKAALDPRRILNPGKML"
     gene            complement(25691..26437)
                     /locus_tag="PKN25_00985"
     CDS             complement(25691..26437)
                     /locus_tag="PKN25_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNV89498.1"
                     /translation="MSEDTGNEDLQRDELAEEHEEDGAQRGYTFEDMRLAVGDRLQIE
                     CAASTGVRRAFVRVVGYVDGISLLVTTPVAGGRRVELVENDPVIVRVFSRQSAFAFRA
                     SVLRACRLPLDYVHLSFPSVIQGSVIRKSTRVRTQFPVQVNTQAGGAAGEGTMLNLSA
                     TGALIQTHASLGEPGAIVRLRFDVDLHGLQTELDIEAEIRNVQQAADAEAEGIEYHYG
                     VDFHELGAEDRMKIKSLVYQTIIEQPRRII"
     gene            26664..27551
                     /locus_tag="PKN25_00990"
     CDS             26664..27551
                     /locus_tag="PKN25_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89499.1"
                     /translation="MTTTRRIAPLALACALLAAPAASQAATWSDTFVGYRYGSDFREP
                     TNTKDVEKHVLQFTHASGYSVGQNFLNLDIFQSDKADPANGGGSGATEFYLTYRHQVH
                     LGKAFDRSFAFGPVKEVAVTAGFDLNTKNTAFSPRKRLVVLGPTLKFDVPGFLDVSLL
                     LGKEWNRCGLDPVAPSSFDPCPQTEVSFDPQWIVSAAWGIPFSAGAVPLKFQGFININ
                     GEKGKDYAGIKTHTETLMRTSLMVDVGQMAWGKKNTFLMGVGYEYWRNKFGNHAFANG
                     AEKPGIDTDAPTFQMEWHF"
     assembly_gap    27672..27771
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(27931..29367)
                     /locus_tag="PKN25_00995"
     CDS             complement(27931..29367)
                     /locus_tag="PKN25_00995"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNV89500.1"
                     /translation="MTYPHTRLLIDGQWCDALDGRTLAVHNPATGEEIGRVAHAAIAD
                     LDLAVAAAVKGFQTWRRTPAIERAKTLRRAAALMRERAGDIARVLTQEQGKPLPEAKM
                     ETLAAADIIEWFADEGLRVYGRIVPGRNLAATQMVIKDPVGPVAAFTPWNFPINQVVR
                     KAAAALATGCSILVKAAEETPAAPAELVRAFVDAGVPAGVIGLVYGNPAEISSYLIAH
                     PAIRKITFTGSTPVGKQLAALAGQHMKRVTMELGGHAPVIVCEDADLELAIKVSAASK
                     FRNAGQVCISPTRFLVHEAVRETFAAALARHASTLKVGDGLAEGTQMGPLANPRRVAA
                     MADFVQDALARGATVAAGGERIGAAGNFFAPTVLTGVPLDAKVFNEEPFGPVAAIRGF
                     TTLDEAIAEANRLSFGLAGYAFTRSLKNAHRLAHELEVGMLYVNQPATPSAEMPFGGI
                     KDSGYGTEGGPEALDAYLNTRAVTVMNV"
     gene            complement(29814..30965)
                     /locus_tag="PKN25_01000"
     CDS             complement(29814..30965)
                     /locus_tag="PKN25_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNV89501.1"
                     /translation="MNRRNFLRAGTLAAGLALPALARAQQSAATTATTPLATPAPLAE
                     PPAFAPTPDAGWRRFELTSRVEPLPGDGPLRVWVPLPAMHETAWQRPMGSLWQGNADL
                     MERVRDRSGAEMVYAEWAPGIAQPRLEILSRFATRDRAIDFSQPSAAPQVLPRAERMH
                     YLRATALLPTDGIVRDTARDIVHGAKTDEDKARAIYEWIVDNTFREPKVRGCGIGDIR
                     TMLETGNLAGKCADLNALFVGLARAAGLPARDVYGLRVADSRFGYKSLGKSGNVSKAQ
                     HCRAEVFLERFGWVPVDPADVRKVVLEEPPGKLSMVDPKVAAVRKQLFGAWEMNWLAY
                     NDAHDLRLPGSTGSEIPFLMYPQGELAGQRFDSLDPDAFSYTLSAREIA"
     gene            complement(30962..>31222)
                     /locus_tag="PKN25_01005"
     CDS             complement(30962..>31222)
                     /locus_tag="PKN25_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89502.1"
                     /translation="LDRDSRRSHDKARNTTVSAGATQVAISPAVHAASTIADNARRTR
                     VPILRIGLPRRGHTPHTAAGNGQPSSPGGIPHRGGFAHRENT"
     assembly_gap    31223..31322
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            31506..32222
                     /locus_tag="PKN25_01010"
     CDS             31506..32222
                     /locus_tag="PKN25_01010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV89503.1"
                     /translation="MRPFLHVGGRDYLRCEACEATFLHPAQRPAADAECAEYRLHRND
                     PEDPGYRRFLARLVDPLRIRLAPAAQGLDYGCGPGPALAAMLREAGHPMRVWDPFFAP
                     DPDALARRYDFVTCTEVVEHFHAPAAEFARLDGLLRPGGWLGITTCLQTEDARFAAWH
                     YRRDPTHVVFYRAATFRVIAARFGWRCELPAKDVVLLHKPAPPAAAAPCLSPPNPDRE
                     SPCATKPSSTTSPTASPRSA"
     gene            32165..32947
                     /locus_tag="PKN25_01015"
     CDS             32165..32947
                     /locus_tag="PKN25_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNV89504.1"
                     /translation="MRYETLLYDLSDGIAEIRLNRPQRLNAVTQRLYDELNDALGRAE
                     VAREVRVVLLTGAGRAFCVGADLKEHKAGRSAFDRRQYLQGEQDVCRRLIDLKKPVVA
                     AVNGYALGAGAEIALASDFLLMAEGAQLGLPEISIGNFLGGGVTWLLPRLVGLAKARE
                     LVFLGERIGGEEAVRIGLANRVFADAGFLDEARAFARRIAAKAPFSMELAKRQLNLSA
                     ERTLDACLVAELEGMMFVGTTRDWQEGVDAFAEKRAPVFRGE"
     gene            32949..35135
                     /locus_tag="PKN25_01020"
     CDS             32949..35135
                     /locus_tag="PKN25_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNV89505.1"
                     /translation="MNENSSPVPCSPARRSPLHDFLAPASIAIVGASADPTKRGYKAM
                     VGLVKGDYRGRVYPINPKVPEILGVPTCATLDDVPGPIDLALICTPAKTLPGLLEACG
                     RKGVKGAVILASGFRESGPEGARLEQQVLDAARAGGVRIIGPNTSGMFNLHHKVNLLD
                     LRNARPGDIGFISQSGNMLLALVLEAEHNGHVGFSTYVGPGNQTDVGFNDYLRYLGED
                     VHTRVATLYVEGFRDGQRFLEVAREITPVKPVVVYKSGATELGKKAASSHTGALAGSY
                     AMTVDLLRQVGVSVVHHSDEILPVAEGLALMQQARGRRIAVIADGGGQATITSDRLSE
                     SGLELAELSAATRKRLAAILFPQAALANPVDVAGSSDADPEVLARCVEIVADDDNVDA
                     VFLVGMFGGYHIRFAESLLGGEMRAAEAMIELARASAKPLVVYSLYAPVKPPALRRLH
                     EAGLPVYASIEQAVKVLEALAERGLYLQDRHGQPLQAGAAPDEAMQAMFARAQNEGRD
                     LYEHEARALLRTHGVAVAEEIVAHTADELAVAIERFRDQPLAMKVVSKDILHKSDAGG
                     VKLNLVGEAALREAWAQILAGCRAYDADADIRGVLLTPMARKGVEVIVGVVRDPIFGP
                     VLMFGLGGIFVEILEDVAFRAIPLSRRDAETMVDQLGARKVLAGVRGEAAVDKVALVE
                     LLLRVSSIVSAYPQLSELDLNPVIAYPDGYAVVDARIITNRTVASS"
     gene            35352..37220
                     /locus_tag="PKN25_01025"
     CDS             35352..37220
                     /locus_tag="PKN25_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV89506.1"
                     /translation="MENPEMARKRRSTIIWVIALAFVGLVFDGYDLVVYGAVLPGFMG
                     PDSAKWIGDHALTPKDAGMLGSYAMIGMMLGALVAGTLGDLIGRRKVMIGAIAWFSIG
                     MAVSAMTETLAAFGVWRFITGLGVGALAGTTGAMAAEFAPPGKKNLATAFTYAGIPLG
                     SLLSALLAIFLLPIIGWRGMFLIGALPLVTLLPLAIWKLPESVAWLASRGRLDEARKV
                     SERTGVPIPEAEIKPQQSAAANASDARAGWAGLFTVYLVPAIVIGFVSAFCLLLVYSL
                     NTWLPKIMLPIMGQNGSLALLLVLNAGAMVGTLYGSTLADKHSPQRIVALGFLVGALA
                     MGAIGMIASTIDVPMVAKAGEIQQLADVSGIVVALLLLTIAITGLGTSGTQTLIYGLA
                     ANYYRTNVRGAGVAWTAAFGRIGGIFGPIFGAFLAVTFAGELHSIFYVLGGISLVGLA
                     LTLIIPKSKAAAVAQAVAPTTAAAAAVPAGKSRLYGTIMAVVDHTGNRLTRERVSEFV
                     SLTGSKVHLVYLAPEHVLSAEVDQTDKAIDPAHVANLQEYVNEVNAQGVPAEGQILTS
                     TLFGRGRAVVDLAEQLRCDLIILNTEEGGQRAKAELAQEVAGQNPKMAVLIARSTQ"
     gene            complement(37394..37576)
                     /locus_tag="PKN25_01030"
     CDS             complement(37394..37576)
                     /locus_tag="PKN25_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89507.1"
                     /translation="MHIYFSHDGFLALALGLWELLRALRPKIAQTLTITARDPHPAPS
                     RSEESDVRAPQWRRHL"
     gene            37556..38911
                     /locus_tag="PKN25_01035"
     CDS             37556..38911
                     /locus_tag="PKN25_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="HNV89508.1"
                     /translation="MRKVDVHQTIDEAKFNKFHLNVLFWCALVIIFDGYDLVIYGVVL
                     PVLMKEWDLTPLQAGSLGSAALFGMMFGALIFGPLSDRIGRKKVIMITVIIFSGVTFL
                     NGFAETPTQFAAMRFIAGLGIGGVMPNVVALMTEYMPRKIRSTMVAVMFSGYSVGGMT
                     SAFLGMYLMPSHGWQSVFFVAGIPLLLLPLIWIFLPEAVGFLLKEGRKEEAGRLLARV
                     EPSYSPAAGDNFHLVTGQGRSVALVALFQNGRLVSTLMFWVAFFSCLLMVYALGSWLP
                     KLMNKAGYELGSSLSFLLVLNFGAIFGAIGGGWLADRFHLRRVLTIMFVIAALSISAL
                     AIKNDMIVLYLLVAIAGATTIGSQILLYAYVAQYYPLAIRSTGIGWASGVGRLGAISG
                     PMLGGALISLPLEMNFLAFAIPGAVAGLAISMVGRSAVHAPEEEKNLAFVGETGKLEV
                     D"
     gene            39062..40093
                     /locus_tag="PKN25_01040"
     CDS             39062..40093
                     /locus_tag="PKN25_01040"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNV89509.1"
                     /translation="MELEAYVRALPKAELHLHIEGTLEPEMMFALARRNGVALPWASV
                     EAVRAAYAFTDLQSFLDLYYAGAAVLVREQDFFELALAYFERAHADGVVHAELFFDPQ
                     THTARGVALETVLDGLERACVEARSRWGIGSRLILCFLRHLSEEEGFATLQQALPHLS
                     RIDGVGLDSSERGHPPAKFARLFARCRELGLHVVAHAGEEGPPAYIVDALDLLKAERI
                     DHGVRCTEDPALVGRLVREQVPLTVCPLSNVKLCVFPDLARHNLGQLFAAGLKVTINS
                     DDPAYFGGYVAKNYVDTARALGLGRAELRRIARNSLEASFVSAAERAPWLARLDALGE
                     DCEGEGGAA"
     gene            40090..41568
                     /locus_tag="PKN25_01045"
     CDS             40090..41568
                     /locus_tag="PKN25_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNV89510.1"
                     /translation="MTPSTAAERRALRDRLTAVPLFAGLPAVQLAELAAAARLLELPA
                     RTLVHGCGEPFDEAYVLCSGTVVRFRELDGDARKVVELVHTPQMLGCGEFFGASRHES
                     GCETASACALIALDAGVLRAVAERSLVLTRRMLQAVAARLCEVEFDVAGRHASRTSAQ
                     RILDYLVELAGGSLALAGETTVELASTKKVLAARIGITPEAFSRSLRELADKGVIVVD
                     KRLIHIQNAATLDTGAGEQPQRLSFSRRNKGARRDETMSVGELINLCGRLRLQVQRLA
                     IDWVLIGHGVAPVEMQLRLRQDIAEFERSLAVLQAASLAPELGEALEDVGRVWADFRD
                     ALGAAERPPAGAARVLQLSEDFITAADFLNAVAGGFAASGSLYMVNVAGRNRMLSQRV
                     AKFFLFEGFEGCPDPQAQIAASCRAFEHNLQRLRENAGELPELVAQIDATAALWARFI
                     AALDPAIRQPHRIGRVRAVLDEGERLLRYTNTLVKLFERLAH"
     gene            41715..41939
                     /locus_tag="PKN25_01050"
                     /pseudo
     CDS             41715..41939
                     /locus_tag="PKN25_01050"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
     gene            42117..42455
                     /locus_tag="PKN25_01055"
     CDS             42117..42455
                     /locus_tag="PKN25_01055"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNV89511.1"
                     /translation="MPADLNPSGDVFGGWIMSMVDIAGAVPAIRVARSKVATVAVDSF
                     VFKQPVSTGDIVSFHAEVVRVGRTSITVDVEVYAQRHPENPVVVKVTEARLTYVAVDE
                     FRAKRTIGAG"
     gene            complement(42464..42901)
                     /gene="trxC"
                     /locus_tag="PKN25_01060"
     CDS             complement(42464..42901)
                     /gene="trxC"
                     /locus_tag="PKN25_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HNV89512.1"
                     /translation="MSDALHLVCPHCDAVNRVPATRLAEAPNCGRCGKPLFTAHPVEL
                     DAANFDRHVGRSDVPVLVDFWAPWCGPCRTMAPAFAQAAGMLEPQLRLAKLDTEAHQA
                     LAARFGIRSIPTLALFRGGREIARQAGAMSAADIVRWVRGQLG"
     gene            43058..43645
                     /locus_tag="PKN25_01065"
     CDS             43058..43645
                     /locus_tag="PKN25_01065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNV89513.1"
                     /translation="MKLPRIVVMPGSRRREALSRRVAQACAQAVREAGAEVELVDLDD
                     YPAPLYDGDLEAASGLPEGIVRLQRVLHASDGLLLVNPEYNGSLTPLLKNTLDWCSRP
                     NPADAERSGGKVYAGRAAAVVGTSPGALGGMRVLFHVRDVLGYLGMQVIPQQVAVGQA
                     GEAVGADGRLLDAARQGMLEGLAEALVDTARRLRA"
     gene            complement(43651..44061)
                     /locus_tag="PKN25_01070"
     CDS             complement(43651..44061)
                     /locus_tag="PKN25_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNV89514.1"
                     /translation="MAGRKKNLPLVYSCSGCSSAAQMANHVALRLDREGEAEMSCIAG
                     VGGNVPHLVNIARSGRPILALDGCALECTRSSLAERGVRPAAHLLLNEQGVKKRYATD
                     FDREEAERVFEQARAAVRGLRATPATAMTGGTTD"
     gene            44242..44688
                     /locus_tag="PKN25_01075"
     CDS             44242..44688
                     /locus_tag="PKN25_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV89515.1"
                     /translation="MTKFPFLIAAAASVLLAACGNSGSGTPAAPAKAPAAPAAAAAPA
                     APAAAPAAAPVAAAPAAAPAGAAGEGTYKKVCALCHASGVAGAPIPGNKADWEPRVAQ
                     GKDTLYKHAIEGFTGSKGMMPPRGGGASLTDDEVKAAVDFMVGKLK"
     gene            complement(44776..45408)
                     /locus_tag="PKN25_01080"
     CDS             complement(44776..45408)
                     /locus_tag="PKN25_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNV89516.1"
                     /translation="MSIVRGRRRLLLAGAAVSLAAGSALLWDYTTRAGRSFEPPAEDI
                     CVVTPTRAAQAHPYDPASGLGKYDARPIPADARCPVCGMYPARFPRWAAQIVFEDGAA
                     HFFDSPVDLFMFLDKPASFGSTRTAAEAAAMYVADFRNGTWLPARHAVFVRDSRARGP
                     MRGPDLPAFADTAAAQAFIAEQGGRALTFEQVDASVVTSLRDANHAHHMH"
     gene            complement(45405..45779)
                     /locus_tag="PKN25_01085"
     CDS             complement(45405..45779)
                     /locus_tag="PKN25_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89517.1"
                     /translation="MSLHTELLDYVERAHEGALLAPARLSQDALNLVLRNGVVLTVRY
                     AAPDAYSLRWRTSADIDGVELGIDTAPVHPQLETTPNHLHRADGSVAADPLTDPRRSP
                     QENLARVIDVLGRDPQLADFGT"
     gene            complement(45779..46048)
                     /locus_tag="PKN25_01090"
     CDS             complement(45779..46048)
                     /locus_tag="PKN25_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89518.1"
                     /translation="MNPIFDAIPPEQALELEQIARLIYEARENRRKVLAAIGANDEAE
                     LLARIAAGAVDEHPGYEHYLAARILADTHEAARQVVGERLREINR"
     gene            complement(46066..46611)
                     /locus_tag="PKN25_01095"
     CDS             complement(46066..46611)
                     /locus_tag="PKN25_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNV89519.1"
                     /translation="MCTHHACPSRRRFLIAGVAAGLLAACGSGKQGAGSGTVTPLEID
                     RATSCALDGMLLADYPGSKVQMHYAGVERPDWFCDTIEMFNVYLNPEQARLVTAIFVQ
                     DMAKASWNAPQGHWIDAKKAWYVFGSRRMGAMGPTAASFANEADAKAFATENGGKVLR
                     FDEVTPDMAILDGGALHDQSM"
     gene            complement(46650..47465)
                     /locus_tag="PKN25_01100"
     CDS             complement(46650..47465)
                     /locus_tag="PKN25_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNV89520.1"
                     /translation="MEFSQIATIAGKEFWDRIRNRWVLAVALVFTVFALSIAYFGSAQ
                     QGAVGFRSIEVTIASLVSLVIYLIPLIALVLGFDAIVGERERGSLDLLLSMPITRLEL
                     LLGKYLGLAGALTFSTVAGFGLVGVVLSAQLDLGSLFHYFGFMLSSVLLGCAFLSIAV
                     MLSVFAADRTRASGLAIATWFFFVLIFDLLVLGVLVVTAGRWGGEALPYALLFNPADV
                     FRILNIFSMEDVRTLYGLSTVFPPALARPWLLGLVMAAWIAGPLAIATWRFRR"
     gene            complement(47476..48417)
                     /locus_tag="PKN25_01105"
     CDS             complement(47476..48417)
                     /locus_tag="PKN25_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV89521.1"
                     /translation="MADISPAQNDPGAPVIVARGVRKHYGPIHAVDGVDLEVRSGELF
                     GLIGHNGAGKSTMFKMMLGLIPLTAGEIRIDGAPVPGSDFRTVRRKVGYLPENVVLYD
                     NLTGTETLDFFARLKGVSTANNAAVLERVGLMHAAKRRVREYSKGMRQRLGFAQALLG
                     KPRILFLDEPTTGLDPEAIRGFYAILRQLKSEGVTMVITSHILAEIQERVDRLAIMAA
                     GKVQATGTVQALREQMDLPLWFDVRVAPEDFEAVRNALGGLPVGAIEARDGHVAVQCA
                     RDAKMAVIAALATLNGKVRDLTVREPSLEDVFFGFSD"
     gene            complement(48389..49675)
                     /locus_tag="PKN25_01110"
     CDS             complement(48389..49675)
                     /locus_tag="PKN25_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HNV89522.1"
                     /translation="MRSLHRLPRLLAAAALLAALATPAAATPWRVSPGESIQAAIDRA
                     APGDVIEIERGRYAGQLLVDKPLTLRGIERPTIDGELKENTISIKAEGVTIEGLIVAN
                     SGGDTLYQHAGVYIYPGSHKAVVRNCDFSYTLFGLWIEKANDVLIENNLITGKRDFDS
                     PKRGNGIQLYNTQRARIIGNNISFVRDAIYVDVSHDALFRGNKLHHSRYGTHYMTSYR
                     NVWEDNETWYNRGGLALMEVRDQVVRNNRAWGNSDHGIMLRSIQDAVIENNVVAGNQR
                     GFFIYDAEYNELRGNQIIDNVVGIHLWAGSKNNKVERNDFIGNREQIRYVAAHDMIWG
                     EKEGNHWSNYLGWDRNGDGLGDVRYEANDLVDRLTWRHPMMKLLLGSPAIQTLRMVAQ
                     QFPLLRAPSIVDPRPRMEPNKPDWSQWRGRHFPRSE"
     gene            complement(49828..50253)
                     /locus_tag="PKN25_01115"
     CDS             complement(49828..50253)
                     /locus_tag="PKN25_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription regulator"
                     /protein_id="HNV89523.1"
                     /translation="MVDPRILTEQVEPPYASRGSASRLPAEIWDHLWPWSRNGFQRQR
                     VVQAAGLALAAAASVAWILAAMGNMTPGAIIGWWFGWSVFEVAVRLGSKPYVKDGPWW
                     GSRYRRASIMDMICYVGFKNLLIGAALFIVLKSMGLVQV"
     gene            complement(50285..52468)
                     /locus_tag="PKN25_01120"
     CDS             complement(50285..52468)
                     /locus_tag="PKN25_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV89524.1"
                     /translation="MKPLSALRGIAMAALLAVTTLLPQSASAESAYEAKLPPEVNTAP
                     ALCDYVPCKEVLPGATSFSERKGQPPYVEGYAEENGQRKLVGYVVLSTDVTDTPAYSG
                     KPVITLIGLTPQGNFAGIKILKHSEPILLLGIPESALVKFNNQYLGKFVGANIEIGRS
                     RPEEEIIGLDAISGATVTVIAQNQVMMMSGAAVGRQVGILEKTVRPQARFVDSGRKLD
                     WATLVKEGVVQRLTVMPEQVGLERGKDPFIDLWYGYLNHPEVGRAIMGETGWRDLMAR
                     IKEGEHALFVIRTAGRESFKGSGFVRGGIYDRVQLRQGQDVFTFRDLDYYNLYGISAP
                     GAPSFTESAIFIVRSSSFSGAFPWKFIFLGNRIDRETGARTFINFDREYWLPDAYIEG
                     GRPEVKKPDAPWVRVWKTRALEIVLFSALLVAVAVVYAKRDELTRRSTRKNKWPVNAF
                     KYSAWIISIGFVGFGLLAQPSITQVLTWFHSLLFKWEWELFLTDPFIFLFWIFIIVTV
                     FVWGRGLFCGWLCPFGSLSELLYKVGGAIGLKRFQFALPMKWHNRLKWVKYAVFFGLL
                     AVSMFSMGLAEKLAEVEPFKTTFLVGLFNRSWPYTLFAAGLLGLSIFMERPFCKYLCP
                     LGASLAMPSTFRWFGLRRKQECDSCKACAVGCGSLAIDEKGRIDHRECMLCLDCMVLY
                     TDDHACPPLAQERKRRTKDGLPLTPIGKDGYYIPIKPVAPAAPRA"
     gene            complement(52652..>53212)
                     /locus_tag="PKN25_01125"
     CDS             complement(52652..>53212)
                     /locus_tag="PKN25_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNV89525.1"
                     /translation="KEASGQFLAVGCKFSKDRFLPVGPMHPENEQLIDIRGEKMVLLA
                     DHPVHPEPHDFIIVKRELIKTRQVGVLDAHPLAIKDAKESGVFRDGNKVTVRIASQAP
                     AYSLREFELKVGDEVTLILTNLDKVEDLSHGFAIPKYDINFVVNPLETKSVTFKADKP
                     GVFWCYCTHFCHALHLEMRTRMLVRP"
BASE COUNT         7688 a        17898 c        18955 g         8471 t
ORIGIN      
        1 aggcggccaa ggcgctggcc cgcttcatgc gcctgcaccc gcacaacatc ggccaaaaga
       61 ccgaggtgat ggtcgaacac ttccagcact tcactcggca caagatcggc ggccacgcga
      121 aggcgatggt ggtgaccggc tcgcggctgg aggcggtgcg ctacaagcag gaattcgatc
      181 gctacatcca ggaaaagggc tacccgatca agagcctggt cgcgttctcg ggcagcgttg
      241 aggacgacag gattgccgag aagacctata ccgaggtgga gatgaacggc ggcctcaagg
      301 aaaaggcctt gccggaaacc ttcgccaagc cggatttccg cgtgttgctg gtggccgaga
      361 aataccagac cggcttcgat cagccgctgc tgcacacgat gtatgtggac aagcgcctgg
      421 cgggcattca ggcggtgcag acgctgtcgc gcctgaaccg cacccatccc ctcaaggacg
      481 acaccttcgt gctcgacttc gtcaatgagc cgggcgagat ccaggaggcc ttccgccagt
      541 actacgaagg cgcggtgatg ggcgaggagg tcgatcccga caagctctac gcggtcaagg
      601 ccgagctcga tgcctcgggc atctaccgtc agaccgaagt gagcgatttt tcgcgggtgt
      661 tcttcgcgcc caagcgccgc cagagccccg gcgaccacaa ggcgatgaac gcgatcctcg
      721 accaggcggt cgcgcgcttc gtgcagttgc agaacacgga ggaggaggaa gccgaactgt
      781 ggcgcggcaa gctgcaggcc ttccgcaatc tgtacggctt tctgagccag gtgattccgt
      841 atcaggacag cgatctggaa aagctgttca cctacctgcg ccacctcgcg ctgaagctgc
      901 ccaagcgcaa gagcgggccc ggctaccagt tcgacgaaga ggtcgagctc gactactacc
      961 gcctgcagaa gatcagcgaa ggctcgatca gcctcgacga gggttatgcc aaaccgctgg
     1021 acggcccgcg cgaggtgggc tcgggcatgg tgcgcgagga gcatgtttcg ctctcccgcc
     1081 tgatcgacat catcaaccag cgcttcggcg gcgagctcaa cgaagcggac cagttgttct
     1141 tcgaccagat cgccgaggcg gcgagcttga acgagtcgtt gcagagggcg gccgaggtca
     1201 attccctcga caagttccag ctcgtgttca ggcaggtgct ggagtcgctg ttcatcgagc
     1261 gcatggagtt gaacgaggag ctgttcacag attacatggc caagccggag atgcaggcgc
     1321 tggtgtcgaa atggctggga ggccaggtct acgatcggct gtctgcggca agaaggcggg
     1381 tcagctgacg ccgcccggat tgcgcaacaa acccacgacg acatctcggg ccttcgcctc
     1441 accccgtcgc cgcacccacc gtccagtaaa acccgggcgc ggtgccgttg atcgcgtggt
     1501 cgcccactac ccgcgccttg aagatcaccg ggttgtgcga ggccagcgtg cgcgcgttgc
     1561 gccagtggcg gtccagccgc cggttttcct gcacggccga ggcgccgccg acgtcgaaca
     1621 gtcgcgtggt cgaggtcagc acggcgtcga ccaggccgac ctgggccttt gccgcggcaa
     1681 gttcgacgtc caccagcagg ctttcgggga cggcttcgcc acgccggcgc aggcggtcga
     1741 tgtcgccgag cctgtctgcc acggcgcgca cggcggcctc ggcgatgaag gcggtgctgg
     1801 cgagctggcc gacgacctgc tggatgagcg ggtcttcgcg cgggatggcg ccactgccgt
     1861 ggctgaacac gcgcttgcgc ggacgcacgt aggcgcaggc atcgcgctcg atggcacggg
     1921 cgatgccggt cagcgtggca aggtgcacga gctggaagaa ggcggtcatc gacgtcggcc
     1981 cgctgcgcgc gcaaggccag atcgaggcgg ggtcgacctc gacgtcggtg aggcgggtgg
     2041 tgcccgagcc ggtcaggcgc tggccaaagc ccttccagtc gtcgatgcgc tccacgccgg
     2101 gggcgtccgc ccgcaccaga gcgatgacgg gctgctcggc gccgcccgcc acgccctcct
     2161 cctgcagttg cgcgctcacc gcgatccagt cggcgtacag cgagccgctg ctgtagtact
     2221 tggtgccgtt caggcgcagc acgccttcct gcttcgtgat ccgcgtcgcc ggctggccga
     2281 gcgcgccgtc gccgatttcg gtggtggcgt tgccgaagat ctcgccggcg gcggcgcgct
     2341 ccagccaggg ggcatggagg gcggggtcga tctcggcgta gaggcgctcg atctggccga
     2401 aatgggcgcg gaagatgttg ggcaggttcg agtcggcttc ggcaagctcg atcagcagcg
     2461 cgaagaactg ctcgaccgag gcgccgaaac cgccgtgttc caccggtacg cgcagggcgc
     2521 cgaagcgggc ctcgcgcagc cagccgacgg cgtcgaaagg cagctcgcgg tcgtgctcgc
     2581 gctcggcggc gcgttcggcg atgcgggcga agatggggcg gaagcgcgcg gccagggtct
     2641 cgaacgccgg tgcgggcgcg gcggatgtcg gagaggccac cgcttcagcg ccgggcttcg
     2701 ttctgatgaa gggatggggc aggccggact gtcgtgcgtt catgcgaact ccacattgcg
     2761 ggtttcggtc ttgggggtga aggcgccgcg atagcgggcg gccggatggc gctcgttgat
     2821 gcggtcgctg ccgaacagct tctggcgcag ggtgccgggg cggtactcgc gcttggccag
     2881 gccgcgctgc tgcagcaccg gcagcaggtg ttcgttgaac tcctcgtagg agccgggcag
     2941 gtgccagttg atgacgttga tgccgtcgac gccggcctgc tgccacagct cgagctggtc
     3001 ggcgatctgc tccggcgtgc cggcgacacg gccgcggcgc gaggccaggc ggccgaggtc
     3061 ggccacggtc ggttcggtgc ccggcgagct cttcgcgtac cagtcgagga tgctctggct
     3121 ggcctcggac tggatcgccg ccagcggggt gtcggccggg tagggcttgc cgtagcggtc
     3181 gaccgcgtag ttgtggtggg cgaggaagcc gtcgacgctg gcgtactcgt cgtattccgc
     3241 ctccttgcgc cgcacttcgg cctcggtact gccgatgatg aagctcaggc cctggaagaa
     3301 cttgatgtct tcggcgcggc ggccggtggc caccgcgagg cggcgggtgt cgcggatcag
     3361 ctccgcagcc accgtgggat tgggggtgac gatgaactgg ccttcggcat tggccgcggc
     3421 aaactggcgt ccggcgcccg atgagccggc ctggaacagc agcggcgtgc gctgcggcga
     3481 gggcgcgctc aggtgcggcc cttccacccg gtaacgtggg ccgacgtggt ggatcttgtg
     3541 gatcttgttg tggtctgcga acacgccgcg cgcgcggtcg atcagcagcg cgccctcgtc
     3601 ccacgagcct tcccacagct tgtagacgac atcgacgtat tccgccgccc aggcgtagcg
     3661 ctcgtcgtgc ggggtgagct ggtcctggcc gaagttgcgc gcggcgttct ccaggtgggt
     3721 ggtgacgatg ttccacgcca cccgtccgcg cgagatgtga tccagcgtcg ataccttgcg
     3781 cgcgaagtcg aaggggtggg cctgcagcac cgaactggtg aaggcgagcc cgaggtgctc
     3841 ggtgttcacc gccagggccg acagcagcac cgacgggtcg ttgctcggga tctgcagccc
     3901 ctcccgggca ttgacctcgt agtcgccgcc gagcggacca tagaggccga ccacgtcggc
     3961 gaagaagatg gcgtcgaaca ggccgcgctc gagcgtgcgc gcgaggtcga cccacagatc
     4021 gacgtcgttg aaatcgacct gtcgggcatc ggggtgccgc cactgtccgt gctggatgtg
     4081 ggaggcggtg ttcatgacga aggcgttgaa ggcgagggcg ggcatggggt gtcctgttcc
     4141 gaggaatgga gggcggagat gcggcaacgg gcgggcgtcg ttcaagcgat ggcttcgctg
     4201 ctgcgtgtcg tggctgtgtg ggcggtccgg tctgggcctt gcggtgtggg cgtgatcggg
     4261 atgacggcgc tcgggttgcg gtctgcgcgc tggcgcgcga tcgcctccct ggcgacgcga
     4321 tcgatgacca tgtcgttgtg cggggggttg tagaggccgc attgcacgtc gcggaagtag
     4381 cgctccagcg gcaggtcgcg cgacagcgcg tggccgccgg cgatctgcat cgcgagttcg
     4441 acgatgcgga tggcgttgtt ggtcaccgcg tacttggtga tcgcgacctc gctgccgagc
     4501 ttgtccttga ggtcgtcgtg gcggtcccag cgctcggcca ggccgtacag cagcgatttc
     4561 gacacgccga tcaggatctc gatctcgccg atcttgtgct gcacctgcgg cgcgtccagg
     4621 atcacgccgc cgaggctggg cgcgaagcgg ctgtcggcga agtcgaggat gaagtcgcgc
     4681 gcggctaccg cgataccgag atagatcgcc ggcaactgca gcgagtaggc gccgctgcag
     4741 gccatgaagc ggctcggctt gcccttctcg aggcggacca gcagggcgtc cgcgggcacc
     4801 tccacggcgt cgagctcgac gtcgtgactg ccggtgctgc gcatgcccag cgaatcccag
     4861 gtttcgacga tgcgcacgcg cgcggacttg cgcaccagga actcggccgt ctggtcgtcg
     4921 tcctcgatat gggctagcac ggtgaagtgg ttcactgcgg gcgccagcgt ggagaaggcc
     4981 ttgcgccccg agatgaggta gccgtcgccg tggcggcggg cgacggtggt gggcttgaag
     5041 ccgcgcacga cgttgccggc atcgcgttcg gtggcgaaga cattgatcag ctcgccgcgc
     5101 tcgaccgcat cgcggcacac acgctcgaac acggcctcgt cccaggcgcg cgtggtgctg
     5161 aggccgaaga aggtcatcag gtgccagccg agcgcgagcg cggtcgcgcc gctggcgccg
     5221 gcgatgcgct cctgcaccag cagcgtctcg tacagcgaca gcccccggcc gccgtattcc
     5281 tcggggacgg tgaggccgag cgcgcccttg tgcctcagga tctcgaagtg ctcgaacggg
     5341 aattcgcccg ttcggtcgta gcgcgctgcc gtcggccgga ttgcggcggc gatgtcattg
     5401 gcgtaggtgg cgagttcggc ctcgcgcgcg ttgcgcaggg tcggatcttc gatgggcatg
     5461 ggtcttgctc cgcggctgct caggaataga aggaaggctt cgggtagcgg ccgttgagcg
     5521 cccagtcgcc gagttcgcgc accttgtaat ccaccgggtc gtgcagggta tgcacgcgca
     5581 ggttgcgcca gaagcggtcg aggcgcagcg cggcggtggt ggagcgcgcg ccggtgactt
     5641 cgaagatgcg gctggtgagg tcgagcccgc cgcgggtggc ggcgaccttg gcggtggcga
     5701 cggcgagcgc gacctcgccg cggccttccg tgctgagcgc gagccctgtg tcccaggcgg
     5761 cgtcgaaacg cgcagcggcg cggtcggcga gcgcgcgggt cgattcgagc gcgacccaga
     5821 actcgccgta gtgggcgagc acataggggt cttcgctgac gtgggcggcg ggcgaggccg
     5881 accacaggcg tgcctcgttg cgggtgtagt ggcgcgcttc ggcgagtgcg ccttcggcaa
     5941 tgccgaggaa gatgttcacg aagatgagct gcgcgagcag cgggcgcagg caggcgtggg
     6001 gcgaggacag cggcccgggc cgggacagga tctcgtcctc ggccaccgcg acgcgctcga
     6061 acagcgcact gccgctgtcg gtctggcgct gccccatgtt gtcccagtcc tccagcaggg
     6121 tgatgccggc gcggcgggtg gggagcgcgg cgatgatcag cttgccctcg gcgctgtcct
     6181 cgaccgccga ggccagcagc atgtcggagt cgagcgcgcc ggagcagaag ctcttgcgcc
     6241 ccgaaaactc gcgcccgcca tccacgcggg tggcgacggt gcgcttgtcg agcgggttga
     6301 gcgcgttgcc ccagaaccag ccctgcgtca ccgtgtcgag cagccagggc tgccactgct
     6361 ccgggctgcc gaacaggcgt accgaggcca gttgcaggtg gtggaaggcg aacacatgcg
     6421 ccaccgagct gtctgcccgt gccagctggc gtacgacgga cagcgtctcg gcccagctcg
     6481 cgccgaggcc gccgtatgcg cgtggaatgc tcagcttcag gaggccgctc gcgcgcagca
     6541 ggtcgcgctc ggccttcggc gtgcccccgg cgaggtcgcg cgcggcggcg gtttcggcga
     6601 agcgtgcggc caggcgtgcg gcgatggcgg cgggggattc cgcgcttacg gtattcggtt
     6661 cgagggtatg ggcgtcgctc agactcatgg gggatggttc ctttgccagc ggacaggtcg
     6721 gttcgagcgg ggaggggact ggaacggggg cggaaagcgg ggcggacatg ggtgacgaca
     6781 ttcaggcgtc ggccgcggcc agggcgtcgg cggcggtgtc gccgatgagg ccgaagcgct
     6841 tgagctgggt gcgcaggatg ttgcgcgaga tgccgagctg ctttgcggtg cggacctggt
     6901 tctcgcggca gtactcgaag gcggtcgtga atagcagttg ctcgatgcgc tcgtagacct
     6961 cgggcgtgtc ttcatccagc agccggcgca ggcccgcgcg cacggtggcg aagccgtctg
     7021 cgtggctgcc gccgggatcg ggcgtcgcac cgggaggcga cagcgcgaag ccgggcaggc
     7081 gcaggtcgcc gggattgacg acgccgttct ggcacacgat cagcgcgaag tggacgacgt
     7141 tctccagttc gcggatgttg cccggccagt ggtaggccac cagttccgcg gcagcttcct
     7201 ggctcaggcg cggtgtcggg atgtcgaggc gctggcggta gaagtcgatg aagtactcga
     7261 ccagcggcag gatgtcgccc gggcgctcgc gcagcggtgg cagctccacc ggcgcgacgt
     7321 tgagccggta gtagaggtcg aggcggaaat gcccggcctg caccgccttc tccagattga
     7381 cgttggtcgc cgccaccagg cgtacatcca ccgggatcgg cttgcgcgag cccagccgca
     7441 ccacctggcg ctcctgcagc acgcgcagca gcttgacctg catcgccagc ggcaggtcgc
     7501 cgacctcgtc gaggaacagc gtgccgccgt tggccgcctc gaaccagccg gcgcgcgcct
     7561 gctgggcgcc ggtgaaggcg ccggcctcgt ggccgaacag ctcggcctcg accaggttgt
     7621 cgctgaacgc gccgcaattg accgccacga aggggccgct gcgtccgctc tgcgcgtgga
     7681 tgtggcgcgc caccagctcc ttgccggtgc cggtctcgcc gatgatcagc acgttggctt
     7741 cggtcggcgc gatgagctcg acctggcgca gcaggcgcag cgatttggcg tcatggaaga
     7801 tcaacgcctt ggcgcggatg gagagcgcgc cttgcccggc gttgggcaag gtcagcagct
     7861 tggcggtgtt cgattcgggc atgttgcact cctgtgcggg ggcgaggccg cggaactcct
     7921 ggcagcggat caagagcggc gctcgtgttg cagtggggaa agcgcagcgg tcagggcgtg
     7981 ctcggcatgg tcgttctgcg caggctcagg aagcgtcgcg tctggtgccg cagccgggcc
     8041 agggcgccgc tgcggccgcg caacggcgct cgctggtccg gcgtgatcat cccgagcggg
     8101 aggtcgcccg cgctgtcgcg gagttgctgc aacaggctcg gagcgagcgg ccagggcccg
     8161 tgaccggact cgacattgat gtgtcctgca tcgcccgcat cgatcagcct gcttccccag
     8221 cgctcggccc attcatgcgc cacgtcctgg ctcagccacg ggtcgctacg gctgacgacc
     8281 atgaagctgg gacaggggag gccggattgc ggcaggaagc gtccgacccc ggcgctgcgc
     8341 tgtgggtcct cgcgcacgcc gaggagttcg aagcgctccg gatcggcggg cgccaccagc
     8401 agcaggcctg caaccttctg tggccgatcc accgcggcca ccacgctggc gaggcagccg
     8461 aagctgtgcg caacgatcca caccgcaccg gccgaggcgt cgatctcgcg ccgtacctcg
     8521 cctgcccagc gcgcgagtag cggggtgtcc cagtcgatgc cgccgacgcg ccgggcctcg
     8581 ggcagttcgc cctcgagcca gctctgccag tgcccggggc cgctgccgcg gtagccgggg
     8641 acgatcagca ccgtctggcg catgacggcc tcacagcgtg gcgatcgaca cttcggtgga
     8701 cttcaccagc gcgacgacct cggagccggg cttgagcccg agctcgtcga ccgagcgcgt
     8761 ggtgatcacc gaggtgacga tgcccgagga agtttccaca tcgacttcgc tgaccacctc
     8821 gccacggatg atttccttca cccggccgcg aaactggttg cggacgttga ttgcctggat
     8881 tgccatcgca gtgctcctcg cttacttggc gtagatctgg tcgaacaccc cgccgtcggc
     8941 aaagtgcttc ttctgcgccg cctgccagcc gccgaggtcc tcgatggtga tcagctcgac
     9001 tttcgggaag cgggcgacgt cggccgggtc ggcgtgttcg ggcctgaccg ggcggtagta
     9061 gtgcttggcg gcgagcttct gcccgaccgg cgagtacagg tactcgaggt aggcctgggc
     9121 gagcttctcg gtgccgcgct ttttcgccac gccatcgacg acggtgaccg gcggttcggc
     9181 gcggatcgag atcgagggca cgacgatctc gaacttgtcc gggccgagtt cgttgatcga
     9241 caggaaggcc tcgttctccc acgccagcag cacgtcgccg atgccgcgct ggacgaaggt
     9301 gttggtggcg ccgcgggcgc ccgaatccag caccggcacg tgcttgatca gttcggtgac
     9361 gaaggcctgc gcggtctgtt cgttgccgcc cggctgcttg agcgcgtagg cccaggcggc
     9421 gaggtagttc cagcgcgcgc cgccggaggt cttggggttg ggggtgacga cctcgacgcc
     9481 cggcttgacc aggtcgcccc agtccttgat gcccttgggg ttgcccttgc gtaccaggaa
     9541 gacgatggtc gaggtgtagg gcgagctgtt gttggccagg cgcttctgcc agtcggcggg
     9601 gatcttgccc gtctgctcgg cgatggcgtc gatgtcgtag gccagggcca gcgtgaccac
     9661 gtcggcttcg aggccgtcga tcaccgcgcg cgcctgcttc ccggcgccgc cgtgggactg
     9721 cttgatggtg acggtctcgc cggtgcgggc tttccagtgc ttggcgaact cggggttgaa
     9781 gtcctggtag agctcgcgtg tggggtcgta ggagacgttg agcagggtgg tctgcgcgtg
     9841 ggagacgccg gcgacgccgg tggcgagcgc cagggtgagc agggtgctgc gaagggggcc
     9901 gagcttcatt tctttctccg gatgggtgag tgcgatggcg aaaatttagc cttcgtgcgc
     9961 cttcacccga accaagaaaa aacggattgc ttattcgctc ggcggccggg aggcgacgac
    10021 cccgagagcg acgaccccgc gcacgacgag caccctgtgc gcgcggcccg tctttggaat
    10081 ccacgaccgg gttcgctaac atctcgcgtt ctttcgcccg ccccgcgacc gcggcgcggc
    10141 ctgtcacgcc ttcgcatggg gaattccaca aatgatcgtc ttcgtcaccg gcgcctccgc
    10201 cggcttcggc gccgcaatcg cgcgcagctt cgtcaagggc ggccaccgcg tcgtcgccac
    10261 cgcacgccgc aaggaccgcc tggatgcgct cgccgccgaa ctcggcgacg cgctgctggc
    10321 gctcgagctc gacgtgcgcg acgccgatgc ggtcgccgcg gtgccgggcg tgctgcccga
    10381 cggcttcgcc gaggtggacg tgctggtcaa caacgccggc ctcgcgctcg gcctcgaacc
    10441 tgcgcacaaa gcctcgctgg aagactggca caccatgatc gacaccaact gcaccggcct
    10501 ggtgcaggtc acccgcgcct tcctgcccgg catggtggcg cgcaaccgcg gccacatctt
    10561 caacctcggc tcggtggccg gacgctggcc ctatgctggc ggcaacgtct atggcgccac
    10621 caaggccttc gtgcgccagt tcagcctcaa cctgcgcgcc gacctgctcg gcaccgcgct
    10681 gcgcgtcacc gacatcgagc ccgggctgtg cggcggtacc gagttctcca acgtgcgctt
    10741 ccacggcgac gacgacaagg cggccaaggt gtatgccgac gtgcagccgc tgaccgccga
    10801 ggacatcgcc gactcggtgt actggatcgc cacccggccg gcgcacgtca atatcaacac
    10861 catcgagctg atgccggtgg cgcagtcctt cgccggcttg tcggtgcatc gcggctgagc
    10921 cctggccggc gtgcgaccac atccgcagcg gagcgggcag ggcgccgtcg ccgctccctc
    10981 gcgctggcgc cacgtcgggt tcgcttgcga cgatggccgc atgcgcctgc ccgggggggc
    11041 gaggcgggcg gcctgcggac gactccgtgc ctgtggcgag tttgtaacag tgtcattttc
    11101 ttgcagcagg atgcgcataa tttccgcaat gcatcatcga tccgcccgac cgtgactcca
    11161 gatcttccgc cttccgctcc ggagcccgca gtgccttccg tcgggcgccg cccgcgcccc
    11221 tggcgcgtgc tgggcgttgc catctgcgtg gcgctgctgg tggcgagcgt ggcgggcggc
    11281 ctgctgctgt ggcacgaggc cggtacctcg cgcctgcagg cgcgcgcgct cgcgcgttac
    11341 gccgcgcgcc tcgactacgc cctgctggcg gggccgagcg cggcgatccg ctttcctgcc
    11401 cacggcccct tcgaccagcg cctcggctac accgagctgg cgcgcttttc cgagcgcctg
    11461 caggcgcgcg gcttcgcact cgtcgagcag gtgcgcttcg acgatgcgct gctcgcccac
    11521 gtcgagcgcg gcctgttccc gccctacgcc gagaagacgc gcgcgggcct ggacgtcttc
    11581 gactgtcgcg gcgagccgct gtacggcttc cgctatccct ggcgcggata cgagcgcttc
    11641 gaggacgtgc cggcgatcgt cgcccaggcc ctgctcttca tcgagaaccg cgacctcctc
    11701 gacgaatcgc ggccgacgct caatcccgcg gtggactggg cacgcttcgc gcgcgcggcg
    11761 ctcggccagc tcggacgcat ggtcgatgcc gacctcgacg cgcccggcgg cagcacgctc
    11821 gccacccaga tcgagaagta ccgccactcg cctggcggca tcacccagga tgcgcgcgag
    11881 aagctgcgcc agatggtgtc cgcctcggtc cgtgcctatc gcgagggcga gcagacgctg
    11941 ccgctacgcc ggcggctggt gctcgactac ctgaacaccg tgccgctctc ggccgcgccc
    12001 ggtcacggcg aggtcaacgg actgggcgac gggctgtggg tgtggttcgg cgccgagttc
    12061 gcgcgcgtga atgccttgct ggcggcgccg gtgggcgagg gggaggagcg catcgcccag
    12121 ggtcgagcgc tgcgccaggt ggtcgcgctg atgatcgcgc accgtcggcc ctcgtggtat
    12181 ctcgccggcg ggcgcgagga gctggcgcgc accaccgacg cctacctgcg cctgttcgcc
    12241 gaggccgggc tgatcggcgc cgtgctgcgc gacgccgcgc tcgcccggcc gctcgccttc
    12301 cgcgacctcg tcgccgatcc ggcctgggtt cccgccacgc ccggaaaggg cacgaccgcg
    12361 gtgcgcaccc ggctcgccgg cctgctcgac accgcgctgt acagcctcga ccgcctcgac
    12421 gccgagctcg gcaccaccct gcacggcggc ctgcagcagg cggtgggcga ctacctcggg
    12481 cagctctccg acccggcttt cgcgcgtagc cagggcctga tcggcgagcg catgctgcag
    12541 ccggccaccc tcggcgcggt acgctacagc ttcacgctgg tcgagcgcac cgcgggcggc
    12601 aaccgcgtgc gcgtgcagac cgacaccacc gaccagccgc tcgacatcaa cgagggcagc
    12661 aagctcgagc tcggctccac cgccaagctg cgcgtgctgg cgacctatct cgagctggtc
    12721 gccgaactcc acggccggct cgcggcgctg gatcctccgg cgctgcgccg cgtcgaggtc
    12781 gacgcgcagg accgtctgac gcgctgggcg atcgaccacc tcgccagcgc gccggaccgc
    12841 agcctgccgg cgatgctcga ggccgcgctc gaacgccgct tctcggccag cccgggcgaa
    12901 ggcttcttca ccggcggagg ccgccacacc ttcggcaact tcaacgccag cgacaacgcg
    12961 cgcaatccca ccctgcgcga ggccctgcag gcctcgatca acctgccctt cgtgcgcctg
    13021 atgcgcgagc tcgtgcgtca caccatgtac caggtgccgg gcagcaccgc gcgcctgctc
    13081 gaggacaacg aggacccgcg ccgcgtcgag tatctggtcc gcttcgccga ccgcgagggc
    13141 cagctcttcc tgcgccgctt ctggcgcaag taccaggaca gcacggcgga ggagatccgc
    13201 tccaccctgc tcgacggcct gcgtccgggc gcggaccgcc tcgccgcggt gttccgctac
    13261 ctggagcccg aggccccgcc gcacgcgctc gccgatttcc tcgcccgccg cctcggcgag
    13321 ccccccgccg ccgcacgcct cgcccagctc tatgcgcgca acgccccgga agccttcgac
    13381 ctgcccgacc gtggctacgt ggcacgcgtg catccgctcg agctgtggct ggtcgccttc
    13441 cgcctgcagc accccgaggc cacgctggcc gaggcggtgg cggcgagcgc ggacgagcgc
    13501 cagcaggtgt atcgctggct gttccgcacc cgcgccaagg gcgcgcagga ctcgcgcatc
    13561 ttcaccctgc tcgaggtcga ggccttcctc gagatccacc gccgctgggc gcgcctgggc
    13621 tatcccttcg gccacctcgt gccttcgctc gccaccgcgc tcggcagctc gggcgatcgt
    13681 ccggcggcgc tcgccgagct gatgggcatc atcgtcaacg acggccagcg cctgcccgtg
    13741 cagcgcatcg acacggtgcg cttcgcgcgc gccacgccct acgagtccgc cttcgccatc
    13801 cgcccggtgg aaggcgaccc ggtgatggcg ccggaggtcg cgcgcgcgct gcgcggggcg
    13861 ctgtcggacg tggtcgaggg cggcaccgcg cgccgtctgg cgggcgcttt caagctggcc
    13921 gacggcagcg aactggcgct cggtggcaag accggcaccg gcgacaaccg catcgtggtg
    13981 cgcggacgcg ccggtcttgc gctcaaccgc accgccacct tcgtgttcta cctcggcccg
    14041 cgccacttcg gcacgctgac cgcctacgtg atcggccccg aggcggcgag ctaccgcttc
    14101 acctcgggct tgccggtaca gatcctgaag tcgatggggc ccttgctgat cccgcacctg
    14161 gagccggcgg gcgcgcatgc ctgcctgccg ccgcccttgt ccgaagccgc gccgcagccg
    14221 gtgacggccg gatagcgtgg atgtcggccc gcgggctcag ggctggaagg gccagaacag
    14281 cgggatcagc gcgctgcaca gcagccaggt gaggacgttc atgggcacgc cgaggcgggc
    14341 gaagtcggcg aagcggtagt tgcccgcgct atagaccagg gtgttggtct ggtagccgat
    14401 cggggtggcg aagctgcagc ttgcggcgaa catcacgccg accacgaagg ggcgcggatc
    14461 cacccccatc tgctgggcga gcccgatcac gaccggggtg aacagcaccg cgatcgcgtt
    14521 gttgctcagc acctcggtgg ccaccgaggt gatcaggatc accagcgaca acatcagcca
    14581 cggcgacatg ccctcgccga gcgcggcgag ctgggcggcg agcagctggt cgaggccgga
    14641 gttgcgcatc gcgatgctga tcgcgagcat gccgaagatc agcaccagga tcggccactc
    14701 gatcgacttg taggcctcgt cgctggtgat gcagccggtg gcgatcacga tcgccgcgcc
    14761 gatcagcgcc aggccctcga tcggcatcac gttcagcgcg gcgagcagca tcacgccgac
    14821 gatggtggcg agcgcgaccg gcgccttgtg gcggcggctg gccatctggc ggccctcggt
    14881 gatcgcgaac aggtcgccgt tgtcgcagaa gcgctggatc tgcgccggcg tgccctccac
    14941 cagcagcacg tcgccgccca gcagctggaa gtcctcgccg atctgcgcga acgaggcgtc
    15001 gcgccgatgc accgcgacga ggtggatgcc gtagcgcgcg agcaggtcga gatcgcggat
    15061 cgggcgcagg atgtagcgcg aggtctggcc gacgatcgcc tccaccatca ccaggtcgcg
    15121 ccggcgcagg gtctcgatgt cgtgggcctc ggcgcccgcc ggcgcctgca ggccgacgcc
    15181 gtcgctgctg cgcagcgcca tcatcgcgtc gctgcggctg tgcacgacca cgcggtcgcc
    15241 gacctccagc cgggtgtccg gggccgggtc ggcgagctcg atgtcgccgc gcaccaggtt
    15301 gagcacctgg atgatgccgt tcgacagacg cgcctcgcgc agcgtcctgc cggccaggtg
    15361 cgagccctcg ggcacgaaga gctcggtcat gaaccagcgc tcgccggcgc cgaactgctg
    15421 gctcagcgtc tcgcgcaccg gcagcaggcg cggcgcgaac accagcatga aggcgctgcc
    15481 caccagcgcc atgaggatcg ccggcaggct gatctcgaac atcgagaagg gcgccaggcc
    15541 ctgctcgcgc gcgacgccgt cgaccaggat gttggtcgag gtgccgacca tggtgatcag
    15601 cccgccgagg atggtggcgt aggagagcgg gatgagcagg cgcgaggggg cgaggccctg
    15661 gcgggccgcc accgcgatca ccgccgggat catcaccatc accaccgggg tgttgttgat
    15721 gaagggcgac accagcaggc aggcggcgat cagcgccagc agcaggcggc gctcgcccgc
    15781 gccggccgcg gcggcgagcc actcgccgag ctggtccacg cagccggtgc gcgacagcgc
    15841 cgcgctgatg atgaacaggc aggcgatggt gatcggggcg ctgttgccga acacaccgag
    15901 cacctccttg gtgttcagca tgcccgccac gagcagcacg cccacggcga ccatgaccgc
    15961 cacgtcgggc ttcacccact cgcgcacgaa ggcgacgaag aggacgacga ggacgagtcc
    16021 gacgaagagg ggggcgtgga gctcgggtag cggcatggcg gcacgagtgg gcgggggagt
    16081 ggagcttagc tgccgcgttt atgcattcaa aagacttgat attccatagc ttattcctct
    16141 cggttcattc gagcggtggc tgaagtcggg cggtgcgcgg cgatccacct cggcgccgca
    16201 gcccggcagc gggtgcctgc agtggcgcag ggggttcgat ctcgccgttg tcggcctata
    16261 tttgtcatgc gggtcccgcg aagccgccta cgaggggaaa gtcatgcgtc tccgtttctt
    16321 cctgtcgtcc ccgggcgatg tcgccgacga gcgcacattc gcccagcagg tcatcgagca
    16381 ggagctgccg aaggatccct tcatgcgcgg ccgggtcggt tgcgaggcgg tgcgctggga
    16441 cgaccctgcg gcaccggtgg cgatgccggc gacactgacg ccgcaggaag cggtcaatcg
    16501 cgggctgccg cggccttcgg cgtgcgactg cgtgatcgtc gtgttgtggt cgcggctggg
    16561 gacgccgctg ccggcgagct gcacacgccc cgacggcagc cgctatctct cgggcaccga
    16621 atgggagtac gaggatgcca tcgccgcgag gccgcagccc cacatcctcg tgtatcgccg
    16681 gcgcagcccg cccgagctcg gcgacccgct cgatgcggac tacgacgagc gcctcgccca
    16741 gtaccgcagg gcgaacgact tcttcaagcg cttccgcaac gcggatggtt cgcttgccgg
    16801 cggcttcgcc gagtacgaca cgccgcagga gttccgcgac cgcctgcgcg tggatctgcg
    16861 cgccttcatc cagcgccgac tcgacagcga gcaggccaac gggcagaccg tacgcgtcaa
    16921 cgcgccgccg ccgtatggac ggattgcgaa ggcgctcgcc agcggttctg cggtgccgat
    16981 catcggtccc ggcatctgcc gctcggggcg ccccggcgac gagccctggg accccgatgc
    17041 accgcgcttt ctccccagcg gcaacgagct gtcggcgtgc ctggccgacg aggcggggtt
    17101 cccgtcgcag gccgaacgcg accagctggc ggaggtggcg tcctactacg aggccttcaa
    17161 gacccgcgat gccctgcgcg agcgactgcg ttcgctgctc gcgccggccg cgccggacaa
    17221 gctcgccgtg ccgccgctct actcgttgct cgccagtctc gagaagccgc tgctgatcat
    17281 cacgaccaac tacgacaccc tgatcgagca ggtcttccgc aacgcgcaga aaccctacga
    17341 cctggtcgtg tactccgaac tcaaggagct cggcaacgcc gtcctgtggt ggccgcacgg
    17401 cgccctgaag ccggagacgg tggcgccgaa cgaactttcc atcgagctcg agaagaagac
    17461 ggtgatcttc aagatgcacg gctccatcgt tccccagacg tccgacctcg acaccttcgt
    17521 gatcaccgag tcggactatg tcgaactgct tgcgcgcatc gccagcaggt cggcgattcc
    17581 ggccctgttc gcgtcgcact gtcaggaccg caatctgctg ttcctcggct acagtctgcg
    17641 cgactggagc ttccgcacga tcctgcagag cctcaaccgc ttcttcgcca agcgcctcgt
    17701 cgccggcgac gacgagatcc agtcgtgggc gatcgacgat cgcttctccg agctcgagct
    17761 caagttctgg cagcggcgcg gggtgtatcc ctacgaggtg aacctggacg agttcgtccg
    17821 tttgctgcgc gcgaggatgt cgccatgaac gccgaggtcc gcatcgagca gcgttgcccc
    17881 tacgtcggcc tgcagccctt cgaggaggcc gaccgggcct tcttcttcgg gcgcgagcgc
    17941 gaccagcggg tgatcatcgc caacctgctc tcgtccccgc tgacgatcct ttatggatcg
    18001 agcggggtgg gcaagagctc ggtcctgatg gccggcgtcg tgccgcagct gcatcgcgag
    18061 cggccgcgga ccccggtggt ggtgttccgc aactgggtgg atcgcgactt ccagctcgcg
    18121 ctgacgcgcg cctgcatcga aaccgtctgg gccatgcagg tggaccagcc caggccggcg
    18181 gagaccctgc ccctcgacga ggtgctgcgc gcctgcgctg aagctgcgca tggcacgatc
    18241 ctggtgatct tcgaccagtt cgaggaatac ttcctctatc acgccaagga cagcgaggcg
    18301 gccggcgcgt tcgaggccca gttcgcgcgc gcggtcaacc gcgaggacgt ggatgtcggt
    18361 ttcctggtcg cgctgcgcga cgacagcctg tcgcggctgg accgcttcca ggagcgcatc
    18421 cacaacctgc tgagcaaccg cctgcggctc aaccatctgg agccggaggc ggccggcgac
    18481 gcgatccgca agccgttgga ggtgtggaac gcgctgcagg cggtgggcgt gccgccggtc
    18541 ggcatcgacg atgaactggt cgacgagctg ctccgccagg tccgcatcgg ccaggtctcg
    18601 gccggacgtc ccgggggcag cggggggctg cgccacgacg agggctgcat cgaggcgccc
    18661 ttcctgcagc tggtcatggt gcgactgtgg gaggaggagc gcgcgcaagg ctcggaccgg
    18721 ttgcggcggg agacgctcga ccggctgaag ggtgccgacg agatcgtccg cacccacctc
    18781 gacgtcgcga tggcccgcct cgacgcgacg agccaggcag tgtgcgccag cttcttcgat
    18841 cggctggtca ccccgagcgg cagcaagatc gcctgcggcg ccgcggacct gatcagctgg
    18901 gcgggggatc tcgcgcccca cgtcccggcg gtgctgcagt ccttgagcga cgcgcgcaac
    18961 cgcatcctgc gcaccgtgtc cgtccccgac aacccggagg tgatgcgcta cgagatctac
    19021 cacgacgtcc tcgcgcccgc catcctcgac tggcgccagc gcttcgaggc cgatcggcag
    19081 cgcgaggaag cgctccgggt ggctcgcgag aaggccgcac ggcgcgagga ggaggcgcag
    19141 atcgcgcgcg aacgcgcggc ccggcgcgcc ttgcacaggt ggctcgtggg cctgggcatg
    19201 atgagttgcg tcgccggtgc gggatggctc tatgcgtcct gggagggaat gcgttcggcg
    19261 gccaacgaga aggcggcgca gtcgatctcg aacgcgccta tcgatcccgg aagtgcgctg
    19321 agcctggcgc tggaggcggt ggaggcgacg gtcccgtcct ggatgtcggt cgctcgcgcg
    19381 gcccgcctgc acctgacccc gaccgctgcc gccgaagacg ccttgcgcca ggcgatccag
    19441 gcctcgcgcc tggtgtggac cctgccggtg agcggcaggg cgctctccga tgtggctttc
    19501 agtcccgatg gcggccggct gctcactgcc ggccaggatc agaccgcgcg ggtgtgggat
    19561 atccgcagcg gccgtcccgc cgagcccgtc gtcaccttcg cgcacgacgc ctgggtgcgg
    19621 cggggccgct tcctgcccga tggcgagcgc gtcatcacgg tgtccggggg cctggcctac
    19681 ctgtggtccc tcgcgcagcc gacggctccg ctgcgcagtt tcgagcatgg cgggaacatc
    19741 tattctgcct tcgataccag cgccgacggc cggcgcctcg cgaccgccgg ggatggcgcg
    19801 cagaaggcgc gcgtgatccg catctgggac gtcgacgcgg acgcgccgga gccgctgctc
    19861 accctggatg cggagggggc ctggatcatg gggctggcgt tcagcccgga tggctgctgc
    19921 gtcgccaccg ctgcggtcga gcgcggcaag gccgagcgaa gcgcgatcgc cctgtggggg
    19981 ctcgagcgcc ggcgcgagat cctctccctg ccgctcgagg agccgagtga cgcgatcgcg
    20041 ttcgcgcccg atggcagcgc gctggtcacc gccagccgtg atggctggct gcgggtgtgg
    20101 gagcccgcgg ggaaagggtt cgaggtgctg ctgcgcgaag cggcgggcgg cacgcaccgg
    20161 ggtgaggtgc tcgcgtggaa tgggcggacg ctggccggtc acggcgaccg catccgcaat
    20221 gtggaggtga gtgcggacgg ggccctgatc gccagcgcgg gcggcgacgg gacggcaagg
    20281 gtctggagcc ggcatacggg cgagaacctg cttacgctga cggggcacct ctcctacgtg
    20341 gaggcggccc gcttcagccc cgacgggcgt cacgtggcga cggcgagccg tgacggcacg
    20401 gtcaagttct ggaacatcga ggggcacgcc aacacggtca ccagcatcgc cttcagcccg
    20461 gatggtcgta ccctggccac cggcagcagc gaccgcaccg cccgcatctg ggacttcgcg
    20521 gacggtaccc cgcgactgcg ccataccctg cgcggtcatg ccgatcaggt gcatcgcgtg
    20581 gccttcgacc cttccggggc gcggctcgcg accgccgggc tggacaacca gctgcgcctc
    20641 tgggacgtcg cgagcggcga ggcggtcacg ctgccgagcg tgcctcccga gcatcggcac
    20701 aaggatcagc tcaggggggt ggccttcagt ccggacggcc gcctgctggc gagtgccggg
    20761 gccgacggcc atgcacggct ctatccgctt gcgggggagg gaagcccggt ctccgtgctg
    20821 catggcgggg cgacgatcca ggtgtcgggc gtggcctttc atcccggggg cgagcgctgg
    20881 gcgaccagcg gcttcgacgg caagctgaag ctgtgggatc tgtccgggac cgatctcggc
    20941 acggtcggct tgccggatga cgagcgcaag ctcggtatcc gcatcgccgg cctcgccttc
    21001 agtccggatg gacaatacgt cgctgcggtg gtgcagcgac gtgtgcgggt ctggccggtc
    21061 gcagccttca aggagccgga ggtccatccg cccgtgcgcc tgccgctcat cgagggcgcg
    21121 cgccactgcg cggcgctcgc ctacagcccg gacggccgct ggctggcggt gggctgcagc
    21181 gacgccggcg tgcgcctgtt cgagatgccc gcgggaacgc tcgtcaagac catcacggtg
    21241 cacaagaacg aggtccgcgg cgtcgccttc agcccggacg ggacgctgct ggccacggcc
    21301 agcaacgaca agaccttcca tgtctccccg ctcgccttcg aggcggtgta cgaggccgcg
    21361 cggcgcctgc agcaggcgac ggccgccgag cgctagtggt cggcgcgtgg cggcccaagg
    21421 ggcgcgcctt gcgccagcct agcgccgcgc cgcgtccagg cgcgcctgtg cgctgtgctc
    21481 gagctcggcg gcacgttcgc gcaggaactc gaggaaggcg ccggcgagcg gagtgaggcg
    21541 cttgccggcc ggatacacca cctgccactg ggtcgggagc ggggtgcctt ccacgcgcag
    21601 caccgcgatg ccttcgtcca ccgcatgtgc gagcgcgtgg gcggacagca gcgacagccc
    21661 cagcccgccc gccaccgcct gcttcaccgc ctcgttgctg ccgagttcca ggcgcgggcg
    21721 cagggcgagg ccgtgggcgg cgaagaactg ctcggccgcc tggcgggtgc cggagccggg
    21781 ctcgcgcagc acgaactcct cggcggcgat cgcctcgatc tccagctttc tgcggcatcc
    21841 cgcgagcgga tgcgcggtcg aggccaccac cacgagcgga ttcgacagga agggcgtggc
    21901 caccaccggc ggcgcctgcg gcggccggct catgatgtag aggtcgtcga ggttgtcggc
    21961 caggcgtcgg atcaccgcgt cgcggttgag caccgccagc gacacctcca ccgccgggcg
    22021 ctcgcggcgg aaatcgccga gcaggcgcgg gatgaaggtc tccgcggtgc tgaccaccgc
    22081 cagccgcagc cgccccacca catcgccgcg cagctcggcg agggtctcgt cgaggcgctc
    22141 cagctcctgc tcgacccggt tcgcggtgtc gagcaccacg cgcccggccg cggtcaggtg
    22201 caggcggcgg ccgacgcgct cgaacagtgg caggccgacc tgctcggcga gctggcgcag
    22261 ctgcgccgac agcgtgggct gggtcaggtg caggcgctgg gcggcgcgcg acacgctgcc
    22321 ctcgcgcgac accgcgagca gcaggcggag ctggtggaag gtcaggcgca tcggcgatgg
    22381 acgagcgtca ttcgagtgat agagaaaaat ctatggtaag ttgaagaata aatcattttt
    22441 ctcgatgtgt gcgccgcggc aaaattcgcc tcgcactgac gcagccgcgc ccccggagaa
    22501 ccggcgcggt gctcccccgg ttccgcgccc gcgcgctccg ttcatcgagg aaaacaacac
    22561 atggtcgatg ccgttccctt cttcttcgcc ctcggcgcgg tggcctcgct cgcccgctcc
    22621 ggcctgcgcc tgcccgccgc cgtcttcgag accctgtcga tctacctgct gctggcgatc
    22681 ggtctcaagg gcggggtgga gatcgcgcgc tcggagagcg ccacgctgtg ggtcgatgcg
    22741 ttgctggcga tcggcctcgg cgcgctgatc ccgctgctgg tcttcccgct cttccgcctg
    22801 cgcttctcgc gcccggacgc agcttcgctg tcggcgcact acggctcggt cagcatcgtg
    22861 accttcgcag tgggcgcggc ggtgctggcc ggacgcggca tcgaggtgga aggccacctg
    22921 gccctgctcg ccgccctgat ggaggcgccg gcgctgatcg tcgccacgct gatcgcgcgc
    22981 tggggggtga agtcggccga tggcagctcg accgccggcg cgctggtgca cgaggtcttc
    23041 gccaacaaga gcgtggtgct gctcggcggc ggcatcctga tcggctggat cgccgggccg
    23101 cagggcctgg agccgctgtc gccgctcttc gtcgatctgt tcaagggcgc gctgtgcctg
    23161 ttcctgctcg agatggggct gatcgccgcc gaccgcctgc ccgacctgaa gaaggcgggg
    23221 ctgttcctcg tcggctgcgc gctcgccctg ccgccgctgc tggcgctggc cggctggggc
    23281 gtggcgcagg cgatgggcct gggcgtgggc ggcacggtgc tgatgatgac gctggcgggc
    23341 agcgcctcct acatcgccgc gccgaccgcg atgcggatcg cggtgcccga ggccaacccg
    23401 gcgctggggg tggcggcggc gttggcggtg accttcccgt tcaacctgct gctcgggatc
    23461 ccgctgtacc tgtggtgggc gcaggcgatg gccggggcct gaggagggcg ccggggtggg
    23521 gaggcggcgg caaggcggcc tccgtcacgg acacggactc cgcttcgctg cgggaggccg
    23581 tacggccttc cgcctcgggg tccgcagctt cgaggtcgcc ggccgcggcg gccatcgcgc
    23641 gccgaggccg gcggcgttcg ggcggacgcg cccgcctcgt ccggcttgcc aatccgcgtg
    23701 cgacttgcta aaagtccgct cgggtgtctt cgacacccga tccggtccga cgcggggacg
    23761 accccgcccg ccctgcgcgc cttgcgctgc gaccgtcggt ttcccgcggg acgacccgca
    23821 ggacgaggcg tgggcccgac ccgcccgccg ctgcccgagt tcccttccgt gtccacaaaa
    23881 cccaagcttc gtagcttccc cgaccgcctg cgccagatcg cgatgttcga gatcggcggc
    23941 ctggtcctga tcaccccgcc cttcgcctgg gcgagcgggg tgccgatccg cgactccatc
    24001 ggcctgctcg cgctgctggc gctgatggcg gcgatctgga acggcgccta caacaccgcc
    24061 ttcgactgga tcgagggccg ccttaccggg cgcaccgccg accgtcgccc gttccggctg
    24121 cgtgccgtgc atgcgctcgg cttcgagtcc ggcctgctgg tgatgacgct gccggtgatc
    24181 gtgtggtgga ccggcatggg ctgggtcgag gccctgatcg ccgacatcgg cctggcgatc
    24241 acctacacga tctacgcctt cctgttcaac ctgggctacg accgcctgtt cccgatcgcg
    24301 cccgccggcg gcgaggcgga cgcggccgga ctggcggcgg agcgctgaga tggcgaccgg
    24361 cgacagcgcg ggtgatgtgg cggaggcggc ggtcgtcctc gacgccgacc tctccgcgct
    24421 caacaccttc ggcctgcgtg cgcacgcggc ccgcctgctg cgcgtgcgtt cggtcgcggc
    24481 gcttcaggcg gcgatcgccg cgccgggctg gagcgacacg gcgcgcctgg tgctcggtgg
    24541 cggcagcaac ctggtgctcg gcggcgattt cgccggcacg gtgctgcggg tggagattcc
    24601 gggccgccgc ctgttgcgcg ccgaggccga tgcctggatc gtcgagggcg gcggcggcga
    24661 gagctggcac gatttcgtgc gctggacgct ggcgcagggc tggcccgggc tcgagaacct
    24721 gtcgcttatt cccggcacgg tcggtgccgc gccgatccag aacatcggcg cctacggggt
    24781 ggaactggtc gagcgcttcg agtcgctcga cgcggtcgag ctcgacacgg gcgagctgcg
    24841 ccgcttcgat gccgcggcgt gcgccttcgg ctaccgcgac agcgtgttca agcgcactcc
    24901 cggaaggtgg ctggtcgtcg cggtgcgctt ccgcctgcca cggccgtggc gcgcgctcac
    24961 gcgctatgcc gatgtcgcgc gcgagctcga ggcacgcgcg ctcgcgcagc cctccgcgct
    25021 cgaggtctcg gatgcggtga tcgcgatccg ccgccgcaag ctgcccgacc cggcggtgct
    25081 cggcaacgcc ggcagcttct tcaagaatcc cgtcgtcgac gccgcgcgct gcgcacgctt
    25141 gctcgccgcg caccccgcgc tgccgcacta tccgcaggcc gacggcagcg agaagctcgc
    25201 cgccggctgg ctgatcgagc aggccggctg gaaggggcgc gacctcggcc cggtgggctg
    25261 ttacgagcgc caggccctgg tgctggtcaa ccgcggcggt gccaccggtg ccgacgtgcg
    25321 ccgcatcgcg caggcgatca tcgccgacgt cgaggcgcgc ttcggcgtgc gcctggagcc
    25381 cgagccggtc ttcatctgaa ccacgggcgc cgcaactccc gggcaacgcc ggcggcaacc
    25441 gcgtgatccg cgacggcatc gccgaggtgc tgcgcgagca ggcgtcgacg ctgaacttcg
    25501 acgtctaggt gccggtgggc cggctcggcg agtgcgtggc gcgcatgcgc gccaagctgg
    25561 agcgcggctg gctgacgtat tcgcgcaccc cggcggagat cgcgccgatg cgcacgctca
    25621 aggcggcact cgacccgcgc cgcatcctca acccgggcaa gatgctgtag ggccgcgggc
    25681 cgccgcggcc ctagatgatg cggcgcggct gctcgatgat ggtctgatag accaggctct
    25741 tgatcttcat gcggtcttcc gcgccgagtt cgtggaagtc cactccgtag tggtactcga
    25801 tgccttcggc ctcggcgtcg gctgcctgct gcacgttgcg gatctcggcc tcgatgtcga
    25861 gctcggtctg caggccgtga aggtccacgt cgaagcgcag acggacgatc gcgcccggtt
    25921 cgccgaggga ggcatgggtt tggatcaacg cgccggtcgc gctcaggttc agcatcgtgc
    25981 cctcgcccgc cgcgccgccg gcctgggtgt tcacctgtac cggaaactgc gtgcgcaccc
    26041 gcgtcgactt gcggatcacg ctgccctgga tcacgctcgg aaagctcagg tgtacatagt
    26101 cgagaggcaa cctgcatgcg cgcagcaccg aggcgcggaa ggcgaaggca ctctggcgcg
    26161 agaacacccg cacgatcacc ggatcgttct cgaccagttc cacgcgccgc ccgcccgcca
    26221 ccggcgtggt caccagcagg ctgatcccgt ccacatagcc gaccacacgc acgaatgcgc
    26281 gccgcacgcc ggtgctcgcg gcgcattcga tctgcaggcg gtcgccgacc gccagccgca
    26341 tgtcctcgaa ggtgtagccg cgttgcgccc cgtcctcttc gtgttcctcc gcaagctcgt
    26401 cgcgctgcaa atcctcgttg cccgtgtctt cgctcatcgt cccgcccggc cgttccggga
    26461 ttgccccgat ggccaattga agctgctccg cccgtcgccc gcaagggcgg attgcacgcc
    26521 cggcctcgcg aatatccgct tttcctgatg tgtcgtatca cgatgggtgg atccggtccc
    26581 ggtgcggggc ctgctagttt ggcggccgtg cgcacgacgc tgcgccgcag aagccaatca
    26641 gtggacagga acggagacac aaaatgacaa cgacccgccg catcgcccca ctcgccctcg
    26701 cctgcgccct cctcgccgcc cccgcggcga gccaggccgc gacctggagc gacaccttcg
    26761 tcggctaccg ttacggcagc gacttccgcg agcccaccaa cacgaaggac gtcgagaagc
    26821 acgtgctgca gttcacccac gccagtggct attcggtcgg gcagaacttc ctcaacctgg
    26881 acatcttcca gtcggacaag gccgatccgg ccaacggcgg cggcagcggg gcgaccgagt
    26941 tctacctcac ttaccgccac caggtgcatc tcggcaaggc gttcgaccgc agcttcgcct
    27001 tcggcccggt caaggaggtc gcggtcaccg caggcttcga tctcaacacc aagaacaccg
    27061 cgttctcgcc gcgcaagcgc ctcgtggtgc tggggcccac gctgaagttc gatgtgccgg
    27121 gtttcctcga cgtcagcctg ctcctgggca aggagtggaa ccgctgcggt ctcgaccccg
    27181 tcgcgccgtc gtccttcgat ccctgcccgc agaccgaggt gagcttcgac ccgcaatgga
    27241 tcgtgagcgc ggcctggggc attcccttca gcgccggcgc cgtgccgctg aagttccagg
    27301 gcttcatcaa catcaacggc gagaagggca aggactacgc cggcatcaag acccacaccg
    27361 agaccctgat gcgcacctcg ctgatggtcg acgtcggcca gatggcctgg ggcaagaaga
    27421 acaccttcct gatgggcgtc ggctacgaat actggcgcaa caagttcggc aaccacgctt
    27481 tcgcgaacgg cgcagaaaag cccggcatcg acaccgacgc gccgaccttc cagatggagt
    27541 ggcatttctg agcgcatcga tcgcgactcg cgtcgctcct acaaaaccgc cgcgcctccc
    27601 gcgccgcgct tccggtgccg cgcggtggga gcgacgcgag tcgcgatcga cggtcgggca
    27661 gggtgccgcg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngccgcgcgg
    27781 tgggagcgac gcgagtcgcg atcgacggtc gggcagggtg ccgcgtgaca gccaccgcgg
    27841 cgcgcaaggc gccgcacgca acccgaatcc gaggtggcgg gctgccgcga tgcggcgagc
    27901 cctgcgccgt caccgcatcg cggcagcgac ttacacgttc atcaccgtca ccgcgcgcgt
    27961 gttcaggtag gcgtcgagcg cctccggccc gccctcggtg ccgtagcccg aatccttgat
    28021 gccgccgaag ggcatctccg cgctcggcgt cgcgggttgg ttcacgtaca gcatgccgac
    28081 ctcgagttcg tgcgccaggc ggtgcgcgtt cttcagcgag cgcgtgaacg cgtagcccgc
    28141 cagcccgaag gacaggcggt tcgcctccgc gatcgcctcg tccagcgtgg tgaagccgcg
    28201 gatcgccgcg accggcccga agggttcctc attgaacacc ttcgcgtcca gcggcacgcc
    28261 ggtcagcacc gtgggcgcga agaagttgcc cgcggcgccg atgcgctctc cgcccgccgc
    28321 cacggtcgcg ccgcgcgcca gcgcgtcctg cacgaagtcc gccatcgccg ccacgcggcg
    28381 gggattggcc agcggaccca tctgcgtgcc ctcggccagg ccgtcgccga ccttcagcgt
    28441 gctcgcatgc ctggccagcg cggcggcgaa ggtctcgcgc accgcctcgt gcacgaggaa
    28501 acgcgtgggc gagatgcata cctggccggc gttgcggaac ttcgacgccg ccgacacctt
    28561 gatcgccagc tcgaggtcgg cgtcctcgca cacgatcacc ggcgcatggc cgccgagctc
    28621 catcgtcacc cgcttcatgt gctggccggc gagcgccgcg agctgcttgc ccaccggagt
    28681 cgagccggtg aaggtgatct tgcggatcgc cgggtgggcg atcaggtagc tggagatctc
    28741 cgccgggttg ccatacacca gcccgatcac gcccgccggc acgccggcat cgacgaaggc
    28801 gcgcaccagc tcggccggcg ccgcgggcgt ctcctcggcg gccttgacca ggatggagca
    28861 gccggtggcg agcgccgccg cggccttgcg cacgacctgg ttgatgggga agttccacgg
    28921 cgtgaaggcg gcgaccgggc cgaccgggtc cttgatcacc atctgcgtgg cggcgaggtt
    28981 gcgtcccggc acgatgcggc catacacgcg caggccttcg tcggcaaacc actcgatgat
    29041 gtcggcggcg gcgagtgttt ccattttcgc ctcgggcagc ggcttgcctt gttcctgggt
    29101 gagcacgcgg gcgatgtcgc cggcgcgctc gcgcatcagc gctgcggcgc ggcgcagggt
    29161 cttggcgcgc tcgatcgccg gggtgcggcg ccaggtctgg aagcccttca cggcggcggc
    29221 gacggcgagg tcgaggtcgg cgatcgcggc atgcgcgacg cggccgatct cctcgccggt
    29281 ggccgggttg tgcacggcca gcgtgcggcc gtcgagggcg tcgcaccatt ggccgtcgat
    29341 gagcaggcgg gtatgggggt aggtcatggg ggcttcctct cgtgggaatg tgggacaggg
    29401 cattctagcg ggtggcggat gacggaagcg cgcatgggcg cgccgggtcg ggccccgtcc
    29461 ggggccgcgg tgtcgggcgc cgcgcggggc gtacactcgc gacccttcgc tggcgctcag
    29521 ggcggccctc ggtggaggac gccgcggggg cggcgacgca aacgcgtcgc tgcgtgcctc
    29581 ggacctgcgt cgccttgttt cccccgcgac gtcctccacc tcggcgctcc tgatcttccc
    29641 gcgcggcacc cgatgccgcg gccgggaccg tttgcggtgg cttgcaggca cggggggccg
    29701 cgtggcctgc agcgctcgcg caggtgcggg ggcttcgatg ccgcaggctg cggcgcccgg
    29761 ccgagggctc gggatcgcgc gaggtttcgc gctcgcacgc ctgtgttccc gcctcaggca
    29821 atttcccgtg cgctcagcgt gtagctgaag gcgtcggggt ccaggctgtc gaagcgctgg
    29881 ccggcgagtt cgccctgggg gtacatcagg aaggggatct cgctgccggt gctgccgggc
    29941 aggcgcaggt cgtgggcgtc gttgtaggcc agccagttca tctcccaggc gccgaagagc
    30001 tgcttgcgca ccgcggcgac cttgggatcg accatggaga gcttgcccgg cggttcctcc
    30061 aggacgacct tgcgcacgtc cgcggggtcc accggcaccc agccgaagcg ctccaggaag
    30121 acctcggcgc ggcagtgctg ggccttggag acgttgccgc tcttgcccag gctcttgtag
    30181 ccgaagcgcg agtcggcgac gcgcaggccg tagacgtcgc gtgccggcag cccggctgcg
    30241 cgcgccaggc cgacgaagag ggcgttgagg tcggcgcact tgcccgcgag gttgccggtc
    30301 tccagcatcg tgcggatgtc gccgatgccg cagccgcgca ccttgggctc gcggaaggtg
    30361 ttgtccacga tccattcgta gatggcgcgc gccttgtcct cgtcggtctt cgcgccgtgg
    30421 acgatgtcgc gtgcggtatc gcgcacgatg ccgtcggtgg gcagcagcgc ggtggcgcgc
    30481 aggtagtgca tgcgttcggc gcgcggcagt acctggggcg cggcgctggg ctggctgaag
    30541 tcgatggcgc ggtcgcgggt ggcgaagcgg ctgaggatct cgagccgggg ctgggcgatg
    30601 cccggcgccc actcggcgta gaccatctcg gcgccggagc ggtcgcgcac acgttccatg
    30661 aggtcggcgt tgccctgcca caggctgccc atcgggcgct gccaggcggt ctcgtgcatc
    30721 gccggcagcg gcacccacac gcgcagcgga ccgtcgccgg gcaggggttc gacgcggctc
    30781 gtcaactcga agcggcgcca gccggcgtcc ggcgtgggcg cgaaggcggg gggctcggcg
    30841 agcggggcgg gggtggcgag cggcgtggtc gccgtggtcg cggcggattg ttgggcgcgc
    30901 gcgagcgcgg gcagcgcgag gccggccgcg agcgtgccgg cgcgcaggaa gttgcggcgg
    30961 ttcatgtgtt ctcccgatga gcgaagccgc cccggtgcgg gatgccgccg gggcttgatg
    31021 gttggccgtt gccggcggcc gtgtgcgggg tgtgtccgcg gcgcggaagg ccgatgcgaa
    31081 ggatcggcac gcgggtccgg cgcgcattgt cggcgattgt ggaggccgcg tgaaccgccg
    31141 gggagatcgc gacttgcgtc gctcctgcag agaccgtcgt gttgcgagcc ttgtcgtggg
    31201 agcgacgcga gtcgcgatcc agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31321 nnccagtggc tccgatgtgc cgcgcgcgga tccgcacgag cgtcagatgc agccccgtgc
    31381 tccgcctgcc cccgggttcg ggatctccgc catgggcagg gccggcgggg ctcgatacaa
    31441 tcgcccgcat gaatcagccg gctcccggga cggcgacttg ctgcccggtc tgtcttggcg
    31501 catccgtgcg cccctttctg cacgtgggcg ggcgcgacta cctgcgctgc gaagcctgcg
    31561 aggcgacctt cctgcacccg gcccagcggc cggcggcgga cgcggagtgc gccgaatacc
    31621 gcctgcaccg caacgacccc gaggatcccg gctaccgccg cttcctcgcg cgcctggtcg
    31681 atccgctgcg catccgcctc gcgcccgcgg cgcaggggct ggactacggc tgcggtccgg
    31741 ggccggcgct ggccgcgatg ctgcgcgagg ccggccaccc gatgcgggtg tgggacccct
    31801 tcttcgcccc tgaccccgac gcgctcgcgc gccgctacga cttcgtcacc tgcaccgagg
    31861 tggtcgagca cttccacgcg cccgcggccg agttcgcccg cctggacggt ctgctgcgcc
    31921 ccgggggctg gctcggcatc accacctgcc tgcagaccga ggatgcgcgc ttcgccgcct
    31981 ggcactaccg gcgcgatccc acccacgtgg tgttctatcg cgcggccacc ttccgggtga
    32041 ttgccgcccg cttcggctgg cgctgcgagc tgccggccaa ggacgtggtg ctgctgcaca
    32101 agcccgcgcc gccggcggcc gccgcgccgt gcctttctcc ccccaacccc gaccgggagt
    32161 ctccatgcgc tacgaaaccc tcctctacga cctctccgac ggcatcgccg agatccgcct
    32221 gaaccgcccg cagcgcctca acgcggtgac gcagcggctc tacgacgagt tgaacgacgc
    32281 gctcggccgc gccgaggttg cgcgcgaggt gcgcgtggtg ctgctcaccg gcgcgggccg
    32341 cgccttctgc gtcggagccg atctcaagga acacaaggcc ggacgcagcg ccttcgaccg
    32401 ccgccaatac ctccagggcg agcaggacgt gtgccgccgc ctgatcgacc tgaagaagcc
    32461 ggtggtcgcc gcggtcaacg gctacgcact cggcgccggt gccgagatcg cgctcgcgtc
    32521 cgacttcctg ctgatggccg agggcgcgca gctcggcctg cccgagatct cgatcggcaa
    32581 cttcctcggc ggcggcgtga cctggctgct gccacgcctg gtcgggctgg cgaaggcgcg
    32641 cgagctggtc ttcctcggcg agcgcatcgg tggcgaggag gcggtgcgca tcgggctggc
    32701 gaaccgggtg ttcgccgacg cgggcttcct cgacgaggcg cgcgccttcg cgcgcaggat
    32761 cgccgccaag gcgccgttct cgatggagct cgccaagcgc cagctcaatc tttcggccga
    32821 gcgcacgctc gacgcctgcc tcgtcgccga gctcgagggc atgatgtttg tcggcaccac
    32881 gcgcgactgg caggagggcg tggatgcctt cgccgagaag cgcgcgccgg tgttcagggg
    32941 ggaatgacat gaacgagaac tcgagccctg tgccctgcag tccggcgcgc cgcagcccgc
    33001 tgcacgattt cctcgccccc gcctcgatcg cgatcgtcgg cgcctcggcc gatcccacca
    33061 agcgcggcta caaggccatg gtcggcctcg tcaagggcga ctaccgcggc cgcgtctacc
    33121 cgatcaaccc caaggtgccc gagatcctcg gcgtgccgac ctgcgccacg ctcgacgacg
    33181 tgcccgggcc gatcgacctg gcgctgatct gcaccccggc gaagaccctg cccgggctgc
    33241 tcgaggcgtg cgggcgcaag ggcgtgaagg gcgcggtgat cctcgccagc ggctttcgcg
    33301 agagcgggcc ggagggcgcc aggctcgagc agcaggtgct ggacgcggcg cgtgcgggcg
    33361 gcgtgcgcat catcggcccg aacacctcgg gcatgttcaa cctgcatcac aaggtgaacc
    33421 tgctcgacct gcgcaacgcc aggcccggcg acatcggctt catctcgcag tcgggcaaca
    33481 tgctgctggc gctggtgctg gaggccgagc acaacggcca cgtgggtttc tcgacctacg
    33541 tcggcccggg taaccagacc gacgtcggct tcaacgacta cctgcgctac ctcggcgagg
    33601 acgtgcacac ccgggtggcg acgctgtatg tggagggctt ccgcgacggc cagcgctttc
    33661 tcgaggtggc gcgcgagatc accccggtga agccggtggt ggtgtacaag tcgggcgcca
    33721 ccgagctcgg caagaaggcg gcgagctcgc acaccggcgc gctcgccggc agctacgcga
    33781 tgacggtgga cctgctgcgc caggtggggg tgagcgtggt gcatcactcc gacgagatcc
    33841 tgccggtggc cgagggcctg gcgctgatgc agcaggcgcg tgggcggcgg attgcggtga
    33901 tcgccgacgg tggcggccag gcgacgatca cctccgaccg cctgtcggag tccggcctgg
    33961 aactggccga gctgtccgcg gcgacccgca agcgcctggc ggcgatcctg ttcccgcagg
    34021 ccgcgttggc gaatccggtg gacgtggccg gcagctcgga cgccgacccc gaggtgctgg
    34081 cgcgctgcgt ggagatcgtc gccgacgacg acaacgtgga cgcggtcttc ctcgtcggca
    34141 tgttcggcgg ctaccacatc cgcttcgccg agtccctgct cggcggcgag atgcgcgcgg
    34201 ccgaggccat gatcgagctc gcgcgcgcca gcgcaaagcc gctggtggtc tacagcctct
    34261 acgccccggt caagccgcct gcgctacgcc gcctgcacga ggccgggctg ccggtgtacg
    34321 cctccatcga gcaggcggtg aaggtgctgg aggcgctcgc cgagcgcggg ctctacctgc
    34381 aggaccgcca cggccagccc ctgcaggccg gcgcggcgcc cgacgaggcg atgcaggcga
    34441 tgttcgcgcg cgcccagaac gagggccgcg acctctacga gcacgaggcc agggcgctgc
    34501 tgcgcacgca cggtgtcgcg gtggcggagg aaatcgtcgc ccacacggcg gacgagcttg
    34561 cggtggcgat cgagcgcttc cgcgaccagc cgctggcgat gaaggtggtg tcgaaggaca
    34621 tcctgcacaa gtccgacgcc ggcggggtga agctgaacct ggtcggcgag gcggcgctgc
    34681 gcgaggcctg ggcacagatt ctggcgggct gtcgggcata cgatgccgac gccgacattc
    34741 gcggtgtgct gctgacaccg atggcgagga agggggtgga ggtcatcgtc ggcgtggtgc
    34801 gcgacccgat tttcggcccg gtgctgatgt tcggcctggg cggcatcttc gtcgagatcc
    34861 tcgaggacgt cgccttccgc gcgattcccc tgtcccggcg cgacgccgag accatggtcg
    34921 atcagctcgg cgcgcgcaag gtcctcgccg gcgtgcgtgg cgaggcggcg gtggataagg
    34981 tcgcgctggt cgagctgctg ctgcgggtgt cgagcatcgt cagtgcctac ccgcagctct
    35041 ccgagctcga cctcaacccg gtgatcgcct acccggacgg ctatgcggtg gtcgacgcgc
    35101 gcatcatcac caaccgcacg gtggccagca gctgagccgg tcgagcggat tttcgcgcct
    35161 tggatcgggg tgtgcacgaa gcggatatcg atcgtgcaca agctggatat tgatcggtgt
    35221 gatcgcgcca taacctgacg gccttgcaat caaggaagct tgacgcacgt caagttcgtc
    35281 actgctcgcc cgtccgatct cccctgcagc aattccccca aagaggcacg attttgaaaa
    35341 cgaacgttcc gatggaaaac ccggagatgg cgcgcaagcg gcgcagcacc atcatctggg
    35401 tgatcgccct ggccttcgtc ggcctggtct tcgatggcta cgacctcgtc gtctatggcg
    35461 cagtcctgcc cggcttcatg gggccggact ccgccaagtg gatcggcgac cacgcactca
    35521 cgccgaaaga cgccggcatg ctcggctcgt acgcgatgat cggcatgatg ctcggcgccc
    35581 tcgtcgccgg caccctgggc gatctgatcg gccgccgcaa ggtcatgatc ggcgccatcg
    35641 cctggttctc gatcggcatg gcggtgtcgg cgatgaccga gacgctcgcc gccttcggcg
    35701 tctggcgctt catcaccggc ctgggcgtgg gcgcgctggc gggcaccacg ggcgcgatgg
    35761 cggccgagtt cgccccgccg gggaagaaga acctcgccac cgccttcacc tatgccggca
    35821 tcccgctggg ttcgctgctg tctgcgctgc tggcgatctt cctgctgccg atcatcggct
    35881 ggcgcggcat gttcctgatc ggcgcgctgc ccctggtcac cctgctgccg ctggcgatct
    35941 ggaagcttcc cgagtcggtg gcctggctgg cctcgcgcgg ccgcctggac gaggcccgca
    36001 aggtctccga gcgcaccggc gtgcccatcc ccgaggcgga gatcaagccg cagcagagcg
    36061 cggccgccaa tgcctcggac gcgcgtgcgg gctgggcggg tctgttcacc gtctatctcg
    36121 tcccggccat cgtcatcggc ttcgtctcgg cgttctgcct gctgctggtc tattcgctga
    36181 acacctggct gccgaagatc atgctgccga tcatgggcca gaacggatcg ctcgcattgc
    36241 tgctggtcct caacgccggc gccatggtcg gtaccctgta cggctccacc ctggccgaca
    36301 agcacagccc gcagcgcatc gtggcgctgg gcttcctggt cggcgcgctc gccatgggcg
    36361 ccatcggcat gatcgcctcc acgatcgacg tcccgatggt cgccaaagca ggcgagatcc
    36421 agcaactcgc cgacgtcagc ggcatcgtcg tggccctgct gctgcttacg atcgcgatca
    36481 ccggcctggg gacctcgggg acgcagacgc tgatctacgg tctggccgcc aactactacc
    36541 gcaccaacgt ccgtggcgcc ggtgtcgcct ggacggcggc cttcggtcgt atcggcggca
    36601 tcttcgggcc gatcttcggc gccttcctgg cggtcacgtt cgccggcgag ctgcacagca
    36661 tcttctacgt gctcggcggc atcagcctcg tcggcctggc cctcaccctg atcattccga
    36721 agagcaaggc ggcggcggtt gcacaggcgg tggcgccgac cacggcggct gcggcggccg
    36781 tgcccgccgg caagtcccgt ctctacggca ccatcatggc cgtggtcgat cacaccggaa
    36841 acaggctgac gcgcgagcgt gtctccgagt tcgtctccct gacgggctcg aaggtgcacc
    36901 tggtgtatct cgcgccggag cacgtcctgt ccgccgaggt cgatcagacc gacaaggcca
    36961 tcgacccggc gcacgtggcg aacctgcagg agtatgtgaa cgaggtgaac gcgcagggtg
    37021 tcccggcgga gggccagatc ctgacctcca cgctgttcgg tcgcggcagg gcggtggtcg
    37081 acctggccga gcagctgcgc tgcgacctga tcatcctcaa caccgaggag ggcggccagc
    37141 gtgccaaggc cgagctcgcg caggaggtgg ccggacagaa cccgaagatg gcggtgctga
    37201 tcgcgcgctc gacgcagtaa gacgctgccg caccacgaag cccgccccga ccgctacggt
    37261 cggggcgggc ttttttgtgc gcggcgcaac cgcgcctcgc acccggggtg ggcaataggt
    37321 gtatgagata ttgtgcattc gaaatgtgtt catgcgcaaa gatgtgcgat atagtgcagt
    37381 tttggcgcct gggtcacagg tgcctgcgcc attgcggcgc acgaacatcg ctctcttcgc
    37441 tccgtgatgg ggcgggatgc gggtcgcgcg ccgtgatcgt gagcgtctgc gcgatcttcg
    37501 gtcgcaatgc ccgcagcaac tcccacaacc ccaacgcaag cgccaggaat ccatcgtgag
    37561 aaaagtagat gtgcatcaaa caatcgacga ggcgaagttc aacaaatttc acctgaacgt
    37621 gctgttctgg tgtgcgctcg tcatcatctt cgacggctac gacctggtga tctacggggt
    37681 cgtgctgccg gtgctgatga aggagtggga cctcacgccg ctgcaggcgg gctcgctcgg
    37741 cagcgcggcg ctgttcggca tgatgttcgg cgcgctgatc ttcgggccgc tgtccgaccg
    37801 tatcggccgc aagaaggtga tcatgatcac ggtgatcatc ttcagcggcg tgaccttcct
    37861 caatggcttc gccgagacgc ccacccagtt cgccgcgatg cgcttcatcg ccggcctcgg
    37921 catcggcggc gtgatgccca acgtggtcgc gctgatgacc gagtacatgc ccaggaagat
    37981 ccgcagcacc atggtcgcgg tgatgttcag cggctactcg gtcggcggca tgacctcggc
    38041 cttcctcggc atgtacctga tgcccagcca cggctggcag tcggtgttct tcgtcgccgg
    38101 catcccgctg ctgctgctgc cgctgatctg gatcttcctc cccgaggccg tcggcttcct
    38161 gctcaaggaa ggtcgcaagg aagaggccgg ccgcctgctc gcccgcgtcg agccgtccta
    38221 ctcgcccgcg gccggcgaca acttccacct ggtcaccggc cagggcagga gcgtcgccct
    38281 ggtcgcgctg ttccagaacg gtcgcctggt cagcacgctg atgttctggg tcgccttctt
    38341 cagctgcctg ctgatggtgt acgcgctcgg ctcctggctg ccgaagctga tgaacaaggc
    38401 cggctacgag ctcggctcca gcctgagctt cctgctggtg ctcaacttcg gtgcgatctt
    38461 cggcgcgatc ggcggcggct ggctggcgga ccgcttccac ctgcgccggg tgctgaccat
    38521 catgttcgtg atcgcggcgc tgtcgatcag cgcgctcgcg atcaagaacg acatgatcgt
    38581 cctctacctg ctggtggcga tcgccggtgc gaccacgatc ggctcgcaga tcctgctcta
    38641 cgcctacgtc gcgcagtact atcccctggc gatccgctcc accggcatcg gctgggcctc
    38701 gggcgtgggg cgcctggggg cgatttccgg gccgatgctg ggcggtgcgc tgatctcgct
    38761 gccgctggag atgaacttcc tcgccttcgc catcccgggt gcggtcgccg gcctggcgat
    38821 ctccatggtc gggcgcagcg cggtgcatgc gccggaagag gagaagaacc tcgccttcgt
    38881 gggcgagacc ggcaagctcg aggtggattg aggcgattcc gggccattgg caaccgtcgg
    38941 cgcaccggat cgatgcggga acggccggtg ccgttcccgc attcttgcgt tcgggtgaat
    39001 gtggcgcggg aacagcggcc cgattggcgc tagcattgaa gtcccgccca ggagacccgc
    39061 catggagctc gaagcctacg tccgcgcctt gcccaaggcc gagctgcacc tgcatatcga
    39121 gggcacgctc gagcccgaaa tgatgttcgc gctcgcgcgc cgcaacggcg tggcgctgcc
    39181 ctgggcgagc gtcgaggcgg tgcgcgccgc ctacgccttc accgatctgc agagctttct
    39241 cgacctctac tacgccggcg cggcggtgct ggtccgcgag caggacttct tcgagctcgc
    39301 gctcgcctac ttcgagcgcg cacacgccga cggcgtggtc catgccgagc tcttcttcga
    39361 cccgcagacg cataccgcgc gcggggtggc gctcgagacc gtgctcgacg gcctcgagcg
    39421 cgcctgcgtc gaggcgcgct cgcgctgggg catcggctcg cgcctgatcc tgtgcttcct
    39481 gcgccatctc agcgaggagg agggcttcgc cacgctgcag caggctctgc cgcacctgtc
    39541 gcgcatcgac ggcgtcggcc tcgactcgtc cgagcgcggg catccgccgg ccaagttcgc
    39601 ccgcctgttc gcgcgctgcc gcgagctcgg cctgcatgtg gtcgcccatg ccggcgagga
    39661 ggggccgccg gcctacatcg tcgatgcgct cgacctgctg aaggccgagc gcatcgacca
    39721 cggcgtgcgc tgcaccgagg atccggcgct ggtcggacgc ctcgtgcgcg agcaggtacc
    39781 gctcaccgtg tgcccgctct ccaacgtcaa gctgtgcgtg ttccccgacc tcgcccggca
    39841 caacctcggg cagctgttcg ccgcggggct caaggtgacg atcaattccg acgacccggc
    39901 ctatttcggc ggctacgtgg cgaagaatta tgtcgacacc gcgcgcgcgc tcggcctcgg
    39961 ccgtgccgag ctgcgtcgca tcgcgcgcaa cagcctggag gcgagctttg tgtccgccgc
    40021 cgaacgcgcg ccctggctcg cccggctcga cgcgctcggg gaggactgcg agggagaggg
    40081 cggcgcggca tgacgccatc gactgccgca gagcgccggg cgctgcgtga ccgcctcacc
    40141 gcggtgccgc tgttcgccgg cctgcccgcc gtgcagctgg ccgagctcgc cgccgcggcg
    40201 cgcctgctcg agctgccggc gcgcaccctg gtgcatggct gcggcgagcc cttcgacgag
    40261 gcatacgtgt tgtgcagcgg gacggtggtg cgctttcgcg agctcgacgg cgacgcgcgc
    40321 aaggtggtcg agctggtgca cacccctcag atgctcggct gcggcgagtt cttcggtgcc
    40381 agccgtcatg aatcgggctg cgagacggca agcgcctgcg cgctgatcgc gctcgacgcc
    40441 ggcgtgctgc gcgcggtggc cgagcgcagc ctggtgctga cccggcgcat gctgcaggcg
    40501 gtggcggcgc ggctgtgcga ggtcgagttc gacgtcgccg ggcgccatgc cagccgcacc
    40561 agcgcgcagc gcatcctcga ttatctggtc gagctcgccg gcggcagcct ggcactcgcc
    40621 ggcgagacca cggtggagct ggcctcgacc aagaaggtgc tcgccgcgcg catcggcatc
    40681 acccccgagg ccttctcgcg cagtctgcgc gagcttgccg acaagggcgt gatcgtggtc
    40741 gacaagcgcc tgatccacat ccagaacgcc gccaccctgg acaccggtgc gggcgagcag
    40801 ccgcagcgcc tgagcttctc gcgccgcaac aagggtgcgc gccgcgacga gacgatgtcg
    40861 gtcggtgagc tgatcaacct gtgcggccgc ctgcgcctgc aggtgcagcg tctggcgatc
    40921 gactgggtgc tgatcgggca tggcgtggcg ccggtcgaga tgcagctgcg cctgcgccag
    40981 gacatcgccg agttcgagcg cagcctcgcg gtcctgcagg ccgccagcct cgcccccgag
    41041 ctcggcgagg cgctggagga cgtgggccgg gtgtgggcgg attttcgcga tgcgctgggt
    41101 gcggccgagc gcccgccggc gggcgcggcg cgggtgctgc agctgagcga ggacttcatc
    41161 accgcggcgg attttctcaa cgccgtggcc ggcggcttcg ccgcctccgg cagcctgtac
    41221 atggtcaacg tcgccggacg caaccgcatg ttgtcgcagc gggtggcgaa gttcttcctc
    41281 ttcgagggct tcgagggctg cccagacccg caggcgcaga tcgccgcttc gtgccgggcc
    41341 ttcgagcaca acctgcagcg cttgcgcgag aatgccggcg agctgcccga gctggtggcg
    41401 cagatcgacg ccacggccgc cctgtgggcg cgcttcatcg ccgcgctcga ccccgcgatc
    41461 cgccagcctc atcgcatcgg ccgggtgcgc gcggtgctcg acgaggggga gcgcctgttg
    41521 cgctacacca acaccctggt caagctcttc gagcgcctcg cgcactgaaa tccggccgct
    41581 gattccgcgc cccgcctgtc cggcacgggg cgcccttttc ccctcgatcc gtggagcaac
    41641 ccatgagcgc agcctccagc ccctcccacc cgacgacgcc gcccgccacc gagccggcgt
    41701 tgcgcctgat gccgatgccg gccgacctca actccgccgg cgacgtgttc ggcggttggg
    41761 tcatggcgat ggtcgacatc gccggcgccc tgcccgcgat ccgccgcgct cgctcgcacg
    41821 tggtcacgat ggcggtgaat tccttcgtct tcaggcagcc gatctcggtc ggcgacatcg
    41881 tcagcttcca cgccgacgtg gtcgcggtgg gcaagtcctc gatcacggtc gacgtgcacg
    41941 tgtttgccga gcgccatccg gcggatccgg tggtggtcaa ggtgaccgag gcgcgcatga
    42001 cttacgtcgc cgtcgaccgc gcgggctgca agcgcatggt gggcccggag tcgaccctgc
    42061 cccccggcgg cgagcgtccg cccctggaca aggagctcgt gctgcgggtc gtgcccatgc
    42121 cggccgacct caacccctcg ggcgacgtgt tcggtggctg gatcatgtcg atggtggata
    42181 tcgccggcgc ggtgccggcg atccgcgtcg cgcgctccaa ggtcgccacc gtggcggtgg
    42241 actccttcgt cttcaagcag ccggtgtcga ccggcgacat cgtcagcttc cacgccgagg
    42301 tggtgcgcgt ggggcgcacc tcgatcaccg tggatgtcga ggtctacgcc cagcgccacc
    42361 cggagaaccc ggtggtggtg aaggtgaccg aggcgcgcct gacctatgtc gcggtggacg
    42421 aattccgcgc caagcgcacg atcggggcgg gctgagcccg ctttcagccg agctggccgc
    42481 gcacccagcg cacgatgtcg gccgcgctca tcgcgccggc ctggcgggcg atctcgcggc
    42541 cgccgcgaaa cagcgccagg gtggggatgc tgcggatgcc gaagcgtgcc gccaaggcct
    42601 ggtgggcttc ggtgtcgagc ttggcgaggc gtagctgcgg ttcgagcatg cccgcggcct
    42661 gcgcgaaggc gggcgccatc gtgcgacagg ggccgcacca cggcgcccag aagtccacca
    42721 gcaccgggac gtcgctgcgg ccgacatggc ggtcgaagtt cgccgcgtcg agctccaccg
    42781 ggtgggcggt gaacagcggc ttgccgcacc tgccgcagtt cggtgcttcg gcgaggcggg
    42841 tggcggggac acggttgacg gcgtcgcagt gagggcagac gaggtgcagg gcgtcggaca
    42901 tggcggggct cccgggcatt tcggattctc cggatgtcga ggcgaagcgg cgggattcaa
    42961 gagggccggt gtccgtcgat gcggctgggg gtgggggcgg catgtggccg cgatccggcg
    43021 gataatgccg gcatctcgtt gtgaaggaga agcgccgatg aagctgcccc gaatcgtggt
    43081 gatgcccggt agccgccggc gcgaggcctt gtcgcgtcgc gtggcgcagg cgtgcgcgca
    43141 ggccgtgcgg gaggcggggg ccgaggtcga gctcgtcgat ctggacgatt accccgcgcc
    43201 gctctacgac ggcgacctcg aagccgcctc cggcctgccc gagggcatcg tgcgcctgca
    43261 gcgggtgctg catgcgagcg acggcctgct gctggtcaat cccgagtaca acggctcgct
    43321 cacgcccttg ctcaagaaca ccctcgactg gtgctcgcgg cccaacccgg ccgacgccga
    43381 gcgctccggc ggcaaggtgt atgccgggcg ggcggcggcg gtggtgggaa cctcgccggg
    43441 cgcgctcggt ggcatgcggg tgctgttcca cgtgcgcgac gtgctcggct acctcggcat
    43501 gcaggtcatt ccgcagcagg tcgcggtggg ccaggccggc gaggcggtcg gcgccgacgg
    43561 acgcctgctc gatgccgccc ggcagggcat gctggagggg cttgcggagg cgttggtgga
    43621 cacggcgcgg cgcctgcgcg cctgagggct tcagtccgtc gtcccgcccg tcatcgcggt
    43681 ggcgggcgtc gcacgcaggc cgcgcaccgc tgcgcgggcc tgctcgaaaa cgcgctcggc
    43741 ctcttcgcga tcgaaatccg tggcgtagcg cttcttgacc ccctgctcgt tgagcagcag
    43801 gtgggcggcg ggtcggacgc cgcgctcggc caggctgctg cgcgtgcatt cgagcgcgca
    43861 gccgtcgagc gcgaggatcg gtcgcccgga gcgggcgatg ttcaccagat gggggacgtt
    43921 gccgccgacc ccggcgatgc aggacatctc ggcctcgccc tcgcgatcca ggcgcagcgc
    43981 cacgtggttg gccatctgcg ctgcgctgga gcagcccgag caggaataga cgagcggcag
    44041 gttcttcttg cggccggcca tggcggggtc ctcgggcggg catgttggga cgaatagttt
    44101 actatgctgc ggcagttcac ccggtggacc gtgttcgggg tgaattgacc gctatcaatc
    44161 aacttgtgag ggtttgccgc tagtgtgcaa tgcattcgcg gagacgctct gcgacggacc
    44221 acgacaaatc ggagaatacg catgaccaag tttcccttcc tgatcgccgc tgcggcgtcc
    44281 gttctgctgg ctgcctgtgg caactccggt agcggcactc ccgcggcgcc ggccaaggcc
    44341 cccgcggctc cggcagcggc tgcggccccc gctgcgccgg ccgcagctcc cgctgccgcc
    44401 cctgttgcgg ccgcgcccgc cgccgctccg gccggtgccg cgggcgaggg cacctacaag
    44461 aaggtttgtg cgctttgcca cgcatccggt gttgccgggg ctccgatccc gggcaacaag
    44521 gccgactggg agccgcgcgt cgcacagggc aaggacacgc tctacaagca cgcgatcgag
    44581 ggcttcaccg gctcgaaggg catgatgccc ccgcgcggtg gcggcgcttc cttgaccgac
    44641 gatgaagtca aggctgcggt cgacttcatg gttggcaagt tgaagtaagc cggactgcgg
    44701 cagtccgctg cggcggacga cgatgtgttt ttcgcggggc ctgcgggccc cgctttccat
    44761 cgcgcgccgg tgcgctcagt gcatgtgatg ggcgtggttg gcgtcgcgca ggctggtgac
    44821 gacgctggcg tccacctgct cgaaggtcag tgcgcggccg ccttgctcgg cgatgaaggc
    44881 ctgcgctgcg gcggtgtcgg cgaaggccgg cagatcgggg ccgcgcatcg gcccgcgcgc
    44941 gcgcgagtcg cgcacgaaga cggcgtggcg ggcgggaagc caggtgccgt tgcggaagtc
    45001 ggcgacgtac atcgccgcgg cctcggcggc ggtgcgcgtg ctgccgaagc ttgccggttt
    45061 gtccaggaac atgaacaggt cgaccggcga gtcgaagaag tgcgccgcgc cgtcctcgaa
    45121 gacgatctgc gccgcccagc gcgggaagcg tgccggatac atgccgcata cggggcagcg
    45181 tgcgtccgcg gggatgggcc tggcgtcgta tttgcccagg ccgctcgcag ggtcgtacgg
    45241 atgggcctgc gccgcccgcg tcggggtgac gacgcagatg tcttccgcgg gcggctcgaa
    45301 gctgcggccg gcgcgcgtcg tgtagtccca cagcagcgcg ctgcccgcgg cgagcgagac
    45361 ggcggcgccg gcgagcagga gccggcggcg accgcgtacg atgctcacgt gccgaaatcc
    45421 gcgagttgcg gatcgcgacc gaggacgtcg atcacccgcg cgaggttttc ctgcggactg
    45481 cgccgcggat cggtgagcgg atcggccgcg acgctgccgt cggcccgatg cagatggttg
    45541 ggtgtggtct cgagttgcgg atgaacgggc gcggtgtcga tgcccagttc gactccgtcg
    45601 atgtccgcgc tcgtgcgcca gcgcagcgag taggcatcgg gtgcggcgta gcgcacggtg
    45661 aggaccactc cattcctgag caccaggttc aatgcatcct gcgacaaccg tgcgggggca
    45721 agcagtgcac cttcgtgtgc gcgctcgacg tagtcgagca gttcggtgtg caggctcatc
    45781 agcggttgat ctcccgcagg cgctcgccca cgacctggcg tgcggcctcg tgggtatcgg
    45841 ccaggatgcg cgcggcgagg tagtgttcgt agccgggatg ttcgtcgacc gcgccggcgg
    45901 cgatgcgggc gagcaactcg gcttcgtcgt tcgcgccgat ggcggcgagg accttgcggc
    45961 ggttttcgcg cgcctcgtag atgagccggg cgatctgctc gagctcgagg gcctgctcgg
    46021 gggggatggc gtcgaagatc ggattcatcg tggtgcctgt cctgcttaca tggactgatc
    46081 gtggagggcg ccgccgtcga ggatcgccat gtcgggcgtg acctcgtcga agcgcagcac
    46141 cttgccgccg ttctccgtgg cgaaggcctt ggcgtcggct tcgttggcga acgaggccgc
    46201 ggtggggccc atcgcaccca tgcgccggga gccgaacacg taccaggcct tcttcgcatc
    46261 gatccagtgc ccctgggggg cgttccacga ggccttggcc atgtcctgca cgaagatcgc
    46321 ggtgaccagg cgggcctgct ccgggttcag gtatacgttg aacatctcga tggtgtcgca
    46381 gaaccagtcg gggcgctcca cgccggcgta gtgcatctgg accttggagc cggggtagtc
    46441 ggccagcagc atgccgtcga gcgcgcaact ggtggcgcgg tcgatctcga gcggcgtcac
    46501 ggtgccgctg ccggctccct gcttgccgct gccgcaggcc gcgagcagcc cggcggcaac
    46561 gccggcgatc aggaagcggc gacggctcgg gcaggcgtga tgtgtgcaca tggggaaatc
    46621 actcgatacg gttcgtttgg aaggagtgtt cagcggcgga agcgccacgt cgcgatcgcc
    46681 agcggacccg cgatccatgc cgccatgacc agcccgagca gccagggccg cgccagcgcg
    46741 ggcgggaaga cggtcgacag gccgtagagc gtgcggacgt cctccatcga gaagatgttc
    46801 aggatgcgga agacgtcggc cgggttgaac agcaaggcgt agggcagcgc ttcgccgccc
    46861 caccggccgg cggtgacgac cagcacgccg agcaccagga ggtcgaagat cagcacgaag
    46921 aagaaccagg tggcgatcgc caggccggag gcgcgggtac ggtcggcggc gaataccgaa
    46981 agcatcaccg cgatgctcag gaaggcgcag ccgagcagaa ccgagctgag catgaagccg
    47041 aagtaatgga acagcgagcc gaggtcgagc tgcgccgaca gcaccacgcc gacgaggccg
    47101 aagccggcga cggtggaaaa ggtgagcgcg cccgcgaggc cgaggtattt gccgagcagg
    47161 agttcgaggc gggtgatcgg catcgacagc agcaggtcga gcgagccgcg ttcgcgctcg
    47221 ccgacgatgg cgtcgaagcc gagcaccagc gcgatcaggg gaatcaggta gatgaccagg
    47281 ctgaccaggc tggcgatggt gacctcgatc gaacggaagc ccacggcgcc ctgctgcgcc
    47341 gagccgaagt aggcgatcga cagcgcgaac acggtgaaca ccagcgccac ggccaggacc
    47401 cagcggttgc ggatgcggtc ccagaactcc ttgccggcga tggtggcgat ctgtgaaaac
    47461 tccatggtcc gcctctcagt ccgaaaagcc gaagaacacg tcttcgagcg aaggctcgcg
    47521 caccgtgagg tcgcggacct tgccattcag ggtggccaag gcggcgatca ccgccatctt
    47581 ggcatcgcgc gcgcactgca ccgcgacatg gccgtcgcgg gcttcgatgg cgcctaccgg
    47641 caggccgccg agggcgttgc gcaccgcctc gaaatcctcg ggcgcgacgc gcacgtcgaa
    47701 ccacagcggc agatccatct gctcgcgcag ggcctgcacc gtgccggtgg cctgcacctt
    47761 gcccgcggcc atgatcgcaa ggcggtccac gcgttcctgg atctcggcga ggatgtgcga
    47821 ggtgatcacc atggtgactc cctcgctctt caattgccgc aggatggcgt agaagccgcg
    47881 gatcgcctcc gggtccagac cggtcgtcgg ctcgtcgagg aacaggatgc gcggcttgcc
    47941 gaggagggcc tgggcgaagc cgaggcgctg gcgcatgccc ttggagtatt cgcgcacgcg
    48001 acgcttggcc gcgtgcatca ggccgacacg ctcgagcact gcggcgttgt tcgccgtcga
    48061 gacgcccttg agacgggcga agaagtcgag cgtttcggtg ccggtgaggt tgtcgtagag
    48121 cacgacgttc tcgggcaggt agccgacctt gcgtcgcacc gtgcggaagt cgctgcccgg
    48181 caccggcgcg ccgtcgatgc ggatctcgcc ggcggtgagc gggatcagtc ccagcatcat
    48241 cttgaacatg gtgctcttgc ccgcgccgtt gtggccgatg aggccgaaca gctcgccgct
    48301 gcggacctcc aggtccacgc catccaccgc gtggatgggg ccgtagtgct tgcgaacgcc
    48361 gcgggcgacg atcaccggtg cgccgggatc attctgagcg ggggaaatgt ctgccacgcc
    48421 attggctcca gtcaggtttg ttcggttcca tgcgcggcct ggggtccacg atgctgggcg
    48481 cacgcagcag gggaaactgc tgggccacca tgcgcaaggt ctggatggct gggctgccga
    48541 gcagcagctt catcatcggg tgacgccagg tcaggcggtc gaccaggtcg ttggcctcgt
    48601 accgcacgtc gcccaggccg tcgccgttgc ggtcccagcc gaggtagttg ctccagtggt
    48661 tgccttcctt ctcgccccag atcatgtcgt gcgcggccac gtagcggatc tgctcgcggt
    48721 tgccgatgaa gtcgttgcgc tccaccttgt tgttcttgga ccccgcccac aggtggatcc
    48781 cgaccacgtt gtcgatgatc tggttgccgc gcagctcgtt gtactcggca tcgtagatga
    48841 agaagccgcg ctggttgccg gcgaccacgt tgttctcgat cacggcgtcc tggatcgagc
    48901 gcagcatgat gccgtggtcc gagttgcccc aggcgcggtt gttgcgcacc acctggtcgc
    48961 gcacttccat cagcgcgaga ccgccgcggt tgtaccaggt ctcgttgtcc tcccagacgt
    49021 tgcggtagga ggtcatgtag tgcgtgccgt agcggctgtg atgcagcttg ttgccgcgga
    49081 agagcgcgtc gtgggagacg tccacgtaga tcgcgtcgcg cacgaagctg atgttgttgc
    49141 cgatgatgcg cgcgcgctgg gtgttgtaga gctggatgcc gttgccgcgc ttgggcgagt
    49201 cgaaatcgcg cttgccggtg atgaggttgt tctcgatcag gacgtcgttg gccttctcga
    49261 tccacagccc gaacagggtg taggagaagt cgcaattgcg cacgaccgcc ttgtgcgagc
    49321 cggggtagat atagacgccg gcgtgctggt agagggtgtc gccgcccgag ttggcgacga
    49381 tgagcccctc gatggtgacg ccctccgcct ttatgctgat ggtgttttcc ttcagctcgc
    49441 cgtcgatcgt ggggcgctcg atgccgcgca gggtcagcgg cttgtcgacc agcaactggc
    49501 cggcgtagcg tccgcgctcg atttcgatca cgtcgcccgg cgctgcgcgg tcgatcgctg
    49561 cctggatgga ctcgcccggc gatacccgcc acggcgtggc tgcagccggc gtggccagtg
    49621 cggcgagcag ggctgcggca gccaggaggc gaggaagacg gtggagggag cgcattgcgg
    49681 gggggcgttt gacagtgggg cagagtatgc gacgcaatgt cgcagcgaga cttgatctag
    49741 gtgaagtgcc cggcggcggc gggcccgcgg aaaacggcgc gccgccccgg gggcggcgcg
    49801 caatgtaacc cgggcgggcc ccggatgtca gacctgcacc aggcccatgc tcttcagcac
    49861 gatgaacagg gcggcgccga tcagcaggtt cttgaagccc acgtagcaga tcatgtccat
    49921 gatgctggcg cggcggtagc ggctgcccca ccacggaccg tccttcacgt agggcttgga
    49981 acccaggcgc accgccacct cgaacacgct ccagccgaac caccagccga tgatggcacc
    50041 gggcgtcatg ttgcccatcg ctgccaggat ccaggccacg ctcgcggccg cggcgagcgc
    50101 caggccggcg gcctggacga cgcgctggcg ctggaagccg ttgcggctcc acggccacag
    50161 gtgatcccag atctcggcag gcaggcggct ggcggagccg cggctggcat agggcggctc
    50221 cacctgctcg gtcagaatgc gcggatcgac catgatcgaa tcctcctcga atgcagtgtc
    50281 ttgatcaggc ccgcggcgct gcgggcgcca ccggcttgat cgggatgtag taaccgtcct
    50341 tgccgatcgg ggtcagcggc aggccgtcct tggtgcgacg cttgcgttcc tgtgccagcg
    50401 gcgggcaggc gtggtcgtcg gtgtagagga ccatgcagtc caggcagagc atgcattcgc
    50461 gatggtcgat gcgacctttc tcgtcgatcg ccagcgagcc gcagccgacc gcgcaggcct
    50521 tgcagctgtc gcattcctgc ttgcggcgca ggccgaacca gcggaaggtc gagggcatgg
    50581 ccagcgaggc gccgagcggg cacaggtatt tgcagaacgg ccgctccatg aagatcgaca
    50641 ggccgagcag gccggcggcg aacagcgtgt agggccagct gcggttgaac aggccgacca
    50701 ggaaggtggt cttgaagggt tcgacctccg cgagcttctc ggccaggccc atcgagaaca
    50761 tcgataccgc gagcaggccg aagaacaccg cgtacttcac ccacttcagg cggttgtgcc
    50821 acttcatcgg cagcgcgaac tggaagcgct tgaggccgat cgcgccgccc accttgtaca
    50881 gcagttccga cagcgagccg aacgggcaca gccagccgca gaacaggccg cggccccaaa
    50941 cgaacacggt gacgatgatg aagatccaga acaggaagat gaacggatcg gtgaggaaca
    51001 gctcccattc ccacttgaac agcagcgagt ggaaccaggt cagcacctgc gtgatcgagg
    51061 gctgggcgag caggccgaag ccgacaaaac cgatgctgat gatccacgcg ctgtacttga
    51121 aggcgttgac cggccacttg ttcttgcgcg tcgagcggcg ggtcagttca tcgcgcttgg
    51181 catagaccac cgccacggcg acgagcaagg ccgagaacag gacgatctcg agcgcgcgcg
    51241 tcttccacac gcggacccag ggcgcatcgg gcttcttgac ctcgggccgg ccaccctcga
    51301 tataggcgtc gggcagccag tattccctgt cgaagttgat gaaggtgcgc gcaccggtct
    51361 cgcggtcgat gcggttgccg aggaagatga acttccatgg gaaggcgccg gagaacgagg
    51421 atgagcgcac gatgaagatc gccgactcgg tgaaggacgg cgcgcctggt gcggaaatcc
    51481 catagaggtt gtagtagtcg aggtcgcgga aggtgaacac gtcctgcccc tggcgaagct
    51541 ggacacggtc gtagatgccc ccgcgcacga agcccgagcc cttgaacgac tccctgcctg
    51601 cggtgcggat gacgaaaagc gcgtgttcgc cctccttgat gcgtgccatc aggtcgcgcc
    51661 agccggtttc tcccatgatc gcgcggccga cctcgggatg gttcagatag ccgtaccaga
    51721 ggtcgatgaa cgggtccttg ccacgctcga ggccgacctg ctcgggcatc acggtcaggc
    51781 gttgcaccac gccttccttg accagtgtcg cccagtcgag cttgcggccg ctgtcgacga
    51841 acctggcctg agggcggacg gtcttttcaa ggatgccgac ctggcgcccg actgcagcgc
    51901 ccgacatcat catcacctgg ttctgcgcga tgaccgtgac ggtggcgccg gagatggcat
    51961 cgaggccgat gatctcttcc tcggggcgcg agcggccgat ctcgatgttg gcgccaacga
    52021 acttgcccag gtattggttg ttgaacttga ccagcgcgga ttcggggatg ccgagcagca
    52081 ggatcggttc ggagtgcttg aggatcttga tgccggcgaa gttgccttgc ggggtaagcc
    52141 cgatcagcgt gatcacgggc ttgccggagt aggccggggt gtcggtgacg tcggtcgata
    52201 gcaccacgta gcccaccagc ttgcgttggc cgttttcctc ggcgtagccc tcgacgtagg
    52261 gcggctgacc cttgcgctcc gagaagctgg tcgcgccggg caggacctcc ttgcagggca
    52321 cgtagtcgca cagtgcgggt gcggtgttga cctcgggcgg taacttggcc tcgtaggcac
    52381 tttcggcgga ggcgctctgc ggcaggaggg tcgtgacggc gagcagggcc gccatcgcaa
    52441 tgccccgcag ggcggagagg ggcttcatga tgcgctccat aagtaaatca cgatggagtt
    52501 tagggggtgg ggtccggcat gtatttgtcc agcgtcaaat tgccggtggg cacgcctcga
    52561 aacgcgagcg gactgcagaa aacaatcgag gggcttgcgc ccctcgatct tcagacggca
    52621 ccgcagtcaa gcggcgcggc gcgctccggg attacggacg cacgagcatg cgcgtacgca
    52681 tctccaggtg cagcgcgtgg cagaagtgcg tgcagtagca ccagaacacg cccggcttgt
    52741 cggccttgaa ggtgacggac ttcgtctcca gcgggttcac cacgaagttg atgtcgtact
    52801 tcggaatcgc gaagccgtgc gacaggtctt ccaccttgtc gaggttggtg aggatgaggg
    52861 tgacctcgtc gcccaccttg agttcgaact cgcgcaggct ataggccggt gcctgcgagg
    52921 cgatcctcac cgtgaccttg ttgccgtcgc ggaacacgcc cgattccttc gcatccttga
    52981 tcgccagcgg gtgtgcgtcc agcacgccga cctgacgggt cttgatgagt tcgcgcttga
    53041 cgatgatgaa gtcgtgcggc tccggatgca ccgggtggtc ggccagcagc accatcttct
    53101 cgccacggat gtcgatcagc tgctcgtttt ccgggtgcat cggacccacc ggcaggaagc
    53161 ggtccttgga gaacttgcag cccaccgcca ggaactggcc gctggcttcc tt
//
LOCUS       DAOQBS010000005        53186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_270_length_53186_cov_15.963562, whole genome shotgun sequence.
ACCESSION   DAOQBS010000005 DAOQBS010000000
VERSION     DAOQBS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 53186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53186
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_270_length_53186_cov_15.963562"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKN25_01130"
     CDS             complement(<1..310)
                     /locus_tag="PKN25_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV89526.1"
                     /translation="MHKTLTAFSAAKTQPSFDFPRLERLVAGNPQRATWAHFERDGMS
                     AGLWGCEPGAWRIAFADDTDEFFHVLSGRIRITDEGGLAREFGPGEACVIPAGFNGVF
                     E"
     gene            557..1090
                     /locus_tag="PKN25_01135"
     CDS             557..1090
                     /locus_tag="PKN25_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89527.1"
                     /translation="MNAPFLAFLTWPQRWLLLTVLLALSALLLFGRIASLDEFIKARG
                     EGGIMALQFAGSADRAAHLLVAWRGLEDLLRSQTRWDFLFLLVYPAVLSLACAMLAEA
                     RGNPLPTIGIFVAWLALAAIPLDAIENLATLRMIDHGASEPLARWITVCATMKFAIVL
                     TAAGYLLVAGIATLFQR"
     gene            complement(1213..1638)
                     /locus_tag="PKN25_01140"
     CDS             complement(1213..1638)
                     /locus_tag="PKN25_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNV89528.1"
                     /translation="MDIEQFMQGYKKAWEERDEAQFCALFAEDGEYHNTPFAVQRGHA
                     QLAAYWQRVKLQEDVQVRYEILATSPAGGIAHWHVTYQVASEELFRIWAQSTGTNLVA
                     RKPGDPLPRMVLDGVLKAEFGSGVCTRCAIWWHSMPEPV"
     gene            1745..2329
                     /locus_tag="PKN25_01145"
     CDS             1745..2329
                     /locus_tag="PKN25_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HNV89529.1"
                     /translation="MLGNSERERVHTRRIVCEAFRRRDGLWEIEATVIDEKGEDMPFR
                     SRPMVRPGQSLHDITIAVLIDDDAVIHDVAARMQTVPWAACPESQAAYRRLIGLQIGA
                     GFMREVRERVGGVEGCTHLTDLLVQVGNTYTQAIWPTLIARQFAAEPDPRKWTDRRAV
                     GFVGGCAAWRQDGDTLRQEYPELAAEFVAKRDET"
     gene            complement(2367..2819)
                     /locus_tag="PKN25_01150"
     CDS             complement(2367..2819)
                     /locus_tag="PKN25_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89530.1"
                     /translation="MSIQGLGSSASTASWVGAQLSREQARRSAEQLEAKAAALSAEAA
                     SARDEADTAQRRADELQTQAGQARTRAEIGKQAVASVRGMDRVAQTLSAAVGRLADAA
                     EAGPSQGLGLQESGRIPAPTASSDLYTPAAGVRPALSVQPGQVVDLKV"
     gene            complement(2861..3262)
                     /locus_tag="PKN25_01155"
     CDS             complement(2861..3262)
                     /locus_tag="PKN25_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89531.1"
                     /translation="MNPVSREVMRPTDPRQVASAEREQQGARAQDAARDPSARQVAQP
                     SAVVTLSAAARGEGVVQPPAQGGSVEARAIEQARAAAANGDLRSNYSAQRALASYEAM
                     SQVGANNAAARPSQPEQPTAPGQREPGVLRT"
     gene            3661..4329
                     /locus_tag="PKN25_01160"
     CDS             3661..4329
                     /locus_tag="PKN25_01160"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV89532.1"
                     /translation="MNRNWTGFKVLAPAALALVSQAAAADVLLTLGEQDFTNGSTPGT
                     ADFLTAGGNESAPFDGVFSGADTASNFSTSWTFASYGSQPTILGASLTLGIYDHESAA
                     PGNQVAAFTLNGFDLTSAMSTLFEASGGARSEYDVYTLTLPSSVFATLGTGTATFALA
                     LQGPGLGLFGETAFNGAALDFSTLRITTTAQPPTNNVPEPATGALLSMALAGFAATRR
                     RRAQ"
     gene            complement(4359..4838)
                     /locus_tag="PKN25_01165"
     CDS             complement(4359..4838)
                     /locus_tag="PKN25_01165"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV89533.1"
                     /translation="MALRAECGNDEAFVFTLFVEARAPGFANIPLEPAQRDALLRQQF
                     TLQTTQYRRSFPAASFSIVEWGAASIGRLYVDRSAPSMHVIDVALLRAWQGRGIGSRL
                     LGELLEEARVAGRAVSLHVERESPAQSLYLRLGFRVLRDKGVYLEMEWRPAPGGRAA"
     gene            complement(4898..5401)
                     /locus_tag="PKN25_01170"
     CDS             complement(4898..5401)
                     /locus_tag="PKN25_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNV89534.1"
                     /translation="MSEPFVGEIRPFGFGYAPRGWATCDGQLLPINQNQALFALLGTM
                     YGGDGRTTFALPDLRGRAAMHIAQAHPQGQSAGTETVSLTVAQVPQHSHAAMASAAPG
                     TQSSPAGKVWAQDSSGNVIFSSAGNAPLAAGAIGPAGAGQAHANMQPYLAVNYCIALQ
                     GIFPSRD"
     gene            complement(5504..6022)
                     /locus_tag="PKN25_01175"
     CDS             complement(5504..6022)
                     /locus_tag="PKN25_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNV89535.1"
                     /translation="MSDPYIGEIRSFGFNFAPRGWAQCNGQLLPIAQNTALFSILGTM
                     YGGDGRTNFALPNLSGAVAMGSGQGSGLTPRSQGERGGSESVTLTLGEMPVHGHAANA
                     NPANGNQPGPGGNFWAQDLGGSKDYADADTVAMAPGAIGIAGEGQPHSNVQPFQVLNF
                     CIALQGIYPPRG"
     gene            complement(6071..6592)
                     /locus_tag="PKN25_01180"
     CDS             complement(6071..6592)
                     /locus_tag="PKN25_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNV89536.1"
                     /translation="MADPFLGEIRAFAGNFAPLGWALCQGQLLPIAQNDALFALIGTT
                     YGGDGQTTFALPNLAGRIPIHQGAGPGLTPRALGEAGGSETVALGVPQLPSHSHVAIG
                     ANSGADRIGPAAAYWSTDPGGNTGAYSTTAGSQMATGAIGAAGGGQAHANMQPFLVLN
                     YIIALEGIFPSQS"
     gene            complement(7009..8142)
                     /locus_tag="PKN25_01185"
     CDS             complement(7009..8142)
                     /locus_tag="PKN25_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNV89537.1"
                     /translation="MILTQEQELIRDSMRAFAQERLAPFAAEWDRNHTFPREALNELA
                     ELGALGMVVPEEWGGAGMDYMSLVLTLEEIAAGDGATSTIVSVQNSLPCGILNRFGSD
                     AQKEAWLKPLARGEKLGCFCLTEPHVGSDASAIRTSAVRDGNEWVLNGVKQFITTGKH
                     ADVAIVFAVTDKAAGKKGITCFLVPANAPGYQVGRIEEKMGQKASDTTQILFEDCRIP
                     ADSVVGKEGEGYRIALSNLEAGRIGIAAQCLGMARAALEAAVKYAQERESFGKPIFEH
                     QAVNFRLADMATQLEAARQLVWHAASLKDAGRPCLKEASMAKLFASEMAERVCSDAIQ
                     VHGGYGYVTDFPVERIYRDVRVCQIYEGASDIQKLVIGRALAG"
     gene            complement(8276..9484)
                     /locus_tag="PKN25_01190"
     CDS             complement(8276..9484)
                     /locus_tag="PKN25_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNV89538.1"
                     /translation="MSDPVVIVSVARTPLGGFQGDLSSLTTPQLGSIAIKAAVERAGV
                     APAQVQEVIMGCVLPAGLGQAPARQAALGAGLPLSAGCTTINKVCGSGMKATMLAHDL
                     ILAGSNEVMVAGGMESMSNAPYLLPKARAGYRLGHGQMFDHMFLDGLEDSYSKENKGR
                     LMGTFAEDCAGHFNFTRQAQDEFAIASTTRAQAAINNGDFSWEVVPVTVASRKGDVVV
                     DKDEQPLKAQIDKIPSLKPAFKKDGTVTPANSSSISDGAAALVLMRKSTAEKLGVKPV
                     ATIIGHATHAQEPALFTTAPVGAMQKVLAKAGWTTEQVDLWEINEAFAVVTMAAMHEM
                     KLPHDKVNVNGGACALGHPIGASGARILVSLIGALRKRGLKRGVASLCIGGGEATAMA
                     IEVDSSVACG"
     gene            complement(9531..10673)
                     /gene="can"
                     /locus_tag="PKN25_01195"
     CDS             complement(9531..10673)
                     /gene="can"
                     /locus_tag="PKN25_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNV89539.1"
                     /translation="MPTRLCALGANDTVELEHVRGFFRQYAAWLGVDLSFQGFADEIA
                     NLPGAYGAADGRLFYAEVDGKPAGCVGIRRFSEGVCEMKRLYVDPAFRGGGVGRKLAL
                     AAIKAARLFGYRRILLDTIPSMRIAVKLYRELGFKEVPACYPSPIEGAIFLTLDLENW
                     SEDEVSNENLFHLFDYNQAWSRQMQQLDPGFFGKLAQLQAPEYLWIGCSDSRVPANQI
                     VGLLPGEVFVHRNIANVIVHTDLNALAVIQYAVDVLQVKHIMVVGHYGCGGVKAALER
                     ARVGLVDLWLRHVQDVHVRHLKAVDGLAPELRHDRLCELNVIEQVANVAQTVVVQDAW
                     RRGQRLTVHGWIYGLQDGLIRDLGMNLSRSDDLMPRYVAALEALGN"
     gene            complement(10733..11200)
                     /locus_tag="PKN25_01200"
     CDS             complement(10733..11200)
                     /locus_tag="PKN25_01200"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNV89540.1"
                     /translation="MRPELPQFQPRDPGYAERVRASFALQQAMRLIGAEIPVVEPGYT
                     EIHLPFKAEISQQHGFVHGGVVGMIADSAAGYAANTLTPADTSVLTVEYKLNLVAPAD
                     GERLVARGEVVKPGRTLIITKAEVFAIRDGKWTLCALMQQTIMAMHGKKERAA"
     gene            11352..12386
                     /locus_tag="PKN25_01205"
     CDS             11352..12386
                     /locus_tag="PKN25_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV89541.1"
                     /translation="MSPPDPTVLEYPFDSLPSPGHSIEIAPGLRWVRMRLPFALDHIN
                     LWLLDEGEALAVVDCGFARDETREAWDAVLAADGRPLRRVVVTHHHPDHIGLATWLAA
                     RDDAAIHMTQGEFFAAQAVWHQLPGYCATDMVEQFRRHGLDEMRRDALLTRGNAYRIG
                     APQVPGRYERMFAGDELRIGAHTWRVIVGFGHAPEHASLYCAELGVLIAGDMLLPRIS
                     TNVSVYAATPHGDALGWFLHSLREFCTLPDDTLVLPSHGKPFKGIRARVEQLVEHHRE
                     RCEVLVAACAMPQSAAALLPTLFPRELDTHQVMFAMGEAIAHLNHLEKRGELSRRVDA
                     DGLIRFTHCP"
     gene            12436..14259
                     /gene="phaC"
                     /locus_tag="PKN25_01210"
     CDS             12436..14259
                     /gene="phaC"
                     /locus_tag="PKN25_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNV89542.1"
                     /translation="MAAPSNETPACDLPDPQEVAKTYAEVARRASHLISDHVHRQLKK
                     GVSTPADELGIAQAFMDMMAKLLANPYKLAQAQMNLVWDYFSLWQHSMMRVAGLSASP
                     VAAPEKSDKRFKDEEWEQHFLFDFIKQSYLIAARHIHDTVSGVDGLDEQTQKKVTFYT
                     RQYIDALSPSNFAMTNPEVFRETVKSHGQNLLKGLNNLLRDVEDGGGNLRVKMTDTSA
                     FELGKNVATTPGKVVFQTEMMQLLQYTPSTEKVLKKPLLIVPPWINKFYILDLREKNS
                     YIKWAVDQGHTVFVISWVNPDERLAQKSFDAYVKEGVIAALDAIERQTGEKEVNAAGY
                     CLGGTLLATTLAYLAAKRQKRILSATFFTTMTDFTDPGELGVFIDEGQVSSLEKKMFE
                     RGYLEGSEMAGTFNMLRANDLIWSFVVNNYLLGKDPFPFDLLYWNSDSTRMPAAMHSF
                     YLRTMYMENRLIEPGGIEIDGTPIDLGKIKVPCYFISTIEDHIAPWKSTYMGARRFGG
                     PTRFVLGGSGHIAGIVNPPAANKYGYWLNPSAKLAETADAWLEGAQQHPGSWWTDWQA
                     WVTAHDSEQTEARDPVNGKLEVLEDAPGSFVKIRIDAKAAA"
     gene            14357..14833
                     /locus_tag="PKN25_01215"
     CDS             14357..14833
                     /locus_tag="PKN25_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV89543.1"
                     /translation="MTEPSSQAPLPPTLVLDTNTVLALWMFHDPALEALRAWIDAGSC
                     RLVSRADALEELRRVLAYAQFGLDPGRQDGLRARYIDRIAVLADDADAGTLPSCRDPD
                     DQKFLEIAGAAGMATLITRDKALLRLARHRLVRERFAILTPERVQHTLAQPSATPA"
     gene            complement(14843..15682)
                     /locus_tag="PKN25_01220"
     CDS             complement(14843..15682)
                     /locus_tag="PKN25_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNV89544.1"
                     /translation="MHSKASQDGCPTWIDAAGRLHSPAGADARIVSLVPSLTELVFDL
                     GLGERLVGRTGFCVHPREALRAVPKVGGTKDVNLARIRELAPTHVLVNMDENRRETVD
                     ALAAFVPHIVVTHPNTPQDNLVLFALLGGVFGAEGAAARLAHELRAVLDEAAALRAAL
                     ADERVLYLIWREPWMTVARDTYIAATLAEVGWSTLPAVEGGETGAARYPAFDWNAPWL
                     AGVERVLLSTEPYRFRAAHLAEVARLAARPALLVDGEMVSWYGSRAARGLRWLMALRR
                     GLA"
     gene            15954..16922
                     /locus_tag="PKN25_01225"
     CDS             15954..16922
                     /locus_tag="PKN25_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNV89545.1"
                     /translation="MKRLTALAAAFAIAGSLGAAPAHAQQKFITIGTGGVTGVYYAAG
                     GAICRLINKDRAQHGVRCSVESTGGSVYNVNTIKAGELDFGVSQSDIQYNALKGEVQF
                     KDGGAFADLRSVFSLHGEPLTVLARKEANIGKMEDFKGKRFNIGNPGSGQRATLEVLL
                     PAMGMSFADFSLASELKPDEHGAALCDNKIDGFAFVVGHPAANIQDPTTTCGAKLVPI
                     TGAGVDKILAQYPYFAPVTIPGGMYANNPDATKTFGVVASIVTSAKVPVESVYIMVKA
                     VFENFEDFKKLHPAFANLQPQDMIKNGMSAPLHEGAVKYYKEKGWM"
     gene            17046..19652
                     /locus_tag="PKN25_01230"
     CDS             17046..19652
                     /locus_tag="PKN25_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HNV89546.1"
                     /translation="MASPEKQEHGFELTDEQLRQIVAEADTGGRKPTGWPARLIVLVA
                     LAWSLMQLWIASPLPFSLGMFVFNDTEARSIHLALAVFLAFLAYPALKRSPRDRIPLG
                     DWVLAAVGAFCMGYLFLFYRELAARPGLPTTTDLAVAVCGLILLLEAARRALGLPMVV
                     LSVVLIGYVFLGPYMPEMIAHRGASLAKGMSHLWLTTEGVFGVALGVSTSFIFLFVLF
                     GSLLETAGAGNYFIKSAISLLGHLRGGPAKAAVVASASTGLISGSSIANVVTTGTFTI
                     PLMKRVGYAPKKAAAVEAAASVDGQIMPPVMGAAAFLMVEYVGIPYTEVIKHAFLPAL
                     ISYIALFYVVHLEALKADIRGLPRRVTKSAGHRLLSFGLTVSGLIVLAGAVYYGMGWI
                     KALAGDASAIIVAVLMFGCYIGLLWYATRHPELHMDDPSSPLTELPETGPTLKTGLYY
                     LLPVVALIWNLMVEELSPALSAFWATLFMIFILVTQRPLKAIFRNTGNVGEATRQGFA
                     DLHTGLVTGARNMIGIGIATATAGIIVGTVTLTGVGLVLTEFVEAVSGGNLMVMLVMV
                     AVICLILGMGLPTTANYIVVSTLMAPVVVEVGAQSGLLVPLIAVHMFVFYFGLMADVT
                     PPVGLASYAAAGIARCDPIAAGVQAFLYSMRTAILPFMFIFNTQLLMIGVDSVWLFAL
                     TVSSAIVANLMFAAATQGWFITRNRVHESILLLLVTFTLFRPGFWMDMLYPPYQELPA
                     SELTRLVEEAPRNDNLRIWVEGISLEGEEVSKGMLLPLGDTAPSARERLGRVGLTLMS
                     LGDEVQVAAVRFGSQSEKLGLEQGYKIARIEVPSVDRPSREWMFVPALALLGFVWFAQ
                     TRRARRQQPTRA"
     gene            complement(19729..20157)
                     /locus_tag="PKN25_01235"
     CDS             complement(19729..20157)
                     /locus_tag="PKN25_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV89547.1"
                     /translation="MSDRGASAVPQAVEAAERRRIQRFLAQRRGQPWFWVMIGDERIE
                     LRDISVDGFAIAAETPPAGGEPFDFVLQREGVPDHIRGRAQAVNFIAASGQAGCRFRA
                     LEGDGAERLRDWLIALVIMNASVRISEKDAAAIVEGPSLI"
     gene            complement(20160..20786)
                     /locus_tag="PKN25_01240"
     CDS             complement(20160..20786)
                     /locus_tag="PKN25_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HNV89548.1"
                     /translation="MNRRDALKLAGLALALPAASVVQANEPFQIIEPAQKADDPSKIE
                     VLEFFHYGCPHCRDFHPLITAWKKKLPADVAFRAVPAVWNNAQLSGLARLFYAAQITG
                     DLAALEPAIFAAVQDDKRPLFNEQQVSEWIAGKVGNAAKFVETYKSFGVGSMVQRADQ
                     LVRAMKIQGVPSMVVGGRYLTSASLTGSHENTLKVADELIARVRKERG"
     gene            complement(20860..21528)
                     /locus_tag="PKN25_01245"
     CDS             complement(20860..21528)
                     /locus_tag="PKN25_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNV89549.1"
                     /translation="MSRDRGPVRKPARAPAKSAGGTLIGIFIGLVFGALIAAGAAWYF
                     TRSNPFQAAPVAPRVQAPAEQGPAALPGKPGDRPVAKQDFEFYRILPQGDNVPSSAAQ
                     VVPVPAPQVQAPPPPVPAQQAPAPVFEASPQPAAVTPPAPQRNERIYLQVGSFENPSE
                     ADNLKARLALSGIQASAQRTQLPDGRIVHRVRVGPFAKPEDMNPMRARLSDAGFNASV
                     SRNQ"
     gene            complement(21545..23308)
                     /gene="argS"
                     /locus_tag="PKN25_01250"
     CDS             complement(21545..23308)
                     /gene="argS"
                     /locus_tag="PKN25_01250"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNV89550.1"
                     /translation="MSADPKVLLTDLLQTALKSVAPELAATPILLERPKQASHGDFAT
                     NLALQLAKPLKRNPRELAGLLLAELPPSKLVARAEVAGAGFINFTLASDAKTAVVREV
                     LAKGADFGRGAGKGIKVQVEFVSANPTGPLHVGHGRGAAYGASLSDVLAFAGYEVSRE
                     YYVNDAGRQMDILALSTWLRYLAFFGIEIPFPPNAYQGDYVVDMGREMRDAHQDRFAK
                     VTAMQILAGTPGLPPSDRKDDEAKQQREEHLDALIANAKKLLGEDYPWVHGFALNEQL
                     GDGREDLQEFGVRFDKWFSEQSLFDTGLVERAVIQLEKAGHIYLQDGAKWFRSTAFGD
                     EKDRVVQRENGLFTYFASDIAYHLNKYERGFDRMIDIWGADHHGYIPRVKGAIAALGL
                     DPARLDVALVQFAVLYRDGQKTSMSTRSGEFVTLRELRREVGNDACRFFYVLRKSDQH
                     LDFDLDLAKSQSNENPVYYVQYAHARVCSVLDQWGGEAAELAGADLGLLQNERELALC
                     ARLASFAELVQDAAAGYAPHQIAFYLKDLAADFHSWYNAERMLVEDEALKLARLALAA
                     AVRQVLVTGLKMLGVSAPESM"
     gene            23480..25009
                     /gene="ubiB"
                     /locus_tag="PKN25_01255"
     CDS             23480..25009
                     /gene="ubiB"
                     /locus_tag="PKN25_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HNV89551.1"
                     /translation="MRLFRLAKIVSVSLRFGLDRMVLDADSSGRLAHVWHKVFFWRTF
                     SESRGARLRQALESLGPIFVKFGQMLSTRRDLLPPDLADELALLQDRVPPFPTEQALE
                     VLEGFYGRPIDEVFDDFERTPVASASVAQVHFARLPDGTDVAVKVLRPGIERVIEHDL
                     ALLEVAAVLLEKIWPEGRRLKPREVVAEFSKYLHDELDLMREAANCSQLRRNFTDSSL
                     LIVPEVYWDWCGSKVMVMERMRGVPISQTPALLAQGTDLKALSRAGVEIFFTQVFRDG
                     FFHADMHPGNIFVHQDGRYIALDFGIMGTLNEVDKNYLATNFLAFFKRDYRRVALAHI
                     EAGWVPAKTRVDEFEAAIRTVCEPIFDKPLKDISFGKTLLRLFQTARRFEMEVQPQLV
                     LLQKTLLNIEGLGRQLDPELDLWKTAKPFLERWMDERMGVRALVRGVKEEAPAWAGTL
                     PQLPRLVHQALTESARHQSAQQQRLEELAAGQRTQGRLLLFIGAVAMGLLGLELYRLF
                     G"
     gene            25176..26636
                     /locus_tag="PKN25_01260"
     CDS             25176..26636
                     /locus_tag="PKN25_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004378848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNV89552.1"
                     /translation="MLLWLVVAYLVVSIGIGLYAATRVHNARDYITAGRNLPMIVVLA
                     MVFATWFGAETVLGISATFLEEGFHGLISDPLGASACLVLFGLVFARPLYRMNLLTLG
                     DFFRVRYNRTTELVLSICIVLSYLGWVAAQVTALGLVFNVLSGDAVTMNQGMLIGAGV
                     VLVYTLFGGMWSVAMTTFVQMIVIVIGLVWVSWIAGDMAGGFDNVIAKAAAEGKFEFL
                     PALDAVEIVAWIAAFATMALGSIPQQDVFQRVNSSRNERVAIWGTTLGGVSYFFFAAV
                     PLFLAYTANIIDPGMVERLMEQDSQLILPTLILQYMPFYAQVIFFGALLSVIMSTASG
                     TLLAPSVTFSENVLRGFFPGMGDRAFLLSTRVTVVLFTAFVTWYATSTESSIHEMVEN
                     AYRVTLAGAFVPLAAGLFWKRANNLGAALAIILGLSTWILFELFVPEGDIEPQLYGVA
                     ASALGMLAGGLIGRQPHHRPHIGGHHAAAGTHHTHR"
     gene            26763..27779
                     /locus_tag="PKN25_01265"
     CDS             26763..27779
                     /locus_tag="PKN25_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNV89553.1"
                     /translation="MSVSSPIELVCPAGSLPALKTAIDHGADCVYLGFKDATNARNFS
                     GLNFDPAQVREGVAYAHARKRKVLLALNTYPQTDNWSSWTQAIDRAADYGVDAVILAD
                     PGLMAYAAKTHPQLRLHLSVQGSATSYEAINWYRERFGIQRAVLPRVLSMAQVESVVA
                     NTQVEIEVFGFGGLCVMVEGRCALSAYATGESPNCNGACSPGKHVRWEDTPRGMETRL
                     NGILIDRFSEGERAGYPTLCKGRFEVNDETYYALEEPTSLNTLELLPELARIGIRAIK
                     IEGRQRSPAYVAQVTKVWRAALDKLNSGGAAGFSVLPAWMAELNKVSEGQSHTLGAYY
                     RPWK"
     gene            27897..28793
                     /locus_tag="PKN25_01270"
     CDS             27897..28793
                     /locus_tag="PKN25_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNV89554.1"
                     /translation="MKLALGPLLYYWPHQSVLDFYADLAEAPLDSVYLGETVCSRRHE
                     LRLDDWLEVGKVLAAAGKEVVISSQALIESESDLKTLRRIVRQHDFRVEANDMGAVRL
                     LVEAGVDNWIAGPTLNIFNPTTLQLMIDSGATRWAVAPEMSGAALAEVRAALARPIET
                     EVFAYGRLPLAHSARCFTARHFNLQKDTCEFRCLSIMDGITLRTREGAPFLTLNGVQT
                     QSARVHNLLGDLPAVRASAEVLRISPQGAHTRQIVELFRAALDEQRGPADALAASREW
                     MVEEPCNGFWHGRAGVEQYVAA"
     gene            28830..29366
                     /locus_tag="PKN25_01275"
     CDS             28830..29366
                     /locus_tag="PKN25_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNV89555.1"
                     /translation="MPALPALPRPPLPSHLLPGSLRQRIGERLATLRIPDFTVPAPVA
                     KLVSRLPQQPPTHVLTLALNLALDRILPRDTLEPLSGRHLQMRVLDAGLTLDFTLGER
                     GFRRATPAAGARPDLILSATTRDFMALALREEDPDTLFFSRRLRMEGDTDLGLLVKNT
                     LDAVDWDALKAKLLGRHG"
     gene            29487..30446
                     /gene="thiL"
                     /locus_tag="PKN25_01280"
     CDS             29487..30446
                     /gene="thiL"
                     /locus_tag="PKN25_01280"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNV89556.1"
                     /translation="MPSEFDLIRRHFTRPARHTELAVGDDAALLAPRPGMRLAVSTDM
                     LVAGTHFFADTDPEGLGWKTLAVNVSDLAAMGAEPRWAFLALALPQADEAWIAAFARG
                     FFACADAFAIDLAGGDTTRGPLTLSVTIVGEVPAGAAITRGGARAGDDLWISGQPGRA
                     ALGLAALRGETGLDAIHHAEFVDALQRPQPRVALGLALRSLASAMLDVSDGLLGDLRH
                     LLECSGTGAIVEVGALPLAALAASGADPALCRRCLLAGGDDYELLFAAAPRHRAEIDA
                     LSARLDLPLHRIGEFTPARERLLLREADGGLAAPAACGYDHFA"
     gene            30478..30960
                     /locus_tag="PKN25_01285"
     CDS             30478..30960
                     /locus_tag="PKN25_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNV89557.1"
                     /translation="MRPTARLLMSHPAHFISLGFGAGLSPWAPGTVGTLLGWAIYPLI
                     RTPVSEFVFLALLASFFLAGVLAAERTGQALGVPDHGGIVWDEMVAIWLVLLFTPAGL
                     LWQAIAVALFRFFDIVKPPPVRWADRSFKGGFGVMFDDLVAAGYTLLALAVLVHLLGS
                     "
     gene            30965..31453
                     /gene="pncC"
                     /locus_tag="PKN25_01290"
     CDS             30965..31453
                     /gene="pncC"
                     /locus_tag="PKN25_01290"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HNV89558.1"
                     /translation="MTDHELDALARRVGEALANRRLWLATAESCTGGWIAEAVTAIAG
                     SSGWFDRGFVTYSNDAKADMLGVRAATLETHGAVSEATVGEMAAGALDHSRADLAVAV
                     SGVAGPGGGSPAKPVGTVCLAWARRDGPTGVVTVHLDGDRAAVRRQTVILALQGVLER
                     CV"
     gene            31650..32687
                     /gene="recA"
                     /locus_tag="PKN25_01295"
     CDS             31650..32687
                     /gene="recA"
                     /locus_tag="PKN25_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNV89559.1"
                     /translation="MDENKAKALSAALSQIEKQFGKGSIMRMGDGSIERDIQTVSTGS
                     LGLDIALGLGGLPRGRVVEIYGPESSGKTTLTLQVIAEMQKLGGTAAFIDAEHALDVG
                     YAEKLGVDIEDLLISQPDTGEQALEIADMLVRSGGVDIVVIDSVAALTPKAEIEGEMG
                     DQLPGLQARLMSQALRKLTANIKRTNTLVIFINQIRMKIGVMFGSPETTTGGNALKFY
                     ASVRMDIRRTGTIKKGDEVVGSETRVKVVKNKVSPPFKEAHFDILYGEGISREGEIID
                     LGVEHKIVDKSGAWYAYKGDKIGQGKDNSREFLRANPVLAREIENKVRVAVGLPELPP
                     AAASAPAPAEA"
     gene            32704..33153
                     /gene="recX"
                     /locus_tag="PKN25_01300"
     CDS             32704..33153
                     /gene="recX"
                     /locus_tag="PKN25_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HNV89560.1"
                     /translation="MGEPSLRERALRHLARRDHSRAELARKLAPHGEPAEIEAVLERM
                     RELGLQSDERYAEAFVRGKAARFGTARLRNELVRRGIDRDRIDEALADECVESEIERA
                     RAVLHGRFATPPADAREWARQARFLQTRGFAPELIRKLLKEPYDESA"
     gene            33140..33862
                     /gene="lptB"
                     /locus_tag="PKN25_01305"
     CDS             33140..33862
                     /gene="lptB"
                     /locus_tag="PKN25_01305"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNV89561.1"
                     /translation="MSLLKVAGLQKRYKARTVVHDVGFEVGSGEVVGLLGPNGAGKTT
                     CFYMIVGLVRADGGQIALDGTDLTHLPIHARARLGLSYLPQEMSVFRKLSVAENIRAV
                     LELQGLERRQIEERLEELLMELGIHHLRDNTAISLSGGERRRCEIARALATDPRLILL
                     DEPFAGVDPIAVIDIQKIIRFLKERGIGVLITDHNVRETLGICDRAYIISEGRVLASG
                     EPTEIIENERVRQVYLGEHFRL"
     gene            33885..35339
                     /locus_tag="PKN25_01310"
     CDS             33885..35339
                     /locus_tag="PKN25_01310"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNV89562.1"
                     /translation="MKPSLQLKLSQHLTLTPQLQQSIKLLQLSTIELNQEIERVLLEN
                     PMLEREDGDGGVDYAPAPPPGNAPEREAAPEPAAEPAGESITHEGGEGSSEDEGIDWS
                     NVGQGASSRSEDDEDGDYQDIQAAGVSLREHLDQQVALSPLSDRDRALVRFLIEALDD
                     DGYLHQPLEDLLELLPQDAEVDLDELAIALRHVQSLEPAGIGARSPQECLALQLQALP
                     ECPIRPLALEIVTSHLELLAERNFARIRKLTGCDDEGLREAQALICSLDPHPGSRYST
                     AETRYVLPDVVVRKLRGQWTVSLNQEAMPRLRINRLYASLLQQNRAQGGGLGGQLQEA
                     RWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGDVAMRPLTLREIADQLELHESTVSRV
                     TTQKYMATPRGVFELKYFFGSHVATDTGGAASSTAIRALIRQLVDAEDSKKPLSDAKI
                     AEILGQQGIVVARRTIAKYRESLNIPPVSLRKSL"
     gene            35358..35681
                     /gene="raiA"
                     /locus_tag="PKN25_01315"
     CDS             35358..35681
                     /gene="raiA"
                     /locus_tag="PKN25_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNV89563.1"
                     /translation="MNLNITGRHVEVTPAIREYVTSKLDRVIRHFDNVTSVTVILSVE
                     KLKQKAEVTLHVRGKDLFVEADDADLYAAIDAMTDKLDRQVQKYKQKQSDHNHESLKH
                     QVPEA"
     gene            35818..36303
                     /gene="ptsN"
                     /locus_tag="PKN25_01320"
     CDS             35818..36303
                     /gene="ptsN"
                     /locus_tag="PKN25_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HNV89564.1"
                     /translation="MSLISQLLPLSNVVIDLDASSKKRVFEQAGLLFENNQGIARSTV
                     FDSLFTRERLGSTGLGQGIAIPHGRIKGLKDAAGAFLRLATPVQFDAPDGRPVDMLFV
                     LLVPEQANETHLQLLSELAQMFSDREFRETLHGATDAAQIHRLFVNWGADAANQPDPA
                     V"
     gene            36272..37219
                     /gene="hprK"
                     /locus_tag="PKN25_01325"
     CDS             36272..37219
                     /gene="hprK"
                     /locus_tag="PKN25_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNV89565.1"
                     /translation="MRQTSLTRLYEAHRESLSLVHVSGPIGARLSVAEERIWPADLVG
                     HLNLIHPTRLQVLGAAELAWAQRQANEKVAHHLNEILSAHPPAIIVADGCEIPNILHG
                     ICEAHDVALFTARHPSASVIDQLRLYLSRELAEKVSLHGVFMDVLGLGVMITGDSGAG
                     KSELGLELISRGHGLVADDVVELSRIAPTVLEGRCPEMLQDFIEVRGLGLINIRTIFG
                     ETACRRKMRLRLICHLDRRQPGPDDPCRLPLQQETQDILGVKVARVVLPVAAGRNLAV
                     LLEAAVRSTILKLRGIDSTQEFIDRQARALHAEEAYRAD"
     gene            37292..38815
                     /gene="ilvA"
                     /locus_tag="PKN25_01330"
     CDS             37292..38815
                     /gene="ilvA"
                     /locus_tag="PKN25_01330"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HNV89566.1"
                     /translation="MSSTPDYLERILNAQVYDVAVETPLDLATNLSARTHNRIYFKRE
                     DMQPVFSFKIRGAYNKMANLSPQALKRGVICASAGNHAQGVALSAARLGVRAVIVMPS
                     TTPQIKIDAVKARGGEVVLAGESYSDAYTHALELEKSEKLTFVHPFDDPDVIAGQGTV
                     GLEILRAHPKPIHAVFCCVGGGGLIAGVAAYIKRLRPETKIIGVEAVDADAMTRSLAA
                     GKRIALEQVGIFADGAAVKEVGAETFRLCQQYVDEMIVVDNDAICAAIKDVFEDTRSI
                     LEPAGALAVAGAKEYARRNKLRDKSLVAVASGANMNFDRLRFVAERAELGEQREAVLA
                     VTIPEKPGSFRKFISVLGNRNITEFNYRYADAERAHIFVGVTVHNRSEVGRLVEMLER
                     HELPALDLTDDEMAKSHVRYMVGGRAPQVENEVVYRFVFPERPGALMNFLSNLRSDWN
                     ISLFHYRNHGSDFGRVLVGMQVPPADKDAFDAFLQRLGYDYVAETDNPAYRMFLSGS"
     gene            complement(38853..39443)
                     /locus_tag="PKN25_01335"
     CDS             complement(38853..39443)
                     /locus_tag="PKN25_01335"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNV89567.1"
                     /translation="MSFAPLSHDPGARRQRMREHALALRERLPAERRERLAAAIGEHL
                     DDLVGRLQPRRLAFCWPYRAEPDLRPWVQAWRAAAPGRVAALPVVLERHAPMMFRAWT
                     PGVPMPLDRHGIPHPADGEAVEPELVLIPLNAFDERGYRLGYGGGYFDRTLAVLRTVA
                     VGVGFEVGRVPDTLPQEFDRPMDWLVTEAGVAKPRA"
     gene            39489..41432
                     /locus_tag="PKN25_01340"
     CDS             39489..41432
                     /locus_tag="PKN25_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNV89568.1"
                     /translation="MKKALVAALTAATFLAPAGAFAQIGGESGDGRIVAAREALRKGD
                     RSTLERLAAVREAHPLDAYPRYWLLINRLARAEEPVPVAALQAFLAEEAGSNLAERLR
                     ADWLRRLARDGDWNGFLAVYADLRSPDAELRCNAWSARVLTGERSVFAELAREWDTLA
                     DADPTCDTPLRAAVDSGAVDEERVWWRIRRQIDTRKPEAALASLSLLPAGNAPAHADL
                     AQAIRSPAPWLDRLQPNFAVSRAGRELALAALVRLAREDVSAARLRLLRIQDRLGAAE
                     RNYAHLALGMHAALDRLPEASALYAAAGDIETTAQQRAWRVRAALRIGDWHAVREAIE
                     AMPADEREAADWIYWLGRAHAAAGRRDAAESLYVRIAGEPHFYGMLAREELGEAFPPP
                     PATAPLPRAKLEEAERDPGLQRALALYRLELRTEALREWVWGVRERDEQFRLAAAHLA
                     LRNELYDRAINTAELANPRSNFELRFLTPYRDLIEPQVRAQGLDLGWVYGLMRQESRF
                     VVPARSSVGAQGLMQVMPATGKWVADRIGLAGYNQRLLTDPETNVLLGTSYMRLILEG
                     LDAHPVLASAGYNAGPGRARRWRDAAPLEGAIYTETIPFDETRDYVKKVLANAVIYAA
                     MLEKRPQSLKARLGTIAPGAASE"
     gene            41489..42445
                     /locus_tag="PKN25_01345"
     CDS             41489..42445
                     /locus_tag="PKN25_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HNV89569.1"
                     /translation="MNPKRVVIVGGSGFIGSAIANRLCKEGVKVLIPTRRRSRAGHVL
                     LLPNVEVVEGNVHDVALLARLFEGADAVVNTVGVLHSRPGRPFGPDFARAHVELPQKI
                     VAACRTAGVPRLVHISALGAYSDGPSEYQRSKAAGETAIRTGSPEIAWTILQPSVVFG
                     RGDSFLNLFADLARMFPVLPLAGATARFQPVYVEDVAEAVWQSLTRDDAGGQTFQLAG
                     PTVYTLRQLVEYVSALVGKPRPIYPLSEKLAMWQARLMELAPQPLMSRDNVRSMKVDN
                     VASGTPQPFGMVPTALETVAPAWLGQAAPRARYYPFRRHARR"
     gene            42471..43115
                     /locus_tag="PKN25_01350"
     CDS             42471..43115
                     /locus_tag="PKN25_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNV89570.1"
                     /translation="MKLIIGNKNYSSWSLRAWLAAREGGFTFEEIHISLFIPGSRERI
                     LSHSPSGKVPCLNDHGVVVWDSLAIGEYLAEKRPGLLPADPVARALARSVTAEMHSGF
                     QNLRSRMPMNVRKDYTGFGHSPEVDADIARIVAIWNDCRARFGAVGPYLFGRFSLADA
                     AFAPVAFRFQTYAVKPAGAAGEYLATLLANPHMQEWATAARAETESIPEEDLYG"
     gene            43115..44341
                     /locus_tag="PKN25_01355"
     CDS             43115..44341
                     /locus_tag="PKN25_01355"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HNV89571.1"
                     /translation="MQAYVVGGAVRDALLGLPVKDRDWVVVGSTPEEMLARGFRPVGR
                     DFPVFLHPHTNEEYALARTERKSGHGYTGFVCHASPEVTLEEDLLRRDLTINAIARAE
                     DGRLIDPCGGRRDLAARVFRHVSPAFAEDPLRILRVARFSARFTDFELAPETLELMRA
                     MVVAGEVDHLVAERVWQELARGLMEARPSRMIRVLRACGALARLLPELDRLFGIPQPE
                     KYHPEIDTGEHVLMVLDHAAAADEPLAVRWACLLHDLGKGDTPAHLLPHHYGHEAASA
                     RRAREVSERLKAPADCRDLAEMVAREHGILARAHELRAETMVKLIERCDGLRRPQRFL
                     LMLQAAACDHAGRGSGAENASEWRPLSRWQAALQAVRAVDAGRIATECSDKSLIPQAL
                     HTARVAAVKALDKDPA"
     gene            44338..45141
                     /locus_tag="PKN25_01360"
     CDS             44338..45141
                     /locus_tag="PKN25_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV89572.1"
                     /translation="MNATRFGELEVLCHAPERATHEHPLLFIHGAYVSAWCWDEHFLP
                     WFARRGWQAYAVSLSGHGRSRQREHLDSYSIDDYVRDVAEVAARLPAPPVLVGHSMGG
                     MVVQKYLEQHDAPAAVLMSAVPPQGLMGSAFGLIFKRPHLLSDLNRIMSGNDVDIDSL
                     REALFHQPVAADDLQRYYRLSQPESHRAIWDMSLFNLPQPARMHRVPMQILGAEHDAL
                     IPPDQVHMTAMTYGRQAEILPGMGHGMMLEHDWEIAAMRIADWLAERAL"
     gene            complement(45158..46333)
                     /locus_tag="PKN25_01365"
     CDS             complement(45158..46333)
                     /locus_tag="PKN25_01365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNV89573.1"
                     /translation="MSSLPQPSADALAQSARLLEHIEAELAAAAGWIPFARYMELALY
                     APGLGYYSGGARKFGPGGDFITAPELTPLFGQALAAQVEQVMRASAPALIEVGAGTGL
                     LAADLLLELERRGCLPERYGILELSGELRERQFDTLAAKVPHLAARVQWLDALPERFS
                     GAVVANEVLDVMPVHLLVSRAEGLFERGVAIATDAAGIRRLCWADVPAAGAVAEGARA
                     LALPVPQSGEYVTELNLAGKAWVAAWAERLHAGALLLIDYGYPRAEYYLPSRSGGTLL
                     CYYRHHAHGDPFLWPGLNDITAFVDFTAVAEAGFEAGLDVQGYTTQAQFLFNCGVLEC
                     LERRGARESADYIRAARAVQRLTAPQEMGELFKVIALSRAIDGPLLGFARGDRTHAL"
     gene            46425..47192
                     /locus_tag="PKN25_01370"
     CDS             46425..47192
                     /locus_tag="PKN25_01370"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HNV89574.1"
                     /translation="MRTSPPEPESTAAPLILVTGAARRVGAAIARRLHAGGARLALHY
                     RHSAGEAEALAAELCALRADSAFTVGGDLARFGAAAEIAAAVLARGNRLDGLVNNASS
                     FFPTPLGSVDRAAWDELMGSNLMGPLFLTQALAPALREARGAIVNIVDIHAQRPLAGY
                     PVYCAAKAGLAALTRSFAIELAPAVRVNGVSPGPIEWPEDDQFPPAERERIVAHTLLG
                     RVGSPDDIARTVAFLMFDAPYITGQILAVDGGRSAHL"
     gene            47259..48212
                     /gene="ttcA"
                     /locus_tag="PKN25_01375"
     CDS             47259..48212
                     /gene="ttcA"
                     /locus_tag="PKN25_01375"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004384785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNV89575.1"
                     /translation="MTAASAPAAALPTTEAGGDARFSNTFLRLKKKLERGVGEAIGDY
                     NMIGDGDTVMVCVSGGKDSYTLLSCLLALRERAPVDFRIVAMNLDQKQPGFPDHVLPA
                     YFESIGVEYRIVTEDTYSIVKDKIPEGKTTCSLCSRLRRGIIYRTATEIGATRIALGH
                     HRDDMLETLFLNMFFGGKIKAMPPKLVSDDGKHVVIRPLAYCTEADIARFARTMDFPI
                     IPCNLCGSQENAQRKLIKNMLQGWARDYPGRIESLATSLKNVVPSHLSDARLFDFVGL
                     TQHTVVEEGDTAFDPPELPDRTLGASVKLFRAGDQEERECA"
     gene            48338..49210
                     /locus_tag="PKN25_01380"
     CDS             48338..49210
                     /locus_tag="PKN25_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNV89576.1"
                     /translation="MPERKNLCVLVALHPVDAVLAERLGADEARHAAERHARRIERAI
                     VMQGGQVLLGGAEQTIAGFERSDAAVHAASEALDRIHGLPPLLGSKQTVRIGVHYGVL
                     DNDGEEPAGEGPAVARQLAHLAESGQALISGTAVMVLGGAARQQVGSQPLSGPVWTRV
                     GIPVHAIGQRSGLVTSVPGAARLSSRLRLRHQQEVLFVEELRPVLLLGRELANDVTIM
                     DPRASRQHARIERRPEGFVLFDQSTNGSFVAIEGKPECFVRQEQMLLDGPGRLGCGFS
                     TTELERDLVFFDIV"
     gene            complement(49290..49793)
                     /locus_tag="PKN25_01385"
     CDS             complement(49290..49793)
                     /locus_tag="PKN25_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HNV89577.1"
                     /translation="MKRILGATGVVLVLAALTPAQAWANPQHERMKRCNAEAKTQALG
                     GDARKAFMSTCLSGKHEAAAASAPGAAPALASGSAAGVPAAAPVAAPAMVPAAAPAVA
                     PAAAATAASAAGAAAPALAVAPTAGSAQKVSSEDMARQKACNRAATEQSLRGAKRKAF
                     IGECMSG"
     gene            49920..50333
                     /locus_tag="PKN25_01390"
     CDS             49920..50333
                     /locus_tag="PKN25_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNV89578.1"
                     /translation="MLNEDSTLLAGLAASAFLAATLLPGGSEAVFAALLALRPELTPA
                     ALLVATAANTAGGMSTYALGRLLPRKESGAHARTLLRWGSLALLLSWVPLIGDALCAA
                     AGALRLNAFACLAWMALGKGLRYAAIAWALPSAGA"
     gene            50413..50763
                     /locus_tag="PKN25_01395"
     CDS             50413..50763
                     /locus_tag="PKN25_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNV89579.1"
                     /translation="MSAIERHGVTARYADAVIHAGVLHLVEVPASSAGSIEAQTREVL
                     ESVGRQLAKLGSDPARIVMATIYLVDMADYAGMNAVWDAWVPPGSAPARACVQVAGLA
                     DPGWRIEIALTAAR"
     gene            50888..>53186
                     /locus_tag="PKN25_01400"
     CDS             50888..>53186
                     /locus_tag="PKN25_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HNV89580.1"
                     /translation="MSQRLREIPYNYTSFSDREIVIRLLGAEAWAVLDQLRTERVTGR
                     SARMLYEVLGDIWVVRRNPYLEDDLLTSRERRDALIGALDHRLNEIDKRRQGNDHVTL
                     LVARAREAVSNFERWFDDTARRRKAVLKALARHTRRDNICFDGHARVSHVTDATDWRV
                     EYPFVVLYPDTEEEMAPLVRECIALGLTIIPRGGGTGYTGGAVPLDASSVVINTEKLL
                     AMSAVEPTVLPGIDGAMAEPYPTIRTGAGVVTERVSEAAALAGRVFAVDPTSASASCI
                     GGNIAMNAGGKKAVLWGTALDNLAWWKMVTPDGDWLEVERLDHNFGKIHEQETVHFRL
                     KRFNPKGDKLLSEEILSLPGASCRKVGLGKDVTDKFLGGVPGVQKEGTDGLIVAARWV
                     LHKMPPVTRTVCLEFFGQVREAVPAIVEITDFFKPGGAGHAAGVQLAGLEHLDERYVK
                     AVGYATKARRHGRPKMVLIGDIVGHDEDAVMKAASEVVRMTNARGSEGFIAVSPEQRK
                     RFWLERSRTAAISRHTNAFKINEDVVIPLPRMGEYCDGIERINIELSLQNKIALCDRL
                     AEFLQGELPLEIKDEGLDREALVSDHRAQALEQVAGVRRRWQWLLDKLDLALAEAEPQ
                     FAAYGVQAGELSNRAASPTLFHRLQDYSIRVSWKEELRPLLEQTFDGALFAPILARIA
                     EIHREVLRGRVFVALHMHAGDGNVHTNLPVNSDHYEMLQTANRAVDRIMALARSLDGV
                     ISGEHGIGITKLDYLTDDEMANFWSY"
BASE COUNT         8350 a        19251 c        17428 g         8157 t
ORIGIN      
        1 cctcgaacac cccgttgaag cctgccggga tcacgcacgc ctcgcccggc ccgaactcgc
       61 gtgccagacc gccctcgtcg gtgatgcgga tgcgtccaga gagcacatgg aagaactcgt
      121 cggtgtcgtc ggcgaaggcg atccgccacg cgcccggctc gcagccccac agcccggcgg
      181 acatgccgtc gcgctcgaag tgggcccacg tggcgcgctg cggattgccc gcgaccaggc
      241 gctcgaggcg agggaagtcg aagctcggtt gcgtctttgc ggcggaaaag gcggtgagcg
      301 tcttgtgcat ggcggcagcg gggaggcagt ggaggccgca ttctagcctc gctgcccgac
      361 ctggcgagcc agccccgacc agtcagctcg ccaacgcacg ctccagccca aggcgcaccg
      421 ccgtcctcct ccatgcccga ccgctcgcgc ggccccctgt cctcacgccc ccaccacgtt
      481 cacccggccg ggaggccgcc gcggactgcg cgccggcggg tctagaatga gcgtatcagg
      541 accccaggcc cccacaatga acgcgccctt cctcgccttc ctgacctggc cgcaacgctg
      601 gctgctgttg accgtgctgc tcgccctttc tgcgctcttg ttgttcggac gcatcgcaag
      661 cttggacgag ttcatcaagg ccaggggcga ggggggcatc atggcgctcc agttcgctgg
      721 atcggcggac cgcgccgcgc atctcctcgt cgcctggcgc ggcctggagg atctcttgcg
      781 ctcgcagacc cgctgggact tcctcttcct gctggtctac ccggcggtac tctcgctcgc
      841 ctgcgcgatg ctggccgagg cgcgaggcaa ccctctgccg acgatcggga tcttcgtcgc
      901 ctggctcgca cttgcggcga taccgctcga cgcgatcgag aatctggcca cgctgcgcat
      961 gatcgaccac ggtgcgagcg aaccgctggc gcgatggatc accgtctgcg caacgatgaa
     1021 attcgccatc gtcctaactg ccgccggcta cctgctcgtg gcgggaatcg cgacactctt
     1081 ccagcgctga tgacccggcc gggatcccgc accccccgcg gcgggacggg tgcaggtcga
     1141 caccacagca ccacgccgca cgcccggatc agcgactcgg ggcgccccgg tccgcgcaca
     1201 cccctgcagc ccttacaccg gttccggcat gctgtgccac cagatggcac agcgcgtgca
     1261 cacgccactg ccgaactccg ccttcaacac gccgtcgagc accatgcgcg gcagcgggtc
     1321 gcccggcttg cgcgcgacca ggttggtgcc ggtggattgc gcccagatcc ggaacaactc
     1381 ctccgaggcc acctggtagg tgacgtgcca gtgcgcgatg ccgccggcgg gcgaggtggc
     1441 gaggatctcg tagcgcacct gcacgtcctc ctgcagcttc acgcgctgcc agtaggcggc
     1501 gagttgcgca tgtccgcgct gcaccgcaaa gggggtgttg tggtattcgc catcctccgc
     1561 gaacagggcg cagaactgcg cctcgtcgcg ttcctcccag gccttcttgt acccttgcat
     1621 gaattgttcg atatccatct ttcagcctcc tcgctctctt gttatcgtcc acctcccagc
     1681 cacggcagta taggcgccac ggcgcgcggg ccacgaaggc ggaacggatc agagggcgga
     1741 cgacatgctg ggaaacagcg aacgggaacg ggtgcacacg cgccgcatcg tgtgcgaggc
     1801 ctttcggcgc cgcgacgggc tgtgggaaat cgaggcgacg gtgatcgacg agaagggcga
     1861 ggacatgccc ttccgctctc gtccgatggt gcgcccgggg caatccctcc acgacatcac
     1921 gatcgccgtg ctgatcgacg acgacgccgt gatccacgac gtcgccgcgc ggatgcagac
     1981 cgtgccctgg gcggcctgtc cggaaagcca ggccgcctac cgccgcctga tcgggctgca
     2041 gatcggcgcg ggcttcatgc gcgaggtgcg tgagcgcgtg ggcggggtgg agggctgcac
     2101 ccacctcacc gacctgctcg tgcaggtcgg caacacctac acccaggcga tctggccgac
     2161 gctgatcgcc cgccagttcg ccgccgagcc ggacccacga aaatggaccg accgccgcgc
     2221 ggtcggcttc gtcggcggct gcgcggcgtg gcggcaagac ggggacacac tcaggcagga
     2281 atatccggag ctggcggcgg agttcgtcgc gaagcgggac gaaacctgag gccagaaccc
     2341 ggcaacgccc acccgccggg cgcaggtcac accttcagat cgacgacctg cccgggctgg
     2401 acgctcagcg caggccggac accggccgcg ggggtgtaca ggtcagagga tgctgtgggc
     2461 gcggggatcc ggccggattc ctgcagccca agcccctggg aagggcccgc ctcggccgca
     2521 tcggcgagcc ggccgaccgc cgccgataag gtctgcgcca cacggtccat gccacgcacc
     2581 gaggctaccg cttgcttgcc gatttccgcg cgggtgcgtg cctgccccgc ctgggtctga
     2641 agttcgtcgg cgcgacgttg ggcagtgtcc gcctcgtcac gcgcgctcgc agcctccgcg
     2701 gagagcgcgg ccgccttggc ctcgagctgt tcggcacttc gccgtgcctg ctcgcgcgag
     2761 agctgcgcgc cgacccagga ggccgtggag gcggaggatc cgagaccttg tatggacatg
     2821 atggccggcc ctcagcgggc ttgagcaccg ctgcggggac tcaggtacgc aacacgcccg
     2881 gctcgcgctg gcccggcgcc gtcggttgct caggctggct cgggcgcgcg gcagcattgt
     2941 tggcgcccac ctgcgacatc gcctcgtagg acgcgagcgc acgttgcgcg gagtagttgc
     3001 tccgcaggtc gccgttggct gccgcagcac gcgcctgctc gatcgcacgt gcctcgaccg
     3061 agccgccctg cgcaggcggc tgcaccacgc cctcgccacg cgcggccgcg ctcagcgtga
     3121 ccaccgcgct cggctgcgcg acctggcgcg cagaagggtc gcgcgctgcg tcctgcgcac
     3181 gcgcgccctg ctgctcgcgc tcggcgctcg cgacctgacg cggatcggtc ggtcgcatga
     3241 cttcgcggga gaccggattc attccagagg acacggcttc catgacactc gcctcgcaga
     3301 tcgttcttca atcattccag cgcaccggag ccgccacccg cgggcggccg ctgcgattca
     3361 tccagtgccc ttgtcgcgga tgttctcacg aaatcagcct cgctgcgtgt tgcacggatc
     3421 acatccgcca ggtggacgct cggctggatg gctggactgc caccgaacgc cgcagacccg
     3481 aacggcgcga ctcaccagaa aggacggtgc tgtcgggatc cctttcttcg ccttcacttt
     3541 gccttgctac cggaaggcgc cacgtcttta atgacacgca tcaacccgct gtccgtgtgg
     3601 aaatcgcggc gaggcccgtt gaacgttgct gattcaccca caagccccaa ggagagatcg
     3661 atgaatcgaa actggacagg tttcaaagtg ctggcacccg ctgcgcttgc cttggtatcg
     3721 caggccgcgg cagcggatgt gctgctgacg ttaggcgagc aagacttcac caacggcagc
     3781 acgcctggta cggccgattt cctgacggcg gggggcaacg aatcggcacc gttcgacggc
     3841 gtgttcagcg gcgccgacac cgcgagcaac ttctcgacaa gctggacttt cgcgtcctac
     3901 ggatcgcagc ccaccatcct cggcgcgagt ctgacgctcg gcatctacga ccatgagtcc
     3961 gccgcgccgg ggaatcaggt cgcagccttc acgctgaatg gcttcgacct gaccagcgcg
     4021 atgagcacgc tgttcgaagc cagtggcggg gcgcgatcgg aatacgacgt ctatacgctt
     4081 acgctaccga gttccgtttt cgccacgctc ggaaccggga cggccacctt cgcccttgcg
     4141 ctgcagggcc cgggcctggg cctgttcggt gagaccgcgt tcaacggcgc agcgctcgat
     4201 ttctcgaccc tgaggatcac gaccaccgca cagccaccga cgaacaacgt gccagagccg
     4261 gcaaccggtg ccttgctgtc gatggcattg gcaggtttcg ccgccacgcg ccgcaggcga
     4321 gcgcagtagg ggcggcccac cgaccgcggc gggcagattc aggcggcccg cccccccggg
     4381 gcaggccgcc attccatttc cagatagacc cctttgtcac ggagcacacg gaaccccagc
     4441 cgcaggtaaa gagattgcgc gggactctcg cgctcgacat gcaggctaac cgcgcggccg
     4501 gccacgcggg cttcctccag caactcaccc agcaggcggc tgccaatgcc gcgcccctgc
     4561 cacgcccgca gcagcgccac gtcgatcacg tgcatgctcg gcgccgaccg gtcgacgtac
     4621 aagcgaccga tggacgcggc accccactcg acgatactga acgatgcagc tggaaaactg
     4681 cgccgatatt gcgttgtctg caacgtgaat tgctggcgga gcagcgcatc ccgttgagcc
     4741 ggctcgagag ggatattcgc gaaaccgggc gcacgcgctt cgacaaaaag cgtgaagacg
     4801 aaggcttcgt cgttcccgca ttcggcccgc agcgccaccc cccggcttgc gcggagcgca
     4861 gcggcggaca gtggtctcgc ttcgccgtcc atcccggcta gtcgcgggac gggaagatgc
     4921 cctgcagcgc gatgcagtag ttcacggcga gatagggctg catattcgcg tgtgcctggc
     4981 ccgcccccgc ggggccgatc gccccggcgg ccagtggtgc atttcccgca ctgctgaaga
     5041 tcacattccc gctggagtcc tgggcccaga cctttccggc cgggctcgac tgggtgcccg
     5101 gcgccgcgct ggccatggct gcatggctgt gctggggcac ttgggcgaca gtgaggctga
     5161 cggtctcggt gccggccgat tgcccctgcg gatgggcttg agcgatgtgc atcgcggctc
     5221 tgcctcgcag atccggcagc gcgaaggtgg ttcgcccgtc gccaccgtac atcgtgccca
     5281 gcagcgcaaa cagcgcctga ttctggttga tcggcagcaa ttgaccgtcg caggtcgccc
     5341 aacccctggg cgcatagcca aagccgaagg gccggatctc gccgacgaaa ggttcactca
     5401 tgaacttgcc tcctcactga tcaccaagcc gaaccgcggc tcctgtgtgt gttgaatgct
     5461 tggcgggcca agccgcccat cgggcgcacg caggcagatc ttgctagccc ctcggcggat
     5521 aaatcccttg cagtgcaatg cagaagttga gcacctggaa gggctggacg ttggagtgag
     5581 gctgcccctc cccggcaatc ccgatcgccc cgggcgccat cgccaccgtg tcagcgtcag
     5641 cgtagtcctt gcttccaccc aggtcctgcg cccaaaagtt gccgcccggg ccgggttgat
     5701 tgccgttcgc cggattggca ttggccgcgt ggccgtggac gggcatctcg cccagcgtca
     5761 gggtcacgga ctccgaacca ccgcgctcgc cttggctacg cggcgtgaga ccactgccct
     5821 ggccactgcc catcgcgacc gctcccgaca gattgggcag cgcaaaattc gtccttccgt
     5881 cgccaccgta catggtaccg agtatggaaa acagcgcggt gttctgcgcg atgggaagca
     5941 gttgcccgtt gcattgggcc caaccgcgcg gcgcgaagtt gaagccgaac gatcggattt
     6001 ctccgatgta gggatcgctc atcgacatct ctcctttcgg atgcgcttca gggtactgga
     6061 cggattgtgc tcagctttgc gacgggaaga tgccttccag cgcgatgatg tagttgagca
     6121 cgaggaaggg ctgcatgttg gcgtgggcct gtccgccgcc ggcagcgccg atcgcgccgg
     6181 tcgccatctg gctgccggcc gttgtgctgt acgcaccggt gttgccccct ggatcggtgg
     6241 accagtaggc ggcggccggc ccgattctgt ccgcacccga gttggcgcca atcgcaacgt
     6301 ggctgtgact ggggagttgc gggacgccga gggcgacggt ttcgctgccg cccgcctccc
     6361 ctagcgcccg cggggtcagg ccgggccccg caccctgatg gatggggatg cggcccgcga
     6421 ggttgggcag cgcaaaggtg gtttgcccgt caccgccata ggtcgtcccg atcagcgcga
     6481 acaaggcgtc gttctgggcg atgggcagaa gctgcccctg acacagcgcc cacccgaggg
     6541 gagcgaaatt gccggcgaag gcccggatct cgcccagaaa tggatcagcc atgacaacct
     6601 ccgtatgccg cgccccgagg gttggcgcct cacggaagag taggcaagga aaccgacgct
     6661 gtcactgccg aaatgagaat ttttgcggaa tggcgacttg cccccggttt ccccgcaatg
     6721 cagcctgctt cgcaacctcg acaacctgaa ctcgagcacc gcctgcagcc tgtcgccgag
     6781 gcgcacgtcg agccgatgcg cgcctcggcc tgggcaacgc gctgcgcgaa ggagtcgggt
     6841 gcggcggacg tgctcggcct tgagccggct gcacatcagg ccgatgaccg tgcgcgtatt
     6901 gccggtgccg gtgaccatgg cgaggagtca gcgcaaacgg ggcaaggccg gcctcccctc
     6961 gcaggaaagg ccggcctcgc tgcgcccgcg gcaaatggcg gccgagggtc agcccgccag
     7021 cgcgcgcccg atcaccagct tctggatgtc gctggcgcct tcgtagatct ggcacacgcg
     7081 cacgtcgcgg tagatgcgct cgaccgggaa gtcggtgacg tagccatagc cgccgtgcac
     7141 ctggatggcg tccgagcaca cgcgctcggc catctcggag gcgaacagct tggccatcga
     7201 ggcctccttg aggcaggggc ggcccgcgtc cttcaggctg gcggcgtgcc acacgagctg
     7261 gcgcgcagcc tccagttgcg tggccatgtc ggcgaggcgg aagttgaccg cctggtgctc
     7321 gaagatcggc ttgccgaagc tctcgcgctc ctgggcgtac ttgaccgcag cctccagcgc
     7381 cgcacgcgcc atgccgagac actgcgcggc gatgccgata cggccggcct cgaggttcga
     7441 cagcgcgatc ctgtagccct cgccttcctt gccgaccacc gaatccgcgg ggatgcggca
     7501 atcctcgaag aggatctggg tggtgtcgga ggccttctgc cccatcttct cctcgatgcg
     7561 gcccacctgg tagccgggcg cattggccgg caccaggaag caggtgatgc ccttcttgcc
     7621 ggcggccttg tcggtcaccg cgaacacgat ggcaacatcg gcgtgcttgc cggtggtgat
     7681 gaactgcttg acgccgttca acacccactc gttgccgtcg cggaccgcgc tggtgcggat
     7741 cgccgaagcg tccgagccca catgcggctc ggtgaggcag aagcagccga gcttctcgcc
     7801 gcgcgcgagc ggcttcaacc acgcctcctt ctgcgcgtcg ctgccgaagc ggttgaggat
     7861 gccgcagggc agcgagttct gcacgctgac gatggtcgag gtggcgccgt cgccggcggc
     7921 gatctcttcc agcgtgagca ccaggctcat gtagtccatg ccggcgccgc cccattcctc
     7981 gggcaccacc atgccgagcg cgccgagttc ggcgagctcg ttcagcgcct cgcgcgggaa
     8041 ggtgtggtta cggtcccatt cggcagcgaa gggggccagg cgctcctgcg cgaaggcacg
     8101 catggagtcg cggatgagtt cctgttcctg ggtgagaatc attgcactgc tgtccttgta
     8161 gaaatccttg ctggtgtggc gctgcacgtc gtaggggcgc cccggcgggt cgcccctaac
     8221 gaaacgcgag tcctggcaga gtcgtctcgc gagcggccgc agcgcttgat ccgattcagc
     8281 cgcaggccac cgacgaatcg acctcgatcg ccatcgcggt ggcttcgccg ccgccgatgc
     8341 acaggctggc cacgccgcgc ttgaggccgc gcttgcgcag cgcgccgatc agcgagacca
     8401 ggatgcgcgc gcccgaagcg ccgatcgggt ggccgagcgc gcaggcgccg ccgttcacgt
     8461 tgaccttgtc gtgcggcagc ttcatctcgt gcatcgccgc catggtgacg accgcgaagg
     8521 cctcgttgat ctcccacagg tcgacctgct cggtggtcca gcccgccttg gccagcactt
     8581 tctgcatcgc gcccaccggc gcggtggtaa acagcgccgg ttcctgcgca tgcgtggcgt
     8641 ggccgatgat ggtcgcgacc ggcttcacgc cgagcttttc ggcggtggac ttgcgcatca
     8701 gcaccagcgc ggcggcgccg tccgagatgg agctcgagtt ggccggcgta acggtaccgt
     8761 ccttcttgaa cgccggcttg agcgagggga tcttgtcgat ctgcgccttc agcggctgct
     8821 cgtccttgtc gaccaccacg tcgcccttgc ggctggccac ggtgaccggc accacctccc
     8881 agctgaagtc gccgttgttg atcgcggcct gcgcgcgcgt ggtcgaggcg atcgcgaact
     8941 cgtcctgggc ctggcgggtg aagttgaagt ggccggcgca atcctcggcg aaggtgccca
     9001 tgaggcggcc cttgttctcc ttggagtagc tgtcttccag gccgtcgagg aacatgtggt
     9061 cgaacatctg gccgtggccg aggcgatagc cggcacgcgc cttgggcagc aggtaggggg
     9121 cgttggacat cgactccatg ccaccggcga ccatgacctc gttgctgccg gcgaggatga
     9181 ggtcgtgcgc cagcatggtg gccttcatgc ccgagccgca caccttgttg atcgtggtgc
     9241 agccggccga cagcggcagg ccggcgccga gcgcggcctg gcgggcaggc gcctggccga
     9301 gaccggcggg cagcacgcag cccatgatca cttcctgcac ctgcgcgggc gcgacgccgg
     9361 cacgctcgac cgcggccttg atcgcgatcg agccgagctg cggcgtggtg aggctggaca
     9421 ggtcgccctg gaaaccaccc agcggggtgc gagcgacgga aacgatgacg acgggatcgg
     9481 acatggaact ctcctgttat gggtgttgat cgattcgatt tgcgcggcgc tcagttgccg
     9541 agcgcctcca gcgccgcgac gtagcgcggc atgagatcgt cggaacggct gaggttcatc
     9601 ccgaggtcgc ggatcagacc gtcctgcagg ccgtagatcc agccatgcac ggtgaggcgc
     9661 tggccgcgcc gccaggcgtc ctgcacgacc acggtctgcg ccacgttggc cacctgctcg
     9721 atgacgttga gctcgcacag gcgatcgtgg cgcagttcgg gcgccaggcc atcgacggcc
     9781 ttcagatggc gcacatggac gtcctgcacg tggcgcagcc aaagatcgac gaggccgacg
     9841 cgcgcacgtt cgagcgcggc cttgacgccg ccgcagccgt agtggccaac gaccatgatg
     9901 tgcttcacct gcagtacatc gaccgcgtac tggatcaccg ccagcgcgtt gaggtcggtg
     9961 tgcacgatca cgttggcgat gttgcggtgg acgaagacct cgcccggcag caggccgacg
    10021 atctggttgg ccggcacgcg cgagtcggaa cagccgatcc agaggtactc cggggcctga
    10081 agctgcgcca gcttgccgaa gaagccgggg tcgagctgct gcatctggcg cgaccacgcc
    10141 tggttgtagt cgaagaggtg gaagaggttc tcgttgctga cctcgtcctc cgaccagttc
    10201 tccaggtcca gggtcaggaa gatcgcaccc tcgatcggcg aggggtagca ggcgggcact
    10261 tccttgaagc cgagctcgcg gtagagcttg accgcgatcc gcatcgacgg gatggtgtcg
    10321 agcaggatgc ggcggtagcc gaacagacgc gccgccttga tggcggcgag cgcgagcttg
    10381 cgccccacgc cgccgccacg gaaggccgga tcgacgtaga gccgcttcat ctcgcacacg
    10441 ccctcggaga agcggcggat gcccacgcag ccggccggct tgccatcgac ctcggcgtag
    10501 aacaggcggc cgtcggcggc gccgtaggcc cccggcaggt tggcgatctc gtcggcgaag
    10561 ccctggaagc tcaggtccac gccgagccag gcggcgtact ggcggaagaa gccgcgcacg
    10621 tgctcgagct cgacggtgtc gttggcgccg agggcgcaca gacgggtggg catggcagga
    10681 ctccgggaag gttcggggac ggatctctcg ggggaggggg aagagcaagc cttcaggcgg
    10741 cgcgctcctt cttgccgtgc atggccatga tcgtctgctg catcagcgca cacaaggtcc
    10801 acttgccgtc gcggatggcg aagacctcgg ccttggtgat gatcagcgtg cgccccggct
    10861 tgaccacctc gccgcgcgcc accaggcgct cgccgtcggc gggggcgacg agattgagct
    10921 tgtactcgac ggtgagcacg ctggtgtcgg ccggggtcag ggtgttggcc gcgtagcccg
    10981 cggccgagtc ggcgatcatg ccgaccaccc cgccgtggac gaagccgtgc tgctgggaga
    11041 tctccgcctt gaagggcagg tggatctcgg tgtagccggg ctcgaccacc ggaatctcgg
    11101 cgccgatcag gcgcattgcc tgctgcaggg cgaagctggc gcgcacgcgc tcggcgtagc
    11161 cggggtcgcg gggctggaac tggggcaatt cggggcgcat cttcgtgtct ccgtcgagtg
    11221 tgtaattatg ccagccaata cgttaacgtt aacgtaatcg atcccattcc ggaacggccc
    11281 gacggcgtga gcgccgtccg cgcacgccgg tccgcacgct gaacgtgccc gccccccgta
    11341 gaggagaccc catgagcccc cccgacccca cggtcctcga ataccccttc gacagcctgc
    11401 cctcgcccgg tcactcgatc gagatcgccc ccggcctgcg ctgggtgcgc atgcgcctgc
    11461 ccttcgcgct cgaccacatc aacctgtggc tgctcgacga aggcgaggcg ctcgcggtgg
    11521 tggactgcgg cttcgcccgc gacgagacgc gcgaggcgtg ggacgccgtg ctcgccgcgg
    11581 acggccggcc actgcgccgc gtcgtggtga cccaccacca ccccgaccac atcggcctcg
    11641 ccacctggct ggccgcgcgc gacgacgcgg cgatccacat gacccagggc gagttcttcg
    11701 ccgcccaggc agtgtggcac cagctgccgg gctattgcgc caccgacatg gtcgagcagt
    11761 tccgccgcca cggcctcgac gagatgcgcc gcgacgccct gctgacccgc ggcaacgcct
    11821 accgcatcgg tgcgccgcag gtgcccggcc gctacgagcg catgtttgcc ggcgacgagt
    11881 tgcgcatcgg cgcccatacc tggcgcgtca tcgtcggctt cggccacgca cccgagcacg
    11941 ccagcctgta ctgcgcggag ctcggcgtgc tgatcgcggg cgacatgttg ctgccgcgca
    12001 tctccaccaa cgtcagcgtt tatgctgcaa cgccgcatgg cgacgccctg ggatggtttc
    12061 tccactcatt gcgagaattc tgcaccttgc ccgacgacac gctcgtgcta ccatcacatg
    12121 gcaaaccttt caagggtatc cgggcacgcg tggagcagct cgtcgagcat catcgcgaac
    12181 gttgcgaggt gcttgtggcg gcttgtgcca tgccgcaaag cgccgcagca ttgctgccga
    12241 cgctgtttcc gcgcgagctc gatacccatc aggtcatgtt cgccatgggg gaagcgatcg
    12301 ctcacctcaa ccatctcgag aagaggggag agctctcgag acgcgtcgat gcggacggcc
    12361 tgatccgttt cacccattgt ccgtaacatt gtccgcaaca gaccgccccg ccgcaaaaac
    12421 aggagcccga acaagatggc cgcacccagc aacgagaccc ccgcctgcga cctgcccgac
    12481 ccgcaggaag tcgccaagac ctatgccgaa gtcgcccgcc gcgcctcgca cctgatcagc
    12541 gaccacgtgc accgccaact caagaagggc gtgagcaccc ccgccgacga gctcggcatc
    12601 gcccaggcct tcatggacat gatggccaag ctgctggcca acccgtacaa gctcgcccag
    12661 gcgcagatga atctggtgtg ggactacttc tcgctgtggc agcactcgat gatgcgtgtc
    12721 gccggcctga gcgcctcgcc cgtcgccgcc cccgagaaga gcgacaagcg cttcaaggac
    12781 gaggagtggg agcagcactt cctgttcgac ttcatcaagc agtcctacct gatcgccgcg
    12841 cgccacatcc acgacaccgt cagcggcgtc gacggcctcg acgagcagac gcagaagaag
    12901 gtcaccttct acacccgcca gtatatcgac gcgctgtcgc cgtcgaactt cgcgatgacc
    12961 aaccccgagg tcttccgcga gacggtcaag agccacggcc agaacctgct caagggcctc
    13021 aacaacctgc tgcgcgacgt cgaggacggc ggcggcaacc tgcgcgtcaa gatgaccgac
    13081 acctcggcct tcgagctggg caagaacgtc gccaccacgc cgggcaaggt cgttttccag
    13141 accgagatga tgcagctgct gcagtacacc ccgagcaccg aaaaggtcct caagaagccg
    13201 ctgctgatcg tgccgccctg gatcaacaag ttctacatcc tcgacctgcg cgagaagaac
    13261 tcctacatca agtgggcagt ggaccagggc cacaccgtgt tcgtgatctc ctgggtcaac
    13321 cccgacgagc gcctggcgca gaagagcttc gacgcctacg tcaaggaagg cgtcatcgcc
    13381 gccctcgacg cgatcgagcg ccagaccggc gagaaggaag tgaatgccgc gggctattgc
    13441 ctgggtggga ccctgctggc gaccacgctc gcctacctcg ccgccaagcg ccagaagcgc
    13501 atcctctcgg cgaccttctt caccaccatg accgacttca ccgatccggg cgaactcggc
    13561 gtgttcatcg acgaaggcca ggtgtccagc ctcgagaaga agatgttcga gcgcggctac
    13621 ctggaaggct cggagatggc cggcaccttc aacatgctgc gcgccaacga cctcatctgg
    13681 tccttcgtgg tcaacaacta cctgctcggc aaggatccgt tcccgttcga cctgctgtac
    13741 tggaactcgg actcgacccg catgccggcg gccatgcaca gcttctacct gcgcacgatg
    13801 tacatggaaa accgcctgat cgagccgggc ggcatcgaga tcgacggcac cccgatcgac
    13861 ctcggcaaga tcaaggtgcc gtgctacttc atttccacga tcgaggatca catcgcgccg
    13921 tggaagagca cctacatggg cgcccgtcgc ttcggcggcc cgacccgctt cgtgctcggc
    13981 ggctccggcc atatcgccgg catcgtgaac ccgcccgccg ccaacaagta cggctactgg
    14041 ctcaacccga gcgcgaagct cgccgagacc gccgacgcct ggctggaagg cgctcagcag
    14101 cacccgggct cgtggtggac cgactggcag gcctgggtca ccgcacacga cagcgagcag
    14161 accgaggcgc gcgatccggt gaacggcaag ctcgaggtcc tcgaagacgc cccgggcagc
    14221 ttcgtcaaga tccgcatcga cgccaaggcc gccgcctgag ccttgtcgcg acgagtacgg
    14281 ggaggggaag ccctccccgt ttttcgttca ccacgctgca tcgccgcgcc ggcgacccgg
    14341 ctcacccact gcgaacgtga ccgaaccctc ctcgcaagca cccctgccgc ccaccctcgt
    14401 gctcgacacc aacacggtgc tcgcgctgtg gatgttccac gaccccgcgc tcgaggcatt
    14461 acgcgcgtgg atcgacgcag gatcgtgccg cctggtctcg cgtgcagatg cgctggaaga
    14521 gttgcgccgc gtgctcgcct atgcgcagtt cggtctcgac ccgggccgcc aggatggcct
    14581 gcgcgcacgc tacatcgacc gcatcgcagt gctggcggac gatgccgacg ccggcacgct
    14641 ccccagctgt cgcgaccccg acgaccagaa gttcctcgaa atcgccggcg ccgccggcat
    14701 ggcgacgctg atcacccgcg acaaagccct gttgcgcctg gcgcgccacc gcctcgtacg
    14761 cgagcgcttc gccatcctca ccccggaacg agtgcagcac acgctcgcac agccgagcgc
    14821 aacgccggcc tgagcccccg gctcacgcca gcccgcgccg cagcgccatc agccagcgca
    14881 ggccgcgcgc ggcacggcta ccgtaccacg acaccatctc gccatcgacc agcagcgccg
    14941 ggcgcgcagc caggcgcgcg acctcggcga gatgcgccgc gcggaagcgg tagggctcgg
    15001 tggacagcag cacgcgctcg actccggcga gccagggcgc attccagtcg aaggcgggat
    15061 agcgcgccgc tccggtctcg ccaccttcca ccgctggcag ggtgctccag ccgacctcgg
    15121 cgagcgtggc ggcgatgtag gtgtcgcgcg cgaccgtcat ccagggctcg cgccagatca
    15181 ggtagagcac gcgctcgtcg gcgagcgccg cgcgcaacgc cgcggcctcg tcgagcactg
    15241 cgcgcagttc gtgggcgagc cgggcggcgg ctccttcagc gccgaagacg ccgccgagca
    15301 gcgcgaagag gacgaggttg tcctgcggcg tgttgggatg ggtgacgacg atgtggggca
    15361 cgaaggcggc gagcgcgtcg acggtctcgc ggcggttctc gtccatgttg acgagaacat
    15421 gcgtgggcgc gagctcgcgg atgcgcgcga gattgacgtc cttcgtcccg ccgaccttgg
    15481 gaacggcgcg caaggcctcg cgcgggtgta cgcagaagcc ggtgcgcccg acgaggcgct
    15541 cgcccaggcc gaggtcgaac accagttcgg tcagggaggg caccagggag acgatgcgcg
    15601 cgtccgcgcc ggcgggcgaa tgcagccgcc cggcagcgtc gatccaggtc gggcacccgt
    15661 cctgtgaagc cttggaatgc atggtcgatg ctcggatgaa acgtgcggag ccgcatctta
    15721 tcgcgccaca ccctcccgtc ctcgcaccct gcggggaatt catgcgcaag ccgcgcacct
    15781 cgggaaatcc cgagtgcagg caggaatcgt tttgtgccaa gcttggccat ccttggtggg
    15841 cgctcgtggc cgctttcgcg gccacccggc tcccgccccg atccgcagca cgtgtcctcc
    15901 ctgtcggcgc cccttcggcc agccggcgtt ccaccttcac ggagaaagct cacatgaaaa
    15961 gactgactgc cctggctgcc gccttcgcaa tcgccggctc gctcggcgcc gcccccgcgc
    16021 acgcccagca gaagttcatc acgatcggta ccgggggtgt gacgggcgtc tattacgccg
    16081 cgggcggcgc gatctgccgc ctgatcaaca aggaccgcgc ccagcacggc gtgcgctgct
    16141 cggtcgaaag caccggcggt tcggtgtaca acgtgaatac catcaaggcc ggcgaactcg
    16201 acttcggcgt ctcgcagtcg gacatccagt acaacgcgct caagggcgag gtccagttca
    16261 aggacggcgg cgccttcgca gacctgcgct cggtgttctc gctgcatggc gagccgctga
    16321 ccgtgctggc gcgcaaggaa gccaacatcg gcaagatgga ggacttcaag ggcaagcgct
    16381 tcaacatcgg caacccgggt tcgggccagc gcgcgaccct cgaggtgctt ctgccggcga
    16441 tgggcatgag cttcgccgac ttctcgctcg cctccgagct caagcccgac gagcacggcg
    16501 ccgcgctatg cgacaacaag atcgacggct tcgccttcgt ggtcggccac ccggcggcca
    16561 acatccagga cccgaccacc acctgcggcg ccaagctggt gccgatcacc ggcgcgggcg
    16621 tggacaagat cctcgcccag tacccctact tcgctccggt caccatcccg ggcggcatgt
    16681 atgccaacaa cccggatgcg accaagacct tcggcgtggt ggcgagcatc gtgacctcgg
    16741 ccaaggtgcc ggtggagtcg gtctacatca tggtcaaggc ggtgttcgag aacttcgagg
    16801 acttcaagaa gctgcacccg gccttcgcca acctgcagcc gcaggacatg atcaagaacg
    16861 gcatgtcggc cccgctgcac gagggcgcgg tgaagtacta caaggaaaag ggctggatgt
    16921 aagtccaccg cttgccacat ccctggcgag caagcccgat gtcacgagaa cggcggcggt
    16981 cccgaggccc tcgccgtttt ctttccatcg tccgtcgaat gcggactttc acgacgaggt
    17041 aggccatggc cagcccggaa aaacaagaac acggtttcga gctcaccgac gaacagctcc
    17101 gccagatcgt cgccgaggcc gacacgggtg ggcgcaagcc gaccggatgg ccggcgcgcc
    17161 tcatcgtgct ggtcgcactg gcatggtccc tgatgcagct ctggatcgcc tcgccgctgc
    17221 cgttctcgct cggcatgttc gtcttcaacg acaccgaggc gcgctcgatc cacctcgcac
    17281 tggccgtgtt tctcgccttt ctcgcctacc cggcactgaa gcgctctcca cgcgaccgga
    17341 tcccgctcgg ggactgggta cttgcagcgg tcggggcgtt ctgcatgggc tacctgttcc
    17401 tcttctacag ggaactggcc gcccgccccg gcctgccgac cacgacggac ctcgcggtgg
    17461 ccgtgtgcgg cctgatcctg ctgctcgaag ccgcacgccg cgcactcggc ctgcccatgg
    17521 tcgtgctgtc ggtggtgctc atcggctacg tctttctggg cccctacatg ccggagatga
    17581 tcgcccaccg cggcgcctcg ctggcgaagg ggatgagcca cctgtggctg accaccgagg
    17641 gcgtgttcgg ggtggccctc ggggtatcga ccagcttcat cttccttttt gtgctgttcg
    17701 gctccctgct cgagaccgcg ggcgccggca actacttcat caagtcggcg atctcgctgc
    17761 tcgggcatct gcgcggcggc ccggccaagg ccgccgtggt ggcttccgcg agcacgggat
    17821 tgatctccgg ctcctcgatc gccaacgtcg tcaccaccgg caccttcacg atcccgctga
    17881 tgaaacgcgt ggggtatgcg cccaagaagg cggcggcggt cgaggccgcg gcctcggtcg
    17941 atggccagat catgccgccg gtcatgggcg ctgcggcctt cctgatggtc gagtacgtgg
    18001 gcattcccta caccgaggtc atcaagcatg ccttcctgcc ggcgctgatc tcctacatcg
    18061 cgctcttcta cgtggtgcac ctcgaggcgc tcaaggccga catccgcggg ctgccgcgcc
    18121 gggtgacgaa atccgccgga catcgcctgc tgtccttcgg cctcaccgtg tcgggcctga
    18181 tcgtccttgc cggggcggtc tattacggca tgggctggat caaggcgctg gcaggcgacg
    18241 cctcggcgat catcgtcgcg gtgctcatgt tcggctgcta catcggcctg ctgtggtacg
    18301 cgacgcggca ccccgagctg cacatggacg accccagcag ccccctgacc gagctgccgg
    18361 agaccggccc gacgctgaag acgggcctgt actacctgct gccggtggtc gcgctgatct
    18421 ggaacctgat ggtggaggag ctctcgcctg cgctgtcggc gttctgggcg accctgttca
    18481 tgatcttcat cctggtcacg cagcgcccgc tcaaggcgat cttccgcaac accggcaacg
    18541 tgggtgaggc aacccggcag ggcttcgccg acctccacac cggcctggtc accggggcgc
    18601 gcaacatgat cggcatcggc atcgcgaccg cgaccgccgg catcatcgtc ggcaccgtca
    18661 cgctgaccgg tgtcggcctg gtgctcaccg agttcgtcga ggcggtgtct ggcggcaatc
    18721 tgatggtgat gctggtcatg gtcgcggtca tctgcctgat cctcggcatg ggcctgccca
    18781 ccacggcgaa ctacatcgtg gtgtcgaccc tgatggcgcc ggtcgtggtc gaggtcggcg
    18841 cgcaaagcgg cctgctcgtc ccgctgatcg cggtgcatat gttcgtcttc tacttcggcc
    18901 tgatggccga cgtcacccca ccggtcggcc tcgcctccta cgccgccgcg gggatcgcac
    18961 gctgcgaccc gatcgctgcg ggcgtgcagg ccttcctgta ctcgatgcgg acggcgatcc
    19021 tgcccttcat gttcatcttc aacacccagt tgctgatgat cggcgtcgat tcggtgtggc
    19081 tgttcgccct gacggtatcg agtgcgatcg tggcgaacct gatgttcgcg gcggccaccc
    19141 agggctggtt catcacccgc aaccgcgtgc acgagtcgat cctgctgttg ctggtgacct
    19201 tcacgctgtt ccgcccgggc ttctggatgg acatgctgta cccaccctat caggagctgc
    19261 cggcctcgga gctgacccgc ctggtcgagg aggctccacg caacgacaac ctgcgcatct
    19321 gggtggaggg gatttcgctc gagggcgagg aagtctcgaa aggcatgctg cttccgctcg
    19381 gcgacactgc gcccagcgcc agggagcgcc tgggcagggt cgggctgacc ttgatgagcc
    19441 ttggagatga agtccaggtc gccgcggtgc gcttcgggag ccagagcgag aagctggggc
    19501 tggagcaggg gtacaagatc gcccgcatcg aggtaccgtc cgtcgaccgt ccgtcgaggg
    19561 aatggatgtt cgtgcccgcg ctcgcgctgc tgggcttcgt ctggttcgcg cagactcgcc
    19621 gcgcgcgacg gcagcagccc acgcgcgcct gaaagaccgt gggggacgga cgtcgctacc
    19681 tccagagcga cgtccgtccc ccacgactgc cgggcacgtc aggaagtttc agatcagcga
    19741 cggcccctcg acgattgccg ccgcgtcctt ctcgctgatg cgcacgctcg cgttcatgat
    19801 caccaatgcg atcagccagt cgcgcaagcg ctcggcgcca tcgccctcga gcgcgcggaa
    19861 gcggcagccc gcctgcccgc tcgcagcgat gaaattcacc gcctgcgccc gaccgcggat
    19921 gtggtcggga acgccctcgc gctgcagcac gaagtcgaaa ggctcgccgc ccgccggagg
    19981 cgtttcggcg gcgatggcga aaccatcgac cgagatgtcg cgcagctcaa tgcgctcgtc
    20041 gccgatcatc acccagaacc aaggctggcc acggcgctgg gccaggaagc gctgaatgcg
    20101 gcggcgctcg gcggcctcga ccgcctgcgg cacggcgctg gcgccacgat cgctcatctt
    20161 cagccgcgct ccttgcgtac gcgggcgatc aactcgtcgg cgaccttgag cgtattctcg
    20221 tggctgccgg tgagcgaggc cgaggtcagg tagcgtccgc cgaccaccat ggacggcacg
    20281 ccctggatct tcatcgcgcg cacgagctga tcggcgcgct gcaccatcga gcccacgccg
    20341 aaggacttgt aggtctcgac aaacttggcc gcattgccca ccttgcctgc gatccattcg
    20401 gacacctgct gctcgttgaa cagcgggcgc ttgtcgtcct gcaccgcggc gaagatcgcc
    20461 ggctcgagcg cggcgagatc gccggtgatc tgcgcggcgt agaacagccg ggcgagaccg
    20521 ctcagctggg cgttgttcca caccgccggc accgcgcgga aggcgacgtc ggccggcagc
    20581 ttcttcttcc acgcggtgat cagcggatga aagtcgcggc agtgcgggca gccgtagtgg
    20641 aagaactcga gcacctcgat cttcgaggga tcgtccgcct tctgtgccgg ctcgatgatc
    20701 tggaagggct cgttcgcctg cacgaccgag gcggcgggaa gggcgagggc caggccggcc
    20761 agcttgaggg catcacgacg attcatgcgg aactcctttg actgaggctg ggtgagacgc
    20821 ggacggcgca cggacgttca atccgcagcg tgtccaggct cactggttgc ggctcaccga
    20881 ggcattgaaa cccgcatcgg agaggcgggc ccgcatcggg ttcatgtcct cgggcttggc
    20941 gaaggggccg acgcgcacgc ggtgcacgat gcgcccgtcg ggcagctggg tgcgctgcgc
    21001 gctcgcctgg atgcccgaga gcgccagccg cgccttgagg ttgtccgctt cggacgggtt
    21061 ctcgaacgag cccacctgca ggtagatgcg ctcgttgcgc tggggcgcgg gcggcgtcac
    21121 cgccgcgggc tgcggcgagg cctcgaacac cggcgcgggg gcctgctgcg cgggcacggg
    21181 cgggggcggc gcctgcacct ggggcgcggg caccggcacg acctgcgccg ccgacgaagg
    21241 cacgttgtcg ccctgcggca ggatgcggta gaactcgaaa tcctgcttgg ccaccgggcg
    21301 gtcgcccggc ttgccgggca gcgccgccgg cccctgctcc gccggcgcct gcacgcgcgg
    21361 cgccaccggc gcagcctgga agggattgct gcgggtgaag taccacgccg cgccagccgc
    21421 gatcagcgca ccgaagacga ggccgatgaa gatgccgatc agcgttccgc cggcgctctt
    21481 ggccggtgcg cgcgccggct tgcggaccgg gccgcgatct cgactcacga ttgccccccc
    21541 tgcatcacat cgactcgggc gcggacacgc ccagcatctt caggccggtg accagcacct
    21601 ggcgcaccgc cgcggcgagc gcgaggcggg cgagcttgag cgcctcgtcc tcgaccagca
    21661 tgcgctcggc gttgtaccag ctatggaagt cggcagcgag atccttgagg tagaacgcga
    21721 tctggtgcgg cgcgtagccg gcggcggcgt cctgcaccag ctcggcgaag ctcgccagac
    21781 gcgcgcacag cgcgagctcg cgctcgttct gcagcaggcc gaggtcggca ccggccagct
    21841 ccgcggcctc gccgccccac tggtcgagca ccgagcacac gcgggcgtgg gcgtactgga
    21901 cgtaatacac cggattctcg ttgctctggc tcttggcgag gtcgaggtcg aagtcgaggt
    21961 gctggtcgga cttgcgcagc acgtagaaga agcggcaggc atcgttgccc acctcgcggc
    22021 gcagctcgcg cagggtgacg aactcgcccg agcgggtgga catcgaggtc ttctggccgt
    22081 cgcggtagag caccgcgaac tgcaccaagg caacgtccag tcgggccgga tcgagcccga
    22141 gcgcggcgat cgcgcccttc acgcgcggga tgtagccgtg gtggtcggcg ccccagatgt
    22201 cgatcatgcg gtcgaagccg cgctcgtact tgttgaggtg gtaggcgatg tcggaggcga
    22261 agtaggtgaa gaggccgttc tcgcgctgca cgacgcggtc cttctcgtcg ccgaaggcgg
    22321 tggagcggaa ccacttcgcg ccgtcctgga gatagatgtg accggccttc tcgagctgga
    22381 tcaccgcgcg ctcgaccagg ccggtgtcga acaggctctg ctccgagaac cacttgtcga
    22441 agcgcacgcc gaactcctgc aggtcctcgc gaccatcgcc gagctgctcg ttgagcgcga
    22501 agccgtgcac ccaagggtag tcctcgccga gcagcttctt ggcgttggcg atcagcgcgt
    22561 cgaggtgttc ctcgcgctgt tgcttggcct cgtcgtcctt gcgatcggaa ggcggcaggc
    22621 cgggcgtccc ggccaggatc tgcatggcgg tgaccttggc gaagcggtcc tggtgggcgt
    22681 cgcgcatctc gcggcccatg tcgaccacgt agtcgccctg gtaggcgttg ggcgggaagg
    22741 gaatctcgat gccgaagaag gcgaggtagc gcagccaggt cgacagcgcc aggatgtcca
    22801 tctggcggcc agcgtcgttc acgtagtact cgcggctgac ctcgtagccg gcgaaggcga
    22861 gcacgtccga gagcgaggcg ccataggcgg cgccgcgacc gtggccgacg tggagcgggc
    22921 cggtggggtt ggcggagacg aactccacct gcaccttgat gcccttgcct gccccgcggc
    22981 cgaaatccgc gcccttggcc agcacctcgc gcaccaccgc ggtcttggcg tcggaggcga
    23041 gcgtgaagtt gatgaagccg gcgccggcga cctcggccct ggcgaccagc ttcgagggcg
    23101 gcagctcggc gagcagcagg ccggcgagct cgcgcgggtt gcgcttgagc ggcttggcga
    23161 gctgcagcgc gaggttggtg gcgaagtcgc cgtggctcgc ctgcttgggg cgctccagca
    23221 ggatgggggt ggctgccagc tccggcgcca cgctcttcag cgcagtctgg agcaggtcgg
    23281 tcagcaggac tttggggtcg gcgctcatgg cactcggaat gaattcgaaa cgcgcgatta
    23341 tagcggatcg gcgcagtccg acccggcgag gatgtgagtg caggttgcga gcccctagcc
    23401 ttcgggcgcg ctcccgcccg ttcccccgcc ggcgctttcg ggctaaagtc cgcgctttgc
    23461 ctcgtccggc ccgaccaccg tgcgcctctt ccgtctcgcc aagatcgtct ccgtcagcct
    23521 gcgcttcggc ctcgaccgca tggtgctcga cgccgacagc agtggccggc tggcgcacgt
    23581 gtggcacaag gtcttcttct ggcgcacctt ttccgagtcg cgcggcgcgc gcctgcgcca
    23641 ggcgctggag tcgctcgggc cgatcttcgt caagttcggc cagatgctgt cgacccggcg
    23701 cgacctgttg ccgcccgacc tcgccgacga gctcgccctg ctgcaggacc gcgtcccgcc
    23761 cttcccgacc gagcaggcgc tcgaagtgct ggaaggcttc tacggtcgtc cgatcgacga
    23821 ggtcttcgac gacttcgagc gcacgccggt ggcctcggcc tcggtggcgc aagtgcattt
    23881 cgcgcgcctg cccgacggca ccgacgtcgc ggtcaaggtg ctgcgcccgg gcatcgagcg
    23941 cgtcatcgag cacgacctcg ccctgctcga agtcgccgcg gtgctgctgg agaagatctg
    24001 gcccgaaggc cgccgcctca agccacgcga ggtggtcgca gagttcagca agtacctgca
    24061 cgacgagctc gacctgatgc gcgaggcggc caactgctcg cagctgcggc gcaacttcac
    24121 cgactcctcg ctgctgatcg tgcccgaggt gtattgggac tggtgcggca gcaaggtcat
    24181 ggtgatggag cgcatgcgcg gcgtgccgat ctcgcagacg ccggcgctgc tcgcccaggg
    24241 caccgatttg aaagcactct cccgtgccgg cgtggagatc ttcttcaccc aggtcttccg
    24301 cgacggtttc ttccacgccg acatgcaccc gggcaacatc ttcgtgcatc aggacgggcg
    24361 ctacatcgcg ctcgacttcg gcatcatggg cacgctcaac gaggtggaca agaactacct
    24421 cgcgaccaac ttcctcgcct tcttcaagcg cgactaccgg cgcgtggcgc tcgcccacat
    24481 cgaggccggc tgggtgccgg caaagacgcg ggtggacgag ttcgaggcgg cgatccgcac
    24541 cgtgtgcgaa ccgatcttcg acaagccctt gaaggacatc tccttcggca agaccctgct
    24601 gcgcctgttc cagaccgcgc gccgcttcga gatggaggtg cagccgcagc tcgtgctgct
    24661 gcagaagacc ctgctcaaca tcgagggtct gggccgccag ctcgaccccg agctcgatct
    24721 gtggaagacc gccaagccct tcctcgagcg ctggatggac gagcgcatgg gcgtgcgcgc
    24781 gctggtgcgc ggggtcaagg aagaggcgcc ggcctgggcc ggcaccctgc cgcagctgcc
    24841 gcgcctggtg caccaggcgc tcaccgaatc ggcccgtcac cagagcgccc agcagcagcg
    24901 cctcgaagag ctcgccgccg ggcaacgcac ccagggccgc ctgctgctct tcatcggcgc
    24961 cgtcgccatg ggcctgctcg gcctggagct ctaccgcctg ttcggctgac gcgcgccgcg
    25021 gcattcaacg ccgacgttgc gcccatgcaa taaagcgcga acgatcaagc ccttggcgcg
    25081 cgcatcccgc cccccgcggc ggcgttctgt tggtacactc gcgccctctt ttttcgctca
    25141 ccgcgcgcgg atgcgccaac acgaaaggcc caaccatgct gctctggctt gtcgtcgcct
    25201 acctggtcgt ctccatcggc atcggcctgt atgccgccac ccgggtgcac aatgcgcgtg
    25261 actacatcac cgcagggcgc aacctgccga tgatcgtcgt gctggcgatg gtgttcgcca
    25321 cctggttcgg cgccgagacc gtgctcggca tctccgccac cttcctcgag gaaggcttcc
    25381 acggcctgat ctccgacccg ctcggcgcct cggcctgcct ggtgctgttc ggcctcgtct
    25441 tcgcccgccc gttgtaccgc atgaacctgc tcacgctggg tgatttcttc cgcgtgcgct
    25501 acaaccgcac gaccgaactc gtgctgtcga tctgcatcgt gctctcctac ctgggctggg
    25561 tggcggcgca ggtcaccgcg ctcgggctgg tgttcaacgt gctctcgggc gacgcggtca
    25621 cgatgaacca gggcatgctg atcggcgccg gcgtcgtcct cgtctatacc ctgttcggtg
    25681 gcatgtggtc ggtggcgatg accaccttcg tgcagatgat cgtgatcgtg atcggcctgg
    25741 tctgggtgag ctggatcgcc ggcgacatgg cgggcggctt cgacaacgtg atcgccaagg
    25801 ccgcggccga aggcaagttc gagttcctgc ccgcgctgga cgcggtcgag atcgtcgcct
    25861 ggatcgccgc gttcgccacc atggcactcg gctctatccc gcagcaggac gtgttccagc
    25921 gcgtgaattc ctccaggaac gagcgcgtcg cgatctgggg cacgacgctc ggcggggtga
    25981 gctacttctt cttcgccgcg gtgccgctct tcctcgccta caccgccaac atcatcgatc
    26041 ccggcatggt cgagcgcctg atggagcagg actcgcagct catcctgccg acgctgatcc
    26101 tgcagtacat gcccttctac gcccaggtga tcttcttcgg cgcgctgctg tcggtcatca
    26161 tgtcgaccgc ctccggcacc ctgctcgccc cctcggtgac cttctccgag aacgtgctgc
    26221 gcggtttctt cccgggcatg ggcgaccgcg ccttcctgct cagcacgcgc gtcaccgtgg
    26281 tgctgttcac cgccttcgtc acctggtacg ccaccagcac cgaatcctcg atccacgaga
    26341 tggtcgagaa cgcctaccgc gtcaccctgg ccggcgcctt cgtgccgctc gccgccggcc
    26401 tgttctggaa gcgcgccaac aacctcggcg ccgcgctggc gatcatcctc ggcctgtcga
    26461 cctggatcct cttcgagctc ttcgtccccg aaggcgacat cgagccccag ctctacggcg
    26521 tggcggcgag cgcgctcggc atgctggcgg gtggactgat cggccggcag ccgcaccacc
    26581 gcccgcacat cggcggccac catgcggcgg cgggtaccca ccacacgcat cgctgaccgt
    26641 cccgcgcccg cccgggtgcg tgcagcccgc accggcggcc gatgcgtttg atggtgatca
    26701 acgcgtcggt cgccggtctc gtttatggtt gcggcgtttt tttcctctgg aaaccccgct
    26761 ccatgtccgt gtcttccccg atcgaactcg tctgccccgc gggcagcctc ccggccctca
    26821 agacggccat cgatcacggc gccgactgcg tctatctcgg tttcaaggac gccaccaacg
    26881 cacgcaactt ttccgggctc aacttcgatc cggcgcaggt gcgcgagggt gtcgcctatg
    26941 cgcacgcacg caagcgcaag gtgctgctgg cgctcaacac ctatccccag accgacaact
    27001 ggtcgtcctg gacacaggcg atcgaccgcg ccgccgacta cggcgttgac gcggtgatcc
    27061 tcgccgatcc cgggctcatg gcctacgccg caaagaccca cccgcagctg cgcctgcacc
    27121 tgtcggtgca gggctcggcg accagctacg aggcgatcaa ctggtaccgc gaacgcttcg
    27181 gcatccagcg cgcggtgctg ccgcgcgtgc tgtcgatggc ccaggtcgag agcgtggtcg
    27241 ccaacaccca agtcgagatc gaggtgttcg gcttcggcgg cctgtgcgtg atggtcgagg
    27301 gccgctgcgc actgtcggcc tacgccaccg gcgagtcgcc caactgcaac ggcgcctgct
    27361 cgccgggcaa gcacgtgcgc tgggaagaca ccccgcgcgg catggagacg cggctcaacg
    27421 gcatcctcat cgaccgcttc tccgaaggcg agcgtgccgg ctatcccacc ctgtgcaagg
    27481 gtcgcttcga ggtcaacgac gagacctact acgccctcga ggagcccacc agcctcaaca
    27541 cgctcgagtt gctgcccgaa ctcgcgcgca tcggcatccg cgcgatcaag atcgagggtc
    27601 gccagcgcag cccggcctac gtcgcccagg tcaccaaggt gtggcgtgcc gcgctcgaca
    27661 agttgaacag cggcggcgcc gcaggtttca gcgtgctgcc ggcgtggatg gccgagctca
    27721 acaaggtctc cgaaggccag agccacaccc tcggcgccta ctaccgcccg tggaagtgag
    27781 cctcgcgccg accgcccgcg ccgtcccgac ccgatccgat cgccgccgcg cggagcaggc
    27841 gcccgcagcg cgcccgcccg cggcgtcgag ccccccatcc gaatgagaac cgcccgatga
    27901 aactcgccct cggccccctg ctctactact ggccccacca gagcgtgctc gacttctacg
    27961 ccgacctggc cgaagccccg ctcgacagcg tctatctcgg cgagacggtg tgctcgcgcc
    28021 gccacgaact gcgcctggac gactggctgg aggtcggcaa ggttctcgcc gccgccggca
    28081 aggaggtggt gatcagctcg caggccctga tcgaatccga gtccgacctc aagaccctgc
    28141 gccgcatcgt ccggcagcac gacttccgcg tcgaggccaa cgacatgggc gcggtgcgcc
    28201 tgctcgtcga ggccggcgtg gacaactgga tcgccggccc cacgctgaac atcttcaacc
    28261 ccacgacgct gcagctcatg atcgactcgg gcgcgacgcg ctgggcggtc gcccccgaga
    28321 tgtcgggcgc ggcgctcgcc gaggtgcgcg ccgcgctcgc caggccgatc gagaccgaag
    28381 tcttcgccta cggtcgcctg ccactggcgc actcggcgcg ctgcttcacc gcccgccact
    28441 tcaacctgca aaaggacacc tgcgagttcc gctgcctgtc gatcatggac gggatcacgc
    28501 tgcgcacccg cgagggcgcg cccttcctga cgctcaacgg cgtgcagacc cagtctgcac
    28561 gggtgcacaa cctgctcggc gacctgcccg ccgtgcgcgc cagcgccgag gtgctgcgca
    28621 tcagcccgca gggcgcgcac actcgccaga tcgtcgaact cttccgcgcc gcgctcgacg
    28681 agcagcgcgg tccggcggac gcgctcgccg ccagccgcga gtggatggtc gaggagccct
    28741 gcaacggctt ctggcacggc cgcgccggcg tcgaacagta cgtcgccgcc tgaccccttt
    28801 ccgacctgct gcaaacccgg agtccgcaca tgcccgcact gcctgccctg ccccgcccgc
    28861 cgctgccttc gcacttgctg cccggcagcc tgcgccagcg catcggcgaa cgcctcgcca
    28921 ccctgcgcat ccccgacttc accgtgcccg ccccggtggc gaagctggtg tcgcgcctgc
    28981 cccagcagcc gccgacccac gtgctcacgc tcgcgctcaa cctcgcgctc gaccgcatcc
    29041 tgccgcgcga caccctcgaa ccgctgagcg gccgccacct gcagatgcgt gtgctcgacg
    29101 ccgggctgac gctcgacttc accctcggcg agcgcggctt ccgccgtgcc accccggccg
    29161 cgggcgccag accggacctg atcctctccg ccaccacgcg cgacttcatg gcgctggcac
    29221 tgcgcgagga agaccccgac acccttttct tcagccgccg cctgcgcatg gaaggcgaca
    29281 ccgatctcgg cctgctggtc aagaacacgc tcgacgcggt ggactgggac gccctcaagg
    29341 cgaagctgct cggccgccac ggctgacccc ctgcaggagc ggcgcaagcc gcgaaccggc
    29401 ccctccggcc accgccgcgc atcgaccgca gcgcgtccgt ccgcatccgc ggtaagctcg
    29461 cggtccgctg cccgacgttt gccgccatgc cctccgaatt cgacctgatc cggcgccact
    29521 tcacgcgccc cgcgcgccac accgagctcg ccgtgggcga cgacgccgcc ctgctcgcgc
    29581 cgcgccccgg catgcggctg gcggtgtcca ccgacatgct ggtggccggc acccacttct
    29641 tcgccgacac cgacccggaa gggctaggct ggaagacgct cgcggtcaac gtctccgacc
    29701 tcgccgccat gggcgccgag ccgcgctggg ccttcctcgc gctcgcgctg ccgcaggcgg
    29761 acgaggcctg gatcgctgcc ttcgcgcgcg gcttcttcgc ctgcgccgac gccttcgcga
    29821 tcgacctcgc cggcggcgac accacgcgcg gaccgctgac gctttcagtg accatcgtcg
    29881 gcgaggtccc ggccggcgcc gcgatcaccc gtggcggagc gcgcgcgggc gacgacttgt
    29941 ggatctcggg ccagcccggc cgcgccgcgc tcgggctcgc ggcgctgcgc ggcgaaaccg
    30001 gcctggacgc catccaccac gccgaattcg tcgacgccct gcaacggccg cagccgcgcg
    30061 tagcgctcgg gctcgcgctg cgcagcctgg cgagcgcgat gctggacgtc tccgacggcc
    30121 tgctcggcga tctgcgacac ctcctcgaat gctcgggcac gggtgccatc gtcgaggtcg
    30181 gggcgctgcc gctcgccgcg ctcgccgcca gcggcgccga tcccgcgctg tgccggcgct
    30241 gcctgctcgc gggcggagac gactacgagc tgctgttcgc ggccgcgccc cgacaccgtg
    30301 ccgagatcga tgccttgtcg gcccgactcg acctccccct gcatcgcatc ggcgaattca
    30361 cccccgcacg cgagcgcctg ctgctgcgcg aggccgacgg cggcctggcg gccccggccg
    30421 catgcggcta cgatcacttc gcctgaggcc cgatccggcc cacctctcga aacgaccatg
    30481 cgccccaccg cccgcctgct gatgagccac cccgcccact tcatctcgct gggcttcggc
    30541 gccgggctgt cgccgtgggc gccgggcacg gtcggcaccc tgctcgggtg ggcgatctac
    30601 ccactgatcc gcaccccggt gtcggagttc gtcttcctcg cactgctcgc cagcttcttc
    30661 ctcgccggcg tgctcgccgc cgagcgcacc gggcaagcgc tgggggtgcc agaccacggc
    30721 ggcatcgtct gggacgagat ggtggcgatc tggctggtgc tgctgttcac ccccgccggg
    30781 ctgctgtggc aggcgatcgc ggtcgcactg ttccgcttct tcgacatcgt caaaccgcct
    30841 ccggtgcgct gggccgaccg cagcttcaag ggcggcttcg gggtcatgtt cgacgacctc
    30901 gtcgccgccg gctacaccct gctggcgctc gcggtgctgg ttcacctgct ggggagctga
    30961 gaccatgacc gatcacgaac tcgacgccct cgcgcgcagg gtgggcgaag ccctcgccaa
    31021 ccgcaggctg tggctggcca ccgcggagtc ctgcaccggc ggctggatcg ccgaggccgt
    31081 caccgcgatc gccggcagct cgggctggtt cgaccgcggc ttcgtgacct actcgaacga
    31141 cgccaaggcc gacatgctcg gcgtgcgcgc cgcgacgctg gagacgcacg gcgcggtcag
    31201 cgaagccacc gtgggcgaaa tggccgcggg cgcgctcgac cacagccgcg ccgacctcgc
    31261 ggtggccgtg tccggggtcg cgggaccggg tggaggcagc cccgccaagc cggtcggcac
    31321 ggtctgcctc gcttgggcac ggcgcgacgg ccccaccggg gtcgtcaccg tccatctcga
    31381 cggcgaccgc gccgcggtgc gccggcagac ggtgatcctc gccctgcaag gcgtactcga
    31441 acgctgcgtc tgaggggcgc agggcgcacg gggccgggaa atccgaaccg ccaccctcgc
    31501 cacttcgccg gacgcagccc cgcgccggaa atccgcatga catgtttgct gtaattgcat
    31561 acaggctcta cacttacggc atcaggcctg caccccgcat gtctgcaatc tttgtgccat
    31621 aatcaggcaa tcaaacagga cgcacttcca tggacgaaaa caaggccaaa gccctctccg
    31681 cggcgctgtc gcagatcgaa aagcagttcg gcaagggttc gatcatgcgc atgggcgacg
    31741 gcagcatcga gcgcgacatc cagaccgtct ccaccggctc gctcggactc gacatcgcgc
    31801 tcgggctcgg cggtctgccg cgcggtcggg tggtcgagat ctacggcccc gaatcctccg
    31861 gcaagaccac gctcaccctg caggtcatcg ccgagatgca gaagctcggc ggcaccgcgg
    31921 ccttcatcga cgccgagcac gcgctcgacg tcggctacgc ggaaaaactc ggcgtcgaca
    31981 tcgaggatct gctgatctcg cagcccgaca ccggcgaaca ggcgctcgag atcgccgaca
    32041 tgctggtgcg ctcgggcggc gtggacatcg tggtcatcga ctcggtcgcc gcgctcaccc
    32101 ccaaggccga gatcgagggc gagatgggcg accagctccc cggcctgcag gcccgcctga
    32161 tgtcgcaggc actgcgcaag ctcaccgcca acatcaagcg caccaatacc ctggtgatct
    32221 tcatcaacca gatccgcatg aagatcggcg tgatgttcgg cagcccggag accaccaccg
    32281 gcggcaacgc gctcaagttc tacgcctcgg tgcgcatgga catccgccgc accggcacga
    32341 tcaagaaggg tgacgaggtg gtcggctccg agacccgcgt caaggtggtc aagaacaagg
    32401 tctcgccacc gttcaaggaa gcccacttcg acatccttta cggcgagggc atctcgcgcg
    32461 aaggcgagat catcgacctc ggcgtcgagc acaagatcgt cgacaagtcg ggcgcctggt
    32521 acgcctacaa gggcgacaag atcggccagg gcaaggacaa ctcgcgcgag ttcctgcgcg
    32581 ccaacccggt gctggcccgc gagatcgaga acaaggtgcg ggtcgcggtc ggcctgcccg
    32641 agctgccgcc cgccgccgcg tccgccccgg cgccggccga ggcctgaccc cgtcggcacc
    32701 gacatgggcg agccgagcct gcgcgaacgt gcgctccgcc atctcgcccg gcgcgaccac
    32761 tcgcgcgccg aacttgcgcg caagctcgca ccccatggcg agccggccga gatcgaagcg
    32821 gtgctcgagc gcatgcgcga gctcggcctg cagtccgacg agcgctacgc cgaggccttc
    32881 gtgcgcggca aggcggcgcg cttcggcacc gcacgattgc gcaacgagct cgtgcggcga
    32941 ggcatcgacc gtgaccggat cgacgaggcg ctcgccgacg agtgcgtaga atccgagatc
    33001 gagcgggcgc gcgcggtgct gcacggccgc ttcgccaccc cacccgccga cgcacgcgaa
    33061 tgggcgcgcc aggcgcgctt cctgcaaacc cggggcttcg cccccgagct gatccgcaag
    33121 ctgctgaaag agccctacga tgagtctgct taaggtcgcc ggcctgcaaa agcgctacaa
    33181 ggcgcgcacc gtggtgcatg acgtcggctt cgaggtcggc tcgggcgagg tcgtcggcct
    33241 gctcggtccc aacggcgccg gcaagaccac ctgcttctac atgattgtgg gtctggtgcg
    33301 cgccgacggc ggccagatcg ccctcgacgg caccgacctc acccacctgc cgatccacgc
    33361 ccgcgcgcgt ttgggcctgt cctacctgcc gcaggagatg agcgtgttcc gcaagctcag
    33421 cgtggccgag aatatccgcg ccgtgctcga actccagggg ctcgaacgcc gccagatcga
    33481 ggagcggctc gaggaactgc tgatggaact gggcatccat cacctgcgcg acaacaccgc
    33541 gatctcgctc tcgggtgggg agcgccggcg ctgcgagatc gcccgcgcgc tcgccaccga
    33601 cccgcgcctg atcctgctcg acgagccctt cgccggggtc gatccgatcg cggtcatcga
    33661 catccagaag atcatccgct tcctcaagga gcgcggtatc ggcgtgctga tcaccgacca
    33721 caacgtgcgc gagaccttgg gcatctgcga ccgcgcctac atcatcagcg aaggtcgcgt
    33781 gctggcgagc ggagagccta ccgagatcat cgagaacgag cgcgtgcgcc aggtctacct
    33841 cggcgagcac ttccgcctgt gagcccgccc tcctgccgac cgcgatgaaa cccagcctcc
    33901 agctcaagct ctcccagcac ctcacgctga cgccccagct gcagcagtcg atcaagctgc
    33961 tgcagctgtc tacgatcgag ctgaaccagg agatcgagcg cgtgctgctc gagaatccca
    34021 tgctcgagcg cgaggatggc gacggcggcg tcgactacgc ccccgcgccg ccgccgggca
    34081 acgcgcccga gcgcgaagcc gcgccggaac ccgccgccga accggcgggc gagtcgatca
    34141 cgcacgaggg cggcgaaggc agcagcgagg acgagggcat cgactggtcc aacgtgggtc
    34201 agggcgcgtc cagccgcagc gaggacgacg aggacggcga ctaccaggac atccaggccg
    34261 ccggcgtgtc gctgcgcgaa cacctcgacc agcaggtcgc cctctcgccc ctgtccgacc
    34321 gcgaccgcgc gctggtgcgc ttcctgatcg aggcactcga cgacgacggc tacctgcacc
    34381 agccgctgga agatctcctc gagctgttgc cgcaggacgc cgaggtcgat ctcgacgagc
    34441 tcgccatcgc cctgcgccac gtgcagagcc tcgagccggc cgggatcggc gcgcgcagcc
    34501 cgcaggaatg tctggccctg cagctccagg ccttgcccga gtgcccgata cgccctctcg
    34561 ctctcgagat cgtgaccagc cacctcgaac tgctcgcgga gcgcaacttc gcccgtatcc
    34621 gcaagctcac cggctgtgac gacgagggtc tgcgcgaagc gcaggccctg atctgcagcc
    34681 tcgaccccca ccccggctcg cgctactcca ccgccgagac gcgctacgtg ctgcccgacg
    34741 tcgtcgtgcg caagctgcgc ggccaatgga ccgtgagcct gaaccaggaa gcgatgcccc
    34801 gcctgcgcat caaccgcctg tacgcgagcc tgttgcagca gaaccgcgca cagggcggcg
    34861 gcctcggcgg gcagctgcag gaggcgcgct ggctgatcaa gaacgtccag cagcgcttcg
    34921 acaccatcct gcgcgtgtcc caggccatcg ttgaccagca acggcagttc ttcgatcatg
    34981 gggacgtggc gatgcggcca ctcaccctaa gggagatcgc agaccagctc gagctgcacg
    35041 aatccaccgt ctcgcgcgtc accacgcaga agtacatggc gacgccgcgc ggagtcttcg
    35101 agctcaagta ttttttcggc agtcatgtcg cgaccgacac cggcggtgcg gcttcctcca
    35161 cggcgattcg tgcgctgatt cgccagctgg tggacgcgga ggacagcaag aagccgctgt
    35221 ccgacgccaa gatcgccgaa attctcggcc aacagggaat cgtcgtggcg aggcgcacga
    35281 tcgccaagta ccgcgagtcc ctcaacatcc cgccggtcag cctgcgcaaa tccctctgac
    35341 aacaacagga gtcatccatg aatctgaaca tcaccgggcg ccacgtcgaa gtcacccccg
    35401 ccatccgcga atacgtgacc tccaagctcg atcgcgtcat ccgccatttc gacaacgtca
    35461 ccagcgtcac cgtcatcctg tcggtcgaaa agctcaagca gaaagccgag gtgacgctgc
    35521 acgtgcgcgg caaggacctc ttcgtcgaag cggacgacgc cgacctctac gcggcgatcg
    35581 acgcgatgac cgacaagctc gaccgtcagg ttcagaagta caagcagaag cagtccgacc
    35641 ataaccacga gtcgctcaag caccaggtcc ccgaggcctg aggaccggct tgaagctccc
    35701 cgtccggggt gtggttataa tgcgcgcgtt ttcacgaagc gcggccgcac acgccccgga
    35761 ccggattccc gcccccctgg cggcagctga cggaagagtg cgcgagccca cctgcgaatg
    35821 agcctgatat cccaactcct gccgctttcc aacgtggtga tcgatctcga cgcgagcagc
    35881 aagaagcgcg tctttgaaca ggccggcctg ctgttcgaga acaaccaggg catcgcccgc
    35941 agcaccgtgt tcgacagcct gttcacccgc gaacgcctcg gctcgaccgg actgggccag
    36001 ggcatcgcca tcccgcacgg ccgcatcaag gggctcaagg acgccgccgg cgcttttctg
    36061 cgcctggcca ccccggtgca gttcgatgct cccgacggcc gtccggtgga catgctgttc
    36121 gtgctgctcg tgcccgaaca ggccaacgaa acccatctgc agctgctctc cgaactcgcg
    36181 cagatgttca gcgaccgcga gttccgcgag accctgcacg gcgcgacgga tgccgcacag
    36241 atccaccgcc tgttcgtgaa ttggggtgcc gatgcggcaa accagcctga cccggctgta
    36301 tgaggcgcac cgcgaaagcc tgagcctcgt ccacgtcagc gggccgatcg gtgcccgtct
    36361 gtcggtcgcc gaagagcgca tctggccggc cgacctcgtc ggccacctca acctgatcca
    36421 ccccacccgg ctacaggtgc tcggcgccgc agagctcgcg tgggcgcaac gccaggcgaa
    36481 cgagaaggtc gcccaccacc tcaacgagat cctctcggcg cacccccccg cgatcatcgt
    36541 cgccgacggc tgcgagatcc ccaacatcct ccacggcatc tgcgaggcgc acgacgtcgc
    36601 gctgttcacc gcgcgccacc cctcggcgag cgtgatcgac cagctccgcc tctacctgtc
    36661 gcgcgagctc gccgagaagg tctcgctgca cggcgtgttc atggacgtgc tcgggctcgg
    36721 cgtgatgatc accggcgatt ccggcgcggg caaatccgag ctcggcctcg agctgatctc
    36781 gcgcggccac ggcctcgtcg ccgacgacgt ggtcgagctg tcgcgcatcg cgcccacggt
    36841 gctcgagggc cgctgccccg agatgctgca ggatttcatc gaagtgcgcg gcctcggcct
    36901 gatcaacatc cgcaccatct tcggcgagac cgcgtgccgg cggaagatgc gcctgcgcct
    36961 gatctgccac ctcgaccgcc gccaacccgg tcccgacgac ccctgccggc tgccgctgca
    37021 gcaggagacg caggacatcc tcggcgtgaa ggtcgcccgc gtcgtgctgc cggtggccgc
    37081 gggacgcaac ctcgcggtgc tgctcgaggc cgcagtgcgc tcgaccatcc tcaagctgcg
    37141 cggcatcgac tccacccagg aattcatcga ccgccaggcg cgcgcgctgc acgccgaaga
    37201 ggcctaccgc gccgactgag ccacccgcac ttgcggcacg gcgccgccgg ctgttacgct
    37261 cgatcgcttt gcagcagcct actggccggc catgagctcc actcccgatt acctggaacg
    37321 catcctcaac gcgcaggtgt acgacgtcgc cgtcgagacc ccgctcgacc tcgccaccaa
    37381 cctgtcggcg cgcacgcaca accgcatcta cttcaagcgc gaggacatgc agccggtgtt
    37441 cagcttcaag atccgcggcg cctacaacaa gatggcgaac ctgtcgccgc aggccttgaa
    37501 gcgcggcgtg atctgcgcct cggcgggcaa ccacgcccag ggcgtggcgc tgtcggcggc
    37561 caggctgggc gtgcgcgcgg tgatcgtgat gccgagcacc acgccgcaga tcaagatcga
    37621 cgcggtcaag gcgcgcggcg gcgaggtcgt gctcgcaggc gaatcctatt ccgacgccta
    37681 cacgcacgcg ctcgagctcg agaagagcga gaaactcacc ttcgtccacc ccttcgacga
    37741 ccccgacgtg atcgccggcc agggcacggt gggcctggag atcctgcgcg cgcacccgaa
    37801 gccgatccac gcggtgttct gctgtgtcgg cggcggcggg ctgatcgccg gcgtggcggc
    37861 ctacatcaag cgcctgcgcc cggagacgaa gatcatcggc gtcgaggcgg tggacgccga
    37921 cgcgatgacg cgctcgctcg ccgcgggcaa gcgcatcgcg ctcgagcagg tcggcatctt
    37981 cgccgacggc gccgccgtca aggaggtcgg tgccgagacc ttccgcctgt gccagcagta
    38041 tgtggacgag atgatcgtgg tcgacaacga cgccatctgc gccgcgatca aggacgtctt
    38101 cgaggacacc cgctccatcc tcgagcccgc cggcgcgctc gccgtcgccg gcgccaagga
    38161 gtacgcccgc cgcaacaagc tgcgcgacaa gagcctggtg gcggtggcct cgggggccaa
    38221 catgaacttc gaccgcctgc gcttcgtcgc cgagcgcgcc gagctcggcg agcagcgcga
    38281 ggccgtgctc gcggtgacca tccccgagaa gccgggctcc ttccgcaagt tcatcagcgt
    38341 gctcggcaac cgcaacatca ccgagttcaa ctaccgctac gccgacgccg agcgcgccca
    38401 catcttcgtc ggcgtcaccg tgcacaaccg cagcgaggtc ggacgcctgg tggagatgct
    38461 cgagcgccac gagctgcctg cgctcgacct caccgacgac gagatggcca agtcgcacgt
    38521 gcgctacatg gtgggcggac gcgcgccaca ggtggaaaac gaggtggtct accgcttcgt
    38581 cttccccgag cgcccgggcg cgctgatgaa tttcctctcc aacctgcgct cggactggaa
    38641 catctcgctg ttccactacc gcaaccacgg ctccgacttc ggccgcgtgc tggtcggcat
    38701 gcaggtgccg ccggccgaca aggacgcctt cgacgccttc ctgcagcggc tcggctacga
    38761 ctacgtggcc gagaccgaca accccgccta ccgcatgttc ctgtccggga gctgagcccc
    38821 gcaggcaggc cgtgcagggt tgcagccgcc gctcaggcgc gcggcttcgc cacccccgcc
    38881 tcggtcacca gccagtccat cgggcgatcg aactcctgcg gcagcgtgtc gggcacgcgc
    38941 ccgacctcga agcccacgcc caccgccacg gtgcgcagca ccgccagcgt gcggtcgaag
    39001 tagccccctc cgtagccgag gcggtagccg cgttcgtcga aggcattgag ggggatcagc
    39061 accagctcgg gctcgaccgc ctcgccgtcc gccgggtggg ggatgccatg gcggtcgagc
    39121 ggcatcggca cgcccggcgt ccacgcgcgg aacatcatcg gcgcatggcg ctcgagcacc
    39181 accggcaagg ccgccacccg cccgggcgcg gccgcacgcc aggcctgcac ccacgggcgc
    39241 aggtcgggct cggcgcggta gggccagcag aaggcgagcc ggcgtggctg caagcgtccg
    39301 accaggtcgt cgagatgctc gccgattgcc gcggcgaggc gctcgcgacg ctcggcaggc
    39361 aggcgctcgc gcaacgccag cgcatgttcg cgcatgcgct ggcgacgcgc gccgggatca
    39421 tgggacaagg gggcgaaact catcttcgat atggtatggt tggcggtcaa acggaggcat
    39481 cacctggcat gaagaaggca ctggttgcgg cgctcacggc cgcgacgttc ctcgcccccg
    39541 ccggcgcgtt cgcgcagatt ggcggcgagt ccggggacgg gcgtatcgtc gccgctcgcg
    39601 aggccctgcg caagggcgac cgcagcacgc tcgaacgcct cgcagccgtg cgcgaggccc
    39661 accccctcga cgcctatccg cgctactggc tgctgatcaa ccgcctcgcg cgtgccgaag
    39721 aacccgtgcc cgtcgcggcc ctgcaagcct tcctcgccga agaagccggc tccaacctcg
    39781 ccgaacgcct gcgcgccgac tggctgcgcc gcctcgccag ggacggcgac tggaacggct
    39841 tcctcgcggt gtacgcggac ctgcgcagcc ccgacgccga attgcgctgc aacgcctgga
    39901 gcgcgcgcgt gctgaccggc gagcgcagcg tcttcgccga gctcgcgcgc gaatgggaca
    39961 ccctcgccga cgccgatccg acctgcgaca cgccgctgcg cgcggcggtc gactccggcg
    40021 cggtggacga agaacgcgtg tggtggcgca tccgccgaca gatcgacacc cgcaagcccg
    40081 aggctgcgct cgccagcctg tcgctgctgc ccgccggcaa cgccccggcc cacgccgacc
    40141 tggcgcaggc gatccgctcg cccgcaccct ggctggatcg gctgcagccc aacttcgccg
    40201 tgagccgcgc cggccgcgag ctcgcgctcg ccgcactggt gcgcctggcg cgcgaggacg
    40261 tctccgccgc gcgcctgcgc ctgctgcgca tccaggaccg cctcggcgcg gccgagcgca
    40321 actacgccca tctggcgctc ggcatgcacg ccgcgctcga ccgcctgccc gaggcgagcg
    40381 cgctctacgc cgccgccggc gacatcgaga ccaccgccca gcaacgtgcg tggcgggtgc
    40441 gtgcggcgct gcgcatcggc gactggcacg cggtgcgcga ggcgatcgaa gccatgcccg
    40501 ccgacgaacg cgaagctgcc gactggatct actggctggg ccgcgcacac gccgcggccg
    40561 ggcgccgcga cgccgccgag tcgctctacg tgcgtatcgc cggcgagccg cacttctacg
    40621 gcatgctcgc gcgcgaggag ctcggcgagg ccttcccgcc gccccccgcc accgcgccgc
    40681 taccgcgcgc caagctcgag gaggccgagc gcgaccccgg cctgcagcgc gcactcgcgc
    40741 tctaccggct ggagctgcgc accgaggcgc tgcgcgaatg ggtctggggc gtgcgcgaac
    40801 gcgacgagca gttccgcctc gccgccgccc accttgcgct gcgcaacgag ctctacgacc
    40861 gcgcgatcaa taccgccgag ctggccaacc cgcgcagcaa cttcgagctg cgcttcctga
    40921 ccccctatcg cgacctcatc gagccgcagg tgcgcgcgca ggggctcgac ctcggctggg
    40981 tgtacggcct catgcgccag gagagccgct tcgtggttcc ggcacgctcc agcgtcggcg
    41041 cccagggcct catgcaggtg atgcccgcca ccggcaagtg ggtggcggat cggatcggcc
    41101 tcgccggcta caaccagcgc ctgctcaccg accccgagac caacgtgctt ctgggcacca
    41161 gctacatgcg tctcatcctg gaggggctcg acgcccaccc cgtgctcgcc agcgccggct
    41221 acaacgccgg ccccgggcgg gcgcggcgct ggcgcgacgc ggcgccgctc gagggcgcga
    41281 tctataccga gacgatcccg ttcgacgaga cccgcgacta cgtcaagaag gtgctcgcca
    41341 acgcggtgat ctacgcggcg atgctcgaaa aacggccgca atcgctcaag gcgcgactcg
    41401 gcacgatcgc gcccggggct gccagcgagt aagcttccgc gtgcgccatc ccattccatc
    41461 cacagcaaca gcacggtcca gtcgaaacat gaacccgaaa cgcgtcgtca tcgtcggcgg
    41521 ctccggcttt atcggcagcg ccatcgccaa tcgtctctgc aaggaaggcg tcaaggtcct
    41581 cattcccacc cgccgccgca gccgcgccgg ccatgttctg ctgctaccca acgtcgaggt
    41641 ggtggagggc aacgtgcatg acgtcgccct gcttgcgcgc ctgttcgagg gcgccgacgc
    41701 cgtggtgaac accgtcggcg tcctgcattc gcgcccgggc aggcccttcg gccccgactt
    41761 cgcgcgcgcc cacgtcgagc tgccgcagaa gatcgtcgcc gcctgtcgca cggcgggcgt
    41821 gccccgcctc gtgcacatca gcgccctcgg cgcctattcc gacggcccgt cggaatacca
    41881 gcgctcgaag gcggccggcg agaccgcgat ccgcaccggc tcgcccgaga tcgcgtggac
    41941 catcctgcag ccttcggtgg tgttcggccg cggcgacagc ttcctcaacc tcttcgccga
    42001 cctcgcgcgc atgttcccgg tgctgccgct cgccggcgcc accgcgcgct tccagccggt
    42061 gtatgtcgag gacgtcgccg aggcggtgtg gcagagcctg acgcgcgacg atgcaggcgg
    42121 gcagaccttc cagctcgccg gaccgaccgt ctacacgctg cgccagctcg tcgagtacgt
    42181 ctccgcgctc gtgggcaagc cgcgcccgat ctatccgctg tccgagaagc tggcgatgtg
    42241 gcaggcgcgc ctcatggagc tcgcccccca gcccctgatg agccgcgaca acgtgcgctc
    42301 gatgaaggtc gacaacgtcg ccagcggcac cccgcaaccc ttcggcatgg tgcctaccgc
    42361 gctcgagacc gtggccccgg cctggctcgg ccaggcggcg ccgcgagccc gttactaccc
    42421 cttccgccgc cacgcgcggc gctgaagagc aactctctac ggagtccgcg atgaagctga
    42481 tcatcggcaa caagaactat tcctcctggt ccctgcgcgc ctggctggct gcgcgcgagg
    42541 gcggcttcac cttcgaggag atccacatct ccctgttcat ccccggcagc cgcgagcgca
    42601 tcctgtcgca ctcgccctcg ggcaaggtac cctgcctgaa cgaccacggc gtggtggtgt
    42661 gggactcgct cgcgatcggc gaatacctcg ccgagaagcg ccccggtctg ctgcccgccg
    42721 acccggtcgc acgcgcgctg gcgcgctcgg tcaccgccga gatgcactcc ggcttccaga
    42781 acctgcgcag ccgcatgccg atgaacgtgc gcaaggacta caccggtttc ggccacagcc
    42841 ccgaggtcga tgccgacatc gcgcgcatcg tggcgatctg gaacgactgc cgcgcgcgct
    42901 tcggcgccgt gggcccctac ctcttcggcc gcttcagcct cgccgacgcg gccttcgcgc
    42961 cggtggcctt ccgcttccag acctacgcgg tgaagcccgc gggcgcggcc ggcgagtacc
    43021 tcgccaccct gctcgccaac ccgcacatgc aggagtgggc gacggcggca cgcgccgaga
    43081 ccgagtcgat cccggaagag gacctctacg gctgatgcag gcctatgtcg tgggcggagc
    43141 ggtgcgcgac gccctgctcg gcctgccggt gaaggatcgc gactgggtgg tggtcggcag
    43201 cacgcccgag gagatgctcg cgcgcggctt ccgccccgtc ggccgcgatt ttccggtctt
    43261 cctgcatccg cacaccaacg aggaatacgc gctcgcgcgc accgagcgca agtcgggcca
    43321 cggctacacc ggcttcgtct gccacgcctc gcccgaggtc acgctcgaag aggatctgct
    43381 gcgccgcgac ctcaccatca acgcgatcgc gcgcgccgag gacggccgcc tgatcgaccc
    43441 ctgcggtggc cggcgcgacc tcgccgcgcg cgtcttccgc catgtgtcgc ccgccttcgc
    43501 cgaggatccg ctgcgcatcc tgcgcgtggc gcgcttctcc gcgcgcttca ccgacttcga
    43561 gctggcaccc gagaccctgg aactgatgcg cgcgatggtc gtggccggcg aggtcgacca
    43621 tctggtcgcc gagcgcgtct ggcaggaact cgcgcgcggg ctcatggagg cgcgcccctc
    43681 gcggatgatc cgcgtgctgc gtgcctgcgg cgcactcgca cgcctgctgc ccgagctcga
    43741 ccgcctgttc gggatcccgc agccggagaa gtaccacccc gagatcgaca ccggcgagca
    43801 cgtgctgatg gtgctcgacc acgccgccgc cgcggacgag ccgctcgcgg tgcgctgggc
    43861 ctgcctgctg cacgacctcg gcaagggcga cacgcccgcc cacctgctgc cgcaccatta
    43921 cggccacgag gccgcgagcg cgcgccgcgc gcgcgaggtg tcggaacgcc tgaaggcccc
    43981 cgccgactgc cgcgacctcg ccgagatggt ggcgcgcgag cacggcatcc tggcgcgcgc
    44041 gcacgagctg cgcgccgaaa ccatggtcaa gctgatcgaa cgctgcgacg gcctgcgccg
    44101 tccgcagcgc ttcctcctga tgctgcaggc ggccgcctgc gaccacgccg gccgcggcag
    44161 cggcgcggaa aacgcgtcgg aatggagacc cttgtcacgc tggcaggccg cgctgcaggc
    44221 cgtgcgcgcc gtcgatgccg gtagaatcgc aaccgagtgc agcgacaaat cgctgattcc
    44281 tcaggccttg cacaccgccc gcgtggcggc cgtgaaggcc ctggacaagg accccgcatg
    44341 aacgcaaccc gcttcggcga actcgaggtg ctgtgtcacg cacccgagcg cgccacccat
    44401 gaacaccccc tgctcttcat ccacggcgcc tacgtttcgg catggtgctg ggacgagcac
    44461 ttcctgccct ggttcgcccg ccgcggctgg caggcctatg ccgtctcgct ctccggccat
    44521 ggccgcagcc gccagcgcga gcacctcgac tcctactcga tcgacgacta cgtgcgcgac
    44581 gtcgccgaag tcgccgcccg cctgcctgcg ccgccggtgc tggtcggcca ctcgatgggc
    44641 gggatggtgg tgcagaagta tctcgagcaa cacgacgcgc ccgccgcggt gctgatgtcc
    44701 gccgtcccgc cgcaaggcct gatgggctcg gccttcggcc tgatcttcaa gcgcccgcac
    44761 ctgctctccg acctcaaccg catcatgtcg ggcaacgacg tggacatcga cagcctgcgc
    44821 gaggcgctct tccatcagcc ggtggcggcc gacgacctgc aacgctacta ccgcctgagc
    44881 cagcccgagt cgcaccgcgc gatctgggac atgtcgctgt tcaacctgcc gcagccggcg
    44941 cgcatgcacc gcgtgccgat gcagatcctc ggcgccgagc acgacgccct gatcccgccc
    45001 gaccaggtgc acatgaccgc catgacctac ggtcgccagg ccgagatcct ccccggcatg
    45061 ggccacggca tgatgctcga gcacgactgg gagatcgccg cgatgcggat cgccgactgg
    45121 ctggccgagc gcgcgctgta gccgacgccg ggccgcctca gagcgcgtgc gtgcgatcgc
    45181 cgcgcgcgaa gccgagcagc ggtccgtcga tcgcgcgcga cagcgcgatc accttgaaga
    45241 gctcccccat ctcctgcggc gcggtcaggc gctgcaccgc gcgcgcggcg cggatgtagt
    45301 ccgcgctctc gcgggcgccg cgccgctcca ggcattccag cacgccgcag ttgaagagga
    45361 actgcgcctg cgtggtgtag ccctgcacgt ccagcccggc ctcgaagccg gcctcggcca
    45421 ccgcggtgaa gtccacgaag gcggtgatgt cgttgagccc cggccacagg aaagggtcgc
    45481 cgtgggcatg gtggcggtag tagcacagca gggtgccgcc cgaacgcgag ggcaggtagt
    45541 actcggcgcg cggatagccg tagtcgatca gcagcagggc gcccgcgtgc aggcgctcgg
    45601 cccaggccgc cacccaggcc ttgccggcga ggttcagctc ggtgacgtat tccccgctct
    45661 gcggcaccgg cagggcgagc gcccgcgctc cttccgccac cgcgcccgcc gccggcacgt
    45721 ccgcccagca cagccggcgt atccccgcgg catcggtggc gatggcgacg ccgcgctcga
    45781 agagcccctc ggcgcgcgac accagcagat gcaccggcat cacgtcgagc acctcgttgg
    45841 ccaccactgc accggagaag cgctccggca gcgcgtccag ccactgcacg cgcgccgcca
    45901 ggtgaggaac cttggcggcc agggtgtcga actggcgttc gcgcaattcg cccgagagct
    45961 cgaggatgcc gtagcgctcg ggcaggcagc cgcggcgttc gagctcgagc agcaggtcgg
    46021 cggcgagcag gccggtaccg gcgccgacct cgatcagcgc gggcgcgctc gcgcgcatca
    46081 cctgctcgac ctgggcggcg agcgcctggc cgaaaagcgg ggtaagctcg ggcgcggtga
    46141 tgaaatcgcc gccggggccg aactttcgcg cgccaccgct gtaatacccc agccccggcg
    46201 cgtagagcgc gagctccatg tagcgcgcga acgggatcca gccagcggcc gcggccagct
    46261 cggcctcgat gtgttcgagc aggcgggcgc tctgggcgag cgcatcggcg gaaggctggg
    46321 gcaatgaaga catgatcgtt ctgcaggaca ggagggcgag gccgcgattg tagcggcacg
    46381 cccgcccagc cgcccggcca gctccgggcc tcacggtgaa tccgatgcgc acgtctcctc
    46441 ccgaacccga atccaccgcg gcaccgctga tcctggtcac cggcgccgcg cgccgcgtcg
    46501 gcgcggcgat cgcacgccgg ctgcacgccg gaggcgcccg cctcgccctg cactaccgcc
    46561 acagcgcggg cgaggccgaa gcgctcgctg ccgaactgtg cgccctgcgc gccgattccg
    46621 cctttaccgt gggcggcgac ctcgcgcgct tcggcgccgc cgcggagatc gccgcagcgg
    46681 tgctggcgcg cggcaatcgc ctcgacggcc tggtgaacaa cgcctccagc ttcttcccca
    46741 ctccgctcgg cagcgtggac cgcgcggcct gggacgagct catgggctcc aacctgatgg
    46801 gcccgctctt cctcacccag gcgctcgcgc ccgccctgcg cgaggcgcgc ggcgcgatcg
    46861 tcaatattgt cgacatccac gcccagcgcc cgctcgccgg ctacccggtg tattgcgcgg
    46921 cgaaggcggg actggcggcg ctgacgcgtt cgttcgcgat cgagctcgcc cccgcggtgc
    46981 gggtcaacgg cgtctcgccc ggcccgatcg aatggccgga ggacgaccag ttcccgcccg
    47041 ccgagcgcga gcgcatcgtc gcccacacct tgctcggccg cgtcggcagc ccggacgaca
    47101 tcgcgcgcac ggtcgccttc ctgatgttcg acgcacccta catcaccggc cagatcctcg
    47161 cagtcgatgg cgggcgcagc gcgcatctgt agccaagggt cgtttccgcg ccggtttctg
    47221 ggataattcg cacctgattt tcccgacgag catcgaccgt gaccgcagcc tccgcccccg
    47281 ccgccgccct ccccaccacc gaggccggcg gagacgcccg cttctccaac accttcctgc
    47341 gcctgaagaa gaagctcgag cgtggcgtcg gcgaggccat cggcgactac aacatgatcg
    47401 gcgacggcga caccgtgatg gtgtgcgtct ccggcggcaa ggactcctac accctgctgt
    47461 cctgcctgct cgcgctgcgc gaacgcgccc cggtcgactt ccgcatcgtg gcgatgaacc
    47521 tcgatcagaa gcagcccggc ttccccgacc acgtgctgcc ggcctacttc gagtcgatcg
    47581 gtgtcgagta tcgcatcgtc accgaggaca cctactcgat cgtcaaggac aagattcccg
    47641 agggcaagac cacctgctcg ctgtgttcgc gtctgcgccg cggcatcatc taccgcaccg
    47701 ccacggagat cggcgccacc cgcatcgcgc tcggccacca ccgcgacgac atgctcgaga
    47761 ccctgttcct caacatgttc ttcggcggca agatcaaggc catgccgccc aagctggtca
    47821 gcgacgacgg caagcacgtg gtgatccgcc cgctcgccta ctgcaccgag gccgacatcg
    47881 cacgcttcgc gcgcacgatg gacttcccca tcatcccgtg caatctgtgc ggctcgcagg
    47941 aaaacgccca gcgcaagctg atcaagaaca tgctgcaggg ctgggcgcgc gactacccgg
    48001 gccgcatcga atcgctcgcc acttcgctga agaacgtggt gccctcgcac ctgtccgacg
    48061 cgcgcctgtt cgacttcgtc ggcctcacgc agcacaccgt ggtcgaagaa ggcgacaccg
    48121 ccttcgatcc gcccgagctg cccgaccgta cgctcggcgc cagcgtgaag ctgttccgcg
    48181 cgggcgacca ggaagaacgc gaatgcgcct gagggaggcg cgaccgcgcc tgattcggat
    48241 gccgacgccg gcgccttgcg gatccggtgc gacatcccca tgatcctgtt ccgggccggc
    48301 cacgccagcc gccagatctg acacggaaaa ccctgcgatg cccgagcgaa agaacctctg
    48361 cgtgctggtc gcgctccatc cggtggatgc ggtgctcgcc gaacgcctcg gtgcggacga
    48421 ggcgcgccat gccgccgagc gtcacgcgcg gcggatcgag cgtgcgatcg tgatgcaggg
    48481 cggccaggtc ctgctcggcg gcgcggagca gaccatcgcc ggattcgaac gcagcgacgc
    48541 ggcggtgcac gcggcgagcg aggcgctcga ccgcatacac ggccttccac ccctgctcgg
    48601 cagcaagcag acggtacgca tcggcgtgca ctatggggtc ctggacaacg atggcgagga
    48661 gcccgccgga gagggtccag cggtggcccg ccagctcgcc catctggccg agagcgggca
    48721 ggcgctgatc tccggcaccg cggtgatggt cctcggcggt gccgcacggc aacaggtcgg
    48781 ctcgcagccg ctgagcggtc cggtgtggac gcgggtcggc atcccggtgc atgcgatcgg
    48841 acagcgcagc ggtctggtca cctcggttcc cggcgcagcg cggctgtcga gccggctgcg
    48901 cctgcgccac cagcaggaag tcctgttcgt ggaggagttg cgcccggtgc tgctgctcgg
    48961 ccgcgagctg gccaacgacg tcacgatcat ggatccgcgc gcctcgcgtc agcatgcgcg
    49021 catcgagcgc cgccccgagg gcttcgtgct gttcgatcag agcaccaacg gcagcttcgt
    49081 cgccatcgag ggcaagccgg agtgcttcgt gagacaggag cagatgctgc tcgacggtcc
    49141 cggccggctc ggctgcggct tctcgacgac cgagctcgag cgcgacctgg tgttcttcga
    49201 catcgtctga gccccgcgcg cgggagaagg gcgtgcgcgg gggacacagg cgcccgcccg
    49261 cgcggcacgc ccgcatggaa acgaccggat cagccgctca tgcattcgcc gatgaaggcc
    49321 ttgcgcttgg ccccgcgcag cgactgctcg gtggccgcac gattgcaggc cttctgcctt
    49381 gccatgtcct cgctcgagac cttctgtgcg gagcccgctg tcggcgcgac cgcaagcgca
    49441 ggtgcggctg caccggccgc gctcgcggcc gtcgccgcgg ccgccggtgc gacggccggg
    49501 gccgctgccg gcaccatagc cggtgccgca acgggcgctg cagccgggac gccggccgcg
    49561 ctgccggagg cgagagcagg cgcggcaccc ggagcactcg cagcggcagc ctcgtgcttg
    49621 ccgctcaggc aggtgctcat gaaggccttg cgcgcatcgc ccccgagcgc ctgcgtcttc
    49681 gcttcggcat tgcaacgctt catgcgttcg tgctggggat tggcccatgc ctgcgcgggg
    49741 gtcagcgccg cgagcacgag aacgacgccg gtggcgccga gaattcgctt catgatcttg
    49801 tctcctcgtc cggttctacg caaaatgcgt aacacgattt tgcatatgac atacatcggc
    49861 gtcaatgcgc atcgcattgc caccgagacg catgcggctc cccgccggcg cccgcaccga
    49921 tgctgaacga ggactccacg ctcctcgcgg gcctcgccgc gtccgccttc ctggccgcga
    49981 ccctgctgcc gggcggctcc gaagcggtat tcgccgcgct gctggcgctg cgcccggaac
    50041 tgacgccggc agcgctgctc gtcgccaccg cggccaacac cgcgggcggc atgagcacct
    50101 acgcgctcgg ccgcctgctg ccccgcaagg aatccggcgc gcatgcacgc acgctgctgc
    50161 gctggggcag cctcgccctg ctgctgtcct gggtgccgct gatcggcgac gcgctgtgcg
    50221 ctgccgcggg tgcgctgcgc ctgaacgcct tcgcctgcct ggcgtggatg gcgctcggca
    50281 agggcctgcg ctacgcggcg atcgcctggg cattgccgtc cgccggcgcc tgagcccgcc
    50341 gcgcacgccg acccgcgccg cgccgccgcc ctcgcctata ctcgccccct gccctcgcaa
    50401 cggagccccc ccatgtccgc catcgaacgc cacggcgtca ccgcccgcta tgccgacgcc
    50461 gtcatccacg ccggcgtcct gcacctggtc gaggtccccg cgagcagcgc cggatcgatc
    50521 gaagcccaga cacgcgaggt gctggagtcc gtcggccgcc agctcgccaa gctcggcagc
    50581 gaccccgcgc gcatcgtgat ggccaccatc tacctcgtcg acatggccga ctacgccggc
    50641 atgaacgcgg tgtgggatgc ctgggttccg cccggcagcg ccccggcacg cgcctgcgtc
    50701 caggtcgccg ggctcgccga cccgggctgg cggatcgaga tcgcgctcac cgccgcgcgc
    50761 tgaagctcgc cgcaccaggc gacggaggcc gaatccctgc tggccttaag gtcttgattt
    50821 ttgctgcatg gtgcactgcg gttcgctaga atggaccgct tccctgcagc cgcttccggg
    50881 tagtccaatg agccaacgcc tgcgcgagat tccgtacaac tacacctcgt tttccgaccg
    50941 cgagatcgtc atccgcctgc tcggggccga ggcctgggcg gtgctcgacc agctccgcac
    51001 cgaacgcgtc accggccgct cggcgcggat gctgtacgag gtcctcggcg acatctgggt
    51061 ggtgcgccgc aacccctacc tcgaggacga cctcctcacc tcgcgcgagc gccgcgacgc
    51121 gctgatcggc gcgctcgacc accgcctcaa cgagatcgac aaacgccgcc agggcaacga
    51181 ccacgtcacc ctgctggtgg cgcgtgcgcg cgaggcggtg tccaacttcg agcgctggtt
    51241 cgacgacacc gcgcgccgcc gcaaggcggt gctcaaggcg ctggccaggc acacccggcg
    51301 cgacaacatc tgcttcgacg gtcacgcccg cgtctcccac gtcaccgacg ccaccgactg
    51361 gcgggtcgag tatcccttcg tggtgctcta ccccgacacg gaggaggaga tggcgccgct
    51421 ggtgcgcgag tgcatcgcgc tcggcctgac catcatcccg cgcggcggcg gcacgggcta
    51481 caccggtggc gcggtgccgc tcgatgccag ctcggtcgtc atcaacaccg agaagctgct
    51541 cgccatgagc gcggtcgagc ccaccgtgct gccaggcatc gacggcgcga tggccgagcc
    51601 ctaccccacc atccgcaccg gcgccggcgt ggtcaccgag cgcgtctccg aggcggcggc
    51661 gctcgccggc cgcgtgttcg cggtcgaccc gacctcggcg agcgcctcct gcatcggcgg
    51721 caacatcgcc atgaacgccg gcggcaagaa ggccgtgctg tggggcaccg cgctcgacaa
    51781 cctggcctgg tggaagatgg tcacgcccga tggcgactgg ctcgaagtcg aacgcctcga
    51841 ccacaacttc ggcaagatcc acgagcagga aaccgtacat ttccgcctca agcgcttcaa
    51901 ccccaagggc gacaagctgc tgtcggaaga aatcctgagc ctgcccggcg ccagctgccg
    51961 caaggtcggc ctgggcaagg acgtcaccga caagttcctc ggcggcgtgc cgggcgtgca
    52021 gaaggaaggc accgacggcc tcatcgtggc cgcacgctgg gtgctgcaca agatgccgcc
    52081 ggtcacccgc acggtgtgcc tggagttctt cggccaggtg cgcgaggcgg tgccggcgat
    52141 cgtcgagatc accgatttct tcaagccggg cggcgccggc cacgccgcgg gcgtgcagct
    52201 cgccggcctc gagcacctcg acgagcgcta cgtcaaggcg gtcggctacg ccaccaaggc
    52261 gcgccgccac ggccgcccca agatggtgct gatcggcgac atcgtcggcc acgacgagga
    52321 cgcggtgatg aaggccgcct ccgaggtcgt gcgcatgacc aacgcgcgcg gttcggaggg
    52381 cttcatcgcg gtcagccccg agcagcgcaa gcgcttctgg ctggagcgct cccgcaccgc
    52441 ggcgatctcg cgccacacca acgccttcaa gatcaacgag gacgtggtca tcccgctgcc
    52501 gcgcatgggc gagtactgcg acggcatcga gcgcatcaac atcgagctct cgctgcagaa
    52561 caagatcgcg ctctgcgacc gcctcgccga gttcctgcag ggcgagctgc cgctcgagat
    52621 caaggacgag ggcctcgacc gcgaggcgct ggtctccgac caccgcgccc aggccctcga
    52681 gcaggtcgcc ggcgtgcgcc ggcgctggca gtggctgctg gacaagctcg acctcgcgct
    52741 cgccgaggcc gagccgcagt tcgccgccta tggcgtccag gccggcgagc tctccaaccg
    52801 cgccgccagc ccgaccctgt tccatcgcct gcaggactac tcgatccgcg tgtcgtggaa
    52861 ggaggagctg cgcccgctgc tcgagcagac cttcgacggg gccctgttcg cccccatcct
    52921 cgcccgcatc gccgagatcc accgcgaggt gctgcgcggc cgcgtcttcg tcgccctgca
    52981 catgcacgcc ggcgacggca acgtgcacac caacctgccg gtcaactccg accactacga
    53041 gatgctgcag accgccaacc gcgcggtcga ccgcatcatg gcgctggcac gcagcctgga
    53101 cggggtcatc tccggcgagc acggcatcgg tatcaccaag ctcgactatc tcaccgacga
    53161 cgagatggcg aacttctgga gctaca
//
LOCUS       DAOQBS010000006        52168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_284_length_52168_cov_16.251415, whole genome shotgun sequence.
ACCESSION   DAOQBS010000006 DAOQBS010000000
VERSION     DAOQBS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 52168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52168
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_284_length_52168_cov_16.251415"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PKN25_01405"
     CDS             complement(<1..503)
                     /locus_tag="PKN25_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNV89581.1"
                     /translation="MFEKILIANRGEIALRILRACRELGIKTVAVHSEADTHAKYVRL
                     ADESVCIGPPPSAQSYLNVPAIISAAEVTDAEAIHPGYGFLSENADFGERVEQSGFVF
                     IGPRPETIRLMGDKVSAKDAMKAAGVPCVPGSDGALPEDPKEIVKIARAVGYPVIIKA
                     AGGGGGRG"
     gene            complement(511..963)
                     /gene="accB"
                     /locus_tag="PKN25_01410"
     CDS             complement(511..963)
                     /gene="accB"
                     /locus_tag="PKN25_01410"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNV89582.1"
                     /translation="MDLRKLKKLIDLVQESGISELEVTEGEEKVRIAKHTSAPPATYY
                     AQVPPAPAAAPAAVVASPALTDAEDELPDGHVVKSPMVGTFYRAGAPGAKPLVELGQK
                     IATGDRLCIIEAMKLMNEIEADASGVVKAILVENGEPVEYGQPLFVIG"
     gene            complement(1409..2032)
                     /locus_tag="PKN25_01415"
     CDS             complement(1409..2032)
                     /locus_tag="PKN25_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89583.1"
                     /translation="MPSHAHVSRSGGLRREIAALAARMMAEDGISDFGFAKRKAAKQL
                     GATDADALPNNNEVEAELRAWLAIYQDEEQPQRLLEMRSAAIEVMRLLADFRPYLTGG
                     ALDGTAGRYSELEIELFPESTKDVEIFFLNQDFAYEHREPRRPAPHTPEAILSFDWDD
                     VPVKVSIYPSAAERSPRRGQDRARLAQVEALVAAAVESTRDTGSHAP"
     gene            2116..3462
                     /gene="mpl"
                     /locus_tag="PKN25_01420"
     CDS             2116..3462
                     /gene="mpl"
                     /locus_tag="PKN25_01420"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HNV89584.1"
                     /translation="MHIHILGICGTFMGGVALLARAAGHTVTGCDANVYPPMSTQLEE
                     QGIGLIEGYDTSQLDLEPDMYVVGNAVSRGNPLLEAILDRGLPYVSGPQWLAEHVLAG
                     RWVLAVAGTHGKTTTTSLLAWMLEDAGLSPSFLVGGVPQNFGVSARLTESPFFVIEAD
                     EYDTAFCDKRSKFVHYRPRTAILNNLEFDHADIFADLGAIETQFHHLVRTIPASGRIV
                     ANAHEDSLKRVIERGCWSELEWFGGDGRWTVGTGADEAEAVFRLDGVEQGRVRMALAG
                     SHNRDNALAAIAAARHVGVTPAQAIASLGRFAGIKRRLELRGTVRGVSVYDDFAHHPT
                     AIALTVGGLRRREPQGRILAVLEPRSNTMKLGVMKAQLPASLAEADLVFCHAHGLDWD
                     AAAALAPLGARARTYDRLDALVAAVVAAAQPGDHVLVMSNGGFGGVHQKLLDALGA"
     gene            complement(3524..4183)
                     /locus_tag="PKN25_01425"
     CDS             complement(3524..4183)
                     /locus_tag="PKN25_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNV89585.1"
                     /translation="MTRPQHADRPAKSSRRTLLLGLSAAIALPLLQGCFPVVATGVGA
                     GAAMVSDRRTSGTYVEDEGIEWKVAARIRERLGDSVHVNVTSYNRNVLLTGEAPNETV
                     RGQLDGIVAGVENVRGVINEVAIAPNSSLTARGNDTLITSNVKARMIDSGAVSAHNVK
                     VVTEANVVFLLGIVPRSEADAAAEVARTSQGVRKVVRVFEYISDEEARRLDNPTGARR
                     N"
     gene            complement(4183..4776)
                     /locus_tag="PKN25_01430"
     CDS             complement(4183..4776)
                     /locus_tag="PKN25_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNV89586.1"
                     /translation="MNLIDRISHQFEDNMRASLEALELLAAPIAGAVELITASLLDSG
                     KVLVCGSSGSAGDARRFAAQLVNGFELERPALAAMALSADSSSLAPIAGDPDTQALFA
                     RQIAAFGQPGDILLTLSANPDSARVLDAIRAAHERDMRVIALTGAGGGRTAETLGPAD
                     ILLCAPAERIARIREIHLLILHCLCDGIDCLLLGVED"
     gene            complement(4821..5273)
                     /locus_tag="PKN25_01435"
     CDS             complement(4821..5273)
                     /locus_tag="PKN25_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNV89587.1"
                     /translation="MGGRSGGWTRSAGEDARGAHAGADSIGARPTPAQARGAAAEALA
                     AEHLAAHGLRVIARNVRCRGGEVDLVCLDRSHVVFVEVRLRRNNRFGGAAESITAAKQ
                     RRVLIAAQWWLGGAGRRFRDAACRFDAVLLDALDPARIIWLPGAFDAG"
     gene            5272..6159
                     /gene="rsmI"
                     /locus_tag="PKN25_01440"
     CDS             5272..6159
                     /gene="rsmI"
                     /locus_tag="PKN25_01440"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNV89588.1"
                     /translation="MTVSAPLPDRPLSSTPSLYVVATPLGNLHDITLRALAVLAAVDV
                     VAAEDTRHSQRLLDAHGIRARMLAAHQHNEHEAAGVIVAELAAGRQVALITDAGTPAV
                     SDPGARLVARVRAAGHPVVPLPGPCAAVAALSVSGFAEGGFRFVGFLPAKAGARAAAL
                     RELADERAAMVFHEAPHRIVECVADMEQVLGGARELLIAREMTKLHEQIVRMPLAQTA
                     AWFAADTNRLRGEFVLVVEGAPAGEVVDARARRVLDLLLAELPVKGAARLAAEITGAP
                     RKALYALALERKGEDSPQA"
     gene            6234..7274
                     /gene="pyrC"
                     /locus_tag="PKN25_01445"
     CDS             6234..7274
                     /gene="pyrC"
                     /locus_tag="PKN25_01445"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNV89589.1"
                     /translation="MQSITLIRPDDWHLHVRDDAALDAVVPHTAARFGRALIMPNLKP
                     PVTTTAQALAYRERILAAARGSRFEPLMSLYLTDNLSPDEIDRARASGHVVACKLYPA
                     GATTNSDAGVTAIDKIYAVLERMEKLGMVLCVHGEATGAEVDVFDRERVFVERTLSPL
                     VRRFPGLKVVFEHITTAEAAQFVRAAGSHVAATVTAHHLLLNRNAIFAGGIRPHHYCL
                     PVLKRESHREALIAAVTSGNARFFLGTDSAPHARSTKENACGCAGCYTAHAGIELYAE
                     VFDAAGALDRLEAFASLNGPAFYGLAPSSDTITLRREPWSVPASYPYLGEDPLVPLRA
                     GEQVGWKLVEGA"
     gene            7324..7779
                     /locus_tag="PKN25_01450"
     CDS             7324..7779
                     /locus_tag="PKN25_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89590.1"
                     /translation="MSIRPGVRGGAGRALRRGLAAVGLLAVLAGCENSATAYMIDGNQ
                     HALILVREQKFFWDDELRQAMVVSRLPACQKRIRIHPGSATLVEMKIYAAGDGLWALH
                     QGARWYLAGTEECRVQDWDNPGDRPPGALVGSFVLKDGAPAFVPAPATK"
     gene            7820..8293
                     /gene="rnpB"
                     /locus_tag="PKN25_01455"
     ncRNA           7820..8293
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKN25_01455"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            8654..9097
                     /gene="mraZ"
                     /locus_tag="PKN25_01460"
     CDS             8654..9097
                     /gene="mraZ"
                     /locus_tag="PKN25_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNV89591.1"
                     /translation="MFQGAVALNLDAKGRLAIPARHRDALAVDNGQVVLTAHPHGCCL
                     VYPVPAWNPIRDHVLRAPSLDPQAAMLKRLLVGFAQEETLDAAGRVLVAPSLRKFAAL
                     EKQVWLVGQGAHFELWSDERWEKQQQAMLELVNTGLPPGFESLAL"
     gene            9094..10029
                     /gene="rsmH"
                     /locus_tag="PKN25_01465"
     CDS             9094..10029
                     /gene="rsmH"
                     /locus_tag="PKN25_01465"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNV89592.1"
                     /translation="MNLAHAHVSVLLSEAVDALAIRADGLYVDGTFGRGGHSRAVLQR
                     LGPQGRLIAFDRDPLAIAAGQAVGDARLTLVHSAFSALDDELARLGVQAVDGVLLDLG
                     VSSPQLDDAARGMSFRFDAPLDMRMDTSRGQTVAEWLAEASVGQITEVIREYGEERFA
                     HAIAKAIANARAGGAVATTGQLAALVEKAVRTREPGQHPATRTFQALRIFINQELEEL
                     SRVLPVCVSRLRPGGRLAVISFHSLEDRIVKRFMRDESRPPVLPRRLPVRAADLPAPR
                     LQLVGKAMRPSEAEVAANPRARSAVLRVAERTEAP"
     gene            10029..10295
                     /gene="ftsL"
                     /locus_tag="PKN25_01470"
     CDS             10029..10295
                     /gene="ftsL"
                     /locus_tag="PKN25_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNV89593.1"
                     /translation="MVRIDAVLVALAVASALGVVASQHQARRLHGEYEREQARAQALE
                     VEWGQLQLEQSTWATHGRIEKLARDRLHMQLPPEGQVLVVEPQS"
     gene            10292..12058
                     /locus_tag="PKN25_01475"
     CDS             10292..12058
                     /locus_tag="PKN25_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNV89594.1"
                     /translation="MKKQRAVTFNHNPLLKRELPAWRPRIIMLALMGCSLALVGRALY
                     LQGVNNDFLQAKGESRYARVLEVPATRGRITDRHGDILAVSTPVRAVWALPADARLEP
                     AQVRELARLLEMNVGELNARLAAGRDFVYLKRQVPPEVAQAVADLRLPGIHQEKEYRR
                     YYPGGEVMAHILGFTDLEDRGQEGIELAFDKRLSGRAGARRVIKDRRGQIVEDVEAIR
                     PPRDGDEVALSIDGKIQYLAWSALREAMEEHKPKAAAAVVLDVRTGEVLALVNAPTFN
                     PNNRANLTGAQLRNRVFTDAFEPGSVMKPFIAALALERGKVKLNTPIDTGNGRMTIGT
                     ATISDTKRHGVITMAQVVQKSSNIGTVKMAQQLGAEEMWHLFDQLGFGTPLNLGFPGE
                     TSGRLRPAKTWRPIEQATMSYGHGISTSLIQMARAYLVFAREGELVPLSLTRVDGTPS
                     SGRRVFSADTARQMRTVLAMAAGPGGTAIRAQVPGYTVAGKTGTAHKLEDGRYARKYI
                     SSFVGFAPASDPRIVVAVMVDEPSAGQHYGGTVAAPVFSRITEGTLRTLGVAPDMPLT
                     PLQLARRGGDEAASEGSSRESM"
     gene            12064..13560
                     /locus_tag="PKN25_01480"
     CDS             12064..13560
                     /locus_tag="PKN25_01480"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNV89595.1"
                     /translation="MQALAVLDRLRALGVHADDITADSRKAGPGCVFAAWPGQRTDGR
                     RFLADAVVHGAAALLWEDGDGFCAPALAVPGFGVRGLRELAGHLAHELHGRPSQSLWL
                     AGVTGTNGKTTVSQMLARALGELGERCGIVGTLGCGFPDALEEGLNTTPDALELQRWF
                     ARFRNQGAVAAAMEVSSIGLDQGRVNGAQFDVAIFTNLSRDHLDYHGTMEAYAAAKAR
                     LFERPELSACVINIDDPWGLALARRLVVEGRAVIACTLYAANADAVPGARVLRADRLQ
                     AAPAGLRFTLVWEGREAEIGVRMVAHFNVSNLIAVAGALLARGIAFDELPPVLGRLAP
                     PQGRMQLVGGVCEPLVVIDYAHSPDALAKVLEALRGTVHSRRGRLVCVFGCGGDRDPG
                     KRPMMGEIASAIADRVVITSDNPRSEDPQRIVEAIAAGAGAAAECVVDRAQAITRAVG
                     EARADDVVLIAGKGHEPYQEILGQRLPFSDLEQAKRALAAWHARENGR"
     gene            13557..14969
                     /gene="murF"
                     /locus_tag="PKN25_01485"
     CDS             13557..14969
                     /gene="murF"
                     /locus_tag="PKN25_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNV89596.1"
                     /translation="MMSLHDATRALACHGARATGEGRFDSVGTDTRALAPGQLFVALR
                     GERFDGHDFVADAAKAGAAAAMVDAAWSAGKADAGLPLLVVDDTRRALGTLAAAWRAR
                     FALPLIGVTGSNGKTTVKEMCAAILRVQASRDGFGEESVLATRGNLNNDIGMPLTLLE
                     LRDFHRAAVIEMGMNHPGEIGYLSALAQPTVALVNNAQRAHLQGMGSVDEVAREKGAI
                     YEGLGGAGVAVINADDPHAGYWRSLNAGRPIVTFGIDHPADVSARCTLRGLGSRLELD
                     IPQGKIDFALQVPGLHNVRNALGAAAACLAAGVPLEAVAEGLAGFAGTRGRLQRRAGP
                     RGALILDDSYNANPDSVRVGIDVLAAMPGHTWLVLGDMGEVGETSAQVHDEVGGYAKS
                     KGIDGLFALGEMSAVAAHNFGEGARHFTSVEALVKALVPRLDADSVVLVKGSRFMRME
                     RVADALAAVHNPAPSTETGS"
     gene            14971..16059
                     /gene="mraY"
                     /locus_tag="PKN25_01490"
     CDS             14971..16059
                     /gene="mraY"
                     /locus_tag="PKN25_01490"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNV89597.1"
                     /translation="MLLELALWLGQDIRGFNVFGYITLRTVLAALTALAISLIAGPAV
                     IRWLAAKKIGQAVRDDGPKSHLTKAGTPTMGGALILIAIGVTILLWGDLKNAYVWVTL
                     LVTLGFGAVGWVDDWRKVVHRDPKGLASRWKYLWTSAIALAAAIYLGVTADTPAETEL
                     IVPFFKAIAYPLGIFGFIALTYFVINGTSHAVNLTDGLDGLAIMPTVMVAAALAIFAY
                     VAGHVGFSKYLGVPYVAGAGELAVFCGAICGAGLGFLWFNAYPAEVFMGDVGALALGA
                     ALGTIAVIVRQEIVLFIMGGLFVAETLSVMVQVLYFKATGGKRIFRMAPLHHHYELGG
                     WKETQVVVRFWIITIMLVLFGLSTLKLR"
     gene            16056..17456
                     /gene="murD"
                     /locus_tag="PKN25_01495"
     CDS             16056..17456
                     /gene="murD"
                     /locus_tag="PKN25_01495"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNV89598.1"
                     /translation="MSALTGKHVLVLGLGESGLAMARWCALRGARVRVADSRAVPPGV
                     DALKADAPLAEIFMGSFGDEVLDGVDLVALSPGLDPRVGVAAQARRRGLPLTGEMSLL
                     AQALDELGVRAQTRILAITGTNGKTTTTALTAALAQSVGIDAVAAGNISPAALDVLME
                     RLEIGAALPACWVLELSSFQIETMRGLDAEAATVLNVTDDHLDRYADLEEYAATKAAI
                     FQGEGVQVLAREDARVAAMALPGRRTIRFGTAAPASDTDYGLADLDGRAWLVRGGERL
                     LALADLPLAGRHNAANALAALALCEGGLGIAPQILIPGLKAFRGLPHRVELVAERADG
                     VRFYDDSKGTNVGATVAALAGMDCPVVLIAGGDGKGQDFSPLAPVLAGKARALLLIGR
                     DAGRIEQAVAGCGVAIERVPDLDAAVARANAWAQAGDAVLLSPACASLDMFRNYAHRA
                     EVFVAAVRRLPGVKPR"
     gene            17453..18682
                     /gene="ftsW"
                     /locus_tag="PKN25_01500"
     CDS             17453..18682
                     /gene="ftsW"
                     /locus_tag="PKN25_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNV89599.1"
                     /translation="MKFASTLTGLLQREGGAADPARAASGRLGRQGAPARELDLLLIW
                     AAVGLLLLGLVMVYSASIAIAEGSRFTAYQSHYFLLRHAVFLAVGIGLGLMAFQLPMA
                     RWQQLAPALFVAGVVLLVVVLIPGIGREVNGAQRWLSLGPVNLQPSELMKIFAALYAA
                     DYTVRKLDVMGSFVKGFVPMMAVILLVGFLLLREPDFGAFVVITTIAFGVLFLGGVNV
                     RVFVLLAVVAVIGFVILIWTSPYRRDRIFGFMDPWQDAYGKGYQLSHSLIAFGRGEWF
                     GVGLGGSVEKLFYLPEAHTDFLLAVIAEELGFAGVLTVVALFALVVHRAFIIGREAIK
                     LERYFAGLVAQGIGLWIGIQSFINMGVNMGLLPTKGLTLPLMSFGGSGIVANCVALAI
                     LLRVDWENRQLLRGGRA"
     gene            18679..19749
                     /gene="murG"
                     /locus_tag="PKN25_01505"
     CDS             18679..19749
                     /gene="murG"
                     /locus_tag="PKN25_01505"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNV89600.1"
                     /translation="MKTLMVMAGGTGGHIFPGIAVAEELRARGWRIVWMGNPEGMEAR
                     IVPPRGYDTAWVRFGALRGKGLLRKLLLPVTLLSGFWQALREIRRVRPDVVLGMGGYI
                     TFPGGMMAALLGRPLVLHEQNSVAGLANRVLARVADRVLSGFPEVLEQAEWMGNPVRA
                     EIAAVLPPAERMAGRSGPLRVLVVGGSLGAAVLNETVPQALLRLPAEQRPVVVHQAGE
                     RQIEALRAAYARAQVDGELRPFIDDMAAAYADADLVICRAGALTVAELAAAGVASLLV
                     PFPYAVDDHQTGNARFLADRGGAYLLPQSELTPDRLAGILSSLDRGRLLHMAENARAL
                     AKPLAAVAVAHVCAELGGGEQA"
     gene            19746..21161
                     /gene="murC"
                     /locus_tag="PKN25_01510"
     CDS             19746..21161
                     /gene="murC"
                     /locus_tag="PKN25_01510"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNV89601.1"
                     /translation="MKHKVRNIHFVGIGGAGMSGIAEVLANQGYRVSGSDLGDNAATR
                     RLHKMGAHVVRGHDAANVADADVVVVSTAVKADNPEVVAARERHIPVVPRAQMLAELM
                     RFKHGIAIAGTHGKTTTTSLVASILAEGGIDPTFVIGGRLNAAGANARLGTGDYLVAE
                     ADESDASFLLLNPVISVVTNIDADHMDTYGHDFGRVKQAFVDFLHRLPFYGVAVLCED
                     DPNVREIIPQVAKQIVRYGFSETANIRAENVRAEGGRMRFDCVRVNGTTSRLSIELNL
                     PGLHNVLNALAAIAVATEVQVPDAAIVKALAEFNGVGRRFQRYGEVALHDQDGDEAGR
                     FTLVDDYGHHPVEMAATIAAARGAFPGRRLVLAFQPHRYTRTRDCFEDFVKVLSTVDA
                     LLLAEVYAAGETPIVAADGRSLARAIRVAGTVEPVFVEEIADMPRMILSAARDGDVVI
                     TMGAGSIGAVPGKLASIEEAA"
     gene            21158..22072
                     /locus_tag="PKN25_01515"
     CDS             21158..22072
                     /locus_tag="PKN25_01515"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNV89602.1"
                     /translation="MKARFGKVAVLFGGSSAEREVSLMSGAAVLAALQAAGVDAHAFD
                     PAERDLHVLKEEGFERAFIALHGRGGEDGTVQGALELMGIPYTGSGVMASALSMDKWR
                     TKMVWLSAGLPTPRYAILEHDSDWDAVVAELGLPIFVKPVHEGSSMGATKVVEAGQLR
                     AAWELAARYDRLVIAEEFICGAELTAPFLEDRALPLVRIVAPGGNYDYQHKYFTDDTR
                     YDCPSGLPAAQEAELQAMVMKCARVLGCRGWGRADLILTEDGRPYLLEMNTSPGMTGH
                     SLVPMSARVAGLDFTALCLEILQGARLG"
     gene            22065..22928
                     /locus_tag="PKN25_01520"
     CDS             22065..22928
                     /locus_tag="PKN25_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HNV89603.1"
                     /translation="MAEAGIRPASIRSARRPAAGGAARAGAERGLWHRPALLNLISDL
                     LTLGAAVALGWALVIWFVSRPLFPLRELVVLTDPGQVTVEQLDYAARLAVQGNFFTVD
                     LDGVKETFEKLPWVRKAEVRRRWPDALELRLEEHEAVAYWTVSESGEARLVNRQGEVF
                     VAASNADMPQFDGPQGSAGWLLARHAEFSSMLQPLGVRLVGLALSAREAWQLQLDNGM
                     TIVLGREQDKSPLMERLRRFIAVWPRVHEQIDIDIKTADLRYAGGFALTPADASVLHQ
                     PVAPAARKGRK"
     gene            22925..24154
                     /gene="ftsA"
                     /locus_tag="PKN25_01525"
     CDS             22925..24154
                     /gene="ftsA"
                     /locus_tag="PKN25_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNV89604.1"
                     /translation="MSKQYNELIVGLDIGTAKVTCMVAEVKPDGRLNVVGLGTQSTNG
                     LKRGVVVNIEATVDAISRVVQEVELMADCKIHEVYTGLAGSHIKSFNSSGTVAIKEKE
                     VSPLDVERVIEVARAMPIPAEQQILHILTQEFIIDGQGGVREPIGMSGVRLEVKVHIV
                     TGAVSAAQNVIKCVRRCGLEVMDLSLQPLASSHAVLTEDEKELGVCMVDIGGGTTDIA
                     VFTQGAIRHTAVIPVAGDQITNDIAMALRTPTAEAEEIKIRHGVAMHHMADPEEMIEV
                     PGVGDRPPRKLSRQALADVIEPRVSELFELVQAELRRSGYEELLSSGIVLTGGSAVMR
                     GMAELAEDVFHMPARVGAPQYDGGLADVVCQPRYATAMGLVMEGAVQRRRGLQARDTR
                     SVKQFFGRMKAWFERNF"
     gene            24296..25438
                     /gene="ftsZ"
                     /locus_tag="PKN25_01530"
     CDS             24296..25438
                     /gene="ftsZ"
                     /locus_tag="PKN25_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNV89605.1"
                     /translation="MFEIVEKEATGTVIKVVGVGGAGGNAVDHMIREGVKGVHFISAN
                     TDAQALKRCLAPVKVQLGITGLGAGSKPEAGRAAAQESRDAISAALEGAHMVFITGGM
                     GGGTGTGAAPVVAEIAKEMGLLTVAVVTKPFDFENRIRVAESGIEELTRHVDSLIVVL
                     NDKLLEVFGDDAGFEECFRSADNVLRSAVGGIAEIINVPGLVNVDFQDVRTAMAEMGR
                     AMMGSAEAAGMDRARIAAEQAAVSPLLEGTELSGARCVLINITASKSLKMSEVRDAVK
                     TVQAFAAPEAFVKYGTVFDDTMEDRIRITVVATGLGAPRAARQPVMQVVQGTGTYGPA
                     MGGAVDVTNLDVPAVMRSGRRTTVEAMSTSGVGTYDIPAFLRRQAD"
     gene            25647..26570
                     /gene="lpxC"
                     /locus_tag="PKN25_01535"
     CDS             25647..26570
                     /gene="lpxC"
                     /locus_tag="PKN25_01535"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNV89606.1"
                     /translation="MIRQRTLKSVVTATGVGLHGGRKVTLTLRPAAPDTGIVFHRVDL
                     DPPLTLPADPYAVCDTRMCSGLEQGGHKVGTVEHLMSALAGLGIDNLHVDVDAPEIPI
                     LDGSSAPFVFLLQSAGIEEQRAAKKFLRVKKAVEYVEGDKWVRLEPYDGFRLAFSIVF
                     NHPAIDSTSTRVEVDFARDSYIRDVARARTFGFMQDVEFMQANGLALGGSLENAIVMD
                     EYRVLNAEGLRYADEFVKHKVLDAIGDLYLCGHPLLASYSAHKAGHALNNQILRVLLE
                     DKEAWELVSFDQEAATPPAVSHQFAPLVALA"
     gene            26695..26883
                     /locus_tag="PKN25_01540"
     CDS             26695..26883
                     /locus_tag="PKN25_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89607.1"
                     /translation="MLIMRLLLLLAVLSIGGSAILWLLTGNPRYKAWAWKALRATLVM
                     LFVLFALFAIERVLAPVA"
     gene            complement(27073..27495)
                     /locus_tag="PKN25_01545"
     CDS             complement(27073..27495)
                     /locus_tag="PKN25_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HNV89608.1"
                     /translation="MTRLLSRFLGGDALARLQDHAARLNRLQAVLDGMLPPQLQGQCR
                     VANLKEGSLVVAARGGAAAVRVRQILPSLLERLQHGGHPVQSIKVKVGTPEQTAPERH
                     APQRQISEAAKAELHDFADSLPVDSPLRASIERLIRRA"
     gene            27580..30309
                     /gene="secA"
                     /locus_tag="PKN25_01550"
     CDS             27580..30309
                     /gene="secA"
                     /locus_tag="PKN25_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNV89609.1"
                     /translation="MISGLLKKIFGSRNDRLVRQYMHTVRKINAFEAEISALSDEALR
                     GKTAEFRQRVADGESLDALLPEAFAVVREAGKRVHGMRHFDVQLVGGMVLHHGKIAEM
                     RTGEGKTLVATLPAYLNALSGKGVHVITVNDYLASRDAEWMGRIYGFLGLTTGCNLSR
                     MSHDQKQAAYAADITYGTNNEFGFDYLRDNMVYAVGERVQRALAFAIVDEVDSILIDE
                     ARTPLIISGQAEDHTDLYLKMNQVAPMLKRQEGGLDDKDEVIEAGDYTVDLKAHQVLL
                     TEAGHETAEQILVRMGLLAEGGGLYDPGNILLVHHLYAALRAHALYHRDQQYVVQNGE
                     VVIVDEFTGRLMPGRRWSDGLHQAVEAKEGVRIQAENQTLASITFQNYFRMYGKLAGM
                     TGTADTEAFEFQQIYGLETVVIPTNRPTRRRDENDKVYRTASEKWNAVIEDIRACVER
                     GQPVLVGTTSIETNEFLSGVLKKAKIEHQVLNAKQHDSEAQIVAQAGRPGMVTIATNM
                     AGRGTDIVLGGNIEKPVAAVRDDATLDPVQRETRIAALREAWQKVHDEVIAAGGLHII
                     GTERHESRRIDNQLRGRSGRQGDPGSSRFFLSLEDPLMKIFAGERLNAIMVRLKMPEG
                     EAIEHAMVTRSLESAQRKVEQRNFDIRKQLLEYDDVANDQRKVIYQQRNELLESEDIS
                     DTVRAMRQGVLHDIFRRYVPVDSVEEQWELPALEQALLAECQLKLAVAAWVTAEPSLD
                     DEAILERVVQAGEEAYAAKTAMVDPAAWHQFERNVMLQSLDTHWREHLAALDHLRQGI
                     HLRGYAQKNPKQEYKREAFELFESLLDAVRTDVTKILMTVQIRTEAQLEEAETPQQVE
                     NVQYQHADYDEALGGGAEQAGAQPATAGPKVGRNDPCPCGSGKKYKHCHGKLN"
     gene            30332..31570
                     /gene="argJ"
                     /locus_tag="PKN25_01555"
     CDS             30332..31570
                     /gene="argJ"
                     /locus_tag="PKN25_01555"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNV89610.1"
                     /translation="MAVNLATPNPAELLPVKGVRLGVTEAGIRKANRRDLTVIELSEG
                     SRVAGVFTQNRFCAAPVQVCRQHLTGGAIRALLINTGVANAGTGEPGLANANACCAAL
                     ARELGIEAGQVLPFSTGVILEPLPVDRLVAGLPGAVADLRADGWFDAAHAIMTTDTLA
                     KAVSRQVRIGERTVTLTGISKGAGMIRPNMATMLGFLACDAAIAQDLLDDLVREAADL
                     SFNSITVDGDTSTNDSFILIATGAAGNAEIADADSEDYRALRAAVIEVAVGLAQAIVR
                     DGEGATKFMTIAVEGGRDREECRKVGYAVAHSPLVKTAFFASDPNLGRILAAIGYAGI
                     DDLDVAKLRVWLGNPEESVLVAEHGGRAASYLEEAGSRIMKHAELTVRIALARGDAAA
                     TVWTCDFSYDYVKINADYRS"
     gene            31779..33833
                     /locus_tag="PKN25_01560"
     CDS             31779..33833
                     /locus_tag="PKN25_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV89611.1"
                     /translation="MQDPYTSRDPRTRGLASRAGPLVLLGLLALDARAEWGDPDPFFS
                     EIPMVLTASRLAQSPLDAPAPVTVIDREMIVASGFNEIHDLLRLAPGFLVADHPDGPP
                     TVATHGLGDAHARRIKVMVDGRTVNSPLWGNVIWDNLPLRVDDVERIEVVRGPNGAAY
                     GVNAFQGVVNIITRAPSTESGRAFVVRTGKNGFRDYGVRLNGAGGSAFDWRLSASRRE
                     LTTFEPHFRERLGDWHSSESLTRNTAHFSAVLQLSAAEELGLQVGVSEGDSKRGAQAY
                     LDYPPHTEEDRALHLQFGWRRHIDAESVLSVQYFHQGERAGGSWPVIQAQGTTTASFP
                     TSRASEARRHDLELQYDTRLGRRLTGMLGIGLRSEHARSLHLFGTTRWLDSTHGQVFG
                     SLTWNPLDPLKIDLGGTLEHHDYSGRLFSPRLAFNYALDEDSALRLSTGTAYRAPSLM
                     ESRAFQTDREGDTIRRIGFRVFDPLQSERMRFVDLGYVAQLRSFGLGFDARVFREYYR
                     RFIDDKSCVFGECPRPEPADYVPIDPGKKSFYFLNVDDFRMTGAEFTLDWRKPGWGRA
                     VLSQAFIRIDHGEPYSDRDIPNSAPHATTSLLLIKDFPGRWQASLGYYHNGLYYWMNG
                     GDIVPRTDRFDLRVARRFGPPGSDNEVSLTALSLKGRYADFHEDKFRHEPDLYLGVRI
                     GW"
     gene            33902..34792
                     /locus_tag="PKN25_01565"
     CDS             33902..34792
                     /locus_tag="PKN25_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNV89612.1"
                     /translation="MLALLPVLGLSVAHAMEVAVLVSEPGGRGQRFVEALDAALAPGV
                     HRLAGAGLAGGEADTALLGRADLVLAVGHAAAERALRDSSRPVLAVLVGLRDLDRLKR
                     AHPARDLGAVVLDQPLERHLRLVRAVLPDAKRVGVLLGPDSVVQLPGLQHAADALRLQ
                     ADVHEVREVADLVPALDHLLFAGDALLPLPDGLVSGPAAARTILLTSYRHRRPVFAHS
                     AAYVTAGALAAVFSTPEHVAVDVADLLAAAKEPAALVRKIHSPRRFDVAVNRAVARAL
                     GLVVADEQVLRERIADGGAQ"
     gene            34789..36123
                     /locus_tag="PKN25_01570"
     CDS             34789..36123
                     /locus_tag="PKN25_01570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV89613.1"
                     /translation="MIAALSRYSLRRQMLLVLGLPSVVFVLCIAALFAYQSSRALDQA
                     LLERGRAMVRFLAPAAEYVLVSGNREALGALMARVQVQPDVVGVGFYDPGGDALATLG
                     EGLSPRLAAPSRAEGDEIRVDLADGAIGFEAPVVAVPATVDDYAEDSSSKTAPVVGQV
                     RVVLGTARLDAEKRGVFLTVSLLVLGVLATAMAIAVRLAGSVGEPVAALVDAVGRMAD
                     GDLAARVPARARNGELKALEAGFNAMAEAIAENQRTLQARIDEATVQLAWQARHDPLT
                     GLANRRAFEERIEQAVRAHRRSGDQITLFYIDLDHFKVVNDSCGHAAGDRLLSELGAL
                     LKGRLRSEDEVFRIGGDEFAAILRGCGREDARRIAEGLCAAIAAHAFESDGRAFRVGA
                     SIGLARMDPDLQEADALMIAADQACYAAKHGGRGHVVEYPLPFVAPGSARPG"
     gene            complement(36162..36545)
                     /gene="apaG"
                     /locus_tag="PKN25_01575"
     CDS             complement(36162..36545)
                     /gene="apaG"
                     /locus_tag="PKN25_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNV89614.1"
                     /translation="MSETTPYRVQVTAVAEYVADQSRPQDEHFVFAYHITVLNTGEVR
                     AQLLARHWVITDGNGKMQEVHGQGVVGEQPVLGPGQAFRYTSGCVLATPVGTMQGSYR
                     MRADDGHEFEAEIPAFMLAVPKALH"
     gene            36614..37990
                     /gene="purB"
                     /locus_tag="PKN25_01580"
     CDS             36614..37990
                     /gene="purB"
                     /locus_tag="PKN25_01580"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNV89615.1"
                     /translation="MSNAAPSPLTALSPLDGRYHGKVAGLREHFSEHGLIRNRVRVEV
                     EWLKALAAEPALAEIAPFSPATVVELDAVVAQFSTDDGEAVKAIEATTNHDVKAMEYW
                     LKKRLGHNAEVMKVSEFIHFACTSEDINNTSHALMLAEGRDRVLLPALDAVIARFREL
                     AHALADLPMLSRTHGQPASPTTLGKEMANIAARLMRARAAIAGVAMTAKFNGAVGNYN
                     AHLSAWPQFDWEGFNRRFIESLGLTFNEYTIQIEPHDAMAELFDAIARANTMLIDACR
                     DIWMYISLGYFKQKLKEGEVGSSTMPHKVNPIDFENAEGNFGLANAVLKHFSEKLPIS
                     RMQRDLTDSTVLRNMGVGFGHTVLALDSCLRGLGKLEAEPTRLAADLDDCWEVLAEPV
                     QTVMRRYGIANPYEQLKAMTRGKGITREALQEFIRTLAIPAEARDHLLAMTPASYVGK
                     AAELARRI"
     gene            38075..38392
                     /locus_tag="PKN25_01585"
     CDS             38075..38392
                     /locus_tag="PKN25_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HNV89616.1"
                     /translation="MAFADNLKTLPGVSHLAALNLIDAADAVVATIENKPGQAGSLAV
                     YNHLAQLYGAISPEAAKKGLELYAEHTEDARANPGKHPNIDRLIGLIERGETLRVKQV
                     FAV"
     gene            complement(38705..39637)
                     /gene="secF"
                     /locus_tag="PKN25_01590"
     CDS             complement(38705..39637)
                     /gene="secF"
                     /locus_tag="PKN25_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNV89617.1"
                     /translation="MEFFRIKKDIPFMRHALVFNVISLLTFALAVFFLLTRGLHLSVE
                     FTGGTLVEVSYAQAPQLEPIREALAGSGYPDAQVQNFGSARDLLIRLPNREDLETSRV
                     SETVMNTLQQVGGPAPELRRVEFVGPQVGKELAADGSMALLLVIVGIMIYLALRFEWR
                     FAVSAIIANLHDVIIILGFFAFFQWEFSLPVLAAVLAVLGYSVNESVVVFDRVRETFK
                     KKRNMTTPQVLDHAITSTISRTVITHGSTQVMVLSMLVFGGETLYYFSLALTIGICFG
                     IYSSVLVASPLVMWLGVSREQFIKPKKEKEEAVV"
     gene            complement(39665..41530)
                     /gene="secD"
                     /locus_tag="PKN25_01595"
     CDS             complement(39665..41530)
                     /gene="secD"
                     /locus_tag="PKN25_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNV89618.1"
                     /translation="MNRYPLWKNLLIGLVLLWGLIYTLPNFYGEVPAVQVSSAKATLK
                     LDTAATTDRIAGALKAAGIENTGIFSDATSVRARFKDTETQLRAKDVIEQTFNPDAAD
                     PSYVVALNLLSASPSWLTSIQALPMYLGLDLRGGVHFLLEVDMPGALVKRLDSTTGDL
                     RTLMRDKSVRHAGITREGNTVVIRFRDAAQREAGRRAILDSTTDLQLVERDDAADDLK
                     LVATLTPQAQQTMRDFAIKQNITTLHNRINELGVAEPVIQQQGAERIVVQLPGVQDVA
                     KAKDILGRTATLEVRMVDDTPGAIEQALAGSVPFGTELYNERGGSPLLVKKQVVLTGD
                     RLTDAQPGFDGQTNEPAVHLTLDAAGARIFRDVTRESVGKRMAILLFEKGKGEVVTAP
                     VIRSEIGGGRVQISGRMSTVEANDVALLLRAGSLAAPMEIIEERTVGPSLGAENIAKG
                     FNSTLWGFLAIVVFMCGYYLLVGLVSSIALAANLLLLVALLSLLQATLTLPGIAAMAL
                     TLGMAIDSNVLINERIREELRNGASPQAAIAAGYDRAWDTILDSNITTLIAGIALLVF
                     GSGPVRGFAVVHCLGILTSMFSAILVSRMLINFIYGRRRKVESLSIGQVWKPDTK"
     gene            complement(41691..42017)
                     /gene="yajC"
                     /locus_tag="PKN25_01600"
     CDS             complement(41691..42017)
                     /gene="yajC"
                     /locus_tag="PKN25_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNV89619.1"
                     /translation="MLISNAYAQAAGAADPTGGLMGLLPLILMFVVLWFLMIRPQMKR
                     AKEHKAMVEALAKGDEVVTGGGIAGRVVEVGDSFVQIEVAPNTVVAAQKQAVATVLPK
                     GTLKAI"
     gene            complement(42109..43221)
                     /gene="tgt"
                     /locus_tag="PKN25_01605"
     CDS             complement(42109..43221)
                     /gene="tgt"
                     /locus_tag="PKN25_01605"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNV89620.1"
                     /translation="MQFELLTTDGGARRGRLTLAHGVVETPVFMPVGTYGTVKAMTPA
                     MLSDIGAQICLGNTFHLWLRPGLDIISAHQGLHRFMAWDKPILTDSGGFQVFSLGALR
                     KISEEGVKFASPIDGAKLFLTPEISMQIQSVLNSDIVMIFDECTPYPATRDEAAKSMR
                     LSQRWAKRSRDEFDRLENKNALFGIVQGGMYEDLRDESQAALEDIGFHGFAIGGLSVG
                     EPKDDMARILAHTAPRLPQGKPRYLMGVGTPEDIVAGVAAGIDMFDCVMPTRNARNGW
                     LFTRYGDIKIKNAVHKADTRPLDPSCDCYTCRNFSRAYLHHLHRTGEILGSMLNTVHN
                     LRYYQTLTAELRAAIAAGEFAPYVQRFRGERATGTG"
     gene            complement(43211..44287)
                     /gene="queA"
                     /locus_tag="PKN25_01610"
     CDS             complement(43211..44287)
                     /gene="queA"
                     /locus_tag="PKN25_01610"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNV89621.1"
                     /translation="MSLTLADFDYTLPPELIAQAPLAERSASRLLVLEPQEGAAPRLT
                     DAVFSALAERVNPGDLLVFNDTRVIHARLHGVKDSGGQVEVLIERPVGPHEALAQVRA
                     SKSPKTGSRLRLADAFEVEVLGRVGEFFHLRFPPDEDLLALLERHGKLPLPPYIQRAA
                     GEADESRYQTVYARAPGSVAAPTAGLHFDDALLARIAARGAKCAWLTLHVGAGTFQPV
                     RVDDLGEHRMHRERYVIPQETVDAIAATRAAGGRVIAVGTTSMRALEAAAQEGALEAG
                     SGETEIFILPGFRFQVVDALVTNFHLPRSTLMMLVSAFAGMDSIRRAYAHAVEQRYRF
                     FSYGDAMFITRRNESENDKNDDAV"
     gene            44380..44464
                     /locus_tag="PKN25_01615"
     tRNA            44380..44464
                     /locus_tag="PKN25_01615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44414..44416,aa:Leu,seq:caa)
     gene            complement(44690..45586)
                     /gene="nhaR"
                     /locus_tag="PKN25_01620"
     CDS             complement(44690..45586)
                     /gene="nhaR"
                     /locus_tag="PKN25_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HNV89622.1"
                     /translation="MLNYKQLHHFWTVARAGGIVRAAERSGLAPQTLSGQIATLETDL
                     GVNLFKRQGRGLVLTETGRMVLDYAEEIFRLGNELEEALQSRATGRAVPFRVGIADVV
                     PKAIAWLLLAPSMDLPEPMRIVCREGKFDALLGELAIHKLDVVLADSPLPPAMDVRGF
                     SHALGESTIGFYAAPALAARLAGDFPARLDGAPLLLPGVEASVRGPLLRWIEAAGLRP
                     RIVGEFDDSALMRAFAEAGAGIFPSASLVGEQLCRQGGLVHLGDAKGVTETYYAISVE
                     RRLTHPAVRAISEGAHAKQNFA"
     gene            45737..46069
                     /locus_tag="PKN25_01625"
     CDS             45737..46069
                     /locus_tag="PKN25_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNV89623.1"
                     /translation="MRIDLHCDGVETAPGLRDYVAQRMRASIGRFRDHIQWARVKVAD
                     VNGAEGGADKRCVVQLRLRNLPDVVFAITQLDVRAAVDEAADRVARVLAQRVRRHQRG
                     PAMAAIPA"
     gene            46163..46828
                     /locus_tag="PKN25_01630"
     CDS             46163..46828
                     /locus_tag="PKN25_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNV89624.1"
                     /translation="MENRISTLGRAEASVLQTNKVIRNTYMLLSLTLAFSALTAGLSM
                     AMGAPRLGILITLAGYFGLLFATTKFRDSSLGIVFVFALTGFMGFTLGPIISSYLALP
                     NGGSIVMQAMAGTAAIFLGLSAYAVTTKKDFSFMGGFLMVGILVAFLAGLGAIFFEIP
                     ALSLTVSAAFVMLMSGLILYETSNIIHGGETNYIMATVSLFVSIFNLFTSLLHLLGFA
                     NND"
     gene            46997..47962
                     /locus_tag="PKN25_01635"
     CDS             46997..47962
                     /locus_tag="PKN25_01635"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNV89625.1"
                     /translation="MKTTFLDFETAISELEEKIDQLRFVQDDPAVDISDEIKRLEAKS
                     QSLTKDLYAKLSPWQIAQVARHPQRPYTLDYASLMFTDFHELHGDRNYADDKAIVGGL
                     ARFNGQACVVIGHQKGRDTKEKIARNFGMPRPEGYRKAMRLMKLAEKFALPVFTFVDT
                     PGAYPGIGAEERGQSEAIGHSIYLMSELKTPIISTIIGEGGSGGALAIAVADRVLMLQ
                     YSTYSVISPEGCASILWKSAEKAADAAETMGITAARLKSLELVDKVVDEPIGGAHRDH
                     RAMAATLKRALADALRELEALSPSELVEQRYQKLMSYGRYKEKAA"
     gene            48027..49382
                     /gene="tilS"
                     /locus_tag="PKN25_01640"
     CDS             48027..49382
                     /gene="tilS"
                     /locus_tag="PKN25_01640"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNV89626.1"
                     /translation="MRTPPADVLPESAAAVLAAAGVGAGSRVCCALSGGVDSVVLFEL
                     LRRLQPRFGFSLRAAHVDHGLSPNAAAWARACAERCLAAGVPLQVFRVEVERAHADGL
                     EAAARAARHAALAQVDCDWLAFGHHQDDQAETLLFRLLRGTGVRGAAAMRAVEPGGAG
                     RAGRLRPLLGLRRAQLLEWAGAHGLGWVEDESNAELRFTRNALRHRLLPVAEGLFPAA
                     VPNLARAAAHFAEADALLGELAAIDAAACGAPVLCRADLLALTPARQANLLRWLLLGS
                     GAPLPGAAPLGEALRQLRACDPAHPLRLPLGAWVCCAYRDRVWLEPAQPPAPQALHWQ
                     GEAAMHWGGDEVRLVASIGAGLGRGCLEDAGEVLLGPRREGLRMRLHPARPRRELRKL
                     CQEAAIPAWLRDRLPVLWVDGEPAWVGGIGVAAEFACAPGAAGLVPEWRPGGVTPAPP
                     P"
     gene            complement(49406..50011)
                     /locus_tag="PKN25_01645"
     CDS             complement(49406..50011)
                     /locus_tag="PKN25_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV89627.1"
                     /translation="MNPACTHIIDDDEAIRDALEWLFKTRGVPCRSWASAEAFLADWR
                     GDWRGCIVLDIRMQGMSGLECFDELLARGCQLPVIFITGHGDVPMAVAALKKGAFHFI
                     EKPFNDHDLVDLVEKALATDDERLRAAASRETIEARLATLTQREREVMELILEGKYNK
                     VIADDLSISMRTVEAHRSRIFEKMEVRSAVELAQLLTTLRT"
     gene            complement(50008..52002)
                     /locus_tag="PKN25_01650"
     CDS             complement(50008..52002)
                     /locus_tag="PKN25_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV89628.1"
                     /translation="MSLSDPLPPPARRRWLQQLPYLSLALFLAAIAALVWLTREYDDE
                     AQRATLISDVLWMEQNLRFNLDRNENHLQEIGPELLATPTLSAQTEARLASLLTSERG
                     LARILWLSPEGEVRGAMPPLAPAQAVHDASADAAAVPVDESSVRLARAIGRPVYGPAH
                     PAPGGTHHFEVHVPVWSDEAQVGMVVGVYALSDVVMRELPWWFSERYHVAVLDSDGRT
                     IAAKSKVAPVSPRLSYSMPFDPPGHGLTLQISAYRSDPRWIPVLLGASIVLLAGIIVW
                     SVMQLRRQLARRQQAEVALRAESVFRKAMEDSMLTGMRARDLEGRLTYVNSAFCRMTG
                     FSADELLGRKPPMPYWDPERLEQTFELHRQIMTSGSASEGTEVRLRRKNGETLDVLVF
                     EAPLIDASGRHAGWMGSVLDITEQKRAREQARQQEERLQQSSRLITMGEMASTLAHEL
                     NQPLAAIASYTTGCINRLQDEAPLDRSELLDVHQRIARQAQRAGEIIRRVHDFVRRSE
                     PKREALDLAAVIRDAIGLIEADARKRGMRIRSELADGLPQVPADAVMIEQIVVNLVRN
                     AMDSMRDTPPAERVVAVHTAREGRFVTVTVADRGAGIPAETAARLFEPFFTTKQEGMG
                     MGLNICRSIAELHRGRLAFESRPGGGTIFTLSLPVDAEFE"
BASE COUNT         7664 a        17966 c        18421 g         8117 t
ORIGIN      
        1 ccgcgcccgc cgccgccgcc ggcggccttg atgatcaccg ggtagccgac cgcgcgtgcg
       61 atcttgacga tctccttcgg atcctcgggc agtgcgccgt ccgaacccgg cacgcagggc
      121 acgccggcgg ccttcatcgc atccttggca gagaccttgt cgcccatcag gcggatggtc
      181 tcggggcgtg ggccgatgaa gacgaagccc gactgctcga cgcgctcgcc gaagtcggcg
      241 ttctcggaga ggaagccgta gccggggtgg atggcctcgg cgtcggtgac ctcggcggcg
      301 gagatgatgg ccggcacgtt gaggtagctc tgcgcagagg gcggcgggcc gatgcaaacg
      361 gactcgtcgg ccagtcgcac gtacttggcg tgggtgtcgg cctcggagtg gaccgcgacc
      421 gtcttgatgc cgagttcgcg gcaggcgcgc aggatgcgca gggcgatttc cccgcggttg
      481 gcgatcagga tcttctcgaa catggcggac tcagccgatc acgaacaggg gctggccgta
      541 ttcgacgggc tcaccgttct cgaccaggat ggccttgacg acgccgctgg cgtcggcctc
      601 gatctcgttc atcagcttca tcgcctcgat gatgcacagg cggtcgccgg tggcgatctt
      661 ctggccgagt tcgaccagcg gcttcgcgcc cggggccccg gcgcgataga aggtcccgac
      721 catgggcgac ttgaccacgt gaccgtcggg caactcgtct tcggcatcgg tcagggcggg
      781 ggaggcgacg acggccgccg gtgcggctgc gggtgcgggg gggacctggg catagtaggt
      841 tgccggaggt gcgctggtgt gcttggcgat gcgtaccttt tcctcgcctt cggtcacctc
      901 gagctccgag atgccggact cctggacgag atcgatgagt ttcttgagct tgcgcagatc
      961 catggtgttc tccggacggg accgcgatcg cctcgacggg cgccacgatc gggatgattc
     1021 agtgttcggg tttcgcgcac acgccgaggc cgccgcggtc gggcggcgga ctggtgcgga
     1081 aacgtttcgt gctgaggggg ccgcgcgcga actcgctcgg ccgggatggc tcgccaccac
     1141 gggcggaatg cgtgcaagcg acctcgtgaa gcaccgcccc gtcatgccag ggggctggcc
     1201 cgagcgcccg gtgaagatgg gtcgtcaagc attttgagcg cttcatggat gcaagtttgc
     1261 cgtaaatcgc ggcaaattgt ccactttggg ttgtgatctg gacttgcgtg gagggtgcct
     1321 gggacccggc gccgtgcgcg gattctgcgc tcgtgcgccg tgggctgcaa gccgcggagg
     1381 gtgcccggca tggcggggca ggcaaacctc agggagcgtg cgaccccgtg tcgcgtgtgc
     1441 tctccaccgc cgcggcgacc agggcctcga cctgggcgag gcgggcgcga tcctggccgc
     1501 ggcgcgggct gcgctcggcg gccgagggat agatcgacac cttcaccggc acgtcgtccc
     1561 agtcgaagct gaggatggcc tcgggcgtat gcggtgcggg gcggcgcggc tcgcggtgct
     1621 cgtaggcgaa gtcctggttg aggaagaaga tctcgacgtc cttggtgctc tcggggaaga
     1681 gttcgatctc gagttccgag tagcgtccgg cggtgccgtc gagggctccg ccggtgaggt
     1741 aggggcgaaa gtcggcgagc aggcgcatga cctcgatcgc cgcgctgcgc atctcgagca
     1801 ggcgctgcgg ctgctcctcg tcctggtaga tcgcaagcca ggcgcgcagc tcggcctcga
     1861 cctcgttgtt gttgggcagg gcgtcggcgt cggtggcgcc gagctgtttc gcagccttgc
     1921 gcttggcgaa gccgaagtcg ctgatgccgt cctcggccat catgcgtgcg gcgagcgcgg
     1981 cgatctcgcg ccgcaggccg ccgctgcgtg atacgtgggc gtgtgagggc atggtgggcc
     2041 tcgggtagaa tgcgcgccca ttgtaataca cccccggttg cggccggatg aaggcttagc
     2101 ggagcgcgaa cccccatgca catccatatc ctcggcatct gcggcacctt catgggcggc
     2161 gtcgcgctgc tcgcgcgcgc cgccggccac accgtcaccg gctgcgacgc caacgtctac
     2221 ccgccgatga gcacgcagct cgaggagcag ggcatcgggc tgatcgaggg ctacgacacc
     2281 agccagctcg acctcgagcc cgacatgtac gtcgtcggca acgcggtgtc gcgcggcaat
     2341 ccgctcctcg aggcgatcct cgaccgcggc ctgccctacg tgtccgggcc gcagtggctg
     2401 gccgagcacg tgctcgccgg gcgctgggtg ctggcggtcg ccggcaccca cggcaagacc
     2461 accacgacct cgctgctcgc ctggatgctg gaggatgcgg gtttgtcgcc atccttcctg
     2521 gtgggcggcg tgccgcagaa cttcggcgtg tccgcgcgcc tgaccgagtc gcccttcttc
     2581 gtgatcgagg ccgacgagta cgacaccgcg ttctgcgaca agcgctcgaa gttcgtgcat
     2641 taccggccgc gcaccgcgat tctcaacaat ctcgagttcg accacgccga catcttcgcc
     2701 gacctcggcg cgatcgagac ccagttccac catctggtgc gcaccatccc ggcgagcggc
     2761 cgcatcgtcg ccaacgccca cgaggacagc ctgaagcgcg tcatcgagcg tggctgctgg
     2821 tcggagctgg aatggttcgg cggcgacggc cgatggacgg tgggcaccgg ggcggacgag
     2881 gccgaggcgg tgttccgcct cgatggcgtc gagcagggcc gggtgcgcat ggcgctcgcc
     2941 ggcagccaca accgcgacaa cgcgctcgcc gcgatcgccg ccgcgcgcca cgtcggcgtc
     3001 acgcccgcgc aggcgatcgc cagcctcggg cgtttcgccg gcatcaagcg ccggctggaa
     3061 ctgcgcggga cggtaagagg tgtgagcgtc tatgacgact tcgcccacca cccgaccgcg
     3121 atcgcgctca ccgtcggcgg cctgcgccgg cgcgaaccgc agggtcgcat cctggcggtg
     3181 ctcgagccgc gctcgaacac catgaagctg ggcgtcatga aggcgcagtt gccggccagc
     3241 ctcgccgaag ccgatctggt gttctgccat gcgcacgggc tggactggga tgccgccgcg
     3301 gcgctcgccc cgctgggcgc acgcgcgcgt acctacgatc gcctggatgc gctggtcgcc
     3361 gccgtggttg cggcggcaca gcccggcgac cacgtgctgg tgatgagcaa cggcggcttc
     3421 ggcggcgtgc accagaagct gctcgacgcg ctcggagcgt aagtcgcatc ccgggcggcg
     3481 cgttcgaaag gacgccgccg ccagggatgc cggtcggggg cgctcagttg cggcgcgcgc
     3541 cggtcgggtt gtcgaggcgg cgggcttcct cgtcgctgat gtattcgaac acccgcacga
     3601 ccttgcgcac gccttggctg gtgcgcgcca cctcggcggc ggcgtcggcc tcgctgcggg
     3661 ggacgatgcc gagcaggaag acgacgttgg cctcggtcac caccttgacg ttgtgggccg
     3721 agacggcccc ggagtcgatc atgcgcgcct tcacgttgga ggtgatcaag gtgtcgttgc
     3781 cgcgcgcggt gaggctggag ttgggcgcga tggcgacttc gttgatcacg ccgcgcacat
     3841 tctcgacgcc ggccacgatg ccatcgagct ggccgcgcac ggtctcgttg ggcgcttcgc
     3901 cggtgagcag cacgttgcgg ttgtaggagg tgacgttgac atgcacgctg tcgccgaggc
     3961 gctcgcggat gcgcgccgcc accttccatt cgatcccttc gtcctcgaca taggtgccgc
     4021 tggtgcgacg gtcggagacc atcgccgcac ccgcgccgac accggtggcg acgaccggga
     4081 agcagccctg cagcaagggc agtgcgatgg ctgcggaaag gcccaggagg agggtgcgcc
     4141 ggctggactt cgccggtctg tcggcgtgtt ggggtcgggt catcagtctt ccactccgag
     4201 taagaggcag tcgataccgt cgcacaggca gtgcaggatc agcaagtgga tttcccggat
     4261 gcgggcaatc cgctcggcgg gtgcgcagag caggatgtcg gccgggccga gcgtctcggc
     4321 ggtgcgcccg ccgccggcgc cggtgagggc gatcactcgc atgtcgcgct catgggcggc
     4381 gcggatggcg tcgagcacgc gcgccgaatc ggggttggcg gacaaggtca gcaggatgtc
     4441 gccgggctgc ccgaaggcgg cgatctgacg ggcgaacaag gcttgggtat cggggtcgcc
     4501 ggcgatcggg gcgaggctgg agctgtcggc gctcagcgcc atcgcggcga gcgccgggcg
     4561 ttcgagctcg aagccattga ccagctgagc ggcgaaacgg cgcgcgtcgc cggccgaacc
     4621 gctgctgccg cacactagca ccttgccgct gtcgagcagg ctggcggtga tcagctcgac
     4681 cgcgccggcg atcggcgcgg caagcagctc gagcgcctcg agcgaggcgc gcatgttgtc
     4741 ttcgaactgg tgcgagatgc ggtcgatcag gttcatgcgg caagcggcgt ctgcggggcg
     4801 ccagagtcta gcacgcgcta tcagccggca tcgaaggccc ccgggagcca gatgatgcgc
     4861 gccgggtcga gcgcgtcgag cagcaccgcg tcgaagcggc aggctgcgtc gcgaaagcgc
     4921 cgcccggcgc cgcccagcca ccactgcgcg gcgatcagca cgcggcgctg cttggccgcg
     4981 gtgatgctct cggcggcgcc gccgaagcgg ttgttgcggc gcaggcgcac ctcgacgaag
     5041 acgacgtggc tgcggtcgag gcagaccagg tcgacctcgc cgccgcggca gcgcacgttg
     5101 cgcgcgatca cgcgcaggcc gtgtgcggca aggtgttcgg cggcgagcgc ctcggcagcg
     5161 gcgccgcgcg cttgcgccgg cgtcgggcgt gcgccgatac tgtccgcccc cgcgtgcgcg
     5221 ccccgggcgt cctcgcccgc gctgcgcgtc cagcctccgg atctcccgcc catgaccgtc
     5281 tccgctccct tgcccgatcg tccgctctcc agtacgccgt cattgtatgt ggtggcgacg
     5341 ccgctcggga acctgcatga catcacgctg cgcgcgctcg ccgtgctcgc cgcggtggac
     5401 gtggtcgcgg ccgaggatac gcgccacagc cagcgcctgc tcgatgcgca cggtatccgc
     5461 gcgcgcatgc tcgcggcgca ccagcacaac gagcacgagg ccgccggggt gatcgtcgcc
     5521 gagctcgctg ccggccgcca ggtcgcgctg atcaccgacg ccggcacgcc ggcggtgtcg
     5581 gacccgggcg cccgcctggt cgcacgggtg cgcgccgcgg ggcatccggt ggtgccgttg
     5641 cccgggccgt gcgcggcggt ggcggccctg tcggtatcgg gcttcgccga ggggggcttc
     5701 cgcttcgtcg gtttcctgcc ggcgaaggcc ggtgcgcgcg cggcggcgct gcgcgaactc
     5761 gccgacgagc gcgccgcgat ggtgttccac gaggcgccgc accgcatcgt cgagtgcgtc
     5821 gccgacatgg agcaggtcct cggtggcgca cgcgagctgc tgatcgcacg cgagatgacc
     5881 aagctccacg agcagatcgt gcgcatgccg ctggcgcaga ccgccgcctg gttcgccgcc
     5941 gacaccaacc gcctgcgcgg cgagttcgtg cttgtggtgg aaggcgcgcc ggccggcgag
     6001 gtggtggatg cgcgggcgcg ccgggtgctc gatctgctgc tcgccgagct gccggtgaag
     6061 ggggcggcgc ggcttgcggc cgagatcacc ggggcgccgc gcaaggcctt gtacgcgctc
     6121 gcgctcgagc gcaagggcga ggattcgccg caggcgtgac gcatgcacgc ccgcgtcggc
     6181 cgtgcgcacc gccgaagcgc ctataatcgg cgggtcacac gaggaacgcc ccgatgcaat
     6241 cgattaccct gatccgcccc gacgactggc acctgcatgt gcgcgacgac gccgcgctcg
     6301 acgcggtggt gccgcacacc gccgcccgct tcggccgggc gctgatcatg cccaacctga
     6361 agccgccggt caccaccacc gcgcaggcgc tcgcctaccg cgagcgcatc ctcgccgcgg
     6421 cgcgcgggtc gaggttcgag ccgctgatga gcctctacct caccgacaac ctgtcgccgg
     6481 acgagatcga ccgcgcccgc gcgagcggcc acgtggtcgc ctgcaagctc tatccggcgg
     6541 gcgcgaccac gaactccgac gccggcgtca ccgcgatcga caagatctat gcggtgctcg
     6601 aacgcatgga gaagctcggc atggtgctgt gcgtgcatgg cgaggccacc ggggcggagg
     6661 ttgacgtgtt cgaccgcgag cgcgtcttcg tcgagcgcac gctgtcgccg ctggtgcggc
     6721 gtttcccggg gctcaaggtg gtgttcgagc acatcaccac cgcggaggcg gcgcagttcg
     6781 tgcgcgcagc gggctcgcac gtggccgcca cggtcaccgc ccaccacttg ctgctcaacc
     6841 gcaacgcgat cttcgccggc ggcatccgcc cgcaccacta ctgcctgccg gtcctcaagc
     6901 gcgagagcca ccgcgaggcg ctgatcgccg cggtcacctc gggcaacgcg cgctttttcc
     6961 ttggcaccga ctccgcgccg catgcgcgca gcaccaagga aaacgcctgc ggctgcgcag
     7021 gctgctacac cgcgcacgcg ggcatcgagc tctacgccga ggtcttcgac gccgccggcg
     7081 cgctcgatcg cctcgaggcc ttcgccagcc tcaacggccc ggccttctac ggcctggcgc
     7141 cgagcagcga caccatcacg ctgcggcgcg agccctggag cgtgccggcg agctacccct
     7201 acctgggcga agacccgctg gtgcccttgc gcgccggcga gcaggtgggc tggaagctgg
     7261 tggaaggggc ctgagatggg cgtccgcatg cttgagacgg gcgcccggga tgcgggcgcg
     7321 cccatgtcga tccggcccgg cgtccgcggc ggcgccgggc gcgcgctgcg ccgcggtctc
     7381 gctgccgtcg gtctgctcgc cgtgctggcg ggttgcgaga acagcgccac cgcctacatg
     7441 atcgacggca accagcacgc gctgatcctg gtgcgcgagc agaagttctt ctgggatgac
     7501 gagttgcgtc aggcgatggt ggtgtcgcgc ctgcccgcct gtcagaagcg catccgcatc
     7561 catccgggct cggcgaccct cgtcgagatg aagatctacg ccgccggaga cggcctgtgg
     7621 gcgctgcacc agggcgcgcg ttggtatctc gccggtaccg aggagtgccg cgtgcaggac
     7681 tgggacaatc cgggtgaccg cccgcccgga gccctcgtcg gcagcttcgt cctcaaggac
     7741 ggcgcgcccg ccttcgtacc cgcgcccgcc accaaataag ccgggcgacg ccctgccggc
     7801 acgcgtataa tccgggccgg aagtccgcca ggcagtcgct gcgcacctcg tcgtgcgtgg
     7861 aggaaagtcc gggccccgca gagcaggatg ccggctaacg gccgggcgcc gtgaggcgac
     7921 ggaaagtgca acagagagca gaccgccgat ggcccggact tcgctgccgc aaggcagcag
     7981 agtccatttt gaagagcttg ctcttcaatg ggtacaggta agggtgaaac ggtgccgcgg
     8041 cttgcgcgag caagctgagg gtcggtgcgc aagcatcggc aggtaagagc tcaccgcagg
     8101 actggcaaca ggactggcac ggcaaacccc atccggggca aggccaaata ggggagcatc
     8161 ggcgtggctc gcgtcgctcc cgggtaggct gctagagcgc cacggcgacg tggcgcccag
     8221 aggaatgact gcccgatgtc accggtccgc cggtggcgtt cgacagaacc cggcttatcg
     8281 gcggacttcc cacttcttcc cctgtttccg cccgcgcatg ccccctctgc gagcggaagg
     8341 cagcagcgtt ctcccccatc cgctcggaat ccgccagtgc gagcaggccg gattgcaccg
     8401 gcattcccac tttttcccac tgcgcccttt gcgcggcccg atttcgcgtg ccgcgcgaac
     8461 gtccatgcgc atctcccttg atttttcagg atttatccgt gtcttggcgc agagcggtaa
     8521 gtcattgtgc aacaaaggga ttccgatctt gcagccttga cccggtatgg cctttcctct
     8581 aaagtgggtt caagtggaaa aaagtgggga aaagtgtcgt tccgatgctt tcccgaagcc
     8641 gtcagggggt tcgatgttcc agggagcggt ggcgctcaat ctcgatgcga agggacgcct
     8701 tgcgattccc gcgcggcatc gtgatgccct ggccgtcgac aacggccagg tggtgctgac
     8761 cgcccatccg cacggctgct gcctcgtcta tcccgtcccc gcctggaatc cgatccgcga
     8821 ccacgtgctg cgcgcaccga gcctggatcc gcaggcggcg atgctcaagc gcctgctcgt
     8881 gggcttcgcc caggaagaga cgctcgacgc cgccggtcgc gtgctggtgg cgccctccct
     8941 gcgcaagttc gccgcgctcg agaagcaggt gtggctggtc ggccaaggtg cgcacttcga
     9001 gctgtggtcc gacgagcgct gggagaagca gcagcaggcg atgctggaac tggtcaatac
     9061 cggcctgccg cccgggttcg agagcctcgc gctgtgaacc tcgcccatgc ccacgtcagc
     9121 gtcctcctct ccgaagcggt cgacgcgctt gcgatccggg ccgacggtct ctacgtcgac
     9181 ggaaccttcg ggcgcggcgg ccacagtcgc gcggtgctcc aacgcctcgg accgcagggg
     9241 cggttgatcg ccttcgaccg tgatccgctg gcgatcgctg ccgggcaggc ggtgggcgac
     9301 gcccggctga cgctcgtgca ctcggcgttc agcgcgctcg acgacgagct cgcgcgcctg
     9361 ggcgtgcagg cggtggatgg agtgctgctg gacctcgggg tgtcttcgcc ccagctggac
     9421 gacgctgcgc gcgggatgag ctttcgcttc gacgcccccc tcgacatgcg catggatacg
     9481 agccgcgggc agaccgtggc ggagtggctg gcggaggcgt ccgtcggcca gatcacggag
     9541 gtcatcaggg aatatggaga ggaacggttt gctcatgcga ttgcaaaggc gattgcaaat
     9601 gctcgggcag ggggggctgt cgcaaccact ggacaacttg ccgcgctcgt ggaaaaagcg
     9661 gtccgcacgc gcgagccggg gcagcacccg gcgacacgca ccttccaggc tcttcggatt
     9721 ttcatcaatc aggaactcga ggagctgagc cgcgtgctgc ccgtctgcgt gtcccggttg
     9781 cggccgggtg ggcgcctggc ggtgatcagc ttccactcgc tcgaggatcg catcgtgaag
     9841 cgcttcatgc gtgacgagtc gcgtccgccg gtgctgccgc gccggctgcc ggtcagggcg
     9901 gcggatctgc ccgcgccgcg cctgcagctc gtcggcaagg cgatgcgccc gagcgaggcc
     9961 gaggtcgcgg ccaacccgcg cgcgcgcagc gcggtgctgc gcgtggccga gcgcacggag
    10021 gcgccctgat ggtgcgcatc gacgccgtcc tcgtcgccct cgccgtcgcc agcgcgctcg
    10081 gcgtcgtcgc ctcccagcac caggcgcgcc gcctccatgg cgagtacgag cgcgagcagg
    10141 cgcgcgcgca ggcgctcgag gtcgagtggg gccagctcca gctcgagcag agcacctggg
    10201 cgacgcacgg tcgcatcgag aagctcgccc gcgaccgcct tcacatgcag ttgccgcccg
    10261 aagggcaggt gctggtcgtg gagcctcaat catgaaaaag caacgtgccg tcaccttcaa
    10321 tcacaacccg ctgctcaagc gcgagttgcc ggcctggcgg ccgcgcatca tcatgctcgc
    10381 actgatgggt tgttcgctcg cactggtcgg acgtgcgctc tacctgcagg gggtcaataa
    10441 cgacttcctc caggccaagg gcgagtcgcg ctatgcgcgc gtgctcgagg tccccgccac
    10501 ccgcggccgc atcaccgacc gccacggcga catcctcgcg gtgagcacgc cggtgcgcgc
    10561 ggtctgggcc ctgcccgccg atgcccggct ggagccggcg caggtgcgcg agctcgcgcg
    10621 cctgctcgag atgaacgtcg gcgagctcaa cgccaggctc gccgcggggc gcgacttcgt
    10681 ctacctcaag cgccaggtgc cgccggaggt ggcgcaggcc gtcgccgacc tccgcctgcc
    10741 cggcatccac caggaaaagg aataccgtcg ctactacccg ggtggcgagg tgatggcgca
    10801 catcctcggc ttcaccgatc tggaagatcg cggccaggag gggatcgagc tcgccttcga
    10861 caagcgcctg tcgggccgtg ccggcgcgcg ccgggtgatc aaggaccgcc gcggccagat
    10921 cgtcgaggac gtcgaggcga tccgtccgcc acgcgatggc gacgaggtgg cgctgtcgat
    10981 cgacggcaag atccagtatc tcgcctggtc cgccctgcgc gaggcgatgg aagagcacaa
    11041 gcccaaggcc gcggctgcgg tggtgctcga cgtgcgcacc ggcgaggtgc tcgccctggt
    11101 caatgcgccg accttcaacc cgaacaatcg cgccaacctc accggcgcgc aactgcgcaa
    11161 ccgcgtcttc accgacgcct tcgagcccgg ctcggtgatg aagcccttca tcgccgcgct
    11221 cgcgctcgag cgcggcaagg tcaaactcaa taccccgatc gataccggca acggccgcat
    11281 gacgatcggc accgcgacca tctccgacac caagcgccac ggcgtcatca ccatggcgca
    11341 ggtggtgcag aagtcctcca acatcggcac cgtgaagatg gcgcagcagc tcggcgcaga
    11401 ggaaatgtgg cacctcttcg accagctcgg cttcggcacc ccgctcaacc tcggcttccc
    11461 cggcgagact tccgggcggc tgcgtcccgc caagacctgg cggccgatcg agcaggcgac
    11521 gatgtcttac gggcacggca tctcgacgag cctgatccag atggcgcgtg cctacctcgt
    11581 gttcgcgcgc gagggcgagc tggtgccgct gtcgctgacc cgggtggacg gcacgccgag
    11641 ttccgggcgc cgcgtgttct cggccgacac cgcgcgccag atgcgcaccg ttctggcgat
    11701 ggcggcgggt cccggcggca ccgcgatccg cgcccaggtg cccggctaca cggtggcggg
    11761 caagaccggt accgcgcaca agctcgagga tggccgctat gcgcgcaagt acatctcgtc
    11821 cttcgtcggc ttcgccccgg cgagcgatcc gcgcatcgtg gtcgcggtga tggtggacga
    11881 gccttccgca ggccagcact acggcggcac ggtcgccgcg ccggtgttct cgcgcatcac
    11941 cgaaggcacg ctgcgcacgc tcggcgtcgc ccccgacatg cccttgaccc cgctccagct
    12001 cgctcgccgc ggcggcgacg aggcagcgag cgaaggcagc agccgggaga gcatgtgagc
    12061 gccatgcaag ccctcgcggt gctcgatcga ctgcgtgcgc tcggcgtcca cgccgacgac
    12121 atcaccgccg attcgcgcaa ggccggcccg ggttgcgtgt tcgcggcctg gcccggccag
    12181 cgcaccgatg gccgccgctt cctcgccgat gcagtcgtgc atggcgccgc cgcgctgttg
    12241 tgggaggatg gcgacggatt ctgcgcgccc gcgctcgcgg tgcccggttt cggcgtgcgt
    12301 ggcctgcgcg agctcgccgg gcaccttgcc cacgaactcc acggccggcc ttcgcagtcg
    12361 ctttggctag ccggcgtcac cggcaccaac ggcaagacca ccgtctcgca gatgctcgcg
    12421 cgtgcgctcg gcgaactcgg cgagcgctgc ggcatcgtcg gtacgctcgg ctgcgggttc
    12481 cccgacgccc tcgaggaagg gctcaacacc acgcccgacg cgctcgaatt gcagcgctgg
    12541 tttgcgcgct tccgcaacca gggggcggtg gccgcggcga tggaggtgtc ttcgatcggg
    12601 ctcgaccagg ggcgggtcaa tggtgcgcag ttcgacgtcg cgatcttcac caacctgagt
    12661 cgcgaccacc tcgattacca cggcaccatg gaagcctacg ccgcggccaa ggcgcgcctg
    12721 ttcgagcgcc cggagctgtc ggcctgcgtg atcaacatcg acgatccctg gggcctggcg
    12781 ctggcgcgcc ggctcgtcgt cgaggggcgc gcggtgatcg catgcaccct ctacgccgcc
    12841 aacgccgacg cggtgcccgg cgcgcgcgtg ctgcgcgccg atcgcctgca ggcggcgccc
    12901 gcaggactgc gcttcaccct cgtctgggag gggcgcgagg ccgagatcgg cgtgcgcatg
    12961 gtggcgcact tcaacgtctc caacctgatc gcggttgcgg gcgcgctgct ggcgcgcggc
    13021 atcgccttcg acgagctgcc gccggtgctc ggtcggctgg cgccgccgca ggggcgcatg
    13081 cagctcgtcg gcggggtgtg cgagccgctc gtggtgatcg actacgcgca ttcgcccgat
    13141 gccctcgcca aggtgctcga agccttgcgc ggcacggtgc atagccgccg cggccgcctg
    13201 gtgtgcgtgt tcggctgcgg cggcgaccgc gacccgggca agcgcccgat gatgggcgag
    13261 atcgccagcg cgatcgccga tcgcgtagtg atcaccagcg acaacccgcg ttcggaggat
    13321 ccgcagcgca tcgtcgaggc cattgccgcc ggcgccggcg ccgcggccga atgcgtggtc
    13381 gaccgcgcgc aggcgatcac ccgcgcggtg ggcgaggcgc gggccgacga cgtcgtgctg
    13441 atcgcaggca agggccacga gccgtaccag gaaatcctcg gccagcgcct gcctttttcg
    13501 gacctggagc aggcgaagcg cgcgctcgcc gcctggcatg caagggagaa tggccgatga
    13561 tgagcctgca cgacgccacg cgcgcactcg cctgccatgg tgcgcgggcg accggggaag
    13621 ggcgcttcga cagcgtcggt accgataccc gcgcgctcgc gcccgggcag ctcttcgtcg
    13681 ccctgcgcgg cgagcgcttc gacggccacg acttcgtcgc cgatgcggcg aaggcgggcg
    13741 ccgccgcggc catggtcgat gccgcctgga gcgccggcaa ggccgatgcc ggcctgcccc
    13801 tgctggtggt ggacgacacg cggcgtgcgc tcggcacgct cgcggcggct tggcgggcgc
    13861 gtttcgcgct gcccctgatc ggcgtcaccg gcagcaacgg caagaccacg gtgaaggaga
    13921 tgtgtgccgc gatcctgcgc gtgcaggcga gccgcgacgg cttcggcgag gagtcggtgc
    13981 tcgctacgcg cggcaacctg aacaacgaca tcggcatgcc gctcacgctg ctcgaactgc
    14041 gcgacttcca tcgcgccgcg gtgatcgaga tggggatgaa ccatcccggc gagatcggct
    14101 acctgagcgc gctcgcccag cccacggtgg ccctggtgaa caacgcccag cgcgcccacc
    14161 tccagggcat gggctcggtc gacgaggtcg cgcgcgagaa gggcgcgatc tacgaaggcc
    14221 tgggcggggc aggcgtggcg gtgatcaacg ccgacgatcc gcacgccggc tactggcgtt
    14281 cgctcaacgc cgggcggccg attgtgacat tcggtatcga ccatccggcg gacgtgtccg
    14341 cgcggtgtac cctgcgcggc ctcggctcgc ggctcgaact cgacatcccg caagggaaga
    14401 tcgatttcgc cttgcaggtg ccgggtctgc acaacgtgcg caacgcgctc ggcgcggcgg
    14461 cggcctgcct cgcggcaggg gttccgctcg aggcggtggc cgagggactg gcgggtttcg
    14521 ccggcacccg cggtcgcctg caacgccgtg ccgggccgcg gggcgcgctg atcctggacg
    14581 attcgtacaa cgccaatccg gattcggtgc gcgtcggcat cgacgtgctc gccgccatgc
    14641 cgggccatac ctggctggtg ctcggcgaca tgggcgaggt cggcgagacc agcgcccagg
    14701 ttcacgacga ggtcggtggc tatgcgaaga gcaagggcat cgacggcctc ttcgcgctcg
    14761 gcgagatgag cgcggtggcg gcgcacaact tcggcgaggg cgcgcgccat ttcacttcgg
    14821 tggaggcgct ggtcaaggcg ctcgtgccgc gcctggatgc ggactcggtg gtgctggtga
    14881 agggctcgcg cttcatgcgc atggaaaggg tggcggatgc gcttgcggca gtgcacaatc
    14941 cggccccttc aacggaaacc ggctcctgac atgttgttgg aactcgctct ctggctcggc
    15001 caggacattc gtggcttcaa cgtcttcggc tacatcacgc tgcggacggt gctcgccgct
    15061 ctcaccgcat tggcgatctc gctgatcgcc ggccccgccg tcatccgctg gctcgcggcc
    15121 aagaagatcg gccaggccgt gcgcgacgac ggccccaagt cgcacctcac caaggccggc
    15181 acgcccacga tgggcggcgc gctgatcctg atcgcgatcg gcgtcaccat cctgttgtgg
    15241 ggcgacctga agaacgccta cgtgtgggtg accctgctgg tgaccctggg tttcggcgcc
    15301 gtgggctggg tggatgactg gcgcaaggtc gtgcatcgcg atccgaaggg gctggcgagc
    15361 cgctggaaat acctgtggac ctcggcgatc gcgctcgccg cggcgatcta tctcggagtc
    15421 accgcagaca ccccggccga aaccgaactg atcgtgccct tcttcaaggc gattgcgtac
    15481 ccgctgggca tcttcggttt catcgcgctg acctacttcg tcatcaacgg caccagccac
    15541 gcggtgaacc tcaccgacgg cctcgacggc ctggcgatca tgccgacggt gatggtggcc
    15601 gcggcactgg cgatcttcgc ctatgtcgcc ggtcacgtcg gcttctcgaa gtacctcggc
    15661 gtgccctatg tggcgggcgc gggcgaactg gcggtgttct gcggcgcgat ctgcggcgcg
    15721 ggcttgggct tcctgtggtt caacgcctac ccggcggagg tcttcatggg cgacgtcggc
    15781 gcgctcgcgc tcggtgccgc gctcggcacg atcgcggtga tcgtgcgcca ggagatcgtg
    15841 ctcttcatca tgggcggcct gttcgtcgcc gagacgctgt cggtgatggt gcaggtgctg
    15901 tacttcaagg ccaccggcgg caagcgcatc ttccgcatgg cgccgctgca ccaccattac
    15961 gaactgggtg gctggaagga gacccaggtc gtggtccgct tctggatcat caccatcatg
    16021 ctggtgctgt tcgggctgtc gacgctgaaa ctgcgatgag cgcactcacc ggaaaacacg
    16081 tcctcgtcct cgggctcggc gaatcgggcc tggccatggc gcgctggtgc gccctgcgcg
    16141 gggcgcgcgt gcgcgtggcc gacagccgcg ccgtcccgcc cggggtcgac gcgctgaagg
    16201 cggacgcgcc gctggccgag atcttcatgg gcagcttcgg cgacgaggtg ctcgacgggg
    16261 tcgatctggt cgcgctcagc cccgggctcg acccgcgcgt cggggtcgcc gcgcaggcgc
    16321 gtcggcgcgg cctgccgctc accggcgaga tgagcctgct cgcgcaggcg ctcgacgagc
    16381 tcggcgtgcg cgcgcagacg cgcatcctcg ccatcaccgg caccaacggc aagaccacca
    16441 ccaccgcgct caccgcggcg ctggcgcagt cggtcggcat cgacgcggtc gccgccggca
    16501 acatcagccc ggcggcgctc gacgtgctga tggagcgcct ggagatcggc gccgcgctgc
    16561 ccgcgtgctg ggtgctggaa ctctccagct tccagatcga gaccatgcgg ggcctggacg
    16621 ccgaggcggc aaccgtgctc aacgtcaccg acgaccacct cgaccgctac gccgacctcg
    16681 aggaatatgc cgccaccaag gcggcgatct tccagggcga gggcgtgcag gtgctcgctc
    16741 gcgaggacgc gcgcgtcgcg gccatggcgc tgcccggccg gcgcacgatc cgcttcggca
    16801 ccgcggcgcc ggcgagcgac accgactacg gtctggccga cctcgacggc cgcgcctggc
    16861 tggtgcgcgg cggtgagcgt ctgctcgcac tcgccgatct gccgctcgcc ggccgccaca
    16921 acgccgccaa cgcgctcgcc gcgctcgcgc tctgcgaggg cgggctcggt attgcgccgc
    16981 agatcctgat ccccgggctg aaggccttcc gcggactgcc gcaccgggtc gagctggtcg
    17041 ccgagcgcgc cgacggcgtg cgtttctacg acgactccaa gggcaccaac gtcggcgcga
    17101 cggtggctgc gctcgccggc atggactgcc cggtggtgct gatcgccggc ggtgacggca
    17161 agggccagga cttctcgccg ctcgccccgg tactcgccgg caaggcgcgc gcgctgctgc
    17221 tgatcggtcg cgacgccggc cgcatcgagc aggcggtggc gggatgcggc gtggcgatcg
    17281 agcgcgtgcc cgacctcgat gccgcggtgg cgcgcgccaa tgcctgggcg caggcgggtg
    17341 acgcggtcct gctctcgccc gcctgcgcca gcctcgacat gttccgcaac tacgcccacc
    17401 gcgccgaggt gttcgtcgct gcggtgcgtc gcctgcccgg agtgaagccg cgatgaagtt
    17461 cgccagcacc ctcaccggcc tgctccagcg cgaaggtggc gcggccgatc cggcgcgcgc
    17521 ggcgagcggg cgcctcgggc gccagggggc gccggcgcgc gaactcgacc tgctgttgat
    17581 ctgggccgcg gtcggcctgc tgctgctcgg tctggtcatg gtgtattcgg catcgatcgc
    17641 gatcgccgag ggcagccgct tcacggccta ccagtcgcac tatttcctcc tgcgccatgc
    17701 agtcttcctt gctgtcggca tcggcctcgg cctgatggcc ttccagctgc cgatggcgcg
    17761 ctggcagcag ctcgccccgg cgctcttcgt cgccggggtg gtattgctcg tggtggtgct
    17821 catcccgggc atcggtcgcg aggtcaatgg cgcacagcgc tggctctcgc tcgggccggt
    17881 gaacctgcag ccctccgagc tcatgaagat cttcgcggcg ctctatgccg ccgactacac
    17941 ggtgcgcaag ctcgacgtca tgggcagctt cgtgaagggc ttcgtgccga tgatggcggt
    18001 gatcctgctc gtcggcttcc tgctgctgcg cgagcccgac ttcggcgcct tcgtcgtgat
    18061 caccacgatc gccttcggcg tgctcttcct cggcggcgtg aacgtgcgcg tgttcgtgct
    18121 cctcgcggtg gtggcggtga tcggcttcgt gatcctgatc tggacctcgc cctaccgccg
    18181 cgatcgcatc ttcggcttca tggacccctg gcaggacgcc tacggcaagg gctaccagct
    18241 ctcccattcg ctgatcgcct tcgggcgcgg cgagtggttc ggcgtcggcc tcggcggcag
    18301 cgtcgagaag ctcttctacc tgcccgaggc gcacaccgac ttcctgctcg ccgtgatcgc
    18361 cgaggaactc ggcttcgccg gcgtgctgac cgtggtggcg ctgttcgcgc tggtggtgca
    18421 ccgcgccttc atcatcgggc gcgaggcgat caagctcgag cgctacttcg cgggcctggt
    18481 cgcccagggc atcggcttgt ggatcggcat ccagtccttc atcaacatgg gtgtgaacat
    18541 ggggctgctg cccaccaagg gcctgaccct gccgctgatg agcttcggcg gctcgggcat
    18601 cgtcgccaac tgcgtcgcgc tcgccatcct gctccgggtg gactgggaga accgccagct
    18661 cctgcgtggg gggcgtgcat gaagacgctg atggtgatgg ccggcggcac cggcggccac
    18721 atcttcccgg gcatcgcggt ggccgaggaa ctgcgtgccc ggggttggcg catcgtctgg
    18781 atgggcaacc ccgagggcat ggaggcgcgc atcgtgccgc cgcgcggcta cgacaccgcc
    18841 tgggtgcgct tcggtgcgct gcgcggcaag ggcctgctgc gcaagctgct gctgccggtg
    18901 accctgctct ccggcttctg gcaggcgctg cgcgagatcc gccgcgtccg ccccgacgtg
    18961 gtgctgggca tggggggcta catcaccttt ccgggcggca tgatggccgc gctgctcggg
    19021 cgtccgctcg tgctgcacga gcagaactcg gtcgccggcc tggccaaccg tgtgctcgcg
    19081 cgcgtggccg accgcgtgct gagcggcttc cccgaggtgc tggaacaggc cgagtggatg
    19141 ggcaacccgg tgcgcgccga gatcgccgcg gtactgccgc cggccgaacg catggccgga
    19201 cgctcgggcc cgctgcgcgt gctggtggtg ggtggcagcc tgggcgccgc ggtgctcaac
    19261 gagaccgtgc cgcaggcgct gctgcgcctt cccgccgagc agcgcccggt ggtggtgcat
    19321 caggctggcg aacgccagat cgaggccttg cgcgcggcgt acgcgcgcgc ccaggtcgac
    19381 ggggagctgc gccccttcat cgacgacatg gcggcggcct acgccgacgc cgacctggtg
    19441 atctgccgcg ccggcgcgct caccgtggcc gagctcgccg cggccggggt ggccagcctc
    19501 ttggtgccct tcccctacgc ggtggacgac caccagaccg gcaacgcgcg cttcctcgcc
    19561 gaccgcggcg gcgcctatct gctgccgcaa tccgaactaa cgcccgaccg cctggcgggc
    19621 atcctgtcca gtcttgaccg cggccgcctg ctgcacatgg ccgagaacgc gcgcgcgctc
    19681 gcgaagccgc tcgcggccgt cgcggtggcg cacgtgtgcg ccgagctggg cggtggagaa
    19741 caagcatgaa gcacaaggtc aggaacatcc actttgtcgg catcggcggc gcgggcatga
    19801 gcggcatcgc cgaggtcctc gccaaccagg gttatcgcgt cagcggttcg gaccttggcg
    19861 acaacgccgc cacccgccgc ctgcacaaga tgggcgcgca cgtggtgcgc gggcatgacg
    19921 cggccaacgt cgccgacgcc gacgtggtgg tggtgtcgac cgcggtgaag gccgacaacc
    19981 cggaggtggt cgcggcgcgc gagcgccaca tccctgtggt gccgcgcgcg cagatgctcg
    20041 ccgagctgat gcgcttcaag cacggtatcg cgatcgccgg cacccacggc aagaccacca
    20101 ccacctcgct ggtggcgagc atcctcgccg agggcggcat cgaccccacc ttcgtgatcg
    20161 gcgggcgcct caacgccgcc ggcgccaacg cgcgcctcgg cacgggcgac tacctggtgg
    20221 ccgaggcgga cgagtccgac gcctccttcc tgctgctcaa cccggtgatc tcggtggtca
    20281 cgaacatcga cgccgaccac atggacacct acggccacga cttcggccgg gtcaagcagg
    20341 ccttcgtcga cttcctccac cgcctgccct tctacggcgt ggcggtgctg tgcgaggacg
    20401 accccaacgt gcgcgagatc atcccgcagg tggccaagca gatcgtgcgc tacggcttct
    20461 cggagaccgc caacatccgc gccgagaacg tgcgcgccga gggcgggcgc atgcggttcg
    20521 actgcgtgcg ggtcaacggc acgacctcgc gcctgtcgat cgagctcaac ctgcccggcc
    20581 tgcacaacgt gctcaacgcg ctcgccgcga tcgcggtggc caccgaggtg caggtgccgg
    20641 acgcggcgat cgtcaaggcg ctcgccgagt tcaacggcgt cggccggcgc ttccagcgct
    20701 acggcgaggt cgcgctgcac gaccaggacg gcgatgaggc cggccgcttc acgctggtcg
    20761 acgactacgg ccaccacccg gtggagatgg cggcgaccat cgccgcggca cgcggcgcct
    20821 tccccggccg ccgcctggtg ctggccttcc agccccatcg ctacacgcgc acacgcgatt
    20881 gcttcgagga cttcgtcaag gtgctgtcga ccgtggatgc gctgctgctg gccgaggtct
    20941 atgccgccgg cgagaccccg atcgtcgccg ccgacggccg ctcgctcgcg cgcgcgatcc
    21001 gcgtcgccgg cacggtcgag cccgtcttcg tcgaggagat cgccgacatg ccgcgcatga
    21061 tcctgtccgc cgcgcgcgac ggcgacgtcg tcatcaccat gggagcgggc agcatcggcg
    21121 cggtgccggg caagctcgcc agcatcgagg aagcagcgtg aaggcccggt tcggaaaggt
    21181 tgcggtgctg ttcggcggtt cgtctgccga gcgcgaggtt tcgctgatgt cgggcgctgc
    21241 ggtgctcgcc gcgctgcagg ccgcgggggt ggatgcgcac gccttcgatc ccgcggagcg
    21301 cgacctgcac gtcctcaagg aagagggctt cgagcgcgcc ttcatcgccc tccatgggcg
    21361 cggtggcgag gacggcacgg tgcagggcgc gctcgagctg atgggcatcc cctataccgg
    21421 cagcggcgtg atggcctccg cgctgtcgat ggacaagtgg cgcaccaaga tggtgtggct
    21481 gtccgccggc ctgccgacgc cgcgctacgc gatcctggag cacgactccg actgggacgc
    21541 ggtggtggcc gaactcggcc tgccgatctt cgtcaagccg gtgcacgagg gctcgagcat
    21601 gggcgcgacc aaggtggtcg aggcgggcca gctgcgcgcc gcgtgggagc tcgcggcgcg
    21661 ctacgaccgc ctggtgatcg ccgaggagtt catctgcggc gccgagctca ccgccccctt
    21721 cctcgaggac cgcgcgctgc cgctggtgcg catcgtcgcc cccggcggca actacgacta
    21781 ccagcacaag tacttcaccg acgacacgcg ctacgactgc cccagcggcc tgcctgccgc
    21841 gcaggaggcg gagctgcagg cgatggtgat gaagtgcgcg cgcgtgctcg gctgccgcgg
    21901 ctggggccgc gccgacctca tcctcaccga ggacggccgc ccctacctgc tcgagatgaa
    21961 cacttcgccc ggcatgaccg gccactcgct ggtgccgatg tcggcgcgcg tggcggggct
    22021 ggacttcacc gcgctgtgcc tcgagatcct gcagggagcc cgccttggct gaagccggca
    22081 tccgtcccgc gtcgatccgc tccgcccgcc gtccggcggc gggcggagcg gcgcgcgcgg
    22141 gtgcggagcg cggcctgtgg catcggcccg cgttgctcaa cctgatctcc gacctgctca
    22201 ccctgggtgc ggcggtggcg ctcggctggg cgctggtgat ctggttcgtg tcgcggccgc
    22261 tgttcccgct gcgcgagctg gtggtgctca ccgaccccgg ccaggtcacc gtcgaacagc
    22321 tcgactacgc cgcccgcctc gcggtgcagg gcaacttctt caccgtcgac ctcgacggcg
    22381 tgaaggaaac tttcgagaag ctgccctggg tgcgcaaggc cgaggtgcgc cggcgctggc
    22441 ccgacgcgct cgagctgcgc ctggaagaac acgaggcggt ggcctactgg acggtgtccg
    22501 agagcggcga ggcgcgcctg gtcaaccgcc agggcgaggt tttcgtcgcc gcttccaacg
    22561 ccgacatgcc ccagttcgac ggcccgcagg ggtcggcggg ctggctgctc gcacgccatg
    22621 ccgagttctc cagcatgctg cagccgctcg gcgtgcgcct ggtcgggctg gccttgtcgg
    22681 cgcgcgaggc ctggcagctg cagctcgata acggcatgac catcgtgctc gggcgcgagc
    22741 aggacaagtc tccgctgatg gaacggctgc gccgcttcat cgcggtgtgg ccgcgcgttc
    22801 acgaacagat cgacatcgac atcaagaccg cggacctgcg ctatgcgggc gggttcgcac
    22861 tgaccccggc cgacgcctcg gtgctccacc agcccgtcgc gcccgccgcc cggaaaggca
    22921 ggaaatgagc aagcaataca acgaactgat cgtcggcctg gacatcggca ccgccaaggt
    22981 cacctgcatg gtcgccgagg tgaagcccga cgggcgactc aacgtcgtcg gcctcggcac
    23041 ccagtccacc aacggcctca agcgcggcgt ggtggtgaac atcgaggcca cggtggacgc
    23101 gatctcgcgc gtggtgcagg aggtcgagct gatggccgac tgcaagatcc acgaggtcta
    23161 caccgggctg gccggcagcc acatcaagag cttcaactcc agcggcacgg tcgcgatcaa
    23221 ggagaaggag gtctcgccgc tcgacgtcga gcgcgtgatc gaggtcgcgc gcgcgatgcc
    23281 gatcccggcc gagcagcaga tcctgcatat cctgacccag gagttcatca tcgacggcca
    23341 gggcggcgtg cgcgagccga tcggcatgag cggggtgcgg ctcgaggtca aggtgcacat
    23401 cgtcaccggc gcggtgtcgg cggcgcagaa cgtgatcaag tgcgtgcgcc gctgcgggct
    23461 ggaggtcatg gatctgagcc tgcagccgct cgcctccagc cacgccgtgc tgaccgagga
    23521 cgagaaggaa ctcggcgtgt gcatggtcga tatcggcggc ggaacgaccg acatcgccgt
    23581 gttcacccag ggcgcgatcc gccataccgc ggtgatcccg gtcgccggcg accagatcac
    23641 caacgacatc gccatggcgc tgcgcacgcc caccgccgag gcggaggaga tcaagatccg
    23701 ccatggcgtg gcgatgcacc acatggccga ccccgaggag atgatcgagg tcccgggcgt
    23761 gggcgatcgc ccgccgcgca agctctcgcg ccaggcactc gccgacgtga tcgagccgcg
    23821 cgtctccgag ctcttcgagc tggtgcaggc cgaactgcgc cgcagcggct acgaggagct
    23881 gctgtcctcg ggcatcgtgc tgaccggggg ctcggcggtg atgcgcggca tggccgagct
    23941 cgccgaggac gtcttccaca tgcccgcgcg cgtgggcgcg ccgcagtacg acggcggcct
    24001 tgccgacgtg gtctgccagc cgcgctacgc caccgcgatg ggcctggtca tggagggcgc
    24061 cgtgcagcgt cgcagggggc tgcaggcgcg cgacacgcgc agcgtgaaac agtttttcgg
    24121 gcgcatgaag gcctggttcg aacgcaattt ctgaacgggt tttcgggcgg ttttcatttt
    24181 ggatgtgggc cgatcaccat cgcttattta gtagtaaact cccggccgaa ttactagatg
    24241 caggccggct ggcgatcggt gccgcgcggg gcagtcaatt caggaggcga ggctaatgtt
    24301 tgaaatcgtt gagaaggaag ccaccggtac ggtgatcaag gtggtcggcg tcggcggtgc
    24361 gggcggcaac gcggtcgacc acatgatccg cgagggcgtg aagggagtgc atttcatctc
    24421 cgccaacacc gacgcgcagg cgctcaagcg ctgcctcgcc ccggtcaagg tccagctcgg
    24481 catcaccggc ctgggtgccg gctccaagcc ggaggcgggt cgcgccgccg cgcaggagtc
    24541 gcgcgatgcc atctccgccg cgctcgaggg tgcgcacatg gtgttcatca ccggcggcat
    24601 gggtggcggt accggcaccg gtgcggcacc ggtggtggcc gagatcgcca aggagatggg
    24661 tctcctgacc gtcgccgtgg tgaccaagcc gttcgatttc gagaaccgca tccgcgtcgc
    24721 cgagagcggc atcgaggaac tcacccgcca cgtcgactcg ctgatcgtcg tgctcaacga
    24781 caagctgctc gaggtcttcg gcgacgacgc cggctttgag gagtgcttcc gctcggccga
    24841 caacgtgctg cgctcggccg tgggcggcat cgccgagatc atcaacgtgc ccggcctggt
    24901 caacgtcgac ttccaggacg tccgcaccgc gatggccgag atgggccgcg cgatgatggg
    24961 ctcggccgag gccgccggca tggaccgcgc acgcatcgcc gccgagcagg ccgcggtgtc
    25021 gccgctgctc gagggcaccg agctctccgg cgcgcgctgc gtgctgatca acatcaccgc
    25081 gagcaagtcg ctgaagatgt ccgaagtgcg cgacgcggtg aagaccgtgc aggccttcgc
    25141 cgcgcccgaa gccttcgtca agtacggcac cgtgttcgac gacaccatgg aagaccgcat
    25201 ccgcatcacc gtggtggcca ccggactcgg cgcgccgcgc gccgcgcgcc agccggtgat
    25261 gcaggtggtg cagggcaccg gcacctacgg cccggccatg ggtggcgcgg tcgacgtgac
    25321 caatctcgac gtgccggcgg tgatgcgcag cggtcgccgc accaccgtcg aggcgatgag
    25381 caccagcggt gtgggcacct acgacatccc ggccttcctg cgccgccagg cggactgacc
    25441 tccgcccggg tggccgccgc cgcggcggcc acgaaccgga acccccgcac gggccgtcgc
    25501 ctccggcccg tgcgggggtt ccgccgttta ccggctggcg tcgggcgcgc aacatccgct
    25561 tgcgcaatag cccggatgca tcggcgcgat cgccgggggg ctgcgggggt gcatgttaaa
    25621 atcgcgcatc gaagaggaaa aagaccatga tccggcagcg caccctgaaa tccgtcgtca
    25681 cggcgaccgg cgtcggcttg cacggcggtc gcaaggtgac cctgaccctg cggccggcgg
    25741 cgcccgacac cggcatcgtc ttccaccgcg tcgacctcga cccgcccctg acccttcctg
    25801 ccgatcccta tgcagtgtgc gacacgcgca tgtgctccgg gctggagcag ggcgggcaca
    25861 aggtcggaac ggtcgagcac ctgatgtcgg cgctggccgg gctgggcatc gacaacctgc
    25921 atgtggacgt cgatgcgccc gagatcccga tcctcgatgg cagctccgcc cccttcgtgt
    25981 tcctgctcca gtcggccggc atcgaggagc agagggcggc gaagaagttc ctgcgcgtga
    26041 agaaggcggt cgagtacgtg gagggcgaca agtgggtccg gctcgaaccc tacgatggct
    26101 tccgcctggc gttctcgatc gtgttcaacc atcccgcgat cgacagcacc tcgacccggg
    26161 tcgaggtcga tttcgcccgt gactcctaca tacgcgacgt cgcgcgcgcg cgcaccttcg
    26221 gcttcatgca ggacgtcgag ttcatgcagg ccaacgggct ggcgctcggc ggcagcctgg
    26281 agaatgcgat cgtgatggac gagtaccgcg tgctcaatgc cgagggcctg cgctacgccg
    26341 acgagttcgt caaacacaag gtgctggacg cgatcggcga cctctacctg tgcggccacc
    26401 ccttgctcgc gtcgtattcg gcgcacaagg ccggccatgc gctcaacaac cagatcctgc
    26461 gcgtgctgct cgaggacaag gaggcctggg agctggtgag cttcgaccag gaggccgcga
    26521 ccccgcccgc ggtctcgcac cagttcgcgc cgctcgtagc cctcgcatga acgggcgctt
    26581 cgtgtccggt cgtatcccgg tgatcgcacg cgccgcgcct ggcctcgatc ccggctgcat
    26641 gcgcgccgcc gctgcgggcg aggcggtcga cgcgcgccgt cgaggggcgt acgcatgctg
    26701 atcatgcgcc tgctgctgtt gctcgcggtg ctgtcgatcg gtggctcggc gatcctgtgg
    26761 ctgctcaccg gcaacccgcg ctacaaggcg tgggcatgga aggccttgcg cgccaccttg
    26821 gtgatgctgt tcgtgctgtt cgcgctgttc gccatcgagc gcgtgctcgc cccggtcgcc
    26881 tgacttccgc ggggggcggc gcggaccgga gcgccgatat cgcgactcgc gtcgctccta
    26941 cggggcggtg cggaccgcag cgccgcgatc gcgacttgcg gcgctcctac gcggggtctg
    27001 cgagggcgtc gcggttctcg taggagcgac gcaagtcgcg atccctccgt tcggcgaccg
    27061 gcgtgcgcct cttcaggcgc gccggatcag gcgttcgatg gaggcgcgca gcggcgagtc
    27121 gacgggcagg ctgtcggcga agtcgtgcag ctcggccttc gcggcctccg agatctgtcg
    27181 ctgcggagcg tggcgttcgg gagccgtctg ctcgggcgtg ccgaccttga ccttgatcga
    27241 ctgcaccgga tgtccgccat gctgcaggcg ctccagcagg ctgggcagga tctggcgcac
    27301 tcgcaccgcc gccgcgccgc cgcgtgcggc cacgaccagg ctgccttcct tgaggttcgc
    27361 gacccggcac tggccctgca gctgtggcgg aagcatgccg tcgagcaccg cctgcagccg
    27421 gttcaggcgc gcggcatggt cttgcaggcg ggcgagggcg tcgccgccga gaaagcgtga
    27481 gagcagccgg gtcatggatg cgtgcgagaa ccgtcacggg gagcctgggt gcgccatgat
    27541 aaactcccgc ctttgccgaa tcgacgtcga cccaaaaaca tgatctccgg cctgctcaag
    27601 aaaatcttcg gtagtcgcaa cgaccgcctc gtccgccagt acatgcacac cgtgcgcaag
    27661 atcaacgcct tcgaggcgga gatctccgca ctgtccgacg aggcgctgcg cggcaagacg
    27721 gccgagttcc ggcagcgcgt ggccgacgga gagtcgctcg acgcactgct gcccgaagcc
    27781 ttcgccgtgg tgcgcgaggc cggcaagcgg gtgcatggca tgcgccactt cgacgtccag
    27841 ctcgtcggcg gcatggtgct gcaccacggc aagatcgccg agatgcgcac cggcgagggc
    27901 aagaccctgg tcgccacgct gcccgcctac ctcaacgcgc tgtcgggcaa gggcgtccat
    27961 gtgatcacgg tgaacgacta cctcgccagc cgcgacgccg agtggatggg gcgcatctac
    28021 ggcttcctcg ggctcaccac cggctgcaac ctgtcgcgca tgtcgcacga ccagaagcag
    28081 gccgcctacg ccgccgacat cacctacggc accaacaacg agttcggctt cgactacctg
    28141 cgcgacaaca tggtgtatgc ggtcggcgag cgcgtgcagc gcgcgctcgc cttcgcgatc
    28201 gtggacgagg tcgactccat cctgatcgac gaggcgcgca cgccgctgat catctccggc
    28261 caggccgagg accacaccga tctctacctg aagatgaacc aggtcgcgcc gatgctcaag
    28321 cgccaggaag gcggcctcga cgacaaggac gaggtcatcg aggcgggcga ctacacggtc
    28381 gacctcaagg cgcaccaggt gctgctcacc gaggcgggcc acgagaccgc cgagcagatc
    28441 ctcgtgcgca tgggcctgct tgccgagggc ggcggcctct acgaccccgg caacatcctg
    28501 ctggtgcatc acctctacgc ggcgctgcgt gcccacgccc tctaccaccg cgatcagcag
    28561 tatgtggtgc agaacggcga ggtcgtcatc gtcgacgaat tcaccggccg cctgatgccc
    28621 ggccggcgct ggtccgacgg cctgcaccag gcggtcgagg ccaaggaagg cgtgcgcatc
    28681 caggccgaga accagaccct ggcctcgatc accttccaga actacttccg catgtacggc
    28741 aagcttgcgg gcatgaccgg tacggccgac accgaggcct tcgagttcca gcagatctat
    28801 gggctcgaga ccgtggtgat cccgaccaat cgcccgaccc ggcgcaggga cgagaacgac
    28861 aaggtctacc gcaccgcgag cgagaagtgg aacgcggtca tcgaggacat ccgtgcctgc
    28921 gtcgagcgcg gccagccggt gctggtcggc acgacctcga tcgagaccaa cgaattcctt
    28981 tccggcgtgc tcaagaaggc gaagatcgag caccaggtgc tcaacgccaa gcagcacgac
    29041 agcgaggcgc agatcgtcgc gcaggccggc cggcccggca tggtcacgat cgcgaccaac
    29101 atggccgggc gcggcaccga catcgtcctc ggcggcaaca tcgagaagcc ggtcgccgcg
    29161 gtgcgcgacg acgccacgct cgaccccgtg cagcgcgaga ccaggatcgc cgccctgcgc
    29221 gaggcctggc agaaggtgca tgacgaggtg atcgccgccg gcggcctgca catcatcggc
    29281 accgagcgcc acgagtcgcg ccgcatcgac aaccagctgc gcggccgctc cgggcgccag
    29341 ggcgacccgg gctcgagccg cttcttcctc tcgctcgaag atccgctgat gaagatcttc
    29401 gccggcgagc gcctgaacgc gatcatggtc cgcctgaaga tgcccgaggg cgaggcgatc
    29461 gagcacgcga tggtgacgcg ctcgctggag tcggcgcagc gcaaggtcga gcagcgcaac
    29521 ttcgacatcc gcaagcagct gctcgagtac gacgacgtcg ccaacgacca gcgcaaggtg
    29581 atctaccagc agcgcaacga gctgctcgag agcgaggaca tctccgacac cgtgcgcgcg
    29641 atgcgccagg gcgtgctgca cgacatcttc cgccgctacg tgccggtcga cagtgtcgag
    29701 gagcaatggg agctgcccgc gctcgagcag gcgctgctcg ccgagtgcca gctcaagctg
    29761 gcggtggccg cgtgggtgac ggccgagccc agcctggacg acgaggccat cctcgagcgc
    29821 gtggtgcagg ccggcgagga ggcctacgcc gccaagaccg cgatggtcga cccggcggcc
    29881 tggcaccagt tcgagcgcaa cgtgatgctg cagagcctcg acacccactg gcgcgagcac
    29941 ctcgcggcgc tcgaccacct gcgccagggc atccacctgc gcggctatgc gcagaagaac
    30001 cccaagcagg aatacaagcg cgaggccttc gagctcttcg agagcctgct cgacgccgtg
    30061 cgcaccgacg tcaccaagat cctgatgacg gtacagatcc gcaccgaggc gcagctcgag
    30121 gaagccgaga cgccgcagca ggtcgagaac gtgcagtacc agcacgccga ctacgacgag
    30181 gcgctcggcg gtggcgccga gcaggcgggc gcgcagccgg ccactgccgg gcccaaggtc
    30241 ggccgcaacg acccctgccc ctgcggctcg ggcaagaaat acaagcactg ccacggcaaa
    30301 ctgaactgat ccttccgtcc cggagaaccg catggccgtc aatctcgcca ccccgaaccc
    30361 tgccgaactg ctgcccgtga agggcgtgcg cctcggcgtg accgaagccg gcatccgcaa
    30421 ggccaaccgg cgcgacctca ccgtgatcga gctgtccgag ggcagccgcg tcgccggcgt
    30481 gttcacgcag aaccgcttct gcgcggcgcc ggtgcaggtg tgccgccagc atctgacggg
    30541 cggggcgatc cgcgcgctgc tcatcaacac cggtgtcgcc aacgccggca ccggcgagcc
    30601 gggcctggcc aacgccaatg cctgctgcgc cgcgctggcg cgcgaactgg gcatcgaggc
    30661 gggtcaggtg ctgccgttct cgaccggcgt gatcctggag ccgctgccgg tcgaccgcct
    30721 ggtcgccggg ctgcccggag cggtcgcaga cctgcgtgcc gacggctggt tcgatgccgc
    30781 gcacgcgatc atgaccaccg acaccctcgc caaggcggtg tcgcgccagg tccgcatcgg
    30841 cgagcgcacg gtcaccctca ccggcatcag caagggcgca ggcatgatca ggcccaacat
    30901 ggccaccatg ctcggcttcc tcgcctgcga cgcggcgatc gcgcaggacc tgctcgatga
    30961 cttggtgcgc gaggcggccg acctgtcctt caacagcatc accgtcgatg gcgacacctc
    31021 gaccaacgat tccttcatcc tcatcgccac cggcgccgcc ggcaatgccg agatcgccga
    31081 cgccgacagc gaggactacc gcgccctgcg cgcggcggtg atcgaggtcg cggtcgggct
    31141 cgcgcaggcg atcgtgcgcg atggcgaggg cgcgaccaag ttcatgacga tcgcggtcga
    31201 gggcggccgc gaccgcgagg aatgccgcaa ggtgggctat gcggtggcgc actcgcccct
    31261 ggtcaagacc gcctttttcg cctccgaccc caacctcggc cgcatcctcg cggcgatcgg
    31321 ctatgccggc atcgacgacc tcgacgtcgc caagctgcgc gtgtggctgg gcaaccccga
    31381 ggagtcggtg ctggtcgccg agcacggcgg tcgtgcggcg agctacctcg aggaggcggg
    31441 ctcgcgcatc atgaagcacg ccgagctcac ggtgcgcatc gcgctcgccc gcggcgacgc
    31501 cgccgccacg gtgtggacct gcgacttctc ctacgactac gtgaagatca acgccgacta
    31561 ccgcagctga gcgccttccg gcgtgcgcca cggccccggc agccactcgg cgccggggcc
    31621 gtgtcatgtc atgcagggcg ggcggcgacc gtgtgctggc atgcgtgacg tggttcaccc
    31681 aaggcggcgc acgtatcttg tcggcgcccc cggaatgggc agaatgcagg ctggatggtg
    31741 cgctcagttc gccatggagc cgtaggaatc gctttgtcat gcaggatccg tataccagtc
    31801 gcgacccgcg cacgcggggt ctggcttcgc gcgcggggcc cctcgtcctg cttggcctgc
    31861 tcgcgctcga cgcgcgtgcg gaatggggcg acccggaccc gttcttcagc gagattccga
    31921 tggtgctgac cgcatcgcgc ctggcgcagt cgccgctcga cgcgcctgcg ccggtgaccg
    31981 tgatcgatcg cgagatgatc gtcgcctcgg gtttcaacga gatccacgac cttctgcgcc
    32041 tggcgcccgg cttcctcgtg gccgaccatc ccgatggtcc ccccacggtg gcgacccacg
    32101 gcctgggcga cgcccatgcg cgccgcatca aggtgatggt ggacgggcgc accgtcaaca
    32161 gcccgctgtg gggcaacgtg atctgggaca acctgccctt gcgggtggac gatgtcgagc
    32221 gcatcgaggt ggtgcgcggc cccaacggtg cggcctatgg cgtcaatgcc ttccaggggg
    32281 tggtcaacat catcacgagg gctccctcca ccgagagcgg gcgggcgttc gtcgtgcgca
    32341 ctggaaagaa cggtttccgt gattacggcg tgcgcctgaa cggggctggc ggaagcgcct
    32401 tcgactggcg cctctccgcc tcgcggcgcg agctgacgac gttcgaaccg cactttcggg
    32461 aacggctcgg ggactggcac tcttccgagt cgctcacccg caacaccgcc cacttcagcg
    32521 cagtgctgca gttgtccgca gccgaagagc tcggcctcca ggttggcgtc agcgagggcg
    32581 acagcaagcg cggcgcccag gcctacctcg actatccgcc ccacaccgag gaggaccgcg
    32641 cgcttcacct gcaattcggc tggcggcgcc acatcgatgc ggaatccgtg ctgagcgtgc
    32701 agtatttcca ccagggcgag cgtgccggcg ggagctggcc ggtgatccag gcgcaaggca
    32761 cgacgaccgc gagcttcccg accagccggg ccagcgaggc gaggcggcac gatctcgagc
    32821 tgcaatacga cacccggctc ggccggcgtc tcaccggcat gctcggtatc gggctgcgca
    32881 gcgagcatgc gcgctcgctg cacctgttcg gcaccacacg gtggctggat tcgacccatg
    32941 ggcaggtctt cggcagcctc acgtggaacc cgctcgatcc gctcaagatc gatctcggcg
    33001 gcacgctcga gcaccacgac tacagcggca ggctgttctc gccccgcctt gcgttcaact
    33061 acgccctcga cgaggactcg gccctgcgcc tgtccaccgg gacggcgtac cgcgccccca
    33121 gcctgatgga gtcgcgagcc ttccagaccg accgcgaggg cgacacgatc cggcgtatcg
    33181 gcttccgtgt cttcgacccg ctgcagagcg agcgcatgcg cttcgtcgat ctgggctatg
    33241 tcgcgcagct gcgttcgttc ggactcggct tcgatgcgcg tgtctttcgc gagtactacc
    33301 ggcgtttcat cgacgacaag agctgcgtgt tcggagaatg cccgcgcccc gagccggccg
    33361 actatgttcc gatcgacccc ggcaagaaga gcttctattt cctcaacgtc gacgacttcc
    33421 gcatgaccgg tgcggaattc acccttgact ggcgcaagcc gggctggggc agggcggtac
    33481 tgtcccaggc cttcatccgc atcgaccatg gcgagcccta cagcgaccgc gacatcccga
    33541 acagcgcacc gcatgcgacc acctcgctgc tgctgatcaa ggacttcccc ggacgctggc
    33601 aggcgagcct gggctactac cacaacggcc tgtactactg gatgaacggc ggcgacatcg
    33661 tgccgcgcac cgatcgcttc gacctgcgcg tcgcgcgccg cttcggtccg cccggttcgg
    33721 acaacgaggt ctcgctcacc gcgctgagcc tgaaggggcg ctacgcggac ttccacgagg
    33781 acaagttccg ccacgagccg gacctctatc tcggcgtgcg tatcggatgg tgacggcgcg
    33841 catgcgagcc tgcctgtcgt gtctcctccc cggcggcctg cgcgtcgcag cggcgctcgc
    33901 catgctcgcg ctgctgcctg tgctcggcct gtccgttgcg cacgcgatgg aggtggcggt
    33961 gctggtttcc gagccggggg ggcgcggcca gcgcttcgtc gaggcgctcg atgccgcgct
    34021 cgcgcccggg gtgcatcggc tggcaggcgc ggggctcgcc ggcggggagg ccgatacggc
    34081 gctgctcggc cgcgccgatc tcgtgctcgc ggtcggccac gcggcggcgg agcgcgccct
    34141 gcgggattcc tcgcggccgg tgctggccgt gctcgtcggc ctgcgcgacc tggatcgcct
    34201 caagcgggcg catcccgcgc gggatctcgg cgcggtggtg ctcgaccagc cgctcgaacg
    34261 tcatctccgt ctcgtgcgcg cggtcctgcc cgacgcgaag cgcgtcggcg tgctgctcgg
    34321 accggacagc gtcgtgcaac tgcccggcct ccagcatgca gcggacgcgc tgcgcttgca
    34381 ggccgacgtc catgaggtgc gcgaggtggc ggacctcgtg ccggcgctcg atcacctgct
    34441 ctttgccggc gacgccctgc tgcccctgcc cgacggcctt gtctcggggc cggccgcggc
    34501 gcgcacgatc ctgctcacca gttaccgcca ccgccggccg gtgttcgccc actcggccgc
    34561 ctatgtcacc gcgggtgcgc tcgccgcggt gttcagcacg ccagagcacg tcgcggtgga
    34621 tgtcgcggac ctgctggcgg cagccaagga gcctgcggcc ctggtgcgca agatccactc
    34681 gccgcgccgc ttcgatgtcg cggtcaatcg cgcggtggcg cgggcgctgg ggctcgtggt
    34741 cgcggacgag caggtgttgc gcgagcggat tgccgacgga ggggcgcaat gatcgccgcg
    34801 ctttcccgtt actcgctgcg gcgacagatg ctgttggtgc tcggcctgcc gagcgtcgtg
    34861 ttcgtgctct gcatcgccgc gctcttcgcc taccagagca gtcgcgcgct cgaccaggcc
    34921 ctgctcgaac gcggccgtgc catggtgcgc ttcctcgcac cggcggctga atacgtgctc
    34981 gtgtcgggca accgcgaagc gctcggcgcg ctgatggcgc gggtgcaggt acagcccgac
    35041 gtggtcggcg tcgggttcta cgatccgggc ggcgacgcgc tggcgacgct gggagagggg
    35101 ttgtcgccca ggctggccgc gccgtcgcgc gccgaggggg acgagatccg tgtcgacctc
    35161 gcggacggtg cgatcggctt cgaggcgccg gtggtggcgg tgcccgcgac ggtggacgac
    35221 tatgcggagg attcgtcgtc gaagacggcg ccggtcgtcg gccaggtccg cgtggtgctc
    35281 ggcaccgcgc gtctggatgc cgagaagcgc ggggtcttcc tgaccgtgag cctgttggtg
    35341 ctgggcgtgc tcgcgacggc gatggcaatc gcggtgcgtc tggccggatc ggtcggcgag
    35401 ccggtcgctg ccctggtgga tgcggtcggg cgcatggccg acggcgacct cgcggcgcgc
    35461 gtaccggcgc gtgcgcgcaa cggcgagttg aaggcgctcg aagcgggatt caacgccatg
    35521 gccgaggcga tcgccgagaa ccagcgcacg ctgcaggccc gcatcgacga ggccaccgtg
    35581 caactggcct ggcaggcccg ccacgacccg ctgacggggc tcgccaaccg gcgcgccttc
    35641 gaggagcgca tcgagcaggc ggtgcgtgcg caccggcgct cgggcgacca gatcaccctc
    35701 ttctatatcg acctcgatca cttcaaggtg gtcaatgaca gctgcggcca tgctgcggga
    35761 gaccggttgc tgagcgagct cggtgccctg ctcaagggtc gcctgcgctc cgaggacgaa
    35821 gtgttccgca tcggtggcga cgagttcgcc gcgatcttgc gcggttgtgg ccgcgaggac
    35881 gcgcgccgca tcgccgaggg gctgtgcgcg gcgatcgccg cgcatgcgtt cgagtccgac
    35941 ggcagggcct tccgcgtcgg cgccagcatc gggctcgcgc gcatggaccc ggacctgcag
    36001 gaggccgacg cgctgatgat cgccgccgac caggcgtgct acgccgccaa gcacggcggc
    36061 cgtggtcacg tggtggagta tccgctgcca ttcgtcgccc cggggagcgc gcgcccgggc
    36121 tagcagctgg gcacgatcgt ccgccgtgac cggcagcgcg ctcagtgcag cgctttcggc
    36181 accgccagca tgaaggccgg aatctcggcc tcgaactcgt ggccgtcgtc cgcgcgcatg
    36241 cggtagctgc cctgcatcgt ccccaccggc gtggcgagca cgcagccgct ggtgtagcgg
    36301 aaggcctggc cggggccgag caccggctgc tcgccgacca cgccctggcc gtggacttcc
    36361 tgcatcttgc cgttgccgtc ggtgatcacc cagtggcgcg cgagcagctg ggcgcgcacc
    36421 tcgccggtgt tgaggacggt gatgtggtag gcgaacacga agtgctcgtc ctgcgggcgc
    36481 gactggtcgg cgacgtattc ggcgaccgcg gtcacctgga cgcggtaagg cgtggtctcg
    36541 ctcatcgtct tttgttctcg gtggggaaga ggggcaggcg ttaaaatagc agtttttccc
    36601 ggaagctccc gccatgtcga acgccgcccc gtccccgctc accgccctgt ctccgctcga
    36661 tggtcgctac catggcaagg tcgccggcct gcgcgaacac ttttcggagc acggcctgat
    36721 tcgcaaccgc gtgcgcgtcg aggtcgaatg gctgaaggca ctcgccgccg agccggcact
    36781 ggccgagatt gccccgttct cgccggccac ggtggtggag ctcgacgccg tggtggcgca
    36841 gttctcgacc gacgacggcg aagcggtgaa ggcgatcgag gccaccacca accacgacgt
    36901 caaggccatg gagtactggc tcaagaagcg cctcggccac aacgccgagg tgatgaaggt
    36961 gtccgagttc atccacttcg cctgcacctc cgaagacatc aacaacacct cgcacgcgct
    37021 gatgctggcc gaaggccgcg accgtgtgct gctgccggcg ctcgacgcgg tgatcgcccg
    37081 gttccgcgag ttggcccacg cgctcgccga cctgccgatg ctgtcgcgca cccacggcca
    37141 gcccgccagc ccgaccacgc tcggcaagga gatggccaac atcgcggcgc gcctgatgcg
    37201 ggcgcgcgca gccatcgccg gcgtggcgat gaccgccaag ttcaacggcg cggtcggcaa
    37261 ctacaacgcc cacctgtcgg cttggccgca attcgactgg gagggcttca accgccgctt
    37321 catcgagtcc ctggggctga ccttcaacga atacaccatc cagatcgagc cgcacgacgc
    37381 catggccgag ctcttcgacg ccatcgcgcg cgccaacacc atgctgatcg acgcctgccg
    37441 cgacatctgg atgtacatct cgctcggcta cttcaagcag aagctcaagg aaggcgaggt
    37501 cggctcctcg accatgccgc acaaggtcaa cccgatcgac ttcgagaatg ccgagggcaa
    37561 cttcggcctc gccaacgcgg tgctcaagca cttctccgag aagctgccga tctcgcgcat
    37621 gcagcgcgac ctcaccgact cgaccgtgct gcgcaacatg ggcgtgggct tcggccacac
    37681 ggtgctcgca ctcgacagtt gcctgcgcgg cctgggcaag ctcgaggccg agcccacgcg
    37741 cctggcggcg gacctggatg actgctggga ggtgctcgcc gagccggtgc agaccgtgat
    37801 gcgccgctat ggcatcgcca acccctacga gcagctcaag gccatgacgc gcggcaaggg
    37861 catcacccgc gaggcgctgc aggaattcat ccgcaccctg gccatccccg ccgaggcgcg
    37921 tgaccacctg ctggcgatga cgccggcgag ctacgtcggc aaggcggcgg agctcgcccg
    37981 ccgcatctga gcgtcgcccg aatccgccgg ccgcgcgccg gcgcccatcc cgacgccacc
    38041 cttgcggtgg cgttttcaat ccaggagaaa ccctatggcc ttcgccgaca acctcaagac
    38101 cctgcccggc gtgtctcacc tcgccgcgtt gaatctgatc gacgccgccg acgcggtcgt
    38161 cgccaccatc gagaacaagc ccggccaggc cggctcgctg gcggtctaca accacctcgc
    38221 ccagctctac ggcgcgatca gccccgaggc ggcgaagaag gggctggagc tctatgccga
    38281 gcacaccgag gatgcccgcg ccaaccccgg caagcatccc aacatcgacc gcctgatcgg
    38341 gctgatcgag cgtggggaaa cgctgcgggt gaagcaggtg ttcgcggtgt gaggttctgc
    38401 atggtgtgat cgcctgcgcg gcggcggttc agccgcgggg gcggcgacgc aaactcgtcg
    38461 ctgcgctcct cgggcatgcg tcgccttgtt tcccccgcga cgccctccac ctcggcgctc
    38521 ctgaagttgg cgcccgccgc cccgagcccc gacccgcagc cttgcgttcg ggggtgtcgc
    38581 aggctggcgt tttccagttg cgccgcgttc gcagggacgg aggcgttgcc gaggcgctga
    38641 ttcccacagg caagaaaaaa gcgccgcgcg ggcgcctttt ccggtcggga agcgcgcagc
    38701 agcctcagac caccgcctct tccttctcct tcttcggctt gatgaactgc tcgcgcgaca
    38761 cgcccagcca catcaccagc gggctcgcca ccagcaccga ggagtagatg ccgaagcaga
    38821 tgccgatcgt cagcgcgagc gagaagtagt acagcgtttc gccgccgaag acgagcatcg
    38881 acagtaccat cacctgcgtg ctgccgtggg tgattacggt gcgcgagatg gtgctcgtga
    38941 tcgcgtggtc gagcacctgt ggcgtggtca tgttgcgctt cttcttgaag gtctcgcgca
    39001 cgcggtcgaa caccacgacc gactcgttca ccgagtagcc cagcaccgcc agcaccgccg
    39061 ccagcaccgg gagcgagaac tcccactgga agaaggcgaa gaagcccagg atgatgatca
    39121 cgtcgtgcag gttggcgatg atcgccgaca ccgcgaagcg ccactcgaag cgcagcgcca
    39181 ggtagatcat gatgccgacg atcaccagca gcagcgccat cgagccgtcg gcggcgagct
    39241 ccttgccgac ctgcgggccg acgaattcca cccggcgcag ctcgggggcg gggccgccga
    39301 cctgctgcag cgtgttcatc accgtctcgg acacgcgcga tgtctccagg tcctcgcggt
    39361 tgggcaggcg gatcagcagg tcgcgtgcgc tgccgaagtt ctgcacctgc gcgtcggggt
    39421 agccgctgcc cgccagcgcc tcgcgaatcg gctcgagctg cggtgcctgg gcgtagctga
    39481 cctcgaccaa ggtgccgccg gtgaactcca ccgacaggtg caggccgcgg gtgaggagga
    39541 agaagaccgc cagcgcgaag gtgagcaacg agatgacgtt gaacaccagc gcgtggcgca
    39601 tgaaggggat gtctttcttg atgcggaaga attccatggt cgtgtttcct gtgcgttcgt
    39661 ccgcttattt cgtatccggc ttccacacct ggccgatcga caggctttcg accttgcggc
    39721 gacggccgta gatgaaattg atcagcatcc gcgacaccag gatcgcgctg aacatcgagg
    39781 tcaggatgcc caggcagtgc accaccgcga agccgcgcac cggaccggag ccgaacacca
    39841 gcagcgcgat gcccgcgatc agcgtggtga tgttggagtc gaggatggtg tcccaggcgc
    39901 ggtcgtaacc ggccgcgatg gccgcctgcg ggctggcgcc gttgcgcagt tcctcgcgga
    39961 tgcgctcgtt gatcagcacg ttggagtcga tcgccatgcc cagggtcagc gccatcgcgg
    40021 cgatgccggg cagggtcagc gtggcctgca gcagcgacag cagcgccacc agcagcagca
    40081 ggttggcggc gagcgcgatc gacgaaacga ggcccaccag caggtagtag ccgcacatga
    40141 acacgacgat ggcgaggaag ccccacagcg tcgagttgaa gcccttcgcg atgttctccg
    40201 cgcccaggct ggggccgacg gtgcgctcct cgatgatctc catcggcgcc gccagcgagc
    40261 cggcgcgcag cagcagcgcg acgtcgttgg cctcgacggt gctcatgcga ccggagatct
    40321 gcacgcgccc gccgccgatc tcgctgcgga tcaccggcgc ggtcacgacc tcgcccttgc
    40381 ccttctcgaa cagcaggatg gccatgcgct tgccgacgct ctcgcgcgtg acgtcgcgga
    40441 agatgcgcgc gccggcggca tcgagcgtga ggtgtaccgc gggttcgttg gtctggccgt
    40501 cgaagccggg ctgggcgtcg gtgaggcggt ctccggtgag cacgacctgc ttcttgacca
    40561 gcagcggcga gccgccacgc tcgttgtaga gctccgtgcc gaagggcacg ctgccggcga
    40621 gcgcctgctc gatcgcgccc ggggtgtcgt ccaccatgcg cacttccagc gtggcggtgc
    40681 ggccgaggat gtccttggcc ttggccacgt cctgcacgcc gggcagctgc acgacgatgc
    40741 gctcggcgcc ctgctgctgg atcaccggct cggcgacgcc gagctcgttg atgcggttgt
    40801 gcagggtggt gatgttctgc ttgatcgcga agtcgcgcat ggtctgctgc gcctgcggcg
    40861 tcagcgtggc gaccagcttg aggtcgtcgg cggcatcgtc gcgctcgacg agctgcaggt
    40921 cggtggtgct gtcgaggatc gcgcgccggc ccgcctcgcg ctgcgcggcg tcgcggaagc
    40981 ggatcaccac ggtgttgcct tcgcgggtga tgccggcgtg gcgcacgctc ttgtcgcgca
    41041 tcagggtgcg caggtcgccg gtggtcgagt cgagacgctt gaccagcgcg cccggcatgt
    41101 ccacctcgag caggaagtgc accccgccgc gcaggtcgag gccgagatac atcggcagcg
    41161 cctggatcga ggtgagccag ctcggcgagg ccgacagcag gttgagcgcg accacgtagg
    41221 acgggtcggc ggcgtcgggg ttgaaggtct gctcgatgac gtccttggcg cgcagctggg
    41281 tctcggtgtc cttgaagcgg gcacgtacgc tggtggcgtc ggagaagatg ccggtgttct
    41341 cgatgccggc cgccttgagc gcaccggcga tgcggtcggt ggtggccgcg gtgtcgagct
    41401 tgagcgtggc cttggcgctg gacacctgca ccgccggcac ctcgccgtag aagttgggca
    41461 gggtgtagat caggccccag agcagcacga ggccgatcag gaggttcttc cagaggggat
    41521 agcggttcat cgtcggcggg aatttgggtg gagcggggcc cgacgggagg cgtcgggctg
    41581 cgcctgccgg gcgcctggcc gcacccgtgg gggcggcgac ggattgcagg ggccggccat
    41641 gggcccgcgg cggcggcgcg gtcgagccgc ccgccaccgc gccgggaggg tcagatcgcc
    41701 ttcagcgtgc ccttgggcag cacggtggcg acggcctgtt tctgcgcggc gaccacggtg
    41761 ttgggggcga cttcgatctg cacgaagctg tcgccgactt cgacgacgcg gccggcgatg
    41821 ccgccgccgg tgacgacttc gtcacccttg gcgagcgcct cgaccatggc cttgtgttcc
    41881 ttcgcgcgct tcatctgcgg gcggatcatc aggaaccaca gcaccacgaa catcaggatc
    41941 agcggcagca ggcccatcag gcctccggtg gggtcggcgg cgccagcggc ctgggcgtag
    42001 gcattggaaa tcagcacgtt gaaactccgg ttggaagaaa aacgatgaag tatagcagca
    42061 gccgggacgg ggcccgggag gggtgagacg gcccgcgcgg cgaagggttc agcccgtgcc
    42121 ggtggcgcgc tcgccgcgga agcgctgtac gtagggcgcg aactcgccgg ccgcgatcgc
    42181 ggcgcgcagc tcggctgtaa gcgtctggta gtagcgcagg ttgtgcaccg tgttgagcat
    42241 gctgccgagg atctcgccgg tgcggtgcag gtggtgcagg taggcgcggc tgaagttgcg
    42301 gcaggtgtag cagtcgcagc ttgggtcgag cgggcgggtg tcggccttgt gcaccgcgtt
    42361 cttgatcttg atgtcgccgt agcgggtgaa cagccagccg ttgcgggcgt tgcgggtggg
    42421 catcacgcag tcgaacatgt cgatgccggc ggcgacgccc gcgacgatgt cctcgggcgt
    42481 gcccacgccc atcaggtagc gcggcttgcc ctgcggcagg cgcggcgcag tgtgggcgag
    42541 gatgcgcgcc atgtcgtcct tgggttcgcc caccgacagg ccgccgatgg cgaagccgtg
    42601 gaagccgatg tcttccagcg ccgcctgtga ttcgtcgcgc aggtcctcgt acatgccgcc
    42661 ctggacgatg ccgaagaggg cgttcttgtt ctcgaggcga tcgaactcgt cgcgcgagcg
    42721 cttcgcccag cgctgcgaca ggcgcatcga cttggccgcc tcgtcgcgag tggcggggta
    42781 gggcgtgcat tcgtcgaaga tcatcacgat gtccgagttc agcacgctct ggatctgcat
    42841 cgagatctcg ggcgtcagga agagtttcgc gccgtcgatc gggctggcga acttcacgcc
    42901 ttcctcgctg atcttgcgca gcgcgcccag gctgaagacc tggaagccac ccgagtcggt
    42961 gaggatgggc ttgtcccagg ccatgaagcg atgcaggccc tggtgcgcgg agatgatgtc
    43021 cagccccggg cgcagccaca ggtggaaggt gttgcccagg cagatctgcg cgccgatgtc
    43081 ggagagcatg gccggcgtca tcgccttgac cgtgccatag gtgccgaccg gcatgaagac
    43141 cggggtctcg accacgccgt gggcgagggt gaggcgtccg cggcgggcgc cgccgtcggt
    43201 ggtgaggagt tcaaactgca tcgtcgttct tgtcgttctc ggattcgttg cggcgggtga
    43261 tgaacatcgc gtcgccgtag ctgaagaagc ggtagcgctg ctcgaccgca tgcgcatagg
    43321 cgcggcggat gctgtccatg ccggcgaagg ccgacaccag catcatcagg gtggatctgg
    43381 gcaagtggaa gttggtgacc agcgcatcca ccacctggaa gcggaagccg ggcaggatga
    43441 agatctcggt ctcgccgctg ccggcttcga gcgcgccctc ctgcgccgcg gcctcgagcg
    43501 cgcgcatgct ggtggtgccg accgcgatca cgcgcccgcc ggcggcgcgg gtggcggcga
    43561 tggcgtccac cgtctcctgc gggatcacgt agcgctcgcg gtgcatgcgg tgctcgccga
    43621 gatcgtcgac gcgcaccggc tggaaggtgc cggcgcccac gtgcagggtg agccaggcgc
    43681 acttggcgcc gcgcgcggcg atgcgcgcga gcagcgcgtc gtcgaagtgc aggccggcgg
    43741 tgggcgcggc gaccgagccg ggcgcgcgtg catagaccgt ctggtaacgg gactcgtcgg
    43801 cctcgcctgc ggcgcgctgg atgtagggcg gcagcggcag cttgccgtgg cgctcgagca
    43861 gcgcgaggag gtcttcgtcg ggcggaaagc gcaggtggaa gaactcgccc acgcggccga
    43921 gcacctcgac ctcgaacgcg tccgccaggc gcagccggct gccggtcttc ggcgacttgc
    43981 tcgcccgcac ctgggcgagc gcctcgtgcg ggccgaccgg ccgctcgatc agcacctcga
    44041 cctggccgcc gctgtccttg acgccgtgca gacgggcatg gatgacgcgg gtgtcgttga
    44101 acaccagcag atcgccggga ttcacgcgct ccgcaagggc ggaaaacacc gcgtcggtga
    44161 gccgcggcgc ggcgccctcc tgcggctcca gcacgagcag gcggctggcg ctgcgctcgg
    44221 ccagcggtgc ctgggcgatc agctcgggag gcagggtgta gtcgaagtcg gcgagggtga
    44281 gggacatcgt gtcgtgttcg gtggagattg ttctggtccg gagcggctga cggctcgctt
    44341 gccgccccgc ctcgtgttcg gggataatgc gcgtcccgtg ccgggatggc ggaatcggta
    44401 gacgcagcgg attcaaaatc cgccgccgca aggtgtgtgg gttcgagtcc cactctcggc
    44461 accagttttc ggctccgccg gtcggcatcg accgccggcg gctcttcgat gaacgcggcg
    44521 ttcgaccccg cccgtcgagt agtcgttcgc gacggagtcc tccgctcgct tttcctcttc
    44581 ctgcgttccg gctgcctgcc ttcttgctga gggagcgcgc ttgccagcgt tgccggtcgc
    44641 cgtccgttcc cggcccgcga ttctgtagcg ccccggggcg aaaatcaaat caggcgaagt
    44701 tctgcttcgc gtgcgcgccc tcgctgatcg cgcgcaccgc ggggtgggtc agtctgcgct
    44761 ccaccgagat cgcgtagtag gtttcggtga cgcccttcgc gtcccccaga tgcaccagcc
    44821 cgccctggcg gcagagctgc tcgccgacca gcgaagcgct cgggaagatg cccgcgccgg
    44881 cctcggcgaa ggcgcgcatc agcgcgctgt cgtcgaattc gccgacgatg cgcgggcgca
    44941 gcccggcggc ctcgatccag cgcagcagcg gcccgcgcac gctcgcctcc accccgggca
    45001 gcagcagcgg tgcgccgtcg aggcgggcgg ggaagtcgcc ggcaaggcgg gcggcgagcg
    45061 cgggcgcggc gtagaagccg atggtcgatt cgccgagcgc gtggctgaaa ccgcgcacgt
    45121 ccatggcggg cggcagcggg ctgtcggcga gcaccacgtc gagcttgtgg atcgcgagtt
    45181 cgccgagcag ggcgtcgaac ttgccctccc ggcagacgat gcgcatcggc tcggggagat
    45241 ccatcgaggg tgccagcagc aaccacgcga tcgccttcgg caccacgtcg gcgatgccca
    45301 cccggaaagg caccgcgcgc cccgtcgcgc ggctctgcag cgcttcctcg agctcgttgc
    45361 cgaggcggaa gatctcctcg gcgtagtcca gcaccatgcg cccggtctcg gtcagcacca
    45421 ggccgcggcc ctggcgcttg aacaggttta cgccgagatc ggtctccagg gtggcgatct
    45481 ggccggacag ggtctgcggc gccaggccgg aacgttccgc cgcgcgcacg atcccacccg
    45541 cgcgggccac cgtccagaaa tgatggagct gcttgtagtt cagcatgggg cgcctccggc
    45601 gtgcgcactt cgatacttcg gaaaaatcgg attcaaattc tgctgaaccg agattctatc
    45661 gaacagtctg agcggtagta tccaccggag cacagggccc gatggatccg tgcaacgaaa
    45721 acgaaggaga ctggacatgc gtatcgatct gcattgcgac ggtgtggaga ccgcccccgg
    45781 cctgcgcgac tacgtggcgc agcggatgcg tgcttcgatt ggccgcttcc gcgaccacat
    45841 ccagtgggcg cgcgtgaagg tcgccgatgt gaatggtgcg gagggcggcg ccgacaagcg
    45901 ttgcgtggtt cagctgcgcc tgcgcaacct ccccgatgtg gttttcgcca tcactcagct
    45961 cgacgtgcgc gcggcggtgg acgaggccgc cgaccgggtc gcccgcgtgc tcgcccagcg
    46021 cgtgcgccgc caccagcgcg ggccggcgat ggccgcgatc ccggcatgat tttctcgtag
    46081 ttcctgcagc aaacccccgg tcgtccgccc gttcggcgcc ggttccaaaa caaatcgacg
    46141 atcccactgg aggacctcca ccatggaaaa ccgcatcagt accctgggcc gggccgaagc
    46201 ctcggtcctg cagaccaaca aggtcatccg caacacctac atgctgctgt cgctcacgct
    46261 ggccttctcg gcgctgaccg ccggcctgtc gatggcgatg ggcgcgccgc gcctgggcat
    46321 cctgatcacg ctggccggct acttcggcct gctcttcgcc accaccaagt tccgcgacag
    46381 cagcctgggc atcgtgttcg tgttcgcgct gaccggcttc atgggcttca ccctcggccc
    46441 gatcatctcg tcctacctcg ccctgcccaa tggcggctcg atcgtgatgc aggcgatggc
    46501 cggcaccgcg gcgatcttcc tcggcctgtc ggcctacgcg gtgaccacca agaaggactt
    46561 ctccttcatg ggcggcttcc tgatggtcgg catcctggtg gccttccttg ccggcctggg
    46621 cgcgatcttc ttcgagatcc cggcgctgtc gctgaccgtc tcggcggcct tcgtgatgct
    46681 gatgtcgggc ctcatcctgt acgagaccag caacatcatc cacggcggcg agaccaacta
    46741 catcatggcc acggtgtcgc tgttcgtgtc gatcttcaac ctcttcacca gcctgctgca
    46801 cctgctgggg ttcgccaaca acgactgacc cggagcgccc gccacggcgg gcgccttccg
    46861 gaatggggcg gtggccgcga ggcgccgccc cattcttttg cgcgcgagca cgggttttgc
    46921 gctgcagcac gcgcgccgcg cgcggccggg cggctggcgt tacaatggcg cacatttttg
    46981 actgggacag cccgggatga agaccacctt tcttgatttc gaaacggcga tctccgaact
    47041 cgaggaaaag atcgaccagc tgcgcttcgt ccaggacgat ccggcggtcg acatctccga
    47101 cgagatcaag cggctcgagg ccaagagcca gtcgctgacc aaggacctct acgccaagct
    47161 cagcccctgg cagatcgccc aggtcgcgcg ccacccgcag cgcccctaca cgctggacta
    47221 cgccagcctg atgttcaccg acttccacga gctgcacggc gaccgcaact acgccgacga
    47281 caaggccatc gtaggcgggc tggcgcgctt caacggccag gcctgcgtgg tgatcggtca
    47341 tcagaagggt cgcgacacca aggaaaagat cgcgcgcaac ttcggcatgc cgcgccccga
    47401 gggctaccgc aaggcgatgc gcctgatgaa gctggccgag aagttcgcgc tgccggtgtt
    47461 caccttcgtc gacacgccgg gggcctatcc cggcatcggc gccgaggagc gcggccagtc
    47521 cgaggcgatc gggcacagca tctacctgat gtccgagctc aagaccccga tcatcagcac
    47581 catcatcggc gaaggcggct cgggcggcgc gctcgcgatc gcggtcgccg accgcgtgct
    47641 gatgctgcaa tactcgacct actcggtgat ctcgcccgaa ggttgcgcct cgatcctgtg
    47701 gaagagcgcg gagaaggccg ccgacgcggc cgagaccatg ggcatcaccg cggcgcgcct
    47761 gaagtcgctc gagctggtcg acaaggtggt cgacgagccg atcggtggcg cccaccgcga
    47821 ccaccgtgcg atggccgcca cgctcaagcg cgcgctcgcc gacgcgctgc gcgagctcga
    47881 ggcgctgtcg ccctccgagc tggtcgagca gcgctaccag aagctgatga gctacggccg
    47941 ctacaaggag aaggcggcct gaggccttct cgccgctcga gcccggtcgg gcgcgcgctg
    48001 ctgcgcctgc gctcgcgact ccatcgatga ggaccccgcc cgccgacgtg ctgcccgaat
    48061 ccgccgccgc ggtcctcgcg gctgccggtg tcggcgccgg cagtcgcgtg tgctgcgcgc
    48121 tctccggcgg ggtcgattcg gtggtgctgt tcgagctcct gcgccgcctg cagccgcgct
    48181 tcggcttcag cctgcgcgcc gcccacgtcg accacggcct cagcccgaat gccgccgcct
    48241 gggcgcgcgc ctgcgccgag cgctgcttgg ccgcgggtgt tcccctgcaa gtcttccgcg
    48301 tcgaagtcga gcgcgcccac gccgacggcc tcgaggccgc cgcgcgcgcc gcgcgccacg
    48361 ccgcgctcgc ccaagtggac tgcgactggc tcgccttcgg ccatcaccag gacgaccagg
    48421 ccgagaccct gctgttccgc ctgctgcgcg gcacgggcgt gcgcggggcg gcggcgatgc
    48481 gcgcggtgga gccgggcggg gcagggcggg cggggcggct gcgcccgctg ctcgggctgc
    48541 gccgtgcgca gctgctcgag tgggcgggcg cgcacggact cggttgggtg gaggacgaga
    48601 gcaacgccga gctgcgcttc acccgcaacg ccctccgcca tcgtctgctg ccggtggccg
    48661 aaggcctctt cccggcggcg gtgccgaacc tggcgcgcgc tgccgcgcat ttcgccgagg
    48721 ccgacgcgct gctcggcgaa ctcgccgcga tcgatgccgc ggcctgcggc gcgcccgtgc
    48781 tgtgccgcgc ggacttgctc gccctgacgc cggcgcgcca ggccaacctg ctgcgctggc
    48841 tgctgctcgg gtccggtgcg cccttgcccg gcgcggcgcc gctcggcgaa gccctgcgcc
    48901 agctgcgcgc gtgcgatccc gcccaccccc tgcgtctgcc gctcggcgcc tgggtgtgct
    48961 gcgcctaccg cgatcgtgtg tggctggagc cggcgcagcc gcccgctccg caggcgctgc
    49021 actggcaggg cgaggcggcg atgcactggg gcggcgatga agtacgcctc gtggcgtcga
    49081 tcggcgcggg actgggcagg ggctgcctcg aagatgccgg cgaggtcctg ctcgggcccc
    49141 ggcgcgaggg tctgcgcatg cgactgcacc ccgcgcggcc gcggcgcgag ctgcgcaagc
    49201 tctgccagga ggccgcgatt cccgcgtggc tgcgcgaccg cctgccggtg ctgtgggtgg
    49261 acggcgagcc cgcgtgggtg ggcggcatcg gcgtggcggc ggagttcgcc tgcgcgccgg
    49321 gggcagcggg gctcgtgccg gagtggcgac cgggcggcgt cacccccgcg ccgccaccct
    49381 gagcgctgaa cggtggcgcc cgcgctcagg tgcgcagcgt ggtcagcagc tgtgccagct
    49441 cgaccgccga gcgcacctcc atcttctcga agatgcgcga gcggtgcgcc tccacggtgc
    49501 gcatcgagat cgacaggtcg tcggcgatca ccttgttgta cttgccttcg aggatcagct
    49561 ccatgacctc gcgctcgcgc tgggtgagcg tggccaggcg tgcctcgatg gtctcgcggc
    49621 tggcggcggc gcgcaggcgc tcgtcgtcgg tggcgagcgc cttctcgaca aggtcgacga
    49681 gatcgtggtc gttgaagggc ttctcgatga agtggaaggc gccctttttg agcgccgcca
    49741 ccgccatcgg cacgtcgccg tggccggtga tgaagatcac cggcagctgg cagccgcgcg
    49801 cgagcagctc gtcgaagcac tccagcccgc tcatgccctg catgcggatg tcgagcacga
    49861 tgcagccgcg ccagtcgccg cgccagtcgg cgaggaaggc ctcggccgag gcccagctcc
    49921 ggcagggtac gccgcgcgtc ttgaacagcc attcgagggc gtcgcggatg gcttcgtcgt
    49981 cgtcgatgat gtgcgtgcag gcgggattca ttcgaactcg gcgtccaccg ggagggaaag
    50041 ggtgaagatg gtaccgccgc ccggccgcga ttcgaaggcc aggcggccgc ggtgcagctc
    50101 ggcgatcgag cggcagatgt tgagtcccat gcccatgccc tcctgcttgg tggtgaagaa
    50161 gggctcgaat agccgcgcgg cggtttcggc gggaatgcct gcgccgcggt cggcgacggt
    50221 gacggtgacg aagcggccct cgcgcgcggt gtgcacggcg acgacgcgct cggctggcgg
    50281 ggtgtcgcgc atcgagtcca tcgcgttgcg caccaggttg acgacgatct gctcgatcat
    50341 caccgcgtcc gccggcacct gcggcaggcc gtcggcgagc tcgctgcgga tccgcatgcc
    50401 gcgcttgcgc gcgtcggcct cgatcaggcc gatggcgtcg cggatcaccg cggcgaggtc
    50461 gagcgcctcg cgcttgggtt cggagcggcg cacgaaatcg tgcacccggc ggatgatctc
    50521 gccggcgcgc tgggcctgac gggcgatgcg ctggtgcacg tcgagcagct cgctgcggtc
    50581 gagcggcgcc tcgtcctgca ggcggttgat gcagcccgtg gtgtagcttg cgatcgccgc
    50641 gagcggctgg ttgagctcgt gcgcgagcgt ggacgccatc tcgcccatgg tgatcaggcg
    50701 cgagctctgc tgcaggcgct cctcctgctg gcgggcctgt tcgcgcgcgc gcttctgttc
    50761 ggtgatgtcg agcaccgaac ccatccagcc ggcgtggcgg ccggaagcgt cgatcagcgg
    50821 cgcctcgaag acgaggacgt cgagggtttc gccgttcttg cgccgcaggc gcacctcggt
    50881 gccttcgctg gcgctgccgc tggtcatgat ctggcggtgc agctcgaagg tctgctccag
    50941 cctttccggg tcccagtagg gcatcggcgg cttgcgaccg agcagctcgt ccgcgctgaa
    51001 gccggtcatg cggcaaaagg cggagttgac gtaggtgagg cggccttcga ggtcgcgcgc
    51061 acgcatgccg gtcagcatcg agtcctccat cgccttgcgg aagaccgact cggcgcgcag
    51121 cgccacctcg gcctgctgac ggcgtgcgag ctggcggcgc agttgcatca cgctccacac
    51181 gatgatgccg gccagcagca cgatcgaggc gccgagcagc accgggatcc agcgcgggtc
    51241 ggagcggtag gcggagatct gcagcgtgag gccgtgtccg ggcggatcga agggcatcga
    51301 ataggacagc cgcggcgaca ccggcgcgac tttcgacttg gcggcgatcg tgcgtccgtc
    51361 gctgtcgagc acggcgacgt ggtagcgctc ggagaaccac cacggcagct cgcgcatcac
    51421 cacgtccgag agcgcataca cgccgaccac catgccgacc tgcgcctcgt ccgaccacac
    51481 cggcacgtgc acctcgaagt ggtgggtgcc accgggcgcc ggatgcgcgg ggccatacac
    51541 cgggcggccg atggcgcgtg ccagccgcac gctggattcg tcgaccggca ccgcggcagc
    51601 atccgcggag gcgtcgtgca ccgcctgggc gggagccagc ggcggcatcg cgccgcgcac
    51661 ttcgccttcc ggcgacagcc acaggatgcg cgcgaggccg cgctcggatg tcagcaggct
    51721 ggcgagccga gcctcggtct gcgccgacag cgtgggtgtg gcgagcagct cggggccgat
    51781 ctcctgcagg tggttctcgt tgcggtccag gttgaagcgc aggttctgct ccatccacag
    51841 cacgtcgctg atcagcgtcg cgcgctgggc ctcgtcgtcg tactcgcggg tgagccacac
    51901 cagcgcggcg atcgcggcga ggaagagcgc caggctgagg tagggcagct gctgcagcca
    51961 gcgccggcgc gccgggggcg ggagagggtc ggagaggctc atcggaaaag gctgcgggtc
    52021 gggatccggc ggcttgcggt tgtcacgcat gcggagatcc acaatagcag ccctcacagg
    52081 gcgaaacgat actgatcccc gacacagcga aacttatcgc cgtgcatgcg cccggtaagg
    52141 agctgtccgg gcggcgaaaa ctacctag
//
LOCUS       DAOQBS010000007        51771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_288_length_51771_cov_15.049346, whole genome shotgun sequence.
ACCESSION   DAOQBS010000007 DAOQBS010000000
VERSION     DAOQBS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 51771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51771
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_288_length_51771_cov_15.049346"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="PKN25_01655"
     CDS             <1..168
                     /locus_tag="PKN25_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNV89629.1"
                     /translation="LKKLDKVAYIRFASVYRNFADVDEFAEVIREVQTRPRRGKADKG
                     GADPGHDLFGG"
     gene            149..1246
                     /gene="ribD"
                     /locus_tag="PKN25_01660"
     CDS             149..1246
                     /gene="ribD"
                     /locus_tag="PKN25_01660"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNV89630.1"
                     /translation="MTFSAADHAAMARALQLAARGLDTTSPNPRVGCVIVRDGTTVGE
                     GWHARAGEAHAEVHALRQAGAAARGATAYVTLEPCSHFGRTPPCADALIEAGVARVIA
                     AMEDPNPLVAGRGLARLRASGIATAHGLLAEAAQELNIGFVSRMTRGRPWVRLKVAGT
                     LDGKSALDNGVSQWITGPAARRDGHRWRARACAILTGIGTVREDDPQLGVRDVPCERQ
                     PLRILVDARLEVSPQARILQGEPILIATGSTDQARIDMLRAAGHEIVFVPNAAGKVDL
                     GALLRLLGERGINELHVEAGFKLNGSLLREGCVDELLIYLAPMLVGDAAQGLFNLPAL
                     TRLEGATRLDIRDLRRIGPDLRLIARPLAQG"
     gene            complement(1232..2014)
                     /gene="moeB"
                     /locus_tag="PKN25_01665"
     CDS             complement(1232..2014)
                     /gene="moeB"
                     /locus_tag="PKN25_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNV89631.1"
                     /translation="MNDDQLLRYSRHILLPEIDVEGQQALLDARALVVGAGGLGSPAA
                     MYLAAAGVGTLVLADHDTVDLTNLQRQIMHSADMVGLPKVASARATLHRLNPQTRLEP
                     IQARLEGEALEAEVGRADVVLDCTDNFATRHAINRACVKHRKPLVSGAAIRFDGQVSV
                     FDLRKPDSGCYHCLFPEGEDIEEVRCAVMGVFAPIVGIVGTIQAAEALKVLIGCGRTL
                     DGRLLLLDGLSMEWRSVALGRDPGCAVCGTARCAGAVESALR"
     gene            complement(2022..2471)
                     /locus_tag="PKN25_01670"
     CDS             complement(2022..2471)
                     /locus_tag="PKN25_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89632.1"
                     /translation="MRRETAYKLAGRHHDRPLPGADIHKHREIRFKPLPPGQVERAWK
                     SLQLLKGLQLERTDDPLGIVVRYSVLDYSLESLEDALREAGFALENSLYIKLVRALVY
                     FSEETQRHNLLSPERLIKQSNEVYIQAWDHHVHGDHDDTPLELREYK"
     gene            complement(2499..3950)
                     /locus_tag="PKN25_01675"
     CDS             complement(2499..3950)
                     /locus_tag="PKN25_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNV89633.1"
                     /translation="MRSSKLKQFGLVMTGMVAGIMISLNFSANADRSAPLPLPVDELR
                     AYADVFNAIKQGYVEPVEDKKLITDSISGMLSGLDPHSAYLDAEAFKDLQVGTQGEFG
                     GLGIEVGMEDGFVKVISPIEDTPAFRAGVLAGDLIVKLDDTPVKGMSLNDAVKRMRGK
                     PRTDIILTIMRKGQSQPIVIKLTREVIKVQSVKSKVVEPGYAYLRIAQFQENTAASVV
                     EHLAKLGAQGPVQGLVLDLRNDPGGLLHGAVGVSAAFLPEETLVVSTDGRTEDAKREY
                     RAIQADYLRGTRDDFLRRLPAYARDVPMVVLVNAGSASASEIVAGALQDHKRARVIGT
                     QTFGKGSVQTILPLNNNTAIKLTTARYYTPSGRSIQAKGIAPDIVVEETANGSGRRVR
                     EADLLGHLENDKGAVEAAKAAAGKADGAPAEETAAETARFELAGDNDHQLKQAIRLLK
                     GEDIAHAAPAVEAEAAGGQAAAAGAAQPAAAKQ"
     gene            complement(3995..>4555)
                     /locus_tag="PKN25_01680"
     CDS             complement(3995..>4555)
                     /locus_tag="PKN25_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNV89634.1"
                     /translation="RAEHEQRGETAARAEPAPVPRLEAPPPARVERAQRPEPVVGEVR
                     DAASATPTGVRFNQLRGQLRFPVRGELVGRFGAPRAEGGTTWKGVFIRAGNGAEVRSV
                     AGGEVVFSDWLRGYGNLIIVDHGSDYLTIYGNNDSLLKEVGDRVGGGEPIASVGAAGV
                     GNDSGLYFEIRHQGQPLDPMQWMRLN"
     assembly_gap    4556..4655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4656..5595)
                     /locus_tag="PKN25_01685"
     CDS             complement(<4656..5595)
                     /locus_tag="PKN25_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HNV89635.1"
                     /translation="MSPRSLKSPIAGACIAALAALLVVPGPAFAQAGGEIAERRSDLD
                     ELKQRIRELQEEMAKAEADRTSAQKALVDSERSVSRAVRELARIEGERRNAEKRLAVL
                     EGEQRETAARIDARRGELADWVRRHYVHGAADGVAPLLSARDPNQLARDAHYLEHLGR
                     ARLELIEGLRTDLSETRRRADEISARRDRLATLESEQRDRQQQLQTEQSRRKAALAEV
                     SRELQAKKSEVDALQQDEQRLGKLIETLARRARLAAAREAARREAEQREAERRAAAQR
                     EAERREAAQREAERRAARARAEHEQRGETAARAEPAP"
     gene            5803..6132
                     /locus_tag="PKN25_01690"
     CDS             5803..6132
                     /locus_tag="PKN25_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNV89636.1"
                     /translation="MDKDAITTLIDKHEHIETAARALKAIAHPLRLKILCVLGSGEEC
                     VQDIVDAVGTSQSNISQHLAILRDKGVLQTRKDANRVYYRVGDERTLQLIVLMREVFC
                     ADDDAER"
     gene            6165..6581
                     /locus_tag="PKN25_01695"
     CDS             6165..6581
                     /locus_tag="PKN25_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV89637.1"
                     /translation="MEFLQHNWYWAALAAASGTWLMVELVRSRGDDTQLSPVEATLLV
                     NREDAIVIDVREQGEYAQGHIPNARHIPAGEIERRGKEMEKWKDHPVILCCTTGARSN
                     SAAGALRKAGFNRIYNLRGGMMEWQKAGQPVSRKRK"
     gene            6584..6853
                     /gene="grxC"
                     /locus_tag="PKN25_01700"
     CDS             6584..6853
                     /gene="grxC"
                     /locus_tag="PKN25_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNV89638.1"
                     /translation="MQPPIRMYATATCPYCIRAEQLLLDKGVVGLDKIRVDLDPSRRE
                     EMMKLSGRRTVPQIFIGDYHVGGCDDLLALERGGRLAALLRGESA"
     gene            6979..7443
                     /gene="secB"
                     /locus_tag="PKN25_01705"
     CDS             6979..7443
                     /gene="secB"
                     /locus_tag="PKN25_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNV89639.1"
                     /translation="MSENTQPVFSIEKLYVKDLSVEVPGAPQIFLERETPQVNVQLRT
                     EGQVVEEGVYEVTLTVTVNARIGEERNLFLVEVAQAGIFQIRNLPEGELEPVMMIGCP
                     NILFPYAREAVSDAVTRAGFQPVLLSPVNFESLYQAQRQQAIEQANGEVPIQ"
     gene            7517..7951
                     /locus_tag="PKN25_01710"
     CDS             7517..7951
                     /locus_tag="PKN25_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV89640.1"
                     /translation="MLAVSAPLGGAALPVAAQAIEYRSVAASTLLREQPAPDAEALFR
                     LRPGTPVEIVVREDGWMRVRDPAGGFGWVEGGALVTRRTVIVTAERAIVRRAAQETAA
                     PAFEATRNVVLELLEPASEGWARVRHVEGFEGYVHASEVWGL"
     gene            7948..8940
                     /locus_tag="PKN25_01715"
     CDS             7948..8940
                     /locus_tag="PKN25_01715"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNV89641.1"
                     /translation="MSRRRIAVFGAGAWGTALAQAFSASHEVVLWGRDRGQLEEIARI
                     HENTRYLRGVRLRDTLAVEADFARAAAQAELHLVVTPLAGLRTTVRELRRLAPGRALL
                     WACKGFEAGTGKLPHEIVAEECGAGSASGVLTGPSFAAEVARGLPAAIALAAHDADFA
                     RHWVGVLHQPRLRIYANTDLVGCEVGGAVKNVMAIAAGVSDGMGFGLNARAALITRGL
                     AEIARLARALGARGETLMGLAGMGDLILTCTGDLSRNRRVGLALAEGKGLADILRQLG
                     HVAEGVSTAREVVGLARRHGVEMPICEAIDALLHHGLDARGAVERLLARDPRAE"
     gene            complement(8962..9438)
                     /locus_tag="PKN25_01720"
     CDS             complement(8962..9438)
                     /locus_tag="PKN25_01720"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNV89642.1"
                     /translation="MFEIVLYQPEIPPNTGNVIRLAANTGCRLHLVAPLGFDLSDKQL
                     ARAGLDYHDLTCLTVHPDWAHCREVLAGRRMFAMSTRASRRHDRVDFQPGDALLFGPE
                     SRGLPEEILREFAPERRVRIPMRPTNRSVNLSNAVAVVVYEGWRQHDFAGAVAQGD"
     gene            complement(9576..10307)
                     /locus_tag="PKN25_01725"
     CDS             complement(9576..10307)
                     /locus_tag="PKN25_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNV89643.1"
                     /translation="MSNSLHPLRVLFNHAMDVLAPQDCFVCGATSGGEAVCAACADEL
                     PRRPSTACPQCGLPGLDGGRCGACRRSAPAFDATRAVFDFAFPVDAMIHALKYRHQLA
                     VGRYLGDALARCGADFAAEDGAGADLVLPMPLHPRRLAERGFNQAVELARGFARARDL
                     PLGLAVVRKLRDLPAQAGLDREARLRSPRGAFECVEDLSGRRVIVVDDVMTTGATLDE
                     LARCLKGRGASWVGNLVVARTPSPS"
     gene            10372..11352
                     /gene="bioB"
                     /locus_tag="PKN25_01730"
     CDS             10372..11352
                     /gene="bioB"
                     /locus_tag="PKN25_01730"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNV89644.1"
                     /translation="MTTTSATIERPAAPPSRHQWATAEVEALFELPLLELVFRAQQVH
                     RSHFDPQEVQRSTLLSIKTGGCSEDCGYCSQSARHDSGLARQRLLPVDEVLANARAAK
                     ARGASRFCMGAAWRGPKDQDLAPVLEMVREVKALGLQTCVTLGMLKEGQAEQLADAGL
                     DYYNHNLDTAPDFYGRVITTHTLQDRLDTLDKVRGAGINVCSGGIVGMGENRRQRAAL
                     IAQLANLPAPPESVPINNLVQVPGTPLAGAGALDPFEFVRTIAAARITMPSSYVRLSA
                     GRGEMSDELQALCFLAGANSMFYGERLLTTDNPDADRDDRLFARLGLRAI"
     gene            11364..12326
                     /locus_tag="PKN25_01735"
     CDS             11364..12326
                     /locus_tag="PKN25_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV89645.1"
                     /translation="MDTPTPGQDVSARDAPPGQAPAAPGFALDRSLLRRRFERAAPHC
                     DGADALAREVARRMDERLDYIRLSPQRVLDLGCGTGADLPRLAERFPAATLLAADFAP
                     AMLARARARLRPQGGGLLRRLIGGRPAAAPHFVADACALPLAHASLGLVWSNLMLPAL
                     DDPLPAFREVHRTLQVDGLFMFSTLGPDTLRELRAALPATAGERVHRFIDMHDLGDAL
                     VQAGFSDPVMDMEMLTLTYADLDGLFADLRASGGNNAASTRPRGLSGRTGWEAARAAY
                     ERLRHDGRLPASFEIIQGHAWKPAPKTTADGRAIMRFQPRPPAR"
     gene            12366..13016
                     /locus_tag="PKN25_01740"
     CDS             12366..13016
                     /locus_tag="PKN25_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HNV89646.1"
                     /translation="MSSRPVWLFDLDNTLHNASPHIFPHINRSMTRYLEQHLGLALDE
                     ANALRMQYWHRYGATMLGLMRHHGTDPDHFLVETHRFDRLHEMMVFERALRGMLRALP
                     GRRIVFSNAPRHYAEAVLEIMGVRRLFEDVVGIEDLDYHPKPGIRAYRGLLQRRRLNA
                     AQCIMLEDSAVNLRTAKRLGMRTVLVGGGLRTPAYVDLRIPNILALRRQLGRLQVR"
     gene            complement(13086..13583)
                     /locus_tag="PKN25_01745"
     CDS             complement(13086..13583)
                     /locus_tag="PKN25_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV89647.1"
                     /translation="MTARPVLRPLCAADLPDVLAIQRAAYGDGYQESAAVLGRKLELA
                     PQACWLAQADGAAVGYVFAHPWDDADAPPLHAPLRALPARAGHGFVHDLAVVPAARGL
                     GVAAALFGRVRDWSAAAGHRGLRLVALADAVPFWARQGFAALPAVLPAGYGEDACLME
                     RALRD"
     gene            complement(13583..14599)
                     /gene="hemB"
                     /locus_tag="PKN25_01750"
     CDS             complement(13583..14599)
                     /gene="hemB"
                     /locus_tag="PKN25_01750"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNV89648.1"
                     /translation="MRPTGAFPATRMRRMRRDEFSRRLMREHVLTANDLIYPVFVLDG
                     TDRSEAVPSMPGVERVSIDRLLGVAEEAMRLGVPALALFPVIDRAGKTPGAEEAWNPE
                     GLVPRTVQALKANFPDLGVITDVALDPYTSHGQDGLIDPADPRGYVMNDETLEALAKQ
                     ALCHAQAGADVVAPSDMMDGRIGRIRAELDGAGHIHTRILAYSAKYASSFYGPFRDAV
                     GSAGNLGKGNKHTYQMDPGNSDEALREVELDIAEGADMFMVKPGMPYLDIVRRVKQEL
                     QVPTYAYQVSGEYAMLKAAAANGWLDERACALEALLSFKRAGADGILTYFALDAARWL
                     KAGA"
     gene            complement(14886..15545)
                     /gene="yihA"
                     /locus_tag="PKN25_01755"
     CDS             complement(14886..15545)
                     /gene="yihA"
                     /locus_tag="PKN25_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNV89649.1"
                     /translation="MPLFRNATFEISIAKPSGLPPPSGAEIAFAGRSNAGKSSAINTL
                     AGHVRLAYVSKTPGRTQLINFFRLNNGALLVDLPGYGYAAVPEKIRRQWSGLIETYLR
                     TRESLIGLVLIMDSRHPLTPLDRNMIDWFAPSGRPMHVLLTKSDKLSRGAAAATLQEV
                     RRALEPLGPQVTVQLFSSLKKSGVEEVERVIGGWLVPAGTVGGDLADTPAGAVDAPPG
                     A"
     gene            15669..16298
                     /locus_tag="PKN25_01760"
     CDS             15669..16298
                     /locus_tag="PKN25_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV89650.1"
                     /translation="MIKRSLLLSSLLLVAGGLQAQDQAPDLEKAKQTAETVCAACHSA
                     DGNSQLPANPKLAGQHEDYLFKQLREFKGWNGNPPVRENAIMGAMVAGLEEADMRGLA
                     KYFATFEQKPEPAKSLGTIDLGQKIWRAGIPAKGVPACAACHGPAGAGMPAQYPRLSG
                     QFAEYTEAQMKAFRDGVRANDPNGMMRTIALKMTDPEIKAVADYATGLR"
     gene            16425..18509
                     /locus_tag="PKN25_01765"
     CDS             16425..18509
                     /locus_tag="PKN25_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNV89651.1"
                     /translation="MHSGIARALFELFSSMRFAISLLTILSIASVVGTVVRQNEPFNA
                     YLNQFGPFWFPVFEKLGLYSVYNAGWFLVILAFLVLSTTLCIVRQTAPMLREMRSFRE
                     RAREASLRSFAHRVSLVPALPPADAVERAQAYLAHAGFTSRVADNGEGTLLAARQGSA
                     GRIGYFLAHGAIVLICIGGLLDGNLPLHLQVWLGDKHTTAGDQVIANIPPSARLGTGN
                     PSFRGDVFIPEGRSTSFAVLGLADGILLQELPFNVTLDKFHIEHYQNGMPKRFASDIR
                     ITDRSSGQTTRHTIEVNHPLTVDGITLYQSSFEDGGSKLAIKARSLLPGRPGVLREIE
                     GVVGESLAFADGAAGFSYTLEFTDFRAFNVEDMRGNEGGQGDEGEAAPRGMDKLQRHL
                     GSGAKGAEVRDLRNIGPSFTFKLRDPAGQAREFHNYMLPIEQGGRWFLYTGLRDAQAD
                     AFRYMRIPLDERGGVDAWFAIRDRLLDPAQRDALVARFAARSFPTGRGQDAMREALVQ
                     SARQTLGLFAEKGYETLGGFIEKSVPEAERGPAADVLVRILQGLAWEAWMLHREAGGE
                     PPLQLDPARATFVNDTIAAISDSQFYGAPAWLQLDGFEERHATVLQATRSPGMPLVYL
                     GALLLVLGVFAMLYIRERRLFVLVKPNEALVAMSSNRRALDVDEAFRQHVEGLAAMLG
                     APVPASEPRESA"
     gene            18506..19690
                     /gene="ccsB"
                     /locus_tag="PKN25_01770"
     CDS             18506..19690
                     /gene="ccsB"
                     /locus_tag="PKN25_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HNV89652.1"
                     /translation="MMEIARPSPATPAGPAPAALRPHTPLLRRLGAFDWLYALALMLG
                     AAFALVRYHPVMDVYDDLILVLQVPVLAVIGWQWKPFRAFLAAVALLALASIALYQGD
                     LARAEQTFLLKYLISSQTAIMWMSALIFIATGAYWLGLLARSDFAERTGSALTWIAAV
                     MGTTGLFVRWYESYLLGPEIGHIPISNLYEVFVLFALMTALLYLYYEGRYATRRMGAF
                     VMIVISAAVAFLLWYTFTREAHEIQPLIPALQSYWMKVHVPTNFIGYGAFAVSAMVGT
                     AYLLAERGILASRLPPLRVLEDVMYKAIAIGFAFFTIATILGALWAAEAWGTYWQWDP
                     KETWALIVWLNYATWLHMRLTKGLRGAVLAWWAVVGLLVTTFAFIGVNMFLTGLHSYG
                     AL"
     gene            19740..20987
                     /locus_tag="PKN25_01775"
     CDS             19740..20987
                     /locus_tag="PKN25_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNV89653.1"
                     /translation="MNPNPAGGDDRSFLAVDAARAAILGALTPIAGRERVAVRAALGR
                     VLAEDVIAPCNVPAHDNSAMDGYAVRAADLSADGESALAVVGTAFAGRPFSGMVGAGQ
                     AVRIMTGATIPQGADTIVVQEVARREHERVFIPIGQKKGQNLRRAGEDLAEGGVAIPA
                     GKRCGPAELGLIASLGIAEVCVRRRLRVAVMSTGDEIASIGRPLGPGEVYDSNRYTLH
                     GVLTRLGCELLDLGVVPDRPEALEEAFREAAQAADVIITSGGVSVGEADFIREMMNRL
                     GEVAFWKLAIKPGRPMAFGRIGDAVLFGLPGNPVAVMVTFYQFVQDALLQLMGVAPLP
                     APERFPVRAAFSMRKQPGRVEYLRGRLQRSGEALAVAPAGAQGSGVLSSMSEADCFVV
                     LPADCTAVREGDTVLVQPFHGIV"
     gene            21044..21706
                     /gene="rpiA"
                     /locus_tag="PKN25_01780"
     CDS             21044..21706
                     /gene="rpiA"
                     /locus_tag="PKN25_01780"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNV89654.1"
                     /translation="MTQDELKKAAAYAALDHVVEGSIVGVGTGSTVNHFIDALAGMRD
                     RIRGAVSSSEASTQRLRAHGIEVFDLDGIESLPVYVDGADEIDGGFAMIKGGGGALTR
                     EKIVAAVSERFVCICDASKKVEVLGRFPLPIEVIPMARSHVARELRALGGEPRLREGF
                     VTDNGNLILDVHGLSITDPVTIEMRINQIVGVVTNGLFAVRGADVLLLAAPGGVQRLD
                     AR"
     gene            21796..22488
                     /gene="phoU"
                     /locus_tag="PKN25_01785"
     CDS             21796..22488
                     /gene="phoU"
                     /locus_tag="PKN25_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNV89655.1"
                     /translation="MTEHTLRQYDIELEEIRRGVLNMGGLAEAQVVKAIEGLRSGQLD
                     LLDTVIEGDKPINLAQVELDDDCSHIIAKRQPAAGDLRLVLAVIKIASDLERIGDEAK
                     KIAKAARRLHSAETPFVPRVGLTQAVELALELLRASLDAFARVNPEGIEDLRRKDAEI
                     DAGFKGVMRQLITYMMEDPRTISSCLEMLFIAKAIERVGDHAMNIAEHVVFVARGEDV
                     RFEKSQAQLGRG"
     gene            complement(22549..22821)
                     /locus_tag="PKN25_01790"
     CDS             complement(22549..22821)
                     /locus_tag="PKN25_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNV89656.1"
                     /translation="MARMVNCVKLGREAEGLDLPPVPGALGKRIFENVSKEAWQQWVK
                     YQTMLINENRLNLMDARARKYLAEQMEKHFFEGGADQVAGYVPPQA"
     gene            complement(22825..24243)
                     /locus_tag="PKN25_01795"
     CDS             complement(22825..24243)
                     /locus_tag="PKN25_01795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV89657.1"
                     /translation="MSACPAPGKAEDAELLAEIALGVRSVEAIFLPGLALRWISPSIE
                     ALTGFTAADCVAAADPFELLVFEDDRVFCRRMAQRVAAEESAQAFELRLRARDGRAVW
                     VQTHWRPRADGGLRLSAEHIQARKETEFTLLETVAELRRQEALRELYLVRSNDERQRL
                     AALLNLIRLGILFIDHDRRVLYHNRAMLAIWGYPPDTVLVGCRDAVLKERAPVVLADP
                     AGYLAHIHRTVRAKQSISEEYEFRFADGRIATDRSAVVAGGEAGRRIGRLWIYEDVTV
                     ARRTSMQLVELAERDELTGLYNRRRFHEELGRMLADAGRRGSRLGLLAFDLDGFKPIN
                     DSFGHQAGDEVLVRLARELGRTVRRNETLFRIGGDEFALLVPEGSTEGLCELAHRLVE
                     TVGAMRFVFEGRAARVTASVGIARFPDNAREGEALVAAADEALYRAKSEGRNRAAVSG
                     RRADESARMPSSNPDEPASRED"
     gene            complement(24240..25556)
                     /gene="argA"
                     /locus_tag="PKN25_01800"
     CDS             complement(24240..25556)
                     /gene="argA"
                     /locus_tag="PKN25_01800"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNV89658.1"
                     /translation="MDDATAQFVAWVRGAAPYIHAFRGRTFVVGFGGEVAAGKRAQSL
                     AYDCNLLAALGIRLVLVHGARPQIDAELARRGLEPRFHHGLRVTDADALECVKSAMAV
                     TRFEVEALLSQGLPNTPMAGSYIRVTGGNFITARPVGVVDGVDYQYTGAVRKIIAEEI
                     NADLDQQNVVLITPLGVSPAGEIFNLAMEEVAEAVAVALKAEKLIYLCDAPGLLGEDG
                     QLVESVTADEAQGKLETGEGLTEDLHLFLPCAIRAVKKGVARCHLIDHDLDGGLLLEF
                     FTHAGVGTVVSRDPLFRLREATVEDVAALVALISPMEADGTLVRRGRELLEQEIERFT
                     VVEHDGVLVGCAALYPFSEDNAAELACLAVTPEYRRAGLGDQLLKRIERRARVQRLER
                     LFVLTTRTAHWFRERGFSEIGPEALPRQKRELYNFQRRSKVFVKPL"
     gene            complement(25728..26327)
                     /locus_tag="PKN25_01805"
     CDS             complement(25728..26327)
                     /locus_tag="PKN25_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV89659.1"
                     /translation="MDAMRILMDEHQSLAAIIHAIRHMIGEIDAGRLRPDHKLLEAMV
                     HYLDAYPEKRHHPKEDAFLFGPLRARTHDADAALDRLEAEHAQADARIAVLEAAVKGY
                     AQDPVGGFEAFKTAFNDYAAFYRNHMMTEEREVLPQIRKHFTAEDWARANAGFIADDP
                     LGGTRASARAGEEDFAQIFSRLVAAAPAPIGLGAGPYKG"
     gene            complement(26367..27161)
                     /locus_tag="PKN25_01810"
     CDS             complement(26367..27161)
                     /locus_tag="PKN25_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV89660.1"
                     /translation="MNKQTQGSAAPSNADAKNPVLEVKDLCVSYGKVEALTDASLTVG
                     EGQIVTVIGPNGAGKTTMLSAIMGVLGSRGQVAFDGSVEGVPEVERMVARGMTLVPEK
                     RELFGEMSVEDNLVLGAFQRYRMGKRDHAQTLEEVYALFPRLKERRSQAAGTMSGGER
                     QMLAVGRALMAKPKLLMLDEPSLGLAPLIVREIFRIISELRRRGVSILLVEQNARAAL
                     QVADYAYVLETGQIAMQGPAHELADDPRVIEAYLGLGGKHQAMLST"
     gene            complement(27176..28969)
                     /locus_tag="PKN25_01815"
     CDS             complement(27176..28969)
                     /locus_tag="PKN25_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HNV89661.1"
                     /translation="MRTDRLVLGAFLALLLLVPAILSPYYVTLLNYIGLYAMVALGLV
                     LLTGVGGLTSFGQAAFVGLGAYTTAALTTATDLPGWLAWAGTSPWLTLAVGLLLTALV
                     AVLIGSLTLKLSGHFLPLGTIAWGISLYFLFGTMESLGGHTGISGIPAIGLLGWTLDE
                     GGEIYYLIWAFLLVAMLTTHNLLDSREGRAIRALKGGRVMAEAMGVDTSRSRMVIFVI
                     AALLACASGWLYAHMQRFVNPTPFGVHIGIEYLFMAVVGGAGYVWGAIVGAGVITVLK
                     QWLQNWLPSLLGATGNFETIVFGLLMVLVLHRAREGLWPILKKLVPVRALRKTIDPDA
                     EPLPRRTLPKAGELILEAKDVTRRFGGLIANNNMSLEVRAGEILALIGPNGAGKSTMF
                     NQISGVDTPTSGEVRFLGKPVAGHDSREIARMGMSRTFQHVKLLPTMSVLENVAIGAH
                     LRSDKGVLSSAWRLDRAEEARILKEAARQIERVGLAEYMYEEAGSLALGQQRILEIAR
                     ALCADPCLLLLDEPAAGLRFREKEALGELLRKLRAEGMATLIVEHDMDFVMSLVDRVV
                     VMEFGQKIAEGLPDEIQQNPAVLEAYLGGVE"
     gene            complement(28966..30003)
                     /locus_tag="PKN25_01820"
     CDS             complement(28966..30003)
                     /locus_tag="PKN25_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV89662.1"
                     /translation="MDMSIALILSQDGITNGAIYALLALALVLVFAVTRVIFIQQGEF
                     VAYGALTLAMMQSGVLPATVWMLVAMGALVTVLDGGLALKAGQGRRAASVAGWNLGYP
                     LALAAVVGGLAVNELPLAVQVLLTLAIVVPMGPMMYRLMYQPIASAPVLVLLIVSVAL
                     HIAMVGLGLLFFGAEGQRTPAFSEFSLELGPLMVSAQTLWVVVVSVLLIVALYQFFER
                     TLYGKALRATAINRVGAQLMGISPTLAGKLTFFLAAFIGALSGVLIAPITTIYYDTGF
                     LIGLKGFVAAIIGGLASYPLAAVGALAVGLLEAFASFWASAYKEVIVFTLIIPVLLWR
                     SLRSHHVEEEE"
     gene            complement(30118..31266)
                     /locus_tag="PKN25_01825"
     CDS             complement(30118..31266)
                     /locus_tag="PKN25_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV89663.1"
                     /translation="MKLRNTLLAALGLAFAATAAHAEIKVGVVLSATGPAASLGIPEK
                     NTIALLPTTIGGEKVSYIVLDDASDTTTAVKNARKLTVEDGVDVIIGSTTSPASLAMV
                     DVAAETKTPMISMAASARIVAPMDDKKRWVFKTPQNDQQMASAIVEHMVANKVTKVSF
                     IGFANAYGEGWYEQFKKLAEAKGIEIAASERFNPADTSVTGQALKLMSVKPDAVFIAG
                     SGTPSALPQKTLRERGYKGPIYQTHGVANNDFLRICGKDCEGTLLPVGPVQMARSLPD
                     SHPVKASALAYVEKYEAANGAGSVSSFGAYAWDAGVLLQAAVPAALKAAKPGSAEFRT
                     ALRDALEGVKEVAGATGIYTMSPDDHLGLDDRSRVMIEIRNGTWSLLK"
     gene            complement(31465..32280)
                     /locus_tag="PKN25_01830"
     CDS             complement(31465..32280)
                     /locus_tag="PKN25_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNV89664.1"
                     /translation="MSDELDGKHGVQSLEVGMSILRAMVTGKRSMMLKDIAQAADMPP
                     SKAHRYLVSLIRSGLVEQDRLTSRYDLGPFALNLGLVALDRVDRIRLGLSAIGDLRDL
                     TNETVALAVWSDRGPVVVRWERPRRPITVNVLTGTSLSLLSSAAGRVFAAWLPEQQTE
                     ALLRAELESGRVPAGIATLDDARRLLAEVRARGVAVISSGYFARGVEAAAAPVFNFKN
                     EINMSIALVGVEGTLDLLPHSPVLQALKEACDALSRRLGATLLPGVESGDHAT"
     gene            32364..32981
                     /gene="paaY"
                     /locus_tag="PKN25_01835"
     CDS             32364..32981
                     /gene="paaY"
                     /locus_tag="PKN25_01835"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HNV89665.1"
                     /translation="MNQLKVYAIDGIVPVVDPTAYVHPSAVLIGDVIVGPDCYVGPCA
                     SLRGDFGRLILERGSNLQDTCVMHGFPGTDTVVEEDGHIGHGAVLHGCRVGRNALVGM
                     NAVIMDNAVIGESSIVAAAAFVKAGVEMPPRMLIAGMPAKAIRALSEEEMRWKSSGTA
                     TYQDLTRRSLATMEETVPLAAVEPGRKRIHMPDVVPLVELRRREG"
     gene            complement(33080..34174)
                     /gene="paaX"
                     /locus_tag="PKN25_01840"
     CDS             complement(33080..34174)
                     /gene="paaX"
                     /locus_tag="PKN25_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HNV89666.1"
                     /translation="MPAESAWRSSSSLAYASAAASKARSGARRICPESIDFWPPTAAR
                     TSSSVTTSIQDRLEAFRKQDRVQAGSLIISVFGDAVLPRGGRIWLGSLIRLLEPLGLN
                     ERLVRTSVFRLARDEWLVSEPCGRRTDYMLTPSGLQRIEEASRRIYASAAQGWDRRWR
                     LILAVGELAAKDRERLRRALFWQGFGALDGDCFVHPSADLITAFDALVGEGLGGLLPR
                     LKPLMAADAALGAAASDADLVHAAWNLERLAQTYREFVDCYQPVLAQLRDGASESGDE
                     NAFLMRMLLIHDYRRLLLRDPGLPDVLLASDWPGQRARLLCRELYRRLLVPSERHIDA
                     HLRLADGRTPEASPRLLARFQDADPLRGAA"
     gene            34504..36171
                     /gene="paaN"
                     /locus_tag="PKN25_01845"
     CDS             34504..36171
                     /gene="paaN"
                     /locus_tag="PKN25_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HNV89667.1"
                     /translation="MPHPLLEKHRATLESALDAIATRGYWSAFPEMPSPKLYGEAAPD
                     EGKRAFEAHLGKPFELGQPGQTGWHGGESSPYGVALDVRYPVCDPETLIAAGLEAMKG
                     WQAVGADGRTGICLEILQRLNKQSFEIAHAVMMTTGQGWMMAFQAGGPHAQDRGLEAV
                     TYAWREQSFVPAETTWEKPQGKNPALVMKKHFQIVGRGVGLVIGCGTFPTWNTYPGLF
                     AALATGNAVIVKAHSNAILPAAITVRTIRTVLAENGIDPNLVSLCVATQRSVTQALAT
                     HPAVQSVDFTGSNVFGQWLIDNCRQAQVYAELAGVNNIVIDSTDSYKAMLGNLAFTLS
                     LYSGQMCTTSQAIFVPAGGIDTEDGHKSYDEVCADLARAVERFLSKPEVAHAVLGAIQ
                     SADTAERIDIANSGALGKVVLASQKLDNPEFPGAKVRTPVLLACDAADEKAYMEERFG
                     PISFIVKVADTAAGIALSERVVRTHGALTVGLYSTRQDVIDAMTEATWRGKVALSINL
                     TGGVFVNQSAAFSDYHGTGGNPSANASYSDSAFVANRFRVVQRRYHV"
     gene            36182..36976
                     /gene="paaG"
                     /locus_tag="PKN25_01850"
     CDS             36182..36976
                     /gene="paaG"
                     /locus_tag="PKN25_01850"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HNV89668.1"
                     /translation="MEYNNIQFAVEAGVARLTLNRPDKLNSFTGEMHAELRDALDRVH
                     ADKAARVLVLTGAGRAFCAGQDLADPDMASVGGKMPDIGNVVEKNYKPLVLRLQNLRV
                     PTIAAVNGIAAGAGASVALACDLVVAAKSASFLQAFSKIGLVPDTGGTWFLPQRVGMA
                     RAMGLAMLADKLPAEKAADWGLIWAAYDDAEFAAKVDALAAQLAAMPTKALVRTRQAM
                     HAAAGHTLEQQLSFEGGFMRELGWSPDYAEGVAAFMEKRAPKFTGE"
     gene            36973..38532
                     /gene="paaC"
                     /locus_tag="PKN25_01855"
     CDS             36973..38532
                     /gene="paaC"
                     /locus_tag="PKN25_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HNV89669.1"
                     /translation="MSVTDTQISKSAAQPLPASAVVAVVGTGAMGAGIAQVAAAAGHV
                     VKLLDNRPGAAAKAIEGIRAQFGKLADKGKMSTEAAQAAAERLIAVDQLAELADAALV
                     VEAIVENLEAKQKLYGDLENIVGADCIFGTNTSSISVTSIGAALQRPERLAGLHFFNP
                     APLMKLVEIVSGLATARVVADTLFATAAAWGKTPVHARSTPGFIVNRVARPYYAEALR
                     ILNEGGGDCATLDAIMRDAGGFRMGPFELMDMIGHDVNFAVTRSVWNAFYNDARFTPS
                     LIQQELVDAGFFGRKSGRGFYDYREGAARPAPQVAVAQHAPDEIVLHGHSAAVHALAD
                     RLFERNIPHTWGEANDGRIAETGGAVIFVTDGRTATQRATEMGIANVVLIDLALDYAT
                     ASRIAVGAAINCFAPAVGAAISLLQAAGFEVCRLGDTPGLAVMRTVAMLANEAADAVN
                     QGVCDAAGADAAMKLGVNYPRGPLEWADRVGLPAICAVLANLARFYGEDRYRVSPLIQ
                     QRVFAGKKIHD"
     gene            38525..38992
                     /gene="paaI"
                     /locus_tag="PKN25_01860"
     CDS             38525..38992
                     /gene="paaI"
                     /locus_tag="PKN25_01860"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HNV89670.1"
                     /translation="MTDAQPIPTDPQALAEAVTAAMWSRDRTAQALGMRIEHVGPGRS
                     RVSMTVRGDMVNGHHICHGGLIFTLADTAFAYACNAYNRNTVASGCNIDFVATGREGD
                     TLQAEAVERTLAGRTGVYDVTVRDSAGNTIALFRGKSYRISGEVIAGLAAENA"
     gene            39044..40369
                     /gene="paaF"
                     /locus_tag="PKN25_01865"
     CDS             39044..40369
                     /gene="paaF"
                     /locus_tag="PKN25_01865"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNV89671.1"
                     /translation="MPVKSPRPGDLDPIETASRDEISALQLQRLQWSVRHTYDNVAPY
                     RKRCEEAGVHPDDLQSLADLAKFPFMTKTDLRDNYPFGLFAVPRDKVARLHASSGTTG
                     KSVVVGYTKRDLDNWADVVARSLRAAGVRAGDMVHNAYGYGMFTGGLGAHYGIERAGC
                     TAVPMSGGQTEKQVQQIMDFRPDAIMVTPSYSLVIAEEFERLNIKPEDISLKIGIFGA
                     EPWGEGMRSEIERKLGIDAIDIYGLTEVMGPGVASECIETKDGPVIWEDHFYPEIIDP
                     DTGEVLPDGEEGELVFTSLTKEAFPVIRYRTRDLTRLLPPTARSFRRIGKITGRSDDM
                     LIVRGVNVFPTQIEEQIMRDRRLSGNYQIVLTRDGHLDNVEIRVEVQRELSGKLSRTE
                     TENIARELQHHIKTIIGISTKVNVMEFEAIPRTLTGKARRVIDQRPKQL"
     gene            40380..41585
                     /gene="pcaF"
                     /locus_tag="PKN25_01870"
     CDS             40380..41585
                     /gene="pcaF"
                     /locus_tag="PKN25_01870"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HNV89672.1"
                     /translation="MTQAFICDAIRTPIGRYGGTLASVRADDLGAVPLKALMTRNPQV
                     DWTAVEDIIYGCANQAGEDNRNVARMSGLLAGLPIEVPGTTVNRLCGSGMDAIGLAAR
                     SIKSGETELMIAGGVESMSRAPFVMGKAESAFSRSAAIYDTTIGWRFINPLMKKLYET
                     HSMPQTADNVAADFDISRADQDAFALRSQQRWAAAHAAGRFKDELVPVVIPRKKGDPI
                     VFDTDEHPRPETTLEMLAKLKGVNGPELSVTAGNASGVNDGACALLLASDAAAAKHGL
                     TPRARVVAMATAGVAPRIMGFGPAPAVRKVLAKAGLTLDQMDVIELNEAFAAQGLAVL
                     RDLGLADDEERVNPNGGAIALGHPLGMSGARLVTTAAYELQRRNGRYALCTMCIGVGQ
                     GIAMIIERV"
     gene            41601..42386
                     /locus_tag="PKN25_01875"
     CDS             41601..42386
                     /locus_tag="PKN25_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNV89673.1"
                     /translation="MNYETLAIERRGAVATIWMDRPAVFNAFNEQLIAELAAACAELD
                     ADAGVRVVVLAGRGKHFSAGADLNWMRRASDSTEAENLADSRKFAAMLRTLSGMSKPT
                     IARVQGAALGGGTGLTAACDMAIAADDASFATSEVKFGIIPAVISPYVLRAIGPRHAL
                     RYFQSAERFGAAEAKAMGLVGEVVTLDELDAAVDRLVQALLACGPQAQLAAKQLIAAL
                     AGRPIDDAVAEETAGRIARQRATAEAREGFTAFFEKRSPAWMQ"
     gene            42503..43510
                     /gene="paaA"
                     /locus_tag="PKN25_01880"
     CDS             42503..43510
                     /gene="paaA"
                     /locus_tag="PKN25_01880"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HNV89674.1"
                     /translation="MYTQAMDIPDDSGKKLAAVSNATSEQEAAFEARIDADGRIEPQD
                     WMPEAYRKTLVRQISQHAHSEIVGMLPEGNWISRAPSLKRKAILIAKVQDEGGHGLYL
                     YSAAETLGTSRDQMLEGLHSGKAKYSSIFNYPTLNWADVGVIGWLVDGAAIMNQVPLC
                     RCSYGPYARAMVRVCKEESFHQRQGYEALLVMMKGTDEQRAMVQDAVNRYWWKCLAMF
                     GPPDADSPHSAQGMRWGIKRISNDDLRQKFIDATVPQAKVLGVTLPDPDLKWNEARGH
                     YDYGQIDWAEFWNTVEGNGPCNKERLATRVKAHNDGQWVRDAAQAYARKQKERALKEA
                     A"
     gene            43507..43803
                     /gene="paaB"
                     /locus_tag="PKN25_01885"
     CDS             43507..43803
                     /gene="paaB"
                     /locus_tag="PKN25_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HNV89675.1"
                     /translation="MNTPALKDWPLWEVFVRSKQGLEHKHCGSLHAADAAQALHMARD
                     VYTRRQEGVSIWVVPSVAITASNPDDKGELFDPAADKIYRHPTFYEIPEEVGHM"
     gene            43803..44585
                     /gene="paaC"
                     /locus_tag="PKN25_01890"
     CDS             43803..44585
                     /gene="paaC"
                     /locus_tag="PKN25_01890"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HNV89676.1"
                     /translation="MSTPIDYLLHLADNAVVLGQRNAEWCGHGPILEEDMALANVSLD
                     LIGQARLLYQRAAELKGGDATEDKLAYFRGPHEFRNYTLLELPHHGALVGYAAADRDY
                     ATTIVRNFLYSALMVHVWDGLQQSADASLAAIAAKSLKETSYHLHHSRDWLVRMGDGT
                     EESHARAQAAVNHLMPYTEEFWTTSPAEGAAVAAKIGIDVWALRPAWDALVNEALAEA
                     TLTRPNFHGYVTEGKIGLHSEHLSYLLGEMQGLARAHPGATW"
     gene            44597..45109
                     /gene="paaJ"
                     /locus_tag="PKN25_01895"
     CDS             44597..45109
                     /gene="paaJ"
                     /locus_tag="PKN25_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HNV89677.1"
                     /translation="MSSVVDKVWATLEPLTDPEIPVVTLRELGILRDVRETAEGIEVV
                     ITPTYSGCPAMGQIEDDVRASLEQAGIAARVITQLAPAWTTDWMSDEAKEKLRAYGIA
                     PPHRTCGSSGGAEVSVLKFVRRQPTAAVACPQCGSTHTVVTSEFGSTACKALYKCLDC
                     QEPFDYFKPY"
     gene            45129..46226
                     /locus_tag="PKN25_01900"
     CDS             45129..46226
                     /locus_tag="PKN25_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNV89678.1"
                     /translation="MSAPRFQELAIKRVSPEAAGAVAITFAIPEAERERFAFEPGQFL
                     TLRATIDGQDVRRNYSISSPRSRLAREGELEIGIRPVEGGLFSNWATRAIKAGDTLQV
                     MPPDGRFVVKKKRAIHRVGFAAGSGITPILSIAASTLEEQPDSKFTLIYGNRRMSTVM
                     FNEDLQDLKDRYRDRLTMIHVLSRQAQEVDLLQGRIDGPKVRAIIDALLPVGSMDEVF
                     ICGPDEMITATETALVEAGVPADRIRTERFTANLPAGAHPVGASSTAEAVAAATKDIT
                     MVLVLDGKEHEIAIGPDEHLLDAGLNAGLDLPFSCKAGVCCTCRAKVTEGEVVMDKNF
                     TLEADEVAQGYVLSCQARATTKRLKISFDER"
     gene            46400..49060
                     /locus_tag="PKN25_01905"
     CDS             46400..49060
                     /locus_tag="PKN25_01905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV89679.1"
                     /translation="MAGSHALSTAFEPSSIAVIGASDAPGSVGRVIFRNLVEGGYRGT
                     LYAVNPKYGSIQDHPCHRSVEDIGRPVDLAIIATPARMLHSVTAQCGRSGVRNAVIIS
                     ASGNALERRIVETARETRLRLIGPGSLGIARPGSGINAALTRIAVKPGEIALISQSGA
                     MCSVVLDWALTNDVGFSSVISLGGTVDIDYGETLEYLIHDTQTRYILLHVDHIRNARQ
                     FMSALRSAARVKPIILLKAGRHGRSDNNAADADNAGLADVVFDAAMRRAGVVRVQNID
                     QLFFAAKALSCGFRPREDSLAIVSNGSGPADLAADRAKDLGTGLVGLGSPTVATLERL
                     GGRERHSHNPLDLGGDASAQLYRDAILALSEDAAVSNVLVILSPHALVDPVEVAQDII
                     DLAAQVRLQLCCCWMGGGQVAEARALLARAGLPVFSSPEAAIELFHNISRFHHNQTLL
                     LQTAGGGSAAAYRRTGGARVLIETLLNQRRHILSAMESKALLRSFGIPVTHTTVARDA
                     TEALFVAEQAGFPVAMKVDAPELASKSESGGVRLNLNTAESVWSAFHDIVDAVRLRHP
                     EVQINGVSIEPYLYRPQARAFRIQVHRDAIFGPVISLGAQDASAPRTFALPPLNPVLA
                     RDLIDSAIPAAAQGPRSQGGALNRAAIEQVLVSVSDLVCELPWVRELAIDPLFIDGDG
                     AIAADARVVIDQGLPAGSDRYAHMAIHPYPLHLVQDWKVGDGRAVRTRPVRPEDANLV
                     LGFFDGLSAETRFMRFMEELQELSPALVARFTQIDYDREMALIATTIDDAGAEHMVGS
                     ARYSLAADGESVEFALVVADDWQRHGLGRRLMGALIEVARGKGYRNIFGDVLGKNAKM
                     LRLMHGLGFLVQPHPEESALRRVVKVLHGK"
     gene            complement(49088..49810)
                     /locus_tag="PKN25_01910"
     CDS             complement(49088..49810)
                     /locus_tag="PKN25_01910"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNV89680.1"
                     /translation="MISRFHFPDRLPAGGEVALPEALAHHALRVLRLRDGEAVVLFDG
                     SGGEVDARLAVRGKAVFARLGEARAVERESPLRIVLVQALASGDKMDWVVQKAVELGV
                     HAIQPVQAERSVLRLAAERADKRVAHWQQVAIAACEQCGRNRVPEIRPLQPLAHWLGA
                     HRAAPGYVLAPGGAKGFADEPEPKDEVHLLVGPEGGWSEGEMAAFDAAGCRRVRLGPR
                     VLRTETAGLAAVAALQARWGDY"
     gene            49881..50690
                     /locus_tag="PKN25_01915"
     CDS             49881..50690
                     /locus_tag="PKN25_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HNV89681.1"
                     /translation="MPTAAHRLARARALESMVRDIARAEILPRYLKSARNRKADGSLF
                     TEADMASQQRFSEALPAFLPGAVLGEEMTAAEQAALWKEGARGLWCIDPIDGTTNFAN
                     GIPLFAVSIAYLIDHEPVIGVVYNPITDESFYAARGAGAFLNGTELPLRAGATELRDA
                     VAGVDFKRISHHLGDELAVRPPYYSQRNFGSSALEWCFVAAGRLDVYLHGGQMLWDYA
                     AGRLILAEAGGIAEALDGGVLMSGPAIKRGVIAATSPALFAEWSAWVKARS"
     gene            50761..51636
                     /locus_tag="PKN25_01920"
     CDS             50761..51636
                     /locus_tag="PKN25_01920"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HNV89682.1"
                     /translation="MSMKHPIIAVTGSSGAGTTTVKHTFEAIFHRENVNAAIVEGDSF
                     HRHTRAEMKTLIEEAERAGLRGISHFGPEANLLSELESLFRAYGEAATGRRRYYIHNE
                     AEAMRWNLPLGTFTEWEDLPVGTDCLFYEGLHGALVTDEADIASHVDLLIGVVPIINL
                     EWIQKLHRDTKLRGYTAEAVQDTILRRMHDYVHYIVPQFARTHINFQRVPVVDTSNPF
                     IARDVPTQDESMVVIRFKDPRGVDFPYLLRMIHDAFMSRANTIVIPGGKLDLAMQLIL
                     TPLIWKLMERRRQWV"
BASE COUNT         8114 a        18830 c        17216 g         7511 t
ORIGIN      
        1 ctcaagaagc tcgacaaggt ggcctacatc cgcttcgcgt cggtgtatcg caacttcgcc
       61 gacgtcgacg agttcgccga ggtgatccgc gaggtgcaga cgcgcccgcg gcgcggcaag
      121 gccgacaagg gcggagcgga ccccggacat gaccttttcg gcggctgacc acgccgccat
      181 ggcgcgcgcg ctgcagctcg ccgcccgcgg cctcgacacc acctcgccca acccgcgcgt
      241 gggctgtgtg atcgtgcgcg acggcacgac cgtcggcgag ggctggcacg cgcgcgccgg
      301 cgaggcccac gccgaagtcc acgcgctgcg ccaggccgga gcggccgcgc gcggtgccac
      361 cgcctacgtc accctggagc cgtgctcgca cttcggccgc accccaccct gcgccgacgc
      421 gctgatcgag gccggcgtgg cgcgcgtgat cgccgccatg gaagacccca acccgctcgt
      481 cgccgggcgc ggtctcgccc gcctgcgcgc ttccggcatc gccaccgcgc acggcctgct
      541 cgccgaggcg gcgcaagagc tcaacatcgg cttcgtttca cgcatgacgc gcggtcggcc
      601 ctgggtgcgg ctcaaggtcg ccggcacgct cgacggcaag agcgcgctcg acaacggcgt
      661 cagccagtgg atcaccggcc cggccgcacg ccgcgacggc caccgctggc gcgcgcgcgc
      721 atgcgcgatc ctgaccggca tcggcacggt gcgcgaggac gatccgcaac tcggcgtgcg
      781 cgacgtgccc tgcgaacgcc agccgctgcg catcctggtc gatgcgcgcc tggaggtgtc
      841 gccgcaggcc cgcatcctgc agggcgaacc catcctgatc gccaccggca gcacggatca
      901 agcccgcatc gacatgctgc gcgcggccgg ccacgagatc gtcttcgtgc ccaacgcggc
      961 cggcaaggtg gatctgggtg cgctgctgcg cctgctgggc gagcgcggca tcaacgagct
     1021 gcacgtcgag gccggcttca agctcaacgg ctcgctgctg cgcgagggct gcgtggacga
     1081 gctgctgatc tacctggcgc cgatgctggt cggcgacgcc gcgcaggggc tcttcaacct
     1141 gcccgcgctc acccgcctgg aaggcgccac aaggctggac atccgcgacc tgcgccgcat
     1201 cggccccgac ctgcgcctga tcgcgcgacc gctagcgcag ggctgattcc accgctccgg
     1261 cgcatcgcgc cgtcccgcac accgcgcagc cggggtcgcg cccgagcgcg acactgcgcc
     1321 attccatcga caagccgtcg agcagcagca agcggccgtc gagcgtgcgc ccgcaaccaa
     1381 tgaggacctt gagcgcctcg gcagcctgga tggtgccgac gatgccgacg atgggggcga
     1441 acacgcccat caccgcacag cgcacctcct cgatgtcctc gccttccggg aacaggcagt
     1501 ggtagcagcc gctgtcgggc ttgcgcaggt caaacaccga gacctgaccg tcgaagcgga
     1561 tcgccgcgcc cgacaccagc ggcttgcggt gcttcacgca ggcgcgattg atggcgtggc
     1621 gggtggcgaa gttgtcggtg cagtcgagca ccacgtcggc gcgccccacc tcggcctcga
     1681 gtgcctcgcc ctccaggcgc gcctggatcg gctcgagccg ggtctgcggg ttgagccggt
     1741 gcagcgtggc acgtgccgag gcgaccttgg gcaggccgac catgtcggcc gagtgcatga
     1801 tctggcgctg caggttggtg agatcgaccg tatcgtggtc ggcgagcacg agggtgccga
     1861 ccccggcggc ggcaaggtac atcgccgcgg gcgagcccag cccgcccgcg ccgaccacca
     1921 gcgcgcgcgc gtcgagcagg gcctgctggc cttcgacgtc gatctcgggc agcaggatgt
     1981 ggcggctgta gcgcagcagc tggtcgtcgt tcatcgcccg attacttgta ttcgcgcagt
     2041 tcgagcgggg tgtcgtcgtg gtcgccatgc acgtggtggt cccaggcctg gatgtagacc
     2101 tcgttggact gcttgatcag gcgttcgggc gacagcaggt tgtggcgctg ggtctcctcg
     2161 ctgaaataca cgagtgcccg caccagcttg atgtagagcg aattctccag cgcaaagccg
     2221 gcctcgcgca gcgcgtcctc gagcgactcg agcgaatagt cgagcaccga gtagcgcacc
     2281 acgatgccca gcggatcgtc ggtgcgctcg agctgcaggc ccttgagcag ttgcagcgac
     2341 ttccaggcgc gctccacctg cccgggcggg agcggcttga agcggatctc gcgatgcttg
     2401 tggatatcgg cgcccggcaa gggccggtcg tgatgccggc ccgccagctt gtaggcagtt
     2461 tccctgcgca tgatcgtccc cccggccgga ctcccggctt actgtttcgc cgccgcaggc
     2521 tgcgcggccc cggccgccgc ggcctgtccc ccggccgctt cggcctcgac ggccggcgcg
     2581 gcgtgggcga tgtcctcgcc cttgagcagg cggatcgcct gcttgagctg gtggtcgttg
     2641 tcgcccgcga gctcgaagcg cgcagtctcg gccgcggttt cttctgccgg cgcgccgtcc
     2701 gccttgccgg cagcggcctt cgcggcctcc acggcgccct tgtcgttctc gagatgcccc
     2761 agcagatcgg cctcgcgcac gcggcggccg gacccgttgg cggtctcctc gacgacgatg
     2821 tcgggcgcga tgcccttggc ctggatcgag cgcccgctcg gcgtgtagta gcgtgcggtg
     2881 gtgagcttga tcgcggtgtt gttgttgagc ggcaggatgg tctgcaccga gcccttgccg
     2941 aaggtctgcg tgccgatcac cctggcgcgc ttgtgatcct gcagcgcgcc ggcgacgatc
     3001 tccgaggccg aggccgagcc cgcgttcacc agcaccacca tcggcacgtc gcgcgcatag
     3061 gccggcaggc ggcgcaggaa gtcgtcgcgg gtgccgcgca ggtagtcggc ctggatggcg
     3121 cggtactcgc gcttggcgtc ctcggtgcgg ccgtcggtgc tcaccacgag ggtctcttcg
     3181 ggcaggaagg ccgccgacac gcccacggcg ccgtgcagca ggccgccagg gtcgttgcgc
     3241 aggtcgagta ccaggccctg caccgggccc tgagcgccga gcttggccag gtgctcgacc
     3301 accgacgcgg cggtgttctc ctggaactgc gcgatgcgca ggtaggcgta gccgggctcc
     3361 accaccttgg acttgacgct ctgcaccttg atgacttcgc gcgtcagctt gatcacgatc
     3421 ggctgcgact gccccttgcg catgatcgtg aggatgatgt cggtgcgcgg cttgccgcgc
     3481 atgcgcttga ccgcgtcgtt gaggctcatg cccttgaccg gggtgtcgtc cagcttgacg
     3541 atcaggtcgc ccgccagcac accggcgcgg aaggccggag tatcctcgat cggcgagatc
     3601 accttgacga agccgtcttc catgccgacc tcgatgccga gaccgccgaa ttcgccctgg
     3661 gtgccgacct ggaggtcctt gaaggcctcg gcgtcgagat aggcggagtg cggatccagc
     3721 ccggagagca tgccgctgat cgagtcggtg atcagcttct tgtcctccac cggctcgacg
     3781 tagccttgct tgatcgcgtt gaagacgtcg gcataggcgc gcaactcgtc gaccggcagc
     3841 ggcaggggcg cgctgcggtc ggcgttggcg gagaaattga ggctgatcat gatcccggcc
     3901 accatgccgg tcatgacgag gccgaactgt ttcagcttgc tgctgcgcat gagaactcca
     3961 gtagtgcgcc gaccgccccg gtcggccgtc ccgtctagtt gagccgcatc cattgcatcg
     4021 gatcgagcgg ctgcccctgg tgacggatct cgaagtataa acccgaatcg ttgcccaccc
     4081 cggcggcgcc cacgctggcg atcggctcgc caccgccgac gcgatcgccc acttccttga
     4141 gcagggaatc gttgttgccg tagatggtca ggtagtcgct gccgtggtcg acgatgatga
     4201 ggttgccgta cccgcgcaac cagtccgaga agaccacttc cccgccggcc accgaacgca
     4261 cttcggcacc gttgccggcg cggatgaaca cgcccttcca ggtggttcca ccctccgccc
     4321 ttggagcacc aaagcggccc acgagttctc cgcgcaccgg aaagcgcagc tgcccgcgca
     4381 gctggttgaa gcgcacgccg gtgggcgtcg cactggcggc gtcgcggacc tcgccgacca
     4441 ccggctcggg gcgctgcgca cgctccaccc gtgccggcgg cggcgcctcg aggcgtggca
     4501 ccggcgccgg ctccgcgcgc gctgcggtct cgccacgctg ttcgtgctcg gcgcgnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccggc gccggctccg cgcgcgctgc
     4681 ggtctcgcca cgctgttcgt gctcggcgcg tgcccgcgcc gcacggcgct ccgcctcgcg
     4741 ctgcgcggct tcgcgacgtt cggcctcgcg ctgcgcggcg gcgcggcgtt cggcctcgcg
     4801 ctgctccgct tcacggcgtg cggcctcgcg cgcggcggcg aggcgggcgc ggcgagcgag
     4861 ggtctcgatg agcttgccga gacgctgttc gtcctgctgc agcgcatcga cctcgctctt
     4921 cttcgcctgc agctcgcggc tcacctcggc gagcgcggcc ttgcgccgcg actgctcggt
     4981 ctgcagctgc tgctggcggt cgcgctgctc gctctcgagc gtggcgagac gatcgcggcg
     5041 ggcgctgatc tcgtcggcgc ggcgccgggt ctcggagagg tcggtgcgca ggccttcgat
     5101 gagctccagg cgggcgcgac caaggtgctc caggtagtgc gcgtcgcgcg cgagctggtt
     5161 ggggtcgcgt gccgacagca gcggcgccac accgtcggcc gcgccatgca cgtagtggcg
     5221 acgcacccag tcggcgagct caccgcggcg ggcgtcgatg cgcgcggcgg tctcgcgctg
     5281 ctcgccttcg agcaccgcga gccgcttctc ggcgttcctg cgctcgccct cgatgcgggc
     5341 gagctcgcgt acggcgcgcg acaccgaacg ctcggagtcg accagcgcct tctgcgcgct
     5401 ggtgcgatcg gcctccgcct tcgccatctc ctcctgcaac tcgcggatac gctgcttgag
     5461 ctcgtcgagg tcggagcgcc gctcggcgat ctcgccgccg gcctgggcga aggccgggcc
     5521 gggcacgaca aggagggcgg cgagcgcggc gatgcacgcg cctgcgatcg gcgacttcag
     5581 gcttctgggc gacaacgggg tgggatccgc ggagagttgc gaggggcgag caatgctgga
     5641 cgggggcgag gacggagggc agtcggacaa gggacccgat gcggtgaagg gtgcgcgagg
     5701 cggcggctcg tgaccggcac gcggccgctc ggacgcgaga tctctcatgc atcgggacaa
     5761 cgcgttattt tatactagcg ctttggttct gccggcccca tcgtggacaa agacgcaatc
     5821 accaccctga tcgacaagca cgaacacatc gagaccgccg cccgcgcgct caaggcgatc
     5881 gcgcatcccc tgcgcctgaa gatcctctgt gtgctcggca gcggcgaaga atgcgtgcag
     5941 gacatcgtcg acgcggtggg aacctcgcag agcaacatct cccagcatct cgccatcctg
     6001 cgcgacaagg gtgtgctgca gacgcgcaag gacgccaacc gcgtctatta ccgcgtcggc
     6061 gacgagcgca ccctgcagct gatcgtgctg atgcgcgagg tcttctgcgc cgacgacgac
     6121 gccgaacgct gaccccccaa ccccgcaacc ggagcatccc cttcgtggaa ttcctgcaac
     6181 acaactggta ctgggcggcg ctcgccgccg cctcgggcac ctggctgatg gtcgaactcg
     6241 tgcgcagccg cggcgacgac acccagctct cgcccgtgga ggcgaccctc ctcgtcaacc
     6301 gcgaggacgc gatcgtcatc gacgtgcgcg agcagggcga gtacgcccag ggccacatcc
     6361 ccaacgcgcg ccacatcccc gccggcgaga tcgagcgccg cggcaaggag atggaaaagt
     6421 ggaaggacca cccggtcatc ctttgctgca ccaccggcgc gcgctcgaac agcgccgcag
     6481 gcgcgctgcg caaggccgga ttcaaccgca tctacaacct gcgcggcggc atgatggagt
     6541 ggcagaaggc ggggcagccc gtcagccgca agaggaagtg agcatgcagc ctccgatccg
     6601 catgtacgcg accgccacct gcccctactg catccgcgcc gagcaattgc tgctcgacaa
     6661 gggcgtggtc gggctggaca agatccgcgt cgatctcgac ccctcgcgtc gcgaggagat
     6721 gatgaagctg agcggacgcc gcaccgttcc gcagatcttc atcggcgact accatgtggg
     6781 cggctgcgac gatctcctcg ccctcgagcg cggcggcagg ctcgctgcgc tgctgcgcgg
     6841 cgagagcgcc tgagcgcggg ttcgtacccg cggcacgcga gacgcccccg cccccccatc
     6901 tccgacgctc cccgccgagc atcctttttc cccgcggccg caggcgcggc cgtacccacc
     6961 caaaccggaa cccgattcat gagcgaaaac acccaacccg tcttctcgat cgaaaaactc
     7021 tacgtcaagg acctctcggt cgaggtgccg ggtgccccgc agatcttcct cgagcgcgag
     7081 accccgcagg tgaacgtgca actgcgtacc gaaggccagg tggtcgagga aggcgtgtac
     7141 gaagtcaccc tgaccgtcac cgtcaacgcc cgcatcggcg aggaacgcaa cctcttcctg
     7201 gtcgaggtcg cgcaggccgg catcttccag atccgcaacc tccccgaggg tgagctggag
     7261 ccggtgatga tgatcggctg ccccaacatc ctcttcccct acgcccgcga ggcggtgtcg
     7321 gacgcggtca cccgtgccgg tttccagccg gtgctgcttt cgccggtcaa tttcgaatcg
     7381 ctctaccagg cgcaacgcca gcaggccatc gagcaggcga acggcgaagt tcccatccag
     7441 tgaggcgtcc cgcatgaagc gcgcgcgccg tccgtccccg cagatacctt cggcgccgct
     7501 cggccgcgcg ctcctgatgc tcgccgtgtc cgcccccctg ggcggcgcgg ccttgccggt
     7561 cgccgcgcag gcgatcgaat accgctcggt cgcggcttcg accctgctgc gcgagcagcc
     7621 ggcgccggat gccgaggcgc tgttccgcct gcgcccgggc acaccggtcg agatcgtcgt
     7681 gcgcgaggac ggctggatgc gcgtgcgcga ccctgccggc gggttcggct gggtcgaggg
     7741 cggcgccctc gtcacgcgcc ggacggtcat cgtcaccgcg gagcgcgcga tcgtgcgccg
     7801 cgccgcgcag gagaccgccg cgcccgcctt cgaagccacg cgcaacgtcg tcctcgaact
     7861 gctcgaaccc gccagcgagg gctgggcgcg cgtgcgccac gtcgagggct tcgaaggcta
     7921 cgtgcacgcc agcgaggtat ggggactgtg agccgccgcc gcatcgccgt gttcggcgcg
     7981 ggcgcctggg gcaccgcgct cgcccaagcc ttttccgcca gccacgaggt ggtcctgtgg
     8041 ggccgcgacc gcggccagct cgaagagatc gcccgcatcc acgagaacac ccgctacctg
     8101 cgcggtgtcc gcctgcgcga cacgctcgcg gtcgaggccg acttcgcgcg cgccgcagcg
     8161 caggccgagc tgcatctggt cgtcaccccc ctcgccggcc tgcgcaccac ggtgcgcgag
     8221 ctgcgtcggc tcgctcccgg gcgtgcgctg ctgtgggcct gcaagggctt cgaggccggc
     8281 accggcaagc tgccgcacga gatcgtcgcc gaggagtgcg gcgccgggtc cgcgagtggc
     8341 gtactcaccg gcccgagctt cgccgccgag gtcgcgcgcg gcctgcccgc ggcgatcgcg
     8401 ctggcggcac acgacgccga tttcgcccgc cactgggtgg gcgtgctgca ccagccccgc
     8461 ctgcgcatct acgccaacac cgatctggtg ggctgcgagg tcggcggcgc ggtcaagaac
     8521 gtcatggcga tcgccgccgg cgtctccgac ggcatgggct tcggactcaa tgcgcgcgcc
     8581 gccctgatca cccgcggctt ggccgagatc gcccgcctcg cgcgtgcgct cggcgcgcgc
     8641 ggcgagaccc tgatgggcct ggccggcatg ggcgacctga ttctcacctg caccggcgac
     8701 ctctcgcgca accgccgcgt cggcctggcg ctggccgagg gcaagggcct cgccgacatc
     8761 ctgcgccagc tcggccacgt tgccgagggc gtgtcgaccg cacgcgaggt ggtcggcctc
     8821 gcccgccgcc atggcgtcga gatgccgatc tgcgaagcca tcgacgctct cctccatcac
     8881 ggactcgacg cgcgcggcgc cgtggagcgc ctgctcgcac gcgacccgcg cgcggagtga
     8941 gcgcgcgcgg cgcggctcgg ctcagtcgcc ctgcgcgacg gcgccggcaa agtcgtgctg
     9001 gcgccagccc tcgtacacca ccaccgccac cgcgttcgac agattgacgc tgcggttggt
     9061 cgggcgcatc gggatgcgca cccgccgctc gggcgcgaac tcgcgcagga tctcctcggg
     9121 caggccgcgg ctctcgggac cgaagagcag cgcatcgcca ggctggaaat ccacacggtc
     9181 gtgacgccgg ctcgcacgcg tgctcatggc gaacatgcgc cgcccggcga gcacctcgcg
     9241 gcagtgcgcc cagtcgggat gcacggtcag gcaggtgaga tcgtggtagt ccaggccggc
     9301 gcgggcgagc tgcttgtccg acaggtcgaa gccgagcggc gccaccagat ggaggcggca
     9361 gccggtgttg gcggcgaggc ggatcacgtt gccggtgttg ggcgggatct cgggctggta
     9421 gaggacgatc tcgaacatcg ctggatagcc aaggttggcc gggtgcaggt gcatggccgg
     9481 gcggaaatcg gctgcggcaa gaccgctccg caagccgaag cgagtgcagc ggagccgccg
     9541 gccggcgagt ctagcaggcc gccaccaccg cgggctcaag agggcgaagg cgtgcgcgcc
     9601 acgaccaggt tgccgaccca gctcgcccct cgccccttga ggcagcgcgc aagctcgtcg
     9661 agcgtggcgc cggtggtcat cacgtcgtcg accacgatca ccctgcgccc gctcaggtcc
     9721 tccacgcact cgaaggcgcc gcgcgggctg cgcaggcggg cctcgcggtc gagcccggcc
     9781 tgcgccggca ggtcgcgcag cttgcgcacg acggcgagcc cgagcggcag gtcgcgcgca
     9841 cgcgcgaatc cgcgcgccag ctccaccgcc tggttgaagc cgcgctcggc gagcctgcgc
     9901 ggatgcagcg gcatcggcag cacgagatcg gcccccgcgc cgtcttccgc agcgaaatcg
     9961 gcgccgcatc gggccaacgc atcgccaagg taacggccca ccgcgagctg gtggcggtac
    10021 ttgagggcat ggatcatcgc gtcgaccggg aaggcgaaat cgaacaccgc ccgcgtcgca
    10081 tcgaaggccg gcgcgctgcg ccggcaggcg ccgcaacggc caccatccag gccgggcagg
    10141 ccgcattgcg ggcacgcggt ggaaggacgc cgcggcagct cgtcggcaca ggcggcacag
    10201 acggcctcgc cgccgctcgt cgcaccgcag acgaagcagt cctgcggcgc gagcacgtcc
    10261 atcgcatggt tgaacaggac gcggagggga tgcagtgaat ttgacaagat ttccaccgat
    10321 ggaagaacat gcgggcctcg taattcgtaa ttacagcctg gaaagcatta catgacaacg
    10381 acttccgcga cgatcgaacg ccccgcagcg cccccgtccc gccaccagtg ggcgaccgcc
    10441 gaggtcgagg cgctcttcga gctgcccctg ctcgagctcg tcttccgcgc ccagcaggtg
    10501 catcgcagcc acttcgaccc gcaggaagtg caacgctcca ccctgctctc gatcaagacc
    10561 ggcggctgct cggaagactg cggctactgc tcgcagtccg cccgccacga cagcggtctc
    10621 gcccgccagc gcctgctccc ggtcgacgag gtgctcgcca acgcgcgcgc ggccaaggcg
    10681 cgcggcgcca gccgcttctg catgggtgcc gcctggcgcg ggccgaagga ccaggacctc
    10741 gccccggtgc tcgagatggt gcgcgaggtg aaggcgctcg gcctgcagac ctgcgtgacc
    10801 ctgggcatgc tgaaggaagg ccaggccgag cagctcgccg acgccggcct cgactactac
    10861 aaccacaacc tcgacaccgc acccgacttc tacggccggg tgatcaccac ccacaccctg
    10921 caggaccgcc tcgacaccct cgacaaggtg cgcggcgccg gcatcaacgt atgcagcggc
    10981 ggcatcgtcg gcatgggcga gaaccgccgc cagcgcgccg cgctgatcgc ccagctcgcc
    11041 aacctgccgg cgccgcccga gtcggtgccg atcaacaacc tggtccaggt cccgggcacg
    11101 ccgctcgcgg gcgccggggc gctcgacccc ttcgaattcg tgcgcacgat cgccgccgcg
    11161 cgcatcacca tgccgagcag ctacgtgcgc ctgtccgccg gccgcggcga gatgagcgac
    11221 gagctgcagg cgctgtgctt cctggcgggc gccaactcga tgttctacgg cgagcgcctg
    11281 ctcaccaccg acaaccccga cgccgaccgc gacgaccgcc tgttcgcgcg cctcggcctg
    11341 cgcgcaattt gagcggcgag cgcatggaca cccccacccc cggccaggac gtcagcgccc
    11401 gggacgcccc ccccgggcag gcccccgccg cgccgggctt cgccctcgac cgcagcctgc
    11461 tgcgccgccg cttcgagcgc gccgcgccgc actgcgacgg cgccgacgcg ctggcgcgcg
    11521 aggtcgcgcg gcgcatggac gagcgcctcg actacatccg cctgagtccg cagcgcgtgc
    11581 tcgacctcgg ctgtggcacc ggggcagacc tgccgcgcct ggccgaacgc ttccccgcgg
    11641 ccaccctgct cgctgccgac ttcgcgcccg cgatgctcgc ccgcgcgcgc gcgcgcctgc
    11701 gcccgcaggg cggcgggctg ctgcgccgcc tgatcggcgg gcgccccgcc gcggcgccgc
    11761 atttcgtcgc cgacgcgtgt gcgctgccgc tggcgcacgc cagcctcggg ctggtgtggt
    11821 ccaacctgat gctgccggcg ctcgacgacc cgctgcccgc cttccgcgag gtgcaccgca
    11881 cgctgcaggt ggacgggctc ttcatgttct cgacgctcgg ccccgacacc ctgcgcgagc
    11941 tgcgcgccgc gctgcccgcc acggcgggtg agcgggtgca ccgcttcatc gacatgcacg
    12001 acctcggcga cgcgctggtg caggccggct tcagcgaccc ggtcatggac atggagatgc
    12061 tgaccctgac ctacgccgac ctcgacggcc tcttcgccga cctgcgcgcg agcggtggca
    12121 acaacgccgc cagcactcgc ccgcgcgggc tctccggccg gaccggctgg gaggctgcgc
    12181 gcgccgccta cgagcgcctg cgccacgacg gccgcctgcc ggcgagcttc gagatcatcc
    12241 agggccacgc ctggaagccc gccccgaaga ccaccgcgga cggacgcgcc atcatgcgct
    12301 tccagccccg cccgcccgcg cgctgagccc ctcctccccg cacgatcctg ccccgagcga
    12361 agaagatgtc ctcccgtccc gtctggctgt tcgacctcga caacaccctg cacaacgcca
    12421 gtccgcacat cttcccgcac atcaaccgca gcatgacgcg ctacctcgag cagcacctcg
    12481 ggctcgccct cgacgaggcc aacgcgctgc gcatgcagta ctggcaccgc tacggcgcca
    12541 ccatgctcgg cctgatgcgc caccacggca ccgaccccga ccacttcctc gtcgagaccc
    12601 accgcttcga ccgcctgcac gagatgatgg tgttcgagcg cgcgctgcgc ggcatgctgc
    12661 gcgccctgcc ggggcggcgc atcgtgttct ccaacgcacc gcgccattac gccgaggctg
    12721 tgctggagat catgggcgtg cgccgcctgt tcgaggacgt ggtcggcatc gaggatctcg
    12781 actaccaccc caagcccggc atccgcgcct accgcggcct gctccagcgc cggcgcctga
    12841 acgccgcgca atgcatcatg ctggaagatt cggccgtcaa cctgcgcacc gccaagcgcc
    12901 tcggcatgcg taccgtgctg gtgggcggcg ggctgcgcac gccggcctac gtggacctgc
    12961 gcatccccaa catcctcgcg ctgcgccgcc agctcgggcg gctgcaggtg cgctgagaag
    13021 aagaacgcgt gcggccagga gcgcgcgcga cggcgctcga ggctggccgg cacgtccgcg
    13081 ctggctcagt cgcgcagcgc acgctccatc aggcaggcgt cctcgccata gcccgccggc
    13141 agcaccgccg gcagcgcggc gaagccttgg cgtgcccaga acggcaccgc atcggcgagc
    13201 gccaccaggc gcagtccgcg atgccccgcg gcggccgacc agtcgcgcac gcgaccgaac
    13261 agcgctgccg ccacgcccag cccgcgcgcc gccggcacca ccgcgaggtc atgaacgaag
    13321 ccatgcccgg cgcgtgccgg caaggctcgc agcggcgcat gcaggggcgg cgcgtcggca
    13381 tcgtcccagg gatgggcgaa cacgtagccc accgccgcac cgtccgcctg cgcgagccag
    13441 cacgcctgcg gcgcgagttc gagcttgcgc ccgagcaccg cggccgactc ctggtagccg
    13501 tcgccgtagg ccgcgcgctg gatcgccagc acgtccggca ggtcggccgc gcacaacggc
    13561 cgcaggaccg gccgcgccgt catcaggcgc cggccttaag ccagcgcgcc gcgtcgagcg
    13621 cgaaataggt caggatgccg tcggcgccgg cgcgcttgaa ggacagcagg gcctcgagcg
    13681 cgcacgccct ctcgtccagc cagccgttgg ccgcggcggc cttgagcatg gcgtactcgc
    13741 cgctgacctg gtaggcgtag gtcggcacct gcagctcctg cttcacgcgg cgcacgatgt
    13801 cgaggtaggg catgccgggc ttcaccatga acatgtcggc accctcggcg atatcgagct
    13861 cgacctcgcg gagggcctcg tcgctgttgc ccgggtccat ctgataggtg tgcttgttgc
    13921 ccttgccgag gttgccggcc gagcccaccg cgtcgcggaa ggggccgtag aagctcgagg
    13981 cgtactttgc agagtaggcc aggatgcggg tgtggatgtg gcccgcgccg tccagctcgg
    14041 cgcggatgcg gccgatgcgg ccatccatca tgtcggaagg cgccacgaca tcggcgccgg
    14101 cctgggcgtg gcacagggcc tgcttggcga gcgcctccag ggtctcgtcg ttcatcacgt
    14161 agccgcgcgg gtcggcgggg tcgatgagac cgtcctggcc gtggctggtg tagggatcga
    14221 gcgccacgtc ggtgatcacg cccaggtcgg ggaagttcgc cttgagcgcc tgcacggtgc
    14281 gcggcaccaa gccctccgga ttccaggcct cctcggcgcc gggggtcttg ccggcgcggt
    14341 cgatcaccgg gaacagcgcg agcgccggca cgcccaggcg catcgcctcc tcggccacgc
    14401 ccaacagccg gtcgatgctc acacgctcga caccgggcat cgagggcacg gcctcgctgc
    14461 ggtcggtgcc gtcgagcacg aacaccggat agatcaggtc gttggcggtg agcacgtgct
    14521 cgcgcatcag acggcgggaa aattcgtcac ggcgcatgcg gcgcatgcgg gtcgcgggaa
    14581 aggctccagt gggtcgcatc tcggtctcta gcaggtcgtt gtggaacgtg cggcggggac
    14641 gtgcggcggc gggcgccgac cgcagggcgc gcgcggatca cctcacgcac ggccagcgca
    14701 accgcgcacg ccggcagggc cgtgcgcgcg gcttgcggaa ctttcacgtc aggagctcat
    14761 cttagcaggc agatggcgcc gtgcccccag gccggccatc tcccgcttca cctccctgag
    14821 cgggtgcctc acgcctggtg aggcattttc gcccccgact acgtcccctg gtcgggggct
    14881 tttgtttatg cgcccggcgg cgcatcgacc gcgccggcag gcgtgtcggc gaggtcgccg
    14941 cccaccgtgc cggctggcac cagccagccg ccgatcacgc gctcgacctc ctccaccccg
    15001 ctcttcttca ggctggaaaa cagctgcacg gtgacctgcg gcccgagcgg ctcgagcgcg
    15061 cgacgcacct cctgcagggt cgccgcggcg gcgccgcgcg agagcttgtc gctcttggtg
    15121 agcagcacgt gcatcggcct gcccgagggc gcgaaccaat cgatcatgtt gcggtcgaga
    15181 ggcgtgagcg gatggcggct gtccatgatc agcacgagtc cgatcaggct ctcgcgggta
    15241 cgcaggtagg tctcgatcag ccccgaccac tggcggcgga tcttctcggg caccgcggcg
    15301 tagccgtagc cgggcagatc gacgagcagc gcgccgttgt tgagacggaa gaagttgatc
    15361 agctgggtgc gcccgggcgt cttcgacacg taggccaggc gcacgtgacc ggcgagggtg
    15421 ttgatcgcgc tcgacttgcc ggcgttggag cgcccggcga aggcgatctc cgcgcccgag
    15481 ggcggtggca ggcccgaagg cttggcgatc gagatttcga atgttgcgtt gcggaacaga
    15541 ggcatggagg aagatcactg aaaaccgcgc cttgcgcggc gaacagacct catcgcatcg
    15601 cgcaatcagg cgttcgtgta ttagaatacc gcgtttgaaa caatcactca cggctttccg
    15661 aggacatcat gatcaagcgt tccctgctgc tctcgtcgct tctgctggtc gccggcggcc
    15721 ttcaggccca ggaccaggcc ccggacctcg aaaaggccaa gcagaccgcc gagacggtct
    15781 gcgcggcctg tcacagcgcc gacggcaaca gccagctccc ggccaacccc aagcttgccg
    15841 gccagcacga ggactacctc ttcaagcagc tgcgcgagtt caagggctgg aacggcaatc
    15901 cgccggtgcg tgagaacgcg atcatgggcg cgatggtcgc cggcctcgag gaagccgaca
    15961 tgcgcggcct cgccaagtac ttcgccacct tcgagcagaa gcctgagccg gccaagagcc
    16021 tcggcaccat cgacctcggc cagaagatct ggcgcgcggg catccccgcc aagggcgtcc
    16081 ccgcctgtgc cgcctgccac ggccccgccg gcgcgggcat gccggcgcaa tatccgcgcc
    16141 tgtccggcca gttcgccgaa tacaccgaag cgcagatgaa ggccttccgc gacggcgtgc
    16201 gcgcaaacga cccgaacggc atgatgcgca ccatcgcgct caagatgacc gatccggaga
    16261 tcaaggccgt cgccgactac gcgaccggcc tgcgctgacc ctgcgccagc cctgcgcagc
    16321 ccccaccgcc gagccgcgtg ggggcgggcg ggggcgatcg tctgcatcgc gcgcccgcgc
    16381 cgcacgccct gcgacgctgg cgtccgtctt tctccgaggt ccggatgcat agcggcatcg
    16441 cgcgcgccct cttcgagctc ttcagctcga tgcgttttgc catctccctg ctgacgatcc
    16501 tgtcgatcgc gtcggtagtg ggaacggtcg ttcgccagaa cgaacccttc aacgcctatc
    16561 tcaaccagtt cggtccgttc tggttcccgg tcttcgagaa gctcggcctg tactccgtgt
    16621 acaacgccgg ctggttcctc gtcatcctcg ccttcctggt gctgtcgacc acgctgtgca
    16681 tcgtgcgcca gaccgcgccg atgctgcgcg agatgcgcag cttccgcgag cgcgcgcgcg
    16741 aggcctcgct gcgcagcttc gcccaccgcg tcagcctcgt gcccgcgctg ccccccgccg
    16801 acgcggtgga gcgcgcgcag gcctacctcg cgcacgccgg cttcaccagc cgggtcgccg
    16861 acaacggcga aggcaccctg ctcgccgccc gccagggcag cgccggacgc atcggctact
    16921 tcctcgccca cggcgcgatc gtgctgatct gcatcggcgg cctgctcgac ggcaacctgc
    16981 cgctgcacct gcaggtgtgg ctgggcgaca agcacaccac cgcgggcgac caggtcatcg
    17041 cgaacattcc accgtcggcc cgtctcggca cgggcaaccc gagcttccgc ggcgacgtct
    17101 tcatccccga gggccgcagc accagcttcg cggtgctcgg cctggccgac ggcatcctgc
    17161 tgcaggaact gcccttcaac gtcacgctcg acaagttcca catcgagcac taccagaacg
    17221 gcatgcccaa gcgcttcgcc agcgacatcc gcatcaccga ccggtcgagc ggccagacca
    17281 cgcggcacac catcgaggtc aaccacccgc tcaccgtcga cggcatcacc ctctaccagt
    17341 cgagcttcga ggatggcggc tcgaagctgg cgatcaaggc gcgcagcctg ctgcctggcc
    17401 gtcccggggt gctgcgcgag atcgagggcg tggtgggcga aagcctggcg ttcgccgacg
    17461 gcgccgcggg cttctcctat acgctggaat tcaccgactt ccgggccttc aacgtcgagg
    17521 acatgcgcgg caacgagggc gggcaaggcg acgaaggcga ggccgcgccg cgcggcatgg
    17581 acaagctgca gcgccacctc ggcagcggcg ccaaaggcgc ggaggtgcgc gacctgcgca
    17641 acatcggccc cagcttcacc ttcaagctgc gcgaccccgc cggccaggcg cgcgagttcc
    17701 acaactacat gctgccgatc gagcagggcg ggcgctggtt cctctacacc ggcctgcgcg
    17761 acgcgcaggc cgacgccttc cgctacatgc gcatccccct cgacgagcgc ggcggagtgg
    17821 atgcctggtt cgcgatccgc gaccgcctgc tcgaccccgc gcagcgcgac gctctggtcg
    17881 cccgcttcgc cgcgcgcagc ttccccaccg gtcgcggcca ggacgcgatg cgcgaggccc
    17941 tggtgcagtc ggcccggcag accctcgggc tgttcgccga gaagggctac gagacactcg
    18001 gcggcttcat cgagaagtcc gtgcccgagg ccgaacgcgg cccggccgcc gacgtgctgg
    18061 tgcgcatcct gcaggggctc gcctgggagg catggatgct gcaccgcgag gccggtggcg
    18121 aaccgccgct gcagctcgac ccggcgcgcg ccaccttcgt caacgacacc atcgcggcga
    18181 tctccgacag ccagttctac ggcgcgcctg cctggcttca gctcgacggc ttcgaggagc
    18241 gtcacgccac cgtgctgcag gcgacgcgct cgccgggcat gccgctcgtc tatctgggcg
    18301 cgctgctgtt ggtgctgggc gtgttcgcga tgctctacat ccgcgaacgt cgcctgttcg
    18361 tgctggtgaa gccgaacgag gcgctggtcg cgatgtcttc caatcgcagg gcgctcgacg
    18421 tcgacgaggc cttccgccaa cacgtcgaag gcctcgccgc catgctcggc gcccccgtgc
    18481 ccgcatccga accccgagaa agtgcatgat ggaaatcgcc cgcccctccc ccgccacccc
    18541 ggccggtccg gcccccgccg cgctccggcc gcacacgccg ctgctgcgcc ggctcggcgc
    18601 cttcgactgg ctgtacgcgc tcgcgctgat gctgggcgcg gccttcgcgc tcgtccgcta
    18661 ccacccggtg atggacgtat acgacgatct catcctggtg ctgcaagtgc cggtgctcgc
    18721 cgtgatcggc tggcagtgga agcccttccg cgccttcctc gccgcggtgg cgctgctggc
    18781 gctggcgagc atcgcgctct accaaggcga tctcgcgcgc gccgagcaga ccttcctgct
    18841 caagtacctg atctccagcc agaccgcgat catgtggatg agcgcgctga tcttcatcgc
    18901 cacgggcgcg tactggctgg gcctgctcgc acgcagcgac ttcgccgagc gcaccggctc
    18961 ggcgctgacc tggatcgccg cggtcatggg caccaccggc ctcttcgtgc gctggtacga
    19021 gtcctacctg ctcggccccg agatcggcca catcccgatc agcaacctgt acgaggtctt
    19081 cgtgctgttc gcgctgatga cggcgctgct ctacctctac tacgagggtc gctacgccac
    19141 gcggcgcatg ggcgccttcg tgatgatcgt gatctcggcc gcggtggcct tcctgctctg
    19201 gtacaccttc acccgcgagg cgcacgagat ccagccgctg atcccggcgc tgcagtccta
    19261 ctggatgaag gtgcacgtgc cgaccaactt catcggctac ggcgccttcg cggtctcggc
    19321 catggtcggc accgcctacc tgctcgccga gcgcggcatc ctcgcctccc gcctgccgcc
    19381 cttgcgcgtg ctcgaggacg tgatgtacaa ggcgatcgcg atcggcttcg ccttcttcac
    19441 catcgccacc atcctcggcg cgctgtgggc ggccgaggcc tggggcacct actggcagtg
    19501 ggacccgaag gagacctggg cgctgatcgt gtggctcaac tacgcgacct ggctgcacat
    19561 gcgcctgacc aagggcctgc gcggcgcggt gctggcatgg tgggcggtgg tgggcctgct
    19621 cgtcaccacc ttcgccttca tcggcgtcaa catgttcctg accggcctgc actcctacgg
    19681 tgcgctgtga gcgcgccggg gctcgcagcg cggtcgccca tcgaggacgg acccgaagca
    19741 tgaatcccaa tcccgcaggc ggcgacgacc gcagcttcct cgccgtcgat gccgcgcgcg
    19801 ccgcgatcct cggcgcgctc accccgatcg ccgggcgcga gcgcgtcgcg gtacgcgccg
    19861 cgctcggccg cgtgctcgcc gaggacgtga tcgcgccgtg caacgtgccc gcgcacgaca
    19921 actccgcgat ggacggctac gcggtgcgcg ccgccgacct ctcggccgat ggcgagagcg
    19981 cgctcgcggt ggtcggcacc gccttcgcag gccggccctt ctcgggcatg gtcggcgccg
    20041 gccaggccgt gcgcatcatg accggcgcca ccatccctca gggcgccgac accatcgtcg
    20101 tgcaggaagt cgcccgtcgc gagcacgagc gcgtgttcat ccccatcggg cagaagaagg
    20161 gccagaacct gcgccgcgcc ggcgaggacc tcgccgaggg cggggtcgcg atccccgcgg
    20221 gcaagcgctg cggtcccgcc gaactcggcc tgatcgcctc gctcggcatc gccgaggtgt
    20281 gcgtgcgccg ccgcctgcgc gtggcggtga tgtccaccgg cgacgagatc gcctcgatcg
    20341 gccgcccgct cggccccggc gaggtctacg acagcaaccg ctacaccctg cacggcgtgc
    20401 tcacccgcct gggctgcgag ctcctcgacc tcggcgtcgt gccggaccgc ccggaggcgc
    20461 tcgaggaggc cttccgcgag gccgcccagg ccgccgacgt gatcatcacc agcggcggcg
    20521 tgtcggtcgg cgaggccgac ttcatccgcg agatgatgaa ccgcttgggg gaggtggcgt
    20581 tctggaagct cgcgatcaag cccgggcggc cgatggcctt cggccgcatc ggcgatgcgg
    20641 tgctgttcgg gctgcccggc aacccggtgg cggtgatggt caccttctac cagttcgtgc
    20701 aggacgcgct gctccagctc atgggcgtcg caccgctgcc cgcgcccgag cgcttccccg
    20761 tgcgcgccgc cttctcgatg cgcaagcagc cgggtcgcgt cgagtacctg cgcggccgcc
    20821 tgcagcgcag cggcgaggcg cttgccgtgg cgcccgccgg cgcccagggc tccggcgtgc
    20881 tgagctccat gtccgaggcc gactgcttcg tcgtgctgcc ggccgactgc accgccgtga
    20941 gagaaggcga caccgtcctc gtgcaaccct tccacggcat cgtctgagcg gccggcttcc
    21001 ccctccccct ttccccttcc cacctcatcc cttcctcgcg cacatgaccc aggacgaact
    21061 caagaaagcc gccgcctacg ccgctctcga ccacgtggtc gaaggcagca tcgtcggcgt
    21121 cggcaccggc tccaccgtca accacttcat cgatgcgctc gccggcatgc gcgatcgcat
    21181 ccgcggcgcg gtctccagct ccgaagcgag cacgcaacgc ctgcgcgcgc acggcatcga
    21241 ggtgttcgac ctcgacggca tcgagtcgct gccggtgtat gtagacggcg ccgacgagat
    21301 cgacggcggc ttcgcgatga tcaagggcgg cggcggcgca ctgacccgcg agaagatcgt
    21361 cgccgcagtg tccgagcgct tcgtgtgcat ctgcgacgcg agcaagaagg tcgaggtgct
    21421 cggccgcttc ccgctgccga tcgaggtgat cccgatggcc cgcagccacg tcgcccgcga
    21481 gctgcgcgcg ctcggcggcg agcccaggct gcgcgagggc ttcgtcaccg acaacggcaa
    21541 cctgatcctc gacgtgcatg ggctgtcgat caccgacccc gtcaccatcg agatgcggat
    21601 caaccagatc gtcggtgtgg tcaccaacgg gctcttcgcc gtgcgcggcg ccgacgtgct
    21661 gctgctcgcc gcccccggcg gcgtgcaacg gctggacgca cggtgagcga cctgcatggc
    21721 agccgtgttg caggcaacaa aaccttaaca atcgcctgtt agcctgcgcg cttcccggac
    21781 tggatcgtgg cgaacatgac agaacacact ctcagacagt acgacatcga actcgaagaa
    21841 atccgccgtg gcgtcctcaa catgggcggc ctggcggagg cgcaggtcgt caaggcgatc
    21901 gagggcctgc gcagcggcca gctcgacctg ctcgacaccg tgatcgaagg cgacaagccg
    21961 atcaacctcg cgcaggtcga gctcgacgac gactgcagcc acatcatcgc caagcgccag
    22021 ccggccgccg gcgacctgcg cctggtgctg gcggtgatca agatcgcctc cgacctcgag
    22081 cgcatcggcg acgaggcgaa gaagatcgcc aaggcggcgc gccgcctgca cagcgccgaa
    22141 acccccttcg tgccgcgcgt gggcctcacc caggcggtgg agctcgcgct cgaactgctg
    22201 cgcgcctcgc ttgacgcctt cgcgcgggtg aatccggaag gcatcgagga cctgcgccgc
    22261 aaggacgcgg agatcgacgc cggcttcaag ggcgtgatgc gccagctcat cacctacatg
    22321 atggaagacc cgcgcacgat ctcgagctgc ctcgagatgc tcttcatcgc caaggcgatc
    22381 gagcgcgtcg gcgaccacgc gatgaacatc gccgagcacg tggtgttcgt ggcgcgcggc
    22441 gaggacgtgc gcttcgagaa gtcgcaggcg cagctcggca ggggctgagg cgtcaggcat
    22501 gaaaaagggg agcttgcggc tccccttttt tacatcggcg atccgggctt acgcctgcgg
    22561 cgggacgtag ccggccacct ggtcggcgcc accttcgaag aagtgcttct ccatctgctc
    22621 ggcgaggtac ttgcgcgcgc gcgcgtccat caggttcagg cggttctcgt tgatcagcat
    22681 ggtctggtac ttgacccact gctgccaggc ttccttcgag acgttctcga agatgcgctt
    22741 gccgagcgcg ccgggcaccg gtggcaggtc caggccttcg gcctcgcgac cgagtttcac
    22801 gcagttcacc atgcgagcca ttgttcaatc ctctctgctt gcgggttcat cggggttcga
    22861 ggacggcatt ctagccgatt cgtcggcccg cctcccggac accgcggcgc gattgcgccc
    22921 ctccgacttg gcgcgataga gcgcctcgtc ggcggcggcg accagggcct cgccctcgcg
    22981 cgcgttgtcg ggaaagcgcg cgatgcccac gctggcggtg acgcgcgccg cgcggccctc
    23041 gaagacgaag cgcatcgcgc ccaccgtctc caccaggcgg tgggcgagtt cgcacaggcc
    23101 ctccgtgctg ccctcgggca cgagcagggc gaactcgtcg ccgccgatgc ggaacaaggt
    23161 ctcgttgcgg cgcacggtgc gcccgagttc ccgcgcgagg cgaacgagca cctcgtcgcc
    23221 ggcctggtgg ccgaaggagt cgttgatcgg cttgaaaccg tccagatcga aggcgaggag
    23281 gccgagccgg ctgccgcgac gcccggcgtc cgcgagcatg cgaccgagtt cttcatggaa
    23341 gcgacggcga ttgtagagac cggtgagttc gtcgcgttcg gcgagctcga cgagctgcat
    23401 cgaggtgcgc cgcgccacgg tcacgtcctc gtagatccac agccggccga tgcgccgccc
    23461 ggcttccccg cccgcgacca ccgccgaacg gtcggtggcg atgcgcccgt cggcgaaccg
    23521 gaactcgtac tcttcgctga tcgactgctt ggcccgcacg gtgcgatgga tgtgggcgag
    23581 atagcccgcc ggatcggcga gcacgaccgg cgcacgctcc ttgagcaccg cgtcgcggca
    23641 gccgacgagc acggtgtcgg ggggatagcc ccagatcgcc agcatcgcac ggttgtggta
    23701 gagcacgcgg cggtcgtggt cgatgaacag gatgccgagc cggatcaggt tcagcagcgc
    23761 agccaggcgc tggcgctcgt cgttgctgcg cacgaggtag agctcgcgca gcgcctcctg
    23821 gcggcgcagc tcggccaccg tctcaagcag ggtgaattcg gtctccttgc gcgcctggat
    23881 gtgctcggcc gacaggcgca ggccgccgtc ggcgcgcggc cgccagtgcg tctgcaccca
    23941 aaccgcccgc ccatcccgtg cgcgcaggcg cagctcgaag gcctgggcgg attcctccgc
    24001 agcgacccgc tgcgccatcc gccggcagaa cacgcgatcg tcctcgaaca ccaggagttc
    24061 gaaggggtcg gccgccgcga cgcaatccgc cgccgtgaag ccggtaagcg cctcgatcga
    24121 ggggctgatc cagcgcagcg cgagcccggg caggaagatc gcctcgacgc tgcgcacgcc
    24181 gagcgcgatc tcggcgagca gttcggcgtc ctccgccttg ccgggtgccg ggcaggcgct
    24241 catagcggct tgacgaacac cttcgagcga cgctggaagt tgtagagttc gcgcttctgc
    24301 cgcggcaggg cctcggggcc gatctcgctg aagccgcgct cgcggaacca gtgcgcggtg
    24361 cgggtcgtga gcacgaacag gcgctcgagc cgctgcacgc gcgcgcgccg ctcgatgcgc
    24421 ttgaggagct ggtcgccgag accggcgcgg cgatactccg gcgtcaccgc caggcaggcg
    24481 agctcggcgg cgttgtcctc gctgaacgga tacagcgccg cgcagcccac cagcacgccg
    24541 tcgtgctcga cgacggtgaa gcgctcgatc tcctgctcca gcagctcgcg cccgcgccgc
    24601 accagggtgc cgtcggcctc catcggcgag atcagcgcga cgagagcggc gacatcctcg
    24661 accgtggcct cgcgcaggcg gaacagcggg tcgcgcgaca ccaccgtgcc gacgcccgca
    24721 tgcgtgaaga actcgagcag caggccgccg tcgaggtcgt gatcgatcag gtggcagcgc
    24781 gccaccccct tcttcaccgc acggatcgcg cacggcagga acaggtgcag gtcttcggtg
    24841 agcccctcgc ccgtctcgag cttgccctgc gcctcgtcgg cggtgaccga ctcgacgagc
    24901 tggccatcct cgccgagcag gccgggcgca tcgcacaggt agatcagctt ctcggccttg
    24961 agcgccaccg ccaccgcctc ggccacctcc tccatcgcga gattgaagat ctcgcccgcg
    25021 ggcgagacgc cgagcggggt gatcagcacc acgttctgct ggtcgaggtc cgcgttgatc
    25081 tcctcggcga tgatcttgcg caccgcgccc gtgtattggt aatcgacgcc atcgaccacg
    25141 cccaccggcc gcgcggtgat gaagttgccc ccggtgacgc ggatgtagct gcccgccatc
    25201 ggggtgttgg gcaggccctg cgagagcagc gcctcgacct cgaagcgggt caccgccatc
    25261 gccgacttga cgcattcgag cgcatcggcg tcggtcacgc gcaggccgtg gtggaaacgc
    25321 ggctcgagcc cgcggcgggc gagctcggca tcgatctgcg gtcgcgcgcc atgcaccagc
    25381 accaggcgga tgccgagcgc ggcgagcagg ttgcagtcgt aggcgaggct ttgtgcgcgc
    25441 ttgccggcgg cgacctcgcc gccgaagccg acgacgaagg tgcggccgcg gaaggcgtgg
    25501 atgtagggcg ccgcgccgcg cacccaggcc acgaactggg ccgtggcgtc atccacgcgt
    25561 ggaacggccg gggaaggaga ggaggtttcg ggactggaac tgctgttttc ggcgctcacg
    25621 cattcaccgc tgttggggcc ggacacacat ggggcgaccc ggcggactgg aatcgacgac
    25681 ggattctagc ccggccgggc accggcgtgg aacgcgggcg cgcgcgttca gcccttgtac
    25741 ggtcccgccc ccaagccgat cggcgccggc gcggcggcga cgagcctgga gaagatctgc
    25801 gcgaagtcct cctcgcccgc ccgggcactg gcgcgcgtgc cgccgagggg atcgtcggcg
    25861 atgaagccgg cgttggcgcg cgcccagtcc tctgcggtga agtgcttgcg gatctgcggc
    25921 agcacctcgc gctcctcggt catcatgtgg ttgcgataga acgcggcgta gtcgttgaag
    25981 gcggtcttga aggcctcgaa gccgccgacg gggtcctgcg cgtagccctt gaccgcggcc
    26041 tcgagcaccg cgatgcgggc gtcggcctga gcgtgttcgg cctcgagccg gtcgagcgcg
    26101 gcgtccgcgt cgtgcgtgcg ggcacgcagc ggcccgaaca ggaaggcatc ttccttgggg
    26161 tggtggcgct tctcggggta ggcgtcgagg taatgcacca tcgcctcgag cagcttgtga
    26221 tccgggcgca ggcggccggc atcgatctcg ccgatcatgt gccggatggc gtggatgatc
    26281 gccgccagcg actggtgttc gtccatcagg atgcgcatcg cgtccatggt cttgtccttt
    26341 tccgtgggga gctgagggct ggtggctcag gtcgacagca tcgcctggtg cttgccgccg
    26401 agcccgaggt aggcctcgat cacgcgcgga tcgtccgcga gctcgtgcgc cgggccctgc
    26461 atggcgatct ggcccgtctc gagcacgtag gcgtagtcgg ccacctgcag cgcggcgcgc
    26521 gcgttctgct cgacgagcag gatcgacacc ccgcgccggc gcagctcgct gatgatgcgg
    26581 aagatctcgc gcacgatcag cggcgccagc cccaggctgg gctcgtcgag catcagcagc
    26641 ttgggcttgg ccatcagcgc gcggcccacc gcaagcatct ggcgctcgcc gcccgacatg
    26701 gtgcctgcgg cctggctgcg gcgctccttg aggcgcggga agagcgcata gacctcctcg
    26761 agcgtctgcg cgtgatcgcg cttgcccatg cgatagcgct ggaaggcgcc cagcaccagg
    26821 ttgtcctcga cgctcatctc gccgaagagc tcgcgcttct cgggcaccag ggtcatgccg
    26881 cgcgcgacca tgcgttcgac ctcgggcacg ccttccacgc tgccgtcgaa ggcgacctgg
    26941 ccgcgcgagc cgagcacgcc catgatcgcc gacagcatcg tcgtcttgcc cgcaccgttg
    27001 gggccgatca cggtgacgat ctggccctcg ccgacggtga ggctggcatc ggtgagcgcc
    27061 tcgaccttgc cgtaggacac gcacaggtcc ttgacctcga gcaccgggtt cttcgcgtcc
    27121 gcgttcgagg gtgcggcgct gccttgcgtc tgcttattca tcgcgttctc cccgctcatt
    27181 ccacaccgcc cagataggcc tcgagcaccg ccgggttctg ctggatctcg tcgggcaggc
    27241 cctcggcgat cttctggccg aactccatca ccaccacgcg atcgaccagg ctcatcacga
    27301 agtccatgtc gtgctccacg atcagggtcg ccatgccctc cgcgcgcagc ttccggagca
    27361 actcgccgag cgcctccttc tccctgaagc gcaggccggc cgcgggctcg tcgagcagca
    27421 gcaggcaggg gtcggcgcac agcgcacgcg cgatctccag gatgcgctgc tggcccaggg
    27481 cgaggctgcc ggcctcctcg tacatgtact cggccaggcc cacgcgctcg atctgacggg
    27541 cggcttcctt gaggatgcgg gcctcttccg cgcggtccag gcgccaggcc gagctcaaca
    27601 cgcccttgtc gctcctgagg tgggcgccga tcgccacgtt ctcgagcacg ctcatcgtcg
    27661 gcagcagctt cacgtgctgg aaggtacggc tcatgcccat gcgggcgatc tcgcgcgagt
    27721 cgtggccggc caccggcttg ccgaggaagc gcacctcgcc cgaggtcggg gtgtcgacgc
    27781 ccgagatctg gttgaacatc gtgctcttgc ccgcgccgtt ggggccgatg agcgcgagga
    27841 tctcgcctgc gcgcacctcc aggctcatgt tgttgttggc gatcaggccg ccgaaccggc
    27901 gcgtgacgtc cttggcctcg aggatcagtt cgcccgcctt cggcagggtg cgccgcggca
    27961 gcggctcggc gtcggggtcg atggtcttgc ggagcgcacg caccggcacc agtttcttga
    28021 ggatcggcca caggccctcg cgggcgcggt gcaacaccag caccatcagc aggccgaaca
    28081 cgatggtctc gaagttgccg gtggcgccga gcaggctggg cagccagttc tgcagccact
    28141 gcttgagcac ggtgatgacg cccgcgccca cgatcgcccc ccacacgtag ccggcgccgc
    28201 cgaccaccgc catgaagagg tactcgatgc cgatatgcac gccgaacggg gtcgggttca
    28261 cgaagcgctg catgtgcgca tacagccagc ccgaggcgca cgccagcagc gccgcgatca
    28321 cgaagatcac catgcgcgag cgcgaggtgt ccacgcccat cgcctctgcc atcacgcggc
    28381 cgcccttgag ggcgcggatc gcgcgcccct cgcgcgagtc gagcaggttg tgggtggtga
    28441 gcatggcgac gagcaggaag gcccagatca ggtaatagat ctccccgccc tcgtccagcg
    28501 tccagcccag cagcccgatg gccggaatgc cgctgatgcc ggtgtggccg cccaggctct
    28561 ccatggtgcc gaagaggaag tacaggctga tgccccacgc gatcgtgccc aggggcagga
    28621 agtgccccga gagcttcagg gtgagcgagc cgatcagcac cgcgaccagg gcggtcagca
    28681 gcagcccgac cgccagggtg agccagggcg aagtccccgc ccaggcgagc catcccggca
    28741 ggtcggtcgc ggtggtgagc gccgcggtgg tgtaggcgcc cagacccacg aaggcggcct
    28801 ggccgaagct ggtcagtccg cccaccccgg tgagcagcac caggccgagc gcaaccatcg
    28861 catacaggcc gatgtagttc agcagcgtca cgtaatacgg cgacaggatc gccggcacca
    28921 gcagcagcag ggcgagaaag gcccccagga ccagacggtc ggttctcatt cttcttcctc
    28981 cacgtggtgg ctacgcagcg agcgccacag cagcacggga atgatcaggg tgaacacgat
    29041 gacttccttg taggcgctgg cccagaacga cgcgaaggct tcgagcagcc ccacggcgag
    29101 cgcacccacg gcggccagcg gataactcgc caggccgccg atgatggcgg cgacgaaccc
    29161 cttgaggccg atcaggaagc cggtgtcgta gtagatcgtg gtgatcgggg cgatcagcac
    29221 gcccgaaagc gcaccgatga aggcggcgag gaagaacgtc agcttgccgg cgagcgtggg
    29281 cgagatcccc atgagctgtg caccgacgcg gttgatcgcg gtggcgcgca aggccttgcc
    29341 gtagagcgtg cgttcgaaga actggtacag cgccacgatc agcagcaccg acaccaccac
    29401 cacccacagc gtctgcgcgg agaccatcag cgggccgagc tcgaggctga actcgctgaa
    29461 cgccggcgtg cgctggcctt ccgctccgaa gaacagcagt cccaggccca ccatcgcgat
    29521 atgcagcgcc accgagacga tcaggagcac cagcaccggc gccgatgcga tcggctggta
    29581 catcaggcgg tacatcatcg gccccatcgg caccacgatc gccagcgtca gcagcacctg
    29641 caccgccagc ggcagctcgt tgaccgccag cccgccgacc accgcggcga gcgccagcgg
    29701 atagcccagg ttccagcccg ccacgctcgc ggcacgccgg ccctgccccg ccttgagcgc
    29761 gagcccgccg tcgagcaccg tgaccagcgc tcccatcgcc accagcatcc acaccgtggc
    29821 cggcaacacg cccgactgca tcatcgccag cgtcagcgcc ccgtaggcca cgaactcgcc
    29881 ctgctggatg aagatcaccc gcgtgaccgc gaacaccagc accagcgcca gcgccagcag
    29941 cgcgtagatc gcgccgttgg tgatcccgtc ctgcgacagg atcaatgcaa ttgacatatc
    30001 catggaaatg cctcgtctcg gcggtgcctc gcggaccgcc gtgattgatt cgatgtgtcg
    30061 gaaacccccg acagacgcgc ggcgggacgc caggcgcccc gccgcggagg gggatgctta
    30121 cttcagcagc gaccaggtgc cgttgcggat ctcgatcatc acgcgcgagc ggtcgtccag
    30181 gccgaggtga tcgtcggggc tcatcgtgta gatgccggtg gcgccggcga cttccttcac
    30241 gccctcgagc gcatcgcgca gcgccgtgcg gaactccgcc gagcccggct tggccgcctt
    30301 gagcgcggca gggacggccg cctgcagcaa caccccggca tcccacgcgt aggccccgaa
    30361 gctcgacacc gagcccgcgc cgttggcggc ctcgtacttc tccacgtagg ccagcgcgct
    30421 cgccttgacg gggtggctgt cgggcaggct gcgcgccatc tgcaccgggc cgaccggcag
    30481 cagcgtgcct tcgcagtcct tgccgcagat gcgcaggaag tcgttgttgg ccacgccgtg
    30541 ggtctggtag atcggcccct tgtagccgcg ctcgcgcagc gtcttctgcg gcagcgccga
    30601 gggcgtgccc gagccggcga tgaacaccgc gtccggcttc accgacatca gcttgagcgc
    30661 ctggccggtc accgaggtgt cggccgggtt gaagcgctcg ctggcggcga tctcgatgcc
    30721 cttggcttcg gccagcttct tgaactgctc gtaccagccc tcgccgtagg cgttggcgaa
    30781 gccgatgaag gacaccttcg tcaccttgtt ggcgaccatg tgctcgacga tcgccgacgc
    30841 catctgctgg tcgttctggg gggtcttgaa gacccagcgc ttcttgtcgt ccatcggcgc
    30901 gacgatgcgc gccgacgcgg ccatcgagat catcggcgtc ttcgtctcgg cggcgacgtc
    30961 gaccatggcc agcgaagcgg ggctggtggt ggagccgatg atcacatcga cgccgtcctc
    31021 gacggtcagc ttgcgggcgt tcttgaccgc cgtggtggtg tcggaggcgt cgtcgagcac
    31081 gatgtagctc accttttcgc cgccgatggt ggtcggcagc agcgcgatcg tgttcttttc
    31141 cgggataccc agcgaagcgg cggggccggt ggccgagagc acgacaccga ccttgatctc
    31201 ggcatgggcg gcagtggcgg cgaaggcgag gccgagggcg gccagcaggg tgttacgcaa
    31261 cttcatggtg tctcctccag tggttgtgcg gtgcgagggg gccggcgcgc cgggggcgca
    31321 gcgctcgggg cgcgcaagtt ctgcattgca gaacgcgttc tagttattag aattgataca
    31381 aaagatcccg tcaagcagaa actttgtatc aattccaaaa gtatccggct cctgctgtgc
    31441 gcaacctccc gccgcaccca cccctcaggt cgcgtggtcg cccgactcga cacccgggag
    31501 aagggtcgcg ccgagtcgcc gcgacagcgc gtcgcaggcc tccttcagcg cctggagcac
    31561 cgggctgtgc gggaggagat ccagcgtgcc ctcgacgccc acgagggcga tgctcatgtt
    31621 gatctcgttc ttgaagttga acaccggggc cgccgcagcc tcgacgccgc gggcgaaata
    31681 gccgctggaa atcaccgcca caccccgcgc gcggacctcg gcgagcagcc ggcgcgcgtc
    31741 gtcaagggtg gcaatgcccg cgggcacccg accggattcg agctcggcgc gcagcagcgc
    31801 ttcggtctgc tgctcgggca gccatgcggc gaagacgcgc ccggcggccg agctcagcag
    31861 gctgaggctg gtgccggtca gcacattgac ggtgatcgga cgacggggac gctcccagcg
    31921 cacgacgacc ggccctcgat cgctccagac ggcgagcgcg acggtctcgt tggtgagatc
    31981 gcgcaaatcg ccgatcgcgc tcaagccgag acggatccgg tcgacgcggt cgagggcgac
    32041 caggccgagg ttgagggcga agggaccgag gtcgtagcgc gaggtgagcc ggtcctgttc
    32101 gacgagtccg ctgcggatca ggctgaccag gtagcggtgc gccttcgagg gcggcatgtc
    32161 ggcggcctgg gcgatgtcct tgagcatcat ggagcgcttg ccggtgacca tggcgcggag
    32221 gatgctcatg cccacctcga gagattgcac gccgtgcttg ccgtcgagtt cgtcgctcat
    32281 cgaagccccc tcaccgataa aattacttat tgaagaaatc tttcctttat atgtaacata
    32341 agtccgagcg gaggagaaca aggatgaatc agctgaaagt ctatgcgatc gacggcatcg
    32401 tgccggtggt ggatccgacc gcctacgtgc acccgtcggc cgtgctgatc ggcgacgtga
    32461 tcgtgggccc ggactgctac gtcgggcctt gcgcaagcct gcgcggcgac ttcgggcgac
    32521 tgatcctgga gcgcggctcg aacctgcagg acacctgcgt gatgcatggc tttcccggca
    32581 ccgatacggt ggtcgaggag gacggacaca tcggccacgg cgccgtgctg cacggctgca
    32641 gggtgggccg caacgccttg gtcggcatga acgccgtgat catggacaac gcggtgatcg
    32701 gcgagtcatc gatcgtcgcc gcggcggcct tcgtcaaggc cggcgtcgag atgccgccgc
    32761 gcatgttgat cgccggcatg ccggccaagg cgatccgcgc gctctccgag gaggagatgc
    32821 gctggaagag ctcgggaacc gcgacctacc aggacctcac ccgccgcagc ctggccacga
    32881 tggaagagac cgtgccgctc gcggcggtcg agcccgggcg caagcgcatc cacatgccgg
    32941 acgtggtgcc gctggtggaa ctgcgcaggc gggaaggctg aggccggcgg cgcacgcagg
    33001 gcggcacggc catgaacgcg tcccgcacgc ccccgccccg gtcgccgcgc gccgaccggc
    33061 gggaagaggc tggcggcgct caggcggcgc cgcgcagcgg gtcggcgtcc tggaagcgcg
    33121 cgagaaggcg gggcgacgcc tcgggcgtgc ggccgtcggc gaggcggagg tgagcgtcga
    33181 tatggcgctc ggacggcacc agcagccgac ggtagagttc gcggcacagc agccgcgcac
    33241 gctgccccgg ccagtcgctc gccagcagca cgtcgggcaa tccgggatcg cgcagcagca
    33301 ggcggcggta gtcgtggatc agcagcatcc gcatgaggaa ggcgttctca tccccggact
    33361 cgctggcgcc atcgcggagc tgggccagca cgggctggta gcagtcgacg aattcgcgat
    33421 aggtctgcgc caggcgctcg agattccatg ccgcatgcac caggtcggcg tcgctggccg
    33481 ccgcacccag ggccgcatcc gcagccatca ggggcttgag ccgcggcagc agcccaccca
    33541 acccctcccc gacgagcgcg tcgaaggcgg tgatgaggtc cgcgctcgga tgcacgaagc
    33601 agtccccgtc gagggcgccg aagccctgcc agaacagcgc cctgcgcagg cgttcgcggt
    33661 ccttggccgc aagctcgccg acggcgagga tcagccgcca gcgacgatcc cagccctgcg
    33721 cagcggaagc gtagatgcgg cgcgacgcct cctcgatacg ctgcagaccg gaaggcgtca
    33781 gcatatagtc cgtacggcgg ccgcaaggct cgctgaccag ccattcatcg cgcgcaagcc
    33841 gaaacaccga tgtgcggacg agacgctcgt tgagcccaag cggctcgagc aagcggatca
    33901 ggctgcccag ccagatgcgt ccgcctcggg gcagcacggc gtcgccgaag acggaaatga
    33961 tcagcgaccc ggcctgcacg cgatcttgct tgcggaacgc ttcaaggcga tcttggatgg
    34021 aagtggtcac ggacgagctt gttcgcgctg cggttggggg ccagaagtct atcgattccg
    34081 ggcagatgcg cctagcgccg gagcgcgctt tcgaggccgc cgcactcgcg tacgcaaggc
    34141 tcgaggacga gcgccacgcc gattccgcgg gcacgaagcc tggatgcgtg cgggcgagtg
    34201 cgcgggaggg caaggcagca aggagattaa tacgatactt atactagatt tttctatgtc
    34261 ttcttgtatc acattaagtc cagagcagcg gacgccccgg gtttttcgac tggctacggc
    34321 tgccggaaca ccccgataat tttcacatat ccaactgttt ataaaagact tccttatatc
    34381 taaacgtgtt tcgcctatca aaacttgagg tgatcacccc ctcaagaata gatacctagt
    34441 attgacattt atcaaatcga tttgtatcgt ttgaagcaca agaccaccca ctcggagaga
    34501 cccatgcccc accccttgct cgaaaagcac cgcgccaccc tcgaaagcgc cctcgacgcc
    34561 atcgccacgc gcggctactg gtctgccttc cccgagatgc cgagccccaa gctctatggc
    34621 gaggcggcgc cggacgaggg caagcgcgcc ttcgaggcgc acctgggcaa gccgttcgag
    34681 ctcgggcagc cgggccagac gggctggcat ggcggcgaga gctcgcccta tggcgtggcg
    34741 ctcgacgtgc gctatccggt gtgcgacccg gagacgctga tcgccgccgg gctcgaggcg
    34801 atgaagggct ggcaggcggt gggcgccgac ggccgcaccg gcatctgcct ggaaatcctc
    34861 cagcgcctga acaagcagag ctttgagatc gcccacgcgg tcatgatgac caccggccag
    34921 ggctggatga tggccttcca ggccggcggc ccgcacgccc aggatcgcgg cctcgaggcc
    34981 gtgacctacg cctggcgcga gcagagcttc gtgccggccg agaccacctg ggagaagccc
    35041 cagggcaaga acccggcgct ggtcatgaag aagcacttcc agatcgtcgg ccgcggcgtg
    35101 ggcctggtga tcggctgcgg caccttcccg acctggaaca cctacccggg cctgttcgcc
    35161 gcgctcgcca ccggcaacgc ggtcatcgtc aaggcgcaca gcaatgccat cctgccggcg
    35221 gcgatcaccg tgcgcacgat ccgcaccgtg ctcgccgaga acggcatcga ccccaacctg
    35281 gtgagcctgt gcgtggccac ccagcgcagc gtcacccagg cgctcgccac ccacccggcg
    35341 gtgcagtcgg tcgacttcac cggcagcaac gtgttcggcc agtggctgat cgacaactgc
    35401 cgccaggccc aggtctatgc agagctcgcc ggcgtgaaca acatcgtcat cgactcgacg
    35461 gactcctaca aggcgatgct gggcaacctc gccttcacgc tctcgctgta ttccggccag
    35521 atgtgcacca cctcgcaggc gatcttcgtg cccgcgggcg ggatcgacac cgaggacggc
    35581 cacaagagct acgacgaggt ctgcgccgac ctcgcccgtg cggtcgagcg cttcctgtcc
    35641 aaacccgagg tcgcccacgc cgtgctcggc gcgatccagt ccgccgacac cgccgaacgc
    35701 atcgacatcg ccaacagcgg cgcgctgggc aaggtcgtgc tggcctcgca gaagctcgac
    35761 aaccccgagt tcccgggcgc caaggtgcgc accccggtgc tgctcgcctg cgacgccgcc
    35821 gacgagaagg cctacatgga agagcgcttc ggcccgatca gcttcatcgt caaggtcgcc
    35881 gacaccgccg cagggatcgc gctctccgag cgtgtggtca ggacccacgg cgcgctcacc
    35941 gtcgggctct actccacgag gcaggacgtc atcgacgcga tgaccgaagc cacctggcgc
    36001 ggcaaggtcg cgctgtcgat caacctcacc ggcggcgtgt tcgtgaacca gtcggcggcc
    36061 ttctccgatt accacggcac cggcggcaac ccgtcggcca acgcctcgta ttcggattcc
    36121 gccttcgtcg ccaaccgctt ccgcgtcgtc cagcgccgct accacgtctg aggatccgtc
    36181 cgtggaatac aacaacatcc agtttgcagt ggaggcgggc gtcgcccgcc tgacgctgaa
    36241 ccgccccgac aagctcaaca gcttcaccgg cgagatgcac gccgagctgc gcgacgcgct
    36301 cgaccgcgtc cacgccgaca aggccgcgcg cgtgctggtg ctcaccggcg ccggccgcgc
    36361 cttctgcgcc ggccaggatc tggccgatcc cgacatggcg tcggtgggcg gcaagatgcc
    36421 cgacatcggc aacgtcgtcg aaaagaacta caagccgctc gtgctgcgcc tgcagaacct
    36481 gcgcgtgccc accatcgccg cggtcaacgg catcgccgcc ggcgccggcg ccagcgtggc
    36541 gctcgcctgc gatctggtgg tggcggcgaa gtcggcctcc ttcctgcagg ccttcagcaa
    36601 gatcggactc gtacccgaca ccggcggcac ctggttcctg ccgcagcgcg tcggcatggc
    36661 gcgcgcgatg ggcctggcga tgctcgccga caagctgccc gccgagaagg ccgccgactg
    36721 gggcctgatc tgggcggcgt acgacgatgc cgagttcgct gcaaaggtgg acgcgctcgc
    36781 cgcgcagctc gccgccatgc cgaccaaggc cctggtgcgc acccgccagg ccatgcacgc
    36841 cgcggccggc catacgctgg agcagcagct ctccttcgag ggcggcttca tgcgcgagct
    36901 gggctggagc cccgactacg ccgaaggcgt cgccgccttc atggaaaagc gcgcaccgaa
    36961 gttcaccggg gaatgagcgt gaccgacacc cagatctcca agtccgccgc gcagccgctg
    37021 cccgcctcgg ctgtcgtcgc cgtggtcggc accggcgcca tgggcgccgg catcgcccag
    37081 gtggcggccg cggccggcca cgtggtcaag ctgctcgaca accgtccggg cgccgccgcc
    37141 aaggcgatcg aaggcatccg tgcccagttc ggcaagctcg ccgacaaggg caagatgagc
    37201 accgaagccg cgcaggcggc cgctgaacgc ctgatcgccg tcgaccagct ggccgagctc
    37261 gccgacgccg cactggtggt cgaggccatc gtcgagaacc tcgaagccaa gcagaagctg
    37321 tacggcgacc tggagaacat cgtcggcgcc gactgcatct tcggcaccaa cacctcgtcg
    37381 atctcggtga cctcgatcgg cgccgccctg cagcgccccg aacgcctggc cggcctgcac
    37441 ttcttcaacc cggcgccgct gatgaagctg gtcgagatcg tctccggcct cgccaccgcg
    37501 cgcgtggtcg ccgacaccct gttcgccacc gccgcggcct ggggcaagac gccggtgcat
    37561 gcgcgctcga cgccgggctt catcgtcaac cgcgtcgccc gtccctacta cgccgaggcg
    37621 ctgcgcatcc tcaacgaagg cggtggcgac tgcgccacac tcgacgcgat catgcgcgac
    37681 gccggcggtt tccgcatggg gccgttcgag ctgatggaca tgatcggcca cgacgtcaat
    37741 ttcgccgtca cacgctcggt gtggaacgcc ttctacaacg atgcgcgctt cacgccctcg
    37801 ctgatccagc aggagctggt cgacgccggc tttttcggtc gcaagtcggg ccgcggcttc
    37861 tacgactacc gcgagggcgc cgcgcgcccc gcaccccagg tcgcggtggc gcagcacgca
    37921 ccggacgaga tcgtgctgca cggccattcg gccgccgtgc atgcgctcgc cgaccgcctc
    37981 ttcgagcgca acatccccca tacctggggc gaagccaacg acggtcgcat cgccgagacg
    38041 gggggcgcgg tgatcttcgt caccgacggc cgcaccgcca cgcaacgcgc caccgagatg
    38101 ggcatcgcca acgtggtgct gatcgacctc gcgctggact acgccaccgc cagccgcatc
    38161 gcggtcggcg ccgcgatcaa ctgcttcgcg cccgcggtcg gcgccgcgat cagcctgctg
    38221 caggcggccg gcttcgaggt ctgccgcctg ggcgacaccc cgggactggc agtgatgcgc
    38281 accgtcgcga tgctcgccaa cgaagcggcc gacgcggtca accagggggt gtgcgatgcc
    38341 gccggcgccg atgcggcgat gaagctcggc gtcaattacc cgcgcggacc gctggaatgg
    38401 gccgaccgcg tcggcctgcc ggcgatctgc gccgtactcg ccaacctcgc ccgcttctac
    38461 ggcgaggacc gctatcgcgt gtccccgctg atccagcagc gcgtgttcgc cggaaagaag
    38521 atccatgact gatgcgcagc ccatcccgac cgatccgcag gccctggccg aggccgtgac
    38581 cgcggcgatg tggtcgcgcg accgtaccgc ccaggcgctc ggcatgcgca tcgagcacgt
    38641 cggtcccggc cgctccaggg tcagcatgac ggtgcgcggc gacatggtga acggccacca
    38701 catctgccac ggcggcctca tcttcaccct ggccgacacc gccttcgcct acgcctgcaa
    38761 cgcctacaac cgcaacaccg tggcgtccgg atgcaacatc gacttcgtcg ccaccgggcg
    38821 cgaaggcgac accttgcagg ccgaggccgt ggagcgcacg ctggccggtc gcaccggcgt
    38881 ctatgacgtc accgtgcgcg acagcgcagg caacaccatc gccctgttcc ggggcaagag
    38941 ctaccgtatc tccggcgagg tgatcgccgg actcgccgca gagaacgcct agacgggtcc
    39001 gcgcggcccc aagcacacgt agagagacag aggagaccac accatgcccg tgaagagccc
    39061 ccgccccggc gacctcgacc cgatcgagac cgccagccgc gacgaaattt ccgccctgca
    39121 gctgcagcgc ctgcagtggt cggtgcgcca cacctacgac aacgtcgcgc cctaccgcaa
    39181 gcgctgcgag gaagcgggcg tgcatcccga cgacctccag tcgctggccg acctcgcgaa
    39241 gttccccttc atgacgaaga cggacctgcg cgacaactac cccttcggcc tgttcgccgt
    39301 gccgcgcgac aaggtcgccc gcctgcacgc ctccagcggc accaccggca agtcggtcgt
    39361 cgtcggctac acgaagcgcg acctcgacaa ctgggccgac gtggtcgccc gctcgctgcg
    39421 cgcggccggc gtgcgtgccg gcgacatggt gcacaacgcc tacggctacg gcatgttcac
    39481 cggcggcctc ggcgcccact acggcatcga gcgtgccggc tgcaccgcgg tgccgatgtc
    39541 cggcggccag accgagaagc aggtgcagca gatcatggac ttccgccccg acgcgatcat
    39601 ggtcacgccg tcctactcgc tggtgatcgc cgaggagttc gagcgcctga acatcaagcc
    39661 cgaggacatc tcgctgaaga tcggcatctt cggcgccgag ccctggggcg aaggcatgcg
    39721 cagcgagatc gagcgcaagc tcggcatcga cgccatcgac atctacggcc tcaccgaggt
    39781 catgggcccg ggcgtggcca gcgagtgcat cgagaccaag gacggcccgg tgatctggga
    39841 agaccacttc tatcccgaga tcatcgatcc cgacaccggc gaagtgctgc ccgacggcga
    39901 ggaaggcgag ctcgtgttca cctcgctcac caaggaagcc ttcccggtca tccgctaccg
    39961 cacccgcgac ctcacccgcc tgctgccgcc gaccgcgcgc tcgttccgcc gcatcggcaa
    40021 gatcaccggg cgctcggacg acatgctgat cgtgcgcggc gtgaacgtgt tcccgaccca
    40081 gatcgaggag cagatcatgc gcgaccgccg cctgtcgggg aactaccaga tcgtgctcac
    40141 gcgcgacggc catctcgaca acgtcgagat ccgcgtcgag gtccagcgcg aactctccgg
    40201 caagctcagc cgcaccgaga ccgagaacat cgcacgcgag ctgcagcacc acatcaagac
    40261 catcatcggc atctccacca aggtgaacgt gatggagttc gaagccattc cgcgcacgct
    40321 gaccggcaag gcccgccgcg tcatcgacca gcgcccgaag caactgtaag gagctggcca
    40381 tgactcaagc attcatctgc gacgccatcc gcacccccat cggccgctac ggcggcaccc
    40441 tggcctccgt gcgtgccgac gacctcggcg ccgtgccgct caaggccctg atgacccgca
    40501 acccccaggt cgactggacc gcggtcgaag acatcatcta cggctgcgcc aaccaggccg
    40561 gcgaggacaa ccgcaacgtc gcgcgcatgt ccggcctgct cgccgggctg ccgatcgagg
    40621 tgcccggcac cacggtcaac cgcctgtgcg gctcgggcat ggacgccatc ggcctggccg
    40681 cgcgctcgat caagtcgggc gagaccgagc tgatgatcgc cggcggcgtc gagagcatgt
    40741 cgcgcgcgcc cttcgtgatg ggcaaggccg agtcggcctt ctcgcgcagc gccgcgatct
    40801 acgacaccac catcggctgg cgcttcatca acccgctgat gaagaagctg tacgagacgc
    40861 actcgatgcc gcagaccgcg gacaacgtcg ccgccgactt cgacatctcg cgcgccgatc
    40921 aggacgcctt cgcgctgcgc tcgcagcagc gctgggccgc cgcccacgcc gccggtcgct
    40981 tcaaggacga gctggtgccg gtggtgatcc cgcgcaagaa gggcgacccg atcgtcttcg
    41041 acaccgacga gcatccgcgc cccgaaacca cgctggagat gctggccaag ctcaagggcg
    41101 tcaatggccc cgagctcagc gtcaccgccg gcaacgcctc gggcgtcaat gacggcgcct
    41161 gcgcgctgct gctggcctcc gacgccgccg ccgcgaagca tggcctgacc ccgcgcgccc
    41221 gcgtcgtcgc catggccacc gccggcgtgg cgccgcgcat catgggcttc ggccccgcgc
    41281 ccgcggtgcg caaggtgctc gccaaggcgg gcctcacgct cgatcagatg gatgtgatcg
    41341 aactcaacga ggctttcgcg gcacaaggcc tcgccgtgct gcgcgacctc ggcctcgccg
    41401 acgacgaaga acgcgtgaac cccaatggcg gcgctatcgc cctgggccac ccgctgggca
    41461 tgagcggcgc ccgcctggtc accacggcgg cctacgagct gcagcgccgc aatggccgct
    41521 acgcgctgtg cacgatgtgc atcggcgtcg gccagggcat cgcgatgatc atcgagcgcg
    41581 tctgaggcac gggacagtcc atgaactacg aaaccctcgc catcgagcgc cgcggcgccg
    41641 tcgcgacgat ctggatggat cggccggcag tgttcaacgc cttcaacgag cagctcatcg
    41701 ccgagctggc ggccgcctgc gccgaactcg acgccgatgc cggcgtgcgc gtggtggtgc
    41761 tggccgggcg tggcaagcac ttctcggccg gtgccgacct caactggatg cgccgcgcct
    41821 cggacagcac cgaggcggag aacctcgccg actcgcgcaa gttcgccgcc atgctgcgca
    41881 cgctgtcggg gatgtcgaag ccgaccatcg cgcgcgtgca gggcgcggcg ctcggtggcg
    41941 gcaccggcct caccgccgcc tgcgacatgg cgatcgccgc ggacgatgcc agcttcgcga
    42001 cctccgaggt caagttcggc atcatccccg cggtgatcag cccctacgtg ctgcgcgcca
    42061 tcggcccgcg ccacgccctg cgctacttcc agagcgccga acgcttcggc gccgccgagg
    42121 ccaaggccat gggcctggtc ggcgaggtcg tcacgctgga tgaactcgac gccgcggtcg
    42181 atcgcctcgt gcaggccctg ctcgcctgcg gcccgcaggc ccagcttgcc gccaagcagc
    42241 tgatcgccgc gctggccggg cgtccgatcg acgacgccgt cgccgaggag accgccggcc
    42301 gcatcgcgcg ccagcgcgcc accgccgaag cccgcgaagg cttcacggcc ttcttcgaga
    42361 agcgctcgcc ggcgtggatg caatgaacac ccgccgcgcc ccgggcgtgg cggacctttg
    42421 aggaggaggc ggggtcacag ccatgcgcac cccgcgttcc gtgccggtat ttcccggtgc
    42481 ggtgtttgct aggaggaaac acatgtacac ccaggcaatg gacattccgg acgattccgg
    42541 aaagaaactc gcagcggtct ccaatgcgac gagcgagcag gaagccgcct tcgaggcccg
    42601 catcgacgcc gacgggcgca tcgagccgca ggactggatg cccgaggcct accgcaagac
    42661 gctggtgcgc cagatcagcc agcacgccca cagcgagatc gtcggcatgc tgccggaggg
    42721 caactggatc tcgcgcgcgc ccagcctcaa gcgcaaggcc atcctcatcg ccaaggtcca
    42781 ggacgagggc ggccacggcc tctacctgta ctccgctgcc gagacgctgg gcaccagccg
    42841 cgaccagatg ctcgaaggcc tgcactcggg caaggccaag tacagctcga tcttcaatta
    42901 cccgacgctc aactgggccg acgtcggcgt gatcggctgg ctggtcgatg gcgcggcgat
    42961 catgaaccag gtcccgctgt gccgctgctc ctacggcccc tacgcgcgcg ccatggtgcg
    43021 cgtgtgcaag gaggaatcct tccaccagcg ccagggctac gaggccctgc tggtgatgat
    43081 gaagggcacc gacgagcagc gcgcgatggt gcaggacgcg gtcaaccgct actggtggaa
    43141 gtgcctggcc atgttcgggc cgccggatgc cgacagcccg cacagcgcgc aaggcatgcg
    43201 ctggggcatc aagcgcatca gcaacgacga cctgcgccag aagttcatcg acgccaccgt
    43261 gccgcaggcc aaggtgctgg gcgtgaccct gcccgacccc gacctcaagt ggaacgaggc
    43321 gcgcggccac tacgactacg gccagatcga ctgggccgag ttctggaaca ccgtcgaagg
    43381 caacggaccg tgcaacaagg aacgcctggc cacccgggtc aaggcacaca acgatggcca
    43441 gtgggtccgc gacgcggccc aggcctatgc ccgcaagcaa aaagaacgcg cactgaagga
    43501 ggcagcatga acacccccgc actcaaggat tggcccctct gggaagtctt cgtccgcagc
    43561 aagcaaggcc tcgagcacaa gcactgcggc agcctgcacg cggccgacgc cgcccaggcc
    43621 ctgcacatgg cgcgcgacgt gtacacccgc cgccaggaag gcgtgagcat ctgggtggtg
    43681 ccctcggtcg ccatcaccgc cagcaacccg gacgacaagg gcgagctctt cgaccccgcg
    43741 gccgacaaga tctaccgcca tccgaccttc tacgagatcc ctgaagaagt ggggcacatg
    43801 taatgagcac gccgatcgac tacctgctgc acctggccga caacgccgtc gtgctcggcc
    43861 agcgcaacgc cgaatggtgc gggcacggcc ccatcctgga agaagacatg gcgctggcca
    43921 acgtcagcct cgacctcatc ggccaggcgc gcctgctgta ccagcgcgcc gccgagctga
    43981 agggcggcga cgccaccgag gacaagctgg cctatttccg cggcccgcac gagttccgca
    44041 actacacgct gctggagctc ccgcaccacg gcgcgctggt cggctacgcc gcggccgacc
    44101 gcgactacgc caccacgatc gtgcgcaact tcctgtattc ggcgctgatg gtgcacgtgt
    44161 gggacggcct gcagcagtcc gccgacgcca gcctcgccgc gatcgccgcc aagtcgctga
    44221 aggaaaccag ctaccacctg caccactcgc gtgactggct ggtgcgcatg ggcgacggca
    44281 ccgaggagtc gcacgcccgc gcgcaggcgg cggtgaacca cctcatgccc tacaccgagg
    44341 agttctggac caccagcccg gccgaaggcg ccgcggtggc ggccaagatc ggcatcgacg
    44401 tatgggcgct gcgcccggcc tgggacgcgc tcgtcaacga ggcgctggcc gaagccaccc
    44461 tgacccgccc gaacttccac ggttacgtga ccgagggcaa gatcggcctg cactccgagc
    44521 atctgagcta cctgctcggc gagatgcagg gtctggcccg cgcccacccg ggcgccacct
    44581 ggtaagggcg aaggccatga gcagcgtcgt ggacaaggtc tgggccaccc tggagcccct
    44641 gacggacccg gagatcccgg tggtgaccct gcgcgagctg ggcatcctgc gggatgtgcg
    44701 ggagacggcg gagggcatcg aggtggtcat caccccgacc tacagcggct gccccgccat
    44761 ggggcagatc gaggacgacg tgcgtgcgag cctggaacag gccggcatcg cggcgcgggt
    44821 gatcacccag ctcgcgccgg cctggaccac cgactggatg agcgacgagg ccaaggaaaa
    44881 gctgcgcgcc tacggcatcg ccccgcccca ccgcacctgc ggcagcagcg gcggcgccga
    44941 ggtcagcgtg ctgaagttcg tgcgccggca gccgaccgcg gcggtcgcct gtccgcagtg
    45001 cggctccacc cacaccgtcg tcacctccga gttcggctcc acggcctgca aggcgctgta
    45061 caagtgcctc gactgccagg agcccttcga ctatttcaaa ccctattaag agccggagcc
    45121 cagacgccat gtcagccccc cgtttccaag aactcgccat caagcgcgtg agccccgagg
    45181 ccgccggcgc ggtggcgatc accttcgcca tccccgaggc cgagcgcgag cgcttcgcct
    45241 tcgagccggg ccagttcctc accctgcgcg ccaccatcga tggccaggac gtgcgccgca
    45301 actactcgat cagcagcccg cgcagccgcc tggcacgcga gggcgagctc gagatcggca
    45361 tccgcccggt ggaaggcggc ctgttctcga actgggccac gcgcgccatc aaggccggcg
    45421 acaccctgca ggtgatgccg cccgacggcc gcttcgtggt caagaagaag cgcgccatcc
    45481 accgcgtcgg cttcgccgcc ggctcgggca tcaccccgat cctgtcgatc gccgcgagca
    45541 cgctggaaga acagccggac tcgaagttca cgctgatcta cggcaaccgt cgcatgtcca
    45601 cggtgatgtt caacgaggac ctgcaggacc tcaaggaccg ctaccgcgac cgcctgacca
    45661 tgatccacgt gctgtcgcgc caggcccagg aagtggacct gctgcagggg cgcatcgacg
    45721 gtcccaaggt gcgcgcgatc atcgacgccc tgctgccggt gggcagcatg gacgaggtct
    45781 tcatctgcgg ccccgacgag atgatcaccg ccaccgagac cgcgctggtc gaggccggcg
    45841 tgcccgccga ccgtatccgc accgagcgct tcaccgccaa cctgcccgcc ggcgcccacc
    45901 cggtcggcgc ctcgagcacc gccgaggccg tcgcggccgc gaccaaggac atcaccatgg
    45961 tcctggtgct cgacggcaag gagcacgaga tcgcgatcgg ccccgacgag cacctgctcg
    46021 acgccggcct caatgccggc ctcgacctcc ccttctcgtg caaggccggc gtgtgctgca
    46081 cctgccgcgc caaggtgacg gagggcgaag tcgtgatgga caagaacttc accctcgagg
    46141 ccgacgaagt cgcgcagggc tacgtgctga gctgccaggc gcgcgccacc accaagcgcc
    46201 tcaagatcag cttcgacgag cgctgacacg atctgcgggc cgacggcgtc cgcgcgcgca
    46261 gcagcttccg acggccgtct cgacggccgt tttccttcgc ggacggagac gcggccgcgc
    46321 gcccctgacg caggtcaacg ccgaaagggc agcggcctgc tatcccttta gacctcatcc
    46381 actttccagc gcgaagatca tggccggctc ccacgctctc agcaccgcct tcgaaccgtc
    46441 ctcgatcgcc gtcatcggcg cgtccgacgc acccgggtcc gtcggccgcg tcatcttccg
    46501 caacctggtc gaaggcggct atcgcggcac cctgtacgcg gtcaacccga agtacggcag
    46561 catccaggac catccctgcc accgctcggt cgaggacatc ggccgaccgg tcgatctcgc
    46621 catcatcgcc acccccgcgc gcatgctgca cagcgtcacc gcacagtgcg ggcgcagcgg
    46681 cgtacgcaac gcggtcatca tcagcgccag cgggaacgca ctcgagcgcc gcatcgtcga
    46741 gaccgcgcgc gagacccggc tgcgcctgat cgggcccggc agcctgggca tcgcacgccc
    46801 cggcagcggg atcaatgccg cgctcacccg catcgccgtc aagcccggcg agatcgccct
    46861 gatctcgcag tcgggcgcga tgtgctcggt cgtgctcgac tgggcgttga ccaacgacgt
    46921 cggcttctct tcggtcatct cgctcggcgg cacggtggac atcgactacg gggaaacgct
    46981 ggagtacctg atccacgaca cgcagacgcg ctacatcctg ctgcacgtcg atcacatccg
    47041 caacgcccgc cagttcatga gcgcactgcg ctcggccgcg cgcgtcaagc cgatcatcct
    47101 gctcaaggcc ggccgccacg gtcgcagcga caacaacgcc gccgacgcgg acaacgccgg
    47161 cctcgccgac gtggtcttcg acgcggcgat gcgccgcgcc ggcgtggtgc gggtgcagaa
    47221 catcgaccag ctcttcttcg ccgccaaggc cttgtcctgc ggctttcgac cgcgcgagga
    47281 cagcctcgcc atcgtctcca acggcagcgg cccggccgat ctcgccgccg accgcgccaa
    47341 ggacctcggc accggactcg tcgggctggg ctcgccgacg gtcgccacgc tcgagcgcct
    47401 cggcggacgc gagcggcact cccacaaccc gctcgacctc ggcggcgacg ccagcgcgca
    47461 gctgtaccgc gacgccatcc tcgccctctc cgaggacgcc gcggtttcca acgtgctggt
    47521 gatcctgtcg ccgcacgcgc tcgtcgatcc ggtcgaggtg gcgcaggaca tcatcgacct
    47581 cgccgcccag gtgcggctcc agctgtgctg ctgctggatg ggcggcggcc aggtcgccga
    47641 ggcgcgcgcg ctgctcgccc gggccgggct gccggtgttc agttcgcccg aggcggcgat
    47701 cgagctgttc cacaacatct cgcgcttcca ccacaaccag accctgctgc tgcagaccgc
    47761 cggcggcggc agcgccgccg cctaccgccg cacgggtggt gcgcgcgtgc tgatcgagac
    47821 cctgctcaac cagcgtcgcc atatcctctc ggcgatggag tccaaggcgc tgctgcgcag
    47881 cttcggcatc ccggtcacgc acaccaccgt cgcccgcgac gccaccgagg ccttgttcgt
    47941 cgccgagcag gcgggttttc cggtggcgat gaaggtcgac gctcccgagc tggcgagcaa
    48001 gtccgaatcc ggcggcgtgc gcctcaacct caacaccgcc gagtcggtgt ggagcgcctt
    48061 ccacgacatc gtcgacgcgg tgcgcctgcg ccaccccgag gtgcagatca acggcgtgtc
    48121 gatcgagccc tatctgtacc ggccgcaggc gcgcgccttc cgcatccagg tgcatcgcga
    48181 cgcgatcttc ggtccggtga tcagcctcgg cgcgcaggac gcctccgcgc cgcgcacctt
    48241 cgcgctgcca ccgctgaacc ccgtgctggc ccgcgacctc atcgactcgg ccatcccggc
    48301 ggccgcacag ggcccgcgca gccagggcgg cgcgctcaac cgcgccgcca tcgagcaggt
    48361 gctggtgtcg gtgtccgacc tcgtgtgcga gctgccctgg gtgcgcgagc tcgcgatcga
    48421 ccccttgttc atcgacggcg acggcgccat cgcggccgat gcccgcgtcg tcatcgacca
    48481 gggccttccc gccggcagcg accgctacgc gcacatggcg atccacccct atccgctgca
    48541 cctcgtccag gactggaagg tcggcgacgg tcgtgccgta cgtacgcgtc cggtgcgccc
    48601 ggaggatgcc aacctggtgc tgggcttctt cgacggactg agcgccgaga cccgcttcat
    48661 gcgcttcatg gaagagctgc aggagctcag cccggcgctg gtcgcgcgct tcacgcagat
    48721 cgactacgac cgcgagatgg cgctgatcgc caccaccatc gacgacgccg gcgccgagca
    48781 catggtgggc agcgcccgct attccctcgc cgccgacggc gaatcggtcg agttcgccct
    48841 ggtcgtggcc gacgactggc aacgccacgg actgggccgc cgcctcatgg gtgccctcat
    48901 cgaggtcgcg cgcggcaagg gctatcgcaa catcttcggc gacgtgctgg gcaagaacgc
    48961 caagatgctg cgcctgatgc acggcctcgg cttcctcgtg cagccccacc cggaagagag
    49021 cgcgttgcgc cgggtggtga aggtgctgca tggcaagtag cgcgtccgca gcggcgcaga
    49081 cggccgctca ataatcgccc cagcgcgcct gcagcgccgc cacggcggcg aggcccgcgg
    49141 tctcggtgcg cagcacgcgc ggcccgagcc gcacgcgccg gcaaccggcg gcgtcgaagg
    49201 ccgccatctc gccctcgctc cagccaccct ccggcccgac cagcaggtgc acctcgtcct
    49261 tcggctcggg ttcgtcggca aagcccttgg ccccgccggg cgcaagcacg tagccgggcg
    49321 cagcgcgatg cgcgcccagc cagtgtgcga gcggctgcag ggggcggatc tcggggacgc
    49381 ggttgcgccc gcactgctcg caggccgcga tcgcgacctg ctgccagtgc gcgacgcgct
    49441 tgtcggcacg ctcggccgcc agacgcagca ccgagcgctc ggcctgcacc ggctggatcg
    49501 catgcacgcc gagctccacc gccttctgca ccacccagtc catcttgtcg cccgaggcca
    49561 gcgcctgcac cagcacgatg cgcaacggcg actcgcgttc gaccgcacgc gcctcgccga
    49621 gccgagcgaa caccgccttg ccgcgcacgg ccaggcgcgc atcgacctct ccgccgctgc
    49681 cgtcgaacag caccaccgcc tcgccatcgc gcaggcgcaa cacccgcagc gcatggtggg
    49741 cgagcgcctc ggggagcgcc acctcgccgc ccgcgggcag gcgatccgga aagtggaatc
    49801 gtgaaatcat cgtaagatta taggcccagc tcccctcgcc ccctgcccct ctcccgccgc
    49861 ccgccggcaa ggagcccgcc atgcccaccg ccgcccaccg cctcgcccgt gcccgcgccc
    49921 tcgaatcgat ggtgcgcgac atcgcgcgcg ccgagatcct tccgcgctac ctgaagtccg
    49981 cgcgcaaccg caaggccgac ggttcgctgt tcaccgaggc cgacatggcc tcgcagcagc
    50041 gcttcagcga ggccctgccc gccttcctgc ccggcgccgt gctcggcgag gagatgaccg
    50101 cggccgagca ggccgcgctg tggaaggaag gcgcgcgcgg cctgtggtgc atcgacccga
    50161 tcgacggcac caccaacttc gccaacggca tccccctgtt cgcggtgtcg atcgcctatc
    50221 tgatcgacca cgagccggtg atcggcgtgg tgtacaaccc gatcaccgac gaatccttct
    50281 acgccgcccg cggcgccggc gccttcctca acggcaccga gctgccgctg cgcgcaggcg
    50341 ccaccgagtt gcgcgacgcc gtcgccgggg tggacttcaa gcgcatcagc caccacctcg
    50401 gcgacgagct cgcggtgcgt ccgccctact actcgcagcg caacttcggc tccagcgccc
    50461 tcgaatggtg ctttgtcgcc gccggccggc tcgacgtcta cctgcacggc gggcagatgc
    50521 tgtgggacta cgccgccggc cgcctgatcc tcgccgaagc cggcggcatc gccgaggcgc
    50581 tcgacggcgg cgtgctgatg tccggcccgg cgatcaagcg cggcgtcatc gccgccacca
    50641 gcccggcgct gttcgccgaa tggtcggcct gggtcaaggc gcgctcctga tggctcgcac
    50701 cgcgctttat tgcggtgcag aatccgtgtc gaaaccgact cgccagcggc ggaacccatc
    50761 atgtccatga agcacccgat catcgccgtc accggctcgt ccggcgccgg caccaccacg
    50821 gtcaagcaca ccttcgaagc gatcttccac cgcgagaacg tcaacgccgc catcgtcgag
    50881 ggcgacagct tccaccgcca cacccgcgcc gagatgaaga cgctgatcga ggaagccgag
    50941 cgtgcgggcc tgcgcgggat cagccacttc ggccccgagg ccaacctgct ctccgagctc
    51001 gagagcctct tccgcgccta cggcgaggcc gccaccggcc gccgccgcta ctacatccac
    51061 aacgaagccg aggcgatgcg ctggaacctg ccgctcggca ccttcaccga gtgggaagat
    51121 ctcccggtcg gcaccgactg cctgttctac gagggcctgc acggcgcact cgtcaccgac
    51181 gaggccgaca tcgccagcca cgtcgacctg ctgatcggcg tcgtgcccat catcaacctc
    51241 gagtggatcc agaagctgca ccgcgacacc aagctgcgcg gctacaccgc cgaggccgtg
    51301 caggacacca tcctgcgccg catgcacgac tatgtgcact acatcgtccc gcagttcgcc
    51361 cgcacccaca tcaacttcca gcgcgtgccg gtggtcgaca cctccaaccc cttcatcgcg
    51421 cgcgacgtgc ccacccagga cgagtccatg gtggtgatcc gcttcaagga cccgcgcgga
    51481 gtcgacttcc cctacctgct gcgcatgatc cacgacgcct tcatgagccg cgccaacacc
    51541 atcgtgatcc ccggcggcaa gctcgacctc gccatgcagc tgatcctcac cccgctcatc
    51601 tggaagctga tggaacgtcg ccgccagtgg gtttgaggcg cgacggaatt cctcggcagc
    51661 acaccgacca gccccccagc cccactccgc gcgaggtcgc ggggtcgggg tggtgtgcgc
    51721 caacttcagg agcgccgagg tggagggcgt cgcgggggaa acaaggcgac g
//
LOCUS       DAOQBS010000008        50753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_304_length_50753_cov_12.200501, whole genome shotgun sequence.
ACCESSION   DAOQBS010000008 DAOQBS010000000
VERSION     DAOQBS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 50753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50753
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_304_length_50753_cov_12.200501"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..63)
                     /locus_tag="PKN25_01925"
     CDS             complement(<1..63)
                     /locus_tag="PKN25_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309313.1"
                     /note="with FlhD is involved in the activation of class 2
                     flagellar genes and as well as a number of other genetic
                     systems; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HNV89683.1"
                     /translation="MKNKSVIEEADDVRRAVEMVQ"
     gene            complement(74..391)
                     /gene="flhD"
                     /locus_tag="PKN25_01930"
     CDS             complement(74..391)
                     /gene="flhD"
                     /locus_tag="PKN25_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HNV89684.1"
                     /translation="MDRPDFQEEIRELNLAYLMLAQQMLRDDRSTALFRLGIGEAFAD
                     LIESMSAAKLVRMASSPTLLPCFRFDDAQLARLMAGEGRDAASAGLHAAILAAGKAAA
                     DAR"
     gene            complement(548..2476)
                     /locus_tag="PKN25_01935"
     CDS             complement(548..2476)
                     /locus_tag="PKN25_01935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV89685.1"
                     /translation="MPQPIDMKRYAQLKAAGDLPSPRGVALAIIRLTQAPDVSLAELG
                     RVIKGDPAFVGRLIKAANGLIAENRRAIVSVQEALMVLGLPAVRTLALGFSLLSTYRK
                     GTCQGFDYPRFWSSALLMAISMQALTQRVRVVAPDEAFSLGLLARVGELSLATLYPEA
                     FSRLLAELKRDPALQQVVLEDQAFAMNHRELGAAMLADWGVPDALVQPVRFHEQPELA
                     GYAEDSREAGIVQCLVLARAVAQLCLAPDSEHVRLMAPMLRLAGRLGLGRGEFVSLCE
                     RVGRDWAEWGKLLQIRTEDAPRFAEVGSAAVDAVGEAPKVAAEAAGPQPMRALVVAGA
                     REDRELLRGELEAAGIVPFTAAGADAALEAALDVQPQLLVADWSEEGERVVRGLRDMR
                     LGRGVYVICMLGSDDEARLLAASGAGVDDFLVRPLRRAALTVRLRACVRTLVLQQELE
                     HEREELRNFAAELSISNRRLQEAALTDPLTGLPNRRRAIEAMEAEWATATRHGRAFSV
                     MIVDLDGFKTINDTHGHDVGDMALRQAAATLRGALRAQDLICRTGGDEFLVLCPDTDL
                     KAAMLVAERLRVAVQASPVETGGPQIFLTASVGVAMRVPAMGSVDALIKCADDSVYRA
                     KQRGRNCIAALQLQSGAG"
     gene            2621..3169
                     /locus_tag="PKN25_01940"
     CDS             2621..3169
                     /locus_tag="PKN25_01940"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNV89686.1"
                     /translation="MKYCSNCGATVAFRIPPGDTLPRHVCEQCGTIHYQNPKLVVGAL
                     AEWEDRVLLCRRAIEPRHGMWTLPAGFMENEETTAQAAARETLEEACARIEVGELYTL
                     IDVPHISQVHIIYRARLLDLDFRPGEESLEVALLREDEIPWERIAFRSIAISLRHFFD
                     DRRSGRWQLHTASLAPPPPDWR"
     gene            3266..3850
                     /gene="rsxA"
                     /locus_tag="PKN25_01945"
     CDS             3266..3850
                     /gene="rsxA"
                     /locus_tag="PKN25_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNV89687.1"
                     /translation="MTSYIFVVIGAVLVNNVVLVRILGLCPFMGVSKKLETAIGMGAA
                     TTFVLTVGAGTSYLIDHYLLQPFDLGYLRTLAFIVVIAAIVQLTELIIQKTSPLLHQV
                     LGIYLPLITTNCAVLGVPLLNVGLKHDLLESLLFGFGSSLGFTLALVLFAGIRERLDG
                     ADVPIAFKGTAIAMITAGFMSLAFMGFAGLDRFH"
     gene            3864..4415
                     /gene="rsxB"
                     /locus_tag="PKN25_01950"
     CDS             3864..4415
                     /gene="rsxB"
                     /locus_tag="PKN25_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HNV89688.1"
                     /translation="MITAILVMAGIAIVLGLTLGYAAIRFKVEGDPLVEKIDAILPQT
                     QCGQCGFPGCKPYAEAIANGEADINQCPPGGEEGVRKLADLLGREFKPLSEEHGVEKP
                     KSVAVIDEQVCIGCTLCIQACPVDAIVGAAKQMHTVVEPLCTGCELCVAPCPVDCIAM
                     VPLAETVHTWKWKYPVVEMRRVA"
     gene            4415..6136
                     /gene="rsxC"
                     /locus_tag="PKN25_01955"
     CDS             4415..6136
                     /gene="rsxC"
                     /locus_tag="PKN25_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNV89689.1"
                     /translation="MLRELFRFHGGVKPATNKSASTTSPIAKAPIPARLVVPLHQSLG
                     SPAIALVEPGQRVLKGERIGAAGGRLSAAIHAPTSGVVRAVEPALVPHASGLREPAVI
                     IESDGEDRWAEPEHINYRITPPDEVRDFVRDAGVVGLGGAVFPTHQKMRPGSGGKTRT
                     LVLNGAECEPFITCDDMLMRERADGIVSGAAILRHMLQAEEVLIGIEDNKPEAIAAMR
                     QAVAAAGEHMEVVAVPTRYPAGGAKQLIRVLTGIEVPHGERSTDYGVQCFNVGTAYSV
                     HEALELGRPLVSRIVTVAGNVERPGNYEVLIGTPMDELVRLCGPRADTRFLVMGGPMM
                     GVRMPAFDVPVVKATNCVLAADDKLFPPAPPEQPCIRCTECAKACPVELQPFELYWFS
                     RSKNFGKAQEYHLFDCIECGCCAYVCPSHIPLVDYYRFSKGEIWAREREKAAADQARE
                     RFEFRNFRQEREKAEKAAKLAAKAAETRAKLAEAGTAEAPAAAEAPAAEDPKKALIAA
                     ALARAKAQKAEIQPQNTDNLSPQTQAEIADIEARRKALEAAAVPASTAPAPQAADATD
                     LPDEPRP"
     gene            6144..7160
                     /locus_tag="PKN25_01960"
     CDS             6144..7160
                     /locus_tag="PKN25_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNV89690.1"
                     /translation="MIHSPYVRKAVSVQRTMGLVLLALIPGIVAHALLIGPGVLVNLA
                     ISTITAVGAEALMLHLRKRPVAIAVGDLSAVVTAWLVTLCFPPIVPWWLTVCGALVAI
                     VAVKHLYGGLGQNPFNPAMIAYCSMIVAFPALMSQWPPAGQLSFDTQLALILGGARDI
                     DGITGATALDALRTGLRSGTQTVDAVLQGSAFGALGGRGWEWISVGYVAGGLFLLAKR
                     IITWHMPLAFIGVLAGISTLFWLLDPGRFASPLFHLASGGAMLAAFFIVTDPVSGATT
                     PRGKLLFAGGIALIAWLIRNFGAYPEGIAFGVLLMNMCVPLLDMKTQPPVFGHHKDAK
                     GGGK"
     gene            7157..7825
                     /locus_tag="PKN25_01965"
     CDS             7157..7825
                     /locus_tag="PKN25_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNV89691.1"
                     /translation="MSARYSATRTALRTGAIMVVFTLVFTALMAATHRVTQPAIEASA
                     QDAQRRLIDEVLPPGSYDNDLLADVVRFGPAPQIGMDEGGRVWRARKGGEPVALIAEG
                     AATDGYSGRIALLVAVNTNGLLSGVRVTQHKETPGLGDYIDPKKDRRKDMPWIGQFAI
                     ATWKDVNTAAWTVRKDGGTFGYRTGATISARAVVRAAGRSTAFVVERMDALFAAPSGG
                     VLGE"
     gene            7828..8547
                     /locus_tag="PKN25_01970"
     CDS             7828..8547
                     /locus_tag="PKN25_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNV89692.1"
                     /translation="MSPQQFREIAWNGLWKQNTGLAQLLGLCPILAISTSMVNAVSLG
                     LATILVMAMSNLAVSALRNFIPYEIRIPVFVLIIAALVTVVDLSFNAFFHDLYLVLGI
                     FIPLIVTNCIVLARVEAYAAKNDPLTSTVDGIMMGIGLVWVLAVLGGMRELLGAGTLF
                     SGIEMILPGASAWNVFGDGYPGFLIAILPPGAFFALGVLIAAFNAINVRLAARARRNP
                     PPAPTQTAEPKEDAAEPAAAG"
     gene            8565..9197
                     /gene="nth"
                     /locus_tag="PKN25_01975"
     CDS             8565..9197
                     /gene="nth"
                     /locus_tag="PKN25_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNV89693.1"
                     /translation="MKREAIREFFRRLHEADPHPQTELEYASPYQLLVAVVLSAQATD
                     RGVNLATRKLFALAPTPEAMVSLGEEGIAECIKSIGLFRNKAKNTLALSRLLLERHGG
                     EVPAVREALEALPGVGRKTANVVLNTVFRQPAMAVDTHIFRLANRTGLAPGKDVLEVE
                     KALLRRVPKDYLLDAHHWLILHGRYVCTARKPKCGECGVRDLCLCRDKTD"
     gene            9295..9729
                     /locus_tag="PKN25_01980"
     CDS             9295..9729
                     /locus_tag="PKN25_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HNV89694.1"
                     /translation="MFNPSRDQVRSFFIDTWRKARAKEVLTPMETIAADIIALHPEYH
                     AVVEDPEAVHRDFPPEAGQINPFLHLSLHLAIEEQLSIDQPPGIRAAFEAACQHRGDR
                     HDAMHDALECLGEMLFDAQRKGTPPDGAAYVGALRKRAGLAL"
     gene            9926..11053
                     /locus_tag="PKN25_01985"
     CDS             9926..11053
                     /locus_tag="PKN25_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNV89695.1"
                     /translation="MPGLLTHVDPDGLLEYSVVYTDRSLNHMSQAFQGVMRELSATLK
                     KVYHAHAVAIVPGSGTFGMEAVARQFATGRKTLVIRNGWFSYRWTQIFEMGNIPAESV
                     VLKARQVRDEAQAPFVPAPIDEVVAAIRQHRPDVVFAPHVETSAGMMLPDDYLRAVAD
                     ATHAVGGLFVLDCIASGTIWVDMDATGVDVLVSAPQKGWSASPCCAMVMLSAAARERI
                     EGTTSTSFACDLKKWLQIMEAYEGGAHAYHATMPTDGLVTLRNVMRETEAYGFDKVKA
                     EQVELGAGVRALLEARGFPSVAAAGFQAPGVVVSFTTDDGIKTGAKFAAAGLQVAAGV
                     PLACDERPDYKSFRVGLFGLDKLHNVARTVATLEKALDQVG"
     gene            complement(11170..12513)
                     /gene="hflX"
                     /locus_tag="PKN25_01990"
     CDS             complement(11170..12513)
                     /gene="hflX"
                     /locus_tag="PKN25_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNV89696.1"
                     /translation="MQTDLADEPRKAIVASVQRPNVSDAEFEASLAELRELAKTLGYR
                     VVHTFTQKRAGFDTTGYLGVGKRQEVREFVAAQAGFDAAPTDTVPRTGAIEALLVDHE
                     ISPSQARNLELETGCEVMDRTMVILEIFHRNARSRAAKAQVEIARLGYMAPRLREAAK
                     LAGPQGRQRSGVGGRGAGESHTELDRRKIRDRIAELQLEIVAMEAERKTQRARRQGRQ
                     SPASVALVGYTNAGKSTLMRALTGSEVLVANKLFATLDTTVRTLYPESVPRVLVSDTV
                     GFIKNLPHGLVASFKSTLDEALDAGLLLHVIDASDPGFERQLEVTDSVLQEIGADELP
                     RIRVFNKIDHVGDAAAQAECEAALRAKYPGCIVMSARRPDEVARLRQTIVAFFQQDLV
                     EAELLLPWSAQQLRKEIYANCEVLEERAEDEGAVFRLRGEPDTVERLRAQCALTR"
     gene            complement(12604..13002)
                     /gene="cueR"
                     /locus_tag="PKN25_01995"
     CDS             complement(12604..13002)
                     /gene="cueR"
                     /locus_tag="PKN25_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HNV89697.1"
                     /translation="MNIGKAAAASGVSAKMIRYYESIGLLPAARRTDAGYRFFTGEDV
                     HVLRFIRRARDLGFSLADIGDLLALWRDQGRASADVKRIALGHVAALERKIAELQGMV
                     DTLRTLAAHCHGDARPDCPILTDLDAGGGR"
     gene            complement(13259..15784)
                     /locus_tag="PKN25_02000"
     CDS             complement(13259..15784)
                     /locus_tag="PKN25_02000"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNV89698.1"
                     /translation="MNTPRTVSPAAAVRTLAIAGMNCASCVAHVEKALLKVEGVQAAS
                     VNLATETATVTAPAAVPLSALIAAVDKAGYAVRRDTLELDIEGMSCAACVGRVEKVLQ
                     RVPGVVTANVNLAARRAHVELAGGVPTAALVEAVQKAGFGARPAAEVGEGVREADAAA
                     EEAALRRAVWIALALTLPVFVLEMGAHLIPGMHDWVMATIGHRESWLLQFVLTSAVMF
                     GPGLRFFRKGVPALLRGAPDMNALVAVGTSAAWGYSVVALFAPALLPAGTVNVYFEAA
                     AVIITLILLGRLLEARARGRTSDAIRRLMRIQPRTARVRRDAGVVELPIAEVAVGDVV
                     EVRPGEKVAVDGEVVEGASFVDESMITGEPVPVEKGAGATVVGGTLNTRGAFAFRATR
                     VGADTVLAQIIRMVEAAQGAKLPIQALVDKVTMVFVPVVMGVAALTFLAWLLFGAAWL
                     PADVPVLTFALVNAVAVLIVACPCAMGLATPTSIMVATGRAAQMGVLFRKGDALQLLR
                     DAAVVALDKTGTLTVGKPQLTDLVVAEGFARADVLACVAAVERASEHPIAEAIVVAAR
                     AEGLDIAAAEGFGAEPGYGVRARVGGQRVEVGADRLMRRLGLEPDVFAATAARLADEG
                     KSPLYAAIDGRLAAILAVADPLKPSSVAAVRALHDMGLKVAMITGDNARTARAIARQA
                     GIDEVVAEVLPDGKVAALRRLAGRAGDGEAAGRRVAFVGDGINDAPALAEADVGIAIG
                     TGTDVAIESADVVLMSGDLTGVPRAIALSRATMRNIGQNLFWAFAYNSALIPLAAGVF
                     YPAFGALLSPVFAAAAMSMSSVFVLGNALRLRRFGADAPQTAG"
     gene            16077..17540
                     /locus_tag="PKN25_02005"
     CDS             16077..17540
                     /locus_tag="PKN25_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNV89699.1"
                     /translation="MSTSNSIRSGIKWLVTGSLGGQVLQFAFGIVLARLLVPEDFGFI
                     VTIQIFTGFVGLISGGGMGQALVQSKDAQARDFDVVFTAQLLIGLLIFAGFYVAAPWF
                     AHTFGDALYADLVRVSAISFLLRPFVNTRASALHRDMRFKERSVIGLAVAVLTGLASI
                     ALAVVGAGPWSLILSGILGSLITLAMLDRVSDRRPQLCLDLEVARRFGAYGVKVSLND
                     LAAYFTKQASNAIISRVAGPSAVGLFNKGESLAWLPFSTVSAAVYDAVFRAMAKAQDR
                     PDEVKYLFYRMIGLMVVYTLPIYIGLAWMAEPFMLFVYGAKWVPSADPLRIVSLAGLL
                     ICIGHPCGAVLAAMNRLGREVWIHIAQGVLVSVACYYGLKHSGISGAAWGVFAGIAFS
                     TPVMYYLATRCFPSRLGDLWRALAPGLGLNLILLAVLALADAALPADWREIRPALYML
                     VTGGCAAIAYAAAFLFLPLATLADESLRWRRTLRLAR"
     gene            17547..19532
                     /locus_tag="PKN25_02010"
     CDS             17547..19532
                     /locus_tag="PKN25_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89700.1"
                     /translation="MKHLDLLEHFSAADGGIAMQLRGAQRRLGSHDGVDIVRDDFADE
                     AASALFLRVQRTATAESVRLRLAGNHLLKRCAIGGWMFEPTTPGEAVFWVPRLPVCRT
                     LDGVGRIRAESPATLANMEIGGGEVAATFELAPDQVLDCVVWRLESEASGTDGSRAAD
                     ELMTASALESQAFFAYASHTATRCAADFYTHIVHGQVYGACWAWPKKLKICDELDALA
                     LHMVASALGHSTGKRVYRLLRRQIVLAVLCRQDADGGFRHGEWTDEYESHNRLINGAM
                     QLLATEFAAAPEPALEGALRRIARYLAEQVDHTDAGAWFLHDSLERSEEGMRHYPLAW
                     ERGRWLGKSPTNLLILNTHLDCMLALARERAVCGDDTHDGLLRSAEACLRTVMSARPA
                     DWLFRPLLAVIALSLLPKAEQEQLPFARRALKRLGWKYLIPRWHLIRRRFPRLLMPAG
                     YIERSLGQADFVHRYHGVHLMDFERLLACGAVDPKDPRWTSISDGLVDFGVNSRVTRL
                     WKETAASRDSLAFWAEGLYRRCLRTRAQRDRELLATAVLDLHDAGLGLPPSLLGANGE
                     IVPAQSAAPTPSAADARLRVINLGARKTPCLLVVNTATTDLPLAFEPPLTAAHPGWMD
                     ATRSVPARGWILLQPGPSDAEPGPASSSHAFLPARPR"
     gene            19535..20611
                     /locus_tag="PKN25_02015"
     CDS             19535..20611
                     /locus_tag="PKN25_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNV89701.1"
                     /translation="MSNARAIAFHLPQYHPTPENDEWWGKGFTEWTNTTRAKPLYPGH
                     YQPHLPADLGYYDLRLPEARHAQAELARAYGIEGFCYYHYWFGGRRILERPVNEILAS
                     GEPDLPFCLCWANHSWSNIWQGVADRMLIEQTYPGMDDHRRHFEWLLAAFHDPRYIRV
                     DGKPMFLIYNPPDIPDVARVMDYWRELAAQAGLPGLHLVAVNYLGAAVDPADFGMDAA
                     TWQPLPPKSGHIPWRYPALKARMRMAKGKYKLTVLDYARIMSGLTRASPPQFTEYPTV
                     LPNWDNTPRSGLNGLVLHGSTPELFKTVLRRGVDLVQGYPAEQRIVFIKAWNEWAEGN
                     YLEPDQRFGHGYLRAVREVLQETR"
     gene            20613..22469
                     /gene="asnB"
                     /locus_tag="PKN25_02020"
     CDS             20613..22469
                     /gene="asnB"
                     /locus_tag="PKN25_02020"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNV89702.1"
                     /translation="MCGINGIFAYAPAANAVDRAELLRVRDTMIERGPDGAGVWTGDD
                     GRIGLGHRRLAILDLSAAGAQPMHSADGTLVVSFNGEIYNYPELRGDLERAGVEFRSH
                     SDTEVLLHLYRRHGPAMVGRLRGMFAFALWDARARSLLLARDPHGIKPLYYADDGRTL
                     RFASQVSALLAGEGVDTRPEPAGAVGFLLWGSVPEPFTLYRGIRLLPAGSTLLVNEAA
                     GAGAAVRYWSLSAALQRSVQAAAAVPAGGEAAFMRERLLESVRAHLLSDVPVGAFLSA
                     GLDSSTLVGLAREASPARLRTLTLTFDDLQGTAHDECPAASLIARHLDVDHTCIVLSA
                     GEQEDELDRFFAAMDQPTIDGINTWFVSRAAQQAGLKVALSGLGGDELLGGYATFAEH
                     PRLRARAHASPWRHAPRLYRGAHRLLGARLGWPPNAAGIPACGADPEGAYHLLKGVFM
                     PWELDGVVDADVLCEGLQALAEHDAAHTEALAGLNDFGQVACLESVRYMRNQLLRDTD
                     WVSMSHSLEIRVPLVDHLLSEALLGLAASGRLGPGKSMLPRTLARGLPDEILNRPKSG
                     FVVPTWRWLRHHPGLDAWKSLPALRHPRMSDGRRWAYTLLSRHPAGKALIRN"
     gene            22508..23479
                     /locus_tag="PKN25_02025"
     CDS             22508..23479
                     /locus_tag="PKN25_02025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV89703.1"
                     /translation="METKPDPIPPATSARISVVMPCYNAAPFVREAVECVMGQTRGDV
                     ELIVVDDGSTDASRDLLHALAAAHGPRMRVLHQDRKGPYPARNLGLRHASGGLVAFLD
                     ADDYLSPDCLDKLANALERTDADIAYCGWQNVGEGAPGTTPYVPPDYAALDTAAEFLR
                     ACPWPIHAALVRRAAIDAVGGFSERCFSAMDYDLWLRLYAHTQKIVRVPEVMAFYRWH
                     GGGQISKTRWKQVLDALRVRRDFVAAHPERVAHLPAARLAELTDGYLLREAYRAYWRR
                     ELDDAQVLLRAALREGAWGVRDLKYILPALLPAPLFRRLVGLAGSTG"
     gene            23577..24377
                     /locus_tag="PKN25_02030"
     CDS             23577..24377
                     /locus_tag="PKN25_02030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV89704.1"
                     /translation="MTTSPAQRVPVLMYHRIGATHNDWERKYCVSPEAFAAHMDTLAR
                     AGWKAVSIDAFFAWLDGGASLPEGAFLLTFDDGFRGVHDHAGPVLHRLGWPATVFLVS
                     ALIGERDAWCEKHNPDGHTYPLMDREQILALRARGFSFHSHTRDHADLPTLDDAALQA
                     QLAGARDDLEALLGAPVDYLAYPYGRYDERVLHQARQAGYRAAFSVQPGFNRPEVDRF
                     RLRRLDVFGTDTPAMLRRKITLGSNDGRLTAALRYNAGRVLARLGIRH"
     gene            24387..25310
                     /locus_tag="PKN25_02035"
     CDS             24387..25310
                     /locus_tag="PKN25_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV89705.1"
                     /translation="MDGSTPFITVALCTHNHADRLARTLETLADLHPPGRPWELLVID
                     NGCTDATPTLLARTAWHPAGVAVRVVREERLGLSNARNRALREARGEYLLFMDDDETP
                     DPEWLRAYEQAMEAFEPDALGGRIEVFFEDGVRPRWLQDELLGFLGKLDHGEARWLDA
                     PGTPIFGGNFAFRRGVFDTIGDFDARLGRMGKANIGGEDTEIYRRLLAHGCRVRWVPQ
                     AIIHHRIQTPKLRRGYFLDLHFRQGRTEGSAKRGSGRRIPPPYLIAQWLRALGRAAGL
                     RLRTGADHSLRMEMNAAYFCGFLLGWMRDRG"
     gene            25354..26316
                     /locus_tag="PKN25_02040"
     CDS             25354..26316
                     /locus_tag="PKN25_02040"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNV89706.1"
                     /translation="MSSFRLSHLHKLLDPEIRAKNLQWLRWRARELYWSAAHPAMPDP
                     VFVIGCSRSGTTVTFETLAASGQYLHFDYELPQLWDGLYGPLNNGWASEAAGPEHADH
                     AHRERALAYFYARLGAGPVLDKTCINTLRVAYLHKLFPRAKFVFIHRDGRDNISSMID
                     GWRLGRTDGGFGLEKFFGPSPVPVEINGGEFREWHFFLPPGWRDYNRASLEEVCAFQW
                     ISANRLALDAARDVPAAQWIQVRYEDIFERPVEMFRTVFERLGLPFDARMEAHCAGLS
                     GRPTSIVKGTPKKQKWKESNPEAIARILPAIAPLMRELGYALDD"
     gene            26300..27202
                     /locus_tag="PKN25_02045"
     CDS             26300..27202
                     /locus_tag="PKN25_02045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV89707.1"
                     /translation="MRSTTEPTVSVVIPAFNAAWCIRRAVDSVLAQDFTDFELIVVDD
                     GSTDDTAEVLRGYGDVLRLVSQPNGGMSRARNAGIRLARGRYIAFLDADDRWLPAKLG
                     RQVALLDQRPELAFCAATASLEDPDGKPVGIWACQGDGTASVAEVFAAHALIAGGASS
                     VLARREPVQALGGFDEALFGAEDTDLWIRLAACGGFACIPEALVVVLKRPGSVSRNRA
                     RMRAGALAMTRKNRHLLPADKQGAYWRALYAGTLCDYAKWACREGDRGAALADLARAF
                     ACAPIGRGRLIASIALAVLAGGRL"
     gene            27199..30396
                     /locus_tag="PKN25_02050"
     CDS             27199..30396
                     /locus_tag="PKN25_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNV89708.1"
                     /translation="MNVATAARSLTILICTHNRADLLARVIDSLESARQPAGWSVRLF
                     VVANACTDGTHEFLAGRSDRADRLALSWIAEPTPGKSHALNRALPLLEDELVAFVDDD
                     HRVDADYLLGVTAAAERWPEADLFCGRIVPDWDGSEPAWVHDEGPYRIYPLPVPRYDQ
                     GMEDFPVDLEGPIPGGGNLVARLPVIGATGPFAIELGPTGHDLGGSEDADWILRALRK
                     GARLHYAPQILQYHYVDSERLTLSYVARKGYQRSQSVTRVRAEFDRVPRYMWRKAAGY
                     ALGLAFSWRLQARRFYLVRLASSMGEISGIRDRHRRKRRRAALPMLGAEAGSAALLVA
                     AALTLALAAVLARHWLGEALLGAGVVGALTSAVLVAKSVRDFSRTGPGLREEILARYR
                     GYVVFAIARLGLAALGLAAFWAFPGTALWITAAEALGREPPLWTTAAGGALTLALATV
                     YAGCRALSQNPGLVIASWQYRTVRIHRLWRALSQRGLDLIARIVLATGIGLVGAIALL
                     RYHQGGSADAGAMLLVTCGYIALLAWAIWEPDGTHAPTPRRRARRNVLMIGSDTLRAD
                     RIGAQREGASITPNIDALAARGTRFGACYVPCARTAPSLISLFTATWPHHHGVRDNYV
                     AGAETRLEDKTLPNILRALGYRTAAVSDWCGADLGKFDFGFDITDLPEDQWNLKYLIR
                     QGPKDIRLFLSLFLHNRLGRHLLPEIHYLGGVPQTGMLGRRARRTLSRLAAGDEPFLL
                     NLFYSTTHPPFASEHPYYTRFSDPAYSGASKFAMARLTEPFEIIRRQGEPREEFDLDQ
                     ILDLYDGCVAQFDDEVGRLLRQLDDSGLAEDTIVVLYSDHGMEFFEHGTWGQGNSALG
                     DFSARVPLIVVDPARPGGQRVDQVVRSVDIMPTLLDLLGAPSVGCDGVSLRPAIADPA
                     TDLHLRAFNETGIWIAPVPGLPEGHLSYPNLLELLDVPDIAAGSLSLRERYRQTVLVA
                     KDRMVRDGRWKLVYQPLEHGRLLSLYDVESDPGCTADVASRHPAEVERLWAQLRAWMA
                     NDPALRGDPRLDLPPTPAATSAARAPEADLAPEMR"
     gene            complement(30349..31965)
                     /locus_tag="PKN25_02055"
     CDS             complement(30349..31965)
                     /locus_tag="PKN25_02055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNV89709.1"
                     /translation="MRSWLPCALLFALSALFLAPALGGSGFWWTDETRHAMGGVFILD
                     LLRDLPLDDPMGYALRYFAQYPALALNWYLPGFYFVEAGFFALFGVSETVAHWTVFAF
                     VLLAAGLWFDWVRTGWNPLMAFMATALFIAVPEWNHWARSVMLEAPAIALLVLVVWTF
                     ERYLDRPAWSRAIAAGLAIAAALAVKQTVALLLPALLAYGLISSRRAALWRVQAVPAY
                     LIAALAIALVALHALKFGNLGLAATVGDSRVEVGQSAARLSLERWLTYPRALIEVWGW
                     PLALLSLAGALLPARQGEGRLALIHAWLLCWYVVMTLVLGGAGNAPRYTLYALPALAV
                     LAARPLFLLRGRRSHDGFLVLLVVMLAFQGWRTLTQPVPRIDGYREVAQAVHERRTGA
                     PVLFAGKHDGSFIFHLRQLDHAREAVVLRADKVLVSLAVHKYFGMSSHVSGIDDIRAL
                     IRRYGIEYVVIERPDIVGVPEFEMLAELLAQADFERVAEFPVHAAGGAEAPERIVLHR
                     YRDHVPAADAEIVIPLPHLGREIRFRRAGG"
     gene            complement(31962..33668)
                     /locus_tag="PKN25_02060"
     CDS             complement(31962..33668)
                     /locus_tag="PKN25_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89710.1"
                     /translation="MSHHRACLPGAGGGCASGGGRGRVAGRTRVLWLAVGLAMLLVAL
                     SRAPLADVHWDAPIYLYQAKRFAETAYLVDVARNAQEIVAQLAGAWPEDESYSEAFWR
                     SSRLGHIALLGAPVAAFGSTDAAITAAHWMFVLAMPLGLLAWCRVSHLAGRALEPGAV
                     GAGGLASSALLFVLSDVHGYLSGNLVSEVLSFGLGAAAVWALLASTATAAGWLAAVSG
                     GLAFLGYTVRVESVWIWLAFMLAYLPFAGAAEARARTLRRFVLAGGTALLGYATYAFV
                     FHPLADPRSYLAFAASLAQRAREGVHAGNLVFVAGGMLWLGAVFALPRFRGSRVVRFG
                     VLWLLLAGLPSWALIASDSAVQTRMLIGLVPPLILLSAAGWTFVLGARQRCAALGLAV
                     VFVLMSRPPIYGWFVEQPGLWRIQRLGAAMFVPRYERVDYVPREMAAISAQVFGGEGR
                     PGILIAAPSVKQEYLNLVRFYGPAYPADAHMALAGDPTNPGRCELRRDRAHEPVRFCT
                     GFGDAAALRTSLVGHRVLHLRRVGDPPLAGAAPLLQTTGFVLEQLGPLPVPDPSPEGS
                     PP"
     gene            complement(33700..34824)
                     /gene="rffA"
                     /locus_tag="PKN25_02065"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(33700..34824)
                     /gene="rffA"
                     /locus_tag="PKN25_02065"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNV89711.1"
                     /translation="MNIPFNKPHMTGKELWYIAQAHANGHLAGDGNFTRQCSRWLEAR
                     TGASKALLTHSCTAALEMAAILADLEPGDEVIMPAYTFVSTANAFVLRGAVPVFVDVR
                     PDTLNIDERLIEAAITARTRAIVPVHYAGVACEMDTIMDIARRHDLLVVEDAAQAVMA
                     SYKGRPLGAIGHMGALSFHETKNIISGEGGALLVNAPGRAERAEIIREKGTNRSQFFR
                     GQVDKYTWVDIGSSYLPGELIAAFLWAQMEEADSITRRRLGIWNTYHQWFAEAERQGR
                     LRRPVIPAHCGHNAHMYYLLLPGLEQRTAFIERLRGLGIHCVFHYIPLHDAPAGRRFG
                     RAHGELSVTENIADRLVRLPLWLGMEDAQAEIIQQVLAAL"
     gene            complement(34821..35243)
                     /locus_tag="PKN25_02070"
     CDS             complement(34821..35243)
                     /locus_tag="PKN25_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNV89712.1"
                     /translation="MSAQATLGQFVRYATVGLASNVVLYLAYLALTATGVGAKLAMSV
                     LYLLGVVQTFVFNKRWSFRHGGMHGPAFVRYCLSYAAGYLLNLVALMVLVDRMGHPHQ
                     LVQGVSIVVLAILLFLLQKFWVFRPNSVPHSHSTGPQP"
     gene            complement(35240..36043)
                     /locus_tag="PKN25_02075"
     CDS             complement(35240..36043)
                     /locus_tag="PKN25_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV89713.1"
                     /translation="MTSAAEAAPSHPLHGRIDTAELRLEWAEAPWDTAVFGHPVVQVS
                     RIEARTGASAADLAPFVAACEVGGVGLASCRLPHDRLRESMLLEEIGFRFIEMVFQPE
                     FGDLQSAGPRDAQGLVVERARPEDLEEIVEVAGGAFRNERFHVDPRLPSRLGDLRYQN
                     WVRSSLEHPSQRLHAVREGAELVAFFVTEQLADGTCYWHLNAVVPQRQGRGYGLRAWR
                     AMMNQARLEGATRVRTSIVARNHRVLNLYARLGFSFPAPSMTFHRVRLA"
     gene            complement(36027..36989)
                     /locus_tag="PKN25_02080"
     CDS             complement(36027..36989)
                     /locus_tag="PKN25_02080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV89714.1"
                     /translation="MSIDISAIVPVYRSQAMLPELHRRLVGALEQIGRPFEIILVEDC
                     GGDDSWSVIEQLAAADPRVLGLRLARNYGQHNALLCGIRAARGELVVTLDDDLQNPPE
                     EIHRLLARLDEGYDVVYGSPQAETHGFLRDQASRITKLALQGAMGVESASKVSAFRVF
                     RARLREAFSAYRSPSVNIDVLLTWGTTRFGSVLVRQDERAVGDSGYTLRKLINHAINM
                     MTGFSVLPLQVASVLGLAFGSMGFLVLLYVLLRYLVDGSAVPGFPFLASIIAIFSGVQ
                     LFALGIFGEYLARMHFRSMERPPYALRSSTAAQGDTETVHDQCC"
     gene            complement(37009..37827)
                     /locus_tag="PKN25_02085"
     CDS             complement(37009..37827)
                     /locus_tag="PKN25_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV89715.1"
                     /translation="MVKVLRAAVLLAGSIAMTGASAGTEADYQALRAWCVQRLQNQNN
                     AAWQAANNPQKYFHFHHFCFGMQWENKVVGSASQKERERYVGSIVNETSYIISHAARS
                     HFLMPEVYFLRGWAQYYGKQHIEAESALLNALQLDPRHARAAAQLATLYRATDRRAKA
                     AEVVRTALKLSPNDFRLRKMGEEFGVELPPLVRPPAPKPAAPDEPSQAEPADAAPATT
                     EATVTQEAATAARSAAQVDQDAGTAEGASQGCRFCPPAEIQKRWRESFGEPQKP"
     gene            complement(37840..38937)
                     /locus_tag="PKN25_02090"
     CDS             complement(37840..38937)
                     /locus_tag="PKN25_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89716.1"
                     /translation="MQPRAFSLFVVGLTLVLVAAAWMLAWWLQPVYGDLTRLGGYAER
                     THGWNEPVLEFDPLPTSFGEWTRPVDILVVGDSFANLRPAQQWQNHLAAMTGWSVHTL
                     DVHRVDVDALRASPLFVEHPPKVVIWNVVERGLAADHAKVEGLCPGSRPAPPLALPPP
                     MAVVAHARAVPRPHALAEANPGFARSWLYRGAMRGLFGVQAGNTVEVELVRADLFSSR
                     ASARLLVYGDDFNKAAWSAEHLRRIRCALADLATRFQADGRTVFMTALAPDKSSAYRP
                     WARDPARLPAERLGELFDGLDVPDARLDLAIRDAVERGVVDVYMPDDTHWSGAGHALA
                     AQVIFERLSRLRAQADVATGEARAQGPEAPK"
     gene            complement(38937..40493)
                     /locus_tag="PKN25_02095"
     CDS             complement(38937..40493)
                     /locus_tag="PKN25_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNV89717.1"
                     /translation="MLFNSPQFIYLYLPIVLVGFFWLAATSQRLAAAWLAAASCFFYA
                     WWNPIYLGLLGASIVFNYGMGVRLARESARPAGQRRRALLVFAVGANLLLLGYFKYFN
                     FFADSARVVFDGHWHFDAIVLPLGISFFTFTQIAFLVDAWRGEAREFNFVHYTLFVTY
                     FPHLIAGPVLHHREMMPQFAHASTYRLDWENIAVGLAIFGLGIFKKVVLADGIAPHAN
                     AVFQAAEAGQALSLLDAWGGALAYTFQLYFDFSGYSDMAIGLSRMFGVKLPLNFDSPY
                     KAGSISEFWRRWHMTLSRFLRDYLYIALGGNRKGPLRRHANLFVTMVLGGLWHGAGWT
                     FVVWGALHGFYLMVNHAWRWLVGRLGLRGGRAWRVLGWALTFVAVVVAWVFFRANSFD
                     AALAMLGAMAGANGVAVPPDWLNALHWRLNRLVGEMGVGAGQLAGQFADAWVWRDLAI
                     LAAIAFFAPNTQQIMSRYRPALDFRGGVDEAGLLRFRIGTPWLVATVLLLATALTRGD
                     AISEFLYFQF"
     gene            complement(40497..41642)
                     /locus_tag="PKN25_02100"
     CDS             complement(40497..41642)
                     /locus_tag="PKN25_02100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV89718.1"
                     /translation="MRNRYLVLTELFLPTKGGTAVWFDEVYRRLGGKEIHILTAQVPG
                     AAEYDRDHPNSVHRLNLQRVGWLRPESLGMYLRFLVCALRLALTRRFDAIHAGRALPE
                     GLVAWLVARLTLRPVVIYAHGEELTGWGRGNKYKAMCFALRHADRVIANSDFTRDTLI
                     GMGVRPERIVLIHPGVDNERFRPGLPAGDLRARLGLGDSARLILSVGRLSRRKGFDTL
                     IRSLPALRARGVDVHLAIVGIGEDADYLDGVAREAGVAERVHRLGHVPMEDLPRWYNA
                     CDVFVLANREIGGDTEGFGMVFLEAAACGKAAIAGRAGGTGSAVLDGVTGLRVDASTG
                     AELVDALARLLDDPPLLESMGQAGLARVRQSLSWQAVAERTRNCLRA"
     gene            complement(41677..42870)
                     /locus_tag="PKN25_02105"
     CDS             complement(41677..42870)
                     /locus_tag="PKN25_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89719.1"
                     /translation="MLAFLVVHRAALRALWNEPVLAQPVLIVESDDWGPGPVADADAL
                     AGLASRLAAVRDQRGRPAVMTLGVVCSLPDGAAMLADGLARYHRRALDAPEFGPMVEA
                     MRAGCGAGVFALQRHGMEHFWPDALLARARVDASLRDWLADPQARSEGLPSHLQSRWI
                     DASVLPSRPLDPAAVQCAVDEEARLLERLFGAAPVVAVPNTFVWDDAVERAWAGSSVR
                     CIVTPGRRIEGRDGQGALMPPTRVIRNGERTADGLMQLVRDDYFEPIRGHRAEQVWQA
                     VAAKRRLGRPVLLETHRESFVGAAAVARAANDELERALRGVLERHPDVRFLSTAELAD
                     ELAAAATGLVTRSLPHRVAVFLARVEHEPALRRFLKYSGLRFALAPVFLIAGVMHGRC
                     AARAR"
     gene            43173..44942
                     /locus_tag="PKN25_02110"
     CDS             43173..44942
                     /locus_tag="PKN25_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNV89720.1"
                     /translation="MLAAEGLAPAQIRVQTSEAGVLACTHPAGSSAVDLRSGSMAVLL
                     GNPRWPDARLATLAHSEGHAAALLDAWRNSGEAALETIQGSFALVLVDSARDRILLAV
                     DRCGAERLCFAAVGGTLVFSTSARAVARHPLVAATIDRQALYEYLYCHMVPSPDTAFA
                     GVEKLLPAQCASFSGGRLERRFYWQLPYAPEVDADEASLRKRFLDLTEQAVARQYDEK
                     RSTGAFLSGGTDSSTVSGMLGKVSGQPARTYSIGFRAEGFDEIEYARITSRHFGTEAH
                     EYYVTADDIVEAMPLIAAAYDEPFGNASAAPTYFCAKMARETGIEIVLAGDGGDEFFG
                     GNERYAKQGVFERYHRTPAALRKLLEPLVMNFPLGDSILPVRKAQSYIRQARIPLPDR
                     LETYNFLHRDPLQEIFEPEFLSAVDTERPLKLARDAYFRSASTEVIERMMHLDHKITL
                     ADNDLRKVTRMCEAAGVEVRFPFLDDDLVEFSGRLTPRHKVDKGRLRVLFKDALRGFL
                     PNETITKSKHGFGLPFGVWLNSHGPLKSLADNALDSLSQRGIVRPAYLDALRKAHASG
                     HASYYGVMIWILIELELWLQHNA"
     gene            complement(45044..46093)
                     /locus_tag="PKN25_02115"
     CDS             complement(45044..46093)
                     /locus_tag="PKN25_02115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNV89721.1"
                     /translation="MDAVKHAVFPQVAGRMPSIDAARAIAIFAVLAIHCDPFIGRVYS
                     LGEVSAGELIDLAGRFAVPFFFVVSGYLLGERCRKSGRDLRVVSGFAGRLAGLVLFWY
                     AFYLLWTPDWNAAWEGGWIRLAYWRAAALFGDGWSLLAGPRAHLWFLPALLIGTLHVF
                     AVMRVKRRVPCLVYFALLYAMGLGAGAYADHPLLRPLSPGVPGGLMCAPLIIATGWWS
                     AGDTVRIGRRVACFLFIGGTVATFVEAWTLLHTYGVPLEGHDFLLATPFQVWGLLNLL
                     RSSPSWAVGTPLPQWGRYTLGVYAGHVAVMEVLGAREGGGLLWEFLKTPLVYLLTLSV
                     VGLLARWPRVRPFFA"
     gene            complement(46275..47237)
                     /locus_tag="PKN25_02120"
     CDS             complement(46275..47237)
                     /locus_tag="PKN25_02120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV89722.1"
                     /translation="MSTSTRSPSVTVVLPTHDRPRLLAEAIASVRAQTWENWDLIVVD
                     DASDPPASIPDDPRCALIRNVRSRGGAEAKNAGAFQARGDVLAFLDDDDLYAPSYLQR
                     AVAALAEDPELDVVFMGVSWFGERSEAGQRAYEQAMGALLSTLHPFSSPRGGVLAFEP
                     AELFRGLLRTVPMAFQRPVVRRSSFERIGPYQADILLWDCDWAIRAALQARCALVTDG
                     LYMQRASGQGFSSKSDRQREHLRSNALMKQRLLEGKLEAEGRAAVRDALVELWFGAAW
                     SSSKGGDQARALEYLWNSARIRLELGQLRLLARILARAIGLVRG"
     gene            complement(47269..48627)
                     /locus_tag="PKN25_02125"
     CDS             complement(47269..48627)
                     /locus_tag="PKN25_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HNV89723.1"
                     /translation="MRDLIVTLAVFGTLPFILRYPWIGILAWSWLSYMNPHRMAWGFS
                     TSMPFAFMVALATFVGILFSREKKEIVWSRETWLMLVFTGWMFLTTAFSWYPGLAWPQ
                     WDKVWRIMLMTYVTLMLIRDRDRVHWLVVVIAFSLAFYGVKGGIFVLTGGSGNNVRGP
                     GGSFIEDRNSIGLALLMTVPLLWYIRLQLKVAWQRLGLLAMSVLTLIAVIGTHSRGAL
                     VGLGAMGVFFLLKARNRLGVLIGIIPVVLVVFYVMPPEWFERMHTIQTYDEDGSAQGR
                     IYAWGNAVYIANSSLLGGGFRAVTGFGGTDSHSNWFGTLGEQGWPGLVMFVLLHIFTW
                     RSATQIIRMVKPHKELTWARDLAAMIQVSLIGYMSAGSFLGLQYFDLFYHLIVIIVVV
                     HELVRRAVGEIAEPRVQARGSSASGLERPLSSAGPTPPDKSHLPQPRVAGGRFASSRS
                     HR"
     gene            complement(48615..49988)
                     /locus_tag="PKN25_02130"
     CDS             complement(48615..49988)
                     /locus_tag="PKN25_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV89724.1"
                     /translation="MSGLYTRLVSGLLFPLHERLKQHDSVALRRGLEDSQWWPRERIE
                     ALQLERLRALLVHARDHVPYYREGFARIGFDPAAIASLAELQRLPFLGKAEIRANTEA
                     LKADDAVGLARFNTGGSSGEPLIFFIGDRRVSHDVAAKWRATRWWGVDIGDPEIVVWG
                     SPIELGAQDRVRALRDRLFRTELLPAFEMSATKLDGFVARIRARRPKMLFGYPSALSH
                     IGLHAERRGIRMDDLGIEVAFCTSERLYDHQREAIERVFGCPVANGYGSRDAGFIAHQ
                     CPQGGMHLTAEDLIVEIVDGEGRVLPPGQAGEIVVTHLATGDFPFIRYRTGDVAVMDT
                     ATCACGRGLPMLREIQGRATDFVVARDGTVMHGLALIYVLRDLPQVAGFRIVQESLEH
                     TRVQVVPGVGFDADTVGRIEHGLAARLGEGVRIDVEQVAEIAPEKSGKYRYVVSKVDA
                     SEVSCAT"
     gene            complement(50005..>50753)
                     /locus_tag="PKN25_02135"
     CDS             complement(50005..>50753)
                     /locus_tag="PKN25_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HNV89725.1"
                     /translation="ILALFWIGSRWQEDEEEAPAMPVGSARSSGVRTGGLGWLAVAVV
                     AVGLWVPVLGHLERQGEQGPVRFAALEGQGAWQRTEAGELPPWSPSYSGMRDTFRSTW
                     RAGSVPVGVYIGYYRDQGPGRELINSENRVLISKDPVWRMTAAGPVRVQLGDQAQAWR
                     TLEMASEHARMVVWYAYWIGGRWTTSDHLAKVYLALSRLRGEGDDSAVVMLHAPQGQG
                     GHAGTIAALEDFAREMGGPLQAMLDRTANP"
BASE COUNT         8156 a        18464 c        16852 g         7281 t
ORIGIN      
        1 ttgcaccatc tcgaccgcac gacgcacgtc gtcggcctct tcgatcacac tcttgttctt
       61 cattcggcac tcctcagcgc gcatcggccg ccgccttgcc ggcggcgagg atcgccgcgt
      121 gcaggcccgc gctggcggca tcccgccctt cgcccgccat gagccgggcc aactgggcat
      181 cgtcgaagcg gaagcagggc agcagcgtcg gactggacgc catcctgacc agcttggccg
      241 ccgacatcga ctcgatcagg tccgcgaagg cctcgccgat cccgagccgg aacagcgcgg
      301 tcgagcggtc gtcgcgcagc atctgctgtg ccagcatcag gtaggcgagg ttgagttcgc
      361 ggatttcttc ctggaagtcg gggcgatcca tggttttcca tcccgcacgg ggatcaacgt
      421 ctgcaagtta aagtagtttc tcaggagcgg caagccgctc cccccggctc cgtgccgcat
      481 ttcacgcaca aggtgtcgcc cccgaacaac acctgccggc ggggcggccg cgccttcccg
      541 ccggcattca gcccgcgccg gactggagct ggagtgcggc gatgcagttg cggccacgct
      601 gcttggcacg atacacgctg tcgtcggcac acttgatcag tgcatcgacg ctgcccatgg
      661 cgggcacgcg catggccacg ccgacgctgg cagtgaggaa gatctgcggg ccgccggtct
      721 cgaccggact ggcctgcacg gccacgcgca ggcgctccgc gaccagcatc gccgccttca
      781 ggtcggtatc ggggcacagc accaggaact cgtcgccacc ggtacggcag atcaggtcct
      841 gggcgcgcag cgcgccacgc agggtcgccg cggcctggcg cagggccatg tcgccgacgt
      901 cgtgcccatg ggtgtcgttg atggtcttga agccgtcgag gtcgacgatc atgaccgaga
      961 aggcgcgacc gtgacgcgtc gccgtggccc actcggcctc catcgcctcg atcgcccgac
     1021 ggcggttggg cagaccggtg agcgggtcgg tcagcgcggc ctcctgcagg cggcggttcg
     1081 agatcgacag ctcggcggcg aaattgcgca gttcctcgcg ctcgtgctcg agttcctgct
     1141 gcagcaccaa tgtgcgcacg caggcacgca ggcgcacggt cagcgccgca cgtcgcagcg
     1201 gacggacgag gaagtcgtca accccggcgc cggaggcggc cagcaggcgc gcctcgtcgt
     1261 cgctgccgag catgcagatc acgtacacgc cgcgtccgag gcgcatgtcg cgcaggccgc
     1321 gcaccacccg ttccccctcc tccgaccagt ccgcgaccag cagttgcggc tggacgtcga
     1381 gcgcggcctc gagcgcggcg tccgcgcccg cagcggtgaa cgggacgatc cccgcggctt
     1441 cgagctcgcc gcgcagcagc tcgcggtcct cgcgcgcgcc ggccacgacc aacgcacgca
     1501 tcggctgcgg accagcggcc tcggcggcca ccttgggcgc ctcgccaacc gcatccaccg
     1561 ccgcgctgcc cacctcggcg aagcggggcg cgtcctcggt gcggatctgc agcagcttgc
     1621 cccactccgc ccagtcgcgc ccgacgcgct cgcacagcga gacgaactcg ccgcgcccga
     1681 ggccgaggcg gcccgccagg cgcagcatcg gcgccatcag acgcacgtgc tcggaatcgg
     1741 gcgcgaggca cagctgcgcc accgcgcgcg ccagcacgag gcactgcacg atcccggcct
     1801 cgcgcgaatc ctccgcgtag ccggccagtt cgggctgttc gtggaagcgc accggttgca
     1861 ccagcgcgtc cggcaccccc cagtcggcaa gcatggcggc accgagctcg cggtggttca
     1921 tcgcgaaggc ctggtcctcg agcaccacct gctgcagggc gggatcgcgc ttgagttccg
     1981 cgagcaggcg ggagaaggcc tcgggataga gggtcgcgag cgagagctcg cccacgcgcg
     2041 cgagcaggcc gaggctgaac gcctcgtcgg gtgcgaccac gcgcacgcgc tgggtgagcg
     2101 cctgcatcga gatcgccatc agcagcgcgg aggaccagaa acgcgggtag tcgaagccct
     2161 ggcaggtacc cttgcggtag gtcgacagca gcgagaagcc cagcgccagc gtgcgcacgg
     2221 cgggcaggcc gagcaccatc agcgcttcct gcaccgacac gatcgcgcgc cggttctccg
     2281 cgatgaggcc attggccgcc ttgatcaggc gcccgacgaa ggcgggatcg cccttgatca
     2341 cgcggccgag ctcggccagc gagacgtcgg gcgcctgcgt gagccggatg atcgcgagcg
     2401 ccacgccgcg cggcgaaggc aggtctcccg ccgccttgag ctgcgcatat cgcttcatgt
     2461 cgatcggttg cggcatccct ttacactccc tcgctcgctg tcgccggccg attctggcac
     2521 agagcccgct tcatcggccg actggcgtca ttctttaggc cgtgctgccg gcagacgggt
     2581 caaagcaggc tagaatcgcc gaaaaccatt gtccgcgacc atgaaatact gctcgaattg
     2641 cggcgccacc gtcgccttcc gcatcccgcc cggggacacc cttccccgcc acgtctgcga
     2701 gcagtgcggc accatccact accagaaccc caagttggtg gtgggcgcgc tggccgagtg
     2761 ggaagaccgc gtgctgctgt gccggcgtgc gatcgagccg cgccacggca tgtggacgct
     2821 gccggccggc ttcatggaga acgaggaaac caccgcccag gccgccgccc gcgagaccct
     2881 ggaggaggcc tgcgcgcgca tcgaggtggg cgagctgtac acgctgatcg acgtgccgca
     2941 catcagccag gtccacatca tctaccgcgc ccgcctgctc gacctcgact tccgccccgg
     3001 cgaggagtcg ctcgaggtcg cgctgctgcg cgaggacgaa atcccctggg agcggatcgc
     3061 cttccgttcg atcgcgatca gcctgcgcca cttcttcgac gaccgtcgca gcggccgctg
     3121 gcagctgcac accgccagcc tcgccccgcc gccaccggat tggcgctgag gcccggaggc
     3181 ccggccgata catgctcccg catcagggtt gatgcgggac tcgggcgcgg aggataatcc
     3241 cgcaattccc ttccgcgcgc ggatcatgac cagctacatc ttcgtcgtca tcggcgccgt
     3301 cctggtgaac aacgtcgtac tcgtccgcat cctcgggctg tgcccgttca tgggcgtgtc
     3361 gaaaaagctc gagaccgcca tcggcatggg cgcggccacg accttcgtgc tcaccgtcgg
     3421 cgccggcacc agttacctga tcgaccacta cctgctgcag cccttcgatc tcggctacct
     3481 gcgcacgctg gccttcatcg tcgtgatcgc ggcgatcgtc caactcaccg agctgatcat
     3541 ccagaagacc agcccgctgc tgcaccaggt gctggggatc tacctgccgc tgatcaccac
     3601 caactgcgcc gtgctcggcg tgcccctgct caacgtcggc ttgaagcacg acctgctcga
     3661 atccctgttg ttcggcttcg gctcctcgct cggtttcacc ctcgcgctcg tgctgttcgc
     3721 cggcatccgc gagcgcctgg acggcgccga cgtgccgatc gccttcaagg gcaccgcgat
     3781 cgcgatgatc accgccggtt tcatgagcct cgcgttcatg ggcttcgccg gcctcgaccg
     3841 cttccactga ggcccgctcc accatgatca ccgcgattct cgtgatggcc ggcatcgcca
     3901 tcgtgctcgg cctcaccctg ggctacgccg cgatccgctt caaggtcgag ggcgacccgc
     3961 tggtcgagaa gatcgacgcc atcctgccgc agacccagtg cggccagtgc ggcttccccg
     4021 gctgcaagcc ctacgccgag gcgatcgcca acggcgaggc cgacatcaac cagtgtccgc
     4081 ccggcggcga ggagggcgtg cgcaagctcg ccgacctgct cggacgcgag ttcaagccgc
     4141 tctccgaaga gcacggtgtc gagaagccga agtcggtcgc ggtcatcgac gagcaggtct
     4201 gcatcggctg cacgctgtgc atccaggcct gtccggtgga tgcgatcgtg ggcgcggcca
     4261 agcagatgca caccgtggtc gagccgctgt gcaccggctg cgagctgtgc gtggcgccct
     4321 gcccggtcga ttgcatcgcg atggtgccgc tggccgagac cgtgcacaca tggaaatgga
     4381 aatacccggt ggtcgagatg cggagggtcg cctgatgctg cgtgagcttt ttcgcttcca
     4441 tggcggcgtg aagcccgcaa ccaacaagag cgcctcgacg acctcgccga tcgctaaggc
     4501 ccccatcccc gcgcggctgg tcgtccccct gcaccagagc ctgggcagcc ccgccatcgc
     4561 cctggtcgag cccggccagc gcgtgctcaa gggggaacgc atcggcgcgg cgggcggacg
     4621 cctgtccgcc gcgatccacg cacccacctc cggcgtggtg cgtgcggtcg agccggcgct
     4681 ggtcccgcac gcctcgggcc tgcgcgaacc cgcggtgatc atcgagagcg acggcgagga
     4741 ccgctgggcc gagcccgaac acatcaacta ccgcatcacc ccgcccgacg aggtgcgcga
     4801 cttcgtgcgc gatgccggcg tggtcggcct cggcggcgcg gtgttcccca cccaccagaa
     4861 gatgcgcccc ggcagcggcg gcaagacccg cacgctggtg ctcaacggcg ccgagtgcga
     4921 gcccttcatc acctgcgacg acatgctgat gcgcgagcgc gccgacggca tcgtctccgg
     4981 ggccgcgatc ctgcgccaca tgctgcaggc cgaggaagtc ctgatcggca tcgaggacaa
     5041 caagcccgag gcgatcgcgg cgatgcggca ggcggtcgcg gcggcgggcg agcacatgga
     5101 agtggtcgcc gtccccaccc gctatcccgc cgggggcgcc aagcagctga tccgcgtgct
     5161 caccggcatc gaggtgccgc acggcgagcg ctccaccgac tacggggtgc agtgcttcaa
     5221 cgtcggcacc gcctacagcg tccacgaggc gctcgagctc ggccgcccgc tggtgtcgcg
     5281 catcgtcacg gtcgcgggca acgtcgagcg cccgggcaac tacgaggtcc tcatcggcac
     5341 gccgatggac gagctggtgc gcctgtgcgg cccgcgcgcg gacacccgct tcctcgtcat
     5401 gggcggaccg atgatgggcg tgcgcatgcc cgccttcgac gtgccggtgg tgaaggcgac
     5461 gaactgcgtg ctcgcggccg acgacaagct tttcccgccg gctccgcccg agcaaccctg
     5521 catccgctgc accgagtgcg ccaaggcctg cccggtcgag ttgcagccct tcgagctgta
     5581 ctggttcagc cgctcgaaga atttcggcaa ggcacaggaa taccacctct tcgactgcat
     5641 cgagtgcggc tgctgcgcct acgtgtgccc ctcgcacatc ccgctggtcg actactaccg
     5701 cttctccaag ggcgagatct gggcgcgcga gcgcgagaag gcggccgcgg accaggcacg
     5761 cgagcgcttc gagttccgca acttccgcca ggagcgcgag aaggccgaga aggccgccaa
     5821 gctcgccgcc aaggcggccg agacgcgcgc caagctggcc gaggccggca cggccgaagc
     5881 gccggcggcg gccgaggccc ccgccgcgga agatcccaag aaggccctga tcgccgccgc
     5941 gctcgcccgt gccaaggccc agaaggccga gatccagccg cagaacaccg acaacctcag
     6001 cccgcagacg caagccgaga tcgccgacat cgaggcccgc cgcaaggcgc tcgaggcggc
     6061 cgcggtcccg gcttcgaccg cacccgcgcc gcaagccgcc gacgcgacgg acctccccga
     6121 cgagcctcgc ccctgagccc cgcatgatcc actctcccta cgtccgcaag gcggtcagcg
     6181 tgcagcgcac gatgggcctc gtgctcctcg cgctgatccc cggaatcgtc gcccacgcac
     6241 tgctcatcgg ccccggcgtg ctggtgaacc tggcgatctc cacgatcacg gcggtgggcg
     6301 cagaggcgct gatgctgcat ctgcgcaagc gcccggtggc gatcgcggtg ggcgacctct
     6361 cggccgtcgt caccgcctgg ctggtcacct tgtgcttccc gccgatcgtg ccgtggtggc
     6421 tcacggtgtg cggcgccctg gtggcgatcg tggccgtcaa gcacctctat ggcggcctcg
     6481 gccagaaccc gttcaacccg gcgatgatcg cctactgctc gatgatcgtc gccttcccgg
     6541 cgctgatgtc gcagtggccg ccggccggcc agctgtcctt cgacacccag cttgcgctga
     6601 tcctcggcgg agcgcgcgac atcgacggca tcaccggcgc caccgcgctc gacgccctgc
     6661 gcaccgggct gcgctcggga acccagaccg tcgatgcggt gctgcagggc tcggccttcg
     6721 gcgcgctcgg cgggcgcggc tgggagtgga tctcggtcgg ctatgtcgcc ggcggcctct
     6781 tcctgctcgc caagcgcatc atcacctggc acatgccgct cgccttcatc ggcgtgctcg
     6841 ccggcatctc gaccctgttc tggctgctcg atccgggccg cttcgcctcg ccgctgttcc
     6901 atctcgccag cggcggcgcg atgctggcgg ccttcttcat cgtcaccgac ccggtctcgg
     6961 gtgcgaccac cccgcgcggc aagctgctct tcgccggcgg catcgcgttg atcgcctggc
     7021 tgatccgcaa cttcggcgcc tacccggagg gcatcgcctt cggggtgctg ctgatgaaca
     7081 tgtgcgtacc cctgctcgac atgaagaccc agccgcccgt cttcggtcac cacaaggacg
     7141 cgaagggagg cggcaaatga gcgcgcgcta ttccgcgacc cgcaccgccc tgcgcaccgg
     7201 ggcgatcatg gtggtgttca cgctggtgtt caccgccttg atggccgcca cccaccgtgt
     7261 cacccagccc gcgatcgagg cctcggcaca ggatgcccag cgccgcctga tcgacgaggt
     7321 gctgccgccc ggcagctacg acaacgacct gctcgccgac gtggtgcgct tcggtccggc
     7381 gccgcagatc ggcatggacg aaggcgggcg ggtgtggcgc gcgcgcaagg gtggcgagcc
     7441 ggtggcgctg atcgccgagg gcgcagccac cgacggctac tccggacgca tcgcgctgct
     7501 ggtggcggtc aataccaacg gcctgctgtc gggcgtgcgc gtcacccagc acaaggaaac
     7561 cccgggcctg ggcgactaca tcgacccgaa gaaggaccgc cgcaaggaca tgccctggat
     7621 cggccagttc gcgatcgcca cctggaagga cgtgaacacc gccgcatgga ccgtgcgcaa
     7681 ggacggcggc accttcggct accgcaccgg cgccaccatc agcgcccgcg ccgtggtgcg
     7741 cgcggccgga cgcagcacgg cgttcgtggt cgaacgcatg gacgcgctct tcgccgcccc
     7801 ctccggcggc gtgctgggag aatgagcatg agcccgcaac aattccgcga gatcgcctgg
     7861 aacggcctgt ggaagcagaa caccggcctg gcgcagctgc tcggcctgtg cccgatcctc
     7921 gcgatcagca ccagcatggt caatgcggtc agcctcgggc tggccaccat cctcgtgatg
     7981 gcgatgtcca acctcgccgt gtcggcgctg cgcaacttca tcccctacga gatccgcatc
     8041 ccggtcttcg tcctgatcat cgccgcgctg gtcaccgtgg tggatctctc cttcaacgcc
     8101 ttcttccacg acctctacct ggtgctcggc atcttcatcc cgctgatcgt caccaactgc
     8161 atcgtgctcg cgcgcgtcga ggcctatgcg gccaagaacg acccgctcac ctcgacggtg
     8221 gacggcatca tgatgggcat cggcctggtg tgggtgctcg cggtgctcgg cggcatgcgc
     8281 gagctgctcg gcgccggcac gctgttctcg ggcatcgaga tgatcctccc cggcgcctcg
     8341 gcgtggaacg tgttcggcga cggctacccc ggtttcctga tcgccatcct gccgcccggc
     8401 gcattcttcg cgctcggcgt gctgatcgcc gcattcaacg ccatcaacgt gcgcctggcg
     8461 gcgcgcgcgc ggcgcaatcc gccacccgcg ccgacacaga cagccgagcc gaaggaagac
     8521 gctgccgagc cggccgccgc gggctgagga cgcgcatggc agcgatgaag cgcgaggcga
     8581 tccgcgagtt tttccgccgc ctgcacgagg ccgacccgca cccgcagacc gaactcgagt
     8641 acgcgtcgcc ctatcagttg ctggtcgcgg tggtgctgtc cgcccaggcc accgatcgcg
     8701 gcgtcaatct ggccacgcgc aagctgttcg cgctcgcccc cacgcccgag gccatggtct
     8761 cgctcggcga ggaaggcatc gccgagtgca tcaagagcat cggcctgttc cgcaacaagg
     8821 cgaagaacac gctcgcgctg tcgcgcctgc tgctcgaacg ccacggcggc gaggtgccgg
     8881 cggtgcgcga ggcgctcgaa gccctgcccg gtgtcggccg caagaccgcc aacgtggtgc
     8941 tcaacaccgt cttccgccag ccggcgatgg cggtcgacac ccacatcttc cgcctcgcca
     9001 accgcaccgg gctggccccg ggcaaggatg tgctggaggt cgagaaggcg ctgctgcggc
     9061 gcgtgccgaa ggactacctg ctcgacgccc accactggct gatcctgcat ggccgctacg
     9121 tgtgcaccgc gcgcaagccc aagtgcggcg agtgcggcgt gcgcgacctg tgcctgtgcc
     9181 gcgacaagac cgactgaaac ccggcgcgga cgcccgccgg cgcgcgctgc gcccgcaccg
     9241 cacccgcgcc cgaacacccc gcgcccgagt gcgcaagaga accgaggacc cgacatgttc
     9301 aaccccagcc gcgaccaggt gcgcagcttc ttcatcgaca cctggcgcaa ggcgcgcgcc
     9361 aaggaagtgc tgacgccgat ggagaccatc gccgccgaca tcatcgcgct ccaccccgag
     9421 taccacgccg tggtcgagga cccggaggcg gtgcaccgcg actttccgcc cgaggccggg
     9481 cagatcaacc ccttcctgca cctgtcgctg cacctggcca tcgaggagca gctttcgatc
     9541 gaccagccgc ccggcatccg cgccgccttc gaggccgcct gccagcaccg cggcgaccgt
     9601 cacgacgcca tgcacgacgc gctcgagtgc ctcggcgaga tgctcttcga cgcccagcgc
     9661 aagggcaccc cgcccgacgg cgcggcctac gtcggcgcgc tgcgcaagcg cgccgggctg
     9721 gcgctctgaa cgcagcccga tcggacgccg ttcgccctcc gcgcctcgcc ctgcacgcgg
     9781 gcgaggcgct agaatgatcg ctttcggacg aatggatcgg gcctgcctcg acgaggcccg
     9841 gggccgatcc cggcccacgc cccgcccccc tcattcccgc cccccctttc ttcctcgaag
     9901 aatccccgct ccccaggagg tctccatgcc cggtctgctc acccacgtcg atcccgatgg
     9961 cctgctcgaa tactcggtgg tctataccga ccgctcgctc aaccacatgt cgcaggcctt
    10021 ccagggcgtc atgcgcgagc tctcggcgac gctgaagaag gtctaccacg cccacgccgt
    10081 cgccatcgtc cccggcagcg gcaccttcgg catggaggcg gtcgcgcgcc agttcgccac
    10141 cggcaggaag accctggtga tccgcaacgg ctggttcagc tatcgctgga cgcagatctt
    10201 cgagatgggg aacatccccg ccgaatccgt ggtgctcaag gcccgccagg tgcgcgacga
    10261 ggcgcaggcg cccttcgtcc cggcgccgat cgacgaggtc gttgcggcga tccgccagca
    10321 ccgccccgac gtggtgttcg ccccgcacgt cgagacctcg gccggcatga tgcttcccga
    10381 cgactacctg cgcgcggtgg ccgacgccac ccacgcggtc ggcggcctct tcgtgctcga
    10441 ctgcatcgcc tcgggcacga tctgggtgga catggacgcc accggcgtcg acgtgctggt
    10501 gagcgcgccg cagaagggct ggagcgcctc gccgtgctgc gcgatggtca tgctgagcgc
    10561 cgccgcgcgc gagcgcatcg agggcaccac cagcaccagc ttcgcctgcg acctcaagaa
    10621 gtggctgcag atcatggaag cctacgaggg cggcgcccac gcctaccacg ccaccatgcc
    10681 caccgacggc ctggtcacgc tgcgcaacgt gatgcgcgag accgaggcct acggcttcga
    10741 caaggtcaag gccgagcagg tggaactcgg cgctggcgtg cgtgcgctgc tcgaggcgcg
    10801 cggcttcccg agcgtggccg ccgcgggctt ccaggccccg ggcgtggtgg tgagcttcac
    10861 caccgacgac ggcatcaaga ccggcgccaa gttcgccgcc gccggcctgc aggtcgccgc
    10921 cggcgtgccg ctcgcctgcg acgagcgtcc cgactacaag agcttccgcg tcggcctgtt
    10981 cgggctcgac aagctgcaca acgtcgcgcg caccgtcgcc acgctggaga aggcgctcga
    11041 tcaggtcggc tgatcgggct cgccccaccc gaccgggggg ccactcgccc gagacccgac
    11101 gccgcatgcg cgccagtccg ggcgcatcag ctcaccggca aacgccaccc ttgcggtggc
    11161 gtttgcgcgt cagcgtgtca gggcacattg cgcgcgcagt cgttcgacgg tgtcgggctc
    11221 gccgcgcagc ctgaagaccg ccccttcatc ctccgccctt tcctccagca cctcgcagtt
    11281 ggcgtagatc tccttgcgca gctgctgcgc cgaccacggc aagagcagct ccgcctcgac
    11341 cagatcctgc tggaagaacg ccacgatcgt ctgccgcagc ctggccacct catccggccg
    11401 gcgcgcgctc atgacgatgc agccggggta tttcgcgcgc agcgcggcct cgcattcggc
    11461 ctgcgccgca gcgtcgccga catggtcgat cttgttgaag acacggatgc gcggcagctc
    11521 gtcggcgccg atttcctgca ggacgctgtc ggtgacctcg agctggcgct cgaagccggg
    11581 atcgctggcg tcgatgacgt gcagcagcag gccggcgtcc agcgcttcgt ccagggtgga
    11641 cttgaacgac gccaccaggc cgtgcggcag gttcttgatg aagccgacgg tgtcgctgac
    11701 gagcacgcgc ggcacgctct cggggtagag cgtgcgcacg gtggtgtcga gcgtggcgaa
    11761 gagcttgttg gcgaccagca cctcgctgcc cgtgagcgcg cgcatcagcg tggatttgcc
    11821 cgcattggtg tagccgacca gcgccacgct ggccgggctc tggcgaccct ggcggcgcgc
    11881 gcgctgggtc ttgcgctcgg cctccatcgc gacgatctcc agttgcagct cggcgatgcg
    11941 gtcgcgaatc ttgcggcgat cgagctcggt atgcgactcg ccggcaccgc gaccgcccac
    12001 gccgctgcgc tgccgtccct gcggcccggc aagcttggcg gcttcgcgca ggcgcggcgc
    12061 catgtagccc aggcgggcga tctccacctg cgccttggcc gcgcgcgagc gcgcgttgcg
    12121 gtggaagatc tcgaggatga ccatggtccg gtccatcacc tcgcagccgg tctcgagctc
    12181 gaggttgcgc gcctgcgagg gcgagatctc gtggtcgacc agcagggcct cgatcgcgcc
    12241 ggtgcgcggc acggtgtccg tcggcgccgc gtcgaagccg gcctgcgcgg ccacgaactc
    12301 gcggacctcc tgacgcttgc cgacccccag atagccggtg gtgtcgaaac ccgcacgctt
    12361 ctgggtgaag gtgtggacca cccggtagcc gagggttttc gccagttcgc gcaactcggc
    12421 gagcgaagct tcgaactcgg catcgctgac gttggggcgt tgcaccgagg cgacgatggc
    12481 cttccggggt tcgtcggcga ggtctgtttg cattcagggc gcttctatcg agggatgagg
    12541 ggtgcgatgg taacccgccg cgccactgcc ctgcgacgat cgccgcccca tccccgccgc
    12601 cgctcagcgc ccgccgcccg catccaggtc ggtgaggatg gggcaatccg ggcgtgcgtc
    12661 gccatggcaa tgcgccgcaa gggtgcgcag cgtatccacc atcccctgca gctcggcgat
    12721 cttgcgttcg agcgcggcga cgtgcccgag cgcgatgcgc ttgacgtccg cgctcgcccg
    12781 cccctggtcg cgccacagcg cgagcaggtc gccgatatcc gccagcgaga agcccaggtc
    12841 gcgcgcgcgg cggatgaagc gcagcacgtg cacgtcctcg ccggtgaaga agcggtagcc
    12901 cgcgtccgtg cgccgcgccg ccggcagcag gccgatcgac tcgtagtagc ggatcatctt
    12961 cgccgacacg ccggatgcgg cggccgcctt gccgatgttc atgctgttct ccatgtgccg
    13021 ctcgcgcctc ccagtcgccg gcatccaagc ggagtgcccg gccatgcgat gcaccgacgc
    13081 cctgcgcatg ccgcgtagga gcgacgcaag tcgcgattgc agcggcggaa acaccgcggc
    13141 gcggagagga gcgccggatc gcgactcgcg tcgctcctac ccaaaccacc gtgcatccaa
    13201 agcactgcga cttcccgcac gcagcttgat gcggctcgcc gcggctcgtg gcctgcgctc
    13261 acccggccgt ctgcggcgcg tcggcgccga agcggcgcag gcgcagcgcg ttgccgagca
    13321 cgaacacgct cgacatcgac atcgccgccg cggcgaacac cggcgacagc agcgcgccga
    13381 aggccgggta gaacaccccc gcggcgagcg ggatcagcgc gctgttgtag gcgaaggccc
    13441 agaacaggtt ctggccgatg ttgcgcatgg tcgcgcgcga cagcgcgatc gcgcgcggca
    13501 cgcccgtcag gtcgcccgac atcagcacca cgtcggcgct ctcgatcgcc acgtcggtgc
    13561 cggtgccgat cgcgatgccc acgtcggcct cggccagcgc cggcgcgtcg ttgatgccgt
    13621 cgccgacgaa ggcgacgcga cggcccgccg cctccccgtc accagcgcgg cccgccagcc
    13681 tgcgcagcgc agccaccttg ccgtcgggca gcacctccgc gacaacctcg tcgatccccg
    13741 cctggcgggc gatcgcgcgc gcggtgcgcg cgttgtcgcc ggtgatcatc gcgaccttca
    13801 gccccatgtc gtggagcgcc ctcaccgccg cgaccgagct cggcttgagc gggtcggcca
    13861 ctgcaaggat ggcggcgagg cggccgtcga tcgcggcata gagcggcgac ttgccctcgt
    13921 cggcgaggcg agccgcggtg gcggcgaaca cgtccggctc cagccccagc cggcgcatca
    13981 ggcggtcggc gccgacctcg acgcgctgcc cgcccacccg tgcgcgcaca ccgtagccgg
    14041 gctcggcccc gaagccctcc gcggcggcga tatccagccc ttccgcacgc gccgcgacga
    14101 cgatggcctc ggcgatcgga tgctccgagg cacgctcgac cgccgccacg catgcgagca
    14161 cgtcggcacg cgcaaagccc tcggcaacca ccaggtcggt gagctgcggc ttgccgacgg
    14221 tgagcgtgcc ggtcttgtcc agcgccacca cggccgcgtc tcgcagcagc tgcagcgcgt
    14281 cgcccttgcg gaacagcacg cccatctgcg ccgcgcgccc ggtggcgacc atgatcgagg
    14341 tcggcgtcgc cagccccatc gcgcacgggc aggcgacgat cagcaccgcc accgcattca
    14401 ccagcgcgaa ggtgagcacc ggcacatccg ccggaagcca ggcggccccg aacaacagcc
    14461 aggcgaggaa ggtgagcgcc gccaccccca tcaccaccgg cacgaacacc atcgtcacct
    14521 tgtcgaccag ggcctggatc ggcaacttgg cgccctgcgc cgcctcgacc atgcggatga
    14581 tctgcgcgag cacggtgtcc gcccccaccc gggtggcgcg gaaggcgaag gcgccgcggg
    14641 tgttgagcgt gccgccgacc acggtcgcac cggcgccctt ctccaccggc accggctcgc
    14701 cggtgatcat cgactcgtcc acgaaggacg caccctcgac cacctcgcca tcgaccgcca
    14761 ccttctcgcc cgggcgcacc tccaccacgt cgcccaccgc gacctcggcg atcggcaact
    14821 ccaccacccc ggcgtcgcgc cgcacgcgcg cggtgcgcgg ctggatgcgc atcaggcggc
    14881 ggatcgcgtc gctggtgcgg ccgcgcgcgc gcgcctcgag caggcgcccg agcaggatca
    14941 gggtgatgat caccgccgcg gcctcgaaat acacattgac cgtacccgcc ggcagcagcg
    15001 cgggcgcgaa caaggccacc accgagtagc cccaggccgc cgaggtgccc accgccacca
    15061 gcgcgttcat gtccggcgcg ccgcgcagca gcgcgggcac gcccttgcgg aagaagcgca
    15121 gcccgggccc gaacatcacc gcgctggtca gtacgaactg cagcagccag ctctcgcggt
    15181 ggccgatcgt ggccatgacc cagtcgtgca tgccggggat caggtgcgcg cccatctcga
    15241 gcacgaacac cggcagggtg agcgcgagcg cgatccacac cgcacgtcgc agcgccgcct
    15301 cctcggccgc ggcgtcggcc tcgcgcaccc cctcgcccac ctccgccgcc ggacgcgcgc
    15361 cgaagcccgc cttctgcact gcctccacca gggccgcggt cggcacgccg ccggcgagct
    15421 cgacgtgggc gcggcgcgcg gccaggttca cattggccgt caccaccccg ggcacgcgct
    15481 gcagcacctt ctcgacgcgg cccacgcagg ccgcgcagct catgccctcg atgtcgagct
    15541 cgagggtgtc gcgccgcacc gcgtagccgg ccttatcgac cgcggcgatc agcgccgaca
    15601 gcggcaccgc cgccggagcc gtcacggtgg cggtctcggt ggccaggttg acgctggcgg
    15661 cctgcacgcc ctcgaccttc agcaaggcct tctcgacgtg ggcgacacag gaggcgcagt
    15721 tcatgccggc gatggcgagg gtgcgcaccg cagcggcggg cgagacagtc cggggggtgt
    15781 tcatggtgtc catccagtac ggaaccggga tagtccgcac tgtagacctt gccaacgtgg
    15841 gaaggtcaac ccgacctccg cggtcgccac gccaagcggg ctcgtgcccg tgaaagcctg
    15901 cgccacctcg gccggcgccg caggtgcgga gcccgagcga agtcgcaatc gaccccgcgc
    15961 gcccctcttc gcatgcgtcc cggcgcgtgt agaggccata attcacggct tgttcacgcc
    16021 ttcccgggca caatgcgggg caacattccg cccgcttagg cctcgttccc atccagatga
    16081 gtacatcgaa ctcgattcgc agcggcatca agtggctcgt caccggcagc ctcggcggtc
    16141 aggtgcttca gttcgccttc ggcatcgtgc tcgcgcgcct gctcgtcccg gaggatttcg
    16201 gcttcatcgt caccatccag atcttcacgg gtttcgtcgg cctgatcagc ggcggcggca
    16261 tggggcaggc gctggtgcag tccaaagacg cgcaagcgcg cgatttcgac gtggtgttca
    16321 cggcgcagtt gctgatcggc ctcctgatct tcgccggctt ctacgtcgct gcgccctggt
    16381 tcgcgcacac cttcggcgat gcgctctacg ccgacctcgt ccgcgtctcg gcgatcagct
    16441 tcctcctgcg ccccttcgtg aacacgcgtg cgagtgcgct gcaccgcgac atgcgcttca
    16501 aggagcgctc cgtgatcggg ctcgccgtag cggtcctcac cggcctcgcc agcatcgcgc
    16561 tcgcggtggt gggcgccggc ccgtggagcc tgatcctgtc gggcatcctg ggcagcctga
    16621 tcaccctcgc catgctcgac cgcgtgagcg accgccgccc gcagctgtgc ctggacctcg
    16681 aggtggcacg acgcttcggc gcctacgggg tcaaggtttc gctcaacgac ctcgccgcct
    16741 atttcaccaa gcaggccagc aacgcaatca tcagccgggt tgcggggccc tcggcggtcg
    16801 gtctcttcaa caagggcgag agccttgcct ggctgccctt ctcgaccgtg agcgctgcgg
    16861 tctacgacgc ggtcttccgc gcgatggcga aggcgcagga ccgccccgac gaggtcaagt
    16921 atctcttcta tcgcatgatc ggcctgatgg tcgtctacac gctgccgatc tatatcggcc
    16981 tggcgtggat ggcggagccc ttcatgctct tcgtctatgg cgcgaaatgg gttccgtccg
    17041 ccgatccgct gcgcatcgtc tcgctcgccg gcctgctgat ctgcatcggc cacccctgcg
    17101 gcgcggtact cgcggcgatg aaccgcctcg gccgcgaagt gtggattcac atcgcgcagg
    17161 gcgtgctggt atccgtcgcg tgctactacg gcctgaagca ctcgggcatc agcggagccg
    17221 catggggcgt cttcgccggc atcgccttct ccaccccggt gatgtactac ctcgcgacgc
    17281 gctgcttccc gagccgtctc ggcgacctgt ggcgcgcgct cgcgcccggc ctcggcctca
    17341 acctcatcct gctggcggtg ctggcactcg ccgatgcggc actcccggcg gactggcgtg
    17401 aaatccgccc cgccctgtac atgctggtca cgggcgggtg cgccgcgatc gcctacgccg
    17461 ccgccttcct tttccttccg ctcgccacac tcgcggacga gagcctgcgc tggcgccgta
    17521 cgctccggct cgcccgctga cgaaccatga agcacctcga cctcctggag cacttttcgg
    17581 ccgccgacgg cggcatcgcc atgcagttgc ggggcgcgca gcggcgcctc gggtcgcatg
    17641 acggcgtcga catcgtgcgc gacgatttcg ccgacgaggc cgcgagcgcg ctcttcctgc
    17701 gcgtccagcg gaccgccacc gcggaatcgg tccgcctgcg gctcgccggc aaccacttgt
    17761 tgaaacgctg cgccatcggc ggctggatgt tcgagccgac gacacctggc gaggccgtct
    17821 tctgggtgcc gcgcctgccg gtatgccgga ccctggatgg cgtcggccgc atccgcgcgg
    17881 aatccccggc gacgctggcg aacatggaga tcggcggcgg cgaggtggcg gccacgttcg
    17941 agctcgcacc cgaccaggtg ctcgattgtg tcgtctggcg gctggagtcc gaggcgtcgg
    18001 gcacggacgg gagccgcgcc gcagacgagc tgatgacggc aagcgcactc gagtcccaag
    18061 ctttcttcgc atacgcctcc cataccgcga cgcgatgcgc ggcggatttc tacacgcaca
    18121 tcgtgcacgg ccaggtctac ggcgcctgct gggcctggcc caagaagctg aagatctgcg
    18181 acgagctcga cgcgctcgcc ctgcacatgg tcgccagcgc gctcggccac agcaccggca
    18241 agcgcgtcta ccgcctgttg cgccgccaga tcgtgctggc cgtcctctgc cgccaggacg
    18301 ccgacggcgg tttccggcac ggcgaatgga ccgacgagta cgagagccac aaccgcctca
    18361 tcaatggcgc gatgcagttg ctggccaccg agttcgccgc cgcgcccgag ccggccctgg
    18421 agggcgcgct tcgccggatc gcgcgctatc tcgccgagca ggtcgaccac accgatgccg
    18481 gggcctggtt cctgcacgat tcgctggagc gctccgagga aggcatgcgc cactacccgc
    18541 tggcgtggga acgcggacgc tggctgggca agtcgccgac gaacctcctc atcctcaaca
    18601 cccacctcga ctgcatgctg gcactcgccc gcgagcgcgc ggtgtgcggc gacgacaccc
    18661 acgacggact gctgcgctcc gccgaggcct gcctgcgcac ggtgatgtcc gcgcgtccgg
    18721 ccgactggct gttccgcccg ctgctcgcgg tgatcgccct ctccttgctg ccgaaggcgg
    18781 agcaggaaca actgccgttc gcgcgccgcg ccttgaagcg gctcggctgg aaatacctga
    18841 ttccgcgctg gcacctgatc cgccgacgct ttccacgcct gctgatgccc gccggctaca
    18901 tcgaacgcag cctcggccaa gccgatttcg tgcaccgcta ccacggggtg cacctgatgg
    18961 atttcgaacg cctgctcgcg tgcggcgccg tcgacccgaa agaccctcgg tggacgtcca
    19021 tcagcgacgg cctggtcgac ttcggcgtaa atagccgcgt cacccgactc tggaaggaga
    19081 ccgcggccag ccgggactcg ctcgcattct gggccgaggg cttgtaccgc cgctgcctgc
    19141 gcacgcgcgc gcagcgcgac cgcgagctgc tggcgaccgc ggtgctcgac ctgcacgatg
    19201 ccggtctcgg cctgccgccc tcgctgctcg gcgccaacgg cgagatcgtg cccgcacaat
    19261 cggccgcgcc cacgccgtct gccgcggatg cacgcctcag ggtgatcaac ctgggcgcgc
    19321 gcaagacccc ctgcctcctc gtggtcaaca ccgccacgac ggacctgccg cttgccttcg
    19381 agccgccgct cacagccgcc caccccgggt ggatggacgc tacgcggagc gtgccggcgc
    19441 gcggctggat cctgttgcag cccggcccgt ccgacgcaga gcccggcccg gcctcctcct
    19501 ctcacgcatt cctgcctgca cggccccggt aacgatgagc aacgcccgcg cgatcgcctt
    19561 ccacctgccc caataccacc ccacccccga gaacgacgag tggtggggca agggcttcac
    19621 cgagtggacc aacaccaccc gggcgaagcc cctctacccg ggccactatc agccgcacct
    19681 gccggccgac ctgggctact acgacctgcg cctgcccgag gcccgccacg cgcaggccga
    19741 gcttgcccgc gcatacggca tcgagggctt ctgctactac cactactggt tcggcggacg
    19801 gcgcatcctc gagcgcccgg tcaacgagat cctcgccagc ggcgagcccg acctgccgtt
    19861 ctgcctgtgc tgggccaacc acagctggag caacatctgg cagggcgtgg ccgatcgcat
    19921 gctgatcgag cagacctacc cgggcatgga cgaccaccgc cgccacttcg aatggctgct
    19981 cgccgcattc cacgatccgc gctacatccg cgtcgacggc aagccgatgt tcctgatcta
    20041 caacccgccc gacattcccg acgtggcgcg ggtgatggac tactggcgcg agctcgctgc
    20101 gcaggcgggg ctgccggggc tgcacctggt cgcggtgaat tacctcggcg ctgcggtcga
    20161 tccggccgac ttcggcatgg acgccgccac ctggcagccc ctgccgccca agagcggcca
    20221 catcccgtgg cgctacccgg ccttgaaggc gcgcatgcgc atggcgaagg gaaaatacaa
    20281 gctgaccgtg ctggactacg cccgcatcat gagcggtctg acccgcgcca gcccgccgca
    20341 attcaccgag taccccacgg tgctcccgaa ctgggacaac accccgcgct cgggtctcaa
    20401 cggactggtg ctgcacggct cgaccccgga gctcttcaag accgtgctgc gccgcggcgt
    20461 cgacctggtg cagggctacc cggccgagca gcgcatcgtc ttcatcaagg cctggaacga
    20521 gtgggccgag ggcaactacc tcgaacccga ccagcgcttc ggccacggct acctgcgcgc
    20581 ggtgcgcgag gtcctgcagg agacgcgctg aagtgtgcgg catcaacggg atcttcgcct
    20641 acgcccccgc tgctaacgca gtggaccgcg ccgaactgct gcgggtgcgc gacaccatga
    20701 tcgagcgcgg ccccgacggt gccggcgtgt ggaccggcga cgacgggcgg atcggcctcg
    20761 gccaccggcg cctcgccatc ctcgatctca gcgcggcggg cgcccagccg atgcactcgg
    20821 cggacggcac gctggtggtc agcttcaacg gcgagatcta caactacccc gagctgcgcg
    20881 gcgatctcga gcgcgccggc gtcgagttcc gctcccattc cgacaccgag gtgctgctgc
    20941 acctgtatcg ccgtcacggt cccgcgatgg tgggccgcct gcgcggcatg ttcgccttcg
    21001 cgctgtggga cgcgcgggcg cgcagcctgc tgctcgcgcg cgacccccac ggcatcaagc
    21061 cgctgtatta cgcggacgac ggcaggaccc tgcgcttcgc ctcccaggtc tccgccctgc
    21121 tggccggcga aggcgtcgac acgcgccccg aacccgcggg agcggtcgga ttcctgctgt
    21181 ggggctcggt tccggaaccg ttcacgctct accgcggcat ccgcctgctg ccggcaggct
    21241 cgaccctgct ggtgaacgaa gcggccggcg ccggcgcggc ggtacgctac tggagcctgt
    21301 ccgccgccct gcagcgctcg gtgcaggccg cggcggccgt cccggccggc ggggaggccg
    21361 ccttcatgcg cgaacgcctg ctcgagagcg tgcgcgccca cctgctctcc gacgttccgg
    21421 tcggtgcgtt cctgtccgca gggctggatt cgtccaccct ggtcggcctc gcccgcgaag
    21481 cctcgcccgc ccgcctgcgc acgctcaccc tgacgttcga cgacctgcag ggcacggcgc
    21541 acgacgaatg tccggccgcg agcctgatcg cccgccatct ggacgtcgac cacacctgca
    21601 tcgtgctctc tgcgggcgaa caggaagacg agctcgatcg cttcttcgcc gcgatggacc
    21661 aaccgaccat cgacggcatc aacacctggt tcgtgagccg cgcagcacaa caggccggcc
    21721 tcaaggtcgc actctcgggc ttgggagggg acgaattgct cggcggctac gccaccttcg
    21781 cagagcatcc gcggctgcgc gcgcgcgccc acgccagccc ctggcggcac gcaccccggc
    21841 tctaccgcgg cgcccaccgc ctgctcggcg cccgcctcgg ctggccgccc aacgcggccg
    21901 ggatcccggc ttgcggcgcc gatccggagg gcgcgtacca cctgctcaag ggcgtgttca
    21961 tgccctggga actggacggc gtggtcgatg ccgacgtcct ctgcgaaggc ctgcaagccc
    22021 tcgccgaaca cgatgcggct cacaccgagg cgcttgcggg gctgaacgac ttcggccagg
    22081 tcgcctgcct ggagtcggtg cgctacatgc gcaaccagct gctgcgcgac accgactggg
    22141 tcagcatgtc gcactcgctg gagatccggg tgccgctggt ggaccacctg ctcagcgagg
    22201 ccctgctcgg tctcgccgcg agcggacgcc tcggcccggg caagagcatg ctgccgcgga
    22261 cgctggcacg cggcctgccc gacgagatcc tgaaccgccc caagagtggc ttcgtcgttc
    22321 ccacctggcg ctggctgcgc caccatccgg ggctcgatgc gtggaagtcc ttgcccgcgc
    22381 tgcgccaccc gcgcatgtcc gatggccggc gctgggccta cacgctgctg agccgccacc
    22441 cggcaggcaa ggccctgatc cgcaactgaa ccagctccgc ccgaccccgc ccttcatcgt
    22501 ccgccccatg gaaaccaagc ccgatccgat cccgcccgcc accagcgcac gcatcagcgt
    22561 ggtgatgccc tgctacaacg ccgcgccttt cgtgcgcgag gcggtggaat gcgtgatggg
    22621 gcagacccgt ggcgacgtcg agctcatcgt ggtcgacgac ggctcgaccg acgcgagccg
    22681 cgacctcctg cacgcgctgg cggcggcgca cgggccgcgc atgcgggtgc tgcaccagga
    22741 ccgcaagggc ccttaccccg cgcgcaacct cggcctgcgc cacgcgagcg gcggcctggt
    22801 cgcctttctc gacgccgacg actacctgag ccccgactgc ctggacaagc tggccaacgc
    22861 cctcgaacgc acggatgcgg acatcgccta ctgcggctgg cagaacgtcg gcgagggtgc
    22921 gcccggcacc acgccctacg tgccgcccga ctatgcggcg ctcgacaccg cggccgagtt
    22981 cctgcgcgcc tgcccctggc cgatccacgc cgccctcgtg cgtcgcgctg cgatcgacgc
    23041 cgtcggcggc ttctcggagc gctgcttctc ggcgatggac tacgatctgt ggctgcgcct
    23101 ctacgcccac acgcagaaga tcgtgcgcgt gcccgaggtg atggccttct accgctggca
    23161 cggcggcggg cagatctcga agacgcgctg gaagcaggtc ctcgacgccc tgcgcgtgcg
    23221 gcgcgacttc gtcgccgcgc acccggagcg cgtcgcccac ctgcccgccg cccgcctggc
    23281 cgagctgacc gacggctacc tgctgcgcga ggcctaccgc gcgtactggc ggcgcgagct
    23341 cgacgacgcc caggtgctgc tgcgcgcggc cctgcgcgaa ggcgcctggg gggtgcgcga
    23401 cctcaagtac atcctgccgg ccctgctgcc cgccccgctc ttccgccgcc tcgtcggact
    23461 cgccgggagc accggatgag ggcggcggcc cagccagccc gccagcatgg ggtctaccgc
    23521 aacacccgca cacccgctac cctgccgccc gaatcgctcc tctccgctcg aacacgatga
    23581 cgacctcccc cgcccaacgc gtcccggtcc tgatgtacca ccgcatcggc gccacacaca
    23641 acgactggga acgcaagtac tgcgtgagcc cggaagcctt tgcggcacac atggacacgc
    23701 tcgcgcgcgc cggctggaag gcggtttcca tcgacgcctt cttcgcctgg ctggacggcg
    23761 gagcgtccct gccggagggc gccttcctgc tcaccttcga cgacggcttc cgcggcgtgc
    23821 acgaccacgc gggcccggtg ctgcacaggc tcggctggcc ggcgacggtc ttcctggtca
    23881 gcgcgctcat cggcgagcgc gacgcatggt gcgagaagca caatccggac ggccacacct
    23941 atccgctgat ggatcgcgaa cagatcctcg cgctgcgcgc ccggggcttc agcttccact
    24001 cccacacccg cgaccacgcc gacctgccga cgctcgacga cgcggcgctc caggcccagc
    24061 tcgccggtgc gcgcgacgac ctcgaggccc tgctcggtgc gccggtcgac tacctcgcct
    24121 acccgtacgg gcgctacgac gaacgcgtcc tgcaccaggc gcggcaggcc ggctaccgcg
    24181 ccgcgttctc ggtgcagccc gggttcaacc gtcccgaggt cgaccgcttc cgcctgcgcc
    24241 ggctcgacgt gttcggcacc gacaccccgg cgatgctgcg ccgcaagatc accctgggca
    24301 gcaacgacgg ccgcctgacg gccgcgctgc gctacaacgc cggccgggtc ctcgcccggc
    24361 tcggcattcg tcactgaggc gggatcatgg acggctccac gcccttcatc acggtcgcac
    24421 tgtgcaccca caaccacgcc gaccgcctcg cacgcacgct cgagacgctc gccgacctgc
    24481 atcctcccgg acggccgtgg gagctgctcg tgatcgacaa cggctgcacg gacgccacgc
    24541 ccacgctgct cgcgcgcacc gcatggcacc ccgcgggggt tgcggtgcgg gtggtgcggg
    24601 aagaacgcct cggcttgtcg aacgcgcgca accgcgcgct gcgcgaggca cgcggcgagt
    24661 acctgctgtt catggacgac gacgagacgc cagaccccga gtggctgcgc gcctatgaac
    24721 aggcgatgga agcgttcgag cccgacgcgc tgggcggtcg aatcgaggtc ttcttcgagg
    24781 acggcgtccg gccgcgctgg ctgcaggacg agctgctcgg cttcctcggc aagctcgacc
    24841 acggcgaggc acgctggctg gatgcgccgg ggacgccgat cttcggcggc aacttcgcct
    24901 tccgccgcgg cgtcttcgac accatcggcg atttcgacgc ccgcctcgga cgcatgggca
    24961 aggccaacat cggcggcgag gataccgaga tctaccgtcg cctgctggcg cacggctgcc
    25021 gggtgcgttg ggttccgcag gcgatcatcc accaccgcat ccagaccccg aagctgcgcc
    25081 gcggctattt cctcgacctg cacttccgcc agggacgcac cgaagggtcg gcgaagcgcg
    25141 gcagcggccg acgcatcccg cccccctacc tgatcgcgca atggctgcgc gccctcggcc
    25201 gcgccgccgg actcaggcta cgcacaggcg ccgaccattc gctgcggatg gagatgaacg
    25261 cggcctactt ctgcggtttc ctgctcggct ggatgcgcga ccgcggctaa gccgccgcag
    25321 cgcccccctg tctctctgat tggacctgcc ccgatgtcat cgttccggct gtcgcacctg
    25381 cacaagctgc tcgaccccga gatccgcgcc aagaacctcc agtggctgcg ctggcgggcc
    25441 cgggagctct actggtcggc cgcccatccg gccatgcccg acccggtctt cgtgatcggc
    25501 tgctcgcgct cgggcaccac cgtcaccttc gagacgctcg ccgcgtccgg gcagtacctg
    25561 catttcgact acgagctgcc gcagctctgg gacgggctgt acgggccgct gaacaacggc
    25621 tgggcatcgg aagccgcagg cccggaacat gccgaccacg cgcaccgcga acgcgcgctc
    25681 gcctacttct atgcgcgcct cggcgccggc ccggtgctcg acaagacctg catcaacacg
    25741 ctgcgcgtgg cctacctgca caagctgttc ccgcgcgcga agttcgtctt catccaccgc
    25801 gatggacgcg acaacatcag ctcgatgatc gacggctggc ggctgggccg taccgatggc
    25861 ggcttcggcc tggagaaatt cttcggcccg tcgccggtgc cggtggagat caacggcggc
    25921 gagttccgcg aatggcactt cttcctgccg ccggggtggc gcgactacaa ccgggccagc
    25981 ctggaagagg tatgcgcgtt ccagtggatc agcgccaacc ggctcgccct cgacgcggcg
    26041 cgcgacgtgc cggcggcgca gtggatccag gtgcgctacg aagacatctt cgagcgcccg
    26101 gtggagatgt tccgcaccgt gttcgaacgc ctcggcctgc ccttcgacgc gcgcatggag
    26161 gcccactgcg caggactctc ggggcgcccg accagcatcg tcaagggcac gccgaagaag
    26221 cagaaatgga aggagagcaa ccccgaggcg atcgcgcgca tcctgccggc gatcgctccg
    26281 ctgatgcgcg agctgggcta tgcgctcgac gactgagccg acggtcagcg tcgtcatccc
    26341 ggccttcaac gccgcctggt gcatccggcg cgcggtcgac agcgtgctgg cgcaggactt
    26401 caccgatttc gagctgatcg tcgtggacga cggcagcacc gacgacaccg cggaggtcct
    26461 gcgcggctac ggcgacgtgc tgcgcctcgt ctcccagccc aacggcggca tgagccgggc
    26521 gcgcaacgcc ggcatccgcc tcgcacgcgg gcgctacatc gccttcctcg acgccgacga
    26581 ccgctggctc cccgccaagc tcgggcgcca ggtcgcactg ctcgatcagc gcccggagct
    26641 ggccttctgc gccgcgacgg cgagcctcga ggacccggac ggcaagccag tcggcatctg
    26701 ggcctgccag ggcgacggca ccgcctcggt ggccgaggta ttcgccgccc atgcgctgat
    26761 cgcggggggc gcctcgtcgg tgctcgcgcg acgcgagccg gtgcaggcgc tcggcggttt
    26821 cgacgaggcc ctgttcggcg cggaagatac cgacctgtgg atccgtctgg ccgcatgcgg
    26881 cggcttcgca tgcatccccg aggcgctggt ggtggtactc aagcgccccg gcagcgtgag
    26941 ccggaaccgc gcgcggatgc gcgccggcgc gctggcgatg acgcgcaaga accgtcacct
    27001 gctgcccgcc gacaagcagg gcgcctactg gcgcgcactg tacgcgggca cgctgtgcga
    27061 ctacgccaag tgggcctgcc gcgagggcga tcgcggcgct gcgctcgccg atctcgcgcg
    27121 cgccttcgcg tgcgcgccca tcgggcgggg acgactgatc gcctcgatcg cgctcgccgt
    27181 gctcgccgga ggacggctat gaacgtcgcc accgccgccc gcagcctcac catcctgatc
    27241 tgcacgcaca accgcgcgga cctgctcgca cgcgtcatcg attcgctcga gtccgcccgc
    27301 cagcctgccg gctggagtgt ccgcctgttc gtcgtggcca acgcctgcac cgacggcacg
    27361 cacgaattcc tggccgggcg cagcgaccgc gcggatcggc tggcgctgtc gtggatcgcg
    27421 gagcccacgc cgggaaagtc gcatgcgctc aaccgcgccc tgcccttgct ggaggacgag
    27481 ctggtcgcct tcgtcgacga cgaccaccgc gtggacgccg actacctgct cggggtcacc
    27541 gccgccgcgg agcgctggcc cgaggccgat ctcttctgcg ggcggatcgt gcccgactgg
    27601 gacggcagcg agccggcctg ggtgcacgac gaaggcccct atcgcatcta cccgctgccg
    27661 gtcccgcgct acgaccaggg catggaggac ttccccgtcg acctcgaggg gccgatcccg
    27721 ggaggcggca atctcgtcgc ccgcctgccg gtgatcggtg cgacgggacc gttcgcgatc
    27781 gaactcgggc cgaccggaca cgatctcggc ggatcggagg acgccgactg gatcctgcgc
    27841 gccttgcgca agggggcccg cctgcattac gcaccgcaga tcctccagta ccactatgtc
    27901 gatagcgaac gcctgacctt gtcctacgtc gcacgcaagg gctaccagcg ctcgcaatcg
    27961 gtcacgcggg tgcgcgcgga gttcgaccgg gtgccgcgct acatgtggcg caaggccgcg
    28021 ggctacgccc tcgggctggc gttctcgtgg cggctgcagg cacggcgctt ctatctggtc
    28081 cgcctggctt cctcgatggg cgagatctcc ggcatccgcg accgccaccg gcgcaagcgc
    28141 aggcgtgcgg cactgccgat gctcggggcg gaagcgggat cggcggcctt gctggtggcc
    28201 gccgcgctca cgctcgcgct ggcggcggtg ctcgcgcgcc actggctggg cgaagccctg
    28261 ctcggcgccg gcgtggtggg cgcgctgacg agcgcggtgc tggtagccaa gtcggtgcgg
    28321 gacttctcgc gcaccgggcc gggcctgcgc gaagagatcc tcgcgcgcta tcgcggctac
    28381 gtcgtcttcg ccatcgcccg cctggggctc gcggccctcg gcctcgccgc gttctgggcc
    28441 ttcccgggca ccgcgctgtg gatcactgcg gcggaagcgc tcggtcggga accgccgctg
    28501 tggacgactg ccgcgggcgg cgccctcacc ctcgcgctcg cgacggtgta tgccggctgc
    28561 cgagcacttt cgcaaaaccc gggcctggtg atcgcgtcct ggcaatatcg taccgtccgc
    28621 atccatcgcc tgtggcgcgc gctgtcgcag cgcggtctcg acctcatcgc gcgcatcgtg
    28681 ctggcgaccg gaatcggcct ggtcggggcg atcgcgttgc tgcgctacca ccagggcggc
    28741 agcgcggacg cgggggccat gctgctcgtt acctgcggct acatcgcgct gctcgcctgg
    28801 gcgatctggg agcccgacgg cacgcacgcc cccaccccgc gacggcgcgc gcggcgcaac
    28861 gtgctgatga tcggcagcga tacgctccgg gccgaccgca tcggcgcgca acgcgaaggc
    28921 gcgtcgatca ccccgaacat cgacgcgctg gccgcacgag gcacccgctt cggcgcctgc
    28981 tacgtcccct gcgcgcgcac cgcaccgagc ctgatctcgc tgttcaccgc gacctggccg
    29041 caccaccacg gggtgcgcga caactacgtg gccggcgccg agacgcggct cgaagacaag
    29101 accctgccga acatactgcg cgcactgggc tatcgcaccg cggcggtttc cgactggtgc
    29161 ggcgccgatc tgggcaagtt cgatttcggc ttcgacatca ccgatctgcc cgaggatcag
    29221 tggaacctga agtacctcat ccgccagggc ccgaaggaca tccgcctgtt cctgtcgctg
    29281 ttcctgcaca atcgtctcgg ccgtcacctt cttcccgaga ttcactacct cggcggcgtg
    29341 ccccagaccg gcatgctcgg acgccgcgcg cgacgcacgc tgtcgcgcct ggccgcgggt
    29401 gacgaaccct tcctgctcaa cctcttctat tcgaccacgc atccgccttt cgcatcggag
    29461 cacccctact acacccggtt ctccgatcct gcctattccg gcgcgtccaa gttcgccatg
    29521 gcccggctga ccgaaccgtt cgagatcatc cgccgccagg gcgagccgcg cgaggagttc
    29581 gacctcgacc agatcctcga tctctacgat ggctgcgtgg cccagttcga cgacgaggtc
    29641 gggcgcctgc tgcgccagct cgacgacagc ggcctcgcgg aggacaccat cgtggtgctc
    29701 tacagcgacc acggcatgga gttcttcgag cacggcacct ggggccaggg caactccgcc
    29761 ctgggcgact tcagcgcgcg cgtgccgctg atcgtcgtcg acccggcccg accgggcggc
    29821 cagcgcgtcg accaggtggt gcgcagcgtg gacatcatgc cgaccctgct cgatctgctc
    29881 ggtgcgccct cggtcggctg cgacggggtg tcgctgcgcc cggcgatcgc cgatccggcg
    29941 accgacctgc acctgcgcgc gttcaacgag accgggatct ggatcgcacc ggtccccggt
    30001 ctgcccgaag gacacctgag ctatcccaac ctgctggaac tgctcgacgt tccggacatc
    30061 gcggcgggca gcctgtcgct gcgcgagcgc taccgccaga cggtactggt cgccaaggac
    30121 cgcatggtgc gggacgggcg ctggaagctc gtctaccagc cgctcgagca cggcaggctt
    30181 ctcagcctgt acgacgtcga gagcgacccc gggtgcaccg cggacgtcgc gtcccggcat
    30241 cccgcagagg tcgagcgcct gtgggcgcag ctgcgcgcct ggatggcgaa cgacccggcg
    30301 ctgcgcggag atccccgcct ggacctgccg ccgacgcccg cagcgacctc agccgcccgc
    30361 gcgccggaag cggatctcgc gcccgagatg cggtagcggg atcacgatct ccgcatcggc
    30421 tgcgggaaca tgatcgcggt agcgatggag gacgatccgc tccggtgcct cggccccgcc
    30481 ggcggcatgc acgggaaact cggccacgcg ttcgaaatcg gcttgcgcga gcagctcggc
    30541 aagcatctcg aactcgggaa cgccgacgat gtccgggcgc tcgatcacca cgtattcgat
    30601 tccgtagcgt cgaatcaagg cgcggatgtc gtcgatgccg gagacgtgcg agctcatgcc
    30661 gaagtacttg tgtaccgcga gcgagaccag gaccttgtcc gcgcgcaaca ccacggcctc
    30721 gcgcgcatgg tcgagctgcc gcagatggaa gatgaagctg ccgtcatgct tgccggcaaa
    30781 cagcacggga gcgcctgtcc gccgctcgtg gaccgcctgc gccacctcgc gatagccgtc
    30841 gatccgcggg accggctgcg tcagcgttcg ccagccctgg aaagcgagca tgaccacgag
    30901 caggacgagg aagccgtcgt gcgatcgccg cccccgcagc aggaacagcg gacgggcggc
    30961 cagcacggcg agcgcaggca gggcatatag cgtgtagcgc ggcgcattgc ccgcaccgcc
    31021 gagcaccagc gtcatcacca cgtaccagca cagcagccag gcgtggatca gggccagccg
    31081 gccctcgccc tggcgggccg gaagcagcgc ccccgcgagc gacagcagcg ccagcggcca
    31141 gccccagacc tcgatcaggg cccgcggata ggtcagccag cgttcgagcg agaggcgggc
    31201 cgcactttgt ccgacttcga cccggctgtc cccgacggtg gccgcgaggc cgagattgcc
    31261 gaacttgagc gcatgcagcg cgaccagggc gatcgccagc gccgcgatca gataggcggg
    31321 cacggcctgc acgcgccaca gggcggcacg ccgcgacgag atcaggccgt acgccagcag
    31381 cgccggcagc aggagcgcga cggtctgctt gaccgccagt gcggcggcga tcgccaaccc
    31441 ggccgcgatg gcccgcgacc atgccgggcg gtcgagatag cgctcgaagg tccacaccac
    31501 gaggacgagc aaggcgatcg ccggcgcctc gagcatgacg ctgcgcgccc aatggttcca
    31561 ctccgggacc gcgatgaaca gtgccgtcgc catgaaggcc atcagggggt tccagcccgt
    31621 gcgcacccag tcgaaccaca gcccggcggc gagcaggacg aaggcgaaca cggtccagtg
    31681 cgcaaccgtc tcgctgaccc cgaagagcgc gaagaaaccg gcctcgacga aatagaagcc
    31741 gggcaggtac cagttgagcg cgagcgcagg atactgcgcg aaatagcgca aggcgtagcc
    31801 catcgggtcg tcgagcggca ggtcgcgcag caggtcgagg atgaacaccc cgcccatcgc
    31861 atgccgcgtc tcgtcggtcc accagaatcc gctcccaccc agcgcgggcg cgagaaagag
    31921 cgcggagagg gcgaagagga gcgcacaggg aagccaggat ctcatggcgg ggagccctcg
    31981 ggagatggat cgggaacggg cagcggcccg agttgctcga gcacgaagcc ggtggtctgc
    32041 agcagcggcg cggcaccggc caggggcgga tcgccgaccc gccgcagatg gagcacgcga
    32101 tgcccgacca ggctggtgcg caaggccgcg gcatctccaa agccggtgca gaagcgtacc
    32161 ggctcgtggg cgcggtcgcg gcgaagctcg cagcgcccgg ggttggtggg gtcgcccgcc
    32221 agtgccatgt gcgcatcggc cggataggcc ggcccgtaga agcgcaccag gttgaggtat
    32281 tcctgtttga cggacggggc tgcgatcagg attcccggcc gcccctctcc gccgaacacc
    32341 tgcgcgctga tcgccgccat ctcccggggg acgtaatcga cgcgctcgta gcgcggcacg
    32401 aacatcgccg caccgaggcg ctggatccgc cagagacccg gttgctcgac gaaccagcca
    32461 tagatcgggg gtctcgacat caggacgaaa accaccgcca ggccgagggc cgcgcacctc
    32521 tgccgcgccc ccagcacaaa ggtccagccc gccgccgaca gcaggatcag gggagggacg
    32581 aggccgatca gcatgcgggt ctggacggcg gagtcggagg cgatcagcgc ccatgagggc
    32641 aggcccgcga gcagcagcca caggacgccg aaacgcacca cccgcgagcc gcggaaccgc
    32701 ggcagcgcga acacggcccc gagccagagc atgccgccgg cgacgaacac caggtttccc
    32761 gcatgcaccc cctcgcgcgc gcgctgtgca aggcttgcgg cgaaggccag atagctgcgc
    32821 gggtccgcca gcgggtggaa gacgaacgca taggtggcgt agccgagcag ggccgtcccc
    32881 cctgcgagga cgaagcggcg cagcgtccgt gcgcgtgcct ccgcggcgcc ggcaaaaggc
    32941 agataggcga gcatgaacgc cagccagatc cacacgctct ccacccgcac ggtgtagccg
    33001 aggaaggcca accctccgga gaccgccgcc agccagcccg cggccgtggc ggtcgacgcc
    33061 agcaaggccc acaccgccgc agcgccgagc ccgaagctga gcacctcgct caccaggttg
    33121 ccgctcaggt aaccatggac atcggacagc acgaacagaa gggcactcga cgccagccca
    33181 ccggccccga ccgcacccgg ctcgagtgct cgtccggcga gatggctcac ccggcaccag
    33241 gccagcaggc cgagcggcat ggcgaggacg aacatccagt gggcggccgt gatcgccgca
    33301 tccgtgcttc cgaacgcggc gaccggcgcg ccgagcagcg cgatgtggcc gagacggctc
    33361 gatcgccaga aagcctccga gtagctctcg tcctcgggcc aggcaccggc gagctgggcg
    33421 acgatctcct gcgcattgcg tgcgacatcc acgaggtagg cggtctcggc gaagcgcttg
    33481 gcctgataga ggtagatcgg cgcgtcccag tgcacgtccg cgagcggtgc gcgcgacagc
    33541 gcgacgagca gcatcgcgag cccgaccgct agccagagta cgcgggtcct gcccgccacc
    33601 cggccgcgcc ccccacccga cgcgcagccc ccgcccgcgc ccggcaggca agctcgatgg
    33661 tgactcatgc ggatgcgtcg tgcgggtggc aggcggcgct cagagcgcgg cgagcacttg
    33721 ctggatgatc tcggcctgcg cgtcctccat gcccagccac aggggcagcc gcaccagacg
    33781 gtcggcgatg ttctcggtga cgctcagttc gccgtgggca cgaccgaagc ggcggcccgc
    33841 cggcgcatcg tgcagcggga tgtagtggaa cacgcagtgg atcccgaggc cgcgcaggcg
    33901 ctcgatgaag gccgtgcgtt gctccagccc cggcagcagc aggtagtaca tgtgggcgtt
    33961 gtgcccgcaa tgtgcgggga tcaccgggcg ccgcagcctg ccctggcgct cggcctcggc
    34021 aaaccactgg tggtaggtgt tccagatgcc cagacggcga cgggtgatgc tgtcggcctc
    34081 ctccatctgc gcccacagga aggcggcgat gagttctccg ggcaggtagg acgagccgat
    34141 gtccacccag gtgtatttgt ccacctggcc acggaagaac tgggagcggt tcgtgccctt
    34201 ctcgcggatg atctccgcgc gctcggcgcg ccccggagca ttgaccagca gggcgccgcc
    34261 ctcgcccgag atgatgttct tggtctcgtg gaaggagagc gcccccatgt gcccgatcgc
    34321 gccgagcggc cggcccttgt agctcgccat caccgcctgc gcggcatcct cgacgacgag
    34381 caggtcgtgg cggcgggcga tgtccatgat cgtgtccatc tcgcaagcga cgcccgcgta
    34441 gtgcaccggc acgatcgcgc gcgtgcgcgc ggtgatcgcc gcctcgatca gacgctcgtc
    34501 gatgttgagc gtgtccgggc ggacgtcgac gaacaccggc accgcgcccc gcaacacgaa
    34561 ggcattcgcg gtggacacga aggtgtaggc cggcatgatg acctcgtcgc ccggctccag
    34621 gtcggcgagg atcgccgcca tctcgagcgc ggcggtgcac gaatgggtca gcaaggcctt
    34681 ggaggcaccg gtacgcgcct cgagccagcg cgagcactgg cgggtgaagt tcccgtcgcc
    34741 cgcaaggtgg ccgttggcgt gggcctgggc gatgtaccag agttccttgc ccgtcatgtg
    34801 gggcttgttg aaggggatgt tcatggctgc ggcccggtcg agtgcgagtg aggaacggaa
    34861 ttcggacgga agacccaaaa cttctgcaga agaaacagca ggatcgcgag cacgacgatc
    34921 gacacgccct ggacgagctg atggggatgg cccatgcggt cgaccagcac catcagcgcg
    34981 accaggttga gcaggtaacc cgctgcgtag gagagacagt agcgcacgaa cgccggcccg
    35041 tgcatcccgc cgtgacggaa ggaccagcgc ttgttgaaga cgaaggtctg caccaccccg
    35101 agcagataga gcacgctcat ggcgagcttg gccccgacgc ctgtcgccgt cagcgcgaga
    35161 taagccaggt agagcaccac gttcgatgcc agccccaccg tcgcgtagcg gacgaactgg
    35221 cccagggtgg cttgcgcgct catgcaagcc gaaccctgtg gaaagtcatc gagggcgccg
    35281 ggaagctgaa gccgagacgc gcatagaggt tgagcacccg atggttgcgc gccacgatgc
    35341 tggtacgcac ccgggtcgcc ccttcgaggc gcgcctggtt catcatcgcg cgccaggcgc
    35401 gcaagccgta gcccctgccc tgccgctgcg gcacgaccgc gttgagatgc cagtagcagg
    35461 tgccgtcggc aagctgctcg gtgacgaaga aggcgaccag ttcggcccct tcacgcacgg
    35521 catggaggcg ttgcgaggga tgctcgaggc tgctgcgcac ccagttctgg tagcgcaggt
    35581 cgccaagccg cgatggcagc cgcgggtcga cgtggaaacg ttcgttccgg aaggcgcctc
    35641 ccgccacctc gacgatttcc tccagatctt caggtcgcgc tcgttcgacc accagtccct
    35701 gtgcatcgcg cggcccggcc gactgcaggt cgccgaactc gggctggaag accatctcga
    35761 tgaaacgaaa gccgatctcc tcgagcagca tcgattcgcg cagccgatcg tgggggagcc
    35821 ggcagctcgc cagccccacg cccccgacct cgcatgcggc gacgaagggc gccagatccg
    35881 ccgcgctggc gcccgtgcgg gcttcgatgc gcgacacctg caccaccgga tggccgaata
    35941 ccgccgtgtc ccagggggcc tccgcccatt cgaggcggag ctcagccgtg tcgatgcggc
    36001 cgtgcagggg gtgggagggg gcggcttcag cagcactggt catggacagt ctccgtgtcg
    36061 ccctgcgcag cggtggagga tcgcagcgca tagggcggac gctccatcga gcggaaatgc
    36121 atcctcgcca ggtactcccc gaagatcccc agcgcgaaca gctggacgcc cgagaagatc
    36181 gcgatgatcg aggccaggaa ggggaatccg ggcaccgcgc ttccgtcgac gagatagcgc
    36241 aggagcacgt acaggagcac caggaagccc atcgacccga aagccagtcc gagcacgctg
    36301 gcgacctgca gcggcaggac cgagaagccg gtcatcatgt tgatcgcatg attgatgagc
    36361 ttgcgcaggg tgtagccgga atcgccgacg gcacgctcgt cctggcggac gagaacggag
    36421 ccgaaacgcg tcgtgcccca ggtgagcagc acgtcgatat tgaccgacgg gctgcggtag
    36481 gccgagaagg cctcgcgcag gcgggcgcgg aagacgcgga acgcgctcac cttgctcgcc
    36541 gactccacgc ccatcgcccc ctgcagcgcg agcttggtga tcctcgaggc ctggtcgcgc
    36601 aggaagccgt gcgtctcggc ctgtggcgaa ccatagacga cgtcgtagcc ttcatccagt
    36661 ctggccagca ggcgatggat ctcttccggc ggattctgca ggtcgtcgtc cagcgtcacg
    36721 acgagctcgc cgcgcgcggc ccgaatgccg cacagcaggg cgttgtgctg gccgtagttg
    36781 cgcgccaggc gcaggccgag cacgcgggga tcggccgcag ccagctgctc gatgacgctc
    36841 caggagtcgt cgccgccgca gtcttccacg aggatgatct cgaacggccg gccgatctgc
    36901 tcgagcgcgc cgacgagccg tcgatgcagt tcgggcagca tggcctgcga gcgatagacc
    36961 ggaacgatgg cggaaatatc gatggacatg gtcgtcttca gggggcggtc agggtttctg
    37021 cggctcgccg aacgactcgc gccagcgctt ctggatctcc gccgggggac agaaccggca
    37081 gccttgggaa gcgccctcgg cggtgccggc atcctggtcg acctgcgccg ccgaacgcgc
    37141 cgcggtcgcc gcctcctgcg tcactgtcgc ctcggtggtg gccggagcgg cgtctgcagg
    37201 ctccgcctgc gacggctcgt cgggcgctgc cggcttcggt gcaggcgggc gcaccagcgg
    37261 cggcagctcg acgccgaact cctcgcccat cttgcgcagc cgaaagtcgt tcggcgaaag
    37321 cttcagggcc gtgcgcacga cctcggccgc cttggcgcgc cgatcggtcg cccggtacag
    37381 ggtggccagc tgcgcggccg cccgtgcatg gcgggggtcc agttgcaggg cattgagcag
    37441 ggcggactcc gcctcgatgt gctgcttgcc gtagtactgg gcccagccgc gcaggaaata
    37501 gacctccggc atgaggaagt gcgaccgtgc cgcatggctg atgatgtagc tggtctcgtt
    37561 gacgatcgaa ccgacgtagc gctcgcgctc cttctgcgag gcggagccga ccaccttgtt
    37621 ctcccactgc atcccgaagc agaagtgatg gaagtggaag tatttctgcg gattgttggc
    37681 cgcctgccag gcggcattgt tctggttctg caggcgctgc acgcaccagg cgcgcagcgc
    37741 ctggtagtcg gcctccgttc cggcgctcgc gcccgtcatc gcgatcgacc cggcaagcaa
    37801 cacggctgct cgcagcactt taaccatcat cgtctcctgt catttcggcg cttcgggtcc
    37861 ttgcgcccgc gcttcgcccg tggcgacatc cgcctgcgca cgcaagcgcg acaggcgctc
    37921 gaatatcacc tgcgccgcaa gcgcatgccc tgcgccgctc cagtgcgtat cgtccggcat
    37981 atagacgtcg acgacaccgc gctccacggc gtcgcgaatg gcgaggtcga gccgggcgtc
    38041 gggcacatcg agtccgtcga agagctcgcc cagccgctcg gctggcagtc gggcgggatc
    38101 gcgcgcccag ggacgatacg cgctcgactt gtccggcgcc agcgcggtca tgaacaccgt
    38161 ccgtccgtcg gcctggaagc gcgtcgccag gtctgcgagg gcgcagcgga tccggcgcag
    38221 gtgttccgca ctccaggcgg ccttgttgaa atcgtcgccg tacaccagca ggcgggcgga
    38281 tgcgcgcgac gagaagaggt cggcacgcac gagttcgacc tccaccgtgt tgccggcctg
    38341 cacaccgaag agaccacgca tggcaccgcg gtagagccag ctgcgcgcga acccggggtt
    38401 ggcctccgcc agcgcatgcg gccgcggcac cgcgcgggca tgcgccacga ccgccatcgg
    38461 cggcggcagc gcgagcggcg gcgcggggcg cgagcccgga cacagcccct ccaccttcgc
    38521 gtgatccgca gccaggccgc gctcgaccac gttccagatc accaccttcg gcggatgttc
    38581 cacgaacagg ggcgacgcgc gcaaggcgtc cacgtctacg cgatggacgt cgagcgtatg
    38641 cacgctccag ccggtcattg cggcgaggtg gttctgccat tgctgggccg gtcgcaggtt
    38701 cgcgaacgaa tcgccgacca ccaggatgtc caccggccgc gtccattcgc cgaacgaggt
    38761 cggaagcgga tcgaattcga gcaccggctc gttccagcca tgcgtgcgct cggcgtaccc
    38821 gccgaggcgg gtcaggtcgc catagaccgg ctgcaaccac cacgccagca tccacgcggc
    38881 cgcgaccagg accagggtga gtccgacgac gaagagggaa aaggcgcgcg gctgcatcag
    38941 aactggaagt acaggaactc ggaaatcgca tcgccccgcg tcagcgccgt ggcgagcagc
    39001 agcacggtcg cgaccagcca gggggtgccg atgcggaaac gcagcagccc cgcttcatcg
    39061 acgccgccgc ggaagtcgag cgcgggccgg tagcgggaca tgatctgctg cgtgttgggc
    39121 gcgaagaagg cgatcgccgc caggatggcg aggtcccgcc agacccaggc atcggcgaac
    39181 tgcccggcca gctgtcccgc gcccaccccc atctcgccga cgaggcggtt cagccgccag
    39241 tgcagcgcgt tgagccagtc gggcggcacg gccacgccat tcgcgcctgc catcgcgccg
    39301 agcatggcga gcgcggcatc gaaggagttc gcgcggaaga acacccacgc gacgaccacc
    39361 gccacgaagg tgagcgccca gccgagcacc cgccacgccc ggccgccgcg caggccgagg
    39421 cggcccacca gccagcgcca ggcatgattc accatcaggt agaacccgtg cagcgccccc
    39481 cagaccacga aggtccagcc cgcgccgtgc cagagcccgc ccagcaccat cgttacgaac
    39541 aggttggcgt ggcgccgcaa cggccccttg cggttgccgc ccagcgcgat gtacaggtag
    39601 tcgcgcagga aacgggacaa ggtcatatgc cagcgccgcc agaactcgga gatgctgccc
    39661 gccttgtagg gcgaatcgaa attgagcggc agcttgacgc cgaacatgcg ggacaggccg
    39721 atcgccatgt cggaatagcc ggagaaatcg aagtagagct ggaaggtgta ggccagcgcg
    39781 ccgccccagg cgtcgagcag gctgagcgcc tgcccggcct cggcggcctg gaacacggcg
    39841 ttggcatgcg gcgcgatgcc gtcggcgagg accaccttct tgaagatgcc gagcccgaag
    39901 atcgccagcc cgaccgcgat gttctcccag tcgagccggt aggtggatgc gtgcgcgaac
    39961 tgcggcatca tctcgcggtg atgcagcacc ggcccggcga tcaggtgggg gaaataggtc
    40021 acgaacaagg tgtagtggac gaagttgaac tccctcgcct cgcctcgcca ggcatcgacc
    40081 aggaaggcga tctgggtgaa ggtgaagaag gagatcccca gcggcagcac gatggcgtcg
    40141 aaatgccagt ggccgtcgaa gacgacgcgg gcgctgtcgg caaagaagtt gaagtacttg
    40201 aagtagccga gcaggagcag gttggcgccc accgcgaaga cgaggagcgc acgccgacgc
    40261 tggccggccg gacgtgcgga ctcgcgcgcc aggcggacgc ccatgccgta gttgaagacg
    40321 atcgacgcac cgagcaggcc gaggtagatg gggttccacc aggcgtagaa gaagcacgac
    40381 gccgccgcca gccatgccgc ggcgaggcgc tggctcgtgg cggcgagcca gaagaagccg
    40441 accagcacga tcggcaggta caggtagatg aactgcggcg aattgaacag catcgctcag
    40501 gcgcgcaggc agttgcgggt gcgctcggcc accgcctgcc agctcagcga ctggcgcacg
    40561 cgcgcgagcc ccgcctgtcc catcgactcg agcagcgggg ggtcgtccag caggcgcgcg
    40621 agggcgtcca ccagttcggc ccccgtgctc gcgtcgacgc gcaggccggt cacgccgtcg
    40681 agcaccgccg agccggtgcc gccggcacgt ccggcgatcg ccgccttgcc gcaggccgcc
    40741 gcctcgagga acaccatgcc gaagccttcg gtgtcgccgc cgatctcgcg attggccagc
    40801 acgaacacat cgcaggcgtt gtaccagcgc ggaaggtcct ccatcggcac gtggccgagc
    40861 cgatgcaccc gctccgcgac ccccgcctcc cgcgccacgc cgtcgaggta gtcggcgtcc
    40921 tcgccgatgc cgacgatcgc cagatgcacg tcgacgccgc gcgcgcgcag cgccggcagg
    40981 ctgcggatca gggtgtcgaa acccttgcgc cggctcaagc gtccgacgga caggatcagc
    41041 cgtgccgaat cgcccagccc gaggcgcgcg cgcaggtcgc ccgcgggcag cccggggcgg
    41101 aagcgctcgt tgtcgacacc cggatggatg agcacgatgc gctcgggccg gacccccatc
    41161 ccgatcaggg tgtcgcgggt gaaatcgctg ttggcgatga cccggtccgc gtggcgcaag
    41221 gcgaagcaca tcgccttgta cttgttgccg cggccccagc cggtgagctc ttcgccatgg
    41281 gcgtagatca cgaccgggcg caaggtcagg cgggcgacga gccaagccac cagcccctcg
    41341 ggcagggcac gcccggcgtg gatcgcatcg aatcgccgcg tgagcgcgag ccgcagcgcg
    41401 cagacgagga acctgaggta catgccgagc gactccggcc gcagccagcc gacgcgctgc
    41461 aggttcaggc ggtgcacgct gttggggtga tcgcggtcgt actcggcggc gcccggaacc
    41521 tgcgccgtca ggatgtggat ctccttcccg ccgagtcggc gatagacctc gtcgaaccag
    41581 acggcggtgc cacccttggt gggcaggaaa agctcggtga ggaccaggta gcggttccgc
    41641 atgggagatc tcgggatcga agcgcgccag atcgtatcac cgtgcccgcg cggcgcagcg
    41701 gccatgcatc acgcccgcga tcaggaagac cggcgcgagc gcgaaccgca ggccgctgta
    41761 tttcaggaag cgccgcagcg cgggctcgtg ctcgacccgg gccaagaaga cggccaccct
    41821 gtgcggcaag gaacgggtga cgagccccgt ggccgcagcg gccagttcgt cggcgagctc
    41881 ggcggtggac aggaagcgca cgtccggatg gcgttcgagc accccgcgca aggcccgctc
    41941 cagctcgtcg ttcgccgcgc gcgcgaccgc tgcggcgccc acgaagctct cgcgatgggt
    42001 ctcgagcagg accgggcggc cgaggcgccg cttcgccgcc acggcctgcc agacctgttc
    42061 cgcccgatgg ccgcggatcg gttcgaaata gtcgtcgcgc accaactgca tcagtccgtc
    42121 ggcggtgcgc tctccattgc ggatgacgcg cgtcggcggc atcagtgccc cctggccgtc
    42181 acgcccctcg atgcgacgcc ccggggtgac gatgcagcgc acgctgctcc ccgcccacgc
    42241 acgctcgacc gcgtcgtccc agacgaaggt gttcggcacc gccaccaccg gcgccgcacc
    42301 gaacaggcgc tcgagcagcc gcgcctcctc atcgacggca cattgcaccg ccgcgggatc
    42361 caggggccgg ctgggcagca ccgaggcgtc gatccagcgg ctctgcaaat gcgacggcag
    42421 cccttcgctg cgcgcctgcg gatcggccag ccagtcgcgc agcgaggcat caacgcgggc
    42481 tcgcgcaagc agcgcatcgg gccagaaatg ttccatgccg tgtcgctgga gggcgaagac
    42541 ccccgcgccg cagccggcac gcatcgcctc gaccatcggc ccgaactcgg gcgcatcgag
    42601 cgccctgcgg tggtagcgcg caaggccgtc ggccagcatg gcggccccgt ccggcaggct
    42661 gcacacgaca ccgagggtca tcaccgccgg cctcccgcgc tggtcgcgca ccgcggcgag
    42721 ccgggaagcg agccctgcaa gcgcgtcggc gtccgccacc ggcccggggc cccagtcgtc
    42781 gctctcgacg atcagcacgg gctgtgcgag cacgggttcg ttccacaggg ctcgcagcgc
    42841 ggcacggtgc acaacgagga atgccagcac cgccgctccg ccccacagca cgagagcaag
    42901 aattgaaaac gacatcgtaa agacgctcta cctcgcatgg atcgttgggg cggacccgcc
    42961 agccagcaca ggccgtcgcg aacctgaatg caacggccgt acgcgtttcc ccgcccacct
    43021 gtgtactgca ccgcacttgc gcgaagccta ctcggctacc atagaccgcg atgtgatcaa
    43081 ttttactatt caagacgatg acgacacgac gagtactgtg cggctggatc aacctggacg
    43141 aacgcgtcgc aagccaggac gcactcgctg cgatgctcgc cgccgagggg ctcgcaccgg
    43201 cacagatccg cgtgcagacg tccgaggcag gcgttttggc ctgcacccat cccgcgggga
    43261 gcagtgcggt cgacctgcgc tcgggcagca tggccgtgct gctcgggaac ccgcgctggc
    43321 ccgacgcgcg gcttgccacg ctggctcata gcgagggcca cgcagccgcg ctgctcgatg
    43381 cctggcggaa cagcggtgaa gctgccctgg aaacgatcca gggcagcttc gccctggtcc
    43441 tcgtcgacag cgctcgcgac cggatcctgc tcgcggtcga ccgctgcgga gcggagcgcc
    43501 tgtgcttcgc cgccgtgggc ggcacgctgg tcttttccac cagcgcgcga gcagtggcgc
    43561 gccacccgct cgtggcagcg acgatcgaca ggcaggcgct ttacgaatac ctctattgcc
    43621 acatggttcc aagccccgat acggcgttcg ccggcgtcga gaagctgctc cccgcccaat
    43681 gcgcgagctt ctccggcggc cgcctcgagc gccgcttcta ctggcagctt ccttacgcgc
    43741 cggaagtcga cgccgacgag gccagcctgc gcaagcgatt cctcgacctc accgagcagg
    43801 cggtcgcccg tcagtacgac gagaagcgat cgaccggcgc cttcctgtcc ggcggcacgg
    43861 acagttcgac ggtaagcggg atgctcggga aggtcagcgg gcagccggcg cgcacctact
    43921 cgatcggctt tcgtgcggaa ggcttcgacg agatcgaata tgcccgcatc acctcgcgcc
    43981 acttcggcac cgaggcgcac gagtattacg tgaccgcaga cgacatcgtc gaagccatgc
    44041 cgctgattgc cgcggcctac gatgaaccgt tcggcaatgc ctcggccgca ccgacctatt
    44101 tctgcgcgaa gatggcacgc gagaccggta tcgaaatcgt gcttgcgggc gacggcggcg
    44161 acgagttctt cggtggcaac gagcgctatg ccaagcaggg cgttttcgag cgttatcacc
    44221 gtacgcccgc agcactgcgc aagctgctcg agcccctggt gatgaatttt ccgctcggcg
    44281 attcaatcct tccggtgcgc aaggcgcaga gctacatccg gcaggcgcgc atcccgctgc
    44341 ccgaccgtct ggagacctac aatttcctgc atcgtgatcc gctgcaggag atcttcgagc
    44401 cggaattcct ctccgcggtc gacaccgaac gcccactgaa actcgcacgc gacgcctatt
    44461 tccgcagcgc ctcgacggaa gtcatcgagc gcatgatgca cctcgaccac aagatcaccc
    44521 tcgccgacaa cgacctgcgc aaagtcacgc ggatgtgcga ggccgccggc gtggaggtgc
    44581 gctttccatt tctcgacgac gatctcgtcg agttctcggg ccgactgacg ccgcggcaca
    44641 aggtggacaa ggggcggctg cgggtgctgt tcaaggacgc gctgcggggc tttctgccga
    44701 acgagacgat caccaagagc aagcacggct tcggcctgcc gttcggcgtc tggctgaaca
    44761 gccatggtcc gctgaagagc ctcgccgaca acgcgctcga cagcttgtcc cagcgcggaa
    44821 tcgtccgacc cgcatacctc gacgctctgc gcaaggcgca tgcatccgga catgcgagct
    44881 attacggggt gatgatctgg atcctgatcg aactcgaact ctggctgcag cacaacgcct
    44941 gaaccgaccg ctccagtccg gctccgacaa gacgctgccc cggctccgaa atgcaaggac
    45001 cgggccggcg aacacctgcc agccagcggg gcatccgcac gcgctaggcg aagaacggcc
    45061 tcacgcgagg ccacctggcg agaaggccga ccacggacag ggtgagcaga tagacgagcg
    45121 gcgtcttgag gaactcccag agcaagccgc cgccttcacg agcgccgagg acctccatca
    45181 ccgcgacatg gccggcatac acccccagcg tgtagcgtcc ccattggggc aagggcgtcc
    45241 ccaccgccca cgatggagac gacctgagca ggtttaacag cccccagacc tgaaagggcg
    45301 tggcaagcag aaaatcatgc ccctccagcg ggacaccgta ggtgtgcagc agggtccagg
    45361 cctcgacaaa agtggcgacc gtgccgccga tgaagaggaa gcaggcgacg cggcgcccga
    45421 tgcgaaccgt gtccccagcc gaccaccatc cggtggcgat gatcaacggc gcacacatga
    45481 gcccccccgg cacacccgga gaaagaggcc tcagcaacgg gtggtctgca tatgctccgg
    45541 cgccgagccc catcgcatac agcaaggcga aatagacgag gcaggggacg cgccgcttca
    45601 cgcgcatgac cgcaaacaca tgcagggtcc cgatcagcag cgcgggcagg aaccataaat
    45661 gcgcacgcgg cccggcgagc agactccagc cgtcgccgaa cagtgccgcg gcgcgccaat
    45721 aggcaaggcg gatccatccg ccttcccatg ctgcgttcca gtcgggggtc cagagcaggt
    45781 agaacgcgta ccagaagagg acaagcccgg ccagccgacc cgcaaagccg ctcacgacgc
    45841 gcaggtcgcg cccgctcttc ctgcatcgtt caccaagcag atagcccgat acgacaaaaa
    45901 agaaggggac ggcgaaccgc cccgccagat cgatcagttc ccctgcggaa acctctccaa
    45961 gtgaatacac ccgcccgatg aaggggtcac agtgaatcgc cagcacggca aagatggcga
    46021 tcgccctcgc ggcgtcgata ctgggcatcc tgccagcgac ctggggaaac actgcatgct
    46081 tgacggcatc catggaaacg gcatctcgga aaagagggag gtggagccgg aaggatcatc
    46141 ggccagggtg gattgcaagg atccgcgcct cgaccgaggc agccccgatt ctcgatccat
    46201 cgaactcatc gggacaccct gcgttcgtga ttccccgaac atcaccgcgc gaggcagcgc
    46261 tgcgggctcc cgggtcagcc gcggacaaga ccgatcgccc gtgcaaggat gcgggccaga
    46321 aggcgcaatt gcccaagttc cagcctgatc cttgcactgt tccacagata ttcgagcgcc
    46381 cgggcctgat ctccgccctt gctgctcgac caggcggcac cgaaccacaa ctcgaccagc
    46441 gcgtcacgca ccgcggcccg gccttctgct tccagcttac cctccagcaa gcgctgcttc
    46501 atcagggcgt tcgagcgcag atgctccctt tgtcgatcgg acttggacga aaacccctga
    46561 cccgatgctc gctgcatgta caggccatcg gtcacgagag cgcatctcgc ctgcagcgcg
    46621 gcacgtatcg cccagtcgca gtcccagagc aggatatctg cctggtacgg accgatccgc
    46681 tcgaaactcg aacgtctgac gaccgggcgc tgaaacgcca tcggcacagt cctcagcagt
    46741 ccccggaaca actccgccgg ctcgaacgcg aggactcccc ccctgggaga ggagaacgga
    46801 tggagggtcg acagcagcgc ccccatcgcc tgttcgtagg ccctctggcc cgcctcgctc
    46861 cgctcgccga accatgagac gcccatgaac accacatcga gctcggggtc ctccgcgagg
    46921 gccgcgactg cacgctgcag ataggacgga gcgtagagat cgtcgtcatc gaggaatgcc
    46981 agcacgtcgc cccgggcctg gaaggcgcct gcattcttcg cctcggcacc accgcggctg
    47041 cgaacgttgc ggatgagcgc gcaacggggg tcatccggta tcgacgcggg cggatccgag
    47101 gcgtcgtcga cgacgatcag atcccagttc tcccaggtct gagcgcggac acttgcgatt
    47161 gcctcggcca atagtcgagg ccgatcatga gtcgggagga cgacggtgac cgacggcgaa
    47221 cgtgtcgagg tgctcatgag cccccctcgg tgcgccggag gctcgatctc atcggtgcga
    47281 cctggagctc gcgaatcggc caccggcaac gcggggttgc gggagatggc tcttgtcggg
    47341 cggcgtcggg ccggcactcg acaatggccg ctcgagcccg gaggcgctgc ttccccgagc
    47401 ttgcacccgc ggttcggcaa tttccccgac cgcccgccga accagttcgt gcaccaccac
    47461 gatgatcacg atcaggtggt aaaacaggtc gaaatactgc aggcccagga aggagccggc
    47521 cgacatgtag ccgatcaggc tgacctggat catcgcggcg aggtcccgcg cccaggtcag
    47581 ttccttgtgc ggcttcacca tgcggatgat ctgtgtcgcc gaccgccagg tgaagatgtg
    47641 cagcagcacg aacatgacga gaccgggcca gccctgctcg ccgagcgtac cgaaccagtt
    47701 gctgtgcgaa tcggtgcctc cgaagcccgt gaccgcgcgg aaacccccgc cgagcaggct
    47761 ggagttggcg atgtagacgg cattccccca ggcgtagatg cgcccttgtg ccgagccgtc
    47821 ctcatcatag gtctggatcg tgtgcatgcg ctcgaaccac tccggcggca tcacgtagaa
    47881 cacgaccagc accaccggga tgatcccgat caggaccccg agccggttgc gcgccttgag
    47941 caggaagaac acccccatgg cgccgagccc gaccagcgca ccgcgcgaat gcgtgccgat
    48001 cacggcgatc agggtgagga cggacatggc gagcagcccc aggcgctgcc aggccacctt
    48061 gagctgcagg cggatgtacc agagcagcgg gaccgtcatc agcagggcga ggccgatcga
    48121 gttgcggtcc tcgatgaagg agccgccggg gccgcgcacg ttgtttccgc tgcccccggt
    48181 caggacgaag atgccgccct tcaccccgta gaaggccagc gagaaggcga tcacgaccac
    48241 cagccagtga acgcggtcgc ggtcgcggat cagcatcagg gtgacgtagg tcatcaacat
    48301 gatccgccac accttgtccc actgcggcca ggccaggccc ggataccacg agaaagccgt
    48361 ggtcaggaac atccatccgg tgaacaccag catcagccag gtctcgcgcg accacacgat
    48421 ctccttcttc tcgcgcgaga acaggatgcc gacgaaagtg gcgagcgcga ccatgaatgc
    48481 gaagggcatc gaggtcgaga agccccaggc catccggtgc ggattcatgt agctcagcca
    48541 cgaccacgcg aggatgccga tccacgggta tctgaggatg aagggcagcg tgccgaacac
    48601 cgcgagggtg acgatcaggt cgcgcatgac acctccgagg cgtcgacctt gctgacgacg
    48661 tagcgatact ttccggactt ctccggcgca atctcggcaa cctgctccac gtcgatccgc
    48721 accccctcgc cgagccgggc cgccagaccg tgctcgatcc gaccgacagt gtcggcgtcg
    48781 aaccccacgc ccggcaccac ctgcacccga gtgtgctcca gcgactcctg cacgatgcgg
    48841 aagcccgcca cctggggcag gtcgcgcagc acgtagatca gcgccagccc gtgcatcacg
    48901 gtgccgtcgc gggcgacgac gaagtcggtc gcgcgtccct ggatctccct cagcatcggc
    48961 aggccgcgcc cgcaggcgca ggtcgcggtg tccatcaccg ccacgtcccc ggtgcgatag
    49021 cggatgaagg gaaagtcgcc ggtggcgaga tgggtgacga cgatctcgcc cgcctgcccg
    49081 ggcggcagca ccctgccctc gccatcgacg atctcgacaa tgaggtcctc ggcagtgagg
    49141 tgcatgccgc cttgcgggca ctggtgggcg atgaagccgg cgtcgcggga gccgtagccg
    49201 ttggcgaccg ggcagccgaa gacacgctcg atggcctcgc gctggtggtc gtagaggcgc
    49261 tcggaggtgc agaacgccac ctcgatgccg aggtcgtcca tgcgaatccc gcgcctttcg
    49321 gcgtgcaggc cgatatggga cagcgccgaa gggtagccga agagcatctt cgggcggcgc
    49381 gcgcggatgc gggcgacgaa gccgtccagc ttcgtggcgg acatctcgaa ggcgggcagc
    49441 agctcggtac ggaacagccg atcccgcagt gcgcgcaccc ggtcctgcgc gccgagctcg
    49501 atcggcgagc cccacacgac aatctcggga tcgccgatgt cgacgcccca ccagcgcgtc
    49561 gcgcgccact tggcggcgac gtcgtggctc acacgccggt cgccgatgaa gaagatgagc
    49621 ggctcgccgc tcgagccgcc ggtgttgaag cgagccagtc cgaccgcgtc gtcggccttc
    49681 aaggcttcgg tattggcgcg gatctccgcc ttgcccagga agggcaggcg ctgcagctcg
    49741 gccaggctgg cgatcgccgc ggggtcgaag ccgatgcgcg cgaagccttc gcggtaatag
    49801 ggcacgtggt cgcgcgcatg caccagcagc gcacgcaggc gctcgagctg cagggcctcg
    49861 atgcgctcgc gcggccacca ctggctatcc tccaggccgc gccgcagggc gacgctgtcg
    49921 tgttgcttga ggcgctcgtg gagcgggaaa agcaggcccg agaccaggcg ggtgtagagg
    49981 ccggacattt cgttttcctc cgcctcaagg gttcgcggtg cgatccagca tggcctgcag
    50041 gggcccgccc atctcgcgtg cgaagtcttc gagcgcggcg atcgtgccgg cgtgtccgcc
    50101 ctgaccctgc ggcgcatgca gcatgaccac cgccgaatcg tccccttcgc cgcgcaggcg
    50161 gctcagcgcc agatagacct tggcgagatg atcgctggtg gtccaccggc cgccgatcca
    50221 gtacgcgtac cagacgacca tgcgtgcatg ctcgctcgcc atctccagcg tgcgccacgc
    50281 ctgcgcctgg tcgccgagtt gcacccggac cggacccgcg gcggtcatgc gccacaccgg
    50341 gtccttgctg atcagcacgc ggttctcgga attgatcaac tccctgcccg gtccctggtc
    50401 gcggtaatag ccgatgtaca cccccaccgg aacgctcccc gcgcgccagg tcgaacggaa
    50461 ggtgtcgcgc atgccggaat agctcggcga ccacggcggc aattcgcccg cttccgtccg
    50521 ctgccacgcg ccctgcccct ccagcgcggc gaagcgcacc ggcccctgct cgccctggcg
    50581 ctcgaggtgg ccgaggaccg gcacccacag ccccaccgcg accaccgcca cggccagcca
    50641 gcccaggcca ccggtgcgta cgcccgacga tcgcgcggag ccgacgggca tggccggggc
    50701 ctcttcctcg tcctcctgcc agcgcgaacc gatccagaac agcgcgagga tga
//
LOCUS       DAOQBS010000009        45039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_403_length_45039_cov_17.651165, whole genome shotgun sequence.
ACCESSION   DAOQBS010000009 DAOQBS010000000
VERSION     DAOQBS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 45039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45039
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_403_length_45039_cov_17.651165"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PKN25_02140"
     CDS             complement(<1..839)
                     /locus_tag="PKN25_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HNV89726.1"
                     /translation="MIDHRLLAFEAVLQEGGFERAARRLALTQSAVSQRVKLLEAELG
                     QVLLVRSKPVRPTPAGRRLLPYLAQLRLMEAEARRALSPREVDGPLRLAVGVNADSLA
                     TWFIGAVAEVVRDEGIVLDCVVDDQDHTHALLADGEVLGCVSTRADPMRGCAAVRLGA
                     MPYLCAGSPDFRARWFPQGLTPAALARAPAIVFGHHDDMHEAFLLRHFGLDSRRYPHH
                     VVPSSEGFMAFALAGLGYGFVPEIQARAHLARGELVDLAPEREEVVLYWHHWQVQSPV
                     MARL"
     gene            1018..1623
                     /locus_tag="PKN25_02145"
     CDS             1018..1623
                     /locus_tag="PKN25_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HNV89727.1"
                     /translation="MSNVYLNGFLLTLGLIMAIGAQNAHVLRQGLRGEHVLLTIGVSA
                     ACDILLIGLGIAGVGSLFVANPDLMTVARYGGAAFLAWYGARALRSALRGGRALDLAE
                     HKRMSRGQALLAAAGFSLLNPHAYLDTVVLLGSIGSQQAEDLRPLFAAGAISASIVWF
                     LLLGYGARLLAPLFAKPVAWRVLDALVALMLWAIAGSLLLG"
     gene            1857..2747
                     /gene="asd"
                     /locus_tag="PKN25_02150"
     CDS             1857..2747
                     /gene="asd"
                     /locus_tag="PKN25_02150"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586105.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNV89728.1"
                     /translation="MSERLAVALQYALPKRLLTQLAGKLAGLEGGKATTAVIRWFAKR
                     YGVNMAEAANADPASYASFNQFFTRPLREGVRPIADADFVCPVDGAISQFGPIEFDQV
                     FQAKGHFYSTTALVGGDRALAARFENGDFATIYLSPRDYHRIHMPCAGRLTQMIHVPG
                     ELFSVNPATARGVPGLFARNERVVCVFEGEFGPFVMVLVGATIVGSMATVWHGVVNPP
                     RRPDIVKRGYAEGEVVLEKGAEMGRFLLGSTVVMLFPQDVLRFGSDWAPGGPVRMGEP
                     MARAKSGAPSDGAPALADAP"
     gene            2934..3368
                     /locus_tag="PKN25_02155"
     CDS             2934..3368
                     /locus_tag="PKN25_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV89729.1"
                     /translation="MPSRPIHQILSDRPFVGVSADTPVREVVRQMNALQRSAALVTEH
                     GVLTGIFTERDAAFGVLAAGLDADTTPVGAVMTHNPVTLTEDRPFGHALHLMYENGVR
                     HVPIVDANRRPLGVVTARDALQLDALTFGAELVRREEITVIL"
     gene            complement(3521..4819)
                     /locus_tag="PKN25_02160"
     CDS             complement(3521..4819)
                     /locus_tag="PKN25_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNV89730.1"
                     /translation="MSKLQALYDSKRMTASDAAGQVRDGDTVVVPTGVGEPPALLHAL
                     SARRHALRDVAVSQILPLRKFAYLDPETRANVRHDAYFFGGANRAGGQAGWVDYVPAY
                     FSELPMLIERGLSPADVVVSLASPMDEHGYFSLSLAPDYTMAAIRRARVVLLEVNPNV
                     PFANGDCLVHISRVAGLVESDDELFEVGLPEIGPVQQAIGKYVAELIDDGSTLQIGYG
                     GIPDAVVVQLQHKRELGIHTEMLGDGILSLIESGAVTNRKKTFMPGRTIATFALGSRR
                     LYRTMHRNPGIEMHPVEFTNDPYLAARNDKLCAINATMQIDLIGQCGSESLGHLPYSG
                     TGGQADFVRAANRSKDGKAFIVLPSTAKKGTVSRIAPVLSPGTHVTTSKNDINYVVTE
                     YGVAQLRGKTAKQRAEALIGIAHPDFRGELRDAARKMNLL"
     gene            complement(4908..5894)
                     /gene="cobJ"
                     /locus_tag="PKN25_02165"
     CDS             complement(4908..5894)
                     /gene="cobJ"
                     /locus_tag="PKN25_02165"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HNV89731.1"
                     /translation="MNAPETIVPPGTSAPAGKIMLVGLGPGAHAHLTERARAAIAEAD
                     TIIGYVTYIRLVADLLEGKEVIRKSMTEELDRAIEALERARQGRKVALVSSGDAGVYG
                     MAGPTFEVLFQAGWTPPSGKPGDIEVEIVPGASALNTCAALVGAPLTHDFCAISLSDL
                     LTPWPAIARRLDAAAYADFVVALYNPKSGRRTGQIVEAQRIFLRHRDPATPVAIVKSA
                     YRPRQRIEFSTLERMAEADIGMLTTVLIGNSNTFVRDGLMVTPRGYANKYAVDGSERA
                     TRAGEQAGRSLSSGLDGWLATIRASGRNARELAAEYRLPEDYIRAALAHPPA"
     gene            complement(5978..6415)
                     /locus_tag="PKN25_02170"
     CDS             complement(5978..6415)
                     /locus_tag="PKN25_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HNV89732.1"
                     /translation="MAAAKKLALGLGCDRGTPAATITRAIDAALARIGASRIDVAAVA
                     SITLKADEAGLLEAATAAGWTLRFYPPAVLAAVDVPNPSETVRRYTGTPSVSEAAALL
                     AAGTALATDPGALRVEKHKLRGPDGRNATVSIARLPASSPAPN"
     gene            complement(6428..7183)
                     /locus_tag="PKN25_02175"
                     /pseudo
     CDS             complement(6428..7183)
                     /locus_tag="PKN25_02175"
                     /inference="COORDINATES: protein motif:HMM:NF023189.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis central domain-containing
                     protein"
     gene            complement(7195..8073)
                     /gene="cobI"
                     /locus_tag="PKN25_02180"
     CDS             complement(7195..8073)
                     /gene="cobI"
                     /locus_tag="PKN25_02180"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HNV89733.1"
                     /translation="MNPHPANTARFGRLIGISLGPGDPELVTRRGWAALQSDARWAYP
                     VKKAEESSYALDIVRRGGLSIPADAVELVFPMTRDAVALAKAWARAATQVVALLAEGR
                     DLAFLVEGDASTYSTFRHLARTVRELAPEVEVDTIPGVSSFAAAAAVADLALAEEDET
                     MAVIPAAYGTTVIDHLLDEFDTLVLMKVKPLLDEVLDLLERRGLLATSCFVEKVGAPA
                     QRVVHDLASLRGAQVNYLSLLLVQNPKRARGELRRGCRKRAAQVEPQATAQAPSPDTG
                     GAAAHPGESPAATALS"
     gene            complement(8070..9386)
                     /gene="cbiE"
                     /locus_tag="PKN25_02185"
     CDS             complement(8070..9386)
                     /gene="cbiE"
                     /locus_tag="PKN25_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HNV89734.1"
                     /translation="MPESTAPVCSIVGLLDDGWDGLGAAARRRIEAAASLIGAGRTLE
                     RIAPHAPSARLLNMDGALTRVPGWIEEAVLEGDAAVVLATGDPLCHGIAGFLIDKLGA
                     ARLEVLPATSTLQLAFARLKRSWQEARFVSCHGADADEWQADPRQPGPTPEHGLYRVV
                     RAVAEHPLVACFTSPANSPDRIARALLAAGHGAPGCDEDLRLSVVARLCLDDEAVFAG
                     LSLQEAASRRFPEPNVVVIERTCIGTPLFGVDDLDYVQRTPEKGLITKLEARAVALAK
                     LALRADSRVWDIGAGSGSVGLEASRIARHGHVWAIEKNPADAANARTNARRLRASNYS
                     LFEGKAPAGLDAWPDPDAVFIGGSGGELGELITLVLRRLVPGGRLVMNFVTLENLATA
                     TSVLAAADTAWEVTMLSAARSQPILDMHRLAAQNPVWIVTTRKEAP"
     gene            complement(9379..10545)
                     /locus_tag="PKN25_02190"
     CDS             complement(9379..10545)
                     /locus_tag="PKN25_02190"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HNV89735.1"
                     /translation="MAAGHSLPDKVRKGDARRARGNRTGLTTGANSAAAAAAATLGLV
                     RGAVPDAVECVLPNTTRVRFTIRDGQVDGDHAHAVSIKDAGDDPDATHGARLTADVRR
                     IRGGGGVVILAGGPGVGVVTKPGLGLAVGGPAINPVPRRNIIDNVRAAGTPILEAGDG
                     LEVTISVPGGEEIARKTLNARLGILGGISILGTTGIVRPYSTAAFRASVIQAIDVAAN
                     QGQTCVVFTTGGRTEKCAMRAFPDLDEACFVQMGDFVKAAFTTAVRQGMRHIVVGAMV
                     GKLTKIAQGLSVTHAWREEVDRELIAAAAAEVGAPPALVAEIRAAETARFAAERLSAL
                     GLAVAFHRALAGRAIRSLRQRYPGPHRLTVLACNFEGVPIVSVDEADLKETTHA"
     gene            complement(10555..11235)
                     /locus_tag="PKN25_02195"
     CDS             complement(10555..11235)
                     /locus_tag="PKN25_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HNV89736.1"
                     /translation="MRTNTVTEQLTAAGRAIEHDSFAIIDAEVGRHAYTAGQWPVVRR
                     MIHANADFEFNGLTEFHPDAVDAGLAAILGGGARIVADVEMICVGLSASRLAHFGMRT
                     HQFISDPDVVEHARAEDSTRAVQAMRKAHRLGLLDGAIVGIGNAPTALIELVRLIRDE
                     GVRPALVVGMPVGFVSAAESKDRMAAVDAVPWIVIRGRKGGSTLVVAAIHALLGLAET
                     RQRQAVRG"
     gene            complement(11249..12163)
                     /locus_tag="PKN25_02200"
     CDS             complement(11249..12163)
                     /locus_tag="PKN25_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HNV89737.1"
                     /translation="MNKTAILLVGHGSRNREGNKEILHFAAQWRERHPGWRIETCFIE
                     HADVLLDEGLDRAAHAAHRVVTIPFILNAAGHVKMELPAAIERARARHPGVAFACARH
                     LGMGRELFAVLRGQLDRLLRQLDMPDPHTTGVVLLGRGSSDAGANGEFAKMARWLFED
                     GDHELVDLAFTGVTWPRLETVVQRQARLGMTQICIVPVYLFTGVLMERIRAQVERLRR
                     QYPQLAFALGTHFGFDEGIFALLDRRVDDHEAPEGGLLECDGCRYRLAAEAVHLHEHS
                     PTHARHAPAHHEHDHGHAYARAHGHAHA"
     gene            complement(12160..12987)
                     /gene="cobM"
                     /locus_tag="PKN25_02205"
     CDS             complement(12160..12987)
                     /gene="cobM"
                     /locus_tag="PKN25_02205"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HNV89738.1"
                     /translation="MTALPGTVWFVGAGPGDPDLITVKGRRLLERAGAILFAGSLVDR
                     SATQFAPAHCEIRDSRDMTLEGMSAWLLDACARHPVVVRLQTGDPGLYGALVEMTRPL
                     DAAGIPWRVVPGVSSAMAAAAAAGETLTLPEVTQTVILTRVAGRTPMPPGEALEALAA
                     HRTTLCIFLSITLLHEVQRALRAAGWPEDAPIVVVHKASWAGAEKIVRGTLADIKRRC
                     QDEKIASQAMILASPALGARLWPDLARSKLYDPGFGHRFRKASMPAAAAGIATGETA"
     gene            complement(12987..13625)
                     /locus_tag="PKN25_02210"
     CDS             complement(12987..13625)
                     /locus_tag="PKN25_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNV89739.1"
                     /translation="MHIEPGILSGAKIAAANLAATALVAAQAPQLLRKPQRLLRTLLA
                     ALFFSVFMQSFHTPAGASELHFIGAMPIYLTLGFVPTLLGFGLGLLLQGGVFEPADLL
                     HLGVNFLSLAVPLVALHATLGRRLASGAATRVGSVLKLDAAYYAGVAVMVGFWLAMGE
                     VATPFAAWAAFASAYLPVALIEPVLTVALVRLLQSQAHRPLVRLCFAVPARG"
     regulatory      complement(13894..14118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(14294..15874)
                     /locus_tag="PKN25_02215"
     CDS             complement(14294..15874)
                     /locus_tag="PKN25_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV89740.1"
                     /translation="MNPQVTFASLGLAEPILRAVSEAGYSTPTPIQAKAIPQVLAGGD
                     LLAAAQTGTGKTAGFTLPLLHILSQTHTHPHPPGRPRCLILTPTRELAAQVQESVDTY
                     GKHLPLSSLVMFGGVNINPQISALKKRVDILVATPGRLLDHVGQKTLDLSGVEILVLD
                     EADRMLDMGFIRDIRKVLALLPKKRQNLLFSATFSDEIRELAHGLLHNPAEVEVAPRN
                     TTAERVDQAVYLIGQKQKRELLAHLIKEKQWFQALVFTRTKHGANKLAEYLTKHDIGA
                     AAIHGNKSQAARTRALAQFKDGSLPVLVATDIAARGLDIDQLPQVVNFELPNVAEDYV
                     HRIGRTGRAGAAGNAISLVDNEEIKLLTAIERVIKRRIERAVAEDFVPSAGGEAAHDA
                     DHQREPLQRRGGRNGQGRSPAAAPARSERAGGGRAPAQPGGRAPARTRGEADGNRAAR
                     TGEAREPNGNRGSAPRPDVDGNRAVPARRVEVDGNRAPSANRRPEGNAPRPAGANPQR
                     RPQPRAALFAAKPGGDKR"
     gene            16179..17333
                     /locus_tag="PKN25_02220"
     CDS             16179..17333
                     /locus_tag="PKN25_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV89741.1"
                     /translation="MREIQLSEDLDGRYDRLSRDLGARALAGLLAVSGSILVLLGGAV
                     VGAVATWEAIGRGTPVETLFWVLFMLGSIAAIWATLASLASLAPRPCGLAVARERVGE
                     LYALVDALAHVAGVSPIRRIYVTGEINASIVQRPRHLGVGEMATDLLIGLPLAHALGP
                     RQLAAVIAHEIGHMVARQRGMHGRSGWLQAWWMRTLDELAAALPSGFPWFDPQGDALC
                     MRMLLLAQMEEYAADRTAVRLVGAELLAGTLVELCCKADFLANDYLPRVHALAECDEA
                     SCVRPYREMGHGFAAGFAQSSAAIDPQRVLRADAGDPFHPALQDRLVAIGVAMPERLQ
                     VSGPSAAQHFFGDSLPFLAWHFDRIWLESLRDACPPALSPRAPERVLQGA"
     gene            complement(17293..18258)
                     /locus_tag="PKN25_02225"
     CDS             complement(17293..18258)
                     /locus_tag="PKN25_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNV89742.1"
                     /translation="MSSLPCRLSDAAASAAAATLLALGGPASAAGVELVDDTGRKLAL
                     AAPAQRIVSLAPHVTEMLFAAGAGERVVGAVDYSDYPEAAQRIARVGGYTRIDLEAVA
                     ALRPDLVIGWQSGNREGDLARLQALGIPVYLSEPRNLEDVARNLERLGQLAGSEPAAQ
                     AAASAFRARREHLAATYSARDKVRVFYQIWDRPLMTVNDHHLIADVIRLCGGANVFGE
                     VAHLTPTIGVEAVLAANPEVIVASGMGEARPEWLDQWSRWPQLEAARRDNLFFIPPEL
                     IQRHTPRILDGAARLCGQVETARKRRGGASAALTPPAAPAPARAD"
     gene            complement(18258..19247)
                     /gene="cobD"
                     /locus_tag="PKN25_02230"
     CDS             complement(18258..19247)
                     /gene="cobD"
                     /locus_tag="PKN25_02230"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HNV89743.1"
                     /translation="MLEHGGRLRRAAEHYGIALADWMDLSTGISPWAYPVPPVPETAW
                     QRLPEDDDGLDEAAARYYGSAELLAVAGSQPAIQALPAVLPGARVRLLAPGYAEHAHA
                     WRERRPRSIDAEEVEGAIDDSDVVVLVQPNNPTGVHFDRDRLLDWHARLARRGGWLVV
                     DEAFADTTPAHSLVPLAGRPGLVVLRSLGKFFGLAGARVGFVFAPTEVRTALAERLGP
                     WTLAGPARWAAHHALADTAWQAAQRARLDAAGARLHRLLQAHGLESCGPALFQYVRLP
                     HAAQVHAAFARRGILLRRFDAPPALRFGLPADEPRWARLEAALADCPNLLKEP"
     gene            complement(19240..20205)
                     /gene="cbiB"
                     /locus_tag="PKN25_02235"
     CDS             complement(19240..20205)
                     /gene="cbiB"
                     /locus_tag="PKN25_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HNV89744.1"
                     /translation="MPSSLLLVLTLKMAAGLLADRVFGEARRFHPLVGFGRWAGWVER
                     ACRRLFFGTNETGMRLAGLLAWALAVLPWVALALWLRTLHPHAHWGVDSALLYFALGG
                     RSLAEHALAVATPLAAGDLDAARARVGWIVSRDTRALDAEGVAKAATESVLENGNDAV
                     FGALLWFVLGGGAGVVLFRLANTLDAMWGYRTPRHLHFGWAAARIDDLLGWLPARLTA
                     LTYAWLGDTRRALACWRAQAPAWDSPNAGPVMAAGAGALGVRLGGAATYHGRAEHRPA
                     LGEGRGADVDAIRAAVALVRRGALLWLGLTAVLGVATTLAGGGGA"
     gene            20382..21917
                     /locus_tag="PKN25_02240"
     CDS             20382..21917
                     /locus_tag="PKN25_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNV89745.1"
                     /translation="MSPPITLMVQGTTSDAGKTTLVAGLARVLRRRGIKVAPFKPQNM
                     ALNSAVTIDGGEIGRAQALQALAAGVAPHTDFNPVLLKPSTDIGAQVVIHGRAVASLS
                     ARDYHAYKPTAMAAVMQSWDRLCSAYAAVLVEGAGSPAEINLRDRDIANMGFAEAADV
                     PVVLVADIDRGGVFAHLVGTLELLSPSEQARVKGFVINRFRGDLGLLQSGLDWLEART
                     GRPVFGVLPYLHGLFLDAEDALADSAADVRAGEAALRVIAPVYSRISNHTDLDPLRLH
                     PQVDFRWIGPGQAIPPADLIVLPGSKSVHADLAWLRAQGWETAIRRHLRYGGKLIGIC
                     GGFQMLGRELSDPLGLEGAPGTMAGLGLLEVDTVLEQHKQLRNLRGRLANLSREDGSE
                     CAGEPGAAVTGYEIHMGVSSGPALARPALWLAHGDEAMPTRPDGARSADGQILGTYVH
                     GLFDAPAALAALLAWAGLDAEAMEGSGPVDLAARREADLDRLADAIEAHVDLASLFAP
                     NRL"
     gene            21939..22784
                     /gene="queF"
                     /locus_tag="PKN25_02245"
     CDS             21939..22784
                     /gene="queF"
                     /locus_tag="PKN25_02245"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321992.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HNV89746.1"
                     /translation="MNTDTPRRHGAESSPLGQAVAYRDTYAPELLFPISRQLKRDELG
                     LRADMLPFCGEDLWNAYELSWLDARGKPVVALGEFRVPAASPRLIESKSLKLYLNSFN
                     QQRCASVEEVRATIARDLSAAAGAAVAVVLRPLQERPGRSFGYPEGVCIDALEIDIGR
                     YQPCAETLRAAGPEVEETLYSHLLKSNCLVTGQPDWGTLVVRYRGAAIDREGLLRYVV
                     SFRGHNEFHEQCVERVFCDLMARCKPQELAVWARYTRRGGLDINPFRASRAGLRADEA
                     MEVRQ"
     gene            22891..23199
                     /locus_tag="PKN25_02250"
     CDS             22891..23199
                     /locus_tag="PKN25_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV89747.1"
                     /translation="MKALVAAAVAAGLLSASPAFASEALAKSKNCLACHAVDKKLVGP
                     SYKEVAAKYAGQADAAAMLADKVQKGGVGAWGKVPMPPNPQVNAADAKALVDWILTLK
                     "
     gene            complement(23263..25467)
                     /locus_tag="PKN25_02255"
     CDS             complement(23263..25467)
                     /locus_tag="PKN25_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV89748.1"
                     /translation="MVTPADNGLRPRIDATAGDDADSVRILIVDDDPALRRTFPHALA
                     RPGRLFDECGSIGEAIGRLEKQRYALILLDYRLPDANGLALLDWLSDHQRDEAVIIIS
                     GEDAIDAAIGALRGGADDYVRKPYHVAQLQRAVDGALHKGTLERANKLMSQRLKDSER
                     LHRYLVESSPDMIFTLDGSGRFTYINPRIKALLGYDRSALVKRPFTTLVMPEDLDRID
                     TLLSQPSSLPGESFHVELRLRSRRDGGGEGGSVTVSLTGIPMVTQENDRRVIGLYGVA
                     RDISERKRAEEIISFQAYHDQLTHLPNRVLFKDRLELAIAQAQRRTGALAVMFIDIDR
                     FKLVNDTFGHAEGDLLLRAIASRLSATLRRGDTLARLGGDEFTVLLPDINQPEDAEVI
                     ARKVLDALATPVRLSQGEFRATVSVGIALFPRDGTTAEDLTRHADAAMYQVKRSGKNA
                     FRFFDPELNTRHRDRIALENDLRGAVARGELDLHYQPQVSLATRGIVGLEALLRWNHP
                     GLGAIPPATFIQVAEEVGLISEISNWVLETACAQLARWQAEGVGAPRVSINLSACDFQ
                     RGDVVGRIRACLDRHGVAPSCLEVEITEGLMMDDTADIAAKVRALREAGISVAIDDFG
                     TGYSALAYLQRFPVSTLKIDRSFVRDLEGPMTNPIISAITGIARGFDLDLVAEGVENL
                     EQADALRALGCEVMQGYLFARPAPAGEVPGWLRQPERLFLGAGSADLQAAPA"
     gene            complement(25520..27484)
                     /locus_tag="PKN25_02260"
     CDS             complement(25520..27484)
                     /locus_tag="PKN25_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNV89749.1"
                     /translation="MFAANFSAFELQPLPDGASTILRMLAAQTPNWAEIALVAARDPA
                     LSLALLLADPLRAGELQDGLNAALRRRLERIGTDLLRAWLLGLGHVGNQPDETADAAL
                     LRAECALHLAIETEYPRPDEAYLAGLWFGLPRSAGWVSGRRPTLPALVADCGLPASLA
                     DALEFDGMDPACAGAMHPLLGLLAAARRLAGGDWQERITEVAELTGLESASVVALRTD
                     VGYILSGHASYPALPGLPSAGTGAACPTLRLTDDPYRYAGMLGLLTAAFVDLDATTIR
                     DRLAIACPLFGLHTPPVLLGSGEDGRLRPLLGAAAGSPDLLIGELRLRLDDETSCIAL
                     GARSEQPCSHFGDGGPPGRSVADWQVARWLGQPGFHVIPLGGAGDTSVALVATASAQA
                     LDSELRWRYAALLGAAARALRSHARQRDAIAEREAALHQRFRDHVRRIAHEASNPLTV
                     LKTRIDMLAQERSGDSTLQDEMSLLNAELDRIDKLLRSAAELPAETAEMRTCRVPELL
                     LDMRTLYGEPLFGSRQIQLELRAARDVPPAAIPASALKQVLLNLLRNASEALQPGQRL
                     VVSVLPLVNVDGRNCLEIRFVDNGPGLPVERAQDPLSPRPSAKGAEHQGLGLSVVREI
                     LAQWGGTLLCRTQAGAGTSFQIFVPLEQSA"
     gene            complement(27601..28737)
                     /locus_tag="PKN25_02265"
     CDS             complement(27601..28737)
                     /locus_tag="PKN25_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV89750.1"
                     /translation="MSQTTLLTREPVVNKNRAITANRLIAHGPNIASVVEALSAQAAV
                     WPTHHPVFVSLGRLVPTPEIMEWAIPGNVAIEIPVQTLAHPHTQALMARLRDAGIGMC
                     LTWFQPEVALPAGHDWRFVLIDARRHPAPTGSPGLSLAWGLADIGAFRQAVAAGYDGA
                     SGWFFLHGNPPAKSLTPGHAQIVRLLNLVRNNGDIREIEGVLKQDVALSFKLLRYINS
                     AGFGLMVEIQSFRHAVSILGYDALNKWLSLLLVTASRDPSAPALMQTAIARGRFMEEI
                     GGGFFDAVERDNLFITGAFSLLHTLLGTSIQTLLEEMNLPPRVSEALLYGQGDFAPFL
                     HLAQCCESFDPKALQAAVDQLHLSCEQVNRAQLVGLGFADSLHG"
     gene            complement(28751..29764)
                     /locus_tag="PKN25_02270"
     CDS             complement(28751..29764)
                     /locus_tag="PKN25_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HNV89751.1"
                     /translation="MSHRISLQPGNHSFEADDDQTVLEAALAAGLLLPHGCRDGACGA
                     CKGRVLEGEVEHGEHSPGALSEAERAEGLALFCCAKPLGDVMVQARSVTRAGDIQVKK
                     LPCRVQKLERLAEDVMRIELKLPASENFAFRAGQYIDILLADGQRRSFSIANAPHDAR
                     HLELHVRRIDGGRFTGHVFETMMEKEILRFEGPLGSFFLREDSARPIVLVAGGTGFAP
                     IKGIVEHAIRLGLQRPITLYWGARRRDGLYLDALARAWEEVLPGLRYVPVLSDEAWAG
                     RGGLVHQAVLEDFADLSAHEVYVCGAPAMVDAARASFHSERGLPEDAFFADAFTFAQP
                     SAR"
     gene            29786..30661
                     /locus_tag="PKN25_02275"
     CDS             29786..30661
                     /locus_tag="PKN25_02275"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV89752.1"
                     /translation="MKRILIVGSGDVARRALPWLVRRFRVFALVRQPEAAAELRALGA
                     VPVAGDLDDRRSLRRLAGIADAVLHFAPPPQTGEGDPRMARLLAALAGRSSLPQGVVY
                     ISTTGVYGDCAGVRVDETRPCRAQTARGRRRVDAERRLRAFGRRNRVRVALLRAPGIY
                     AADRLPLERLRRGDPVLAADEDVHTNHIHAEDLARIACLALFRAGPGRAYNASDDSGL
                     RMGEYFDAVAERFGLARPPRMARAEIVRHLSPLALSFMSESRRLDNRRLKRELRLHLR
                     YPTVADGLRAATVPV"
     gene            30723..31133
                     /locus_tag="PKN25_02280"
     CDS             30723..31133
                     /locus_tag="PKN25_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNV89753.1"
                     /translation="MLTLKAFHIVFVVSWFAGLFYLPRLFVNHAMVEDAATRERLAMM
                     EMKLYRFMTPLGILAVGLGLWLWFGYGFAGGWLHVKTLLVVFLIAYHLYCGRLMRAFA
                     EGRNTRSHVWYRYFNEIPVLVLFVVVFLVVLKPF"
     gene            31136..32641
                     /locus_tag="PKN25_02285"
     CDS             31136..32641
                     /locus_tag="PKN25_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV89754.1"
                     /translation="MVAKRIGGGGDDVQMAHGVRGDEAKALAQGGVGRAEPARPVELA
                     DYQWPAHSVLVVDDEEGMRNFLERTLARRCGMVQSAADAEHAAVLMARLHFDLLVLDI
                     ALPGKSGIEWLHELRENGYAGDVILITAFADMETAIDALRGGASDFILKPFRVDQILN
                     SIKRCFERAGLARENFVLRRELAGLGAEPTGLIGHSPAMEQLRALVRRVGQMPSTVLL
                     LGESGTGKEVVARALHQTSPRAQRPFVPLNCAAIASELIESELFGHVKGAFTGATENR
                     NGLFYYAHGGTLFLDEISELPLAMQTRLLRVLEERKLRPVGSERELPVDVRIIAASNR
                     DLAAEVRAGRFREDLYYRLAVVDIGLPPLRDRTEDIPELMRHFMQQFSVQLGVPPLPL
                     SHEVVSRLAGYTWPGNVRELRNYIERSLILGHFPAQPSAAPTAAPTPAGELEASLAEV
                     ERRHIERVTAACEGNKTEAARRLGVSRKTLERKFAEWALEDAAAARAERRA"
     gene            32706..34787
                     /locus_tag="PKN25_02290"
     CDS             32706..34787
                     /locus_tag="PKN25_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNV89755.1"
                     /translation="MRLAERLRGSVRAKLLALVLAPLMLGFPLIMGVLWYWGEVYYHR
                     LMVSRVASDLATAHGYFERVIDGVGNGVQGLAGSHALAQTLAHESPEAVAALLGARKG
                     GLALDFLNLLDVNGRVLHASTALAAGSARGGWPVVQQAIRARGGSVVERFAPAELAAI
                     DAGMRERARLALVATARARPDSRGEEERGLVVHAAAPVLDAAGNLVAVLEGGVLLNGN
                     LDFVDTLNAIVYREGSLPEGSEGTATLFVDDVRIATNVRLFEGARALGTRASDEVRTH
                     VLDHGRTWLETAFVVNDWYVSAYEPVVDSRGERVGMLYVGFLEAPFRAAKRMALLVVG
                     ALALLVSAAGALLTLYWARGIFRPIERMHATIGRIDAGDEQARVGDVASRDELGRLAT
                     AFDHLLDDQAARRAELQALNASLDRKVAERTADLADANAELRAAQHRLVMSEKLAAIG
                     ELTAGVAHEISNPTAVIQGNLDLLREELGPAAQPVANEIRLIHEQVGRIRLIVTKLLQ
                     FARPGEFAGYVEDVDVNAALADCLVLTRQHLARAEVKVVQRLAATARVQINLQELQQV
                     LINLIVNAVQAMPAGGTLTLETVDRDPRQDARGVRITVRDTGGGIRAEDLARIFDPFF
                     TTKKRQGTGLGLSISHTLVERYGGRIEVDSAPGRGAAFTVSLLAEPVYRKDPAPPPGG
                     RENGTGQTEAT"
     gene            34784..35275
                     /gene="mobB"
                     /locus_tag="PKN25_02295"
     CDS             34784..35275
                     /gene="mobB"
                     /locus_tag="PKN25_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNV89756.1"
                     /translation="MKVMGFAGWSGSGKTTLVEQVIAALSARGLVVSLVKHAHHSFDI
                     DHAGKDSWRHRQAGCREVMVSSDQRWSLTRELRGGPEATLDELLAHLGPCDLVLVEGF
                     KRAAIPKIEVHRRGLAEPLLYPHDPHIVAIATDAELPAPLPRLDLNDPVQVADFIADH
                     LHL"
     gene            35383..36717
                     /locus_tag="PKN25_02300"
     CDS             35383..36717
                     /locus_tag="PKN25_02300"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV89757.1"
                     /translation="MRIEKTTAGTPHRIVIVGGGAGGLELATRLGRKFGRGGRVEVTL
                     VDSKRTHVWKPKLHEIAAGSMDIGDHEVPYLAQAHWHGFRFRIGQMIGLDRSRREIHV
                     APFVDEDGKEVTPARSFPYDTLVIAVGSLSNDFGTPGVREHALKLETVADARRFHVRM
                     VNACVRAHAQRSPLRPGQLHVAIIGAGATGVELAAELHHTTREVVAYGLDRVDAEKDV
                     RLTLIEAAPRVLPALPERLSRSTERLLRKLGVEVNTDARVAEVLPDGVRLADGRVLPA
                     ELVVWAAGVKAPDFLTRLDGLETNRINQLVVRQTLQSSVDDDIFAMGDCAACPWPEKD
                     GFVPPRAQAAHQQASHLYTQLQRRLAGKPVQDYRYRDFGSLVSLGEFSTVGNMMGGLA
                     RGDLFIEGWFARLMYASLYKMHELALHGWVKVTLDTLARMITRRTEPHVKLH"
     gene            complement(36737..37660)
                     /locus_tag="PKN25_02305"
     CDS             complement(36737..37660)
                     /locus_tag="PKN25_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNV89758.1"
                     /translation="MSARPAQPAGTDAASATCLNAREAAAFLQLNEKKLYELANSREI
                     PAARVGGKWLFPRALLEEWLLEQAHGGALSDRLLITGSDDPLLAATVGALAPVLGGDA
                     FVAYSPTGTLPGLELLARRRASVCALHWGGVEHSLQQHAMLLRRFAPHRHWALVRLAL
                     REQGVILRRGLAVDGIETLAAFDYRWAMRQAGAGSAHFLESVLASRGFSAADCSVVAT
                     ARSEREAAALVAREDADCAPGTRAAATEFGLGFLPLGWEAFDLALPRDVMFRRLFQDL
                     LAAHGDARSQALAHRLGGYDLGPLGRVLGLD"
     gene            complement(37657..38298)
                     /gene="mobA"
                     /locus_tag="PKN25_02310"
     CDS             complement(37657..38298)
                     /gene="mobA"
                     /locus_tag="PKN25_02310"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HNV89759.1"
                     /translation="MPPSPAPRPADRRITGILLAGGQGSRMGGVDKGLVELAGRPMAA
                     HALERLAPQVDALLINANQNLPAWQAFGHPVFGDDIGGFAGPLAGLHAGLLRAQHPFV
                     VTAPCDSPFLPADLVERLAAALHAADAQLAVAKTFGQPHPVFCLCRRNLADHLGAFLA
                     AGGRKIDRWYGSLKVVEVAFDDEEAAFRNVNTRDELAEAARDLPAAAAGAPRG"
     gene            complement(38335..39162)
                     /locus_tag="PKN25_02315"
     CDS             complement(38335..39162)
                     /locus_tag="PKN25_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNV89760.1"
                     /translation="MTPPHRPLLSQARRPLTIEIPAVDERGDTVPTSIAGEHPLTLYV
                     DKREIVTLMTLGAAPEALAIGWLRNQRLVRSLDEIAAVQVDWDTDAVAITTRAGMQDA
                     ERKLGSRTVTTGCGQGTVFGGLMDEIDQIRLPHDRQLRQSTLYALMDAVRHHESIYKQ
                     AGAVHGCALARATPGGCEILMFFEDVGRHNAVDAIAGQMWLDGLDGSDKIFYTTGRLT
                     SEMVIKTAQMGIPFLVSRSGLTHMGWQIAQKIGLTMIGRAQGKHYLLFSGADRFERD"
     assembly_gap    39386..39485
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <39486..39943
                     /locus_tag="PKN25_02320"
     CDS             <39486..39943
                     /locus_tag="PKN25_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HNV89761.1"
                     /translation="DAAGGAEAQLPPVESLSLSSDFTAFLKEEVSEALRRKALQKLFS
                     DPHFNRMDGLDIYIDDYSQPDPIPPEILAKLQHAREWLMDDATALPAPEGATAPVDGE
                     RGRGTTAEPATAVECASAQAATAPGGRCVDDLSGAVSSEPPQESGQNAP"
     gene            40072..42192
                     /locus_tag="PKN25_02325"
     CDS             40072..42192
                     /locus_tag="PKN25_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004384343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV89762.1"
                     /translation="MSERRDPTSRIHLCDCNRSFALDAGRLAAGTDAAMQCHHALCGA
                     ELPALQTSLAGGKTVHVACTQETALFGELAEEAGAGERIRFFNLREHAGWSAESAAAT
                     PKLAALIAAATTLADPEPVAAVQMSAGRSLLIIGEAGAALGWAERLAGSFEPAVLITA
                     RSKDVELPAVNAYPVWSGRPVRLGGHLGAFELEWTQHNPIDLELCVRCNACVKACPEG
                     AIGWDLQVDVDACRSHGACVAACGEIGAIDFARQDSARSERFDLVLDLSAEPLLRRVE
                     LPDGYAAPGRDPFEQALAVQALGEFVGEFEKPRYVAFEAALCAHSRAKKTGCSNCIES
                     CSTAAIRSNGDVIAVDPYLCKGCGTCSTVCPSGALSFQYPRVPELGQRVKTLLAEYAR
                     AGGTDACLLFHSAAAGSAAIARLARRGRGLPARVIPVEVWSADAVGLDLMLGSLALGA
                     CQVAVLAAGSHDAAPLEAQAGHGQAILSALGYGGEHLRVIEADADDWQALERALYDWA
                     PAMTVAEPARFRLLAKKRETLDFALRHLVAHAPATTTPAGLPAAIALNAGAPFGQVIA
                     SDACTLCMSCTGACPAGALRAASDAYRLEFVEKNCLQCGLCEASCPESAITLEPRLLP
                     GEYGTEGARRARALREADIFHCTACGKAMGAAPLIESMIARLSGHAMFAGEAERARLR
                     MCADCRVIDMMKAETAVKAWDLTE"
     gene            42203..42850
                     /locus_tag="PKN25_02330"
     CDS             42203..42850
                     /locus_tag="PKN25_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNV89763.1"
                     /translation="MSAAANASISIQPQPTWSDEDRARAEHYALIARLFHAAPDAALL
                     AALAQAGRTLGGEGGELPRAWAALGAAAQTMPLQAVAEEFDALFVSVGKPEVMCNGSW
                     YLTGFLQEEPLAELRDDLAELGLGRRAGVTETEDHIAALAEVMRHLVLTGPDDAGLAR
                     QRRFFGRHLAPWYAQFAGALAAAPGADFYAKVGGLLAAFMDIEREAFDMLEAEAR"
     gene            42918..43124
                     /locus_tag="PKN25_02335"
     CDS             42918..43124
                     /locus_tag="PKN25_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNV89764.1"
                     /translation="MKDENTKLSRRNFLAAIGAGSAAGAATLAATAAQASPVTAKVVE
                     EARREVADQGVSEHMRNYYRTTRI"
     gene            43138..>45039
                     /locus_tag="PKN25_02340"
     CDS             43138..>45039
                     /locus_tag="PKN25_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV89765.1"
                     /translation="MLTKKSATAATSARRLRGAAARQLGQTMDRRAFLKRSGLGVGAG
                     ALATQLPYNFIGAADAAAPAGVSRAEGKAEIRRTVCTHCSVGCAADAVVRNGVWVHQE
                     PVFDSPINLGSHCAKGAALREHGHGEYRLKYPMKLVDGKYQKISWEQALNEVGDRLLK
                     IREESGPDAVYFIGSSKHNNEQAYLLRKFVSFWGTNNTDHQARICHSTTVAGVANTWG
                     YGAMTNSYNDMQNAKAMLFIGSNAAEAHPVSLLHILHAKENGAKMIVVDPRFTRTAAK
                     AHQYIRIRSGTDVPFLFGLLYHIFQNGWEDKQYIDDRVFGMDKIREEVLAKWTPDKVE
                     EACGVPAEQMLLAARTMAENRPSTIVWCMGQTQHSIGNAMVRASCILQLALGNIGKSG
                     GGANIFRGHDNVQGATDVGPNPDSLPGYYGLATGSWKHWANVWGVDYEWVKGRYVSQE
                     LMEKSGITVSRWIDGVLENKDLIDQPAGNLRAVVYWGHAPNSQTRGAEMVEAMKKLDT
                     LVVIDPYPSATAAMAAMVRKDGVYLLPACTQFETHGSATASNRSIQWREKVIEPLFES
                     KPDHTIMYAFAKKFGWAEQFVKNIKLEKDAQGWDEPSIEDTLREINRGTWTIGYTGQS
                     PERLKLHMKN"
BASE COUNT         6362 a        15414 c        16674 g         6489 t
ORIGIN      
        1 agcctcgcca tcaccggaga ctgtacctgc cagtggtgcc agtacaggac cacctcttcg
       61 cgctcgggtg ccaggtcgac cagctcgccg cgcgcaaggt gcgcacgtgc ctggatctcg
      121 gggacgaatc catagcccag gccggcgagc gcgaaggcca tgaagccctc ggacgagggc
      181 acgacgtggt gtggatagcg ccgcgaatcc agcccgaagt ggcgcagcag gaaggcctcg
      241 tgcatgtcgt cgtggtggcc gaagacgatc gccggcgcgc gcgccagcgc cgccggggtg
      301 agaccctgcg ggaaccagcg cgcgcggaag tcgggcgagc cggcgcacag atagggcatg
      361 gccccgaggc gtacggcggc gcagccgcgc atcggatcgg cgcgggtgga cacgcaaccc
      421 agcacctcgc cgtcggcgag cagcgcgtgg gtgtggtcct ggtcgtcgac cacgcagtcg
      481 agcacgatgc cctcgtcgcg gaccacctcg gcgaccgcgc cgatgaacca ggtggcgagc
      541 gaatcggcgt tgacgcccac tgcgagacgc agcggcccgt ccacctcgcg tggcgacagc
      601 gcgcgtcgcg cctccgcctc catcaggcgc agctgtgcaa ggtagggcag cagcctgcgc
      661 ccggccggcg tgggccgcac cggcttgctg cgcaccagca gaacctggcc gagctcggcc
      721 tcgagcagct tgacccgctg cgacaccgcg gactgggtca gcgccagccg gcgcgccgcg
      781 cgctcgaatc cgccctcctg cagcaccgcc tcgaaggcca gcaagcgatg atcgatcatt
      841 agcgttattg ctcgaaaacg aaataaatcg atcctactta ataaaagcct gcctggcgcc
      901 tatattcgcg cctcccggcg cttctgcagg tccgcctcat gccggttctt gccggagcgc
      961 ccgcagtcgc gaacagggct gcggccacgg caacacgaga cgacaacgga acgcatcatg
     1021 agcaacgtct acctcaacgg ttttctcctc accctcggcc tgatcatggc gatcggggcg
     1081 cagaacgcgc acgtgctgcg ccagggcctg cgcggcgagc acgtgctgct gacgatcggc
     1141 gtgtcggcgg cgtgcgacat tctgttgatc ggcctgggta tcgccggcgt gggcagcctg
     1201 ttcgtcgcca accccgacct gatgacggtg gcgcgctacg gtggggcggc cttcctcgcc
     1261 tggtacggcg cgcgcgcgct acgctccgcg ctgcgcggtg ggcgcgcgct ggacctggcc
     1321 gagcacaagc gcatgtcgcg cggccaggcc ctgctcgcgg ccgccggctt cagcctgctc
     1381 aacccgcatg cctatctcga caccgtggtg ctgctcggtt cgatcggcag ccagcaggcc
     1441 gaggacctgc gcccgctctt cgccgccggc gcgatcagcg cctcgatcgt gtggttcctg
     1501 ctgctcggct acggcgcccg cctgctcgcg ccgctgttcg ccaagccggt ggcgtggcgt
     1561 gtgctcgatg cgctggtggc gctgatgctg tgggcgatcg cgggctcgct gctgctgggg
     1621 tgagcgggcg tgagcgccca ttcgcgcctt gcggtgctcc tacggaagcg accgcgtttc
     1681 gggaccccct gtaggagcgc cccaaggcgc gaacacagcg cccggtcgac cgcagcgcgc
     1741 attccacgcg tcggtttcgt cctgaaacgg atcgttcggg gggcggagcg tgtgaacccg
     1801 gcccgctata atcgatgccc tcgcgcatct cgcgcgcacc gatcaggatc tccgccgtgt
     1861 ccgaacgcct cgccgtcgcc ctccagtacg ccctgcccaa gcgcctgctc acccagcttg
     1921 ccggaaagct cgccggcctg gaggggggca aggcgacgac cgcggtgatc cgctggttcg
     1981 cgaagcgcta cggggtgaat atggccgagg ccgcgaacgc cgatccggcg agctacgcga
     2041 gcttcaacca gttcttcacc cgcccgctgc gcgaaggcgt gcggccgatc gccgatgcgg
     2101 acttcgtctg cccggtcgac ggggcgatca gtcagttcgg gccgatcgag ttcgaccagg
     2161 tcttccaggc caagggccac ttctattcca ccacggcgct cgtgggcggc gaccgggcgc
     2221 tcgccgcgcg cttcgagaac ggcgacttcg ccacgatcta cctgtcgccg cgcgactacc
     2281 accgcatcca catgccctgc gccgggcggc tgacgcagat gatccacgtg ccgggcgaac
     2341 tgttctcggt gaatcccgcc accgcgcgcg gcgtgcccgg tctgttcgcg cgcaacgagc
     2401 gcgtggtgtg cgtgttcgag ggcgaattcg gccccttcgt gatggtgctg gtgggcgcga
     2461 ccatcgtggg cagcatggcc accgtgtggc acggcgtggt caatccgccg cgccggccgg
     2521 acatcgtcaa gcgcggctat gcagagggcg aggtggtgct ggagaagggc gccgagatgg
     2581 gccgcttcct gctcggctcg acggtggtga tgctgttccc gcaggacgtg ctgcgcttcg
     2641 gctccgactg ggcgccgggc ggcccggtgc ggatgggcga gccgatggcg cgtgcgaagt
     2701 cgggcgcgcc ctccgatggt gcgccagcac tcgccgatgc accgtgatgg tgtacgggag
     2761 tggctccggc gggcgttccc ggatgtcatc ctgcggtcac gatgctgaaa gaatcccatg
     2821 ctagaacagc ggcttggcgt tccgtccgga acgcccggcg ccgacctccg aagctggcac
     2881 gattgaagct agggatgggg tgccgtccaa caacgagcat caggagacag atcatgccca
     2941 gccgtccgat ccatcagatc ctttccgatc gtcccttcgt cggcgtgtcg gccgacaccc
     3001 cggtgcgcga ggtggtccgc cagatgaacg ccctgcagcg cagcgccgcc ctggtcaccg
     3061 agcacggcgt gctcaccggc atcttcaccg agcgcgatgc cgccttcggc gtgctggccg
     3121 ccggcctgga cgccgacacc acgccggtgg gtgcggtgat gacgcacaat ccggtgacgc
     3181 tgaccgagga ccgccccttt ggtcacgccc tgcacctgat gtacgagaac ggcgtgcgcc
     3241 acgtgccgat cgtcgacgcc aaccgccgcc cgctcggtgt cgtcaccgcg cgcgatgcgc
     3301 ttcagctcga tgcgctcacc ttcggcgccg agctggtgcg gcgcgaggag atcacggtca
     3361 tcctgtaggc gccgtgttcg cggcttgcgc cgctcctaca gggggccgga aacgcggcgg
     3421 ctcttgtagg agcgccgcaa ggcgcgaatg cagcggtcga gatccaccca cgcgcatcgc
     3481 cttgccggac cgcctatcgc gcctgcccgc agcctccccc tcaaagcagg ttcatcttcc
     3541 gcgccgcatc gcgcagttcg ccacggaagt ccgggtgcgc gatgccgatc agcgcctcgg
     3601 cgcgctgttt cgcggtcttg ccgcgcagct gcgccacgcc gtactcggtg accacgtagt
     3661 tgatgtcgtt cttgctggtg gtgacgtggg tgcccggcga cagcaccggc gcgatgcgcg
     3721 acaccgtgcc cttctttgcc gttgacggca gcacgatgaa ggccttgccg tccttcgagc
     3781 ggttggccgc gcgcacgaaa tccgcctggc cgccggtgcc cgagtagggc aggtggccca
     3841 ggctctccga gccgcactgg ccgatcaggt cgatctgcat ggtggcgttg atcgcgcaca
     3901 gcttgtcgtt gcgtgcggcc aggtaggggt cgttggtgaa ctccaccggg tgcatctcga
     3961 tgcccggatt gcggtgcatg gtgcgataca ggcgccgcga gccgagcgcg aaggtggcga
     4021 tcgtacggcc cggcatgaag gtcttcttgc ggttggtgac cgcgccggac tcgatcagcg
     4081 acaggattcc gtcgccgagc atctcggtgt ggatgccgag ctcgcgcttg tgctgcaact
     4141 gcaccaccac cgcgtcgggg atgccgccgt agccgatctg cagtgtggag ccgtcgtcga
     4201 tgagctcggc gacgtacttg ccgatcgcct gctgcaccgg gccgatctcg ggcaggccga
     4261 cctcgaagag ttcgtcgtcg ctctccacca ggccggcgac ccgggagatg tgcaccaggc
     4321 agtcgccgtt ggcgaagggc acgttcggat tcacctcgag cagcacgacg cgcgcgcgcc
     4381 ggatcgccgc catggtgtag tcgggcgcga gcgagaggct gaagtagccg tgctcgtcca
     4441 tcggcgaggc cagcgacacc accacgtccg ccggtgacag gccgcgctcg atcagcatcg
     4501 gcagctcgga gaagtaggcc ggcacgtagt ccacccagcc ggcctggccg cccgcgcggt
     4561 tcgcaccgcc gaagaagtag gcgtcgtggc gcacgttcgc gcgcgtttcc gggtcgagat
     4621 aggcgaactt gcgcagcggc aggatctggc tcaccgcgac gtcgcgcagc gcgtgacggc
     4681 gcgcggacag ggcgtgcagc agcgccggcg gctcgcccac gccggtgggc acgaccacgg
     4741 tgtcgccgtc gcggacctgg ccggcggcgt cggaggcggt catgcgcttg ctgtcgtaga
     4801 gggcctgcag cttggacatc gcggttctcc cgggcgtgtg gtctggcgtc gaaggaagcc
     4861 gattatacgg gtattccata cggagtcgta tggtaaaggg tgcgcgctca agcaggtggg
     4921 tgggcgaggg cggcgcggat gtagtcctcg ggcaggcggt attcggcggc cagctcccgg
     4981 gcgttgcgcc cgctggcgcg gatcgtcgcc agccagccgt cgagcccgct cgacagcgag
     5041 cgcccggcct gttcgcccgc gcgggtggcg cgctcgctgc cgtcgaccgc gtacttgttg
     5101 gcgtagccgc gcggggtcac catcaacccg tcgcgcacga aggtgttgga gttgccgatc
     5161 agcaccgtgg tcagcatgcc gatgtcggcc tcggccatgc gctcgagggt gctgaactcg
     5221 atgcgctgcc tgggccggta ggcggacttg acgatcgcca ccggcgtggc cgggtcgcga
     5281 tggcgcagga agatgcgctg cgcctcgacg atctgcccgg tgcggcggcc gctcttgggg
     5341 ttgtagagcg cgaccacgaa gtcggcatag gcggcggcgt cgagccggcg tgcgatggcc
     5401 ggccagggcg tgagcaggtc ggacagcgag atcgcgcaga aatcgtgcgt cagcggcgcg
     5461 ccgacgagcg cggcgcaggt gttgagggcc gaggcgccgg gcacgatctc gacctcgatg
     5521 tcgccgggct ttccgctcgg cggtgtccac ccggcctgga agagcacctc gaaggtcggg
     5581 cccgccatgc cgtacacccc ggcgtcgccc gaggagacga gcgccacctt gcggccctgg
     5641 cgggcgcgct cgagcgcctc gatggcgcgg tcgagctcct cggtcatcga cttgcggatg
     5701 acctccttgc cctcgagcag gtcggccacg aggcggatgt aggtgacgta gccgatgatc
     5761 gtgtcggcct cggcgatcgc ggcgcgcgcg cgctccgtga ggtgggcgtg cgcgcccggg
     5821 ccgaggccga cgagcatgat cttgccggcg ggggcggacg ttccgggagg gacgatggtt
     5881 tcgggggcgt tcatgcggga gggactccgg tgcgaaacgc ggcccgcgag ggcgcgcgtc
     5941 gagccccggc ggtggtgccg ggtgctcggg gtggtcgtca gttcggggcg ggcgaggagg
     6001 cgggaaggcg ggcgatggag acggtggcgt tgcggccgtc cgggccgcgc agcttgtgct
     6061 tttcgacgcg cagcgcgccc gggtcggtgg cgagtgcggt gccggcggcg agcaaggctg
     6121 cggcctcgga gaccgagggc gtgccggtgt atcggcgcac cgtctccgac gggttgggca
     6181 cgtcgaccgc ggcgagcacg gccggtgggt agaaccgcag cgtccagccg gcggccgtgg
     6241 ccgcctccag caggccggct tcgtcggcct tcagcgtgat gctggccacg gcggcgacgt
     6301 cgatgcgcga cgcaccgatg cgggcgagcg ccgcgtcgat cgctcgcgtg atggtcgccg
     6361 ccggcgtgcc gcggtcgcag ccgaggccga gcgcgagctt cttcgccgca gcgatggggc
     6421 ggtagatcac ccgcttgccg gcgaggcgtg cagcgtagcc ggtgggcaga gggcggtcgc
     6481 tgatccacag cacggcgccg aagtgttcgg gctcgacgtc ttccaggcgc gcgaagcgcc
     6541 ggatgttcgc cggcagcggg ccgctgcggc cgttggcgtg gcgcgaccac cagtcggtgc
     6601 tgccggtctc ctgcaccagc gccaccggct cgtcgttcac caccgcggcg ctggcgcgca
     6661 cgatctcgtc gtggctggct tcgaaggtcc agccgagctc gcggccgagc aggtccaccg
     6721 ccagcgtcgc gcgcgcgtcc gatgcggtgg tcagcaccgg cgtggcgccc accgcctgct
     6781 gcagcgcggc ggcgatggcg ttggcgccgc cgaggtggcc ggacagcacc gggatcgccc
     6841 agcgcccggc ctcgtcgagc accaccacgc cggggtcggt ctccttgttg ccgagatgcg
     6901 gcgcgatcag gcgcaccacc gcgccgagcg acacgatggc gacgatgccg tcgaagccgg
     6961 cgaacagcgc cggcacctgg tcgcccacct tgccctcgta gcaggcgaag gcgccgggtg
     7021 cggctgcggc ggcctcggcg gcgaacttcc gcggtgcgaa cagcgtggcg ccgggcaagg
     7081 cggcgatgac gcgaccggcg agcgcgatgc cgtggcgggt gatcgccacc acggcgatgc
     7141 gggtgcaggg gaagggcaga ggctggaggg gctgcgaagg catcgggcgg aacctcagga
     7201 aagggccgtg gccgcggggg actcgccagg gtgcgcggcg gcgcctccgg tgtccgggga
     7261 gggggcctgc gcggtggctt gcggttccac ttgcgcggcg cgcttgcggc agccgcggcg
     7321 cagctcgccg cgcgcgcgct tggggttctg caccagcagc agggagaggt aattgacctg
     7381 cgcgccgcgc aggctggcaa ggtcgtgcac cacgcgctgc gcgggcgccc ccaccttctc
     7441 gacgaagcag ctcgtggcga gcaggccgcg gcgctcgagc aggtcgagca cctcgtcgag
     7501 cagcggcttc accttcatca gcaccagggt gtcgaactcg tcgagcaggt ggtcgatgac
     7561 ggtggtgccg taggcggcgg ggatcaccgc catcgtctcg tcctcttcgg ccagcgccag
     7621 gtcggccacc gccgcggcgg cggcgaagga actgaccccg gggatggtgt cgacctcgac
     7681 ctcgggcgcg agctcgcgca ccgtgcgcgc gaggtggcgg aaggtggaat aggtggaggc
     7741 gtcgccctcg accaggaagg cgaggtcgcg gccttccgcg agcagcgcca ccacctgcgt
     7801 cgccgcgcgc gcccaggcct tggcgagtgc gacggcgtcg cgcgtcatcg gaaagacgag
     7861 ctccaccgcg tccgcgggga tggacagacc gccgcggcgg acgatgtcga gcgcgtagga
     7921 ggattcctcc gccttcttca ccggatacgc ccagcgtgcg tccgactgca gcgcggccca
     7981 gccgcggcgg gtgacgagct cggggtcgcc ggggccgagc gagatgccga tcaggcggcc
     8041 gaagcgagcc gtgttcgcgg gatgcggatt catggagcct ccttgcgggt ggtgacgatc
     8101 cacaccgggt tctgcgcggc gagccgatgc atgtcgagga tgggttggct gcgcgcggcc
     8161 gagagcatcg tcacctccca ggcggtgtcg gccgcggcca gcacggaggt ggcggtggcg
     8221 aggttctcga gggtgacgaa gttcatcacc agccggccgc cgggaacgag ccgccgcagg
     8281 acgagggtga tcagctcgcc gagttcgccg cccgagccgc cgatgaacac cgcatcgggg
     8341 tcgggccagg catccaggcc ggcgggggcc ttgccctcga acaggctgta gttgctggcg
     8401 cgcagccgac gggcattggt gcgtgcattg gcggcgtcgg cggggttctt ctcgatcgcc
     8461 catacgtggc cgtggcgggc gatgcggctg gcctccagtc cgaccgagcc ggaaccggcg
     8521 ccgatgtccc acacccggct gtcggcgcgc agcgcgagct tggccagcgc gaccgcgcgt
     8581 gcctcgagct tggtgatcag gcccttctcg ggtgtgcgct gcacatagtc gaggtcgtcg
     8641 acgccgaaca gcggggtacc gatgcacgtg cgctcgatca ccacgacgtt gggttccggg
     8701 aagcgccggc tggcggcctc ctgcaggctc agcccggcga acaccgcttc gtcgtccagg
     8761 cacaggcggg cgaccaccga caggcgcagg tcctcgtcgc agcccggcgc gccatgacca
     8821 gccgcgagca aggcgcgggc gatgcggtcg gggctgttgg cggggctggt gaagcaggcg
     8881 accagcgggt gttcggcgac ggcgcggacc acgcggtaca ggccgtgctc cggcgtgggg
     8941 ccgggttgcc gcggatcggc ctgccattcg tcggcgtcgg cgccgtggca agacacgaag
     9001 cgggcctcct gccacgacct cttcaggcgc gcgaaggcga gctgcagcgt cgaggttgcg
     9061 ggcagtacct cgagccgcgc ggcgccgagc ttgtcgatca ggaagccggc gatgccatgg
     9121 cacaaggggt cgccggtggc gagcaccacg gcggcgtcgc cctcgagcac ggcctcctcg
     9181 atccagcccg gcacgcgtgt gagcgcgccg tccatgttca gcaggcgggc gctgggggcg
     9241 tgcggtgcga tgcgttccag cgtgcgtccg gcgccgatga ggctggcggc ggcttcgatg
     9301 cgccggcgcg ctgcggcgcc gaggccgtcc cagccgtcgt cgagcaggcc gacgatgctg
     9361 cagacggggg cagtggattc aggcatgcgt ggtctccttc aggtcggcct catcgacgct
     9421 cacgatcggc acgccctcga agttgcacgc cagcacggtg agccggtgcg ggccggggta
     9481 gcgctggcgc aggctgcgga tggcgcgccc ggcgagcgcg cggtggaagg ccacggccag
     9541 gccgagcgcg ctcaggcgtt cggcggcgaa gcgggcggtc tcggcggcgc ggatctcggc
     9601 cacgagcgcg ggcggcgcgc cgacctcggc agcggcggcg gcgatcagct cgcgatcgac
     9661 ctcctcgcgc caggcgtggg tgaccgacag gccctgggcg atcttggtga gcttgccgac
     9721 catggcgccg acgacgatgt ggcgcatgcc ctgcctcacc gcggtggtga aggcggcctt
     9781 gacgaagtcg cccatctgca cgaagcaggc ctcgtcgagg tccgggaagg cgcgcatcgc
     9841 gcatttctcg gtgcgcccac cggtggtgaa caccacgcag gtctggccct ggttggcggc
     9901 gacatcgatg gcctggatca cgctggcgcg gaaggcggcg gtggaatacg ggcggacgat
     9961 gccggtggtg ccgaggatgc tgatgccgcc gaggatgccg aggcgggcat tgagcgtctt
    10021 ccgcgcaatc tcctcgccgc cgggcaccga gatcgtcacc tccagaccgt cgccggcctc
    10081 gaggatgggc gtgccggccg cgcgcacgtt gtcgatgatg ttgcgccgcg gcacggggtt
    10141 gatcgcgggg ccgccgaccg ccagcccgag cccgggcttg gtgaccacgc ccacgccagg
    10201 gccgcccgcg aggatcacca cgccgccgcc cccgcggatg cggcgcacgt cggcggtgag
    10261 acgggcgcca tgggtggcat cgggatcgtc gccggcgtcc ttgatcgaga ccgcgtgggc
    10321 gtggtcgccg tccacctggc cgtcgcggat cgtgaagcgc acccgcgtcg tgttgggcag
    10381 cacgcactcc accgcgtcgg gcaccgcgcc gcgcaccagg ccgagcgtgg cggccgccgc
    10441 ggccgcggcc gagttggcgc cggtggtgag gccggtacga ttgccgcgcg cgcgcctggc
    10501 gtcgcccttg cgcaccttgt cggggaggga atgtcctgcc gccatcgtgt cgcttcagcc
    10561 ccggacggcc tgtcgctgcc gtgtctcggc cagcccgagc agggcgtgga tcgccgccac
    10621 caccagggtg gagccgccct tgcggccgcg gatgacgatc cacggcaccg cgtcgaccgc
    10681 ggccatccgg tccttcgatt cggccgccga cacgaagccc accggcatgc cgaccaccag
    10741 cgccgggcgc acgccctcgt cgcggatcag tcgcaccagc tcgatcagcg cggtgggtgc
    10801 gttgccgatg ccgacgatgg cgccgtcgag caggccgagg cggtgcgcct tgcgcatggc
    10861 ctgcaccgcg cgcgtgctgt cctcggcgcg ggcgtgctcg acgacgtcgg ggtcggagat
    10921 gaactggtgc gtgcgcatgc cgaagtgggc gagacgcgag gcggacaggc cgacacagat
    10981 catctcgacg tcggcgacga tccgcgcgcc gccgccgagg atggcggcca ggccggcgtc
    11041 caccgcatcg ggatggaatt cggtgaggcc gttgaactcg aagtcggcgt tggcgtggat
    11101 catgcggcgc accaccggcc actgcccggc ggtgtaggcg tgacggccga cctcggcgtc
    11161 gatgatggcg aaggagtcgt gctcgatcgc gcgcccggcg gcggtgagct gttcggtgac
    11221 ggtgttggtg cgcatggcgg gttcggcttc aggcgtgcgc gtggccatgg gcgcgagcgt
    11281 atgcatggcc atggtcgtgc tcgtggtgcg caggggcgtg acgggcgtgc gtggggctgt
    11341 gctcgtgcag gtgcacggcc tcggcggcga ggcggtatct gcagccgtcg cactccagca
    11401 gtccgccttc gggtgcttcg tggtcgtcga ccctgcggtc gagcagcgcg aagatgccct
    11461 cgtcgaagcc gaagtgggtg ccgagcgcga aggcgagctg cgggtactgg cgccgcaggc
    11521 gctcgacctg tgcccggatg cgctccatca gcaccccggt gaagaggtac accggcacga
    11581 tgcagatctg cgtcatgccc aggcgggcct ggcgctgcac cacggtctcg agtcgcggcc
    11641 aggtcacgcc ggtgaaggcg aggtcgacca gctcgtggtc gccgtcctcg aagagccagc
    11701 gcgccatctt ggcgaactcg ccgttggcgc cggcgtccga ggagccgcgg ccgagcagca
    11761 ccacgccggt ggtgtgcggg tcgggcatgt cgagctgcct cagcaggcgg tcgagctggc
    11821 cgcgcagtac ggcgaagagc tcgcgcccca tgccgaggtg gcgggcgcag gcgaaggcga
    11881 cgccggggtg gcgggcgcgg gcgcgctcga tcgcagccgg aagctccatc ttgacgtggc
    11941 ccgcggcgtt gaggatgaag ggaatcgtga cgacacggtg cgccgcgtgc gcggcgcggt
    12001 cgaggccctc gtcgagcagc acgtcggcgt gctcgatgaa gcaggtctcg atgcgccagc
    12061 cggggtggcg ttcgcgccat tgtgcggcga agtggaggat ctccttgttg ccctcgcggt
    12121 tgcgcgagcc gtggccgacg agaaggatgg cggtcttgtt catgcggttt ctccggtggc
    12181 gatgccggcc gcggcggccg gcatcgacgc cttgcggaag cggtggccga agcccgggtc
    12241 gtagagtttc gagcgtgcga ggtcgggcca caggcgggcg ccgagcgccg gactggcgag
    12301 aatcatggcc tggctggcga tcttctcgtc ctggcaacgg cgcttgatgt cggccagcgt
    12361 gccgcgcacg atcttctccg cgcccgccca gctcgccttg tgcaccacca cgatgggggc
    12421 gtcctccggc cagccggcgg cgcgcagcgc acgctgcacc tcgtgcagca gcgtgatcga
    12481 caggaagatg cacagcgtcg tgcggtgcgc ggcgagcgcc tcgagcgcct cgcccggcgg
    12541 catcggcgtg cgcccggcga cacgggtgag gatcaccgtc tgcgtcacct ccggcagggt
    12601 cagggtctcg cccgcggcgg cggccgcggc catcgccgag gacacgcccg gcaccacccg
    12661 ccaaggaatg ccggcggcgt cgagcgggcg cgtcatctcg accagggcac cgtacaggcc
    12721 ggggtcgccg gtctgcaggc gcaccacgac gggatggcgg gcgcaggcgt ccagcagcca
    12781 ggcgctcatg ccctcgagcg tcatgtccct ggagtcgcgg atctcgcagt gggcgggcgc
    12841 gaactgcgtc gcgctgcggt cgaccagcga gccggcgaac aggatcgcgc ccgcccgctc
    12901 gagcaggcgg cggcctttca cggtgatcag gtcggggtcg ccggggcccg cgccgacgaa
    12961 ccagaccgta cccggcagcg cggtcatcag ccgcgcgccg gcacggcgaa gcagagccgc
    13021 acgaggggac gatgcgcctg cgactgcagc aggcggacga gtgcgaccgt cagcaccggc
    13081 tcgatcagcg ccaccgggag gtaggcgctg gcgaaggcgg cccatgccgc gaagggcgtg
    13141 gcgacctcgc ccatggccag ccagaagccg accatgacgg cgacgccggc gtagtaggcg
    13201 gcgtcgagct tcagcaccga tcccacccgc gtcgcggcgc cgctggcgag cctgcggccg
    13261 agcgtggcat gcagcgccac cagcggtacg gccagcgaga ggaaattgac cccgagatgc
    13321 agcaggtcgg cgggctcgaa gaccccgccc tgaagcagca ggccgaggcc gaagccgagc
    13381 agggtgggca cgaagccgag cgtcaggtag atcggcatgg cgccgatgaa gtgcagctcc
    13441 gatgcgccgg cgggcgtatg gaagctctgc atgaagacgg agaagaacag ggctgcgagc
    13501 agggtgcgca gcaagcgctg cggcttcctg agaagttgcg gcgcctgggc ggcgacgagc
    13561 gcggtggcgg cgaggttggc ggcggcgatc ttggcgccgg aaaggatgcc gggttcgatg
    13621 tgcatgatga ctccccacga gtgcatggaa cgacgatgcg aaccacgcgg gcggggggcg
    13681 gcatgcgggc gcggcgctgc cggtgggcgt acgcacgcgg tgaaaccgcg agcgcacgcc
    13741 gggcacggca ttgcctggag accgcttgca cgacgagtcg gctggagctt ccggcgcaca
    13801 cgggcagccg gcggaaaacc agtcggatcg aacccacacc ccggggctca catcaacctg
    13861 gaaccgcgca cgacgccact tgcaggcggg tgcgggttcc gtactcgatc gggccggtct
    13921 tctggcttgc cgtcttcctc ctccgccggc cttcccgagc gcaaggctca gtggcgtgtt
    13981 ggcggattcg tcaggctcac agcagcgggg gctgcgccgg aatggtcgtg cgcgcgcaag
    14041 gcggacgacg tcaccggact tcccgtttca ccctgtcata tgaagttatg acagggcacc
    14101 cggatcgaat gcggagcgaa ggataggcga agctggggcg ggaaggcaag cgatgtgcag
    14161 cggtggggac gggagcgccg cggtcgcgat gccggttttc gtgggagcga caccagtcgc
    14221 gatgcggcca tcggcgcggg ttatgcgaat ctcggacccg cgccgacggc ctcgctgttt
    14281 ccggtcgtcc tgctcagcgc ttgtcgccgc ccggcttggc ggcgaacagg gcggcgcggg
    14341 gctgcgggcg gcgctgcggg ttcgcgccgg cggggcgggg cgcgttaccc tccgggcggc
    14401 ggttggcgga cggtgcgcgg ttgccgtcca cctcgacacg gcgggccggc acggcgcggt
    14461 tgccatcgac gtccgggcgc ggagccgagc cgcggttgcc gttgggctcg cgcgcctcgc
    14521 ccgtgcgcgc cgcgcgattg ccgtcggcct cgccgcgggt gcgcgcgggg gcacgtccgc
    14581 cgggctgggc cggagcacgt ccgccgcctg cgcgctcgct gcgcgcgggc gcggcggcgg
    14641 ggctgcggcc ctggccgttg cggccgccgc ggcgctgcag cggttcgcgc tggtggtcgg
    14701 cgtcgtgtgc ggcctcgccg ccggcgctgg gcacgaagtc ctcggccacg gcgcgctcga
    14761 tgcggcgctt gatgacgcgc tcgatcgcgg tcaacagctt gatttcttcg ttgtcgacca
    14821 gcgagatcgc attgccggcg gcgccggcgc ggccggtgcg gccgatgcgg tgcacgtagt
    14881 cctcggcgac gttgggcagc tcgaagttca ccacctgggg cagctggtcg atgtccaggc
    14941 cacgcgcggc gatgtcggtg gccaccagca ccggcaggga gccgtccttg aactgggcca
    15001 gcgcgcgcgt acgcgcggcc tggctcttgt tgccgtggat cgcggcggcg ccgatgtcgt
    15061 gcttggtgag gtactcggcg agcttgttgg cgccgtgctt ggtgcgggtg aacaccagcg
    15121 cctggaacca ctgcttttcc ttgatcaggt gggcgagcag ttcgcgcttc tgcttctggc
    15181 cgatcaggta caccgcctgg tcgacgcgct ccgcggtggt gttgcgcggg gcgacttcca
    15241 cttctgccgg gttgtgcagc aggccgtggg cgagctcgcg gatctcgtcc gagaaggtgg
    15301 ccgagaacag caggttctgg cgcttcttcg gcagcagcgc gagcaccttg cggatgtcgc
    15361 ggatgaagcc catgtcgagc atgcggtcgg cttcgtccag caccaggatc tcgacgccgg
    15421 agagatcgag cgtcttctgg ccgacgtggt cgagcaggcg gccgggggtg gcgacgagga
    15481 tgtccacccg cttcttcagt gcgctgatct gcgggttgat gttcacgccg ccgaacatga
    15541 ccagcgagga cagcggcagg tgcttgccgt aggtgtcgac cgactcctgc acctgggcgg
    15601 cgagctcgcg cgtgggggtc aggatcaggc agcgcgggcg cccgggcggg tgcggatggg
    15661 tgtgggtctg cgacaggatg tgcagcagcg gcagcgtgaa gccggcggtc ttgccggtgc
    15721 cggtctgcgc cgccgcgagc aggtcgccgc cggcgagcac ctgcgggatg gccttggcct
    15781 ggatgggcgt gggcgtggag taacccgcct cggagaccgc gcgcaggatg ggttcggcca
    15841 gcccgaggct ggcgaaggtg acttggggat tcattcgatg gtccggcgca agcccgtcgc
    15901 atgcatcgat gcgacaccaa tccgggctgc gggagggatc cgcgccgcat gggggcgaac
    15961 ggatcgttgc aggaaacgcg gcgggtgact ggtgcaccgc agcgaagccg gcactataag
    16021 cgctccacac cggaatggcg agccatttcg gcccgctggc gaccgcgaat ccgcccccgc
    16081 ggcctcgggg gcgctggcga atcttgcgct ggatcgtgtc atggaatgaa ggtttggtta
    16141 caatccgaca caacccggtt cccggcaggg gtattcccgt gcgcgaaatc cagctctccg
    16201 aagacctcga cggccgctac gatcggctct cccgcgatct gggcgcgcgc gcgctcgccg
    16261 gcctgctcgc cgtgtccggt tcgatcctcg tcctgctcgg cggggcggtc gtgggtgccg
    16321 tggcgacctg ggaggcgatc ggccggggca caccggtgga aacgctgttc tgggtgctct
    16381 tcatgctcgg cagcatcgcc gcgatctggg ccacgctggc ctccctggcg agcctcgcgc
    16441 cgcggccctg cgggctggcg gtcgcgcgcg aacgggtggg cgagctctac gcgctggtcg
    16501 atgcgctcgc gcacgtggcg ggggtgagcc cgatccggcg catctacgtg acgggcgaga
    16561 tcaacgcctc gatcgtgcag cgcccgcgcc acctgggtgt cggcgagatg gcgaccgatc
    16621 tgctgatcgg cctgccgctg gcccatgcgc tcggcccccg ccagctcgcg gcggtgatcg
    16681 cccacgagat cggccacatg gtcgcacgcc agcgcgggat gcacggccgg agcggttggc
    16741 tgcaggcgtg gtggatgcgc acgctggacg agcttgcggc cgcgttgccg tccggctttc
    16801 cctggttcga cccccagggc gacgccctgt gcatgaggat gctgctgctg gcgcaaatgg
    16861 aggagtacgc cgccgaccgc accgcggtgc gcctggtagg ggccgagctg cttgccggga
    16921 ccctggtcga gctctgctgc aaggcggact tcctcgccaa cgactacctg ccgcgcgtgc
    16981 atgccctggc cgagtgcgac gaggcgtctt gcgtgcggcc ctaccgcgag atggggcacg
    17041 gctttgccgc cggcttcgcc caatcgagcg ctgccatcga tccgcagcgc gtgctgcgcg
    17101 cggacgccgg cgaccccttc caccccgcgc tgcaggatcg cctcgtggcg atcggcgtcg
    17161 ccatgcccga gcggctgcag gtgtcgggtc cttctgcggc gcagcatttc ttcggcgaca
    17221 gcctgccttt cctggcctgg catttcgacc ggatctggtt ggagagcctg cgcgacgctt
    17281 gcccgccggc gctcagtccg cgcgcgccgg agcgggtgct gcagggggcg tgagcgccgc
    17341 ggaggcgccg ccgcggcgct tgcgtgcggt ctcgacctgg ccgcacaggc gcgccgcgcc
    17401 gtcgaggatg cgcggcgtat ggcgctggat gagctcgggc gggatgaaga acaggttgtc
    17461 gcggcgcgcg gcctcgagct gcggccagcg cgaccactgg tcgagccact ccggacgggc
    17521 ctcgcccatg cccgaggcca cgatcacctc ggggttggcc gcgagcaccg cctcgacgcc
    17581 gatcgtcggc gtcaggtggg cgacctcgcc gaacacgttg gcgccgccac agaggcggat
    17641 cacgtcggcg atcaggtggt ggtcgttcac cgtcatcagc gggcgatccc agatctggta
    17701 gaaaacgcgc accttgtcgc gtgccgaata cgtggcggcg aggtgctcgc ggcgcgcgcg
    17761 gaaggcgctc gccgcggcct gggcggcggg ttcgctgccg gcgagctgcc cgagtcgctc
    17821 caggttgcgc gccacgtctt ccaggttgcg cggctcgctc aggtagaccg ggatgccgag
    17881 cgcctgcagg cgggcgagat cgccttcgcg gttgccgctc tgccagccga tcaccaggtc
    17941 cgggcgcagc gccgcgaccg cctcgaggtc gatccgggtg tagccgccca cacgcgcgat
    18001 gcgctgtgcc gcctcggggt agtcgctgta gtccaccgcg cccaccacgc gctcgcccgc
    18061 gccggcggcg aacagcatct cggtgacgtg cggggcgagg ctgacgatgc gctgcgccgg
    18121 cgcggccagc gcgagcttgc ggccggtgtc gtccaccagc tcgacgcccg cggcggacgc
    18181 ggggccgccg agggcgagca gcgtggccgc tgcggccgag gccgctgcat cggaaagacg
    18241 gcaggggaga gaactcatca cggctccttg aggagattcg ggcagtctgc gagggcggcc
    18301 tccagcctcg cccagcgagg ctcgtccgcg ggcaggccga agcgcagcgc gggcggggcg
    18361 tcgaagcggc gcagcaggat gccgcggcgg gcgaaggccg cgtgcacctg cgccgcgtgc
    18421 ggcaggcgga cgtactggaa caaggccggc ccgcagcttt ccagtccatg cgcctgcagc
    18481 aggcggtgca ggcgcgcgcc cgcggcgtcc agccgggcgc gctgcgcggc ctgccaggcg
    18541 gtgtcggcga gcgcgtggtg cgccgcccag cgtgccggac cggccagcgt ccacggcccg
    18601 aggcgttcgg cgagcgccgt gcggacctcg gtgggtgcga acacgaagcc cacgcgcgcc
    18661 ccggccagac cgaaaaactt gcccagcgag cgcagcacca ccaggccggg gcgaccggcg
    18721 agcggcacca ggctgtgcgc aggcgtggtg tcggcgaaag cctcgtccac caccagccag
    18781 ccgccgcggc gggcgagccg ggcatgccag tcgagcaggc gatcgcggtc gaaatgcacg
    18841 ccggtcgggt tgttgggctg caccagcacc accacgtcgc tgtcgtcgat cgcaccctcg
    18901 acctcctccg cgtcgatcga ccgtggccgg cgctcgcgcc aggcgtgggc gtgctcggcg
    18961 tagcctggcg ccagcaggcg gacgcgtgcg ccgggcagga ccgccggcag ggcctggatc
    19021 gccggctgag aacccgccac cgcgagcaat tcggcgctgc cgtaatagcg cgcggcggct
    19081 tcgtccaggc cgtcgtcgtc ctcgggcagg cgctgccagg ccgtctccgg caccggcggc
    19141 accggatagg cccacgggct gatcccggtg gacaggtcca tccagtcggc aagcgcgata
    19201 ccgtagtgct cggcggcgcg gcgcaggcgg ccgccgtgct caagcaccgc cgcctcccgc
    19261 cagcgtcgtc gcgacgccga ggaccgcggt cagccccagc cacagcagcg caccacgccg
    19321 caccagggct accgccgcgc ggatggcgtc gacgtcggcg ccacggccct cgcccagtgc
    19381 cgggcggtgc tccgcgcggc cgtggtaggt cgccgcgccg cccaggcgca cgccgagcgc
    19441 gcccgcaccc gccgccatca ccggcccggc gttcggactg tcccaggcgg gcgcctgcgc
    19501 gcgccagcac gcgagcgccc gccgggtgtc tccgagccag gcgtaggtga gcgcggtgag
    19561 gcgcgccggc agccagccga gcagatcgtc gatgcgtgcc gcagcccagc cgaagtgcag
    19621 gtggcgcggc gtgcggtagc cccacatcgc atcgagcgtg ttcgccaggc ggaacagcac
    19681 cacccccgcg ccgccaccga gcacgaacca cagcagcgcg ccgaacaccg cgtcgttgcc
    19741 gttctccagc accgactcgg tggccgcctt ggccacgccc tccgcgtcga gcgcgcgggt
    19801 gtcgcggctc acgatccagc ccacccgcgc gcgcgccgcg tcaaggtcgc cggcggcgag
    19861 cggcgtcgcc accgccagcg cgtgctcggc caggctgcgt ccgccgaggg cgaagtagag
    19921 cagcgcgctg tccacccccc agtgcgcgtg cggatggagc gtacgcagcc acagcgcgag
    19981 cgccacccaa ggcagcaccg cgagcgccca ggcgagcaat cctgccaggc gcatgccggt
    20041 ctcgttggtg ccgaagaaca gccggcggca ggcccgctcg acccagcccg cccagcgccc
    20101 gaagccgacc agcggatgga agcgcctcgc ctcgccgaat acgcgatcgg cgagcaggcc
    20161 ggcggccatc ttgagcgtga ggacgagcag caatgaagag ggcatcggaa cagtcaataa
    20221 aggaacgtgg gcgaatcacc ccaagcagcg aacacaaccg caagcaccac ggccccgccc
    20281 gcgccgggcc gggcagcctg cgcaagcccg ggcgcccacc gtaccctccc gaatcggcga
    20341 taatcccgcc ccttcgagca aaacccgcag gattctacgc catgtccccc ccgatcacgc
    20401 tgatggtgca aggcaccacc tccgacgccg gcaagaccac gctggtcgcc ggactcgccc
    20461 gcgtgctgcg ccgacggggc atcaaggtcg ccccgttcaa gccgcagaac atggcgctca
    20521 actccgccgt caccatcgac ggcggcgaga tcggccgcgc ccaggccctg caggcgctcg
    20581 ccgccggcgt cgccccgcat accgacttca atccggtgct gctcaagcct tccaccgaca
    20641 tcggcgccca ggtcgtcatc cacggccgcg ccgtcgccag cctgtcggcg cgcgactacc
    20701 acgcctacaa gcccaccgcg atggccgcgg tgatgcagag ctgggaccgc ctgtgcagcg
    20761 cctacgcggc ggtgctggtc gagggcgccg gcagcccggc cgagatcaac ctgcgcgacc
    20821 gcgacatcgc caacatgggt ttcgccgaag ccgccgacgt gccggtggtg ctggttgcgg
    20881 acatcgaccg cggcggcgtg ttcgcccacc tcgtcggcac cctcgagctg ctctcgccct
    20941 ccgagcaggc gcgcgtcaag ggcttcgtca tcaaccgctt ccgcggcgac ctcggcctgc
    21001 tgcagtccgg cctcgactgg ctggaggcgc gcacggggcg gccggtgttc ggcgtgctgc
    21061 cttacctgca cgggctcttc ctcgacgccg aggacgcgct cgccgacagc gccgccgacg
    21121 tgcgcgcggg cgaggctgcg ctgcgcgtga tcgcgccggt gtactcgcgc atctccaacc
    21181 acaccgacct cgatccgctg cgattgcacc cgcaggtgga tttccgctgg atcggccccg
    21241 ggcaggcgat cccgcctgcc gacctgatcg tgctgcccgg ctccaagagc gtgcatgccg
    21301 acctcgcctg gctgcgcgcg cagggctggg agacggcgat ccgccgccac ctgcgctatg
    21361 gcggcaagct gatcggcatc tgcggcggct tccagatgct cggccgcgag ctgtccgacc
    21421 cgctgggcct cgaaggcgcg ccgggaacga tggccgggct gggcctgctc gaggtggaca
    21481 cggtgctcga acaacacaag cagctgcgca acctgcgcgg ccgcctcgcg aacctgtcgc
    21541 gcgaagacgg gtccgagtgt gcaggtgaac ccggtgcggc cgtcaccggc tacgagatcc
    21601 acatgggcgt gagctccggc ccggcgctgg cgaggccggc gctgtggttg gcgcacggcg
    21661 acgaggccat gccgacccgg cccgacggcg cgcgctctgc ggacgggcag atcctcggca
    21721 cctacgtcca cggcctgttc gacgcccccg cggccttggc ggcgctgctc gcctgggcgg
    21781 ggctggacgc ggaggcgatg gaaggatccg ggccggtcga cctcgccgct cgccgcgaag
    21841 ccgatctcga ccgtctcgcc gacgcgatcg aggcccatgt cgatctggca tccctgttcg
    21901 ccccgaatcg cctctgaagt cttgcgggat ccgcacgaat gaacaccgac accccgcgcc
    21961 gccacggcgc cgaatcctcc ccgctcggtc aggcggtcgc ctaccgcgac acctacgcgc
    22021 ccgagctgct gttcccgatt tcgcgccagc tcaagcgcga cgagctcggc ctgcgcgccg
    22081 acatgctgcc cttctgcggc gaggacttgt ggaacgccta cgaactctcc tggctggacg
    22141 cgcgcggcaa gccggtggtg gccctcggcg agttccgcgt gccggcggct tcgccgcgac
    22201 tgatcgagtc caagtcgctc aagctctacc tgaactcgtt caaccagcag cgctgcgcga
    22261 gtgtcgagga agtgcgcgcg accatcgcgc gggacctttc ggcggcggcc ggcgccgcgg
    22321 tggcggtggt gttgcggccg ctgcaggaac gccctgggcg cagcttcggc taccccgagg
    22381 gcgtctgcat cgacgcgctc gagatcgaca tcgggcgcta ccagccctgc gcggagacgc
    22441 tgcgcgccgc gggaccggag gtggaggaga cgctgtactc gcacctgctc aagtcgaact
    22501 gcctggtcac cggccagccc gactggggta ccctggtcgt gcgctaccgc ggggcggcga
    22561 tcgaccgcga gggcctgctg cgctacgtgg tgtccttccg cggccacaac gagttccacg
    22621 agcagtgcgt ggagcgcgtg ttctgcgacc tcatggcgcg ctgcaagccg caggagcttg
    22681 cggtgtgggc gcgctacacc cgccgtggcg ggctggacat caatcccttc cgcgccagcc
    22741 gggcggggct gcgggcggac gaggccatgg aagtgcgcca gtgagcgcag gcgcggcaaa
    22801 tgtgcaacat cgtatgcggg ccctgcccaa accggcgcat gcggtggttt aatacctgcc
    22861 ggtaacaacc aacaccaaga ggtaaggaat atgaaggctc tcgttgctgc tgcggtcgct
    22921 gctggtctcc tctccgcttc gcccgctttc gcgtccgagg cgctggccaa gtccaagaac
    22981 tgcctggcct gtcacgcggt ggacaagaag ctggtcggcc cttcctacaa ggaagtcgcc
    23041 gccaagtacg ccggccaggc cgatgccgcg gcgatgctcg ccgacaaggt gcagaagggc
    23101 ggcgtgggcg cctggggcaa ggtcccgatg cctcccaacc cgcaggtcaa tgccgccgac
    23161 gccaaggcgc tcgtcgactg gatcctgacg ctcaagtaat gccgcattcc gggccggcgc
    23221 tgccggcccg cgtgcctccg aagccggcgc aagccggctt ttttacgccg gtgcggcctg
    23281 caggtccgcg ctgccggcgc cgaggaagag ccgttcgggc tggcgcagcc agcccggcac
    23341 ctcgccggcc ggggcggggc gggcgaacag gtagccctgc atcacctcgc agccgagcgc
    23401 gcgcagcgcg tcggcctgct cgagattctc cacgccttcg gcgacgaggt cgagatcgaa
    23461 gccgcgtgcg atgccggtga tggccgagat gatcgggttg gtcatcgggc cttcgaggtc
    23521 acgcacgaag ctgcggtcga tcttcagcgt gctgaccggg aagcgctgca ggtaggcgag
    23581 tgccgagtag ccggtgccga agtcgtcgat cgccacgctg atgccggcct cgcgcagcgc
    23641 gcgcaccttg gcggcgatgt cggcggtgtc gtccatcatc aggccctcgg tgatctccac
    23701 ctcgaggcac gacggtgcca cgccgtggcg gtcgaggcag gcgcggatcc tgccgaccac
    23761 gtcgccgcgc tggaagtcgc aggccgacag gttgatcgac acgcgcgggg cgccgacgcc
    23821 ctccgcctgc cagcgtgcga gctgcgcgca ggccgtctcc agtacccagt tgctgatctc
    23881 gctgatgagg ccgacctctt cggccacctg gatgaaggtc gcgggcggaa tcgcgcccag
    23941 gcccggatgg ttccagcgca acaaggcctc gaggccgacg atgccgcgcg tcgccaggct
    24001 gacctggggc tggtaatgca ggtcgagctc gccgcgggcg accgcgccgc gcaggtcgtt
    24061 ctccagcgcg atgcggtcgc gatggcgggt gttgagctcg gggtcgaaga agcggaaggc
    24121 gttcttgccg gagcgcttga cctggtacat cgccgcgtcg gcgtggcggg tgaggtcctc
    24181 ggcagtggtg ccgtcgcgcg ggaagagcgc gatgccgacg ctgaccgtgg cacggaattc
    24241 gccctggctg agccgcaccg gggtggcgag tgcgtcgagc accttgcgcg cgatgacctc
    24301 ggcgtcctcg ggctggttga tgtccgggag cagcacggtg aattcgtcgc cgccgaggcg
    24361 ggcgagtgta tcgccgcggc gcagcgtggc cgacagccgg ctggcgatcg cacgcagcag
    24421 caggtcgccc tcggcgtggc cgaaggtgtc attgaccagc ttgaagcggt cgatgtcgat
    24481 gaacatcacc gcgagcgcgc cggtgcggcg ctgcgcctgc gcgatcgcga gttcgaggcg
    24541 gtccttgaac agcacccggt tgggcaggtg ggtgagctgg tcgtgatagg cctggaagga
    24601 gatgatctcc tcggcgcgct tgcgctcgga gatgtcgcgc gccacgccgt agaggccgat
    24661 cacgcggcgg tcgttctcct gcgtgaccat cgggatgccg gtcagcgaca cggtgacgct
    24721 gccgccctcg ccaccaccgt cgcgccgcga gcgcaggcgc agctcgacgt ggaagctctc
    24781 gcccggcagg ctgctcggct ggctgagcag ggtgtcgatg cggtcgaggt cctcgggcat
    24841 caccagggtg gtgaaggggc gcttgaccag cgcgctgcgg tcgtagccca gcagggcctt
    24901 gatgcgcggg ttgatgtagg tgaagcggcc gctgccgtcg agggtgaaga tcatgtcggg
    24961 cgagctctcc accaggtagc ggtgcaggcg ctccgaatcc ttcaggcgct ggctcatcag
    25021 cttgttggcg cgttcgagcg tgcccttgtg cagggcgccg tcgaccgcgc gctgcagctg
    25081 cgccacgtga tagggcttgc gcacgtagtc gtcggcgccg ccgcgcagcg cgccgatcgc
    25141 cgcgtcgatc gcgtcctcgc cgctgatgat gatcaccgcc tcgtcgcgct ggtggtcgga
    25201 cagccagtcg agcagcgcga ggccgttggc gtcggggaga cggtagtcga gcaggatcag
    25261 cgcgtagcgc tgcttctcca ggcggccgat cgcctcgccg atgctgccgc attcgtcgaa
    25321 gaggcgcccg gggcgggcga gtgcatgcgg aaaggtgcgg cgcagggccg gatcgtcatc
    25381 gacgatcagg atgcggacgc tgtcggcgtc gtcgccggcg gtggcgtcga tccgaggcct
    25441 taacccgttg tcggccggag tgaccatggc tcccggaggc tgcgatgtgg agggcttgtt
    25501 cggccgcatg atgcaacact caggcgcttt gttccagcgg aacgaagatc tggaagctcg
    25561 tgccggcgcc cgcctgggtg cggcacagca gggtgccgcc ccattgtgcg aggatctcgc
    25621 gcaccaccga gaggccgagg ccctggtgct ccgccccctt tgcgctcggg cgcgggctga
    25681 gcggatcctg ggcgcgttcc accggcaggc cggggccgtt gtcgacgaag cggatctcca
    25741 ggcagttgcg gccgtcgaca ttgaccagcg gcagcaccga gacgaccagg cgctggccgg
    25801 gctggagcgc ttcggaggcg ttgcgcagca ggttgagcag cacctgcttg agcgccgatg
    25861 cggggatcgc ggccggcggc acgtcgcgcg cggcgcgcaa ctcgagctgg atctggcggc
    25921 tgccgaagag cggctcgccg tacagcgtgc gcatgtccag cagcagctcg ggcacccggc
    25981 aggtgcgcat ctcggccgtc tcggcaggca gttcggcggc gctgcgcagg agtttgtcga
    26041 tgcggtcgag ttcggcgttg agcaggctca tctcgtcttg cagcgtgctg tcgccggagc
    26101 gctcctgggc gagcatgtcg atgcgggtct tgagcaccgt gagcgggttg ctggcctcgt
    26161 gggcgatgcg gcgcacatgg tcgcggaagc gctggtgcag cgcggcctcg cgctcggcga
    26221 tcgcatcgcg ctggcgggcg tggctgcgca gcgcgcgcgc cgcggcaccg agcagggcgg
    26281 cgtagcgcca gcgcagctcg ctgtcgagcg cctgcgcgga ggcggtggcg acgagggcca
    26341 cgctggtgtc gcccgcgccg cccagaggga tgacgtggaa gcccggctgg ccgagccagc
    26401 gtgcgacctg ccagtcggcg acgctgcgcc cgggcgggcc gccgtcaccg aaatgcgagc
    26461 agggttgctc gctgcgcgcg ccgagggcga tgcagctggt ctcgtcgtcc aggcgcaggc
    26521 gcagctcgcc gatcagcagg tcggggctgc ccgccgcggc gccgagcagc gggcgcaggc
    26581 gaccgtcctc gccgctgccg agcagcaccg gcggggtgtg caggccaaac agcgggcagg
    26641 cgatcgccag gcggtcgcgg atggtcgtcg cgtccaggtc gacgaaggcg gcggtgagca
    26701 ggccgagcat gccggcgtag cggtaggggt cgtccgtgag gcgcagggtc gggcaggccg
    26761 cgcccgtgcc cgcgctgggc aggccgggca gggcggggta ggacgcatgc ccggacagga
    26821 tgtagccgac gtcggtgcgc agcgccacca cgctcgcgct ctcgagtccg gtcagctcgg
    26881 cgacttccgt gatgcgctcc tgccagtcgc caccggcgag ccggcgtgcg gcggcgagca
    26941 ggccgagcag cggatgcatc gcgcccgcgc aggccggatc catgccgtcg aactcgagcg
    27001 cgtcggccag gctcgcgggc aggccgcagt cggcgacaag ggccggcagc gtcggccggc
    27061 gcccggaaac ccaccccgcc gagcgcggca ggccgaacca caggccggcg aggtaggcct
    27121 cgtcggggcg cggatattcg gtctcgatcg cgaggtggag cgcgcattcg gcacgcagca
    27181 gtgcggcgtc ggcggtttcg tccggttggt tgccgacgtg gcccagaccg agcagccagg
    27241 cgcgcagcag gtcggtgccg atgcgctcga ggcggcgccg cagggcggca ttgaggccgt
    27301 cctggagttc gcccgcgcgc agcggatcgg ccagcagcag cgccaggctc agcgcggggt
    27361 cgcgtgcggc cacgagcgcg atctccgccc agttcggcgt ctgcgcggcg agcatgcgca
    27421 ggatcgtcga cgcgccgtcg ggcaggggct ggagctcgaa ggcgctgaaa ttggcggcga
    27481 acatgcgatg gacggttgga aaaagggccg acggattcta gcccgtcggc ccttcccctt
    27541 gccgtgaacg atgtggcggt cgagcgggaa tgacgaacgg ggtgcggcgc tggacgaggt
    27601 tcagccgtgc aggctgtcgg cgaaaccgag cccgaccagc tgtgcgcgat tgacctgctc
    27661 gcagctcagg tggagctggt ccaccgccgc ctgcaaggcc ttggggtcga agctctcgca
    27721 gcactgcgca aggtgcagga agggggcgaa gtcgccttgg ccgtagagga gcgcctcgct
    27781 cacccgcggc ggcaggttca tctcttcgag cagggtctgg atcgaggtgc cgagcagggt
    27841 gtgcagcagc gagaaggcgc cggtgatgaa gaggttgtcg cgctctacgg catcgaagaa
    27901 gccgccgccg atctcttcca tgaagcggcc gcgcgcgatc gcggtctgca tcagcgcggg
    27961 ggcgctgggg tcgcggctgg cggtgaccag cagcagcgac agccatttgt tgagggcgtc
    28021 gtagccgagg atgctgaccg cgtggcggaa ggactggatc tcgaccatga ggccgaagcc
    28081 ggccgagttg atgtagcgca gcagcttgaa ggagagcgcc acgtcctgct tgaggacgcc
    28141 ctcgatctcg cggatgtcgc cattgttgcg caccaggttg agcagccgca cgatctgcgc
    28201 atgccccggc gtgaggctct tggcgggcgg gttgccgtgc aggaagaacc agcccgaggc
    28261 accgtcgtag cccgccgcga cggcctggcg gaaggcgccg atgtcggcca ggccccaggc
    28321 caggctcagg ccgggcgagc cggtgggcgc agggtggcgg cgcgcgtcga tgagcacgaa
    28381 acgccagtcg tggccggcgg gcagggcgac ctcgggctgg aaccaggtca ggcacatgcc
    28441 gatgccggca tcgcgcaggc gcgccatcag cgcctgggtg tgcggatggg cgagcgtctg
    28501 caccgggatc tcgatcgcca cgttcccggg gatcgcccac tccatgatct cgggcgtcgg
    28561 caccaggcgg ccgaggctga cgaacaccgg atggtgggtc ggccacaccg cggcctgggc
    28621 ggagagcgcc tcgaccaccg aggcgatgtt ggggccgtgg gcgatcaggc ggttggcggt
    28681 gatcgcgcgg ttcttgttga cgaccggctc gcgggtcagc agggtggtct ggctcatcgg
    28741 gcgttccggg tcatcgggcg gagggctggg cgaaggtgaa ggcgtcggcg aagaacgcgt
    28801 cctcgggcag gccgcgttcg ctgtggaagc tggcgcgtgc ggcatcgacc atcgcggggg
    28861 cgccgcagac atagacctcg tgcgcggaaa ggtcggcgaa gtcttcgagt accgcctggt
    28921 gcaccaggcc gccgcgtccc gcccaggcct cgtcggagag caccgggacg tagcgcaggc
    28981 cgggcagcac ctcctcccag gcgcgtgcga gcgcgtcgag atacagcccg tcgcgccggc
    29041 gcgcacccca atacagcgtg atcgggcgct gcaggccgag ccggatcgcg tgctcgacga
    29101 tgcccttgat cggtgcgaag ccggtgccgc cggccaccag cacgatcggc cgcgcggagt
    29161 cctcgcgcag gaagaagctg cccagcggcc cttcgaagcg caggatctcc ttttccatca
    29221 tggtctcgaa gacgtggccg gtgaaacggc cgccgtcgat gcggcgcaca tgcagctcga
    29281 gatggcgggc gtcgtgcggg gcgttggcga tcgagaagct gcggcgctgg ccgtcggcga
    29341 gcaggatgtc gatgtactgg ccggcgcgga aggcgaagtt ctcgctcgcg ggcagcttga
    29401 gctcgatccg catcacgtcc tcggccagac gctcgagctt ctgcacgcgg cagggcagct
    29461 tcttcacctg gatgtcgccg gcgcgcgtca cgctgcgtgc ctgcaccatc acgtcgccga
    29521 gcggcttcgc gcagcagaac agcgccagac cctcggcgcg ctcggcctcg ctcagtgcgc
    29581 cgggcgagtg ctcgccgtgc tcgacctcgc cctcgagcac gcgacccttg caggcgccgc
    29641 aggcgccgtc gcggcagccg tgcggcagca gcaggccggc ggcgagcgcg gcctcgagca
    29701 cggtctggtc gtcgtcggcc tcgaaggagt ggttgccggg ctggagggaa atccggtggc
    29761 tcatggtgcg tgagataatc gaacgatgaa aaggatactg atcgtcggca gcggcgacgt
    29821 cgcccgccgc gcgctgccat ggctggtgcg tcgctttcgt gtcttcgcgc tcgtccgcca
    29881 gcccgaggcg gccgccgaac tgcgtgcgct cggcgcggtg ccggtcgcgg gcgacctcga
    29941 cgaccgccgc agcctgcgcc gcctggccgg catcgccgac gcggtgctgc acttcgcgcc
    30001 gccgccgcag acgggcgagg gcgacccgcg catggcgcgg ctgctggccg cgctggcagg
    30061 tcgatcgagt ctaccacagg gggtggtata cataagcacg acaggggttt acggagactg
    30121 cgcaggtgtc cgggtggatg agacgcggcc gtgccgggcg cagaccgcgc gcggccgccg
    30181 acgggtggat gccgagcgcc gcctgcgcgc cttcggccgc cgtaaccggg tgcgggtcgc
    30241 cctgctgcgc gcgcccggca tctacgccgc cgaccgtctg ccgctcgagc gcctgcgtcg
    30301 cggcgatccg gtgctcgccg ccgacgagga tgtgcacacc aaccacatcc acgccgagga
    30361 cctcgcccgc atcgcctgcc tggcgctgtt ccgtgccggg ccggggcggg cctacaacgc
    30421 cagcgacgac tccggcctgc gcatgggcga gtacttcgac gcggtcgccg agcgtttcgg
    30481 cctggcgcgc ccgccacgca tggcacgcgc cgagatcgtc cgccacctgt cgccgctcgc
    30541 gctgtccttc atgagcgagt cgcgccgcct cgacaaccgc cggctgaagc gggaactgcg
    30601 cctgcacctg cgctatccga cggtggccga cggcttgcgc gccgcgaccg ttcccgtctg
    30661 aaccgcggcg ctttgccgcg ccgcgggtga acttccccga tcgaacctgg agcccttccc
    30721 gcatgctgac cctcaaggcc tttcacatcg tcttcgtcgt gagctggttc gcaggcctgt
    30781 tctacctgcc acgcctcttc gtcaatcacg ccatggtcga ggacgccgcc acccgcgagc
    30841 gcctggcgat gatggagatg aagctctacc gcttcatgac gccgctcggc atcctcgccg
    30901 tgggcctcgg cctgtggctg tggttcggct acggcttcgc cggcggctgg ctgcacgtga
    30961 agaccctgct ggtggtgttc ctgatcgcct accacctgta ttgcggccgc ctgatgcgcg
    31021 ccttcgccga gggacgcaac acgcgcagcc acgtgtggta ccgctatttc aacgagatcc
    31081 cggtgctggt gctgttcgtg gtggtgttcc tcgtggtctt gaagcccttc tgaacatggt
    31141 cgcgaaacgg atcgggggcg gcggggacga cgtgcagatg gcgcacggcg tgcggggcga
    31201 cgaggcgaag gcgctcgcgc aaggtggagt cgggcgcgcc gagcccgcgc gcccggtgga
    31261 gctcgccgac taccagtggc ctgcgcactc ggtgctggtg gtcgacgacg aggagggcat
    31321 gcgcaatttc ctcgagcgca cgctcgcgcg ccgctgcggc atggtgcagt cggccgccga
    31381 tgccgagcac gccgcggtgc tgatggcgcg tctgcatttc gacctgctgg tcctcgacat
    31441 cgccctgcct ggcaagtcgg ggatcgagtg gctgcacgag ctgcgcgaga atggttatgc
    31501 cggcgacgtg atcctgatca ccgccttcgc cgacatggag accgcgatcg acgcgctgcg
    31561 cggcggcgcc tcggacttca tcctcaagcc cttccgcgtc gaccagatcc tcaactcgat
    31621 caagcgctgc ttcgagcgtg ccggcctggc gcgcgagaac ttcgtgctgc gccgcgagct
    31681 cgccggcctg ggcgccgagc ccaccgggct gatcggccac tcgcccgcga tggagcagct
    31741 gcgcgcgctg gtgcgccgcg tcgggcagat gccgagcacg gtgctgctgc tgggcgaatc
    31801 cggtaccggc aaggaggtgg tggcgcgcgc gttgcaccag accagcccgc gcgcgcagcg
    31861 ccccttcgtg ccgctcaact gcgcggcgat cgcctccgag ctgatcgaga gcgagctctt
    31921 cggccacgtc aagggcgcct tcaccggcgc caccgagaac cgcaacggcc tgttctacta
    31981 cgcccacggc ggcacgctct tcctcgacga gatcagcgag ctgccgctgg cgatgcagac
    32041 ccggctgctg cgggtactcg aggagcgcaa gctgcgcccg gtcggttccg agcgcgagct
    32101 gccggtggat gtacgcatca tcgccgcctc caaccgcgac ctcgccgccg aggtgcgcgc
    32161 cggacgcttc cgcgaggacc tctactaccg cctcgcggtg gtggacatcg gcctgccgcc
    32221 gctgcgcgac cgtaccgagg acatccccga gctgatgcgc cacttcatgc agcagttttc
    32281 cgtccagctc ggcgtgccgc cgctgccgct ctcgcacgag gtggtgagcc ggctcgccgg
    32341 ctacacctgg cctggcaacg tgcgcgaact gcgcaactac atcgagcgct cgctgattct
    32401 cggccacttc ccggcgcagc cgagcgccgc accgaccgcc gcgccgacgc cggcgggcga
    32461 gctggaggcg agcctggccg aggtcgagcg ccgccacatc gagcgtgtga ccgccgcctg
    32521 cgagggcaac aagaccgagg ccgcgcgccg gctgggcgtg tcgcgcaaga ccctggagcg
    32581 caagttcgcc gagtgggcgc tcgaggacgc cgcggccgcg cgtgcggagc ggcgggcctg
    32641 agcgtggcgg gggtcggggc gagcaggctg cgccggatgg cggggcccgg tgcgggagcg
    32701 gggcgatgag gctcgccgaa cgcctgcgcg gctcggtacg cgccaagctg ctcgccctcg
    32761 tgctcgcgcc gctgatgctg ggcttcccgc tgatcatggg cgtgctgtgg tactggggcg
    32821 aggtctacta ccaccgcctg atggtgtcgc gggtcgccag cgacctggcg accgcgcacg
    32881 gttacttcga gcgcgtgatc gacggcgtcg gcaacggcgt gcaggggctc gccggctcgc
    32941 atgcgctcgc gcagaccctg gcgcacgaga gtccggaagc ggtggccgcg ctgctcggtg
    33001 cgcgcaaggg cggactcgcg ctcgacttcc tcaacctgct cgacgtcaat ggccgcgtgc
    33061 tgcatgcgtc gaccgcgctg gcggcgggga gcgcgcgtgg tggatggccc gtcgtgcagc
    33121 aggcgatccg cgcccgcggc ggcagcgtcg tcgagcgctt cgcacccgcc gagctcgcgg
    33181 cgatcgacgc cggcatgcgt gagcgtgccc gccttgccct ggtggccacg gcgcgcgcgc
    33241 ggccggattc gcgcggcgag gaggagcgcg gcctggtggt gcacgcggcg gccccggtgc
    33301 tcgacgccgc gggcaacctg gtcgcggtgc tcgaaggcgg cgtgctgctc aatggcaacc
    33361 tcgacttcgt cgataccctc aacgccatcg tctaccgcga gggcagcctg cccgagggca
    33421 gcgagggcac cgccacgctc ttcgtcgacg atgtgcgcat cgccaccaac gtgcgcctgt
    33481 tcgagggcgc gcgcgcgctc ggcacgcgcg cctcggacga ggtccgcacg catgtgctcg
    33541 accacgggcg tacctggctg gagaccgcct tcgtggtcaa cgactggtac gtgtcggctt
    33601 acgagccggt ggtggacagc cgtggcgaac gcgtcggcat gctctacgtc ggcttcctcg
    33661 aggcgccctt ccgcgccgcc aagcgcatgg ccctgctggt ggtcggcgcg ctcgcgctgc
    33721 tggtcagcgc cgccggcgcg ctgctgacgc tgtattgggc gcgcggcatc ttccgcccga
    33781 tcgagcgcat gcacgccacc atcggccgca tcgacgccgg cgacgagcag gcgcgcgtcg
    33841 gcgacgtcgc cagccgcgac gagctcggcc gcctggctac cgccttcgac cacctgctcg
    33901 acgaccaggc cgcgcgccgc gccgagctgc aggcgctcaa cgcctcgctc gaccgcaagg
    33961 tcgccgagcg caccgccgac ctcgccgacg ccaacgccga gctgcgcgcc gcacagcacc
    34021 gcctggtgat gagcgagaag ctcgccgcga tcggcgagct caccgccggc gtcgcgcacg
    34081 agatcagcaa tcccaccgcg gtgatccagg gcaatctcga cctgctgcgc gaggaactcg
    34141 gccccgcggc gcagccggtg gccaacgaga tccgcctgat ccacgagcag gtcgggcgca
    34201 tccgcctgat cgtcaccaag ctgctgcagt tcgcccgtcc gggcgagttc gccggctacg
    34261 tcgaggacgt cgacgtgaac gccgcgctcg ccgactgcct ggtgctgacg cgccagcatc
    34321 tggcgcgcgc cgaggtgaag gtggtgcagc gcctggccgc caccgcgcgg gtgcagatca
    34381 acctgcagga gctgcagcag gtgctgatca acctcatcgt caacgccgtg caggccatgc
    34441 cggccggcgg tacgctgacg ctggagacgg tcgaccgcga cccgcggcag gatgcgcgcg
    34501 gcgtgcgcat cacggtgcgc gacaccggcg gcggcatccg tgccgaggac ctggcgcgca
    34561 tcttcgatcc cttctttacc accaagaagc gccaaggcac cgggctgggg ctgtcgatca
    34621 gccacaccct ggtcgagcgc tacggcggcc gcatcgaggt ggacagcgcg cccgggcggg
    34681 gcgcggcctt cacggtgagc ctgctcgccg agccggtgta tcgcaaggac cccgcgccgc
    34741 cgcccggcgg acgggagaac ggaacaggac aaacggaagc gacatgaaag tgatgggttt
    34801 cgccggctgg tccggcagcg gcaagacgac gctggtggag caggtgatcg cggcgctgag
    34861 cgcgcgcggc ctggtggtgt cgctggtcaa gcacgcccac cacagcttcg acatcgacca
    34921 cgccggcaag gactcctggc gccatcgcca ggccggctgc cgcgaggtca tggtgagctc
    34981 ggaccagcgc tggtcgctga cacgcgagct gcgcggcggg cccgaggcga cgctcgacga
    35041 gctgcttgcc cacctcggac cgtgcgatct ggtgttggtc gagggcttca agcgcgcggc
    35101 gatccccaag atcgaggtgc atcgccgggg gctcgccgag ccgctgctgt atccgcacga
    35161 tccgcacatc gtcgccatcg cgaccgacgc cgagttgccc gcaccgctgc cgcggctgga
    35221 cctcaacgac ccggtgcagg tggccgactt catcgcggac catctgcacc tgtagtgcgg
    35281 gcgaggtcgg tccagtctgt tgcatccatg cgaacgcccg gtatggcagg gcgaatgctc
    35341 tgttaggatt ctcgagataa ataattttct cgagagacgg taatgaggat cgaaaagacc
    35401 acggcgggga cgccccaccg catcgtcatc gtcggcgggg gcgccggcgg actggagctg
    35461 gcgacacgcc tggggcgcaa gttcggccgc ggcgggcggg tcgaggtcac cctcgtcgac
    35521 agcaagcgca cccacgtgtg gaagcccaag ctccacgaga tcgctgccgg cagcatggac
    35581 atcggcgatc acgaggtgcc ctacctggcg caggcgcact ggcatggctt ccgcttccgc
    35641 atcgggcaga tgatcgggct cgaccgcagc cggcgcgaga tccacgtcgc ccccttcgtc
    35701 gatgaagacg gcaaggaggt cacgcccgcg cgttccttcc cctacgacac gctggtgatc
    35761 gcggtcggca gtctcagcaa cgatttcggc acgccggggg tgcgcgagca tgcgctcaag
    35821 ctcgagaccg tcgccgatgc gcggcgtttc catgtgcgca tggtcaatgc gtgcgtgcgc
    35881 gcccacgccc agcgttcgcc gctgcgcccc gggcagttgc atgtggcgat catcggtgcc
    35941 ggcgctaccg gcgtcgagct tgccgccgag ttgcaccaca ccacgcgcga ggtggtggcg
    36001 tacgggctgg accgcgtcga tgccgaaaag gacgtccggc tcaccctgat cgaagccgcg
    36061 ccgcgcgtgc tgccggcctt gcccgagcgc ctgtcgcgat cgaccgagcg cttgctgcgc
    36121 aagctgggtg tggaagtgaa taccgacgca cgcgtggccg aggtgctgcc cgatggcgtg
    36181 cgcctggccg acggtcgcgt cctgcccgcc gagctggtgg tgtgggcggc cggggtgaag
    36241 gcgcccgatt tcctcacccg gctcgacggg ctggagacca atcgcatcaa ccagctcgtg
    36301 gtgcggcaga ccctgcagag ttcggtagac gacgacatct tcgcgatggg cgactgcgcc
    36361 gcctgcccgt ggccggaaaa ggacggcttc gtgcccccgc gcgcgcaggc cgcgcaccag
    36421 caggcctcgc atctgtacac gcagctgcag cgccgcctcg ccggcaagcc ggtgcaggac
    36481 taccgctacc gggacttcgg ttcgctggtg tcgctgggcg agttcagcac ggtgggcaac
    36541 atgatgggcg ggctcgccag gggcgacctg ttcatcgagg gctggttcgc gcgcctgatg
    36601 tatgcctcct tgtacaagat gcacgaactg gcgctgcacg gctgggtgaa ggtgacgctc
    36661 gacaccttgg cgcgcatgat cacgcgccgc accgagcccc acgtcaagct gcactgaggc
    36721 cgcgcgtgcc gcgccctcag tcgaggccga gcacgcgccc gagcgggccg aggtcgtagc
    36781 cgccgagccg gtgcgccagc gcctgagagc gcgcgtcgcc gtgcgcggcg agcagatcct
    36841 ggaagaggcg gcggaacatc acgtcgcgcg gcagcgcgag gtcgaaggcc tcccagccca
    36901 agggcaggaa gccgaggccg aactcggtcg ctgcggcgcg cgtgccgggc gcgcagtcgg
    36961 cgtcctcgcg tgcgaccagg gccgcggcct cgcgctcgct gcgggcggtg gcgaccacgc
    37021 tgcagtcggc cgccgagaaa ccgcggctgg cgagcacgga ctcgagaaag tgcgccgaac
    37081 ccgcgccggc ctggcgcatc gcccagcgat agtcgaaggc ggcgagcgtc tcgatgccgt
    37141 cgaccgccag gccgcggcgc aggatcacgc cctgctcgcg cagcgccagg cgcaccagcg
    37201 cccaatggcg atgcggggcg aagcggcgca gcagcatggc gtgctgctgc aggctgtgct
    37261 ccaccccgcc ccagtgcagc gcacacacgc tggcgcggcg gcgcgcgagc agctcgagcc
    37321 cgggcagggt gccggtgggg ctgtaggcga cgaaggcatc gccccccagc acgggagcga
    37381 gcgcgcccac cgtggcggcc agcagtgggt cgtcgctgcc ggtgatcaac agccggtcgc
    37441 tgagtgcgcc gccatgcgcc tgctcgagca gccactcctc gagcagtgcg cgcgggaaca
    37501 gccacttgcc gcccacgcgg gcggcgggaa tctcgcggct gttggcgagt tcgtagagct
    37561 tcttctcgtt gagctgcagg aaggccgcgg cctcgcgtgc gttgaggcag gtcgcggatg
    37621 ccgcgtcggt gcctgccggc tgcgcggggc gcgcgctcat ccgcgcggcg cgcccgcggc
    37681 ggcggcgggc aggtcgcggg cggcctcggc cagctcgtcg cgggtgttga cgttgcggaa
    37741 ggcggcctcc tcgtcgtcga aggcgacctc gaccaccttc aggctgccgt accagcggtc
    37801 gatcttgcgt ccgccggcgg cgaggaaggc accgaggtga tcggcgagat tgcgccggca
    37861 caggcagaac accgggtgcg gctgcccgaa ggtcttggcg acggcgagct gggcgtcggc
    37921 ggcgtggagt gcggcggcca ggcgctcgac cagatccgcg ggcaggaacg gggagtcgca
    37981 gggcgcggtg acgacgaagg gatgctgcgc gcgcaggagc ccggcatgga gcccggcgag
    38041 cgggccggcg aagccgccga tgtcgtcgcc gaacaccgga tggccgaagg cctgccaggc
    38101 cggaaggttc tggttggcgt tgatgagcag cgcgtcgacc tggggggcga ggcgctcgag
    38161 cgcgtgtgcc gccatcggcc gcccggcgag ctcgaccagg cccttgtcca caccgcccat
    38221 gcggctgccc tggccgccag cgagcaggat cccggtgatg cggcggtcgg cggggcgggg
    38281 ggctggggag ggaggcatgg tgggagctcg tcgtgcgtgg gagggggtgg cgcttcagtc
    38341 gcgctcgaag cggtcggcgc cggagaacag caggtagtgc ttgccctggg cgcggccgat
    38401 catggtgagg ccgatcttct gcgcgatctg ccagcccatg tgggtgaggc cggagcgcga
    38461 gacgaggaag ggaatgccca tctgcgcggt cttgatcacc atctcggagg tgagccggcc
    38521 ggtggtgtag aagatcttgt cgctgccatc gagcccgtcg agccacatct ggccggcgat
    38581 cgcgtccacc gcgttgtggc ggccgacgtc ctcgaagaac atcaggatct cgcagccgcc
    38641 gggcgtggcc ctggcgagcg cgcagccgtg caccgcgccg gcctgcttgt agatggactc
    38701 gtggtggcgc acggcgtcca tcagggcgta gagggtggat tgccgcagct ggcgatcgtg
    38761 cggcaggcgg atctggtcga tctcgtccat gagtccgccg aacaccgtgc cctggccgca
    38821 gccggtcgtg accgtgcggc tgccgagctt gcgctcggcg tcctgcatgc cggcgcgcgt
    38881 ggtgatcgcg accgcgtcgg tatcccagtc cacctgcacc gcggcgatct cgtccaggct
    38941 gcgcaccagg cgctggttgc gcagccagcc gatggcgagc gcctcggggg cggcgccgag
    39001 cgtcatcagg gtgacgatct cgcgcttgtc gacgtagagc gtgagcggat gctcgccggc
    39061 gatcgaggtc ggcaccgtgt cgccgcgttc atcgaccgcg gggatctcga tggtgagtgg
    39121 acggcgggcc tggctcagca ggggacggtg gggaggggtc atcggggcgg ggccggggat
    39181 gcgcgagcag gcgattgtag ccgcaagtcc ggcgaggcat gggcggagtc gcggctccaa
    39241 tccggccgtg cggtcgctgc ggtgatccgc cctcgggacc acggcggcgc gcgccgcctg
    39301 cactcgcggc ctcgccccga cacggaattc gctaccgcgg atcgcgactc acgtggcgat
    39361 tcccgcacgc gcggcggtcg gacatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39481 nnnnncggat gcggcggggg gcgccgaggc gcaattgccg ccggtcgaga gcttgtcgct
    39541 gtcctcggat ttcaccgcct tcctgaagga agaggtgagc gaggcgctgc gtcgcaaggc
    39601 cttgcagaaa ctgttctccg acccgcactt caatcgcatg gacgggctgg acatctacat
    39661 cgacgactac tcgcagcccg acccgatccc gccggagatc ctggcgaagc tccagcatgc
    39721 gcgcgaatgg ctgatggacg acgcgacggc actgcccgct cccgaggggg cgacggcgcc
    39781 cgtcgacggc gagcggggac gggggaccac cgcggaaccg gccactgcgg tggagtgtgc
    39841 gtctgcgcaa gctgcgactg ctccgggtgg gcgatgcgtc gacgatctgt cgggcgcggt
    39901 gtcgtcggag ccgccgcaag agtcgggaca aaacgcaccc tgagcgacaa attgtcgcgc
    39961 gtcgattccg gatatcgccc agtcatggct tttcggggat tcctgcggtc ttctctgagt
    40021 ccccggctgg cctatgaact gctttgcgta agggaatcga tggaagaaca catgagcgaa
    40081 cgccgcgacc cgacctcccg tatccacctg tgcgattgca accgcagctt cgcgctcgac
    40141 gccgggcgtc ttgcggcggg cacggatgcc gcgatgcagt gtcatcacgc gctctgcggc
    40201 gccgaactcc cggcgctgca aacttcgctc gcaggcggaa agaccgtgca tgtcgcctgt
    40261 acgcaggaaa ccgcgctctt cggcgaactc gcggaggaag ccggcgcggg cgagcgcatc
    40321 cgcttcttca acctgcgcga gcacgccggc tggtcggccg agtccgcggc ggcaacgccc
    40381 aagctcgccg cgctgatcgc ggcggccacc accttggccg accccgagcc ggtggctgcg
    40441 gtgcagatga gcgccgggcg cagcctgctg atcatcggcg aggcgggcgc ggcgctgggc
    40501 tgggcggagc gcctggccgg cagcttcgag ccggcggtgc tgatcaccgc gcgctcgaag
    40561 gacgtggagc tgccggcggt caacgcgtac ccggtgtggt cgggcaggcc ggtgcggctc
    40621 ggcggccacc tcggcgcctt cgagctggag tggacgcagc acaacccgat cgatctcgag
    40681 ctgtgcgtgc gctgcaacgc ctgcgtcaag gcctgtcccg agggcgcgat cggctgggac
    40741 ctgcaggtcg atgtcgacgc ctgtcgcagc cacggcgcct gcgtcgccgc ctgtggcgag
    40801 atcggcgcga tcgacttcgc gcgccaggac agcgcgcgca gcgaacgctt cgacctcgtg
    40861 ctcgacctgt cggccgagcc gctgctgcgt cgcgtcgagc tgcccgacgg ctacgccgcg
    40921 cctgggcgcg atcccttcga gcaggcgctc gcggtgcagg cgctggggga gttcgtcggc
    40981 gagttcgaga agccgcgcta cgtagccttc gaggccgccc tgtgcgcgca cagccgggcg
    41041 aagaagaccg gctgcagcaa ctgcatcgag tcctgttcca ccgcggcgat ccgctcgaac
    41101 ggcgacgtga tcgcggtcga cccgtatctg tgcaagggct gcggcacctg cagcacggtg
    41161 tgtccctcgg gcgcgctgtc cttccagtat ccgcgcgtgc ccgagctcgg ccagcgcgtg
    41221 aagaccctgc tcgccgaata cgcgcgcgcc ggcggcaccg acgcctgcct gctgtttcat
    41281 tccgccgcgg ccggcagcgc cgcgatcgcc cgcctcgcgc gccgcggccg cggcctgccg
    41341 gcgcgggtga tcccggtcga ggtgtggagc gccgacgcgg tcgggctgga cctgatgctc
    41401 ggcagcctcg cgctcggcgc ctgtcaggtg gcggtgctcg ccgccggcag ccacgacgcc
    41461 gcgccgctcg aggcccaggc gggtcatggc caggccatcc tctccgccct gggttacggc
    41521 ggcgaacacc tgcgcgtgat cgaggccgac gccgacgact ggcaggcgct cgagcgcgca
    41581 ctgtacgact gggctccggc gatgaccgtc gccgaacccg cgcgtttccg gttgctggcg
    41641 aagaagcgcg agacgctgga cttcgcgctc cgccatctgg tcgcccacgc gcctgccacg
    41701 accacgcctg cgggcctgcc ggcggcgatc gcgctcaacg cgggcgcacc cttcggccag
    41761 gtgatcgcat ccgacgcctg taccttgtgc atgtcctgta ccggcgcctg tccggcgggc
    41821 gcgctgcgcg ccgccagcga cgcctaccgc ctcgagttcg tcgagaagaa ctgcctgcag
    41881 tgcggcctgt gcgaggcgtc ctgcccggag tccgcgatca cgctcgagcc gcgcctgctg
    41941 ccgggcgagt acggcaccga aggtgcgcgc cgcgcgcgcg ccctgcgcga agccgacatc
    42001 ttccactgca ccgcctgcgg caaggccatg ggcgccgcgc cgctgatcga atccatgatc
    42061 gcccgcctgt ccggccacgc catgttcgcc ggcgaggccg agcgcgcgcg cctgcgcatg
    42121 tgcgccgact gccgggtgat cgacatgatg aaggccgaaa cggccgtcaa ggcctgggac
    42181 ctcactgaat gaacgactcc gaatgagcgc agccgcgaac gccagcattt ccatccagcc
    42241 ccagccgacc tggtcggacg aggaccgcgc gcgcgccgag cactacgcgc tgatcgcccg
    42301 tctgttccac gccgcgcccg acgctgcgct gctcgcggcg ctcgcgcagg cggggcggac
    42361 gctcggcggc gagggcggcg agctgccgcg tgcctgggcc gctttgggcg cggcggcgca
    42421 gacgatgccg ctgcaggcgg tggccgagga gttcgacgcc ttgttcgtca gcgtcggcaa
    42481 gcccgaggtg atgtgcaacg gctcctggta cctgaccggc ttcctgcagg aagagccgct
    42541 cgccgagctg cgcgacgacc tcgccgaact cggcctgggc cgccgcgcgg gcgtgaccga
    42601 gaccgaggac cacatcgccg cactcgccga ggtcatgcgc cacctggtgc tgaccgggcc
    42661 ggacgacgcg gggctggcgc gccagcggcg cttcttcggc cgccacctcg cgccctggta
    42721 cgcgcagttc gccggcgcgc ttgcggcggc gccgggggcg gatttctacg cgaaggtcgg
    42781 cggcctgctg gcggccttca tggacatcga gcgcgaggcg ttcgacatgc tggaggcgga
    42841 agcgcgctga gcgcggccgc catggacacg atgcggggcg ggcgggcgcc tgcgccgcga
    42901 atgaggggag agcgaggatg aaggacgaaa acaccaagct gtcgcgccgc aacttcctgg
    42961 ccgcgatcgg tgccggcagt gccgctggcg ccgccacgct ggccgcgacc gccgcccagg
    43021 cgtcgcctgt caccgccaag gtggtcgagg aggcgcgccg cgaggtcgcc gaccagggcg
    43081 tgagcgagca catgcgcaat tactaccgca cgacgcggat ctgaggagga cgcgaggatg
    43141 ttgacgaaga aatccgcgac cgccgccacc agcgcgcgtc gcctgcgcgg cgccgccgcg
    43201 cgccagctcg gccagaccat ggaccgccgc gccttcctga agcgctccgg cctgggcgtg
    43261 ggcgccggcg cgctcgccac ccagctgccc tacaacttca tcggcgccgc cgacgccgcg
    43321 gcgcccgcgg gcgtgtcgcg cgccgagggc aaggccgaga tccgccgcac cgtgtgcacc
    43381 cactgctcgg tgggctgtgc cgccgacgcg gtggtgcgca acggcgtgtg ggtgcatcag
    43441 gagccggtgt tcgactcgcc gatcaacctc gggtcgcact gcgccaaggg cgccgcgctg
    43501 cgcgagcatg gccacggtga ataccgcctg aagtatccga tgaagctggt cgacggcaag
    43561 taccagaaga tctcgtggga gcaggcgctc aacgaggtcg gcgaccgcct gctgaagatc
    43621 cgcgaggagt ccggccccga cgcggtgtat ttcatcggat cgtcgaaaca taacaacgag
    43681 caggcctacc tgctgcgcaa gttcgtctcc ttctggggca ccaacaacac cgaccaccag
    43741 gcgcgcatct gccactccac caccgtggcg ggcgtggcga acacctgggg ctacggcgcg
    43801 atgacgaact cctacaacga tatgcagaac gccaaggcga tgctgttcat cggctcgaac
    43861 gcggccgagg cgcacccggt gtccttgttg cacatcctgc acgccaagga aaacggcgcc
    43921 aagatgatcg tcgttgaccc gcgcttcacc cgcaccgcgg ccaaggccca ccagtacatc
    43981 cgcatccgct ccggcaccga cgttcccttc ctgttcggcc tgctgtacca catcttccag
    44041 aacggctggg aagacaagca gtacatcgac gaccgcgtgt tcggcatgga caagatccgc
    44101 gaggaagtgc tcgccaagtg gacgccggac aaggtggaag aagcctgcgg tgtgcccgcg
    44161 gagcagatgc tgctcgccgc ccgcaccatg gccgagaacc gcccgagcac catcgtctgg
    44221 tgcatgggcc agacccagca ctcgatcggc aacgcgatgg tgcgcgcctc ctgcatcctg
    44281 cagctcgcgc tcggcaacat cggcaagtcg ggcggcggcg ccaacatctt ccgcggccac
    44341 gacaacgtgc agggcgcgac cgacgtcggc cccaaccccg attccctgcc cggctactac
    44401 ggcctcgcca ccggctcgtg gaagcactgg gcgaacgtgt ggggcgtgga ctacgagtgg
    44461 gtcaagggtc gctacgtctc gcaggagctg atggagaagt ccggcatcac cgtgtcgcgc
    44521 tggatcgacg gcgtgctcga gaacaaggac ctcatcgacc agcccgccgg caacctgcgc
    44581 gcggtggtgt attggggtca tgcgcccaac tcgcagacgc gcggcgccga gatggtcgag
    44641 gcgatgaaga agctcgacac cctggtggtg atcgacccct atccgtcggc caccgcggcg
    44701 atggcggcga tggtgcggaa ggacggcgtg tacctgctgc cggcctgcac ccagttcgag
    44761 acccacggct cggcgaccgc gtcgaaccgc tcgatccagt ggcgcgagaa ggtcatcgag
    44821 cccctcttcg agtccaagcc cgatcacacc atcatgtacg ccttcgcgaa gaagttcggc
    44881 tgggccgagc agttcgtgaa gaacatcaag ctcgagaagg atgcccaggg ctgggacgag
    44941 ccgagcatcg aggacacgct gcgcgagatc aaccgcggca cctggacgat cggctacacc
    45001 ggccagtcgc ccgagcgcct caagctgcac atgaagaac
//
LOCUS       DAOQBS010000010        44177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_417_length_44177_cov_14.813517, whole genome shotgun sequence.
ACCESSION   DAOQBS010000010 DAOQBS010000000
VERSION     DAOQBS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 44177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44177
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_417_length_44177_cov_14.813517"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..494
                     /locus_tag="PKN25_02345"
     CDS             174..494
                     /locus_tag="PKN25_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNV89766.1"
                     /translation="MKTRFRSLLAAAALLVAGGLSVSGASAGERVRVEEIRQLRESGQ
                     ILSAEDILARSRAIQPGQVVGLELEREDGRWIYEVKLIDERNRVHELELDAANGEVLE
                     RKEK"
     gene            498..1163
                     /locus_tag="PKN25_02350"
     CDS             498..1163
                     /locus_tag="PKN25_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV89767.1"
                     /translation="MRLLVVEDDAALGERLRTRLVAEGYAVDLARDGIDGAHLGATEP
                     YDAVILDLGLPGKPGLTVLREWRAAANRVPVLILTARDGWAERVEGLQAGADDYLGKP
                     FHVEELLARIQALLRRAAPVPDTELRAGDWRLDEARQCLVDRAGNEQALTATEFRLLR
                     YFMRHPDEVLSKTRLSEHVYDYDAERDSNVIEVYVRRLRGLLGNERIETRRGQGYVFR
                     SGD"
     gene            1191..2546
                     /locus_tag="PKN25_02355"
     CDS             1191..2546
                     /locus_tag="PKN25_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV89768.1"
                     /translation="MNSIRRRLSLTLALVLLAAGVLLAFALRDFPRRMVEEHVLARLD
                     HDADLLYVHLLDALARQDGEAGMDSALMGAAGPAYDLPLSGHYFLVRLGDRQWRSRST
                     WDADLDLPEGAEGLVSPRLAGPAGQALLVVAKRFPDSADGRAVSIALAEDVAAIDAAI
                     AAFRAQALTVLGLALLVLLVLQRRVLLRGLAPLDEAVDACRRLERGEAVQFEADAPAE
                     VRPLLAAVERLSRHQAQRLGRIRHAAGNLSHALKTPLAVLGHSADALAARGDGEGAAA
                     IRTQLETMRATIERELRRARLAGGAAGGAGFALRAQLGALVEVLQRLHAGRGLRIELE
                     ADERTWPVDREDMLELFGNLLDNACKWARARVRVVVAPDAGTARLAFSVEDDGPGVAP
                     ELLERLGTAGLRADESRPGHGLGLAIVGDIVTQYGGTLRFGRGSVLGGLRVDVELPLA
                     V"
     gene            2670..>3253
                     /locus_tag="PKN25_02360"
     CDS             2670..>3253
                     /locus_tag="PKN25_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNV89769.1"
                     /translation="MRAKPLPAETRRAVTVEAVIELAAERDPGEITTAAIASHMKLTQ
                     GALFRHFPSKDAIWEAVMEWVAERLLARVDRAAAQAASPLAALQAIFLAHADFVAEHP
                     GVPRMLFGELQRAEATPAKRLARALLERYGERIRGRLEAGKAAGEVSAALDIDAAVIL
                     FVGTLQGLIMQSMLAGDLLRIRRDAPRVFAIYLAG"
     assembly_gap    3254..3353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3354..4245
                     /locus_tag="PKN25_02365"
     CDS             <3354..4245
                     /locus_tag="PKN25_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV89770.1"
                     /translation="GQVLGEMDAVDLGDRIRAQEAALERAQAAMREAEARQAFARAQA
                     RRYDKLGSLQAASEELALTRRQELEVAEAGLAAAREERTRARAEGAALQAQLANLRLV
                     APVDGVVTLREVEPGTTVVAGQAVLEVIDPATLWVDLRLDQVNAGGLAAGLPARVVLR
                     SRSGEALAGRVLRVEPKADAVTEELLAKVVFDRLPEPMPPLGELAEVTLALPPLHAAA
                     VIPNAALRREAGAVGVWRRAGEGGLRFVPLKLGASDLDGRVQVREGLAAGDEIVVYSE
                     AGLRAGRRTEVVAALDGAGK"
     gene            4242..5447
                     /locus_tag="PKN25_02370"
     CDS             4242..5447
                     /locus_tag="PKN25_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV89771.1"
                     /translation="MISLAGRDILHGWGKFVFTGVGLGLLIGVTLVMAGVYRGMVDDG
                     KALLDNSGADLWVVQRDTLGPYAESSSVHDDLYRSLLGQRGVAGAANITYLTMQVRKG
                     SEDVRAMVVGIAAGRPGATPGWPPYLVAGRQITRGHYEAVADVASGLRLGERVGIRRN
                     QYTVVGLTRRMVSSAGDPMVFIPLKDAQEAQFLKDNDAILQGRRRTAANPALNRPGVP
                     GLLDAVLASQSTNPYVNAVLLTLAPGHAPDEVAESIRRWQRLTVYTRAQMEGILVGKL
                     IATSARQIGMFLVILAVVSAAIVAFIIYTLTMDKIREIAVLKLIGTRNRTIAAMILQQ
                     AVALGLIGFVVGKIAATWSAPFFPKYVLLVPADTVAGFAAVMAICVLASLVSIRLALR
                     VDPAEAIGG"
     gene            5449..6183
                     /locus_tag="PKN25_02375"
     CDS             5449..6183
                     /locus_tag="PKN25_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV89772.1"
                     /translation="MDRPTRAGKGILIEGLTKRYGKGDTAVDALKGVDMRVAPGEVVG
                     LIGPSGSGKSTLLKCLGAVIDPTAGRMTLGEECIFDNGWKVRDLRALRRDRIGFVFQA
                     PYLIPFLDVTDNVALLPMLAGVPNAAARARAQELLSALDISHRAAAMPAQLSGGEQQR
                     VAIARGLVNRPPVILADEPTAPLDSVRAMAVVRILNDMAAQFQTAIIVVTHDEKIIPT
                     FKRIYHIRDGVTHEEAGEGRLVRESA"
     gene            6341..6838
                     /locus_tag="PKN25_02380"
     CDS             6341..6838
                     /locus_tag="PKN25_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89773.1"
                     /translation="MQSRSTPRLLAFGAVAILAGLLAAAPATAADSHRHDTHTAVLQL
                     DQGARWQTDAALRTGMEAIRDALARAIPQVHAGRFDAAQYRALAEGVEGQVAYIVQNC
                     KLKPEADEVLHAIIGRLGQGVEVIGGHAAGTKPEEGVVHLAQTLDDYAAHFDHPGWKG
                     LETGH"
     gene            6887..7483
                     /locus_tag="PKN25_02385"
     CDS             6887..7483
                     /locus_tag="PKN25_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV89774.1"
                     /translation="MIGERPSSGADAGADTGEVRELARARAGDVAAFGALVRRHQERV
                     FRFLHRMLDAREEAMELTQDVFVKAWQALPDWRPEAAFSTWLLQIARNAALDQLRRRQ
                     LVRFAPLEEGLEVADDAPGPEARYASRQRQAQLERALQRLAAEHREILLLREVEGLAY
                     GELASVLGIAEGTVKSRLARARSALLEHFDPHTGASHE"
     gene            7548..8015
                     /locus_tag="PKN25_02390"
     CDS             7548..8015
                     /locus_tag="PKN25_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNV89775.1"
                     /translation="MDAALPSEAGAGLLAHCAGCPRCTRRLAELRALGDAFAALPAIR
                     LEVDLAAQLVDRLQAGAPAAARKRGQGPWWVRWMDRIRPPTPWRFAFSPFAGSIAAVG
                     LGLWLGGMLMELEEPVSPPAANTTLAAMSVFGSVPPGNLCPRAGACGASGNAR"
     gene            8012..8476
                     /locus_tag="PKN25_02395"
     CDS             8012..8476
                     /locus_tag="PKN25_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HNV89776.1"
                     /translation="MKTTKPGMALLLSVALNVGVLGAVLLDRLGPGASSTSAPPLHRI
                     LELDEAQRARWDAVEAPFLQQFHAASGQLEAHRRALVEALFAEAIDPLRIETARAAIA
                     ELQQDQQRLIIEQLIAEREILDARQRQQLLGLLLAQPPARTTVEDLHARPTR"
     gene            complement(8473..9801)
                     /locus_tag="PKN25_02400"
     CDS             complement(8473..9801)
                     /locus_tag="PKN25_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNV89777.1"
                     /translation="MKRFLSAFIALVGLAWGVEMLSATGAVASAPGNPWAVREHALTL
                     TGLGSFALMSLAMVLATRPARLERFFGGMDRIYRVHKWAGILAVGFAVLHWLVELSDD
                     LIKTLWGREGRLPKDHGGGLLEAMRDVAEELGEFAIYALLAMLVLTLWKRFPFRIWRY
                     VHKGMPVLYLALAFHAAFLAPLDYWTQPVGVLLAGLIAAGSVASVRVLGGRIGQDRRV
                     GGVVESVRDAGAGVTEVVCRLDEGWRGHRPGQFAFATFDRLEGAHPFTIAGADRGDRR
                     VRFQIKALGDYTRGLAQRLQPGRPMQVEGPYGCFDLPQSLDGREQIWIAGGIGVTPFL
                     AWLEALQARPEQASDVDFYYSVREHATDPFVARLQALCANLPAVRLHVISGARNERLT
                     AATLRDRPRAGAAPEIWFCGPRGLATSLRKGLRGLGMGGASFHQEAFEMR"
     gene            complement(9817..10308)
                     /locus_tag="PKN25_02405"
     CDS             complement(9817..10308)
                     /locus_tag="PKN25_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HNV89778.1"
                     /translation="MKTPALRPLTLGAAAIAVTLMLLGRAQAGDDDFYPPVTDPLTVQ
                     ECASCHIAFPASMLPAASWTRMMGELENHFGDDASVDADTTAAITRYLVDHAGDARGQ
                     PGKLLRGLRPGDAPLRITELPKWVREHDEVPRSEWTRKDVGSKANCTACHIDAAKGYF
                     EDD"
     gene            complement(10330..10746)
                     /locus_tag="PKN25_02410"
     CDS             complement(10330..10746)
                     /locus_tag="PKN25_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HNV89779.1"
                     /translation="MNSQFRAFALLCGLAASAGALAAPSAALLERYAAEARATDPAFA
                     GFSAARGEALHRTRHGLGKPDTPACTSCHGENARSAGQTRTGKAIEPMAVSVSATRYG
                     DPAKVEKWFKRNCNEVLGRECSAREKGDWLAWMSTQ"
     gene            complement(10922..12262)
                     /locus_tag="PKN25_02415"
     CDS             complement(10922..12262)
                     /locus_tag="PKN25_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNV89780.1"
                     /translation="MKNIRFAYAGLLLLLSLLWWMADPLLPAGYEYFALRTVAINYTG
                     VIGIGVMSVGMILALRPVRVEPLLDGLDKTYRLHKWLGITGLVFSVIHWLWAQGTKWA
                     VGWGWLVKPERGPKPPLTDPVESFFRAQRGLAESVGEWAFYAAVVLIVIALAKRFPYR
                     LFFKTHRLLALAYLALVFHSVLLTPFAYWTQPMGIVLAVLMAGGSVAAVVSLSGRVGH
                     RRKAVGEIEELVPFPDNRVLKVAIRFKDRWPGHEAGQFAFVTFSRDEGAHPFTISSGW
                     KEDGRLFFLIKGIGDYTARLPELLRVGDLVTVEGPYGRFDFASGSASGKRRQIWVGGG
                     IGITPFIARMKALAVEPDGREVDLFYSTAEPDQGFIARLQRDAQAAGVRLHVLVSARD
                     GRLDVARICDTVPGWAEADVWFCGPGGFGQSLREGFVARGLPAADFHQELFDMR"
     gene            12252..12440
                     /locus_tag="PKN25_02420"
     CDS             12252..12440
                     /locus_tag="PKN25_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89781.1"
                     /translation="MFFIVLSLGSHFGARGKARPARRVERHLRRVSAKSQRPALSPRK
                     AAAHRMATPFWGWPFRSG"
     gene            12489..12941
                     /locus_tag="PKN25_02425"
     CDS             12489..12941
                     /locus_tag="PKN25_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89782.1"
                     /translation="MMTKSLISTLLLALAAGIATHARAAGDEPTALSPQACQALHQEL
                     DAVAAHVREARASGAAHADAPDPYPALAQALEAHLDALKAGLPTPATPRAQAGELLSD
                     MRDALVLVRGATHLEARLLAVQRLEDDRRLYNRVLETLGCSATRPTAL"
     gene            12946..13863
                     /locus_tag="PKN25_02430"
     CDS             12946..13863
                     /locus_tag="PKN25_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNV89783.1"
                     /translation="MTSITPRQALLLSLAAALSTIAMKTGAWWLTGSIGYLSDALESV
                     VNLAGAGFALWMVSYAQRPADEDHQYGHGKAEYFSAAFEGGLIFIAALIILITAGERL
                     LHPQAIGDLGLGTLLSVGASLINFLVARVLLQVGRAHRSLALEADARHLMADVWTTAG
                     VVCGVGLAGATGWSWLDPVVAGAVALNILREGWELMRRSIDGLMDRALSETEVAAIER
                     TLAEASPPGCFHGRLATRAAGALHFAHVELCVPGEWTVAEAHAVADALEGIVLERTGT
                     RLSTHLEPLPPNLPQGAGVDLGRVPDEGP"
     gene            13887..14555
                     /locus_tag="PKN25_02435"
     CDS             13887..14555
                     /locus_tag="PKN25_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV89784.1"
                     /translation="MHLLIIEDDRTIAENLYEYLEARGHQCDYAGSIAAAAALLACTA
                     FDALVLDRNLPDGDGLALARRLRAEGRAMPILVLTARDALEDKLLGFEAGGDDYLVKP
                     FALQELEVRLLALARRSAVRPADPVLRHGALEFRAATQELRLQGRLLALPPKPLRLVA
                     ELLAQPERVFSRRELEIAVWGHEQDSSDNLRSVLHTLRRTLGDDASTQVVNVHGLGYK
                     LVSR"
     gene            14599..15843
                     /locus_tag="PKN25_02440"
     CDS             14599..15843
                     /locus_tag="PKN25_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNV89785.1"
                     /translation="MRIRIAVSVTAVLVVLIVAQSLALVRLNEEMEEEFIDGTLEEQL
                     HYSIEHSRRLGTLIGPQTPNMTLYRFAPGALPPQGLSPALAGLAVGNHEEWSAGRELH
                     VAVREADGQRYVLVYDESEHRKRESAVVATVVIGGLVLSALSFLLVYAIAARLTRGLE
                     TLAERVEEGGDGTPFAEDGLEAELRVVARALDRAEARQAAVLARERDFNANLSHELRT
                     PLAGIRSDAEMLLNDPVLSDKARRRAERIIATTDRTTTLAQSLLLLAREARPQALEPI
                     DLSAAVRDAWTRLHPAGAAAAAPSIRIDPQAELVADPGLLALVLRNLLENALRHGEGR
                     AIEVVLEGRRLEVCDRGPGFGDEDPARCFERFRRGGAKPGHGLGLALVRHICTACGWE
                     ARAANRPDGGACLTIDFGPASR"
     gene            15957..17705
                     /locus_tag="PKN25_02445"
     CDS             15957..17705
                     /locus_tag="PKN25_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HNV89786.1"
                     /translation="MFKLRLSHVPSFAPSAGASTPVGRRALGASAFAAAWSGARSWRP
                     EVSGEGLLVGLGLYFALACNTPFWRALLASRGGEGGGLFYVLALGVALAALNVALLAP
                     LLNRWTTKPLLGGLILVAAVSSYYAGHFGVYFDPSMLRNVLRTDLAEARELLTPGFFL
                     QVAALALPPLVFLARARVRRRPLRRALAIRAAAAVLALLVAVAALGSVFKDFSGQMRN
                     HKELRYLITPAAPLWSLARVLSRDAQAANQPRRPVGADARLGASWAAAKKPTLFVIVV
                     GETARAANWGLDRGAGQSPAHDTTPELARRAVINFPDVTSCGTNTEVSVPCMFSLQGR
                     RNYDEDAIRGSESLLDVLRHAGLRVVWNDNQSGCKGVCAGVESLRPDPAALPALCDGE
                     RCLDEALLESSRALLRDPQGNLVLVLHQLGNHGPAYFRRYPEAFRRFTPTCDDEDLSK
                     CTREQVVNSYDNALSYTDHVLARGIDLLKELEPRYDAALLYVSDHGESLGENGLYLHG
                     LPYSIAPAEQTRVPMLMWLSSGFAARNRVDAACLRGQAARPASHDNLFHTVLGLLDVR
                     TAVRDDALDLTAPCRS"
     gene            17710..18456
                     /locus_tag="PKN25_02450"
     CDS             17710..18456
                     /locus_tag="PKN25_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNV89787.1"
                     /translation="MHASELFTPLPVPALDASRRWLLAQSALLFLGALLLYGTFERTD
                     LDRRLAHALFDAELGLFPLRHSWFLEAVMHKAAKQATYVLVAASLYLCWQGWRGRLAW
                     LPARNALLAAIGMLAIPLATSTAKLLTARYCPWDLIDFGGYAPYLGLFEAAPPGLKAG
                     QCFPAGHASTGFLWIVWAVALKPAGPRAARAALVAGVLAGGVLGTARMLQGAHFLSHT
                     LWTLWLAWAVSVSLAVLVRADLGLAGRRGA"
     gene            complement(18493..19050)
                     /locus_tag="PKN25_02455"
     CDS             complement(18493..19050)
                     /locus_tag="PKN25_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNV89788.1"
                     /translation="MRPPDSELALRVWDPFVRVFHWSLVSCVILNFFVVDDGETLHQF
                     VGYTASGLVVARLVWGFVGSEYARFANFFPTPVRLRAHLAAMRTGRRDFQPGHNPLGA
                     LMMLALMALVLALGLTGFLQTTDAFWGEEWLEEIHEALAATLIALAGIHAAAAIVMGR
                     LERVNLVGAMITGIKRTRGGRVDLR"
     gene            complement(19114..19371)
                     /locus_tag="PKN25_02460"
     CDS             complement(19114..19371)
                     /locus_tag="PKN25_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNV89789.1"
                     /translation="MNRILALAAAALVSTSALAGPTCTAPKEKWMKEADFKAKVEAQG
                     YKIKNFKVTKNDCYEIYGFDKDGKKVEIYFNPETGAELERK"
     gene            19571..20239
                     /locus_tag="PKN25_02465"
     CDS             19571..20239
                     /locus_tag="PKN25_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HNV89790.1"
                     /translation="MDLPWLDHPARPAPGAQPAARSLRLLEVDDARRPAVEHFVRERF
                     AAHYQARVRHFMPRLFALEAADGSLHGAVGCRGAADQGLFLERYLDEPVERVITRRAG
                     ERVARDGIVEVGNLAAHGAGTARLLIIALTRILAAEGLRWVCFTGTPALINSFRRLGL
                     DPLTLGPADPRRMGAELPEWGSYYDAGPLVMAGEIRGGDRTLAGAGRELQSGRGGQLR
                     AGCV"
     gene            20223..21743
                     /locus_tag="PKN25_02470"
     CDS             20223..21743
                     /locus_tag="PKN25_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV89791.1"
                     /translation="MPDASESFLAGLARHGGRPALRGESGEIAFDRLPEAVAACRGVL
                     ESVAARRVALALDNGLQWALWDLALLADGRVCVPLPGFFSPAQQAHVLDSAGVDTLIV
                     DPVAASASAAVFPGFVPVAPGILRRVPAQVPALPTGTVKITYTSGTTGQPKGVCLSAA
                     AQLAVARSVARIGEAGAVERHLGVLPLATLLENIAGLYAGLLAGACVELLPMRTIGFS
                     GGGGFDPARFLQTLHARRPHSLILLPQMLLALVGAAEQGHAPPAGLRFVAVGGGQVSA
                     RLLQRAEALGLPVYEGYGLSECASVVCLNTPAARRVGTVGRPLPHAALRIADDGEVQV
                     RGAHMLGYLGEAPLADQLVAGEWLGTGDLGHFDDGFLVLHGRRKHQFITAYGRNVNPE
                     WVEAELVQQGPIAQAWVHGEALAENLAVLVPRRADCSDAELDAAVAAANAGLPDYARA
                     GRWLRADAPFTPANGLLTANGRLRRAALAAHYLPRSGADGAASCATFTSFSDEIDT"
     gene            21740..22393
                     /locus_tag="PKN25_02475"
     CDS             21740..22393
                     /locus_tag="PKN25_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNV89792.1"
                     /translation="MSFFQQLQDQTAAERETLFAAPIIQQALAGKVALPSYVAFLTEA
                     YHHVSHTVPLLMACGARLPARLEWLRGAIAEYIDEEYGHQEWILNDLAACGADPEAVR
                     RSQPALPTELMLAYVYDRIARHNPVSFFGMVNVLEGTSIALATRAAGTIRSSLGLPPE
                     AFSYLNSHGSLDLEHMDFFATLMNRLDEDDDRAAVVHTARVVYRLYGDIFRSLPLAA"
     gene            22405..23211
                     /locus_tag="PKN25_02480"
     CDS             22405..23211
                     /locus_tag="PKN25_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV89793.1"
                     /translation="MELNGTRVLVTGASGGIGQALVEQLCAAGAQVLAVGRDADRLAD
                     AQERFPQALRRVVADLATIAGVDAVAAAARDLGGVDCVVQAAGLNRFGLVEQQDDDAI
                     ATQVALNLVAPMLLTRRLLPLLRERPRALLLFVGSTFGAIGYPGFAPYSATKFGLRGF
                     AEALRRELADGPVRVLYLAPRATRTAMNASAVVEMNRALGVAMDPPQQVAAAIVQALR
                     DESPERHLGWPEKFFARLNRLLPRVVDRALRGQLATIRRFAHSESEEIRS"
     gene            23208..23924
                     /locus_tag="PKN25_02485"
     CDS             23208..23924
                     /locus_tag="PKN25_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV89794.1"
                     /translation="MKMPAQETFASGTLRLARRVALPLAFPLLSLVAAPAAFALADAG
                     KLELQAVQGRWAEIHYGLPEKQREAAFAELAERAARAVAAEPEAAELRIWHGIVLSTL
                     AGAKGGLGALGLVKEAKAELETALRLDPQALDGSAYTSLGSLYYQVPGWPVGFGDDAQ
                     AEKLLKQALAINPGGIDPNYFYGDFLARQKRYAEARTALERALAAPDRPGRASADAGR
                     RAEARRLLEQVAAKLAQGAQ"
     gene            23986..24645
                     /locus_tag="PKN25_02490"
     CDS             23986..24645
                     /locus_tag="PKN25_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV89795.1"
                     /translation="MRILLVEDDAALGEGIRVALKPEAYTVDWVQDGASALHALTHED
                     FALAVLDLGLPRMDGLQVLQRLRAAANPVPVLVLTARDATSDRIAGLDAGADDYLVKP
                     FDVDELKARIRALLRRSANRAQPVLHHGEVVLDPVNQQVSYRGQPVVLQRKEFLLLHE
                     LLTQPGRVLTRDRLQQALYGWGEEAESNTLEVHIHHLRRKLFPELIRTVRGVGYLADK
                     A"
     gene            24645..26189
                     /locus_tag="PKN25_02495"
     CDS             24645..26189
                     /locus_tag="PKN25_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV89796.1"
                     /translation="MGSIRRRTLLLVLGLLGVALGAISYLGYRDARHEVRELFDARLA
                     QQARLLAGMIPGGMAPDARAALQDALDAGGLGAAVGEGTDAEREDGDEALPLGHEYEG
                     KLGFVVVDDQGLALLQSAAAPMGALDLLLGARTRGAEQQGLGEVGEDLAGYHTVSLHD
                     GAWRLFLLRDARDRQWILVGEREDVRGELLGRITLRSVLPDLVGLPLVAVLVWLAIGW
                     GLRPLARIVESLQARGPDDLSALALQDVPQELEPMVAALDRLLHQVNELLERERRFLA
                     YAAHELRTPLAVLRIHAQNALQAPDPADREEALRLLGSGIERATRVVAQLLTLARLEP
                     DASRPKRLPIELLALVREQLAELTPLADEHGQDLALEADEGADFHLLGDAGSLGILMQ
                     NLVGNAVRHTPPDGCIRVLLEATPAAIVLRVQDSGHGVPPELREKVFERFFRAGGGQG
                     AGLGLAIVARIVELHGGTIALDGCALGGLEVRVVLPRDAAAPRRVQGDEGKVPPCSAS
                     GGAARP"
     gene            26314..26832
                     /locus_tag="PKN25_02500"
     CDS             26314..26832
                     /locus_tag="PKN25_02500"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNV89797.1"
                     /translation="MPSNEDFHAGERALQQRWGTASLWDAARRERLLWDHLPDWVQPR
                     LAAAPFFFLATSDATGRCDCSFKGGGPGLVRILDARRLAFPDFDGNGAFMSLGNLRQN
                     PQVGCLFIDFGDGARLRVNGRATLLEGEAAARLFPGQDRAVVVDVEQVVPNCARHVPT
                     LHPPTADARGAA"
     gene            26829..27776
                     /locus_tag="PKN25_02505"
     CDS             26829..27776
                     /locus_tag="PKN25_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV89798.1"
                     /translation="MKIPYRIAANVEWTSKCNAACAMCPREAIDRPQSMSMATWRQVL
                     DRLAPDEIFRVVLAGYGEPTTHPRFFEFIDELRPHPVRFDMVSNGHLLDVERLRHLDG
                     AIDLLMVSFSSIDPDVYARVHVNLDQRRVMDNLQAASRVLRRTRLAISLTPMPDCLPA
                     LGDTIAWLRAHGIERLTMSPTLYNRGGKLEAHRLASAELRRMIRQHGLRSQELDFVPS
                     AAEIFRQWRRNRFGCIPRNVDLFVAASGDYLYCYNDVAHRHPIGHVAADPVAAVLRRR
                     EGMAALPTLCEGCNMRHRYGPRELLRAGVGFARARLREG"
     gene            27949..>28367
                     /locus_tag="PKN25_02510"
     CDS             27949..>28367
                     /locus_tag="PKN25_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004378294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89799.1"
                     /translation="MKTRTLMATAMGVLCAGLTFAQQPVAAPGEEAPVFGAQIMTEQE
                     RVEHRERMRAAGTPEEREQLRLEHHKQMVERARERGVTLPDEPPMRGGMGAGGGMGAG
                     AGMGAGGGMGPGGGRGPGAGMGLGGGMGPGGRMGSGAG"
     assembly_gap    28368..28467
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            28887..30068
                     /locus_tag="PKN25_02515"
     CDS             28887..30068
                     /locus_tag="PKN25_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004384132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV89800.1"
                     /translation="MKKNLWLLAIAQGLFLTNNVVFIAINGLVGFSLAPVGWMATLPV
                     MGYVVGGALSTGLVARSQRRWGRQVSFQLGLLVAFLTALLCAFALSLGSFWLLVAGTV
                     VAGYYSANGQLYRFAAGELAPSSFREKAVSLVLAGGLIGAVIGPNLANHTREAMAVPF
                     LGSYLALAVVALVSMAVMAGIRFPPQPVKKAGAEGGRPLAEILRQPVFIVSVLAAAMS
                     YGVMNLLMAATPLAMDVCGMPFSDAALVLEWHVIGMFAPGFFTGHLIKRFGVLRIMGV
                     GVVLNFACVAVALSGQDLHQFLLALFLLGVGWNFLFTGATTLSMEAYRPEEKDKAQAA
                     INFVVFAVMAFTSFASGALVTTQGWNLLNVGSIAPLALTAAALGWLALLRRRAADGQG
                     A"
     gene            30065..30688
                     /locus_tag="PKN25_02520"
     CDS             30065..30688
                     /locus_tag="PKN25_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HNV89801.1"
                     /translation="MNFLAHAWLAGESPALVVGGVFGDWVKGTLPGALPADLARGVAL
                     HRAIDAFAETHPAFRASRARVSAARRRYAGVLVDIFWDHLLARDWAEHGEGALASYCA
                     GVYRQLAARRADLPASAHLALERMAREDWLGSYAGLEGIADVLARMGRRARQPNPLAG
                     AEVEFVADPAGFAQDFADWVPDARRFVAEWRDGRARGGRRAGLNDAA"
     gene            complement(30761..31009)
                     /locus_tag="PKN25_02525"
     CDS             complement(30761..31009)
                     /locus_tag="PKN25_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89802.1"
                     /translation="MNAHKIDDPFGFREHPGVYDTGTGAIKTVEANRGIPGIERVVIR
                     SYCGRTQDNRVFYRLSADRSREFATLAEAFAARPVHLT"
     gene            complement(31084..34311)
                     /locus_tag="PKN25_02530"
     CDS             complement(31084..34311)
                     /locus_tag="PKN25_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HNV89803.1"
                     /translation="MLPAYAELHCLSNFSFQRGASHPEELVAQAAAFGYSAIALTDEC
                     SFAGVVRAHRALQALPEAGRPRLIVGCELRLADGPCLVLLAADRAGYGRLSRLLTQAR
                     RSADKGRYHLTRAMLDATRADEAAAEALPGCLALLIPPEEFPPPGAAGDAAIALRSEA
                     RWLAKRFPGRSWIAHTPRLDGRDPARLHWILRIAQATGVPAVAAGGALMHEAARRPLA
                     DVMSALRLHCSVAEAGLALAPNAELRLHERATLARRHPPELLAETVAVAARCRFDLDE
                     LRYEYPAELVPADETPTRWLRRLVEGGLAWRYGKAGKERRAAAPATIAAAPPPAEDPA
                     PPTVRAQIEHELALIAELGYEPYFLTVHDIVRFARERGILCQGRGSAANSVVCWALGI
                     TEVDPQLGIMLVERFISRERDEPPDIDVDFEHERREEVIQYLYRKYGRERAALAATVI
                     RYRARSALRDVGRALGLDETQIERLTREHHWFDGRHILPERLAEAGLDPASPVTQRLV
                     ALTEELIGFPRHLSQHVGGFVIARGRLDELVPVENAAMSDRTVIQWDKDDLDAVGLMK
                     IDVLALGMLSALRRGLALVSAWRGEALTLATIPRERKEVYEMLSRADSVGVFQVESRA
                     QMTMLPRLRPQRFYDLVVEVAIVRPGPIQGGMVHPYLQARERAARGEDPLDGLREEIR
                     GVLARTLGVPIFQEQVMQLAVVAADFTGGEADQLRRAMGAWRRKGELERYRQKLLDGL
                     ANNGYDPDFAQRLCQQIEGFGSYGFPESHAASFALLVYASAWLKCFAPAAFLAALLNS
                     QPMGFYAPAQLIQDARRHGVEVRAADVGASAWDCMLEAADGAQPAVRLGLRMIRGLGR
                     EAATRIAAARGEHAFADTQDLAARARLDTGELRTLAAGGALASLAGHRRQALWQASGA
                     APLPGLLAEAPGGDVAATLDAPAEAEDLLADYARLGFTLGRHPLAFVREQLARLRFLT
                     AADITAAPDRMLARGAGLVTCRQRPGTAKGTLFLTLEDETGLTNVIVRPELFEQQRRI
                     LLGARLMGVFGQIRRQGRVVHLVASRVVDHSPLLGSLAARSRDFH"
     gene            34507..35478
                     /locus_tag="PKN25_02535"
     CDS             34507..35478
                     /locus_tag="PKN25_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV89804.1"
                     /translation="MSFPLSRKVLFGALALAFATAMPAYAQQKSVAITAIVEHPALDS
                     VRDGVKEALAAAGYEDGKNLKWQYQSAQGNTGTAAQIARKFVGDKADVIVAIATPSAQ
                     AVAAATKDIPLVFSAVTDPVVAKLVPSMQPSGTNVTGVSDALELGKQVELIKRVVPAA
                     KRVGIVYNPGEANSVVVVEQLRELLPKHGLSLVEAAAPRSVDVGSAARSLIGKADVFY
                     TSTDNNVVSAYEALVKVGMDAKIPLVAADTDSVARGAVAAYGMDYKALGVQTGEIVVR
                     ILKGEKPGAIASETSNKLSLQVNPAAAQKQGITLAEDLVKSAAKVVQ"
     gene            35577..36467
                     /locus_tag="PKN25_02540"
     CDS             35577..36467
                     /locus_tag="PKN25_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV89805.1"
                     /translation="MSLYTLLGALEIGLIFSLVALGVYISFRLLRFPDLTVDGSFPLG
                     GAVAATMIAAGHDPFTATIAATLAGAIAGLITGWLNVSLKIMDLLASILMMIALYSIN
                     LRIMGKPNVPLITEPTVFNLLQPEALADYVFRPLLLVFVVLAVKLALDGFFATQSGLA
                     IRATGSNARMARAQGVNTGMAILAGMALSNALVGLAGALFAQTQGGADISMGVGTIVI
                     GLAAVIVGESLLPSRKLYLATIAVIVGAIVYRFFIALALNSDFIGLQAQDLNLVTAVL
                     VTVALVVPRLRAHWSGKRVS"
     gene            36471..37265
                     /locus_tag="PKN25_02545"
     CDS             36471..37265
                     /locus_tag="PKN25_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV89806.1"
                     /translation="MLKATDLRLTFNPGTPIETQALRGMSLEIPTGQFVTVIGSNGAG
                     KSTFLNAVSGEQRVDSGTIEIDGIDMTRQPVWGRARHVARVFQDPLAGTCEDLTIEEN
                     MALAKMRGERRGFSFAVKPALREEFRAQIATLGLGLENRLSDRIGLLSGGQRQAVSLL
                     MAALQPSRLLLLDEHTAALDPRTAAFVLELTVRIVAEHKLTTMMVTHSMRQALDVGQR
                     TVMLHQGRVVLDVSGKEREGLDVHHLLDMFEKVRGEKIDDDALLLG"
     gene            37381..38799
                     /gene="gltX"
                     /locus_tag="PKN25_02550"
     CDS             37381..38799
                     /gene="gltX"
                     /locus_tag="PKN25_02550"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNV89807.1"
                     /translation="MTSASRPVRTPVRTRFAPSPTGYLHIGGARTALFSWAFARRHGG
                     TFILRIEDTDVARSTPAAVQAILDGMQWLGLEHDEGPFYQMQRMDRYKEVIGQMLAAG
                     TAYHCYLSPEELDRLREAQRERGEKPRYDGRWRPEAGKVLPQPPAGVQPVVRFRNPAE
                     GVVSWDDLVKGRIEIANAEMDDFIIARADGTPTYNFCVVVDDWDMGITHVIRGDDHVN
                     NTPRQINVLQALGAEVPLYAHLSMILGDDGTKLSKRHGAVSVMQYDEEGYLPEAVINY
                     LARLGWSHGDDEIFDRRQFVEWFDLDHITPSAAQFNTEKLNWLNAQYIKKADDAFLAA
                     EVASRLARREVNPETGPSLEAVVALYKDRVANLNELADAAELFVADVHPAPEVVGQHL
                     TDVAKAALASLRGRLAAAEWNRAALSQAIKDTMAEHGLKMPQVAIPLRVAVLGVPKTP
                     AIDAVLEVLGRGRVLERLDRRL"
     gene            complement(39139..39579)
                     /locus_tag="PKN25_02555"
     CDS             complement(39139..39579)
                     /locus_tag="PKN25_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNV89808.1"
                     /translation="MNTHPAFQQFAHSTRNGFEQFQAYALIALDASEKLCALNFSAAR
                     ALCEAASAAPIAQPGSDLEAGFAKQRETQGQALEQLGNYLQQVNEVVSGAQNELLELG
                     SRHFEDFQASMQSMLEQARKLGGVEVVQSEAAEEPRSRAIRKAA"
     gene            complement(39715..40377)
                     /locus_tag="PKN25_02560"
     CDS             complement(39715..40377)
                     /locus_tag="PKN25_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV89809.1"
                     /translation="MSLRILLADDHQMFRHALRALLARDPELEVVAEAASGEEVLAIA
                     DAQPVDLVCMDIAMPGMDGIEATRRLLARHPALRVIGLSAFADRQFVTDLLAAGARGY
                     ITKGEAGDELLRAIRTVCEGRSYLCPEVAATVADALRDSPGVYAAAPRLTARERQVLQ
                     LIAEGLTSVQIGDRLHIAASTVEVHRRNLMRKLDLHNVAELTRYAIHSGISPNVTPGG
                     AG"
     gene            complement(40374..41498)
                     /locus_tag="PKN25_02565"
     CDS             complement(40374..41498)
                     /locus_tag="PKN25_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV89810.1"
                     /translation="MRSPRLEALRSALEKAEFRLAATQDLARVGDWELDRHTGRMYWS
                     RELFRLFERPEALGVPDLNEALGYFSLESTNRTRDLFWEAIDSGRRCALEQEVLLPSG
                     EERRHFTVIVPVADETGRVFRLYGTVQDITERRRLESERLEHLERLEALSRHLVEIEE
                     RERRELASALHDRASPNLAALQILFSSLADALPESARDELAPLLEDASALLADTTAGI
                     REICTNLRPATLDYAGLVPALREYVAQFRARTGLDVRVDAASGSPPCALSRATQTLCF
                     RLVQEALTNCAKHARAGSVRIGLGGCAGGVLLQIGDDGVGFDLSRLGEAGSTPGLGLI
                     TMRERVELAGGDFRLHTRPGDGTVIEVRLPAELHPAETTR"
     gene            complement(41495..43429)
                     /gene="mnmC"
                     /locus_tag="PKN25_02570"
     CDS             complement(41495..43429)
                     /gene="mnmC"
                     /locus_tag="PKN25_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HNV89811.1"
                     /translation="MPIHPARLERTPEGVLYSGAFGDVYHSRDNALGQAHAVFIAGNG
                     LPQRWQGRERFTIVETGFGAGLNFLATWAAWREDACRSARLHFVSCELHPFCREDLAR
                     IHAAWPELATFAAELRAQWPVLAPGLHRLNLDAGRVTLTLFLGDAADGLARIDARADA
                     LYLDGFAPAKNPALWSERVFHLLAGLAAPDATLATWSVAGQVREGLRRAGFTTEKAPG
                     FGTKWQMLRGRLAREARPLAVPEAATSGRHALVLGAGIAGCTVAERLAGRGWTVELID
                     RAPAPATGASGNLAGVLRPLPSLDDNRMSRLTRAGSLYAWRRIHQLRVRGLALRADDC
                     GVLHLGRDAAQEVKMRAVVERLALPPEHLRFVGVDEAAEIAGCAVANGGWWFAGSGWV
                     QPPSLCAASLEAAGGRLRGHFGHTVARLGYDGGAWHAHGPDGSTIASAPVAILAPGTG
                     IADFEQASLPVVSARGQVSHLPAVAGSAPRVVVCRGGYVSPAVDGLRCAGATFAVDDP
                     DPGLRPQDHAENLQRLEAMIPGFGAGLGAGFDPDRLDGRVGFRPASPDRLPMVGALPL
                     PDRPPASAARGLDELPRRPGLYALSGYGARGLVWSVLAAELLASALEGDPAPLERDLV
                     EAIDPARFVLRPLARTAVRE"
     gene            complement(43494..>44177)
                     /locus_tag="PKN25_02575"
     CDS             complement(43494..>44177)
                     /locus_tag="PKN25_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNV89812.1"
                     /translation="TLMDFTEGLIRHAAREALGTESFVYQGRELDLSKPFHRLTIVQA
                     IRKYHPGFTAEQLADAAWLKEKIVAFGEKVKPGGLGSLQLQLFEACAEAELWEPTFII
                     DYPVEVSPLARASDTDPEITERFELFIVGREIANGFSELNDPEDQAARFHAQVEAKEA
                     GDEEAMYYDADYIRALEYGLPPTGGCGIGIDRLVMLLTDAPAIRDVILFPQMRPEAAA
                     GLVIPEPEL"
BASE COUNT         6049 a        15412 c        16105 g         6411 t
ORIGIN      
        1 ggcggcgagg gccggacggg ggaatcgcga cttgcgtcgc tcctacggcg cgctgcggag
       61 ccgcggtggt tcttgtggga gcgacgcgag tcgcgatctg cgtgctcgtg ttcagcttgc
      121 attcagattg cggcgggcac actgtctgcg tcgaaacccg ccggagaatg aagatgaaga
      181 cgcgattccg ttccctgctg gccgccgccg cgctccttgt cgcaggtggc ctgtccgtgt
      241 ccggcgcgag cgcgggcgag cgcgtgcgcg tggaggagat ccgccagctg cgcgagagcg
      301 ggcagatcct ctccgccgag gacattctcg cgcgcagccg cgccatccag cctggccagg
      361 tcgtcggtct ggagctcgag cgcgaggacg gacgctggat ctacgaggtc aagctgatcg
      421 acgagcgcaa ccgggtgcac gaactcgagc tcgacgccgc caacggggaa gtgctcgaac
      481 gcaaggagaa gtgagcgatg cgcctgctcg tggtcgagga cgatgcggcg ctcggcgagc
      541 gcctgcgcac gcggctggtc gccgaaggct atgcggtgga cctcgcgcgc gacggcatcg
      601 acggcgccca tctgggcgcg accgagccct acgacgcggt gatcctcgac ctcggcctgc
      661 ccggcaagcc ggggctgacc gtgctgcgcg agtggcgcgc ggcggcgaac cgcgtgccgg
      721 tgctgatcct caccgcgcgc gacggctggg ccgagcgtgt cgagggcctg caggccggcg
      781 ccgacgacta tctcggcaag cccttccatg tcgaggagct cctcgcccgc atccaggccc
      841 tgctgcgccg cgccgcgccg gtgcccgaca ccgagttgcg cgcgggcgac tggcggctgg
      901 acgaggcgcg ccagtgcctg gtcgatcgcg ccggcaacga gcaggccctg accgccaccg
      961 agttccgcct gctgcgctac ttcatgcgcc accccgacga ggtgctgtcg aagacgcggc
     1021 tctccgagca cgtctatgac tacgacgccg agcgcgacag caacgtcatc gaggtctacg
     1081 tccgccgcct gcgcggcctg ctcggcaacg aacgcatcga gacccggcgc gggcagggct
     1141 atgtgttccg cagcggcgac tgaggcgcgt gcgcgggggc gggccggaag atgaactcga
     1201 tccgccgtcg cctctcgctc acgctcgccc tggtgctgct cgccgcgggc gtcctgctgg
     1261 ccttcgcact gcgcgacttt ccgcgccgca tggtcgagga gcacgtgctc gcccgcctcg
     1321 accacgacgc cgacctgctc tacgtgcatc tgctcgacgc gctcgcacgc caggacggcg
     1381 aggcggggat ggacagcgcg ctgatgggcg cggccgggcc ggcctacgac ctgccgctgt
     1441 cggggcatta cttcctcgtg cgcctcggcg atcggcagtg gcgttcgcgc tcgacctggg
     1501 acgccgacct cgacctgccg gagggggcgg aggggcttgt gtcgcctcgc ctggccggcc
     1561 ctgccgggca ggcgctgctg gtggtcgcga agcgcttccc cgacagcgcc gacggccgcg
     1621 cggtgagcat cgcgctcgcc gaggacgtcg ccgcgatcga tgccgcgatc gcggccttcc
     1681 gcgcgcaggc gctgaccgtg ctcggactgg cgctgctcgt gctgctggtg ctgcagcggc
     1741 gcgtgctgct gcgcgggctg gcaccgctgg acgaggcggt ggacgcctgc cggcggctgg
     1801 agcgcggcga ggcggtgcag ttcgaggccg acgcgcccgc cgaggtgcgc ccgctgctcg
     1861 ccgccgtcga gcgcctgtcg cgccatcagg cccagcgcct cggacgcatc cgccacgccg
     1921 ccggcaacct ctcccacgcc ctcaagacgc cgctcgcggt gctcggccac agcgcggacg
     1981 cgcttgcggc gcgtggcgac ggggagggcg cggcggcgat ccgcacccag ctcgagacca
     2041 tgcgcgcgac catcgagcgc gagctgcgcc gtgcccgcct tgccggcggc gctgcgggcg
     2101 gcgccggctt cgcgctgcgc gcccagctcg gcgcgctggt cgaggtgttg cagcgcctgc
     2161 acgccgggcg tggcctgcgc atcgagctgg aggcggacga gcgcacctgg ccggtggatc
     2221 gcgaggacat gctggagctc ttcggcaacc tgctcgacaa cgcctgcaag tgggcgcgtg
     2281 cgcgggtgcg ggtggttgtg gcgcccgacg cggggacggc gcggctcgcc ttcagcgtgg
     2341 aggacgacgg ccccggcgtg gcgcccgagc tgctcgagcg cctcggcacc gccggcctgc
     2401 gcgccgacga gtcccgcccc ggccacggcc tcggcctggc gatcgtcggc gacatcgtca
     2461 cccagtacgg cggcacgctc cgcttcgggc gcggcagcgt gctcggcggc ctgcgcgtgg
     2521 acgtcgagct gccgctggca gtgtaggggc gacccgacgg gtcgcactta cgtcggctac
     2581 ggaaacgtcg tggtccgaat ataagggttt cccgaaggac tttccttagc taagatagta
     2641 agcaattact cactaaagag agcgttgccg tgcgtgccaa acccctcccc gccgagacgc
     2701 gtcgggccgt caccgtcgag gcggtgatcg agctggcggc cgagcgtgat cccggcgaga
     2761 tcaccaccgc ggcgatcgcc agccacatga agctgacgca gggcgcgctg tttcgccact
     2821 tcccgagcaa ggacgcgatc tgggaggcgg tcatggagtg ggtcgccgag cgcctgctgg
     2881 cgcgcgtcga ccgtgccgcg gcgcaggcgg cgtcgccgct cgcggcgctg caggcgatct
     2941 tcctcgccca tgcggacttc gtcgccgagc atccgggcgt gccgcgcatg ctgttcgggg
     3001 agctccagcg tgccgaggcg acgccggcga agcggctcgc gcgcgcgctg ctcgagcgct
     3061 acggcgagcg catccgcggc cgcctcgaag ccggcaaggc ggcaggcgag gtgtcggcgg
     3121 cgctggacat cgatgcggcg gtgatcctct tcgtcggcac cctgcagggg ctgatcatgc
     3181 agtccatgct cgccggcgac ctgctccgga tacggcggga cgcgccgcgc gtcttcgcca
     3241 tctacctggc cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgggcag
     3361 gtgctcggcg agatggacgc ggtggatctc ggcgaccgca tccgcgccca ggaggccgcg
     3421 ctcgagcgcg cgcaggcggc gatgcgcgag gccgaggcgc gccaggcgtt cgcgcgcgcg
     3481 caggcacggc gctacgacaa gctgggcagc ctgcaggccg cgagcgagga gcttgcgctc
     3541 acccgccgcc aggagctcga ggtcgccgag gccggcctcg ccgccgcgcg cgaggagcgc
     3601 acgcgcgccc gcgcggaagg cgccgcgctg caggctcagc tcgccaacct gcgcctggtc
     3661 gcgccggtcg atggcgtggt cacgctgcgc gaggtggagc cgggcaccac ggtcgtggcg
     3721 ggccaggcgg tactcgaggt gatcgacccg gcgaccctgt gggtcgacct gcgcctcgac
     3781 caggtcaatg ccggagggct ggccgccggc ttgccggccc gcgtcgtgct gcgctcgcgc
     3841 agcggcgagg cgctcgccgg gcgggtgctg cgcgtggagc cgaaggccga cgcggtgacc
     3901 gaggagctgc tcgccaaggt ggtcttcgac cggctccccg agccgatgcc gccgctcggc
     3961 gagctggccg aggtcacgct ggcgctgccg ccgctgcacg ccgcggcggt gatccccaac
     4021 gccgcgctgc ggcgcgaggc gggtgcggtc ggggtgtggc ggcgcgcggg ggagggcggt
     4081 ctgcgcttcg tgcccttgaa gctgggcgcg agcgacctcg acgggcgcgt gcaggtgcgc
     4141 gagggcctgg cggcgggcga cgagatcgtc gtgtatagcg aggccgggct gcgcgcaggc
     4201 cgccgcaccg aggtcgtcgc ggcactggac ggagcgggca aatgatcagc ctcgccgggc
     4261 gcgacatcct gcacggctgg ggcaagttcg tgttcaccgg cgtcggcctg gggctgctga
     4321 tcggcgtcac gctggtgatg gcgggcgtgt atcgcggcat ggtcgacgac ggcaaggcct
     4381 tgctcgacaa cagcggcgcc gacctctggg tggtgcagcg cgacacgctc ggcccctatg
     4441 cggagtcgtc cagcgtccac gacgacctct accgcagcct gctcggccag cgtggcgtgg
     4501 cgggcgcggc gaacatcacc tacctgacca tgcaggtgcg caagggcagc gaggacgtgc
     4561 gcgcgatggt ggtcggcatc gccgccggcc ggccgggggc cacgccgggc tggccgccgt
     4621 atctggtcgc ggggcggcag atcacgcgcg ggcattacga ggcggtggcc gacgtcgcca
     4681 gcggcctgcg cctcggcgag cgcgtgggca tccggcgcaa ccagtacacc gtggtcgggc
     4741 tgacccggcg catggtgtcc tcggccggcg acccgatggt gttcatcccg ctcaaggatg
     4801 cgcaggaggc tcagttcctc aaggacaacg acgccatcct gcagggtcgc cgccgcactg
     4861 cggccaaccc ggcgctcaac cgcccgggcg tgcccggcct gctcgacgcg gtgctcgcct
     4921 cgcagagcac caacccctat gtgaatgccg tgctgctcac gctcgcgccc ggccacgcgc
     4981 ccgacgaggt cgccgaatcc atccggcgct ggcagcgcct caccgtgtac acccgggcgc
     5041 agatggaggg catcctcgtc ggcaagctga tcgccacctc ggcacgccag atcggcatgt
     5101 tcctggtcat cctcgcggtg gtgagcgcgg ccatcgtcgc cttcatcatc tacacgctga
     5161 ccatggacaa gatccgcgag atcgcggtgc tcaagctcat cggcacgcgc aaccggacca
     5221 ttgccgcgat gatcctgcag caggcggtgg cgctcgggct gatcggcttc gtcgtcggca
     5281 agatcgccgc cacctggtcg gcgcccttct tccccaagta cgtgctgctg gtgcccgccg
     5341 acacggtggc gggcttcgcg gcggtgatgg cgatctgcgt gctcgccagc ctggtgtcga
     5401 tccggctcgc gctgcgcgtc gatccggccg aagcgatcgg aggctgagat ggaccgaccg
     5461 acacgcgccg gcaagggcat cctgatcgaa ggcctcacca agcgctacgg caagggcgac
     5521 accgcggtgg atgccttgaa gggcgtggac atgcgtgtcg ccccgggcga ggtcgtcggc
     5581 ctgatcggcc cctcgggctc gggcaagagc acgctgctga agtgcctcgg tgcggtcatc
     5641 gaccctacgg cggggcgcat gaccctgggc gaggagtgca tcttcgacaa cggctggaag
     5701 gtgcgcgacc tgcgcgccct gcgccgcgac cgcatcggct tcgtgttcca ggcgccctac
     5761 ctgatcccct tcctcgacgt caccgacaac gtcgccctgc tgcccatgct cgccggcgtg
     5821 ccgaacgccg cggcgcgcgc gcgggcgcag gaattgctga gcgcgctcga catctcgcac
     5881 cgtgccgcgg ccatgcccgc gcagctgtcg ggcggcgagc agcagcgcgt cgccatcgcc
     5941 cgcggcctgg tcaaccgccc gccggtgatc ctcgccgacg agcctaccgc gccgctcgac
     6001 agcgtgcgcg cgatggcggt ggtgcgcatc ctcaacgaca tggccgcgca gttccagacc
     6061 gcgatcatcg tggtgaccca cgacgagaag atcattccca ccttcaagcg catctaccac
     6121 atccgcgacg gggtgacgca cgaggaggcg ggcgaggggc ggctcgtgcg cgaaagcgcg
     6181 tgaggaccgc tgcgcgggcc gccccgccgc gctgcgatgg ggtcgggcgg cacggaatgt
     6241 tcttgcaccg gggcatggaa cattttccgc ggcgcttcgt ttcattgtcc aggaaggcga
     6301 ttccgccctc tcgaaaccat cacgaaagga cctcttcgac atgcaatccc gctccacccc
     6361 ccgtctgctc gccttcggcg ccgtcgcgat cctcgccggc cttcttgcgg ccgctcccgc
     6421 cacggcggcc gacagccatc gccatgacac ccacacggcg gtcctccagc tcgaccaggg
     6481 ggccagatgg caaaccgacg ccgcgctccg gaccggcatg gaggcgatcc gcgacgcgct
     6541 cgcgcgggcc atcccgcagg tgcatgcggg gcgcttcgat gcggcgcagt atcgtgcgct
     6601 ggccgaaggg gtcgaagggc aggtcgccta catcgtgcag aactgcaagc tgaagcctga
     6661 ggcggacgag gtgctgcacg cgatcatcgg caggctcggc cagggcgtcg aggtgatcgg
     6721 cggccacgcc gccggaacga agccggagga gggcgtcgtc cacctcgcgc agaccctcga
     6781 cgactacgcc gcgcacttcg accacccggg ctggaaaggg ctggagaccg gacactgatt
     6841 cgacagcggc agcggaggaa ggcctggtga cggatctccc gcgcgcatga tcggcgagcg
     6901 cccgagcagc ggggcggacg cgggcgcgga cacgggtgag gtgcgcgagc tcgcccgcgc
     6961 ccgcgccggc gacgtggccg ccttcggcgc gctcgtccgc cgccatcagg agcgcgtgtt
     7021 ccgcttcctc caccgcatgc tcgatgcccg cgaagaggcg atggagctga cccaggacgt
     7081 cttcgtcaag gcctggcagg ccttgcccga ctggcgcccc gaggcggcgt tctccacctg
     7141 gctgctgcag atcgcgcgca atgccgcgct cgaccagttg cggcgccggc agctcgtgcg
     7201 cttcgcgccg ctggaggaag gcctcgaggt cgccgacgac gctccgggac cggaggcccg
     7261 ctatgccagc cgacaacgcc aggcgcaact ggagcgggcg ctgcagcggc ttgccgccga
     7321 gcaccgcgag atcctgctgc tgcgcgaggt cgagggcctg gcctacggcg agctggccag
     7381 cgtcctcggc attgccgagg gcacggtgaa atcccggctc gcacgcgcac gcagcgcctt
     7441 gctcgaacac ttcgacccgc acaccggagc ctcccatgaa tagctcttgc cccacaggat
     7501 cttgtgcccc cgggccgtgt cctcccgacg aggcgctgtc ggcgctcgtg gatgcggccc
     7561 tgccctccga ggccggcgcc ggactgctcg cgcactgcgc gggctgcccg cgctgcacgc
     7621 gccggctcgc cgaattgcgc gccctcggcg acgccttcgc ggccctcccc gccatccgcc
     7681 tcgaggtcga tctggccgcg cagcttgtcg accgcctgca ggcgggcgcc ccggcggccg
     7741 cgcgcaagcg cgggcagggc ccctggtggg tgcgctggat ggatcgtatc cgtccgccga
     7801 cgccctggcg cttcgccttc tcgccgtttg ccggatccat tgcggccgtc gggctcgggt
     7861 tgtggctggg cgggatgctg atggagctcg aggaaccggt gtcgccgcct gcggcgaaca
     7921 cgacgctggc cgccatgagc gtgttcggtt ccgtcccacc gggaaatctc tgtccgcggg
     7981 ccggggcctg cggcgcaagc gggaacgcgc gatgaaaacc accaagcctg gaatggcgct
     8041 gctgctgtcg gtcgcgctca acgtcggcgt gctcggtgcc gtgctgctgg atcggctcgg
     8101 gccgggggcg tcctcgacat cggcgccgcc cctccatcgc atcctggaac tcgacgaggc
     8161 gcagcgcgcg cgctgggacg cggtcgaagc gcccttcctg cagcagttcc acgctgccag
     8221 cggccagttg gaggcgcatc ggcgggcact cgtcgaagcc ctctttgccg aggcgatcga
     8281 tccgctgcgc atcgagaccg cgcgcgcggc gatcgccgag ctgcagcagg accagcagcg
     8341 cctgatcatc gagcagctga tcgccgagcg cgagattctc gacgcgcgcc agcgccagca
     8401 gctgctcggc ctgctccttg cccagccgcc ggcacgcacc acggtcgagg acctgcacgc
     8461 gcgcccgacg cgctagcgca tctcgaacgc ctcctggtgg aagctcgcgc cgcccatgcc
     8521 gaggccgcgc aagcccttgc gcaggctggt ggcgaggccg cgcggcccgc agaaccagat
     8581 ctccggcgcc gcgcccgcgc gcgggcggtc gcgcagcgtc gccgcggtga ggcgctcgtt
     8641 gcgggcgccg ctgatcacgt gcaggcgcac cgcgggcagg tttgcgcaca aggcctggag
     8701 gcgggcgacg aaggggtcgg tcgcatgctc gcgcacgctg tagtagaaat cgacgtcgga
     8761 cgcctgctcc ggcctcgcct gcagcgcctc gagccaggcg aggaaggggg tcacgccgat
     8821 gccgccggcg atccagatct gctcccgccc gtccagcgac tgcggcagat cgaagcatcc
     8881 ataggggcct tcgacctgca tgggccgacc cggctgcagg cgctgcgcga ggccgcgggt
     8941 gtagtcaccc agggccttga tctggaagcg cacccgacgg tcgccgcggt cggcgccggc
     9001 gatcgtgaag ggatgcgcgc cctccagacg atcgaaggtc gcgaaggcga actgccccgg
     9061 ccggtgtccg cgccagccct cgtcgagccg gcagaccacc tcggtgacgc ccgcgccggc
     9121 gtcgcggacc gactccacca cgccgccgac gcggcggtcc tggccgatgc gcccgcccag
     9181 cacccgcacg ctggcgacgc tgccggcggc gatcaggccc gccagcagca cgccgaccgg
     9241 ttgcgtccag tagtcgagcg gcgcgaggaa ggcggcatgg aaggcgagcg ccaggtacag
     9301 gaccggcatc cccttgtgaa cgtaacgcca gatccggaac gggaagcgct tccacagcgt
     9361 cagcaccagc atcgccagca gcgcgtagat cgcgaactcg cccagctcct cggcgacgtc
     9421 gcgcatggct tcgagcaggc cgccgccgtg atccttgggc aggcgcccct cgcgccccca
     9481 cagcgtcttg atgaggtcgt cggagagctc gaccagccag tgcagcaccg cgaagccgac
     9541 cgcgaggatg ccggcccatt tgtgcacacg gtagatgcgg tccatgccgc cgaaaaaacg
     9601 ctccagccgg gccggacggg tggcgagcac catcgcaagc gacatcagcg cgaaggaccc
     9661 caggccggtg agcgtcagcg cgtgctcgcg caccgcccag ggattgcccg gcgcgctggc
     9721 gacggcgccc gtcgccgaca gcatttccac gccccaggcc aggccgacca gggcgatgaa
     9781 ggcggacaga aagcgtttca tgatccggtc cctccttcag tcgtcctcga agtagccctt
     9841 cgcggcgtcg atgtggcacg cggtgcagtt ggccttgctg ccgacgtcct tgcgggtcca
     9901 ctcggagcgc gggacctcgt cgtgctcgcg cacccacttc ggcagttcgg tgatgcgcag
     9961 gggggcatcg ccggggcgca ggccgcgcag cagcttgccc ggctgcccgc gcgcgtcgcc
    10021 ggcgtgatcg acgaggtagc gggtgatcgc ggcggtggtg tcggcatcga cgctggcgtc
    10081 gtcgccgaaa tgattctcca gttcgcccat catgcgtgtc cacgacgccg cgggcagcat
    10141 cgacgcaggg aaggcgatgt ggcagctcgc gcactcctgc acggtgagcg ggtcggtgac
    10201 cggcggatag aagtcgtcgt cgccggcctg ggcgcggccg agcagcatga gggtcacggc
    10261 gatcgcggcg gcaccgaggg tgagggggcg aagggcgggg gtcttcatgg cgggtctccg
    10321 gggtggcgct tactgggtgc tcatccaggc gagccagtcg cccttctcgc gtgcgctgca
    10381 ctcgcggccg agcacctcgt tgcagttgcg cttgaaccat ttttcgacct tcgccgggtc
    10441 gccgtagcgt gtggccgaca ccgacaccgc catcggctcg atggccttgc cggtgcgggt
    10501 ctggccggct gagcgcgcgt tctcgccatg gcaggaggtg caggcgggcg tgtcgggctt
    10561 gccgaggccg tggcgggtgc ggtgcagggc ttcgccgcgt gcggcggaaa aaccggcgaa
    10621 ggcggggtcg gtggcgcgcg cctcggcggc gtagcgctcc aggagggcgg cgctcggggc
    10681 ggcgagcgcc ccggcactgg cggcgaggcc gcacagcagc gcaaacgcgc ggaattggct
    10741 gttcatggtt cgttccttga tccgggctga ggactcgacc ggacgggcat cgcggatgcc
    10801 agtcgtccgg aatcgcgagc ccagtgtgca agcgcgccac tgaatcgaac ctgaacggag
    10861 aaagcgcgtg cgccaaggag cggcgggggc gggcgccgat gcgcccgcgc gctgcccgcg
    10921 ctcagcgcat gtcgaagagt tcctggtgga agtcggccgc cggcaggccc cgggcgacga
    10981 agccctcgcg cagggactgg ccgaagccgc ccggtccgca gaaccagacg tcggcttcgg
    11041 cccatcccgg cacggtgtcg cagatgcgcg ccacgtcgag gcggccgtcg cgcgcgctca
    11101 ccagcacgtg cagcctcacc cccgccgcct gcgcatcgcg ctgcaggcgg gcgatgaagc
    11161 cctggtccgg ttcggccgtg ctgtagaaca gatcgacctc gcgcccgtcg ggctccaccg
    11221 cgagcgcctt catgcgggcg atgaaggggg tgatgccgat gccgccgccg acccagatct
    11281 ggcggcgctt gccgctggcg ctgccgctgg cgaaatcgaa gcggccatag gggccttcga
    11341 cggtcacgag gtcgccgacg cggagcagtt cgggcaggcg cgcggtgtag tcgccgatgc
    11401 ccttgatcag gaagaacagg cggccgtcct ccttccagcc cgaggagatg gtgaagggat
    11461 gcgcgccttc gtccctggag aaggtcacga aggcgaactg gccagcctcg tgccccggcc
    11521 agcggtcctt gaagcggatc gccaccttga gcacgcggtt gtcggggaag ggcaccagct
    11581 cctcgatctc gccgaccgcc ttgcgccggt ggccgacacg gccggacagg gacaccacgg
    11641 cggcgacgct gccgccggcc atcagcacgg cgagcacgat gcccatcggc tgcgtccagt
    11701 aggcgaacgg cgtcagcagc accgagtgga agaccagcgc caggtaggcg agggccagca
    11761 ggcggtgggt cttgaagaac agccggtacg gaaagcgctt ggcgagcgcg atcacgatca
    11821 gcaccaccgc cgcgtagaag gcccactcgc cgaccgattc cgccaggccg cgctgtgcgc
    11881 ggaagaagct ctcgaccggg tcggtgagcg gcggcttcgg cccccgctcg ggcttgacca
    11941 gccagcccca gcccaccgcc cacttggtgc cctgcgccca cagccagtgg atgaccgaga
    12001 acaccaggcc ggtgatgccc agccacttgt gcaggcgata ggtcttgtcc agcccgtcga
    12061 gcagcggctc gacccgcacc gggcgcagcg ccaggatcat ccccacgctc atgacgccga
    12121 tgccgatcac gccggtgtag ttgatcgcca ccgtgcgcag ggcgaagtat tcgtagccgg
    12181 caggcagcag cggatcggcc atccaccaca gcaggctgag cagcagcagg agtcccgcgt
    12241 acgcaaatcg gatgttcttc atcgtgctct ccctcggatc gcatttcggc gcccgcggca
    12301 aggcgcggcc cgcacgccgc gtggagcgcc acctgcgtcg agtatccgcg aaaagccagc
    12361 ggcccgcctt gtcgccgcgc aaggccgccg cgcaccgaat ggcgacgccg ttctggggct
    12421 ggccgttcag gtccggttaa ggatcgcggc cccatgctgc gggttgtccc gaccacggag
    12481 cctttccaat gatgaccaag agcttgattt cgacgctgct gctggcgctt gccgccggga
    12541 tcgccaccca cgcccgcgcc gccggggacg aaccgacggc gctgtcgccg caggcctgcc
    12601 aggcgctgca ccaggagctc gacgcagtcg cggcccatgt gcgcgaggcg cgcgcgagtg
    12661 gcgccgcaca cgcggacgcg cccgatccct acccggcgct ggcgcaggca ctggaggccc
    12721 acctcgatgc cttgaaggcc ggcctgccga cgccggcgac accgcgcgcg caggctggcg
    12781 agttgttgag cgacatgcgt gacgccctgg tcctggtgcg cggcgccacc cacctcgaag
    12841 cccgcctgct cgcggtgcag cgcctcgagg acgatcgacg gctctacaat cgcgtcctgg
    12901 agaccctggg gtgctccgcc actcgaccca ccgcactttg aaccgatgac ctcgatcacg
    12961 ccgcgccagg ccctgctgct ctcgctcgcc gcagcgctga gcaccatcgc aatgaagacc
    13021 ggcgcctggt ggctgaccgg ctcgatcggc tatctgtcgg atgcgctcga atcggtggtc
    13081 aacctggcgg gcgcgggttt tgcgctgtgg atggtgtcct acgcgcagcg cccggcggac
    13141 gaggatcacc agtacggtca tggcaaggcc gaatacttct cggccgcgtt cgagggcggg
    13201 ctgatcttca tcgccgcgct gatcatcctg atcaccgccg gggaacgcct gctccatccg
    13261 caggcgatcg gcgacctcgg gctcggcacc ttgctgtccg tcggcgcgag tctgatcaac
    13321 tttctcgttg cgcgcgtgct gctgcaggtc ggtcgcgcgc accgctcgct cgcgctcgag
    13381 gccgacgcgc gccacctgat ggccgacgta tggaccaccg cgggcgtggt ctgcggtgtc
    13441 ggcctcgcgg gcgccaccgg ctggagctgg ctcgacccgg tggtcgccgg tgcggtcgcg
    13501 ctcaacatcc tgcgcgaggg ctgggagctg atgcggcgct cgatcgacgg cctcatggac
    13561 cgcgcgctgt ccgagaccga ggtggccgcg atcgaacgta ccctggccga ggcctcgccg
    13621 ccgggctgct tccacggcag gctcgccacg cgggccgcgg gtgcgctgca cttcgcccac
    13681 gtcgagctgt gcgttcccgg ggagtggacc gtcgccgagg cccacgcggt ggccgatgca
    13741 ctcgagggca tcgtgctcga gcgcaccggc acccgcctct cgacccacct cgagcccctc
    13801 ccgccgaacc tcccgcaggg cgcgggcgtg gacttgggcc gcgtgcccga cgaagggcca
    13861 tagacgaacg agaccccatc ccgccgatgc acctgctcat catcgaagac gaccgcacga
    13921 tcgccgagaa cctctacgag tacctcgagg cgcgcggcca ccagtgcgac tatgccggct
    13981 cgatcgcggc ggctgcggcc ttgctcgcgt gcaccgcgtt cgacgccctg gtgctcgacc
    14041 gcaacctgcc cgacggcgac gggctcgcgc tcgcacgccg gctgcgtgcc gagggacgtg
    14101 cgatgccgat cctggtgctg actgcgcgcg acgcgctcga agacaagctg ctcggcttcg
    14161 aggcgggcgg cgacgactac ctggtcaagc cgttcgcgct gcaggagctc gaggtccgcc
    14221 tgctggcact ggcgcggcgc agtgccgtgc ggccggccga cccggtgttg cgccacggcg
    14281 cgctggagtt cagggccgcg acccaggagc tgcgcctgca gggccgtctt ctcgccctgc
    14341 cgccgaagcc cttgcgcctg gtcgccgaac tgcttgccca gcccgagcgc gtgttctcgc
    14401 gccgcgagct cgagatcgcg gtatgggggc acgagcagga cagcagcgac aacctgcgca
    14461 gtgtgctgca caccctgcgg cgcaccctcg gcgacgacgc atccacccag gtggtgaatg
    14521 tccatggcct cggctacaag ctcgtcagcc gctgactgcg gcgcgcgccg cggcggccgg
    14581 cgttggctgc cggacggcat gcggatccgc atcgcggtgt cggtcacggc cgtgctggtc
    14641 gtgctcatcg tggcgcagtc gctcgccctg gtgcggctca acgaggagat ggaggaggag
    14701 ttcatcgacg gcacactcga ggagcagctg cactacagca tcgagcatag ccgccgcctc
    14761 ggtaccctga tcgggccgca gactccgaac atgacgctct accgcttcgc gccgggtgcg
    14821 ctgccaccgc aagggctgag ccccgcgctg gccggactcg cggtgggcaa tcacgaggaa
    14881 tggtccgccg ggcgcgagct gcacgttgca gtgcgagagg ccgatggcca gcgctacgtg
    14941 ctcgtgtacg acgaatccga gcatcgcaag cgcgagtcgg ccgtggtcgc cacggtcgtg
    15001 atcggcggcc tggtgctgag cgccttgtcc tttctcctcg tctacgccat cgccgcacgg
    15061 ctcacgcgcg ggctggagac gctggccgag cgcgtcgagg aggggggcga cggcacgccc
    15121 ttcgccgagg acggactcga ggccgagctc cgcgtggtgg cgcgtgcgct ggacagggcc
    15181 gaggcgcgcc aggccgccgt gctggcgcgc gagcgcgatt tcaacgccaa tctgtcgcac
    15241 gagctgcgca ccccgctcgc cggtatccgc agcgatgccg agatgctgct gaacgatccg
    15301 gtcctgtccg acaaggcccg ccgccgtgcc gagcgcatca tcgccaccac cgaccgcacc
    15361 accacgctcg cccaaagcct gctgctgctc gcccgcgagg cgcggccgca ggcgctcgaa
    15421 ccgatcgacc tgagcgcggc ggtgcgcgac gcctggacgc gcttgcaccc ggccggcgct
    15481 gcggccgcag cgcccagcat ccgtatcgac ccccaggccg agctcgtcgc cgaccccggc
    15541 ctgctggcgc tcgtgctgcg caacctgctg gagaacgccc tgcgtcatgg cgaggggcgc
    15601 gcgatcgagg tggtgctgga agggcgtcgc ctcgaggtgt gcgatcgtgg ccccggcttc
    15661 ggcgacgagg acccggcgcg ctgcttcgag cgcttccggc gcggcggggc caagccggga
    15721 cacgggctcg gcctggcgct ggtgcggcac atctgcaccg cctgcggctg ggaggcccgc
    15781 gccgccaacc ggcccgacgg cggcgcctgc ctgacgatcg acttcgggcc cgcgtcgaga
    15841 tagcgcggct ggcgtggttc cggctgcccg cgctcacact ttcctcacac ccgcatcacg
    15901 ctttcctcac gcccgcctgg gtagattgcg ccgatccttt ccggctccgc gcgatcatgt
    15961 tcaagttacg tctttcccac gtcccgtcct tcgctccctc ggccggcgca tcgacgcccg
    16021 tcgggcgtcg cgctctgggc gcttcggcgt tcgccgcagc ctggtccggc gcgcgcagct
    16081 ggcgccccga ggtgtcgggc gagggcttgc tggtcgggct cggcctctat ttcgcgctcg
    16141 cctgcaatac gcccttctgg cgtgcgctgc tcgcgagccg tggcggcgag ggcggcggcc
    16201 tcttctatgt gctcgcgctc ggcgtggcgc tcgccgcgct caacgtcgcg ctgctcgcgc
    16261 cgctgctcaa ccgctggacg accaagccgc tgctgggcgg cctgatcctc gtggccgcgg
    16321 tgtcgagcta ctacgccggc cacttcggcg tgtacttcga cccgagcatg ctgcgcaacg
    16381 tgctgcgcac cgacctcgcc gaggcgcgcg agctcctcac gcccggcttc ttcctgcagg
    16441 tcgccgcgct ggccctgcca cccctcgtct ttcttgcgcg agcgcgcgtg cgccggcgtc
    16501 cgctgcggcg cgcgctggcg atccgggccg ccgccgccgt gctcgcgctg ctcgtggccg
    16561 tcgcggcgct cggtagcgtg ttcaaggact tctccgggca gatgcgcaac cacaaggagc
    16621 tgcgctacct gatcaccccc gccgcgccgc tgtggtcgct cgcgcgcgtg ctgagccgcg
    16681 acgcgcaggc ggccaatcag ccgcgccggc cggtcggcgc cgatgcccgc ctgggagcga
    16741 gctgggcggc ggcgaagaag ccgaccctgt tcgtgatcgt ggtcggcgag accgcgcgcg
    16801 ccgccaactg gggcctggac cgtggggcgg ggcagtcgcc cgcccacgac accacgcccg
    16861 agctcgcccg ccgcgcggtc atcaacttcc cggacgtgac gagctgcggc accaacaccg
    16921 aggtgtcggt gccctgcatg ttctcgctgc aggggcggcg caactacgac gaggatgcca
    16981 tccgcggcag cgagtccttg ctcgatgtgc tgcgtcacgc cggcctgcgg gtggtgtgga
    17041 acgacaacca gtccggctgc aagggcgtgt gcgccggggt cgagagcctg cgccccgacc
    17101 ccgccgcctt gcccgcgctg tgcgacggcg agcgctgcct cgacgaggcc ttgctggaga
    17161 gcagtcgggc gctgctgcgt gatccgcaag gcaacctcgt gctggtcctg catcagcttg
    17221 gcaaccacgg tccggcctac ttccgccgct atccggaagc cttccgccgc ttcacgccga
    17281 cctgcgacga cgaggacctg tcgaagtgca cccgcgagca ggtggtcaac agctatgaca
    17341 acgcgctgag ctacaccgac cacgtgctcg cccgcggcat cgacctgctg aaggagctgg
    17401 agccgcgcta cgacgccgcg ctgctgtatg tctcggacca tggcgagtcg ctcggcgaga
    17461 acggcctcta cctgcacggg ctgccgtact cgatcgcgcc cgcggagcag acccgcgtgc
    17521 cgatgctgat gtggctgtcg tccggcttcg ccgcgcgcaa ccgggtcgat gcggcgtgcc
    17581 tgcgcgggca ggccgcccgg cccgccagcc atgacaacct cttccatacc gtcctgggcc
    17641 tgctcgacgt gcgcaccgcg gtccgcgacg acgcactcga cctgaccgcc ccctgccgga
    17701 gctgagcaca tgcacgccag cgaactcttc accccgctgc cggtgcccgc gctcgatgcg
    17761 tcgcggcgct ggctgctcgc gcagtccgca ctgctcttcc tcggtgcact gctgctctac
    17821 gggacattcg agcgaaccga tctggatcgc cgcctggcgc acgcgctgtt cgatgcggag
    17881 ctcggcctct ttcccctgag gcacagctgg ttcctcgagg cggtgatgca caaggcggcc
    17941 aagcaggcca cctacgtgct ggtggcggcc agcctgtacc tgtgctggca gggctggcgc
    18001 ggccggctgg cgtggctgcc cgcgcgcaat gcactgctcg cggcgatcgg catgctcgcg
    18061 attccgctcg cgacctcgac cgccaagctg ctgacggcgc gctactgccc ctgggacctc
    18121 atcgacttcg gcggctacgc accctatctg ggcctgttcg aggctgcgcc gccggggctc
    18181 aaggcggggc agtgcttccc ggccgggcac gcgtcgaccg gcttcctgtg gatcgtctgg
    18241 gcggtggcgc tgaaaccggc cgggccgcgt gcggcgcgcg ccgcgctcgt ggcgggcgtg
    18301 ctcgccggcg gcgtcctcgg tacggcgcgc atgctgcaag gtgcgcactt cctgtcgcac
    18361 acgctgtgga ctctgtggtt ggcgtgggcc gtgagcgtga gcctggcggt gctcgtgcgc
    18421 gccgacctcg gtctcgcggg gcggcgcggc gcatgacgcg tccgcgctgc ggcggaccgc
    18481 agcgctgctc cgtcagcgca ggtcgacgcg gccgccccgc gtgcgcttga tcccggtgat
    18541 catcgcgccg accaggttca cccgctccag ccggcccatc acgatggccg ccgcggcatg
    18601 gatcccggcg agtgcgatca gggtggcggc gagcgcctcg tggatctcct ccagccattc
    18661 ctcgccccag aacgcgtccg tggtctgcag gaagccggtg aggccgagtg cgaggaccag
    18721 cgccatcagc gcgagcatca tcagcgcgcc caggggattg tggccgggct ggaagtcgcg
    18781 cctgccggtg cgcatcgccg cgaggtgggc gcgcagccgc acgggggtcg ggaagaagtt
    18841 ggcgaagcgc gcgtattcgc tgccgacgaa gccccagacc aggcgggcga cgacgagacc
    18901 cgaggcggtg tagccgacga actggtgcag cgtctcgccg tcatcgacga cgaagaagtt
    18961 caggatgacg cagctcacca ggctccagtg gaagacgcgc acgaacgggt cccagacccg
    19021 cagggcaagt tcggaatcgg gcgggcgcat ggcagaggct ccttgtcggg tggaggaggg
    19081 cggcgcgcgg ccgcgcgccg ccccgatggc aggtcacttg cgttcgagtt cggcgccggt
    19141 ctcgggattg aagtagatct ccaccttctt tccgtccttg tcgaagccgt agatctcgta
    19201 gcagtcgttc ttggtcacct tgaagttctt gatcttgtag ccctgggctt cgaccttggc
    19261 cttgaagtcg gcctccttca tccacttctc cttgggggcg gtgcaggtcg ggccggcgag
    19321 ggccgaggtg gaaacgagcg cagcggcggc gagggcgaga atgcggttca tgtgtgttct
    19381 cctgtttgtc gatcgagcgc gccccgatcc ggaacgcgac cgcaatttaa gcttccgtac
    19441 atgaagggaa gctgaacagg caggggcggt gatccggtgc gcgacgcccg cggttggttc
    19501 ctccttaagc tgcacttaag ccagcatggt caggatccgg tgcagttctc atcaacggac
    19561 cccgacacac atggacctcc catggctcga ccacccagcc cgcccagcgc cgggcgcaca
    19621 gcccgcggcg cgcagtcttc gcctgctcga ggtcgatgac gcccgccgcc ccgccgtgga
    19681 gcacttcgtg cgcgaacgct tcgcggcgca ttaccaggcg cgtgtacgcc acttcatgcc
    19741 gcggctgttc gcactcgaag ccgccgacgg cagcctgcac ggggcggtcg gctgccgtgg
    19801 cgcggcagac caggggctct tcctcgaacg ctatctcgac gagccggtcg agcgcgtcat
    19861 cacgcggcgg gcgggagagc gggtggcacg cgacgggatc gtagaggtcg gcaacctcgc
    19921 cgcccacggg gccggcaccg cacgcctgct gatcatcgcg ctcacccgca tcctggccgc
    19981 cgaaggcctg cgctgggtgt gcttcaccgg aacgccggcg ctgatcaaca gcttccgccg
    20041 cctcggcctc gatccgctca cgctcggccc tgccgatccg cgccgcatgg gtgccgagct
    20101 gccggagtgg ggcagctact acgacgccgg cccgctggtg atggcgggcg agatccgggg
    20161 cggggatcgc accctcgcgg gcgccggccg cgagctccaa tccgggcgtg gagggcagct
    20221 ccgtgccgga tgcgtctgag tccttcctgg ccggcctcgc ccgccatggc ggccggcccg
    20281 cgctgcgtgg cgagagtggg gagattgcct tcgaccgcct gcccgaggcc gtcgccgcgt
    20341 gcaggggtgt gctcgagtcg gtggctgcgc gccgtgtcgc gctcgcgctc gacaacggcc
    20401 tacagtgggc gctctgggat ctcgcgctgc ttgccgacgg gcgggtgtgc gtgccgctgc
    20461 cgggcttctt ctcgccggcg cagcaggctc acgtgctcga cagtgccggc gtcgatacgc
    20521 tgatcgtcga cccagtggcc gccagcgcga gcgcggcggt gttcccgggc ttcgtgccgg
    20581 tcgcgcccgg gatcctccgg cgcgtgccgg cgcaggttcc ggcgctgccc acgggcacgg
    20641 tgaagatcac ctacacctcc ggcaccaccg gccagcccaa gggggtttgc ctgagcgcgg
    20701 cggcgcagct cgcggtggcg cgcagcgtgg cgcggatcgg cgaggctggc gcggtcgagc
    20761 gtcatctggg cgtgctgccg ctggcgacgc tgctcgagaa catcgccggc ttgtacgccg
    20821 gcctgctcgc tggcgcctgc gtcgagttgc tgccgatgcg cacgatcggc ttcagcgggg
    20881 gcgggggctt cgatccggcg cgcttcctgc agaccctgca tgcccgtcga ccgcacagtc
    20941 tgatcctgtt gccgcagatg ctgctcgcct tggtcggggc cgccgagcag ggccacgcgc
    21001 cgcccgccgg gctgcgcttc gtcgcggtcg gtggcgggca ggtgtcggcg cggctgctgc
    21061 agcgcgccga agcgctcggt ctgcctgtgt acgagggcta cggcctgtcc gagtgcgcct
    21121 cggtggtgtg cctcaacacc ccggctgcgc ggcgcgtcgg cacggtgggg cggcccttgc
    21181 cccatgccgc gctgcgcatc gccgacgatg gcgaggtgca ggtgcgcggc gcgcacatgc
    21241 tgggctacct gggcgaggcg ccgctggcag accagctggt ggcaggcgag tggctgggca
    21301 ccggcgacct cggccatttc gacgatggtt tcctcgttct gcacgggcgc aggaagcacc
    21361 agttcatcac cgcctacggg cgcaacgtga atcccgagtg ggtcgaggcc gaactcgtcc
    21421 agcagggccc gatcgcccag gcctgggtgc atggcgaggc gctcgccgag aacctcgccg
    21481 tgctcgtgcc gcgccgcgcc gactgcagcg acgccgagct cgacgcggcg gtggccgccg
    21541 ccaacgcggg tttgcccgac tacgcgcgcg ccgggcgctg gctgcgcgcc gacgcgccct
    21601 tcaccccggc aaacggcctc ctcaccgcca acggccgcct gcgccgcgcc gcgctcgcgg
    21661 cgcactacct gccacgcagc ggcgccgacg gcgccgcctc ctgcgcgacc ttcacctcct
    21721 tcagcgacga gatcgacaca tgagcttctt ccagcaactc caggaccaga ccgcggccga
    21781 gcgcgagacg ctgttcgccg caccgatcat ccagcaggcg ctcgccggca aggtcgcgct
    21841 gccgagctac gtcgccttcc tcaccgaggc gtatcaccac gtcagccaca ccgtgccgct
    21901 gctgatggcc tgcggtgcac gcctgccggc gcggctcgaa tggctgcgcg gcgcgatcgc
    21961 cgaatacatc gacgaggagt acggccacca ggagtggatc ctcaacgacc tcgccgcctg
    22021 cggtgccgac cccgaggcgg tgcggcgcag ccagccggcg ctgcccaccg agctgatgct
    22081 ggcctacgtc tacgaccgca tcgcacgcca caatccggtg agcttcttcg gcatggtcaa
    22141 tgtgctcgaa ggcaccagca ttgcgctcgc cacccgcgcg gcggggacga tccgcagcag
    22201 cctgggcttg cccccagagg ccttcagcta cctgaactcg cacggcagcc tcgacctcga
    22261 gcacatggat ttcttcgcga ccctgatgaa ccggctcgac gaggacgacg accgcgccgc
    22321 ggtggtgcat accgcgcgcg tcgtctatcg cctgtacggc gacattttcc gcagcctgcc
    22381 gctggcggcc tgaggagcgg cgcgatggag ctgaacggaa cccgcgtgct ggtcacgggc
    22441 gcgagcgggg gcattggcca ggcgctggtc gagcagttgt gcgcagcggg cgcacaggtg
    22501 ctggcggtcg gtcgcgacgc ggatcggctc gcggacgcac aggagcgttt tccgcaggcg
    22561 ttgcgccggg tcgtcgccga tctcgccacg atcgccggcg tcgatgcggt ggccgctgcg
    22621 gcgcgggatc tcgggggcgt cgactgcgtg gtccaggccg ccggcctcaa ccgcttcggc
    22681 ctcgtcgagc agcaggacga cgacgccatc gcgacccagg tcgcgctcaa cctggtggcg
    22741 ccgatgctgc tcacgcggcg cctcctgccc ctgctgcgcg agcggccgcg cgcgctgctg
    22801 ctgttcgtgg gctcgacctt cggcgcgatc ggctatcccg gcttcgcacc ctacagcgcg
    22861 accaagttcg gcctgcgcgg cttcgccgag gcgctgcgcc gcgagctcgc cgacggcccg
    22921 gtgcgcgtgc tctacctggc gccgcgcgcc acgcgcaccg cgatgaacgc gagcgcggtg
    22981 gtcgagatga accgcgcgct cggcgtggcg atggacccgc cgcagcaggt cgcggccgcg
    23041 atcgtgcagg cgctgcgcga cgagtcgccc gagcgccatc tcggctggcc ggagaagttc
    23101 ttcgcccgcc tcaaccggct gctgccgcgc gtggtcgatc gcgccctgcg cgggcagctg
    23161 gccaccatcc ggcgtttcgc ccattccgaa tcggaggaaa tccgctcatg aagatgcccg
    23221 cccaagaaac gttcgccagc gggacgctcc ggctcgcccg ccgcgtggcg ctcccgctcg
    23281 ccttcccgct cctctccctg gtggccgcgc cggccgcctt cgcgctcgcc gacgccggca
    23341 agctcgaact gcaggcagtg caggggcgct gggccgaaat ccactacggg ctgcccgaga
    23401 agcagcgcga agcggccttc gccgagctcg ccgagcgcgc cgcccgggcg gttgccgcgg
    23461 agccggaggc ggccgagctg cgcatctggc acggcatcgt cctgagcacc ttggcggggg
    23521 cgaagggcgg cctcggtgcg ctcgggctcg tcaaggaggc caaggccgag ctcgagaccg
    23581 ccctgcggct cgacccgcag gcgctcgacg gctcggccta caccagcctc ggcagcctgt
    23641 actaccaggt gccggggtgg ccggtgggct tcggcgacga cgcgcaggcc gaaaagctgc
    23701 tgaagcaggc cttggcgatc aaccccggcg ggatcgatcc caattacttt tacggcgact
    23761 tcctggcgcg gcagaagcgc tacgccgagg cgcgcacggc gctggagagg gcgctcgccg
    23821 cccccgatcg ccccggacgt gcgagcgccg acgccggtcg gcgcgcggaa gcgcggcgcc
    23881 tgctcgagca ggtggccgcg aagctggcgc aaggcgcgca gtaggcgttc aatatgcgga
    23941 tgaatgcagt gcccatgatt gccgggatca caggggagga aggggatgcg catcctgctc
    24001 gtcgaggatg atgccgcgct cggcgagggc atccgcgtcg cgctcaagcc cgaggcctat
    24061 acggtcgact gggtgcagga cggtgcgagc gcgctgcatg cgctcaccca cgaggacttc
    24121 gcgctcgccg tgctcgacct cggcctgccg cgcatggatg gcctgcaggt gctgcagcgc
    24181 ctgcgcgccg ccgccaaccc ggtgccggtg ctggtgctca ccgcgcgcga cgccaccagc
    24241 gaccgcatcg cgggactgga cgcgggggcg gacgactacc tcgtcaagcc cttcgacgtc
    24301 gacgagctca aggcccgcat ccgcgcgctg ctgcgacgca gcgccaatcg tgcgcagccg
    24361 gtgctgcacc acggcgaggt cgtcctcgat ccggtgaacc agcaggtcag ctaccgcggc
    24421 cagccggtgg tcctgcagcg caaggagttc ctgctgctgc acgagctgct cacccagccc
    24481 ggccgcgtgc tgacccgcga tcggctgcag caggccttgt acggctgggg cgaggaggcc
    24541 gagagcaata cgctcgaggt gcatatccac catctgcggc gcaagctgtt tcccgagctg
    24601 atccgcaccg tgcgcggcgt gggctatctg gcggacaagg cctgatgggc tcgatccgcc
    24661 gtcgcacctt gctgctcgtg ctcggcctgc tcggcgtggc gctgggtgcg atctcctacc
    24721 tcggttatcg cgatgcccgc cacgaggtgc gcgaactctt cgatgcccgt ctcgcccagc
    24781 aggcgcgcct gctcgcgggc atgatcccgg gcgggatggc accggatgca cgtgcggcgc
    24841 tgcaggacgc gctcgatgcg ggggggctcg gtgcggccgt gggggagggg acggacgcgg
    24901 aacgcgagga cggtgacgag gcgctgccgc tgggccacga gtacgagggc aagctcggct
    24961 tcgtggtggt cgacgaccag gggctggcct tgctgcagtc cgccgcggcg ccgatgggcg
    25021 cgctcgacct gctgctcggc gcacgcacca ggggcgccga gcaacagggg ctcggcgagg
    25081 tgggagagga tcttgcgggc tatcacacgg tgagtctgca cgatggcgcc tggcggctct
    25141 tcctgctccg ggacgcgcgc gaccgccagt ggatcctggt cggcgagcgc gaggacgtgc
    25201 ggggagaact cctgggcagg atcaccttgc gcagcgtgct gcccgatctc gtcgggttgc
    25261 cgttggtcgc cgtgctggtg tggctggcga tcggctgggg cctgcgcccg ctcgcgcgca
    25321 tcgtcgaatc cttgcaggcg cgcgggccgg acgacctctc cgccctcgcg ctgcaggacg
    25381 tgccgcagga gctcgagccg atggtggccg cgctcgaccg cctgctgcat caggtcaacg
    25441 agctgctcga acgcgagcgc cgcttcctcg cctatgccgc gcacgagctg cgcacgccgc
    25501 tggccgtgct gcgcatccat gcccagaatg cgctgcaggc gcctgatccg gccgatcgcg
    25561 aggaggcgct ccggctgctg ggctcgggca tcgagcgtgc cacccgggtg gtggcgcagt
    25621 tgctgacgct ggcccgcctc gaacccgacg cgagccggcc caagaggctg ccgatcgagc
    25681 tgctcgcgct tgtccgcgag cagctcgccg agctgacccc gctcgccgac gaacatggtc
    25741 aggacctcgc cctcgaggcg gacgaggggg ccgacttcca cctgctcggc gatgccggca
    25801 gcctgggcat cctgatgcag aatctggtgg gcaacgcggt gcggcacacg ccgcccgacg
    25861 gctgcatccg cgtgctgctc gaggccacgc ccgcagccat cgtgctgcgg gtgcaggaca
    25921 gcggccatgg cgtgccgccg gagctgcgcg agaaggtgtt cgagcgcttc ttccgcgccg
    25981 gtggcgggca gggggcgggt ctcgggctgg cgatcgtcgc gcgcatcgtc gaactgcatg
    26041 gcggcacgat cgcgctcgac ggctgtgcgc tcggcgggct ggaggtgcgg gtggtgctgc
    26101 cgcgggatgc cgccgcgccg cgccgggtgc agggcgatga ggggaaggtg ccgccgtgct
    26161 ccgcgtccgg cggagcggcc cgcccttaag cgtcgcttaa gacggcgtcc ttaccgtgaa
    26221 cctgcgctcc gcgtgggagc gctcgtcggg tgcaagtcgg ccctccggcc gttgccgagc
    26281 gcccggcgtg aacctgcgag cggggacggg aagatgccat cgaatgaaga tttccatgcc
    26341 ggcgagcgcg ccctgcagca gcgctggggc accgcatcgc tgtgggatgc ggcgcgccgg
    26401 gagcggctgc tgtgggatca cctcccggac tgggtgcagc cccggctcgc ggcggccccg
    26461 ttcttcttcc tcgccaccag cgacgccacg gggcgttgcg actgttcctt caaggggggc
    26521 ggccccggcc tggtgcgcat cctcgatgcg cgccgcttgg cgttcccgga tttcgacggc
    26581 aacggcgcgt tcatgagcct gggcaacctg cggcagaatc cgcaggtcgg ctgcctgttc
    26641 atcgacttcg gcgacggcgc ccgcctgcgc gtcaatggcc gggcgacgct gctcgagggc
    26701 gaggcggcgg ccaggctctt ccccgggcag gaccgcgccg tggtggtgga cgtcgagcag
    26761 gtcgtgccca actgcgcccg ccacgtcccc acactgcacc cccctactgc cgacgcgcgg
    26821 ggcgctgcat gaagattccc taccgcatcg ccgccaacgt cgagtggacc agcaagtgca
    26881 acgccgcgtg cgcgatgtgt ccgcgcgagg ccatcgaccg cccgcagtcg atgagcatgg
    26941 cgacctggcg gcaggtgctc gatcgcctgg cgccggacga gatcttccgg gtcgtgctgg
    27001 ccggttacgg tgagccgacc acgcatcccc gtttcttcga gttcatcgac gagctgcgcc
    27061 cgcacccggt gcgcttcgac atggtcagca atggtcacct gctcgacgtc gagcgcctgc
    27121 gccacctcga cggtgcgatc gacctgctga tggtgtcgtt ctccagcatc gacccggacg
    27181 tctatgcccg cgttcacgtc aatctcgacc agcgccgcgt gatggacaac ctgcaggcgg
    27241 cgagccgcgt cctgcgccgc acccggcttg cgatcagcct caccccgatg cccgactgcc
    27301 tgcccgcgct gggcgacacc atcgcgtggt tgcgcgcgca tgggatcgag cggctgacca
    27361 tgtcgcccac gctctacaac cgcggcggca agctcgaggc ccatcgcctg gcgagcgcgg
    27421 agctgcggcg gatgatccgc cagcacgggc tgcgctcgca ggaactcgac ttcgtgccga
    27481 gcgcggccga gatcttccgc cagtggcggc gcaaccgctt cggctgcatt ccgcgcaatg
    27541 tcgacctgtt cgtcgccgcg agcggtgact atctctactg ctacaacgat gtcgcgcacc
    27601 gccacccgat cggccatgtc gccgccgatc cggtggctgc ggtgttgcgg cgccgcgagg
    27661 gcatggcggc gctcccgacc ttgtgcgagg gctgcaacat gcgccatcgc tatggtcccc
    27721 gcgagctgct gcgggcaggg gtcgggtttg cccgggcgcg cctgcgggag ggatgaaggt
    27781 ctggcgggcg gccgtcgtgg cagtggtgtg gcggctgacg ggacggcgat acactgcggt
    27841 acacacgccc cccgcgggaa gactacattt gacgcggacg agcctgagag ctgccatgcc
    27901 ctccgggagg gccggcgagc aatgtccaac gtcgtaaagg agaagaccat gaagacgcgt
    27961 acgctgatgg caacggcaat gggtgtcctg tgcgcagggc tgaccttcgc ccagcaaccc
    28021 gtcgcggcgc cgggggagga ggcgcccgta ttcggcgccc agatcatgac cgagcaggag
    28081 cgcgtggaac accgcgagcg catgcgtgct gcgggtacgc ccgaggagcg cgagcagctg
    28141 cgactcgagc atcacaagca aatggtcgag cgtgcacgcg agcgcggcgt caccctcccc
    28201 gacgagccgc cgatgcgcgg cggcatgggg gcgggcggtg gcatgggggc gggagccggg
    28261 atgggggcgg gcggtggcat ggggccgggc ggaggacggg gacccggagc gggtatgggg
    28321 ctcggagggg ggatgggtcc gggaggacgc atgggatccg gagcgggnnn nnnnnnnnnn
    28381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28441 nnnnnnnnnn nnnnnnnnnn nnnnnnnctg gcggcggcgc cgcgcccgga ggagacgttc
    28501 ccgcgcgttg aggcgcggtc tgcgcccaag tcctcgagct gtcgccctgc aggccggggc
    28561 acgccaggct tgcctccgag gacgcgatcg tgcgcgatcc tgtcgaaacc gccctggccg
    28621 tcggcttcga gccgggcgcg gggggcggcc gatcgcgact tgcgtcgctc ccacgaaaat
    28681 cgcaggcgcc gcatgcacga gatgcgcgag ggctcccgtg taggcgcgat gcaagtcgcg
    28741 atcatcgcgg acggatctgc atggcgccga ctggcaacgc cacggccgcc tccgcgccga
    28801 gccaccgctt ggcttgcgcg cttgcggagg cgtggctcgg acagaacgag tagagttcag
    28861 tctctcgtgt acataagcat tcaacgatga agaagaatct ctggctgctc gcgatcgcgc
    28921 agggcctgtt cctgaccaac aacgtggtgt tcatcgccat caacggcctg gtgggcttca
    28981 gcctggcgcc ggtgggctgg atggcgacgc tgccggtgat gggctacgtg gtgggcgggg
    29041 cgttgtcgac cgggctggtg gcgcgcagcc agcggcgctg ggggcggcag gtctcctttc
    29101 agctcggcct gctggtggcc ttcctgaccg cgctgctgtg cgccttcgcg ctgtcgctcg
    29161 gcagcttctg gctgctcgtg gcgggcacgg tggtcgccgg ctactacagc gccaacggcc
    29221 agctctaccg tttcgccgcc ggcgagctgg cgccgtcgtc cttccgcgag aaggcggtgt
    29281 cgctggtgct ggcaggcggc ctcatcggtg cggtgatcgg tcccaacctc gccaaccaca
    29341 cgcgcgaggc gatggccgtg cccttcctcg gctcctatct cgcgctcgcg gtggtggcgc
    29401 tggtgtcgat ggcggtgatg gccggaatcc ggtttccgcc gcagccggtg aagaaggcgg
    29461 gtgccgaggg cggacggccg ctggccgaga tcctgcgcca gccggtcttc atcgtttccg
    29521 tgctggccgc cgcgatgagc tacggcgtga tgaacctgct gatggcggcc acgccgctgg
    29581 cgatggacgt gtgcggcatg cccttctcgg acgcggcgct ggtgctggag tggcacgtga
    29641 tcggcatgtt cgcgcccggc ttcttcaccg gccacctgat caagcgcttc ggcgtgctgc
    29701 gcatcatggg cgtgggcgtg gtgctgaact tcgcctgcgt ggcggtggcg ctctcgggcc
    29761 aggacctgca ccagttcctg ctcgcgctct tcctgctcgg cgtgggctgg aacttcctgt
    29821 tcaccggcgc gaccacgctg tcgatggaag cctaccggcc ggaggagaag gacaaggcgc
    29881 aggcggcgat caatttcgtg gtgttcgcgg tgatggcgtt tacttctttc gcctcgggcg
    29941 cgctggtgac gacgcagggc tggaatctgc tcaacgtggg ctcgatcgcg ccgctcgcgc
    30001 tcaccgccgc ggcgctgggg tggcttgcgc tgctgcggcg gcgtgcggcg gacgggcagg
    30061 gcgcatgaac ttcctcgccc acgcctggct cgccggagag tcgcccgcgc tggtggtggg
    30121 cggggtcttc ggggactggg tcaaggggac actcccgggc gcgctgcccg ccgacctcgc
    30181 acgcggggtg gcgctgcacc gcgcgatcga cgccttcgcc gagacccacc cggccttccg
    30241 cgccagccgc gcccgcgtgt cggcggcgcg gcggcgctac gccggggtgc tggtcgacat
    30301 cttctgggac catctgctgg cgcgcgactg ggcggagcat ggggaagggg cgctggcgtc
    30361 ctattgcgcc ggggtgtacc ggcagctcgc ggcgcgcagg gcggacctgc ccgcgtcggc
    30421 ccaccttgcg ctggagcgga tggcgcgcga ggactggctg ggcagctacg ccgggctgga
    30481 gggcatcgcc gacgtgctcg cgcgcatggg ccggcgggcg cgccagccca acccgctcgc
    30541 gggcgcggag gtggagttcg tggccgaccc ggcgggcttc gcgcaggatt tcgccgactg
    30601 ggtgccggat gcccggcgct tcgtggcgga gtggcgggac gggcgcgcgc gcggagggcg
    30661 ccgggccggc ttgaacgacg cggcgtgagc gaagtgctgc gagcgaggtg gggcgaagcc
    30721 acgtcaccac gcgaactggg ccgggtggtg tcgcgccgcc tcaggtcagg tgcaccgggc
    30781 gcgcggcgaa ggcctccgcc agggtggcga actcgcggct gcggtcggcg ctgaggcgat
    30841 agaaaacccg gttgtcctgc gtgcgcccgc aatagctgcg gatgacgacg cgctcgatgc
    30901 cggggatgcc gcggttggct tcgacggtct tgatcgctcc cgtgccggtg tcgtacacgc
    30961 ccgggtgttc gcggaatccg aaggggtcgt cgatcttgtg cgcgttcatg gaagaggctc
    31021 ctcgaaccgg aatcgcaacg ggggaaagga tagcaccggc gggtaggagg cgcgtacggg
    31081 ccgtcagtga aaatcccggc tgcgcgcggc gaggctgccg agcaggggcg agtggtcgac
    31141 cacccggctc gccaccagat gcacgacccg gccctggcgg cggatctggc cgaacacgcc
    31201 catcaggcgc gccccgagca ggatgcggcg ctgctgttcg aagagctcgg ggcggacgat
    31261 cacgttggtg agcccggtct cgtcttccag ggtgaggaag agcgtgccct tcgcggtgcc
    31321 ggggcgctgg cggcaggtga ccaggccggc gccgcgggcg agcatgcggt cgggcgcggc
    31381 ggtgatgtcg gcggcggtga gaaagcgcag gcgggcgagc tgctcgcgca cgaaggcgag
    31441 cgggtggcga ccgagggtga agccgaggcg ggcgtagtcg gcgagcaggt cttcggcctc
    31501 ggcgggggcg tccagcgtgg cggcgacgtc gccgccaggg gcttcggcga gcaggccggg
    31561 caggggcgcg gcgccgctcg cctgccacag ggcctggcgg cgatggccgg cgaggctggc
    31621 gagggcaccg ccggcggcga gggtgcgcag ctcgccggtg tcgagccgcg cgcgcgcggc
    31681 gaggtcctgg gtgtcggcga aggcgtgttc gccacgcgcg gcggcgatgc gcgtggcggc
    31741 ctcgcggccg aggccgcgga tcatgcgcag gccgaggcgc accgcgggct gcgcgccgtc
    31801 ggcggcctcc agcatgcagt cccacgcgct ggcgccgacg tccgccgcgc gcacctccac
    31861 cccatggcgg cgggcgtcct ggatcagctg cgcgggcgcg tagaagccca tcggctggct
    31921 gttcagcagc gcggcgagga aggccgcggg cgcgaagcac ttgagccagg ccgaggcata
    31981 caccagcagc gcgaagctgg cggcgtggga ttcgggaaag ccgtagctgc cgaagccctc
    32041 gatctgctgg cacaggcgct gggcgaagtc ggggtcgtag ccgttgttcg ccagcccgtc
    32101 gagcagcttc tggcggtagc gctcgagctc gcccttgcgc cgccaggcac ccatcgcgcg
    32161 gcgcagctgg tcggcctcgc cgccggtaaa gtcggccgcg accaccgcga gctgcatcac
    32221 ctgctcctgg aagatcggca cgcccagcgt gcgcgccagc acaccgcgga tctcttcgcg
    32281 caggccgtcc aacggatcct cgccgcgcgc cgcacgctcg cgcgcctgca ggtagggatg
    32341 gaccatgccg ccctggatcg ggccggggcg cacgatcgcg acttccacca ccaggtcgta
    32401 gaagcgttgc ggcctcaggc gcggcagcat ggtcatctgc gcgcgcgact cgacctggaa
    32461 cacgccgacc gaatcggcgc gcgacagcat ttcgtagacc tcctttcgtt cgcgcgggat
    32521 ggtcgccagc gtcagcgcct cgccgcgcca tgccgacacc agggcgaggc cgcgacgcag
    32581 cgccgacagc atgccgagcg cgagcacgtc gatcttcatc aggccgaccg cgtcgaggtc
    32641 gtccttgtcc cactggatca cggtgcgatc ggacatggcg gcgttctcca ccggcaccag
    32701 ctcgtcgagc cggccgcgcg cgatgacgaa gccgccgacg tgctgggaga ggtggcgcgg
    32761 aaagccgatg agttcctcgg tgagtgcgac cagcctttgc gtgaccgggc tggcggggtc
    32821 gagcccggcc tccgcgaggc gctcgggcag gatgtggcgg ccgtcgaacc agtggtgctc
    32881 gcgggtgagg cgctcgatct gcgtctcgtc gaggccgagc gcgcggccga cgtcgcgcag
    32941 cgcgctgcgt gcgcggtagc ggatcacggt ggcggcgagc gcggcgcgtt cgcggccgta
    33001 cttgcggtag aggtactgga tgacctcctc gcggcgctcg tgctcgaagt cgacgtcgat
    33061 gtcgggcggc tcgtcgcgct ctcgcgagat gaagcgctcg accagcatga tgcccagctg
    33121 cgggtcgacc tcggtgatgc ccagcgccca gcacaccacc gagttggccg ccgagccgcg
    33181 gccctggcac aggatgccgc gctcgcgggc gaagcgcacg atgtcgtgca cggtgaggaa
    33241 gtagggctcg tagccgagct cggcgatgag cgcgagctcg tgctcgatct gcgcgcgcac
    33301 cgtgggcggg gcggggtctt cggccggcgg cggtgcggcc gcgatcgtgg caggcgcggc
    33361 tgcgcggcgc tccttgccgg ccttgccgta gcgccaggcc agcccgccct cgaccagccg
    33421 gcgcagccag cgcgtcgggg tctcgtccgc ggggacgagc tcggcggggt actcgtagcg
    33481 cagctcgtcg aggtcgaagc ggcagcgcgc cgccaccgcc acggtctcgg cgagcagctc
    33541 gggaggatgg cggcgggcga gggtcgcgcg ctcgtgcagg cgcagctcgg cattgggcgc
    33601 gagcgccagg ccggcctcgg ccacgctgca gtgcaggcgc agcgcgctca tcacgtcggc
    33661 gagcgggcgc cgcgcggcct cgtgcatgag cgcgccgccg gcggcgaccg cagggacgcc
    33721 ggtggcctgg gcgatgcgca ggatccagtg caggcgggcg ggatcgcgtc cgtccaggcg
    33781 cggggtgtgg gcgatccagg agcgtccggg gaagcgcttc gccagccagc gcgcttcgct
    33841 gcgcagggcg atggccgcgt cgccggcggc acccggcggc gggaattcct cgggtgggat
    33901 cagcagcgcc aggcagccgg gcagggcttc ggcggcagcc tcgtctgcgc gggtggcgtc
    33961 gagcatcgcg cgcgtgaggt ggtagcggcc cttgtcggcg ctccgcctgg cctgggtgag
    34021 caggcgcgag agccggccgt agccggcgcg atcggcggcg agcaggacga ggcagggccc
    34081 gtcggcgagg cgcagctcgc agccgacgat caggcgcggc cggccggctt cgggcagggc
    34141 ctgcagcgcg cggtgggcgc gcaccacgcc ggcgaaggag cattcgtcgg tgagggcgat
    34201 cgcgctgtag ccgaaggctg ccgcctgcgc gacgagctcc tcggggtgcg aggcgccgcg
    34261 ctggaagctg aagttgctca ggcagtggag ttcggcgtag gcgggaagca tgtgttgact
    34321 gtataaaaat acagttcatc aggcaagtcg ggcgagtgtc ctccacggcg attgcgggtc
    34381 caccgcaatc gcgcaatccg gacctgctgt taaactcgcg tcctttgcaa caccgtgctg
    34441 gccaagatgc cggcctggtg cctttttcga agaattcccg gccgcaaggc tgccaaggag
    34501 acctccatgt ccttccccct gtcccgcaag gtcctgttcg gcgcgctggc gctcgcgttc
    34561 gcgacggcga tgccggcgta cgcacagcag aagtcggtcg ccatcaccgc gatcgtcgag
    34621 catcccgcgc tcgattcggt gcgcgacggg gtcaaggagg cgctggccgc ggccggctac
    34681 gaggacggca agaacctgaa gtggcagtat cagagcgccc agggcaacac cggcaccgcg
    34741 gcgcagatcg cacgcaagtt cgtcggcgac aaggccgacg tgatcgtcgc gatcgccacc
    34801 ccctcggcgc aggccgtcgc ggccgcgacc aaggacatcc cgctggtgtt ttcggcggtg
    34861 accgatccgg tggtggccaa gctggtgccc tcgatgcagc cctcgggcac caacgtcacc
    34921 ggggtgtcgg atgccctcga gctcggcaag caggtcgagc tgatcaagcg cgtcgtgccc
    34981 gccgccaagc gcgtcggcat cgtctacaac ccgggcgagg ccaactcggt ggtcgtggtc
    35041 gagcagctgc gcgagctgct gcccaagcac ggcctgagcc tggtcgaagc cgcggcgccg
    35101 cgctcggtgg acgtcggctc ggcggcgcgc agcctgatcg gcaaggccga cgtgttctac
    35161 accagcaccg acaacaacgt cgtgtcggcc tacgaggccc tggtcaaggt gggcatggac
    35221 gccaagatcc cgctcgtggc cgccgacacc gacagcgtgg cgcgcggtgc ggtcgcggcc
    35281 tacggcatgg actacaaggc gctcggcgtg cagaccggcg agatcgtggt tcgcatcctc
    35341 aagggcgaga agcccggtgc gatcgcctcc gagaccagca acaagctgtc gctgcaggtg
    35401 aacccggctg cggcgcagaa gcagggcatc accctggcgg aagacctcgt caagtcggct
    35461 gccaaggtcg tccagtaagc gggtcgcccg catccccgcg cggccgtcga tgcccggccg
    35521 cgggggacgc gtcccggcgc gcgttccgct cgatcctcac caacggtaat cccccaatgt
    35581 cgctctacac cttgctcggg gcgctggaga tcggcctgat cttcagcctc gtcgccctcg
    35641 gcgtgtacat ctccttccgg ctgctgcgct ttcccgacct caccgtggat ggcagcttcc
    35701 cgctcggtgg cgccgtcgcc gcgaccatga tcgccgccgg ccacgacccc ttcaccgcga
    35761 ccatcgcggc gacgctcgcc ggcgcgatcg ccgggctgat caccggctgg ctcaacgtca
    35821 gcctcaagat catggacctg ctcgccagca tcctgatgat gatcgccctg tactcgatca
    35881 acctgcgcat catgggcaag cccaacgtgc cgctgatcac cgaacccacc gtgttcaacc
    35941 tgctgcagcc cgaggcgctc gccgactacg tgttccggcc cttgctgctc gtcttcgtgg
    36001 tgctggcggt caagctcgcg ctcgacggtt tcttcgccac ccagagcggc ctggcgatcc
    36061 gcgcgacggg ctcgaacgcg cgcatggcgc gtgcgcaggg cgtcaacacc ggcatggcga
    36121 tcctggccgg catggccctg tcgaacgcct tggtgggcct ggccggggcg ctgttcgcgc
    36181 agacccaggg cggcgccgac atctcgatgg gcgtgggcac gatcgtcatc ggtctcgcgg
    36241 cggtgatcgt gggcgagagc ctgctgccct cgcgcaagct ctacctggcg acgatcgcgg
    36301 tgatcgttgg cgccatcgtg tatcgcttct tcatcgcgct cgcgctcaat tccgacttca
    36361 tcggcctgca ggcgcaggac ctcaacctcg tcaccgcggt gctggtgacg gtcgcgctgg
    36421 tggtgccgcg cctgcgcgcg cactggtccg gaaaaagggt gagctgaaat atgttgaaag
    36481 caaccgacct tcgtctcacc ttcaaccctg gcacgccgat cgagacccag gccctgcgcg
    36541 gcatgtcgct cgagatcccg accgggcagt tcgtcaccgt catcggctcc aacggcgccg
    36601 gcaagtcgac cttcctgaac gccgtgtcgg gtgagcagcg cgtcgacagc ggcacgatcg
    36661 agatcgacgg catcgacatg acccgccagc cggtgtgggg gcgtgcgcgc cacgtcgcgc
    36721 gggtgttcca ggacccgctc gccggcacct gcgaggatct caccatcgag gaaaacatgg
    36781 cgctcgccaa gatgcgcggt gagcgccggg gtttttcgtt cgcggtcaag ccggcgctgc
    36841 gcgaggagtt ccgcgcgcag atcgccacgc tgggtctcgg cctggagaat cgcctctcgg
    36901 accgtatcgg cctgctctcc ggcggccagc gccaggcggt gagcctcttg atggcggcgc
    36961 tgcagccctc gcgcctgctg ctgctcgacg agcacaccgc cgcgctcgat ccgcgcaccg
    37021 cggccttcgt gctggaactg accgtgcgca tcgtcgccga gcacaagctc accacgatga
    37081 tggtcacgca ctcgatgcgc caggcgctcg acgtcggcca gcgcaccgtg atgctgcacc
    37141 agggccgcgt cgtgctcgac gtctccggca aggaacgcga gggcctcgac gtgcatcacc
    37201 tgctcgacat gttcgagaag gtgcgcggcg agaagatcga cgacgacgcg ctgctgctgg
    37261 gctgattccc cgggtcgagt ctcggggcca agagcctggg gcggggcctc ttccgcgcac
    37321 cggctggcca agcgacccgc ctgatccccg aattcaaacg aaacacgacc gaaagcaacg
    37381 atgacctccg cttcccgccc cgtccgtacc cccgtgcgta cccgcttcgc ccccagcccc
    37441 accggctacc tccacatcgg cggcgcgcgt accgcactgt tctcgtgggc cttcgcgcgc
    37501 cgccatggcg gcaccttcat cctgcgcatc gaggacaccg acgtcgcgcg ctccacgccc
    37561 gcggcggtgc aggccatcct cgacggcatg cagtggctgg gcctggaaca cgacgaaggc
    37621 ccgttctacc agatgcagcg catggaccgg tacaaggagg tcatcggcca gatgctggcg
    37681 gccggcaccg cctaccactg ctacctgtcg cccgaagaac tcgatcgcct ccgcgaggcg
    37741 cagcgcgagc ggggcgagaa gccgcgctac gacgggcgct ggcgtcccga ggcgggcaag
    37801 gtactgccgc agccgcccgc gggcgtgcag ccggtggtgc gcttccgcaa tccggccgaa
    37861 ggcgtggtga gctgggacga cctcgtcaag ggtcgcatcg agatcgccaa cgccgagatg
    37921 gacgacttca tcatcgcgcg cgccgacggc acgccgacct acaacttctg cgtggtggtg
    37981 gacgactggg acatgggcat cacccacgtc atccgcggtg acgatcatgt gaacaacacc
    38041 ccgcgccaga tcaacgtgct gcaggcgctc ggcgccgagg tgccgctcta tgcccacctg
    38101 tcgatgatcc tcggcgacga cggcaccaag ctctccaagc gccacggcgc ggtcagcgtg
    38161 atgcagtacg acgaggaggg ctatctgccg gaggcggtga tcaactacct ggcgcgcctg
    38221 ggctggagcc atggcgacga tgaaatcttc gatcgccggc agttcgtcga atggttcgac
    38281 ctcgaccaca tcacgccctc ggccgcgcag ttcaacaccg agaagctcaa ttggctcaac
    38341 gcgcagtaca tcaagaaggc ggacgacgcc ttcctggccg ccgaggtcgc cagccgcctc
    38401 gcgcgccgcg aggtgaaccc cgagacgggg ccgagcctgg aggcggtggt ggcgctgtac
    38461 aaggaccgcg tcgccaacct caacgaactg gcggatgccg cggagttgtt cgtcgccgac
    38521 gtgcatccgg caccggaggt ggtcggccag cacctgaccg acgtcgccaa ggccgccctc
    38581 gccagcctgc gtggccgcct ggccgcagcc gagtggaaca gggccgcgct gagccaggcg
    38641 atcaaggaca ccatggccga gcatggcctg aagatgccgc aggttgcgat tcccctgcgc
    38701 gtggcggtgc tcggcgtgcc caagaccccg gcgatcgacg ccgtgctgga ggtgctcggc
    38761 cgcgggcgcg tgctcgagcg cctcgaccgc cgcctctgaa cgcagccgca ggcgcgagtt
    38821 cgtgcgccgt atcgcgactt gcgtcgctcc tacacgcccc cttggaacga cgtgtgggag
    38881 cgacgcaagt cgcgatcatg tcggcgcggc tgcgacgggt gtcggcgggg actgcgggcg
    38941 cgaatcgcga cttgcgtcgc tcccacaggc gcgtccgcta gggcagcggg ctgcgtaggc
    39001 gtgaccccag cggtgtgcgt aggagcgacg ccagtcgcga ttgcagcggt gaaagcgggc
    39061 gcgggcgcgt gtccgtgcgc tgctcccgca aaaacaaacc gccgcgagtg cggcggtcgg
    39121 gatggcggtg cagggggctc aggcggcctt gcggatggcg cggctgcgcg gctcttcggc
    39181 ggcttcggac tggacgacct cgacgccgcc cagcttgcgc gcctgttcca gcatcgactg
    39241 catcgaggcc tggaagtctt cgaagtggcg gctgccgagt tcgagcagct cgttttgcgc
    39301 gcccgacacc acctcgttca cctgctgcag gtagttcccc agttgctcga gggcctgacc
    39361 ctgggtctcg cgctgcttcg cgaagccggc ctcgaggtcg ctgcccggtt gggcgatcgg
    39421 cgcggccgag gcggcctcgc acagcgcgcg cgcggcgctg aagttgagcg cgcacagctt
    39481 ctcggaagcg tcgagcgcga tcagggcata ggcctggaac tgctcgaagc cgttgcgggt
    39541 cgagtgggcg aactgctgga aggccggatg ggtgttcatg gcgaagtctc cgctaaagga
    39601 tgccgggtgg ttctctcagc cagcgatgtt aagcggcacc ccgttccggg catatacagc
    39661 gttcgcagtg tttggactac cggtttaccg gtagtgcgaa ggcgccgggc gcgctcagcc
    39721 cgcgccgccc ggggtcacgt tcgggctgat gccgctgtgg atcgcgtagc gggtgagttc
    39781 ggcaacgttg tgcaggtcga gcttgcgcat caggttgcgg cggtgcacct cgaccgtgga
    39841 ggcggcgatg tgcaggcgat cgccgatctg caccgaggtc aggccttcgg cgatcagctg
    39901 cagcacctgg cgctcgcgcg cggtgagccg gggtgcggcg gcgtacaccc cggggctgtc
    39961 gcgcagtgca tccgcgaccg tcgccgcgac ctcggggcac aggtagctgc gcccctcgca
    40021 cacggtgcgg atcgcgcgca gcagctcgtc gccggcttcg cccttggtga tgtagccgcg
    40081 cgcgccggcg gcgagcaggt cggtcacgaa ctggcggtcg gcgaaggccg aaaggccgat
    40141 cacgcgcagc gccggatggc gggcgagcag gcggcgggtg gcctcgatgc cgtccatgcc
    40201 cggcatggcg atgtccatgc acacgaggtc gaccggctgg gcatcggcga tcgcgagcac
    40261 ctcctcgcca ctcgccgcct cggcgaccac ttcgagctcc gggtcgcggg cgagcagcgc
    40321 ccgcagcgcg tggcggaaca tctggtggtc gtcggcaagc aggatgcgca ggctcatcgg
    40381 gtcgtttccg cggggtggag ctcggcgggc agccgcacct cgatgacggt gccgtcgccg
    40441 gggcgggtat gcaggcggaa gtccccgccg gcgagctcga cgcgctcgcg catcgtgatc
    40501 aggcccagcc ccggggtgct gcccgcctcg ccgagacggg agaggtcgaa gccgacgccg
    40561 tcgtcgccga tctgcagcag gaccccgccg gcgcagccgc cgagcccgat gcgcacgctg
    40621 ccggcgcgag cgtgcttggc gcagttggtg agcgcctcct gcactaggcg gaagcagagc
    40681 gtctgcgtcg cgcgggagag ggcgcacggg gggctgccgg acgcagcgtc gacgcgcacg
    40741 tccagcccgg tgcgggcgcg gaactgggcg acgtattcgc gcagtgcggg taccaggccg
    40801 gcgtagtcga gcgtggccgg gcgcaggttg gtgcagatct cgcggatgcc ggcggtggtg
    40861 tcggcgagca gggccgaggc gtcctccagc agcggggcga gctcatcgcg ggcggattcg
    40921 gggagggcgt cggccaggct ggagaacagg atctgcagcg cggcgaggtt ggggctggcg
    40981 cggtcgtgca gcgcgctggc gagttcgcgg cgttcgcgct cctcgatctc gacgaggtgg
    41041 cgggagagcg cctccaggcg ttccagatgc tccaggcgct cggactccag gcgccggcgc
    41101 tcggtgatgt cctgcaccgt gccgtacagg cggaacacgc gcccggtctc gtcggcgacc
    41161 ggcacgatca cggtgaaatg gcgacgctcc tcgcccgagg gcagcaggac ctcctgctcc
    41221 agggcgcagc gtcggccgct gtcgatcgcc tcccagaaca ggtcgcgggt gcgattcgtc
    41281 gactccaggc tgaagtagcc gagcgcctcg ttgaggtcgg gcacgccgag cgcttccggg
    41341 cgctcgaaga ggcggaagag ttcgcgcgac cagtacatcc gcccggtgtg gcggtcgagc
    41401 tcccagtcgc cgacacgggc gaggtcctgg gtggcggcga ggcggaactc cgccttctcg
    41461 agcgcgctgc gcaaggcttc gagccggggc gacctcattc gcgcaccgcc gtcctggcaa
    41521 gcgggcgcag cacgaagcgc gccggatcga tcgcctcgac caggtcgcgc tcgagcgggg
    41581 cggggtcgcc ttcgagcgcg ctggcgagca gctcggcggc gagcaccgac cacaccaggc
    41641 cgcgcgcgcc gtagcccgag agcgcgtaca gcccgggccg gcgcggcagc tcgtcgaggc
    41701 cgcgggcggc cgaggcggga ggccggtcgg gcagcgggag ggccccgacc atcggcaggc
    41761 ggtcgggtga ggcaggccgg aaccccaccc ggccatcgag ccgatcgggg tcgaagcccg
    41821 cgcccaggcc ggcgccgaag ccgggaatca tggcttccag gcgctgcagg ttctcggcat
    41881 ggtcctgcgg gcgcaggccg gggtcggggt cgtccaccgc gaaggtggcg ccggcgcagc
    41941 gcaggccgtc caccgccggg ctcacgtagc caccgcggca caccaccacc cgcggcgcgc
    42001 tgcccgcgac cgcgggcaga tgggagacct ggccgcgcgc gctcaccacc ggcaggctgg
    42061 cctgctcgaa gtcggcgatg ccggtgccgg gcgcgaggat cgccacgggg gcgctcgcga
    42121 tcgtgctgcc gtcgggcccg tgcgcgtgcc aggcgccgcc gtcgtacccg agccgcgcca
    42181 cggtgtggcc gaagtgccca cgcaggcgcc cgcccgccgc ctccaggctg gccgcacaca
    42241 ggctgggcgg ctgcacccag ccgctgccgg cgaaccacca gccgccgttg gcgaccgcgc
    42301 agccggcgat ttccgccgcc tcgtcgacgc cgacgaagcg caagtgttct ggcggcagcg
    42361 cgaggcgttc gaccaccgcg cgcatcttga cttcctgtgc agcgtcgcgc cccaggtgca
    42421 gcacgccgca gtcgtcggcg cgcagcgcca ggccgcgcac gcgcagctga tgaatgcgcc
    42481 gccaggcgta caggctgccg gcgcgggtga gccggctcat gcggttgtcg tccaggctgg
    42541 gcagcgggcg cagcacgccg gcgaggttgc ccgaggcgcc cgtggccggc gcaggggcac
    42601 ggtcgatcag ctccaccgtc cacccgcgcc cggcgaggcg ctcggccacg gtgcagccgg
    42661 caatgccggc accgagcacc agcgcgtggc gaccgctggt cgcggcctcc ggtacagcga
    42721 gcgggcgggc ctcgcgtgcg aggcggccgc gcagcatctg ccacttggtg ccgaagccgg
    42781 gcgccttttc ggtcgtgaag ccggcgcggc gcaggccttc gcgcacctgg ccggcgaccg
    42841 accaggtggc gagcgtcgcg tccggggcag cgaggccggc gagcagatgg aagacgcgct
    42901 cggaccacag cgcgggattc ttcgccgggg cgaagccgtc gagatagagc gcgtcggcgc
    42961 gcgcgtcgat gcgcgcgagg ccgtcggcgg catcgccgag gaagagcgtc agggtgacgc
    43021 ggcccgcgtc caggttcagt cggtgcagcc cgggcgcgag caccggccac tgcgcgcgca
    43081 gctcggccgc aaaggtggcg agctcgggcc aggcggcgtg gatgcgggcg agatcctcgc
    43141 ggcagaaggg gtgcagctcg caggagacga agtgcaggcg ggcgctgcgg caggcatcct
    43201 cgcgccacgc cgcccaggtg gcgaggaagt tgagtccggc gccgaagccg gtctcgacga
    43261 tggtgaagcg ctcacgcccc tgccagcgct gcggcaggcc gttgcccgca atgaagaccg
    43321 cgtgcgcctg gccgagcgca ttgtcgcgcg agtggtagac gtcgccgaag gcgcccgagt
    43381 acagcacgcc ctcgggcgtg cgctcgaggc gggcgggatg gatcggcatg gcgaggatgc
    43441 gcacgggccg ccgctgcgga gcgtggggac gctccgcgcg gcggacgccg ggatcagagc
    43501 tcgggttccg ggatgaccag gcccgcagcg gcctcggggc gcatctgcgg gaacaggatc
    43561 acgtcgcgga tcgccggcgc gtcggtgagc agcatcacca ggcggtcgat gccgatgccg
    43621 cagccgccgg tcgggggcag gccgtattcg agcgcgcgga tgtagtcggc gtcgtaatac
    43681 atggcctctt cgtcgccggc ctccttggcc tcgacctggg cgtggaagcg cgcggcctgg
    43741 tcctcggggt cgttgagctc ggagaagccg ttggcgatct cgcggccgac gatgaagagc
    43801 tcgaagcgct cggtgatctc ggggtcggtg tcggacgcgc gcgccagcgg cgagacctcg
    43861 accgggtagt cgatgatgaa ggtcggctcc cacagctcgg cctcggcgca ggcttcgaag
    43921 agctgcagct gcaggctgcc caggccgccc ggcttgacct tctcgccgaa ggcgacgatc
    43981 ttttccttca gccacgcggc gtcggccagt tgttccgcgg tgaagcccgg gtggtacttg
    44041 cggatcgcct gcacgatggt gaggcggtgg aagggcttcg acaggtcgag ctcgcggccc
    44101 tgatagacga aggattcggt gcccagcgcc tcgcgcgcgg cgtggcggat caggccctcg
    44161 gtgaagtcca tcagcgt
//
LOCUS       DAOQBS010000011        42276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_456_length_42276_cov_15.547192, whole genome shotgun sequence.
ACCESSION   DAOQBS010000011 DAOQBS010000000
VERSION     DAOQBS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 42276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42276
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_456_length_42276_cov_15.547192"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PKN25_02580"
     CDS             <1..812
                     /locus_tag="PKN25_02580"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNV89813.1"
                     /translation="VPRLKGARLFTDMVTVTGTENLMMAAALADGETVIENAAREPEV
                     VDLANCLVAMGAQISGAGSDVIRIRGVERLHGATHRIMPDRIETGTYLCAAAVTGGSV
                     RLTGTSSCYLDAVIDKLMDAGCEVVSERDAIRLTAPARLNAVSLRTAPYPAFPTDMQA
                     QFMAINAVAKGVAMIRETIFENRFMHAVELQRLGADIRIDGNTAVVQGVDRLQGATVM
                     ATDLRASASLVVAGLVAEGDTTIERIYHLDRGYERLEEKLAALGARVRRLG"
     gene            complement(930..1337)
                     /gene="rnk"
                     /locus_tag="PKN25_02585"
     CDS             complement(930..1337)
                     /gene="rnk"
                     /locus_tag="PKN25_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNV89814.1"
                     /translation="MKPSIIVSSSDLERLEGLLTLPAFRSRSDLDGLRAELERADVRE
                     PQDMPADVITMNSRARFVEEGTGREYELTLAYPKDANAEAHRVSIFSPAGSALLGLSA
                     GQSIDWKSADGKEIRLKVLEVTWQPEANGQFEL"
     gene            1490..2134
                     /gene="hisG"
                     /locus_tag="PKN25_02590"
     CDS             1490..2134
                     /gene="hisG"
                     /locus_tag="PKN25_02590"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNV89815.1"
                     /translation="MSSITLALSKGRIFEETLPLLAAAGIVPTDNPESSRKLIIGTNR
                     PDVRLVIVRATDTPTYVQYGAADLGIAGKDVLVEHGGAGLYQPLDLEIAKCRLCVAVQ
                     KGFDYAAATRPGGRIRVATKYMNAAKAHFAGKGMHVDLIKLYGSMELAPLVGLADAIV
                     DLVSTGGTLRANNLEEVEDIMPISSRLIVNQASLKLKRELLQPVLDAFAGAIQS"
     gene            2148..3455
                     /gene="hisD"
                     /locus_tag="PKN25_02595"
     CDS             2148..3455
                     /gene="hisD"
                     /locus_tag="PKN25_02595"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNV89816.1"
                     /translation="MSQTPIRRLAAREPEFLSTLDALLAFEAEADGRIDAAVTEILQA
                     VRTTGDAAVVEYTRRFDGLDVQSMVALELPRSELLLALDSLRPEQREALTIAADRVRV
                     YHERQKGESWEFTEADGTRLGQKVTPLDRVGLYVPGGRASYPSSVLMNAIPAKVAGVG
                     ELIMVVPTPRGEKNPLVLAAAAITGVDRVFTIGGAQAVAALAYGTQTIPQVDKIVGPG
                     NAYVAEAKRRVFGTVGIDMVAGPSEVLIISDGSGHADWVAMDLFAQAEHDELAQSILL
                     CTDAGFIDAVHDAIDRLLPTMPRRDTIARSLANRGALIHVDSLEQACALANRIAPEHL
                     ELALEDAEDWIAHIRHAGAIFVGHWAVEALGDYCAGPNHVLPTMRSARFSSPLGTYDF
                     QKRTSIVHISQAGAQHLGKVASILAHGEGLQAHARSAEMRLKV"
     gene            3527..4615
                     /locus_tag="PKN25_02600"
     CDS             3527..4615
                     /locus_tag="PKN25_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV89817.1"
                     /translation="MTLKFGWMAALALAAGVAVAAEEPILNVYNWNDYIAADTIPAFE
                     KASGIEVRYDLYDSNATLQGKLLTGGSGYDVVYPSVEYAGKQIQAGIFQPLDKSRLPN
                     LVHIDPLILEAVAVADPGNRYLVPYMWFTIGVAINVDKVMKALDGKLPDNAWDLLFDP
                     ALTARLKGCGIALMDEASDVIPAAMLDAGLDPVKMDPADIRAAVEHIRPVRKDIRSFN
                     TAPIEQMAKGSLCVAMMFSGDARIAARRAQIGGSEVRLQYLIPAVGAMMSVDVMAIPR
                     DAPHPHNAHRWIDAMMDPATVARISNETFYISANTAALALTDATLRDDPAINVPEAVK
                     RTLRAKPVLGKDVQRELTQALNRFKAAR"
     gene            4680..5789
                     /locus_tag="PKN25_02605"
     CDS             4680..5789
                     /locus_tag="PKN25_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV89818.1"
                     /translation="MARERVHLRIESLGKRFGDTWAVREVSLDIGEREIFALLGSSGC
                     GKSTLLRMIAGLETPSCGRILLDGEDLAAVPAHRRPTNMMFQSYALFPHMTVAGNVAF
                     GLRQLRPRMARAAIAARVDEMLELVQMRAYAGRRPHALSGGQQQRVALARSLAREPRL
                     LLLDEPLAALDKRIRQQTQFELVGLIRRVGVTCILVTHDQEEAMTMADRIGILSEGGL
                     VQVGTPGEIYERPNCRFSASFIGETNLFHGRIEGDRLVGGEFPQPIRFVATVALQQGG
                     EAWLSLRPECVRVHHRAAGDACVPEQPDDNAAPAEVAEIAYLGGRSIYHLRLPGGGSM
                     IASVPAAHWGEAAPPARGEPVRVSWPAPDGVLLLR"
     gene            5786..6730
                     /locus_tag="PKN25_02610"
     CDS             5786..6730
                     /locus_tag="PKN25_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNV89819.1"
                     /translation="MKARPASAQGHGRFWVGLLPWVWMLFFFVLPFALIVNVSLSTPD
                     LAVPPYVSPFAGAAVDGAPAFRPDPASYARLGGELAALGTLAADRPYLAAYANSLLLA
                     ALTTLCCLVLGYPFAYRLARAPEAMRNLLLMLVMLPFWTSFLLRVYAWMGLLDSSETG
                     LINQVLLGLGLVAEPLPLLYNTGAVLVGMVYSYLPFMVLPLYANLVKLDQRLPEAARD
                     LGARPATVFLRITLPLSSRGIVAGSMLVFIPAVGEFVIPDLLGGGSVMTIGRSIWDDF
                     GPNQDWPMAAAVAVLMVAMLIAPIVLLQRHGRGAGRDE"
     gene            6727..7548
                     /locus_tag="PKN25_02615"
     CDS             6727..7548
                     /locus_tag="PKN25_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNV89820.1"
                     /translation="MNGGGWGGRMWVVGVYAFLYVPILCLIVFSFTAGEITTRLDGLS
                     LRWYAALARDAELHAAVLLSLRVGALAATAAVVVGTAAAVILVRAGPYPGSRFFVGMM
                     SAPMVMPEVVVGLSLVLLFTHPALAVLGGRGVAAIWVAHTTLCAAYVAVLVASRLREM
                     DRALEEAALDLGCPPLKVFFVITVPVIAPALAAGWLLAFTLSMDDFVLAAMLSDPGST
                     TLPVLVFARLHHGLKPEINALATVIVVVVSIAVLLANRLLLAPPRASAPAAARGG"
     gene            complement(7555..8628)
                     /gene="hisC"
                     /locus_tag="PKN25_02620"
     CDS             complement(7555..8628)
                     /gene="hisC"
                     /locus_tag="PKN25_02620"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNV89821.1"
                     /translation="MSRFWSAVVHGLTPYVPGEQPKLDNLVKLNTNEHPCGPSPKVLE
                     AIRAATGDGLRLYPDPNADALKAALARHHGVRPEQIFVGNGSDEVLAHAFMALLKHER
                     ALWFPDITYSFYPVYCGLYDIQPRIVPLAEDFSIRVEDYLPRGDERAGAIIFPNPNAP
                     TGRLLALAEVERIVAANPEAVVLVDEAYVDFGGDSAIALVDRHPNLLVTRTFSKSRAL
                     AGLRVGYAVGDAALIEALERVKNSFNSYPLDRLAIAGAVASVEDEDYFRASCAQVIET
                     RARLVADLQALGFEVLPSAANFIFARHPTRDGAELTAELRRRAIIVRHFKAARIDQFM
                     RITVGTDAQCAILVAALREILAG"
     gene            8757..9344
                     /gene="hisB"
                     /locus_tag="PKN25_02625"
     CDS             8757..9344
                     /gene="hisB"
                     /locus_tag="PKN25_02625"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNV89822.1"
                     /translation="MRQAEVTRNTLETKITVRIDLDGSGQGRLDTGVPFFDHMLDQIV
                     RHGLFDLDIHCEGDTHIDDHHTVEDVGITLGQAFAKALGDKKGLRRYGHAYVPLDEAL
                     SRVVVDFSGRPGLHYFVNYTRARIGNFDVDLAREFFQGFVNHAGVTVHIDNLRGDNAH
                     HQCETVFKAFARALRMAAEHDERAAGTIPSTKGAL"
     gene            9406..10044
                     /gene="hisH"
                     /locus_tag="PKN25_02630"
     CDS             9406..10044
                     /gene="hisH"
                     /locus_tag="PKN25_02630"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNV89823.1"
                     /translation="MTSVAIIDYGMGNLRSVAKAIEHVAPGHEVFVTSDPARVAAAER
                     VVFPGQGAMPDCMRELDARGLRPAVRAAAAEKPFLGICIGQQMLFQHSAEGDVPGLGI
                     LSGEVVRFPETGMFAADGSRLKVPHMGWNEVRQCGPHPMWDGIPDGERFYFVHSYFVV
                     PAEASLTAAETDYGVRFTSAVARANIFAAQFHPEKSAAAGLRLLANFIRWQP"
     gene            10089..10838
                     /gene="hisA"
                     /locus_tag="PKN25_02635"
     CDS             10089..10838
                     /gene="hisA"
                     /locus_tag="PKN25_02635"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNV89824.1"
                     /translation="MLLIPAIDLKDGQCVRLKQGEMDDATVFSSDPGAMARHWLEAGA
                     RRLHLVDLNGAFAGKPKNGAAIRSITDVVGDDIPVQLGGGIRDLDTIEHYLDNGISYV
                     IIGTAAVKNPGFLHDACSAFPGHIIVGLDAKDGKVAVDGWSKLTGHDVVDLARKFEDY
                     GVESVIYTDIGRDGMLSGVNIEATVRLARALRIPVIASGGITDLRDIDALCAVEDEGI
                     MGAITGRAIYEGTLDFAAAQARADELEGARE"
     gene            10861..11619
                     /gene="hisF"
                     /locus_tag="PKN25_02640"
     CDS             10861..11619
                     /gene="hisF"
                     /locus_tag="PKN25_02640"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNV89825.1"
                     /translation="MLAKRIIPCLDVKSGRVVKGVNFVELRDAGDPVEIARRYDEQGA
                     DEITFLDITASSDDRDIILHVVEQVAEQVFIPLTVGGGVRVVEDVRRLLNAGADKVSM
                     NTAAVNNPQLVFEAASKVGSQCIVVAIDAKQTAPGKWEVFTHGGRNNTGLDAIEWARK
                     VESLGAGEILLTSMDRDGTKSGFDLALTRAVSDAVRIPVIASGGVGTLEHLAEGVSEG
                     RADAVLAASIFHFGQHTVREAKELMRARGIEVRL"
     gene            11616..12011
                     /gene="hisI"
                     /locus_tag="PKN25_02645"
     CDS             11616..12011
                     /gene="hisI"
                     /locus_tag="PKN25_02645"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNV89826.1"
                     /translation="MSASTRWLNEIKWDEHGLVPVIAQEAATGDVLMFAWMNREALQR
                     TAETGEAIYWSRSRRKLWHKGEESGHVQKVLDMRIDCDNDVVLLRIEQVGGIACHTGR
                     HSCFFQKYFADGRWEAVEPVLKDPQEIYK"
     gene            12008..12346
                     /locus_tag="PKN25_02650"
     CDS             12008..12346
                     /locus_tag="PKN25_02650"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNV89827.1"
                     /translation="MIDIEVLHRVSETLAARKKADPDSSYVSSLYQKGTDTICKKVAE
                     EAAETIMAAKDKDMLHLVWEVTDLWFHSMVLLAHHGLSVDDVLAEFRRREGVSGIDEK
                     KSRTAEGSRA"
     gene            12343..12690
                     /locus_tag="PKN25_02655"
     CDS             12343..12690
                     /locus_tag="PKN25_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNV89828.1"
                     /translation="MSDCVFCRIVRGEIPSKKVYEDEHVFAFHDIHPAAPVHVLVVPK
                     VHVDSMAHLDGEHEAAMGRLMVAAGRIAREQGCTDGFRTIVNTGRVGLQEVYHLHLHI
                     LGGPDPLPPMLKR"
     gene            12701..12937
                     /gene="tatA"
                     /locus_tag="PKN25_02660"
     CDS             12701..12937
                     /gene="tatA"
                     /locus_tag="PKN25_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HNV89829.1"
                     /translation="MGSFSIWHWLIVLVIVLLVFGTKKLRNIGSDLGGAVKGFKEGMK
                     EGDSAGAGDASQQKLAQGQTIEGEAREKVDQNRS"
     gene            13034..13525
                     /gene="tatB"
                     /locus_tag="PKN25_02665"
     CDS             13034..13525
                     /gene="tatB"
                     /locus_tag="PKN25_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HNV89830.1"
                     /translation="MFDFGFSELVVIGIVMLIVVGPERLPKVARTAGHLLGRVQRYVS
                     DVKSDIQREMQLEELKKLQEQVRHQAQELETSVRTGVAGVESGIGETVAEVRSILPES
                     PASASGTAAPAPAGAPTPVANPAAAPAPAGADATPARPAPQLELGLDSTPRTAPGADK
                     VKA"
     gene            13522..14319
                     /gene="tatC"
                     /locus_tag="PKN25_02670"
     CDS             13522..14319
                     /gene="tatC"
                     /locus_tag="PKN25_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNV89831.1"
                     /translation="MSEQQETFIAHLIELRDRLIRALLAIVIVFVCLMPWAGAIYDIL
                     AKPMMDTLPAGTNMIATGVVTPFFVPVKVTMMVAFVFALPWVLYQAWAFIAPGLYAHE
                     KRLALPLVLGSTLLFLLGMAFCYFFVFGTVFKFIAEFAPKSIVPAPDIEQYLSFVMSM
                     FIAFGITFEVPVAVILMVKAGIVDVAKLRESRPYVIVGAFVVAAIVTPPDVVSQFMLA
                     VPMCLLYELGIVLAGMLSRPSPQQEAAGETVQQLAGPAAADGEGEPR"
     gene            complement(14347..15498)
                     /locus_tag="PKN25_02675"
     CDS             complement(14347..15498)
                     /locus_tag="PKN25_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNV89832.1"
                     /translation="MRRLWLIFAQAVTVSVAVLFVLNTLKPEWLRKATPAAVVAILEA
                     PAGSERAPAAGSYAPAAQRSMPAVVHIFTSKGGRGQRHPLLDDPLFRHFFGERPDSAP
                     SESGLGSGVIVSPDGYVLTNNHVIETADAIEVALNDGRKFAARLVGRDPETDLAVLRI
                     DGAEALPAITFPAADSLAVGDVVLAIGNPFGVGQTVTMGIVSALGRSQLGINTFENYI
                     QTDAAINPGNSGGALVDSAGSLVGINTAIYSRSGGSLGIGFAIPVSIARDVLEQIVAT
                     GEVVRGWVGVEIQDLTPELAASFGYRDAGGALIAGVLRGSPADRAGVRPGDVLVALDG
                     ESVRDPRAMLDMVAALSPGKRAVFRVRRGAEALDLEVEVGRRPIPPAAR"
     gene            15594..16343
                     /locus_tag="PKN25_02680"
     CDS             15594..16343
                     /locus_tag="PKN25_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNV89833.1"
                     /translation="MQLTELGGHLDTLLDASRLKDYCPNGLQVEGRAEVRRVLCGVTA
                     SQALLDLAATGAYDAVFVHHGLFWKGDDGRVTGFRRRRLHTLLANDISLFAYHLPLDV
                     HPELGNNAQLARLMGWQGTARFADQELGWIGRPAREGERADEIARALARRLGREPLLV
                     GDGERTVRRIAWCTGGAQGFFEQAILAGAELYVSGEISEPTVHLARESGVPYIAAGHH
                     ATERYGARAMAHYLNEVLGLEASFVDLDNPV"
     gene            complement(16340..18145)
                     /locus_tag="PKN25_02685"
     CDS             complement(16340..18145)
                     /locus_tag="PKN25_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89834.1"
                     /translation="MNNNSMPLAIAVAIALASLGTAPPAAAVALGEVAALSAMGESFR
                     AEIRLPDGKPADAECFRVAPLSGGEFPALRQGRIAVSGSGAEARLVVRDAARVNEPVL
                     KLAIENVCEARLRREYTLLMPFAPGARLPAAAVAAAAPPPAARAPARTPRTAPPAQAK
                     AAPKPRARTLGTSGDESLVTLASSLYPDDPAAQARFIEATAAANPNLFADAAALTRVL
                     PAGTALRLPDLRPAAPQPEAAPAPVVAQGPDRLVVDKTVATRSTGGTTAAHGDAPPRN
                     HAERERALAAAIDRSIIAEMELLARIKELEEIQAGLKERLLRMQAVPAVATAPAVAVP
                     ASPPPRTEPVTPAEKRPALAAENAWGRDAYLFGGLGFAALALVALLRRSRRPTPAAPS
                     SRPTDTATATTLGSAESRKGRARAEETGLGFGVGTVDTRLEWPAGTATETIQEHKSAV
                     ELADIMMSFGRVRGAAETLAEFIRGNPREAVSPWLKLLEVYRAAGLRDEFNAIAGELN
                     KTFNVTTVNWQSYDALRASKMSLEDLPHICEALQKTWRTAACQRHLQQLLRDNRNGTR
                     AGFPFSVIDEILTLTAILEEDLGPCPPAADEPPRR"
     gene            18558..19718
                     /gene="sucC"
                     /locus_tag="PKN25_02690"
     CDS             18558..19718
                     /gene="sucC"
                     /locus_tag="PKN25_02690"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNV89835.1"
                     /translation="MKIHEYQAKELLRKYGVVTPRGFHCVSVDAAVKAAEELGGKIWV
                     VKAQIHAGGRGKGGGVKLARSLDEVRQHANDILGMQLVTHQTGPEGQKVRNLLIEEGA
                     DIKHEYYVAALTDRATQTVAMMASSEGGMDIEEVAHNTPEKIIKVFVDPLVGLTDAQA
                     AELAKGIGVPEGSVAKAVDAFKKLYTCYMETDASLAEINPLILEGNGNIKALDAKFNF
                     DSNALYRHPEIVDFRDFDEEDADEIEASKYDLAYISLDGNIGCLVNGAGLAMATMDTI
                     KLFGAEPANFLDVGGGATTEKVTEAFKIMLKNPQVKGILVNIFGGIMRCDTIATGVVA
                     AAREVNLSVPLVVRMKGTNEDLGKKILADSGLPIIAADTMAEAATKIVAAVK"
     gene            19731..20624
                     /gene="sucD"
                     /locus_tag="PKN25_02695"
     CDS             19731..20624
                     /gene="sucD"
                     /locus_tag="PKN25_02695"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNV89836.1"
                     /translation="MSILINKDTKVITQGITGKTGQFHTEKCIEYANGKNCFVAGVNP
                     KKAGEKILDVPIYGSVKDAKDATGATVSVIYVPPAGAAAAIWEACEADLDLAICITEG
                     IPVRDMLVVRNRMKQKVAAGGKETLLLGPNCPGLITPDEIKIGIMPGHIHRKGRIGVV
                     SRSGTLTYEAVAQLTEIGLGQSSAVGIGGDPINGLKHIDVMRMFNDDPDTDAVIMIGE
                     IGGPDEAEAAQWCKANMKKPIVGFIAGVTAPAGKRMGHAGALISGGADTADAKLAIME
                     ECGFKVTRNPSEMGKLLKAML"
     gene            20771..21517
                     /locus_tag="PKN25_02700"
     CDS             20771..21517
                     /locus_tag="PKN25_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNV89837.1"
                     /translation="MPKLVLSMDGLVLKEMALVKERTTIGRKPDNDIQIDNLAISGHH
                     AVITCITNDAFLEDQNSTNGTYLNGQPVKKNVLRNNDVIELGKYRIKYIVDDAKPGLA
                     PSEMIETAAYKAFEMPRATTETVLPPGAPAPSDTQILSAAARQAGTAAAAATAASAAG
                     EGQGVIQVLSGANAGRELPLTKSLTTLGKPGRQVAVITRRPHGYFITHVEGASFPLVN
                     GRAIDAQARPLRDHDIIEIAGVKMEFFLRA"
     gene            21632..22399
                     /locus_tag="PKN25_02705"
     CDS             21632..22399
                     /locus_tag="PKN25_02705"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNV89838.1"
                     /translation="MKLKVLGCSGGIGGAQPRTTSFLLDDDILIDCGTGVGDLTLDEL
                     ARIDHVFLTHAHLDHIAALPLMIDSVGELRQAPVQVHATPETIRILHSHIFNWLVWPD
                     FSAIPDHHQPFMRFHPIHVGERRDCGGRGITALPARHTVPAVAYRLDSAHGQLVFSGD
                     TAYCPELIEAINDCPQLRHLILETAFAEEQHGLAVASRHLCPSMARAVLDEIRGTPEI
                     HISHLKPGVGQRIIDQLGACAPGRPLRALGRGDLLEF"
     gene            22482..24827
                     /locus_tag="PKN25_02710"
     CDS             22482..24827
                     /locus_tag="PKN25_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNV89839.1"
                     /translation="MNTSVSSAVTHRLAFFKGLQTLTNRIHASKDTDEIMLELAPELC
                     SLFEAERFTIYALEEGGASIVTRVKTGLSGVQSIRLPIGENSIAGFVALSGQLVNIRD
                     VYDDEELRRISPHLALRREVDASTGFHSRQMLVAPIRDPDTGSLLGVVQLINTLNREP
                     FSKMTEEGLMGLAQTLGVAFLRHNRQAGRLRSRFEALVVDGRITAEEFADATREARES
                     GAGTENLLIEQLGLSHAELGAAASRYFGVPYQPFVANHVKPMDLLRNIKREFVERSLW
                     LPWEENPEGVVILCVDPEQVRSSGVAQNVLPKKRLTYRVTTQRDFELTVEQFFGVTLD
                     DASVSDLLSGLDDHEEDALSLRDEVNAAADNELVKLVNKIIIDAYRQGASDIHIEPRP
                     GKEKTQIRFRKDGSLVPYIEIPASYRNPLVTRIKIMCDLDISERRKPQDGKIRFRKFA
                     PVDLELRVATLPTQGGMEDVVMRLLAHSEPIPIDQLGLLPFNLERLKSAIAKPYGIFF
                     VCGPTGSGKTTTLHSILSYINTPDTKIWTVEDPVEITQKGLRQVQVNRKAGIDFATMM
                     RAFLRADPDVIMVGEMRDHETVSVGIEASLTGHLVFSTLHTNSAPESVVRLLDMGMDP
                     FNFADALLGVLAQRLAKRLCLKCRVPYRVGEDELASLLDEYCEDMHLTPDFAADPKTA
                     RERVLGRWREAFGDKDGGFRLYRAVGCEHCNQGYKGRVGLHELMLGSDAIKRQIQSRG
                     RVDQILGQALAEGMRTLRQDGIEKVLAGITDMKQVRKVCVR"
     gene            24898..25593
                     /gene="rpe"
                     /locus_tag="PKN25_02715"
     CDS             24898..25593
                     /gene="rpe"
                     /locus_tag="PKN25_02715"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNV89840.1"
                     /translation="MYRIAPSLLSADFARLGEEVRDVIAAGADWIHFDVMDNHYVPNL
                     TIGPLVCEAIRPHTSAPIDVHLMVKPVDRIVPDFAKAGANVITFHPEASEHVDRTLGL
                     IRDAGCQAGLVFNPATPLDWMDHVMDKLDVVLLMSVNPGFGGQKFIPETLNKLRAARA
                     RLDAYEARSGRRILLEIDGGVKTDNIAAIARAGADTFVAGSAVFGAAKAADPHRYDSI
                     LGALRAELAAVQA"
     gene            25596..26270
                     /locus_tag="PKN25_02720"
     CDS             25596..26270
                     /locus_tag="PKN25_02720"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNV89841.1"
                     /translation="MDARFAPRAVLFDLDGTLLDTIADLADAANLMLAELARPARSLE
                     EIHSFVGKGIPNLVRRCLTEGTHADEDEIDSAVAVFRRHYAVVNGARTRIYPGVGELL
                     ERLRGHGLPLAVVTNKAEAFTLPLLERMAIAHYFDAVVSGDTLPVKKPDPAMLHLACA
                     RLGVAARQALMVGDSANDAFAARDAGMPVLLVRYGYSEGMPVDSIECDGLLSNALEVL
                     DHIGPA"
     gene            26584..28056
                     /gene="trpE"
                     /locus_tag="PKN25_02725"
     CDS             26584..28056
                     /gene="trpE"
                     /locus_tag="PKN25_02725"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNV89842.1"
                     /translation="MLEHEFNALAAEGYNRIPLTLETFADLDTPLSIYLKLANEPYTY
                     LLESVQGGERFGRYSFIGLSSPTRIEVYGRSALLLTGNRLVERRDYGDPLNFVAEFMN
                     RIKVPPRQHLPRFAGGLVGCFGYDTVRYIEPRLAKTEKADTVGTPDILLLLSEEVAIV
                     DNLSGKLTLVVYAEPEVPGAYKRAQRRLRELLARLREAVEIPEEFRGESAEPVSSFGE
                     EAFKDAVRRAKQYIVDGDLMQVVLSQRMSKPYAASPMALYRAIRSLNPSPYLFYFNLE
                     DFHVVGASPEILTRLEDDVVTVRPIAGTRKRGATPAEDLALEQELLADQKEIAEHVQL
                     LDLGRNDAGRVSEVGTVRVTERFTVERYSHVMHIVSNVEGKLREGLDALAVLRATFPA
                     GTVSGAPKVRAMEIIDELEPVKRGIYAGAVGYLGFHGDMDLAIAIRTAVVKDGQIHVQ
                     AGAGIVADSDPDAEWNETQSKARAMLRAAEMAEGGLDTRF"
     gene            28142..28729
                     /locus_tag="PKN25_02730"
     CDS             28142..28729
                     /locus_tag="PKN25_02730"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNV89843.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGAKVKVYRNDEITLEQIGRLAP
                     EHLVISPGPCSPAQAGISVAAIKAFAGKLPILGVCLGHQSIGEAFGGRVVHAKRLMHG
                     KTSPVHHIDQGVFRGLPNPLTCTRYHSLAIERASLPDCLEVTAWTEDGEIMGVRHRTL
                     DVEGVQFHPESILTECGHALLKNFLDRGASQPAAA"
     gene            28751..29794
                     /gene="trpD"
                     /locus_tag="PKN25_02735"
     CDS             28751..29794
                     /gene="trpD"
                     /locus_tag="PKN25_02735"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNV89844.1"
                     /translation="MTTITAQQALQRTIEHREIFFDEMLSLMRQIMAGEISPVMTAAI
                     LTGLRVKKETIGEITAAATVMREMSTKVVVEPPHDHFLDVVGTGGDGANTFNISTTTI
                     FVAAAAGARVAKHGGRGVSSKSGAADVIEALGVNLDLTPQQVAECIEATGIGFMYAPA
                     HHSAMKNVAPVRREMGVRTIFNILGPLTNPAGAPNTLMGVFHPDLVGIQARVMQRLGA
                     NHVLVVHGLDGMDEVSLGGATLVGELKDNKVVEYEIHPEDFGLAMAGSRQLRVEDAAE
                     SREVLLGVLDNRPGPAREIVAFNAGVALYTANLVDTIGAGILRAREIIESGAARAKLD
                     EFVRFTRQFGSGR"
     gene            29791..30591
                     /gene="trpC"
                     /locus_tag="PKN25_02740"
     CDS             29791..30591
                     /gene="trpC"
                     /locus_tag="PKN25_02740"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNV89845.1"
                     /translation="MSDILQKICAVKVEEVAAAAAARPQCLVREDAAAQPPARDFVGA
                     IRAKHAARRPAVIAEIKKASPSKGVIRPDFHPAEIAADYERAGAACLSVLTDRGFFQG
                     APEYLQAARAACHLPVLRKDFLVDPYQVFEARAMGADAILLIAACLDLARMRDMEQLA
                     VELGMAVLVEVHDAAELEHALQLETPLIGINNRNLRTFEVSLQTTLDLLPRIASGAAG
                     RIVVTESGILKPEDVALMQANAVHTFLVGEAFMRAPHPGAELQALFGR"
     gene            30601..34887
                     /locus_tag="PKN25_02745"
     CDS             30601..34887
                     /locus_tag="PKN25_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNV89846.1"
                     /translation="MSAHDHDASPAPAAPPRRRRLARALGWGVALPLALLCGGGAWLA
                     GTQSGLDAALGLAARATSGALQVEGARGRLFGRLDVDRLAWRGPGLELEVRALSLAWT
                     PAALLQRRVDIEHLTAGRVLLAQAVAEDPPASAPLAAPSSLALPVAIALRRLAVGAFL
                     MRELPAGGDLAVPLAGAAPGSADAAVPVQGEVAAAAGEAAQALDRAADVASDPALAAE
                     AATEVAAEQGLVFSRLEAALSSDGRHHRLESLSVALPQGRARAALALDGVAPFALAGE
                     AGFDGVVETHAVAVQATLADSLLEPRLQAKATLGAAHAQIEARVESFAADPLRQLRLK
                     AGGIDPAAFAAGAPSGEFAIEAELAAPAAGEGLLAGPIRVTNARPAAVDAGGIPLEAL
                     SAQLDWKRAELALDAVEFRLAGGGTIAGRVAWHPPALPEATASAAPAAEAGFGQFVAA
                     LELAGIDTRALDGRLPRQIVAGRVDAEAGAQRQTARLALMVGAARIDADASLSVVPAA
                     PPSPVAAASAAGEGGAQTAREGAAAPPVPAPATDLPAAVHAGAARSFTLSARLRGVDP
                     RALRSELPSARLDLDLDARGDLSAEGLPQAVALDFTLPDSRLDGRPLRGEGRLRVEGL
                     RVPEAKIDLNIAGNTLRAEGAFGAPGDSLALRLDAPALAAIGFGLGGRAGAEGRIGGT
                     LEAPNGRLDLFGENLALPGDVRLAGLNARVRLDSGIDGPFELALGLSRLGSRARGADG
                     APPPDWLASARIEAGGTRARHRLDADLATPKVEGVADTLRLALEGGLPAGEAPRWSGR
                     LAALEVGGRFPARLQAPATLELGADRVRLGRVEIEAGDKGRIRLLETAWSPTQSVLRG
                     DLAGLVVETEPRRRDGRPRRNADPLSLGARWDLAVGRTLEGEARVFRESGDISVEGEL
                     RTRLGLEKLEAVLNARGDRIDLAVDVQGKELGSVQGEAGLRARRGADGLWSVPPATAI
                     RGAARIDMPSLAWLGRLSQESVELAGRIRGDFTVGGTIESPRASGRIEGRELGFTLVD
                     QGLMLAGGELDVDFDQERVRLERLEFVSENRVRPRENRIPFERLTATPGRFTARGELA
                     LDSGAGDFRFDADRLPLLQRDDRWMLLSGGGTARSTFESLALDAEFRADAGYLEFAEA
                     LPPSLSDDVVVLGREPAPAQQGGGFAVNADVSVGLGDALYLSALGLETRLAGGLRIRL
                     QPGRPLSAVGTIETVGGGYRGYGQNLTLERGLINFQGPLDAPGLNIVALRKGLEVEAG
                     VAITGSAKRPQIKLVSDPEVPDPTKLSWIVLGRAPDAGSGADLGLLLPAAAALLGGPG
                     GGMTEELSRSLGFDSFSIGQGELNSTTRTRSSRVLGSGQTVSGGPSVAGQVLSLGKRL
                     GPDLFLNFEQGLGGAATLVKLTYQVSRRLSVIARGGTDTALDLQYGFSFR"
     gene            34966..35535
                     /gene="msrA"
                     /locus_tag="PKN25_02750"
     CDS             34966..35535
                     /gene="msrA"
                     /locus_tag="PKN25_02750"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNV89847.1"
                     /translation="MTESKNDPVRETAILGGGCFWCLEAVFDLVAGVDEVVPGYCGGH
                     VELPSYRDVCSGGTGHAEVVKIVFDPAKVSYRELLDVFFTIHDPTTVDRQGNDVGPQY
                     RSVIFATSEAQLHTALATIEALGERRVFPKAIVTRVERAPRFWPAEPEHHDYYVNNSD
                     QPYCQYVVAPKITKFRSHFDLRRWPEGSR"
     gene            35569..35913
                     /locus_tag="PKN25_02755"
     CDS             35569..35913
                     /locus_tag="PKN25_02755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNV89848.1"
                     /translation="MTQTTTASGLVIDELELGSGDTAEKGRMVSVHYTGWLTDGRKFD
                     SSKDRNDPFVFPLGAGHVIRGWDEGVQGMQVGGKRKLTIPPELGYGARGAGGVIPPNA
                     TLVFEVELLKVL"
     gene            36014..36472
                     /locus_tag="PKN25_02760"
     CDS             36014..36472
                     /locus_tag="PKN25_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004386182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNV89849.1"
                     /translation="MKLDPAQRHILTLLQKDSTLSTQALADKVGMSPTPCWRRVREME
                     EAGVIRGYVALVDRHKVGLGTCVWVRVKLKQHSADVLDRFERVVKACEQVVECYELLG
                     ETDCLLKLYLPNLEALSVFMHEFLLKIPEIDVTHSAVALREIKNETALPL"
     assembly_gap    36696..36795
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(36850..38190)
                     /locus_tag="PKN25_02765"
     CDS             complement(36850..38190)
                     /locus_tag="PKN25_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNV89850.1"
                     /translation="MAKHAHSQWSSRMGFVLAATGSAVGLGNIWKFPYMVGQSGGAAF
                     VLVYLACIAFIGVPILVAEWMIGRRGQKNPINTMAQVARDNGHSTNWAVVGAIGVLAA
                     FLILSFYSVIGGWALAYMRDAATGAFIGLDKAAIGGAFEGFLARPAELLTWHSIFMLL
                     TVVVVALGVSAGLERGTKLMMPALGVILLVLVGYAMTTGSFGQGLAYLFNPDWSKLDG
                     KVLLAALGHAFFTLSLGMGIMMAYGSYLGQEVNLLRAARTVVIMDTVFALCAGMAIFP
                     IVFANGLDPAAGPGLVFVTLPLAFGHMGGGLVIGALFFLLLTFAALTSSISLLEPVVE
                     LIEERTPLGRVAATLIAGITIWALGIAALLSFNVWSDVKLLGMNIFDLLDYATSKFML
                     PLAGLGAIVFAAWKLDQQGVKAELGLGDATFGLWTLLSRYVAPVGVLFVFWSNL"
     gene            38666..42262
                     /locus_tag="PKN25_02770"
     CDS             38666..42262
                     /locus_tag="PKN25_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNV89851.1"
                     /translation="MSNIAPTVSLDDKYTLKTGRAWMTGIQALVRLPMMQKLRDEAAG
                     LNTAGFVTGYRGSPLGSVDQEFWKAKRFLEPMNIRFQPGLNEDLAATSVWGTQQLGLD
                     PNAKVDGVFSIWYGKGPGVDRTCDVFRHANAAGTSQHGGVLVIAGDDHAAKSSTLPHQ
                     TEHVFKALMMPVLAPAGIQEYLEYGLHGFAMSRYSGCWVAFKALTDTVETSSSVDIDP
                     FRVDIRVPSAQEFPLPLDGLNLRWPDPPLVQEERLLHHKLYAALAYGRKNGLDRMVID
                     PPQARLGILTSGKSYLDVRQALDDLGIDEATAAAIGLRLYKVGMVWPLDAEGVRRFAD
                     GLDEILVIEEKRQFLEYQLKEELYNWKDEVRPRVIGKFDEKGEWSLPHADWLLPAAGE
                     LTPAMIARAIAGRIARFHTSDRIKARLAFIEAKEAALARPRLSIQRVPHYCSGCPHNT
                     STKVPQGSRAIAGIGCHYMATWLSPEHTQTFCQMGGEGVPWIGQAPFTNTPHVFANLG
                     DGTYMHSGILAIRAAVAAKVNITYKVLYNDAVAMTGGQPHDGTLSVPIIARQLLAEGV
                     GTVVVVNDGTPRAYGPADLPHGVAVRHRDELDAVQRELRTAPAVTAIIYDQTCAAEKR
                     RRRKRGKFPDPAKRVVINDLVCEGCGDCSDKSNCMSVGAVETEFGRKRFIDQSSCNKD
                     YSCVKGFCPSFVTVEGGKLRRGKASGASALGGRLADELPEPQLPDTAAPWGILITGVG
                     GTGVVTIGALLGMAAHLEGKGISVLDMAGLAQKGGAVWSHVRIADRQEQLHAARVAAG
                     EANAVIGCDLVVAASDESLAKMQHGLTRVLINRDQTNTSEFVRGFAAQARSGDVLANP
                     DPQFPAAAMEAQIVDAVGADKAEFLDATALATELLGDSIATNLFMLGFAWQRGLVPLS
                     RQAIERAIEINGTAIEANKAAFLWGRRAAVDLRAVREAAKPRFGTPAHHRLSTSVDEL
                     IARRKTHLTDYQDAAYAERYLRLVERVRVAEAKVAPGITLLTEAVARGYHKLLAYKDE
                     YEVARLYTDSDFLARVEEQFEGDYKLVFHLAPPMLADKDPASGELKKKAYGPWMLKAM
                     RVLAKLRHLRGSRLDPFARSHDRKLDRELIADYERVVDEIVVNLDRANHEAAIELAAL
                     PEQIRGYGHVRERYLAGARRRQAALLEDFRHRRAPGTPLATKARKTIAIIPG"
BASE COUNT         6427 a        15045 c        14470 g         6234 t
ORIGIN      
        1 ccgtgccgcg cctgaagggc gcgcgcctgt tcaccgacat ggtgacggtg accggcaccg
       61 agaacctgat gatggccgcg gcgctggccg acggtgaaac cgtgatcgag aacgccgcgc
      121 gcgagcccga ggtggtcgat ctcgccaact gcctggtggc gatgggtgcg cagatctcgg
      181 gcgccggcag cgacgtgatc cgcatccgcg gcgtcgagcg gctccacggc gcgacccacc
      241 gcatcatgcc cgatcgcatc gagaccggta cctacctgtg cgcggcggcg gtcaccggtg
      301 gttcggtgcg cctgaccggc acctcgtcct gctacctcga cgcggtgatc gacaagctca
      361 tggacgccgg ctgcgaggtg gtgtccgagc gcgacgcgat ccgcctgacc gcgccggcac
      421 ggctcaatgc cgtgagcctg cgcaccgcgc cttatccggc cttcccgacc gacatgcagg
      481 cgcagttcat ggcgatcaat gcggtcgcca agggcgtggc gatgatccgc gagaccatct
      541 tcgagaaccg cttcatgcac gcggtcgagt tgcagcgcct cggcgccgac atccgcatcg
      601 acggcaacac cgcggtggtg cagggcgtgg acaggctgca gggcgcgacc gtgatggcga
      661 ccgacctgcg cgcgtcggcg agcctggtcg tagccggcct ggtggcggag ggcgacacca
      721 cgatcgagcg catctaccac ctcgatcgcg gctacgagcg tctggaggag aagctcgccg
      781 cgctcggggc gagggtgcgg cggctcggtt gagaggagtt tgagagctgc gacggcggca
      841 tgcgctcgcg tgcacgcggc cgtcgtgccg tcgccgaact gcgacttgcg tcgctcccgc
      901 agggcagccg cggaacgctg cgggagcgct tacagttcga actgtccgtt ggcttcaggt
      961 tgccaggtga cttccagcac cttcaggcgg atttccttgc cgtcggccga cttccagtcg
     1021 atcgactggc cggccgaaag accgagcagg gcgctgcccg cgggcgagaa gatcgacacg
     1081 cggtgcgcct cggcgttggc gtccttgggg taggccaggg tcagctcgta ttcgcggccg
     1141 gtaccttcct cgacgaagcg cgcgcggctg ttcatcgtga tgacgtcggc cggcatgtcc
     1201 tgcggctcgc gcacgtcggc acgctcgagt tccgcgcgca ggccgtcgag gtcgctgcgg
     1261 ctgcggaagg cggggagggt gagcagaccc tcgaggcgct cgaggtcgct gctggagacg
     1321 atgatcgacg gtttcatggt gcgggtttcc tgcctgaggt gcgcgccgac gctgcggcga
     1381 aacaaaagaa aaggccgaac cgccgcgtgc ggttcagcca gtcgcgtcga aggttaacag
     1441 aagcggcgcc gggttacaat gacgagtccc cccgatcaag gcttttcacg tgtccagcat
     1501 cacgctcgcc ctgtccaagg gccgcatctt cgaagagacc ctgccgttgc tcgccgcagc
     1561 gggcatcgtg cccactgaca accccgagtc ctcgcgcaag ctgatcatcg gcaccaaccg
     1621 gccagatgtg cgcctggtca tcgtgcgcgc caccgatacg cccacctacg tccagtacgg
     1681 cgccgccgac ctcggcatcg ccggcaagga cgtgctcgtc gagcacggcg gtgcgggtct
     1741 ctaccagccg ctcgacctcg agatcgccaa gtgccggctg tgcgtggcgg tgcagaaggg
     1801 cttcgactac gccgccgcga cccgcccggg cgggcgcatc cgcgtcgcca ccaagtacat
     1861 gaacgcggcc aaggcccact tcgccggcaa gggcatgcat gtggacctga tcaagctcta
     1921 cggttcgatg gagctcgcac ccctggtcgg gctcgccgac gccatcgtcg acctggtgtc
     1981 gaccggcggc acgctgcgcg ccaacaacct cgaagaggtc gaggacatca tgccgatcag
     2041 ctcgcgcctg atcgtcaacc aggcctcgct caagctcaag cgcgaactgc tgcagccggt
     2101 gctcgacgcc ttcgccggcg cgatccagtc ctgaggagtc cctaccgatg agccagaccc
     2161 cgatccgccg cctggccgcg cgcgaacccg aattcctgtc caccctcgat gccctgctcg
     2221 ccttcgaggc cgaggccgac ggccgcatcg acgcggcggt caccgagatc ctgcaggcgg
     2281 tgcgcaccac cggcgacgcc gcggtggtcg aatacacccg ccgcttcgac gggctcgacg
     2341 tgcaatccat ggtcgcgctc gagctgccca ggagcgagct gctgctcgcg ctcgacagcc
     2401 tgcgccccga gcagcgcgag gcgctcacca tcgccgccga ccgcgtgcgc gtctatcacg
     2461 agcgccagaa gggcgagtcg tgggaattca ccgaggccga cggcacccgc ctgggccaga
     2521 aggtcacccc gctcgaccgc gtcggcctct acgtgccggg cgggcgcgcc tcctacccga
     2581 gctcggtgct gatgaatgcg atcccggcca aggtcgccgg tgtcggcgaa ctgatcatgg
     2641 tcgtgcccac cccgcgtggc gagaagaatc cgctggtgct ggcggcggcg gcgatcaccg
     2701 gcgtcgaccg cgtgttcacc atcggcggcg cgcaggcggt ggcggcgctg gcctacggca
     2761 cgcagaccat cccgcaggtg gacaagatcg tcggcccggg caatgcctac gtggccgagg
     2821 ccaagcgccg cgtgttcggt accgtcggca tcgacatggt cgccggcccg tctgaagtgc
     2881 tgatcatctc cgatggctcc ggccacgccg actgggtggc aatggacctc ttcgcccagg
     2941 ccgagcacga cgagctcgcg cagtccatcc tgctgtgtac cgacgccggc ttcatcgacg
     3001 cggtgcacga cgcgatcgac cgcctgctgc ccaccatgcc gcgccgcgac acgatcgcca
     3061 ggtcgcttgc caaccgcggc gcgctgatcc acgtcgacag cctggagcag gcctgcgcgc
     3121 tcgccaaccg catcgcgccc gagcacctcg agctggcgct ggaagatgcc gaggactgga
     3181 tcgcccacat ccgtcacgcc ggcgcgatct tcgtcggcca ctgggcggtg gaggcgctcg
     3241 gcgactactg cgccggcccc aaccacgtgc tgccgaccat gcgcagcgcg cgtttctcct
     3301 cgccgctcgg cacctacgac ttccagaagc gcaccagcat cgtccacatc tcgcaggcgg
     3361 gcgcgcagca cctgggcaag gtggcttcca tcctggccca tggcgagggt ctgcaggcgc
     3421 acgcccgctc ggcggagatg cggctgaagg tttgacgcct gcccgcccgg gtcccccgac
     3481 ccgggcgggc aggcggtgtt cccttgtcga tcgcggggag gcgggcatga cgctgaagtt
     3541 cggctggatg gcggcgctgg ccctggccgc cggggtggcg gtggcggccg aggagcccat
     3601 cctcaacgtc tacaactgga acgactacat cgccgccgac accatccccg ccttcgagaa
     3661 ggcgagcggg atcgaggtgc gctacgacct ctacgacagc aacgccacgc tgcagggcaa
     3721 gctgctgacc ggcggcagcg gctacgacgt tgtgtacccg agcgtggagt acgccggcaa
     3781 gcagatccag gccggcatct tccagccact cgacaagtcg cgcctgccca atctcgtcca
     3841 tatcgacccg ctgatcctcg aggcggtggc ggtcgccgac cccggcaacc gctacctcgt
     3901 accctacatg tggttcacca tcggggtggc gatcaacgtc gacaaggtca tgaaggcgct
     3961 cgacggcaag ctgcccgaca acgcctggga cctgctcttc gaccctgcgc tgaccgcgcg
     4021 cctgaagggc tgcggcatcg cgctgatgga cgaggccagc gacgtgatcc ccgcggccat
     4081 gctcgacgcc ggcctcgacc cggtgaagat ggacccggcc gacatccgcg ccgcggtcga
     4141 gcacatccgc ccggtgcgca aggacatccg cagcttcaac accgcgccca tcgagcagat
     4201 ggccaagggc agcctgtgcg tggcgatgat gttctcgggc gacgcccgca tcgccgcgcg
     4261 gcgcgcgcag atcggcggct ccgaggtcag gctgcagtac ctgatcccgg ccgtgggcgc
     4321 gatgatgtcg gtggacgtga tggcgatccc gcgcgacgcg ccgcacccgc ataacgccca
     4381 ccgctggatc gacgcgatga tggacccggc cacggtggcg cgcatctcca acgagacctt
     4441 ctacatcagt gccaacaccg cggcgctcgc gctcaccgac gccacgctgc gcgacgaccc
     4501 ggcgatcaac gtgccggagg cggtcaagcg tacgctgcgc gccaagccgg tgctcggcaa
     4561 ggacgtgcag cgcgagctga cccaggcgct gaaccgcttc aaggcggcac gctgagcggc
     4621 gggcgggcgc agcgggtgat gcgatgagcg agcgcatgcc gcaggatggg cccgaaagct
     4681 tggcccgcga gcgcgtccat ctgcgcatcg aaagcctggg aaaacgcttc ggcgacacct
     4741 gggcggtgcg cgaggtctcg ctcgacatcg gcgagcgcga gatcttcgcg ctgctgggca
     4801 gctcgggctg cggcaagtcc accttgctgc gcatgatcgc cggcctggag acgccgagtt
     4861 gcgggcgcat cctgctcgac ggcgaggatc tcgccgcggt gcccgcgcac cgtcgtccga
     4921 ccaacatgat gttccagtcc tacgcgctgt tcccgcacat gacggtggcg ggcaacgtcg
     4981 ccttcggcct gcgccagttg cggccgcgca tggcgcgcgc ggcgatcgcg gcccgcgtcg
     5041 acgagatgct cgagctggtg cagatgcgcg cctacgccgg gcgccgccca cacgcgctct
     5101 ccggcggaca gcagcagcgc gtggcgctgg cgcgcagcct ggcgcgcgag ccgcgcctgc
     5161 tgctgctcga cgagccgctc gccgcgctcg acaagcgcat ccgccagcag acccagttcg
     5221 agctcgtcgg cctgatccgg cgtgtggggg tcacgtgcat cctcgtcacc cacgaccagg
     5281 aggaggcgat gaccatggcc gaccgcatcg gcatcctgtc cgagggcggg ctggtgcagg
     5341 tcggcacgcc gggtgagatc tacgagcgcc ccaactgccg cttcagcgcg agcttcatcg
     5401 gtgagaccaa cctcttccac ggccgcatcg agggcgatcg cctggtcggc ggcgagtttc
     5461 cgcagccgat tcgcttcgtg gccaccgttg cgctgcagca ggggggtgaa gcctggctgt
     5521 cgctgcgccc cgagtgcgtg cgcgtgcatc atcgcgccgc cggcgatgcg tgcgtgccgg
     5581 aacagcccga tgacaacgcc gcgccggccg aggtcgccga gatcgcctat ctcggcggcc
     5641 gctccatcta ccacctgcgc ctgcccggcg gtggctcgat gatcgccagc gtgcccgcgg
     5701 cgcactgggg cgaggccgcg ccgccggcgc gcggcgagcc ggtgcgggtg tcgtggccgg
     5761 cgcccgacgg cgtgctgctg ctgcgatgaa ggcgcgtccg gcttctgcgc aagggcatgg
     5821 ccgcttctgg gtcggcctgc tgccgtgggt gtggatgctg ttcttcttcg tgctgccctt
     5881 cgcgctgatc gtgaacgtca gcctgtccac gccggacctc gccgtgccgc cctacgtgtc
     5941 gcccttcgcc ggcgcggctg tggacggggc gccggccttc cgtcccgatc ccgccagcta
     6001 cgctcgcctc ggcggcgagc tggcggcact cggcacgctc gccgcggacc ggccctacct
     6061 cgccgcctac gccaattcgc tcctgctcgc agcgctcacc acgctgtgct gccttgtcct
     6121 cggctatccc ttcgcctatc gtctcgcgcg cgcgcccgag gcgatgcgca acctcctgct
     6181 gatgctggtg atgctgccgt tctggacctc cttcctgctg cgcgtctatg cgtggatggg
     6241 tctgctcgac agcagcgaga ccgggctgat caaccaggtg ctgctcggcc tcggcctggt
     6301 ggcggagccg ctgccgctgc tctacaacac cggcgcggtg ctggtcggca tggtctacag
     6361 ctacctgccc ttcatggtgc tgccgctcta cgccaatctg gtcaagctcg accagcgcct
     6421 gcccgaggcc gcgcgcgacc tgggcgcgcg gccggccacg gtgttcctgc gcatcaccct
     6481 gccgctgtcc tcgcgcggca tcgtcgccgg ctcgatgctg gtgttcatcc cggcggtggg
     6541 tgagttcgtc atccccgatc tgctcggcgg cggcagcgtg atgacgatcg ggcgcagcat
     6601 ctgggacgac ttcggcccca accaggactg gccgatggcg gcggcggtcg cggtgctcat
     6661 ggtggcgatg ctgatcgcgc cgatcgtgct gctgcagcgc catgggcgcg gggccgggag
     6721 ggacgaatga acggcggcgg ctggggcgga cggatgtggg tggtcggcgt gtacgccttc
     6781 ctctacgtgc ccatcctgtg cctgatcgtg ttctccttca ccgccgggga gatcaccacc
     6841 cggctcgacg gcctctcgct gcgctggtac gccgcgctgg cgcgcgacgc cgagttgcac
     6901 gccgcggtgc tgttgtcgct gcgcgtgggc gcgctcgccg ccaccgccgc ggtggtggtc
     6961 ggcaccgcgg ctgcggtgat cctggtgcgc gccgggccct atccgggcag ccgcttcttc
     7021 gtcggcatga tgagcgcgcc gatggtgatg cccgaggtgg tggtggggct gtcgctggtg
     7081 ctgctgttca cccatccggc gctcgccgtg ctgggcgggc gcggggtggc ggcgatctgg
     7141 gtggcgcaca ccacgctgtg cgcggcctac gtggcggtgc tggtggcgtc gcgcctgcgc
     7201 gagatggacc gcgcgctcga ggaggccgcg ctcgacctcg gctgtccgcc gctgaaggtc
     7261 ttcttcgtga tcaccgtgcc ggtgatcgcg cctgcgctgg cggccggctg gctgctcgcg
     7321 ttcacgctgt cgatggacga cttcgtgctc gccgcgatgc tgtccgaccc gggcagcacg
     7381 acgctgccgg tgctggtgtt cgcgcgcctg caccacggcc tcaagcccga gatcaacgcg
     7441 ctcgccacgg tgatcgtggt ggtggtgtcg atcgcggtgc tcctcgccaa ccgcctgttg
     7501 ctggcgccgc cgcgcgcgtc ggcgccagcg gcggcgcgcg gcggctgatc ggcttcagcc
     7561 ggcgaggatc tcgcgcaggg ccgccaccag aatcgcgcac tgcgcgtcgg tgccgacggt
     7621 gatgcgcatg aactggtcga tgcgcgccgc cttgaagtgg cgcacgatga tcgcgcgcct
     7681 gcgcagttcg gcggtgagtt cggcgccgtc gcgcgtcggg tggcgggcga agatgaagtt
     7741 cgccgccgag ggcagcacct cgaagccgag cgcctgcagg tcggcgacca gccgcgcgcg
     7801 cgtctcgatc acctgggcgc agctggcgcg gaagtagtcc tcgtcctcca ccgaggccac
     7861 cgcgccggcg atcgcgaggc ggtcgagcgg gtaggagttg aagctgttct tgacccgctc
     7921 cagcgcctcg atcagcgccg cgtcgcccac cgcgtagccc acgcgcaggc ccgccagcgc
     7981 gcggctcttg gagaaggtgc gcgtgaccag caggttgggg tggcgatcca ccagcgcgat
     8041 cgcgctgtcg ccgccaaagt ccacgtaggc ctcgtcgacc agcaccaccg cctcggggtt
     8101 ggcggcgacg atgcgctcga cctcggcgag cgcgagcagc cgtccggtgg gtgcgttcgg
     8161 gttggggaag atgatcgcgc cggcgcgctc gtcgccgcgc ggcaggtagt cttccacgcg
     8221 gatcgagaag tcctcggcca gcggcacgat gcgcggctgg atgtcgtaca gcccgcaata
     8281 caccggatag aagctgtagg tgatgtccgg gaaccacagc gcgcgctcgt gcttgagcag
     8341 cgccatgaag gcgtgcgcga gcacctcgtc ggagccgttg ccgacgaaga tctgctccgg
     8401 gcgcacgccg tgatggcggg cgagcgcggc cttgagcgcg tcggcgttgg ggtcggggta
     8461 gaggcgcagg ccgtcgccgg tggcggcgcg gatggcctcc agcaccttcg gcgaggggcc
     8521 gcaggggtgt tcgttggtgt tgagcttgac gaggttgtcg agcttgggct gctcgcccgg
     8581 cacgtagggg gtcaggccgt ggacgacggc gctccagaag cggctcatgc gggtgtctgc
     8641 ggttggggtg gaacggaggc ttggacgcgc acaacgcacc ttgcggcgca tcgcggcgag
     8701 ttcgctgcgc gatgttatca tgacgggcag acaacccatt ttggccggta gctcccatgc
     8761 ggcaagcaga agtcacgcgc aacaccctcg aaaccaagat caccgtgcgc atcgacctcg
     8821 atggcagcgg gcagggccgg ctcgacaccg gcgtgccctt cttcgaccac atgctcgacc
     8881 agatcgtccg ccacggcctg ttcgacctcg acatccactg cgagggcgat acccacatcg
     8941 acgaccacca caccgtagag gacgtcggca tcacgctcgg gcaggccttc gccaaggcgc
     9001 tcggcgacaa gaaggggctg cgccgctacg gccatgccta cgtgccgctc gacgaggcgc
     9061 tgtcgcgcgt ggtggtcgat ttctccggcc gcccggggct gcactacttc gtgaattaca
     9121 cccgcgcccg catcggcaac ttcgacgtgg atctggcgcg cgagttcttc cagggcttcg
     9181 tcaatcacgc cggcgtcacg gtgcacatcg acaacctgcg cggcgacaac gcccaccatc
     9241 agtgcgagac cgtgttcaag gccttcgccc gcgcgctgcg catggcggcc gagcacgacg
     9301 agcgcgccgc cgggaccatc ccctcgacca agggcgcgct ctgattcctc ccttcccgcg
     9361 gtcgcgtcgc gcggccgcca tcctgtcgaa aagcgagtca agccgatgac cagcgtggcc
     9421 atcatcgatt acggcatggg caacctgcgc tcggtcgcca aggcgatcga acacgtggcg
     9481 cccggtcacg aggtcttcgt cacctccgac ccggcgcgtg tcgccgccgc cgagcgggtc
     9541 gtgtttccgg ggcagggcgc gatgcccgac tgcatgcgcg aactcgacgc gcgcggcctg
     9601 cgacctgcgg tacgcgccgc ggcggccgaa aaacccttcc tcggcatctg catcggccag
     9661 cagatgctgt tccagcacag cgccgagggc gacgtgccgg gcttgggcat cctgtccggg
     9721 gaggtcgtgc gctttcccga gaccggcatg ttcgcggccg acggcagccg gctcaaggtg
     9781 ccacacatgg gctggaacga agtacgtcag tgcggaccgc acccgatgtg ggacggcatc
     9841 cccgacggcg agcgcttcta cttcgtgcac agctacttcg tggtgcctgc ggaggcctcg
     9901 ctgactgcgg ccgagaccga ctatggcgtt cgctttacca gtgcggtagc gcgggctaat
     9961 atcttcgcgg ctcagttcca ccccgagaag agcgccgcgg ccggcctgcg cctgctcgcc
    10021 aatttcatcc gctggcagcc ctgagcggcc ggtgattccc tctacgtttc tccactgctg
    10081 atcccggtat gctgctcatt cccgccatcg acctcaagga cggtcaatgt gttcgcctga
    10141 aacagggcga gatggacgac gccacggtgt tctcctccga tccgggcgcc atggcgcgcc
    10201 actggctcga ggccggcgcg cgccgcctgc acctggtgga cctcaacggt gcctttgccg
    10261 gcaagccgaa gaacggcgcc gcgatccgtt cgatcaccga cgtcgtcggt gacgacatcc
    10321 cggtccagct cggcggtggc atccgcgacc tcgacacgat cgagcattac ctcgacaacg
    10381 gcatcagcta cgtcatcatc ggcaccgccg cggtcaagaa ccccggcttc ctgcacgacg
    10441 cctgcagcgc cttccccggc cacatcatcg tcggcctcga cgccaaggac ggcaaggtgg
    10501 cggtggacgg ctggtccaag ctcaccggcc acgacgtggt cgatctggcg aggaagttcg
    10561 aggactacgg cgtggagtcg gtgatctaca ccgacatcgg ccgcgacggc atgctttcgg
    10621 gcgtcaatat cgaggccacc gtgcgcctgg cgcgcgcgct gcgcatcccg gtcatcgcca
    10681 gcggcggcat caccgacctg cgcgatatcg acgcgctgtg cgcggtcgag gacgagggca
    10741 tcatgggcgc gatcaccggg cgcgcgatct acgagggcac gctcgacttc gccgccgcgc
    10801 aggcgcgcgc ggacgagctc gagggcgcgc gcgaatgagc ttctcccgca ggggcagccg
    10861 atgctagcca agcgcatcat tccctgcctg gacgtgaagt ccgggcgcgt ggtcaagggg
    10921 gtcaatttcg tcgagttgcg cgacgccggc gatccggtcg agatcgcccg ccgctacgac
    10981 gagcagggcg ccgacgagat caccttcctc gacatcaccg cgagctccga cgaccgcgac
    11041 atcatcctgc acgtggtcga gcaggtcgcc gagcaggtct tcatcccgct caccgtcggt
    11101 ggtggtgtgc gcgtggtcga ggacgtgcgc cggctgctca acgccggcgc cgacaaggtc
    11161 agcatgaaca ccgcggcggt gaacaacccg cagctggtct tcgaggccgc gagcaaggtc
    11221 ggcagccagt gcatcgtggt ggcgatcgac gccaagcaga ccgcgcccgg caagtgggag
    11281 gtgttcaccc acggcgggcg caacaacacc gggctggacg ccatcgagtg ggcgcgcaag
    11341 gtcgagtcgc tgggggcggg cgagatcctg ctcaccagca tggaccgcga cggcaccaag
    11401 agcggcttcg acctcgcgct cacgcgcgcg gtgtcggatg cggtgcgcat cccggtgatc
    11461 gccagcggtg gcgtcggcac gctcgagcat ctcgccgagg gggtctccga aggccgcgcc
    11521 gacgcggtgc tggcggcgag catcttccat ttcggacagc acaccgtgcg cgaggccaag
    11581 gagttgatgc gcgcgcgcgg catcgaggta cgcttgtgag tgcgagcacg cgctggttga
    11641 acgaaatcaa gtgggacgag cacggcctgg tgccggtcat cgcgcaggaa gccgccaccg
    11701 gcgacgtgct gatgttcgcc tggatgaacc gcgaggcgct gcagcgcacg gccgagaccg
    11761 gcgaagccat ctactggtcg cgttcgcggc gcaagctgtg gcacaagggc gaggagtccg
    11821 gccacgtgca gaaggtgctg gacatgcgca tcgactgcga caacgacgtg gtcctgctca
    11881 ggatcgagca ggtgggcggg atcgcctgcc acaccggacg tcacagctgc ttcttccaga
    11941 agtattttgc cgatggccgc tgggaggccg tcgagccggt tttgaaagac ccgcaggaga
    12001 tctacaaatg atcgatatcg aagtgctgca ccgcgtgtcc gagaccctcg ccgcgcgcaa
    12061 gaaggccgat ccggattctt cctacgtctc cagcctgtac cagaagggca ccgacacgat
    12121 ctgcaagaag gtcgccgagg aggccgccga gaccatcatg gcggccaagg acaaggacat
    12181 gctgcacctg gtgtgggaag tgaccgacct ctggttccac tccatggtgc tgctcgccca
    12241 ccacgggctg tccgtggacg acgtgctcgc cgagttccgt cgccgcgagg gcgtgtcggg
    12301 catcgacgag aagaagtcgc gcactgcgga aggttcgcgc gcatgagcga ctgcgtcttc
    12361 tgccgcatcg tgcgcggcga gattccgtcg aaaaaggtct acgaggacga gcacgtcttc
    12421 gccttccacg acatccatcc cgccgcaccc gtgcatgtgc tggtggtgcc caaggtgcac
    12481 gtcgactcga tggcgcacct cgacggggaa cacgaggcgg cgatggggcg tctgatggtt
    12541 gcggcgggca ggatcgcccg cgagcagggc tgtaccgacg gcttccgcac catcgtcaat
    12601 accggaaggg tcggcctgca ggaggtgtat catctgcacc tgcacattct gggcgggccc
    12661 gatcccctgc cgcccatgtt gaagcgttaa ggagagcggt atgggttctt tcagcatctg
    12721 gcactggctg atcgtgctgg tcatcgtcct tctggtgttc ggcaccaaga agctgcgcaa
    12781 catcggttcc gacctcggtg gtgcggtgaa gggcttcaag gaaggcatga aggagggtga
    12841 ttcggccgga gcgggcgacg cctcccagca gaagctcgcc cagggccaga cgatcgaggg
    12901 cgaggcgcgc gagaaggtcg accagaaccg gtcctgagcc tcgcgtctcg aaccagcatc
    12961 gggggcgccc tgaccggagc gccccttcgt tttacggcgg gccggtggcg gcccctgtct
    13021 cgagcgagca cacatgttcg atttcggctt ttcggaactc gtggtgatcg gcatcgtgat
    13081 gctgatcgtg gtcgggccgg agcgcctgcc caaggtcgcg cgcaccgccg ggcacctgct
    13141 cgggcgcgtg caacgctacg tctccgacgt caagtccgac atccagcgcg agatgcagct
    13201 cgaggaactc aagaagctgc aggaacaggt gcggcaccag gcgcaggaac tcgagacctc
    13261 ggtgcgcacc ggtgtcgccg gggtcgagtc gggaatcggc gagacggtcg ccgaggtgcg
    13321 ctccatcctc cccgagtcgc ctgcctccgc gtcgggcacc gcggcgcccg cgccggccgg
    13381 cgctccgacg cccgtcgcga atccggcggc agcgcccgcg ccggccggag cggatgcgac
    13441 gcccgcccgt cccgccccgc aactcgagct tgggctggat agcacgcccc gcaccgcgcc
    13501 tggagctgac aaggtcaagg catgagcgaa caacaggaaa ccttcatcgc ccacctgatc
    13561 gagctgcgcg accgcctgat ccgcgcgctg ctcgcgatcg tgatcgtctt cgtctgcctg
    13621 atgccgtggg cgggcgcgat ctacgacatc ctcgccaagc cgatgatgga cacgctgcct
    13681 gcgggtacca acatgatcgc caccggcgtg gtgacgccct tcttcgtccc ggtcaaggtg
    13741 acgatgatgg tcgccttcgt gttcgcgctg ccctgggtgc tctaccaggc ctgggccttc
    13801 atcgcgcccg ggctgtacgc gcacgagaag cgcctcgccc tgccgctggt gctcggcagc
    13861 acgctgcttt tcctgctcgg catggccttc tgctatttct tcgtgttcgg cacggtgttc
    13921 aagttcatcg ccgaattcgc tcccaagagc atcgtcccgg cgccggacat cgagcagtac
    13981 ctgtccttcg tgatgtcgat gttcatcgcc ttcggcatca ccttcgaggt gccggtggcg
    14041 gtgatcctga tggtcaaggc cggcatcgtc gatgtcgcca agctgcgcga gtcgcgccct
    14101 tacgtgatcg tcggcgcctt tgtggtcgcc gccatcgtca ccccgccgga tgtggtctcg
    14161 cagttcatgc tggccgtgcc gatgtgcctg ctctacgagc ttggcatcgt gctcgcgggc
    14221 atgctgtcca ggccttcccc tcagcaggag gccgccggcg agacggtaca gcagctcgcc
    14281 gggccggctg ctgcggatgg cgagggcgag ccccgctgag tgccgcgggc cgcgtcggtc
    14341 ggtgcctcag cgcgcggcgg gcgggatcgg gcggcggccc acttccacct cgaggtcgag
    14401 cgcctcggcg ccgcgccgca ccctgaacac cgcacgcttg ccgggtgaga gcgccgcgac
    14461 catgtcgagc atcgcgcgcg ggtcgcgcac gctctcgccg tcgagcgcga ccaggacgtc
    14521 gcccggacgc acgcccgccc gatctgccgg gctgccgcgc agtacgccgg cgatgagcgc
    14581 gccgccggcg tcgcgatagc cgaacgaggc ggccagttcg ggtgtgaggt cctggatctc
    14641 cacgcccacc cagccgcgca cgacctcgcc ggtggcgacg atctgctcga gcacgtcgcg
    14701 cgcgatcgag accgggattg cgaagccgat gccgagcgaa ccgcccgagc gcgagtagat
    14761 cgccgtgttg atgccgacca ggctgcccgc gctgtcgacc agcgcgccac ccgagttgcc
    14821 cgggttgatc gcggcgtcgg tctggatgta gttctcgaag gtgttgatgc cgagctggct
    14881 gcgaccgagc gccgagacga tgcccatggt gaccgtctgg ccgacgccga aggggttgcc
    14941 gatcgccagc accacgtcgc ccaccgcgag gctgtcggcg gccgggaagg tgatcgccgg
    15001 aagcgcctcg gcgccgtcga tgcgcagcac cgcgaggtcg gtctcggggt cgcgcccgac
    15061 caggcgggcg gcgaacttgc ggccgtcgtt gagcgcgacc tcgatcgcgt cggcggtctc
    15121 gatgacgtgg ttgttggtga gtacgtagcc gtcggggctg acgatgacgc ccgagccgag
    15181 gccggattcg ctcggtgcgc tgtccgggcg ctccccgaag aagtggcgga acagcgggtc
    15241 gtcgagcagc gggtggcgct ggccgcgacc gcccttgctg gtgaagatgt gcactaccgc
    15301 cggcatcgaa cgctgtgcag ccggggcgta ggagcctgcc gccggcgcac gctcgctgcc
    15361 cgcaggtgcc tcgaggatcg ccaccaccgc cgcgggcgtg gccttgcgca gccattccgg
    15421 cttgagggtg ttgagcacga acagcacggc gacgctgacg gtcaccgcct gcgcgaagat
    15481 gagccacaag cggcgcataa tgtcgggctt caaagaagag ggggtcgcgc ccggccggcc
    15541 ggagcgttct ccggtcgagg agccacgcgc gaagcaagga tgaaggagga cgcatgcagc
    15601 tcaccgaact cgggggtcat ctcgacaccc tgctcgacgc ttcacggctg aaggattatt
    15661 gcccaaacgg cctgcaagtg gaaggacgcg cggaggtccg gcgcgtgctg tgcggggtga
    15721 ctgccagcca ggccctgctc gaccttgcag caacgggggc gtatgacgcc gtcttcgttc
    15781 accatggcct gttctggaag ggcgacgacg gtcgcgtgac cggcttccgg cgtcggcgcc
    15841 tgcataccct gctcgccaac gacatcagcc tgttcgccta ccatctgccg ctcgacgtgc
    15901 atccggagct cggcaacaac gcgcaactcg cgcgcctgat gggctggcag gggacggcgc
    15961 gcttcgccga ccaggagctg ggctggatcg gccggccggc gcgggagggc gagcgtgcgg
    16021 acgagatcgc gcgcgcgctt gcccggcggc tcgggcgcga gccgctgctg gtcggtgacg
    16081 gcgagcgtac ggtgcgccgg atcgcctggt gcaccggcgg tgcgcagggc tttttcgagc
    16141 aggcgatcct ggccggggcc gagctctacg tgtcgggcga gatctccgag cccacggtgc
    16201 atctggcgcg cgagtccggc gtgccctaca tcgccgccgg ccaccacgcc accgagcgct
    16261 acggcgcacg cgccatggcg cactacctga acgaggtgct cgggctggag gcgagcttcg
    16321 tcgacctcga caacccggtc tagcgccggg ggggctcgtc cgcggccggc gggcaggggc
    16381 cgaggtcttc ctcgaggatg gcggtgaggg tgaggatctc gtcgatcacc gagaacggga
    16441 agccggcgcg ggtgccgttg cggttgtcgc gcagcagctg ctgcaggtgg cgctggcagg
    16501 cggcggtgcg ccatgtcttc tgcagggcct cgcagatgtg cggcaggtcc tccaggctca
    16561 tcttcgacgc ccgcagcgcg tcgtagctct gccagttgac cgtggtgacg ttgaaggtct
    16621 tgttgagctc cccggcgatc gcgttgaact cgtcgcgcag gcccgctgcc cggtagacct
    16681 cgagcagctt gagccagggc gagaccgctt cgcgcggatt gccgcggatg aactcggcga
    16741 gcgtctcggc tgcgccgcgg acgcggccga agctcatcat gatgtcggcg agctcgaccg
    16801 cggacttgtg ctcctggatg gtctcggttg ccgtccccgc cggccactcg aggcgagtgt
    16861 cgacggtgcc gacgccgaag cctaagccgg tctcttcggc ccgcgcgcgg cccttgcggg
    16921 actccgcgct gccgagcgtt gttgcggtgg cggtgtccgt cggccgactg gacggcgctg
    16981 ccggcgtcgg acgccgcgag cggcgcaaca gcgcgacgag ggccaaggcg gcaaacccga
    17041 gtccgccgaa caggtaggcg tcgcggcccc aggcgttctc ggccgcgagc gcagggcgct
    17101 tttcggcggg ggtgaccggc tcggtccgcg gcggaggcga ggcggggact gcgacggccg
    17161 gggcggttgc gacggcgggg acggcctgca tcctgagcag gcgctccttc agcccggcct
    17221 ggatttcctc gagctccttg atgcgggcga gcagctccat ctcggcgatg atgctgcggt
    17281 cgatcgccgc ggcaagcgcg cgctcgcgct ccgcgtgatt gcgcgggggg gcgtcgccat
    17341 gcgccgccgt ggtcccgccc gtgctccgcg tggcgacggt cttgtccacg acgagccggt
    17401 cgggtccctg ggcgacaacc ggtgccggtg ccgcttcggg ctgcggcgcg gcggggcgca
    17461 ggtcgggcag gcgcagcgcc gtgcctgcgg gcaggacgcg ggtgagtgcg gcggcgtcgg
    17521 cgaacaggtt cgggtttgcc gcggcggtcg cctcgatgaa gcgcgcctgc gcggcggggt
    17581 cgtccgggta cagcgacgac gccagggtga cgagcgattc gtcgccgctc gtgccgaggg
    17641 tgcgcgcgcg tggtttcggc gccgccttcg cctgcgcggg cggtgccgtg cgcggtgtcc
    17701 gcgcgggcgc gcgggcggcc gggggtggcg cggcggctgc caccgcagcc gccggcaggc
    17761 gggcgccggg tgcgaagggc atcagcagcg tgtactcgcg ccgcaggcgc gcctcgcaca
    17821 cgttctcgat cgccagcttc agcaccggct cgttgacgcg ggcggcgtcc cgcaccacca
    17881 ggcgcgcttc ggcaccgctg ccgctgacgg cgatacggcc ctggcgcaag gccggaaact
    17941 ctccaccgga gaggggggcg acgcgaaagc attcggcatc ggcaggtttt ccgtccggca
    18001 ggcggatctc cgcgcggaag ctctccccca tcgcggagag tgcggcgacc tcgccgagcg
    18061 ccaccgccgc cgcgggaggg gcggtgccga gcgaggcgag ggcgattgcg actgcgattg
    18121 cgagcggcat gctgttgttg ttcatattcc gcgcgtccgg cggtggatcg aatgattgcc
    18181 gagttttagc agactcgccg taggcattcc agcgactcgt gccgcaatgc gaccgtgttt
    18241 tgttgcaggt gtgacgtatt cagcgttccc ggatgcttcc gccacacggc agccggcgcg
    18301 gtgcgggatg cgggtggagc gggcccggtg cggctgccgg cgcatggctg tgcaccgcgc
    18361 aaaaccagtt ataattatgg ggtcttgcga ccgcggcgtg cgcgcccggt ccgcgggtcg
    18421 ggtcgggggg tcccgattcc agtgcggcgg gcgacggtcg agggagacgg cgccggtcgt
    18481 ggagcgatcg ctccgaagca tgccatcaac ctgtccgcgg cggcggctgc cgtcgcggct
    18541 tcgatcgaag ggcaaccatg aaaattcatg agtatcaggc aaaagaactg ttgaggaagt
    18601 acggcgtcgt cacgccgcgc ggtttccact gcgtatcggt ggacgctgcc gtgaaggcgg
    18661 ccgaggaact cggcggcaag atctgggtgg tcaaggccca gatccacgcc ggcggccgcg
    18721 gcaagggcgg cggcgtcaag ctcgcccgct cgctcgacga agtgcgccag cacgccaacg
    18781 acatcctcgg catgcagctc gtgacccacc agaccggccc cgaaggccaa aaggtgcgca
    18841 acctgctgat cgaggaaggc gccgacatca agcacgaata ctacgtcgcg gcgctgaccg
    18901 atcgtgccac ccagacggtc gccatgatgg cctcctccga aggcggcatg gacatcgagg
    18961 aagtcgccca caacaccccc gagaagatca tcaaggtctt cgtcgatccg ctcgtcggcc
    19021 tcaccgacgc gcaggccgcc gagcttgcca agggcatcgg cgtgccggaa ggctcggtcg
    19081 ccaaggcggt cgacgccttc aagaagctct acacctgcta catggaaacc gatgcctcgc
    19141 tggcggaaat caacccgctg atcctcgagg gcaacggcaa catcaaggcg ctcgacgcga
    19201 agttcaactt cgactccaac gccctctacc gtcacccgga gatcgtcgat ttccgcgact
    19261 tcgacgaaga ggatgccgac gagatcgaag cctccaagta cgacctcgcc tacatcagcc
    19321 tcgacggcaa catcggctgc ctggtgaacg gcgccggcct ggcgatggcc accatggaca
    19381 cgatcaagct gttcggcgcc gagccggcca acttcctcga cgtcggcggc ggcgcgacca
    19441 ccgagaaggt caccgaagct ttcaagatca tgctcaagaa cccccaggtc aagggcatcc
    19501 tggtcaacat cttcggcggc atcatgcgct gcgacaccat cgccaccggc gtggtcgccg
    19561 cggcccgcga agtgaacctc tccgtccccc tcgtggtgcg catgaagggc accaacgaag
    19621 acctcggcaa gaagatcctc gccgattcgg gcctgccgat catcgccgcc gacacgatgg
    19681 cggaagccgc gaccaagatc gtcgctgcgg tcaagtaagg agcacttcga atgtccatcc
    19741 tgatcaacaa agacaccaag gtcatcaccc agggcattac cggcaagacc ggccagttcc
    19801 acaccgagaa gtgcatcgag tacgccaacg gcaagaactg cttcgtcgcc ggcgtgaacc
    19861 cgaagaaggc cggcgagaag atcctcgacg tgccgatcta cggctcggtc aaggatgcca
    19921 aggacgccac cggcgccacc gtctcggtga tctatgtgcc gcccgccggc gcggccgccg
    19981 cgatctggga ggcctgcgag gccgacctcg acctggcgat ctgcatcacc gagggcatcc
    20041 cggtccgcga catgctggtc gtgcgcaaca ggatgaagca gaaggtcgcc gccggcggca
    20101 aggaaaccct gctgctcggc cccaactgcc ccggcctgat cacgcccgac gagatcaaga
    20161 tcggcatcat gcccggccac atccaccgca agggccgcat cggcgtggtc tcgcgctccg
    20221 gcacgctgac ttatgaagca gtcgcccagc tcaccgagat cggtctcggt cagtcctcgg
    20281 cggtcggcat cggcggcgac ccgatcaacg gcctcaagca catcgacgtg atgcgcatgt
    20341 tcaacgacga tccggacacc gacgcggtga tcatgatcgg cgagatcggc ggtccggacg
    20401 aagccgaagc cgcgcagtgg tgcaaggcca acatgaagaa gccgatcgtc ggcttcatcg
    20461 ccggcgtcac cgcgccggcc ggcaagcgca tgggccacgc cggcgcgctg atctccggcg
    20521 gcgccgacac cgccgacgcc aagctcgcca tcatggaaga gtgcggcttc aaggtcacgc
    20581 gcaacccgtc cgagatgggc aagctgctca aggcgatgct gtaagccgtc ccccggggca
    20641 tggcaagggg cgccggattt tccggcgccc ttttttgtta accggcgcgg tgcgcgcgct
    20701 agaatgggcg gaggattcct cgcaggcggc gcaggcgcgc cggccgttcc acttacagac
    20761 gggtatgggc atgccgaagt tggttctcag catggacggg ctggtgctca aggaaatggc
    20821 gctcgtcaag gagcgcacca ccatcgggcg caagccggac aacgacatcc agatcgataa
    20881 tctcgcgatc agcggccatc acgcggtgat cacctgcatc accaacgacg ccttcctcga
    20941 ggaccagaac agcaccaacg gcacctacct caacggccag ccggtcaaga agaacgtgct
    21001 gcgcaacaac gacgtcatcg agctcggcaa gtaccgcatc aagtacatcg tggacgatgc
    21061 caagcccggg ctggcgccgt ccgagatgat cgagaccgcg gcctacaagg ccttcgagat
    21121 gccgcgcgcc acgaccgaga cggtgcttcc gccgggggcg ccggcgccga gcgacacgca
    21181 gatcctctcc gccgccgcgc gccaggccgg caccgcggcc gcggccgcca ccgccgcctc
    21241 cgcggcgggc gaagggcagg gtgtgatcca ggtgctcagt ggcgccaacg ccgggcgcga
    21301 gctgccgctc accaagtcgc tcaccacgct cggcaagccg gggcgccagg tcgcggtgat
    21361 cacgcgccgc ccgcacggct acttcatcac ccacgtcgag ggggcgagtt ttccgctcgt
    21421 caacggcagg gcgatcgacg cccaggcgcg tcccctgcgc gaccacgaca tcatcgagat
    21481 cgcgggcgtc aagatggagt tcttcctgcg cgcctgaggg gccggggagg gcgtgacatt
    21541 ctcccgcgcg gccggcgcgc ggtcggtgct aaggtttcgc atgggtttgc acggtggcct
    21601 ccggccgccg cggacgcgga tgacggaggc catgaagctc aaggtgctgg ggtgcagcgg
    21661 cggaatcggc ggggcgcaac cgcgcaccac ctcgttcctg ctcgacgacg acatcctgat
    21721 cgactgcggg acgggcgtcg gcgacctgac gctggacgag ctggcgcgga tcgaccacgt
    21781 cttcctcacc catgcgcacc tcgaccacat cgccgcgctg ccgctgatga tcgactcggt
    21841 gggcgaactg cgccaggcgc cggtgcaggt gcatgcgacg cccgagacca tccgcatcct
    21901 gcactcgcac atcttcaact ggctggtgtg gcccgatttt tccgcgattc ccgaccacca
    21961 ccagcccttc atgcgcttcc atccgatcca cgtcggcgag cgccgcgatt gcggcgggcg
    22021 cggcatcacc gcgctgcctg cccgccatac cgtccccgcg gtggcctacc ggctcgacag
    22081 tgcgcacggg cagctggtgt tttccggaga taccgcctac tgtcccgagc tgatcgaggc
    22141 gatcaacgac tgtccgcaac tgcgccacct catcctcgag accgccttcg ccgaggaaca
    22201 gcacgggctc gccgtggctt cgcgccacct ctgcccgagc atggcgcgcg cggtgctcga
    22261 cgagatccgc ggcacgcccg agatccacat cagccacctc aagccgggcg tcgggcagcg
    22321 catcatcgac cagctcgggg cctgcgcgcc cggacgaccg ctgcgcgcac tcggtcgcgg
    22381 cgacctcctg gagttctgat gcgggtaccg ggcgcggcgc ggaatccgct accttgcatg
    22441 ccaccgctcg cccccgggcg tctccatccc gaggaatcct catgaacacc tccgtgtcca
    22501 gcgccgtcac gcaccgtctc gccttcttca agggcttgca gacgctgacc aaccgcatcc
    22561 atgcgagcaa ggacaccgac gagatcatgc tcgagctcgc gcccgagctg tgctcgctgt
    22621 tcgaggccga gcgcttcacc atctacgcgc tggaggaggg cggggcctcg atcgtcaccc
    22681 gggtcaagac cgggctgagc ggggtgcaga gcatccgcct gccgatcggc gagaacagca
    22741 tcgccggctt cgtcgcgctc agcggccaac tggtcaatat ccgcgacgtg tacgacgacg
    22801 aggagctgcg ccgcatctcg ccgcacctcg cgctgcgccg cgaggtcgac gccagcaccg
    22861 gcttccactc gcgccagatg ctggtcgcgc ccatccgcga tcccgacacc ggcagcctgc
    22921 tcggggtcgt gcagctcatc aacacgctca accgcgaacc cttctccaag atgaccgagg
    22981 aggggctgat ggggctcgca cagacgctcg gcgtcgcctt cctgcgccac aaccgccagg
    23041 ccgggcgcct gcgttcgcgc ttcgaggcgc tggtggtcga tggccgcatc accgccgagg
    23101 agttcgccga cgccacccgc gaggcgcgcg agagcggggc gggcaccgag aacctgctga
    23161 tcgagcagct cggcctcagc cacgccgaac tcggcgcagc ggcctcgcgc tatttcggcg
    23221 tgccctatca gcccttcgtc gccaaccacg tcaagccgat ggacctgctg cgcaacatca
    23281 agcgcgagtt cgtcgagcgc agcctgtggc tgccctggga ggagaacccc gagggcgtgg
    23341 tgatcctgtg cgtcgacccc gagcaggtgc gcagctcggg cgtggcgcag aacgtgctgc
    23401 cgaagaagcg gctgacctac cgcgtcacca cccagcgcga cttcgagctg acggtcgagc
    23461 agttcttcgg tgtgaccctg gacgacgcct cggtctcgga cctgctgtcc ggtctcgacg
    23521 atcacgagga ggacgccctc tcgctccggg acgaggtcaa cgccgccgcc gacaacgagc
    23581 tcgtcaagct ggtcaacaag atcatcatcg acgcctatcg tcagggcgcc tccgacatcc
    23641 acatcgagcc ccgcccgggc aaggagaaga cccagatccg cttccgcaag gacggctcgc
    23701 tggtgcccta catcgagatc ccggcaagct accgcaaccc gctcgtcacc cgcatcaaga
    23761 tcatgtgcga cctcgacatc tccgagcgcc gcaagccgca ggacggcaag atccgcttcc
    23821 gcaagttcgc cccggtcgac ctggagctgc gcgtggccac gctgcccacc cagggcggca
    23881 tggaggacgt ggtgatgcgc ctgctcgcgc acagcgagcc gatcccgatc gaccagctcg
    23941 gcctgctgcc cttcaatctc gagcgcctca agagcgcgat cgccaagccc tacggcatct
    24001 tcttcgtgtg cggccccacc ggctcgggca agaccaccac gctgcactcc atcctgagct
    24061 acatcaacac cccggatacc aagatctgga cggtcgagga cccggtcgag atcacccaga
    24121 agggcctgcg ccaggtgcag gtcaaccgca aggccggcat cgacttcgcc accatgatgc
    24181 gcgccttcct gcgcgccgac ccggacgtga tcatggtcgg cgagatgcgc gaccacgaga
    24241 ccgtctcggt cggcatcgag gcctcgctga ccggccacct ggtgttctcg accctgcaca
    24301 ccaacagcgc gcccgaatcg gtcgtgcgcc tgctcgacat gggcatggac ccgttcaact
    24361 tcgccgacgc gctgctcggc gtgctcgccc agcgcctggc caagcgcctg tgcctgaagt
    24421 gccgggtacc ctaccgcgtc ggcgaggacg agctcgccag cctgctcgac gaatattgcg
    24481 aggacatgca cctgaccccg gacttcgccg ccgacccgaa gaccgcccgc gagcgcgtgc
    24541 tggggcgctg gcgcgaggcc ttcggcgaca aggacggcgg cttcaggctc taccgcgcgg
    24601 tcggctgcga gcactgcaac cagggctaca aggggcgggt aggcctgcac gagctgatgc
    24661 tcggctccga cgcgatcaag cgccagatcc agtcgcgcgg ccgcgtcgac cagatcctcg
    24721 gccaggcgct cgccgaaggc atgcgcacgc tgcgccagga cggcatcgag aaggtgctcg
    24781 ccggcatcac cgacatgaag caggtgcgca aggtctgcgt gcgctgagcg cggcaggggc
    24841 ggccgatcgg cggggcgcgg gttagaatcc gcgctgccac ccgcaccgga gcccaccatg
    24901 taccgcatcg cccccagcct gctgtccgcc gacttcgccc gcctcggcga ggaggtccgc
    24961 gacgtcatcg ccgccggcgc cgactggatt cacttcgacg tgatggacaa ccactacgtc
    25021 cccaacctca ccatcggccc gctggtgtgc gaggccatcc gcccgcacac ctcggcgccg
    25081 atcgacgtgc acctgatggt gaagccggtc gatcgcatcg tgcccgactt cgccaaggcc
    25141 ggcgccaacg tcatcacctt ccaccccgag gcctccgagc acgtcgaccg cacgctcggg
    25201 ctgatccgcg acgccggctg tcaggccggc ctggtgttca acccggccac accgctcgac
    25261 tggatggacc acgtcatgga caagctcgac gtggtgctgc tgatgagcgt caaccccggc
    25321 ttcggcgggc agaagttcat ccccgagacc ctcaacaagc tgcgcgccgc gcgtgccagg
    25381 ctggacgcct acgaggcgcg cagcggtcgc cgcatcctgc tcgagatcga cggcggggtg
    25441 aagaccgaca acatcgccgc gattgcccgc gccggagccg acaccttcgt cgccggctcc
    25501 gcggtattcg gtgccgcgaa ggccgccgat ccgcaccgct acgactcgat cctgggtgcg
    25561 ctgcgcgccg agctcgccgc ggtgcaggca tgaggatgga cgcgcgcttc gcgccgcgcg
    25621 cggtgctgtt cgacctcgac ggcaccctgc tcgacaccat cgccgacctc gccgacgccg
    25681 ccaacctcat gctcgccgag ctcgcccgcc cggcgcgcag cctggaggag atccacagct
    25741 tcgtcggcaa gggcattccc aacctggtgc ggcgctgcct caccgagggc acgcacgccg
    25801 atgaggacga gatcgactcc gccgtggccg tgttccgccg ccactatgct gtggtcaacg
    25861 gcgcgcgcac gcgcatctac cccggggtcg gcgaactgct cgagcgcctg cgcggacacg
    25921 ggctgccgct ggcggtggtc accaacaagg ccgaggcctt cacgctgccg ttgctcgagc
    25981 gcatggcgat tgcgcactat ttcgacgccg tggtcagcgg cgacaccttg ccggtcaaga
    26041 aacccgaccc ggcgatgctg cacctggcct gcgcgcgcct cggcgtggcc gcccggcagg
    26101 cgctgatggt cggcgactcg gccaacgacg ccttcgccgc gcgcgacgcg ggcatgccgg
    26161 tgctgctggt gcgttacggc tacagcgaag gcatgccggt ggacagcatc gaatgcgatg
    26221 ggctactatc gaacgcactc gaagtgctgg accatatcgg tcctgcatag gcgcaaaccg
    26281 gcggcagggg cggcgcaatc cctcgcaatc ccgctgcggg ctcgcaaggt ccggtggctg
    26341 cgccggcgcc ctttcatccg ccctcgtccg tctctccacc aacgacttca tcgtgatgct
    26401 cgcaccccgg gatcgcctgt tctccgcctc gtcctccgcc cagcccgact ggcgcggcgg
    26461 ctggcgttgg cctttgggcc actgaacgcg acctccccct gcggggcccg ccgcgcacgc
    26521 tgcgcgacgc cttccccgcc cgctgcaagc ccttccgatc gatccgtccc acgtggagcc
    26581 cccatgctcg aacacgaatt caacgccctc gccgccgagg gctacaaccg catcccgctc
    26641 accctcgaga ccttcgccga cctcgacacc ccgctctcca tctacctgaa gctcgccaac
    26701 gagccctaca cctacctgct cgaatcggtg cagggcggcg agcgcttcgg gcgctattcc
    26761 ttcatcggcc tgtcctcgcc cacccgcatc gaggtctatg gccgctccgc cctgctgctc
    26821 accggcaacc gcctggtcga gcgtcgcgac tacggcgacc cgctcaactt cgtcgccgag
    26881 ttcatgaacc gcatcaaggt gccgccgcgc cagcacctgc cgcgcttcgc cggcggtctg
    26941 gtgggctgtt tcggctacga caccgtgcgc tacatcgagc cgcgcctggc caagaccgag
    27001 aaagccgaca ccgtcggcac gcccgacatc ctgctgctgc tgtcggaaga agtcgcgatc
    27061 gtcgacaacc tcagtggcaa gctcaccctc gtcgtctatg ccgagcccga ggtgccgggc
    27121 gcgtacaagc gcgcgcagag gcgcctgcgc gagctcctcg cccgcctgcg cgaggcggtg
    27181 gagatccccg aggagttccg cggcgagtcc gccgagccgg tgtcgagctt cggcgaggaa
    27241 gccttcaagg acgcggtgcg ccgcgccaag cagtacatcg tcgacggcga cctcatgcag
    27301 gtcgtgctgt cgcagcgcat gagcaagccc tacgccgcca gcccgatggc gctgtaccgc
    27361 gcgatccgct cgctcaaccc ctcgccctac ctgttctact tcaacctcga ggacttccac
    27421 gtcgtcggcg cctcgcccga gatcctcacc cggctcgagg acgacgtcgt caccgtgcgt
    27481 ccgatcgccg gcacccgcaa gcgcggcgcc accccggccg aggaccttgc gctcgaacag
    27541 gaactgctcg ccgatcagaa ggagatcgcc gagcacgtgc aactgctcga cctcggccgc
    27601 aacgacgccg gccgcgtctc cgaggtcggc acggtcaggg tcaccgagcg cttcaccgtc
    27661 gagcgctact cgcacgtgat gcacatcgtc tccaacgtcg aaggcaagct gcgcgagggc
    27721 ctcgacgcgc tcgccgtgct gcgcgccacc ttccccgcgg gcaccgtgtc gggcgcgccc
    27781 aaggtgcgcg cgatggagat catcgacgag ctcgagccgg tcaagcgtgg catctacgcc
    27841 ggcgcggtgg gctacctcgg cttccatggc gacatggacc tggcgatcgc gatccgtacc
    27901 gcggtggtga aggacggcca gatccacgtc caggccggcg ccggcatcgt cgccgactcc
    27961 gaccccgacg ccgagtggaa cgagacccag agcaaggcgc gcgcgatgct gcgcgccgcc
    28021 gagatggccg aaggcggact cgacacccgc ttctgagcgc ggcgcctgcg ccgccctccg
    28081 tccaccacgc ctcgatactg gcgatgcccg cgcggcgcgc ggcttcgcga agggaaagac
    28141 catgctgctg atgatcgaca actacgacag cttcacctac aacctcgtgc agtacttcgg
    28201 cgaactcggc gccaaggtca aggtgtatcg caacgacgag atcaccctcg agcagatcgg
    28261 ccgcctcgcg cccgaacatc tcgtcatctc gcccgggccg tgctccccgg cccaggccgg
    28321 catctcggtg gcggcgatca aggccttcgc cggcaagctc cccatcctgg gcgtctgcct
    28381 cggccaccag agcatcggcg aggccttcgg cgggcgcgtc gtgcacgcga agcgcctcat
    28441 gcacggcaag acctcgccgg tgcatcacat cgaccagggc gtcttccgcg gcctgcccaa
    28501 cccgctcacc tgcacgcgct accattcgct ggcgatcgaa cgcgccagcc tgcccgattg
    28561 tctggaagtg acggcgtgga ccgaggacgg cgagatcatg ggcgtgcgcc acaggacgct
    28621 cgacgtcgag ggcgtgcagt tccaccccga gtccatcctc accgagtgcg gccacgcgct
    28681 gctgaagaac ttcctcgacc gcggcgccag ccagccggcg gccgcctgaa acgaacgctg
    28741 cgagagcccc atgaccacga ttaccgccca gcaagccctg caacgcacga tcgaacaccg
    28801 cgagatcttc ttcgacgaga tgctctcgct gatgcgccag atcatggccg gcgagatctc
    28861 cccggtgatg accgccgcca tcctcaccgg cttgcgcgtc aagaaggaga ccatcggcga
    28921 gatcaccgcc gccgccacgg tgatgcgaga gatgtccacc aaggtcgtgg tcgagccgcc
    28981 gcacgaccac ttcctcgacg tcgtcggcac cggtggcgac ggcgccaaca ccttcaacat
    29041 ctccaccacc acgatcttcg tcgccgcggc cgccggcgcg cgcgtcgcca agcatggcgg
    29101 acgcggggtg tcgtccaagt cgggcgcggc cgacgtgatc gaggcgctcg gcgtcaacct
    29161 ggatctcacc ccgcagcagg tcgccgagtg catcgaggcc accggcatcg gcttcatgta
    29221 cgcgcccgcc caccacagcg cgatgaagaa cgtcgccccg gtgcggcgcg agatgggtgt
    29281 gcgcaccatc ttcaacatcc tcggcccgct caccaacccg gccggcgcgc ccaacacgct
    29341 gatgggcgtg ttccatcccg acctggtcgg catccaggcg cgcgtgatgc agcgcctcgg
    29401 cgccaaccac gtgctggtgg tgcatggcct ggacggcatg gacgaggtca gcctgggcgg
    29461 tgccaccctg gtcggcgagc tcaaggacaa caaggtcgtc gagtacgaga tccaccccga
    29521 ggacttcggc ctcgccatgg cgggcagccg ccagctgcgc gtcgaggacg ccgccgagtc
    29581 gcgcgaggtg ctgcttggcg tgctcgacaa ccgccccggc ccggcgcgcg agatcgtcgc
    29641 cttcaacgcc ggcgtggcgc tgtacacggc caacctggtc gacaccatcg gcgccggcat
    29701 cctgcgtgcg cgcgagatca tcgagagcgg cgccgcgcgc gccaagctcg acgagttcgt
    29761 gcgtttcacc cggcagttcg ggagcggccg atgagcgaca tcctgcagaa gatctgcgcg
    29821 gtcaaggtcg aggaggtcgc cgccgccgcc gccgcgcggc cgcagtgtct ggtgcgcgag
    29881 gacgccgcgg cgcagccgcc cgcgcgcgat ttcgtcggcg cgatccgcgc caagcacgcc
    29941 gcccgccgtc ccgcggtgat cgccgagatc aagaaggcca gcccctccaa gggcgtgatc
    30001 cggcccgact tccaccccgc cgagatcgcc gccgactacg agcgcgccgg cgccgcctgc
    30061 ctgtcggtgc tcaccgatcg cggctttttc cagggcgcgc ccgaatacct ccaggctgca
    30121 cgcgccgcct gccacctgcc ggtgctgcgc aaggacttcc tggtcgatcc ctaccaggtc
    30181 ttcgaagcgc gcgcgatggg ggccgacgcc atcctgctga tcgccgcctg cctcgacctg
    30241 gcgcggatgc gcgacatgga gcagctcgcg gtcgagctcg gtatggcagt gctggtcgag
    30301 gtgcatgacg ccgcagagct cgagcacgcg ctgcagctcg agaccccgct gatcggcatc
    30361 aacaaccgca acctgcgcac cttcgaggtc tcgctgcaga ccacgctcga cctgttgccg
    30421 cgcatcgcca gcggcgcggc gggccgcatc gtggtcaccg agtcgggcat cctgaagccc
    30481 gaggacgtcg ccctgatgca ggccaatgcg gtgcacacct tcctggtcgg cgaggccttc
    30541 atgcgtgcgc cgcatccggg cgccgagctg caggcactgt tcggccgctg aggcgcccgg
    30601 gtgagcgcgc acgaccacga cgcctcgccc gcgcccgccg cgccgccacg ccggcgccgg
    30661 ctggcgcgcg cgctcggctg gggcgtcgcg ctgccgctgg cgctgctgtg cggcggcggc
    30721 gcctggctcg ccggcacgca atccggcctc gacgccgcgc tcggcctcgc cgcacgggcc
    30781 acttccggcg cgctgcaggt cgagggcgcg cgcggccggc tgttcggccg tctcgacgtg
    30841 gatcggctgg cctggcgcgg gccggggctg gagctcgaag tgcgcgcgct ctcgctcgcg
    30901 tggacgcccg cggcgctgct gcagcgccgc gtcgatatcg agcatctgac cgcgggtcgc
    30961 gtgctgctcg cccaggcggt ggccgaggac ccgcccgcct ctgcgcccct ggcggcgcca
    31021 tcctccttgg cgctgccggt cgcgatcgcg ctgcgccggc tcgccgtcgg cgccttcctg
    31081 atgcgcgagc tgcccgcagg cggtgacctt gccgtgccgc tcgccggcgc cgcaccgggt
    31141 tcggcggatg ctgccgtgcc ggtgcagggc gaggtcgcgg ctgcggcggg cgaggcggcg
    31201 caggcgctcg atcgcgccgc cgacgtcgcc agcgacccgg cgctcgccgc cgaggccgcg
    31261 accgaggtcg ccgccgagca gggcctggtg ttctcccgcc tggaggccgc gctctccagc
    31321 gacggccgcc atcaccgcct cgaatccctc tccgtcgcgc tgccgcaggg ccgcgcccgc
    31381 gcggcgctcg cgctcgacgg cgtggcgccc ttcgcgctcg cgggcgaggc gggcttcgac
    31441 ggcgtggtcg agacccacgc cgtcgcggtg caggcgacgc tggccgacag cctgctcgaa
    31501 ccgcgcctgc aggccaaggc cacgctgggt gcggcgcatg cccagatcga ggcccgggtc
    31561 gagagcttcg ccgccgatcc cctgcggcaa ctgcggctga aggccggcgg catcgatccc
    31621 gcggccttcg ccgccggtgc gcccagcggc gagttcgcca tcgaggccga gctcgccgcc
    31681 cccgccgcgg gcgaaggcct gctcgccggc ccgatccgcg tcaccaacgc ccgcccggcc
    31741 gcggtggacg ccggcgggat tccgctcgag gcgctgtcgg cgcaactcga ctggaagcgc
    31801 gccgagctcg ccctcgacgc cgtcgagttc cgcctcgccg gcggcggcac catcgccggt
    31861 cgcgtggcct ggcacccgcc tgcgctgccc gaagcgaccg cctccgccgc gccagcggcc
    31921 gaggccgggt tcggccagtt cgtcgctgcg ctcgaactcg ccggcatcga tacgcgcgcg
    31981 ctcgacggcc gcctgccgcg tcagatcgtc gccggccggg tggatgccga ggccggcgcg
    32041 cagcgccaga cggcgcggct ggccctcatg gtgggcgcgg cgcgcatcga cgccgacgcc
    32101 agcctgagcg tcgtgcccgc cgcgcccccc tcgccggtgg ccgctgcgag cgccgccggc
    32161 gagggggggg cgcaaaccgc tcgtgaaggc gccgctgccc cccctgttcc tgcgccggca
    32221 acggacctgc ccgccgccgt gcacgccgga gcggcacgca gcttcaccct ctcggcccgc
    32281 ctgcgcggcg tcgatccgcg cgcgctgcgg tccgagctgc cgagcgctcg cctcgacctc
    32341 gatctggacg cgcgcggcga cctgtctgcc gaggggctgc cgcaggcggt cgcgctcgac
    32401 ttcaccctcc ccgacagccg cctcgacggg cgcccgctgc gcggcgaggg ccgcctgcgt
    32461 gtcgaaggcc tgcgcgtgcc cgaggcgaag atcgacctga acatcgccgg caacaccctg
    32521 cgcgccgagg gggctttcgg cgcgcccggc gacagcctgg cgctgcgcct ggacgcgccc
    32581 gcgctggcgg cgatcggctt cgggctcggc gggcgcgccg gcgccgaggg ccgcatcggt
    32641 ggcacgctgg aagcgcccaa cgggcgcctg gacctcttcg gcgagaacct cgccctgccc
    32701 ggcgacgtgc gcctggcggg cttgaacgcc cgtgtccgcc tcgattccgg catcgacggc
    32761 cccttcgagc tcgcgctcgg cctcagccgg ctcggcagcc gcgcgcgcgg cgccgacggc
    32821 gcgccaccgc ccgactggct ggcgagtgcg cgcatcgagg ccggcggcac gcgcgcgcgc
    32881 caccgcctcg acgccgacct cgccacaccg aaagtggagg gcgtcgccga caccctgcgc
    32941 ctcgcgctcg aaggcggcct gcctgcgggc gaggcgccgc gctggagcgg ccgcctcgcc
    33001 gcgctcgagg tcggcggacg tttccccgcc cgtctgcagg cgccggccac gctcgagctc
    33061 ggcgccgatc gcgtgcgcct cggtcgcgtc gagatcgagg ccggcgacaa gggccgcatc
    33121 cgcctgctcg agaccgcctg gtcgccgacg cagagcgtgc tgcgcggcga cctcgccggg
    33181 ctggtggtcg agaccgaacc ccggcgccgc gacggccgtc cgcgccgcaa cgccgacccg
    33241 ctgagcctgg gggcgcgctg ggacctcgcg gtcgggcgca cgctggaggg cgaggcgcgc
    33301 gtgttccgcg aatccggcga catcagcgtg gagggcgagc tgcgtacccg gctcggcctg
    33361 gagaagctcg aggcggtgct gaacgcgcgc ggcgatcgca tcgacctcgc cgtcgacgtg
    33421 cagggcaagg agctcggcag cgtgcagggc gaggccgggc tgcgcgcgcg acgcggtgcc
    33481 gacggcctgt ggagcgtgcc gccggcgacc gcgatccgcg gcgcggcgcg gatcgacatg
    33541 ccctcgctcg cctggctcgg gcgcctctcg caggagagcg tcgagctcgc cgggcgcatc
    33601 cggggcgact tcaccgtggg cggcacgatc gagtcgccgc gtgcgagcgg ccgcatcgag
    33661 gggcgcgagc tcggcttcac cctcgtcgac cagggcctga tgctcgctgg tggcgagctc
    33721 gatgtcgact tcgaccagga gcgcgtgcgc ctggagcggc tcgagttcgt ctccgagaac
    33781 cgcgtgcgtc cgcgcgagaa tcgcatcccc ttcgagcgcc tcaccgccac gcccgggcgc
    33841 ttcaccgcgc gcggcgagct tgcgctcgac agcggcgcgg gcgatttccg cttcgacgcc
    33901 gaccgcctgc cgctgctgca gcgcgacgac cgctggatgc tgctgtcggg cggcggcacg
    33961 gcgcgcagca ccttcgagtc gctggcgctg gacgcggagt tccgcgccga cgccggctac
    34021 ctcgagttcg ccgaggcgct gccgcccagc ctctccgacg acgtcgtcgt gctcgggcgt
    34081 gagcccgcac cggcgcagca gggcggcggc ttcgcggtga atgcggatgt cagcgtcggc
    34141 ctcggcgacg cgctctacct ctcggcgctc ggcctcgaga cgcggctcgc cggcgggctg
    34201 cgcatccgcc tgcagccggg gcggccgctg tcggcggtgg gcacgatcga gaccgtgggc
    34261 ggcggctacc gcggctatgg gcagaacctc accctggagc gcggcctgat caacttccag
    34321 ggcccgctcg acgcgcccgg gctgaacatc gtcgcgctgc gcaagggcct ggaggtggag
    34381 gccggggtgg cgatcaccgg cagcgccaag cgcccgcaga tcaagctggt gtccgatccc
    34441 gaggtgcccg accccaccaa gctgtcgtgg atcgtgctcg ggcgcgcgcc ggacgccggc
    34501 tccggcgccg acctcggcct gctgctcccg gccgccgcgg ccctgctcgg cgggccgggc
    34561 ggcggcatga ccgaggagct ctcgcgcagc ctcggcttcg actccttctc gatcggccag
    34621 ggcgagctca acagcaccac gcgcacgcgc agcagccggg tgctcggcag cggtcagacg
    34681 gtctcgggcg ggccctcggt cgccggccag gtgctgagcc tgggcaagcg cctcgggccg
    34741 gaccttttcc tcaacttcga gcagggcctg ggcggggccg ccaccctggt caagctgacc
    34801 taccaggtct cgcgccgcct gtcggtgatc gcccgcggcg gcaccgacac cgccctcgac
    34861 ctccagtacg gcttctcctt ccgttgaggc gcgtggcggg cggggaatga ttcccctccc
    34921 gtgcggtctc tccgatgcca tgaccaaccc cgatggagcg aacagatgac agagagcaag
    34981 aacgatccgg tacgcgagac cgccatcctg ggcgggggct gtttctggtg cctggaggcg
    35041 gtgttcgacc tggtggccgg cgtggatgag gtcgtgcccg gctattgtgg cggccacgtc
    35101 gagctgccga gttaccgcga cgtgtgctcc ggcggcaccg gtcacgccga agtggtgaag
    35161 atcgtgttcg atccggcgaa ggtgagctac cgtgagctgc tcgacgtctt cttcaccatc
    35221 cacgacccca ccacggtcga ccgccagggc aacgacgtgg gcccgcagta ccgctcggtg
    35281 atcttcgcca cctccgaggc gcagctgcac accgcgctcg ccaccatcga ggcgctcggc
    35341 gagcgccgcg tgttcccgaa ggcgatcgtc acccgggtcg agcgtgcgcc gcgcttctgg
    35401 ccggccgagc cggagcacca cgactactat gtgaacaaca gcgaccagcc gtattgccag
    35461 tacgtggtgg cccccaagat cacgaaattc cgcagccact tcgacctgcg ccgctggccc
    35521 gaaggcagca ggtagaatgc gcgctttcac tcacggcagg agaacggcat gacccagacc
    35581 accaccgcca gcgggctcgt catcgatgaa ctcgagctcg gcagcggcga taccgccgag
    35641 aagggccgca tggtgtcggt gcattacacc ggctggctca ccgacgggcg caagttcgac
    35701 tcgagcaagg atcgcaacga tcccttcgtc ttcccgctcg gcgccggcca cgtgatccgc
    35761 ggctgggacg agggcgtgca gggcatgcag gtgggcggca agcgcaagct caccatcccg
    35821 cccgagctcg gctacggcgc gcgcggcgcc ggcggcgtga tcccgcccaa cgccaccctg
    35881 gtgttcgagg tcgaactgct caaggtgctg tgatccctcg cgggggcggg cgcccccgcc
    35941 gcgaggcggc gcggccgcct cgccatcgac accaggagaa cccggcgttt cgagccgggc
    36001 aaggcaggga gacatgaaac tcgacccggc gcagcgtcac atcctgaccc tgctgcaaaa
    36061 ggactccacc ctcagcacgc aggcgctcgc cgacaaggtc ggcatgtcgc cgacgccgtg
    36121 ttggcggcgt gtgcgcgaga tggaggaggc gggcgtgatc cgcggctacg tcgcgctggt
    36181 cgaccgccac aaggtgggcc tgggcacctg cgtgtgggtg cgcgtcaagc tcaagcagca
    36241 cagcgccgac gtgctcgatc gcttcgagcg cgtggtcaag gcctgcgagc aggtagtgga
    36301 gtgctacgag ctgctcggcg agaccgactg cctgctcaag ctctacctgc cgaacctcga
    36361 ggccttgtcg gtgttcatgc acgaattcct gctcaagatc cccgagatcg acgtcaccca
    36421 ttccgcggtg gccctgcgcg agatcaagaa cgagaccgcc ctgccgctgt gaggcgggca
    36481 gggcggtcgc ggtgtcggca cgatgcaggc ttcgcgccgc gcttgctgga gcgccggatc
    36541 gcgactcgcg tcgctcctac gggcgaggca ggatcgcgac tcgcgtcgct cctacgggca
    36601 gggcggaatc gcgactcgcg tcgctcctac gggcgcggct ggacgcgccg cgattttgta
    36661 ggagcgacgc aagtcgcgat cggtgcgtcg gttccnnnnn nnnnnnnnnn nnnnnnnnnn
    36721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36781 nnnnnnnnnn nnnnnggacg cgccgcgatt ttgtaggagc gacgcaagtc gcgatcggtg
    36841 cgtcggttcc tacaggttgc tccagaacac gaacagcacg cccaccggcg cgacgtagcg
    36901 cgacagcagg gtccacaagc cgaatgtggc atcgccaagg cccagttccg ccttcacgcc
    36961 ctgctggtcc agcttccacg ccgcgaacac gattgcaccc aggccggcga gcggcagcat
    37021 gaacttgctg gtcgcatagt cgagcaggtc gaagatgttc atgccgagca gcttgacgtc
    37081 gctccacacg ttgaaggaca gcagcgcggc gatgcccagc gcccagatgg tgatgccggc
    37141 gatcagcgtg gcggcgacgc ggccgagcgg ggtgcgctcc tcgatcagct ccaccaccgg
    37201 ctcgagcagc gagatcgacg aggtcagcgc ggcgaaggtc agcagcagga agaacagtgc
    37261 gccgatgacc aggccgccgc ccatgtggcc gaaggccagc ggcagggtca cgaacaccag
    37321 gccggggccg gccgcggggt ccaggccgtt ggcgaacacg atcgggaaga tcgccatgcc
    37381 ggcgcacagc gcgaacaccg tgtccatgat caccacggtg cgcgcggcgc gcagcaggtt
    37441 cacctcctgc ccgaggtagg agccgtaggc catcatgatg cccatgccca gcgacagggt
    37501 gaagaaggcg tggccgagcg cggcgagcag caccttgccg tcgagcttgc tccagtccgg
    37561 gttgaagagg taggcgaggc cctggccgaa gctgccggtg gtcatcgcgt agccgaccag
    37621 caccagcagg atcaccccga gcgccggcat catcagcttg gtgccgcgct ccaggccggc
    37681 ggacacgccg agcgccacga cgacgacggt gagcagcatg aagatcgagt gccaggtcag
    37741 cagctcggcc gggcgggcga ggaagccctc gaaggcgccg ccgatcgccg ccttgtccag
    37801 gccgatgaag gcgccggtgg cggcgtcgcg catgtaggcc agcgcccagc cgccgatcac
    37861 cgagtagaag gacaggatca ggaaggcggc gagcacgccg atcgcgccca ccacggccca
    37921 gttggtgctg tggccgttgt cgcgcgcgac ctgggccatg gtgttgatcg ggttcttctg
    37981 cccgcgccgg ccgatcatcc actccgcgac caggatgggc acgccgatga aggcgatgca
    38041 ggccaggtag accagcacga aggcggcgcc gccgctctgg ccgaccatgt agggaaactt
    38101 ccagatgttg ccgaggccga cggcggagcc ggtggcggcg agcacgaagc ccatccgcga
    38161 cgaccattgg ctgtgtgcgt gcttggccat gtcagcgttc cctgcggaga gtgggtgcga
    38221 cgggggcgtg cggccgcgcg ggggcggcgc cgcgggtggc ggcgttcggg ttcatgcagg
    38281 tctcctcgga tcgtttcacg ctcgtggcgt gtggagggaa aaggcgcgga ttctaaacgt
    38341 ccgaggcctg ccgcccaagc ggggatgcct gcgccgcgac gtgtgcggat ccgtgccgac
    38401 cccttttgtg gtcgccggac ggttgggtcc ggatggcgcg catcgtacgg cttgatgcgt
    38461 gaaataattt ttcttatatc ggcgtccgca gccccgattg cgcaaaatct tttccgccgc
    38521 tcgccctagc atgtgcatcc acgagcgccg caggccgagc cggccgcggt tgcgcaccga
    38581 cccacccgca gaggagacca cgccgacccg ccggcagccc gcgccagacg cgcaggcctg
    38641 ccgaccggcg gcggttcacg ccaccatgtc gaacatcgcc cccaccgtca gcctcgacga
    38701 taaatacacc ctcaagaccg gccgcgcctg gatgaccggc atccaggccc tggtacgcct
    38761 gccgatgatg cagaagctgc gcgacgaggc cgccggcctg aacaccgccg gcttcgtcac
    38821 cggctaccgc ggctcgccgc tcggcagcgt cgaccaggag ttctggaagg cgaagcgctt
    38881 cctcgagccg atgaacatcc gcttccagcc cgggctcaac gaggacctcg ccgcgacctc
    38941 ggtgtggggc acgcagcagc tcggcctcga ccccaacgcc aaggtcgatg gcgtgttctc
    39001 catctggtac ggcaagggac ccggcgtgga ccggacctgc gacgtcttcc gccacgccaa
    39061 cgccgccggc acctcgcagc atggcggcgt gctggtgatc gccggcgacg accacgccgc
    39121 caagtcctcg accctgccgc accagaccga gcacgtgttc aaggcgctga tgatgccggt
    39181 gctcgccccc gccggcatcc aggaatacct ggagtacggc ctgcacggtt tcgcgatgtc
    39241 gcgctactcg ggctgctggg tggcgttcaa ggcgctgacc gacacggtgg agacctcgtc
    39301 ctcggtcgac atcgatccct tccgcgtgga catccgggtc ccgagcgcgc aggagttccc
    39361 gctgccgctc gacggcctca acctgcgctg gcccgacccc ccgctggtgc aggaggagcg
    39421 cctgctccac cacaagctct acgccgcgct cgcctacggc cgcaagaacg ggctggaccg
    39481 catggtcatc gacccgccgc aggcgcgcct gggcatcctc accagcggca agagctacct
    39541 cgacgtgcgc caggcgctcg acgacctcgg catcgacgag gccaccgccg cggcgatcgg
    39601 cctgcgcctg tacaaggtcg gcatggtgtg gccgctcgac gccgagggcg tacgccgctt
    39661 cgccgacggc ctcgacgaga tcctcgtgat cgaggagaag cgccagttcc tcgagtacca
    39721 gctgaaggaa gagctctata actggaagga cgaggtgcgc ccgcgcgtga tcggcaagtt
    39781 cgacgagaag ggcgagtgga gcctgccgca cgccgactgg ctgctgcccg ccgccggcga
    39841 gctcaccccg gcgatgatcg cgcgcgcgat cgccggccgc atcgcgcgct tccacacctc
    39901 cgaccgcatc aaggcgcggc tcgccttcat cgaggccaag gaggccgcgc tcgccaggcc
    39961 gcgcctgtcc atccagcgcg tgccgcatta ctgttccggc tgcccgcaca acacctcgac
    40021 caaggtgccg cagggctcgc gcgcgattgc cggcatcggc tgccactaca tggcgacctg
    40081 gctgtcgccc gagcacacgc agaccttctg ccagatgggc ggggagggcg tgccctggat
    40141 cggccaggcg ccgttcacca acaccccgca cgtgttcgcc aacctcggcg acggcaccta
    40201 catgcactcg ggcatcctcg ccatccgcgc cgcggtggcg gcgaaggtga acatcaccta
    40261 caaggtgctc tacaacgacg cggtggcgat gaccggcggc cagccccatg acggcacgct
    40321 gtcggtgccg atcatcgccc gccagctgct cgccgagggc gtgggcacgg tggtggtggt
    40381 caacgacggc acgccgcgcg cctacggccc ggccgatctg ccgcacggcg tggcggtgcg
    40441 ccaccgcgac gagctcgacg ccgtgcagcg cgagctgcgc accgcgccgg ccgtcaccgc
    40501 gatcatctac gaccagacct gcgccgccga gaagcgccgc cgccgcaagc gcggcaagtt
    40561 ccccgacccg gccaagcgcg tggtcatcaa cgacctcgtg tgcgagggct gcggcgactg
    40621 cagcgacaag tcgaactgca tgtcggtggg cgcggtcgag accgagttcg gccgcaagcg
    40681 cttcatcgac cagtcctcgt gcaacaagga ctattcctgc gtgaagggct tctgcccgag
    40741 cttcgtcacc gtcgagggcg gcaagcttcg gcgtggcaag gcgagcggcg cgagcgccct
    40801 cggcggccgt ctcgcggacg agctgcccga gccgcagctg cccgacaccg ccgcgccctg
    40861 gggcatcctc atcaccggtg tcggcggcac cggcgtggtg accatcggcg cgctgctcgg
    40921 catggccgcg cacctggaag gcaagggcat ctcggtgctc gacatggcgg ggctggcgca
    40981 gaagggcggc gcggtgtggt cgcacgtgcg catcgccgac cgccaggagc agctccacgc
    41041 cgcgcgcgtc gccgccggcg aggccaacgc cgtgatcggc tgcgacctcg tggtggcggc
    41101 cagcgacgaa tcgctcgcca agatgcagca tgggctcacc cgcgtcctca tcaatcgcga
    41161 tcagaccaac accagcgagt tcgtgcgcgg cttcgccgcc caggcgcgca gcggcgacgt
    41221 gctcgccaac cccgacccgc agttcccggc ggcggcgatg gaggcacaga tcgtcgacgc
    41281 ggtcggtgcg gacaaggccg agtttctcga cgccaccgcg ctcgccaccg agctgctcgg
    41341 cgattcgatc gccaccaacc tgttcatgct cggcttcgcc tggcagcgcg gcctggtgcc
    41401 gctttcgcgg caggcgatcg agcgcgcgat cgagatcaac ggcaccgcga tcgaggccaa
    41461 caaggccgcc ttcctgtggg gccggcgcgc cgcggtcgac ctgcgtgcgg tgcgtgaggc
    41521 cgccaagccg cgcttcggca cgcccgcgca ccaccgcctg tcgaccagcg tcgacgagct
    41581 gatcgcgcgt cgcaagaccc atctgaccga ctaccaggat gccgcctacg ccgagcgcta
    41641 cctgcgcctg gtcgagcgcg tgcgggtcgc ggaggcgaag gtcgcgcccg gcatcaccct
    41701 gctgaccgag gcggtggcgc gcggctacca caagctgctc gcctacaagg acgagtacga
    41761 ggtcgcgcgc ctgtatacgg acagcgactt tctcgcgcgc gtcgaggagc agttcgaggg
    41821 cgactacaag ctcgtcttcc acctcgcgcc gccgatgctg gccgacaagg acccagccag
    41881 cggcgagctg aagaaaaagg cttacggtcc ctggatgctg aaggcgatgc gtgtgctggc
    41941 gaagctccgc cacctgcgcg gatcgaggct cgaccccttc gcccgcagcc acgaccgcaa
    42001 gctcgaccgc gagctgatcg ccgactacga gcgtgtggtc gacgagatcg tcgtcaacct
    42061 cgaccgcgcc aaccacgagg ccgcgatcga gctggcggcc ctccccgagc agatccgcgg
    42121 ctacggccac gtgcgcgagc gctacctcgc cggcgcacgc aggcgccagg ccgcactgct
    42181 cgaggacttc cgccaccgcc gcgcaccggg cacgccgctc gccaccaagg cgcgcaagac
    42241 catcgcgatc atcccgggct gaggcacgcc gccgta
//
LOCUS       DAOQBS010000012        40339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_487_length_40339_cov_17.094082, whole genome shotgun sequence.
ACCESSION   DAOQBS010000012 DAOQBS010000000
VERSION     DAOQBS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 40339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40339
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_487_length_40339_cov_17.094082"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKN25_02775"
     CDS             <1..214
                     /locus_tag="PKN25_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89852.1"
                     /translation="AEADAGGGVEPTFAAEDAPALSSVLRVGTGAGAESGVGWGEFVG
                     ATAAACASASRSMFSPGWLTRAGGRA"
     gene            complement(338..2623)
                     /locus_tag="PKN25_02780"
     CDS             complement(338..2623)
                     /locus_tag="PKN25_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNV89853.1"
                     /translation="MNHPAATAQRLAPADKTAPAHKSPDNPPASAATAPASRQDHAGE
                     AVTGRTPQARPGGSAAGKRPGAPGYLQARLAVSQPQDAEEREADRIAGEVSRALRTPA
                     RSASGRECRDCSGEAPHGSLEPAITPPRKPTLAPRLMRETRPGSAAGTAPASATKGAD
                     AGPHHVPVATEQRIAARRGQGAPLDPGLRAQLETRLGRDLSAVRIHTDAEADSMCASM
                     QARAFTVGNDVYFCAGSYAPASDTGLELLAHELAHVGQQADAGGPQSAAPKLQRDFWD
                     TLFGDSRAPVDPMAGCRRELDASAQWATAGPYPAAPRGIVGAGGIGGFDAQYRADPLA
                     GDGVLAIEQGVAAQFKDTLVRNAGVIAPHPDLPATPEIRTLASRMNAIPVAVVRNALL
                     ARYQWTPAEQAPWLVRLESLIETTWGGRHSFFLNRPRWNWLGAEVRVDLDIGQRAKAA
                     TDHLVVEIYKTPPGESLRSFDISHEVGSGSTTDPADQTMRLASTTAGPKEYDLLRQSV
                     EFDYDSDALTGTAIATLNGFIARFNGAVGNAAHQEVRVEIVGHTSAAGSEHYNVDLSR
                     RRAEAVRTHLERHGFANTASRVHIRARGEAEADPTQPRRASDQRADLVVDGGGRMVTA
                     AHEWGHAFGLDDEYVTGATRVGDPVDHDAMTRAMTDANGVHLPGAIKEHNGGIMSFGN
                     EVRPQHYATFHHALASVTAQSPWALGLHKPKWQVAMECGAPSPPGDWPVPAPARPVPG
                     AGPANPVPGTTPGNDDGSAVA"
     gene            complement(2620..4656)
                     /locus_tag="PKN25_02785"
     CDS             complement(2620..4656)
                     /locus_tag="PKN25_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV89854.1"
                     /translation="MNAPASLRPESAPASEGARLSRAFVHAGIAEFARLSVGAPSAVF
                     EALAASQGGEDLATLLGTPPQDWRAACIALAQAAPELHGPLGRLLADLKLQLHEWFAM
                     ALCGENESSYLLNLALAELQSPGALRPGLHLLSAASAALFGAPLAPLALPNHRLVRAG
                     VLEIVGEGPMPTRSLAVAPELWALLCGDPTAWRDTAPLADGDTMLPRGFREQIPTLAT
                     MLRSGATRHVMFRGARAAGLAAAARLAAAAGRPPLQMEAATWTRQPALAAACRYAGWL
                     PVLTLELGPGERHVLPEHPALAVPMLLVGGRDGAIEASALIEIDLPPLSAQERREAWR
                     EALASEDGDGSPCLDEDRLCSFAEHALLDGPSVRTLAGRMRLDARVRGEAPGLGHLRR
                     ARAGFGSERLRQLAQPVAREVPHDALVLPDDLLERFAALVARCRQRETLSDGLGSSLA
                     EPARGVRALFCGESGAGKTLAASRLATILGAPLFRVDLSAVMNKYIGESEKNLGRLLD
                     EAAALDVILLIDEADALFGRRSEGKETGERYANMLTNFLLTRIEQHPGIVVLTSNNRG
                     RIDSAFTRRFDVIIDFPPPGANERLRLWESHLGGRAPDAALCRQLASYCTLPGGYIRN
                     AVLQAAALDPVGDAGAGAPARPIAAERLVAALLEEYRKIGRTPPPQLLNARSAP"
     gene            complement(4653..6341)
                     /locus_tag="PKN25_02790"
     CDS             complement(4653..6341)
                     /locus_tag="PKN25_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89855.1"
                     /translation="MIALADLHIGRLTLRGSATAIAHARATLPPALARARWPDEEAIL
                     VVRRIAVGGTGTELPARAAALAGQLAGRAVDPWTPGADGAEVVRFRDARDYRACLVRD
                     LLAGTAARRWLWRHRAALLAHPTATALAELLGEDALALPALLEHPPLRACLPTLWCRL
                     DAAAARSLLRAIGAATGWHGAISDALASRAAEAAGSGADLMTEAQAHHERISGDEIRP
                     GPEPGPSPPPTGPAATRAGSPRRPAPPGAAAHGTLPASDPLVVLEALLTLWRCTPAAL
                     AGPQGGEVLRGAAAALAELGGGQDAESATARASGPGRARTAGGDLPDARRRRDAGAGS
                     IPAGSPASRGFAPPASIRVGASPRAIAAVNARPAAPPRAGAPSEVTDPFAVTVTATQA
                     EVDFHTGRGGLFFLLNVLNHPGLRAWRGTLHEPHAGWRELVRLALRLELAPDAPLTGF
                     LAEACALEGEQDPVAKLAGLAKLPARADPVAVDRAVLRHVGDAALRALRAERPARVRA
                     SPSHVDVHLRLAEVDLELRRGGLDLDPGWLPWLGRVVRFHYDRSDAADRGGSSP"
     gene            complement(6347..8239)
                     /locus_tag="PKN25_02795"
     CDS             complement(6347..8239)
                     /locus_tag="PKN25_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89856.1"
                     /translation="MSDILKRFTTDASAFTELKFVDAKGTAAAVDLADATRGEATISA
                     GMLAALADLQPKAAPDEPLTEPVFSKATSTGRGMLAHVERVHDSFLNVRRDLLELRAR
                     ANARQNQAKSLIDRVCDFLDDYLKPEELRRLGVIDDEAQARPFRLLVAPDLHNAEGVL
                     LRPRLDLALPRPGEDDIEGVKHNDESALFHAGKVVADDIRQVEALRGALTTLRRRHQE
                     ALEDLRTRLVALEAELPGELRRLDVLERERTETLDDYAVAQRLLAEHWREVEAAHAER
                     RRIIEAHQGLFYVKVRETPLGRSLPDPLELRPSSPDELVPGCAGRDTALPAALAPFIE
                     AVYDIPAADWAHLRPLGHLLPGRTILAGLVEMRRQKLALRLNRPPDAGLALLSGLVQQ
                     NKALVRDIAARPFSAGALGELQRQAGAILALDDLLAIPSPQLRDPARALHQRLDTAAG
                     CLLERLRTISPSIRLNWASAAEADRLAVEAPERWPGLAEAEERDFNGVRTLVELVAWW
                     FRQLDADASAAAHGAMRNLVRACLLLAASDDPQQLVQGRLQSIPGRFRLGEALRLKLD
                     REAAPGTLLQLFDDDQRVIATLRVDDHDDQGTVASVAAILDPELERNPGAVLATGLHI
                     SGVDRG"
     gene            complement(8236..9975)
                     /locus_tag="PKN25_02800"
     CDS             complement(8236..9975)
                     /locus_tag="PKN25_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89857.1"
                     /translation="MATLSFIPPGLLAALASRPAAALLPRTPPSIRLHEPQRRPDDPR
                     RINLFPGRALGEREFERLQAYVDDRIAPLLASLAPGIVEGLACALFGTGTDTRVRVGP
                     GLAVGAAGQLVRLFYPLDQAWSELVAQAERDQDAPLRDGLFLLTIRSAVEEIDDATAA
                     EPCTRSEPDPLRERRIETVSLLGLQRIAGSPRMMAMPQARAANRIGVRLLRESPFRAD
                     SGGVPLGLAKIVGKLPVWFDACAGRLLAEPDAAAHSLLAHAVATLETWQRAHPAPLPA
                     VATQTLDELLGLDFLPAAGPLPAALLRDPAARSPSLAFLPGDLRVELAPVPAGTVEEV
                     VVGELGRGPVDLIHGLGEHLRLLLAIPDLDYRRDLFDLPQRDTRLEDELFRRGERAAH
                     AYQAWKARWYALFHGLSGAQLEALRAPVLLTKAPPEPGQYRTKLVTDRAKTLRPAPQG
                     TTSHPLADLATEGTAALPEPYASHLADPHPAPDDYQPVPDIQPAGNGLLHRREALRAD
                     IRHLEAALDKSFRLLEEVGDFLGLQRQQLDALTVSFSTLAGGVPGDGLGSQLVRWTAK
                     ANFVPKPPTENPS"
     gene            complement(10032..12221)
                     /locus_tag="PKN25_02805"
     CDS             complement(10032..12221)
                     /locus_tag="PKN25_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HNV89858.1"
                     /translation="MANIHFTPLRIGTSNAAGERISSVDPLLSRTNYFDGQLLKAADL
                     ARDQIYLDERLLELGQVFGAGIVRGLEPSLHSGHLLEVSPGIAIAPSGRVLQLSNTAL
                     RIDLLDSGLIATLNDGRFRGPARGLHALALTHTQVVDGVAEAFPKDLSTARVPHVAAW
                     AEGVELRLMPLPLPLPRQDEIAVRASLARELLSGGERLALPSDDAVALGLIAVERGRI
                     LWLDRGLLRRPQRPPNTPDAIQQDLATHYQELMQAVLAARRSAGLRGAFAAGQYFRVL
                     PPWGPLPQEAIDPVGGRQQFFPQDYEVAIAPVRRSELAAVLADSARLAPIDLERDADC
                     DVMVLVPMSDSAFALRARQLEAPAEFRPRALGRLANLDRLALRILPRPPIHRVDTDAD
                     VWRAIWAEADPAELMYVRRPPRTAETGVSALVLALGASLPPPGSALPPDAAALEERLD
                     AALEAVDAAAQARTTLENEAARLRLRIAKLEKALAATGTGVPDDLLAEVERLGLALET
                     ARKEIAALEAEDRNAAILANALEAASDRIDALKLALDAANAEIERLKTASAPVDETLE
                     ARLEELVAALGSERTRAAALEQERTALSTRLAVAEATIGRLEREAAQAGDLATQLPLA
                     LLAQARGADDAGIKAATGADKLIGADARARIAAVQIVGMADRRLDPALWPTLPALARL
                     DPAVFVKLRDHFLAAAGQLDLAKFFLEEGPGLGLSGALLELWKRVLG"
     gene            complement(12231..13169)
                     /locus_tag="PKN25_02810"
     CDS             complement(12231..13169)
                     /locus_tag="PKN25_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89859.1"
                     /translation="MSEPDDKRDTSLASDGRFEEMLKRVNYAPGMLLGIEATLAEQDY
                     HRRRATRHGYWLHGAGTVAGLRVSLQSKDPGNDTENVRVRLVVSPGIGVDGLGRELSV
                     AEPYCVDLGAWLTTQHEEPERWNALIRDGYAADDNLLWLKVTMRYQDCASGLQPVLAT
                     ELNAGTDPVQPSRVADCVLFELVAERPDDAPAEEHLFAAHARIRPYDEIEDKLGERER
                     AQVEAATGGARAQLELGARLLHSLGDDNRALVARQAFLTDPAALARTLLARIAIRLAP
                     PAPDPDLIVNPRRIEIDNLVRPFLFNAVALARLIRA"
     gene            complement(13192..15621)
                     /locus_tag="PKN25_02815"
     CDS             complement(13192..15621)
                     /locus_tag="PKN25_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89860.1"
                     /translation="MNSNGTRFLLLDGAADFHNTSRQCSWDHEQRAFTLTRQDAPRLP
                     RLAAARARERLLAATPWMLDDHGQLGRLSDDGLRLECAPSWPPRTWQPVRATLDETHA
                     DASALEVLVLDPVDAPAGRFTDLAFGGSGLVVLPWSDGGVQHGLTAVHLRRRWQARCA
                     LPFAPRRAWVEAASGTDRVWLLGETQLGLAVGAPLPQPYRGRPERFEPLQTNPDPLRL
                     AWTLALPPHGGLLGLCTDEDHLFVLGETPDSTADAPRMQIFMRALGAAPGEGFDIRRL
                     PDGLPLATDLAAAGEGRLLLLPPIDEGAEPGTRRDCPLISMREDAPAAELVPERWPRR
                     PAAALPAADRFVRHRDGRPRALSADGPVRLYRLAQARFAPNGTVTLGTPLDSAMPDTL
                     WDRIFIDACIPPGCRIDFAVQAGDDRENLPAEWIAQPQPVLTAVSSELPFASGRAPGS
                     GDHADRSGLFELLIQRASGAVREVRGRYLRLRITMHGDGRHSPAIFALRVQYPRFSWQ
                     THYLPEHFQQQERPLASAEANQAEANGADFRERLLASFEGLLTPIEDRIAAAEILLDP
                     AVAPVAHLPGLAAMLGTTLPPHWPEARRRRWLGAQGMLQQSHGSYRGLLLALDILTDG
                     AVARGAVIPVEHFRLRRTMATILGVDMDDRDHPLTLGTGLSGNSLVGDSLILSDDLAR
                     EFLALFAPEVAEAKGEAAVVERFFEEAARRMTVILHGPARRLAAVVRDALPALVPATV
                     QWAIRSSEHPFVPGLSPLLGIDTWLEASPPARPVVLDRTRLGRGDLLHNPVALDPEHA
                     VPIDATVLDAP"
     gene            complement(15636..17828)
                     /locus_tag="PKN25_02820"
     CDS             complement(15636..17828)
                     /locus_tag="PKN25_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HNV89861.1"
                     /translation="MSPPKPFPVPDLDDRRFDELVAELQARLARHVPELAPLAPGDPV
                     FALVDLFAWLTETILYRANRIPDRQRLAFLNLLQIPLRPARPASGIVSIDSTAHALPL
                     PIPPEALLKAGTTSFSTIGEVQPTPLTLRIVVKRRLDEAALAAEGISLDQLRAQYGVE
                     PAAFRPVTLHPGRDRPSAAGTCDGALHLGICLARPAPATQAERVRRALGGVLLNVGLA
                     PDTELEGGLATGLRARRLTWELAWWPDPEDAPQRVQYLPLEVVADSSAGGRRTGVARL
                     RLPRDAEMLRATSALDPQFAGLGDTPPEAPADLRPDQLLFWLRLSCAEEPDLTLGYLG
                     LNAVEVIGQGIARDVLLGAGTGRPDQRLELPDPDVDERSVEIEVEARRAFRAWTRVAH
                     FAGSGPDDTVFVVDSADRSVHFGDGVRGMRPPAGARIRAAFYRHGGGSGGNLPPGSIR
                     ELHGASEKLAVRHEWPTRGGVDAESLDEAERRIPAFLSHRDRAVTAEDFAVLARDNPI
                     NPVARAEAVAGFFPGASLAAVRRNVPGVVSVFVMPPAPQAMAAAPRPNAGLLADVHAY
                     LSARCMLGTELYVLSPRFVPIALALSLEVVDPTSEQQVHAEVERALLRYLWALPPGGP
                     RGAGWPRGKAVEINELRTQAGRVEGVEAVNALRLFYRTTEQDPPSWRELTGVQALTLL
                     DFELPELMAVSIRAGEDRPAPPRGFGPEAGAAADGPRPVPVPVIPDLC"
     gene            complement(17946..18335)
                     /locus_tag="PKN25_02825"
     CDS             complement(17946..18335)
                     /locus_tag="PKN25_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HNV89862.1"
                     /translation="MPTPESLLTSFPLLASPVAGGRQHWQTGNASVREVMLNILLTRP
                     GERLMRPEFGAGIRSFIHHPNNETTRALIADAALRALRRWEPRVSIEAVRAVADPERL
                     SQVNLEVRYRLLADERRETLQLALDLG"
     gene            complement(18355..20007)
                     /locus_tag="PKN25_02830"
     CDS             complement(18355..20007)
                     /locus_tag="PKN25_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89863.1"
                     /translation="MVSIDSALRAERPMRPARGAPSAAPHQPQVIAPFFAGEGEGLTL
                     HRYFDEDFVARFLNDAQAGRLTGTAAQPWFRADRFGRFGAEPTLRLPLHRCFYLVCAE
                     VRCMAAGGPAFDPRKILGAGLVVRRVAADGSAQRWMVADGQPLGWRDGRIPDHDPDDV
                     HRLRARGLLPVRFPEPPYSGEEVAPLHTLLATRRDARGVERKHTLLWGYLPLSGSARV
                     QAEAPLPQTDGGDAPDFGLEHAWPLGTRDARPWVDGDGLVVTEGVASVGFVELLQTLV
                     AQFRIDDATDPDNAGLRTLLGQMRFHHFEVRLVEGEGFPQRVPVFGDTALEWLDRSAA
                     ALVEFFARIDRGEFVAGRTALPDGTGGSRKDRLLLSEQQAENLRSLVGLRLARAQVRF
                     DDGLPMPRYGQGDDDRFTALPFIRWHDECGCERVAWGPASHVFRVASPFDPEAQRPTT
                     VVLPALDDLKRGLPRGVAMLAPKSLADVLRKISPDADMKGDGPGNRSGACWSFSFSLP
                     AITLCATMLLMVVINLLNLFLGWLPRVFLALPRLCLKALRGK"
     gene            complement(20001..20639)
                     /locus_tag="PKN25_02835"
     CDS             complement(20001..20639)
                     /locus_tag="PKN25_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HNV89864.1"
                     /translation="MSRHSPGPLQELHLGVVLDNDDPEDRGRVKLHLAATGLETWAAV
                     MVPSAGGGYGVSLLPRVGEQVVVAFVAPDLPIVLGAVWSGGASQPEDARPVDERYLVQ
                     SPAGLKVLLDDRQPSVTIETPAGNRLVITDQGGGKVTIEQGGERVDIAPGTVKLTGSG
                     IVSIEAAQVTISAGLVKVDAGLSKFSGVVQCDTLISNAVVSTSYSPGAGNLW"
     gene            complement(20653..21702)
                     /locus_tag="PKN25_02840"
     CDS             complement(20653..21702)
                     /locus_tag="PKN25_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HNV89865.1"
                     /translation="MPSTSAALLSVRPRMRVDGDDRPALGTAVQALSVHLPASGMASA
                     EARVVNWAAGEGAAPDFAFQDLRLGSSIEILLGEHASGAVFVGEITALEERYGDGAPQ
                     LVILAEDALHRLARRRASRVFENRGLAAVVRAIADDAGLQADVGIEVGSADWLQHNES
                     DLAFLLRVLGPHDIPLRIQDGRLHARAEEADPDPVRIDAGANARRIRIIADLNQQPRE
                     VLAHGYNLATDSSTQGSSAALAPAPPGRSAATTLAELGWEGRSPRPHPFPRTQAEAES
                     LARGAFRRDARRFLHGEIVCTGTPELGVGRELELTGVSPRLAGRYRIAECWHHFDNTQ
                     GLSTRLRVERPDWTP"
     gene            complement(21689..21853)
                     /locus_tag="PKN25_02845"
     CDS             complement(21689..21853)
                     /locus_tag="PKN25_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89866.1"
                     /translation="MPLIIDELVAEVQAEPNPAQASATEARPPAGPPGDTLLDLLALA
                     GEREARLAID"
     gene            complement(21853..22572)
                     /locus_tag="PKN25_02850"
     CDS             complement(21853..22572)
                     /locus_tag="PKN25_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89867.1"
                     /translation="MPAPQIARAKLIPMNGDQVETDESKHIDVQFNPATLRVTLSNTL
                     KADNRAGSGGSGAAAQYIDKSESTLAVELVFDTSVAAKLGSDARANRETVAADSDVRT
                     LTKRIAETFMKPQDAGSNRPRAPGRCRFQWGSFQFTGMLSSYSETLDFFAPEGIPLRA
                     TLALTFKEDRYQFALDPTVRADARPMPGFAAGGEGRDAASAAQSAGRDPRDWRVIAEL
                     NRLDNPRFTVPGALLIPTGGT"
     gene            complement(22587..23051)
                     /locus_tag="PKN25_02855"
     CDS             complement(22587..23051)
                     /locus_tag="PKN25_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNV89868.1"
                     /translation="MADSAGEFIPFRFRVALYDGEGGRLLCRGAFSEVSGLEATMAPK
                     TLREGGRNWGEVQLAGPTSFAPVILKRGITEVADLWNWFDVSTRQSNHALRLSGTIDV
                     YHPQDPDKPLLRWRITNALPTRFKGPDLSSTASQVAIEELQLVHEGLELERG"
     gene            complement(23044..24651)
                     /locus_tag="PKN25_02860"
     CDS             complement(23044..24651)
                     /locus_tag="PKN25_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89869.1"
                     /translation="MLLADKRIELDARPTARRLAPVEVVLIGHCPSLDGRARWDRLAR
                     DPAVLGEIAALGFGAEVERLRALAAGATAGEAGDEALLALYRLPIPIRSLGHFQVLFP
                     EAFAVACGYRSTLAGDAAWLPMAVQDFFATDERGFEDTRALWVIRVPEAEGFAAFLPR
                     PDASLAEPAGLGAFERALLVERAGILALPDLERLLIPHALPDLPRVRLANPEPAFLPC
                     GTEVDDTHRERRRAGERPPAETPRVPADIVPPILRTLARLRPDVQCLLTLPLDHQPQD
                     ELPAPARGFLAHVARIVGDAGAEPAGEADDAPHAELAGKLRHLQLLWPYLRSADRPLA
                     PACGLIAGMQAAVSLRYGAWRSIAGRPLPGKALPWPPPTQQAATALRATPGATVVLTR
                     AGRLQVDDEALCVPCLPGADLQRMTKAQRGLEHWRSAEVMRFMGWLRRELQALGERLL
                     FDTDPGDPRPELALRGFFGRLHEAGALRGAQPEHAYRIRELPAVDSAIAFEIEIAPAY
                     PIDHLRLTFLHDRHAAVTETRIEAIDG"
     gene            complement(24656..25114)
                     /locus_tag="PKN25_02865"
     CDS             complement(24656..25114)
                     /locus_tag="PKN25_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNV89870.1"
                     /translation="MAVFRETPYSAFNFLVDLEPGQGEEVQAGFSEVSGLNAEITVAE
                     YRAGNDRVNYVRKVPGIHKAGDVTLKRGVIGAQNLYEWLELGRTGKLAQVKRDIVVKL
                     QSEDRAGTVVSWKLRGAMPIKWTGPTLTAKGGGDIAIEELVLSVETIEQE"
     gene            complement(25132..27306)
                     /locus_tag="PKN25_02870"
     CDS             complement(25132..27306)
                     /locus_tag="PKN25_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HNV89871.1"
                     /translation="MPEYLAPGVFVEETSFRNKSIEGVGTSVAALVGPTRSGPLRGLP
                     EVLTSYADFERIYGDAHDLAFGADGKAVLNHTAHAARAFFDNGGKQLFVARVMQGVNG
                     LAADAAGVWPGAAMKADAGNTLRFFSRFPGRMGRYALELRWRDSENLLRSETAASARA
                     GALYFLEARGVTLAAKAAGPIDDAAFPVDLRAIVRLDGANLAIQDGRATIVSRADEDA
                     PAALAANQITSLIPAQLPAGARLTRVFARPPASGALAAGTPAELRLAEVVDLAAFTGG
                     AGWGSRKAVRGTLDAAGEVFTATPALNPGLAEAVTLPLAALAAAPGAARALFVLRSFD
                     VDVRNGGKDGEVIRTYPGLTTAATGPTSLAAGLAVTPDRRAEALSSPVACHLAANATD
                     GAILAALLSICAPAARDPGEASLDEPRYLIELEGGSDGDEPGAIDYAGEADETKGSTG
                     LKALEDVEDVAIVMTPAAAGSAEDVHKAVVMEVQKHCRRMRYRIGIVDARAGQSLGEL
                     RAFAGNFDDSRLALYHPWVVIPDPTRTRRDITVPPAGFIAGVYARTDVDRGVHKAPAN
                     EIVMGALRFDQQINAFQQELLNPNGINCLRSFAGRGHRVWGGRTLSSDPEWKYVNVRR
                     YFLYLERSIEKSTQWAVFEPNGEALWANIRSSVEDFLFAEWRNGRLLGATPKEAFFVR
                     CDRSTMTQNDIDNGRMVCLVGVAALKPAEFVIFRIGQKTADA"
     gene            complement(27351..28118)
                     /locus_tag="PKN25_02875"
     CDS             complement(27351..28118)
                     /locus_tag="PKN25_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89872.1"
                     /translation="MIVETLVLHASDFIEWEDASGAQGGARRPVCVRAFRIQFDDAPA
                     DLELVHKNTGTALWRRVPAGLRQVIDGEADAGALQPPDEPLYALSGALLDPAGHYLPR
                     RFALDAGRRTAHKLRLYRSPLGTRFGRAGGLVGRTALEDGSAVPWALLALRVTPPLAP
                     PFDFVAQSDARGEFRLALERLPALTKDAPAASYPGLLEVRAAAAGTNPDTLPVARIRG
                     NGSGDGAFKTQLVVDIAPGRIATLASPGREALVLRFP"
     gene            complement(28115..28711)
                     /locus_tag="PKN25_02880"
     CDS             complement(28115..28711)
                     /locus_tag="PKN25_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HNV89873.1"
                     /translation="MASHLGILSALRALRERLAIRMAVRLGGNPQVEILGSQTLAGKP
                     QANTDTLGLYLHRISVDPFSRNRHLPPQPGRHLPRPELPVNLHVLLVAWCTHTEQEIE
                     YLAAAIQIIGAGLVLESADMSLADPDWGPEDVVQVLPEEMSTEDLMRLWDSLPDDYRL
                     SAPYLVKTVRLAPDLEREEGPPVKTLVFPMHVPDGSAR"
     gene            complement(28953..29141)
                     /locus_tag="PKN25_02885"
     CDS             complement(28953..29141)
                     /locus_tag="PKN25_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89874.1"
                     /translation="MNFPEHGAMTPCLLVRAAAGSGKRLEQDRQAAQGETDVTVRLQP
                     RLGARLAQVAWRSTGGAG"
     gene            complement(29155..29490)
                     /locus_tag="PKN25_02890"
     CDS             complement(29155..29490)
                     /locus_tag="PKN25_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89875.1"
                     /translation="MHELYNRADPAYTGRATVPVLRDRSRHTIVKNESADILQILNSD
                     FGALAHNDIDLRPLSADPNMMAYLRRLLALPAFAGSTRADHIKAGYYSIRALNPTGIV
                     PAGPLLHDL"
     gene            complement(29608..30507)
                     /locus_tag="PKN25_02895"
     CDS             complement(29608..30507)
                     /locus_tag="PKN25_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV89876.1"
                     /translation="MDRFVEMQTFCAVVDAGSFVGAAEALGLSKATASRYVGELETRL
                     GVRLLHRTTRRLSLTEDGERFYARSREVLAGLEEAEAELHSRSGAARGLLRINAPVTF
                     GIRHLAPLWGAFRDLHPQVRLEVTLADRTVDLVEEGFDIAIRIAALPSSTLVCRQLAR
                     TRIVLCAAPAYLAAHGAPRLPADLASHTVIAYSYWSGGDEWQFDGPDGRVSVRTRPAV
                     HTNSGDTCRAMALAGQGVILQPGFLVAEDLAAGALVELMPAYRSVTLGIYALYPTRKF
                     VPPKVRALVDFLAERFAAPPPGW"
     gene            30787..31614
                     /locus_tag="PKN25_02900"
     CDS             30787..31614
                     /locus_tag="PKN25_02900"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNV89877.1"
                     /translation="MQMLPTLFVSHGAPTFALDPGLAGANLATLGRRLPRPHAVLVVS
                     PHWMTRQPQVTLSLRPATIHDFGGFDATLYTLQYPAEGAHLAARRALELLQAAGLPAT
                     PNADRGLDHGAWVPLSHLYPKADIPVFQVSLPEWLDADGAYDYGRVLAPLAGEGVLIV
                     GSGSLTHNLYEFRLGDPRAQAYAAEFAHWVRDAVLAGDHPRLRQALEIAPHARRAHPT
                     AEHYLPLLVAAGAAAQAVPASVIEGGIVHGVLSMDSFLFGTDLAAMKGADANQALPD"
     gene            31624..32244
                     /gene="ypfH"
                     /locus_tag="PKN25_02905"
     CDS             31624..32244
                     /gene="ypfH"
                     /locus_tag="PKN25_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HNV89878.1"
                     /translation="MSDQDLIVQQASAPAARLILLFHGVGSRPEDLVPLGRLLAEADP
                     GAWVVSVRSPDPGDLGGGWQWFSVRGVTEDSRPARIAEAMPSFVRQVRDWQARVAVDA
                     AATTLVGFSQGAIMALESTQLPETVAARVISIAGRFALPPRVAPANTQVHLLHGEQDP
                     VIDARYSLMAAETMQALGAAVSVEFFPDLGHGVDARVAGRVKALAA"
     gene            complement(32399..32474)
                     /locus_tag="PKN25_02910"
     tRNA            complement(32399..32474)
                     /locus_tag="PKN25_02910"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32439..32441),aa:His,seq:gtg)
     gene            complement(32518..32594)
                     /locus_tag="PKN25_02915"
     tRNA            complement(32518..32594)
                     /locus_tag="PKN25_02915"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32558..32560),aa:Arg,seq:tct)
     gene            complement(32622..32698)
                     /locus_tag="PKN25_02920"
     tRNA            complement(32622..32698)
                     /locus_tag="PKN25_02920"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32662..32664),aa:Pro,seq:tgg)
     gene            complement(32770..34068)
                     /locus_tag="PKN25_02925"
     CDS             complement(32770..34068)
                     /locus_tag="PKN25_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89879.1"
                     /translation="MMGPGLSYDDTPPFSAPIRFFLTAPLFGVAAGLTLIFGGEILVS
                     RWTPGALAITHLFAAGFMLQVMLGALLQVMPVVAGASMPAPLRIAGITHLAMALGAAG
                     LAFGLGAGAPGVLLGGAVLLVGGLFVFLSAAALGLSRGARTGNNTRTPRDLRMAFAGL
                     VVTAVLGFSLVLVLTRGVALPVPLPTVVNLHAGWGWMGWAAVLLAAASWVVVPMFQIT
                     AAYPQRFTTLWAPAVTATLVLWTLAEYLAIDAARFIAILALGLLGAGYAGTTLYLQAH
                     SRRSKPDTPFLAFREAMYSALAGVLVLAISLWSDADCWPILAGVLILHGGFGGTITAM
                     LYKIVPFLAWLHLTQAGLKAPNMKKLLPDTPIRRQLRVRTTSLATLCVAVFVPVLAPL
                     AGVVLAVEFGWLFANLLRVVRARRDAAKNAGPRPGSHASA"
     gene            complement(34065..34283)
                     /locus_tag="PKN25_02930"
     CDS             complement(34065..34283)
                     /locus_tag="PKN25_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNV89880.1"
                     /translation="MSGRVVDARGLEPPEPFERAMEGLMALGKDEEMLLILDRMPHPL
                     LRILDRDGYRHAEAFRDDGAVEIRIRQR"
     gene            complement(34280..34738)
                     /locus_tag="PKN25_02935"
     CDS             complement(34280..34738)
                     /locus_tag="PKN25_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV89881.1"
                     /translation="MNTISELMTHDHRDCDGIFARAEELASDGDWADAAEALKKFADA
                     LNAHFDAEESTLFPAFEQATGMTQGPTMVMRHEHQNMRDTLAALQYALDKQDADDFAG
                     EAETLLIMMQQHNMKEESVIYPMLDSRLPGDEQERLSASVGKHLGEARAA"
     gene            34921..36189
                     /locus_tag="PKN25_02940"
     CDS             34921..36189
                     /locus_tag="PKN25_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV89882.1"
                     /translation="MVRTRRSGGAENGCKGGSTSGWVRVFVPFALGYYLSYLLRTVNA
                     VISPRLTDELGLSAASLGLLTSAYFLAFGAAQIPVGIALDRFGPRRVEAALLLLAALG
                     SALFAMADSLGALAGARAMVGLGVSACLMAALKGFTLWYPPERQSSMIGFIMASGSLG
                     ALTASVPLAAVLPVLGWRGAFWIIAAVSLFAAVLALRALPDSPAARSEASLAAALRVV
                     AGIFAAPAFLRHAAASVFFVGGFMALQSLWVVPWVMNVNGFDIAGAATVLVALNLGNL
                     AGQLAVGVFGVRLARAGVQPLGLLRAGYGMMLVVQALILWSDLPVLPLWTAFGLLTAA
                     NSQIYLSAARAFPPALFGRVSTALNLMAFAGAFCVQWGFGIGLDLMRGMGVELARALA
                     WGFGALLALQALAYLPLLGRPAARPSKGEA"
     gene            36359..40159
                     /locus_tag="PKN25_02945"
     CDS             36359..40159
                     /locus_tag="PKN25_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNV89883.1"
                     /translation="MSEPTTDSTVQPPRRKGLRRTLWTVAILLALSAAAYLAVPPVAR
                     HYAQKLLGETLGREVVVERVLINPFRLTAEVGGLRIMEADGSAEALGFESLRANLELE
                     SLLRKGIVLHELALDKPRVHVALEAEGRHNWTDVLERIAALGGEEPAEDTPPTLFSIG
                     NIRISDGLVRVDDRVRGITHELSELGVGVPFVSNLPVRVDVFVQPSLSAKLNGDALLL
                     NARTKPFAESQETILDVSLKEFDLTPWIAYLPIEPRFRLPSALLTTNLELSFSQTVEG
                     APVLSLRGPLQVHKLVLQDREGAPVATADEVELEFADVQPMIGRWHFTRLRLARPELD
                     LVRLKDGGLNVLGLISPPAAEGARKAKGKAAKGGKAAAQVAQKAASGPAAGAAQADAG
                     VTTPAAPADGKTVADARDATPAGADFLLALTRIRDGVVRFEDRGLAEPFRARIEAINL
                     DMRDLATEGELPAEIRLDYVTDAGEKFTHEDVLRLAPFEYSGSLQFESIRLPRYAPYL
                     AAAMPGASIGEGSLSGGAQYRMTLGKDGAPAVQVSAEALALRDFALLLKDAKTPALSL
                     PALDVRSATVDLAGRSVRIGGVALQGAAVSAVRTKDGEFDLVRALVPPPAKGAASAPA
                     AKDKAEPDWVIAVDKFALRGASVRLDDRSVARPVLTTLEGIDFELDGFSTAKGDAIQF
                     KLDSMLNKRGKVAASGPLTIAPLKADLALDLRNVDLLPLQPYVLEQTKIAISRGNLTT
                     KGRLKLDTDRRGKLLASFSGDVGVANFASVDRLNATDFLRWRTLAIGGIDARLEPLSL
                     GVKRIALDDFYTRLILDSQGRLNLREIGGVKGESEAAAKPAAAVAHTKEVEQGEMQGG
                     AAADGQRTAPIAPPGPPPPPVRIDRIELKRGNVAFSDRFVRPNYDANLTGLAGTITGF
                     STSSDKLARVEIVGKVDKSAPLSITGELNPFRQDAHLDILASVKDFELTGLSSYSGKY
                     VGYGIAKGKLSAELNYKIIDRQLTATNRIFLDQLTFGDAVDSPDAVNLPVQLAVSLLK
                     NSRGEIDLNLPVSGTLDDPQFSVFGLVMKMLFNLIGKAITSPFALLGSALGGGEELSQ
                     LDLAAGTATLGEAEQGRLKTLAQALVDRPTLRLDIVGRADPASDLDGLRQAALTNAVR
                     AQKLKAMIAKGQAAPSIEEVEVGEAEYAELLKKAYRAADFKKERNLVGMVKDIPVPEM
                     EALMRANAKVGDDDLLALARARAQVVRDWLAGEGGVPGERVFVLEPKVEALGEGGEVQ
                     FSLR"
BASE COUNT         5449 a        13568 c        15436 g         5886 t
ORIGIN      
        1 ggcggaggcg gatgcgggtg ggggggtgga gccgacgttc gcagccgagg acgcgcccgc
       61 gctgtcttcg gtgctgcggg tcggtactgg cgcgggggcc gagtccggcg tcggatgggg
      121 cgagttcgtc ggggcgacgg cggcggcgtg cgcctcggcc tcgcgctcga tgttctcgcc
      181 gggctggctc acgcgtgcgg ggggacgtgc gtaggccggc atggacggtg gaggcaggcg
      241 gctcgcggcc gtcgcgggtg ccgccgggcc ggcacggccg gtcgtggctg caggtcgggt
      301 ggccggcgcg ggccgttcgg caggccggtg cgcgagctta ggcgacggcg cttccatcgt
      361 cgttgcccgg ggtggtaccc ggcaccgggt tggcggggcc ggcgccggga accggccggg
      421 ccggggccgg caccggccag tcgcccggtg gcgagggggc gccgcattcc attgcaactt
      481 gccatttcgg cttgtgcaat cccagcgccc agggcgactg cgcggtgacg ctggcgagtg
      541 cgtgatggaa ggtcgcgtag tgttgcggac gcacctcgtt gccgaaggac atgatgccgc
      601 cgttgtgttc cttgatcgca ccgggcagat ggacgccgtt ggcgtcggtc atcgcccggg
      661 tcatcgcgtc gtggtccacc gggtcgccga cgcgggtggc gccggtcacg tactcgtcgt
      721 ccaggccgaa ggcgtgtccc cactcgtggg ccgccgtcac catccgcccg ccgccatcga
      781 cgacgaggtc ggcgcgctgg tcgctggcgc gtcggggctg cgtcggatcg gcctcggcct
      841 cgccgcgcgc gcggatgtgc acacggctgg cggtgttcgc gaagccgtgg cgctcgagat
      901 gcgtgcgcac ggcctcggcg cgccgtcgcg agaggtcgac gttgtagtgc tcgctgccgg
      961 cggcgctggt gtggccgacg atctcgacgc gcacctcctg gtgggcagcg ttgccgactg
     1021 caccgttgaa gcgtgcgatg aagccgttga gcgttgcgat cgcggtgccc gtgagcgcgt
     1081 ccgagtcgta gtcgaactcg acggactggc gcagcaggtc gtactccttg gggccggccg
     1141 tggtgctggc gagccgcatc gtctggtcgg cagggtcggt cgtgctgccc gaaccgacct
     1201 cgtgcgagat gtcgaagctg cgcaggctct cgcctggcgg ggtcttgtag atctccacga
     1261 cgaggtggtc ggtggccgcc ttcgcgcgct ggccgatgtc gaggtccacg cgcacttcgg
     1321 caccgagcca gttccatcgt ggcctgttga ggaagaagct gtgccggccg ccccaggtcg
     1381 tctcgatcag cgattccagg cggacgagcc agggcgcctg ctccgcaggc gtccactggt
     1441 agcgtgcgag cagcgcattg cgcaccacgg cgaccgggat cgcgttcatc cggctggcga
     1501 gcgtgcggat ctccggcgtg gcggggaggt cggggtgcgg cgcgatcacg cccgcgttcc
     1561 gcaccagggt gtccttgaac tgtgcggcca cgccttgctc gatcgcgagc acgccgtcgc
     1621 ccgcgagcgg gtcggcacgg tactgggcat cgaagccgcc gatgccgccc gcgccgacga
     1681 tgccccgggg cgctgcaggg taggggccgg ccgtcgccca ttgcgccgac gcgtcgagct
     1741 ctcgacggca gcccgccatc ggatcgacgg gtgcgcggct gtcaccgaag agcgtgtccc
     1801 agaaatcgcg ctgcagcttg ggcgcggcac tctgcgggcc gccggcatcg gcctgctggc
     1861 cgacgtgggc gagctcgtgg gcgagcagct cgaggccggt gtcgctcgca ggggcgtagc
     1921 tgccggcgca aaaatagacg tcgttgccga ccgtgaaggc gcgtgcctgc atgctggcgc
     1981 acatcgagtc ggcttcggcg tcggtgtgga tccgcaccgc ggacaggtcg cgaccgagtc
     2041 gggtctcgag ctgtgcccgc aggccggggt cgaggggcgc gccctggccg cggcgtgcgg
     2101 cgatgcgttg ttcggtcgcg acgggcacgt gatgggggcc cgcgtccgca cccttcgtgg
     2161 cggaagccgg ggcagtcccc gcagcgctcc ctggccgggt ctcgcgcatc agcctggggg
     2221 cgagggtcgg ctttcgcggt ggggtgatcg ccggttcgag gctcccgtgc ggagcctcgc
     2281 cgctgcagtc cctgcactcc cgcccggaag cgctgcgggc cggcgtgcgc agggcgcggg
     2341 agacctcgcc cgcgatgcgg tcggcctcgc gctcctcggc gtcctgcggt tggctgacgg
     2401 cgagccgggc ctgcaggtag ccgggcgcac ccgggcgttt gccggcggcg ctgccgccgg
     2461 gccgggcttg cggggtccgc ccggtcacgg cctcgccggc gtggtcctgt cggctggcag
     2521 gagcggtggc cgcggacgcg ggggggttgt ccggcgactt gtgggcagga gccgtcttgt
     2581 ctgccggcgc gaggcgttgc gcggtggcgg cggggtggtt catggcgcac tccgtgcgtt
     2641 caggagctgg ggtggaggtg tgcggccgat cttgcggtac tcctcgagca gcgccgccac
     2701 cagccgttcc gcggcgatcg ggcgggccgg ggcgccggcg cccgcgtccc cgaccggatc
     2761 gagcgcggcc gcctgcagca cggcgttgcg gatgtagccg ccggggagcg tgcaatagct
     2821 ggcaagctgg cggcaaagcg cggcgtcggg ggcgcgtcct ccgagatgcg actcccagag
     2881 gcgcaggcgt tcgttcgcgc ccggcggagg aaaatcgatg atcacgtcga agcgacgggt
     2941 gaaggcgctg tcgatgcggc cgcgattgtt gctcgtcagc acgacgatgc ctgggtgctg
     3001 ttcaatgcgc gtgaggagga agttggtgag catgttggcg taacgctcgc cggtctcctt
     3061 gccctcgctg cggcggccga agagggcgtc cgcttcatcg atcagcagga tcacgtccag
     3121 agccgcggct tcgtccagca ggcgtccgag gttcttctcg gactctccga tgtacttgtt
     3181 catcaccgcg gacaggtcca cgcggaagag cggtgcgccg aggatggtcg ccagccggct
     3241 tgcggcgagc gtcttgccgg ctccgctctc gccgcagaac agcgcccgca cgccgcgtgc
     3301 cggctcggca agactgctgc cgaggccgtc cgagagcgtt tcgcgctggc ggcagcgcgc
     3361 gaccagggcc gcgaaacgct cgagcaggtc gtcgggcagc accagcgcat cgtggggcac
     3421 ttcgcgtgcc accggctgcg cgagctggcg caggcgctcg ctgccgaagc cggcgcgcgc
     3481 ccggcgcagg tggccgaggc cgggcgcctc gccgcgcacc ctggcgtcga ggcgcatgcg
     3541 cccggcgagg gtgcgcacgc tcgggccgtc aaggagcgca tgctcggcga agctgcacag
     3601 gcggtcctcg tcgaggcagg ggctgccgtc cccgtcctcg ctggcgaggg cttcgcgcca
     3661 ggcctcgcgg cgttcctggg cgctgagcgg gggaaggtcg atctcgatca gtgcggacgc
     3721 ctcgatcgcg ccgtcgcggc cgccgaccag caacatgggg acggcgagcg cgggatgctc
     3781 gggaaggacg tggcgttcgc cgggcccgag ctcgagcgtc agcaccggca gccagcctgc
     3841 atagcggcag gcggcggcga gggcgggctg gcgtgtccag gtcgccgcct ccatttgcag
     3901 cggcgggcgt cccgccgccg ctgccaggcg cgcggcggcg gcaagtccgg ccgcgcgtgc
     3961 gccgcggaac atcacgtggc gggtggcgcc gctgcgcagc atggtcgcga gcgtggggat
     4021 ctgttcgcgg aagccgcgcg gcagcatggt gtcgccatcg gcgagcggcg cggtgtcgcg
     4081 ccacgcggtg gggtcgccgc agagcagcgc ccacagctcg ggggcaaccg ccaggctgcg
     4141 ggtgggcatc ggcccctcgc ccacgatctc gaggaccccc gcccgcacga ggcggtggtt
     4201 gggcagcgcg aggggcgcga gcggagcgcc gaacaaggcc gcgctcgcgg cggagaggag
     4261 atgaaggccg gggcggagcg caccgggcga ctgcaactcg gcgagcgcca ggttgagcag
     4321 gtagctcgac tcgttctcgc cgcacagcgc catggcgaac cactcgtgca gctgcagctt
     4381 caggtcggca agcaggcgcc cgagggggcc gtgcagctcc ggcgcggctt gcgcgagtgc
     4441 gatgcaggcg gctcgccagt cctgaggcgg ggtaccgagc agggtggcga ggtcttcgcc
     4501 cccctggctc gccgcaagcg cctcgaagac cgcggacggg gcgccgacgc tgaggcgggc
     4561 gaactcggcg atgccggcat ggacgaaggc gcgtgacagg cgcgcgccct cgctcgccgg
     4621 ggcggattcc gggcgcaggc tggcgggggc gttcatggtg aggatcctcc gcgatcggcg
     4681 gcatcgctgc ggtcgtaatg aaagcgcacc acccgcccta gccagggcag ccagccaggg
     4741 tcgaggtcga ggccgccccg acgcagctcc aggtcgacct cggcgaggcg caggtggacg
     4801 tcgacatggc tcggacttgc gcgcacgcga gcggggcgct cggcgcgcag cgcgcgcagg
     4861 gccgcgtcac cgacgtgacg cagcacggcc cggtcgaccg cgacgggatc ggcgcgcgcg
     4921 ggcagcttcg caaggcccgc aagcttggcg acggggtctt gctcgccctc cagtgcgcag
     4981 gcctcggcca ggaagccggt gagcggggcg tcgggagcga gctcgagccg gagcgcgaga
     5041 cggacgagtt cgcgccagcc ggcgtggggt tcgtgcaggg tgccccgcca ggcgcgcaat
     5101 cccggatggt tcagcacgtt gagcaggaaa aaaagcccgc cgcggccggt gtggaagtcg
     5161 acctcggcct gcgtcgccgt caccgtcacc gcgaacgggt cggtgacttc gctcggggct
     5221 cctgcccggg gcggagcggc ggggcgcgcg tttaccgcag cgatggcgcg gggagaggct
     5281 ccgacgcgga tggaagcggg gggtgcgaac cctcgggagg cgggggagcc ggcgggtatc
     5341 gaacctgcac cggcgtcgcg ccgccgtctg gcgtcgggta ggtccccgcc tgcagtgcgc
     5401 gcgcgaccag gtccggatgc ccgtgcggtc gccgactccg catcctgccc gccgccgagt
     5461 tccgccaggg ctgcggccgc gccgcgcagg acctcgcccc cctgcggtcc cgccagcgct
     5521 gcgggggtac agcgccacaa ggtgagcagg gcctccagca cgacgagcgg atcgctggcg
     5581 ggcagcgtgc catgcgccgc cgcgccgggc ggggctggcc ggcgcggcga acccgccctt
     5641 gtcgccgccg gcccggtggg cgggggcgac gggccgggct cggggcctgg gcggatttcg
     5701 tctccggata tgcgctcatg gtgggcttgg gcctccgtca tgaggtccgc gccgctccct
     5761 gccgcctccg cggcacgaga agcgagcgcg tccgagatgg cgccgtgcca gccggtcgcg
     5821 gccccgatcg cgcgcaggag gctgcgtgcc gcggcggcat cgagacggca ccacagggtc
     5881 ggcaggcacg cgcgcagcgg cgggtgctcg agcagcgcgg ggagtgcgag ggcatcctcg
     5941 ccgagcagct cggcaagcgc ggtcgcggtg gggtgggcga gaagggccgc tcgatgacgc
     6001 catagccagc ggcgtgcggc ggtgccggcg aggaggtcgc gaacgaggca ggcgcggtag
     6061 tcgcgtgcgt cgcgaaagcg caccacctcg gcgccatcgg cgcccggagt ccacggatcg
     6121 accgcccggc cggcaagctg tccggcaagc gcagcggcgc gcgcgggcag ctcggtgcca
     6181 gtgccgccga cggcgatgcg ccgcacgacg aggatcgctt cctcgtcggg ccagcgtgcg
     6241 cgggcgagtg cgggcggcag ggtcgcgcgt gcgtgcgcga tcgccgtggc gctgccgcgc
     6301 agcgtcaggc gaccgatgtg gaggtcggcg agcgcgatca tcgggctcag cctcgatcga
     6361 ctccgctgat gtgcagtccc gtggcgagca cggcgccggg gttgcgttcg agctcggggt
     6421 cgaggatggc ggcgaccgag gcgacggtgc cctggtcgtc atggtcgtcg acacgcaggg
     6481 tcgcgatcac gcgctggtcg tcgtcgaaga gctggagcag ggtgccgggc gcggcctcgc
     6541 ggtcgagctt caggcgcagc gcctcgccga ggcggaagcg ccccgggatg ctctgcaggc
     6601 ggccctgcac gagctgttgc gggtcgtcgc tggccgcgag cagcaggcag gcgcgcacca
     6661 ggttgcgcat cgcgccgtgc gcggcggcgg aggcgtcggc atcgagctgg cggaaccacc
     6721 aggcgacgag ctcgaccagc gtgcgcacgc cgttgaagtc gcgctcctcc gcctcggcga
     6781 ggccgggcca gcgctcgggg gcctcgacgg cgagccggtc agcctcggcc gcgctcgccc
     6841 agttcagccg gatcgaggga gagatcgtgc gcagacgctc gagcaggcag ccggcggcgg
     6901 tatccaggcg ctggtgcagg gcacgcgccg ggtcgcgcag ctgtggcgag gggatcgcga
     6961 gcaggtcgtc gagggcgagg atcgcgcctg cctggcgctg caactcgccg agtgcgccgg
     7021 cgctgaaggg gcgcgcggcg atgtcgcgca ccagggcctt gttctgctgc accaggccgg
     7081 acagcaaggc gaggccggcg tccggggggc ggttcaggcg cagtgcgagc ttctggcggc
     7141 gcatctcgac caggccggcg aggatcgtgc gccccggcag caggtggccg agtggccgca
     7201 ggtgtgccca atcggccgcc gggatgtcgt acaccgcctc gatgaaaggc gcgagcgcag
     7261 ccggcagcgc ggtgtcgcgt cctgcgcagc cgggcacgag ctcgtcgggg ctgctcgggc
     7321 gcagctcgag cgggtcgggc aggctgcggc cgagcggggt ctcgcgcacc ttcacgtaga
     7381 agaggccttg atgagcctcg atgattcgcc ggcgctcggc gtgggcggct tcgacctcgc
     7441 gccagtgttc ggccagcagg cgttgtgcaa ccgcgtagtc gtccagggtc tcggtgcgtt
     7501 cgcgctccag gacgtcgagc cggcgaagct cgccgggtag ctcggcctcg agtgcgacca
     7561 ggcgggtgcg caggtcctcg agcgcctcct gatggcggcg gcgcagcgtg gtcagggcgc
     7621 cgcgcagcgc ctcgacctgg cggatgtcgt cggcaacgac cttaccggca tggaagagcg
     7681 ccgattcgtc gttgtgcttg acgccttcga tgtcgtcctc gcccgggcgg ggaagggcga
     7741 ggtcgagtcg ggggcgcagc agcacgccct cggcgttgtg cagatcgggc gcgacgagca
     7801 ggcggaaggg acgtgcctgg gcctcgtcgt cgatgacgcc gagccggcgc agctcttccg
     7861 gcttgagata gtcgtcgagg aagtcgcaca cgcggtcgat gagcgatttc gcctgattct
     7921 ggcgagcgtt ggccctggcg cgcagctcga ggaggtcgcg gcgcacgttg aggaagctgt
     7981 cgtgcacccg ctcgacatgc gccagcattc ccctgccggt gctcgttgcc ttgctgaaga
     8041 ccggctcggt gagcggctcg tcgggcgcgg ccttcggctg caggtcggcg agggcggcca
     8101 gcatgccggc ggagatcgtg gcctcgcccc gggtcgcgtc ggcgagatcg accgcggctg
     8161 ccgtgccctt cgcgtccacg aacttgagtt cggtgaatgc gctcgcgtcg gtggtgaagc
     8221 gcttgaggat gtcgctcatg acgggttctc cgtggggggc ttgggcacga agttcgcctt
     8281 cgccgtccac ctgacgagct gcgaaccgag gccgtcgccc ggtacgccgc ccgcaagcgt
     8341 gctgaacgac accgtcagcg cgtcgagttg ctggcgctgc aggccgagga agtcgccgac
     8401 ctcctcgagc aggcggaagc tcttgtcgag cgcggcctcg aggtggcgga tgtcggcgcg
     8461 cagcgcctcg cgccggtgca gcaggccgtt gccggcgggc tggatgtcgg ggacgggctg
     8521 gtagtcgtcc ggggcggggt gggggtcggc gaggtggctg gcgtagggtt cgggcagcgc
     8581 tgccgtgccc tccgtggcga ggtccgcgag gggatgggac gtcgtgccct gcggagcggg
     8641 ccgcagcgtc ttcgcgcggt cggtgaccag cttcgtgcgg tattgcccgg gctcgggggg
     8701 agccttggtg agcagcaccg gcgcgcgcag cgcctccagc tgggcgccgc tcagaccgtg
     8761 gaagagcgca taccaccgcg ccttccacgc ttggtaggcg tgcgccgcgc gttcgccgcg
     8821 gcggaacagt tcgtcttcga gccgggtgtc gcgctgtggc aggtcgaaga ggtcgcggcg
     8881 gtagtcgaga tcggggattg ccagcaacag gcggagatgt tcgccgagcc cgtggatcag
     8941 gtcgacggga ccgcgcccga gctcgccgac gaccacctcc tccacggtgc ccgccggcac
     9001 cggcgcgagc tcgacgcgca ggtcgccagg caagaaggcg aggctcgggc tccgcgcggc
     9061 ggggtcgcgc agcagcgcgg ccgggagcgg gccggcagcg ggaaggaaat cgagcccgag
     9121 gagttcgtcg agcgtctgcg tcgccaccgc ggggaggggg gcgggatgcg cgcgctgcca
     9181 ggtctcgagc gtggcgacgg catgggcgag caggctatgg gcggccgcat ccggctcggc
     9241 gagcaggcgt ccggcgcacg cgtcgaacca gaccggcagc ttgccgacga tcttcgcgag
     9301 tccgagcggc acgccccccg agtccgcgcg gaagggcgat tcgcgcagca ggcggacgcc
     9361 gatgcggttg gcggcgcgcg cctgcggcat cgccatcatg cgcgggctgc ccgcgatgcg
     9421 ctgcaggccc agcaggctga ccgtctcgat gcggcgctcg cgcagcggat ccggttcgct
     9481 gcgggtgcag ggttccgctg cggtcgcatc gtcgatctcc tcgaccgcgc tgcggatggt
     9541 gagcaggaag aggccgtcgc gcagcggggc gtcctggtcg cgctcggcct gggcgacgag
     9601 ctcgctccat gcctggtcga gcgggtagaa caggcgtacg agctggccgg ccgcgccgac
     9661 ggcgaggcca ggcccgacgc gtacgcgggt gtcggtgccg gtgccgaaga gcgcgcacgc
     9721 caggccctcg acgatgcctg gcgcgaggct cgccagcaac ggtgcgatgc gatcgtcgac
     9781 gtaggcctgc aggcgctcga actcgcgctc gccgagcgcc cgcccgggga agaggttgat
     9841 ccggcgcggg tcgtccgggc gtcgctgcgg ctcgtgcagg cggatggacg gtggcgtgcg
     9901 gggcagcagg gccgcggccg gtcgcgacgc gagcgcggcg aggaggccgg gcgggatgaa
     9961 ggacagggtg gccatgattg ctcaacgcct cgtgtgggtt cgggatgcgg cgcccggagt
    10021 gtccatggtc gtcagccgag gacgcgtttc cagagttcga ggagcgcacc ggaaagaccg
    10081 aggccggggc cttcctcgag gaagaacttc gcaagatcga gctgaccggc cgcggcgagg
    10141 aagtggtcgc gaagcttcac gaagacggcg gggtcgagtc gcgccagcgc tggcagggtc
    10201 ggccacagcg cggggtcgag gcggcggtcc gccatgccca cgatctgcac tgcggcgatg
    10261 cgcgcccgcg cgtcggcgcc gatcagcttg tcggcgccgg tcgcggcctt gatgccggcg
    10321 tcgtccgcgc cgcgggcctg tgcgagcagc gcgaggggaa gctgggtggc gaggtcgccg
    10381 gcttgcgcag cctcgcgctc gagcctgccg atggtggcct ccgcgaccgc gagccgggtg
    10441 gagagcgccg tgcgttcctg ttccagtgcg gccgcccggg tgcgctcgct gccgagtgcg
    10501 gcgacgagct cctcgaggcg cgcctcgagg gtctcatcga ccggggcgct ggcggtcttc
    10561 aggcgctcga tctcggcatt ggcggcgtcg agcgcgagct tcagcgcgtc gatccggtcg
    10621 ctcgccgcct cgagcgcgtt ggcgaggatg gcggcgttgc ggtcttcggc ctcgagcgcg
    10681 gcgatctcct tgcgggccgt ctcgagcgcg agcccgaggc gctccacctc cgcgagcagg
    10741 tcgtcaggta cgccggtgcc ggttgcggcg agggccttct cgagctttgc gatgcgcagc
    10801 ctcaggcgcg ccgcctcgtt ctcgagcgtg gtgcgcgcct gcgcggcggc gtcgaccgct
    10861 tcgagggccg catcgagccg ttcctcgagt gctgcggcgt cgggcggcag cgcgctgccg
    10921 ggcggcggca ggctcgcgcc gagggcgagc accagcgcgc tcaccccggt ttccgcggtg
    10981 cgcggcgggc ggcgtacgta catcagctcg gccggatccg cttcggccca gatcgcgcgc
    11041 cacacgtccg cgtcggtgtc cacgcggtgg atcggcgggc gcgggaggat gcgcagggcg
    11101 aggcggtcga ggttggcgag ccgcccgagg gcgcgcgggc ggaactcggc cggtgcctcg
    11161 agctggcggg cgcgcagcgc gaaggcgctg tcgctcatcg gcacgagcac catcacgtcg
    11221 cagtcggcgt cgcgttcgag gtcgatcggt gcgagccggg cggagtcggc gagcacggcg
    11281 gcgagctcgc tgcgccgcac cggggcgatg gcgacctcgt aatcctgcgg gaagaactgc
    11341 tggcggccgc cgaccgggtc gatcgcttcc tgcggcagcg gcccccaggg ggggagcacg
    11401 cggaaatact ggccggccgc gaaggctccg cgcaggccgg cgctgcggcg cgccgcgagc
    11461 acggcctgca tcagctcctg ataatgcgtg gcgaggtcct gctggatcgc gtcgggggtg
    11521 ttcggcgggc gctgcggccg gcgcagcagg ccgcggtcga gccacaggat ccgtccgcgc
    11581 tcgaccgcga tgaggccgag cgcgacggcg tcgtccgagg gcagcgcgag gcgctcgcca
    11641 ccggagagca gctcgcgcgc gaggctggcg cgcacggcga tctcgtcctg gcgtggcagc
    11701 ggcagcggca gcggcatcag gcgcagctcg acgccctcgg cccatgccgc gacatgagga
    11761 acccgcgcgg tggagaggtc cttggggaag gcctcggcga cgccatcgac gacctgggtg
    11821 tgggtcagcg cgagggcgtg taggccgcgt gcggggccgc ggaagcggcc gtcgttgagg
    11881 gtcgcgatca ggccgctgtc gagcaggtcg atcctcagcg cggtgttcga gagttgcagc
    11941 acgcggcccg agggcgcgat cgcgatcccg ggagagactt ccagcaggtg gcccgaatgc
    12001 aggctgggtt cgaggccgcg cacgatgccg gcgccgaaga cctggccgag ctcgagcagg
    12061 cgttcgtcga ggtagatctg gtcgcgtgcg aggtcggccg ccttcaggag ctggccgtcg
    12121 aagtagttcg tgcgcgacag caggggatcg acgctggaga tgcgctcgcc ggcggcgttg
    12181 ctcgtgccga tccggagcgg ggtgaagtgg atgttggcca tggcggttcc ttaggcgcgg
    12241 atcaggcgag caagcgcgac ggcgttgaag aggaaggggc gcacgaggtt gtcgatctcg
    12301 attcggcgcg gattgacgat caggtcggga tcgggcgcgg gcggcgcgag gcggatcgca
    12361 atgcgcgcga gcagggtgcg agcgagtgcg gccgggtcgg tcaggaaggc ctggcgtgcg
    12421 accagggccc ggttgtcgtc gcctagcgaa tgcagcaggc gggcgccgag ctcgagctgc
    12481 gcgcgcgcgc cgccggtggc ggcctcgacc tgggcgcgtt cgcgctcgcc cagcttgtcc
    12541 tcgatctcgt cgtaggggcg gatgcgtgcg tgggcggcaa agaggtgctc ctcggccgga
    12601 gcgtcgtcgg ggcgctcggc gacgagctcg aacagcacgc agtcggcgac gcggctcggc
    12661 tgcaccggat cggtgcccgc gttgagctcg gttgcgagca ccggctgcag gccgctcgcg
    12721 cagtcctgat agcgcatcgt gaccttgagc cacagcaggt tgtcgtctgc ggcgtagccg
    12781 tcgcggatca gcgcgttcca gcgctcgggc tcctcgtgct gggtggtgag ccaggcgccg
    12841 agatcgacgc agtagggctc ggccacgctc agctcgcgtc cgaggccgtc gacgccgatc
    12901 ccggggctca ccaccaggcg cacgcgcacg ttttcggtgt cgttgccggg atccttggac
    12961 tgcaggctca cgcgcagacc cgcgacggtg ccggcgccat gcagccagta gccgtggcgg
    13021 gtggcgcggc ggcggtggta atcctgttcg gcaagcgtcg cctcgatgcc gagcagcatg
    13081 ccgggtgcgt agttcacgcg cttcagcatt tcctcgaagc ggccgtcgct ggcgaggctc
    13141 gtgtcgcgct tgtcgtcggg ctcgctcatg gcccttctcc tgtgttgtcc ttcacggtgc
    13201 gtccaggacg gtcgcgtcga tcggcacggc gtgctcgggg tcgagggcga ccgggttgtg
    13261 cagcaggtca ccgcgaccca gccgtgtgcg gtcgagcacc acgggccgcg ctggtggcga
    13321 ggcttccagc caggtgtcga tgcccagcag cggcgacaga cccggcacga aggggtgctc
    13381 gctgctgcgg atcgcccatt gcacggtcgc gggcacgagc gcgggcaggg cgtcgcgcac
    13441 gacggccgcg agccgtcgcg ccggtccgtg caggatcacc gtcatgcgcc gcgcggcttc
    13501 ttcgaagaag cgttcgacca ccgcggcctc gcccttcgct tcggcgacct ccggggcgaa
    13561 gagggcgagg aactcgcggg cgaggtcgtc ggacaggatc aggctgtcac cgacgaggct
    13621 gttgccggaa agaccggtgc ccagggtcag cgggtggtcg cggtcatcca tgtccacacc
    13681 gaggatcgtt gccatcgtgc ggcgcaggcg gaagtgctcg accgggatca ccgcgccccg
    13741 cgccaccgct ccatcggtga ggatgtcgag cgcgagcagc aggccgcggt agctgccgtg
    13801 gctctgctga agcatgccct gcgcgcccag ccagcgccgg cggcgtgctt ccggccagtg
    13861 gggcggcagc gtggtgccga gcatcgcggc gaggccgggc aggtgcgcca cgggcgcgac
    13921 cgcggggtcg agcaggatct cggctgcggc gatgcggtcc tcgatcgggg tcagcagacc
    13981 ctcgaaactc gccagcagac gctcgcggaa gtcggcgccg ttcgcctctg cctggttggc
    14041 ctccgcgcta gcgagcggac gctcctgctg ctggaaatgc tcggggaggt agtgggtctg
    14101 ccaggagaag cgcgggtact gcacgcgcag cgcgaagatc gccgggctgt ggcggccgtc
    14161 gccgtgcatc gtgatgcgca ggcgcagata gcgcccgcgc acctcgcgca ccgcgccgct
    14221 ggcgcgctgg atcagcagct cgaacaggcc ggaacgatct gcgtgatccc cactcccggg
    14281 tgcgcgcccg gacgcgaagg gtagctccga cgacaccgcg gtcagcaccg gctgcggctg
    14341 cgcgatccac tccgcgggca ggttctcgcg gtcgtcgccc gcctgcacgg cgaaatcgat
    14401 ccggcagccg ggggggatgc aggcgtcgat gaagatgcgg tcccacagcg tgtcgggcat
    14461 cgccgagtcg agcggcgtgc ccagcgtgac cgtgccgttg ggcgcgaagc gggcctgcgc
    14521 gaggcggtag agccggaccg ggccgtctgc gctcagggcg cgcggccggc cgtcgcggtg
    14581 tcgcacgaag cggtccgcgg caggcagcgc tgcggcggga cggcgcggcc agcgctcggg
    14641 caccagctct gcggcgggcg cgtcctcgcg catggagatg agcgggcagt cgcgccgcgt
    14701 tccaggttcc gcgccttcgt cgatgggcgg caggagcagg agccggccct cgccggccgc
    14761 ggcgaggtcg gtcgcgagcg gcagcccgtc cggcaggcgc cggatgtcga agccctcgcc
    14821 cggtgcggcg ccgagcgcac gcatgaagat ctgcatgcgc ggggcgtccg ccgtgctgtc
    14881 gggggtttcg ccgagcacga agagatggtc ttcgtcggtg cacagcccga gcagcccgcc
    14941 gtggggcggc agggccagcg tccacgccag gcgcagcgga tcgggattgg tctgcagcgg
    15001 ctcgaagcgc tcggggcggc cgcggtaggg ctggggcagg ggcgccccca cggcgaggcc
    15061 cagctgcgtc tcgccgagca gccacacgcg gtcggtgccg ctggcggcct cgacccaggc
    15121 gcggcgcggc gcgaagggca gcgcgcagcg ggcctgccag cgccggcgca gatgcaccgc
    15181 ggtgagacca tgctgtacgc caccgtcgct ccacggcagc acgaccaggc cgctgccgcc
    15241 gaaggcgagg tcggtgaagc ggcccgccgg cgcatcgacc ggatcgagca ccagcacctc
    15301 gagggcggag gcatcggcgt gcgtttcgtc gagcgtggcg cgcaccggct gccaggtgcg
    15361 tggcggccag gacggcgcgc actccaggcg caggccgtcg tccgacaggc gaccgagctg
    15421 gccgtggtcg tcgagcatcc atggcgtcgc cgcgagcagg cgctcgcgtg cgcgcgcggc
    15481 cgccaggcgt ggcaggcgcg gggcgtcctg gcgggtcagc gtgaaggcgc gctgctcgtg
    15541 gtcccagctg cattgccggc tcgtgttgtg gaagtcggcc gcgccgtcga ggagcaggaa
    15601 gcgggtgccg ttgctgttca tggacgtggc ctcgatcagc acagatccgg aatcaccggt
    15661 accggcaccg ggcgcgggcc gtccgctgcc gcgcccgcct cgggtccgaa gccgcgcggc
    15721 ggggcggggc ggtcctcgcc ggcgcggatg gagaccgcca tcagctcggg gagttcgaag
    15781 tcgagcagcg taagtgcctg cacaccggtg agctcgcgcc agctcggcgg gtcctgctcc
    15841 gtcgtccggt agaagaggcg cagcgcattg accgcctcca cgccctcgac gcgcccggcc
    15901 tgggtgcgca gctcgttgat ctcgacggcc ttgccgcgtg gccagcccgc accgcgcggc
    15961 ccacccggcg gcaaggccca caggtagcgc agcagggcgc gctcgacctc cgcatggacc
    16021 tgctgctcgc tcgtcggatc gaccacctcg agcgagagtg cgagcgcgat cggtacgaag
    16081 cgtggcgaaa gcacgtagag ctcggtgccg agcatgcagc gcgccgagag gtacgcgtgc
    16141 acgtccgcga gcagcccggc gttcggacgc ggtgcggccg ccatcgcctg cggtgccggc
    16201 ggcatcacga acacgctgac gacgccgggg acgttgcgcc gcaccgcggc gaggcttgcg
    16261 ccggggaaga atcctgccac ggcctcggcg cgcgcgaccg ggttgatcgg gttgtcgcgc
    16321 gcgagcacgg cgaagtcctc cgccgtgact gcccggtcgc ggtgggacag gaaggccggg
    16381 atgcggcgct cggcttcgtc cagcgactcg gcgtcgacgc cgccgcgggt cggccactcg
    16441 tggcgtaccg cgagcttttc gctcgcgcca tggagttccc ggatgctgcc gggtggaagg
    16501 tttccgccgc tgccgccgcc gtggcggtag aaggcggcgc ggatgcgggc cccggcgggc
    16561 ggacgcatgc cgcggacgcc gtcgccgaag tgcacgctgc ggtcggcgga gtcgacgacg
    16621 aagacggtgt cgtcgggacc gctgccggcg aaatgggcga cccgggtcca ggcgcgaaag
    16681 gcgcgtcggg cctccacctc gatctcgacg ctgcgctcat ccacgtccgg gtcgggcagt
    16741 tcgaggcgct ggtcgggacg gccggtgccg gcgccgagca ggacgtcgcg cgcgatgccc
    16801 tggccgatca cctcgaccgc gttgaggccg aggtagccga gcgtgagatc gggctcctcc
    16861 gcgcaggaca ggcgcagcca gaacagaagc tgatcggggc gcaggtcggc gggtgcctcg
    16921 ggcggcgtgt cgccgagtcc ggcgaactgg ggatcgaggg cgctcgtcgc gcgcagcatc
    16981 tccgcgtcgc gcggcaggcg gagacgtgcg acgccggtcc tgcgtccgcc cgccgaactg
    17041 tcggcgacga cttcgagcgg gaggtactgc accctttgcg gtgcatcttc ggggtcgggc
    17101 caccatgcca gctcccacgt cagcctgcgc gcacgcaggc cggtggcgag cccgccctcc
    17161 agctccgtgt ccggcgccag cccgacgttc aggaggacgc caccgagcgc ccggcgcacg
    17221 cgttcggcct gcgtcgccgg ggctggtctg gcgaggcaga tgcccagatg cagtgcgccg
    17281 tcgcaggtcc cggctgcgct cggccggtcg cgcccggggt gcagcgtcac cggccggaag
    17341 gcggccggct ctaccccgta ctgcgcgcgc aactggtcga ggctgatgcc ctcggcggca
    17401 agcgcggcct cgtcgagcct gcgcttgacg acgatgcgca gggtgagtgg cgtgggctgc
    17461 acctcgccaa tggtcgagaa actcgtcgtc cccgccttga gcagcgcctc gggtggaatc
    17521 ggcagcggca gcgcgtgcgc ggtcgagtcg atgctgacga tgccgctcgc cgggcgtgcc
    17581 gggcgcaggg gaatctgcag caggttgagg aaggccaggc gctggcggtc cggaatgcgg
    17641 ttggcgcgat aaaggatggt ttcggtgagc caggcgaaca gatccaccag cgcgaacacc
    17701 gggtcgccgg gcgcgagcgg ggcgagttcc ggcacgtggc gcgcgaggcg tgcctgcagt
    17761 tcggcgacga gttcgtcgaa gcggcggtcg tcaaggtcgg ggacggggaa cggcttgggg
    17821 ggcgacattt gttcggttcc ctcaggattc gggtgcgcgg cgggcgtggg cgaaggcagc
    17881 acccttcgcc tggcggcgtc gagacggggt gggggcgatc gaccggcgtg cggcgtgcat
    17941 ggcagtcatc ccaggtccag cgccagttgc agcgtctcgc gccgctcgtc ggcaagcagc
    18001 cggtagcgca cctcgaggtt cacctgcgac aggcgctcgg ggtcggccac ggcgcgcacc
    18061 gcctcgatgc tcacgcgcgg ttcccagcgc cgcagcgcgc gcagcgcggc gtcggcgatc
    18121 agcgcgcgcg tggtctcgtt gttggggtgg tggatgaagc tgcggatgcc ggcgccgaac
    18181 tcgggccgca tcaggcgctc gccggggcgg gtcagcagga tgttgagcat cacttcgcgc
    18241 acgctcgcgt tgccggtctg ccagtgctgg cggccaccag cgaccgggct ggcgaggagg
    18301 gggaagctcg tgagcaggga ctccggggtc ggcatcgcgg tctcctcgtt ggtctcactt
    18361 gccccggagc gccttcaggc acaggcgcgg cagcgcgagg aatacccgcg gcagccagcc
    18421 gaggaagagg ttcagcaggt tgatcacgac catcagcagc atggtcgcgc acagcgtgat
    18481 cgccggcagg ctgaagctga agctccagca cgcgccgctg cggttgcccg ggccgtcgcc
    18541 cttcatgtcg gcgtccggac tgatcttgcg cagcacgtcg gcgagcgact tgggcgccag
    18601 catcgccacg ccgcgcggaa gcccgcgctt gaggtcgtcg agcgcgggca ggaccaccgt
    18661 ggtcggacgc tgcgcctcgg ggtcgaaggg cgaggcgacg cggaagacgt ggctggccgg
    18721 gccccaggcg acgcgctcgc aaccgcactc gtcgtgccag cggatgaagg gcagggcggt
    18781 gaagcggtcg tcgtcgccct ggccgtagcg cggcatgggc aggccgtcgt cgaagcgcac
    18841 ctgggcgcgc gccaggcgca gcccgaccag gctgcgcagg ttctcggcct gctgttcgga
    18901 gagcagcagg cggtccttgc ggcttccgcc cgtgccgtcg ggcagcgcgg tgcggccggc
    18961 gacgaactcg ccgcggtcga tgcgggcgaa gaactcgacc agggccgccg cgctgcggtc
    19021 cagccattcg agtgccgtgt cgccgaacac cggcacccgt tgcgggaagc cttcgccctc
    19081 gacgaggcgg acctcgaagt ggtggaagcg catctggccg agcagggtgc ggaggccggc
    19141 gttgtcgggg tcggtggcgt cgtcgatgcg gaactgcgcg accagggtct gcagcagctc
    19201 gacgaagccg acgctggcga caccctcggt gacgacgagg ccgtcgccgt ccacccaggg
    19261 cctggcgtcg cgggtgccga gcggccatgc atgctccagc ccgaagtcgg gggcgtcgcc
    19321 gccgtcggtc tgcggcagcg gcgcctcggc ctgcacgcgg gcgctgcccg agagcggcag
    19381 gtagccccac agcagggtgt gcttgcgctc gactccgcgt gcgtcgcggc gggtcgccag
    19441 cagcgtgtgc aggggggcga cctcctctcc ggaatagggc ggttcgggga agcgcaccgg
    19501 cagcagcccg cgcgcgcgga ggcggtgcac gtcgtcgggg tcgtgatcgg ggatcctgcc
    19561 gtcgcgccag cccaatggct ggccgtcggc gaccatccag cgctgcgccg agccgtcggc
    19621 cgctacccgg cgcaccacga ggccggcgcc gaggatcttg cgcggatcga aggcggggcc
    19681 gcctgcggcc atgcagcgca cctcggcgca cacgaggtag aagcagcggt gcaggggcag
    19741 gcgcagggtc ggctcggcgc cgaagcggcc gaaacggtcg gcgcggaacc atggctgcgc
    19801 ggcagtcccg gtgagccggc cagcctgggc gtcgttgagg aaacgcgcga cgaagtcctc
    19861 gtcgaagtag cgatgcaggg tcagcccctc gccttcgccg gcgaagaagg gggcgatgac
    19921 ctgcggctgg tggggagccg cggagggggc gccgcgggcg ggacgcatcg ggcgctcggc
    19981 gcgcagggcg gaatcgatgc tcaccatagg ttccccgctc ccggcgaata gctcgtcgac
    20041 accaccgcgt tgctgatcag cgtgtcgcac tgcacgacgc cggagaactt gctcaggccg
    20101 gcgtcgacct tgaccaggcc ggcggagatc gtgacctggg cggcctcgat gctgacgatc
    20161 cccgagccgg tgagcttgac cgtgcccggc gcgatgtcca cccgctcgcc gccctgttcg
    20221 atcgtgacct tgccaccgcc ctggtcggtg atcacgaggc ggtttccggc cggggtctcg
    20281 atcgtcacgc tcggctgccg gtcgtcgagc agaaccttca ggccggcagg gctctgcacg
    20341 aggtagcgtt cgtcgaccgg gcgcgcgtcc tcgggctggc tggcgccacc gctccacacc
    20401 gcgccgagca cgatcggcag gtcgggagcg acgaaggcga ccacgacctg ttcgcccacg
    20461 cgtggcagga ggctgacgcc gtagccgccg cccgcgctcg gcaccatcac ggcggcccag
    20521 gtctccaggc cggtcgccgc caggtgcagc ttcacgcgac cgcggtcttc cgggtcgtcg
    20581 ttgtcgagga cgacgccgag gtgcagttcc tgcagcggac cggggctatg gcgcgacatg
    20641 gcgggcgctt cctcaggggg tccagtccgg ccgttcgacg cgcagtcgtg tcgacaggcc
    20701 ctgggtgttg tcgaagtgat gccagcactc ggcgatgcgg taacgtccgg cgagccgcgg
    20761 cgataccccg gtgagttcga gctcgcgccc gactcccagc tcgggcgtgc cggtgcagac
    20821 gatctcgccg tggaggaagc gtcgtgcatc gcggcggaac gcgccacgcg cgagcgattc
    20881 ggcctcggcc tgggtgcgcg ggaaggggtg ggggcgtggc gagcgcccct cccagccgag
    20941 ctcggccagc gtcgtcgcgg cgctgcgtcc ggggggggcg ggcgcgagtg cggcgctgct
    21001 gccctgggtg ctggagtcgg tggcgaggtt gtagccgtgt gcgagcactt cccgtggctg
    21061 ctggttgagg tcggcgatga tgcggatgcg gcgcgcgttc gctccggcgt cgatgcggac
    21121 cgggtccggg tccgcctcct cggcgcgcgc gtgcaggcgc ccgtcctgga tgcgcagcgg
    21181 gatgtcgtgc gggcccagca cgcgcagcag gaaggcgagg tcgctttcgt tgtgctgcag
    21241 ccagtccgca ctgccgacct cgatgcctac gtcggcctgc agtccggcgt cgtcggcgat
    21301 cgcacggacg accgcggcca ggccacggtt ctcgaacacc cggctggcgc gccggcgcgc
    21361 cagccggtgc agcgcatcct cggcgaggat cacgagttgc ggcgcgccgt cgccgtaacg
    21421 ctcctccagc gcggtgatct cgccaacgaa gacggcgccg ctggcgtgct ccccgaggag
    21481 gatctcgatg ctgctgccca ggcgcagatc ctggaacgcg aagtccggtg ccgcgccttc
    21541 ccccgcggcc caattgacca cgcgcgcctc ggcgctcgcc atgccgctcg cgggcagatg
    21601 caccgagagc gcctgcaccg cggtgccgag cgcggggcgg tcgtctccat cgacgcgcat
    21661 gcgcggacgg acgctgagga gcgcggcgct agtcgatggc aaggcgagcc tcccgttcgc
    21721 cggccagggc aagcaggtcg agcagggtgt cgccgggcgg accggccggt gggcgcgcct
    21781 cggtggccga agcttgcgcg gggttcggct cggcctgcac ctcggcgacg agttcgtcga
    21841 tgatcagcgg catcaggtgc ctccagtcgg aatcagcaag gcgcccggaa cggtgaagcg
    21901 cgggttgtcg aggcggttga gttcggcgat gacgcgccag tcgcgcggat cgcggccggc
    21961 agactgcgcg gcactcgcgg catcacggcc ttcgccgccg gcagcgaagc cgggcatcgg
    22021 gcgtgcgtct gcacgcaccg tggggtcgag cgcgaactgg tagcggtcct ccttgaaggt
    22081 gagggccagg gtggcgcgca acgggatgcc ctcgggcgcg aagaagtcga gggtctccga
    22141 gtagctcgac agcattccgg tgaactggaa gctgccccac tggaaacggc agcgcccggg
    22201 ggcccgcggg cggttcgacc cggcgtcctg cggcttcatg aaggtttcgg cgatacgttt
    22261 ggtgagcgtc ctcacgtcgc tgtcggcggc gaccgtctcg cgattcgccc gggcgtcgga
    22321 gccgagcttc gcggccacgc tggtgtcgaa cacgagctcg accgcgaggg tcgattcgct
    22381 cttgtcgatg tattgcgccg ccgcgccgct gccgccgctg cctgcccggt tgtcggcctt
    22441 cagcgtgttc gacagggtca cgcgcagcgt cgcagggttg aactggacgt cgatgtgctt
    22501 cgattcgtcg gtctcgacct ggtcgccgtt catcgggatc agcttggcac gggcgatctg
    22561 cggggcaggc atgtcgtcgt ctccattcag ccgcgctcga gctccagccc ttcgtgtacg
    22621 agctgaagct cctcgatcgc gacctggctc gcggtggagg acaggtcggg gcccttgaag
    22681 cgggtcggca gggcgttcgt gatccgccag cgcagcagcg gcttgtcggg gtcctgcggg
    22741 tgatagacgt cgatcgtgcc ggacaggcgc aaggcatggt tagactggcg cgtgctcacg
    22801 tcgaaccagt tccacaggtc cgccacctcg gtgatgccgc gcttgaggat gacgggcgcg
    22861 aagctcgtgg ggccggcgag ctgcacctcg ccccagttgc gcccgccttc ccgcagggtc
    22921 ttgggcgcca tggtggcctc caggccgctg acctcgctga aggcgccgcg gcacagcagc
    22981 cggccgcctt cgccgtcgta gagcgcgacg cggaagcgga aggggatgaa ttcgccggcg
    23041 gagtcagcca tcgatggcct cgatgcgggt ttccgtcacg gccgcgtggc ggtcgtgcag
    23101 gaaggtcagg cgcaggtggt cgatcgggta ggccggcgcg atctcgatct cgaacgcgat
    23161 cgcggaatcg acggccggca attcgcggat gcggtaggcg tgctcgggct gcgcgccgcg
    23221 caaggcgccg gcctcgtgca ggcgcccgaa aaagccgcgc agggcgagct cgggccgcgg
    23281 gtcgcccgga tcggtgtcga acagcagccg ctcgcccagg gcctgcagct cgcgccgcag
    23341 ccagcccatg aagcgcatca cctcggccga acgccagtgc tccagcccgc gttgcgcctt
    23401 cgtcatgcgc tgcaggtcgg cgccgggcag gcagggcacg cataaggcct cgtcgtccac
    23461 ctgcagccgg cccgcgcgcg tcagcaccac ggtcgcgccg ggcgttgcgc gcagggcggt
    23521 ggccgcttgc tgcgtcggtg gcggccacgg cagcgccttg ccgggcagcg ggcgcccggc
    23581 gatcgagcgc caggcgccgt accggagcga gaccgcggcc tgcatgccgg cgatcagccc
    23641 gcaggccggt gccagcgggc ggtccgcgct gcgcaggtag ggccacagca gctgcagatg
    23701 gcgcagcttg ccggccagct cggcgtgcgg agcgtcgtcc gcttcgcccg cgggttccgc
    23761 gccggcgtcg ccgacgatgc gggcgacgtg ggcgaggaag ccgcgcgcgg gcgcgggcag
    23821 ctcgtcctgc ggctggtggt ccagcggcag ggtcagcagg cactgcacgt ccggacgcag
    23881 ccgcgccagc gtgcgcagga tgggcgggac gatgtcggcg ggcacgcggg gcgtttcagc
    23941 gggcggacgc tcgcccgcgc ggcggcgctc gcgatgggtg tcgtcgactt cggtgccgca
    24001 gggcaggaac gcgggctcgg ggttggccag gcgcacgcgc ggcaggtccg gcagggcgtg
    24061 cgggatcagc agccgctcga ggtcggggag cgcgaggatg ccggcgcgct ccaccagcag
    24121 cgcgcgttcg aaggcgccca gcccggcggg ctcggccagg ctcgcgtcgg ggcgtggcag
    24181 gaaggcggcg aagccctcgg cctcgggcac acggatcacc cacagcgcgc gcgtgtcctc
    24241 gaagccgcgc tcgtcggtgg cgaagaaatc ctgcaccgcc atcggcagcc acgccgcatc
    24301 gcccgccagg gtgctgcggt agccgcaagc gaccgcgaag gcctcgggga agagcacctg
    24361 gaagtggccg agcgagcgga tcgggatcgg cagccgatac agggcgagca aggcctcgtc
    24421 gccggcttcg cccgcggtgg cgcccgccgc gagggcgcgc aggcgctcga cctccgcgcc
    24481 gaagcccagc gccgcgatct cgccgagcac ggcggggtcg cgcgcgagcc tgtcccagcg
    24541 cgcacgcccg tcgagcgagg ggcaatggcc gatcagcacg acctcgaccg gcgccagccg
    24601 ccgcgccgtg ggacgtgcat cgagctcgat gcgcttgtcg gcgagcagca ccgcctcact
    24661 cctgctcgat ggtctcgacc gagaggacca gttcctcgat ggcgatgtcg ccgccgccct
    24721 tggcggtgag cgtcgggccg gtccacttga tcggcatcgc gccgcgcagc ttccagctca
    24781 ccacggtgcc ggcgcgatcc tcggattgca gcttgacgac gatgtcgcgc ttgacctggg
    24841 cgagcttgcc ggtgcgcccg agctcgagcc actcgtagag gttctgcgcg ccgatcacgc
    24901 cgcgcttgag ggtcacgtct cccgccttgt ggatgccggg taccttgcgc acgtaattca
    24961 cgcggtcgtt tccggcacgg tactcggcga ccgtgatctc ggcgttgagg cccgagacct
    25021 cggagaagcc ggcctgcacc tcttcgcctt gcccgggttc gaggtcgacg aggaagttga
    25081 atgccgaata cggggtttcg cgaaacacgg ccatgacgat ctctccttgt ctcaggcgtc
    25141 ggcggtcttc tgaccgatgc ggaagatgac gaattcggcg ggcttgagcg cggcgacgcc
    25201 gacgaggcac accatgcggc cgttgtcgat gtcgttctgg gtcatcgtcg agcggtcaca
    25261 gcgcacgaag aaggcctcct tcggcgtggc gcccagcagg cgaccgttgc gccattcggc
    25321 gaagaggaag tcctccacgc tggaacggat gttggcccac agtgcctcgc cgttcggctc
    25381 gaacaccgcc cactgggtgg atttctcgat cgagcgctcg agatagagga agtagcgccg
    25441 cacgttgacg tacttccact cgggatcgct ggagagcgtg cgtccgcccc acacgcggtg
    25501 gccgcgtccg gcgaaggagc gcaggcagtt gatgccgttc ggattgagga gctcctgctg
    25561 gaaggcgttg atctgctggt cgaagcgcag cgcacccatg acgatctcgt tcgcgggcgc
    25621 cttgtgcacg ccgcggtcga catcggtgcg cgcatacacg ccggcgatga agccggcggg
    25681 cggcaccgtg atgtcgcgcc gggtgcgggt cgggtcgggg atcaccaccc aggggtggta
    25741 gagcgcgagg cgggagtcgt cgaagttgcc ggcgaaggcg cgcagctcgc cgaggctctg
    25801 cccggcgcgc gcgtccacga tgccgatgcg gtagcgcatg cgccggcagt gcttctgcac
    25861 ctccatgacg accgccttgt gcacgtcctc ggcgctgccc gcggctgcgg gcgtcatcac
    25921 gatggcgacg tcctcgacgt cctcgagcgc cttcagtccg gtgctgccct tggtctcgtc
    25981 ggcttcgccg gcgtagtcga tcgcgcccgg ctcgtcgccg tcgctgccgc cctcgagctc
    26041 gatcaggtag cgcggttcgt cgagcgaggc ctcgcccggg tcgcgcgcgg cgggggcgca
    26101 gatggacagc agcgcggcga ggatcgcgcc atcggtcgcg ttcgcggcga ggtggcaggc
    26161 gaccggcgag ctcagcgcct cggcgcgacg gtcgggtgtg accgcaagcc cggcggcgag
    26221 gctggtcggc cccgtggcgg cggtcgtcag gccggggtag gtgcggatca cttcgccgtc
    26281 cttgccgccg ttgcgcacat cgacgtcgaa gctgcgcagc acgaacagcg cgcgcgcggc
    26341 accgggcgcc gcggcgagtg cggcgagggg cagcgtgacg gcttcggcga ggcccggatt
    26401 gagcgcgggt gtggccgtga agacctcgcc cgcggcatcc agtgtgccgc gcactgcctt
    26461 gcggctgccc cagcccgcac cgccggtgaa ggcggcgaga tcgacgacct cggccaggcg
    26521 caattcggcc ggcgtgcccg ccgccagtgc gccggatgcg ggcggacgcg cgaacacgcg
    26581 ggtgagcctc gcgccggcgg gcagctgggc cgggatcagg ctggtgatct ggtttgccgc
    26641 gagcgccgcg ggggcatcct cgtcggccct cgacacgatc gtggcgcgcc cgtcctggat
    26701 cgccaggttg gcgccgtcga gccggacgat ggcccgcagg tcgaccggga aggccgcgtc
    26761 gtcgatcgga ccggctgcct tcgcggcgag cgtcacgccc cgcgcctcga ggaagtagag
    26821 cgcgcctgcg cgcgcacttg ccgcggtctc gctgcgcagc aggttctcgc tgtcgcgcca
    26881 gcgcagctcg agggcgtacc gtcccatccg gcccgggaag cggctgaaga acctcagcgt
    26941 gtttccggcg tcggccttca tggcggcacc gggccacacg ccggccgcgt ccgccgcgag
    27001 gccgttgacg ccctgcatga cgcgggcgac gaagagctgc ttgccgccgt tgtcgaagaa
    27061 ggcgcgcgcc gcgtgcgcgg tgtggttgag cacggccttg ccgtccgcgc cgaaggccaa
    27121 gtcgtgcgcg tcgccgtaga tgcgttcgaa gtccgcgtag ctcgtcagca cctcggggag
    27181 accgcggagc gggccgctgc gggtggggcc caccagcgcg gcgacgctgg tgccgacccc
    27241 ctcgatggat ttgttgcgga agctcgtctc ttcgacaaag acgcctggag cgaggtactc
    27301 gggcatgtcg cttctccttc gttgccggtg aggcctgtcc acccggcggg tcagggaaac
    27361 cgcaggacga gggcttcccg tcccgggctg gccagggtgg cgatgcgccc gggcgcaatg
    27421 tccaccacga gttgtgtctt gaatgcgccg tcgccgctgc cgttgccgcg gatgcgcgcg
    27481 acggggaggg tgtcggggtt ggtgccggcg gcggcggcgc gcacctccag caggccgggg
    27541 tagctcgccg ccggggcgtc cttggtgagc gccgggagcc gctcgagcgc cagccggaac
    27601 tcgccgcgcg cgtcgctctg ggcgacaaag tcgaaggggg gggccagcgg cggggtcacg
    27661 cggagcgcga gcagggccca gggcaccgcg ctgccgtcct cgagtgcggt gcgtccgacg
    27721 agcccgccgg cgcgaccgaa gcgcgtgccg agcggcgagc ggtacagccg cagcttgtga
    27781 gcagtgcggc gaccggcatc gagcgcgaaa cgacgcggca ggtagtgccc cgcggggtcg
    27841 agcagtgctc cgctgagcgc atagagcggc tcgtccggag gctgcagtgc ccccgcgtcc
    27901 gcttcaccgt cgatcacctg ccgcagcccg gccggcacgc ggcgccacag ggccgtgccg
    27961 gtgttcttgt ggaccagctc gagatcggcc ggcgcgtcgt cgaactggat gcggaacgcg
    28021 cgcacgcaca ccggccgccg tgctccccct tgggcgccgc tcgcgtcttc ccattcgatg
    28081 aagtcgctgg catgcagcac gagggtctcg acgatcatcg cgcgctcccg tcgggcacgt
    28141 gcatcgggaa gacgagggtc ttcaccggcg gtccctcctc gcgctcgaga tcgggggcga
    28201 gacgcacggt ctttaccagg tagggggcgg acagccggta gtcgtccggc aggctgtccc
    28261 acagccgcat caggtcctcg gtgctcatct cctccgggag cacctgcaca acgtcctcgg
    28321 gcccccagtc cggatccgcg aggctcatgt cggcgctctc gagcacgagc cctgcgccga
    28381 tgatctggat cgcggcggcg aggtattcga tctcctgctc ggtgtgcgtg caccaggcca
    28441 cgagcagcac gtgcagattg accggaagtt ccgggcgggg aaggtggcgt cccggctgcg
    28501 gcggaaggtg gcggttgcgg ctgaagggat cgaccgagat gcgatgcagg tagagcccca
    28561 gcgtgtcggt gttggcctgc ggcttgccgg cgagcgtctg cgaacccagg atctcgactt
    28621 gcgggttgcc gccgaggcgc acggccatgc ggatcgccaa gcgttcccgc aatgcccgca
    28681 aggccgacag gattccgaga tgcgacgcca cgcttacctc gcttcgaggc cggagccttg
    28741 gggggacggc gttgccgggt gcagatcacc gatcaggaag ggaccggcct gttctgcgat
    28801 tcactctgaa acgtggcaca tcgcgcgaag cgcgctaaag gacgaaatgt aagtaaatgt
    28861 tttcagagcg gcctcgcccg gccgggacct gcatccgggc gggcgttcgt gcccacggcc
    28921 gggaaggtgt cgcgccgcgg ccgcgcgaag cttcaaccgg ctccgccggt gctgcgccag
    28981 gcgacctgag caagccgggc cccgagccga ggctgcaggc gcacggtgac gtcggtttcg
    29041 ccctgggcgg cttgacgatc ctgctcgagc cgcttgccgg acccggcggc ggcccggacc
    29101 aaaaggcagg gcgtcatcgc gccgtgctcg gggaagttca cgaaggctgc ccgctcagag
    29161 atcgtggagg agcggccctg ccggcacgat gcccgtcggg ttcagcgccc ggatggagta
    29221 atacccggcc ttgatgtgat cggctcgcgt gctgccggca aacgcgggca gcgccagcaa
    29281 gcggcgcagg taggccatca tgttcggatc ggcggagagc gggcgcaggt cgatgtcgtt
    29341 gtgtgccagg gcgccgaaat cgctgttcag gatctgcagg atgtcggcag actcgttctt
    29401 gacgatggtg tggcgcgacc ggtcccgcag tacgggcacg gttgcacgtc ccgtgtaggc
    29461 cgggtccgcg cggttgtaca actcgtgcat gtgatgcgca gccttgacgt cgtcgaggtc
    29521 ggtgccgagt tctccgctga aggcccagcc ctgtccggcg gatggactgt tccgccgagc
    29581 gcaacgaccg cggcgatctg cgcggcctca ccagcccggc ggcggcgcgg cgaagcgttc
    29641 ggcgaggaag tcgaccagcg cgcgcacctt gggcgggacg aactttcgcg tcgggtacag
    29701 agcatagatg ccgagcgtga cggatcgata tgcgggcatc agctccacca gcgcgcccgc
    29761 cgccagatct tcggcgacca ggaatccggg ctgcaggatc accccttggc cggcgagtgc
    29821 catcgcgcgg caggtgtcgc cgctgttggt gtgtacggcg ggtctcgtgc gcacgctcac
    29881 gcgcccgtcc ggtccgtcga actgccactc gtcgcctccg gaccagtagc tgtaggcgat
    29941 gaccgtgtgg ctggcgaggt ctgccggcag ccggggcgcg ccatgggctg ccagataagc
    30001 gggcgcggcg cacagcacga tgcgcgtgcg ggcgagctgc cggcagacga gggtggaact
    30061 gggcagcgca gcgatccgga tcgcgatatc gaagccttcc tcgacgagat cgacggtacg
    30121 gtccgccagc gtcacctcga gccgcacctg cgggtgcagg tcccggaacg cgccccacag
    30181 gggcgcgagg tgccggatgc cgaaggtgac cggggcgttg atgcgcaaca gcccgcgtgc
    30241 cgcgccactg cgcgaatgca gctcggcttc cgcttcctcg agcccggcga gcacttcccg
    30301 gctgcgggcg tagaagcgtt cgccgtcctc ggtcaatgac aggcgccggg tggtgcggtg
    30361 gagcaggcgc acaccgagtc gcgtctccag ttcgcccaca tagcgcgagg cggtcgcctt
    30421 cgacaagccg agcgcctcgg ccgcgcccac gaagctgccg gcatcgacca ccgcgcagaa
    30481 ggtctgcatc tcgacaaatc ggtccattgt ctcttcatgt ggaacagttt ctcgtctgga
    30541 tggctgttta tctcaatcgt gatggcccgt aaagtgttgc catggtcagt tcatcgcaac
    30601 aggacgccag accagcacaa ggcgacggaa cccgcggaga aacaccatga cgcctcgccc
    30661 gctcgagcgc ctgttgccca cctacgccgg ctgggccgcg cacgcgctgc gtatccgggc
    30721 ccccgggggc ccgggacgac agcggccggt cggcccgcgt catcgccgtc cacctggaga
    30781 cctctgatgc aaatgctgcc taccctgttc gtctcgcacg gcgcgcccac cttcgcgctc
    30841 gaccccggcc tcgccggcgc gaatctcgcc acgctcggcc gccggctgcc gcgtccgcac
    30901 gcggtgctgg tggtgtcgcc gcattggatg acgcgccagc cgcaggtcac gctgtcgctt
    30961 cggcccgcga ccatccacga tttcggcggc ttcgatgcga ccctctacac gctgcagtac
    31021 ccggccgaag gcgcccatct cgctgcgcgc cgtgcgctcg aactgctgca ggcggcgggt
    31081 ctgcccgcca ccccgaatgc cgatcgcggc ctcgaccacg gtgcctgggt gcccctgtcg
    31141 cacctttacc ccaaggccga catcccggtg tttcaggtat cgctgccgga atggctcgac
    31201 gccgatggcg cctacgacta cgggcgggtg ctcgcgccgt tggcaggcga aggcgtgctg
    31261 atcgtgggct cgggcagcct gacccacaac ctgtacgagt tccgcctcgg tgatccacgc
    31321 gcccaagcct atgcagcgga gttcgcgcac tgggtgcggg acgcggtgct ggcgggcgac
    31381 cacccgcgcc tgcgccaggc gctcgagatc gcaccgcacg cccgccgtgc gcatcccacc
    31441 gccgagcatt acctgccgct gctggtggcc gccggcgcgg cggcgcaggc cgtgcccgcg
    31501 agcgtcatcg aagggggcat cgtgcatggc gtgctgtcga tggattcctt cctgttcggt
    31561 accgacctcg ccgcgatgaa gggtgccgac gcgaaccagg cattgcccga ctgaggcgcg
    31621 accatgtccg atcaggatct catcgttcaa caagcgtccg ccccggcggc gcgcttgatc
    31681 ctgcttttcc atggtgtggg atcacgcccc gaggatctcg tgccgctcgg ccgcctgctc
    31741 gccgaggccg acccgggcgc ctgggtggtc agcgtgcgct cacccgaccc cggcgacctg
    31801 ggcggcggct ggcagtggtt ctcggtacgc ggggtgaccg aggacagccg cccggcgcgt
    31861 atcgccgagg ccatgccatc gttcgtgcgc caggtgcgcg actggcaggc gagggtagcg
    31921 gtggatgccg ccgccaccac gctggtcggc ttctcgcagg gggcgatcat ggcgctggag
    31981 tccacccagt tgccggaaac ggtcgccgcg cgcgtcatct cgatcgccgg ccgctttgcc
    32041 ttgccaccgc gcgtggcgcc ggcgaacacg caggtgcatc tgctgcacgg cgagcaggat
    32101 ccggtgatcg atgcgcgcta cagcctgatg gccgccgaga cgatgcaggc gctcggtgcg
    32161 gccgtcagcg tcgagttctt cccggatctg gggcatggtg tggatgctcg cgtcgccggt
    32221 cgcgtgaagg ccttggctgc ctgagtggcc cggctctgct ccatggagca tggatgcggt
    32281 agcgaacagc gtgtccggaa acgaaaaaac ccaccgcttc gggtgggttt tcatgcttgc
    32341 agcgtacctt gcgggactct tccgacgctg gccgggtccc gtcgggacat cgaatcgatg
    32401 gggcgacata ccggaatcga accggtgaca cctggagcca caatccagtg ctctaccaac
    32461 tgagctaatg ccgccactgt gggaccccgg aaaaccggac tcccgaaaaa cttttcctgg
    32521 cctgcccggc agggatcgaa cctgcaaccc ccggcttaga aggccggtgc tctatccaat
    32581 tgagctacgg gcagcttccg gcgggcagtc gggatgtgag ctggtcgggg tggagggatt
    32641 cgaacccccg acatcttgct cccaaagcaa gcgcgctacc ggactgcgct acaccccgag
    32701 agccgcgaac tatacagatg cctttttcgt tcgtcaaaca aaattcgcgc gtcgcaaaaa
    32761 aaaccggaat cacgcgctgg cgtggcttcc ggggcgcggc ccggcgtttt tcgctgcatc
    32821 gcggcgggcg cgcaccacac gcagcaggtt ggcgaagagc caaccgaact cgaccgccag
    32881 cacgacgccg gcgagcgggg cgagcaccgg cacgaagacc gcgacgcaga gcgtggcgag
    32941 actcgtggtg cgcacgcgca gctggcggcg gatcggggtg tcgggcagca gcttcttcat
    33001 gttcggtgcc ttcagcccgg cctgggtgag gtgcagccag gcgaggaagg gcacgatctt
    33061 gtagagcatc gcggtgatgg tgccgccgaa accgccgtgc aggatcagca cgccggcgag
    33121 gatcggccag cagtcggcgt cggaccacag cgagatcgcc agcaccagga cgccggcgag
    33181 cgccgagtac atggcctcgc ggaaggcgag gaagggcgtg tcgggcttgc tgcgccgcga
    33241 gtgtgcctgc aggtacagcg tggtgcccgc gtagccggcg ccgagcagcc cgagtgcgag
    33301 gatggcgatg aagcgcgcgg catcgattgc gagatactcg gcgagggtcc acaacacgag
    33361 ggtggcggtg accgccggtg cccacagcgt ggtgaagcgc tgcgggtagg cagcggtgat
    33421 ctggaacatc ggtaccacga cccagctcgc ggcggcgagc agcactgcgg cccagcccat
    33481 ccagccccag ccggcgtgca ggttcaccac ggtcggcagc ggcaccggca gggcgacgcc
    33541 gcgcgtgagc acgagcacca ggctgaagcc gaggaccgcg gtgacgacga ggccggcgaa
    33601 ggccatgcgc agatcgcgcg gggtgcgggt gttgttgccc gtgcgtgcgc cgcggctgag
    33661 gcccagcgcg gcggcggaga ggaacacgaa gagtccgccc acgagcagca cggcgccgcc
    33721 gagcaggacg ccgggtgcgc cggcacccag tccgaaggcg agccccgcgg cgccgagcgc
    33781 catcgccagg tgagtgatgc cggcgatgcg cagcggcgcg ggcatgctcg cgcccgccac
    33841 caccggcatg acctgcagca acgcgccgag catcacctgc aacatgaagc cggcggcgaa
    33901 gaggtgcgtg atggcgagtg cgccgggcgt ccaccgcgag acgaggatct cgccgccaaa
    33961 gatcagcgtc agccccgccg ccacgccgaa cagcggggcg gtgaggaaga agcggatcgg
    34021 tgccgagaac ggcggcgtgt cgtcgtagga gaggcccggc cccatcatcg ctggcggatc
    34081 cggatctcga cggcgccgtc gtcgcggaag gcctcggcgt ggcggtagcc atcgcgatcg
    34141 aggatgcgta gcagcgggtg cggcatgcgg tcgaggatga gcagcatttc ctcgtccttg
    34201 ccgagggcca tcaggccctc catggcacgc tcgaagggct cgggcggctc gaggccgcgg
    34261 gcatcgacga cacgtccgct catgctgcgc gggcctcgcc gaggtgcttg ccgaccgagg
    34321 ccgacaggcg ctcctgctcg tcgccgggca ggcggctgtc gagcatcggg tagatcacgc
    34381 tctcttcttt catgttgtgc tgctgcatca tgatcagcag cgtctcggcc tcgccggcga
    34441 agtcgtcggc gtcctgcttg tcgagggcgt actgcagggc ggccagggtg tcgcgcatgt
    34501 tctggtgttc atggcgcatc accatggtgg gaccttgcgt catgcccgtc gcctgctcga
    34561 aggcgggaaa gagggtgctc tcctcggcgt cgaagtgggc gttgagcgcg tccgcgaact
    34621 tcttcagcgc ttcggcagcg tcggcccagt cgccatcgga ggcaagctcc tcggcgcggg
    34681 cgaagatgcc gtcgcagtcg cggtggtcgt gggtcatgag ttcggaaatg gtattcatcg
    34741 gagtctcctc tctggcgggt gtatcgaatt ctccgcaggg ggcgacaaac ggcattgatg
    34801 caggtcaagg gcatcccggc tgcgttggac tgacaactgt ttgtcgtgca tcaaagtccg
    34861 catcgcttgg gcggcacggc gcttgcaggc acaatgcgcg ctttttcgca ggggacgcgg
    34921 gtggtccgga cgaggcggtc gggcggggcg gagaacggct gcaagggcgg ctcgacgagc
    34981 ggctgggtac gggtgttcgt gccgttcgcg ctcggctatt acctgtccta cctgctgcgc
    35041 acggtcaacg cggtgatctc gccgcggctc accgacgagc tcggcctgtc ggcggccagc
    35101 ctgggcctgc tgaccagcgc gtatttcctc gccttcggcg cggcgcagat tccggtcggg
    35161 atcgcgctcg accgcttcgg tccgcggcgg gtggaggccg cgctgctgtt gctcgcggcg
    35221 ctcggcagcg cgctgttcgc catggccgac tcgctcggcg cgctcgcggg cgcacgcgcc
    35281 atggtgggcc tgggggtgtc ggcttgcctg atggcggcgc tgaaggggtt cacgctgtgg
    35341 tatccgcccg agcgccagag ctcgatgatc ggtttcatca tggcctcggg ctcgctcggc
    35401 gcgctcaccg ccagcgtgcc gctcgcggcg gtgctgccgg tgctgggctg gcgcggggcg
    35461 ttctggatca tcgccgcggt gtccctgttt gccgcggtgc tggcgctgcg cgcgctgccc
    35521 gattcgccag cggcgcgtag cgaggcctcg ctggcggccg cgctgcgcgt cgtggcgggc
    35581 atcttcgccg cgcccgcctt cctgcgccat gcggcggcct cggtgttctt cgtcggcggc
    35641 ttcatggcgc tgcagagcct gtgggtggtg ccgtgggtga tgaacgtgaa cggcttcgac
    35701 atcgccggtg cggcgacggt actggtggcc ctcaacctcg gcaacctcgc cggacagctc
    35761 gcggtgggcg tgttcggcgt gcgcctggcg cgcgccggag tccagccgct cggcctgctg
    35821 cgcgcgggct acggcatgat gctggtggtg caggcgctga tcctgtggtc cgacctgccg
    35881 gtgctcccgc tgtggaccgc attcggcctg ctcaccgcgg ccaactcgca gatctacctg
    35941 tcggcggcgc gcgcctttcc gcccgcgctg ttcggccgcg tcagcaccgc gctcaacctg
    36001 atggcgttcg ccggcgcctt ctgcgtgcag tggggcttcg gcatcggcct cgatctcatg
    36061 cgaggcatgg gtgtggagct tgcgcgcgcg ctggcctggg gcttcggagc cttgctcgcg
    36121 ctgcaggcgc tcgcctatct gccgctgctc ggacgcccgg cggcgaggcc gtccaagggc
    36181 gaggcttgaa tgcccgcatg gaccgtctca ccgtgttgcg tgtgcacagc ttccttaccc
    36241 gagcgccaca aagcggcgca aaataacgcc cggccgggct ccggcgcatg ccaggcgggc
    36301 tcccgccgca tgatgtccgg gcagttccgg cgacgaattc atcctgatgg agtttcccat
    36361 gtctgaaccc acgaccgact cgaccgtgca gccgcctcgg cgcaaggggc tgcgtcggac
    36421 gttgtggacg gtcgcgatcc tgctcgcgct ctcggccgcc gcttacctcg ccgtgccgcc
    36481 ggtggcgcgg cactacgcgc agaagctgct cggcgagacg ctcgggcgcg aggtcgtcgt
    36541 cgagcgcgtg ctgatcaacc ccttccgcct tactgccgag gtgggcggac tgcgcatcat
    36601 ggaggccgat ggcagcgccg aggcgctcgg cttcgaatcg ctgcgcgcca acctcgagct
    36661 cgagtcgctg ctgcgcaagg gcatcgtgct gcacgagctc gccctcgaca agccgcgcgt
    36721 ccatgtcgcg ctcgaagccg agggtcgcca caactggacg gacgtgctcg agcgcatcgc
    36781 cgcgctcggt ggcgaggaac ccgccgagga cacgccgccg acgctgttct cgatcggcaa
    36841 catccgcatc agcgacggcc ttgtgcgcgt cgacgaccgc gtgcgcggca tcacccacga
    36901 gctctccgaa ctgggcgtcg gcgtgccctt cgtctccaac ctgccggtgc gggtggatgt
    36961 cttcgtgcaa ccctcgctct cggccaagct caacggcgac gcgctgctgc tcaatgcgcg
    37021 caccaagccc ttcgccgaat cgcaagaaac catcctcgac gtctcgctga aggagttcga
    37081 cctcacgccc tggatcgcct acctgccgat cgagccgcgc ttccgccttc cttcggcgct
    37141 gctgacgacc aatctcgagc tctcgttcag ccagacggtg gagggtgcgc cggtgctctc
    37201 cctgcgcggt ccgctgcagg ttcacaagct ggtgctgcag gatcgcgagg gcgcgccggt
    37261 cgcgaccgcg gacgaggtgg agctcgaatt cgccgacgtg cagccgatga tcgggcgctg
    37321 gcacttcacc cggctgcgcc tggcacgtcc ggagctcgac ctggtgcggc tgaaggacgg
    37381 cgggctcaac gtgctcgggc tgatatcgcc tccggccgcc gaaggcgcca ggaaggcaaa
    37441 gggcaaggct gcgaaagggg gcaaggccgc cgcgcaggtc gcgcagaagg cggcttccgg
    37501 accggccgcg ggtgcagccc aggcagacgc aggcgtcacc acgccggccg ccccggcgga
    37561 cggcaagacg gtggccgatg cccgcgacgc aaccccggcc ggagcggatt tcctgctcgc
    37621 gctcacgcgc atccgcgacg gggtggtgcg cttcgaggac cgcggcctgg ccgagccctt
    37681 ccgcgcgcgc atcgaggcga tcaacctcga catgcgcgat ctcgccaccg agggcgagct
    37741 gccggccgag atccgcctcg attacgtcac cgatgccggc gagaaattca cccacgagga
    37801 cgtgctgcgc ctggcgccct tcgagtactc gggcagcctg cagttcgagt cgatccgcct
    37861 gccgcgctat gcgccctacc tggcggcggc gatgcccggc gcctcgatcg gcgagggcag
    37921 tctcagcggc ggtgcgcaat accgcatgac gctcggcaag gacggcgcgc cggcggtgca
    37981 ggtcagcgcc gaggcgctcg cactgcgcga tttcgcgctg ctgctgaagg atgcgaagac
    38041 gccggcgctg agcctgccgg cgctcgacgt gcgcagcgcc acggtcgacc tcgccgggcg
    38101 cagcgtgcgc atcggcgggg tggcgctcca gggggcggcg gtctccgccg tgcgcaccaa
    38161 ggatggcgag ttcgacctcg ttagggcgct cgtgccgccg ccggcgaagg gggcagcttc
    38221 cgcgccggcg gccaaggaca aggccgagcc cgactgggtg atcgcggtgg acaagttcgc
    38281 gctgcgcggc gcctcggtgc ggctggacga tcgcagcgtg gcgcggccgg tgctcaccac
    38341 gctcgagggc atcgacttcg agctcgacgg cttctccacc gcgaagggcg acgccatcca
    38401 gttcaagctc gactccatgc tgaacaagcg cggcaaggtg gcggcctcgg ggccgctgac
    38461 gatcgcgccg ctcaaggccg atctcgccct cgatctacgc aacgtcgacc tgctgccgct
    38521 gcagccctac gtgctcgagc agaccaagat cgcgatctcg cgcggcaacc tcaccaccaa
    38581 gggccggctc aagctcgaca ccgaccgccg cggcaagctg ctcgcgagct tcagcggcga
    38641 cgtcggggtg gcgaacttcg cctcggtcga tcgcttgaac gcgaccgact tcctgcgctg
    38701 gcgcacgctc gcgatcggcg gtatcgacgc gcgccttgag cccctctcgc tcggcgtcaa
    38761 gcgcatcgcg ctcgacgatt tctacacccg cctgatcctc gacagccagg gccgcctgaa
    38821 cctgcgcgag atcggcggcg tcaagggtga gtccgaagcg gccgcgaagc cggcggctgc
    38881 cgtcgcgcac acgaaggagg tcgagcaggg cgagatgcag ggtggggcgg ccgccgacgg
    38941 ccagcgtact gccccgatcg cgcccccagg cccgccgccc ccgccggtgc gcatcgaccg
    39001 catcgagctc aagcgcggca acgtcgcctt cagcgaccgc ttcgtccgcc ccaactacga
    39061 cgccaacctg accgggctcg ccggcaccat caccggcttc tccacgagct cggacaagct
    39121 cgccagggtg gagatcgtcg gcaaggtgga caagagcgcg ccgctgtcga tcaccggcga
    39181 gctcaacccc ttccgccagg atgcccacct cgacatcctc gcctcggtga aggatttcga
    39241 gctcaccggg ctgtcgagct actccggcaa gtacgtcggc tacggcatcg ccaagggcaa
    39301 gctgtcggcg gagctcaact acaagatcat cgaccgccag ctcaccgcga ccaaccgcat
    39361 tttcctcgac cagctcacct tcggtgacgc ggtggacagc ccggatgcgg tgaaccttcc
    39421 ggtgcagctg gcggtgtcgc tgctcaagaa cagccgcggc gagatcgacc tcaacctgcc
    39481 ggtgagcggc acgctcgacg atccgcagtt cagcgtgttc ggcctggtga tgaagatgct
    39541 gttcaacctg atcggcaagg cgatcacctc gccctttgcg ctgctcggct cggcgctcgg
    39601 cggcggcgag gagctctcgc agctcgacct cgccgcgggc acggccaccc tgggcgaggc
    39661 ggagcagggc aggctgaaga cgctcgcgca ggcgctggtc gatcgcccca ccttgcgcct
    39721 ggacatcgtc ggccgtgccg accccgcctc cgacctcgac ggcctgcgcc aggcggcgct
    39781 gaccaacgcg gtgcgcgcgc agaagctcaa ggcgatgatc gccaagggtc aggccgcgcc
    39841 ttcgatcgag gaggtcgagg tgggcgaggc ggagtacgcc gagctgctga agaaagccta
    39901 ccgtgccgcc gacttcaaga aggagcgcaa cctggtcggc atggtcaagg acattcctgt
    39961 gccggagatg gaggcgctga tgcgcgccaa cgcgaaggtc ggtgacgacg acttgctggc
    40021 tctggcgcgc gcacgtgcgc aggtcgtgcg ggattggctg gcgggcgagg ggggcgtgcc
    40081 cggcgagcgc gtcttcgtgc tcgagccgaa ggtggaggcg ctcggcgagg gcggcgaggt
    40141 gcagttctcg ctacgctgat cggccgtgga aatcgcgact tgcgtcgctc ctacgggagg
    40201 gtgtccaggc gtgacgcctt cccgacgcgg aggttttcca cgcgcgtgcc gtttcctcca
    40261 tgcgtgcgat gtcttttcca cgctcgatgc tttctccccg cgcgacgttt tcgtaggagc
    40321 gacgcaagtc gcgacgccg
//
LOCUS       DAOQBS010000013        39689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_501_length_39689_cov_16.695640, whole genome shotgun sequence.
ACCESSION   DAOQBS010000013 DAOQBS010000000
VERSION     DAOQBS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 39689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39689
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_501_length_39689_cov_16.695640"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PKN25_02950"
     CDS             <1..872
                     /locus_tag="PKN25_02950"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNV89884.1"
                     /translation="IWRMCQVKDAPIRDWVKLAVTRARNSGMPAIFWLDPYRPHENEL
                     IKKVKTYLKDHDTSGLDIQIMSQVRAMRVTLERVARGLDTISVTGNILRDYLTDLFPI
                     MELGTSAKMLSIVPLMNGGGMYETGAGGSAPKHVQQLVQENHLRWDSLGEFLALAVSL
                     EDLGIKTGNARAKLLAKTLDEATGRLLDENKSPSPKTGQLDNRGSQYYLARFWAERLA
                     GQAEDAELAGKFAPLAKALAENEQKIVAELAEVQGKAVDIGGYYKADAEKCKAVMRPS
                     ATLNAILKGARAA"
     gene            1041..2177
                     /locus_tag="PKN25_02955"
     CDS             1041..2177
                     /locus_tag="PKN25_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89885.1"
                     /translation="MADFRKLRVMISSRCASTVRRGDSRVSMTVVRTRLKRELEAESL
                     CGERLFEVWISDNAPDQSQGDLETAWEKSLEEVRKADIVLVLYTGEAGWAPARGLGVC
                     HAEFQQAWNDGPARLKVVRIGDVGTAPKDAAELKRDQAFQAYFDDLNPTSPAASDVDA
                     IVARSREALREAVAGLVKLGGREARKGRFAYGAPLDWSRLDFGHRKKAMEDALGGGLA
                     EFGAVESSGGWLWPLGETSLLTICHGLPGGFGVAAAREMVGQPFLHDHLFLARALGER
                     EAPAGPLHLVACLKGITESQAMRQLGFPDATIVAAPFGVYVSDPVQKIQMIFLANCRD
                     LTTTRNALCRLAEWLNATGEAANLARRALGRRRVVQAILDVQGD"
     gene            2204..2770
                     /locus_tag="PKN25_02960"
     CDS             2204..2770
                     /locus_tag="PKN25_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89886.1"
                     /translation="MSSALAGLYERSGRSPPAALADWEGRVDGWCDAYLRVFPDAELS
                     EINLDLAVFQFDHVSERVTLAYALSVEPLMRRDSGRMRGFPDVNASVRRVLGDRAFVA
                     DKGHFLGHASGGILDINLFPQRRELNRGWSEEGKRFRSMERYVAEHPGTFFYHRPSYR
                     DQTWIPATLEYGVLVDGERWWVDRFRNV"
     gene            complement(2799..5162)
                     /locus_tag="PKN25_02965"
     CDS             complement(2799..5162)
                     /locus_tag="PKN25_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89887.1"
                     /translation="MNPGAPAPADDAFDAVFAIRLPPRPYPGLRPFEQHEWPIFFGRE
                     RMTDEIVDRLLGHRLLVVHGDSGCGKSSLVRAGVLPRLEQESARGGVRWRTCTTLPRA
                     APLWNLARALAALDTPGGDGHGLELRTIAFRRALNFGREAPAALAELLGCGPRSQVCI
                     LIDQFEELFEHARRHGAEEATLLAACLVGLLDAPPAGLYAVLTMRSEFLGACSRYEGF
                     AEAVNRTQYLLPRMEHDDLMRAIREPAVLYDGEVTRELAERLIVDGGGGQDQLPLIQH
                     GLMLLHDERARAAGLALAGPTPGAPAWRLGVEHYHAEHGLAGLLSAHADAVQARAEQQ
                     CLGGEATRVVEDLFRALTDINAEGQAIRRPCPLARLVAVTGAEESVLRCVVDTFRADG
                     VSFLEPYGHEALPADELIDISHEALIRCWRRIAEPREGWLAREFRNGLVWRALLVQAD
                     SFERDPGNVLGATTTDERERWLRRRNAAWAERYGGGWERVQRLLAASVEARALRAAER
                     EVAEKQRQEARRLRRRSRALAVGLSVLAFTSALGGLAWQQSRIAEAARRFAEGERELA
                     LTARDEVVRQLEALVEAREAEAAARAAAEAVAQEARQSATALASVVDELERATTAANA
                     PASGDGQAVQRSLTEAKSELRAQVSNLSNLSAQQAAPPVSAAPAPASGGVAPRLWIYI
                     AEEGQRPTAEALEARLRSVRIGDAALELPGIVLVKAAPARSMLRCFRTEECRQDGEAL
                     ARALAALLQSPTPSLEDFSALYGSSGSIRARHYELWFAPGAIVLSAR"
     gene            complement(5159..6259)
                     /locus_tag="PKN25_02970"
     CDS             complement(5159..6259)
                     /locus_tag="PKN25_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNV89888.1"
                     /translation="MSYVGEPFRHDLFVSYSHGDDGSGGACLAPWSAAFAHELERELR
                     ADRRFRTSLSVFRDQDHRPAQSLDPMAPLTAQLQEQIAGAALLVVLMSPDYLASKWCA
                     DERDWWLQRQAALGLPHEERIAVVRIWPTDTPWPAALCDARGEPLVGFAFYPADGVPP
                     RPLGWTDLPGAFGSEFRKALLDMVGRLYPKLEAMRARTEELRRQREEAERLVRDGGQL
                     LYLHGRSDHAAAWEEAALKLVDKGFAVVPGEPDRVDSDPLRLQAVRERRVETLSGCDA
                     LLLLGTEDGRALDADLVVVGKHDRQSARARSHRLLPCGLLDTAGATIATNVRRATARI
                     VQADWIDGTDAGWPVAVRDWLGGKGLQAGGAP"
     gene            complement(6398..7525)
                     /locus_tag="PKN25_02975"
     CDS             complement(6398..7525)
                     /locus_tag="PKN25_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HNV89889.1"
                     /translation="MSKRLHPLSSLLRAAASLAAAAVAAIALAAPPGALPALNIAIED
                     TSVSGISSGGFMSVQMQVAHSGIVRGAGVVAGGPYYCAQDSVLKATTECSCTGAPTLS
                     CKVGEASTAVPELVAATRAFRAEGRIDDPAQIARQRVVTVVGGKDALVPAAITRQLHG
                     YYDAFGLPAAALKPVTLDAAGHTMPTTAYGGACGTTDEPYIGSCGFEAAHAILEWIYG
                     PDVTPPRTTPTPAGRFVEFDQRAYIPATGFFSHLWGTGLDKTGWVYIPPACAAGERCR
                     LHVVFHGCKQGQSFLPLRRPPDGGLYNGTTFVRNTGYDRWADTNRLVVLFPQAVSIPW
                     KNPNGCWDWWGYTDAHYATRDGIQIRSVRAMIERLASGRHD"
     gene            7735..8652
                     /gene="menA"
                     /locus_tag="PKN25_02980"
     CDS             7735..8652
                     /gene="menA"
                     /locus_tag="PKN25_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HNV89890.1"
                     /translation="MTPPHPAPSRPGTLAVWWAAIRPRTLGMALAPVMLGTALAVADG
                     AELAWAVLFATVSCALLIQIGTNLYNDAIDFERGTDRPERLGPLRVTLAGWASAAEVK
                     RGALLCLLLALLLGAWLCVVGGALILAIGLASLIAGWAYSGGPRPISHSPLGELFVLL
                     FFGVFAVLGSHWLQSRALGHLDLLAGLAVGAPAAAVLLVNNYRDLAGDLRAGRRTLAA
                     LLGAEGARRAFALLVLLPIPTLILIAASGAPGAILGLVGAPALAPLVWRFGRAEGGPA
                     LNALLVDTARAGTRTGLLAAAGIVFVSSL"
     gene            8662..9609
                     /locus_tag="PKN25_02985"
     CDS             8662..9609
                     /locus_tag="PKN25_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNV89891.1"
                     /translation="MSIDVLLPAALAFIMFSVGLALQGADFHRVFARPRALLIGLLGQ
                     LVLVPLAALAVVLAFDLPVLLGMGLMVLAACPGGASSGFLTHLARANAALSLSLTVIS
                     SLAALLTFPLLVKAVLAAFGEGVFGADGSVLAELPVGRLIGSVLVVTTVPIVAGMLLR
                     RRAPALTARAEPLVARVATLFFAAIVVATFVSHRHTILANLLSVGPATLVLNFVVMGL
                     GYGLVMLAGAERRDAVAVAMECGLQNAGLAIFVAIVLLRQPELAVGAVVYALTMNFGA
                     LGLVFVARRREGALAHDAKRVLRLPPGPPTGSRATPPRR"
     gene            9751..11130
                     /locus_tag="PKN25_02990"
     CDS             9751..11130
                     /locus_tag="PKN25_02990"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNV89892.1"
                     /translation="MSTSDLPRKPTGVLAATLLILFGLALFVALAWSSHASLRELEAV
                     TDARSRSRNGLLLAAEAMSALKDVETGQRGYVLTGDEHYLGPFETGTRSLDETFEALR
                     RTLDDLPQARLETLWTLVEHRVALAERNIADRRAYAEENSPWKQRLDEGRDTMDRIRA
                     RFAGIEAMLRAEIEARDRSLSALRAHALLLNVLLPAIGGALILAAWGLLQRERRRRVD
                     AEAALLAANARLEDTVAQRTVELQRALGEIEAFAHGLDRSIEDERRRLAREVHDQLGQ
                     LLTALKMMVHRALQGAPGREQDWPQIEAVLGEGIATVRRISAALRPPLLDDLGLEAAL
                     GLAGRQFAERTGLACEVALVDAGQLSADQANQLYRIAQEALTNVARHAQARRVRVEGV
                     AVDGEYRLAVEDDGVGMAPQARSSLGLLSMRERAALAGGELHVGSGAGGGVRIEARVP
                     LIATGGEGG"
     gene            11127..11768
                     /locus_tag="PKN25_02995"
     CDS             11127..11768
                     /locus_tag="PKN25_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV89893.1"
                     /translation="MKLLIVDDHPVMRFGVRQLVTQRWPDAVIGEASCLAQALEQVRA
                     AEWELAVLDLSMPDTSGLEGLVRLHRAAPLTRLLVLSMHDEAAYAARALQLGAAGYLT
                     KEHATTELVAAIERVRGGQRYISSDLAARLADLLSGEAPARAPHETLSAQEYRVLLLI
                     AAGHGASEIAEIMHLSVKTVGTYRSRIIDKTGLANTAEMARYCSEHGLVDRGG"
     gene            12201..14414
                     /locus_tag="PKN25_03000"
     CDS             12201..14414
                     /locus_tag="PKN25_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV89894.1"
                     /translation="MRIVIVEDSPLLRDNLIRLFADHPELSVLGSAAGEDAAVALIAA
                     QRPDTVLLDLSLSQGSGLGVLRRLRATPGLAPRVLVLSSRPADPWEALCLDAGADAFL
                     DKAGDPSVLLAQLASWMPPLPLDELRRRRRLQQLQVLDTRAEPALDAIARLAARIAEA
                     PVGVISLVDTHRQWFKARVGVEVQTTSRSVSFCAHALLGDGLFEVEDATLDPRFADNP
                     LVRGDPGIRFYAGMPLVMPGGEVVGTLCVIDSRPRRLGATAREALAVLARSVTDELEL
                     RQRVGELEEEVARRHEAEARIMQLATRDALTGLPNRAALMDRLHQGVRAAQRSGNLLA
                     VLFLDLDRFKWINDTLGHDLGDALLQEMALRLSRAVRECDTVARLGGDEFAIVLGDLW
                     QVADAEAVAAKIVDVITRPLEVRGHALRVGGSVGVAVYPFQAHDEESLLRHADLAMYH
                     AKESGGNRHQLFSEQMNARAVERMTVESELRAAIAAGQLELHYQPQLCLEDGRLAGME
                     ALVRWRHPRLGLLAPGRFVPLAEDCGLIWELGLAVLELALAQLAAWDRAGLEVPRIAV
                     NVSPAQLRPELVERIVEILDRHGVAARRLEIELTESALTADGPAANGLLQALRRMGAS
                     IAIDDFGVGYSSLTLLRRLPISALKIDRSFVAELATNRQDAAIVEAILRMAHSVGLRT
                     VAEGVEHAEQQAALGELGCQEVQGYLCARPLEAKAASAWLAGQHAGGLVPSAAAA"
     gene            complement(14457..14996)
                     /locus_tag="PKN25_03005"
     CDS             complement(14457..14996)
                     /locus_tag="PKN25_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNV89895.1"
                     /translation="MAQEIERRFLVRDVRILDGRRGEPIIQGYLAKEVGSMSTRVRIR
                     GERAFLTLKSPKQGFSRDEFEYPIPVDDAHQMIVRHCAGRVVRKTRYLVDHAHHVFEV
                     DVFQGHHAGLVVAEVELPHEETPLCLPHWIGDEITHDSQYGNFTLAEQAALLHGTPHR
                     THAAPTPVVVAPRSLPAAH"
     gene            15236..15901
                     /gene="parA"
                     /locus_tag="PKN25_03010"
     CDS             15236..15901
                     /gene="parA"
                     /locus_tag="PKN25_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HNV89896.1"
                     /translation="MSAPTLIALISQKGGSGKTTVAMQLAAGLALEGYRVALADLDPQ
                     ESASRWAESAPADAPFPARVVRLSGSADEMGKTLRPVANKVDVVVMDCPPSIEHAHTM
                     SALALCDIALVPVVPGPTDLWATRGIERLILERKRSRPGLRGALLPNRVTRTALSADV
                     LEVLRDFTLPVLDAALSQRSAYALGAVRGTSVFGLGRSAAPAQEEVARLVAAVVKLIE
                     ERG"
     gene            15898..16395
                     /locus_tag="PKN25_03015"
     CDS             15898..16395
                     /locus_tag="PKN25_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89897.1"
                     /translation="MSATSSGLKSKLRETLKQEDAALAERSRAVAKPAAAQPRVGKPD
                     AAEPAATATPTTAAQALQPTVGGSAAAEKRATATLLELAAPAAAGRRDKGEKVERDSF
                     SMPAREHKRIKALREALGEHGVLVSKSALLRAGLALLAERDTGEIARLVAALPKVAKG
                     KRKRR"
     gene            complement(16554..18074)
                     /locus_tag="PKN25_03020"
     CDS             complement(16554..18074)
                     /locus_tag="PKN25_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNV89898.1"
                     /translation="MDPDLGTELFESLGLDALLPESLSAWRPLLLEGMAFFLDRLPEA
                     RLGAIVAEQLALAASVSAAARATALLARCPTLHKLGQVVARQPGLDAELRRHLQALES
                     LPASTDDGALHARIRAELGAGHGLHLADRALAEGSVAVVLPFSWRQDGRSHEGVFKAL
                     KPGIGERLAEELAILPALGDFLERRGAEFGLPAIDYRSHLAAASRLLVQEIRLDREQA
                     NLRAASALLAADTDVFVPALLPWCTPALTAMERIDGPKLADAALSAHESRRLGRALVG
                     GLLARPFWSCGEDAPFHGDLHGGNLLLAPDGRVAVLDWSLTARLSKAEREALVEVALA
                     ALALDASRIMAGLAGLGLRDTRAAPIGATVEHALDTLVRSGRPLGFDWLVGLLDALAL
                     QGAGGFGEQLAVFRKSWLSLAGVLGDLGAGHDTDLPLLGLGLQRFAAEWPARLLAEPD
                     SRAFSTHVSTADLLRAAADTWPAGLRYWLRLLGFAGAENRDARRADGKPTRLPQGL"
     gene            complement(18104..18535)
                     /locus_tag="PKN25_03025"
     CDS             complement(18104..18535)
                     /locus_tag="PKN25_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89899.1"
                     /translation="MSTHTRSALRASLKQEDVALDERLPEIAGTRPPTRPETKAPPLP
                     AEPAKAKPEKRKRESFALVASDLRRLDALRSTMKAAGRTVRKSELMRAGLAALAALDH
                     AETIALLDALPPLKRTAAKDDEKARAGKKKSSQRKAAARKR"
     gene            complement(18620..18829)
                     /locus_tag="PKN25_03030"
     CDS             complement(18620..18829)
                     /locus_tag="PKN25_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89900.1"
                     /translation="MQTHAHLTAPGTPTGDRLGLHAGRESLGAQLTARWQSRTRKHVA
                     DLLDDADALADWHRNLERLRRGEHG"
     gene            complement(18907..20646)
                     /locus_tag="PKN25_03035"
     CDS             complement(18907..20646)
                     /locus_tag="PKN25_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV89901.1"
                     /translation="MVSNGRNGLPDGRAVYRHIAAIGEGLTARADPFGIAAPILHAQM
                     AWLMHPQELGERLAGLGTDLWQLQWHTWRRALGLPSADPVKPHADDPRFADPVWTESA
                     TWDLVKEWYLAFTHHVQDMLYETPGLSGKERRRAAFWWRKWLNAVAPTNYLWSNPIAL
                     RRFIDTRGHSLWQGWQNMLADLQAGNVRMTDPDGFHVGDNLGTTPGAVIFRNRLLEVI
                     HYAPTQPQVHAEPVVIVTPWINKFYILDLNPRKSMVRYLLDQGLDVYITSWKNPDASM
                     REVRFDDYLVEGISAIVRVARECSGAERVHAVGYCIGGTALATWMAWAARHYGEDAVP
                     VRDWTLFTTLVDFHKPGDIEVFIDEESVAHLSEQMKEKGYLDGKDMAASFRLLRSNSL
                     IWHYVVHGWLYGESPAPFDVLYWNMDTTRMPAAMHAWYLRELYLNNRLVQPDALVVAG
                     EPIDLRRIRQPLYVVAAEDDHIAPWAQTFHTVHHVAADKRFVLSSSGHILGIVNPPVT
                     PPRRHYRVATAHRADRAPLWRERAALREGSWWEDWMAWLKPRAGELVAARPAADERHP
                     ALGDAPGTYVLEA"
     gene            20976..22904
                     /locus_tag="PKN25_03040"
     CDS             20976..22904
                     /locus_tag="PKN25_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNV89902.1"
                     /translation="MEKIEAVASLGQAELLRPARVRAALAANDRLKLYLSVLQAAHDC
                     AQRGDARALDLAREFAVAGVDAPWLAQLPATAYREGKELHVAGLDRLAGLLAEDLCTM
                     ARPLEGDADFAARCAHWCAWLEALAPDTLSSAQLHALTSGKRGKDDSLHLLVMDLHKA
                     LNRLASELSSETIDGAHVWQVDAADRSRIAAFMRGLNATRALKLDHPGLDTAATRDGA
                     RLLLQNDIGTNDAHVLVIQVEGLRVVLTYSDLHRRRFAFFQSLLCEVGANWSEPQART
                     SAGLNFGATYYVGTATFDCADEEALCGVLEGIGTRIVFLIDWNRARKRLEQFVGKTDA
                     VAILAEAARRRVGHMAWLAAGGEQLVFGAMQALGPEYFRIGDRLDGVLGTEKARAFVL
                     ESMALASAAMQQRQPLALVADDTRLLLARYLHRHDEFDLLAEHAAYCHALAEGLRDGL
                     AHGHERDRKAAREFSERAKDWERRADQLVMDARARAEARPRWEAFAELVETADDVADA
                     LEEAAFLLSLIAADHHQGWRGEVHQTMQLLADAVLGAAQDHVRALEIARGFGEDSSAE
                     DHEAFVAALWRVLNAERQCDVLLRDVRRALAEHVSDAATLNLSTDFAQALESATDWLL
                     ATGYGLRRLVFSRAGVGR"
     gene            22931..24586
                     /locus_tag="PKN25_03045"
     CDS             22931..24586
                     /locus_tag="PKN25_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV89903.1"
                     /translation="MMAHPDAASAAAPADARRVVRLIHADAPLPADCSAEALGSKAWN
                     LARMAALGLPVPPAFVIGTGWCAEPGALAEAAWRPALAELERLSGLRLGDTRHPLLLS
                     VRSGAPVSMPGMMETLLDIGLTETTLSGLLRVSGHPRLTWDAYRRLIAGYGEVVAGID
                     AQHFDADLEAVRGECGECGQGDERVLDFAALRELAARHLDTYRREAGEAFPQDAHEQL
                     RQAIAAVFASWHADKARAYRAMHGLADDMGTAVTVQRMVFGNAGGLSGAGVGFTRDPS
                     SGEPRPWVDFLFNAQGEDVVSGRRSAHGHDELAAVAPAVWQAVLDVTARLERAFGDMQ
                     DFEFTVQQGVLHLLQTRAGKRTPQAAARIALDLLDEGVIDAETARVRTAALDVRALAE
                     HRIVRADGAALVPLGRAASAASGVACGEIVFDEARARARHAAGAKLILVRQDAETRDL
                     AALELAEGLLTCRGARTAHAAVVARQLGKVCLVGCEALRLDEAGRTLELGGHILREGE
                     LLTLDGNEGAIHAGAAAVVEVVPQALLARLDALHGREVAALQS"
     gene            24613..24861
                     /locus_tag="PKN25_03050"
     CDS             24613..24861
                     /locus_tag="PKN25_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89904.1"
                     /translation="MSFRSSYGHKCAIELYRDATERQAKLAGVTIPTFMCRRCGKRQS
                     VSGRRQVVRGSSRYGYHCASCSTARPAGAGCESRPSQV"
     gene            complement(24884..26596)
                     /gene="leuA"
                     /locus_tag="PKN25_03055"
     CDS             complement(24884..26596)
                     /gene="leuA"
                     /locus_tag="PKN25_03055"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNV89905.1"
                     /translation="MMLQNPAAKYRPFAPIALADRQWPNKLIERAPIWMSTDLRDGNQ
                     ALFEPMNVERKMRMFRTVCEIGFKEIEVGFPSASQTDFDFVRALIEGGHIPADVTIQV
                     LTQAREDLIRRTMESVRGAPRAIIHVYNATSPTFRELVFGMEKPQVLALAVSAVRLIK
                     QIGAEMEKECGTEIRLEYSPETFSATELDFAREVCDAVTAEWGATPENKVILNLPATV
                     EVATPNVYADQVEWMHRNLARRDSVVLSLHPHNDRGSGVAAAELGLMAGADRVEGCLF
                     GNGERTGNVDIVTLALNMYTQGVSPGLDFSDINAVARTVEYCNQLPIHPRHPYVGDLV
                     FTAFSGSHQDAIKKGFAAQKAGQPWNVPYLPIDPADVGRSYDSVIRVNSQSGKGGIAY
                     LLETEYGIVMPRRLQVEFSREVQQVTDASGGEMSAAAIWDLFRSTYLDNVEPVRYVEH
                     HLFEHGAAQGIRLVVEFGGTRHLLVGEGNGPIDAAIHALRGAGIDVQVRSFEERSVAP
                     SEDGGNAQACAFLELTEAGGQGGDRYGVGIDANIVTASIRAILSGVNRLQPDCARAPA
                     ARAA"
     gene            26752..27237
                     /locus_tag="PKN25_03060"
     CDS             26752..27237
                     /locus_tag="PKN25_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNV89906.1"
                     /translation="MERPIVQLDRYDRQILELLQQDGRISNQELADRIALSPSACLRR
                     LRALEESGLIRGYRALLDARKLGLSLMALIHISMDRHTPERFSHFEAEVEKIPQVLEC
                     LLITGQDADYQLKVVVTDMDGYQALLLERITRIPGVTGVHSSFVLRRVIDKTALPLPG
                     A"
     gene            27393..28592
                     /gene="hmpA"
                     /locus_tag="PKN25_03065"
     CDS             27393..28592
                     /gene="hmpA"
                     /locus_tag="PKN25_03065"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HNV89907.1"
                     /translation="MDARVRELVKATIPVLKEHGVALTTYFYQRMFRHNPELKNIFNQ
                     SHNETGKQPVALAMAVLAYAENIDDPSVLAPVITLIANKHASVGIRAEHYPIVGKHLL
                     ASIREVLGDAASDELIAAWGVAYGALADVFIDAESKLYNQACNADGGWSGWRSFRIID
                     KVRESEEITSFHLVPCDGGPLPAYRAGQYTTARVFVPELGVMQLRQYTLSIAPGAMHF
                     RISVKREPAGATTPAGRVSNRLHDFYKVGDMLELAPPFGEFFLDETHDGPVVLISGGV
                     GITPMISMLDRLVAAAPSRPVQFVHACRNAAVFAFGAHLAKVAERNPQVRLHLFHDEG
                     AVSAPVQAGRVDLRALAGEVLAEGADYYLCGPVGFLEAQIATLHELGVESARIHAEVF
                     DTGGIAT"
     gene            complement(28791..29627)
                     /locus_tag="PKN25_03070"
     CDS             complement(28791..29627)
                     /locus_tag="PKN25_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNV89908.1"
                     /translation="MTRNTRIARIRRSLAGIATVAIGLFAAPAIAQQAASIVVASTTS
                     TEQSGLFGHILPQFKQATGIDVKVVALGTGQALDVGRRGDADVVFVHDADAERKFVDE
                     GHGIEHRKVMYNDFVLIGPKDDPAAAKGKDITGALQKLAAANGNFVSRGDKSGTHAAE
                     LRYWKAAGVDNKGSGYKECGCGMGPALNMAASSGAYVLADRGTWLSFKNRADLAVLVE
                     GDTRLFNQYGVMVVNPERHPHVKQAEARKFVEWVVSPAGQEAIAAYKIEGEQLFFPNA
                     GQ"
     gene            29821..30780
                     /gene="paaX"
                     /locus_tag="PKN25_03075"
     CDS             29821..30780
                     /gene="paaX"
                     /locus_tag="PKN25_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HNV89909.1"
                     /translation="MKSRVIERWVEAHLAGRRISANSLIISVYGDLIAVHGGVVWLAD
                     LIRLMAPFGLNERVVRTSVYRLVQDDWLESGQVGRRSYYRLAASGMRRFAPAWRRIFE
                     PEGEGWSGTWQVVMLPSALEARVADNLRRELSWAGYGALNAGVLLRPTDDASTLRNIL
                     DGCGLRDRVVPLTARGLDGVTGAATDELVRECWDLQAIAATYDGFIEHFRPLLRAFGA
                     GEAEPEQAFLVQTLLMHEFRRVLLHDPQLPVQVMPNRWRGDAARELCARLYRLSWRAA
                     CRHLQASCATPEGPLPPPSAQFFRRFGGLELPPAATDEVAAEG"
     gene            complement(30815..31720)
                     /locus_tag="PKN25_03080"
     CDS             complement(30815..31720)
                     /locus_tag="PKN25_03080"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HNV89910.1"
                     /translation="MKPNRSPLPGQPAPRLQQHWDWRAAGNFICGGSGGGLVLFAGLA
                     GLAGVEVRPQLAAGLLLVALGLLCVWFEIGRPWRALNVFRHIRSSWMTREASVALALF
                     GAGVLALYTAHPAAQALAGLCGLAFVYAQARILAADKGIPAWRHARCAPLVLGTGLAE
                     GAGLIATTLAGGLQIAAAMALVLALCARLLLWRRYLDGLRADRAPAPSVQAFEAIAAR
                     MVRVGHLLPAGLALLAAAGILAGVPGAGSALAAAGLLAAGAGWWFKYTLIRRAAFTQG
                     IALKHHPVRGGKPAGLASAPRLGNA"
     gene            complement(31720..32367)
                     /locus_tag="PKN25_03085"
     CDS             complement(31720..32367)
                     /locus_tag="PKN25_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV89911.1"
                     /translation="MTRYVMSIDLRRCVGCQTCTAACKNANATPPGVQWRRVLDLETG
                     EFPDVRRSFLPTACMHCAEPPCAAVCPTEATQKRRDGLVTIDYDVCIGCANCVMACPY
                     EARSIVHEARFAYGEQPIASEAIRFDPSRISVATKCSFCKERIDAAAASGRIPGRDPE
                     VTPACVNSCISGAMVFGDIDDPDSAVSRLLAKTQHFHMHEELGTGPSVYYIWDHA"
     gene            complement(32364..35117)
                     /locus_tag="PKN25_03090"
     CDS             complement(32364..35117)
                     /locus_tag="PKN25_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV89912.1"
                     /translation="MTAATARNERKIPSYCYNCVAGPDFMTVKVIDGVATEIEPNFAA
                     ADVHPARGRVCVKAHGLVQKTYNPHRILQPMKRTNPKKGRNEDPGFVPISWDEALDTI
                     AARLAAVREKGLVDDSGLPRVAASFGHGGTPAMYMGTLPAFLAAWGPIDFSFGSGQGV
                     KCVHSEHLYGEFWHRAFTVAADTPNCRYVISIGSNVDASGGPCAVTRHADARVRGYKR
                     VQVEPHLSITGACASEWVPIRPKTDPAFMFALIHVLVCEHSLGQLDLPFLRDRTSSPY
                     LVGPDGLYLRDPDSAKPLVWDPAAGRAVPFDTPGVEPALEGRFRVAAAVTVDADDARH
                     ALVDVEGTPAHTMLVEHMRKYTPEWAEGICDVPATTIRRIAGEYLENAQVGATIEVDG
                     ATLPLRPVAVTLGKSVNNGWGAFECCWARTVLATLVGALEVPGGTLGTTVRLNRPHDD
                     RHLSVAAGEDGFMAQKFNPTDKEHWVARPTGRNAHRTLVPIVGNSAWSQALGPTQLAW
                     MFQREVPRDFNMPKPTLPDIWFIYRSNPAISFWDTPSLVETIATFPFTVSFAYTVDET
                     NFFADLLLPEATDLESLQMIKVGGTKFVEQFWTARGVVLRQPAVEPQGEARDFTWIST
                     ELARRTGLLEPYNKAINRGAGGVSPLAGEGYDFSLDPTRTHDVDTIWDAICRAASTDL
                     SQGQETHDLAWFKEHGFYTVPMPQRSWYLTPTLAEKGLRYELPYQERLLRIGRELGNR
                     LHEHDMHWWDTQLSEYTALPEWHDVPGRWEQALVDSGAKPEDYPLWLLATKSMQYHTG
                     GNVSIALMREVAQNVRGHAGVIMNANTARRLGIADGDRVEIRSHIGATYGKAVLAHGI
                     RPDTLVIPGQFDHWATPVAKDFGMPSLNTVAPMSLELTDATGSGADIVRVAIRRLEGS
                     TVQ"
     gene            complement(35132..35950)
                     /gene="fdhD"
                     /locus_tag="PKN25_03095"
     CDS             complement(35132..35950)
                     /gene="fdhD"
                     /locus_tag="PKN25_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNV89913.1"
                     /translation="MLDHPSPASGAHRSYGVERRGTDSAILGEDAVAEEAPVALVYNG
                     FSHAVMLATPQDLEDFALGFTLSEGIAASARELHDIDVVEHALGCEVRMALAGERFAA
                     MRERRRALAGRTGCGLCGIESLAQLQRPLPPARLADTAIQPGALERAQAELAALQPLF
                     QLTGAVHAAAWCRRDGSVALVREDVGRHNALDKLIGAIATRGCGFDDGFVLMTSRASY
                     EIVQKAATVGIAAIAALSAPTGMAVRLAEAAGVTLVGFARGHRHSVYTHPQRMH"
     gene            complement(36199..36807)
                     /locus_tag="PKN25_03100"
     CDS             complement(36199..36807)
                     /locus_tag="PKN25_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNV89914.1"
                     /translation="MPARTRGLIVGILLAAGAGSRFGGGKLVARLADGRGVAATTCAR
                     LLPAVDLVVAVVPAQPGTLEDELRAAGAEVVRCGAEHPGMGVSIACGVAATADAAGWL
                     VALGDMPLVPTAQHRLVADALRSGAAIAAPVCRGRRGHPVGFAARFGDELRALAGDEG
                     ARALLARHRGALVELAVDNDASWLDIDTPSDLAAVNHRLGSH"
     gene            complement(36783..37784)
                     /locus_tag="PKN25_03105"
     CDS             complement(36783..37784)
                     /locus_tag="PKN25_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNV89915.1"
                     /translation="MDSVNLETLRQAVAWQAAGLPVTLATVVRTWGSSPRPEGALLAI
                     CGDGRLAGSVSGGCIEDDLSDRLRTQWLERPELVTYGITADQTRRFQLPCGGSLSLVL
                     EPLHAGSRLAEALARIERGELLARRLSLATGAVELLDSVPDTAVVFDGSRLTAAFGPR
                     WRMLIVGAGQLSAYLAQFALALDYRVTLSEPREEYRASWTLPGVEITTEMPDDAVAAM
                     RPDRRSVILAAAHDPKLDDLALIDALHSDAFYIGAVGSRATSAARRERLRLFDLDDAQ
                     IARLHAPAGLPLGSRTPPEIALAIVADLTARRNGTSLTAAVARPATESAACPLARAA"
     gene            38025..39149
                     /locus_tag="PKN25_03110"
     CDS             38025..39149
                     /locus_tag="PKN25_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNV89916.1"
                     /translation="MRRIRLNYVLGADTGSAPLHNPLLDLLQAVREQGSISAAARVLD
                     LSYRHVWGELKRWELELGQPLILWEKGQAARLSEFGAKLLLAERQVQARLLPQIEALR
                     ADLERAFAIAFDDSVHVLSFHASHDEALAALGEEARTRGLHLDIRFTGSVDAIRALNE
                     GRCTMAGFHVRLPAVPGSRGSSSHSQRTYKPLLRPGLHKLIGFARRSQGLIVARGNPR
                     GLHGLADLARPGVRFVNRARGTGTRVIFDELLGELGLAPAAIEGYANDEPSHAAVAQA
                     VASGQADAGFGIEASARSRGLDFVPLVEEAYFLACLKSTLEHDATRALLALLRTAAWQ
                     QRLADLPGYAPMQSGEVLSMSRVLPWWRFGGRGNAREGKQ"
     gene            39263..>39689
                     /locus_tag="PKN25_03115"
     CDS             39263..>39689
                     /locus_tag="PKN25_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV89917.1"
                     /translation="MLYPTRFDVIVVGGGHAGTEAALAAARMGAATLLLTHNIETLGA
                     MSCNPSIGGIGKGHLVKEVDALGGAMAAATDEGGIQFRILNASKGPAVRATRAQADRV
                     LYKAAIRRRLENQPNLWLFQQAVDDLTVVGDRVTGVVTQI"
BASE COUNT         5399 a        13599 c        14827 g         5864 t
ORIGIN      
        1 acatctggcg catgtgccag gtcaaggacg cgccgatccg cgactgggtc aagctcgccg
       61 tcacccgcgc gcgcaactcc ggcatgccgg cgatcttctg gctcgacccc tatcgtccgc
      121 acgagaacga gctgatcaag aaggtcaaga cctacctcaa ggatcacgac accagcggcc
      181 tcgacatcca gatcatgtcg caggtgcgcg cgatgcgcgt gaccctcgag cgtgtcgcgc
      241 gcggcctgga caccatctcg gtgaccggca acatcctgcg cgactacctc accgacctgt
      301 tcccgatcat ggagctgggc accagcgcca agatgctgtc gatcgtgccg ctgatgaacg
      361 gcggcggcat gtacgagacc ggtgccggcg gctcggcgcc caagcacgtg cagcagctcg
      421 tgcaggagaa ccacctgcgc tgggattcgc tcggcgagtt tctcgccctg gcggtgtcgc
      481 tggaggacct cggcatcaag accggcaacg cccgcgccaa gctgctcgcc aagaccctgg
      541 acgaggccac cggccgcctg ctcgacgaga acaagtcgcc ctcgcccaag accggccagc
      601 tcgacaaccg cggcagccag tactacctcg cccgcttctg ggccgagcgc ctcgccggac
      661 aggcggaaga cgccgagctg gcgggcaagt tcgccccgct cgccaaggcc ttggccgaga
      721 acgagcagaa gatcgtcgcc gagctcgccg aggtgcaggg caaggcggtg gacatcggcg
      781 gctactacaa ggccgacgcc gagaagtgca aggcggtgat gcgtccgagc gccacgctca
      841 acgccatcct gaagggcgcg cgcgccgcct gaggcggttg atgtgccgcc ggccagcgcc
      901 cggcggccgg cgaacccgaa aaggggcaga tcgacgaatg cgtcgacctg cccctttttc
      961 gcgtccccac gcaaggcaag ggtatgcacg atcgccgccg ggtgcctaac atgacagcct
     1021 cgccttacga ggacaggccg atggccgatt tccgcaagct ccgcgtgatg atctccagcc
     1081 gctgcgcgtc cacagtgcgc cgtggggatt cacgggtttc gatgaccgtc gtccgcacgc
     1141 ggctgaagcg cgaactcgaa gcggaaagcc tctgcggcga gcggcttttc gaggtgtgga
     1201 tctccgacaa tgcgccggat caatcgcagg gcgatctgga gaccgcgtgg gagaaatcgc
     1261 tcgaggaggt gcgcaaggcc gacattgtcc tggtgcttta caccggtgag gcgggctggg
     1321 cgcccgctcg cggcctcggc gtgtgccatg cggagtttca gcaggcttgg aacgatgggc
     1381 cggcgcggct caaggtggtg cgcatcggcg acgtgggcac ggcgcccaag gacgccgccg
     1441 agttgaagcg cgatcaggcc ttccaggcct atttcgacga cctcaatccc acctcgccgg
     1501 ccgcttccga cgtcgacgcc atcgtcgcac gcagccgcga ggccttgcgc gaggcggtgg
     1561 ccgggctggt caagctgggt ggacgcgagg cacgcaaggg gcgtttcgcc tacggtgccc
     1621 cgctcgactg gagccggctc gatttcggcc accgcaagaa ggcgatggaa gacgcgctcg
     1681 gtggggggct cgccgagttc ggcgcggtcg aatccagcgg gggctggttg tggcctctgg
     1741 gcgaaacctc gctgctcacc atctgtcatg gtctgccagg gggcttcggc gtggcggccg
     1801 cccgggaaat ggtgggccag ccctttctcc acgaccatct gttcctcgcc agggcactcg
     1861 gcgaacggga agcaccggcc ggtccgctgc atctggtcgc ctgcctgaaa ggcattaccg
     1921 agagccaggc gatgcgccag ctgggcttcc cggatgcgac catcgtcgcg gcacccttcg
     1981 gtgtctatgt gtccgaccca gtgcagaaga tccagatgat cttcctcgcc aactgccgcg
     2041 atctgaccac gacccgcaat gccctctgtc gcctggccga gtggctgaac gccaccggcg
     2101 aggcggcgaa cctggcgcgc cgtgcgcttg gtcggcgcag ggtcgtgcag gccatcctcg
     2161 atgtgcaggg cgactgagat cgcccgaacg ataggaaagc atgatgtcgt ccgcgcttgc
     2221 cggcctgtat gaacgcagcg gtcgcagccc gccggccgcg ctggcggact gggagggccg
     2281 cgtcgatggc tggtgcgatg cctatctccg ggtcttcccc gatgccgagc tgtcggagat
     2341 caacctcgat ctcgccgtgt tccagttcga tcatgtgagc gaacgggtca cgctcgccta
     2401 tgcgctgtcg gtcgagccgc tcatgcgtcg cgacagcggg cgcatgcgcg gctttcccga
     2461 cgtcaatgcc tcggtgcgcc gcgtcctcgg ggatcgagcc ttcgtcgcgg acaaggggca
     2521 tttcctcggt cacgccagcg gaggcatcct ggacatcaac ctgttcccgc agcggcgcga
     2581 gctgaaccgc ggctggtccg aggaaggcaa gcgcttccgc agcatggagc ggtacgtcgc
     2641 cgagcacccc gggaccttct tttaccatcg cccttcgtat cgcgatcaga cctggatacc
     2701 cgccacgctg gaatacggcg tcctggtcga tggggagcgt tggtgggtgg atcggttcag
     2761 gaacgtctga gcgctgcgcc cggcatgcag gctccgcgct agcgtgccga cagcacgatc
     2821 gccccggggg cgaaccagag ctcgtaatgg cgtgcgcgga tcgaaccgct gctgccgtag
     2881 agcgcgctga aatcctccag gctcggcgtg ggcgactgca gcagcgcggc gagcgcacgc
     2941 gcgagggcct cgccgtcctg gcggcattcc tcggtacgga agcagcgcag catgctgcgc
     3001 gcgggggcgg ccttgaccag cacgatgccg gggagctcga gcgcggcgtc gccgatgcgc
     3061 acactgcgca ggcgcgcctc cagcgcctcg gcggtcgggc gctggccctc ctcggcgatg
     3121 tagatccaca gccgcggtgc cacgccgccg gacgccggag cgggcgccgc ggagaccggc
     3181 ggcgcggcct gctgcgccga gagattggag agattgctca cctgggcgcg cagctccgac
     3241 ttggcctcgg tgaggctgcg ctgcaccgcc tgtccgtcgc cgctcgcagg ggcgttcgcg
     3301 gcggtggtgg cgcgttcgag ttcgtcgacc acgctggcga gcgcggtggc ggattgccgc
     3361 gcctcctgcg ccaccgcctc ggccgcagcg cgtgcggcgg cctcggcctc gcgcgcctcg
     3421 accagggcct cgagctggcg caccacctcg tcgcgtgcgg tgagggcgag ctcgcgctcg
     3481 ccttcggcga accggcgcgc cgcctcggcg atccgcgact gctgccaggc gagcccgccg
     3541 agcgcgctgg tgaaggcgag cacgctgagg cccacggcga gcgcgcggct gcgccggcgc
     3601 aggcggcgtg cctcctggcg ctgcttttcg gcgacctcgc gctcggccgc gcgcagggcg
     3661 cgcgcctcga cgctggcggc gagcaggcgc tgcacgcgct cccagccgcc accgtagcgt
     3721 tccgcccagg cggcgttgcg ccggcgcagc cagcgttcgc gctcgtcggt ggtggtggcg
     3781 ccgagcacgt tgcccgggtc gcgctcgaag ctgtcggcct gcaccagcag cgcgcgccac
     3841 accaggccgt tgcggaactc gcgcgccagc cagccctcgc gcggctcggc gatgcgccgc
     3901 cagcagcgga tcagggcctc atggctgatg tcgatgagct cgtcggcggg gagcgcctcg
     3961 tgcccgtagg gctcgaggaa ggacacgccg tcggcacgga aagtgtccac cacgcagcgc
     4021 agtacggact cctcggcgcc ggtcaccgcc accaggcgtg cgagcgggca gggccggcgg
     4081 atggcctggc cctcggcgtt gatgtcggtg agcgcgcgga agaggtcttc caccacccgc
     4141 gtcgcctcgc cgccgaggca ttgctgctcg gcgcgcgcct gcacggcgtc ggcgtgtgcc
     4201 gagagcaggc cggcgaggcc gtgctcggcg tggtaatgct ccaccccgag ccgccacgcc
     4261 ggcgcgcccg gggtcggtcc ggcaagggcc aggcccgcgg cacgcgcacg ctcgtcgtgg
     4321 agcagcatca gcccgtgctg gatcagcggc agctggtcct gtccgccgcc gccgtccacg
     4381 atcaggcgct cggcgagctc gcgcgtcacc tcgccgtcgt agagcaccgc cggctcgcgg
     4441 atcgcccgca tgaggtcgtc gtgctccatg cgcggcagca ggtattgcgt gcgattgacc
     4501 gcctcggcga agccctcgta gcgcgagcat gcgccgagaa actccgagcg catggtcagc
     4561 accgcgtaca gcccggccgg cggcgcatcg agcaggccga ccaggcaggc ggccagcagg
     4621 gtcgcttctt ccgcaccatg gcggcgggcg tgctcgaaga gctcctcgaa ctggtcgatg
     4681 aggatgcaga cctggctgcg cgggccgcag ccgagcagct cggcgagcgc cgccggggct
     4741 tcgcggccga agttgagcgc gcggcggaag gcgatcgtgc gcagctccag cccgtgcccg
     4801 tcgccgccgg gcgtgtcgag cgcggcgagc gcacgcgcga ggttccacag cggcgcagcg
     4861 cgcggcagtg tggtgcaggt acgccagcgc acgccgccgc gcgcgctctc ctgctccagc
     4921 cgcggcagca cgccggcgcg caccagcgag ctcttgccgc agcccgagtc gccatggacc
     4981 acgagcaggc gatggccgag caggcggtcg acgatctcgt cggtcatgcg ctcgcggccg
     5041 aagaagatcg gccactcatg ttgctcgaag gggcgcaggc cgggataggg gcggggcggc
     5101 aggcggatcg cgaagaccgc gtcgaaggcg tcgtcggcgg gggcaggcgc gccggggttc
     5161 atggtgcgcc cccggcctgc aagcccttgc cgcccagcca gtcgcgcacc gcgaccggcc
     5221 agcctgcgtc ggtgccgtcg atccagtccg cctgcacgat gcgcgcggtg gcgcggcgca
     5281 cgttggtggc gatggtggcg ccggcggtgt cgagcaggcc gcagggcagc agtcggtgag
     5341 agcgtgcgcg cgccgactgg cgatcgtgct tgcccaccac cacgaggtcg gcatcgaggg
     5401 cgcgcccgtc ctcggtgccg agcagcagca gggcgtcgca gccgctcagg gtctccaccc
     5461 tgcgctcgcg cacggcctgc aggcgcagcg gatcgctgtc gacgcggtcg ggctcgcccg
     5521 gcaccaccgc gaagcccttg tcgaccagct tgagcgccgc ctcttcccaa gccgcggcgt
     5581 ggtcgctgcg gccgtgcagg tagagcagct ggccgccgtc gcggaccagg cgctcggcct
     5641 cctcgcgctg gcggcgcagc tcctcggtgc gcgcgcgcat cgcctcgagc ttggggtaga
     5701 gccggccgac catgtcgagc agggccttgc ggaactcgct gccgaaggcg cccggcaggt
     5761 cggtccagcc gagcgggcgc ggcggcacgc cgtcggccgg gtagaaggcg aagccgacca
     5821 gcggctcgcc gcgcgcatcg cacaacgccg ccggccacgg tgtgtcggtg ggccagatgc
     5881 gcaccaccgc gatacgctcc tcgtgcggca ggccgagcgc ggcctggcgc tgcaaccacc
     5941 agtcgcgctc gtcggcgcac cacttcgacg cgaggtagtc gggcgacatc agcaccacca
     6001 gcagcgccgc gccggcgatc tgctcctgca gctgggcggt gagcggtgcc atcgggtcga
     6061 ggctctgcgc cgggcggtgg tcctggtcgc ggaacacgct caggctggtg cggaagcggc
     6121 ggtcggcacg cagctcgcgc tcgagctcgt gcgcgaacgc ggccgaccac ggcgcgaggc
     6181 aggcgccgcc gctaccgtcg tcgccgtggc tgtagctcac gaagaggtcg tggcggaagg
     6241 gctctccgac ataggacatg gggactccct cgcgcgggcc cgtgcatcgc cgcagcccgt
     6301 cccgcgtggg tgggccgccg ggcggcggcc cctgctttcc ttatagtcca cgcgccgggg
     6361 ctgggccaca cgggctgcgc ccgcgccacc gcggcgctca gtcgtggcga cccgaggcca
     6421 gccgctcgat catcgcgcgc acgctgcgga tctggatgcc gtcgcgggtg gcgtagtgcg
     6481 cgtcggtgta gccccaccag tcccagcaac cgttggggtt cttccacggg atcgacaccg
     6541 cctgcgggaa cagcaccacc aggcggttgg tgtcggccca gcggtcgtag ccggtgttgc
     6601 gcacgaaggt ggtgccgttg tacagcccgc catcgggcgg ccgccgcaag ggcaggaagc
     6661 tctgcccctg cttgcagccg tggaacacca cgtgcagccg gcagcgctcg cccgccgcgc
     6721 aggcgggcgg gatgtagacc cagccggtct tgtccaggcc ggtgccccac agatggctga
     6781 agaagccggt cgcggggatg taggcgcgct ggtcgaactc gacgaagcgg cccgccggcg
     6841 tgggcgtggt gcgcggcggg gtgacgtcgg ggccgtagat ccactcgagg atcgcgtgcg
     6901 cggcctcgaa gccgcagctg ccgatgtagg gctcgtcggt cgtgccgcag gcgccgccgt
     6961 aggcggtggt gggcatggtg tggccggcgg cgtcgagcgt caccggcttg agtgcggccg
     7021 cgggcaggcc gaaggcgtcg tagtagccgt gcagctggcg ggtgatcgcg gccggcacca
     7081 gggcatcctt cccgcccacc acggtcacca cgcgctggcg ggcgatctgc gccgggtcgt
     7141 cgatgcggcc ctcggcccgg aaggcgcgcg tggccgcgac cagctcgggc accgccgtgc
     7201 tggcctcgcc gaccttgcac gacagcgtcg gcgcgccggt gcaggagcat tcggtcgtgg
     7261 ccttgagcac gctgtcctgc gcacagtagt agggcccgcc ggcgaccacc cccgcgccgc
     7321 ggacgatccc ggagtgggcg acctgcatct gcaccgacat gaagccgccc gaagagatgc
     7381 ccgacaccga ggtgtcctcg atggcgatgt tgagcgccgg cagcgctccg gggggcgcgg
     7441 cgagggcgat ggcggcgacg gcggccgcgg cgaggctggc cgcggcgcgg aggagggagg
     7501 aaagagggtg caggcgcttg ctcatgacgg acctccggca ggcggcgtgg gggtggattc
     7561 gtgtccagca tagtcgagcg cggcgcatcc gcgggagctg cgcgacggct atgcgataat
     7621 ccgcggtctt cccgcttgtc ccgccatgtc cgtttccctg cccttcgccc cgcacgccct
     7681 tccgtcctca cggctcgccc ccatcggcgc cgctcccgcc cgtcgaggcg gggcgtgacg
     7741 ccgccgcatc ccgcgccatc ccgcccgggc acgctcgcgg tttggtgggc ggcgatcagg
     7801 ccgcgcacgc tgggcatggc gctcgccccg gtcatgctcg gtaccgcgct cgccgtcgcc
     7861 gacggcgccg agctggcgtg ggcggtgctg ttcgccaccg tgtcctgcgc gctgctgatc
     7921 cagatcggca ccaacctcta caacgacgcg atcgacttcg agcgcggcac cgatcgcccc
     7981 gagcgcctcg ggccgctgcg ggtcaccctc gccggctggg cgagcgccgc cgaggtcaag
     8041 cgcggcgccc tgctctgcct gctgctcgcg ctcctgctcg gcgcctggct ctgtgtcgtc
     8101 ggcggcgccc tgatcctcgc gatcgggctg gcctcgctga tcgccggctg ggcctattcc
     8161 ggcgggccac ggccgatctc gcactcgccg ctcggcgagc tcttcgtgct gctcttcttc
     8221 ggcgtgttcg cggtgctcgg cagccactgg ctgcagtcgc gcgcgctcgg ccacctcgac
     8281 ctgctcgccg ggctggcggt gggtgcgccc gccgccgcgg tgctgctggt caacaactat
     8341 cgcgacctcg ccggcgacct gcgcgccggg cgtcgcaccc tcgccgcgct gctcggcgcg
     8401 gagggcgcgc gccgcgcctt cgcgctgctg gtgctgctgc ccatcccgac gctgatcctg
     8461 atcgccgcga gcggcgcgcc gggcgcgatc ctcggcctcg tcggcgcgcc cgcgctggcg
     8521 ccgctggtgt ggcgcttcgg tcgtgccgag ggcgggccgg cgctcaacgc cctgctggtc
     8581 gacaccgcgc gtgccggtac gcgcaccggc ttgctcgccg cggccggcat cgtcttcgtc
     8641 tccagcctgt agatcgcacc gatgagcatc gacgtcctgc tccccgccgc cctcgccttc
     8701 atcatgttct cggtcgggct ggcgctgcag ggggcggatt tccaccgcgt gttcgcacgc
     8761 ccgcgcgcgc tgctgatcgg cctcctcggc cagctcgtgc tggtgccgct ggccgcgctc
     8821 gcggtggtgc tggccttcga cctgccggtg ctcctcggca tgggcctgat ggtgctggcg
     8881 gcctgcccgg gcggggcgag ctcgggcttc ctgacccacc tcgcgcgcgc caacgcggcg
     8941 ctgtccttga gccttaccgt gatcagcagc ctggccgcgc tgctgacctt cccgctgctg
     9001 gtcaaggcgg tgctcgccgc attcggcgag ggcgtcttcg gtgcggacgg cagcgtgctg
     9061 gccgagctgc cggtcggccg cctgatcggc agcgtgctgg tggtgacgac agtgccgatc
     9121 gtcgccggga tgctgctgcg gcgccgggcg cccgcgctca cggcgcgcgc cgagccgctc
     9181 gtcgcccgcg tcgccacgct gttcttcgcc gccatcgtgg tcgccacctt cgtgtcgcat
     9241 cggcacacca tcctcgccaa cctgctgtcg gtgggaccgg cgaccctggt gctcaacttc
     9301 gtcgtcatgg gcctgggcta cggcctggtg atgctggcgg gcgccgagcg tcgggacgcg
     9361 gtcgcggtgg cgatggagtg cgggctgcag aacgcggggc tggcgatctt cgtcgccatc
     9421 gtgctgctgc gtcagcccga gctcgccgtc ggcgcggtgg tgtatgcgct gacgatgaac
     9481 ttcggcgcgc tcggcctggt gttcgtggcg cgccggcgcg agggcgcgct cgcgcacgac
     9541 gccaaacgag tcctgcggct gcccccgggg cctccgacgg gctctcgcgc cacgccgccg
     9601 cggcgttgaa cacccacgtt ccgcgccggc gccggctctg cggcgtgcga tacggcctcg
     9661 atcggggcgc gtgcatctgc acgccgtatt ccgctaacat cgggcagttg atgaccggcg
     9721 gctgagatcc gccgccccga ccggccctcg atgtcgacga gcgatctgcc ccgcaagccg
     9781 accggcgtgc ttgccgcaac cctgttgatc ctgttcggcc tggcgctttt tgtcgcgctg
     9841 gcctggtcca gccacgccag cctgcgcgaa ctcgaagcgg tcaccgatgc gcgctcgcgc
     9901 tcgcgcaacg ggctgctgct ggccgccgag gcgatgtcgg cgctgaagga cgtcgagacc
     9961 gggcagcgtg gctacgtact gaccggcgac gagcactacc tcggtccctt cgagaccggc
    10021 acgcgcagcc tggacgagac cttcgaggcg ctgcgccgga cgctcgacga cctgccgcag
    10081 gcccgcctcg agaccctgtg gaccctcgtc gagcatcgcg tcgcgctcgc cgaacgcaac
    10141 atcgccgacc gccgcgccta cgcggaagaa aattcgccgt ggaagcagcg cctcgacgag
    10201 ggccgcgaca ccatggatcg catccgcgcg cgctttgccg gcatcgaggc catgctgcgc
    10261 gccgagatcg aggcgcgcga tcgcagcttg tcggcactgc gggcgcacgc gctgcttctc
    10321 aacgtgctgc tgccggcaat cggtggcgcg ctgattctcg ccgcgtgggg gctgctgcag
    10381 cgcgagcggc gccggcgcgt cgatgcggag gcggcgctgc tcgccgcgaa cgcccgcctg
    10441 gaggacacgg tcgcgcagcg caccgtcgag ttgcagaggg cgctcggcga gatcgaggcg
    10501 ttcgcgcacg gcctcgaccg cagcatcgag gacgagcgca ggcggctcgc gcgcgaggtg
    10561 catgaccagc tcgggcagct gcttaccgcg ctcaagatga tggtgcaccg cgccctccag
    10621 ggcgcgcccg gacgggagca ggactggccg cagatcgagg ccgtgctcgg cgagggcatc
    10681 gccaccgtgc ggcggatctc ggcggcgctg cgcccgccgc tgctcgacga ccttggcctg
    10741 gaggcggcgc tcggcctcgc cggccgccag ttcgccgagc gcaccggcct ggcctgcgag
    10801 gtcgcgctcg tcgatgccgg gcagctgagc gccgaccagg ccaatcagct ctaccgcatc
    10861 gcacaggagg cgctcaccaa cgtcgcccgc cacgcgcagg cccgtcgggt gcgggtcgag
    10921 ggcgtggcgg tcgacggcga gtatcgcctg gcggtcgagg acgacggcgt cggcatggcg
    10981 ccgcaggcgc gctcctcgct cgggctgctg agcatgcgcg agcgcgcggc gctcgccggc
    11041 ggcgagctgc acgtcggctc cggcgccggg ggcggggtga ggatcgaggc gagggtgccg
    11101 ctgatcgcga ccggggggga aggaggatga agctgctgat cgtcgacgat catccggtga
    11161 tgcgcttcgg cgtgcgccag ctcgtgaccc aacgctggcc cgacgccgtg atcggcgagg
    11221 cctcctgcct ggcgcaggcg ctcgaacagg tgcgggcggc ggaatgggag ctcgccgtgc
    11281 tcgacctgtc gatgcccgac accagcgggc tggaggggct cgtgcggctg caccgtgccg
    11341 caccgctcac ccggctgctg gtgctgagca tgcacgacga ggcggcttat gcggcgcgtg
    11401 cgctgcagct cggtgccgcc ggctacctga ccaaggagca cgcgaccacc gagctggtcg
    11461 ccgcgatcga gcgcgtgcgc ggcggccagc gctacatcag ctccgacctc gccgcccgcc
    11521 tcgccgatct gctctcgggc gaggcgccgg cgcgtgcgcc gcacgaaacg ctgtcggcgc
    11581 aggaataccg cgtgctgctg ctgatcgccg ccgggcacgg cgcgagcgag atcgccgaga
    11641 tcatgcatct gtcggtgaag acggtgggca cctaccgcag ccgcatcatc gacaagaccg
    11701 gactcgccaa tacggcggag atggcgcgct actgttccga gcacgggctg gtggatcggg
    11761 ggggctgagg gcgcgcgggg ggaggttttt cgggcgaggt gtcgcgagcg aaccggcggt
    11821 ggggcgggac ttgcggtcgc cgcctgcact cgcggcccgt cgctttcgcg ccgggctgcc
    11881 ctcggcggag cacgtcgcgg gggcggcgac gcaaactcgt cgctgcgctc ctcggacatg
    11941 cgtcgccttg tttcccccgc gacgccctcc gcctcggcgc tcctgaagtt ggcgaccgca
    12001 agtcccgccc cgccgcctgc agcgtgcagg ctgcggtgtc gtcgggtgag gctgcagtgc
    12061 cccgctgccg atgttgtagg tgttcgccct acagcatgat cggcgggccg ccgacccctg
    12121 catcggcgag gcgccgctgg gggcgagcgg cgcggacgcg gaacatggat gacgtccgat
    12181 ccctccgtgg aaccgccatc atgcgcatcg tcatcgtcga agattccccg ctcctgcggg
    12241 acaacctgat ccgcctcttt gccgaccatc ccgagctgag cgtgctcggc agcgcggccg
    12301 gcgaggacgc ggccgtcgcg ctgatcgcgg cgcagcgccc cgacaccgtg ctgctcgacc
    12361 tctcgctctc ccagggcagc ggccttggcg tgctgcgccg gctgcgcgcc accccgggcc
    12421 tggcgccgcg tgtgctggtg ctgagcagcc ggcctgccga cccctgggag gcgctgtgtc
    12481 tcgacgccgg cgcggatgcc ttcctggaca aggccggcga ccccagcgtg ctgctcgccc
    12541 agctcgcgag ctggatgccg ccgctgccgc tcgacgagct gcgccgccgt cgccggctgc
    12601 agcagctgca ggtgctcgat acccgggccg agcccgcgct ggacgcgatc gcccgcctcg
    12661 ccgcacgcat cgcggaggcg ccggtcgggg tgatcagcct ggtggacacc caccgccagt
    12721 ggttcaaggc gcgcgtgggc gtcgaagtgc agacgacctc gcgttcggtg agtttctgcg
    12781 cccatgcgct gctgggtgac ggcctcttcg aggtcgagga cgccaccctg gacccgcgct
    12841 tcgccgacaa cccgctggtg cgcggcgacc cggggatccg cttttatgcc ggcatgccgc
    12901 tggtgatgcc gggcggcgag gtggtgggga cgctgtgcgt gatcgactcc cggccgcgcc
    12961 gcctgggtgc gacggcgcgc gaggcgctgg cggtgctggc gcgcagcgtc accgacgagc
    13021 tcgagctgcg ccagcgtgtg ggcgagctcg aggaggaggt cgcccgccgc cacgaggccg
    13081 aggcgcgcat catgcaactc gccacccgcg atgcgcttac cgggctgccc aaccgcgccg
    13141 cgttgatgga ccggctgcac cagggggtac gggccgcgca gcgcagcggt aacctgctcg
    13201 ctgtgctctt cctcgatctc gatcgcttca agtggatcaa cgacacgctc ggccacgacc
    13261 tcggcgacgc cctgttgcag gagatggcgc tgcgcctgag ccgggcggtg cgcgagtgcg
    13321 acaccgtcgc ccgcctcggc ggcgacgagt tcgcgatcgt gctcggcgat ctctggcagg
    13381 tcgcggatgc ggaggcggtg gcggccaaga tcgtggacgt catcacccgg ccgctggagg
    13441 tgcgcgggca tgccctgcgc gtgggcggca gcgtgggcgt ggcggtgtac ccgttccagg
    13501 cccacgacga ggagtcgctg ctgcgccacg ccgacctggc gatgtaccac gccaaggagt
    13561 cgggcgggaa ccgccaccag ctcttctccg agcagatgaa cgcgcgcgcg gtcgagcgca
    13621 tgaccgtcga gtcggagctg cgcgccgcca tcgccgccgg ccagctcgag ctgcactacc
    13681 agccgcagct gtgcctcgaa gacggccggc ttgccggcat ggaggcgctg gtgcgctggc
    13741 gccatccgcg cctcggcctg ctcgcacccg gccgcttcgt gccgctcgcc gaggactgcg
    13801 ggctgatctg ggagctcggt ctcgcggtcc tggaactcgc gctggcgcaa ctcgcggcct
    13861 gggaccgggc cgggctggag gtgccgcgca tcgcggtgaa tgtgtccccg gcgcagctgc
    13921 gtccggagct ggtcgagcgc atcgtggaga tcctcgaccg acacggcgtc gccgcgcgcc
    13981 ggctggagat cgagctcacc gagagcgcgc tcaccgccga cggaccggcc gccaacggcc
    14041 tgttgcaggc gctgcgccgc atgggcgcct cgatcgcgat cgacgatttc ggcgtgggct
    14101 actcctcgct gaccctgctg cgccggctgc cgatctcggc gctcaagatc gaccgcagct
    14161 tcgtcgccga gctcgccacc aaccgccagg acgcggccat cgtcgaggcg atcctgcgca
    14221 tggcgcacag cgtgggcctg cgcaccgtgg ccgagggcgt ggagcatgcc gaacagcagg
    14281 cggcgctcgg ggagctcggc tgccaggagg tccagggcta tctgtgcgcg cgcccgctcg
    14341 aggcgaaggc ggcaagcgcc tggctcgccg ggcagcatgc gggcgggttg gtgccgagcg
    14401 ccgcggcggc gtgagggggc gccggcttgc gtcggaacgc ggctgcggcc gccggttcag
    14461 tgcgctgcag gcaggctgcg gggggcgacg acgaccgggg tgggcgccgc gtgcgtgcgg
    14521 tgcggcgtac cgtggagcag ggcggcctgt tcggcgaggg tgaagttgcc gtactgcgag
    14581 tcgtgggtga tctcgtcgcc gatccagtgc ggcaggcaga gcggggtttc ctcgtgcggc
    14641 agttcgacct cggcgaccac gaggccggcg tgatggccct ggaagacatc gacctcgaac
    14701 acgtggtggg cgtggtcgac caggtagcgg gtcttgcgca ccacccgccc ggcgcagtgc
    14761 cgcacgatca tctggtgggc gtcgtccacc gggatcgggt actcgaattc gtcgcggctg
    14821 aagccctgct tgggcgactt cagcgtcagg aaggcgcgct cgccgcggat gcggactcgc
    14881 gtcgacatgg agccgacctc cttggcgagg tagccctgga tgatgggctc gcctctgcgt
    14941 ccgtcgagga tccggacgtc gcggacgagg aacctgcgct cgatctcttg cgccatggct
    15001 ggaaaccctg ttgcagaagg ttcgaattgt agcaaaggat tgcggcgtga ttgttgcggt
    15061 gcaatatttc tcggggcctc gattgcggac cttcccgtgc aggacgttgt tgcggcccag
    15121 ggcggtcgca tgaccggacg ccggctccca ccacgcgttc acgggacgcc tctggcgggc
    15181 cacggcgggc ggccgattgg ctagactgtc ggccatcacc tccacgggat ggaccatgag
    15241 cgcacccacc ctgatcgcat tgatttccca gaagggcggc tcgggcaaga ccacggtcgc
    15301 catgcagctc gccgccggcc tcgccctcga gggctaccgc gtcgcgctgg ccgacctcga
    15361 tccgcaggag agcgcctcgc gctgggccga gtccgcgccc gccgatgcac ccttccccgc
    15421 ccgcgtggtg cgcctgtccg gcagtgccga cgagatgggc aagaccttga gaccggttgc
    15481 caacaaggtc gatgtggtgg tcatggactg cccgccgtcg atcgagcatg cgcacacgat
    15541 gagcgcgctc gcgctgtgcg acatcgccct ggtgccggtg gtgccgggcc cgaccgacct
    15601 gtgggcgacg cgcggcatcg agcgcctgat cctcgagcgc aagcgcagcc gccccggcct
    15661 gcgcggcgcg ctgctgccca accgcgtgac gcgtaccgcg ctgtcggcgg acgtgctcga
    15721 ggtgctgcgc gacttcaccc tgccggtgct cgacgccgcg ctgtcgcagc gcagcgccta
    15781 tgcgctgggc gcggtgcgcg ggacctcggt gttcggcctg gggcggtccg ccgcgcccgc
    15841 gcaggaagag gtggcgcgcc tggtcgcggc ggtcgtcaag ttgatcgagg agcgaggatg
    15901 agcgccacca gcagcggact gaagtcgaag ctgcgcgaga ccctgaagca ggaggacgcc
    15961 gcgctcgccg agcgcagccg ggcggtcgcg aagccggctg cggcgcagcc tcgtgtgggc
    16021 aagccggacg ccgcggagcc tgccgccacc gcgacgccga cgaccgccgc gcaggcgctg
    16081 cagccgacgg tgggtggatc cgctgcggcg gagaagcgtg ccacggcaac gctgctcgag
    16141 cttgccgcgc ccgccgcggc cgggcgccgc gacaagggcg agaaggtcga gcgcgattcg
    16201 ttctcgatgc cggcgcgcga gcacaagcgc atcaaggcgt tgcgcgaggc gctcggcgag
    16261 cacggtgtgc tcgtgagcaa gtccgcgctg ctgcgcgccg gcctcgcgct gctggccgag
    16321 cgcgacaccg gcgagatcgc ccgcctggtc gcggccctgc ccaaggtggc gaagggcaag
    16381 cgcaagaggc gctgacgcgg gaaccgcgat ggtgcccagg gagcagcgcc gcaaggcgcg
    16441 aaccgggctc ccgggagact tcgggcctgg gctcgggcgg gagcgcggcg gtcgcagcgc
    16501 agcggatttg cgcacacgcc tgcgcaagcg cggtatcgcg actcgcgtcg ctcctacagg
    16561 ccctgcggaa gccgcgtcgg ttttccgtcg gcgcgacgcg cgtcgcgatt ttcggcgcct
    16621 gcgaatccga gcagccgcaa ccagtagcgc agtccggccg gccaggtgtc cgcggccgcg
    16681 cgcagcaggt cggcggtcga cacgtgggtg gagaaggcgc gcgagtcggg ctcggcgagc
    16741 aggcgtgccg gccactcggc ggcgaagcgc tgcaggccga ggccgagcag cggcaggtcg
    16801 gtgtcgtgac cggcgccgag gtcgccgagc acgccggcga gcgacagcca gctcttgcgg
    16861 aacaccgcga gttgctcgcc gaagccgccc gcgccctgca gcgccagcgc atcgagcagc
    16921 ccgaccagcc agtcgaagcc gagcggccgg ccgctgcgca ccagggtatc gagcgcgtgc
    16981 tcgacggtgg cgccgatcgg ggcggcgcgg gtgtcgcgca ggcccagccc ggcgaggccg
    17041 gccatgatgc gtgatgcgtc gagtgcgaga gcggcgagag cgacttccac cagggcctcg
    17101 cgctcggcct tgctcaggcg cgcggtgagg ctccagtcga gcaccgccac gcggccgtcg
    17161 ggggcgagca gcaggttgcc gccgtgcagg tcgccatgga agggcgcgtc ctcgccgcac
    17221 gaccagaagg ggcgggcgag caggccgccg acgagggcgc ggccgaggcg gcgggattcg
    17281 tgcgcggaca gcgcggcgtc ggcgagcttg gggccgtcga tgcgctccat cgcggtcagc
    17341 gcgggcgtgc accagggcag cagcgccggc acgaagacgt cggtgtcggc ggcgagcagc
    17401 gccgaggcgg cgcgcaggtt ggcctgctcg cggtcgagac ggatctcctg cacgagcagg
    17461 cggctggcgg cggcgaggtg gctgcggtag tcgatggcgg gcaggccgaa ctccgccccc
    17521 cggcgttcga ggaagtcgcc gagcgcgggc aggatggcga gctcctcggc cagccgttcg
    17581 ccgatgccgg gcttgagcgc cttgaacacg ccctcatggc tgcgcccgtc ctgccgccac
    17641 gagaagggca gcaccacggc cacgctgcct tcggcgagcg cgcggtcggc gaggtggagg
    17701 ccgtggccag cgccgagctc ggcgcggatg cgggcgtgca gggcgccgtc gtccgtgctc
    17761 gcggggaggg attcgagcgc ctgcaggtgg cggcgcagtt ccgcgtcgag gccgggctgg
    17821 cgcgcgacga cctggccgag cttgtgcagt gtgggacagc gcgcgagcag ggcggtggcg
    17881 cgcgcggcgg cggagacgct cgcggcgagc gcgagctgct cggcgacgat cgccccgagg
    17941 cgggcttccg gcagccgatc gaggaagaag gccattccct cgagcagcag cggacgccag
    18001 gcggagaggg actcgggcag cagggcgtcg aggccgagcg actcgaacag ttcggtgccg
    18061 aggtcgggat ccatggccga ggatctgcgg aagcgcgccg ggctcagcgc ttgcgggccg
    18121 cagccttgcg ctgcgaggat ttcttcttgc cggcccgggc cttctcatca tccttcgcgg
    18181 cggtgcgctt caacggcggc agcgcatcga gcagagcgat cgtttccgca tggtcgaggg
    18241 ccgcgagcgc cgcaaggccg gcgcgcatca gctcggactt gcgcaccgtc cggccggcgg
    18301 ccttcatcgt gctgcgcagg gcatcgaggc ggcgcaggtc gctggcgacc agagcgaagg
    18361 attcgcgctt gcgcttctcg ggtttcgcct tggcgggctc tgccggcagg ggtggggcct
    18421 tggtttccgg gcgggttggg ggccgtgttc cggcgatctc gggcagacgc tcgtcgagcg
    18481 cgacatcttc ctgcttcagg ctggcgcgca gggcgctgcg ggtgtgggtg ctcacggtgc
    18541 gctcctcggg ggcttcagcg ccgcattcta ggctgcgctt gcgcgctcgt gcacgtgcgc
    18601 acgggcgcgc gcaaggagat cagccgtgct cgccgcggcg caggcgctcc aggttgcgat
    18661 gccagtcggc gagcgcgtcg gcgtcgtcga gcaggtcggc gacgtgcttc ctcgttcgcg
    18721 attgccagcg cgcggtgagc tgcgcaccga ggctctcgcg gcctgcatgg aggccgaggc
    18781 ggtcgcccgt cggcgtgccg ggggcggtga gatgagcgtg cgtctgcatg atgcttcctc
    18841 ctgtcgaatc gaaaccaagc aaaaaatccg cgctgcgccc gcaattcaca ctcgacccct
    18901 cgcggctcac gcttccagca catacgtccc cggtgcatcc cccagcgcgg gatggcgttc
    18961 gtcggcggcg gggcgtgcgg ctacgagctc gccggcgcgc ggcttcagcc aggccatcca
    19021 gtcctcccac cagctgcctt cgcgcagcgc ggcgcgctcg cgccagagtg gcgcgcggtc
    19081 ggcgcgatgg gcggtggcga cacggtagtg gcgcctgggc ggcgtcaccg gcgggttgac
    19141 gatgccgagg atgtggccgg agctcgacag cacgaagcgc ttgtcggcgg cgacgtggtg
    19201 cacggtgtgg aaggtctgcg cccacggcgc gatgtggtcg tcttccgcgg ccaccacgta
    19261 aagcggctgg cggatgcggc gcaggtcgat cggctcgccc gcgacgacga gggcgtcggg
    19321 ctggacgagg cggttgttca ggtagagctc gcgcaggtac caggcgtgca tcgcggcggg
    19381 catgcgcgtg gtgtccatgt tccagtacag cacgtcgaag ggcgcgggcg actcgccata
    19441 gagccagccg tgcaccacgt agtgccagat caggctgttc gagcgcagca ggcggaagga
    19501 ggcggccatg tccttgccat cgaggtagcc cttctccttc atctgttcgc tcaggtgggc
    19561 gacgctctcc tcgtcgatga agacctcgat gtcgcccggc ttgtggaagt ccaccagggt
    19621 ggtgaacagc gtccagtcgc gcaccggcac ggcgtcctcg ccgtaatggc gcgccgccca
    19681 ggccatccag gtggcgagcg cggtgccgcc gatgcagtaa ccgaccgcgt gcacccgctc
    19741 ggcgccgctg cattcgcgcg ccacccgcac gatcgcggag atgccctcga cgaggtagtc
    19801 gtcgaagcgg acctcgcgca tcgatgcgtc ggggttcttc cagctcgtga tgtagacgtc
    19861 caggccctgg tcgagcaggt agcgcaccat gctcttcctg gggttgagat cgaggatgta
    19921 gaacttgttg atccaggggg tgacgatgac caccggctcg gcatgcacct gcggctgcgt
    19981 gggcgcgtag tggatgacct cgagcaggcg gttgcggaag atcaccgcgc ccggcgtggt
    20041 gccgaggttg tcgccgacgt ggaagccgtc gggatccgtc atgcgcacgt tgcccgcctg
    20101 caggtcggcg agcatgttct gccagccctg ccacaggctg tggccgcggg tgtcgatgaa
    20161 tcgtcgcagc gcgatcgggt tggaccagag atagttggtc ggcgccaccg cgttgagcca
    20221 cttgcgccac cagaacgccg cgcgccggcg ctccttgccc gacagcccgg gggtctcgta
    20281 gagcatgtcc tggacgtggt gggtgaaggc gagataccac tccttcacca ggtcccaggt
    20341 ggccgattcg gtccatacgg ggtcggcgaa gcgtggatcg tcggcgtgcg gcttgaccgg
    20401 gtcggcgctg ggcaggccga gcgcgcgccg ccaggtgtgc cactgcagct gccagaggtc
    20461 ggtgccgagg ccggcgaggc gctcgccgag ctcctgcgga tgcatcagcc aggccatctg
    20521 cgcatgcagg atgggcgcgg cgatgccgaa ggggtcggcg cgtgcggtca ggccctcgcc
    20581 gatcgcggcg atgtggcggt aaaccgcccg tccgtcgggg aggccgttgc ggccattgga
    20641 gaccatgcgg ccattgtggc cgttcgagtc gggcggggcc gggtgcgcgc cccggtccgg
    20701 ttgctgcttg gcagaagggg ccgtcgccgg ccgtttgccg ccccgtcgtg aagctggcat
    20761 gagaaatcgc ctccgtgcgc atcccgtgcg gagggggccg ctaccccgcc cgacgatcgc
    20821 gcgctggtcg ttgaacagag tctgcgcccg gcacttgaca gccgtattgc gctttcgtga
    20881 agcgtgtctt gcgcagcgca gcaaggtgcg gattcatcgt gacatgactg tcacatttca
    20941 cccacagaat ccttcacgaa taccgggagg atgtcatgga gaagatcgag gccgtcgcca
    21001 gcctgggaca ggcggaactg ctcaggccgg cgcgcgtgcg tgcggcgctg gccgccaacg
    21061 atcgactgaa gctctatctc agcgtgctgc aggcggcgca cgactgcgcg cagcgcggcg
    21121 acgcccgcgc gctcgacctc gcgcgcgagt tcgccgtcgc cggagtcgat gcgccctggc
    21181 tggcgcagct gccggccacc gcctaccgcg agggcaagga gctgcatgtg gccgggctcg
    21241 accggctggc cggcctgctc gccgaggacc tgtgcaccat ggcgcgcccg ctcgaaggcg
    21301 atgcggactt cgccgcgcgc tgtgcgcact ggtgcgcctg gctcgaagcg ctcgcgccgg
    21361 acacgctgtc gtcggcccag ctccacgcgc tcaccagcgg caagcgcggc aaggacgaca
    21421 gcctgcacct gctggtgatg gacctgcaca aggcgctcaa ccggctcgct tcggagctct
    21481 ccagcgagac catcgacggc gcccacgtat ggcaggtgga tgcggccgat cgctcgcgca
    21541 tcgccgcctt catgcgcggg ctcaacgcca cccgtgcgct caagctcgat catcccgggc
    21601 tggacaccgc cgccacccgc gacggtgcgc gcctgctgct gcagaacgac atcggcacca
    21661 acgacgccca cgtgctggtg attcaggtcg agggtctgcg cgtcgtgctc acctactccg
    21721 atctccaccg tcgccgcttc gccttcttcc agtcgctgct gtgcgaggtc ggcgccaact
    21781 ggagcgagcc gcaggcgcgc accagcgccg ggctcaactt cggcgccact tattacgtcg
    21841 gcaccgcgac cttcgactgt gcggacgagg aggcgctgtg cggggtgctg gagggcatcg
    21901 gcacgcgcat cgtgttcctc atcgactgga accgcgcgcg caagcggctc gaacagttcg
    21961 tcggcaagac cgacgcggtg gcgatcctcg ccgaggccgc acgccggcgc gtcggtcaca
    22021 tggcctggct ggccgccggc ggcgagcagc tggtgttcgg cgcgatgcag gcgctcgggc
    22081 cggagtactt ccgcatcggc gaccgcctcg acggcgtgct cggcaccgag aaggcccgcg
    22141 ccttcgtgct cgagagcatg gcgctggcga gcgcggcgat gcagcagcgc cagccgctcg
    22201 cgctggtcgc ggacgacacc cgcctgctgc tcgcgcgcta cctgcaccgg cacgacgagt
    22261 tcgacctgct cgccgaacac gcggcctact gccatgcgct cgccgagggt ctgcgcgacg
    22321 gcctcgccca cggccacgag cgcgaccgca aggcggcgcg cgaattctcc gagcgcgcca
    22381 aggactggga gcgccgcgcc gaccagctgg tgatggacgc acgtgcgcgc gccgaggcgc
    22441 gaccgcgctg ggaggccttc gccgagctgg tggagaccgc cgacgacgtc gccgacgcgc
    22501 tcgaggaggc cgccttcctg ctctcgctga tcgccgccga tcaccaccag ggctggcgcg
    22561 gcgaggtgca ccagaccatg cagctgctcg ccgacgcggt gctcggtgcg gcgcaggacc
    22621 acgtgcgcgc gctcgagatt gcgcgcggat tcggcgagga cagcagcgcc gaggaccacg
    22681 aggcgttcgt cgccgcgctg tggcgggtgc tcaacgccga gcgccagtgc gacgtgctgc
    22741 tgcgcgacgt gcgccgcgcg ctcgccgagc acgtcagcga cgcggcgacg ctgaacctga
    22801 gcacggactt cgcccaagcg ctcgagtcgg ccaccgactg gctgctggcg accggctacg
    22861 gcctgcgccg gctggtcttc agccgcgcgg gggtggggcg atgagcgcgc aacacaacga
    22921 ggagagtccg atgatggcgc atcccgatgc ggcgtccgcc gccgcgccgg ccgacgcccg
    22981 tcgtgtcgtc cgcctgatcc acgccgacgc gcccctgccc gccgattgca gcgccgaggc
    23041 gctcggctcc aaggcgtgga acctcgcgcg catggccgcg ctcggcctgc cggtgccccc
    23101 ggccttcgtg atcggtaccg gatggtgtgc cgaacccggc gcgctcgccg aggccgcgtg
    23161 gcggccggcg ctcgccgagc tggagcgcct gagcgggctg cgcctcggcg acacgcgcca
    23221 tccgctgctg ctttcggtgc gctccggtgc gccggtgtcg atgccgggga tgatggagac
    23281 cctgctcgac atcggcctca ccgagaccac gctgtccggc ctgctgcgcg tcagcggcca
    23341 tccacgcctg acctgggatg cctaccggcg cctgatcgcc ggctatggcg aggtggtcgc
    23401 cgggatcgac gcgcagcatt tcgacgccga cctcgaggcc gtgcgcggcg agtgtggcga
    23461 gtgcggccag ggcgacgagc gcgtgctcga cttcgcggcg ctgcgcgagc tcgccgcccg
    23521 ccacctcgac acctaccggc gcgaagccgg cgaggccttc ccgcaggatg cgcacgagca
    23581 gctgcgccag gccatcgccg cggtgttcgc gtcctggcat gcggacaagg cgcgcgccta
    23641 ccgcgcgatg cacggcctgg ccgacgacat gggcaccgcg gtcacggtgc agcgcatggt
    23701 gttcggcaac gccggcgggc tgtcgggcgc gggcgtgggc ttcacgcgcg acccgagcag
    23761 cggcgagccg cggccatggg tggatttcct cttcaacgcg cagggcgagg acgtggtcag
    23821 cggccggcgc agcgcgcacg gccacgacga gctcgccgcg gtcgcccccg cggtgtggca
    23881 ggccgtgctc gacgtgaccg cccgcctgga gcgcgccttc ggcgacatgc aggacttcga
    23941 gttcaccgtg cagcagggcg tgctccacct gctgcagacg cgtgccggca agcgcacgcc
    24001 gcaagcggcg gcgcggatcg cgctcgacct gctcgacgag ggcgtgatcg atgcggaaac
    24061 cgcgcgtgtc cgcaccgccg cgctggatgt gcgcgcgctc gccgagcacc gcatcgtgcg
    24121 cgccgacggt gccgcgctcg tgccgctcgg ccgcgccgcg agcgcggcga gcggggtggc
    24181 ctgtggcgag atcgtcttcg acgaggcgcg cgcccgtgcc cgccacgccg cgggggcgaa
    24241 gctgatcctg gtgcgccagg acgccgagac gcgcgatctc gccgcgctcg aactcgccga
    24301 gggcctgctc acctgccgtg gcgcgcgcac tgcgcacgcc gcggtggtcg cgcgccagct
    24361 cggcaaggtc tgcctggtcg gctgcgaggc cttgcgtctc gacgaggcgg ggcgcacgct
    24421 cgaactcggc ggtcacatcc tgcgcgaggg cgagctgctg actctggacg gcaacgaggg
    24481 cgcgatccat gcaggcgccg cggcggttgt cgaagtcgtg ccgcaggcgc tgctcgcgcg
    24541 cctcgacgcg ctgcacggac gcgaggtcgc ggcgctgcag tcctgaagat tttccggcgg
    24601 gagggttgtt cgatgagctt cagatccagc tacggccaca agtgcgcgat cgaactgtat
    24661 cgcgacgcca ccgagcgcca ggccaagctg gccggcgtga ccattcccac cttcatgtgc
    24721 cggcgctgcg gcaagcgcca gtcggtgagc ggacgccgcc aggtggtgcg cggcagctcg
    24781 cgctacggct atcactgcgc gagctgcagc actgcgcggc cggctggcgc gggttgcgaa
    24841 agccggccgt cccaggtgta gcggcagcgg gtgccgtcgc tgctcaggct gcgcgcgccg
    24901 cgggtgcgcg ggcgcagtcc ggctgcaggc gattgacccc gctgaggatt gcgcggatcg
    24961 aggcggtgac gatgttggca tcgataccca cgccgtagcg atcgccgccc tgtccgcctg
    25021 cctcggtgag ctcgaggaag gcgcaggcct gggcgttgcc gccgtcctcg ctgggcgcca
    25081 ccgagcgctc ctcgaagctg cgcacctgga cgtcgatgcc ggcgccgcgc aaggcgtgga
    25141 tggcggcgtc gatcgggccg ttgccctcgc cgaccaacag gtggcgggtg ccgccgaact
    25201 cgaccacgag gcggatgccc tgcgccgcgc catgctcgaa gaggtggtgc tcgacgtagc
    25261 gcaccggctc gacgttgtcg aggtaggtgc tgcggaacag atcccagatc gctgccgccg
    25321 acatctcacc gccgctggcg tcggtgacct gctgcacctc gcgcgagaac tccacctgca
    25381 ggcggcgcgg catcacgatg ccgtattcgg tctcgagcag gtaggcgatc ccgcccttgc
    25441 cggactggct attcacccgg atcacggagt cgtagctgcg gccgacgtcg gcggggtcga
    25501 tcgggaggta gggcacgttc cacggctggc cagccttttg agcggcgaat cccttcttga
    25561 tggcgtcctg gtgggagccg gagaaggccg tgaacacgag atcccccaca taggggtggc
    25621 gtgggtggat gggcaactgg ttgcagtact cgacggtgcg cgcgaccgcg ttgatgtccg
    25681 agaagtcgag cccgggcgac acgccctggg tgtacatgtt cagggcgagg gtgacgatgt
    25741 cgacgttgcc ggtgcgctcg ccgttgccga acaggcagcc ctcgacgcgg tcggcgcccg
    25801 ccatcaggcc gagctcggcg gcggcgacgc cgctgccgcg gtcgttgtgc gggtgcaacg
    25861 aaagcaccac gctgtcgcgg cgggcgaggt tgcggtgcat ccactcgacc tggtcggcat
    25921 agacgttggg cgtggcgacc tcgaccgtgg ccgggaggtt gaggatgacc ttgttctcgg
    25981 gcgtggcgcc ccactcggcg gtgacggcgt cgcagacctc ccgggcgaag tcgagttcgg
    26041 tggcggagaa ggtctccggg ctgtattcga ggcggatctc ggtgccgcac tccttctcca
    26101 tttccgcgcc gatctgcttg atcagccgta cggcggagac cgcgagcgcg agtacctgcg
    26161 gcttctccat gccgaacacg agctcgcgga aggtgggcga ggtggcgttg tagacgtgga
    26221 tgatcgcgcg cggcgcgccg cgtaccgact ccatggtgcg gcggatgagg tcctcgcgcg
    26281 cctgggtgag cacctggatg gtcacgtcgg ccgggatgtg gccgccctcg atgagcgcgc
    26341 gcacgaagtc gaagtcggtc tgcgaggccg acgggaagcc gacctcgatc tccttgaagc
    26401 cgatctcgca cacggtgcgg aacatgcgca tcttgcgctc gacgttcatc ggctcgaaca
    26461 gcgcctggtt gccgtcgcgc aggtcggtgc tcatccagat cggggcgcgc tcgatgagct
    26521 tgttcggcca ctggcggtcg gcgagcgcga tcggggcgaa gggacggtac ttggcggcgg
    26581 ggttctgcaa catcatggcg cggtcctcgt gggcatggcg tgattcgatg gacgaaagtc
    26641 tacgcgccgt gcggcggcag acgattgcgt tgttgcggtg cgagcgcgct tatttgtgca
    26701 gaaacatgcg tacttacgaa ctttaatgaa ataatatgcc tttcggaaag aatggagcgc
    26761 ccgatcgtgc aactcgaccg ctacgaccgc cagatcctgg agctgctgca gcaggatggc
    26821 cgcatcagca accaggagct cgccgaccgc atcgcgctgt cgccctcggc ctgcctgcgc
    26881 cggctgcgcg cgctggaaga gtccggtctg atccgcggct accgcgccct gctcgacgcc
    26941 aggaagctcg gcctgtcgct gatggcgctg atccacatct cgatggaccg ccacacgccg
    27001 gagcgcttct cgcacttcga ggcggaggtg gagaagatcc cgcaggtgct ggagtgtttg
    27061 ctgatcaccg gccaggacgc cgattaccag ctcaaggtgg tcgtgaccga catggacggc
    27121 taccaggcgc tgctgctcga gcgcatcacc cgtatccccg gcgtcaccgg cgtgcattcg
    27181 agcttcgtgc tgcggcgggt gatcgacaag acggcgctgc cattgccggg ggcctgagcg
    27241 ccgggtgtgc acaaactgga tatatgtcac gttgtgagcg gatagcgggg gcgggtgtct
    27301 gcgtccaaga tctccgggct gcgtggccct tgcgccgcgc tcgagttcat ccaaaatgaa
    27361 gtccgccgtg tgcggacccc caaggagacg acatggacgc ccgcgttcgc gaactggtca
    27421 aggccaccat ccctgtgctc aaggagcacg gcgttgccct gacgacgtat ttctatcagc
    27481 gcatgttccg ccacaacccg gaactgaaga acatcttcaa ccagtcgcac aacgagaccg
    27541 gcaagcagcc ggtggcgctg gcgatggcgg tgctggccta cgccgagaac atcgacgacc
    27601 cttcggtgct ggcgccggtg atcaccctga tcgccaacaa gcacgccagc gtgggcatcc
    27661 gcgccgagca ctacccgatc gtcggcaagc acctgctggc ctcgatccgc gaggtcctgg
    27721 gcgacgccgc cagcgacgag ctgatcgccg cctggggggt ggcctatggc gcgcttgccg
    27781 acgtcttcat cgacgccgag agcaagctct acaaccaggc ctgcaacgcc gatggcggct
    27841 ggagcggctg gcgcagcttc cgcatcatcg acaaggtgcg cgagagcgag gagatcacgt
    27901 ccttccacct cgtgccctgc gacggcggcc cgctgccggc ctaccgcgcc ggccagtaca
    27961 ccaccgcgcg ggtgttcgtg cccgagctcg gcgtgatgca gttgcgccaa tacacgcttt
    28021 cgatcgcgcc gggggccatg cacttccgca tctcggtgaa gcgcgagccg gcgggcgcga
    28081 ccacgccggc cggtcgggtg tcgaaccgcc tgcacgactt ctacaaggtg ggcgacatgc
    28141 tcgagctggc gccgcccttc ggcgagttct tcctcgacga gacccacgac ggccccgtgg
    28201 tgctgatcag cggcggggtc ggcatcacgc cgatgatctc catgctcgac cgcctggtag
    28261 cggcggcgcc gtcgcgtccg gtgcagttcg tgcatgcctg ccgcaatgcc gcggtgttcg
    28321 ccttcggtgc gcatctggcg aaggtggcgg agcgcaaccc gcaggtgagg ctgcatctgt
    28381 tccacgacga aggcgcggtt tcggcaccgg tgcaggcggg ccgtgtcgat ctgcgcgcgc
    28441 tggcgggcga ggtgctggca gagggggccg actactacct gtgcggcccg gtcggcttcc
    28501 tggaggcgca gatcgcgacc ctgcacgagc tgggggtcga gtcggcgcgc atccatgccg
    28561 aggtgttcga caccggcggc atcgcgacct gaggccgggg ccggctcgtc cggcttcgat
    28621 ccggccgcgg ggccgcggcc tcgcgtgtgc gcagcccggc cgcccggggt cggcgccggt
    28681 ccggtgtttt tcggtaggag cgacgcaagt cgcgattgca gtgcctggag tccgcgcctt
    28741 cgcttcggca ccagccgatc gcgtcgcccg tcgctcctgc cccgttcgcc ttactgcccg
    28801 gcgttcggga agaagagctg ctcgccctcg atcttgtacg cggcgatggc ctcctggccg
    28861 gcaggcgaaa ccacccattc gacgaacttg cgcgcctcgg cctgtttcac gtgcgggtgg
    28921 cgctcggggt tcaccaccat cacgccgtac tgattgaaga ggcgggtgtc gccttcgacc
    28981 agcaccgcga ggtcggcgcg gttcttgaag ctgagccagg tgccgcggtc ggcgagcacg
    29041 taggcgccgc tcgatgccgc catgttgagt gccgggccca tgccgcagcc gcattccttg
    29101 tatccgctcc ccttgttgtc gaccccggcg gccttccagt agcgcagctc ggcggcgtgg
    29161 gtgccgctct tgtcgccgcg cgacacgaag ttgccgttgg cggcggcgag cttctgcagc
    29221 gcgccggtga tgtccttgcc cttggcggcg gccggatcgt ccttcggccc gatcagcacg
    29281 aagtcgttgt acatgacctt gcggtgctcg atgccgtggc cttcatcgac gaacttccgc
    29341 tctgcgtcgg cgtcgtgcac gaacaccacg tcggcgtcgc cgcggcggcc gacgtcgagg
    29401 gcctggccgg tgccgagcgc gaccaccttc acgtcgatgc cggtcgcctg tttgaactgc
    29461 ggcagaatgt ggccgaagag accggactgc tcggtggagg tcgtcgacgc gacgacgatc
    29521 gaggcggcct gctgggcgat cgccggggcg gcgaagaggc cgatcgccac ggtggcgatt
    29581 ccggcgaggc tgcggcggat gcgcgcgatg cgggtgttgc gggtcatgcg gagtctcctc
    29641 gattgggggt gggctcgcgg ggtgggcggt ttcgtggccg tcgcggcggc tgcggcgacg
    29701 gatgagcctc gggcaatcta ggcaggcggg tgggatggat caatatgaag ggatctgcat
    29761 aggatggagg gccgcagcgg cgtggatgtc gccgcgcgcc acggacatgg aacagagcgc
    29821 atgaagagcc gcgtgatcga acgctgggtc gaggcccacc tcgccggacg gcgcatcagc
    29881 gccaactcgc tgatcatctc ggtgtatggc gacctgatcg cggtgcacgg cggcgtggtg
    29941 tggctggccg acctgatccg gctgatggcg cccttcgggc tcaacgagcg cgtggtgcgc
    30001 accagcgtct atcggctggt gcaggacgac tggctggagt cgggacaggt cggccggcgc
    30061 agctactacc gcctcgccgc atccggcatg cgccgtttcg cgccggcgtg gcggcggatc
    30121 ttcgagcccg agggggaggg ctggagcggg acctggcagg tggtgatgct gccctccgcg
    30181 ctcgaggcgc gggtggcgga caacctgcgc cgggagctgt cttgggcggg ctacggggcg
    30241 ctcaacgccg gcgtgctgtt gcgcccgacc gacgacgcct cgaccctgcg caacatcctc
    30301 gacggctgcg gcctgcgcga ccgcgtggtg ccgctcaccg cgcgcggcct ggacggggtg
    30361 accggcgcgg ccaccgacga gctggtgcgc gagtgctggg acctgcaggc gatcgccgcc
    30421 acctacgacg gcttcatcga gcacttccgg ccgctgctgc gcgccttcgg tgcgggcgag
    30481 gccgagccgg agcaggcctt cctggtgcag accctgttga tgcacgagtt ccgccgcgtg
    30541 ctgctgcacg atccgcagct gccggtgcag gtcatgccca accgctggcg gggggacgcg
    30601 gcgcgcgagc tgtgcgcgcg actgtatcgg ctgagctggc gagcggcgtg ccggcacctg
    30661 caggcgagtt gcgccacgcc cgaggggccg ctgccgccgc cgtcggcgca gttcttccgc
    30721 cgcttcggcg gtctggagct gccgccggcg gcgacggacg aggtcgcggc cgagggctga
    30781 gcgggcgctg tgaggcgccc gcccgcatgg ccgctcaggc gttgccgagg cggggtgcgc
    30841 tggcgaggcc ggcgggcttg ccgccgcgca cggggtgatg cttgagcgcg atgccctggg
    30901 tgaaggcggc gcgacggatc agggtgtatt tgaaccacca gccggcgccg gcagccagca
    30961 gacccgccgc ggcgagcgcg ctgcccgcgc cgggcacgcc ggcgaggatg cccgccgcgg
    31021 cgagcagcgc gagcccagcc ggcagcaggt ggccgacgcg caccatgcgc gccgcgatcg
    31081 cctcgaaggc ctgcacgctg ggggccgggg cgcgatcggc gcgcaggccg tcgaggtagc
    31141 gtctccacag cagcaggcgg gcgcacaggg cgagcacgag ggccatcgcg gcggcgatct
    31201 gcagcccgcc cgccagggtg gtggcgatca ggcccgcgcc ctcggccagt ccggtgccca
    31261 gtaccagcgg cgcgcagcgc gcatggcgcc aggcggggat gcccttgtcg gcggcgagga
    31321 tgcgcgcctg ggcgtagacg aaggcgaggc cgcacagccc ggcgagtgcc tgcgcggcgg
    31381 ggtgggcggt gtagagggcg agcacgcccg cgccgaacag cgccagtgcg accgaggcct
    31441 cgcgcgtcat ccacgagctg cggatgtgac ggaagacgtt gagcgcgcgc cacggtcggc
    31501 cgatctcgaa ccagacgcac agcagcccga gggcgacgag gagcaggccg gcggcgagtt
    31561 gcgggcgcac ctcgacaccg gcgaggccgg cgagcccggc gaacagcacg agcccgccgc
    31621 cgctgccgcc gcagatgaag ttgccggcgg cgcgccagtc ccagtgctgc tgcaggcggg
    31681 gtgcgggctg gccggggagg ggggaacggt tcggtttcat caggcgtgat cccagatgta
    31741 atagacgctc ggcccggtgc cgagctcctc gtgcatgtgg aagtgctggg tttttgcgag
    31801 caggcggctg accgcgctgt cggggtcgtc gatgtcgccg aagaccatcg cgcccgagat
    31861 gcaggagttg acgcaggccg gggtgacctc ggggtcgcgc ccggggatgc gtccgctcgc
    31921 cgcggcggcg tcgatgcgtt ccttgcagaa agaacacttg gtcgccaccg agatgcgcga
    31981 cgggtcgaag cggatcgcct cggaggcgat cggctgctcg ccgtaggcga agcgcgcttc
    32041 gtgcacgatc gagcgcgcct cgtagggaca ggccatcacg cagttggcgc agccgatgca
    32101 cacgtcgtag tcgatggtga ccaggccgtc gcggcgcttc tgcgtcgcct cggtggggca
    32161 gaccgccgcg cacggcggct cggcgcagtg catgcaggcc gtcggcagga agctgcggcg
    32221 cacgtcgggg aactcgccgg tctcgaggtc gagcacgcgg cgccactgca cgccgggcgg
    32281 ggtggcgttg gcgttcttgc acgccgcggt acaggtctgg cagcccacgc agcggcgcag
    32341 gtcgatgctc atcacatagc gggtcattgc acggtgctcc cttcgagacg gcggatcgcc
    32401 acgcgcacga tgtcggcgcc cgagccggtg gcgtcggtga gttcgagcga catcggcgcc
    32461 acggtgttga ggctgggcat gccgaaatcc ttggcgaccg gggtggccca gtggtcgaac
    32521 tggcccggga tgaccagggt gtcggggcgg atgccgtggg cgagcaccgc cttgccgtag
    32581 gtggcgccga tgtgcgagcg gatctcgacg cggtcgccgt cggcgatgcc gaggcgcctg
    32641 gcggtgttgg cgttcatgat caccccggcg tggccccgca cgttctgcgc cacctcgcgc
    32701 atcagcgcga tgctgacgtt gccgccggtg tggtactgca tgctcttggt ggcgagcagc
    32761 cacagcgggt agtcctcggg cttcgcgccg ctgtcgacca gggcctgctc ccagcgcccg
    32821 ggaacgtcgt gccattcggg cagggcggtg tattcggaga gctgggtgtc ccaccagtgc
    32881 atgtcgtgct cgtgcaggcg gttgccgagc tcgcggccga tgcgcagcag gcgctcctgg
    32941 tagggcagct cgtagcgcag gcccttctcg gccagggtgg gggtgaggta ccacgagcgc
    33001 tgcggcatcg gcacggtgta gaagccgtgc tccttgaacc aggcgaggtc gtgggtctcc
    33061 tgcccctgcg agagatcggt gctcgccgcg cggcagatgg cgtcccagat cgtgtccacg
    33121 tcgtgcgtgc gcgtggggtc gaggctgaag tcgtagccct cgccggcgag cggcgagacg
    33181 ccgccggcgc cgcggttgat cgccttgttg tagggctcga gcaggccggt gcggcgcgcc
    33241 agctcggtgc tgatccaggt gaagtcgcgc gcctcgccct gcggctcgac cgccggctgg
    33301 cgcagcacca cgccgcgcgc ggtccagaac tgctcgacga acttggtgcc gccgaccttg
    33361 atcatctgca gggattcgag gtcggtggcc tcgggcagca gcaggtcggc gaagaagttg
    33421 gtctcgtcca ccgtgtaggc gaaggacacg gtgaagggga aggtggcgat cgtctccacc
    33481 aggctgggcg tgtcccagaa cgagatcgcc gggttggagc ggtagatgaa ccagatgtcg
    33541 ggcagggtcg gcttgggcat gttgaagtcg cgcggcacct cgcgctggaa catccacgcc
    33601 agctgggtgg ggccgagcgc ctggctccat gccgagttgc cgacgatcgg caccagggtg
    33661 cggtgggcgt tgcggccggt gggccgggcg acccagtgct ccttgtcggt ggggttgaat
    33721 ttctgcgcca tgaagccgtc ctcgccagcg gcgacgctaa ggtggcggtc gtcgtgcggg
    33781 cggttcagcc gcaccgtggt gccgagggta ccgcccggca cctcgagcgc gcccaccagg
    33841 gtggcgagca ccgtgcgcgc ccagcagcac tcgaaggcgc cccagccgtt attcaccgac
    33901 ttgccgaggg tgaccgcgac cgggcgcagc ggcagggtgg cgccgtccac ctcgatggtg
    33961 gcgccgacct gcgcgttctc gaggtactcg cccgcgatgc ggcggatggt ggtcgcgggc
    34021 acgtcgcaga tgccctccgc ccactccggc gtgtatttgc gcatgtgctc gaccagcatc
    34081 gtgtgggcgg gcgtgccctc cacgtcgacg agcgcatggc gggcgtcgtc ggcatccacg
    34141 gtcaccgccg cggcgacgcg gaagcggcct tcgagcgcgg gctcgacgcc cggggtgtcg
    34201 aagggcacgg cgcggccggc ggcgggatcc cacaccagcg gcttcgcgct gtcggggtcg
    34261 cgcaggtaga ggccgtcggg gccgaccagg tagggcgagg acgtgcggtc gcgcaggaag
    34321 gggaggtcga gctgcccgag gctgtgctcg cacaccagca cgtggatcag cgcgaacatg
    34381 aaggcggggt cggtcttggg gcggatcggc acccactcgg acgcacaggc gccggtgatc
    34441 gacaggtggg gctcgacctg cacgcgcttg tagccgcgca cgcgcgcgtc ggcgtggcgg
    34501 gtgaccgcgc acgggccgcc ggaggcgtcg acgttgctgc cgatcgagat cacgtagcgg
    34561 cagttgggcg tgtccgcggc gacggtgaag gcgcggtgcc agaactcgcc gtagaggtgc
    34621 tcggaatgca cgcacttgac gccctggccg gagccgaagc tgaagtcgat cggcccccag
    34681 gcggcgagga aggcgggcag ggtgcccatg tacatcgccg gcgtgccgcc atggccgaag
    34741 ctggccgcca cgcgcggcag gccggagtcg tcgacgaggc ccttctcgcg caccgcggcg
    34801 aggcgggcgg cgatggtatc gagcgcctcg tcccaggaga tcggcacgaa gcccggatcc
    34861 tcgttgcgcc ccttcttcgg gttggtgcgc ttcatcggct gcaggatgcg gtgcgggttg
    34921 taggtcttct gcaccaggcc gtgcgccttc acgcatacgc ggccgcgcgc gggatgcacg
    34981 tcggcggcgg cgaaattggg ttcgatctcg gtggccacgc cgtcgatcac cttgacggtc
    35041 atgaagtcgg ggccggcgac gcagttgtag cagtagctcg gaatcttgcg ttcgttgcgg
    35101 gcggtggcgg cggtcatcgc ggactcctcg ctcagtgcat gcgttgcggg tgggtgtaga
    35161 cgctgtggcg gtggccgcgc gcgaagccga ccagggtgac gcccgcggcc tcggccaggc
    35221 gcaccgccat gccggtcggc gcggacagcg cggcgatcgc ggcgatgccg acggtggcgg
    35281 ccttctgcac gatctcgtag ctggcgcggc tggtcatcag cacgaagccg tcgtcgaagc
    35341 cgcagccgcg cgtggcgatc gcgccgatga gcttgtcgag cgcgttgtgg cggccgacgt
    35401 cctcgcgcac cagcgcgacg ctgccgtcgc gccggcacca cgccgccgca tgcaccgcgc
    35461 cggtgagctg gaacagcggc tgcaacgcgg cgagctcagc ctgggcgcgc tcgagcgcgc
    35521 cgggctggat cgcggtgtcg gcgaggcggg cgggcggcag cgggcgctgc aactgggcga
    35581 ggctctcgat gccgcacagg ccgcagccgg tgcgcccggc gagcgcgcgg cggcgttcgc
    35641 gcatggcggc gaagcgctcg ccggcgagcg ccatgcgcac ctcgcagccg agggcgtgct
    35701 ccaccacgtc gatgtcgtgg agctcgcgcg cgctcgcggc gatgccctcg ctgagcgtga
    35761 agccgagcgc gaagtcctcg agatcctgcg gggtggcgag catcaccgcg tgggagaagc
    35821 cgttatagac gagggcgacc ggcgcctctt cggcgaccgc gtcctcgccg aggatcgcgg
    35881 agtcggtgcc ccggcgttcg acgccgtagc tgcggtgcgc gccgcttgcg ggggaagggt
    35941 ggtcgagcat ggggacctcc tgtcgggaag gagccgcaag agggtcgtgc agcggcttga
    36001 cttaatgata caaaaaaata tcaataaatc aatatcggcg tatcacaaaa ttataaaatc
    36061 aatttaaatc agtaagttgc agatagttag atatccacga ataagattgc tcgtgttgct
    36121 tcgctatggg ggattttgca taggatgggt gcggcgggag cccgggcggt ccgtgcttgc
    36181 cgtgcgaggg gcgggcgttc agtggctgcc gaggcggtga ttcacggcgg ccaggtcgga
    36241 cggcgtgtcg atgtccagcc agctggcgtc gttgtcgaca gcgagttcga ccagcgcccc
    36301 acggtggcga gcgagcagtg cgcgtgcgcc ttcgtcgccg gcgagggcgc gcagctcgtc
    36361 accgaagcgc gcggcgaagc ccaccggatg gccgcggcgg ccccggcaga cgggggcggc
    36421 gatggcggcg ccggagcgca gcgcgtcggc gaccaggcgg tgctgggcgg tgggcaccag
    36481 cggcatgtcg ccgagcgcga ccagccagcc tgcggcatcg gcggtggcgg ccacgccaca
    36541 ggcgatgctg acgcccatgc cggggtgctc ggcgccacag cgaaccacct cggcacccgc
    36601 cgcacgcagc tcgtcctcga gcgtgccggg ctgggcggga acgaccgcca ccaccaagtc
    36661 gacggccggc agcaggcggg cgcaggtggt cgcggccacg ccgcggccgt cggccaggcg
    36721 ggcgacgagc ttgccgccgc cgaagcggct gccggcaccg gcggcgagca ggatgccgac
    36781 gatcaggccg cgcgtgcgag cgggcatgcg gcggactccg tcgccgggcg tgcgactgcg
    36841 gccgtcaggc tcgtgccgtt gcggcgtgcg gtgaggtcgg cgacgatcgc gagcgcgatc
    36901 tcgggcggcg tgcggctgcc cagcggcagg cctgccggtg catgcaggcg ggcgatctgc
    36961 gcgtcgtcga ggtcgaacag gcgcaggcgc tcgcggcggg cggcgctggt tgcgcgcgag
    37021 cccactgcgc cgatgtagaa ggcgtcgctg tgcagcgcgt cgatgagggc gaggtcgtcg
    37081 agcttgggat cgtgggcggc ggcgaggatc acgctgcggc ggtcggggcg catcgcggcg
    37141 accgcgtcgt cgggcatctc ggtggtgatc tccacgccgg gcagcgtcca gctcgcgcgg
    37201 tattcctcgc gcggctcgct gagcgtgacg cggtagtcga gggcgagcgc gaactgggcg
    37261 agataggcgg agagctggcc ggcgccgacg atcagcatgc gccagcgcgg cccgaaggcc
    37321 gcggtgagcc gggagccgtc gaacaccacc gcggtgtccg ggacggagtc gagcagttcc
    37381 acggcgcccg tggcgaggct cagccgacgt gcgagcagtt cgccgcgctc gatgcgcgcg
    37441 agcgcctcgg cgagccggct gccggcgtgc agcggctcga gcaccaggct cagcgagccg
    37501 ccgcagggta gctggaagcg ccgggtctgg tccgcggtga tgccgtaggt gacgagctcc
    37561 gggcgctcga gccattgcgt gcgcaggcga tcactcaggt cgtcctcgat gcagccgccg
    37621 gagaccgagc cggccaggcg tccgtcgccg cagatcgcca gcagcgcgcc ttcgggtcgc
    37681 ggcgaggagc cccaggtgcg caccaccgtc gcgagcgtga ccggcagtcc ggccgcctgc
    37741 caggcgacgg cctggcgcaa ggtctcgaga ttgacgctgt ccatgtcggt tcctcgggaa
    37801 aggaaaatgt tagacacgct aactattgga gttcaaacaa ttacacttcg aagtgtagca
    37861 gggtgccggg cccttgcaaa ttgaaaagca cagcagcggg acgtccggtt tcgtctcggc
    37921 caggccttat gccccggggg cgcgggctcg ccgcggacga cgcgcttgcg ctaacctcgc
    37981 acgaggccgc cacgatggcg gcagacacgg agagcgggcg gcagatgcgc aggatcaggc
    38041 tgaactacgt actcggcgcg gacacggggt cggcgccgct ccacaatccc ctgctcgacc
    38101 tcctgcaggc ggtgcgcgag cagggctcga tctccgccgc cgcgcgtgtg ctggacctct
    38161 cctaccgcca cgtctggggc gagctcaagc gctgggagct cgagctcggc cagccgctga
    38221 tcctgtggga gaagggccag gccgcgcgcc tgtccgagtt cggcgccaag ctgctgctcg
    38281 ccgagcgcca ggtgcaggct cgcctgttgc cgcagatcga ggcgctgcgc gccgacctcg
    38341 agcgcgcctt cgcgatcgcc ttcgacgatt cggtgcatgt gctgtccttc cacgccagcc
    38401 acgacgaggc gctcgccgcg ctcggcgagg aggcgcggac ccgcggcctg cacctggaca
    38461 tccgcttcac cggcagcgtc gatgcgatcc gcgcattgaa cgagggccgc tgcaccatgg
    38521 ccggcttcca cgtgcgcctg ccggcggtgc cgggctcgcg ggggtcgtcg tcacactcgc
    38581 agcgcaccta caagccgctg ctgcgccccg gcctgcacaa gctgatcggc ttcgcccgcc
    38641 gcagccaggg cctgatcgtg gcacgcggca atccgcgcgg cctgcacggc ctcgccgacc
    38701 tcgcgcgccc cggcgtgcgc ttcgtcaatc gcgcgcgcgg caccggcacg cgggtgattt
    38761 tcgacgagct gctcggcgag ctcggcctcg cgcccgccgc gatcgagggc tacgccaatg
    38821 acgaaccctc gcacgccgcg gtcgcgcagg cggtggcgag cggccaggcc gacgccggct
    38881 tcggcatcga ggcgagcgcg cgcagccgcg ggctggactt cgtgccgctg gtcgaagagg
    38941 cctacttcct cgcctgcctc aagtccaccc tggagcacga cgccacccgc gccctgctcg
    39001 cgctgctgcg caccgccgca tggcagcagc gcctggccga cctgcccggc tacgcgccga
    39061 tgcagagcgg cgaggtgctg tcgatgagcc gggtgctgcc gtggtggcgc ttcggggggc
    39121 gtggaaacgc tcgcgaagga aagcaataag ctgatgtttg aattgcaatt ttccgtgaaa
    39181 cattagcgcg gaacctggcc gctcgcgtcc gaagcgcgta gaatcctgcc ccttgttcac
    39241 gcattgcagc acggagcgcg ccatgctcta tcccacccgt ttcgatgtca tcgtggtcgg
    39301 cggcggtcac gccgggaccg aggctgcgct cgccgcggcg cgcatgggcg cggccacgct
    39361 gctgctcacc cacaacatcg agacgctcgg ggcgatgagc tgcaacccct cgatcggcgg
    39421 catcggcaag ggccacctgg tgaaggaggt cgatgcgctc ggcggcgcga tggcggcggc
    39481 caccgacgag ggcggcatcc agttccgcat cctcaacgcg tccaagggcc cggcggtgcg
    39541 cgccacccgc gcccaggccg accgcgtgct gtacaaggcg gcgatccgcc ggcggctgga
    39601 gaaccagccg aacctgtggc tgttccagca ggcggtggac gatctcaccg tggtcggcga
    39661 ccgcgtcacc ggcgtggtca cccagatcg
//
LOCUS       DAOQBS010000014        39255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_518_length_39255_cov_15.320612, whole genome shotgun sequence.
ACCESSION   DAOQBS010000014 DAOQBS010000000
VERSION     DAOQBS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 39255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39255
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_518_length_39255_cov_15.320612"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1418)
                     /locus_tag="PKN25_03120"
     CDS             complement(240..1418)
                     /locus_tag="PKN25_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HNV89918.1"
                     /translation="MNKRDFLRTLGGGLSLALLPRTLFAATTPGELPAIRLIAAWRSL
                     HDLGRDGASRGAKAEGSTDYVGILATDWQQGLARIETAVAVPDRVHGLLPDRDGGFLV
                     CANRPGPWLMRCAADGEVLARQVLEDEKSGRTLNGHAAFDPAGEWLYTTESETGSTQG
                     WIAVRRRDSLRKEAEFSTRGIEPHELLFDAEGKLVVANGGILRAAGDRKRDLERMDSS
                     LVRLDPRSGERLGQWWLKDKRLSLRHLALGSAPAAGGPALVGIALQAEHDDPAERAAA
                     PILAVWNGDSLAIPSRETVGAGYCGDIATGMDGGFYLSAERSHRVARWHPAAPARLEI
                     IAELNKAGALAELPLDGTHASLIGSPRGLGFWHPQHDPMLLRWPVEIAPDNHWTLVGA
                     "
     gene            complement(1477..3645)
                     /locus_tag="PKN25_03125"
     CDS             complement(1477..3645)
                     /locus_tag="PKN25_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNV89919.1"
                     /translation="MKAAVRSGAAQSAHAPARPLLPLSAIMLAVLSAGAQAQSTPSSA
                     KTLPTVTVNAAEEAPHDGHRATATRVGKVLQDPHDIPQAVTTVTNTLMEEQQVGSLKE
                     ALRNVSGLSFNAAEGGRSGDNMNLRGFYTFGDMYLDGIRDTAQYNRETFNLEQIDVLR
                     GSAAMLFGRGQAGGVINQVTKTPLLGNRGRITGSVGIDDYREVTGDFNKELGDTTAVR
                     LNVMKRDEGSWRSNPADGAEPEIHREGAGLSLVTGLYTDNEFTFNHYYLKTRDNPDYG
                     ISFDPATKRPTKNFSADTFWGTNNTFDDSDTSMTSLVHQYKLSSQAEIRTQLRHASYE
                     RVYWAQAPERINPPSFDGSTGSPKVRQTDTENLTLQSDLTARAELLGMKHELLAGVEY
                     LKEDSSRWGLRDLDPGAGRFYRPGAFTSAPAITYQGDTLAFYAQDTIEFVPNWKLTLG
                     ARHDSLDAEYSSLNSPQLDFSETSLRTGLSWHPNAYTHYYISYSDSFSPTADLYQLSG
                     GSYPAERSKVTELGAKWMLMEGDLALRAALYRADKEWERNTDLESTAAILTKKRRTDG
                     LELEVAGRVTPKWEVFSGFSLMDAEILEVAPGADVRLEGQRPRNTPKLTFNLWSTYAL
                     GGGWKIGGGVEAKSDRYAYVPTAANANSNFVNGKFSPNTAPGYARWDAMLAYEQKSWA
                     ARLNVKNIFDRVYYDAVYDQGGFAIPGSGRAIILTGEYKF"
     gene            complement(3875..5290)
                     /locus_tag="PKN25_03130"
     CDS             complement(3875..5290)
                     /locus_tag="PKN25_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNV89920.1"
                     /translation="MTNPSGFPLRTRLLPALLAALTAGCALTEPVVRPEQALPAQWAE
                     PARAAEPATPLHDTWWQDFGSARLDAFVTEALATTPDLRIQAERVVQAELALRQAGAS
                     LLPSLDVSGGSSTRNVDSNESSSTGIDLGASYEIDLWGRIAAGVGASRAGLMATRFDY
                     DAARLSISASVATAWFQVLALQERLDIARRNLATAERVLRVVQARYDNGAASALDLSQ
                     QRTTVLNQRKAIEPLEVQLRQTRSALAILLGRNPQAGPTPDGAGGIERLGALKVPAVG
                     AGLPSELLLRRPDLAASEARLVAAAANIAAARAALLPGISLSAGAGVGSAALLALADT
                     TRTLSISASVLQKIFDGGRLRADVDIQRSRQRELVESHRRAILAALKEVEDALANGVR
                     DTNQEAAEREILAEAERSLRLAELRYREGADGLLTVLLAQRTLFASQDQLALTRLARL
                     TAAVNLYKALGGGWSAGQAGN"
     gene            complement(5287..7302)
                     /locus_tag="PKN25_03135"
     CDS             complement(5287..7302)
                     /locus_tag="PKN25_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HNV89921.1"
                     /translation="MKIERLPDEDATSSASARAAATRPAAPPTTATPLIELSGITRSF
                     VNGEVETRVLHGIDLTIHPGEFVAIMGTSGSGKSTLMNILGCLDRPTGGTYRFMGTDV
                     AALDRDALARLRREAFGFVFQSYNLLGGASARDNVEVPAVYSGMPREERHARAEALLA
                     RLGLGERIHHRPGQLSGGQQQRVSIARALMNGGRIIFADEPTGALDSRSGAEVMKLLA
                     ELSAAGHTIVLITHEREVAEQAQRIIEIRDGRIVADPGPHPPQEPEPDFAPHIDRTSP
                     LSDVVEATRTALRALRANLFRTVLTLLGIVIGVASVIAMLAIGDGAKQKVIDQVSAMG
                     TNLLTVRPGAPNTRGRDAPATLVIEDVQAIAELPNVLASVPEQGATITLRAGNIDHRS
                     SVNGTSADYVVARNWLPASGTFFSAEDEARYATVAVLGQTVARALFPGRDAVGEFILV
                     NNIPFQVMGVMTPKGATPWGQDQDDIVFMPYTTASLRITGQRFLRNVTVAVEDVGQID
                     DTQAAVSALLLARHGVEDFQIRNMASVIDTVSETQNTLTVLLGTVAAISLLVGGIGVM
                     NIMLVSVTERTREIGIRMATGARTRNILQQFLIEALVVSAVGGLIGVAAGLGAAAIIE
                     AFGTAVQYSLTPVVLAFSCAFLTGLVFGYLPARKAARLDPVVALASE"
     gene            complement(7299..8615)
                     /locus_tag="PKN25_03140"
     CDS             complement(7299..8615)
                     /locus_tag="PKN25_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV89922.1"
                     /translation="MNEDKPSNRTARPRWGRRALLILVPLLLAGAWYGRSAFIGERDP
                     TTNYQFTTVQRGDVEDVVTATGTLQPRDYVDVGAQVSGQLRKIHVEVGDTVEAGQLLA
                     EIDPTVYRARVDASRAQLKNLRAQLKDREAQLALAQIQQRRQRALMAEDATTKESLQT
                     AEASAMSAEAQLEALRAQIEQIESTLRADEANLQYARILSPMAGTVVSITARQGQTLN
                     TNQQAPVVMRIADLSTMTVQTQVSEADIGRLKLGMEAYFTTLGGSGKRWQGKLEKIEP
                     TPTVTNNVVLYNALFDVLNPDGRLMTQMTAQVFFVVAQAKDALLIPMAALAQGQARPP
                     QAGAGPAPAAPPGAGAPAGNGMGSGRGMMSQGRGAASGTSGAGPRQGTVRVADARGAL
                     EERKVELGVSNRVQAQVLSGLAEGERVVSGLVVGNGAGRPQMTPRL"
     gene            8900..9901
                     /locus_tag="PKN25_03145"
     CDS             8900..9901
                     /locus_tag="PKN25_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNV89923.1"
                     /translation="MMKLKATSVAVALVVAGSAWAAVAVRDEPIKPIEPAKVENAAKV
                     ELGKKLWFEPRLSMSGIISCNTCHNLSMGGTDNLKTSIGHGWKAGPVNSPTVYNSSLA
                     IAQFWDGRAADLKEQAGGPIQAGVEMNMPHTLAVGILQSIPGYVEEFESVYGKNKIDI
                     DMITDSIAEFEKTLVTPDSRFDKWLRGDDKALSAKELAGYTLFKESGCVACHNGPAAG
                     GTSFQRMGVVEPYKSSSPAEGRSAVTGADADRFNFKVPTLRNVALTYPYFHDGEAATL
                     EQAVDTMGRLQLGRTYSKDEIGQIVAFLKTLTGELPQIAMPVLPPSSNATPRPDPFK"
     gene            10074..10493
                     /locus_tag="PKN25_03150"
     CDS             10074..10493
                     /locus_tag="PKN25_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNV89924.1"
                     /translation="MMNTYASSDAIACLRQAGIPVTHQRIEIARALFSAPVHLSADQI
                     WAKVLEASPDVSRATVYNTLRLFSEKKMIRELFVDPQRVVYDSTTAPHHHLYNIDSGE
                     VRDISDDELRVIGQPRLPAGVVLDAIDVIVRVRNRAV"
     gene            complement(10602..11018)
                     /locus_tag="PKN25_03155"
     CDS             complement(10602..11018)
                     /locus_tag="PKN25_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNV89925.1"
                     /translation="MAFGGFSQGETRAPQSDINMVPLIDVMLVLLIVFMITAPLLTHS
                     VKIDLPQASSAPTEQKPETVALSIDGDGRVFWNNEAVDEVALPARLSAAAAQKPQPEL
                     HLRADRNTRYEVIAEVMAEARSAGIEKMGFVTVPER"
     gene            complement(11018..11779)
                     /locus_tag="PKN25_03160"
     CDS             complement(11018..11779)
                     /locus_tag="PKN25_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNV89926.1"
                     /translation="MDSSQAFGLAHLWAQSDNIIRAVALILLVMSITSWYLILSRSLR
                     QLRARSHSGAVEAFWAAPDLDAGLRVLAERAPESPFEALAQQGAAAAQHVRRHTHSNT
                     LGGKLDTDEVITRALRKSIALSTAALESGLTVLASIGSTAPFVGLFGTVWGIYHALVN
                     ISVSGMATLDKVAGPVGEALIMTAFGLFVAIPAVLAYNGITRANRVELSELDAFAHDL
                     HAWFATGARIAAVPGAAPRAAEVATGTPAGAHGAA"
     gene            complement(11782..12543)
                     /locus_tag="PKN25_03165"
     CDS             complement(11782..12543)
                     /locus_tag="PKN25_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNV89927.1"
                     /translation="MRAPASGAEVAPRPTAAARGSSPLRRGALLGLIVAAHLGGLAML
                     ARLAGERPVPAELMPIQVALIAAPAVAEPLPAPPEPVVEPPRPPEPRPVARPPEPTPP
                     TPPMVKKPTPKPVVKKPAPVTESPTALSAAEEPAAPPPPAPAPPAAAPAATAAPATVT
                     AARFDAAYLNNPHPAYPALSRRLREEGQVTLRVLVSPDGQPAQVELRTSSGSERLDRA
                     AREAVARWRFVPARRGDSAIESWVLVPIVFKLQGN"
     gene            complement(12694..13173)
                     /gene="bfr"
                     /locus_tag="PKN25_03170"
     CDS             complement(12694..13173)
                     /gene="bfr"
                     /locus_tag="PKN25_03170"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNV89928.1"
                     /translation="MKGDKKVIQYLNKQLTIELTAINQYFLHARMYKNWGFTRLGKHE
                     YDESIEEMKHADMLIERVLFLEGLPNLQNLDKLLIGENVPECMQGDLKLELGGRANLI
                     EAIAYCESSKDYVSRELFEHILEDTEEHIDYLETQLGLVDSVGLQNYLQSQMDPGAS"
     gene            complement(13170..13409)
                     /locus_tag="PKN25_03175"
     CDS             complement(13170..13409)
                     /locus_tag="PKN25_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNV89929.1"
                     /translation="MYVCVCNAVTERQIHHAAAQGARTLRDLRHSLGVTAECGRCARC
                     AHECLRSAVPATPRPGVTALFAGIAPAFTASLEAT"
     gene            complement(13460..13654)
                     /locus_tag="PKN25_03180"
     CDS             complement(13460..13654)
                     /locus_tag="PKN25_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HNV89930.1"
                     /translation="MQPCEFPVSAAMPASRPDADSTAQRRPLLTSKELLGGRRELRIE
                     HEGSEYILRTTRNGKLILTK"
     gene            complement(13746..14153)
                     /locus_tag="PKN25_03185"
     CDS             complement(13746..14153)
                     /locus_tag="PKN25_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89931.1"
                     /translation="MLTLVLALWQAGAHAQGVVLVSAHAGGIAEVDRETAAQLYLGRR
                     TTLPDGRGVRLLDLPAGAERDLFYQRLTGKNPSQIRAYWSRMVFTGRAHPPHEAADAA
                     DALGRVLADPNALAYLPAASAANQPLNVLMLLE"
     gene            complement(14191..15393)
                     /locus_tag="PKN25_03190"
     CDS             complement(14191..15393)
                     /locus_tag="PKN25_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89932.1"
                     /translation="MSPRRTLLLLAGIAALASSPGTGAAPDLQVSGFATAGAVYSRAD
                     DLEFARVGIDAPGGNRVDTGPDSVLGVQLGWQSGDGTGAVLQVVTRETQRGDYTPRPA
                     LAFLSHALSPALTVRAGRLRSPFFMLSETADINYSQPWVRPPAEVYALNPFADMDGID
                     LLHRSPIGGSFIELHPYLGSSRIPVIGGGRANLRRLRGLTVTLERDELSFSASHAEAE
                     LAVIRTSQSYRDLVDTWRIPPDLQARLSGKGAHASFSSLGMQWDDGRWRVIAELGRLQ
                     ADAFVNSATGAYVAVGRRFGAWMPYFGLARQRQDAPLADPGLADSPQSAAAVAVFNRS
                     RNQAQRSVTLGTRWDFTPNSAVKAEFSHIETDRGAHGSFIARGNPFAPGLDDRSINLL
                     SVSVDVVF"
     gene            complement(15390..17855)
                     /locus_tag="PKN25_03195"
     CDS             complement(15390..17855)
                     /locus_tag="PKN25_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV89933.1"
                     /translation="MPPETSPSSFALRPSSIRIRLLLASTVVQVVLLSMLLANSVRLM
                     NNAMSASLETTIGQTASMLHAMATAYGEQGRLDGLEDVLGELLADADEGLLYVRIRDA
                     RGHLLVSAGMPGLEQVPEPDEQRHRFFETLLADDLVHVRRQLLLPRNQVGVLQFGVSV
                     SVLAAAREAIMQQGLAIAIAEILLTFVLLSSLGYLLTRNLRLLLEGSRAIANGFLAHR
                     VESRGKDELAVIANHFNLMAEALQNRIAELQDTAVRLRASEERHALALRGSNDGLWDW
                     DIDADDVYYSERFCQILGVQPGTLSNRPEDMLAYLHPYERDAYRQRLVEHLKGDSNHF
                     IMELRVRLPDGAYRWVLMRGVADRDENLHAYRMAGSLGDIDLRKRAEQQLVHDALHDG
                     LTGLPNRAMFIEHANRALAQRRRGDLTQIAILAINLERFSLVNDSYGHLVGDELLRRI
                     GAHIAALMRDGDVCARVGGDQFAVLLNGVESSIDALRICEQLVALPAFAPDDDGQQLH
                     PRCRVGVAISDNEREDAESLLRDADNALHKARRSERGPIEFFHTEMHAAAVRALQVEA
                     ELRKALTQGGLQVWYQPIVELANLRTTSFEALVRWPHPTLGLLSPGEFIPLAEALDLI
                     HDIGTLVLERVCADLREWAGRLPADAPLRVSVNLSARQLARPDLAAELLAAIDRVGVA
                     RERIRFEVTESVLAHPDGPAIDHLHALRAAGLDILIDDFGTGYSTLSYLHTMPCNQVK
                     LDGSFVRSLPYDQRLRAIVRHSIELAHDLGMTVVAECIEDEAQLEILRAMGCDFGQGY
                     LFSRPLDRDATFNRLHNPAPERR"
     gene            complement(17863..19245)
                     /locus_tag="PKN25_03200"
     CDS             complement(17863..19245)
                     /locus_tag="PKN25_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HNV89934.1"
                     /translation="MGGAGLVSRGLNRVRELVASAAHLGKPPDESDLERLRTQLQECA
                     EGRGGEVSTRQRAARLAETYLRLDDVGRHAFLRLIAHEFGPDPARVAQAHAAYQAAVG
                     TPRQWDAEAELRAALRSRRLRILTQFNAIPQGVKFLVDLRADLLRFIADDPLLKPLDR
                     ELETRLSAWFDVGFLELQRITWSSPAALLEKLVEYEAVHEIRSWKDLKNRLDSDRRCY
                     AFFHPRMPLEPLIFVQVALLEEIADDVQRLLDIEAPLADASRATTAIFYSISATQKGL
                     RGVSFGNFLLKRVIDDLKRDFPRLKTFATLSPMPGLVRWAQRDPEAFAEAFTATDWKR
                     LATLGIEGVDSTRLAAVLAGDPAWVEDEALAESLREPLLRLGARYLSEARRGDRPVDT
                     VARFHLGNGARIERLNWLADRSDKGLRQSWGLMVNYLYDPDRLEANLESFAANGSIAA
                     SSAVRRLARR"
     gene            complement(19367..20164)
                     /gene="lgt"
                     /locus_tag="PKN25_03205"
     CDS             complement(19367..20164)
                     /gene="lgt"
                     /locus_tag="PKN25_03205"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNV89935.1"
                     /translation="MLVHPQFDPIAFSLGPLSVRWYGLMYLVAFVLFVVLGRLHARRR
                     PELGWDEQGIDDLLMYGVIGVIVGGRLGEVLFFQPGYYLQHPGEILAVWKGGMSFHGG
                     FLGVLVAMWLYGRRHGKGFWQVTDFIAPLVPTGLAAGRLGNFINGELWGRPAETELPW
                     AMIFPWVDALPRHPSQLYQAAGEGLLLFLLLWSYSASPRPLRAVSAMFLVGYGALRFV
                     AEFFRTPDPGIFGTLSLGLSTAQWLCVPMVLAGLLMLAAARTRGTGR"
     gene            complement(20244..20921)
                     /locus_tag="PKN25_03210"
     CDS             complement(20244..20921)
                     /locus_tag="PKN25_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HNV89936.1"
                     /translation="MQDTTPTTEAGFDLDPAEIRVLGVLIEKSFITPDAYPLSINAIT
                     TGCNQLTAREPVMSLSEAEVQDAVDRLIVRRLVSKRDQQSARVAKYEHLVRLRHSLPP
                     AEQAVLAILLLRGAQTAGELRQRCERLHRFDDVAAVDAVLEHLAEKYPPLVAELPRAP
                     GTKETRHVHLLGGDAAVQEMAEAQSVGTAGGGRGRIGELEDEVRRLREELEALRGAFA
                     EFRKQFD"
     gene            21070..22920
                     /gene="ilvD"
                     /locus_tag="PKN25_03215"
     CDS             21070..22920
                     /gene="ilvD"
                     /locus_tag="PKN25_03215"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNV89937.1"
                     /translation="MPQYRSRTSTAGRNMAGARALWRATGMKDGDFEKPIIAIANSFT
                     QFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMCNAHTADALVCISNCDKITPGMLMAALRLNIPAIFVSGGPMEAGKVKWEAKVI
                     SLDLVDAMVKAADKSCSDEEVDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GTTLATHADRERLFKEAGRRIVDLARRYYEKDDASVLPRSIASFQAFENAMSLDVAMG
                     GSTNTVLHLLAAAREAGVDFTMKDIDRVSRRVPCLCKVAPAIADVHIEDVHRAGGIMS
                     ILGELDRAGLLHTDVPTVHSASLGEALDKWDIKRTEDEAVHTFFRAAPGGVPTQVAFS
                     QDRRWKSLDADREHGIIRNKEHAFTADGGLAVLYGNIAEKGCIVKTAGVDESIWKFTG
                     KARVYESQEDAVEGILGEQVQAGDVVVIRYEGPKGGPGMQEMLYPTSYLKSRGLGAQC
                     ALLTDGRFSGGTSGLSIGHASPEAACGGAIALVEDGDTIEIDIPARRIHLAIADAELA
                     RRRAAMEAKGNAAWKPVKRERVVSAALQAYAALTTSADTGAVRDVTQVQR"
     gene            22971..23165
                     /locus_tag="PKN25_03220"
     CDS             22971..23165
                     /locus_tag="PKN25_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89938.1"
                     /translation="MDTELAQLEAQLEHLISHYERLKADNAALRGRVVQLEGENRRLS
                     DKVRVAADRLAGLLDKLPEA"
     gene            23170..23472
                     /locus_tag="PKN25_03225"
     CDS             23170..23472
                     /locus_tag="PKN25_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNV89939.1"
                     /translation="MSADHLDMMLLGKEYRISCTPESRDELLATVAFLDDKLREIGAR
                     TGSSGEKLAVMTALNIAHEFLLHQRGNGFDMPGMKRRIGLMKARLDGVLGQQDKLF"
     gene            23496..23679
                     /gene="ssrS"
                     /locus_tag="PKN25_03230"
     ncRNA           23496..23679
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKN25_03230"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            23858..25315
                     /locus_tag="PKN25_03235"
     CDS             23858..25315
                     /locus_tag="PKN25_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNV89940.1"
                     /translation="MQAYFPVLGVLGMMLMLFGLVMGFPLAVSFALEDGATEAYDLAI
                     LATFATGLLMWAAGRRARRDLHVSDGFLLVAATWLITPVFGALPLLFYLPELSFTDAY
                     FEAASGLTTTGATVLTGLDTMPVSINLWRCFMHWVGGMGVIVLMVAVLPLLGIGGRQI
                     FRAETPGPMKESSLTPRIAETAKGLWGVYVVLTAGCTLALLQAGMAPWDAVIHAFSIM
                     GLGGFSTRDASLGAFDSVPIETVTIVFALLSGINYATHYLALVRRTPRPYGEDPELRW
                     FFGVLGVTVVFLTVYLLESGAYRDGALAFRHVSFHAVSLATSLGLATYDYTVWPMFAQ
                     VWILFLSSFAACSGSTGGGIKMMRAIILYKQVHREIVRSLHPSAVVPLRLGRQPVSDN
                     VLHAVLAFSFMYMVSIVSLTLILAASGLDLITAFSGVVACINNAGPGLAALGPAGNYQ
                     GLGDFQTWVMSFAMILGRLEIFTLLVVLTPAFWRR"
     gene            complement(25272..26018)
                     /locus_tag="PKN25_03240"
     CDS             complement(25272..26018)
                     /locus_tag="PKN25_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV89941.1"
                     /translation="MKSAQPPSPVVLLVEDEAGIRRFVRTALESEGCTVREAAALERG
                     RIELASRRPDLLVLDLGLPDGDGTALLAELRGWSGMPVLVLSARSDETDKVAALDAGA
                     DDYLAKPFGVAELLARVRALLRRALRPPQATACIAFGDVELDLEQRRVTRGGAPVHLT
                     PIEFRLLGLLATAAGRVVTQRQLLTGAWGPNHVEHAHYLRVYMAGLRRKLEPDPRLPV
                     HILTEPGVGYRLVPEPLSAARTPASAPPAG"
     gene            complement(26015..27178)
                     /locus_tag="PKN25_03245"
     CDS             complement(26015..27178)
                     /locus_tag="PKN25_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNV89942.1"
                     /translation="MALPTPRDRLPDRAPLRNYLLAALAAGAVALVGAPLLGQFDLAN
                     IAMLFPLAVLFAAIRLGRGPAVFAAFLAVALFDFFFVHPHFTLAVSDLQYLLTFAVLL
                     AVALTTAELAARLRRERDSAEARAEEAAQARLAADTERLRNTLLASLSHDLRTPLTAL
                     AGLAESLPLAGPPLPPAQAELAEAIRAEALRTHALARDLLDLSRLQSGAPRLALDWLA
                     VDELLGSALQARAHALRAHVFALELPEELPLLHADAVLMERVVCNLLDNAIAHTPEGG
                     HIVLAAQVGAGWIRISLCDDGCGLPPGREQAIFERFVRAPHPRGAADATTATGDAGTG
                     LGLAIVHAIVAAHGGSVSAHNRAGGGACFVLQLPLPRQPDRPPPEDEAGAPPR"
     gene            27337..28143
                     /locus_tag="PKN25_03250"
     CDS             27337..28143
                     /locus_tag="PKN25_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNV89943.1"
                     /translation="MTFDLWWLAYPVLGAAVGFFAGLLGVGGGGIMVPMLTTFFLAQG
                     FPQSQVVHMALGTSMAAIVLTSVSSLRAHHARGAVRWDVVRAITPGILVGTFAATFIA
                     ARVSTVPLAIFFAVFMAYVSIQMLLNIKPKPARELPGGLGMSAAGLGIGGVSALVAIG
                     GGSLSVPFMTWCNVKMQNAIGTSAAIGLPIALAGTVGYLVNGWGAPDMPALTFGYVYL
                     PALVLVSVVSTFFAPLGAKLAHRLPVATLKKVFAGMLVLLCAKMLHSIFA"
     gene            28178..28801
                     /gene="rhtB"
                     /locus_tag="PKN25_03255"
     CDS             28178..28801
                     /gene="rhtB"
                     /locus_tag="PKN25_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HNV89944.1"
                     /translation="MSWTMWLGFLAAALVIAVSPGPGAVLSMSTGLRYGYTAALRAIT
                     GLEIALLLQLGVVALGLGAVLATSETAFAVVKFAGAAYLIWLGVQKWRSPPEPIDGDG
                     EGVRVQSRRQLYAQGILVNLTNPKAIVFIAALVPQFIVPDRPQWPQFAIIGATMVGVD
                     VMVMSCYALLATRFRPLLRDPRLLRLQNRLFGGLFVGAGAMLAASSR"
     gene            complement(28976..29914)
                     /locus_tag="PKN25_03260"
     CDS             complement(28976..29914)
                     /locus_tag="PKN25_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNV89945.1"
                     /translation="MRFPYRLRRLRTLAACAALLLGGSVHAQALEVATSFSILGDLVA
                     QVGGERIKVRTLVGPDEDAHAFQPRPSDAREIGKAALVVVNGLGFDDWMTRLARAGGF
                     KGAVVVASAGISTLEMRRDDGHDHGHDHGKGKAVDPHAWQDVANVRRYVANIAAALAT
                     ADPDGATTYRAAAARYDGELQALDAEIRAAFAALPAERRKVVSSHAAFGYFARAYDIR
                     FLSPVGVANNAEPTAKGVAGLIRQLAAEKVPAVFIENIADPRLIERIRSESGAVVGGT
                     LYSDALSKADGPAPSYVRMMRANLATLQKALAAPER"
     gene            complement(30161..31027)
                     /locus_tag="PKN25_03265"
     CDS             complement(30161..31027)
                     /locus_tag="PKN25_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNV89946.1"
                     /translation="MFDLLISPFTEFAFMRRALAGCLALALGATPVGVFLLLRRMSLM
                     GDAMSHAILPGAALGYLAFGLSLGAMTVGGIVAGLVVVFAAGLVTRASVLKEDASLAA
                     FYLLSLAAGVMIVSLRGQNLDLLHVLFGSVLGLDDASLLLIASIASVTLASLALLFRP
                     LVMECFDPAFLRGVSRWSPVAHYGFLMLVVVNLVSGFHALGTLMAVGIMILPAAAARL
                     WTNGLPMLLLLAVLFAFGSGASGLLLSFHADVPAGPAIVLVCGVVYFVSLLAAPGGLL
                     AGRLPVRHHLES"
     gene            complement(31063..31959)
                     /locus_tag="PKN25_03270"
     CDS             complement(31063..31959)
                     /locus_tag="PKN25_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV89947.1"
                     /translation="MNRLALPMIRLDNLTLGYDRHPAVHHLSAEIPAGALVAVVGPNG
                     AGKSTLLKGLAGELRPIGGRILLPELPAGGLAYMPQRGEIDHSFPVSVFDVVAMGLWH
                     EIGAFGGVSREQRRRVRAALEAVGMAGFESRPIGSLSGGQLQRARFARLMLQDAPLIL
                     LDEPFAAIDSRTVDDLVELILGWHDEGRTILTVVHDLEQVRRHFPTALLLSREPVAFG
                     PTAEVLTPERLARARRLSEAFDEHAHECHIEELTVAVAPDAAAHPHAHDHGHAHDEGH
                     GHAHPHPAAHTAPRPRRGLPRH"
     gene            complement(31956..32123)
                     /locus_tag="PKN25_03275"
     CDS             complement(31956..32123)
                     /locus_tag="PKN25_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89948.1"
                     /translation="MKRAPPVLHGTDPQADDDTRPFPARSLLGAGLAARLALVAGALA
                     LLWATVLWALQ"
     gene            complement(32120..32578)
                     /locus_tag="PKN25_03280"
     CDS             complement(32120..32578)
                     /locus_tag="PKN25_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNV89949.1"
                     /translation="MPLHEHPAATDAARELIAARGGRVTRTRVAVIQALQRSAHPLSH
                     DELGAALDGAGIAHDRVTLYRALDWLVEQGIAQRIAGGERAWRFEIRRDGDHRHAHFH
                     CERCGQIVCLEDVSAAAPTLPAGFAVARAELVLHGACADCGREGVERKER"
     gene            32748..33308
                     /locus_tag="PKN25_03285"
     CDS             32748..33308
                     /locus_tag="PKN25_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HNV89950.1"
                     /translation="MRPILLSSVIVLALALPVAAPAQHAHGAHQHGVAELTVVLEGRM
                     LQLELISPLDNLVGFEHAPANETQRGALADAERLLLDSEAVFALPSEAGCVIREVGIE
                     SPWRDSGREHGRGQTRDHNRPARGDHEDMVASYGFECAHPEALRRLELRVFKHFPRLQ
                     RVRAEFATPRGQGGGVLTSGAAALAL"
     gene            33317..34042
                     /locus_tag="PKN25_03290"
     CDS             33317..34042
                     /locus_tag="PKN25_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV89951.1"
                     /translation="MRAHPTPAVRISGLRYRWPGSAQDCLAIDALQLGAGERVFLRGP
                     SGSGKTTLLSLVGGVILPQAGTVEVGGQALGALSAGARDRFRADHVGFVFQLFNLLPW
                     LSARDNILLACRFAPQRRARIAAAGSTPAAEAARLAARLDLGVELLERPATQLSVGQQ
                     QRVAAARALIGRPGLVVADEPTSALDADRQQTFIDLLLDECAAVGAALLFVSHDQRLA
                     AHFDRVLDLGALVRAGLAAEDAR"
     gene            34039..35334
                     /locus_tag="PKN25_03295"
     CDS             34039..35334
                     /locus_tag="PKN25_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004387345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV89952.1"
                     /translation="MSPVFMLSLRSIANRRLSAALTVVAVALAVALLLGVERLRNDAR
                     AGFAQTISATDLVVGARSGPVQLLLYSVFHIGDATANLSWASIEHVAALPQVKWLVPL
                     ALGDSHRGHRVVGTTTDFFAQYRHGEGRSLVFAAGGPFAGATARVEDLFQTVIGAEVA
                     ARHGYRLGERIVLSHGGGAGEGGARFAEHADKPFTVVGILAPSATPLDRAVLVSLEGL
                     EAIHVDWHGGAPIPGLKITPEQVRKFDLRPKSVTAALVGLHSRSAVFRVQRQINAYAG
                     EPLTAILPGATLQQLWDLVGIAERALLAVSALVVVVGLTGMVAVVLASLGERRRELAI
                     LRALGASPREVFALLALESLLLATAGIALGLGLLYGAGAALAPWLAAQHGLQPSLGWP
                     AAGEWRLLGAVLAASLVASLLPALRAYRQSLADGMTVRT"
     gene            35412..36056
                     /locus_tag="PKN25_03300"
     CDS             35412..36056
                     /locus_tag="PKN25_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HNV89953.1"
                     /translation="MNTNLHLARLVPRALRVVPLLALLAFAPLPAGAQAPAGESAGQY
                     AVGDRLAAPAPGKGEFREISWDDLIPADWNPEGFLSGLELDKLEDNDPRAAEALQRMR
                     EEWDRAPLVERMAGARVRIPGFVVPLETSGEKIREFLLVPYFGACVHVPPPPANQLIH
                     VIPDKPVPGGWNMLPVWVDGVMAVGRIDSDMGTAGYQLRAVKVEEYVEEKPKRR"
     gene            complement(36288..37070)
                     /locus_tag="PKN25_03305"
     CDS             complement(36288..37070)
                     /locus_tag="PKN25_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNV89954.1"
                     /translation="MNTRIDLDIDQGIATVTLHNPTKLNAVNAAMWRGLSAAMARIAA
                     DDSVRCVIVRGAGENAFAAGGDIEEFRSVRATVDDALHYHEDLVASALNAIRDCAVPT
                     VAAIRGACVGGGLEIAGCCDIRIAGESARFGAPINRLGFSMYPGEMAGLLALVGPAVV
                     LEILLEGRILDAREALQKGLLTRVVDDERVFEEAAVSAERIRAGAPLVARWHKQWVRR
                     LTTGVALDEEEKRRAFDFLATADYREGLAAFSEKRAPVFRGR"
     gene            37250..37558
                     /locus_tag="PKN25_03310"
     CDS             37250..37558
                     /locus_tag="PKN25_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNV89955.1"
                     /translation="MYAHIRRNPRFAELVAKRTRFAGILAAIVLIVFYGFVLLVAFAP
                     ELIGKRLFEGSNLTFGILAGLLQFIFFWVLTLVYVRRANGEFDDINKELVKAAWKEEK
                     "
     gene            37558..39237
                     /locus_tag="PKN25_03315"
     CDS             37558..39237
                     /locus_tag="PKN25_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNV89956.1"
                     /translation="MNARSLSRSAAGLLALAASPLVLAAGPAMDAAEKQPLNLHAIGM
                     FFVFVLMTLGITYWAASRTKSTADFYTAGGGITGFQNGLAIAGDYMSAATLLGLTAMM
                     YAQGVDAYIYMLSFFVGWPIILFLMAERLRNLGKFTFADITAYRLDQGKVRTMAAVSS
                     LTVVCFYLVAQMVGAGQLIKLLFGLDYNIALVVVGALMMVYVTFGGMVATTWVQIIKA
                     CMLLIGGTAVGVLAFSQFGFSFDELTNRAMEVHKLGQKLLNPGSLLADPVTAISLGLG
                     LMFGTAGLPHILMRFFTVTDAKEARKSVLYASGIVAYFFNVIAIMGLCAIIIVGQNPQ
                     FFEGGVIGGKVIGGGNMIAMHLANAVGGNLLLGFLSAVAFATILAVVAGLALAGASAI
                     SHDIYSRVIMKGKADEATELRVSKFATIGLGVVAVLLGMAFEKMNIAFMVALAFGVAA
                     SANFPVLILSMYWKGLTTKGALVGGYSGLVSAVLFVLLSKSVWVDVLGNASAIFPYTQ
                     PALFSMPIAFFMTWLFSTLDKSKAAEAEKEAFEDQYVRAQTGVGAATASSH"
BASE COUNT         5683 a        13285 c        14141 g         6146 t
ORIGIN      
        1 agcgtcccat cccccgcccg cggtggaatg ggttcgcaaa gaaaatacca acttgtagcg
       61 cgttttctcg agagccgcgg gtcgtcgtgg cggtcgtttc ccgtaagcgg gaaacgcgtc
      121 ggggcgaatt ctccgctcca tgtcgcgacg cgcgtcgctc ctgcagaaaa cccccgcttc
      181 ggagcttcct gtaggagcga cgcgagtcgc gatacggcga tccaggcggg tggcggggtt
      241 caggctccga ccagcgtcca gtggttgtcc ggcgcgatct ccaccggcca gcgcagcagc
      301 atcggatcgt gctgcgggtg ccagaagccg aggccgcgcg gtgagccgat caggctggcg
      361 tgggtgccgt cgagcgggag ttcggccagc gcgcccgcct tgttcagctc ggcgatgatc
      421 tccaggcgcg cgggggccgc cggatgccag cgcgctaccc gatggctgcg ctccgcggag
      481 aggtagaagc cgccgtccat gccggtggcg atgtcgccgc agtagcccgc gccgacggtc
      541 tcgcggctgg ggattgccag gctgtcgcca ttccacaccg cgaggatggg ggcggcggcg
      601 cgctcggcag ggtcgtcgtg ctcggcctgc agtgcgatac ctaccagcgc agggccgccc
      661 gctgccggcg cggagccgag ggcgaggtgg cgcaggctca ggcgcttgtc cttcagccac
      721 cactggccca ggcgctcgcc gctgcgcgga tcgagccgca ccagcgagga gtccatgcgc
      781 tcgaggtcgc gcttgcggtc gcccgcggcg cgcaggatgc cgccgttggc gaccaccagc
      841 ttgccctcgg cgtcgaacag cagctcgtgc ggctcgatcc cgcgggtgga gaactccgcc
      901 tccttgcgca ggctgtcgcg ccggcgcacg gcgatccagc cctgggtgga gccggtctcg
      961 gattcggtgg tgtacagcca ctcgcccgcg gggtcgaagg ccgcgtgacc attgagcgta
     1021 cgcccgctct tctcgtcctc gaggacctgg cgggcgagca cttcgccgtc ggcggcgcag
     1081 cgcatcagcc aggggccggg acggttggcg cacaccagga aaccgccgtc gcggtcgggc
     1141 agcaggccgt ggacccgatc gggaaccgcg accgcggtct cgatgcgggc gaggccttgc
     1201 tgccagtcgg tggccaggat gccgacgtag tcggtgctgc cctccgcctt cgcgccgcgc
     1261 gaggcgccat cgcggccgag gtcgtgcagg ctgcgccagg cggcgatcag gcggattgcg
     1321 gggagttcgc cgggcgtggt ggcggcgaac agggtgcggg gcagcagggc gagcgagagg
     1381 ccgccgccca gtgtgcgcag gaagtcgcgt ttgttcatcg ggatgttcca gtggaagcga
     1441 cgggcccgca ggggcccgcc gatcgatagg ctcggatcag aacttgtatt cgccggtcag
     1501 gatgatcgcg cggccgctgc cggggatggc gaagccgccc tggtcataca ccgcgtcgta
     1561 gtacacccgg tcgaagatgt tcttgacgtt gaggcgcgcg gcccagctct tctgctcgta
     1621 ggccagcatc gcgtcccagc gcgcgtagcc gggtgccgtg ttgggcgaga acttgccgtt
     1681 gacgaagttg ctgttcgcgt tggcagccgt cggcacgtag gcgtagcggt cgctcttggc
     1741 ctcgacgccg ccgccgatct tccagccgcc gccgagcgcg taggtcgacc acaggttgaa
     1801 ggtgagcttg ggcgtgttgc gcgggcgctg gccttcgagg cggacgtccg cgcccggggc
     1861 gacttcgagg atctcggcgt ccatcagcga gaagccgctg aagacctccc atttcggggt
     1921 gacgcgaccg gcgacctcga gctccagacc gtcggtgcgg cgcttcttcg tcaggattgc
     1981 ggcggtggac tcgaggtcgg tgttgcgctc ccattccttg tcggcgcggt acaacgcggc
     2041 gcgcagggcg aggtcgccct ccatcagcat ccacttggcg ccgagttcgg tgaccttgct
     2101 gcgctcggcg gggtagctgc cgccggagag ctggtagagg tcggcggtcg gactgaagga
     2161 gtcgctgtag ctgatgtagt agtgggtgta tgcgttcggg tgccaggaca ggccggtgcg
     2221 caggctggtc tcgctgaagt cgagctgcgg cgagttgagg ctggaatact cggcgtcgag
     2281 cgagtcatgc cttgcaccca gggtcagctt ccagttcggc acgaactcga tcgtgtcctg
     2341 ggcgtagaag gcgagcgtgt cgccctggta ggtgatcgcc ggcgccgagg tgaaggcgcc
     2401 cggacgatag aagcggcctg caccgggatc cagatcccgc agcccccagc ggctcgagtc
     2461 ttccttcagg tactcgacgc cggcgagcag ctcgtgcttc atgccgagga gctcggcacg
     2521 tgcggtgagg tcggactgca gggtcaggtt ctcggtgtcg gtctggcgca ccttcgggct
     2581 gccggtgctg ccatcgaagc tcggcgggtt gatgcgctcg ggtgcctgcg cccagtacac
     2641 gcgctcgtag ctcgcatggc gcagctgggt gcggatctcg gcctgcgagg acagcttgta
     2701 ctggtgcacc agcgaagtca tcgaggtgtc gctgtcgtcg aaggtgttgt tcgtgcccca
     2761 gaaggtgtcg gcggagaagt tcttcgtcgg gcgcttggtc gccggatcga aggagatgcc
     2821 gtagtccggg ttgtcgcgcg tcttcaggta gtaatggttg aaggtgaact cgttgtcggt
     2881 gtacaggccg gtgaccaggc tcaggccggc gccctcgcgg tggatctccg gttcggcgcc
     2941 atcggccggg ttgctgcgcc agctcccctc gtcgcgcttc atcacgttca gacgcacggc
     3001 ggtggtgtcg ccgagctcct tgttgaagtc gccagtcact tcccgatagt cgtcgatgcc
     3061 gacgctgccg gtgatcctgc cgcggttgcc gagcagcggc gtcttggtga cctggttgat
     3121 gacgccgccg gcctggccgc ggccgaacag catcgcggcc gagccgcgca gcacgtcgat
     3181 ctgttcgagg ttgaaggtct cgcggttgta ttgcgcggtg tcgcggatgc catcgaggta
     3241 catgtcgccg aaggtgtaga agccgcgcag gttcatgttg tcgcccgagc ggccgccctc
     3301 ggcggcgttg aacgacaggc cggagacgtt gcgcagcgcc tccttgaggc tgccgacctg
     3361 ctgctcttcc atcagggtgt tggtgaccgt ggtgaccgcc tgggggatgt cgtgcgggtc
     3421 ctgcaggacc ttgccgacgc gggtggcggt ggcgcggtgg ccgtcgtggg gggcttcctc
     3481 ggcagcgttg acggtgaccg tcgggagcgt cttggccgag gacggggtgg attgcgcctg
     3541 cgcgcccgcc gacaggacgg cgagcatgat cgcgctcagg ggtagcagcg ggcgggcggg
     3601 ggcgtgcgcg gactgcgccg cgccggaacg aacggcagct ttcatcgtga ctctctaggg
     3661 ttggatcggg aagtcggtgc tggctggctt cccgatcgac ggagtgacga tgaccgtttc
     3721 aggtggctgg ctgggcgaga cgttgagaat tatgtaaaaa acgagaatcg ttcgcaacaa
     3781 atttcagcga gtcgagatgg atcccggctc gtggtcgtgt tgttctcgct ccgccaagcg
     3841 gcttgcggcc cgcggtgcgc gtgccggctg cggctcagtt gcccgcctgc cccgcgctcc
     3901 acccgccccc gagcgccttg tacagattca ctgcagcggt gagtcgggcc aggcgcgtga
     3961 gggcgagttg gtcctgcgac gcgaacaggg tgcgctgggc gaggagtacc gtgagcaggc
     4021 cgtccgcgcc ttcgcggtaa cgcagttcgg ccaggcgcag gctgcgctcg gcttcggcga
     4081 ggatctcgcg ctcggcggcc tcctggttgg tgtcgcggac gccgttggcg agggcgtcct
     4141 cgacttcctt gagggcggcg aggatcgcgc ggcggtggga ttcgaccagc tcgcgctggc
     4201 gcgagcgctg gatgtcgacg tccgcgcgca ggcggccgcc gtcgaagatc ttctgcagca
     4261 cgctggccga gatcgacagc gtgcgtgtgg tgtcggccag cgccagcagc gcggcgctgc
     4321 ccacgcccgc accggccgac aggctgatcc cgggcagcag cgcggcgcgc gcggcggcga
     4381 tgttggcggc cgcggcgacg agccgggctt cgctggcggc gaggtcgggg cggcgcagca
     4441 gcagctcgga cggcaagccg gcgccgaccg cgggcacctt cagggccccc aggcgctcga
     4501 tgccgccagc gccgtcgggg gtgggtccgg cctgcgggtt gcggccaagc aggatcgcga
     4561 gcgcgctgcg cgtctggcgc agttgcacct cgagcggctc gatggccttg cgctggttga
     4621 gcaccgtggt gcgctgctga ctcagatcga gcgccgaggc cgcaccgttg tcgtagcgcg
     4681 cctgcaccac gcgcagcacg cgctcggcgg tggcgaggtt ccggcgcgcg atgtccaggc
     4741 gctcctgcag cgccaggacc tggaaccagg cggtggcgac gctggcgctg atcgacaggc
     4801 gggccgcgtc atagtcgaag cgggtggcca tgagaccggc gcggctggcg ccgacgccgg
     4861 cggcgatccg gccccagagg tcgatctcgt agctggcgcc gaggtcgatg ccggtcgagc
     4921 tggattcatt gctgtcgaca ttgcgtgtgc tgctcccgcc gctcacgtcg agtgacggca
     4981 atagcgacgc cccggcctgg cgcagcgcaa gctcggcctg gaccacgcgt tcggcctgga
     5041 tgcgcaggtc gggggtggtg gccagcgctt cggtgacgaa ggcgtcgagc cgggccgagc
     5101 cgaagtcctg ccaccaggtg tcatgcagcg gtgtggcggg ttctgccgcc cgggcgggtt
     5161 ccgcccactg cgccggcagc gcttgctcgg gacgcacgac gggctcggtg agcgcgcagc
     5221 cggcggtcag ggcggcgagc agggcaggga gcagacgggt gcgcaggggg aaaccggatg
     5281 gatttgtcat tcggaggcga gggcgacgac ggggtcgagt cgggcagcct tgcgcgcggg
     5341 caggtagccg aacaccagcc cggtgaggaa ggcacaactg aaggcgagca cgaccggcgt
     5401 cagcgagtac tgcaccgcgg tgccgaaggc ctcgatgatc gcggcggcgc cgagcccggc
     5461 cgcgaccccg atcagcccgc ccaccgccga caccaccagg gcctcgatga ggaactgctg
     5521 caggatgttg cgggtgcgtg caccggtggc catgcggatg ccgatctcgc gggtgcgctc
     5581 ggtcaccgac accagcatga tgttcatcac gccgatgccg cccaccagca gcgagatcgc
     5641 cgccaccgtg ccgagcagca cggtgagcgt gttctgcgtc tccgacacgg tatcgatcac
     5701 cgaggccatg ttgcggatct ggaagtcctc cacgccgtgg cgggcgagca gcagcgcgct
     5761 taccgcggcc tgggtgtcgt cgatctgccc gacgtcctcg accgccacgg tcacattgcg
     5821 caggaatcgc tggccggtga tgcgcaggct cgccgtggtg tagggcatga agacgatgtc
     5881 gtcctggtcc tgcccccagg gcgtggcgcc cttgggcgtc atcacgccca tgacctggaa
     5941 ggggatgttg ttgaccagga tgaactcgcc caccgcgtcg cgcccgggga acagggcgcg
     6001 cgccaccgtc tggccgagca cggcgaccgt ggcgtagcgc gcctcgtcct ccgcgctgaa
     6061 gaaggtgccg ctcgccggta gccagttgcg cgccacgacg tagtcggccg aggtgccgtt
     6121 gacgctgctg cggtggtcga tgttgccggc gcgcagggtg atggtggcgc cctgctcggg
     6181 caccgaggcg agcacgttgg gcagctcggc gatcgcctgc acgtcctcga tcaccagggt
     6241 ggcgggggcg tcgcgtccgc gcgtgttggg tgcgccgggg cgcacggtga gcaggttcgt
     6301 gcccatcgcg ctgacctggt cgatcacctt ctgcttggcg ccgtcgccga tcgccagcat
     6361 ggcgatcacc gaggcgacgc cgatgacgat gccgagcagg gtcaggacgg tgcggaagag
     6421 gttggcgcgc agcgcgcgca gcgcggtgcg ggtggcctcc accacgtccg acagcggcga
     6481 ggtgcgatcg atgtgggggg cgaagtcggg ctccggctcc tgcggcgggt gcggcccggg
     6541 gtcggcgacg atgcggccgt cgcggatctc gatgatgcgc tgcgcctgct cggcgacctc
     6601 gcgctcgtgg gtgatcagca cgatggtgtg gccggcggcg gagagctcgg cgagcagctt
     6661 catgacctcg gcgccgcttc ggctgtcgag cgcgccggtg ggctcgtcgg cgaagatgat
     6721 gcggccgccg ttcatcagcg cgcgcgcgat cgacacgcgc tgctgctggc cgccggagag
     6781 ctggccgggg cgatggtgga tgcgctcgcc gagcccgagg cgcgccagca gcgcctcggc
     6841 acgggcatgg cgttcctcgc gtggcatgcc ggaatacacc gccggcacct cgacattgtc
     6901 gcgcgcggag gcgccgccga gcaggttgta gctctggaag acgaagccga aggcctcgcg
     6961 gcgcaggcgc gcgagcgcgt cgcggtccag cgccgcgacg tccgtgccca tgaagcggta
     7021 ggtgccgccg gtggggcgat cgaggcagcc gaggatgttc atcagcgtgg atttgcccga
     7081 acccgaggtg cccatgatgg cgacgaactc gccagggtgg atggtgaggt cgatgccgtg
     7141 cagcacgcgg gtctcgacct cgccattgac gaagctgcgc gtgatgcccg agagctcgat
     7201 cagcggtgtt gcggtcgtgg gcggggcggc gggccgcgtg gccgctgcgc gcgcgctggc
     7261 ggacgaggtc gcgtcctcgt cgggaaggcg ttcgatcttc atagccgcgg cgtcatctgc
     7321 gggcgaccgg cgccgtttcc gaccaccagc ccggagacga cgcgttcgcc ctcggccagc
     7381 ccggacagca cctgtgcctg cacgcggttg gagacgccga gctcgacctt gcgttcttcc
     7441 agcgcgccgc gtgcatcggc gacccgcacc gtaccctggc gcggtccggc gccggaggtg
     7501 cccgacgctg cgccgcgccc ctggctcatc atgccgcgcc ccgagcccat gccattgcct
     7561 gcgggcgccc cggcgccggg cggggctgcg ggggcggggc cggcccctgc ctggggcggg
     7621 cgcgcctggc cctgggcgag tgcggccatc gggatcagca gcgcatcctt ggcctgcgcg
     7681 accacgaaga agacctgtgc ggtcatctgc gtcatcagcc ggccgtcggg attgaggacg
     7741 tcgaagagcg cgttgtagag caccacgttg ttggtcaccg tgggcgtggg ttcgatcttc
     7801 tcgagcttgc cctgccagcg cttgccgctg ccgcccaggg tggtgaagta ggcctccatg
     7861 ccgagcttca ggcggccgat gtcggcttcc gatacctggg tctgcacggt catcgtggac
     7921 aggtcggcga tgcgcatcac caccggtgcc tgctggttgg tgttgagcgt ctggccctgg
     7981 cgcgcagtga tcgacaccac ggtgccggcc atcggcgaca ggatgcgggc gtattgcagg
     8041 ttggcctcgt cggcgcgcag ggtcgattcg atctgctcga tctgcgcgcg cagcgcctcg
     8101 agctgagcct ccgccgacat cgccgaggct tcggcggtct gcaggctctc cttggtggtg
     8161 gcgtcctcgg ccatcagcgc gcgctggcgg cgctgctgga tctgcgcgag ggcgagctgg
     8221 gcctcgcggt ccttcagctg cgcacgcagg ttcttcaact gggcgcgtga ggcgtcgacg
     8281 cgggcacgat agaccgtggg gtcgatctcc gcgagcagct ggccggcctc gaccgtgtcg
     8341 ccgacctcga cgtggatctt gcgcaactga ccggagacct gcgcgcccac gtccacatag
     8401 tcgcgcggct gcagggtgcc ggtggcggtg acgacgtcct cgacgtcgcc gcgctgcacg
     8461 gtggtgaact ggtagttggt ggtggggtcg cgctcaccga tgaaggcgct gcgaccgtac
     8521 caggcgccgg cgagcagcag cggcaccagg atcagcaggg cgcggcgtcc ccagcgcggg
     8581 cgggcggttc ggttggaggg cttgtcttcg ttcattgggc tcagggcttg ccggcggacc
     8641 gccgggcgct gcatcgattc gtgggcaaga gcatagcggg ccgcgagacc tgcgcttgta
     8701 tcggattgaa gccgcgtcgt ttacggctgg agagaccgcc cgtgggacgc atcgttccgg
     8761 cgcgcgactc caaaacgttg accgtagtca agaaatagct tgtttagaca gagtctaatt
     8821 agcgcgtcgg aacatcctgc tccgggcagg gtgggaaacc aagaagcgac aattcgccga
     8881 actggcaagg agaagcatca tgatgaagtt gaaggcaacg agcgtggcgg tggcattggt
     8941 ggtggctggc agcgcctggg cggccgtggc ggtgcgggac gaaccgatca agccgatcga
     9001 gccggcgaag gtggagaacg ccgccaaggt cgagctcggc aagaagttgt ggttcgagcc
     9061 gcgcctgtcg atgtcgggca tcatctcctg caatacctgc cacaacctgt cgatgggggg
     9121 caccgacaac ctcaagacct cgatcggcca cggctggaaa gccggtccgg tgaactcgcc
     9181 gaccgtctat aactccagcc tggcgatcgc ccagttctgg gacggccgtg cggccgacct
     9241 caaggagcag gccggcggcc cgatccaggc cggcgtcgag atgaacatgc cgcacaccct
     9301 ggcggtcggc atcctgcagt cgatcccggg ctacgtcgaa gagttcgagt cggtgtacgg
     9361 caagaacaag atcgacatcg acatgatcac cgactcgatc gccgagttcg agaagaccct
     9421 ggtcacgccc gattcgcgct tcgacaagtg gctcaggggc gacgacaagg cgctgtccgc
     9481 gaaagagctc gccggctaca ccctgttcaa ggaaagcggc tgcgtggcct gccacaacgg
     9541 cccggcggcc ggcggcacgt cattccagcg catgggcgtg gtcgagccgt acaagagcag
     9601 ctcgcccgcc gaaggccgct cggcggtcac cggtgccgat gccgaccgct tcaacttcaa
     9661 ggtgccgacc ctgcgcaacg tcgcgctgac ctacccgtac ttccacgacg gcgaggcggc
     9721 gacgctcgag caggcggtgg ataccatggg gcgcctgcag ctcggccgca cctacagcaa
     9781 ggatgagatc ggacagatcg tcgccttcct gaagacgctg accggcgaac tgccgcagat
     9841 cgcgatgccg gtgctgccgc cgtcgagcaa cgcgacgccg cgtcccgatc cgttcaagta
     9901 atccgaaaag ccatccggct tgtcgcagga atgccggggc acggacgcag gttcgtgccc
     9961 cggtactagt tctcatggat tattgacacg atccgggacc cggtccagca ttcagacagg
    10021 gtccaatgca aatccgcaca gggtgcggat gcgcaaccag aaggtcgact gccatgatga
    10081 acacctacgc atcctccgac gccatcgcct gcctgcgcca ggccggcatt ccggtcacgc
    10141 accagcgcat cgagattgca cgcgccctgt tttcggcgcc ggtgcatctc tcggcggacc
    10201 agatctgggc gaaggtgctc gaggcctcgc ccgacgtctc gcgcgccacg gtgtacaaca
    10261 ccttgcggct gttcagcgag aagaagatga tccgcgagct gttcgtcgat ccccagcgcg
    10321 tggtgtacga ctccacgacc gcgccgcacc accacctcta caacatcgac agcggcgagg
    10381 tgcgcgacat ctcggacgac gagttgcgcg tgatcggcca gccccgcctg cccgcgggcg
    10441 tggtgctcga cgcaatcgac gtcatcgtcc gcgtgcgcaa ccgcgccgtc tgatcgcctg
    10501 ctcccgggag ccgggagcga gggggccgca atcgaaataa gggcgccagg gcgcggaatc
    10561 gctccgcatc ctggcgcctc ctttcattcc ttcctcgacg ttcagcgttc cggcacggtc
    10621 acaaagccca tcttctcgat gccggcgctg cgcgcctcgg ccatcacctc cgcgatcacc
    10681 tcgtagcgcg tgttgcggtc ggcgcgcagg tgaagctccg gctggggctt ttgcgccgcg
    10741 gcggcgctca gacgggcggg aagggccacc tcgtcgaccg cctcgttgtt ccagaacacg
    10801 cggccgtcgc cgtcgatcga cagtgccacc gtctccggct tctgttcggt cggcgcgctg
    10861 gaggcctgcg gcaggtcgat cttgaccgag tgggtcagca gcggcgcggt gatcatgaac
    10921 acgatcagca gcaccagcat gacgtcgatc agcggcacca tgttgatgtc gctctgcggg
    10981 gcgcgcgtct cgccctggct gaatcccccg aaggccatca cgccgctccg tgcgcgccgg
    11041 ccggcgtgcc cgtggcgact tcggccgcgc gtggcgcggc gccgggcact gcagcgatgc
    11101 gtgcgccggt ggcgaaccag gcgtgcaggt cgtgggcgaa ggcgtcgagt tcggagagct
    11161 cgacgcggtt ggcgcgcgtg atgccgttgt aggccagcac cgccgggatg gcgacgaaga
    11221 ggccgaaggc ggtcatgatc agcgcctcgc cgaccgggcc cgccaccttg tccagcgtcg
    11281 ccatgcccga aacgctgatg ttcaccagcg cgtggtagat gccccacacc gtgccgaaga
    11341 ggccgacgaa gggggcggtc gagccgatcg aggcgagcac ggtgaggccg gactcgagcg
    11401 ccgcggtcga cagcgcgatc gacttgcgca gcgcgcgggt gatgacctcg tcggtgtcga
    11461 gcttgccgcc gagggtgttg ctgtgggtgt gccggcgcac gtgctgggcg gcggcggcgc
    11521 cctgctgggc aagcgcctcg aagggcgact ccggggcgcg ctcggcgagc acgcgcagac
    11581 cggcgtcgag gtcgggggcg gcccagaagg cctctacggc gccgctgtgg ctgcgcgcac
    11641 gcagctgacg cagggagcgg ctgaggatca ggtaccagct ggtgatcgac atcaccagca
    11701 ggatcagcgc caccgcgcgg atgatgttgt cggattgcgc ccacaggtgg gcgaggccga
    11761 aggcctgcga actgtccatg ctcagtttcc ttgaagtttg aagacgatgg gcaccaggac
    11821 ccaggattcg atcgcgctgt cgccgcgccg tgcgggcacg aagcgccagc gcgccaccgc
    11881 ctcgcgcgcg gcgcggtcga ggcgctcgga gccggagctg gtgcgcagct cgacctgggc
    11941 gggctggccg tcgggcgaca ccagcacgcg cagcgtcacc tggccctcct cgcgcaggcg
    12001 gcgcgacagc gccgggtagg ccggatgcgg gttgttcaga taggcggcgt cgaagcgcgc
    12061 ggcggtgacc gtggcgggcg cggccgtcgc tgcgggtgct gcggcgggtg gtgccggcgc
    12121 gggcggcgga ggggctgcgg gttcttcggc cgcgctcagc gcggtgggcg actcggtgac
    12181 tggcgccggc ttcttcacca cgggcttggg cgtcggcttc ttcaccatcg gcggggttgg
    12241 cggcgtgggc tcgggcgggc gcgccaccgg ccgaggttca ggcgggcgcg gcggctccac
    12301 gacgggttcg ggaggcgccg gtaagggctc ggcgaccgcg ggcgctgcga tgagtgcgac
    12361 ctggatcggc atcagctcgg cgggcacggg gcgctcgccg gcgagcctgg cgagcatcgc
    12421 cagcccgccg aggtgggcgg cgacgatcag gccgaggagg gcgccgcgcc gcagcggcga
    12481 gctcccgcgc gctgccgcgg tcgggcgcgg agcgacttcc gctccggagg caggggcgcg
    12541 catgggctgc gcctcaggat ccgtggacag cgacggcgaa ggggaaggca acagcgacaa
    12601 tgcttgggca gctgggtgca tcggggcgga ggcgacgagc cgcctccgcg gaggcagcgt
    12661 tgggttggtc gggagcgatc gggcgggccg ggatcaggag gcgccggggt ccatctgcga
    12721 ctgcaggtag ttctgcaggc cgacgctgtc gaccagaccg agctgcgtct ccaggtagtc
    12781 gatgtgttcc tcggtgtctt ccaggatgtg ctcgaagagc tcgcgcgaca cgtaatcctt
    12841 gctggactcg cagtaggcga tcgcctcgat caggttggcg cgtccgccga gttcgagctt
    12901 gagatccccc tgcatgcatt cgggcacgtt ctcgccgatc agcagcttgt cgagattctg
    12961 caggttgggc agcccctcca ggaagagcac gcgctcgatc agcatgtccg cgtgcttcat
    13021 ctcctcgatc gactcgtcgt actcgtgctt gccgagccgc gtgaaacccc agttcttgta
    13081 catgcgcgca tgcaggaagt actggttgat cgcggtgagt tcgatggtga gctgcttgtt
    13141 gaggtactgg atgactttct tgtcgccttt catgttgcct ccagggatgc ggtgaatgcc
    13201 ggagcgatgc ctgcgaacag cgcggtcacg cccgggcggg gcgtggcggg tacggccgag
    13261 cgcaggcact cgtgcgcgca gcgggcgcag cgaccacact ctgcggtcac gccgagcgaa
    13321 tggcgcaggt cgcgcagggt ccgcgcgccc tgcgccgcgg cgtggtggat ctggcgttcg
    13381 gtgaccgcgt tgcatacgca cacgtacata gtctagcctc ctgacggggt tttgccccga
    13441 tatggcatcg cctggaaggt catttggtga ggatcagctt gccgttgcgc gtcgtgcgca
    13501 agatgtactc gctgccctcg tgctcgatcc gcaactcgcg ccggccgccg aggagctcct
    13561 tgctggtgag cagggggcgc cgctgtgcag tcgaatccgc atcgggccgg ctggccggca
    13621 tggcggcgga aacggggaat tcgcaaggtt gcatgatcga acgacgttaa aaatacgaat
    13681 gattcgcatt aaatcacgag tcgccgacgc atgcaatgcc ggcgcgcatc cgctgcccgc
    13741 tggcttcact ccaggagcat caggacgttc aggggctggt tggcggcaga ggcggcaggc
    13801 aggtaggcga gcgcgttcgg gtcggcgagc acgcggccga gcgcgtcggc cgcgtcggcc
    13861 gcctcgtggg gcggatgggc gcgcccggtg aagaccatcc gcgaccagta ggcgcggatc
    13921 tggctggggt tcttgccggt caggcgctga tagaagaggt cgcgttcggc gccggccggc
    13981 aggtcgagca ggcggacgcc acgcccgtcg ggcagggtgg tgcggcggcc gaggtagagc
    14041 tgggcggcgg tctcgcggtc gacttcggcg atgcctccgg cgtgggcgct gaccaggacg
    14101 acgccttgcg cgtgcgctcc ggcctgccag agggcgagca cgagcgtcag catcggcgca
    14161 agaccgagcc gtcggagcag gcgcgccgga tcagaacacc acatcgaccg acacgctcag
    14221 cagattgatg ctgcgatcgt cgagaccggg ggcgaagggg ttgccccgcg cgatgaagct
    14281 gccgtgcgcg ccgcgatcgg tttcgatgtg gctgaattcc gccttcaccg ccgaattcgg
    14341 cgtgaagtcc caacgcgtgc cgagggtgac gctgcgctgg gcctggttgc gcgagcggtt
    14401 gaagaccgcg accgctgcgg cgctttgtgg cgagtccgcc agcccggggt cggccagcgg
    14461 cgcgtcctgg cgctggcggg cgagaccgaa ataaggcatc catgcgccga agcgccgccc
    14521 gaccgcgaca taggctccgg tcgcgctgtt gacgaaggcg tccgcctgca ggcgcccgag
    14581 ctcggcgatc acccgccagc gcccgtcgtc ccactgcatg ccgagggagc tgaagctggc
    14641 gtgggcaccc ttcccgctca atcgcgcctg caggtcgggc gggatccgcc aggtgtcgac
    14701 gaggtcccgg tacgactgcg aggtccggat caccgccagc tccgcctccg cgtggctcgc
    14761 gctgaagctc aactcgtcgc gctccagcgt cacggtgagg ccgcgcagcc ggcgcagatt
    14821 cgcgcgtccg ccaccgatca cggggatgcg gctgctgcca agatacgggt gcagctcgat
    14881 gaaggatccg ccgatcgggc tgcgatgcag caggtcgatg ccatccatgt cggcgaaggg
    14941 gttgagcgcg tagacctctg ccggcgggcg gacccagggc tggctgtagt tgatgtcggc
    15001 ggtctcggac agcatgaaga aggggctgcg caggcgcccg gcacgcacgg tcagcgcggg
    15061 gctcagggcg tggctcagga aggccagggc aggccgcggc gtgtagtcgc cgcgctgggt
    15121 ctcgcgcgtc acgacctgca gcaccgctcc ggtgccgtca ccggactgcc agccgagctg
    15181 taccccgagc accgagtccg gcccggtgtc gacgcggttg ccgcccggcg cgtcgatccc
    15241 gacgcgggcg aactccaggt cgtccgcgcg gctgtagacc gccccggcgg tcgcgaagcc
    15301 cgacacctgc aggtcgggtg cggcgcccgt gccggggctg gacgccagcg cggcgatgcc
    15361 ggcgagcagc aacaaggtgc ggcgagggct catcggcgct ccggagcggg attgtgcaag
    15421 cggttgaagg tggcgtcgcg gtcgagcggc cgcgagaaca ggtagccctg tccgaagtcg
    15481 cagcccatcg cgcgcaggat ctcgagctgg gcctcatcct cgatgcactc ggcgacgacg
    15541 gtcatgccga ggtcgtgggc gagttcgatg ctgtgccgca cgatcgcgcg caggcgctgg
    15601 tcgtacggca gcgagcgcac gaaggagccg tcgagcttga cctggttgca cggcatcgtg
    15661 tgcaggtagc tcagcgtcga gtagccggtg ccgaagtcgt cgatgaggat gtcgagtccg
    15721 gcggcgcgca gggcatggag gtggtcgatc gccgggccgt ccggatgggc gagcacgctc
    15781 tccgtgacct cgaagcggat gcgttcgcgc gccacgccga cacggtcgat ggcggcgagc
    15841 agttcggcgg cgagatccgg gcgtgcgagc tggcgcgcgg acaggttcac gctgacgcgc
    15901 agcggcgcgt cggctggcag ccggcccgcc cattcgcgca ggtcggcgca cacgcgctcg
    15961 agcacgagcg tgccgatgtc gtggatcagg tcgagcgcct cggcgagcgg gatgaactcg
    16021 cccggcgaga gcaggccgag cgtcgggtgc ggccagcgca ccagggcctc gaagctggtc
    16081 gtacgcaggt tcgcgagctc gacgatgggc tggtaccaca cctgcagccc gccctgggtg
    16141 agcgccttgc gcagttcggc ctcgacctgc agcgcgcgca ccgcggcggc gtgcatctcg
    16201 gtgtggaaga attcgatcgg cccccgctcg ctgcgacggg ccttgtgcag tgcgttgtcg
    16261 gcatcgcgca gcagcgattc ggcgtcctcg cgctcgttgt cgctgatggc gacgcccacc
    16321 cggcagcgcg ggtgcagttg ctgaccgtcg tcgtcgggtg cgaaggccgg cagcgcgacc
    16381 agctgctcgc agatgcgcag cgcgtcgatc gagctctcca caccgttgag cagcaccgcg
    16441 aactggtcac cccccacccg cgcgcacacg tcgccatcgc gcatcagggc cgcgatgtgg
    16501 gcaccgatcc ggcgcagcag ttcgtcgccc acgaggtgcc cgtagctgtc gttgaccagg
    16561 ctgaagcgct ccaggttgat cgccaggatc gcgatctggg tgaggtcccc gcgccggcgc
    16621 tgggcaagcg cgcggttggc gtgctcgatg aacattgccc ggttgggcag gccggtgagc
    16681 ccgtcgtgca aggcatcgtg caccagctgc tgttcggcgc gcttgcgcag gtcgatgtcg
    16741 ccgagcgaac cagccatgcg gtaggcatgc aggttctcgt cgcgatcggc gactccgcgc
    16801 atcagcaccc agcggtaggc gccgtcgggc aggcggacgc gcagttccat gatgaaatgg
    16861 ttgctgtcgc ccttgaggtg ttcgaccagg cgctggcggt aggcgtcgcg ctcgtagggg
    16921 tggaggtagg cgagcatgtc ctccggccgg ttcgacaggg ttcccggctg gaccccgagg
    16981 atctggcaga agcgctcgga gtaatagacg tcgtcggcgt cgatgtccca gtcccacagg
    17041 ccgtcgttgg acccgcgcag ggccagggca tggcgctcct cgctcgcccg caggcgcacc
    17101 gccgtatcct gcagctcggc gatccggttc tgcagcgcct cggccatcag gttgaagtgg
    17161 ttggcgatca ccgccagctc gtccttgcca cgcgactcga cgcggtgcgc gaggaagccg
    17221 ttggcgatcg cacgactgcc ctccagcagc aggcgcaggt tgcgggtgag cagatagccg
    17281 agcgaggaga gcaggacgaa ggtgagcagg atctccgcga tcgcgatcgc caggccttgc
    17341 tgcatgatcg cctcgcgggc cgcggcgagt accgagaccg agactccgaa ctgcagcacg
    17401 ccgacctggt tgcgcggcag cagcagttgc cggcgcacat gcacgaggtc gtcggcgagc
    17461 agggtctcga agaaacggtg gcgttgttcg tccggctccg gcacctgctc cagcccgggc
    17521 atgccggcgc tcaccagcag atgaccgcgc gcgtcgcgga tgcgcacata gagcaggcct
    17581 tcgtcggcgt cggcaagaag ctcgccgagc acgtcctcga gcccgtcgag ccggccctgc
    17641 tcgccgtagg cggtggccat cgcgtgcagc atgctcgcgg tctgaccgat cgtcgtctcc
    17701 aggctcgccg acattgcgtt gttcatcagc cgcacgctgt tcgcgagcaa catgctcagc
    17761 aacaccacct gcaccaccgt actcgcgagc aacaggcgga tgcggatcga cgaggggcgg
    17821 agggcgaacg agctcggcga tgtttcgggc ggcacgcgaa cctcagcgcc gggcgagcct
    17881 gcgcaccgcg gacgaggctg cgatgctgcc gttggcagcg aagctctcca ggttggcttc
    17941 caggcggtcc gggtcgtaca gataattgac catcaatccc caagactggc gcaggccctt
    18001 gtccgagcgg tcggcgagcc agttcaggcg ctcgatgcgg gcaccgttgc cgaggtggaa
    18061 gcgcgccacc gtgtcgaccg ggcggtcgcc gcggcgtgcc tcgctcaggt agcgtgcgcc
    18121 cagccgcagc aggggctcgc gcaaggactc ggcgagcgcc tcgtcctcga cccacgccgg
    18181 gtcgccggcg agcaccgcgg cgaggcgcgt cgagtcgacg ccttcgatgc cgagcgtcgc
    18241 caggcgcttc cagtcggtgg cggtgaaggc ctcggcgaag gcctccgggt cgcgctgcgc
    18301 ccagcgcacc aggcccggca tcggcgacag cgtggcgaag gtcttcaggc gcggaaagtc
    18361 gcgcttgagg tcgtcgatca cccgcttgag caggaagttg ccgaagctca ccccgcgcag
    18421 gcccttttgc gtggcgctga tcgaatagaa gatcgcggtc gtcgcccggc tcgcatcggc
    18481 gagcggcgcc tcgatgtcga gcaggcgctg cacgtcgtcg gcgatctcct cgagcagtgc
    18541 gacctgaacg aagatcagcg gctcgagcgg catgcgcgga tggaagaagg cgtagcagcg
    18601 gcggtcggag tcgagccggt tcttgaggtc cttccaggag cggatctcgt gcaccgcctc
    18661 gtactcgacg agcttctcga gcagggcggc gggcgaactc caggtgatgc gctgcagttc
    18721 caggaagccg acgtcgaacc acgccgacag gcgcgtctcg agctcgcggt cgagcggctt
    18781 gagcagcgga tcgtcggcga tgaagcgcag caggtcggca cgcaggtcga cgaggaactt
    18841 gaccccctgc gggatcgcgt tgaactgggt gaggatgcgc agccggcgcg agcgcagcgc
    18901 ggcgcgcagc tcggcttccg cgtcccattg ccgcggcgtg ccgaccgcgg cctggtaggc
    18961 ggcgtgcgcc tgcgccaccc gcgccggatc ggggccgaac tcgtgggcga tcaggcgcag
    19021 gaaggcgtgg cggccgacgt cgtccaggcg cagataggtc tcggccaggc gggcggcacg
    19081 ctggcgcgtc gatacttcgc cgccgcggcc ctcggcgcac tcctgcaact gcgtgcgcag
    19141 gcgctcgagg tcgctctcat ccgggggctt gcccaggtgc gcggcgctcg cgacgagctc
    19201 gcgaacgcgg ttcagtcccc gggagaccag tcctgcaccg cccatgtcgt cctcctgtcc
    19261 gattcagttc ggatatcgtg cttacatgat agcccgcttt ggggagtcgc ttgtgcagcc
    19321 tgcgaatgga ctgtgtgcga tattcacgtc cctgccgcag ccccgcctac cttccggtcc
    19381 cgcgcgtgcg cgcggcggcg agcatcagca gcccggcgag caccatgggc acgcaaagcc
    19441 attgtgcggt ggacaggccg agcgacaagg tgccgaagat gcccggatcg ggcgtacgga
    19501 agaactccgc gacgaagcgc agcgcgccat agccgaccag gaacatcgcc gacaccgcgc
    19561 gcagcgggcg cgggctggcc gagtaagacc acagcagcag gaacagcagc aatccctcgc
    19621 cagcggcctg gtagagctgc gagggatggc gcggcagcgc atccacccag gggaagatca
    19681 tggcccacgg cagctccgtc tccgccggcc ggccccacag ttcgccgttg atgaagttgc
    19741 cgagccgccc ggccgccagc ccggtcggca ccagcggcgc gatgaagtcg gtgacctgcc
    19801 agaagccctt gccgtgccgg cgaccgtaca gccacatcgc caccagcacg cccaggaagc
    19861 cgccgtggaa gctcatcccg cccttccaca ccgcgaggat ctcgcccggg tgttgcagat
    19921 agtagccggg ctggaagaac aacacctcgc cgaggcgccc gcccacgatc accccgatca
    19981 caccgtacat caggaggtcg tcgatcccct gctcgtccca gcccagctcc ggacggcgcc
    20041 gcgcgtggag gcggccgagc acgacgaaga gcacgaaggc gaccaagtac atcagcccgt
    20101 accagcgcac cgaaagcggg ccgagggaga aggcgatcgg gtcgaattgg gggtggacga
    20161 gcatggagtg tgcgaggcgc gaggcgcgag gtgtgaggtg tgaggtgtga ggtgtgaggc
    20221 gccgagcgca gggctgccgg ccttcagtcg aactgcttgc ggaactccgc gaacgctccg
    20281 cgcagtgcct cgagttcctc gcgcagacgg cggacctcgt cctcgagttc cccgatgcgc
    20341 ccgcgcccgc cgcctgccgt gccgacggac tgcgcctcgg ccatctcctg caccgcggcg
    20401 tcgccgccga gcaggtgaac gtggcgggtc tccttggtgc cgggcgcacg cggcagctcg
    20461 gcgaccagcg gcgggtactt ctcggccagg tgctcgagca cggcatctac cgcggcgacg
    20521 tcgtcgaaac ggtgcaggcg ctcgcagcgc tggcgcagct cgcccgcggt ctgcgcgccg
    20581 cgcagcagca ggatcgccaa tacggcctgt tcggccggcg gcaacgagtg gcgcaggcgg
    20641 accaggtgct cgtacttggc cacgcgcgcg ctctgctggt cgcgcttgga caccaggcgg
    20701 cggacgatca ggcggtcgac cgcgtcctgc acctcggcct cggacaggct catcaccggc
    20761 tcgcgcgcgg tgagctggtt gcatccggtg gtgatggcgt tgatcgacag cggataggcg
    20821 tcgggggtga tgaaggactt ctcgatcagc acgccgagca cgcggatctc ggccgggtcg
    20881 aggtcgaagc cggcctcggt cgtgggggtc gtgtcctgca tggagcctcc tgcggtgtcg
    20941 gacttcggat gatacgccag ccggatcgtc cgccccaccg cggcgtcccc gcatgcaccg
    21001 cagcttgccg acgctcgccc cacgcgcgtt agaatcgaac cctttcccgc cgatttcccg
    21061 gagcgcccca tgccccagta ccgttcccgc acgtccaccg ccggccgcaa catggcgggc
    21121 gcccgcgccc tgtggcgcgc caccggcatg aaggacggcg acttcgaaaa gccgatcatc
    21181 gcgatcgcca acagcttcac ccagttcgtg cccggccacg tgcacctgaa ggatctcggt
    21241 cagctcgtcg cgcgcgagat cgaggccgcc ggcggcgtcg ccaaggaatt caacaccatc
    21301 gcggtcgatg acggcatcgc catgggccac ggcggcatgc tgtattcgct gccctcgcgc
    21361 gagctcatcg ccgacagcgt cgagtacatg tgcaacgcgc acacggcgga cgcgctggtg
    21421 tgcatctcga actgcgacaa gatcaccccg ggcatgctga tggccgcgct gcgcctgaac
    21481 atcccggcga tcttcgtctc cggcggtccg atggaggccg gcaaggtcaa gtgggaagcc
    21541 aaggtgatct cgctcgacct cgtggatgcg atggtcaagg cggccgacaa gtcgtgttcg
    21601 gacgaggaag tcgacgccat cgagcgctcg gcctgcccga cctgcggctc gtgctcgggc
    21661 atgttcaccg ccaactcgat gaactgcctc accgaggcgc tcggcctgtc gctgcccggc
    21721 aacggcacca cgctcgccac ccacgccgac cgcgagcgcc tgttcaagga agccggccgc
    21781 cgcatcgtcg atctcgcgcg tcgttactac gaaaaggacg acgcctcggt gctgccgcgc
    21841 tcgatcgcca gcttccaggc cttcgagaac gcgatgagcc tggacgtggc catgggcggc
    21901 tcgaccaaca ccgtgctgca cctgctcgcc gccgcgcgcg aggccggcgt ggacttcacg
    21961 atgaaggaca tcgaccgcgt cagccgccgc gtgccctgcc tgtgcaaggt cgcgcccgcg
    22021 atcgccgacg tgcacatcga ggacgtgcat cgtgccggcg gcatcatgtc catcctcggt
    22081 gaactcgacc gcgccggcct gctgcacacc gatgtgccga ccgtgcacag cgcgagcctg
    22141 ggcgaagccc tggacaagtg ggacatcaag cgcaccgaag acgaagcggt gcacaccttc
    22201 ttccgtgcag cgccgggcgg ggtgccgacc caggtcgcct tcagtcagga ccggcgctgg
    22261 aagtcgctcg acgccgaccg cgagcacggc atcatccgca acaaggaaca tgccttcacg
    22321 gccgacggag ggctggccgt gctctacggc aacatcgccg agaagggctg catcgtgaag
    22381 accgccgggg tggatgaatc catctggaag ttcaccggca aggccagggt gtacgagagc
    22441 caggaagacg cggtggaggg catcctcggc gagcaggtgc aggcgggcga cgtggtggtg
    22501 atccgctacg aaggcccgaa gggcggcccc ggcatgcagg agatgctcta tcccacctct
    22561 tacctgaaga gccgcggcct gggcgcgcag tgcgcgctgc tcaccgacgg acgcttctcg
    22621 ggcggcacct cgggcctgtc gatcggccat gcgtcgcccg aggcggcctg cggcggcgcg
    22681 atcgcgctgg tcgaggacgg cgacacgatc gagatcgaca tccccgcgcg ccgcatccat
    22741 cttgccatcg ccgacgccga gctcgcccgc cggcgtgccg cgatggaggc gaagggcaat
    22801 gcggcctgga agccggtgaa gcgcgaacgc gtggtctccg ccgcgctgca ggcctacgcc
    22861 gcgctcacca cctcggccga caccggcgcg gtgcgggacg tcacccaggt ccagcgctga
    22921 tcgaccccgg ccgattgacc cgtcatcggc cgctcactac catcgacgcc atggataccg
    22981 aactcgcaca gctcgaagcc cagctcgagc acctgatcag ccactacgag cgcctgaagg
    23041 ccgacaacgc cgccctgcgc ggtcgcgtcg tgcagctcga gggcgagaat cgccggctct
    23101 ccgacaaggt ccgcgtcgcc gcggaccgcc tcgcgggcct gctcgacaaa ctgccggagg
    23161 cttgaagcga tgtccgccga tcacctcgac atgatgctgc tgggcaagga gtaccgcatc
    23221 tcctgcacgc ccgaatcgcg cgacgaactg ctcgccaccg tcgccttcct cgacgacaag
    23281 cttcgtgaga tcggtgcccg caccggctcc tccggcgaaa agctggcggt catgaccgcg
    23341 ctcaacatcg cccacgagtt cctcctgcac cagcgcggca acgggtttga catgccgggc
    23401 atgaagcgta gaatcggtct catgaaagcg cgcctggacg gtgttctggg gcagcaggac
    23461 aagcttttct gaaggcagtt cgagcagtac cggtcaatcc cctgcagtgt tcgtcgaagt
    23521 cgtagattcc ttgaaccaat agtcgagaga ctcggtcgcc aaccatcgat gccgctggtg
    23581 tgaacgcccc tcgccgggtg atcctgaagc ggaagggacg cgacctccct gaacccaagg
    23641 ttcaggatgc ccggcggaga cggcacaggc ggggggcctt acaagagggc gcggtgagcg
    23701 atcaccgcgc cctcttcacg tccacgcgcc cgacacctcg ctccctgcgc ctcacgctga
    23761 tgctgcactg caaactctta acgcaatctt tacgcatggc cgcctagtat ccgcagcgtc
    23821 catacacagt ctcgcggcgt ttgccgtcct tgtcgcgatg caagcctatt tccccgtcct
    23881 cggtgtgctc ggcatgatgc tgatgctgtt cgggctggtg atgggctttc cgctcgcggt
    23941 gtccttcgcg ctcgaagacg gtgccaccga ggcctacgac ctcgccatcc tcgccacctt
    24001 cgcgaccggg ctgctcatgt gggccgcggg ccggcgggcg cggcgcgacc tgcacgtctc
    24061 cgacggcttc ctgctcgtgg ccgcgacctg gctgatcacg ccggtgttcg gggcgctgcc
    24121 gctgctgttc taccttccgg aactctcgtt caccgacgcc tacttcgagg ccgcctccgg
    24181 gctcaccacg accggtgcca cggtgcttac cgggctcgac acgatgccgg tgtcgatcaa
    24241 cctgtggcgc tgcttcatgc actgggtggg cggcatgggc gtgatcgtgc tgatggtcgc
    24301 ggtgctgccg ctgctgggca tcggcgggcg gcagatcttc cgcgccgaga cgcccgggcc
    24361 gatgaaggag tccagcctca ccccgcgcat cgccgagacc gccaagggcc tgtggggcgt
    24421 ttatgtagtg ctcaccgcag gctgcaccct cgcgctcttg caggccggca tggcgccgtg
    24481 ggatgccgtg atccatgcct tctcgatcat ggggctgggc ggcttctcca cccgggacgc
    24541 cagcctcggc gccttcgaca gcgtgccgat cgagaccgtc accatcgtgt tcgcgctgct
    24601 ctcgggcatc aactatgcca cccactacct cgccctggtg cggcgcacgc cgcgccctta
    24661 cggcgaggac cccgagctac gctggttctt cggcgtgctc ggggtcaccg tggtctttct
    24721 gaccgtgtat ctgctcgaat cgggcgccta tcgggatggc gcgctcgcgt tccgccacgt
    24781 ctccttccac gccgtctcgc tggcgacctc gctcggtctg gcgacctacg actacacggt
    24841 gtggccgatg ttcgcgcagg tgtggattct gttcctgagc agcttcgccg cctgctccgg
    24901 ctcgaccggt ggcggcatca agatgatgcg cgcgatcatc ctgtacaagc aggttcaccg
    24961 cgagatcgtg cgctcgctgc atccgagcgc ggtggtgccg ctgcgtctgg gcaggcagcc
    25021 ggtgtccgac aacgtgctgc acgcggtact cgcgttcagc ttcatgtaca tggtcagcat
    25081 cgtcagcctg acgctgatcc tcgccgcttc cgggctggac ctgatcaccg cgttctcggg
    25141 cgtggtggcc tgcatcaaca atgccgggcc agggctggcc gcgctggggc cggcgggcaa
    25201 ctaccagggc ttgggcgatt tccagacttg ggtgatgagc tttgcgatga ttctcggccg
    25261 cctggagatc ttcaccctgc tggtggtgct gacgccggcg ttctggcggc gctgagcggc
    25321 tcgggcacca gccggtagcc cacgccgggt tcggtgagga tgtggacggg caggcgcggg
    25381 tcgggttcga gcttgcggcg caaaccggcc atatagacgc gcaggtaatg tgcgtgctcg
    25441 acgtggttcg ggccccaggc gccggtcagc agctggcgct gcgtcaccac gcgacccgct
    25501 gcggtggcga gcaggccgag caggcggaac tcgatcgggg tcaggtggac cggcgcgccg
    25561 ccacgcgtca cccggcgctg ctcgaggtcg agttccacgt cgccgaaggc gatgcacgcc
    25621 gtggcctgcg gcggacgcag cgcgcggcgc agcagggcgc ggacgcgggc gagcaactcg
    25681 gcgacgccga agggcttggc gagatagtcg tcggcgccgg cgtcgagcgc cgcgaccttg
    25741 tcggtctcgt cgctgcgcgc cgacagcacc agcaccggca tgccgctcca gccgcgcagc
    25801 tcggccagca gcgccgtgcc gtcgccgtcg ggcaggccga ggtcgagcac cagcaggtcg
    25861 ggccggcggc tggcgagctc gatgcggccg cgctcgagcg cggcggcctc gcgcaccgtg
    25921 cagccctcgg attccagcgc cgtgcgcacg aaacggcgga tgccggcctc gtcctcgacc
    25981 agcagcacga ccggggaggg gggctgggcg ctcttcatcg tgggggcgct ccggcctcgt
    26041 cctcgggagg cggtcggtcc ggctggcgtg gcagcggcag ctgcagcacg aagcaggcgc
    26101 cgccgccggc gcggttgtgt gcgctcacgc tgccgccgtg tgccgcgacg atggcgtgca
    26161 cgatcgccag acccagcccg gtgccggcat cgccggtcgc cgtcgttgcg tcggccgcgc
    26221 cgcgcggatg gggggcgcgc acgaagcgct cgaagatcgc ctgctcgcgc cccggcggca
    26281 ggccgcaacc gtcgtcgcac agactgatgc ggatccagcc ggcgccgacc tgcgccgcca
    26341 gcacgatgtg ccctccctcc ggggtgtgcg cgatcgcgtt gtcgagcagg ttgcacacca
    26401 cccgctccat cagcaccgcg tcggcgtgca gcagtggcag ttcctcgggc agctcgagcg
    26461 cgaacacgtg cgcgcgcagg gcgtgggcgc gcgcctgcag cgcgctgccg agcagctcgt
    26521 ccaccgccag ccagtcgagc gcgaggcgcg gcgccccgga ttgcaggcgg gagaggtcga
    26581 gcaggtcgcg cgcgagcgca tgggtgcgca aggcctcggc gcggatcgcc tcggccagct
    26641 cggcctgcgc cggcggcagc ggcggtccgg ccagcgggag cgactcggcg agtccggcga
    26701 gtgcggtcag cggcgtgcgc aggtcgtgcg acagcgaggc gagcaaggtg ttgcgcaggc
    26761 gctcggtgtc ggcggcgagg cgcgcctgtg ccgcctcttc cgcacgcgcc tcggcagagt
    26821 cgcgttcgcg gcgcaggcgc gcggccagct ccgcggtggt cagtgccacc gcgagcagca
    26881 cggcgaaggt cagcaggtac tgcaggtccg agaccgccag cgtgaagtgc ggatgcacga
    26941 agaagaagtc gaacagcgcc accgccagga aggcggcgaa cacggccggt ccgcgaccga
    27001 gccggatcgc tgcgaacagc accgcgagcg ggaacagcat ggcgatgttg gcgagatcga
    27061 actgcccgag cagcggcgca ccgaccagcg ccaccgcacc cgccgccagc gcggcgagca
    27121 ggtaattgcg cagcggggcg cggtcgggaa ggcggtcgcg tggtgtcggc agggccatgc
    27181 cgcgatggta ccagcgtgcc catcccgccg cgtccgcgcc ggccccgccc gggaggattc
    27241 ccacggtccg cttgcactgc ccctcggctt tgaccgatag ttccgctcct tgcgcactgc
    27301 aacacgcggc gtgcctcgaa cccgaacgga aacgcaatga ccttcgacct ctggtggctc
    27361 gcctacccgg tgctcggcgc ggcggtgggc ttcttcgccg gcctgctcgg tgtgggcggc
    27421 ggcggcatca tggtgccgat gttgacgacc ttcttccttg cccagggctt cccgcagtcc
    27481 caggtggtgc acatggcgct cggcacctcg atggcggcca tcgtgctgac ctcggtgtcc
    27541 agcctgcgtg cgcaccacgc gcgtggcgcg gtgcgctggg acgtggtgcg cgcgatcacg
    27601 cccggcatcc tcgtcggcac cttcgccgcc actttcatcg ccgcgcgcgt gtccaccgtg
    27661 ccgctggcga tcttcttcgc cgtcttcatg gcctacgtgt cgatccagat gctgctcaac
    27721 atcaagccca agcccgcgcg cgagctgccc gggggactcg gcatgagcgc ggccggcctc
    27781 ggcatcggcg gtgtgtcggc gctggtcgcg atcggcggcg gctcgctgtc ggtgcccttc
    27841 atgacttggt gcaacgtcaa gatgcagaac gcgatcggta cctccgcggc gatcggcttg
    27901 ccgatcgcgc tcgccggcac ggtcggctac ctggtcaatg gctggggcgc acccgacatg
    27961 ccggcgctca ccttcggcta tgtgtacctg ccggcgctgg tgctggtctc cgtggtcagc
    28021 accttcttcg ccccgctcgg cgcgaagctg gcccaccgcc tgccggtggc gacgctgaag
    28081 aaggtgtttg ccgggatgct ggtcctgctg tgtgccaaga tgctgcacag catcttcgcc
    28141 tgaacgaact cgctggacga atctgccgga gacttccatg agctggacga tgtggcttgg
    28201 ctttcttgcc gccgcactgg tgatcgcggt ctcgccggga ccgggcgccg tgctgtcgat
    28261 gtcgaccggc ctgcgctacg gctacaccgc cgcgctgcgc gcgatcaccg gcctggagat
    28321 cgcgctgttg ctgcaactcg gcgtcgtggc gctcggcctc ggtgcggtgc tcgcgacctc
    28381 ggagacggcg ttcgcggtgg tgaaattcgc cggcgcggcc tacctgatct ggctcggcgt
    28441 gcagaagtgg cgctcgccgc ccgagccgat cgacggggac ggcgagggcg tgcgggtgca
    28501 gtcgcgccgc cagctctacg ctcagggcat cctggtgaac ctgaccaacc cgaaggcgat
    28561 cgtgttcatc gccgcgctgg tgccgcagtt catcgtgccc gaccggccgc agtggccgca
    28621 gttcgcgatc atcggcgcaa ccatggtcgg ggtcgacgtg atggtgatgt cctgctacgc
    28681 gctgctcgcc acgcgtttcc gccccctgct gcgcgacccg cgcctgctgc gcctgcagaa
    28741 ccgcctgttc ggcggcctct tcgtcggcgc cggggcgatg ctggcggcct cgagccgctg
    28801 agccgccccg ggtccgggcc ggcaatgggc gatggcgcga acggcggcgc cgtaatcgcg
    28861 gcttgcgtcg ctcccacaag aacgctccgc cgcccccgag ccccggcagg agcgacgcaa
    28921 gtcgcgatcc cccgcgcccc gagacccgcc gccgctccgt ggaaaccgat ccggttcagc
    28981 gctcgggtgc cgcgagtgcc ttctgcagcg tcgccaggtt ggcgcgcatc atgcgtacgt
    29041 agctgggcgc cgggccgtcg gccttcgaca gtgcgtcgga atacagcgtg ccgcccacga
    29101 ccgcgccgct ttcgctgcgg atgcgctcga tcaggcgagg atcggcgatg ttctcgatga
    29161 acaccgccgg caccttttcc gccgcgagct ggcggatcag gccggccacg cccttggcgg
    29221 tgggctcggc gttgttcgcc acgccgacgg gcgacaggaa gcggatgtcg taggcgcgcg
    29281 cgaagtaccc gaaggccgcg tgcgaactca ccaccttgcg gcgctcggcg ggcagcgccg
    29341 cgaaggcggc acggatctcc gcgtcgagcg cctgcaactc gccgtcgtag cgtgcggccg
    29401 cggcgcggta ggtggtcgcg ccgtccgggt cggcggtggc gagggcggcg gcgatgttgg
    29461 cgacgtagcg gcgcacgttc gccacgtcct gccaggcgtg cgggtcgacc gccttgccct
    29521 tgccatgatc gtggccatgg tcgtgcccgt cgtcgcgcct catctccagc gtcgagatcc
    29581 ccgcgctcgc caccaccacc gcccccttga atccgccggc gcgcgccagc cgcgtcatcc
    29641 agtcgtcgaa gccgaggccg ttgaccacca ccagcgcagc cttgccgatc tcgcgcgcgt
    29701 ccgagggacg cggctggaag gcgtgggcgt cctcgtcggg accgaccagg gtgcgcacct
    29761 tgatgcgctc gccgccgacc tgggcgacga ggtcgccgag gatgctgaag ctggtcgcca
    29821 cttcgagcgc ctgggcgtgg acgctgccgc ccaggagcag ggcggcgcag gccgcgaggg
    29881 tgcgcaggcg gcgcaagcgg taggggaatc tcatcgtcgt cgggtccggg ttgaagaaag
    29941 gaaagaaggt gtactcgagc cgcagggccc caccgcggat ttccgccgcg atcgctttcg
    30001 taggagcgac gcaagtcgcg actccggtcg cagggcggtc gctcgtgtcg gcgactgacc
    30061 aagcgctgcg atcgcgactt gcgtcgcgcc cacaagagcc atcacgtctc cgcgtcgctc
    30121 ctgccggagc agtcgagctg cccgcaggcg acatcgggcc tcacgactcg aggtggtgcc
    30181 gtaccggcag gcgtccggcg agcaggccac cgggggccgc gagcagcgac acgaaataga
    30241 ccacgccgca caccagcacg atcgccggcc cggcaggcac gtcggcatgg aaggacagca
    30301 gcagcccgct cgcgccgctg ccgaaggcga acagcaccgc gagcagcagc agcatcggca
    30361 ggccgttcgt ccacaggcgc gccgcggccg cgggcaggat catgatgcct accgccatca
    30421 gcgtgccgag cgcgtggaag ccgctcacca ggttcaccac caccagcatc aggaagccgt
    30481 agtgcgccac cggcgaccac cggctcaccc cgcgcaggaa ggcggggtcg aagcactcca
    30541 tgacgagcgg gcggaacagc agcgccaggc tcgccagggt cacgctggcg atgctcgcga
    30601 tcagcagcag cgaggcgtcg tcgagcccga gcacggagcc gaagaggacg tgcagcaggt
    30661 cgaggttctg cccgcgcagc gacacgatca tgacccccgc ggcgagcgag agcaggtaga
    30721 aggcggccag gctggcgtcc tccttgagca ccgaggcgcg cgtcaccagc cccgcggcga
    30781 acaccaccac caggccggcg acgatgccgc ccaccgtcat cgcgcccagt gacaggccga
    30841 aggcgaggta gccgagcgcg gcgccgggca ggatggcgtg gctcatcgcg tcgcccatca
    30901 ggctcatgcg gcgcagcagc aggaacacgc ccaccggcgt ggcgccgagc gcgagcgcca
    30961 ggcagccggc gagcgcgcgg cgcatgaagg cgaactcggt gaagggggag atgaggaggt
    31021 cgaacactgc gaaggcctgg tttgctggcg cgggttgcgg attcagtggc ggggaaggcc
    31081 gcggcgcgga cgcggcgcgg tgtgtgccgc ggggtgcgga tgggcgtggc cgtggccttc
    31141 gtcgtgcgca tgtccatggt catgcgcgtg cggatgtgcc gccgcatcgg gcgccaccgc
    31201 gactgtcaac tcctcgatgt ggcattcgtg cgcgtgctcg tcgaaggctt ccgacaggcg
    31261 gcgcgcgcgc gccaggcgct cgggcgtcag cacctcggcg gtggggccga aggccaccgg
    31321 ctcgcgcgac agcagcagtg cggtcggaaa gtggcggcgc acctgctcga ggtcgtgcac
    31381 cacggtgagg atggtgcggc cctcgtcgtg ccagcccagg atcagctcca cgagatcgtc
    31441 caccgtgcgg ctgtcgatgg ccgcgaaggg ctcgtcgagc aggatcagcg gcgcgtcctg
    31501 caacatcagg cgggcgaaac gcgcgcgctg cagttggccg cccgagagcg atccgatggg
    31561 gcgcgactcg aagcccgcca tgcccaccgc ctcgagcgcg gcgcgcacgc gtcggcgctg
    31621 ctcgcgcgag acgccgccga aggcgccgat ctcgtgccac aggcccatcg cgaccacgtc
    31681 gaacaccgac accgggaagc tgtggtcgat ctcgccgcgc tgtggcatgt aggccagtcc
    31741 ccccgcgggc agctcgggca gcaggatgcg cccgccgatc ggccgcagct cgccggcgag
    31801 gcccttgagc agggtggatt tgcctgcgcc gttggggccc accacggcga cgagcgcgcc
    31861 cgcgggaatc tcggccgaca ggtgatgcac cgccgggtgg cggtcgtagc ccagggtgag
    31921 gttgtcgagg cggatcatcg gcagcgcgag gcggttcatt gcaaggccca cagcacggtc
    31981 gcccatagca gcgccagcgc gccggcgacc agggcgaggc gcgcggccag gcccgcgccc
    32041 agcaggctac gtgccgggaa ggggcgcgtg tcgtcgtcgg cttggggatc ggtgccgtgc
    32101 aggacggggg gcgcgcgctt catcgttcct tcctctccac gccctcgcgg ccgcaatcgg
    32161 cgcacgcgcc atgcagcacc agctcggcgc gtgcgaccgc gaagccggcg ggcagggtcg
    32221 gcgcggcggc ggacacgtcc tccaggcaca cgatctggcc acagcgctcg cagtggaaat
    32281 gggcgtggcg gtggtcgccg tcgcggcgga tctcgaagcg ccaggcgcgt tcgccccccg
    32341 cgattcgctg cgcaataccc tgttccacca gccagtcgag cgcgcggtag agggtgacac
    32401 ggtcgtgggc gatgcccgcc ccgtcgagcg cggcgccgag ctcgtcgtgc gagagcggat
    32461 gggcgctgcg ctgcaaggcc tggatcaccg ccacccgcgt gcgcgtgacg cggccgccgc
    32521 gtgcggcgat gagctcgcgg gcagcgtcgg tggcggcagg gtgttcgtgc aggggcatat
    32581 cgaagcctgt tgcggcgggg cgcggggaaa cggctcttgt ggcagggtgg tcgcttttga
    32641 tgcaacattg tagcattaga cttgcgtgcc ccgcgctgca tggttttcag cactcgtcac
    32701 gcgccccgaa tcgcttgtct ccacttccca cgaccatgcc agcctgcttg cgtccgatcc
    32761 tcctctccag cgtcatcgtc ctggccctcg cgctccctgt cgccgcgccg gcacagcatg
    32821 cgcatggcgc acaccagcac ggcgtcgccg aactgaccgt tgtactggaa ggccgcatgt
    32881 tgcagctgga gctgatctcg ccgctcgaca acctcgtcgg cttcgagcat gcgccggcga
    32941 acgagacgca gcgcggtgcg ctcgccgatg cggagcgcct cctgctcgac agcgaggcgg
    33001 tgttcgcgct cccgtcggaa gctggctgcg tgatccgtga ggtcggcatc gaatcgccgt
    33061 ggcgggacag cgggcgcgaa cacggtcggg gccaaacacg cgaccacaac cggccggccc
    33121 gcggcgatca cgaggacatg gtggcaagct acggtttcga gtgcgcgcat cccgaggcgc
    33181 tgcgtcggct ggagttgcgc gtcttcaagc acttcccgcg cctgcagcgc gtgcgcgccg
    33241 agttcgccac cccgcgcggc caggggggcg gtgtgctcac ctcgggcgcc gccgcgctcg
    33301 cgctgtgagc gcggcagtgc gagcgcaccc gacgccggcg gtgcgcatct ccggcctgcg
    33361 ctaccgctgg ccgggcagcg cgcaggactg cctggcgatc gacgcgctgc agctcggcgc
    33421 cggtgagcgc gtcttcctgc gcgggccgag cggcagcggc aagaccacgc tgctgtcgct
    33481 ggtcggcggg gtgatcctgc cgcaggccgg cacggtcgag gtcggcgggc aggcgctcgg
    33541 cgcgctgtcg gcgggcgcgc gcgaccgctt ccgtgccgac cacgtcggct tcgtgttcca
    33601 gctcttcaac ctgctgccct ggctgtccgc gcgcgacaac atcctgctcg cctgccgctt
    33661 cgcgccgcag cggcgcgcgc gtatcgcggc cgcgggcagc acgccggctg ccgaggcggc
    33721 gcgcctggcg gcgcggctcg acctcggcgt cgagctgctc gagcgcccgg ccacgcagct
    33781 ctcggtcggc cagcagcagc gcgtggccgc ggcgcgcgcg ctgatcggcc gtcccggcct
    33841 ggtggtggcg gacgagccca cctccgcgct cgacgccgat cgtcagcaga ccttcatcga
    33901 cctgctgctc gacgaatgcg ccgcggtcgg cgccgcgctg ctcttcgtca gccacgacca
    33961 gcgcctggca gcgcacttcg accgcgtgct cgacctcggt gcgctcgtcc gcgccggcct
    34021 cgctgcggag gacgcgcgat gagccccgtc ttcatgctgt cgctgcgcag catcgccaac
    34081 cgcaggctgt cggccgcgct caccgtggtc gcggtggcgc tggcagtggc gctgctgctc
    34141 ggggtcgagc gcctgcgcaa cgatgcgcgc gcgggcttcg cgcagacgat ttccgccacc
    34201 gacctggtgg tgggcgcgcg cagcggcccg gtgcagctgc tgctgtactc ggtcttccac
    34261 atcggcgacg ccaccgccaa cctgtcgtgg gccagcatcg agcacgtcgc cgcgctgccc
    34321 caggtgaagt ggctggtacc gctcgcgctc ggcgactcgc accgcggtca ccgcgtggtc
    34381 ggcaccacca cggacttctt cgcgcaatac cgccatggcg aggggcgcag tctggtcttc
    34441 gcggccggcg gcccgttcgc cggcgcaacc gcgcgcgtcg aggacctctt ccagaccgtg
    34501 atcggcgccg aggtcgccgc ccgccatggt taccgcctcg gcgagcgcat cgtgctcagc
    34561 cacggcggcg gcgcggggga gggcggagcg cgcttcgccg agcacgccga caagcccttc
    34621 accgtggtcg gcatcctcgc gcccagcgcc acgccgctcg accgcgccgt gctggtgagc
    34681 ctggagggcc tggaggcgat tcatgtcgat tggcatggcg gcgcgcccat cccgggcctg
    34741 aagatcacgc ccgagcaggt gcgcaagttc gacctgcgcc ccaaatccgt cacggccgcg
    34801 ctcgtcgggc tgcacagccg cagcgcggtg ttccgcgtgc agcgccagat caacgcctac
    34861 gccggcgaac cgctcaccgc catcctgccc ggtgcgaccc tgcagcagct gtgggacctc
    34921 gtcggcatcg ccgagcgcgc gctgctcgcg gtctcggccc tggtggtcgt ggtcggcctg
    34981 acgggcatgg tggcggtggt gctggcaagc ctgggcgagc gccgccgcga gctcgccatc
    35041 ctgcgtgcgc tgggtgccag cccgcgcgag gtcttcgcgc tgctcgcgct tgagagcctg
    35101 ctgctcgcca ctgcgggcat cgcgctcggc ctcgggctgc tgtatggtgc gggcgccgcg
    35161 ctcgccccct ggctcgccgc gcagcatggt ctgcagccga gcctgggctg gcccgcggcg
    35221 ggcgaatggc gcctgctcgg cgcggtgctc gccgccagcc tggtcgccag cctgctgccg
    35281 gcgctgcgcg cctaccgcca gtcgctcgcc gacggcatga ccgtgcggac ctgaggcggg
    35341 atgcaccgcc tcgttcgcgg ggccgggagg gcccggatcc atcaccgcac gcctgcaacc
    35401 ggaggcagga aatgaacacg aatctgcacc tcgcccgctt ggtcccgcgc gcgctgcgcg
    35461 tcgtaccgct gcttgccctg ttggcgttcg cgccgctgcc ggctggcgcg caggccccgg
    35521 ccggcgagtc cgccggccag tacgcggtgg gcgaccgcct cgctgcgccc gcgccgggaa
    35581 agggcgagtt ccgcgagatc agctgggacg acctcatccc tgccgactgg aaccccgagg
    35641 gcttcctctc gggcctcgag ctcgacaagc tcgaggacaa cgacccgcgc gccgccgagg
    35701 cgctgcagcg catgcgcgag gagtgggacc gtgcgcccct ggtcgagcgc atggccggtg
    35761 cgcgcgtgcg catccccggc ttcgtcgtgc cgctcgagac cagcggcgag aagatccgcg
    35821 agtttctcct cgtcccgtat ttcggtgcct gcgtgcacgt cccgccgccg cccgccaacc
    35881 agctcatcca cgtcatcccc gacaagcccg tgccgggcgg ctggaacatg ctgccggtgt
    35941 gggtggacgg ggtcatggcg gtggggcgca tcgattccga catgggtacc gcgggctacc
    36001 agctgcgcgc ggtgaaggtg gaggagtacg tcgaggagaa gccgaagcgg cgatgaagcg
    36061 cctgcccgcg ccgggatcgc gactcgcgtc gctcccacag gcgctttcgg aagcgcggtg
    36121 acgtgccgtg ggagcgacgc aagtcgcgat tgcggcgccg cagtcctcgc cggccgcgca
    36181 tcgccgaccg gatcgcccga tcgcgactcg cgtcgctcct acaggcgcct tcggaagcgc
    36241 ggtgacgtgc cgtaggagcg acgcaagtcg cgattgcggc gccgcggcta gcgcccccgg
    36301 aacaccggtg cccgcttctc gctgaatgcg gcaaggccct cgcgatagtc cgcggtggcg
    36361 aggaagtcga aggcgcggcg cttctcctcc tcgtccagcg ccacgccggt ggtcagccgg
    36421 cgcacccatt gcttgtgcca gcgcgccacc agcggcgcgc ccgcgcggat gcgctcggcc
    36481 gacacggcgg cctcctcgaa tacgcgttcg tcgtccacca cgcgggtcag caggcccttc
    36541 tgcagcgcct cgcgcgcatc caggatgcgg ccctcgagca ggatttccag gaccaccgcc
    36601 gggccgacca gggcgagcag gccggccatc tcgcccggat acatcgagaa ccccaggcgg
    36661 ttgatcggcg cgccgaagcg tgccgactcg cccgcgatgc ggatgtcgca gcagccggcg
    36721 atctccagcc cgccgcccac gcaggcgccg cggatcgccg ccacggtggg cacggcgcag
    36781 tcgcggatcg cgttcagcgc gctcgccacc aggtcctcgt ggtagtgcag cgcatcgtcc
    36841 accgtcgcgc gcacgctgcg gaactcctcg atgtcgccgc cggcggcgaa ggcgttctcg
    36901 cccgcgccgc gcacgatcac gcagcgcacc gaatcgtcgg cggcgatgcg cgccatcgcc
    36961 gccgagagtc cgcgccacat cgccgcgttc acagcgttca gcttggtcgg gttgtgcagg
    37021 gtcacggtgg cgatgccctg gtcgatgtcg aggtcgattc ttgtgttcat cggttcgccg
    37081 ttttcgataa ataaaatttt tccatacgga tgtgtatgga gtcttatgga tgatatatca
    37141 tccgctcgcc tacgatgccg cgtcagacgg ccgccgaggc aggaggaaag gtggaggcaa
    37201 agcagttcgt gttgcttatc cttgggaggg agtgaaagtg tctagctcga tgtatgcgca
    37261 catccggcgc aacccgcggt tcgcggagct tgtcgccaag cgcacccgct tcgcgggcat
    37321 tctggcggcg atcgtcctga tcgtctttta cggcttcgtg ctgctcgtgg cgttcgcgcc
    37381 cgagctcatc ggcaagcgtc tgttcgaggg cagcaacctg accttcggca tcctcgccgg
    37441 cctgctgcag ttcatcttct tctgggtcct gaccctggtg tacgtccgcc gcgccaacgg
    37501 cgagttcgac gacatcaaca aggaactcgt gaaggccgcc tggaaggagg aaaaatgatg
    37561 aacgcccgta gcctttcccg tagcgccgcc ggcctgctcg cgctggccgc cagcccgctg
    37621 gtgctcgccg ccggtcccgc gatggacgcc gccgagaagc agccgctcaa cctccacgcc
    37681 atcggcatgt tcttcgtgtt cgtgctgatg accctgggca tcacctactg ggcggccagc
    37741 cgcaccaagt cgaccgccga cttctacacc gccggcggtg gcatcaccgg cttccagaac
    37801 ggcctcgcga tcgccggcga ctacatgtcc gccgccacgc tgctcggtct caccgcgatg
    37861 atgtacgcgc agggtgtgga tgcctacatc tacatgctct ccttcttcgt gggctggccg
    37921 atcatcctgt tcctgatggc cgagcgcctg cgcaacctgg gcaagttcac cttcgccgac
    37981 atcactgcct accgcctcga ccagggcaag gtgcgcacga tggccgcggt gagctcgctg
    38041 accgtggtgt gcttctacct ggtggcgcag atggtcggtg ccggccagct catcaagctg
    38101 ctgttcggtc tcgactacaa catcgcgctc gtcgtcgtcg gcgcgctgat gatggtgtac
    38161 gtcaccttcg gcggcatggt cgccaccacc tgggtgcaga tcatcaaggc ctgcatgctg
    38221 ctgatcggcg gtaccgcggt gggcgtgctc gcgttcagcc agttcggctt ctcgttcgac
    38281 gagctcacca accgtgcgat ggaagtgcac aagctgggcc agaagctgct caaccccggc
    38341 agcctgctcg ccgatccggt gaccgcgatc tcgctcggcc tcggcctgat gttcggcacc
    38401 gccggcctgc cgcacatcct gatgcgcttc ttcaccgtga ccgatgccaa ggaagcgcgc
    38461 aagtccgtgc tctacgcctc gggtatcgtc gcctacttct tcaacgtcat cgccatcatg
    38521 ggcctgtgcg cgatcatcat cgtcggccag aacccgcagt tcttcgaagg cggcgtgatc
    38581 ggcggcaagg tcatcggcgg cggcaacatg atcgccatgc acctggccaa cgcggtcggc
    38641 ggcaaccttc tgctcggctt cctgtcggcg gtggccttcg cgaccatcct cgcggtggtg
    38701 gccggcctgg cgctggccgg tgcctcggcg atctcgcacg acatctactc gcgcgtcatc
    38761 atgaagggca aggccgacga agcgaccgaa ctgcgggtgt cgaagttcgc caccatcggc
    38821 ctcggtgtgg tcgcggtgct gctcggcatg gccttcgaga agatgaacat cgcgttcatg
    38881 gtcgcgctcg ccttcggcgt ggccgcttcg gccaacttcc cggtgctgat cctgtcgatg
    38941 tactggaagg gcctcaccac caagggcgcc ctcgtgggcg gctactccgg cctggtcagc
    39001 gcggtgctgt tcgtgctgct gtccaagtcg gtgtgggtgg atgtgctggg caacgcttcg
    39061 gcgatcttcc cctacaccca gccggcgctg ttctcgatgc cgatcgcctt cttcatgacc
    39121 tggctgttct ccacgctcga caagagcaag gcggccgagg ccgagaagga agccttcgag
    39181 gaccagtacg tgcgcgccca gaccggtgtc ggcgccgcga ccgcctcgtc gcactgaccg
    39241 aagcgcgcgg ttccc
//
LOCUS       DAOQBS010000015        37398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_566_length_37398_cov_15.239295, whole genome shotgun sequence.
ACCESSION   DAOQBS010000015 DAOQBS010000000
VERSION     DAOQBS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 37398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37398
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_566_length_37398_cov_15.239295"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..487
                     /locus_tag="PKN25_03320"
     CDS             125..487
                     /locus_tag="PKN25_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89957.1"
                     /translation="MKIRPFVCSLALCLGLSAPNAGAEPAQGDEMERARCSGIIDLFV
                     AADPALEKDEQALAAAQDRAYVFLMWAHGYLSGRDGIDFARRPLNQDGITALVGEIYA
                     VCRNDEARLFLDAVKEIR"
     gene            487..888
                     /locus_tag="PKN25_03325"
     CDS             487..888
                     /locus_tag="PKN25_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89958.1"
                     /translation="MSEPAFNFRHGKRGDARRQLAALGLALVLLAGLWPQPAEAQSPR
                     ARERRVERSLATKDAIRHRQMKRAYVAGAVAQHRRERIRDRYEDRYYRDRYERYDRRD
                     DDDLSRVLIGVAVGAVATSIIMNSHQDGDAR"
     gene            885..1382
                     /locus_tag="PKN25_03330"
     CDS             885..1382
                     /locus_tag="PKN25_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89959.1"
                     /translation="MTIRSATRYLAGVVLCAGLALGAHAGTPEEDATLGLIREQFQDV
                     DLRRVIEVSASMKLDEQGAKRFWPIYRAYLAEIVALRDRQIDTLAEYARQLDAGWIDD
                     PTARTSLARSLALEQARVDARHHMIRKAGEVITPIQTLRLYQLELAMDASIGSRVLQQ
                     IPLAK"
     gene            1578..1778
                     /locus_tag="PKN25_03335"
     CDS             1578..1778
                     /locus_tag="PKN25_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89960.1"
                     /translation="MNTRLALFAGLIGLSLTGCGGHEVVRPTVDVSIGQQLIDLKRAR
                     EAGALSDREYERQRERLIDSVE"
     gene            1782..1991
                     /locus_tag="PKN25_03340"
     CDS             1782..1991
                     /locus_tag="PKN25_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89961.1"
                     /translation="MRTKAYPLLAVLGCLLLASCGGDTYVKVQGTTTISKGQELADLQ
                     RALDEGAIDRGDYDLLRKRILDRPN"
     gene            2136..3236
                     /locus_tag="PKN25_03345"
     CDS             2136..3236
                     /locus_tag="PKN25_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV89962.1"
                     /translation="MRIQKSVFALTTALLCLSLAACSERPAPVAAVEATRPAPILTVG
                     RRTADEALRLPGRVRAARRAELSFDVPGFVDSFSLEEGRRVKAGEVVARLDDRIYRSR
                     LDAARAEFERARNDLARYQRLWESEQAVARAEVDDRRARLEAARTSLAAAERDLADTA
                     IKAPFEGVITRRRIEPFTNVQAKQPIAELQDLRALEVVVNVPERLVRRMRPQQAAVAF
                     LEGERSVEGDRAAPGQVLRLELQSFAAEADALTQTYAVVLAVRSVPAGVNLLPGMAVS
                     LEPAADAGGAPAADAAITVPLAAVAADAQGRPGVWVVGDDERVARRPVRTGAIVGADI
                     VVESGLAPGERVVAAGVGALREGMAVRPLESR"
     gene            3252..6305
                     /locus_tag="PKN25_03350"
     CDS             3252..6305
                     /locus_tag="PKN25_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV89963.1"
                     /translation="MNIAEYTIRKSVVAWVATLLVLAGGWLAYERLGRFEDPEFVIRQ
                     AVVVTAYPGASPAQVAEEITDRIEGAVQQMQEVEEVTSVSRAGESLVKVEARLAFSGT
                     QAELDQVWDKLRGKIAEAARALPPGAAEPVVKGDFGDVFALFYAVTGEGYSPRQLYDY
                     VDLLRRELVLVPGVARVALLGERREAIYVEVSSARAAQLGVPLERIYASLRAQNLIAP
                     AGAVDAGPLRLQIQPADGVVSVAALGELVVAGDAASGLLRLRDVAEIRREYVDPPAVL
                     MRHDGQAAIGLGISNVLGGNVVEMGDAVKRRLAELEGQRPVGMELNVVSFQSDSVRAA
                     VAGFVDNLVAAVAIVVVVLLLFMGLRSGLIIGAVLVLTVAGTLIAMWMDGIAMQRISL
                     GALIIALGMLVDNAIVVTDGILVRMQKGEAVGPAAGAVVRGTVWPLLGGTVVGILAFS
                     AIGLSPTDMGEYAGSLFWVILYSMLLSWLFAITLTPLLCAAFLKVKVEAGAREGGLLR
                     AYRRLLQGVLARRVASGVVLLGLLAAGVLAFGHVPPGFMPDSARPQFVVDLALPQGTD
                     IRTTAAVVAEAEAKVSAAPGVSHVTSFIGQGGLRFMLTYSPEDPNPAYGQLLVDVERF
                     EDIAGLIERLQPELEAAFPQASVKVWKFMLGRGGGKKIEAAFRGPDPAVLRRLAEEAK
                     AIMAADPGAVAIQDDWRQAVPVVRPHFSVEAAQRAGVLPSDIAAALERNYVGQQIGVY
                     RDKERLIPILARAPADERGGVEDLGNAQVFSATAGKYVPVSQFVERVDTVWEDALIRR
                     ENRFPTIKAQCDPPAGQLSAPLFERLRPQIEAITLPPGYVLEWHGEHKASVEANAGLA
                     AAAPFGFAAMVVAVIVMFNGLRQTAVIWLTVPLAVVGVAIGLLLFQAPFEFMAILGFL
                     SLTGMLIKNAIVLVDQIDVEIREGKPRLLALLDASASRARPVFLGAVTTVLGVAPLLV
                     DPFFRSMAVTIMFGLIFATGLTLVVVPLLYASFFRIRGDEAEAGR"
     gene            6302..7735
                     /locus_tag="PKN25_03355"
     CDS             6302..7735
                     /locus_tag="PKN25_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNV89964.1"
                     /translation="MRRASLPGCPRSALLPALALLLAACQPAAFVRPDVPVPASWPRQ
                     AEVAQAEAAASLGVQPWREILPAPELHALIEEALAANADLRIAIERIELARAQYGVER
                     AALFPGVDAAASVARERMPGFDPRANRIGENAVVGLSMPAWEIDLWGRLAARSEAARR
                     EVLASAALADGVRTSLVAQVSSLYLDLLDLDAQHEITRRTLDGRRKALRLTRARFDEG
                     VSSILDVRQAESLLAGSEQALADQARRIAQTENALAILLGRNPGPIARSARLDELRTP
                     ARLEAGLPAELLQRRPDIRAAEQNLRAAGASVEAARKAFLPRITLTTLLGFASTDLGR
                     LFDSDRYAWSLQPTLGLPLFDGGRLQAGVEVAEAQQRILVEQYKATIRQAFREVADAL
                     VAFERLTEQRAATRRLVHANRERLRVSKARYLAGISSYFEVIDAERQLFDSELGLAQA
                     TRGAQQAVVQLYRALGGGAAESSASNT"
     gene            7937..8719
                     /locus_tag="PKN25_03360"
     CDS             7937..8719
                     /locus_tag="PKN25_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNV89965.1"
                     /translation="MAAYWDEAAMQRALVMPSILAIGDSWFWYPFPGGSLISYLGPIV
                     EKKEHTILAQGMNGAEAFDYVDGKYKDRITGALRRYGSSLSAVFISGGGNDFAGFNDM
                     RPLLALDCSGAQSAADCFRSGDGGLGQFLERMDDHYRRLIGLIYTHTSLDCLILMHSY
                     DYAIPDGRGVAGKAGWLKPALVDAGVPEALQRDCVRYLIDAFHAMLGRIAMGDPAHLR
                     IVDSRGTLAEEDWANELHPKGKGFRKIAKERWKPVLQGAGLA"
     gene            complement(9056..9146)
                     /locus_tag="PKN25_03365"
     tRNA            complement(9056..9146)
                     /locus_tag="PKN25_03365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9110..9112),aa:Ser,seq:gga)
     gene            complement(9241..9621)
                     /locus_tag="PKN25_03370"
     CDS             complement(9241..9621)
                     /locus_tag="PKN25_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNV89966.1"
                     /translation="MPPHTPESTSHFDLAALRSDMSESRAAVRVVLETFAPWLTDTRQ
                     QLRAAIESAEAEPLGRVAHRLRGALAQLRAAPAVALVKKLEAHCKANPEACLSRDHAL
                     LAELDAELDALAAEVAAQLEALRR"
     gene            complement(9636..10004)
                     /locus_tag="PKN25_03375"
     CDS             complement(9636..10004)
                     /locus_tag="PKN25_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV89967.1"
                     /translation="MNKLAFIIDDSAVNVAVARLLLRRLGWVVEDFDSAIPMLARLAE
                     VQPVFMLLDISMPDLGGEEACRRIRANAAWNGVRVIAYTAHAMETERQRYLAGGFDAV
                     ITKPITIGGLHAAVGHAPND"
     gene            complement(10001..12568)
                     /locus_tag="PKN25_03380"
     CDS             complement(10001..12568)
                     /locus_tag="PKN25_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV89968.1"
                     /translation="METPRAIRGMSLRAKLIAIFIAIKVVPLVLLAVFAWKAAHDLGR
                     LVTERAITMSDVMRDTQMRTGQTAIDDAIDALDDRSREAIETLTTATARSIAQFLHER
                     DGDIRAAASLPADAAAYRSFVDTRVRNIEEHGPYALDAEGERWVERDPQPRELALVRA
                     PLPDNSNSFHYRAPDTHMRGTPRPLFLEITFIDRDGHERIKALSETGRQVLDPGLRDI
                     TRRENTFVRAETYWPALKALRPGEIHVSRVIGAQVRTHWIGDYTPTRAQALGKPFAPE
                     DSGYAGLENPVGKRFRGLIRWAAPVLRQDRIIGYVTLALDHAHLMAFTDTLRPTPERF
                     APIADPASGNYAFIWDDEGHNISHARDYFIHGYDPATGERSVPWLDRELYEAWRSSGR
                     SWPTFSAALTPYQDQRLTREPHPESTASGLVALDCRYLDFSPQCQGWHDMTEHGGSGS
                     FTILFSGLRKLTTVATIPYYTGQYGRTPRGFGYVTIGANVDDFHRAATESGQRIGKMI
                     GEADALTQHERDTLVGDIADQLRGTTTRLAASTLAMVLAVILIAIWMAGLLSRRITHV
                     VDGIHRFEQGDLDQRLAAAGGDELAELARAFNRMADTVQRSIRSLEDARHTAEQANRM
                     KSEFLANMSHELRTPLNGVIGFAELLSMELEDPEQRECATTIHDSARHLLAVVNDVLD
                     LAKVEANRLTLVAQTIDLVPLVASVAALHKVNAEAKGVDLRLDLPAHPCIVDADPVRL
                     RQIIENLLSNAVKFTREGHILVGLAAAADEISIRIADTGCGIARDEQERVFAPFYQAA
                     SFLNRGHGGTGLGLTLSRRLAHRMGGTIDLDSVPGKGSTFTLRLPRHFTAPAGGPPS"
     gene            complement(12596..13210)
                     /locus_tag="PKN25_03385"
     CDS             complement(12596..13210)
                     /locus_tag="PKN25_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV89969.1"
                     /translation="MAGKSDSNSEFISTAQAARQLGLSLGAVQQMVESGTLAGWKTAG
                     GHRRIRQDSVDALLAHARSPSAPMRSNRERLRVMVVEDDRLLQNIYRETFLTWSLPLD
                     VHLMEHGLDALVEVGREPPDLLIADLRIPGIDGFEMIRRLRDNPLSSDIAIVVVSALG
                     PKEIAAAGGLPEDITVYRKPIPFHELHGYVQAMISMRRRARHPG"
     gene            13421..13807
                     /locus_tag="PKN25_03390"
     CDS             13421..13807
                     /locus_tag="PKN25_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HNV89970.1"
                     /translation="MNTSKNVLGGALLACSYSPLTGFYRTGCCETGQDDLGRHVVCVK
                     VDAAFLEFSRRAGNDLSTPRPEYRFAGLKPGDRWCLCALRWKEALEAGVAPPVILEAT
                     HEAALRVIDLETLKAHAYGAAHGPSA"
     gene            13807..15240
                     /locus_tag="PKN25_03395"
     CDS             13807..15240
                     /locus_tag="PKN25_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV89971.1"
                     /translation="MASARAPLRERLAGLLAVLEAGLVERRQVLRASLLAALAGEHSL
                     LIGPPGTAKSALARRIHLAFRDARYFERLLTRFTVPEELFGPLSIRALEEDRYERHVA
                     GFLPDAAVAFIDEVFKANSAILNALLTLLNEREFDNGAGRLRCPLISVVGATNQVPED
                     EVAEAFFDRFLLRLTVAPVSAEGFRWLLGEDRPGSPEVLAPPSGLQLDEADRAALAQA
                     AAACALPAAVLDRLGELRAWLAGRGVYVSDRRWVKLAHLLRVAAASEGRAAASEWDLG
                     LAPCCVAADEPTRLAVADWLAARLGIREVWSPPRITRVVEAFEAQLATEREARDLDYD
                     ERGRLRFSAIDPVATQAGELAGSIGDAKGGAAALRMSAARMRRYGSLHIGARTAQLDG
                     VLARLAAYRGELGAQRATLEAWADQALWADPAFVACARSGLDAVDAVLAGLEARARSA
                     RAGFEALPRLEQAGAAPAPVEHDPLPE"
     gene            15251..16996
                     /locus_tag="PKN25_03400"
     CDS             15251..16996
                     /locus_tag="PKN25_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV89972.1"
                     /translation="MATGDAGSGVAPAVAQQSASAAADSAPAPLPPDRARAAEAPPVP
                     RLFEALEAVLAPLDRLPRPLWLGGMTHSGGELAGRLAALEAWRAALLAGGLPGSCAAW
                     PEAEVQEAVREVFRGLGLPAYCADQPALVDTVLQGLLFHLDLIVDYRDRGDTEAAAQA
                     RALDSFAADWAERCGEIDELVGAFGDLGDLLDNARWDALRGLLRSTDWREVLRIRALI
                     EGLPELARILRALGRACPTDEDAESSRALHAVVEHTEIQRSVSHRVRVPDLPGETRGV
                     QRSGRIARMLPAEATLLGHPRLRLVWHARRAERTLLAYEDDDHLQEDCLRPAPVLRPS
                     QRPAPARRLEQGPMLVCVDTSGSMQGGAEAVAKAVVLEAVRCAHARRRACRVYAFGGP
                     DEVVEMELGVDVDGVGRLARFLGQGFGGGTDICAPLERALARLDEAGWQLADLLIASD
                     GEFGATPALAARVEAARRERGLRVQGILIGDRETIGLLELADDIHWVRDWRRYGGGTD
                     KPGGADADKRRGDGAGANAPTLAAGGGSPVHSSHLTADYFPGALRTPENRAATVTPEA
                     AAAAIRAGRHRGDRL"
     gene            17148..17993
                     /locus_tag="PKN25_03405"
     CDS             17148..17993
                     /locus_tag="PKN25_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNV89973.1"
                     /translation="MSFATDLIAELERATAGCPIPRVRALHLPPPEAAASKNGEFCAL
                     ELDDGALGLSYVLLGGILPRLAASDDPHTIVGMDALQLAREFAAPAAGAGGDAEIRRT
                     LGFAAANALSRTVMERIGFAPPRATDSIGGIDPQAGDHVGMVGLFTPLLKQVTAAGAQ
                     LTVIELNPDYAGHYDGYRVTLDAAALEDCNKVLSTSTILLNHTVDDMLAHCRNARRIV
                     LIGPSAGCLPDPLFARGVTVVGGSWITDRAGFIDALRRGESWSGFAYKFALGAADWPG
                     LPENR"
     gene            18059..18997
                     /locus_tag="PKN25_03410"
     CDS             18059..18997
                     /locus_tag="PKN25_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV89974.1"
                     /translation="MHLTPRLVFFLVLPPLLWAGNAVVGRLAIGSMDPLWLNAVRWML
                     AFVLLLPLGWRALGTPAARAQIRARWAHLAVLGLIGVGAYNALQYMALRTSTPLNVTL
                     IASSAPVWMMLIGAVFYRVIPRPVQVFGALLSLVGVALVLSRGDPAAIARIEFVEGDL
                     LMLLAMLGWTGYSWLLARPPAHMAGEARPKWNWAEFLVAQCVFGVLWAVAAAAAGDVV
                     FPSGPTQWSWGLVAAIVYIAVGPSIIAYRSWGVAVAEAGPATAAIFYNLTPLFAAVFS
                     AAVIGEWPRPYHGIAFLLIVGGILVSTRAAARRQAA"
     gene            complement(19048..>19380)
                     /locus_tag="PKN25_03415"
     CDS             complement(19048..>19380)
                     /locus_tag="PKN25_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89975.1"
                     /translation="YAGNILGDETIEVGSRIPQELIDDLERDPRGNLRLKFRMSKDGT
                     LFGWDIQRRPGYDPNLRDPQGRDIHFPAVHSFAGGDFREAEIVNGKVVRKGWYIERRT
                     GRKVETDY"
     assembly_gap    19381..19480
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19529..25087)
                     /locus_tag="PKN25_03420"
     CDS             complement(19529..25087)
                     /locus_tag="PKN25_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNV89976.1"
                     /translation="MSCAPDSAQDGPSAPEPPAAGQCVSNNAGNPCGSASGPASQGST
                     TGQDVGAGNPIDVLSGNKYQQEVDLPALPGILGLEIVRHYNSRRARPGDIGALGSGWR
                     LSYETRLNIRDDRLEILQADGARVIFARRPGEPQRCASRDPSRGEILLRHRPGGGDEY
                     LWRWPDGRSLLFDSAGHLVQILVASGEFVSLRHDAAGRLLQVTDPQGRSLQVHYHAPG
                     SGIDGITHIDSPLGRFSYAQERVALPARTDAGTQPGRSVHAPQRPGSAGPSRPLQRGA
                     PAGKAAAPAVTAARLLRVDYPGAPAAQTGGAAAREYHYEDPRHPVALTGISVLARQPA
                     GPPVPERIASYAYDHAGRGIRSVRGALPAGGESGPEDVRLDYPERGRSVLTNSLGQRT
                     TYLGAVIAGQRRLLEARGPGCARCGPTDVRYRYTPDGRLSAITTLDAEGRPRLSTQHR
                     FDARGRLAETLGADLSGARPRLLDRTRYEYPDTPATTLADTDRAAQRTEPAATTPPRA
                     IIRPSVVPGREHRIELRYNATGQPIELRESGFSPIDAEGRPDPVPIERRVTWRYATIN
                     GRSVLAELDGPLPDGADASPLDSDLVRLHWDERGSFIRALEGPGGQRSEIESDPATGL
                     PRRVRDAEGHETHLRHDSAGQLIRWRSRSAGEAADRIHAAEYDALGQLVELRSGEDEE
                     RLHPRWRRAFDAAGRLQWHADALGILRTWAYDHESRVVETGLRSASRLLRRRWRYDEH
                     GRLAAIDDDTGFTRSLRHDVAGRLVGLLDSHGRELLPPARHPDGGSAPPAPPAHPRIL
                     KDDFGRPVLERSPDAGARWRSFDAAGRLVAMGDALGHRARYTWDPRGRILAQEVTDGR
                     NGSTETTRWRYDGSRLLEVDHPNGRERYEYDQRGLRSARIVSLKRDGGELVVVTRYEY
                     DAEGHLVATTLPDGSRLRYERNGQGQVVALKRQPVATPWLRALAREQVIASAFQRDLF
                     GLRGFRAGNGMRTLHERTRTGTLLRVVHLRADERGAPVRNASALLPIAPGQPLATRIE
                     RLFGISAAHAAQANTDADVTQPVEAADGQALEGALPRLQETVASADTILLDYQYVWDP
                     EGNLLHARRREPDGPLPSTRRSQAYDLRNQLVASVEWRDDGDVLAETAVWRYAYDRHQ
                     RRVLAQEGAVSQQELAGHTRPTHFAPGSHRALSSSPRAANVPARAAASRPHEAATPGH
                     HDASGQPERFGARSLRWDALGRLIEVRAGERSIARYAYDHRGLRIARTRFDPAMVAPT
                     TTHTVYDDARQPLAELDADGRLIRQYLWLADLPLAVLDTPARPDSATDSARRILADLG
                     RIAQSWLAPQAGLAWLHTNHLGAPELATDADGEPLWRARHAPFGAATVTTSPRRPDFT
                     LDLRLPGQVFDAETGLHYNRRRYYAPTLGEYLTPDPLGTPDGPNPYAYAAFNPLRNVD
                     PDGLVLFAFDGTGNSDDLNDPAMAGSGFSNVVYFFDAYTATKRYVSGVGTVHHDIDYG
                     DIRPEDHATGHLLWWLTPGDPAHVNDMGGNYSGPARIGRMSQYLDDEAELFSDDRVMD
                     IDIVGFSRGAAQAREFANRIVAKTVRHEGQDYYRYTNRRGDSACQAVDFRFMGLFDTV
                     LSTNFSGEAYRLGIPEVFAHVAQAVALNEHRSDSITEFAYRNPKPHRMHWGGFPLESI
                     GASSAAPGRIRIEKGFVGAHADIGGGYPDVEQGLSLVALNWMVAQARDAGVEMESVDP
                     VPILNVVIHDQSNVIEIGNPQHSVVPRPTGNGDLPRIDYVFPEDRAVNGAVAGDRQRR
                     MAFDNGSLTHADTLRYITWLPRDATRRGDGSALDPRRLGDVTGTVDMASYLAWLGQPE
                     NGYTTTNGGF"
     gene            complement(25345..26364)
                     /locus_tag="PKN25_03425"
     CDS             complement(25345..26364)
                     /locus_tag="PKN25_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNV89977.1"
                     /translation="MMNPAHPLPPARRPLPRLLPRPWRHRQRGAGAAEFVIVAPALLA
                     LTLAMLQTGLAFHARSIVNYASFEAARAGSIANAGLASMRLAFARAMTPYYGGGRNLA
                     ELADAFAAAQLDLAAALRIEIVSPTRESFDDYHSLAAARRLQTTARVIPSAQPGVLGC
                     PADRANCNADPASNRSGQTLADANLLKLRVTWGLPPAKQVPLAGRFFTWALRMLDPDD
                     DDAFRRGLVEAGRIPMVSQVTLRMQSDAIENEAMRPRTAGSATPPAADSTPLPGCTPT
                     APLCTPEPATADTAPEAADPTGEDGTPAGDGSGADGEPGPGTDSGDETEDAEDAEDAE
                     EEPEC"
     gene            complement(26361..26687)
                     /locus_tag="PKN25_03430"
     CDS             complement(26361..26687)
                     /locus_tag="PKN25_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNV89978.1"
                     /translation="MRVLVARSFLQRARGLLGRTHLAADEALLLRPCSSVHTFGMCFA
                     IDVVFIDPQGVVLAVREALGPWRVARCRGAAAVLEMAAGAARRAGLVPGTHFRLASTL
                     SVVVRA"
     gene            complement(26690..27595)
                     /locus_tag="PKN25_03435"
     CDS             complement(26690..27595)
                     /locus_tag="PKN25_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV89979.1"
                     /translation="MSSAHLLVLDERTVIAAVALLAGLGSAGIVAALARSVSAVRPED
                     RTWLDTPPRFLRLLWWPTRWIAHLVAPLLPARLQARLAVRLRLAGLDYTLSPAQLLAH
                     RVAVGLCGLAAGLGCAHAWALPPALPALAGATAGTLIAWSWLDDRIRCRRRLMLKQLP
                     FVLDLITLCVEAGLNLTGALQQAAAKGPAGPLGEELHRVLRDVRAGKSRADALRGFAD
                     RIGEPAIANLVSTVIQAENMGMSLGPMLRAQGEQRRAERFARAEKAAMEAPVKMLLPL
                     IACIFPCTFIVLGFPIAVKFMTMGL"
     gene            complement(27592..28461)
                     /locus_tag="PKN25_03440"
     CDS             complement(27592..28461)
                     /locus_tag="PKN25_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV89980.1"
                     /translation="MNALASSPAVLLATLAFGLASALLAWSAMRIAGNALARYRERFT
                     AEAHLQLEELFLFMDPARLFVLNLLAVTVGGIGSWLASGEVLIALASAGALALLPRLA
                     FGLLRRRRLDLIEQQLPDALQVIAGGLRAGVSMTVALQQLVREGRPPIAQEFDLTLRE
                     HRLGIPLDEALDHLAARVRMPSLTLVIAAMRIANETGGSLAEALERAALTVRSQLAME
                     GKIGALTAQGKLQAVVVGMLPLALLLVLDRMEPEAMGFLWHSPPGWATLAVIATLEVL
                     GVLLIRRIVAIDV"
     gene            complement(28458..29882)
                     /locus_tag="PKN25_03445"
     CDS             complement(28458..29882)
                     /locus_tag="PKN25_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNV89981.1"
                     /translation="MFFRTARKPPEDSSVALAAATDKPAAASAPISEPPVRPADEPHP
                     PAAEANADFTWRKRIHERLLDTIDLRRRDLNRMSDDELRAETTALVREIIAAEAALPG
                     GLDREQLCSEVLDEAIGLGPLETLLADESVSEIMVNRFDQIFVERGGRIAPHPTTFTS
                     DRAVLGVIERIVAPLGRRIDESSPMVDARLRDGSRVNAIIPPLALKGPTLTIRKFARR
                     ALEVADLVRMGSLSHEMAAFLRTCVEQRRNVVVSGGTGSGKTTFLNLLSNFIPDGERI
                     ITIEDAAELRLRHSHLVSLEARPSNLEGRGAISIRDLVRNALRMRPDRIVVGECRGGE
                     ALDMLQAMNTGHEGSMTTLHANSPRDALARLETLVLMAGMDLPLAAIREQIASAVDII
                     VQQTRFACGARRLTSITELTGMEGGRIQLQELFRFERRSADSPTADPSGGHFTGCDAV
                     PGFYDELRRQGVALDLHLFDRKRS"
     gene            complement(29895..31163)
                     /locus_tag="PKN25_03450"
     CDS             complement(29895..31163)
                     /locus_tag="PKN25_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HNV89982.1"
                     /translation="MFAGETRVLPQRDAQRLAVGDAKVLSAAVLDDREILLIANGPGD
                     TMLQVWTRSGRSQRIKITVRQTDTARIARDLQGFLHDVPRLRTRTIGDNVVIEGEGLS
                     DAERDKVTELAKRFPQLIDFTSRVGLDRMFAFDVRFVEISRSGLRDLGIDWTTQGKPL
                     LGIGVIGDLYHDNRTGATVTRTLGSGELSSEVEIEANRVSPFAANVALVGSFFGRLNL
                     LAQKGDAVILASPRLSARNRGEASFLAGGEIPVPVASATGTPSVTFKEYGIRLNILEP
                     VADAAGTIRARIRTEVSSLDRSVAAQGVPGLLSRRTETEFNLRNGETLVLSGVLQREQ
                     QSDENALPHLGELPVIGRLFRSTRFNSRESELVVFVTPWLLEPGEGLLDPRAQLLERR
                     AEQALAPAPEPSPALREPADPAWNALYGGN"
     gene            complement(31321..32181)
                     /gene="cpaB"
                     /locus_tag="PKN25_03455"
     CDS             complement(31321..32181)
                     /gene="cpaB"
                     /locus_tag="PKN25_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HNV89983.1"
                     /translation="MLKRVPRNLIILAVASLFGLGAAWLAARYLEERATEIELRQQGQ
                     MGHAVVAKTDLAVGTPLTRDLVAVREVPVEWLHSSAITPEQFERAAGAVLAQPARGGE
                     PLVWSQLEERRPATLSAQLAPGRRATTIPVDETSSLSGLLRPGDRIDLLATVQRDHHH
                     LTLPLLQGVRVLATGSRTRPGEDDGAGDLLGGSFGTLTLDLSEEEATRLVAARALARL
                     TAVLRHPRDTRTAPLQRTDALALLGLAPPAREAGVPVIYGGIENPALVPTPSPLTRPT
                     PRAEPAHDPD"
     gene            complement(32189..32368)
                     /locus_tag="PKN25_03460"
     CDS             complement(32189..32368)
                     /locus_tag="PKN25_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89984.1"
                     /translation="MKRIRRLRGQAMHEYLVILAIVLGVFGLPAFDGKPLLVFFAEAV
                     ATGFARFLASISLPL"
     gene            complement(32365..33147)
                     /locus_tag="PKN25_03465"
     CDS             complement(32365..33147)
                     /locus_tag="PKN25_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89985.1"
                     /translation="MPAHPAPRLPTSRHTTPALHPRPRPDGRGRGARAGLSIAFALLV
                     GSHAEAALPEPLPPLAGARITPVLDNARVHGVPTRIRRFEARTATASVRAFYEGAVEG
                     TPLSSRIDGWEVLSWKHGAALRTLRLRPCRTAPAACTEGTLSESDLSRDPAPSSPAPG
                     RPADSLLSTDLETNDAGRHARILVWHDESSPSWAAARLVRELSAQGLALERRAPVSRP
                     GLEGVGLWFGARGREAVATIVRHGSGTTVTLQMIDHGARSGR"
     gene            complement(33150..33920)
                     /locus_tag="PKN25_03470"
     CDS             complement(33150..33920)
                     /locus_tag="PKN25_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89986.1"
                     /translation="MKPCHPLLPAARAAQGGQSTTEFLVLALVLVPLFLSLPLLGKYL
                     DLAHATEQAARYVAFETSIVGPARQPPTAAALAAQVRERIFSTSGAPILSGAGNASAT
                     EHPANPLWIDHRGEHLLADPASRIGVDLAAHANAAPASALLAGPHGLDLPAGSEHTGT
                     IHVSPRDVPGLPPFDTLGLSIRRHQVLLGHNWSAAGSTDYARRIEDAGPLAYPIRPLA
                     LIGNTVGRLLPPLVLDKAIEVGRVHAEILPCDRLEQGC"
     gene            complement(33917..35341)
                     /locus_tag="PKN25_03475"
     CDS             complement(33917..35341)
                     /locus_tag="PKN25_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HNV89987.1"
                     /translation="MAATPRPGVAQRPQRADRQHGQALVLGLFLVFATLAVLLLMFNT
                     GRVVDEKMRLTNAADATAWSVATLEARALNYDAYTNRAIVANEVAIAQAISLISWMHY
                     FETAVENAPNLNQVAASWLYKPGEYPRLLQLLAGVNGTSYLDVGSGGSLRAIVEGLDI
                     GLGTIATAHDAVANALSLSQRVMHASLDTGIAQAGFANDFVRRIDPEMRASINPISHG
                     FRNLTRTMAKDSAQGDARQRIADVTLRSRDAFTRERVWSIRGLNLPPLQRKVELKRRG
                     GTELIGYDEWRALDTLEHQGQRMKRWRWRWRRTPLAWGGAGFTGDEGADARRGDHGDT
                     YTSNPITTLNRAEPAIVDAGAAHFGGLPETRELRDLSPAAEHRSGVTVHVAKARERLR
                     GSGGIGSVQPQGNLRAFEATAPGGHLAAMARAEVFFERPAARSDNKTERASLYSPYWQ
                     ARLAAVTPTDRAWAAGRQDGMALP"
     gene            complement(35371..35640)
                     /locus_tag="PKN25_03480"
     CDS             complement(35371..35640)
                     /locus_tag="PKN25_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89988.1"
                     /translation="MAAGRRIQWQRGQGMTEYIIIVALIAVAAIAVYQYFGQTVRNQT
                     AAIATEIAGKDGGTAKSAAQTAAGKAATEANTARTLDTYTGNAGR"
     gene            complement(35804..>37398)
                     /gene="ettA"
                     /locus_tag="PKN25_03485"
     CDS             complement(35804..>37398)
                     /gene="ettA"
                     /locus_tag="PKN25_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNV89989.1"
                     /translation="ISLSFFPGAKIGLLGLNGSGKSTVLRIMAGVDKEYDGEVQWLAG
                     QRIGYLPQEPELDPAKTVKEEVEAALGEIMEARQKLEEVYAAYAEPDADFDKLAEEQA
                     KYENILSTAGSDVETQMEIAADALRLPPWDAVIGKLSGGEKRRVALCKLLLSKPDMLL
                     LDEPTNHLDAESVEWLEQFLTRFPGTVVAVTHDRYFLDNAAEWILELDRGHGIPWKGN
                     YSSWLEQKGDRLAQEAKQEAAHQKAMKTELEWARSNPKARQAKSKARLARYEEMASVE
                     YQRRNETQEIFIPPGERLGDKVIEFHGVSKAFGDKLLMDNVSFAIPPGAIVGIIGPNG
                     AGKSTLFKMIEGRDKPDSGTVEIGATVKIAAVDQTREGLANDKTVFDAISDGADVLTV
                     GRFEMPSRAYIGRFNFKGGDQQKIVGNLSGGERGRLHLAKTLIQGGNVLLLDEPSNDL
                     DVETLRALEDALLEFAGCALVISHDRWFLDRICTHILAAEGDSQWFFFEGNYQEYEED
                     KKKRLGEEGAKPKRIRYKPIAR"
BASE COUNT         5006 a        12371 c        14145 g         5776 t
ORIGIN      
        1 ggtcggccgc cgagaaggtg ctggagaacg ccgccgagcg cctggcgacg gacctcgatc
       61 gaccctgagc cctcggccgc gcatgcgccg tcctgattgc cgtctccctc gaaggaaacc
      121 tgatatgaaa attcgtccgt ttgtctgcag cctggcgctg tgcctgggtc tttccgcgcc
      181 gaacgcgggg gccgaacccg cacagggcga cgagatggag cgtgcccgct gcagtggcat
      241 catcgatctg ttcgtagcgg ccgacccagc gctcgagaag gacgaacagg cgctggccgc
      301 cgcccaggat cgtgcctacg tcttcctgat gtgggcccat ggctacctga gcggccgcga
      361 cggcatcgac ttcgcccgcc ggccgctgaa ccaggacggc atcaccgcgc tggtgggcga
      421 gatctacgcg gtgtgcagga acgacgaggc gcgtctgttc ctcgatgcgg tgaaggagat
      481 ccgctgatga gcgaaccggc cttcaacttt cgtcacggca agcgcggcga cgcgcgccgg
      541 caactcgccg cgctgggcct cgccctggtc ctgctcgcgg gcctgtggcc gcaaccggcc
      601 gaggcgcaat cgccacgcgc acgcgagcgg cgggtggagc gttcgctggc gaccaaggat
      661 gcgatccggc atcggcagat gaaacgggcc tatgtcgccg gcgcggtcgc gcagcatcgg
      721 cgcgagcgga tccgggaccg ttacgaggac cgctattacc gcgaccgtta cgagcgctac
      781 gaccgtcgcg acgatgacga tctcagccgc gtgttgatcg gggtcgcagt cggcgcggtc
      841 gccacgagca tcatcatgaa cagtcaccag gatggggacg cacgatgacg atccgatccg
      901 caacccgtta ccttgccggc gtcgtccttt gcgccgggct ggccttgggt gcgcacgccg
      961 gaacgcccga ggaggacgcg accctcggtc tgatccgcga gcagttccag gatgtggacc
     1021 tgcgccgcgt gatcgaggtc agcgccagca tgaagctcga cgagcagggt gcgaagcgct
     1081 tctggccgat ctatcgcgcc tacctggccg agatcgtcgc cttgcgtgac cgtcagatcg
     1141 acacgcttgc cgagtacgcc aggcagctcg acgcgggctg gatcgacgac cccacggcgc
     1201 gcacctcgct ggcacgctcg ctcgcgctgg agcaggcccg ggtggacgcc cgccaccaca
     1261 tgatccggaa ggcgggtgag gtgatcacgc ccatccagac gctgcggctg tatcagcttg
     1321 aactggcgat ggatgcgtcg atcggcagtc gtgtgctcca gcagatcccc ctggcgaagt
     1381 aggcagtggc cggccagccg aagcgatgtt cgcagccgag tgcctgcagg cgggcgcctg
     1441 ttgcgtcggc ctggcccatt ttgggacgaa cgcggacatg gggcatgcgg gccggattaa
     1501 gctgcgcaaa tcggtttcgc ccgtcccgca tggccggttt cggtcgtgcc tgtcgccacg
     1561 ctcatgggat cctttcaatg aataccaggc ttgccctctt cgccgggctg atcggcttgt
     1621 cgctgacggg ttgcggtggc cacgaagtcg tacggcctac ggtggacgtc tccatcggcc
     1681 agcagctcat cgacctcaag cgtgcgcgcg aggcgggcgc cctctcggac cgcgagtacg
     1741 agcgccagcg cgaacgcctg atcgacagcg tggagtgaac catgcgcacg aaagcttatc
     1801 ccttgctggc cgtcctcggt tgcctgctgt tggcgagctg cggcggggac acctatgtga
     1861 aggtgcaggg caccacgacg atctcgaagg ggcaggagct ggccgacctg cagcgcgcgc
     1921 tcgacgaggg tgcgatcgat cgcggcgact acgatctgct gcgcaagcgg atactggacc
     1981 gacccaactg acgatcggtc ccgcgtcgtg cgtgccccgc atgcacgcgt cgtgtaaacg
     2041 ggccttgcgc gatgagcacg tctggtggct gcgccgcgcc ggcagtgcgc acgcgacccg
     2101 cgccccgacc gcccgcatcc gaccagaaga aattcatgcg cattcaaaaa tcagttttcg
     2161 cgctgactac cgcgctgctt tgcctgtcgt tggccgcctg cagcgagcgg cccgcgcccg
     2221 ttgcggcggt ggaggcgacg cgtccggcgc cgattctcac cgtcggacgg cgcacggcgg
     2281 acgaggcctt gcgcctgccg gggcgggtgc gtgcagccag gcgcgccgag ttgtcgttcg
     2341 acgtgcccgg cttcgtcgac tccttcagcc tggaggaggg gcggcgggtg aaggctgggg
     2401 aggtggtcgc gcgccttgac gaccgcatct accgctcgcg tctggacgcc gcgcgtgcgg
     2461 agttcgagcg tgcgcggaac gatctcgcgc gctatcagcg cctgtgggaa agcgagcagg
     2521 cggtcgcccg cgccgaggtc gacgaccgcc gcgcccgcct cgaagccgcg cgcaccagcc
     2581 tcgccgccgc cgagcgcgat ctcgccgaca ctgcgatcaa ggcgcctttc gagggcgtga
     2641 tcacccgccg ccgcatcgag cccttcacca atgtgcaggc caagcagccg atcgccgagc
     2701 tgcaggacct gcgcgcgctc gaggtcgtgg tgaatgtgcc cgagcgtctg gtgcgccgca
     2761 tgcggccgca gcaagctgcg gtcgcatttc tcgagggcga gcgcagcgtg gagggcgacc
     2821 gcgccgcgcc ggggcaggtg ctgaggctcg agctgcagtc cttcgccgcc gaggccgacg
     2881 cgctgacgca gacctatgcg gtggtgctcg cggtgcgctc ggtgcctgcc ggcgtcaacc
     2941 tgctgccggg gatggcggtg agcctggaac cggcggcgga cgccggcggg gcgcccgcgg
     3001 cggacgccgc catcaccgtc ccgctcgccg ccgtggccgc cgacgcgcag ggccggccgg
     3061 gcgtgtgggt cgtcggcgac gacgagcggg tcgcgcgccg cccggtgcgt accggcgcca
     3121 tcgtcggcgc cgacatcgtc gtggagtccg ggctcgcgcc cggcgagcgc gtcgtcgctg
     3181 cgggcgtcgg cgcgctgcgc gaaggcatgg cggtccgccc gctcgagtcc cgctgagccc
     3241 cgggtccgcg catgaacatc gccgaataca cgatccgcaa gtccgtcgtc gcctgggtgg
     3301 ccacgctgct ggtgctggcg ggcggctggc tggcctacga gcgcctcggc cgcttcgagg
     3361 atccggagtt cgtcatccgc caggcggtgg tcgtcaccgc ctacccgggc gcctcgccgg
     3421 cgcaggtggc cgaggagatc accgaccgca tcgagggcgc ggtgcagcag atgcaggagg
     3481 tggaggaggt cacctcggtg tcgcgcgccg gcgagtcgct ggtgaaggtc gaggccaggc
     3541 tcgccttctc gggcactcag gccgagctcg accaggtgtg ggacaagctg cgcggcaaga
     3601 tcgccgaggc cgcgcgcgcg ctgccgccgg gtgcggccga gccggtggtg aagggcgact
     3661 tcggcgacgt cttcgcgctc ttctacgcgg tcaccggcga gggctacagc ccgcgccagc
     3721 tctatgacta cgtcgacctg ctgcgccgcg agctggtgct ggtgccgggc gtggcgcggg
     3781 tggcgctgct gggcgagcgc agggaggcga tctacgtcga ggtctcgagc gcgcgcgccg
     3841 cgcagctcgg cgtgccgctc gagcgcatct acgcctcgct gcgcgcgcag aacctgatcg
     3901 cgcccgccgg tgcggtcgat gccggcccgc tgcgcctgca gatccagccg gcggacggcg
     3961 tggtctcggt ggcggcgctc ggcgagctgg tggtggcggg cgacgccgcg agcggcctgc
     4021 tgcgtctgcg cgatgtcgcc gagatccgcc gcgagtacgt cgacccgccc gcggtgctga
     4081 tgcgccacga cggccaggcg gcgatcggac tgggcatctc caacgtgctc ggcggcaacg
     4141 tggtggagat gggcgacgcg gtcaagcgcc gcctcgccga gctcgaaggc cagcgtccgg
     4201 tgggcatgga gctgaacgtg gtctccttcc agtccgattc ggtgcgcgcc gcggtggcgg
     4261 gcttcgtgga caacctggtg gcggcagtgg cgatcgtggt cgtggtgctg ctgctcttca
     4321 tgggcctgcg cagcgggctg atcatcggcg cggtgctggt gctgaccgtg gccggcacgc
     4381 tgatcgcgat gtggatggac ggcatcgcca tgcagcgcat ctcgctcggc gcgctgatca
     4441 tcgcgctcgg catgctggtg gacaacgcga tcgtggtcac cgacggcatc ctggtgcgga
     4501 tgcagaaggg cgaggcggtg ggcccggctg cgggtgccgt ggtgcgtggg acggtgtggc
     4561 cgctgctcgg cggcaccgtg gtcggcatcc tcgccttcag cgcgatcggc ctgtcgccca
     4621 ccgacatggg ggagtacgcc ggctcgctgt tctgggtgat cctgtactcg atgctgctgt
     4681 cgtggctgtt cgcgatcacg ctgacgccgc tgctgtgcgc cgccttcctg aaggtgaagg
     4741 tcgaggcggg tgcgcgggag ggcggcctgc tgcgcgccta tcgccgcctg ctgcagggcg
     4801 tgctggcccg gcgcgtggcg agcggcgtgg tgctgctggg gttgctcgcc gccggcgtgc
     4861 tcgccttcgg tcacgtgcca ccgggattca tgcccgattc ggcgcggccg cagttcgtcg
     4921 tcgacctggc gctcccgcag ggtaccgaca tccgaaccac tgccgccgtg gtcgccgagg
     4981 ccgaggcgaa ggtgagcgcg gcgccgggcg tgagccatgt gacgagcttc atcggccagg
     5041 gcgggctgcg cttcatgctc acctacagcc cggaagaccc caacccggcc tatggccagc
     5101 tcttggtcga tgtcgagcgc ttcgaggaca tcgccggcct catcgagcgc ctgcagcccg
     5161 agctcgaggc cgccttcccg caggccagcg tcaaggtgtg gaagttcatg ctcggccgcg
     5221 gcggcggcaa gaagatcgag gccgccttcc gcggccccga cccggcggtg ctgcgccggc
     5281 tggcggaaga ggccaaggcg atcatggccg ccgaccccgg ggcggtggcg atccaggacg
     5341 actggcgcca ggcggtgcca gtggtgcgcc cgcacttttc ggtcgaggcc gcgcagcgcg
     5401 ccggcgtgct gccctcggac atcgccgccg cgctggagcg caactacgtc ggccagcaga
     5461 tcggcgtgta tcgcgacaag gagcgtctga tccccatcct ggcgcgcgcg ccggcggacg
     5521 agcgcggcgg cgtcgaggat ctcggcaacg cccaggtctt cagtgcgacc gccggcaagt
     5581 acgtgccggt gagccagttc gtcgagcgcg tcgacacggt gtgggaggac gcgctgatcc
     5641 ggcgcgagaa ccgcttcccg accatcaagg cgcagtgcga cccgcccgca gggcagctgt
     5701 ccgcgccgct gttcgagcgc ctgcgcccgc agatcgaggc catcacgctg ccgccgggct
     5761 atgtcctgga gtggcacggc gagcacaagg cctcggtcga ggccaacgcc ggcctggccg
     5821 cggcggcgcc cttcggcttc gcggcgatgg tggtggcggt gatcgtgatg ttcaatggcc
     5881 tgcgccagac cgcggtgatc tggctcaccg tgccgctggc ggtggtgggg gtggcgatcg
     5941 ggctgctgct gttccaggcg cccttcgagt tcatggccat cctcggcttc ctgagcctca
     6001 ccggcatgct gatcaagaac gcgatcgtgc tggtggacca gatcgacgtc gagatccggg
     6061 agggcaagcc gcgcctgctc gcgctgctcg acgcgtcggc cagccgggca cgcccggtct
     6121 tcctcggcgc ggtcacgacg gtgctggggg tggcgccgct gctggtcgat cccttcttcc
     6181 gcagcatggc ggtgaccatc atgttcggcc tcatcttcgc caccggactc accctggtgg
     6241 tggtgccgct gctgtacgca agcttcttcc gcatccgcgg cgacgaggcg gaggcggggc
     6301 gatgagacgc gcgtcgctgc ccgggtgccc gcggtccgcc ctgctgcccg cgctcgcact
     6361 gctgctggcg gcatgccagc cagccgcctt cgtgcggccg gacgtgcctg tgcccgcaag
     6421 ctggccgcgg caggccgagg tggcgcaggc ggaggccgcc gcgagcctgg gcgtgcagcc
     6481 gtggagggag atcctgcccg cgcccgaact gcacgcgctg atcgaggagg cgctcgccgc
     6541 caacgccgac ctgcgcatcg cgatcgagcg catcgagctc gcccgcgccc agtacggcgt
     6601 cgagcgtgcg gcgctgtttc cgggcgtcga tgccgccgca tccgtggcac gcgagcgcat
     6661 gcccggcttc gatccacgcg cgaaccgcat cggggagaac gccgtggtcg ggctgtcgat
     6721 gccggcctgg gagatcgacc tgtggggccg cctcgccgcc cgcagcgagg ccgcgcgtcg
     6781 ggaggtgctc gccagcgccg cgctcgccga cggtgtgcgc accagcctgg tcgcgcaggt
     6841 ctcctcgctc tacctggacc tgctcgatct cgacgcccag cacgagatca cccgccgcac
     6901 gctcgacggt cggcgcaagg cgctgcggct gacgcgcgcg cgcttcgacg agggggtgtc
     6961 gtccatcctc gacgtgcgcc aggccgaatc cctgctggct ggatccgagc aggcgctcgc
     7021 cgaccaggcg cgccgcatcg cgcagacgga gaacgcgctc gccatcctgc tcggccgcaa
     7081 ccccggcccg atcgcgcgca gcgcccggct cgacgagctg cgtacgcccg cccggctcga
     7141 agccggcctg cctgcggagc tgctgcagcg ccgcccggat attcgcgccg ccgagcagaa
     7201 cctgcgcgcc gccggcgcca gcgtggaggc ggcccgcaag gccttcctgc cgcgcatcac
     7261 gctcaccacc ctgctcggct tcgccagcac cgacctgggc cggctcttcg acagcgaccg
     7321 ctacgcctgg tcgctgcagc cgacgctcgg cctgccgctc ttcgacggcg gccgcctgca
     7381 ggccggcgtg gaggtggccg aggcgcagca gcgcatcctg gtcgagcagt acaaggcgac
     7441 gatccgtcaa gccttccgcg aggtcgccga cgcgctcgtc gccttcgagc gcctcaccga
     7501 gcagcgcgcg gccacgcgcc gcctcgtgca cgccaatcgc gagcgcctgc gcgtgagcaa
     7561 ggcgcgctac ctcgccggca tctccagcta cttcgaggtc atcgacgccg agcgccagct
     7621 cttcgacagc gagctcggcc tcgcgcaggc cacgcgtggt gcgcagcagg cggtggtgca
     7681 gctctatcgg gcgctggggg gtggagcggc ggagtcgagt gcgtcgaaca cctagctggc
     7741 gccggtcgaa cgctgcgccc tcgacgatcc agcggagctt cgaaggctgc ggtgttccct
     7801 cggacacgtc cgtccgcgtg tcttgacatc tgctcacctg tcaccttgat tgaaagcgga
     7861 tggcgtcgat ggtggtcggg atatgcgaag ctgcttcccg gcgaggacgg atggatcttc
     7921 tcgagaggag cgcgacatgg ccgcatactg ggatgaagcc gcgatgcagc gggcgctggt
     7981 gatgccgagc atcctggcga tcggagattc ctggttctgg tatccctttc ccggaggcag
     8041 cctgatcagc tacctcggac cgatcgtcga gaagaaggag cacaccatcc tcgcgcaggg
     8101 aatgaacggg gcggaggcct tcgactacgt cgatggcaag tacaaggacc ggatcaccgg
     8161 tgcgctgcgt cgatatggca gcagcctgtc ggcggtgttc atcagcggtg gcggcaacga
     8221 cttcgcgggg ttcaacgaca tgcggcccct gctcgccttg gactgcagcg gcgcgcagtc
     8281 ggctgccgac tgcttccgca gcggggacgg cgggctcggt cagttcctgg agcgcatgga
     8341 cgatcattac cggcgcctga tcggcctgat ctacacccat acctcgctcg actgcctgat
     8401 cctgatgcac tcgtatgact atgcgattcc cgatggtcgc ggcgtcgcgg gcaaggccgg
     8461 ctggctcaag ccggcgctgg tcgacgcggg cgtgccggaa gcgctccagc gtgactgcgt
     8521 gcgctatctc atcgatgcgt tccacgccat gctcgggcgc atcgcgatgg gcgatccggc
     8581 gcatctgcgg atcgtcgaca gccggggtac gctcgcggaa gaggattggg cgaacgagtt
     8641 gcacccgaag ggcaagggct tcaggaagat cgccaaggaa cgctggaagc cggtcctcca
     8701 gggggcaggg ctcgcctgag cggttcatgg atggcggaac ttgcgatcgg gggccgatca
     8761 catggggcgt cagctcgcgc cggagtttcg ccgggaccgt ccctcgtcca tccggtcttc
     8821 gcccttgcga tgacgtcgat cgacgggttc atcgccgcct cgctcagcag cccggcgcgc
     8881 tgagcgggag gggggaaagt tcggcaaccc ggtgcgcgca aggcgacggt gccgtatgcg
     8941 ggcgaacctg caccgccgcg aacaggcgat gcccgctcga gggtcttcgg gacgagggtt
     9001 gaatcgtgtg gcgcaacaaa aaagcgcccc gaaaggcgct tgattgcagg tgttctggcg
     9061 gacagggcgg gattcgaacc cgcgttggga tattatccca aacacgcttt ccaggcgtgc
     9121 gacttaaacc actcatccac ctgtccggtg aagcgcggca ttatagccgc aggggcgcgg
     9181 attagaaagc gctttttgtg cgcggggcgc tgggcgctgc ccgcccggaa tcggtactcc
     9241 tcaccgccgc agcgcttcga gctgtgctgc gacttccgcg gcgagggcat cgagttcggc
     9301 gtcgagttcg gcgagcaagg catggtcccg cgagaggcag gcttcgggat ttgccttgca
     9361 gtgcgcctcg agcttcttca ccagggccac cgccggcgcg gcgcgcagct gcgcgagggc
     9421 gccgcgcagg cggtgggcca cgcgacccaa tggctcggct tcagcgctct cgatggccgc
     9481 gcgcagctgc tggcgggtgt cggtcagcca gggggcgaag gtctccagca cgacgcgcac
     9541 ggcggcgcgg ctctcggaca tgtcgctgcg cagggcggcg aggtcgaagt gggaggtgga
     9601 ttcgggtgtg tgggggggca tgtcggtctc gcagctcagt cgttcggtgc gtggcccact
     9661 gcggcgtgca gcccgccgat cgtgatcggc ttggtgatga cggcgtcgaa gccgcccgcg
     9721 aggtagcgct ggcgctcggt ctccatcgcg tgggcggtgt aggcgatgac gcggacgccg
     9781 ttccaggcgg cattggcgcg gattctcctg caggcttcct cgccgccgag gtcgggcatg
     9841 gagatgtcga gcagcatgaa gacgggctgc acctcggcga ggcgcgcgag catcgggatg
     9901 gcgctgtcga aatcctccac gacccaaccc agccggcgca gcagcaggcg ggcgaccgcg
     9961 acgttgaccg cgctgtcgtc gatgatgaag gcgagcttgt tcatgatgga ggaccgcccg
    10021 cgggggcggt gaagtggcgc gggaggcgca gggtgaaggt ggatcccttg ccggggacgg
    10081 agtcgagatc gatcgtgccg cccatccggt gggcgagccg acgcgacagg gtgagtccga
    10141 ggccggtgcc gccgtgcccg cggttgagga aggatgcggc ctggtagaag ggtgcgaaga
    10201 cgcgctcctg ctcgtcgcga gcgatgccgc agccggtgtc ggcgatgcgg atcgagatct
    10261 cgtccgcggc ggcggcgagg ccgacgagga tatggccttc gcgggtgaac ttcaccgcgt
    10321 tcgacagcag gttctcgatg atctggcgca ggcgcaccgg gtccgcgtcc acgatgcacg
    10381 gatgggcggg cagatccagc cgcaggtcca cgcccttggc ttcggcgttc accttgtgca
    10441 gcgcggccac cgaggcgacc agggggacga ggtcgatcgt ctgcgcgacg agggtgagcc
    10501 ggttggcctc gaccttggcg aggtcgagca cgtcgttgac caccgcgagc aggtggcggg
    10561 cgctgtcgtg gatggtggtc gcgcactcgc gctgctcggg gtcctcgagc tccatgctga
    10621 gcagctcggc gaagccgatc acgccgttga gcggggtgcg cagctcgtgc gacatgttgg
    10681 cgaggaactc gctcttcatg cggttggcct gctcggcggt gtggcgggca tcctccaggc
    10741 tgcggatcga gcgttgcacg gtgtcggcca tgcggttgaa ggcgcgggcc agttcggcga
    10801 gctcgtcgcc gcccgcggcg gccaggcgct ggtcgaggtc gccctgctcg aagcggtgga
    10861 tgccatcgac cacgtgggtg atccggcgcg agagcagtcc ggccatccag atcgcgatca
    10921 ggatcaccgc gagcaccatc gcgagcgtgg aggcggccag gcgggtggtg gtcccgcgca
    10981 gctggtctgc gatgtcgccg accagggtgt cgcgctcgtg ctgggtgagc gcgtcggcct
    11041 cgccgatcat cttgccgatg cgctggcccg attcggtggc cgcgcggtgg aagtcgtcga
    11101 cattggcgcc gatggtgacg tagccgaagc cgcgcggcgt cctgccgtac tggccggtgt
    11161 agtaggggat ggtggcaacc gtggtgagct tgcgcagccc cgagaacagg atggtgaagg
    11221 agcccgagcc gccgtgctcg gtcatgtcgt gccagccctg gcattgcggc gagaagtcga
    11281 ggtagcggca gtcgagcgcg accaggcccg aggcggtgga ttcggggtgg ggctcccggg
    11341 tgaggcgctg gtcctgataa ggcgtcaggg cggcggagaa ggtcggccag gaccgtccgc
    11401 tgctgcgcca ggcttcgtag agctcgcggt cgagccaggg cacgctgcgc tcgccggtgg
    11461 cagggtcgta gccgtggatg aagtagtcgc gcgcgtgcga gatgttgtgg ccttcgtcgt
    11521 cccagatgaa ggcgtagttg cccgaggcgg ggtcggcgat cggggcgaag cgttcgggcg
    11581 tgggccgcag ggtgtcggtg aaggccatca ggtgggcgtg gtcgagcgcc agcgtgacgt
    11641 agccgatgat cctgtcctgg cgcagcaccg gggccgccca ccggatcaat ccccggaagc
    11701 gcttgccgac cgggttctcc agtccggcgt agccggagtc ctcgggggcg aagggtttgc
    11761 cgagggcttg cgcgcgagtg ggggtgtagt cgccgatcca gtgcgtgcgc acctgggcgc
    11821 cgatcacgcg cgagacgtgg atctcgccgg ggcgcagggc cttcagtgcc ggccagtagg
    11881 tctcggcgcg cacgaaggtg ttctcgcgcc gggtgatgtc gcgcaggccg gggtcgagga
    11941 cctggcggcc ggtctcgctc agcgccttga tgcgctcgtg gccgtcgcgg tcgatgaagg
    12001 tgatctcgag gaagagcggg cgcggggtgc cgcgcatgtg ggtgtcgggt gcgcggtaat
    12061 ggaagctgtt ggagttgtcg ggcagcggcg cgcgcaccaa cgcgagctcg cgcggctgcg
    12121 ggtcgcgctc gacccagcgc tcgccctcgg cgtcgagcgc gtagggcccg tgctcctcga
    12181 tgttgcgcac acgggtgtcg acgaagctgc ggtaggcggc cgcgtccgcg ggcaggctgg
    12241 cggccgcgcg gatgtcgccg tcgcgctcgt ggaggaactg cgcgatgctg cgcgcggtgg
    12301 cggtggtgag cgtttcgatg gcctcgcgcg agcggtcgtc gagtgcgtcg atggcgtcgt
    12361 cgatcgcggt ctggccggtg cgcatctggg tgtcgcgcat cacgtcggac atggtgatcg
    12421 cgcgctcggt gacgagccgg cccagatcgt gcgccgcctt ccaggcgaac accgcgagca
    12481 gcaccagcgg caccaccttg atcgcgatga agatcgcgat cagcttggcg cgcaggctca
    12541 tcccgcggat cgcgcgaggg gtctccatcg agcaggctcg cggggcaggc ggcgctcagc
    12601 ccgggtggcg agcgcggcgg cgcatcgaga tcatcgcctg gacgtagccg tgcagctcgt
    12661 ggaagggaat gggcttgcga tagacggtga tgtcctccgg cagtccaccc gccgcggcga
    12721 tctccttggg accgagcgcg gacaccacga cgatggcgat gtcgctcgac agggggttgt
    12781 cgcgcaggcg gcggatcatc tcgaagccgt cgatgcccgg gatgcgcagg tcggcgatga
    12841 gcaggtcggg cggctcgcgg ccgacctcga cgagcgcatc gaggccgtgc tccatcaggt
    12901 gcacgtcgag cggcaggctc caggtaagga aggtctcgcg gtagatgttc tgcagcagtc
    12961 ggtcgtcctc gaccaccatc acgcgcaggc gctcacggtt gctgcgcatc ggtgccgagg
    13021 gcgagcgcgc atgcgcgagc agggcgtcga cagagtcctg gcggatgcgg cggtggccgc
    13081 cggcggtctt ccagcctgcc agcgttccgc tttcgaccat ttgctgcacc gcaccgagcg
    13141 acaggccgag ctggcgtgct gcctgggctg tgctgatgaa ctcggaattg gagtcggact
    13201 ttccagccat gggcgtctgc gattcgaggg aaaagttaaa agtaccagaa ttatggcagt
    13261 ctgacgtggc gatgcgaaag gaatttcaag gatgcgggcc tcgacccggc gggggtgttc
    13321 cgcgcacggg acgggccgct acaatgatcc ggacgcggtg ggccgcgggc gccgcgggcg
    13381 ttcgccgatg ccgctacgaa agacttccgg atcgccgacc atgaacacct cgaagaacgt
    13441 gctgggcgga gccttgctcg cctgcagcta ctcccccctg accggtttct accgcaccgg
    13501 atgctgcgag accgggcagg acgacctcgg gcgccacgtc gtctgcgtga aggtcgatgc
    13561 ggccttcctc gagttctcgc gacgggcggg caacgacctg tcgacgccgc gaccggagta
    13621 ccgcttcgcc gggttgaagc cgggcgaccg ctggtgcctg tgcgcgctgc gctggaagga
    13681 ggcgctcgag gcgggcgtgg cgccgccggt gatcctcgag gccacccacg aggcggcgct
    13741 gcgcgtgatc gacctcgaga ccctcaaggc ccacgcctac ggcgcggcgc acggccccag
    13801 cgcctgatgg cgagtgcgcg cgcgccgctg cgcgagcggc tcgccggctt gctcgccgtg
    13861 ctcgaggcgg gcctggtcga acgccggcag gtgctgcgcg cgagcctgct cgccgcgctc
    13921 gcgggcgaac actcgctgct gatcgggccg cccggcacgg ccaagagcgc gctcgcgcgc
    13981 cgcatccacc tggcctttcg cgatgcgcgc tatttcgagc gcctgctcac ccgcttcacc
    14041 gtgccggagg agctcttcgg cccgctgtcg atccgcgcgc tcgaggaaga ccgctacgag
    14101 cgccacgtgg cgggcttcct gcccgatgcg gcggtggcct tcatcgacga ggtcttcaag
    14161 gccaacagcg cgatcctcaa cgcgctgctg acactcctca acgagcgcga gttcgacaac
    14221 ggcgcgggcc gcctgcgctg tccgctgatc agcgtggtgg gggcgaccaa ccaggtcccc
    14281 gaggacgagg tggcggaggc cttcttcgac cgcttcctgc tgcgcctgac cgtggcaccg
    14341 gtgagcgcgg aggggtttcg gtggctgctc ggcgaggatc gcccggggtc gcccgaggta
    14401 ctcgcgccgc cttccggact gcagctcgac gaggccgacc gcgcggcgct ggcgcaggct
    14461 gccgccgctt gcgcactgcc tgcggcggtg ctcgaccgcc tcggcgagct gcgggcctgg
    14521 ctggcggggc gtggggtgta tgtatccgac cggcgctggg tcaagctcgc ccacctgctg
    14581 cgcgtggccg cggcgagcga gggcagggcg gcggcgagcg agtgggatct gggcctggcg
    14641 ccgtgttgcg tcgctgccga cgagcccacg cggctggcgg tggcggactg gctggcggcg
    14701 cgtctgggga tccgcgaggt ctggtcgccg ccgcgcatca cgcgggtggt ggaggccttc
    14761 gaggctcagc tcgccaccga gcgcgaggct cgggatctcg actacgacga gcgtgggcgc
    14821 ttgcgcttct cggcgatcga cccggtggcg acgcaggccg gcgagctcgc cggctcgatc
    14881 ggcgacgcca agggcggggc ggcggcgctg cgcatgagcg ccgcgcgcat gcggcgctat
    14941 ggcagcctgc acatcggtgc gcgcacggcc cagctcgacg gcgtgctcgc gcgcctggcg
    15001 gcgtatcgcg gcgaactcgg cgcgcagcgt gcgacgctcg aggcatgggc ggaccaggcg
    15061 ctgtgggcgg accctgcctt cgtcgcctgc gcgcgttccg ggctggacgc ggtggatgcg
    15121 gtactggccg ggctcgaggc ccgcgcccgc agcgcgcgtg ccggcttcga ggcgttgccg
    15181 cggctggagc aggcaggcgc ggcgcccgcg ccggtcgagc acgacccgct gccggagtga
    15241 ggagggggtg atggcgaccg gcgatgcagg ttccggcgtg gcgcccgccg tcgcgcagca
    15301 atcggcctcc gcggcggccg acagcgcgcc cgcgccgctg ccgccggacc gcgcccgggc
    15361 cgccgaggct ccacccgtcc cccggctctt cgaggcgctc gaagccgtgc tcgcgccgct
    15421 cgatcgcctg ccgcgtccgc tctggctcgg tggcatgacc cactccggcg gcgaactcgc
    15481 gggccgtctc gccgcgctcg aagcctggcg ggctgcgctg ctcgccggcg gcctgcccgg
    15541 gtcttgcgcg gcctggcccg aggcggaggt gcaggaggcc gtgcgcgagg tcttccgggg
    15601 cctgggcttg cccgcctact gtgcagacca gcccgcgctg gtcgataccg tgctgcaagg
    15661 cctgctcttc cacctcgacc tgatcgtcga ctaccgcgac cgcggcgata ccgaggccgc
    15721 ggcgcaggcg agggcgctcg attccttcgc tgccgactgg gccgagcgct gcggcgagat
    15781 cgacgagctg gtcggcgcct tcggggatct cggcgatctg ctcgacaacg cccgctggga
    15841 tgcgctgcgc ggcctgctgc gcagcacgga ctggcgcgag gtgctgcgca tccgcgcgct
    15901 gatcgagggc ctgcccgagc tcgcccgcat cctgcgcgca ctcggccgcg cctgccccac
    15961 cgacgaggac gccgaatcca gccgggcatt gcacgccgtg gtcgagcaca ccgagataca
    16021 gcgcagcgtc tcgcaccggg tgcgggtgcc cgacctgccc ggcgaaacgc gcggcgtgca
    16081 gcgctcgggc cggatcgcgc gcatgctgcc ggccgaggcg acgctgctcg gccatccgcg
    16141 cctgcgcttg gtctggcatg cgcgccgcgc cgagcgcacg ctgctcgcct acgaggacga
    16201 cgaccacctg caggaggact gcctgcgccc ggcgccggtg ctgcgcccca gccagcgccc
    16261 cgcgccggca cggcgcctgg agcaggggcc gatgctggtg tgcgtggata cctcgggatc
    16321 gatgcagggg ggcgccgagg cggtggccaa ggcggtcgtg ctggaggcgg tgcgctgcgc
    16381 tcacgcccgg cgccgcgcct gccgggtgta tgccttcggc gggcccgacg aggtggtcga
    16441 gatggagctc ggcgtcgatg tcgatggcgt cggccggctc gcccgcttcc tcggccaggg
    16501 cttcggcggc ggcaccgaca tctgcgcccc gctcgagcgt gcgctcgccc gcctcgacga
    16561 agccggctgg cagctcgcgg acctgctgat cgcgtccgat ggcgaattcg gcgccacccc
    16621 ggcgctcgcc gcccgcgtcg aggccgcccg ccgcgagcgc ggcctgcgcg tgcagggcat
    16681 cctgatcggc gaccgcgaga ccatcggcct gctcgaactc gccgacgaca tccactgggt
    16741 gcgcgactgg cggcgctatg gaggcggtac ggacaagccg ggtggggccg acgcggacaa
    16801 gcggcgtggc gatggcgccg gcgcgaacgc cccgaccctt gctgccggcg gtggctcgcc
    16861 ggtgcattcc agccacctca ccgcggacta tttccccggt gccctgcgca cccccgagaa
    16921 ccgtgccgcc accgtcacac ccgaggcggc tgccgctgcc atccgtgccg gccgccaccg
    16981 cggcgaccgg ttgtaggagc ggcgcacgcc gcgaacgtcg gccttcgggc aatccccgcg
    17041 cgtctcgttc ccgcggccgg attcgcgcct tgcggcgctc ctgcaggggc gtgccgtcgt
    17101 ggtaggctcg cccgacccca ccgcccggcc ttgcgccgct gtccatcatg agcttcgcca
    17161 ccgacctgat cgccgaactc gaacgcgcca ccgccggctg cccgatcccg cgcgtgcgcg
    17221 ccctgcacct gccgccgccg gaggcggccg catcgaagaa cggcgagttc tgcgcgctcg
    17281 agctcgacga cggcgcgctc ggcctctcct atgtgctgct cggcggcatc ctcccgcggc
    17341 tcgccgcctc cgacgacccg cacaccatcg tcggcatgga cgcgctgcag ctcgcgcgcg
    17401 agttcgccgc gcccgccgcc ggcgcggggg gcgacgcgga gatccgccgc acgctcggct
    17461 tcgccgcggc caacgcgctg tcgcgcacgg tgatggagcg catcggcttc gcgccgccgc
    17521 gcgccaccga ctcgatcggc ggcatcgacc cgcaggcggg cgaccacgtc ggcatggtgg
    17581 ggttgttcac cccgctgctg aagcaggtca ccgctgccgg cgcgcagctc accgtgatcg
    17641 agctgaaccc cgactatgcc ggccactacg acggctaccg cgtcaccctc gacgccgcgg
    17701 cgctggaaga ctgcaacaag gtgctgtcga ccagcaccat cctgctcaac cacacggtgg
    17761 acgacatgct cgcgcattgc cgcaatgcgc ggcgcatcgt gctgatcggc ccgagcgccg
    17821 gctgcctgcc cgatccgctg ttcgcgcgcg gtgtcaccgt ggtgggcggg agctggatca
    17881 ccgatcgcgc cggcttcatc gacgccctgc gccggggcga gtcctggagc ggcttcgcct
    17941 acaagttcgc gctcggcgcg gcggactggc caggcctgcc ggaaaaccgc taaggttgcg
    18001 gcctcgcgtg cgggcagcct ccacgctgcc cgcaaaacaa taaagatcga ccccccaaat
    18061 gcacctgact ccccgactcg tcttcttcct cgtcctgccg cccctgctgt gggcgggcaa
    18121 tgcggtcgtc ggccgcctcg ccatcggcag catggacccg ctctggctca acgcggtgcg
    18181 ctggatgctc gctttcgtgc tgctgctgcc gctcggctgg cgcgcgctcg gcacgccggc
    18241 ggcacgtgcg cagatccgcg cgcgctgggc ccacctcgcg gtgctcgggc tgatcggcgt
    18301 gggcgcgtac aacgcgctgc agtacatggc gctgcgcacc agcacgccgc tcaacgtcac
    18361 cctgatcgcc tccagcgcgc cggtgtggat gatgctgatc ggcgccgtgt tctaccgcgt
    18421 catcccgcgc ccggtgcagg tcttcggcgc gctgctctcc ctggtcgggg tggcgctggt
    18481 gctgtcgcgc ggcgacccgg ccgcgatcgc gcgcatcgag ttcgtcgagg gcgacctgct
    18541 catgctgctc gccatgctcg gctggaccgg ctacagctgg ctgctcgcgc gcccgcccgc
    18601 gcacatggcg ggcgaggcgc ggccgaagtg gaactgggcc gagttcctgg tcgcccagtg
    18661 cgtcttcggc gtgctgtggg cggtggccgc cgccgcggcg ggcgacgtcg tgttcccgtc
    18721 cgggcccacg cagtggtcgt ggggcctggt cgcggccatc gtctatatcg ccgtcggccc
    18781 ctcgatcatc gcctaccgct cctggggcgt ggcggtggcc gaggccggcc cggccaccgc
    18841 ggccatcttc tacaacctca ccccgctctt cgccgcggtg ttctccgccg cggtgatcgg
    18901 cgaatggccg cgcccctatc atggcatcgc cttcctgctg atcgtgggcg gcatcctggt
    18961 gagcacccgc gcagcggcgc ggcgccaggc ggcgtgagcc ggcgcgggac ggtgcagtcg
    19021 gtcccgtgcg ccttcaccgc gcgcgcgtca atagtccgtc tcgacctttc ggccggtgcg
    19081 gcgctcgatg taccagccct tgcgcacgac cttgccgttg acgatttcgg cctcacggaa
    19141 atcgcccccg gcgaaggagt ggacggcggg aaagtggatg tcccggcctt gcgggtcgcg
    19201 caggttcgga tcgtagcccg gccggcgctg gatatcccag ccgaacagcg tgccatcctt
    19261 gctcatgcgg aacttgagcc gcagattgcc gcgcggatcg cgctcgaggt cgtcgatgag
    19321 ctcctgcggg atgcgggagc cgacttcgat ggtttcgtcg ccgaggatgt tgccggcgta
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgtgccgag ggccgccacc
    19501 gcgagccgga tcaggaagcg tcgtcgcatc agaatcctcc atttgtcgtc gtgtagccgt
    19561 tctcgggctg cccgagccag gcgaggtagc tcgccatatc caccgtgccg gtgacgtcgc
    19621 cgagacggcg cgggtcgagc gcggagccgt cgccgcgcct tgtggcgtcg cgcgggagcc
    19681 aggtgatgta gcgcagggtg tcggcgtggg tgaggctgcc gttgtcgaac gccatgcggc
    19741 gctggcgatc tccggccacc gcgccgttga ccgcgcggtc ctcggggaag acgtaatcga
    19801 tccggggcag atcgccgttc ccggtcgggc gcggaacgac gctgtgttgc gggttgccga
    19861 tctcgatcac attgctctgg tcgtggatca ccacattaag gatgggaaca gggtccaccg
    19921 attccatttc tactccggcg tcgcgtgctt gcgccaccat ccagttgagc gcaaccagcg
    19981 agaggccttg ctcgacgtcg ggatagcccc cgccgatgtc cgcatgggcg ccgacgaagc
    20041 ccttctcgat gcggatgcga ccgggcgcag cgctgctggc gccgatcgac tcgagcggaa
    20101 agccgcccca gtgcatgcgg tgcggcttcg ggttgcgata ggcgaactcc gtgatcgagt
    20161 ccgagcggtg ctcgttgagc gcgaccgcct gcgcgacatg ggcgaagacc tccgggatcc
    20221 cgaggcgata cgcttcgccg ctgaaattgg tcgatagcac ggtgtcgaag agccccatga
    20281 agcggaaatc gaccgcctga caggcagagt ctccgcggcg gttcgtgtag cggtagtagt
    20341 cctggccctc atggcggacc gtcttcgcga cgatccggtt ggcgaactcg cgcgcctggg
    20401 ccgcgccgcg gctgaagccg acgatgtcga tgtccatgac ccggtcgtca ctgaagagct
    20461 cggcctcgtc gtccaggtat tggctcatcc gcccgatccg tgccggcccc gagtagttgc
    20521 cgcccatgtc gttcacatgg gccggatcgc ccggtgtcag ccaccacagc agatggccgg
    20581 tcgcgtggtc ttccgggcgg atgtcgccgt agtcgatgtc gtgatgcacg gtgccgacgc
    20641 cactgacata gcgcttggtg gcagtgtaag cgtcaaagaa atacaccaca ttgctgaatc
    20701 cgctgcccgc catcgccggg tcgttgaggt cgtcgctgtt ccccgtccca tcgaacgcga
    20761 acagcaccag cccatccgga tccacgttcc gcagcggatt gaacgccgca tacgcatacg
    20821 ggttcggccc gtccggcgta ccgagcgggt cgggggtgag gtattcgccc agggtcggcg
    20881 cgtagtagcg gcggcggttg tagtgcaggc cggtttcggc gtcgaagacc tggccgggca
    20941 ggcgcaggtc gagggtgaag tcgggacgcc ggggcgaggt ggtgaccgtc gcggcgccga
    21001 agggggcgtg gcgcgcgcgc cagagcggct cgccgtcggc gtcggtggcc agttcggggg
    21061 cgccgaggtg gttggtgtgc agccaggcga ggccggcctg cggggcgagc cagctttgcg
    21121 cgatgcggcc gaggtctgcg aggatgcgtc gcgcggagtc ggttgcgctg tcggggcgcg
    21181 ccggggtgtc gagcacggcg aggggcaggt cggcgagcca cagatactgg cggatcaatc
    21241 tgccgtcggc gtccagctct gcgaggggtt ggcgggcgtc gtcgtacacg gtgtgggtcg
    21301 tggtgggcgc gaccattgcg ggatcgaagc gcgtgcgcgc gatgcgcagg ccgcggtgat
    21361 cgtaggcgta gcgggcgatg ctgcgctcgc ctgcgcgcac ctcgatcagg cggccgagtg
    21421 cgtcccatcg caggctgcgc gcgccgaagc gttcgggctg gccgctggcg tcgtggtgac
    21481 cgggcgtggc ggcctcgtgc ggtcggctcg cggcggcccg cgccggcacg tttgctgcgc
    21541 gtgggctcga ggacagggca cggtggctgc ccggtgcgaa gtgggtcggg cgggtgtggc
    21601 cggcgagctc ctgctgcgac acggcgcctt cctgggcaag gacgcggcgc tggtggcgat
    21661 cgtaggcgta acgccacacg gcggtttcgg ccagcacgtc gccgtcgtcc cgccattcga
    21721 cgctggccac gagctggttg cgaaggtcgt aggcctggct gcggcgcgtc gagggcagcg
    21781 ggccgtcggg ctcgcgccgg cgcgcgtgca ggaggttgcc ttccgggtcc cagacgtact
    21841 ggtagtcgag caggatggtg tcggcggacg cgacagtctc ctgcagccgt ggtaacgcac
    21901 cttcgagggc ttggccatct gctgcctcca ccggctgggt gacgtccgca tcggtgtttg
    21961 cttgcgcggc gtgggccgcg gaaatgccga acaagcgttc gatgcgcgtg gccaggggct
    22021 ggccaggtgc gatcggcagc agcgcacttg cattacggac cggtgcgccg cgctcgtcgg
    22081 cgcgcagatg aacgacgcgg agcaaggtgc cggtgcgcgt gcgctcgtgc aacgtacgca
    22141 tgccgttgcc ggcccggaag ccacgcaggc cgaagagatc gcgctggaac gcgctggcga
    22201 tgacctgttc gcgcgccagc gcgcgcagcc agggcgtggc caccggctgg cgcttcagcg
    22261 cgacgacctg gccctggccg ttgcgctcgt agcgcaggcg actgccgtcg ggcagggtgg
    22321 tggcgacaag atgcccctcg gcgtcgtact cgtagcgcgt gaccacgacg agctcgccgc
    22381 cgtcgcgctt caggctcacg atgcgcgcgc tgcgcaagcc gcgctggtcg tactcgtagc
    22441 gttcgcgccc attggggtga tcgacttcga gcaggcggga gccgtcgtag cgccagcggg
    22501 tggtctcggt gctgccgttg cgcccgtcgg tgacctcctg ggcgaggatg cggccgcgcg
    22561 ggtcccaggt gtagcgcgcg cggtggccga gggcgtcgcc catggcgaca aggcgccccg
    22621 cggcatcgaa actgcgccag cgcgctccgg cgtcgggact gcgttcgagg acggggcgtc
    22681 cgaagtcgtc cttcaggatg cgtgggtggg cagggggtgc tggcggcgcg gatccgccgt
    22741 cggggtggcg cgcgggcggc agcagctcgc gaccgtgact gtccaggagg ccgacgagcc
    22801 ggcctgcgac gtcgtggcgc aggctgcggg tgaagccggt gtcgtcgtcg atcgcggcga
    22861 ggcgtccgtg ctcgtcgtaa cgccagcggc gccgcagcag ccggctggcg ctgcgcagcc
    22921 cggtctcgac gacgcggctc tcgtggtcgt aggcccaggt ccgcaggatg ccgagcgcgt
    22981 cggcgtgcca ctgcaggcgg ccggcggcat cgaaggcgcg ccgccagcgc ggatgcaggc
    23041 gctcttcatc ctctccggag cgcagctcga cgagttggcc gagggcatcg tattcggcag
    23101 cgtggatgcg atctgccgcc tcacccgcgc tgcggctgcg ccaccggatc agctggccgg
    23161 cggagtcgtg gcgcaagtgg gtctcgtgac cttcggcatc gcgcacccgc cgcggcaggc
    23221 cggtggccgg atcggattcg atctcgctgc gctggccgcc gggaccttcc agcgcgcgaa
    23281 tgaagctgcc gcgttcgtcc cagtgcaggc ggacgaggtc gctgtcgagc gggctggcgt
    23341 ccgctccgtc ggggagcggg ccgtcgagct cggcaagcac gctgcgaccg ttgatcgtgg
    23401 cgtagcgcca ggtgacgcgg cgttcgatcg gcacgggatc cggccggcct tcggcgtcga
    23461 tcgggctgaa accggactcg cgcagttcga tgggctggcc ggtcgcgttg tagcgcagct
    23521 cgatgcggtg ctcgcggccg gggacgacgc tcgggcggat gatcgcgcgc ggaggcgtcg
    23581 tcgcggcggg ctcggtgcgt tgcgctgcac ggtccgtgtc ggcgagcgtg gttgccggcg
    23641 tgtcgggata ttcgtagcgc gtgcggtcga gcaggcgcgg ccgggcgccg gagagatcgg
    23701 caccgagggt ctcggcgagg cgcccgcggg cgtcgaaacg gtgctgggtg gaaaggcgcg
    23761 gcctgccctc ggcgtccagg gtcgtgatgg cgctcaatcg gccgtcgggc gtgtagcggt
    23821 agcggacgtc ggtggggccg cagcgcgcgc agcccgggcc gcgcgcttcg agcaggcggc
    23881 gctggccggc gatcaccgcg ccaaggtagg tggtgcgctg gccgaggctg ttggtgagca
    23941 cgctgcggcc gcgttcggga tagtcgaggc gcacgtcctc ggggccgctt tcaccgcctg
    24001 ccggcagcgc gccacgcacc gagcggatgc cgcgcccggc gtggtcgtag gcgtagctgg
    24061 cgatgcgttc gggcacgggc gggcctgcgg gctgacgcgc gagcacgctg atgccggtga
    24121 gcgcgaccgg atggcgcggg tcctcgtagt gatattcgcg cgcggcggct ccgccggtct
    24181 gcgcagccgg tgcgccgggg tagtcgacac ggagcaggcg cgccgcggtg accgcgggtg
    24241 cagcggcctt tccggcggga gcgccgcgct gcagggggcg gctcgggccg gcggagcccg
    24301 ggcgttgcgg agcgtggacg gagcgcccag gctgcgtgcc tgcgtccgtc cgcgcgggaa
    24361 gcgcgacgcg ttcctgcgca tacgagaagc gtccgagcgg actgtcgatg tgggtgatgc
    24421 cgtcgatgcc gctgccgggc gcgtggtagt gaacttgcag gctgcggccc tgcgggtcgg
    24481 tgacctgcag cagccggccg gcggcgtcgt ggcgcagtga gacgaactcg ccgctcgcaa
    24541 cgaggatctg caccaggtgg ccggcgctgt cgaacagcag gctgcggccg tcgggccagc
    24601 gccagagata ttcgtcgccg ccgccggggc ggtgccggag caggatctcg ccgcggctgg
    24661 ggtcgcggct ggcgcagcgc tgcggctcac cgggccggcg cgcgaagatt acccgcgcgc
    24721 cgtcggcctg gaggatttcg aggcgatcgt cgcggatgtt caggcgcgtt tcgtaggaca
    24781 gtctccagcc gctgccgagc gccccgatgt cgcccgggcg cgcacgtcgg ctgttgtagt
    24841 ggcggacgat ctcgaggccg aggatgcctg gcagcgccgg aagatcgacc tcctgttggt
    24901 acttgttgcc cgagaggacg tcgatcgggt ttcccgcgcc gacgtcctgg ccggtggtgg
    24961 aaccctggct ggccgggccg ctggcgctgc cgcaggggtt gcccgcgttg ttggagacgc
    25021 attgccccgc cgcggggggc tctggcgccg aggggccgtc ctgcgcagag tccggcgcac
    25081 agctcatcgt gattccgcac ccgctctgct ggatgccgtg atccgcggcg ccggattgcg
    25141 ccgccgaggt ctcggccgct accccgggga gcgccgtgat acagagcgcc gccagcagtg
    25201 cggcacccgc ccgcatcgca tggtgcagcc gaccggctgg cggctgcagg catgcggacg
    25261 agctgcgcat cggttgccgc cgcgccggag tggtccggcg gtacaaggcc gcgggggcat
    25321 cgcatgcaag cgtgggggca gcgttcagca ctcgggttcc tcctccgcgt cctcggcgtc
    25381 ctccgcgtcc tcggtttcgt cgccgctgtc ggttcccggc cccggctcac cgtcggctcc
    25441 gctgccgtcg ccagccggcg taccgtcttc cccggtcggg tccgccgcct cgggtgcagt
    25501 gtccgcggtc gcaggttcgg gcgtgcacag aggggccgtg ggggtgcagc cgggcagggg
    25561 cgtcgaatcg gcggctgggg gggtggcgct tcccgccgta cggggccgca tcgcctcgtt
    25621 ctcgatcgcg tcggactgca tgcgcagcgt gacctggctc accatcggga tgcggccggc
    25681 ctccaccagg ccgcggcgga aggcgtcgtc gtcgtccgga tcgagcatgc gcagggccca
    25741 ggtgaagaat cgtccggcga ggggaacctg cttggccggc ggcagccccc aggtgacgcg
    25801 cagcttgagc aggttggcgt cggcgagtgt ctggcccgag cggttgcttg ccggatcggc
    25861 gttgcagttc gcgcggtccg ccgggcagcc gagcacgccc ggctgcgcgg acggaatcac
    25921 ccgcgccgtg gtctgcaggc ggcgcgcggc cgccaggctg tggtagtcgt cgaagctctc
    25981 gcgggtgggg gagacgatct cgatgcgcag ggcggcggcg agatcgagct gcgccgcggc
    26041 gaaggcgtcg gcgagctcgg cgaggttgcg accgccgccg taataggggg tcatcgcgcg
    26101 cgcgaaggcc aggcgcatgc tcgccagtcc ggcgttggcg atgcttccgg cgcgcgcggc
    26161 ctcgaagctg gcgtaattga cgatgctgcg ggcatggaag gcgagcccgg tctgcagcat
    26221 cgccagtgtg agcgcgagca gggccggggc gacgatgacg aactcggccg cgccggcgcc
    26281 ccgctggcgg tggcgccaag gccgtggaag aagccgtgga agaggccgcc gcgccggcgg
    26341 cagcggatgg gcgggattca tcatgcgcgc actaccaccg acaaggtgga agccagcctg
    26401 aaatgcgtgc cggggacgag tcccgcccgt ctcgcggcgc cggccgccat ctcgagcacg
    26461 gcggcggcgc cgcggcaacg cgcgacacgc cacgggccga gcgcctcgcg caccgcgaga
    26521 acgacgcctt gcgggtcgat gaacaccacg tcgatggcga agcacatgcc gaaggtgtgg
    26581 accgacgagc atggacgaag cagcaaggcc tcgtcggcgg cgaggtgcgt gcgtccgagc
    26641 aggccgcggg cgcgttgcag gaagctgcgg gcgacaagga cgcgcatgct cacagcccca
    26701 tggtcatgaa cttcaccgcg atcggaaagc cgagcacgat gaaggtgcag gggaagatgc
    26761 aggcgatcag tgggagcagc atcttcacgg gcgcttccat cgccgccttc tcggcgcggg
    26821 cgaagcgctc cgcgcggcgt tgctcgccct gcgcgcgcag catcgggccc aggctcatgc
    26881 ccatgttctc ggcctgaatg accgtggaga ccaggttcgc gatcgccggt tcgccgatgc
    26941 ggtcggcgaa accgcgcagc gcgtcggcgc gcgacttgcc cgcacgcacg tcgcgcagca
    27001 cgcggtgcag ttcttcgccg agcggtccgg cagggccttt tgcggccgcc tgctggagtg
    27061 ccccggtgag gttgaggccg gcctcgacgc acagggtgat gaggtcgagc acgaagggga
    27121 gctgcttgag catcagccgg cgtcggcagc ggatgcgatc gtccagccag gaccacgcga
    27181 tcagtgtgcc ggcggtggcg cccgccagcg cgggaagcgc cggaggaagt gcccatgcgt
    27241 gggcacaccc gaggccggct gccagcccgc acagcccgac ggcgacccgg tgtgcgagca
    27301 gctgcgccgg agagagcgtg tagtcgagtc cggcaaggcg cagtcgcacg gccagccgtg
    27361 cctgcagccg cgccggcagc agcggtgcga ccaggtgcgc gatccagcgc gtgggccacc
    27421 acagcaggcg caggaagcgc ggtggcgtgt cgagccaggt gcggtcctcg ggccggacgg
    27481 cgctgacgct gcgggccagc gcggcgacga tgccggcgct gccgagaccg gcgagcaggg
    27541 ccaccgccgc gatcaccgtg cgttcgtcga gcacaagcaa gtgcgcggag ctcatacgtc
    27601 gatcgccacg atgcgccgga tcagcagcac gccgagcacc tcgagcgtgg cgatcaccgc
    27661 cagcgtcgcc cagccgggcg gcgagtgcca caggaagccc atcgcctccg gttccatgcg
    27721 gtcgagcacc agcagcagcg ccagcggcag catgccgacc accaccgcct gaagcttgcc
    27781 ctgtgcggtg agggcgccga tcttgccttc catcgcgagc tggctgcgta cggtcagcgc
    27841 cgcgcgttcg agcgcttcgg ccaggctgcc gccggtctcg ttggcgatcc gcatcgcggc
    27901 gatcacgagg gtcagcgagg gcatgcgcac gcgtgcggcg agatggtcga gcgcctcgtc
    27961 gagcggaatg ccgaggcggt gctcgcgcag ggtgaggtcg aactcctggg cgatcggcgg
    28021 gcgcccttcg cggacgagtt gctgcagcgc caccgtcatc gaaacccccg cgcgcaggcc
    28081 gccggcgatc acctgcagcg cgtcgggcag ctgctgttcg atgaggtcga gccggcgccg
    28141 gcgcagcagt ccgaaggcaa gacgcggtag cagggccagc gcacccgcgc tggcgagtgc
    28201 gatcagcacc tcgccgctgg cgagccagct gccgatgccg ccgacggtga ccgcgaggag
    28261 gttcaggacg aagagtcgcg ccgggtccat gaacaggaag agttcttcga gctgcaggtg
    28321 ggcctcggcg gtgaagcgtt cgcggtagcg tgcgagcgca ttgcccgcga tgcgcattgc
    28381 cgaccaggcg agcagggcgg aggcgaggcc gaaggcgagc gtggccagca gcacggcggg
    28441 tgacgaagcc agggcattca tgaccgcttc cgatcgaaca ggtgcaggtc gagcgcaacc
    28501 ccttggcggc gcagttcgtc gtagaagccc ggcacggcat cgcagccggt gaagtggccg
    28561 ccgctcgggt cggcggtggg ggaatcggcg ctgcggcgct cgaagcggaa gagttcctgc
    28621 agctggatgc gtccgccctc catgccggtg agctcggtga tgctggtgag ccggcgcgcg
    28681 ccgcaggcga agcgggtctg ctggacgatg atgtccaccg cgctggcgat ctgctcgcgg
    28741 atcgcggcca gcgggagatc catgccggcc atcaggacga gcgtctcgag gcgggcgagc
    28801 gcatcgcgcg ggctgttcgc gtgcagggtc gtcatcgagc cctcgtggcc ggtgttcatc
    28861 gcctgcagca tgtcgagcgc ctcgccgccg cggcattcgc ccaccacgat gcggtcgggg
    28921 cgcatgcgca acgcgttgcg cacgaggtcg cggatgctga tcgcgccgcg cccttccagg
    28981 ttggacgggc gcgcctccag gctgaccagg tggctgtggc gcaggcgcag ctcggcggcg
    29041 tcctcgatgg tgatgatgcg ttcgccgtcg gggatgaagt tcgacagcag gttgaggaag
    29101 gtggtcttgc ccgagccggt gccgccggag accacgacgt tgcgccgctg ctcgacgcag
    29161 gtgcgcagga atgcggccat ctcgtgcgag agcgaaccca tgcggacgag gtccgcgacc
    29221 tcgagcgccc gacgcgcgaa cttgcggatg gtgagcgtcg ggcccttgag ggcgagcggc
    29281 gggatgatcg cattgacccg cgagccgtcg cgcagccggg catcgaccat cggcgaggat
    29341 tcgtcgatgc ggcggccgag cggcgcgacg atgcgctcga tgacgccgag caccgcgcgg
    29401 tcgctggtga aggtggtggg gtgcggcgcg atgcggccgc cacgttcgac gaagatctgg
    29461 tcgaagcgat tgaccatgat ctcgctcacc gactcgtcgg cgagcagggt ctcgagcggt
    29521 ccgaggccga tggcctcgtc gagcacctcg ctgcacagct gttcgcggtc gagcccgcct
    29581 ggaagcgcgg cctcggcggc gatgatctcg cgtacgagcg cggtggtctc ggcgcgcaac
    29641 tcgtcgtccg acatgcggtt gagatcgcgc cggcgcaggt cgatggtgtc gagcaggcgt
    29701 tcgtggatgc gtttgcgcca ggtgaaatcc gcgttcgctt cggcagcggg cgggtgcggc
    29761 tcgtcggccg ggcggactgg tggctcggat atgggcgcgg acgctgcggc cggcttatct
    29821 gttgctgcgg ccagggcgac ggacgagtct tcgggcggtt tgcgtgcggt gcggaaaaac
    29881 atgcaggcct ccggtcagtt gccgccgtag agggcgttcc acgcgggatc cgcgggctcg
    29941 cgcagcgccg gcgagggttc gggcgcaggg gcgagggcct gctcggcacg gcgttcgagc
    30001 agttgcgcgc gcggatccag aaggccttcg ccgggctcga gcagccaggg ggtgacgaag
    30061 accacgagtt cgctttcgcg gctgttgaac cgggtggacc ggaacagtcg accgattacc
    30121 gggagctcac ccaaatgcgg gagcgcgttc tcgtcgctct gctgttcgcg ctgcagcacc
    30181 ccggagagca ccagggtctc gccgttgcgc aggttgaact cggtctcggt tcggcgcgag
    30241 agcaggccgg gaacgccctg ggcggcgacg ctgcggtcga gcgagctgac ttcggtgcgg
    30301 atgcgcgcgc ggatcgttcc ggcggcgtcg gcgaccggtt cgaggatgtt gaggcggatg
    30361 ccgtattcct tgaacgtcac gctcggcgtc ccggtggcgg aggcgaccgg gacggggatt
    30421 tcaccgccgg cgaggaagct cgcttcgccg cggttgcgcg ccgagagtcg cggcgaggcg
    30481 aggatgaccg cgtcgccctt ctgcgcgagc aggttgaggc ggccgaagaa gctgccgacc
    30541 agggccacgt tggcggcgaa gggcgagacg cggttcgcct cgatctcgac ctcggagctc
    30601 agttcgccgc tgccgagcgt gcgcgtcacg gtcgccccgg tgcggttgtc gtggtacagg
    30661 tcgccgatca cgccgatccc gagcagcggc ttgccctgcg tggtccagtc gatgccgagg
    30721 tcgcgcaggc cgctgcggct gatctccacg aagcgcacgt cgaaggcgaa catgcggtcg
    30781 agcccgacgc ggctggtgaa gtcgatgagc tgcgggaaac gcttggcgag ctcggtgacc
    30841 ttgtcgcgtt cggcgtcgct caggccctcg ccctcgatga ccacgttgtc gccgatggtg
    30901 cgcgtgcgca gccgcgggac gtcgtgcagg aagccctgca ggtcgcgtgc gatgcgggcg
    30961 gtgtcggtct ggcggacggt gatcttgatg cgctggctgc ggcccgagcg ggtccacacc
    31021 tgcagcatgg tgtcgcccgg gccgttggcg atcaacagga tctcgcggtc gtcgagcacg
    31081 gccgcggaga gcaccttggc gtcgccgacg gcgaggcgct gcgcgtcgcg ctgcggcagc
    31141 acccgggttt cgccggcgaa catctggatc tcgtggacga ccgcgagcgc tgcgcgccca
    31201 ggttccggcg gcgcggcgct tgcgggcgga gcggcgcggc tcgggccggg caggcccagg
    31261 atcaggattg cgcacacgag gggagggagc gcccgccggc gcgccggcgg ggagatcggg
    31321 tcagtccggg tcatgggcag gctcggcgcg cggggtgggg cgggtaaggg gggacggggt
    31381 ggggacgagc gcggggttct cgatgccgcc gtagatgacc ggtacgcccg cttcgcgggc
    31441 tggcggtgcg aggccgagca gcgcgagcgc gtcggtccgc tgcagcggag ccgttcttgt
    31501 gtcccgagga tggcggagca ccgcggtcag gcgggcgagt gcgcgtgcgg cgacgagccg
    31561 ggtcgcctcc tcctcggaga gatccagggt cagggtgccg aagcttccac ccaggaggtc
    31621 gcccgcgcca tcgtcctcgc cgggccgggt gcggctcccg gtcgcgagca cgcgtacgcc
    31681 ctggagcagg ggcagggtca ggtgatggtg gtcgcgctgc acggtcgcga gcaggtcgat
    31741 gcggtcgccc gggcgcagca ggccggagag cgagctggtc tcgtcgaccg gaatcgtggt
    31801 ggcgcgccga cccggggcga gctgcgcgga gagggtggcg ggtcgccgct cttcgagctg
    31861 cgaccacacc agcggctcgc caccgcgagc cggctgggcg agcaccgcgc ctgccgcgcg
    31921 ctcgaactgc tccggggtga tcgcgctcga gtgcagccac tcgaccggca cttcgcgcac
    31981 ggcgacgagg tcgcgggtga ggggggtccc gaccgcgagg tccgtcttgg cgaccaccgc
    32041 atggcccatc tgcccttgct ggcgcagttc gatctcggtg gcgcgctcct ccaggtagcg
    32101 tgcggcgagc cacgcggcgc caaggccgaa gagggaggcg acggccagga tgatgaggtt
    32161 gcgggggact ctcttgagca tgggtggatc acaacggcag tgaaatgctg gcgagaaaac
    32221 gcgcgaagcc ggtggccacg gcttcggcga agaagacgag gagcggcttg ccgtcgaagg
    32281 ccggaaggcc gaagacgccg agcacgatgg cgaggatgac caggtattca tgcatcgcct
    32341 ggccgcggag gcgccggatc cgcttcatcg cccgctccgt gcgccgtggt cgatcatctg
    32401 cagcgtcacc gtggtcccgc tgccgtggcg gacgatggtc gcgaccgcct cgcggccccg
    32461 ggcgccgaac cacagcccca cgccctccag gccgggccgg gagaccggcg cacgacgctc
    32521 gagcgcgagg ccctgggccg agagctcgcg gaccaggcgc gccgcggccc aggagggcga
    32581 ggattcgtca tgccagacga ggatgcgtgc atgccggccg gcatcgttcg tttccaggtc
    32641 ggtgctgagc aggctgtccg ccgggcggcc cggagctggc gaggatggcg ccggatcgcg
    32701 cgagagatcg gactcggaca gggtgccttc ggtgcaggcg gccggcgccg tacggcatgg
    32761 acgcaggcgc aaggtgcgca aggccgcgcc gtgcttccag gacagcactt cccagccgtc
    32821 gatgcgcgag ctcaggggcg tgccctcgac cgctccctcg tagaaggcgc gcacgctggc
    32881 tgtcgcggtt cgcgcctcga accggcggat ccgggtcggt acgccatgga cgcgggcgtt
    32941 gtcgagcacc ggcgtgatac gggctccggc gaggggcggc aagggttcgg gcagggcggc
    33001 ctcggcgtgg gaaccgacca gcagcgcgaa ggcgatcgac aggcctgcgc gcgcgccgcg
    33061 accacgtccg tccggacgag gccggggatg gagggcagga gtcgtgtggc gcgaagtcgg
    33121 gaggcgcggg gcggggtgtg cgggcatggt cagcaccctt gttcgagacg gtcgcaaggc
    33181 aggatctcgg cgtgcacgcg cccgacctcg atcgccttgt cgagcaccag cggcggcagg
    33241 aggcgcccga ccgtattccc gatcagcgcc agcggccgga tcgggtaggc gagcgggccg
    33301 gcgtcttcga tgcggcgggc gtagtcggtc gatccggccg ccgaccagtt gtgaccgagc
    33361 aggacctggt ggcggcggat cgacagtccg agggtgtcga aggggggcag gccgggaacg
    33421 tcgcgcggcg acacgtggat cgtcccggtg tgttcggagc cggccggcag gtcgaggccg
    33481 tgcgggccgg cgagcagcgc ggaggccggt gccgcgttgg cgtgcgccgc gagatcgacc
    33541 ccgatccggc tggccgggtc ggcgagcagg tgttccccgc gatgatcgat ccacaagggg
    33601 ttggccggat gctccgtcgc ggacgcgttg ccggcaccgc tgaggatcgg cgctccgctt
    33661 gtggagaaga tgcgctcgcg gacctgcgcg gccagggctg cggccgtggg gggctgccgg
    33721 gcagggccca cgatcgaggt ctcgaaggcc acgtaacgtg ccgcctgctc ggtcgcgtgg
    33781 gcgaggtcga ggtacttgcc gagcagcggc aggctcagga agagcgggac caggaccagc
    33841 gcgagcacga ggaactcggt tgtcgactgg ccgccttgcg ctgcgcgcgc agccgggagg
    33901 agggggtggc agggcttcat ggcagcgcca tcccgtcctg ccgtccggcg gcccaggcgc
    33961 ggtccgtcgg ggtgacggcg gcgagcctgg cctgccagta ggggctgtac agacttgccc
    34021 gctcggtctt gttgtcgctg cgcgcggcgg gacgctcgaa gaacacctcg gcgcgcgcca
    34081 tcgcggcgag gtggccgccc ggcgcggtcg cttcgaatgc acgcaggttt ccctgcggtt
    34141 gcacgctgcc gatgcccccc gaaccgcgca gtctttcccg tgccttggca acgtggacgg
    34201 tcacgccgga gcggtgctct gcggcgggcg aaaggtcgcg cagctcgcgt gtctccggca
    34261 gcccgccgaa gtgcgcggcc cccgcatcga cgatcgcggg ctcggcccgg ttcagcgtcg
    34321 tgatcggatt gctcgtgtag gtgtcgccgt gatcgccacg ccgggcatcg gcaccctcat
    34381 cgcccgtaaa gccggcaccg ccccaggcga gcggcgtgcg acgccagcgc cagcgccagc
    34441 gtttcatccg ctggccctgg tgttcgagcg tgtcgagcgc gcgccattcg tcgtaaccga
    34501 tcagctcggt gccgccgcgt cgcttgagtt cgaccttgcg ttgcaatggc gggaggttca
    34561 agccgcggat cgaccagacg cgctcgcggg tgaatgcgtc gcgcgagcgt agcgtcacgt
    34621 cggcgatccg ttggcgcgca tcgccttgcg cggagtcctt cgccatcgtg cgggtgagat
    34681 tgcggaaccc gtggctgatc gggttgatcg acgctcgcat ttcgggatcg atccgacgca
    34741 cgaagtcgtt cgcgaaaccg gcctgggcga tgcccgtgtc caggctcgcg tgcatgacgc
    34801 gctgcgagag cgagagtgcg ttcgcgaccg cgtcgtgggc ggtggcgatg gtacccagcc
    34861 cgatatccag gccctccacg atcgcccgca gcgaaccgcc gctcccgaca tccagatagg
    34921 acgtgccatt gaccccggcg agcaattgaa ggaggcgcgg atactcgccg ggcttgtaca
    34981 gccagctcgc ggcgacctgg ttcaggttcg gagcgttttc gaccgccgtc tcgaagtagt
    35041 gcatccacga gatcaggctg atcgcctgcg cgatcgcgac ctcgttggcg acgatcgctc
    35101 gattggtgta ggcgtcgtag ttgagcgcgc gcgcctcgag tgtggccacg ctccaggcgg
    35161 tggcgtcggc ggcgttggtg aggcgcatct tttcgtcgac caccctgccg gtgttgaaca
    35221 tcagcagcag gaccgcgagg gtggcaaaga cgaggaagag accgaggacg agtgcctggc
    35281 cgtgctggcg gtcggctcgt tgcgggcgct gcgcgacgcc gggacgcggg gtggcggcca
    35341 tggcaaccgg tgcgggcggt tcggacaggc tcagcggccc gcgttgccgg tgtaggtgtc
    35401 gagcgtgcgg gcggtgttgg cctccgtcgc agccttgccg gccgcggttt gtgcggcgct
    35461 cttggcggtg ccgccgtcct tgccggcgat ctccgtggcg atcgccgcgg tctggttgcg
    35521 caccgtctgg ccgaagtact gatacacggc gatcgcggcg accgcgatca gggccacgat
    35581 gatgatgtac tcggtcatgc cctgaccgcg ctgccactgg atccttctgc ctgccgccat
    35641 gatcgatcct ccgggttgat gatgaaggga taatgacaaa cgggattggt gccgtcaata
    35701 tgcaaataag attctctgtt cgaggtcctc gggaggcgta aacgcaaaag cccgctgcag
    35761 ggcatgcagc gggcctccgg ggacagcgcg tccggtggat gtgtcagcgc gcgatcggct
    35821 tgtagcggat gcgcttgggc ttggcgcctt cctcgcccag gcgcttcttc ttgtcctctt
    35881 cgtattcctg gtagttgccc tcgaagaaga accactgcga gtcgccctcg gccgccagga
    35941 tgtgggtgca gatgcggtcg aggaaccagc ggtcgtgcga gatcaccagc gcgcagccgg
    36001 cgaactccag caatgcgtct tcgagcgcgc gcagggtctc gacgtcgagg tcgttggacg
    36061 gctcgtcgag cagcagcacg ttgccgccct ggatcagggt cttggcgagg tgcagccgcc
    36121 cgcgctcgcc gcccgacagg ttgccgacga tcttctgctg gtcgccgccc ttgaagttga
    36181 agcggccgat gtaggcgcgg ctgggcatct cgaagcggcc gaccgtgagc acgtcggcgc
    36241 catcggaaat ggcgtcgaac accgtcttgt cgttggccag gccctcgcgg gtctggtcga
    36301 ccgcggcgat cttgaccgtg gcgccgatct cgaccgtgcc ggagtcgggc ttgtcgcggc
    36361 cctcgatcat cttgaacagc gtcgatttgc cggcgccgtt ggggccgatg atgccgacga
    36421 tggcgccggg cgggatggcg aagctgacgt tgtccatcag cagcttgtcg ccgaaggcct
    36481 tgctgacgcc gtggaactcg atgaccttgt cgccgaggcg ctcgccgggc gggatgaaga
    36541 tctcctgggt ctcgttgcgg cgctggtatt cgacgctcgc catctcctcg tagcgggcca
    36601 ggcgggcctt cgacttggcc tggcgcgcct tggggttgga gcgcgcccac tccagctcgg
    36661 tcttcattgc cttctggtgg gcggcctcct gcttggcctc ctgcgccagg cggtcgccct
    36721 tctgctccag ccaggacgag tagttgccct tccaggggat gccgtggccg cggtcgagct
    36781 cgaggatcca ttcggcggcg ttgtcgagga agtagcggtc gtgggtgacg gccaccacgg
    36841 tgccggggaa gcgggtgagg aactgctcca gccactcgac cgattcggcg tcgaggtggt
    36901 tggtgggttc gtcgagcagc agcatgtcgg gcttggacag cagcagcttg cacagcgcga
    36961 cgcggcgctt ctcgccgccg gagagcttgc cgatcacggc gtcccagggc ggcaggcgca
    37021 gcgcgtcggc ggcgatctcc atctgggtct cgacgtcgct gccggcagtc gacaggatgt
    37081 tctcgtactt cgcctgttcc tcggcgagct tgtcgaagtc ggcgtcgggc tcggcatagg
    37141 cggcgtagac ctcttccagc ttctggcgcg cctccatgat ctcgccgagc gcagcctcga
    37201 cctcttcctt caccgtcttg gcggggtcga gctcgggctc ctgcggcagg tagccgatgc
    37261 gctggccggc gagccactgc acctcgccgt cgtattcctt gtccacgccg gccatgatgc
    37321 gcagcacggt ggacttgccc gagccgttga ggccgagcag gccgatcttg gcgccgggga
    37381 agaaggacag cgagatgt
//
LOCUS       DAOQBS010000016        35220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_641_length_35220_cov_15.437025, whole genome shotgun sequence.
ACCESSION   DAOQBS010000016 DAOQBS010000000
VERSION     DAOQBS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 35220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35220
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_641_length_35220_cov_15.437025"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..2328
                     /locus_tag="PKN25_03490"
     CDS             277..2328
                     /locus_tag="PKN25_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV89990.1"
                     /translation="MLRLRPLAVAVLGLSAFPVSSLVHAETLLGAQTVTAKGYAADTL
                     ETPQAVEILQAPAAGGTVAGALLRGKPGLAAQSDGAWGQNPVLRGLKKESVVVMVDGV
                     RMNSAQPQGAIASFLDLGLLERAEVVKGPGSVLYGSGAMGGVVNLITPDVGFSAEEAV
                     GGRVGARFSSVDKGVAGAALLTRSSAHGALVLGVAGRDVDDYRSPDGREDRTGYQSDT
                     LLAKYKHKLSEDLTLRVNLQRHEDEDVWYPGSARTGAGIPKPLGTATIHSPEQRRELY
                     ELGVDAKLGEGTLSAELYRQEVFRQIRAFSSAKQRDYVRNDVRFVTDGAKAKYLFPFG
                     ADHLLTVGADLWRMRGDPERYIDNNPPAFDNNLRNDPFDKGEIESAGVFVQDEFSLGD
                     TRIVAGARFDRVTGDAERKGMTQTTGLENADNNLSWSLGAIQPLSERLSAYANLGRAY
                     RAADMRERFEDSARGDGYFHVGNPQLDPEVSTSLELGLKRGGAGLRYQLAAFYTRIDD
                     YIAGRVTGAVHPQNGLPFKLTENLDKVTIYGIEGSASMPVGAFVADAGFTWLRGENHQ
                     DDEPLFQMPAPELTLGLGQAAERGFWWRAQLRAVAEQDRIATRFSNGTENPTAGFVTA
                     DAEFGWRFGKLGAFESAGLAVQLSNLADKRYHEHQTEGVSGNELAAPGRGVALSLNGS
                     F"
     gene            2330..3376
                     /locus_tag="PKN25_03495"
     CDS             2330..3376
                     /locus_tag="PKN25_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNV89991.1"
                     /translation="MGQDRRRPMAPPAWMRAVMHVCVVCAFLLVTGIVHAQGQKLAKL
                     TLAGPSAAVSNALIHMVDSGALADLAEVVEFVPWRDPDQLRVMALDGRADVLAMPTNV
                     AANLYNRGAKLQLINVSTWGVLWLVSRDPALKSLADLRGKELAMPFRGDMPDIVLQLL
                     AEGQGMDVRKDLNLRYVASPVDAMQLLVMRRVDHALLAEPAASMALRKTGSFPLSVIA
                     PELHRSVDLQKEWGRVFARAARIPQAGIAVMGALRERPELVARIEAEYARALAWCKVN
                     AAACGKAVAARIDVLSAEAVADSIAVSQLEVVPSRDARAELEHLFAKLVAKNPALVGG
                     KLPDDGFYGGAKAP"
     gene            3373..4164
                     /locus_tag="PKN25_03500"
     CDS             3373..4164
                     /locus_tag="PKN25_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNV89992.1"
                     /translation="MNLLRAWLAGLPAYLWSGWGAIASLCLLLAAWEAVSLAYGPLVM
                     PDPRAAFATLWGLIDSGAAWPELAATARRALTGYALSVVAGSVLGLVAGLSMTASMMA
                     RPLVTVLMGTPPIAWLVLAMLWFGASDGTPVFTVFVASFPVIFIGALQGTRTLDNQLH
                     GMAAAFRLPRRMMLTDVYLPHVVSYLFPSWIAALGTAWKVVVMAELLATQDGVGAALA
                     VSRSHLDTAATMAWIAAVVGLLLAVEYLVLEPIKREVERWREQGA"
     gene            4161..5075
                     /locus_tag="PKN25_03505"
     CDS             4161..5075
                     /locus_tag="PKN25_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV89993.1"
                     /translation="MNTISTAELRVHGLSHAFARDEVLSGIDFRVRAGEVVALVGPSG
                     CGKTTLLHLAAGLLTVQQGRVDSGFASTAFMFQQPRLLPWKSALDNVALGLKAAGVKR
                     AERHFRARALALRLGLAHRDLDKFPHQLSGGMQSRVALARALVLAPELLLLDEPFSAL
                     DVGLKEELYRLLLDHQAERGMGVLMITHDLMEAVRLSDAILVMAPAPGRIVCRFELDR
                     PAARRDDAWVYRHTAELLQVPDVRASFGLPPAVAVARDRAGSAPRPEFVRQGGLAAAA
                     RAEPLSGLRLASPTSRPIPVQGGKRCGA"
     gene            5114..6487
                     /locus_tag="PKN25_03510"
     CDS             5114..6487
                     /locus_tag="PKN25_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNV89994.1"
                     /translation="MTGKGAGNAQSGWSVRLAARPLFMCGFRPFFLLTALHAVLVLLA
                     WGGFLGFGLALPAVAGGPFVWHAHELMFGFGLAAVAGFVLTAVPEFTATPAFAPRVVL
                     RLVLLWLAARAAFWLSGGLAAVAESWLGGEEGQAVARWIGHLPAALLEIGFACALLAA
                     VAPRLWRDPERRHLGFQWGLAAMAMAVAGFHLDVLRGEYPMRWVLVGLGVMMTLVVVA
                     MSRISMRVMNDALDAARARRLPAHTLRRDEEAELELPSYRARPPRRNLAIAAIAVHTV
                     AEYLLPGSPITGWLGLGAAAAVFNLLNDWHVGRALFTRWAFLLYAVYWLLALGYLLLG
                     LARLGAPLAPSAGTHLLAIGAMGLSILAVLCIAGRTHAGYPLDERPWVGAAAVSIVLA
                     ALLRAAAGLPDMPASALQLLATLAWITAFALAAWRLGVLFVAARVDGGAGCEEYHAPR
                     AAEAVPP"
     gene            6509..7087
                     /locus_tag="PKN25_03515"
     CDS             6509..7087
                     /locus_tag="PKN25_03515"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNV89995.1"
                     /translation="MAPRSGRTDAGQRDRVRGSAGTERAKLAAVDGSAAESPSEPGDP
                     TMALLNWKADYCTGNAKIDEDHRALFERVNDFHFAWQQNRDRKDIARVLNRLVEYAEE
                     HFAREEEIMRASGYPGLARHQAIHEALFEQIFVLQGHFEDGRIRIDRDTVDFLRGWLV
                     DHIVGEDMKFAAYVESGDEPAPARQASDIDVV"
     gene            complement(7098..8300)
                     /locus_tag="PKN25_03520"
     CDS             complement(7098..8300)
                     /locus_tag="PKN25_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV89996.1"
                     /translation="MTGPHLLPLLLLYALMLPVTGMVPVLPEFTAQRFPGLGQFASHF
                     FMSINMIGALLGAPIAGLLSDRLGKRRLLAVGALAVNGIALLGIAWAWRSTESYALLL
                     ALRLVEGFAHMSALSLLMALAADHAGKAGLGARMGAVGASISLGVATGAPLGGIIGDI
                     DPFWVPLGGGLLSLAMAALGFVRLAEGGGTRPRMAASEIVDTLRNRRQLLIPLAFSFA
                     DRLTVGFIVSTLSLYLGLVIGFDARQIGIAMAAFLIPFSVLTWPAGHLSRHWDPLWMM
                     VIGSVLYGVFLAVLGFVPGDRVVATMAAGGVIAALMYAPSLVLAAQYGGSDCRASALA
                     AFNMAGSLGFAAGPLLSSALLAFFGLVLERPYPPVFVAIGLIEVVLALAVLLLVRRGR
                     LQAGAATA"
     gene            8478..8954
                     /locus_tag="PKN25_03525"
     CDS             8478..8954
                     /locus_tag="PKN25_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89997.1"
                     /translation="MRAVYLSGLLVVVLAVVTFWMTAGLQPGVTVLQFAWTPRGFGEI
                     VHVWSPEDLARYRLHLPVDSLLLLAYGSFGWLLATRTALFASLPGRARVAARFVLPLA
                     AVFDAVENALHAWLTEMPRFGMHGLYLLSTTCSALKWALLFGFAALVLWALAREAD"
     gene            complement(8960..9745)
                     /locus_tag="PKN25_03530"
     CDS             complement(8960..9745)
                     /locus_tag="PKN25_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HNV89998.1"
                     /translation="MSDATPVPLSPARLVSFRFHGELAAFLAPARRDRSFEVRCAAGS
                     SLKHVIEALGVPHTEVGRVLVDGRPAPLERRLCPGERVSVHPHAARCGKPCPSPPPRL
                     IADAHLGGLARLLRLAGFDTLYDNHIEDATLVATAVRERRVVLTRDRELLKRRDVAEG
                     CFVHALEPARQAVEILRRLGLAGHARPFSRCLECNAALHEVSPAAVVQRLPPAVRARH
                     THFSACAGCGRVYWEGSHWQRLHARIDALLAQARAAPQDEATD"
     gene            9844..10095
                     /locus_tag="PKN25_03535"
     CDS             9844..10095
                     /locus_tag="PKN25_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV89999.1"
                     /translation="MKIRALFVTLSGLSAAAVAVLVLAAVPAPAGAIAQPVGPAMPEE
                     CVCSRGLRLAEAPPSVLHNCQCGPLQCVVHAQSGQLQCR"
     gene            10109..10534
                     /locus_tag="PKN25_03540"
     CDS             10109..10534
                     /locus_tag="PKN25_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV90000.1"
                     /translation="MKPRLSVITLAVDDLERAVAFYRDGLGWPTQGIVGSAAQHSAVA
                     FFALQPGIRLALFPRTSLAVEAGVAEPPSGTSGCLLSHNVARREQVAEVMAEAAVAGA
                     RILRPPRDTAWGGHAGYFADLDGHPWEVAWNPKWPLADD"
     assembly_gap    10807..10906
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10928..11884
                     /locus_tag="PKN25_03545"
     CDS             10928..11884
                     /locus_tag="PKN25_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV90001.1"
                     /translation="MVVETHTVGVRALADDVGAVGSLVSNESVVLRPEVSGRIEAIRF
                     RDGEAVRRGEVLIEFDAAVQRAELQQARASLTLAESNFRRSQDLFSRSFVSRSSLDNA
                     RSQLEVARAGVALAQARLARMQIRAPFAGVVGIRSVSPGDFVKDGEAMINLEDIATLK
                     VDFRLPELYLDRVRPGQTVEIASDVLAGERFAATVEAIDPLVDAQGRALRLRASLPNP
                     ELRLRPGVFVRVRLILAERADVVMVPEAALVPAPGNTQFVYRVAEGKAQRVEVRTGTR
                     RDAMVEVVEGVQAGEVVVTAGQLKLRDGAAVKLATGGVARAD"
     gene            11888..14959
                     /locus_tag="PKN25_03550"
     CDS             11888..14959
                     /locus_tag="PKN25_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV90002.1"
                     /translation="MVLSELCIRRPVFATVLSLLVLLVGLMSYSRLTVREYPNIDEPV
                     VTVDTRYRGASAEIVESQVTKPLEDSLAGIEGVDVLSSISRQERSQITVRFRVERSAD
                     SAAADVRDRVSRVRQRLPDDIDEPVIAKVEADASPIIWVAFSSDRHAPMEISDFANRV
                     IKPRLQTLPGAADARLFGDRRSAMRVWLDRDRLAAFGLTVQEVEDAIRRQNVELPAGR
                     IESREREFAVVAQTDLARPEQFGAIVLRQGDTADAYPVRIRDVGRVEVAPESERTSVR
                     FMGRPAVSIGLIKQSVANPLDLKRGLDEMMPRLQAELPEGMSMTIANDTTVFIERSID
                     AVFSTIWEAVLLVAAVCLFFLRNWRAMLVPLVTIPVSLVGAFTLMLVLGFSINTLTLL
                     ALVLAIGLVVDDAIVVLENIYRHIEDGMEPVAAAFKGAREIGFAVVAMTITLAAVYAP
                     VAFMSGRTGKLFTEFALTLAGAVIVSGFVALTLSPMMCSKLLRHEPTHGRIYNGIERF
                     LAWLTGGYRAVLELALGARWLVMLVFAVVAGSSVWLMGQLKSELAPLEDRGRVIGMFT
                     GPEGATLDYMARYAEEIERIYAGLPEVDRYLVIAGNPTVSQGISFVGFTDWSERTRTA
                     GEISSGLQPKLRAVPGVNAFPIMPASFGQSARSRPVSFVVATSESYDALARVTDTFLD
                     AMRDNPGFLSPDTDLKLTMPELAVEVDRDRAADLGVDVDVIARTLESMLGGRQVTRYK
                     QDAEQYDVIVQVGAGERRDPRDIRDIYVRARTGEMVPLTSVVQVSERVAPRELNHFGQ
                     RRSVTISANLAPDYALGEALQWMDDTAARILPPGYSTDYDGQSREFKTSSESLALVFL
                     LALAFIYLVLAAQFESFRDPFIIMLTVPLSMTGALGALWLTGGTLNVYSQIGLVTLVG
                     LITKHGILIVEFANQLREHGHALREAVVEAAVLRLRPILMTTGAMVLGSLPLALATGA
                     GAESRQQIGWVIVGGLLIGTFFTLFVVPTVYTLLARREREAPVAVAQGAAS"
     gene            complement(15021..15242)
                     /locus_tag="PKN25_03555"
     CDS             complement(15021..15242)
                     /locus_tag="PKN25_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HNV90003.1"
                     /translation="MFPEYRDLITRLKHNDRHFTQLFERHNELDQKIHNIETHVVPGT
                     SLEIEVMKKEKLAIKDQIYAYLKRVETES"
     gene            complement(15457..16002)
                     /locus_tag="PKN25_03560"
     CDS             complement(15457..16002)
                     /locus_tag="PKN25_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004378041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HNV90004.1"
                     /translation="MDEQIIADVEQWLDEVVIGLDLCPFAARPRREKRVRIAVSHATT
                     ADALLEDLQAELERLSDTPVAELETTLLAIPDMLEDFADYNDFLDAVDLWVEQFGWEG
                     ELQVASFHPQYQFADTEADDPGNLTNRSPWPLLHIIREESLEKAIEHYPEVDRIPERN
                     IARMNALSGEERAKLFPHLFG"
     gene            complement(16017..17000)
                     /gene="miaA"
                     /locus_tag="PKN25_03565"
     CDS             complement(16017..17000)
                     /gene="miaA"
                     /locus_tag="PKN25_03565"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNV90005.1"
                     /translation="MPPPIPAPLPPALLLLGPTASGKTASALALAQALPVEVISVDSA
                     LVYRDMDIGTAKPNAEERAVCPHHLIDVVTPEESYSAARFRADAIRLMGEIVARGNIP
                     LLAGGTMLYFKALRDGLSDLPAADPELRRALDDEAAGRGWPALHAELARLDPEAAARL
                     EPGDAQRIQRALEIVRLTGRPLADSYAKKEDDPLPCRLLPIALAPSDRSALHARIEER
                     FDAMLHAGLVDEVRQLRARYRLDPSMPSMRCVGYRQVWEYLDGGTGYDELRFKGVAAT
                     RQLAKRQLTWQRQFRETWPGFVELDCLRPDLPEAVLHTALRLLHDLPLHPA"
     gene            complement(17214..17777)
                     /locus_tag="PKN25_03570"
     CDS             complement(17214..17777)
                     /locus_tag="PKN25_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNV90006.1"
                     /translation="MRKPAILAAALAAAVLVTAGCATEPVASRKDISFDAAAANPLIP
                     SNYAAADSLLAQLRGQLAPTQALIAATVVNIDALEQSSTLGRLISEQVSARFTLAGYR
                     MIEMKFRNNVYMARDQGELMLTREIRDLASTHDAQAVVVGTYAQSSEMVFVNLKVIQP
                     ETNVVLAVHDYALPLDPMTRSMLRGHR"
     gene            complement(17777..18292)
                     /locus_tag="PKN25_03575"
     CDS             complement(17777..18292)
                     /locus_tag="PKN25_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90007.1"
                     /translation="MKKTLAAASAAALLAGACATPVHHAPAANAQAAVSQRALHSASH
                     WNSLARDVAQRLSARLAPQTALFVNQHADASAFDRAFSTQLITALLEAGHPVLRTPAG
                     ALRVGVDTQVKPGVDATGSLEIIVSTSVTDNSRFIARETGVFRADAADEALYLAAPQA
                     RTKSFQVVGGE"
     gene            18682..19263
                     /locus_tag="PKN25_03580"
     CDS             18682..19263
                     /locus_tag="PKN25_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNV90008.1"
                     /translation="MKIFSPLYERTMVWSRHPRAPWFLGGLSFAESSFFPIPPDVMLA
                     PMCLANPHRAWWFALLTTLTSVAGGLFGYLIGYYAFDLIEPWLRGSNYWGAYEQAVAW
                     FDAWGFWAIFIAGFSPIPYKVFTIAAGVASMALLPFTLASLIGRGARFFLVAGLMAWG
                     GERMEGALRKYVDRIGWATVAAVVVGVLVYRAG"
     gene            complement(19306..21192)
                     /gene="mutL"
                     /locus_tag="PKN25_03585"
     CDS             complement(19306..21192)
                     /gene="mutL"
                     /locus_tag="PKN25_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNV90009.1"
                     /translation="MPHIHRLSDLLVNQIAAGEVVERPASVLKEVLENAVDAGARAIE
                     VQLEQGGVRRIRVADDGCGIARDELALALERHATSKIATLDDLERVGTMGFRGEALAA
                     IAAVARTGITSRAEGASHAWRIEAGREPEPAALDQGTVVDVADLYYNTPARRKFLKTE
                     STEFAHCDDMFRRVALARPDIGLQLAHNGRVIHRLPPSPPAARVAALMGDDFLQHARE
                     VQADAGILRLAGFASLPAYSRASRDAQYFFVNGRFVRDKLLAHAVREAYADILHGARH
                     PAYVLFLELDPAGVDVNVHPAKIEVRFRESRAVHQFVFHAVRRTLAESGAGRAAELHA
                     PAAIGVGAGPAASPASGTVPQGGFSSALPPLSVPTAAHHPASRSWPPADARQGRLAME
                     SASRAYFDFAAGARDSGGASLPDRPGEIQSGPRPAPTETATGEAPPLGYALGQLHGIY
                     ILAQNAAGLVLVDMHAAHERILYEKLKTVIDGRPAVQRLLIPAVFSVGAKDMAAAEEG
                     TEVLAGMGFEIAAAGPQELVVRSVPALLASAPVAELVRELLQELREFPATEVVTARRN
                     ELLATMACHGAVRANRQLTLPEMNALLRDMEATERADQCNHGRPTWTQLSLAELDRFF
                     MRGQ"
     gene            21407..22063
                     /gene="purN"
                     /locus_tag="PKN25_03590"
     CDS             21407..22063
                     /gene="purN"
                     /locus_tag="PKN25_03590"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNV90010.1"
                     /translation="MKSIVILISGRGSNMEAIVRAGIPGARIAAVISNRPGAGGLEFA
                     RAHGIATAVVDHKSHPDRAGFDQALAECIDAHAPDLVVLAGFMRVLGDGFVRRYEGRL
                     LNIHPSLLPAFPGLHTHRRALETGVKVHGASVHFVTAELDDGPIVIQAAVPVLTGDDE
                     DKLAARVLAQEHLIYPQAVRWFVEDRLELVAGRVSLRDAPAQDGEALLVPAPEGGGRA
                     "
     gene            22063..22800
                     /locus_tag="PKN25_03595"
     CDS             22063..22800
                     /locus_tag="PKN25_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNV90011.1"
                     /translation="MRGLVVRLALAACASAAALPVGAAAEPPAQGHIVYDVHYGSSGF
                     KVGEARHEWKLDKGRYALSLALQAKGLAGLFGLEYEQRSSGTVDAKGMRPELFEVDQR
                     GRKPERAEFDWTAGKVSIRRGGEERRNGEIRAGDQDLLSLWHQARKVVESGKPARFAV
                     ITNKSIKQAVVEPRGEERLELAGEQLDTVRLHAFAEKDDLDIDIWLSRRHSLLPVRIR
                     ITDDKGGVLDQRAARIEAGVPAARNPN"
     gene            22802..24145
                     /locus_tag="PKN25_03600"
     CDS             22802..24145
                     /locus_tag="PKN25_03600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNV90012.1"
                     /translation="MKPKTEKPRTRAIKRQEPAAAERPAEGAVSRTLFIQATQALGAV
                     LAFDYPADAVLSRHFRDNRELGHRDRGFIAEAVYGVLRRLRWLRRIAGAEATPRMLLL
                     AWFARGEGWPMRNFEGLASATERDWITSIKAAEAGEPSLAERADLPDWLAERLLAQMD
                     GDELLALAHGLNRPAPLDLRVNLIKSDRDALLERLQADGVRAEPGRLSPQAIRLAGKP
                     ALQKHPLFLDGSFEVQDEGSQLLGLLVQPRRGELVVDFCAGAGGKTLQLGAMMRSTGR
                     LYAFDVSEKRLAKLKPRMARAGLSNVHPVLIAHEADAKVKRLAGKADRVLVDAPCTGL
                     GTLRRNPDLKWRQSPAAVEEMVAKQGAILAAAARVVRPGGRLVYATCSLLAEENDGIV
                     DAFLAAHPEFRALSAQEVLAAQGVALETGERLRLLPHVHDTDGFFAAVMERAKGG"
     gene            24195..24749
                     /locus_tag="PKN25_03605"
     CDS             24195..24749
                     /locus_tag="PKN25_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HNV90013.1"
                     /translation="MLGWMAALALLALPGVHASERFAGKGEIELAFAPSDDTEKVVIN
                     LIRGARKSLEVHAYAFTSRNIADAMVAAQRRGVRVEVLADAQMNRREKGNAIPRLLAA
                     DVPVAFETRYNAAHNKVLIVDAEGPRCAVLTGSYNFTWSADNRNAENILIVRDHCELA
                     RAYRANWLRHRKDATRVTSLPWRE"
     gene            24899..26068
                     /locus_tag="PKN25_03610"
     CDS             24899..26068
                     /locus_tag="PKN25_03610"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNV90014.1"
                     /translation="MYNGLFDLPWWGYVLVALALTHVTIVSVTVFLHRHQAHRALELH
                     ALPSHFFRFWLWLTTGMVTKEWAAIHRKHHAKCETAEDPHSPQTRGLHKVLWEGAELY
                     RAEAKNVETLQRYGHGTPDDWLERKVYRHSILGVAIMLVIDLVAFGPLGLTIWAVQMA
                     WIPFWAAGVVNGVGHFWGYRNYDCDDASRNLVPWGILIGGEELHNNHHSFATSAKLSA
                     KWYEFDIGWMYIRLLELLGLAKVKKTIPRPRFGEVKPVPDLDTLQAIVTHRYDVMTRY
                     VRSLREVFVDEARRLGEVHGLELSARRLRRWVLGGELRGLDDTQRRRLEQAVEGSPAL
                     RTVAAMRDELAALWSRSNASREQLLAQLQDWVARAERSGIRPLQEFSLRLRSYAA"
     gene            complement(26095..26538)
                     /gene="queD"
                     /locus_tag="PKN25_03615"
     CDS             complement(26095..26538)
                     /gene="queD"
                     /locus_tag="PKN25_03615"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNV90015.1"
                     /translation="MRITRRLEFDAGHRIPDHASQCRHLHGHRYALEVTLSGDIIKAD
                     GVPVNGMVMDFADVKRIANEYVVGPWDHAFLAYRGDHLVLDLLAAIPGHKTVVLDVVP
                     TAENLAAEAFRILDPAYRDTYGNHLRLERVRLYETPNCWADALRD"
     gene            26626..27504
                     /locus_tag="PKN25_03620"
     CDS             26626..27504
                     /locus_tag="PKN25_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV90016.1"
                     /translation="MNSSQGQPPGTLATLTVGKHDRDLAGLTYVYPVLSRRAGGVSVG
                     INLNPNNACNWHCAYCQVPDLVRGKAPEIDLARLESELRGFLADLIHGSWMERHVPEG
                     ARVIRDIAFSGNGEATSAEVFPEAVALVLRLRDEFGLSPQAVPVRLITNGSLLAQARV
                     QEGVRRLGEAGGEVWFKVDGGSVEAIERINGVRLEPAAVARNLTRCAELCPTWVQTCM
                     LRWDGALPSDADIDAWLAVLEAAGTERLRGVLLYGVARPSMQPEAPRIATLAAEELEA
                     IAERARKKGLTVRVSP"
     gene            complement(27522..27815)
                     /locus_tag="PKN25_03625"
     CDS             complement(27522..27815)
                     /locus_tag="PKN25_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV90017.1"
                     /translation="MKANEKLDVREIRRKLGLNQSQFWSKIGVTQSGGSRYESGRNIP
                     RPVQALLRLVHVEQIDINKVKRDDVEVAEYLKATNPELYKTLKKEARAKRKER"
     gene            28070..29302
                     /locus_tag="PKN25_03630"
     CDS             28070..29302
                     /locus_tag="PKN25_03630"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNV90018.1"
                     /translation="MALIVQKYGGTSVGTPERMRSVARRVANFRAQGHRVVVVVSAMS
                     GETNRLIALARAVAPSPRTRELDVVVSTGEQVSIGLLCMALDDLGVPARSYTGAQVRI
                     LTDSTHTRARILDIDAAPIRKDLDAGGVVVVAGFQGVDAHGNITTLGRGGSDTTAVAL
                     AAALEADECQIYTDVDGVYTADPRIVPEARKLDAVRFEEMLELASLGSKVLQIRAVEF
                     AAKYKVKLRVLSSFEEDPGEGTFITVEESSNMERPVISGIAFVRDEAKLTLTGVPDRP
                     GIAYQILGPIADADIDVDMIIQNAPRDGSTDLSFTVARADLARAVKVLEGVQAHVGAR
                     SIESDDTMCKVSVVGVGMRSHPGVAAKMFRTLAEEGINIQMITSSEIRISVAIEEKHL
                     ERAVRALHKAYGLDVAAE"
     gene            29363..29456
                     /locus_tag="PKN25_03635"
     tRNA            29363..29456
                     /locus_tag="PKN25_03635"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29397..29399,aa:Ser,seq:gct)
     gene            29579..29875
                     /locus_tag="PKN25_03640"
     CDS             29579..29875
                     /locus_tag="PKN25_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HNV90019.1"
                     /translation="MRHDLKVPFAEKDQAKKLGARWDAARKRWYIDGGLDPAVFARWQ
                     PMPHDTAAAASRRGAPAGVEGRVQVGSRFVALPRVCDCLPWDDCDVCRLQAWPQ"
     gene            29891..30502
                     /locus_tag="PKN25_03645"
     CDS             29891..30502
                     /locus_tag="PKN25_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNV90020.1"
                     /translation="MEAVAVIDFETTGLSPAQGDRATEIAAVIVRDGRVVDRYQSLMN
                     AGVRIPAHIESLTGISNAMIRSAPTAAQVMREVADFIGGIPMVAHNAAFDAKFWDAEL
                     ARIERARKQDFVCSLLLSRRLFPLAPSHRLGALVEYARLPVAGRFHRALADAEMAASL
                     LLRLEEELRQRHRVAQVSVELLCRIQRASKAQLPKCLAAAREG"
     gene            complement(30587..31093)
                     /locus_tag="PKN25_03650"
     CDS             complement(30587..31093)
                     /locus_tag="PKN25_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNV90021.1"
                     /translation="MNSKDTTPPASTFSTPIDDRYFEDYVPGAVHRFGEMRVTAQEII
                     EFAHRYDPQDFHTDPAKAADTQFGGLIASGWMTCGMMMRMYSEHYLTHNASLASPGID
                     ELRWLEPVRPDDVLSTRVTIVEARRSASKPDRGLVRSKIEVLNQHDRVVLSMLAMNLI
                     GCRTPAAA"
     gene            31279..32538
                     /locus_tag="PKN25_03655"
     CDS             31279..32538
                     /locus_tag="PKN25_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNV90022.1"
                     /translation="MTQPPRACGIDFGTSNSTVGWLRPDAPILVPLEDGRATLPSAVF
                     FNADEDSIQFGRAALAEYLEGYEGRLMRALKSLLGTSLIDGQTEVNGRALGFRELLAS
                     FIGELKARAERATGRTFDHAVFGRPVHFVDEDETADRKAQDTLEAIARQVGFREIGFQ
                     FEPIGAALHYERTLSREELVLIADIGGGTADFSIVRLSPQRARAVERREDLLGNAGIH
                     IGGTDFDRVLSLDCVMPVLGYRSRLRNGATMPASVYFQLAAWHTINFAYTRQVWADLQ
                     QVYRDALARTELDRLCRLINLREGHWLAMQVEEAKIALSAAERTQVVLDRIEPGLVLE
                     LSRAAFTAATQGLVERVGGCVAGLLAASGVDRERIDTLYFTGGASGVPQLRARIAAEL
                     PRARCVEGDLYGSIGAGLAVEAARRYG"
     gene            complement(32529..33725)
                     /locus_tag="PKN25_03660"
     CDS             complement(32529..33725)
                     /locus_tag="PKN25_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNV90023.1"
                     /translation="MNHRERFLELQALLASHDALWRPPAFQAGLPPWCFERPALAAEL
                     RALDEATLEQLVQEPDEALRWLAPHLPALLRSVELCALPALPARALPEPDRRFDAGVP
                     GRKRAQIEAFAARLPATGLPLLEWCAGKGHLGRRLALADGVPVRSLEIDPALCADAER
                     LAERSRLPQRVVCADALDPASRPHLHGHAVLALHACGELHRSLVRSADRDDAAAYAIA
                     PCCYHRGANGGYRALGRDASLELDAATLRLAVTETVTAPRHDRRRLARDQAWKLGFIA
                     LRQTAEGGPVPHFRPVPPAWLNGSFEDFCRRLAEREGLRLPPALDWAEWEAVGERRRD
                     EVRRLELVRHAFRRALEVWLALDLVLGFEARGFTARIGTFCERALTPRNLLILASRRG
                     EGPLQP"
     gene            complement(33869..34801)
                     /locus_tag="PKN25_03665"
     CDS             complement(33869..34801)
                     /locus_tag="PKN25_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNV90024.1"
                     /translation="MNKQLRNAVLVALGALAAGTAGAKEGGDQYPNGIETWFAGAVPP
                     PGNYFINYFGHYGGDLYNGEGKRVAGTSASAWFNAFRFVKVSGIKVLGGDWAGHVILP
                     VVHQRLNLGGGSASASGLGDLTIDPFIVAWHQGDWHWTVGLDINLPIGRYKSGDPRTS
                     IGANYWSLEPIFAFTWLSQSGWEVSAKLMYNIKGGNDDFRASPGAAKQKYDSGDDFHM
                     DYLVGRRFGPWGVGLSGYYLKQTTDDKVDGTVIPAIPGVWSRGRRGEVLAAGPSLTYS
                     TKGGTMLIAQWQHEFERENRFGGDKAVLKLVMPF"
     gene            complement(34890..>35220)
                     /locus_tag="PKN25_03670"
     CDS             complement(34890..>35220)
                     /locus_tag="PKN25_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNV90025.1"
                     /translation="IYHEETFAPVKAIVRVQGTEAAIACANDNPFGLAAAVFGRDLAR
                     AWQVAGRIQSGICHINGPTVHDEAQMPFGGVKDSGWGRFGGQAGIEAFTELRWITLQT
                     SPRHYPF"
BASE COUNT         4954 a        11811 c        12937 g         5418 t
ORIGIN      
        1 gagagcaggt tgagcacgat catcagccag aagcggcggt cttgcgggcg gtagaggcga
       61 gagagcttca tcggcggggg cgggagagat cgaggggagg gtttgcgtac gcatgatagc
      121 ggcggctggc gaaaagccgg gtcgcgcggg cggaatttga taaaacgcaa gtgcgaacaa
      181 ttcgcattac gatagtatcg ctccctgatc ttcctgttcg ggcatgccag ccgtcgccag
      241 ccagcccggg aaccccactt cctggagctt tccatcgtgc ttcgtctgcg cccgctcgcg
      301 gtggccgtcc tcggcctgtc cgccttcccc gtttccagcc tcgtccatgc cgagaccctg
      361 ctcggcgccc agaccgtcac cgccaagggc tacgccgccg acaccctgga gaccccgcag
      421 gcggtcgaga tcctgcaggc gccggcggcc ggcggcacgg tcgcgggcgc gctgctgcgc
      481 ggcaagcccg gcctggcggc gcagtcggac ggtgcctggg ggcagaaccc ggtgctgcgc
      541 gggctcaaga aggaaagcgt ggtggtgatg gtcgacggcg tgcgcatgaa ctcggcgcag
      601 ccgcagggcg cgatcgcctc cttcctcgac ctcggcctgc tcgagcgcgc cgaggtggtc
      661 aaggggccgg gctcggtgct gtacggcagc ggcgcgatgg gcggggtggt caacctgatc
      721 acgcccgatg tcggcttcag cgccgaagag gcggtgggcg ggcgcgtcgg cgcgcgcttc
      781 tccagcgtgg acaagggcgt cgccggtgcc gcgctgctga cgcgctcgag cgcgcacggc
      841 gcgctggtgc tgggcgtggc cgggcgtgac gtggacgact accgcagccc ggacggccgc
      901 gaggatcgca ccggttacca gtccgacacg ctgctggcaa agtacaagca caagctgagc
      961 gaggacctga ccctgcgggt gaacctgcag cggcatgagg acgaggatgt gtggtatccg
     1021 ggatcggcgc gcaccggcgc cggaatcccc aagccgctcg gcaccgcgac catccattct
     1081 cccgagcagc gccgcgagct ctacgagctt ggcgtggacg cgaagctggg cgagggcacg
     1141 ctgtcggccg agctctaccg tcaggaagtg ttccgccaga tccgcgcgtt ctcgtcggcc
     1201 aagcagcgcg actatgtgcg taacgacgtc cgcttcgtca ccgatggcgc gaaggcgaag
     1261 tacctgttcc cgttcggcgc cgaccacctg ctgaccgtgg gtgccgacct ctggcgcatg
     1321 cgcggcgatc cggagcgcta catcgacaac aatccgccgg ccttcgacaa caacctgcgc
     1381 aacgatcctt tcgacaaggg cgagatcgag tcggcgggcg tcttcgtgca ggatgagttc
     1441 agtctcggcg atacccggat cgtcgctggg gcgcgtttcg atcgcgtcac cggtgacgct
     1501 gaacgcaagg gcatgacgca gaccaccggg ctggagaacg ccgacaacaa cctgtcgtgg
     1561 tcgctcggtg cgatccagcc gctgagcgag cgcctgagcg cctacgccaa cctcggccgc
     1621 gcctaccgcg cggcggacat gcgagagcgc ttcgaggact cggcgcgcgg cgacggctac
     1681 ttccatgtcg gcaacccgca gctcgacccg gaggtgtcga ccagtctcga actcggcctg
     1741 aagcggggcg gcgccggcct gcggtaccag cttgccgcct tctatacgcg catcgacgac
     1801 tacatcgccg gccgtgtcac cggggccgtg catccgcaga acggcctgcc cttcaagctc
     1861 accgagaacc tcgacaaggt cacgatctac ggcatcgagg gcagcgccag catgccggtc
     1921 ggcgccttcg tcgccgacgc gggcttcacc tggctgcgcg gcgagaacca tcaggacgac
     1981 gagccgctgt tccagatgcc tgcgcccgag ctgaccctgg ggcttggcca ggcggccgaa
     2041 cgtggcttct ggtggcgcgc gcaattgcgc gcggtcgccg agcaggaccg catcgcgacc
     2101 cgcttctcca acggcaccga aaaccccacc gccggcttcg tcaccgccga cgccgagttc
     2161 ggctggcgct tcggcaagct cggcgccttc gaatccgccg ggctggccgt gcagctgagc
     2221 aacctcgccg acaagcgcta tcacgagcac cagaccgagg gcgtgtccgg caacgaactc
     2281 gccgcgcccg gccgtggcgt ggcgctcagc ctgaacggga gcttctgaca tgggccagga
     2341 tcgtcgccgc ccgatggcgc ccccggcgtg gatgcgcgcg gtcatgcacg tctgcgtggt
     2401 gtgcgccttc ctgctcgtga ccggcatcgt ccatgcgcag gggcagaagc ttgccaaact
     2461 gaccctggcc ggtccgtcgg ccgcggtgtc gaacgcgctc atccacatgg tcgactcggg
     2521 tgcgctcgcc gacctcgccg aggtggtcga gttcgtgccg tggcgcgacc ccgaccagtt
     2581 gcgcgtgatg gcgctcgacg ggcgcgccga cgtgctggcg atgccgacca acgtcgccgc
     2641 caacctctac aaccgcggcg cgaaactgca gctgatcaac gtgtcgacct ggggtgtgct
     2701 gtggctcgtg tcgcgcgatc cggcgctgaa gtcgctggcc gacctgcggg gcaaggagct
     2761 ggcgatgccg tttcgcggcg acatgcccga catcgtgctg caactccttg ccgaagggca
     2821 gggcatggac gtgaggaagg acctgaacct gcgctacgtc gccagcccgg tggacgcgat
     2881 gcagctgctg gtgatgcgcc gcgtcgacca cgcgctgctc gccgagccgg cggcctcgat
     2941 ggcgctgcgc aagaccgggt ccttcccgct cagcgtgatc gcgcccgagc tgcaccgcag
     3001 cgtggacctg cagaaggagt ggggccgggt gttcgcgcgt gcggcacgca tcccgcaggc
     3061 gggcatcgcg gtcatgggcg cgctgcgcga gcgccccgag ctggtggcgc gcatcgaggc
     3121 cgagtacgcg cgcgcgctcg cgtggtgcaa ggtcaacgcc gcggcctgcg gcaaggcggt
     3181 ggcggcgcgc atcgacgtgc tcagcgccga ggcggtggcc gattcgatcg cggtgagcca
     3241 gctcgaggtg gtgccgagcc gcgatgcgcg tgccgagctc gagcacctgt tcgccaagct
     3301 ggtggcgaag aatcccgcac tggtcggcgg caagctgccg gacgatggct tctacggcgg
     3361 agcgaaggcg ccatgaacct gctgcgcgcc tggctggccg gcttgccggc ctacctgtgg
     3421 agtggctggg gcgcgatcgc cagcctctgt ctgctgctcg ccgcctggga ggcggtgagt
     3481 ctcgcctacg gcccgctcgt catgcccgac ccgcgcgcgg ccttcgccac gctgtggggc
     3541 ctgatcgaca gtggcgcggc gtggccggaa ctggccgcca ccgcacgccg cgcgcttacc
     3601 ggctacgcgc tgtcggtggt cgccggcagc gtgctcggcc tggtcgccgg gctgtcgatg
     3661 accgcctcga tgatggcgcg tccgctcgtc accgtgctca tgggcacgcc gccgatcgcc
     3721 tggctggtgc tggcgatgct gtggttcggc gcgagtgatg gaacacccgt gttcaccgtg
     3781 ttcgtggcgt ccttcccggt gatcttcatc ggcgcactgc agggcacgcg cacgctcgac
     3841 aaccagctcc acggcatggc cgcggccttc cgcctgccac gccgcatgat gctcaccgac
     3901 gtgtacctgc cgcacgtggt gtcctacctg ttcccgtcct ggatcgccgc gctcggcacg
     3961 gcctggaagg tggtggtgat ggccgagctg ctcgccaccc aggacggtgt gggcgcggcg
     4021 ctcgcggtgt cgcgctcgca cctggacacc gccgcgacca tggcctggat cgccgcggtg
     4081 gtcggcctgc tgctggcggt ggagtacctg gtgctggagc cgatcaagcg tgaggtcgaa
     4141 cgctggcggg agcagggcgc atgaacacga tttcgactgc cgagctgcgc gtgcacgggc
     4201 tctcccatgc cttcgcgcgc gacgaggtgc tctctggcat cgacttccgc gtgcgtgcgg
     4261 gcgaggtcgt ggccctggtc ggaccctcgg gctgcggcaa gaccacgctg ctgcacctgg
     4321 cggccggcct gctgacggtg cagcaaggcc gggtggacag cggcttcgcc tccaccgcct
     4381 tcatgttcca gcagccgcgc ctgctgccct ggaagagcgc gctcgacaac gtcgcgctcg
     4441 gcctcaaggc ggccggcgtc aagcgcgccg agcgccactt ccgcgcgcgc gcgctggcct
     4501 tgcgcctcgg actcgcccac cgcgacctcg acaagttccc ccaccagctc tcgggcggca
     4561 tgcagagccg ggtggcgctg gcgcgcgcgc tggtgctcgc gcccgagctg ctgctgctcg
     4621 acgagccgtt ctcggcactg gacgtcggcc tcaaggaaga gctttatcgc ctgctgctcg
     4681 accaccaggc cgagcgcggc atgggcgtgc tgatgatcac ccacgacctc atggaggcgg
     4741 tgcggctgtc ggatgcgatc ctggtgatgg cgcccgcgcc cgggcgcatc gtgtgccgct
     4801 tcgagctcga ccgcccggcg gcccggcgcg atgacgcctg ggtgtaccgg cacaccgcag
     4861 agctgctgca ggtgccggac gtacgcgcga gcttcggctt gccaccggcg gtggcggtgg
     4921 cgagggatcg agcgggatct gcgccgcggc cagagttcgt gcggcaaggc gggctggccg
     4981 ccgccgcacg cgctgagccg ctgtccgggc tgcgcctggc gagcccgact tccagaccga
     5041 tcccggtgca gggcggcaag cgttgcgggg catgagcatg ggtgacagga cgatgaatgc
     5101 tggacgcggg gccgtgaccg ggaagggggc gggcaatgcg caatcgggct ggagcgtgcg
     5161 gcttgcggca cggccactgt tcatgtgcgg cttccggccc ttcttcctcc tcaccgcgct
     5221 ccatgcggtg ctggtgctgc tcgcctgggg cggcttcctc ggcttcggcc tggcgctgcc
     5281 ggcggtcgcg ggcggcccct tcgtgtggca cgcgcacgag ctgatgttcg gcttcgggct
     5341 cgccgcggtg gcgggcttcg tgctcaccgc ggtaccggag ttcaccgcca cgccggcgtt
     5401 cgcgccgcgg gttgtgctgc gtctggtact gctgtggctg gcggcgcgcg cggccttctg
     5461 gctgtcgggc gggctcgccg ccgtcgcgga gagctggctc ggcggggagg aggggcaggc
     5521 cgttgcgcga tggatcgggc acctgcccgc ggccctgctc gagatcggct tcgcctgcgc
     5581 attgctggcc gcggtggcgc cgcggctgtg gcgcgacccc gagcgtcgcc acctcggctt
     5641 tcagtggggc ctggcggcga tggcgatggc ggtggcgggc ttccacctcg atgtgctgcg
     5701 cggcgagtac ccgatgcgct gggtgctggt ggggctgggc gtgatgatga cgctggtggt
     5761 ggtggcgatg agccggatct cgatgcgcgt catgaacgac gcgctcgacg ccgcgcgcgc
     5821 gcgccgcctg cccgcgcata cgttgcgccg ggacgaggag gccgagctgg aactgccttc
     5881 ctaccgcgcg cggccgccgc ggcgcaacct ggcgatcgcg gcgatcgcgg tccatacggt
     5941 ggccgaatac ctcctgccgg gctcgccgat caccggttgg ctggggctgg gcgcggcggc
     6001 ggcggtgttc aacctgctca acgactggca cgtcgggcgc gccttgttca cgcgctgggc
     6061 cttcctgctc tatgcggtgt actggctgct ggcgctcggc tacctgctgc tcggcctcgc
     6121 ccgtctgggg gcgccgctgg caccgagcgc cgggactcac ctgctggcga tcggcgcgat
     6181 gggcctgtcc atcctggcgg tgctctgcat cgccgggcgc acgcacgccg gctacccgct
     6241 cgacgagcgt ccgtgggtgg gggcggcggc agtctcgatc gtgctcgcgg ccttgctgcg
     6301 cgcggccgcc ggcctgcccg acatgcccgc atccgccttg cagttgcttg ccacgctggc
     6361 ctggatcaca gccttcgcgc tggccgcgtg gcggttgggc gtgttgttcg tggccgcgcg
     6421 ggtggatggc ggtgccgggt gcgaggaata ccacgctccc cgggcagccg aggccgtccc
     6481 gccctgatgc gggcacaggc ctgcggtgat ggcgccgcgc tcgggtcgga ccgacgcagg
     6541 ccagcgtgac cgggttcgtg gcagtgctgg caccgaacgc gctaagctgg cggccgtgga
     6601 tggctccgca gcggaatcgc cgtccgagcc cggagacccg acgatggcgc tgctcaactg
     6661 gaaagccgac tattgcaccg gtaacgcgaa gatcgacgag gatcaccgcg ccttgttcga
     6721 gcgcgtcaac gattttcact ttgcgtggca acagaaccgc gaccgcaagg acatcgcgcg
     6781 cgtgctcaac cgcctggtcg agtacgccga ggaacacttc gcgcgcgagg aagagatcat
     6841 gcgcgccagc ggctacccgg gactggcgcg ccaccaggcg atccacgagg cactgttcga
     6901 gcagatcttc gtgctgcagg gccacttcga ggacgggcgc atccgcatcg atcgcgacac
     6961 ggtcgacttc ctgcgcggct ggctggtgga tcacatcgtc ggcgaggaca tgaagtttgc
     7021 ggcctatgtc gagagcggcg acgaacctgc gccggcccgg caggcgagcg acatcgacgt
     7081 cgtctgaacc cgggccctca ggccgtcgcg gcgccggcct gcagccggcc gcggcgcacc
     7141 agcagcagca ccgcgagggc gagcacgacc tcgatcagcc cgatcgccac gaagaccggc
     7201 gggtaggggc gttccagcac caggccgaag aaggcgagca gcgcgctgct gagcagcggg
     7261 ccggcggcaa agccgagcga gcccgccatg ttgaaggcgg ccagcgcgct ggcgcggcag
     7321 tcgctgccgc catattgcgc ggccagcacc agcgagggcg catacatcag cgccgcgatc
     7381 acgccgcccg cggccatcgt cgccaccacc cgatcgcccg ggacgaagcc gagcacggcg
     7441 aggaagacgc cgtagagcac gctgccgatc accatcatcc acaacggatc ccaatgccgc
     7501 gacaggtggc cggcgggcca ggtgagcacc gagaagggga tcaggaaggc cgccatcgcg
     7561 atgccgatct ggcgcgcatc gaaaccgatc accaggccga ggtagagcga cagcgtcgac
     7621 acgatgaagc ctacggtgag gcggtcggcg aaggagaacg cgagcgggat cagcaactgg
     7681 cgacggttgc gcagggtgtc gacgatctcg gatgccgcca tgcgcggccg cgtgccgccg
     7741 ccctcggcga ggcggacgaa gccgagcgcg gccatcgcca gcgagagcag gccgccgccg
     7801 aggggcaccc agaaggggtc gatgtcgccg atgatgcccc cgagcggcgc gccggtggcc
     7861 acacccaggc tgatcgaggc gcccaccgcg cccatgcgcg cgcccagccc ggccttgccg
     7921 gcgtggtcgg cggcgagcgc catcagcagc gacagcgccg acatgtgggc gaagccctcg
     7981 acgaggcgca gcgcgaggag cagcgcgtag ctctccgtgc tgcgccaggc ccaggcgatg
     8041 cccagcagcg cgatgccgtt cacggcgagc gcgcctaccg cgagcaggcg gcgcttgccg
     8101 aggcggtcgg agagcaggcc ggcgatcggc gcgccgagca gcgcgccgat catgttgatc
     8161 gacatgaaga agtggctggc gaactggccc agaccgggaa agcgctgcgc ggtgaactcg
     8221 ggcagcaccg gcaccatgcc ggtgaccggc agcatcaggg cgtagagcag cagcagcggc
     8281 aggagatgag gtccggtcat ggtgtcggcg cggtgtcggc gcgctgggtt cggaggcggg
     8341 tgcgccgcat gatacttcga cctccgcgac cgtcgtctgt tgccgatcaa ggcccggcta
     8401 caatcctggc gacccgccgc cgccgcggcc gggttcgtgt cggcaccccg tgtgcgtctc
     8461 acttcggagg ttcgcgcatg cgcgccgtgt atctgtcggg actgctggtg gttgtgctcg
     8521 ccgtcgtcac cttctggatg accgcggggc ttcagccggg cgtgaccgtg ctgcagttcg
     8581 cgtggacgcc gcgcggcttc ggcgagatcg tccatgtgtg gtcgcccgag gatcttgccc
     8641 gctaccgcct ccaccttccg gtcgacagcc tgctgctgct ggcctacggc agcttcggct
     8701 ggctgctcgc gacccgcacg gcgctgttcg cgtcgctgcc cgggcgggca cgcgttgcgg
     8761 cacgcttcgt cctgccgctc gcggccgtgt tcgacgcggt ggagaatgcg ctccacgcct
     8821 ggttgaccga gatgccgcgc ttcggcatgc atgggctgta cctgctgtcg accacctgct
     8881 cggcgctcaa gtgggcgctg ctgttcggct tcgccgcgct ggtgttgtgg gcgctggcgc
     8941 gcgaagccga ctgaggccct cagtccgtgg cctcgtcctg cggcgccgcg cgcgcctgcg
     9001 cgagcagggc gtcgatgcgc gcgtgcaggc gctgccagtg cgagccttcc caatacaccc
     9061 ggccgcagcc ggcgcaggcg gaaaagtgtg tgtgacgcgc gcgcaccgcg ggcggcaggc
     9121 gctgcaccac cgccgctggc gagacttcgt gcagtgccgc gttgcattcg aggcagcgcg
     9181 agaacgggcg ggcgtgtccg gcgagtccga ggcggcggag gatctcgacc gcctggcgcg
     9241 ccggctcgag cgcatgcacg aagcagccct cggcgacgtc ccggcgcttg agcagttcgc
     9301 ggtcgcgggt cagcacgacg cgccgctcgc gcacggcggt ggcgacgagc gtggcgtcct
     9361 cgatgtgatt gtcgtagagc gtgtcgaagc cggccaggcg cagcagtcgc gccaggccgc
     9421 cgagatgggc gtcggcgatg aggcgcggcg gcggggacgg gcagggcttc ccgcagcgcg
     9481 cggcatgggg atggaccgac acccgctcgc ccgggcacaa gcggcgctcg aggggcgccg
     9541 ggcgaccgtc gaccagcacc cttccgacct cggtgtgcgg gacgccgagc gcttcgatca
     9601 cgtgcttgag gctggagccg gccgcgcagc ggacctcgaa gctgcggtcg cgccgtgccg
     9661 gggcgaggaa ggccgccaac tcgccatgga agcggaagga gacgagccgt gcgggggaga
     9721 ggggcacggg ggtggcgtcg gacatgggca gggcattccc tcgatcgagc accgccacag
     9781 catagcgcgc gccgcaggcg cttgctgcta cgctgtcggc tggttcgtcc gaggagatcg
     9841 aagatgaaga tccgcgccct gttcgtcacg ctttccgggc tctccgccgc cgcggtggcc
     9901 gtactcgtcc tcgccgcggt cccggcaccc gccggcgcga tcgcccagcc ggtgggcccg
     9961 gcgatgccgg aggaatgcgt ctgttcgcgc ggcctgcgcc tcgcggaggc gccgccctcg
    10021 gtcctgcaca actgccagtg cggtccgctg cagtgcgtgg tgcacgcgca gtccggccag
    10081 ttgcagtgcc gctgagccgc gtgtgcgcat gaagccgcgc ctcagcgtga tcacgctcgc
    10141 ggtggacgat ctcgagcgcg ccgtcgcctt ctatcgcgac ggcttgggct ggccgacgca
    10201 gggcatcgtg gggagtgcgg cgcagcattc ggcggtcgcc ttcttcgcgc tgcagcccgg
    10261 gatccggctg gcgctcttcc cgcgcacgag tctcgcggtc gaggccggcg tcgcggaacc
    10321 gccgtcgggc acgagcggct gcctgctgag ccacaacgtc gcccggcgcg agcaggtcgc
    10381 cgaggtgatg gccgaggccg cggtggcggg tgcgcgcatt ctgcgtccgc cacgcgacac
    10441 cgcctggggt ggccatgcgg gctatttcgc ggatctcgac gggcatccgt gggaggtggc
    10501 gtggaacccg aaatggccgc tggcggacga ctgagtcccg cgcaggctgg ctgagcggat
    10561 gcagcccggc gcaacgcgac cgtcccgcgg cgcggaaccg gacagcgcgc gcctttgtcc
    10621 ctaaactggc ggttcgcgcc gagccacgct ctccactcct cacgcccgac tcctcgtttc
    10681 ccgatgcctt ccagccgccg cttcgtcctc tcgctcagcc tcgtcggtct cgccgcgctg
    10741 ggcgcctatg cctggtatgc aaaccgtccg ccggccttgc cgccgggggg cgcgcgggcg
    10801 caggcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggtg gctcgcaggg
    10921 cgggccggtg gtggtggaga cgcacaccgt gggcgtgcgt gcgctcgccg acgacgtcgg
    10981 cgcggtcggc tcgctggtgt cgaacgagtc ggtggtgctg cgtcccgagg tgtcggggcg
    11041 gatcgaggcg atccgctttc gcgatggcga ggcggtgcgg cgcggcgagg tgctgatcga
    11101 gttcgacgcc gcggtgcagc gcgccgagct gcagcaggcg cgcgccagcc tgaccttggc
    11161 cgaatcgaac ttccgccgca gccaggacct cttctcgcgc agcttcgtga gccgcagcag
    11221 cctggacaac gcgcgctcgc agctcgaggt ggcgcgcgcc ggggtggcgc tcgcgcaggc
    11281 caggctcgcg cgcatgcaga tccgcgcgcc gtttgccggc gtcgtgggca tccgcagcgt
    11341 gagccccggc gacttcgtca aggacggcga ggccatgatc aacctcgagg acatcgccac
    11401 gctgaaggtg gatttccgcc tgcccgagct ctacctcgac cgcgtgcgcc ccgggcagac
    11461 ggtggagatc gcgagcgacg tgctggcggg cgagcgtttc gccgccacgg tcgaggcgat
    11521 cgatccgctg gtcgatgcgc aggggcgcgc gctcaggctg cgcgcgagcc tgcccaaccc
    11581 cgagctgcgc ctgcgcccgg gcgtgttcgt acgcgtgcgc ctgatcctcg ccgagcgcgc
    11641 cgacgtcgtg atggtgcccg aggccgcgct ggtgccggcg ccgggcaaca cccagttcgt
    11701 ataccgggtc gccgagggca aggcgcagcg cgtcgaggtg cgcaccggca cgcggcgcga
    11761 cgcgatggtg gaggtggtcg agggcgtgca ggcgggcgag gtggtggtga ccgccggcca
    11821 gctcaagctg cgcgacggcg cggcggtgaa gctggccacg ggcggcgtgg cgcgggcgga
    11881 ctgagcgatg gtcctctccg agctctgcat ccgccgcccg gtgttcgcca ccgtgctgtc
    11941 gctgctcgtg ctgctggtgg gcctgatgtc gtactcgcgg ctcaccgtgc gcgagtaccc
    12001 caacatcgac gagccggtcg tgacggtgga tacgcgctac cgtggcgcca gcgccgagat
    12061 cgtcgagtcg caggtcacca agccgctgga ggactcgctc gccggcatcg agggcgtgga
    12121 cgtgctgtcc tcgatcagcc gccaggagcg cagccagatc acggtgcgct tccgcgtcga
    12181 gcgcagcgcc gattcggcgg cggccgacgt gcgcgaccgc gtgtcgcgcg tgcgccagcg
    12241 cctgcccgac gacatcgacg agccggtgat cgccaaggtc gaggccgacg ccagcccgat
    12301 catctgggtg gctttttcct ccgaccgcca cgcgccgatg gagatcagcg acttcgccaa
    12361 ccgcgtcatc aagccgcgtc tgcagacctt gcccggtgcg gccgatgcgc gcctgttcgg
    12421 cgaccgccgc tcggcgatgc gggtgtggct cgatcgcgac cgcctcgccg ccttcggcct
    12481 gaccgtgcag gaggtggaag acgcgatccg gcgccagaac gtggaactgc ccgccgggcg
    12541 catcgagagc cgcgagcgcg agttcgccgt cgtggcgcag accgacctcg cccggcccga
    12601 gcagttcggc gccatcgtgc tgcgccaggg cgacaccgcc gacgcctacc cggtgcgcat
    12661 ccgcgacgtc ggccgggtgg aggtggcacc cgagagcgag cgcacctcgg tgcgcttcat
    12721 ggggcggccg gcggtgtcga tcggcctcat caagcaatcg gtggcgaacc cgctcgacct
    12781 caagcgtggc ctggacgaga tgatgccgcg gctgcaggcc gagctgcccg aaggcatgtc
    12841 gatgaccatc gccaacgaca ccacggtgtt catcgagcgc tcgatcgacg cggtgttctc
    12901 caccatctgg gaggcggtgc tgctggtggc cgcggtgtgc ctgttcttcc tgcgcaactg
    12961 gcgggcgatg ctggtgccgc tggtgacgat cccggtgtcg ctggtgggcg cgttcacgct
    13021 gatgctggtg ctcggcttct cgatcaacac gctcacgctg ctcgcgctgg tgctggcgat
    13081 cgggctggtg gtggacgatg cgatcgtggt gctggagaac atctaccgcc acatcgagga
    13141 cggcatggag ccggtcgccg ccgccttcaa gggcgccagg gagatcggct tcgcggtggt
    13201 ggcgatgacg atcacgctgg ccgcggtgta cgcgccggtg gccttcatga gcgggcgcac
    13261 gggcaagctc ttcaccgagt tcgcgctcac gctcgccggc gcggtgatcg tgtcgggctt
    13321 cgtcgcgctg acgctgtcgc cgatgatgtg ctcgaagctg ctgcgtcacg agcccacgca
    13381 cggacggatc tacaacggca tcgagcgttt cctggcctgg ctcaccggcg gttatcgcgc
    13441 tgtgctcgag ctggcgctgg gcgcgcgctg gctggtgatg ctggtgttcg cggtggtggc
    13501 ggggagctcg gtctggctga tggggcagtt gaagagcgag ctcgcgccgc tggaggaccg
    13561 cggtcgggtg atcggcatgt tcaccggccc ggagggcgcc acgctcgact acatggcgcg
    13621 ttacgccgag gagatcgagc gcatctacgc cggcctgccg gaagtcgatc gctatctggt
    13681 gatcgccggc aacccgacgg tgtcgcaggg catctccttc gtcggcttca ccgactggtc
    13741 ggagcgtacg cgtacggccg gggagatctc gagcggactg cagcccaagc tgcgcgccgt
    13801 gccgggcgtg aatgccttcc cgatcatgcc ggcttcgttc gggcagagtg cgcgctcgcg
    13861 gccggtgagc ttcgtggtgg cgacctcgga gagctacgac gcgctcgcgc gcgtgaccga
    13921 caccttcctc gacgcgatgc gcgacaaccc cggcttcctg tcgccggaca ccgacctcaa
    13981 gctgacgatg cccgagcttg cggtggaggt ggatcgcgac cgcgcggccg acctcggggt
    14041 cgacgtcgat gtgatcgcgc gcacgctcga gagcatgctc ggaggtcgcc aggtcacgcg
    14101 ctacaagcag gacgccgagc agtacgacgt gatcgtgcag gtcggcgccg gcgagcgccg
    14161 cgacccgcgc gacatccgcg acatctacgt gcgtgcgcgc accggcgaga tggtgccgct
    14221 gaccagcgtg gtgcaggtga gcgagcgcgt ggcgccgcgc gaactcaacc acttcggcca
    14281 gcgccgctcg gtgaccatct cggccaacct ggcgcccgat tacgccctgg gcgaggcgct
    14341 gcagtggatg gacgacaccg cggcgcgcat cctgccgccg ggctacagca ccgactacga
    14401 cggccagtcg cgcgaattca agaccagctc ggagagcctg gcgctggtgt tcctgctcgc
    14461 gctcgccttc atctacctgg tgctggcggc gcagttcgag agcttccgcg accccttcat
    14521 catcatgctg acggtgccgc tgtcgatgac cggcgcgctc ggcgcgctat ggctgaccgg
    14581 cggcacgctc aacgtctaca gccagatcgg cctggtgacg ctggtcgggc tgatcaccaa
    14641 gcacggcatc ctgatcgtgg agttcgccaa ccagctgcgc gagcacggac atgcgctgcg
    14701 cgaggcggtg gtggaggcgg cggtgctgcg cctgcggccg atcctgatga cgaccggggc
    14761 gatggtgctg ggctcgctgc cgcttgcgct ggcgaccggg gcgggcgccg agagccgcca
    14821 gcagatcggc tgggtgatcg tggggggctt gctgatcggc accttcttca cgctcttcgt
    14881 ggtgccgacg gtgtatacgc tgctggcgcg gcgcgagcgc gaagcgccgg tcgcggtcgc
    14941 gcagggagcc gcctcctgaa aacgaagaac cccgccgcgc cggtcgcgct tgcggggttc
    15001 gtcacggtgc cgggagggga tcagctttcg gtttcgaccc tcttcaggta cgcgtagatc
    15061 tggtccttga tcgccagctt ttccttcttc atgacctcga tctcgaggct ggtgccgggc
    15121 acgacgtggg tctcgatgtt gtggatcttc tggtcgagct cgttgtgcct ctcgaagagc
    15181 tgggtgaagt ggcggtcgtt gtgcttgaga cgggtgatca ggtcgcgata ttcggggaac
    15241 atgggaagaa ctccttggtt ctggattgca aaggctacga actggggcaa aggaaaaact
    15301 ttccgctcgg ccccagtcta ctcccgcttc cggtgcgcgg ccagcgtggc gggcgggtgt
    15361 ggcccgtggt ccgcgacccg cttcgggccg cttgcggatg gcatttgccg ggctgttgcg
    15421 gcgagcagga aaactgatcg aaagttcctc ggtgcctcat ccgaacaggt gggggaacag
    15481 cttcgcgcgc tcctcgccac tgagtgcgtt catgcgcgcg atgttgcgct cggggatgcg
    15541 gtcgacctcg gggtaatgct cgatcgcctt ctccaggctc tcctcgcgga tgatgtgcag
    15601 cagcggccag ggcgagcggt tggtcaggtt gcccgggtcg tccgcctcgg tgtcggcgaa
    15661 ctggtactgc gggtggaagc tcgccacctg cagctcgccc tcccagccga actgctcgac
    15721 ccacaggtcc accgcgtcga ggaagtcgtt gtagtcggcg aagtcctcca gcatgtcggg
    15781 gatcgccagc agggtggtct cgagctccgc caccggggtg tcggagaggc gctcgagctc
    15841 ggcctgcagg tcttcgagca gggcgtcggc ggtggtggcg tggctgaccg cgatgcgcac
    15901 gcgcttctcg cgccgcgggc gggcggcgaa ggggcagagg tcgaggccga tgacgacctc
    15961 gtcgagccac tgctcgacgt cggcgatgat ctgttcatcc attggagcgg ttccgttcag
    16021 gcgggatgca ggggaaggtc gtgcagcagc cgcaaggcgg tgtgcagcac ggcctcgggg
    16081 aggtcggggc gcaggcagtc gagctcgacg aagcccggcc aggtctcgcg gaactggcgc
    16141 tgccaggtga gctggcgctt ggcgagctgg cgggtggcgg cgacgccctt gaagcgcagc
    16201 tcgtcgtagc cggtgccgcc gtcgaggtat tcccacacct ggcggtagcc gacgcagcgc
    16261 atcgaaggca tggacgggtc gagccggtag cgcgcgcgca gctggcgaac ctcgtcgacc
    16321 aggccggcgt ggagcatggc gtcgaagcgc tcctcgatgc gcgcgtgcag cgcgctacgg
    16381 tccgacggtg cgagcgcgat cggcagcagc cggcaaggca gcgggtcgtc ttccttcttc
    16441 gcgtagctgt ccgcgagagg gcggccggta aggcggacga tctcgagcgc gcgctggatg
    16501 cgctgcgcgt cgcccggctc caggcgtgcc gcggcctcgg ggtcgaggcg ggcgagctcg
    16561 gcgtgcagcg ccggccagcc gcgcccggcg gcctcgtcgt cgagcgcgcg gcgcagctcg
    16621 gggtcggccg cgggcaggtc ggagagcccg tcgcgcagcg ccttgaagta cagcatggtg
    16681 ccgccggcca ggagcggaat attgccgcgt gcgacgatct cgcccatcag gcggatcgcg
    16741 tcggcgcgga agcgtgcggc cgaatagctc tcctcgggcg tcaccacgtc gatcaggtgg
    16801 tgcgggcaca ccgcgcgttc ctccgcgttc ggcttggcgg tgccgatgtc catgtcgcga
    16861 tacaccagcg ccgaatccac gctgatcacc tccaccggca gggcttgcgc gagcgcaagc
    16921 gcgctcgcgg tcttgccgct ggcggtgggg ccgagcagca gcagggcggg cggcaggggg
    16981 gcggggatgg gcggaggcag tttgggcgag gccgaagtgt gcgcgcgggc gcgggattcg
    17041 cgacggcgca gggcgcgcgg attatctccc aggccgtcgt gatccgccag cgcgcgaccc
    17101 gcaacccctc aggtttgccg agaacggcgg cgcatttcca ggctgatggg gccggtgccg
    17161 aaaagcttgc ggtcggcggg ggcgcgcgtt cgcccgcccc cgggatgcag cgatcagcgg
    17221 tggccgcgca gcatcgagcg cgtcatcggg tcgagcggca gggcgtagtc gtgcaccgcc
    17281 agcaccacgt tggtctcggg ctggatgacc ttgaggttca cgaacaccat ctcgctgctc
    17341 tgcgcgtagg tgcccaccac caccgcctgc gcgtcgtgcg tgctggcgag gtcgcggatc
    17401 tcgcgggtga gcatcagctc gccctggtcg cgcgccatat agacgttgtt gcggaacttc
    17461 atctcgatca tgcggtagcc ggcgagcgtg aagcgcgccg agacctgttc ggagatgagg
    17521 cggccgaggg tggaggattg ctcgagcgcg tcgatgttca ccacggtggc ggcgatcagt
    17581 gcctgcgtcg gcgcgagctg gccgcgcagc tgcgcgagca ggctgtcggc cgcggcgtag
    17641 ttggacggga tcagcgggtt ggcggcggcg gcgtcgaagc tgatgtcctt gcgcgacgcg
    17701 accggttcgg tggcgcagcc ggcggtgacg agcacggcgg cggcgagggc tgcggcgagg
    17761 attgcgggct tgcgcatcat tcgcctccga cgacctggaa ggacttggtg cgcgcctgcg
    17821 gggcggcgag gtagagcgcc tcgtcggccg cgtccgcgcg gaacacgccg gtctcgcgcg
    17881 cgatgaaccg gctgttgtcc gtcacgctgg tgctgacgat gatctcgagc gagccggtgg
    17941 catcgacgcc gggcttgacc tgggtatcca cgccgacgcg cagggcgccg gccggggtgc
    18001 gcagcaccgg gtggccggcc tcgagcaggg cggtgatcaa ctgcgtggaa aaggcgcggt
    18061 cgaaggccga agcgtcggca tgctgattga cgaacagcgc ggtctgcggg gccaggcggg
    18121 cggacaggcg ctgcgcgacg tcgcgggcga ggctgttcca gtgcgaggcg gagtgcagtg
    18181 cgcgctgcga aacagctgcc tgggcgttcg ccgcgggggc gtgatggacg ggcgtggcgc
    18241 aggcgcctgc cagcagggcc gcagcgctgg ccgcggcgag ggtcttcttc atggagcgca
    18301 ttctccggga cggggcaccg cgtcggtcgc aggcggcgag gacggtgccg ggcaagtgaa
    18361 acggcgtatg tcgcagctat gccgaaagct agcagaagcc ccgctccgac tcaattgcgc
    18421 cgcatcggcg gatgagacat agttccgcgc cgtggcgctg cacaaaaaac gggcagcggg
    18481 ggaatacgcc tgtccgtcag cgccttgcgg aacttatcca cagcttgtcc cgggagctgc
    18541 ccacgggtgc tggggacaaa gccggcggtc gccatggtga gcgatgcgtg gatgagcgtg
    18601 cccgcaaagg agaagtggct tgcaggtatg attgcgcgcg ctgtgcacgg ggcgtccgtg
    18661 caatcctgtc gaggtgcttt cttgaagatc ttttcgccgc tgtacgagcg cacgatggtg
    18721 tggtcgcgtc atccgcgcgc gccctggttc ctgggggggc tgagtttcgc cgagtcgtcg
    18781 ttctttccga tcccgccgga cgtcatgctc gcgccgatgt gcctggcgaa tccgcatcgc
    18841 gcctggtggt tcgcgctcct gaccaccctg acctcggtgg cggggggact tttcggctac
    18901 ctcatcggct actacgcctt cgacctcatc gagccctggc tgcgcggcag caactactgg
    18961 ggcgcctacg agcaggcggt ggcatggttc gatgcctggg gcttctgggc gatcttcatc
    19021 gccggctttt cgccgattcc ctacaaggtg ttcacgatcg ccgcaggcgt ggcgtcgatg
    19081 gccctgctgc cattcacgct ggcctcgttg atcgggcgcg gcgcgcgctt cttcctcgtc
    19141 gcgggcctga tggcctgggg gggcgagcgc atggagggtg cgctgcgcaa gtatgtggac
    19201 cgcatcggct gggccacggt ggccgcggtg gtggtcgggg tgctggtgta tcgcgcggga
    19261 tgatgcaggc gcgccgacgg gcgcgcattc ggctccgggg gtgcgctact gcccgcgcat
    19321 gaagaagcgg tcgagctcgg ccaggctcag ctgcgtccac gtggggcggc cgtggttgca
    19381 ctggtcggcg cgctcggtcg cctccatgtc gcgcagcagg gcgttcatct cgggcagggt
    19441 gagctggcga ttggcgcgca ccgcgccgtg gcaggccatg gtggcgagca gctcgttgcg
    19501 gcgcgcggtg acgacctcgg tggcggggaa ttcgcgcagc tcctgcagca gctcgcgcac
    19561 cagttcggcc accggcgcgc tcgccagcag cgcgggcacg ctgcgcacca cgagctcctg
    19621 cggaccggcg gcggcgatct cgaagcccat gccggcgagc acctcggtgc cttcctcggc
    19681 cgccgccatg tccttcgcgc ccaccgagaa caccgccggg atcagcaggc gctgcacggc
    19741 cgggcggccg tcgatcacgg tcttgagctt ctcgtagagg atgcgttcgt gggcggcgtg
    19801 catgtccacc agcacgaggc cggcggcatt ctgggcgagg atgtagatgc cgtgcagctg
    19861 gccgagcgcg tagccgagcg gcggcgcctc gcccgtggcg gtctccgtcg gcgccgggcg
    19921 cgggcccgac tggatctcgc cggggcggtc gggcaggctg gcgccaccgc tatcgcgtgc
    19981 gcccgcggcg aaatcaaagt aggcgcggct cgccgactcc atcgccaggc gtccttggcg
    20041 ggcgtctgcg ggcggccagg accgcgatgc ggggtggtgg gcggccgtcg ggacggaaag
    20101 cggggggagg gcgctcgaga agcccccttg cgggacggtg cccgatgccg gggaggccgc
    20161 ggggccggca ccgacgccaa tggctgcggg cgcgtgcagc tccgcagcgc gtcccgctcc
    20221 gctctcggcc agggtgcggc gcacggcgtg gaagacgaac tgatgcaccg cgcgcgactc
    20281 gcggaagcgc acctcgatct tggccggatg cacgttcaca tccaccccgg cggggtcgag
    20341 ctcgaggaag agcacgtaag ccggatggcg cgcgccgtgc aggatgtcgg cgtaggcttc
    20401 gcgcaccgcg tgggcgagca gcttgtcgcg cacgaagcgg ccattgacga agaaatactg
    20461 cgcgtcgcgg ctggcgcgcg aataggcggg cagcgacgcg aagccggcca ggcgcagtat
    20521 cccggcgtcg gcctggacct cgcgggcgtg ctgcaggaag tcgtcgccca tcagcgccgc
    20581 cacccgcgcg gcgggcggtg agggagggag gcggtggatc acgcggccgt tgtgggcgag
    20641 ctgcaggccg atgtcggggc gcgcgagcgc gacgcggcgg aacatgtcgt cgcagtgggc
    20701 gaactcggtg gattcggtct tgaggaactt ccgccgcgcg ggggtgttgt agtagaggtc
    20761 ggcgacgtcg accactgtgc cctggtcgag cgccgccggc tcgggctcgc gcccggcctc
    20821 gatgcgccag gcgtggctcg cgccctcggc gcggctggtg atgccggtgc gcgccaccgc
    20881 ggcgatggcc gccagcgcct cgccgcgaaa ccccatcgtg cccacgcgct cgaggtcgtc
    20941 gagggtggcg atcttgctgg tggcgtggcg ctcgagcgcg agcgcgagct cgtcgcgcgc
    21001 gatgccgcag ccgtcgtcgg cgacgcggat gcgccgcacg ccgccctgct cgagctgcac
    21061 ctcgatcgcg cgcgcgccag catcgaccgc gttctcgagt acttccttga gcaccgaggc
    21121 cgggcgctcg accacctcgc cggcggcgat ctggttgacc agcaggtcgg agaggcggtg
    21181 gatgtggggc atgggggggc ggcgacgcaa aagcgggatt atacgggggt gcgggggcgg
    21241 cacagccttc cgccgctcgt gcagaaaacc ggcgattttc cttgcccgtg ccgggagcgt
    21301 ggcgcgggtc gggtatcatc gcggccatgc tccggggcgg gcgcccgccg gtccgcgcgc
    21361 ccaagccctg tcctccgcca ggatcttttc atcgttccag catcccatga agtccatcgt
    21421 catcctcatt tccggccgcg gcagcaatat ggaagccatc gtgcgtgcgg gcatcccggg
    21481 cgcgcgtatc gccgcggtga tcagcaaccg gccgggcgcc ggcgggctcg agttcgcacg
    21541 cgcgcacggc atcgccaccg cggtcgtcga ccacaagtcc caccccgacc gcgccggctt
    21601 cgaccaggcg ctcgccgagt gcatcgacgc ccatgcgccc gacctggtcg tgctcgccgg
    21661 gttcatgcgc gtgctcggcg acggcttcgt gcgccgctac gaaggccgcc tgctcaacat
    21721 ccacccctcg ctgctgccgg ccttcccggg gctgcacacc caccgccgcg cgctcgagac
    21781 cggggtgaag gtgcatggcg cgagcgtgca tttcgtcacc gccgagctcg acgacgggcc
    21841 gatcgtgatc caggccgcgg tgccggtgct gacgggcgac gacgaggaca agctggccgc
    21901 gcgcgtgctg gcgcaggaac acctcatcta cccgcaggcg gtacgctggt tcgtcgagga
    21961 tcggctcgaa ctcgtcgccg gccgcgtgag cctgcgcgac gcgccggccc aggacggcga
    22021 ggcgctgctg gtgcctgcgc ccgagggcgg agggcgggcg tgatgcgcgg gctggtcgtg
    22081 cgcctcgcgc tcgccgcctg cgcgagcgcg gcggcgctgc ccgtcggcgc agcggccgag
    22141 ccgcccgcgc agggccacat cgtctatgac gtgcactatg gctcgtccgg cttcaaggtg
    22201 ggcgaggcgc gccacgagtg gaagctggac aaggggcgct acgcgctctc gctcgcgctt
    22261 caggccaagg ggctcgccgg cctgttcggc ctcgaatacg agcagcgcag cagcggtacg
    22321 gtggacgcaa agggcatgcg cccggaactg ttcgaggtcg accagcgcgg gcgcaagccc
    22381 gagcgcgccg agttcgactg gactgcgggc aaggtcagca tccgccgcgg cggcgaggag
    22441 cggcgcaacg gcgagatccg cgctggcgac caggatctgc tcagcctgtg gcaccaggcg
    22501 cgcaaggtgg tcgagtccgg caagccggcg cgcttcgccg tgatcaccaa caagagcatc
    22561 aagcaggcgg tggtcgagcc gcgcggcgag gagcgcctcg agctcgccgg cgagcagctc
    22621 gacaccgtgc gcctgcacgc ctttgccgag aaggacgacc tcgacatcga catctggctg
    22681 tccaggcgcc atagcctgct gccggtccgc atccgtatca ccgacgacaa gggcggcgtg
    22741 ctcgaccagc gcgctgcgcg catcgaggcg ggcgtgcccg ccgccaggaa cccgaactga
    22801 aatgaaaccc aagaccgaaa agccccggac ccgggcgatc aagcggcagg agccggccgc
    22861 ggccgagcgc ccggccgagg gcgcggtgtc gcgcacgctc ttcatccagg ccacgcaggc
    22921 gctcggcgcc gtgctcgcct tcgactaccc ggccgacgcc gtgctgtcgc gccattttcg
    22981 cgacaaccgc gagctcggcc accgcgaccg cggcttcatc gccgaggcgg tgtacggtgt
    23041 gctgcgccgg ctgcgctggc tgcgccgcat cgccggcgcg gaggccacgc cgcgcatgct
    23101 gctgctggcc tggttcgcgc gcggcgaggg ctggccgatg cgcaacttcg agggcctggc
    23161 gagcgccacg gagcgcgact ggatcacctc gatcaaggcc gccgaggcgg gcgaacccag
    23221 cctggccgag cgcgccgacc tgcccgactg gctggccgag cgcctgctcg cgcagatgga
    23281 cggggacgag ctgctcgcgc tcgcgcacgg gctcaaccgc ccggcgccgc tcgacctgcg
    23341 ggtgaacctg atcaagagcg accgcgacgc gctgctcgag cgcctgcagg ccgacggcgt
    23401 gcgtgccgag cccggccggc tgtcgccgca ggcgatccgc ctggcgggca agccggcgct
    23461 gcagaagcat ccactcttcc tcgatggcag cttcgaggtg caggacgagg gtagccagct
    23521 gctcggcctg ctcgtgcagc cgcgccgcgg cgaactggtg gtggacttct gcgccggcgc
    23581 cggcggcaag accctgcagc tgggcgcgat gatgcgctcg accgggcgcc tgtacgcctt
    23641 cgacgtttcc gagaagcggc tcgccaagct caagccgcgc atggcgcgtg ccggcctgtc
    23701 gaacgtgcat ccggtgctga tcgcccacga ggccgacgcc aaggtcaagc gcctggcggg
    23761 caaggccgac cgcgtgctgg tcgatgcgcc ctgcaccggg ctgggcacgc tgcgccgcaa
    23821 ccccgacctc aagtggcgcc agtcgccggc ggcggtcgag gagatggtcg ccaagcaggg
    23881 cgcgatcctc gccgcggcgg cgcgcgtggt gcgcccgggc ggccgcctgg tgtatgccac
    23941 ctgcagcctg ctcgccgagg agaacgacgg catcgtcgac gccttcctgg ccgcgcatcc
    24001 cgagttccgc gcgctgtcgg cgcaggaagt gctggcggcg cagggtgtgg cgctggagac
    24061 cggcgagcgc ctgcgcctgc tgccgcatgt gcacgacacc gacggcttct ttgccgccgt
    24121 catggagcgt gccaagggtg ggtgagcgcg gacatgagcg ggcgcatcgg cgggcaggca
    24181 agcgccacgc gccgatgctc gggtggatgg cggcgctcgc cctcctggcg cttcccggcg
    24241 tacacgcgag cgagcgtttc gcgggcaagg gcgagatcga acttgccttc gctccgagcg
    24301 acgacaccga gaaggtggtg atcaacctga tccgcggcgc gcgcaagagc ctggaggtgc
    24361 acgcctacgc cttcaccagc cgcaacatcg ccgacgcgat ggtggcggcg cagcggcgcg
    24421 gggtgcgcgt cgaggtgctc gccgacgcgc agatgaatcg ccgcgagaag ggcaatgcga
    24481 tcccgcgcct gctcgccgcc gacgtgccgg tggccttcga gacgcgctac aacgccgcgc
    24541 acaacaaggt gctgatcgtg gacgccgaag gcccgcgctg cgcggtgctg accggctcct
    24601 acaacttcac ctggtcggcc gacaaccgca acgccgagaa catcctcatc gtgcgcgacc
    24661 actgcgagct tgcccgcgcc taccgcgcca actggctgcg ccaccgcaag gacgcgaccc
    24721 gcgtcacctc cctgccgtgg cgggagtaag gttctcgtag cagcgacgca ggtcgcgatg
    24781 cgggcaccgg aagacccctc ccgcggtatg gcggggtaac aaaggctgga ctgcatccaa
    24841 tccggaacgg ccttgcgtag aatcgtcctg ctgttttctc ttgtggacgt ttcctcgcat
    24901 gtacaacggt ctcttcgatc tcccctggtg gggctacgtg ctggtggcgc tcgcgctcac
    24961 ccacgtcacc atcgtctccg tcaccgtctt cctgcatcgc catcaggcgc accgcgcgct
    25021 cgagctgcat gcgctgccca gccatttctt ccgcttctgg ctatggctga ccaccggcat
    25081 ggtcaccaag gagtgggcgg cgatccaccg caagcaccac gcgaagtgcg agactgcgga
    25141 ggacccgcac agcccgcaga cgcgcggcct gcacaaggtg ctgtgggagg gcgcggagct
    25201 ctaccgcgcc gaggcgaaga atgtcgagac gctgcagcgc tacggccacg gcactcccga
    25261 cgactggctc gaacgcaagg tctaccgcca ctccatcctc ggcgtggcca tcatgctggt
    25321 catcgacctc gtcgccttcg gcccgctcgg gctgacgatc tgggcggtac agatggcctg
    25381 gatcccgttc tgggcggcgg gggtggtcaa tggcgtgggg cacttctggg gctaccgcaa
    25441 ctacgactgc gacgacgcct cgcgcaacct cgtgccctgg ggcatcctga tcggcggcga
    25501 ggagctgcac aacaaccacc acagcttcgc gacctccgcc aagctgtcgg cgaagtggta
    25561 cgagttcgac atcggctgga tgtacatccg cctgctcgag ctgctcggcc tcgccaaggt
    25621 caagaagacg atcccccggc ccaggttcgg cgaggtcaag ccggtgcccg acctcgacac
    25681 cctgcaggcg atcgtgacgc accgctacga cgtgatgacg cgctacgtgc gttcgctgcg
    25741 cgaggtcttc gtcgacgagg cgcgccggct gggcgaggtg cacgggctgg agctgagcgc
    25801 gcgtcggctg cggcgctggg tgctgggtgg cgagctgcgc ggactggacg acacccagcg
    25861 caggcggctg gagcaggccg tcgagggcag cccggcgctg cgcacggtgg cggcgatgcg
    25921 cgacgagctc gctgcgttgt ggtcgcgctc gaacgccagc cgcgagcagc tgctcgcgca
    25981 gctgcaggac tgggtggcgc gcgccgagcg cagcggcatc cgtccgctgc aggagttttc
    26041 gctgcggcta cgcagctatg cggcttgagc gaggggtggg gcgtcaggcg cgactcagtc
    26101 gcgcagggcg tcggcccagc agttgggggt ctcgtacagg cgcacgcgct ccagcctgag
    26161 gtggttgccg taggtgtcgc gataggccgg gtcgaggatg cggaaggcct ctgcggcgag
    26221 gttctcggcg gtgggcacga cgtcgagcac gacggtcttg tggccgggga tcgcggccag
    26281 caggtcgagc accaggtggt cgccccggta ggccaggaag gcgtggtccc aggggccgac
    26341 gacgtattcg ttggcgatgc gcttgacgtc ggcgaagtcc atcaccatgc cgttgaccgg
    26401 tacgccgtcg gccttgatga tgtcgcccga cagcgtcacc tccagcgcgt agcggtggcc
    26461 gtgcagatgc cggcactggc tggcgtggtc cggaatccgg tgtccggcgt cgaattcgag
    26521 gcggcgggtg atgcgcatgg cgggtctctc tggaaaggct ccggagtata gcaccggcgc
    26581 gcccgcgccg actccgcgta ttttccgcaa gtggttgaac aaacgatgaa ttcttcccaa
    26641 ggacagcccc ccggcacgct cgcgaccctg acggtgggca agcacgatcg cgacctcgcc
    26701 gggctgacct acgtctatcc ggtgctgtcg cgccgcgccg gcggggtgtc ggtcggcatc
    26761 aacctgaacc cgaacaacgc ctgcaactgg cattgcgcct actgtcaggt gcccgacctg
    26821 gtgcgcggca aggcgccgga gatcgacctc gcccggctcg agagcgagct gcgcggcttc
    26881 ctcgccgacc tcatccacgg cagctggatg gaacgccacg tgcccgaggg cgcgcgggtg
    26941 atccgcgaca tcgccttttc cggcaacggc gaggcgacca gcgccgaggt cttccccgag
    27001 gcggtcgcgc tggtgctgcg gctgcgcgac gagttcgggc tgtcaccgca ggcggtgccg
    27061 gtgcgcctga tcaccaacgg cagcctgctc gcgcaggcgc gcgtgcagga gggcgtgcgc
    27121 cggctgggcg aggcgggcgg cgaagtgtgg ttcaaggtgg atggcggcag cgtcgaggcg
    27181 atcgagcgca tcaacggggt gcggctcgag cccgcggcgg tggcgcgcaa cctgacgcgc
    27241 tgcgccgagc tgtgcccgac ctgggtgcag acctgcatgt tgcgctggga cggcgcgctg
    27301 ccgtcggacg cggacatcga cgcctggctg gcggtgctgg aagccgccgg gacggagcgc
    27361 ctgcgcggcg tgctgctcta cggtgtggcg cggccgtcga tgcagccgga ggcgccgcgg
    27421 atcgcgacgc tggcggcgga agaactcgag gcgatcgccg agcgcgcccg aaaaaaaggg
    27481 ctgacggtgc gcgtcagccc ttgatcgcgc gcggcatgcg ctcagcgctc cttgcgcttg
    27541 gcgcgtgctt ccttcttgag ggtcttgtag agctcggggt tggtcgcctt gaggtactcg
    27601 gcaacctcga cgtcgtcgcg cttgaccttg ttgatgtcga tctgctcgac atggaccagg
    27661 cgcagcaagg cctgcaccgg gcggggaatg ttgcggccgc tctcgtagcg cgagccaccg
    27721 ctctgggtca cgccgatctt ggaccagaac tgcgactggt tcaggccgag cttgcggcga
    27781 atttcgcgta catccagctt ttcatttgct ttcatggaga tttcctcgag tttgtatccg
    27841 ttcgggcctt cccggactcg atgcggacag gattctgcct atgactaagg tcatagaacg
    27901 catgcagtat aggttagtat tctctctaca tacaaaatag attttagcaa attcagcaaa
    27961 taagtaataa aaaacacgtc ctggagattt tgtttcgatc gggcgagcaa aacttcacgc
    28021 cgccggatag catcgcggac cttattcacc tgtagttttg gatacgacga tggcgctgat
    28081 agttcagaag tacggtggaa cctcggtggg gacccccgaa cgcatgcgga gcgtcgcccg
    28141 cagggtggcg aacttccggg cgcagggcca tcgcgtggtc gtcgtcgtgt cggcgatgag
    28201 cggcgagacc aaccgcctga tcgcgctcgc ccgcgcagtc gcgccaagtc cccgcacgcg
    28261 cgagctcgac gtcgtcgtat ccaccgggga gcaggtcagc atcggcttgc tgtgcatggc
    28321 gctcgacgac ctcggcgtcc cggcacgcag ctacactggc gcgcaggtaa ggattctcac
    28381 cgactcgacg cacacccggg cgcgcatcct cgacatcgac gcggcgccga tccgcaagga
    28441 cctcgacgcg ggcggggtgg tggtggtcgc cggcttccag ggcgtggacg cacacggcaa
    28501 catcacaacc ctcggccgtg gcggctcgga taccactgcc gtggcgttgg ccgccgcgct
    28561 cgaggcggac gaatgccaga tctacaccga cgtggacggc gtttacaccg ccgatccgcg
    28621 catcgtcccc gaggcgcgca agctcgatgc cgtccgcttc gaggagatgc tggagctcgc
    28681 cagcctgggc tcgaaggtgc tgcagatccg cgcggtcgag ttcgccgcca agtacaaggt
    28741 caagctgcgg gttctctcca gtttcgagga agacccggga gagggcacgt tcatcaccgt
    28801 cgaggaaagc agcaacatgg aaagaccggt catctccggc atcgccttcg tgcgcgacga
    28861 ggccaagctc accctcaccg gcgtgcccga ccggccgggt atcgcctacc agatcctcgg
    28921 tccgatcgcc gatgccgaca tcgacgtgga catgatcatc cagaacgccc cccgcgacgg
    28981 ctcgaccgac ctctccttca ccgtcgcgcg cgccgatctt gcgcgtgccg tgaaggtgct
    29041 cgaaggcgtg caggcccacg tcggcgcgcg ctcgatcgag agcgacgaca ccatgtgcaa
    29101 ggtctcggtg gtgggggtcg gcatgcgctc gcaccccggc gtcgccgcga agatgttccg
    29161 caccctggcc gaggagggca tcaacatcca gatgatcacg agctccgaga tccgcatctc
    29221 ggtggcgatc gaggagaagc acctcgagcg cgcggtgcgc gccctgcaca aggcctatgg
    29281 cctggatgtg gccgctgaat agatgcgttc gatcaacgtt tgaccggcag gcgcgaactc
    29341 gctatgatcg cgccttcctg atggagacgt ggccgagagg tcgaaggcac tcccctgcta
    29401 agggagcatg cgggctaaaa ctcgcatcga gggttcgaat ccctccgtct ccgccagagc
    29461 actcaagccc ctgatccata aaggggcttt tttgcgtcca cagtgcgcag agcggatgcg
    29521 cccgaagcat cccgcggcgg cctgtgccgg acttccaact tcccgcccgg tgacccccat
    29581 gcgacacgac ctgaaggtgc ccttcgccga aaaggaccag gcgaaaaagc tcggtgcgcg
    29641 ctgggacgct gcgcgcaagc gctggtacat cgacggcggc ctcgatccgg ccgtgttcgc
    29701 gaggtggcag ccgatgccgc acgacacggc tgcggcggcg tcgaggcgag gcgcgcctgc
    29761 cggtgtcgag ggcagggtgc aggtcggcag ccgcttcgtc gcgttgccgc gggtgtgcga
    29821 ctgcctgccc tgggatgatt gcgacgtctg ccgcctgcag gcgtggccgc aatagacggg
    29881 gggtgcaggc atggaagcgg tggcggtgat cgacttcgag accacggggc tgtcgcctgc
    29941 gcagggcgac cgcgcgaccg agatcgccgc ggtgatcgtg cgggacggcc gggtggtgga
    30001 tcgctaccag agcctgatga acgcgggcgt gcgcatcccg gcccatatcg aatccctgac
    30061 cgggatctcc aacgcgatga tccgcagcgc cccgactgcg gcgcaggtga tgcgcgaggt
    30121 cgcggacttc atcggcggca tccccatggt cgcccataac gccgcattcg atgcgaagtt
    30181 ctgggatgcc gagctcgctc gcatcgagcg cgcgcgcaag caggacttcg tgtgttcgct
    30241 gctgctgtcg cgccgcctgt ttccgcttgc ccccagccac aggctcggcg cactggtgga
    30301 gtacgccagg ctgccggtcg ccggccgctt ccaccgtgcg ctcgccgacg ccgagatggc
    30361 ggccagcctg ctgctgcgcc tggaagagga gctgcgccag cgccaccggg tcgcgcaggt
    30421 gtcggtggag ctgctgtgtc gcatccagcg cgcctcgaag gcgcaactgc ccaagtgcct
    30481 ggcggctgca cgtgaagggt gagcgaacag gcacgggtga gcggccgggc gcggggctcg
    30541 cccgctccca tcggtgtccg catccgccgc gctgtgtccg cgcagctcag gccgcggcgg
    30601 gggtgcggca gccgatcaga ttcatcgcga gcatgctcag gaccaccctg tcgtgctggt
    30661 tgagcacctc gatcttcgag cgcaccaggc cacggtcggg cttggaggcc gaacgccgcg
    30721 cctccacgat ggtgacccgc gtgctcagca cgtcgtccgg acgcaccggc tcgagccagc
    30781 gcagctcgtc gatgccgggc gaggccaggc tggcgttgtg ggtgaggtaa tgctccgagt
    30841 acatccgcat catcatcccg caggtcatcc agccgctggc gatcagaccg ccgaactgcg
    30901 tatccgcggc cttcgcgggg tcggtgtgga aatcctgcgg gtcgtagcgg tgcgcgaact
    30961 cgatgatctc ctgcgcggtg acgcgcatct cgccgaagcg atgcaccgcg ccggggacgt
    31021 agtcctcgaa atagcggtcg tcgatcgggg tggagaaggt gctggcgggg ggcgtggtgt
    31081 ccttggagtt catggtgccg tgctcgtgtg atggatcggg gcgatgttag cccgaagcac
    31141 ggcgagggcg ggcgatcgtc gtggccggtg cctgcgagat cgtgccgcgc gagcgctcgc
    31201 agtcgggccg cgcgggacgc ataatcgcga cctttccgtt tgtgcgcgcc gagtgcatcc
    31261 cgttccgcga gcgaccccat gacccagcct ccccgcgcct gcggcatcga cttcggcact
    31321 tccaactcca ccgtcggctg gctgcgcccc gacgcgccca tcctcgtccc gctcgaggac
    31381 ggccgcgcga cgctgccgtc ggcggtgttc ttcaacgccg acgaggattc gatccagttc
    31441 ggccgtgcgg cgctcgccga atacctcgag ggctacgaag ggcggctgat gcgcgcgctg
    31501 aagagcctgc tcggcaccag cctgatcgac gggcagaccg aggtgaatgg ccgcgcgctc
    31561 ggcttccgcg agctgctcgc gagcttcatc ggcgaactca aggcacgcgc cgagcgtgcg
    31621 acggggcgca ccttcgacca tgcggtgttc ggccgcccgg tgcacttcgt cgatgaggac
    31681 gagaccgccg accgcaaggc ccaggacacg ctcgaggcca tcgcgcgcca ggtgggcttt
    31741 cgcgagatcg gcttccagtt cgagccgatc ggcgccgcgc tgcactacga gcgcaccctg
    31801 agccgcgagg agctggtgct gatcgccgac atcggcggtg gcacggccga cttctccatc
    31861 gtccgcctgt cgccgcagcg cgcccgcgcc gtcgagcgcc gcgaggatct gctcggcaac
    31921 gcgggcatcc acatcggcgg caccgatttc gaccgcgtcc tgagcctgga ctgcgtgatg
    31981 ccggtgctcg gctaccgcag ccgcctgcgc aacggggcca cgatgccggc gagcgtgtat
    32041 ttccagctcg ccgcctggca cacgatcaat ttcgcctaca cccgtcaggt ctgggccgac
    32101 ctgcagcagg tctaccgcga tgcgctcgcg cgtaccgagc tcgatcgcct gtgccggctg
    32161 atcaacctgc gcgaggggca ttggctcgcg atgcaggtcg aggaggccaa gatcgcgctg
    32221 tccgcggcgg agcgcacgca ggtggtgctc gaccgcatcg agcccgggct ggtgctcgag
    32281 ctttcgcgcg cggcctttac cgcggcgacg caggggctgg tcgagcgcgt cggcggctgc
    32341 gtggccggcc tgctcgcggc ctccggtgtc gaccgggagc gcatcgacac gctctatttc
    32401 accggcggtg cgagcggcgt gccgcagctg cgcgcgcgca tcgcggccga gctgccgcgg
    32461 gcgcgatgcg tcgagggcga cctgtacggc agcatcggcg ccggcctcgc ggtcgaggcg
    32521 gcgcggcgct atggctgaag cggcccctcg ccacgccggc tggcgaggat cagcaggttg
    32581 cgcggcgtca gcgcgcgctc gcagaaggtg ccgatcctcg ccgtgaagcc gcgcgcctcg
    32641 aagccgagca cgaggtcgag cgcgagccac acctcgagcg cgcgccggaa ggcatgacgc
    32701 accagctcca ggcggcgcac ctcgtcgcgc cggcgctcgc cgacggcctc ccattccgcc
    32761 cagtccagcg caggcggcag gcgcaggccc tcgcgctcgg cgaggcggcg gcagaagtcc
    32821 tcgaagctgc cgttcagcca ggctggcggc accgggcgga agtggggcac ggggccgccc
    32881 tcggcggtct ggcgcagcgc gatgaagccg agcttccagg cctggtcgcg ggcgaggcgg
    32941 cggcggtcgt ggcgcggcgc ggtcacggtc tcggtcaccg ccaggcgcag ggttgcggca
    33001 tcgagctcga ggctggcgtc gcgtccgagt gcccgatagc cgccgttggc gccgcggtgg
    33061 tagcagcacg gggcgatggc gtaggcggcc gcgtcgtcgc ggtcggcgct gcgtaccagg
    33121 ctgcgatgca gctccccgca ggcgtgcagc gcgagcaccg catggccgtg caggtgaggg
    33181 cgggaggcgg gatcgagcgc gtccgcgcac accacgcgct gcggcaagcg gctgcgctcg
    33241 gcgagccgct ccgcatccgc gcacagggcc gggtcgatct ccagcgaacg caccggcacg
    33301 ccatcggcga gcgccaggcg gcggccgagg tggcccttgc ctgcacacca ctcgagcagt
    33361 ggcaggccgg tggcgggcag acgcgcggca aaagcttcga tctgcgctcg cttgcgtccg
    33421 ggcacgccgg catcgaagcg gcggtcgggc tcgggcagcg cgcgcgccgg cagggccggc
    33481 agcgcgcaca attcgacgct gcgcaggagc gcgggcaggt ggggggcgag ccagcgcagg
    33541 gcctcgtccg gctcctgcac cagctgctcg agcgtggcct cgtcgagtgc acgcagctcg
    33601 gcggcgagtg ccggccgctc gaagcaccag ggcgggaggc cggcctggaa ggcgggcgga
    33661 cgccacagcg cgtcgtggct ggcgagcagg gcctgcagtt cgaggaagcg ttcgcggtgg
    33721 ttcatcgtgg gaggaaaggc gcgcccgata cgcgcgccgg tgggcggaag gcggtggcgg
    33781 cggcaggcat ccgacccacg ggggcgcggc gagggcgggg gccgcgaacc ttcgatccac
    33841 cgcccagccc accgccttcc ggaccacgct agaacggcat taccagcttg agcaccgcct
    33901 tgtcgccgcc gaagcggttc tcgcgctcga actcgtgctg ccattgcgcg atcagcatcg
    33961 taccgccttt ggtcgagtag gtcaggctgg gaccggccgc gagcacctcg ccccggcgtc
    34021 cgcgcgacca cacccccggg atggcgggga tgaccgtgcc gtcgaccttg tcgtccgtgg
    34081 tctgcttgag gtagtagccc gacaggccca cgccccaggg gccgaagcgg cgcccgacga
    34141 ggtagtccat gtggaagtcg tcgccggagt cgtatttctg cttcgccgcg ccggggcttg
    34201 cgcggaagtc gtcgttgccg cccttgatgt tgtacatcag cttggccgag acctcccagc
    34261 cgctctgcga cagccaggtg aaggcgaaga tcggttcgag gctccagtag ttggccccga
    34321 tgctcgtacg cggatcgccc gacttgtagc gcccgatcgg caggttgatg tccagcccca
    34381 ccgtccagtg ccagtcgccc tggtgccagg cgacgatgaa ggggtcgatg gtgaggtcgc
    34441 ccagcccgct cgccgaggcg ctgccgccgc cgaggttcag gcgctggtgc accacgggca
    34501 ggatcacgtg gcccgcccag tccccgccca gcaccttgat gccgctcacc ttgacgaagc
    34561 gaaaggcgtt gaaccaggcg ctcgcggagg tgccggcgac gcgcttgccc tcgccgttgt
    34621 agaggtcgcc gccgtaatgg ccgaagtagt tgatgaagta gttgcccggc ggcggcaccg
    34681 cgccggcgaa ccaggtctcg attccgttcg gatactggtc gccgccttcc ttggcgccgg
    34741 ccgtgcccgc ggcgagcgcg ccgagcgcga cgaggaccgc gttgcgaagc tgcttgttca
    34801 tgaagtctcc tccctgtgtt tttctgtcaa tgcgatgcga tgcgatgcag gccgcggcct
    34861 cgggttgcgg ccccggggcc gcgggcgcct cagaacgggt agtggcgcgg gctcgtctgc
    34921 agggtgatcc agcgcagctc ggtgaaggct tcgatcccgg cctggccgcc gaagcgtccc
    34981 cagcccgaat ccttcacgcc gccgaagggc atctgcgctt cgtcgtgcac cgtgggcccg
    35041 ttgatgtggc agatgcccga ctggatgcgc ccggccacct gccaggcgcg cgcgaggtcg
    35101 cgcccgaaca ctgcggcggc gaggccgaag gggttgtcgt tggcgcaggc gatggcggcc
    35161 tcggtgccct ggacgcgcac gatcgccttc accggggcga aggtctcctc gtggtagatg
//
LOCUS       DAOQBS010000017        35039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_654_length_35039_cov_17.115939, whole genome shotgun sequence.
ACCESSION   DAOQBS010000017 DAOQBS010000000
VERSION     DAOQBS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 35039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35039
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_654_length_35039_cov_17.115939"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PKN25_03675"
     CDS             complement(<1..673)
                     /locus_tag="PKN25_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV90026.1"
                     /translation="MPAFILRRLLQSVLVMLTVALVAFGLFRFIGDPVNNMVGQEATM
                     AERAALRESLGLDDPVPVQFLRFVVNAAQGEFGVSYRMARPVADVIAERLPATLELAL
                     ISGFGALALGIALGVYTALRRDGWLSHAIMSLSLVGVSLPTFLIGILLIYVFAVELKL
                     LPAFGRGEVVDLGWWTTGLLTTSGLKSLVMPAITLGLYQLTLIMRLVRAEMLEVLRTD
                     YIKFAR"
     gene            complement(841..2436)
                     /locus_tag="PKN25_03680"
     CDS             complement(841..2436)
                     /locus_tag="PKN25_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV90027.1"
                     /translation="MNKTRLALALPFALATLASVAAAPAAAQTVRWAAAGDALTMDPH
                     SQNEGPTHVMNHQVYDSLVFRDQAMKLAPRLATAWKITEDPNVWEFKLREGVKYHNGN
                     PFTADDVVFSIQRAKHENSDMKGLLTSVVEVVKVDEHTVRMRTDGPNPLLPNNLTNLF
                     IMDREWSEANKVMLPQNYKAGDETFAVRNANGTGPFRLVKREPDVRTELERNEDYWGK
                     GTYPMEVAKVVFTPVRSAATRVAALLSGEVDFLLDPPVQDLERLSAAKGIVVRSGPEN
                     RTIFLGMNQGAAELRSADVKGRNPFADKRVRAAMNIAINRDAVKRVVMRGQSVPAGIV
                     APPFIDGYDKAMDVVPAPDVARAKALLAEAGYPNGFAVTLSCPNDRYVNDEAICQAVT
                     GMFGQIGVKARLDARPKSIHFAELPKGELDLYMLGWGVPTMDSHYVFHYLYETRTDKG
                     GSWNVTGYSSAKVDELTKAMNREIDLGKRAGMVAEVWKTVQDDVVYLPIHHQMLNWAM
                     KDDIDFPVQSENYPYFKLLKYRK"
     gene            complement(2652..3113)
                     /locus_tag="PKN25_03685"
     CDS             complement(2652..3113)
                     /locus_tag="PKN25_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNV90028.1"
                     /translation="MDFHPLLLFVHVAGVVVWVGGMFFAYVCLRPEAASQLEPPPRLR
                     LWRGVLARFFTWVWVAVAAVLASGLALLLRIGFAAAPPHQHLMLLMGLVMIAIYVFVY
                     SVPYAALSKAVAAEEWKAGGAALGRIRQLVGTNLTLGFLTIAMGTIGRWLG"
     gene            complement(3196..3828)
                     /locus_tag="PKN25_03690"
     CDS             complement(3196..3828)
                     /locus_tag="PKN25_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNV90029.1"
                     /translation="MSTTRIVVIAKAPQPGRVKTRLIPALGAEGAAALAARMLACTLV
                     AAFEAAGAADTADPALGVVELCASPAPGDPAWQGVVVPAGLRWSAQGEGDLGARMAGA
                     ARRVLAGGERVLLIGTDCPALDAACLRTAAEALDEVEAVIVPSFDGGYVLLGLRRFDP
                     SLFEGIAWSTSTVAAATRVRLDALAWSYQVLPALQDIDVPADLVHLPSAG"
     gene            complement(3825..4508)
                     /locus_tag="PKN25_03695"
     CDS             complement(3825..4508)
                     /locus_tag="PKN25_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNV90030.1"
                     /translation="MEGRPVSLSIVVPMVDEAATLPELLTHLAAWRDRGCEIVLVDGG
                     SRDDSVAIARAAGFRVMVAERGRARQMNAGAAACGGDILLFLHADTRLPEDADAKLRA
                     VQAGHAWGRFDVRIDGRPRMLRVVAALMNLRSRSSGIATGDQAIFVRREVFEAVGGFP
                     DQPLMEDIELSRRLLRVSRPACLRARVRTSGRRWEQRGVWRTIVLMWRLRWAYWRGVP
                     AERLAEAYR"
     gene            complement(4526..5539)
                     /gene="arsS"
                     /locus_tag="PKN25_03700"
     CDS             complement(4526..5539)
                     /gene="arsS"
                     /locus_tag="PKN25_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327695.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNV90031.1"
                     /translation="MHATLPLLESHRFPPLRREAITTLQVNLGYRCNQSCVHCHVNAG
                     PGRTEMMDADTLALIPRVLAARAIGTLDLTGGAPELHPGFRALVREARALGVRVIDRC
                     NLTILSEPGQEDLAEFLAEHGVEVVASLPCYAADNVDRQRGKGVFERSIAGLLRLNAL
                     GYGQAGSGRVLNLVYNPQGPVLPPDQARLEADYRRELAAQFGIVFNQLFALANMPIQR
                     FGSMLISKGQFNAYMALLRASHRDENLGGVMCRTLVSVDWQGRLYDCDFNQQLGLPLG
                     AEGALTSAQGHDDGDAAARPGRLHLRDLLAGDLAGAPIRVADHCFGCTAGSGSSCGGA
                     LKA"
     gene            5716..6864
                     /locus_tag="PKN25_03705"
     CDS             5716..6864
                     /locus_tag="PKN25_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV90032.1"
                     /translation="MKRLLLVGGGHAQLAVLQALARRRPHGVEAVLLTPSPWQWYSGM
                     LPGWMAGHYRAEDCRIDLRALADAAGVRLCLASAIALDAERRMLLDDTGTTHDFDLLA
                     LDVGSLVDTRALDALPTDALLAVKPLEAFIAAWPQRLAMARAGQLRRLAVVGGGAAGV
                     ELVLAAAEAFRRADCAVALALVAPPGGLLAGHAEGARWRVLRRLAAAGIALHPARARG
                     TPAGLELVDAGRDAPTTLPADCVIAATGAHAPPWTQSSGLACDAAGWVMVDAGHRSVS
                     HPFIHAAGDVCTRADPGFARSGVHAVHAGPVLAANLLAALAGGPASAPVRSYRPRPRS
                     LYLIASGPRHAIASWGRWSAEGRWVWRWKDRIDRGFIARHRRAAIVPV"
     gene            6934..8043
                     /locus_tag="PKN25_03710"
     CDS             6934..8043
                     /locus_tag="PKN25_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV90033.1"
                     /translation="MHELVQDYYGRTLQSSADLRTSACCDASAPPPALRPVLGRIHPE
                     VLARYYGCGLVSPPLLEGCRVLDLGCGSGRDVYALAQLVGERGEVVGVDMTAEQLAVA
                     RRHEDYHREAFGFARSNVRFLEGYIERLGELGLEDASFDVIVSNCVINLCPDKAAVLR
                     EVFRLLRRGGEFYFSDVYADRRVPAAVRDDPVLYGECLGGALYWNDFLALARAAGFAD
                     PRLVEDRPLAATDPALAAKLGDVRFFSATWRLFRIDGLESACEDFGQAVVYRGTLPEQ
                     ATRFVLDKRHGFDAGRVVPVSGNTWRMLHDSRLRPHFDFIGSFERHYGRFTAEGPAMP
                     FDTAARGTVTDNPAPTTMPAPVPATLGSAARKSCC"
     gene            8090..10258
                     /locus_tag="PKN25_03715"
     CDS             8090..10258
                     /locus_tag="PKN25_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV90034.1"
                     /translation="MKKPLLLLLVLALVAGFFASGLHRQLDLDTLKAGMAGFAAWREA
                     SPVLVAALYFAAYVAVTALSLPGAAVMTLAGGALFGLGWGLLIVSFASTIGATLAFLV
                     SRHLLRDSVHARFGARLRAIDEGIARDGAFYLFSLRLVPAFPFFLINLLMGLTPIRTR
                     TFYWVSQLGMLPGTLVYVNAGTELGAVDSLAGVLSPGLVASFVLLGLFPLLARWMVER
                     VQARRVYAGWQRPARFERNLVVIGAGAAGLVTSYIAAAVKAKVTLVEAGRMGGDCLNT
                     GCVPSKALIRSARLAHQIRHADRYGLEPGEPEIDFRRLMARVRAVIRKIEPHDSVERY
                     SALGVEVLQGRARIVDPWTVEVALNEGGTRRLSTRSIVIATGARPTVPDLPGLEAVGY
                     LTSDTVWDAFAALDAPPRRLLVLGGGPIGCELAQACARLGSQVVLVGRAPQLLPREDA
                     DVGAFARAALEADGVEVLTGVAALRCEQETAAGGTDKFLVVADPASGGERRIAFDTLL
                     CAVGRSARLEGFGLEALGIPTGATVATDDFLQTRYPNILAAGDVAGPLQFTHVAAHQA
                     WHAAVNALFGHLRRFRVDYRCVPHTTFTDPEVARVGLNETEAHARGIAFEFTRYDLAE
                     LDRAITDGADRGFVKVLTVPGKDRILGATIVGEHAGELLAEFVLAMKHGLGLNKLLGT
                     IHAYPTFAEANKSVAGEWKRAHAPQRVLRWLERHHAWRRG"
     gene            complement(10272..11525)
                     /locus_tag="PKN25_03720"
     CDS             complement(10272..11525)
                     /locus_tag="PKN25_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV90035.1"
                     /translation="MSRTEASRNMGGHAPSAAAGVPPAFVVIIAGMVAALHIGKIPPA
                     IPVLRDALGVSLVEAGFLLSMVQMAGMLGGVFIGLAADGFGLRRSVLLGQGVLASASL
                     AGAWATQPGELLVLRAIEGLGFLLVVLPVPSLIRQLVPPSRLALHLGLWGTYMATGTS
                     LVLAGGPALMGVMGWQGLWGLLAMASAAVAAWLAVRVPSDHARRAAAGTPVGAAVAAR
                     ARWGARLRATLGSAGPWRVAITFSMYSSQWLAVIGFLPSIYAQAGLSGQVAGLLTAFA
                     CLANVTGNVAAGRLLHRGVCARHLLYAGFLAMAATTFVAFSPLAAELPVARYLAVVLF
                     SAIGGLIPATLFALAVHVAPDDDTVSTTVGWMQQCSSAGQFLGPPLVAWVAGAVGGWQ
                     WTWVITGLASLVGLVLSSRTRYQRA"
     gene            11682..12515
                     /locus_tag="PKN25_03725"
     CDS             11682..12515
                     /locus_tag="PKN25_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNV90036.1"
                     /translation="MTTPHPEIRPGQSVELLKQLHILTRDGKLNQDSRRKLKQVYHLY
                     QFIEPLLAEALAERPDIELVDHGAGKSYLGFILYDLFFKAHAPGGRIHGIETRDELVT
                     SSRRLADKLGFSSGMRFQNLSVADSITSDRLPGRIDLVTALHACNTATDDAIRFALKK
                     GARAIVLVPCCQAEVAAALRRNKQQALRGPIAELWRHPIHTREFGSHVTNVLRCLQLE
                     AHGYEVTVTELVGWEHSMKNELIIARRQPERPRRRAAERLAALLGMLGLEELSERFFT
                     S"
     gene            12673..13668
                     /locus_tag="PKN25_03730"
     CDS             12673..13668
                     /locus_tag="PKN25_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV90037.1"
                     /translation="MAEIESPLAFLLTTPYASRYPRKLVEGFPHVARRIAELWDDKDK
                     LGEYFTELMVSKRPNRRGFPPEVGAEIVYLSMAYDLHGPVRPTPQAAPEPTTAARDDA
                     WDYERAVAELERLDIPITMAQFVRALEAGDQHLCSLFLHAGFDIDGRDARQWTPLMIA
                     CFHGREALALELIRLGASVDATDADGYTPLHWASVNGYQKVGEVLVRRQAEVNATSNA
                     GITPLLQAAARGHLGVVRLLLDRKAKVNLVAADGSTALLKAVANGHWEIVNTLLDAGA
                     STQATMKNGTTLVDIAARSKDERIRERIAIAARMEARGDAPIQRDEPPPLTGTIY"
     gene            13942..15120
                     /locus_tag="PKN25_03735"
     CDS             13942..15120
                     /locus_tag="PKN25_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNV90038.1"
                     /translation="MAVMPSAALAVLVVALTSLGPLSTDFYLPALPAIARALHTDSAG
                     VQLTLSVYLLGFGAGQLLVGPLSDRCGRRPVMLWGMLVFLLSTLVCVFAESLAVLVGA
                     RLVQALGACAGPVLGRAVVRDLYGPAESARVLSHVSTATALAPLLAPLFGGWLTAAWG
                     WRATFVALAIYAMVLMLAVAALLKETNRHPDAHAMRPARMLANYRTLLADPAYRGALL
                     IGCGAFAALFAFISGSPFVFIEHFGMSPQQMGLAFGLNVTGFMIGSTLSARHSRRLGP
                     ARLIRYGVWIGAACGVLLAALAFASPQHPAAVMLPMWGVTAAIGLILPNATALGLAGY
                     PKMAGAAASLMGFVQMGLGAGAGMLVGHGVQHGTAALGLTVAAGMLFALAAWSRSPAR
                     "
     gene            complement(15134..18205)
                     /locus_tag="PKN25_03740"
     CDS             complement(15134..18205)
                     /locus_tag="PKN25_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV90039.1"
                     /translation="MNHAPAADAATRGRHAGLRLAFPLIALTVLVGGVGAFGYRSLSE
                     EIRRETQRTLAVIAEQKRQHIEGWLAEARVDVRMVFTGHSQLEALFEAWLDGGRRDDA
                     AFARMRALVEELARLRGWQGVALLDADGAPALAVGSPDLSAHAELIADVLRQPRVELV
                     DLHQDARGRTHYGVLAPVGAPSRGVAYLTWEAETALYPMVEAWPVPTRSAETYLVRRE
                     GEGVRFLTPLRHQADAALALERPLATPDLPAARAALGERGILSGGRDYRGVPVLAYAA
                     AVEGTPWLMLAEIDEREAYSGIRTLTWGMGVVMALGLMLVYSGGYLVWRRDREQRERA
                     TLQARQIAEARFRVIFEQAPLGVVLLDPRTRRITEANPRFAAIVGRSVGELVGVDPIA
                     LTHPDDVAESLRQLGRLDAGRIAGYRLNKRYLRPDGTPVWVSLAFAPVQVASEDAPRY
                     LGIVEDISARIEMEERLREASAAAAAANAAKSEFLAHMSHEIRTPMNAVLGLAQVLER
                     EPLAPAQRDMVGRIRGAGASLLAILDDVLDLSKIEAGQLRIEPRPFDLRALLANLDSL
                     MGQAARAKGLALRIEPPALPPGQLRGDGLRIEQILINLVSNAIKFTERGEVSLRVRAD
                     EVGEVRLRLRAEVRDTGIGIAPEAQARLFAPFTQADAGIARRFGGTGLGLSICKRLVE
                     LMGGAIGVHSQPGLGSTFWFELPLERVAGGEPASVGVVAAPEGDRAAGPRLAGMQVLV
                     VDDSAMNRDLVQRALALEGACATLAADGQQAIELLRGRPQAFDAVLMDVQMPVLDGLS
                     ATRRIRDELGLAALPVIAFTAGVGEDQQAAARAAGADDVLPKPMDLEQMTQLLMRWVM
                     PQPAAGLAEATPAAGAGDRPAHAVAAAPMPAAAPVPPPASPAQSAAAPPAAAGDDFPA
                     LPGIDRERAMQRLGKDRDMFIGLLGLFIEDNAGVVAATRADLARGERESAARRMHTLR
                     SNAGFICALAIMQAAAALEKAIAQDEPDVAARLDELAADIAGLVEAGRAFL"
     gene            complement(18307..19176)
                     /locus_tag="PKN25_03745"
     CDS             complement(18307..19176)
                     /locus_tag="PKN25_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNV90040.1"
                     /translation="MRSFPSCGWRIRLARVRLARVLAAAAACVALPVAALELPRERAV
                     AGGVALVELGPALVAPQASLDGVPVLVAGRPGGWTAVVGIALEAEPGSVDLEVRAEGE
                     PPRRVPVAVGPVRYAEQRLKVAPGKVDLSPADLARHERERLRSAEIVATWSTLDPGSL
                     RMLQPVPGRRSGSFGLRRVFNDQPRKPHGGMDIAAPTGTPVVAAAAGRVIDTGDYFFN
                     GNTVWLDHGRGLLTMYCHLSSIAVKAGDTVKAGARIGAVGATGRVTGPHLHWSVMLNR
                     SMVDPALFLPGGG"
     gene            19391..20836
                     /locus_tag="PKN25_03750"
     CDS             19391..20836
                     /locus_tag="PKN25_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HNV90041.1"
                     /translation="MEAFANQVVDLLNGLLWGKILIWLLVGTGIYFTLRLGFIQLRHF
                     GHTFTVLRGSRQSDASGISSFQALCTSLAARVGTGNIAGVAVAITLGGPGAIFWMWVI
                     ALVGMATGFAEATLAQLFKQRDDKGQFRGGPAYYMEKGLGQRWAGTMFSIFLIIAFGF
                     AFNSVQSNTIAGAMVGAFELDMGSFTLGGNEVAVAALVVGVAVTVLTAVIIFGGLRSI
                     ARFSELAVPFMAVGYLVVAVGILLVNLGEVPGVLAMIVKSAFGFHEAAAGGIGAAILN
                     GFKRGLFSNEAGMGSAPNAAAAATPYPPHPASQGYVQMAGVFIDTILICTASAAIILL
                     AGPVEGTGVGLVQNALTSEVGGWGKYFLAVVVLFFAFTSIVANYFYAENCLVFIEHNH
                     PAGLLIFRLIVLAMVMFGAIGSLPFVWNFADVAMGLMAITNLVAILLLSGLVVKLAKD
                     YNAQRAAGKLPTFDARAYPEIRSKLTPGIWD"
     gene            20920..21780
                     /locus_tag="PKN25_03755"
     CDS             20920..21780
                     /locus_tag="PKN25_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNV90042.1"
                     /translation="MHSLLEFPSPFPAEPGVVRLLEPPGSDAAALAARLVAGDYGKPF
                     VLENGGLRALHFSLGYVQSVMDIANPSALVLAYARAMMAFLLFNPRPRRIGMIGLGGG
                     SLANFCHRHLAAAELTVVEIDADVIALRRHFGVPADDARFRVLHADGVRWLGETQTRS
                     DVLLIDAFGAEGVAHTLLDTDFHANAFRHLGGHGVLAMNLAGARESFAPHVARLLEIF
                     DERVIAIPVREDGNHILFAFRDPAFTPRWNWMRSQAHALQARTGLDFPNLARALEAGE
                     RQGHAWRLAR"
     gene            complement(21811..22086)
                     /locus_tag="PKN25_03760"
     CDS             complement(21811..22086)
                     /locus_tag="PKN25_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90043.1"
                     /translation="MKSHLAENIQIAHPRYHLSSDDGLYRPIPFLFVSPRMRDDILDE
                     REMLLSAQPAALHERQQKLFASYDPAVSMEAFRQLLRLYGYPFNNRR"
     gene            complement(22250..22525)
                     /locus_tag="PKN25_03765"
     CDS             complement(22250..22525)
                     /locus_tag="PKN25_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90044.1"
                     /translation="MKSHLAEDIRIASPRFHLPTRDGLYAPIAFLFVTERMRDDILNE
                     RSLLVASLPPALRARQQKLFDRYDPVAGARSFTELLELYRYPFAGSH"
     gene            complement(22672..23220)
                     /locus_tag="PKN25_03770"
     CDS             complement(22672..23220)
                     /locus_tag="PKN25_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HNV90045.1"
                     /translation="MTPIRAAALALVLTCAVPAAHALDDLRPGLWEFRSTRLSLAGLP
                     DMAAQMGMLQQHIKGLPADTRRMLEQQMQQRGVSLGADGSVRSCITPAQARQDNVFAG
                     KVEGNCTLTRVERSGNTIRGSLRCTDPDAEGDFHARVEGPEHFTTRVKLASVRGDLDL
                     ETDARWLGAGCGAAAVAPKPAR"
     gene            complement(23437..24549)
                     /locus_tag="PKN25_03775"
     CDS             complement(23437..24549)
                     /locus_tag="PKN25_03775"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNV90046.1"
                     /translation="MSADPVSVAGIDGPAALRDRALGALLGAFIGDALALGPHWYYDL
                     DELRARYGPWITGYTAPMPDHYHAGMKAGESSQAGVLLRLTMESLAECGGYDEADFCR
                     RMDEDFFPRLDGTPMQGPGGYTSQSIREAWRKRIRQGLPWGRVAGNADTTEAAERTLA
                     IAVRYALDPAALADAVTRNTVLTQTDGTVVAMTVAFGAVLGMLVEGHKFDAAISAKLM
                     ARVKSGALPFHTVTAGNLQAPRPGAAEAPTAGRFPSPDALLTQSSIARAAHDPDVRIE
                     PAWKVALVYGMPCAVYHQFPAAYYLAARFADDFESAVLHALNGGGQNLARTMLTGALV
                     GAQVGLAGIPARFVKGLVDHAALLALAGRIGPGRVL"
     gene            complement(24549..25277)
                     /locus_tag="PKN25_03780"
     CDS             complement(24549..25277)
                     /locus_tag="PKN25_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV90047.1"
                     /translation="MGVVKGVICHHTAGPRSGNMPSLGVLKNGRADLAGPLAQLGLGR
                     DGTFYVIAAGRANHAGRGEWKGITDGNGSFIGIEAESTGIGDPTKPGFEPWPEVQLDA
                     YRRGVAAILAHLGQDAGMCCGHKEYALPKGRKSDPHTLDMERFRSEVAALMSGAAAPV
                     PSIPRKDAEDRPTIRRGATGNPIFLVKEVQRKLGFGAQQVDGAFGPLTEAAMRRFQRA
                     HGLVPDGIVGPRTWKALDTVAIGP"
     gene            complement(25375..25500)
                     /locus_tag="PKN25_03785"
     CDS             complement(25375..25500)
                     /locus_tag="PKN25_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90048.1"
                     /translation="MNGTTEKREPDEQEIDVCDRPVQETEATPDEDLPAAEGGVA"
     gene            complement(25612..26235)
                     /locus_tag="PKN25_03790"
     CDS             complement(25612..26235)
                     /locus_tag="PKN25_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90049.1"
                     /translation="MKRAKTLWMVALAVLLLVAGLYAGERRGVPAPAPAPARTAVEEI
                     VLVANEVVEAFVRRDGERLAGRAHPHKGVRFSPYAYVDVEEDRVFLPAQLRHFWDDAT
                     VHVWGHADGSGEPIALTAAQYVERFVLDRDYRGAGRIGVNVDQQRGNTADNAAQAYPQ
                     GTRVEYYVEPAPGGGAAAFDWSALRLVFEQVDGRWLLVGVIHDQWTT"
     gene            complement(26248..27390)
                     /locus_tag="PKN25_03795"
     CDS             complement(26248..27390)
                     /locus_tag="PKN25_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNV90050.1"
                     /translation="MERIIPCTDCAEKTLDVEQLGFRVTSCDPHPERPGFCVLRFEDR
                     SATPAAGASLAAPAAAARAGRGAVAGGVTATQAAVAKAIVNLFETGEVLGQYGKVTLI
                     PGDTGHLTFGRSQTTLGSGNLAKLLQQYCANPGARFAGRLASYLPRFLAIDESLDDDP
                     RLHNVLRATADDPVMRDTQDAFFDRTYWEPALRAAASLGVHTPLGVAVVYDSAVHGSW
                     LAMRDRTTRAVGEPAAVGEQAWIDAYVRTRRAWLEGHARADLRQTVYRMEAFGRLIDQ
                     GFWGLEMPLVVRGREISSVTLAALPPGCYDGPQPGSRPLTLATPLARGLDVRLLQLGL
                     SDRGVDILADGIFGRTSFNLLKAWQAQHGLAATGIADPALIGELTA"
     gene            complement(27415..28335)
                     /locus_tag="PKN25_03800"
     CDS             complement(27415..28335)
                     /locus_tag="PKN25_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90051.1"
                     /translation="MSTRRVSILLSLALMVAGCASRPDTVVFVTKSSLGVDVEQTPPS
                     ASIAYDRVEGYFGPRYESGAVPPVLAVFMTNGQLLDRQVKQLYATGDAARALLAPAAP
                     LPSDPEQGGDFKPMFFGSSTTVGLKIGYEAGGSTSTFTLGYKRKELSVIPVNAGRFPS
                     VLATLQTDVDARSATDSRFGAGQLFATGAAATALAARPEVRDVFLTKADAALNAYRSE
                     ERQQSRHALVTLGCLAALPDAKLPAVWRNAEALGVLDQEAGAGAFASLGPDAARERYT
                     RYLALLDADSADATTRLSLHRQYVCGLAGR"
     gene            complement(28332..28643)
                     /locus_tag="PKN25_03805"
     CDS             complement(28332..28643)
                     /locus_tag="PKN25_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90052.1"
                     /translation="MNPLVDSILRGGALAVLALAGGCAHVWVDAEGQRHLLGWMHLTL
                     PPAQGAVAGETLRVQTLGLGWTRAEAGSALVLGYADTTLGFLRDGACLAPAADRTEVV
                     R"
     gene            complement(28822..31005)
                     /gene="ovoA"
                     /locus_tag="PKN25_03810"
     CDS             complement(28822..31005)
                     /gene="ovoA"
                     /locus_tag="PKN25_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HNV90053.1"
                     /translation="MNPASQATPGDRPSPASSSAGSPAPLFPRTPLLSGTDVEAKRAE
                     LLHYFHATFDRYESLFEVLTCDEAYYRKPISLRHPLIFYFGHTATFFINKFMLAGLIE
                     QRIDPRLESMFAVGVDEMSWDDLDDARYDWPSVAEVAAYRGKVRAVVERVIRDMPFTL
                     PIGWKDPFWAVVMGIEHERIHLETSSVLMRQHALQYVKPHPAWQPCAEHGEPPANALV
                     DIPAGTVTLGRSFDEPIYGWDNEYGTHRAEVPAFQAARHLVTNREYLGFVEAGGYADD
                     SVWDEEGLGWRRFARAEHPTFWVPDAAGWKLRLMTEEVPMRWDWPVEVNCLEARAFCR
                     WKARETGQPVRLPTEDEWNRIHDHAGLADVPHDAPANANLHLDHWASSCPVTRFAHGE
                     LFDVVGNVWQWCETPTYPFDGFDVHPIYDDFTTPTFDDRHAIIKGGSWISAGNESRHA
                     SRYAFRRHFFQHAGLRYVVSEAPVINPASSYETDTLLSQYAEFHYGDEAFGVPNFPKA
                     LADIAIAAQRRFGKGRFGRALDLGCATGRASFELARAFERVVGIDFSARFIQAGVKLA
                     ETGVLRYTMPDEGELVTYHERRLDALGLAGTAGRVEFWQGDACNLKDVFTGFDLILAA
                     NLIDRLYSPRRFLADVPRRLKPGGLLLLASPYTWLEEHTKREEWIGGFKKDGESYTTL
                     DGLKDLLATDFELVQGPQAVPFVIRETRRKHQHTLSELTVWRKRS"
     gene            complement(31341..32639)
                     /locus_tag="PKN25_03815"
     CDS             complement(31341..32639)
                     /locus_tag="PKN25_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV90054.1"
                     /translation="MSGQFRLLRERRFLPFFLTQFLGAFNDNVYKNALVVLITFQAAR
                     LGADSPGVLVNLAAGVFILPFFLFSATAGQLADKYEKSRLIRATKLLEIAVMALAVVG
                     FALMSLPLLLVVLFLMGAQSALFGPVKYAIIPQQLADDELVGGNALVEAATFVAILAG
                     TIVGGLLVAGDAGPGRVAVAVLAIALLGWWSSRAIPPAAAADPGLRVNWNPVTQTREM
                     LRFMLEARAVFVAIVGISWFWFYGAVFLSQFPGFAADHLGGDERAVTLLLALFSVGIG
                     AGSLLCGRLSRGRVEPRMVLPGAIGLSLFALDLWWASPAAGAFPPGQGLDTLFARAEV
                     WRVVFDLVMIGVCGGFFIVPLYALVQQRSAPAHRARVIAGNNILNALFMVAAAAMGVG
                     LLAAGFAVPQLFLATALLNALVAALLFAREPAFRQRGGPP"
     gene            32926..33105
                     /locus_tag="PKN25_03820"
     CDS             32926..33105
                     /locus_tag="PKN25_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90055.1"
                     /translation="MTLPARLRSAHRRAALRAGVRVRARGIVSPLRDLATQLAFKVTG
                     SPAVLSQAVRGHQRG"
     gene            33336..34208
                     /locus_tag="PKN25_03825"
     CDS             33336..34208
                     /locus_tag="PKN25_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90056.1"
                     /translation="MVLTRSFVRAKRPCTDGFRWFVRQFGEGGDYQAMLDEMVAAGRV
                     SDACWLLEQFGPTDELRVVDAIEADAIVFAGTLEVRGGVDVDGVLRVGRSLQAGAGVR
                     AGGEVHIGADLRVEGSVRAEAGLQIGGDLRVGWGVDCAGELRCDGDLRTGWDLRCGGR
                     MRVAGNAYVGLDLQAEEGVRCAKGLQAGGDIQVENTLRAAQGIAAGGSIRSGMHLEAG
                     WGIKAGGVIAAAGAIRAGESLAAGEAIRAGTGYGVFAGLDVQMEAWEASARVSAPQRP
                     EGLMSGWWAGPSPC"
     gene            34223..35035
                     /locus_tag="PKN25_03830"
     CDS             34223..35035
                     /locus_tag="PKN25_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90057.1"
                     /translation="MNTLALHLRGLPARHTPLSHRASLSPRSVLAFPQPPPFVAGPAV
                     ALRTALGTALATVLRRMLRPPRMELRIDVSPPDTAIEAELDTLHGRLHTPGDALHACT
                     RLPTLLPDLVFHHREADGEHYVYVEDVARGCLAGYTVFNRLIEVDRRTDRHVRSPHSK
                     YAPAYQGRGIASAVYDWALGRGFCLVSGARQSAGAHALWHALARRHPLRWVALRTKRM
                     HDLGASVAPTRAAELDTRMILLGHGWSAARLRSLGLLHPAAEAANETMRRRA"
BASE COUNT         5038 a        12813 c        12269 g         4919 t
ORIGIN      
        1 cgcgggcgaa cttgatgtag tcggtgcgca gcacctcgag catctcggcg cgcacgaggc
       61 gcatgatcag cgtgagctgg tacaggccga gcgtgatcgc cggcatcacc agcgacttca
      121 gccccgaggt ggtcagcagc ccggttgtcc accatcccag gtccaccacc tcgccgcggc
      181 cgaaggcggg cagcagcttc aattcgaccg cgaacacgta gatcagcaaa atgccgatca
      241 ggaaggtggg cagcgacacc cccaccagcg acaggctcat gatcgcgtgg ctcagccagc
      301 cgtcgcggcg cagcgcggta tacacgccga gcgcgatccc cagcgcgagc gcgccgaagc
      361 ccgagatcag cgccagctcc agcgtcgccg gcaggcgctc ggcgatcacg tcggccaccg
      421 gccgcgccat gcggtaggac acgccgaact cgccctgcgc cgcattcacg acgaagcgca
      481 ggaactgcac cggcaccggg tcgtcgaggc cgagcgactc gcgcagcgcc gcgcgctcgg
      541 ccatggtcgc ctcctgaccg accatgttgt tcaccgggtc gccgatgaag cggaacagcc
      601 cgaacgcgac cagcgccacc gtcagcatga cgaggacgga ctgcagcagg cggcgcagga
      661 tgaaggcggg cattcggggg agcgggagtg aggaatcgga tgcggggtgc gagggagggg
      721 atgggagcgt gatcgcgggc gggggagcgg gggatcgcga ctggcgtcgc gcctacaggg
      781 cggtcgccgc gtccggtgag ctttctgtag gagcgacgca agtcgcgatc ccgcgggtca
      841 tcacttcctg tacttcaaca gcttgaagta gggatagttc tccgactgca ccgggaagtc
      901 gatgtcgtcc ttcatcgccc agttcagcat ctggtggtgg atcggcaggt agacgacgtc
      961 gtcctgcacc gtcttccaca cttcggcgac catgccggcg cgcttgccga ggtcgatctc
     1021 gcggttcatc gccttggtca gctcgtccac cttcgcgctg gaatagccgg tcacgttcca
     1081 cgagccgccc ttgtcggtcc tggtctcgta gaggtaatgg aagacgtagt gcgagtccat
     1141 ggtcggcaca ccccagccga gcatgtagag gtcgagctcg cccttgggca gctcggcgaa
     1201 gtggatgctc ttgggccgtg cgtccagccg cgccttgacg ccgatctggc cgaacatgcc
     1261 ggtcaccgcc tggcagatgg cctcgtcgtt cacatagcgg tcgttggggc aggacagcgt
     1321 caccgcgaag ccgttcgggt agccggcctc ggcgagcagc gccttggcgc gcgcgacgtc
     1381 gggcgccggc acgacgtcca tcgccttgtc gtagccgtcg atgaagggcg gcgcgacgat
     1441 gccggcgggc accgactggc cgcgcatcac cacgcgcttc accgcgtcgc ggttgatcgc
     1501 gatgttcatc gccgcgcgca cgcgcttgtc cgcgaagggg ttcctgcctt tcacgtccgc
     1561 gctgcgcagc tccgccgcgc cctggttcat gccgaggaag atcgtgcggt tctccggccc
     1621 ggagcgcacc acgatcccct tggccgcgga caggcgctcg aggtcctgca ccggcgggtc
     1681 gaggaggaaa tcgacctcgc ccgagagcag cgccgcgacc cgggtggcgg ccgagcgcac
     1741 cggggtgaac accaccttgg ccacctccat gggataggtc cccttgcccc agtagtcctc
     1801 gttgcgttcg agttcggtgc gcacgtcggg ctcgcgcttc accagccgga agggaccggt
     1861 gccgttggca ttgcgcacgg cgaaggtctc gtcgccggcc ttgtagttct gcggcagcat
     1921 gaccttgttc gcctcggacc actcgcggtc catgatgaag aggttggtca ggttgttggg
     1981 cagcagcggg ttggggccgt cggtgcgcat gcgcacggtg tgctcgtcga ccttgaccac
     2041 ctcgaccacg ctggtgagca ggcccttcat gtccgagttc tcgtgcttgg cacgctggat
     2101 ggagaacacc acgtcgtcgg ccgtgaaggg gttgccgttg tggtacttca cgccctcgcg
     2161 cagcttgaac tcccacacgt tggggtcctc ggtgatcttc cacgcggtgg ccagccgcgg
     2221 cgccagcttc atggcctggt cgcggaacac cagcgagtcg tagacctggt ggttcatgac
     2281 gtgggtcggg ccctcgttct gagaatgcgg gtccatggtc agcgcgtccc cggccgccgc
     2341 ccagcgcacg gtctgggcgg ccgcgggggc ggccgccacg ctggcgagcg tcgcgagggc
     2401 gaaaggcagg gcgagggcaa gtcgggtctt gttcattttt cggtctccgt cgggtgctgc
     2461 cgggattgag gaacgggtgg gccgggggac tgccggaccg gctcggcacc gagcatgccg
     2521 gtcgattgtt gcaccggccg ggccggccgg ccaagcacgc agcgtcagga tcagggttct
     2581 ccccgatccg gccgttgcga cgagcggtgt gcatgggtgg ccaccgcccc gccgcgaacg
     2641 ggcgctgcgg ctcagcccag ccagcgcccg atcgtaccca tggcgatggt gaggaagccg
     2701 agcgtcaggt tcgtccccac cagctgccgg atgcgcccga gcgccgcacc gcccgccttc
     2761 cactcctcgg ccgcgaccgc cttgctcagg gctgcgtagg gcacggaata gacgaacaca
     2821 tagatcgcga tcatcaccag gcccatcagc aacatgaggt gctggtgcgg cggcgccgcc
     2881 gcgaagccga tgcgcagcag caacgccaac ccgctcgcca gcaccgcagc caccgcaacc
     2941 cacacccagg tgaagaagcg cgccagcacc ccgcgccaca gccgcaagcg cggcggtggc
     3001 tcgagctggc tggcggcctc gggacgcagg cagacgtagg cgaagaacat cccgcccacc
     3061 cagacgacga cgccggcgac gtgcacgaaa agcagcagcg gatggaagtc catggggatt
     3121 ctccttcgtg ggatcgggaa cccgattgtg ccgcaaaagc cggcccgcac cggcggatcc
     3181 cggcttgccc cggcttcatc cggcggatgg aagatggacg aggtcggccg ggacgtcgat
     3241 gtcttgcagc gcgggcaaca cctgatacga ccacgccagc gcatcgaggc gcacccgggt
     3301 cgccgccgcg accgtcgatg tactccaggc gatgccctcg aacaggctcg ggtcgaagcg
     3361 gcgcaggccg agaagcacgt agccgccgtc gaagctcggc acgatcaccg cctcgacctc
     3421 gtcgagcgcc tcggccgcgg tgcgcaggca ggctgcgtcg agcgccgggc agtcggtgcc
     3481 gatcagcagc acgcgctcgc cgccggcaag cacccgccgc gccgcgcccg ccatgcgtgc
     3541 gccgagatcg ccctcaccct gcgcggacca gcgcaggcct gccgggacca cgacgccctg
     3601 ccaggccgga tcgcccgggg cggggctggc gcacagctcg accacgccga gcgctggatc
     3661 ggcggtatcg gcagccccgg cggcctcgaa agccgccacc agcgtgcacg ccagcatgcg
     3721 ggcagccagc gccgcggctc cctccgcacc gagcgccggg atcaggcgcg tcttcacccg
     3781 ccccggctgc ggcgccttgg cgatcaccac gatacgggtc gtgctcaccg gtaggcctcg
     3841 gcgaggcgct cggccggcac gccgcgccag taggcccagc gcaggcgcca catcagtacg
     3901 atggtgcgcc acaccccgcg ctgctcccag cgtcgccccg aggtccgcac ccgcgcccgc
     3961 aggcaggccg ggcgcgacac gcgcagcagc cggcgggaga gctcgatgtc ctccatcagc
     4021 ggctggtccg ggaaaccgcc caccgcctcg aacacctccc ggcgcacgaa gatggcctgg
     4081 tcgccggtgg cgatcccgct cgagcgcgag cgcaggttca tcaacgctgc caccacgcgc
     4141 agcatgcgcg ggcgcccgtc gatgcgcacg tcgaaacggc cccaggcatg gccggcctgc
     4201 acggcccgca gcttcgcgtc cgcatcttcc ggcaggcgcg tgtccgcatg caggaacaac
     4261 aggatgtctc cgccgcacgc cgccgcaccc gcgttcatct gcctggcgcg gccacgctcg
     4321 gcgaccatca cgcggaagcc ggctgcgcgc gcgatcgcca ccgagtcgtc gcggctgccg
     4381 ccgtccacca gcacgatctc gcagccgcgg tcgcgccaag cggcgagatg ggtgagcagt
     4441 tcgggcagcg tggccgcctc gtccaccatc ggcacgacga tggagagcga aaccgggcgc
     4501 ccctccatgt ccgacaccgc cgcactcacg ccttcagcgc cccgccgcag ctcgaaccgc
     4561 tgcccgcggt gcagccgaag cagtgatcgg cgacgcggat cggtgcgccg gcgagatctc
     4621 ccgcgagcag gtcgcgcaga tgcagtcgtc ccgggcgcgc cgccgcgtcg ccgtcgtcgt
     4681 gcccctgcgc ggacgtgagc gccccttccg cccccagcgg cagtccgagc tgctggttga
     4741 agtcgcagtc gtacagccgc ccctgccagt ccacgctgac caaggtgcgg cacatcaccc
     4801 cgccgagatt ctcgtcgcgg tggctggccc gcagcagcgc catgtaggcg ttgaactgcc
     4861 ccttcgagat cagcatcgag ccgaagcgct ggatcggcat gttggcgagc gcgaacaact
     4921 ggttgaagac gatgccgaac tgcgccgcga gctcgcgccg gtagtcggcc tcgagccggg
     4981 cctggtcggg cggcagcacc ggcccctgcg ggttgtacac caggttcagc acccggccgc
     5041 tgccggcctg gccatagccg agcgcattca ggcgcagcag gccggcgatg ctgcgctcga
     5101 acacgccctt gccgcgctgg cggtcgacgt tgtccgcggc gtagcagggc agcgaggcca
     5161 ccacctcgac gccgtgctcg gcgaggaatt cggcgagatc ctcctgcccc ggctcggaca
     5221 ggatggtgag gttgcagcgg tcgatcacgc gcaccccgag cgcccgcgcc tcccgcacca
     5281 gcgcacggaa gcccggatgc agctcgggcg cgccgccggt gaggtcgagc gttccgatcg
     5341 cgcgcgcggc gagcacgcgc gggatcagcg ccagcgtgtc ggcgtccatc atctcggtcc
     5401 ggccggggcc ggcgttgacg tggcaatgca cgcagctctg gttgcagcga tagccgaggt
     5461 tcacctgcag cgtggtgatc gcctcgcggc gcagcggcgg aaagcggtgc gattcgagca
     5521 gcgggagggt ggcatgcatg cggagtgtct ccatcgggaa agtcgattct ttcggcacct
     5581 gcggaatccg cggcagccgg cacggtcttt tgtcgtacct gcctcggaaa agcttacgct
     5641 cgggaagttc gaaccgttcc cctcgtccgt ccccccctca cccccccatc cgcgcctcct
     5701 tgtccgcccg aaaccatgaa gcgactcctc ctcgtcggcg gcggccacgc ccagctcgcg
     5761 gtcctccagg ccctcgcccg acggcgtccg cacggggtgg aggcggtgct gctgacgccc
     5821 tcgccctggc aatggtactc ggggatgctg cccggctgga tggccggcca ttaccgcgcc
     5881 gaggactgtc gcatcgacct gcgcgcgctc gccgacgcag ccggtgtgcg gctctgcctc
     5941 gcctccgcga tcgcgctcga cgcagaacgt cgcatgctgc tggacgacac cggcaccacg
     6001 cacgacttcg acctgctcgc gctcgacgtc ggcagcctcg tcgacacgcg cgcgctcgac
     6061 gccctgccga cggacgcctt gctggcggtg aagccgttgg aagccttcat cgccgcctgg
     6121 ccgcagcggc tggccatggc acgcgccggg cagctgcgcc ggctcgccgt ggtgggcggc
     6181 ggcgcggcgg gggtggagct cgtgctcgcc gccgcggagg cctttcgccg tgccgactgc
     6241 gcggtggcgc tcgcgctcgt cgcgccaccc ggcgggctgc tcgccggcca tgccgaaggt
     6301 gcgcggtggc gcgtgctgcg ccgtctggcc gcggccggca tcgcgctcca cccggctcgt
     6361 gcacggggca cgcccgcggg gctcgagctt gtggacgccg ggcgtgacgc gccgacaacg
     6421 ctgcccgcgg actgcgtgat cgccgccacc ggcgcacacg ccccgccctg gacgcaaagc
     6481 agcggcctgg cctgcgacgc cgcgggctgg gtcatggtcg acgccggcca tcgcagcgtc
     6541 tcgcatcctt tcatccacgc cgccggcgac gtgtgcaccc gcgcggatcc cggcttcgcg
     6601 cgctcgggcg tgcacgcggt ccatgccggc ccggtgctcg ccgccaacct gctcgccgca
     6661 ctggcgggcg gcccggcgag cgcgccggtg cgcagctacc ggccgcgccc gcgttcgctc
     6721 tacctgatcg caagcggtcc gcgccacgcc atcgcctcgt ggggacgctg gtccgccgag
     6781 gggcgctggg tgtggcgctg gaaggaccgc atcgaccgag gattcattgc acgtcaccgg
     6841 cgcgcagcca tcgtgccggt gtaagaatcc gcggagcaca tgcgacaaag cccgaagctc
     6901 ccttctcact cccttattcc ccggggcccc accatgcacg aactcgtcca ggactactac
     6961 ggccgcaccc tgcaatcctc cgccgacctc aggacctcgg cctgctgcga tgccagcgcc
     7021 ccgccgccgg cgctcaggcc ggtgctcggg cgcatccacc ccgaagtgct cgcgcgctac
     7081 tacggctgcg gcctggtgag cccgcccctg ctcgaaggct gccgggtgct cgacctcggc
     7141 tgcggttcgg ggcgcgacgt gtatgcgctc gcccagctcg tcggcgagcg tggcgaggtc
     7201 gtcggcgtgg acatgaccgc ggagcagctc gccgtcgcgc gccgccacga ggactaccac
     7261 cgcgaggcct tcggcttcgc gcgcagcaac gtgcgcttcc tcgagggcta tatcgagcgc
     7321 ctcggcgagc tcggcctgga ggacgccagc ttcgacgtga tcgtgtcgaa ctgcgtgatc
     7381 aacctgtgcc cggacaaggc cgcggtgctg cgcgaggtct tccgcctgct tcgccggggt
     7441 ggcgagttct acttctcgga cgtgtatgcc gaccgccgcg tgcccgccgc cgtgcgcgac
     7501 gatccggtgc tgtacggcga atgcctgggc ggcgcgctgt actggaacga cttcctcgcg
     7561 ctcgcccgcg ccgcaggctt cgccgacccg cgcctcgtgg aggaccggcc gctcgcggcg
     7621 accgaccccg cgctggccgc gaagctgggc gacgtccgct tcttctcggc gacctggcgg
     7681 ctgttccgga tagacggact ggagagcgcc tgcgaggact tcggccaggc ggtggtgtat
     7741 cgcggcacgc tgcccgagca agccacgcgc ttcgtgctcg acaagcggca cggcttcgac
     7801 gccggccggg tcgtaccggt gagcggcaac acctggcgca tgctgcatga cagccggctg
     7861 cggccgcact tcgacttcat cggcagcttc gagcgccatt acggccgctt caccgcagag
     7921 ggcccggcga tgcccttcga cacggcggcg cgcggcaccg tcacggacaa cccggcgccg
     7981 acgacaatgc cggcgccggt ccccgcgacc ctcggcagcg cggcgcgcaa atcctgctgc
     8041 tgatcgggcc acgacggctc cgcacggctc acccgctccc gggacgaaga tgaagaaacc
     8101 ccttctgctg ctgctcgtgc tcgcgctcgt cgccggcttc ttcgccagcg ggctgcaccg
     8161 ccagctcgac ctcgacaccc tcaaggccgg catggccggg ttcgctgcct ggcgcgaggc
     8221 ctcgccggtc ctggtcgccg ccctctattt cgccgcctac gtggcggtga ccgcgctatc
     8281 gctgcccggc gcggcggtga tgaccctggc cggcggggca ctgttcgggc tgggctgggg
     8341 gctgctgatc gtgtccttcg cgtcgaccat cggcgccacg ctcgccttcc tggtctcgcg
     8401 ccacctgctg cgcgacagcg tgcacgcgcg cttcggcgcg cgcctgcgcg cgatcgacga
     8461 gggcatcgcg cgcgacggcg cgttctacct gttctcgctg cgcctggtgc cggccttccc
     8521 cttcttcctg atcaacctgc tgatggggct caccccgatc cgcacgcgca ccttctactg
     8581 ggtgagccag ctcggcatgc tgcccggcac gctggtgtat gtgaacgcgg gcaccgagct
     8641 cggcgcggtg gacagcctgg ccggcgtgct gtctccgggc ctcgtcgcct ccttcgtgct
     8701 gctcggcctc ttcccgctgc tcgcacgctg gatggtcgag cgcgtgcagg cgcgccgggt
     8761 gtatgcgggg tggcagcgcc cggcacgctt cgagcgcaac ctggtggtga tcggcgccgg
     8821 cgccgccggg ctggtgacgt cctacatcgc ggcggcggtg aaggcgaagg tgaccctggt
     8881 cgaggccgga cgcatgggcg gcgactgcct caacaccggc tgcgtgccga gcaaggcgct
     8941 gatccgcagc gccaggctgg cccaccagat ccgccacgcc gaccgctacg gcctggagcc
     9001 cggcgagccg gagatcgact tccgcaggct gatggcgcgc gtgcgcgcgg taatccgcaa
     9061 gatcgagccg cacgacagcg tggagcgcta cagcgcgctc ggcgtcgagg tgctgcaggg
     9121 gcgcgcgcgc atcgtcgacc catggacggt ggaggtggcg ctgaacgagg gcggcacgcg
     9181 ccggctcagc acccgcagca tcgtgatcgc caccggcgcc cgcccgaccg tgcccgacct
     9241 gcccgggctg gaggcggtgg gctacctcac cagcgacacg gtatgggacg ccttcgccgc
     9301 gctcgacgcg ccgccgcgcc ggctgctggt gctcggcggc gggccgatcg gctgcgagct
     9361 ggcgcaggcg tgcgcgcgcc tcggctcgca ggtggtgctg gtggggcgcg cgccgcagct
     9421 cctgccgcgc gaggacgccg atgtcggcgc cttcgcccgc gccgcgctgg aggcggacgg
     9481 tgtggaggtg ctgaccggag tcgcggcatt gcgctgcgag caggaaacgg cagcgggcgg
     9541 cacagacaaa ttccttgtcg tcgccgaccc cgccagcggc ggcgagcgcc gcatcgcctt
     9601 cgacaccctg ctgtgcgcgg tcggccgcag cgcccgcctg gagggcttcg gcctggaggc
     9661 gctcggcatc ccgaccggcg cgacggtggc gaccgacgac ttcctgcaga cgcgctaccc
     9721 caacatcctc gccgccggcg acgtcgccgg ccccctgcaa ttcacccacg tcgccgcgca
     9781 ccaggcctgg cacgcggcgg tcaatgcgct cttcggccac ctgcgccgtt tccgcgtcga
     9841 ctaccgctgc gtgccccaca ccaccttcac cgaccccgag gtcgcccgcg tcgggctcaa
     9901 cgagaccgag gcgcacgcac gcggcatcgc gttcgagttc acccgctacg atctggccga
     9961 gctcgaccgc gcaatcaccg atggcgccga ccgcggcttc gtcaaggtgc tgaccgtgcc
    10021 cggcaaggac cgcatcctcg gcgccaccat cgtcggcgag cacgccggcg agttgctcgc
    10081 cgagttcgtg ctggcgatga agcacggtct gggcctgaac aagctcctcg gcacgatcca
    10141 cgcctacccg accttcgccg aggccaacaa gtcggtcgcc ggcgaatgga agcgcgcgca
    10201 cgcgccgcag cgcgtgctgc gctggctgga acgccaccac gcctggcggc ggggctgacg
    10261 cgccgcgcgc atcaggcgcg ctggtagcgc gtccggctcg acagcaccag gccgacaagg
    10321 gaggcgagcc cggtgatcac ccaggtccat tgccagccgc cgacagcgcc cgccacccag
    10381 gccaccagcg gcgggccgag gaactggccg gccgaggagc actgctgcat ccagcccacg
    10441 gtggtcgaca ccgtgtcgtc gtcgggcgcc acatgcaccg cgagcgcgaa cagggtcgcc
    10501 gggataagcc cgccgatcgc cgagaacagc accacggcca ggtaacgcgc gaccggcaac
    10561 tctgccgcga gcggcgagaa ggcgacgaag gtggtcgccg ccatggcgag gaagccggcg
    10621 tacagcaggt ggcgcgcgca cacgccgcga tgcagcaggc ggccggcggc gacgttgccg
    10681 gtgacgttcg cgaggcaggc gaaggcggtc agcagcccgg ccacctggcc cgacagtccg
    10741 gcctgggcgt agatcgacgg caggaatccg atcaccgcca gccactggct ggagtacatg
    10801 gagaaggtga tcgccacccg ccacggcccc gcgctgccga gcgtggcgcg caggcgtgcg
    10861 ccccagcgcg cacgtgccgc gaccgccgcc ccgacggggg tgcccgccgc ggcacggcgc
    10921 gcgtggtcgg atggcacgcg caccgccagc cacgccgcca ccgcagccga ggccatcgcc
    10981 agcagccccc acagcccctg ccagcccatc acccccatca gcgccggccc accagccagc
    11041 accagcgagg ttcccgtggc catgtaggtg ccccacaggc ccaggtgcag cgccagccgg
    11101 ctggggggca cgagctggcg gatcagactg ggcaccggca gcaccacgag caggaagccc
    11161 agcccctcga tcgcgcgcaa gaccagcagc tcgccgggct gggtcgccca cgcgccggcg
    11221 aggctggcgg aggcgagcac gccctggccg agcagcacgc tgcggcgcag gccgaagccg
    11281 tctgcggcca ggccgatgaa gacgccgccg agcatgcccg ccatctgcac catggagagc
    11341 aggaagccgg cctcgaccag gctcacgccg agcgcatcgc gcagcaccgg gatcgccggc
    11401 gggatcttgc cgatgtgcag cgcggcgacc atgccggcga tgatgacgac gaaggcgggc
    11461 gggacgcccg cagccgcgga cggcgcgtgc ccgcccatgt tccgggaagc ttctgtgcgg
    11521 ctcatggtct cctctcagcc tctcgatggg cgacgagagc atagccgcga tcgcgccggc
    11581 gtgcgcgcgg gcgagcccgc cgcgttcgcg gcttggtggt ccgccccccg ccgtgtacat
    11641 ttgcgcccac cccgcttccg cgcccgcgcg ccccgcccgc catgaccacc ccgcaccccg
    11701 aaatccgccc cggccagtcg gtcgagctgc tcaagcagct ccacatcctg acccgcgacg
    11761 gcaagctcaa ccaggacagc cggcgcaagc tcaagcaggt ctaccacctc taccagttca
    11821 tcgaaccgct gctcgccgag gccctggccg agcgccccga catcgagctg gtcgaccacg
    11881 gcgccggcaa gtcctacctg ggcttcatcc tctacgacct gttcttcaag gcgcacgccc
    11941 ccggcggccg catccacggc atcgagacgc gcgacgagct ggtgacgagc tcgcgccggc
    12001 tcgccgacaa gctcggcttc tcgtccggga tgcgcttcca gaacctcagc gtggcggact
    12061 cgatcacctc ggaccgcctg cccggacgca tcgacctcgt caccgcgctg cacgcctgca
    12121 acaccgccac cgacgacgcc atccgcttcg cgctgaagaa gggcgcccgc gccatcgtgc
    12181 tggtgccctg ctgccaggcc gaggtcgccg cggcgctgcg ccggaacaag caacaggcgc
    12241 tgcgcggccc gatcgcggag ctgtggcgcc accccatcca cacccgcgag ttcggcagcc
    12301 acgtcaccaa cgtgctgcgc tgcctgcagc tcgaggccca cggctacgag gtcacggtca
    12361 ccgagctcgt gggctgggag cactcgatga agaacgagct gatcatcgcc cgccgccagc
    12421 ccgagcgccc gcgccggcgc gcggccgaac gcctcgccgc gctgctcggg atgctcggtc
    12481 tggaagaact ttccgagcgt ttcttcacga gctgacctgt ccgcgccgac accgccgatc
    12541 catgcctttg tgctgcgatc gaactgctag aatcggggag tccgccacgc aaggtggaca
    12601 ggagacaggg cgccgcgccc ggacggaacc ggaaagtcga ggaggacgga tgcgggcgca
    12661 tccggaacgg acatggccga aatcgaatca ccgctggcat tcctgctgac cacgccgtac
    12721 gcgagccgct atccgcgcaa gctcgtcgag ggctttcccc atgtcgcccg ccgcatcgcg
    12781 gagctctggg acgacaagga caagctgggc gaatacttca ccgagctcat ggtctcgaaa
    12841 cggccgaacc ggcgcggttt cccgcccgag gtgggcgcag agatcgttta tctgagcatg
    12901 gcctacgacc tccacggccc ggtgcgtccc accccgcaag cggcccccga gcccacgacg
    12961 gccgcgcgcg acgacgcctg ggactacgag cgcgcggtcg ccgagctcga gcgcctcgac
    13021 attccgatca cgatggcgca gttcgtgcgc gcgctggagg ctggcgacca gcacctgtgc
    13081 tcgctcttcc tgcatgccgg cttcgacatc gacggccgcg acgcgcgcca gtggacgccg
    13141 ctgatgatcg cctgcttcca tggccgcgag gcgctcgccc tcgagctgat ccggctcggc
    13201 gccagcgtgg atgcgacgga cgccgacggc tacacgccgc tgcactgggc ctcggtgaac
    13261 ggctaccaga aggtcggcga ggtgctcgtg cgccgccagg ccgaggtcaa tgccacgagc
    13321 aacgccggca tcacgccgct gctgcaggcc gcggcgcgcg gccacctggg cgtcgtgcgc
    13381 ctgctgctcg accgcaaggc caaggtcaat ctcgtcgccg cggacggctc cacggcactg
    13441 ctcaaggcgg tcgccaacgg acactgggag atcgtcaaca cgctgctcga cgccggcgcc
    13501 tccacgcagg cgacgatgaa gaacggcacc accctggtcg acatcgccgc acgttcgaag
    13561 gacgagcgca tccgcgaacg catcgccatc gccgcgcgca tggaggcgcg cggcgatgcg
    13621 ccgatccagc gcgacgagcc gccaccgctc accggcacga tctactgacg gcggggcgcg
    13681 aacgccgcct tgccgggacc gtccgtgcga tccgtccgcc cgctcgcgac tcgcgtcgcg
    13741 ccggggcgct gcggataccg tgccggttga tcgtgggagc gacgcgagtc gcgatcgctg
    13801 cggtggatga acggcggcga cggcgcggag gcgggataca atcacgctcc gctggaccgc
    13861 ctcattgccg atgacgaaac attccacccc gcccgcgcca ccggccctgc tcccttcgct
    13921 gcggacggat gcgggcggcc catggcggtg atgccctccg cagcgctggc ggtgctggtg
    13981 gtggcgctga cctcgctcgg gccgctctcc accgacttct acctgcccgc cctgcccgcg
    14041 atcgcgcgtg ccctgcacac cgacagcgcc ggcgtgcagc tcaccctgtc ggtctacctg
    14101 ctcggcttcg gcgccgggca gttgctcgtc ggcccactct cggatcgctg cggacggcgg
    14161 ccggtgatgc tgtggggcat gctggtgttc ctgctctcca cgctggtgtg cgtgttcgcg
    14221 gaatcgctcg ccgtgctggt gggcgcgcgc ctggtgcagg ccctcggcgc ctgcgcaggg
    14281 ccggtgctcg gccgtgccgt ggtgcgcgac ctctacggcc ccgccgaatc ggcgcgcgta
    14341 ctctcgcacg tgtccaccgc caccgcgctc gcaccgctgc tcgcgccgct gttcggcggc
    14401 tggctcaccg ccgcctgggg ctggcgcgcg accttcgtcg cgctcgcgat ctacgccatg
    14461 gtcctgatgc tcgccgtggc cgcgctgctg aaggagacca accgccaccc cgatgcccat
    14521 gcgatgcgcc cggcccggat gctggccaac taccgtacgc tgctcgccga ccccgcctac
    14581 cgcggcgcgc tgctgatcgg ctgcggcgcc ttcgccgcgc tgttcgcctt catctccggc
    14641 tcgcccttcg tgttcatcga gcacttcggc atgtcgccgc agcagatggg cctggccttc
    14701 gggctcaacg tcaccggctt catgatcggc tcgacgctgt cggcgcggca ctcgcgccgc
    14761 ctcggcccgg cgcgcctgat ccgctacggc gtgtggatcg gcgcggcgtg cggcgtgctg
    14821 ctcgccgcgc tcgccttcgc cagcccgcag cacccggcgg cggtgatgct gccgatgtgg
    14881 ggcgtcaccg cggcgatcgg gctgatcctg cccaacgcca ccgcgctcgg actcgccggc
    14941 tatcccaaga tggccggtgc cgcggcctcg ctgatgggtt tcgtgcagat gggcctgggc
    15001 gccggggcgg gcatgctggt cgggcacggc gtgcagcacg gcaccgcggc gctcggtctc
    15061 accgtcgcgg ccggcatgct gttcgccctc gccgcatgga gccgctcacc cgcgcgctga
    15121 acgcgggcgg cgttcacaag aacgcacggc cggcctccac cagcccggcg atgtccgccg
    15181 cgagttcgtc caggcgcgcc gccacgtcgg gctcgtcctg cgcgattgcc ttctccagcg
    15241 ctgcggccgc ctgcatgatc gcgagcgcgc agatgaagcc ggcgttgctg cgcagcgtgt
    15301 gcatgcggcg cgccgccgat tcgcgctcgc cgcgtgcgag atcggcgcgg gtcgccgcca
    15361 ccaccccggc gttgtcctcg atgaagagcc cgagcaggcc gatgaacatg tcgcgatcct
    15421 tgccgaggcg ctgcatcgca cgctcgcggt cgatgccggg cagcgcgggg aaatcgtcgc
    15481 cagccgcggc aggcggcgct gctgcgctct gcgccggcga cgcgggcggg ggcacgggcg
    15541 cggcggccgg catgggtgcg gcagcgaccg cgtgcgcagg ccgatcaccc gcgccggccg
    15601 cgggcgtcgc ttcggccagg cccgcagccg gctgcggcat tacccagcgc atcagcagct
    15661 gcgtcatctg ctccaggtcc atcggcttgg gcagcacgtc gtcggcgccg gcggcgcgcg
    15721 cggcggcctg ctgatcctcg cccacgccgg cggtgaaggc gatcacgggc agcgcggcga
    15781 ggccgagctc gtcgcggatg cggcgggtgg cggagaggcc gtcgagcacc ggcatctgca
    15841 cgtccatcag caccgcgtcg aaggcctgcg gccggccacg cagcaactcg atggcctgct
    15901 ggccgtcggc ggcgagggtg gcgcacgcgc cctccagcgc cagcgcgcgt tgcaccagat
    15961 cgcggttcat cgcgctgtcg tccaccacca gcacctgcat gccggccagc cgcggcccgg
    16021 ccgcgcgatc gccttccggc gccgcgacca ctccgacgct cgccggctcg ccgccggcaa
    16081 cccgctccag cggcagctcg aaccagaagg tgctgcccag cccgggctgg ctatgcacac
    16141 cgatcgcgcc ccccatcagc tcgaccaggc gcttgcagat cgacaggccc agcccggtgc
    16201 cgccgaagcg ccgcgcgatg ccggcatcgg cctgggtgaa gggggcgaac aggcgcgcct
    16261 gcgcctcggg cgcgatgccg atgccggtgt cgcgcacctc cgcacgcagg cgcagtcgca
    16321 cctcccccac ctcgtcggcg cgcacgcgca gggaaacctc gccacgctcg gtgaacttga
    16381 tcgcgttgct gaccaggttg atgaggatct gctcgatgcg cagcccatcg ccgcgcagct
    16441 gcccgggcgg cagcgccggc ggctcgatgc gcagcgccag ccccttcgca cgcgcggcct
    16501 ggcccatcag gctgtcgaga ttggcgagca gcgcgcgcag gtcgaagggc cgcggctcga
    16561 tgcgcagctg gccggcctcg atcttggaga ggtcgagcac gtcgtcgagg atcgccagca
    16621 gcgaagcgcc ggcgccgcgg atgcgcccga ccatgtcgcg ctgcgcaggc gcgagcggct
    16681 cgcgctcgag gacctgggcg aggccgagca ccgcgttcat cggcgtgcgg atctcgtggc
    16741 tcatgtgggc gaggaactcg ctcttggcgg cgttggccgc ggcagccgcg gccgaggcct
    16801 cgcgcaggcg ctcctccatc tcgatgcgcg cggagatgtc ctcgacgatg ccgaggtagc
    16861 gcggtgcgtc ctccgaggcc acctgcaccg gcgcgaaggc caggctcacc cacaccggcg
    16921 tgccgtccgg gcgcaggtag cgcttgttga ggcggtagcc ggcgatgcgc ccggcgtcga
    16981 ggcggccgag ctggcgcagg ctctcggcga cgtcatccgg atgagtgagc gcgatcgggt
    17041 cgacgccgac cagctcgccg acgctgcggc cgacgatcgc ggcgaagcgc gggttggcct
    17101 cggtgatccg ccgtgtgcgc gggtcgagca gcaccacgcc cagcggcgcc tgctcgaaga
    17161 tcacccgaaa gcgcgcctcg gcgatctggc gggcctgcag ggtggcgcgc tcgcgctgct
    17221 cgcggtcgcg ccgccacacc aggtagccgc ccgagtacac cagcatgagg ccgagcgcca
    17281 tcaccacgcc catgccccag gtcagcgtgc ggatgcccga gtaggcctcg cgctcgtcga
    17341 tctcggcgag catcagccag ggcgtgccct cgaccgcggc ggcgtaggcc agtaccggca
    17401 cgccgcggta gtcgcgcccg ccggagagga tgccgcgctc gccgagcgcc gcgcgcgcgg
    17461 ccggcagatc gggagtggcg agcgggcgct cgagcgcgag cgccgcatcg gcctgatggc
    17521 gcagcggggt caggaagcgc acgccctcgc cctcgcgccg caccaggtaa gtctcggcgc
    17581 tgcgcgtggg caccggccag gcctcgacca tcggatacag cgcggtctcg gcctcccagg
    17641 tcaggtaggc cacaccgcgc gacggcgcgc ccaccggcgc gagcacgccg taatgcgtgc
    17701 gcccccgggc gtcctggtgc aggtcgacga gctcgacgcg cggctgccgc agcacgtcgg
    17761 cgatcagttc ggcatgggcc gagaggtcgg ggctgccgac cgcgagcgca ggcgcgccgt
    17821 cggcgtcgag cagggcgacg ccctgccagc cgcgcaggcg ggcgagctcc tcgacgagcg
    17881 cccgcatgcg cgcgaacgcc gcatcgtcgc ggcggccgcc gtccagccag gcctcgaaca
    17941 aggcctcgag ctgcgaatgg ccggtgaaga ccatccgcac atccacgcgc gcctcggcga
    18001 gccagccttc gatgtgctgg cgcttctgct cggcgatgac cgccagcgtg cgctgggtct
    18061 cgcggcggat ctcctcgctc agcgagcggt agccgaaggc acccacgccg cccaccagca
    18121 cggtgagggc gatgagcgga aaggcgaggc gcaggccggc atggcggccg cgggtggcgg
    18181 catcggcggc gggagcgtgg ttcatggcgt ggaggcgtgc gatcgcggcc gggccggtgc
    18241 acgcagacag aagaatgtcc gcattgttgc gctgcggcaa gcgcgctgca acccgcttcg
    18301 aggcgtctag ccgccgccgg gcagaaagag cgccgggtcg accatgctgc ggttgagcat
    18361 caccgaccag tgcaggtgcg ggccggtgac gcgcccggtg gcgcccaccg cgccgatgcg
    18421 tgcgccggcc ttcaccgtgt cgcccgcctt caccgcgatc gaggacaggt ggcaatacat
    18481 ggtgagcagg ccgcggccat ggtcgagcca taccgtgttg ccgttgaaga agtagtcgcc
    18541 ggtgtcgatc acccggcccg ccgcggcggc caccaccggg gtgcccgtgg gcgcggcgat
    18601 gtccatgccg ccatgcggct tgcgcggctg gtcgttgaac acccggcgca gcccgaagga
    18661 gcccgagcgc cgccccggta ccggctgcag catgcgcagg ctgccgggat cgagcgtgct
    18721 ccaggtggcg acgatctcgg ccgagcgcag gcgctcgcgc tcgtggcggg cgaggtcggc
    18781 gggcgacagg tcgaccttgc ccggcgccac cttcaggcgc tgctcggcgt agcgcaccgg
    18841 ccccaccgcg accggcaccc ggcgcggcgg ctcgccttcc gcgcgcacct ccaggtccac
    18901 gctgcccggc tcggcttcga gcgcaatgcc caccaccgcg gtccacccgc ccgggcgccc
    18961 ggcgaccagc accggcacgc cgtccaggct ggcctggggc gcgaccagtg cgggtccgag
    19021 ttcgaccagg gcgaccccgc ccgccaccgc gcgctcgcgc ggcagttcca gcgccgcgac
    19081 cggcagggca acgcaggccg ccgcagcggc cagaacgcgg gcaaggcgaa cgcgggcaag
    19141 gcgtatacgc cacccgcacg aagggaacga gcgcatcatc ggattcctgg attcgggggg
    19201 ccgtgccggc gcggtgccac gcctgcagca tgaagctgcg gacggtgtcg cgacgacgac
    19261 cgatgccgcg atgatagcgg cgaaaagcgc ggagatcgcc gatgcgcacg ctagaatcgc
    19321 gccctcgctt tttccgcccg cgatccgggc ttcatgacga aacgccgcca cccgcggctg
    19381 gagaccaaac atggaagcat tcgccaacca ggtcgtcgac ctgctcaacg gcctgctctg
    19441 gggcaagatc ctgatctggc tgctggtcgg caccggcatc tatttcaccc tgcgcctggg
    19501 cttcatccag ttgcgccact tcggccacac cttcacggtg ctgcgcggca gccgccagtc
    19561 cgacgcctcc ggcatctcgt ccttccaggc cctgtgcacc tcgctcgctg cgcgcgtggg
    19621 caccggcaac atcgccggcg tggcggtggc gatcacgctc ggcggcccgg gcgcgatctt
    19681 ctggatgtgg gtgatcgcgc tcgtgggcat ggccaccggc ttcgccgagg ccacgctcgc
    19741 gcagctcttc aagcagcgcg acgacaaggg gcagttccgc ggcggccccg cctactacat
    19801 ggagaaaggt ctcggccagc gctgggcggg cacgatgttc tcgatcttcc tgatcatcgc
    19861 cttcggcttc gccttcaact cggtgcagtc gaacacgatc gccggcgcga tggtcggcgc
    19921 cttcgagctc gacatgggca gcttcacgct gggcggcaac gaggtcgccg tcgcggcgct
    19981 ggtggtcggc gtggcggtca ccgtgctcac cgcggtgatc atcttcggcg gcctgcgctc
    20041 gatcgcgcgc ttctccgagc tcgccgtgcc cttcatggcg gtcggttacc tggtggtggc
    20101 ggtcggcatc ctcctcgtca acctcggcga ggtgccgggc gtgctggcga tgatcgtcaa
    20161 gagcgccttc ggcttccacg aagccgccgc cggcggcatc ggcgcggcca tcctcaacgg
    20221 cttcaagcgc ggcctgttct ccaacgaggc cggcatgggc tcggcgccca acgccgccgc
    20281 cgcggccacg ccctacccgc cgcacccggc ctcgcagggc tacgtgcaga tggccggcgt
    20341 gttcatcgac acgatcctga tctgcaccgc gtccgccgcc atcatcctgc tcgccggtcc
    20401 ggtcgaaggc accggcgtcg gcctggtgca gaacgccctg accagcgaag tgggcggctg
    20461 gggcaagtac ttcctcgccg tcgtggtgct gttcttcgcc ttcacctcga tcgtcgccaa
    20521 ctacttctac gccgagaact gcctggtgtt catcgagcac aaccaccccg cgggcctgct
    20581 gatcttccgc ctgatcgtgc tggccatggt gatgttcggc gcgatcggct cgctgccctt
    20641 cgtgtggaac ttcgccgacg tggcgatggg cctgatggcg atcaccaacc tcgtcgccat
    20701 cctgttgctc tcgggcctgg tggtgaagct ggcgaaggac tacaacgccc agcgcgccgc
    20761 cggcaagctg cccaccttcg acgcccgcgc gtacccagag atccgcagca agctcacgcc
    20821 gggcatctgg gactgagccc ggccacgccc cgagcggcgc caccccggcc ggatgccagg
    20881 cgcgtcccgg agcgggcgcg ccgcgcagcc gccgatccga tgcacagcct cctcgagttc
    20941 ccgagcccct tccccgccga gcccggcgtg gtgcgcctgc tcgagccgcc cgggagcgac
    21001 gccgccgcgc tcgccgcccg cctcgtcgca ggcgactacg gcaagccctt cgtgctcgag
    21061 aatggcggcc tgcgcgcact gcacttcagc ctcggctacg tgcagagcgt gatggacatc
    21121 gccaatccca gcgcgctcgt gctcgcctat gcacgcgcga tgatggcctt cctgctgttc
    21181 aatccgcgcc cacgccggat cggcatgatc ggcctcggcg ggggctcgct ggccaacttc
    21241 tgccaccgcc atctggcggc ggccgagctg accgtggtgg agatcgacgc cgacgtgatc
    21301 gccctgcgcc gacacttcgg cgtgcccgcc gacgacgcgc gctttcgcgt cctccacgcc
    21361 gacggcgtgc gctggctggg cgagacgcag acgcgcagcg acgtgctgct gatcgacgcc
    21421 ttcggcgccg agggcgtcgc ccacaccctg ctcgacaccg atttccacgc caacgccttc
    21481 cgtcacctgg gcgggcacgg tgtgctggcc atgaacctcg ccggcgcacg cgaatccttc
    21541 gccccccacg tcgcccgcct gctcgagatc ttcgacgaac gcgtgatcgc gataccggtg
    21601 cgcgaggacg gcaaccacat cctgttcgcc ttccgcgatc ccgccttcac gccgcgctgg
    21661 aactggatgc gcagccaagc ccacgccctg caggcgcgca ccggactgga ctttcccaat
    21721 ctcgcccgcg cgctcgaagc cggcgaacgt cagggccatg cctggcggct cgcacgctga
    21781 gcgggcggct gccgtcggct cgacgccgac tcagcgccgg ttgttgaacg ggtagccgta
    21841 caggcgcagc agctggcgga aggcctccat gctgaccgcg gggtcgtagc tggcgaacag
    21901 cttttgctgg cgctcgtgca gcgccgcggg ctgtgccgac agcagcatct cgcgctcgtc
    21961 gagaatgtcg tcgcgcatcc gcggactcac gaacaggaag gggatcggcc ggtacaggcc
    22021 gtcgtcggac gaaaggtgat aacgggggtg ggcgatctgg atgttctcgg cgagatgact
    22081 cttcatggct ccgtcgtacg gtcgaaatcg atgtccgtcc cgcaccacgc gggctcggaa
    22141 agctgtcgac gcgggcggca ggccggcgcg tccgtctgaa cgaccatgga agcggaaaaa
    22201 agatcccgcc gcacacgctg cgtgcgcggc ggcagcggcg tggatccgct caatgcgagc
    22261 cggcgaaggg gtagcgatag agctcgagca gctcggtgaa gctgcgcgcc cccgccaccg
    22321 ggtcgtagcg atcgaagagc ttctgctggc gtgcgcgcag ggccgggggc agggaagcga
    22381 cgagcagcga gcgctcgttc aggatgtcgt cgcgcatccg ctcggtcacg aacaggaagg
    22441 cgatcggtgc atagagtccg tcgcgggtgg gcaggtgaaa acgcgggctg gcgatgcgga
    22501 tgtcttcggc gagatgactt ttcatggttc caggctcgca ggcagtgggt ctccgtcctt
    22561 cctggaatca tccctcatac gccccgcaac gcgtagcatg aaagcgcggt gtggccgcaa
    22621 tccatcacgc tcgcacgcaa aaggcgagcg cggatttccg tcagcagcgc gctaacgcgc
    22681 cggcttcggc gcgaccgccg ccgccccgca gcccgcaccg agccagcgcg catccgtctc
    22741 gagatcgagg tcgccgcgca ccgaggcgag cttcacgcgg gtcgtgaaat gctccgggcc
    22801 ttcgacgcgc gcgtggaaat cgccctcggc gtccggatcg gtgcagcgca ggctgccgcg
    22861 gatggtgttg ccgctacgct cgacccgcgt cagcgtgcag ttgccctcga ccttgccggc
    22921 gaagacgttg tcctggcgcg cctgcgcggg tgtgatgcag ctgcgcacgc tgccgtccgc
    22981 gcccaggctc accccgcgct gctgcatctg ctgctccagc atgcgccggg tgtcggccgg
    23041 caggcccttg atgtgttgct gcagcatgcc catctgcgcc gccatgtcgg gcaggccggc
    23101 caagctcagg cgggtggagc ggaactccca caggccgggg cggaggtcgt ccagcgcgtg
    23161 cgcggccggc acggcgcagg tcaggacaag agcgagcgcg gcagcgcgga tcggggtcat
    23221 cggggcggtc ctcgtggttc ggcgtgcggg cgcggaagcg cgcccgacat cgacgcctgc
    23281 attatcgcag gccgggggcg ggcgacgacg cctcgcagcc ccccggatcg tgagcggatc
    23341 ttgcggcggc acgaaggagc gcggtgcggc ggacagcggc ggaggagccc ggcgtggcgg
    23401 gtatcggcac gccatgcatc gcgacttgcg tcgcgcctac aggacccggc ccggcccgat
    23461 ccgtcccgcc agcgcaagca gcgccgcgtg atccacgagc cccttcacga agcgcgcggg
    23521 gatccccgcc agaccgacct gcgcccccac cagcgcaccg gtcagcatgg tgcgcgcgag
    23581 gttctgcccg ccgccattga gcgcatgcag caccgccgac tcgaagtcgt cggcgaagcg
    23641 cgccgcgagg taatacgccg ccggaaactg gtgatacacc gcgcagggca tgccatacac
    23701 cagcgcgacc ttccacgccg gctcgatgcg cacgtccggg tcgtgcgccg cgcgcgcgat
    23761 cgacgactgc gtgagcagcg catccggcga cgggaagcgg ccggcggtcg gggcctcggc
    23821 ggcaccgggg cgcggcgcct ggaggtttcc cgcggtcacg gtgtgaaagg gcagcgcgcc
    23881 ggacttcacc cgcgccatca gcttggccga gatcgccgcg tcgaacttgt ggccctcgac
    23941 cagcatgccg agcaccgcgc cgaaggcgac ggtcatcgcc accacggtgc cgtcggtctg
    24001 ggtcagcacc gtgttgcggg tgacggcgtc ggcgagcgcg gcggggtcga gggcgtagcg
    24061 caccgcgatc gccagcgtgc gctcggcggc ctcggtggtg tcggcgttgc cggccacccg
    24121 gccccagggc aggccctgcc ggatgcgctt gcgccaggcc tcgcggatcg actggctggt
    24181 gtagccgccg ggcccctgca tcggcgtgcc gtcgagtcgt ggaaagaaat cctcgtccat
    24241 gcggcggcag aagtcggcct cgtcgtagcc gccgcattcg gccagcgact ccatcgtgag
    24301 cctcagcagc acgccggcct gcgaggattc gcccgccttc atgcccgcat ggtagtggtc
    24361 gggcatgggc gcggtatagc cggtgatcca cggcccgtag cgcgcgcgca gctcgtcgag
    24421 gtcgtagtac cagtgcgggc cgagcgccag cgcgtcgccg atgaaggctc cgagcagggc
    24481 accgagggca cggtcccgca gggcggcggg cccgtcgatg cccgcgacgg aaaccgggtc
    24541 ggcagacatc aggggccgat cgccacggtg tcgagcgcct tccaggtccg cggaccgacg
    24601 atgccgtccg gcaccaggcc gtgcgcgcgc tggaagcgcc gcatcgccgc ctcggtcagg
    24661 gggccgaagg cgccatccac ctgctgtgcg ccgaagccga gcttgcgctg gacttccttc
    24721 accagaaaga tcggattgcc cgtcgcgccg cgccggatgg tgggtcgatc ctccgcatcc
    24781 ttgcgcggga tcgagggcac gggcgcagcc gcgccgctca tgagggccgc gacctcgctg
    24841 cggaaacgct ccatgtcgag cgtgtgcggg tcgctcttgc gccctttcgg cagcgcatat
    24901 tccttgtgac cgcagcacat cccggcatcc tggccgagat gcgccaggat ggcggccacg
    24961 ccgcgacgat aggcgtcgag ctgcacctcg ggccagggtt cgaacccggg tttggtcgga
    25021 tcaccgattc cggtgctctc ggcctcgatg ccgatgaagc tgccgttgcc gtcagtgatg
    25081 cccttccact cgccccgccc cgcgtgattg gcccttcccg cggcgatcac gtagaacgtg
    25141 ccgtcccgcc cgagaccgag ctgcgccagc ggccccgcca gatcggcacg cccgttcttg
    25201 agtaccccga ggctgggcat gtttccgctc cgcggaccgg cggtgtgatg gcagatcacg
    25261 cccttgacga cacccatttc gctccgtccg cggttcggcc agccatcgac ctccgcgacc
    25321 ttcaagccgg cgtccctcag aacgaccggc aaccatgtca gctggaatgg catgtcaggc
    25381 taccccccct tcggcggcgg gaaggtcttc gtcgggcgtc gcctcggtct cctggaccgg
    25441 gcggtcgcac acgtcgatct cctgctcgtc gggttcgcgt ttttcggtcg ttccgttcat
    25501 cttgcagccc tccttcctgt cctgggtgcg cccgcaggac ggtgacgccc atgcgggata
    25561 cccttcaata taggccgtcg atgcgccccg acaagcggat tccaggcccg atcaggtcgt
    25621 ccattggtcg tggatgaccc ccaccagcag ccagcgtccg tccacctgct cgaagaccag
    25681 gcgcaaggcg ctccagtcga aggccgcggc cccgccaccc ggcgcgggct cgacgtagta
    25741 ctccacccgt gttccctgcg gatacgcttg ggcagcgttg tcggcagtgt tgccgcgctg
    25801 ctggtcgaca ttcacaccga tcctgcccgc cccgcgatag tcgcggtcga gcacgaagcg
    25861 ctcgacatac tgcgcggccg tcaaggcgat cggctcgccg ctgccgtcgg catggcccca
    25921 cacgtggacc gtggcgtcgt cccagaagtg gcgcagctgt gcgggcagga agacgcgatc
    25981 ctcctcgaca tcgacgtagg catagggcga gaagcgcacg cccttgtgcg gatgcgccct
    26041 gcccgcgagg cgttcgccgt cgcgtcgcac aaaggcctcc acgacctcgt tcgcgaccag
    26101 gacgatctcc tcgaccgcag tgcgggccgg agccggcgct ggcgccggca cgccgcgcct
    26161 ttcgcccgcg tacaggccgg ccacgagcag cagcacggcc agcgccacca tccacagcgt
    26221 cttcgcgcgc ttcatcgacc cgccccctca ggccgtcaac tcgccgatca gggcggggtc
    26281 cgcgatgccg gtggccgcca gcccgtgctg cgcctgccag gccttgagca ggttgaagct
    26341 ggtccgcccg aagatgccat cggcgaggat gtccacgccg cggtcggaca ggccgagctg
    26401 cagcaggcgg acatccagcc cgcgcgccag cggggtcgcc agcgtcaagg ggcgcgaacc
    26461 cggctgcggc ccgtcgtagc agccgggcgg caaggcggcg agcgtcacgc tcgagatctc
    26521 cctgccgcgc accacgagcg gcatctcgag cccccagaag ccctggtcga tgaggcgtcc
    26581 gaacgcttcc atgcgataca ccgtctggcg caggtcggcg cgcgcgtggc cctccagcca
    26641 ggctcgtcgc gtgcgcacat aggcgtcgat ccaggcctgc tcgcccaccg ccgcgggctc
    26701 gccgaccgcg cgcgtggtgc ggtcgcgcat cgccagccag gagccgtgca cggcgctgtc
    26761 gtacaccacc gccacgccga gcggggtgtg cacgcccaag cttgcggccg cgcgcagcgc
    26821 aggctcccag taggtccgat cgaaaaacgc atcctgcgta tcgcgcatca ccggatcgtc
    26881 ggcggtcgca cgcagcacgt tgtgcaggcg gggatcgtcg tcgaggctct cgtcgatggc
    26941 caggaagcgc ggcaggtagg acgccagccg gccggcgaag cgtgccccgg ggttggcgca
    27001 gtattgctgc agcagcttgg cgaggttgcc cgagccgagc gtggtctgcg atcggccgaa
    27061 ggtcaggtgg ccggtatcgc ccgggatcag cgtcaccttc ccgtactggc ccagcacctc
    27121 cccggtctcg aacaggttga cgatcgcctt ggcgaccgcc gcctgggtgg cggtgacgcc
    27181 accggcgacc gcccccctcc ccgcgcgcgc cgctgcggcg ggggctgcga ggcttgcccc
    27241 ggccgccggc gtggcgctgc gatcctcgaa gcgcagcacg cagaacccgg gccgctcggg
    27301 atggggatcg cagctggtga cccggaagcc gagctgctcg acgtcgagcg tcttctcggc
    27361 gcaatccgtg cagggaatga tgcgttccat ggctcgctcc ttcgggttcg gggatcaacg
    27421 cccggcaagg ccgcacacgt actggcggtg cagggacaga cgcgtcgtgg cgtccgcgga
    27481 atcggcgtcc agcagcgcca ggtagcgggt atagcgctcg cgcgcggcat ccgggcccag
    27541 gctggcgaac gcgccggcac cggcttcctg gtccaggacc cccagcgcct cggcgttccg
    27601 ccataccgcg ggcagcttcg catcgggcag cgcggccagg cagcccagcg tgaccagggc
    27661 gtgccgggac tgctggcgtt cctcgctgcg ataggcgttc agcgcggcgt cggccttggt
    27721 caggaagacg tcccggacct ccggccgcgc ggcgagggcg gtcgcggcgg cgccggtcgc
    27781 gaacagctgc ccggcgccga agcgcgagtc ggtcgcgctg cgggcgtcga catcggtctg
    27841 cagggtcgcc agcaccgagg ggaagcggcc tgcgttcacc gggatcaccg acagctcctt
    27901 gcgcttgtag ccgagggtga aggtggaggt gctgccgccg gcctcgtagc cgatcttcag
    27961 gcccaccgtg gtcgagctgc cgaagaacat cggcttgaag tcgcccccct gctccggatc
    28021 ggacggcagc ggcgccgcgg gcgcgagcaa cgcgcgggcg gcgtcgccgg tggcgtagag
    28081 ctgcttcacc tggcgatcga gcagctgccc gttggtcatg aacaccgcca acacgggcgg
    28141 caccgcgccg ctctcgtagc gcgggccgaa ataaccctcg acgcgatcgt aggcgatgct
    28201 ggccgatggc ggcgtctgct cgacgtccac gccgagggag ctcttggtca cgaagaccac
    28261 ggtgtccggc cgactcgcgc agccggcgac catcagcgcg agcgacagga ggatcgaaac
    28321 gcggcgagtg ctcatctgac gacctccgtt cgatctgcag cgggcgcgag gcaggcgcca
    28381 tcgcgcagga atccgagcgt ggtgtccgca tagcccagca cgagcgcgct gcccgcctcg
    28441 gcacgagtcc agcccaggcc cagggtctgc acgcgcaagg tctccccggc gaccgcgcct
    28501 tgcgccggcg ggagggtcag atgcatccag ccgagcaaat gccgctgccc ctcggcatcg
    28561 acccacacgt gcgcgcaccc gcctgccaag gccagcacgg ccagcgctcc acctcgcagg
    28621 atcgaatcga ccaggggatt catgtcaggc tcgatgaacc acgccggcgg aagccaccgt
    28681 gggcgcttcg cccggtgacg atccccggcc ctccgggagc accgccatgc gggtcacgct
    28741 tccagcatag cccgcggtcg tgggcttgca agccggccgc gccccgccgc atggcacgac
    28801 ggacgagcgc ggcggggagg ctcaggagcg cttcctccag acggtcagct ccgacagcgt
    28861 gtgctggtgc ttgcgccgtg tctcgcggat cacgaagggc accgcctgcg gcccctgcac
    28921 cagctcgaag tcggtggcga gcaggtcctt gagcccgtcg agggtcgtgt acgactcgcc
    28981 atccttcttg aagccgccga tccactcctc gcgcttggtg tgctcttcca gccaggtgta
    29041 gggcgaggcg agcagcagca ggccgccggg cttgagccgc cgcggcacgt cggcgaggaa
    29101 gcggcgcggg ctgtacaggc ggtcgatcag gttggcggcg aggatgaggt cgaagccggt
    29161 gaacacgtcc ttgaggttgc acgcgtcgcc ttgccagaac tcgacgcggc cggcggtccc
    29221 ggccagcccg agggcgtcga gccggcgctc gtggtaggtg accagctcgc cctcgtcggg
    29281 catcgtgtag cgcagcacgc cggtctcggc gagcttgacg ccagcctgga tgaagcgcgc
    29341 cgagaagtcg atgccgacca cgcgctcgaa ggcgcgcgcc agctcgaagc tggcgcgacc
    29401 ggtggcgcag ccgaggtcga gcgcgcggcc gaagcggccc ttgccgaagc ggcgctgggc
    29461 cgcgatcgcg atgtcggcga gcgccttcgg gaagttgggc acgccgaagg cctcgtcgcc
    29521 gtagtggaac tcggcgtact gcgacagcag ggtgtcggtc tcgtagctcg aggccgggtt
    29581 gataaccggc gcctcgctga cgacgtagcg caagcccgcg tgctggaaga aatggcggcg
    29641 gaaggcgtag cgcgaggcgt ggcgcgactc gttgccggcg gaaatccagc tgccgccctt
    29701 gatgatggca tggcggtcgt cgaaggtcgg cgtggtgaag tcgtcgtaga tcgggtgcac
    29761 gtcgaagccg tcgaagggat aggtcggggt ctcgcaccat tgccagacgt tgccgaccac
    29821 gtcgaagagc tcgccgtggg cgaagcgcgt caccggacag ctcgaggccc agtggtccag
    29881 atgcaggttg gcgttggcgg gggcgtcgtg cggcacatcg gcgaggcccg cgtggtcgtg
    29941 gatgcggttc cactcgtcct cggtgggcag gcgcaccggc tggccggtct cgcgcgcttt
    30001 ccagcggcag aaggcgcgcg cctccagaca gttgacctcc accggccagt cccagcgcat
    30061 cggcacttcc tcggtcatca ggcgcagctt ccagcccgcg gcgtcgggga cccagaaggt
    30121 cgggtgctcg gcgcgggcga acctgcgcca gcccaggccc tcctcgtccc agacgctgtc
    30181 gtcggcgtag ccgccggcct cgacgaagcc gaggtattcg cgattggtca ccagatggcg
    30241 ggcggcctgg aaggccggca cctcggcgcg gtgcgtgccg tactcgttgt cccagccgta
    30301 gatcggctcg tcgaagctgc ggccgagcgt gaccgtgccg gcggggatgt cgaccagcgc
    30361 gttggccggc ggctcgccat gctcggcgca gggctgccag gcgggatgcg gcttcacgta
    30421 ctgcagcgcg tgctggcgca tcagcaccga ggaggtctcg aggtggatgc gctcgtgctc
    30481 gatgcccatc accaccgccc agaacgggtc cttccagccg atcggcaagg tgaagggcat
    30541 gtcgcggatc acgcgctcga ccaccgcgcg caccttgccc cggtaggcgg ccacttcggc
    30601 cacgctcggc cagtcgtagc gcgcgtcgtc gaggtcgtcc cagctcatct cgtcgacgcc
    30661 gacggcgaac atggactcca ggcgcgggtc gatgcgctgc tcgatcaggc cggcgagcat
    30721 gaacttgttg atgaagaagg tggcggtgtg gccgaagtag aagatcagcg ggtggcgcag
    30781 gctgatcggt ttccggtagt aggcctcgtc gcaggtgagc acctcgaaca gcgactcgta
    30841 gcggtcgaag gtggcgtgga agtaatgcag cagctcggcg cgcttggcct cgacatcggt
    30901 gccggagagc agcggggtgc gcgggaagag gggcgcaggg ctgccggcgg aagaggaagc
    30961 gggggaagga cggtcgccag gggtcgcttg cgatgcgggg ttcattgcgg gggtcctcgt
    31021 cattgggacg gatgggctgc atctttccac gtttcggcgc gggaatgtag cgtgggccgg
    31081 catcctgcgc agcagcgaca cgagccgcga tctccgatcc tccagccggc gccgtgcctc
    31141 agcccgcgcc gcgatcgcga ctcgcgtcgc tcctaccgct cacaccggat gcggtcgcat
    31201 tttcgaatcg cgacttgcgt cgcgcctacc gctcgcaccg gatgcggtgg ttttgtggga
    31261 gcgacgcaag tcgcgatcag gccgcccaga actttcgcgc cctcgccgat cgagcgctgc
    31321 gatcgcgact cgcgtcgctc ctacgggggg ccgccgcgct ggcggaaggc gggctcgcgg
    31381 gcgaacagca gcgcggcgac cagcgcgttg agcaaggccg tggccaggaa gagctgcggc
    31441 accgcgaagc ccgcggccag cagcccgacg cccatcgccg cggcggcgac catgaagagc
    31501 gcgttgagga tgttgttgcc ggcgatcacg cgcgcgcggt gcgccggcgc ggagcgctgc
    31561 tgcacgagcg catacagcgg cacgatgaag aagccgccgc acaccccgat catcacgagg
    31621 tcgaacacca cccgccacac ctcggcgcgg gcaaacagcg tatccagccc ctggccgggc
    31681 gggaaggcgc ccgccgccgg gctcgcccac cacaggtcga gcgcgaacag gctcagcccg
    31741 atcgcccccg gcagcaccat gcgcggctcc acccgcccgc gcgacaggcg cccgcacagc
    31801 agcgagcccg cgccgatgcc gaccgagaac agcgccagca gcagcgtcac cgcgcgctcg
    31861 tcgccgccga gatgatccgc ggcgaagccg ggaaactgcg acaggaacac ggcaccgtag
    31921 aaccagaacc acgagatgcc gacgatggcg acgaagaccg cgcgcgcctc gagcatgaag
    31981 cgcagcatct cgcgcgtctg cgtcaccggg ttccagttca cccgcagccc ggggtcggcc
    32041 gccgccgcgg gcgggatggc gcggctggac caccagccga gcagcgcgat cgcgagcacc
    32101 gcgaccgcca cacggccggg cccggcatcc cccgccacca gcaggccgcc gacgatggtg
    32161 ccggcgagga tggcgacgaa ggtcgccgcc tccaccagcg cgttgccgcc caccagctcg
    32221 tcgtcggcga gctgctgcgg gatgatcgcg tacttgaccg gcccgaacag tgccgactgc
    32281 gcccccatca ggaacagcac gacgagcagc agcggcagcg acatcagggc gaagccgacc
    32341 accgcaagcg ccatcacggc gatctcgagc agcttggtgg cgcggatcag gcggctcttc
    32401 tcgtacttgt cggcgagctg gccggcggtg gccgagaaca ggaagaaggg caggatgaag
    32461 acacccgcgg cgaggttcac cagcaccccg ggcgagtccg cccccagacg cgcggcctgg
    32521 aaggtgatca gcaccaccag cgcgttcttg tagacgttgt cgttgaaggc accgaggaac
    32581 tgggtcagga agaagggcag gaagcggcgc tcccgcagca ggcggaactg gccgctcatc
    32641 gtgcggcctc gcggaaatgg gcaagcgccg tgcggccgct cgcggagatg ggattggcgg
    32701 gagtcttcat cgcccggcaa ggtaatcgga aatgggcccg gtcgccaccg ccgccgcgtc
    32761 acctgccccg gagcgacgcg ggatgcatcc ttgccgaagg agcggcgcat tcgcgatctt
    32821 cggcgccgaa tccgccgagc cggggcggcg cggaccgggc ggggggggga caatgcccca
    32881 cctcctggcc tacaatgtcg cctctttcca ccgatcgagc gccgcatgac cctgcccgcc
    32941 cgccttcgtt ccgcccatcg tcgcgccgcg ttgcgcgcgg gcgtgcgtgt gcgtgcgcgc
    33001 ggcatcgtgt cgccgctgcg cgatctcgcg acccagctcg cattcaaggt cacagggtct
    33061 cccgccgtgc tttcccaggc cgtgcggggc caccaaaggg gctgagcccc acccccgcac
    33121 ggcaccttgc ccgggacgcg cacgccgcgc ctcccgttca acgtgcagcc gccgcgggtc
    33181 tttctccagc ttcccgccca tcgaccgctc atcgaccaga ctggcggtcc ggccgcggtc
    33241 catccggacc ctcccggcca tcggcactgc cccacggggc tgcacccgga acgccacgcc
    33301 cgccgcgtgg cgatgcgatg cacgagggtg atgcgatggt tctcacaaga agtttcgtca
    33361 gggcgaagcg gccctgtacc gatggattcc gctggttcgt ccgccagttc ggcgaaggcg
    33421 gcgactacca ggccatgctc gacgagatgg tcgccgccgg ccgcgtcagc gacgcctgct
    33481 ggctgctcga gcagttcggc cccaccgacg agttgcgcgt ggtcgacgcg atcgaggccg
    33541 acgcgatcgt gttcgccggc accctcgagg tgcgcggcgg cgtggacgtc gacggtgtgc
    33601 tgcgtgtcgg ccgctcgctg caggccggtg ccggggtgcg cgcgggtggc gaagtccaca
    33661 tcggcgccga cctgcgtgtg gaaggcagcg tgcgcgccga ggccggcctc cagatcggcg
    33721 gcgacctgcg cgtgggctgg ggcgtggact gcgcgggcga gctgcgctgc gacggcgatc
    33781 tgcgcaccgg ctgggacctg cgctgcggcg ggcgcatgcg cgtcgccggc aacgcgtacg
    33841 tcggcctcga cctgcaggcc gaagagggcg tgcgctgcgc caagggcctg caggcgggcg
    33901 gcgacatcca ggtcgagaac accttgcgcg ccgcacaggg catcgccgcg ggcggctcga
    33961 tccgttccgg catgcacctc gaggccggct ggggcatcaa ggccggcggc gtgatcgccg
    34021 ctgcgggcgc gatccgtgcc ggcgagagcc tggcggcggg cgaggcgatc cgtgccggca
    34081 ccggctatgg cgtattcgcc ggtctcgacg tgcagatgga agcctgggag gcgagcgcgc
    34141 gagtgagcgc gccgcagcgc cccgagggcc tgatgagcgg ctggtgggcc ggcccctcgc
    34201 cgtgctgaac ggaggacgcg tcatgaacac tctcgccctg cacctgcgcg gcctgcccgc
    34261 ccgccacacc ccgctctccc accgcgccag cctctcaccg cgctcggtgc tggccttccc
    34321 gcagccgccg ccgttcgttg ccgggccggc cgtggccttg cgcaccgcac tcggcactgc
    34381 gctcgctacc gtcctgcgtc gcatgctgcg gccgccgcgc atggaattgc gcatcgacgt
    34441 gagcccgccc gacactgcca tcgaggccga actcgacacc ctccacggcc ggctccacac
    34501 ccccggcgac gccctgcacg cctgcacccg cctgcccacg ctgctgcccg acctggtctt
    34561 ccaccaccgc gaggccgacg gcgagcacta cgtctatgtg gaagacgtcg cgcgtgggtg
    34621 cctcgccggc tacacggtgt tcaaccgcct gatcgaggtc gaccgccgca ccgaccgcca
    34681 cgtgcgctca ccgcactcca agtacgcccc cgcctaccag ggccgcggca tcgccagcgc
    34741 ggtgtatgac tgggcgctcg ggcgcggctt ctgtctggtc agcggcgccc gccagtccgc
    34801 cggcgcgcac gcgctgtggc acgccctcgc ccgccgccac cccctgcgct gggtcgcgct
    34861 gcgcaccaag cgcatgcacg acctcggcgc cagcgtcgcc ccgacgcgtg cagccgaact
    34921 cgacacccgc atgatcctgc tcggccacgg ctggagcgcc gcgcgcctgc gcagcctcgg
    34981 cctgctccat ccggccgccg aggccgccaa cgagacgatg cgccggcgcg cgtgaaacc
//
LOCUS       DAOQBS010000018        33676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_710_length_33676_cov_16.585884, whole genome shotgun sequence.
ACCESSION   DAOQBS010000018 DAOQBS010000000
VERSION     DAOQBS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 33676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33676
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_710_length_33676_cov_16.585884"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1146)
                     /locus_tag="PKN25_03835"
     CDS             complement(<1..>1146)
                     /locus_tag="PKN25_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HNV90058.1"
                     /translation="DAKDGGAPQLVAVKQGETVSTEDIVVKSLQTKPPARFNEATLLS
                     AMEGAGKMVDDEELRAAMAERGLGTPATRAQIIEGLISEQYIHRDGRELIPSAKAFSL
                     ITLLKGLGVTALTSPELTGGWEYKLAQMEHGKLSREAFMNEIAEMTREVVERAKRYES
                     DTVPGEFVTLQTPCPKCGGVVKENYKKFACQACDWSTWKIVAGRQFEYDEIETLLRAG
                     KVGPLLGFRNKMGRLFNADIVLNEDKQPTFDFGQPKEGEEAEAVDFSDQQPVGACPKC
                     ASRVFEHGMAYVCEKSVGPGKSCDFRSGKVILQQPIEREQMARLLTEGKTELLKGFVS
                     ARTRRKFSAFLVRGKDGKVGFEFEAKAPKAGAKPAAGKGDEAAGEDA"
     assembly_gap    1147..1246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1247..2625)
                     /locus_tag="PKN25_03840"
     CDS             complement(<1247..2625)
                     /locus_tag="PKN25_03840"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNV90059.1"
                     /translation="MSKQLIIAEKPSVAQDIARALGGFTKEKDYFESDEYVLSSAVGH
                     LLELTLPEEFEVKRGKWTFAHLPVIPPHFAVKPIEKTEDRLKLLTRLIKRKDVTGLIN
                     ACDAGREGELIFNFIAQHAGTKKPMQRLWLQSMTAQAIRDGFAHLRAAQDVEGLRNAA
                     ICRAESDWLIGINGTRAMTAFNSKTGGFHLTTVGRVQTPTLAMVVEREDRIRKFKSRD
                     YWELEARFGCAAGEYPGRWFDEKFKRPEGDEHATAFRLWDKAQAEAIRAKCEGKPGVV
                     SEEAKPSTQLSPLLFDLTSLQREANGRFGFSARVTLQLAQALYEKHKVLTYPRTDARA
                     LPEDYLATVGEVMRTLPDQYAPFANEITRQGWVRPNKRIFNNAKISDHFAIIPTGALP
                     KSLSDAEHKIYDLVTKRFLAVFYPAAEYQITTRITRVEGEAFKTEGKVLVNPGWLTVY
                     GKEAANDEK"
     gene            complement(2729..3199)
                     /locus_tag="PKN25_03845"
     CDS             complement(2729..3199)
                     /locus_tag="PKN25_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HNV90060.1"
                     /translation="MFDILVYLFESYIHADACPEGELLNRKLSAAGFEQDDISEALEW
                     LAGLRRVARDIHPGTAPQAGSVRIYTEEEQALLDGRCRGFLSLLESAGVLGTAHRELI
                     IERAMALADFNITLNRLKVIVLMVLWQQEEPIDTLMVDELLTEEDDWAYEPTVH"
     gene            complement(3283..4446)
                     /gene="dprA"
                     /locus_tag="PKN25_03850"
     CDS             complement(3283..4446)
                     /gene="dprA"
                     /locus_tag="PKN25_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNV90061.1"
                     /translation="MTAPADDLADWLRLACIPGVGTQGQRALLAAFGLPGHIFAAGRG
                     ALAAVVGGQAADAVLAVPAHEDIERTLAWASEAGNRVLTLADADYPRQLFDIADPPVL
                     LYVKGEPALLSRPGIALVGARSATAAGEANAEAFARTLAQQGLVVVSGLALGIDAAAH
                     RGALAAGSTGAGTVAVIGTGIDRIYPARNAALAREIAAAGAVVSEFPLGTPPLQHNFP
                     RRNRLIAGLAEGVLVVEAALGSGSLITARLATETGREVFAIPGSIHSPLSRGCHRLIR
                     DGAKLVETAEDVVEELRGRLGWPAPVAASVKDRRSRGAAPAVPAAPPRQAALALDGER
                     TRVLEAIGHDPVDLDTIAARCGLTVDALYAILLPLELEGRLAKLPGGRFQRIT"
     gene            complement(4478..5518)
                     /locus_tag="PKN25_03855"
     CDS             complement(4478..5518)
                     /locus_tag="PKN25_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNV90062.1"
                     /translation="MMRIIFPLLVAFASLLGPAAHAQSNVRLAPDAPDSYTVVRGDTL
                     WGISGRFLQEPWRWPEVWRLNRDEIRNPHLIYPGQVVLLDRSGPWLSVGRRVGDGGTR
                     DGRLQPKVHSEPIAEALPSIPLQIIEPFLNRPLVVDQPRLDGSATIVATETSRVLTGT
                     GDTVFAKNIGDDADIWHIYRPARALEDPQTREAIAWEAAYLGTARVTERGEPTTLEIV
                     SAVEEIGTGDLMMPSEPPSVFSYAPHAPDFEVEGRILSIYRGVSETGRLNVVALNVGE
                     QDGLERGHVLALHRNRGVAEYRGEDGKESFRLPEKRYGVAFVFRVFERVSYALVMESD
                     GPVTIGDTLRKP"
     gene            5711..6214
                     /gene="def"
                     /locus_tag="PKN25_03860"
     CDS             5711..6214
                     /gene="def"
                     /locus_tag="PKN25_03860"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNV90063.1"
                     /translation="MALLPILRYPDPRLHTVAAPVGRVDDEIRRLIADMAETMYEAPG
                     IGLAATQVDVHKRIVVIDVSEDKSGLMALINPEILERSGEQVCEEGCLSVPGIYEKVS
                     RAERVKVRALNEKGESFEFEADGLLAVCVQHEIDHLDGKVFVEYLSQLKLGRIKSKLA
                     KKARITA"
     gene            6214..7176
                     /gene="fmt"
                     /locus_tag="PKN25_03865"
     CDS             6214..7176
                     /gene="fmt"
                     /locus_tag="PKN25_03865"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNV90064.1"
                     /translation="MAATASGAGGLRVAFAGTPEFAAQALDAILKAAYAVPLVLTQPD
                     RPAGRGMKLSPSAVKQLALAHGIEVDQPEKLRTEEQRARLVACAPDVLVVAAYGLILP
                     PAVLALPRLGCINIHASLLPRWRGAAPIHRAIEAGDAETGITIMQMDEGLDTGPMLLR
                     RALPIAADDTTASLHDRLAVLGGACIVEALAALAAGALVATPQPAEGVTYAAKIGRAE
                     ADIDWSRPAADIERAMRAFDPFPGAVSTLRDTAVKCWKARVVAGEGEPGRVLAVDADG
                     IVVACGRDALRCTVLQRPGSKRLPAGEFLRGFPVSVGERFASAA"
     gene            7267..8106
                     /gene="htpX"
                     /locus_tag="PKN25_03870"
     CDS             7267..8106
                     /gene="htpX"
                     /locus_tag="PKN25_03870"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNV90065.1"
                     /translation="MFGNWIKTSILMAGIVALFGAMGAAIGGQQGMLIALVMGGAMNV
                     WAYWFSDKMVLKMYKAREVDAASSPYLYNMVAELARRAELPMPKVYIIDEAQPNAFAT
                     GRNPDNAAVAATTGIIRMLSERELRGVMAHELAHVKNRDILISTISATIAGAISMLAN
                     FGMFFGGREGEERPNPIVSIAMMILAPLAGMLIQMAISRTREFGADRGGAEISGDPAA
                     LAAALAKIDAYARGIPMHTAERHPETAQMMIMNPLSGGGLRGLFSTHPSTQERIARLQ
                     TMR"
     gene            complement(8121..9272)
                     /locus_tag="PKN25_03875"
     CDS             complement(8121..9272)
                     /locus_tag="PKN25_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNV90066.1"
                     /translation="MEPVDLFYSYAHEDEKLRDELDGHLALLRRKGVIRPWHDRGIVP
                     GQEWDKAIDEQLAMADLILLLVSMDFLNSDYIWGKELATAIERARRGDASVIPVLLRA
                     VDLEDAPFAHLQGLPTDLRPVTSWPNRDEAWTDVAKGIRRAVEAIRQRWASAPPPAAP
                     PAAPPEAPSVPRASGFGTAVASVPLPEEPVRTLEMARPAPRMAATRDARTDALLERTI
                     AAFANELALAARIKGVQGIGSGEAEGLAEALIDMPAQKRVLWVDDRPENNRHELAALA
                     KLQVEVVNVRSTGEAIACLDADEEGFDLILSDWDRAEPAATASSAGLELLRMLAARPR
                     RPPVIFYHAEFDPSRRAARRECLLRAGAFGEAVRPGELLALIRSAFASD"
     gene            9515..10015
                     /gene="tpx"
                     /locus_tag="PKN25_03880"
     CDS             9515..10015
                     /gene="tpx"
                     /locus_tag="PKN25_03880"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNV90067.1"
                     /translation="MSKVTLGGNPIDVAGRFPQAGEAAPAFRLTGADLADVGLEAFAG
                     KRKVLNIVPSLDTPVCATSTRKFNAEAAGLADTVVLVVSADLPFAAKRFCETEGLKNV
                     HTLSTFRNPKFAQDYGVAITSGPLAGVTARAVVVVDGNDKVVYSQLVPEIKEEPDYAA
                     ALAALK"
     gene            complement(10059..11756)
                     /locus_tag="PKN25_03885"
     CDS             complement(10059..11756)
                     /locus_tag="PKN25_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNV90068.1"
                     /translation="MDKQPTEGPELEVSFDELTRVLSVSIAHDPHFPRIDALWLRRRL
                     EAAGYADLQIRPDPIRRLIAQYNAGEAVAPVEIAQCVDASMQIGVSLDGLVARLSIVP
                     AKGGKPASKTELLALIESRGIVEGLLLEEMNRAIADGQADDLAIARGREPEPGKDGWL
                     EYLLPETRERVPSVRPCGRTDYRDLGEILVVHAGDALMRRHPPQPGVDGVNVYGRPIV
                     ARRGREQRFAPGLRGTAISPEDPELLVAACDGQPVRVRNGAMVEPIFTVDAVNLATGN
                     IDFDGSVRIRNDVQAGMTVRASGDIEVGGVVEPATLEAGGSIVVKGGVLGGLGGKTAG
                     KDYSAHAIRCEGSFCATYAQQARISAGDSIFIDDVAMQCQLEARNHIRVGKRLRGQIV
                     GGHCRASLSIHARTIGANSRIRTELEIGMDNGLEHAIQEKAEARDALENRLLEIGKML
                     TFADRHPDRVTPEMLGRAEQTASALSGEIESLRSEEEDLQHRLALTREARVNAEREMF
                     EGCIVRMGEQLFKLSQDRGPTTVRLATQGLGVFPLEDDSRFDEPQRPAAASQGSSRR"
     gene            complement(11883..13814)
                     /locus_tag="PKN25_03890"
     CDS             complement(11883..13814)
                     /locus_tag="PKN25_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNV90069.1"
                     /translation="MSAAPPSAHPIRVLSVIPPMTQLNTPYPSTAYLTGFLRSRGVDA
                     VQEDLALKLVLRLLSPAGLDDIRARAEALPKKRRTPLVQGFLDHFARYRYTVDPTIAF
                     LQGRDPTIAHRIASRTFLPEGPRFDAVDAYVDPDDPEGGDPLAWAFGALGLADRARHL
                     ATLYLNELADILRDAVDARFEFVRYAETLAMSQPTFEPLAAALAAAPTLVDAYLKDLT
                     LEALSRHSPQVVLVSTPFPGSVYGAFRIAQAIKAAHPEIVTVLGGGFVNTELRELAEP
                     RVFDHFDYVTLDDGERPLLALLEHLQGRRGRSRLVRTFVRDADTGQVRYINLAEPDVP
                     FAEVGTPTWDGLPLDGYLSVLDMLNPMHRLWSDGRWNKLTVAHGCYWKKCSFCDVSLD
                     YIGRYDGAAAGLLVDRIEAIIAETGQTGFHFVDEAAPPKALRALAEELLRRGLAISWW
                     GNIRFEKSFSPELCQLLADSGCIAVSGGLEVASDRLLQLMKKGVSVEQVARVTHAFTA
                     AGVLVHAYLMYGFPTQTVHDTVDALEYVRQLFEAGCIQSGFFHRFACTVHSPVGRNPE
                     EFGVTLVALPEITFAKNDVGFIDPTGVDHDRLGKALNKALYNYMHGIGLDTDVRAWFE
                     DKVPRPRVARHFIARALGA"
     gene            complement(13856..15355)
                     /locus_tag="PKN25_03895"
     CDS             complement(13856..15355)
                     /locus_tag="PKN25_03895"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNV90070.1"
                     /translation="MNGHAPHFDVEDFADPAAALARAHEIYDLAVEHLRRGLQHYVDG
                     ADIGRHVRACYPLLRVRTDTVARADSRLSYGFVAGPGVFETTLTRPDLFADYYLEQFR
                     LLVQNHGVALQVGSSTQPIPVHFALPEHDYLEGHLGPERRRLLRDHFDLPDLGAMDDG
                     IANGTFEPGPGEPHPLALFTAPRVDYSLHRLRHYTGTRPAFFQNFVLFTNYQFYIDEF
                     IRLGHELMADTASGHGYEAFVEPGNVVTRRSDLAPQAEDADGTPPPRLPQMPAYHLVR
                     GDHAGITMVNIGVGPANAKTITDHIAVLRPHAWIMLGHCAGLRNTQQLGDYVLAHGYV
                     REDHVLDEELPPWVPIPPLAEVQVALEAAVAEVTQLSGYELKRLMRTGTVASTDNRNW
                     ELLPSHGMSSSPERRFSQSRAVALDMESATIAANGFRFRVPYGTLLCVSDKPLHGEIK
                     LPGMADKFYRERVDQHLRIGIRALEQLREQGVDRLHSRKLRSFAEVAFQ"
     gene            complement(15376..16167)
                     /locus_tag="PKN25_03900"
     CDS             complement(15376..16167)
                     /locus_tag="PKN25_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNV90071.1"
                     /translation="MTLPTVHDARLQLDKRVATLTLARDDVRNALTGTALIDDIVGVA
                     EWVNRCEEVSVLVITGEGAAFSAGGNVKDMAARGGDFAGDVAEVATRYRQGIQRIPLA
                     LQAVEVPVIAAVNGPAIGAGFDLANMADIRIASTKAKFGETFLNLGIIPGDGGAWFMQ
                     RLIGYQQAFELTLSGRIVGAAEAKTLGIVLEVVEPEQLLPRAHELAARFAAQPPKALR
                     LTKRLMKMAQRMELKDFLDLSAAFQGMCHNEPEHLEAVQRLLART"
     gene            complement(16254..16754)
                     /locus_tag="PKN25_03905"
     CDS             complement(16254..16754)
                     /locus_tag="PKN25_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNV90072.1"
                     /translation="MSLAVRPSASSFTRSLLAVLASVLLAGCASLIQREPVRINVVGL
                     EPLPGAGMEMRFAVKLRVQNPNEAAIDFDGLALELDLDGKPFATGVSDRKGSVPRFGE
                     ALVEIPVSVSAIAVVRQALGVIEGEPKTEVPYTLRGRLAGGLLGGTRFSDAGMLKLPE
                     PLRNPR"
     gene            17001..19601
                     /locus_tag="PKN25_03910"
     CDS             17001..19601
                     /locus_tag="PKN25_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNV90073.1"
                     /translation="MVSAPRAHPGVAPACFALVPAAATAGAVLARYGELALVEVERVP
                     VTAWASWTDLDPIRVHGVPRILPSGCFEPGAGPRCILRFAGPVLPAWRETLATLGWTP
                     RFDCPPSGLCADVGGDLVALRAALPALRAIAPYAAEDCARAGAGGVAALPDTVDAVAF
                     SVEDRVRLEGELTAAGLQVVASSPYKLRVSGDAGAVGRLRDAVGVKIADPGTLPRTAS
                     LSLRLAIGATALPESAGGATGAQGGEGELIAFADTGLGTGATDAPHPDFAGRVQAIHS
                     WPLAPGWSAFVRAPGADDGGADRGSGHGTHVAGLAAGDGGLSAGRLVGVAPGARILMQ
                     AIEQYVDPLPEWRSRLQPGFYLAGRPLDLRELLEQARAAGAAIHVDAWGVEAAGAYTD
                     DSWECDHFLHAHAEMLVIVAAGNAGRDRNRDGAVDAGTLYAPASAKSVLAVGATEGPD
                     AGIGLRGGWAALDPAGTRFPDPAQRGASVSGDAERLALLSSCGPTLDGRIKPDLCAPG
                     TNLAAARSTASSARGWGLANPLPWYQYLGGTSMSTGVVAGAAAVLRQRWRPVLGEAPG
                     GAALKALLIHGARAVHRRDGQGEEDRQRAGFGRLWLAGSLPGPDGPVLIEDRVGVATG
                     ERRRIDCTGPGRLRAVLCWYDAPGEALINDLDLVLCDAGGSVLAWGNHPAGAVGSPDR
                     RNTVECIDVALGEGEWMLEVRGVNVPLGPQPWALATSPGVVVRATPAAPGTPTVPADP
                     LPAPRWRVDLPWTAVRGVGARRAIELERTLPGGLAALCAGAPDEDALAFLPAALRAQI
                     RSVAAFLGAPPPAAARLALAHPLHALLSGAPETVSTVAWQALAAALSPLLEALDRNVA
                     REVRGERLFR"
     gene            19623..20642
                     /locus_tag="PKN25_03915"
     CDS             19623..20642
                     /locus_tag="PKN25_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90074.1"
                     /translation="MADNTEKPGKGKPTRTQAGATSPAAALPEGSILSAGALMPGAVG
                     GALALGADVSGELAELAPSFGDILASIGRGVADSQTALDRGLVDTATTLSQSNITVVT
                     DVVQELNDDGLPVPSATQLVTQNVSLINFVRPTAHQWSHVALRMDMTVGAVDNERGIS
                     VNSKTKSARATNVGLFFGFLGIGVSSFKEKSTFRSEESDFESNWAEGRVLMDALLQPR
                     NVDGFDPPAEVSIGPQIFMALGSVQETLNSGVVTARSTDIVITVRKANGAVNPTVTID
                     VDPGPFDLSFATADGFTGNTTNAAGQCRITLSRNIPSPLFQRRVRGTVTATLGQIERS
                     TTVSL"
     gene            20670..21563
                     /locus_tag="PKN25_03920"
     CDS             20670..21563
                     /locus_tag="PKN25_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90075.1"
                     /translation="MPDLLPPLARALAEDANVSDELRGAGIAFGTLVERTGAAVAATQ
                     LQLDTSSAATASALAATLVNVIAVREQVFRDDGTLDHVTNHVRPLPLISFIDPVFYQW
                     TSVRLQGEFYAREFAASSETTSAYSGSNFGLFGSGVSMFFGPGMVTSGSGSGSSTTTT
                     DVSTDVSLGRIRASALLEPRDDVGIPKPNQVIRGPRLALIQGEIADVMDGGDLVGRTM
                     SVLLQYSRRNGAPIAGKALSVESSGVPWRHLGSGVTDAAGQLQIELRRDFLDEDADTA
                     PASFVITARIGLVSNSVTVTF"
     gene            21616..22008
                     /locus_tag="PKN25_03925"
     CDS             21616..22008
                     /locus_tag="PKN25_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90076.1"
                     /translation="MDKANVSAGELAGQQAVELGELLARTVVSVAAAQEALDRHATDR
                     LDAIERGDEDAPRVPPLWYTFTEVTLEVALSASVERASGSVPPRLMSRPVDARQVSLY
                     GYQAASSLMVRLRLAPRQGMPDVSPPPA"
     gene            22047..23009
                     /locus_tag="PKN25_03930"
     CDS             22047..23009
                     /locus_tag="PKN25_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNV90077.1"
                     /translation="MPIDPKDLRTAAGSLLDPRLVDTMNLKPVGTSSLVNLEGRLGVF
                     TPVAEDGSGADNTEAVIAALRKELVRLESENAALRRQLAEVEAARKRSPDDFASAVAH
                     TLDTLQHKLAQTTNPVSRFAVREVAIDAQVTVDVSPLGTLEYRFVRPEDDIDPSKLSR
                     IRLDLVPLPREDAGLSASPEFSPWAGVEDIVGVGETWRGALNRQQIFTIADLLVAGSR
                     LKSSAELAALVGVDRQRLAEWMGHAELLSVRTIEPRAADVLWTVGIRSLETLAGAAAV
                     EVVEKYNAEVARRAHKSLAPIDAAQAASWIEAAKKYHGRPREGG"
     gene            complement(23017..23721)
                     /locus_tag="PKN25_03935"
     CDS             complement(23017..23721)
                     /locus_tag="PKN25_03935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HNV90078.1"
                     /translation="MKAHILQHVPFEGPGHIGDWLAARGADIGSTRFWESPVLPDPAA
                     VDLLVVMGGPMSVNDEAELPWLVAEKRFIAEVVALGRPVLGICLGAQLIASAHGARVY
                     RNADKEIGWFEVAASGAEPDAADTASRFPFPARTTVLHWHGETFDLPAGARLLASSPA
                     CRHQAFQLGGRTIGLQFHLEMTASSLRAIVDNCRGELVPGRWIQDEATILGHADGHFA
                     ATHALMDGLLDWLVRA"
     gene            complement(23760..24368)
                     /locus_tag="PKN25_03940"
     CDS             complement(23760..24368)
                     /locus_tag="PKN25_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HNV90079.1"
                     /translation="MDTPATIHAFWFGPAADADAEAAIIERQSALWWRKQPAVDAEIR
                     ARFAPLVGRAAGGELDTWLGGLRGRLALVLLTDQFPRNIWRGEAAAFAFDVLALRWAK
                     EALARGLDAGLRPIERVFLYLPLEHSENLADQREAVRRFDTLATAVEPALRTAFAGYL
                     DYARRHLEIIERFGRFPHRNAALGRETSPEEAEFLRQPGSRF"
     gene            24576..26507
                     /locus_tag="PKN25_03945"
     CDS             24576..26507
                     /locus_tag="PKN25_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNV90080.1"
                     /translation="MPSDTASPAPLSALRGLLPFLRPYRARVVLAFVFLCLGSAAILV
                     VPLAFRDLIDAGFGGVSVGGAATGGAADGGGLFGAQGLDGRFVALFGLAVFWAAAVAA
                     RYYTVSWIGERVTADLRSAVYARVLRQSPQYFETLQTGEVLSRLTGDTTLVQTVVGSS
                     ISLGLRSLFQFVGGMVMLAVTSVQLFGLIFGLMALLALPITLIGRRVRSLSREAQDRI
                     ADASALAGEILAAMPTVQAFGQEDNEAARFAARAETSFAAAVRRTRVRAALTGLIIAA
                     VMGTIIFVLWIGARQVQAGALSVGDLGAFVIYAALVAGGAGTLAEVWGDVMRAAGASG
                     RLLELLQAEPVIRDAEAAEGVAEAAGGGGSRRAPRLHENPRSTRPAGAAPAANPAPDS
                     PTKAPATHPAIELADVVFRYPARPAVAALDGIDLRIAAGEKVALVGPSGAGKTSLFQL
                     LLRFYEAGEGCIRIDGHDIRALPLRSLRARIALVPQDPVIFSASALDNIRYGRPDAGD
                     AEVIAAARAAAADEFIARLPEGYATFLGERGTRLSGGQRQRIAIARAILKGAPILLLD
                     EATSALDAEAEVLVQQGLAAAMQGRTTLIIAHRLATVQKVDRIVVMDAGRIVETGTPA
                     DLRRQGGLYARLAALQLEG"
     assembly_gap    26566..26665
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(26736..27632)
                     /locus_tag="PKN25_03950"
     CDS             complement(26736..27632)
                     /locus_tag="PKN25_03950"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HNV90081.1"
                     /translation="MRPPTRIPAPALRRPGSCIRSLILALALLPAGAVAQTAEPADAP
                     AAMLALRYDPAIDPAGWWVSEKLDGVRASWDGSVLRFRSGRALPAPAWFLAALPPVAL
                     DGELWLGRRRFDELSGLLRREDPADPLWREVRYMLFELPGAAGDFDTRLARLRSLAEA
                     ADAPWVQAIPQQRIPDRAALQRLLGEVLAGGGEGLMLHRADALWQPGRSEALRKLTPW
                     LDAEARVVGYLPGKGRLQGRVGALQVERADGRRFRIGSGLSDALRANPPALGALVTYR
                     YRELTPAGLPRFPRYLRERTLP"
     gene            complement(27831..28664)
                     /locus_tag="PKN25_03955"
     CDS             complement(27831..28664)
                     /locus_tag="PKN25_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HNV90082.1"
                     /translation="MNDALAIPAAFAERRLFEPIAPWLARFTTPGLPSRAALDALLAE
                     AAPAARSGGGAPIRFAAPGDDEPGYEARIFATGEVPTREADWHDFFNALAWCAWPRTK
                     ACCNRLHLDELRARDAAGLAGRGPRRDALTQFDECGIVVVSADPQVPALLAAHAWEEV
                     FWNRREALQHSTRFLVFGHGTWDQLRAPFPGLCAKAIHRVVDPAWLALPAAAARAETD
                     AWLAARLAATIGDFSPRSLAPLPLLGIPGVTPASESAGYYRDTRQFRPQRMPAGTAAG
                     A"
     gene            complement(28872..30212)
                     /locus_tag="PKN25_03960"
     CDS             complement(28872..30212)
                     /locus_tag="PKN25_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004387139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNV90083.1"
                     /translation="MKNIKLALLLVLATLSLLWWWADPLLVQGDTYFALRTLAVNYTG
                     VLAMGAMSIAMILALRPAALEPLLGGMDKAYRLHKWLGIAGLSVGVVHWLWAKGTKWA
                     VGWGWLQKPARGPRPEQTVELFRFFQEQRGLAETIGEWAFYLFAVLAAIALIKRFPYR
                     RFVQTHRVMAAVYLALVVHGVVLLPFAYWTQPLGMVMGMLMAAGSWAALRSLAGRIGR
                     GRRAHGRIERVVRHPANQVLEVGLRLDTPWPGHQAGQFAFVTFDEREGPHPFTISSAW
                     GGDGRMDFMIKPLGDYTRSLPATLKLGDPVRVEGPYGRFDFGGAQARQIWVAGGIGIT
                     PFVARMQARAQEDRPGGRAAAIDLFYSTSAPDEGFIARLRELAARARVQLHVLVSPRD
                     GRLDADGICRSVPDWKQAGVWFCGPAGFGAMLRDTLAARGLPAERFHQELFEMR"
     gene            30415..31191
                     /locus_tag="PKN25_03965"
     CDS             30415..31191
                     /locus_tag="PKN25_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNV90084.1"
                     /translation="MFQHSAPSPAWLQHDMERVDAIIRAHMNNDVALIRQVVEFILHG
                     AERRRPALVLASARALGHGGERPHALAAAVELIHISLALHDDVTEASGGAADGEREGA
                     LFGNAGNILLGDLLYTSAFRMIVDLGDMEVMRVLSDATNVIAEGEVAYRELLAAPASR
                     TPEAWLDIARRRRAKLFEAGAECMAIVHGADPAARSALARFGLHVGSAHALRSEAEDA
                     ARFGIADAQARAQAEIEAARAVADGLALANAAALGVVVEG"
     gene            complement(31573..31977)
                     /locus_tag="PKN25_03970"
     CDS             complement(31573..31977)
                     /locus_tag="PKN25_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90085.1"
                     /translation="MRSMKKPVRAASGALLFVAALATGACGLLPQKTEVAPGVTQQTG
                     QFEFALPSGEYRCERGERLQIRRELANAVNNRIQLGWNGSQYQLERDLSYSGLPRFED
                     GASGLVWIDLPWKGLLLDGRTHKPLANECRAA"
     gene            complement(32048..>33676)
                     /gene="gpmI"
                     /locus_tag="PKN25_03975"
     CDS             complement(32048..>33676)
                     /gene="gpmI"
                     /locus_tag="PKN25_03975"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNV90086.1"
                     /translation="AGVQGPVVVIVMDGYGIPKHEVGSAIDAARKPTLDRLFAQYPNI
                     VLRAHGTAVGMPSDDDMGNSEVGHNAIGAGQVYAQGAALVANAIAEGAIWQGEAWQQI
                     VAGAKTGANGKAGVLHFIGLFSDGNVHSHIDHLKAMVLRAKEEGVPTVRIHALLDGRD
                     VPETSALDYVVPFEAFLKDISTAGFDARIASGGGRQTITMDRYDANWPMVARGWRTHV
                     LGEGPQFASATDAVHGLREKHPGTIDQDLPEFIVAEDGRPIGTIEDGDAVVFYNFRGD
                     RAIEITRAFVEEHFTEFDRVRHPRTTYAGMLQYDGDLQLPKRFLVAPPAIKDTSSEWF
                     SKSGLAQFACSETQKFGHVTYFWNGNRSNKFDGETWQEVPSDVVPFEQRPWMKAAEIT
                     DAMIAALQSGRFKVLRCNYANGDMVGHTGNFRAATMAIEAVDLALARLLPAIDAAGGV
                     ALITADHGNADEMFELDKKTRQPAQNKDGSYKAKTAHTLNPVPLILYDNVSGGKLGLK
                     PLETAGLSNIAATVANLLGLHKHDKWDDSLLEVK"
BASE COUNT         4604 a        11487 c        12348 g         5037 t
ORIGIN      
        1 tcggcgtcct cgcctgcggc ttcgtcgcct ttgccggcgg cgggcttggc gccggccttg
       61 ggtgccttgg cctcgaactc gaagccgacc ttgccgtcct tgccgcgcac caggaaggcg
      121 gagaacttgc gccgggtgcg cgccgatacg aagcccttga gcaactcggt cttgccctcg
      181 gtcagcagcc tggccatctg ctcgcgctcg atcggctgct gcaggatgac cttgccggag
      241 cggaagtcgc agctcttgcc cggtccgacc gacttctcgc acacataggc catgccgtgc
      301 tcgaagacgc ggctggcgca tttcgggcag gcgccgaccg gctgctgatc ggagaaatcc
      361 accgcctccg cttcctcgcc ttccttgggc tggccgaagt cgaaggtggg ctgcttgtcc
      421 tcgttcaaca cgatgtcggc gttgaacagc cgccccatct tgttgcggaa gccgagcagc
      481 gggccgacct tgcccgcgcg cagcagggtc tcgatctcgt cgtactcgaa ctggcggccg
      541 gcgacgatct tccaggtgct ccagtcgcag gcctggcagg cgaacttctt gtagttctcc
      601 ttcaccaccc cgccgcactt cggacacggc gtctgcaggg tgacgaattc gccgggcacg
      661 gtgtcggact cgtagcgctt ggcgcgctcg accacctcgc gcgtcatctc ggcgatctcg
      721 ttcatgaagg cttcgcggct gagcttgccg tgctccatct gcgcgagctt gtattcccag
      781 ccgccggtga gctcgggcga ggtcagcgcg gtgacgccga ggcccttcag cagcgtgatc
      841 agcgagaagg ccttggcgct cgggatcagc tcgcggccgt cgcggtggat gtactgctcg
      901 ctgatcaggc cctcgatgat ctgcgcgcgc gtggccgggg tgccgaggcc acgttcggcc
      961 atcgccgcgc gcagctcctc gtcgtcgacc atcttgcccg cgccttccat ggcggagagc
     1021 agcgtggctt cgttgaagcg cgccggcggc ttggtctgca gcgacttgac cacgatgtcc
     1081 tcggtggaca ccgtctcgcc ctgcttcacc gccaccagct gcggggcgcc gccgtccttg
     1141 gcgtccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcct tctcgtcgtt
     1261 ggcggcttcc ttgccataca cggtcagcca gcccggattg accagcacct tgccctcggt
     1321 cttgaaggcc tcgccctcga cgcgggtgat gcgggtggtg atctggtact cggccgcggg
     1381 gtagaacacc gccaggaagc gcttggtgac caggtcgtag atcttgtgct cggcgtccga
     1441 caggctcttc ggcagcgccc cggtggggat gatggcgaag tggtcggaga tcttggcgtt
     1501 gttgaagatg cgcttgttcg gccgtaccca gccctgcctg gtgatctcgt tggcgaaggg
     1561 cgcgtactgg tcgggcaggg tgcgcatgac ctcgcccacg gtggcgagat agtcctcggg
     1621 cagcgcgcgc gcgtcggtac gcgggtaggt cagcaccttg tgcttttcgt acagcgcctg
     1681 cgccagttgc agggtcacgc gggcggagaa gccgaagcgg ccgttggcct cgcgctgcag
     1741 gctggtgagg tcgaagagca gcggcgacag ctgggtggag ggcttggcct cctcgctgac
     1801 cacgcccggc ttgccttcgc acttggcgcg gatggcctcg gcctgggcct tgtcccacag
     1861 gcggaaggcg gtggcgtgct cgtcgccttc cgggcgcttg aacttctcgt cgaaccagcg
     1921 cccggggtat tcgccggcgg cacagccgaa gcgcgcctcg agttcccaat agtcgcgcga
     1981 tttgaacttg cggatgcggt cctcgcgctc gaccaccatc gccagcgtcg gcgtctgcac
     2041 gcggcccacg gtggtgaggt ggaagccgcc ggtcttggag ttgaacgcgg tcatcgcgcg
     2101 cgtgccgttg atgccgatca gccagtcgga ttcggcgcgg cagatcgcgg cgttgcgcag
     2161 gccttcgacg tcctgcgcgg cgcgcaggtg ggcgaagccg tcgcggatcg cctgcgccgt
     2221 catcgactgc agccacaggc gctgcatcgg cttcttcgtg ccggcgtgct gggcgatgaa
     2281 gttgaagatc agctcgcctt cgcggcccgc gtcgcaggcg ttgatgaggc cggtgacgtc
     2341 cttgcgcttg atgagtcggg tgagcagctt gaggcggtcc tcggtcttct cgatcggctt
     2401 gaccgcgaag tggggcggga tcaccggcag atgggcgaag gtccatttgc cgcgcttgac
     2461 ctcgaattcc tcaggcaggg tgagctcgag caggtggccg acggccgacg acaatacata
     2521 ttcgtcggac tcgaagtagt ccttctcctt ggtgaagccg cccagcgcac gggcgatgtc
     2581 ctgcgcgacg gaaggctttt ccgcgatgat cagttgcttg ctcatgctcg acggtcaacg
     2641 agggtcgcgc cgcgagggcg cgtgggttcg gaagcggcgg atgataagga cggcccgccg
     2701 gaacaggcaa gccgagcggg tggaccggtc agtgcaccgt gggttcgtag gcccagtcgt
     2761 cttcctcggt cagcaactcg tccaccatca gggtgtcgat cggctcttcc tgctgccaca
     2821 gcaccatcag cacgatgacc ttgagccggt tgagcgtgat gttgaagtcg gccagcgcca
     2881 tcgcgcgttc gatgatcagc tcgcggtggg cggtgccgag cacgccggcg ctctcgagca
     2941 ggctcaggaa gccgcggcag cgaccgtcga gcagggcctg ctcttcctcg gtatagatgc
     3001 gcaccgaccc ggcctgcggt gcggtgccgg ggtggatgtc gcgggcgacg cggcgcaggc
     3061 cggcgagcca ctccagcgct tcggagatgt cgtcctgctc gaagcccgcc gcggacagct
     3121 tgcggttgag cagctcccct tccgggcagg cgtcggcgtg gatgtagctc tcgaagaggt
     3181 atacgaggat gtcgaacaag gcggcctctc gtggatcggc aggtgagacg gaagggccgg
     3241 gcccccgctg gggcatgggg acggcggctc gcggccgctc tttcaggtga tgcgctggaa
     3301 gcgtccgccg ggcagcttgg caaggcgccc ttccagttcg agtggcagca ggatggcgta
     3361 gagggcatcg accgtcaagc cgcagcgtgc ggcgatcgtg tcgaggtcca ccggatcgtg
     3421 gccgatcgcc tcgagcacgc gtgtgcgctc gccatcgagc gcgagcgcgg cctggcgagg
     3481 gggtgcggcc gggacggcgg gtgccgcgcc gcgactccgg cggtccttga cggatgcggc
     3541 gaccggcgct ggccagccga ggcggccgcg cagttcctcg acgacgtcct ccgccgtctc
     3601 gaccagcttc gcgccgtcgc ggatcaggcg atggcagccg cgcgacagcg gcgagtggat
     3661 cgagccgggg atcgcgaaca cctcgcggcc ggtctcggtg gcgaggcgcg cagtgatcag
     3721 cgagccgctg ccgagcgccg cctcgaccac cagtacgccc tcggccagtc cggcgatcag
     3781 gcggttgcgg cgcgggaagt tgtgctgcag aggcggcgtg ccgaggggaa actcgctgac
     3841 caccgcgccg gcagcggcga tctcgcgcgc gagcgcggcg ttgcgcgccg ggtagatgcg
     3901 gtcgatgccg gtgccgatca ccgccaccgt tccggcccct gtgctgccgg cggcgagcgc
     3961 gccgcggtgt gccgcggcgt cgatgccgag cgccagtccg ctgacgacga cgaggccttg
     4021 ctgggcgagc gtgcgcgcga aggcctcggc gttggcttcg ccggccgcgg tcgccgagcg
     4081 tgcgccgacc agcgcgatgc ccgggcgcga tagcagcgcg ggctcgccct tgacgtagag
     4141 cagcaccggg ggatcggcga tgtcgaagag ttggcgcggg tagtcggcgt cggccagggt
     4201 caggacgcgg ttgccggcct cgctcgccca tgcgagcgtg cgctcgatgt cttcgtgtgc
     4261 cggcacggcg agcacggcgt ccgccgcctg accgcccacc accgcggcga gcgcgccgcg
     4321 tccggcggcg aagatgtggc cgggcaggcc gaaggcggcg agcagggcgc gctggccctg
     4381 cgtgccgacg cccgggatgc aggcgaggcg cagccagtcg gcgaggtcgt cggcaggcgc
     4441 ggtcacgatc gggcggctgc ggcttgccgg tgccggatca gggcttgcgg agggtgtcgc
     4501 cgatggtgac cggaccgtcg gactccatca ccagcgcgta cgagacgcgc tcgaagacgc
     4561 ggaacacgaa ggccacgccg tagcgctttt ccggcaggcg gaagctttcc ttgccgtcct
     4621 cgccgcggta ctcggcgaca ccgcggttgc gatggagcgc gagcacgtgg ccgcgttcga
     4681 ggccgtcctg ctcgccgacg ttgagcgcga ccacgttgag gcggccggtc tcgctgaccc
     4741 cacgatagat ggagaggatc ctgccctcga cctcgaagtc cggtgcatgc ggggcgtagg
     4801 aaaacacgct cggcggctcg ctcggcatca tcaggtcgcc ggtgccgatc tcctcgaccg
     4861 cggagacgat ctccagcgtc gtcggttcgc cgcgctcggt gacgcgggcg gtgccgagat
     4921 aggcggcttc ccaggcgatc gcctcgcgcg tctgcgggtc ttcgagcgcg cgcgcggggc
     4981 gatagatgtg ccagatgtcc gcgtcgtcgc cgatattctt ggcgaagacg gtgtcgccgg
     5041 tgccggtcag cacgcggctg gtctcggtgg cgacgatggt ggccgagccg tcgaggcggg
     5101 gctggtcgac caccagcgga cggttcagga agggctcgat gatctgcagc ggaatgctcg
     5161 gcagggcctc ggcgatcggc tcgctatgga ccttcggctg caggcggccg tcgcgggtgc
     5221 cgccatcgcc gacgcgccgg cccacgctga gccatggccc gctgcggtcg agcaggacga
     5281 cctggccagg atagatgaga tgcgggttgc ggatttcgtc gcggttcagg cgccagacct
     5341 cgggccagcg ccaaggctcc tgcaggaagc gcccggagat gccccacaag gtgtcgccgc
     5401 gcaccacggt gtagctgtcg ggggcgtcgg gggcgaggcg cacgttcgac tgcgcgtgcg
     5461 cggcagggcc cagcaggctc gcgaaggcga cgagcagagg gaatataatt cgcatcattg
     5521 gacgcatccg atcgggacct cgcgtgccgg gttcgacccg gaacgctggc tatgcgccgg
     5581 ggtcgaagca acgaaccgcg cagtgcgcgc gtgctgcata ccggtcgcgg atattccggc
     5641 taaatacatg ccgaagcgtg tgaaaacgct tgaaattctg cactcaaagg cttaacccag
     5701 caaggtattc atggctctcc tacccattct ccgctacccc gatccccgcc tccacacggt
     5761 cgccgcgccg gtcggccggg tcgacgacga aatccgcagg ctgatcgccg acatggccga
     5821 gaccatgtac gaagcgcccg gcatcgggct ggccgccacc caggtcgatg tgcacaagcg
     5881 catcgtcgtc atcgacgtgt ccgaggacaa gtccggcctg atggcgctga tcaaccccga
     5941 gatcctggag cgttcgggcg aacaggtgtg cgaggaaggt tgcctgtccg tacccggaat
     6001 ctacgagaag gtgtcgcgcg ccgagcgtgt gaaagttcgc gccctgaacg aaaagggtga
     6061 atccttcgag ttcgaggccg acggcctgct cgcggtatgc gtgcagcacg agatcgacca
     6121 cctcgacggc aaggtcttcg tcgaatacct gtcgcagctc aagctcggcc gcatcaagtc
     6181 caagctggcc aagaaggccc gcatcaccgc gtgatggccg ccaccgcatc cggggccggc
     6241 ggcctgcgcg tcgccttcgc cggcaccccc gaattcgccg cgcaggcgct cgacgccatc
     6301 ctgaaggccg cttacgcggt cccgctcgtg ttgacccagc ccgaccgccc cgccggtcgc
     6361 ggcatgaagc tcagcccgag cgcggtcaag cagctcgcgc tcgcccacgg catcgaggtc
     6421 gaccagcccg agaagctgcg caccgaagag cagcgtgccc gcctcgtcgc ctgcgcgccc
     6481 gacgtgctgg tggtcgccgc ctacggcctc atcctgccgc ccgcggtgct ggcgctgccg
     6541 cgcctgggct gcatcaacat ccacgcctcg ctgctgccgc gctggcgtgg tgcggcgccc
     6601 atccatcgtg cgatcgaggc cggcgacgcc gagaccggca tcaccatcat gcagatggac
     6661 gagggcctgg acaccggccc gatgctgctg cggcgcgcgc tgccgatcgc cgcggacgac
     6721 accaccgcca gcctgcacga ccgcctcgcc gtactcggcg gcgcatgcat cgtcgaggcg
     6781 ctcgccgcgc tcgccgccgg cgcgctggtc gcgacgccgc agccggcgga gggcgtcacc
     6841 tacgccgcca agatcggccg cgccgaggcc gacatcgact ggagccggcc ggcggcggac
     6901 atcgagcgcg ccatgcgtgc cttcgacccc ttcccggggg ccgtgtccac cctgcgcgac
     6961 accgcggtca agtgctggaa ggcacgggtc gtggccggcg agggcgagcc gggccgggtg
     7021 ctcgcggtcg atgcggacgg catcgtcgtg gcctgcgggc gcgacgcgct gcgttgcacg
     7081 gtgttacagc gcccgggcag caagcgcctg cccgcggggg aattcctgcg cggtttcccg
     7141 gtctctgtgg gcgaacgctt cgcgtctgcg gcgtgagcgc gcggcgcgtc cgtggcggcc
     7201 ttgaaggcgg tcgcggacgc cccaagctac actgacgaat caatctctcg caggaaggat
     7261 cccgcaatgt tcggaaactg gatcaagacc tccatcctga tggccggcat cgtcgccttg
     7321 ttcggggcga tgggcgcggc catcggcggc cagcagggca tgctgatcgc gctcgtgatg
     7381 ggtggcgcga tgaacgtctg ggcctactgg ttctcggaca agatggtgct gaagatgtac
     7441 aaggcgcgcg aggtcgatgc cgcctcctcg ccctacctgt acaacatggt ggcggagctc
     7501 gcccgccgtg ccgagctgcc gatgccgaag gtgtacatca tcgacgaggc ccagcccaac
     7561 gccttcgcca ccggccgcaa ccccgacaac gccgcggtgg cggcgaccac cggcatcatc
     7621 cgcatgctct ccgagcgcga gctgcgcggg gtgatggcgc acgaactggc acacgtgaag
     7681 aaccgcgaca tcctgatctc gaccatctcg gccaccatcg cgggcgcgat ctcgatgctg
     7741 gccaacttcg gcatgttctt cggcggccgc gagggcgagg agcgtcccaa ccccatcgtc
     7801 tcgatcgcga tgatgatcct cgccccgctc gccggcatgc tgatccagat ggcgatcagc
     7861 cgcacccgcg agttcggcgc cgaccgcggt ggcgcggaga tctccggcga tcccgccgcg
     7921 ctcgccgccg cgctcgccaa gatcgacgcc tacgcgcgcg gcatcccgat gcacaccgcc
     7981 gagcgccacc cggagaccgc gcagatgatg atcatgaacc cgctctccgg cggcggcctg
     8041 cgcgggctgt tctcgacgca cccctccacc caggagcgca tcgcgcgcct gcagaccatg
     8101 cgctgaggcc tgcccgacgc tcagtccgac gcgaaggcgc tgcggatcag ggcgagcagc
     8161 tcgcccggcc gcaccgcctc gccgaaggca ccggcgcgca gcaggcactc gcgccgcgcc
     8221 gcgcgacgcg agggatcgaa ctcggcgtga tagaagatga ccggcggtcg ccgcgggcgt
     8281 gcggcgagca tgcgtagcag ctcgagtccg gcgctcgatg cggtggcggc cggttcggct
     8341 cggtcccaat ccgacagtat caggtcgaaa ccttcctcgt ccgcatcgag gcaggcgatc
     8401 gcttccccgg tgctgcgcac gttcaccacc tcgacctgca gtttcgccag cgcggccagc
     8461 tcatggcggt tgttttccgg gcggtcgtcc acccagagca cgcgcttctg cgcgggcatg
     8521 tcgatcagcg cctcggccag gccctccgcc tcgccgctgc cgatgccctg cacgcccttg
     8581 atgcgcgccg cgagggcgag ctcgtttgcg aacgcggcga tggtgcgctc gagcagggcg
     8641 tcggtgcggg cgtcccgggt cgcggccatc ctgggcgccg gacgcgccat ttcgagcgtg
     8701 cggacaggct cttccggaag cggcacgctt gcgaccgccg taccgaaacc gctcgcgcgc
     8761 ggtacggacg gcgcctccgg cggggctgcc ggcggggctg ccgggggcgg tgcgctcgcc
     8821 cagcgctggc ggatggcctc gaccgcgcgc cggatgccct tggcgacgtc ggtccaggcc
     8881 tcgtcgcggt tgggccacga ggtcaccggg cgcaggtcgg tgggcaagcc ctgcaggtgg
     8941 gcgaagggcg cgtcctcgag atcgaccgcg cgcagcagca ccgggatcac gctggcgtcg
     9001 ccgcgccggg cgcgttcgat cgcggtggcg agctccttgc cccagatata gtcggagttg
     9061 aggaagtcca tgctcaccag cagcaggatc aggtctgcca tggcgagctg ctcgtcgatc
     9121 gccttgtccc actcctgccc ggggacgatg ccgcggtcgt gccagggacg gatcacgccc
     9181 ttgcgccgca gcagcgcgag gtggccgtcg agctcgtcgc gcagcttttc gtcctcgtgc
     9241 gcgtagctgt agaagagatc caccggttcc atcacgatgc tccatcgggt tgcggcctga
     9301 tcttcaccat agatcaggcc ggctccgtct gcgtggtgtc gggacccccg cctcgcccga
     9361 gcccttcccg gcccgcttcc acggcgcact tcggtgcgga gcctccgcgc gcggaccaga
     9421 acgcctgcat gcatcggctt ggtgtaggct agcggtccca ccagccggcg acgcaggcac
     9481 gtgccggcgc aaccctcacc tgaaggagat cgacatgagc aaagtcaccc ttggcggcaa
     9541 cccgatcgac gtggcgggcc gtttcccgca ggccggcgag gcggcgcccg ccttcaggct
     9601 caccggcgcc gatctcgccg acgtgggcct cgaggccttc gccggcaagc gcaaggtgct
     9661 gaacatcgtc cccagcctgg acaccccggt gtgcgccacc tcgacccgca agttcaacgc
     9721 cgaggccgcc ggcctcgcgg ataccgtggt gctggtcgtg tccgccgacc tgcccttcgc
     9781 cgccaagcgc ttctgcgaga ccgaaggcct gaagaacgtg catacgctgt cgaccttccg
     9841 caacccgaag ttcgcgcagg actacggcgt cgcgatcacc tccggcccgc tcgccggcgt
     9901 caccgcgcgc gcggtcgtgg tcgtcgatgg caacgacaag gtggtgtatt cgcagctcgt
     9961 gcccgagatc aaggaagagc ccgactacgc cgccgcgctc gccgcgctga agtaagccgc
    10021 cgtgccgcgg gcgtcgccgc cgggcgcggt ggcgacgctc agcgccggga cgagccctgg
    10081 ctggccgccg ccgggcgctg cggctcgtcg aagcggctgt cgtcctcgag cgggaacacg
    10141 cccagcccct gggtggccag tcgcaccgtg gtcggcccgc ggtcctgcga cagcttgaac
    10201 agctgctcgc ccatgcgcac gatgcagccc tcgaacatct cgcgctccgc attcacccgt
    10261 gcctcgcggg tgagcgcgag gcggtgctgc agatcctcct cctcgctgcg caagctctcg
    10321 atctcgcccg acagcgcgct cgccgtctgc tcggcacgtc ccagcatctc gggtgtcacg
    10381 cgatcgggat ggcggtcggc gaaggtgagc atcttgccga tctcgagcaa gcggttctcg
    10441 agtgcgtcgc gcgcctcggc cttctcctgg atggcgtgtt ccagcccgtt gtccatgccg
    10501 atctcgagct cggtgcggat gcggctgttc gcgccgatcg tgcgcgcgtg gatggacagg
    10561 ctggcgcggc aatggccgcc gacgatctgg ccgcgcaggc gcttgcctac gcggatgtgg
    10621 ttgcgtgcct cgagctggca ctgcatggcg acgtcgtcga tgaagatcga gtcgcccgcg
    10681 ctgatgcgcg cctgctgcgc gtaggtggcg cagaagctgc cctcgcagcg gatcgcgtgc
    10741 gcgctgtaat ccttgccggc ggtcttgccg cccagcccgc cgagcacgcc gcccttgacc
    10801 acgatgctgc cgccggcctc cagcgtggcc ggctcgacca cgccgccgac ctcgatgtcg
    10861 ccgctggcgc gcaccgtcat cccggcctgc acatcgttgc ggatgcgcac gctgccgtcg
    10921 aagtcgatgt tgccggtggc gaggttgacc gcatccacgg tgaagatcgg ctccaccatc
    10981 gcgccgttgc gcacccgcac cggctggccg tcgcaggcag ccacgagcag ctcggggtcc
    11041 tcgggcgaga tcgcggtgcc gcgcaggccg ggggcgaagc gctgctcgcg accgcgccgc
    11101 gccacgatcg gccggccgta cacattgacg ccgtcgactc cgggctgcgg gggatggcgg
    11161 cgcatcagcg catcgccggc gtggacgacg aggatctcgc cgaggtcgcg gtagtcggtg
    11221 cggccgcagg ggcgcacgct gggtacgcgc tcgcgcgtct ccggcagcag gtactcgagc
    11281 cagccgtcct tgcccggttc cggttcccgg ccgcgcgcga tggcgaggtc gtcggcctga
    11341 ccgtcggcga tggcgcggtt catctcctcc agcagcaggc cctcgacgat gccgcgcgat
    11401 tcgatcaggg cgagcagttc ggtcttgctc gccggcttgc cacccttggc cggcacgatg
    11461 ctcaggcgtg cgaccagtcc gtccagcgag actccgatct gcatcgacgc atccacgcac
    11521 tgcgcgatct cgaccggcgc caccgcctcg ccggcgttgt actgggcgat caggcggcgg
    11581 atcgggtcgg ggcggatctg caggtcggcg tagcccgcgg cttcgagccg tcggcgcagc
    11641 cacaaggcgt cgatgcgcgg aaaatgcggg tcatgggcga tgctcacgct caggacgcgc
    11701 gtgagttcgt cgaacgacac ctccagttcc gggccttccg tgggctgctt gtccatgttt
    11761 ccagtgcgat ccgatcagca aggtccgcgt gcgcggtcgg gggattgtag ccgcgcgcat
    11821 attgttggtg ccaatggggc gaacccgatt cgacgcccgc ggccgtgtcg cccgtgcaat
    11881 gctcaggcgc ccagtgcgcg ggcgatgaag tgcctggcca cgcgcgggcg cggcaccttg
    11941 tcctcgaacc aggcgcgcac gtcggtgtcc aggccgatgc cgtgcatgta gttgtatagc
    12001 gccttgttga gcgccttgcc gaggcggtcg tggtccaccc cggtggggtc gatgaagccg
    12061 acgtcgttct tcgcgaacgt gatctcgggc agcgccacca gggtgacgcc gaactcctcc
    12121 gggttcctgc ccaccggaga atgcacggtg catgcgaaac gatggaagaa gcccgactgg
    12181 atgcagccgg cttcgaagag ctggcgcacg tattcgagcg cgtcgacggt gtcgtgcacc
    12241 gtctgggtgg ggaagccgta catcaggtag gcgtgcacca gcaccccggc cgcggtgaag
    12301 gcgtgggtga cgcgcgcgac ctgctcgacc gaaacgccct tcttcatcag ctgcagcagg
    12361 cggtcggagg ccacctccag cccgcccgag accgcgatgc agccgctgtc ggcgagcagc
    12421 tggcagagct cggggctgaa ggatttctcg aagcggatgt tgccccacca cgagatcgcc
    12481 aagccgcgcc gcagcagctc ctcggcgagc gcacgcaagg ccttgggcgg cgcggcctcg
    12541 tcgacgaagt ggaagccggt ctggccggtc tcggcgatga tcgcctcgat gcggtcgacc
    12601 agcaggccgg cggcggcgcc gtcgtagcgg ccgatgtagt cgaggctgac gtcgcagaag
    12661 ctgcacttct tccagtagca gccgtgggcc acggtgagct tgttccagcg cccgtccgac
    12721 cacaggcggt gcatcgggtt gagcatgtcg agcaccgaca ggtagccgtc cagcggcagg
    12781 ccgtcccagg tcggggtgcc gacctcggcg aagggcacgt cgggctcggc gaggttgatg
    12841 tagcgcacct ggcccgtatc cgcatcgcgc acgaaggtcc gcaccagccg ggatctgccg
    12901 cgccggccct gcaggtgctc gagcagcgcg agcagcgggc gctcgccgtc gtcgagcgtg
    12961 acgtagtcga aatggtcgaa cacccgtggc tcggcgagct cgcgcagctc ggtattgacg
    13021 aagccgccgc cgagcacggt gacgatctcc ggatgcgcgg ccttgatcgc ctgcgcgatg
    13081 cggaaggcgc cgtacaccga gcccgggaag ggggtggaga ccagcaccac ctgcggcgag
    13141 tggcgggaga gggcttcgag ggtcaggtct ttcaaatacg cgtcgaccag cgtcggtgcg
    13201 gcggcaagcg cggcggcgag cggctcgaag gtgggctggc tcatcgccag cgtctcggcg
    13261 tagcgcacga actcgaagcg cgcatccacc gcgtcgcgca ggatgtcggc gagctcgttg
    13321 aggtacagcg tcgccaggtg gcgcgcgcgg tcggcgaggc cgagggcgcc gaaggcccag
    13381 gcgagcgggt cgccgccctc ggggtcgtcc gggtcgacgt aggcgtccac cgcatcgaag
    13441 cgcggcccct cgggcaggaa ggtccggctg gcgatgcggt gcgcgatggt cgggtcgcga
    13501 ccctgcagga aggcgatggt ggggtcgacg gtgtagcggt agcgcgcgaa gtggtcgaga
    13561 aagccctgta ccagcggcgt gcgccgcttc ttcggcagcg cctcggcgcg ggcgcggatg
    13621 tcgtccagcc cggcgggcga gagcaggcgc agcaccagct tcagcgccag gtcttcctgc
    13681 accgcatcca ccccgcgcga gcgcaggaag ccggtgaggt aggcggtgga cgggtagggc
    13741 gtgttgagct gcgtcatcgg cgggatgacg gagagcacgc ggatcgggtg ggcggagggc
    13801 ggcgcggcgg acatggcgga tgttgcgggc gaagggcgcg aggatgccgc agccgctact
    13861 ggaacgccac ctcggcgaag ctgcgcagct tgcggctgtg caggcggtcg accccttgtt
    13921 cgcgcaactg ctcgagcgcg cggatgccga tgcgcaggtg ctggtccacc cgctcgcggt
    13981 agaacttgtc ggccatgccg ggcagcttga tctcgccgtg cagcggcttg tcgctgacgc
    14041 acagcagggt gccgtagggc acgcggaagc ggaagccgtt ggcggcgatg gtggcggatt
    14101 ccatgtcgag cgccaccgcg cggctctggc tgaagcggcg ctccgggctg ctcgacatgc
    14161 cgtgcgaggg cagcagctcc cagttgcggt tgtcggtgct ggcgacggtg ccggtgcgca
    14221 tcaggcgctt gagctcgtag ccggacagct gcgtgacctc ggccacggcg gcctcgagcg
    14281 ccacctgaac ctcggccagc ggcgggatcg gcacccaggg cgggagttcc tcgtcgagca
    14341 cgtggtcctc gcgcacgtag ccgtgggcga gcacgtagtc gccgagctgc tgcgtgttgc
    14401 gcaggccggc gcagtggccg agcatgatcc aggcgtgcgg gcgcagcacc gcgatgtggt
    14461 cggtgatggt cttggcgttg gccgggccga cgccgatatt caccatcgtg atgccggcgt
    14521 ggtcgccgcg caccaggtgg taggccggca tctgcggcag gcgcggcggc ggggtgccat
    14581 cggcgtcctc ggcctgcggc gcgaggtcgc tgcggcgggt gaccacgttg cccggctcga
    14641 cgaaggcctc gtagccgtgg ccggaggcgg tgtcggccat gagctcgtgg ccgaggcgga
    14701 tgaactcgtc gatgtagaac tggtagttgg tgaacagcac gaagttctgg aagaaggccg
    14761 gccgcgtgcc ggtgtagtgg cgcagccggt gcagcgagta atccacccgc ggcgcggtga
    14821 acagcgcgag cggatgcggt tcgccggggc cgggctcgaa ggtgccgttg gcgatgccgt
    14881 cgtccatggc gcccaagtcg ggcaggtcga agtgatcgcg cagcaggcgg cggcgctcgg
    14941 ggccgaggtg gccttccagg tagtcgtgct cgggcagggc gaaatgcacc gggatgggct
    15001 gcgtgctgct gcccacctgc aaggccacgc cgtggttctg caccagcagg cggaattgct
    15061 ccaggtagta gtcggcgaac aggtcgggcc gggtcagcgt ggtctcgaac acgcccggcc
    15121 cggcgacgaa gccgtaggac agccgcgaat cggcgcgcgc cacggtgtcg gtgcgcacgc
    15181 gcagcagcgg atagcaggcg cgcacgtggc ggccgatgtc ggcgccgtcg acgtagtgct
    15241 gcaggccgcg gcgcaggtgc tcgaccgcga ggtcgtagat ctcgtgcgcc cgggccagcg
    15301 ccgcggccgg atcggcgaag tcctcgacgt cgaagtgggg ggcgtggccg ttcatcgttt
    15361 gcgggtgctc gtggttcagg tgcgcgcgag caggcgctgg accgcctcga ggtgctcggg
    15421 ctcgttgtgg cacatgccct ggaaggcggc ggaaaggtcg aggaaatcct tcagttccat
    15481 gcgctgcgcc atcttcatca ggcgcttggt gaggcgcagg gccttgggag gctgggcggc
    15541 gaagcgtgcg gccagctcgt gcgcgcgggg cagcaactgc tcgggttcga ccacctcgag
    15601 cacgatgccg agcgtcttcg cctccgcggc gccgacgatg cggcccgaga gcgtgagctc
    15661 gaaggcctgc tggtagccga tcaggcgctg catgaaccag gcgccgccgt cgccggggat
    15721 gatgccgagg ttgaggaagg tctcgccgaa tttcgccttg gtcgatgcga tgcggatgtc
    15781 ggccatgttg gcgaggtcga agccggcgcc gatcgccggg ccgttgaccg cggcgatgac
    15841 cggcacctcg accgcctgca gcgccagcgg gatgcgctgg atgccctggc ggtagcgggt
    15901 ggcgacctcg gccacgtcgc cggcgaagtc gccgccgcgc gccgccatgt ccttgacgtt
    15961 gccgcccgcg gagaaggccg cgccttcgcc ggtgatgacg aggacggaga cctcctcgca
    16021 gcggttcacc cattcggcca cgccgacgat gtcgtcgatc agcgcggtgc cggtgagcgc
    16081 gttgcgcacg tcgtcgcgcg ccagcgtgag ggtggcgacg cgcttgtcga gttggaggcg
    16141 ggcgtcgtgg acggtgggca gggtcatcgt gcggtctcct tgtgggcggc ggacagttgg
    16201 gggccgcggt gcgcggcctg gggcagggcc tgggactggt tcgggtgctg cgttcagcgc
    16261 gggttacgga gcggctcggg caatttcagc atgcccgcat cgctgaagcg ggtgccgccg
    16321 agcagcccgc cggcgagccg cccgcgcagg gtgtagggca cctcggtctt cggttcgcct
    16381 tcgatgacgc cgagcgcctg gcgcaccacc gcgatcgccg agaccgacac cgggatctcg
    16441 acgagcgcct cgccgaagcg cggcacgctg cccttgcggt cgctgacgcc ggtggcgaag
    16501 ggcttgccgt cgaggtcgag ctcgagggcg aggccgtcga aatcgatcgc cgcctcgttg
    16561 gggttctgca cgcgcagctt cactgcgaag cgcatctcca tgcccgcgcc cggcaggggt
    16621 tcgagcccca cgacgttgat gcgcaccggc tcgcgctgga tcaggctggc gcagccggcg
    16681 agcagcaccg atgcgagcac ggcgagcagg ctgcgggtga aggacgaggc agacgggcgg
    16741 acagcaaggg acatggtgcc gactccgggc aagggcgcgc gctctgcggc cacggtgggc
    16801 gagtgtggca ttcattctaa ccgcgccgtg caaggaagct ggcgcagtcg atcggcgtgg
    16861 gacaaagcgt ctccgccttt tgaattcgac ccgcattggg gctatggtga aagcatcggt
    16921 ggtgactgcg gtgccggtcg gttgtgcgcc ggtgctcgtc cccccggttg tccgcgcagc
    16981 cttcttccct ggagtcgagc atggtttccg ccccgcgcgc gcatcctggc gtcgcgcccg
    17041 cgtgtttcgc cctcgtgccg gccgcggcga cagcaggcgc ggtgttggct cgctatggcg
    17101 agctggcgct cgtcgaggtc gaacgtgtgc cggtcacggc ctgggcgagc tggacggatc
    17161 tcgatccgat ccgggtccat ggcgtgcccc gcattcttcc gtccggatgc ttcgaaccgg
    17221 gagcggggcc gcgctgcatc ctgcgcttcg ccggcccggt attgccagcc tggcgcgaga
    17281 cgctcgccac gctgggctgg acgccacgct tcgactgtcc gcccagcggc ctgtgtgcgg
    17341 acgtcggtgg cgatcttgtc gccctgcgtg ccgcgcttcc tgccttgcgc gcgatcgctc
    17401 cctacgcggc ggaggactgc gcgcgcgcgg gcgcgggcgg ggtggcggcc ttgccggaca
    17461 ccgtcgatgc ggtggccttc agcgtggaag accgcgtccg cctcgaaggc gagctcaccg
    17521 ccgccggcct gcaggtggtc gcgagcagcc cctacaagct gcgggtgagc ggcgatgcgg
    17581 gtgcggtcgg ccggctgcgc gacgcggtcg gggtgaagat tgccgatccg ggcaccctgc
    17641 cgcgcacggc ctcgctgtcc ttgcggctcg cgatcggtgc gaccgccctg ccggagagcg
    17701 cgggcggcgc gactggcgcg cagggcggcg agggcgaatt gatcgcgttc gccgataccg
    17761 gcctgggaac cggtgcgacc gatgcgccgc atcccgattt cgccgggcgg gtgcaggcga
    17821 tccatagctg gccgctcgcg cccggatggt cggccttcgt gcgcgctccg ggcgcggacg
    17881 acggtggcgc cgatcgtggc agcggccatg gcacccatgt ggcggggctg gctgcgggcg
    17941 acggcggctt gagcgccggc aggctggtgg gcgtggcgcc gggtgcgcgc atcctcatgc
    18001 aggcgatcga gcagtacgtc gacccgctgc cggaatggcg cagccggctg cagcccggct
    18061 tctacctcgc cgggcggccg ctcgacctgc gcgagctgct cgaacaggcg cgcgccgccg
    18121 gtgcggcgat ccacgtcgac gcctggggcg tggaggctgc gggcgcctat accgacgaca
    18181 gttgggagtg cgaccatttc cttcacgccc atgccgagat gctggtgatc gtcgccgccg
    18241 gcaacgccgg ccgcgaccgc aaccgcgacg gggccgtcga tgccggcacg ctgtacgcgc
    18301 cggcaagcgc gaaaagcgtt cttgccgtcg gtgccaccga gggaccggat gccggcatcg
    18361 gcctgcgcgg cgggtgggcg gcgctcgatc ccgctggcac gcgctttccc gatccggcac
    18421 agcgcggcgc gtccgtctcg ggcgacgccg agcggctcgc gctgctgtcg agctgcggtc
    18481 ccacgctgga cggccgcatc aagcccgacc tgtgtgcgcc cggcaccaac ctcgccgccg
    18541 cgcgcagcac ggcgagttcg gcacggggct ggggcctggc caatccgctg ccctggtacc
    18601 agtacctcgg cggcacctcg atgtcgaccg gggtggtcgc gggtgcggcg gcggtgctgc
    18661 gccagcgctg gcggccggtg ctcggcgagg cgccgggcgg cgccgcgctc aaggcgctgc
    18721 tgatccacgg cgcacgggcg gtgcatcggc gcgacggaca gggcgaggag gaccgccagc
    18781 gcgcgggctt cggtcgcctg tggctggccg gcagcctgcc cggtcccgac gggccggtgc
    18841 tgatcgagga ccgcgtcggt gtggcgaccg gcgagcgccg ccgcatcgac tgcacggggc
    18901 cgggacggct gcgcgcggtg ctgtgctggt acgacgcgcc gggcgaggcc ctgatcaacg
    18961 acctcgacct ggtgctgtgc gatgccggcg gcagcgtgct tgcctggggc aaccacccgg
    19021 ccggcgcggt cggcagtccg gaccggcgca acaccgtcga atgcatcgac gtggcgctcg
    19081 gcgagggcga gtggatgctg gaggtgcgcg gcgtgaatgt gccgctcggg ccgcagccgt
    19141 gggcgctggc gacgagtccc ggcgtcgtcg tgcgggccac ccccgcggcg ccggggacgc
    19201 cgacggtgcc cgcggatccg ctgcccgcgc cgcgttggcg ggtcgacctg ccgtggacgg
    19261 cggtgcgcgg cgtgggggcc agacgcgcga tcgaactcga gcgcacgctg cccggcgggc
    19321 tcgccgcact atgcgccggt gccccggacg aggatgcgct ggccttcctg cccgcggcct
    19381 tgcgcgcgca gatccgcagc gtggccgcct tcctcggtgc gccgccaccc gccgcggcgc
    19441 gactcgcgct ggcgcatccg ctccacgccc tgctctccgg cgcacccgag accgtctcca
    19501 ccgtcgcatg gcaggccctg gccgccgcgc tctctccctt gctcgaagcg ctcgaccgca
    19561 acgtcgcccg cgaggtccgc ggcgagcgct tgttccgatg aaccactcac aaggagtgca
    19621 tcatggctga caacaccgaa aaaccgggca agggcaagcc gacccggacc caggccggcg
    19681 cgacgtcgcc ggctgccgcg ctgcccgagg gcagcatcct ctcggcgggt gcgctgatgc
    19741 ccggtgcggt tggcggcgcg ctcgccctcg gggccgacgt ctccggcgaa ctcgccgagc
    19801 tcgcgcccag cttcggcgat atcctggcct cgatcggccg cggggtcgcc gattcgcaga
    19861 ccgccctcga tcgtggcctg gtcgacaccg cgaccaccct gtcgcagtcc aacatcacgg
    19921 tggtcaccga cgtcgtgcag gagttgaacg acgacggcct cccggtcccc agcgcgaccc
    19981 agctcgtgac ccagaacgtg tcgctgatca acttcgtccg tcccaccgcg catcagtgga
    20041 gccacgtcgc gctgcgcatg gacatgaccg tcggtgcggt cgacaacgag cgcggcatca
    20101 gcgtcaacag caagaccaag agcgcgcggg cgaccaatgt cggcctcttc ttcggcttcc
    20161 tgggcatcgg cgtgtcgtcg ttcaaggaga aatcgacctt ccgttccgag gagtcggact
    20221 tcgaatcgaa ctgggcggag gggcgcgtgc tgatggacgc cctgctgcag ccgcgcaacg
    20281 tcgacggatt cgacccgccg gccgaggtct ccatcggacc gcagatcttc atggcgctgg
    20341 gcagcgtgca ggagacgctg aactcgggcg tcgtcaccgc gcgctccacc gacatcgtga
    20401 tcacggtgcg caaggccaac ggcgcggtga atccgaccgt gacgatcgac gtcgatcccg
    20461 gccccttcga cctgtccttc gcgaccgccg acggcttcac cggcaacacc acgaacgcgg
    20521 ccgggcagtg ccggatcacg ctgtcgcgca acatccccag cccgctcttc cagcgccgcg
    20581 tgcgcggcac ggtgaccgcc acgctgggcc agatcgaacg cagcacgacg gtgtcgctct
    20641 gagcgccgcc cgccacagga gaaaccatca tgcctgatct acttcctccc cttgcccgag
    20701 cgctggccga ggacgccaac gtctccgacg aactgcgcgg tgccggcatc gccttcggca
    20761 cgctggtcga acgcaccggc gcggccgtgg ccgcgaccca gctgcagctc gacacctcga
    20821 gcgcggcgac ggcctcggcg ctggccgcca cgctggtcaa cgtgatcgcg gtgcgcgagc
    20881 aggtgttccg cgacgacggc acgctcgacc atgtcaccaa ccacgtgcgc ccgctgccgc
    20941 tgatcagctt catcgatccg gtgttctacc aatggaccag cgtgcgcctg cagggcgagt
    21001 tctacgcgcg cgagttcgcc gcgtcctcgg agaccacgag cgcgtactcg ggctcgaatt
    21061 tcgggctctt cggcagcggc gtgtcgatgt tcttcggccc cggcatggtc accagcggga
    21121 gcggatccgg gtcgagcacc accacgaccg acgtgtccac cgacgtctcg ctcggccgca
    21181 tccgcgccag tgcgctgctc gagccgcgcg acgacgtcgg catccccaag cccaaccagg
    21241 tgatccgcgg gccgcggctc gcgctcatcc agggcgagat cgccgacgtc atggatggcg
    21301 gcgatctggt cggacgcacc atgagcgtgc tgctgcagta cagtcgccgc aatggcgcgc
    21361 cgatcgccgg caaggcgctg tcggtcgaga gctccggcgt gccctggcgg catctcggca
    21421 gcggcgtcac cgatgccgcg gggcagctcc agatcgagct gaggcgtgac ttcctcgacg
    21481 aggacgccga cacggcgccg gccagcttcg tgatcaccgc gcgcatcggc ctggtgagca
    21541 actcggtcac cgtcaccttc tgaacggtcc ggcgcggggg cgggaaggcc cgcctccgcg
    21601 ccggcgggag aacgcatgga caaggcgaac gtcagtgccg gcgaactggc cggccagcag
    21661 gccgtcgagc tgggcgaact gctcgcgcgc accgtagtct cggtcgccgc ggcgcaggag
    21721 gcgctcgacc gccacgccac cgatcgcctc gacgcgatcg agcgcggcga cgaggacgcc
    21781 ccccgggtgc cgccgctgtg gtacaccttc accgaggtga cgctcgaggt ggcgctctcc
    21841 gcatcggtcg agcgcgcgag cgggagcgtc ccgccgcgcc tcatgtcgcg ccctgtggac
    21901 gcgcgccagg tcagcctcta cggctaccag gccgcgagca gcctgatggt gcggctgcgg
    21961 ctggcaccgc gccagggcat gcccgacgtc tcgccgccac ccgcctgaat cgcccgctcc
    22021 tctcctcacg aacctgtcga tccgccatgc ccatcgatcc caaggacctc cgcaccgctg
    22081 ccggctcgct gctcgacccc cggctcgtgg acacgatgaa cctgaagccc gtcggcacgt
    22141 ccagcctggt gaatctggag ggcaggctgg gcgtcttcac cccggtggcg gaggacggca
    22201 gcggcgccga caacaccgag gcggtgatcg cggcgctgcg caaggagctc gtccgcctcg
    22261 agagcgagaa cgccgcgctg cgccgccagc tcgccgaggt cgaggcggcg cgcaagcgca
    22321 gcccggacga cttcgccagc gcggtcgccc acaccctgga caccctgcag cacaagctgg
    22381 cgcagaccac caacccggtg agccgcttcg cggtgcgcga ggtggcgatc gacgcccagg
    22441 tgacggtgga cgtgtcgccg ctgggcaccc tcgagtaccg cttcgtgcgc cccgaggacg
    22501 acatcgatcc gtccaagctg tcgcgcatcc ggctcgacct cgtgcccctg ccgcgcgagg
    22561 acgcgggcct gagcgccagc ccggagttca gcccgtgggc gggcgtggaa gacatcgtcg
    22621 gggtcggcga gacctggcgc ggcgcgctca accgccagca gatcttcacc atcgccgacc
    22681 tgctggtggc cgggtcgcgg ctcaagtcga gcgccgaact ggccgcgctg gtgggggtgg
    22741 atcggcagcg cctggccgag tggatgggtc atgcggagct gctctcggtg cgcacgatcg
    22801 agccgcgcgc ggccgacgtg ctgtggacgg tcggcatccg ctcgctggag acgctcgccg
    22861 gcgcggcggc ggtggaggtc gtcgagaaat acaacgccga ggtcgcccgc cgcgcgcaca
    22921 agagcctcgc cccgatcgac gcggcgcagg ccgcctcctg gatcgaggcc gcgaagaagt
    22981 accacggccg gccgcgcgag ggcggctgaa gctgcctcag gcgcgcacga gccagtcgag
    23041 caggccgtcc atcagcgcgt gggtcgcggc gaagtgcccg tcggcgtggc cgaggatcgt
    23101 cgcctcgtcc tggatccagc gtcccggcac cagctcgccg cggcagttgt cgacgatcgc
    23161 gcgcaggctg gatgcggtca tctcgaggtg gaactgcagg ccgatcgtac gcccgccgag
    23221 ctggaaggcc tggtggcggc acgccgggct cgacgcgagc aggcgcgcgc ccgcgggcag
    23281 atcgaaggtc tcgccgtgcc agtgcagcac ggtggtgcgc gcggggaagg ggaagcgcga
    23341 tgcggtgtct gcagcgtcgg gttccgctcc gctggcggcg acctcgaacc agccgatctc
    23401 cttgtccgcg ttgcgataga cgcgggcgcc gtgcgcgctg gcgatcagct gcgcgccgag
    23461 gcagatgccg agcaccggcc ggccgagcgc gaccacttcc gcgatgaagc gcttctcggc
    23521 gaccagccag ggcaattccg cctcgtcatt gacgctcatc ggcccgccca tcaccaccag
    23581 cagatcgacc gcggccgggt cgggcagcac cggcgactcc cagaagcggg tcgagccgat
    23641 gtccgcaccg cgcgcggcga gccagtcgcc gatgtgaccg gggccttcga agggaacgtg
    23701 ctgcaggatg tgggctttca tggcgtcctg gggtggcgag atggaacggg tggcctattc
    23761 tagaagcgcg agccgggctg gcgcaggaat tcggcctcct ccgggcttgt ctcgcgcccg
    23821 agcgcggcgt tgcggtgcgg gaagcggccg aagcgctcga tgatctcgag gtggcggcgg
    23881 gcgtagtcga ggtagccggc gaaggccgtg cgcagcgcgg gctcgaccgc ggtggcgagc
    23941 gtatcgaacc gacgcaccgc ctcgcgctgg tcggcgaggt tttcggagtg ctccagcggc
    24001 aggtacagga agacgcgctc gatcgggcgc agcccggcgt cgaggccgcg cgcgagcgcc
    24061 tccttcgccc agcgcagcgc gagcacgtcg aaggcgaagg ccgcggcttc gccgcgccag
    24121 atgttgcgcg ggaactggtc ggtcagcagc accagcgcca ggcgcccgcg cagcccgccc
    24181 agccaggtgt cgagctcccc gcccgcggcg cggcccacca gcggggcgaa gcgtgcgcgg
    24241 atctccgcgt cgaccgcggg ctgtttcctc caccatagcg ccgactggcg ctcgatgatg
    24301 gcggcctctg cgtccgcatc ggccgcgggg ccgaaccaga aggcgtggat cgtggcgggc
    24361 gtgtccatgc ggggctccgg gcggccgatc ggggtggggc aagcatagcc cagcccgctg
    24421 cgatgcggga gcgtcgggcg cgagggcgat cgctccaacg gactgttccg ctcccacgcg
    24481 gcaggcgatg agcgcctgcc acgccttcat gcaatcgccc tgctccggac gcctggttcg
    24541 caccccgtcc gcgcgggtgg cgcaataatg ccgccatgcc cagcgacacc gcctcccccg
    24601 ccccgctctc cgccctgcgt ggcctgctcc ccttcctgcg cccctaccgc gcacgggtgg
    24661 tgctcgcctt cgtgttcttg tgcctgggct cggcggccat cctggtggtg ccgctggcct
    24721 ttcgcgacct gatcgacgcg ggcttcggcg gcgtgtccgt cggcggtgcc gcgacgggcg
    24781 gggcggcgga cggcggcggg ctgtttggcg cgcaggggct ggacggacgt ttcgtcgcgc
    24841 tgttcggcct cgcagtgttc tgggcggcgg cggtggccgc gcgctactac acggtgtcgt
    24901 ggatcggcga gcgcgtcacc gccgacctgc gcagcgcggt gtatgcgcgc gtgctgcgcc
    24961 agtcgccgca gtacttcgag accctgcaga ccggcgaggt gctgtcgcgg ctcaccggcg
    25021 acacgacgct ggtgcagacg gtggtgggca gctcgatctc gctggggctg cgcagcctct
    25081 tccagttcgt cggcggcatg gtgatgctgg cggtgaccag cgtgcagctc ttcgggctca
    25141 tcttcgggct catggccctg ctcgcgctgc ccatcacgct gatcggccgg cgcgtgcgca
    25201 gcctctcgcg cgaggcgcag gaccgcatcg ccgacgcctc ggcgctcgcc ggcgagatcc
    25261 tcgcggccat gcccacggtg caggccttcg ggcaggagga caacgaggcc gcgcgtttcg
    25321 ccgcccgcgc cgagaccagc ttcgccgccg cggtgcggcg cacccgggtg cgcgcggcgc
    25381 tcaccgggct gatcatcgcc gcggtgatgg gcacgatcat cttcgtgctg tggatcggcg
    25441 cgcgccaggt gcaggccggc gcgctgtcgg tgggcgatct cggcgccttc gtgatctacg
    25501 ccgcgctggt cgcgggcggc gccggcacgc ttgccgaggt gtggggcgac gtgatgcgcg
    25561 ccgcgggggc gagcgggcgg ctgctggagc tgctgcaggc agagccggtg atccgggatg
    25621 cagaggcggc ggagggcgta gcggaggcgg ccggaggtgg cggttcgcga cgggcgccgc
    25681 gcctgcatga gaacccgcgc agcacgcgcc ctgcgggcgc ggcgcccgcc gcgaaccccg
    25741 cacccgattc ccccaccaag gcccctgcca cccaccccgc gatcgaactc gccgacgtcg
    25801 tcttccgcta ccctgcccgc cccgccgtag cggcgctcga cggcatcgac ctgcgcatcg
    25861 cagcggggga aaaagtggcg ctggtcggcc cctcgggcgc cggcaagacc agcctgttcc
    25921 agctcctgct gcgcttctac gaggccggcg agggctgcat ccgcatcgac ggccacgaca
    25981 tccgcgcgct gccgctgcgc agcctgcgtg cgcgtatcgc cctcgtgccc caggacccgg
    26041 tgatcttctc cgcgagcgcg ctcgacaaca tccgctacgg tcgccccgac gcaggcgacg
    26101 ccgaggtgat cgccgccgcg cgcgccgcgg ccgccgacga gttcatcgcc cgcctgcccg
    26161 agggctacgc aaccttcctc ggtgagcgcg gcacgcggct gtcgggcggc cagcgccagc
    26221 gcatcgcgat tgcgcgcgcg atcctcaagg gcgcccccat cctgctgctc gacgaggcca
    26281 ccagcgcgct cgacgccgaa gccgaggtcc tggtgcagca aggcctggcc gccgcgatgc
    26341 agggccgcac cacgctgatc atcgcccacc gcctcgcgac ggtgcagaag gtggatcgca
    26401 tcgtggtgat ggacgcgggg cgcatcgtcg agaccggcac cccggccgac ctgcgccgcc
    26461 agggcgggct gtatgcgcgg ttggcggcgc tgcagctgga gggctgaccg gagcctcccg
    26521 cagcgcaggc gcggacccgg tgcgctgcaa tcgcgactcg cgtcgnnnnn nnnnnnnnnn
    26581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26641 nnnnnnnnnn nnnnnnnnnn nnnnncgcag gcgcggatcc gttgcgccct gcggcgggca
    26701 cgcacgcgct gacgctcccg gatggcgccg gcgcttcacg gcagcgtgcg ttcgcgcagg
    26761 tagcgcggaa agcgcggcag gccggcgggg gtgagctcgc ggtagcggta ggtgacgagg
    26821 gcgccgagcg cgggcgggtt ggcgcgcagc gcgtcgctga ggccgctgcc gatgcggaag
    26881 cgccgcccgt ccgcgcgctc gacctgcagc gcgcccaccc tgccctgcag cctgcctttg
    26941 cccggcaggt agcccaccac gcgcgcctcg gcgtcgagcc agggggtgag cttgcgcagg
    27001 gcctcgctgc ggccgggctg ccagagggcg tcggcgcgat gcagcatgag gccttcgccg
    27061 ccacctgcga ggacctcgcc gagcaggcgc tgcaaggcgg cacggtcggg tatgcgctgc
    27121 tgcgggatcg cctgcaccca gggcgcatcg gcggcctcgg ccaggctgcg cagacgcgcc
    27181 agccgggtgt cgaagtcgcc ggcggcgccg ggcaattcga agagcatgta gcgcacctcg
    27241 cgccacagcg ggtctgcggg atcctcgcgg cgcagcaggc cggagagctc gtcgaagcgg
    27301 cggcggccga gccagagctc gccgtcgagg gcgaccggcg gcagcgcggc gaggaaccag
    27361 gccggcgccg gcaacgcgcg cccgctgcgg aaacgcagca cgctgccgtc ccacgacgcg
    27421 cgcacgccgt cgagcttctc gctcacccac cagcccgcgg ggtcgatcgc ggggtcgtag
    27481 cgcagggcga gcatggctgc gggcgcgtct gcgggctccg cggtctgtgc gaccgcgccg
    27541 gccggcagca gggcgagggc caggatcagg ctgcggatgc agctgcccgg cctgcgcagg
    27601 gccggtgcgg gaatgcgggt gggcggtctc acgggcgaac cctccagtcc gatcgcgggt
    27661 gtgggcgcct cttcgcccgt gccgcgccaa cgcggacaag gcttgcgggc aagcccctcg
    27721 cggggcggcg gcctgtgccc tcgatgctcg cctcgagcac gtcggcatgc gaggccggaa
    27781 aatgacaaag ggattatagc tgcggtgcgt gggcgccgcc aggcggaagt tcaagcgccg
    27841 gcggcggtgc ccgcaggcat gcgctgcggg cggaactggc gggtgtcgcg gtagtagccg
    27901 gcggactcgc tggccggcgt cacgccgggg atgcccagca gtggcagcgg ggcgaggctg
    27961 cgcgggctga agtcgccgat cgtggcggcg aggcgggcag cgagccaggc atcggtctcg
    28021 gcgcgcgccg cggccgcggg cagtgcgagc caggccggat cgaccacgcg gtggatggcc
    28081 ttggcgcaca ggccggggaa gggcgcgcgc agctggtccc aggtgccgtg gccgaagacg
    28141 aggaagcggg tgctgtgctg cagggcctcg cgccggttcc agaacacctc ttcccacgcg
    28201 tgcgcggcga gcagggcggg gacctgcggg tcggcggaca ccacgacgat gccgcactcg
    28261 tcgaactggg tcagcgcgtc gcgccgcggc ccgcgtccgg cgaggccggc ggcatcgcgt
    28321 gcgcgcagct cgtcgaggtg gaggcggttg cagcaggcct tggtgcgcgg ccaggcgcac
    28381 caggcgagcg cgttgaagaa gtcgtgccag tcggcctcgc gggtggggac ctcgccggtg
    28441 gcgaagatgc gcgcctcgta gccgggctca tcgtcgcccg gcgcggcgaa gcggatcggc
    28501 gcgccgccac cgctgcgcgc cgcgggcgcg gcctcggcga gcagggcgtc gagcgcggcg
    28561 cgcgagggca gcccgggtgt ggtgaagcgg gccagccacg gtgcgatcgg ctcgaacagc
    28621 cggcgctcgg cgaaggcggc ggggatggcg agggcgtcgt tcatctgtgg aattgtgccg
    28681 gctcgcgggc ggtgcagcca gggtgcgtgc cgcggtcggg gtttcgagcc tttggcagga
    28741 gcgaggcagg tcgcgattgc cgcgccattc gcaggagacg acgtgtcgcg tcgcgtgagc
    28801 gctcaaatcg cgacttgcgt cgcgcctgcg ccaaaccctt ggaagtcgcc cgggactccg
    28861 gatcatccgc ctcagcgcat ctcgaacagc tcctggtgga agcgctcggc gggcaggccg
    28921 cgggcggcga gggtgtcgcg cagcatcgcg ccgaagccgg ccgggccgca gaaccacacg
    28981 ccggcctgct tccagtcggg cacgctgcga cagatgccgt cggcgtcgag gcggccgtca
    29041 cgtggcgaga ccagcacgtg cagctgcacc cgcgcccgcg cggcgagttc gcgcaggcgg
    29101 gcgatgaagc cttcgtcggg ggcgctggtg ctgtagaaga ggtcgatcgc cgccgcgcgc
    29161 ccacccggcc tgtcttcctg cgcacgcgcc tgcatgcggg cgacgaaggg ggtgatgccg
    29221 atgccgccgg cgacccagat ctgccgagct tgggcgccgc cgaagtcgaa gcggccgtag
    29281 gggccttcga cgcgcaccgg gtcacccagc ttcagcgtcg ccggcaggct gcgggtgtag
    29341 tcgccgagcg gcttgatcat gaaatccatg cggccgtcgc cgccccaggc ggaggagatc
    29401 gtgaaggggt gcggaccctc gcgctcgtcg aaggtgacga aggcgaactg tccggcctgg
    29461 tgtcccggcc aaggcgtgtc gaggcgcagg ccgacctcga gcacctggtt ggcggggtgc
    29521 cgcaccacgc gctcgatgcg gccgtgcgca cggcgcccgc ggccgatgcg gccggcgagc
    29581 gagcgcagcg cggcccagct gccggcggcc atcagcatgc ccatgaccat gccgagcggc
    29641 tgcgtccagt aggcgaaggg cagcagcacc acgccatgca cgaccagtgc gaggtacacc
    29701 gccgccatca cgcggtgggt ctgtacgaag cggcggtagg ggaagcgctt gatcagcgcg
    29761 atcgcggcga gcacggcgaa gaggtagaag gcccactcgc cgatggtctc ggcgaggccg
    29821 cgctgctcct ggaagaagcg gaagagctcg acggtctgct cggggcgcgg gccgcgcgcg
    29881 ggtttctgca gccagcccca gccgaccgcc cacttggtgc ccttggccca cagccagtgc
    29941 accacgccga ccgacaggcc ggcgatgccc agccacttgt gcagccggta ggccttgtcc
    30001 atgccgccga gcagcggctc gagcgcggcc gggcgcaggg cgaggatcat cgcgatgctc
    30061 atcgcgccca tggcgagcac gccggtgtag ttgaccgcga gcgtgcgcag cgcgaagtag
    30121 gtgtcgccct ggacgagcag cggatcggcc caccaccaca gcaggctgag ggtggcgagg
    30181 acgaggagga gtgcgagttt gatgttcttc atgatggctc ccttcgagcg gaatgcggcc
    30241 gccatgaggc agcggatccc atgcgggaaa ttgtgcgcca cagcatgtgt ccgccttgta
    30301 ccggcttgta gcccgcgcgg gcgcctgcgg ctggtcagcg tggcggcgcg cctatagact
    30361 cgcgggcgaa cctcgaccgg ccgccgtttg cggccaccgc acggcacccc gaacatgttc
    30421 cagcactccg ccccctcccc cgcctggctc cagcacgaca tggagcgggt cgacgcgatc
    30481 atccgcgcgc acatgaacaa cgacgtggcg ctgatccgcc aggtcgtcga gttcatcctg
    30541 cacggtgccg agcgccggcg gccggcgctg gttctggcga gcgcgcgcgc cctcggtcac
    30601 ggcggcgagc gcccgcacgc gctggcggcg gcggtggagc tgatccacat ctcgctcgcg
    30661 ctgcacgacg acgtcaccga ggcgagcggc ggcgcggccg acggcgagcg cgagggagcg
    30721 ctgttcggca acgccggcaa catcctgctc ggcgacctgc tctacaccag cgccttccgc
    30781 atgatcgtgg acctcggcga catggaggtg atgcgggtgc tgtccgatgc gaccaacgtg
    30841 atcgccgagg gtgaggtggc gtaccgcgag ttgctcgccg cgccggcgtc ccgtacgccg
    30901 gaggcctggc tggacatcgc gcgccggcgg cgcgcaaagc tcttcgaggc gggcgcggag
    30961 tgcatggcga tcgtgcacgg cgccgatccg gccgcgcgtt cggcgctggc gcgcttcggc
    31021 ctgcatgtgg gcagcgcgca tgcgctgcgc agcgaggcgg aggacgcggc gcgcttcggc
    31081 atcgccgacg cgcaggcgcg tgcgcaggcg gagatcgagg cggcgcgggc ggtcgccgac
    31141 gggctggcgc tggcgaacgc cgccgcgttg ggcgtggtgg tcgagggttg agcgcgggat
    31201 ccctgcccaa ggcctcgcgt tgacatgggt tcgatcgcct gcggatggct tgtcgcgggc
    31261 tgtctgcggg agcgacgcaa gtcgcgatta cggcggtcgc gattcgacga cgcccgtcgc
    31321 caccacgtcg tccggatcgc gacttgcgtc gctcctacac ggaccagcgc ggtctctcga
    31381 gctttcgtag gcgcgacgta agtcgcgatt acggcggtcg cgattcgacg acgcccgtcg
    31441 ccaccacgtc gtccggatcg cgacttgcgt cgctcctaca cggaccagcg cggtctctcg
    31501 agcgtttgta ggagcgacgc aagtcgcgat tgtggcgggt aggctcggac tccggctgtc
    31561 gtcaccgtgc catcaggccg cccggcactc gttggccagc ggcttgtgcg tgcggccgtc
    31621 gagcagcagg cccttccacg gcaggtcgat ccagaccagg ccgctggcgc cgtcctcgaa
    31681 gcgcggcagg ccggaatacg acaggtcgcg ctcgagctgg tactggctgc cgttccagcc
    31741 gagctggata cggttgttca ccgcgttggc gagctcgcgg cggatctgca ggcgttcgcc
    31801 gcgctcgcag cggtattcgc cgctggggag ggcgaactcg aactggccgg tctgctgggt
    31861 cacgcccggg gcgacctcgg tcttctgcgg gagcaggccg catgcgcccg tcgcgagggc
    31921 ggcgacgaag aggagtgcgc ccgaggccgc acgcacgggc ttcttcatgc ttcgcatcga
    31981 accggctcca aaggaaagac tccgaccacg aagccgggga aggggcggcg cccggtggcc
    32041 gtagccatca cttcacctcc agcaggctgt cgtcccactt gtcgtgcttg tgcaggccga
    32101 gcaggttggc caccgtggcg gcgatgttcg acaggccggc ggtctcgagc ggcttcaggc
    32161 cgagtttgcc gccgctgacg ttgtcgtaga ggatcagcgg caccgggttg agcgtgtgcg
    32221 cggtcttggc cttgtagctg ccgtccttgt tctgcgccgg ctgcctggtc ttcttgtcga
    32281 gctcgaacat ctcgtcggca ttgccgtggt cggcggtgat cagtgccacc ccgccggcgg
    32341 cgtcgatcgc cggcagcagg cgcgcgagcg ccaggtccac cgcctcgatc gccatggtcg
    32401 cggcgcggaa gttgccggtg tggccgacca tgtccccgtt ggcgtagttg cagcgcagca
    32461 ccttgaaccg gccggactgc agcgcggcga tcatcgcgtc ggtgatctcg gccgccttca
    32521 tccacgggcg ctgctcgaag ggcacgacgt cgctggggac ttcctgccag gtttcgccgt
    32581 cgaacttgtt cgagcggttg ccgttccaga agtaggtgac gtggccgaac ttctgcgtct
    32641 cggagcaggc gaactgggcg aggccggact tgctgaacca ctcgctggag gtgtccttga
    32701 tcgccggcgg ggcaaccagg aaacgcttgg gcagttgcag gtcgccgtcg tactgcagca
    32761 tgccggcgta ggtcgtgcgc ggatggcgca cgcggtcgaa ttcggtgaag tgctcttcga
    32821 cgaaggcgcg cgtgatctcg atcgcgcggt cgccgcggaa gttgtagaac acgaccgcat
    32881 cgccgtcctc gatggtgccg atcggcctgc catcctcggc aacgatgaac tcgggcaggt
    32941 cctggtcgat ggtgccgggg tgcttctcgc gcaggccgtg caccgcgtcg gtggcgctgg
    33001 cgaactgcgg gccttcgccc agcacgtggg tgcgccagcc gcgtgccacc atcggccagt
    33061 tggcgtcgta gcggtccatg gtgatggtct ggcggccgcc gccggaggcg atgcgggcgt
    33121 cgaagccggc agtgctgatg tccttcagga aggcctcgaa gggcacgacg tagtcgagcg
    33181 cgctggtctc gggcacgtcg cggccgtcga gcagggcgtg gatgcgtacc gtgggcacgc
    33241 cctcctcctt cgcgcgcagc accatcgcct tgagatggtc gatgtggctg tgcacgttgc
    33301 cgtcggagaa caggccgatg aagtggagca cccctgcttt tccgttggct cccgtcttgg
    33361 cgccggcgac gatctgctgc caggcctcgc cctgccagat cgcgccttcg gcgatcgcgt
    33421 tggcgaccag tgccgcgccc tgcgcataca cctggccggc gccgatggcg ttgtggccga
    33481 cctcggagtt gcccatgtcg tcgtccgacg gcatgcccac cgcggtgccg tgggcgcgca
    33541 gcacgatgtt ggggtactgg gcgaacaggc ggtcgagcgt gggcttgcgc gcggcgtcga
    33601 tggcgctgcc gacctcgtgc ttgggaatgc cgtagccgtc catcacgatg acgacgacag
    33661 ggccctggac gccggc
//
LOCUS       DAOQBS010000019        33324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_724_length_33324_cov_13.760498, whole genome shotgun sequence.
ACCESSION   DAOQBS010000019 DAOQBS010000000
VERSION     DAOQBS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 33324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33324
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_724_length_33324_cov_13.760498"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PKN25_03980"
     CDS             <1..476
                     /locus_tag="PKN25_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV90087.1"
                     /translation="LYAIEKDARELNDAQRLALRQSRSRAVLDELRRWLDQLLPTVPP
                     TSVLGGALGYLHRQWPRLTRYLERGDLPIDNNPAENAIRPFVVGRKAWLFSDTQAGAR
                     ASALLYSLVETAKANNVEPYLWLRHVLRALPTATTVEHFEALLPWNLNAEQLITA"
     gene            515..868
                     /locus_tag="PKN25_03985"
     CDS             515..868
                     /locus_tag="PKN25_03985"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV90088.1"
                     /translation="MISPAGTQNFSSYLSVDTGVMGYWSAALDSDFFTLNPNNQRCGD
                     PGVSCVRANDMRTAGDFTRLGAENWDVANTDIIGLGKTGTSTAFVVPEPTSLILLGLG
                     VALIGVSRTERRARR"
     gene            1041..1793
                     /locus_tag="PKN25_03990"
     CDS             1041..1793
                     /locus_tag="PKN25_03990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004319055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV90089.1"
                     /translation="MVRKLEGKVAVVTGASSGIGKSIVESYAQEGARVVAFARNLDAL
                     KALETAWPGQVRAVAGDVTRAEDLQRLVDEAVRVHGGVDVVVPNAGIARVVSFADSTA
                     EAFHTQFSVNLFGAAETARLFVPHIRKGGSIQFITTFLTQVGFPGLAIYSASKAALKS
                     LSQTLAAELAPLGIRVNSIAPGPIGTPLWGTVGLPPEILGAVAEKITSRLMPGAFGQP
                     EDIAELSVFLASDSARNIYGQEIVVDGGYTVG"
     gene            complement(1814..2545)
                     /locus_tag="PKN25_03995"
     CDS             complement(1814..2545)
                     /locus_tag="PKN25_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90090.1"
                     /translation="MADAVLKLEGLRKAYAGADTEYEVLHGIDLRLGRGEFAALIGPS
                     GSGKSTLLNLIGLLDRPSSGRLVIDGEETATLDDTALTRLRGERIGFVFQHHHLIPAF
                     TAEENVAMPLLIARGRPDAAMFARAGELLARVGLARQRRQLANRLSGGQQQRVAIARA
                     LAMSPALVLADEPTGNLDTHSADEVFALLREVNRQLGTSFLIVTHDPRLARRCDRIIE
                     LVDGVVVSDRPNNAAPPDSGPVDGS"
     gene            complement(2538..3746)
                     /locus_tag="PKN25_04000"
     CDS             complement(2538..3746)
                     /locus_tag="PKN25_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV90091.1"
                     /translation="MPFEWIAALRFLQEGRMQSLLIIVGVGVGVAVIVFMSALLSGLQ
                     ANLVRRTLSSQAHIVLLPAEEVARPQASSGHDAIRLQKQAQRLRSIDQWQLLRDRLEA
                     WPEIAAVSPAASGPAFAVRGDASKAVTLLGIEPARYQQVIDLGGRITAGELRVGAGEA
                     VIGIELAKDLGADVGDKLRIRGAQGEAETLTVTGLFDLGNKGVNARNVYVGLRTGQTL
                     LDLVGGVSNIDLALHDLDLAEDVAQRIAAESGLIADSWIRTNAQFVTALTSQRVSSNV
                     IRFFIALSVSFGIASVLVVSVVQRSKEIGILRAMGATQAQMRRIFLLQGGIVGFLGSF
                     LGSALAWAFLMLWRMLARNPDGTPLFDIGVEPALVAIAAGGASVVGILAALLPARRAA
                     GLDPVVAIRG"
     gene            complement(3755..4915)
                     /locus_tag="PKN25_04005"
     CDS             complement(3755..4915)
                     /locus_tag="PKN25_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV90092.1"
                     /translation="MLAISALAFATFHHETVNVALVEEGPLRQAIVASGRVRTPERIV
                     VSAQIAGRVQAVEAREGDTLAPGQALLRLDEREWGAAVAQAGAAVVQGEARLRQLREL
                     GLPVAEQALRQAEATALQAQRHHRRVQELVARGFYSQAQLDEARRALDVADSQVRSAR
                     LQRASQQPGGSEIELARSALEQARAALAAAETRLSYATLRAPVAGTVLTRNVEAGDTV
                     QPGTPLMTIAPAGATELTVQIDEKNLALLHPGQAALASADAYPGERFAAVLAYIAPSV
                     DAQRGSVEVRLRVPEPPPYLRHEMTVSLDIEAARRERTLAVPAAAVRESGGAQPWVLV
                     VRDGRTARQPVRLGIRGAGRVEVLEGLAAGEAVVPAPADVPAGRAVRTRVQP"
     gene            complement(5309..>6473)
                     /locus_tag="PKN25_04010"
     CDS             complement(5309..>6473)
                     /locus_tag="PKN25_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90093.1"
                     /translation="AEAAASRLPERIRNSAVSASIGIGAVATVLVSSWQAIDGAIGAL
                     AEERKTERAAILKITEDWAALPGRDRCSTTLMSEEHPKLTALAGSFLHHADALCARRE
                     ADWLAESSLVQEIFDEVAERCGLVETRMRSRACAALLVANTRPDAGGAEWPQICFAGR
                     PAAPAKTRLPTYWAYRYGFEAQRGKSAHPADSFAEAREFARAEHNAALACPTVALAGE
                     QPTPPTAQSPAAKPPPPPTAPPPRPAPVPPVAPAPAPAPAPAPTAPAAVEANSKICAG
                     TTIYLQIFGPTQRDTVRDYRDAWRKLGASVPPIEDVEATARAAQRTPPRQVALTTVRH
                     HDPASKRCAEELGKAVGFGNWKVEPLAASLKPTRGVIEVWIGREIPARQGEAK"
     assembly_gap    6474..6573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6574..9192)
                     /locus_tag="PKN25_04015"
     CDS             complement(<6574..9192)
                     /locus_tag="PKN25_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNV90094.1"
                     /translation="MSEKIDQLVGERRAALGRKDGVADKWGLALSGGGIRSATFCFGL
                     LGVLARNRLLERFDLLSTVSGGGYIGGMLGRLLHGARSADDTRAIFAAFGSQEPRWFR
                     WWLRANGRYLVPRGPADKTFALAVFLRNLLAIHLELGALALALGVVLAAVDVGVWAAI
                     AHWVTDGWVTGERIEALRWLPPWLPTLWPVAIPVLAVLGGLATAAYWVVPWVASAGRP
                     GWRPGFVAVQCVILTIVLGLLVHYGDAIIGPQWQPGHAIRMALTWGAIGLAVAWLIAI
                     PWARFLLRHLFDETERDALRLREEAVRRWLADWQSLCMKAIAVVLLLGLVDRVAWFLA
                     FEFKAQANTALALAVAAAVLRAAMPALGGGPQGGISGKLLLSLGQLSGYILSFLLCAW
                     WVSLVHAAALGPMFATEGTVDFGTPWLRLLAIGTAAGGYVLATGRNFEFLNLSSLHTF
                     YRARLVRSYLGAANPARFGQQDTLGPTAVVSERGAAHLAHKSVFDPDPDDDLALEAYA
                     PHRQGGPVHLIGVCINQTHDPRGGLFNRDRRGLPLTVGPEGWVRVGQEPWFQVTGAGA
                     LGLGSWVAISGAAVSPGLGSQTRGGISALLAFAGIRLGYWWTRAARENLQRPKRRLAA
                     KSRGLLSEVFCNFKGSSGPDWFLSDGGHFENTAAYALLAERCRMIVLADCGADPDYRF
                     GDLENLVRKARIDLGAEIEFLRPRPRAAGENDRPPGVELFGSLNDLASRKSNACLALA
                     RVRYVGATEPGWLVLVKPNISAGLPVDLINFAASNPAFPQQTTADQFFDEAQWESYYQ
                     LGFNLGDVLDSDLLEALRSRHATLFESDTGALTAATPRASVTPATVPAGAGAAAAAAA
                     EAAASRLPERIRNSAVS"
     gene            complement(9370..12219)
                     /gene="fdhF"
                     /locus_tag="PKN25_04020"
     CDS             complement(9370..12219)
                     /gene="fdhF"
                     /locus_tag="PKN25_04020"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNV90095.1"
                     /translation="MSRHDREDRSPTPQPPGLATAPDRTAAHDALDQRADAFEILLDG
                     VTAQAFPGETLWQVALRAGERIPHLCFKDAPGYRADGNCRACMVEIEGERVLAASCIR
                     AAQPGMVVRSASSTRARQAREGVLELLLEQQPARTHSPDRSSHFWELVEHMRLPAADR
                     GPAAQQPDRTHPAIHVNLDACITCGLCVRACREVQVNEVIGLAHRGARAKIVFDFDDA
                     LGDSSCVACGECVQACPTGALMPATLVDAQGRGDSACAERKVDSVCPYCGVGCQLTYH
                     VRDERILFVEGRNGPANENRLCVKGRFGFDYPNHRGRLTTPLIRRAGVPKGVDPAFDP
                     ANPLSHFRPASWDEALDFAAAGLRRLLDAHGPGVLAGFGSAKCSNEEAWLFQKLVRTG
                     FGSNNVDHCTRLCHASSVAALMECIGSGAVTASFMQAAHADVVILTGCNPTVNHPVAA
                     TYFKQAAKRGTRLIVLDPRGLVLGHHAHRMVRFTPGSDVALFNAMLNVIVGEGLCDRD
                     FIAARTEGFEALAAHVAPLTPEAMAPLCGVAPDEIRAIARLYATAERAMIFWGMGISQ
                     HVHGTDNARCLIALALATGHVGRPGTGLHPLRGQNNVQGASDAGLIPMVLPDYRPVGD
                     AQYRAAFEELWATPLPAEPGLTVVETMDAIAAGRVRGMYILGENPAMSDPDLHHTRAA
                     LARLEHLVVQDLFVTETAQFADVILPASAWPEKDGTVTNTNRQIQLGRAAVPLPGEAR
                     PDWWILQQLARRLGLDWQYAHPREVFAEMKRAMRSLDHIDWARLEREGAVTTPCPAED
                     APGKDVVFDDRFPTASGRARFRPTVPLPPDEPVDAAWPTVLITGRQLEHWHTGAMTRR
                     SAVLDALEPAAVATLAPAELTRLGLAPGAALSIETRRGRITLAARADPLMPAGMVFVP
                     FCYVEAAANLLTNPALDPYGKIPEFKYAACRLGPG"
     gene            complement(12216..13967)
                     /locus_tag="PKN25_04025"
     CDS             complement(12216..13967)
                     /locus_tag="PKN25_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNV90096.1"
                     /translation="MPSPSPSPSASQAAGRHTRPGLRGRQTDPAALAEIEALLGAARR
                     ERDELIEHLHALQDRFGHLSLRHLRALADWMRMPMAEVYETATFYAHFDVVREDEPVP
                     PALTVRVCDSLPCQLAGAQALRAALDAALDPARIRVLRAPCMGRCDQAPVAQLGRRHL
                     SRATPAAVLAALARGALDPEPIAWQRLADYRAAGGYTLLQRLRSGETGVAALEARLAE
                     AGLRGLGGAGFPTARKWQAVRAGAAPRYLVVNADEGEPGTFKDRHYLETAPHRVLEGA
                     LASALAVGAAAIYVYLRDEYPGLHAVLREAIAELEAAGLVAPGFIVLRRGAGAYICGE
                     ESALIESLEGKPGKPRHRPPFVAEAGLFGRPTLVNNVETLYWIPLLAAGAAFAGEGRR
                     GRGGLRSFSVSGRVNKPGVHLAPAGITLRELVDEHCGGLQPGHRLLAYLPGGASGGIL
                     PAALADLPLDFDTLQPHGCFIGSAAIIVLSDQDDLRAVADNLLAFFADESCGQCTPCR
                     LGTEKLLALLRTDDWDVARLQALAQTLRDASICGLGQAAPNPVSSLLRFFPAELARAG
                     VRLHAGPPARDAGELQP"
     gene            complement(14001..14495)
                     /locus_tag="PKN25_04030"
     CDS             complement(14001..14495)
                     /locus_tag="PKN25_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HNV90097.1"
                     /translation="MPRRLLRALRLAAICCALALAPATSSSARAEGIAVEHFPGVPFA
                     DLREALADAIADEGLSAPVVSRFGAMLERTAPDLGHDPALYGDAEVLSFCSAGVAARL
                     VAEAREHIALCPLSIAVYSLPHTPRDATLAWRPPTLDSPGGDAVRALMARIVTGAARL
                     VGRR"
     gene            14651..15124
                     /locus_tag="PKN25_04035"
     CDS             14651..15124
                     /locus_tag="PKN25_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HNV90098.1"
                     /translation="MRPLVARLLTAWIIAQAPGAAHALEPEELDALVARPSIGLYKAY
                     AEFKMARYGSAREIWSALAQAGVAEAWFHLGILAEDGLGEPRDAAAALERYRRGGEAG
                     SSKAQYRLGLLHLEGRLVPADPVQARYWLAVAAANGDEEAARRLATLPASIPAAR"
     gene            complement(15216..16244)
                     /locus_tag="PKN25_04040"
     CDS             complement(15216..16244)
                     /locus_tag="PKN25_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HNV90099.1"
                     /translation="MSRPASCAPALQRRPAARALRTLLAGLLVALCALLLTALPAHAE
                     DFAGDFNYRLQPRELAPGAWVVVGLKEDFSFANGGNIVNTGFIVGREGVIVIDTGSSK
                     RYGEQMLAAIRRVTPLPIVLTVNTHHHPDHFLGNQAFPRATLAALPETIAALRSEGEG
                     FNANMYRLNGDWMRDTEVVVPERALAAGRQRIGGRDVELVALGGHTVADLVVIDHDSG
                     TVYAADLLFNGRAPTTPHADIARWLAALDTLEGLPARRWVPGHGEPATDLAPLRQTRD
                     YLGWLAQAIRAGAESGQDMTELFQTPIPQRFAGLALVEAEFRRSVVHLFPAAEQAVLE
                     AARNKASR"
     gene            complement(16241..17056)
                     /locus_tag="PKN25_04045"
     CDS             complement(16241..17056)
                     /locus_tag="PKN25_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HNV90100.1"
                     /translation="MRPSARPALAWIAAALTALASPAALAAAPRAGAPSPSAAHTDPL
                     DSPRWEDMRKSFFADVTVVFDERVKVSGPAVAEDSLNVPVNVDASALPGVEELIVFAD
                     FNPIVKALRFEPGAAQPRLGFRIKLQQSSPVRAAARTADGVWHVGGAWITATGGGCTL
                     PSVGSGSPEWQERLNEVNGKLWPRVDGGERLRMRIIHPMDTGLAAGIPAFHIEDIVIA
                     DAEGRTLSRIQPLEPVSENPSFTLDLRAAAPGARLHVAGRDNNGNRIDAWVTR"
     gene            17389..19197
                     /locus_tag="PKN25_04050"
     CDS             17389..19197
                     /locus_tag="PKN25_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HNV90101.1"
                     /translation="MMKNDRRARTLPAMLTALTVALAAAGMGATAQAASVSDEDILKD
                     AETTHQVVTNGLGTKGQRYSPLAKVNVDTVKDLVPVWAFSFGGEKQRGQQSQPLIHDG
                     KMFVTASYSRIYALDAKTGQKLWKYEHRLPEGIMPCCDVINRGAALYGDLVIFGTLDA
                     QLVALNRDTGKVVWREKIDDYKAGYSMTAAPQIVKGMVITGVSGGEFGVVGRVEARDA
                     KTGKKVWVRPVVEGHMGYKYDKDGKEIENGISGTTNATWPGDLWKTGGAATWQTAYYD
                     PDVNLIFMGTGNPAPWNSWLRPGDNLYSSSTVAVDPDTGKIVWHYQNTPHDGWDFDGV
                     NEFISFEYKDPKTGKLVKAGAKADRNGFFFVNDRTNGKLLGAYPFLTRIDWAKGYDLE
                     TGRPITNDDKRPPNPFAEGAAGEGGKGKPVFAAPSFLGGKNQMPMGYSPDTGLFYVPA
                     NEWGMDIWNEPVSYKRGAAYLGAGFTIKPLYEDYIGAMRAIDPVSGKIVWEVKNNAPL
                     WGGVLSTAGNLVFYGTPEGYLKAVNAKTGEEAWKFQTGSGVIAPPVTWEADGEQYVAV
                     VSGWGGAVPLWGGDVAKRVNFLEQGGTVWVFKLHKS"
     gene            19364..20221
                     /locus_tag="PKN25_04055"
     CDS             19364..20221
                     /locus_tag="PKN25_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNV90102.1"
                     /translation="MTKLQLSSFATAVVLAVGVGTAAHAAERATPREARTMFEQAVRY
                     MEENGPERAFAAFNDRQGGFVRKDLYVFAIDDKGVYQASGAAPEALVGLKVLDTTDAA
                     GNPLFREMIDATRVSQEATVRYVWLNRASNKVEPKVSYVRKVGQYVVGVGYSAPRASA
                     DDARAMLERAVAVARGEGGARAAARFNDRRGDFVKDDLYVFMVNMESGRFEAMGMNPA
                     LSDTDALGLRDAAGNPLIAEMIARLQQADEATVDYVWRNPVTNAVEHKRSYVRKVGKS
                     LVGVGHYLE"
     gene            complement(20386..21231)
                     /locus_tag="PKN25_04060"
     CDS             complement(20386..21231)
                     /locus_tag="PKN25_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNV90103.1"
                     /translation="MKTDRRRFLLAAGALAVLAASPARAAAAELELQQPGTLRVAVYA
                     NFAPWSTAGKGIDIALGRALAERLGLQPQVVEFPADENMNDDLRNMVWRGHYLGHKPG
                     DVMLHVPVDPRFAALNKQVKIFGPYHLETIALARLPERVPAPGGSADASFAVFEQERI
                     GAELDSHASDFLRHILGGRLQPNVAHFRNVGEAVAAMKRGEIAAVMGARSELETAIGG
                     DAAIALDTLSMPELRVASWPLGMAVKAEHSGLEQALSGAIAELQRSGELGRIFAAHGV
                     RQRTP"
     gene            complement(21221..21670)
                     /gene="pedF"
                     /locus_tag="PKN25_04065"
     CDS             complement(21221..21670)
                     /gene="pedF"
                     /locus_tag="PKN25_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HNV90104.1"
                     /translation="MNTRTIKENLRKRLAITATGIALAAASALAHAHGDVTPQAVDTS
                     SLKQLGGTWVTVNPYRGNKEAIRIGDSAFNQNCARCHGLGAVSGGIAPDLRLLDVADD
                     GTDEFFIGKVRNGVTRNGAVYMPPFEGMLTQEAMWAIRAWLETVHEN"
     gene            21958..24057
                     /locus_tag="PKN25_04070"
     CDS             21958..24057
                     /locus_tag="PKN25_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNV90105.1"
                     /translation="MKVMQQTAMAPAVREDAVRLARGQFFGEGRAPEAGVSPLIVDSW
                     LRCRGRGMAADDRAEAEHAGRLQLAEAREGNRSLLDYASGVMEHVFEQIRASGSLVIL
                     SDPQGMILHSLGDAEFVDRASRVALQPGACWSEAARGTNAIGTAIVTEAPTVVLGGEH
                     YLDRNGFLSCTAAPIRDPRGALAGVLDVSCEQRMHQRHSLGLVRLSVQLIEKRLFEAE
                     FANHILVAFHERPEYVGSLQEGLVAVTPEGHLAGANSIAREWIGRRVECGGGASFAEL
                     FRQGLGKVVDRAMNSSDALIRLGLRDGSEIHVQLRSLRPRFTAFGSAQAGEGMRTFAA
                     ESSPAAVGSAPPVSAPAPVSAPHGPITLHDLATGDAGIGRAVDRARRILGKDIPLLIQ
                     GESGVGKELFAQAFHNSGPRADKTFVALNCAAVPETLIESELFGYVGGAFTGARKEGA
                     LGKIQQANGGTLFLDEIGDMPLGMQARLLRVLQERCVTPVGSVKSIPVDISLVCATHR
                     VLRDAVARGEFREDLYYRVNSLTVTLPALRERSDIRCIVERILAAECREAGLQGVWIG
                     DEVMRFVERHGWPGNVRQLQNVIRVAVALLDEGEREIRPEHLPEDLFLADPVDGGKTP
                     PAAHAGPLVAAASAALPLAADAPPISAAKRLDQLEIELIERVMREVGGNLSAAARTLG
                     VSRNRLYRKLGRAGGVS"
     gene            24188..24667
                     /locus_tag="PKN25_04075"
     CDS             24188..24667
                     /locus_tag="PKN25_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV90106.1"
                     /translation="MWKALHIDPAKCTGCLQCEMACSYEHTGVINPSKSRIKVFNFEH
                     EGRKVPYTCTQCTEAWCLNSCPVDAIRIDLSTGAKMVFEDTCVGCKVCTIACPFGTIN
                     YNQDSGKVQKCDLCEGNPACASACPTGAITYVDADWTGIDKMRAWAAKANTPASAAA"
     gene            24680..26527
                     /locus_tag="PKN25_04080"
     CDS             24680..26527
                     /locus_tag="PKN25_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNV90107.1"
                     /translation="MGWNRKVLRVNLTAGTCTEEALNMQWADEYLGSRGLATKYLVSE
                     IDPRVDPLSPDNKMIMATGPLTGTMASTGGRYTVVTKGPLTGAIACSNSGGFFGAEMK
                     FAGWDMVIFEGKSAKPVWLYIENDKAELRDASGLWGRSCWETEEKIKEQLQDPLVRIS
                     SIGVSGENQVLYSCIVNDLHRAAGRSGVGAVMGSKNLKAVAIRGTKGVSGIKDFGAFL
                     KATNDAKKVLADNAVTGQGLPKYGTQVLMNVINEIGALPTRNHRDVQFEDASKISAEA
                     MHEKRASDGKTHLVTNAACFGCTIACGRISEIDKTHFTVKNSPKYWGASGGLEYEAAW
                     ALGAANGVGDLEALQYANLICNEQGMDPISFGATVGAAMELYELGILDDAKIGVAAPF
                     GSAEALAKMVEMTALGEGFGKELGLGSKRLCEKYGRPELSMSVKGQEFPAYDSRGIQG
                     MGLAYATSNRGACHLRGYTVASEVLGIPVKTDPLTTDGKPELVKAFQDATAVFDSAGI
                     CVFTSFAWTLADVQPQVAAACEGDWSMEKLNEVGERIWNMERMFNNAAGFTAKDDNLP
                     PRLTQEAAKTGPAKGLVNGLAKMLPEYYQVRGWTPGGEPTAETRARLGL"
     gene            26760..28049
                     /locus_tag="PKN25_04085"
     CDS             26760..28049
                     /locus_tag="PKN25_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV90108.1"
                     /translation="MKHLILGNGPAGVVAAETLRKVAPADDILMVGHEDAPPYSRMAI
                     PYLLEGNIDESGTWLRKDPGHFERLRIRQLRGRAVALDADKRKVLFDDGHFEHYDRLL
                     IATGSHPVRPPIPGVDLPSVHTCWTLADARAIAAHARPGARVLQLGAGFIGCIIMEAL
                     AKCGVQLTVVEMGDRMVPRMMTPKAGGMIKRWVEEKGIRVLTSAGVQRIDRGSEAGSD
                     APLVVSLSTGDRLDCDLVIVAAGVAPNVGFLDETPVHVAKGVLVDDHMETSVPGIFAA
                     GDVAEAPDLFTGAHLVSAIQPNAADQARIAATNMARDRVHESRLNGVLAINVLDTLGL
                     ISSSFGQWWGEKPEAGGSGVEMVDEARYRYLSLQFKDDVLVGATSIGLTQHVGALRGL
                     IHGQVKLGDWKERLLANPLAFVDAYVARSQQPMALAR"
     gene            28072..28329
                     /locus_tag="PKN25_04090"
     CDS             28072..28329
                     /locus_tag="PKN25_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNV90109.1"
                     /translation="MTIRIAFKLFASLSDYLPDGHRGNRLELEVDEGTTVAEMIRRYN
                     VPERSAHLVLVNGHFVPPSERARKRLADGDELAIWPPIAGG"
     gene            28334..28642
                     /locus_tag="PKN25_04095"
     CDS             28334..28642
                     /locus_tag="PKN25_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90110.1"
                     /translation="MAFINGPVPESFERRVTATAREFERDLRQAWPGVEGGAASLRFR
                     LQDGAQRLVIDIEPAGVRRLGLFELPQLIVRYGFGPGEEAARRALLARLDRAMQKGGG
                     "
     gene            28676..28963
                     /locus_tag="PKN25_04100"
     CDS             28676..28963
                     /locus_tag="PKN25_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNV90111.1"
                     /translation="MSEAYVVGHITVKNAEMWAEYRSKVPQTLAPWGAELVFRGRQVA
                     ALAGESPHPDIVVIRFPSVAAVNDWFSSPAYQALIPLREQAADVLLLSYES"
     gene            29077..29781
                     /locus_tag="PKN25_04105"
     CDS             29077..29781
                     /locus_tag="PKN25_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV90112.1"
                     /translation="MSAFAATFSDALSLLAHFDHTVLEIVALSLQVSGAAVVLGTLIG
                     LPLGACIAVGRFPGKTTLSVLINGMMGLPSVVVGVVVYLILSRSGPFGALGMLYSPAA
                     MVVAQTLLVVPLMAAITRQVVEDVWARYAEELQVMRFSWWDSVTTLLHDCRHSLVVAV
                     LAGLGRAMSEVGAVMIVGGNIDGATRVMTTAIALETSKGDLALAIALGMVLIVIILAL
                     NALAYVLRQWAMRRFG"
     gene            29790..30524
                     /locus_tag="PKN25_04110"
     CDS             29790..30524
                     /locus_tag="PKN25_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNV90113.1"
                     /translation="MPIFPLSIHDLRFAPNGREVLAGVDLELSEEGITLVLGPNGAGK
                     SVLLRTICGLIEPSSGSMAWGGAGGTRPQQGVMMVFQRPMMLRMAVLDNVVLGLKPLG
                     VAGAERRRRGLEMLERVGLADRAGDSARQLSGGEQQRLALARAWLTRPRLLLLDEPTA
                     SLDPSASAAVERIIREIRTDGTRILMVTHNLGQATRLGDDIVFMAGGRVCEHTPVRAF
                     FARPRSEEARLFIQGELPWRMRFQGA"
     gene            30550..30801
                     /locus_tag="PKN25_04115"
     CDS             30550..30801
                     /locus_tag="PKN25_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90114.1"
                     /translation="MSPVPARLPLAALVPFVLAALAFVAALLCFAGAGALLHPALSDP
                     MAGLAFLVSAIALAGTGAFPLVFARLARRDAGPQPPPGA"
     gene            complement(30836..31324)
                     /gene="moaC"
                     /locus_tag="PKN25_04120"
     CDS             complement(30836..31324)
                     /gene="moaC"
                     /locus_tag="PKN25_04120"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNV90115.1"
                     /translation="MSTPESSPLTHFDADGQAHMVDVGAKAETRRVAVATGRIHMLPA
                     TFDMVKSGSAKKGDVLGIARIAAIQASKRTSELIPLCHPIPLTRVAVEFELDAAAHAI
                     RCTVTAETVGRTGVEMEALTAVNVGLLTIYDMCKAVDRGMRMEGIQLMEKLGGKSGHW
                     KA"
     gene            31448..32887
                     /locus_tag="PKN25_04125"
     CDS             31448..32887
                     /locus_tag="PKN25_04125"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNV90116.1"
                     /translation="MMRRPLALLLSLALALPAPAPVLAADLPDLGDVAASELSPAAER
                     KIGEQIIREIRWRDAAYLDDAEVEEYVNRLGQRLAAVSNNPGLDFDFFVVRDATLNAF
                     ALPGGFIGVHTGLILAAEGESEFASVLGHEIAHVTQRHIAQIVGKQSQSAMLMIASML
                     VAVLAARSNSDVSTAAIAAGQAGAIQSQLGYTRAFEREADRAGLETLDKAGLDVRGMP
                     GFFERLQRNTRVYENNAPAYLRTHPLTTERIADMENRVAAMRYRQVPDSADFRFARAK
                     LRANAGQPAEAVQELEDRLAREPKDEALAYGLARALMRAGRLDEAEARLQPLRAKAAG
                     LAWVEGLAAEIRLARNDAGGAIRILEAAQKQFPSSRSVSYALADARILGGRADVAATE
                     LRRRIDNRSGDPRLWQLLSRAYAALGQRTEQHRTQAEVYYLRGSLPAAIEQLEIARRA
                     SDGDFYTLSAVDARLRELKQRLLEEKRER"
     gene            32994..33224
                     /locus_tag="PKN25_04130"
     CDS             32994..33224
                     /locus_tag="PKN25_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNV90117.1"
                     /translation="MRYDKEVDARGLNCPLPILRAKKMLSEMQSGQIVRVLATDPGSI
                     KDFQAFARQTGNELLGQGETSDKAYEFFLKRK"
BASE COUNT         4813 a        11382 c        12321 g         4708 t
ORIGIN      
        1 agctctacgc catcgagaag gacgcgcgcg aactgaacga cgcccagcgc ctggcactac
       61 gccagagccg aagtcgcgcc gtcctcgacg aactgcgccg ctggctcgac cagctgctcc
      121 ccaccgtgcc gcccacctcg gtgctgggtg gcgcgttggg ctacctgcat cggcagtggc
      181 cgcgattgac gcgctacctc gaacgcggcg acctgccgat cgacaacaat cccgccgaaa
      241 acgccattcg ccccttcgtc gttgggagga aagcgtggct cttctccgac acccaggccg
      301 gtgcgcgtgc cagcgcgctg ctctactcgc tggtcgagac cgccaaggcc aacaacgttg
      361 agccgtacct gtggctgcgt cacgtgctgc gcgccttgcc caccgccacc accgtcgaac
      421 acttcgaggc cctcctgcct tggaatctca acgctgagca gttgatcaca gcgtagcgag
      481 ccggggaagg atggggttcg tggagcgctt accgatgatc tctccggcag ggacgcagaa
      541 cttctcgagt tatctgagtg tcgataccgg tgtgatgggc tattggagcg ccgcgctcga
      601 ttcggatttc ttcacgctca accccaacaa ccaacgatgc ggcgacccgg gcgtctcttg
      661 cgtccgcgcc aacgatatgc gaacggcggg cgacttcacg aggctcggtg ccgaaaattg
      721 ggatgtcgcc aatacggata tcattggact gggcaagact ggaacctcga cggcgttcgt
      781 cgtgccggag ccgacatcgc tcatcctgct cggacttggc gtggcgctga tcggcgtgag
      841 cagaacggag cgccgggcaa ggagatgaaa ggattcggtg ggccaagggt gttcatcagc
      901 aggcgcgcat tcgaaatgcg cgcctgcttt cgtcttgtgg gcgaagagcg tgagcgcgac
      961 gcaaaggagg gcctgatgag ctgtctggag atggatgagc aaatggaaga cctgacccca
     1021 tcgtatttca ggagcgagac atggtgagaa agcttgaagg caaggtggcc gtcgtcacgg
     1081 gcgccagcag cgggatcggc aaatcgatcg tcgaaagcta tgcgcaggaa ggcgcccggg
     1141 tggtggcgtt cgcgcgcaat ctcgacgcct tgaaggcact cgagaccgct tggcccgggc
     1201 aggtccgggc cgtggcgggt gacgtgacca gggccgagga cctgcagcgc ctggtcgatg
     1261 aagccgtccg cgtccatggc ggtgtggatg tcgtcgtgcc caatgccggc atcgcgcgcg
     1321 tcgtgtcttt cgcggacagc accgccgaag ccttccatac gcagttctcg gtcaatctct
     1381 tcggtgcggc ggaaaccgcg cgtctcttcg ttccccacat ccgcaagggg ggctcgatcc
     1441 agttcatcac gaccttcctg acccaggtcg gctttcccgg gctggccatc tacagcgcca
     1501 gcaaggctgc gttgaagtcc ctgtcccaga ccctggcggc cgagctcgcc cccctgggca
     1561 tccgggtcaa ctcgatcgcc cccggcccga tcgggacgcc gctgtggggc acggtcgggc
     1621 tgccgcccga gatcctgggg gccgtcgcgg agaagatcac gtcccggctc atgcctggcg
     1681 cattcggtca gccggaggat atcgccgagc tctcggtgtt cctcgcgtcg gacagcgcca
     1741 ggaacatcta cggccaggaa atcgtcgtgg atggcggcta cacggtcgga tgagcttcgt
     1801 cgcgctcgcg gcctcatgag ccgtcgaccg ggccagagtc cggcggcgcg gcgttgttgg
     1861 gccggtcgga gacgacgacc ccgtccacga gttcgatgat gcggtcgcag cggcgggcga
     1921 ggcgggggtc gtgggtgacg atcaggaagc tcgtgccgag ttgccgattg acctcgcgca
     1981 gcagcgcgaa gacctcgtcc gccgagtgcg tgtcgaggtt gccggtgggt tcgtcggcga
     2041 gcaccagcgc gggcgacatc gccagcgcac gggcgatcgc gacgcgctgc tgctggccgc
     2101 cggagagccg gttggcgagc tggcgccgct gccgggccag gccgacgcgg gcgagcagct
     2161 cgccggcgcg cgcgaacatc gccgcgtcgg gccgcccgcg cgcgatcagc agcggcatgg
     2221 cgacgttctc ctcggcggtg aaggcgggga tcaggtggtg gtgctggaac acgaagccga
     2281 tgcgttcgcc gcgcaggcgg gtgagagcgg tgtcgtcgag cgtcgcggtc tcctcgccat
     2341 cgatgacgag gcggccgctg ctggggcggt cgagcaggcc gatcagattg agcagcgtgc
     2401 tcttgcccga gccggagggg ccgatcaggg cggcgaactc gccgcgcccg aggcgcaggt
     2461 cgatgccgtg caggacttcg tactccgtgt cggcgccggc gtaggccttg cgcaggcctt
     2521 cgagtttcag gaccgcgtca gccacggatc gccaccaccg ggtcgagccc tgccgcacgc
     2581 cgcgccggca gcagcgccgc gaggatgccg accacgctgg cgccgcccgc ggcgatcgcc
     2641 accagcgcgg gctcgacgcc gatgtcgaac agcggcgtgc cgtccgggtt gcgcgcgagc
     2701 atccgccaca gcatcaggaa ggcccacgcc agcgccgagc ccaggaagga tccgaggaaa
     2761 cccacgatgc cgccctgcag caggaagatg cgccgcatct gcgcctgcgt ggcgcccatc
     2821 gcgcgcagga tgccgatctc cttgctgcgc tggaccaccg acaccaccag cacgctggcg
     2881 atgccgaagg acaccgagag cgcgatgaag aagcggatca cgttgctcga cacccgctgc
     2941 gaggtgagcg cggtgacgaa ctgcgcgttg gtgcggatcc agctgtcggc gatcaggccg
     3001 gactcggcgg cgatgcgctg cgccacgtcc tccgcgaggt cgaggtcgtg gagggcgagg
     3061 tcgatgttgg agacgccgcc gaccaggtcg agcagggtct gaccggtgcg caggccgaca
     3121 tagacgttgc gtgcattgac gcccttgttg ccgagatcga acagcccggt cacggtcagc
     3181 gtctcggcct cgccctgcgc gccgcggatg cgcagcttgt cgccgacgtc ggcgccgagg
     3241 tccttggcca gctcgatgcc gatcaccgcc tcgccggcgc cgacgcgcag ctcgccggcg
     3301 gtgatgcgtc cgccgaggtc gatgacctgc tggtagcgcg cgggttcgat gccgagcagg
     3361 gtcaccgcct tgctggcgtc gccacgcacc gcgaacgccg ggccggaggc ggccggcgac
     3421 accgcggcga tctcgggcca ggcctcgagg cggtcgcgca gcagttgcca ctggtcgatc
     3481 gagcgcagcc gctgcgcctg cttctgcagg cggatcgcgt cgtgcccgct cgaggcctgc
     3541 gggcgcgcca cctcctcggc cggcagcagc acgatgtgcg cctgcgaact gagcgtgcgg
     3601 cgcaccaggt tggcctgcag accggacagc agcgccgaca tgaacacgat caccgccacg
     3661 ccgacgccga cgccgacgat gatcagcagc gactgcatgc gtccctcctg caggaagcgc
     3721 agcgcggcga tccattcgaa gggcatccac gacttcatgg ctgcacgcgc gtccgcaccg
     3781 cccgccccgc gggcacgtcg gccggcgccg gcaccacggc ctcgccggcg gcgaggccct
     3841 cgagcacctc caccctgccg gcgccgcgga tgcccagccg caccggctgg cgtgcggtac
     3901 ggccgtcgcg caccaccagc acccagggct gcgccccgcc ggactcgcgt accgccgcgg
     3961 cgggcacggc cagcgtgcgc tcgcgccgcg ccgcctcgat gtcgagcgac accgtcatct
     4021 cgtggcgcag gtagggcggc ggctcgggta cgcgcaggcg gacctccacc gagccgcgct
     4081 gggcgtccac cgagggggcg atgtaggcga gcacggcggc gaagcgctcg cccgggtagg
     4141 catcggccga ggccagcgcg gcctggccgg ggtgaagcag ggcgaggttc ttctcgtcga
     4201 tctgcacggt gagctcggtg gcgccggccg gggcgatcgt catcagcggc gtgcccggct
     4261 gcacggtgtc gccggcctcg acgttgcggg tcagcacggt gcccgcgacc ggggcgcgca
     4321 gggtcgcgta gctcagccgg gtctccgccg cggccagcgc cgcgcgcgcc tgttccaggg
     4381 cgctgcgcgc gagctcgatc tcgctgccgc caggctgctg gctggcgcgc tgcaggcggg
     4441 cgctgcggac ctggctgtcg gccacgtcga gcgcccggcg cgcctcgtcg agctgtgcct
     4501 ggctgtagaa gccgcgcgcg acgagctcct gcacgcgccg atggtgccgc tgcgcctgca
     4561 gcgccgtggc ctcggcctgg cgcagcgcct gttcggccac cggcaggccg agctcgcgca
     4621 gctggcgcag gcgcgcctcg ccctgcacca cggcggcgcc ggcctgggcg acggccgccc
     4681 cccactcgcg ctcgtccagg cgcagcagcg cctggcccgg cgccagcgtg tcgccctcgc
     4741 gcgcctccac cgcctgcacg cgcccggcga tctgcgccga taccacgatg cgctcgggcg
     4801 tgcgcacccg cccgctcgcc acgatcgcct ggcgcagcgg tccctcctcg acgagggcca
     4861 cgttcaccgt ctcgtggtgg aaggtggcga aggcgagcgc ggagatcgcc agcacgcccg
     4921 cgccgacgcc cgcgcgcgag ccgagcaggc gtcgcagggt ggaggggcgg ccggcgcgga
     4981 gaggatcgtc gggggtcttg ccgggtgtgt tcaggatgat cgtggctccg ctgtcgatgc
     5041 cgcgggctgg gtttgctgct gcgggtacga gggccgcgtc gtgccgcccg gccggcgccg
     5101 accggctcgg ggaggtccgg cggggttccg cggacggggc tcggaggtcg gggcaaggca
     5161 gggcggacgc ggcgggaacg cccacgagcc ggcgggaccc gcattgtcgc cgcccggcgc
     5221 ggtcgccgac aagcgccggg gctgagcttc gatcgcgtca ccgccgcgga ggcgcgcact
     5281 cgcccgcgat gcgcatgacg gcgtcgactt acttggcttc gccctgcctg gcggggatct
     5341 cgcggccgat ccagacctcg atcacgccgc gggtcggctt gagcgatgcc gcgagcggct
     5401 cgaccttcca gttcccgaaa ccgaccgcct tgcccagctc ttcggcgcag cgcttcgagg
     5461 cgggatcgtg atggcgcacg gtggtcagcg cgacctggcg cggcggcgtg cgctgcgccg
     5521 cgcgcgccgt ggcctcgacg tcttcgatcg gcggcaccga ggcgccgagc ttgcgccagg
     5581 cgtcccggta gtcgcgaacc gtgtcgcgtt gcgtagggcc gaagatctgc aggtagatgg
     5641 tcgtgccggc gcagatcttc gagttcgcct ccaccgcggc gggcgcagtg ggcgcgggcg
     5701 caggcgcggg cgcaggcgca ggcgcgacgg gcggcacggg cgccggtcgt gggggcggag
     5761 cggtgggtgg agggggcggc ttcgccgccg gggactgcgc ggtcggtggg gtcggctgtt
     5821 cgccggcgag cgccacggtg gggcaggcca gcgcggcgtt gtgttccgcc cgggcgaact
     5881 cgcgtgcctc ggcgaaggag tcggccgggt gtgcggactt gccccgctgt gcctcgaagc
     5941 cgtagcggta ggcccagtaa gtcggcagcc tggtcttggc cggtgcggcc gggcgcccgg
     6001 cgaagcagat ctgtggccat tcggcgcccc cggcgtccgg gcgggtgttg gcgacgagga
     6061 gcgcggcgca cgcgcggctg cgcatgcgtg tctcgaccag gccgcagcgc tcggcaacct
     6121 cgtcgaagat ctcctgcacc aggctggatt ccgcgagcca gtcggcctcc cgtcgggcgc
     6181 acaacgcgtc cgcatggtgc aggaagctgc ctgccagcgc ggtgagcttc ggatgctcct
     6241 cgctcatgag tgtcgtcgag cagcggtcgc gtccgggcag ggcggcccag tcctcggtga
     6301 tcttcaggat cgcggcgcgt tcggtcttgc gttcctcggc cagggcgccg atcgcgccgt
     6361 cgatcgcctg ccatgaggac accagcacgg tggccaccgc gccgatgccg atcgaggcgc
     6421 tcaccgcgct gttgcggatg cgctcgggca gccggctggc cgcggcctcg gcgnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngctcacc gcgctgttgc ggatgcgctc
     6601 gggcagccgg ctggccgcgg cctcggcggc ggccgcggcg gccgcgcccg cgcccgccgg
     6661 caccgtcgcc ggggtaacgg aggcccgggg cgtcgcggcg gtgagggcgc cggtgtcgga
     6721 ctcgaacagc gtcgcatggc gactgcgcag cgcctccagc aagtcgctgt cgagcacgtc
     6781 gccgagattg aagccgagct ggtagtagct ctcccactgc gcctcgtcga agaactggtc
     6841 ggcggtggtc tgctgcggga aggccgggtt gctggcggcg aagttgatca ggtcgaccgg
     6901 cagcccggcg ctgatgttgg gcttgaccag caccagccag cccggctcgg tcgcgccgac
     6961 gtagcgcaca cgggcgagcg cgaggcaggc gttgctcttg cgcgaggcca gatcgttgag
     7021 cgagccgaac agctcgaccc cgggcggtcg atcgttctcg cccgctgcgc ggggacgcgg
     7081 gcgcaggaat tcgatctccg cgccgaggtc gatgcgcgcc ttgcgcacca ggttctccag
     7141 gtcgccgaag cggtagtccg ggtcggcgcc gcagtcggcg agcacgatca tgcggcagcg
     7201 ttcggcgagc agggcgtagg cggccgtgtt ctcgaagtgg ccgccatcgc tgaggaacca
     7261 gtcgggtccg ctgctgccct tgaagttgca gaacacctcg ctgagcaggc cgcgtgactt
     7321 ggccgcgagg cggcgtttcg ggcgctgcag gttctcccgt gcagcgcgcg tccaccagta
     7381 gccgaggcga atgccggcga aggccagcag cgccgagatg ccgccgcgcg tctggctgcc
     7441 caggccgggc gacaccgcgg cgcccgagat cgccacccac gaacccaggc cgagggcgcc
     7501 cgcaccggtc acctggaacc acggttcctg gcccacccgc acccagcctt cggggcccac
     7561 cgtcagcggc aggccgcggc ggtcgcggtt gaacagtccg ccgcgcgggt cgtgcgtctg
     7621 attgatgcag accccgatca gatgcaccgg gccgccttgc cgatgcggcg cgtaggcttc
     7681 cagcgcgagg tcatcgtcgg ggtcggggtc gaacacgctc ttgtgggcga ggtgtgcggc
     7741 gccgcgctcg gacacgacgg cggtcggccc gagtgtgtcc tgctggccga agcgggccgg
     7801 attggccgcg cccaggtagc tgcgcaccag gcgcgcgcgg tagaaggtgt gcagcgagga
     7861 caggttgagg aactcgaaat tccgcccggt ggcgagcacg tagccgccgg ccgcagtacc
     7921 gatcgcgagc agccggagcc agggcgtgcc gaagtccacc gtgccctcgg tcgcgaacat
     7981 cggccccagc gccgccgcgt gcacaagcga tacccaccac gcgcacagca ggaagctgag
     8041 tatgtagccg gagagctggc ccaggctcag cagcagcttg ccgctgattc cgccttgggg
     8101 gccgccaccc agcgccggca tcgccgcccg cagcacggcg gcggccaccg ccagggcgag
     8161 cgcggtattg gcctgcgcct tgaactcgaa ggcgaggaac cacgccaccc ggtccaccag
     8221 tccgagcagc agcacgaccg cgatcgcctt catgcacagg gattgccagt cggccagcca
     8281 acgccgcacc gcctcctcgc ggagcctcag cgcatcgcgc tcggtctcgt cgaagaggtg
     8341 gcgcaacagg aagcgcgccc acgggatcgc gatcagccag gcgacggcca ggccgatcgc
     8401 accccaggtc agcgccatcc ggatcgcatg accgggctgc cactgcggac cgatgatggc
     8461 gtcgccgtaa tgcaccaaca ggcccaggac gatcgtcagg atcacgcatt gcacggcgac
     8521 gaagccgggc cgccatcccg gccgcccggc cgacgccacc cagggcacga cccagtacgc
     8581 cgcggtcgcc aggccgccga gcacggccag caccgggatc gcgaccggcc acagcgtggg
     8641 cagccagggg ggcagccagc gcagggcctc gatgcgctcg cccgtcaccc acccgtccgt
     8701 cacccagtgc gcgatcgccg cccacacgcc cacatcgacc gccgccagca ccacgccgag
     8761 cgcgagggcc agcgcgccga gctcgaggtg gatcgcgagc aggttgcgca ggaagacggc
     8821 gagcgcgaag gtcttgtcgg ccggtccgcg cggcaccagg tagcggccgt tggcgcgcag
     8881 ccaccagcga aaccagcgtg gctcctggga gccgaaggcc gcgaagatgg cgcgggtgtc
     8941 gtccgcggag cgtgcgccgt gcagcaggcg gccgagcatg ccgccgatgt agccgccgcc
     9001 ggacacggtc gagagcaggt cgaagcgttc gagcaggcgg ttgcgggcga gcacgccgag
     9061 caggccgaag cagaaggtcg cgctgcggat gccgccgccc gacagggcca ggccccattt
     9121 gtccgcgacg ccgtccttgc gcccgagggc cgcgcgccgt tcgcccacca gttggtcgat
     9181 cttctcgctc atggcaccgt cctccccgcc gctggtgcga agccgcgaac accgtttcgc
     9241 gcgcacagtg tctctatagc acccgaactc tgcgcatgac agcatcaagg acggccgcgg
     9301 cggtgccgcg cggacccggt gcaccagggc cgcggccgtg gcggtcgggg agggcgctgc
     9361 ccgcgaggct cagccggggc cgagccggca cgccgcgtac ttgaactccg gaatcttccc
     9421 gtacggatcg agcgccgggt tggtcagcag gttggccgcc gcctcgacgt agcagaaggg
     9481 cacgaagacc atgcccgcgg gcatcagcgg atcggcgcgg gcggcgagcg tgatgcggcc
     9541 gcggcgggtc tcgatgctca gcgcggcgcc gggggcgagg ccgagtcggg tgagttcggc
     9601 cggggccagc gtcgccaccg cggccggttc gagcgcgtcg agcaccgcgc tgcgccgcgt
     9661 catcgcgccg gtgtgccagt gttcgagctg gcggccggtg atcagcaccg tgggccaggc
     9721 ggcgtcgacc ggctcgtcgg gcggcagcgg cacggtgggg cggaaacgcg cgcgcccgct
     9781 cgcggtgggg aagcggtcgt cgaagaccac gtccttgccc ggagcgtcct cggccgggca
     9841 aggggttgtg accgcgccct cgcgctccag ccgcgcccag tcgatgtggt cgagcgagcg
     9901 catcgcgcgc ttcatctcgg cgaagacctc gcgcgggtgc gcgtactgcc agtcgaggcc
     9961 gaggcgccgc gcgagctgct ggaggatcca ccagtcgggc cgcgcctcgc cgggcagcgg
    10021 cacggcggcg cggccgagct ggatctggcg gttggtgttg gtgacggtgc cgtccttctc
    10081 gggccaggcc gaggcgggca ggatcacgtc ggcgaactgc gcggtctcgg tgacgaagag
    10141 gtcctgcacg acgaggtgct cgagcctggc gagcgcggcg cgggtgtggt gcaggtcggg
    10201 atcggacatc gccgggttct cgccgaggat gtacatgccg cgcacccggc cggcggcgat
    10261 cgcgtccatg gtctcgacga ccgtgaggcc gggctcggcg gggagcggcg tcgcccacag
    10321 ttcctcgaag gcggcgcggt actgcgcgtc gcccaccggg cggtagtcgg gcagcaccat
    10381 ggggatgagg ccggcgtcgg acgccccctg cacgttgttc tgcccgcgca gcggatgcag
    10441 gccggtgccg gggcggccga catggccggt ggcgagcgcg agcgcgatca ggcagcgcgc
    10501 gttgtcggtg ccgtgcacat gctgcgagat gcccatgccc cagaagatca tcgcgcgctc
    10561 ggcggtggcg tagaggcggg cgatggcgcg aatctcgtcc ggcgccaccc cgcacagcgg
    10621 cgccatggcc tcgggcgtga gcggtgcgac gtgggcggcg agcgcctcga agccctcggt
    10681 gcgcgcggcg atgaagtcgc ggtcgcacag gccctcgccg acgatgacgt tgagcatggc
    10741 gttgaacagc gcgacgtcgc tgcccggcgt gaagcgcacc atgcggtggg cgtggtggcc
    10801 gagcaccagg ccgcgcgggt cgagcacgat cagcctggtg ccgcgcttgg cggcctgctt
    10861 gaagtacgtg gcggcgaccg ggtggttcac cgtcgggttg cagccggtga ggatgaccac
    10921 gtccgcgtgc gcggcctgca tgaaggaggc cgtgaccgcg cccgagccga tgcactccat
    10981 cagcgcggcg accgagctgg cgtggcacag ccgggtgcag tggtcgacgt tgttggagcc
    11041 gaagcccgtg cgcaccagct tctggaacag ccaggcctcc tcgttcgagc acttggcgct
    11101 gccgaagccg gcgagcacgc cggggccgtg ggcgtcgagc aggcggcgca ggccggcggc
    11161 ggcgaagtcg agcgcctcgt cccagctcgc cgggcggaag tggctgagcg gattcgccgg
    11221 atcgaaggcg ggatccacgc ccttgggcac gcccgcgcgg cggatcagcg gcgtcgtgag
    11281 gcggccgcgg tggttggggt aatcgaagcc gaagcggccc ttcacgcaca ggcggttctc
    11341 gttggccggg ccgttgcggc cctcgacgaa gaggatgcgc tcgtcccgga cgtggtaggt
    11401 gagctggcag cccacgccgc agtaggggca caccgaatcc accttgcgct cggcgcaggc
    11461 cgagtcgccg cggccctgcg catcgaccag ggtggcgggc atcagcgcgc cggtgggaca
    11521 ggcctgcacg cactcgccgc aggccacgca gctgctgtcg ccgagcgcat cgtcgaagtc
    11581 gaagacgatc ttcgcgcgtg caccgcggtg ggcgaggccg atgacctcgt tcacctgcac
    11641 ctcgcggcag gcgcgcacgc acaggccgca ggtgatgcag gcgtccaggt tcacgtggat
    11701 cgcggggtgg gtgcggtccg gctgctgcgc tgcggggccg cggtcggcgg cgggcaaacg
    11761 catgtgctcg accagctccc agaaatggct ggagcggtcg gggctgtgcg tgcgtgccgg
    11821 ctgctgttcg agcagcagtt cgagcacgcc ctcgcgcgcc tgtcgtgccc gcgtcgagct
    11881 ggcgctgcgt accaccatgc ccggctgcgc cgcgcggatg caggaggcgg cgagcacgcg
    11941 ctcgccctcg atctcgacca tgcaggcgcg gcagttgccg tcggcgcggt agccgggagc
    12001 gtccttgaaa cacaggtggg ggatgcgttc accggcgcgc agtgcgacct gccacagcgt
    12061 ctcgccgggg aaagcctgcg ccgtgacgcc gtcgaggagg atctcgaaag cgtccgcacg
    12121 ctggtcgagc gcgtcgtgtg ctgccgtacg atccggcgcg gtcgccaggc cggggggctg
    12181 gggcgtgggg ctgcggtcct cgcgatcgtg ccggctcatg gctgcagctc cccggcatcg
    12241 cgggcaggag gcccggcatg cagcctcacc ccggcgcgcg cgagctcggc cgggaagaag
    12301 cgcagcaggc tgctcaccgg gttgggcgcg gcctggccga ggccgcagat cgaggcgtcg
    12361 cgcagggtct gcgcgagggc ctgcaggcgc gccacgtccc agtcgtccgt gcgcagcagc
    12421 gcgagcagct tttcggtgcc gaggcggcag ggcgtgcatt ggccgcagga ttcgtcggcg
    12481 aagaaggcga gcaggttgtc ggcgacggcg cgcagatcgt cctggtccga gagcacgatg
    12541 atcgccgccg agccgatgaa gcagccatgg ggctgcaagg tgtcgaagtc gagcggcagg
    12601 tcggcgagcg cggcgggcag gatgccgccc gaggcgccgc cgggcaggta ggcgagcagg
    12661 cggtggccgg gctgcaggcc gccgcagtgc tcgtccacca gctcgcgcag ggtgatgccg
    12721 gcgggggcca ggtgcacgcc cggcttgttc acccggccag acaccgagaa gctgcgcagg
    12781 ccgccgcgcc cgcgccgccc ctcgccggcg aaggcggcgc cggcggcgag cagcgggatc
    12841 cagtacaggg tctcgacgtt gttcaccagc gtcggccggc cgaagagccc ggcctcggcg
    12901 acgaagggcg ggcgatggcg cggcttgccc ggcttgcctt cgagcgactc gatcagcgcc
    12961 gactcctcgc cgcagatgta ggcgccggcg ccgcggcgca acacgatgaa gccgggtgcg
    13021 acgaggccgg cggcctccag ttcggcgatc gcctcgcgca gcaccgcgtg caggccgggg
    13081 tattcgtcgc gcaggtagac gtagatcgcc gccgcgccca ccgcgagcgc gctcgccagc
    13141 gcgccctcga gcacccggtg cggcgccgtt tccaggtagt ggcggtcctt gaaggtgccc
    13201 ggctcgcctt cgtcggcatt gacgaccagg tagcgcggcg cggcgccggc gcgcaccgcc
    13261 tgccacttgc gcgcggtcgg gaagccggca ccgccgaggc cgcgcaggcc ggcttcggcg
    13321 agccgggcct cgagcgcggc aacgccggtc tcgccgctgc gcaggcgctg cagcagggtg
    13381 tagccgcctg cggcgcgata gtcggcgagg cgctgccagg cgatcggttc ggggtcgagc
    13441 gctccgcggg cgagtgcggc caggaccgcg gccggggtcg cgcggctcag gtgacggcgg
    13501 ccgagctggg cgaccggggc ctggtcgcag cgtcccatgc agggcgcgcg cagcacgcgg
    13561 atgcgcgccg gatcgagcgc ggcgtcgagc gccgcgcgca gcgcctgcgc gccggcgagc
    13621 tggcagggca gggagtcgca cacgcgcacg gtgagcgcgg gtggcaccgg ctcgtcctcg
    13681 cgcacgacgt cgaagtgggc gtagaaggtg gcggtctcgt acacctcggc catcggcatc
    13741 cgcatccagt cggcgagcgc gcgcaggtgg cgcagcgaga ggtggccgaa gcggtcctgc
    13801 agcgcatgca ggtgctcgat cagctcgtcg cgctcgcggc gggcagcgcc gagcagggcc
    13861 tcgatctcgg cgagcgcggc gggatcggtc tggcgtccgc gcaggccggg gcgggtgtgc
    13921 cggcctgcgg cttgggaggc ggagggggag ggggagggag aaggcatggc gatcggatcc
    13981 ggggtgctgc gggcgcgggc tcagcgtcgc ccgacgagcc gcgcggcgcc ggtcacgatg
    14041 cgcgccatca acgcgcgcac ggcgtctccg ccgggggagt cgagcgtcgg cgggcgccag
    14101 gcgagggtgg cgtcgcgcgg ggtgtgcggc agactgtaca ccgcgatcga tagcgggcac
    14161 agcgcgatgt gttcgcgcgc ctcggcgacc agacgggcgg cgacgccggc actgcagaag
    14221 ctgagtacct cggcgtcgcc gtagagcgcg gggtcgtggc cgagatcggg tgcggtgcgt
    14281 tcgagcatgg cgccgaagcg gctgaccacc ggcgcgctca ggccctcgtc ggcgatcgcg
    14341 tcggcgaggg cctcgcgcag gtcggcgaag ggcacgccgg ggaagtgctc gaccgcgatg
    14401 ccttcggccc gcgcgctgga gctggtcgcc ggagcgagcg cgagcgcaca gcagatcgcg
    14461 gccaggcgca gggcgcgcag cagtcgtcgc ggcacggcgt gggccgggga cgccgctggc
    14521 gtgctggtgt gcgtagctgt gcgcgccggc cgcggatccg tccgtgcgcg gccgcgtgcg
    14581 acgtcgcgtc gcgctacggg cccgcttgtt gcttcgttca tgtcctcacc tcgaccggag
    14641 ccgcaacgcc atgcgccctc tcgttgcacg attgttgacc gcctggatca ttgcacaggc
    14701 gcccggcgcg gcgcacgcgc tcgaacccga ggagctcgac gcgctggtgg cgcggcccag
    14761 catcggcctc tacaaggcgt acgccgaatt caagatggcg cgctacggca gcgcgcgcga
    14821 gatctggagc gcgctcgcgc aggccggcgt ggcggaggcc tggttccacc tcggcatcct
    14881 cgccgaggat ggcctgggcg agccgcgcga cgccgccgcg gcgctggagc gctaccgccg
    14941 cggcggcgag gccggctcca gcaaggcgca gtaccggctc ggcctgctcc atctcgaggg
    15001 ccgcctggtg ccggccgatc cggtgcaggc gcgctactgg ctggcggtgg ccgctgccaa
    15061 cggcgacgag gaggccgcac gccggctcgc cacgttgccc gcgtcgatcc ccgccgcgcg
    15121 ctgagcgcgc aagccggcgc gcacccgcgc gcgggacgct cgcgtggccg cgccggcgac
    15181 cgtcccgccg caccctcggc gtgatcgcgc cgcgctcagc gcgacgcctt gttgcgcgcg
    15241 gcttcgagca cggcctgctc cgccgcgggg aagaggtgca ccaccgagcg gcggaactcc
    15301 gcctccacca gcgccaggcc ggcgaagcgc tgcgggatcg gggtctggaa gagctcggtc
    15361 atgtcctggc ccgactcggc gccggcgcgg atggcctgcg ccagccagcc gaggtagtcg
    15421 cgggtctggc gcagcggggc gaggtcggtc gccggctcgc catgacccgg cacccagcgc
    15481 cgcgcgggca ggccttccag cgtgtcgagc gcggccagcc agcgcgcgat gtccgcgtgc
    15541 ggcgtggtgg gcgcgcggcc gttgaagagc aggtcggcgg cgtacacggt gccgctgtcg
    15601 tggtcgatca ccaccaggtc ggcgacggtg tggccgccga gcgcgaccag ctccacgtcg
    15661 cgcccgccga tgcgctggcg cccggcggcg agtgcgcgct cggggacgac gacctcggtg
    15721 tcgcgcatcc agtcgccgtt gaggcggtac atgttggcgt tgaagccttc gccctcgctg
    15781 cggagcgcgg cgatggtctc cggcagcgcg gcgagcgtcg cgcgcgggaa ggcctggttg
    15841 ccgaggaagt ggtcggggtg gtggtgggtg ttcaccgtca gcacgatcgg caagggcgtg
    15901 acgcggcgga tggcggcgag catctgctcg ccgtagcgct tcgacgagcc ggtgtcgatg
    15961 acgatcaccc cctcgcggcc gacgatgaag ccggtattga cgatgttgcc gccgttggcg
    16021 aaggagaaat cctccttcag gccgaccacg acccaggcgc cgggggcgag ctcgcgcggc
    16081 tgcaggcggt agttgaagtc cccggcgaag tcctcggcgt gcgccggcag ggcggtgagg
    16141 agcagggcgc acagcgcgac cagcagcccc gcgagcaggg tccgcagcgc gcgggcggca
    16201 ggacggcgct ggagcgccgg agcgcaagag gcgggtcggc tcatcgggtc acccatgcgt
    16261 cgatgcggtt gccgttgttg tcgcgcccgg ccacgtgcag gcgcgcgccg ggcgcggccg
    16321 cgcgcaggtc gagcgtgaag ctggggttct cggagaccgg ctcgaggggc tggatgcggc
    16381 tcagcgtgcg gccttcggcg tcggcgatca cgatgtcctc gatgtggaag gcggggatgc
    16441 cggcggccag gccggtgtcc atcggatgga tgatgcgcat gcgcaggcgc tcgccaccgt
    16501 cgacccgggg ccacagcttg ccgttgacct cgttgaggcg ctcctgccat tccggcgagc
    16561 ccgagcccac cgagggcagc gtgcagccgc cgccggtggc cgtgatccag gcgccgccga
    16621 cgtgccacac cccgtcggcc gtgcgcgcgg cggcgcgcac cggcgaggac tgctgcagct
    16681 tgatgcggaa gcccaggcgc ggctgggcgg cgccgggctc gaagcgcagc gccttgacga
    16741 tcgggttgaa gtcggcgaac acgatcagct cctccacccc ggggagggcg ctcgcatcga
    16801 cattgaccgg gacgttgagc gaatcctccg cgaccgccgg gccgctcacc ttgacgcgct
    16861 cgtcgaacac caccgtgacg tccgcgaaga agctcttgcg catgtcttcc cagcgtggcg
    16921 agtcgagcgg gtcggtgtgc gcggcggagg gggagggggc gcccgcgcgg ggggctgcgg
    16981 cgagcgctgc cgggctggcc agcgcggtca gggccgcggc gatccaggcg agggcggggc
    17041 gggcggacgg tctcatcggt gctcctgtgg gccgggcgcg gtgcgtgcgc ctgcgtgtgc
    17101 cggagcgtgg atgcaagcca catgccaatt ccgcgagcgg gccacggacg cggacctgcg
    17161 cagccgcgga gcattgcgcc cggatcccgg gtgttccgat ccgcaacatc ctgtgctccg
    17221 ccgccacagt gtccgtccgc aatgcgcccg cgttggagca gcgcgggcgc gagcgcatgg
    17281 gttcgtggcc ctggcacggg ctttgcggtg gggtcgtggt ccggcacgag ttggtgccgg
    17341 aagtcacacc acccggaaaa gcttagaaag aaatccaagg aggagtcgat gatgaagaac
    17401 gacagacgcg cgcgcaccct gccggcgatg ctgaccgccc tgacggtcgc gctggccgcg
    17461 gcgggcatgg gcgccacggc gcaggccgcg agcgtgagcg acgaggacat cctgaaggat
    17521 gccgagacga cgcatcaggt cgtcaccaac ggcctcggca ccaagggtca gcgctacagc
    17581 ccgctcgcca aggtcaatgt cgacaccgtg aaggacctcg tcccggtgtg ggccttctcc
    17641 ttcggcggcg agaagcagcg cggccagcag tcgcagccgt tgatccacga cggcaagatg
    17701 ttcgtgaccg cgtcctacag ccgcatctac gcgctcgacg ccaagaccgg ccagaagctg
    17761 tggaagtacg agcaccgcct gcccgagggc atcatgccgt gctgcgacgt gatcaaccgc
    17821 ggcgccgcgc tctacggcga cctggtgatc ttcggcaccc tggacgcgca gctcgtcgcg
    17881 ctcaaccgcg acaccggcaa ggtggtgtgg cgcgagaaga tcgacgacta caaggccggc
    17941 tactcgatga ccgccgcgcc gcagatcgtc aagggcatgg tgatcaccgg ggtgtcgggc
    18001 ggcgagttcg gcgtcgtcgg ccgcgtcgag gcgcgcgacg ccaagaccgg caagaaggtc
    18061 tgggtgcgcc cggtggtcga aggccacatg ggctacaagt acgacaagga cggcaaggag
    18121 atcgagaacg gcatctcggg cacgaccaac gccacctggc cgggcgacct gtggaagacc
    18181 ggcggcgccg cgacctggca gaccgcctac tacgatccgg acgtgaacct gatcttcatg
    18241 ggcaccggca acccggcgcc gtggaactcc tggctgcgtc cgggcgacaa cctgtactcg
    18301 tcgtccacgg tcgcggtcga cccggacacc ggcaagatcg tctggcacta ccagaacacg
    18361 ccgcacgacg gctgggactt cgacggggtg aacgaattca tctccttcga gtacaaggac
    18421 ccgaagaccg gaaagctggt caaggcgggc gcgaaggcgg accgcaacgg cttcttcttc
    18481 gtcaatgacc gcaccaacgg caagctgctg ggcgcctacc ccttcctgac ccgcatcgac
    18541 tgggccaagg gctacgacct cgagaccggt cggccgatca ccaacgacga caagcgtccg
    18601 cccaatccgt ttgccgaggg cgcggccggc gagggcggca agggcaagcc ggtgttcgcc
    18661 gcgccgtcct tcctcggcgg caagaaccag atgccgatgg gctacagccc ggacaccggc
    18721 ctgttctacg tgccggccaa cgagtggggc atggacatct ggaacgagcc ggtgagctac
    18781 aagcgcggcg cggcctacct gggcgcgggc ttcaccatca agccgctgta cgaggactac
    18841 atcggcgcga tgcgcgcgat cgacccggtg agcggcaaga tcgtctggga ggtcaagaac
    18901 aacgcgccgc tgtggggcgg ggtgctcagc accgccggca acctggtgtt ctatggcacg
    18961 cccgagggct acctgaaggc ggtcaacgcg aagaccggcg aggaggcgtg gaaattccag
    19021 accggctcgg gcgtgatcgc gccgccggtg acctgggaag ccgacggcga gcagtacgtc
    19081 gcggtggtgt cgggctgggg cggggcggta ccgctgtggg gcggcgacgt cgccaagcgg
    19141 gtgaacttcc tcgagcaggg cggcaccgtg tgggtgttca agctgcacaa gagctgatcg
    19201 cgttgcctgc ggcgccctcc ggggggccgc aggccgcggc gccgcactcc ccgcggcccg
    19261 gacgcgatgg cgcccgggcc gtgtcgtgtc gggacgccgc atgcgtggct gcggtcgcgc
    19321 cgcgagaaag aggcaggttt ttcggcaaca tggagcattt ccgatgacaa agttacagct
    19381 atccagcttc gccaccgcgg tcgtgctcgc ggtcggcgtc ggtacggccg cgcatgcggc
    19441 cgagcgcgcg acaccgcgcg aggcgcgcac gatgttcgag caggccgtgc gctacatgga
    19501 ggagaacggc cccgagcgcg cgttcgcggc cttcaacgat cgccagggcg ggttcgtgcg
    19561 caaggacctc tatgtcttcg cgatcgacga caagggtgtg tatcaagcca gcggcgcggc
    19621 gcccgaggcg ctggtcgggc tgaaggtgct cgacaccacc gacgcggcgg gcaatccgct
    19681 gttccgcgag atgatcgacg ccacccgcgt cagccaggag gcgacggtgc gctacgtgtg
    19741 gctgaaccgc gcgagcaaca aggtcgagcc caaggtcagc tatgtgcgca aggtcgggca
    19801 gtacgtggtc ggggtgggct actcggcgcc acgcgcgagc gcggacgatg cgcgcgcgat
    19861 gctcgagcgt gcggtcgcgg tggcgcgcgg cgagggtggc gcgcgggccg cggcgcgctt
    19921 caacgaccgc cgtggcgact tcgtcaagga cgacctctac gtgttcatgg tgaacatgga
    19981 gagcggccgc ttcgaggcga tgggcatgaa ccccgcgctc tcggacaccg acgcgctcgg
    20041 cctgcgcgac gccgccggca atcccctgat cgccgagatg atcgcccggc tgcagcaggc
    20101 ggacgaggcc accgtcgatt acgtgtggcg caatccggtc accaacgcgg tggagcacaa
    20161 gcgatcctac gtccgcaagg tgggcaagtc cctggtcggc gtggggcact atctcgagta
    20221 agtgcagggt gttgctccac catggagcac atgctccgtg gtggagcaat gtggagcaga
    20281 aaaaagggcc cgcgatgcgg gccctcttca ttggcgggtg gattgtcgga gcgaatctgc
    20341 gcggctgggg cggcgtgggc gtgcgagccc ggctgccccg aaggatcagg gggtgcgctg
    20401 tctgaccccg tgcgcggcga agatcctgcc cagctcgccg gagcgctgca gctcggcgat
    20461 cgcccccgac aaggcctgct ccaggccgct gtgctccgcc ttgacggcca tgccgagcgg
    20521 ccaggaggcg acgcgcagct cgggcatcga cagggtgtcg agcgcgatcg cggcgtcgcc
    20581 gccgatcgcc gtctccagct cggagcgcgc gcccatcacc gcggcgatct cgccgcgctt
    20641 catcgccgcc accgcctcgc ccacgttgcg gaagtgcgcg acgttgggct gcaggcgccc
    20701 gcccaggatg tggcgcagga agtcgctcgc atgcgagtcg agctcggcgc cgatgcgttc
    20761 ctgctcgaac accgcgaagc tggcgtcggc cgagcccccc ggtgcgggca cgcgctcggg
    20821 caggcgggcg agcgcgatgg tctcgaggtg gtaggggccg aagatcttca cctgcttgtt
    20881 gagtgctgcg aagcgcgggt cgacgggcac gtgcagcatg acgtcgcccg gcttgtggcc
    20941 gaggtagtgg ccgcgccaga ccatgttgcg caggtcgtcg ttcatgttct cgtcggcggg
    21001 aaactccacg acctgcggct gcaggccgag gcgctcggcg agcgcgcgcc cgagtgcgat
    21061 atcgatgccc ttgccggcgg tcgaccaggg cgcgaagttg gcatagaccg cgacgcgcag
    21121 cgtgcccggc tgctgcagct cgagttcggc cgcggccgcc cgcgccgggc tggcggcaag
    21181 gacggcgagc gcgccggcgg cgagcaggaa acggcggcga tcagttttca tggacggtct
    21241 ccagccaggc gcggatggcc cacatggcct cctgggtcag catgccctcg aagggcggca
    21301 tatagaccgc gccgttgcgg gtgacaccgt tgcgcacctt gccgatgaag aactcgtcgg
    21361 tgccgtcgtc ggcgacgtcg agcaggcgca ggtcgggggc gatgccgccg gagaccgcgc
    21421 cgaggccgtg gcagcgcgcg cagttctgat tgaaggcgga gtcgccgatg cggatcgcct
    21481 ccttgttgcc gcggtagggg ttcaccgtga cccaggtccc gccgagctgc ttgagcgagg
    21541 acgtgtcgac cgcctgcggc gtgacgtcgc cgtgggcatg ggccagcgcg ctggcggctg
    21601 caagggcgat cccggtcgcg gtgatggcca ggcgcttgcg caggttctcc ttgatggtac
    21661 gagtgttcac cttgtcttcc tcttcgatgt ggtggtgttt ggatccgctc cggttcaccc
    21721 cggatgggag tcctactgca atccctgtgc catgtgcgtc cggctgccca ggcatggacg
    21781 gccccgggtt ccggccgcca tgccgcggtc ggcctggcag gattcctgct tgctccttcg
    21841 ccggcggagc agcggatttg tatcgatccg catcaggtgc tacgttttga atcgaagtcg
    21901 aagagtcaca agaggcggtc gccggcacgt cgcgcggcaa ggctgcccgg aggagacatg
    21961 aaagtcatgc aacagaccgc gatggcgccg gccgtccgcg aggacgcggt ccggctggca
    22021 cgaggccagt ttttcgggga agggcgtgcg cccgaggcag gcgtgtcccc gctcatcgtc
    22081 gactcgtggc tgcgttgccg cgggcgcgga atggcagccg acgaccgcgc cgaagccgag
    22141 catgcgggtc gcctgcagct ggcggaggcg cgcgaaggca accgcagcct gctcgattac
    22201 gccagtgggg tcatggaaca cgtgttcgag cagatccgtg cctcgggcag cctggtgatc
    22261 ctgtccgacc cgcagggcat gatcctgcac agcctgggcg acgcggagtt cgtcgatcgg
    22321 gcgagccggg tggcgctgca gccgggcgcg tgctggagcg aggcggcgcg cggcaccaac
    22381 gccatcggca cggcgatcgt gaccgaagcc cccaccgtgg tgctcggcgg cgagcactac
    22441 ctcgaccgca acggcttcct gtcctgcacg gccgcgccga tccgcgatcc gcgcggcgcg
    22501 ctcgccggcg tgctcgacgt gtcctgcgag cagcgcatgc accagcgcca ctcgctcgga
    22561 ctggtacgct tgtccgtgca gctgatcgag aagcgcctct tcgaggccga gttcgccaac
    22621 cacatcctgg tcgccttcca cgagcgcccg gagtacgtcg gcagcctgca ggaaggactg
    22681 gttgcggtga cgcctgaggg ccacctggcc ggtgccaact cgatcgcgcg cgagtggatc
    22741 gggcgtcgcg tcgagtgcgg aggtggggcc tcgttcgcgg agctgttccg ccaggggctc
    22801 ggaaaggtgg tcgaccgggc gatgaactcg agcgacgcgc tgatccggct gggactgcgc
    22861 gatggttccg agatccacgt tcagctgcgc tcgctgcgcc cgcgcttcac cgccttcggc
    22921 tcggcccagg ccggggaggg catgcgcacg tttgccgcgg aatccagccc ggcggcggtc
    22981 ggctccgcac ccccggtgtc ggcgccggcg cctgtgtcgg cgccgcacgg gccgatcacg
    23041 ctccacgatc tcgccaccgg ggacgcgggt atcgggcggg cggtcgatcg tgcgcggcgc
    23101 atcctgggca aggacattcc gctgctgatc cagggcgagt cgggcgtggg caaggagctc
    23161 ttcgcccagg ccttccacaa cagcgggccg cgcgccgaca agaccttcgt cgcgctcaac
    23221 tgcgccgcgg tgccggagac cctgatcgag tcggagctct tcggctacgt cggcggcgcc
    23281 ttcaccggcg cgcgcaagga aggcgcgctc ggcaagatcc agcaggccaa cggtggcacg
    23341 ctcttcctcg acgagatcgg cgacatgccg ctcggcatgc aggcgcgcct gctgcgcgtg
    23401 ctgcaggagc gctgcgtgac gccggtgggc tcggtcaagt caatcccggt cgacatctcg
    23461 ctggtgtgcg cgacccaccg cgtgctgcgc gatgcggtag cgcgcggcga gtttcgcgag
    23521 gacctctact accgggtcaa cagcctcacc gtgaccctgc ccgcgctgcg cgagcgcagc
    23581 gacatccgct gcatcgtcga gcgcatcctg gccgcggagt gccgcgaggc cgggctgcag
    23641 ggcgtgtgga tcggcgacga ggtcatgcgc ttcgtcgagc gccacggctg gccggggaac
    23701 gtgcgccagc tgcagaacgt gatccgcgtc gcggtcgcgc tgctcgacga gggcgagcgc
    23761 gagatccggc ccgagcatct gcccgaggac ctcttcctcg ccgatccggt cgacggcggc
    23821 aagacgccgc cggcggccca cgcggggccg ctcgtggcgg cggcgagcgc cgcgctgccg
    23881 ctggcggccg acgcgccgcc gatttccgcc gcgaaacgcc tggaccagct cgagatcgag
    23941 ttgatcgagc gcgtcatgcg cgaggtcggc ggcaacctgt cggcggcggc gcgcacgctc
    24001 ggggtcagcc gcaaccgcct ctatcgcaag ctcggccgcg ccggcggggt gtcctgagtg
    24061 gggtaatccc gccttgcccg cgggcaggta tgacttatgc ttgtacttgc ataggtgagc
    24121 agtggggcgt actgcccgca gcactgtgac cgagcgcagg aatcgataaa cgacaaggag
    24181 acgatcgatg tggaaagcac tgcacattga tcccgcgaag tgcaccggct gtctgcaatg
    24241 cgagatggcc tgttcctacg agcacaccgg ggtcatcaat ccgagcaagt cgcgcatcaa
    24301 ggtgttcaac ttcgagcacg agggccgcaa ggtgccctac acctgcacgc agtgcaccga
    24361 ggcctggtgc ctgaactcct gtccggtgga tgcgatccgc atcgacctgt cgaccggcgc
    24421 caagatggtg ttcgaggaca cctgcgtcgg ctgcaaggtg tgcacgatcg cctgtccgtt
    24481 cggcaccatc aactacaacc aggacagcgg caaggtgcag aagtgcgacc tgtgcgaggg
    24541 caaccccgcc tgcgccagcg cctgtccgac cggggcgatc acctatgtgg acgccgactg
    24601 gaccggcatc gacaagatgc gcgcctgggc cgcgaaggcc aacaccccgg cttcggccgc
    24661 tgcctgagga ggatgcgtca tgggatggaa tcgcaaagtc ctgcgcgtga acctcaccgc
    24721 agggacctgt accgaagaag cgctgaacat gcagtgggcc gacgaatacc tcggctcgcg
    24781 cgggctggcc accaagtacc tggtgtccga gatcgacccc agggtcgacc cgctgtcgcc
    24841 cgacaacaag atgatcatgg ccaccgggcc gctcaccggc accatggcct cgaccggtgg
    24901 ccgctacacc gtggtcacca aggggccgct gaccggcgcc atcgcgtgct cgaactcggg
    24961 cggcttcttc ggcgccgaga tgaagttcgc cggctgggac atggtgatct tcgagggcaa
    25021 gtcggccaag ccggtatggc tctacatcga gaacgacaag gccgagctgc gtgacgcttc
    25081 cggcctgtgg ggcaggagct gctgggaaac cgaggagaag atcaaggagc aactccagga
    25141 cccgctggtg cgcatctcct cgatcggcgt ctcgggcgag aaccaggtgc tctattcctg
    25201 catcgtcaat gacctgcacc gtgccgccgg gcgctccggc gtgggtgcgg tgatgggctc
    25261 gaagaacctc aaggcggtgg cgatccgcgg caccaagggc gtctccggca tcaaggactt
    25321 cggcgccttc ctcaaggcca cgaacgacgc caagaaggtg ctcgccgaca acgcggtcac
    25381 cggccagggc ctgcccaagt acggcaccca ggtgctgatg aacgtgatca acgagatcgg
    25441 cgcgctgccc acgcgcaacc accgcgacgt gcagttcgag gacgcctcga agatctccgc
    25501 cgaggccatg cacgagaagc gcgcgtccga cggcaagacc cacctcgtca ccaacgccgc
    25561 ctgcttcggg tgcacgatcg cctgcgggcg catctccgag atcgacaaga cccacttcac
    25621 ggtcaagaac agcccgaagt actggggcgc ctccggcggc ctggagtacg aagcggcgtg
    25681 ggcgctcggc gcggccaacg gcgtgggcga cctcgaggcg ctgcagtacg ccaacctgat
    25741 ctgcaacgag cagggcatgg acccgatctc cttcggcgcc actgtcggcg cggcgatgga
    25801 gctctacgag ctgggcatcc tggacgacgc gaagatcggc gtcgcagcgc ccttcggctc
    25861 ggccgaggcg ctggcgaaga tggtcgagat gaccgcgctc ggcgagggtt tcggcaagga
    25921 gctcggcctg ggcagcaagc gcctgtgcga gaagtacggc cgccccgagc tgtcgatgag
    25981 cgtgaagggg caggaattcc ccgcctacga ctcgcgcggc atccagggca tgggcctggc
    26041 ctacgccacc tccaaccgcg gcgcctgcca cctgcgtggc tataccgtgg cgtcggaggt
    26101 gctcggcatc ccggtcaaga ccgacccgct caccaccgat ggcaagcccg agctggtgaa
    26161 ggccttccag gacgccaccg cggtgttcga ctccgccggg atctgcgtgt tcaccagctt
    26221 cgcgtggacc ctggccgacg tgcagccgca ggtcgccgcg gcgtgcgagg gcgactggag
    26281 catggagaag ctcaacgagg tgggcgagcg catctggaac atggagcgca tgttcaacaa
    26341 cgccgccggc ttcaccgcca aggatgacaa cctgccgccg cgcctcaccc aggaggcggc
    26401 gaagacgggg ccggccaagg gcctggtcaa cggcctggcg aagatgctgc ccgagtacta
    26461 tcaggtgcgc ggatggacgc cgggtggcga gccgaccgcg gagacacggg cacgcctggg
    26521 cctgtgattc agcaaacgcc catggacgcc cccgcgggcg cccatggcgc tggattgccc
    26581 tgcaggagcg gcgtcagccg cgagggtctg gacgggcgcg gttggcgccg cgcacgctcg
    26641 gcccgatccg aagggcgcgc ggcttgcgcc gctcctacgg ggcccggatc acccggatta
    26701 cctgcgatcg ccttgccgcg gcctgccttc cacgccgcgg ttcgctagag gacgctgcaa
    26761 tgaaacacct gattctcggc aacggccccg cgggcgtcgt tgctgccgaa accctgcgca
    26821 aggtggcgcc ggccgacgac atcctgatgg tcggccacga ggacgcgccg ccgtattcgc
    26881 gcatggcgat tccctacctg ctcgaaggca acatcgacga gtcgggcacc tggctgcgca
    26941 aggaccccgg ccacttcgag cgcctgcgca tccgccagct gcgcggccgc gcggtcgcgc
    27001 tcgacgccga caagcgcaag gtgctgttcg acgacggcca cttcgagcac tacgaccgtc
    27061 tgctgatcgc caccggctcg catccggtgc gcccgcccat ccccggcgtg gacctgccct
    27121 cggtgcacac ctgctggacg ctcgccgacg cgcgcgcgat cgccgcccat gccagacccg
    27181 gcgcgcgcgt gctgcaactg ggcgccggct tcatcggctg catcatcatg gaagcgctcg
    27241 ccaagtgcgg cgtgcagctc accgtggtgg agatgggcga ccgcatggtg ccgcgcatga
    27301 tgacgccgaa ggctggcggc atgatcaagc gctgggtcga ggagaagggc atccgcgtgc
    27361 tcacctcggc gggcgtgcag cgcatcgacc gcggcagcga ggccggttcc gatgcgccgc
    27421 tggtggtgag cctgtccacc ggcgaccgcc tcgactgcga cctggtgatc gtcgccgcgg
    27481 gcgtggcgcc caacgtcggc tttctcgacg agacgccggt gcatgtggcc aagggcgtgc
    27541 tggtcgacga ccacatggag accagcgtgc ccggcatctt cgcggccggc gacgtggccg
    27601 aggcgcccga cctgttcacc ggcgcgcacc tggtgtcggc gatccagccc aacgccgccg
    27661 accaggcgcg catcgccgcc accaacatgg cgcgcgaccg cgtccacgag tcgcgcctca
    27721 acggcgtgct ggccatcaac gtgctcgaca cgctcggctt gatctcctcg tccttcggcc
    27781 agtggtgggg cgaaaagccc gaggccggcg gcagcggggt cgagatggtg gacgaggcgc
    27841 gctaccgcta cctgagcctg cagttcaagg acgacgtgct ggtcggcgcc acctcgatcg
    27901 ggctcaccca gcatgtgggc gcgctgcgcg ggctgatcca cggccaggtg aagctgggcg
    27961 actggaagga gcgcctgctc gccaacccgc tcgccttcgt cgatgcctac gtcgcgcgca
    28021 gccagcagcc gatggcgctg gcgcgctgag gggacgatcc tggagactcg cgtgacgatc
    28081 cgcatcgcct tcaagctctt cgcctcgctg tcggactatc tgcccgacgg ccaccgtggc
    28141 aaccgcctcg aactcgaggt ggacgagggc accacggtgg cggagatgat ccggcgctac
    28201 aacgtgcccg agcgcagcgc gcatctggtg ctggtgaacg gacacttcgt gccgccctcg
    28261 gagcgcgccc gcaagcgcct cgccgacggc gacgagctcg ccatctggcc gccgatcgcg
    28321 ggcggctgag caggtggcgt tcatcaatgg cccggtgccc gagtccttcg agcgccgggt
    28381 caccgccacc gcgcgcgagt tcgagcgcga cctgcgccag gcctggccgg gggtggaggg
    28441 cggcgcggca agcctgcgct tccgcctgca ggacggcgcg cagcgcctgg tcatcgacat
    28501 cgagcctgcc ggcgtgcgcc gcctgggtct gttcgagctg ccgcaactga tcgtgcgcta
    28561 cggcttcggt ccgggcgagg aagccgcgcg ccgcgccttg ctggcgcggc tcgatcgcgc
    28621 gatgcagaag ggcggcggtt gatctgcagc acaatcgatt tctggaagcc cgtccatgag
    28681 tgaagcatac gtcgtcggcc atatcacggt gaagaacgcg gagatgtggg ccgaataccg
    28741 cagcaaggtg ccgcagacct tggccccctg gggcgcggag ctggtgtttc gcggccggca
    28801 ggtggccgcg ctcgcgggcg aaagccccca cccggacatc gtcgtgatcc gctttcccag
    28861 cgtcgcggcg gtgaacgact ggttctcgtc gccagcctat caggccctga tcccgctccg
    28921 ggagcaggcg gcggacgtgc tcctgctgtc ctacgaaagc tagccgcggg cgcctttcgc
    28981 accggcctcc gacaccagcg cgcgccgcgg cggcgcccgg ctgaagccgc tcccggcgtt
    29041 ccggccggcg tcggctttcg gttatcctcc ccgccgatgt ccgccttcgc cgccaccttc
    29101 tccgatgcgc tgtcgctgct tgcccatttc gaccacacgg tgctcgagat cgtcgcgctg
    29161 tcgctgcagg tcagcggcgc ggcggtggtg ctcggcacgc tgatcggcct gccgctcggg
    29221 gcctgcatcg cggtcgggcg ctttccgggc aagaccacgc tgtcggtgct gatcaacggc
    29281 atgatgggcc tgccctcggt ggtggtgggc gtggtggtgt acctgatcct gtcgcgctcg
    29341 gggcccttcg gcgccctggg catgctgtat tcgccggcgg cgatggtggt ggcgcagacc
    29401 ctgctggtgg tgccgttgat ggcggcgatc acccgccagg tggtcgagga cgtgtgggcg
    29461 cgctacgccg aggagctgca ggtgatgcgc ttttcgtggt gggacagcgt caccacgctg
    29521 ctgcacgact gccgccactc gctggtggtg gcggtgctcg cgggcctcgg acgggcgatg
    29581 agcgaggtgg gcgcggtgat gatcgtcggc ggcaacatcg acggcgccac ccgggtcatg
    29641 accacggcga tcgcgctcga gaccagcaag ggcgacctgg cgctggcgat cgcgctcggc
    29701 atggtgctga tcgtgatcat cctcgcgctc aacgcgctcg cctacgtgct gcggcagtgg
    29761 gcgatgcggc gcttcgggtg aggtccgcga tgcccatctt ccccctctcc atccacgacc
    29821 tgcgcttcgc gcccaacggg cgggaggtgc tcgcgggcgt ggatctggaa ctgagcgagg
    29881 agggcatcac cctggtgctc ggccccaacg gcgcaggcaa gagcgtgctg ctgcgcacga
    29941 tctgcgggct gatcgagccg agctcgggca gcatggcctg gggcggggcc ggcggtacgc
    30001 ggccgcagca gggcgtgatg atggtcttcc agcgcccgat gatgctgcgc atggccgtgc
    30061 tcgacaacgt cgtcctcggg ctcaagccgc tcggcgtggc gggggcggag cggcgccggc
    30121 gcggcctgga gatgctggag cgggtcggcc tcgccgaccg cgccggcgac agtgcgcgcc
    30181 agctctccgg cggcgagcag cagcgtctcg cgctcgcgcg cgcctggctg accaggccgc
    30241 gcctgctgct gctcgacgag cccaccgcca gcctcgaccc ctcggcgagc gcggcggtcg
    30301 agcgcatcat ccgcgagatc cgcaccgacg gcacgcgcat cctcatggtc actcacaacc
    30361 tcggtcaggc gactcgcctg ggcgacgaca tcgtcttcat ggcgggcggc cgggtgtgcg
    30421 agcacacccc ggtgcgcgcc ttcttcgccc gtccgcgctc ggaggaggcg cgcctcttca
    30481 tccagggaga attgccatgg cggatgcgtt ttcagggagc atgagatcga cgctgcggga
    30541 ggcggcgtga tgagtccggt gcccgcccgt ctgccgctcg ccgcactcgt gccgttcgtg
    30601 ctcgcggccc tggcgttcgt ggccgcgctg ctgtgcttcg ccggtgccgg cgcgctcctg
    30661 caccccgcct tgtccgaccc gatggccggg ctggccttcc tcgtctcggc gatcgcgctc
    30721 gccggcaccg gcgccttccc cttggtcttc gcgcgtctcg cgcggcgcga tgcaggcccc
    30781 cagccgccgc ccggcgcctg aggcctcgct ccttacgccc gacgccgcgc ccccctcacg
    30841 ccttccagtg ccccgacttg ccgcccagct tctccatcag ctggatgccc tccatgcgca
    30901 tgccgcggtc caccgccttg cacatgtcgt agatggtgag caggccgacg ttgaccgcgg
    30961 tgagcgcctc catctcgacg ccggtgcggc cgacggtctc ggcggtgacc gtgcagcgga
    31021 tggcgtgcgc ggccgcgtcg agctcgaact cgaccgcgac gcgggtgagc gggatggggt
    31081 ggcagagcgg gatcagctcg ctggtgcgct tggaggcctg gatcgcggcg atgcgggcga
    31141 tgccgagcac gtcgcccttc ttcgcgctgc ccgacttcac catgtcgaag gtggcgggca
    31201 gcatgtggat gcggccggtg gcgaccgcga cgcggcgggt ttcggccttg gcgccgacgt
    31261 cgaccatgtg ggcctggccg tcggcgtcga agtgggtcag cggactggat tccggagtgc
    31321 tcatggtggg gcagggcaga agatgcgggt gtgccgcggc ggcaatcgcc acgcggcggg
    31381 aggcgccgct acacgtcgat acatcggcgg gggaacgggc gcggatcggc gggtatcata
    31441 gcatccgatg atgcgccgac ccctcgccct gctgctcagc ctcgcgctgg ccctgcctgc
    31501 gccggccccc gtgctcgccg ccgatctgcc cgatctcggc gacgtggccg cgtccgaact
    31561 ctctcccgcg gccgaacgca agatcggcga acagatcatc cgcgagatcc gctggcgcga
    31621 cgcggcctat ctcgacgatg cggaggtcga ggagtacgtg aaccgcctcg gccagcgcct
    31681 cgccgcggtg agcaacaacc cgggactcga cttcgacttc ttcgtcgtgc gcgacgccac
    31741 gctcaacgcc ttcgcgctgc cgggcggctt catcggcgtg cacaccgggc tgatcctggc
    31801 ggcggagggc gagtccgagt tcgcctcggt gctcggccac gagatcgccc acgtcaccca
    31861 gcgccacatc gcgcagatcg tcggcaagca gagccagtcg gcgatgctga tgatcgcctc
    31921 gatgctggtc gcggtgctgg ccgcgcgcag caactcggat gtgagcaccg cggcgatcgc
    31981 tgccggccag gccggggcga tccagtccca actcggctac acccgcgcct tcgagcgcga
    32041 ggccgaccgc gccggcctcg agaccctgga caaggccggg ctcgacgtgc gcggcatgcc
    32101 cggcttcttc gagcgcctgc aacgcaacac gcgtgtgtac gagaacaacg cgccggctta
    32161 cctgcgcacc cacccgctga ccaccgagcg catcgccgac atggagaacc gcgtcgcggc
    32221 gatgcgctat cgccaggtgc cggactcggc ggatttccgc ttcgcgcgtg ccaagctgcg
    32281 cgccaacgcc ggccagcccg ccgaggcggt gcaggagctg gaggaccgcc tcgcgcgcga
    32341 gcccaaggac gaggcgctcg cctacggtct cgcccgtgcg ctgatgcgcg ccggtcgcct
    32401 cgacgaggcc gaggcccgtc tgcagccgct gcgcgcgaag gcggcgggcc tggcctgggt
    32461 cgaggggctg gcggcggaga tccgtctcgc gcgcaacgac gccgggggcg cgatccgcat
    32521 cctcgaggcc gcgcagaagc agtttccgtc cagccgcagc gtgagctacg cgctcgccga
    32581 tgcgcgcatc ctcggcggac gggcggacgt cgccgcgacc gaactgcgcc gccgcatcga
    32641 caaccgcagc ggtgatccgc gcctgtggca gctgctgtcg cgcgcctacg cggccctcgg
    32701 acagcgcacc gagcagcacc gcacccaggc cgaggtgtac tacctgcgcg gcagcctgcc
    32761 ggccgcgatc gagcagcttg agatcgcgcg cagggccagc gatggcgact tctataccct
    32821 gtcggcggtg gatgcgcgcc tgcgcgagct caagcagcgc ctgctcgagg aaaagcgcga
    32881 gcgctgaacg acctcgcgcc gcatgtcgcg cagcgtgacg ggcgcgcctt ccggggcgca
    32941 atgccatcct ttagaatcgg ccgccttcaa cacgacccaa ccagggagcg gggatgagat
    33001 acgacaagga agtggatgcg cgcgggctca actgcccgct accgatcctg cgggccaaga
    33061 agatgctgtc cgagatgcag agcggacaga tcgtccgcgt gctggcgacc gatcccgggt
    33121 cgatcaagga tttccaggcg tttgcgcgcc agaccggcaa cgagttgctc ggccagggtg
    33181 aaacgtccga caaggcctac gagttcttcc tgaagcggaa gtagttttcg gcgcttgccg
    33241 gagttccgcg cgggctgggg cgccgcatcg cgactcgcgt cgctcccacg ggcgctttcg
    33301 gtaggagcga cgcaagtcgc gatt
//
LOCUS       DAOQBS010000020        32971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_741_length_32971_cov_17.170950, whole genome shotgun sequence.
ACCESSION   DAOQBS010000020 DAOQBS010000000
VERSION     DAOQBS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 32971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32971
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_741_length_32971_cov_17.170950"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /gene="actP"
                     /locus_tag="PKN25_04135"
     CDS             <1..291
                     /gene="actP"
                     /locus_tag="PKN25_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764712.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNV90118.1"
                     /translation="CTTRGATIGGFLGLITAVVLTVVSPSVWEVTLGNPKGSALFPYA
                     SPALFSMAAGFIGIWLFSILDNSARAKADRAGYMAQKVRSETGIGAASASAH"
     gene            complement(367..1809)
                     /locus_tag="PKN25_04140"
     CDS             complement(367..1809)
                     /locus_tag="PKN25_04140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNV90119.1"
                     /translation="MQTSLRRKLLSSLAAPTGLLLAIAGAVAYGTAKGMVGDAYDHNL
                     LNLAHAIATHVHPEADGLNLRLSSEVEAVLRSDTQDRIYFRVRDLGGRLLGGDAALPL
                     LDDSDAASALPVPYSPVGRETPQPPRAPSTRPLFRDLVHAGEPIRALRLYRETPAGGY
                     YVTVAETLVKREAAMDRLLLGFVSAGALLLLAAGIAAGLGIPSGLAPLERLEESLRRR
                     AGADLSPIDPKAVPDEVREVVRALNGLLERQRNASARQREFLQDAAHQLRTPLAGLQM
                     QLELLESRPLDEAARHRLRVSVARVIRLANQLLALARAEAGGRLVADASEVELTALID
                     PLVEDWIERADARAIDLGVEREAVRMVGDPTLLQELIANLMDNALKYCDEGGKVSLRC
                     AREEGQVCIEVDDDGPGIPREVREQVFERFYRHAGTRASGSGLGLSIAREIVRGHGGS
                     IAIDDGPDGRGTRVRVMLPIDGHHGKTAGR"
     gene            complement(1787..2464)
                     /locus_tag="PKN25_04145"
     CDS             complement(1787..2464)
                     /locus_tag="PKN25_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV90120.1"
                     /translation="MRLLLVEDDPALAEGIAEFLRGQGDEVEVEGDGMRADRLLQNNG
                     YDFVLLDVGLPGLGGYELVRRIRKRGQRMPVIVITARDALDDRIYGLDLGADDYLVKP
                     FELAELSARMRAVQRRGGGAGARLAFGALALDLDGRLAELDGAPMTLSGREWEVLEAL
                     VRADGRTVTRENLQGDGSGNALEVCISRLRPRVEAAGLLIRTVRGFGYRLERSKEQRD
                     GADQPPA"
     gene            2639..3901
                     /locus_tag="PKN25_04150"
     CDS             2639..3901
                     /locus_tag="PKN25_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNV90121.1"
                     /translation="MSDMLPLDAEQIEAFLIATGIGLLIGLERERVPSARAGLRTFGL
                     VGMLGALVSMLGEQLGSPAPFVAGLVLVGLTIIAAYLRHPDSSDPGTTSVAALVLCYC
                     LGAASWLGYAQLAVMLAVGATVLLYFKAQLRGVATRLEQRDWISILQFGVLSLVILPM
                     LPDEEMGPHGALNPRQIWWMVVLIAGVGLAGYAALKIAGVRYGAAFVGVFGGLASSTA
                     TTLLYARQARELPAAGAMAALVIVIANLVMVLRVWGIAAVVAPGVAAPLLKLIVPALV
                     LGTLGALWLWRRLAERHETVLPATGNPTELKTALGFGAAYALVLLAAAWLSDYAGNRG
                     LYVLAFVSGLTDVDAITLSSLRLYALERLELVATVTALGIAMLANLCFKLGLASVIGG
                     RALALRAAAGMASVALGLLAGLAWVGLG"
     gene            complement(3936..4103)
                     /locus_tag="PKN25_04155"
     CDS             complement(3936..4103)
                     /locus_tag="PKN25_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90122.1"
                     /translation="MDKERLFRTGFAAFWLIALGLLVLDTMSSSRIRVEPPPIALGSG
                     QAASGGHCSGR"
     gene            complement(4114..5286)
                     /locus_tag="PKN25_04160"
     CDS             complement(4114..5286)
                     /locus_tag="PKN25_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNV90123.1"
                     /translation="MRWFFRFLLVFGALAGVLAVTLAAGLRQGLLALLGVGFGAVLQG
                     ARFGFTTGWRDMIERRDPQGLWAQMLLMVLAAALTLPLLAGSGGELVGAVAPLTISLV
                     LGAFLFGAAMQLADGCGSGTLYKAGGGSPLSLVVLPTFAFGSFLGASHQPAWIGLGGL
                     PAVNLLAFGWPTALALTVAGCGLVAWLAGRAARRAGGDAPPSPQAIAERLVRTGTASF
                     VAAAGPAQSRWATRWWLGALLLAVLYLAHLVVAGQPWGIVYGMGVWGAKAVSTLGWDL
                     SGDAFWGVAPHAQRLVEPILADVTSVTNIGLIYGAIAASRWNGAPKFSAPSGRRLAAA
                     AVAGLVMGYSARMAFGCNVGAYLGGIASASLHGWVWFALAFAGSILGVRIRRRVGA"
     gene            complement(5300..6295)
                     /locus_tag="PKN25_04165"
     CDS             complement(5300..6295)
                     /locus_tag="PKN25_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV90124.1"
                     /translation="MLKKLAPLPLTIALAASLGFATPAFASTTGQSPAAAVATTAAPA
                     PLLQPAELKTLLGQPGVRVLDIRAEKDYAAGHVPGAVNTPYGKYRGPKENPGQLPAEA
                     ALTQLLQAAGVDRDTHVVVVHAGSDHTDFGAAARVYWTLKVGGLTRLSILDGGSKAWQ
                     AAGGALETAAPQVAPSQFSYRYDQEQVVTSEELAAAVKAGNAPLLVDARPPKFFAGET
                     RTDAAARYGTLPGAKSFDNAQFFPQGGTTLKPASELKALVAQSGAAAAPAVSFCNTGH
                     WAATNWFVMSEIAGNKAVKLYPSSVVEWSQSELPMDNQPSRLSALVQDAKRALDK"
     gene            6294..7316
                     /locus_tag="PKN25_04170"
     CDS             6294..7316
                     /locus_tag="PKN25_04170"
                     /inference="COORDINATES: protein motif:HMM:NF023984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HNV90125.1"
                     /translation="MVVSSVGSVCWGPARRTARRCHDAPQNTGKLKYSKRLSKKKFIT
                     FQNENDGCAGVELDARAAIEAQLALRPRNRKNRLNTLSIGLRILAVLLLLAVGLSFAN
                     DPRPAASGAEGGWRGAARHSAGIAPDPERFADTAIVQVYAAATYGWRGYFAVHPWIIY
                     KRAGDTVYTRYDVIGWGRGNVVRRNYALPDGLWFGAVPRVLVEHRGEGVEAMIGQIEA
                     AVASYPHADEYRSYPGPNSNTFLAHIGREVPALRLDLPANAIGKDYRPVTRPLGVSPS
                     GSGVQFSLLGLLGLSVGVQEGIELNVLGLNFGLDLNRPGLRLPAIGRLGLDDASVSER
                     GAAGLQ"
     gene            complement(7398..8321)
                     /locus_tag="PKN25_04175"
     CDS             complement(7398..8321)
                     /locus_tag="PKN25_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV90126.1"
                     /translation="MSHTPQPARWLPFVVLGIGLAAISFGAIFARLAQAEGVSSLAVA
                     TWRLGFAALIITPVAFLQSRHELARLTRRQIGMALAAGFFLALHFATWISSLEYTSVA
                     SSTALVTTNPLWIGLASFLIFRETPTKMMIGGIALSFAGSLFIFWSDSQTAGAGSNPM
                     FGNLLALVGSWCFSAYLLIGRRLRAGLGLPAYIWLAYGAAALFLFAASGAGGVELFSL
                     SNTAWLVLLAMALGPQLLGHTAYNWSLRYVSATFVAVVTLGEPVGSALMAFLVFGEGF
                     APLQFVGFSLLLVGIYLAAKGERAEKGAGSD"
     gene            complement(8666..9379)
                     /locus_tag="PKN25_04180"
     CDS             complement(8666..9379)
                     /locus_tag="PKN25_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90127.1"
                     /translation="MLKMEGVHAHYGHIHALRGIDVEVQVGEIVTLIGANGAGKSTLM
                     MSLFGNPKASAGRIVFDGEDITRVHTHEIARRGIALVPEGRRIFPRMTVIENLQMGTA
                     HADPANYAADTARVFAMFPILQERQAQRAGTLSGGEQQMLAIGRALMSRPKLLLLDEP
                     SLGLAPIYIRKIFQIVRELNRDHGMTILLVEQNANQALRVADRGYVLQHGEITLAGTG
                     AELLASPEVRAAYLEGGHA"
     gene            complement(9379..10212)
                     /locus_tag="PKN25_04185"
     CDS             complement(9379..10212)
                     /locus_tag="PKN25_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV90128.1"
                     /translation="MNTPATLLSVRNLTMRFGGLTAIDDLSLEVPAGKITGIIGPNGA
                     GKTTLFNCLTGFYKPTTGTLRLAHPTRGEMRLESLPSHRVARDAQVVRTFQNIRLFPK
                     MTVLENLIVAQHNVLQRASKFSIAGIFKLPGYRQAERQAIERAAMWLERLKLTHLADD
                     AAGDLPYGIQRRIEIARALCVDPVLLCLDEPAAGLNPRESAELNELLLYLCGEMGIAI
                     LLIEHDMSVVMNVSDHIGVISYGRKIAEGTPAQVKHDPQVIKAYLGEDEEEEVTGGAA
                     A"
     gene            complement(10209..11540)
                     /gene="livM"
                     /locus_tag="PKN25_04190"
     CDS             complement(10209..11540)
                     /gene="livM"
                     /locus_tag="PKN25_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HNV90129.1"
                     /translation="MSAVVFARHAMTPAERLRDAGWVAFVGLILGIPLVGLTTVDVGG
                     RLGVETRFGLLAAAVAAVFAGRLLLRWAIDQARNRKQERDRASTATAADRAHGAQRVV
                     NVLGWVALGASIALPIVFSDNRYVVDTATTVLIYVMLGWGLNVVVGLAGLLDLGYVAF
                     YAVGAYSYALLSTQFGWSFWEALPVSGALAASFGILLGYPILRLRGDYLAIVTLGFGE
                     IIRIILVNWTEFSNGPNGISSIPRPSFFGLDFTRSAPEGGQTFAGFFGLEFSPMHRLI
                     FLYYLILVLALLTHAFVSRLRKLPVGRAWEALREDEIACQAMGMNTTNIKLSAFAIGA
                     MLGGFAGVFFAARQGFISPESFTFTESAIILAIVVLGGMGSQMGVVLAATLLVLIPEF
                     GRNFAEYRMLLFGLAMVLIMVWRPGGLLSHREPTVHLNASGKTAATKEAAR"
     gene            complement(11540..12454)
                     /locus_tag="PKN25_04195"
     CDS             complement(11540..12454)
                     /locus_tag="PKN25_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV90130.1"
                     /translation="MAYALQQLINGLTLGAMYGLIAIGYTMVYGIIGMINFAHGDIFM
                     VSAFIAVTAFTILAAADVTSIPLALTFVLIVSIFFTSAYGWAVERTAYRPLRGSTRLA
                     PLISAIGMSIFLQNSVQLTQGARVKPIPPVLEGGIVLWEAPGFTVQIGWSQMLIIVTT
                     VILMSAFTWMITQTPFGRQQRACEQDRTMAALLGVNVDRTISYTFMLGAALAAVAGVM
                     VTVYYGVVDFFIGFLAGIKAFTAAVLGGIGSLPGAMLGGLLIGLIESFWAAYLWAEYK
                     DVATFGILCLVLMLRPSGLLGRPEVEKV"
     gene            complement(12664..13758)
                     /locus_tag="PKN25_04200"
     CDS             complement(12664..13758)
                     /locus_tag="PKN25_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV90131.1"
                     /translation="MKKTLLATAVLALGGFSNAALADLAVAIAGPMTGQYASAGDQIR
                     KGAEMAIADINARGGVLGQKLKLEVGDDACDPKQAVSVANTMVNKGIVFMHGHWCSSS
                     TIPASEVYVEADIPMATVSTNPQVTERGLKNIFRIMGRDDQQGLVAGGYIADHFKGKK
                     VAVVDDKSAYGKGLADEMAKAMEAKGAKPALRESITAGEKDYSGIVTKLKQAGVEVMA
                     YGGYHTEVALILRQAQAAGLQLTVMGGDTMTNSELVTAAGPAADNVMFTFSPDARKNP
                     DAAPVVEKFRAAKTEPEGYVLYAYAAMQLFEQAATTAKSTKYADLEKAMRGGSFKTVI
                     GDLAFDAKGDQKAPGFVVYRWQGGKYDYAK"
     assembly_gap    13938..14037
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14281..18006)
                     /gene="putA"
                     /locus_tag="PKN25_04205"
     CDS             complement(14281..18006)
                     /gene="putA"
                     /locus_tag="PKN25_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HNV90132.1"
                     /translation="MTDNIPSSARAAAALDAAAALPPTARSTVRHAIDAAWRTPEPEC
                     VPALVEAARLPAALREPVRALAHDLVVGLRATRTRSSGVDALMKEFSLSSQEGVALMC
                     LAEALLRVPDKATADRLIRDKLADGDWRAHIGNSPSLFVNAATWGLLITGRLVSTSSE
                     QGLSSALTRLVARGGEPLIRKGMDLAMRMLGEQFVTGRDIDEALERGREHENKGYRYS
                     FDMLGEAAMTASDAERYFRDYERAIQAIGVASRGRGVVDGNGISVKLSALHPRYTWSQ
                     RERVLAELLPKLKTLCLQARRHDIGLNIDAEEADRLDLSLDLLEALATDDALAGWTGL
                     GFVVQAYQKRAPHVLDFVIDLARRSGQRMMVRLVKGAYWDAEIKRAQIDGLAGYPVYS
                     RKVYTDVSYLACAKKLLAARDVIYPQFATHNAHSLAAIFHLAAADGRAWQPGDYEFQC
                     LHGMGEPLYDQIVGRADRHRLVRIYAPVGSHETLLAYLVRRLLENGANTSFVNRIVDE
                     RVPVEELIADPVEQAAPLAGAPHPKIALPAELFGGLRTNSAGIDLASEPVRARIAAAL
                     ANSRTTPFAAGPIVAGRSAVPADVTPVSNPADRGELVGGVAEATTDDLEHALAAASAA
                     AADWAATPAAARSERLERAAALFEREADTLLALAVREAGKSWANAVAELREAVDFLRY
                     YAQQARGFDPASHVALGPVLCISPWNFPLAIFSGQVAAALAAGNPVLAKPAEQTPLIA
                     AHAVRLLHEAGIPAAALQLLPGRGETVGAALVADARVRGVMFTGSTEVAALINRSLAA
                     RGGKVPLIAETGGQNAMIVDSTALPEQVVTDVLASAFDSAGQRCSALRVLCLQQDIAD
                     DVLHMLQGALAELRLGNPADVRVDIGPVIDAGARDGLEAHVAAMLAKGARITRLPLPA
                     ACAHGTFVAPSIIEIGALSELGREQFGPILHVLRYRASELDSLIDAIDASGYGLTMGV
                     HTRIDETVERVAARAHVGNLYVNRNIIGAVVGVQPFGGEGLSGTGPKAGGPLYLHRLL
                     ARTPGPVLADGALAVTDSDETRAADAAFSGFLEWLDGAGRGLLEGDELAALRDRADAC
                     RARRIAGLRLALPGPTGEDDSLRFTARGTIAGVADSAAAVLHQVMAALASGNRLLLAD
                     RAAARTVQAALPEALRAYVAVDAAWFDKALGAVLFDGSDAEADALQVRVAGRSGPILQ
                     LLQPCPDYDLGRLVHERTLSINTAAAGGNASLMAMGA"
     gene            18202..18666
                     /locus_tag="PKN25_04210"
     CDS             18202..18666
                     /locus_tag="PKN25_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNV90133.1"
                     /translation="MDRIDQKILQELQADARLSIVELSRRVGLTKTPCAERIRRLEKS
                     GVIRGYHAALDPDAVGAGHIVVVQVLLTSTTEQDLRRFNEAVRRIPEIESCLMIAGDF
                     DYLLKVRTRDINEYRRVMGEQISGLPCVKQTHTYVVMEVVKDERKLPLRGSA"
     gene            18825..20687
                     /locus_tag="PKN25_04215"
     CDS             18825..20687
                     /locus_tag="PKN25_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HNV90134.1"
                     /translation="MDSKRAMPSLTIGAIGVVFGDIGTSPLYALREIFNGHHPIPVTP
                     GNILGILSLVLWAIMVLVTLKYVLILMRADNRGEGGSLALLSLVVTHARSARLAWVVS
                     MLGIFAAALFFGDSMITPAISVLSAVEGIGIVAPGLEAWVVPVTVLILTVLFAIQRRG
                     TGVVGLFFGPVMVVWFAVLGALGMVEIVRYPEILAALSPLHAVAFVVDHPALAFLALG
                     AVVLAVTGGEALYTDMGHFGPAPIRLGWFGFVLPALVVNYFGQGALLLHEPAAIESPF
                     YHLGPSWAMIPLLVLATLATVIASQAVISGAFSVARQAVQMGLLPRMLIVHTSGHEQG
                     QIYVPFTNWTLYLAVVALVVGFQNSSNLAAAYGIAVTGTMLIDTVLLGFVMVLIWRWR
                     PLLAGLCAGTLLLVDLAFFSANAIKIPQGGWFPIAMALVSFTVLTTWRQGRRLVQQEI
                     AAQGLPLNAFIDMVGEVHRVQGTAVFMTSTKDGVPAALLHNLKHNQVLHERVALVTVE
                     TADTPHVSELDRIYLHRIRNGFMRVIVRYGFMEDPDIPSALKHCSRFGESFEMMETTF
                     FVSRETVIPRLSRRLFSDWRARLFAFMSKNATSATDFFRIPNNRVVELGTQLVI"
     gene            20807..22120
                     /gene="gshA"
                     /locus_tag="PKN25_04220"
     CDS             20807..22120
                     /gene="gshA"
                     /locus_tag="PKN25_04220"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNV90135.1"
                     /translation="MVPHLTTALTGPLLELERQFLDNACDIEKWFRTQWQDHMPPFYG
                     STDLRNSGFKLAPVDLNLFPGGFNNLNDVFMPLCVQAAQAAIERICPDASKLLLIPEN
                     HTRNQFYLQNVAKLASILRLTGLDVRIGSLLPEIAAPTVLELANGATLTLEPLRRSGS
                     RIGLDDFDPCAVLLNNDLSAGIPQVLKGLDEQWLIPPLHAGWHKRRKSKHAECYDRVA
                     REFAAAIGIDPWRINPEFGVCGQINFQERTGEECLAAQVDSLLTRIRAKYKEYGVTDE
                     PFVVVKADAGTYGMGVMTVKDASEVVGLNRRQRNKMAVVKEGLQVHEVIIQEGVHTFE
                     TVEDGVAEPVVYMMDHYVVGGFYRVHTERGRDENLNAPGMHFKPLAFDTSGTMPDAAQ
                     GPDAPPNRFYAYGVVARLALLAASVEIEETAPAEEEDAGGDALAA"
     gene            22170..23126
                     /gene="gshB"
                     /locus_tag="PKN25_04225"
     CDS             22170..23126
                     /gene="gshB"
                     /locus_tag="PKN25_04225"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNV90136.1"
                     /translation="MTRKLKLLFILDPLEGLKAYKDSSIAMMRAAARRGHEVWALQRA
                     GLTWREGVVVGTGCVALAVCDDDAAWYRAGEAQALPLAAFDAVLMRQDPPFDFEYVTA
                     TWLLERAAQAGVRVFNDPRAVRDHSEKIAITEFPQFTATTLVARDPADIHAFIDELGD
                     VILKPLDGMGGSQIFRVRADDPNRNVIVETLAHDGTRTIMAQRYLPQIVDGDKRVLII
                     GGEVVPFALARIPRAGETRGNLAAGGRGVAMALTAREREIAGFLAPTLWARGLLIVGL
                     DVIGGHLTEINVTSPTCMVEIAAQQGFDVAGLVIDKLERACG"
     gene            23117..24151
                     /locus_tag="PKN25_04230"
     CDS             23117..24151
                     /locus_tag="PKN25_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNV90137.1"
                     /translation="MRLNLRILALAACALLLAACSRAQVFHHEAYVFGTRVDVAVYGE
                     NQAEADRAAAAVLREFDRLHRAYHAWEPSELTALNAAIARGEAATVSDELAAMLKDAQ
                     AIAATGDGLFDPALGGLVALWGFHTDTFVPQRPDPAKLQALLAAHPSMADLVLDGNRV
                     ESRNPAVQLDLGGYAKGYALDRAAAILRAQGVADALINIGGNVMALGAKGDQPWRIGI
                     QHPRAPAPLATLPLYDGEAIGTSGDYQRYFELDGERYAHLLDPRTGQPAHGTQSLTVL
                     ITPRANAGTLSDAPSKPAYLAGDGWREQIRRFGIEHALRVAADGRVEVTRALRARLQF
                     PSPVEATVVD"
     gene            24182..24889
                     /locus_tag="PKN25_04235"
     CDS             24182..24889
                     /locus_tag="PKN25_04235"
                     /inference="COORDINATES: protein motif:HMM:NF018049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HNV90138.1"
                     /translation="MTPQTAWLLAISAVVIVGVIAFFVGRSSAGTKGRIDELEAELAR
                     KAEEAERYRKEVDAHFDKTATLFVSMAGSYKELFEHLSAGYDKLSGASARERFQERVD
                     ALLPRRVVEPAAAGALLAGGAAGAVAGADAAEPTGSSADAVVGPAPVSVVDAAPVPEA
                     EDLAESVPPSVGATSGVSADEAEKAAAESVAAPAGAEEPGSAGADADAAERVRRAEGA
                     AAETVGESGAESRGETR"
     gene            24954..25841
                     /locus_tag="PKN25_04240"
     CDS             24954..25841
                     /locus_tag="PKN25_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HNV90139.1"
                     /translation="MSARALHRLFLIACSALLLVGCGLRFAYSQLDWLLPWYLRDYVT
                     LDAGQRGEFDRRLAGLLDWHCRSHLPEYVALLRAANATLAAERVEPAQLERFLERGEA
                     LWREIVGELEPELRRLAAGLGDEQVEELAAAFVRRGEEARAEFLSGDESAQHAARVER
                     MEERLRRWFGRMTPAQRERIAAWSRALQPTTEAWLEDRARWQAELLDALRVRADAAAF
                     APRLAQALAPREARWSAQHRAAVAHNRARTLELLAELHALSSAAQRRQLRDEIDMLAT
                     QFAGISCAEPARVSAAGGR"
     gene            25891..26772
                     /locus_tag="PKN25_04245"
     CDS             25891..26772
                     /locus_tag="PKN25_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV90140.1"
                     /translation="MHKTELDRLAIGLMVVLCSIWGLQQVAIKLASAGISPVWQAGLR
                     SIGATAIVVVWALLRGVKLFERDRSLAPGLLAGALFAGEFAMIFLGLQHTSASRGVIF
                     LYTAPFFVALGAVWLLPQEHMRRRQWMGMALAFVGVLALFGENLFAATEGAWVGDLML
                     LGAALLWAATTLTVKASALARASAEKTLLYQLGVSALVLPPLSLAMGEPGVFAPTPMV
                     WANLFFQAAIVAGVSYLAWFWLVREYPATRLSSFSFLTPVMGVLAGGLILGEPLTPAV
                     YAALFLVGSGIWVANRS"
     gene            26897..27505
                     /locus_tag="PKN25_04250"
     CDS             26897..27505
                     /locus_tag="PKN25_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HNV90141.1"
                     /translation="MSRPVVEFWFEFASTYSYLSVMRIERAAAATGVDIEWKPFLLGP
                     VFLALGWNDSPFNIYPPKGRYMWRDLERLAEKEGLPFRHPSRFPRNGLLAARVALVGV
                     EEGWVAPFARAVMSANFAEDREIGEEAVIVEILGALGLPAAELMARAQADANKLALRC
                     QTERAAELGLFGAPSFRVGEELFWGNDRLEDALAWACRADRR"
     assembly_gap    27814..27913
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    28070..28169
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28196..28930)
                     /locus_tag="PKN25_04255"
     CDS             complement(28196..28930)
                     /locus_tag="PKN25_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90142.1"
                     /translation="MSSIAPLGHPNPETARMIRIRSLSLVVAHRSIMPGLCAALLAST
                     ILPAHAQTADEAIRAAERTRAEALAADGKALRAEAEATYQATVPGCYEKFLVNRCIDQ
                     AKKQRLETIRRARELEAESRGITLAERQRAAAAKPAAGERPSPPAPPAAPPAASPLVP
                     SGPSGDATIAPSTEAERIRAQRVEASREAEAATARQRAAQDAERASARRDAEDAAARR
                     ARQAEEDRARYEKRLREYEQEQAGKK"
     gene            29057..30988
                     /locus_tag="PKN25_04260"
     CDS             29057..30988
                     /locus_tag="PKN25_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV90143.1"
                     /translation="MISLRNLRLARGAKVLIESATLQIHPGWKVGLTGANGSGKSSLF
                     AMLRDQLHPDQGDLDLPPGWVIAHVAQETPGLAQPAIDYVLDGDAELRRIEAELAAAE
                     AAHDGSHIGELHARLHEIGGYAARARAAALLDGLGFLQSDIERPVADFSGGWRMRLNL
                     AQALMCRSDLLLLDEPTNHLDLDAVIWLEAWLRDYRGTLLLISHDREFLDACVTHIAH
                     IEQQKLGLYTGGYSDFERQRGERLIQQQAMFERQQRDIAHMEDYIRRFRAKATKARQA
                     QSRIKALERMERIAAAHVDTPFSFAFRDAPPAPDPLLQIEDGAVGYGDKTILERITLT
                     LRPGERVGLLGRNGAGKSTLIKLLAHELQLASGRRLEGKGLATGYFAQHQLETLRPDE
                     SALQHMVRLDPQTREQELRDYLGGFDFRGDGVGGTSTPATTPCGPFSGGEKSRLALAL
                     LIWQRPNLLLLDEPTNHLDLEMRHALTMALQDYEGAMVLVSHDRALLRATCDRFVLVD
                     GGRIQPFDGDLDDYRDWLASRRAEVAAAAACPDQAADKAARKADRAQAAADRQARLVA
                     RRPLVKELEQIDKRMAAWNKEKAELDAKLADPALYTGPQAGEVPALNKRQAELAERIE
                     EAELRWLELHEALEAIPAD"
     gene            complement(31049..32251)
                     /locus_tag="PKN25_04265"
     CDS             complement(31049..32251)
                     /locus_tag="PKN25_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNV90144.1"
                     /translation="MIKLIPIEKLQPGMYVHDLNCSWMEHNFVRSRFAVKDEAVVRKV
                     RAIGTHEIYIDTARGLDLPEAPTREEIEHEVDEKIDAIAAEAAPRIAAQEVACEIERA
                     RALHREANLIVRDMMADVRLGKQIEMELIEPLVERIVDSIFTQQDALIPLARLKQHDA
                     YTFQHSVSVCALMTTFARNLQLPRPVIHEIAAGALLHDVGKASVPDAILNKPAKLTDA
                     EFAKMKNHVVQSKIILQATPGISAIALDVAAQHHERFDGTGYPNKLAGDAISLYGQMG
                     AIVDVYDAITSNRVYHKGMPPTEALRKLLDWSKFHFKPELVHAFIRAVGIYPAGSLVR
                     LESKRLAVVQAQNPDKLIAPKVTVMFHTDGYALTPQDLDLRHAQDRIVGHEDFATWKL
                     NPAHWLPK"
     gene            32454..>32971
                     /gene="thrH"
                     /locus_tag="PKN25_04270"
     CDS             32454..>32971
                     /gene="thrH"
                     /locus_tag="PKN25_04270"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNV90145.1"
                     /translation="MRIVCLDLEGVLVPEIWIEFAERTGIPELRRTTRDEPNYDTLMR
                     YRLDILAQKKLGLPDIQDVIASMGPMPGAREFLDELRDAYQVVILSDTFYEFAKPLMK
                     QLGMPTLFCHSLEADAGGILVDYHLRMPDQKREAVRRFKELNFKVAAAGDSYNDTAML
                     GEAHGGILFHPP"
BASE COUNT         4649 a        11176 c        11765 g         5081 t
ORIGIN      
        1 tgcacgaccc gcggcgcgac gatcggcggc ttcctcggcc tgatcaccgc ggtggtgctg
       61 accgtcgtgt cgccgtcggt gtgggaagtc acgctcggca acccgaaggg ttcggcgctg
      121 ttcccctacg cttcgccggc cctgttctcg atggccgcgg gcttcatcgg catctggctg
      181 ttctcgatcc tcgacaacag cgcgcgtgcc aaggccgacc gtgccggcta catggcgcag
      241 aaggtgcgtt cggagaccgg tatcggcgcg gcgtcggcct cggcgcacta agcagcaagc
      301 gctccgccgg catccgcgag ggtgccggcg ggcagcacgc aagaccgcgg ggcggtgggc
      361 gcaggctcac cgccccgcgg tttttccgtg atggccgtcg atcggcagca ttacgcgcac
      421 gcgggtgccg cgcccgtccg ggccgtcgtc gatcgcgatg ctgccgccgt ggccacgcac
      481 gatctcgcgc gcgatcgaca gacccaggcc gctgccgctg gcgcgggtgc cggcgtggcg
      541 gtagaagcgc tcgaagacct gttcgcgcac ttcgcggggg atgcccggac cgtcgtcgtc
      601 gacctcgatg cacacctggc cctcttcgcg cgcgcagcgc agcgagacct tgccgccttc
      661 gtcgcagtac ttgagggcgt tgtccatcag gttggcgatc agctcctgca gaagggtggg
      721 gtcgccgacc atgcgcaccg cctcgcgctc gacgccgaga tcgatcgcgc gggcgtcggc
      781 acgctcgatc cagtcctcga ccagggggtc gatcagcgcg gtcagctcca cttccgacgc
      841 atccgcgacc aggcggccgc ctgcttctgc gcgggcaagc gcgagcaact ggttggcgag
      901 ccggatcacc cgcgccaccg agacgcgaag gcggtgccgc gcggcctcgt cgagcgggcg
      961 cgactcgagc agctcgagct gcatctgcag gccggcgagc ggggtgcgca gctggtgcgc
     1021 ggcatcctgc aggaactcgc gctggcgggc gctggcattg cgctggcgct cgagcaggcc
     1081 gttgagtgcg cgcaccacct cgcgcacctc gtcgggaacg gccttggggt cgatcggcga
     1141 caggtcggcg cccgcgcgcc ggcgcagcga ctcctccagg cgctcgagcg gggcgagacc
     1201 gctcggaatg cccagtccgg cggcgatgcc ggcggcgagc aggagcaggg cgccggcgga
     1261 gacgaagccg aggagcagtc ggtccatcgc cgcctcgcgc ttgaccaggg tctcggcgac
     1321 ggtgacgtag tagccgcccg cgggtgtttc gcgatacagg cgcaaggcgc ggatcggctc
     1381 gcccgcatgg acgagatcgc ggaacagcgg tcgcgtggag ggcgcgcgcg gcggttgcgg
     1441 tgtctcgcgg ccgaccgggg agtagggcac cggcaaagcg gatgccgcat cgctgtcgtc
     1501 gagcagcggc agggccgcgt cgccgccgag caggcgcccg cccaggtcgc gtacgcggaa
     1561 gtagatgcgg tcctgggtgt cgctgcgcag caccgcctcg acttcggacg acagcctcag
     1621 gttgagcccg tccgcttcgg ggtgcacatg ggtggcgatc gcgtgggcga ggttgagcag
     1681 gttgtggtcg taggcatcgc cgaccatgcc cttggccgtg ccgtaggcca ccgcgccggc
     1741 gatcgccagc agcaggccgg tgggtgcggc gaggctgctg aggagcttac gccggaggct
     1801 ggtctgcacc gtcgcgctgc tccttgctgc gttcgaggcg gtagccgaag ccgcgtacgg
     1861 tgcggatcag caaccctgcc gcctccacgc gcgggcgcag gcgcgagatg cacacctcga
     1921 gcgcgttgcc gctgccgtcg ccttgcaggt tctcgcgcgt caccgtgcgc ccgtcggcgc
     1981 gcaccagcgc ctccagcacc tcccactcgc gcccgctgag cgtcatcggc gcgccgtcga
     2041 gctcggcgag gcggccgtcg aggtcgaggg ccagcgcccc gaaggccagc ctcgccccgg
     2101 cgccaccccc gcgccgctgc accgcgcgca tgcgcgcact gagctcggcg agctcgaagg
     2161 gcttcaccag gtagtcgtcg gcgccgaggt cgagcccgta gatgcggtcg tcgagcgcgt
     2221 cgcgcgcggt gatcacgatc accggcatgc gctggccgcg cttgcggatg cgacgcacca
     2281 gctcgtagcc gccgagcccg ggcaggccga cgtcgagcag gacgaagtcg tagccgttgt
     2341 tctgcagcag gcggtctgca cgcatgccgt cgccttccac ctcgacctcg tcgccctggc
     2401 cgcgcaggaa ttccgcgatg ccctccgcga gcgcggggtc gtcctcgacc agcaaaagtc
     2461 tcatctgtgc ttctccatgc cgcgccgcgc gggcggaccc cgcgtccatc gcgcagccgc
     2521 gagcgtcgtg tggagcgaat cgtatcccgg cacgggcgtg cttgcatcgc cgcgcacgcc
     2581 ccggcgtatg atcgggctgt cgatcgccct ctgccggggc cgcacccggc ccgccgccat
     2641 gtcggacatg ctgccgctcg atgccgaaca gatcgaagcc ttcctgatcg cgaccggaat
     2701 cggcctgctg atcggcttgg agcgcgagcg tgtgccctcg gcgcgtgccg ggctgcgcac
     2761 cttcggcctg gtcggcatgc tcggcgcgct ggtgtccatg ctcggcgaac agctcggcag
     2821 ccccgcgccc ttcgtcgccg gcttggtgct ggtgggcctc accatcatcg ccgcctacct
     2881 gcgccacccc gattcttccg atcccggcac cacctcggtg gcggcgctgg tgctgtgcta
     2941 ctgcctgggc gccgcgagtt ggctcggcta cgcgcagctc gcggtgatgc ttgcggtggg
     3001 ggccaccgtg ctgctctatt tcaaggcaca actccgcggc gtggcgacgc ggctggaaca
     3061 acgcgactgg atctcgatcc tgcagttcgg cgtgctctcg ctggtcatcc tgccgatgct
     3121 gccggacgag gagatgggcc cccacggcgc gctcaatccg cgccagatct ggtggatggt
     3181 ggtgctgatc gccggtgtcg gcctggccgg ctatgcggcg ctgaaaatcg ccggcgtgcg
     3241 ctacggtgcc gccttcgtcg gtgtcttcgg cggcctggca tcgagcacgg cgaccacgct
     3301 gctgtacgcg cgtcaggcgc gcgagctgcc cgcggcgggt gcgatggccg cgctcgtgat
     3361 cgtgatcgcc aacctggtga tggtgttgcg ggtatggggg atcgccgccg tggtcgcacc
     3421 cggcgtcgcc gcgccgctgc tgaagctgat cgtgccggcg ctcgtgctcg gcacccttgg
     3481 tgcgctgtgg ctgtggcgcc ggctcgccga acgtcacgag accgtgctgc ccgccaccgg
     3541 caacccgacc gaactgaaga ccgcgctcgg tttcggtgcg gcctatgcgc tggtgctgct
     3601 ggccgcggcc tggctgtcgg actatgcggg caatcgcggc ttgtacgtgc tggccttcgt
     3661 ctccggcctc accgacgtcg atgcgatcac cctgtccagc ctgcgcctgt acgcgctcga
     3721 gcgcctcgaa ctggtcgcca cggtcaccgc gctcggcatc gccatgctcg ccaacctctg
     3781 cttcaagctc ggcctcgcca gcgtgatcgg gggccgcgcc ctcgcgctac gtgccgccgc
     3841 cggcatggcc agcgtcgcgc tcggcctgct cgccggcctg gcctgggtgg gactcgggta
     3901 aggcgcgtcc ggctttcgcg ccttaggcct tacgcctaac gtcccgagca atgccccccc
     3961 gaggcggcct ggccgctgcc gagcgcgatc ggcggcggtt ccacgcggat cctgctgctg
     4021 ctcatggtgt cgagcaccag caagccgagg gcgatcagcc agaaggccgc gaagccggtg
     4081 cggaagagtc tttccttatc catgtcttcc tcctcacgca ccgacgcggc ggcggatgcg
     4141 cacaccgagg atggagccgg caaaggcgag cgcgaaccac acccagccgt gcaggctggc
     4201 cgaggcgatg ccgcccaggt aggcgccgac attgcatccg aaggccatgc gtgcgctgta
     4261 gcccatcacc agcccggcca ccgcggcggc ggcgaggcgg cgcccggagg gcgcggagaa
     4321 cttcggcgcg ccgttccagc gcgaggcggc gatcgcgccg tagatcaggc cgatgttggt
     4381 caccgaggtg acgtcggcga ggatgggctc gaccaggcgc tgcgcatgcg gcgccacgcc
     4441 ccagaaggcg tcgcccgaga ggtcccagcc gagcgtggag accgccttcg cgccccacac
     4501 gcccatgcca tacacgatgc cccagggctg gcctgcgacg accaggtggg ccaggtagag
     4561 caccgccagc agcagcgccc ccagccacca gcgcgtcgcc cagcgcgact gcgcgggccc
     4621 ggccgcggcg acgaagctcg ccgtgccggt gcgtacgagc cgctcggcga tggcctgcgg
     4681 cgagggcggt gcgtcgccac ccgccctgcg tgcggcgcgc ccggccagcc aggccaccag
     4741 gccgcagccg gccacggtga gcgcgagtgc ggtcggccag ccgaaggcga gcaggttcac
     4801 cgccggcagg ccgccgagcc cgatccaggc gggctggtgc gaggcgccga ggaagctgcc
     4861 gaaggcgaag gtgggcagca ccacgaggga cagcggcgag ccgccgccgg ccttgtagag
     4921 cgtgcccgag ccgcagccgt cggcgagctg catcgccgca ccgaacagga aggcgccgag
     4981 caccaggctg atcgtcagcg gcgccactgc gccgaccagc tcgccgccgc tgccggcgag
     5041 cagcggcagg gtgagcgccg ctgccagcac catcagcagc atctgcgccc acaggccctg
     5101 cgggtcgcgg cgctcgatca tgtcgcgcca gccggtggtg aagccgaaac gtgcgccctg
     5161 cagtaccgcg ccgaagccaa cgcccagcag cgcgagcagg ccctggcgca ggccagccgc
     5221 cagcgtcacc gcgagcacgc ccgcgagtgc gccgaagacc agcaggaatc ggaagaacca
     5281 gcgcatcgtc gcgtccgcct tacttgtcga gcgcgcgctt ggcgtcctgc accagcgcgc
     5341 tcaggcgcga gggctggttg tccatcggca gctcggactg gctccattcc accaccgagg
     5401 aggggtagag cttgaccgcc ttgttgccgg cgatctccga catcacgaac cagttggtgg
     5461 cggcccagtg gccggtgttg cagaaggaca ccgcgggtgc ggcggcggcg ccggactgcg
     5521 cgaccagggc cttgagctcg ctcgccggct tgagcgtggt gccgccctgg gggaagaact
     5581 gcgcgttgtc gaacgacttc gcgcccggca gcgtaccgta gcgcgcggcg gcgtcggtgc
     5641 gcgtctcgcc ggcgaagaac ttgggcgggc gagcgtcgac cagcagcggt gcgttgccgg
     5701 ccttgaccgc ggcggcgagc tcctcgctgg tgacgacctg ctcctggtcg tagcggtagc
     5761 tgaactggct gggcgcgacc tgcggcgcgg cggtctcgag cgcgccgccg gcggcctgcc
     5821 aggccttgct gccgccgtcg aggatggaca ggcgggtgag gccgccgacc ttgagcgtcc
     5881 agtacacgcg cgccgcggcg ccgaagtcgg tgtggtcgct gccggcatgc acgacgacca
     5941 cgtgggtgtc gcgatcgacc ccggcggcct gcaggagctg ggtcagggcg gcctcggcgg
     6001 gcagctggcc gggattttcc ttcgggccgc gatacttgcc atagggggtg ttcaccgcgc
     6061 cgggcacgtg gccagcggca tagtcctttt cggcgcggat gtcgagcacg cgcacaccgg
     6121 gctggccgag gagggtcttc agttcggccg gctggagcag cggggcgggg gcggcggtcg
     6181 tcgccaccgc ggcggcgggg ctttgtccag tggtgctggc gaaggcgggc gtggcgaagc
     6241 cgaggctggc ggcgagcgcg atggtcaggg gaaggggggc gagtttcttc agcatggtcg
     6301 tgtcgtccgt cggttccgtt tgctgggggc cggcgcgccg gactgcgcgg cgctgccatg
     6361 atgcgccgca gaatacggga aagctgaaat actcgaaacg tctttcaaag aaaaagttta
     6421 taacttttca gaatgaaaac gacggctgcg ccggcgtaga actcgatgcg cgagcggcga
     6481 tcgaagcaca actggccttg cggccccgca acaggaaaaa ccgcttgaac accctctcga
     6541 tcggcttgcg catccttgcc gtcctgctgc tgcttgcggt ggggctcagc ttcgccaatg
     6601 atccgcgtcc cgcggcgtct ggcgcggagg gcggctggcg cggcgcggcg cgccactccg
     6661 ccggcatcgc acccgacccc gagcgcttcg cggataccgc gatcgtgcag gtgtatgccg
     6721 cggccaccta cggctggcgc ggctacttcg cggtgcatcc gtggatcatc tacaagcgtg
     6781 ccggcgacac cgtctacacc cgctacgacg tcatcggctg gggacgtgga aacgtggtgc
     6841 ggcgcaacta cgccttgccc gacgggcttt ggttcggggc cgtgccgagg gtgctggtcg
     6901 agcaccgcgg cgaaggcgtg gaggcgatga tcgggcagat cgaggccgcg gtggcgagct
     6961 acccgcacgc ggacgagtac cgcagctatc cgggcccgaa cagcaacacc ttcctcgccc
     7021 acatcggccg cgaggtgccg gcgctgcgcc tcgacctgcc ggcgaacgcg atcggcaagg
     7081 actatcgccc cgtcacgcgt ccgctgggcg tgtcgccctc ggggtcgggc gtgcagttct
     7141 cgctgctcgg cttgctgggc ttgagcgtgg gcgtgcagga aggcatcgag ctgaacgtcc
     7201 tcgggctcaa tttcggcctc gatctcaacc gtcccggcct gcgtctgccc gcgatcggcc
     7261 gcctgggcct cgacgacgcg tcagtgtccg agcgcggcgc cgccgggctg cagtgacgcc
     7321 gagaggccgg cgcgcgggct tcggcagcgc cgcgctcctc gccgccgctt cgggatcgat
     7381 gcgttgcggt cgcctcgtca atcgcttccc gcgcccttct cggccctctc gcccttcgcc
     7441 gccaggtaga tgcccaccag cagcaggctg aagcccacga actgcagcgg cgcgaagcct
     7501 tctccgaaga cgaggaaggc catcagcgcg ctgcccaccg gctcgcccag cgtgaccacc
     7561 gcgacgaagg tggcagacac gtagcgcagc gaccagttgt aggcggtgtg gccgagcagt
     7621 tgtggtccga gcgccatcgc cagcagcacc agccaggcgg tgttcgacag gctgaacagt
     7681 tccacgccgc ccgcgccgct ggcggcgaac aggaacagcg ccgcggcgcc gtaggcgagc
     7741 cagatgtagg ccggcagccc gaggccggcg cgcaggcggc ggccgatcag caggtaggcg
     7801 gagaagcacc agctgccgac gagcgcgagg aggttgccga acatggggtt gctgccggcg
     7861 ccggcggtct ggctgtcgct ccagaagatg aacaggctgc cggcgaagga cagcgcgatg
     7921 ccgccgatca tcatcttcgt cggcgtctcg cggaagatga gaaaggaggc caggccgatc
     7981 cacagcgggt tggtggtgac gagcgcggtg ctcgaggcga ccgaggtgta ttcgagcgag
     8041 ctgatccagg tggcgaagtg gagcgcgagg aagaagcccg cggccagcgc catgccgatc
     8101 tgcctgcggg tgaggcgggc gagctcatgg cgcgattgca ggaaggcgac cggcgtgatg
     8161 atgagcgcgg cgaagcccag ccgccaggtc gcgaccgcga gcgagctcac cccctcggcc
     8221 tgggcgaggc gggcgaagat ggcgccgaag gagatcgcgg cgaggccgat gccgaggacg
     8281 acgaagggga gccagcgggc gggttggggg gtgtgggaca tctggagagg ggcgattggt
     8341 gggtgaggtg ggtcgagagc accctgaggg gcttcgaccg gtggcgatgc gtgttcgaac
     8401 cgttcgcggg gacggcacat gtgtgccggc actcgggtgc ttccggaggc gtggcggttt
     8461 tccgtaggag cgacgcaagt cgcgatcggg cagtgccgca gcgccgtcgc gatttccgcc
     8521 cgctgcaatc gcgacctgcg tcgctcctac ccgacgcatt gcggcgcgat tgtgccgcat
     8581 cgcgtcgaaa ccgcggcgtt tcgtctgatc gcggagcggc cccggttggc gccattcctc
     8641 tcgcgtcgca cgcctcacgc gcctctcagg cgtggccgcc ctccagatac gccgcccgca
     8701 cctccggact cgccagcagt tccgccccgg tcccggcgag ggtgatctcg ccgtgctgca
     8761 gcacatagcc gcggtcggcc acgcgcaggg cctggttggc gttctgctcg accagcagga
     8821 tggtcatgcc gtggtcgcgg ttgagctccc tgacgatctg gaagatcttg cggatgtaga
     8881 tcggcgccag gccgagcgag ggctcgtcga gcagcagcag cttggggcgg ctcatcagcg
     8941 cgcggccgat cgccagcatc tgctgctcgc cgcccgacag ggtgccggcg cgctgcgcct
     9001 ggcgttcctg caggatcggg aacatcgcga acacgcgcgc ggtgtcggcg gcgtagttgg
     9061 ccgggtcggc gtgcgcggtg cccatctgca ggttctcgat caccgtcatg cgcgggaaga
     9121 tgcgccggcc ttccggcacc agggcaatgc cgcggcgggc gatctcgtgc gtgtgcacgc
     9181 gggtgatgtc ctcgccgtcg aagacgatgc gcccggcgct ggccttgggg ttgccgaaca
     9241 ggctcatcat cagcgtcgat ttgccggcgc cgttggcgcc gatcagggtg acgatctcgc
     9301 ccacctggac ctcgacgtcg atgccgcgca gcgcgtggat gtggccgtaa tgggcgtgca
     9361 cgccctccat cttgagcatc atgccgcggc ccctccagtc acttcttctt cctcgtcctc
     9421 gcccaggtag gccttgatca cctgcgggtc gtgcttgacc tgcgccggcg tgccctcggc
     9481 gatcttgcgc ccgtagctga tcacgccgat gtggtcggac acgttcatca ccacgctcat
     9541 gtcgtgctcg atgagcagga tcgcgatgcc catctcgccg cacaggtaga gcaggagttc
     9601 gttcagctcc gccgactcgc gcgggttgag gccggcggcc ggctcgtcca ggcacagcag
     9661 caccggatcg acgcacagcg cgcgtgcgat ctcgatgcgg cgctggatgc cgtagggcag
     9721 gtcgccggcg gcgtcgtcgg cgaggtgggt gagcttgagg cgctcgagcc acatcgccgc
     9781 gcgttcgatc gcctgccgct ccgcctggcg gtagccgggg agcttgaaga taccggcgat
     9841 ggagaacttc gaggcgcgct gcagcacgtt gtgctgggcg acgatcaggt tctccagcac
     9901 cgtcatcttg gggaacaggc ggatgttctg gaaggtgcgc accacctggg cgtcgcgtgc
     9961 gacgcggtgg ctgggcaggg actccaggcg catctcgccg cgcgtggggt gggccaggcg
    10021 cagcgtgccg gtggtgggct tgtagaagcc ggtgaggcag ttgaacagcg tcgtcttgcc
    10081 ggcgccgttg gggccgatga tgccggtgat cttgccggcc ggcacttcca gcgagaggtc
    10141 gtcgatggcg gtcaggccgc cgaagcgcat ggtcaggttg cgcaccgaca gcagggttgc
    10201 gggcgtgttc atcgcgcagc ctccttcgtc gcggcagtct tgcccgacgc gttcaggtgc
    10261 accgtcggtt cgcggtgcga cagcaggccg cccgggcgcc acaccatgat cagcaccatc
    10321 gccaggccga acagcaacat gcggtactcg gcgaagttgc gtccgaactc ggggatcagc
    10381 accagcagcg tggccgccag caccacgccc atctgcgagc ccatgccacc gagcacgacg
    10441 atggcgagga tgatcgccga ctcggtgaag gtgaagctct cgggcgagat gaagccctgg
    10501 cgcgcggcga agaacacccc ggcgaagccg cccagcatgg caccgatggc gaaggccgag
    10561 agcttgatgt tggtggtgtt catgcccatc gcctggcagg cgatctcgtc ctcgcgcagc
    10621 gcctcccagg cgcgccccac cggcagcttg cgcaggcgcg acacgaaggc gtgggtcagc
    10681 agggccagca ccaggatcag gtagtagagg aagatcaggc ggtgcatcgg cgagaattcg
    10741 aggccgaaga agccggcgaa ggtctgcccg ccttccggcg cgctgcgcgt gaaatcgagg
    10801 ccgaagaagc tcgggcgcgg gatcgagctg atgccgttgg ggccgttgga gaattcggtc
    10861 caattcacca ggatgatgcg gatgatctcg ccgaagccca gcgtgacgat cgccaggtag
    10921 tcgccgcgca ggcgcaggat cgggtagccg agcaggatcc cgaagctggc ggcgagcgcg
    10981 cccgataccg gcagcgcctc ccagaagctc cagccgaact gggtcgacag cagcgcgtag
    11041 gaataggcgc ccaccgcgta gaaggcgacg tagccgaggt cgagcaggcc ggcgaggccg
    11101 acgaccacgt tgaggcccca acccagcatc acgtagatca gcaccgtggt ggcggtgtcg
    11161 accacgtagc ggttgtcgga gaacacgatc ggcagcgcga tcgaggcgcc gagcgccacc
    11221 cagccgagca cgttcaccac gcgctgcgcg ccatgggcgc ggtcggcggc ggtggcggtg
    11281 ctggcgcggt cgcgttcctg cttgcggttg cgcgcctggt cgatcgccca gcgcagcagc
    11341 aggcggccgg cgaacaccgc ggccacggct gcggcgagca ggccgaagcg ggtctccacg
    11401 cccagccggc cgcccacgtc caccgtggtg aggccgacca gcgggatgcc caggatgagg
    11461 ccgacgaagg ccacccagcc ggcgtcgcgc agacgctcgg ccggcgtcat cgcgtgacgc
    11521 gcaaagacga cggcgctcat cagaccttct ccacttcagg ccggccgagc aggccggagg
    11581 ggcgcagcat cagcaccagg cacaggatgc cgaaggtggc gacgtccttg tattccgccc
    11641 acaggtaggc ggcccagaag gattcgatca agccgatcag caggcccccg agcattgccc
    11701 ccggcagcga gccgatgccg cccagcaccg cggcggtgaa ggccttgatg ccggcgagga
    11761 agccgatgaa gaagtccacc acgccgtaat acaccgtgac catcaccccg gcgaccgcgg
    11821 cgagcgccgc gccgagcatg aaggtgtagg agatggtgcg gtcgacgttg accccgagca
    11881 gcgcggccat ggtgcggtcc tgctcgcagg cgcgctgctg gcgaccgaag ggggtctgcg
    11941 tgatcatcca ggtgaaggcg ctcatcagga tcaccgtggt gacgatgatc agcatctggc
    12001 tccagccgat ctgcaccgtg aagcccggcg cctcccacag cacgatgccg ccttcgagca
    12061 ccggcgggat cggcttcacg cgtgcgccct gggtgagctg cacgctgttc tgcaggaaga
    12121 tcgacatgcc gatcgcggaa atgagcggcg ccagccgggt cgagccgcgc agcgggcggt
    12181 aggcggtgcg ctcgaccgcc cagccgtagg ccgaggtgaa gaagatcgac acgatcagca
    12241 cgaaggtgag cgcgagcggg atcgaggtga cgtcggccgc ggcgaggatg gtgaaggcgg
    12301 tgaccgcgat gaaggcgctc accatgaaga tgtcgccatg ggcgaagttg atcatgccga
    12361 tgatgccgta caccatcgtg tagccgatgg cgatcaggcc gtacatggcg ccgagcgtga
    12421 gcccgttgat cagttgctgg agtgcatagg ccacgatgtc tcctgctgtg gcggtgcgga
    12481 tggcgcggat cccgctcggg gccgcgtccc tgtaggcgcg gcgcaagccg cgatcaagcg
    12541 ctggatcgga agtcgtcgct cgcaacccgt cgccggcccc ctgtctcctt gttggccgac
    12601 gggggcacgg acaccggcgc cgcgattgcg gcgccgaaga ctcgggtggg acgggcttgc
    12661 gacttacttc gcgtagtcgt acttgccgcc ctgccagcga tagaccacga agcccggcgc
    12721 cttctggtcg cccttggcgt cgaaggcgag atctccgatc acggtcttga agctgccgcc
    12781 gcgcatcgcc ttctcgaggt cggcgtactt ggtgctcttg gccgtggtgg cggcctgctc
    12841 gaagagctgc atcgcggcgt aggcgtagag cacgtagcct tcgggctcgg tcttggcggc
    12901 gcggaacttc tccaccaccg gcgcggcgtc cgggttcttg cgcgcgtcgg gcgagaacgt
    12961 gaacatcacg ttgtcggcgg ccgggccggc ggcggtgacg agctcggagt tggtcatcgt
    13021 gtcgccgccc atcacggtga gctgcaggcc ggcggcctgg gcctggcgca ggatcagcgc
    13081 gacctcggtg tggtagccgc cgtaggccat gacctcgacg ccggcctgct tgagcttggt
    13141 gacgatgcca gagtagtcct tctcgccggc ggtgatcgat tcgcgcagcg cgggcttggc
    13201 gcccttggcc tccatcgcct tggccatctc gtcggcgagg cccttgccgt aggcgctctt
    13261 gtcgtcgacc acggcgacct tcttgccctt gaagtggtcg gcgatgtagc cgccggccac
    13321 caggccctgc tggtcgtcgc gacccatgat gcggaagatg ttcttcaggc cgcgctcggt
    13381 cacctgcgga ttggtcgaca ccgtggccat cgggatgtcg gcctcgacat agacctcgga
    13441 ggccgggatc gtcgagctcg agcaccagtg gccgtgcatg aacacgatgc ccttgttgac
    13501 catggtgttg gcgaccgaca ccgcctgctt cgggtcgcag gcgtcgtcgc cgacctcgag
    13561 cttcagtttc tggccgagca cgccgccgcg ggcgttgatg tcggcgatgg ccatctccgc
    13621 gcccttgcgg atctggtcgc cggcggaggc gtactggccg gtcatcggac cggcgatggc
    13681 gaccgcgagg tcggccaggg cggcattcga aaagccgccg agtgcgagga cggcggtggc
    13741 gagcagggtc tttttcatga aacgtctcct tcgtggctgt ggttttggca cttgaacggg
    13801 gcagacggcg ccagatgggg ccgcctgcgt ccggcaaagg taagcgaatt tttcagttct
    13861 gccatccgtg atggccctag ctgcgactac cgcgcaggtt cgttgtcatc cagcacccct
    13921 ccgggggcat ccggaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
    14041 actaccgcgc aggttcgttg tcatccagca cccctccggg ggcatccgga agcgcgttgg
    14101 ttttccgtag gagcgacgca agtcgcgaat gcaaccacgc aagtcgcgaa tgcagcggct
    14161 ccgatggcgc cgcgccggat tccgtgcgct gaagatcgcg acttgcgtcg ctcctgcggg
    14221 caggtccact cctcgccggg ttgccagcgc tgccgcggtg cgggcgcgaa tcgcgcacac
    14281 tcatgccccc atcgccatca ggctggcgtt gccacccgcg gcggcggtgt tgatcgacag
    14341 ggtgcgttcg tgcaccaggc ggccgaggtc gtagtcgggg cagggctgca gcagctgcag
    14401 gatcgggccg ctgcgcccgg cgacgcgcac ctgcagcgcg tcggcctcgg cgtcgctgcc
    14461 gtcgaacagc accgcgccga gcgccttgtc gaaccaggcg gcatccaccg cgacgtatgc
    14521 gcgcagcgcc tcggggagcg cggcctgcac cgtgcgtgcg gcggcgcggt cggcgagcag
    14581 caggcgattg ccgctggcga gcgccgccat cacctgatgc agcacggcgg ccgcgctgtc
    14641 cgccacgccg gcgatggtgc cgcgcgcggt gaagcgcagg ctgtcgtcct cgccggtggg
    14701 gccgggcagc gccaggcgca ggccagcaat acggcgcgca cggcaggcgt cggcgcggtc
    14761 gcgtagcgcg gcgagctcgt cgccctcgag caggccgcgg ccggcgccgt ccagccattc
    14821 caggaagccg gagaacgcgg catccgcggc gcgggtttcg tcgctgtcgg tcaccgcgag
    14881 cgcgccatcg gccagcaccg ggccgggcgt acgcgcaagc agccggtgca ggtagagcgg
    14941 tccgcccgcc ttcgggccgg tgccggacag gccctcgccg ccgaagggct gcacgcccac
    15001 caccgcgccg atgatgttgc ggttcacata caggttgccg acgtgtgcgc gcgcggcgac
    15061 gcgctccacg gtctcgtcga tgcgggtgtg cacccccatg gtgaggccgt agccgctggc
    15121 gtcgatcgcg tcgatcaggc tgtccaattc ggacgcgcgg tagcgcagca cgtgcaggat
    15181 ggggccgaac tgctcgcggc ccagttcgct cagggcaccg atctcgatga tcgacggcgc
    15241 gacgaaggtg ccgtgcgcgc atgcggcggg cagcggcagg cgggtgatgc gggcgccctt
    15301 ggcaagcatc gcggcgacgt gggcctcgag accgtcgcgc gccccggcgt cgatgaccgg
    15361 gccgatgtcc acgcgcacgt ccgccggatt gcccaggcgc agttcggcga gcgcgccctg
    15421 cagcatgtgg aggacgtcgt cggcgatgtc ctgctgcagg cacagcacgc gcagcgccga
    15481 gcagcgctgg ccggcggagt cgaaggcgga ggcgaggacg tccgtcacca cctgctcggg
    15541 gagtgcggtg gagtccacga tcatcgcgtt ctggccgccg gtctcggcga tcagcggcac
    15601 cttgccgcca cgcgcggcga ggctgcggtt gatcagcgcg gcgacctcgg tggagccggt
    15661 gaacatcacc ccacgcacgc gcgcatcggc caccagcgcg gcgccgacgg tctcgccgcg
    15721 gccgggcagc aactgcagcg ctgcagccgg gatgccggcc tcgtgcagca ggcgcactgc
    15781 atgggcggcg atcagcgggg tctgctcggc cggcttggcg agcaccggat tgccggcggc
    15841 gagcgcggcg gcgacctggc cgctgaaaat cgccagcggg aagttccacg ggctgatgca
    15901 cagcaccgga ccgagcgcca cgtggctggc agggtcgaag ccacgcgcct gctgggcgta
    15961 gtagcgcagg aagtcgaccg cctcgcgcag ctcggccacc gcgttggccc acgacttgcc
    16021 ggcttcgcgc actgcaagcg cgagcagggt gtcggcctcg cgttcgaaca gcgccgcggc
    16081 gcgctccagg cgttcgctgc gcgccgcagc cggggtggcg gcccagtcgg ctgcggccgc
    16141 actggcggcg gcgagcgcgt gctcgaggtc gtcggtcgtc gcttcggcga cgccgccgac
    16201 gagctcgccg cggtcggccg ggttggagac cggggtcacg tccgcgggca ccgcgctgcg
    16261 gccggcgacg atcggaccgg ccgcgaaggg cgtggtgcgc gagttggcca gcgccgcggc
    16321 gatgcgggcg cgcaccggct cgctggcgag gtcgatgccg gcggagttgg tgcgcaggcc
    16381 accgaacagc tcggcgggca gcgcaatctt cggatgcggc gcgccggcga gcggggcggc
    16441 ctgctcgacc gggtcggcaa tcagctcctc caccggcacg cgctcgtcga cgatgcggtt
    16501 cacgaagctg gtgttggcgc cgttctcgag caggcggcgc accagatagg cgagcagggt
    16561 ctcgtggctg cccaccgggg cgtagatgcg caccaggcgg tgacggtcgg cgcggccgac
    16621 gatctggtcg tagagcggct cgcccatgcc gtgcagacac tggaactcgt agtcgcccgg
    16681 ctgccatgcg cggccgtcgg cggcggcgag gtggaagatc gccgccagcg aatgtgcgtt
    16741 gtgggtggcg aactgcgggt agatgacgtc gcgtgcggcg agcagcttct tcgcgcaggc
    16801 gaggtaggac acatcggtgt acaccttgcg cgagtacacc gggtagccgg ccagcccgtc
    16861 gatctgcgcg cgcttgatct cggcgtccca gtacgcgccc ttgaccaggc gcaccatcat
    16921 gcgctggccg ctgcggcgcg cgaggtcgat gacgaagtcg agcacgtggg gggcgcgctt
    16981 ctggtaggcc tgaaccacga agcccagtcc cgtccagccg gcgagcgcgt cgtcggtggc
    17041 gagcgcctcg agcaggtcga gcgagaggtc gaggcggtcg gcctcctcgg cgtcgatgtt
    17101 cagcccgatg tcgtggcgcc tggcctgcag gcacagggtc ttcaacttcg gcagcagctc
    17161 ggccagcacg cgctcgcgct gcgaccaggt gtagcgcgga tgcagggccg agagcttgac
    17221 cgagatgccg ttgccgtcga ccacgccgcg gccccgggag gcgacgccga tggcctggat
    17281 ggcgcgctcg tagtcgcgga aatagcgctc tgcgtcgctt gccgtcatcg ccgcctcgcc
    17341 gagcatgtcg aaggagtagc ggtaaccctt gttctcgtgc tcgcggccgc gctcgagggc
    17401 ctcgtcgatg tcgcgcccgg tgacgaactg ctcgccgagc atgcgcatcg ccaggtccat
    17461 gcccttgcgg atcagcggct cgccgccgcg cgccaccagg cgggtgagcg cggaggacag
    17521 gccttgttcg ctgctggtcg acaccagccg gccggtgatc agcaggcccc aggtggcagc
    17581 gttgacgaac agcgaggggc tgttgccgat gtgtgcgcgc cagtcgccgt cggcgagctt
    17641 gtcgcggatc aggcggtcgg cggtggcctt gtcgggcacg cgcagcaggg cctcggccag
    17701 gcacatcagc gccacgcctt cctggctgga gagcgagaac tccttcatca gggcgtcgac
    17761 gccggacgag cgcgtgcgcg tggcgcgcag gccgacgacg aggtcgtggg cgagggcgcg
    17821 cacgggttcg cgcagggccg cggggaggcg ggcggcctcg acgagcgcgg gcacgcactc
    17881 cggctcgggc gtgcgccaag cggcgtcgat ggcgtggcgg acggtgctgc gcgcggtcgg
    17941 cggaagggcg gcggcggcat ctagggccgc tgcggcgcgg gcgctggagg ggatgttgtc
    18001 ggtcacggcc gggcactcct ggcattgcgc acgctgcgtc gggcgggcgt gctgcaagcg
    18061 gttgcggggg cggcggaaga cccgccggaa tggccgaatt cttcgccaaa aaccgtagtt
    18121 aatggctcca aaatttgcat ttatatggtc ataatgacca aagtgagatc acttcacagg
    18181 ccaatcgcct gctgcaccgc aatggaccgc atcgaccaga agatcctcca ggaattgcag
    18241 gccgacgccc gcctgtcgat cgtcgagctg tcgcgccggg tcggcctgac caagaccccc
    18301 tgcgccgagc gtatccgccg cctcgagaag tccggggtga tccgcggcta ccacgccgcg
    18361 ctcgaccccg acgcggtcgg cgccggccac atcgtcgtcg tccaggtcct gctcaccagc
    18421 actaccgagc aggacctgcg ccgcttcaac gaggccgtgc ggcgcatccc cgagatcgag
    18481 tcctgcctga tgatcgcggg cgacttcgat tacctgctca aggtgcgcac gcgcgacatc
    18541 aacgaatacc gccgcgtgat gggcgagcag atctccggcc tgccgtgcgt caagcagacc
    18601 catacctacg tggtgatgga ggtggtcaag gacgagcgca agctgccgct gcgcggcagc
    18661 gcgtgaggcg cccggggggc gacgcggact cttgagcacg gtcaacacgg gcgcgaagtt
    18721 gcgtcgcaca atcgcgcatg gtcccggacg atcgacgctg cgggcatgtc gtgcgcggat
    18781 ggtggcgcgg agcttgcctc gatggcgcaa caaaggtgca agtgatggac tcgaagcgtg
    18841 caatgccctc cctcaccatc ggtgccatcg gcgtcgtgtt cggcgacatc ggcaccagcc
    18901 ccctgtacgc cctgcgggag atcttcaacg gccaccatcc gataccggtc acgccgggga
    18961 acatcctcgg catcctgtcg ctcgtgctgt gggcgatcat ggtgctggtc acgctcaagt
    19021 acgtgctcat cctgatgcgc gcggacaacc gcggcgaggg cggctcgctc gccctgctgt
    19081 cgctggtggt gacgcatgcg cgcagcgcac gcctggcctg ggtggtgtcg atgctcggca
    19141 tcttcgccgc cgccttgttc ttcggcgaca gcatgatcac gccggcgatc tcggtgctgt
    19201 cggcggtgga gggaatcggc atcgtggcgc cggggctcga ggcctgggtg gtgccggtga
    19261 cggtgctcat cctcaccgtg ctgttcgcga tccagcgccg cggcaccggg gtggtggggc
    19321 tcttcttcgg cccggtcatg gtggtctggt ttgccgtgct gggtgcgctc ggcatggtcg
    19381 agatcgtgcg ttatccggag atcctcgcgg ccctcagtcc gctccatgcg gtcgccttcg
    19441 tcgtcgatca tccggcgctc gccttcctcg cgctgggcgc ggtcgtgctc gcggtgaccg
    19501 gcggggaggc gctctacacc gacatgggac atttcggccc ggccccgatc cggctggggt
    19561 ggttcggctt cgtgttgccg gccctggtgg tgaactactt cggccagggg gcgctgctgc
    19621 tgcacgagcc ggcggcgatc gaaagcccgt tctaccacct cgggccgtcg tgggcgatga
    19681 tcccgctgct ggtgctcgcc accctggcca cggtcatcgc ctcgcaggcg gtgatctcgg
    19741 gcgccttctc ggtggcccgc caggcggtgc agatggggct gcttccgcgc atgctgatcg
    19801 tccatacctc ggggcatgag caggggcaga tctacgtccc cttcaccaac tggacgcttt
    19861 acctggcggt ggtcgcactc gtggtcggct tccagaactc gtcgaacctc gctgcggcat
    19921 acggcatcgc ggtgaccggc acgatgctga tcgacacggt gctgctcggc ttcgtgatgg
    19981 tgctgatctg gcgctggcgt ccgctgctgg ccggactgtg cgcggggacg ctgctgctgg
    20041 tggacctcgc cttcttcagc gccaacgcga tcaagattcc ccagggcggc tggttcccga
    20101 tcgccatggc gctggtctcg ttcaccgtgc tcaccacctg gcgccagggg cgtcgcctgg
    20161 tgcagcagga gatcgccgcc cagggcctgc cgctgaacgc gttcatcgac atggtcgggg
    20221 aggtgcatcg ggtgcagggc acggcggtgt tcatgaccag caccaaggat ggcgtccccg
    20281 ccgcgctgct gcacaacctc aagcacaacc aggtcctgca cgagcgggtc gcgctggtga
    20341 ccgtggagac cgccgacacc ccgcatgtga gcgagctcga ccgcatctac ctgcatcgca
    20401 tcaggaacgg gttcatgcgc gtgatcgtgc gctacggctt catggaggat cccgacatcc
    20461 cgagtgcgct gaagcactgc agccgcttcg gcgaatcgtt cgagatgatg gagaccacct
    20521 tcttcgtctc gcgcgagacg gtgatcccca ggctcagccg caggctgttt tccgactggc
    20581 gggcgcggct gttcgccttc atgtccaaga acgccaccag cgcgaccgac ttcttccgca
    20641 tccccaacaa ccgggtggtc gaactcggca cgcagttggt gatctgacgc ggccacgcgc
    20701 catcggggcg gccggaaggc gcccccgggc tttggtgtaa cattccggcc aaagcatcgg
    20761 ctgccgcccg ccgggtggcg gccccctcac gagagacacc caatccatgg ttccgcacct
    20821 caccaccgcg cttaccggcc ccctgctgga gctggagcgt cagttcctcg acaacgcctg
    20881 cgacatcgaa aagtggttcc gtacccagtg gcaggaccac atgccgccct tctacggttc
    20941 caccgacctg cgcaactcgg gcttcaagct cgcgccggtc gacctgaacc tgttcccggg
    21001 cggcttcaac aacctcaacg acgtcttcat gccgctgtgc gtgcaggcgg cgcaggcggc
    21061 gatcgagcgc atctgcccgg acgccagcaa gctgctgttg atccccgaga accacacccg
    21121 caaccagttc tacctgcaga acgtcgccaa gctggcgtcc atcctgcgcc tcaccggcct
    21181 cgacgtgcgc atcggcagcc tgctgcccga gatcgccgcg ccgaccgtgc tggaactcgc
    21241 caacggcgcc acgctgaccc tggagccgct gcgccgcagc ggcagccgca tcgggctcga
    21301 cgacttcgat ccctgcgcgg tgctgctcaa caacgacctg tctgcgggca ttccgcaggt
    21361 gctcaagggc ctcgacgagc agtggctgat cccgcccctg catgccggct ggcacaagcg
    21421 ccgcaagtcg aagcacgccg agtgctacga ccgtgtcgcg cgtgagttcg ccgccgcgat
    21481 cggcatcgat ccgtggcgga tcaaccccga gttcggcgtg tgcggccaga tcaacttcca
    21541 ggaacgcacc ggcgaggagt gcctcgccgc tcaggtggat tcgctgctca cccgcatccg
    21601 cgccaagtac aaggaatacg gcgtcaccga cgagcccttc gtggtggtca aggccgacgc
    21661 cggcacctac ggcatgggcg tgatgacggt gaaggacgcc tccgaggtcg tcggcctcaa
    21721 ccgccgccag cgcaacaaga tggcggtggt gaaggaaggc ctgcaggtgc acgaggtcat
    21781 catccaggag ggcgtgcaca ccttcgagac ggtggaggac ggcgtcgccg agccggtcgt
    21841 ctacatgatg gatcactacg tggtgggtgg cttctaccgc gtccacaccg agcgcggccg
    21901 cgacgagaac ctcaacgcgc cgggcatgca cttcaagccg ctcgccttcg acaccagcgg
    21961 caccatgccc gatgccgcgc aggggcccga tgcgccgccg aaccgcttct acgcctatgg
    22021 cgtggtggcc cgcctggcgc tgctcgcggc ctcggtggag atcgaggaga ccgccccggc
    22081 cgaggaagag gatgcaggtg gagacgccct ggcggcctga agtctcgcgc cgcctccgac
    22141 gggaatcgac acgaaccggt gatgaatcca tgacccgcaa gctcaagctt cttttcatcc
    22201 tcgacccgct cgagggcctc aaggcctaca aggactcgag catcgcgatg atgcgcgccg
    22261 cggcgcggcg cgggcacgag gtgtgggcgc tgcagcgcgc cggcctcacc tggcgcgagg
    22321 gcgtggtggt tggcaccggc tgcgtggcgc tcgcggtgtg cgacgacgac gcggcctggt
    22381 atcgcgcggg cgaggcgcag gccctgccgc tggccgcctt cgacgcggtg ctgatgcgcc
    22441 aggacccgcc cttcgacttc gagtacgtca ccgccacctg gctgctcgag cgcgcggcgc
    22501 aggccggcgt gcgcgtgttc aacgacccgc gtgcggtgcg cgaccactcc gagaagatcg
    22561 cgatcaccga attcccgcag ttcaccgcga ccacgctggt ggcgcgcgac ccggcggaca
    22621 tccacgcctt catcgacgag ctcggcgacg tcatcctcaa gccgcttgac ggcatgggcg
    22681 gcagccagat cttccgtgtg cgcgccgacg accccaaccg caacgtgatc gtggagacgc
    22741 tcgcccacga tggcactcgc accatcatgg cgcagcgcta cctgccgcag atcgtcgacg
    22801 gcgacaagcg cgtgctgatc atcggcggcg aggtcgtgcc cttcgcgctc gcgcgcatcc
    22861 cgcgggcggg cgagacgcgc ggcaacctcg ccgccggcgg gcgcggggtg gcgatggcgc
    22921 tcaccgcgcg cgaacgcgag atcgccgggt tcctcgcgcc gacgctgtgg gcgcgcggcc
    22981 tgctgatcgt cggcctcgac gtgatcggcg gccacctcac cgagatcaac gtcaccagcc
    23041 ccacctgcat ggtcgagatc gccgcgcagc agggcttcga cgtcgccggc ctggtgatcg
    23101 acaagctgga gcgtgcgtgc ggctgaacct gcgcatcctt gcattggccg cgtgcgcgct
    23161 gctgctcgcg gcctgctcgc gcgcgcaggt cttccatcac gaggcctacg tcttcggtac
    23221 ccgggtcgat gtcgcggtgt atggcgagaa ccaggccgag gccgaccgcg ccgccgccgc
    23281 ggtgctgcgc gagttcgacc gcctgcatcg cgcgtaccac gcctgggagc cctccgagct
    23341 caccgcgctc aacgccgcga tcgcgcgcgg cgaggccgcc accgtgtccg acgagctcgc
    23401 cgcgatgctg aaggacgcgc aggcgatcgc cgccaccggc gacgggctgt tcgaccctgc
    23461 gctcggcggc ctggtcgcgc tgtggggctt ccacaccgac accttcgtgc cgcagcgccc
    23521 cgaccctgcc aagctgcagg ccctgctcgc cgcgcacccg tcgatggccg acctcgtcct
    23581 cgacggcaat cgggtcgaga gccgcaaccc ggcggtgcag ctcgatctcg gcggctacgc
    23641 caagggctac gcgctcgacc gcgcggcggc gatcctgcgc gcgcagggcg tggccgacgc
    23701 gctcatcaac atcggcggca acgtcatggc gctcggcgcc aagggcgacc agccctggcg
    23761 gatcggcatc cagcatccgc gcgcgcccgc tccgctcgcc accctgcctt tgtacgacgg
    23821 cgaggcgatc ggcacctcgg gcgactacca gcgctacttc gagctcgacg gcgagcgcta
    23881 cgcccacctg ctcgacccgc gcaccggcca gcccgcgcat ggcacgcagt cgctcaccgt
    23941 cctgatcacg ccgcgcgcca atgccggcac gctgtcggat gcaccgagca agcccgccta
    24001 cctcgccgga gatggctggc gcgaacagat ccgccgcttc ggcatcgaac atgcactgcg
    24061 cgtggctgcg gacggccgcg tcgaggtcac ccgcgcgttg cgcgcgcgcc tgcagttccc
    24121 gtcgccggtg gaggcgacgg tggtcgattg agatccacaa aggagggagg acaacatgga
    24181 catgaccccg caaaccgcct ggctgctcgc catttccgcc gtcgtcatcg tcggcgtgat
    24241 cgcgttcttc gtcgggcgtt cgagcgccgg taccaagggg cgcatcgacg agctcgaggc
    24301 cgagctggcg cgcaaggccg aggaggcaga gcgctaccgc aaggaggtcg acgcccattt
    24361 cgacaagacc gcgaccctgt tcgtgtcgat ggccggctcc tacaaggagc tcttcgagca
    24421 cctgtccgcc ggctacgaca agctctccgg cgcctcggcg cgcgagcgct tccaggagcg
    24481 cgtcgatgcg ctgctgcccc gccgcgtggt cgagcccgcc gcggccggcg cgctgctcgc
    24541 ggggggagcg gcgggcgctg ttgcgggtgc ggacgccgcg gaaccgacgg gatcgagcgc
    24601 ggatgcagtg gtcgggcccg cgccggtcag cgtggtggat gcggcccctg ttccggaggc
    24661 ggaggatctc gccgaatccg tcccgccaag cgtcggggcg acttccggag tgtctgcgga
    24721 cgaggcggag aaggctgcag ccgagtcggt cgccgcacct gccggagccg aggaacccgg
    24781 aagtgctggc gccgatgcgg atgccgcgga gcgcgtccgt cgtgccgagg gggcggccgc
    24841 cgagaccgtc ggcgagtcgg gcgccgagtc ccgcggcgag acgcgctgag cggggcgtgc
    24901 gcgggcggac ggagcgggcg aacttgcccg ctgccggtgc ggtcacaagc cccatgtccg
    24961 cccgtgcgct ccaccgtctc ttcctgatcg cctgcagcgc gttgctgctc gtcggttgcg
    25021 gcctgcgttt cgcgtactcg cagctcgact ggctgctgcc ttggtatctg cgcgactacg
    25081 tcacgctcga cgccggccag cgtggcgaat tcgaccgccg tctggccggc ctgctcgact
    25141 ggcactgccg cagccacctc cccgagtacg tcgccctact gcgtgcggcg aacgcgacgc
    25201 tggcggccga gcgcgtcgag cctgcgcagc tcgaacgctt cctcgagcgc ggagaggcgc
    25261 tctggcgcga gatcgtcggg gaactcgagc ccgagctgcg caggctggcg gccggcctgg
    25321 gcgacgagca ggtggaggag ctcgccgccg ccttcgtccg ccgtggcgag gaggcgcgcg
    25381 cggagttcct ctccggcgac gagtccgcgc agcatgccgc ccgcgtcgag cgcatggagg
    25441 agcgcctgcg gcgctggttc ggccgcatga cgccggccca gcgcgagcgc atcgcggcgt
    25501 ggagccgggc gctgcagccc acgaccgagg cctggctgga ggatcgcgcg cgctggcagg
    25561 ccgagctgct cgacgccttg cgcgtgcgcg cggacgccgc cgccttcgcc ccccgcctgg
    25621 cgcaggcgct ggcgccgcgc gaggcgcgct ggagcgcgca gcatcgcgcg gcggtcgccc
    25681 acaaccgcgc acgcacgctg gagctgctcg ccgaactgca tgcattgtcc agcgcagcac
    25741 aacgccgcca gctgcgtgac gagatcgaca tgctcgccac ccagttcgcg ggcataagct
    25801 gcgccgaacc ggctcgcgtt tccgcggctg gcggccgata atcgcgccct tcgtctaccg
    25861 ccgcccgcgc cctggtgcgg gctctcccgc atgcacaaga ccgaactcga ccgtctcgcc
    25921 atcggcctca tggtggtgct gtgctcgatc tggggcctcc agcaggttgc catcaagctt
    25981 gccagcgccg gcatctcgcc ggtgtggcag gccggcctgc gctcgatcgg cgccacggcg
    26041 atcgtcgtcg tttgggcgct gctgcgtggg gtgaagctct tcgagcgcga ccgcagcctc
    26101 gcgcccggcc tgctcgccgg ggcgctgttc gccggcgagt tcgcgatgat cttcctcggc
    26161 ctgcagcaca ccagcgcctc gcgcggggtg atcttcctgt acaccgcgcc cttcttcgtc
    26221 gccctcggcg cggtctggct gctgccgcag gagcacatgc ggaggcggca gtggatgggc
    26281 atggcgctcg ccttcgtcgg cgtgctcgcg ctcttcggcg agaacctctt cgccgccacc
    26341 gagggcgcct gggtgggcga cctcatgctg ctcggcgccg cgctgctgtg ggcggcgacc
    26401 acgctgaccg tgaaggcgag cgcgctggcg cgcgcatcgg ccgagaagac gctgctctac
    26461 cagctcggcg tgtcggcgct ggtgctgccg ccgctctcgc tggcgatggg cgagccgggc
    26521 gtgttcgcgc ccacgcccat ggtgtgggcc aacctcttct tccaggcggc gatcgtcgcc
    26581 ggcgtgtcgt atctggcctg gttctggctg gtgcgcgagt atccggccac gcgcctgtcc
    26641 tcgttctcct tcctgacccc ggtgatgggc gtgctcgccg gcgggctgat cctcggcgag
    26701 ccgctcacgc cggcggtgta cgccgcgctc ttcctcgtcg gcagcgggat ctgggtggcg
    26761 aaccggtcgt agcgtgaccg gctgcccggc tgcactccac tctcttgcgc gtgcgggctt
    26821 ggcgccccgc ggtggggcgg ggcgcggggc gatcgcctat agtgacggcg tccaccgtct
    26881 tccgaggagc ctccccatgt cgcgacccgt cgtcgagttc tggttcgagt tcgccagcac
    26941 ctattcctac ctgtcggtga tgcgcatcga gcgcgcagcg gccgcgaccg gcgtggacat
    27001 cgaatggaag cctttcctgc tcgggccggt gttcctcgcg ctgggctgga acgactcgcc
    27061 cttcaacatc tacccgccca aggggcgcta catgtggcgc gacctggaac gtctggcgga
    27121 gaaggagggc ctgcccttcc gccaccccag ccgcttcccg cgcaacggcc tgctcgccgc
    27181 gcgggtggcg ctggtggggg tggaggaggg ctgggtggcg cccttcgcgc gtgcggtgat
    27241 gagcgcgaat ttcgccgagg accgcgagat cggcgaggag gcggtgatcg tcgagatcct
    27301 cggcgcgctc ggcctgcccg cagcggagct gatggcgcgc gcgcaggcgg atgccaacaa
    27361 gctcgcgctg cgctgccaga ccgagcgcgc agccgaactg ggcctgttcg gggcgccgag
    27421 cttccgtgtc ggtgaggagt tgttctgggg caacgatcgc ctggaggatg cgctcgcctg
    27481 ggcgtgccgt gccgatcgcc ggtaggcgcg acgcgagtcg cgagcgggcg gttcgacggc
    27541 tgcggcgccg gtccgggggc cgtatcgcga cttgcgtcgc tcctaccggg gtttcgggat
    27601 ccgcgtcggt ttcttgtggg agcgacgcga gtcgcgagcg ggcggttcga cggctgcggt
    27661 gccggtccgg gggccgtatc gcgacttgcg tcgctcctgg cggggtttct ggatccgtgt
    27721 cggtttcttg tgggagcgac gcgagtcgcg atcgggcagt tcgacggctg cggtgccggt
    27781 ccgggggccg tatcgcgact tgcgtcgctc ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27901 nnnnnnnnnn nnnggggttt ctggatccgt gtcggtttct tgtgggagcg acgcgagtcg
    27961 cgatcggggc gcgagcacga cttgcgtcgc tcccgccggc gcctcgccga gggccctcga
    28021 ttgcgggctt gcggcaggtc ttacttcttc cccgcctgct cctgctcgtn nnnnnnnnnn
    28081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctcgattgcg ggcttgcggc aggtcttact
    28201 tcttccccgc ctgctcctgc tcgtactcgc gcaggcgctt ttcgtagcgc gcgcggtctt
    28261 cctcggcctg gcgggcgcgg cgggcggcgg cgtcttcggc atcgcggcgc gcagacgcgc
    28321 gctcggcgtc ctgggccgca cgctggcggg ccgtggcggc ttcggcctcg cggctggcct
    28381 cgacgcgctg ggcgcggatg cgctcggctt cggtagatgg cgcgatcgtc gcgtccccgc
    28441 tcggcccgga agggaccagg ggggaggcgg cgggcggggc ggcaggtggg gcaggggggc
    28501 tggggcgttc gccggcggcc ggcttggccg ctgctgcgcg ttggcgctcc gccagggtga
    28561 tgccgcggct ttcggcttcg agctcgcggg cacgccggat cgtctccagg cgctgcttct
    28621 ttgcctggtc gatgcaacgg ttgacgagga acttctcgta acagcccggc acggtggcct
    28681 ggtaggtggc ctcggcttcg gcgcgcagcg ccttgccatc ggctgcgagc gcctccgcgc
    28741 gggtacgctc ggcggcgcgg atggcctcgt ccgcggtctg cgcgtgggcg ggcaggatcg
    28801 tggaggcgag tagcgccgcg cacaggccgg gcatgatgga gcggtgggcg acgacgagag
    28861 acagggagcg gatacggatc atgcgtgcgg tttccggatt cgggtggccg agcggggcga
    28921 tcgaagacat tcatcgcgtt gccgcggccg agtggtcggc tcgggtttcc ggaatgcgtg
    28981 ttccggcttc gatcgagccg gcgcgtagaa tagcgccttt gcaaccgccc gcggcctgcc
    29041 ggccgtgtat ccgttcgtga tcagtcttcg taacttgcgc ctcgcgcgcg gcgccaaggt
    29101 cctcatcgag agcgccacgc tgcagatcca ccccggctgg aaggtcggcc tcaccggcgc
    29161 caacggcagc ggcaaatcca gcctgttcgc catgctgcgc gaccagctcc accccgatca
    29221 gggcgacctc gacctgccgc cgggctgggt gatcgcccac gtcgcgcagg aaacccccgg
    29281 gctggcgcag ccggcgatcg actacgtgct cgacggcgac gccgagctgc gccgcatcga
    29341 ggccgagctg gccgcagccg aagccgcgca cgatggctcg cacatcgggg aactccacgc
    29401 ccgcctgcac gagatcggcg gctatgcggc gcgcgcgcgc gcggcggccc tgctcgacgg
    29461 cctgggcttc ctgcagtcgg acatcgagcg ccccgtggcg gacttctccg gcggctggcg
    29521 catgcgcctg aacctggcgc aggcgctgat gtgccgttcc gacctgctgc tgctcgacga
    29581 gcccaccaac cacctcgacc tcgacgcggt gatctggctc gaggcctggc tgcgcgacta
    29641 ccgcggcacg ctgctgctga tctcgcacga ccgcgagttc ctcgacgcct gcgtgaccca
    29701 tatcgcccac atcgagcagc agaagctcgg cctctacacc ggcggctatt ccgacttcga
    29761 gcgtcagcgc ggcgaacgcc tcatccagca gcaggcgatg ttcgagaggc agcagcgcga
    29821 catcgcgcac atggaagact acatccgccg cttccgcgcc aaggccacca aggcccgcca
    29881 ggcgcagagc cggatcaagg cgctcgagcg catggagcgc atcgccgcgg cacacgtcga
    29941 cacgccgttc agcttcgcct tccgcgatgc gccgccggcg cccgacccct tgctgcagat
    30001 cgaggacggc gcggtgggtt acggcgacaa gacgatcctg gagcgcatca cgctgaccct
    30061 gcgcccgggc gagcgcgtcg gcctgctcgg gcgcaacggt gccggcaagt cgacgctgat
    30121 caagctgctc gcgcacgagc tgcagctcgc cagcggccgc cgcctggaag gcaagggcct
    30181 ggcgaccggc tacttcgccc agcatcaact ggaaacgctg cgcccggacg agtcggcgct
    30241 gcagcacatg gtcaggctcg atccgcagac gcgcgagcag gagctgcgcg actatctcgg
    30301 cggcttcgac ttccgcggcg acggcgtggg cggcacgtcc accccggcga ccacgccctg
    30361 cgggccgttc tcgggcggcg agaagtcgcg cctggcgctg gcgctgctga tctggcagcg
    30421 ccccaacctg ctgctgctcg atgagcccac caaccatctc gatctcgaga tgcgccacgc
    30481 gctgaccatg gcgctgcagg actacgaggg cgcgatggtg ctggtgtcgc acgaccgcgc
    30541 gctgctgcgc gccacctgcg atcgtttcgt gctggtcgat ggcggccgca tccagccctt
    30601 cgacggcgac ctcgacgact accgcgactg gctcgccagc cgccgcgccg aagttgccgc
    30661 ggccgctgcc tgccccgacc aggctgccga caaggccgcg cgcaaggccg accgcgccca
    30721 ggccgccgcc gaccgccagg cgcgcctggt ggcgcgtcgg ccgctggtga aggagctcga
    30781 gcagatcgac aagcgcatgg cggcgtggaa caaggagaag gccgagctcg acgcgaagct
    30841 cgccgatccg gcgctgtaca ccggcccgca ggcgggcgag gtgccggcgc tcaacaagcg
    30901 tcaggccgag ctcgccgagc gcatcgagga ggccgagctg cgctggctgg agctgcacga
    30961 ggcgctggag gcgatcccgg cggattgagg gggccgaacc cccttcgcgc cgggcggcgc
    31021 tcctgcgcag gacgtcgagc gtgcaggcct acttcggtaa ccagtgcgcc gggttcaact
    31081 tccaggtggc gaagtcctca tggccgacga tgcggtcctg cgcgtggcgc aggtcgaggt
    31141 cttgcggtgt cagtgcgtag ccatcggtgt gaaacatcac cgtgaccttc ggcgcgatca
    31201 gtttgtcggg gttctgcgcc tgcacgaccg caaggcgctt gctttcgagc cgcaccagcg
    31261 aaccggccgg atagatcccg accgcgcgga tgaaggcgtg cacgagttcg ggcttgaagt
    31321 ggaacttgct ccagtccagc agcttgcgga gcgcctcggt cggcggcatg cccttgtgat
    31381 agacgcggtt ggacgtaatg gcgtcgtaga catccacgat ggcgcccatc tggccataaa
    31441 ggctgatcgc gtcccccgcg agcttgttcg ggtagccggt accgtcgaag cgctcgtgat
    31501 gctgggcggc gacgtcgagc gcgatggcgc tgatgccggg tgtggcctgc aggatgatct
    31561 tgctctgcac gacgtggttc ttcatcttcg cgaactcggc gtcggtgagc ttggccggct
    31621 tgttcaggat cgcgtcgggg acgctggcct tgccgacgtc gtgcagcagt gcgcccgccg
    31681 caatttcgtg gatgaccggg cggggcagct gcaggttgcg ggcgaacgtg gtcatcagcg
    31741 cgcagaccga caccgagtgc tggaaggtgt aggcgtcgtg ctgcttgagc cgggcgagcg
    31801 ggatcagcgc gtcctgctgg gtgaagatcg agtcgacgat gcgttcgacg agcggctcga
    31861 tcagctccat ctcgatctgt ttgcccaggc gcacgtcggc catcatgtcg cggacgatga
    31921 gattggcctc gcggtgcagc gcgcgcgcgc gctcgatctc gcacgccacc tcctgcgctg
    31981 caatgcgcgg ggcggcctct gcggcgatgg cgtcgatctt ctcgtcgacc tcgtgctcga
    32041 tttcttcccg ggtcggtgct tcggggaggt cgagaccgcg cgcggtgtcg atgtagatct
    32101 cgtgcgtgcc gatcgcacgc actttccgca cgaccgcttc gtccttgacg gcgaagcggc
    32161 tgcgaacgaa gttgtgctcc atccagctgc agttcaggtc gtggacgtac atccccggct
    32221 ggagtttttc gatggggatg agcttgatca tggtcggtca gcggatgtga tgcgacatta
    32281 tcggcgatgc gctggagcgg gaaacgcgct tgcgtcgccc ggatcgagaa atacgccacg
    32341 caggacgatg caaatgggcc gtcccgccct gcgacgtgtc tgccaacggg gtatggatgc
    32401 gccgccctgc tagaatcctg gtctttccga caaactgccc tcaggagtcc gcggtgcgta
    32461 tcgtctgtct cgacctcgaa ggtgtgctcg tccccgaaat ctggatcgaa ttcgccgagc
    32521 gtaccggcat ccccgagctg cgccgcacca cccgcgacga gccgaactac gacaccctga
    32581 tgaggtaccg cctggacatc ctcgcgcaga agaagctcgg cctgcccgac atccaggacg
    32641 tgatcgccag catggggccg atgccgggcg cgcgcgagtt cctcgacgag ttgcgcgacg
    32701 cctaccaggt ggtgatcctg tccgacacct tctacgagtt cgccaagccg ttgatgaagc
    32761 agctcggcat gccgacgctg ttctgccaca gcctggaggc cgacgccggg ggcatcctgg
    32821 tcgactacca tctgcgcatg cccgaccaga agcgcgaggc ggtcaggcgc ttcaaggagc
    32881 tcaacttcaa ggtggccgcc gcgggcgact cctacaacga caccgcgatg ctcggcgaag
    32941 cccacggcgg catcctcttc cacccgcccg a
//
LOCUS       DAOQBS010000021        32030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_798_length_32030_cov_15.502392, whole genome shotgun sequence.
ACCESSION   DAOQBS010000021 DAOQBS010000000
VERSION     DAOQBS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 32030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32030
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_798_length_32030_cov_15.502392"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..544)
                     /locus_tag="PKN25_04275"
     CDS             complement(194..544)
                     /locus_tag="PKN25_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNV90146.1"
                     /translation="MSKSHVQIVSDVSKALATLRKEIPETMQGFGAMAKSSLQDGVLS
                     ELQKELIALAIAVTQRCDACIGFHIKALIRLGATREQIMETLGVCTYMGGGPALMYAA
                     EAVRAYEEMLPQAR"
     gene            complement(671..1480)
                     /gene="folE2"
                     /locus_tag="PKN25_04280"
     CDS             complement(671..1480)
                     /gene="folE2"
                     /locus_tag="PKN25_04280"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNV90147.1"
                     /translation="MNTPNAIAIPDVQSSADSRRIAIDKVGIKSIRHPIKVADKRGGV
                     QHTVASFNMYVGLPHNFKGTHMSRFIEILNGREREISVENFEPMLRTMVERLEAETGH
                     VEMSFPYFINKTAPVSGVQSLMDYEVCFIGSIGEGGAYEFTMKVVVPVTSLCPCSKKI
                     SAYGAHNQRSHVTVTAVLNDHLWIEEMVQMVEAQASCEVFGLLKRPDEKWVTERAYDN
                     PKFVEDMVRDVAGQLNREPRIDAYVVESENFESIHNHSAYALIERDKRGGA"
     gene            complement(1595..3463)
                     /gene="dxs"
                     /locus_tag="PKN25_04285"
     CDS             complement(1595..3463)
                     /gene="dxs"
                     /locus_tag="PKN25_04285"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNV90148.1"
                     /translation="MAPYPNLERVGCPADVRALDRRELGALASELRAFLIESVSKTGG
                     HLSSNLGTVELTIALHHVFNTPHDRIVWDVGHQTYGHKVLTGRREAMTGLRHFGGISG
                     FPRRCESEYDTFGTAHSSTSISAALGMAVAARARGEDRHAIAVIGDGAMSAGMAFEAL
                     NNAGDMENLNLLVILNDNEMSISPPVGALTKILARMMSGSTYNTARRVGEKVLGVAPP
                     VAELARKVEEYAKGMIGPGTLFEEFGFHYYGPIDGHDLDALIPTLQNLRKLKGPQFLH
                     VITRKGQGYKLAEADPVLYHGVSKFDHTAGIQTGKGGGKLTYTQVFGDWLCDMAKADP
                     RIVGITPAMREGSGLVRFAQEFPERYFDVGIAEQHAVTFAAGLACEGFVPVVAIYSTF
                     LQRAYDQLIHDVALQNLPVVFAIDRGGLVGADGATHHGAFDLSFLSCVPNMVVMAPSD
                     EDECRQMLYTAVQHAGPSAVRYPRGGGNGTAPRAQMRALPIGKGEVVRRGRGIALLAF
                     GSLLGVAREVAETLDATVANMRFVKPLDVELVAELATSHDLIVTLEENAVIGGAGAEV
                     SRLVDGLATRPRVLRLGLPDHFIDHGDQAQLLASVGLDQAGILASIARATGAPERA"
     gene            complement(3512..4420)
                     /locus_tag="PKN25_04290"
     CDS             complement(3512..4420)
                     /locus_tag="PKN25_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNV90149.1"
                     /translation="MNTDSKLPSFSEWTAQIQQRTEVALAGVLPDAAIAPQRLHEAMR
                     YAVLGGGKRVRPLLAHAAGALAGAAAEDLDRIACAVELIHAYSLVHDDLPCMDDDVLR
                     RGKPTVHVEFDEATALLAGDALQTLAFQVLAERPLGTDPANQLAMLNQLAIASGSRGM
                     AGGQAIDLASVGKRLGREELEFMHIHKTGALIRAAVLLGARAGGTLGADEHARLDHYA
                     KVVGLLFQVVDDLLDAEADTATLGKTAGKDADNDKPTYVSLLGLVDARRLAEEMLDEA
                     RASLRPFGARALRLEQLADYIVHRAF"
     gene            complement(4417..4671)
                     /locus_tag="PKN25_04295"
     CDS             complement(4417..4671)
                     /locus_tag="PKN25_04295"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNV90150.1"
                     /translation="MPKSASSPKSFEAAVSELEAIVQEMESGNLPLEDALARYQRGVG
                     LLRHCQTTLSDAEQRVRMLEGDGLTDFPTEPTRDGDEAGA"
     gene            4798..5904
                     /locus_tag="PKN25_04300"
     CDS             4798..5904
                     /locus_tag="PKN25_04300"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNV90151.1"
                     /translation="MSDIASKARLAQAASQLPVSWYFDQQVFELEKKLLFDAGPGYVG
                     HELMVPEVGNYRSLEWLDHAKLLFRTEAGVHQMSNVCRHRQAIMLQGSGTTKLVVCPV
                     HRWTYDRQGNLLGAPHFPEKPCLGLRRDELERWNGLLFKGPRSASADLAGMQVAGEFD
                     FSGYKLDKVEVHHCNYNWKTFIEVYLEDYHVVPFHPGLGNFVTCKDLSWQFGDWYSVQ
                     KVGITSLAKPGSETYAKWHKAVIDYYGEKKPTHGAIWLTYYPNVMVEWYPHVLVVSTL
                     IPTDVDKTTNVVEFYYPEDIVEFEREFVEAEQAAYMETAIEDDEIGERMDRGRRALLK
                     EGRNEVGPYQSPFEDGMQHFHEFYRRIMEPHIGG"
     gene            complement(6100..7038)
                     /gene="ispH"
                     /locus_tag="PKN25_04305"
                     /gene_synonym="lytB"
     CDS             complement(6100..7038)
                     /gene="ispH"
                     /locus_tag="PKN25_04305"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNV90152.1"
                     /translation="MNDKEILLANPRGFCAGVERAIEIVERALKRYGAPIYVRHEVVH
                     NKFVVDDLRAKGAIFVDELDEVPTGNTVIFSAHGVSLAVREEAERRGLRVFDATCPLV
                     TKVHVEVARMRDQGKEIVMIGHKGHPEVEGTMGQVDGGIHLVETPADVAGLQVADPEQ
                     LAYVTQTTLSVDDAAAIVDALRTRFPRVVGPKKDDICYATQNRQDAVKFMTPYADVVF
                     VVGSKNSSNSNRLREVAALRGVPAYLIDNAAGIDPAWIEGKRRIGVTAGASAPEVLVA
                     EVIARLEELSGGAVRNLDGVPERVTFALPKELQDPR"
     gene            complement(7071..7499)
                     /locus_tag="PKN25_04310"
     CDS             complement(7071..7499)
                     /locus_tag="PKN25_04310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNV90153.1"
                     /translation="MSEAIQANSLVTLHYRISLPSGQPLISTFNATPATLQLGAGEML
                     PAMERLLIGLMPGTHHIFELAADEAFGPYRDELVERVKREHMPDEEIEAMSIMEFTAP
                     DGSRYSGLVREIDAQSALIDFNHPLAGKAIRFEVQVIGIS"
     gene            complement(7496..7984)
                     /gene="lspA"
                     /locus_tag="PKN25_04315"
     CDS             complement(7496..7984)
                     /gene="lspA"
                     /locus_tag="PKN25_04315"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNV90154.1"
                     /translation="MLPWLILAVVVGVLDQVTKQMVMANLRLGELIPITGFFDLVLVF
                     NTGAAFSFLADHSGWQRWFFTILAFGISAWLLTLMHHHRHEKLLPTAFALIIGGALGN
                     VYDRLVHGAVVDFLHFHYAGWSWPAFNLADSAITVGVAMMLWGQFFGPKAGDPPRPGA
                     PS"
     gene            complement(8046..10874)
                     /gene="ileS"
                     /locus_tag="PKN25_04320"
     CDS             complement(8046..10874)
                     /gene="ileS"
                     /locus_tag="PKN25_04320"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNV90155.1"
                     /translation="MADYRKTLNLPDTPFPMRGDLPRREPNWIADWQQKKLYQRIRKA
                     SAGRPKFVLHDGPPYANGSLHIGHALNKILKDIIVRSKTLAGFDAPYVPGWDCHGLPI
                     EHKVEVTHGKNLPADRVRELCRAYAAEQIEIQKADFIRLGVLGDWDNPYRTMDFANEA
                     NEIRALAEMTKNGYVFKGLKPVNWCFDCGSALAEAEVEYADKPSPTIDVAFPVSDADA
                     GTHLRKLAAAFGLARLDKPAAAVIWTTTPWTIPANQALNAHPEFDYALVDVGDRLLVL
                     ARELVESALQRFQLAGTIIATAKGAALERIEFRHPFYDRVSPVYLAEYVGIDAGTGIV
                     HSSPAHGVDDFNSWHAYGRSNDEILSLVTGGGQYVSDLPFFGGMNIWKANPAIVDKLA
                     EVGALLSHGKITHSYMHCWRHKTPLVYRATAQWFVGMDRVAADGSTLRERALRAVEAT
                     KFYPAWGQARLHAMIANRPDWCISRQRNWGVPIPFFLHKETGELHPRTVELMEEVALR
                     VEQEGIEAWFKLDAAELLGAEAAQYEKISDTLDVWFDSGTTHKHVLRGSHNDGHPEGP
                     RADMYLEGSDQHRGWFHSSLLTGCAIDGHAPYRSLLTHGFAVDGAGRKMSKSLGNVVV
                     PQEVTGKLGAEILRLWVASTDYSGELSISKEILDRVVEVYRRVRNTLRFLLANTADFD
                     IATDAVPLEQWLDIDRYALAFTRQLAQQAEADYAKMEFHRVVQALQVFCAEDLGAFYL
                     DILKDRLYTTAAGSLPRRAAQTALWHITQTLLKLMAPILSFTAEEAWTVIHPGKDEGT
                     QDSVMLHTFHALPAQEGEAGLIARWETIRAVRAEGLKVIEALRTEGKVGASLQAELEL
                     ALTADKFAAMASLGEDLRFVTMTSAATLSEVARAEDERVVATPSAAQKCERCWHYVDT
                     VGSDAEHPTLCGRCASNLFGEGETRAHA"
     gene            complement(10919..11857)
                     /locus_tag="PKN25_04325"
     CDS             complement(10919..11857)
                     /locus_tag="PKN25_04325"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNV90156.1"
                     /translation="MQVHRGIPEQADTAAVLTIGNFDGVHLGHQALLSLLTDKARALG
                     LPAVVLTFEPHPREYFSPADAPARLASLREKLLLLSAAGVDRCHVCRFDARFAAQPAQ
                     AFIDDILVRGLGVRHLFIGDDFRFGARRQGNFAMLRAAGAEGGFGVESMPTLAVAGER
                     VSSSAVRAALAEGDLAHAARLLGRPYSIAGRVSHGDKLGRQLGFPTANIQMKHRRPAL
                     GGVYVVSVEGLASGPVAGVANIGLRPTATANGRARLEVHLFDWTQDCYGAHIRVHFLH
                     RLRAEQKFPSLDALRAQIALDSQTARDWLAANPLPA"
     gene            complement(11951..13348)
                     /locus_tag="PKN25_04330"
     CDS             complement(11951..13348)
                     /locus_tag="PKN25_04330"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV90157.1"
                     /translation="MRDRTGESFAGRGPGARDDLSLGQTPGLAAAAEAFAAGRIDRRG
                     LLKFAGASLAMLVSPVGLASSASLLAVRVWPSAEYTRITLEGSSRLRHSHMLVEDPQR
                     LVVDLEGVQLDSVLQSLPSKVLDSDPYIRLIRAGQNRPGVVRVVIELKAAINPQVFTL
                     DPVGNYGHRLVLDLHPTEAHDPLMALIMKDSPMDAAMGDAGGNTTAVAREEPREPVRR
                     GKRNEPAVDRLYTVVLDAGHGGEDPGAIGRGGSYEKDVTLSIAQRLKRKIDAMPGMRA
                     VLTRDGDYFVPLHQRVARARRVRADLFVSIHADAFVRPEANGSSVYVLSERGASSSAA
                     SWLAQKENDADLVGGVNLARQDGHIARTLLDLSQTATINDSFKLGRAMLGELGTINRL
                     HKPEVEQAGFAVLRAPDIPSVLVETAFISNPQEERRLNDEAYQDKMAMALMRGVKRYF
                     EEHAPSGPTRVARLD"
     gene            complement(13288..13818)
                     /gene="tsaE"
                     /locus_tag="PKN25_04335"
     CDS             complement(13288..13818)
                     /gene="tsaE"
                     /locus_tag="PKN25_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNV90158.1"
                     /translation="MIESIQTADDNGARLQAHLPDEAATVALGGALAGVLAPGLQIWL
                     QGNLGTGKTTLTRGLLRALGHEGKVKSPTYTLIEPYVVSSLNLYHFDFYRFTSPDEYL
                     DAGLDEYFAGEGVCIVEWPDKASPHLPSPDVEIVLQAGESGRDVAISANTEAGRTCVI
                     GLVKALRGADPAPGTT"
     gene            13834..14934
                     /gene="queG"
                     /locus_tag="PKN25_04340"
     CDS             13834..14934
                     /gene="queG"
                     /locus_tag="PKN25_04340"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNV90159.1"
                     /translation="METKLPTPDGAAVDGTVLDGAALVARIRQWGRELGFDAVGVGGV
                     DLADAEPGLVAWLEAGFHGDMDYMVRHGMKRARAAELLPGSVRVISVRMGYWPDAAPA
                     MDVLGDPERAYVSRYALGRDYHKLVRNRLQKLADRISAEVPHQYRVFTDSAPILEVEH
                     ASRKGLGWRGKHTLLLDRTAGSWFFLGEILTDLPLPVDAPVASHCGRCTACIDACPTG
                     AIVAPYRVDARRCISYLTIELHGAIPEELRPLLGNRIYGCDDCQLVCPWNRFAQLGRE
                     PDFAPRQGLDDARLAELFAWTAAEFSERTAGSPIHRIGHARWLRNIAVALGNGPATPA
                     ARAALQARADDEDAVVREHVAWALARLAAAAG"
     gene            14971..15171
                     /locus_tag="PKN25_04345"
     CDS             14971..15171
                     /locus_tag="PKN25_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNV90160.1"
                     /translation="MEERIVVLETKLMAAEDLLDELNRTVWRQQQELDLLRQHLHQLA
                     QQVKTLQPGDPLRPEDEIPPHW"
     gene            complement(15220..15513)
                     /locus_tag="PKN25_04350"
     CDS             complement(15220..15513)
                     /locus_tag="PKN25_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNV90161.1"
                     /translation="MSPERKIDLAWLMLVLLSVGGAGIARSDANAVLVAVGVSLVMAI
                     KLRLVCNYFLELHEAHPRIRRAVLAFCHGMPILVILTTAFGDTLSSLTGALLG"
     gene            complement(15524..16051)
                     /locus_tag="PKN25_04355"
     CDS             complement(15524..16051)
                     /locus_tag="PKN25_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HNV90162.1"
                     /translation="MWVGIFCEITEFALLFAVFFIARAHHPEAFQAGPERLSLLAGTG
                     YTLMLLTSGWCVARALHAMRRGDRRATVRWMLAGFVFGLGYPAIKIFELQRNFALGLD
                     GSAGVFIVSYYYLTFTHLVHVFWGLLGMMWVIARTGLGAYTPEEHSGLEAFACYWHVT
                     DMIWLMIFPLFYLLG"
     gene            16372..17820
                     /locus_tag="PKN25_04360"
     CDS             16372..17820
                     /locus_tag="PKN25_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNV90163.1"
                     /translation="MAKVKFFGEESIPLEMHKVRIVQKLNLPPVERRLEAITEAGNNT
                     FLLRNADVFMDMLTDSGVNAMSDQQLAAMMIADDSYAGSATYTRLENKLHEIFGMPFF
                     LPAHQGRACENILAQVLVSPGSVVPMNYHFTTTKAHITLNGGKVEELVADVGLEVVSV
                     HPFKGNMDVAKLRDVIGLHGADKIAFVRMEAGTNLIGGQPFSVQNLADIRRVCDEFGL
                     MLVMDASLLADNLHFIKEREEACKGLSIRDITRRMADLCDVIYFSARKLGCARGGGMC
                     IRSEELYRRMRGLVPLYEGFLTYGGMSVREMEALTVGLEETMDEEVINQGPQFIGFMV
                     DELVKRGIPVITPAGGLGCHINAMQFVDHIPQAQYPAGALASALYLAGGIRGMERGTL
                     SEQREPDGSELFANMELVRLALPRRVFTLSQVKYAVDRLEWLYANRRLIGGLVFTEEP
                     EILRFFYGRLAPVSDWQNELVAQFRKDFGDSL"
     gene            17973..18491
                     /locus_tag="PKN25_04365"
     CDS             17973..18491
                     /locus_tag="PKN25_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HNV90164.1"
                     /translation="MLKSHAVLESRRPSLSASVAAAVLALGVALPGVAVAETVGHVDT
                     AFKLIGRNHQVVVEAFDDPSVKGVACYVSRARTGGLKGTFGLAEDTSDASVACRQVGD
                     ISFSGPLREQEEVFSQSASILFKKVRIVRMVDPKRNTLVYLVYSDKLIEGSPQNSVTA
                     VPVPAALAIPLR"
     gene            complement(18592..18969)
                     /locus_tag="PKN25_04370"
     CDS             complement(18592..18969)
                     /locus_tag="PKN25_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV90165.1"
                     /translation="MDQLAAQPATDAPLVVLALCAQWCGTCREFRPTLERLAEARRDV
                     VFAWADVEDDAELVGDLDVENFPTLALFRAGVALHYGVSLPHESVVARLVETFAAAAP
                     RAAPGLAAEIGALARRLSQPGGG"
     gene            complement(19186..19455)
                     /locus_tag="PKN25_04375"
     CDS             complement(19186..19455)
                     /locus_tag="PKN25_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90166.1"
                     /translation="MYPRSLTVLEGRRKAAGARSALDTAERAIRHAIGAGFRIGCRVL
                     VGRVPGSVIGYNIASSGRFGGAAYPLLVETEFGIAKCSMQEVCPA"
     gene            complement(19568..21535)
                     /locus_tag="PKN25_04380"
     CDS             complement(19568..21535)
                     /locus_tag="PKN25_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNV90167.1"
                     /translation="MSIDRHTLELLAPAKTADFGIEAIDHGADAVYIGGPAFGARSSA
                     DNSVEDIARLVQHAHRYHAEVFVATNTILFDHEIEPARKLIWQLYDAGVDALIVQDMG
                     LLELDLPPIQLHASTQTDIRDASKARFLQDVGFSQIVLARELSLNEVKKIAAATTCQL
                     EYFVHGALCVAFSGQCYISHAHTGRSANRGECSQACRLPYDLKDKDGHTVASNQHMLS
                     MKDNNQSANLRALAAAGVSSFKIEGRYKDLSYVKNITAHYRTLIDEILEHPDTDGARY
                     RRASSGRTTFFFTPQADKTFNRGYTDYFTNDRRHGIEAFESPKFVGEPIGRVKKIDTK
                     GRKFFDVERAAPIHNADGLTWYDPKGELTGLRVNRAEADGGGEGIDRIFPADPLPTDL
                     VPGTSLFRNHDHEFERALGKKSAERRIRVDARFAATHDGFALTLTDEDGVAVTATLAA
                     AFEPAQNAERALATLREHLGKLGNTIFSAGELVLDLPAAPFLPAGQLNALRRDAVERL
                     EAGRLAAHARPLRAAPVEPPVPYPQDALSYLANVSNDKARAFYARHGVKLIDAAYEAN
                     EERDDVSLMITKHCLRYSFNLCPKEVKGIRPDPMQLVNGDETLTLKFDCKRCEMHVIG
                     ALRPHVAKMRDTVVAHKVSFVPQRKTNASVR"
     gene            complement(21673..22551)
                     /locus_tag="PKN25_04385"
     CDS             complement(21673..22551)
                     /locus_tag="PKN25_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90168.1"
                     /translation="MLHHPRIALVTGATMAKPDPETHLLVDALAQRGIVADVPPWNSE
                     QDWAAYPLVVVRTPWDYFRHLDAFLAWAERTSALTHLVNPVAVIAWNSHKGYLRELAA
                     GGVATVPTLWIERGDTAAGARLTAQGWDEVVVKPAVSIGAIGALRARADDPACRAHLE
                     DLVAAGDVMVQPFVHSIAEAGEVSMIYFGGVFSHAICKRPAAGDYRVQDLYGGTVHAH
                     TPTAAERRLAEAVLAQAPAATTYARVDLVSHEGRPALMELEAIEPELFLGATPDAAAN
                     FAAELARRLEVAQAQG"
     gene            complement(22614..22970)
                     /locus_tag="PKN25_04390"
     CDS             complement(22614..22970)
                     /locus_tag="PKN25_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90169.1"
                     /translation="MPTSVRTLLRALFIAALGGAFWLALMPIEPVIKLFSWQDKVEHA
                     LLFAGLAILGVAAWPQRVGLLAAGLLAYGAAMEVAQSTTAYRVGDPLDWLADAVGLLV
                     LLPFLRNRKTNTANAR"
     gene            23133..24311
                     /locus_tag="PKN25_04395"
     CDS             23133..24311
                     /locus_tag="PKN25_04395"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNV90170.1"
                     /translation="MQDGKVRELSSSRTRAALRVALLAGMLAAGSMHAPLAGATGMVY
                     PSETGPLRVTEVARGLESPWALAFLPDGRMLVTERPGRMRIVDTAGRVSEPIAGVPEV
                     HARGQGGLLDVALAPDFDRDRLIVFSYAEPTARGARTAVARARLDVDGLRLEDVRRIF
                     AQDEDPAGNHHWGSRLVFGRDGRLFVTLGDRFHHRERAQALDSHLGKVVRIDPDGGVP
                     ADNPLVGRAGVRGEIWSWGHRNVQGAALHPRTGELWTHEHGPQGGDEINRTLGGRNYG
                     WPEITYGREYVTGRKIGAGSEREDVAAPVLQWTPSIAPSGMAFYTGDAFPQWQGNLFV
                     GALKFQLLARLVLDGERVVREERLLEGLGRIRDVRQGPDGRLWLLDESAGRVLRIDPQ
                     "
     gene            complement(24360..26735)
                     /locus_tag="PKN25_04400"
     CDS             complement(24360..26735)
                     /locus_tag="PKN25_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV90171.1"
                     /translation="MTRTIGRFEIRRELGRGAQSVVHLAWDPQLEREVALKALHLGPR
                     PGHDNASLLAEARAVSRLRHPGIVPVFEAGEDGGDPYLVFEYVPGESLAALLDRRGPL
                     PAGEAVERMLEILAALAEAHAQGVIHRDLKPSNVLLDGGGHARVMDFGIARRIEAAGA
                     SEGLSGTPGYMAPEYIERREIGPRNDVFAAGVLLIELLAGRPLAREGGVKRVLERTRS
                     TPVTLPADGPPVDEALAAIALRAAALDPLARFADAAEFRLALEAWRQPAETDPEAGSE
                     ARPGAVDFLMRRMKHRGDFPALSESVVAINRIAASESESVGTLSALILRDFGLTNKLL
                     RVVNSAHFRPAGGGRISTISRAVVVLGFDAVRNIAITVLLFEHLQNKSNASQLQEEFL
                     RACLAGLFARELARRLRLRDGEQSYICAVFHNLGRLLCQFYFPEESEDIRRVVQQHGC
                     SEESAAQRVLGAGYEELGIALARAWGFPELIQNSMRTLPEGPVHAPRSADERLQSLAA
                     CANACCDAVSRLAPAERERALADIAARFDEAVPVPAREAREHLRSAVDEIGEFARVVK
                     VSLAQTRFGRNLRHFIDGSTPAEGARVDATLMPASALLEQSLPAEGGAEGQRAADPQA
                     VLSAGIQDISNSLVEDFRLNDVLRIILETMYRAMGFKRVLLCVRDARSNAMCGRFGFG
                     PEVGELAKQLRFSLSAQPDNVFNVATARGVDVLISDVDDPKIAARVPAWYRRSVAART
                     FVLFPLTIKGRPVAMIYADKDRAGELRISEQELAMLRTLRNQAVLAIKQAG"
     gene            26859..27224
                     /locus_tag="PKN25_04405"
     CDS             26859..27224
                     /locus_tag="PKN25_04405"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNV90172.1"
                     /translation="MESPFKGKTGLRRIFNAARYSMDGLRAAYRHEDAFRQEIWLTLV
                     AVPLALWLGEGAVERALMIGSVLLVLVVELLNSAVEATVDRVSLERHELAKRAKDVGS
                     AAVLLALLHAAVVWGLLLF"
     gene            complement(27255..27818)
                     /locus_tag="PKN25_04410"
     CDS             complement(27255..27818)
                     /locus_tag="PKN25_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNV90173.1"
                     /translation="MSKIVRNQVVPHRRLLLKGLATLPLGLSAAAQAAQRDPQLDFRH
                     THTDERLSVAFRNRQGYIQPALQRIDWLLRDFRTGESTRMDPRLYDMLHALSLACGGN
                     TFEIISGYRSPTTNTMLRKTRGGGVARRSLHMDGKAIDIRLVGVDTARLRDAALALGG
                     GGVGYYPDSDFVHIDTGPVRSWGPRSA"
     gene            27862..29511
                     /locus_tag="PKN25_04415"
     CDS             27862..29511
                     /locus_tag="PKN25_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNV90174.1"
                     /translation="MQSKPGLGKCFVTAAALAALALMASCVHATAAGEAAVVAVEPAA
                     SDSALALRIEQRLRERAATADDPIALFYLARAYRSVWTEPARVHALLAAVEAVRGHGL
                     DAADFAPARLRAGAVPAADPERAAERELLLTDTLAALLFQLRHGKVDPRALYREWNFT
                     PPPSPYERAAELARVLQASDLAAAVDAYAPDLPLYRALRAELLAQQGRLAVGDWPKVA
                     AGPTLKPGASSSRVASLRARLAAAGERVSEARDKSHYDEALVEAVKRFQAAHGLQADG
                     VLGAQTLEALNASPAQRVAQIRANLERLRWVASDLQGDRLLVDIVGYHADLVLDGQPV
                     WSSRVIVGKPKRRTPSLLDSVTHLVLNPKWVVPPTILREDVIPGAARNPSYLANRRLR
                     VVDRSGQTVDPATIDWSGARQSGFPYRVEQQSGADGSLGRIKFSLSNPYVIYLHDTNA
                     RSLFKRAERALSSGCVRVEKPEELAVLLLADSGRWSAQALQAALDSGRTRTVDVGRDV
                     KVLLHYATAALDEAGRVLLRNDIYGYDAAIVAALDAPAPAR"
     gene            29600..30826
                     /locus_tag="PKN25_04420"
     CDS             29600..30826
                     /locus_tag="PKN25_04420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNV90175.1"
                     /translation="MRTLDYTTEDLCRAPRLRIALVTETWAPEVNGVAMTLGRMVDGL
                     IRRGHGVQLIRPRQRPGETAAHGEGLEEVLARGLRLPRYDGLKLGLPARVRLVREWSR
                     QRPDLVHVATEGPLGWTAVTAANKLRIPVSSDFHTNFDHYSGHYGMGWLRQPVAAYLR
                     RFHNRSAVTFVPTAALAAQLSAQGYRSVEVISRGVDTALYSPARRDEALRRAWGLPPG
                     GLAVISVGRLAPEKNLGLAMRAFAAIRRLRPDARMVLVGDGPQRAALARAHPDAVFVG
                     MRHGEDLAAHYASADLFLFPSLTETFGNVTLEAMASGVCPVAYDYAAAAEVIRDLGNG
                     ASVACGDEEGFIARAVQMAGADALRAELARAARRSAEAIDWERVNDRFAAALLRVWRA
                     GSGRPLDLSEPRPEET"
     gene            30830..31684
                     /locus_tag="PKN25_04425"
     CDS             30830..31684
                     /locus_tag="PKN25_04425"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNV90176.1"
                     /translation="MSSVRAIFLSDVHLGTRACQAERLLDFLREHESDYLFLLGDIVD
                     FWAMKRSVQWAPSHNTVVQKVLRRARHGCQVIFIPGNHDEALREHAGVAFGDIRVESE
                     WIHHTADGRRYWLVHGDEYDQVTRHHRWVALLGDVAYNALVRINLLLSRARRLLGVPG
                     YWSLAGYAKQKVKKAVSFIFDFEDAVAHAAQQRGVDGVICGHIHWASDRRIGDIRYLN
                     CGDWVDSCSAVVEHHDGRIEVLQWGALGAHALAPLPALPAPRPASVRPQSAPVPAGAE
                     RIYLMPDI"
     gene            complement(31837..>32030)
                     /locus_tag="PKN25_04430"
     CDS             complement(31837..>32030)
                     /locus_tag="PKN25_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90177.1"
                     /translation="YRPQDRRFWLMIVLNLLSAILAWLLRSWPLVPLATVVVALFALG
                     NAALGMFIAWDLLRDERED"
BASE COUNT         4748 a        10639 c        11611 g         5032 t
ORIGIN      
        1 gcacctacgc ctgatttcga gtcaggtaca ttcgaccact ctgccacctc tccgctcgaa
       61 ggtcggcatt atagtgtttt tttccgggaa gtgaacagtt ttttgcgggg ggtaaagtga
      121 agagaccccg tcgccggccc cgacccggcg catcgggacg ggcaaggcgg ccgcggtcgg
      181 gcccgggctg cgctcacctg gcctgcggca gcatctcctc gtaggcgcgc accgcttccg
      241 cggcgtacat cagggccggg ccgccgccca tataggtgca cacgcccagg gtctccatga
      301 tctgctcgcg ggtcgcgccg aggcggatca gcgccttgat gtggaagccg atgcaggcgt
      361 cgcagcgctg ggtgacggcg atcgcgagtg cgatgagttc cttctgcagc tcgctcagca
      421 cgccgtcctg cagcgagctc ttggccatcg cgccgaagcc ctgcatggtc tcggggatct
      481 ccttgcgcag cgtggcgagc gccttggaga catcgctcac gatctggacg tgggacttgg
      541 acatcatgcg ttctcctatt tcagaaaacg cttatcttac tccgccgacg cgcttgcgac
      601 catcgcgtcg cggcgccggg cgtgcgtgcc cgagcgcacc cgcgacacgg ccccgctaaa
      661 ggggcggggg tcaggccccg ccgcgcttgt cgcgctcgat cagcgcgtaa gcggagtggt
      721 tgtggatcga ctcgaagttc tcggactcga ccacgtaggc gtcgatgcgc ggctcgcggt
      781 tcagctggcc ggcgacgtcg cgcaccatgt cctcgacgaa cttggggttg tcgtaggcgc
      841 gctcggtgac ccacttctcg tcggggcgct tgagcaggcc gaagacctcg cacgaggcct
      901 gggcctcgac catctgcacc atctcctcga tccacaggtg gtcgttgagc accgcggtga
      961 cggtgacgtg agagcgctgg ttgtgcgcgc cgtacgcgga gatctttttc gagcatgggc
     1021 acaggctggt caccggcacc acgaccttca tggtgaactc gtaggcgccg ccctcgccga
     1081 tgctgccgat gaagcagacc tcatagtcca tcaggctctg cacgccggac accggcgccg
     1141 tcttgttgat gaagtagggg aagctcatct cgacgtggcc ggtctcggcc tcgaggcgct
     1201 cgaccatggt gcgcagcatg ggctcgaagt tctccaccga gatctcgcgc tcgcggccgt
     1261 tgaggatctc gatgaagcgc gacatgtggg tgcccttgaa gttgtggggc agaccgacgt
     1321 acatgttgaa gctggccacc gtgtgctgca cgccgccgcg cttgtcggcg accttgatgg
     1381 ggtggcggat cgacttgatg ccgaccttgt cgatcgcgat ccggcggctg tcggcgctgc
     1441 tctgcacgtc cgggatggcg attgcgttgg gggtgttcat gggcaggttc cctgcgggtg
     1501 gtcgggggaa tgggcgtgcc ggagccgcca gcaaagtgca atcgtgttgc actttgctgg
     1561 cgatcttatc tttcccgaca aaaatgctca acctttaggc gcgctccggg gctccggtcg
     1621 cgcgtgcgat gctggcgagg atgccggcct gatcgagccc gaccgaggcg agcagttgtg
     1681 cttggtcgcc gtggtcgatg aagtggtcgg gcaggcccag gcgcagcacg cgtgggcgcg
     1741 tggcgaggcc gtcgacgagc cgtgagacct cggcgccggc gccgccgatc accgcgttct
     1801 cctcgagggt gacgatgagg tcgtggctgg tggcgagttc ggccaccagc tcgacgtcga
     1861 gcggcttgac gaagcgcatg ttggccaccg tggcgtcgag cgtctcggcg acctcgcgcg
     1921 ccactccgag caggctgccg aaggcgagca gggcgatgcc gcggccacgg cgcacgacct
     1981 cgcccttgcc gatcggcagg gcgcgcatct gtgcccgggg cgcggtgccg ttgccgccgc
     2041 cacgcggata gcgcaccgcg ctcgggcccg catgctgcac cgcggtgtag agcatctggc
     2101 ggcattcgtc ctcgtctgag ggcgccatca ccaccatgtt gggcacgcag ctgaggaagg
     2161 agaggtcgaa ggcgccatgg tgggtcgcgc cgtcggcgcc caccaggccg ccgcggtcga
     2221 tcgcgaacac gaccggcagg ttctgcagcg cgacgtcgtg gatgagctgg tcgtaggcgc
     2281 gctgaaggaa ggtcgagtag atcgccacca ccggcacgaa gccctcgcag gccagcccgg
     2341 cggcgaaggt gaccgcgtgc tgctcggcga tgccgacgtc gaagtagcgc tcggggaact
     2401 cctgcgcgaa gcgcaccagg cccgagccct cgcgcatcgc cggggtgatg ccgacgatgc
     2461 gcggatcggc cttggccatg tcgcacagcc agtcgccgaa cacctgggtg taggtgagct
     2521 tgccaccgcc cttgccggtc tggatgccgg cggtgtgatc gaacttcgac accccgtggt
     2581 agagcaccgg gtcggcctcg gcgagcttgt agccctggcc cttgcgggtg atcacgtgga
     2641 ggaactgcgg tcccttgagc ttgcgcaggt tctgcagggt cggaatcagt gcgtcgaggt
     2701 cgtggccgtc gatcggaccg tagtagtgga agccgaactc ctcgaacagc gtgcccgggc
     2761 cgatcatgcc cttggcgtac tcctcgacct tgcgcgcgag ctcggccacc ggcggggcga
     2821 cgccgagcac cttctcgccg acccggcgcg cggtgttgta ggtcgagccc gacatcatgc
     2881 gggcgaggat cttggtgagc gcccccaccg gtggcgaaat cgacatctcg ttgtcgttga
     2941 ggatcaccag caggttgagg ttctccatgt cgccggcgtt gttgagggcc tcgaaggcca
     3001 tgccggccga catcgcgccg tcgccgatca ccgcgatcgc gtggcggtcc tcgccgcgtg
     3061 cgcgcgccgc cacggccatg ccgagcgcag ccgagatcga ggtggaggag tgtgcggtgc
     3121 cgaaggtgtc gtactcgctc tcgcagcgcc gcgggaagcc ggagatgccg ccgaagtggc
     3181 gcagcccggt catcgcctcg cggcggccgg tgagcacctt gtggccgtag gtctggtggc
     3241 cgacgtccca cacgatgcgg tcgtgagggg tgttgaaaac gtggtgcagc gcgatcgtca
     3301 gctcgacggt gccgaggttg gaggacagat ggccgccggt cttcgatacc gactcgatca
     3361 ggaaggcgcg caattcggag gcgagggcgc cgagttcgcg gcgatcgagt gcacggacgt
     3421 cggcgggaca gccgacgcgt tccaggttcg ggtagggggc catgggggat ccggcttttg
     3481 ggttgttctt gggggccgca gtcaggcgcg atcagaaggc gcgatgcacg atgtagtccg
     3541 cgagctgctc caggcgcagc gcacgcgcgc cgaaagggcg caggctggcg cgcgcctcgt
     3601 cgagcatctc ctcggcgagg cggcgggcgt cgaccaggcc gagcaggctc acgtaggtcg
     3661 gcttgtcgtt gtcggcgtcc ttgcccgcgg tcttgcccag cgtggcggtg tcggcctcgg
     3721 cgtcgaggag gtcgtcgacc acctggaaga gcaggcccac gaccttggcg tagtggtcca
     3781 ggcgtgcgtg ctcgtcggca cccaatgtgc cgccggcacg cgcgccgagc agcaccgcgg
     3841 cgcggatcag cgccccggtc ttgtggatgt gcatgaactc gagctcttcg cgccccagcc
     3901 gcttgcccac cgaggcgagg tcgatcgcct ggccgccggc catgccgcgc gagcctgacg
     3961 cgatggcgag ctggttcagc atcgcgagct ggttcgcggg gtccgtgccg agcggacgct
     4021 cggcgagcac ctggaaggcc agcgtctgca gcgcgtcgcc ggcgagcagg gcggtggcct
     4081 cgtcgaactc gacatgcacg gtaggcttgc cgcgacgcag cacgtcatcg tccatgcaag
     4141 ggagatcgtc gtgcaccagc gaataggcgt ggatcagctc gaccgcgcag gcgatgcggt
     4201 cgaggtcctc ggcagcggcg ccggcgagtg cgccggccgc atgcgcgagc agcggacgca
     4261 cgcgcttgcc accgccgagc accgcgtagc gcatcgcctc gtgcaggcgc tgcggggcga
     4321 tcgcggcatc gggcagcacg ccggcgagcg cgacctcggt gcgctgctgg atctgcgccg
     4381 tccattcgga gaaggagggg agcttgctgt ctgtgttcat gcgcctgcct cgtcgccgtc
     4441 gcgggtcggt tcggtcggga aatcggtgag gccgtcgcct tcgagcatgc gcacgcgctg
     4501 ctcggcgtcg ctgagcgtgg tctggcagtg acgcagcagg ccgacgccac gctgatagcg
     4561 cgcgagggca tcctcgagcg gcagattgcc ggattccatt tcctggacaa tggcctcgag
     4621 ctcggagacc gccgcttcga acgacttggg ggaggatgcc gactttggca tggatcgata
     4681 tctggcgcgg caaaagcgga aaaataaccg aaggcgggta tggcggtcaa acggggggtg
     4741 tcttacacta aaccctttat ttttccggat ttcttgtccg caagaggagg ttcggggatg
     4801 tccgacatcg cttccaaggc tcgactggcc caggcagcct cccagctgcc tgtctcgtgg
     4861 tacttcgacc agcaggtttt cgaactggag aagaaactcc tcttcgatgc cggtccgggg
     4921 tacgtcggcc atgagctgat ggttcccgag gtcggcaact accgctcgct ggagtggctc
     4981 gaccacgcca agctgctgtt ccgcaccgag gcgggtgtgc accagatgtc caacgtctgc
     5041 cgccaccgcc aggcgatcat gctgcagggc agcggcacga ccaagctcgt ggtctgcccg
     5101 gtgcaccgct ggacctacga ccgccagggc aacctgctcg gcgcaccgca cttccccgag
     5161 aagccctgcc tgggcctcag gcgcgacgag ctcgaacgct ggaatggcct gcttttcaag
     5221 ggcccgcgct cggcgagcgc cgaccttgcc gggatgcagg tggcgggcga attcgatttc
     5281 tcgggctaca agctcgacaa ggtcgaggtc caccattgca attacaactg gaagaccttc
     5341 atcgaggtct atctggagga ctatcacgtc gtgcccttcc accccggact gggcaacttc
     5401 gtgacctgca aggacctgag ctggcagttc ggcgactggt attcggtaca gaaggtgggc
     5461 atcacctcgc tcgccaagcc gggctcggag acctacgcca agtggcacaa ggcggtgatc
     5521 gactactacg gcgagaagaa gccgacgcat ggtgcgatct ggctcaccta ctacccgaat
     5581 gtgatggtgg agtggtaccc gcacgtgctg gtggtgagca cgctgattcc gacggatgtg
     5641 gataagacga ccaacgtggt ggagttctac tacccggagg acatcgtcga gttcgagcgc
     5701 gagttcgtcg aggccgagca ggccgcctac atggagactg cgatcgagga cgacgagatc
     5761 ggcgagcgca tggatcgcgg ccggagggcg ctgctgaagg aggggcgcaa cgaggtcggc
     5821 ccctaccagt cgcccttcga ggacggcatg cagcatttcc acgagttcta ccggcgcatc
     5881 atggagccgc acatcggcgg gtgaagcgga gagaagcgag gcgcgacctg cggctcgcgc
     5941 attccatcac gcgcggcgcc gattcaaggc gccacgatcg cgacttgcgt cgctcccaca
     6001 ggaaagtaca cgcgactcga ccgccaagta cacgcgactc gaccgccgcc ttgcagaagc
     6061 gacgcaaggc gcgattctct cccgccttgc tccgaagctt cagcgcgggt cctgcaactc
     6121 cttgggcagc gcgaaggtca cgcgctcggg cacgccgtcg aggttgcgca ccgcgccgcc
     6181 gctcagttct tccaggcgcg cgatcacctc ggccaccagc acctcgggcg ccgaggcccc
     6241 cgcggtcacg ccgatgcggc gcttgccctc gatccaggcg gggtcgatgc cggcggcgtt
     6301 gtcgatcagg taggcgggca cgccgcgcag cgcggcgacc tcgcgcaggc ggttggagtt
     6361 ggagctgttc ttcgacccga ccacgaatac cacgtccgcg tacggggtca tgaacttcac
     6421 cgcgtcctgg cggttctgcg tggcgtagca gatatcgtcc ttcttcgggc cgacgacccg
     6481 ggggaagcgg gtgcgcaggg catcgacgat cgcggccgcg tcatccacgg acagcgtggt
     6541 ctgggtcacg taggcgagct gttctgggtc ggccacctgc aggccggcga cgtcggccgg
     6601 ggtctcgacc agatggatgc cgccgtcgac ctggcccatc gtgccctcga cctcggggtg
     6661 gcccttgtga ccgatcatga cgatctcctt gccctggtcg cgcatgcgcg ccacctcgac
     6721 atggaccttc gtcaccagcg ggcaggtggc gtcgaagacg cgcagcccgc ggcgctcggc
     6781 ttcctcgcgc accgcgagcg agacgccatg ggccgagaag atcaccgtat tgccggtagg
     6841 gacctcgtcg agttcgtcga cgaaaatcgc acccttggcg cgcaggtcgt ccacgacgaa
     6901 cttgttgtgc acgacctcgt ggcgcacgta gatcggcgcg ccgtagcgct tcagggcgcg
     6961 ttcgacgatc tcgatcgcgc gctcgacccc ggcgcagaag ccgcgcgggt tggcgagcag
     7021 gatttccttg tcgttcatct ttcgtctctg tggggtgtcg cgggcggcgc tcaggagatc
     7081 ccgatcacct gcacctcgaa gcggatcgcc ttgcccgcca gcgggtggtt gaagtcgatc
     7141 agcgccgact gcgcgtcgat ctcgcgcacc aggccggagt agcgtgagcc atcgggtgcg
     7201 gtgaactcca tgatgctcat cgcttcgatc tcctcgtcgg gcatgtgttc gcgcttgacg
     7261 cgctcgacca gctcgtcgcg ataggggccg aaggcttcgt ctgccgccag ttcgaagatg
     7321 tggtgggtgc ccggcatcag gccgatcagc aggcgctcca tcgccggcag catttcgccg
     7381 gcgcccaatt gcagcgtggc gggggtggcg ttgaaggtgc tgatcagcgg ctggccgctc
     7441 ggcagcgaga tgcggtagtg cagggtgacg aggctgttgg cctggatcgc ttcgctcaag
     7501 atggtgctcc cggtcggggc gggtcgccgg ctttggggcc aaagaactgg ccccagagca
     7561 tcatcgcgac acccacggtg atggccgagt cggccaggtt gaacgccggc cagctccagc
     7621 cggcgtagtg gaagtgcagg aagtcgacca ccgcgccatg cacgaggcgg tcatagacgt
     7681 tgccgagcgc accgccgatg atcagggcga aggcggtcgg cagcagcttt tcgtggcgat
     7741 ggtggtgcat cagcgtcagc agccaggcgc tgatgccgaa ggccagtatg gtgaaaaacc
     7801 agcgttgcca gccgctgtgg tcggcgagaa agctgaacgc ggcgcccgtg ttgaacacca
     7861 gcaccagatc gaagaagcct gtgatcggaa tcagctcgcc gaggcgcaga ttcgccatca
     7921 ccatctgctt ggtgacctgg tcgagcacgc cgaccaccac ggcgaggatc agccagggca
     7981 gcatcagaga cagcgcactg cgcgcgtgcg gcgtcgggta aggtgtcgtc atcgtgcgga
     8041 ctgcctcagg catgggcgcg ggtttcgcct tcgccgaaca ggttgctcgc acagcgcccg
     8101 cacagggtgg ggtgctcggc gtcgctgccg accgtgtcga cgtagtgcca gcagcgctcg
     8161 cacttctgcg ccgcgctcgg ggtggcgacg acgcgctcgt cctcggcgcg ggccacctcg
     8221 gacagggtgg ccgccgaggt catggtgacg aagcgcaggt cctcgcccag gctggccatc
     8281 gcggcgaact tgtcggcggt gagcgcgagc tccagttccg cctgcagcga ggcgccgacc
     8341 ttgccttcgg tgcgcagcgc ctcgatgacc ttgaggcctt ctgcacgcac ggcgcggatg
     8401 gtctcccagc gcgcgatcag cccggcttcg ccttcctgcg cgggaagggc gtggaaggtg
     8461 tgcagcatca cgctatcctg cgtaccctcg tccttgccgg ggtggatcac cgtccaggcc
     8521 tcctcggcgg tgaaggacag gatcggcgcc atcagtttca gcagcgtctg cgtgatgtgc
     8581 cacagcgcgg tttgcgcggc gcgacgcggc aggctgccgg cggcggtggt gtacaggcga
     8641 tccttcagga tgtcgaggta gaaggcgccc aggtcctcgg cgcagaacac ctgcagcgcc
     8701 tgcacgacgc gatggaactc catcttcgcg tagtcggcct cggcctgctg cgcgagctgg
     8761 cgggtgaagg cgagcgcgta gcggtcgatg tccagccact gttcgagcgg caccgcgtcc
     8821 gtggcgatgt cgaagtcggc ggtgttggcg agcaggaagc gcagggtgtt gcgcacgcgg
     8881 cgatagacct cgaccacgcg gtcgaggatt tccttcgaga tcgacagctc gccggaatag
     8941 tcggtggacg cgacccacag gcgcaggatc tcggcgccga gcttgccggt gacctcctgc
     9001 ggcaccacca cgttgccgag cgacttgctc atcttgcggc cggccccgtc caccgcgaag
     9061 ccgtgggtga gcaggctgcg gtagggggcg tggccgtcga tcgcgcagcc ggtgagcagc
     9121 gaggagtgga accagccgcg gtgctggtcg gagccttcca ggtacatgtc ggcacgcggg
     9181 ccttcggggt ggccgtcgtt gtgcgagccg cgcagcacgt gcttgtgcgt ggtgccggag
     9241 tcgaaccaga cgtcgagcgt gtcgctgatc ttctcgtact gcgcggcctc ggcaccgagc
     9301 agctcggcgg catcgagctt gaaccaggcc tcgatgccct cctgctccac gcgcagcgcg
     9361 acctcctcca tcagctcgac ggtgcgcgga tgcagctcgc cggtctcctt gtgcaggaag
     9421 aaggggatcg gtacgcccca gttgcgctgg cgcgagatgc accagtcggg gcggttggcg
     9481 atcatcgcgt gcaggcgcgc ctggccccac gcggggtaga acttggtggc ctccacggcg
     9541 cgcagcgcgc gctcgcgcag tgtggagccg tcggcggcga ccctgtccat gccgacgaac
     9601 cactgcgcgg tggcgcgata gacgagcggc gtcttgtggc gccagcagtg catgtagctg
     9661 tgggtgatct tgccgtgcga gagcagggcg ccgacctcgg cgagcttgtc gacgatcgcc
     9721 gggttggcct tccagatgtt catgccgccg aagaagggaa ggtcggacac gtactggccg
     9781 ccgcccgtca ccagggacag gatctcgtcg ttgctgcggc cgtacgcgtg ccacgagttg
     9841 aagtcgtcca cgccgtgcgc tggcgacgag tgcacgatgc cggtgccggc gtcgatgccg
     9901 acgtattcgg cgagatacac cggcgagacg cggtcgtaga aggggtggcg gaactcgatg
     9961 cgttcgagcg ccgcgccctt ggcggtggcg atgatggtgc ctgcgagctg gaagcgctgc
    10021 agcgcggatt cgacgagttc cctggcgagc acgagcagac gatcgccgac atcgaccagc
    10081 gcatagtcga actcggggtg ggcgttgagc gcctggttgg cggggatggt ccacggggtg
    10141 gtggtccaga tcaccgcggc ggcgggcttg tcgagcctgg ccaggccgaa tgcggcggcg
    10201 agtttacgca aatgagtgcc ggcgtccgcg tcggataccg ggaacgcgac atcgatcgtc
    10261 ggcgagggct tgtcggcata ttccacctcg gcctcggcca gcgccgagcc gcagtcgaag
    10321 caccagttca ccggcttcag gcccttgaac acgtagccgt tcttcgtcat ctcggcgagc
    10381 gcgcggatct cgttggcttc gttggcgaag tccatcgtgc ggtaggggtt gtcccagtcg
    10441 ccgagcacgc cgaggcggat gaagtcggcc ttctggatct cgatctgctc ggcggcgtag
    10501 gcgcggcaca gctcacgcac gcggtcggcg ggcaggttct tgccgtgcgt cacctcgacc
    10561 ttgtgctcga tcggcaggcc gtggcagtcc cagcccggaa cgtagggcgc gtcgaagccg
    10621 gccagcgtct tcgagcgcac gatgatgtcc ttgaggatct tgttcagcgc gtggccgatg
    10681 tgcaggctgc cgttggcgta gggcgggccg tcgtggagca cgaacttggg ccggccggcg
    10741 ctggccttgc ggatgcgctg gtagagcttc ttctgctgcc agtcggcgat ccagttcggc
    10801 tcgcgcctgg gcaggtcgcc acgcatcggg aagggggtgt cgggcaggtt gagcgtcttg
    10861 cggtagtcgg ccatggcggg atcggaagtc ggtgggatcg gcggggttcg tcgggccttc
    10921 aggcgggcag ggggttcgcg gccagccagt cgcgggccgt ctggctgtcg agggcgatct
    10981 gcgcgcgcag cgcgtcgagc gaggggaatt tttgctcggc gcgcaggcga tgcaggaaat
    11041 gtacgcggat gtgggcgccg tagcagtcct gcgtccagtc gaagaggtgg acctcgaggc
    11101 gggcgcggcc gttcgcggtc gcggtcgggc gcaggccgat gttggcgacg ccggccaccg
    11161 ggccggatgc gaggccttcg acgcttacca cgtagacacc gcccagcgcc gggcggcggt
    11221 gcttcatctg gatgttcgcg gtcgggaagc cgagctggcg gccgagcttg tcgccgtggc
    11281 tgacgcgacc ggcgatgctg taggggcggc cgagcaggcg cgcggcgtgg gcgaggtcgc
    11341 cctccgcgag cgcggcccgc accgccgagc tggacacccg ctcgcccgcg acggcgagcg
    11401 tgggcatgga ctcgaccccg aagccgccct cggcgccggc cgcgcgcagc atcgcgaagt
    11461 tgccctggcg gcgggcgccg aagcggaaat cgtcaccgat gaagagatgg cgcacgccga
    11521 ggccgcgcac caggatgtcg tcgatgaagg cctgcgcggg ctgagcggcg aagcgagcgt
    11581 cgaagcggca gacgtggcag cggtcgacgc cggcggcgga aagcagcagc agcttctcgc
    11641 gcagcgaggc gaggcgggca ggggcgtcgg cggggctgaa gtattcgcgc gggtgcggct
    11701 cgaaggtgag caccaccgcc ggcaggccga gcgcacgggc cttgtcggtg agcagcgaaa
    11761 gcagcgcctg gtggccgaga tgcacgccat cgaagttgcc gatggtgagc accgcagccg
    11821 tgtcggcctg ctcgggaatc ccgcggtgaa cctgcataag cgcctgaaaa agccgcgatt
    11881 atagcggcaa agcccgctat tgcgggcctt gcgggcagac ttgctcggga tgcgggcggt
    11941 gcgctcgccc tcagtcgagc cgcgccacgc gggtcggccc gctcggggcg tgctcctcga
    12001 aatagcgctt gacgccgcgc atcagcgcca tcgccatctt gtcctggtag gcctcgtcgt
    12061 tgagacggcg ctcttcctgc gggttgctga tgaaggcggt ttcgaccagc accgagggga
    12121 tgtcgggtgc gcgcagcacc gcgaagccgg cctgttccac ctcgggcttg tgcagccggt
    12181 tgatggtgcc gagctcgccg agcatggcgc gcccgagctt gaagctgtcg ttgatcgtgg
    12241 cggtctgcga caggtcgagc agggtgcggg cgatgtggcc gtcctggcgg gcgaggttga
    12301 cgccgcccac gaggtcggca tcgttctcct tctgcgccag ccagcttgcc gccgagctcg
    12361 aggcgccgcg ctcggagagc acatagaccg agctgccgtt ggcctcgggg cgaacgaagg
    12421 cgtcggcgtg gatcgacacg aagagatcgg cgcgcacccg gcgggcgcgc gccacgcgct
    12481 ggtgcagcgg cacgaagtag tcgccgtcgc gcgtgagcac cgcgcgcatg ccgggcatgg
    12541 cgtcgatctt gcgcttgagg cgttgggcga tcgacagcgt gacgtccttc tcgtagctgc
    12601 cgccacgacc gatcgcaccc ggatcctcgc caccatggcc ggcgtccagc accacggtgt
    12661 acaggcgatc gaccgccggc tcgttacgct tgccgcggcg caccggctcg cgcggctcct
    12721 cgcgcgccac cgcggtggtg ttgccgccgg cgtcgcccat cgcggcgtcc atcggcgagt
    12781 ccttcatgat cagcgccatc agcggatcgt gggcctcggt cgggtgcagg tcgagcacga
    12841 ggcggtggcc gtagttgccc accgggtcga gcgtgaaaac ttgcggattg atcgcggcct
    12901 tgagctcgat caccacccgc accacacccg gtcggttctg cccggcgcgg atcaggcgga
    12961 tgtaggggtc ggagtcgagc accttcgagg gcagggactg cagcacgctg tcgagctgca
    13021 cgccctcgag gtcgaccacc aggcgctgcg ggtcctcgac cagcatgtgg ctgtggcgca
    13081 ggcgcgagga gccctcgagg gtgatgcggg tgtattcggc cgagggccat acgcgcaccg
    13141 cgagcaggct tgccgaactc gccagcccga ccgggctcac cagcatggcg agcgaggcgc
    13201 cggcgaactt gagcaggccg cggcgatcga tgcgccccgc ggcgaaggcc tcggccgcgg
    13261 cggcaagccc cggagtctgg ccgagactca ggtcgtcccg ggcgccgggt ccgcgccccg
    13321 caaagctttc accagtccga tcacgcatgt tcgccccgct tccgtgtttg cgctgatcgc
    13381 cacatcccgc ccggattcac cggcctgcag gacgatttcg acgtcgggag agggcagatg
    13441 gggcgaggcc ttgtccggcc attccacgat gcagacgccc tcaccggcga agtattcgtc
    13501 cagccccgcg tcgagatatt cgtcgggcga ggtgaagcga taaaaatcaa agtgatataa
    13561 gtttaagcta gaaaccacgt aaggttcaat caaggtgtag gtcggactct tcaccttgcc
    13621 ctcgtggccg agcgcgcgca gcaggccgcg ggtgagggtg gtcttgccgg tgcccaggtt
    13681 gccctgcagc cagatctgca gtccgggcgc gagaacgccg gcgagcgcgc cgccaagcgc
    13741 cacggtggcc gcctcgtcgg gtaaatgcgc ctgcaggcgc gcgccgttat catcggcagt
    13801 ctggatcgac tcgatcatcg ggacctctga caaatggaaa caaaattgcc gacgccggac
    13861 ggggcggcgg tcgacgggac cgtcctcgac ggtgcggcac ttgttgcgcg gatcagacag
    13921 tgggggcgcg aactcggctt cgacgcggtc ggcgtgggcg gggtcgatct cgccgacgcc
    13981 gaacccggcc tggtcgcctg gctggaggcc ggcttccacg gggacatgga ttatatggtg
    14041 cgccacggca tgaaacgcgc gcgcgccgcc gaactcctgc ccggcagcgt gcgcgtgatc
    14101 agcgtgcgca tgggctactg gccggatgcc gcgccggcca tggacgtgct cggcgacccc
    14161 gagcgcgcct atgtgtcgcg ctacgcgctc ggccgcgact accacaagct ggtgcgcaac
    14221 cgcctgcaga agctcgccga ccgcatcagc gcggaggtgc cgcaccagta ccgcgtgttc
    14281 accgactcgg cgcccatcct cgaagtcgag cacgccagtc gcaagggcct gggctggcgc
    14341 ggcaagcaca ccctgctgct cgatcgcacc gccggttcgt ggttcttcct cggcgagatc
    14401 ctcaccgacc tgccgctgcc ggtggacgcg ccggtggcgt cgcactgcgg gcgctgcacg
    14461 gcctgcatcg acgcctgccc caccggcgcc atcgtcgcgc cctaccgggt cgacgcacgg
    14521 cgctgcatct cctacctcac catcgagctg cacggcgcga tccccgagga gctgcgcccg
    14581 ctgctcggca accgcatcta cggctgcgac gactgccagc tcgtgtgccc gtggaaccgc
    14641 ttcgcccagc ttggccggga gcccgatttc gccccccgcc agggcctcga cgacgcccgg
    14701 ctggccgagc tcttcgcgtg gaccgcggcg gagttctccg agcgcaccgc cggcagcccg
    14761 atccaccgca tcggccacgc gcgctggctg cgcaacatcg cggtcgcgct cggcaacggc
    14821 ccggcgacgc ccgccgcgcg cgcggccttg caggcgcgcg cggacgacga ggatgcggtg
    14881 gtgcgcgagc acgtcgcctg ggcgctcgcc cgcctggccg ccgccgccgg ctagaatgcg
    14941 cgcccgcaaa ccgattcagg accgcccgcc atggaagagc gcatcgtcgt gctggagacc
    15001 aagctgatgg ccgccgagga cctcctcgac gagctcaacc gcacggtctg gcgccagcag
    15061 caggaactcg acctgctgcg ccagcatctg catcagctcg cccagcaggt caagaccctg
    15121 cagccgggcg acccccttcg ccccgaagac gagatcccgc cgcactggtg agcgcggccg
    15181 gcttgcacga ggcggcggcc tgctgcgccg tcggccggcc tagccgagca gcgcgccggt
    15241 gaggctggag agggtgtcgc cgaaggcggt ggtgaggatg accaggatcg gcatgccgtg
    15301 gcagaaggcg agcacggccc ggcggatgcg cgggtgggct tcgtggagct cgaggaagta
    15361 gttgcacacc agacgcagct tgatcgccat caccagcgac acgcccaccg caaccagcac
    15421 ggcgttcgcg tcggaacggg cgatgcccgc gccgcccacg ctgagcaaca ccagcatcag
    15481 ccaggcgagg tcgatcttgc gttcggggct catgcgggtt tcctcatccg agcaggtaga
    15541 agagcgggaa gatcatcagc cagatcatgt cggtgacgtg ccagtagcag gcgaaggcct
    15601 ccagcccgct gtgctcttcg ggggtgtagg cgcccaggcc cgtacgcgcg atcacccaca
    15661 tcatgccgag caggccccag aacacatgca ccaggtgggt gaaggtcagg tagtagtagc
    15721 tgacgatgaa taccccggcg ctgccgtcga ggccgagcgc aaagttgcgc tgcagctcga
    15781 agatcttgat cgccgggtag cccagcccga acacgaagcc ggcgagcatc cagcgcaccg
    15841 tcgcgcgtcg gtcgccgcgt cgcatcgcgt gcagcgcgcg cgccacgcac cagccgctgg
    15901 tgagcagcat cagggtatag ccggtgccgg ccagcagcga gagccgctcg gggcccgcct
    15961 ggaaggcttc cgggtggtgg gcgcgggcga tgaagaagac cgcgaacagc agcgcgaact
    16021 cggtgatctc gcagaagatt cccacccaca tcgccttgct gccggggatg cgcccggcag
    16081 tcgcctgcgg cgggcggggc gccggagtcg ggacggggac ggtcggggag aggggcacgg
    16141 caagggtcac ggcggggcgg gcggcgcagg gccgcgtcga tgaggcactg agcgcaagtc
    16201 tgccagcctg gccgcttgct tcccttgatt ccgctcagga agtgcgcgcg atggcggaat
    16261 catgtttccg ttcgcgtcaa cgactcttgc gctgcatcaa catccgctga tccccgtcgt
    16321 ggtccgatgg cggccttcat ccacgcaagt cgcacgagga ggcagcccgt catggcgaaa
    16381 gtgaagttct tcggagaaga gtcgatcccg ctcgagatgc acaaggtccg catcgtccag
    16441 aagctgaacc tgccgccggt cgagcgccgg ctcgaggcga tcaccgaggc gggcaacaac
    16501 accttcctgc tgcgcaacgc cgacgtgttc atggacatgc tcaccgacag cggcgtcaat
    16561 gcgatgagcg accagcagct ggcggcgatg atgatcgccg acgacagcta cgccggcagc
    16621 gccacctaca cccggctgga gaacaagctg cacgagatct tcggcatgcc cttcttcctg
    16681 cccgcccacc agggccgcgc gtgcgagaac atcctcgccc aggtgctggt gtccccgggc
    16741 tcggtggtgc cgatgaacta ccacttcacc accaccaagg cgcacatcac gctcaatggc
    16801 ggcaaggtcg aggagctcgt cgccgacgtc ggcctcgagg tggtcagcgt gcatcccttc
    16861 aagggcaaca tggacgtcgc caagctgcgc gacgtgatcg gcctgcacgg tgccgacaag
    16921 atcgccttcg tgcgcatgga ggccggcacc aacctgatcg gcggccagcc cttctcggtg
    16981 cagaacctcg ccgacatccg cagggtgtgc gacgagttcg ggctgatgct ggtgatggac
    17041 gccagcctgc tcgccgacaa cctgcacttc atcaaggagc gcgaggaggc ctgcaagggc
    17101 ctgagcatcc gcgacatcac ccgccgcatg gccgacctgt gcgacgtgat ctacttctcc
    17161 gcgcgcaagc tcggctgcgc gcgcggcggc ggcatgtgca tccgcagcga ggagctgtac
    17221 cgccgcatgc gcggcctggt gcccctctac gagggcttcc tgacctatgg cggcatgtcg
    17281 gtgcgcgaga tggaggcgct caccgtgggc ctcgaggaga ccatggacga ggaggtcatc
    17341 aaccagggcc cgcagttcat cggcttcatg gtcgacgagc tcgtcaagcg cggcatcccg
    17401 gtgatcaccc ccgccggcgg cctgggctgc cacatcaacg cgatgcagtt cgtcgaccac
    17461 atcccgcagg cgcagtaccc ggcgggcgcg ctcgcctcgg cgctctacct cgccggcggc
    17521 atccgtggaa tggagcgcgg cacgctttcc gaacagcgcg agcctgacgg cagcgaactc
    17581 ttcgccaaca tggagctggt gcgcctggcg ctgccgcggc gggtgttcac gctgtcgcag
    17641 gtcaagtacg cggtggaccg cctcgagtgg ctgtacgcca accggcgcct gatcggcggc
    17701 ctggtgttca ccgaggagcc ggagatcctg cgcttcttct acggccgcct cgcgccggtt
    17761 tccgactggc agaacgaact cgtcgcgcag ttccgcaagg acttcggcga cagcctctga
    17821 cgccgtccgg cggcgccgtc aggcgcggag ccgccgggca ccgaccgccg gcggcacgat
    17881 gccgcggcat gccgcccgga tgcggacgcg cgcatcccga tggtgtcttg ggggtgcgat
    17941 aatgtcggtc tcgcagcctt ctggaggcct tcatgctgaa gtcccatgcg gtacttgaat
    18001 cgcgtcgtcc gtcgctgtcc gcctcggtcg cagccgcagt gctcgcgctg ggtgtcgccc
    18061 tgcccggagt ggccgtggcc gagacggtgg gccacgtcga cacggcgttc aagctgatcg
    18121 gccgcaacca ccaggtcgtg gtcgaggctt tcgacgatcc ctccgtcaag ggcgtggcct
    18181 gctacgtctc gcgcgcgcgc accggcggtc tcaagggaac cttcggcctc gccgaggaca
    18241 cgtccgacgc ctcggtcgcc tgtcgccagg tcggcgacat ctccttttcc ggtccgctgc
    18301 gcgagcagga ggaggtgttc tcgcagagcg cctcgatcct gttcaagaaa gtgcgcatcg
    18361 tgcgcatggt ggacccgaag cgcaacacgc tggtctatct cgtctacagc gacaagctga
    18421 tcgagggcag cccgcagaac agcgtcaccg ccgtgccggt cccggctgcg ctcgccatcc
    18481 cgctgcgttg agcgccccgc acgcgaggcc tgtgcgcggt gcgccgcgcc ggcttgcggc
    18541 tgcggcgaag gcgcgaaccc atgcggaggc gcgggtgcgc atgtcggcgc ctcagccgcc
    18601 gcccggctgc gacaggcggc gcgcgagtgc tccgatctcc gccgccaagc ccggcgcggc
    18661 gcggggtgct gcggcggcga aggtctcgac caggcgcgcc accacgctct catgcggcag
    18721 gctgacgcca tagtgcagcg cgacgccggc gcggaacagc gccagggtag ggaagttctc
    18781 cacgtcgaga tcaccgacga gttcggcatc gtcttccacg tccgcccagg cgaaaacgac
    18841 gtcgcgcctt gcctcggcga ggcgctcgag cgtggggcgg aactcgcggc aggtgccgca
    18901 ccactgcgcg cacagtgcga gcaccacgag gggggcgtcg gtcgcaggct gtgcggcgag
    18961 ctggtccacc ggcaggaccc gggtgggcga ggcggcggtc gtggcagaag cggtcggctg
    19021 cgcaggcgcg ccagcgggtg cgccgcaggg cgtggctccg gaaggcatgg gcgaggagcg
    19081 cccggactcc gcggtggagg gggcggaaga cgcggagggc tcggctgcgg gggggacggg
    19141 cgtggcgttc atggctcggg cttggcgccc gcccgcgcgt gaggctcagg ccgggcagac
    19201 ttcctgcatg ctgcacttgg cgatgccgaa ttcggtctcg accagcagcg gataggcggc
    19261 gccgccgaag cggcccgaac tcgcgatgtt atagccgatc acgcttccgg gcacgcgtcc
    19321 gacgagcacg cgacagccga tgcggaaacc ggcgccgatc gcgtggcgga tcgcgcgttc
    19381 ggcggtgtcc agggcggaac gggcgccggc ggccttgcgg cgaccctcga ggacggtgag
    19441 cgagcggggg tacatggtct cctcctgctg ggcgattggc ggaacgccgt gatttaacgt
    19501 tgacgttaac gttaaatccg ctccacatca agagccttgt ccaccggcgc gggcgggacg
    19561 gcgggggtca gcgcacggag gcgttggtct tgcgctgggg tacgaagctc accttgtgcg
    19621 ccaccacggt gtcgcgcatc ttcgccacgt ggggacgcag cgcgccgatg acgtgcatct
    19681 cgcagcgctt gcagtcgaac ttcagcgtca gcgtctcgtc gccattgacc aactgcatcg
    19741 ggtcggggcg gatgcccttg acctccttcg gacacagatt gaagctgtag cgcaggcagt
    19801 gcttggtgat catcagcgag acgtcgtcgc gctcctcgtt ggcctcgtat gcggcgtcga
    19861 tcagcttgac gccgtggcgg gcgtaaaagg cgcgcgcctt gtcgttcgac acgttggcga
    19921 ggtagctcag cgcgtcctgc gggtagggca ccggcggctc gaccggcgcg gcccgcagcg
    19981 ggcgggcgtg ggccgcgagg cggccggcct cgagccgctc cacggcatcg cggcgcagtg
    20041 cattgagctg tccggcgggc aggaagggcg cggcgggcag gtcgagcacg agctcgcctg
    20101 cgctgaagat cgtgttgccc agcttgccga ggtgctcgcg cagggtggcg agtgcacgct
    20161 cggcgttctg tgccggctcg aaggcggcgg caagcgtcgc ggtgacggcg acgccgtcct
    20221 cgtcggtcag ggtcagcgcg aagccgtcgt gggtggcggc gaaacgtgca tcgacacgga
    20281 tgcggcgctc ggccgacttc ttccccagcg cgcgctcgaa ttcgtggtca tggttgcgga
    20341 acagcgaggt gccggggacc aggtcggtcg gcagggggtc ggcagggaag atgcggtcga
    20401 tgccttcgcc gccgccgtcc gcctcggcgc ggttcacccg caggccggtg agctcgccct
    20461 tggggtcgta ccaggtcagg ccgtcggcgt tgtggatcgg cgccgcgcgc tcgacgtcga
    20521 agaacttgcg ccccttggtg tcgatcttct tcacgcggcc gatgggctcg ccgacgaact
    20581 tgggcgattc gaacgcctcg atgccgtggc ggcgatcgtt ggtgaagtag tccgtgtagc
    20641 cgcggttgaa ggtcttgtcg gcctgcgggg tgaagaagaa ggtggtgcgt ccgctggagg
    20701 cgcggcgata tcgggcgccg tcggtgtccg ggtgctcgag gatctcgtcg atcagcgtgc
    20761 ggtagtgcgc ggtgatgttc ttgacgtagg agagatcctt gtagcggccc tcgatcttga
    20821 acgagcttac ccccgcggcg gcgagcgcgc gcaagttggc gctctggttg ttgtccttca
    20881 tcgacagcat gtgctggttg ctggcgacgg tgtggccgtc cttgtccttg aggtcgtagg
    20941 gcagccggca ggcctgcgag cactcgccgc ggttggcgct gcgcccggtg tgcgcgtggc
    21001 tgatgtagca ctggccggag aaggccacgc acagtgcgcc gtgcacgaag tattcgagct
    21061 ggcaggtggt ggccgcggcg atcttcttca cctcgttcaa cgacagctcg cgcgccagca
    21121 cgatctgcga gaagcccacg tcctgcagga agcgcgcctt gctcgcgtcg cggatgtcgg
    21181 tctgggtgct ggcgtggagc tggatcggcg gcaggtcgag ctcgagcagg cccatgtcct
    21241 gcacgatgag cgcgtcgacg ccggcatcgt acagttgcca gatcagcttg cgcgccggct
    21301 cgatctcgtg gtcgaagagg atggtgttgg tggcgacgaa gacctcggcg tgatagcggt
    21361 gggcgtgctg cacgaggcgc gcgatgtcct ccaccgagtt gtccgcggag gagcgcgcgc
    21421 cgaaggccgg gccgccgatg tagaccgcgt ccgcgccgtg gtcgatggcc tcgatgccga
    21481 agtcggcggt cttggcgggg gcgaggagtt cgagggtgtg gcggtcgatg ctcatcggtg
    21541 gttgaagtgc gcaagcgctt gattggaaag gccgcgatga taacaaggtg tgcgccggca
    21601 ggcgcgcccg accgcaaaaa aggcgcgccg ccggtgctgg cggcggatcg tccggtgcgc
    21661 ccgtgcccgc gcctatcctt gcgcctgcgc cacttcgagc cggcgtgcca gttcggcggc
    21721 gaagttcgcg gccgcgtccg gggttgcgcc gaggaagagt tcgggctcga tggcctccag
    21781 ctccatcagc gccggccgac cctcgtggct caccaggtcg acccgggcat aggtggtcgc
    21841 cgcgggcgcc tgcgcgagca cggcctcggc cagcctgcgc tcggccgcgg ttggcgtgtg
    21901 cgcatgcacc gtgccgccgt acaggtcctg cacacgatag tcgcctgcgg cagggcgttt
    21961 gcagatggca tgggaaaaga ccccgccgaa gtagatcatc gacacctcgc ccgcctctgc
    22021 gatgctgtgc acgaagggct ggaccatgac gtcgcccgct gcgaccaggt cctccagatg
    22081 cgcgcggcag gcggggtcgt cggccctggc gcgcagggcg ccgatggcgc cgatggacac
    22141 cgcgggcttg accaccacct cgtcccagcc ctgcgcggtc agccgtgcgc cggcggcggt
    22201 gtcaccgcgt tcgatccaca gcgtgggcac ggtggcgacg ccgccggccg cgagttcgcg
    22261 caggtagccc ttgtggctgt tccacgcgat cacggctacc gggttgacga gatgggtgag
    22321 cgcgctggtg cgctcggccc aggccaggaa agcgtcgagg tggcggaagt agtcccatgg
    22381 cgtgcgtacc accacgaggg ggtacgcggc ccagtcctgt tcgctgttcc agggcggcac
    22441 gtcggcgacg atgccgcgct gcgcgagggc gtcgacgagc aggtgggttt cgggatccgg
    22501 cttggccatc gtggcgccgg tgacgagggc gatgcgcggg tggtggagca tgggcaggcc
    22561 tgttcgggag gtgtcgagcg cggaccgcat gggcccgcgt gccggcaggg agtttaccgg
    22621 gcgttcgccg tgttcgtttt ccggttgcgg aggaagggca gcaagaccag cagcccgacc
    22681 gcgtcggcga gccagtcgag cggatcgccg acgcggtagg cggtggtcga ctgcgccacc
    22741 tccatcgccg cgccgtaggc cagcagcccg gccgcgagca ggccgacgcg ttgtggccag
    22801 gcggcgacgc cgaggatcgc gaggccggcg aagagcaggg cgtgctcgac cttgtcctgc
    22861 caggagaaca gcttgatcac cggctcgatc ggcatcagcg cgagccagaa ggcgccaccg
    22921 agggcggcga tgaagagcgc gcgcagcagc gtgcgaacgg atgtgggcat ctgtggtccg
    22981 aaaaagcggt tggctgcctg tcggtggtgc ggcgcgcaca gcagcgctgg gaaggtggcg
    23041 gcggttcggt gtcgtgcgtc gatggctggc ggagcttagc ggcaaatgcg cggcgcgtgg
    23101 tgcggcgcgc aataacctgg ggagatgacc cgatgcagga tggcaaagtt cgcgaacttt
    23161 cgagctcgcg cacgcgcgca gcgctgcgcg tcgcgctgct cgccggcatg ctcgccgccg
    23221 gcagcatgca cgccccgctc gcgggcgcga ccggcatggt gtatccgagc gagacggggc
    23281 cgctgcgcgt gaccgaggtc gcgcgcggtt tggagagtcc gtgggcgctc gccttcctcc
    23341 ccgacggtcg catgctggtg accgagcgcc cggggcggat gcgcatcgtc gatacggcag
    23401 ggcgggtgtc ggaacccatc gccggcgtgc ccgaggtcca tgcgcgcggg cagggcggcc
    23461 tgctcgacgt tgcgctcgcg cccgacttcg accgtgaccg cctgatcgtg ttctcctacg
    23521 ccgagccgac cgcgcgggga gcgcgcaccg ccgtggcgcg cgcgcggctc gacgtcgacg
    23581 gcctgcgcct cgaggacgtg cgccgcatct tcgcgcagga cgaggatccc gcgggcaatc
    23641 accactgggg ctcgcgcctg gtgttcgggc gcgacggccg cctgttcgtg accctgggcg
    23701 atcggttcca ccaccgcgag cgcgcgcagg cgctcgacag ccatctcggc aaggtggtgc
    23761 gcatcgatcc cgacggcggc gtgcccgccg acaacccgct cgtggggcgt gcaggcgtgc
    23821 gcggcgagat ctggtcctgg ggccaccgca acgtgcaggg tgcggccttg cacccgcgca
    23881 ccggcgagct gtggacccac gagcacggcc cgcagggcgg cgacgagatc aaccgcaccc
    23941 tgggcgggcg caattacggc tggccggaga tcacctacgg ccgcgaatac gtcaccggtc
    24001 gcaagatcgg cgccggcagc gagcgcgaag acgtggcggc gccggtgctg caatggacac
    24061 cctcgatcgc cccctcgggc atggccttct ataccggcga tgccttcccg cagtggcagg
    24121 gcaacctctt cgtcggcgcg ctcaagttcc agctgctcgc ccgcctggtg ctcgatggcg
    24181 agcgcgtggt gcgcgaggag cgcctgctcg aaggcctggg gcggatccgc gacgtgcgcc
    24241 aggggccgga cggccgcctg tggctgctcg acgagagcgc cgggcgggtg ctgcggatcg
    24301 atccgcagtg agcggggctc gcgtgctcgt ccccggaagc ctcgcggggg cgattcacct
    24361 tagcccgcct gcttgatcgc cagcaccgcc tggttgcgca gggtgcgcag catggcgagc
    24421 tcctgctcgc tgatgcgcag ctctccggcg cgatccttgt cggcgtagat catcgccacc
    24481 gggcggccct tgatcgtgag tggaaagagc acgaaggtgc gcgccgccac cgacctgcgg
    24541 taccacgccg gcacgcgggc ggcgatcttg gggtcgtcca cgtcgctgat cagcacgtcc
    24601 acgccgcgtg cggtggccac gttgaagacg ttgtcgggct gggccgagag gctgaagcgc
    24661 agctgcttgg cgagctcgcc cacctcgggg ccgaagccga agcgcccgca catggcgttg
    24721 ctgcgcgcgt cgcgcacgca cagcagcacg cgcttgaagc ccatcgcgcg gtacatggtc
    24781 tcgaggatga tgcgcagcac gtcgttgagg cggaaatcct cgaccaagct gttgctgatg
    24841 tcctggatgc ctgcgctcag cacggcctgc gggtcggcgg cgcgctggcc ttccgcaccg
    24901 ccctcggcgg gcagcgactg ctcgagcagc gcggaggcgg gcatcagcgt ggcgtcgacc
    24961 cgtgcgccct ccgccggcgt gctgccgtcg atgaagtgac gcaggttgcg cccgaagcgc
    25021 gtctgcgcga ggcttacctt gaccacgcgg gcgaattcgc cgatctcgtc gaccgcgctg
    25081 cgcaggtgct cgcgcgcctc gcgcgcaggc accggcacgg cctcgtcgaa gcgcgcggcg
    25141 atgtcggcca gcgcacgctc gcgctccgcg ggggcgaggc gcgacaccgc atcgcaacac
    25201 gcgttggcgc aggcggccag gctctgcagg cgctcgtcgg cactgcgcgg cgcatggacc
    25261 gggccctcgg gcagggtgcg catgctgttc tggatcagct cggggaagcc ccaggcgcgc
    25321 gcgagcgcga tgccgagttc ctcgtagccg gcgccgagca cgcgctgcgc cgcgctctcc
    25381 tcgctgcagc cgtgctgctg cacgacgcgg cggatgtcct cgctctcctc cgggaagtag
    25441 aactggcaca gcagcctgcc cagattgtgg aacaccgcgc agatgtagct ctgctcaccg
    25501 tcgcgcaggc gcaggcgccg ggcgagttcg cgcgcgaaca ggcccgccag gcaggcgcgc
    25561 aggaactcct cctgcagttg gctggcgttg gacttgttct gcaggtgctc gaagagcagc
    25621 acggtgatcg cgatgttgcg caccgcatcg aagccgagca ccaccaccgc gcgcgagatc
    25681 gtgctgatgc gcccgccgcc ggcggggcgg aaatgcgcgg agttcaccac ccgcagcagc
    25741 ttgttggtga ggccgaagtc gcgcaggatc agtgccgaca gggtgcccac gctctccgat
    25801 tcgctggccg cgatgcggtt gatcgccact accgattcgg agagtgcggg aaagtcgccg
    25861 cggtgcttca tgcggcgcat caggaaatcc accgcgcccg gcctggcctc gctgccggcc
    25921 tccgggtcgg tctcggcggg ctggcgccag gcttcgagcg cgaggcggaa ctcggcggcg
    25981 tcggcaaagc gcgcgagcgg gtcgagcgcg gcggcacgca gcgcgatcgc ggccagggcc
    26041 tcgtcgaccg gggggccgtc cgcgggcagg gtgacgggtg tgctgcgcgt gcgctcgagg
    26101 accctcttca cgccgccctc gcgcgccagc ggacgaccgg cgagcagctc gatcagcagc
    26161 acgccggcgg cgaagacgtc gttgcgcggg ccgatctcgc ggcgctcgat gtactccggc
    26221 gccatgtagc ccggagtgcc cgagaggcct tcgctggccc ccgcggcctc gatgcggcgg
    26281 gcgatgccga aatccatcac gcgcgcatgg ccgccgccgt cgagcagcac gttgctgggc
    26341 ttgaggtcgc gatggatcac gccctgcgcg tgcgcctcgg cgagcgcggc gagtatctcc
    26401 agcatgcgct ccacggcctc gccggcaggc agcggcccgc gccggtcgag cagcgcggcc
    26461 aggctttcgc cgggcacgta ctcgaacacc aggtaaggat cgccgccgtc ctcgccggcc
    26521 tcgaacaccg ggacgatgcc ggggtggcgc agccggctca ccgcgcgcgc ctcggcgagc
    26581 agcgaggcgt tgtcgtggcc ggggcggggg ccgaggtgca gcgccttgag cgccacctcg
    26641 cgctcgagct gcgggtccca cgccaggtgc accacgctct gtgcgccgcg gccgagttcg
    26701 cggcggatct cgaagcggcc gatcgtgcgc gtcatcgtac cccctccgcc gccgtgccgg
    26761 cgccggatcg ggagcgtcgg agccgcgcca cggcgcgggt cgtgccgcgc tttcgtggca
    26821 tgatcgcgaa cctttcgagc acggatggat gagccaggat ggaaagtccc ttcaaaggca
    26881 agaccggtct gcgccggatc ttcaacgcgg cccgctattc gatggacgga ctgcgcgcag
    26941 cctaccggca cgaggatgcc ttccgccagg aaatatggct caccctggtc gcggttccgc
    27001 tcgcgctgtg gctgggcgag ggtgcggtcg agcgcgcgct gatgatcggc agcgtcctgc
    27061 tggtgctggt ggtggaattg ctgaactcgg cggtggaggc gacggtcgac cgggtgtcgc
    27121 tggagcggca cgagctggcc aagcgcgcga aggacgtcgg cagcgcggcg gtgctgctcg
    27181 cactgctcca cgctgccgtg gtgtggggct tgctgctgtt ctgaaagggc cgatgcgggc
    27241 cgggtgcgac gggatcaggc cgaccgcgga ccccagctgc gcaccggacc ggtgtcgatg
    27301 tgcacgaagt cggagtcggg gtagtagccg acgccgccgc cgccgagcgc gagcgcggcg
    27361 tcgcgcaggc gggcggtatc gacgcccacc aggcggatgt cgatcgcctt gccgtccatg
    27421 tgcaggctgc gcctggccac cccgccgccg cgtgtcttgc gcagcatggt gttggtggtc
    27481 ggcgagcggt agcccgagat gatctcgaag gtgtttccgc cgcacgcgag gctcaatgcg
    27541 tgaagcatgt catagaggcg cggatccatc cgtgtagact ctccggtccg aaagtcgcgc
    27601 aagagccagt cgatgcgctg cagggcgggc tggatgtaac cctggcggtt gcggaaggcg
    27661 acgctcaggc gctcgtcggt gtgagtgtgg cggaagtcga gttgcgggtc gcgctgggcg
    27721 gcctgggcgg cggccgaaag ccccagcggc agcgtggcga ggcccttgag taacaggcgg
    27781 cggtgcggca cgacctgatt ccggacgatc ttagacatgg ataataagca atccgaacaa
    27841 gtacttgagg ccggaatcct aatgcaaagc aagcccggtc tgggcaaatg ttttgtaact
    27901 gccgcggccc tcgccgcgct ggccctcatg gcgtcctgcg tgcatgcgac agcggctggc
    27961 gaggccgcgg tggtcgcggt cgagccggcc gcgagcgact ccgcgctcgc gctgcgcatc
    28021 gagcagcgcc tgcgcgagcg cgccgcgacg gccgacgacc ccatcgcgct gttctacctc
    28081 gcgcgtgcct accgctcggt gtggaccgaa cctgcgcgcg tgcacgccct gctcgcggcg
    28141 gtcgaggcgg tacgcgggca tgggctggat gcggcggatt tcgcccccgc ccgcctgcgc
    28201 gccggtgccg tccccgcggc cgatcccgag cgtgcggccg agcgcgagct cttgctcacc
    28261 gacaccctgg ccgcgctgct cttccagctc cgccatggca aggtcgaccc gcgcgcgctc
    28321 taccgcgagt ggaacttcac cccgccgccc agcccctacg agcgtgcggc cgagcttgca
    28381 cgcgtgctgc aggcgtccga tctggccgcc gcggtcgacg cgtacgcgcc ggacctgccg
    28441 ctctaccgcg cgctgcgcgc cgagctgctc gcccagcaag gccggctcgc cgtgggcgac
    28501 tggcccaagg tcgccgccgg gcccaccctc aagcccggtg caagcagctc gcgcgtggcc
    28561 tcgttgcgcg cgcgcctggc cgccgcgggc gagcgcgtgt ccgaggcgcg cgacaagtcc
    28621 cactacgacg aagctctggt cgaggcggtc aagcgcttcc aggccgcgca cggcctgcag
    28681 gccgacggcg tgctcggggc gcagaccctg gaggcgctca acgccagccc ggcgcagcgc
    28741 gtggcgcaga tccgcgccaa cctcgagcgc ctgcgctggg tggcgagcga cctgcagggc
    28801 gaccgcctgc tggtggacat cgtcggctac cacgccgacc tcgtgctcga cggccagccg
    28861 gtgtggtcct cgcgggtgat cgtcggcaag cccaagcggc gcaccccctc gctgctcgac
    28921 agcgtcaccc atctggtgct caacccgaag tgggtggtgc cacccaccat cctgcgcgag
    28981 gacgtgattc cgggcgcagc gcgcaacccg tcctatctcg ccaaccggcg cctgcgcgtg
    29041 gtcgatcgca gcgggcagac ggtggacccc gccaccatcg actggagcgg ggcgcgccag
    29101 agcggttttc cctatcgcgt cgagcagcag tccggtgccg acggctcgct cgggcggatc
    29161 aagttctcgc tctccaaccc ctacgtgatc tacctgcacg acaccaacgc gcgctccctg
    29221 ttcaagcgcg ccgagcgtgc gctcagctcg ggctgcgtgc gcgtggagaa gcccgaggag
    29281 ctggcggtgc tgctgctcgc cgacagcggg cgctggagcg cgcaggcgct gcaggcggcg
    29341 ctcgacagcg ggcgcacgcg caccgtggac gtggggcgcg acgtcaaggt gttgctgcac
    29401 tacgccaccg cggcgctcga cgaggcgggc agggtgctgc tgcgcaacga catctacggc
    29461 tacgacgcgg cgatcgtggc cgcgctcgat gcgcccgcgc cggcgcgctg aagctgtggc
    29521 ccccggcacg cgccgcggta agctcgcggc aatgccggcg taacaccgct gtcttgcggc
    29581 ccccctagtc tgcggggcca tgagaacgct cgactacacc accgaagacc tctgccgcgc
    29641 gccgcgcctg cgcatcgcgc ttgtgaccga gacctgggcg cccgaggtca atggcgtggc
    29701 catgaccctg gggcggatgg tcgacggcct catccgccgc ggccacggcg tacagctcat
    29761 ccgcccgcgc cagcgtcccg gggagaccgc ggcgcacggc gagggcctgg aagaggtgct
    29821 cgcccgcggc ctgcgcctgc cgcgctacga cggcctcaag ctcgggctgc cggcgcgggt
    29881 gcgtctcgtg cgcgagtggt cgcgccagcg cccggacctg gtgcacgtgg cgaccgaggg
    29941 gccgctcggg tggaccgcgg tcaccgcggc caacaagctg cgcatcccgg tcagctccga
    30001 cttccacacc aatttcgacc actacagcgg ccactatggc atgggctggc tgcgccagcc
    30061 ggtggcggcc tacctgcgcc gcttccacaa ccgcagcgcg gtgaccttcg tgccaaccgc
    30121 ggcgctcgcg gcgcaactct cggcgcaggg ttaccgcagc gtggaggtga tctcgcgcgg
    30181 ggtcgacacc gcgctgtatt cgccggcgcg ccgcgacgag gccttgcgcc gtgcctgggg
    30241 cctacccccg ggcgggctgg cggtgatcag cgtcggccgc ctggcgccgg agaagaacct
    30301 cggcctcgcg atgcgcgcct tcgcagcgat ccgccgcctg cgcccggacg cgcgcatggt
    30361 cctggtcggc gacggccccc agcgtgcggc gctggcgcgc gcccaccccg acgccgtctt
    30421 cgtcggcatg cgtcacggcg aggacctcgc cgcgcactat gcgtcggccg atctgttcct
    30481 gttccccagt ctcaccgaga ccttcggcaa cgtcaccctc gaggcgatgg cgagcggcgt
    30541 gtgcccggtg gcctacgact acgccgccgc cgccgaggtg atccgcgacc tcggcaacgg
    30601 tgccagcgtg gcctgtggcg acgaagaggg cttcatcgcg cgtgccgtac agatggccgg
    30661 ggccgatgcg ctgcgcgcgg agctcgcgcg cgccgcgcgc cgcagcgccg aggcgatcga
    30721 ctgggagcgg gtgaacgacc gcttcgccgc ggccctgctg cgcgtgtggc gggcgggcag
    30781 cggccggccc ctcgatttgt ccgaaccccg cccggaggag acctgaacca tgagcagcgt
    30841 gcgcgcgatc ttcctctccg atgtccatct cggcacccgt gcctgccagg ccgagcgcct
    30901 gctcgacttc ctgcgcgagc acgagtcgga ctaccttttc ctgctcggcg acatcgtcga
    30961 cttctgggcg atgaagcgca gcgtgcagtg ggcgcccagc cacaacaccg tggtgcagaa
    31021 ggtgctgcgc cgcgcccgcc acggctgcca ggtgatcttc attccgggca accacgacga
    31081 ggccttgcgc gagcatgccg gcgtcgcctt cggcgacatc cgcgtcgagt cggaatggat
    31141 ccaccacacc gccgacggcc ggcgctactg gctggtgcac ggcgacgagt acgaccaggt
    31201 cacccgccac caccgctggg tggcgctgct gggtgacgtc gcctacaacg cgctggtgcg
    31261 catcaacctg ctgctgtcgc gtgcgcgtcg cctgctcggc gtgccgggat actggtcgct
    31321 cgcgggctac gccaagcaga aggtgaagaa ggcggtgagc ttcatcttcg acttcgagga
    31381 tgcggtcgcc cacgccgcgc agcagcgcgg ggtggacggg gtgatctgcg gccacatcca
    31441 ctgggcctcg gatcgccgca tcggcgacat ccgctacctc aattgcggcg actgggtgga
    31501 tagctgcagc gccgtcgtcg agcaccacga cggccgcatc gaggtgctgc agtggggcgc
    31561 gctgggcgcg cacgcgctcg ctccgctgcc ggcgctgcct gcaccgaggc ccgcgtccgt
    31621 tcgaccgcaa tcggcgccgg ttccggcggg tgccgagcgc atctacctga tgccggacat
    31681 ctgaacccgc gaggcccctc ggccgccgca ttcgcgcctt gcggcgctcc tgcatcggca
    31741 gggaccgtgg cggttttcgg taggagcgcc gcaaggcgcg aatatggccg ccgcgatgta
    31801 ccgcttcccg ctgcctgggg ccagcggcca gtcggatcaa tcctcgcgct cgtcgcgcag
    31861 cagatcccag gcgatgaaca ttccgagcgc ggcgttgccg agcgcgaaca gcgccaccac
    31921 cacggtcgcc agcggaacca gcggccagct gcgcaggagc caggcaagga tggccgagag
    31981 caggttgagc acgatcatca gccagaagcg gcggtcttgc gggcggtaga
//
LOCUS       DAOQBS010000022        31247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_843_length_31247_cov_16.449635, whole genome shotgun sequence.
ACCESSION   DAOQBS010000022 DAOQBS010000000
VERSION     DAOQBS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 31247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31247
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_843_length_31247_cov_16.449635"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PKN25_04435"
     CDS             <1..647
                     /locus_tag="PKN25_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNV90178.1"
                     /translation="CGANEADFHFTGVNWGRDLPEPDQVADLRNVVEGDPSPDGRGVL
                     AIQRGIEVGHVFYLGTKYSKAMNATFLDEDGKPKHFEMGCYGIGVTRILGAAIEQNHD
                     ARGIVWPRAIAPFEVVVCPVGWGKSQAVRDEAQKLYDALVATGVDAILDDRDERPGVM
                     FADWELIGVPHRVTIGDRGLKEGVIEYQGRRDAEAAKLPAGEVLGHVLERLNQG"
     gene            665..1330
                     /locus_tag="PKN25_04440"
     CDS             665..1330
                     /locus_tag="PKN25_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNV90179.1"
                     /translation="MRRLPAPLRQLAAACALALAAGSAQAGAQQYEPMAASVRAALHA
                     AVSDAVAPTLPIPDAGERAQWLAEMSRRLARRIPDEAYRRELLTSIHYEATRAGLDPQ
                     MVLGLIQVESAFRKYAISSAGARGYMQVMPFWIKVIGREDDNLFHMRTNLRYGCTILR
                     HYLDIEKGNLFRALGRYNGSLGKPDYPNLVRAAWERHWTWTPQQLASAGASQPQSGAT
                     TAR"
     gene            complement(1450..2019)
                     /locus_tag="PKN25_04445"
     CDS             complement(1450..2019)
                     /locus_tag="PKN25_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNV90180.1"
                     /translation="MFNKPNLFHGTGEVSAQRNAQRTAGTNVAAVPVTPPAETGGSAA
                     AREPQAVERADAAAAAGAVGESRLIVGPEVKLKGAEILDCDTLVVEGRVEATMDSRVL
                     RIAEKGAFKGTVGIDIAEIHGHFEGELTARKQLLIHAGGRVSGKIRYGKILIEEGGEL
                     SGDVQSLGAAGKELRAIDDKPRALAAVAG"
     gene            2364..3824
                     /locus_tag="PKN25_04450"
     CDS             2364..3824
                     /locus_tag="PKN25_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNV90181.1"
                     /translation="MHHLSFRSILLASFLLITASLAAAVAGGWRGMETVVRAIQEDKQ
                     RALALSAAARQLRERSVNLERSARQYEVLGEPALARRFEATLAEALPALALLESAGPA
                     LASAGAAWRACADRIAAWLASLAIPGPTDGVREQPMPATAAGRGDGLATADDFRHLGA
                     LADRLAAALDHHLGERDLALMDTLERERRAVALQILAALLLAGLLAALAGGWLLRPLG
                     RMEQAIGELGENRLTRPIDIDGPADLRRLGERLDWLRLRLAELEADRGRVLRHVSHEL
                     KTPLASLREGVALLEDGVLGPLEPEQREVTAILAHSARTLQARIEQLLRLNASQFDAR
                     SLHLQPTALLPLLRKIGEEQRLPARARRVTIGLAGDAPIVQADAGKLHSVFSNLLANA
                     IAFSPTGGHIRIELRRAGENVVIDCRDEGPGIAPDELERIFEPFYQGRRSPPSSAGGS
                     GIGLAIVREFIGAHRGRVRALPSELGAHFRVELPHA"
     gene            3895..4362
                     /locus_tag="PKN25_04455"
     CDS             3895..4362
                     /locus_tag="PKN25_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90182.1"
                     /translation="MLPATLALLAGCADLPARQAAEDDTSAAALAHEAAQQRLHTAVQ
                     AAQPGQERLATARRALESLLADDRAQARALHPYARALLEQLRVRQQVAALAEHLRRQL
                     DERARASDAQALELDALRRQNAALQRQLEALAEIERELTSPGLPVRPRTGLPE"
     gene            4377..5786
                     /locus_tag="PKN25_04460"
     CDS             4377..5786
                     /locus_tag="PKN25_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV90183.1"
                     /translation="MHATAAAAPARLLVVDDDADLLRLLAMRLQATGYDVATADSVAA
                     ARSRLGVERFDLVVSDVRLPDGDGLALFEDIRRQHPALPVILLTAHGTIPDAVEATAL
                     GVAGYLTKPFDSQALLQGIRQALQRAGPTHARSGLRADAPGSAGDTAWRAAILSRSSR
                     MQAVLDEARLVAAADASVLIRGDSGTGKELLARAIHLASPRAAAPFVAINCGAIPEPL
                     LESELFGHVRGAFTGATSAHQGLVQAAHGGTLFLDEIGDMPPALQVKLLRVLQERAVR
                     PVGATRAQAVDLRIVSATHRDLDAALAAGQFRADLYYRIDVVSLQLPTLAERREDIPL
                     LAGHFLRSLAHKYGRRLTGFAPEALEALATAPWPGNVRQLHNVVEQACALATTALIPR
                     ALVERALRVQPVEALGYAEAKRRFERNYLIQLLKLTAGNVSDAARLAERNRTEFYRLL
                     QRHDLTPELFRGAGPDVEQ"
     gene            5810..5995
                     /locus_tag="PKN25_04465"
     CDS             5810..5995
                     /locus_tag="PKN25_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90184.1"
                     /translation="MIKRQTIAAMLTTTAIAFSIATPAQAARLLEAGPFDTVSACSGD
                     KPATATPGERKDKPAQT"
     gene            complement(6137..7582)
                     /gene="tldD"
                     /locus_tag="PKN25_04470"
     CDS             complement(6137..7582)
                     /gene="tldD"
                     /locus_tag="PKN25_04470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HNV90185.1"
                     /translation="MSKSQNPLKVADRYLLSPYGLGEAELEKTFRKLMRHDIDFADLY
                     FQYQRSEAWSLEEGIVKSGSFDIEQGVGVRAISGEKTAFAYSDDISLDALVGAATATR
                     AIAAAGERRRVGIVPHKLRTRLYRADDPLDSLDDTAKVELLERLEAFARAEDARVTQV
                     MAHIAGSWEVVMVVRSDGHQAADVRPLVRVSITVIMEEDGRREQGSAGGGGRYDYGWF
                     CDDRLHDYARQAVHQARVNLGADPAPAGTMPVVLGSGWPGILLHEAIGHGLEGDFNRK
                     GSSAFSGRIGQQVAARGVTVVDDGTLPDRRGSLSIDDEGNPTERTVLIEDGILTGYMQ
                     DMMNARLMGMPVTGNGRRESFAHLPLPRMTNTYMLNGDKDPEEIIKSVKKGLYAVNFG
                     GGQVDITSGKFVFSTAEAYLIENGKVTRPVKGATLIGNGPDALTKIRMIGNDMRLDPG
                     VGTCGKDGQSVPVGVGQPTLRIDGLTVGGTA"
     gene            complement(7703..8527)
                     /locus_tag="PKN25_04475"
     CDS             complement(7703..8527)
                     /locus_tag="PKN25_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNV90186.1"
                     /translation="MTETSLSAPVRIAAIQTVSGPDVAANLETASHLIAQAAAAGARV
                     IALPEYFPLISKDETAKVRIREPEGAGPLQDFLAGEARRHGVWLIGGTVPLVAEADDK
                     VRNSTLVFDDQGRRVARYDKIHLFGFQRGEERYDEAATIEPGGTVACFDSPAGRIGLS
                     VCYDLRFPELFRAMGEVDLIVLPAAFTWTTGRAHWEVLLRARAIENQCYVMAPAQGGC
                     HESGRVTWGHTMIVDPWGEILACRDEGPGVVVADLDPARLAAVRGSLPALRHRCLA"
     gene            complement(8622..12647)
                     /locus_tag="PKN25_04480"
     CDS             complement(8622..12647)
                     /locus_tag="PKN25_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HNV90187.1"
                     /translation="MRRQRCNRAPTQRPASIRLSPSAPHAPAESVPPSVDGLPAARGG
                     WRRAAGRLSDALLLVWCVLAVGVLVLRHLVLPAVGELRVPIAELLGERLGVAVAIERI
                     EGGWAGWRPRLRLAGVSLSDAQGRPALTLPEIDATLAWSSLAMLEPHFHRLEIRAPDL
                     LVRRDPEGALHVAGIALAHGDEGGDGGLGWLLAQRQILIHGARLAWADEARGAPVLEL
                     EEVEFRLDRRTRPARFALEARLPAALGERASLRGELRRIDPLRPQRFAGRLYVELGRT
                     ALGNWRPWVDLPFGLEGEGALQAWLDADGRGGHALTADLALDGVRAVLGEDLPVLELA
                     RLDGRVELARGEDGFRFAGRGLGFETAQGLVLPPTDVELGLDPALDGKARDRGTGEEQ
                     AGRGPAAAGGRLVADRLELATLVALAAHLPLDAGLRSRLAALAPRGRVEGLRLAWKGE
                     PAAPEHWALTASFAGLGLAAQDGLPGVTGLSGSVDGDRQRGRFRLAAADMRIDLPAVF
                     DPGPLDFSRLQAQGGWQRAEGRLAIVLDEAAFENADAAGTAAGRYWPATTGAGEIDLQ
                     ARLTRAESTAVWRYVPRAVNDDTRDWLRRGIRSAKVPEARLELRGALDDFPFRAGQGR
                     FLVSVQVADAALDYAPGWPGIEGIDGEVRFEGPGLRILVPRGRIFGVDLVDVVAEVPD
                     LDQLPSEIMTITGRARGPTAEFLRFVSASPVSRRIDGFTDGMVAEGRGELELELVMPL
                     RATIDSRVQGEYRFADNRIALLEALPPLEAARGRLRFTADTLSIPEAQARLFGNPLRL
                     SAQTGEDGTVRFRAAGRADAAALAAAWEVPLLDELAGTAEWSADIRVGRRGTRVEIAS
                     DLAGFACSLPPPFGKSAAEGWPARLAFERPAGSQDARITLAIDDRLRAELLRSAGGVV
                     RGGVALGRASADAPPGSEAGVRVAAVLDRLDLDAWRAVLERAAPLPAAGAEEAAVAGD
                     GAPAAVAEFALAADEVGVFGQTFKAVDLRARADPGGWKARLESDRADGEFDWRAAGKG
                     TLAARFRHLRLVREAGGAQAGADGDSAGDEAVDDPPQRLPALDVVAERFDINELELGR
                     LEVLARNRGNLWQLDRFALMNADGRLAGKGQWRAGARQRTELDFALETASMGGLIRRL
                     GHPDVVRGGAASLAGQLAWRGAPTRIDYPSLSGRLRLETGAGQFNRLEPGVGRLLGIL
                     SLQSLPRRITLDFRDVFSEGFAFDRISGSIELARGVLRSEDLEIRGPAARVAMSGSAD
                     VVSETQDLRVLVQPTLSESVAIGAAAGLVNPVAGVVTYLAQKVLSDPIEKLFAFEYTV
                     TGAWSDPQVAKRGRAAAPESR"
     gene            12766..15474
                     /gene="glnE"
                     /locus_tag="PKN25_04485"
     CDS             12766..15474
                     /gene="glnE"
                     /locus_tag="PKN25_04485"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HNV90188.1"
                     /translation="MSAPNSVSAPALPPAVAQARRLSRYLARMLDSQPWLAEALAASI
                     ERPLAEADLQRFVAAREVLHGGGDGALRPALRQLRMWTLCHLIVRDLALGAPLAEVTE
                     TMTLLAEVALRHAHDVLRAGLVERHGQPLSPTGWEQELLVVGMGKLGGRELNVSSDID
                     LIFIYPEDGDTGGRKAISNYEFFEKLGKQLIQALAEITEHGQVFRVDMRLRPNGDSGP
                     LVCCFDMLENYFVTQGREWERYAWIKARVMLGERWPELEQLARPFVFRKYLDFGAINA
                     MRELHAQIRREVARRERANNVKLGPGGIREIEFIAQVFQLIRGGRDTALQVRPTLAVL
                     ARLPERGILSEEAVTELSNAYVFLRRLEHRLQYLDDAQTHDLPEKDEDRALIAEAMGF
                     ADYAALLAVLDAHREVVSRHFDSVFGDPSEEDHSLDATWANARDTEAATGALGELGYR
                     HPRMAAERLAAMHASPRYRQLPNNIKSRFDALMPRVLEAAASTPGADETLARMLDLIE
                     AIGRRGAYLALLQQYPQALRRVADLMSASRWGAQFLTRHPILLDEMLDARSLDNAPDW
                     KAFRASLAEELEALEPDMERQMDVMRERHHAQVFRLLTQDLAGLLTVEKLADHLSELA
                     DIVLDLTLPLCWRRIKIRHREAPKFAVISYGKLGGKELGYASDLDIVFLHDDEAGEAP
                     EVYTRLAQRVSTWLSSQTAAGQLFETDLRLRPNGESGLLVSSLESFRSYQLESAWVWE
                     HQALTRARFSAGDRTIGEAFERIRCEVLCMRRDLPSLRAEVLAMRHKMRDAHGSRSEE
                     VFDLKHDEGGLIDVEFLIQYLVLGHSHDHPRLTGNLGNIALLGIAGELGLVPADLAAA
                     CADSYRELRRLQHRQRLNERPSRVDPAEAAAARKPVRALWRAVFGD"
     gene            15686..17215
                     /locus_tag="PKN25_04490"
     CDS             15686..17215
                     /locus_tag="PKN25_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNV90189.1"
                     /translation="MSHEIELKLALPRRALPALRRHPLVAAAEKCGNALTLDNTYYDT
                     PKLQLKARKVAVRTRRQGRQMLQTVKCAAVSSGGLSQRPEWETAWTGGFDFTPVDDPA
                     TARLLERHRAELVPVFTTRFRRETRRLVPQAGVSILLMIDTGAVHVRTPEGVEREAEI
                     CELELELESGRAQDLLDLACTLAQDLPLMPADLSKAERGYRLFLDTPAGALRSEISTL
                     EPGQNVVEAFQGLALSCVRQWQGNAATALAQGDPDAIDPDNIHQLRVAHRRLRALLKI
                     FAPALPETFAGTWNARLRDNANRFGDARDLDVFHAELLEPVTPEGLADAASMAALLET
                     VRTARASARHHAGVSLDLATQGRLLLEFTAALHRLRADSLAEAADLRSFARLRLDRLR
                     KRARRGARAAASLEPTRLHALRIDFKMLRYGVEFFAPLFGTRSITRYLDGVVRAQTTL
                     GFLQDVDTAHQRLADWSRTQPALAAAAAFVLGWHAPRYARLRRRVLRECEPLLWGGKP
                     W"
     gene            17311..17763
                     /locus_tag="PKN25_04495"
     CDS             17311..17763
                     /locus_tag="PKN25_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNV90190.1"
                     /translation="MDLLLWRHAEAVDGSPDHTRELTPRGLKQARRMARWLEDNRPKN
                     LKVLVSPTLRTRQTATAFTDDFEIVGGLGPDGNVADILAATGWPEGNGAALVVGHQPA
                     LGRLCALLLSGSEADWTIKKGALWWFTNRVREDETQTVLRAVIPSELV"
     gene            17963..18529
                     /locus_tag="PKN25_04500"
     CDS             17963..18529
                     /locus_tag="PKN25_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNV90191.1"
                     /translation="MLERFLPTREAITQSRMLRWLGPRLHDPLLWHVNRRSVARGVAM
                     GVFFGLMIPIAQIPAAAIASLLLRGNLLIAAASTLVSNPLTYGPLYYFAYQLGAGVIG
                     RRTPAALTADDVEAPVRAIDSLAQAWAWIGGIGQPLLVGMVIMAVTGALVAYWGTQLV
                     WRLRVAARRRRQRRERSARASAQAIGSA"
     gene            18582..19538
                     /locus_tag="PKN25_04505"
     CDS             18582..19538
                     /locus_tag="PKN25_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90192.1"
                     /translation="MEECIPDGTVAIIDGVRRIYYDGYWLKAYEPPADTLDAKKRLIQ
                     ALTRRLFNHVEHGINIPGKRLDDARRAYETEEDPARKRVKGAMYAGALFNRATDIFTK
                     LVELQELGIEIATDNPLMRECGGCLQEALKLGRLVLHRSGDEGIDELWGEPFRAFSIP
                     VEAFYESRYIKIAQALREVDRIAAVMALTFAATPLFRGVEPLVFEFARIAKIKCETLR
                     TDPDIFDVWAEFVTASERLAALTPALGPGADECTRQLAADGLHLILQGRNLVTDIIRA
                     RVTMPKSAREFIERCETFAARAQAASHPPLAAANVGLGETRA"
     gene            complement(19616..20713)
                     /gene="mnmA"
                     /locus_tag="PKN25_04510"
     CDS             complement(19616..20713)
                     /gene="mnmA"
                     /locus_tag="PKN25_04510"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNV90193.1"
                     /translation="MKVVVGMSGGVDSAVTALLLKRQGYQVTGLFMKNWEDDDDSEYC
                     STRQDLVDVASVCDVIGIDLEVVNFSAEYKDRVFADFLHEYEAGRTPNPDVLCNSEIK
                     FRCFLDHAMQLGAERIATGHYAQVREWANDGRSEYQLLKAEDGTKDQSYFLYRLNQEQ
                     LAKTMFPLGALYKREVRRIAAEAGLHVAEKKDSTGICFIGERPFREFLMRYLPTRPGE
                     IRALDDGRVLGEHQGLMYHTIGQRKGLHIGGLKGHQDEAGEHEAWYVAQKDVKANVLY
                     VVQGHAHPALLRDHLVAGELNWIAGRDPHSHWVYTAKPRYRTPDQPCEIDRIADGRAE
                     IAFAQPQWAVTPGQSVVVYESKVCLGGGIIL"
     gene            complement(20742..21425)
                     /locus_tag="PKN25_04515"
     CDS             complement(20742..21425)
                     /locus_tag="PKN25_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90194.1"
                     /translation="MAPALRLLLPALLALPATTWAMAGAQGPAGGARVNICYNYGCAS
                     EGSVRVRESTLRRIGERLAAARSAGEERARLAEAVGGLYRVAATQTAIAADRAGNLLD
                     EGADGRMDCIDHSTSTTRLLQLLEARGALRFHRVVEPARRTRLILQHFSAVIEALSVE
                     ERFERLPPGQALAGCNCTEDGLVIGEMDGDDRPGQRYVVDSWFVDNGEPAVVLPLAEW
                     LNGGGPNVQ"
     gene            complement(21434..21886)
                     /locus_tag="PKN25_04520"
     CDS             complement(21434..21886)
                     /locus_tag="PKN25_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNV90195.1"
                     /translation="MKDRPWKPNVTVAAIIERDGRFLLVEEETPEGLRFNQPAGHLEE
                     GESLLQATVREVLEETAHHFEPEYLVGIYQWTRPQGDITYLRFAYGGRLCGEESGRRL
                     DEGIVRAVWLTLDEVRACADRHRSPLILQCIEDWIAGQRHPLALVHHR"
     gene            22126..23046
                     /locus_tag="PKN25_04525"
     CDS             22126..23046
                     /locus_tag="PKN25_04525"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNV90196.1"
                     /translation="MSMADRDGFIWQDGKLVPWREATTHVLTHSLHYGLAVFEGVRAY
                     NTVSGTAIFRLAEHTQRLINSGKIYMMDIPYSKDELMEAQKEVVRANKLESCYLRPIA
                     FYGSEKMGISTLGARVHVAIAAWPWGAYLGEEGLQKGIRVKTSSYTRHHVNSTMPRAK
                     LSATYPNSILANLEVTRMGYDEALLLDNQGFVAEGAGENLFIVKDGRIYEPEIASALT
                     GITRDSIHVIARELGYEVGTKRLTRDDIYLADEAFFTGTAAEVTPIRELDDRQIGEGR
                     RGPVTEKIQTRFFDVVNGRAPEYAHWLAHV"
     gene            23071..23271
                     /locus_tag="PKN25_04530"
     CDS             23071..23271
                     /locus_tag="PKN25_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HNV90197.1"
                     /translation="MVENTDKTQAVVTTAKDLPLHCPPPGAPLWARHPRVFLDILKTG
                     EAVCPYCSAHYVFNGERPKGHH"
     gene            23371..23799
                     /locus_tag="PKN25_04535"
     CDS             23371..23799
                     /locus_tag="PKN25_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNV90198.1"
                     /translation="MSRPVFTTALEAEAAFYRALSEGDFEALMAVWSEEEEVVCVQPG
                     GPRIVGLAAVREIWRQILADGARIRIDISHAVTSSTAMMAMHCVLERLATDGPTPRTA
                     TIAATNVFVRGADGWRMVLHHASPVPEISGPEDSTPRVLH"
     gene            23934..25331
                     /locus_tag="PKN25_04540"
     CDS             23934..25331
                     /locus_tag="PKN25_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNV90199.1"
                     /translation="MRHPLPAAELFKAYDIRGIVDTVLTPTAVRAIGHAIGSEAAVRG
                     QRAIAVGRDGRLSGPALAGALTAGIRAAGIDVIDIGCVPTPLTYFAAFELGCASCVSV
                     TGSHNPPAYNGLKIVLDGRTLHGEAIQDLRRRIAEDDLVEGAGALRGADVRAAYLDRI
                     VGDIELARPLKIVLDCGNGVAGAVAPELFRRLGCELVELFCEVDGRFPNHHPDPSRPE
                     NLQDVIRALRETDAELGLAFDGDGDRLGVVCKDGEIVYPDRQLMLFAADVLARVPGGE
                     ILYDVKCTRNLAPWIRAHGGRPTMWKTGHALLKAKLQETGAALAGEMSGHMFFKERWY
                     GFDDGLYAGARLLEILSARADANAALKALPDATSTPELNLKMQEGEPFELVRRLREAA
                     LATPSCFAEAREVITLDGLRVEFAHGFGLARPSNTTPMVVLRFEADDAAALASIEAAF
                     RRALDAVWPGLALPF"
     gene            25523..26401
                     /locus_tag="PKN25_04545"
     CDS             25523..26401
                     /locus_tag="PKN25_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV90200.1"
                     /translation="MTAREQREARARDSRQAQKGIDIPSCPGILAQLMDELRQPQVSG
                     KRIAALIGQDVGLAAVVVRSANSPYFGSGRRIASIDDAIRMLGFGMLANLVQEALLRS
                     NIADPGAALERFWDSSRCTAQASAMLARRTGIARPETAYTFGLFHDCGIPLLVRRFPE
                     YKRILATANQATGRWFTDVEDEALDTNHATIGYLLARSWGLTDAVCTGILCHHDYSVF
                     SPDSGLEVEAQTLIALNLVAEYVAGTHLRTRRDAEWDKGREAVARFLGYAPTELDDLA
                     DDLLYALDRQGEDLPA"
     gene            complement(26439..28673)
                     /locus_tag="PKN25_04550"
     CDS             complement(26439..28673)
                     /locus_tag="PKN25_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNV90201.1"
                     /translation="MSTLLSNLNPEQLQAVSLPAQHALILAGAGSGKTRVLTTRIAWL
                     IQSGQVDPAGILAVTFTNKAAKEMLARLGAMLPVSTRGMWIGTFHGLANRLLRAHHRD
                     AGLPQLFQILDSADQLAAIKRLLKTLNVDDEKFPPRELQHFINGQKEAGNRPHVVEAW
                     DDYTRLRVQLYQEYEAQCQRESVVDFAELLLRSHELLERNEPLRRHYQRRFRHILVDE
                     FQDTNRLQYRWLKLFADEGREGGAAMFCVGDDDQSIYRFRGAEVGNMRDFEREFRVNN
                     VIRLEQNYRSHANILDAANAIILHNSNRLGKNLWTDQGAGEPIRVFEAYADADEARWI
                     VEEIQALVRDGALRSEIALLYRSNAQSRVLEHQLFAAGIPYRVYGGLRFFERQEIKHA
                     LAYLRLIANPDDDTSFARVVNFPTRGIGARSLEVLADAARTYNTSLYATVPHLSGKPG
                     SALAVFVRLVEELRRDTAGLPLPELVDHVLDLSGLRAHYKADKEGRERLENLDELINA
                     AANFLAEAQAQAGAGEAQPHALLADFLAHASLEAGDHQADQGADAVQLMTVHAAKGLE
                     FDVVFLSGLEEGLFPHENSILEQDGLEEERRLMYVAVTRARERLYLSFAQSRMLHGQT
                     RYNLRSRFLEEIPAGLTKWLTPPQPRSAAGGAMGGMGGGYFKPSAKSAVGSTPQVQRA
                     PRPAFATLPGGLRIGQAVAHAKFGQGVIVAAEGSGSDAKVQINFGPAGVKWLLLGVAK
                     LEPV"
     gene            29016..29345
                     /locus_tag="PKN25_04555"
     CDS             29016..29345
                     /locus_tag="PKN25_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90202.1"
                     /translation="MKTPKLLPWYARKAGVSIERAETLWRKAVREATAETGWVGNAEY
                     WGAAMDHFVRLLEEERSTLCAPRVQSYVRTQNRMWRLPLIAMEDLFSAFHASWQRQHG
                     ADHRRAA"
     gene            29611..>31247
                     /gene="leuS"
                     /locus_tag="PKN25_04560"
     CDS             29611..>31247
                     /gene="leuS"
                     /locus_tag="PKN25_04560"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNV90203.1"
                     /translation="MQEKYQPAALETAAQQHWDSTDAFRVTEDASRPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLARFHRMKGYNVLQPMGWDAFGMPAENAAIQNNVPPAKWTY
                     ANIDYMKAQLKRLGFAIDWSRELATCTPEYYRWEQWLFTRLYEKGLIYKRLGTVNWDP
                     VDETVLANEQVIDGRGWRSGALVEKREIPMYYMKITAYADELLSALDDLPDWPEQVKL
                     MQKNWIGRSEGVEISFPFDVSSIGHEGALKVFTTRADTLMGATYVAVAAEHPLATQAA
                     ASNPALAEFIEECKRGGVAEADIATMEKKGMPTGLKVRHPLSDEPLEVWVANYVLMGY
                     GEGAVMAVPAHDERDFAFATKYELPIRMVVGADAYADTVAPWQDAYAEYGRLVNSGKY
                     DGLDFQGAVDAISADLEARGLGAKRVQFRLRDWGISRQRYWGCPIPMIHCADCGDVPV
                     PDDQLPVVLPENVEITGRGSPLAKMASFYECSCPKCGKPAKRETDTMDTFVESSWYFL
                     RYACADSTTAMVDARVNYWAPVDQYIGGIEHAILHLL"
BASE COUNT         4642 a        11761 c        10512 g         4332 t
ORIGIN      
        1 tctgcggcgc caacgaggcc gacttccact tcaccggcgt gaactggggc cgcgacctgc
       61 ccgagcccga ccaggtcgcc gacctgcgca acgtcgtcga gggcgacccc agcccggacg
      121 gccgcggcgt gctcgccatc cagcgcggca tcgaggtcgg tcacgtgttc tacctcggca
      181 ccaagtactc caaggcgatg aacgccacct tcctcgacga ggacggcaag cccaagcact
      241 tcgaaatggg ctgctacggc atcggcgtga cccgcatcct gggcgccgcg atcgagcaga
      301 accacgacgc ccgcggcatc gtctggccgc gcgcgatcgc acccttcgag gtggtggtct
      361 gcccggtcgg ctggggcaag tcgcaggcgg tgcgcgacga ggcgcagaag ctctacgacg
      421 cgctcgtcgc caccggcgtc gacgccatcc tcgacgaccg cgacgagcgc ccgggcgtga
      481 tgttcgccga ctgggaactg atcggcgtgc cgcaccgcgt caccatcggc gaccgcgggc
      541 tcaaggaagg cgtgatcgaa taccagggcc ggcgcgatgc cgaagccgcc aagctgcccg
      601 caggcgaggt cctcggccac gtgctcgagc gcctgaacca gggctgaccc ccggggaacc
      661 cgccatgcgc cgcctgcccg ccccgctccg ccagctcgcc gccgcgtgtg cgctcgcgct
      721 cgcggccggt agcgcgcagg ccggcgcgca gcagtacgag cccatggcgg cgagcgtgcg
      781 cgccgcgctg cacgccgcgg tcagcgacgc cgtggcaccg acgctgccga tccccgacgc
      841 cggtgaacgt gcacagtggc tcgccgagat gtcgcgccgg ctggccaggc gcatcccgga
      901 cgaggcctat cgcagggagc tcctcaccag catccactac gaggccaccc gcgccggact
      961 cgacccgcag atggtgctcg ggctgatcca ggtcgagagc gccttccgca agtacgcgat
     1021 ctcctcggcg ggggcgcgcg gctacatgca ggtgatgccg ttctggatca aggtcatcgg
     1081 ccgcgaggac gacaacctct tccacatgcg caccaacctg cgctacggct gcaccatcct
     1141 gcgccactac ctcgacatcg agaaaggcaa cctcttccgt gcgctgggac gctacaacgg
     1201 cagcctgggc aagccggact accccaacct ggtccgcgcc gcctgggaaa ggcactggac
     1261 ctggacgccg cagcagcttg cctccgcggg cgcttcccag ccgcaaagcg gcgccacgac
     1321 ggcgcgctga ccccaccagg gcatcgcgct cacgaggggc actaccgccc ctccacgcag
     1381 caccgcacgg ctcgcgccat gcgcggacac gtccgctgcc atgccgggac ctgccccctg
     1441 ccgacccgct cagcccgcca ccgcggcgag cgccctcggc ttgtcgtcga tcgcacgcag
     1501 ctccttgccc gcggcgccca gcgactgcac gtcgccagac agctcgcccc cctcctcgat
     1561 gaggatcttg ccgtagcgga tcttgccgct cacccgcccg ccggcgtgga tcagcagctg
     1621 tttgcgggcg gtgagctcac cctcgaaatg gccgtggatc tcggcgatgt cgatgccgac
     1681 cgtgcccttg aaggcgccct tttcggcgat gcgcaacacg cggctgtcca tcgtcgcctc
     1741 gacccgcccc tccacgacca gggtatcgca gtcgaggatc tcggcgccct tgagcttcac
     1801 ctcgggaccg acgatcagac ggctctcacc cacggccccc gcagccgccg ccgcatccgc
     1861 gcgctcgacc gcctgcggct cgcgcgctgc ggccgaaccg ccggtttcgg ccggcggcgt
     1921 cacgggcacc gcggcaacgt tcgtgcctgc cgtgcgttgg gcgttgcgct gggcggacac
     1981 ctcgccggtg ccgtggaaca gattgggctt gttgaacatg ttctctcctg gagtccggcg
     2041 cagcgacggc gctgcgctct ccagcccctt cagcaaccgc catgccaaag gaccttttcc
     2101 ttttgcctgc gggcacttgg cgcaccgacc cgggcggtac gtgggcgcac gcgcggggaa
     2161 accgtcgccc gtccccgaca ccgcatcccg agatcgggcg caagcctccg gggaacaagg
     2221 cctccccgct gtcgctcggc ggcgacaccg atacccgccg cttacttcgt cttgcacttg
     2281 cgcgacgctg cgaccgcgcg cgccgtccta gactcggcga ccccggcgca gcactccgct
     2341 gcgatgctca accccctccc tgcatgcatc acctctcctt tcgcagcatc ctgctcgcga
     2401 gcttcctgct gatcaccgcc agcctggccg ccgccgtcgc gggcggctgg cgcggcatgg
     2461 aaacggtcgt gcgcgcgatc caggaggaca agcagcgcgc gctcgccctc agcgccgcgg
     2521 cgcgtcagct ccgcgaacgc agcgtgaacc tcgaacgcag cgcgcgccag tacgaggtgc
     2581 tgggcgagcc ggcactcgcc cggcgcttcg aggccaccct cgccgaggca ctgcccgcgc
     2641 tcgcgctgct ggaatcggcc ggccccgcac tcgccagcgc aggcgcggcc tggcgcgcct
     2701 gcgcggaccg catcgccgca tggctggcga gcctcgccat acccgggccg accgacggcg
     2761 tgcgcgaaca gcccatgccc gccactgcgg ccgggcgcgg cgacggcctc gccacggcgg
     2821 acgacttccg ccacctcggc gcgctcgccg accggctcgc cgcggcgctg gaccaccatc
     2881 tcggcgagcg cgatctcgcc ctcatggaca cgctcgagcg cgaacgccgt gccgtcgccc
     2941 tgcagatcct cgccgccctg ctcctcgccg gcctgctcgc ggcactcgcg ggcgggtggc
     3001 tgctgcgccc gctcgggcgc atggaacagg cgatcggcga gctcggcgag aaccgcctca
     3061 cccgcccgat cgacatcgac ggccctgccg acctgcgcag gctgggcgag cggctcgact
     3121 ggctgcgcct gcgcctcgcc gaactcgagg ccgaccgcgg ccgcgtgctg cgccacgtct
     3181 cgcacgagct caagaccccg ctcgcctcgc tgcgcgaagg ggtcgccctg ctcgaggacg
     3241 gcgtgctcgg cccgctcgaa cccgagcagc gcgaggtgac cgccatcctc gcgcacagcg
     3301 cgcgcaccct gcaggcgcgc atcgaacagc tgctgcggct caacgcgagc cagttcgacg
     3361 cgcgctcgct gcacctgcag cccaccgccc tcctgcccct gctgcgcaag atcggcgagg
     3421 agcagcgcct gccggcgcgc gcacgccggg tgacgatcgg actcgcgggc gacgctccca
     3481 tcgtgcaagc cgacgccggc aagctgcaca gcgtgttctc caacctgctc gccaacgcga
     3541 tcgccttcag ccccaccggt ggccacatcc gcatcgagct ccgccgcgcc ggcgagaacg
     3601 tggtgatcga ctgccgcgac gaaggcccgg ggatcgcgcc cgacgagctc gaacgcatct
     3661 tcgagccctt ctaccagggt cgtcgcagcc ctccctcaag cgcaggcggc agcggcatcg
     3721 ggctggcgat cgtgcgcgag ttcatcggcg cccaccgcgg ccgggtgcgc gcgctgccga
     3781 gcgagcttgg cgcccatttc cgcgtcgagt tgccccatgc ctgacgcccg cctccacgtc
     3841 gtccgcgcag tctcccgcgc agcacgccgc gtgtgcgtcc ggatcgcccg cgccgtgctc
     3901 cccgccaccc tcgccctgct cgcggggtgc gccgacctcc cggcccggca ggcggccgag
     3961 gacgacacga gcgccgcggc acttgcccac gaagccgcgc agcagcgcct gcacaccgcg
     4021 gtgcaggccg cccagcccgg gcaggagcgg ctcgccacgg cgcggcgcgc gctcgaaagc
     4081 ctgctcgccg acgatcgcgc acaggcgcgc gcgctgcatc cctacgcacg cgccctgctc
     4141 gaacagctcc gcgtgcgcca gcaagtcgcc gcactcgccg agcacctgcg ccgccagctc
     4201 gacgagcgtg cccgcgccag cgacgcgcag gcgctggaac tcgacgcgct gcgccggcag
     4261 aacgccgcgc tgcagcgcca gctcgaggcg ctcgccgaga tcgaacgcga gctgacctcg
     4321 cccggcctgc cggtgcggcc ccgcaccggg ctgcccgaat gagcacgtcg acggagatgc
     4381 acgcgaccgc ggcggcggca ccggcgcgcc tgctggtggt cgacgacgac gccgacctgc
     4441 tgcgcctgct cgcgatgcgc ctgcaggcca ccggctacga cgtcgccacc gcggacagcg
     4501 tcgcggccgc acgcagccgt ctcggcgtgg agcgcttcga cctcgtggtg agcgacgtgc
     4561 gcctgcccga cggcgacgga ctcgccctgt tcgaggacat ccgccgccag catccggcgc
     4621 tgccggtcat cctgctcacc gcgcacggca ccatccccga tgcggtcgag gccaccgcgc
     4681 tcggggtcgc cggttatctc accaagccct tcgacagcca ggccctgctg cagggcatcc
     4741 gccaggccct gcaacgcgcc ggccccaccc acgcccgcag cggtctccgc gccgacgccc
     4801 ccggcagcgc cggcgacacg gcctggcgcg cagcgatcct cagccgcagc agccgcatgc
     4861 aggcagtcct cgacgaggcc cgtctggtcg ccgccgcgga cgccagcgtg ctgatccgcg
     4921 gtgacagcgg caccggcaag gagctgctcg cacgcgcgat ccacctcgcc agcccgcgcg
     4981 ccgctgcgcc cttcgtcgcg atcaactgcg gcgccattcc cgagccgctg ctcgaatccg
     5041 agctcttcgg ccacgtgcgc ggcgccttca ccggcgccac cagcgcccat cagggcctgg
     5101 tgcaggccgc ccacggcggc acactcttcc tcgacgagat cggcgacatg ccgcccgcgc
     5161 tgcaggtcaa gctgctgcgc gtgctgcagg agcgcgccgt gcgtccggtg ggtgcgacgc
     5221 gcgcgcaggc ggtcgacctg cgcatcgtct ccgccaccca ccgcgacctc gacgcggcgc
     5281 tggccgcggg ccagttccgc gcggacctct actaccgcat cgacgtggtg agcctgcagc
     5341 tgcccaccct ggccgagcgc cgcgaggaca tcccgctgct cgccgggcac ttcctgcgca
     5401 gcctcgcgca caagtacggc aggcgcctca ccggcttcgc gcccgaagcg ctggaggcac
     5461 tcgccaccgc gccgtggccg ggcaacgtgc gccagctcca caacgtggtc gagcaggcgt
     5521 gcgcgctcgc caccacggcg ctgatcccgc gcgccctggt cgaacgcgcc ctgcgcgtgc
     5581 agccggtcga ggccctcggc tacgccgagg cgaagcgccg cttcgagcga aactatctga
     5641 tacagctgct gaaactgact gcgggtaatg tttcggacgc tgcacggctg gcggagcgca
     5701 atcgcaccga gttctaccgc ctgctgcagc ggcacgacct gacgcccgag ctcttccgcg
     5761 gcgccggccc cgatgtcgaa caatgaacaa gacccagacg gagcccgaca tgatcaaacg
     5821 ccagaccatc gccgccatgc tgaccaccac cgcgatcgcc ttctcgatcg ccaccccggc
     5881 ccaggccgcc cgcctgctcg aagccggacc gttcgacacc gtctccgcct gcagcggtga
     5941 caagcccgcc accgctactc cgggcgagcg caaggacaag cccgcgcaga cctgatcgcc
     6001 gccggccctg cccgcttcaa atccaggccg cgccccgggc accgggtgcg cggccttgtt
     6061 cgtcagtgca tgggacggag gcgcggcggg ttcatgcagg aggcgcgaac gtggcggtcg
     6121 agctgcgccg ctccgctcag gcggtcccgc ccaccgtcag cccgtcgatg cgcagcgtcg
     6181 gctggccgac gcccaccgga acgctctgcc cgtccttgcc gcaagtcccg acgcccggat
     6241 ccagccgcat gtcgttgccg atcatgcgga tcttggtgag cgcgtccggg ccgttgccga
     6301 tcaaggtcgc ccccttcacc gggcgggtca ccttgccgtt ctcgatcagg taggcctcgg
     6361 cagtggagaa gacgaacttg cccgaggtga tgtccacctg gccgccgccg aagttcaccg
     6421 cgtacaggcc cttcttcacc gacttgatga tctcctccgg atccttgtcg ccgttcagca
     6481 tgtaggtgtt ggtcatgcgc ggcagcggca ggtgggcgaa ggactcgcgg cggccgttgc
     6541 cggtgaccgg catgcccatc aggcgggcat tcatcatgtc ctgcatgtag ccggtgagga
     6601 tgccgtcctc gatcagcacg gtgcgctcgg tcgggttgcc ctcgtcgtcg atcgacagcg
     6661 agccgcggcg atcgggcagc gtgccgtcgt caaccacggt gacgcccctg gccgccacct
     6721 gctggccgat gcgcccggaa aacgccgagc tgcccttgcg gttgaagtcg ccctccagcc
     6781 cgtggccgat cgcctcgtgg agcaggatgc ccggccagcc ggagccgagc acgaccggca
     6841 tggtgccggc cggggccggg tcggcgccga ggttcacgcg cgcctgatgc accgcctggc
     6901 gggcgtagtc gtgcaggcgg tcgtcgcaga accagccgta gtcgtagcgt ccgccgccgc
     6961 ctgcgctgcc ctgctcgcgg cggccgtcct cctccatgat cacggtgatc gacacgcgca
     7021 ccagcggacg cacatcggcg gcttgatggc cgtcgctgcg caccaccatc accacctccc
     7081 aggagccggc gatgtgggcc atgacctggg tcacgcgcgc atcctcggcg cgtgcgaagg
     7141 cctcgaggcg ctcgagcagc tcgaccttgg cggtgtcgtc gagcgagtcg agcgggtcgt
     7201 cggcccggta caggcgggtc ctgagcttgt gcgggacgat gccgacgcgg cggcgctcgc
     7261 cggccgcggc gatcgcacgc gtggcggtgg cggcgccgac cagggcgtcg agcgagatgt
     7321 cgtcggagta ggcgaaggcg gtcttctcgc cgctgatcgc gcgcacgccc acgccctgct
     7381 cgatgtcgaa gctgcccgac ttgacgatgc cctcttccag gctccaggcc tcggagcgct
     7441 ggtactggaa gtagaggtcg gcgaagtcga tgtcgtggcg catcagcttg cggaaggtct
     7501 tctcgagctc cgcctcgccc aggccgtagg gcgacagcag gtaacggtcg gcgaccttga
     7561 ggggattctg gctcttgctc atcggtaaat ccttggcgga ggatgccggt tggaccggca
     7621 tcgggcaaaa acgatcattg cggggcgacg acacgaggag gctgcggcat cggccacgtt
     7681 cgccccctcg cggacggcgc gttcaggcca ggcagcggtg gcgcaaggca ggcaggctcc
     7741 cgcgtaccgc cgcgaggcgc gcgggatcga ggtccgcgac caccacgccg gggccctcgt
     7801 cgcggcaggc gaggatctcg ccccagggat cgacgatcat ggtgtggccc caggtcaccc
     7861 gcccgctctc gtggcacccg ccctgggccg gcgccatcac gtagcactgg ttctcgatcg
     7921 cgcgcgcacg cagcagcacc tcccagtgcg cgcggccggt ggtccaggtg aaggcggccg
     7981 gcagcacgat cagatccacc tcgcccatgg cacggaagag ctcggggaag cgcaggtcgt
     8041 agcacaccga cagcccgatg cgcccggccg gcgagtcgaa gcaggcgacc gtgccgcccg
     8101 gctcgatggt ggccgcctcg tcgtagcgct cctcgccgcg ctggaagccg aacaggtgga
     8161 tcttgtcgta gcgcgccacg cgccggccct ggtcgtcgaa gaccagggtg ctgttgcgca
     8221 ccttgtcgtc ggcctcggcg accagcggga cggtgccgcc gatcagccac acgccgtggc
     8281 ggcgcgcctc gccggcgagg aaatcctgca gcgggccggc gccctcgggc tcgcggatgc
     8341 gcaccttggc ggtctcgtcc ttgctgatga gcggaaagta ttcgggcagc gcgatcacgc
     8401 gggcgccggc ggcggcggcc tgggcaatca ggtgcgacgc ggtttccagg ttggcggcga
     8461 cgtcgggccc ggagaccgtc tggatggcgg cgatgcgcac cggcgcggaa agggaggttt
     8521 cggtcatgtc ggcgagcttg gggcggggtg ctgccgctgg cggcaagacc ccgcgggggt
     8581 tgggcaagga agggcacgca gcttgggcgc tcgcccccgg atcagcgcga ctccggcgca
     8641 gcggccctgc cgcgcttggc gacctggggg tcggaccacg cgccggtcac cgtgtactcg
     8701 aaggcgaaga gcttctcgat cgggtcgctg agcaccttct gggcgagata ggtcaccacg
     8761 ccggcgaccg gattcaccaa gcccgccgcc gcgccgatcg ccaccgactc ggacagcgtc
     8821 ggctgcacca gaacgcgcag gtcctgcgtt tcggagacca cgtcggcgct gccgctcatc
     8881 gcgacgcgcg ccgcgggtcc gcggatctcc aggtcctcgc tgcgcagcac accacgggca
     8941 agctcgatgc taccggaaat gcgatcgaag gcgaatcctt cggagaacac gtcgcggaag
     9001 tcgagcgtga tccggcgcgg cagcgactgc aggctgagga tgccgagcag acggccgacg
     9061 ccgggttcga gccggttgaa ctggcccgcg ccggtctcca gccgcagcct gcccgacagg
     9121 ctgggatagt cgatccgggt cggcgcaccc cgccatgcga gctggccggc caggctggcg
     9181 gcgccgccgc gcaccacgtc ggggtggccc aggcgccgga tcagcccacc catcgacgcg
     9241 gtctcgagcg cgaagtcgag ctcggtgcgc tggcgcgcgc ccgcgcgcca ctgccccttg
     9301 ccggcgaggc gtccgtcggc gttcatcagc gcgaagcggt cgagctgcca gaggttgccg
     9361 cggttgcgtg cgaggacctc gaggcggccg agttcgagct cgttgatgtc gaagcgctcg
     9421 gccaccacgt cgagcgcggg caggcgctgc ggcggatcgt ccaccgcctc gtcgcccgcg
     9481 gagtcgccgt ctgcgccagc ctgcgcgccg ccggcctcgc gcaccagacg caggtggcgg
     9541 aagcgcgccg cgagcgtgcc cttgcccgcc gcccgccagt cgaactcgcc gtccgcgcga
     9601 tcgctctcca ggcgcgcctt ccagccgccc ggatcggcgc gcgcgcgcag gtccaccgcc
     9661 ttgaaggtct ggccgaacac acccacctcg tcggccgcga gcgcgaactc cgccaccgcg
     9721 gcgggcgctc catcgccggc cacggcagcc tcctccgcgc ccgcggcggg aaggggcgcg
     9781 gcgcgctcca gcacggcgcg ccaggcgtcg aggtcgaggc gatcgagcac cgcggcgaca
     9841 cgcacgcccg cctcggaacc cggcggcgcg tctgcgctcg cacgcccgag cgcaacgccg
     9901 ccgcgcacca ccccgccggc ggagcgcagc agctccgcgc gcaggcggtc gtcgatcgcg
     9961 agggtgatgc gcgcatcctg gctgccggcc ggacgctcga aagccagcct cgccggccag
    10021 ccctcggcgg ccgatttgcc gaacggcggc ggcaggctgc aggcgaaacc ggcaaggtcg
    10081 gaggcgatct cgacccgcgt gccgcgccgg ccgacgcgga tgtccgcgga ccactccgcg
    10141 gtgccggcga gttcgtcgag cagcgggacc tcccaggccg ccgccagcgc cgcggcgtcc
    10201 gcccgcccgg cggcgcggaa gcgcaccgtg ccgtcctcgc cggtctgcgc ggagaggcgc
    10261 aatggattgc cgaacaggcg cgcctgcgcc tccgggatgc tcagggtgtc cgcggtgaag
    10321 cgcagccgcc cgcgcgccgc ctcgagcggc ggcaaggcct ccagcagcgc gatgcggttg
    10381 tcggcgaagc ggtattcgcc ctgcacgcgg gagtcgatcg tcgcgcgcag cggcatcacc
    10441 agctcgagtt ccagttcgcc ccgcccctcg gccaccatgc cgtcggtgaa gccgtcgatg
    10501 cggcgcgaca ccgggctcgc cgagacgaag cgcaggaact cggcggtcgg accgcgcgca
    10561 cgaccggtga tggtcatgat ctcgctcgga agttggtcga ggtcgggcac ctccgccacc
    10621 acgtccacca gatccacccc gaagatgcgc ccgcgcggca cgaggatgcg cagacccggg
    10681 ccctcgaagc gcacctcgcc gtcgatgccc tcgatgcccg gccagccggg ggcgtagtcg
    10741 agcgcggcgt cggcaacctg aacgctcacc aggaagcggc cctggccggc acggaagggg
    10801 aagtcgtcga gcgcgccccg caattccagc cgcgcctcgg gcaccttcgc ggagcggatc
    10861 ccgcggcgca gccagtcgcg cgtgtcgtcg ttgaccgccc tgggcacgta gcgccacacc
    10921 gcggtcgact cggcgcgggt caggcgcgcc tgcagatcga tctcgcccgc ccccgtcgtc
    10981 gccggccagt aacggcccgc ggcggtgccg gccgcgtcgg cgttctcgaa cgcggcctcg
    11041 tcgagcacga tcgccagccg gccctccgcg cgctgccagc cgccctgcgc ctgcaggcgc
    11101 gagaagtcca gcggaccggg atcgaagacc gccggcaggt cgatccgcat gtccgccgcg
    11161 gcaagccgga agcgcccgcg ctgccggtcg ccgtccacac tgccggagag cccggtgacg
    11221 ccgggcagac cgtcttgcgc cgccagcccg agcccggcga agcttgccgt cagcgcccag
    11281 tgctcgggcg cggcgggctc ccccttccag gcgaggcgca ggccctcgac ccgaccgcgc
    11341 ggcgcaagcg ccgccaggcg cgagcgcagc ccggcatcga gcggcaggtg ggcggcgagc
    11401 gcgacgagcg tggccagttc gagccggtct gcgacgaggc gccccccggc cgccgcgggc
    11461 ccgcgcccgg cctgctcctc gccggtgcca cgatcgcgcg ccttgccatc gagcgccgga
    11521 tccagcccca gctcgacatc ggtggggggc agcacgaggc cctgggcggt ttcgaacccg
    11581 agcccgcggc cggcgaagcg gaatccatcc tcgccgcggg ccagctcgac acgaccgtcg
    11641 agacgggcga gctcgagaac cggcagatcc tcgccgagca cggcccgcac cccgtccagc
    11701 gcgaggtcgg cggtcagcgc atgaccgcca cggccgtccg cgtcgagcca ggcttgcagc
    11761 gcaccctcgc cttccaggcc gaaaggcagg tcgacccagg gccgccagtt gcccagcgcc
    11821 gtgcgcccga gttcgacgta caaccgaccg gcgaaacgct gcgggcgcag cgggtcgatg
    11881 cggcgcagct cgccgcgcag gctcgcacgc tcgccgaggg cggccggcag ccgtgcctcg
    11941 agcgcgaagc gcgccggacg cgtgcgtcga tcgaggcgga attccacctc ttcgagctcg
    12001 agcaccggcg caccgcgcgc ttcgtccgcc caggcaagac gcgcgccgtg gatgaggatc
    12061 tggcgctgcg cgagcagcca gccgaggccg ccgtcgccgc cctcgtcccc gtgtgcaagc
    12121 gcgatgcctg cgacgtgcag cgctccctcc ggatcgcggc gtaccaacag gtccggcgcg
    12181 cggatctcga ggcggtggaa atgcggctcg agcatcgcca gcgaggacca cgccagggtg
    12241 gcgtcgatct cgggcagggt cagcgcgggg cggccctgcg cgtcggacag gctcaccccg
    12301 gccaggcgca ggcgcggtcg ccagcccgcc cagccgccct cgatgcgctc gatcgccacc
    12361 gccacgccaa ggcgctcgcc gagcaactcg gcgatcggca cgcgcagttc gcccactgcc
    12421 ggcagcacga gatggcgcag caccagcacc ccgaccgcga gcacgcacca gaccagcagc
    12481 agcgcatcgg acagcctgcc ggccgcgcgc cgccagccgc cgcgcgcggc cggcaggccg
    12541 tcgacggacg ggggtacgga ttcggcaggt gcgtgcgggg cggaggggct cagtcggatg
    12601 ctcgcaggac gttgtgtggg agcgcggttg cagcgctgcc ggcgcatggc gcgggcagct
    12661 agaatattgg gatcgcgccc gccaccggat cgccgacgcg cgcgcattct accaagtctc
    12721 gatcgccctc tcgatcaccc tctcgcgccg cactggagcc ccctcatgtc cgccccgaac
    12781 agcgtttccg cccctgccct cccgcccgcg gtggcgcagg cgcgccgcct gtcgcgctat
    12841 ctggcgcgca tgctcgacag ccagccctgg ctggcggagg cgctggccgc cagcatcgag
    12901 cgcccgctcg ccgaggccga cctgcagcgc ttcgtggccg cgcgcgaggt cctccacggt
    12961 ggcggcgacg gcgcgctgcg cccggcgctg cgccagctgc gcatgtggac gctgtgccac
    13021 ctgatcgtgc gcgacctcgc gctcggcgcc ccgctcgccg aggtcaccga gaccatgacc
    13081 ctgctcgccg aggtggcgct gcgccacgcc cacgacgtgc tgcgcgcggg attggtcgag
    13141 cgccacgggc agccgctctc gcccaccggc tgggagcagg agctcctcgt ggtcggcatg
    13201 ggcaagctcg gcgggcgcga gctcaacgtg tcctccgaca tcgacctgat cttcatctac
    13261 ccggaggacg gcgacaccgg cgggcgcaag gcgatcagca actacgagtt cttcgagaag
    13321 ctcggcaagc agctgatcca ggcgctcgcc gagatcaccg agcacggcca ggtcttccgc
    13381 gtggacatgc gcctgcgccc gaacggcgac tccggcccgc tggtgtgctg cttcgacatg
    13441 ctggagaact acttcgtcac ccagggccgc gagtgggagc gctacgcctg gatcaaggcg
    13501 cgggtgatgc tgggcgagcg ctggcccgag ctcgaacagc tcgcgcgccc cttcgtgttc
    13561 cgcaagtacc tcgacttcgg tgcgatcaac gcgatgcgcg agctgcacgc gcagatccgc
    13621 cgcgaagtcg cccgccgcga gcgcgccaac aacgtcaagc tcggtcccgg cggcatccgc
    13681 gagatcgagt tcatcgccca ggtcttccag ctcatccgcg gcgggcgcga caccgcgctg
    13741 caggtgcgac cgacgctcgc ggtgctcgcc cgcttgcccg agcgcggcat cctttcggag
    13801 gaggcggtga cggagctgtc gaacgcctac gtcttcctgc gccggctcga gcaccgcctg
    13861 cagtacctcg acgacgccca gacccacgac ctgcccgaga aggacgagga tcgcgcgctg
    13921 atcgccgagg ccatgggctt cgccgactac gcggcgctgc tcgcggtgct cgacgcccac
    13981 cgcgaggtgg tgagccggca cttcgacagc gtgttcggcg acccctccga ggaggaccac
    14041 agccttgatg ccacctgggc caacgcgcgc gacaccgagg ccgccaccgg cgcgctcggc
    14101 gagctcggct accgccaccc gcgcatggcc gccgagcgtc tcgccgcgat gcacgccagc
    14161 ccgcgctatc gccagctgcc caacaacatc aagagccgct tcgacgcgct catgccgcgc
    14221 gtgctcgaag ccgccgccag cacgccgggc gcggacgaga cgctcgcgcg catgctcgac
    14281 ctcatcgagg cgatcggccg ccgcggcgcc tatctcgcgc tgctgcagca atacccgcag
    14341 gcgctgcgcc gggtcgccga cctgatgagc gcatcgcgct ggggcgcgca gttcctcact
    14401 cgccatccga tcctgctcga cgagatgctc gacgcgcgca gcctggacaa cgcccccgac
    14461 tggaaggcct tccgcgccag cctggccgag gagctcgagg cgctcgaacc cgacatggag
    14521 cgccagatgg acgtgatgcg cgagcgccat cacgcccagg tcttccgcct gctcacccag
    14581 gacctcgccg gcctgctcac ggtcgagaag ctcgccgacc acctctccga gctcgccgac
    14641 atcgtgctcg acctcaccct gccgctgtgc tggcggcgca tcaagatccg ccaccgcgag
    14701 gcgccgaagt tcgcggtgat cagctacggc aagctcggcg gcaaggagct cggctacgcc
    14761 tccgacctcg acatcgtgtt cctccacgac gacgaggcgg gggaggcgcc cgaggtctac
    14821 acccggctgg cgcagcgcgt cagcacctgg ctgtcgagcc agaccgccgc cggccagctc
    14881 ttcgagaccg acctgcgcct gcgcccgaac ggcgagtccg gcctgctggt gagctcgctc
    14941 gagtccttcc gcagctatca gctcgagtcg gcctgggtgt gggagcacca ggccttgacg
    15001 cgcgcgcgtt tctcggccgg cgaccgcacg atcggggaag ccttcgagcg catccgctgc
    15061 gaggtgctgt gcatgcggcg cgacctgccc agcctgcgcg ccgaggtgct ggcgatgcgg
    15121 cacaagatgc gcgacgcaca cggcagcagg agcgaggagg tgttcgacct caagcacgac
    15181 gaaggcgggc tgatcgacgt cgagttcctg atccagtacc tggtgctcgg ccacagccac
    15241 gaccacccac gcctcaccgg caacctcggc aacatcgcgc tgctgggcat cgccggcgag
    15301 ctcggcctgg tccccgccga cctcgccgcc gcctgcgccg acagctaccg cgaactgcgc
    15361 cgcctgcaac accgccagcg cctcaacgag cgtccctccc gcgtcgatcc cgccgaggcc
    15421 gcggccgcac gcaagccggt gcgcgcgctg tggcgggccg tgttcgggga ctaggccgcc
    15481 cggctgcgca gcgctttccc gtgggagcga cgcgagtcgc gatcgaccgt cgagcggcgc
    15541 gagacgccag cgggcaccga tccgctgcag gcccagcgcg ggaaagtgaa atatttcacg
    15601 tcgcaaaagc gcgcatgacg ccccgcgggc cggcgtctag aatccagggc acctgtcttc
    15661 cagcgcccgc ccgcgggcga cctccatgag ccacgaaatc gaactcaagc tcgcgctgcc
    15721 caggcgcgcg ctcccggcgc tgcgccgcca cccgctggtg gccgcggcgg agaaatgcgg
    15781 caacgccctc accctcgaca acacctacta cgacaccccg aagctgcagc tcaaggcgcg
    15841 caaggtggcc gtgcgcaccc gccgccaggg ccgccagatg ctgcagacgg tgaaatgcgc
    15901 ggcggtatcg agcggcgggc tgtcgcagcg gccggagtgg gagaccgcgt ggaccggcgg
    15961 cttcgatttc acgccggtcg acgaccccgc caccgcgcgt ctgctcgaac gccaccgcgc
    16021 agagctggtg ccggtgttca ccacccgctt ccgccgcgag acgcggcgac tcgtcccgca
    16081 ggcgggcgtg tccatcctgc tgatgatcga caccggcgcg gtgcatgtgc gcaccccgga
    16141 gggcgtcgag cgcgaggcgg agatctgcga actcgagctg gaactcgaga gcgggcgcgc
    16201 gcaggacctc ctcgacctcg cctgcacgct cgcgcaggac ctgccgctga tgccggccga
    16261 cctctccaag gccgaacgcg gctaccgcct tttcctcgac acgcccgcgg gcgcgctgcg
    16321 ctccgagatc tccacgctcg agcccggcca gaacgtggtc gaggccttcc agggcctggc
    16381 actgtcctgc gtgcgccagt ggcagggcaa cgccgcgacc gcgctcgcgc agggcgaccc
    16441 cgacgcgatc gaccccgaca acatccacca gctgcgcgtc gcccatcgcc gcctgcgcgc
    16501 gctgctcaag atcttcgcac ccgccctgcc cgagaccttc gccggcacct ggaacgcccg
    16561 cctgcgcgac aacgccaacc gcttcggcga tgcgcgcgac ctggatgtgt tccacgccga
    16621 gctgctcgag ccggtgaccc ccgagggcct cgccgacgca gcctcgatgg cggccctgct
    16681 cgagaccgtg cgcacggcgc gcgcaagcgc ccgccaccac gccggcgtca gcctcgacct
    16741 cgccacccag ggccggctgc tgctggagtt caccgccgca ctccaccgcc tgcgtgccga
    16801 cagcctcgcc gaagccgccg acctgcgcag cttcgcccgc ctgcgcctcg accggctgcg
    16861 caagcgcgcc cgccgcggcg cccgggcggc ggccagcctg gagcccaccc gcctgcacgc
    16921 gctgcgcatc gacttcaaga tgctgcgcta cggcgtcgag ttcttcgcgc cgctgttcgg
    16981 caccaggagc atcacccgct atctcgacgg cgtggtgcgc gcgcagacca cgctcggctt
    17041 cctgcaggac gtcgacaccg cgcaccagcg cctggccgac tggtcgcgaa cgcagcccgc
    17101 gctcgccgcg gccgcggcct tcgtgctcgg ctggcacgcc ccgcgctacg cccgcctgcg
    17161 ccgccgcgtg ctgcgcgagt gcgagccgct gctgtggggc ggcaagccgt ggtgagacgg
    17221 cgccccacgg cggcggcgcg acagaccgcg gtcatcgtgc tgtaaccgca gccgccgatg
    17281 atgcgcgccc tcgaaccccg gatgaacgac atggacctgc tgctctggcg tcacgcggaa
    17341 gcggtggacg gcagccccga ccacacccgc gaactcaccc cccgcggtct caagcaggcg
    17401 cgtcgcatgg cgcgttggct ggaagacaac cggccgaaga acctgaaggt actggtcagc
    17461 cccacgctgc gcacccgcca gaccgcgacc gccttcaccg acgacttcga gatcgtcggc
    17521 ggcctgggcc cggacggcaa cgtcgccgac atcctcgccg ccaccggctg gcccgagggc
    17581 aacggcgccg ccctggtggt cggccaccag cccgcccttg gccgtctgtg cgcgctgctg
    17641 ctgtcgggca gcgaggccga ctggacgatc aagaagggtg cgctgtggtg gtttaccaac
    17701 cgcgtgcgcg aggacgagac gcagacggtg ctgcgcgcgg tgattcccag cgagctggtg
    17761 taaggcgcca ggcgtgagga gcgcggggcg ccccgctgct cacgcctggc gctcccattg
    17821 aattcctcgc cgcgagcacg gtccaacccg tcgtgaaata tcccggacgg caggctcgcg
    17881 cacggtcctc gaagcccgtg gagggctcgc ttgtgctttg cgatgcaatg cgtaagatgc
    17941 cgtcttcctt ccggaacgga ccatgctcga acgctttctg cccactcgcg aggccatcac
    18001 gcaaagtcgc atgctgcgct ggctggggcc gcgcctgcac gacccgctgc tgtggcacgt
    18061 caaccggcgc tcggtggcac gcggcgtggc gatgggcgtg ttcttcgggc tgatgatccc
    18121 gatcgcgcaa atcccggcgg cagcgatcgc ctcgctgctg ctgcgcggca acctgctgat
    18181 cgcggcagcc tccacgctgg tcagcaatcc gctcacctac ggtccgctct actacttcgc
    18241 ctaccagctc ggcgcgggcg tgatcggccg ccgcacgcca gccgcgctca ccgcggacga
    18301 cgtcgaggcg ccggtgcgcg ccatcgactc gctcgcccag gcctgggcct ggatcggcgg
    18361 catcggccag ccgctgctgg tcggcatggt gatcatggcg gtgacgggcg cgctcgtcgc
    18421 ctactggggc acgcaactgg tgtggcggct ccgcgtcgcc gcccgtcgtc gccgccagcg
    18481 ccgcgagcgc agcgcgcgcg catccgcgca ggctattgga agcgcctgaa cgcgggcgta
    18541 tccttcgcat cccctcatcc gcccaacgga cggccatggc catggaagag tgcatccccg
    18601 acggtacggt cgcgatcatc gacggcgtcc gccgcatcta ctacgacggc tactggctca
    18661 aggcctacga gccgccggcg gacacgctcg acgccaagaa gcggctgatc caggcgctca
    18721 cccggcgcct gttcaaccat gtcgagcacg gcatcaacat ccccggcaaa cgcctcgacg
    18781 atgcgcgccg cgcctacgag accgaggagg accccgcgcg caagcgcgtc aagggcgcga
    18841 tgtacgcggg cgcgctgttc aaccgcgcca ccgacatctt caccaagctg gtggagttgc
    18901 aggagctggg catcgagatc gccaccgaca acccgctcat gcgcgaatgc ggcggctgcc
    18961 tgcaggaggc gctcaagctc ggccgcctgg tgctgcaccg cagcggcgac gagggcatcg
    19021 acgagctgtg gggcgaaccc ttccgcgcct tctcgatccc ggtcgaggcc ttctacgaga
    19081 gccgctacat caagatcgcc caggcgctgc gcgaggtaga ccgcatcgcg gccgtgatgg
    19141 cgctcacctt cgccgcgacg ccgctctttc gcggcgtcga gcccctcgtg ttcgagttcg
    19201 cgcgcatcgc caagatcaag tgcgagacct tgcgcaccga cccggacatc ttcgacgtgt
    19261 gggcggagtt cgtcaccgcc tcggagcgcc tcgccgcgct cacgccggcg ctcggcccgg
    19321 gcgccgacga gtgcacccgc cagctcgccg ccgacggcct gcacctgatc ctgcaggggc
    19381 gcaacctggt caccgacatc atccgcgcac gcgtgaccat gcccaagagc gcgcgcgagt
    19441 tcatcgagcg ctgcgagacc ttcgcggcac gcgcgcaggc ggcctcgcat cccccgctcg
    19501 ctgccgccaa cgtgggcctg ggcgagacgc gcgcgtaaac aaacagcgcg cgcgggggcg
    19561 tgcgcggaga agcatcgcga cttgcgtcgc tcctacagga gaggccgctc gcgcctcaca
    19621 ggatgatccc gccgcccagg cacaccttcg actcgtagac cacgacgctc tgcccggggg
    19681 tcaccgccca ctgcggctgg gcgaaggcga tctcggcgcg gccgtcggcg atgcggtcga
    19741 tctcgcaggg ctggtcgggg gtgcggtagc gtggcttggc ggtgtacacc cagtgggagt
    19801 gcgggtcgcg tccggcgatc cagttcagct cgccggcgac caggtggtcc ctgagcagcg
    19861 cggggtgggc gtggccctgg accacgtaga gcacgttggc cttgacgtcc ttctgcgcca
    19921 cgtaccaggc ctcgtgttcg cccgcctcgt cctggtggcc cttgaggccg ccgatgtgga
    19981 ggcccttgcg ctggccgatg gtgtggtaca tcaggccctg gtgctcgccg agcacgcggc
    20041 cgtcgtcgag cgcgcggatc tcgcccggcc gggtcggcag gtagcgcatc aggaactcgc
    20101 ggaaggggcg ctcgccgatg aagcagatgc cggtggagtc cttcttctcg gccacgtgca
    20161 gcccggcctc ggcggcgatc cggcgcacct cgcgcttgta cagcgcgccg agcgggaaca
    20221 tcgtcttggc gagctgctcc tggttgagcc ggtagaggaa gtagctctga tccttggtgc
    20281 cgtcctcggc cttgagcagc tggtactcgc tgcggccgtc gttggcccac tcgcgcacct
    20341 gggcgtagtg gccggtggcg atgcgttcgg cgccgagctg catggcgtgg tcgaggaagc
    20401 agcggaactt gatctcggag ttgcacagca cgtccgggtt gggcgtgcgc ccggcctcgt
    20461 actcgtgcag gaagtcggcg aacacgcggt ccttgtattc ggcgctgaag ttgaccacct
    20521 cgaggtcgat gccgatcacg tcgcacaccg aggccacgtc gacgaggtcc tgccgcgtcg
    20581 agcaatattc cgaatcgtcg tcgtcctccc agttcttcat gaacagcccg gtgacctggt
    20641 agccctggcg tttcagcagc agcgcggtca ccgccgagtc caccccgccg gacatgccga
    20701 ccaccacctt catccccgcc ccgtcctgct ttccatgccg actactggac attcggtcca
    20761 cctccattga gccattccgc gagcggcagc acgaccgccg gctcgccatt gtcgacgaac
    20821 cagctgtcga ccacgtaacg ttgtcccgga cggtcgtcgc cgtccatctc tccgatcacc
    20881 agcccgtcct cggtgcagtt gcagcccgcc aacgcctggc cgggcggcag gcgctcgaag
    20941 cgctcctcca ccgacagcgc ttcgatcacc gcgctgaaat gctgcaggat cagccgggtg
    21001 cggcgcgccg gttcgaccac gcgatggaag cgcagcgcgc cgcgcgcctc gagcagctgc
    21061 agcagccgcg tcgtggaggt cgagtgatcg atgcagtcca tgcgcccgtc cgcgccctcg
    21121 tcgagcaggt tgccggcgcg gtcggccgcg atcgccgtct gggtcgcggc cacccggtac
    21181 agcccgccca ccgcctcggc gaggcgcgcg cgctcctccc cggcgctgcg tgccgcagcg
    21241 aggcgttcgc cgatccggcg cagggtggac tcgcgcacgc gcacgctgcc ctcgctcgcg
    21301 cagccgtagt tgtagcagat gttcacgcgc gcgccgccgg cggggccctg cgcccccgcc
    21361 atcgcccacg tcgtcgccgg cagggcgagc agcgcgggca gcagcaggcg gagcgcaggt
    21421 gccatcatcc cgttcagcgg tgatgcacca gcgccagcgg atggcgctgc ccggcgatcc
    21481 agtcctcgat gcactgcagg atcagcgggc tgcggtggcg atcggcgcat gcgcgcacct
    21541 cgtcgagcgt cagccacacc gcgcgcacga tgccctcgtc cagcctgcgc ccggactcct
    21601 cgccgcacag acggccgccg taggcgaagc gcaggtaggt gatgtcgccc tgggggcggg
    21661 tccactgata gatgccgacc aggtattccg gctcgaagtg gtgggcggtc tcttccagca
    21721 cctcgcgcac cgtggcctgc agcagggact cgccctcctc gaggtggccg gcaggctgat
    21781 tgaagcgcag gccttcgggc gtctcctcct cgacgagcag gaagcggccg tcgcgctcga
    21841 tgatggcggc gacggtgaca ttgggtttcc agggacgatc cttcacttcg atgctccagg
    21901 cgcatgaagg cccgcattct agcgcgcacc accggggatg gaggagcacc gcttgcccgt
    21961 cgaccgcgct ggcgcccggt ctgcgacggc ggtcgcggct ttgcgcgcgg cccgcgtcct
    22021 cggcacggct ttacgctaga atccgaaggc tttgcgcccc gtggacgatc gagggtgcgc
    22081 gacgaaccca tccagcgaac atttttcaag gcggagatct tcatcatgtc catggctgac
    22141 cgcgacggct tcatctggca ggacggcaag ctcgttccgt ggcgcgaggc gaccacccac
    22201 gtcctcaccc actcgctcca ctacggcctc gccgtgttcg agggcgtccg tgcctacaac
    22261 accgtcagcg gcaccgcgat cttccgcctc gccgagcaca cccagcgcct gatcaattcc
    22321 ggcaagatct acatgatgga catcccctat tccaaggatg aactcatgga ggcgcagaag
    22381 gaggtcgtgc gcgcgaacaa gctcgagtcc tgctacctgc gcccgatcgc cttctacggc
    22441 tcggaaaaga tgggcatctc cacgctcggc gcgcgcgtcc atgtcgcgat cgcggcctgg
    22501 ccctggggcg cctacctcgg cgaggaaggc ctgcagaagg gcatccgcgt gaagacctcg
    22561 tcctacaccc gccaccacgt caactcgacg atgccgcgcg ccaagctgtc ggcgacctac
    22621 ccgaactcga tcctcgccaa cctcgaggtc acccgcatgg gctacgacga ggccctgctg
    22681 ctcgacaacc agggcttcgt cgccgagggc gcgggcgaga acctcttcat cgtcaaggac
    22741 ggccgcatct acgagcccga gatcgcctcc gcgctcaccg gcatcacccg cgactccatc
    22801 cacgtgatcg cgcgcgaact cggctacgag gtcggcacca agcgcctgac ccgcgacgac
    22861 atctacctcg ccgacgaggc cttcttcacc ggcaccgccg ccgaggtcac cccgatccgc
    22921 gagctcgacg accgccagat cggcgaaggc aggcgcggcc cggtcaccga gaagatccag
    22981 acccgcttct tcgacgtcgt caacggccgt gcgcccgaat acgcgcactg gctcgcccac
    23041 gtctgatttc cacgctccga ggattccgtc atggttgaga acaccgacaa gacccaggcc
    23101 gtcgtcacca ccgccaagga cctgccgctg cactgcccgc cgccgggcgc gccgctgtgg
    23161 gcccgccacc cgcgcgtgtt cctcgacatc ctgaagaccg gcgaggcggt ctgcccgtac
    23221 tgcagcgcgc actacgtgtt caacggcgag cgccccaagg gccatcactg agaacccggg
    23281 tgccgggccg gaatggcccg cgacccgacc ggaccacggg ggctgcggcc cccgtggtct
    23341 ttacgatcca cccacaataa caacacaacc ttgagcagac ctgtcttcac caccgccctc
    23401 gaagcggaag ccgccttcta ccgcgcactc tccgagggcg acttcgaggc cttgatggcc
    23461 gtctggtcgg aagaggagga ggtcgtctgc gtccagccgg gcggcccgcg catcgtcggc
    23521 ctggccgcgg tgcgcgagat ctggcggcag atcctcgccg acggcgcccg catccgcatc
    23581 gacatcagcc atgcggtgac cagctccacc gcgatgatgg cgatgcactg cgtgctcgag
    23641 cgcctcgcca ccgacggtcc caccccgcgt accgcgacca tcgccgcgac caacgtcttc
    23701 gtgcgcggcg ccgacggctg gcgcatggtg ctgcaccacg cctcgccggt gcccgagatc
    23761 tcgggtcccg aggattcgac gccgcgcgtg ctgcactgag cccgcccgca cacggcgact
    23821 cgtccgcggc ggcgtgaccc ggtcgcgctt cgcggcctag aatcgcgcca accgccgccc
    23881 gggccggcat cgccgggcga gcttccccgc cttcgccctg cctggaaccg cccatgcgcc
    23941 accccctgcc cgcagcagag cttttcaagg cctacgacat ccgcggcatc gtcgacaccg
    24001 tgctgactcc cacggcggtg cgcgcgatcg gccacgcgat cggctccgaa gccgcggtgc
    24061 gcggccagcg cgcgatcgcg gtgggtcgcg acggccgcct gtccggcccg gcgctggccg
    24121 gcgcgctgac tgcgggcatc cgcgccgccg gcatcgacgt gatcgacatc ggctgcgtgc
    24181 ccaccccgct cacctacttc gccgccttcg agctcggctg cgcctcctgc gtgtcggtca
    24241 ccggcagcca caacccgccc gcctacaacg gcctcaagat cgtgctcgac ggccgcaccc
    24301 tgcacggcga ggcgatccag gacctgcgcc ggcgcatcgc cgaggatgac ctcgtcgagg
    24361 gtgccggcgc gctgcgcggc gccgacgtgc gcgccgccta cctcgaccgc atcgtcggcg
    24421 acatcgagct cgcgcgcccg ctgaagatcg tgctcgactg cggcaacggc gtggccggcg
    24481 ccgtcgcgcc cgaactcttc cgccgcctgg gctgcgagct cgtcgagctg ttctgcgagg
    24541 tcgacggccg ctttcccaat caccaccccg acccgtcgcg gccggagaac ctgcaggacg
    24601 tcatccgcgc gctgcgcgag accgacgccg agctgggcct ggccttcgac ggcgacggcg
    24661 accgcctcgg cgtggtgtgc aaggacggcg agatcgtcta cccggatcgc cagctcatgc
    24721 tcttcgccgc ggacgtactc gcccgcgtac cgggcggcga gatcctctac gacgtcaagt
    24781 gcacccgcaa tctggcgccg tggatccgcg cgcacggcgg ccggccgacg atgtggaaga
    24841 ccggccacgc gctgctcaag gcgaagctgc aggagaccgg cgccgcactc gccggcgaga
    24901 tgagcggcca catgttcttc aaggagcgct ggtatggctt cgacgacggc ctctacgcgg
    24961 gcgcccgcct gctcgagatc ctctccgccc gcgccgacgc caacgccgcg ctgaaggcgc
    25021 tgcccgacgc gacgagcacc ccggagctga acctgaagat gcaggagggc gagcccttcg
    25081 agctggtgcg ccggctgcgc gaggccgcgc tcgccacgcc ctcgtgcttc gccgaagcgc
    25141 gcgaggtgat cacgctcgac ggcctgcggg tggagttcgc ccacggcttc ggcctcgccc
    25201 gcccctcgaa caccacgccg atggtggtgc tgcgcttcga ggccgacgac gccgcggcgc
    25261 tcgcctcgat cgaggcggcg ttccggcgcg cgctcgacgc agtctggccc ggactcgcgc
    25321 tgccgttctg aggccacgga cgagcgcccg cgcgcacgcc cgccgggcgc cgaccggccc
    25381 gcccatccgg catgggcgcg cgcggcacgc ggcgatccgg gcaagcggac agcaaacgcg
    25441 aaaaaaagca cgctgctcac atcagttgca gcgcacaatc cgaagattct ttttacccaa
    25501 tccaaggcga gcgaggcagt ccatgacggc acgggaacag cgcgaagcac gcgcacgcga
    25561 cagcaggcag gcccagaagg gcatcgacat cccgtcctgc cccggcatcc tcgcccagct
    25621 catggacgag ttgcgccagc cgcaggtcag cggcaagcgc atcgccgcgc tgatcggcca
    25681 ggacgtcggg ctggccgcgg tggtggtgcg ctcggccaat tcgccctact tcggcagcgg
    25741 gcggcggatc gcctcgatcg acgacgcaat ccgcatgctc ggcttcggca tgctcgccaa
    25801 cctcgtccag gaggcgttac tgcgcagcaa catcgccgac cccggcgccg cgctcgaacg
    25861 cttctgggac agctcgcgct gcaccgcgca ggcgagcgcg atgctcgccc gccgcaccgg
    25921 catcgcgcgc ccggagaccg cctacacctt cggcctcttc cacgactgcg gcatcccgct
    25981 gctggtcagg cgcttccccg aatacaagcg catcctggcc acggccaacc aggccaccgg
    26041 gcgctggttc accgatgtcg aggacgaggc gctcgacacc aaccacgcca ccatcggcta
    26101 cctgctcgcg cgctcctggg gcctcaccga cgcggtgtgc accggcatcc tgtgccatca
    26161 cgactactcg gtgttctccc ccgactccgg cctggaggtc gaggcgcaaa ccctgatcgc
    26221 gctcaacctg gtggccgagt acgtcgccgg cacccacctg cgcacccggc gcgatgccga
    26281 gtgggacaag gggcgcgagg cggtggcgcg cttcctgggc tacgcgccga ccgagctcga
    26341 cgacctcgcc gacgacctgc tctacgcgct cgaccgccaa ggcgaggacc tcccggcctg
    26401 agccccgcgc gggcggcccc ccggcaggtg cccgccgctc acaccggctc gagcttggcc
    26461 acgccgagca gcagccactt gacgcccgcc ggaccgaagt tgatctgcac cttggcatcg
    26521 ctgccgctgc cttcggcggc aacgatcacg ccctggccga atttcgcatg cgccaccgcc
    26581 tggccgatgc gcaggccgcc gggcagcgtg gcgaaggccg ggcgcggcgc gcgctgcacc
    26641 tgcggcgtgc tccccaccgc ggacttggcc gagggcttga agtagccccc gcccatcccg
    26701 cccatcgcgc cgccggcggc cgagcgcggc tggggcgggg tgagccattt ggtcagcccg
    26761 gcggggatct cctccaggaa gcgcgagcgc aggttgtagc gcgtctgccc gtgcagcatg
    26821 cggctctgcg cgaaactcag gtacaggcgc tcgcgcgccc gcgtcaccgc gacgtacatc
    26881 aggcggcgct cctcctccag cccgtcctgc tcgaggatgc tgttctcgtg cgggaacagg
    26941 ccctcctcca gcccggacag gaagaccacg tcgaactcca ggcccttggc cgcatgcacg
    27001 gtcatcagct gcaccgcgtc cgcgccctgg tcggcctggt ggtcgcccgc ctccagcgag
    27061 gcgtgggcga ggaaatccgc cagcagcgcg tgcggctgcg cctcgccggc gcccgcctgg
    27121 gcctgcgcct cggcgaggaa gttggccgcg gcgttgatca gttcgtcgag gttctcgagg
    27181 cgctcgcggc cctccttgtc ggccttgtag tgcgcgcgca gcccgctcag gtcgagcaca
    27241 tgatcgacca gctcgggcag cggcaggccg gcggtgtcgc ggcgcagctc ctcgaccagg
    27301 cgcacgaaga ccgcgagcgc gctgccgggc ttgccggaca gatgcggcac ggtggcgtag
    27361 agactggtgt tgtacgtgcg cgcggcgtcg gccagcacct cgagcgagcg cgcgccgatg
    27421 ccgcgggtgg gaaagttcac cacgcgggcg aacgaggtgt cgtcgtcggg gttggcgatc
    27481 aggcgcaggt aggcgagcgc gtgcttgatc tcctggcgct cgaagaagcg cagcccgcca
    27541 tacacgcggt agggaatgcc ggccgcgaag agctggtgct cgagcacgcg cgactgcgcg
    27601 ttggagcggt agagcagcgc gatctcgctg cgcagcgcgc cgtcgcgcac cagggcctgg
    27661 atctcctcga cgatccagcg cgcctcgtcg gcatccgcat aggcctcgaa gacacggatc
    27721 ggctcgcccg cgccctggtc ggtccacagg ttcttgccga ggcggttgct gttgtgaagg
    27781 atgatcgcgt tggcggcgtc gaggatgttg gcgtgcgagc ggtagttctg ctccaggcgg
    27841 atcacgttgt tcacccggaa ctcgcgctcg aaatcgcgca tgttgccgac ctcggcgccg
    27901 cggaagcggt agatgctctg gtcgtcgtcg ccgacgcaga acatcgccgc gccgccctcg
    27961 cggccctcgt cggcgaagag cttcaaccag cggtactgca ggcggttggt gtcctggaac
    28021 tcgtcgacca ggatgtggcg gaagcggcgc tggtagtggc ggcgcagcgg ctcgttgcgc
    28081 tcgagcagtt cgtggctgcg cagcagcaac tccgcaaaat ccacaacaga ctcgcgctgg
    28141 cactgcgcct catactcctg gtagagctgc acgcgcaggc gggtgtagtc gtcccaggcc
    28201 tcgaccacgt gcgggcggtt gccggcttcc ttctggccgt tgatgaaatg ctggagctcg
    28261 cgcggaggga acttctcgtc gtcgacgttc aaggtcttca gcaggcgctt gatcgcggcg
    28321 agctggtcag ccgagtcgag gatctggaag agctgcggca acccggcgtc gcggtggtgg
    28381 gcgcgcaaca ggcggttggc caggccgtgg aaggtgccga tccacatgcc gcgggtgctc
    28441 accggcagca tcgcgccgag gcgggcgagc atttccttgg cggctttgtt ggtaaatgta
    28501 accgcgagga tgccggccgg gtcgacttgg ccggactgga tcagccaggc gatgcgggtg
    28561 gtgagcacgc gcgtcttgcc cgagccggct ccggccagga tcagggcgtg ttgcgcgggc
    28621 aggctcaccg cctgcagttg ttcggggttc aggttgctca gcagggtgga catcgcgttt
    28681 cctcttcgag ccggcattgt atcgaccccg ggggacgagg ccgatacggc aggccgggga
    28741 cagcgcggag agaaaaggac acaacatgat gttgcgtcgc agcaacaacg tgtttgcagg
    28801 atcccgagct tgcgctaaat tgaagttgtg cactgcacca aacgaatcgg aatcccggaa
    28861 ctttctcacg gaaagactgg tcggaattgt gcagaatcaa ggaactaacc cgggcgcccg
    28921 gctactattg cgccacaaca ttttttgata aggcctccgc ccagaaggcg gttgccgaaa
    28981 cgagggccac acccggcccc gacggagaac gcatcatgaa aacgcccaaa ctgctgccct
    29041 ggtatgcccg caaagcgggc gtctcgatcg agcgcgccga aaccctgtgg cgcaaggccg
    29101 tgcgcgaagc caccgccgaa accggctggg tcggcaatgc cgaatactgg ggtgcggcca
    29161 tggatcactt cgtgcgtctg ctcgaagaag agcgcagcac gttgtgcgcg ccgcgcgtgc
    29221 agtcctacgt ccgcacccag aaccgcatgt ggcgcctgcc gctgatcgcc atggaagacc
    29281 tgttctcggc cttccacgca agctggcagc gccagcacgg cgccgaccac cgccgcgccg
    29341 cgtaagcggc accccgcccc gaacccctcg cgccgcgcca gcggcgcgac aacgcttgct
    29401 tgtctccctc cctccttcaa tggagattcg gcgctccgcc cacgcggagc gccatttttt
    29461 tgcccgcagc aagcctgttt gcgcgccgcg cgggcttcgc ggcgagccct gccttggccc
    29521 ggcctgccgc ggcaggcatc gggagcgccc ggtgtcgcgc ttcacggtat actggcgtgt
    29581 tttcaacgtc atccagcccg cgcgcccgcc atgcaggaaa aataccagcc cgccgccctc
    29641 gagacggccg cccagcagca ctgggactcc accgacgcct tccgcgtgac cgaggatgcc
    29701 agccgtccga agtactactg cctgtcgatg ttcccctacc cctcgggcaa gctgcacatg
    29761 gggcacgtgc gcaactacac catcggcgac gtgctcgcgc gcttccaccg catgaagggc
    29821 tacaacgtgc tgcagcccat gggctgggac gccttcggca tgccggccga gaacgccgcg
    29881 atccagaaca acgtgccgcc ggcgaagtgg acctacgcca acatcgacta catgaaggcg
    29941 cagttgaagc gcctcggctt cgccatcgac tggtcgcgcg agctcgccac ctgcaccccc
    30001 gagtactacc gctgggagca gtggctgttc acccgcctgt acgagaaggg cctgatctac
    30061 aagcgcctcg gcacggtcaa ctgggaccct gtggacgaga ccgtgctcgc caacgagcag
    30121 gtcatcgacg gccgcggctg gcgctcgggc gcgctcgtcg agaagcgcga gatccccatg
    30181 tactacatga agatcaccgc ctacgcggac gaactgctgt ccgcgctcga cgacctgccg
    30241 gactggccgg agcaggtcaa actgatgcag aagaactgga tcggccgctc ggaaggcgtg
    30301 gagatcagct tcccgttcga cgtttccagc atcggtcacg agggcgcgct gaaggtattc
    30361 accacccgtg ccgacaccct gatgggcgcg acctatgtcg cggtggccgc cgagcatccg
    30421 ctcgccaccc aggccgcggc cagcaacccg gcgctcgccg agttcatcga ggaatgcaag
    30481 cgcggcggcg tcgccgaggc cgacatcgcc acgatggaaa agaagggcat gcccaccggc
    30541 ctcaaggtgc gccatccgct ttccgacgag ccgctcgagg tgtgggtcgc caactacgtg
    30601 ctgatgggct atggcgaagg cgcggtgatg gcggtgccgg cgcacgatga gcgcgacttc
    30661 gcctttgcca ccaagtacga gctgccgatc cgcatggtgg tcggcgcgga cgcctacgcc
    30721 gacaccgtcg ccccgtggca ggacgcctac gccgagtacg gccgcctggt gaattccggc
    30781 aagtacgatg gcctggattt ccagggcgcg gtcgatgcga tctccgccga cctcgaagcc
    30841 cgtggcctgg gcgccaagcg cgtgcagttc cgcctgcgcg actggggcat ctcgcgccag
    30901 cgctactggg gctgcccgat cccgatgatc cactgcgccg actgcggcga cgtgccggtc
    30961 ccggacgacc agctcccggt cgtgctgccc gagaacgtcg agatcaccgg ccgcggctcg
    31021 ccgctggcca agatggcctc gttctacgaa tgcagctgcc cgaagtgcgg caagccggcc
    31081 aagcgcgaga ccgacaccat ggacaccttc gtggagtcgt cgtggtactt cctgcgctac
    31141 gcctgcgccg acagcacgac tgcgatggtg gacgcgcgcg tgaactactg ggcgccggtc
    31201 gaccagtaca tcggcggcat cgagcacgcc atcctgcacc tgctgta
//
LOCUS       DAOQBS010000023        31140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_852_length_31140_cov_15.707319, whole genome shotgun sequence.
ACCESSION   DAOQBS010000023 DAOQBS010000000
VERSION     DAOQBS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 31140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31140
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_852_length_31140_cov_15.707319"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1331)
                     /gene="nagZ"
                     /locus_tag="PKN25_04565"
     CDS             complement(186..1331)
                     /gene="nagZ"
                     /locus_tag="PKN25_04565"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNV90204.1"
                     /translation="MNPPIRRPRGPVMIDVAGTALTDEERERLRDPLVGGVILFARNY
                     TGSEQLRALTAEIRGLRDPALIIAVDHEGGRVQRFRTDGFTRLPSMRSLGALWAQDHL
                     VALDAARATGVVLAAELRAHGVDLSFTPVLDLDYGCCRAIGNRAFHRDPQVVAALAQA
                     LCAGMAEAGMGCVGKHFPGHGFVEADSHHDVPVDERDFDTVWNEDIAPYRHRLGRQLA
                     GVMPAHVVYPNADPSPEPQPAGFSPFWLKEVLRDRLGFQWVIFSDDLNMEGARVAGDI
                     VGRAKAAYAAGCDMLLVCNRPDLAAELLDRWAPDLDAGNLARLAAILPDTARPAWLAD
                     PFALELHAPYLRAREHLASIPEDKSAAPTMTAATIGEQRTEVLRKEG"
     gene            complement(1351..1728)
                     /gene="acpS"
                     /locus_tag="PKN25_04570"
     CDS             complement(1351..1728)
                     /gene="acpS"
                     /locus_tag="PKN25_04570"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNV90205.1"
                     /translation="MIHGIGTDLVQLRRMREALERHGERFALRILAASEVEAWRAHRD
                     PARFLAKRFAAKEAFGKALGTGVAVPATLHAVAVGHDARGKPEYRYDERLAAHMRDNA
                     LRAHLSISDEDEHIVAFALIESV"
     gene            complement(1926..2678)
                     /locus_tag="PKN25_04575"
     CDS             complement(1926..2678)
                     /locus_tag="PKN25_04575"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNV90206.1"
                     /translation="MIELGVNIDHVATLRQARRTWEPDPVWAAVEAHLGGADGITIHL
                     REDRRHIGDEDVRRLAELTQVKLNLEMAATDEMVAIACRTKPQMAMLVPEGRHEITTE
                     GGLDVLAQETRLKDVIARLADAGIVTSVFIDAEPEQIEAAARIGARVCEIHTGPYAHA
                     FHAAGRDAEAPAVLAELDKIRTAGEVVRSLGMRFNAGHALNYYNVEPIARLHGIRELH
                     IGHSIVSRAVFVGLRAAVAEMKRLMREAAQGA"
     gene            complement(2678..3436)
                     /gene="recO"
                     /locus_tag="PKN25_04580"
     CDS             complement(2678..3436)
                     /gene="recO"
                     /locus_tag="PKN25_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNV90207.1"
                     /translation="MAAAKQRIEQQPAWVLHTYPWRETSLIVEIFAREHGRVALVAKG
                     ARRPHSQLRGVLLAFQPLWMDWSGGGEVKTLVRAEWQGGQPLLIGRALICGYYLNELL
                     VRLTAREDPHPRLFDAYAEAVRALGRGEAESPILRRFELALLQELGYEAGLANEGDSG
                     EPVRPEGRYLYIIERGPVRLEALEEEGAALDPAALGEQPLLSGQTLLDMAAGDFSRAE
                     TLGQSKQLLRMLINHTLGGQPLQSRRVLKELQEL"
     gene            complement(3452..4381)
                     /gene="era"
                     /locus_tag="PKN25_04585"
     CDS             complement(3452..4381)
                     /gene="era"
                     /locus_tag="PKN25_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNV90208.1"
                     /translation="MSDASQTPLSDQPTGEDGFRAGFVAIVGRPNVGKSTLLNRLIGQ
                     KISIVSSKAQTTRHRVTGILTEPGAQFVFVDTPGFQTHHRNALNRSMNRTVSQVLADV
                     DLVFFVIEAGRFTEDDRKVLAVIPESSKVILVINKVDQLKDKTRLLPFIAQVREARDF
                     TEIVPLSAEKGRNVDELVKAATPLLPVGTPMFEEDEITDRSERFLASEFLREKLFRLL
                     GDELPYGIAVEIEKFEVEGNLRRIYAAVIVDKPGHKSMVIGRGGEKLKRISSEARVEL
                     EKLFDGKVFLEVWVKVKSGWADDERALKSLGYE"
     gene            complement(4378..5049)
                     /gene="rnc"
                     /locus_tag="PKN25_04590"
     CDS             complement(4378..5049)
                     /gene="rnc"
                     /locus_tag="PKN25_04590"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNV90209.1"
                     /translation="MMPDRLQARLGHAFSDVRLLQEALTHRSFGQPNNERFEFLGDAV
                     LDCVVSIALFGRFGELREGELSRVRASLVRQDTLHRLALELELGDCLRLGEGELKSGG
                     SRRPSILADALEALFAAVYLDAGFEAAKGVIDRLYAPLIAEVDPCKPSKDPKTALQEW
                     LQARRIPVPSYTMVEIVGEAHAQEFEVACEIPKLAVRTLGRGPSRRIAEQQSAELALA
                     AVRKK"
     gene            complement(5054..5416)
                     /locus_tag="PKN25_04595"
     CDS             complement(5054..5416)
                     /locus_tag="PKN25_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HNV90210.1"
                     /translation="MKMRSQRGLTLLGVLVTGVIAAFVLVVGFRSVPVINEYLAIKRI
                     LGIVADESDKGASLIEVRRSFDRRRQIDDVSSVGGADLTIDKSGVRTVIDVEYTRKIP
                     LLANVSLSIDLHPSSEPR"
     gene            complement(5430..6218)
                     /gene="lepB"
                     /locus_tag="PKN25_04600"
     CDS             complement(5430..6218)
                     /gene="lepB"
                     /locus_tag="PKN25_04600"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNV90211.1"
                     /translation="MNFALILFLLLIASGALWSVDRFHARKLRPAGTPDPWWVEYGAS
                     FFPVILIVFGLRSFVVEPFKIPSGSMIPTLLVGDFILVNKWTYGIRLPVINRKIVAVN
                     DPQRGDVMVFRYPADPSMDYIKRVVGLPGDRVEYIDKRLTINGEAVPIREDGSYLHPD
                     RLYYSPKYVERLGEVEHSVLIERDAPAYVPQVLNFPGREHCTYVHAGVRCTVPAGHYF
                     VLGDNRDASSDSRVWGFVPDQNIVGRAFFIWFNFGDMKRIGGFD"
     gene            complement(6259..8055)
                     /gene="lepA"
                     /locus_tag="PKN25_04605"
     CDS             complement(6259..8055)
                     /gene="lepA"
                     /locus_tag="PKN25_04605"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNV90212.1"
                     /translation="MKHIRNFSIIAHIDHGKSTLADRLIHFCGGLSEREMEEQVLDSM
                     DIERERGITIKAQTAALHYKAKDGQVYNLNLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DASQGVEAQTVANCYTALDLDVEVVPVLNKIDLPAADPENARGEIEDVIGVDASEAIL
                     CSAKTGLGVEEVLEAIVARVPPPQGEPDGPLKALIIDSWFDNYVGVVMLVRVVDGELK
                     PKDRILLMSNGLQYPCDQVGVFTPRSVGRDALRAGEVGFVIAGIKEIEAAKVGDTITL
                     ANKPASAPLPGFKEIKPQVFAGLYPVESSEYDQLRDSLEKLRLNDSSLQFEPEVSQAL
                     GFGFRCGFLGLLHMDIVQERLEREFDMDLITTAPTVVYEVVLNDNSIIRIENPAKLPE
                     PGKYQEIREPIITATIFLPQDYVGPVITLCNQKRGNQIDMRYHGRQVQLIYEMPMNEV
                     VMDFFDRLKSVSRGYASLDYDFKEYRTADLVKLDLMVGGEKVDALSVIVHRASAQYRG
                     RELAAKLRGLIPRQMFDVPVQAAIGAHIIARETIKALRKNVLAKCYGGDITRKKKLLE
                     KQKEGKKRMKQVGNVEIPQEAFLAVLRTDEGK"
     gene            complement(8134..8394)
                     /locus_tag="PKN25_04610"
     CDS             complement(8134..8394)
                     /locus_tag="PKN25_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNV90213.1"
                     /translation="MERRFTVMSREWCHLCHVLVDALRPLAEAAGWGIEVIDVDQHPE
                     LEARWGEWVPVLLHGEHELCHYHLDEAAVRAYLGGFPLESRA"
     gene            complement(8406..9869)
                     /locus_tag="PKN25_04615"
     CDS             complement(8406..9869)
                     /locus_tag="PKN25_04615"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNV90214.1"
                     /translation="MNPEVRMQTSAARALAVVLCLLLMLCAPRGFAAGTAERAMLPDF
                     TRLVATQGAAVVNISATQVAAQPQKQPFRLPELDESDPMFEFFRKFIPRMPEYPGAEP
                     DDKSLGSGFIISADGFILTNAHVVEAAESIVVRLADKREFDATVIGADARSDVALIRI
                     EAKDLPHVVLGDPEALAVGEWVLAIGSPFGFEQSVTAGIVSAKGRSLPDENFVPFIQT
                     DVAINPGNSGGPLFNLRGEVIGINSQIYSRTGGFMGLSFAIPIDVAMDVQQQLREKGR
                     VERGRIGVSIQEITRDLADSFGLPRPAGALVSSVEAGGPAALGGVVQGDVIVRFNQRN
                     VENSADLPRIVAAARPGSKVEVEIYRDGAPRSLSLTLGEWRDPEEEVEPVAVGLATGA
                     TNRLGLELVAPTAQQRRERGLAHGLLVQRAEKSAARAQIVPGDLVLAIVVEGRQVRLD
                     RIEDFERVVAALKPGQQVTLLVGRGESASYVSLRADK"
     gene            complement(9866..10336)
                     /locus_tag="PKN25_04620"
     CDS             complement(9866..10336)
                     /locus_tag="PKN25_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNV90215.1"
                     /translation="MMEARARILRIENGQAWVKLSGKQGGCGRCDEPGGCGSVQISHV
                     FGMPRDEFSIPADARFAAGDAVVIRIPDGAPLRAALVSYGLGTLLLLVGAAFGSLLGG
                     VQHGDAWGLAGGVAGLALAWVVNRMLSRSRNWRNSLSMELSVEENCSRRLRVLQ"
     gene            complement(10333..11301)
                     /locus_tag="PKN25_04625"
     CDS             complement(10333..11301)
                     /locus_tag="PKN25_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HNV90216.1"
                     /translation="MRWFPAMLGLCALLAQGPASAEHPTDPLSWLGRISTAGQRLNYV
                     GTFVYQSGSHVETSRIAHRVDASGEYERLEVLDGSPREIIRRGAEVRCVLPDERTVII
                     DEAQGRRAFPARLPASPTGLAENYRVRKGELGRVAGLEAQALVLEPKDELRYGHVLWA
                     DVQSGLLLKSRTLDQHGAVIEQFSFSELRIGGEVENSLLESRFKTDEGWRMVNARGVS
                     MSVEAAGWVLEPAVPGYALTSAMKRPLGRDRGEAVHMVFSDGLAAISVFIEPIGEGSR
                     AGLGPLSSGAINIFKRAIDGHLVTALGEAPEAAVRRIGEGIRPEAR"
     gene            complement(11298..11837)
                     /locus_tag="PKN25_04630"
     CDS             complement(11298..11837)
                     /locus_tag="PKN25_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HNV90217.1"
                     /translation="MKDKVSALFDGDLDDQGMRAVFDGMRKDASLRKEWDTYCLIGDT
                     LRGDSAGSSDFVSRVMVSLDDEPTLLAPGVPAAGLAQRGLVHRLMPLAASVMGVAAVG
                     LVAASLYRQDAPAPQMAVAASPIQFVGSSNVRPVAAPAADPMREYLFAHQGLSRGGPM
                     PAGVQYVRTVSDTREGGAR"
     gene            complement(11846..12445)
                     /gene="rpoE"
                     /locus_tag="PKN25_04635"
     CDS             complement(11846..12445)
                     /gene="rpoE"
                     /locus_tag="PKN25_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HNV90218.1"
                     /translation="MSDRELDQQLVERVQRGDKQAFGLLVSKYQRKLNRLLSRLIRDS
                     AEVEDVAQETFIKAYRALGSFRGDSAFYTWLYRIGINTAKNYLVSQGRRAPTSTSFDS
                     EEAETFEEGDQLRDINTPERLLMTRQIGETVEDAMAALPEELRTAIMLRELEGLSYEE
                     IAGIMECPIGTVRSRIFRAREAIAERLRPLLDTAPDKRW"
     gene            12663..14261
                     /gene="nadB"
                     /locus_tag="PKN25_04640"
     CDS             12663..14261
                     /gene="nadB"
                     /locus_tag="PKN25_04640"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNV90219.1"
                     /translation="MLKQYDVLILGSGTAGQSMALRLADRLRVALVTKRELADSASNW
                     AQGGIAAVLDNTDSIEAHIQDTFVAGAGLCNPTSTRFVVENGKRAIEWLIDRGVPFTR
                     EADSQLGYHLTREGGHSHRRIIHAADATGAAVQATLGDHVRRHPNIDIYEHHIAVDLI
                     TADKLGQSGAGCLGAYVLDIHGERVLTFAARNTVLATGGTGKVYLYTTNPDVATGDGV
                     AMAWRAGCRVGNMEFIQFHPTCLYHPQAKSFLISEAVRGEGGLLKLPDGTRFMPEHDP
                     REELAPRDIVARAIDFEMKKRGIDCVYLDISHKPADWLRGHFPNIHARCLELGIDITK
                     EPIPVVPAAHYSCGGIMTDLAARTGVPGLYAVGESACTGLHGANRLASNSLLECLVFG
                     EAAAEDILSKAQEPAPVLPAWDESRVTDADEEVVISHNWAELRRFMWDYVGIVRTNKR
                     LRRAQHRIRLLSREIHEFYSNFRVSNDLIELRNLVTTADLIVRCALKRKESRGLHHSR
                     DYPETLPRARDTVLRPPARTPRKR"
     gene            complement(14310..14807)
                     /locus_tag="PKN25_04645"
     CDS             complement(14310..14807)
                     /locus_tag="PKN25_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90220.1"
                     /translation="MQFPVELKLRGCSLVKASVWVVHAAAALALFHVPVFTDWEAGDV
                     DRAIAACAWALIFLSLFRGLRAQARLDGCTLWLERDGALELLQEPDGEGGLYRVRERS
                     QVVLPMAAWFTLVPAQISAPGQGAPVARTLFLVPGNLSAGSFRLLRVWLRHRSGRVGP
                     DAAAR"
     gene            complement(14830..16065)
                     /gene="fabF"
                     /locus_tag="PKN25_04650"
     CDS             complement(14830..16065)
                     /gene="fabF"
                     /locus_tag="PKN25_04650"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNV90221.1"
                     /translation="MSRRRVVVTGLGVISPVGNTVTEAWSNLIAGKSGIGPITRFDAS
                     NFAARIAGEVKDFDVSAYLSPKEARRMDVFIHYGMAAGIQAIRDSGIEVTEANADRIG
                     INIGSGIGGLPMIEDTHNDFLGGGPRKISPFFIPGTIINMISGNLSIMFGMKGPNLAV
                     VTACTTGLHAIGMGARMIGYGDADVMVCGGAESTVTNLAIGGFGSAKALSTRNDDPAT
                     ASRPWDKGRDGFVLGEGAGVMVLEEYEHARRRGAKIYAEVAGFGMSGDAYHMTAPDTD
                     GPRRSMVNAMKDAGVNVDQVQYLNAHGTSTPLGDKNETDAIKLAFGVEAAKQLVVNST
                     KSMTGHLLGGAGGLESVVTALAVYHQISPPTINIFEQDPECDLDYCANVAREMKIDVA
                     LKNNFGFGGTNGTLVFRRV"
     gene            complement(16165..16404)
                     /gene="acpP"
                     /locus_tag="PKN25_04655"
     CDS             complement(16165..16404)
                     /gene="acpP"
                     /locus_tag="PKN25_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNV90222.1"
                     /translation="MENIEQRVKKIVAEQLGVNESEIKTESSFVDDLGADSLDTVELV
                     MALEEEFECEIPDEEAEKITTVQQAIDYVNAHLKK"
     gene            complement(16493..17242)
                     /gene="fabG"
                     /locus_tag="PKN25_04660"
     CDS             complement(16493..17242)
                     /gene="fabG"
                     /locus_tag="PKN25_04660"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNV90223.1"
                     /translation="MSFSLEGQVALVTGASRGIGRAVALELGRLGATVVGTATSESGA
                     ADIGKALADAGIQGAGMALDVTDAAACEALVGEVEKRFGAVGILVNNAGITRDNLAMR
                     MKDEEWDAVVDTNLRAVFRMSKLVMRGMMKARHGRIINITSVVASSGNPGQANYAAAK
                     AGVAGMTRALARELGSRNITVNCVAPGFIDTDMTRALPEAARDALLGNIALGRLGRPE
                     EIAGAVAFLASPAAAYVTGTTLHVNGGMYMS"
     gene            complement(17246..18175)
                     /gene="fabD"
                     /locus_tag="PKN25_04665"
     CDS             complement(17246..18175)
                     /gene="fabD"
                     /locus_tag="PKN25_04665"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNV90224.1"
                     /translation="MAFALVFPGQGSQSVGMMAAYGESAVIRDTFAEASEALGEDLWA
                     MVCDGPAERLALTVNTQPLMLTAGVAAWRAWRGAGGPKPALVAGHSLGEYSALVAAGA
                     LAFKDAVPLVRFRAQAMQEAVPAGEGAMAAVMGLEPDAVHAACAEAAQGQVVEAANLN
                     APGQIVIAGAKAAVERASEIAKAKGAKRAVLLPVSAPFHCALMRPAAERLAERLAGVA
                     IVKPEIDVINNVDVAVYDDPEKIRDALVRQAYSPVRWIELVQAMGARGMSHVIECGPG
                     KVLAGMTKRIAKEVEGGSAHDAASLEQAIAAVR"
     gene            complement(18221..19186)
                     /locus_tag="PKN25_04670"
     CDS             complement(18221..19186)
                     /locus_tag="PKN25_04670"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNV90225.1"
                     /translation="MIYARIAGTGSYLPGEPVSNDDLVKRGIDTSDEWIVSRSGIRSR
                     HLAAADTGASDLARVAAERAIEAAGCEAEDIDLIIVATSTPDYIFPSTAALVQSKLGI
                     RNGGAAFDVQAVCSGFIYALSVAEKFIRSGSNKRALVIGAEVFSRILDWSDRGTCVLF
                     GDGAGAVVLEASERPGILASALHADGSHHPILCVPGAVASGQVIGDPFLRMDGQAVFK
                     FAVKVLGDVANEVLDAAGVQASSVDWLIPHQANIRIIQATAKRLGVPMERVVTTLDHH
                     GNTSAASIPLALDEAVRAGRLRPGHRIVVEGVGGGFTWGAALIDF"
     gene            complement(19183..20199)
                     /gene="plsX"
                     /locus_tag="PKN25_04675"
     CDS             complement(19183..20199)
                     /gene="plsX"
                     /locus_tag="PKN25_04675"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNV90226.1"
                     /translation="MGVTIAIDCMGGDHGPVVTVPAALEFLRSHPQASAILVGREEAI
                     APLLGNATTEFGARIRVRAASEVVAMDDPPAIAMRNKKDSSMRVAVDLVKAGEASAAV
                     SAGNTGALMAISRFVLKTLPGIDRPAIATVLPSMKGHTYVLDLGANVDCSAEHLLQFG
                     VMGAMLVAAIEHKDRPTVGLLNIGEEVIKGNDVVKEAGELLRNSGLNFQGNVEGNDIY
                     EGTVDVVVCDGFVGNVALKTSEGLAQMVGAFLKQEFKRTLFTKLLAVAAMPVLKRFKK
                     RVDHRLYNGAPLLGLRGVVLKSHGSADVLAFGNALSRAAEAASNRLIERISERMASMN
                     EAKA"
     gene            complement(20295..20474)
                     /gene="rpmF"
                     /locus_tag="PKN25_04680"
     CDS             complement(20295..20474)
                     /gene="rpmF"
                     /locus_tag="PKN25_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNV90227.1"
                     /translation="MAVQQNKKSPSKRGMHRAHDFLTGPALAVEPTTGETHLRHHVSP
                     SGFYRGKKVAKGKGE"
     gene            complement(20504..21028)
                     /locus_tag="PKN25_04685"
     CDS             complement(20504..21028)
                     /locus_tag="PKN25_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HNV90228.1"
                     /translation="MSQHGDPSRIISDPFRFAAEARTLEGVTAVAEMLRLSDVLASSG
                     GQVSWRIEGSMVEGVEAREPRLHLMIEGELQLCCQRCLGGMAWPVQVDSVLQPVRSGQ
                     PIPEEELEDDEIDAIELDGELDVIALVEDEILLALPIAPRHQVCDAPRPDGGASKESP
                     FAALASLRGSHSAK"
     gene            21170..21745
                     /locus_tag="PKN25_04690"
     CDS             21170..21745
                     /locus_tag="PKN25_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNV90229.1"
                     /translation="MKIVLASTSAYRRMLLERLQLPFETDRPETDESPLPGEAPAATA
                     ERLALEKARAVAARWPGALIIGSDQVAHLGNEVFGKPGTEARAIAQLQRMRGQTVVFH
                     TALALLDTRSGHAQVEGVPTHVRFRELSDDEIRRYVERERPLDCAGSAKSEGLGIALL
                     EAMRGDDPTALIGLPLIALARMLRTAGVHLP"
     gene            21742..22470
                     /locus_tag="PKN25_04695"
     CDS             21742..22470
                     /locus_tag="PKN25_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNV90230.1"
                     /translation="MSGDAMPRGNLYLVPVALGEHTPWAHFLPAQAQAIAAGLRHFVV
                     ETARAARAHLKQLDYPHPLRDTDIRELPAEAADAALDALLAPALAGTDLGLLSDAGCP
                     AVADPGARLVARAHALGIRVVPLVGPSSILLGLMGSGLNGQSFAFHGYLPVAEGERDG
                     ALRTLEDESRRLGRTQIFIETPYRNERMLDALLRTLKPETRLCVACELNTADEFLLTR
                     SVREWKKAARPALARRPALFLLLA"
     gene            complement(22486..23142)
                     /locus_tag="PKN25_04700"
     CDS             complement(22486..23142)
                     /locus_tag="PKN25_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNV90231.1"
                     /translation="MAERFDLIVFDWDGTLMDSAAAIVRAMQAAARDLDLPVPSEERA
                     RYVIGLGLGDALRHAVPDLEESDYPRMVERYRHHYLSSDHELSLFEGVDALIDALAGR
                     GHLLAVATGKSRVGLNRALGHTGLGRYFHATRCADECFSKPHPAMLEALMDELGVAPE
                     RTLMIGDTTHDLQMAKNARTAGLAVGFGAHPVEVLVAESPLACVRTPGELAGWLLANA
                     "
     gene            complement(23135..24094)
                     /locus_tag="PKN25_04705"
     CDS             complement(23135..24094)
                     /locus_tag="PKN25_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNV90232.1"
                     /translation="MTTIEGKAIAVRHERIDEAAAGQRIDNYLLRIAKGVPKSHVYRI
                     LRSGEVRVNGRRVQQTYRLQEGDEVRIPPIRIAEPTHAAPAPAGKPLPVVYEDEALLV
                     IDKPSGKAVHGGSGVSYGVIEQLRVQRPELRMLELAHRLDRETSGLLIVAKKRSALTA
                     LHDMMREGSVEKRYLTLVPGRWSNPLQHVKVPLHKYLTAEGERRVRVADEGKAAHSIV
                     RLVRRWRDYSLLEVELKTGRTHQIRVHLAHLGFALCGDDKYGDFALNKRLEVEGLRRM
                     FLHAAKLRLAHPLTGAPLAFDSPLPADLQTFLDRLDAAEKKHG"
     gene            24546..27722
                     /locus_tag="PKN25_04710"
     CDS             24546..27722
                     /locus_tag="PKN25_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNV90233.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQKLIDLDIESAAKEERKSNIYKA
                     VITRIEPSLEAAFVDYGADRHGFLPFKEISRSYFQPGADAKASIKEALKEGQELIVQV
                     EKDERGNKGAALTTYISLAGRYLVLMPNNPRGGGVSRRVEGDERAELRENMDQLDVPQ
                     GMSLIARTAAIGRSAEELQWDLNYLLQLWTAIEGAAQGQSGAFLIYQEGSLVIRAIRD
                     YFQPDIGEILIDTDDIFEQATQFMAHVMPGNVARVKRYSDDVPLFSRFQIEHQIESAY
                     SRQVNLPSGGAVVIDHTEALVSIDVNSGRATKGSDIEETAFRTNCEAADEIARQLRLR
                     DLGGLIVIDFIDMESAKNQREVENRLRDALRHDRARVQTGKISRFGLLELSRQRLRPA
                     LAETSYITCPRCNGTGHIRSTESSALHIVRILEEEAMKDNTGAVHLQVPVDVATFLLN
                     EKRVDIARIEMRHKVQLIIVPNRHLETPAHEIIRLRHDQLNTEDIALASYQMVQKPVE
                     EDTRLPSKANERPARPEAAVKGIAPAQPAPMAAPEPAPASAPAPAAPEPKGLIARIMG
                     WFSAGKGAAEPVPAPVVEETPKREARPRGERSGEGRRNGGSNGGRGRRGERDGEAQPS
                     TRGSRAERGESTERPQRVAAERAERPERAERPERGERAERSEGGRRGRGQRGERAQEG
                     AEQRAAQTEDDLLKAATATAMVAAATTADSGTTETVASTEGGEEEGTPSEKRRRRGRG
                     RGRRSGEPQGIDGAAEAGNEVETGATAPEGELSGIAASAVEQGTVESAHAASPVTAGQ
                     DANVGAAATAVVQTAPADAQIAELPIVGGTAEDVTPVALAAPQVPLAPVPVGAALEVV
                     AETAPAAEVPVDKALAAEAVLAEQGAAAEELAAGSAATVEAVSAAAPRAETAAMIDSA
                     SAEEAPPAVASAAGEEAVIGTEPVAVAEPAPTSSAGVPPEVAAAAPEAVPAPLADSTE
                     VAEPSVPAAAESTVVRAMEAGRASHAPSAPIAVPSGDSGLIRIETDPAKRVQFGTGAP
                     EQPARLGRQPRPAAVIAEEPLQQVETRK"
     gene            complement(27876..28349)
                     /locus_tag="PKN25_04715"
     CDS             complement(27876..28349)
                     /locus_tag="PKN25_04715"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNV90234.1"
                     /translation="MKRILFVCTGNICRSPTAEAVARHFVETGGLGAQVLLDSAGTQG
                     YHAGEAPDPRTQKAAKLRGYDLSALRARKLELRDFQEFDLLLAMDRGHLEHMQRHCPE
                     VYRPKLQLFLPFAGAQAHGEEVPDPYYGGPKGFDLVLDMCEDAARGIVQRVRGEG"
     gene            complement(28374..30461)
                     /gene="uvrB"
                     /locus_tag="PKN25_04720"
     CDS             complement(28374..30461)
                     /gene="uvrB"
                     /locus_tag="PKN25_04720"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNV90235.1"
                     /translation="MTTQGNSAGNDAPQQGGTVLGFEGSPFRLHQPFPPAGDQPEAIR
                     LLCEGVEDGLMYQTLLGVTGSGKTYTMANVIARMGRPALVLAPNKTLAAQLYAEFKEF
                     LPENAVEYFVSYYDYYQPEAYVPSRDLFIEKDSSINEHIEQMRLSATKSLMERRDVVI
                     VATVSCIYGIGDPVDYHAMILHLREGERMAHRDLIQRLVAMQYTRADIDFRRGTFRVR
                     GDVIDVFPAENAELAVRIEMFDDEIEHLTLFDPLTGHLRHKLARFTVYPSSHYVTPRA
                     TVLQAIEAIKEELKERVAFFQREGKLVEAQRIEQRTRFDLEMLNEMGFCKGIENYSRH
                     LSGRGPGEPPPTLIDYLPQDALLFIDESHVAIGQVGGMYKGDRSRKENLVGYGFRLPS
                     ALDNRPLKFDEFERLMPQTVFVSATPAKYEEEHQGQVVEQVVRPTGLIDPMVEVRPAM
                     TQVDDLLSEIKKRLEVNERVLVTVLTKRMAEDLTDYLADNGIRVRYLHSDIDTVERVE
                     IIRDLRLGEFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARH
                     LHGTAILYADRVTDSMKAAIGETERRRHKQIAFNEANGIVPKSVTKRIKDIIDGVYDA
                     DGAKRDVARVAEKGGDYAAMDEKALARAIKRLEKEMQEHARNLEFEQAAAARDELFKL
                     RERAFGADQHGSV"
     gene            30613..>31140
                     /locus_tag="PKN25_04725"
     CDS             30613..>31140
                     /locus_tag="PKN25_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV90236.1"
                     /translation="MSASIFAAVEMAPRDPILGLNEAFAADTRAEKVNLGVGVYYDDN
                     GKIPLLAAVRTAEKARLEAMPPRGYQPIEGPAAYNNAVQGLLFGKDSQLATSGQTITV
                     EALGGTGALKVGADYLKRLVPGATVYISDPSWENHRALFESAGFPVENYPYYDAATRG
                     VNFAGMKAKLDSLQPG"
BASE COUNT         5073 a        10863 c        10340 g         4864 t
ORIGIN      
        1 gcaccttgtc gtcggcgccg atcatgcgat agacgcccgg ctcctcggtc agggtgcgca
       61 ggaagggctt ggggtcgaag gcgggggcgg gcgtcgggtc ggtcatggga gcaggatgtt
      121 tgagcaacgc cgctgatcgc gacttgcgtc gctcctacag gggagttcgc taccgctgcg
      181 cggaattatc cttccttgcg caggacttcg gtacgttgct caccgatggt ggcggcggtc
      241 atggttggcg cggcgctctt gtcctcggga atggacgcga ggtgctcgcg ggcccggagg
      301 tagggggcgt gcagttcgag tgcgaagggg tcggcaagcc aggctggcct ggccgtgtcc
      361 ggcaagatcg cggcgagtcg ggccaggttg ccggcgtcca ggtccggcgc ccagcgatcc
      421 agcagctcgg ccgcgaggtc aggtcgattg cacaccagca gcatgtcgca gccggccgcg
      481 taggctgctt tcgcacggcc gacgatgtca ccggcgacgc gggcgccttc catgttgaga
      541 tcgtcgctga agatcaccca ctggaagccg aggcggtcgc gcagcacctc cttcagccag
      601 aacggcgaga agcctgcggg ctgcggttcg gggctggggt cggcgttggg gtagacgacg
      661 tgggcgggca tgacgccggc gagctggcgg ccgagacgat ggcggtaggg ggcgatgtcc
      721 tcgttccaaa ccgtgtcgaa gtcgcgctcg tccaccggca cgtcgtggtg cgagtcggcc
      781 tcgacgaagc cgtggccggg gaagtgcttg cccacgcagc ccatgcccgc ctccgccatg
      841 ccggcgcaca gcgcctgcgc gagcgccgcg accacctgcg gatcgcgatg gaaggcgcgg
      901 ttgccgatcg cgcggcagca gccgtagtcg agatccagca ccggggtgaa gctcaggtcg
      961 accccgtgcg cgcgcagctc ggcggcgagc acgacgccgg tggcgcgcgc cgcgtcgagc
     1021 gccaccagat ggtcctgcgc ccacaaggcg ccgaggctgc gcatcgacgg caggcgggtg
     1081 aagccgtcgg tgcggaagcg ctgcaccctg ccgccttcgt ggtcgaccgc gatgatcagc
     1141 gccgggtcgc gcagcccgcg gatctcggcg gtgagcgcgc gcagctgctc ggagccggtg
     1201 tagttgcgcg cgaacaggat caccccgccg accagagggt cgcgcaggcg ttcgcgctcc
     1261 tcgtcggtga gcgcggtgcc ggcgacatcg atcatgacgg ggccgcgtgg gcggcggatg
     1321 ggtggattca tgttctggag gctccggggc tcacacgctt tcgatcaggg cgaacgcgac
     1381 gatgtgctcg tcctcgtcgc tgatgctgag gtgggcgcgc agcgcgttgt cccgcatgtg
     1441 cgcggcgagg cgctcgtcat agcggtattc gggcttgccg cgcgcgtcgt ggccgaccgc
     1501 aaccgcgtgc agcgtggccg gcaccgcgac gccggtgccg agcgccttgc cgaaggcttc
     1561 cttggcggcg aagcgcttgg cgaggaagcg cgccgggtcg cgatgggcgc gccaggcctc
     1621 gacctcgctc gcggccagga tgcgcagggc gaaacgctcg ccgtggcgct cgagcgcctc
     1681 gcgcatccga cgcagttgca cgagatcggt gccgatgccg tggatcatgc ttgcacgctc
     1741 gcacacaggc tcgcgcgcgc ccctgtcgga gcggcgcaag ccgcgaatgc ggcgcccgga
     1801 tccactccac cgcccggatt gggtgcagca ttcgcggctt gcgccgctcc tgcaggggga
     1861 cgccggcgcg ctgcgctgtc ggcgggtgca agggggcagg ggcgcggcac gcgcatcgcg
     1921 cttcctcagg cgccctgcgc cgcttcgcgc atcagccgct tcatctcggc gaccgccgcg
     1981 cgcaggccca cgaacaccgc acggctgacg atcgaatgcc cgatgtgcag ctcgcggatg
     2041 ccgtgcaggc gggcgatcgg ctcgacgttg tagtagttga gcgcgtggcc ggcgttgaag
     2101 cgcatgccca ggctgcgcac cacttcaccc gccgtgcgga tcttgtcgag ctcggcgagc
     2161 accgccggcg cctcggcgtc gcgtccggcg gcgtggaagg cgtgggcgta ggggccggtg
     2221 tggatctcgc acacgcgcgc gccgatgcgc gcggcggcct cgatctgctc gggctcggcg
     2281 tcgatgaaca cgctggtgac gattccggcg tcggcgaggc gggcgatcac gtccttcagg
     2341 cgcgtctcct gcgcgagcac gtcgagccca ccctcggtgg tgatctcgtg gcggccctcg
     2401 ggcaccagca tcgccatctg cggcttcgtg cggcaggcga tggcgaccat ctcgtcggtg
     2461 gccgccatct cgaggttgag cttgacctgg gtgagctcgg cgagccggcg cacgtcctcg
     2521 tcgccgatgt ggcggcggtc ctcgcgcagg tggatggtga tgccgtcggc accaccgagg
     2581 tgggcttcca cggcggccca caccggatcg ggttcccagg tgcggcgggc ctggcgcagc
     2641 gtggcgacgt ggtcgatgtt gacgccgagt tcgatcatca gagctcctgc aattccttga
     2701 gcacccggcg cgactgcagc ggctggccgc ccagggtgtg gttgatcagc atgcgcagca
     2761 gctgcttgga ctggcccagc gtctccgcac gcgagaagtc gccggccgcc atgtcgagca
     2821 gggtctgacc ggagagcagc ggctgctcgc cgagcgcggc agggtcgagg gcagcaccct
     2881 cttcctccag cgcctccagc ctcaccggac cgcgctcgat tatataaagg tagcgcccct
     2941 cgggccgcac cggctcgccg ctgtcgccct cgttcgcgag cccggcctcg tagccgagct
     3001 cctgcagcag ggcgagctcg aagcgacgca ggatgggcga ctccgcctcg ccgcggccga
     3061 gcgcacgcac cgcctcggcg taggcgtcga agaggcgcgg gtggggatct tcacgtgcgg
     3121 tcaggcgcac cagcagctcg ttgaggtagt agccgcagat cagcgcgcgc ccgatcagca
     3181 gcggctgccc gccctgccat tcggcacgca ccagggtctt gacctcgcca ccgccagacc
     3241 aatccatcca cagcggctgg aaggcgagca gcacgccgcg caactgcgag tgcgggcggc
     3301 gcgcaccctt tgcgaccagg gcgacgcggc catgctcgcg cgcgaagatc tcgacgatca
     3361 ggctggtctc gcgccacggg taggtatgca gcacccaggc cggctgctgc tcgatgcgct
     3421 gcttggctgc cgccatgacc cggggtccgg atcactcgta gccgaggctc ttcagcgcgc
     3481 gctcgtcgtc ggcccagccg ctcttgacct tgacccagac ctcgaggaag accttgccgt
     3541 cgaagagctt ctcgagttcg acgcgggcct cgctggagat gcgcttgagc ttctcgccac
     3601 cgcggccgat gaccatggac ttgtggccgg gcttgtcgac gatcaccgcg gcgtagatgc
     3661 ggcgcaggtt gccctcgacc tcgaatttct cgatctcgac cgcgatcccg tagggcagtt
     3721 cgtcgccgag caggcggaag agcttctcgc gcaggaattc ggaggcgagg aagcgctcgc
     3781 tgcggtcggt gatctcgtct tcctcgaaca tcggcgtacc gaccggcagc aggggcgtgg
     3841 cggccttgac cagctcgtcg acgttgcgtc ccttctcggc cgacagcggc acgatctcgg
     3901 tgaagtcgcg cgcctcgcgc acctgggcga tgaagggcag caggcgggtc ttgtccttca
     3961 actggtcgac cttgttgatg accaggatca ccttcgagga ctcggggatc accgccagca
     4021 ccttgcggtc gtcctcggtg aagcgcccgg cttcgatgac gaagaacacc agatcgacgt
     4081 cggccagcac ctgcgacacg gtgcggttca tcgaacggtt gagcgcgttg cggtgatggg
     4141 tctggaagcc gggggtgtcc acgaacacga actgcgcgcc cggctcggtg aggatgccgg
     4201 tgacgcggtg tcgggtggtc tgcgccttgc tggagacgat gctgatcttc tgcccgatca
     4261 ggcggttgag cagggtcgac ttgccgacgt tggggcgacc gacgatggcg acgaagccgg
     4321 cgcgaaagcc gtcctcgccg gtgggctggt cgctgagggg agtctgggac gcgtcgctca
     4381 tttctttctc accgcggcga gcgcgagctc ggccgactgt tgttcggcga tgcgccggct
     4441 cgggccgcgg cccagcgtgc gtaccgcgag tttggggatt tcgcaggcga cctcgaactc
     4501 ctgcgcgtgg gcttcgccga cgatctcgac catggtgtag ctcggcaccg gaatgcggcg
     4561 cgcctgcagc cattcctgca gcgcggtctt ggggtccttg ctcggcttgc acggatcgac
     4621 ttcggcgatc agcggtgcgt agaggcggtc gatgaccccc ttcgccgctt cgaagccggc
     4681 gtccagatag accgcggcga acagcgcttc gagcgcatcg gcgaggatgg acgggcgccg
     4741 gctgccgccg gacttgagct cgccctcgcc caggcgcagg cagtcgccga gctcgagctc
     4801 gagcgcgagg cggtgcagcg tgtcctggcg caccagcgag gcgcgcaccc gcgacagctc
     4861 tccctcgcgc agctcgccga agcggccaaa cagcgcgatc gacaccacgc agtcgaggac
     4921 ggcgtcgccg aggaactcga aacgctcgtt gttgggctgc ccgaagctgc gatgggtcag
     4981 cgcctcctgc agcaggcgga catcggagaa cgcgtggccc aggcgcgcct gaagtcgatc
     5041 cggcatcatc ggctcagcgc ggctcgctgc tcggatgcag gtcgatcgac aggctgacgt
     5101 tggcgagcag gggaatcttg cgggtgtact cgacgtcgat gacggtgcgg acacccgact
     5161 tgtcgatcgt cagatccgcg ccaccgacgc tgctcacgtc gtcgatctgc cggcgccgat
     5221 cgaagctgcg ccgcacctcg atcaggctcg cccccttgtc ggactcgtcg gcgacgattc
     5281 cgaggatgcg cttgatggcg agatactcgt tgatcaccgg aacgctgcgg aacccgacga
     5341 cgagcacgaa ggccgcgatc acgccggtca ccagcacgcc cagaagcgtg agcccgcgtt
     5401 gggaacgcat cttcatctcc acccccattt cagtcgaagc cgccgatccg cttcatgtcg
     5461 ccgaagttga accagatgaa gaaggcacgc ccgacgatgt tctggtccgg cacgaagccc
     5521 cacacgcggc tgtcgctgct ggcgtcgcga ttgtcgccga gcacgaagta gtgcccggcc
     5581 ggcaccgtgc agcgcacgcc cgcatgcacg taggtgcaat gctcgcgccc ggggaagttg
     5641 agcacctgcg gcacgtaggc cggcgcgtcg cgctcgatca gcacagagtg ctcgacctcg
     5701 ccgagcctct cgacgtattt cggcgagtag tacagccgat ccggatgcag gtagctgccg
     5761 tcctcacgga tcggcaccgc ctcgccgttg atggtgaggc gcttgtcgat gtattcgacg
     5821 cggtcgccgg gcaggccgac cacgcgcttg atgtagtcca tcgacggatc ggccggatag
     5881 cggaagacca tgacgtcgcc gcgctgcgga tcgttcacgg cgacgatctt gcggttgatc
     5941 accggcaggc ggatcccgta ggtccacttg ttgaccagga tgaagtcgcc caccagcaag
     6001 gtcgggatca tcgaaccgga agggatcttg aaaggctcga cgacgaagga gcgcaggccg
     6061 aacacgatca ggatcaccgg gaagaagctc gcgccgtact ccacccacca cgggtcgggc
     6121 gtgcccgcgg gacgcagctt gcgcgcgtga aagcggtcga cggaccacag cgcgccggag
     6181 gcgatcagca acaggaacag gatcagggca aagttcatgc ggtttccgtc ttaggccggc
     6241 ccccagggcc ggcgtcgatc acttgccttc gtcggtgcgc agcacggcga ggaaggcttc
     6301 ctgcgggatc tcgacgttgc cgacctgctt catgcgcttc ttgccttcct tctgcttctc
     6361 gagcagcttc ttcttgcgcg tgatgtcgcc gccgtagcac ttggcgagca cgttcttgcg
     6421 cagggccttg atggtctcgc gcgcgatgat gtgcgcaccg atcgccgcct gcaccggcac
     6481 gtcgaacatc tgccgcggga tcaggccgcg cagcttggcg gcgagctcgc ggccgcgata
     6541 ctgcgcgctg gcgcggtgca cgatgacgga cagcgcgtcc accttctcgc cgcccaccat
     6601 gaggtcgagc ttgacgaggt cggccgtgcg gtactccttg aaatcgtagt cgagcgaggc
     6661 gtagccgcgc gacaccgact tgagcctgtc gaagaagtcc atcacgactt cgttcatggg
     6721 catctcgtag atcagctgca cctggcggcc gtggtagcgc atgtcgatct ggttgccgcg
     6781 cttctggttg cacagcgtga tcaccggtcc gacgtagtcc tgcggcagga aaatggtcgc
     6841 ggtgatgatc ggttcgcgga tctcctggta cttgccgggc tccggcagct tggccggatt
     6901 ctcgatgcgg atgatcgagt tgtcgttcag taccacctcg tagaccaccg tcggcgcggt
     6961 ggtgatgagg tccatgtcga actcgcgctc gaggcgctcc tgcacgatgt ccatgtgcag
     7021 caggccgagg aagccgcagc ggaagccgaa gccgagcgcc tgcgagacct cgggctcgaa
     7081 ctgcagcgag gagtcgttca agcgcaactt ttcgagcgag tcgcgcagtt ggtcgtattc
     7141 ggaagattcc acaggataca ggccggcgaa gacctgcggc ttgatctcct tgaagccggg
     7201 cagcggcgca ctcgccggct tgttggcgag cgtgatcgtg tcgcccacct tggccgcctc
     7261 gatctccttg atgccggcga tgacgaagcc cacctcgccc gcgcgcagcg catcccggcc
     7321 caccgagcgc ggcgtgaaca cgcccacctg gtcgcaggga tactgcaagc cgttcgacat
     7381 cagcaggatg cggtccttgg gcttgagttc gccgtcgacg acgcgcacca gcatgaccac
     7441 gccgacgtag ttgtcgaacc aggagtcgat gatcagcgcc ttcagcggcc cgtcgggctc
     7501 gccctggggc ggcggcaccc gggcgacgat ggcctcgagc acctcctcca cgcccaggcc
     7561 ggtcttggcc gagcacagga tggcctcgga ggcatcgacc ccgatgacgt cctcgatctc
     7621 gccgcgcgcg ttctcggggt cggccgcggg caggtcgatc ttgttgagca ccggcaccac
     7681 ctcgacgtcg aggtcgagcg cggtgtagca gttggccacg gtctgcgcct cgacgccctg
     7741 cgaggcatcg accaccagca gcgcgccctc gcaggcggca agcgagcggc tgacctcgta
     7801 ggagaagtcg acgtgtcccg gcgtatcgat gaggttgagg ttgtagacct gcccgtcctt
     7861 ggccttgtag tgcagggccg cggtctgcgc cttgatcgtg atgccgcgct cgcgctcgat
     7921 atccatcgag tcgagcacct gctcctccat ctcgcgctcg gacaggccgc cgcagaagtg
     7981 gatgagtcgg tcggccaggg tcgatttgcc gtggtcgatg tgggcaatga tggagaagtt
     8041 tcggatgtgc ttcatttgaa acgaaaaagg tgcctttcag cacccgtttc ggaaaggatt
     8101 cggttagtcg ccgggcgggt tcggtccagc gcgtcaggcc cgcgattcta gcggaaaacc
     8161 gcccagataa gcgcgtaccg cggcctcgtc gaggtgatag tggcaaagtt cgtgctcccc
     8221 gtgcagcagc acgggcaccc attcacccca gcgcgcctcg agctcgggat gctggtcgac
     8281 gtcgatcacc tcgatgcccc agccggccgc ttcggcgagc gggcgcaggg cgtccacgag
     8341 cacatggcac aggtggcacc attcccggct catcacggta aaacgccgtt ccatcgcgac
     8401 cgggctcact tgtcggcgcg caggctgaca taggacgcgc tctcgccgcg cccgaccagc
     8461 agggtgacct gctgcccggg cttgagtgcg gcgaccacgc gttcgaaatc ctcgatgcga
     8521 tcgagcctga cctggcgccc ttcgacgacg atcgccagca cgaggtcgcc gggaacgatc
     8581 tgggcccgcg cggcggactt ttcggcacgc tgcaccaaca ggccgtgcgc aagcccgcgc
     8641 tcgcgccgct gctgcgccgt gggcgcgacg agttcgaggc cgaggcggtt ggtcgcgccc
     8701 gtggccagac cgaccgccac gggctcgacc tcttcctccg ggtcgcgcca ttcgcccagc
     8761 gtcaagctca gggaacgcgg cgccccgtcg cgatagatct cgacctcgac cttgctgccc
     8821 gggcgcgccg ccgcgacgat gcgcggaagg tcggccgaat tctcgacgtt gcgctggttg
     8881 aagcgcacga tcacgtcgcc ctggacgacg ccgccgagcg ccgccggacc accggcctcc
     8941 acactgctca ccagcgcacc cgcagggcgc ggcagcccga aactgtcggc gaggtcgcgg
     9001 gtgatctcct ggatcgacac cccgatgcgc ccgcgctcca ccctgccctt ctcgcgcagt
     9061 tgctgctgca cgtccatcgc gacatcgatc gggatcgcga aggacagacc catgaacccg
     9121 cccgtacgac tgtagatctg cgaattgatg ccgatcacct cgccacgcag gttgaacagc
     9181 ggcccgcccg aatttcccgg attgatcgcg acgtcggtct ggatgaaggg cacgaagttc
     9241 tcgtcgggca ggctgcgccc cttggcgctg acgatgccgg cggtgaccga ctgctcgaag
     9301 ccgaagggcg aaccgatcgc cagcacccac tcgcccaccg cgagcgcctc ggggtcgccg
     9361 agcacgacat gaggcaggtc cttggcctcg atgcggatca gcgcgacgtc gctgcgcgca
     9421 tcggcgccga tcaccgtggc gtcgaactcg cgcttgtcgg cgaggcggac cacgatgctc
     9481 tctgcggcct ccaccacatg cgcgttggtg aggatgaaac cgtcggcgct gatgatgaag
     9541 cccgagccga gcgacttgtc gtcgggctcc gcccccgggt actcgggcat gcgcgggatg
     9601 aacttgcgga agaactcgaa catcggatcc gactcgtcga gttccggaag acggaagggt
     9661 tgcttctgcg gctgcgcagc cacctgggtg gcgctgatat tgaccacggc ggcgccctgt
     9721 gtcgccacga ggcgggtgaa atcggggagc atcgcccgct ctgccgtgcc ggccgcgaag
     9781 cccctcggcg cgcagagcat cagcaggaga cagagcacca cggcgagcgc cctcgcggcg
     9841 ctcgtttgca tgcggacttc ggggttcatt gcaggacgcg cagccggcgg ctgcaattct
     9901 cctcgaccga gagctccatg ctgagactgt tccgccagtt gcggctgcgc gacagcatcc
     9961 gattgacgac ccaggccagc gcgagccccg cgacgccccc ggccagaccc caggcatcgc
    10021 cgtgctgcac gccgcccaag aggctgccga acgcggcgcc gaccagaagc agcagcgtcc
    10081 ccagcccgta gctgaccagc gccgcgcgca gcggcgcacc gtccgggatg cggatcacca
    10141 ccgcatcgcc agctgcgaag cgggcgtccg cggggatgga gaattcgtcc cggggcatgc
    10201 cgaagacgtg ggagatctgc accgagccgc aaccacccgg ctcgtcacag cgtccgcagc
    10261 cgccctgctt gccactgagc ttgacccagg cctgaccatt ctcgatccgc aggatacggg
    10321 cgcgcgcttc catcatcgtg cctccggccg aatgccctcg ccgatgcgac gcaccgccgc
    10381 ctctggcgcc tcgccgagtg cggtgacaag gtgaccgtcg atcgcgcgct tgaaaatgtt
    10441 gatcgcgccg ctggagagcg ggccgaggcc ggctcgcgaa ccctcgccga tcggttcgat
    10501 gaacacggag atcgccgcca gcccgtcgct gaaaaccatg tgcaccgctt caccgcgatc
    10561 ccgccccagc gggcgcttca tggccgaggt gagcgcgtaa cccggaaccg ccggctcgag
    10621 cacccagccg gcagcctcga cggacatgct gacaccgcgc gcattgacca tccgccagcc
    10681 ttcgtcggtc ttgaagcgcg actcgagcag gctgttctcc acctcgcccc cgatgcgcag
    10741 ctcgctgaag ctgaattgct cgatgacagc accgtgctga tcgagcgtac gcgacttcag
    10801 cagcaggccc gactgcacgt cggcccacag cacgtggccg tagcgcaatt cgtccttggg
    10861 ctcgaggacc agcgcctggg cctcgagccc ggccaccctg cccaattcgc ccttgcgcac
    10921 acggtagttt tcggccagcc cggtcggcga cgccggcagg cgagccggga aggcgcgacg
    10981 gccctgcgct tcgtcgatga tcaccgtgcg ctcgtcgggc aatacgcaac gcacctcggc
    11041 gccacggcgg atgatttcgc gcggactgcc atcgaggacc tcgaggcgct cgtactcgcc
    11101 gctggcatcg acacgatgcg cgatccgcga ggtctcgaca tggctgccgg actgatacac
    11161 gaaggtgcca acgtaattga gtcgttgccc cgcggtcgag atgcgtccga gccaggacaa
    11221 ggggtcggtc gggtgttcgg cagacgccgg tccctgcgcg agcagcgcgc agagaccgag
    11281 catggcgggg aaccacctca tcgagcgccg ccctcgcgcg tgtcggatac ggtgcggacg
    11341 tactgcacgc cggccggcat cgggccgccg cggctgaggc cctgatgggc gaagagatac
    11401 tcgcgcatcg gatcggccgc cggagcggcg accgggcgca cattcgagga accgacgaac
    11461 tggatcggcg acgcggcgac cgccatctgc ggcgccggcg cgtcctgccg gtacagactc
    11521 gcggccacca accccaccgc ggccaccccc atcaccgaag cggccagcgg catcaggcga
    11581 tgcacgaggc cgcgctgcgc gaggcctgcc gccgggaccc caggcgcgag cagcgtcggc
    11641 tcgtcatcca ggctgaccat gacacgggaa acgaaatcac ttgaaccggc ggagtcgcca
    11701 cgcagggtgt cgccgatcag gcaataggtg tcccactcct tgcgcagcga agcatccttg
    11761 cgcatgccgt cgaacaccgc acgcatgccc tggtcgtcga gatctccgtc gaacagcgcc
    11821 gacactttgt ccttcatgct tgcacctacc aacgtttgtc cggggccgtg tccagcaggg
    11881 gccgcagacg ttctgctatc gcttcgcgcg cacggaagat ccgcgagcgc accgtgccaa
    11941 tcgggcattc catgatgccg gcgatctcct cgtaactgag cccttcgagc tcgcgaagca
    12001 tgatcgccgt cctcagttcc tcgggcagtg cggccatcgc gtcctccacg gtttcgccga
    12061 tctggcgggt catcagcagc cgctcgggcg tgttgatgtc gcgcaactga tcgccttcct
    12121 cgaaggtctc cgcctcttca gaatcgaaac tcgtcgaggt cggggccctg cgcccctggg
    12181 agaccaggta gttcttcgcc gtgttgatgc cgatgcggta cagccaggtg tagaaggcac
    12241 tgtcaccccg aaacgaaccc agcgctcgat aagccttgat gaaggtctct tgcgccacgt
    12301 cctcgacttc tgccgagtcg cggatcagcc tcgacagcaa tctgttcagc ttgcgctggt
    12361 acttggatac cagcagaccg aacgcctgct tgtcgccacg ttgaacacgc tcgacgagtt
    12421 gctgatcgag ttcccgatcg ctcatgggtt cctgttgatt ccggggaatt caaagattcg
    12481 aagatcccgg attataagcg agcgggccac gtactcagca gaaatcgaag acactggaac
    12541 tgacagggtc gggtggaaat agttcaggcg cgcggacggg caggacggac gaccgggcgc
    12601 gcccgacggc ctgtcaccgc atgctatatt ccctctcccg caaatcctgc ccgagcgaca
    12661 tcttgctgaa acagtacgac gtactcattc ttggcagcgg taccgccggc cagtcgatgg
    12721 ccctccgtct ggcggaccgc ctgcgcgtgg cgctcgtgac caagcgcgag ttggctgaca
    12781 gcgcgagcaa ctgggcgcag ggcggcatcg ccgcggtgct cgacaacacc gacagcatcg
    12841 aagctcatat ccaggacacc ttcgtcgccg gcgccggcct gtgcaacccc acgtcgacgc
    12901 gcttcgtggt cgagaacggc aagcgcgcga tcgaatggct gatcgaccgc ggcgtaccct
    12961 tcacccgcga ggccgactcc cagctcggct accacctcac ccgcgagggc ggccattccc
    13021 accggcgcat catccacgcc gcggacgcca ccggcgcggc ggtgcaggcg acactcggcg
    13081 accacgtgcg ccgacacccg aacatcgaca tctacgagca ccacatcgcg gtcgatctca
    13141 tcaccgcgga caagctgggg cagtccggcg caggctgcct cggcgcctac gtgctcgaca
    13201 tccacggcga gcgcgtgctg accttcgccg cgcgcaacac cgtgctcgcc accggcggca
    13261 ccggcaaggt ctatctctac acgaccaacc cggacgtcgc caccggcgac ggcgtcgcga
    13321 tggcgtggcg agcgggctgc cgggtgggga acatggaatt catccagttc caccccacct
    13381 gcctgtacca cccgcaggcg aagtccttcc tgatctccga ggcggtgcgc ggcgagggag
    13441 gcctgctgaa gctgcccgac ggcacgcgct tcatgcccga gcacgacccg cgcgaggagc
    13501 tcgcaccgcg cgacatcgtc gcgcgcgcga tcgatttcga gatgaagaaa cgcggcatcg
    13561 actgcgtcta cctcgacatc agccacaagc cggccgactg gctgcgcggc cacttcccca
    13621 atatccacgc gcgctgcctg gagctgggca tcgacatcac caaggagccg atcccggtcg
    13681 tgcccgcggc gcactactcc tgcggcggca tcatgaccga ccttgccgcg cgcaccggcg
    13741 ttcccgggct ctacgccgtg ggcgaatctg catgcaccgg cctgcacggc gccaaccgcc
    13801 tcgcgagcaa ctcgctgctc gaatgcctgg tgttcggcga agccgcggcc gaggacatcc
    13861 tctccaaggc gcaagagcct gcacccgtgc ttccggcctg ggacgagagc cgcgtcaccg
    13921 acgccgacga ggaggtggtg atctcgcaca actgggcgga actgcgccgc ttcatgtggg
    13981 actacgtcgg catcgtgcgc accaacaagc gcctgcgccg cgcccagcac cgcatccgcc
    14041 tgctgtcgcg cgaaatccac gagttctact cgaacttccg cgtcagcaac gacctgatcg
    14101 agttgcgcaa tctggtgacc actgcggacc tgatcgtgcg ctgcgcgctc aagcgcaagg
    14161 aaagccgcgg cctgcaccac tcgcgcgact atcccgagac acttccacgc gcacgcgaca
    14221 ccgtgcttcg tccgccggcg cgcacgcccc gcaaacgctg agcgccagcc ctgtcgcgcc
    14281 ggcgcgatcc gtccgcggac gagaccgcgt cagcgcgcag cggcgtcggg cccgacgcgc
    14341 cccgagcgat ggcgcagcca gaccctgagg aggcggaaac tgccggcgga tagattgccg
    14401 ggaacgagga acagcgttcg agcaaccggg gcgccctgcc ccggcgccga tatctgcgcg
    14461 ggcaccagcg tgaaccaggc ggccatcggc aacaccacct gcgagcgttc gcgcacccga
    14521 tacagcccac cttcaccgtc cggttcctgc aacagttcga gcgcgccgtc gcgctccagc
    14581 cagagcgtgc agccatccag acgagcctgc gcacgcaagc cgcgaaacag cgacaggaag
    14641 atcagcgccc aggcgcaggc ggcgatcgcc cgatccacgt ctcccgcctc ccagtccgtg
    14701 aagacaggca cgtgaaaaag cgccagtgcg gccgctgcat gcacgaccca caccgacgct
    14761 ttcacgagac tgcagccgcg cagcttcagt tcgacgggga actgcatgcc gcggagcaga
    14821 acgaaaggct cacacccggc ggaacaccag ggtgccgttg gttccgccga agccgaaatt
    14881 gttcttcagg gccacgtcga tcttcatctc gcgggcgacg ttggcgcagt agtcgaggtc
    14941 gcactccgga tcctgctcga agatgttgat cgtcggcggc gaaatctggt ggtacaccgc
    15001 cagcgcggtc acaaccgact ccagcccccc ggcgccaccg agcaggtgac cggtcatcga
    15061 cttggtggag ttcaccacca gctgcttcgc cgcttcgacg ccgaaggcga gcttgatcgc
    15121 gtccgtctcg ttcttgtcgc cgagcggggt cgaggtaccg tgcgcgttga ggtactgcac
    15181 ctgatcgacg ttcacgccgg cgtccttcat cgcattgacc atgctgcggc gcgggccgtc
    15241 ggtgtcgggc gcggtcatgt ggtaggcgtc gccgctcatg ccgaagccgg caacctcggc
    15301 gtagatcttc gcgccccggc gcctggcgtg ctcgtactcc tcgagcacca tcacgccggc
    15361 accctcgccg agcacgaagc cgtcacgccc cttgtcccac ggccggctgg cggtggcggg
    15421 atcgtcgttg cgggtggaca gcgccttggc cgagccgaag ccgccaatgg cgaggttggt
    15481 cacggtggat tccgcgccac cgcacaccat cacgtcggca tcaccgtagc cgatcatgcg
    15541 cgcgcccatg ccgatcgcgt gcaggccggt tgtgcaggcg gtgaccaccg cgaggttggg
    15601 ccccttcatg ccgaacatga tcgagaggtt gcccgagatc atgttgatga tggtgcccgg
    15661 aatgaagaag ggcgagatct tgcgcgggcc gccaccgagg aagtcgttgt gcgtatcctc
    15721 gatcatcggc aggccgccga tccccgaacc gatattgatg ccgatgcggt cggcgttggc
    15781 ctcggtgacc tcgatgccgg agtcgcggat cgcctggatg ccagcggcca tgccgtagtg
    15841 gatgaacaca tccatgcggc gagcttcctt gggcgacagg taggcggaca cgtcgaagtc
    15901 cttcacctcg ccggcgatgc gggcagcgaa gttgctcgcg tcgaaacggg tgatcggacc
    15961 gatgccgctc ttgccggcga tcaggttgga ccaggcttcg gtaacagtat tgccgacagg
    16021 cgagatcacg cccagaccgg tgacgacgac tctacgacgc gacacaggcg actccgttga
    16081 aatcgttgga gaaattcaaa cctgtccggt tgccgcaacg atgcgcggta acgcggacag
    16141 gacgcgcggg aaaatgcgcg gggattactt cttgaggtgg gcgttgacgt agtcgatcgc
    16201 ctgctggacg gtggtgatct tctcggcctc ttcgtccggg atctcgcact cgaattcttc
    16261 ttccagcgcc atgaccagtt ccacggtgtc cagcgaatcc gcgcccagat catcgacgaa
    16321 cgaagactcg gtcttgatct ccgattcgtt cacgccaagt tgctcagcga cgatcttctt
    16381 gacgcgctgt tcgatgttct ccatgaacca actccttcct gagggtgaca ggtgaatgta
    16441 aaaagccggc gtattctacc aaaacgccgc ggaaccgcta tcccgacgca aatcaggaca
    16501 tgtacatgcc gccattgacg tgcagggtgg tccccgtcac gtaggcggca gcgggggagg
    16561 cgaggaacgc cacggcgccg gcgatttctt ccggacggcc gagacggccc agcgcgatgt
    16621 tgccgagcaa ggcgtcgcgc gcggcttcgg gcagcgcgcg cgtcatgtcg gtgtcgatga
    16681 aaccgggggc gacgcagttg accgtgatgt tgcggctgcc gagttcgcgc gccagcgcgc
    16741 gcgtcatgcc cgccacgccg gccttggcgg cggcgtagtt ggcctggccc gggttgccgg
    16801 agctggccac caccgaggtg atgttgatga tgcggccgtg gcgggccttc atcatgccgc
    16861 gcatgaccag cttgctcatg cggaagaccg cgcgcagatt ggtgtccacc accgcatccc
    16921 actcctcgtc cttcatgcgc atggcaaggt tgtcgcgggt gatgccggca ttgttgacca
    16981 ggatgccgac cgcaccgaag cgcttctcga cctcgccgac cagcgcctcg caggctgccg
    17041 cgtcggtcac gtccagcgcc atgccggccc cctggatgcc ggcgtcggcg agcgccttgc
    17101 cgatatcggc ggcgccggat tccgaggtgg cggtgccgac caccgtcgca cccaggcggc
    17161 cgagttcgag cgccaccgca cggccgatgc cgcgcgacgc gccggtgacc agggcgacct
    17221 ggccttcgag cgaaaaactc atgtcttacc tcaccgccgc aatggcttgt tccaggctcg
    17281 ccgcgtcatg cgccgagcca ccctcgactt ccttggcgat gcgcttggtc atgccggcga
    17341 gcaccttgcc ggggccgcac tcgatgacgt ggctcatgcc gcgggcgccc atcgcctgca
    17401 cgagctcgat ccagcgcacc ggcgaatacg cctgccgcac cagggcgtcg cggatcttct
    17461 cgggatcatc gtaaaccgcg acgtcgacgt tgttgatgac gtcgatctcc ggcttgacga
    17521 tcgccacacc ggccaagcgc tcggccaggc gctcggcggc tgggcgcatc agcgcgcaat
    17581 ggaagggcgc ggaaaccggc agcagcacgg cacgcttggc gcccttggcc ttggcgatct
    17641 cgctcgcgcg ctcgaccgcg gccttggcgc ccgcgatcac gatctgcccc ggcgcgttga
    17701 ggttggctgc ctcgaccacc tggccctgcg cggcctcggc gcaggccgca tgcaccgcgt
    17761 cgggctccag gcccatcacc gccgccatcg cgccctctcc cgccggcacg gcctcctgca
    17821 tggcttgggc gcggaagcgc accagcggca cggcgtcctt gaaggccagc gcgcccgcgg
    17881 cgaccagcgc cgagtactcg cccaggctgt gaccggcgac cagggccggt ttcggcccgc
    17941 ccgcgccccg ccaggcacgc caggccgcca cgccggcggt cagcatcagc ggctgggtgt
    18001 tgacggtgag ggcaaggcgc tcggcagggc cgtcgcagac catcgcccag agatcctctc
    18061 cgagggcctc ggaggcctcc gcgaaggtgt cacggatgac ggcggactcg ccgtaggcgg
    18121 ccatcatgcc gaccgattgc gaaccctggc cgggaaaaac cagcgcaaaa gccatgctgt
    18181 tctccgcttg tgtcgaaagt gggaaatcgg gaaacgaagc tcagaaatcg atcagggccg
    18241 cgccccaggt gaagccgcca ccgacgccct cgaccacgat gcgatgcccc gggcgcaacc
    18301 gccccgcacg caccgcctcg tcgagcgcga gcggaatcga cgccgccgag gtgttgccgt
    18361 gatgatcgag cgtggtcacg acgcgctcca tcggcacacc gagccgcttg gcggtggcct
    18421 ggatgatgcg gatgttggcc tgatgcggga tcagccagtc gacactggac gcctgcacac
    18481 ccgcggcatc cagcacctcg ttggccacat cgccgagcac cttgaccgcg aacttgaaca
    18541 cggcctggcc gtccatgcgc aggaagggat cgccgatcac ctgccccgag gccacagcgc
    18601 ccggcacgca caggatgggg tggtggctgc cgtcggcgtg cagcgccgaa gccaggatgc
    18661 ccggccgctc cgaggcctcg agcacgaccg cgcctgcgcc gtcgccgaag agcacgcagg
    18721 tgccccggtc ggaccagtcg aggatgcgcg agaacacctc ggcgccgatg accagcgcgc
    18781 gcttgttgct gcccgagcgg atgaacttct cggccacgct gagcgcatag atgaagccgc
    18841 tgcacaccgc ctgcacgtcg aaggcggcgc cgccgttgcg gatgccgagc ttggactgca
    18901 cgagggccgc cgtgctcggg aagatgtagt cgggcgtgga ggtggcgacg atgatgaggt
    18961 cgatgtcctc cgcttcgcag ccggcggcct cgatcgcgcg ctcggccgcg acgcgggcga
    19021 ggtcgctcgc cccggtgtcg gccgccgcca gatggcggct gcggatgccc gagcggctga
    19081 cgatccactc gtcggaggta tcgatgccac gcttgaccag gtcgtcgttg ctgaccggtt
    19141 cgccgggcag gtagctgccg gtaccggcaa tccgtgcata gatcatgcct tcgcctcgtt
    19201 catgctcgcc atgcgttcgg agatgcgctc gatgagccgg ttcgacgccg cttcggcagc
    19261 gcgcgaaagc gcattgccga atgcgagcac gtcggccgag ccgtgactct tgagcaccac
    19321 gccgcgcagg ccgagcagcg gcgcaccatt gtaaagacga tgatcgacgc gcttcttgaa
    19381 gcgcttgagc accggcatcg ccgccaccgc cagcagcttg gtgaacagcg tgcgcttgaa
    19441 ctcctgcttc aggaaggcgc cgaccatctg cgccagaccc tcggaggtct tgagcgcgac
    19501 gttgccgacg aagccgtcgc agacgaccac atccaccgtg ccttcataga tgtcgttgcc
    19561 ctcgacgttg ccctggaagt tgaggccgct gttacgcagc agctcgcccg cttccttgac
    19621 gacgtcgttg cccttgatga cctcctcacc gatgttgagc aggccgaccg taggacggtc
    19681 cttatgctcg atcgcagcga cgagcatcgc gcccatcaca ccgaactgga gcagatgctc
    19741 ggcgctgcaa tcgacgttgg cgccgaggtc gagcacatac gtgtgcccct tcatcgacgg
    19801 cagcacggtc gcgatcgcag ggcgatcgat cccgggcagg gtcttcagca cgaagcgcga
    19861 gatcgccatc agcgcgccgg tattgcccgc cgagacggca gcgctcgcct cgcccgcctt
    19921 gacgaggtcg accgcgacgc gcatcgacga atccttcttg ttgcgcatgg cgatcgcggg
    19981 cggatcgtcc atggccacca cctcggaggc cgcgcgcacg cggatgcgcg cgccgaactc
    20041 ggtcgtcgcg ttgccgagca ggggcgcgat cgcctcctcc cgcccgacga ggatcgcgct
    20101 ggcctgggga tggctgcgca ggaattccag cgcggctggc acggtcacga ccgggccgtg
    20161 atcgccaccc atgcagtcga tcgcaatggt gacacccatc gggttctcgc gtgattacag
    20221 gtttcgggag ccggaaaaaa ggcggcgccg gcgcaatgcg cccgacgccg cccttctcga
    20281 ttcaggcagc aggattactc gcccttgccc ttggcgacct tcttgccacg gtagaaaccg
    20341 ctggggctca cgtggtggcg caggtgggtc tcgccggtgg tgggctcgac ggccagcgcc
    20401 ggaccggtga ggaagtcgtg ggcacggtgc atgccacgct tggacgggga cttcttgttc
    20461 tgctgaacgg ccatgagtaa agctcctaaa acaaatatcg cgcctatttc gcgctgtgac
    20521 tgcctcgcag cgacgccagc gcagcgaaag gcgattcctt cgacgcacca ccatcgggcc
    20581 gtggcgcgtc acaaacctgg tgtcgcgggg cgatcggcag ggcgagcagg atctcgtcct
    20641 cgaccagggc gatcacgtcg agctcgccat cgagctcgat cgcatcgatc tcgtcatcct
    20701 cgagctcttc ctcggggatg ggctgacccg aacgcaccgg ctgcagcacc gagtccacct
    20761 ggaccggcca tgccatcccg ccgaggcagc gctggcagca aagctgcagc tcgccctcga
    20821 tcatcaggtg cagccgcggt tcacgcgcct cgacgccttc gaccatcgac ccctcgatcc
    20881 gccacgacac ctgcccgccg gacgacgcga gcacgtcgga cagacgcagc atctccgcga
    20941 cagcggtcac gccttccagc gtgcgcgcct ccgcggcaaa tcgaaacggg tcggagatga
    21001 tcctggatgg atcgccatgt tgcgacataa gccgcgcatg ataatatttt tcggatcggc
    21061 ctgtaaaggc ttcattcgat tcgggaagcc ccaccccgat ccctcctctc cagcacagtc
    21121 gcgcgaacaa ctcgaccgat ctccggcggg tcatgttcgc accggaccca tgaagatcgt
    21181 cctcgcctcc acctccgcct accgccggat gctgctcgag cgcctgcaac tccccttcga
    21241 gaccgaccgc ccggagacgg acgaatcgcc tctgcccggc gaggcacccg ccgccaccgc
    21301 cgaacgcctg gcgctggaga aggcccgtgc cgtcgccgcg cgctggcccg gcgcgctgat
    21361 catcggctcg gaccaggtcg cccacctcgg caacgaggtc ttcggaaagc cgggcaccga
    21421 ggcgcgtgcg atcgcgcagt tgcagcgcat gcgcggacag accgtggtct tccacaccgc
    21481 gctcgccctg ctcgacaccc gcagcggcca cgcccaggtc gagggcgtgc cgacgcacgt
    21541 ccgcttccgc gaactctccg acgacgagat ccgccgctac gtcgagcgcg aacggccgct
    21601 cgactgcgcc ggcagcgcca agtccgaagg cctcggcatc gcgctgctcg aagcgatgcg
    21661 gggcgacgac cccaccgcac tgatcggcct gccgctgatc gccctcgcgc gcatgctgcg
    21721 cacggccgga gtgcatctcc catgagcggc gacgccatgc cgcgcggcaa cctctacctg
    21781 gtccccgtgg ccctgggcga gcacacaccg tgggcacact tcctgcctgc gcaggcacag
    21841 gcgatcgcag ccggcctgcg ccacttcgtc gtcgagaccg cgcgcgcggc gcgcgcccac
    21901 ctcaagcagc tcgactatcc ccacccgctg cgcgacacgg acatccgcga gctgcccgcc
    21961 gaggccgccg acgcggcgct cgacgcgctg ctcgcccccg cgctcgccgg cacggacctc
    22021 ggcctgctct cggacgccgg ttgcccggcg gtggccgacc ccggcgcccg cctggtggca
    22081 cgcgcgcacg ccctcggcat ccgcgtcgtg ccgctggtcg gcccctcctc catcctgctc
    22141 gggctgatgg gctcggggct caacggccag agcttcgcct tccacggcta cctgccggtg
    22201 gccgagggcg agcgcgatgg ggccctgcgc acgctcgagg acgaatcccg caggctcggg
    22261 cgcacgcaga tcttcatcga gacgccttat cgcaacgagc gcatgctcga cgccctgctg
    22321 cgcacgctca agcccgaaac ccggctgtgc gtggcctgcg aactcaacac cgcggacgaa
    22381 ttcctcctca cccgcagcgt gcgcgagtgg aagaaggctg cgcgccccgc cctcgcccgc
    22441 cgccccgcgc tcttcctgct gctggcctga acgacgctac gcggctcagg cgttggccag
    22501 cagccagccc gcgagctcgc ccggcgtacg cacgcaggcg agcggggact cggccaccag
    22561 cacctcgacc ggatgcgcgc cgaagcccac cgccagcccg gcggtgcgcg cgttcttcgc
    22621 catctgcagg tcgtgggtgg tgtcgccgat catcagggtg cgctccggcg ccacgccgag
    22681 ctcgtccatg agcgcttcaa gcatcgccgg atgcggcttg gagaagcact cgtcggcgca
    22741 gcgcgtggca tgaaaatagc gacccagccc ggtgtggccg agcgcacggt tcaggcccac
    22801 gcggctcttg ccggtggcga ccgccagcag gtgaccgcgc ccggcgagcg cgtcgatcag
    22861 cgcgtcgacg ccctcgaaga ggctcagctc gtgatccgag gacaggtagt ggtgacgata
    22921 gcgctcgacc atgcgcggat agtcggactc ttccagatcg ggcacggcgt ggcgcaacgc
    22981 atcgcccagg ccgaggccga tgacgtagcg cgcgcgctcc tcggagggca ccggcagatc
    23041 gaggtcgcgc gccgcggcct gcatcgcacg cacgatggcg gcagccgaat ccatcagggt
    23101 gccgtcccag tcgaagacga tcaggtcgaa acgttcagcc atgctttttc tccgcggcat
    23161 cgagccggtc gaggaaggtt tgcaggtccg cgggcagcgg cgaatcgaag gcgagcggcg
    23221 caccggtgag cgggtgggcg aggcgcagct tcgcggcgtg caggaacatc cgccgcaagc
    23281 cctcgacctc caggcgcttg ttgagcgcga agtcgccgta tttgtcgtcg ccgcacagcg
    23341 cgaagccgag atgggcaaga tgcacgcgga tctgatgcgt gcgcccggtc ttcagttcca
    23401 cctcgagcag gctgtagtcg cgccagcgcc gtaccaggcg cacgatgctg tgcgccgcct
    23461 tgccctcgtc cgcgacgcgc acccggcgct cgccttcggc ggtcaggtac ttgtgcagcg
    23521 gaaccttgac gtgctgcagc ggattggacc agcgccccgg caccagggtc agatagcgct
    23581 tctccacgct gccctcgcgc atcatgtcgt gcaacgcggt gagcgccgag cgtttcttcg
    23641 cgacgatcag cagccccgag gtctcgcggt cgaggcgatg ggcgagctcg agcatgcgta
    23701 gctcggggcg ctgcacgcgc agttgctcga tcaccccgta gctcaccccg ctgccgccat
    23761 gcaccgcctt gccggagggc ttgtcgatga cgagcagcgc ctcgtcctcg tacaccaccg
    23821 gcagcggctt gcccgccggc gcgggtgcgg cgtgggtcgg ctcggcgatg cggatcggcg
    23881 ggatgcgcac ctcgtcgccc tcctgcaggc gataggtctg ctgcacgcga cgcccgttca
    23941 cccgcacctc gccgctgcgc aggatgcgat agacgtggct cttgggcacc cccttggcga
    24001 tgcgcaggag gtagttgtcg atgcgctggc ccgccgccgc ctcgtcgatg cgttcgtggc
    24061 gaaccgcgat cgctttgcct tcaatcgtcg tcatgaaata tactttcgcg cggttgaacc
    24121 ggtcgctgcc acccaagtgc ggcgcgtcag cagtccgtca ggtcgaaaca cctgctccgg
    24181 acgacgcgcg cagactgctg gcaaagagcc ctgaagagcg ctcccggtca caacccttgc
    24241 ccgccatcca aggccgaaaa ggcgccgatg gtaacgcaag cgatcccctt cgacacaccc
    24301 atccagcccc cgggttggat tttgacgcgc aagtcaccga cgcaggatca aatgttccgg
    24361 ccacgctgcc gcaccggcac cgcgggcggg aacaagagaa attcgaccag ccgacccatc
    24421 acagccctgt taacccaccg gatgaaagca gcagcagccc tacccaatcg ctcctgatcc
    24481 cggaaagcgc gatggtgcgc gccgttcgtc ctgcccgtgt gtcttctcgc gggagaacga
    24541 tccaaatgaa gcgcatgctt ttcaatgcga cgcaggccga agaactgcgc gtcgcgatcg
    24601 tggacggtca gaaactgatc gacctcgata tcgaatcggc cgccaaggaa gaacgcaaga
    24661 gcaacatcta caaggccgtc atcacccgca tcgagcccag cctcgaggcg gcgttcgtgg
    24721 actacggtgc cgaccgccac ggcttcctgc cgttcaagga gatctcgcgt tcctacttcc
    24781 agcctggtgc ggacgccaag gcctcgatca aggaagccct gaaggaaggc caggaactca
    24841 tcgtccaggt cgagaaggac gagcgcggca acaagggtgc ggcgctcacc acctatatct
    24901 cgctggccgg ccgctatctg gtgctgatgc ccaacaaccc gcgcggcggt ggcgtctcgc
    24961 gccgcgtcga gggtgacgag cgcgccgaac tgcgcgagaa catggaccag ctcgacgtcc
    25021 cccagggcat gagcctgatc gcgcgcaccg cggcgatcgg ccgcagcgcc gaagagctgc
    25081 agtgggacct caactatctg ctgcagctgt ggaccgccat cgaaggcgcg gcacagggcc
    25141 agtccggcgc cttcctgatc taccaggaag gcagtctggt gatccgcgcg atccgcgact
    25201 acttccagcc cgacatcggc gagatcctga tcgacaccga cgacatcttc gagcaggcca
    25261 cgcagttcat ggcccacgtg atgccgggca acgtcgcccg cgtgaagcgc tacagcgacg
    25321 acgtgccgct gttctcgcgc ttccagatcg agcaccagat cgagtcggcc tattcgcgcc
    25381 aggtgaacct gccctcgggc ggtgccgtgg tgatcgacca taccgaggcg ctggtgtcga
    25441 tcgacgtcaa ctcgggccgc gccaccaagg gctcggacat cgaggagacc gccttccgca
    25501 ccaactgcga ggcggccgac gagatcgccc gccagctccg cctgcgcgac ctcggcggcc
    25561 tcatcgtcat cgatttcatc gacatggaat cggccaagaa ccagcgcgag gtcgagaacc
    25621 gcctgcgcga cgcgctgcgc cacgaccgcg cccgcgtgca gaccggcaag atcagccgct
    25681 tcggcctgct cgagctgtcg cgccagcgcc tgcgcccggc gctcgccgaa accagctaca
    25741 tcacctgccc gcgctgcaac ggcaccggcc acatccgcag caccgaatcg tcggcgctgc
    25801 acatcgtgcg catcctcgaa gaagaggcga tgaaggacaa taccggcgcg gtgcacctgc
    25861 aggtgccggt cgatgtcgcc accttcctgc tcaacgagaa gcgggtcgac atcgcgcgca
    25921 tcgagatgcg ccacaaggtg cagctcatca tcgttccgaa ccgccatctc gagacgccgg
    25981 cgcacgagat catccgcctg cgccacgacc agctcaacac cgaggacatc gccctggcga
    26041 gctatcagat ggtgcagaag ccggtcgaag aggacacccg cctgccctcc aaggccaacg
    26101 agcgcccggc acgccccgag gccgcggtga agggcatcgc ccccgcgcag cctgccccga
    26161 tggctgcacc cgagcccgct cccgcctcgg ccccggcccc tgccgcgccc gagcccaagg
    26221 ggctgatcgc gcgcatcatg ggctggttct ccgccggcaa gggcgccgcg gaaccggtcc
    26281 ccgccccggt cgtggaagag actccgaaac gcgaggcgcg cccgcgtggc gaacgcagcg
    26341 gcgagggtcg ccgcaatggc ggcagcaatg gcggccgtgg ccgtcgcggc gagcgcgacg
    26401 gcgaggcgca gccctccacc cgcggcagcc gtgccgagcg tggcgagagc accgagcgtc
    26461 cgcagcgcgt cgcggccgag cgtgccgagc gccccgagcg tgctgaacgc cccgagcgtg
    26521 gcgagcgtgc cgagcgcagc gaaggcggcc gccgcggtcg cggccagcgc ggcgagcgtg
    26581 cccaggaagg cgccgagcag cgcgcggccc agaccgagga cgatctgctg aaggccgcca
    26641 ccgccactgc gatggtggca gcggccacga cggcggactc cggcacgacc gagaccgttg
    26701 ccagcaccga aggcggcgaa gaggaaggca ccccgtccga gaagcgccgc cgtcgcggcc
    26761 gtggccgcgg ccgtcgctcg ggcgagccgc agggcatcga cggcgccgcc gaagcgggta
    26821 acgaagtcga gaccggggcg acagcgcccg agggcgaact gtcgggaatc gcggcgtccg
    26881 ccgtcgagca ggggaccgtc gagtccgcgc acgctgccag cccggtcacg gccggacagg
    26941 acgcgaacgt cggcgctgcc gccacagccg tggtgcaaac cgctccggcg gatgcgcaga
    27001 tcgccgaact gccgatcgtc ggcggcaccg cggaggacgt cacaccggtc gccctggccg
    27061 cacctcaggt gccgcttgcc ccggtgccgg tcggcgcagc gctggaagtc gtcgctgaaa
    27121 cggcgcctgc cgcggaggtc ccggtcgaca aggcccttgc ggccgaagcg gtgctcgccg
    27181 agcaaggtgc tgctgccgaa gagcttgccg ccgggagtgc tgccacggtc gaagcggtat
    27241 ccgccgcggc gccccgtgcc gagaccgctg ccatgatcga ttcggcttct gccgaggaag
    27301 cgccgcctgc ggtcgcctcc gcagcaggcg aggaggcggt catcggcacc gaacccgtcg
    27361 cggtcgccga gcccgccccc acgtcgagcg ccggcgtccc ccccgaggtc gccgccgcag
    27421 cgccggaagc cgtgccagcc ccgctcgcgg attcgacgga ggtcgccgag ccgtccgtgc
    27481 ctgcggcagc cgagtcgacg gtggttcgcg cgatggaagc cggccgtgcc agccacgcgc
    27541 cgagcgcacc gatcgcggtg ccgtccggcg acagcggcct gatcaggatc gagaccgatc
    27601 cggccaagcg cgtgcagttc ggcaccggcg ccccggagca gccggcacgc ctgggccgcc
    27661 agccccgccc ggccgcagtg atcgccgaag agccgctgca acaggtcgag acccgcaagt
    27721 aagcggttcc accaaccccg gcgcgaaggc ttgtcgagcg cacatcgcga cttgcatcgc
    27781 tcctgcagga aaccagcccg cgtggttgcg ctttccgcag gagcggcgcg agtcgcgatt
    27841 tccttgtcgc cccgaactca gccctcgccc cgaactcagc cctcgccgcg gacgcgttgc
    27901 acgatcccgc gtgctgcgtc ttcacacatg tcgagcacca ggtcgaagcc cttcggcccg
    27961 ccgtagtagg gatcgggcac ctcctcgccg tgagcttgcg cgccggcgaa gggcaggaag
    28021 agctgcagct tgggccggta cacctcgggg caatggcgct gcatgtgctc gagatgcccg
    28081 cggtccatcg ccagcagcaa gtcgaactcc tggaagtcgc gcagctcgag cttgcgcgcg
    28141 cgcagcgccg agaggtcgta gccgcgcagc ttggccgcct tctgcgtgcg cgggtcgggt
    28201 gcctcgccgg cgtggtagcc ctgcgtgccg gcagagtcga gcaacacctg cgcacccagc
    28261 ccgccggtct cgacgaagtg gcgcgccacc gcctcggcgg tcggcgagcg gcagatgttg
    28321 ccagtgcata cgaacaggat cctcttcatc ccctcccctc ctcttcttgt tgttcacacg
    28381 ctgccatgct ggtcggcgcc gaaggcgcgc tcgcgcagct tgaagagctc gtcgcgcgct
    28441 gccgccgcct gctcgaactc caggttgcgg gcgtgctcct gcatctcctt ctccagccgc
    28501 ttgatcgcgc gcgccagcgc cttctcgtcc atcgccgcgt agtcgccgcc cttctccgcc
    28561 acgcgcgcga cgtcgcgctt cgcgccatcg gcatcgtaca ccccgtcgat gatgtccttg
    28621 atgcgcttgg tcaccgactt cggcacgatg ccgttggcct cgttgaaggc gatctgcttg
    28681 tggcgacggc gctcggtctc cccgatcgcc gccttcatcg agtcggtgac gcggtcggca
    28741 tagaggatgg cggtaccgtg cagatggcgc gcggcgcggc cgatggtctg gatcagcgag
    28801 cgctcggagc gcaggaagcc ctccttgtcc gcgtccagga tcgccaccag cgacacttcg
    28861 ggaatgtcca ggccctcgcg cagcaggttg atgcccacca gcacgtcgaa ctcgcccagg
    28921 cgcaggtcgc ggatgatctc gacgcgctct acggtgtcga tgtccgaatg caggtagcgc
    28981 acgcggatgc cattgtcggc gaggtagtcg gtgaggtcct cggccatgcg cttggtcagc
    29041 acggtgacca gcacgcgctc gttcacttcc agccgcttct tgatctcgga cagcagatca
    29101 tcgacctgag tcattgccgg gcgcacctcg accatcggat cgatcaggcc cgtcgggcgc
    29161 accacctgct ccacgacctg gccctggtgc tcctcctcgt acttcgccgg ggtagcggag
    29221 acgaagacgg tctgcggcat aaggcgctcg aattcgtcga acttgagcgg ccggttgtcc
    29281 agcgccgacg gcaggcggaa gccgtagccg accaggttct ccttgcgcga gcggtcgccc
    29341 ttgtacatgc cgccgacctg gccgatggcc acgtgcgact cgtcgatgaa gagcagcgca
    29401 tcctgcggca ggtagtcgat cagcgtcggc ggcggctcgc ccggaccgcg cccggacagg
    29461 tgccgggagt agttctcgat gcccttgcag aagcccatct cgttcaacat ctcgaggtcg
    29521 aagcgggtgc gctgctcgat gcgctgcgcc tccaccagct tgccctcgcg ctggaagaag
    29581 gcgacgcgct ccttcaactc ctccttgatc gcctcgatcg cctgcagcac cgtcgcgcgc
    29641 ggggtcacgt agtggctgga cggatagacg gtgaagcgcg ccagcttgtg cctgaggtgt
    29701 ccggtgagcg gatcgaacag ggtcaggtgt tcgatctcgt cgtcgaacat ctcgatgcgc
    29761 accgcgagct cggcgttttc cgccgggaag acgtcgatca catcgccgcg cacgcggaag
    29821 gtgccgcggc ggaagtcgat gtcggcgcgg gtgtattgca tcgccaccag gcgctggatg
    29881 aggtcgcggt gcgccatgcg ctcgccctcg cgcaggtgca ggatcatggc gtggtagtcg
    29941 accggatcgc cgatgccgta gatgcacgac accgtggcca cgatgaccac gtcgcggcgc
    30001 tccatcaggc tcttggtcgc cgacaggcgc atctgctcga tgtgctcgtt gatcgacgaa
    30061 tccttctcga tgaaaaggtc gcgcgagggc acgtaggcct cgggctggta gtagtcgtag
    30121 tagctgacga agtactcgac cgcgttctcg ggcaggaact ccttgaactc ggcgtagagc
    30181 tgcgcggcca gcgtcttgtt gggcgccagc accagggccg gtcgccccat gcgggcgatg
    30241 acgttggcca tggtgtaggt cttgcccgag ccggtcacgc cgagcagggt ctggtacatc
    30301 aggccgtcct cgacgccttc gcacagcagg cggatcgcct cgggctggtc gccggcgggc
    30361 ggaaacggct ggtgcaggcg gaaggggctg ccctcgaagc cgagcacggt cccgccttgc
    30421 tgcggtgcat cgtttccggc gctattgcct tgagtcgtca tgctaaaatt cccgctccat
    30481 tccggtgcat cgccgcacgg cggcaccgga cgaaaacgcc gattatttcc caaatcgcgt
    30541 gacaaggcac ggcgcaagtc cgttcccgcc acgcgcattc actgccgccc taccggaaca
    30601 actggagtcc tcatgtccgc ttcgatcttc gccgccgtcg agatggcgcc ccgtgacccg
    30661 atccttggcc tcaacgaggc cttcgccgcc gacacccgcg ccgagaaggt caacctcggc
    30721 gtcggcgtgt actacgacga caacggcaag atcccgctgc tcgccgccgt gcgcaccgcc
    30781 gagaaggcac gcctcgaagc catgccgccg cgcggctacc agccgatcga aggccccgcg
    30841 gcctacaaca acgcggtgca gggcctgctc ttcggcaagg actcgcagct cgccaccagc
    30901 ggccagacca tcaccgtcga ggccctcggc ggcaccggcg cgctcaaggt cggcgccgac
    30961 tatctcaagc gtctcgtccc cggcgccacc gtgtacatct ccgacccgag ctgggagaac
    31021 caccgcgcgc tgttcgaatc cgccggcttt ccggtcgaga actaccccta ctacgacgcc
    31081 gccacccgcg gcgtgaactt cgccggcatg aaggccaagc tcgacagcct gcagcccggc
//
LOCUS       DAOQBS010000024        30474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_885_length_30474_cov_15.599888, whole genome shotgun sequence.
ACCESSION   DAOQBS010000024 DAOQBS010000000
VERSION     DAOQBS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 30474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30474
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_885_length_30474_cov_15.599888"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1032
                     /locus_tag="PKN25_04730"
     CDS             130..1032
                     /locus_tag="PKN25_04730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNV90237.1"
                     /translation="MQATPSRAQHATEDERGQFDSLLPAARLDRRGFLAGIAATGFAL
                     AVQPVHASTVISTSGTGLATGTAAIAVEGGELPLYYARPAAGDRLPVVLVVQEIFGVH
                     EHIRDVCRRLAHAGYLAIAPELFFRQGDPTTQPDIPAILQNIVAKVPDAQVMGDLDAC
                     AAWSASQGGDPARLAITGFCWGGRITWLYAAHKPSVKAGVAWYGRLSGAISEFTPQHP
                     LDVVGRLHAPVLGLYGGQDQGIPLADVEKMQAALSAAGGRSTIHVYPDAPHAFHADYR
                     PSYRKAEAEDGWKRALAHLGAALG"
     gene            complement(1256..1732)
                     /locus_tag="PKN25_04735"
     CDS             complement(1256..1732)
                     /locus_tag="PKN25_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNV90238.1"
                     /translation="MLTVAEAAIPLPLPGVKPGLANIVTLVVLARWGWREAVWVSLLR
                     VLAGSLLLGQFLAPGFFLSLSGALASLVSLGLAVRLPARWFGPVSHSILAAFAHIGAQ
                     LVVARLWLVPHDGVFYLTPVFALAAVVFGLINGLIAARLLRELQAAQRPAGDPMER"
     gene            complement(1788..2177)
                     /locus_tag="PKN25_04740"
     CDS             complement(1788..2177)
                     /locus_tag="PKN25_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HNV90239.1"
                     /translation="MRLELADWRGLLRPGDVLVAIGGLLVCGYALLALWQGGAPDGAV
                     VRAGGKVVARVDLAAERTIEVPGPLGVTVIEIRPGRARVASDPGPRQYCVRQGWLTQA
                     GAVAICAPNEVSLSLTGRAADYDSLNY"
     gene            complement(2174..3073)
                     /gene="rapZ"
                     /locus_tag="PKN25_04745"
     CDS             complement(2174..3073)
                     /gene="rapZ"
                     /locus_tag="PKN25_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNV90240.1"
                     /translation="MQIVLISGLSGSGKSIALNVLEDAGYYVVDNLPAVLLPQLAAHL
                     HGAGWRRVAVAVDMRSGASIAALPQQVEVLRSTVRDLRFIFLEARDDALIARFSETRR
                     RHPMAVDGYTLEEAIQRERDALASVAELGHRIDTSDLHPNTLRAWVKDFIQMAPAEGM
                     TLMFESFGFKYGIPLDADLVFDVRCLPNPHYDPRLRPLTGRDQPVIDFLQGVPEVGRM
                     SQDIHDFVANWLPAYVRDNRSYLTVAIGCTGGQHRSVYIAEWLAERFRGQVEVIVRHR
                     STARRAADRAAGIVDAAGDGNGR"
     gene            complement(3165..4619)
                     /locus_tag="PKN25_04750"
     CDS             complement(3165..4619)
                     /locus_tag="PKN25_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HNV90241.1"
                     /translation="MQATVDWINGWVWSPALVYLCLIVGLYFSIRTRFMQVRHIGEMV
                     RAMFRGKSSAAGVSSFQALTIALSGRVGTGNIAGVATAIGFGGPGAVFWMWMVAFLGA
                     ATAYVESALGQIYKTEHHGLYRGGPAYYIEKGLGWRVYAIVFAVATVFACGVLLPGVQ
                     TNSIAASMENAFGVAPAVTGTIIVILLGLIIFGGVRRIAQVTQVVVPFMALGYILAAA
                     VVVALNVDKVPEVIRLILSSAVGLDAGFGAMIGLAIQWGVKRGVYSNEAGQGTGPHAA
                     AAAEVPHPAAQGLVQAFSVYVDTLFVCSATAFMVLITGTYNVTGPDGAQVFTGLANIS
                     AGTGFTQAAMEQVLPGFGSMFVAVALFFFAFTTVIAYYYIAETNVAYLTRNLRSEALI
                     FVLRVALIGSALYGCVRTSDLAWGLGDIGVGLMAWLNIFAILVLQKPALLALKDYEAQ
                     QAQGRLEPQFDPRALGIVNADLWVKIADRRKREG"
     gene            complement(4715..6913)
                     /gene="ccsA"
                     /locus_tag="PKN25_04755"
     CDS             complement(4715..6913)
                     /gene="ccsA"
                     /locus_tag="PKN25_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNV90242.1"
                     /translation="MSAPRRDLAVWMLVAAATIALACRAELGAFVLAAAAALGAYAAC
                     RSTGRASALALSAALLWSCTLALGWHFAVDHFALRYVWLYSSTALPLHLKIANLWGGD
                     EGTTLLLVACCASLAASGARAGLDSGRRSVAAAIVAAYGAVVLWLAPFAATPADWLAQ
                     SPSQGMNAHLMKPWMLFHAPLVIAAYAWVLALAGPALDALRGGQGKWPARARMHARRA
                     WLLLTAGIGFGMLWAFEDAMYGQVWHWDPVQTAVFVLWCLLGAHLHGINGWGVGRRHW
                     RMMPLAAVLAAAAVPCVMAVTRNPVLASSHRYVGAESWVAHGALGSALLVLALVAAFS
                     GRGAGASLRQGGARSSAGLGLWLAQLCFLGAAAVAVVQLLLASAAAASGAPRPEEYKP
                     FLAMLANLVSGGELEALRAAFEQWDVDGHVLARGLLPSLAGAGLAGGWYFFRRISVGA
                     GRLSLLAALAVTGASAAWGGLMTRHYEGAGILSQQIVALLPQFDAVLAGCAYLALGGG
                     AWTVFMVHRHGLRGIAPALPLLAIHAGVALALSGGLLATALNSYSQHEIVFDGAPSAW
                     TRDRHGYAFRLVDLRVASAGDGGVGGAAGVHALTAIEARAPSGRTFDGETLYRDSRAP
                     PERYDGPLRQICEMLDYRYARHMGRPGYLLDPLVDQGWKHAVQFWVSPAGVVEALAGR
                     AGGRAVFVVVKVFPLISLLWSGLVLLLLGSAWLALAPPGGPARAEPGGGR"
     gene            complement(6915..7928)
                     /locus_tag="PKN25_04760"
     CDS             complement(6915..7928)
                     /locus_tag="PKN25_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNV90243.1"
                     /translation="MRIVAPVSRVAEVAVLAEAGATELYCGLVPPDWRGRFQSISANR
                     RPSGNLASYADLAEAVRSAHAHAAALSLVLNAQHYAAAQIEAAEEIAGRFADLGGDAL
                     IVSDLGLVERLATRFPALRVHVSSVATCRNAAAARLCQALGARRLILPRDVTLAEATA
                     IAEEVPDIEIEAFVLNDGCIFEEGACHTIHLPGQLGGPICLDRYRFRHRRRDGGELSA
                     RLAARLQENDEAYERWLWYRFSCGFSTTPEGLPFGPCGLCALPALRRGRVAAVKIAGR
                     EAPTARKLASVRMVRSVLDRVCAGADAVAVRAFATRLRPSEAHCATGHMCYYPEVLRA
                     EAC"
     gene            complement(7925..8791)
                     /locus_tag="PKN25_04765"
     CDS             complement(7925..8791)
                     /locus_tag="PKN25_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90244.1"
                     /translation="MSFSELAVTATHAVDLAWAAGGPAERERHWLRIHGDAALAAWLA
                     GLQPTHLHFGSEFCEHLLPAERVLREALRRVEEAGLRFVLLTPVASPDVLARLHGLLP
                     MLPAGTEVVANDWGVAHELHTRFPALLPVAGRVLCRMTKDPRLHPGWAGRCGHGLAAP
                     TMRALFERLGILRLELDMPVFADDGALEGLPLPAGVHLPCVYVAKGRMCRAGGLSMKG
                     PERFAVGRRCRKECLRLAAEASRPGRDDACRTIQLGNTLFSRHSDAMAQALRRAADAG
                     RIARLVVAGEPL"
     gene            complement(8874..10376)
                     /locus_tag="PKN25_04770"
     CDS             complement(8874..10376)
                     /locus_tag="PKN25_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNV90245.1"
                     /translation="MDRYKGELLSDDTRRFLAQPKRMAIGGEWVEALGGGMLEVVDPA
                     SGQVFDRVPAGEATDIDRAVAAARRAFEQGDWPRMRPVDRERLLLRLAELVEAHAQEL
                     AEIEALDNGKPVTMARAVDVALVVDFLRYMAGWATKIEGSTMEVSVPLVRDREFFGYT
                     RREPVGVVGAIIPWNFPLLMVAWKAGPALASGCTMVLKPAEETPLSALRFAELVQQAG
                     YPAGVFNVVTGHGHSAGAALAAHKGVDKVAFTGSTEIGKLVGKAALDNMTRVSLELGG
                     KSPVIVLDDADPAVAAAGAAQAIFFNQGQVCCAGSRLYVHKSRFERVVEGLSGIAADM
                     KLGAGIEPSTQIGPLVSAVQQQRVLGYIRSGFEEGARALAGGAAGEGEGYFVKPTVLV
                     DTRDDMRVVREEIFGPVVVAMPYDDLDEVARRANDTPYGLGASIWSNDLSRVHRLVPK
                     IKAGTVWVNCHNILDASMPFGGYKQSGIGREMGRAVLDLYTEGKSVIMAL"
     gene            complement(10430..11746)
                     /locus_tag="PKN25_04775"
     CDS             complement(10430..11746)
                     /locus_tag="PKN25_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNV90246.1"
                     /translation="MRMRITTAVLLAGLAALGTARAETLHERDGTTLSANIDGMAGAF
                     STREDYLGEGGRQWREGFIHGVLAGTTRAGGGKLYGGLGAIAQGTWGDGDAGGYTDGR
                     ERDVDLEDAYVGWRSAGGAFDVSFGRQQFVLGDGFLIASDRINLGKGLDALGVDVDRG
                     GAYYLAAKKSFRNTAIVRIDPEGPWRGDAFWLQSGNPYQQDTELGGANLEYVSETHGT
                     LGLSWMKVLDVDQGAGLGIFDRRDGMRIASVRGQGSLGVENLFLAFEYVDQRGGSAAV
                     KNDADAWHVEGGWTFADLPWRPTLDYRHARFSADKSATARNEAFDPLFFGLSRGLGTW
                     FQGEVASNYAGPANSGNRVDRVELSLAPREDLSIGVQYWKFAPEGEAADLAGREFDLF
                     AFWQIDKHFTFVPLIGLYEPRGADVKALQGNDRRNVYLQAVLIFNY"
     gene            complement(11856..12992)
                     /gene="peaD"
                     /locus_tag="PKN25_04780"
     CDS             complement(11856..12992)
                     /gene="peaD"
                     /locus_tag="PKN25_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009310 - amine catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     beta"
                     /protein_id="HNV90247.1"
                     /translation="MKKTMIGGALRRSAAALACLAAGVLPAAAANEARPLEPGSEYLM
                     VANYPNNLNVVDLASESVYKTCPLPDAFGPGTTQIAPDRRTAYILNNRFGTIYGIDLD
                     TCRLTFRAEMSQGDNERAKSIASFTVSADGKEVYAVQNPTTIHRDHYRVGAPRFAVYD
                     TRAGLEAKPVRVFPAPRQANIMQAGEDGSVYLAGPNLYKVDVNTGAMTVALPIRDWQR
                     STHAPLDVLYIWPVQTPTRDFTILYTTAKYQDDKQDMATAEYQYGYLNVNLKSGETEA
                     REFGPLTEIYFTGLRSPKDRNIVYGLLHRLTKYDIAQQKLVQAAELDHTYYTVLSNQA
                     GSRLYLTGTFNDISIHDADTLAKVGQVKLPGGDMSLGTGQVFVR"
     gene            complement(13003..13329)
                     /gene="qhpC"
                     /locus_tag="PKN25_04785"
     CDS             complement(13003..13329)
                     /gene="qhpC"
                     /locus_tag="PKN25_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     gamma"
                     /protein_id="HNV90248.1"
                     /translation="MKHLKAINNKARLLDKAVAEDRVEEVVAMSAIAGCAATVDPGWE
                     VDAFGGVSSLCQPMESDLYGCADPCWWPAQVPDVMNTYPDWNKDATNSREDWRNLGSV
                     FPGDKL"
     gene            complement(13326..14756)
                     /gene="peaB"
                     /locus_tag="PKN25_04790"
     CDS             complement(13326..14756)
                     /gene="peaB"
                     /locus_tag="PKN25_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase maturation
                     protein"
                     /protein_id="HNV90249.1"
                     /translation="MGAVLNLVQDNLHELRIDETRMLFHIPSSSLFALDPLSAALIDR
                     IRRQSLTVEALIDGLRTEFDADEVAEAIRELVALELIDDGRPASAGTLAHTFERFPLT
                     TVVLNVNTGCNLSCTYCYKEDLDKPSAGRKMAFGTARDAIEMMLRESPDEPRYNVVFF
                     GGEPLSNLPLIKDVVAYCEARFAELGKQVDFVMTTNATLLADDTIDWLDAHRFGLSIS
                     IDGPKAIHDRNRLTVGGQGTYETVRRKAERLLARYHARPVGARVTLTHGTTEVERIWD
                     HLFNELGFAEVGFAPVTSGDISTFNLTGAELVEVFAGLKRLGARYLEAALEGRNIGFS
                     NMHQLITDLHEGHKKALPCGAGLKMLAVDHKGELNLCHRFTGSTLPTFGDVKNGIQRA
                     QLGDFLSQRLDRTDTGCASCRIRNLCSGGCYHESYARYGDPAHPTYHYCDLMRDWVDF
                     GIEVYSRIMAGNPAFIEQHISTRTAS"
     gene            complement(14825..16408)
                     /gene="peaA"
                     /locus_tag="PKN25_04795"
     CDS             complement(14825..16408)
                     /gene="peaA"
                     /locus_tag="PKN25_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009310 - amine catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     alpha"
                     /protein_id="HNV90250.1"
                     /translation="MKIRTLATAMGSMAALGVVLAAPPAHASAEALIRAKCLPCHTET
                     EDGISRISHQRKTPEGWLMSVARMQIVHGLKITDEERRTVVKHLADRQGLAPSETTGV
                     RYAMERRLNTVEAFESEKFTQMCARCHSGARVMLQRRPAAEWEHLVHFHLGQFPTTEY
                     QALGRDRDWFGIALKEMVPELASKLPLQSEAWKQWQARAPQTVKGEWSLSGHLTGRGG
                     FSGVMKVTAGEGRDRYTLAFDGRWDDGRPMQGRGRALIYTGYEWRGDLVVDGTPMRQV
                     FAVEDGMMRGRMFLRDQDEVGADVVASLQQAGNSRVLAVHPAYLKAGEGAELRIVGSG
                     LAGEVTLPQGVKLVGTVKRSATEVVVRVQSDAAARGVHAVAVGKASGGSLALYDSIAA
                     VKVSPAFAVARIGGNGTPTAKVEARFDAEAWAAGADGKAGTEDDFRIGVVPAKWSVEP
                     FDEAAVREQDVEFAGLMDAASGVFVPGDAGPNPVRRMSASNVGNLKVVAEVAQGGERL
                     RGEGQVIVAPPRWNNPPLP"
     gene            16929..17912
                     /locus_tag="PKN25_04800"
     CDS             16929..17912
                     /locus_tag="PKN25_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV90251.1"
                     /translation="MSPAAQPSGNAPYSYTLRHTLDADEHAACLTNWHQRYDQLTAGA
                     FDGVFEEFCFGRVQLFREGLNQSVHQAGGAWPGSRTFAVPVAIEGTGWFGGEMYDAHS
                     MLTLGGDDELDFRTPRRLEILACTADSAALHDYAHQVDHRNLEAELAGRKLAPTTPAG
                     IAALGQLLATMTASLRATPELLLHPQMRKAMEQALFATLLDTLSCGGGRAGAPSCRAR
                     QQVVARARAYMEAHIDEPITVADLCIELGISRRTLQYSFQDVLDLNPVKFLRAIRLNA
                     VRRALKAADPNGRGTVADIAARWGFWHLSHFSAEYKTMFGELPSDTLRRAG"
     gene            complement(18014..19357)
                     /locus_tag="PKN25_04805"
     CDS             complement(18014..19357)
                     /locus_tag="PKN25_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV90252.1"
                     /translation="MAADGIVILGAGPAGAAVAIGLARMGEPVVLVGEPRRFAAVEGV
                     SARVIDALRGLGLQRALTAFAPPSPRRAVWNGTYTEANVESLVDRQRFDQGLLEDLER
                     LGVPVVRGRVTALHPAPGGHELEIDTDAGPRRMAAGFLVEARGRAAPGGGAPRVRGVE
                     TVSLLQYWQGPAGEAGSTVLSVEDGWMWMAALADGRRYLQLTVDVASADLPPKRALGD
                     YCSARFHAVEAAAPFVRDAQPVGEPHARTSTAVLNESVAGDDWIRVGDAAMAVDPLSG
                     NGIFQALSSALQAPAVVATLRHDRGRTALAQHFHTRRIEHLFHRFARIGRDFYAQEAR
                     WPQAPFWAARRGWPDALPLHAKVRPETVRVARGPVVCAGRIVEQDVVVTPDQPLGVWH
                     LDGLAVAPMLAALRREGGDALEKAAGEALLCARFGLERARAAALLAWMRAQNWLD"
     gene            complement(19347..20030)
                     /locus_tag="PKN25_04810"
     CDS             complement(19347..20030)
                     /locus_tag="PKN25_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNV90253.1"
                     /translation="MTVRIAVVDSGCTPAQALQVRDAVALVLEDRAVRQAPATADALG
                     HGGRVAGILLHCAPQAELLVVQVFRERLTTTAAQVAAAIDWAVANGAQLVNLSLGLRE
                     PRPVLAEACARALAAGTVLCAAAPARGQTVYPAGFPGVLRVTGDARCAPGELAAIGSA
                     EIDFGAHVRPLDGSLAGAGASMACAWFTGLAARHFARGGDADALRAWLVEQARHHGVD
                     DPRRRQHGG"
     gene            complement(20027..21763)
                     /locus_tag="PKN25_04815"
     CDS             complement(20027..21763)
                     /locus_tag="PKN25_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90254.1"
                     /translation="MLAGLIHAERPDELRRGLRWLYGFVRPQRRRIAGLLLLSCAATL
                     LVLLQPWLTKLLIDEGLLARDYRTLLMVAGAMIAVGIVGTVLAGVNRQLHTRLSGSIL
                     FALRSDLYRHLQTLSPAFYGRQRMGDLLSRLDGDVAEIQRFAVDSLFAAVSSVIGLLG
                     AVALMLSLSWKLSLLVLVLVPLETLWLRRMRRKVEARTRTVRERAADVSSFLVETLPA
                     MKFIQASRREADERSRLDDLGARYLDDLLRLQRTEFLTHAIPSTLTSATRAAAFMIGG
                     WWVIEGSWQLGALIAFSTYLGMATGPVNSLLGLYVAIQRMSVSLRRVSELREAPADVV
                     DGAHPGAAQRAVPAWQGRIEFDAVVFHHQGRAEAVLDGARLTVPAGSKVALSGASGVG
                     KSTLIDLLHRHYDPQAGTIRLDGADLRALPLGALRRAVAVVSQDIVLFRGTLAENIRY
                     AAPQADEGAVRAAARRAHLDALIASLPLGLETALGERGQQLSGGQRQRIAIARALLQE
                     PCVLVLDEATSAVDEATEAAIIAEVDALFAGRTRILISHRAATLAGCDLRVHLERGRL
                     EVLPPAAVTVRS"
     gene            complement(21858..22778)
                     /locus_tag="PKN25_04820"
     CDS             complement(21858..22778)
                     /locus_tag="PKN25_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV90255.1"
                     /translation="MSARPHLPASLQVFERGWLSANNVLLFDGDEATLIDSGYVSHAD
                     QTVKLVRAALDGRSLGRLINTHSHSDHIGGNASVQRAFGCSITVPEGMARAVEEWDEE
                     ALLLSVGAQAGERFRHDATLAAGARFVAGELEWQAVAAPGHDMDALAYYNPDRRILIS
                     GDALWRDGFGILFAEVMGQGDALGEARRTLEAIGRLAVDAVIPGHGAPFAEFDDALER
                     AFARLRAFEDDGARMARNAIRACITFKLLEVRSMALVELPRHLDETPLYRTANARFLG
                     LDADALAAWLVKELERAGVARREHGMLVAS"
     gene            complement(22775..22996)
                     /locus_tag="PKN25_04825"
     CDS             complement(22775..22996)
                     /locus_tag="PKN25_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNV90256.1"
                     /translation="MSGDALCVGVCMIDWDSGVCLGCGRTADEINGVPTPAPGAQVAG
                     QPDAAASPAQAAGVPLPPNVAARVGEGSD"
     gene            complement(22993..23193)
                     /locus_tag="PKN25_04830"
     CDS             complement(22993..23193)
                     /locus_tag="PKN25_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNV90257.1"
                     /translation="MSVPSPCINLCRMSPDTGWCEGCQRTIEEITRWSRSTDDDRRAI
                     LAAVAERRTRLAAASPAVDLRT"
     gene            complement(23355..24290)
                     /locus_tag="PKN25_04835"
     CDS             complement(23355..24290)
                     /locus_tag="PKN25_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNV90258.1"
                     /translation="MANSILPAAVRIVEMGPRDGLQNEKQLVSTDTKLELVRRLERAG
                     LKAIETTSFVSPKWVPQMGDNAELLPRVLAAAAPGVAYPVLTPNLKGFEAALAAGARE
                     VAVFGAASESFSQKNINCSIAKSLERFRPVTEAAHAAGVRVRGYVSCVVGCPYEGAVA
                     PQAVADVAATLLEMGCYEVSLGDTIGVGTPAGIRRMLEAVAKRVPVDKLAGHYHDTYG
                     MAVANVFASLEMGVAVFDASVGGLGGCPYAAGASGNVATEDVVWLLNGLGIDTGIDLD
                     ALVDTAAWISAQLGREPASRVARAVLAKRAKTACA"
     gene            complement(24400..26091)
                     /locus_tag="PKN25_04840"
     CDS             complement(24400..26091)
                     /locus_tag="PKN25_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV90259.1"
                     /translation="MSSTASYVHGASAKPLIGQTIGRFFDEACARHAGREALVVRHQD
                     VRLTYAQLKSKVDALACGLMRLGLEPGERIGIWSQNNMEWALTQFASAKAGLVLVNIN
                     PAYRRSELEYALEKVGCRALILSPAFKTSNYLEMIADLAPELGHCEPGLLRSHRLPSL
                     EMVIRMGAEKSPGMMSFDDLLRAPSREELTALAVLSEKLQFDDPINIQFTSGTTGHPK
                     GATLSHHNILNNGFFVGEAIKLMPGDRLCIPVPLYHCFGMVMGNLGCLTHGATMVYPG
                     EAFEPLAVLETVAQEKCTGLYGVPTMFIAALDHPRFAEFDLSSLRTGIMAGSPCPIEV
                     MKRVIGKMNMSEVTIAYGMTETSPVSFQSGTDDPIDRRVSTVGRIQPHLEVKIVDNEG
                     RIVPRGQSGELCTRGYSVMLGYWDDEARTKEAIDAGGWMHTGDLAVIDDEGYCNIVGR
                     IKDMVIRGGENIYPREIEEFLYAHPKVLDVQVVGIPDQKFGEELCAWIIPREGERLTE
                     DEVREYCKGQIAHYKIPRYIRFVDSFPMTVTGKIQKFLIRQRMKEELGLDEAKTA"
     gene            complement(26554..28557)
                     /locus_tag="PKN25_04845"
     CDS             complement(26554..28557)
                     /locus_tag="PKN25_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HNV90260.1"
                     /translation="MFDKILIANRGEIACRVIKTARRMGIKTVAVYSEADANARHVRL
                     ADEAVLIGPAAARESYLVIDKIIAAAKQTGAQAIHPGYGFLSENEDFCHACEREGIVF
                     IGPPVSAIRAMGSKSEAKKLMEAAGVPLTPGYHGDDQDPAWLHQQADAIGYPVLIKAA
                     AGGGGKGMRLVDRSEDFIDLLASCKREAISSFGDDHVLVEKYITKPRHIEIQVFGDTH
                     GNVVYLFERDCSVQRRHQKVLEEAPAPGMTLERRAAMGKAAVDAAKAVGYVGAGTVEF
                     IANQDGSFYFMEMNTRLQVEHPVTEMITGLDLVEWQLRVASGEKLPLAQEQLQIRGHA
                     LEARIYAEDPAKGFLPSTGKLVHLAPPAESLHVRVDTGVEEGDEISPHYDPMIAKLIV
                     WDINRDRALARMLQALADYRVVGVANNIEFLSRLTACPAFAGADLDTGLIEREKAYLF
                     PADEAVPAEVLQIAALAELLRDAAFADRRAARGADPASPWHARDGWRMNATACRSLLF
                     RHGESERAVEVAYLPGAWRLTVGSSSVVARGELNPRGLLRVELDGTRMDATVIAAAGR
                     RHVFARGRAWQLAAVDPLHHGGEGGGAEGGLMAPMPGKVIALVAAEGAKVEKGAPLLI
                     LEAMKMEHTITAPAAGTVKAFRFGVGDQVGDGAELVEFEAAAV"
     gene            complement(28601..29392)
                     /locus_tag="PKN25_04850"
     CDS             complement(28601..29392)
                     /locus_tag="PKN25_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNV90261.1"
                     /translation="MSYETLEIVRAAGVATIWMNRPDVHNAFNAQLIADLTTACIELD
                     ADEAVRAVVLAGRGKSFSAGADLNWMKAAGEASEAENFADAMRLAGMLRTLAEMNKPT
                     IARVHGAALGGGMGLASACDICIAGDKAVFATSEVKFGIIPSAISPYVIRAIGERQAT
                     RYFQTAERIGAARAAELGLAHEAVASEELDAKVKEVVEALLQGGPKSQAAAKDLIRAV
                     ANRPLSDALVEDTARRIASLRATPEAKEGLAAFLDKRPAAWIAQA"
     gene            complement(29402..>30474)
                     /locus_tag="PKN25_04855"
     CDS             complement(29402..>30474)
                     /locus_tag="PKN25_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNV90262.1"
                     /translation="QIAVVMGSCTAGGAYVPAMSDEAVIVKNQGTIFLGGPPLVKAAT
                     GEVVSAEDLGGGDVHTRISGVADYLAENDSHALHIARRIVSNLNQRKEVELALADSEE
                     PAYDPEEIYGIIPADPRKPYDVREVIARVVDGSRFDEFKPRYGTTLVTGFARLYGYPV
                     GIVANNGILFGESAQKGAHFVELCGQRGIPLLFLQNITGFMVGRKYENGGIAKDGAKM
                     VTAVATVQVPKITMIMGGSFGAGNYGMCGRAYSPRFLWMWPNARVSVMGGEQAASVLS
                     TVRRDGIEARGGTWSKEDEEAFKAPIREQYEVQGHPYYATARLWDDGVIDPAQSRRVL
                     GLSLSATLNAPIPQTKFGVFRM"
BASE COUNT         4665 a        11086 c        10365 g         4358 t
ORIGIN      
        1 ccccacgcgg cccctcgggg gccgcgcgtc gtttacgcgc cccctttccc gctccacgcg
       61 cggaacgacc aggcacaagg cgctgtctct ccggggcatg accactaccc cccagaggag
      121 accgccacga tgcaagccac ccccagccgt gcgcaacacg ccaccgaaga cgagcgcggc
      181 cagttcgaca gcctgctccc cgccgcccgc ctcgaccgcc gcggctttct cgcaggcatc
      241 gccgccaccg gcttcgcgct cgccgtgcag cctgtccatg ccagcaccgt gatcagcacg
      301 agcggcaccg gcctcgccac gggcacggcc gccatcgccg tcgagggcgg cgagttgccg
      361 ctctactacg cgcggcccgc cgcaggcgac aggttgccgg tcgtgctcgt ggtgcaggag
      421 atcttcggcg tccacgaaca catccgcgac gtctgccgcc gcctcgcaca cgcgggctac
      481 ctcgcgatcg cgcctgaact cttcttccgc caaggcgatc ccaccaccca gcccgacatc
      541 cccgcgatcc tgcagaacat cgtcgccaag gtgcccgacg cccaggtcat gggcgacctc
      601 gacgcctgcg ccgcatggtc ggcgagccag ggcggcgacc ccgcccgcct cgcgatcacc
      661 ggcttctgct ggggcgggcg catcacctgg ctgtacgccg cgcacaagcc atcggtaaaa
      721 gccggcgtgg cgtggtacgg acgcctgagc ggcgccattt cggagttcac cccgcagcac
      781 ccgctcgacg tcgtcggcag gctgcacgcg ccggtgctgg ggctctacgg cggccaggac
      841 cagggcatcc cgctcgccga cgtggagaag atgcaggcgg cgctctccgc tgcgggcggc
      901 cgcagcacca tccacgtcta tcccgacgca ccccacgcct tccacgccga ctaccggccg
      961 agctaccgca aggccgaggc cgaggacggc tggaagcgcg cgctcgcgca ccttggcgcg
     1021 gcgctcggct gagcactcgc cgccgcacac tgcgccggcg ggtgccgcac ggcatgcgac
     1081 atcccgacgg gtgccgcatg caccaggccg gagcccgcga cccagccgaa caccaggccg
     1141 ctgacgaccg cgagcaccgt gccaagccac ctccaccacc gtccgtgcat gccaccaccc
     1201 tccgctgcgc cggcgctgcc ttgccggcat cggctggttg gacggcagga gcgggtcagc
     1261 gttccatcgg atcgcccgcg ggccgctgcg cagcctgcag ctcgcgcagc aggcgcgcgg
     1321 cgatcagccc gttgatcagg ccgaacacca ccgccgcgag cgcgaacacc ggggtgaggt
     1381 agaacacccc gtcgtgcggc accagccaca ggcgcgcgac caccagctgt gcgccgatgt
     1441 gggcgaatgc cgcaaggatg ctgtggctca ccgggccgaa ccagcgtgcc ggcaggcgca
     1501 ccgccagacc gagcgacacc aggctggcga gcgcgcccga aaggctcagg aagaagccgg
     1561 gggcgaggaa ctggccgagc agcaggctgc cggcgagcac gcgcagcagc gacacccaca
     1621 ccgcctcgcg ccagccccag cgtgccagca ccaccagggt cacgatgttg gccagccccg
     1681 gcttgacccc gggcagcggc agcgggatcg ccgcctcggc cacggtcagc acgatcgccg
     1741 ccgccgcatg gcgggcgatg cggcggtcct cggcggtggg agtcagttca atagttgagg
     1801 gagtcataat ccgcagcccg cccggtgagg ctcaggctca cctcgttggg cgcgcagatc
     1861 gccaccgcgc cggcctgggt gagccagccc tgacgcacgc aatactggcg cggcccggga
     1921 tcggaagcga cacgcgcgcg cccgggccgg atctcgatca ccgtgacgcc aagcgggccg
     1981 ggcacctcga tcgtgcgctc ggctgccagg tccacccgcg ccaccacctt gccgccggca
     2041 cgaaccaccg cgccgtccgg cgcaccgcct tgccacagcg cgagcagcgc atagccgcac
     2101 acgagcagcc cgccgatcgc caccaggaca tcgccgggcc gcagcagccc gcgccagtcc
     2161 gcaagctcca gcctcaccgc ccgttcccgt ctccggccgc atccacgatg ccggcagcgc
     2221 ggtccgccgc ccggcgcgcc gtcgagcggt ggcgcacgat gacctcgacc tggccgcgga
     2281 aacgctcggc cagccactcg gcgatgtaca ccgagcgatg ctggccgccg gtacagccga
     2341 tcgccacggt caggtagctg cggttgtcgc gcacgtaggc gggcagccag ttggcgacga
     2401 aatcgtggat gtcctgcgac atccgcccga cctcgggcac gccctggagg aagtcgatga
     2461 ccggctggtc gcgcccggtg agcggacgca ggcgcgggtc gtagtgcgga ttgggcaggc
     2521 agcgcacatc gaagacgagg tcggcatcga gcgggatgcc gtacttgaag ccgaaggact
     2581 cgaacatcag cgtcatgccc tcggccggcg ccatctggat gaagtccttc acccacgcgc
     2641 gcagcgtgtt cgggtgcagg tcgctggtgt cgatgcggtg accgagctcg gccacgctgg
     2701 cgagcgcgtc gcgttcgcgc tggatcgctt cttcgagcgt gtagccgtcc accgccatcg
     2761 gatggcgccg gcgcgtctcg gagaagcgcg cgatcagcgc gtcgtcgcgc gcctcgagga
     2821 agatgaagcg caggtcgcgc accgtcgagc gcaagacctc gacctgctgc ggcagggccg
     2881 cgatgctcgc gcccgagcgc atgtccaccg caaccgccac ccgccgccag cccgcaccgt
     2941 gcaggtgcgc cgccagctgt ggcagcagca ccgccggcag gttgtcgacc acgtagtagc
     3001 cggcatcctc gagcacgttg agcgcgatgc tcttgcccga acccgacaag ccgctgatta
     3061 gaacgatctg cattgccctt ccggatgaaa aagcccgccc acactatagc ccagtgtcgg
     3121 cgggcgggtc ctgcacgcgg ggacggcggg cttcccccgc ccgatcagcc ttcgcgcttc
     3181 ctgcgatcgg cgatcttcac ccacaggtcg gcattcacga tgccgagcgc gcgcgggtcg
     3241 aactggggct cgaggcgtcc ctgggcctgc tgcgcctcgt agtccttgag cgccagcagc
     3301 gccggcttct gcagcaccag gatggcaaag atgttgagcc acgccatcag gccgacgccg
     3361 atgtcgccca ggccccaggc caggtcgctg gtgcgcacgc agccatacag ggccgaaccg
     3421 atcagcgcca cgcgcagcac gaagatcaag gcctccgagc gcagattgcg cgtcagatag
     3481 gcgacgtttg tctcggcgat gtagtagtag gcgatcaccg tggtgaaggc gaagaagaac
     3541 agcgccaccg ccacgaacat cgagccgaag cccggcagca cctgctccat cgccgcctgg
     3601 gtgaagccgg tgcccgccga gatgttggcc agcccggtga acacctgcgc gccgtccggt
     3661 ccggtgacgt tgtaggtgcc ggtgatcagc accatgaagg cggtcgccga gcacacgaag
     3721 agggtatcca catacaccga gaaggcctgc acgaggcctt gcgcagccgg gtgcggcact
     3781 tcggcggcag cggcggcgtg cgggccggtg ccctggccgg cttcgttgga atacacgccg
     3841 cgcttgacac cccactggat ggccaggccg atcatcgcgc cgaagcccgc gtcgagaccg
     3901 acggccgagg acaggatcag gcggatgacc tcgggcacct tgtcgacgtt gagcgccacc
     3961 accacggccg cggcgaggat gtagccgagc gccatgaagg gcaccaccac ctgggtgacc
     4021 tgggcgatgc ggcgcacgcc accgaagatg atcaggccga gcaggatcac gatgatggtg
     4081 ccggtgaccg ccggcgccac gccgaaggcg ttttccatgc tggccgcgat gctgttggtc
     4141 tgcacgccgg gcagcagcac gccgcacgcg aacacggtgg cgaccgcgaa cacgatcgca
     4201 tagacgcgcc agcccaggcc cttctcgatg taatacgccg gaccgccacg gtagaggccg
     4261 tggtgttcgg tcttgtagat ctgcccgagt gccgactcga cgtaagccgt ggcggcgccg
     4321 aggaaggcca ccatccacat ccagaacacc gcgcccggac cgccgaagcc gatcgcggtg
     4381 gcgacgccgg cgatattgcc ggtacccacc cgaccggaga gcgcgatggt gagcgcctgg
     4441 aaggacgaca cgcccgccgc ggagctcttg ccgcggaaca tcgcccgcac catctcgccg
     4501 atgtggcgca cctgcatgaa acgggtgcgg atggaaaagt acaggcccac gatcaggcac
     4561 aggtaaacga gggcggggct ccagacccag ccgttgatcc agtcgacggt cgcttgcatt
     4621 cttgtgtttt cccgagaaac gagccgcgat tgtatctgct tgttgcgagc ggatgagcgg
     4681 cagcatcgtt gggaataacc cggacactgc ccattcaacg ccctccgccg ggctccgccc
     4741 gagccggccc gcccgggggc gccagagcca gccacgcact gcccagcagc aggagcacga
     4801 gcccgctcca cagcagggag atcaggggga agaccttcac cacgacgaac accgcccggc
     4861 ctccagcgcg tcccgcaagc gcctccacca cacccgcggg ggaaacccag aactgcaccg
     4921 catgcttcca gccctgatcg accagggggt cgagcagata accgggcctg cccatgtggc
     4981 gggcgtaacg gtagtccagc atctcgcaga tctgccgcag cgggccatcg tagcgctccg
     5041 ggggcgcgcg cgagtcccgg tagagcgtct ctccgtcgaa ggtcctgccc gagggagcgc
     5101 gcgcctcgat cgcggtcagc gcatgcacgc cggcggcgcc accgacgcct ccatcgcctg
     5161 cgctcgcgac cctcagatcg accagcctga aggcgtagcc gtggcgatcg cgcgtccacg
     5221 cgctcggcgc gccgtcgaag acgatctcat gctgcgagta gctgttcagc gcggttgcca
     5281 gcaggccgcc ggaaagggcc agcgcgaccc ccgcgtggat ggccagcagt ggcaaggccg
     5341 gcgcaatccc gcgcagccca tgccgatgca ccatgaatac cgtccacgcg ccaccgccga
     5401 gcgcaagata cgcacagccc gcgagcacgg cgtcgaactg cggcaggagc gccacgatct
     5461 gctgggagag gatccccgca ccctcgtagt gccgcgtcat caacccgccc caggctgcgc
     5521 tcgcccccgt cacagccagc gccgccagca gcgacaggcg gcccgccccg accgagatgc
     5581 ggcggaagaa gtaccagcct ccagccagtc cggccccggc cagcgacggc aagaggccgc
     5641 gtgcgagcac atggccgtcc acgtcccact gctcgaaggc cgcgcgcagc gcctccagtt
     5701 cgcctccgct cacgaggttg gcaagcatcg cgaggaaggg cttgtattcc tccggacgtg
     5761 gcgcgccgct cgccgccgca gcggacgcga gcagcaactg caccacggcg acagcggctg
     5821 cgcccaggaa gcagagctgt gccagccaca ggcccaggcc agcggacgaa cgcgcacccc
     5881 cctgccgcag gctcgcgccg gcaccgcgac cggaaaacgc tgcgacgagg gcgagcacca
     5941 gcaacgcgct gccgagcgca ccatgtgcca cccaggactc ggcccccacg tagcggtgcg
     6001 agctcgccag caccggattg cgcgtcaccg ccatcacgca gggcaccgcc gcagcggcca
     6061 gcacggcggc cagcggcatc atgcgccagt gcctgcggcc cacgccccag ccgttgatcc
     6121 cgtgcaggtg cgcccccagc aggcaccaca ggacgaacac cgcggtctgg accggatccc
     6181 agtgccacac ctggccatac atcgcatcct cgaaggccca cagcatgccg aatccgatcc
     6241 ctgcggtgag cagcagccag gccctccgcg catgcatgcg cgcgcgcgcc ggccacttgc
     6301 cctgcccgcc gcgcagggcg tcgagcgcag gcccggcgag tgccagcacc caggcgtagg
     6361 cggcaatcac cagcggggcg tggaacagca tccagggttt catcaggtgc gcgttcatgc
     6421 cctgcgaggg cgactgtgcc agccagtcgg cgggcgttgc ggcgaaggga gccaaccaca
     6481 ggacgaccgc gccataggcg gcgacgatcg cagccgccac cgagcgacgc ccgctgtcca
     6541 gtccggcgcg ggcgccactc gccgccaggc tcgcgcagca ggccaccagc agcagcgtgg
     6601 tgccttcatc gccgccccac aggttggcga tcttcaggtg cagcggcagc gctgtgctgc
     6661 tgtacagcca gacgtagcgc agcgcgaaat ggtcgactgc gaagtgccag cccagggcga
     6721 gcgtgcaact ccaaagcagc gccgccgaca gggcgagcgc actcgcgcgc cccgtcgatc
     6781 tgcacgccgc atacgccccc agcgccgctg cggcggcgag gacgaacgcc cccagctccg
     6841 cccggcatgc caaggcgatg gtcgcggcgg cgaccagcat ccagaccgcc aggtcacgac
     6901 gtggcgcgct cacttcagca tgcctccgcc cgcagcactt cggggtagta gcacatgtgg
     6961 ccggtggcgc aatgcgcctc ggacggccgc aggcgggtgg cgaacgcgcg caccgcgacg
     7021 gcatccgccc ctgcgcagac ccggtcgagc acggacctca ccatgcgcac gctcgccagt
     7081 ttgcgcgccg tcggcgcctc gcgcccggcg atcttgaccg cggccacccg tccgcgcctg
     7141 agcgcgggca gcgcgcacag cccgcatggt ccgaagggca agccttcggg cgtggtgctg
     7201 aagccgcagg aaaagcggta ccacagccag cgctcgtagg cctcgtcgtt ctcctgcaag
     7261 cgtgcggcga ggcgtgccga cagctccccg ccgtcgcgcc gacgatggcg gaagcggtag
     7321 cggtcgaggc agatcggccc gcccagttgc cccgggaggt ggatcgtgtg gcaggcgccc
     7381 tcctcgaaga tgcagccatc attgagcacg aaggcttcga tctcgatgtc cggcacctct
     7441 tcggcaatgg ccgtggcttc ggcgagggtc acgtcgcgcg gcaggatcag ccggcgcgca
     7501 ccgagggctt ggcacagccg cgccgccgcc gcgttgcggc aggtcgccac cgaactgacg
     7561 tgcacgcgca gcgccgggaa acgcgtcgcc aggcgctcca ccaggcccag gtcgctgacg
     7621 atcagggcat cgccaccgag gtccgcaaag cgcccggcga tctcttccgc ggcctcgatc
     7681 tgcgctgcag cgtagtgctg ggcattgagc accagcgaca gcgcggcagc gtgggcatgc
     7741 gcgctccgca ccgcctccgc caggtctgca tagctcgcaa gattgcccga cggccggcga
     7801 ttggcgctga tcgactggaa gcgtccgcgc cagtcgggcg gcaccaggcc gcaatacagc
     7861 tcggtggcgc cggcctcggc gagcactgca acctcggcga cgcgcgaaac aggcgcgacg
     7921 attctcacag cggttctccg gcaacgacca ggcgggcgat ccgccctgcg tcggcggccc
     7981 gccgcaaggc ctgcgccatc gcgtccgaat ggcggctgaa caaggtgttg ccgagctgga
     8041 tcgtccggca ggcatcgtcc cgtcccgggc gtgaggcctc ggcggcaagg cgcaggcact
     8101 ccttgcggca gcggcgtccg acggcgaagc gctccggccc cttcatggaa agcccacccg
     8161 cccggcacat gcggcccttg gccacataga cgcagggcag atgcacgccg gcggggagcg
     8221 gcagcccctc gagcgcccca tcgtcggcaa agaccggcat gtcgagctcg agccggagga
     8281 tgccgaggcg ctcgaacagt gcgcgcatcg tcggcgcggc caggccgtga ccgcagcgcc
     8341 ccgcccagcc gggatgcagg cgcggatcct tggtcatgcg gcacagcacg cggcccgcca
     8401 ccggaagcaa ggccggaaag cgcgtgtgca actcgtgcgc cacaccccag tcgttcgcca
     8461 ccacttccgt cccggcgggg agcatgggca gcagcccgtg cagtcgggcc aacacatcgg
     8521 gcgaggcgac cggcgtgagc aggacgaagc gcaggcccgc ctcctccacc cgccgcagcg
     8581 cctcgcgcag cacccgctcc gcgggcagca ggtgctcgca gaactcgctg ccgaaatgaa
     8641 gatgcgtcgg ctgcagcccc gcgagccagg cggccagggc agcatcgcca tggatgcgca
     8701 gccagtggcg ttcgcgctcc gcgggccccc ccgcggccca cgccagatcg actgcgtgcg
     8761 ttgccgtcac cgccaactcg gaaaaactca tcacgctttc tccccgcggc ctcgggtgcc
     8821 gcgacaaggg atcgaaaggg catggagcgc cgcagccgcg cgacgctccc cccttacagg
     8881 gccatgatca ccgacttgcc ctcggtgtac aggtcgagca ccgcgcgccc catctcgcgg
     8941 ccgatgccgg actgcttgta gccgccgaag ggcatcgagg cgtcgaggat gttgtggcag
     9001 ttgacccaca ccgtaccggc cttgatcttc ggcaccagcc ggtgcacccg cgacaggtcg
     9061 ttggaccaga tgctggcgcc gaggccgtat ggggtgtcgt tggcgcggcg ggcgacctcg
     9121 tcgagatcgt cgtagggcat cgccaccacc accgggccga agatctcctc gcgcaccacg
     9181 cgcatgtcgt cgcgggtatc gaccagcacc gtcggcttga cgaagtagcc ctcgccctcg
     9241 cccgctgcgc cgcccgccag cgcgcgcgcg ccctcctcga agccgctgcg gatgtagccc
     9301 agcacgcgct gctgctggac ggccgacacc agcgggccga tctgcgtcga gggctcgatg
     9361 ccggcgccga gcttcatgtc cgcggcgatc cccgacagcc cctccaccac gcgctcgaaa
     9421 cgactcttgt gcacatacag gcgcgaaccc gcgcagcaca cctgcccctg gttgaagaag
     9481 atcgcctgcg ccgcgcccgc agccgctacc gccgggtcgg cgtcgtcgag cacgatcacc
     9541 gggctcttgc cgcccagctc cagcgacacc cgcgtcatgt tgtcgagcgc ggccttgccg
     9601 accagcttgc cgatctcggt cgagccggtg aaggccacct tgtcgacgcc cttgtgcgcg
     9661 gccagcgccg cacccgcgga gtggccgtgg ccggtgacga cgttgaacac ccccgcgggg
     9721 tagcccgcct gctgcaccag ctcggcaaag cgcagcgccg acagcggcgt ctcctcggcg
     9781 ggcttgagca ccatcgtgca cccggaggcg agtgccggcc ccgccttcca cgccaccatc
     9841 agcagcggga agttccacgg gatgatcgcc cctaccacgc ccaccggctc gcgccgggtg
     9901 tagccgaaga actcgcggtc gcgcaccagg gggaccgaca cctccatcgt cgagccttcg
     9961 atcttggtcg cccagccggc catgtagcgc aggaagtcca ccaccagcgc cacgtcgacc
    10021 gcacgcgcca tggtcaccgg cttgccgttg tcgagcgcct cgatctcggc cagttcctgc
    10081 gcatgcgcct ccaccagctc ggccaggcgc agcagcaggc gctcgcggtc caccggtcgc
    10141 atgcgcggcc agtcgccctg ctcgaaggcg cgccgcgccg ccgccaccgc gcggtcgatg
    10201 tcggtcgcct cgcccgccgg cacgcgatcg aacacctgcc cgctggcggg atccaccacc
    10261 tcgagcatgc caccgccgag ggcctccacc cattcgccgc cgatcgccat gcgcttgggc
    10321 tgcgccagga accggcgcgt gtcgtcgctc agcagttcac ccttgtatcg gtccattcct
    10381 cgtctccgtg tttgatgtgt gttgcccggg acggccgcag gccgccccct caatagttga
    10441 aaatcagcac cgcctgcaga tagacattgc ggcggtcgtt gccctgcagc gccttcacgt
    10501 ccgcaccgcg cggctcgtag aggccgatca ggggcacgaa ggtgaagtgc ttgtcgatct
    10561 gccagaacgc gaacaggtcg aactcgcgcc cggccaggtc cgcggcctcg ccctcgggcg
    10621 cgaatttcca gtactgcacg ccgatggaga gatcctcgcg gggtgccagg ctcagctcca
    10681 cgcggtccac ccgattgccg ctgttggccg ggccggcgta gttggacgcg acctcgccct
    10741 ggaaccaggt ccccaggccg cggctgaggc cgaagaacag cgggtcgaag gcctcgttgc
    10801 gggcggtggc ggacttgtcg gcggagaagc gtgcgtggcg gtagtcgagc gtggggcgcc
    10861 agggcaggtc ggcgaaggtc cacccgccct cgacgtgcca ggcgtcggcg tcgttcttca
    10921 ccgcggcgct gccgccgcgc tggtcgacgt actcgaaggc gaggaacagg ttctccacgc
    10981 cgaggctgcc ctggccgcgt acgctggcga tgcgcatgcc gtcgcgacgg tcgaagatgc
    11041 ccagcccggc gccctggtcg acgtcgagca ccttcatcca gctcagcccg agcgtgccat
    11101 gcgtctccga cacgtactcc aggttcgcgc cgccgagctc ggtgtcctgc tggtaggggt
    11161 tgcccgactg cagccagaag gcgtcgccgc gccagggccc ctcgggatcg atgcgcacga
    11221 tcgcggtgtt gcggaagctc ttcttggcgg cgaggtagta ggccccgccc cgatccacgt
    11281 ccacaccgag cgcatcgagc cccttgccga ggttgatccg gtcgctcgcg atcaggaagc
    11341 cgtcgcccag cacgaactgc tggcggccga acgagacgtc gaacgcgcca cctgcgctgc
    11401 gccagccgac gtacgcatcc tccaggtcga cgtcgcgctc gcggccgtcg gtatagccgc
    11461 cggcatcgcc gtcgccccag gtgccttgtg cgatcgcgcc caggccgccg tacagcttgc
    11521 cgccgccggc acgggtggtg cccgccagca cgccatggat gaagccctcg cgccactgcc
    11581 tgccgccctc gccgagatag tcctcgcgcg tggagaaggc gccggccatg ccgtcgatat
    11641 tcgcgctcag cgtggtgccg tcgcgttcgt gcaaggtctc tgcccgggcc gtgccgagcg
    11701 ccgccagtcc ggccagcagg acggccgtgg tgatgcgcat gcgcatgatg ttcgtctcct
    11761 cgatgattgt tttggcgcgc gggacaccgc ggtgcgccgc ttggcgcgcc gtggtgtccc
    11821 gctgctgcgg gcttgccccg tccggaagcg ggcgctcagc gcacgaagac ctgcccggta
    11881 ccgagcgaca tgtccccgcc cggcagcttc acctgcccga ccttggccag cgtgtcggcg
    11941 tcgtggatgg agatgtcgtt gaaggtgccg gtgaggtaga gccggctgcc ggcctggttg
    12001 ctcagcacgg tgtagtaggt gtggtcgagc tcggcggcct gcaccagctt ctgctgcgcg
    12061 atgtcgtact tggtcaggcg gtgcagcagg ccgtagacga tgttgcggtc cttcggcgaa
    12121 cgcagcccgg tgaagtagat ctcggtcagc ggtccgaact cgcgtgcctc ggtctcgccg
    12181 ctcttcaggt tcacgttcag atagccgtac tggtactcgg cggtcgccat gtcctgcttg
    12241 tcgtcctggt acttcgccgt ggtgtagagg atggtgaagt cccgcgtcgg cgtctgcacc
    12301 ggccagatgt acagcacatc gagcggcgcg tgcgtggaac gctgccagtc gcggatcggc
    12361 agggcgacgg tcatcgcacc ggtgttcaca tccaccttgt agaggttcgg tccggccagg
    12421 tagaccgagc cgtcctcacc cgcctgcatg atgttggcct ggcgcggcgc cggaaacaca
    12481 cgcacaggct tggcctccag accggcacgg gtatcgtaga ccgcgaagcg cggcgccccc
    12541 acgcgatagt ggtcgcgatg gatggtggtc gggttctgca ccgcatagac ctccttgccg
    12601 tccgcgctga cggtgaagga tgcgatcgac ttggcacgct cgttgtcgcc ctgcgacatc
    12661 tcggcgcgga aggtgagcct gcaggtgtcg agatcgatgc cgtagatcgt gccgaaacgg
    12721 ttgttgagga tgtaggcggt gcggcggtcg ggcgcgatct gcgtggtgcc gggtccgaag
    12781 gcgtcgggca gcgggcaggt cttgtagacg ctctcgctgg ccagatcgac cacgttcagg
    12841 ttgttcgggt agttggccac catcaggtat tcgctgcccg gctcgagcgg ccgcgcctcg
    12901 tttgccgctg cggcgggcag gactccggcg gccaggcagg cgagcgcggc cgcgctgcgg
    12961 cgcagtgcac cgccgatcat cgtcttcttc atgtccgccc ccttacagct tgtcgcccgg
    13021 gaagaccgag ccgagattgc gccagtcctc gcgcgagttg gtcgcgtcct tgttccagtc
    13081 cgggtaggtg ttcatcacgt ccggcacctg ggccggccac cagcacggat cggcgcagcc
    13141 gtagagatcg gattccatcg gctggcacag cgaggacaca ccgccgaagg cgtccacttc
    13201 ccagcccggg tccaccgtcg ccgcgcagcc ggcgatggcg ctcatcgcca ccacctcctc
    13261 gacccggtcc tccgcgaccg ccttgtccag cagccgcgcc ttgttgttga tcgccttgag
    13321 atgtttcatg acgccgtcct cgtggaaata tgctgttcga tgaaggccgg gttcccggcc
    13381 atgatgcggc tgtagacctc gatgccgaag tccacccagt cgcgcatcag atcgcagtag
    13441 tggtaggtgg gatgtgcggg atcgccgtag cgcgcgtagc tctcgtggta gcagccgccc
    13501 gagcacaggt tgcggatgcg gcagctcgcg cagccggtat ccgtgcgatc caggcgctgg
    13561 gacaggaaat cgccgagctg cgcgcgctgg ataccgttct tcacgtcgcc gaaggtcggc
    13621 agcgtggagc cggtgaagcg atggcacagg ttcagttcac ccttgtggtc gaccgcgagc
    13681 atcttcaacc cggcgccaca gggcagcgcc ttcttgtggc cttcgtgcag gtcggtgatc
    13741 agctggtgca tgttggagaa accgatgttg cgtccctcca gcgccgcctc cagatagcgc
    13801 gcaccgagcc gcttcagccc ggcgaagacc tcgaccagct ccgcgcccgt gaggttgaag
    13861 gtgctgatgt cgcccgaggt caccggcgcg aagcccactt cggcaaagcc cagctcgttg
    13921 aacaggtggt cccagatgcg ctcgacctcg gtggtgccgt gggtgagcgt gacccgtgcc
    13981 cccaccggcc gcgcgtggta gcgcgccagc aggcgctcgg ccttgcgccg cacggtctcg
    14041 taggtgccct ggccgccgac ggtgagccgg ttgcggtcgt ggatcgcctt cggaccgtcg
    14101 atgctgatcg acagcccgaa gcggtgggca tcgagccagt cgatggtgtc gtcggcgagc
    14161 agggtcgcgt tcgtcgtcat gacgaaatcg acctgcttgc cgagctcggc gaagcgcgcc
    14221 tcgcagtacg cgaccacgtc cttgatcagc ggcaggttgc tgagcggctc gccgccgaag
    14281 aagacgacgt tgtagcgcgg ctcgtccggc gactcgcgca acatcatctc gatcgcgtcg
    14341 cgcgcggtcc cgaaggccat cttgcggccc gccgagggct tgtcgaggtc ttccttgtag
    14401 cagtaggtac agctcaggtt gcagccggtg ttgacgttga gcaccaccgt ggtgagcggg
    14461 aagcgctcga aggtgtgcgc gagcgtgccg gcgctggcgg ggcggccgtc gtcgatgagc
    14521 tcgagcgcga ccagctcgcg gatcgcctcg gccacctcgt cggcgtcgaa ctcggtacgc
    14581 aggccatcga tcagggcctc gacggtgagg ctctgacggc ggatgcgatc gatcagcgcc
    14641 gcgctcagcg ggtcgagcgc gaacagcgaa ctgctcggga tgtggaacag catgcgcgtc
    14701 tcatcgatgc gcagctcgtg caggttgtcc tgcaccagat tcaagacggc acccatggga
    14761 gcctccttcg gggtcgggat tgcggagggc aggccgcgcc tgccggaaca ggacgcgcca
    14821 ccgatcaggg cagcggcggg ttgttccagc gcggcggcgc cacgatcacc tggccttcgc
    14881 cgcgcaggcg ttcgccaccc tgcgccacct cggccaccac cttcaggttg cccacgttgc
    14941 tcgccgacat ccgccgcacc gggttgggac cggcatcacc cggcacgaag accccgctcg
    15001 cggcatccat caggccggcg aactcgacgt cctgttcgcg caccgccgcc tcgtcgaagg
    15061 gctcgaccga ccacttcgcc ggcaccacgc cgatgcggaa gtcgtcctcg gtgcctgcct
    15121 tgccgtccgc gccggctgcc caggcctcgg cgtcgaagcg cgcctcgacc ttggccgtgg
    15181 gcgtgccgtt gccgccgatg cgtgcgaccg cgaacgcggg cgacaccttg accgcggcga
    15241 tgctgtcgta cagcgcgagg ctgccgcccg aagccttgcc gaccgccacc gcgtgcaccc
    15301 cgcgcgccgc ggcgtcgctc tgcacgcgga ccacgacctc ggtcgccgat cgcttgaccg
    15361 taccgaccag cttcacgccc tgcggcagcg tcacctcgcc cgccagcccg gagccgacga
    15421 tgcgcagttc ggcgccttca cccgccttga ggtaggccgg atgcaccgcc agcacgcggc
    15481 tgttgcccgc ctgttgcagg ctggcgacga cgtcggcgcc gacctcgtcc tggtcgcgca
    15541 ggaacatgcg gccgcgcatc atgccgtcct cgaccgcgaa cacctggcgc atcggcgtgc
    15601 cgtcgacgac caggtcgccg cgccactcgt aaccggtgta gatcagcgcc cgccccctgc
    15661 cctgcatcgg cctgccatcg tcccaacgcc cgtcgaaggc gagcgtgtag cggtcccggc
    15721 cctcacccgc cgtcaccttc atcacgcccg agaatccgcc gcggccggtc agatggccgc
    15781 tcaggctcca ctcgcccttg accgtctgcg gcgcgcgcgc ctgccactgc ttccaggcct
    15841 cggactgcaa cggcagcttg ctcgcaagct ccggcaccat ttccttcaac gcgatgccga
    15901 accagtcgcg gtcgcggccg agggcctggt actcggtcgt cgggaactgg ccgagatgga
    15961 aatgcaccag atgctcccac tccgccgccg ggcgacgctg cagcatcacg cgcgcaccgg
    16021 aatggcagcg cgcgcacatc tgggtgaact tctccgactc gaaggcctcg acggtattca
    16081 ggcgacgctc catcgcgtag cgcacgccgg tggtctcgga cggcgccagg ccctggcggt
    16141 cggccaggtg cttgaccacc gtgcggcgtt cctcgtcggt gatcttcagg ccgtggacga
    16201 tctgcatgcg tgccaccgac atcagccagc cttccggggt cttgcgctgg tggctgatgc
    16261 ggctgatgcc gtcctcggtt tcggtgtgac agggcaggca cttggcgcgg atcagcgctt
    16321 cggccgaggc atgcgccggg ggggcggcca gcacgacgcc gagcgcagcc atactgccca
    16381 tggcagtggc aagggttctg attttcaaga ccggatctcc tcttcgtgtc cttggatgag
    16441 gcgtccaccc ggtgggccgg catcgccgtc ccgtgcgcgg ggaacaccgc agcgctgcac
    16501 agaccgaggg caagcacgga caccgctcgc gacgggcgat ccgatcctgt ctcctcctca
    16561 cgccaccgca aggtcacgcg agtccgcttg ctgcccttct gcgagggcct gcacaactgc
    16621 tgcctctgcc tccgcacgcc cggtcggcgc cgggtcgatg tggaaggcga gcagatcgta
    16681 ggcgcggacc ttccgtgcgc gttatccgaa aacggcaagc cacggcggcg gccggtcccg
    16741 gaccccgggt tgcaaaacga cccgccgccg tgccattctg aaaaacggcc gcacagcact
    16801 ggggaagcgt tcatccgcac cctgagcggc agggacaaac ataacgacaa gcacgatcgg
    16861 ccaccgatcg agagaggaag agacatgttg gccaacgcca cccggcaact gcccaggggg
    16921 tgcgggcaat gagcccggct gcccagcctt cgggaaacgc cccgtactcc tacaccctgc
    16981 gccacacgct cgatgccgac gagcacgccg cctgcctgac caactggcat caacgctacg
    17041 accagctcac cgcgggcgcc ttcgacggcg tgttcgagga gttctgcttc ggcagggtgc
    17101 agctgttccg cgagggcctg aaccagtcgg tgcatcaggc tggcggggcg tggcccggct
    17161 cgcgcacctt cgccgtgccg gtcgcgatcg agggcacggg ctggttcggc ggcgagatgt
    17221 atgacgcgca ctccatgctg acactgggag gcgacgacga gctcgacttc cgcaccccgc
    17281 gccggctcga gatcctcgcc tgcaccgccg actcggccgc gctccacgac tacgcgcatc
    17341 aggtggacca ccgcaacctc gaggccgagc tcgccgggcg caagctggca ccgaccaccc
    17401 cggcgggaat cgcggcgctc gggcaactgc tggcgaccat gacggccagc ctgcgggcaa
    17461 cccccgagct gcttctgcac ccgcagatgc gcaaggcgat ggaacaggcg ctgttcgcga
    17521 ccctgctcga cacgctctcc tgtggcggcg gacgggccgg tgcaccgtcc tgtcgcgccc
    17581 gccagcaggt ggtggcgcgc gcgcgcgcct acatggaggc ccacatcgac gagccgatca
    17641 cggtcgccga cctgtgcatc gagctcggca tctcgcgccg caccctgcag tacagcttcc
    17701 aggacgtgct cgatctcaac ccggtcaagt tcctgcgcgc gatccgcctg aatgcggtgc
    17761 gccgcgcgct caaggccgcg gaccccaatg gtcgcggcac ggtcgccgac atcgccgcac
    17821 gctggggctt ctggcacctg tcgcacttct ccgccgagta caagacgatg ttcggcgagc
    17881 tgccgtcgga cacgctcagg cgcgcgggct gagcaggcgc ggcgggcgaa aaataaaatc
    17941 ttttgctgga accattagcc caaatcggca acacgctccg ccgtccggga gacgaaatgg
    18001 atgcaggcgg cgctcaatcc agccagttct gtgcccgcat ccaggccagc aaggcggcgg
    18061 cacgcgcacg ctcgagcccg aaacgcgcgc acagcagcgc ctcgccggca gctttttcca
    18121 gcgcatcgcc tccctctcga cgcaaggcgg caagcatggg cgccaccgcc agaccatcga
    18181 ggtgccaaac gccgagcggc tggtcgggcg tcaccacgac gtcttgctcg acgatccgcc
    18241 ccgcgcacac caccggcccg cgcgcaacac gcacggtctc cggcctcacc ttggcgtgca
    18301 gcggcagggc atccggccag ccgcggcgcg cggcccagaa cggcgcctgc ggccagcgcg
    18361 cttcctgagc gtagaaatcg cgcccgattc gggcgaagcg gtggaacagg tgctcgatac
    18421 gccgcgtatg gaagtgctgc gcgagcgcgg tgcggccccg atcgtgacgg agggtagcca
    18481 ccaccgccgg tgcctgcagc gcggacgaca gcgcctggaa gatgccgttg cccgagagcg
    18541 gatcgaccgc catcgccgca tcgccgacgc ggatccagtc gtcgccggcc accgattcgt
    18601 tgagcaccgc cgtgctggtg cgcgcgtgcg gttcgccgac cggctgcgca tcgcggacga
    18661 agggcgccgc cgcctcgacc gcgtgaaacc gtgcgctgca gtagtcgccc agggcccttt
    18721 tcggcggcag gtcggcgctg gcaacgtcca ccgtcagctg cagatagcgc cgtccatcgg
    18781 ccaacgcggc catccacatc cagccatcct cgacgctgag aacggtcgag cccgcctcgc
    18841 ccgccggtcc ctgccagtac tgcagcagcg acaccgtctc cactccgcgc acccgcggcg
    18901 cccccccgcc gggcgcggcg cggccgcgcg cctcgacgag gaagcccgcc gccatgcggc
    18961 gcggcccggc atcggtgtcg atctccagct catggccccc tggcgcgggg tgcagcgccg
    19021 tcacccggcc gcgcaccacg ggcacgccga ggcgctccaa gtcctcgagc agcccctggt
    19081 cgaagcgctg gcggtcgacc aggctctcca cgttcgcctc ggtgtaggtg ccattccaca
    19141 ccgcccgccg cggcgaaggc ggcgcgaacg ccgtcagcgc ccgctgcagg ccgaggccgc
    19201 gcagcgcgtc gatgacgcgc gccgagacgc cctcgaccgc ggcgaaacgg cgcggctcgc
    19261 ccaccagcac caccggctcg cccatccgcg ccagtccgat cgccaccgcc gcgcccgcgg
    19321 ggccggcgcc gaggatgacg atgccgtcag ccgccatgct gtcttctcct cgggtcgtcc
    19381 acaccgtggt ggcgcgcctg ctcgaccagc caggcacgca gtgcgtccgc gtcgccgccc
    19441 cgcgcgaagt gccgcgcggc cagtccggtg aaccaggcac aggccatgct cgcacccgcg
    19501 ccggcgaggc taccgtcgag cggccgcaca tgggcaccga aatcgatctc ggcggagccg
    19561 atcgcggcga gctcgccggg tgcgcagcgc gcatctccgg tcacccgcag cacgcccgga
    19621 aaacccgccg gatacacggt ctggccgcgc gcgggcgccg ccgcgcacag cacggtgccg
    19681 gccgccagtg cgcgcgcgca ggcctcggcc agcaccggcc gcggttcgcg caggcccaga
    19741 ctcagattga cgagctgggc gccgttcgcc accgcccagt cgatcgccgc cgccacctgc
    19801 gccgcggtgg tcgtgaggcg ctcgcggaac acctggacga ccagcagctc ggcctgcggc
    19861 gcgcaatgca gcaggatccc ggccacgcga ccgccgtggc cgagcgcgtc ggcggtggcc
    19921 ggcgcctggc gcacggcacg gtcttcgagc accagggcga ccgcgtcccg cacctgcagc
    19981 gcctgtgctg gcgtgcaccc gctatcgacc acggcgatcc gcaccgtcat gaccgcaccg
    20041 tcaccgccgc cggcggcagc acctcgagcc gcccgcgctc gaggtgcacg cgcagatcgc
    20101 aaccggcgag cgtcgccgcg cggtggctga tcaggatccg cgtgcgtccg gcgaagagcg
    20161 catcgacctc ggcgatgatc gccgcctcgg tcgcctcgtc gaccgccgag gtcgcctcgt
    20221 cgagcaccag gacgcacggc tcctgcagca gcgcacgcgc gatcgcgatg cgctggcgct
    20281 ggccgccgga gagctgctga ccgcgctcgc cgagcgctgt ctccagcccc agtggcaggc
    20341 ttgcgatcaa ggcgtcgagg tgcgcccgcc gcgccgcggc acgcaccgcc ccctcgtccg
    20401 cctgcggcgc ggcgtagcgg atgttctcgg cgagcgtgcc gcggaacagc acgatgtcct
    20461 ggctcaccac cgccaccgcg cggcgcagcg cgccgagcgg cagcgcgcgc aggtcggcgc
    20521 cgtcgagccg gatggtcccg gcctgggggt cgtaatgacg gtgcagcagg tcgatcagcg
    20581 tggacttgcc gacgcccgaa gcgccgctca gtgcgacctt gctgcccgcg ggaaccgtca
    20641 gccgggcgcc gtcgagcacc gcctcggcgc gtccctggtg gtggaagacg accgcatcga
    20701 actcgatccg gccctgccac gccggcacgg cccgctgtgc cgcgccgggg tgcgcgccat
    20761 ccacgacgtc ggccggcgcc tcgcgcagct cgctcacccg ccgcagactc acgctcatgc
    20821 gctggatcgc gacatacagg ccgagcaggc tgttgaccgg cccggtggcc atgccgaggt
    20881 aggtggagaa cgcgatcagc gcgccgagct gccagctgcc ctcgatcacc caccagccgc
    20941 cgatcatgaa cgcggcggcg cgcgtcgccg aggtcagcgt ggacgggatc gcatgggtga
    21001 ggaactcggt gcgctgcagg cgcagcagat cgtcgaggta gcgcgcgccg agatcgtcga
    21061 gccggctgcg ctcgtcggcc tcgcggcgcg aagcctggat gaacttcatc gccggcaggg
    21121 tctcgaccag aaagctcgag acatccgccg cgcgctcgcg cacggtgcgc gtgcgcgcct
    21181 cgaccttgcg ccgcatccgc cgcaaccaca gcgtctcgag cggcaccagc accagcacca
    21241 gcaaggacag cttccacgac aggctcagca tcagcgccac cgcgccgagc aggccgatca
    21301 cgctgctcac cgcggcgaac aggctatcca ccgcgaaacg ctggatctcg gcgacgtcgc
    21361 cgtcgaggcg cgacagcagg tcgcccatgc gctgacggcc gtagaaggcg ggcgacagcg
    21421 tctgcaggtg gcgatagagg tcgctgcgca gcgcgaacag gatggagccg gacagacggg
    21481 tgtgcaattg ccggttcacc ccggcgagca cggtgccgac gatgccgacc gcgatcatcg
    21541 cgccggcgac catcagcagg gtgcggtagt cgcgcgcgag caggccctcg tcgatcagca
    21601 gcttggtcag ccagggctgc agcagcacca gcagggtggc cgcgcaggag agcagcagca
    21661 ggccggcaat gcggcggcgc tgcgggcgca cgaagccgta cagccagcgc aggccgcggc
    21721 gcaactcgtc gggccgctct gcatggatca ggccggcgag catgacccgg ctcgcgacga
    21781 cgacgcgtcc atcgggcccg ctgcgcattg gcggtcgcgc gcgacgaaga agcggccagg
    21841 cacgcgcatc gcccgcctca gctcgcgacc agcatgccgt gctcgcgccg cgccacgccg
    21901 gcgcgctcga gctccttcac cagccaggcg gcgagcgcat cggcgtcgag gccgaggaag
    21961 cgcgcgttgg cggtgcggta cagcggggtc tcgtcgagat gccgcggcag ctcgacgagc
    22021 gccatgctgc gcacctcgag cagcttgaag gtgatgcagg cgcggatcgc gttgcgtgcc
    22081 atgcgcgcgc cgtcgtcctc gaaggcgcgg aggcgcgcga aggcgcgctc gagcgcgtcg
    22141 tcgaattcgg cgaagggggc cccatggccg ggaatcaccg catccaccgc gagccggccg
    22201 atcgcctcca gggtgcggcg cgcctcgccg agcgcgtcgc cctggcccat cacctcggcg
    22261 aacaggatgc cgaagccgtc gcgccagagc gcatcgcccg agatgagaat gcggcgatcg
    22321 gggttgtagt aggcgagcgc gtccatgtcg tggccggggg cggccaccgc ctgccattcg
    22381 agctcgccgg cgacgaagcg cgccccggcc gcgagcgtcg cgtcatggcg gaagcgctcg
    22441 ccggcctgcg cgccgacgct cagcagcagc gcctcctcgt cccactcctc gaccgcgcgc
    22501 gccatgccct cgggcaccgt gatgctgcag ccgaaggcgc gctgcacgct ggcgttgccg
    22561 ccgatgtggt cggaatgcga gtgcgtgttg atgaggcggc cgagactgcg gccatcgagc
    22621 gcggcgcgca ccagcttcac cgtctggtcg gcatggctca cgtagccgga gtcgatcagg
    22681 gtcgcctcgt cgccgtcgaa cagcagcacg ttgttggccg acagccagcc gcgctcgaag
    22741 acctgcaggc tggcaggcag atgcggacgc gcgctcaatc gctgccttcc ccgacgcggg
    22801 ccgcgacgtt gggcggcaag ggcacgccgg ccgcctgcgc cggggacgcc gccgcatcgg
    22861 gctgccccgc gacctgcgct ccgggcgccg gcgtgggcac gccgttgatc tcgtcggcag
    22921 tgcgaccaca gcccaggcac acgcccgagt cccaatcgat catgcacacg ccgacacaga
    22981 gcgcgtcgcc gctcatgtgc gcaagtccac ggcgggggaa gccgcggcca ggcgcgtgcg
    23041 gcgctcggcc acggcggcga ggatggcgcg gcggtcgtcg tcggtgctgc ggctccagcg
    23101 cgtgatctct tcgatcgtgc gctggcagcc ctcgcaccag cccgtgtcgg gactcatgcg
    23161 gcagaggttg atgcaggggg aaggaacgct catggcagtc atctctgtag ggcggcgggc
    23221 gggccatgcg tgtggtccgc ctcgtgatcc ggaagccgga ttatgaagca gtcggcaagc
    23281 catggctcgc gaggaccgcg ttcgaccgcc tccccgcccg cgtcgcacgc accctccgtc
    23341 gcttcatccc gcgctcaggc gcaggccgtc ttggcgcgct tggccagcac cgcacgcgcc
    23401 acacgcgagg caggctcgcg gccaagctgg gcgctgatcc aggcggcggt gtcgacgagc
    23461 gcgtcgagat cgatgccggt gtcgatgccg agcccgttca gcaaccagac cacgtcttcg
    23521 gtggcgacgt tccccgaggc gcccgccgca tagggacaac cgcccaggcc gccgaccgag
    23581 gcgtcgaaca ccgccacgcc catctccagc gacgcgaaga cgttggccac cgccatgccg
    23641 taggtgtcgt ggtagtggcc ggcgagcttg tccaccggca cgcgcttcgc caccgcttcg
    23701 agcatgcgcc ggatgccggc cggggtgccg acgccgatgg tgtcgccgag cgagacctcg
    23761 tagcagccca tctccagcag cgtcgccgcc acgtcggcca ccgcctgcgg cgcgaccgcc
    23821 ccctcgtagg ggcaacccac cacgcaggag acgtagccgc gcaccctcac gccggccgcg
    23881 tgcgccgcct cggtgaccgg gcggaagcgt tcgagcgact tggcgatcga gcagttgatg
    23941 ttcttctggc tgaaggactc ggaggcggcg ccgaacaccg caacctcccg tgccccggcc
    24001 gccagcgcgg cctcgaagcc cttgaggttg ggggtgagca ccggataggc gacgccgggc
    24061 gccgcagcgg cgagcacgcg cggcagcagc tcggcgttgt cgcccatctg cggcacccac
    24121 ttgggcgaca cgaaggacgt ggtctcgatc gccttcagcc cggcgcgctc gaggcggcgc
    24181 acgagctcga gcttggtgtc ggtggagacg agctgcttct cgttctgcag gccgtcgcgc
    24241 ggccccatct cgacgatgcg gacggcggcg ggaaggatgg aattcgccat ggagggggtc
    24301 tcctcaagaa gcgttcaatt gggctgcagg agcggcgatc agccgtcttg gccgatgccg
    24361 ccacgccgac aagaacacgc gagcacgaca gacccgcgat caggcggtct tcgcctcgtc
    24421 gagcccgagc tcttccttca tcctctggcg gatcaggaac ttctggatct tgccggtcac
    24481 cgtcatcggg aagctgtcga cgaagcggat gtagcgcggg atcttgtagt gggcgatctg
    24541 gcccttgcaa tactcgcgca cctcgtcctc ggtgaggcgc tcgccctcgc gcgggatgat
    24601 ccaggcgcac agctcctcgc cgaacttctg gtcggggatg ccgaccacct gcacgtcgag
    24661 caccttgggg tgggcgtaga ggaactcctc gatctcgcgc ggatagatgt tctcgccgcc
    24721 gcggatcacc atgtccttga tgcggccgac gatgttgcag tagccctcgt cgtcgatcac
    24781 cgcgaggtcg ccggtgtgca tccatccgcc ggcgtcgatc gcctccttcg tcctggcctc
    24841 gtcgtcccag taaccgagca tcaccgaata gcctcgggtg cacaactcgc cggactggcc
    24901 gcgcggcacg atgcggccct cgttgtcgac gatcttcacc tcgagatggg gctggatgcg
    24961 gcccacggtg gatacgcggc ggtcgatggg gtcgtcggtg ccgctctgga agctcaccgg
    25021 cgaggtctcc gtcatgccgt aggcgatggt cacctcggac atgttcatct tgccgatcac
    25081 ccgcttcatg acctcgatcg gacacgggct gccggccatg atgccggtgc gcaggctgga
    25141 aaggtcgaac tccgcgaagc gcggatgatc gagcgcggcg atgaacatgg tgggcacgcc
    25201 gtagaggccg gtgcattttt cctgggccac ggtctccagc accgccaggg gctcgaaggc
    25261 ctcgcccgga tagaccatgg tggcgccgtg ggtcaggcag ccgaggttac ccatcaccat
    25321 gccgaagcag tgatagagcg ggaccgggat gcacagccgg tcgccgggca tcagcttgat
    25381 cgcctcgccg acgaagaagc cgttgttgag gatgttgtgg tgcgacaggg tcgcaccctt
    25441 ggggtggccg gtggtgccgg aggtgaactg gatgttgatc gggtcgtcga actgcagctt
    25501 ctcggacagc acggcgagcg cggtcagttc ctcgcggctg ggcgcgcgca gcaagtcgtc
    25561 gaagctcatc atgccgggcg acttctctgc gcccatgcgg atcaccatct ccaggctggg
    25621 caggcggtgg ctgcgcagca ggccgggctc gcaatggccg agctcgggcg caaggtcggc
    25681 gatcatctcg aggtagttgc tggtcttgaa ggcgggcgac aggatcagcg cgcggcaacc
    25741 gaccttctcg agcgcgtatt cgagctcgga gcggcggtag gcggggttga tgttcaccag
    25801 caccaggccg gccttggcgc tggcgaactg ggtcagcgcc cactccatgt tgttctgcga
    25861 ccagatgccg atgcgctcgc ccggttccag gccgaggcgc atcaggccgc aggcgagcgc
    25921 gtcgaccttg ctcttgagct gcgcgtaggt caggcgcacg tcctggtgac gcaccaccag
    25981 cgcctcgcgg ccggcgtggc gggcgcaggc ctcgtcgaag aagcggccga tggtctggcc
    26041 gatcaggggt ttggcggaag cgccgtgcac atagctcgca gtgctgctca tcgtgtcgtc
    26101 ctctctcctg ttcttgtcgt cgcgcggcat gtcgcgctcg caatcctgtc catcccgtgc
    26161 ccgcgccacc acggaaccgc ttcattcgcg acttgcgtcg ctcctacacg gcgccccaga
    26221 cgcgcgcacg gtttccgtca tcaccgaccc gccccatcgc aagcgggcgg ggagccccac
    26281 acgcgcgcgg ttttccggca tcgccgacgc accccatcgc aggtgggcag gaagcgccag
    26341 atgcccggcg cttcccgtac gagcgacgca agtcgcgatc gggcggctgc gcggcacagg
    26401 cgtggcgccg gtgcgccctg cggcatgctt ggacgcctcc tgcggaaaac cgccgcgcct
    26461 cgggggccat gtgggagcga cgccagtcgc gattgccgcc gggcacccgc gaccgcgccg
    26521 aacaggcgca ggcgccccgc cacggcgttg gcctcaaacc gccgcggctt cgaactccac
    26581 cagctcggca ccgtcgccga cctggtcgcc gacgccgaag cggaaggcct tgaccgtgcc
    26641 ggccgccggc gcggtgatgg tgtgctccat cttcatcgcc tccaggatca gcagcggcgc
    26701 gcccttctcc accttcgccc cctctgcggc gaccagcgcg atcaccttgc ccggcatcgg
    26761 cgccatcagc ccgccctcgg cgccaccgcc ctcgccaccg tggtgcagcg ggtcgaccgc
    26821 ggccagttgc caggcgcggc ccctggcgaa gacgtggcga cggccggcgg cggcgatcac
    26881 ggtggcgtcc atgcgggtgc cgtcgagctc gacccgcagc aggccgcgcg ggttgagctc
    26941 gccgcgcgcg accacgctgc tgctgcccac cgtcagccgc caggcgccgg gcaggtaggc
    27001 cacctcgacc gcgcgctcgc tctcgccgtg gcggaacagc aggctgcggc aggcggtggc
    27061 gttcatgcgc cagccgtcgc gggcatgcca gggcgaggcc ggatcggcgc cgcgcgccgc
    27121 gcggcggtcg gcgaaggcgg cgtcgcgcag cagctcggca agcgcggcga tctgcaggac
    27181 ctcggccggc acggcctcgt cggctggaaa caggtaggcc ttctcgcgct cgatgagccc
    27241 ggtgtcgagg tcggcgccgg cgaaggccgg acaggccgtg aggcgcgaca ggaactcgat
    27301 gttgttggcc acgcccacca cccggtagtc ggcgagggcc tgcagcatgc gggcgagcgc
    27361 gcggtcgcgg ttgatgtccc acacgatcag cttggcgatc atcgggtcgt agtgcgggct
    27421 gatctcgtcg ccctcctcga cgccggtgtc gacgcgcacg tgcaggctct cggccggcgg
    27481 cgccagatgc accagcttgc cggtcgaggg caggaagccc ttggccgggt cttcggcgta
    27541 gatgcgcgct tccagcgcat ggccgcggat ctgcagttgc tcctgcgcca gcggcagctt
    27601 ctcgcccgag gcgacgcgca gctgccactc gacgaggtcc agcccggtga tcatctcggt
    27661 gaccgggtgc tcgacctgca ggcgggtgtt catctccatg aagtagaagg agccgtcctg
    27721 gttggcgatg aactccaccg tgcccgcgcc gacatagccc accgccttgg ccgcatcgac
    27781 cgcggccttg cccatcgcgg cgcggcgctc gagcgtcatg ccgggcgcgg gggcttcttc
    27841 gagcaccttc tggtggcggc gctgcaccga gcagtcgcgc tcgaacaggt acaccacgtt
    27901 gccgtgggtg tcgccgaaga cctggatctc gatgtggcgc ggcttggtga tgtacttctc
    27961 gaccagcacg tggtcgtcgc cgaagctgga gatcgcctcg cgcttgcagg aggcgagcag
    28021 atcgatgaag tcctccgacc tgtcgaccag gcgcatgccc ttgccgccgc cgccggccgc
    28081 agccttgatc agcaccgggt agccgatggc gtcggcctgc tggtgcagcc aggccgggtc
    28141 ctggtcgtcg ccgtggtagc cgggcgtcag cggcacgccg gccgcctcca tcagcttctt
    28201 ggcctcggac ttcgagccca tggcgcggat cgccgacacc ggcgggccga tgaagacgat
    28261 gccttcgcgc tcgcaggcgt ggcagaaatc ctcgttctcg gacaggaagc cgtagccggg
    28321 gtggatggcc tgggcaccgg tctgcttcgc ggcagcgatg atcttgtcga tcaccaggta
    28381 gctctcgcgc gccgccgccg ggccgatcag caccgcctcg tcggccaggc gcacgtggcg
    28441 ggcattggcg tcggcctcgg aatacaccgc gacggtcttg atgcccatgc ggcgggcggt
    28501 cttgatgacg cggcaggcga tttccccgcg gttggcaatc aggatcttgt cgaacatttc
    28561 gtctccagcg tgtcttgtgt cggttcggca cgtcggccgc tcaagcctgc gcgatccacg
    28621 ccgcggggcg cttgtcgaga aacgccgcca ggccttcctt ggcctcgggc gtggcgcgca
    28681 ggctggcgat gcggcgcgcg gtgtcttcca ccaacgcatc gctcagcggc cggttggcca
    28741 ccgcgcggat caggtccttg gccgcggcct gcgacttcgg accgccctgc agcagggcct
    28801 cgacgacctc cttcaccttg gcgtccagtt cctcgctcgc caccgcctcg tgcgccaggc
    28861 cgagttcggc cgcgcgcgcc gcgccgatgc gctcggcggt ctggaagtag cgggtggcct
    28921 gacgctcgcc gatggcgcgg atcacgtagg ggctgatcgc cgacgggatg atcccgaact
    28981 tgacctcgga ggtcgcgaac accgccttgt ccccggcgat gcagatgtcg caggcgctcg
    29041 ccaggcccat gccgccgccc agcgccgcgc cgtgcacgcg cgcgatcgtg ggcttgttca
    29101 tctcggccag ggtgcgcagc atgccggcaa gccgcatcgc gtcggcgaag ttctccgcct
    29161 cgctcgcctc gccggcagcc ttcatccaat tgaggtcggc gccggccgag aagctttttc
    29221 cccgcccggc cagcaccacc gcgcgcaccg cctcgtcggc atccagttcg atgcaggccg
    29281 tcgtgaggtc ggcgatcagt tgcgcgttga aggcgttgtg cacgtccggg cgattcatcc
    29341 agatcgtcgc cacgccggct gcgcgcacga tctccagggt ctcgtagctc atctcgcccc
    29401 cttacatccg gaacacaccg aacttcgtct gcgggatcgg cgcgttgagc gtggccgaca
    29461 gcgacaggcc gagcacgcgg cgcgactgcg cggggtcgat cacgccgtcg tcccacaggc
    29521 gggcggtggc gtagtaggga tgaccctgca cctcgtactg ctcgcggatc ggcgccttga
    29581 aggcttcttc gtcttccttg ctccaggtgc cgcccctggc ctcgatgccg tcgcggcgca
    29641 ccgtggagag cacgctcgcg gcctgctcgc cgcccatcac gctgacgcgc gcgttcggcc
    29701 acatccacag gaagcgcggc gagtaggcgc ggccgcacat gccgtagttg ccggcaccga
    29761 agctgccgcc catgatcatg gtgatcttcg gcacctgcac ggtggcgacc gcggtgacca
    29821 tcttggcgcc gtccttggcg atgccgccgt tctcgtactt gcgcccgacc atgaagccgg
    29881 tgatgttctg caggaacagc agcgggatgc cgcgctggcc gcacagctcg acgaagtgcg
    29941 cgcccttctg cgccgactcg ccgaacagga tgccgttgtt ggcgacgatg cccaccgggt
    30001 agccgtagag gcgggcgaag ccggtgacca gggtggtgcc gtagcgcggc ttgaactcgt
    30061 cgaaacgcga gccgtcgacg acgcgcgcga tgacctcgcg cacgtcgtag ggcttgcgcg
    30121 ggtcggcggg gatgatgccg tagatctcct cggggtcgta ggcgggttcc tcgctgtcgg
    30181 cgagcgccag ttcgacctcc ttgcgctggt tgaggttgga gacgatgcgg cgcgcaatgt
    30241 ggagcgcgtg gctgtcgttc tcggcgaggt agtcggccac gcccgagatg cgcgtgtgca
    30301 cgtcgccgcc gccgaggtcc tcggcgctca ccacctcgcc ggtggccgcc ttcaccagcg
    30361 gcggaccgcc gaggaagatc gtgccctggt tcttgacgat caccgcctcg tccgacatcg
    30421 ccggcacgta ggcgccgccg gcggtgcacg agcccatcac caccgcgatc tgcg
//
LOCUS       DAOQBS010000025        29552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_935_length_29552_cov_16.233278, whole genome shotgun sequence.
ACCESSION   DAOQBS010000025 DAOQBS010000000
VERSION     DAOQBS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 29552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29552
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_935_length_29552_cov_16.233278"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /locus_tag="PKN25_04860"
     CDS             complement(<1..1760)
                     /locus_tag="PKN25_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944786.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNV90263.1"
                     /translation="MTDLSVAHKLSPYKPKNKVRFVTAAALFDGHDASINIMRRILQS
                     TGSEVIHLGHNRSVGEIVNAALQEDVQGIAITSYQGGHVEFFKYMIDLLRANGGENIK
                     VFGGGGGVIVPAEIKELHEYGVTHIFSPEDGAKMGLQGMINSVVERCDVDVAKAAPQK
                     ADAVLKALAAGDRRALAQIITALENGGYGDEVKKALIEAAAKTKVPTLGITGTGGAGK
                     SSLTDELVRRFRLDQDDKLKLAIVSIDPSRKRTGGALLGDRIRMNAIEHANIYMRSLA
                     TRDTGSEVSAALPEVIAACKLAGFDLVIVETSGIGQGNAAIVPFVDLSLYVMTPEFGA
                     ASQLEKIDMLDFADFVAINKFDRKGALDALRDVAKQYQRNRELFNQRPEEMPVFGTMA
                     ARFNDDGVTALYQAVLPALVGKGLKVAKSKLPVVKVRASSEGRAIVPADRTRYLAEIA
                     DTVRGYHKHIEQQARVARERQSLKIAKGLFEQCGKDAGSFAELIDWKDGELTPAAKKL
                     LDMWPKTRELYAADEYVVKIRDKEIRTQLTSTSLSGSKIRKVALPNFEDAGETLKFLM
                     KENVPGSFPYTAGVFAFKREG"
     gene            2151..3230
                     /locus_tag="PKN25_04865"
     CDS             2151..3230
                     /locus_tag="PKN25_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNV90264.1"
                     /translation="MLAGLQRRGLDGAPLLARAGIDLAETDTRIPVERYALLYNLCVE
                     ALEDEAFGLLPEPMRPGSFEFLCRALLGAPTLGEALLRAIRFLRIVLPAFRIELRVDG
                     ARAELLLDDGGSLGPGLDAPARVFAYEWLLRLLHGVASWFVGRGLALDAVAFPYARPA
                     HADDYALVYTEHSSFDAPQLAARLQANLLALPLRRDEAALVGFLEGAPGKITTLYRRD
                     REMVFRVRDILRDALPQNLSLEEVAERLHVSPRTLHRRLEDEGSGFRNIKEATRRDIA
                     YARLAKTRQPIARIAAELGYADPSTFYRAFVAWSGMSPEQFRHRLAGNDSLPAASAGP
                     DRRPAPTPRVTAGRQRSREFGPTEA"
     gene            complement(3201..6179)
                     /locus_tag="PKN25_04870"
     CDS             complement(3201..6179)
                     /locus_tag="PKN25_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HNV90265.1"
                     /translation="MPAPPENLRVFVASPGDVAAERKLALEVINGMQRRWFARGKVTL
                     DTVEWDDDLAQVPMAASATPEDSVIDYLGRPRDCELTVVILWSRIGTRLPNSFRRADG
                     SAFESGTVWELEDARDRGRDVWVYRCTRPLEISGDDLKAQRVDEKLAQYRAVEAYFVR
                     AFQNPDGSLKGGVNTFDTPTSFGKALGEHLEAFITRRIDALRGASPRAPAGGAGAGAK
                     PWLVREPVRDYHVVGRAELVDRVWRKLRDGVDTSLLFLPGVGKTTVAQELLRERDKIL
                     GHFDGVLWANLGRQHVDSAELRAWGEALGIPAERMNSFDDKELWLRALQDAIGDRCML
                     VVLDDVWRTEDARRFMELGPRCVFLSTTRSKTVAKELGAGELVPELDPPQAFGLLCEV
                     APGAVEADPMAAKALLGTLQGLPLAIVLVGKVLRRAASEAHADPARLRRAYDEVAQAG
                     RRLGLLRPEDQRTLGEVIELSFGALRSDAARDAATALSIFRPKPNAFGRDIALAVGEA
                     DEQTLEDIGDIGLIEQVGKDVFTMHRIIAEYARTRLPLQRSQALHRKALAFYAEKLRG
                     SIESDPDAYLSWYRHEWAEWQETKDACLYHTAHSGDSVAGVLAFLRVFFDAFWWWGYY
                     QRFPFCERLVVEWRQRELDARARALIDQVAAFQDAYPAGPDKRAGGDWVRAESALLGI
                     RKALGLDGDGRHITGEDARRVRAFTDFFLAEARAYGRDERDEALRFYEAARSLFLDDG
                     VSWVAAWIEFYVAQYLLERGEFADARPHALRSLAEGAGEFGERAPLAGRDSELIANDY
                     RLLGDLALAADEVDAAARAHGRATLYAYVFQAIPEPVDTYTMDFYGEIVGRVAASIAR
                     CGSGDPRGRALALALHAFWRPWRTHCGIGDERDELNAALDAGDPGRLAAALFPPAPAV
                     DAFDFIDQAASYSRAVLAVVPSFVHELGPLDEFMAEVVRTLAGGAGADDAALAGHAPL
                     YRIDARKGRLQLCLGRTEFA"
     gene            complement(6180..6509)
                     /locus_tag="PKN25_04875"
     CDS             complement(6180..6509)
                     /locus_tag="PKN25_04875"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNV90266.1"
                     /translation="MVVIVFRTRLRAGVDEAELEGVGARMFQVAASMPGYVSYKDFAA
                     DDGEFVSIVEFDSLETLAAWRDHPEHREIQARGRSRYFSDYRVQVCTTVREYAFTLDG
                     GRVEGAL"
     gene            6738..8882
                     /locus_tag="PKN25_04880"
     CDS             6738..8882
                     /locus_tag="PKN25_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HNV90267.1"
                     /translation="MARSPSPQAEPAAFTPEQLRRHDLLLAGLDLLDQAIAVFDTTPK
                     LVTWNKALVRLLDFPEALVRVGTPFEAFVRFNAERGEYGEGDIETLVAKRMAAARAFL
                     PHYVERARPNGTVLAVRGVPIPNLGFVSLWTDITEQRRYEALIQQQNAQLEARVRERT
                     AELEQANSEIDQIAGALRRSEHRLQLIIDSIPALIAYVDGSEIYRFANRGYAEWFGYP
                     KDEIVGRSIREVVGEDAYAQIRSQVERARTGERVCYEYSRVGTDGRTLHARSVVVPEI
                     GTDGRTVVGFFVMTTDITEQKANQAALVQAQKMEAVGQLTGGLAHDFNNLLTIIIGNL
                     SALQQRIGAGPGAEFVDPSLQAARRGAELIRRLLTFSRRQSLAPTAVEVGALVHNMTH
                     LLARTLGETIRIHLRLPGEPVHALVDPHQLENAILNLAINARDAMPVGGELTIGVSRR
                     QLDEALAGVVEVAAGDYVQIDVGDTGKGIEPAVLQRVFEPFFTTKDFGSGSGLGLSMV
                     YGFVRQSGGNIRILSTPGRGTHVRFVLPTAAAAVTAPERAAPATARARALDGPVLLVE
                     DEPEVRKVVRMQLTSLGHVVIEAADGVEALGLLQNVDDIALLVSDTVMPGGIGGRELA
                     RRARTLRPQLPILLVTGYASEATPTDELPADVPTLRKPFDQAALAGALAALLGPEPGA
                     AAGVEDASPTAPAADGEARSRIMNTTTEPRPQ"
     gene            8879..9607
                     /locus_tag="PKN25_04885"
     CDS             8879..9607
                     /locus_tag="PKN25_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV90268.1"
                     /translation="MTQSPIDPSQARIFVLEDDPEVARLICMSLAEYGFRCEQMRAGR
                     QLLERARQLAPDLCIVDLGLPDMDGMQVVRELQDGSPCAVLILTGRNDVTDRVLGLEL
                     GADDYIVKPFEPRELVARVRSILRRYQRSASADSAGERSTARFAGWTFDANRHALLAP
                     DGREVPLSAAEAGLLATLLRRPNKILSREQLLGERDVDPFDRSIDVRISRLRRKLDDD
                     PQNPRLIKTVYGAGYLFAVQVEWV"
     gene            complement(9641..10396)
                     /gene="rlmB"
                     /locus_tag="PKN25_04890"
     CDS             complement(9641..10396)
                     /gene="rlmB"
                     /locus_tag="PKN25_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNV90269.1"
                     /translation="MAKAPENTPTRLIYGFHAVSAKLRHAPDSVLEIFLTADRKDARV
                     RKFLAQAESAGVRITQSEGDKLDALVGTRRHQGVVAKVDATRREIKLADVLDSLDENA
                     LILVLDGVQDPHNLGACLRVADAAGAHAVVAPKDRAVGLNATAVKVASGAADTVPYIT
                     VTNLARALREMQDAGVWVVGAAGEAEKTLYGVDQKVPVAWVLGAEGDGLRRLTRETCD
                     ELARIPMHGSVESLNVSVASGICLFEARRQRGS"
     gene            complement(10389..>10762)
                     /locus_tag="PKN25_04895"
     CDS             complement(10389..>10762)
                     /locus_tag="PKN25_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNV90270.1"
                     /translation="PKARGKRAKKVVAGQVAEVVAAPAIEPVPAAAEPVAPKARGKRA
                     KKAAIATVAEVVVAPVLEVTPDAAPPAAPKTRGKRAKKAVAGTATEVPAASAAEALES
                     APPVPARRAGRKTTTKGSDRG"
     assembly_gap    10763..10862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10863..13209)
                     /gene="rnr"
                     /locus_tag="PKN25_04900"
     CDS             complement(<10863..13209)
                     /gene="rnr"
                     /locus_tag="PKN25_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNV90271.1"
                     /translation="MQASAVRLADPFYERETQQYDNPLPSREYVLQILAERGVPMPLA
                     ELAGALDIAPHELDFFDRRLRAMERDGQVVRNRRDAYLLPAKADLIKGRVEGHPDGFG
                     FLRRDDGEPDVFLGPKEMREVLHGDRVMVRISGTDRRGRPEGKLVEVLERANSRVVGR
                     VINEHGVMIVVPENRRLAQDILVAPGGRKKPEPGQIVTVELVEQPTKFAQPIGRIVEV
                     LGNYADPGMEIEIALRKHDLPFEFSSEAKAQTRKLPDVVRKKDWAGREDLTKLPLVTI
                     DGETAKDFDDAVYCERQGKGYRLIVAIADVSHYVDAASALDKDAFDRGNSVYFPRRVI
                     PMLPEKLSNGLCSLNPQVERLAMVADMNIAATGEIRNYRFYPAVIWSHARLTYTKVAA
                     ALYDKDPAVRAELAALLPHLEQLDQLFRVLLKARAKRGAIDFETTETRMIFDDNGKIA
                     QIVPEVRNDAHRLIEECMLAANVCASDFLATREHPALYRVHDSPSEDKLAKLREFLKE
                     FGLGLGGGDEPRAADFARLLEQVKDRPDAQLLQTVMLRSLKQAMYSPDNVGHFGLAYE
                     SYTHFTSPIRRYPDLLIHRGIKAALAGEQYRPGDWEQIGLHCSMTERRADDATRDVVA
                     FLKCYFMQDRVGEEFVGSVSAVVPFGLFVALDDIFIEGLLHISDLGSDYFHYDETRHA
                     LMGERTGKQFRLSDRVKVQLVRVDMATNKIDFRLIEGPLPVEAKAPPKVAEVVAAEVV
                     PVEGKKARKPRSKKAEVEERVEATPAPVVEAVPAAAEPAAPK"
     gene            13489..13573
                     /locus_tag="PKN25_04905"
     tRNA            13489..13573
                     /locus_tag="PKN25_04905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13523..13525,aa:Leu,seq:cag)
     assembly_gap    13581..13680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14017..15327)
                     /locus_tag="PKN25_04910"
     CDS             complement(14017..15327)
                     /locus_tag="PKN25_04910"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNV90272.1"
                     /translation="MAKNVVVVGTQWGDEGKGKIVDWLTDHAKGVVRFQGGHNAGHTL
                     VVGKTEYKLNLVPSGIVREGVACFIGNGVVLDAHHLLSEIRTLEAGGIKVRDRLRVSP
                     GCPLILGYHAALDRAREAAKSAGDKIGTTGKGIGPTYEDKVARRALRVYDLFDRERFA
                     AKLKANLEYHNFVLTRHLGAEPVDFQTVFDQAMADADELLPMVADVSAELYAINKSGG
                     SLLFEGAQGTLLDIDHGTYPFVTSSNCVAGQAAAGSGVGPGRLHYVLGITKAYCTRVG
                     GGPFPTELDIETKGVPGEQMSTKGREFGTVTGRKRRCGWLDLAALKRSIIINGVTGLC
                     ITKLDVLDGLEELKLCTGYMLDGRRIDLLPMGSEEVTRCEPIYETLKGWSGTTFGAQS
                     WDALPQEARTYLHRIEEICEIPIDVISTGPERDETILRRHPFGA"
     gene            complement(15332..16489)
                     /locus_tag="PKN25_04915"
     CDS             complement(15332..16489)
                     /locus_tag="PKN25_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNV90273.1"
                     /translation="MRWVLPDHIQDALPSDAHRLESLRRRLLDAFRLRGYQLVMPPLL
                     EYLDSLTTGAGKDLELRTFKLVDQLSGRSMGVRADMTPQVTRIDAHLLNRRGVSRLCY
                     CGSVLHTLPSTLTGTREPLQLGAELYGHAGIEADVEILRLLAEVLRLAEVPASRIDIG
                     HVGLFHALAARAGLVPGREEELFDLLQAKDVPELRRMLADVSEPARSALLALPELYGG
                     PEVLAEAAARLPQDAEIAGPLAELRQLADALGDLPVSFDLADLRGYHYHSGVVFAAYG
                     AESPAALALGGRYDRVGQAFGRARPATGFSLDLRELAWHLPIPAAPAGAVLAPPDDDA
                     GLASEVSALRARGEIVMVALPGHEGTWNEAGCDRQLVKRGDRWAVVPLQGE"
     gene            complement(16497..16682)
                     /locus_tag="PKN25_04920"
     CDS             complement(16497..16682)
                     /locus_tag="PKN25_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HNV90274.1"
                     /translation="MASSLLTAFALMLIVEGLLPFIAPAAWRETFLRLASMADGQIRF
                     IGLSSMLAGLAMLFIVL"
     gene            complement(16690..17571)
                     /gene="hflC"
                     /locus_tag="PKN25_04925"
     CDS             complement(16690..17571)
                     /gene="hflC"
                     /locus_tag="PKN25_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNV90275.1"
                     /translation="MRDKMSLIGGTLLLLVVIASMSLFTVDQRQYAIVFQLGEVKEVI
                     SEPGLNAKLPFIQNVRYFDKRILTMDTPEPERFITSEKKNVLVDHFVKWRIVDPRLYY
                     ESVAGDEARARTRLTQTVNAGLREEFGRRTVHDVVSGERDRIMEQMRERADRDARTIG
                     VQIVDVRLKRVDLPNEVSESVYRRMEAERKRVANELRSLGAAEAERIRADADRQREVI
                     IAEAYRSAQEVKGAGDAKATAIYAEAFGKDREFYSFYRSLEAYRASFSGKDDVLVVDP
                     SSDFFRFMKDAGGAPRN"
     gene            complement(17571..18872)
                     /gene="hflK"
                     /locus_tag="PKN25_04930"
     CDS             complement(17571..18872)
                     /gene="hflK"
                     /locus_tag="PKN25_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNV90276.1"
                     /translation="MSLNDPRWGGQGGGDREDGNRGDDNRNDDQRGDRNRGGNQGPPD
                     LEEVWRDFNQRLSGMFGGKRPGRGGGFGGGNGGGNRPELPGFSFKQFRGGFGVLAALV
                     LVVWLASGLYTVDANQRAVVLRLGEYVATTEPGLRWRLPAPFETHEIVDLTGVRTVEV
                     GYRGSERNKVLRESLMLTDDENIINIQFAVQYVLNSPENYIFNNRFPDEAVAQAAETA
                     MREIVGKSRMDFVLYEGREEIATTAHELMQRILDRYETGIQVSRVTMQNAQPPEQVQA
                     AFDDAVKAGQDRERQKNEGEAYANDVVPRARGTASRLVEEANAYRERVVANAEGEASR
                     FSQVFAEYNRAPEVTRERLYLDTMQQVMSSTSKVMVDAKGNGNLLMLPLDKLMQQTGA
                     KPAAAAPPDSQSAAGLAANAPVPSFDPRNRDGMFSRERGVR"
     gene            complement(18887..20044)
                     /gene="hflX"
                     /locus_tag="PKN25_04935"
     CDS             complement(18887..20044)
                     /gene="hflX"
                     /locus_tag="PKN25_04935"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNV90277.1"
                     /translation="MFERPASGERAVLVQLDLGQGAIDERLSELKLLVLSAGASVEAV
                     VQGRRAAPDSKLFAGSGKVQEIGEALRAYGADIVIFNHALSPGQQRNLERELQCMVID
                     RTALILDIFAQRARSHEGKLQVELAQLEHLATRLVRGWTHLERQKGGIGLRGPGEKQL
                     ETDRRLLGNRVKMLKSRLAQIEKQRKVRRRARERRDVLSVSLVGYTNAGKSTLFNALT
                     KAGAYAADQLFATLDTTSRRLYVGGEGAGASVVLSDTVGFIRDLPHALVAAFQATLEE
                     TAQADLLLHVVDSASEDRDAQIGAVNQVLAEIGAADVPQILVWNKIDLTRAAAAVERG
                     DCGTIRRIFLSARTGEGLDLLRDALAEVARQTFGDNADRPAGRDDERRQET"
     gene            complement(20099..20335)
                     /gene="hfq"
                     /locus_tag="PKN25_04940"
     CDS             complement(20099..20335)
                     /gene="hfq"
                     /locus_tag="PKN25_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNV90278.1"
                     /translation="MSNKGQLLQDPFLNTLRREHIPVSIYLVNGIKLQGQIESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVVIQQEEGAN"
     gene            complement(20450..21778)
                     /gene="der"
                     /locus_tag="PKN25_04945"
     CDS             complement(20450..21778)
                     /gene="der"
                     /locus_tag="PKN25_04945"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNV90279.1"
                     /translation="MKPTIVLVGRPNVGKSTLFNRLTRTRDALVADQPGLTRDRHYGI
                     GRVGDRDYLVVDTAGFDPVAKDGIMHEMARQAEQAIAEADVLLFLVDGRAGRTPHDEN
                     IAARLRRAGRPVHVVVNKAEGIDRAMVAADFHALGLGDPLPVSAAHGDGVKQLVELVL
                     APFPADDEKEHSDDEGPKVAIVGRPNVGKSTLVNTLLGEERVIAFDMPGTTRDAIAIP
                     FERGGKRYTLIDTAGLRRRGKVFEAVEKFSVIKTLQAVQECNVVVLVLDAAQDISDQD
                     AHIAGFALEAGRALVVAINKWDAVDDYRRDRLKADIARKLAFLSFARFHQISALKAEG
                     IAGLLKSVDAAYAAATANLATPRLTRALQQAVARQAPPRHGLARPKMRYAHQGGMNPP
                     VIVIHGNALDDIPQSYVRYLERCFMDAFDLKGTPLRIQFRTTHNPYATRT"
     gene            complement(21792..22934)
                     /gene="bamB"
                     /locus_tag="PKN25_04950"
     CDS             complement(21792..22934)
                     /gene="bamB"
                     /locus_tag="PKN25_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HNV90280.1"
                     /translation="MKTPSLRLVPLLVAALLAAGCSSLNPFASSAPKPAPLVDFKPGA
                     QLVAKWQANIGEAEHYRFQPAVWSDSVYAAGNEGEVARFEDGRTVWRIDTGGELSSGV
                     GTDGRLAVVVATDGTVIALDTDNGSERWRAPIGAEVLAPPAVSLELVIVRASDNRLIA
                     LSARDGSRRWVYQRNNPPLALRSFAGVVLEGPVVLAGFPGGKLAVINAANGGAITELN
                     VAVPRGATELERVADVAGMPVVGRREVCAVAYQGRAACFDTSNGNAIWSREFSSSVGM
                     DRDARFALITDDRDAVHALDAASGASVWKQDALARRHVSRPLIVGDYVIVGDLEGYVH
                     ALSRETGAFAARERAGSDPIVADPVPLRRGFVVQGAGGDITAYEVR"
     gene            complement(22938..23582)
                     /locus_tag="PKN25_04955"
     CDS             complement(22938..23582)
                     /locus_tag="PKN25_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV90281.1"
                     /translation="MAVYDLEEQEQISELKAWWAQYGNFVVTLAVVAALASVGWQAWN
                     WYQSRNAGEAGALYYAVQQAVEKNEPQKAREAAGRLVSEHGGSVGAQLGALLSAAMQF
                     ESGDLANARAQLEWAADKGSDPALRELARLRLAAVLLQAGELDAALARLQSAPSAAYK
                     ARFDDLRGDVLAAQGKVAEARAAYQAAIDALAAVGDDAVTLREVVRVKLESLGA"
     gene            complement(23585..24880)
                     /gene="hisS"
                     /locus_tag="PKN25_04960"
     CDS             complement(23585..24880)
                     /gene="hisS"
                     /locus_tag="PKN25_04960"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNV90282.1"
                     /translation="MSQTLQAVRGMNDILPADAETWEYFEDIVRDWLQSYGYRPIRMP
                     LVEPTPLFKRAIGEVTDIVEKEMYSFEDALNGEHLTLRPEGTASCVRAAIQHNLIPAG
                     GPQRLYYYGPMFRHERPQKGRYRQFHQIGVEALGFAGADTDAELILMCARLWEDLGLE
                     DVALEINSLGSPEERAQHRAALIAHLEQHQDKLDEDGKRRLYTNPLRILDTKNPELQA
                     IVEAAPRLADYLGDESKAHFEAVQVFLKDAGIPYRINHRLVRGLDYYNRTVFEWVTTR
                     LGAQGTICAGGRYDGLFEQLGGKPQPAAGFAIGIERLLLLWQACGGEAERPVPDVYVV
                     SVGEAAQRLGFRAAETLREHGFAVLMHCGGGSFKSQMKKADASEAPVAIVIGEDEAAA
                     GEVGLKPLRVAGAQQRVAIDDLVEAMAALMFPEEEDEEV"
     gene            complement(24938..26197)
                     /gene="ispG"
                     /locus_tag="PKN25_04965"
                     /gene_synonym="gcpE"
     CDS             complement(24938..26197)
                     /gene="ispG"
                     /locus_tag="PKN25_04965"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNV90283.1"
                     /translation="MNPRHELQPIEARPLARHRTHQVRVGKVRIGGEAPVVVQSMTNT
                     DTADVLATAMQVAELARAGSEIVRITVNNEAAAAAVPKIRDRLLALNMDVPLVGDFHY
                     NGHKLLTDFPACAEALAKLRINPGNVGAGRKRDPQFAAIVELACRYDKPVRIGVNWGS
                     LDQSVLARIMDANAKRAEPRDAGAVMREALVVSALESAAKAEEYGLGRERIILSAKVS
                     SVQDLIAVYRDLARRSDYALHLGLTEAGMGSKGIVGSTAALAVLLQEGIGDTIRISLT
                     PEPGGSRTQEVVVAQEILQTMGLRAFTPMVTACPGCGRTTSTFFQELASGIQDYVRAQ
                     MPVWREQYDGVENMTLAVMGCVVNGPGESKHANIGISLPGTGETPAAPVFVDGEKVVT
                     LRGDNIAAEFKALVDDYVATRYVKKGA"
     gene            complement(26208..27176)
                     /locus_tag="PKN25_04970"
     CDS             complement(26208..27176)
                     /locus_tag="PKN25_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNV90284.1"
                     /translation="MQSDHLALGAESAASPGARLRRAREARGESVHEAAFAIKLSPRQ
                     IEALENDDFAALPGMAFVRGFARNYARYLGLDATPLLDGIERLAGAGQPDLSPIRNAD
                     GDLPAGGGGRRGSFPAGVVVALLLVLLGVGWYFDWFRTEPLPGLELQTEPAPAFMPAP
                     TEGGGAAPATSEPVATPAPLEASAPAAPHASPVAIPAAVGAGVPATPLAAPASAMPPA
                     SASAIVSPVVQSPAQAATPVAEEGAGGNRLSFRFGGDSWIEVRDASGTILHSGTNRAG
                     SSRVVQGTPPFRLVVGNSANVVLEQDGKAVDLAAHTRGSVARLTLP"
     gene            complement(27182..27982)
                     /gene="pilW"
                     /locus_tag="PKN25_04975"
     CDS             complement(27182..27982)
                     /gene="pilW"
                     /locus_tag="PKN25_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HNV90285.1"
                     /translation="MKSTLAPVVLAVALVLGGCAGAPGVPAGSSSISRPMSDTNPATP
                     ADARARVHVELGMAYFEVGRYDVALDEAKIALADSPSYAPAYHLLGLAYMSVEEHAAA
                     RENFERALREAPGDPDFNNSYGWFLCSQGQERAGLEYLALAARNPYYRHQTRPLTNAG
                     LCHLRLGEEAAAEAQFQRAVQADPENAQALYLLADIAYRGGRYEQARSELIRLHQAHG
                     PSAASAWLGLRTERRLGNADAEASYAAQLRSRFAESEEFKSMNQGKFE"
     gene            complement(27979..29127)
                     /gene="rlmN"
                     /locus_tag="PKN25_04980"
     CDS             complement(27979..29127)
                     /gene="rlmN"
                     /locus_tag="PKN25_04980"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNV90286.1"
                     /translation="MSTPNPVNLLDFDVDGLVAWFAGLGEKPFRARQVMRWMHHEGCD
                     DFDAMTDVAKSLRAKLKDLAVIRPPVPVRDSISADGTRKWLLDVGNANAVETVFIPET
                     SRGTLCVSSQAGCALDCAFCSTGKQGFNRNLSAAEIIGQLWLANKLLGAARADAEEHA
                     TDLEAGEKDNGRIISNVVMMGMGEPLANFDNVVTALRLMLDDHAYGLSRRRVTVSTSG
                     IVPAMDRLRDECPVALAVSLHASNDALRDRLVPINQKYPLRELMAACQRYLERAPRDF
                     VTFEYVMLEGVNDSDAHARELVALVRDTPCKFNLIPFNPFPDSGFQRSPAERIRRFAG
                     ILIDAGIVTTTRKTRGDDVDAACGQLAGQVQDRTRRTVRLHQARENMR"
     gene            complement(29260..>29552)
                     /locus_tag="PKN25_04985"
     CDS             complement(29260..>29552)
                     /locus_tag="PKN25_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNV90287.1"
                     /translation="EAGQFYAVHKERPFFKDLVSFMTSGPVMIQCLEGENAIAKNREL
                     MGATDPKKADKGTIRADFAESIDANAVHGSDAPETAAVEVAFFFPGMNVYSR"
BASE COUNT         4318 a         9984 c        10414 g         4636 t
ORIGIN      
        1 ccctcgcgct tgaacgcgaa tacgccggcg gtgtagggga aggagccggg aacgttctcc
       61 ttcatcagga acttgagcgt ctcgcccgcg tcctcgaagt tgggcagcgc caccttgcgg
      121 atcttgctgc ccgacagcga ggtgctggtg agctgggtgc ggatctcctt gtcgcggatc
      181 ttcaccacgt actcgtcggc ggcgtagagc tcgcgcgtct tcggccacat gtcgagcagc
      241 ttcttcgcgg ccggggtgag ctcgccgtcc ttccagtcga tgagttccgc gaacgagccc
      301 gcgtccttgc cgcactgctc gaagaggccc ttggcgatct tcagcgactg gcgctcgcgc
      361 gcgacgcgag cctgctgctc gatgtgcttg tggtagccgc gcacggtgtc ggcgatctcg
      421 gcgaggtagc gcgtgcggtc ggcggggacg atggcgcgac cttcggacga ggcgcgcacc
      481 ttgaccaccg gcagcttgct cttggcgacc ttgaggccct tgccgacgag cgccggcagc
      541 acggcctggt agagcgcggt gacgccgtcg tcgttgaagc gcgcggccat ggtgccgaac
      601 accggcatct cttcggggcg ctggttgaac agctcgcggt tgcgctggta ctgcttggca
      661 acgtcgcgca gcgcgtccag cgcgcccttg cggtcgaact tgttgatggc gacgaagtcg
      721 gcgaagtcga gcatgtcgat cttctcgagc tggctggcgg cgccgaactc gggggtcatc
      781 acgtacagcg acagatcgac gaagggcacg atcgcggcgt tgccctggcc gatgccggag
      841 gtctcgacga tgacgaggtc gaagcccgcg agcttgcacg cggcgatgac ttcgggcagc
      901 gcggcggaga cttccgagcc ggtgtcgcgg gtggcgagcg agcgcatgta gatgttggcg
      961 tgctcgatcg cgttcatgcg gatgcggtcg ccgagcagcg cgccgccggt gcgcttgcgc
     1021 gaggggtcga tggagacgat ggcgagcttg agcttgtcgt cctggtcgag gcggaagcgg
     1081 cgcaccagct cgtcggtcag cgaggacttg cccgcgccgc cggtgccggt gatgccgagc
     1141 gtcggcacct tggtcttggc cgcggcctcg atcagcgcct tcttgacctc gtcgccgtag
     1201 ccgccgttct ccagcgcggt gatgatctgc gcgagcgcgc ggcgatcgcc ggcggcgagc
     1261 gccttgagca ccgcatcggc cttctgcggc gcggccttgg cgacgtcgac gtcgcagcgc
     1321 tcgacgacgc tgttgatcat gccctgaagg cccatcttgg cgccgtcttc gggcgagaag
     1381 atgtgggtga cgccgtactc gtggagttcc ttgatctcgg ccggcacgat cacgccgcca
     1441 ccgccgccga agaccttgat gttctcgccg ccgttggcgc gcagcaggtc gatcatgtac
     1501 ttgaagaact cgacgtgacc accctggtag gaggtgatcg cgatgccctg cacgtcctcc
     1561 tgcagcgccg cattgacgat ctcgccgacc gagcggttgt gaccgaggtg gatgacctcg
     1621 gagccggtcg actgcaggat gcggcgcatg atgttgatgg acgcgtcatg gccgtcgaac
     1681 agcgcggcag cggtgacgaa acgcaccttg ttcttcggct tgtagggcga cagcttgtgc
     1741 gcaacgctca gatcggtcat gtcgttcgtc tccaggtggg tgtgaacatc cctgcaatat
     1801 tagaagggtc ttcacgcctt tgccattgcg cttgccgacg aggatcgtgc aaaatccgcc
     1861 aaatcgagca accgatggcg cgctcggcgg cttgccggtt gccgctttcc cgtgggagcg
     1921 acgcaagtcg cgattgcagc gctaccgcgg acgatgacga cggcaggcac cggatgcgga
     1981 aatcgcaacg tgtgccgcgc ctgcacagcg cagcgtcgcg aagcctctgc gcagcgccgg
     2041 ctcgatccct ccacccaagc caggagcccg ccctgtcgac tcccagcctt tccgcttccg
     2101 cgatcccccg ccgtagccgc gccacggtcg cgcccggctt cgtcaccggc atgctggccg
     2161 ggctgcagcg gcgcggactc gatggggcgc cgctgctcgc acgcgccggg atcgaccttg
     2221 cagaaaccga cacgcgcatc ccggtcgagc gctacgccct gctctacaac ctgtgcgtgg
     2281 aggcacttga agacgaggcc ttcggcctgc tcccggagcc gatgcgcccg ggcagcttcg
     2341 agttcctctg ccgcgcgctg ctcggcgcgc ccacgctggg cgaggccctg ctgcgcgcga
     2401 tccgcttcct gcgcatcgtg ttgcccgcct tccgcatcga gctgcgggtg gacggcgcgc
     2461 gcgccgagtt gctgctggac gacggcggca gcctcggccc cgggctcgac gccccggcgc
     2521 gcgtgttcgc ctacgaatgg ctgctgcgcc tgctgcacgg cgtggcgagc tggttcgtcg
     2581 gccgcggcct ggcgctcgac gcggtcgcct tcccgtacgc acgcccggcc catgcggacg
     2641 actacgcgct cgtctacacc gagcattcga gcttcgacgc gccgcagctg gccgcccgcc
     2701 tgcaggccaa cctgctggcg ctgccgctgc gccgcgacga ggcggcgctg gtcggcttcc
     2761 tcgagggcgc accgggaaag atcaccaccc tctaccggcg cgaccgcgag atggtcttcc
     2821 gcgtgcgcga catcctgcgc gacgcgctgc cgcagaacct ttcgctcgaa gaggtcgccg
     2881 agcgcctgca cgtgtcgccg cgcaccctgc accggcggct ggaggatgag ggctcgggat
     2941 tccgcaacat caaggaggcc acccgccgcg acatcgccta tgcgcgcctg gccaagaccc
     3001 gccagcccat cgcccgcatc gcggccgagc tcggctacgc cgacccgtcc accttctacc
     3061 gcgccttcgt cgcctggagc ggcatgtcgc cggagcagtt ccggcaccgg ctggcgggca
     3121 acgacagtct tcccgcggcg tctgccggtc cggacaggcg ccctgcccca accccccgtg
     3181 tcaccgccgg acggcagcga tcacgcgaat tcggtccgac cgaggcatag ctggagtcgc
     3241 cccttgcggg cgtcgatgcg atacaggggc gcatgcccag caagcgctgc gtcgtccgct
     3301 cccgctcccc ccgccagggt cctgaccacc tccgccatga actcgtcgag cgggcccagc
     3361 tcgtgcacga acgagggcac caccgcgagc acggcgcgac tgtaggatgc cgcctggtcg
     3421 atgaagtcga acgcgtccac cgccggggcg ggcgggaaca gcgcagcggc caggcgcccc
     3481 gggtcgccgg catccagcgc tgcattcagt tcgtcgcgtt cgtcgccgat gccgcaatgc
     3541 gtccgccaag gccgccagaa ggcgtggagc gcaagcgcca gcgcgcggcc gcgcgggtca
     3601 cccgatccgc agcgcgcgat cgatgcggcg acacgaccga cgatctcgcc atagaagtcc
     3661 atcgtgtagg tgtccaccgg ctcggggatc gcctggaaga cgtaggcgta gagcgtggcc
     3721 cgaccatggg cacgggcagc cgcgtcgacc tcgtcggcag ccagcgcgag atcgccgagc
     3781 aggcgatagt cgttggcgat gagttcggag tcgcgccccg cgagcggcgc ccgctcgcca
     3841 aactcgccgg cgccctcggc cagggaacgc agggcatgcg ggcgggcatc ggcgaactcg
     3901 ccgcgctcga gcaggtattg ggccacatag aactcgatcc aggccgcgac ccaggacacg
     3961 ccgtcgtcga gaaacagcga gcgcgccgcc tcgtagaagc gcaaggcctc gtcgcgctcg
     4021 tcacgcccat aggcccgtgc ttcggcaagg aagaaatcgg tgaaggcacg cacccgtcga
     4081 gcatcctcgc cggtgatgtg gcggccatcg ccatcgaggc cgagcgcctt gcgtatcccg
     4141 agcagcgcgc tctcggcgcg cacccagtcg ccgccggcac gcttgtcggg gcccgccggg
     4201 taggcatcct ggaacgctgc cacctgatcg atcaaggcac gcgcccgcgc gtcgagttcg
     4261 cgctgcctcc actcgacgac cagccgctcg cagaacggga agcgctggta gtagccccac
     4321 caccagaacg catcgaagaa cacgcgcagg aaagccagca cgcccgccac cgagtcgccg
     4381 ctgtgggcgg tgtggtacag gcaggcgtcc ttggtctcct gccactccgc ccattcgtgg
     4441 cgataccagc tcagataggc atccggatcg ctctcgatcg agccccgcag cttctcggca
     4501 tagaaggcga gcgccttgcg atgcagcgcc tgcgagcgct gcaggggcaa gcgggtgcgc
     4561 gcgtactcgg cgatgatgcg gtgcatcgtg aacacgtcct tgcccacctg ctcgatcagc
     4621 ccgatgtcgc cgatgtcctc cagtgtctgt tcgtcggcct cccccacggc gagcgcgatg
     4681 tcgcggccga aggcgttcgg cttgggacgg aagatggaca gcgcggtcgc cgcgtcgcgc
     4741 gccgcgtcgc tgcgcagcgc gccgaaggac agttcgatca cctcccccag cgtgcgctgg
     4801 tcttcaggac gcagcagccc gagccggcgg cccgcctgcg ccacctcgtc gtaggcgcgg
     4861 cgcaggcgtg cgggatcggc atgcgcttcg ctcgccgccc ggcgcaagac cttgcccacc
     4921 agcacgatcg ccagcggcag gccctgcagg gttccgagca gggccttggc ggccatcgga
     4981 tccgcctcga cggcgcccgg ggcgacctcg cacaggaggc cgaaggcctg cggcgggtcc
     5041 agctccggga ccagctcgcc ggcgccgagc tccttcgcca ccgtcttcga gcgcgtggtg
     5101 gacaggaaga cacagcgcgg acccagctcc atgaaacgcc ttgcatcctc ggtgcgccac
     5161 acgtcgtcga gcaccacgag catgcagcgg tcgccgatgg cgtcctgcag cgcacgcagc
     5221 caaagctcct tgtcgtcgaa actgttcatg cgctcggccg ggatgccgag cgcttcgccc
     5281 catgcacgca gctccgcgct gtcgacgtgt tgccggccga gattggccca caagacgccg
     5341 tcgaaatgcc cgagtatctt gtcgcgctcg cgcagcagtt cctgcgcgac cgtggtcttg
     5401 ccgacgccgg gcaggaacag caggctggtg tcgacgccgt cccgcagctt gcgccacaca
     5461 cggtcgacga gctccgcgcg ccccaccacg tggtaatcgc gcacgggctc gcgcaccagc
     5521 cagggcttcg cccctgcgcc cgccccgccc gcgggcgcac gcggactcgc gccccgcagg
     5581 gcatcgatgc gccgcgtgat gaaggcctcg aggtgctctc cgagcgcctt cccgaagctg
     5641 gtcggcgtat cgaaggtgtt cacgccgccc ttgagcgaac catcgggatt ctggaatgcc
     5701 cgcacgaagt aagcttccac cgccctgtac tgcgcgagct tttcgtcgac gcgttgggcc
     5761 ttgaggtcat cacccgagat ctcgagcgga cgggtgcagc ggtagaccca cacatcgcgg
     5821 ccgcgatccc ttgcgtcctc gagttcccag acggttcccg actcgaacgc actgccgtcg
     5881 gcacggcgga acgagttcgg caggcgcgtg ccgatgcggg accacaggat gaccaccgtg
     5941 agctcgcaat cccgcggccg gcccaggtag tcgatgaccg aatcctccgg cgtcgccgac
     6001 gccgccatcg gcacctgggc aaggtcgtcg tcccattcga cggtgtcgag cgtcaccttt
     6061 ccgcgcgcga accagcgccg ctgcatgccg ttgatgacct cgagcgccag cttgcgctcg
     6121 gccgcgacat cacctggcga ggcgacgaag acgcgcagat tctcgggcgg cgccggcatt
     6181 cacaaagccc cttcgacccg cccgccgtcc agcgtgaagg cgtactcgcg cacggtcgtg
     6241 cacacctgta cccggtaatc gctgaagtac cgactgcggc cccgcgcctg gatctcccgg
     6301 tgctcggggt ggtcgcgcca tgcggcgagg gtctccagcg agtcgaactc gacgatggaa
     6361 acgaactcgc catcgtccgc cgcgaaatcc ttgtacgaga cgtagcccgg catggatgcg
     6421 gccacctgga acatgcgggc gcccacacct tcgagttccg cctcgtcgac gcccgcgcgc
     6481 aggcgcgtgc ggaatacgat cacgaccatg acgtcctccc gcgcttccgg ttcgcatccg
     6541 cgcgccgccc gccggcgaat cggatgcatt cccatgatag tcaagctttc gcgcccgcgc
     6601 cgaggagagg ccgcccgaca caaggtcgcg gagccttgtc gcccaccgcg gtgaacgcca
     6661 caggcatcgc gacgcgagat gtgcaaacat cgcgctcatg atcctcgaaa ctcgccacac
     6721 cggagactgc cgcgagcatg gcaagatcgc cctcccctca ggctgaaccc gccgccttca
     6781 ctccggagca gctccggcgg cacgacctgc tgctggccgg actcgatctg ctcgaccagg
     6841 ccatcgcggt gttcgacacc acgcccaagc tcgtcacctg gaacaaggcc ctggtgcgcc
     6901 tgctcgattt ccctgaggcg ctggtgcgcg tcggcacccc gttcgaggcc ttcgtgcgct
     6961 tcaacgccga acgcggcgaa tacggcgaag gcgacatcga aaccctggtc gcgaagcgca
     7021 tggcggcggc acgggccttc ctgccgcact acgtcgaacg cgcacggccc aacggcacgg
     7081 tgctggcggt acgcggggtg ccgattccca acctcggttt cgtgtcgcta tggaccgaca
     7141 tcaccgagca gcgtcgctac gaggcgctga tccagcagca gaacgcgcag ctcgaagcgc
     7201 gcgtgcgcga gcgcacggcg gagctggagc aggccaacag cgagatcgac cagatcgccg
     7261 gcgcgctgcg tcgcagcgaa caccgcctgc aactgatcat cgactccatc cccgcgctga
     7321 tcgcgtatgt ggacggcagc gagatctacc gcttcgccaa ccgcggctac gccgaatggt
     7381 tcggctatcc gaaggacgag atcgtcggcc gcagcattcg cgaggtggtc ggcgaggacg
     7441 cctacgccca gatccgctcg caggtcgagc gcgcgcgcac cggcgagcgt gtgtgctacg
     7501 aatacagtcg cgtcgggacc gacggtcgca cgctgcatgc acgcagcgtg gtggtgcccg
     7561 agatcggcac cgacgggcgc acggtggtgg gcttcttcgt catgaccacc gacatcacgg
     7621 aacagaaggc caaccaggcc gcgctggtgc aggcgcagaa gatggaggcg gtgggccagc
     7681 tcaccggcgg gctggcgcac gacttcaaca acctgctcac catcatcatc ggcaacctct
     7741 ccgcactgca gcagcgcatc ggcgccgggc cgggggcgga gttcgtcgat ccctcgctgc
     7801 aggcggcacg gcgcggcgcc gagctgatcc gccgcctgct caccttctcg cgccggcagt
     7861 cgctcgcccc caccgcggtc gaggtcggcg cgctcgtgca caacatgacg cacctgctcg
     7921 cgcgcaccct gggcgagacc atccgcatcc atctgcgcct gcccggcgag ccggtgcacg
     7981 cgctggtcga cccgcaccag ctcgaaaacg cgatcctcaa cctcgcgatc aacgcacgcg
     8041 acgccatgcc cgtgggcggc gagctgacca tcggcgtgag tcgccgccag ctcgacgagg
     8101 ccctcgcagg ggtggtcgag gtggcggccg gcgactacgt gcagatcgac gtcggcgata
     8161 ccggcaaggg catcgagccg gccgtgctgc agcgcgtgtt cgagcccttc ttcaccacca
     8221 aggacttcgg cagcggcagc gggctgggcc tgtcgatggt gtatggcttc gtacgccagt
     8281 cgggcggcaa catccgcatc ctcagcacgc cggggcgcgg cacgcacgtg cgcttcgtgc
     8341 tgcccacggc ggcggcggcc gtgactgccc ccgagcgcgc agcgcccgcg acggcccgcg
     8401 cgcgcgcgct cgacggcccg gtgctgctgg tggaggacga gcccgaggtg cgcaaggtgg
     8461 tgcgcatgca gctcaccagc ctgggccacg tcgtgatcga ggccgccgac ggcgtcgagg
     8521 cgctcgggct gctgcagaac gtcgacgaca tcgcgctgct ggtgagcgac acggtgatgc
     8581 cgggcggcat cggcggccgc gagctcgccc gccgcgcgcg cacgctgcgc ccgcagctgc
     8641 cgatcctgct ggtgaccggc tacgccagcg aggcgacgcc gaccgacgag ctccccgccg
     8701 acgtgccgac gctgcgcaag cccttcgatc aggccgcgct ggcgggcgcg ctggcggccc
     8761 tgctcgggcc cgaaccgggg gcggcggcag gggtcgaaga cgcgtcgccg actgcgccag
     8821 ccgccgacgg agaagcacga tcccgcatca tgaacaccac cacggagcct cgcccccaat
     8881 gacccagagc ccgatcgacc cctcccaggc ccggatcttc gtgctcgagg acgaccccga
     8941 ggtcgcccgc ctgatctgca tgagcctggc cgaatacggc ttccgctgcg aacagatgcg
     9001 cgccggccgt cagctgctcg agcgcgcgcg ccagctcgcc cccgacctct gcatcgtgga
     9061 cctcggcctg cccgacatgg acggcatgca ggtggtgcgc gagctgcagg acggcagccc
     9121 gtgcgcagtg ctgatcctga ccggccgcaa cgacgtgacc gaccgcgtgc tcggtctcga
     9181 gctcggcgcc gacgactaca tcgtcaagcc cttcgagccg cgcgagctgg tcgcccgcgt
     9241 acgctccatc ctgcgccgct accagcgctc cgccagcgcc gacagcgccg gcgaacgcag
     9301 cacggcgcgc ttcgccggct ggaccttcga cgccaaccgc cacgccctcc tcgcccccga
     9361 cggccgcgag gtcccgcttt ccgcagccga ggccggcctc ctcgccaccc tgctgcgtcg
     9421 ccccaacaag atcctgtcgc gcgaacagct cctcggcgaa cgcgacgtcg atcccttcga
     9481 ccgcagcatc gacgtccgca tctcgcgcct gcgccgcaag ctcgacgacg acccgcagaa
     9541 cccccgcctg atcaagacgg tgtatggcgc ggggtatctg tttgcggtgc aggtggagtg
     9601 ggtgtgagag gccaaggggc aggccgggac caccacagct tcaggagccg cgctggcgcc
     9661 tggcctcgaa caggcagatg ccgctggcga cggacacgtt gaggctctcg acgctgccgt
     9721 gcatcgggat gcgcgcgagc tcgtcgcagg tctcgcgggt gaggcggcgc aggccgtcgc
     9781 cctcggcgcc gagcacccag gcgacgggga ccttctggtc gaccccgtag agggtcttct
     9841 cggcctcgcc ggcggcgccg acgacccaca cgccggcgtc ctgcatctcg cgcagcgcgc
     9901 gcgcgagatt ggtgacggta atgtagggca cggtgtcggc ggcgccgctg gcgaccttga
     9961 cggcggtggc gttgagaccc accgcgcgat ccttgggcgc gaccacggcg tgggcgccgg
    10021 cagcatcggc cacgcgcagg caggcgccga ggttgtgcgg atcctgcacg ccgtcgagga
    10081 cgaggatgag cgcgttctcg tcgaggctgt cgagcacgtc ggcgagcttg atctcgcgcc
    10141 gggtggcgtc gaccttggcg accaccccct ggtggcggcg ggtcccgacg agggcgtcga
    10201 gcttgtcgcc ctcgctctgg gtgatgcgta cgccggcact ctcggcctgg gcgaggaact
    10261 tgcgcacgcg cgcgtccttg cgatcggcgg tgaggaagat ctcgagcacc gagtcgggcg
    10321 cgtggcgcag cttggcggag acggcgtgga agccgtagat cagccgggtg ggtgtgtttt
    10381 cgggtgcttt agccacggtc ggagcccttt gtggtggtct tgcggccggc acgtcgggcc
    10441 ggtaccgggg gcgcggattc tagcgcctcg gcggcggacg cggcggggac ttcggtcgcg
    10501 gtgccggcga cggccttctt cgcgcgcttg ccgcgtgtct tgggcgcggc gggcggagcc
    10561 gcatccggcg taacctcgag caccggtgcg acgacgacct cggcgaccgt tgcgatcgca
    10621 gccttcttcg cacgcttgcc gcgcgccttg ggcgcgacag gttccgcggc ggcgggcaca
    10681 ggctcgatcg ccggcgcagc cacgacctcg gccacctgcc cggcgaccac cttcttcgcg
    10741 cgcttgccgc gcgccttggg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnccttgggc gcggcgggct ccgctgcggc aggcacagcc tcgaccaccg gcgcaggtgt
    10921 ggcctcgacc ctctcctcca cctcagcctt cttgctgcgc ggcttgcgcg ccttcttccc
    10981 ttccacgggc acgacctccg cggccaccac ttcggcgacc ttcggcgggg ctttggcctc
    11041 gaccgggagc ggcccctcga tgaggcggaa gtcgatcttg ttggtggcca tatccacccg
    11101 cacgagctgc accttgaccc ggtcggacag gcggaactgc ttgccggtgc gctcacccat
    11161 gagggcatgg cgggtctcgt cgtagtggaa gtaatcgctg ccgaggtcgg agatgtgcag
    11221 cagcccctcg atgaagatgt cgtcgagcgc cacgaagaga ccgaaaggca ccaccgccga
    11281 cacgctgcca acgaactcct cgccgacgcg gtcctgcatg aagtagcact tgaggaaggc
    11341 gaccacgtcg cgggtggcgt cgtcggcgcg gcgctcggtc atcgagcagt gcaggccgat
    11401 ctgctcccag tcgccggggc ggtactgctc gccggcgagc gcggccttga tgccgcggtg
    11461 gatcagcagg tcgggatagc gccggatcgg tgaggtgaag tgggtgtagg actcgtaggc
    11521 gaggccgaag tggccgacgt tgtccgggct gtacatcgcc tgcttgagcg aacgcagcat
    11581 gacggtctgc aacaattggg cgtcggggcg gtccttgacc tgctcgagca gcctggcgaa
    11641 gtcggcggcg cgcggctcgt cgccgccgcc gaggccgagg ccgaactcct tgaggaactc
    11701 gcgcagcttg gcgagcttgt cctcggaggg cgagtcgtgg acgcggtaca acgccgggtg
    11761 ctcgcgcgtg gcgaggaagt cggaggcgca cacgttggcg gcgagcatgc actcctcgat
    11821 caggcggtgg gcatcgttgc gcacctcggg gacgatctgc gcgatcttgc cgttatcgtc
    11881 gaagatcatg cgcgtctcgg tggtctcgaa gtcaatcgcg ccgcgcttcg cgcgcgcctt
    11941 gagcagcacg cggaagagct ggtcgagctg ctcgaggtgg gggagcagcg cggcgagttc
    12001 ggcgcgcacg gccgggtcct tgtcgtacag ggccgccgca accttcgtgt aggtcaggcg
    12061 cgcgtgcgac cagatcaccg cggggtagaa gcggtagttc ctgatctcgc cggtggccgc
    12121 gatgttcatg tcggcgacca tggccaggcg ctcgacctgc gggttgagcg agcacaggcc
    12181 gttggagagc ttctcgggca gcatcgggat gacgcggcgc gggaagtaca ccgagttgcc
    12241 gcgatcgaag gcatccttgt cgagcgcgct ggcggcatcg acgtagtgcg agacgtcggc
    12301 gatggcgacg atgaggcggt agcccttgcc ctggcgctcg caatacaccg cgtcgtcgaa
    12361 gtccttggcg gtctcgccgt cgatggtgac caacggcaac ttggtgaggt cctcgcggcc
    12421 ggcccagtcc ttcttgcgca ccacgtcggg cagcttgcgc gtctgcgcct tggcctcgga
    12481 ggagaactcg aagggcaggt cgtgcttgcg cagcgcgatc tcgatctcca tgccggggtc
    12541 ggcgtagttg cccagcacct cgacgatgcg cccgatcggc tgggcgaact tggtgggctg
    12601 ctcgaccagc tcgacggtga cgatctggcc cggctcgggc ttcttgcgcc cgcccggggc
    12661 gaccaggatg tcctgcgcca ggcggcggtt ctcgggcacg acgatcatca cgccgtgctc
    12721 gttgatgaca cgaccgacga cgcgggagtt ggcgcgctcg agcacctcga ccagcttgcc
    12781 ctcgggacgg ccgcggcggt ccgtgccgga gatgcgcacc atgacgcggt cgccgtgcag
    12841 cacctcgcgc atctccttgg gaccgagaaa gacgtcgggc tcgccgtcgt cgcggcgcag
    12901 gaagccgaag ccgtcggggt ggccctcgac gcgacccttg atgaggtcgg ccttggccgg
    12961 cagcaggtag gcgtcgcggc ggttgcgcac gacctggccg tcgcgctcca tcgcgcgcag
    13021 gcgacggtcg aagaagtcga gctcgtgcgg ggcgatgtcg agcgcgccgg cgagctccgc
    13081 gaggggcatc ggcacgccgc gctcggcgag gatctgcagc acgtattcgc ggctggggag
    13141 cgggttgtcg tactgctgcg tctcacgctc gtagaagggg tcggcgagac ggacggcgct
    13201 ggcctgcacg gctgccgcct tgcgcgcggc ccgactgcgg ccagggccgc gcgccgggga
    13261 gccggtgtgc cgagtgctgc cttgggcggc gggttcgggc gcggcgtgcg cggcaagtgt
    13321 gccgcgcccg gccttgcgcc cgggcgggtt ggcgcgcggc gcaagctcga cctgctgcgg
    13381 cgcggcctgc gttctgcggg agggggcgcc ggagcgctgc tgatcggttt ttttcgtttt
    13441 gcgagtcatt gacaatcttc tttgcgtgct tataatgcgc gtcttcttgc ccagatggcg
    13501 gaattggtag acgcactagt ttcaggtact agcgccgcga ggcgtggagg ttcgagtcct
    13561 cttctgggca ccagtttcac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 gcgaggcgtg gaggttcgag tcctcttctg ggcaccagtt tcacaggaaa gccgctcttc
    13741 ggagcggctt ttttgcgttt gtgccatcgt cgcgcgaagg aaggtgcgcc atgcgggccg
    13801 gacgcgagga tcgcgacttg cgtcgcccct atgaaaaacg gcgcggaatt cgcgaacgct
    13861 gcggatggcg caacattcgc ggacggcgcg ggttggcccc gtgcaggaac gactcgagtc
    13921 gcgatcgagc gtcgtgctgg aatcgcgcgg ggggtgcatt gacccccggc gggcgcggcg
    13981 gccgcggagt gcggccgcac agggtgctgc ggatgctcag gcgccaaagg gatggcggcg
    14041 gaggatggtt tcgtcgcgct cgggaccggt cgaaatgacg tcgatcggga tctcgcagat
    14101 ctcctcgatg cggtgcaggt aggtgcgcgc ctcctgcggc agggcgtccc agctctgcgc
    14161 gccaaacgtg gtgccgctcc agcctttcag ggtctcgtag atcggctcgc agcgcgtgac
    14221 ctcctccgaa cccatcggca gcaggtcgat gcgcctgccg tcgagcatgt agccggtaca
    14281 cagcttgagc tcttcgagac cgtcgagcac gtcgagcttg gtgatgcaca ggccggtcac
    14341 gccgttgatg atgatcgagc gcttgagcgc ggcgagatcg agccagccgc agcggcgctt
    14401 gcggccggtg acggtgccga actcgcggcc cttggtcgac atctgctcgc cgggcacgcc
    14461 cttggtctcg atgtcgagtt cggtcgggaa gggcccgccg ccgacgcggg tgcaataggc
    14521 cttcgtgatg ccgagcacgt aatgcaggcg gccggggccg acgcccgagc ccgccgcggc
    14581 ctgcccggcc acgcagttgc tcgaggtgac gaaggggtag gtgccgtggt cgatgtcgag
    14641 cagggtgccc tgcgcgcctt cgaacagcag cgagccgccc gacttgttga tcgcgtagag
    14701 ctcggccgag acatcggcga ccatcggcag cagctcgtcg gcgtcggcca tggcctggtc
    14761 gaacacggtc tggaaatcga ccggctcggc gccgaggtgc cgggtgagca cgaagttgtg
    14821 gtactcgagg ttggccttca gcttggcggc gaagcgctcg cggtcgaaga ggtcgtacac
    14881 gcgcagcgca cggcgggcga ccttgtcctc gtaggtgggg ccgatgccct tgccggtggt
    14941 gccgatcttg tcgccggcgg acttggcggc ctcgcgcgca cgatcgagcg cggcgtggta
    15001 accgaggatc agcggacagc ccgggctcac gcgcaggcgg tcgcgcacct tgatgccgcc
    15061 ggcttccagc gtgcggatct cggacaacag gtggtgcgca tcgagcacca caccgttgcc
    15121 gatgaagcag gccacgcctt cgcggacgat gcccgagggc accaggttca gcttgtattc
    15181 ggtcttgccg acgaccagcg tgtgaccggc gttgtggcca ccctggaacc gcaccacgcc
    15241 cttggcgtgg tcggtgagcc agtcgacgat cttgcccttg ccttcatcac cccactgggt
    15301 gccgacgacg acgacgttct ttgccatttc cttactctcc ctgtaacggc acgaccgccc
    15361 agcgatcgcc ccgcttcacc agttgccggt cgcagccggc ttcgttccaa gttccttcgt
    15421 gtcccggcag cgccaccatc acgatctcgc cacgtgcgcg cagcgcggag acctcggagg
    15481 ccagccccgc gtcgtcgtcc ggcggcgcca gcaccgcgcc cgccggcgcc gccgggatgg
    15541 gcaggtgcca ggcgagttcg cgcaggtcga ggctgaagcc ggtggccggc cgtgcgcgcc
    15601 cgaacgcctg gccgacgcgg tcgtagcggc cgccgagcgc gagcgcggcg ggcgattcgg
    15661 cgccgtaggc ggcgaacacg acgccgctgt gatagtggta gccgcgcagg tcggcgagat
    15721 cgaagctcac cgggaggtcg cccagcgcgt cggcgagttg gcgcagttcg gcaagcgggc
    15781 cggcgatctc ggcatcctgc ggcaggcgtg cggcggcctc tgcgagcacc tcggggccgc
    15841 cgtagagttc gggcagtgcc agcagcgcgc tgcgcgccgg ctcggagacg tcggcgagca
    15901 tgcggcgcag ctcgggcacg tccttcgcct gcagcaggtc gaagagctct tcctcgcggc
    15961 ccggcaccag ccctgcgcgc gcggcgagcg catggaagag tccgacatgg ccgatgtcga
    16021 tgcgcgaggc aggcacttcg gcgaggcgca gcacctcggc gagcaggcgc aggatctcga
    16081 cgtcggcctc gatgccggca tggccgtaga gctcagcgcc gagctgcagc ggctcgcgcg
    16141 tgccggtgag cgtggacggc agggtgtgca gcacgctgcc gcaataacac aagcgcgaca
    16201 cgccgcggcg gttgagaagg tgggcgtcga tgcgggtgac ctgcggagtc atgtcggcac
    16261 gcacgcccat gctgcggccg gagagctggt cgaccagctt gaaggtgcgc agctcgaggt
    16321 ccttgccggc gccggtggtg agcgagtcga ggtattcgag cagcggcggc atcacgagct
    16381 gatagccgcg cagccggaag gcgtcgagca ggcggcggcg caggctctcc agccggtggg
    16441 cgtcggacgg cagggcgtcc tggatgtggt cgggcagtac ccagcgcatg gcgatgtcag
    16501 agaacgatga aaagcattgc gagccccgcc agcatggaac tcagaccgat gaagcggatc
    16561 tggccgtcgg ccatgctggc gaggcgaagg aaggtctcgc gccatgcggc gggcgcgatg
    16621 aagggcaaca ggccttcgac gatcagcatc agcgcgaagg ccgtgaggag cgagcttgcc
    16681 atgaagacgt cagttgcgcg gcgcgcctcc ggcatccttc atgaaacgga agaaatccga
    16741 gctcggatcg accaccagca cgtcgtcctt gcccgagaaa ctcgcgcggt aggcctcgag
    16801 gctgcggtag aaggagtaga actcgcggtc cttgccgaag gcttcggcgt agatggcggt
    16861 cgccttcgcg tcgcccgcac ccttgacctc ctgcgcactg cggtaagcct cggcgatgat
    16921 cacctcgcgc tgacggtcgg cgtcggcgcg gatgcgttcg gcctccgcgg cacccagcga
    16981 gcgcaactcg ttggcgacgc gcttgcgctc ggcctccatg cgacgataga ccgactccga
    17041 gacctcgttg ggcaggtcga cccgcttcag gcgcacatcg acgatctgca cgccgatggt
    17101 gcgggcgtcg cggtccgcgc gctcgcgcat ctgctccatg atgcggtcgc gctcgccgga
    17161 gacgacgtcg tgcacggtac ggcggccgaa ctcctcgcgc agaccggcgt tgaccgtctg
    17221 cgtcaggcgg gtgcgcgcac gcgcctcgtc gccggccacc gactcgtagt acaggcgcgg
    17281 gtcgacgatg cgccacttga cgaagtggtc gaccagcacg ttcttcttct cggaggtgat
    17341 gaaacgctcg ggttcgggcg tgtccatcgt caggatgcgc ttgtcgaagt agcgcacgtt
    17401 ctggatgaaa ggcagcttgg cgttgaggcc gggctcggag atgacctcct tgacctcgcc
    17461 gagctggaag acgatcgcat actggcgctg gtcgacggtg aacagcgaca tcgaggcgat
    17521 cacgacgagc agcagcagcg tgccgccgat cagggacatc ttgtcacgca tcaacgtact
    17581 ccccgctcac gactgaacat tccatcccgg ttgcgcggat cgaaggacgg caccggagcg
    17641 ttggccgcga ggcccgcggc cgactgcgag tcgggaggcg ccgccgcggc gggcttggcg
    17701 cccgtctgct gcatcagctt gtcgagcggc agcatcagca ggttgccgtt gcccttcgcg
    17761 tcgaccatca ccttggaggt gctggacatg acctgctgca tggtgtcgag atacaggcgc
    17821 tcgcgggtga cctccggcgc gcggttgtat tctgcgaaca cctggctgaa gcggctcgcc
    17881 tcgccctcgg cattggcgac cacgcgctcg cggtaggcgt tggcttcctc gaccaggcgc
    17941 gaagcggtac cgcgggcccg cggcacgacg tcgttggcgt aggcctcgcc ctcgttcttc
    18001 tggcgctcgc ggtcctgccc ggccttgacc gcgtcgtcga acgcagcctg gacctgctcg
    18061 ggcggctggg cgttctgcat ggtgacgcgg ctgacctgga tgccggtctc gtagcggtcg
    18121 aggatgcgct gcatgagctc gtgcgcggtg gtcgcgatct cctcgcggcc ctcgtaaagc
    18181 acgaagtcca tgcggctctt gccgacgatt tcgcgcatcg cggtctccgc ggcctgcgcc
    18241 acggcctcgt cggggaaacg gttgttgaag atgtagttct ccgggctgtt gaggacgtac
    18301 tgaacggcga actggatatt gatgatgttc tcgtcgtcgg tgagcatcag cgattcgcgc
    18361 agcaccttgt tgcgctcgga accgcgatag cccacctcga ccgtgcgcac gcccgtgagg
    18421 tcgacgatct cgtgggtctc gaagggtgcg ggcaggcgcc agcgcaggcc gggctcggtg
    18481 gtggcgacgt actcgccgag gcgcagcacc acggcgcgct ggttggcgtc caccgtgtac
    18541 aggccgctcg ccagccagac caccagcacc agcgccgcaa ggacgccgaa gccgccacgg
    18601 aactgcttga aggagaagcc ggggagctcg gggcggttgc cgccaccatt gccgccgccg
    18661 aagccgccgc cacgcccggg gcgtttgccg ccgaacatgc cggaaaggcg ctggttgaag
    18721 tcgcgccaga cctcctcgag gtcgggcggc ccctggttgc caccgcgatt gcgatcgccg
    18781 cgctggtcgt cgttgcggtt gtcgtcgccg cgattgccgt cttcgcggtc gccgccgcct
    18841 tggccacccc agcgtgggtc gttcagtgac atgagaatgc ctgttatcac gtttcttgtc
    18901 gtcgctcgtc atcccgaccg gcgggccggt cggcgttgtc accaaaggtc tgccgagcta
    18961 cttcggcaag cgcgtcgcga agcagatcga ggccttcccc ggttcgggcg ctcaaaaaaa
    19021 tgcgcctgat ggtaccacag tcccccctct cgaccgccgc agcggcgcgg gtgaggtcga
    19081 tcttgttcca caccaggatc tgcggcacgt cggccgcgcc gatctcggcg agaacctggt
    19141 tcaccgcccc gatctgcgca tcgcggtcct cgctcgccga gtcgacgacg tgcaacagca
    19201 ggtcggcctg cgccgtttcc tcgagagtgg cctggaaggc cgccacgagg gcgtgcggaa
    19261 ggtcgcggat gaagccgacg gtgtccgaca gcacaacgct cgcacctgcc ccctcgccgc
    19321 cgacgtacaa cctgcgcgag gtggtgtcga gggtggcgaa gagctggtcc gccgcgtagg
    19381 cgcccgcctt ggtcagcgcg ttgaacagcg tcgacttgcc cgcattggtg taaccgacga
    19441 gagagacaga cagaacgtcg cggcgttccc gagcgcgccg acgaaccttg cgctgcttct
    19501 cgatctgggc gagccgggat ttgagcatct tgacccggtt gccgagcagg cggcggtccg
    19561 tctcgagctg cttctcgccc ggaccgcgca ggccgatgcc gcccttctgg cgctcgaggt
    19621 gggtccagcc gcgcacgagg cgggtcgcca ggtgctcgag ctgggcgagt tcgacctgca
    19681 gcttgccttc gtggctgcgc gcgcgctgcg cgaagatgtc gaggatcagc gcggtgcggt
    19741 cgatgaccat gcactgcagt tcgcgctcga ggttgcgctg ctgcccgggc gacaaggcgt
    19801 ggttgaagat gacgatgtcg gcgccgtagg cgcgcagcgc ctcgccgatc tcctgcacct
    19861 tgccgctgcc ggcgaagagc ttggagtcgg gcgcggcgcg gcggccttgc acgaccgctt
    19921 cgacgctcgc gccggcgctg aggacgagca gcttcagctc ggacaggcgc tcgtcgatcg
    19981 cgccctgccc gaggtcgagc tgcacgagga ccgcacgctc accggaggcg ggacgttcaa
    20041 acacgacgcg cgcctcccgc agccggcaga cgccggccgc gccgggtgga ccgcggcctc
    20101 agttggcacc ctcttcctgc tggatgacga ccgggcgggc gggcacgacg gtggagatcg
    20161 cgtgcttgta gaccatctgg gtgacggtgt tcttgagcag cacgacgtat tggtcgaagg
    20221 actcgatctg gccctgcagc ttgatgccgt tgaccaggta gatcgacacg ggaatgtgtt
    20281 cgcgacgaag ggtgttcagg aaggggtcct ggaggagttg ccctttgttg ctcattgtgg
    20341 aacctcttta tattgtgttt ataagaatat tgcagacacg ttgtgcactg ccgcatggca
    20401 gtatgcaacg aaatccaggc gtggaaacgg gttctttcgc gaaaaatgct caggtccgcg
    20461 tcgcgtaagg attgtgcgtg gtgcggaact ggatgcgcaa gggcgtgccc ttgaggtcga
    20521 aggcatccat gaaacagcgc tccaggtagc gcacatacga ctgcgggatg tcgtccagcg
    20581 cgttgccgtg gatgacgatc accggagggt tcatgccgcc ctggtgggcg tagcgcatct
    20641 tcgggcgcgc cagcccgtgg cgcggtggcg cctggcgcgc gaccgcctgc tgcagcgcac
    20701 gcgtcagccg cggcgtggcc aggttggcgg tggccgcggc ataggcggca tccaccgact
    20761 tcagcagccc tgcgatgccc tcggccttga gcgcggagat ctggtggaag cgcgcgaacg
    20821 agaggaaggc cagcttgcgc gcgatgtcgg ccttgagacg atcgcggcgg tagtcgtcga
    20881 ccgcatccca cttgttgatc gccaccacca gcgcgcgccc ggcctcgagc gcgaagccgg
    20941 cgatgtgggc gtcctggtcg gagatgtcct gcgcggcatc gagcaccagc acgaccacgt
    21001 tgcactcctg cacggcctgc agggtcttga tgacggagaa cttctccacc gcctcgaaga
    21061 ccttgccgcg gcggcgcagg ccggcggtgt cgatcagggt gtagcgcttg ccaccgcgct
    21121 cgaaggggat cgcgatggcg tcgcgggtgg tacccggcat gtcgaaggcg atgacgcgct
    21181 cttcgccgag cagggtgttc accaaggtgg atttgccgac gttgggacga ccgacgatgg
    21241 cgaccttggg gccctcgtcg tcggagtgct ccttctcgtc gtcggccggg aagggcgcga
    21301 gcacgagctc gaccaactgc ttgacgccgt cgccgtgcgc cgccgagacc ggcagcggat
    21361 cgccgaggcc gagcgcatgg aagtcggcgg cgaccatcgc gcggtcgata ccctcggcct
    21421 tgttgacgac cacatgcacc ggccgcccgg cacggcgcag gcgcgcggcg atgttctcgt
    21481 cgtgcggggt gcgcccggcg cggccgtcga ccaggaacag cagcacgtcg gcctcggcga
    21541 tggcctgctc ggcctggcgc gccatctcgt gcatgatgcc gtccttggcc acggggtcga
    21601 agccggcggt atcgacgacc agatagtcgc ggtcgccgac gcggccgatg ccgtagtggc
    21661 ggtcgcgggt gaggccgggc tggtcggcga cgagcgcgtc gcgggtgcgc gtcaggcggt
    21721 tgaacagggt cgacttgccg acattgggcc ggccgaccag aacgatggtg ggtttcactg
    21781 gctaccgtac ctcagcgtac ttcgtacgcg gtgatgtcgc cgccggcgcc ctgcaccacg
    21841 aaaccgcggc gcagcgggac cggatcggcc acgatcggat cactgccggc gcgctcgcgc
    21901 gcggcgaagg cgccggtctc gcgcgagagc gcatgcacat agccctcgag gtcgccgacg
    21961 atcacgtagt cgccgacgat caggggacgg gagacgtggc ggcgcgcgag cgcgtcctgc
    22021 ttccacacgc tggcgccgct ggcggcgtcg agcgcatgga ccgcatcgcg gtcgtcggtg
    22081 atgagcgcga agcgcgcgtc gcggtccatg ccgacgctgc tggagaactc gcgcgaccag
    22141 atcgcgttgc cgttcgaggt gtcgaagcag gcggcgcggc cctgataggc gaccgcgcag
    22201 acctcgcgac gcccgaccac cggcatgccg gccacgtcgg cgacgcgttc gagttcggtc
    22261 gctccgcgcg gcacggcgac gttgagctcg gtgatcgcgc cgccattggc agcgttgatc
    22321 acggcgagct tgccgccagg gaagccggcg agcacgaccg ggccttcgag gaccacgccg
    22381 gcgaagctgc gcagcgcgag cggcgggttg ttgcgctggt acacccagcg ccggctgccg
    22441 tcgcgcgcgc tgagcgcgat caggcggttg tcggaggcgc gcacgatcac cagctcgagg
    22501 ctgaccgcgg gcggcgccag cacctcggcg ccgatcggcg cgcgccagcg ctcgctgccg
    22561 ttgtcggtgt cgagggcgat caccgtgccg tcggtggcga ccaccaccgc gaggcggcca
    22621 tcggtgccga ccccggagga gagttcgccg ccggtatcga tccgccacac cgtgcggccg
    22681 tcctcgaagc gcgcgacctc gccctcgttg ccggcggcgt atacgctgtc gctccacacc
    22741 gcgggctgga agcggtagtg ttcggcctcg ccgatgttcg cctgccattt ggccacgagc
    22801 tgcgcgccgg gcttgaagtc gaccagcggc gcgggcttgg gtgcggagct ggcgaagggg
    22861 ttgagcgagg agcagcccgc ggcgagcagc gcggcgacga gcagcgggac gagacgcagc
    22921 gagggggttt tcatcgctca ggctccgagg gattcgagtt tgacgcggac gacttcgcgc
    22981 aacgtgaccg catcgtcacc gacggcggcg agcgcgtcga tcgcggcctg gtaggcggcg
    23041 cgcgcctccg cgaccttgcc ttgggcggcg agcacgtcgc cgcgcaggtc gtcgaagcgc
    23101 gccttgtagg cggccgaggg cgcggactgc aggcgggcga gcgcggcgtc gagttcgccc
    23161 gcctgcagca gcaccgccgc cagacgcagg cgtgcgagct cgcgcagcgc ggggtcgctg
    23221 cccttgtcgg ccgcccactc gagctgcgcg cgcgcgttgg cgaggtcgcc ggactcgaac
    23281 tgcatcgccg ccgacagcag cgcgccgagt tgggcgccta cactgccgcc gtgctcgctc
    23341 accaggcgac cggccgcctc gcgggccttc tgcggctcgt tcttctccac cgcctgctgc
    23401 accgcgtagt acagcgcacc ggcctcgccg gcattgcggc tctggtacca gttccaggcc
    23461 tgccagccca ccgaggcgag cgccgccacc accgcgaggg tgacgacgaa gttgccgtac
    23521 tgcgcccacc acgccttgag ttcggaaatc tgttcctgct cttcgagatc gtagaccgcc
    23581 atcatcaaac ctcttcgtct tcttcttcgg ggaacatcag ggcggccatc gcctcgacca
    23641 ggtcgtcgat cgccacgcgc tgctgggcgc ccgcgacgcg caggggcttg aggccgacct
    23701 cccccgccgc ggcctcgtcc tctccgatca cgatcgccac cggcgcctcg ctggcgtcgg
    23761 ccttcttcat ctgcgacttg aagcttccgc caccgcaatg catcagcacc gcgaagccgt
    23821 gctcgcgcaa ggtctcggcg gcgcggaaac cgaggcgctg cgcggcctcg cccacgctca
    23881 ccacatacac gtcgggcacc ggacgctcgg cctcgccacc gcaggcctgc cacagcagca
    23941 gcaggcgctc gatgccgatc gcgaagcccg cggccggctg cggcttgccg ccgagctgct
    24001 cgaacaggcc gtcgtagcgc ccgccggcgc agatcgtgcc ctgcgcgccc aggcgcgtgg
    24061 tgacccactc gaacaccgtg cggttgtagt agtccaggcc gcgcaccagg cggtggttga
    24121 tgcgatacgg gatgccggcg tccttgagga agacctgcac cgcctcgaag tgcgccttcg
    24181 attcgtcgcc gagatagtcg gcgagcctgg gcgcggcttc gacgatcgcc tgcagttcgg
    24241 gattcttggt gtcgaggatg cgcagcgggt tggtgtacag gcggcgcttg ccgtcctcgt
    24301 cgagcttgtc ctgatgctgc tcgaggtggg cgatcagcgc ggcgcggtgc tgcgcgcgct
    24361 cctcgggcga gccgagcgag ttgatctcga gcgcgacatc ctccaggccg aggtcctccc
    24421 acagccgcgc gcacatcagg atcagctcgg cgtcggtatc ggctccggcg aagccgagtg
    24481 cctccacgcc gatctggtgg aactggcggt agcggccctt ctgcggacgc tcgtggcgga
    24541 acatcgggcc gtagtagtac agccgctgcg ggccgcccgc ggggatcagg ttgtgctgga
    24601 tcgcggcgcg cacgcaggag gccgtgcctt cggggcgcag cgtgaggtgc tcgccgttga
    24661 gcgcgtcctc gaaggagtac atctccttct cgacgatgtc ggtgacctcg ccgatggcgc
    24721 gcttgaacag cggcgtcggc tcgaccagcg gcatgcggat cgggcggtaa ccgtagctct
    24781 gcagccagtc gcgcacgatg tcttcgaagt attcccaggt ttcggcgtcg gccggcagga
    24841 tgtcgttcat cccgcgcacg gcctgcaagg tctggctcat gtggttcttt tcggttgtgc
    24901 cgcgctcgcg cgcggcggtc gttgttcgtt gcggcgctca ggcgcccttc ttcacgtagc
    24961 gggtggcgac gtaatcgtcg acgagcgcct tgaactcggc agcgatgttg tcgccgcgca
    25021 gggtgacgac cttctcgccg tcgacaaaca ccggcgccgc cggggtttcg ccggtgcccg
    25081 gcagcgagat gccgatgttg gcgtgcttgc tctcgcccgg gccgttgacc acgcagccca
    25141 tcactgccag cgtcatgttc tcgacgccgt cgtactgttc gcgccacacc ggcatctgcg
    25201 cgcgcacgta gtcctggata ccggaggcga gctcctggaa gaaggtgctg gtggtgcggc
    25261 cgcagcccgg gcaggcagtc accatggggg tgaaggcgcg caggcccatg gtctgcagga
    25321 tctcctgcgc gaccacgacc tcctgggtgc ggctgccgcc cggctcgggg gtgagcgaga
    25381 tgcggatggt gtcgccgatg ccttcctgca gcagcacggc gagcgcggcg gtggagccga
    25441 cgatgccctt gctgcccatg ccggcctcgg tgaggcccag atgcagcgcg tagtcgctgc
    25501 gccgggcgag atcgcggtac accgcgatca ggtcctgcac gctggaaacc ttggccgaca
    25561 ggatgatgcg ctcgcggccg aggccgtatt cctcggcctt ggccgcggat tcgagcgccg
    25621 agacgacgag cgcctcgcgc atcaccgcgc cggcgtcgcg cggctcggca cgcttggcat
    25681 tggcgtccat gatgcgggca aggaccgact ggtcgaggct gccccagttc acgccgatgc
    25741 gcaccggctt gtcgtagcgg caggcaagct cgacgatcgc ggcgaactgc gggtcgcgct
    25801 tgcggccggc gccgacgttg cccgggttga tgcgcagctt ggcgagcgcc tcggcgcacg
    25861 ccgggaagtc ggtgagcagc ttgtggccgt tgtagtggaa gtcgccgacc agcggcacgt
    25921 ccatgttgag cgcgagcagg cggtcgcgga tcttcggcac cgccgccgcc gcggcctcgt
    25981 tgttgaccgt gatgcgcacg atctcggagc cggcgcgggc gagctcggcg acctgcatcg
    26041 cggtggcgag cacgtcggcc gtgtcggtat tggtcatcga ctgcacgacg accggggctt
    26101 cgccgccgat cctcaccttg ccgacgcgca cctgatgggt gcgatgacgg gcgagcgggc
    26161 gggcttcgat cggctggagt tcgtgtcggg gattcatggc gttcggatca gggaagcgtc
    26221 aggcgggcga cggagccgcg ggtgtgggct gcgagatcga ccgccttgcc gtcctgctcg
    26281 agcacgacgt tggcggagtt gcccaccacc aggcggaagg gcggcgtgcc ctggaccacc
    26341 cggctgctgc cggcacggtt ggtgccggag tgcaggatgg tgccgctggc gtcgcgcacc
    26401 tcgatccagg agtcgccgcc gaagcggaag ctgaggcggt tgccgccggc accttcctcg
    26461 gcgacggggg tggccgcctg ggccggcgac tgcaccacgg gagacacgat ggccgaggct
    26521 gaggccgggg gcattgcgct cgccggcgcg gcgagcgggg tcgccgggac gcctgcaccc
    26581 accgcagcgg gaatcgccac cgggctggcg tgcggcgccg cgggcgcgct cgcctccagc
    26641 ggagcgggcg tggccaccgg ctcgctcgtg gccggcgcgg cgccgccacc ctcggtgggc
    26701 gcgggcatga aggcgggcgc aggctcggtc tgcagttcga gcccgggcag gggctcggtg
    26761 cggaaccagt cgaagtacca gcccacgccg agcaagacca gcaacaaggc cacgacgacc
    26821 ccagccggga agctgccgcg acgcccgccg ccaccggcgg gcaggtcgcc atcggcattg
    26881 cggatcggcg acaggtcggg ctgcccggcg ccggccaggc gctcgatgcc atcgagcagc
    26941 ggcgtcgcgt cgagtccgag gtaacgcgcg tagttgcgcg cgaaaccacg cacgaaggcc
    27001 atgcccggga gcgcggcgaa gtcgtcgttt tccagcgcct cgatctggcg cgggctcagc
    27061 ttgatggcga aagcggcctc gtgcaccgac tcgccacgcg cctcgcgcgc ccggcgcagg
    27121 cgggcgccgg gcgaggcggc ggactcggcg ccaagtgcaa ggtggtcgga ctgcatgctg
    27181 ctcactcgaa cttcccttga ttcatcgatt tgaactcctc cgattcggcg aagcggctgc
    27241 gcaactgggc agcatagctc gcctcggcgt cggcgttgcc gaggcggcgc tcggtgcgca
    27301 ggcccaacca ggccgaagcc gcgctcggtc cgtgcgcctg gtgcagccgg atcagctcgc
    27361 tgcgcgcctg ctcgtagcga ccgccgcgat aggcgatgtc cgcgagcagg tagagtgcct
    27421 gcgcgttctc cgggtcggcc tgcaccgcgc gctggaattg cgcctcggct gcagcctcct
    27481 cgccgaggcg cagatggcac aggcccgcgt tggtgagcgg gcgcgtctgg tggcggtaat
    27541 acgggttgcg cgccgcgagc gcgaggtact cgaggcctgc gcgctcctgg ccctgagagc
    27601 acaggaacca gccgtagctg ttgttgaagt cggggtcacc cggtgcctcg cgcagcgcac
    27661 gctcgaagtt ctcgcgcgcg gcggcgtgct cctcgacgga catgtaggcc agcccgagca
    27721 ggtggtaggc cggggcgtag gacgggctgt cggcgagcgc gatcttcgcc tcgtcgagcg
    27781 cgacgtcgta gcgccccacc tcgaagtagg ccatgccgag ctccacatgc acgcgtgcgc
    27841 gcgcatcggc cggcgtcgcc gggttggtgt cggacatcgg ccggctgatc gaggacgacc
    27901 cggcgggtac gccgggcgca cccgcgcagc cgccgagcac gagcgccacc gccagcacga
    27961 ccggtgccag tgtcgatttc atcgcatgtt ctccctcgcc tggtgcaggc gcacggtacg
    28021 ccgcgtcctg tcctggacct ggccggcgag ctggccgcac gcggcgtcga catcgtcgcc
    28081 acgcgtcttg cgcgtggtgg tcacgatacc ggcgtcgatc aggataccgg caaaacgccg
    28141 gatacgctcc gcgggcgagc gctggaagcc ggagtccggg aaggggttga acgggatcag
    28201 gttgaacttg cacggcgtgt cgcgcaccag cgcgacgagt tcgcgagcgt gtgcgtcgct
    28261 gtcgttgacg ccttcgagca tcacgtactc gaaggtgacg aagtcgcgcg gcgcgcgctc
    28321 gaggtagcgc tggcaggcgg ccatgagctc gcgcagcggg tacttctgat tgatcggcac
    28381 gaggcggtcg cgcagcgcgt cgttggacgc atgcagcgac accgccagcg ccaccgggca
    28441 ttcgtcgcgc aggcggtcca tcgccggcac gatgcccgag gtcgacaccg tgacccggcg
    28501 gcgcgacagg ccgtaggcgt ggtcgtccag catgaggcgc agcgcggtga cgacgttgtc
    28561 gaagttggcg agcggctcgc ccatgcccat catcaccacg ttgctgatga tgcggccgtt
    28621 gtccttctcg ccggcctcga ggtccgtggc gtgctcctcg gcgtcggccc gcgccgcgcc
    28681 gagcagcttg ttggccagcc agagctggcc gatgatttcc gcggccgaca ggttgcggtt
    28741 gaagccctgc ttgccggtgg agcagaacgc gcagtcgagc gcgcagcccg cctgcgagga
    28801 cacgcacagc gtgccgcggc tggtctcggg aatgaacacg gtctcgaccg cgttggcgtt
    28861 gcccacgtcg agcagccact tgcgcgtgcc gtcggccgag atcgagtcgc gcaccggcac
    28921 cggcgggcgg atcacggcga ggtccttcag cttcgcgcgc agggacttgg cgacgtcggt
    28981 catggcgtcg aagtcgtcgc agccctcgtg atgcatccag cgcatcacct ggcgggcgcg
    29041 gaacggcttc tcgcccagcc cggcgaacca ggcgacgagg ccgtctacgt cgaaatcgag
    29101 cagattgacc ggattgggag tgctcatgcc tgaaacttca cctctcggga gcggaggccc
    29161 gcgatgacgg gccgaccgta atgcaaacgg cccgctcacg ccgcgcgcgg tgcgggaggc
    29221 accgcgaagc gtgcgcgacg ggccgctggt acggaccgct cagcgcgagt aaacgttcat
    29281 gcccgggaag aagaaagcca cttccacggc agcggtctcg ggggcgtcgg agccgtgcac
    29341 ggcgttggcg tcgatcgact cggcgaagtc ggcgcggatg gtgcccttgt cggccttctt
    29401 cgggtcggtg gcgcccatca gctcgcggtt cttggcgatg gcgttctcgc cttccaggca
    29461 ctggatcatc accgggccgg aggtcatgaa ggagaccaga tccttgaaga aggggcgctc
    29521 cttgtgcacg gcgtagaact ggccggcttc ct
//
LOCUS       DAOQBS010000026        29219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_954_length_29219_cov_15.597963, whole genome shotgun sequence.
ACCESSION   DAOQBS010000026 DAOQBS010000000
VERSION     DAOQBS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 29219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29219
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_954_length_29219_cov_15.597963"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PKN25_04990"
     CDS             <1..1209
                     /locus_tag="PKN25_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNV90288.1"
                     /translation="PFGMPVEVQIRTREMHHIAETGVASHWLYKEDEKTLTELQQKTH
                     SWLQSLLELQSASGEATEFLEHVKIDLFPGEVYVFSPKGKIFSMPKGSTPVDFAYAVH
                     TDVGNRCVACRINGDLMPLRSELRNGDQVEIITAAHANPNPAWLSYARTGKARAQIRH
                     FMKNAQQDESVTLGERLLNQALRPHGLTLGQISSFAWDRFLRDRGVRHKKEVFSDLGL
                     GKRLPVIVARRLAEAQDLESGRPDVVKPKPAGAISIRGSEGVAVQLARCCHPIPGDPI
                     IGTLRKGQGLEVHTHDCPNVAKLRGDRGRWVDVEWEHMVDDRLFDVGIRVLSHNACGV
                     LARVASAIAEEDSNIQSVSMDGGQGEYTALNFTVQVHDRMHLARVMRGVRRVQEVVRI
                     GRVKADGRVG"
     gene            1297..1485
                     /locus_tag="PKN25_04995"
     CDS             1297..1485
                     /locus_tag="PKN25_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HNV90289.1"
                     /translation="MAMYESDHTKFMREWMEKHPEQRDEQKEGRALWWDKPQSVEEQK
                     RVAAARVPVKPYYYDINN"
     gene            1598..3046
                     /locus_tag="PKN25_05000"
     CDS             1598..3046
                     /locus_tag="PKN25_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNV90290.1"
                     /translation="MSLPSRLAFIDVESTGAHPVRDRITEIAILRVEDGEVVARWESL
                     VAPGQPIPPLIEEVTGISDAMVAEAPAFEVLADTVAALLEGCVFVAHSARFDYGFIRN
                     AFTRIGREFEAPVLCTVKLSRALYPEHHRHGLDALIERHGLVCAARHRAMGDVEVLWQ
                     FVRGLEAGFAPEVLARAAARAMKAPTQPPGLPEGMLEALPDAPGLYLFHAQPEPPPAG
                     ARDPALFVGRAQSLRARVREHFGAGARKGRDAELAARVKRVEWIETAGELDTSLRENA
                     LLRALAPPYNRPQEPAEAAFALRLLPRRRRAPIYETVAIAGTDPADWHDLHGVFRNRR
                     EADNLLRELALLYRLCPRRLGLEGGSGPCTAHAARRCAGVCARRETPEEHDARLAGAL
                     AGVGVKPWPWAGPVAVEEVRAHGPHRLQVFDRWCHLGSAQDEAGALALAAAPRAFDAD
                     VWRILARWLAVPAHLAQVRVLAAQAGEGERGG"
     gene            complement(3100..3801)
                     /locus_tag="PKN25_05005"
     CDS             complement(3100..3801)
                     /locus_tag="PKN25_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNV90291.1"
                     /translation="MSCRPPSLAPDASDLLARFAPFDMLDPCARERVLAAARPIHAVR
                     GELLVQQEDCPRGIYLVLDGRVKCFVLSPSGGEKIVRLAEPGSFFGEEAALINRPHVA
                     NAQAIGTSELLLVPTAALRTAMVSSTAFSVSMSNRLAEASYDLMSTMQVQFQLSGTQR
                     VAHYLTRLAPEDAERCEIRLDIDKQTIAAQLNLTPETLSRILSQLTRDGMIQPRGRRG
                     MVLSKLSQLRDCAAH"
     gene            4058..5971
                     /locus_tag="PKN25_05010"
     CDS             4058..5971
                     /locus_tag="PKN25_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNV90292.1"
                     /translation="MYLAVPRLLVRRSIMLLVSVALVAMTLIGLAGMSASFVVVDRSR
                     DSVQAIAVASTLRGHTQRIANLIAIDALKGRIGVSERTRAAMADIERELQQAALRRFV
                     DEAPGELFAATYRGVQAGWERSVRPRIEALAGPVPPDAREVETLLAGVDDFVGQVDSL
                     VAVLGQENERRIDELRRILALAAAVTLGVVLVVIVLLQRALLRPLGGLLDAARRIAGG
                     DFGVRVRYTGEDELGQVGSAFNLMADELARHYHLLELRVTEKTAELQRSNRSLELLYH
                     AIARLYQAPSAPDAYEATLRDIDRVAGLEGSFVCIEPRPGAPAAVIASSMGPCPDRAE
                     RGEDACAACRAGSEGQALALLSPTLLRFPLRDREHHHGMLRLSLSDGARLEDWQRQLV
                     EALSRHIGMALGAARRTEQERLLALQEERSVIARELHDSLAQALSYMKIQVSLLQRAL
                     ADPARTAEAEPILADLREGISAAYRQLRELLVSFRLGLSADLATLMEDAAREYGTRGG
                     LEVELAVELGACQLSPNQEVHVLQIVREALSNMVRHASARHAWVALRGGADGEVLLEV
                     RDDGCGIGAPPADARNHHGLAIMRERARSMGGEIDIGPALPSGTRVCVRFRSANASMA
                     AMAQGDGKKETET"
     gene            5968..6612
                     /gene="narL"
                     /locus_tag="PKN25_05015"
     CDS             5968..6612
                     /gene="narL"
                     /locus_tag="PKN25_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HNV90293.1"
                     /translation="MNESCRVLIVDDHPLFRRGLVQLLRTIPAFVLVGEAASGAEGLV
                     LARELRPDLVLLDLNMRDGDGLDMLRGLRAARSEARVVMVTVSDSGEDVVAALRGGAE
                     GYLLKDMEPEAMLEALQAVAAGRVVIPPQLNHLLAAALRGESRPQSAGAAGLTEQELR
                     ILEKIAAGLPNKQIGRELDIAEGTVKVHVKHILRKLELRSRVEAAVWAVEHLRA"
     gene            6738..7274
                     /gene="napF"
                     /locus_tag="PKN25_05020"
     CDS             6738..7274
                     /gene="napF"
                     /locus_tag="PKN25_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HNV90294.1"
                     /translation="MASSASPRAASSRRNFLFGRTRAVEPALRPPWALEEDDFVARCT
                     RCERCIEACPTTILVRMDGGFPGVDFSRGECSFCGDCVAACEPRALWREDASAAPWDR
                     VAVIGAACLAARGVECRVCGEACPEGAIRFRPRLGGVAHPELDAAACTACGACIGPCP
                     TRAIDTRVRNPLPTESAT"
     gene            7271..7525
                     /locus_tag="PKN25_05025"
     CDS             7271..7525
                     /locus_tag="PKN25_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HNV90295.1"
                     /translation="MNIASLVVRAFPADFPEIVEGLKKVPGVELHGQSAEKGNIVITI
                     EDGAGWSVTDSILAVNLLPKVQGLTIAYEYTDEGLELTEV"
     gene            7527..10073
                     /gene="napA"
                     /locus_tag="PKN25_05030"
     CDS             7527..10073
                     /gene="napA"
                     /locus_tag="PKN25_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HNV90296.1"
                     /translation="MSMNRREFIKNNAIAAAAATAGIGIPVVAEAQQPGAGGTKVRWD
                     KAACRFCGTGCSVLVGVQDGRVVATQGDPDSPVNRGLNCIKGYFLSKIMYGEDRLTKP
                     LLRMKNGQFDKNGEFTPISWEQAFDIMEQKWKKAIKEHGPDSVAMFGSGQWTVWEGYA
                     ASKLMKAGFRTNNLDPNARHCMASAVAGFMRTFGIDEPMGCYDDIENADTFVLWGSNM
                     AEMHPILWSRITDRRLSKQGCQVHVLSTFEHRSYELADNPMIFVPQTDLAILNYICNY
                     IIQNGKVNKAFVERNVKFKIGETDIGFGLRPNHVLEAKATSNGYPGAEGKPKGDTGAA
                     KDISFDEFARFVSEYTAEKVSKLSGVPEDRLVKMAEQYADPNRKVVSFWTMGFNQHTR
                     GTWVNNMIYNVHLLVGKISEPGNSPFSLTGQPSACGTAREVGTFAHRLPADMVVTNPM
                     HRALTEELWKLPEGTIPPKIGLHAVAQSRALKDGKLKCYWTTTTNNMQAGPNVNDEIY
                     PGWRNPEAFVVVSDVYPTVSALSADLILPCAMWAEKEGAFGNAERRTQFWRQQVSAPG
                     EAKSDLWQYIQFAKRFKVEEVWPEELVAKKPEYRGKTLYDVLYANGQVNKFPLADVEK
                     ANGHAIPGYMNDESKELGFYLQKGLFEEYAAFGRGHAHDLADFDVYHKARGLRWPVVD
                     NKETLWRFREGYDPYVKAGEGVKFYGHKDNKAVVFALPYQDPPEMPDAEFDMWLCTGR
                     VLEHWHTGSMTRRVPELHRSVPEAQIFMHPEDAQKRGLQRGMAVKVVSRRGEIVARLE
                     TRGRNKPPVGLIFVPFFDEGRLVNKLTLDATCPISKETDYKKCAVKVLKV"
     gene            10137..11036
                     /gene="napG"
                     /locus_tag="PKN25_05035"
     CDS             10137..11036
                     /gene="napG"
                     /locus_tag="PKN25_05035"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HNV90297.1"
                     /translation="MLMNDKVQSTGRVEPVAPASRRRFLKDAAGVAGGAGLLALGAGM
                     YAKQASALPATAIRPPGALPEDDFLAACVRCGLCVRDCPYKTLKLAEFGDGVATGTPY
                     FEARDIPCEMCEDIPCVVACPTGALDRALVDITKAKMGLAVLIDQENCLNFLGLRCDV
                     CYRVCPVIDQAITLERMHNPRSDRHAMLLPTVHSEHCTGCGKCEKACVLPGEAAIKVL
                     PIKLAQGSKAEHYLRGWEEKEAAGKSLIGDQVELPVRGLEGKAYGDTRVEPGQGPVQV
                     PAQVPDYVPAKPAGGGLDSGWKP"
     gene            11033..11968
                     /gene="napH"
                     /locus_tag="PKN25_05040"
     CDS             11033..11968
                     /gene="napH"
                     /locus_tag="PKN25_05040"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HNV90298.1"
                     /translation="MSARLGAGGVPERKVPPTSVRGAAMRGVVARPGREAVEAKGWLR
                     AHKWLLLRRASQLGILALFLVGPWLGWWIVKGNLSSSLTLGVLPLTDPFLVVQQLAAG
                     HWPYREALLGAGIVLAFYLLFGGRLFCSWVCPVNLVTDAAAWLRRRLGLKGGKVPSGS
                     TRYWLLGFVLVIAAATGSLAWEWVNPVSMFHRGLIFGFGLAWGIVGGIFFYDLLIAPR
                     GWCGHLCPQGAFYALLGRAAVVKVSAAKRSACNDCMDCFAVCPEPQVIRPALKGVGQD
                     HPLILDADCTTCGRCVDVCGKDVFRITTRFNRSES"
     assembly_gap    11970..12069
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12070..12510
                     /locus_tag="PKN25_05045"
     CDS             <12070..12510
                     /locus_tag="PKN25_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HNV90299.1"
                     /translation="TRNLVLALVAAVGFGAMVPPAAMAQAVKSIRGADVAEQELQVGN
                     YKPIPDQAPIERDYVQQPPLIPHKVEGYEVTINFNKCMDCHAWSRYKESGATKVSLTH
                     FKDRDGGELSNISPRRYFCMQCHVPQTDAKPLVGNKFQRAEGLR"
     gene            12534..13154
                     /locus_tag="PKN25_05050"
     CDS             12534..13154
                     /locus_tag="PKN25_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNV90300.1"
                     /translation="MTDNNKSLMQRIRGAGWGAIVLLVAAGVVFWGGFNWALEMTNTE
                     KFCISCHEMEQNVFKEYRNTIHYQNRTGVRATCPDCHVPKEWGPKMIRKIKASRELYG
                     KVMGTIDTPEKFAAERLRLAQNEWNRMKANNSQECRNCHNYEYFDYSVQGRRSNQMHQ
                     AGFAEGKTCIDCHKGIAHSLPPVDQHIGAPREGVAPEVMHPPAKKD"
     gene            13273..13899
                     /gene="ccmA"
                     /locus_tag="PKN25_05055"
     CDS             13273..13899
                     /gene="ccmA"
                     /locus_tag="PKN25_05055"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HNV90301.1"
                     /translation="MLEVESLACLKGDRLLFRDLAFRLQAGGLLRVAGPNGVGKTSLL
                     RLVTGLALPEAGEIRWRGESIRRAREAFHGDLLYLGHAAALNDLLSPLENLRFACAAA
                     GDAVDAEDCVQALQRIGLADQLDLPARVLSQGQRRRVGLARLFLSNARPLWVLDEPFT
                     ALDVRAVADLAATLSQHCEAGGMVMLTTHQDAPFARPPEVLDVGVFAC"
     gene            13893..14561
                     /gene="ccmB"
                     /locus_tag="PKN25_05060"
     CDS             13893..14561
                     /gene="ccmB"
                     /locus_tag="PKN25_05060"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNV90302.1"
                     /translation="MLSTFLAVLRRDLLLAWRGRADVLVTLAFFIIVVCLFPFGVGAE
                     PNQLRAIAPGVLWVAALLACLLSLHRLFAQDYSDGTLEQLLLSSEPAALWVMAKVLAF
                     WLSTGLPVVAVAPAMGLLLDLEQGGLPVLILSLLLGTPILALLGAVGAALTLGLRGGG
                     MLLALLVLPLFVPVLIFGAGAVEAELSGSGAAAHLLLLGGGLAGALALAPVACAAALR
                     ISTD"
     gene            14619..15404
                     /gene="ccmC"
                     /locus_tag="PKN25_05065"
     CDS             14619..15404
                     /gene="ccmC"
                     /locus_tag="PKN25_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HNV90303.1"
                     /translation="MTNPERGRSLLRFAAPQNFYPLAGRLAPWFAASAVVLTAVGLWL
                     GFFVAPTDATQGQVYRVIFIHVPAAWMSMFIYLVMAFWSAVGLVMNTRLSFVMSQALA
                     PTGAMFCFVALWTGALWGKPTWGAYWVWDARLTSQLLLLFLYFGFIALTRAIEDPRRA
                     DRAGAIIALVGAVNVPIIYFSVQWWNTLHQGASVSLTKAPSMATTMLTGMLVMALASW
                     AYTLAVVLWRVRPMILERERHTEWVGAELERAARLSQTAGGRA"
     gene            15404..15634
                     /gene="ccmD"
                     /locus_tag="PKN25_05070"
     CDS             15404..15634
                     /gene="ccmD"
                     /locus_tag="PKN25_05070"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HNV90304.1"
                     /translation="MQQWESWSAFWDMGGAAFYVWGSYGLAFALIALELVLVFQRRKD
                     TTRRLLRWRRAVGKDAGKRNGEGTMPAMESET"
     gene            15631..16074
                     /gene="ccmE"
                     /locus_tag="PKN25_05075"
     CDS             15631..16074
                     /gene="ccmE"
                     /locus_tag="PKN25_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNV90305.1"
                     /translation="MKARSKRLMLVGGGVALLFAAVALVLSAFQQNLVFFHTPTEVSE
                     GKAPTGKTFRIGGMVEDGSIQREADGVTVRFAITDTARVIPVTYKGTLPDLFKEGKGA
                     VVQGKLEGGVFRATEVLAKHDENYMPPEAAHAVEQAQKAAQTLSQ"
     gene            16076..18052
                     /locus_tag="PKN25_05080"
     CDS             16076..18052
                     /locus_tag="PKN25_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HNV90306.1"
                     /translation="MIPELGHFALILALVLSLVQAVVPLVGAQRNSSALMAVGRPAAQ
                     GQFFFILFSYFCLTWAFIQSDFSVQLAATNSHSATPLMYKITGVWGNHEGSLLLWAMS
                     LSLWTVAVTVFSRHLPDAFLARVLGVLGLVSAGFISFTLVTSNPFDRLLPAVAEGRDL
                     NPLLQDPGMIIHPPLLYMGYVGFSVAFAFAIAALLSGRMDAAWARWSRPWTTIAWVFL
                     TAGIAVGSGWAYYELGWGGWWFWDPVENASFMPWLLGTALMHSLAVTEKRGAFRSWTV
                     LLAIGAFSLSLLGTFLVRSGVITSVHAFATDPRRGLYILALLVIVIGFSLLLYAMRAN
                     KLMGGGSFGLVSRETALLANNVLLTVASASVLLGTLYPLFLDALGLGKISVGPPYFEA
                     VFVPLMTPVVVLMVFGPFLRWKDDELLAEVRRVAPAFVASVAIGVGSAWAVDHLTWRT
                     ALGLSLAAWVVLASLQLLVRRLGERAGASAGSRLRSITASWWGMWLAHFGIGIFIIGV
                     TMVGSLDRHLDVKMKEGQHAELAGYTFVFRGAVDADGPNYDAARGRIELLRDGRSLDM
                     LTPEKRVYLAQGMPMTEASLDIGVFRDVYVSLGEQLEDGAWIVSLYYKPFISWIWAGC
                     VLMGLGGVFAAADRRYRRLAARDAAAAVGSRVAA"
     gene            18071..18664
                     /locus_tag="PKN25_05085"
     CDS             18071..18664
                     /locus_tag="PKN25_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HNV90307.1"
                     /translation="MKAKFLVPLLLFFGLAGFLAFGLTLNPREVPSPLIDKPAPNFRL
                     ARLDQPEQTFALEEMRGQVWLLNVWASWCVACRQEHPVLVRMAAQKLVPVVGLNYKEV
                     RGDGAISTRGMALEAETTMAIERARRWLADHGNPYLLSVLDIDGRVGIDFGVYGVPET
                     FLIDREGRIRYKHIGPITPESLQTQIMPKVEELRRAG"
     gene            18780..19259
                     /locus_tag="PKN25_05090"
     CDS             18780..19259
                     /locus_tag="PKN25_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNV90308.1"
                     /translation="MTPAGIATASDPGVAPAVAADPKLEADMMELSHKLRCLVCQNQS
                     IAESNAPLAVDLRNQVREQLAAGKDKDDVIDYLVERYGDFVLYEPPFKAVTGLLWVGP
                     FALLLGGAGWLAWRLRRRRSEIAAEGALSAADRERARALLAGGGDAASNPASPESRK"
     gene            19256..20479
                     /gene="ccmI"
                     /locus_tag="PKN25_05095"
     CDS             19256..20479
                     /gene="ccmI"
                     /locus_tag="PKN25_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HNV90309.1"
                     /translation="MTAFLVFAGLLVAGALLLILPPLLGYGRRSQAQMRQSAMVLTVL
                     REQLADLDAELAEGRIDAATHAKSREELERRALEEAEVAAETADSADRRPSKAWALAT
                     GLAIPALAVGTYLMIGEPAALDPKNVEGQQGFTQEQVTEMVGSLEKHLAANPDNAEGW
                     AMLARTYLVLKDFPKAAAAYERLDKLVPNDPDVLSDWADVVAAQTGKVGSDAEALVLR
                     ALEAEPKHPKALALAGTAAYQRDDFAAAAGYWERILALIPPGDDVARGVRASINEARA
                     KAGMAPLATNEGNAAPPPASPLKVAGRLQVEGALAGQVAAEDAVFVFVRGPAGGPPLA
                     ALRFKGSELPVNFDFDGARLMMGDAAVPERVVIAARVSKGGDATARPGDLEGVSAPVA
                     PDAEGVTVTIDRVRE"
     gene            complement(20593..21678)
                     /gene="alr"
                     /locus_tag="PKN25_05100"
     CDS             complement(20593..21678)
                     /gene="alr"
                     /locus_tag="PKN25_05100"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNV90310.1"
                     /translation="MRPARALIDLSALRHNYLLARERHGGRALAVVKANAYGHGALRC
                     AQALRDEADGFAVAFLGEALELRAGGIDAPILLLEGVFAADELAEVARQRLWMVVHHE
                     EQLCMLERTPLAAPVDVWLKVNSGMNRAGFVGDAVARAWRRLQASGKVGRIVLMTHFA
                     RADEPELGTTLEQLQRFEAATAGLPGARSVANSAAILAWPQARRDWARPGILLYGADP
                     MPAEGNGLRAVMTLESAVMAVREIAPGEALGYGGRFVAERPTRVGLVAMGYADGYPRV
                     VPDGTPVAVEGRASRIIGRVSMDMLTIDLTALPEAGLGSQVELWGKQVPINHIAQAAG
                     TISYELLCNVKRVRLEYADARGVGGKA"
     assembly_gap    21803..21902
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22129..23454)
                     /locus_tag="PKN25_05105"
     CDS             complement(22129..23454)
                     /locus_tag="PKN25_05105"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HNV90311.1"
                     /translation="MNVIVLGSGVIGTTTAWYLAQRGARVTVIDRQPGAALETSYANA
                     GQVSPGYSTPWAAPGIPLKALKWLFQRHAPLAIRADGSLFQLRWMAQMLANCSSARYA
                     VNKERMMRVSEYARDCLRTLRADTGIAYEERSRGTLQLFRTQAQLDAAARDIAVLEEC
                     GVPYELLDGDALAGAEPALARRPGKLAGGLRLPNDETGDCQLFTTRLAAMAEAAGVTF
                     RFGTEVRELVRRGGHIEGVRIAGADGVEHTLVADRYVLAFGSYSRALAASLGLDLPVY
                     PVKGYSLTVPLVDAARAPVSTVLDETYKVAVTRFDERIRVGGMAELGGFDLRLNPRRR
                     ETLELVVNDLFPGGGDVKAAEFWTGLRPMTPDSTPIIGATPFPELFLNTGHGTLGWTM
                     SCGAGALVADLVVGRQPAIRHDDLGLARYGAEHRSIPVAGARQRPLGTV"
     gene            23588..24055
                     /locus_tag="PKN25_05110"
     CDS             23588..24055
                     /locus_tag="PKN25_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNV90312.1"
                     /translation="MRDLDRTDLKILDLLQKNGRLTMTELAHQVGLSATPCTERVRRL
                     EREGVITGYHARVDPRALGRPLLVFVELKLAAKSNDAFERVKKELAFVPEVMECHLVS
                     GDFDYLIKARISEMSDYRRLLGNILLKLPSATESRSYVVMEEVKESLYIPPMS"
     gene            24096..24914
                     /locus_tag="PKN25_05115"
     CDS             24096..24914
                     /locus_tag="PKN25_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV90313.1"
                     /translation="MSSERGTSIRALERGIEVLRLLGPQEGSSLRALHAASALPKPTL
                     LRILHTLERGGLAWRSIDDGLWRRSLAWTAAPSGHAIDPRLVEAAAPHLVALQRTVLW
                     PSDLLVYRDFAMEVAESSRRLSGLALNPRYRIGYRVDILLSAPGRAWLAFCAAAEREA
                     VLAHWRAASPSNPRTAAVLEGEIDALLEETRQRGYAVRDALFGGSFEDMAGFDDGFDA
                     IAVPILADGGVRGAINLVWPRRYDLRAKVVEAHLADLQACAAAIAAALASPRQP"
     gene            complement(24941..27004)
                     /locus_tag="PKN25_05120"
     CDS             complement(24941..27004)
                     /locus_tag="PKN25_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNV90314.1"
                     /translation="MRLPALLLACVLMVPAAFAAAPQAGARPDHVADRDCLACHPDQA
                     DAWAESKHRHAMQPATAQTVLGDFGEAGSGEVRFGEGAAAARFYRRGGDFMVAAQGPD
                     GRTQALAASHVFGIRPLQQVLLPQQGGRLQAFTIAWDTTRKAWFSLHADGPVAPGDNL
                     HWSGRYQNWNLMCGECHTTAYRKGYDDEHDRYATTWAEANVGCQACHGAGRAHAESAR
                     RRAAAGERGQAPLPTPNRALAGAHAQVDQCATCHARRTRLVEDAVAGAPLFDQFIPDN
                     LRPGLYHADGQQLDEVFEYGSYRQSRMYQAGVACTDCHDPHRGRLRAEGNALCTACHN
                     PAPDRGRFPGLQAKDYDAPSHHFHRGGGAGSQCVDCHMPSRNYMVVHPRRDHAIRVPR
                     PDLGARTGAPDACTGCHADRGAEWAAAAIERHHGKRTHPPHYGEILAAARAGAPGAAA
                     GLAALARDAAVAAIVRASAIEQLAQLDPQAVPAAALRDPDPALRTSAAAAFALRPAEE
                     RLAQLAPLLADPVRAVRITAARALADLPATALAPELQDRQRAALAEFVAAQQAMADMP
                     NAQLNLAGLALAQRDPLVAEQRLRRALQREPALDSARLGLATLLAQTRREDEAERVLR
                     DGLARATSPGELHLALGLLAGQRQRWEEAARELRTADALLPGHARVRRNLDAVERYLE
                     RTRPR"
     gene            complement(27009..28757)
                     /locus_tag="PKN25_05125"
     CDS             complement(27009..28757)
                     /locus_tag="PKN25_05125"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNV90315.1"
                     /translation="MKAALATLLIPVATLAIGVQAGHAASPVQHDRPNILLIMADDLG
                     YTDLGSYGSEIATPNLDTLADTGVKMTQFYASPFCSPTRAMLMSGTDNHLAGFGDMAE
                     LMLPEQRGKPGYEGYLNERVVPMAQVLRDAGYRTLMTGKWHLGVPEQYSPAARGFDQS
                     YALVHGGSSHWSDGAGIVAADPAKPPKAIYRENGKETTLPKDFFSSDFFTSRLIEYID
                     AGKGSGKPFFAYLAFTAPHWPLHAHDADIARYEQRYKDGYDKLRRERLERMKKLGLVA
                     ADTPVFEGHPLWPKWDSLSAAEKESEARRMAVYAAMVDNMDQNIGRMLDYLKKTGQLD
                     NTFIFFLSDNGADGNSVYDVARTREWIHKDMDNSIAHIGKSGSYAEYGPGWAQVGSTP
                     FRMFKSFMYEGGIAVPAIAWGPGVKGGKLESAMAHVTDIAPTLFELAGAKHPGTEYQG
                     RPVLPLRGASMLPLLQGRGQAVHGADKAIGWELGGRKALRKGDWKIVSANQPWGTGDW
                     ELFNVAQDRSESRNLAAANPQKLGEMLVAWRDYVRETGTLEIPNLANRPGYSNGAKYY
                     EDLKYEATLVPRTAKP"
     gene            28997..>29219
                     /locus_tag="PKN25_05130"
     CDS             28997..>29219
                     /locus_tag="PKN25_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HNV90316.1"
                     /translation="MNQQALSALIWSVADLLRGDFKQSEYGRVILPFTVLRRLDCVLA
                     PTKAAVLAEHRDKEQAGLLYLVVEKFAHIE"
BASE COUNT         4094 a        10144 c        10352 g         4429 t
ORIGIN      
        1 cccttcggca tgccggtcga ggtgcagatc cgcacccgcg agatgcacca catcgccgag
       61 accggcgtgg cctcgcactg gctgtacaag gaagacgaga agaccctcac cgagctgcag
      121 cagaagacgc actcctggct gcagtccctg ctcgagctgc agtcggcctc gggcgaggcc
      181 accgagttcc tcgagcacgt caagatcgac ctcttccccg gcgaggtcta cgtgttctcg
      241 cccaagggca agatcttctc catgcccaag ggctcgaccc cggtcgactt cgcctacgcg
      301 gtgcataccg acgtcggcaa tcgctgcgtt gcctgccgca tcaacggcga cctgatgccg
      361 ctgcgcagcg agctgcgcaa tggcgaccag gtcgagatca tcaccgcggc ccacgccaac
      421 cccaacccgg cctggctgtc ctacgcgcgc accggcaagg cgcgcgcgca gatccgccac
      481 ttcatgaaga acgcgcagca ggacgagtcg gtcacgctcg gcgagcgcct gctcaaccag
      541 gcgctgcgcc cgcacgggct caccctcggg cagatctcca gcttcgcctg ggaccgcttc
      601 ctgcgcgacc gcggcgtgcg ccacaagaag gaggtcttct ccgacctcgg gctcggcaag
      661 cgcctgccgg tcatcgtcgc gcgccgcctc gccgaggcgc aggacctcga gtccggtcgc
      721 ccggacgtgg tcaagcccaa gccggcgggg gcgatcagca tccgcggctc ggaaggtgtg
      781 gcggtgcagc tcgcgcgctg ctgccatccc atcccggggg acccgatcat cggcaccctg
      841 cgcaagggcc agggcctcga agtccacacc cacgactgcc ccaacgtggc caagctgcgc
      901 ggcgaccgcg ggcgctgggt ggatgtcgag tgggagcaca tggtcgacga ccgcctgttc
      961 gacgtcggca tccgcgtgct cagccacaac gcctgcggcg tgctggcgcg ggtggcgagt
     1021 gcgatcgccg aggaggacag caacatccag agcgtcagca tggatggcgg acagggcgaa
     1081 tacaccgcgc tcaacttcac cgtgcaggtg cacgaccgca tgcacctggc gcgcgtgatg
     1141 cgcggcgtgc gcagggtgca ggaagtggtg cgcatcggac gcgtcaaggc cgacggccgc
     1201 gtcggctgag gaccccgccg tccgccgacg ctgctagaat gcgcgccgac cgcgacgcct
     1261 ctgtggcgcc gcacgtgaac ccacggggga cccgacatgg cgatgtacga atccgatcac
     1321 accaagttca tgcgcgagtg gatggagaag cacccggagc agcgcgacga gcagaaagag
     1381 ggccgtgccc tgtggtggga caagccgcag agcgtcgagg agcagaagcg cgtcgccgcc
     1441 gcccgcgtgc cggtcaagcc ctactactac gacatcaaca actgatccgg catgacgcgc
     1501 cgggggtgac gcggctgtgc tcgcgccgcc gcccgctccg gccgcggcgc ctcgtccctc
     1561 cgttgcaagc tcgcctccgg catcgcgtca ggccgacatg agcctgccga gtcgtctcgc
     1621 cttcatcgat gtcgagagca ccggcgcgca cccggtgcgc gaccgcatca ccgagatcgc
     1681 catcctgcgc gtcgaggacg gcgaggtcgt cgcgcgctgg gagagtctgg tcgcgcccgg
     1741 ccagcccatc ccgccgctga tcgaggaggt caccggcatc tccgacgcca tggtggccga
     1801 ggcgcccgcc ttcgaggtgt tggccgatac ggtcgccgcg ctgctcgagg gctgcgtctt
     1861 cgtcgcccac agcgcgcgct tcgactacgg cttcatccgc aacgccttca cccgcatcgg
     1921 tcgcgagttc gaggcgccgg tgctgtgcac cgtcaagctg tcgcgcgccc tctaccccga
     1981 acaccaccgc catggactgg atgcgctgat cgagcgccac ggcctcgtct gcgccgcgcg
     2041 ccaccgcgcg atgggcgacg tcgaggtgtt gtggcagttc gtgcgcgggc tggaggccgg
     2101 cttcgcgccc gaggtgctgg cgcgcgcagc cgcacgcgcg atgaaggcgc cgacgcagcc
     2161 gcccggcttg cccgaaggca tgctcgaggc cctgcccgac gcccccgggc tctatctttt
     2221 ccacgcccag cccgagccac cgcctgcggg cgcgcgcgac ccggcactct tcgtcgggcg
     2281 cgcgcagagt ctgcgcgcgc gggtgcgcga gcatttcggc gcgggtgcgc gcaaggggcg
     2341 cgacgccgag ctggcggcgc gggtgaagcg ggtggagtgg atcgagaccg ccggcgagct
     2401 cgacacctcg ctgcgcgaga acgccctgct gcgtgcgctg gcaccgccgt acaaccgtcc
     2461 gcaggagccc gccgaggccg ccttcgcgct gcgcctgctg cccaggcggc ggcgcgcgcc
     2521 gatctacgag acggtggcga tcgccggcac cgacccggcg gactggcacg acctccatgg
     2581 cgtgttccgc aaccgccgcg aggccgacaa cctgctgcgc gagctggcgc tgttgtaccg
     2641 gctgtgcccg cgccggctcg gcctcgaggg cggcagcggc ccctgcaccg cgcacgccgc
     2701 acggcgctgc gcgggcgtgt gcgcgcggcg cgagaccccc gaggagcacg acgcccgcct
     2761 cgccggcgcg ctcgccgggg tgggcgtcaa gccctggccg tgggcggggc cggtggcggt
     2821 ggaagaggtg cgcgcgcacg gcccgcaccg gctgcaggtg ttcgaccgct ggtgccacct
     2881 gggcagcgcg caggacgagg ccggcgcgct cgccctcgcc gcggcgccgc gcgccttcga
     2941 cgccgacgtc tggcgcatcc tcgcgcgctg gctggcggtg ccggcccatc tcgcgcaggt
     3001 gcgcgtgctc gccgcgcagg cgggcgaagg ggagcggggc ggttaagcgg cgcaggctgc
     3061 cgcggcggtg ccggccacga acgatgcgcc gggcgcggcc tagtgcgccg cgcagtcgcg
     3121 cagctgcgac agcttgctga gcaccatgcc gcggcggccg cgcggctgga tcatgccgtc
     3181 gcgggtgagc tgcgagagga tgcgcgacag cgtttccggg gtcaggttga gctgcgcggc
     3241 gatggtctgc ttgtcgatgt cgaggcggat ctcgcagcgc tcggcatctt ccggcgcgag
     3301 ccgggtcagg tagtgggcga cgcgctgcgt gccgctgagc tggaactgaa cctgcatggt
     3361 cgacatcaga tcgtagctgg cttccgcgag ccggttgctc atcgacaccg aaaaggcggt
     3421 cgagctcacc atcgcggtgc gcagcgcggc ggtcggcacc agcagcagct cgctcgttcc
     3481 gatcgcctgg gcgttggcca cgtgtgggcg gttgatcagc gcggcctcct cgccgaagaa
     3541 gctgccgggc tcggcgaggc ggacgatctt ctcgccgccc gagggcgaga ggacgaagca
     3601 tttgaccctt ccgtcgagca ccaggtagat accgcgcgga cagtcctcct gctgcacgag
     3661 gagttcgccg cggacggcgt ggatcggccg cgcggcggcg agcacgcgct cgcgggcgca
     3721 agggtcgagc atgtcgaagg gagcgaagcg ggcgagcagg tcgctggcgt cgggggcgag
     3781 ggaggggggc ctgcagctca tgcggatctc ctgcggacgt cggcgtggtt ttccagtcgg
     3841 tagactagcc gctggtttcc accctgcatg gcgtcgcgac ctgcagcggg gcgcgcgcgt
     3901 gtttttggca tacctccgaa gaggtatccg cggattttgg caagtcactg aaaagtagac
     3961 gcaaaggtcg agggttgcag gcgttgcagc cgaggatcga agtggaaggg gtatgtccac
     4021 gttgttgtcc cggatcgcgt ccggatggag atttcacatg tatctggcgg tcccccgcct
     4081 gctcgtgagg cgctcgatca tgctgctggt gtcggtcgcc ctcgtggcga tgacgctgat
     4141 cgggctggcg ggcatgagcg catccttcgt cgtggtggat cgcagccgcg acagcgtgca
     4201 ggcgatcgcc gtggccagca ccctgcgcgg ccatacccag cggatcgcca acctgatcgc
     4261 gatcgatgcg ctcaagggcc gcatcggtgt gtccgagcgc acgcgcgcgg cgatggccga
     4321 catcgagcgc gagctgcagc aggccgcgct gcgccgcttc gtggacgagg ctccgggaga
     4381 gctcttcgcg gcgacctacc gcggcgtgca ggcgggttgg gagcgctcgg tgcggccgcg
     4441 gatcgaggcg ctcgccgggc ctgtgccgcc ggatgcgcgc gaggtcgaga cgctgctcgc
     4501 cggggtcgac gacttcgtcg gtcaggtcga tagcctggtc gcggtgctcg gccaggagaa
     4561 cgagcgccgt atcgacgagc tgcgccgcat cctcgcgctc gccgcggcgg tgacgctggg
     4621 ggtggtgctg gtggtgatcg tcttgctgca gcgggcgctg ctgcgcccgc tcggtggcct
     4681 gctcgacgcc gcacgccgca tcgccggcgg tgatttcggc gtgcgcgtgc gctacaccgg
     4741 ggaggacgaa ctcggccagg tcggcagcgc cttcaacctg atggccgacg agctcgcccg
     4801 ccactatcac ctgctcgagc tgcgcgtcac cgagaagacc gccgagctgc agcgcagcaa
     4861 ccgttcgctc gagctgctct accacgccat cgcccggctc taccaggcgc cgagcgcgcc
     4921 cgacgcctac gaggccaccc tgcgcgacat cgaccgcgtc gccgggctgg agggctcctt
     4981 cgtctgcatc gagccgcgcc ccggcgcccc cgcggcggtg atcgcctcgt ccatggggcc
     5041 ctgtccggat cgcgccgagc gcggcgagga cgcctgcgcg gcctgccggg ccggctcgga
     5101 ggggcaggcg ctggcgctgc tgtcgccgac cctgctgcgc tttccgctgc gcgaccgcga
     5161 gcaccatcac ggcatgctgc gcctgtcgct gtccgacggc gcgcgcctgg aggactggca
     5221 gcgccagctc gtcgaggcgc tgtcgcgcca catcggcatg gcgctcggcg cggcacggcg
     5281 caccgagcag gagcgcctgc tcgcactgca ggaggagcgc tcggtgatcg cgcgcgagct
     5341 gcacgattcg ctcgcgcagg cgctgtccta catgaagatc caggtcagcc tgttgcagcg
     5401 cgcactcgcc gatccggcgc gcacggcgga ggcggagccg atcctggccg acctgcgcga
     5461 gggcatcagc gccgcctacc gtcagctgcg cgagctgctg gtgtccttcc gcctcggcct
     5521 gtcggccgac ctcgccaccc tgatggaaga tgcggcgcgc gaatacggca cgcgcggcgg
     5581 cctggaggtc gagctcgccg tggagctcgg cgcctgccag ctcagcccga accaggaggt
     5641 gcatgtgctg cagatcgtgc gcgaggcctt gtcgaacatg gtgcgccacg cttcggcacg
     5701 ccatgcctgg gtggcgctgc gtggcggcgc ggatggcgag gtgctgctgg aggtgcgcga
     5761 cgacggctgc ggcatcggcg cgccgccggc ggatgcccgc aaccaccacg ggctggcgat
     5821 catgcgcgag cgcgcgcgca gcatgggcgg ggaaatcgac atcgggccgg cgctgccgag
     5881 cggtacccgg gtgtgcgtgc gcttccgatc cgccaacgcg agcatggcgg cgatggcgca
     5941 gggtgatggg aagaaggaaa cggagacatg aacgagagct gtcgcgtgct gatcgtggac
     6001 gaccacccgc tgttccgacg cggactggtg cagctgctgc gcacgattcc cgccttcgtc
     6061 ctcgtcggcg aggccgccag tggcgcagaa gggctcgtgc tcgcgcgcga gctgcgcccc
     6121 gacctggtcc tgctcgacct caacatgcgc gacggcgacg ggctcgacat gctgcgcggc
     6181 ctgcgcgcgg cgcgcagcga ggcgcgcgtg gtgatggtca ccgtgtcgga ctccggcgag
     6241 gatgtggtgg ccgccttgcg cggcggcgcc gagggctacc tgctcaagga catggagccc
     6301 gaggccatgc tcgaggcgct gcaggccgtg gccgccgggc gggtggtgat ccccccgcag
     6361 ctcaaccacc tcctcgccgc cgcgttgcgc ggcgagtcgc ggccacaatc ggcgggcgcc
     6421 gccgggctca ccgagcagga gctgcgcatc ctcgagaaga tcgccgccgg gctccccaac
     6481 aagcagatcg ggcgcgagct cgacatcgcg gagggtacgg tgaaggtcca cgtcaagcac
     6541 atcctgcgca agctcgagct gcgctcgcgc gtcgaggccg cggtgtgggc ggtggagcat
     6601 ctgcgggcct gagcggcggg gaagggggcg cgcggcaagc ccggctgtat tgagatgagt
     6661 tcgcaggttg actcaggtca ataattgact gctgctactg ggctgtaatg gcggctgcgc
     6721 aatttgctgg atcgtccttg gcttcctccg catctccgcg cgcggcgagt tcccgccgga
     6781 acttcctctt cggacgcacg cgcgcagtcg agcccgcact ccgcccgccc tgggcgctgg
     6841 aggaggacga cttcgtcgcg cgttgcacgc gctgcgagcg ttgtatcgag gcctgtccca
     6901 ccaccatcct ggtgcgcatg gacggcggct tccccggggt cgatttcagc cgcggcgaat
     6961 gcagcttctg cggcgattgc gtcgccgcct gcgaacctcg cgcgctttgg cgcgaggacg
     7021 cgtccgctgc gccctgggat cgtgtcgccg tgatcggcgc agcctgcctg gcggcgcgcg
     7081 gcgtcgaatg ccgcgtgtgc ggcgaggcct gccccgaagg cgccatccgc ttccgtcccc
     7141 gcctcggggg cgtcgcccat cccgaactcg acgccgccgc gtgcaccgcc tgcggcgcct
     7201 gcatcggccc gtgcccgacg cgtgcgatcg acacgcgcgt gcgcaatccc ctccctacgg
     7261 agagcgcaac atgaatattg caagtctggt cgtgcgggcc tttcccgcgg atttccccga
     7321 gatcgtcgag ggcctgaaga aggtgcccgg ggtcgagttg cacgggcagt ccgccgagaa
     7381 gggcaacatc gtcatcacca tcgaggacgg cgcgggctgg tcggtgaccg actcgatcct
     7441 ggcggtgaac ctgctgccta aggtgcaggg gctgacgatc gcctacgaat acaccgatga
     7501 aggtctggaa ttgacggagg tctgacatga gcatgaatcg acgcgaattc atcaagaaca
     7561 acgcgatcgc cgcggcggcc gccaccgccg gcatcgggat cccggtcgtc gccgaggcgc
     7621 agcagcccgg cgcgggcggc accaaggtgc gctgggacaa ggcggcctgc cgcttctgcg
     7681 gcaccggctg ctcggtgctg gtgggcgtgc aggacgggcg cgtggtcgcc acccagggtg
     7741 atccggactc gccggtcaac cgcggcctga actgcatcaa gggctacttc ctgtccaaga
     7801 tcatgtacgg cgaggaccgc cttaccaagc cgctgctgcg catgaagaac ggccagttcg
     7861 acaagaacgg cgagttcacg ccgatctcgt gggagcaggc cttcgacatc atggagcaga
     7921 agtggaagaa ggcgatcaag gagcacggcc ccgactccgt cgccatgttc ggctcgggcc
     7981 agtggacggt gtgggagggc tatgccgcct cgaagctgat gaaggccggc ttccgcacca
     8041 acaacctcga tcccaacgcg cgccactgca tggcgtcggc ggtggccggc ttcatgcgca
     8101 ccttcggcat cgacgagccg atgggctgtt acgacgacat cgagaacgcc gacaccttcg
     8161 tgctgtgggg ctcgaacatg gcggagatgc accccatcct gtggagccgc atcaccgacc
     8221 gccgcctgtc caagcaaggc tgccaggtgc acgtgctgtc gaccttcgag catcgttcct
     8281 acgagctcgc cgacaacccg atgatcttcg tgccgcagac cgacctggcg atcctcaact
     8341 acatctgcaa ctacatcatc cagaacggca aggtgaacaa ggccttcgtc gagcgcaacg
     8401 tcaagttcaa gatcggcgag accgacatcg gtttcggcct gcgcccgaac catgtgctgg
     8461 aagccaaggc cacgagcaac ggctacccgg gtgccgaggg caagcccaag ggcgacaccg
     8521 gcgcggccaa ggacatcagc ttcgacgagt tcgccaggtt cgtctcggag tacaccgccg
     8581 agaaggtgtc gaagctttcg ggcgtgccgg aggatcgcct ggtcaagatg gccgagcagt
     8641 atgccgaccc gaaccgcaag gtggtgtcgt tctggaccat gggcttcaac cagcacaccc
     8701 gcggcacctg ggtgaacaac atgatctaca acgtccacct gctggtgggc aagatctccg
     8761 agcccggcaa cagcccgttc tcgctcaccg gtcagccctc ggcctgcggc accgcgcgcg
     8821 aggtcggcac cttcgcccac cgcctgccgg ccgacatggt ggtcaccaac ccgatgcacc
     8881 gcgcgctcac cgaggaattg tggaagctgc ccgagggcac gatcccgccc aagatcggtc
     8941 tgcatgcggt ggcgcagagc cgcgcgctca aggacggcaa gctcaagtgc tactggacca
     9001 cgaccaccaa caacatgcag gcgggcccca acgtcaatga cgagatctac ccgggctggc
     9061 gcaacccgga ggccttcgtg gtggtgtccg acgtctatcc gaccgtctcc gcgctgtcgg
     9121 ccgacctgat cctgccgtgc gcgatgtggg ccgagaagga aggcgccttc ggcaacgccg
     9181 agcgccgcac ccagttctgg cgccagcagg tgtcggcgcc gggcgaggcc aagtccgacc
     9241 tgtggcagta catccagttc gccaagcgct tcaaggtcga ggaagtgtgg ccggaggagc
     9301 tcgtcgccaa gaagcccgag taccgcggca agaccctgta cgacgtgctc tacgccaacg
     9361 gccaggtgaa caagttcccg ctcgccgacg tcgagaaggc caacgggcat gcgattccgg
     9421 gctacatgaa cgacgagtcg aaggagctcg gcttctacct gcagaagggc ttgttcgagg
     9481 agtacgccgc cttcggtcgc ggccacgccc acgacctcgc cgacttcgac gtctaccaca
     9541 aggcgcgcgg cctgcgctgg ccggtggtgg acaacaagga gaccctgtgg cgcttccgcg
     9601 agggctatga cccgtacgtg aaggcaggcg agggcgtgaa gttctacggc cacaaggaca
     9661 acaaggcggt cgtgttcgcg ctgccctacc aggatccgcc cgagatgccc gacgccgagt
     9721 tcgacatgtg gctgtgtacc ggccgcgtgc tggagcactg gcacaccggc tcgatgaccc
     9781 ggcgcgtgcc cgagctgcac cgctcggtgc ccgaggcaca gatcttcatg caccccgagg
     9841 acgcgcagaa gcgcgggctg cagcgcggaa tggcggtcaa ggtggtgtcg cggcgcggcg
     9901 agatcgtcgc ccgcctggag acccgcggcc gcaacaagcc gccggtcggg ctcatcttcg
     9961 tgcccttctt cgacgaaggc cggctggtga acaagctcac gctcgacgcc acctgcccga
    10021 tctcgaagga gaccgactac aagaaatgcg cggtgaaggt ccttaaggtc tgatgagagg
    10081 aggcgcgagt ccggcccccc gccgaccggg gggacgcgcg gagggcgggg ctcgcgatgc
    10141 tgatgaacga caaggtgcaa tcgaccggac gcgttgaacc cgtcgctccg gcttcgcgcc
    10201 gccgcttcct caaggatgcg gcgggcgtcg ccggcggtgc gggtctgctc gcgctcggtg
    10261 ccgggatgta cgcgaagcag gcgtcggcgt tgccggcgac ggccatccgt ccgcccggcg
    10321 ctctccccga agacgatttt ctcgcggcct gcgtacgctg cggcctgtgc gtgcgcgact
    10381 gtccctacaa gaccctgaag ctggccgagt tcggcgacgg ggtggcgacg ggcacgccgt
    10441 acttcgaggc gcgcgacatt ccctgcgaga tgtgcgagga catcccctgc gtggtggcct
    10501 gtccgaccgg cgcgctcgac cgtgcgctgg tcgatatcac caaggcgaag atggggctgg
    10561 cggtcctgat cgaccaggag aactgcctca acttcctcgg cctgcgctgt gacgtgtgct
    10621 accgggtgtg cccggtcatc gaccaggcga tcactctcga gcgcatgcac aacccgcgct
    10681 ccgaccgtca cgcgatgctg ctgccgaccg tgcattccga gcactgcacc ggctgcggca
    10741 agtgcgagaa ggcctgcgtg ctgcccggcg aggcggcgat caaggtgttg ccgatcaagc
    10801 tggcgcaagg ctcgaaggcc gaacactacc tgcgtggctg ggaagagaag gaggccgccg
    10861 gcaagtcgct catcggcgac caggtcgagc tgccggtgcg cggcctcgaa ggcaaggcct
    10921 acggcgacac tcgggtcgaa cccgggcaag ggccggtcca ggtgcccgcg caggtgccgg
    10981 attacgttcc cgcgaagccg gcgggcggtg gtctcgactc ggggtggaag ccatgagcgc
    11041 gcgactcggc gccggcggcg tgccggaacg caaggtcccg cccaccagcg tgcgcggcgc
    11101 cgcgatgcgc ggcgtggtcg cacgtcccgg gcgcgaggcg gtggaggcga agggctggct
    11161 gcgcgcgcac aagtggctgc tgctgcggcg cgcgtcgcag ctcggcatcc tcgcactctt
    11221 cctcgtcggg ccatggctgg ggtggtggat cgtcaagggc aacctgtcgt ccagcctcac
    11281 cctcggcgtg ctgccgctca ccgacccctt cctggtcgtg cagcagctcg ccgccgggca
    11341 ctggccctac cgggaggcgc tgctcggcgc cggcatcgtg ctcgccttct atctgctctt
    11401 cggtgggcgg ctgttctgct cgtgggtgtg tccggtcaac ctcgtcaccg acgccgccgc
    11461 ctggttgcgc cggcggctgg gcctcaaggg tggcaaggtg ccgtcgggca gcacgcgcta
    11521 ctggctgctc ggcttcgtgc tggtcatcgc ggcggcgacc ggctcgctgg cgtgggagtg
    11581 ggtgaatccg gtgtcgatgt tccaccgcgg cctgatcttc ggtttcggac tggcctgggg
    11641 catcgtcggc ggcatcttct tctacgacct gctcatcgcc ccgcgcggct ggtgtggcca
    11701 cctgtgcccg caaggcgcct tctacgccct gctcggacgc gccgccgtcg tcaaggtctc
    11761 ggccgccaag cgcagcgcgt gcaacgactg catggactgc ttcgccgtct gccccgagcc
    11821 ccaggtcatc cggcccgcgc tcaagggcgt aggccaggac caccccctca ttctggatgc
    11881 cgactgcacc acctgcggac gctgcgtgga tgtgtgcggc aaggacgttt tccgcattac
    11941 gacccgattc aacaggagcg agtcatgaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnna cccgaaatct ggtgctcgcg ctcgtcgccg ctgtcggctt cggtgccatg
    12121 gtgccgcccg cggcgatggc gcaggcggtg aagagcattc gcggcgccga cgtggccgag
    12181 caggagctgc aggtcggcaa ctacaagccg attcccgacc aggcgccgat cgagcgcgac
    12241 tacgtgcagc aaccgccgct gatcccgcac aaggtggagg gctacgaggt cacgatcaac
    12301 ttcaacaagt gcatggactg ccacgcctgg agccgctaca aggagtcggg cgccaccaag
    12361 gtcagcctga cccacttcaa ggatcgtgac ggcggcgaac tgtcgaacat ctcgccgcgc
    12421 cgctatttct gcatgcagtg ccatgtgccc cagaccgacg ccaagccgct ggtcggcaac
    12481 aagttccagc gcgcggaagg cctgcgctga agaatcgaca cacggggatc gacatgaccg
    12541 acaacaacaa gagcctgatg cagcgcatcc gcggtgcggg ctggggcgcc atcgtcctgc
    12601 tcgtcgccgc gggcgtggtg ttctgggggg gcttcaactg ggccctggag atgaccaaca
    12661 cggagaagtt ctgcatttcc tgtcatgaga tggagcagaa cgtcttcaag gagtatcgca
    12721 acaccatcca ctaccagaac cgcaccgggg tgcgcgcgac ctgcccggac tgccacgtgc
    12781 ccaaggagtg gggcccgaag atgatccgca agatcaaggc ctcgcgcgag ctctacggca
    12841 aggtgatggg caccatcgac acgccggaga agttcgccgc cgagcgcctg cgcctggcgc
    12901 agaacgagtg gaaccgcatg aaggcgaaca actcgcagga gtgccgcaac tgccacaact
    12961 acgagtattt cgactactcg gtgcagggcc gtcgctccaa ccagatgcac caggccggct
    13021 tcgccgaggg caagacctgc atcgactgcc acaagggcat cgcgcactcg ctgccgccgg
    13081 tcgaccagca catcggcgcg ccgcgcgaag gcgtcgctcc cgaggtgatg cacccgcccg
    13141 cgaagaagga ctgagctgcg ccgcagcccc cgccccgcgc tgcgccgcat cgagcggtgc
    13201 gccgcggggc gcgcctttcc ggtgtgtgcc gggatggcgc atcccgggcc gcctgtgttc
    13261 caatccgtcc ccatgctcga agtcgaaagt ctcgcctgcc tgaaaggcga tcgcctgctg
    13321 ttccgcgacc tcgccttccg cctccaggcg ggtggcctgc tgcgcgtcgc cgggcccaac
    13381 ggggtcggca agaccagcct gctgcgcctg gtcaccgggc tggcgctgcc cgaggccggc
    13441 gagatccgct ggcgcggcga gtcgatccgg cgcgcgcgcg aggccttcca cggcgacctg
    13501 ctctacctcg gccacgccgc cgcgctcaat gacctcctca gcccgctgga gaacctgcgt
    13561 ttcgcctgcg ctgccgccgg cgacgcggtc gacgcggagg actgcgtgca ggccctgcag
    13621 cgcatcggcc tcgccgacca gctcgacctg ccggcgcgcg tgctgtcgca gggccagcgc
    13681 cgccgcgtcg gcctggcgcg gctgttcctg tcgaacgcgc gcccgctgtg ggtgctcgac
    13741 gagcccttca ccgcgctcga cgtgcgtgcg gtcgccgatc tcgccgccac gctctcgcag
    13801 cactgcgaag cgggcggcat ggtgatgctc accacccacc aggacgcgcc cttcgcgcgc
    13861 ccgcccgagg tgctcgacgt cggagtcttc gcatgctgag caccttcctc gccgtgctgc
    13921 ggcgcgacct gctgctggcc tggcgcgggc gcgccgacgt gctcgtcacg ctcgccttct
    13981 tcatcatcgt ggtgtgcctg ttccccttcg gcgtcggcgc cgagcccaac cagctgcgcg
    14041 cgatcgcgcc cggcgtgctg tgggtcgcgg cgctgctcgc ctgcctgctt tcgctgcatc
    14101 gcctgttcgc gcaggactac agcgacggaa ctctcgagca gcttctcctg tctagcgagc
    14161 cggctgcgct gtgggtgatg gccaaggtgc tggcgttctg gctcagcacc ggcctgccgg
    14221 tggtggcggt ggcgccggcc atgggtctgc tgcttgacct cgaacaaggc gggctgcccg
    14281 tgctgatact aagtttgctg ctcggcacgc ccatcctcgc gctgctcggt gcggtcggcg
    14341 cggcgctgac cctcgggctg cgcggcggtg gcatgctgct ggccctgctg gtgctgccgc
    14401 tgttcgtccc ggtgctgatc ttcggtgccg gcgcggtcga ggccgagctg tcgggtagcg
    14461 gtgccgccgc ccatctgctg ctgctcggcg gtggtctggc gggggcgctc gcgctggcgc
    14521 cggtggcgtg cgcggcagcg ctcagaattt caaccgattg acgacgcagc cgcgccccca
    14581 ggcgggcgcg gcgggccgag aaagcgaagc ctcgaagcat gacgaatccc gaacgcggtc
    14641 gcagcctgct gcggtttgct gcaccgcaga acttctatcc cctcgccggt cgtctcgcgc
    14701 cctggttcgc cgcgagcgcc gtcgtcctca ccgcggtcgg gctgtggctc ggcttcttcg
    14761 tcgcccccac cgacgccacc caggggcagg tctatcgggt gatcttcatc cacgtgccgg
    14821 cggcctggat gtcgatgttc atctacctcg tcatggcctt ctggtcggcg gtcgggctgg
    14881 tcatgaacac ccgcctgtcc ttcgtgatga gccaggcgct cgcccccacc ggggcgatgt
    14941 tctgcttcgt cgcgctgtgg accggcgcgc tgtggggcaa gcccacctgg ggcgcctact
    15001 gggtgtggga tgcgcgcctg acctcgcagc tgctgctgct cttcctctac ttcggcttca
    15061 tcgcgctcac gcgcgcgatc gaggatccgc gccgcgccga ccgcgccggc gcaatcatcg
    15121 cgctggtggg cgcggtcaac gtgccgatca tctacttctc ggtgcagtgg tggaacacct
    15181 tgcaccaggg cgcctcggtg agcctcacca aggcgccgtc gatggcgacc accatgctca
    15241 ccggcatgct ggtgatggcg ctggcgtcgt gggcctacac cctcgccgtg gtgctgtggc
    15301 gggtgcggcc gatgatcctc gagcgcgagc gccataccga gtgggtcggc gccgagctcg
    15361 agcgcgccgc gaggctttcg caaaccgccg gagggcgtgc ctgatgcagc agtgggagag
    15421 ctggtccgcg ttctgggaca tgggcggcgc cgccttttac gtgtggggca gctacggcct
    15481 cgcgttcgcg ttgatcgcgc tggaactcgt gctcgtgttc cagcgtcgga aggacaccac
    15541 ccgtcgcctg ctgcgctggc gccgcgcggt gggcaaggat gcaggcaagc gtaacggcga
    15601 aggcacgatg cccgccatgg agtcggaaac atgaaagccc gtagcaaacg tctgatgctg
    15661 gtcggcggcg gggtggccct gctgttcgcc gcggtcgccc tggtgctgag cgccttccag
    15721 caaaacctgg tgttcttcca tacgccgacc gaggtctccg agggcaaggc gcccaccggc
    15781 aagaccttcc gcatcggcgg catggtcgag gacggctcga tccagcgcga ggccgacggt
    15841 gtcaccgtgc gcttcgcgat caccgacacc gccagggtca tcccggtgac ctacaagggc
    15901 acgctgcccg acctcttcaa ggagggcaag ggcgcggtgg tgcagggcaa gctcgagggc
    15961 ggcgtgttcc gcgccaccga agtgctcgcc aagcacgacg agaactacat gccgccggag
    16021 gccgcccacg ccgtggaaca ggcccagaag gccgcccaga ccttgagcca gtgacatgat
    16081 tcccgaactc ggacacttcg ccctcattct tgccctggtg ctgtcgctgg tgcaggcggt
    16141 cgtgccgctg gtcggcgcgc agcgcaacag ctcggcgctc atggcggtcg gccgccccgc
    16201 ggcgcagggg cagttcttct tcatcctgtt cagctacttc tgcctgacct gggccttcat
    16261 ccagagcgac ttctccgtcc agctcgcggc caccaactcg cacagcgcca ccccgctgat
    16321 gtacaagatc accggcgtgt ggggcaacca cgagggctcg ctgctgctgt gggcgatgtc
    16381 gctgtcgctg tggacggtcg cggtgacggt gttctcgcgc cacctgcccg acgccttcct
    16441 cgcccgcgtg ctgggcgtgc tcggtctggt cagcgccggc ttcatctcct tcaccctggt
    16501 cacctccaac cccttcgacc gcctgctgcc ggccgtcgcc gagggccgcg acctcaaccc
    16561 gctgctgcag gacccgggga tgatcatcca tccgccgctg ctctacatgg gctacgtcgg
    16621 cttctcggtg gccttcgcgt tcgcgatcgc ggcgctgctg tcgggacgga tggatgccgc
    16681 gtgggcacgc tggtcgcggc cgtggaccac gatcgcctgg gtcttcctca ccgccggcat
    16741 cgcggtcggc tcgggatggg cctactacga gctcggctgg gggggctggt ggttctggga
    16801 tccggtcgag aacgcctcct tcatgccctg gctgctcggc accgcgctga tgcactcgct
    16861 cgccgtcacc gagaagcgcg gcgccttccg cagctggacg gtgctgctgg cgatcggcgc
    16921 cttctcgctg tcgctgctcg gcaccttcct ggtgcgctcg ggcgtcatca ccagcgtgca
    16981 cgccttcgcc accgacccca ggcgcggcct ctacatcctc gccctgctcg tgatcgtgat
    17041 cggcttctcg ctgctgctgt acgcgatgcg tgcgaacaag ctcatgggcg gcggcagctt
    17101 cggcctggtg tcgcgcgaga ccgccctgct cgccaataac gtcctgctca ccgtggcctc
    17161 ggcctcggtg ctgctcggca cgctgtatcc gctcttcctc gacgccctcg gcctcggcaa
    17221 gatctcggtc gggccgccct acttcgaggc ggtgttcgtg ccgctgatga ccccggtcgt
    17281 ggtgctgatg gtgttcggcc ccttcctgcg ctggaaggac gacgagctcc tcgccgaggt
    17341 gcgccgcgtg gcgcccgcct tcgtcgccag cgtggcgatc ggcgtcggca gcgcgtgggc
    17401 ggtggatcac ctcacctggc gcaccgcgct cggcctctcg ctcgcggcct gggtggtgct
    17461 cgccagcctg caactgctcg tccgccgcct cggcgagcgc gccggggcct cggccggttc
    17521 gcgcctgcgt tcgatcaccg cgtcgtggtg gggaatgtgg ctcgcccact tcggcatcgg
    17581 catcttcatc atcggcgtca ccatggtggg cagcctggac cgccacctcg acgtcaagat
    17641 gaaggaaggc cagcacgccg agctcgccgg ctacactttc gtcttccgtg gcgcggtgga
    17701 tgccgacggc cccaactacg acgccgcgcg cggcaggatc gaactgctgc gcgacggccg
    17761 ctcgctcgac atgctcactc ccgagaagcg cgtctatctc gcccagggca tgccgatgac
    17821 cgaggcctcg ctcgacatcg gcgtgttccg cgacgtgtac gtctcgctcg gcgaacagct
    17881 cgaggacggc gcctggatcg tcagcctcta ctacaagccc ttcatcagct ggatctgggc
    17941 aggttgcgtg ttgatgggcc tcggaggcgt gttcgccgcc gccgaccgcc gctatcgccg
    18001 cctggccgcg cgcgacgccg ccgcggcggt gggcagtcgc gtcgccgcct gagttcgacc
    18061 tgggaaaacc atgaaagcca agtttctcgt accgctgctg ctgttcttcg gcctcgccgg
    18121 cttcctcgcc ttcggcctca ccctcaaccc gcgcgaggtg ccctcgccgc tgatcgacaa
    18181 gccggcgccg aacttccgcc tcgcccgtct cgaccagccc gagcagacct tcgcgctcga
    18241 ggagatgcgc ggccaggtct ggctgctcaa cgtgtgggcc tcgtggtgcg tggcctgccg
    18301 ccaggagcac ccggtgctgg tacgcatggc cgcgcagaag ctggtgccgg tggtcggcct
    18361 caactacaag gaagtgcgcg gcgacggcgc gatcagcacc cgcggcatgg cgctcgaggc
    18421 cgagaccacg atggcgatcg agcgcgcacg gcgctggctc gccgatcacg gcaaccccta
    18481 cctgctctcg gtgctcgaca tcgacggccg cgtcggcatc gacttcggcg tgtatggcgt
    18541 gcccgagacc ttcctgatcg accgcgaagg ccgcatccgc tacaagcaca tcggcccgat
    18601 cactcccgag tcgctgcaga cccagatcat gcccaaggtc gaggagctgc gtcgtgccgg
    18661 ctgatcccaa gctcgtcccg tccgggctcc acggcctgcg tcgcgtgctc ggcgtcggcg
    18721 cgctcgccgc gctgatcgcc gccccgctcc atgccgccac ggccgaaggc ggcccggcca
    18781 tcacccccgc cggcatcgcc accgcgagcg acccgggcgt cgccccggcg gtcgccgccg
    18841 atcccaagct cgaagccgac atgatggagc tgtcgcacaa gctgcgctgc ctcgtctgcc
    18901 agaaccagtc gatcgccgaa tccaacgcgc cgctcgcggt ggacctgcgc aaccaggtgc
    18961 gcgaacagct cgccgcgggc aaggacaagg acgacgtgat cgactacctc gtggagcgct
    19021 acggcgactt cgtgctctac gagccgccct tcaaggctgt gaccgggctg ctctgggtcg
    19081 ggccgttcgc cctgctgctg gggggcgccg gctggctggc ctggcgcctg cgccggcgcc
    19141 gcagcgagat cgccgccgag ggcgcgctca gcgccgcaga ccgcgagcgc gcgcgtgcgc
    19201 tgctcgccgg cggcggggac gccgcctcga atcccgcatc cccggagtcc cgcaagtgac
    19261 cgccttcctc gttttcgccg gcctgctcgt ggccggcgcg ttgctattga tcctgccgcc
    19321 gctgctcggc tacggccgcc gcagccaggc gcagatgcgc cagagcgcga tggtgctgac
    19381 cgtgctgcgc gagcagctcg ccgatctcga cgccgagctc gccgagggcc gcatcgacgc
    19441 cgcgacccat gcgaagagcc gcgaggagct cgagcgccgt gccctcgagg aggccgaggt
    19501 ggccgccgag acggccgaca gcgccgaccg ccggccgagc aaggcgtggg cgctcgccac
    19561 cggcctcgcc atccccgcgc ttgccgtcgg cacctacctg atgatcggcg agcccgccgc
    19621 gctcgacccg aagaacgtcg agggccagca gggcttcacg caggagcagg tcaccgagat
    19681 ggtgggcagc ctggagaagc acctcgccgc caaccccgac aacgccgagg gctgggcgat
    19741 gctggcgcgg acctacctgg tgctgaagga cttccccaag gctgccgccg cctacgaacg
    19801 cctcgacaag ctggtgccca acgaccccga cgtgctctcc gactgggccg acgtggtcgc
    19861 cgcgcagacc ggcaaggtcg ggagcgatgc cgaggcgctg gtgctgcgtg cgctcgaggc
    19921 ggaaccgaag catcccaagg cgctggcgct ggctggcacc gccgcctacc agcgcgacga
    19981 cttcgccgcc gccgccggct actgggagcg catcctcgcc ctgatccccc cgggcgacga
    20041 cgtcgcgcgc ggcgtgcgcg cgagcatcaa cgaggcgcgc gcgaaggccg gcatggcgcc
    20101 gctcgccacc aacgaaggca atgccgcgcc gccgccggcc tcgccgctca aggtcgccgg
    20161 ccgcctgcag gtcgagggcg cgctcgccgg ccaggtcgcg gccgaggacg cggtgttcgt
    20221 gttcgtgcgt ggcccggccg gcggcccccc gctggcggcg ctgcgcttca agggcagcga
    20281 gctgccggtg aatttcgact tcgacggcgc gaggctgatg atgggcgacg ccgcggtgcc
    20341 cgagcgcgtc gtgatcgcgg cgcgcgtctc caagggtggc gatgccaccg cacgccccgg
    20401 cgacctcgag ggcgtcagcg ctccggtggc gccggatgcc gaaggcgtga cggtgacgat
    20461 cgatcgggta cgggagtgag gcgtcgggtg cgaggcgtcg ggggcaagga gcaaaacctc
    20521 cgcaagctgc gctgagaggc ttcaggaagc cggtggacgc tcgatcgccc gccgcagctg
    20581 acgcctcgct ccctacgcct tgcctcccac gcctcgcgcg tccgcatact ccagccgcac
    20641 ccgcttcacg ttgcacagca gctcgtagct gatcgtgccc gcggcctggg cgatgtggtt
    20701 gatcggcacc tgcttgcccc atagctcgac ctggctgccc aggccggcct cgggcagcgc
    20761 ggtgaggtcg atcgtcagca tgtccatcga cacccggccg atgatccggc tcgcgcggcc
    20821 ctccaccgcc accggggtgc cgtcgggcac cacgcgcggg tagccgtcgg cgtagcccat
    20881 cgccaccagg ccgacccgcg tcgggcgttc ggcgacgaag cggccgccgt agccgagcgc
    20941 ctcgccgggc gcgatctcgc gcaccgccat caccgcgctc tccagcgtca tcaccgcgcg
    21001 caggccgttg ccctcggcgg gcatgggatc cgccccatag agcaggatgc ccgggcgcgc
    21061 ccagtcgcgg cgcgcctgcg gccaggccag gatcgctgcc gagttggcca cgctgcgcgc
    21121 gccgggcagg ccggcggtgg cggcctcgaa gcgctgcaac tgttccagcg tggtcccgag
    21181 ctcgggctcg tcggcgcgcg cgaagtgcgt catcagcacg atgcgtccga ccttgccgct
    21241 cgcctgcagg cgccgccagg cccgcgccac cgcgtcgccg acgaaaccgg cgcggttcat
    21301 gccgctattg accttcagcc acacatccac cggtgcggcg agcggcgtgc gctccagcat
    21361 gcacagctgc tcctcgtggt gcacgaccat ccacagccgc tggcgcgcca cctcggcgag
    21421 ctcgtcggcg gcgaacacgc cctcgagcag caggatgggg gcatcgatgc cgcccgcgcg
    21481 cagctccagc gcctcgccga ggaaggctac cgcgaagccg tcggcctcgt cgcgcaaggc
    21541 ctgcgcgcag cgcagcgcgc cgtggccata ggcgttggcc ttgaccaccg cgagcgcccg
    21601 cccgccgtgg cgctcgcgag cgagaagata gttgtggcgc agggcggaga ggtcgatgag
    21661 ggcgcgggcg ggacgcatgg cggggatcgg aaggggtgga aaggcaaaat ggtgcgggga
    21721 attcgccagc gccgcaatgc gcggcccgat cgcgactcgc gtcgctccca cagaggctcc
    21781 cgcgccgtgg aatcgcgact cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21901 nngtcgctcc cacgagggcc cgccacgctg ccggagccgc gccgtaggag cgacgcaagt
    21961 cgcgatcggg cgcgcggatc accgccgcag cacatggagc cgtggaatcg cgactcgcgt
    22021 cgctcctaca ggggccgccc acgctgccgg ggcactgtag gagcgacgcg agtcgcgatg
    22081 cagccccccg ggtacgcgcc gtggctcacg aagccgcgca agcgcgcttc agacggtgcc
    22141 gagcggacgc tggcgggcgc cggccaccgg tatgctgcgg tgctcggcgc cgtagcgcgc
    22201 gaggccgagg tcgtcgtggc ggatcgccgg ctggcggccg acgaccaggt cggcgacgag
    22261 tgcgccggcg ccgcaggaca tcgtccaccc cagggtgccg tggccggtgt tgaggaagag
    22321 ctcggggaag ggcgtggcgc cgatgatcgg ggtggaatcc ggcgtcatcg ggcgcaggcc
    22381 ggtccagaac tccgccgcct tcacgtcgcc gccaccgggg aagaggtcgt tcaccaccag
    22441 ctccagggtc tcgcgccggc gcgggttgag gcgcaggtcg aagccgccga gctcggccat
    22501 gccgccgacg cggatgcgct cgtcgaagcg cgtcaccgcg accttgtagg tctcgtcgag
    22561 cacggtggac accggcgcgc gcgcggcgtc gaccagcggc acggtcagcg agtagccctt
    22621 caccggatac accggcaggt ccagtccgag gctggcggcg agcgcgcgcg agtagctgcc
    22681 gaaggcgagc acgtagcggt cggcgaccag cgtgtgctcg acgccgtcgg caccggcgat
    22741 gcgcacgccc tcgatatggc cgccgcggcg cacgagttcg cgcacctcgg tgccgaagcg
    22801 gaacgtcacg ccggccgcct ccgccatcgc cgccaggcgg gtggtgaaga gctggcagtc
    22861 gccggtctcg tcgttgggca ggcgcaggcc gccggcgagc ttgccggggc gacgggccag
    22921 cgccggctcg gcgccggcga gcgcgtcgcc gtcgagcagc tcgtagggta cgccgcactc
    22981 ctccagcacc gcgatgtcgc gcgcggcggc gtcgagctgg gcctgggtgc ggaacagctg
    23041 cagcgtgccg cggctgcgct cctcgtaggc gatgccggtg tcggcgcgca gcgtgcgcag
    23101 gcagtcgcgg gcgtactcgg agacgcgcat catgcgttcc ttgttcaccg cgtagcgcgc
    23161 gctcgagcag ttggcgagca tctgcgccat ccagcgcagc tggaacaggc tgccgtcggc
    23221 gcggatcgcg agcggggcgt ggcgctggaa cagccacttc agcgccttca gcggaatgcc
    23281 gggcgcggcc cagggcgtgg agtagccggg cgagacctgg ccggcgttgg cgtagctggt
    23341 ctcgagcgcg gcgccgggct ggcggtcgat cacggtgacg cgcgcgccgc gttgggcgag
    23401 ataccacgcg gtggtggtgc cgatcacgcc gctgccgagt acgatcacgt tcatttcgcg
    23461 tctcctgcct gtattcgatg gaattgcggc agtctaatga ggccttgcta gcgaaagtca
    23521 ctaaactgat atcgccaatc agtgaagatc accaatccgg aggccgcctt gccggcggcg
    23581 cagtgaaatg cgcgacctcg accgtacaga cctgaaaata ctggatttgt tgcagaagaa
    23641 cgggcgcctg acgatgaccg agctcgccca ccaggtgggc ctgtcggcga ccccgtgcac
    23701 cgagcgcgtg cgccgcctgg agcgcgaggg cgtgatcacc ggctaccacg cccgcgtcga
    23761 cccgcgcgcg ctcggccgcc cgctgctggt cttcgtcgag ctcaagctcg ccgccaagtc
    23821 gaacgacgcc ttcgagcgcg tgaagaagga gctcgccttc gtccccgagg tcatggagtg
    23881 ccacctcgtg tcgggcgact tcgactacct gatcaaggcg cgcatttcgg agatgagcga
    23941 ctaccgtcgc ctgctcggca acatcctgct caagctgccc tcggcgaccg agtcgcgcag
    24001 ctacgtggtg atggaggagg tgaaggagag cctctatatt cctcccatgt catgagccgc
    24061 gggaggtccc ggatggacga caggagccgc cacgcatgag ttcggaacgt ggcacctcga
    24121 tccgtgccct cgaacgcggc atcgaggtgc tgcgcctgct cggcccccag gagggcagct
    24181 cgctgcgcgc gctccacgcc gccagcgcgc tccccaagcc gaccctgctg cgcatcctgc
    24241 acaccctgga gcggggcggg ctggcgtggc gatcgatcga cgacgggctg tggcggcgct
    24301 cgctggcgtg gaccgcggcg ccgagcgggc acgcgatcga tccgcgcctg gtcgaggccg
    24361 cggcgccgca cctggtggcg ctgcagcgca cggtgctgtg gccgtccgac ctgctggtgt
    24421 accgcgactt cgcgatggag gtcgcggaga gctcccgccg cctctccggg ctggcgctca
    24481 atccgcgcta ccgcatcggc taccgggtgg acatcctgct ctccgcgccc gggcgggcct
    24541 ggcttgcctt ctgcgctgcg gccgaacgcg aagcggtgct cgcccactgg cgcgccgcct
    24601 cgccgtcgaa tccgcgcacg gcggcggtcc tcgagggcga gatcgacgcc ctcctggagg
    24661 agacgcgaca gcgcggctat gcggtgcgcg acgcgctctt cggcggctcg ttcgaggaca
    24721 tggcgggctt cgacgacggc ttcgacgcca tcgccgtgcc gatcctcgcc gatggcgggg
    24781 tgcgcggggc gatcaacctg gtgtggccgc ggcgctacga cctgcgcgcg aaggtggtgg
    24841 aggcgcatct ggccgacctg caggcctgcg ccgcagcgat cgcggccgcg ctcgcgtcgc
    24901 cgcgccagcc ctgagcaccg ccgcgccgtc gcggagccct tcagcgcggg cgggtgcgct
    24961 ccaggtagcg ttccacggcg tcgaggttgc ggcgcacgcg cgcatggccc ggcagcaggg
    25021 cgtcggcggt gcgcagctcg cgcgcggcct cctcccagcg ctggcgctgg ccggcgagca
    25081 ggccgagggc gaggtggagc tcgccgggac tggtggcgcg ggcgagcccg tcgcgcagca
    25141 cgcgctcggc ctcgtcctcg cggcgggtct gcgcgagcag cgtggccagg ccgaggcgtg
    25201 cgctgtcgag cgcgggttcg cgctgcagcg cgcggcgcag gcgctgctcc gccacgagcg
    25261 gatcgcgttg cgcgagcgcc agcccggcga ggttgagctg ggcgttcggc atgtctgcca
    25321 tcgcctgctg tgccgcgacg aactcggcca gcgcggcgcg ctggcggtcc tgtagctcgg
    25381 gcgcgagcgc ggttgcgggc aggtcggcga gcgcgcgcgc ggcggtgatg cgcaccgcgc
    25441 gcacggggtc ggcgagcagg ggggcgagct gcgcgaggcg ctcttcggcg gggcgcagcg
    25501 cgaaggcggc cgcggcgctg gtgcgcagcg cggggtcggg gtcgcgcagc gcggcggcgg
    25561 gcacggcctg ggggtcgagc tgggcgagct gctcgatcgc gctcgcgcgc acgatcgcgg
    25621 ccacggcggc gtcgcgggcg agcgcggcca ggcccgcggc cgcgccgggg gcgcccgcgc
    25681 gtgcggcggc gaggatctcg ccgtagtggg gtggatgcgt gcgcttgccg tggtggcgct
    25741 cgatcgccgc ggcggcccac tcggcgccgc ggtcggcgtg gcagccggtg caggcgtcgg
    25801 gcgcgccggt gcgcgcgccc aggtcggggc gcggcacccg gatggcgtgg tcgcggcgcg
    25861 gatgcacgac catgtagttg cggctcggca tgtggcagtc gacgcactgg ctgccggcgc
    25921 cgccgccgcg gtggaagtga tgcgagggcg cgtcgtagtc cttcgcctgc aggccgggga
    25981 agcgaccgcg gtcgggcgcg ggattgtggc aggcggtgca cagcgcgttg ccttcggcgc
    26041 gcaggcgacc gcggtgaggg tcgtggcagt cggtgcaggc cacgcccgcc tggtacatgc
    26101 ggctctggcg gtaggagccg tactcgaaga cctcgtcgag ctgctggccg tcggcgtggt
    26161 agaggccggg gcgcaggttg tcggggatga actggtcgaa caggggcgcg ccggccacgg
    26221 cgtcctcgac cagccgggtg cggcgcgcgt ggcaggtggc gcactggtcg acctgggcgt
    26281 gcgcgccggc gagcgcccgg ttcggggtgg gcagcggcgc ctggccgcgc tcgcccgcgg
    26341 cggcgcgcct gcgtgcgctc tcggcgtgcg cgcggccggc gccgtggcag gcctggcagc
    26401 ccacgttcgc ctcggcccag gtggtggcgt agcggtcgtg ctcgtcgtcg tagcccttgc
    26461 ggtaggcggt ggtgtggcac tcgccgcaca tcaggttcca gttctggtag cgcccgctcc
    26521 agtgcaggtt gtcgcccggg gcgaccgggc cgtcggcgtg cagcgagaac caggccttgc
    26581 gcgtggtatc ccaggcgatc gtgaaggcct gcaggcgtcc gccctgctgc ggcagcagca
    26641 cctgctgcag cggccggatg ccgaagacgt ggctggccgc gagcgcctgc gtgcggccgt
    26701 cgggcccttg cgcggcgacc atgaagtcgc cgccgcggcg gtagaagcgc gccgccgccg
    26761 caccctcgcc gaagcggact tcgccggagc cggcctcacc gaagtcgccg agcacggtct
    26821 gcgccgtcgc cggctgcatc gcatggcggt gcttcgattc ggcccaggcg tccgcctggt
    26881 cggggtggca ggcgaggcag tcgcggtcgg cgacgtggtc gggccgcgcc cccgcctgcg
    26941 gggcggccgc gaacgcggcc ggcaccatca ggacgcaggc gaggagcagg gcgggcaggc
    27001 gcatgcgctc agggcttggc cgtgcgcggg acgagggtgg cctcgtactt caggtcctcg
    27061 tagtacttcg cgccgttgct gtagccgggg cggttggcga ggttggggat ctccagcgtg
    27121 ccggtctcgc gcacgtagtc gcgccaggcc accagcatct cgcccagctt ctgcgggttg
    27181 gcggcggcga ggttgcggct ctcgctgcgg tcctgcgcga cgttgaagag ctcccagtcg
    27241 cccgtgcccc agggctggtt cgccgacacg atcttccagt cgcccttgcg cagcgccttg
    27301 cgcccgccca gctcccagcc gatcgccttg tcggcgccat gcacggcctg cccgcgcccc
    27361 tgcagcagcg gcagcatcga ggcgccgcgc agcggcagca cgggcctgcc ctggtactcg
    27421 gtgccggggt gcttcgcgcc ggcgagctcg aacagcgtgg gcgcgatgtc ggtcacgtgg
    27481 gccatcgccg actcgagctt gccgcccttg acgcccgggc cccaggcgat cgccggcacg
    27541 gcgatgccgc cctcgtacat gaaggacttg aacatgcgga acggcgtcga acccacctgc
    27601 gcccagcccg gcccgtactc ggcgtaggag ccggacttgc cgatgtgcgc gatgctgttg
    27661 tccatgtcct tgtggatcca ctcgcgggtg cgcgccacgt cgtacaccga gttgccgtcg
    27721 gcgccgttgt cggacaggaa gaagatgaag gtgttgtcga gctgcccggt cttcttcagg
    27781 tagtcgagca tgcggccgat gttctggtcc atgttgtcga ccatcgcggc gtacaccgcc
    27841 atgcgcctgg cctcggattc cttctccgcc gcgctcaggc tgtcccactt cggccacagc
    27901 ggatggcctt cgaacaccgg cgtgtcggcg gccaccaggc cgagcttctt catgcgctcg
    27961 aggcgctcgc ggcgcagctt gtcgtagccg tccttgtagc gctgctcgta cctggcgatg
    28021 tcggcgtcgt gcgcgtgcag cggccagtgc ggcgcggtga aggcgaggta ggcgaagaag
    28081 ggcttgcccg aacccttgcc ggcgtcgatg tactcgatca gccgcgaggt gaagaagtcg
    28141 gacgagaaga agtccttcgg cagcgtcgtc tccttgccgt tctcgcggta gatggccttc
    28201 ggcggcttgg ccggatcggc ggcgacgatg cccgcgccgt cgctccagtg gctggagccg
    28261 ccatgcacca gcgcatacga ctggtcgaag ccgcgcgcgg ccgggctgta ctgctcgggc
    28321 acgccgaggt gccacttgcc ggtcatcagc gtgcgatagc cggcgtcgcg cagcacctgc
    28381 gccatcggca ccacgcgctc gttgaggtag ccctcgtagc ccggcttgcc gcgctgctcg
    28441 ggcagcatca gctcggccat gtcgccgaag ccggcgaggt ggttgtcggt gccggacatc
    28501 agcatcgcgc gcgtcggcga gcagaacggc gaggcgtaga actgggtcat cttgaccccg
    28561 gtgtcggcca gggtgtcgag gttgggagtg gcgatttcgc tgccgtagct gccgaggtcg
    28621 gtgtagccga ggtcgtcggc catgatcagc aggatgttgg ggcggtcgtg ctggaccggc
    28681 gaggcggcgt ggccggcctg gacgccgatg gcgagcgttg ccaccgggat cagcagggtc
    28741 gcgagcgctg ctttcattgt ctgtctcctg gaataagccc ggcttctggc cggttccatg
    28801 aatttaattc aattctcgag aaaagctgcg cgatctgaaa atgttccgca gggtggaacg
    28861 tcggaccaag actggacggc agttctgccg cccgccggat gtccggcccg gtgtcccgtg
    28921 cgaggcagtc gagcgtgggc cggccaatcg tcttgcccac aatcgcaggc gatccccgcc
    28981 cgacgaccca gaccccatga accagcaagc cctctccgcc ctcatctggt cggtcgcaga
    29041 cctcttgcgc ggcgacttca agcagtccga atacggccgc gtgatcctgc ccttcaccgt
    29101 gctgcgccgg ctcgactgcg tgctggcgcc gaccaaggcc gcggtgctcg ccgaacaccg
    29161 cgacaaggag caggccgggc tgctctacct ggtggtggaa aagttcgccc acatcgagc
//
LOCUS       DAOQBS010000027        28944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_974_length_28944_cov_16.800443, whole genome shotgun sequence.
ACCESSION   DAOQBS010000027 DAOQBS010000000
VERSION     DAOQBS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 28944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28944
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_974_length_28944_cov_16.800443"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1385)
                     /locus_tag="PKN25_05135"
     CDS             complement(72..1385)
                     /locus_tag="PKN25_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HNV90317.1"
                     /translation="MPETVDPGAASCPALFVAAPASGQGKTTVTAALARLHTRLGRRV
                     RVFKCGPDFLDPQIHAVASGAPVHNVDLGMCGEADIARRLHAAAREADLILVEGVMGL
                     YDGAPSGADIARRFGIPVMAVIDARAMAQTFGAVAYGLAHYQPGLPFAGVLANHVGSA
                     RHADMLRESLPAGMRWFGAVMRDVDAALPERHLGLLQAAEIDDLGARLDRLADHLERT
                     GAAELPPTVAFPVVAVAEIPPLLAGKRIAIAHDAAYGFLYPANVDTLRALGAELAFFS
                     PLAGEGLPAQCDAVWLPGGYPELHGAALAANAGLWVGLRAHVAAGKPVLAECGGLMSL
                     LDAIVDKVGVEHGFAGLLPGRAVMQKRLAALGMQEVDLPGGRLTGHTFHYSRTETPLE
                     PWLRACRPDGGEGEAIYRRGPITASYVHFWFPSNPVAVAGLFGGG"
     gene            complement(1400..2248)
                     /gene="cobA"
                     /locus_tag="PKN25_05140"
     CDS             complement(1400..2248)
                     /gene="cobA"
                     /locus_tag="PKN25_05140"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNV90318.1"
                     /translation="MAVPDLAVHRPGPLPGWVYLVGAGPGDPELFTLRGARLVAGADV
                     IVYDNLVAPPILDLAPVEAERIYVGKKAADHSLPQDEINQLLLELARRNKRVVRLKGG
                     DPFIFGRGGEEMELLVEAGIHVEVVPGVTAAAGVSAYAGIPLTHREHAQSVVFATGFL
                     KEGKVDLDWPMLARPHQTVVIYMGISRLADICAQLVAHGLPPTTPAGVIERGTTHAQR
                     VVVADLATLAQRVLDEGVRPPSLTVVGSVVGLYPKLAWFEPQEQPKASPLPHAGCAVV
                     HGKGGE"
     gene            complement(2251..2859)
                     /gene="cobO"
                     /locus_tag="PKN25_05145"
     CDS             complement(2251..2859)
                     /gene="cobO"
                     /locus_tag="PKN25_05145"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNV90319.1"
                     /translation="MTQTPDRDTRHNARMARKKDVVDEKIAAAQAERGVLLVNTGNGK
                     GKSSAGFGLVARALGHGMQVGVVQFIKGRSDTGEEAFFRTQPGVRWHVMGEGFTWETQ
                     DRARDVASAEAAWALAREMLQNPEVGLVVLDELNIALKYQYLDVERVLADLRARPPMQ
                     HVVVTGRGAPPALVEAADTVTDMTPVKHAFAAGVKAMPGMEW"
     gene            complement(2856..3014)
                     /locus_tag="PKN25_05150"
     CDS             complement(2856..3014)
                     /locus_tag="PKN25_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90320.1"
                     /translation="MEDVVEAVLAQLDGLAAGLTAAFPEDVFTTIAAGMRASARRLHN
                     ENRTTGPT"
     gene            complement(3078..3317)
                     /locus_tag="PKN25_05155"
     CDS             complement(3078..3317)
                     /locus_tag="PKN25_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV90321.1"
                     /translation="MPRLYSPEQAAELLVGRRKALGLSQAEVAARLGISQNRLSELET
                     RPERLTFDRLLALAGVLGLELTLGDRKPAEVEREW"
     gene            complement(3426..4235)
                     /locus_tag="PKN25_05160"
     CDS             complement(3426..4235)
                     /locus_tag="PKN25_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90322.1"
                     /translation="MNAARRALPVDDAPLLEAERLALEVGERWLCCEFSLSLAAGECL
                     VLLGPNGAGKTTLLHTLAGLREPTVGEVRIGGLPYAAWPALEAARFRGLLAQQQPDPF
                     AASVLETVLVGRHPHLGRWGWEGPQDVAIARAALESVGLAGFEQRDVQTLSGGERQRV
                     GIATLLAQAPRLFLLDEPLNHLDLHHQIAALELFQRLLREQGTARGSVLVLHDINLAA
                     RYADHIILLDGRGGVIAGDRDAVLRADLLGQAFGHPLRRIEHEGRVVFLPD"
     gene            complement(4232..5218)
                     /locus_tag="PKN25_05165"
     CDS             complement(4232..5218)
                     /locus_tag="PKN25_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNV90323.1"
                     /translation="MRRRRAPLAIAAVLLSAAAMALLWALVAGDLPVAATDVFAALAG
                     AEGMGATVIRELRLPRALAAFACGGLLATAGALMQVLLRNPLADPYILGISGGAAVGA
                     LGAMMLGAALFWVDVSAFAGALAAMLLVFGLAHGDGSWTQTRLLLTGVIVAAGCGAAV
                     SLMLALAPDTRVMSMLFWMMGDASAAARPWPALAVLALALVAVMPYARDLNVLARGEL
                     SARALGVRVGHLRVALYVLASLLTATAVTLVGSVGFVGLVVPHLVRLALGNDQRVLIP
                     AAALAGGTLLTLADTAARTVMAPQQLPVGVLTALIGVPVFLFLLSRNPRGTP"
     gene            complement(5270..7108)
                     /locus_tag="PKN25_05170"
     CDS             complement(5270..7108)
                     /locus_tag="PKN25_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV90324.1"
                     /translation="MKIRVSALASACALACFLAPAAHAADAQLGHVVVTATRQPISAD
                     AALASVDVIERDEIARAGHSSLLGLLSSRPGVQMARNGGPGSSGSIFIRGANSGHTLV
                     LVDGVRMGSATSGSPVLETIPLELIERIEILRGPGSALYGADALGGVIQVFTRKGREG
                     FQPSVRVGAGTDGAREASATLAGGNERLRYSLTAGHERSDGFNARPNSATAADADDDG
                     FREDYLGASLVLDLGGNDELGANLLYSDMRNWYDAAQPFDSYLDKRAETFGAYLRKQH
                     SADWASTLRFGHGVDALDNQSNASTRSRFDTTQRQLSWQHDVAVGGGSLMAAYEFLQQ
                     RVKTTSDFEKTRRHINAFLLGWGGEFDRHNVQLNARHDRNSQFGGKTTGAAAYGYRLA
                     PEWRAHASIGTAFKAPTFNDLYFPVECYGAWGCFGGNPDLEPEEALNRELGVAWERNG
                     VGVDLTYFNNRIKNLIDWSTGIASNVGRADIQGIEAALSATLGDYRLRASVDLLDAED
                     DETGDQLGRRARVGGALALERAVGAWTWGVEWNGKGRRYDQVPNAVSNRLGGYGLVDA
                     YAHYAVARDWSVEVRANNLLDKDHELAKGFATQGRSAFVALRYAMH"
     regulatory      complement(7242..7478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7572..8120
                     /gene="cobU"
                     /locus_tag="PKN25_05175"
     CDS             7572..8120
                     /gene="cobU"
                     /locus_tag="PKN25_05175"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNV90325.1"
                     /translation="MTCELILGGARSGKSREAERRAVETGLAVTVIATAEALDEEMAA
                     RIRRHQADRPAGWRTVEAPLALADTLRREAAAERCLIVDCLTLWLSNLLADAHILPAG
                     ATAENLPRFRHERDALLAVLPTLPGRIVLVANEVGLGLVPETPLGRLFRDEAGRLNQM
                     VASACRRVVFVAAGLPLVLKEG"
     gene            8183..8785
                     /locus_tag="PKN25_05180"
     CDS             8183..8785
                     /locus_tag="PKN25_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNV90326.1"
                     /translation="MTLEDFLAWEAEQPERWEFFNGEAFMMAGGSDVHNVVTGNMYMA
                     LRIGLQHTRCNVFMSDVRLRLAESDDLFYPDVFVTCTDADRARRQVKEDPILIAEVLS
                     PSTEAYDRGDKFAAYRRFAGLKTVLFLSQDRAHVECFTRGEDGRWVLSEASGETARLA
                     LPAFDFELALADLYRDLPDADASDPAPIPPPSTPPPPPAA"
     gene            8782..9843
                     /gene="cobT"
                     /locus_tag="PKN25_05185"
     CDS             8782..9843
                     /gene="cobT"
                     /locus_tag="PKN25_05185"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNV90327.1"
                     /translation="MKPYAIASPDPLLSAAIQHKIDRKTKPRGALGRLEALAHQIALL
                     QQTLEPELRQPHIVVFAGDHGAARAGVSAYPQDVTWQMVENFLAGGAAINVFTRQLGL
                     DLTVVDAGVNHDFGKRPGLIDAKIAPGTANYIEEPAMSAAQRDAALERGRGVAHALAD
                     NGCNCIGLGEMGIGNTASASLLTHCLVGAEHDAELYTVTGRGTGLDDPGLFRKRSMLA
                     QALQRGGRPHDPLEVLVQYGGLEIAMMAGAILGAAERRMLVLVDGFIVTSAVLVAHAL
                     APAVLSCCVFAHRSQEPGHRVQLDFLQAEPLMELDLRLGEGTGAALAFPLVQAAVNFI
                     NEMASFESAGVSDQARRPG"
     gene            9843..10622
                     /locus_tag="PKN25_05190"
     CDS             9843..10622
                     /locus_tag="PKN25_05190"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNV90328.1"
                     /translation="MRDQLELFFTALGFFTRLPVPAWVPWSSERMHHAARYFALVGWV
                     VGGAGALGYLALAWLLPPAVAVVLSMALTIRLTGAFHEDGFADSCDGLGGGWDKAQIL
                     AIMKDSRIGSYGTIGMVLMLLAKAAALVELASPAVQGAGVALALLVAHPLSRLAATVV
                     LHRLPYVRADDSAKSVAVARPLAPAGLALAVASGLLPLLLLPPAAALCALLAAAAVTT
                     WSARLFQRRLGGYTGDLLGATQQASELAIYLALLAASRLAS"
     gene            10840..26868
                     /locus_tag="PKN25_05195"
     CDS             10840..26868
                     /locus_tag="PKN25_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukotoxin LktA family filamentous adhesin"
                     /protein_id="HNV90329.1"
                     /translation="MHHHREPEPVAAAPRLKEIVVHVTCALIVSHGLVPELAWAVDIV
                     PDGRTATSVSPLGTNAADVRNTDRLLGNGNAVNSFSRFNVGSGETANLHLPDGAAALI
                     NLVRDQKTTIDGMLNAIKEGRIGGKVYFANPHGVVVGPSGKVNVGSLHVSTPSQRFVD
                     DFFDAPGGAEASAAALVEGRAPRNTAAEIRIDGEVNATEQVSLSAGSITVGGKVLSGA
                     KFAGGAPDFSDVVNVHGLATGGAVVENQGRIEIVGDSGIELTGTVSSRRVNVGDDASL
                     RSGVSIAASGDIALRAPTIRIGSGGKLLAAGSDTHAGGDVTLQAQAVGGTLDALSDTA
                     ARIEASGATLTGRDLHLQASAEHRSTLAPLVTKTTSATIEVDGSTLEASGRATLEATA
                     TAAIATPDVVPLAVVDVDVAASVRVQGDTRITSAGPARLGATTTTTIDARPGLPDLGA
                     LPADAGAAIVIADTTASVDLLGNAELASTADKTELVASNRTTVDAAVDASAAGSSAVG
                     GSVAVSVVRSTTRARTADNARLAGKTGVGVHAQSDTRVTTSAKAAAGGASPQPAGTSK
                     TEETLADYEDEASTSDGSVSVAAAVAVSDLESTTLAALDATRAATSEGMLAVGSEALT
                     QADVVADGSSTGAAVGVGAAVALNLARTSAEARIGGSADAQGIEVVATLPTGDEINGF
                     TTAATSGAGASDVGVAGALALNVVDAGSRARITGSADAKGGTVRIGSDHRSESSASAT
                     PAEDATASGDTLGIGASVAVNVVANESRAELANGARLINPGAVTVEAVGDYLVHTEAE
                     AGAEGGIAATPVAAVAVVDNQTHAVIGSGERVQAGAKVGVTATHSSTVETSAKGSAEG
                     EKAAIGIAVAVNVLTERVSASIQRDVFTPGAIEVAAHNAHKGSATASASAKGGKGEEE
                     DGSSAEDGVDLEVQRQSGFAQDKQKDGSASKTQQPGSAESSEGKLSVAAAVAVNALDS
                     RSEAYVASAIELQAGGRLAVEAWSNTDAEAEADGSAAGSTATVGIGAAAAVNSIETHT
                     LAWIGDAQVTANGIAVNAGTEILGDDEENRFSASAKAGAGAGKVGIAGALALNLLETH
                     TRAEVLEGARADAEGGDVELEADSTTETSATALPDEPVDGGDFGVGASVALNLFREDE
                     VRARIRRGAEITDLDKLRVIANASSDTRATAEAGAAGSVALDAAVALSDLKLNTAAVV
                     EAGTEIVAAGAVEIRATSTGEHEAEATGDTESEKVGIGASGAIVLSTTTTTASLQRSL
                     QTTTSGEEGNLTIDADATRSYEAVAKASAAGGKAEEDTTAAERDKASTSSTLKDNQDA
                     QEGTQTTGNSNKLAVAAAVGAVVMDDDVRADVGRAQGADSDPAVRLDVGGELAIGAHN
                     QSDFSARGLGNTLDVSKLESSAQVGIGVGVGLAIARNDTAATIGDGTLVADAAGVQVA
                     AGSSQNTSAEFANKLAAEGVAGAGAEKVGIAGALAVAWSQSTTSASLGEGVRIGDRTT
                     GEYAGEVSITADNTSKLAAKAWSAALAGKVGVGASIAILRANNSQHAWIGEQAEVTAA
                     GLQLAGRNHLLNSSPSFDWTILDDLENRFTEANLQVLLGESNYYTETIAGAGGEQVAV
                     TGSFSVNVFDERTEATIGDGARVNTSGTVDLEADNQSSATAFAGGVSAAGKVGVGLAS
                     ADIVNRSQTRATIGADARIEQSAAIDIDATAAMDLAAVTASAAAAGKAGVGGVLSLID
                     SENQVEAATGARSFLRSDGAIDVDAGNTFDALGVAGVAGVGGTAGVGVSAGTFLVDNQ
                     TRASIGAAADVAAAGAIGVRGTAVQDVTSVVVGGAGGGTAGVAASAAVNDYNPLTYAW
                     IGDGARINQGVSGAALAGRSLAVHAHARTDLLSIVGTVGIGGTAGVGGAADVVVIDKE
                     TAAWIGEGVIARSGDSLAVGASSAEAIRSVGVGLSAGGTAGVQGSASVLVLDIDTLAS
                     VKTGSALFSDGNLVIAADADSDLDLLAGAIGAAGTAAVGAGAAVAVVDKTTHAWIDDG
                     AAASALGNAAAAQVADGGFDIDYVATAAGEGEVAAPGITPSDGENDLAGGSAALDATR
                     TSSASTSAHRGLAVTAVNRDDIKGFAVTGAAAGTAAVTLSGEVSVHQTDTRASIGSIA
                     LAEGASHTGVQVNADNTGANAAQSVRVAAGNDSFHLGIAGALSASGTVGVGVGADVAA
                     MRHATHAAIVEQSDVKARRDVEVAASSGQEVVSISASLGASGTVGVAGSVSVLSFDNA
                     TSASIGDEAEVAAGGNVSVKADDHTATTLVAGTIALGIGGGGVGAGVGVTLIEKDTRA
                     WIGAGAVVDALGKDTADLVVRSGESVDETATARGVQVQAESSEDLFTVSAAAAGGLYL
                     GFAGAVSVATVDSDTAAWIGADARVNRGGGDADAAQDVNVGARNELALFNVSGALGVG
                     AAGIAGGVDVGVIRNDTTAWIGDRAEVGAKRDIGVSALANTDIESYVVSAAGGIGALA
                     GGVGVYSVGGGLGDEAQGRLESGDDDAGSYVDGQATDGSVATLLGSYTDSRIGEAADR
                     VEDERGTLAASGRFTAAETRTLPAGTAAFVGENADLEAGRDIDLDARTDVEFDALDGA
                     LAIGAIGLGAGVGVADFDNDTLASVGRGSTLRAGAAGSVSLTAALDEDVDVTAFAGTG
                     GIVAVDAAVALIGSTGAVQAGLDDEVAIERANAVDIAADDTRRLEAETTGVSVGVVAA
                     GASVTGSTIGGGATASIGDEVAIGQVAGDTVGELHVLADASHTAVATALAAKGGLGLA
                     ASGTVADAVVDTNVSASIGDDARIKVARTVEVDANSAGAARADATGINVSLGGAVGAS
                     IARATLSPTVSATIGQRSLIDAADFTLAAHQALPAGGGRSAFASASGAAGGLLVGANA
                     SRSTAFNDAAVNARVGDDSTLKISGTATVQTHNTHSQLAYVSGVSAGFVAVGANTASA
                     GSNTDSHAALGDHVRVEGGTLNVLADGQATNHAYGVAGAGGLVSVPFSASYTTTVSRT
                     SATTGSKAARQPGDPATIAVDSLTLAATQGSSFDAWMSNTNASLVGVSGAQTENFARA
                     TTTATIGAEGEIEAGTVQLDASTTLSKGAPAGLLAGTGLARPDWNVESSSGGLADFPA
                     AGSRTILLADTLAGIGAGATVLSTSGNAATPGIRVDAHNEIGASDRVKMASGGAVSAA
                     SGESVVSTVCPPEHAACNAGERPAATVRVGDGAHVETTGESDIAMGSRTSANLHSQSA
                     VDVYGLVGVAPEGSSTAVYDGDNRIEIGDADLRSERNIRLAAGAASDGTANALGASAR
                     TDVFNNTAIPVNRDPVADAFVTSRSAVSVAAGADVAAVRNVALHAVEGSASASGVGIG
                     KDIYRETLAAIASWFSNLFGGGDVSFETRTGRSGVSQDSTVDVRGTVRVGIERRQRLH
                     IDYDGSVIEQTDGIGIASTRMQDIYADILARIETLRALIRDYAVGPGDTTSDAAIATA
                     AYRAEISFLEHKLHEMGFGGNPDEPGFNDSNPRQMAEDRVGALDGQLGGLRTTRTQLQ
                     AEKTTIEGQVATLNQPLDEDNGTRTAQNTTLSNQITGLNAQIAALDPDDDDYATDLAA
                     LRGQVATRQAAIDANNRAITANNQQIALNNAPLDAKNAQIAAVNTEIGSLETTVGKIR
                     QGIAAGDYGDASGAGLQAKFLRIGDSVARLGDIDVRADRLTGTGELDAPGDAGIHIIN
                     EGPSFLTLGKLEIASDAGGRLVFNGVEVRDNAGIAAINRGAGTAAFARIVTAEAGSDA
                     PAILVENRFNPLDDADATRAIGAGVAPVAPDIVLTDAITNLRGLVGIHSEAGGIRLEQ
                     TASIAANSVEVQALNGDFVQSYTDSFTHVATSPLKEVVGSGGTRSIVQDTSGSGSIVA
                     NGSVLVAARYLNINGTIQSGLPEWGVHVPAQAQVELPDGSSVDLATAKAWYEALDPSA
                     RAQPGAEFFKLSNASVSGLTSAQQGQWTQVTVNYNAREDRLELAGVQVQGGYIELFGQ
                     IFNTKAGGGKLRVLDGYGQIEVRNDSGKTLRVNTLDAGRGAEGRIVITDLSVDASGDT
                     VPGTPRTFTRAAGEARSGVDAYTPTTNLRYVMATGFNSVRTDEYLYSQNGWFGITAAP
                     VQDQYRVGTTANTLDPLLQGEFLRVQSLTNPDAFVPTTTTVNGFRSVTQGASWKKCNW
                     WTLCANATYYMQYFVTTGNKTTVTGSVRADHPIPIEYIGFDTARIAIDSVGDVVLAGS
                     LNNRSGTTSVDTDGHLSQGNATATVGGRTIELSAGTGIGSTTPAGEPQQALRIRQLEG
                     GVVHADTTSGSIHLDQVVGNLNVGRIGSAGVDRVTLESAGDILAKDADALVQARRIDL
                     VAENGRIGTLDEAGNGTPLRIRSGDSTSLDPDVLARHGLSARARGNIHLANEADPDPA
                     AAGTYTGDLLLIGVESGAGDVRIRTSGSAIDNNPYGSVDTRTEDQLAALWDELRLRVK
                     ADPDPNDGVAVGADAKAQEAVAAFANGRSNNYRLYWQLRGRQADGGAAYDPGYQYVPT
                     ATERRVLADSGMSDAEIAAFAANRSAQYHALHAEVGGYTTAFDEDFRYVATDAERAAI
                     TRGSSWTEAQLLLSVGAGLIKKVTDTVTTIQEPNVVGRKVTLIADGDIGAYGPDLVID
                     TTAGIDTLSKAERAALAAAERGDAQVDDPDNPRIVTLRQPQAVNLAVAPDGALSARAG
                     GHVLIGAEQDLRIDRIVAGGETRIKVAGGLSGLASATDAVNVKSGGRLILEAASAGIG
                     GTLDPDGLFLTPFGIQLTSPDAGLILRAGGHAWVAAESGLFVDTVFALGDVRLDASGA
                     ILDFQLTEAPLAPELNLLARNVALASRTGAIGSSVNPFDVAVSHEDGRISASTARFGE
                     GAHLNGPMGEFFNIANVTSGDAVALSSATFMFIDGRVTGPGPISLVSGASMVLTPFAD
                     IHATTLGVFLQASDLLMQESADGADAARIQVDVGTIDLRTVGHQAAPDAPRSGGDAVI
                     TGIFTGNPTESAILIDAFGSVLDGGDKRLDIIARTAPAATLTIRAGGQIGGNPLEIDV
                     LHFNADSGGLIHLDSPGSLAMGDVRAEREIDIRAAGSISADSVTSVTEDVRLAADGGD
                     LRVGAIAGRDVELGVQGGNAEIGSIDARDLGLRVENGDARIGSIDARDVDLGARDRLD
                     IDRLDAGRSLRLGGNDIVARVFASGGAPIVGSIGGYGGGMASVADLTLSGPGGFHFDD
                     IAARTGRLDLPLGTLQVDRFQVGERMVVTNPQTRLLIDQQNFRLQGYDVQLYSGGAPF
                     ALALDRNLIATDSFVIDRRASHEVRGPDGPLASVVEYTERVLADIHLAPPAAGAPRED
                     GEESLVRIAPTPVALGTEGE"
     gene            26919..28568
                     /locus_tag="PKN25_05200"
     CDS             26919..28568
                     /locus_tag="PKN25_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNV90330.1"
                     /translation="MLILAAALSAAPALAQTLPSAANPGALQQRRIDEEERRRQLEQL
                     ERREITDPVRREAAPPPAAPPAGEAVRFTVREIRFTESAILPAEALAELAGPYTGREV
                     SLAELRELAARIDAAYRERRVVTARAVIPPQDVSDGVVDIRLVEGRVGEIAVEGNAST
                     DAEFITRRIGLVPGELVDLPVLEQDLRRFNRTQDAQLRADLAAGQRFGTSDLRILVEE
                     PPRHSLRLSLDNGGSEGSGEWRTGLQYTNRSLLGFRDELSLGVVGAKGQESYSAGYSF
                     PVNRSGGRLSLAAYKDHVEIVEGPLRDLDISGRSTGTIVALRQPVHLGERSRTDLVLG
                     SKRRETRNWISSVPLQETQTVDGHLGVEHQAADDHGYWLATYTLTGGRAKGFERSNYT
                     LGRAFVRRVHALAPGWSAQAGLNYQHSGHSPLPSGEQLQIGGEYSVRGYPVGVWSGDR
                     GFIANLELHHPLGELAASEGLPGVRATGFFFADHGRVTPDRAPNSLEPNHRQLGSAGW
                     GVRAALGERVDARLTFARAFNRLPDEDRRYEVHFMLVAEFM"
     gene            28860..>28944
                     /locus_tag="PKN25_05205"
     CDS             28860..>28944
                     /locus_tag="PKN25_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HNV90331.1"
                     /translation="MQLHLIRHPRPAVEPGICYGQTDLGLAE"
BASE COUNT         4538 a        10769 c        10086 g         3551 t
ORIGIN      
        1 gcgacgccag tcgcgatcgg cgacacatcg ggtcgaggcg caatttcgcc acggacccga
       61 tggcagacgg gtcatcctcc gccgaacagc cccgccaccg ccaccggatt cgacgggaac
      121 cagaaatgca cgtacgaggc ggtgatcggg ccgcgacggt agatcgcctc gccctcgccg
      181 ccgtcggggc ggcacgcgcg cagccagggc tccagcgggg tctccgtgcg cgaataatga
      241 aaggtgtggc cggtgagccg accgccgggc agatcgactt cctgcatgcc gagcgcggcg
      301 agccttttct gcatcaccgc ccggccgggc agcaggccgg cgaacccgtg ctccacccca
      361 accttgtcga cgatggcatc gagcaggctc atcaggcctc cgcactcggc gagcaccggc
      421 ttgccggcgg ccacatgcgc tcgcagcccg acccacaacc cggcgttggc ggccagcgcg
      481 gcgccgtgca gctcggggta gccgcccggc agccagacgg cgtcgcactg tgccggcaag
      541 ccctcgccgg cgagcggcga gaagaaggcg agctccgcac ccagcgcgcg cagggtgtcg
      601 acattggccg ggtagaggaa gccgtaagcg gcgtcgtgcg cgatcgcgat ccgcttgccg
      661 gcgagcagtg gcggaatctc cgccactgcc acgacgggaa acgccaccgt cggcggcagc
      721 tccgcggcgc cggtgcgctc gagatgatcg gcgaggcggt cgagacgcgc accgaggtcg
      781 tcgatctccg ccgcctgcag cagaccgaga tgacgctcgg gcagcgccgc atccacatcg
      841 cgcatcaccg caccgaacca gcgcatgccc gccggcagcg actcgcgcag catgtcggcg
      901 tggcgcgcgc tgccaacgtg gttggcgagc accccggcaa agggcaaccc gggctggtaa
      961 tgcgccaacc cgtaggccac cgcgccgaag gtctgcgcca tcgcgcgtgc gtcgatcacc
     1021 gccatcaccg ggatgccgaa gcggcgtgcg atgtcggcgc ccgagggcgc gccgtcgtag
     1081 aggcccatca caccttcgac caggatcagg tcggcctcgc gcgccgcggc gtgcagccgc
     1141 ctggcgatgt ccgcctcgcc gcacatcccc aggtcgacgt tatgcaccgg cgcgccgctc
     1201 gccaccgcgt ggatctgcgg gtcgaggaag tccggcccgc acttgaacac ccgcacccgc
     1261 cgtcccagcc gcgtgtgcag gcgcgcgagc gccgcggtga cggtggtctt gccctggccg
     1321 gaggcgggtg cggcgacgaa gagtgccggg cacgaggccg cacccgggtc gaccgtttcc
     1381 ggcatcgcgt ccgccacgct cactcgccac cctttccgtg caccaccgca cagccggcgt
     1441 gcggcagcgg cgaggccttg ggctgctcct gcggctcgaa ccaggccagc ttgggataca
     1501 gccccaccac gctgccgacc acggtcagcg aggggggacg cacgccttcg tccagcacgc
     1561 gctgcgccag ggtggcgagg tcggccacca ccacgcgctg cgcatgggtg gtgccgcgct
     1621 cgatcacccc cgcaggcgtg gtcggcggca ggccgtgggc gacgagctgg gcgcagatgt
     1681 cggccaggcg cgagatgccc atgtagatca ccaccgtctg atgcggacgt gccagcatcg
     1741 gccagtcgag gtcgaccttg ccttccttga ggaagccggt ggcgaacacc accgactgcg
     1801 cgtgctcgcg atgagtgagc gggatccccg cgtaggcgga gacacccgcc gccgcggtca
     1861 cgcccggcac cacctcgacg tggatgccgg cctcgaccag cagctccatc tcctcgccgc
     1921 cacgaccgaa gatgaagggg tcgccgccct tgagccgcac cacgcgcttg ttccgccgcg
     1981 ccagctcgag cagcagctgg ttgatctcgt cctgaggcag cgagtggtcg gccgccttct
     2041 tgccgacgta gatgcgctcg gcctcgaccg gcgccagatc caggatgggc ggcgcgacga
     2101 ggttgtcgta gacgatgacg tccgcgcccg ccaccaggcg cgcgccgcgc agcgtgaaca
     2161 gctccgggtc gcccgggccg gcgcccacca gatagaccca gcccggcagc gggccgggac
     2221 gatgaaccgc gagatcggga actgccattc ttaccactcc atgccgggca tcgccttcac
     2281 ccccgcggcg aaggcgtgct tgaccggcgt catgtcggtg acggtgtcgg cagcctcgac
     2341 cagcgcgggc ggcgccccgc ggccggtgac caccacatgc tgcatcggcg gccgggcgcg
     2401 caggtcggcg agcacccgct ccacgtcgag atactggtac ttgagcgcga tattgagctc
     2461 gtccagcacc acgaggccga cttcgggatt ctgaagcatt tcgcgtgcca gcgcccaggc
     2521 ggcttcggcc gacgcgacgt cgcgggcacg atcctgggtc tcccaggtga agccctcgcc
     2581 catcacgtgc cagcgcacgc cgggctgggt acggaagaag gcctcctcgc cggtgtccga
     2641 gcgccccttg atgaactgca ccacgccgac ctgcatgccg tgcccgagcg cgcgcgccac
     2701 caggccgaag cccgcgctcg acttgccctt gccattgccg gtgttgacca gcagcacgcc
     2761 gcgctcggcc tgcgcggcgg cgatcttttc atcgacgacg tccttcttgc gtgccatgcg
     2821 ggcgttgtgg cgggtgtcgc ggtcgggggt ctgggtcatg tcgggcctgt cgtcctgttt
     2881 tcgttgtgaa gtcggcgcgc gctggcacgc atgccagcgg cgatcgtggt gaagacatcc
     2941 tcggggaagg ctgccgtcag cccggccgca agcccgtcga gctgcgccag caccgcctcg
     3001 acgacatcct ccatgtcggt cccgaggccg cacttgtgcg ccatcgcgtt gaagtgcctg
     3061 cgtgcggcgg cccgcggcta ccattcacgc tccacctcgg cgggcttgcg gtcgccgagg
     3121 gtcagctcga gcccgagcac gccagcgagc gcaagcaggc ggtcgaaggt gaggcgctcg
     3181 gggcgcgtct cgagttcgga gagccggttc tgactgatgc cgagccgggc cgcgacctcg
     3241 gcctgcgaca agccgagcgc cttgcgccgg ccgacgagca gctcggcggc ctgttccgga
     3301 gagtaaagcc tgggcatggt gtcgtatcga tgatcccgat aaatccagga tatcgccttt
     3361 ctcgataaac gcaagaaatc gccaatgccg atatcgagcc cggcacgcgc tccggacacg
     3421 cccgctcagt ccggcaggaa caccaccctc ccctcatgct cgatccggcg cagcgggtgg
     3481 ccgaaggcct ggccgagcag gtcggcgcgc agcacggcgt cgcggtcgcc ggcgatcacg
     3541 ccgccacggc cgtcgagcag gatgatgtgg tcggcgtagc gtgcggcgag gttgatgtcg
     3601 tgcagcacca gcacgctccc gcgcgcggta ccctgctcgc gcagcaggcg ctggaagagc
     3661 tcgagcgcgg cgatctggtg gtgcaggtcg aggtggttga gcggctcgtc gagcaggaac
     3721 aggcgcggcg cctgcgcgag cagggtggcg atgcccacgc gctggcgctc gccgcccgaa
     3781 agcgtctgca cgtcgcgctg ctcgaagccg gccagcccga ccgactccag cgccgcgcgc
     3841 gcgatcgcca cgtcctgcgg cccctcccag ccccaacgcc ccaggtgcgg gtggcggccg
     3901 accagcacgg tctcgagcac gctcgccgcg aaggggtcgg gctgctgctg cgcgagcagg
     3961 ccgcggaacc gcgccgcctc cagcgccggc caggccgcat agggcaggcc gccgatgcgc
     4021 acctcgccca ccgtgggctc gcgcagtccg gccagggtgt gcaacagcgt ggtcttgccc
     4081 gcgccgttgg ggccgagcag caccaggcac tcgcccgccg ccaggctcag gctgaactcg
     4141 cagcacagcc agcgctcgcc cacctcgagc gcgaggcgct cggcctcgag caagggggcg
     4201 tcatccacgg gcaaggcgcg gcgggcggcg ttcatggcgt gccgcgcggg ttgcgcgaga
     4261 gcaggaagag gaagaccggc acgccgatca gcgcggtgag cacgcccacc ggcagctgct
     4321 gcggcgccat cacggtgcgc gccgcggtgt cggccagggt cagcagggtg ccgccggcga
     4381 gcgccgccgc ggggatcagc acgcgctggt cgttgcccag cgccagccgc accaggtgcg
     4441 gcaccaccag gccgacgaag cccaccgagc ccacgagcgt gaccgcggtg gcggtgagca
     4501 gcgaggcgag cacatagagc gcgacgcgca ggtggccgac gcgcacgccc agcgcgcgcg
     4561 cgctgagctc gccacgcgcg agcacgttga ggtcgcgcgc gtagggcatc accgcgacca
     4621 gcgccagcgc cagcaccgcc agcgccggcc acggccgcgc cgccgcgctg gcgtcgccca
     4681 tcatccagaa cagcatcgac atcacccgcg tgtcgggcgc gagcgccagc atcagcgaca
     4741 ccgccgcgcc gcagccggcg gcgacgatga cgccggtgag cagcagccgc gtctgcgtcc
     4801 agctgccgtc gccatgggcg agcccgaaga cgagcagcat cgccgccagc gccccggcga
     4861 aggccgacac atccacccag aacagcgccg ccccgagcat catcgccccc agcgccccca
     4921 ccgccgcccc gcccgagatg cccaggatgt aggggtcggc gagcgggttg cgcagcagca
     4981 cctgcatcag cgcacccgcg gtggcgagca ggccgccgca ggcgaaggcg gcgagggcgc
     5041 gggggaggcg gagttcgcgg atgacggtcg cccccatgcc ctccgcgccg gccagcgcgg
     5101 cgaagacatc ggtcgccgca accggcaggt cgccggcgac cagcgcccac agcaaggcca
     5161 tggccgccgc ggacagcagt accgcagcga tcgccagcgg cgcccgcctg cggcgcatgg
     5221 ccggactcaa gcgcgactcc ccacgcaggg cgacggcggg catcgccggt cagtgcatcg
     5281 cgtagcgcag cgcgacgaag gcactcctgc cctgtgttgc gaaccccttc gcaagctcat
     5341 ggtccttgtc gagtagattg ttggcacgaa cttcgacgct ccagtcgcgc gccacggcgt
     5401 agtgcgcgta ggcatcgacc agaccgtagc cgccgaggcg attggacacc gcgttcggca
     5461 cctgatcata gcgccgcccc ttgccgttcc actccacacc ccaggtccac gcgcccaccg
     5521 cacgctccag cgcgagcgcc ccgccgacgc gcgcgcggcg gccgagctga tcgccggtct
     5581 cgtcatcctc cgcgtcgagc aggtcgacgc tggcgcgcag ccggtagtcg cccagggtcg
     5641 cggacagcgc cgcctcgatc ccctggatgt cggccctgcc cacattggag gcgatgccgg
     5701 tgctccagtc gatgaggttc ttgatgcggt tgttgaaata ggtcaggtcg acaccgaccc
     5761 cgttgcgctc ccaggccacg cccagctcac ggttgagcgc ctcctcgggc tccaggtccg
     5821 gattgccgcc gaagcagccc caggcaccgt agcactccac cgggaagtac agatcgttga
     5881 aggtcggcgc cttgaacgcg gtgccgatgc tggcatgggc gcgccactcg ggcgcaagcc
     5941 gatagccgta ggccgcggcg ccggtggtct tgccaccgaa ctgcgagttg cggtcgtggc
     6001 gcgcattgag ctggacgttg tggcggtcga attcaccacc ccagccgagc aggaaggcgt
     6061 tgatgtggcg acgcgtcttc tcgaagtcgg aggtggtctt cacgcgctgc tgcagaaact
     6121 cgtacgccgc catcagcgag ccgccaccta cggcgacgtc gtgctgccag ctcaactggc
     6181 gctgcgtggt atcgaagcgg gaacgcgtgc tcgcgttcga ctggttgtcg agggcatcca
     6241 ccccgtgacc gaagcgcagg gtgctggccc agtcggcgga atgctgcttg cgcagatacg
     6301 cgccgaaggt ctcggcacgc ttgtcgaggt aggagtcgaa aggctgtgcc gcgtcgtacc
     6361 agttgcgcat gtcggaatac agcaggttgg cgccgagctc gtcattgccg cccaggtcca
     6421 gcaccagcga cgcgcccaga taatcctcgc ggaagccgtc gtcgtcggca tccgcggcgg
     6481 tcgccgaatt cggccgtgcg ttgaagccgt cgctgcgctc gtgaccggcg gtcaggctgt
     6541 agcgcagacg ctcgtttcca cccgcgagcg tggcgctcgc ctcgcgcgcg ccgtccgtgc
     6601 cggccccgac gcgcacggag ggctggaagc cctcgcgccc cttgcgggtg aacacctgga
     6661 tcacgccacc gagcgcatcc gcgccgtaca gcgcacttcc ggggccgcgc aggatctcga
     6721 tacgctcgat gagttcgagc gggatcgtct ccagcaccgg agagccgctg gtggcggagc
     6781 ccatgcgcac cccatcgacc agcaccagcg tgtgtccgga gttcgccccg cggatgaaga
     6841 tgctgccgct cgaaccagga ccgccattgc gtgccatctg cacgcccggc cgcgaggaga
     6901 gcaggcccag caggctcgaa tgccccgcgc gggcgatttc gtcgcgctcg atgacatcca
     6961 cgctggccag ggccgcatcg gcactgatcg gctggcgagt ggcggtcacg acgacgtggc
     7021 cgagctgggc atcggccgca tgagcggccg gtgcgaggaa acaggcgagc gcgcatgccg
     7081 aggcgagcgc ggaaacacgg atcttcattg gtttgaactc ccttcgccac accgtgcgtc
     7141 cccgcacgcc atggcaacct cacatggacg caccgcgagc caggtagaac cgaagagagc
     7201 aggagaaccc accgcgcccg tcgccgccac cccgcggcac atccgccaat ccgcgttccg
     7261 gccggtctcc gggctcgtgc gctgtgccgg cggggccgga cgggtgagtc gccttcccgg
     7321 gcatggatgc ccagtggctg catgacgcac cttgcgcgca cttaccgttg cgggggcagc
     7381 acaggcattg cggcctcgtt tgcgaggggc cgcgcacctg tttcccgttt aacccggggc
     7441 gagaaacgcc cgtgggcacc gataacgcgt tgccccttcc ggggcagtgg gggcggatga
     7501 taccgtacgg cccggtatcg tgcactgcaa aaaaacgacg acgaatcctc catgcaggac
     7561 ccgcaaagac catgacctgc gaactcatcc tcggcggcgc ccgctccggc aagagccgcg
     7621 aagccgaacg gcgtgctgtc gaaaccggcc tggcggtgac ggtgatcgcc accgccgagg
     7681 ccctcgacga ggagatggcg gcgcgcatcc gccgtcacca ggccgaccgg ccggcgggct
     7741 ggcgcacggt cgaggcgccg ctggcgctcg ccgatacatt gcgacgcgag gccgcggccg
     7801 agcgctgcct gatcgtcgac tgcctcacgc tgtggctctc caacctgctc gccgacgccc
     7861 acatccttcc cgcaggcgcc acggccgaaa acctgccgcg cttccgccac gagcgcgacg
     7921 cgctgctcgc cgtgctgccg acgctgcccg gccgcatcgt cctcgtcgcc aacgaggtgg
     7981 ggctcggcct ggtgccggag acgccgctcg gacggctgtt ccgcgacgag gccggacggc
     8041 tcaaccagat ggtggcgagc gcatgccgcc gggttgtctt cgtcgccgcc ggcctgccgc
     8101 tggtgctgaa ggaaggctaa acttcggggc gaagcacact ttccgggaga gggctgtggg
     8161 gccgcctgca cagaagcaca agatgacgct ggaagacttc ctggcctggg aggcggagca
     8221 gcccgagcgc tgggagttct tcaacggcga ggccttcatg atggcggggg ggtcggacgt
     8281 gcataacgtc gtgaccggaa acatgtacat ggctttgcgg atcgggctgc agcacacgcg
     8341 ctgcaacgtc ttcatgtccg acgtccgtct gcgcctggcc gaatcggacg acctcttcta
     8401 ccccgacgtc ttcgtcacct gcaccgacgc cgaccgcgcc cgccgccagg tcaaggaaga
     8461 ccctatcctc atcgccgagg tgctgtcgcc ctcgaccgag gcctatgacc gcggcgacaa
     8521 gttcgccgcc tatcgccgct ttgcggggct gaagaccgtg ctcttcctgt cgcaggaccg
     8581 tgcccacgtc gagtgcttca cccgcggcga ggacgggcgc tgggtgctga gcgaggcgag
     8641 cggagaaacg gcccgcctcg ccctgcccgc cttcgacttc gagctcgcgc tcgccgacct
     8701 ctatcgcgac ctgccggacg cggacgcctc cgacccggcg cccatccccc ctccctccac
     8761 accacccccg ccacctgccg catgaagccc tacgccatcg cctcgcccga cccgctgctc
     8821 tccgccgcga tccagcacaa gatcgaccgc aagaccaagc cgcgcggcgc gctcggccgc
     8881 ctggaggcgc tcgcgcacca gatcgcgctg ctgcagcaga ccctggaacc cgaactgcgc
     8941 cagccgcaca tcgtcgtctt cgccggcgac cacggcgcgg cgcgtgccgg cgtgtccgcc
     9001 tacccgcagg acgtgacctg gcagatggtg gagaacttcc tcgccggcgg cgcggcgatc
     9061 aacgtattca cccgccagct cgggctggat ctcaccgtgg tcgatgccgg cgtcaaccac
     9121 gacttcggca agcgcccggg gctgatcgac gccaagatcg cgcccggcac ggccaactac
     9181 atcgaagagc cggccatgag cgccgcgcag cgcgacgcag cgctcgagcg cgggcgcggc
     9241 gtcgcccatg cgctcgccga caacggctgc aactgcatcg gcctgggcga gatgggtatc
     9301 ggcaacaccg cgtcggcctc gctgctcacc cactgtctgg tgggcgccga gcacgacgcc
     9361 gagctctata ccgtcaccgg ccgcggcacc ggcctggacg accccggcct gttccgcaag
     9421 cgcagcatgc tcgcccaggc gctgcagcgc ggtggtcgac cacacgaccc gctggaggtg
     9481 ctggtgcagt atggcggcct cgagatcgcc atgatggcgg gcgccatcct cggcgccgcc
     9541 gagcgccgca tgctggtgct ggtcgacggc ttcatcgtca cctcggccgt gctcgtcgcc
     9601 cacgccctgg cacccgcggt gctgtcgtgc tgcgtcttcg cgcatcgctc gcaggagccc
     9661 ggccaccgcg tgcagctcga cttcctgcag gccgaaccgc tgatggaact cgacctccgc
     9721 ctgggcgaag gcaccggcgc ggcactcgcc ttcccgctcg tgcaggcggc ggtgaatttc
     9781 atcaacgaga tggcgagctt cgagtccgcc ggggtcagcg accaggcccg tcgtccgggc
     9841 tgatgcgcga ccagctcgaa ctcttcttca ccgcgctcgg cttcttcacc cgcctcccgg
     9901 tgcccgcctg ggtgccgtgg tcgtccgagc gcatgcacca tgcggcgcgc tacttcgcac
     9961 tcgtgggctg ggtggtgggc ggcgcaggcg cgctcggcta cctcgccctc gcctggctgc
    10021 tgccgcccgc cgtggcggtc gtcctgtcga tggcgctcac catccgcctc accggcgcct
    10081 tccacgagga cggcttcgcc gacagctgcg acggcctcgg cggcggctgg gacaaggcac
    10141 agatcctggc gatcatgaag gactcgcgga tcggcagcta cggcaccatc ggcatggtgc
    10201 tgatgctgct cgccaaggcg gcggcgctgg tggagctggc cagcccggcg gtgcaagggg
    10261 cgggcgtggc gctcgccctg ctcgtcgccc atccgctgtc gcgcctggcc gccaccgtgg
    10321 tgctgcaccg cctgccctac gtacgcgccg acgattcggc caagtcggtc gccgtcgcgc
    10381 gcccgctggc accggccggg ctcgcgctgg ccgtcgccag cggcctgcta cccctgctgc
    10441 tgctgccacc cgccgccgcg ctctgcgccc tgctcgccgc cgctgcggtg acgacgtgga
    10501 gcgcgcgcct cttccagcgc cggctcggcg gctacaccgg cgacctgctc ggcgccaccc
    10561 agcaggcgag cgagctcgcc atctacctcg ccctgctcgc cgcatcgcgg ctggcgagct
    10621 gagcctgcac cgccgtgcaa tccccatgcg gcctgggcgg atcttgaccc aggacaaaaa
    10681 aaacttgctc ccgacaagtc ggcaacccaa tatgagttga tctggagtct ttctccggca
    10741 agtcggacac gtcttcaggc gtgcgcggaa agcatggtgg agccccgacc tgaagcgcgg
    10801 ctccgcgccc tcgacaagag gggcaggggg agacaagcca tgcatcacca tcgcgagccc
    10861 gagccggtcg cagctgcgcc gcgcctgaag gaaatcgtcg tccatgtcac ttgcgcgctg
    10921 atcgtcagcc acggcctggt gcccgagctg gcgtgggcgg tcgacatcgt ccccgacggc
    10981 cgcaccgcga ccagcgtgag cccgctcggc acgaacgccg ccgacgtccg caacaccgac
    11041 cgcctcctcg gcaacggcaa tgcggtcaac tccttcagcc gcttcaatgt cggcagcggc
    11101 gagaccgcca acctccacct gcccgacggc gccgccgcgc tcatcaatct ggtgcgcgac
    11161 cagaagacca cgatcgacgg catgctcaat gccatcaagg aaggccggat cggcggcaag
    11221 gtctatttcg ccaacccgca cggcgtcgtc gtcggcccga gcggcaaggt caacgtcggc
    11281 agcctgcacg tgtccacgcc cagccagcgc ttcgtcgacg acttcttcga cgccccgggc
    11341 ggcgccgagg cctcggccgc cgccttggtc gagggccgcg cgccgcgcaa caccgcggcc
    11401 gagatccgca tcgacggcga ggtcaacgcc accgagcagg tcagcctgtc cgcaggctcg
    11461 atcacggtgg gcggcaaggt gctgagcggc gcgaagttcg ccggcggtgc ccccgacttc
    11521 agcgacgtgg tcaacgtgca cggcctggcg acgggcggcg cggtggtcga gaaccagggc
    11581 cgcatcgaaa tcgtcggcga cagcggcatc gagctcaccg gcacggtctc ctcgcgccgg
    11641 gtgaacgtcg gcgacgacgc cagcctgcgc agcggcgtgt cgatcgccgc ctcgggcgac
    11701 atcgccctgc gtgcgcccac gatccgcatc ggcagcggcg gcaagctgct cgccgcgggc
    11761 agcgacaccc acgcgggcgg agacgtgacg ctgcaggcgc aggccgtcgg cggcacgctc
    11821 gacgccttgt ccgacaccgc ggcgcgcatc gaggcgagcg gcgcgaccct caccggccgc
    11881 gacctgcacc tgcaggcgag cgcggaacac cgtagcacgc tggcgccgct ggtcaccaag
    11941 accacgagcg cgacgatcga ggtggacgga tccacgctgg aggcgagcgg ccgcgccacc
    12001 ctcgaggcca ccgccaccgc cgccatcgcc accccggatg tcgtgccgct ggcggtggtc
    12061 gatgtggacg tcgcggcgag cgtgcgcgtc cagggcgaca cccggatcac cagcgccggt
    12121 cccgcccgcc tcggcgccac caccaccacg accatcgacg cccgtcccgg cctgcccgac
    12181 ctcggcgcgc tgccggccga cgccggcgcg gcgatcgtca tcgccgacac caccgccagc
    12241 gtggatctgc tcggcaacgc cgagctcgcc agcacggcgg acaagaccga gctcgtcgcg
    12301 agcaaccgca cgacggtgga cgcggcggtc gacgcctcgg ccgcgggcag cagcgcggtg
    12361 ggcggcagcg tggcggtcag cgtggtgcgc agcaccaccc gcgcgcgcac cgccgacaac
    12421 gcgcgcctgg ccggcaagac cggtgtcggc gtgcacgcgc agtcggatac ccgcgtcacc
    12481 acctcggcca aggccgcggc cggcggcgcc agcccgcagc cggcaggcac gagcaagacc
    12541 gaggagacgc tcgcggacta cgaggacgag gcctcgacct cggacggcag tgtcagcgtt
    12601 gccgcggcgg tggcggtgag cgacctcgag agcaccaccc tggccgcgct cgacgccacc
    12661 cgggcggcca ccagcgaggg catgctcgcg gtcggcagcg aggcactgac ccaggcggac
    12721 gtggtcgccg acggcagcag caccggtgct gccgtcggcg tcggcgcggc ggtcgcgctc
    12781 aacctcgcgc gcacgagcgc ggaggcgcgc atcggcggca gcgccgacgc acagggcatc
    12841 gaggtcgtgg caaccctgcc cacgggcgac gagatcaatg gcttcaccac cgccgccacc
    12901 tcgggcgcgg gtgccagcga cgtcggcgtg gccggcgcgc tcgcgctcaa cgtcgtcgac
    12961 gcgggcagcc gcgcccgcat caccggcagc gccgacgcga agggcggcac cgtccgcatc
    13021 ggcagcgacc accgcagcga atcctcggcc agcgcgaccc ccgccgagga cgccaccgcc
    13081 agcggcgaca ccctcggcat cggcgcctcc gtggcggtca acgtcgtcgc caacgagagc
    13141 cgcgccgagc tcgccaacgg cgcgcgcctg atcaacccgg gcgccgtcac ggtcgaggcc
    13201 gtcggcgact acctcgtcca caccgaggcc gaggctggcg ccgagggcgg catcgccgcc
    13261 accccggtgg ccgcggtcgc ggtggtcgac aaccagaccc atgccgtgat cggcagcggc
    13321 gaacgcgtgc aggccggcgc caaggtcggc gtcaccgcca cccattccag caccgtcgaa
    13381 acctcggcca agggcagcgc cgagggcgag aaggccgcga tcggcatcgc cgtggcggtc
    13441 aatgtgctca ccgaacgcgt cagcgcaagc atccagcgcg acgtgttcac cccgggcgcg
    13501 atcgaagtgg ccgcgcacaa cgcccacaag ggctcggcga ctgcgagcgc gagcgccaag
    13561 ggcggcaagg gcgaggaaga ggacggcagc agcgccgagg acggcgtgga cctcgaggtc
    13621 cagcgccagt ccggcttcgc ccaggacaag cagaaggacg gcagcgcgag caagacccag
    13681 cagccgggct cggccgaatc gagcgagggc aagctcagcg tggccgcggc ggtcgcggtg
    13741 aacgcgctcg acagccgcag cgaggcctac gtcgccagcg ccatcgagtt gcaggccggc
    13801 ggccggctcg cggtcgaggc ctggagcaac accgacgccg aagccgaggc cgatggcagc
    13861 gcggccggga gcacggcgac ggtcggcatc ggcgccgccg ccgcggtcaa cagcatcgag
    13921 acccacaccc tggcctggat cggcgacgct caggtgaccg cgaacggcat cgccgtaaac
    13981 gccggcacgg aaatcctcgg cgacgacgag gagaaccgct tcagcgcgag cgcgaaggct
    14041 ggcgccggcg cgggcaaggt cggcatcgcc ggcgcgctcg cgctcaacct gctcgagacc
    14101 cacacccgcg ccgaggtgct cgaaggcgcg cgcgcggacg ccgagggtgg cgatgtcgag
    14161 ctcgaagccg acagcaccac ggaaaccagc gccaccgccc tgcccgacga gccggtcgac
    14221 ggcggcgact tcggcgtcgg tgcctcggtc gcgctcaacc tcttccgcga ggacgaggtg
    14281 cgggcacgca tccggcgcgg cgccgagatc accgacctcg acaagctgcg tgtgatcgcc
    14341 aacgccagca gcgacacccg cgccaccgcc gaggcaggcg ctgcgggcag cgtggcgctc
    14401 gacgccgccg tggccctgtc cgacctcaag ctgaacacgg ccgccgtggt cgaggccggc
    14461 accgaaatcg tcgccgccgg cgccgtcgag atccgcgcca cctccaccgg cgagcacgag
    14521 gccgaggcga cgggcgacac cgaatcggaa aaggtcggca tcggcgcctc gggcgcgatc
    14581 gtgctcagca cgaccacgac caccgccagc ctgcagcgct cgctgcagac gaccaccagc
    14641 ggcgaggagg gcaatctcac catcgacgcc gacgccacgc gcagctacga ggcggtggcc
    14701 aaggccagcg ccgccggcgg caaggccgag gaggacacca ccgccgccga gcgcgacaag
    14761 gccagcacca gctcgaccct gaaggacaac caggacgccc aggagggcac ccagaccacc
    14821 ggcaacagca acaaactggc ggtggcggcc gcggtgggcg cggtggtcat ggacgacgac
    14881 gtgcgcgcgg acgtcgggcg tgcgcagggg gcggacagcg accctgccgt ccgcctcgac
    14941 gtcggcggtg aactcgcgat cggcgcacac aaccagagcg acttcagcgc ccgcggcctg
    15001 ggcaacaccc tggacgtgtc caagctcgag agcagcgccc aggtcggcat cggtgtcggt
    15061 gtcgggttgg cgatcgcgcg caacgacacc gccgccacga tcggcgacgg cacgctggtc
    15121 gcggacgcgg cgggcgtgca agttgccgcc ggatcgtcgc agaacacctc ggcggagttc
    15181 gccaacaagc tcgccgcaga aggcgtggcg ggcgccgggg cggagaaggt cggcatcgcg
    15241 ggcgcacttg cggtggcatg gtcgcaatcc accacctccg cctcgctggg cgagggcgtc
    15301 cgcatcggcg atcggaccac cggggagtac gccggcgaag tcagcatcac cgcggacaac
    15361 accagcaagc tcgcggccaa ggcctggagc gcggcgctcg ccggcaaggt cggcgtcggc
    15421 gcctcgatcg ccatcctgcg cgccaacaac agccagcatg cctggatcgg cgaacaggcc
    15481 gaggtcaccg cggccgggct gcagctcgcc gggcgcaacc atctgctgaa cagctcgccg
    15541 agcttcgact ggacgatcct cgacgacctc gagaaccgct tcaccgaggc caacctgcag
    15601 gtgctgctcg gcgaaagcaa ctactacacc gagaccatcg ccggcgccgg cggcgagcag
    15661 gtcgcggtca ccggctcgtt ctcggtgaat gtcttcgacg aacgcaccga ggccacgatc
    15721 ggcgacggcg cgcgggtgaa caccagcggc acggtcgacc tggaagccga caaccagtcc
    15781 tccgcgaccg ccttcgccgg cggcgtgtcg gccgcgggca aggtcggcgt gggcctggcc
    15841 agcgccgaca tcgtcaaccg cagccagacc cgggccacga tcggcgcgga cgcgcgcatc
    15901 gagcagtccg ccgcgatcga catcgacgcc acggccgcga tggatctcgc cgcggtcacc
    15961 gcgagcgcgg ccgccgcggg caaggccggc gtgggcggcg tgctgagcct gatcgactcg
    16021 gagaaccagg tcgaggccgc caccggcgcg cgcagcttcc tgcgcagcga cggcgccatc
    16081 gacgtcgacg cgggcaacac cttcgatgcc ctcggcgtcg ccggggtggc cggcgtgggc
    16141 ggaaccgccg gggtgggcgt atccgccggc accttcctgg tcgacaacca gactcgcgcc
    16201 tcgatcggcg ccgcggccga cgtcgccgcg gcgggcgcga tcggcgtgcg cggcaccgcg
    16261 gtgcaggatg tcacctcggt cgtcgtcggc ggcgccggag gcggcaccgc gggcgtagcc
    16321 gcatccgcgg cagtgaacga ctacaacccg ctcacctacg cgtggatcgg cgacggcgca
    16381 cgcatcaatc agggcgtgag cggcgcggcc cttgcgggcc gcagcctggc ggtgcacgcg
    16441 cacgcccgca ccgacttgct gtccatcgtc ggcacggtag gcatcggggg caccgccggc
    16501 gtcggcggcg ccgccgacgt ggtggtgatc gacaaggaga ccgcggcctg gatcggcgag
    16561 ggcgtgatcg cccgcagcgg cgacagcctc gcggtgggcg ccagcagcgc agaggcgatc
    16621 cgctcggtgg gcgtgggcct gagcgcgggc ggcacggccg gtgtgcaggg ctcggcttcg
    16681 gtgctggtgc tcgacatcga cacgctcgcc tcggtgaaga cgggcagcgc gctgttctcc
    16741 gacggcaacc tggtgatcgc ggccgacgcc gacagcgacc tcgacctgct cgccggcgcg
    16801 atcggcgcgg cggggaccgc ggcggtgggc gccggcgcgg cggtcgcggt ggtggacaag
    16861 accacccacg cctggatcga cgacggcgcc gcggcgagcg cgctcggcaa cgccgccgcg
    16921 gcacaggtgg cggacggcgg cttcgacatc gactacgtgg ccacggctgc gggtgaaggc
    16981 gaggtcgccg cccccggcat caccccctcc gacggcgaga acgacctcgc cggcggcagc
    17041 gcggcgctcg acgccacacg caccagcagc gcgagcacga gcgcgcaccg cgggctcgcg
    17101 gtgaccgcgg tgaaccgcga cgacatcaag ggctttgcgg tcaccggcgc cgccgccggc
    17161 accgcggcgg tgaccctgtc gggcgaagtg tcggtgcacc agaccgacac ccgcgcctcg
    17221 atcggcagca tcgcgctcgc cgagggcgcc agccacaccg gcgtgcaggt caacgccgac
    17281 aacaccggcg ccaacgccgc gcaatcggtg cgcgtcgccg ccggcaacga cagtttccac
    17341 ctcggcatcg ccggcgcgct gtcggcctcg ggcacggtgg gcgtaggcgt cggcgccgat
    17401 gtggcggcga tgcggcacgc cacccatgcg gccatcgtcg agcagagcga cgtcaaggcg
    17461 cgccgcgacg tcgaggtcgc cgccagttcc ggtcaggagg tggtctcgat cagcgccagc
    17521 ctgggcgcct cgggcacggt cggggtggcg ggcagcgtgt cggtgctgag cttcgacaac
    17581 gcgacctcgg cctcgatcgg cgacgaagcc gaagtcgccg ccggcggcaa cgtgagcgtg
    17641 aaggcggacg accacaccgc cacgaccctg gtcgccggca cgatcgcgct cggcatcggc
    17701 ggtggcggag tcggcgccgg cgtgggcgtg acgctgatcg agaaggacac gcgcgcctgg
    17761 atcggcgcgg gcgcggtcgt ggatgccctc ggcaaggaca ccgccgatct ggtcgtgcgc
    17821 tcgggcgaga gcgtggacga gaccgccacg gcgcgcggcg tgcaggtgca ggcggagtcg
    17881 agcgaggatc tgttcaccgt ctcggccgcc gccgcgggcg ggctctacct cggcttcgcc
    17941 ggcgcggtgt cggtggcgac ggtggattcg gacaccgcgg cctggatcgg cgcggacgcc
    18001 cgggtgaacc ggggcggtgg cgacgccgac gcggcgcagg acgtcaacgt cggtgcgcgc
    18061 aacgagttgg cgctcttcaa cgtctccggc gcgctcggcg tcggcgcggc cggcatcgcc
    18121 ggcggcgtgg atgtgggagt gatccgcaac gacaccaccg cctggatcgg cgaccgcgcc
    18181 gaagtgggcg ccaagcgcga catcggcgtg agcgcgctcg cgaacaccga catcgagagc
    18241 tacgtggtga gcgccgccgg ggggatcggc gcgctcgccg gcggcgtcgg cgtgtattcc
    18301 gtgggcggtg ggctgggcga cgaggcgcag ggccggctcg agtccggcga cgacgatgcc
    18361 ggcagctatg tcgatggcca ggccaccgac ggcagcgtcg ccaccctgct cgggagctac
    18421 acggacagcc gcatcggcga ggccgccgat cgggtggagg acgagcgtgg cacgctcgcc
    18481 gcgagcggcc gcttcaccgc ggcggaaacc cgcaccctgc ccgccggtac cgccgccttc
    18541 gtgggcgaga atgcggacct cgaggcgggc agggacatcg acctcgatgc acgcacggac
    18601 gtcgagttcg atgcgctcga cggcgccctc gccatcggcg cgatcggcct cggcgccggc
    18661 gtgggggtcg cggatttcga caacgacacc ctcgcctccg tcggccgggg cagcaccctg
    18721 cgtgccggcg ccgccggcag cgtgagcctg accgcggcgc tcgacgagga tgtggacgtg
    18781 accgccttcg ccggcaccgg cggcatcgtc gcggtcgacg cggcggtcgc gctgatcggc
    18841 tccaccggcg cggtgcaggc cggcctcgac gacgaggtcg cgatcgagcg tgccaatgcg
    18901 gtcgacatcg cggccgacga cacccgcaga ctggaggccg agaccaccgg cgtcagcgtc
    18961 ggcgtggtcg ccgcgggggc ctcggtgacc ggcagcacga tcggtggcgg cgccaccgcc
    19021 agcatcggcg acgaggtggc catcggccag gtcgcaggcg acacggtcgg cgagctgcat
    19081 gtgctcgccg acgccagcca caccgccgtc gccaccgcgc tcgccgccaa gggcgggctc
    19141 gggctggccg ccagcggcac ggtggccgac gcggtggtcg acacgaacgt ctccgcaagc
    19201 atcggcgacg acgcccggat caaggtcgcc cgcacggtcg aggtcgacgc gaacagcgcc
    19261 ggcgcggcac gcgccgacgc caccggcatc aacgtgagcc tgggcggggc ggtcggcgcc
    19321 tcgatcgcgc gcgccaccct gtcacccacg gtgtccgcca cgatcggtca acgcagcctc
    19381 atcgacgccg ccgacttcac actggccgcg caccaggccc tgcccgccgg cggcggacgc
    19441 agcgccttcg ccagcgccag cggcgccgcc ggcggcctgc tggtgggggc gaacgcctcg
    19501 cgctccaccg ccttcaacga cgccgcggtg aacgccaggg tgggcgacga cagcacgctg
    19561 aagatcagcg gcaccgccac cgtgcagacg cacaacacgc actcccagct cgcctacgtg
    19621 tccggcgtgt cggcgggctt cgtggcggtc ggcgccaaca ccgccagcgc gggctcgaac
    19681 accgacagcc acgccgcgct cggcgaccat gtccgggtcg agggcggcac gctgaacgtg
    19741 ctcgccgacg gccaggccac caaccatgcc tacggtgtgg ccggtgccgg cgggctggtc
    19801 tcggtgccgt tctcggcgag ctacaccacg accgtgagca ggacgagcgc cacgaccggc
    19861 agcaaggccg cgcgtcagcc cggcgacccg gcgacgatcg cggtcgacag cctgacgctc
    19921 gcggcgacgc agggctccag cttcgacgcc tggatgagca acaccaacgc gtcgctggtc
    19981 ggcgtgagcg gcgcgcagac cgagaacttc gcgcgcgcca ccaccaccgc gacgatcggc
    20041 gccgagggcg agatcgaagc cgggacggtg cagctcgacg ccagcacgac gctgagcaag
    20101 ggggcgcctg ccggcctgct cgccggcacg ggcctggcac gcccggactg gaacgtggag
    20161 tcgagctcgg gcgggctcgc cgactttccc gccgcaggca gccgcacgat cctgctcgcg
    20221 gacacgctcg ccgggatcgg cgcgggcgcg accgtgctct ccacgagcgg caatgccgcc
    20281 acgccgggga tccgcgtcga tgcgcacaac gagatcggtg ccagcgaccg ggtcaagatg
    20341 gcctcgggcg gcgcggtgtc ggcagcgagc ggcgagtcgg tcgtgtcgac cgtgtgcccg
    20401 ccggagcacg ccgcctgcaa cgcgggcgaa cgcccggccg cgaccgtgcg cgtcggcgac
    20461 ggcgcccacg tcgagaccac gggcgagagc gacatcgcga tgggctcgcg gaccagcgca
    20521 aatctccatt cgcagagcgc ggtggacgtc tacggcctgg tcggcgtcgc ccccgagggc
    20581 agcagcaccg cggtgtacga cggcgataac cgcatcgaga tcggcgacgc cgatctgcgt
    20641 tcggaacgca acatccgcct ggcggccggc gccgcgagcg acggcaccgc gaacgcgctc
    20701 ggcgcgagcg cgcgcaccga cgtcttcaac aacaccgcga tcccggttaa ccgcgacccg
    20761 gtggccgacg ccttcgtcac cagccgcagc gcggtgagcg tcgctgccgg cgccgacgtg
    20821 gccgcggtgc gcaacgtggc cctgcacgcc gtggaaggct cggcgagcgc ctcgggcgtg
    20881 ggcatcggca aggacatcta ccgcgagacc ctggcggcga tcgcgagctg gttcagcaac
    20941 ctcttcggcg gcggcgacgt ctccttcgag acccgcaccg gacgctccgg cgtaagccag
    21001 gacagcacgg tcgacgtccg cggcaccgtg cgcgtgggca tcgagcgcag gcagcgcctg
    21061 cacatcgact acgacggttc ggtgatcgag cagaccgacg gcatcggcat cgcatcgaca
    21121 cggatgcagg acatctacgc ggacatcctg gcgcgcatcg agaccctgcg ggcgctgatc
    21181 cgcgactatg cggtaggccc gggcgacacc accagcgatg cggcgatcgc caccgcggcc
    21241 taccgggcgg agatctcctt cctggagcac aaactccacg agatgggctt cggcggcaac
    21301 ccggacgagc ccggcttcaa cgacagcaat ccgcgccaga tggccgagga ccgcgtgggc
    21361 gcgctcgatg gacagctggg cggcctgcgc accacgcgca cgcaattgca ggccgagaag
    21421 accacgatcg agggccaggt cgccacgctc aaccagccac tcgacgagga caacggcacg
    21481 cggacggcgc aaaacacgac gctgagcaac cagatcacgg gattgaacgc gcagatcgcg
    21541 gcgctggatc cggacgacga cgactacgcg accgatctcg ccgccctgcg gggccaggtc
    21601 gccacccggc aggcggccat cgacgccaac aaccgcgcga tcaccgccaa caaccagcag
    21661 atcgcgctca acaacgcgcc gctcgacgcc aagaatgccc agatcgccgc ggtgaacacc
    21721 gagatcggca gcctcgagac cacggtcggg aagatccgcc agggcatcgc cgccggcgac
    21781 tacggcgacg ccagcggcgc cggcctgcag gcgaagttcc tgcgcatcgg cgacagcgtg
    21841 gcccggctgg gcgacatcga cgtgcgcgcc gaccgcctca ccggcaccgg cgagctcgac
    21901 gcgccgggcg acgccggcat ccacatcatc aacgaggggc cgtccttcct cactctgggc
    21961 aagctcgaga tcgcctcgga cgcgggtggc cggctggtct tcaacggcgt ggaggtgcgc
    22021 gacaacgccg gcatcgccgc gatcaaccgc ggcgcgggca cggccgcgtt cgcgcggatc
    22081 gtcaccgccg aggcgggcag cgacgccccc gccatcctgg tcgagaaccg cttcaatccg
    22141 ctcgacgacg ccgacgccac gcgcgcgatc ggcgccggcg tggcgccggt ggcccccgac
    22201 atcgtgctca ccgacgcgat caccaacctg cgcggcctgg tcggcatcca cagcgaggcc
    22261 ggcggcatcc ggctcgagca gaccgcgtcg atcgcggcca acagcgtcga agtgcaggcc
    22321 ctcaacggcg acttcgtgca gagctacacc gacagcttca cccacgtggc cacctcgccg
    22381 ctcaaggagg tggtcggctc gggggggacc cgctccatcg tgcaggacac cagcggcagc
    22441 ggcagcatcg tcgccaacgg cagcgtgctg gtcgcggcgc gctacctcaa catcaacggc
    22501 accatccaga gcggcctccc cgagtggggc gtgcacgtcc ccgcgcaggc ccaggtggag
    22561 ctgcccgacg gcagcagcgt cgacctcgcc acggcgaagg cctggtacga agcgctcgac
    22621 ccgagcgcgc gcgcccagcc cggcgccgag ttcttcaagc tctccaacgc ctcggtgagc
    22681 ggcctcacgt ccgcgcagca ggggcaatgg acgcaggtga cggtcaacta caatgcgcgc
    22741 gaggaccgcc tggagctcgc cggcgtgcag gtgcagggcg gctacatcga gctgttcggg
    22801 cagatcttca acaccaaggc cggcggcggc aagctgcgcg tgctcgacgg ctacggccag
    22861 atcgaggtgc gtaacgacag cggcaagacc ctgcgggtga acacgctcga cgccgggcgc
    22921 ggcgcggaag gcaggatcgt catcaccgac ctgtcggtgg acgccagtgg cgacacggtg
    22981 ccgggcacgc cgcgcacctt cacgcgcgcg gcgggcgagg cgcgcagcgg cgtcgatgcc
    23041 tacacgccga cgaccaacct gcgctacgtg atggccaccg gcttcaactc cgtgcgcacc
    23101 gacgagtacc tgtactcgca gaacggctgg ttcggcatca ccgcggcccc ggtgcaggac
    23161 cagtacaggg tcggcaccac cgcgaacacc ctcgatccgc tgctgcaggg cgagttcctg
    23221 cgcgtgcaga gcctgaccaa cccggacgcg ttcgtgccca cgacgaccac ggtcaacggc
    23281 ttccggtccg tcacccaggg ggcgagctgg aagaagtgca actggtggac cttgtgcgcc
    23341 aatgcgacgt actacatgca gtacttcgtc accaccggca acaagaccac ggtcaccggc
    23401 agcgtacgcg ccgaccaccc gatcccgatc gagtacatcg gcttcgacac cgcccgcatc
    23461 gccatcgact ccgtcggcga cgtggtgctc gccggcagcc tcaacaaccg cagcggcacg
    23521 acctcggtcg acaccgacgg ccacctgtcg cagggcaacg cgaccgccac cgtcggcggg
    23581 cgcacgatcg agctgtcggc cggcaccggc atcggcagca cgacgcccgc gggcgagccg
    23641 cagcaggccc tgcgcattcg ccagctcgaa ggcggcgtgg tccatgccga caccaccagc
    23701 ggctcgatcc acctcgacca ggtggtgggc aacctgaacg tgggccgcat cggcagcgcc
    23761 ggggtggaca gggtgaccct ggagtccgcc ggcgacatcc tcgccaagga tgccgacgcc
    23821 ctggtgcagg cgcggcgcat cgacctggtc gccgagaacg gccgcatcgg cacgctggat
    23881 gaggccggca acggcacgcc gctgcgcatc cgcagcggcg acagcaccag cctcgacccc
    23941 gacgtgctcg cccgccacgg cctgtcggcg cgcgcgcgcg gcaacatcca cctcgccaac
    24001 gaggccgacc ccgacccggc ggcagcaggc acctataccg gcgatctgct gctgatcggc
    24061 gtggagtcgg gcgcgggcga cgtgcgcatc cgcaccagcg gcagcgcgat cgacaacaac
    24121 ccctacggct cggtcgacac gcgcacggag gaccagctcg ccgcgctgtg ggacgagctg
    24181 cgcctgcgcg tgaaggccga ccccgacccg aacgacggcg tcgcggtggg cgccgatgcc
    24241 aaggcacagg aggcggtcgc cgccttcgcc aacggtcgca gcaacaacta ccgcctgtac
    24301 tggcagctcc gcggccgcca ggccgacggc ggcgcggcct acgacccggg ataccagtac
    24361 gtgcccaccg ccaccgagcg ccgggtgctc gccgattcgg gcatgagcga cgccgagatc
    24421 gccgccttcg ccgccaaccg cagcgcgcag taccacgcgc tgcacgccga ggtcggcggc
    24481 tacaccaccg ccttcgacga ggatttccgc tacgtcgcca ccgacgccga gcgtgccgcg
    24541 atcacccgcg gctcgagctg gaccgaggcg cagctgctgc tgtcggtcgg cgcaggcctg
    24601 atcaagaagg tcaccgacac cgtcaccacg atccaggaac ccaacgtcgt cggccgcaag
    24661 gtcacgctca tcgccgacgg cgacatcggc gcctatggtc cggacctggt gatcgacacc
    24721 accgccggca tcgacaccct gagcaaggcc gagcgcgccg cgctggccgc ggccgaacgc
    24781 ggcgacgccc aggtcgacga cccggacaac ccgcgcatcg tcaccctgcg ccagccgcag
    24841 gcggtgaacc tcgcggtcgc gcccgacggc gcgctgagcg cgcgggcggg cggccatgtc
    24901 ctgatcggcg ccgagcagga cctgcgcatc gaccgcatcg tcgccggcgg ggagacgcgg
    24961 atcaaggtgg cgggcggcct cagcggtctc gccagcgcga ccgacgcggt caacgtgaag
    25021 agcggcggcc gcctcatcct ggaggccgcc agcgccggca tcggcggaac gctggacccc
    25081 gacgggctgt tcctgacccc gttcggcatc cagctcacct cgcccgacgc cggcctgatc
    25141 ctgcgcgccg gtgggcacgc ctgggtcgcg gccgagtcgg gcctcttcgt cgacacggtg
    25201 ttcgcgctcg gcgacgtacg cctcgacgcc agcggcgcca tcctcgactt ccagctcacc
    25261 gaggcgcccc tggcgcccga gctcaatctg ctcgcgcgca atgtggcgct ggcttcgcgc
    25321 acgggggcga tcggctcgtc cgtcaatccg ttcgatgtcg cggtctcgca cgaggacggg
    25381 cgcatctccg ccagcaccgc tcgcttcggc gaaggtgcgc acctgaacgg gccgatgggc
    25441 gagttcttca acatcgccaa tgtcacgtcg ggcgacgccg tcgcgctgtc ctcggccacc
    25501 ttcatgttca tcgacggccg cgtcaccggg ccgggcccga tcagcctggt gtccggcgcc
    25561 agcatggtgc tgaccccgtt cgccgacatc cacgccacca ccctcggcgt gttcctgcag
    25621 gcttccgacc tgctgatgca ggaatcggcg gacggcgcag acgcggcgcg catccaggtc
    25681 gacgtcggca ccatcgacct gcgcaccgtg ggccaccagg ctgcacccga cgcgccccgc
    25741 agcggtggcg acgcggtcat caccggcatc ttcaccggca acccgaccga gagcgcgatc
    25801 ctcatcgacg ccttcggcag cgtgctcgac ggtggcgaca agcgcctcga catcatcgct
    25861 cgcaccgccc ccgccgccac cctgacgatc cgtgccggcg gccagatcgg cggcaacccg
    25921 ctcgagatcg acgtgctcca cttcaacgcc gactccggcg gcctcatcca cctcgactcg
    25981 ccgggttcgc tggccatggg tgacgtacgc gccgaacgcg agatcgacat ccgcgccgcc
    26041 ggctcgatca gtgcggactc cgtcaccagc gtgaccgagg acgtacggct cgcggccgat
    26101 ggcggcgacc tgcgcgtcgg cgccatcgcg ggacgcgacg tcgaactcgg cgttcagggt
    26161 ggcaatgcgg agatcggctc gatcgacgcg cgcgacctcg ggctccgggt ggagaacggc
    26221 gacgctcgca tcggttcgat cgacgcccgc gacgtcgacc tcggcgcgcg cgatcgcctc
    26281 gacatcgatc gtctcgacgc cggccgcagc ctgcgactgg gcggcaacga catcgtcgcc
    26341 cgggtcttcg ccagcggtgg cgcgccgatc gtcggctcca tcgggggcta tggcggcggg
    26401 atggcctcgg tcgcggacct gacgctgagc ggcccgggcg gattccactt cgacgacatc
    26461 gccgctcgaa ccggccggct cgacctgccc ctcggcaccc tgcaggtcga tcgcttccag
    26521 gtcggcgagc gcatggtcgt caccaatccg caaactcggc tgctgatcga ccagcagaac
    26581 ttccgcctgc agggctacga cgtgcagctc tactccggcg gggccccctt cgccctggcg
    26641 ctcgatcgca acctgatcgc caccgacagc ttcgtcatcg accgtcgcgc ctcgcacgag
    26701 gtgcgcggac ccgatggccc gctggcgagc gtggtcgagt acaccgagcg cgtgctcgcc
    26761 gacatccatc tcgccccgcc ggctgccgga gcgccccgcg aggacgggga agagagcctg
    26821 gtccgcatcg cccccacccc cgtcgcgctc ggaacggagg gagaatgagg ccgaaacgac
    26881 caatcccctc cagtggagag cccgcaatcg tgaaacccat gctgatcctg gctgcagcgc
    26941 tctcggcggc ccccgccctg gcccagaccc tgccctcggc ggcgaatccc ggcgcgttgc
    27001 agcagcgccg gatcgacgag gaggagcgcc gccgccagct cgaacagctc gagcgccgcg
    27061 agatcaccga tccggtccgg cgcgaagccg caccgccgcc ggctgccccc ccggcaggcg
    27121 aggcggtgcg cttcacggtg cgggagatcc gcttcaccga gtcggccatc ctgcccgccg
    27181 aggcgctcgc cgagctcgcc gggccctaca ccgggcgcga ggtgagcctc gccgagctgc
    27241 gcgagctcgc cgcgcgcatc gacgcggcct accgcgaacg gcgcgtggtc accgcgcgcg
    27301 cggtgatccc gccgcaggac gtctccgacg gtgtcgtcga catccgcctg gtggaaggcc
    27361 gcgtgggcga gatcgcggta gagggcaacg ccagcaccga cgcggaattc atcacccggc
    27421 gcatcggcct cgtccccggc gagctggtcg acctgccggt gctggagcag gacctgcgcc
    27481 gcttcaaccg cacccaggac gcgcagctgc gcgcggacct ggcggcgggg cagcgcttcg
    27541 gcacctcgga cctcaggatc ctggtcgagg agccgccgcg ccacagcctg cgcctgagcc
    27601 tcgacaacgg cggcagcgag ggctcgggcg agtggcgcac cggcctgcaa tacaccaacc
    27661 gcagcctgct cggcttccgc gacgagctga gcctgggcgt ggtcggcgcg aaggggcagg
    27721 agagctactc ggcaggctac agcttcccgg tgaaccgcag cggcggccgc ctcagcctgg
    27781 cggcgtacaa ggaccacgtc gagatcgtcg agggcccgct gcgcgacctc gacatcagcg
    27841 gccgctccac cggcaccatc gtcgcgctgc gccaaccggt gcatctgggc gaacgctcgc
    27901 gcaccgacct ggtgctgggc agcaaacggc gcgagacgcg caactggatc agcagcgtac
    27961 cgctgcagga gacgcagacg gtggacggcc acctcggcgt ggagcaccag gcggccgacg
    28021 accacggcta ctggctcgcc acctacaccc tcaccggcgg tcgcgccaag ggcttcgagc
    28081 gcagcaacta cacgctcggg cgcgccttcg tgcgccgcgt ccacgccctg gccccgggct
    28141 ggtcggcgca ggccggcctc aactaccagc acagcggcca cagcccgctg ccgagcggcg
    28201 agcagctgca gatcggcggc gagtacagcg tgcgcggcta cccggtgggc gtatggtcgg
    28261 gcgaccgcgg cttcatcgcc aacctggaac tgcatcaccc gctcggcgag ctcgccgcga
    28321 gcgaaggcct gcccggcgtg cgcgccaccg gcttcttctt cgccgaccac ggccgcgtca
    28381 cccccgaccg cgcacccaac tcgctcgaac cgaaccaccg ccagctcggc agcgcgggct
    28441 ggggcgtgcg cgccgcgctc ggcgagcggg tggacgcccg cctcaccttc gcccgcgcct
    28501 tcaaccgcct gcccgacgag gaccggcgct acgaggtgca tttcatgctg gtggcggagt
    28561 tcatgtagtc cacccgcatc cgtgccttcc gtcgctcctg cagggccccg ccatgcttcc
    28621 cgagactccg atgggagcgc cgcaagtcgc gatcgcggcg tacggacagg tcggtgcatg
    28681 ttccatgcgc cgcatcgcga cttgcgtcgc tcccacaaaa accatcgggg ccgcatcgcg
    28741 acttgcggcg ctcccacaaa aaccatcggg gccgcgtcgc gacttgcgtc gctcctacga
    28801 aaaccatcgg gtagggcgtg gcatgatcgg ggggtcagat ctcatccacc ccgcccccga
    28861 tgcaacttca cctgatccgc cacccccgcc cggccgtcga gccgggaatc tgctacggcc
    28921 agaccgacct cggcctggcc gaat
//
LOCUS       DAOQBS010000028        27886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1044_length_27886_cov_15.771119, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000028 DAOQBS010000000
VERSION     DAOQBS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 27886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27886
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1044_length_27886_cov_15.771119"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="PKN25_05210"
     CDS             <1..1647
                     /locus_tag="PKN25_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90332.1"
                     /translation="LLAAVGIPDPERRLQEFPFQFSGGMKQRVMIAIALAGRPRLLIA
                     DEPTTALDVTIQAQVLDLLAELQRREGMAMLLITHDLGVVARVAHRVGVMYAGQVVES
                     GPREAFFATPLHPYSRKLFAALPEAAKRGGALDAPVGSVPPPDRHFPGCRFAERCPAA
                     FDRCDEEVPVWHAVGTQAVRCHLYEGVAIPRRFVRETAGAADVVAARPAQSVLEVAEL
                     AVHFPVRKGFLRRTVGHVKAVDGVSLRLQPGRTLALVGESGCGKTTVGKALLRLIEPT
                     AGRLWLDGERYDALPLSALQPLRRAVQMVFQDPFASLDPRMRVGEIIEEGLAALGVGA
                     DHRARQARVTELLERVGLSAEMRWRYPHEFSGGQRQRIAVARALAVAPRVIVCDEPTS
                     ALDVSVQAQLLNLMRELQHEQGLAYLFITHNLAVVEYLADEVAVMYLGRIVEEGPIER
                     VLGVRAHPYTRALLAAVPRIEGAGGAAPDVGGTGVAGGSTATPGTIMAELPSPLAPPS
                     GCHFHPRCPYATDVCRIRYPAPADLGQGHRVRCHWPLALR"
     gene            complement(1805..3181)
                     /locus_tag="PKN25_05215"
     CDS             complement(1805..3181)
                     /locus_tag="PKN25_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNV90333.1"
                     /translation="MTSSASCAAQPRGGRRHGPLLAVCACALALAALASPASARDVTA
                     APATLTASDTAASAAVATSAPETARVLTLDLTRDANDIWDRIRRGFGMPDLDSQRVMD
                     QQSFYLNRPRFLKQVFERGGRYLYYIVDELERRGMPTELALLPMVESSYNPMAYSRAH
                     ASGLWQFIPSTGKHYNLTQDHWVDERRDVIASTNAALDYLQRIYEMHGDWHLALASYN
                     WGEGAVQRALKRNAEDGLPLEYEHLRMPDETRNYVPKLQAIKNIVAEPELFHFELPYV
                     ANELHFVSVEAPIGVDLATAAKLSGMPLDEFLALNPGFNRPAVTTPGQIFIVPTDRAE
                     QLQQGLAELDEEDGEWRMHVMAAGETLEAVAARHGLSSAELRRLNGLGPGSQPGEGHS
                     LLVPDGIDPDGALEVTSLLPFAATAARQPGLKLNTSQARSGEPHLKSPKARQRPGEKR
                     STKRSKKR"
     gene            complement(3188..3961)
                     /gene="gloB"
                     /locus_tag="PKN25_05220"
     CDS             complement(3188..3961)
                     /gene="gloB"
                     /locus_tag="PKN25_05220"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HNV90334.1"
                     /translation="MDIIPVPAFRDNYIWLVHDGRHALVVDPGDATPVEEALREHGLV
                     LGAILVTHHHPDHTGGIAALVASHRVPVYGPARESIAGVVHAVSDGDEIHLPAPAIAL
                     RVLDVPGHTAGHVAYVGMDIEPGLVFCGDTLFSAGCGRLFEGTPTQLAHSLARLAALP
                     PDTRVFCTHEYTLSNLAFARAAEPENVERDAWAAHCETLRARGEPTLPSTVARELAIN
                     PFLRCDQPGVIEAVAAHTGSRPQDALECLAALRAWKDRF"
     gene            3990..4715
                     /locus_tag="PKN25_05225"
     CDS             3990..4715
                     /locus_tag="PKN25_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV90335.1"
                     /translation="MSILRLEEWLQTPQGRYLLDWEQDGLDRLVADIFGYHAVQLGLS
                     GFDLLRANRMPRRIDCGPQGRDLRCSEYELPFATGSLDLVLLPHVLEFSAHPHRVLRE
                     VERVLVPEGSVVISGFNPLSLWGLRRLIARRGAEAPWSGHYRSAMRIRDWLNLLGFEI
                     QESRFGCYAPPLRTPQWLERWGCLDRAGSRWWPVLGAGYLLHGVKRVQGMRLITPHWR
                     GRKAAAKRLSPVAQRDGSTRNMQ"
     gene            4718..5176
                     /gene="rnhA"
                     /locus_tag="PKN25_05230"
     CDS             4718..5176
                     /gene="rnhA"
                     /locus_tag="PKN25_05230"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNV90336.1"
                     /translation="MEEVDIYTDGACSGNPGPGGWGAILRSGSHEKEIWGGEPATTNN
                     RMELLAVIRALDALKRPVAARVHTDSQYVQKGISEWIHGWKARGWKTASKEPVKNADL
                     WRALDDAASRHQVKWLWVRGHNGHPENERADALARRGVDAVRKSGAAVQC"
     gene            5190..5909
                     /gene="dnaQ"
                     /locus_tag="PKN25_05235"
     CDS             5190..5909
                     /gene="dnaQ"
                     /locus_tag="PKN25_05235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HNV90337.1"
                     /translation="MRQIVLDTETTGLDWRNGDRVIEIGCVELLDRSLTGRHYHVYIN
                     PGRGIDAEAVAVHGITEEFLADKPKFADIVADFEDFVRDAELVIHNASFDVGFLDAEL
                     ERLGRAKLGALCAGVVDTLKLAKEQNPGKKASLDALCDRYEIDNAHRELHGALLDAEL
                     LAEVFLAMTRGQESLMIGLDEEAAPRESDSEGAPVLRAAVRVLRASADECAAHDAALA
                     DIAKANKGLCLWLPPEPASAG"
     gene            complement(6110..6517)
                     /locus_tag="PKN25_05240"
     CDS             complement(6110..6517)
                     /locus_tag="PKN25_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV90338.1"
                     /translation="MLGSLLVKDYMVGDHLAFSPDTDLLKAVRLLLQRGLSGAPVVDE
                     NRRLIGFLSEKDCLKAALDASYFRRDEGKVEDFMNRDVVSIRASASLIDAIELFLARN
                     HHILPVVEGARLIGQISRHDILKGLEKVRRDER"
     gene            complement(6533..6736)
                     /gene="nqrM"
                     /locus_tag="PKN25_05245"
     CDS             complement(6533..6736)
                     /gene="nqrM"
                     /locus_tag="PKN25_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HNV90339.1"
                     /translation="MNTFLITLLVVGLVILAMAVGVLFGRKPISGSCGGLGSMGQDCE
                     FGCKKPCSKRQARMMAAQAEQPH"
     gene            complement(6867..7637)
                     /locus_tag="PKN25_05250"
     CDS             complement(6867..7637)
                     /locus_tag="PKN25_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNV90340.1"
                     /translation="MNPPIDISEEAGVRYLHFGSDWIQGAMRIRKPYALELAYTREMM
                     AGLLLRDGLAADGGHWPRKVLIIGLGAASLARFVHHHLPQARIRVVEIAPAVVAAARQ
                     FFKLPTEDARFSVHLGCGAQFVLEHDETWDYVLVDGFDRNARAGVLDTEPFHQAVRAR
                     LSARGIMAVNLFGRARGYKASLQRIVRAFDDRALAFPSCDSGNVVAFGAAGEPIERSV
                     EELRERARQLKAETGLDLLPTVTRLEAAGELPGGVLRM"
     gene            complement(7634..10243)
                     /gene="mutS"
                     /locus_tag="PKN25_05255"
     CDS             complement(7634..10243)
                     /gene="mutS"
                     /locus_tag="PKN25_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNV90341.1"
                     /translation="MMQQYLRIKQQHPDTLLFYRMGDFYELFFDDAEKAARLLDITLT
                     TRGQSGGKPIRMAGVPFHAVEQYLARLVKLGESVVIAEQVGEPGANKGPMERAVSRIV
                     TPGTLTDAALLDDRRDSLLLAASLHRGVLGLAWLNLANGDFRLMQCPSDALQAQFERL
                     RPAEVLVPDGLALPLLDTLAPALRRLADWQFDAETGTRLLTTHFGTRDLAGFGVEDLP
                     VALGAAAALYDYAQATQRQTLSHVTGLVVERESEYLRLDAATRRNLELTETLRGESSP
                     TLLSLLDTCVTSMGSRWLRHALHHPLRERAEPAARHAAVAELVGTVEGEMSEHTGAAP
                     GGFALGGRDGRIAFAVRSALRGVADVDRITARIALRSARPRDLSALRESLLRLPELAA
                     ALAPCRAPLLAEIVGALAIAPEPLALLQQAIAAEPAAMVRDGGVIAPGFDTELDELRG
                     IQTNCGEFLMALETRERERSGIPGLKVEFNKVHGFYIEVSRANADKVPDDYRRRQTLK
                     NAERYITPELKAFEDKALSANERALAREKTLYDQVLEVLAAHIPALQRIARALALLDG
                     LAALAEAALRYGYVQPRFLETPGLTITGGRHPVVERQVESFIRNDARLAATRRMLMIT
                     GPNMGGKSTFMRQVALICLLAHVGSFVPADAVELGPLDAIFTRIGASDDLASGRSTFM
                     VEMTEAAAILHGATERSLVLMDEIGRGTSTFDGLALAFAIARHLLEKSRALTLFATHY
                     FELTRLNADYPECANVHLDAVEHGHRIVFLHALEEGPASQSYGIEVAALAGIPAAVIR
                     DAKRRLRALENREIDAGPQADLFAALPEPEDAPLSHPVLSALADIDPDALSPREALER
                     LYALKRLAGDRNT"
     gene            10404..10925
                     /locus_tag="PKN25_05260"
     CDS             10404..10925
                     /locus_tag="PKN25_05260"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HNV90342.1"
                     /translation="MNTPDADTRRIGRRLGLLATLAFLALGWVSFDGALERREDPNRN
                     LVVAPGASELVLQRNRAGHYLLPGEINGRPVRLLLDTGATQVSVPARLAAELGLQAGA
                     PASVLTANGAVTVRQTRIPRLAFGPFEFRDVAGHLNPGMQGDEVLLGMSALRHLEFTQ
                     RGDTLTLRAPGSG"
     gene            complement(10947..11747)
                     /locus_tag="PKN25_05265"
     CDS             complement(10947..11747)
                     /locus_tag="PKN25_05265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNV90343.1"
                     /translation="MHPTLNIAVKAARRAASVINRASTQLDLLTVQSKSPNDFVTEVD
                     RAAEQAIIEVLRDAFPGHGILAEESGESGPESEYTWIIDPLDGTTNFIHGMPQYAVSI
                     AQAKNGVLEHAVVYDPNTNEMFTASRGAGAFLNDRRIRVSRRTRLNEALIGTGFPFRQ
                     FDHVDAYLAMFKELTQKTAGIRRPGAASLDLAYVASGRFDGFWEMGLSPWDMAAGVLL
                     IQEAGGLVSDLSGEANYLTTGNLVAGTPKIFGQLLPIIQAWRPANLRA"
     gene            11891..12628
                     /locus_tag="PKN25_05270"
     CDS             11891..12628
                     /locus_tag="PKN25_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNV90344.1"
                     /translation="MNRPLALDRIRVVLSRTTHPGNIGAAARAMKTMGLRELWLVAPE
                     SFPDEVATARASGAADLLDAARVVGTLEEALADTVFSAALTARRRELSLPRLQARDAA
                     LELVRRTQDGAVALVFGNETSGLTNEEAALCSLPVTIPTDAGFSSLNLGAAVQVLAYE
                     LRMAALGDAAAPADPQPEPATHADFEGFMAHLERAVTASGFHDPANPKRLLPRMRRLF
                     NRVRLEREEVALLRGMLTTFETPRRRG"
     gene            12711..14915
                     /locus_tag="PKN25_05275"
     CDS             12711..14915
                     /locus_tag="PKN25_05275"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNV90345.1"
                     /translation="MVSVTHAISQGDTAPPIDLLAAGLEPTEREHIEAALEWIADLYE
                     GKVLGTGEPTWTHAIGAALIAASLRLDAETRVAALLFAAWEELEAPAEEIEARFGPAI
                     TALVRGLHKLNGLRVLTRLTTSASAPEIRAQTEVLRKMLLAMVEDIRVVLVRLASRTQ
                     TLRFYTDLPGEARVEVARESLDIYAPLANRLGVWQIKWELEDLSFRFIEPDTYKRIAK
                     MLDERRVEREQFIRDSIERLKSELAAVGIEAEITGRAKHIYSIYNKMRKKRLDFSQVF
                     DIRALRVLVPEIKDCYTVLGIVHQMWQPIAKEFDDYITKPKGNNYQSLHTAVLAGDGR
                     ALEVQIRTFDMHKHAELGVAAHWRYKEGARQGGDYDEKIALLRSLLSWRDEVTDAAHW
                     VEQLKRASLDDTLYVLTPQGRVVDLPRGATPVDFAYRLHTDVGHHCRGAKVDGHLIPL
                     NTQLESGQTVEIVTAKEGGPSRDWLDTRQGYVATSRARSKIKQYFAQLDEEEILSRGR
                     SFVTKEMQRDGHGQANIDGLAERLGFKNAEALYLAAGRGEVGPRAMQTALREGSAPEP
                     AAEAEPEFVVSRSRSGDNQDKILIVGVGKLMTSLSRCCKPAPPDAIEGFVTRGRGISI
                     HRVDCADFQDLARKHPERVIPAEWGEKAHDTRNALYQVDINVQASDRQGLLRDISEVL
                     SREKLNVIAVNTLTKKGTAYMRFTMEVGGIKQVQRAITLVREVPGVIDAQRK"
     gene            complement(15030..17111)
                     /gene="ppk1"
                     /locus_tag="PKN25_05280"
     CDS             complement(15030..17111)
                     /gene="ppk1"
                     /locus_tag="PKN25_05280"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNV90346.1"
                     /translation="MPVDRMTLNYPPEHFLNRELSLLQFQRRVLAQAADPTVPLLERL
                     RFLCIVSSNLDEFFEIRVSGIKEQIRIGSRKSGEDGIAPVELLERVSDEVHEIISEQY
                     QLLNGDILPALEKEGVVFLRRSLWTEAQRAWIHDYFMREVMPVLTPIGLDPAHPFPRV
                     LNKSLNFAVELEGRDAFGRDSGAAIVQAPRALPRVIRLPRELCDQEYGFVFLSSVLHA
                     HVGELFSGMNVLGCYQFRVTRNSDLFVDEEEVKDLRASLKGELQQRHFGDAVRLEVAD
                     NCSEEMAGFLLQHFHLTPDDLYRTPGIVNLVRLMQVPDWVERPDLKYAPFVPGLPKVL
                     DKRRQVFTAIRGGDILLHHPFQSFAPVIEMLRAAADDPQVLAIKMTVYRTGTDSVLME
                     HLARAAQKGKEVTVVLELMARFDEEANITLANRLEEVGAHVVYGVFGYKTHAKLLMIV
                     RREEDGLRRYVHMGTGNYHPRTTRFYTDFGLLTCNAEIGEDVATVFKQLTGLGKATEL
                     RHLWQAPFTLQPNVVAAIRREAEIARAGRRGRVIAKMNALLEPETIEALYEASQAGVE
                     IDLIVRGPCALRPGVPGLSDNIRVRSVIGRFLEHHRIFHFHADGAEHMYLSSADWMDR
                     NFFRRIEIAFPVLDPRLKRRVMKEGLRPYLGDNCQAWDMQADGKYRRKHPRSIRRAAQ
                     LILLKELAASS"
     gene            17294..18793
                     /gene="ppx"
                     /locus_tag="PKN25_05285"
     CDS             17294..18793
                     /gene="ppx"
                     /locus_tag="PKN25_05285"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNV90347.1"
                     /translation="MQDLIAAIDLGSNSFRLQVGRIVNDQIYPLDGLKEAVRLAAGLS
                     PDKMLDLASQQRGVGALQRFHERLRDFPPTRVRAVATNTLRVAKNAPEFLIRAEAALG
                     FPIEIIAGREEARLIYVGVAHTLPDPHRQQLVVDIGGGSTEFIIGKSFESQLLESRYM
                     GCVGYSLRYFPEGRIDKRAFKEAELAARRELQTISAAYREAGWEEAVGSSGTAKAIVE
                     ILEQNGLSASGITREGLEKLKALLLRAGSLDNIDLAGLKGDRLPVILGGFAIMSAIFK
                     EFELEHMVFSEGALRLGVLYDLLGRYHHHDLRDATVSAFVRRYGVDLRQAQQVADTAC
                     HLLAQLEPETADPEHDDHRFLRWAAMLHEIGISVAHSSYHKHSAYIVGNADMPGFSRM
                     DQARLARLVLAHRGKLERVASIDPGSVDWLLIACMRLAVVIHRARDARGLPPVTLRRE
                     GRGFSLTAAPGWLQKLPLTAAALDEEQRQWQGLGRALYIRALSLRTPVA"
     gene            18800..19915
                     /locus_tag="PKN25_05290"
     CDS             18800..19915
                     /locus_tag="PKN25_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV90348.1"
                     /translation="MAGRVLDEVFTAGAEGIRLAGDWTLRGLDECLPTLRRALATVPP
                     DARWSLRAVRRMDSFGALLLWQAWGGRWPAALEADDAQRAIIGRIENASARALPEPPR
                     ASALDAVALLGKGLFAFAGHLSGFVALLGRLVLDLGHLLHHPRDWPLLEISANLHKVG
                     VKAMPVSALVGFLIGVVLSYLSALQLRTFGADAFIVNILGMGIIRELGPVLVSVLVAG
                     RSGSAMTAQLGVMRVTEEIDALAAMGVSRSLRLVLPKVVALSLAMPLLVLWTSAIALF
                     GGWVSAWVELGIGLQFFLRALPDAVPVANLYIGLAKGAVFGFLIALVACHFGLRVKPD
                     TESLSANTTASVVAAITVVILVDAVFAIATRSIGLPL"
     gene            19939..20703
                     /locus_tag="PKN25_05295"
     CDS             19939..20703
                     /locus_tag="PKN25_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV90349.1"
                     /translation="MVCLEGIVTRFGRNIVHEGLDLTIRAGDIVALVGGSGSGKTTLL
                     RHLVGLSRPAAGRVEVFGEALDAGGAAARLARQRRMGVLFQHGALFSAFSVAQNIAFP
                     LRELGVISRPEIDRLVALKLAMVEMEPAHAPLMPAELSGGMVKRVALARALALEPELL
                     LLDEPTAGLDPDRSASFIRLIRALHRELGLTVVLVTHDLDTLAQMATSVAVLAERRIV
                     SHASLADTLAYDHPFVARFFSGLQARCGQPRGAGAG"
     gene            20705..21622
                     /locus_tag="PKN25_05300"
     CDS             20705..21622
                     /locus_tag="PKN25_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNV90350.1"
                     /translation="MENRAHALAAGLFAILLGAGVVFAIWWFSGQRVPMREIVLEARG
                     DINGLGAQSRVRYRGMAVGSVRAVAIDPEDLRTLLVRIAVPADLPLTRGTTAALGTLG
                     VTGLAFVQLDDRGVDRRPLAAADGEVPRIALQPGLVEALSGRALAALDQFQALGERLQ
                     GALASLESAAAGVDRGVAELPATLAALRAALSAGNIARITSVLGNLERGSAEAVPAVA
                     ELRRLIVRIDQAAARLELRAAAAGDDLVERTLPQLDVLLGELTSTSQRFGHLVEELDA
                     SPQLLLTGRDRPLPGPGEAGHEGFAGSDR"
     gene            21640..22278
                     /locus_tag="PKN25_05305"
     CDS             21640..22278
                     /locus_tag="PKN25_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNV90351.1"
                     /translation="MQPRHPDCHRVLPRLSACIATALLVAACQVLPERPRAATTYDLG
                     TGERAALAHGLAPARVAVSSPPWLQTTTMHYRDARVDPGRRRAYADNRWAAPPPAMLG
                     LVLERTLGAGEGGGCRLQVQLDEFEQVFTAGERSEARLLARFALLPVRGAAVIASASL
                     VVAEPATTADASGGASAFRAATERLALAIAQWIAETDRALSGGLGIAGRCQR"
     gene            complement(22351..24042)
                     /locus_tag="PKN25_05310"
     CDS             complement(22351..24042)
                     /locus_tag="PKN25_05310"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV90352.1"
                     /translation="MNLRTRFLLLISALIVVGSVSAWAVFHHLARQIVMQWGQRVADI
                     QVRYDRARLLQPLEREIALARQMADSLVLKRWARSPVDPALEAEAVREMENFRRNFSS
                     GNYFVALRTSGAYYHNNAGDEFAGRQLRYHLDPAKPADAWFYKLVQVGPDFHLNVNPD
                     AELGVTKLWIDVLIRDGDEILGVVGTGIELTRFLDEIVDLHQDGVSTLFADLSGAIQL
                     HRDAKLIDFASLVKPEGQKSLADQLLDRESDRVALRETMSALAASGDGVPRVLDRFVE
                     SGGRRTLLGIAFVPEIGWYEITLLDLDVLMPVAALTPVAVAFVLTLLVALVLVHLVLR
                     RLILDPLGRLEQAMLRVRDGDLSPAPLAQASGEIGRLTQHFEAMAGAIRAHTAELEAK
                     VRERTDALHRLARIDPLTNLTNRRGMSEMLEAEIIRAERQGSRFGLLWLDVDHFKEIN
                     DRRGHGAGDEALTRLARVLEGCLRPYDRAARWGGDEFLVLLSPCDAATLEAIAHRVRA
                     DIEHGSAACGERVTVTIGACLAEPGETLGVVLQHADDALYRAKDAGRNQVVIAAR"
     gene            24266..25075
                     /locus_tag="PKN25_05315"
     CDS             24266..25075
                     /locus_tag="PKN25_05315"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNV90353.1"
                     /translation="MNAEFRPDAAAEAPILRREDAGGVTTLTLNRPQQFNALSFEMLE
                     ALIAAVDEIARDETVRVVVIAGEGKAFCAGHDLKEMRGNHTLEFQQRLFRLCGRFMMK
                     LTELPQPVIARVHSIATAAGCQLVSMCDLAVAAEHARFAVSGINVGLFCATPGVGLSR
                     NMGRKEAFEMLVTGDFIDAREAQRRGLINRVVPADQLDEEVGRLAASILAKSPVAIRM
                     GKQMFYKQLEMGLDAAYQLASETMACNAMCEDAAEGIDAFIAKRKPEFKGR"
     gene            25119..25895
                     /locus_tag="PKN25_05320"
     CDS             25119..25895
                     /locus_tag="PKN25_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNV90354.1"
                     /translation="MLIDTHVHLDAAEFDDDREQVIARARAAGVGRFVVPAVERRGFD
                     AVERLADDHRGIVHALGIHPLYTMRAAREDLELLDQRLNRGRAIAVGEIGLDHYVTDV
                     DRGVQLEFFTAQLRLAARHGLPVILHVRRAIDPILAQLRRIPVPGGIAHAFNGSRQQA
                     DILIGMGFKLGFGGSMSYGGSTRIRELAASLPLEAIVLETDAPDIAPEWARGRRNEPA
                     NLARYAGILAELRGMAPEEVADVTTANAIQVLHWPPLEEE"
     gene            26127..26999
                     /locus_tag="PKN25_05325"
     CDS             26127..26999
                     /locus_tag="PKN25_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV90355.1"
                     /translation="MKNGMALLAAIGIATLSASAQAALIRLEVSGNLAYGTDRTNVFG
                     LGANTSLTGRAATFSWLMDSASLGSDRNTDPTLSAYGCGSAAPGCTYFMSASVTINGV
                     TRTLAGRGSDLTNDVRMYADTNGPGLGSADGYYLRMGQTRDVGNWYLDEGGNWYRAAT
                     RITSKVEIGVNDAVDSILAGLDPAELHGWSTSLLTGTNDDGSTVFVFSETDLNGVQIF
                     EYATGGLLLTDRSTRWWTDTAPSSGSGTGGTNNPGGGTNAPAVTVPEPATIALLGLGL
                     GLLRRKSRRHSGRA"
     gene            complement(27058..27651)
                     /locus_tag="PKN25_05330"
     CDS             complement(27058..27651)
                     /locus_tag="PKN25_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV90356.1"
                     /translation="MENIVKKPRTQLDRAAWIAGATEVLAEEGIAGLRVEVLAKRLKV
                     TKGSFYWHFTDRRDLLMAVLAQWKEGRIRDIIKQTRAQPGRELEQIYHVIDVYSASRS
                     RRGMMIELAVRDWARRDAEAAAIVAEVDDVRLRCARDLFLACGVPMDEASSRCMLLYA
                     YVFGVSLMIYEKFDRDVVRLKRDIADLIARSPSRVVA"
BASE COUNT         4105 a         9423 c        10045 g         4313 t
ORIGIN      
        1 ctgctcgccg cggtgggcat ccccgacccc gagcgccggc tgcaggagtt tcccttccag
       61 ttctcgggcg ggatgaagca gcgcgtgatg atcgcgatcg cgctcgccgg ccgtccgcgc
      121 ctgctgatcg ccgacgagcc gaccaccgcc ctcgatgtca ccatccaggc ccaggtgctc
      181 gacctgctcg ccgagttgca gcgccgcgag ggcatggcga tgctgctgat cacccacgat
      241 ctcggcgtgg tcgcgcgcgt cgcccaccgc gtgggggtga tgtatgccgg tcaggtggtg
      301 gagagcggtc cgcgcgaggc cttcttcgcc acgccgctgc atccgtattc gcgcaagctc
      361 ttcgccgcgc tgcccgaggc ggccaagcgc ggcggtgcgc tcgacgcgcc cgtgggcagc
      421 gtgccgccgc ccgaccgcca cttccccggc tgccgcttcg ccgagcgctg tccggccgcc
      481 ttcgaccgct gcgacgagga agtccccgtc tggcatgcgg tcggcacgca ggccgtgcgc
      541 tgccatctgt acgaaggcgt tgcgattccg cgccggttcg tgcgcgagac ggcgggtgcg
      601 gccgatgtcg tcgccgcacg cccggcgcag agcgtgctcg aggtcgccga gctggcggtg
      661 catttcccgg tacgcaaggg tttcctgcgc cgcaccgtcg gtcacgtgaa ggccgtcgac
      721 ggcgtgagcc tgcgcctgca gcccgggcgc acgctcgcgc tggtgggcga atcgggctgc
      781 ggcaagacca ctgtgggcaa ggccctgctg cgcctgatcg agcccaccgc cggccgccta
      841 tggctcgatg gcgagcgcta cgacgcactg ccgctctcgg cgctgcaacc cctgcggcgt
      901 gcggtgcaga tggtcttcca ggaccccttc gcctcgctcg atccgcgcat gcgggtgggc
      961 gagatcatcg aggagggcct ggctgcgctt ggcgtcggcg ccgaccaccg cgcgcgccag
     1021 gcccgcgtga ccgaactgct cgagcgtgtg gggctgtcgg cggagatgcg ctggcgctat
     1081 ccgcacgagt tctccggcgg ccagcgccag cgcatcgcgg tggcgcgtgc gctcgcggtg
     1141 gcgccgcggg tgatcgtgtg cgacgagccc accagcgcgc tcgacgtttc ggtgcaggcg
     1201 cagctgctca acctgatgcg cgagctgcag cacgagcaag gcctcgccta cctcttcatc
     1261 acccacaacc tcgcggtggt ggagtacctc gccgacgagg tcgcggtgat gtatctcggt
     1321 cgcatcgtcg aggagggccc gatcgagcgc gtgctcggcg tgcgcgccca cccctacacc
     1381 cgcgcgctgc tcgcggcggt gccgcggatc gagggcgcgg gcggcgcggc accggacgtg
     1441 gggggcacag gcgttgcggg cgggagcacg gcgacccccg gcacgatcat ggccgagctg
     1501 ccgtcgccgc tcgccccgcc gagcggctgc catttccatc cgcgctgccc atacgccacc
     1561 gacgtatgcc ggatccgcta ccccgcgccc gccgacctcg gccagggcca ccgcgtgcgc
     1621 tgccactggc cgctcgcgct ccgctgacac cggttttctt gcaggagcga cgcgcgtcgc
     1681 gatcgcgcga gcgttcaagc gcctgcgctc gtcgaggcgc tgcagtcgcg actggcgtcg
     1741 ctccttgtgc agggggcgcg aagcgcggtg ggagccccgg gactccatgc ccccgccgcc
     1801 aggatcagcg cttcttgctg cgcttggtgc tgcgcttctc gcccgggcgt tgcctggcct
     1861 tgggcgactt gaggtgaggc tcgccgctgc gggcctggct ggtgttgagc ttgaggcccg
     1921 gttggcgggc ggcggtggcg gcgaacggga gcaggctggt cacctcgagc gcaccgtccg
     1981 ggtcgatgcc gtcgggcacc agcagcgaat ggccttcgcc cggctggctg ccggggccga
     2041 ggccgttgag cctgcgcagc tcggccgagc tcaggccatg gcgggcggcg accgcctcga
     2101 gggtctcgcc cgcggccatc acgtgcatcc gccattcgcc gtcctcctcg tcgagctcgg
     2161 cgaggccttg ctgcagctgc tcggcgcggt cggtgggcac gatgaagatc tgcccgggcg
     2221 tggtcaccgc ggggcggttg aagcccgggt tgagggcgag gaactcgtcc agcggcatgc
     2281 cggagagctt cgccgcggtg gcgaggtcga ccccgatcgg tgcctcgacg ctgacgaagt
     2341 gcagctcgtt ggcgacgtag ggcaactcga agtggaagag ctcgggctcg gcgacgatgt
     2401 tcttgatcgc ctgcagcttg ggcacgtagt tgcgcgtctc gtcgggcatg cgcaggtgct
     2461 cgtattcgag cggcaggccg tcctcggcgt tgcgcttgag cgcgcgctgc accgcacctt
     2521 cgccccagtt gtaggaggcg agcgcgaggt gccagtcgcc gtgcatctcg tagatgcgct
     2581 gcaggtagtc gagcgcggcg ttggtcgagg cgatcacgtc gcggcgctcg tccacccagt
     2641 ggtcctgggt gaggttgtag tgcttgccgg tcgaggggat gaactgccac aggccggaag
     2701 cgtgcgcgcg cgagtaggcc atcgggttgt agctgctctc gaccatcggc agcagcgcga
     2761 gctcggtcgg catgccacgg cgctcgagtt cgtcgacgat gtagtagaga tagcgcccgc
     2821 cgcgctcgaa gacctgcttg aggaagcgcg ggcggttgag gtagaagctc tgctggtcca
     2881 tcacccgctg gctgtcgagg tcgggcatgc cgaagccgcg gcggatgcga tcccagatgt
     2941 cgttggcgtc gcgggtgaga tcgagcgtga gcacgcgcgc cgtctcgggc gcggacgtcg
     3001 ctaccgcggc gctagcggcg gtgtcgctgg cggtgagggt ggccggggcc gccgtcacgt
     3061 cacgggcgga cgcgggagag gcgagggcgg caagggcgag ggcgcaggcg cagacggcca
     3121 gcagcgggcc atgcctgcgg ccgccgcggg gctgggcggc acaagaagcg gaagaggtca
     3181 tcggggctca gaagcggtcc ttccaggcac gcagcgcggc gaggcattcg agcgcatcct
     3241 gcgggcggct gccggtgtgc gcggcgacgg cctcgatcac cccgggctgg tcgcagcgca
     3301 ggaaggggtt gatcgccagc tcgcgggcga cggtgctcgg cagggtgggt tcgccgcgtg
     3361 cgcgcagcgt ctcgcagtgt gcggcccagg cgtcgcgttc gacgttctcg ggctcggccg
     3421 cgcgtgcgaa ggccaggttg gacagcgtgt attcgtgggt gcagaacacc cgggtgtcgg
     3481 ggggcagggc ggccaggcgg gcgagcgagt gcgcgagctg ggtcggcgtg ccttcgaaga
     3541 gccggccgca gccggcggaa aagagcgtat ccccgcagaa caccaggccc ggctcgatgt
     3601 ccatgccgac gtaggccacg tggccggcgg tgtgcccggg cacgtcgagc acgcgcagcg
     3661 cgatggcggg cgcgggcagg tggatttcgt cgccgtcgga gacggcatgg acgacgcccg
     3721 cgatcgattc gcgcgcgggg ccgtaaaccg gtacacggtg gctcgccacc agcgcggcga
     3781 tgccgccggt gtggtcgggg tggtggtggg tgaccaggat cgcgccgagc acgaggccgt
     3841 gctcgcgcag ggcttcctcg accggggtcg cgtcgcccgg atcgaccacc aaggcatggc
     3901 ggccgtcgtg gacgagccag atatagttat cgcggaaggc gggaacgggg ataatgtcca
     3961 tcccgcgaat tgtgaggacc tgagtgccga tgtcaatcct ccggctcgag gaatggctgc
     4021 agaccccgca ggggcgctac ctgctcgact gggagcagga cggactggac cgcctggtgg
     4081 cggacatttt cggctatcac gcggtgcagc tcgggctgtc cggattcgat ttgctgcgcg
     4141 ccaatcgcat gccgcgccgg atcgattgcg gcccgcaggg tcgcgacctg cgctgcagcg
     4201 aatacgagct gccctttgcc accggcagcc tcgaccttgt gctgctgccg catgtgctcg
     4261 agttttccgc gcacccgcac cgggtgctgc gcgaggtcga gcgcgtgctg gtgcccgagg
     4321 gcagcgtcgt gatcagcggc ttcaacccgc tcagcctgtg gggcctgcgt cgcctgatcg
     4381 cgcgccgcgg tgcggaggcg ccgtggagcg ggcattaccg ctcggcgatg cgcatccgcg
     4441 actggctcaa cctgctcggc ttcgagatcc aggagagccg cttcggctgt tacgccccgc
     4501 cgctgcgcac gccgcagtgg ctggagcgct ggggctgcct cgaccgggct ggctcgcgct
     4561 ggtggccggt gctgggcgcg ggctatctgc tgcacggggt caagcgggtg cagggcatgc
     4621 ggctgatcac cccccactgg cgcggccgca aggccgcggc caaacgactt tcgccggtcg
     4681 cccagcgcga cggctcgacg aggaacatgc agtaacgatg gaagaagtgg atatctatac
     4741 cgacggcgcc tgcagcggca atcccggacc cggcggctgg ggcgcgatcc tgcgctccgg
     4801 atcgcacgag aaggagatct ggggtggcga gccggcgacg accaacaacc gcatggagct
     4861 gctcgcggtg atccgtgccc tcgacgcgct caagcgcccg gtggcggcgc gagttcacac
     4921 cgacagccag tacgtgcaga aggggatctc ggagtggatc cacggctgga aggcgcgggg
     4981 ctggaagacg gcctcgaagg agccggtcaa gaacgccgac ctgtggcggg cgctcgacga
     5041 cgcggcatcg cgccaccagg tgaagtggtt gtgggtgcgc ggccacaatg gccaccccga
     5101 gaacgaacgc gccgacgcgc tcgcgcgccg cggcgtggac gcggtgcgca agtccggcgc
     5161 cgcggtgcaa tgctgagcgg gagcgtgcaa tgagacagat cgtcctcgac accgaaacca
     5221 cgggcctcga ctggcgcaac ggcgaccgcg tcatcgagat cggctgcgtc gaactgctcg
     5281 accgcagcct caccggccgc cactaccacg tctacatcaa ccccgggcgc ggcatcgacg
     5341 ccgaggcggt ggcggtgcac ggcatcaccg aggaattcct cgccgacaag ccgaagttcg
     5401 ccgatatcgt cgccgacttc gaggacttcg tgcgcgacgc cgagctggtg atccacaacg
     5461 cgagcttcga cgtcggcttc ctggatgccg aactcgagcg cctcgggcgc gcgaagctgg
     5521 gtgcgctgtg cgccggggtc gtcgatacgc tgaagctcgc caaggagcaa aacccgggca
     5581 agaaggcttc gctcgacgcg ctctgcgacc gctacgagat cgacaacgcg caccgcgaac
     5641 tgcacggcgc gctgctcgac gcggaactgc tcgccgaggt cttcctggcg atgacgcgcg
     5701 gccaggaaag tctcatgatc gggctcgacg aggaagccgc gccgcgggag tccgacagcg
     5761 agggcgcgcc cgtgctgcgc gccgcggtcc gggtgttgcg tgcgagcgcc gacgaatgcg
     5821 ccgcccacga tgcagcgctc gccgacatcg caaaggcgaa caaggggctg tgcttgtggt
     5881 tgcctccgga gccggcgtcg gcgggatgag cgcctggcgg ctgggcccgc cgtgacttgc
     5941 cacgcccctg cagatgcggg agcgtgcgcc ggcgctcatg gaaggcgatg caagtcgtga
     6001 tcagggcgca tgggatctgc gacggcgcca tggaagcgcc cgctcgcgac tcgcatcgct
     6061 ccgcaggtgc cccgcagcgc accggtttcg tgcacgagga cgctgaaggc tagcgttcgt
     6121 cgcggcggac cttctccagg cccttgagga tgtcgtggcg ggaaatctgc ccgatcaggc
     6181 gcgcgccctc gacgaccggc aggatgtggt ggttcctggc aaggaagagt tcgatcgcgt
     6241 cgatcaggct ggcactggcg cggatcgaga caacgtcgcg gttcatgaag tcttccacct
     6301 tcccttcgtc gcggcggaaa tacgaggcgt ccaacgccgc cttgaggcag tccttctcgg
     6361 agaggaagcc gatgaggcgt cggttttcgt cgaccaccgg cgctccggag aggccgcgct
     6421 ggagcagcag acggaccgcc ttgagcagat cggtgtccgg cgagaaggcg aggtggtcgc
     6481 cgaccatgta gtccttgacg agaagggagc cgagcatcat gtctccgggg aatcagtgtg
     6541 gttgttcggc ctgcgccgcc atcatgcggg cctggcgctt cgagcacggc ttcttgcagc
     6601 cgaactcgca gtcctgaccc atcgagccga gcccgccaca gctgccggag atcggcttgc
     6661 gcccgaaaag gacgccaacc gccatggcca ggatcacgag gccgacgacg agcagggtga
     6721 tcagaaaagt gttcatggtt tgcagcttat cgggttgcgg atgcacggac atccgatcgg
     6781 gtggacaata acgcgggctg ccgggttctg gccatggggc cgttcgagca cgcgcgccgg
     6841 atcgacgcaa gtcgcgagcg ggcggctcac atgcgcagca cgccgcccgg gagttccccc
     6901 gcggcttcga ggcgcgtcac tgtaggcagg aggtcgagtc cggtctcggc cttgagctgg
     6961 cgggcgcgtt cgcgcagttc ctcgacgctg cgttcgatcg gctcgccggc cgcgccgaag
     7021 gcgaccacgt tgccgctgtc gcaggacgga aaggcgagcg cgcggtcatc gaaggcgcgc
     7081 acgatgcgct gcaggctggc cttgtagcca cgcgcgcggc cgaagaggtt caccgccatg
     7141 atgccgcgcg ccgacaagcg cgcacgcacg gcctggtgga agggctcggt gtcgagcacg
     7201 ccggcgcgcg cattgcggtc gaagccatcg accagcacgt agtcccaggt ctcgtcgtgc
     7261 tcgagcacga actgggcgcc gcagccgagg tgcaccgaga agcgcgcatc ctccgtcggc
     7321 agcttgaaaa actggcgtgc cgcggccacc accgcgggag cgatctcgac cacgcggatc
     7381 ctggcctgcg gcaggtggtg atgcacgaag cgcgccagcg aggccgcgcc gaggccgatg
     7441 atcagcacct tgcgcggcca gtgcccgccg tcggcggcaa ggccgtcgcg cagcagcagg
     7501 ccggccatca tctcgcgggt gtaggcgagt tccagtgcgt acggcttgcg gatgcgcatc
     7561 gcgccctgga tccagtcgga gccgaagtgc aggtagcgca cgccggcttc ttcggagatg
     7621 tcgatcggcg ggttcatgtg ttgcggtctc cggccaggcg cttgagggcg tagaggcgtt
     7681 ccagcgcctc gcgcgggctc agtgcatcgg ggtcgatgtc ggcgagcgcg ctgagcaccg
     7741 ggtgcgacag cggcgcgtcc tccggctcgg gcagggcggc gaagaggtcg gcctgcgggc
     7801 cggcgtcgat ctcgcggttc tccagcgcgc gcagccggcg cttggcgtcg cggatcaccg
     7861 cggccgggat gccggcgagc gcggcgacct cgatgccgta gctctggctc gccgggcctt
     7921 cctcgagcgc gtgcaggaag acgatgcggt ggccgtgctc gaccgcgtcc agatgcacgt
     7981 tggcgcactc gggatagtcg gcgttgagcc gggtgagctc gaaataatgc gtggcgaaca
     8041 gtgtcagcgc gcgcgacttc tccagcaggt ggcgggcgat cgcgaacgcg agcgcgaggc
     8101 cgtcgaaggt cgaggtgccg cggccgatct cgtccatcag caccaggctg cgctcggtgg
     8161 cgccgtgcag gatggcggcg gcctcggtca tctcgaccat gaaggtcgag cgccccgagg
     8221 cgaggtcgtc ggaggcgccg atgcgagtga agatggcgtc gagcgggccg agctcgacgg
     8281 catccgccgg cacgaagctg ccgacatggg cgagcaggca gatcagcgcg acctggcgca
     8341 tgaaggtcga tttgccgccc atgttggggc cggtgatcat cagcatgcgg cgcgtggcgg
     8401 ccaggcgggc gtcgttgcgg atgaagctct cgacctggcg ctcgaccacc ggatggcggc
     8461 cgcctgtaat ggtgagcccg ggtgtctcca ggaagcgtgg ctgtacgtag ccgtaacgca
     8521 gcgcggcctc ggccagcgcg gcgaggccgt cgagcagggc cagcgcgcgc gcgatgcgct
     8581 gcagcgccgg gatgtgggcg gcgagcacct cgagcacctg gtcgtagagc gtcttctcgc
     8641 gcgccagcgc acgctcgttg gccgacagcg ccttgtcctc gaaggccttg agctcgggcg
     8701 tgatgtagcg ctcggcgttc ttcagcgtct ggcggcggcg gtagtcgtcg ggcaccttgt
     8761 cggcgttggc gcggctgacc tcgatgtaga agccgtgcac cttgttgaac tcgaccttga
     8821 gcccggggat gccgctgcgc tcgcgctcgc gggtctccag cgccatgagg aattcgccgc
     8881 aattggtctg gataccgcgc agttcgtcga gctcggtgtc aaagccgggg gcgatcacgc
     8941 cgccgtcgcg caccatggcg gcgggctcgg cggcgatggc ttgctgcagc agggcgagcg
     9001 gctcgggggc gatggcgagc gcgccgacga tctcggcgag cagcggcgcc cggcagggtg
     9061 cgagcgcggc ggcgagctcg ggcagccgca gcaggctctc gcgcagcgcc gagaggtcgc
     9121 gcgggcgcgc gctgcgcagc gcgatgcggg cggtgatgcg gtcgacgtcg gccacgccgc
     9181 gcagcgcgct gcgcaccgcg aaggcgatgc ggccgtcgcg gccaccgagc gcgaagccgc
     9241 cgggggctgc gccggtgtgc tcgctcatct cgccttcgac cgtacccacc agctccgcca
     9301 ccgcggcatg gcgcgcggcg ggctcggcgc gctcgcgcag cgggtggtgc aacgcatggc
     9361 gcagccagcg cgagcccatg ctggtcacgc aggtgtcgag cagcgacagc agggtgggcg
     9421 aggactcgcc gcgcagggtc tcggtgagct ccaggttgcg ccgggttgcg gcgtccaggc
     9481 gcagatattc cgactcgcgt tcgaccacga ggccggtgac gtgggagagc gtctggcgct
     9541 gggtggcctg ggcgtagtcg tacaaggccg cggcggcgcc gagtgccacc ggcaggtcct
     9601 cgacgccgaa gccggcgagg tctcgcgtgc cgaagtgggt cgtcagcagc cgcgtgccgg
     9661 tctcggcgtc gaactgccag tcggccaggc ggcgcagcgc gggggcgagg gtgtcgagga
     9721 gcggcagggc gaggccgtcg ggcaccagca cctcggccgg gcgcaggcgc tcgaactgcg
     9781 cctgcagtgc gtccgatggg cactgcatga ggcggaagtc gccgttggcg agattcagcc
     9841 aggccagccc gagcacgccg cggtgcaggc tggcggcgag cagcaaggag tcgcggcggt
     9901 cgtcgagcag cgcggcgtcc gtgagcgtac cgggggtgac gatgcggctc accgcgcgct
     9961 ccatcggccc cttgttcgcg cccggctcgc ccacctgctc ggcgatcacc accgattcgc
    10021 cgagcttgac caggcgcgcg aggtattgct cgaccgcgtg gaagggcacg cccgccatgc
    10081 ggatcggctt gccgcccgac tggccgcggg tggtgagcgt gatgtcgagc aggcgcgcgg
    10141 ccttctcggc gtcgtcgaag aagagctcgt agaagtcgcc catgcggtag aagagcaggg
    10201 tgtccggatg ctgctgcttg atccggaggt actgctgcat catcggagtg tgggcgccga
    10261 tctcggcctc gctcagcgcg gcggggcgga tggccgtgtc gccgaagggc ggcttgcgga
    10321 ttgccaactg aagaaacctc ccggttgccg tgctgtcgtc agcaaaggca ggattatcac
    10381 cgtggatcga aggaaagacc gacttgaaca cacccgacgc cgacacccgc cgcatcggcc
    10441 gcaggctggg cctgctggcg acgctcgcct ttctcgcgct gggctgggtg agcttcgacg
    10501 gcgcgctcga gcgccgcgag gaccccaacc gtaatctcgt ggtcgccccc ggcgccagcg
    10561 agctggtgct gcagcgcaac cgcgccgggc actacctgct gcccggcgag atcaacggcc
    10621 gtccggtgcg cctgctgctc gacaccggcg cgacgcaggt gtcggtccct gcgcgcctcg
    10681 ccgcggagct tgggctgcag gcgggcgcgc cggcctcggt gctgaccgcc aacggcgcgg
    10741 tcacggtgcg ccagacgcgc atcccgcgcc tcgccttcgg ccccttcgag ttccgcgacg
    10801 tggccggcca cctgaacccg ggcatgcagg gcgacgaggt gctgctcggc atgtcggcgc
    10861 tgcgtcacct ggagtttacc cagcgcggcg acacgctcac cctgcgcgcg ccgggttccg
    10921 gctagcgggc cgcgttttct cggtcctcag gcccgcaggt tggccgggcg ccaggcctgg
    10981 atgatcggca gcagctggcc gaagatcttc ggcgtgccgg caaccaggtt gccggtggtg
    11041 aggtagttgg cttcgcccga caggtcggac accaggccgc cggcctcctg gatcagcagc
    11101 acgccggccg ccatgtccca gggcgacagg cccatctccc agaagccgtc gaagcggccg
    11161 gacgccacgt aggcgaggtc gagcgaggcg gcgcccgggc ggcggatgcc ggcggtcttc
    11221 tgggtgagct ccttgaacat cgccaggtag gcgtcgacgt ggtcgaactg gcggaagggg
    11281 aagccggtgc cgatcagcgc ctcgttgagg cgggtgcggc gcgagacgcg gatgcggcgg
    11341 tcgttgagga aggcgccggc gccgcgcgaa gcggtgaaca tctcgttggt gttggggtcg
    11401 tagaccaccg cgtgctcgag cacgccgttc ttcgcctgcg cgatcgagac cgcgtactgt
    11461 ggcatgccgt ggatgaaatt ggtggtgccg tcgagcgggt cgatgatcca ggtgtattcg
    11521 ctctcgggtc cggactcgcc ggactcctct gctagaatcc cgtgccccgg gaaggcgtcg
    11581 cgcagcacct cgatgatcgc ctgttcggcg gcgcggtcga cctcggtgac gaagtcgttg
    11641 ggcgacttgg actggacggt cagcagatcg agctgggtcg acgcgcggtt gatgacggac
    11701 gcggcgcgac gggcggcctt gacggcgatg ttcagggtgg gatgcatgcg gagcggtctc
    11761 ggttaaagaa tcggggcctg ccacgtgcct cgggcgcgcg ccgccgcccg cttcatcgag
    11821 ccgccgcgcg accgccgggc cacgttccgg gcaggcagcc cggcaaattc ggaaaacgcg
    11881 gaattttaat atgaatcgcc ccctcgcgct cgaccgcatc cgcgtcgtgc tgtcccgtac
    11941 cacccatccc ggcaacatcg gcgccgccgc gcgcgcgatg aagaccatgg gcctgcgcga
    12001 gctctggctg gtcgcgcccg agtcctttcc cgacgaggtc gccaccgcgc gcgcctcggg
    12061 cgcggccgac ctgctggacg ccgcgcgggt ggtcggcacg ctggaggagg cgctcgccga
    12121 cacggtcttt tccgcggcgc tgaccgcgcg ccggcgcgaa ctctcgctgc cgcgcctgca
    12181 ggcgcgcgac gcggcgctgg agctggtgcg gcgcacgcag gacggcgccg tcgcgctggt
    12241 gttcggcaac gagaccagcg ggctcaccaa cgaggaggcc gccttgtgca gcctgccggt
    12301 gaccatcccc accgacgccg ggttctcctc cctgaacctc ggcgcggcgg tgcaggtgct
    12361 ggcctacgag ctgcgcatgg ccgcgctcgg cgacgccgcc gcgcccgccg acccgcagcc
    12421 cgagccggcc acgcatgccg acttcgaggg cttcatggcg cacctcgagc gtgcggtcac
    12481 cgccagcggc ttccacgatc ctgccaaccc caagcgcctg ttgccgcgca tgcggcgctt
    12541 gttcaaccgc gtgcgcctcg agcgcgagga ggtcgccctg ctgcgcggca tgctcaccac
    12601 cttcgagacg ccgcgccggc gcggctgacc cggggagtcg cacccggcga aggggctgcg
    12661 agcacgggca tgcagcacga tggactgccc gatgtgggat aattgcaccc atggtatccg
    12721 tcacccacgc catctcccag ggcgacaccg cccctccgat cgatctgctc gcggccggcc
    12781 tggagccgac cgaacgcgag cacatcgaag ccgcgctcga atggatcgcc gacctctacg
    12841 agggcaaggt gctcggcacc ggcgagccga cctggacgca cgcgatcggt gccgcgttga
    12901 tcgcggcctc gctgcgtttg gacgccgaga cgcgcgtggc cgcgctgttg ttcgcagcct
    12961 gggaggaact cgaggccccc gccgaggaga tcgaggcgcg cttcggtccg gcgatcaccg
    13021 cgctggtgcg cggcctgcac aagctcaacg gcctgcgcgt gctcacccgc ctcaccacca
    13081 gcgcgagcgc gcccgagatc cgcgcgcaga ccgaagtcct gcgcaagatg ctgctggcga
    13141 tggtcgagga tatccgcgtc gtgctggtgc ggctggcctc gcgcacccag accctgcgct
    13201 tctacaccga cctgccgggc gaggcccgcg tcgaggtcgc gcgcgagagc ctggacatct
    13261 acgcgccgct cgccaaccgg ctgggggtgt ggcagatcaa gtgggagctg gaagatctct
    13321 ccttccgctt catcgagccc gacacctaca agcgcatcgc gaagatgctc gacgagcgtc
    13381 gggtggagcg cgagcagttc atccgcgact ccatcgagcg cctgaagagc gagctcgccg
    13441 cggtcggaat cgaggccgag atcaccggcc gcgccaagca catctacagc atctacaaca
    13501 agatgcgcaa gaagcggctc gacttctcgc aggtcttcga catccgcgcg ctgcgcgtgc
    13561 tggtgcccga gatcaaggac tgctacacgg tgctgggtat cgtgcaccag atgtggcagc
    13621 cgatcgcgaa ggagttcgac gactacatca ccaagcccaa gggcaacaac taccagtcgc
    13681 tgcacaccgc cgtgctggcg ggcgacgggc gcgcgctcga ggtgcagatc cgcaccttcg
    13741 acatgcacaa gcacgccgag ctcggcgtgg ccgcgcactg gcgctacaag gaaggcgcca
    13801 ggcagggcgg cgactacgac gagaagatcg cgctgctgcg cagcctgttg tcgtggcgcg
    13861 acgaggttac cgatgcggcg cactgggtgg agcagctcaa gcgcgcctcg ctcgacgaca
    13921 cgctctacgt gctgacgccg cagggcaggg tggtcgatct gccgcgcggc gccacgccgg
    13981 tcgacttcgc ctaccgcctg cacaccgacg tcggccacca ctgccgcggc gccaaggtcg
    14041 acggccacct catacccctc aacacccagc tggagagcgg gcagacggtg gagatcgtca
    14101 ccgccaagga gggcggcccc tcgcgcgact ggctggacac gcgccagggc tacgtcgcca
    14161 cctcgcgtgc gcgcagcaag atcaagcagt acttcgcaca actggacgag gaggagatcc
    14221 tctcgcgcgg gcgcagcttc gtcaccaagg agatgcagcg cgacggccac ggccaggcca
    14281 acatcgacgg cctggccgag cgcctcggct tcaagaacgc cgaggcgctg tacctggccg
    14341 ccgggcgtgg cgaggtgggc ccgcgcgcga tgcagaccgc gctgcgcgag ggcagcgccc
    14401 ccgagccggc ggcggaggcc gaacccgagt tcgtggtcag ccgcagccgc tcgggcgaca
    14461 atcaggacaa gatcctgatc gtcggcgtgg gcaagctgat gacctcgctg tcgcgctgct
    14521 gcaagcccgc gccgccggat gcgatcgagg gcttcgtgac gcgcggccgc ggcatctcga
    14581 tccaccgcgt cgactgcgcc gacttccagg acctcgcgcg caagcacccc gagcgcgtga
    14641 tcccggccga gtggggcgag aaggcgcacg acacccgcaa cgcgctctac caggtcgaca
    14701 tcaacgtcca ggcgagcgac cgccagggac tgctgcgcga catctccgag gtgctgtcgc
    14761 gcgagaagct caacgtcatc gcggtcaaca cgctcaccaa gaaaggcacc gcctacatgc
    14821 gcttcaccat ggaggtgggc ggcatcaagc aggtgcagcg cgcgatcacg ctggtgcgcg
    14881 aggtgcccgg cgtgatcgac gctcagcgca agtgagcgcc gcccccggcc ctgcggcggg
    14941 cttgcgcagt atcgggcttg gtccggcgag cgcttcagcg gcgccccccc atcgccgact
    15001 cgcgcagcac gccttcccgt atcgacgcct cagctgctcg ccgcgagctc cttcagcagg
    15061 atgagctgcg ccgcgcggcg gatgctgcgc gggtgcttgc ggcggtattt gccatctgcc
    15121 tgcatgtccc aggcctggca gttgtcgccg aggtaggggc gcaggccttc cttcatgacg
    15181 cggcgcttga ggcgcgggtc gagcaccggg aaggcgatct cgatgcggcg gaagaagttg
    15241 cggtccatcc agtccgcgct cgacaggtac atgtgctcgg cgccgtcggc gtggaagtgg
    15301 aagatgcggt ggtgctcgag gaagcggccg atcaccgagc gcacgcggat gttgtccgac
    15361 agcccgggca cgcccgggcg cagcgcgcac gggccgcgca cgatgaggtc gatctcgacc
    15421 ccggcctgcg aggcctcgta gagcgcctcg atggtctcgg gttcgagcag cgcgttcatc
    15481 ttcgcgatca cccgaccgcg ccggcccgcg cgcgcgatct cggcctcgcg ccggatcgcc
    15541 gcgaccacgt tgggctgcag ggtgaagggc gcctgccaca ggtggcgcag ctcggtggcc
    15601 ttgcccaggc cggtgagctg cttgaacacg gtggcgacgt cctcgccgat ctccgcgttg
    15661 caggtgagca ggccgaagtc ggtgtagaag cgtgtggtgc gcgggtggta gttgccggtg
    15721 cccatgtgca cataacggcg caggccgtct tcctcgcggc gcacgatcat cagcagcttg
    15781 gcgtgggtct tgtagccgaa cacgccatac acgacgtgcg caccgacctc ttccaggcgg
    15841 ttggccagcg tgatgttggc ctcctcgtcg aagcgcgcca tcagttcgag caccacggtg
    15901 acttccttgc ccttctgcgc ggcgcgggcg aggtgctcca tcagcaccga gtcggtgccg
    15961 gtgcggtaca ccgtcatctt gatcgccagc acctgcgggt cgtcagccgc cgcgcgcagc
    16021 atctcgatca ccggggcgaa actctggaag gggtggtgga gcaggatgtc gccgccgcgg
    16081 atcgcggtga agacctggcg gcgtttgtcg agcaccttgg gcaggccggg cacgaagggt
    16141 gcgtacttga ggtccgggcg ctcgacccag tcggggacct gcatgaggcg caccaggttc
    16201 acgatgccgg gcgtgcggta gaggtcgtcg ggggtgaggt ggaagtgctg caggagaaaa
    16261 cccgccatct cctcggagca gttgtcggcg acctccaggc gcaccgcgtc gccgaagtga
    16321 cgttgctgca gctcgccctt gagcgaggcg cgcagatcct tgacctcttc ctcgtcgacg
    16381 aacaggtcgg agttgcgcgt gacgcggaac tggtagcagc cgagcacgtt catgcccgag
    16441 aacagctcgc ccacgtgggc gtgcagcacc gaggacagga acacgaagcc gtactcctga
    16501 tcgcacagct cgcgcggcag gcggatcacc cgcggcagcg cgcgcggcgc ctgcacgatc
    16561 gccgcgcccg agtcgcgccc gaaggcgtcg cggccttcga gctcgacggc gaagttcaag
    16621 ctcttgttga gcacgcgtgg gaagggatgc gccgggtcca gcccgatcgg ggtcagcacc
    16681 ggcatgacct cgcgcatgaa gtagtcgtgg atccacgcgc gctgggcctc ggtccacagg
    16741 ctgcggcgca ggaagaccac gccttccttc tcgagtgccg ggaggatgtc gccgttgagc
    16801 agctggtact gctcggagat gatctcgtgc acctcgtcgc tgacgcgctc gagcagctcg
    16861 acgggcgcga tcccgtcctc gcccgacttg cggctgccga tgcggatctg ctccttgatg
    16921 ccggagacgc ggatctcgaa gaactcgtcc aggttgctcg acacgatgca caggaagcgc
    16981 aggcgctcga gcagcggcac ggtggggtcg gcggcctgcg ccagcacgcg gcgctggaat
    17041 tgcagcagcg aaagctcgcg gttgaggaag tgctcgggcg ggtagttcag ggtcatgcgg
    17101 tcgacgggca ttcgggattc ctgtgcgcgg aagttcgctc gcgattatgt gacatttcca
    17161 tttcggtttg atgacgtcgg gccgcgcccg cggggccttc cccggcgcca tgccggcgcg
    17221 gctcggaagc cttttgcggg gtgctagaat ccggggttcc ggcgccccgc gggtgccagc
    17281 cgccgacaaa accatgcaag atctgatcgc cgcaatcgac ctcggttcca acagcttccg
    17341 gcttcaggtg ggccgcatcg tcaacgacca gatctatccg ctcgacggtc tcaaggaggc
    17401 ggtgcgcctg gccgccggcc tgtcgcccga caagatgctc gacctcgcct cgcagcagcg
    17461 cggggtcggc gccctgcagc gcttccacga gcgcctgcgc gacttcccgc ccacccgggt
    17521 gcgcgccgtg gcgaccaaca ccttgcgcgt ggccaagaac gcgcccgagt tcctgatccg
    17581 cgccgaggcc gcgctcggtt tcccgatcga gatcatcgcc ggccgcgagg aggcccgcct
    17641 gatctacgtc ggcgtcgcac acacgctgcc ggatccgcac cgccagcagc tggtggtcga
    17701 catcggcggc ggttcgaccg aattcatcat cggcaagagc ttcgagtcgc agctgctgga
    17761 gtcgcgctac atgggctgcg tcggctacag cctgcgctac tttccggagg gccgcatcga
    17821 caagcgcgcc ttcaaggagg ccgaactcgc cgcccgccgc gagctgcaga cgatctcggc
    17881 ggcctaccgc gaggccggct gggaagaagc ggtgggttcg agcggcactg ccaaggcgat
    17941 cgtggagatc ctcgagcaga acggcctgtc ggcgagcggg atcacccgcg agggcctgga
    18001 gaagctcaag gcgctgctgc tgcgcgccgg cagcctggac aacatcgacc tcgccggcct
    18061 caagggcgac cgcctgcccg tcatcctggg cggcttcgcg atcatgagcg cgatcttcaa
    18121 ggagttcgag ctcgagcaca tggtgttctc cgaaggtgcg ttgcgcctgg gcgtgctcta
    18181 cgacctgctc ggtcgctacc accaccacga tctgcgcgac gccacggtaa gcgccttcgt
    18241 gcggcgctac ggcgtcgacc tgcgccaggc ccagcaggtc gccgacacgg cctgccacct
    18301 gctcgcccag ctcgagccgg agaccgccga tcccgagcac gacgaccacc gcttcctgcg
    18361 ctgggcggcg atgctgcacg agatcggcat ctcggtggcg cactcgagct accacaagca
    18421 cagcgcctac attgtcggca acgccgacat gcccggtttc tcgcgcatgg accaggcccg
    18481 cctggcgcgc ctggtgctgg cgcatcgtgg caagctcgaa cgtgtcgcct cgatcgaccc
    18541 tggcagcgtg gactggctgc tgatcgcctg catgcgcctg gcggtggtga tccaccgcgc
    18601 ccgcgacgcg cgcggcctgc cgcccgtcac gctgcgtcgc gagggccgcg gcttcagcct
    18661 caccgccgcg ccgggctggc tgcagaagct gccgctcacc gcggccgcgc tcgacgagga
    18721 gcagcgccag tggcagggtc tcgggcgcgc gctctacatc cgggcgctca gcctgcgcac
    18781 gcctgtggcc tgagcgcaga tggcagggcg tgtgctcgac gaggtcttca cggccggagc
    18841 cgagggcatc cgcctcgccg gcgactggac cctgcgcggt ctcgacgagt gcctcccgac
    18901 gctgcgccgg gcgctggcca cggtgcctcc cgacgcgcgc tggtcgctgc gcgcggtgcg
    18961 ccgcatggac agcttcggcg ccttgctgct gtggcaggcc tggggcggac gctggcctgc
    19021 ggcgctcgag gccgatgacg cccagcgcgc catcatcggc cgtatcgaga acgcctccgc
    19081 ccgtgcgctg cccgagccgc cgcgcgcttc cgcgctcgac gcggtggcgc tgctcggcaa
    19141 gggcctgttc gcattcgctg gccatctttc cggcttcgtc gcgctcctcg gtcgtctcgt
    19201 gctcgatctc ggccacctgc tgcaccatcc acgcgactgg ccgctgctgg agatctcggc
    19261 aaacctccac aaggtaggcg tcaaggcgat gccggtctcg gccctggtcg gcttcctgat
    19321 cggtgtggtg ctgtcctatc tgtccgcgct gcagctgcgc accttcggcg cggacgcctt
    19381 catcgtcaac atcctcggca tgggcatcat ccgcgagctc ggccccgtgc tggtgtcggt
    19441 gctggtggcg gggcgctccg gttcggcgat gacggcgcag ctcggggtga tgcgggtgac
    19501 cgaggagatc gatgcgctcg cggcgatggg cgtctcgcgc agcctgcgcc tggtgctgcc
    19561 caaggtggtg gcgctttccc tcgcgatgcc gctgctcgtg ttgtggacct cggcgatcgc
    19621 ccttttcggc ggctgggtgt cggcctgggt ggagctcggc atcgggctgc agttcttcct
    19681 gcgggcgctg cccgatgcgg tgccggtggc caacctctac atcgggctcg ccaagggggc
    19741 ggtgttcggt ttcctgatcg cgctcgtggc ctgccatttc ggcttgcggg tgaagcccga
    19801 caccgagagc ctgtccgcga acaccaccgc ctcggtggtg gcggcgatca cggtggtgat
    19861 cctggtggac gcggtgttcg cgatcgccac ccgttcgatc gggctgccgc tgtgagcgag
    19921 cgggcgacga gcgacgccgt ggtctgcctc gaaggcatcg tcacccgttt cgggcgcaat
    19981 atcgtgcacg aggggctgga cctcaccatc cgcgcgggcg acatcgtcgc cctcgtgggc
    20041 ggctcgggca gtggcaagac cacactgctg cgccacctcg tcgggctgag ccggcccgcc
    20101 gccggccggg tggaggtgtt cggcgaggcg ctcgatgccg gcggggcggc cgcgcggctc
    20161 gcccgccagc gccgcatggg ggtgttgttc cagcacggcg ccttgttctc ggcgttcagc
    20221 gtggcgcaga acatcgcctt tccgctgcgc gagctcggcg tgatcagccg gccggagatc
    20281 gaccgcctgg tcgccctcaa gctggcgatg gtggagatgg agccggcgca tgcgccactg
    20341 atgcctgccg agctgtccgg cgggatggtc aagcgggtgg cgctcgcgcg cgcactcgcg
    20401 ctcgagcccg agttgctgct gctcgacgag cccaccgccg ggctcgaccc cgaccgcagc
    20461 gcgagcttca tccgcctgat ccgcgcgctg catcgcgagc tcggactcac cgtggtgctg
    20521 gtcacgcacg atctcgatac actggcgcag atggccacga gcgtggcggt gctcgccgag
    20581 cgccgcatcg tcagccacgc ctcgctcgcc gacacgctcg cctacgacca ccccttcgtc
    20641 gcccgctttt tcagtgggct gcaggcgcgc tgcggccagc cgcgcggcgc aggagcaggc
    20701 tgaaatggaa aaccgtgccc acgcgcttgc cgccgggctg ttcgcaatct tgctcggtgc
    20761 cggcgtcgtg ttcgcgatct ggtggttttc gggccagcgc gtgccgatgc gcgagatcgt
    20821 gctcgaggca cgcggcgaca tcaacggcct gggcgcacag tcgcgcgtgc gctaccgcgg
    20881 catggcggtg gggagcgtgc gcgccgtcgc gatcgacccc gaggacctgc gcacgctgct
    20941 ggtgcgcatc gcggtgcccg ccgatctgcc cctgacccgc ggcaccaccg ccgcgctcgg
    21001 cacgctgggc gtgaccggcc tggccttcgt gcagctcgac gatcgcgggg tggaccgccg
    21061 gcccttggct gccgccgatg gcgaggtgcc gcgcatcgcc ttgcaacccg ggctggtgga
    21121 ggcgctctcc gggcgggcgc tcgccgccct cgaccagttc caggcgctcg gcgagcgcct
    21181 gcagggggcg ctggccagcc tggagtcagc ggccgcgggc gtcgatcgcg gggtggcgga
    21241 actgccggcg accctggccg cgctgcgggc cgccttgagt gccgggaaca tcgcgcgcat
    21301 cacctccgtg ctcgggaacc tcgagcgcgg ctcggccgaa gccgtgcccg ccgttgccga
    21361 gctgcgccgc ctgatcgtcc gcatcgatca ggcggccgcg cgtctggagc tccgcgccgc
    21421 cgccgcaggg gacgacctcg tcgagcgcac gctgccgcag ctcgatgtcc tgctcggtga
    21481 gctcacgagc acctcgcaac gcttcggcca cctggtcgag gagctcgacg cctcgccgca
    21541 gctcctgctg accgggcgcg accgtcctct gcccgggccc ggtgaggcgg gccacgaggg
    21601 ctttgcgggg agcgaccgat gagccgaagg gagaaaggaa tgcagccacg tcatcccgac
    21661 tgccaccgcg tcctgccgcg cctgtccgcg tgtatcgcga ccgcgctgct ggtggccgcc
    21721 tgccaggtgc tccccgagcg tccgcgtgcg gcgacgacct acgatctcgg caccggcgag
    21781 cgcgccgcgc tcgcccacgg gctcgcgccc gcgcgggtgg cggtgtcctc gcccccatgg
    21841 ctgcagacga cgacgatgca ttatcgcgat gcccgggtcg atccagggcg tcgccgcgcc
    21901 tatgccgaca accgctgggc cgctccacct cccgccatgc tcggactcgt gctggagcgc
    21961 acgctcggtg ccggcgaggg gggcgggtgc cggctgcagg tccagcttga cgagttcgag
    22021 caggtcttca ccgcgggcga acgcagcgag gctcgtctcc tggctcgctt cgccctgctg
    22081 ccggtgcgcg gcgccgcggt catcgccagc gcttcgctcg tcgtggcgga gccggccacg
    22141 acggccgatg cgagcggtgg ggcgagcgcc ttccgtgcgg cgaccgagcg gctggcgctt
    22201 gccatcgcgc agtggatcgc cgagaccgac cgcgcgctct ccggcgggct cggcatcgcg
    22261 ggtcgctgcc agcgctgagg aaggcgatgc cgcggggggc ggccgtctcg agcgcaccgg
    22321 cggcggcctg gtggagtggg gcgctcggtt tcagcgggcg gcgatcacga cctggttgcg
    22381 cccggcgtcc ttggcacgat agagcgcatc gtcggcgtgc tgcagcacca cgccgagggt
    22441 ctcgcccggt tccgccaggc aggcgccgat ggtgaccgtg acgcgttcgc cgcaggccgc
    22501 gctgccgtgc tcgatgtcgg cgcggacgcg gtgggcgatc gcctccagcg tggccgcgtc
    22561 gcaaggcgag agcagcacga ggaattcgtc gccaccccaa cgtgcggcgc ggtcgtaggg
    22621 gcgcaggcat ccttccagga cacgcgccag cctcgtcagc gcctcgtcgc cggcaccatg
    22681 gccgcggcgg tcgttgatct ccttgaagtg atcgacatcc agccacagca ggccgaagcg
    22741 gctgccctgg cgttccgcgc ggatgatctc ggcctccagc atttcgctca tgccgcggcg
    22801 gttggtcagg ttggtgagcg gatcgatccg cgccagccgg tgcagggcgt cggtgcgctc
    22861 gcgcaccttg gcctcgagct ccgcggtgtg cgcccggatc gcgcccgcca tcgcctcgaa
    22921 gtgctgggtg agacgcccga tctctccact cgcctgcgcc agcggcgcgg ggctgaggtc
    22981 gccgtcgcgc acccgcagca tggcctgctc cagcctgccc agagggtcga gaatgaggcg
    23041 acgcaggacc agatggacga ggacgagcgc gaccagcagg gtgaggacga aggcgactgc
    23101 gaccggggtg agggctgcca ccggcatcag tacgtcgagg tcgagcaggg tgatctcgta
    23161 ccagccgatc tcgggcacga aggcgattcc gagcagggtc cgccgcccgc cggactcgac
    23221 aaagcggtcc aggacccgcg gaacgccgtc gcccgacgcc gccagggcac tcatcgtctc
    23281 gcgcagtgcc acgcggtcgg actcgcgatc gagcagctgg tccgccaggc tcttctgccc
    23341 ttccggcttg accaggctgg cgaagtcgat gagcttcgcg tcgcggtgaa gctggatcgc
    23401 accggaaaga tctgcgaaca gcgtgctcac gccgtcctgg tgcaggtcca cgatctcgtc
    23461 cagaaagcgt gtcagctcga tgccggtgcc gaccacgccg aggatctcgt cgccgtcgcg
    23521 gatgaggacg tcgatccaca gcttggtgac gcccagttcg gcatcgggat tgacgttgag
    23581 gtggaagtcg ggcccgacct ggacgagctt gtagaaccac gcgtcggcgg gtttggcggg
    23641 gtcgaggtgg tagcgcagct ggcggccggc gaactcgtcc ccggcgttgt tgtggtaata
    23701 ggccccggag gtgcgcagcg cgacgaaata gttgccggag ctgaagttgc ggcggaagtt
    23761 ctccatttcg cgcactgcct cggcttcgag cgccggatcg acgggcgagc gtgcccagcg
    23821 tttgagcacg agcgagtcgg ccatctgccg cgccagcgcg atctcgcgtt cgagcggttg
    23881 caggaggcgg gcgcggtcgt agcgcacctg gatgtcggcg acgcgttgtc cccactgcat
    23941 gacgatctgc cgggcaagat gatggaacac cgcccatgcg gataccgagc cgaccacgat
    24001 gagcgcggag atgaggagga gaaatcgagt tctcaggttc acgcgccggc actccctgcg
    24061 ttcgggtcgc tgtccgtggc ttcttgctca gtttgggctg ccggcgcggg cgcaggtgtg
    24121 cgttttctca cacccggacg catggcgcga cgaatcgtga tgggcggagc gctgcgcgaa
    24181 gcgctggcgt ggcgcgcttg ttgacgttaa cgttgccgcc cgtggccaca tggccctgcg
    24241 aacacgaaac cacgaggaga gaaccatgaa cgccgaattc cgccccgatg ccgcagccga
    24301 agcccccatc ctgcgccgcg aggatgccgg cggcgtgacc accctcacgc tgaaccgtcc
    24361 gcagcagttc aacgcgctct cgttcgagat gctcgaggcg ctgatcgccg cggtggatga
    24421 gatcgcacgc gacgagacgg tgcgcgtggt ggtcatcgcc ggcgaaggca aggccttctg
    24481 cgccggccac gacctcaagg agatgcgcgg caaccatacg ctggaattcc agcagcgcct
    24541 gttccgcctg tgcggcaggt tcatgatgaa gctgaccgag ctgccccagc cggtgatcgc
    24601 gcgggtgcac agcatcgcca ccgccgcggg ctgccagctg gtgtcgatgt gcgatctcgc
    24661 agtggcggcc gagcacgcgc gcttcgcggt gtcgggcatc aatgtcggcc tgttctgcgc
    24721 cacgcccggc gtcggcctgt cgcgcaacat ggggcgcaag gaggccttcg agatgctggt
    24781 gacgggcgat ttcatcgacg cccgcgaggc gcagcggcgc gggctgatca accgcgtggt
    24841 gccggccgac cagctcgacg aggaggtcgg ccgcctggcc gcgtccatcc tggccaaatc
    24901 gccggtggcg atccgcatgg gcaagcagat gttctacaag cagctcgaga tgggcctcga
    24961 cgccgcctac cagctcgcct ccgagaccat ggcctgcaac gcgatgtgcg aggacgcggc
    25021 cgagggcatc gatgccttca tcgccaagcg caaacctgag ttcaaggggc gctgagcggc
    25081 gtcgggatca tccccgtcga gcaatgcgaa ggcgaccggt gctgatcgac acccacgtcc
    25141 acctcgatgc tgcggagttc gacgacgacc gcgagcaggt catcgcgcgt gcgcgcgccg
    25201 cgggggtagg gcgcttcgtc gttcctgcag tcgagcgccg ggggttcgat gcggtcgagc
    25261 ggctcgccga cgaccatcgc ggcatcgttc atgcgctcgg catccacccg ctctacacga
    25321 tgcgggcggc gcgggaagac ctcgaactcc tcgaccagcg cctgaaccgt ggcagggcga
    25381 tcgccgtcgg agagatcggc ctcgaccatt acgtgaccga cgtcgatcgc ggcgtacagc
    25441 tcgagttctt caccgcacaa ctccgcctgg ccgctcgcca cggactgccg gtgatcctgc
    25501 acgtgcggcg tgcgatcgat ccgatactcg cgcaactgcg ccgcatcccg gtcccgggtg
    25561 gaatcgcaca tgcgttcaac ggcagccgcc agcaggcgga catcctgatc gggatggggt
    25621 tcaagctggg ttttggcgga tcgatgagct acggcggatc cacccggatc cgcgagctcg
    25681 ccgcctcgct gcccttggag gcgatcgtcc tcgagaccga tgcacccgac atcgcgccgg
    25741 aatgggcgcg cggcaggcgc aatgagccgg cgaacctcgc ccgttacgcc ggtatcctgg
    25801 cggagctgcg agggatggcg cccgaagagg tggcggacgt gaccacggcc aacgccatcc
    25861 aggtcctgca ctggccaccc ctcgaagaag aataggcgac gcgggcgccg agatccccgg
    25921 cgcccgcttg tcgatttttt ttacattttt ttcgcgagcc actcgatcga ctcggcaagt
    25981 gtctgtattg atggggaaga gcttcctggt tcgaaatttg ctttagccga accgtcgcgg
    26041 tgatgaatgc atacccttgt gccgcgaggt ttcgagttaa cgcttattgg acgggcctcg
    26101 tacgcccgca accgggagaa aacaacatga aaaacggaat ggccctgctc gccgccatcg
    26161 gcatcgccac cctttcagcg agcgcgcaag ctgcgctcat ccgcctcgag gtttcgggca
    26221 atctcgccta cgggaccgat cggaccaacg tcttcggcct gggggcaaac accagcctca
    26281 ccggacgcgc cgcgaccttc tcgtggctca tggactctgc ttcgctggga tccgatcgca
    26341 acaccgatcc gaccctctcc gcctacggat gcggatccgc agcccccggc tgcacctact
    26401 tcatgagcgc atcggtcacc atcaacgggg tcacgcgcac ccttgcaggg cgggggagcg
    26461 acctcacgaa cgacgtgcgg atgtacgcgg acaccaatgg ccccggtctc ggctcggcgg
    26521 acggctacta cctgcggatg gggcagacgc gggatgtcgg aaactggtat ctggacgagg
    26581 gcggcaactg gtatcgggcc gcgacgcgga tcacgtccaa ggtggagatc ggcgtaaacg
    26641 atgcagtcga ctccatcctc gccggcctcg atccggcgga gctccacggc tggtccacca
    26701 gcctgctgac gggcacgaac gatgacggga gcacggtctt cgtcttcagc gagaccgatc
    26761 tcaacggcgt gcagattttc gagtacgcaa ctggtgggct gttgttgacg gaccgctcca
    26821 cgcggtggtg gaccgacact gcgcccagtt cgggttcggg caccgggggg accaacaacc
    26881 cgggcggtgg cacgaacgct cccgcggtaa cggtgccgga gcccgcaacg atcgccctgc
    26941 ttggactggg gctcggattg ttgcgcagga aatccaggcg ccattccggt cgagcctgat
    27001 tccgccctcg acgcctgcgc gcgcgcatcg ctcggcgcgt gcgctgccgc gcaggcgcta
    27061 agcgaccacc ctcgacggcg agcgcgcgat caggtcggcg atgtcgcgct tgagccggac
    27121 gacgtcgcga tcgaatttct cgtagatcat cagcgagacc ccgaacacgt aagcgtacaa
    27181 cagcatgcag cggctggaag cctcgtccat cggcaccccg caggcgagaa agaggtcgcg
    27241 tgcgcagcgc aggcggacgt cgtcgacctc ggcgacgatc gccgcggcct cggcgtcccg
    27301 acgcgcccag tcgcgcacgg caagctcgat catcatgccg cgacggctgc ggctcgcgct
    27361 gtagacgtcg atgacgtggt agatctgctc gagctcgcgg ccaggctgtg cccgcgtctg
    27421 cttgatgatg tcgcggattc gaccttcctt ccactgcgcg aggacggcca tcagcaggtc
    27481 gcggcggtcg gtgaaatgcc agtagaaact gcccttggtg accttgagcc gcttcgccag
    27541 cacctccacg cgcaagcccg cgatgccttc ctcggcgagc acctcggtgg cgcctgcgat
    27601 ccaggcggca cggtcgagct gggtacgcgg ctttttcaca atgttttcca tacggcgtag
    27661 tattgacgat tcggtgacca tacgctaccg tatgcgccat acttggccgt atggaaatgc
    27721 tgctgcgcag cagtgcgacc ggccaagggc ggtgacataa tccgaaagtc gggtccgcat
    27781 ttcgggccta ccatcccctg caaagaagag gagcgcgtca gagtgaagat tctcgtaccc
    27841 gtaaagcgcg tggtggatta caacgtcaag gttcgcgtga aggccg
//
LOCUS       DAOQBS010000029        27045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1106_length_27045_cov_15.018970, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000029 DAOQBS010000000
VERSION     DAOQBS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 27045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27045
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1106_length_27045_cov_15.018970"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1068)
                     /gene="alc"
                     /locus_tag="PKN25_05335"
     CDS             complement(19..1068)
                     /gene="alc"
                     /locus_tag="PKN25_05335"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HNV90357.1"
                     /translation="MASHTPGAPVFAPAAELPDWALRSVDLANPRLGAKAIAASDDFF
                     AEVGRMLNPEPAQFVPGKFDTNGKWMDGWESRRKRVAGHDWALVKLGVKGVIRGFDVD
                     TSHFTGNYPPAVSIEACVSETDDVAALQAAEWTEILPASPMGPNSHHLLECASTAAWT
                     HLRVNMYPDGGIARLRVYGRPVGTLAAKAAGGELVDLVAMENGGRALSWNDASFGSSA
                     AALLLPGRGMNMGDGWETRRRREPGNDWCVLELGAPGTVERIEVDTAFFKGNYPDRCS
                     VQAAYVSGGTDRSITTQSMFWQTLLPEQKLEMDAIHTFTEQVAKLGPITHVRFNIFPD
                     GGVSRLRLWGKVEAK"
     gene            complement(1170..2102)
                     /gene="puuE"
                     /locus_tag="PKN25_05340"
     CDS             complement(1170..2102)
                     /gene="puuE"
                     /locus_tag="PKN25_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HNV90358.1"
                     /translation="MTSNPTYPRDLVGYGRNPPHADWPGRARIAVQFVLNYEEGGEHC
                     VLHGDAGSEQFLSELFNAASYPDRHLSMEGIYEYGSRVGVWRFLREFERRGLPLTIFG
                     VSMALERHPELTAAFKELGHEIACHGWRWIHYQNVPEEIEREHMKIGMEIIERLTGTR
                     ALGWYTGRDSPNTRRLVADHGGFLYDSDYYGDDLPLWTEVQKTNGETVPHLVVPYTLD
                     TNDMRFALPQGFSHGDEFFEYLRDAFDVMYAEGEERPAMMSIGMHCRLLGRPGRFRAL
                     QRFLDHIEKHDRVWVCRRVDVARHWIERHPYQAK"
     assembly_gap    2130..2229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2280..2852)
                     /gene="uraD"
                     /locus_tag="PKN25_05345"
     CDS             complement(2280..2852)
                     /gene="uraD"
                     /locus_tag="PKN25_05345"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HNV90359.1"
                     /translation="MSTPATAPTTAPDRPAAALDAALDELSRLAQDAFVARLEGIFEH
                     SPWIAERAWSAGPFASIDALHAAMCLVVDTAAPAEQLGLICAHPELAGKEAEEGTLTT
                     ASTGEQRGAGLDQCSKEELQRLRRLNQDYRARFGFPFVVAVKGLTRHQILDRVEARLA
                     NARDAEFRTCLAEIGKIARFRLDAMFGTNS"
     gene            complement(2882..4075)
                     /locus_tag="PKN25_05350"
     CDS             complement(2882..4075)
                     /locus_tag="PKN25_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HNV90360.1"
                     /translation="MEAYLLDWANLLVRWLHLIAGIAWIGASFYFVMLDNSLKPPKNP
                     NDAKRGVFGELWAVHGGGFYHSQKYLTGPKGEPLTQDLHWSKWEAYTTWLSGMGMMAV
                     IYWIGASSYLIDSSVMALSPAAAVGISIAFLVAGWVFYDLLCRNMIGKDGVLAAIVFV
                     FVMVANYVLHQVFSARGAYIHVGAMLGTMMAANVFFHIIPGQKKMVEQIRRGEQVDPM
                     PGIVGKQRSVHNTYFTLPVLFIMISNHYPMTYANAKGWLVLGVLMLAGVLIRQFFVLR
                     HRGQVKWWLPAAGVALIALLMVLMAPKQVDAGGEKVSFATVKAIMAQRCIACHADKPT
                     FEGFAQAPKGVMLQTPEQIAAHAVMSAQTVASGYMPLGNLTAITEEEREKIAIWFAQG
                     ARTAD"
     gene            complement(4129..4479)
                     /gene="uraH"
                     /locus_tag="PKN25_05355"
     CDS             complement(4129..4479)
                     /gene="uraH"
                     /locus_tag="PKN25_05355"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HNV90361.1"
                     /translation="MGRLTTHVLDTAHGKPGTGIAVTVYRLDGERREVARSVTNHDGR
                     CDKPLLEGAALEAGKYEIVFAAGDYFRALGLNLPEPPFVDEVALRFGIADVAEHYHVP
                     LLVSPWSYSTYRGS"
     gene            4658..5596
                     /locus_tag="PKN25_05360"
     CDS             4658..5596
                     /locus_tag="PKN25_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV90362.1"
                     /translation="MSLRRSDDPLDTYLLRVFVLLISERSVSRTALRLNQSQPAVSAA
                     LKRLREILGDPLLVREKGGMVPTERALALMSHAKGALAEIDRMTDAPESFEPHSTRHE
                     FRVGSPDYLAPVFVASVAERFRREAPQARLSLHALGPSFDFERSLAEGDLDVVIGNWP
                     EPPHRMHLSMLLEDEIVCLVAASHPCAKKGMSVDDYLRAAHVVPMPYSISQRGVIDSV
                     LASMRISRDERVAVQSFTAAPYLLQGTDLVFTTTRHFAQFYANLLPLAIIPSPIAFPP
                     MRFYQLWHERNHHSTGHRWLRRLLSDCGRRIAPPHA"
     assembly_gap    5671..5770
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5781..6254)
                     /locus_tag="PKN25_05365"
     CDS             complement(5781..6254)
                     /locus_tag="PKN25_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV90363.1"
                     /translation="MSYEIRSILYASDLTPRSPAVFRHAVGLAMKFNAKLHAVTVTLP
                     SSSLPYEEFLSEEKMDEIKLAGHKKAEALLRHRIDVFAEANPELDVKSVLASVRALEG
                     DPSRRILDVAKHVLADVIVMGSRGHSTIGELLLGSVAHKVTMKADIPVMLVPIDR"
     gene            6531..7241
                     /locus_tag="PKN25_05370"
     CDS             6531..7241
                     /locus_tag="PKN25_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNV90364.1"
                     /translation="MIDLYTAATPNGHKVSIALEELGLAYSLRVLDLSANEQKEPWFL
                     AINPNGRIPAIVDHDEGDFAVFESGAILIYLAEKTGRLMPQDARGRSRVLQWLMFQMG
                     GIGPMMGQANVFFRYFPEKIQPAIDRYQGEVKRLFRVLDGHLAKNEYLAGDYSIADIA
                     NWAWVRTHNWSGVAIDDLPDLARWRDQIRARPAVQRGIEMPPSSFDRDGDGEEQAKAF
                     SAKVRNMVETGQSRSGGV"
     gene            7286..7906
                     /locus_tag="PKN25_05375"
     CDS             7286..7906
                     /locus_tag="PKN25_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNV90365.1"
                     /translation="MKLYVSPRAPNPRRVQMFIAEKGIRGIEEVAVDLVKGEHRSSDH
                     RARSPLAKVPVLELDDGRYLGETRAICSYLEGLYPEPNLMGESFEERAFVEMTDRRVE
                     LYLFGGIMNCVRHTHPGLAPLEQPQFEDFGRSQGEKVREVARWLDEELGRQPFVAGER
                     FTIADITAFCAIEFARGLMRFRPGAEGMANLQDWRDRIAERPSARV"
     gene            complement(7929..9719)
                     /locus_tag="PKN25_05380"
     CDS             complement(7929..9719)
                     /locus_tag="PKN25_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNV90366.1"
                     /translation="MITEMEIFPWDSNFATGIAVIDAQHQQLVALLNRLVTHLAFQSD
                     APTLNSVFDQLRDYVEVHFTTEEALWQAHLGEYAGVAGHHESHVRFIDEVLRLKAEES
                     TKPFETVIENIVNFLTRWLALHILEADRRMALVVHGVQRGLTLEQARTQADETMSGSM
                     RVLVETVLVMYDKLANRTMAMTREINRRRQAEAELQKAHEALKRAKENAESVSEAKSS
                     FLASMSHEIRTPLAAILGMSHLMHQEELPASQRERLDVIENAGRHLLSIINGILDLSK
                     IEAGKFTLDEAALDLRGVVEEAMAMLAHQAEAKGLSLRAEFDLPPGTFLGDATRLRQA
                     LVNYLINALKFTERGGITVRCSASAVDTDAVDVRFVVEDTGPGIAAAELRRLFKPFTQ
                     TEATSTGEHGGTGLGLAITARLAELMGGQAGAESEPGRGSRFWFTTRLRKVKADEVVA
                     PTGSPLEIRGALAERHGGACVLLADDDKVNREIARVLLSRGGLAPECAADGEEAAALA
                     ERCKPALILMDMQMPVVDGIAATRRIRTQDWGREVPIIALTANAFAEDRVRCLEAGMD
                     DFLTKPIMPAVLYSKLLYWLDRSRRERAGS"
     gene            complement(9859..11091)
                     /locus_tag="PKN25_05385"
     CDS             complement(9859..11091)
                     /locus_tag="PKN25_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV90367.1"
                     /translation="MNLETLKVLVVTDVAADAAQIARLLADHFEHIETSTIESRFIAD
                     FERVQPDVVVLAFTSLERAERYSLGLYRHSEVVHSLPHRSIVLCDKDNVHRAFELCRK
                     EYFDDYVLYWPMVFDTPRLAMSVLIAGRSLQAQRDTPNAREVAAHARSISQLETVLDD
                     RIEVGRQHADALERALQAAEIEASSVQPRPAAPAAPSSDSATIPDAGLDVLPAFTDSF
                     ELDVGDVPSGLDRPTQFAVTPQEDVIQEAKLRVDEAKKKVVPLTEWIGGLKADVKPQL
                     DAARQLGELAGRTPPLIVVVDDDAFQCKLLERLLGNSGYRSIIAHSGAEALAVLGRQP
                     PDLILMDVALPDFNGVELTRRLKAIPRFAAIPVVMITGHSERQVLEASLKAGAVDFLV
                     KPFDREILLQKLARHLAP"
     gene            complement(11130..14354)
                     /locus_tag="PKN25_05390"
     CDS             complement(11130..14354)
                     /locus_tag="PKN25_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV90368.1"
                     /translation="MNDPHPAQRATVSLPAPTPALPPSAAAIELFIEACPFAVIEWGR
                     DMRVLRWSPEAERVFGWSAAEVLGRRPNEWSFTHPDDAAAVKRAIGQAITADGPTPPV
                     IGRNFTRDGRLLHCEWHNRANRDPQGRLVSLLSFAKDLTRQLEAERARDLSEARYAHI
                     FNNSHAVMLILDPESGRILDANPAAEEFYGWSRETLQTMQIGDINTLSPQALLVELKA
                     AHAEERKHFEFRHRRADGSVRDVEVHSGPTEDGNRSMVFSIVHDITERKQAEALSRRW
                     ERFFRLSNLGLAMHDVSDNTIIDVNATYASQHGYSIEELRGMRIDELYPEDEREQLHA
                     HLAEADRIGNASFETVHLRKDGSRLPLVIGVTALLDDRGRAVARFAFGLDISARKAAE
                     DELRKLSRAVEESPESIVITNTRAEIEYVNQAFIDKTGYSRAEAIGQNPRLLQSGRTT
                     PATYADLWNTLTHGRSWQGEFFNRRKDGSEYLERVTITPIHDESGHITHYVAVKQDIT
                     AQRRMEEELLRYHEHLEGLVESRTAELQHALDAANIASRAKSEFLTTMSHEIRTPMNG
                     VIGLLDVLSHSQLSTEQVEMVGIMRESAETLLRLIDDILDFSRIESGNLELDVGPASI
                     PDLIARVVGILQTVANRKSVRLSTRIDPDVPAVVRTDALRLQQILCNLVGNAVKFSSG
                     LDRPGRVEIRVETAGAGRIRFMVTDNGIGIAPEAIEKIFDPFSQAESSTTRRFGGSGL
                     GLSICTRLVRMMKGRMEVNSLPGRGSRFVVTLPLAATNGSATRSEPARSTALARPSPT
                     PPLATPGAGEAGRRILVAEDNDINRRVIARQLALLGLQCDTAEDGFEALERWRQGHYS
                     LLLTDLHMPGMDGYELTARIRSEEAPGRRTPIVAVTANALRGEKERCIDAGMDDFILK
                     PVQVAALQEVLARWLQPDTEQRTPAGTAPAAPAAPPAVFDPQALPGLIGNDAALIAEF
                     LGEYRLSACDTVRSIRKACEDGDWRRAGELAHRLKSSSRSVGAMQLGEICAALEQAGR
                     DDDGDQVQRQGGLLEAALAATLTAMQGTQAAGVPPARGDTPRPG"
     gene            complement(14351..16666)
                     /locus_tag="PKN25_05395"
     CDS             complement(14351..16666)
                     /locus_tag="PKN25_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV90369.1"
                     /translation="MILDLLQATALLLALCLLQGGLARRCAHGGPAFAVGSGLIYGLA
                     CIAGMMMPITLAPGVIVDPRSVVLSVGALFGGPLAGAVAGLAAAAYRIWLGGSGVFIG
                     VTTITLAVLLGLAFRLGAARGWLRPAPLQLFLFGVFTHATSLLLIATVPNQSLAGLFA
                     NLGLPYLLLMGAATLLLGALLRDLEQRRATECALAASEARLRAITAAIPDLVFVVDED
                     GRYLEVITRQENLLAAPPEQVIGRRSEDVLPAGPAAVLRAALEQALASPERTCARYAL
                     DTPAGHREFQAWIQAIDAPIGGRRAAAILVQDITPNVAAEERIRKLAYYDPLTTLPNR
                     RLLVERLGHAIATNARTGLHGALMFIDLDDFKDINDLLGHHNGDVLLREAGARLRGVV
                     RESDTVARFGGDEFVVLLELLAESADEALLAARQVADKLLEQLGHPYRLDGLDRKCTA
                     SLGLVIFNGHEHTADELMQRADLSMYEAKRSGKNTARVFDPHMRTELADRLALETELR
                     GAIGTGAFLLHLQPQLDAGGRLIGAEALLRWNREGHGPTPPALFIPIAEQAGLMPALG
                     REAMALACEELARWVALPGADGLVMAVNVSAAQLYEPGFVEEVLALLARTGAPAERLK
                     LELTESLLLTDIERAIEAMTRLREMGIRFSIDDFGTGYSSLGYLQRLPLDELKIDRTF
                     VRDLPANASSLAIVRTIMALADTLRIRVVAEGVETSAQHACLLDNGCDAFQGFLFARP
                     EAVESFRARMSATDALHGSGAQPRGGADRDR"
     gene            complement(16828..17760)
                     /locus_tag="PKN25_05400"
     CDS             complement(16828..17760)
                     /locus_tag="PKN25_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV90370.1"
                     /translation="MDASLFTSMLFATVVAGTPLIIVALGLLVNEKAGVLNLGAEGMM
                     TMGAVAAFAVTHESGNPWLGVAAGMGAGMAMAALFGLLVLTLMANQVASGLALAIFGV
                     GLAAFVGKPYEAVALQAVPPIRIPFIADLPLIGEALYNQQALVYFSWALFWGLLWFLH
                     RSRAGLVLRAVGESPASAHAIGYPVIRIRYLAVLFGGAMAGIGGAFLSVFYTPMWAEG
                     MVAGRGWIALALVVFATWRPLRVMVGAYLFGGVMVTQLFIQGSGLQIDFPSQLLSSLP
                     YWATIVVLVAISRNGNTIRLNSPVSLGKPYRPEA"
     gene            complement(17771..18835)
                     /locus_tag="PKN25_05405"
     CDS             complement(17771..18835)
                     /locus_tag="PKN25_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV90371.1"
                     /translation="MLELEARAEPSRLMSWASPLLAVALMLVGGLAVFAALGKDPAEG
                     FRVFFLNPVKDLYALSELLLKATPLMLCAIGLAIGFRANVWNIGAEGQFMLGAVAATG
                     VALYFGDAESALVLPAMVLAGAAGGAAWAAIPALLRTRFNANEILVSLMLVYVAQLFV
                     SWLVFGPWKDPDGFNFPQTRMFAEAATLPMVIEGTRLHFGFAIALAALVAGYAFMNRS
                     HAGFRMRVAGEAADAARYAGFSATRMIWVGLLLGGAAAGLAGMGEVAGPMGQLTDKVG
                     SGYGFAAIIVAFVGRLNAFGILLASLLMALLYIGGEQAQQYLNLPNSISMVFQGMLLF
                     FLLGADVFIHYRLRLRRRGT"
     gene            complement(18828..20423)
                     /locus_tag="PKN25_05410"
     CDS             complement(18828..20423)
                     /locus_tag="PKN25_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90372.1"
                     /translation="MPIPYPPLQPDTAAGPARLVLQGISKAYPTVVANHDIGLVVRAG
                     EIRAILGENGAGKSTLMKIIYGVTRPSSGRMFWEGEEVAVASPAHARSLGIGMVFQHF
                     SLFETLTVVENVALALPGKPALDALARRIVEVSERYGLPVDPRRHIHALSVGERQRVE
                     IIRCLLQDPRLLIMDEPTSVLTPQAVRKLFETLRRLADEGCSILYISHKLDEIRTLCH
                     TATVLRGGKVTGECIPAQETPASMARLMIGKELPRCEHDARQAGSEVRLRLAGLSHAA
                     DDPFGTDLRDLHLDVHAGEIVGIAGVSGNGQQELLRVISGEAPLAEKFPVQICGVEAG
                     RLSPGRRRRLGMAFVPEERLGRGAVPTMSLADNALLTAAHNQGMVAAGFLRFGRARAF
                     ARRCIDEFDVRCGGTAAAAKSLSGGNLQKFIVGREILQAPRLLVCAQPTWGVDVGAAA
                     FIRQRIIDLRNRGCAVLVISEELDELFEICDRIAVIAKGRLSPVRAARDTSIEDIGVW
                     MSGLWPGAEHTAGAPLATETPHA"
     gene            complement(20491..21267)
                     /locus_tag="PKN25_05415"
     CDS             complement(20491..21267)
                     /locus_tag="PKN25_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HNV90373.1"
                     /translation="MSTRAHPACPACGSTDCRRSRWRSHEEHAGHPGLHPWRCLACDQ
                     RFLAPDDALRPRSRWPLVVGILSALLLLGIGIASVRMLDLDESGDAPGETRSAAAEPS
                     APTDALDARFRAARAALLDTARGREVSASAVSQLREAAEGGHTGAMVLLGKLYRSGIG
                     MPQNYELAARWLNQAAHAGDPEGMVEFGRLHRSGIGVERDAVQAYVWFNRAAALLNMD
                     GVQERDSIAVKLDADELRRAQALSLEAAEEAARQAAERSR"
     gene            complement(21281..21520)
                     /locus_tag="PKN25_05420"
     CDS             complement(21281..21520)
                     /locus_tag="PKN25_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90374.1"
                     /translation="MPTCPRCDGTDCRESPWKSADERLAHPGERAWRCMSCVHRFHAP
                     EPRRLLRDNPIVAAVGGSTLLLTIAVIAILWFWKS"
     gene            complement(21707..24580)
                     /locus_tag="PKN25_05425"
     CDS             complement(21707..24580)
                     /locus_tag="PKN25_05425"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNV90375.1"
                     /translation="MSETTSASTQNTVKASAANLPMQEISGEVLVEKYAKGDEQTVAE
                     VRRRVARALAAVEAEDKRAHWEAKFLEAQEKGFVPAGRINSAAGTTLAATLINCFVQP
                     VGDSVTEAVDGRPGIYTALAQAAETMRRGGGVGYDFSSIRPKGALVKGTASNASGPVS
                     YMRVFDRSCETVESAGARRGAQMGVLRCDHPDIEEFIHAKDEGDLTNFNISVGVTDPF
                     MQAVEADGDVELVHKAEPTEEVKAAGAYQRDDGMWVYRKVRARELWDQVMRSTYDHAE
                     PGILFLDRMNADNNLYYCETIEATNPCAEQPLPPYGCCCLGSINLTLFVKNPFGDKAE
                     FDYAGFGKVVDVSIRMLDNVLEVTHWPLAQQHAEAQSKRRVGLGFTGLGDALIMLGQR
                     YDTAEAREEARKISEFMRNRAYLASSELARERGAFPLFNADLYLSGGNFASRLPADVK
                     DKIRKHGIRNSHLLSIAPTGTISLAFADNASNGIEPPFSYTYTRRKRMADGTFKEYAV
                     EDYAWRLYKHLGGNVDKLPQSFVTALEISAQAHKDMVAAVAPYVDTSISKTVNVPEDY
                     PYAEFEDLYLTAWKAGLKGLATYRPNSVLGSVLSVTPTAEQKQPHDVEIADANKRLSI
                     KNLPAPVLSSLRWPGRPDLPDGNLAWTYMLNTPTGGFALFVGQVGNNGGSFPFEVWVN
                     GADQPRGLGAVAKTLSMDMRANDRGWLKLKLDTLARTVGERSFEMPFPPHGEKKLVPG
                     VVSAFAQVVNYRCEKLGVFDKEDESNGVLDTLFSLEEPKTGTDGTLSWTVDIYNPATG
                     EDFVLGLKEITLPDGVTRPYSVWLSGNYPRALDGLTRILSLDMRVMDPAWIGMKLRKL
                     LDYPEPLGDFMAFVPGTRRQQNWPSTVAYLAQLIIHRYAMLGVLDERGYPTREMGILE
                     SPRDDNEPKLMQGALCNECGNHTVIRKDGCDFCTACGAVGTCG"
     gene            complement(24796..25644)
                     /locus_tag="PKN25_05430"
     CDS             complement(24796..25644)
                     /locus_tag="PKN25_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV90376.1"
                     /translation="MHSAQELVSQIEALTSLPDVYMRVREQLESPAGSVVTVARLVSA
                     DPALSARLLHLVNSPLFGQRGRVEDIVRAVALLGLQQVHDLVLAMSLQGIFAGVRPAR
                     LDMRRFWRNSLLTGLAARAAGIAAGLTAGERLFLIGVLADTGHLVMYQTVPELAAAAR
                     LAAQAGDEALDAAERRLVGCDFAELGAALLDRWQLPDRFGTAVGAQMLPRLGGAYAAE
                     AATINLARRIAEADELDLPSSAAAARVAPETWDLLGLEAERLAGLREGAEIDFAAYLS
                     LFFPRR"
     gene            complement(25675..26694)
                     /locus_tag="PKN25_05435"
     CDS             complement(25675..26694)
                     /locus_tag="PKN25_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNV90377.1"
                     /translation="MHTHHGHQHDHHAHNEHDHQHGDPAHGHAYPHQAGHAHPPHHAH
                     DHSASRFLPLALVLTLGFAAVEAIAGWWSGSLALLGDAGHMLTDAASLGLAALAARIA
                     LRPASARHSYGLRRVEALAALANGLFMLVIVGGLVWHAVERLLEPRAVAGEAVILVAL
                     GGLVLNLAVAWLLTRGEGDLNTRGALLHVIGDLLGSVAALASGIVILYTGWMPIDPLL
                     TMLICGLILYSTLALLRQVVHTLLEGVPDGLSLPEVGHAMAGVEGVRSVHDLHIWSLD
                     SNRSALSAHVVLADAARWPTVLAAERALLRSRFGIEHATLQPELPPAQPLVFTPRTPR
                     ARDAG"
     gene            complement(26803..>27045)
                     /locus_tag="PKN25_05440"
     CDS             complement(26803..>27045)
                     /locus_tag="PKN25_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV90378.1"
                     /translation="ADEVRKLAERTSKATTEISAMISTVQDATHLAISRVHGMHETAT
                     ASIGLAREAGEVIDGIHNGATEVVEVVERFSGLLHR"
BASE COUNT         3975 a         8741 c         9883 g         4246 t
ORIGIN      
        1 gcgcggccgc cgccggtctc acttcgcctc gaccttgccc cacaggcgca ggcgcgagac
       61 gccgccgtcg gggaagatgt tgaagcggac gtgggtgatc ggccccagtt tcgcgacctg
      121 ctccgtaaag gtatggatcg cgtccatctc cagcttctgc tcgggcagca gggtctgcca
      181 gaacatcgac tgggtggtga tcgagcggtc ggtgccgccg ctgacgtagg cggcctgcac
      241 cgagcagcgg tcggggtagt tgcccttgaa gaaggcggtg tcgacctcga tgcgctcgac
      301 cgtgccgggg gcgccgagct cgagcacgca ccagtcgttg ccgggctcgc ggcggcggcg
      361 ggtctcccag ccgtcgccca tgttcatgcc gcgcccgggc agcagcaggg cggcggcgga
      421 tgagccgaag ctggcgtcgt tccacgacag cgcgcggccg ccgttctcca tcgctaccag
      481 gtcgaccagc tcgccgccgg cggccttggc cgcgagtgtg cccaccgggc ggccgtagac
      541 gcgcaggcgc gcgatgccgc cgtcggggta catgttcacg cgcaggtggg tccacgccgc
      601 ggtgctggcg cactcgagaa ggtggtggct gttggggccc atcgggctgg cggggaggat
      661 ctcggtccac tcggccgcct gcagggcggc gacgtcgtcg gtctcggaca cgcaggcctc
      721 gatcgacacc gcgggcgggt agttgccggt gaagtgggag gtgtcgacgt cgaagccgcg
      781 gatcacgccc ttgacgccga gtttcacgag cgcccagtcg tggccggcga cgcgcttcct
      841 gcgcgactcc cagccgtcca tccacttgcc gttggtgtcg aacttgccgg ggacgaactg
      901 cgcgggttcg gggttgagca tgcggccgac ttcggcgaag aagtcgtcgg aggcggcgat
      961 cgccttggcg ccgaggcgcg ggttggcgag gtcgaccgag cgcagcgccc agtcgggcag
     1021 ctcggcagcg ggggcgaaga cgggggcgcc gggggtgtgg ctggccatgg gggttctcct
     1081 ttgggttcgg gtcgtgaggc aggcgggaaa gagccgggag tcgctcggga aatcgtggtc
     1141 tgtccccgat ttcccgattt ccccccggct tacttcgctt ggtaggggtg gcgctcgatc
     1201 cagtggcggg cgacgtcgac gcgacggcac acccacacgc ggtcgtgctt ctcgatgtgg
     1261 tcgaggaagc gctgcagcgc gcggaagcgc ccggggcggc cgagcaggcg gcagtgcatg
     1321 ccgatcgaca tcatcgcggg acgctcctcg ccctcggcgt acatcacgtc gaaggcgtca
     1381 cgcaggtatt cgaagaactc gtcgccgtgc gagaagccct gcggcagcgc gaagcgcatg
     1441 tcgttggtgt cgagcgtgta gggcacgacc aggtgcggca cggtctcgcc gttggtcttc
     1501 tgcacctcgg tccacaacgg caggtcgtcg ccgtagtagt ccgagtcgta caggaagccg
     1561 ccgtggtcgg cgaccaggcg gcgggtgttc ggggagtcgc ggccggtgta ccagcccagt
     1621 gcgcgcgtgc cggtgaggcg ctcgatgatc tccatgccga tcttcatgtg ctcgcgctcg
     1681 atctcctcgg gcacgttctg gtagtggatc cagcgccagc cgtggcaggc gatctcgtgg
     1741 cccagttcct tgaaggcggc ggtgagttcg gggtggcgct cgagcgccat cgacacgccg
     1801 aagatggtca gcggcaggcc gcggcgctcg aactcgcgca ggaagcgcca cacgcccacg
     1861 cgcgagccgt actcgtagat gccctccatc gacaggtggc ggtcggggta gctcgcggca
     1921 ttgaagagct cggagaggaa ctgctcgctg ccggcgtcgc cgtgcagcac gcagtgctcg
     1981 ccgccttcct cgtagttgag cacgaactgc accgcgatgc gggcgcggcc tggccagtcg
     2041 gcgtggggcg ggttgcggcc gtagccgacg aggtcgcggg ggtaggtcgg gttcgaggtc
     2101 atggtgttct gtccagacag gcgatcaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnc gcgcttcccg cgccgagatg ccgaccaccc gcgccctcgc ggcggccgtt
     2281 cagctgttcg tcccgaacat cgcatccagc cggaagcggg cgatcttgcc gatctcggcc
     2341 aggcaggtgc ggaattcggc gtcgcgcgcg ttggccaggc gggcctcgac gcggtccagg
     2401 atctggtggc gggtgaggcc cttgaccgcg accacgaagg gaaagccgaa gcgcgcgcgg
     2461 tagtcctggt tgagccggcg caggcgctgg agctcttcct tgctgcactg gtcgagcccg
     2521 gcgccgcgct gctcgccggt ggaggccgtg gtcagcgtgc cttcctcggc ttccttgccg
     2581 gcgagctcgg ggtgggcgca gatgaggccg agctgctcgg cgggggcggc ggtgtcgacc
     2641 accaggcaca tcgcggcgtg cagtgcgtcg atgctggcga agggaccggc gctccaggca
     2701 cgctcggcga tccacggcga gtgctcgaag atgccttcga ggcgggcgac gaaggcgtcc
     2761 tgcgccaggc gggagagttc atccagggcg gcgtccagcg cggcggccgg gcggtcgggg
     2821 gcggtggttg gggcggtggc gggggtgctc atggtgtctc ttcctgtcct gttcctgtcg
     2881 ttcagtccgc ggtgcgcgcg ccctgggcga accagatggc gatcttctcg cgctcctctt
     2941 cggtgatggc ggtgaggttg ccgagcggca tgtagccgct ggccacggtc tgcgcgctca
     3001 tcacggcgtg cgcggcgatc tgctcgggcg tctgcagcat cacgcccttg ggcgcctggg
     3061 cgaagccctc gaaggtcggc ttgtcggcgt ggcaggcgat gcagcgctgg gccatgatcg
     3121 ccttgacggt ggcgaagctc accttctcgc cgccggcatc gacctgcttc ggtgccatca
     3181 gcaccatcag cagcgcgatc agcgcgacgc cggccgccgg cagccaccac ttgacctggc
     3241 cgcggtggcg cagtacgaag aactggcgga tcagcacccc ggccagcatc agcacgccca
     3301 gcaccagcca gcccttggcg ttggcgtagg tcatcgggta gtggttgctg atcatgatga
     3361 acagcaccgg cagcgtgaaa taggtgttgt gcaccgagcg ctgcttgccg acgatgcccg
     3421 gcatcggatc gacctgctcg ccgcggcgga tctgctcgac catcttcttc tgcccgggga
     3481 tgatgtggaa gaagacgttg gccgccatca tggtgccgag catggcgccg acgtggatgt
     3541 aggcgccgcg ggccgagaac acctggtgca gcacgtagtt cgccaccatc acgaagacga
     3601 acacgatcgc ggcgagcacg ccgtccttgc cgatcatgtt gcggcacagc aggtcgtaga
     3661 acacccagcc ggcgaccagg aaggcgatcg agatgccgac cgcggcggca ggcgagagcg
     3721 ccatcacgct gctgtcgatc aggtagctcg aggcgccgat ccagtagatg acggccatca
     3781 tgcccatgcc cgacagccag gtggtgtagg cctcccactt cgaccagtgc aggtcctggg
     3841 tgagcggctc gcccttgggg ccggtgaggt atttctggct gtggtagaag ccgccgccat
     3901 gcaccgccca cagctcgccg aacacgccgc gcttggcgtc gttcgggttc ttcggcggct
     3961 tcagcgagtt gtccagcatg acgaagtaga aggacgcgcc gatccaggcg atgccggcga
     4021 tcaggtgcag ccagcggacc agcaggttgg cccagtcgag caggtaggct tccatggtct
     4081 gttctcttgt tctcgattcg gttcggtgtg gaggtggaag cgccgggttc agctgccgcg
     4141 gtaggtggaa tagctccacg gcgacaccag caggggcacg tggtagtgct cggcgacatc
     4201 ggcgatgccg aagcgcagcg cgacctcgtc cacgaacggc ggctcgggca ggttcaggcc
     4261 cagcgcgcgg aagtagtcgc cggcggcgaa gacgatctcg tacttgccgg cctcgagtgc
     4321 ggcgccttcg agcagcggct tgtcgcagcg gccgtcgtgg ttggtgacgc tgcgcgcgac
     4381 ttcgcggcgc tcgccgtcga gtcggtacac ggtgacggcg atgccggtgc cgggcttgcc
     4441 gtgggcggtg tcgaggacgt gggtggtcag gcggcccatg cgcttgtctc ctggtctggt
     4501 gcggggctgg gggaatcgtg tgagacgcag tgtaagccgg gtccgtgtta acggaacgca
     4561 tatgtcctta tatgtgatat atgctctcgc gtatctgtct gctggcggcc agtcgctccc
     4621 gccccggatc cacacaagaa cgacgaggag acccaccatg agcctgcggc gcagtgacga
     4681 cccgctcgac acctacctgt tgcgcgtgtt cgtgctcctg atcagcgagc gcagcgtgtc
     4741 gcgcaccgcg ctgcgcctga accagtcgca gcccgcggtg agcgcggcct tgaagcgtct
     4801 gcgcgagatc ctcggcgatc cgctgctggt gcgggagaag ggcggcatgg tgccgaccga
     4861 gcgcgcgctc gcgctgatgt cgcacgccaa gggcgcgctc gccgagatcg accgcatgac
     4921 cgatgcgccg gagtccttcg agccgcacag cacccgccac gagttccgcg tcggctcgcc
     4981 cgactatctc gcgccggtgt tcgtcgccag cgtggccgag cgcttccgcc gcgaggcgcc
     5041 gcaggcgcgc ctgagcctgc acgcgctggg gccgagcttc gacttcgagc gctcgctcgc
     5101 ggaaggggac ctggacgtgg tgatcggcaa ctggcccgag ccgccgcacc gcatgcacct
     5161 gtcgatgctg ctcgaggacg agatcgtgtg cctggtggcg gccagccacc cgtgcgcgaa
     5221 gaaggggatg agcgtcgacg actacctgcg cgccgcgcac gtggtgccga tgccgtattc
     5281 gatcagccag cgcggggtca tcgacagcgt gctggcgtcg atgcgcatca gccgcgacga
     5341 gcgcgtggcg gtgcagtcct tcaccgccgc gccctacctg ctgcagggca ccgatctggt
     5401 gttcaccacc acccggcact tcgcgcagtt ctacgccaac ctgctgccgc tggcgatcat
     5461 cccctcgccg atcgcctttc caccgatgcg cttctaccag ctctggcacg agcgcaacca
     5521 ccattccacc ggccaccgct ggctgcgccg actgttgtcg gattgcgggc gcaggatcgc
     5581 gccaccgcat gcgtgagcgg gatgggggct tgcggtccgc gtggtgcctg cggaggagcg
     5641 cggtgtttct ggaaacggtg gatcgcgact nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn accgcagcgc tcagcggtcg atcggcacca gcatcaccgg gatgtcggcc
     5821 ttcatggtga ccttgtgtgc caccgagccg agcagcagct cgccgatggt gctgtggccg
     5881 cgcgagccca tgacgatcac gtcggcgagc acatgcttgg cgacgtcgag gatgcggcgc
     5941 gaggggtcgc cctcgagcgc gcgcacgctg gcgagcacgc tcttcacgtc gagctcgggg
     6001 ttggcctcgg cgaagacgtc gatgcgatgg cgcagcaggg cctcggcctt cttgtggccg
     6061 gcgagcttga tctcgtccat cttctcctcg ctgaggaact cctcgtaggg caggctgctc
     6121 gatggaaggg tcacggtgac ggcgtgcagc ttggcgttga acttcatcgc caggcccacg
     6181 gcgtggcgga ataccgcggg cgagcgcggg gtcaggtcgg aggcgtaaag gatcgagcgg
     6241 atttcgtagc tcatcgaggg aactcccggt tgcggtggtg tggtcggtgc cggagcgcgg
     6301 tgtcccggcg ggcatcctgc attctcgtgg ccggtggatc cggcgatgcc tgcgcaggcg
     6361 tcctggacaa agtgtagagg cgtgcgccgc gccggcgcag ccgcgccgcc cgatagcgca
     6421 tatacggaat gcgtgatgac gctcacacgt gcggcctgct tgcggccggg ccggcacggg
     6481 cgggatgatg atgcaagaac gggccgcggc ccgcagccat ggagaatcgc atgatcgatc
     6541 tgtacaccgc cgcaaccccc aacggccaca aggtctcgat cgcgctcgag gagctcggcc
     6601 tggcgtactc gctgcgcgtg ctcgacctgt ccgccaacga gcagaaggag ccctggttcc
     6661 tcgccatcaa ccccaacggt cgcatcccgg cgatcgtcga ccacgacgag ggcgacttcg
     6721 cggtcttcga gtcgggcgcg atcctgatct acctcgccga gaagaccggc cggctgatgc
     6781 cgcaagacgc cagggggcgc tcgcgcgtgc tgcagtggct gatgttccag atgggcggca
     6841 tcggcccgat gatgggccag gccaacgtct tcttccgcta cttcccggag aagatccagc
     6901 ccgcgatcga ccgctaccag ggtgaggtca agcgcctgtt ccgcgtgctc gacggccatc
     6961 tggcgaagaa cgagtacctc gccggcgact attccatcgc cgacatcgcc aactgggcct
     7021 gggtgcgcac gcacaactgg tcgggggtgg cgatcgacga cctgcccgac ctcgcgcgct
     7081 ggcgcgacca gatccgcgcc cgccccgccg tgcagcgcgg catcgagatg ccgccgtcca
     7141 gcttcgaccg cgacggtgac ggcgaggaac aggccaaggc gttctcggcg aaggtgcgca
     7201 acatggtcga gaccggccag tcgcgcagcg gtggagtctg agggcccccg cggccctgcc
     7261 ggttcaggac attccaggag accccatgaa gctctatgta tcaccccggg cgcccaatcc
     7321 gcgccgcgtg cagatgttca tcgccgagaa gggcatccgc ggcatcgagg aggtcgcggt
     7381 cgacctcgtc aagggcgagc accgcagctc ggaccaccgc gcacgcagcc cgctcgccaa
     7441 ggtgccggtg ctggaactcg acgacggccg ctacctcggc gagacgcggg cgatctgcag
     7501 ctacctcgaa ggcctctacc ccgagcccaa cctgatgggc gaaagcttcg aggagcgtgc
     7561 cttcgtcgag atgaccgacc gccgcgtcga gctctacctc ttcggcggca tcatgaactg
     7621 cgtccgccac acccatcccg ggctggcgcc gctggagcag ccgcagttcg aggatttcgg
     7681 ccgctcgcag ggcgagaagg tgcgcgaggt ggcgcgctgg ctcgacgagg agctcggccg
     7741 ccagcccttc gtcgccggcg agcgcttcac catcgccgac atcacggcct tttgcgcgat
     7801 cgagttcgcg cgcgggctga tgaggttcag gcccggcgcc gagggcatgg ccaacctgca
     7861 ggactggcgc gaccgcatcg ccgagcgccc gagcgcgcgg gtctaggtgc gcggctgcgc
     7921 gggaaggatc aggaacccgc gcgttcgcgg cggctgcggt ccagccagta gagcagcttc
     7981 gagtacagca cggcgggcat gatcggcttg gtcaggaagt cgtccatgcc ggcttcgagg
     8041 cagcggaccc ggtcctcggc gaaggcattg gccgtcaggg cgatgatcgg cacctcgcgc
     8101 ccccagtcct gggtccggat cctgcgcgtc gcggcgatcc cgtcgacaac cggcatctgc
     8161 atgtccatga ggatcagggc cggcttgcag cgttcggcga gcgccgcggc ctcttctccg
     8221 tcggctgcgc attccggcgc cagcccgccg cgcgacagca gcacccgggc gatttcccga
     8281 ttgaccttgt cgtcgtcggc cagcagcacg catgcgccgc catgccgctc ggcgagcgcg
     8341 ccgcggatct ccaggggcga acccgtgggc gccacgacct cgtcggcctt caccttgcgc
     8401 aggcgcgtgg tgaaccagaa tcgactgccg cgaccaggct cgctctcggc gcccgcctgc
     8461 ccgcccatca gctcggcgag acgcgcggtg atcgccagcc cgagcccggt gccgccgtgc
     8521 tcgcccgtcg aggtcgcctc ggtctgggtg aagggcttga acagacgccg cagttcggcg
     8581 gcagcgatgc ccggcccggt gtcctcgacc acgaagcgga cgtccaccgc gtccgtgtcg
     8641 accgcgctgg cgctgcagcg cacggtgatg ccaccccgct cggtgaattt cagcgcgttg
     8701 atgaggtagt tgaccagcgc ctggcgcaga cgcgtcgcgt cgccgaggaa ggtgcccggc
     8761 ggcaggtcga actctgcacg caggctcagc cccttggcct cggcctggtg ggcgagcatc
     8821 gccatcgcct cctcgaccac gccgcgcagg tcgagtgcgg cctcgtcgag cgtgaacttc
     8881 cccgcttcga tcttcgacag gtcgaggatg ccgttgatga tcgagagcag gtgcctgcct
     8941 gcgttctcga tcacgtcgag ccgctcccgc tgggacgccg gcaactcctc ctggtgcatc
     9001 aggtgcgaca tgcccaggat tgcagcgagc ggtgtgcgga tctcgtggct catgctggcg
     9061 aggaaagacg atttcgcctc gctgacactt tccgcgttct ccttggcgcg cttgagcgcc
     9121 tcgtgcgcct tttgcagttc ggcttccgcc tggcggcgac ggttgatctc ccgcgtcatc
     9181 gccatcgtgc ggttggcgag cttgtcgtac atcaccagca cggtctcgac caggacgcgc
     9241 atcgaacccg acatggtttc gtcggcttgc gtcctcgcct gctcgagcgt caatccgcgc
     9301 tgcacgccgt gtacgaccag ggccatgcgg cggtccgcct cgaggatgtg cagtgccagc
     9361 cagcgggtca gaaagttgac gatgttctcg atcaccgtct cgaagggttt ggtggattcc
     9421 tccgccttca gacgcaggac ctcgtcgatg aaacggacgt gagattcatg gtggcccgcg
     9481 acgccggcgt actcgcccag atgcgcctgc cagagcgcct cctcggtggt gaaatggacc
     9541 tcgacgtaat cgcgcagctg gtcgaacacc gaattgagcg tgggggcgtc ggattggaag
     9601 gcgagatgcg tgacgagacg gttcagcagt gccacgagct gctggtgctg tgcatcgatg
     9661 accgcgatgc cggtggcgaa gttgctgtcc cacgggaaga tctccatctc ggtgatcatt
     9721 gctgcggtgc tccgggcaga ggatggcatc ttgaaggtgg cgaatattgt aaatattcca
     9781 gatatggcga cgatggttga tatttcacgc gtcgacgcga attctgccgg ctgccggcct
     9841 tgccctcaaa cgccctcctc acggggcgag gtggcgagcg agcttctgca gcaggatctc
     9901 gcggtcgaac ggcttcacca ggaagtccac cgcaccggcc ttgaggctgg cttcgagaac
     9961 ctggcgttcg ctgtgaccgg tgatcatcac caccgggatg gccgcaaaac gcgggatggc
    10021 cttcaggcgt cgggtgagtt ccacaccgtt gaagtcgggc agcgcgacat ccatcaggat
    10081 gagatcgggg ggctggcggc cgagcaccgc aagggcttcg gcacccgagt gcgcgatgat
    10141 cgaacggtag ccggaattgc cgagcaggcg ttcgagcagc ttgcactgga aggcgtcgtc
    10201 gtcgaccacc acgatgaggg gcggtgtcct gccagcgagt tcgccgagtt ggcgagccgc
    10261 atcgagttgc ggcttgacgt ccgccttgag cccgccgatc cattcggtga gcggcaccac
    10321 cttctttttc gcctcgtcca cacgcagctt cgcttcctgg atgacgtctt cttgcggtgt
    10381 cacggcgaac tgggtcggcc gatccaggcc cgagggcacg tcgccgacgt cgagctcgaa
    10441 gctgtccgtg aatgcgggca ggacgtcgag gcctgcgtcc gggatggtgg cgctgtcgct
    10501 ggagggcgct gcgggcgccg cagggcgggg ctgcacgctg gaggcttcga tttccgctgc
    10561 ctgaagtgcg cgctcgagcg cgtccgcatg ctggcgaccg acctcgatgc gatcgtccag
    10621 cacggtctcc agctggctga tcgagcgtgc atgcgcggcg acctcgcgtg cgttcggcgt
    10681 gtcccgctgg gcctgcaacg agcggccggc gatgaggacc gacatggcca ggcgtggcgt
    10741 gtcgaacacc atgggccagt acaggacgta gtcgtcgaag tactccttgc ggcacagctc
    10801 gaatgcgcga tggacgttgt ccttgtcgca cagcacgatg ctgcggtgag gcagggagtg
    10861 caccacctcg ctgtgacgat agaggccgag cgagtagcgc tcggcgcgct cgaggctggt
    10921 gaaggccagc accacgacgt cgggctggac gcgctcgaaa tcggcgatga agcggctctc
    10981 gatcgtcgac gtctcgatgt gctcgaaatg gtccgcgagc aggcgcgcga tctgggccgc
    11041 atcggccgcg acgtcggtaa cgaccaagac cttgagggtc tcgaggttca tcggttccgg
    11101 ctctgtcggt gggatccgta ttctagcggc tacccaggcc ggggggtatc gccacgcgca
    11161 ggcggaactc ctgcggcctg tgtgccttgc atcgcggtga gcgtggctgc cagggccgcc
    11221 tcgagcagcc cgccctggcg ctgcacttgg tcgccatcgt cgtcgcgacc ggcctgttcg
    11281 agcgcggcgc agatttcgcc gagctgcatg gcgccgaccg agcgcgagga ggacttcagg
    11341 cggtgggcga gctcgccggc ccggcgccag tcgccgtcct cgcacgcctt gcggatgctg
    11401 cgcacggtgt cgcatgcgga gagccggtat tcgccgagaa actccgcgat cagcgccgca
    11461 tcgttgccga tcagtcccgg caaggcctgc ggatcgaaca ccgccggggg cgccgccggg
    11521 gccgccggcg cggtgccggc gggagtccgc tgctcggtgt ccggttgcag ccagcgcgcg
    11581 agcacctcct gcagcgccgc aacctgtacc ggcttgagga tgaagtcgtc catgccggcg
    11641 tcgatgcagc gctccttctc tccgcgcagg gcgtttgcgg tgaccgccac gatcggcgta
    11701 cgccggcccg gcgcttcctc gctgcggatc ctcgccgtga gttcgtagcc gtccatcccg
    11761 ggcatgtgga gatcggtcag cagcaggctg taatgaccct gccgccagcg ctcgagtgcc
    11821 tcgaagccat cctcggcggt gtcgcattgc agcccgagga gtgcgagctg tcgcgcgatg
    11881 acgcgacgat tgatgtcgtt gtcctcggcg acgaggatgc ggcgaccggc ctcgccggct
    11941 ccgggtgtcg caagcggcgg cgtgggcgag gggcgagcga gcgccgtgct gcgggctggc
    12001 tcgctgcgcg tggccgagcc gttggtcgcg gcaagcggga gcgtgaccac gaagcggctg
    12061 ccacgcccgg gaaggctgtt cacctccatc cggcccttca tcatgcgcac gaggcgcgtg
    12121 cagatcgaca gtccgagtcc gctgccgccg aagcggcggg tggtgctgga ttcggcttgc
    12181 gagaaggggt cgaagatctt ctcgatcgcc tcgggggcga tgccgatgcc gttgtcggtg
    12241 accatgaagc ggatccggcc tgcgccggcg gtctcgacgc gaatctcgac gcggccgggg
    12301 cggtcgaggc cggaggagaa cttcaccgcg ttgccgacga ggttgcacag gatctgctgc
    12361 aggcgcagcg cgtcggtgcg cacgaccgcg ggcacgtcgg ggtcgatgcg ggtgctcagc
    12421 cggaccgact tgcggttcgc gaccgtctgc aggatgccga cgacacgcgc gatcaggtcg
    12481 gggatcgatg cagggccgac gtcgagctcg agattgcccg actcgatcct ggaaaagtcg
    12541 aggatgtcgt cgatcagccg cagcagggtc tcggcggatt cgcgcatgat gccgaccatc
    12601 tcgacctgtt ccgtggagag ctgcgaatgg ctcagcacgt cgagcaggcc gatcaccccg
    12661 ttcatcggcg tgcggatctc gtggctcatc gtggtgagga actcgctctt tgcgcggctg
    12721 gcgatgttgg ccgcatcgag tgcgtgctgc agttcggccg tccggctctc gacgaggccc
    12781 tcgaggtgtt cgtggtagcg cagcagttcc tcttccatgc gccgttgcgc ggtgatgtcc
    12841 tgcttgaccg cgacgtagtg ggtgatgtgc ccgctttcgt cgtggatcgg cgtgatcgtc
    12901 acgcgctcga ggtactcgct gccgtccttg cggcggttga agaactcgcc ctgccaggag
    12961 cggccgtggg tgagcgtgtt ccacaggtcc gcataggtgg ccggggtggt gcgtccggac
    13021 tgcagcaggc gcgggttctg gccgatggcc tcggcacggg aatagccggt cttgtcgatg
    13081 aaggcctggt tgacgtactc gatctcggcc cgggtgttgg tgatgacgat gctctccggg
    13141 ctctcctcga cggcgcgcga cagcttgcgc agctcatcct cggccgcctt gcgcgcgctg
    13201 atgtcgagtc cgaatgcgaa gcgcgcgacg gcgcggccgc ggtcgtcgag cagggcggtc
    13261 actccgatca cgagcggcag ccggctgccg tccttgcgca ggtgaacggt ctcgaaactg
    13321 gcgttgccga tgcgatcggc ctcggcgagg tgagcatgga gttgctcgcg ctcgtcctcg
    13381 gggtaaagtt cgtcgatccg catgccgcgc agctcctcga tcgagtagcc gtgctggctc
    13441 gcataggtgg cattgacgtc gatgatcgtg ttgtcggaca cgtcgtgcat cgcgaggccg
    13501 aggttcgaca ggcggaagaa gcgctcccag cgtcgcgaca acgcttcggc ctgcttgcgc
    13561 tcggtgatgt cgtgcacgat cgagaagacc atggagcggt tgccgtcctc ggtcggtccg
    13621 ctgtggacct cgacgtcgcg gacggagccg tcggcgcggc gatggcggaa ctcgaagtgc
    13681 ttgcgctctt ccgcatgggc cgccttcagt tcgacgagca gcgcctgcgg ggacagggtg
    13741 ttgatgtccc cgatctgcat cgtctgcagc gtttccctgg accagccgta gaactcctcc
    13801 gcggcggggt tggcgtcgag gatgcgaccg ctctccgggt cgaggatcag catcaccgcg
    13861 tggctgttgt tgaagatgtg cgcgtagcgg gcttcgctga ggtcgcgcgc gcgctcggcc
    13921 tcgagctgtc gggtgaggtc cttcgcgaaa gagagcagcg agacgagccg gccttgcggg
    13981 tcgcggttcg cccggttgtg ccattcgcaa tgcagcaggc ggccgtcgcg cgtgaagttg
    14041 cggccgatca ccggcggcgt gggaccgtcc gcggtgatgg cctggccgat cgcgcgcttg
    14101 accgccgccg cgtcgtccgg atgggtgaag ctccattcgt tggggcgcct gccgagcacc
    14161 tccgcagcgg accagccgaa aacccgctcg gcctcgggcg accagcgcag gacgcgcatg
    14221 tcgcggcccc actcgatgac cgcgaacggg caggcctcga tgaaaagctc gatcgctgcc
    14281 gcggaggggg gcaaggctgg cgtcggggca gggaggctca cggtggcgcg ctgtgccggg
    14341 tgcggatcgt tcatcggtcg cgatctgccc cgccccgggg ctgcgcgccg ctcccgtgca
    14401 aggcgtccgt cgcggacatc cgtgcgcgga agctctccac cgcctcgggg cgggcgaaca
    14461 ggaagccttg gaaggcgtcg cagccgttgt cgagcaggca ggcgtgctgt gcggaggttt
    14521 ccacgccctc ggcgacgacg cggatgcgca gggtgtcggc gagtgccatg atggtgcgca
    14581 cgatcgccag gctgctggcg ttggcgggca ggtcgcgcac gaaggtgcgg tcgatcttga
    14641 gctcgtcgag cggcaggcgc tgcaggtagc cgagcgagga gtagccggtg ccgaagtcgt
    14701 cgatcgagaa gcggatgccc atctcgcgca ggcgggtcat ggcctcgatg gcgcgctcga
    14761 tgtcggtgag cagcagcgat tcggtgagtt cgagcttgag ccgctcggcg ggggcgccgg
    14821 tgcgcgccag cagcgcgagc acctcctcga cgaaaccggg ctcgtagagc tgggcggcgc
    14881 tgacgttgac cgccatcacc aggccgtcgg cgccggggag cgcgacccag cgcgcgagtt
    14941 cctcgcaggc gagcgccatc gcctcgcgtc cgagcgcagg catcaggccc gcctgctcgg
    15001 cgatcgggat gaacagggcg ggcggggtcg gtccgtgccc ctcgcggttc cagcgcagca
    15061 gggcctcggc gccgatcagg cgtccgccgg catcgagctg gggctgcagg tggagcagga
    15121 acgcgccggt gccgatcgcg ccgcgcagct cggtctccag cgcgagccgg tcggccagct
    15181 cggtacgcat gtgcggatcg aagacgcggg cggtgttctt gcccgagcgc ttggcctcgt
    15241 acatcgacag gtcggcgcgc tgcatcagtt cgtcggccgt gtgctcgtgg ccgttgaaga
    15301 tcaccaggcc caggctcgcg gtgcatttgc ggtcgagtcc gtcgagacgg tagggatggc
    15361 cgagctgttc gagcagtttg tccgcgacct gacgcgcggc gagcaaggcc tcgtcggcag
    15421 attcggcgag cagctcgagc aggaccacga actcgtcgcc gccgaagcgt gccaccgtgt
    15481 cggattcgcg caccacgccg cgcaggcgtg cgccggcctc gcgcagcagc acgtcgccgt
    15541 tgtggtggcc gagcaggtcg ttgatgtcct tgaagtcgtc caggtcgatg aacatcagcg
    15601 cgccgtgcag gccggtgcgc gcattggtgg cgatcgcgtg gccgaggcgc tcgacgagca
    15661 ggcggcggtt gggcagcgtg gtgagcgggt cgtagtaggc gagcttgcgg atgcgctcct
    15721 cggcggccac gttgggcgtg atgtcctgca ccaggatggc cgccgcgcgg cgcccgccga
    15781 tcggggcgtc gatggcctgg atccaggcct ggaattcgcg gtggccggcg ggcgtgtcga
    15841 gcgcatagcg ggcgcaggtc cgctccgggc tggccagggc ttgctccagc gccgcgcgca
    15901 gcacggcggc cggtcccgcg ggcagcacgt cctcggagcg tcgaccgatc acctgctcgg
    15961 gcggtgcggc gagcaggttt tcctggcggg tgatgacctc gaggtagcgg ccgtcctcgt
    16021 cgaccacgaa gaccaggtcg gggatggccg cggtgatcgc gcgcaggcgc gcctcgctcg
    16081 ccgccagcgc gcattcggtc gcgcggcgct gctcgaggtc gcgcagcagg gcgccgagca
    16141 gcagcgtggc ggcccccatc agcagcaggt agggcaggcc gaggttggcg aagagaccgg
    16201 cgagggactg gttggggacc gttgcgatca gcagcaggct ggtggcgtgg gtgaatacgc
    16261 cgaagaggaa gagctgcagc ggcgccggcc gcagccagcc gcgcgcggcg ccgaggcgga
    16321 aggcgaggcc gagcagcacc gccagcgtga tcgtcgtcac gccgatgaag acgccgctgc
    16381 cgcccagcca gatgcgatag gctgcggcgg ccaggccggc cacggcgccg gcgagcggac
    16441 cgccgaagag tgcgcccacg ctcaacacca ccgagcgcgg atccacgatg acgccgggcg
    16501 ccagcgtgat cggcatcatc atgccggcga tgcaggccag cccgtagatc agcccggacc
    16561 cgaccgcgaa tgccgggccg ccgtgcgcgc agcggcgtgc caaaccgccc tgcagcaggc
    16621 agagggcgag cagcagggcg gtggcctgga gcaggtcgag gatcatcggg cgggtacggg
    16681 caggcggacc tgcgccggag cgtgttcgga tgccgcagat gctagcccgc gctccgcccg
    16741 gctgctgccg actgcgtcac gcgcgacccg agatgggttg tcccgcgctg gagggctgcc
    16801 gagccgccgc ggtcgcggcg aaaggcgtca ggcctcgggc cggtagggct tgccgagcga
    16861 gaccggcgag ttgagccgga tcgtgttgcc gttgcgcgag atcgccacca gcaccacgat
    16921 ggtcgcccag tagggcaggg acgacagcag ctgcgagggg aagtcgatct gcagccccga
    16981 gccctggatg aagagctggg tgaccatcac gccaccgaac agataggccc cgaccatcac
    17041 ccgcagcggc cgccaggtcg cgaacaccac cagcgcgagc gcgatccagc cgcgcccggc
    17101 gaccatgccc tcggcccaca tcggcgtgta gaacaccgac aggaaggcgc cgccgatgcc
    17161 ggccatggcg ccgccgaaga gcaccgcgag gtagcggatg cggatcaccg ggtagccgat
    17221 cgcgtgcgcg gatgcaggcg actcgcccac cgcgcgcagc accaggccgg cgcggctgcg
    17281 gtgcaggaac cacagcaggc cccagaacag cgcccaggag aaatacacca gcgcctgctg
    17341 gttgtagagc gcttcgccga tcagcggcag gtcggcgatg aaggggatgc ggatcggcgg
    17401 caccgcctgc agcgccaccg cctcgtaggg cttgccgacg aaggccgcga ggccgacgcc
    17461 gaagatcgcc agggcgaggc cggaggcgac ctgattggcc atcagggtga gcacgagcag
    17521 gccgaagagc gccgccatcg ccatgcccgc gcccatgccg gcggccacgc ccagccacgg
    17581 gttgccgctc tcgtgggtga cggcgaaggc agcgaccgcg cccatcgtca tcatgccctc
    17641 ggcgccgagg ttgagcaccc cggccttctc gttcaccagc aggccgaggg cgacgatgat
    17701 cagcggcgtg cccgccacca cggtggcgaa cagcatcgag gtgaagagac tcgcgtccat
    17761 ggcggcctcc tcaagtgcca cgccggcgca ggcgcaggcg gtagtggatg aagacgtcgg
    17821 cgccgagcag gaagaagagc agcatgccct ggaacaccat cgagatcgag ttgggcaggt
    17881 tcaggtattg ctgcgcctgc tcgccgccga tgtagagcag ggccatgagc aggctggcga
    17941 gcaggatgcc gaaggcgttg agccgcccga cgaaggcgac gatgatggcg gcgaagccgt
    18001 agccgctgcc gaccttgtcg gtgagctggc ccatcggccc ggccacctcg cccatgccgg
    18061 ccagcccggc ggcggcgccg ccgaggagca ggcccaccca gatcatgcgg gtggccgaga
    18121 agccggcgta gcgcgcggcg tcggcggcct cgccggcgac ccgcatgcga aagcccgcgt
    18181 ggctgcggtt catgaaggcg tagccggcca ccagggcggc gagcgcgatc gcgaagccga
    18241 aatgcaggcg ggtgccctcg atcaccatcg gcagggtcgc cgcctcggcg aacatccgcg
    18301 tctgcgggaa gttgaagccg tcggggtcct tccatggccc gaacaccagc caggacacga
    18361 agagctgggc cacatagacc agcatcagcg acaccaggat ctcgttggcg ttgaagcgcg
    18421 tgcgcagcag cgccgggatc gccgcccaag ccgccccgcc cgcggcgccg gcgaggacca
    18481 tggccggcag caccagggcg ctctcggcgt cgccgaagta cagcgccacg ccggtggcgg
    18541 ccaccgcgcc gagcatgaac tggccctcgg cgccgatgtt ccacacgttg gcgcggaagc
    18601 cgatcgccag gccgatcgcg cacagcatca gcggcgtggc cttgagcagc agttcggaga
    18661 gcgcgtagag gtccttgacc gggttgagga aaaagacgcg gaagccttcg gccgggtcct
    18721 tgccgagcgc ggcgaacacc gccaggccgc cgacgagcat cagtgccacc gcgagcagcg
    18781 gcgaggccca ggacatcagc cgcgagggtt cggcacgggc ttcgagctca agcatgcggg
    18841 gtctccgtcg cgaggggcgc gccggcagtg tgctcggcgc ccggccacag cccgctcatc
    18901 cacacgccga tgtcctcgat gctggtgtcg cgggcggcgc gcaccggcga cagccggccc
    18961 ttggcgatca ccgcgatgcg gtcgcagatc tcgaacagct cgtcgagctc ctccgagatc
    19021 accagcaccg cgcagccgcg gttgcgcagg tcgatgatcc gctggcggat gaaggcggcg
    19081 gcgccgacgt ccacgcccca ggtgggctgc gcgcatacga gcaggcgcgg ggcctgcagg
    19141 atctcgcggc cgacgatgaa cttctgcagg ttgccgcccg acagcgactt ggccgcggcg
    19201 gccgtgccgc cacagcggac gtcgaactcg tcgatgcagc gccgtgcgaa cgcgcgcgca
    19261 cgcccgaagc gcaggaagcc ggcggccacc atgccctggt tgtgcgcggc ggtgaggagc
    19321 gcgttgtcgg cgagcgacat cgtcggcacc gcgccgcggc cgaggcgctc ctcgggcacg
    19381 aaggccatgc cgagccgccg ccgccgcccc ggcgacagcc ggccggcctc gacaccgcag
    19441 atctgcacgg ggaatttctc cgccagcggc gcctcgcccg agatcacgcg cagcagctcc
    19501 tgctggccgt tgcccgagac tccggcaatg ccgacgatct cgccggcatg gacgtcgagg
    19561 tggaggtcgc gcaggtcggt gccgaagggg tcgtccgcgg cgtgcgagag cccggccagg
    19621 cgcaggcgga cttcgctgcc ggcctgccgc gcgtcgtgct cgcagcgcgg cagttccttg
    19681 ccgatcatca ggcgcgccat cgaggcgggg gtctcctgcg ccgggatgca ctcgccggtg
    19741 accttgccgc cgcgcagcac ggtggcggtg tggcagagcg tgcggatctc gtcgagcttg
    19801 tggctgatgt agaggatcga gcagccttcg tcggcgagcc ggcgcagggt ctcgaagagc
    19861 ttgcgcaccg cctgcggcgt gagcaccgag gtgggctcgt ccatgatcag caggcgcggg
    19921 tcctggagca ggcagcggat gatctcgacg cgctggcgct cgcccaccga cagggcgtgg
    19981 atgtggcggc gcggatcgac cggcaggccg tagcgctccg agacctcgac gatgcgccgc
    20041 gcgagcgcgt cgagcgcggg cttgccgggc agggccaggg cgacgttttc caccacggtc
    20101 agcgtctcga acagcgagaa gtgctggaac accatgccga tgcccaggct gcgcgcatgc
    20161 gccgggctgg cgaccgcgac ctcctcgccc tcccagaaca tgcgcccgct gcttggccgg
    20221 gtgacgccgt agatgatctt catcagcgtc gacttgccgg cgccgttctc gccgaggatg
    20281 gcgcggatct cgcccgcgcg cacgacgagg ccgatgtcgt ggttggcgac caccgtcggg
    20341 taggccttgc tgatgccctg caggaccagt cgggcagggc cggcggcggt gtccggctgc
    20401 agggggggat acgggatggg cacgtcgatt cgaagcggtg tgtcgtcggt cggaaagcgg
    20461 gcgcgtgcgg cgtcgtggac gcccgggggc tcagcggctg cgctcggccg cctgccgggc
    20521 ggcctcttcg gcggcttcga gcgacagggc ctgcgcgcgc cggagctcgt ccgcgtcgag
    20581 cttgaccgcg atgctgtcgc gctcctgcac gccgtccatg ttgagcaggg cggctgcgcg
    20641 attgaaccac acataggcct gcaccgcatc ccgttcgacg ccgatcccgc tgcgatgcag
    20701 gcggccgaac tccaccatgc cctcgggatc gcccgcatgc gccgcctggt tcagccagcg
    20761 cgcggcgagc tcgtagttct gcggcatgcc gatgccgctg cggtagagct tgccgagcag
    20821 caccatggca ccggtgtggc cgccttcggc agcctcgcgc aactggctca ccgcgctggc
    20881 ggacacttcg cgcccgcgcg cggtgtcgag cagcgcggca cgtgcggcgc ggaagcgggc
    20941 gtcgagcgcg tccgtcggcg ccgagggctc ggccgcggcg gatcgggtct cgcccggcgc
    21001 atcgccggac tcgtcgaggt ccagcatgcg cacgctcgcg atgccgatgc ccaggagcag
    21061 cagggccgac aggatcccca ccaccagcgg ccatctgctg cgcggccgga gcgcgtcgtc
    21121 gggagccagg aagcgctggt cgcaggccag gcagcgccag gggtgcagcc cgggatggcc
    21181 ggcgtgttcc tcgtgcgagc gccagcgcga acgccggcag tcggtggatc cgcaggccgg
    21241 gcaggccgga tgggcgcgag tgctcatgcg cgctccccgc tcagctcttc cagaaccaca
    21301 ggatcgcgat gacggcgatc gtcagcagca gggtgctgcc gcccaccgcg gcgacgatcg
    21361 ggttgtcgcg cagcaggcgg cgcggctccg gggcgtggaa gcggtgcacg caggacatgc
    21421 agcgccatgc gcgctcgccc gggtgggcga ggcgctcgtc cgcggatttc cacggggact
    21481 cgcggcagtc ggttccgtcg caacgcgggc aggtgggcat ggtgatcgct cgggcgtgcc
    21541 gtggtcgcag ggcgggctgc gcaccgtttc tgaagatttt ctcgattata tagagcgtct
    21601 gcgtccaaga aacgatcgac ggatcacacc gagcgcaaaa aagggcagcc cgcagggctg
    21661 cccttttttg ctgaatatga cgtttgcgcg atgcccgtgc gcgcgctcag ccgcaggtcc
    21721 cgaccgcgcc gcaggcggtg cagaagtcgc agccgtcctt gcggatcacg gtgtggttgc
    21781 cgcattcgtt gcacagcgcg ccctgcatca gcttgggctc gttgtcgtcg cgcggcgact
    21841 ccaggatgcc catctcgcgc gtcgggtagc cgcgctcgtc gagcacgccg agcatggcgt
    21901 agcggtggat gatgagctgc gccaggtagg ccacggtgct gggccagttc tgctggcggc
    21961 gggtgccggg cacgaaggcc atgaagtcgc cgagcggctc ggggtagtcg aggagcttgc
    22021 gcagcttcat gccgatccag gccgggtcca tgacgcgcat gtcgagcgac aggatgcggg
    22081 tcaggccgtc cagcgcgcgc ggataattgc ccgacagcca caccgagtac gggcgggtga
    22141 cgccgtccgg cagggtgatt tccttgaggc cgagcacgaa gtcttcgccg gtggccgggt
    22201 tgtagatgtc caccgtccac gacagcgtgc cgtcggtgcc ggtcttgggt tcttccaggc
    22261 tgaacagcgt gtcgaggacg ccgttgctct cgtcttcctt gtcgaacacg ccgagcttct
    22321 cgcagcggta attcaccacc tgggcgaagg ccgacaccac gccgggcacc agcttcttct
    22381 cgccgtgcgg cgggaagggc atctcgaagg agcgctcgcc cacggtgcgg gccagggtgt
    22441 cgagcttgag cttgagccag ccgcggtcgt tggcgcgcat gtccatcgac agggtcttgg
    22501 ccaccgcacc caggccgcgc ggctggtcgg cgccattgac ccacacctcg aacgggaagc
    22561 tgccgccgtt gttgcccacc tggccgacga agagcgcgaa gccgccggtc ggggtgttga
    22621 gcatgtaggt ccacgccagg ttgccgtcgg gcaggtccgg gcggccgggc cagcgcaggc
    22681 tggacagcac cggagcgggc aggttcttga tcgacaggcg cttgttggcg tcggcgatct
    22741 cgacgtcgtg cggctgcttc tgctcggcgg tcggggtgac cgacagcacc gaacccagca
    22801 ccgagttggg acggtaggtg gcgagcccct tgaggccggc tttccaggcg gtgaggtaaa
    22861 gatcctcgaa ctcggcgtag gggtagtcct cgggcacgtt caccgtcttg gagatggacg
    22921 tgtcgacgta gggcgcgacc gcggcgacca tgtccttgtg ggcctgcgcg gagatctcca
    22981 gcgcggtgac gaaggactgc ggcagcttgt cgacgttgcc gccgaggtgc ttgtacagac
    23041 gccaggcgta gtcctcgacc gcgtactcct tgaaggtgcc gtcggccatg cgcttgcgcc
    23101 gggtgtacgt ataggagaag ggcggctcga tgccgttgga ggcgttgtcg gcgaaggcca
    23161 ggctgatcgt gccggtgggg gcgatcgaca gcaggtgcga gttgcggatg ccgtgcttgc
    23221 ggatcttgtc cttcacatcg gccggcaggc gcgaggcgaa gttgccgccc gacaggtaga
    23281 ggtcggcgtt gaagagcggg aaggcgccgc gctcgcgggc gagttcggac gaggccaggt
    23341 aggcgcggtt gcgcatgaac tcggagatct tgcgcgcttc ctcgcgcgcc tcggcggtgt
    23401 cgtagcgctg gccgagcatg atcagcgcgt cgcccaggcc ggtgaatccg aggcccacgc
    23461 ggcgcttgga ctgcgcctcg gcgtgctgct gcgcgagcgg ccagtgggtg acctcgagca
    23521 cgttgtcgag catgcggatg gagacgtcga ccaccttgcc gaagccggca tagtcgaact
    23581 cggccttgtc gccgaagggg ttcttgacga agagcgtgag gttgatcgag cccaggcagc
    23641 agcagccgta gggcggcagc ggctgttcgg cgcaggggtt ggtggcctcg atggtctcgc
    23701 agtagtacag gttgttgtcc gcgttcatgc ggtcgaggaa caggatgccc ggctcggcgt
    23761 ggtcgtaggt cgagcgcatg acctggtccc acagctcgcg cgcgcgcacc ttgcggtaca
    23821 cccacatgcc gtcgtcgcgc tggtaggcgc cggcggcctt cacctcttcg gtgggctcgg
    23881 ccttgtgcac cagctctacg tcgccgtcgg cctcgaccgc ctgcatgaag gggtcggtga
    23941 cgccgaccga gatgttgaag ttggtgagat cgccttcatc cttggcgtgg atgaattcct
    24001 cgatgtcggg gtggtcgcag cgcagcacgc ccatctgcgc gccgcggcgc gcgccggcgg
    24061 actccaccgt ctcgcacgag cggtcgaaca cgcgcatgta ggacaccggg ccggaggcgt
    24121 tggaggccgt gcccttgacc agcgcgccct tgggacggat ggacgagaag tcgtagccga
    24181 cgccgccacc gcggcgcatg gtctcggcgg cctgggcgag cgcggtgtag atgccggggc
    24241 gaccatcgac cgcctcggtg accgagtcgc cgaccggttg cacgaagcag ttgatcagcg
    24301 tggcggcgag cgtggtgccg gcggcgctgt tgatgcgacc ggcgggcacg aagcctttct
    24361 cctgtgcctc gaggaacttc gcctcccagt gggcgcgctt gtcctcggct tcgactgcag
    24421 ccagcgcgcg cgcgacgcgg cgacgcactt cggcgacggt ctgttcgtcg cccttggcgt
    24481 acttctcgac gagcacctcg ccggagattt cctgcatcgg caggttggcg gcggaggctt
    24541 tgacggtgtt ctgggtggac gcgctggtgg tctcgctcat ggtctttttc ttcctccgtg
    24601 gtgggtcttg ggacggacgg aaggatagcg cggaacgagt gctcgtgcaa aaaaataaaa
    24661 cgtttaaaaa acaagtattt atacaatctc tttgtatttc atgattttgc agggcactac
    24721 atgtagtagc ttccgaagtg gggtttttgg gctggaatcc cagtctccat gcgggtttgc
    24781 gccacgctgc ggcccctacc tgcgtgggaa gaacagcgac aggtaggcgg cgaaatcgat
    24841 ctccgcacct tcgcgcaggc ccgccaggcg ctccgcctcg agcccgagca ggtcccaggt
    24901 ctcgggcgcg acgcgggcgg cggcggcgct gctcgggagg tccagctcgt cggcctcggc
    24961 gatgcggcgg gcgaggttga tggttgccgc ctcggcggcg taggcgccgc ccaggcgcgg
    25021 cagcatctgc gcgcccaccg cggtgccgaa gcggtcgggc agttgccagc ggtcgagcag
    25081 ggcggcgccg agttccgcga agtcgcagcc gaccaggcga cgctcggcgg cgtcgagcgc
    25141 ctcgtcgccg gcttgcgccg caaggcgcgc ggcggcggcg agctcgggca cggtctggta
    25201 catcaccagg tggccggtgt ctgcgagcac gccgatcagg aacaggcgtt cgccggcggt
    25261 gaggccggcg gcgatcccgg ccgcgcgcgc ggccagtccg gtgagcaggc tgttgcgcca
    25321 gaagcgccgc atgtcgaggc gggcggggcg gacgccggcg aagatgccct gcagggacat
    25381 cgccagcacc agatcgtgca cctgctgcag gccgagcagc gcgaccgcgc gcacgatgtc
    25441 ctcgacgcgg ccgcgctggc cgaacagggg gctgttgacc aggtgcagca ggcgggccga
    25501 gagcgcgggg tcggcggaga ccaggcgggc gacggtgacg accgagccgg ccggcgactc
    25561 gagttgttcg cgcacgcgca tgtaaacatc gggcagcgag gtcagcgcct cgatctggct
    25621 gaccagttcc tgggcggagt gcattgtcgg ggtcctgtgg atcgggggag gggctcagcc
    25681 ggcatcgcgt gcgcgcggcg tacgtggggt gaagaccagc ggctgcgccg gcggcagctc
    25741 gggttgcagg gtggcgtgct cgatgccgaa gcggctgcgc agcagggcgc gctcggccgc
    25801 cagcaccgtc ggccagcgcg ccgcgtcggc gagcacgaca tgggcggaga gcgcgctgcg
    25861 gtttgaatcc aggctccaga tgtggaggtc gtgcaccgag cgcacgccct cgacgcccgc
    25921 catcgcgtga ccgacttcgg gcagcgacag gccgtcgggc acgccctcga gcagggtgtg
    25981 gaccacctgg cgcagcagcg ccagcgtgga gtacaggatc aggccgcaga tcagcatggt
    26041 gagcagcggg tcgatcggca tccagccggt gtacaggatc acgatgcccg aggcgagcgc
    26101 ggcgaccgag ccgagcaggt cgccgatcac gtgcagcagc gcgccgcggg tgttgagatc
    26161 gccctcgccg cgggtcagca gccaggccac ggccaggttc agcaccagcc cgccgagcgc
    26221 gaccaggatc accgcctcgc cggccaccgc gcgcggctcg agcaggcgct cgaccgcgtg
    26281 ccacaccagg ccgccgacga tcaccagcat gaagagcccg ttggcgagcg cggcgagcgc
    26341 ctcgacccgg cgcaggccgt aggagtggcg ggcgctggcc gggcgcagcg cgatgcgcgc
    26401 cgcgagcgcc gccagcccga gcgaggccgc gtcggtgagc atgtggccgg cgtcgccgag
    26461 cagggcgagc gagcccgacc accatccggc gatcgcctcg accgcggcga agccgagcgt
    26521 gagcacgagc gcgagcggca ggaagcggct cgcggagtgg tcgtgcgcgt gatgaggggg
    26581 gtgggcgtga ccggcttgat gcgggtatgc gtggccgtgt gcggggtcgc cgtgttggtg
    26641 gtcgtgctcg ttgtgggcgt ggtggtcgtg ctgatgaccg tgatgggtgt gcatcgcagc
    26701 gcttcctcga gggcttcagc ggattatgcc tgtggcgcag tcggtcccga cacctccggc
    26761 atccgcggcg ggccgcggcg cgcgtccaca cccttcgggg actcagcgat gcagcagccc
    26821 cgagaagcgc tccaccacct cgacgacctc ggtggcgccg ttgtggatgc cgtcgatgac
    26881 ctcgccggcc tcgcgtgcca agccgatgct cgcggtggcg gtctcgtgca tgccgtgcac
    26941 ccgcgagatc gctaggtggg tggcgtcctg cacggtggag atcatggcgc tgatctcggt
    27001 ggtggccttg gaggtgcgct cggcgagctt gcgcacctcg tcggc
//
LOCUS       DAOQBS010000030        26094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1160_length_26094_cov_17.775299, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000030 DAOQBS010000000
VERSION     DAOQBS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 26094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26094
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1160_length_26094_cov_17.775299"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1628
                     /locus_tag="PKN25_05445"
     CDS             228..1628
                     /locus_tag="PKN25_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HNV90379.1"
                     /translation="MSDQTRTSRPLELDPVDQCPPGGRLFTLALQHVLVMYAGAIAVP
                     LIVGGALKLPKDQIAFLINADLLCCGLVTIIQALGVGRFGIRLPIMMGVTFAAVGPMV
                     AMAGNPALGINAILGAGIAAGLFGILFAPLVARALPLFPPVVTGSIIAVIGLSLMPVA
                     INWSAGGQPRIRDAATGLMVDNPAYGAPEYLAIAAIVLVVVLAITRYGRGFLSNVAVL
                     VGMGVGFAIAIALGKVGFDGLADAPWVSIVTPLHFGAPIFDPISIATMCLVMVVVMVE
                     SLGMFLALGELTGRRLSRDDLTRGLRTDGLGTLIGGFLNTFPHTSFSQNVGLVGVTGV
                     KSRWVCVAGGAILMAFGLFPKMALIIASVPQYVLGGAGLVMFGMVAATGIKILLAADL
                     RNNRFNLYIVALSVGAGMIPMVAPKFFQQMPHALEPLLHSGILLAAITAVTLNLFLNG
                     GRRSASAEAIEPALAH"
     gene            1681..3186
                     /gene="xdhA"
                     /locus_tag="PKN25_05450"
     CDS             1681..3186
                     /gene="xdhA"
                     /locus_tag="PKN25_05450"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HNV90380.1"
                     /translation="MDSQTIRILLDGEAVSITDLAPTTSLLAWLRGARGLVGTKEGCN
                     EGDCGACTVVVGELADDPADAPRLYAVNACIRFLATLDGKAVFTVEHLRSLAGGALHP
                     VQQAMVDCHGSQCGFCTPGFTMSLWALYEDRHAQGEAAADPARIRSALAGNLCRCTGY
                     RPIVEAGERMFDAPSRLLDRDRLRTALLALRRDDTLDFGHEGGRFVAPRSVDELLALR
                     SRHPAATLLAGGTDIGLWVNKGLRRLDTLIHTGEVAALRAIVPHAGGLRIGAAASLTA
                     AFAALVRLHPELAELRERFASPPVRNAGTLGGNVANGSPIGDSMPALIALGARVVLAS
                     VRGRRSLALEDFYLDYMKKDLAADEIVEAIELPPPAPARRLRSWKLAKRFDSDISAVC
                     AGFALELRAGRIHAPRIAFGGMAATPRRATATEAALAGRPWDEAALAAAQCALEHDFA
                     PLDDLRASAGYRRRAAAGLLARFFLETRPDAPLAAAQTRAFAAEDLAGGGR"
     gene            3197..5620
                     /gene="xdhB"
                     /locus_tag="PKN25_05455"
     CDS             3197..5620
                     /gene="xdhB"
                     /locus_tag="PKN25_05455"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HNV90381.1"
                     /translation="MNEPIEPTSSPPASAPPAGQPHPHESAHLHVAGEATYVDDLPEP
                     AGTAHAALGLSTQAHARIVAMDFGAVLAAPGVLGVLVAADIPGRNDCGPIVHDDPILA
                     AGEVHYVGQPIFAVIADERDAARRAARLARIDYAPLPARLDPLEAACAGELVLPPMRL
                     MRGDVDAALAAAPHRVEGRWQVGGQEHFYLEGQVALAQPREDGCMQVWCSTQHPSEMQ
                     QVVAHCLGIAAHRVVVEMRRMGGGFGGKESQSALFACVAALAAARFRRAIKLRPDRDD
                     DIAITGKRHDFHYDYTVGHDEEGRLLALRVDMVARAGCSADLSGPVATRALCHVDNAY
                     HLPAADLRARLARTHTQSNTAFRGFGGPQGAILAEWVIDGIARTLGRDPLDVRRANFY
                     GGPGRDTTPYGQVVADNVLHALVDELEASSDYRARRAEIAAFNAAAPVLRRGLALTPV
                     KFGISFNLTHLNQAGALVHVYADGSVLVNHGGTEMGQGLHTKVCQVVAAELGIALERV
                     RATATDTSKVANTSATAASTGADLNGQAAAAAARTIRMRLAEFALRRFAEGGEGGQGE
                     DRGDARPGRRPADPAAVRFADGQVYVGAHTIPFAELVGAAYRARVQLWSDGFYATPGL
                     HWDAATLTGHPFHYFAYGAAVSEVVVDTLTGEWRLLRADLLHDAGSSINPAIDRGQVE
                     GAFIQGMGWLTMEELWWNAEGRLMTHAPSTYKIPAAHDCPADLRVRLFHNPNRADTLL
                     RSKAVGEPPLLLGFSVFFALRDAIAAAGDGHSNPPLRAPATPEAILDALDAVRRRPTP
                     DARRPTPPI"
     gene            5671..>6002
                     /locus_tag="PKN25_05460"
     CDS             5671..>6002
                     /locus_tag="PKN25_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNV90382.1"
                     /translation="MPSWISTLSCLLETDARAVLVTVAATRGSAPRAAGAHMVVTAAQ
                     VQGSVGGGRLEQRAIGIARELLVADAPSPRLERFALGASLGQCCGGVVVLAFERVGPA
                     QREWVGRAA"
     assembly_gap    6003..6102
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6433..6993
                     /gene="xdhC"
                     /locus_tag="PKN25_05465"
     CDS             6433..6993
                     /gene="xdhC"
                     /locus_tag="PKN25_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HNV90383.1"
                     /translation="MLDASPPPALQVVMFGAGHVGRALAELFGRLPLRVRWVDARAQE
                     FPPALAPNVEPHCTDTPTAEVRAAPAGAVFLVLTHSHALDFELVEAILARDDFRFCGL
                     IGSQTKRARFAHRLHARGIEEARIARLRCPVGLPGLPGKEAEVIALAIAAEVMRLRPQ
                     AHPPSTPRRPAAGAPATQPTESGHET"
     gene            6990..8297
                     /gene="guaD"
                     /locus_tag="PKN25_05470"
     CDS             6990..8297
                     /gene="guaD"
                     /locus_tag="PKN25_05470"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HNV90384.1"
                     /translation="MKPSAPPSPRLSAVRGEIVHFLADPAADPRALEHFADGVLIVRD
                     GHVAECGPAAALLPKLPAGTPLADHRGKLILPGFVDTHVHYPQTDIIASHGEQLLEWL
                     EKYTFPAERRFADRAHAAEVAGFFCDELLRNGTTTAAAFATVHAASVDALFEAARARR
                     MRMITGKVLMDRNCPDFLRDTAETGYAESKALIERWHNRDRLLYAITPRFAPTSTPAQ
                     MTLAGRLFNEHPGVFLQSHLAENRAEVAWVAQLYPQARSYLDVYARAGQLGTRAVFAH
                     CIWLDDADRRHMAETGAAISFCPTSNLFLGSGLFDLRRARALGVRVGLGTDVGGGTSF
                     SMLQTMNEAYKVLQLAGQSLSAASAFHLATLGGAHSLYLDDRIGNLAPGKEADFVVLD
                     PRATPLLERRSAACATLEERLFVLMMLGDDRAVAATHLMGVPA"
     gene            complement(8317..11079)
                     /locus_tag="PKN25_05475"
     CDS             complement(8317..11079)
                     /locus_tag="PKN25_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV90385.1"
                     /translation="MIRTSLNFRLWLTIAIAVLPVLLFVFFDFHERREQAVAETRAEI
                     ALRLADANREAQAAHRAVALVLRIMARSNDLQALDSAQCSGIAGRLLESLDGFANIGA
                     ALPDGEIFCSGRPMSAPVTVIDRAWFRTSLDSDEISPGEFVIGRVSGTPGMAFGYPLR
                     AADGTLRAVIFATITVAWFDRLIEGFRLPEGWEASVISNSGLVLAHQPERAPEGWRAH
                     KVPATTLARMAAVVRDEGGIAELAGIDGVQRLYGIGVPDFAPGSGFFAIGAPLERSLE
                     AAGQRLLMHLALVAAIALASALIARLYIYRLIEVWATRVRGAIAEIAAGRRDARIGQA
                     TGVAELDALSEGIDQMALEIGKREGELQRLSMVIEQSPESIVITDTAGCILYVNEAFQ
                     RITGYARDEVLGRNPRILNGGLTPRATYEQMWATLLAGEVWRGEFHNVRKDGSAYLEL
                     ATIAPIKDAGGTVTHYVAVKEDITQRKQSEALLHRLAYFDALTGLPNRALLHDRIAQA
                     IRSGVRRESFGMLMLVDIDRFRQLNDTLGHAAGDRLLCEVAARLRASVREEDTVARHG
                     DDDFAVLVERIGETEADALSHAEHVARKLQRALQQPYVLGGTEGDRHFATLSFGISLF
                     HDGVSSLESLLKQAEVALYRAKQDGRDIVRFFNPEMQAVVDAHARLEARMHEALEANA
                     FRLFFQPQVNRRGVVIGAEALLRWPLDGGAMVSPAEFIPLAEDTGHIVRLGSWVLRSA
                     CAQLARWQMNETTRHLKIAVNVSARQFHQPDFVASVKDTVRAAGVDPCRLELELTESA
                     ILSDMDETIRRMNELRALGIRFALDDFGTGYSSLSYLKRLPLDQLKIDQSFVRDMAED
                     EGSEAIVLAILSLSHALGLEVVAEGVERPEQRDFLRHHGCDCFQGYLFGKPLPMEAWG
                     DFLEMI"
     gene            11392..12297
                     /locus_tag="PKN25_05480"
     CDS             11392..12297
                     /locus_tag="PKN25_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNV90386.1"
                     /translation="MELKWLEDFLSLADSGSFSRSAEQRHVSQPAFSRRIRALEAWLG
                     VALVDRSTFPTRLTEAGEAFKGPAADLIHRLHAARALVRGHQTTAGDALVFAVPHTLA
                     FAFFPTWLGRLKAEFGEVPTRLIAGNVHDVVTMLVEGGCDLLLCYHHPTHPVWLDPAR
                     FEGIQLAVERVRPYLPRRLARSAGWQLPGSAEAPIPFLRYGPNTYLRRMVDTILDRAA
                     HPAHLALQYESDMAESLKAMLLAGHGLAFLPASAVAREVERGEVVVAGGPEWSLEMEV
                     RLYRDRANTKPELLRLWQHLAAKPL"
     gene            12581..13891
                     /locus_tag="PKN25_05485"
     CDS             12581..13891
                     /locus_tag="PKN25_05485"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNV90387.1"
                     /translation="MSATPQDKAALDRVGGLSYLNPAAPEPWASFVEQIERVRPHLGA
                     LERWIETLKHPKRALIVDVPIERDDGTVAHYEGYRVQHNLSRGPGKGGVRFHPDVTLN
                     EVMALAGWMTIKNAAVGLPFGGAKGGIRVDPASVSKGELQRITRRYTSEIGIIIGPDK
                     DIPAPDVGTNAMTMAIMMDTFSMNRGGTATGVVTGKPIALGGSLGRQEATGRGVFIAA
                     REAARHLRLPIEGARVVVQGFGNVGGIGARMFHDAGARVIAIADHTAILVNEAGIDIP
                     AALEHTAANGGLKGFAGAAPIDPEAFWRLECEFLVPAALEGQLTVERAAGARARIVVE
                     GANGPTTPAADDILHERGILVVPDVLANAGGVTVSYFEWVQDFSSFFWTEDEINERLE
                     RIMVAAFTAIWKVAQEKQVSLRTAAFIIACARVLEARAERGLYP"
     gene            complement(14146..14907)
                     /locus_tag="PKN25_05490"
     CDS             complement(14146..14907)
                     /locus_tag="PKN25_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV90388.1"
                     /translation="MNWHAVRAIYLFEMARTKRTLLQSVLAPVITTSLYFVVFGSAIG
                     ARIPEIEGVSYGAFIVPGLIMLNLLTQSVSNASFGIFFPKFSRTIYEILSAPVSHFEI
                     VLSYVGAAATKAVILSLIILATATFFVPLRIEHPLWMIAFLLLTAMSFSLLGFILGIW
                     ADNFEKLQLVPLLIITPLTFLGGSFYSIDMLPAAWRTVSLFNPVVYLVSGFRWSFYGS
                     ADVAVGVSLAMTLLFLAVFVGVATWMLRTGYRLKP"
     gene            complement(14904..15926)
                     /locus_tag="PKN25_05495"
     CDS             complement(14904..15926)
                     /locus_tag="PKN25_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90389.1"
                     /translation="MDPIISISALSKTYASGFSALKGVDLDIRRGEIFALLGPNGAGK
                     TTLISIVCGLVNASSGSVRVDGHDIVRDYRAARALVGLVPQELTTDAFETVWNTVSFT
                     RGLFGKPPDPAYIEQLLKDLSLWAKKDARLMMLSGGMKRRVLIAKALSHEPRILFLDE
                     PTAGVDVELRRDMWQLVRRLRDSGVTVILTTHYIEEAEEMADRIGVIAKGALILVEDK
                     AVLMTKLGGKQLTLQLAAPLAEVPPALARYGLVLGKGGKTLVYRYEVGRGEGGEGGEG
                     SDPAGDGAIAGLLQDLAAAGIMFTDLHTTQRSLEEIFVSLVSEREAPGTDERGNRAEA
                     AGEGAR"
     gene            16176..17225
                     /locus_tag="PKN25_05500"
     CDS             16176..17225
                     /locus_tag="PKN25_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNV90390.1"
                     /translation="MLRRLSLHRLPWLVLALALSGTACATEVLRVLSWPGYADADVVQ
                     AFEARTGARVEVTQVDSDETLWQKLSTNDATDYDVFAVNTAELQRYIDRGVAVAIDPA
                     ALPNLGAQLPRFRNPATLPGTTRDGKLYAIPYAWAEMGLIYDRRQFDAPPQSIAALWD
                     ARYRGKVLVYNSGSHNFSLAAQMLGKASPFRLDAADWAPAVERLVELRRNLLTFYAQP
                     EESAHLFVSRGAALMYANYGTQQLQLLRAAGADVGYAIPREGALAWLDCWVVTRGARN
                     QALALAWIDHLLGTGPAHVLSARHGLDNTRDPAPHQAETDRLVWLEPVEDVERRNLLW
                     ERILSGDRGARVLAP"
     gene            17222..18919
                     /locus_tag="PKN25_05505"
     CDS             17222..18919
                     /locus_tag="PKN25_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV90391.1"
                     /translation="MRFGIAARLGIVLALSGMLSAGITAFQAQRASETVLVDSARAEL
                     LTSTRVLAQRITQVREDISRTLLALARHPAALAALESDDPQAATQLATLLALPMAANR
                     AYFQLRLIGAADHGLERVRIDRDGDALLRVDGDALQEKGHYPYVFDALALAAGEVYMS
                     RVSINRERGSHAALGRPAVQLATPVVDESGRPRGVIVGSIDLNGVFALLAADLPADFL
                     LYLANSAGDYLIHPDPARTFGFDKGRRVRVQDEFPATQAVIDGRSSESMIDSDAGEHA
                     QAPLVAAFIAARVQLRTEEGAFILGLARPRAAVLAELDPLRGIMLQIVIGMSLTGILL
                     AAILARAITHPIEVLGAAVQNFYDNGQAPALPVERQDEIGALARRFAELRTQIGQQLA
                     ELEHRRRELEHLARHDALTGLPNRALFDDRMAVALASARRDDGGLALLFVDIDDFKPI
                     NDRLGHVVGDRVLEGVAARIRQSIRESDTAARIGGDEFVVLLPGIASAEDARVVADKI
                     RAAIDRPFMIDGGELAVSVSMGLAHYPQDGSDSTTLARHADAAMYRDKAEGKARRHS"
     gene            19079..19687
                     /locus_tag="PKN25_05510"
     CDS             19079..19687
                     /locus_tag="PKN25_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV90392.1"
                     /translation="MIARILCSLLLFCIPFAAHAGAVFQERGDAGDFASPQDVVGTSI
                     ERIQGSIGGDDSIDVFRFHFAGGPLAILARLDGFDDEALPIRLVRESGLPDEACFVGS
                     AEYPPNPCISEGWLDFSVDMLAPGNYLLGVCASAQGCVADDPPFTIGFVTHPDASAPT
                     ATVGRPVPEPGVLALLGLALLGLWGLRMAIAAPPKGPPPSGG"
     gene            complement(19727..20305)
                     /locus_tag="PKN25_05515"
     CDS             complement(19727..20305)
                     /locus_tag="PKN25_05515"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNV90393.1"
                     /translation="MTPTFTPVALEHASRLLNHGPTVLVTSAQGGRRNVMAAAWSMPV
                     EFSPPRIAIVIDKRTFTRELIAASGCFGVCIPGAALADLTFAVGSCSGREGDKFARFG
                     IAALAGPVLGVPLIEAGCAAWMECRLIPERHTEDAYDTCFAEVVAAAADARVFSSGRW
                     DLRADNAELHTLHHLGAGNFARAADVVRARPL"
     gene            20553..23561
                     /locus_tag="PKN25_05520"
     CDS             20553..23561
                     /locus_tag="PKN25_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV90394.1"
                     /translation="MSAPRGGVFERPLVQAAVAVLLVFGLLAAFLLFSHRSAETITTT
                     ALRNEVRVLATQIEGALRRIEMSIGFMRDKYIRIGLHENAGTPAWEASLVQLQLELQH
                     LARGFPEAVAILVTDAQGKILASTLDTPPQWDIGDREYFRHAREHRQDALAFSEPVIY
                     KAGPREVVVAYEAIRNPAGDFVGVVAVALDLQRLQDLLLGVDAGRQGMVSIRRSDDGR
                     LVLRLPDHGASRVADITSTEPFLAIRRGETSGSARYVGIADEVERSFAFQALRDYPFY
                     VVIGRAISEQFAPWRQTATVATIIALATLGLLLALQAALHRSRRQLARSKRSFDALID
                     SRREATCAWRPDTTLLTCNERYAELLGTEAASLPGTRWIDNVPEAKRAEVHAAIARML
                     QGAGTLTTDRRLRQADGRTRWLRWLDLPVLDEEGRCVEVHSIGQDITAQKEVELRLRQ
                     LALAVEQSPNTIVITDTDGRIEYVNEAFVRTTGYTAAEAIGQNPRLLNAGKTPAATYA
                     DLWATLGRGEVWRGEFINTRKDGGTYTELATIAPIKQADGSVSHYVAIKEDITGRREA
                     EARIRQLAYYDTLTGLPNRSLMWDRLRHAIAASARSGASGMLMLLDIDHFKLLNDTQG
                     HEVGDALLREVAQRLRGALREEDTVARVGDDDFAIVIENLGADRDEAIGRAEKIAEHL
                     HRCVTAPCELGLASGPYHVGASLGLTLFRGRNAAADAVLKQAEVAVARAKDDGRNLIR
                     FFSEAMQAVVAARAELELKLRAALAGNGFRLYYQPQLDRNGRVIGAEALIRCFDAEGE
                     MISPAAFIPLAEETGLIVPIGEWVLETACAQLRAWQRTPSTAGLSLSINVSARQFHQP
                     DFVGKVAAAIERHRIRPGGLEIELTESVVIGDIETTVLRMRQIKALGVRFALDDFGTG
                     YSSLSYLKRLPFDQLKIDQMFVRDMEKDTSSEAIVRAILALSRSLDLKVVAEGVETAA
                     QHELLLTRGCELFQGYLFGRPVPIEDWKAG"
     gene            23563..26094
                     /locus_tag="PKN25_05525"
     CDS             23563..26094
                     /locus_tag="PKN25_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HNV90395.1"
                     /translation="MAYVHPTHARAAQRSLGVLGVGLAGVLALWWLPTRWAAGLAHYL
                     PLHLALETASIVAAVLVFAVAWLTPERSAAANTRILGCCFLGVAVLDFTHMLSYLGMP
                     DFITPSSPEKAIDFWIAARLLAALGMLTAVLLPWRVRPGLSPGLLLLAVGAYVGLAHA
                     LFLPRPEWAPRTFIPGQGLTGLKIGIEIGLVLLFAASAALLLRRMRAPCGFDAPALFG
                     AAGLMALSELFFTMYAQVTDLYNLAGHLYKIAAHALLFRALVVDGIRRPYAALERSRH
                     ELADNSARLADERQRLESILAGTRAGTWEWNVQTGATIFNARWAEMLGYRLDELEPLS
                     IDTWRRFAHPDDLADSDAAVRRHLRGETPYYEHETRMRHRAGHWIHVQDRGVLRTHTA
                     DGAPEWMFGTHIDITDRYRAEQERAGALKRLRQLSESVPGVLFECRVEDDGKVSFPYV
                     GRQIEALIGCRAEEAEHDAAAFYARLHPDDGARVRDEIRHARHGMHDWNASFRVVHPL
                     YGERRIRGEARCQPRTDGVGEWFGYLQDETGTHRDQERLRLGARVFDASQEGILVTDA
                     AVRIVDMNPAMEALSGHAKASLLGRNPRELGSGRHDAAFFAEMYASLARDGRWQGEIW
                     NRHSSGEIYVVKASMSTVLDESGRIEHYVGVFTDISTLMAQRDHFSSLANFDALTGLP
                     NRRLLDDRLQQAVGNARRGKPLVVCMLDLDGFKPVNDSVGHAEGDRLLVELAQRMQHI
                     LRAGETLARLGGDEFVLLLVDGDFRPALERVLEVTRAPVALSGGRFARVTASIGASAY
                     RHGVEDGAQLLREADQALYRAKRAGRNRWVMFAPEAAAGVETPPA"
BASE COUNT         3779 a         9799 c         8739 g         3677 t
ORIGIN      
        1 cgacggtttt cgtgggagcg acgcaagtcg cgatgcggcg cccgagagac cgccttcgcc
       61 cttccgccgc aggctcccgc tctccttccc ctgccccacc acgcctcccc ctccccgcac
      121 acaccatacg gatcccctga tccgtcctat aagcccataa gggtttccat atcacggcgc
      181 gatcctagac tggactcatc ggtccataac gacaaggaga cgccacgatg tccgatcaaa
      241 cccgcacgag ccggccgctc gaactcgacc cggtagacca gtgcccgccc ggcgggcgcc
      301 tgttcaccct cgccctgcag cacgtgctgg tgatgtacgc cggcgcgatc gcggtcccgc
      361 tgatcgtcgg cggcgcgctc aagctgccca aggatcagat cgcctttctc atcaacgccg
      421 acctgctgtg ttgcggcctg gtcaccatca tccaggcgct cggcgtcggc cgcttcggca
      481 tccgcctgcc gatcatgatg ggcgtcacct tcgccgcggt cgggccgatg gtggcgatgg
      541 cgggcaaccc cgcgctcggc atcaacgcca tcctcggcgc gggcatcgcc gccggcctct
      601 tcggcatcct gttcgcgccc ctggtggcgc gcgcgctgcc gctgttcccg cccgtggtca
      661 ccggctcgat catcgcggtg atcggcctct cgctgatgcc ggtggcgatc aactggtcgg
      721 ccggcggcca gccgcgcatc cgcgacgcgg cgaccggact gatggtggac aaccccgcct
      781 acggtgcccc ggaatacctc gccatcgccg ccatcgtgct ggtcgtggtg ctggcgatca
      841 cccgctacgg tcgtggcttc ctgtccaacg tggcggtgct ggtcggcatg ggcgtgggct
      901 tcgcgatcgc gatcgcgctc ggcaaggtcg gcttcgacgg cctcgccgac gcgccctggg
      961 tgtcgatcgt gacgccgctg cacttcggcg cgccgatctt cgacccgatc tcgatcgcca
     1021 ccatgtgcct ggtgatggtg gtcgtgatgg tcgagtcgct cggcatgttc ctcgcgctcg
     1081 gcgagctcac cggccgccgg ctgtcgcgcg acgacctcac ccgcggcctg cgcaccgacg
     1141 gcctcggcac cctgatcggc ggcttcctga acaccttccc gcacaccagc ttctcgcaga
     1201 acgtcggcct ggtcggcgtc accggcgtca agagccgctg ggtgtgcgtg gcgggcggcg
     1261 cgatcctgat ggccttcggc ctcttcccca agatggcgct gatcatcgcc tcggtgccgc
     1321 agtacgtgct cggcggcgcc ggcctggtga tgttcggcat ggtcgccgcc accggcatca
     1381 agatcctgct cgcggccgac ctgcgcaaca accgcttcaa cctctacatc gtcgcgctgt
     1441 cggtgggcgc cggcatgatc ccgatggtgg cgccgaagtt cttccagcag atgccgcacg
     1501 cgctcgaacc cctgctgcac agcggcatcc tgctcgcggc gatcaccgcg gtgacgctca
     1561 acctcttcct caacggcggc cgccgcagcg ccagcgccga ggcgatcgag ccggcgctgg
     1621 cgcactgagg cggcgccgaa cgcgctacca tcttcgggcc tgcgcccccg ggatccgaag
     1681 atggacagcc agacgatacg catcctgctc gacggcgagg cggtgtcgat caccgacctc
     1741 gcccccacca ccagcctgct cgcctggctg cgcggcgcgc gcggcctcgt cggcaccaag
     1801 gaaggctgca acgagggcga ctgcggcgcc tgcaccgtgg tggtgggcga gctcgccgac
     1861 gaccccgcgg acgcgccgcg cctgtacgcg gtcaacgcct gcatccgctt cctggccacg
     1921 ctcgacggca aggcggtgtt caccgtcgaa cacctgcgca gcctcgccgg cggcgccctg
     1981 cacccggtgc agcaggcgat ggtcgattgc cacggctcgc aatgcggctt ctgcaccccc
     2041 ggcttcacga tgtcgctgtg ggcgctgtac gaggaccgtc acgcgcaggg cgaggccgcc
     2101 gcggatcccg cccgcatccg cagcgcgctc gccggcaacc tgtgccgctg cacgggctat
     2161 cggccgatcg tggaggccgg cgagcgcatg ttcgacgccc cgtcgcgcct gctcgaccgc
     2221 gatcgcctgc gcaccgccct gctcgcgctg cgccgcgacg acacgctcga tttcggccac
     2281 gagggcggcc gcttcgtcgc cccgcgcagc gtggacgaac tcctcgccct gcgcagccgc
     2341 caccccgcgg ccaccctgct cgccggcggc accgacatcg gcctgtgggt caacaagggc
     2401 ctgcgccggc tcgacaccct gatccacacc ggcgaggtcg ccgccctgcg cgcgatcgtg
     2461 ccgcacgccg gcggcctgcg catcggcgca gcggcttcgc tcacggccgc cttcgccgcg
     2521 ctcgtccgcc tgcacccgga gctggccgag ctgcgggagc gcttcgcctc gccgccggtg
     2581 cgcaacgccg gcacgctcgg cggcaacgtc gccaacggct cgccgatcgg cgactcgatg
     2641 cccgcgctga tcgcgctcgg cgcgcgcgtg gtgctcgcga gcgtgcgcgg ccggcgcagc
     2701 ctggcgctgg aggacttcta tctcgactac atgaagaagg acctcgccgc cgacgagatc
     2761 gtcgaggcga tcgaactgcc gccgcccgca cccgcgcgac gcttgcgcag ctggaagctg
     2821 gccaagcgct tcgactccga catctccgcg gtatgcgccg gcttcgcgct cgagctgcgc
     2881 gcgggccgga tccacgctcc gcgcatcgcc ttcggcggca tggcggcgac gcccaggcgc
     2941 gccaccgcca ccgaggccgc gctcgccggc cggccctggg acgaagccgc gctcgccgcc
     3001 gcacagtgcg cgctcgaaca cgacttcgcc ccgctcgacg acctgcgcgc gagcgccggc
     3061 taccgccgcc gcgccgccgc cggcctgctc gcgcgcttct tcctcgagac ccgccccgac
     3121 gcgccgctcg ccgcggcgca gacgcgcgcc ttcgccgcgg aagatctcgc cggcggcggg
     3181 cgctgaggag aacgtgatga acgagccgat cgagcccacc tcgtcaccgc cggcatccgc
     3241 cccccccgcc ggccagccgc atccgcacga gtccgcccac ctgcacgtgg ccggcgaggc
     3301 cacctacgtc gacgacctcc ccgaacccgc cggcaccgca cacgcggcgc tcggcctgtc
     3361 gacgcaggcg cacgcgcgca tcgtggcgat ggacttcggc gcggtgctcg cggcgcccgg
     3421 cgtgctcggc gtgctggtgg ccgccgacat tccgggacgc aacgactgcg ggccgatcgt
     3481 gcacgacgac cccatcctcg ccgcaggcga agtgcactac gtcggccagc cgatcttcgc
     3541 ggtgatcgcc gacgagcgcg acgccgcgcg ccgcgccgcg cgcctcgccc gcatcgacta
     3601 cgcaccgctg ccggcgcggc tcgacccgct ggaggccgca tgcgcgggcg agctcgtgct
     3661 gccgccgatg cgcctcatgc gtggcgacgt cgatgcggcg ctggccgcgg cgccgcatcg
     3721 cgtggagggg cgatggcagg tcggcggcca ggagcacttc tacctcgaag gccaggtcgc
     3781 gctcgcccag ccgcgcgagg acggctgcat gcaggtgtgg tgttccaccc agcacccgag
     3841 cgagatgcag caggtggtgg cgcactgcct gggcatcgcc gcccaccgcg tggtcgtgga
     3901 gatgcgccgc atgggcggcg gcttcggcgg caaggagtcg cagtcggcgc tgttcgcctg
     3961 cgtggcggcg ctggcggcgg cgagatttcg ccgtgcgatc aagctgcgcc ccgatcgcga
     4021 cgacgacatc gccatcaccg gcaagcgcca cgacttccac tacgactaca ccgtcggtca
     4081 cgacgaagag ggccgcctcc tcgcgctgcg cgtcgacatg gtcgcgcgcg cgggctgctc
     4141 cgccgacctc agcggcccgg tcgccacgcg cgcgctctgc catgtcgaca acgcctacca
     4201 cctgccggcg gccgacctcc gtgcccggct cgcgcgcacc cacacccagt ccaacaccgc
     4261 cttccgcggc ttcggcggac cgcagggcgc gatcctcgcc gagtgggtga tcgacggcat
     4321 cgcgcgcacg ctcgggcgcg atcccctcga cgtgcgccgc gccaacttct acggcggccc
     4381 ggggcgcgac accacgccct acggccaggt ggtcgccgac aacgtgctgc acgcgctggt
     4441 cgacgaactc gaagcgagca gcgactaccg cgcccgccgc gccgagatcg ccgccttcaa
     4501 cgccgccgct ccggtgctgc ggcgcgggct ggcgctcacc ccggtgaagt tcggcatctc
     4561 cttcaacctc acccacctga accaggccgg cgcgctggtg catgtgtatg ccgacggctc
     4621 ggtgctggta aaccacggcg gtaccgagat gggccagggc ctgcacacca aggtctgcca
     4681 ggtggtcgcc gccgagctcg gcatcgcgct cgagcgcgtg cgcgccaccg ccaccgacac
     4741 cagcaaggtg gccaacacct cggccaccgc ggcctccacc ggcgccgacc tcaacggcca
     4801 ggccgcggcc gccgccgcgc gcacgatccg catgcgcctg gcggagttcg cactgcgccg
     4861 cttcgccgag gggggcgagg ggggccaagg cgaagaccgc ggcgatgcgc gcccaggccg
     4921 ccggcccgcc gacccggccg cggtgcgctt cgccgacggc caggtgtacg tcggtgcaca
     4981 cacgatcccc ttcgccgagc tcgtcggcgc ggcctaccgc gcccgcgtgc agctgtggtc
     5041 cgacggcttc tacgccacgc ccggactgca ctgggacgcg gccacgctca ccggccaccc
     5101 cttccattac ttcgcctacg gcgcggcggt gtccgaggtg gtggtcgaca cgctcaccgg
     5161 cgaatggcgc ctgctgcgcg ccgacctgct gcacgacgcc ggcagctcga tcaacccggc
     5221 gatcgaccgc ggccaggtcg agggcgcctt catccagggc atgggctggc tcaccatgga
     5281 agagctgtgg tggaacgccg agggccgcct gatgacccac gcgccttcca cctacaagat
     5341 ccccgccgcg cacgactgcc cggcggacct gcgcgtgcgc ctgttccaca accccaaccg
     5401 cgccgacacc ctcctgcgct ccaaggcggt cggcgaaccg ccgctgctgc tcggtttctc
     5461 ggtcttcttc gcgctgcgcg acgccatcgc cgccgccggc gacggccaca gcaacccgcc
     5521 cctgcgcgca cccgccaccc ccgaggccat cctcgacgcg ctcgacgcgg tgcgccggcg
     5581 cccgacgccc gacgcccgac gcccgacgcc tcccatctga cgcctgacgc ctgacccccc
     5641 gacgtttcgt ccttcgcagc tcacctcccc ttgccctcct ggatctccac cctctcctgt
     5701 ctgctcgaga ccgacgcccg cgcggtcctg gtcacggtcg ccgccacacg aggttcggca
     5761 ccgcgcgccg cgggtgcgca catggtggtg acggccgccc aggtgcaggg cagcgtcggc
     5821 ggcggccggc tggagcagcg cgccatcggg atcgcgcgcg agctgctcgt cgccgacgcc
     5881 ccttccccgc gcctcgaacg tttcgccctc ggcgccagcc tcggccagtg ctgcggcggc
     5941 gtcgtggtgc tggccttcga gcgcgtcggc ccggcacagc gggagtgggt cgggcgcgcc
     6001 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaggagcga cgcgagtcgc
     6121 gatcgggccg acgagggggc gacggcgccg ggatccgccg cgccccccgc cccgagcgcc
     6181 ggatcgcgac ttgcgtcgct cctacaggaa gacctcgccg ctcctcgtag gagcgacgcg
     6241 agtcgcgatc gggccgacga gggggcgacg gcgccgggat ccgccgcgcc ccccgccccg
     6301 agcgccggat cgcgacttgc gtcgctccta caggaagacc tcgccgctcc tcgtaggagc
     6361 gacgcgagtc gcgatcgggc cgacggatcg gcgacagcgc ccggatccgc cgcggcgctc
     6421 gggacggcga tcctgctcga tgcgagcccg ccgccggcgc tgcaggtggt gatgttcggc
     6481 gcgggccatg tggggcgggc gctggcggag ctgttcgggc ggctgccgct gcgcgtgcgc
     6541 tgggtcgacg cgcgcgcgca ggaattcccg cccgcgcttg cgcccaatgt cgaaccgcac
     6601 tgcaccgaca cgccgacggc ggaggtgcgc gcggcgcccg cgggcgcggt cttcctggtc
     6661 ctcacccaca gccacgcgct cgacttcgag ctggtcgaag ccatcctcgc gcgcgacgac
     6721 ttccgctttt gcggcctgat cggctcgcag accaagcgcg cgcgcttcgc ccaccgcctg
     6781 cacgcgcgcg gaatcgaaga agcgcgcatc gcacgcctgc gctgcccggt cggcctgccc
     6841 ggcctgccgg gcaaggaagc cgaggtgatc gcgctcgcga tcgccgccga agtgatgcgc
     6901 ctgcgcccgc aagcgcatcc cccgagcacg ccgcgccggc ccgcagcggg cgcaccggcc
     6961 acgcaaccca cggaatccgg ccacgagaca tgaagccctc cgccccaccc tccccgcggc
     7021 tctccgccgt acgcggcgag atcgtccact tcctcgccga ccccgcggcc gacccgcgcg
     7081 cgctcgagca cttcgccgac ggcgtgctga tcgtgcgcga cggccacgtc gccgaatgcg
     7141 gccccgccgc cgcgctgttg cccaagctgc ccgccggcac gccgctcgcc gaccaccgcg
     7201 gcaagctgat cctgcccggc ttcgtcgaca cccacgtgca ctacccgcag accgacatca
     7261 tcgccagcca cggcgagcag ctgctcgaat ggctggagaa atacaccttc cccgccgagc
     7321 gccgcttcgc cgaccgcgcg catgccgccg aggtcgccgg cttcttctgc gacgagctgc
     7381 tgcgcaacgg caccaccacc gcggcggcct tcgccacggt gcacgcggcc tcggtggatg
     7441 cgctcttcga ggccgcgcgc gcgcgccgca tgcgcatgat caccggcaag gtgctgatgg
     7501 accgcaactg ccccgacttc ctgcgcgaca cggcggagac cggctacgcg gagtcgaagg
     7561 ccttgatcga gcgctggcac aaccgcgacc gcctgctgta cgccatcacc ccgcgcttcg
     7621 cgcccacctc cacgcccgcg cagatgacgc tcgccggtcg cctcttcaac gagcacccgg
     7681 gcgtcttcct gcagtcgcac ctcgccgaga accgcgccga ggtggcctgg gtggcgcagc
     7741 tgtacccgca ggcgcgcagc tacctcgacg tgtatgcccg cgccggccag ctcggcacgc
     7801 gcgcggtgtt cgcgcactgc atctggctcg acgacgccga ccgccggcac atggccgaga
     7861 ccggcgcggc gatcagcttc tgccccacct ccaacctctt cctcggctcg gggctcttcg
     7921 acctgcggcg cgcgcgcgcg ctcggcgtgc gcgtgggact gggcacggac gtggggggcg
     7981 gcaccagctt ctcgatgctg cagaccatga acgaggccta caaggtgctg cagctcgccg
     8041 gccagtcgct gtcggcggcg agcgctttcc acctcgccac cctgggcggc gcgcacagcc
     8101 tctacctcga cgaccgcatc ggcaacctcg cgcccggcaa ggaggccgac ttcgtcgtcc
     8161 tcgacccgcg cgccacgccc ctgctcgagc gccgcagcgc cgcctgcgcg acgttggaag
     8221 agcgcctctt cgtgctgatg atgctcggcg acgaccgcgc ggtggcggcc acgcacttga
     8281 tgggcgtgcc ggcctgaggc ccgcgcgcgg ccggcctcag atcatctcga ggaagtcgcc
     8341 ccaggcctcc atcggcagcg gcttgccgaa caggtagccc tggaagcagt cgcatccgtg
     8401 gtggcgcagg aagtcgcgct gctccggcct ctccactcct tcggccacca cctccaggcc
     8461 gagcgcgtgg ctcagcgaca ggatcgccag cacgatcgcc tcgctgccct cgtcctcggc
     8521 catgtcgcgc acgaaggact ggtcgatctt caactggtcc aagggcaggc gcttgaggta
     8581 ggacagcgag gagtagccgg tgccgaagtc gtcgagcgcg aagcggatgc cgagcgcgcg
     8641 cagctcgttc atgcgccgga tggtctcgtc catgtccgac aggatggcgc tctcggtgag
     8701 ttccagctcc aggcggcagg ggtcgacacc cgcggcgcgc acggtgtcct tcacgctggc
     8761 gacgaagtcg ggctggtgga actggcgcgc gctgacgttg accgcgatct tgaggtggcg
     8821 ggtggtctcg ttcatctgcc agcgcgcgag ctgggcgcag gcgctgcgca gcacccacga
     8881 gccgaggcgg acgatgtggc cggtgtcctc ggcgagcggg atgaactcgg ccggcgacac
     8941 catggcgcca ccgtcgagcg gccagcgcag cagggcctcg gcgccgatca ccacgccgcg
     9001 ccggttgacc tggggctgga agaagagccg gaaggcgttc gcttccagcg cctcgtgcat
     9061 gcgggcctcg agacgggcgt gggcatcgac caccgcctgc atctcggggt tgaagaagcg
     9121 cacgatgtcg cgcccgtcct gcttggcccg gtacaaggcc acctcggcct gcttgagcag
     9181 gctctcgagc gagctcacgc cgtcgtggaa caggctgatg ccgaagctca gcgtcgcgaa
     9241 gtggcggtcg ccctccgtac caccgagcac atagggttgt tgcagcgcgc gctggagctt
     9301 gcgtgcgacg tgctcggcgt ggctcagcgc atcggcctcg gtttcgccga tgcgctcgac
     9361 gagcacggcg aagtcgtcgt cgccatggcg cgcgaccgtg tcctcctcgc gcacgctcgc
     9421 gcgcaggcgc gccgcgacct cgcacagcag gcggtctccg gcggcgtgac cgagggtgtc
     9481 gttgagctgg cggaagcgat cgatgtcgac cagcatcagc atgccgaagc tctcgcggcg
     9541 cacaccgctg cggatggcct gggcgatgcg gtcgtgcagc agcgcgcggt tgggcagccc
     9601 ggtcagcgca tcgaagtagg ccagccggtg cagcagcgcc tcggattgct tgcgctgcgt
     9661 gatgtcctcc ttgaccgcca cgtagtgggt gaccgtgccg cccgcgtcct tgatcggcgc
     9721 gatcgtcgcc agctcgagat aggcggagcc gtccttgcgg acgttgtgga actcgccgcg
     9781 ccacacctcg cctgcaagca aggtcgccca catctgctcg taggtcgcgc gcggcgtcaa
     9841 cccgccgttg aggatgcgcg ggttgcgtcc gagcacctcg tcgcgcgcat agcccgtgat
     9901 gcgctggaag gcctcgttca catacaggat gcaccccgcg gtgtcggtga tgacgatcga
     9961 ctccgggctc tgctcgatca ccatcgacag gcgttgcagc tcgccctcgc gcttgccgat
    10021 ctccaacgcc atctggtcga tgccctcgga gagcgcgtcg agctcggcaa cgccggtggc
    10081 ctgcccgatg cgcgcatcgc gccgcccggc ggcgatctcg gcaatcgcac cgcgcacccg
    10141 cgtcgcccac acctcgatca gccggtagat gtacaagcgc gcgatcagcg cggaggccag
    10201 cgcgatggcc gcgaccaggg ccaggtgcat cagcaggcgc tgccccgccg cctccagcga
    10261 gcgctccagc ggcgcaccga tcgcgaaaaa gccgctgcca ggggcgaaat ccggcacgcc
    10321 gatgccgtac aggcgctgca cgccatcgat gccggcgagt tcggcgatgc cgccttcgtc
    10381 gcgcacgacg gcggccatgc gcgcgagcgt cgtcgcgggg accttgtgcg cgcgccagcc
    10441 ctcgggcgca cgctcgggct ggtgcgcgag caccaggccg gaattgctga tcacgctcgc
    10501 ctcccaaccc tcaggcaggc ggaacccttc gatcaggcga tcgaaccacg ccaccgtgat
    10561 ggtggcgaag atcaccgcac gcagcgtgcc gtccgccgca cgcagcgggt agccgaaagc
    10621 catgcccggc gttcccgaca ccctgccgat gacaaactcc ccgggggaaa tttcatcact
    10681 gtcgagagaa gtgcggaacc aggcgcgatc gatcaccgtc accggggcgc tcatcggcct
    10741 cccgctgcag aagatctccc cgtcgggcag cgcggcgccg atgtttgcga aaccgtccag
    10801 cgactcgagc aggcgcccgg cgatgcccga gcattgcgcg gaatccagcg cttgcaggtc
    10861 gttggagcgc gccatgatcc gcaacaccaa cgcgaccgcg cgatgcgcgg cctgcgcctc
    10921 gcggttcgca tccgccaggc gcagcgcgat ctcggcgcgg gtctcggcca ccgcctgttc
    10981 acggcgctcg tggaagtcga aaaagacgaa cagcagcacc ggaagtaccg cgatcgcgat
    11041 cgtcagccac aggcggaaat tgagcgaggt acggatcatc ggtcgtcccg cgacgggcgt
    11101 cgacgggggc tctccggcgc atgggggatt gggggacagt gcgcgaactc tgccgcaatt
    11161 ttgcggcgca gtcgcgcccg gttacctgaa tcatgcgaac cagttcacga aacgccccat
    11221 tatggggcct gcgaattccg aatgcatgcc cgggcatgtc ggccgcgcgc gtcctgcccc
    11281 ggcccggcga gcaccgggcc cgatgggcga cgggcgccga gcggcggtac gcggggaacg
    11341 ccggcggatt tcctgcatac aatggggtct ctccccctgc agggcccgcc catggaactc
    11401 aaatggctgg aagatttcct cagcctcgcc gacagcggga gcttctcgcg ctcggccgag
    11461 cagcgccacg tctcgcagcc cgccttctcg cgccggatcc gcgcgctcga ggcctggctg
    11521 ggggtggcgc tggtcgaccg cagcaccttc ccgacccggc tcaccgaagc gggcgaggcc
    11581 ttcaagggac cggcggcgga tctcatccac cgcctgcatg cggcgcgcgc gctggtgcgc
    11641 ggccaccaga cgaccgcggg cgacgcgctc gtgttcgccg tcccgcacac gctcgccttc
    11701 gccttcttcc cgacctggct ggggcggctc aaggccgagt tcggcgaggt ccccacccgg
    11761 ctgatcgcgg gcaacgtgca tgacgtggtg accatgctgg tggaaggcgg ctgcgatctg
    11821 ctgctgtgct accaccatcc gacccacccg gtgtggctcg atcccgcgcg cttcgaaggc
    11881 atccagctcg cggtcgagcg cgtgcgcccc tacctgccgc gccggctcgc gcgcagcgcg
    11941 ggctggcagc tgcccggttc ggccgaggcg cccatcccct tcctgcgcta cggacccaac
    12001 acctacctgc gccgcatggt cgacaccatc ctcgaccgcg ccgcccatcc ggcccacctc
    12061 gccctgcagt acgagtccga catggccgag agcctcaagg ccatgctgct cgccggtcac
    12121 ggcctcgcct tcctgcccgc gagcgcggtc gcgcgcgagg tggaacgcgg cgaggtggtg
    12181 gtcgccggcg gcccggagtg gagcctggag atggaggtcc gcctctaccg cgaccgcgcc
    12241 aacaccaagc ccgagctgct gcgcctgtgg cagcacctcg ccgcaaagcc tctgtaggaa
    12301 ccgctgcagg agcgacgcaa gtcgcgattg cgccgcgtcg gaccacgtcg gatgtcggtc
    12361 gcggccaagc caccgcaatc gcgactcgcg tcgctcctgc gccagaaccc gagcgccgcg
    12421 ggtagccgag gcacgcggct gcacaccgac tcaccggccc ctccggcccc tccggccacc
    12481 ctatgcgcaa gacgcatgac ccgatgtagc aacggcatgc cttcgggcca gggcaatcca
    12541 cacaatgcgc gcaccttctc acaccacacg aggcagacag atgagtgcga caccacaaga
    12601 caaggcggcc ctcgaccgcg tcggcggcct ttcctacctg aacccggccg cccccgagcc
    12661 gtgggcgagc ttcgtcgagc agatcgagcg cgtgcgcccc catctcggcg cgctcgaacg
    12721 ctggatcgag accctcaagc atcccaagcg cgcgctgatc gtcgatgtgc cgatcgagcg
    12781 cgacgacggc accgtcgccc actacgaggg ctaccgcgtg cagcacaacc tgtcgcgcgg
    12841 cccgggcaag ggcggcgtgc gcttccaccc cgacgtcacc ctcaacgagg tgatggcgct
    12901 ggccggctgg atgacgatca agaacgccgc ggtcggcctg cccttcggcg gcgccaaggg
    12961 cggcatccgc gtcgatccgg cgagcgtcag caagggcgag ctgcagcgca tcacccgccg
    13021 ctacacctcc gagatcggca tcatcatcgg cccggacaag gacatccccg cgcccgacgt
    13081 cggcaccaac gcgatgacga tggcgatcat gatggacacc ttctcgatga accgcggcgg
    13141 caccgccacc ggcgtcgtca ccggcaagcc gatcgcgctc ggcggcagcc tgggccgcca
    13201 ggaagccacc ggccgcggcg tcttcatcgc cgcgcgcgag gccgcccgcc acctgcgcct
    13261 gccgatcgag ggcgcgcgcg tggtcgtgca gggcttcggc aacgtcggcg gcatcggcgc
    13321 gcgcatgttc cacgacgccg gcgcgcgcgt gatcgcgatc gccgaccata ccgccatcct
    13381 ggtgaacgag gccggcatcg acatccccgc cgcgctcgag cacaccgccg cgaacggcgg
    13441 cctgaagggc ttcgccggcg ccgcgccgat cgaccccgag gcgttctggc gcctcgagtg
    13501 cgagtttctc gtccctgccg cgctcgaagg ccagctcacc gtcgagcgtg ctgccggcgc
    13561 gcgtgcgcgc atcgtcgtcg agggcgccaa cggcccgacc accccggccg cggacgacat
    13621 cctccacgag cgcggcatcc tggtcgtgcc cgacgtgctc gccaacgccg gcggcgtcac
    13681 cgtgtcgtac ttcgaatggg tgcaggactt ctccagcttc ttctggaccg aggacgaaat
    13741 caacgagcgc ctcgagcgca tcatggtcgc cgccttcacc gcgatctgga aggtggccca
    13801 ggagaagcag gtgtcgctgc gcaccgccgc cttcatcatc gcctgcgcgc gggtgctgga
    13861 ggcgcgcgcg gagcgcgggc tgtacccgta aggggcgagg cgccgggcct tgcgccgcgg
    13921 cacctccgtc gcaagcgatt gcagtggtgg cgatccgtca ccgctgcaat cgcgacttgc
    13981 gtcgctccca cgggagaagc acggacccgc ggcggtagac ggaatcgccg cggtgaccgg
    14041 aaaacgcacc ggtccggaat ctcccgtggg agcgacgcaa gtcgcgattc cgacgcccgg
    14101 gatgcggcac cccggatgcg gcgcccgtcg gcgggcctgc gcccctcaag gcttcagccg
    14161 ataccccgtc ctcaacatcc aggtcgccac ccccacgaac accgccagga acagcagcgt
    14221 catcgccagg ctcaccccca ccgccacatc cgcgctgccg tagaagctcc agcggaagcc
    14281 gctcaccagg taaaccacgg ggttgaacag cgacaccgtg cgccacgccg ccggcagcat
    14341 gtcgatcgag tagaagctgc cgccgaggaa ggtcagcggg gtgatgatca gcagcggcac
    14401 gagttgcagc ttctcgaagt tgtccgccca gatgccgagg atgaagccga gcaggctgaa
    14461 gctcatcgcg gtgagcagca ggaaggcgat catccacagc ggatgctcga tgcgcagcgg
    14521 cacgaagaag gtggcggtgg cgaggatgat caggctcagg atcaccgcct tggtcgccgc
    14581 cgcgcccacg tagctcagca cgatctcgaa gtgcgacacc ggcgccgaca ggatctcgta
    14641 gatcgtgcgc gagaacttcg ggaagaagat gccgaaggag gcgttcgaca cgctctgggt
    14701 gagcaggttg agcatgatca ggccgggcac gatgaaggcg ccgtagctca cgccctcgat
    14761 ctcggggatg cgcgcgccga tcgccgagcc gaagacgacg aaatacagcg aggtggtgat
    14821 caccggcgcc agcacgctct gcagcagcgt gcgcttcgtg cgcgccatct cgaacaggta
    14881 gatcgcgcgg accgcgtgcc agttcatcgt gcgccctccc ctgcggcctc ggcccggttg
    14941 ccccgctcgt ccgtacccgg cgcttcgcgc tcgctcacca ggctgacgaa gatctcttcg
    15001 agcgaacgct gggtggtgtg caggtcggtg aacatgatcc ccgcggcggc gaggtcctgc
    15061 agcaagcctg cgatcgcgcc atcgcctgcc ggatcgctgc cctcgccccc ttccccgcct
    15121 tcgcccctgc cgacctcgta gcgatagacc agcgttttgc cgcccttgcc cagcaccagc
    15181 ccgtagcgcg cgagcgcggg cgggacctcg gcgagcggcg ccgccagttg cagcgtgagc
    15241 tgcttgccgc cgagcttcgt catcagcacg gccttgtcct cgaccaggat cagcgcaccc
    15301 ttggcgatca cgccgatgcg gtcggccatc tcctccgcct cctcgatgta gtgcgtggtc
    15361 aggatcacgg tgacgccgct gtcgcgcaag cggcgcacga gctgccacat gtcgcggcgc
    15421 agctccacgt ccaccccggc ggtgggctcg tcgaggaaga ggatgcgcgg ctcgtgcgag
    15481 agcgccttgg cgatcagcac gcggcgcttc atgccgcccg agagcatcat cagcctggca
    15541 tccttcttcg cccacagcga caagtccttc agcagctgct cgatgtaggc cgggtcgggc
    15601 ggcttgccga acaggccgcg cgtgaagctc accgtgttcc acaccgtctc gaaggcgtcg
    15661 gtggtgagtt cctgcggcac caggccgacg agggcgcgcg cggcgcggta gtcgcgcacg
    15721 atgtcgtgac catccacccg cacgctaccc gagctggcat tgaccaggcc gcacacgatg
    15781 ctgatcagcg tggtcttgcc cgcgccgttg ggcccgagca gcgcgaagat ctcgccgcgg
    15841 cggatgtcga ggtcgacacc cttcagcgcg gagaagccgg aggcgtaggt cttggacaga
    15901 gcggagatcg agatgatggg gtccatggga tcgaagataa cagatcgacg aggcggcccg
    15961 caggatcgag cggcaccggg cacgatgagc ggcagcggag tgccccaagg ccgcgatcgc
    16021 gacttgcggc gctccagcat gggcggcccg tccaccgccc ggcggcggct ggcggccagc
    16081 cgtcgggcgc gcttccgcgg caggctcggc gcgcttaaac tgcctcgcac cccccaagat
    16141 ccttccccgc cgccagatcc aggagccgat ccgtcatgct tcgtcgcttg agcctgcatc
    16201 gcctcccctg gcttgtgctc gcgctcgcac tctccggcac ggcctgcgcg accgaggtgt
    16261 tgcgcgtgct gagctggccg ggctatgccg acgccgacgt ggtgcaggcc ttcgaggccc
    16321 gcaccggcgc ccgggtcgaa gtcacccagg tcgattccga cgaaacgctg tggcagaagc
    16381 tcagcacgaa cgacgcgacc gactacgacg tgttcgcggt caataccgcc gagctgcagc
    16441 gctacatcga ccgcggcgtg gccgtggcga tcgatccggc cgcgctgccc aacctcggtg
    16501 cgcaattgcc gcgcttccgc aacccggcaa cgctgcccgg cacgactcgc gatggcaagc
    16561 tgtacgccat cccctatgcc tgggccgaaa tgggcctgat ctacgatcgg cgccagttcg
    16621 acgcgccacc ccagtccatc gccgcgctgt gggacgcccg ctaccgcggc aaggtgctgg
    16681 tgtacaacag cggctcgcac aacttctcgc tcgccgcaca gatgctgggc aaggcatcgc
    16741 ccttccgcct cgacgccgcc gactgggcgc cggcggtcga gcgtctggtc gagttgcgcc
    16801 gcaacctgct gaccttctac gcccagccgg aagaatccgc gcatctgttc gtcagccgcg
    16861 gcgcggcgct gatgtacgcc aattacggca cccagcagct gcagctcctg cgcgcagcgg
    16921 gggcggacgt gggctatgcg atcccgcgcg aaggcgcgct cgcctggctc gactgctggg
    16981 tggtgacgcg cggcgcacgt aaccaggcgc tcgcgctggc gtggatcgac cacctgctcg
    17041 gcaccggccc ggcgcacgtg ctgagcgcgc gccacggcct cgacaacacc cgcgacccgg
    17101 cgccgcacca ggccgaaacc gaccgcctgg tctggctcga accggtcgag gacgtcgaac
    17161 gccgcaacct gctgtgggag cgcatcctct ccggcgaccg cggcgcacgg gtgctcgcgc
    17221 catgagattc ggcatcgccg cccgtcttgg catcgtactc gccctgtccg gcatgctctc
    17281 cgccggcatc accgccttcc aggcccagcg cgccagcgag accgtgctgg tggacagcgc
    17341 gcgtgccgag ctgctcacct ccacccgggt gctcgcgcag cgcatcacgc aggtgcgcga
    17401 ggacatctcg cgcaccctgc tcgcgctcgc ccgccacccg gccgcgctcg cggcgctgga
    17461 gagcgacgac ccgcaggctg cgacgcagct cgcgaccctg ctcgccctgc cgatggcggc
    17521 caaccgcgcc tacttccagc tccgcctgat cggcgccgcc gaccacggcc tcgagcgcgt
    17581 gcggatcgac cgcgacggcg acgcactgct gcgcgtggac ggcgacgcgc tgcaggaaaa
    17641 gggccactac ccctacgtct tcgacgccct cgcgctggca gcgggcgagg tttacatgtc
    17701 gcgcgtgagc atcaatcgcg agcgtggctc gcacgccgcc ctcggccggc ccgcggtgca
    17761 gctcgccacc ccggtcgtcg acgaatcggg ccggccacgc ggggtgatcg tgggcagcat
    17821 cgacctcaac ggcgtgttcg ccctgctcgc cgccgacctg cccgccgact tcctgctcta
    17881 cctcgccaac agcgcgggcg actacctgat ccaccccgac cccgcgcgca ccttcggctt
    17941 cgacaaggga cgccgggtac gggtgcagga cgagttcccc gccacgcagg ccgtgatcga
    18001 cggccgcagc agcgagtcga tgatcgacag cgatgccggc gagcacgcgc aggcgccgct
    18061 ggtcgccgcc ttcatcgccg cgcgggtgca gctgcgcacc gaagagggcg ccttcatcct
    18121 cggcctcgcg cgcccacgcg ccgccgtgct cgccgagctc gatccgctgc gcgggatcat
    18181 gctgcagatc gtcatcggca tgagcctcac cggcatcctg ctcgcggcca tcctcgcccg
    18241 cgcgatcacc catccgatcg aggtgctggg tgccgcggtg cagaacttct acgacaacgg
    18301 ccaggccccg gcgctgccgg tcgagcgtca ggacgagatc ggcgccctcg cccgccgctt
    18361 cgccgagctg cgcacccaga tcgggcagca gttggccgag ctcgaacacc gccgccgcga
    18421 gctcgagcac ctcgcccgcc acgacgcgct caccgggctg cccaaccgcg cgctcttcga
    18481 cgaccgcatg gcggtggcgc tcgcgtccgc ccgccgcgac gacggcgggc tggccttgct
    18541 gttcgtcgat atcgatgatt tcaagccgat caacgaccgc ctcggccacg tcgtcggcga
    18601 ccgcgtgctc gaaggcgtgg cggcgcgcat ccgccagagc atccgcgagt ccgacaccgc
    18661 ggcgcgcatc ggcggcgacg agttcgtcgt gctgctgccc gggatcgcgt cggccgaaga
    18721 cgccagagtg gtcgcggaca agatccgcgc ggcgatcgac cgcccgttca tgatcgacgg
    18781 tggagagctc gcggtttcgg tgagcatggg cctcgcccac tacccgcagg acggcagcga
    18841 ctccaccacc ctcgcccgcc acgccgatgc cgcgatgtac cgcgacaagg ccgaaggcaa
    18901 ggcacggcgg cacagctgaa accggccgcc agccggcatg gaagtgtcgg aaatttttac
    18961 acccggtgcc gaaccttcaa aaataagcgc agataatcaa tggtctgatc gtcgggcacg
    19021 gcgaatgctt ccccccaagg gagggcagta cccgacactc cggagcaagg aggccagcat
    19081 gatcgcgcgc atcctctgca gcctgctgtt gttctgcatc ccgttcgccg cgcacgccgg
    19141 cgcggtgttc caggaacgcg gcgacgccgg tgatttcgcc tcgccgcagg acgtggtcgg
    19201 aacctccatc gaacgcatcc agggcagcat cggcggcgac gacagcatcg acgtgttccg
    19261 cttccacttc gccggcggcc cgcttgcgat cctcgcccgc ctcgacggct tcgacgacga
    19321 agccctgccg atccgcctgg tgcgcgagag cggcctgccc gacgaggcct gctttgtggg
    19381 cagcgccgag tatccgccca acccgtgcat ctccgagggc tggctggact tctccgtcga
    19441 catgctggcg ccgggcaact acctgctggg tgtttgtgcg tccgcacaag gctgcgtggc
    19501 ggacgatccg cccttcacga tcggcttcgt gacccacccc gatgcgagcg cacctacggc
    19561 caccgtcggc aggcccgtgc cggagcccgg cgtgctcgcg ctcctcggcc tcgccttgct
    19621 gggcctgtgg ggactgcgca tggcgatcgc cgcaccgccg aagggaccgc ccccttccgg
    19681 cgggtaaaga ccggtttcgt ccggccgatc gcgcctgccg gcgcgcctac agagggcgtg
    19741 cgcggacgac gtcggcggcg cgggcgaagt tgccggcgcc gaggtggtgg agcgtatgca
    19801 gttcggcgtt gtcggcgcgc aggtcccaac gcccggacga gaacacccgc gcgtcggcgg
    19861 ccgcggccac gacctcggcg aagcaggtgt cgtaggcgtc ctcggtgtgg cgctcgggga
    19921 tcaggcggca ctccatccac gccgcgcagc cggcctcgat cagcggcacg cccagcacgg
    19981 gccccgcgag cgccgcgatg ccgaagcgcg cgaacttgtc gccctcgcgg ccactgcaac
    20041 tgcccaccgc aaacgtcagg tcggcgagcg ccgcgcccgg gatgcacact ccgaaacagc
    20101 cactggcggc gatcagttcg cgggtgaagg tgcgcttgtc gatcacgatg gcgatgcgcg
    20161 gcggagagaa ctcgaccggc atcgaccacg ccgccgccat cacgttgcgc cgacccccct
    20221 gggcgctggt gacgagcacc gtgggtccgt ggttgaggag ccggctggcg tgttcgaggg
    20281 cgacgggggt gaaggtgggg gtcatggtgg cttccgcgga ggatcgaaga ggccgccatt
    20341 ttccatgaat cgcggaccgc gccgtgcgtg tcccgctcgc atgccgggcg gatttgcgtc
    20401 gcccgtcgtg acatagtgca gtgacctgtc tgctgctgtc cggataattg cggcttgttc
    20461 cctgtccgat tgctccccat gtccgattcc gcgcgccgct tcctcgtccc gcaccgggcg
    20521 ccgacgcgca gcacgcgtgc gcagggggtc cgatgagcgc cccgcgcggc ggcgtcttcg
    20581 agcgtccgct ggtccaggcg gcggtggcgg tgctgctggt gttcgggctg ctcgccgcct
    20641 tcctgctgtt ctcccaccgc tcggccgaga cgatcaccac caccgccttg cgcaacgagg
    20701 tgcgcgtgct ggccacgcag atcgagggcg cgctgcgccg catcgagatg agcatcgggt
    20761 tcatgcgcga caagtacatc cgcatcggcc tgcacgagaa cgcgggcacc cccgcgtggg
    20821 aggcctcgct ggtgcagctg cagctggagc tgcagcatct ggcgcgcggc tttccggaag
    20881 ccgtcgccat cctggtgacc gacgcgcagg gcaagatcct ggccagcacc ctcgacacgc
    20941 cgccgcaatg ggacatcggc gaccgcgaat atttccgcca cgcccgcgaa caccgccagg
    21001 acgcgctcgc cttctccgag cccgtgatct acaaggccgg cccgcgcgag gtcgtggtgg
    21061 cctacgaagc gatccgcaac ccggcgggcg atttcgtcgg cgtggtcgcg gtcgcgctcg
    21121 acctgcagcg cctgcaggac ctgctgctcg gggtggacgc cggtcgccag ggcatggtca
    21181 gcatccggcg cagcgacgac ggccgcctgg tgctgcgctt gccggaccac ggcgccagcc
    21241 gggtcgccga catcaccagc accgagcctt tcctggccat ccgccgcggc gagacgtccg
    21301 gcagcgcgcg ctacgtcggc atcgcggacg aagtcgagcg cagcttcgcc ttccaggcgc
    21361 tgcgcgacta ccccttctac gtggtgatcg gccgcgcgat ctccgagcaa ttcgcgccct
    21421 ggcggcagac cgcgaccgtc gccacgatca tcgccttggc caccctgggc ctgctgctgg
    21481 cgctgcaggc cgccctgcac cgcagccgcc gccagctcgc gcgcagcaag cgcagcttcg
    21541 acgccctcat cgacagccgc cgggaggcga cctgcgcctg gcggcccgat accacgctgc
    21601 tgacctgcaa cgagcgctac gccgaactgc tcggcaccga ggccgcgtcc ctgccgggca
    21661 cgcgctggat cgacaacgtg ccggaggcga agcgcgcgga ggtccacgcc gccatcgcgc
    21721 ggatgctgca gggcgccggc acgctcacca ccgatcgccg cctccgccag gccgacggcc
    21781 gcacgcgctg gctgcgctgg ctcgacctgc cggtgctcga cgaggagggg cgctgcgtcg
    21841 aagtgcactc gatcggccag gacatcacgg cgcagaagga ggtcgagctg cgcctgcgcc
    21901 agctcgcact ggccgtcgag cagagcccga acacgatcgt gatcaccgac accgatggtc
    21961 gcatcgagta tgtcaacgag gccttcgtgc gcaccaccgg ctacaccgcc gcagaggcga
    22021 tcggccagaa cccgcgcctg ctcaacgccg gcaagacccc cgccgccacc tatgccgacc
    22081 tgtgggccac gctcggccgc ggcgaggtgt ggcgcggcga gttcatcaac acccgcaagg
    22141 acggcggcac ctacaccgag ctcgccacga tcgcgccgat caagcaggcc gacggcagcg
    22201 tcagccacta tgtggcgatc aaggaggaca tcaccgggcg ccgcgaggcc gaggcgcgca
    22261 tccgccagct cgcctactac gacaccctca ccggcctgcc caaccgcagc ctgatgtggg
    22321 accgcctgcg ccacgccatc gcggcgagcg cgcgcagcgg cgcgagcggc atgctgatgc
    22381 tgctcgacat cgaccacttc aagctgctca acgacaccca gggccacgag gtgggcgatg
    22441 ccctgctgcg cgaggtggcg cagcgcctgc gcggcgcgct gcgcgaggag gacacggtgg
    22501 cgcgggtggg cgacgacgac ttcgcgatcg tgatcgagaa cctgggcgcg gaccgcgacg
    22561 aagcgatcgg ccgcgccgag aagatcgccg agcacctgca ccgctgcgtg accgcgccct
    22621 gcgagctcgg cctcgcgagc ggcccctacc acgtcggcgc gagcctgggc ctgaccctgt
    22681 tccgcggccg caacgcggcg gccgacgcgg tgctcaagca ggccgaggtg gcggtggcgc
    22741 gcgccaagga cgacggccgc aacctgatcc gcttcttcag cgaggcgatg caggcggtgg
    22801 tggccgcgcg cgccgagctg gagctgaagc tgcgcgcggc gctggccggc aacggcttcc
    22861 gcctgtacta ccagccgcaa ctcgaccgca acggccgcgt gatcggtgcc gaggcgctga
    22921 tccgctgctt cgacgccgag ggcgagatga tctcgcccgc cgccttcatc ccgctcgccg
    22981 aggagaccgg cctcatcgtg ccgatcggcg agtgggtgct ggagaccgcc tgtgcgcaac
    23041 tgcgtgcatg gcagcgcacg ccctccacgg ccgggctgag cctgtcgatc aacgtcagcg
    23101 cgcgccagtt ccaccagccc gacttcgtcg gcaaggtggc ggcggcgatc gagcgccacc
    23161 gcatccgccc cggcggcctc gagatcgagc tcaccgagag cgtggtgatc ggcgacatcg
    23221 agaccacggt gctgcgcatg cgccagatca aggccctggg ggtgaggttc gcactcgacg
    23281 acttcggcac cggctattcc tcgctgtcct atctcaagcg cctgcccttc gaccagctca
    23341 agatcgacca gatgttcgtg cgcgacatgg agaaggacac cagcagcgag gcgatcgtgc
    23401 gcgcgatcct cgcgctcagc cgctcgctcg acctgaaggt ggtggccgag ggtgtcgaga
    23461 ccgccgccca gcacgagctg ctgctgacgc gcggctgcga gctgttccag ggctatctgt
    23521 tcggccggcc ggttccgatc gaggactgga aggcaggctg agatggcgta cgtccacccg
    23581 acccacgccc gcgccgcgca gcgcagcctc ggcgtgctcg gcgtcggcct cgccggcgtg
    23641 ctcgcgctgt ggtggctgcc cacgcgctgg gccgccgggc tggcgcacta cctgccgctg
    23701 cacctggcgc tggagaccgc gtcgatcgtc gccgccgtgc tggtgttcgc ggtcgcatgg
    23761 ctgacgcccg agcgcagcgc ggcggccaac acccgcatcc tgggctgctg cttcctcggc
    23821 gtggcggtgc tggatttcac tcacatgctg tcctacctcg gcatgccgga cttcatcacg
    23881 ccgagcagcc ccgagaaggc gatcgatttc tggatcgccg cgcgcctgct ggccgcgctc
    23941 ggcatgctga cggcggtgct gctgccgtgg cgggtgcgcc ccggcctgtc tcccggcctg
    24001 ctgctgctcg cggtgggcgc ctacgtgggc ctggcgcacg cgctcttcct gccgcggccc
    24061 gagtgggcgc cgcgcacctt catcccgggc caggggctga cggggctgaa gatcggcatc
    24121 gagatcggcc tggtgctgct gttcgccgca tccgccgcct tgctgctgcg ccgcatgcgc
    24181 gcgccctgcg gcttcgacgc ccccgccctg ttcggtgcgg ccgggctgat ggcgctgagc
    24241 gagctcttct tcacgatgta cgcgcaggtc accgacctgt acaacctcgc cggccatctc
    24301 tacaagatcg ccgcgcacgc cctcctcttc cgcgcgctgg tggtggacgg catccgccgc
    24361 ccctatgccg cgctcgaacg ctcgcggcac gaactcgcgg acaactccgc gcgcctcgcc
    24421 gacgaacgcc agcgcctgga gtccatcctc gcgggcacgc gcgccggcac ctgggaatgg
    24481 aacgtgcaga ccggggcgac catcttcaac gcgcgctggg cggagatgct cggctatcgc
    24541 ctcgacgagc tcgaaccgct gtcgatcgac acctggcggc gcttcgccca cccggacgac
    24601 ctcgccgact cggatgccgc ggtgcgccgt cacctgcgcg gcgagacgcc gtattacgag
    24661 cacgagaccc gcatgcgcca ccgcgccggg cactggatcc acgtgcagga tcgcggcgtg
    24721 ctgcgcaccc acaccgccga cggcgcgccg gaatggatgt tcggcacgca tatcgacatc
    24781 accgaccgct accgcgccga gcaggagcgc gccggggcgc tgaagcgcct gcgtcaactc
    24841 tcggagagcg tgcccggcgt gctcttcgag tgtcgcgtgg aagacgacgg caaggtgagc
    24901 ttcccctacg tcggccgcca gatcgaggcg ctgatcggct gccgcgcaga agaggcggag
    24961 catgacgccg cagccttcta cgcccgcctg catcccgacg acggcgcacg ggtgcgggac
    25021 gagatccgcc atgcgcgcca cggcatgcac gactggaacg cctccttccg cgtggtgcac
    25081 ccgctgtacg gcgaacgccg gatccgcggc gaggcgcgct gccagccgcg gactgacggc
    25141 gtcggcgagt ggttcggcta tctccaggac gagaccggga cgcaccgcga ccaggagcgc
    25201 ctgcgcctgg gcgcacgcgt cttcgacgcc agccaggagg gcatcctggt caccgacgcc
    25261 gcggtgcgca tcgtggatat gaatccggcg atggaggcgc tcagcgggca tgccaaggcg
    25321 tccctgctcg ggcgcaaccc gcgcgagctc ggctcgggcc gacacgacgc ggccttcttc
    25381 gccgagatgt acgccagcct ggcgcgcgac gggcgctggc agggcgagat ctggaaccgc
    25441 cattcatcgg gcgagatcta cgtggtgaag gcgtcgatgt ccaccgtgct cgacgagtcc
    25501 ggccgcatcg agcattacgt cggcgtgttc accgacatca gcacgctgat ggcgcagcgc
    25561 gaccacttca gctcgctcgc caacttcgac gccctcaccg gcctgcccaa ccgccgcctg
    25621 ctcgacgacc gcctgcagca agcggtgggc aatgcgcgcc gcggcaagcc gctggtcgtg
    25681 tgcatgctcg acctcgacgg cttcaagccg gtcaacgaca gcgtcggcca cgccgagggc
    25741 gaccgcctgc tggtcgagct cgcgcagcgc atgcagcaca tcctgcgcgc gggcgagacc
    25801 ttggcccgcc tcggcggcga cgagttcgtc ctgctgctgg tggacggcga cttccgcccg
    25861 gcactcgagc gcgtgctcga ggtgacgcgc gcgccggtgg cgctctccgg cgggcgcttc
    25921 gcccgcgtca ccgcgagcat cggcgcgagc gcgtaccggc acggcgtcga ggatggcgca
    25981 cagctgctgc gcgaggccga ccaggcgctc taccgcgcca agcgcgccgg gcgcaaccgc
    26041 tgggtgatgt tcgcgccgga agctgcagcc ggggtggaga ccccgccggc ctag
//
LOCUS       DAOQBS010000031        25843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1177_length_25843_cov_14.720684, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000031 DAOQBS010000000
VERSION     DAOQBS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 25843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25843
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1177_length_25843_cov_14.720684"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..186
                     /locus_tag="PKN25_05530"
     tRNA            111..186
                     /locus_tag="PKN25_05530"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Asn,seq:gtt)
     gene            complement(469..825)
                     /gene="mnhG"
                     /locus_tag="PKN25_05535"
     CDS             complement(469..825)
                     /gene="mnhG"
                     /locus_tag="PKN25_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HNV90396.1"
                     /translation="MSGAESLPWWAAIPVALLLVLGGIVTLVGTLGLARLQSFYQRIH
                     GPAISIALGAGCILLASMLYFTVMQSRLVIHELLISVFVLMTAPVVSMLIMRAAVYRD
                     LRAAREMDPGNDAGNS"
     gene            complement(905..1189)
                     /locus_tag="PKN25_05540"
     CDS             complement(905..1189)
                     /locus_tag="PKN25_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HNV90397.1"
                     /translation="MTTATLLSAATLFALACVVLAMLLCTLRLMIGPSAQDRVLALDT
                     LWMCVMVLALLLGIRHGSQIYFEAALIIALLGFVSTIALAKFLMRGEVIE"
     gene            complement(1186..1704)
                     /locus_tag="PKN25_05545"
     CDS             complement(1186..1704)
                     /locus_tag="PKN25_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNV90398.1"
                     /translation="MRRWLPSPLLSASLLLMWLMLNQSLEPAHWLLGGALGVLAPLLS
                     QPLQPHGHARIRRPLALFRLLWMAAIEIVRSCFNVTQIILLRRSADVNSQFIRVPLDL
                     KSPHGLALLSCLINSTPGTVWVEILPDGHELLLHVFDLHDEAWWVHTIKTRYEQPIIE
                     VFETPEPGGGRT"
     gene            complement(1701..3326)
                     /locus_tag="PKN25_05550"
     CDS             complement(1701..3326)
                     /locus_tag="PKN25_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HNV90399.1"
                     /translation="MTVLPWLNHLIIVPVLLPLIVGALMIPINQKRHGLKFGLGLASG
                     MLLWAVAVALLVLADGGYWPGGIGVYLAANWAAPFGIALVADRLSALMLVLTATIALA
                     VLVFSARRWDRVGVSFHPLFQFLLMGLNGAFLTNDLFNLFVFFEVMLAASYGLVLHGY
                     NLRRIQAGMQYIAVNLVASLVFLIGVSLIYAASGTLNIADLGARVALLGAQDAWLLQV
                     GAAVLALAFLTKGAMWPLGFWLPTTYASASAPVGAMLVLMTKVGVYAVLRVWLAVFGE
                     GAGAAEGFGFAALTLGGMATVLFGAIGMLASQDGGRMAGYGAIVSSGTLLAVIGHSGG
                     AVLASGLYYLLGSTLAMAAFMLLIELTERIRPPGAALIAITMEAFAIEDKPEDPVGVG
                     IPGTLAFLGLSFAACALVIAGMPPLAGFVAKFSLFHAMLAAAPEGVPATTWGLIGLMI
                     LAGLAAIIALMRLGVRTFWAAGAVTPPRLQFSEAVPVGALVILCVLLTVQAGTVFDYL
                     DRTTAGLLRSGDYAARVLGEPPVLRAPGAEGVR"
     gene            complement(3323..3664)
                     /locus_tag="PKN25_05555"
     CDS             complement(3323..3664)
                     /locus_tag="PKN25_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HNV90400.1"
                     /translation="MELVLAISVGVVFGAGIWLILRPRTFQLITGLLLMSYAVNLFIF
                     AMGRLWVDKPPITPTPAIDPAQFADPLPQGLVLTAIVIGFATTALYLVMMIGARGLSG
                     SDHVDGEEPRE"
     gene            complement(3664..6573)
                     /locus_tag="PKN25_05560"
     CDS             complement(3664..6573)
                     /locus_tag="PKN25_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HNV90401.1"
                     /translation="MLLASLVLLPFLGSVLAALMPQDARNRESWLAIGVAVGGLAIAA
                     ALFPQVAAGEVVRAEFAWLPAEGLDFVLRVDGLAWVFSMLVLAIGLLVVLYARYYMSP
                     EDPVPRFFAFLLAFMGSMLGVVLSGNLVQLVVFWELTSLTSFLLIGYWHHRADARRGA
                     RMAFTVTATGGLCLMAGVLVLGHIVGSYDLDVVLAAGDQIRDHELYLPALVLVALGAL
                     TKSAQFPFHFWLPHAMAAPTPVSSYLHSATMVKAGVFLLVRLWPALAGTPEWTWIIGG
                     AGVCTLLIGAFVAIFQHDLKGLLAYSTISHLGLITVLIGIGTPLAIVAAIFHILNHAT
                     FKASLFMAAGIIDHETGTRDLRVLRGLRHKLPITATLAIVASAAMAGVPLLNGFLSKE
                     MFFAETLHVGGDRNWWMSYAAVAMGMFSVAYSLRFISVFFGTPAQGLPRDPHEPPRWM
                     RFPVELLVLACLVVGVAPGVSIEPLLHLAVVATLGEAAPAYDLAVWHGFNLPLLMSVC
                     ALVGGMLLYGVLRRHFDLARRERVPLLHRLNGAQAYETTMLQLTRGAEWLLRRVGTRR
                     LQPQLMLMMLALLLIPLLLAGAPPAWTVIPVRGFDPIFAGMWLIGAGCALAAAWMAKY
                     HRLAALALVGGTGIVTAATFLWLSAPDLALTQLMVETVTTVLILLGLRWLPPRIESIA
                     GPVQPPWQVWTRRTRDLALAVGGGLALAALTHAVLVHPASDTIADFFLLNALGGGGGS
                     NVVNVLLVDFRSFDTLGEITVLAVVALTVYALLRRFRPAPESAGIPPQQRFEGDPASR
                     QSPAEQAGSGYLLVPGIYLRLLLPFMGVIAVYFFMRGHNLPGGGFVAGLIFAVAILVQ
                     YMLAGTVWVESRLNLRPHRWLGFGLLLACATGLGAWWFGHPFLTTHTAHVEWPLVGEL
                     HLPSAFVFDLGVFAVVVGTTMLMLVALAHQSLRAHRHPGEDLTADGQGGAEGRRI"
     assembly_gap    6831..6930
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7086..7655)
                     /locus_tag="PKN25_05565"
     CDS             complement(7086..7655)
                     /locus_tag="PKN25_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNV90402.1"
                     /translation="MKHRPLMLAASVLALLAQVGPVAAADAPSAPVEPIAALDVPRYM
                     GSWYEIARYPNWFQKKCVGDSRADYSLLESGEVRVVNRCREADGSVSEAVGVARQIGG
                     ADSPRLEVRFAPAWLSFLPIVWGDYWVLDLDPDYRLVAVGEPSREYLWILSRTPTVEA
                     RAYAELLERLAARGFDVGRLVRGEQGGGG"
     gene            7875..8489
                     /locus_tag="PKN25_05570"
     CDS             7875..8489
                     /locus_tag="PKN25_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNV90403.1"
                     /translation="MKLRLALLILVLAALLASGVAPYDRGVWWAEVAPVLIALPILLA
                     SAQRFPLTPLTYGLIAFFSLILILGGAYTYARVPVGFVVQDWFELARNPYDRFGHFFQ
                     GVTPAILGRELLLRTSPLRPGKWLFALLTLSCTGISALYELVEWASAELWAGGAVEFL
                     GTQGDPWDAQADMLMALLGAIIAQIVFGRLHDRQLARLAAQPST"
     gene            8629..10011
                     /locus_tag="PKN25_05575"
     CDS             8629..10011
                     /locus_tag="PKN25_05575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNV90404.1"
                     /translation="MNQPSSNPRRRFLTAAGGLATAAVAGTALAQSAPPAASTERSLP
                     FLIGRATDTLLPPGKQRRVVICGGGWGGVTAAKYLRQLAPQLDVVLLERNPVFFSCPM
                     SNKWLVDQVDTRYLTHDYLAVAEKYAYRFIQTEILEIEREGKRVITAAGAIHYDYLIV
                     APGIRYNYEAWFGNDRRMAEATRARFPAAYIPSAEHFHLKNALQSFKGGDLVMTLPPP
                     PQRCPPSPYERACLIASMFKQKKIPGRIYILDHKDGARPIGPGFRSTFEELYKDIITY
                     VPDARIEEVDPFKRRIRTEAGDFDFQHAILMAPHQAGDLAWKAGLVPRPGAGNPSGGW
                     ADVDPLFLHDRTDKEVFVIGDAVGFVSPQFGFYPKAGHVANRHARIVAYYISEREAGR
                     EPDYRLPDNLCFMMVNIAPQEGISVQFDYKVNAQGIIEQTQIDYNERTAATISQDFGW
                     AQTIYEDMFS"
     gene            complement(10034..12463)
                     /locus_tag="PKN25_05580"
     CDS             complement(10034..12463)
                     /locus_tag="PKN25_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV90405.1"
                     /translation="MADLVPLPATALRKRCDPACFSFDTTESLADHAGDLGQERAVEA
                     IRFGLAMGHTGYHVFVLGDAGSGKHATTFRLLRERAAREDVPPDLCYLHNFDEPLKPR
                     LLTLPAGRGAALRTRMQTFIRDLGPALGAALDSDAHVERVESLQSAHKAREEGALREL
                     GKACNAEGISLLQTPEGFVFAPTKDGEPLSPEAFEALPEDERKAIEARIGAWSDRLED
                     LLNDFPGWRKSVRESIDRAEREVLGPAVAHLLREVREAHEDLPEVLAFLDAVERDVID
                     SAVRGTMLEEEDDEAPEPEENTRYHRYQVKLLVDHSGSQGAPVVFENNPGYGNLIGRI
                     EHIVQQGNQVSHFNLIRAGALHRANGGYLVLDAERLLSQPFAWEGLKRSLRAGEIRIE
                     PPAEAQGWSGALTLEPEAVPSALKVVLIGDREVYYLLMEHDPEFADLFKVAADFNDDI
                     LRTPENECEYARLMALLARGAKLLPVERSGVARLVEEASRLAEDSGRLSLNTRALSDL
                     MREADFHARQKGLAATTRAEVEDALAARARRANRYPTRVLESILDGTTLISTTGERAG
                     QINGLVVIELAGELFGHPVRITATVRMGEGDVVDIERETELGGAIHSKGVLILSAFLA
                     ARYARHQPLSLSASLVFEQSYAPVEGDSASLAELCALLSALTQVPIQQRFAITGSVNQ
                     FGEVQVIGGVNEKIEGFFDLCSARGLDGTQGVVIPAASVRHLMLREDVVQAAAEGRFH
                     IHAVKTVDEAMTVLTGVEAGEPDAKGVIPKGTLNHKIATVLAEMTAARHAYSDADGAA
                     GARQHRRRHGV"
     gene            12718..13797
                     /gene="moaA"
                     /locus_tag="PKN25_05585"
     CDS             12718..13797
                     /gene="moaA"
                     /locus_tag="PKN25_05585"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNV90406.1"
                     /translation="MKVIPIQQAASPLAPAAPAPVAAPGAALVDRRARSVRDLRISVT
                     DRCNFRCVYCMPREVFGDDYAFLPRRQLLSFEEILRVARLFVARGVRKIRITGGEPLL
                     RKDVDRLIGMLAALDGVEVTLTTNGVLLPKLARRLKDAGLHRVTVSLDALDDALFRRM
                     NDADYPVEKVLEGIEAAKVVGLGPIKVNMVVKRGTNDVDIEPMAAHFRNSGHILRFIE
                     FMDVGASNGWKMDEVLPSREIVERIDRLFPLEPVDPNYEGEVAERWRYKDGAGEIGVI
                     SSVTQAFCSSCTRIRLSTEGQLYTCLFAQQGHDLRALLREGADDARIDAAIAQVWQGR
                     EDRYSEIRTAETAALRKIEMSYIGG"
     gene            complement(13847..14464)
                     /locus_tag="PKN25_05590"
     CDS             complement(13847..14464)
                     /locus_tag="PKN25_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNV90407.1"
                     /translation="MESRKRFPRMLAAAVLAGVLGAAPAWAEPRSYTIDPEHFAIGFQ
                     VEHLGYADIIGLFLKASGRFEYDEAARTLKSGRVVVAADSVFSNHEARDRHVRDSDFL
                     DARKHPEIVFEASGFEAGAERDGRLHGKLAGRLTLLGQTHPVVLDVTLNKAASYPFGH
                     RKHTLGISARTTIERSRWGMDYGVSRGMVGDAVALRFELEAMRDD"
     gene            complement(14507..15334)
                     /locus_tag="PKN25_05595"
     CDS             complement(14507..15334)
                     /locus_tag="PKN25_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNV90408.1"
                     /translation="MVAGRCSHCRRAAKREWGLNEVGKGRKGSMAMEQGGSCVRRGGL
                     PRLWLDLAGGFGGLVALVALASSTLALPLAAAWAAGAVLLAIAALIRHFWSDPRRGLG
                     PANRVTLVRALLVALLAGMLAAPAWVDAHPVQVAALAALALLLDGVDGWVARRWRCES
                     DFGARFDMELDAFLILVLCVHLLAMDKAGPWVLAIGAMRYVFVAAMRPWPWLERPLRE
                     SRRRKLVCVWQVACLLLCLLPAIGGALATALLVLALALLAWSFAVDVRWLYHTRPQS"
     gene            15385..16383
                     /locus_tag="PKN25_05600"
     CDS             15385..16383
                     /locus_tag="PKN25_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HNV90409.1"
                     /translation="MSSTNLAFWTVRPGYGELRPAPLRPPADGELRVRNLFGAVSRGS
                     ESLVFRGEVPESEYERMRAPFQEGDFPGPLKYGYIGVGVVEDGVGTAAAALRGRTVFC
                     LHPHQQRYVVPAGAVVPLPAGVPAARAVLAANLETAINACWDGAPALGDRIAVVGAGV
                     VGSLVAWLCARLPGVELELIDTDPGRAGLAAALGLVHRLPEQARGNCDLVFHASGNPA
                     GLVRALELAGQDATVVEMSWYGRRSAELPLGAAFHARRLRLQSSQVGRLPPPRSPRWD
                     YRRRMELALALLVDPRLDALISGETDFTDLPALMQRLAEAPAGALCERIRYASPST"
     gene            16414..16812
                     /locus_tag="PKN25_05605"
     CDS             16414..16812
                     /locus_tag="PKN25_05605"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNV90410.1"
                     /translation="MYSLTVREHIMIAHSFRGEIFGPAQRVHGATYLVDACFQRSELD
                     ADGLVVDIGLAAATLKAVLAEFNFRNLDELEEFRGRNTTTEFMARVIFDRLAATIRAG
                     RLGPGAQGLERLKVSLHESHVAWASYEGAL"
     gene            16809..17882
                     /locus_tag="PKN25_05610"
     CDS             16809..17882
                     /locus_tag="PKN25_05610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV90411.1"
                     /translation="MSAHRGSTDLDFIVPGDPAQRTGGYLYDARIVEALRHLGWTVTV
                     HGLPGRFPDADPTARDALHDTLATLPAGRRVVIDGLALGGLPEVAIEHAGRLALVALV
                     HHPLADERGLDPVLRRCLLASERAALAAVRLAITTSAFTARRLLDFGLRAERIRWVEP
                     GVAPLALAAAEGEPPQLLCVASLTPRKGQDVLVRALERVRALPWRCTLIGSTHRDPGY
                     AGEVAELVRSLGLQDRIRLSGECADAALRDAYAAADLFVLPSHYEGYGMVVAEAIAAG
                     LPVLATTGGALAGTLPPGAGLAVPPGDVDALAGALGELIGDRARRLRLRDGARRARAG
                     LRGWPQAGEAFAAALAELAPASA"
     gene            17912..18868
                     /locus_tag="PKN25_05615"
     CDS             17912..18868
                     /locus_tag="PKN25_05615"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV90412.1"
                     /translation="MPTHPDAVDAPRHPDACFDADWLALRATADRAARAPRLEALAAA
                     WLQGRRRRHPQAPLRLMDLGCGSGANARHLAPRLPGPQQWTLVDHDTGLLERARTLGA
                     DLRDAQGAKPQVDTRCRDLGTLAAPALADTDLVCASAVLDLMPASWLVRLADACTSTG
                     CALLITLSVDGTWRFLTHGSAEPPVPDDAFVRDAFNAHQRRDKGLGAALGPDAAPALA
                     ALLAARGFDVETAASPWRLDLAAPPQAALACALIDGWRDAATAQCPHAAARIAAWHAR
                     RSAQCAARGKEVLEVGHLDLFASPPVATPAALRPDGPAARAR"
     gene            18865..19845
                     /locus_tag="PKN25_05620"
     CDS             18865..19845
                     /locus_tag="PKN25_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNV90413.1"
                     /translation="MSGEARRTRRPLPVLRALASLGLLAGVLWWIEPRALIAAFGAPA
                     PAWLALALAIGVLQTALSAWRWQLTAGRLGAPLGFAHALREYYLASFLNQILPGGVMG
                     DAARAWRHASLPGIARDAAWQAVVIERGAGQLALLLVVVASVLAAPALQVVPGRLGDA
                     VDLRGLPWMGVLAVLALVATALAGRTRTALRSLASATRQALLGRAVLLRQLLASLLIV
                     ASYVAVWLCCMRMSGLATPPAQAAALVPWVLLAMAIPLSVAGWGIREGAAALVWQAAG
                     LDAAEGVAASVSYGVVVLLSTLPGALALRRHEPGARSTAEATPTCRADGP"
     gene            complement(19820..20632)
                     /locus_tag="PKN25_05625"
     CDS             complement(19820..20632)
                     /locus_tag="PKN25_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNV90414.1"
                     /translation="MQRLDLCRLVADVVPDEAPPNPAEQYEALVLVRGHAFLRSSEAA
                     IELEPGQILLGTGASPELLYSADSELWSVLVPESVLREACSECGWLHAGEGVRFNRSP
                     CTRDELDHLVTLLRLVASESASGALRAQLFMHYAHAVANKLLFTLQHNQPTSQTCGQA
                     RLFERLERHIEARIKQDISVEQLAQFAGLSQRSLYQVFKDQARMSPRTYIRRKKLEHV
                     YATLMDPAVRVASVTAVALDYGFTHLGRFAELYKSSFGILPSESLKARPPGK"
     gene            complement(20954..21820)
                     /locus_tag="PKN25_05630"
     CDS             complement(20954..21820)
                     /locus_tag="PKN25_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNV90415.1"
                     /translation="MADELVPDWGWACILQARDADWAGAGTISFTVEGVAIEIAPRGA
                     WRCARALRADTAELLDLFLPWVARPGRLAIAQLGQSLDGRIATASGASHYINCLEART
                     HLHRLRALVDAVVVGVGTVNADDPQLTVRHVAGRNPVRVVLDPRGRARADARLFQDGA
                     APTVHLVGEDLAPGTAAVLPGRVARAGLPGAGSGFEPGAVLDWLARAGCRRVLVEGGG
                     HTVSRFVQAGAIERLHLLVAPLLIGSGRAGLVLPPIDTLDEALRPVCRPFRCGSDSLF
                     DLDFDGAGRAAQ"
     gene            21918..23075
                     /gene="ribA"
                     /locus_tag="PKN25_05635"
     CDS             21918..23075
                     /gene="ribA"
                     /locus_tag="PKN25_05635"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II RibA"
                     /protein_id="HNV90416.1"
                     /translation="MRQAERALFDLRRGLSILVRDHARAFVVCAVEGLDDAAAGEMQM
                     LAGSPARLVLSDHRLAAIGRAGAAQAVSVGLSPLPDAAGLHALAWQRGASLPADAELR
                     DGGPVERAALRLLGRAQRVPAALVCEVAPDRAAAVAATVASGDLLAVDAAEAMRLCES
                     GPGRLARISEARVPLAEAENSRFVLFREADGVHEHVAIVIGERANWPEDVPVRLHSSC
                     LTGDLFGSLRCDCGEQLRRGVAAIDALGGGVLLYLSQEGRGIGLANKLRAYGLQDEGL
                     DTIDADQVIGFSKDERDFRIAHEMLDELGIDRILLLTNNPSKVAALQKAGIHVVGRRA
                     IYGEVTAQNQRYLRTKASRHGHWLNDLLNEQGEPGDDEPACLPAADAAHRA"
     gene            complement(23108..23935)
                     /locus_tag="PKN25_05640"
     CDS             complement(23108..23935)
                     /locus_tag="PKN25_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNV90417.1"
                     /translation="MNAKPALPWWQDLTPSRIAGLARADAVALLPLAAIEQHGEHLPL
                     STDLDIALGLLEAAVPKLRAGLPLCVLPPVAVGCSLEHTGFAGTLALDAKTALASVVE
                     IGACVARAGLRRLVLFNTHGGNKALVDLAALELRAAQRMLVVRAHSFRFPPPPAALPA
                     DELRHGLHGGALETALMMHLAPHKVRRDAVDRFESLGARMAAAGRLLGPEGEAGFAWM
                     AQDLHPSGACGDPRLADAALGARLVEHFASRLARIVEEAHDFDLEALVTCAPAPEQR"
     gene            24070..25794
                     /locus_tag="PKN25_05645"
     CDS             24070..25794
                     /locus_tag="PKN25_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90418.1"
                     /translation="MSARARDLVLGTLLPLLALDALLVFDNAWPTLWPRPTPAVSIEL
                     AFAVAALVLFAAWRKHAAHGLVRVLAGLATLWIVLRYVQVTVPALFGRPLNLYWDLPH
                     LGAVLDMGGDGPGAKVLLVLALGILVVLVLHRVVSACVRALARSAAAPGARALLGGVA
                     GAAILLWALAPWPGPLAASAFARPVAALVSDQIRFLHSALAADGGERLGPGPDFRGDL
                     AALRGADVLIVFAEAYGAVSFDRPPITAALADARTELGAAIAASGRQAVSARVVSPTF
                     GGASWLAHAAVLAGVDTRDPADHALLLTTDRPTLVRHFATHGYRTVGWMPGLQRPWPE
                     GRFYGFDRIADADSAGYAGLPFGFWRIPDQASMARIHVDELGGSFGEAVGTQQARSSP
                     GSSPASAGDTSASAPTSRPGARAETAAARRAPRLVVFATVSTHAPFAAIPPLREDWSR
                     LLRADAFSQEEVEAAAAVRVSWTEPLPAYLASMRYQLGWLADYLAHHAARELVLIVIG
                     DHQPIGTVSGPDQPHDVPVHVIASDPALLTRFAAAGFVAGLTPPQQPLGPMHELAQVL
                     VDAFSGPR"
BASE COUNT         3845 a         9665 c         8785 g         3448 t
ORIGIN      
        1 cgccggactg ttaatccgta ggtccctggt tcgagcccag gtcggggagc caagtttcag
       61 gacttggtgt ttcataaagg aacagcaagc cagcatcgca accgagtatt tcctcgatag
      121 ctcagtcggt agagcgccgg actgttaatc cgtaggtccc tggttcgagc ccaggtcgag
      181 gagccagcat cgccaacaag aaagccaatc cttcggggtt ggcttttttg ttggcggggc
      241 cagcgcgacg cggcactatt ccgcgccgga tcgacctcgc ctatcgcgac ttgcgtcgct
      301 cctacaggcg ggcagtcccc cgacggctca cggaagcgat gacgaccgag cccgcggtgg
      361 gagcgacgca agtcgcgata cggcgcgcga acgagcaccg gcgtccggcc ccgcgccacg
      421 atgcgtcaac gagcactgaa accgggttcg ccgccgtgcc gcgagccctc acgaattgcc
      481 cgcatcgttc cccgggtcca tctcgcgcgc ggcacgcagg tcgcggtaca ccgcggcacg
      541 catgatcagc atcgacacca ccggcgcggt catcagcacg aagacgctga tcagcagctc
      601 gtggatcacc aggcgcgact gcatcaccgt gaaatacagc atcgacgcca acaggatgca
      661 gccggcgccg agcgcgatgc tgatcgcggg gccgtggatg cgctggtaga agctctgcag
      721 gcgcgccagg ccgagcgtgc cgacgagggt gacgatgccg cccagcacca gcagcagggc
      781 caccgggatc gccgcccacc acggcaggga ttcggcaccg ctcattgggc taccctcctg
      841 tcgctgcgcg acctcgtccg cgggggagcg gagcgctttc cttcgagcgg tcgtgcggac
      901 gcgctcattc gatcacctcc ccgcgcatca ggaacttggc gagcgcgatc gtggacacga
      961 agccgagcag cgcgatgatc agcgccgcct cgaaatagat ctggctgccg tggcggatgc
     1021 ccagcagcag cgccagcacc atcacgcaca tccacagcgt gtcgagcgcc agcacgcggt
     1081 cctgtgccga ggggccgatc atcagccgca gcgtgcacaa cagcatggcc agcaccacgc
     1141 aggccagcgc gaaaagggtc gccgccgaga gcagcgtcgc ggtcgtcatg tccggcctcc
     1201 tccgggttcg ggcgtctcga acacctcgat gatcggctgt tcgtaacgcg tcttgatggt
     1261 gtgcacccac cacgcctcgt cgtgcagatc gaagacgtgg agcagcagct cgtggccgtc
     1321 gggaaggatc tccacccaca ccgtgcccgg cgtcgagttg atcaggcagg acagcagggc
     1381 caggccgtgc gggctcttca ggtcgagcgg cacgcggatg aattgcgagt tcacatcggc
     1441 cgaacgccgc aacaggatga tctgggtgac gttgaaacag gagcggacga tctcgatcgc
     1501 cgccatccac agcaggcgga acagcgccag cgggcggcgg atgcgggcgt ggccgtgcgg
     1561 ctgcagcggc tgcgacagca gcggcgcgag cacgccgagc gcgccgccga gcagccagtg
     1621 cgcgggctcc aggctctggt tgagcatcag ccacatcagc agcaggctcg ccgacagcag
     1681 cggcgaaggc agccagcgtc tcatcgcacg ccctccgcgc ccggcgcgcg cagcaccgga
     1741 ggctcgccga gcacgcgcgc cgcgtagtcg ccgctgcgca gcaggcccgc ggtggtgcgg
     1801 tcgaggtagt cgaacaccgt gcccgcctgc accgtcagca gcacgcacag gatcaccagc
     1861 gcccccaccg gcaccgcctc ggagaactgc agccgcggcg gcgtcaccgc gcccgcagcc
     1921 cagaaggtgc gcacccccag gcgcatcagc gcgatgatcg ccgccagccc ggcgaggatc
     1981 atcagcccga tcaggcccca ggtcgtcgcc gggacgcctt cgggcgcggc cgcgagcatg
     2041 gcgtggaaga ggctgaactt ggcgacgaag cccgccagcg gcggcatccc ggcgatcacc
     2101 agcgcgcacg cggcgaagct caggccgagg aaggcgagcg tgccgggaat gccgacgccg
     2161 accggatcct ccggcttgtc ctcgatcgca aaggcctcca tggtgatcgc gatcagcgcc
     2221 gcgcccggcg gacggatgcg ctcggtgagc tcgatcagca gcatgaaggc cgccatcgcc
     2281 agcgtggagc cgagcaggta gtagaggccc gaggccagca ccgccccccc ggaatgcccg
     2341 atcaccgcca gcagcgtgcc cgaggacacg atcgcgccat agcccgccat gcgcccgccg
     2401 tcctggctcg cgagcatgcc gatggcgccg aacagcacgg tcgccatgcc gccgagggtc
     2461 agcgcggcga agccgaagcc ctccgcggcg cccgccccct cgccgaacac cgccagccac
     2521 acccgcagca cggcatacac gcccaccttg gtcatcagca ccagcatcgc ccccaccggc
     2581 gccgaggccg aggcgtaggt ggtgggcaac cagaacccga gcggccacat cgcccccttg
     2641 gtcagaaagg cgagcgccag caccgccgcc ccgacctgca gcagccaggc gtcctgcgcg
     2701 ccgagcaggg ccacgcgggc gccgaggtcg gcgatgttga gcgtcccgga ggcggcgtag
     2761 atcagcgaca ccccgatcag gaacaccagc gaagccacca ggttcaccgc gatgtactgc
     2821 atgccggcct ggatgcgacg caggttgtag ccgtgcagca cgagcccgta ggacgccgcc
     2881 agcatgacct cgaagaacac gaagaggttg aacaggtcgt tggtcaggaa ggcgccgttg
     2941 agccccatca gcaggaactg gaagagcggg tggaagctca cgccgacgcg gtcccagcgc
     3001 cgcgccgaga acaccagcac ggcgagcgcg atcgtcgccg tcagcaccag catcagcgcg
     3061 gagaggcggt cggccaccag cgcgatgccg aagggcgcag cccagttggc cgccagatac
     3121 acgccgatgc cgccgggcca gtacccgccg tcggccagca ccagcagtgc gaccgccacc
     3181 gcccacagca gcatgccgct cgcgaggccg aggccgaact tgagcccgtg gcgcttctgg
     3241 ttgatcggga tcatcagcgc gccgacgatc agcggcagca gcacgggcac gatgatcagg
     3301 tggttgagcc agggcaacac cgtcattcgc gcggctcctc gccatcgacg tggtcgctgc
     3361 ccgagagccc gcgcgcgccg atcatcatca ccaggtagag cgcggtggtg gcgaagccga
     3421 tcacgatcgc ggtgagcacg aggccctgcg gcagcgggtc ggcgaactgc gccggatcga
     3481 tggcgggcgt gggcgtgatc ggcggcttgt ccacccacag ccgccccatc gcgaagatga
     3541 acaggttcac cgcgtacgac atcaacagca gcccggtgat gagctggaag gtgcgcggac
     3601 gcaggatcag ccagatcccc gcgccgaaca ccacgcctac ggaaatcgcc agtaccagtt
     3661 ccatcagatc cgcctcccct cggcaccgcc ctgtccgtcc gccgtcaggt cctcgcccgg
     3721 gtggcggtgc gcgcgcagcg actggtgcgc gagcgcgacc agcatcagca tcgtggtgcc
     3781 gaccaccacc gcgaacacgc cgaggtcgaa cacgaaggcg ctcggcaggt gcagctcgcc
     3841 caccagcggc cactccacgt gcgcggtgtg ggtggtgagg aagggatgcc cgaaccacca
     3901 cgcccccagg ccggtggcac aggccagcag cagtccgaag cccagccagc gatgcgggcg
     3961 caggttcagg cggctctcca cccacaccgt gccggcgagc atgtactgca ccaggatcgc
     4021 caccgcgaag atcagtccgg caacgaagcc gccgcccggc aggttgtgcc cgcgcatgaa
     4081 gaaatacacc gcgatcaccc ccatgaaggg cagcagcagg cgcaggtaga tccccggcac
     4141 cagcaggtag ccgctgccgg cctgctcggc ggggctctgc cggctcgccg gatcgccctc
     4201 gaagcgctgc tgcggcggga tgccggcgct ttccggcgcc gggcggaagc ggcgcagcaa
     4261 cgcatacacg gtgagcgcga cgacggcgag cacggtgatc tcgcccaggg tgtcgaagct
     4321 gcggaaatcc accagcagca cgttcaccac gttgctgccc ccgccgccgc cgagggcgtt
     4381 gagcaggaag aagtccgcga tcgtgtccga ggccggatgc accagcaccg catgggtcag
     4441 cgccgccagc gcgaggccgc cgccgacggc gagcgcgagg tcgcgcgtgc gtcgcgtcca
     4501 tacctgccac ggcggctgca ccgggccggc gatcgactcg atccgcggcg gcagccagcg
     4561 caggccgagc aggatcagca ccgtggtcac cgtctccacc atcagctggg tcagcgccag
     4621 gtcgggcgca gacagccaca ggaaggtcgc cgcggtgacg atgccggtgc cgccgacgag
     4681 cgcgagcgcg gccaggcggt ggtacttcgc catccacgcc gccgccagcg cgcagccggc
     4741 gccgatcagc cacatgccgg cgaagatggg gtcgaagccc cgcacgggga tcacggtcca
     4801 cgccggcgga gcgcccgcca gcaggagcgg gatcagcagc agcgcgagca tcatcagcat
     4861 cagctgcggc tgcagccggc gcgtgccgac gcggcgcagc agccactccg caccgcgggt
     4921 gagctgcagc atcgtcgtct cgtaggcctg cgcgccgttg aggcggtgga gcagcggcac
     4981 gcgctcgcgc cgcgccaggt cgaaatggcg gcgcagcacc ccgtaaagca gcatgccgcc
     5041 cacgagcgcg cacacgctca tcagcagcgg caggttgaag ccgtgccaca ccgccaggtc
     5101 gtaggccggc gcggcctcgc ccagggtggc gaccaccgcc aggtgcagca gcggctcgat
     5161 gctcacgccg ggggcgacgc ccaccaccag gcaggccagc accagcagct cgaccggaaa
     5221 gcgcatccag cgcggcggct cgtgggggtc gcgcggcagg ccctgcgccg gcgttccgaa
     5281 gaagaccgag atgaagcgca gcgaataggc cacgctgaac atgcccatcg ccaccgcggc
     5341 gtacgacatc caccagttgc gatccccccc gacgtgcagc gtctcggcga agaacatctc
     5401 cttggacagg aagccgttga gcagcggcac ccccgccatc gccgcgctgg cgacgatggc
     5461 cagcgtggcg gtgatcggca gcttgtggcg caggccgcgc agcacgcgca ggtcgcgggt
     5521 gccggtctcg tggtcgatga tcccggcggc catgaacagc gaggccttga aggtcgcatg
     5581 gttgaggatg tggaagatcg ccgcgacgat cgccagcggc gtgccgatgc cgatcagcac
     5641 cgtgatgagg ccgaggtggc tgatcgtcga gtaggccagc aagcccttga ggtcgtgctg
     5701 gaagatggcc acgaaggcgc cgatcagcag ggtgcacacg ccggcgccgc cgatgatcca
     5761 ggtccattcc ggcgtgccgg cgagcgccgg ccacaggcgc acgagcagga acaccccggc
     5821 cttcaccatc gtggccgagt gcaggtagga cgacaccggc gtgggcgccg ccatcgcatg
     5881 cggcagccag aagtggaagg gaaactgcgc actcttggtg agtgcaccga gcgcgaccag
     5941 caccagcgcg gggaggtaga gctcgtggtc gcggatctgg tcgccggcgg cgagcacgac
     6001 gtcgaggtcg tagctgccga cgatatgccc cagcaccaac accccggcca tcaggcacag
     6061 gcccccggtg gcggtcacgg tgaacgccat gcgcgcgccg cggcgagcgt ccgcgcggtg
     6121 gtgccagtag ccgatgagca ggaaggaggt gaggctggtg agctcccaga acaccaccag
     6181 ctgcaccagg ttgcccgaca gcacgacccc cagcatcgag cccatgaagg cgagcaggaa
     6241 ggcgaagaag cgcggcaccg ggtcttcggg cgacatgtag tagcgcgcat acagcaccac
     6301 cagcagtccg atcgccagca ccagcatgct gaagacccag gccaggccgt cgacccgcag
     6361 cacgaagtcc aagccctcgg cgggcagcca ggcgaactcc gcacgcacga cctcgccggc
     6421 ggcgacctgc gggaacaagg ccgcggcgat cgccagtccg cccaccgcca ccccgatcgc
     6481 cagccaggac tcgcggttgc gcgcgtcctg cggcatcagc gcggcgagca cgctaccgag
     6541 aaaggggagg aggacgagcg aggcgagcag catcgaggcg gatcggggcg gaaaggcgtt
     6601 cagggacggg gcgggggcca ggacgacgtg cggcggtccg gcgaaaccct gccgaggtta
     6661 ccacagcaaa tttggtgcca gattaccggg cacgcatgaa aaggcggagc tcccgcaggg
     6721 gtctgtcggg ttctgggagc gcgggagcgg cgcgctccag cgctttcgac ccgcttcacc
     6781 gacccttcca ccgccgtatc gcgacttgcg tcgctcccac aagatgcccg nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgccgtatc gcgacttgcg tcgctcccac
     6961 aagatgcccg tcgctcccac aaggtgcccg aatcacccgg tgggggcgcc ggaaccgcgg
     7021 tgttcttgtg ggagcgacgc aagtcgcgat cgccgctgaa gggtgcgatg acgctcgcac
     7081 cgccctcacc cgccgccacc ctgctccccg cgcaccagcc gcccgacatc gaagccgcgc
     7141 gccgcgaggc gttcgagcag ctcggcgtag gcacgcgcct ccaccgtcgg cgtgcgcgac
     7201 aggatccaca ggtactcccg gctcggctcg cccaccgcga ccaggcgata gtcggggtcg
     7261 aggtcgagca cccagtagtc gccccatacg atcggcagga aggacagcca ggccggcgcg
     7321 aagcgcacct ccagccgcgg cgaatcggca ccaccgatct gccgcgccac gccgaccgcc
     7381 tcggagacgc tgccatcggc ctcgcggcag cggttcacca cgcgcacctc gccgctctcc
     7441 agcaggctgt agtcggcgcg cgaatccccc acgcacttct tctggaacca gttcggatag
     7501 cgcgcgatct cgtaccagga gcccatgtag cgcggcacgt cgagcgcggc gatcggctcc
     7561 acgggcgcgg atggcgcgtc ggccgcggca acggggccga cctgcgccag gagcgccagc
     7621 acactggcgg cgagcatgag cggacggtgc ttcatcggag atcctgcatg taaatgggag
     7681 tggtacgact tcccgaggag cgtgaagttt ctcttgccgt acgcagccgc tgcgccggcg
     7741 caagcgggcc atgctggcgg ggaagcccgc aagggctcag gattgcacgc actggcgatc
     7801 gcccgccggg ccacccccag gctccgcacg cggcgcgcaa acccacgccc tgcacctgcc
     7861 cccgggagcc ccacatgaag ctgcgtctcg ccctgctgat cctcgtcctc gccgccctcc
     7921 tcgcctcggg cgtcgccccc tacgaccgcg gcgtgtggtg ggccgaggtg gccccggtcc
     7981 tgatcgcgct gcccatcctg ctcgcgagcg cgcagcgctt cccgctcacc ccgctcacct
     8041 acggtctgat cgcgttcttc tcgctgatcc tcatcctcgg cggcgcctac acctacgcgc
     8101 gcgtgccggt cggcttcgtg gtgcaggact ggttcgaact cgcgcgcaac ccctacgacc
     8161 gcttcggcca cttcttccag ggcgtcaccc cggccatcct cgggcgcgag ctgctgctgc
     8221 gcacctcgcc gctgcgcccg ggaaaatggc tgttcgccct gctcaccctg agctgcaccg
     8281 gcatcagcgc gctgtacgag ctcgtcgaat gggcctcggc cgagctgtgg gccggcggcg
     8341 cggtggaatt cctcggcacc cagggcgacc cctgggacgc ccaggccgac atgctgatgg
     8401 cgctgctcgg agcgataatc gcgcagatcg tgttcggacg gctgcacgac cgccagctcg
     8461 cacgcctcgc cgcgcagccc tccacctgaa cggacgcatc cggccgatct tcatatcggc
     8521 cgtccgtcgc ggcgactgcg gtcgccaagc cctgccgggc cgtcgcgccc ggctcatcaa
     8581 gccccggcgg cttcccgcac cggggcggct cacctatcgg gagacctcat gaaccagcca
     8641 tcctcgaacc cacgccggcg cttcctcacc gcggccggcg ggctcgccac cgccgccgtc
     8701 gccggcacgg ccctggcgca gtccgcgccc cccgccgcga gcaccgagcg cagcctgccc
     8761 ttcctgatcg gccgcgccac cgacaccctg ctgccacccg gcaagcaacg ccgcgtcgtg
     8821 atctgcggtg gcggctgggg cggggtgacc gccgccaagt acctgcgcca gctcgccccc
     8881 cagctcgacg tcgtgctgct ggagcgcaac cccgtgttct tctcctgccc gatgagcaac
     8941 aagtggctgg tcgatcaggt ggatacccgg tatctcaccc acgactacct cgccgtcgcc
     9001 gagaagtacg cctaccgctt catccagacc gagatcctcg agatcgagcg cgagggcaag
     9061 cgggtgatca cggcggccgg cgccatccac tacgactacc tgatcgtggc ccccggcatc
     9121 cgctacaact acgaggcctg gttcggcaac gaccgccgca tggccgaggc cacccgggcg
     9181 cgctttccgg cggcctacat cccgagcgcc gagcacttcc acctcaagaa cgcgctccag
     9241 tccttcaagg gcggcgacct cgtgatgacc ctgccgcccc cgccgcagcg ctgcccgccc
     9301 agcccctacg agcgcgcctg cctgatcgcg tcgatgttca agcagaagaa gatcccgggg
     9361 cgcatctaca tcctcgacca caaggacggc gcgcgcccga tcggcccggg cttccgttcc
     9421 accttcgaag agctgtacaa ggacatcatc acctacgtcc ccgacgcgcg catcgaggag
     9481 gtcgatccgt tcaagcgcag gatccgcacc gaggcgggcg atttcgactt ccagcacgcc
     9541 atcctcatgg ccccgcacca ggccggcgac ctcgcctgga aggcgggtct ggtgccgcgt
     9601 ccaggtgcgg gcaacccgag cggcggctgg gccgacgtcg atccgctctt cctgcacgac
     9661 cgcacggaca aggaggtctt cgtcatcggc gacgcggtcg gcttcgtgtc gccgcagttc
     9721 ggcttctacc ccaaggccgg ccacgtcgcc aaccgccacg cccgcatcgt ggcctactac
     9781 atctccgagc gcgaggccgg gcgggaaccc gactaccggc tcccggacaa cctctgcttc
     9841 atgatggtga atatcgcccc acaggaaggc atctcggtgc agttcgacta caaggtcaac
     9901 gcccagggca tcatcgagca gacccagatc gactacaacg agcgcaccgc ggcgacgatc
     9961 tcgcaggact tcggctgggc gcagaccatc tacgaggaca tgttctcgta agccggcccg
    10021 tccggcgcgg ggctcacacc ccgtgccggc ggcggtgctg gcgggcgccg gcggcgccgt
    10081 cggcgtccga ataggcgtgg cgcgccgcgg tcatctcggc gagcacggtg gcgatcttgt
    10141 ggttgagcgt gcccttggga atcacgccct tggcgtcggg ctcgccggcc tcgacgccgg
    10201 tcagcacggt catcgcctcg tccaccgtct tcaccgcgtg gatgtggaag cggccctccg
    10261 ccgcggcctg caccacgtcc tcgcgcagca tcaggtggcg cacgctcgcc gccgggatca
    10321 ccacgccctg cgtgccgtcc agcccgcgcg cgctgcacag gtcgaagaag ccttcgatct
    10381 tctcgttcac accgccgatc acctgcacct cgccgaactg gttcaccgag ccggtgatcg
    10441 cgaagcgctg ctggatcggc acctgggtca gcgccgagag cagtgcgcac agctcggcga
    10501 gcgaggccga gtcgccctcg accggcgcgt aggactgctc gaacaccagg ctcgccgaca
    10561 gcgacagcgg ctggtggcgg gcgtagcgcg cggcgaggaa ggccgacagg atcagcacgc
    10621 ccttggagtg gatcgcaccg ccgagttcgg tctcgcgctc gatgtcgacc acgtcgccct
    10681 cgcccatgcg caccgtggcg gtgatccgca ccgggtggcc gaagagctcc ccggcgagct
    10741 cgatcaccac caggccgttg atctggccgg cgcgctcgcc ggtggttgag atcagcgtgg
    10801 tgccgtcgag gatggactcc agtacccggg tcgggtaacg attggcccgc cgcgcgcgcg
    10861 cggcgagcgc gtcctcgacc tcggcgcgcg tcgtggcggc gaggcccttc tggcgggcgt
    10921 ggaagtcggc ctcgcgcatg aggtcggaga gcgcgcgggt gttgagcgac aggcgcccgg
    10981 agtcctcggc caggcgcgag gcttcctcga ccaggcgcgc cacaccgctg cgctcgaccg
    11041 gcagcagctt cgcgccgcgc gcgagcagcg ccatcaggcg ggcgtactcg cattcgttct
    11101 cgggcgtgcg caggatgtcg tcgttgaagt cggccgccac cttgaagagg tcggcgaact
    11161 ccggatcgtg ctccatcagc aggtagtaga cctcgcggtc gccgatcagc accaccttga
    11221 gcgcgctcgg caccgcctcc ggctccaggg tcaacgcccc gctccagccc tgcgcctcgg
    11281 ccggcggctc gatgcggatc tcgcccgcgc gcaggctgcg cttgaggcct tcccaggcga
    11341 agggctgcga cagcaggcgc tccgcgtcga gcaccaggta gccgccgttg gcgcggtgca
    11401 gcgcgccggc acggatcagg ttgaagtggc tgacctggtt gccctgctgc acgatgtgct
    11461 cgatgcggcc gatcaggttg ccgtagccgg ggttgttctc gaacaccacc ggcgcaccct
    11521 gcgagccgga atgatccacc agcagcttga cctggtagcg gtggtagcgc gtgttctcct
    11581 cgggctcggg ggcctcgtcg tcttcttcct ccagcatggt gccgcgcacc gcgctgtcga
    11641 tgacgtcgcg ctcgaccgcg tcgaggaagg cgagcacctc gggcaggtcc tcgtgcgcct
    11701 cgcgcacctc gcgcagcagg tgcgcgaccg cggggccgag cacctcgcgc tcggcacggt
    11761 cgatcgactc gcgcaccgac ttgcgccagc cggggaagtc gttgagcaga tcctcgaggc
    11821 ggtcgctcca cgcgccgatg cgcgcctcga tggccttgcg ctcgtcctcc ggcaaggcct
    11881 cgaaggcctc gggcgacagc ggctcgccgt ccttggtggg cgcgaacacg aagccctcgg
    11941 gggtctgcag cagcgagatg ccttcggcgt tgcaggcctt gccgagttcg cgcagcgccc
    12001 cttcctcgcg cgccttgtgc gcactctgca gcgactcgac acgctcgacg tgggcgtcgc
    12061 tgtcgagcgc ggcgccgagc gccgggccga ggtcgcggat gaaggtctgc atgcgcgtgc
    12121 gcagcgccgc gccgcgcccg gccggcaggg tcagcaggcg gggcttgagc ggctcgtcga
    12181 agttgtggag ataacagaga tcgggcggca cgtcctcgcg cgccgcgcgc tcgcgcagca
    12241 ggcggaaggt ggtcgcgtgc ttgccgctgc cggcatcccc gagcacgaac acgtggtagc
    12301 cggtgtggcc catcgcgagg ccgaagcgga tcgcctcgac ggcgcgctcc tggccaaggt
    12361 cgccggcgtg gtcggcgagc gattcggtgg tgtcgaagct gaagcaggcg gggtcgcagc
    12421 gcttgcgcaa agccgtcgcc ggcagtggca cgagatcggc catggatttg atctttcttc
    12481 tggtgccgga gcggcaggga gcgcaatgat acaccccgcc ccgcaggccc cgtgatccac
    12541 gcccactgcc cccggctacg gcggctccgg ttctcgtggg agcgacgcga gtcgcgatcg
    12601 gccgcgggct tgcgacgaag gtcgagatgc acgcctgcgc gaacctcgcc ttgcgccgcg
    12661 ctgtccacga ctcgataatc ccgcaacccg aaccccagcc cgcagccgca gccgaccatg
    12721 aaagtcatcc cgatccagca ggccgcgtct cccctcgccc ccgcggcccc cgcgcccgtt
    12781 gcggcgcccg gcgctgcgct ggtcgaccgc cgcgcgcgca gcgtgcgcga cctgcgcatc
    12841 tcggtcaccg accgctgcaa cttccgctgt gtgtactgca tgccgcgcga ggtcttcggc
    12901 gacgactacg ccttcctgcc gcggcggcag ctgctcagct tcgaggagat cctgcgcgtg
    12961 gcgcggctgt tcgtcgcgcg cggcgtgcgc aagatccgca tcaccggcgg cgaaccgctg
    13021 ctgcgcaagg acgtcgaccg cctgatcggc atgctcgccg cactcgacgg cgtcgaggtc
    13081 acgctcacca ccaacggtgt gctgctgccc aagctcgcgc gccggctcaa ggacgccggc
    13141 ctgcaccgcg tcaccgtgag cctggacgcg ctcgacgatg cgctcttccg gcgcatgaac
    13201 gacgccgact acccggtgga gaaggtgctc gagggcatcg aggcggccaa ggtggtgggc
    13261 ctgggcccga tcaaggtcaa catggtggtc aagcgcggca ccaacgacgt cgacatcgag
    13321 ccgatggccg cgcactttcg caacagcggc cacatcctgc gcttcatcga gttcatggac
    13381 gtgggcgctt ccaacggctg gaagatggac gaggtgctgc cctcgcgcga gatcgtcgag
    13441 cgcatcgacc gcctcttccc gctcgagccg gtcgacccca actacgaggg cgaggtcgcc
    13501 gagcgctggc gctacaagga cggcgccggc gagatcggcg tgatctcctc ggtgacccag
    13561 gccttctgct cgagctgcac gcgcatccgc ctgtccaccg agggacagct ctacacctgc
    13621 ctgttcgcgc agcagggcca cgatctgcgc gccctgctgc gcgaaggtgc cgacgatgcc
    13681 cgtatcgacg cggcgatcgc ccaggtgtgg caggggcgcg aggaccgtta ttccgagatc
    13741 cgcaccgccg agaccgccgc gctgcgcaag atcgagatgt cctacatcgg cggctgaagc
    13801 ggtcgcggcc gccgccaccg cggcctgcgc cacctcgatc gtcgcctcaa tcgtcgcgca
    13861 tcgcctccag ctcgaagcgc agcgccaccg catcgcccac catgccgcgg ctgacgccgt
    13921 agtccatgcc ccagcggctg cgctcgatgg tcgtgcgcgc cgagatgccg agcgtgtgct
    13981 tgcggtggcc gaagggatag cttgcggcct tgttgagcgt gacgtcgagc acgaccgggt
    14041 gggtctgccc gagcagggtc agacggccgg cgagcttgcc gtgcaggcgc ccgtcgcgct
    14101 cggcgccggc ctcgaagccg ctggcctcga agacgatctc gggatgcttc ctggcgtcga
    14161 ggaagtcgct gtcgcgcacg tggcggtcgc gcgcctcgtg gttgctgaac acgctgtcgg
    14221 cggccaccac caccctcccc gacttcaacg tgcgcgcggc ctcgtcgtat tcgaagcgcc
    14281 cgctcgcctt caggaacagg ccgatgatgt cggcgtagcc gaggtgctcg acctggaagc
    14341 cgatggcgaa atgctccggg tcgatcgtgt agctgcgcgg ctccgcccag gccggcgcgg
    14401 caccgaggac gcccgccagc acggccgcgg cgagcatgcg gggaaaacgt ttgcgcgatt
    14461 ccatcttcct ttctcctcca gggacttgcg aatgtggctt ccgcggtcag gattgcggac
    14521 gcgtgtggta cagccagcgc acatcgaccg cgaaggacca cgccagcagc gccagcgcca
    14581 gcaccagcag cgccgtcgcc agcgcgccgc cgatcgccgg cagcaggcat agcagcaggc
    14641 aggcgacctg ccagacgcag accagcttgc gccggcggct ctcccgcagc ggccgctcca
    14701 gccaaggcca gggccgcatc gcggcgacga agacgtagcg catcgcaccg atcgccagca
    14761 cccagggtcc cgccttgtcc atcgccagca gatgcacgca caggaccagg atcaggaagg
    14821 cgtcgagctc catgtcgaag cgcgcgccga agtcgctctc gcagcgccag cgccgcgcca
    14881 cccagccgtc cacgccatcg agcagcagcg cgagcgcggc gagcgcggcc acctgcacgg
    14941 gatgtgcatc cacccatgcg ggcgcggcaa gcatcccggc gaggagggcg accagcaggg
    15001 cgcgcaccag cgtcacccgg ttcgccggac ccaagccgcg gcgcgggtcg gaccagaagt
    15061 gccggatcaa ggcagcgatg gcgagcaaca ccgcgcccgc cgcccaagca gccgcgagcg
    15121 gcagcgcgag cgtgctcgac gccagcgcga cgagcgcgac gaggccgccg aagcccccgg
    15181 ccagatcgag ccagaggcgg ggaagtccgc cgcgccgaac acaagatccg ccctgctcca
    15241 tcgccatgct gcccttgcgc ccctttccca cctcgttcag gccccattcg cgcttggcgg
    15301 cgcgcctaca gtggctgcag cgccctgcta ccattcctcc ttcgatcatc cgccgcgcga
    15361 aagggttcgc acaaggagct cggcatgtcc agcaccaacc tcgccttctg gaccgtccgc
    15421 ccgggctacg gcgaattgcg cccggcgccg ctgcgcccgc ccgcagacgg cgagctgcgg
    15481 gtgcgcaacc tcttcggcgc agtcagccgc ggcagcgaga gcctggtgtt ccgcggcgag
    15541 gtgcccgaaa gcgaatacga acgcatgcgc gcccccttcc aggagggcga cttccccggg
    15601 ccgctcaagt acggctacat cggcgtcggc gtggtggagg acggcgtcgg caccgcggcc
    15661 gccgccttgc gcggccgcac ggtgttctgc ctgcacccgc atcagcagcg ctacgtggta
    15721 cccgccggcg ccgtcgtccc cctccccgcc ggcgtgccgg ccgcgcgcgc ggtgctggcc
    15781 gccaacctcg agaccgcgat caacgcctgc tgggacggcg cccccgcgct gggcgaccgc
    15841 atcgcggtgg tcggcgccgg cgtggtcggc agcctggtgg cctggctgtg cgcgcgcctc
    15901 cccggcgtcg agctcgagct gatcgacacc gaccccggcc gcgccggcct cgccgccgcg
    15961 ctcggcctcg ttcaccgcct cccggagcag gcgcgtggaa actgcgacct cgtcttccac
    16021 gccagcggca accccgccgg cctggtgcgc gcgctcgaac tcgccggaca ggacgccacc
    16081 gtcgtggaga tgagctggta cggccgccgc agcgcggagc tgccgctcgg cgccgccttc
    16141 catgcccgcc gcctgcgcct gcagtccagc caggtcggcc gcctgccgcc gccacgcagc
    16201 ccgcgctggg actaccgtcg ccgcatggaa ctcgcgctcg cgctgctcgt cgatccacgt
    16261 ctggacgcgc tgatcagcgg cgagaccgac ttcaccgacc tgcccgcgct gatgcagcgc
    16321 ctcgccgaag cccccgccgg ggcgctgtgc gagcgcatcc gctatgccag tccgagcacc
    16381 tgagatccac ccgagcatcg atccggagcc cccatgtaca gcctcaccgt ccgcgaacac
    16441 atcatgatcg cccacagctt ccgcggcgag atcttcggcc cggcgcaacg ggtgcatggc
    16501 gcgacctacc tggtcgacgc ctgcttccag cgcagcgagc tcgacgccga cggcctggtg
    16561 gtcgacatcg gcctcgccgc cgcaacgctg aaggcggtgc tcgccgagtt caatttccgc
    16621 aacctcgacg agctcgagga attccgcggc cgcaacacca ccaccgagtt catggccagg
    16681 gtgatcttcg accgcctcgc cgccacgatc cgcgccggcc ggctgggccc cggggcgcag
    16741 gggctggagc ggctgaaggt gagcctgcac gagtcgcacg tcgcatgggc gagctacgag
    16801 ggcgcgctgt gagcgctcac agggggagca cagacctcga tttcatcgtc cccggcgacc
    16861 ccgcccagcg caccggcggc tatctctacg atgcccgcat cgtcgaggcg ctgcgccacc
    16921 tcggctggac cgtcaccgtc catggcctgc ccggccgctt ccccgacgcc gaccccacgg
    16981 cgcgcgacgc cctgcacgac accctcgcca ccctgcccgc cggtcggcgc gtggtcatcg
    17041 acgggctcgc gctcggcggc ctgcccgagg tcgcgatcga gcacgccgga cggctggcac
    17101 tggtcgcgct ggtgcaccac cccctggccg acgagcgcgg cctcgatccc gtgctgcggc
    17161 gctgcctgct ggcgagcgaa cgggccgcgc tcgccgccgt gcgcctcgcg atcaccacca
    17221 gcgccttcac cgcgcgccgg ctgctcgact tcgggctgcg cgccgagcgc atccgctggg
    17281 tcgagccggg cgtggcgccg ctcgcgctgg ccgccgcgga gggcgagccg ccgcagctgc
    17341 tgtgcgtggc cagcctgacc ccgcgcaagg gccaggacgt gctcgtgcgc gcgctcgagc
    17401 gcgtacgagc gctgccctgg cgctgcaccc tgatcggcag cacgcatcgc gaccccggct
    17461 acgccggcga ggtggccgaa ctcgtccgca gcctcggcct gcaggaccgc atccggctct
    17521 ccggcgaatg cgcggacgcg gccctgcgcg acgcctacgc cgccgccgac ctcttcgtgc
    17581 tgccctccca ctacgagggc tacggcatgg tggtcgccga ggcgatcgcc gccgggctgc
    17641 cggtgctcgc caccaccggc ggcgcgctcg cgggcacgct gcctcccggc gccgggctgg
    17701 ccgtgccgcc cggcgacgtc gatgcgctcg ccggcgcgct tggcgagttg atcggcgacc
    17761 gcgcccggcg cctgcgcctg cgcgacggcg cccgccgcgc acgcgctgga ctgcgcggct
    17821 ggccgcaagc gggcgaggcc ttcgccgccg cccttgccga actcgcgccg gcgtccgcgt
    17881 gatcccgtcc gcgcgcccac ccggagccgc catgccgacc caccccgacg ccgtcgatgc
    17941 cccccgccat cccgacgcct gcttcgatgc cgactggctc gcgctgcgcg ccaccgccga
    18001 ccgcgccgcg cgcgcgcccc gactcgaagc gctcgccgcc gcctggctgc aggggcggcg
    18061 gcgacggcac ccgcaggccc cgctgcgcct gatggacctc ggctgcggca gcggcgccaa
    18121 cgcgcgccac ctcgcacccc gcctgcccgg cccgcagcaa tggacgctgg tcgaccacga
    18181 caccggtctt ctggaacgcg cccgcaccct cggcgcggac ctgcgcgatg cgcagggcgc
    18241 gaagccgcag gtcgacaccc gctgccggga cctcggcacg ctcgccgcac ctgcgctcgc
    18301 ggacacagac ctcgtgtgcg cctccgccgt gctcgacctc atgccagcga gctggctggt
    18361 ccgcctcgcc gatgcctgca cgagcaccgg ctgcgcgctg ctgatcacgc tgagcgtgga
    18421 cgggacctgg cgcttcctga cccacggctc cgcagagcca cccgtccccg acgatgcctt
    18481 cgtccgcgac gccttcaacg cccaccaacg ccgcgacaag ggcctcggtg ccgcgctcgg
    18541 accggacgcc gcacccgcgc tcgccgcgct gctcgcggcg cgtggcttcg acgtcgagac
    18601 cgccgccagc ccgtggcggc tggacctcgc cgccccgccc caggctgcgc tcgcatgcgc
    18661 gctcatcgac ggctggcgcg acgccgccac cgcgcagtgc ccgcacgccg ccgcgcgcat
    18721 cgccgcctgg cacgcacgcc gcagtgcgca gtgcgccgca cggggcaagg aggtgcttga
    18781 agtcgggcac ctcgacctct tcgccagccc gccggtcgcc accccggcag cgctccggcc
    18841 cgacggcccc gccgcccgcg cgcgatgagc ggcgaagccc ggcgcactcg acgcccgctg
    18901 cccgtcctgc gtgcgcttgc gagcctcggg ctgctcgccg gcgtgctgtg gtggatcgaa
    18961 ccgcgcgccc tcatcgccgc cttcggcgcg cccgcgcccg cctggctggc gctcgcgctc
    19021 gcgatcggcg tgctgcagac ggccttgtcg gcgtggcgct ggcagctcac cgccggccgc
    19081 ctcggcgccc cgctcggctt cgcccatgcg ctgcgcgagt actacctcgc gagctttctc
    19141 aaccagatcc tgccgggcgg ggtgatgggc gacgccgcgc gcgcctggcg ccacgccagt
    19201 ctgccgggca tagcccgcga cgcagcctgg caggcagtgg tgatcgagcg cggcgcgggc
    19261 cagctcgcgc tgctgctggt ggtggtcgcg agcgtgctcg ccgcccccgc gctgcaggtt
    19321 gtgccgggac gcctgggcga cgccgtcgac ctccggggac tgccgtggat gggcgtgctc
    19381 gccgtgctcg ccctcgtcgc gacggcactc gcaggccgca ccaggacggc cctgcgcagc
    19441 ctggccagcg ccacccggca ggcgctgctc gggcgcgcgg tgctgctgcg ccagctgctc
    19501 gcctcgctgc tgatcgtggc cagctacgtc gcggtgtggc tgtgctgcat gcgcatgagc
    19561 gggctcgcca cgccgccagc ccaggcggcg gcgctggtgc cctgggtgct gctggcgatg
    19621 gcgatcccgc tctcggtggc gggctggggc atccgcgaag gcgcggccgc gctggtgtgg
    19681 caggcggccg ggctggacgc cgccgaaggc gtggcggcct cggtgagcta cggcgtggtc
    19741 gtgctgctgt cgacgctgcc gggcgcgctg gcgctgcggc gccacgagcc cggcgcgcgc
    19801 tccaccgccg aggcgacgcc tacttgccgg gcggacgggc cttgaggctc tccgagggca
    19861 ggatgccgaa gctcgacttg tagagctcgg cgaagcgccc gaggtgggta aagccgtagt
    19921 cgagcgccac cgcggtgacg ctcgccacgc gcacggccgg gtccatcagg gtggcgtaca
    19981 catgctcgag cttcttgcgg cggatgtagg ttcgcgggga catccgcgcc tggtccttga
    20041 acacctgata gagcgagcgc tggctcagcc cggcgaactg ggcgagttgc tcgacgctga
    20101 tgtcctgctt gatgcgcgcc tcgatgtggc gctcgaggcg ctcgaacagg cgcgcctggc
    20161 cacaggtctg cgaggtcggc tggttatgct gcagggtgaa cagcagcttg ttggccacgg
    20221 catgggcgta gtgcatgaac agctgcgcgc gtagcgcccc gctggcgctc tcgctcgcaa
    20281 ccaggcgcag cagcgtgacc aggtggtcga gctcgtcgcg cgtgcagggg ctgcggttga
    20341 agcgcacgcc ctctcccgcg tggagccagc cgcactccga acacgcttcg cgcagcacgg
    20401 actcggggac gagcacactc cacagctcgc tatcggccga atagagcagc tcgggcgacg
    20461 caccggtgcc gagcaggatc tgtccgggct ccagctcgat cgccgcctcg ctgctgcgca
    20521 ggaaggcgtg cccgcgcacg agcacgaggg cctcgtattg ctccgcgggg ttgggcgggg
    20581 cctcgtcggg cacgacatcg gcgaccagcc ggcacaggtc caggcgctgc acactcgccg
    20641 gacgcgcgcc ggcttcccgc gcccgcggca gatcgtgcac cacaggtcgg gcggttgcgg
    20701 aggcccgcgt cgggagcggg ccttgatgcg gattggacgg caggtcgatc gacatcctca
    20761 ctccagtgaa tcggcgagcc ggctgcagcg gggtccgtcg cccactcgcc gtggagcgcc
    20821 ggtcgtcccg cagcgcagcc gaacgattca ggatcgcacg gaaacgatta cgaagacctg
    20881 cgggatcata tcagtcattc gtcgacgaga aacgcgtttg tcgatgtttt tattttatat
    20941 cgagatttca tctctactgc gccgcccgcc ccgccccatc gaagtcgagg tcgaacaagc
    21001 tgtcgctccc gcagcggaac ggcctgcaca ccggacgcag ggcctcgtcg agcgtgtcga
    21061 tcggcggcag aaccagcccg gcgcgccccg agccgatcag cagcggtgcg accagcagat
    21121 ggagccgctc gatcgccccc gcctgcacga aacgcgacac ggtgtgccca cccccctcca
    21181 ccagcacccg cctgcagccc gcccgtgcca gccaatcgag caccgcgccc ggctcgaaac
    21241 ccgacccggc acctggcagg cccgcgcgcg cgacccgccc cggcagcacg gcggcggtgc
    21301 ccggcgcgag atcctcgcca accaggtgca cggtgggcgc ggcgccgtct tggaacagcc
    21361 tcgcgtccgc ccgcgcgcga ccgcgcgggt cgagcaccac gcgcacgggg ttgcgcccgg
    21421 cgacgtggcg caccgtgagc tgaggatcgt cggcattcac cgtgccgacc cccaccacca
    21481 ccgcatccac cagcgcccgc agccggtgca ggtgggtacg tgcctcgagg cagttgatat
    21541 agtgcgaggc cccgctcgcg gtggcgatgc ggccatccag gctctgcccc agctgggcga
    21601 tcgcgagacg gccgggcctg gccacccagg gcaggaagag atcgagcagt tccgcggtgt
    21661 ccgcgcgcaa ggcccgcgcg cagcgccagg cgccgcgcgg agcgatctcg atcgccacgc
    21721 cctcgaccgt gaaacttatc gtcccggcgc cggcccaatc agcatcgcgg gcttgcagga
    21781 tgcaggccca gccccaatct ggtacaagtt cgtccgccat ccatccttcc ctgaaaaatc
    21841 gtcttcactc gcgtctgccg gcctggaaag cgcctggtgc agggtcgagc cccgacaccc
    21901 caaacggaga aaaacaaatg cgccaagccg agcgtgccct tttcgacctg cgacgcggac
    21961 tttcgatcct cgtgcgcgac cacgcgcgcg ccttcgtcgt gtgcgcggtc gaaggcctgg
    22021 acgatgccgc ggcgggcgaa atgcagatgc tggccggctc gcccgcacgc ctggtgctga
    22081 gcgaccaccg cctcgccgcg atcggccgcg ccggggccgc gcaggcggtt tccgtcggcc
    22141 tgtcgccgct tcccgacgcc gccggtctgc atgcgctcgc ctggcagcgc ggcgccagcc
    22201 tgcccgcgga cgccgaactt cgcgacggcg gcccggtcga gcgcgccgcc ctgcgcctgc
    22261 tcgggcgcgc ccagcgggtg ccggcggcgc tggtgtgcga agtcgcgccc gatcgcgccg
    22321 ctgcggttgc ggccacggtg gcgagcggcg atctgctcgc ggtcgatgcc gccgaggcca
    22381 tgcgcctgtg cgagagcggc cccggccggc tcgcccgcat cagcgaggcg cgcgtgccgc
    22441 tggccgaggc cgagaacagc cgcttcgtgc tcttccgtga agccgacggc gtgcacgagc
    22501 acgtcgccat cgtcatcgga gagcgcgcga actggcccga ggacgtcccg gtgcgcctgc
    22561 actcgtcctg cctcaccggt gacctcttcg gcagcctgcg ttgcgactgc ggcgagcagc
    22621 tgcgccgcgg cgtggcagcg atcgacgcgc tcggcggcgg cgtgctgctc tacctctcgc
    22681 aggagggtcg cggcatcggc ctggccaaca agctgcgcgc ctatggcctg caggacgagg
    22741 gactggacac gatcgacgcc gaccaggtga tcggcttctc caaggacgag cgcgacttcc
    22801 gcatcgccca cgagatgctc gacgagctcg gcatcgaccg catcctgctg ctcaccaaca
    22861 acccgagcaa ggtcgccgcg ctgcagaaag ccggcatcca cgtcgtcggc cggcgcgcga
    22921 tctacggcga ggtcaccgcg cagaaccagc gctacctcag gaccaaggcc agccgtcacg
    22981 gccactggct caacgatctg ctgaacgagc agggcgagcc gggcgacgac gagcccgcat
    23041 gcctgcccgc cgcggacgcc gcgcaccgcg cctgaggcgc ggcctccagg ccggccatgc
    23101 ggcccgctca gcgctgctcc ggcgccggtg cgcacgtcac cagcgcttcg aggtcgaagt
    23161 cgtgtgcctc ttcgacgatg cgcgcgagac ggctcgcgaa gtgttccacc aggcgcgccc
    23221 cgagcgcggc gtccgccagg cgaggatcgc cgcaggcccc cgaaggatga aggtcctgcg
    23281 ccatccaggc gaagccggcc tcgccctcgg ggccgagcag tcgccccgcc gccgccatgc
    23341 gcgcccccag cgactcgaat cgatcgaccg catcgcgcct caccttgtgc ggcgccagat
    23401 gcatcatcag cgcggtctcc agcgcgccac catgcaggcc gtggcgcagt tcgtccgccg
    23461 gcagcgcggc gggtggcggc gggaagcgga aggaatgcgc gcgcaccacc agcatgcgct
    23521 gcgcggcgcg cagctcgagc gcggccaggt ccaccagcgc cttgttgccg ccatgggtgt
    23581 tgaacagcac aaggcggcgc aagccggcgc gcgcgacgca ggcaccgatc tccaccacgc
    23641 tcgccagcgc ggtcttcgcg tcgagcgcga gcgtaccggc aaagcccgtg tgttccaggc
    23701 tgcaccccac cgcgaccggc ggcagcacgc acagcggcag gcccgcgcgc agcttcggca
    23761 cggccgcctc gagcaggccg agggcgatgt cgaggtcggt cgacagcggc aggtgctctc
    23821 cgtgctgctc gatcgcggcg agcggcagca acgcgaccgc atcggcgcgg gcgagcccgg
    23881 caatgcggct cggggtcagg tcctgccacc agggcagcgc gggcttggcg ttcatttcgg
    23941 tcggtctccc ggggggcatc ggctgacccg gatcatagcc gcactcggcc acggcccgac
    24001 gttgccgtgt cccgcgccct ggtgccgacc cgcgcgcccg cgctttgccg cacgcggcgg
    24061 aggggtcgca tgagcgcacg ggcgcgtgat cttgtgctcg gcacgctcct gccgctgctc
    24121 gcgctcgacg ccttgctggt gttcgacaac gcctggccga cgctgtggcc gcggccgacg
    24181 ccggccgtct cgatcgagct cgccttcgcc gtcgccgcac tggtgctctt cgccgcctgg
    24241 cggaaacacg cagcgcacgg cctcgtgcgc gtgctcgcgg ggctggccac gctgtggatc
    24301 gtgctgcgct acgtgcaggt gaccgtcccc gcgctgttcg gccgcccgct caacctctac
    24361 tgggacctgc cccacctcgg cgccgtgctc gacatgggcg gcgacggtcc gggcgcgaag
    24421 gtcctgcttg tgctcgcgct cggtatcctc gtcgtgctgg tactgcatcg cgtggtgtcg
    24481 gcctgcgtgc gtgccctcgc acgcagcgcg gcggcaccgg gcgcgcgtgc gctgctcggc
    24541 ggtgtcgcgg gcgcggcgat cctgctgtgg gcgctggcgc cgtggcccgg cccgctcgcc
    24601 gcgtcggcct tcgcgcggcc ggtcgccgcg ctggtctccg atcagatccg cttcctgcac
    24661 tcggcgctcg ccgccgacgg cggcgagcgg ctcggccccg gccccgactt ccgcggcgac
    24721 ctcgcggcgc tgcggggcgc agacgtgctg atcgtgttcg ccgaggccta cggcgcggtc
    24781 agcttcgatc gaccgccgat caccgcggcg ctcgccgacg cacgcaccga gctgggtgcc
    24841 gcgatcgcgg ccagcggccg ccaggccgtc tccgcccgcg tggtctcgcc gaccttcggc
    24901 ggcgcctcct ggctggcgca cgccgccgtg ctcgcggggg tcgatacccg cgacccggcc
    24961 gaccacgcgc tcttgctgac caccgatcgc cccaccctgg tccgccactt cgccacccac
    25021 ggctaccgta ccgtgggctg gatgccaggg ctgcagcgcc cctggccgga gggccgcttc
    25081 tacggcttcg accgcatcgc cgatgccgac agcgcgggct acgccggcct gcccttcggc
    25141 ttctggcgca tccccgacca ggcctcgatg gcgcgcatcc acgtggacga gctgggcggc
    25201 agcttcggcg aggccgtcgg tacgcagcag gcccgctcca gcccgggctc ctccccggct
    25261 tctgcgggcg acacctccgc cagcgcgccc acttcccgtc cgggcgcacg cgcggagacc
    25321 gctgccgcgc gtcgcgcgcc acggctggtc gttttcgcca ccgtctccac gcacgccccc
    25381 ttcgcagcga tcccaccctt gcgcgaggac tggtcgcggc tgctgcgcgc ggacgccttc
    25441 agccaggagg aggtcgaagc cgcggcggcc gtgcgggtgt cctggaccga gccgctcccc
    25501 gcttatctgg cctcgatgcg ttaccagctc ggctggctcg ccgactacct cgcccatcac
    25561 gccgcgcggg agctggtcct gatcgtgatc ggcgaccacc agccgatcgg cacggtgagc
    25621 gggccggacc agccgcacga cgtgccggtg catgtgatcg cctccgaccc cgcgctgctc
    25681 acccgcttcg cggccgccgg cttcgtcgcc ggcctgacgc cgccacagca acccctcggc
    25741 ccgatgcatg agcttgccca ggtgctggtg gatgccttct cgggcccgcg gtgagtgcgc
    25801 agcccgcatg accgcccctc cagaccgcgc aatcgcgact tgc
//
LOCUS       DAOQBS010000032        25695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1192_length_25695_cov_15.750546, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000032 DAOQBS010000000
VERSION     DAOQBS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 25695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25695
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1192_length_25695_cov_15.750546"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..1351
                     /gene="dusB"
                     /locus_tag="PKN25_05650"
     CDS             311..1351
                     /gene="dusB"
                     /locus_tag="PKN25_05650"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNV90419.1"
                     /translation="MQFAGFTLRNNLFVAPMAGVTDRPFRQLCKKLGAGVAVSEMVTS
                     NSLLYGSEKTRRRADHTGEVDPISVQIAGADPAMMAEAAKFNADNGAQIIDINMGCPA
                     KKVCNVMAGSALMQDEPLVARILEAVVKAVPDTPVTLKFRTGWNRANKNAPTIARIAE
                     ESGIRAIAIHGRTRADQYMGEAEYDTIAHVKTLVNIPVIANGDIGSPQKAKHVLDYTG
                     ADGVMIGRAAQGRPWIFREIEHFLATGELLPPPLVSEIHQVCREHLADLYAFYGDDRG
                     VKIARKHISWYTKGLVGSAAFRRAMNQIPDIPAQLAAVDDFFGQLAEAGARLSYEHER
                     APHAEYPQELAA"
     gene            1348..1584
                     /locus_tag="PKN25_05655"
     CDS             1348..1584
                     /locus_tag="PKN25_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV90420.1"
                     /translation="MSRSNEIADAVVRTLDQYFRDLDGEKPAAIHDMVIRNVERPMLA
                     FVLQQARGNQTVAAEMLGINRNTLRRKLTEYDLL"
     gene            1631..3220
                     /gene="purH"
                     /locus_tag="PKN25_05660"
     CDS             1631..3220
                     /gene="purH"
                     /locus_tag="PKN25_05660"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNV90421.1"
                     /translation="MNVTQALISVSDKRGVLDFARKLSALGIKLLSTGGTASLLREAG
                     LPVTDVSEHTGFPEMLDGRVKTLHPKVHGGILARRDLAEHMDTIAAHDIGRIDLVVVN
                     LYPFQQTVAKPDCTLEDAIENIDIGGPTMVRAAAKNHGNEQGGVGIVTDPEDYGCIIE
                     ELEANAGKLSHKTRFALAVKAFTHTARYDSAISNYLTALVTNEAGDVSLQTYPERLQL
                     AFDKVQDLRYGENPHQTAAFYRQPGAAEGGVAGYTQLQGKELSYNNIADADAAWECVK
                     AFDGSAAACVIVKHANPCGVAVAASPLEAYKKAFSTDPTSAFGGIIAFNGEVDRAAAE
                     AVSAQFLEVLIAPSYTADALELLASKKNVRVLTCALGQPAGAFDYKRVGGGLLVQSAD
                     EARIQIADLKVVTKRAPTEAEMRDMLFAWRVAKYVKSNAIVYCKDGMTIGVGAGQMSR
                     VDSARIARIKAENAGLQIAGCVVASDAFFPFRDGLDVLAQAGATAVIQPGGSMRDEEV
                     IAAANEQDIAMVFTGFRHFRH"
     gene            3255..4535
                     /gene="purD"
                     /locus_tag="PKN25_05665"
     CDS             3255..4535
                     /gene="purD"
                     /locus_tag="PKN25_05665"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNV90422.1"
                     /translation="MKVLVIGSGGREHALAWKLAQSPKVTRVLVAPGNPGTAAEKLLQ
                     NVPVTDIKVLVELAKHEHVAFTVVGPEAPLAAGVVDAFRAAGLPIFGPTKAAAQLESS
                     KDFAKQFLVRHNIPTAKYQTFADAAAAHAYVDAEGAPIVIKADGLAAGKGVVVAMTAD
                     EAHAAIDMMLLDNRMGDAGARVVIEEFMEGEEASFIVMADGKHALALATSQDHKRLHD
                     GDQGPNTGGMGAYSPAPVVTPDVHARVMREVINPTLTGMAAEGLPYTGFLYAGLMIDG
                     EGKPRVVEFNCRMGDPETQPIMMRLKTDLADLIEAAIAGKLDQTEAEWDRRFALGVVL
                     AAAGYPESPRKGDVITGLPASEQEDVHVFHAGTALQDSQVVTAGGRVLCVTALGDNVR
                     SAQKRAYEVADGIVFEGRQYRRDIGHRAIGRKTA"
     gene            4669..5577
                     /gene="hemF"
                     /locus_tag="PKN25_05670"
     CDS             4669..5577
                     /gene="hemF"
                     /locus_tag="PKN25_05670"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNV90423.1"
                     /translation="MDPRSVKPWFTELQRHIVERLEAFDGQAFRSDGWDRPGGGGGLT
                     RVIEDGNFFERGGVNFSHVMGDGMPASATAHRPELAGRRFEAMGVSLVLHPRNPYCPT
                     VHMNVRCFVALAEGKDPVWWFGGGMDLTPYYPFEEDVRHFHRSCKAAVLPWGGEAEYA
                     RLKDWCDRYFFLKHRNEPRGVGGLFFDDLGADGKTGFDTAFGLTKAVGEAFLGGYLPI
                     VERRRDIPYGERERDFQAYRRGRYVEFNLVFDRGTLFGLQSGGRTESILMSMPPVVKW
                     RYDWRPQEGSPEARLYEEFLVPRAWA"
     gene            complement(5651..6844)
                     /locus_tag="PKN25_05675"
     CDS             complement(5651..6844)
                     /locus_tag="PKN25_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HNV90424.1"
                     /translation="MRALIWLIGIFALAVGVTMLAGVNDGYVLVVLSPWRAQVSLNLF
                     IVVLVVGVALIHLLLRMVTRTVQLPGRVARWRERRRRDKADRALHGSVTALFEGRYSE
                     ALKSASTAYSASDGSPMAALVAARAAYGLQDDTRYREWLDHAAEQGKESEVARLMTEA
                     ELAIDARQFELAAARLAQLRETGQKHIAMLRLESKVAYELGRWEELVNNVRQLRKHKA
                     LTAEQAAPALRRAHVERMRQLVGDASALLGYWQEIPSEELADRGLIKEVLPLMARAGQ
                     AMHMRAQTETLLDEQWDSDLARLYASCANTMDDTNACLHKAEAWLEKQPRDAGLLFAL
                     GQLCRRVELWGKAQSYLEASLSVDPQVGTHLALAHLFETLERRDESQRHYRAAAEKMA
                     VGALA"
     gene            complement(6855..8333)
                     /locus_tag="PKN25_05680"
     CDS             complement(6855..8333)
                     /locus_tag="PKN25_05680"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNV90425.1"
                     /translation="MRQENPALTQPPPATSASPAPEETRPVIEEVGVPEAPPAAEVDP
                     AARGAASEGDPVAPAARTANAAEEQPARSPAAWWALLLALIAVGVAGWSIWQAREMRL
                     QTGQLRAEVASRLSDGETIATEARGMMRQQQEVIASLQGKLGALESRVETTQGQAEAL
                     EALYQEFSRSREDGVLAEVEQAVALASQQLQIAGNVEAALIGLQEAEARLAIHDRGQL
                     ATLRRALVRDIEELRLLPVLDVSGLSLRLELMLERADTLPLAFESPLPAAAAVGAEMG
                     PADGGGFVGWMAGVWRFAQNVAADAWSEIRTLIRVERLDQEDPVLLAPEQNTFLRENL
                     KMRLLTARLALMARDGRSYAADLAQARQWLERFYDLRDERVQAALGELKQLEAVKVRY
                     APPDLSETFSALRSVQSRAGRSGADARGAAAPAQAAPAAAAAPAPASAPAEAPVATAE
                     TPASAAQGAANQPAAAEAQPAAPAEQAPAGSVTDAAAPAASQ"
     gene            complement(8344..9078)
                     /locus_tag="PKN25_05685"
     CDS             complement(8344..9078)
                     /locus_tag="PKN25_05685"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNV90426.1"
                     /translation="MRPREQAESLCARLEAAGGRALRFPVIAVGPALDPAPLQAIVGR
                     LDEFDLAFFVSPNAVDHAMRAILPQRTWPARLRVATVGKGSQRVMQDWGFANVIAPQD
                     GFDSEAVIALPEFAADAVRGRKVLIFRGDGGRELLADTLRERGASVEYVTCYRRHCPE
                     ADPALLLGPAARGELDGLLLTSSEGVRNLECMLGTEGLAALRAVTVFATHPRIVAQAR
                     AAGFERVVETPAGDDGLMQALESHFA"
     gene            complement(9171..10103)
                     /gene="hemC"
                     /locus_tag="PKN25_05690"
     CDS             complement(9171..10103)
                     /gene="hemC"
                     /locus_tag="PKN25_05690"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNV90427.1"
                     /translation="MSQSSPERIVIATRESRLALWQAEHIKARLEALHPGCRVELLGM
                     TTRGDQILDRPLAKVGGKGLFVKELETALLDGRADIAVHSMKDVPMVMEPEFALPCIT
                     AREVPLDAFVSSRYESLADMPPGAVVGTSSLRRESQIHAQYPFLGVTSLRGNLDTRLR
                     KLDEGQYDAIILAAAGLTRLGLGERIRSVMPAEVSLPAAGQGALGIECLSARADVIGW
                     LQPLVDADTTACVLAERAVARALAGSCEVPLGAYAVMREGRLWLRGFVARPDGSTIVR
                     AEREGAPAEAEALGRALADDLRAQGAEAILAELG"
     gene            10361..13123
                     /gene="ppc"
                     /locus_tag="PKN25_05695"
     CDS             10361..13123
                     /gene="ppc"
                     /locus_tag="PKN25_05695"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNV90428.1"
                     /translation="MNQDKDAPLRDDIRLLGRLLGDTVRDQQGEAAFGLIERIRQNSV
                     RFRRDDDIAARRELEDILDALSREQTIQVVRAFSYFSHLANIAEDQHHIRRSRAHLIA
                     GSAPREGSLAHALERALDSGAADPAALAGFFDGALVSPVLTAHPTEVQRKSILNCETV
                     IARLLDARDRMQLTPEEAEANDEALRRAVLTLWQTRMLRTAKLSVIDEVNNGLSYFET
                     TFLRELPRLYASLEDRLAAADRALGTPELAAFVQVGSWIGGDRDGNPFVTAEVLERAL
                     AMQCAVALGHYLDELHILGSQLSLGIGLVSASDALLALAEASPDHSPHRSDEPYRRAI
                     SGIYARLAATYRSLLGHDPLRHAVAAAQPYPSAAALAEDLEVLHRSLAANGSGALTRG
                     RLRHLRRAVKVFGFHLAPLDLRQNSDVHERTVAELLERACPGTAYASLDEDARCALLL
                     EELATARPLASPHVRYTDETEGELAIFRAARQAHLRYGTAAIRNCIISKTDDVSDLLE
                     LAVLLKEAGLLRPLENALDVNIVPLFETIGDLENAAGVMARIFSIPAYRGLLEARDHT
                     QEVMLGYSDSNKDGGFLTSGWALYKAEGELVETFARHGVRLRLFHGRGGSVGRGGGPS
                     YQAILAQPEGAVQGQIRLTEQGEVIGAKYGNPEVGRRNLEVLVAATLESSLRPASASP
                     TPPAFLDAMQALSDAAFAAYRGLVYETEGFERYFWESTVISEIAALNIGSRPASRKKS
                     TAIEDLRAIPWVFSWSQCRVMLPGWYGFGSAVRDFLTANPDDGLALLQRMNMEWPFFQ
                     TLLSNMDMVLSKTDLAIASRYAELVKDAALRDTIFGRIRAEHQATVGAVLRITGQSEL
                     LDANPLLKRSIRNRFPYLDPLNHVQVELLRRHRDAAEAGGIDERIRLGIHISINGIAA
                     GLRNSG"
     gene            complement(13130..13924)
                     /locus_tag="PKN25_05700"
     CDS             complement(13130..13924)
                     /locus_tag="PKN25_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNV90429.1"
                     /translation="MTATAPEAVAALRVLVVDDEAPARARLRDLLGDVAGQQPNAIVG
                     MAANGVEALRLLDAAAADVVLADIRMPVMDGVELARHLARLPAPPALVFVTAYDQYAV
                     QAFELAAVDYLLKPVRAQRLAAALERVRARRAPPAADVLATLATRARRHFSVAECGRI
                     LLVPVAEVRYLRAELKYVTARTAAREYVLDESLVQIEQEFPRSFLRLHRNCLVALAAV
                     AGVERAAERDGQKEAEPRWEVLLRDLPERLPVSRRQWPAVRQALGL"
     gene            complement(13921..15030)
                     /locus_tag="PKN25_05705"
     CDS             complement(13921..15030)
                     /locus_tag="PKN25_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNV90430.1"
                     /translation="MAESIKQKPPALGAPGLPDFRNLGVMLRVLLAVNLFAALTVAIR
                     LDAPARLADELALMAGRVELPLLLSLVLLYLGAPGLSALPVAAGRGVVLVLASLAAIA
                     ASLLAAVPAAEWWRPLGWAWLAAALCLAYFDWRSRRLSPALAEARLIALTARIRPHFF
                     FNCLNGVLGVIRSEPRRAEQALEELADLFRALMQDGRERVTLAEEIELAERYIGLERL
                     RLGERLAVRWEFGGGGGEAGEDGEGGEGGEGGRGGEGGERRTRVPAELLRARMPSLLL
                     QPLLENAVYHGIEPAAAAGEVIVRIARHGKELCIEVDNPVAAQGLHHVGNRMALDNIR
                     ERLMLFHDLEARLEIDEADGRYRVRIRLPWEREAA"
     gene            15127..16584
                     /gene="argH"
                     /locus_tag="PKN25_05710"
     CDS             15127..16584
                     /gene="argH"
                     /locus_tag="PKN25_05710"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNV90431.1"
                     /translation="MTDTTTPAASANAPADASAKAWSGRFSEPVSELVKRYTASVSFD
                     QRMAAQDIRGSLAHARMLARQGIIGTQDLVDIERGMAQIAGEIERGEFAWSLDDEDVH
                     LNIEKRLTALVGDAGKRLHTGRSRNDQVATDIRLWLRDAIDRILGLIAEFQSRLLEVA
                     ETHAGTPLPGFTHLQVAQPVTFGHHLMAYFEMSRRDAERFADCRKRVNRLPLGSAALA
                     GTSYPIDREFVASELGFDEVCYNSLDAVSDRDFAIEFCAASALLMTHLSRLSEELILW
                     MSPRVGFIDLADRFCTGSSIMPQKKNPDVPELVRGKTGRVNGSLVALLTLMKGQPLAY
                     NKDNQEDKEPLFDTADTVIDTLRIYADMIGGIRVKAEAMRGALSQGYATATDLADYLV
                     KKGLPFRDAHEAVALAVRAAEVKGCDLPQFTLDELRIAMAHVPGAAERLGEDVFGVLT
                     VEGSLASRKHIGGTAPEQVRAAVARARIGLGLGQG"
     gene            16601..17896
                     /locus_tag="PKN25_05715"
     CDS             16601..17896
                     /locus_tag="PKN25_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNV90432.1"
                     /translation="MTTAAAERIGKYELLRRIGKGATSSVHLAWDPFRQVEVAIKRLR
                     REILADPQRGRLLRQLFLNEAALAGKLAHPHIVRIHDALVTDEDAWLVMDHVPGGTLE
                     DIATPATLAPFGRVIEIVFKATRALDYAFHQGITHRDIKPANILLMAPGGSDIRISDF
                     GAALHTARETTQIAGVGSPAYMSPQQVREVPLDHRTDIYSLGVVMFQLLTGHLPFEQE
                     NSYSLLYSIAHDTPPPPSELRPEVPEALDAIVRRATEKDIERRYQSWSEFSHDLALAF
                     RNRSLALAPDRLGDTGKFEALRTMRFFRRFADAELWEVIGLSRFDRALAGTVIIKEGE
                     RGDAICFVLAGEARVSKQGRLLEVLNPGDCFGEIAVFSTISGVRSASVEAAADTELVT
                     IRARALERASETLRMHFYQAFLEVLATRLSQASARIANL"
     gene            17893..18816
                     /locus_tag="PKN25_05720"
     CDS             17893..18816
                     /locus_tag="PKN25_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HNV90433.1"
                     /translation="MNESPTPHPALPAVEAALRAAMGEQLRIVSAEPVSGGSIHTALR
                     LQDAGGARFFAKLAPAAQAPMFEAEADGLGAIAASGSLRTPAVIARGGDEAHAILVLE
                     WLDLQPLTSAADGARFAEALAALHRNVGEHFGWPRDNFIGRTPQANTERDNWARFFVE
                     QRLRPQFARARAQGFDIELQRQADRLLDRVPALFLDYRPPESLLHGDLWHGNAAVLAD
                     GTPVVFDPAVHRGDREADLAMSELFGGFPSAFYTAYRRAWPLHEDYEQRKPLYSLYHL
                     LNHLNLFGRAYLRESLRLATRLNEELARRRD"
     gene            18838..19590
                     /locus_tag="PKN25_05725"
     CDS             18838..19590
                     /locus_tag="PKN25_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90434.1"
                     /translation="MRRRFLHSAPERDLPAQHTYFEISDLRRFFDAGVIFATVWVRFI
                     GKTSGGRYNEIRHQRNQGLMMDRIRNNETVEQRRRVLKGALGASTVLTLGYGGSAAAA
                     SIGCVKNTYIEETPPANGNQFSWTDPAAMTGVDEWAWSKVDVWECSGNVGGVPDTDFE
                     GFSLDNGINWYRVPAVVGEAPTLIALATKKSSQSPAYPKTAWVLAYFDANGNFVGNYP
                     AITMRTEQNTPLEGSCLASVNPNAKSGFTYGG"
     gene            19597..19884
                     /locus_tag="PKN25_05730"
     CDS             19597..19884
                     /locus_tag="PKN25_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90435.1"
                     /translation="MQPTSPLGPLAWIERYCPSLDGQFLFLDPLRWDTHLLSAGAVIV
                     LREAALAIEAGCFEAFRAEVAANGGWPAGLERLAVALTALAERAAGTGTEA"
     gene            19896..20753
                     /locus_tag="PKN25_05735"
     CDS             19896..20753
                     /locus_tag="PKN25_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HNV90436.1"
                     /translation="MQRSIDLGAASVVVRSPLREFNAQLDLLYRDHSQAGDSGFADVR
                     VALLPGPPWRVWGPQVRLWSEASCPFDPYPLDSALPLFEWGTNWLLAQRLNAHVLLHA
                     GVVAREDRALILPAAPGSGKSTLTCALHLAGWRFLSDEFGVLDTESGLLHPLLKPAAL
                     KNRSIDIIGGQPGAVLGPVFAGTRKGDVAHFAPDRASVAARRRPVTPALVVFPRYREG
                     AALRCEPVRRAEAAMRLGLNSFNYRKLGPVGFDAILRLARTAPAWEIEYGALADALPC
                     IEQLFGKAS"
     gene            20750..21853
                     /locus_tag="PKN25_05740"
     CDS             20750..21853
                     /locus_tag="PKN25_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNV90437.1"
                     /translation="MSTHPAADCALLLRGLHEALEIPEARWDRVLRLARMTGLHGRLA
                     AANADNPTVSERVRRHLLSATRMADFSARMLDAELRDVAPLCGGDFPVIALKGGAYAL
                     QALGFARGRFVSDIDLLVPARALRTMEARLHAAGWESAALDPYDERYYRDWSHETPPL
                     RFPGHFLELDLHHAITPVTGRRHFDPAPLFDESLPIAGSRFRVLSARDQVLHACLHCF
                     QDGDLALRVREIADIDGLLRSFAGTTGFWPGLVERANALGLGRPLWYGLHFARSWMQT
                     PVPEAVLSALPGPAAVLRRPMEACISLALLPPDPDRPPGLSVRFARRLALARYHLLRM
                     PLHMLLPHLARKAWLRTRRRTPDGANENAGGGP"
     gene            complement(21901..22230)
                     /gene="grxD"
                     /locus_tag="PKN25_05745"
     CDS             complement(21901..22230)
                     /gene="grxD"
                     /locus_tag="PKN25_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNV90438.1"
                     /translation="MDIQDVIRDQVTNNGVVLYMKGTPQFPQCGFSSTAVQILKGCGV
                     REVLAVNVLADPEIRQGIKEFSNWPTIPQLYVKGEFVGGSDIMREMYDSGELQQMLKD
                     AGLVGED"
     gene            complement(22295..23164)
                     /gene="prmC"
                     /locus_tag="PKN25_05750"
     CDS             complement(22295..23164)
                     /gene="prmC"
                     /locus_tag="PKN25_05750"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNV90439.1"
                     /translation="MSPAAPSADGIDIAGALAWARARIDQMDARVLLRHVLQCPAARL
                     VAWPEQKLAAEDWAEYRALVERRAAGEPVAYLTGTREFYGREFLVTPAVLIPRPETEL
                     LVELALAHFPGRRGLRVLDLGTGSGALAVTLALELEAAEVVALDRSREALWVAMANAA
                     RLGASVSFVQSDWFGALGDEHFELIVSNPPYVAAGDPHLEQGDVRFEPRGALAAGPQG
                     LDDLAEIVAGAPARLVDGGWLFLEHGYDQAASARGLLADAGFAAIASWADLAGIERVS
                     GGRWLGRATRDSR"
     gene            complement(23161..24243)
                     /gene="prfA"
                     /locus_tag="PKN25_05755"
     CDS             complement(23161..24243)
                     /gene="prfA"
                     /locus_tag="PKN25_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNV90440.1"
                     /translation="MKQSIRDKLEHLANRLEELDRTLASEEGARDMNAFRELSRERAE
                     IEPVVALYREHRQAEADCATARELLADPEMRELGELELAEGEARIEALEAELQRALLP
                     RDPNDARNLFLEIRAGTGGDEAALFAGDLLRMYTRYAERQRWKVEIVSASESDLGGYK
                     EVIARVVGNGAYSKLKFESGGHRVQRVPVTETQGRIHTSACTVAVLPEADEVEAVDIN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHIPTGIVVECQDDRSQHRNKAQAMAVLAA
                     RLYDARVRAQQSAEAAQRKSLVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLDAVMQ
                     GELDEIFDALTLEHQADLLAALGEDA"
     gene            complement(24301..25557)
                     /gene="hemA"
                     /locus_tag="PKN25_05760"
     CDS             complement(24301..25557)
                     /gene="hemA"
                     /locus_tag="PKN25_05760"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNV90441.1"
                     /translation="MQLFALGLNHHTAPLVIRERVAFQPERMGQALHDLAHTDSVREA
                     AILSTCNRTELYFAAEQPQHAADWLARFHRLALAEVSPYLYSYPQRDAIRHVFRVASG
                     LDSMVIGEPQILGQVKDAARYAEEAGTLGVLLHKLFQNTFAVAKEVRSTTAIGANTVS
                     MAAAAVHLTERIFERVADQHVLFIGAGEMIELCAAHFGGARPKSMAVANRTEERARAL
                     ADRFGATTMRIDQIGEALPRFDVVVSCTAAPLPIVGLGMVERAVKARRHRPMVMVDLA
                     VPRDIEPEIAELDDIFLYTVDDLAQVVEAGMESRQQAVIEAEQIIASRVDGFLHWMSA
                     RETVPVIRSLREHAESLRQAELERAVRLLAKGEDPQRVLEALSHGLTNKLMHGPTRFL
                     NQAEGEHKVEAGRVVRELFNLSRDSH"
BASE COUNT         3940 a         9463 c         8641 g         3651 t
ORIGIN      
        1 gtcgccgccc ccgcgacgtc ctccgccgag ggcagcccgc agcgcagcga agggccgcga
       61 gggcaggcgg cgacccccac cccggccccg cgccgcgcac tgcagcagtt gccctttcgc
      121 actcaccgcc ccctgcctcc gcgcacacca cgacacgcag agcgcgccac gcccgacgca
      181 ccatccgtcg tgcgcccgac aggcgcctcc agggagctgc gcagcatcag cgtgttgcac
      241 acgcgcccca acaagcgcac ggccaggatc gatagggtaa aatgcttcgt ttcccctcgt
      301 ccttccaacc atgcagtttg ccggcttcac cctgcgcaac aacctcttcg tcgcccccat
      361 ggccggcgtg acggaccgcc ccttccgcca gctctgcaaa aagctgggcg caggcgtcgc
      421 cgtatccgag atggtcacct ccaattcgct gctctacggc agcgagaaga cccgccgccg
      481 cgccgaccat accggcgagg tcgatccgat ctcggtgcag atcgccggcg ccgatccggc
      541 gatgatggcc gaagccgcga agttcaacgc cgacaacggc gcgcagatca tcgacatcaa
      601 catgggctgc ccggccaaga aggtgtgcaa cgtgatggcc ggctcggcgc tgatgcagga
      661 cgagccgctg gtcgcgcgca tcctcgaggc cgtagtcaag gcggtgcccg ataccccagt
      721 cacgctcaag ttccgcaccg gctggaaccg cgccaacaag aacgcgccga cgatcgcgcg
      781 catcgccgag gagtccggca tccgcgccat cgccatccac ggccgtaccc gggccgacca
      841 gtacatgggc gaggcggagt acgacaccat cgcccacgtc aagaccctcg tgaacatccc
      901 ggtgatcgcc aacggcgaca tcggcagccc gcagaaagcc aagcacgtgc tcgactacac
      961 cggcgccgac ggcgtcatga tcgggcgcgc ggcgcagggc cggccgtgga tcttccgcga
     1021 gatcgagcat ttcctcgcca ccggcgagct cctgccgccg ccgctggtga gcgagatcca
     1081 ccaggtgtgc cgcgagcacc tcgccgacct ctacgccttc tacggcgacg accgcggcgt
     1141 caagatcgcg cgcaagcaca tctcctggta caccaagggc ctggtgggct cggcggcctt
     1201 ccgccgcgcg atgaaccaga tccccgacat tcccgcccag ctcgccgcgg tggacgactt
     1261 cttcggccag ctcgccgagg ccggcgcccg cctgagctac gagcacgagc gcgcgccgca
     1321 cgccgaatat ccgcaggaac tcgcagcatg agccgcagca acgagatcgc ggacgccgtc
     1381 gtccgcaccc tcgaccagta tttccgagac ctcgacggcg agaagccggc ggcgatccac
     1441 gacatggtga tccgcaacgt cgagcgtccg atgctggcct tcgtcctcca gcaggccagg
     1501 ggcaaccaga ccgtggccgc cgagatgctc ggcatcaacc gcaacacgct gcggcgcaag
     1561 ctgaccgaat acgacctgct ctgagcgggt gcttcccctt ttcctccatc cttccgcttc
     1621 cggcccaacg atgaacgtga cccaagccct gatcagcgtc tccgacaaac gtggcgtgct
     1681 cgacttcgcc cgcaagctct ccgcgctcgg catcaagctg ctgtcgaccg gcggcaccgc
     1741 cagcctgctg cgcgaggccg gcctgccggt gaccgacgtc tccgagcaca ccggcttccc
     1801 cgagatgctg gacggccggg tcaagaccct gcacccgaag gtgcatggcg gcatcctcgc
     1861 ccgccgcgat ctcgccgaac acatggacac catcgccgcc cacgacatcg gccgcatcga
     1921 cctggtggtg gtcaatctct accccttcca gcagaccgtg gccaagcccg actgcacgct
     1981 ggaagacgcg atcgagaaca tcgacatcgg cggccccacc atggtgcgcg ccgcggccaa
     2041 gaaccacggc aacgagcagg gcggcgtcgg catcgtcacc gaccccgagg actacggctg
     2101 catcatcgaa gagctcgagg ccaacgccgg caagctcagc cacaagaccc gcttcgcgct
     2161 cgcggtgaag gccttcaccc acaccgcgcg ctacgactcg gcgatctcca actacctcac
     2221 cgcgctcgtc accaacgagg ccggcgacgt gtcgctgcag acctaccccg agcgcctgca
     2281 gctcgccttc gacaaggtgc aggacctgcg ctacggcgag aacccgcacc agaccgcggc
     2341 cttctaccgc cagcccggcg cggccgaggg cggcgtggcc ggctacaccc agctgcaggg
     2401 caaggagctg tcctacaaca acatcgccga cgccgacgcg gcctgggaat gcgtgaaggc
     2461 cttcgacggc tcggcggcgg cctgcgtcat cgtcaagcac gccaatccct gtggcgtggc
     2521 cgtcgccgcc agcccgctcg aggcctacaa gaaggccttc tccaccgacc ccacctcggc
     2581 cttcggcggc atcatcgcgt tcaacggcga ggtcgaccgt gccgcggccg aggccgtttc
     2641 ggcacagttc ctcgaggtgc tgatcgcgcc gtcctacacc gccgacgcgc tcgagctgct
     2701 cgcgagcaag aagaacgtgc gcgtgctcac ctgcgcgctc ggacagcctg ccggtgcctt
     2761 cgactacaag cgcgtcggtg gcggcctgct ggtgcagagc gccgacgagg cccgcatcca
     2821 gatcgccgac ctcaaggtcg tcacgaagcg ggcgccgacg gaagccgaga tgcgcgacat
     2881 gctcttcgcc tggcgcgtgg ccaagtacgt caagtccaac gccatcgtgt actgcaagga
     2941 cggcatgacc atcggcgtcg gtgccggcca gatgagccgc gtcgactcgg cgcgcatcgc
     3001 caggatcaag gccgagaacg ccggtctgca gatcgccggc tgcgtggtcg cctcggacgc
     3061 cttcttcccc ttccgcgacg gcctcgacgt gctcgcccag gcgggtgcga ccgcggtgat
     3121 ccagcccggc ggatcgatgc gcgacgaaga ggtgatcgcg gcagccaacg agcaggacat
     3181 cgccatggtg ttcaccggct tccgtcactt ccgtcactaa gaacgaccac cccagcagag
     3241 cttcggagca gcacatgaaa gtcctcgtca tcggttccgg cggccgcgaa cacgcgctgg
     3301 cctggaaact tgcccagtcg cccaaggtca cccgcgtgct ggtggcgccg ggcaaccccg
     3361 gcaccgctgc cgaaaagctg ctgcagaacg ttcccgtcac cgacatcaag gtgctggtcg
     3421 agctcgccaa gcacgagcac gtcgccttca ccgtggtcgg ccccgaggcg ccgctggcgg
     3481 ctggcgtggt cgatgccttc cgcgccgccg gcctgccgat cttcggtccg accaaggccg
     3541 ccgcccagct cgagagctcg aaggacttcg ccaagcagtt cctcgtccgc cacaacatcc
     3601 cgaccgcgaa gtaccagacc ttcgcggatg ccgccgccgc acacgcctac gtcgacgccg
     3661 agggtgcgcc gatcgtgatc aaggccgatg gcctggccgc gggcaagggc gtggtggtgg
     3721 cgatgacggc cgacgaggcg cacgccgcga tcgacatgat gctgctcgac aaccgcatgg
     3781 gcgatgcggg tgcgcgcgtc gtcatcgagg agttcatgga gggcgaggag gccagcttca
     3841 tcgtcatggc cgacggcaag cacgcgctcg cgctcgccac cagccaggac cacaagcgcc
     3901 tgcacgacgg cgaccagggc cccaacaccg gcggcatggg cgcctactcg cccgccccgg
     3961 tggtcacccc cgacgtgcat gcgcgcgtga tgcgcgaggt catcaacccg acgctgaccg
     4021 gcatggccgc cgagggcctg ccctacaccg gattccttta cgccgggctg atgatcgacg
     4081 gcgagggcaa gccgcgcgtg gtcgagttca actgtcgcat gggcgatccc gagacgcagc
     4141 cgatcatgat gcgtctgaag accgatctcg ccgatctgat cgaagccgcc atcgccggca
     4201 agctcgacca gaccgaggcc gagtgggacc gccgcttcgc gctcggcgtc gtgctcgccg
     4261 ctgcaggcta cccggagagc ccgcgcaagg gcgacgtcat caccgggctg cccgcctccg
     4321 agcaggagga cgtgcatgtc ttccacgccg gcaccgcgct gcaggacagc caggtcgtca
     4381 ctgccggcgg gcgcgtgctg tgcgtgaccg cgctcggcga caacgtgcgc agcgcgcaga
     4441 agcgcgccta cgaggtcgcc gacggcatcg tcttcgaagg ccgccagtac cgccgcgaca
     4501 tcggccaccg cgcgatcggc cgcaagaccg cctgatcgcc gggcgccgtg caggcgcccc
     4561 atcgccgtct ccgcctccgc gagcgacggc gtagctctca cccagcgccg acgtccgcgc
     4621 gcgaagctct cgcggggcgc tggcgcgcca atgccacgga ggtcccgcat ggatccacgc
     4681 agcgtcaaac cctggttcac cgagctgcag cgccacatcg tcgagcgcct cgaggccttc
     4741 gacggccagg ccttccgcag cgacggctgg gaccgcccgg gcggtggcgg cggcctcacc
     4801 cgagtgatcg aggacggcaa tttcttcgag cgcggcgggg tgaacttctc gcacgtgatg
     4861 ggagacggca tgcccgcctc ggccacggcg catcgccccg agctcgccgg ccgccgcttc
     4921 gaggccatgg gcgtgtcgct ggtgctgcat ccgcgcaatc cgtattgccc caccgtgcat
     4981 atgaacgtgc gctgcttcgt cgcgctggcc gaaggcaagg atccggtgtg gtggttcggc
     5041 ggcggcatgg acctgacgcc ctactacccc ttcgaggagg acgtgcgcca cttccatcgc
     5101 agctgcaagg cggcggtgct accctggggc ggcgaggccg agtacgcccg cctcaaggac
     5161 tggtgcgacc gctacttctt cctcaagcac cgcaacgagc cgcgcggcgt gggcgggctg
     5221 ttcttcgacg acctcggcgc cgacggaaag accggtttcg acaccgcctt cggcctcacc
     5281 aaggcggtgg gcgaggcctt cctgggtggg tacctgccca tcgtcgagcg ccgccgcgac
     5341 atcccctacg gcgagcgcga acgcgacttc caggcttacc ggcgcggccg ctacgtcgag
     5401 ttcaatctcg tcttcgaccg cggcacgctg ttcggcctgc agtcgggcgg acgcaccgaa
     5461 tccatcctga tgtcgatgcc gccggtggtg aagtggcgct acgactggcg cccgcaggag
     5521 ggctcgcccg aggccaggct gtacgaggaa ttcctcgtcc cgcgcgcttg ggcctgattt
     5581 gtcggatcgc gagcagcgga caaaaaaacg gggcctcgcg gccccgggat ctctctgcgt
     5641 gattcttcgc tcaggccagc gcgccgaccg ccatcttctc ggccgcggcg cggtagtggc
     5701 gctgggactc gtcacggcgc tccagggtct cgaagagatg cgcgagcgcc agatgggtgc
     5761 cgacctgcgg gtcgacgctc aacgaggcct cgagatagct ctgcgccttg ccccatagct
     5821 ccacccgacg gcaaagctgg ccgagggcga acagcaggcc cgcgtcgcgc ggctgcttct
     5881 ccagccaggc ttcggccttg tgcaggcaag cgttggtgtc gtccatggtg ttcgcacagc
     5941 tcgcgtacag gcgcgccaga tcgctgtccc actgctcgtc gagcaaggtc tcggtctgcg
     6001 cgcgcatgtg catcgcctga cccgcgcgcg ccatcagcgg cagcacctcc ttgatcagcc
     6061 cccggtcggc aagctcctcg gagggaatct cctgccagta cccgagcaag gcggacgcat
     6121 cacccacgag ctggcgcatg cgctcgacgt gggcacgacg cagcgcgggc gcagcctgct
     6181 ccgcggtcaa cgccttgtgc ttgcgcagct ggcgcacatt gttcaccagc tcttcccagc
     6241 gcccgagctc gtaggccacc ttgctctcca ggcgcagcat cgcgatgtgc ttttgccccg
     6301 tctcgcgcag ttgcgccagg cgtgccgccg cgagctcgaa ctggcgggca tcgatggcga
     6361 gctcggcctc ggtcatcagg cgggccacct cgctctcctt gccctgttcg gcggcgtggt
     6421 ccagccactc gcggtagcgg gtgtcgtcct gcaggccgta ggcggcgcgc gctgcgacca
     6481 gcgcggccat cggcgaacca tcgctcgccg aataggcggt cgaggcggat ttgagggctt
     6541 ccgagtaacg cccctcgaag agcgccgtca ccgagccgtg cagcgcccgg tcggccttgt
     6601 cgcgacgccg gcgctcgcgc cagcgcgcca cccggccggg cagctgcacg gtacgggtca
     6661 ccatgcgcag cagcaggtgg atgagggcga cgcccaccac cagcaccacg atgaagaggt
     6721 tcagcgaaac ctgggcccgc cacggcgaca gcacgacgag aacgtagccg tcattcacgc
     6781 cggccagcat cgtcacgccg acggcgaggg cgaagatgcc gatcagccag atcagtgcgc
     6841 gcatcgttcg gccctcactg gctcgccgcc ggggcggcag cgtcggtcac ggagcccgca
     6901 ggcgcctgct cggccggcgc agcgggctgc gcctcggcag cggcaggctg gttcgccgct
     6961 ccctgggctg cggatgccgg ggtctcggca gtcgccacgg gcgcttcggc aggggcggac
     7021 gcaggagcgg gcgcggcagc ggctgcaggc gctgcctgcg caggcgcggc agcgccgcgc
     7081 gcatccgcgc cgctgcggcc agcgcgcgac tgcacgctgc gcaaggcgct gaaggtctcg
     7141 ctcagatcgg gcggcgcgta gcgcaccttc accgcctcga gttgcttcaa ctcgccgagc
     7201 gcggcctgca cccgctcgtc gcgcaggtcg tagaaacgct ccaaccactg gcgggcctgg
     7261 gcaagatcgg ctgcgtagct gcggccgtcg cgcgccatca gcgcgaggcg cgcggtgagc
     7321 aggcgcatct tgaggttctc gcgcaggaag gtgttctgct cgggcgcgag cagcaccgga
     7381 tcttcctggt cgaggcgctc gacgcggatc agggtgcgga tctcgctcca ggcatcggcc
     7441 gcgacgttct gcgcgaagcg ccacacaccg gccatccagc ccacgaagcc accgccatcg
     7501 gcaggcccca tctccgcgcc caccgcagcg gcagccggca gcgggctctc gaaggcgagc
     7561 ggcagcgtgt ccgcacgctc gagcatcagc tccaggcgca ggctgaggcc ggaaacatcc
     7621 agcaccggca gcaggcgcag ctcctcgatg tcgcgcacga gcgcacggcg cagggtggcg
     7681 agctggccgc gatcgtggat cgccaaccgg gcctcggcct cctgcaggcc gatcagcgcg
     7741 gcctcgacgt tgcccgcgat ctgcagctgc tgagaggcca gcgcgacggc ctgctcgact
     7801 tcggcgagca cgccgtcctc gcggctgcgc gagaactcct ggtaaagcgc ctcgagcgcc
     7861 tcggcctggc cctgtgtggt ctcgaccctg gattcgagcg caccgagctt gccctgcagc
     7921 gaggcgatga cctcctgctg ctggcgcatc atcccgcgcg cctcggtggc gatggtctcg
     7981 ccatcggaga gacggctggc gacctccgcg cgcagctgtc ccgtctgcag gcgcatctcg
     8041 cgagcctgcc agatcgacca gccggccacg ccgacggcga tcagcgccag cagcagcgcc
     8101 caccacgcgg cgggactgcg agcgggctgc tcctcggcgg cgttcgccgt tcgtgcggcg
     8161 ggggcgaccg gatcgccctc gctcgcagcg ccgcgcgccg cgggatccac ctcggctgcg
     8221 ggcggtgcct cgggcacgcc cacctcctcg atgacgggtc gggtctcctc cggggccggc
     8281 gatgcggacg tcgccggcgg cggctgggtc agagcgggat tttcttgtct catggttcca
     8341 cgtctaggca aaatggcttt ctagggcctg catcaaaccg tcgtctccgg ccggcgtctc
     8401 gaccacccgt tcgaaaccgg ccgcacgcgc ctgcgcgacg atgcgcgggt gcgtggcgaa
     8461 caccgtcacg gcgcgcaggg cggcgagccc ctccgtgccg agcatgcatt ccagattgcg
     8521 tacgccctcg ctgctggtca gcagcaggcc gtcgagctcg ccccgcgccg ctggcccgag
     8581 cagcagcgcg ggatcggcct cgggacaatg gcgtcggtag caggtgacat attccacgct
     8641 cgcgccgcgt tcgcgcaagg tgtcggcgag cagttcgcgg ccgccgtcgc cacggaagat
     8701 cagcaccttg cgcccgcgca ccgcgtcggc ggcgaattcg ggcagggcga tcacggcctc
     8761 gctgtcgaaa ccgtcctgcg gcgcgatgac gttcgcgaag ccccagtcct gcatcacgcg
     8821 ctggctgccc ttgcccacgg tggcgacacg caggcgggcg ggccaggtgc gctgcggcag
     8881 gatcgcgcgc atggcgtggt cgaccgcgtt ggggctgacg aagaaggcga ggtcgaactc
     8941 gtcgaggcgg ccaacgatgg cctgcagcgg cgccggatcg agcgccggac cgaccgcgat
     9001 caccggaaag cgcagcgcac gcccgccggc ggcctcgagg cgggcgcaca ggctttcggc
     9061 ctgctcgcgc gggcgcatga cgacgagggt gcgcccggcg aggggacgca cggaccggag
     9121 atcggaagct gccgccgggg tcgtcaccgc gcgcctccct tgccgtccgc tcagccgagc
     9181 tcggcgagga tcgcttccgc gccctgcgcc cgcaggtcgt cggcgagcgc gcgcccgagc
     9241 gcctcggcct cggccggcgc gccctcgcgt tcggcgcgca cgatggtgct gccgtcgggg
     9301 cgcgcgacga agccgcgcaa ccacagccgc ccctcgcgca tcaccgcgta ggcgcccagc
     9361 ggcacctcgc agctgccggc gagcgcgcgt gcgaccgcgc gctcggcgag gacgcacgcg
     9421 gtggtgtcgg catcgacgag cggctgcagc cagccgatca cgtccgcgcg cgcggacagg
     9481 cattcgatgc cgagcgcgcc ctgcccggcc gcgggcaacg agacctcggc cggcatcacc
     9541 gagcggatgc gctcgcccag gccgagccgc gtgaggccgg cggcggcgag gatgatggcg
     9601 tcgtactggc cctcgtcgag cttgcgcagg cgggtatcga ggttgccacg caggctggtg
     9661 acgccgagga agggatactg cgcgtggatc tgcgactcgc ggcgcagcga ggaggtcccg
     9721 accacggcac ccgggggcat gtcggcgagg ctctcgtagc ggctggagac gaaggcgtcg
     9781 agcgggacct cgcgcgcggt gatgcagggc agcgcgaact ccggctccat caccatcggc
     9841 acgtccttca tcgaatgcac ggcgatgtct gcgcggccgt cgagcagcgc ggtctcgagc
     9901 tccttgacga acaggccctt gccgccgacc ttcgcgagcg gacgatcgag gatctggtcg
     9961 ccacgcgtgg tcatgccgag cagctcgacc cggcagccgg ggtggagcgc ctcgaggcgt
    10021 gccttgatgt gctcggcctg ccacagggcg agacggctct cacgggttgc gatgacgatg
    10081 cgctcggggc tgctttgact cacgatcggg attcacctgg aaaaacgggg gcacaggtgc
    10141 ggaggcgacg ggcgccagga tgatgcatcg caacatgaca gaatgctgaa tgcgtccgca
    10201 cgactgggcg cattctagca cgcgggccgg ccgcgccgcc tcccaccggg cagccggcgc
    10261 gaaagacggc agcaccgggg atcgacccga aacgggcagg aaagcgaccc gcttgcggta
    10321 agctccgctc tcccctccaa tcgaattccg aaacgaaaga atgaaccagg acaaggacgc
    10381 ccccctccgc gacgacatcc gcctgctcgg ccgcctgctc ggcgacaccg tgcgcgacca
    10441 gcagggcgag gccgcattcg gcctcatcga acgcatccgc cagaactcgg tgcgcttccg
    10501 ccgcgacgac gacatcgccg cgcgccgcga gctggaggac atcctcgacg ccttgtcacg
    10561 cgagcagacc atccaggtgg tgcgcgcctt cagctatttc tcccacctgg ccaacatcgc
    10621 cgaggaccag caccacatcc ggcgctcgcg cgcgcacctg atcgccggct cggcaccgcg
    10681 cgagggcagc ctggcgcacg cgctcgagcg cgcgctcgac agcggtgcgg ccgaccccgc
    10741 ggcgctggcc ggcttcttcg acggcgcgct ggtgtcgccg gtgctcaccg cccaccccac
    10801 cgaggtgcag aggaagagca tcctcaactg cgagaccgtg atcgcgcggc tcctggacgc
    10861 gcgcgaccgc atgcagctca cccccgagga ggccgaggcc aacgacgagg cgctgcgtcg
    10921 cgcggtgctg acgctgtggc agacgcgcat gctgcgcacc gccaagctgt cggtgatcga
    10981 tgaggtcaac aacggcctgt cctacttcga gaccaccttc ctgcgcgaac tgccacggct
    11041 ctacgccagc ctggaggacc gtctcgccgc cgccgaccgg gcgctgggca cgcccgagct
    11101 cgccgccttc gtgcaggtgg ggagctggat cggcggcgac cgcgacggca accccttcgt
    11161 caccgccgag gtgctcgagc gcgcgctcgc catgcagtgc gcggtcgcgc tcgggcatta
    11221 tctggacgag ttgcacatcc tgggctcgca gctctcgctc ggcatcggcc tggtctcggc
    11281 ctccgatgcc ctgctcgcgc ttgccgaggc ctcgcccgac cactcgccgc atcgcagcga
    11341 cgagccctac cggcgcgcga tctcgggcat ctacgcgcgc ctggccgcca cctaccgcag
    11401 cctgcttggc cacgacccgc tacgccacgc ggtcgccgcc gcccagccct acccgagcgc
    11461 ggccgcgctc gccgaggacc tcgaggtgct gcaccgctcg ctcgccgcca acggctcggg
    11521 cgcgctcacg cgcgggcggc tgcgccacct gcgccgcgcg gtgaaggtgt tcggcttcca
    11581 tctcgccccg ctcgacctgc gccagaactc ggatgtgcac gaacgcacgg tcgccgagct
    11641 gctcgaacgc gcctgccccg gcaccgccta tgccagcctc gacgaggacg cgcgctgtgc
    11701 gctgctgctc gaagaactcg ccaccgcgcg cccgctcgcc tcgccgcacg tccgctacac
    11761 ggacgagacc gagggcgagc tggcgatctt ccgcgccgcc cgccaggccc acctgcgcta
    11821 cggcacggcg gcgatccgca actgcatcat ttccaagacc gacgacgtct ccgacctgct
    11881 cgaactcgcg gtactgctga aggaggcggg cctcctgcgc ccgctcgaga acgcgctcga
    11941 cgtgaacatc gtgccgctct tcgagaccat cggcgacctc gagaacgccg caggcgtcat
    12001 ggcgcgcatc ttctcgatcc ccgcctaccg cggactgctc gaagcgcgcg accacaccca
    12061 ggaggtcatg ctcggctact ccgacagcaa caaggacggc ggcttcctga cctcgggctg
    12121 ggcgctctac aaggccgagg gcgagctggt cgaaaccttc gcgcgccacg gcgtgcgcct
    12181 gcgcctcttc cacggccgcg ggggctcggt gggccgcggc ggcggcccga gctaccaggc
    12241 catcctcgcc cagcccgagg gcgcggtgca ggggcagatc cgcctcaccg aacagggcga
    12301 ggtgatcggc gccaaatatg gcaatccgga agtcggccga cgcaacctcg aggtgctggt
    12361 cgccgccacg ctcgagtcca gcctgcgccc ggccagcgcc agcccgacgc cgcccgcctt
    12421 cctcgacgcc atgcaggcgc tctcggacgc ggccttcgcg gcctaccggg gcctggtcta
    12481 cgagaccgag ggtttcgagc gctacttctg ggagtcgacc gtgatcagcg agatcgcggc
    12541 gctcaatatc ggcagccgcc cggcctcgcg caagaagagc accgcgatcg aggacctgcg
    12601 cgcgatcccc tgggtgttca gctggtcgca gtgccgggtg atgctgccgg gctggtacgg
    12661 cttcggcagc gcggtgcgcg atttcctcac cgccaacccg gacgacggcc tggcgctctt
    12721 gcagcgcatg aacatggaat ggcccttctt ccagaccctg ctgtcgaaca tggacatggt
    12781 gctgtccaag accgacctcg cgatcgccag ccgctacgcc gagctggtca aggacgcggc
    12841 gctgcgcgac acgatcttcg ggcgcatccg cgccgagcac caggccacgg tcggggcggt
    12901 gctgcggatc accggccaga gcgagctgct cgacgccaac ccgctgctca agcgctcgat
    12961 ccgcaaccgc ttcccctacc tcgacccgct caaccacgtc caggtcgagc tgctgcgccg
    13021 ccaccgcgac gccgccgagg cgggcggcat cgacgagcgc atccggctcg gcatccacat
    13081 ctccatcaac ggcatcgccg cgggcttgcg caacagcggc tgagccgtct tacaggccga
    13141 gcgcctgccg caccgccggc cactgccgcc ggctcaccgg cagacgctcg ggcagatcgc
    13201 gcaacagcac ctcccagcgc ggctcggcct ccttctgtcc atcccgctcc gctgcccgct
    13261 ccacacccgc caccgcggcg agcgcgacca ggcagttgcg gtgcaggcgc aggaaactgc
    13321 gcggaaactc ctgctcgatc tgcaccagcg actcgtcgag cacatactcg cgcgccgcgg
    13381 tgcgcgcggt gacgtacttg agctcggcac gcagatagcg cacctcggcc accggcacca
    13441 gcagaatgcg gccgcactcc gcgacactga agtggcgacg cgcgcgcgtc gccagcgtgg
    13501 ccagcacgtc ggccgccggc ggcgcgcggc gcgcgcgtac gcgctcgagc gccgcggcca
    13561 ggcgctgcgc gcgcaccggc ttgagcaggt aatccaccgc ggcgagctcg aaggcctgca
    13621 ccgcgtactg gtcgtaggcg gtcacgaaga ccagcgccgg aggcgccggc aggcgggcga
    13681 ggtggcgggc gagctccacg ccgtccatca ccggcatgcg gatgtcggcc agcaccacgt
    13741 cggccgccgc ggcgtcgagc agtcgcaggg cctcgacgcc gttggcggcc atgccgacga
    13801 tcgcgttggg ctgctgcccg gcgacgtcgc cgagcaggtc gcgcaggcgc gcacgcgccg
    13861 gcgcttcgtc atcgacgacg agcacgcgca gcgccgcgac agcctcgggt gcggttgcgg
    13921 tcatgcggcc tccctctccc agggcaggcg gatgcgcacc cggtagcggc catccgcctc
    13981 gtcgatctcc agcctcgcct cgaggtcgtg gaacagcatc aggcgctcgc ggatgttatc
    14041 cagcgccatg cggttgccga catggtgcag cccctgcgcg gcgaccgggt tgtccacctc
    14101 gatgcaaagc tccttgccgt ggcgcgcgat gcggacgatc acctcgcccg cggcggcggc
    14161 aggctcgatg ccgtggtaga cggcattttc gagcaggggt tgaagcagca gcgaaggcat
    14221 gcgggcgcgc agcagttccg cgggcacgcg cgtacgcctc tcgccgcctt caccacctcg
    14281 tccgccctcc ccgccttccc cgccttcccc gtcctctccc gcctctccac cccctcctcc
    14341 aaactcccag cgcaccgcga ggcgctcgcc gagccgcagg cgctccagcc cgatgtagcg
    14401 ctcggcgagc tcgatctcct cggccagcgt cacgcgctcg cggccgtcct gcatcagcgc
    14461 gcggaacagg tcggcgagct cctccagcgc ctgttccgcg cggcgcggct cggagcggat
    14521 gacgccgagc acgccgttca ggcagttgaa gaagaagtgc ggccggatgc gcgcggtcag
    14581 tgcgatcagc cgcgcctcgg cgagcgccgg cgacaggcgc cggctgcgcc agtcgaaata
    14641 cgccaggcag agcgcagcgg cgagccacgc ccagcccagt ggtcgccacc actccgcggc
    14701 cggcaccgcg gcgagcaggc tggcggcgat cgccgccagg ctggcgagca cgagtacgac
    14761 gccgcgcccg gccgcgaccg gcagcgcact cagcccgggc gcgccgaggt agaggagcac
    14821 caggctcagc agcagcggca gctccacccg cccggccatc agcgcgagct cgtcggcgag
    14881 ccgggcgggc gcatcgagcc ggatcgccac ggtgagcgcg gcgaagaggt tcaccgccag
    14941 caggacgcgc aacatcaccc cgaggttgcg gaagtcgggc aggcccggcg cgccgagcgc
    15001 cgggggcttt tgctttatac tttcggccat ctacgcgcgc caccgcaggc ggcgcccctt
    15061 cccgtaatcg ttccatgccg ggccggcagc gcagggccgg ccttttccgt ttccaacacc
    15121 ccgattatga cagacaccac gacgccggcc gccagcgcca acgcccccgc cgacgcgagc
    15181 gccaaggcct ggtccggccg cttctccgaa cccgtttccg aactcgtcaa gcgctacacg
    15241 gcgagcgtgt ccttcgacca gcgcatggcc gcgcaggaca tccgcggctc gctcgcgcac
    15301 gccaggatgc tcgcccgcca gggcatcatc ggcacgcagg atctcgtcga catcgagcgc
    15361 gggatggcac agatcgccgg cgagatcgag cgcggcgaat ttgcctggag cctcgacgac
    15421 gaggacgtgc acctgaacat cgagaagcgc ctcaccgcgc tggtgggcga cgcgggaaaa
    15481 cgcctgcaca ccggccgcag ccgcaacgac caggtcgcca ccgacatccg cctgtggctg
    15541 cgcgacgcga tcgaccgcat cctcgggctg atcgccgagt tccagtcccg cctgctcgag
    15601 gtcgccgaaa cccatgccgg cacgcccctg cccggcttca cccacctgca ggtggcgcag
    15661 ccggtcacct tcggccacca cctgatggcc tacttcgaga tgagccggcg cgacgccgag
    15721 cgcttcgccg actgccgcaa gcgcgtcaat cgcctgccgc tcggctcggc cgcgctcgcc
    15781 ggcaccagct acccgatcga ccgcgagttc gtcgcttccg agctcggctt cgacgaggtc
    15841 tgctacaact cgctcgacgc cgtcagcgac cgcgacttcg cgatcgagtt ctgcgccgcc
    15901 agcgcgctgc tgatgaccca cctgtcgcgc ctgtccgagg agctgatcct gtggatgagc
    15961 ccgcgcgtgg gcttcatcga cctcgccgac cgcttctgca ccggcagctc gatcatgccg
    16021 cagaagaaga accccgacgt gcccgagctg gtgcgcggca agaccggccg cgtcaatggc
    16081 agcctggtcg ccctgctcac gctgatgaag ggccagccgc tggcgtacaa caaggacaac
    16141 caggaagaca aggagccctt gttcgacacc gccgacacgg tcatcgacac gcttcgcatc
    16201 tacgccgaca tgatcggcgg catccgcgtc aaggcagagg ccatgcgcgg cgcgctcagc
    16261 cagggctatg ccaccgccac cgacctcgcc gactacctgg tgaagaaggg cctgcccttc
    16321 cgcgacgccc acgaggccgt ggcgctggcg gtgcgcgcag ccgaggtcaa gggctgcgac
    16381 ctgccgcagt tcaccctcga cgagctgcgc atcgccatgg cccacgtgcc cggcgccgcc
    16441 gagcgcctcg gtgaggacgt cttcggcgtc ctcaccgtcg aaggctcgct ggcctcgcgc
    16501 aagcacatcg gcggcaccgc gcccgagcag gtgcgcgccg cggtggcgcg cgcccgcatc
    16561 ggcctcggcc tcggccaagg ctgagcccga tccgacgccc atgaccacag cggcggccga
    16621 gcgcatcggc aagtacgagc ttctccgccg catcggcaag ggcgccacca gcagcgtcca
    16681 tctcgcctgg gacccgttcc gccaggtcga ggtggcgatc aagcgcctgc gccgcgagat
    16741 cctcgccgat ccgcagcgcg gccgcctgct gcgtcagctc ttcctcaacg aggccgcgct
    16801 cgccggcaag ctcgcccacc cgcacatcgt gcgcatccac gatgcgctgg tcaccgacga
    16861 ggatgcctgg ctggtgatgg accacgtgcc cggcggcacg ctcgaggaca tcgcgacgcc
    16921 ggcgacgctg gcccccttcg ggcgcgtcat cgagatcgtc ttcaaggcca cgcgcgcgct
    16981 cgactacgcc ttccaccagg gcatcacgca ccgcgacatc aagccggcca acatcctgct
    17041 gatggcgccc ggcggcagcg acatccgcat ctccgacttc ggcgccgcgc tgcacaccgc
    17101 gcgcgagacc acgcagatcg ccggcgtcgg ctcgcccgcc tacatgtcgc cgcagcaggt
    17161 gcgcgaggtg ccgctcgacc accgcaccga catctactcg ctcggcgtgg tgatgttcca
    17221 gctcctcacc ggccacctgc ccttcgagca ggagaacagc tacagcctgc tctacagcat
    17281 cgcacacgac accccgccgc cgccctccga gctgcgcccc gaggtacccg aggcgctcga
    17341 cgcgatcgtg cgccgcgcca ccgagaagga catcgagcgc cgctaccaga gctggagcga
    17401 gttctcccac gacctcgcgc tcgccttccg caaccgcagc ctcgcgctcg ccccggaccg
    17461 cctcggcgac accggcaagt tcgaggcgct gcgcacgatg cgcttcttcc gccgcttcgc
    17521 cgacgccgag ctgtgggagg tgatcgggct gtcgcgcttc gaccgcgcgc tcgcgggcac
    17581 ggtgatcatc aaggagggcg agcgcggcga cgccatctgc ttcgtgctcg ccggcgaggc
    17641 gcgggtgagc aagcagggac gcctgctcga ggtgctcaac ccgggcgact gcttcggcga
    17701 gatcgcggtg ttctccacca tctccggcgt gcgctcggcc tcggtcgagg ccgccgcgga
    17761 caccgagctg gtgacgattc gcgcgcgcgc gctcgagcgc gccagcgaga ccctgcgcat
    17821 gcatttctac caggctttcc tcgaggtgct ggcgacgcgc ctgtcgcaag ccagtgcccg
    17881 catcgccaat ctatgaacga atcgccgacg ccccaccccg cccttcccgc cgtcgaggcc
    17941 gccctgcgcg ccgccatggg cgagcagctg cgcatcgtct ccgccgagcc ggtgagcggc
    18001 ggcagcatcc acaccgcgct gcgcctccag gacgccggtg gcgcacgttt cttcgccaag
    18061 ctcgcccctg ccgcccaggc gccgatgttc gaggccgagg ccgacggcct cggcgcgatc
    18121 gcggcaagcg gcagcctacg caccccggcg gtgatcgcgc gcggggggga cgaggcccac
    18181 gccatcctgg tgctggaatg gctcgacctg cagccgctga ccagcgcggc cgacggcgcc
    18241 cgcttcgccg aggcgctggc ggcgctgcac cgcaacgtcg gcgagcactt cggctggccg
    18301 cgcgacaact tcatcgggcg cacgccgcag gcgaacaccg agcgcgacaa ctgggcgcgc
    18361 ttcttcgtcg agcagcgcct gcgtccgcag ttcgcgcgcg cccgcgcgca gggcttcgac
    18421 atcgagctgc agcgccaggc cgaccgcctt ctcgaccgtg tccccgcgct tttcctcgac
    18481 taccgcccgc ccgagagcct gctccacggc gacctctggc acggcaacgc ggcggtgctc
    18541 gccgacggca cgccggtggt gttcgatccc gcggtgcacc gcggcgaccg cgaagccgac
    18601 ctcgccatga gcgagctctt cggtggtttc ccctccgcct tctacaccgc ctaccggcgc
    18661 gcctggccgc tgcacgagga ctacgagcag cgcaagccgc tgtacagcct ctatcacctg
    18721 ctcaaccacc tcaacctgtt cggccgcgcc tacctgcgcg agtcccttcg gctggccacg
    18781 cggctgaacg aggagttggc gcggcgcagg gactgaaacg ccccgaagca ggagcggatg
    18841 cgtcggcgtt ttttacactc ggccccggag cgcgatctgc ccgcacagca cacctatttt
    18901 gaaatcagcg acttgcgtcg atttttcgac gcgggcgtga tatttgctac ggtctgggta
    18961 aggttcatcg gaaagacgtc gggcggcaga tataatgaaa tcaggcacca gcggaatcag
    19021 ggattgatga tggacaggat ccggaacaac gagacggtcg agcagcgaag gcgagtcctg
    19081 aagggcgcgc tgggcgcgtc caccgtgctc acgctgggat acggcggtag cgcggctgcg
    19141 gcatcgatcg gatgcgtgaa gaacacgtac attgaagaaa ccccgcctgc gaacggtaac
    19201 cagttttcgt ggaccgaccc ggcggccatg accggggttg acgagtgggc ctggagcaag
    19261 gtcgacgtgt gggaatgcag cgggaacgtg gggggcgttc cggatacgga cttcgagggc
    19321 ttctcgctcg acaacggcat caattggtac cgcgtgccgg cggtggtcgg cgaagctccg
    19381 acgctgatcg ccttggcgac gaagaagagc tcgcagtcgc ccgcgtaccc gaagaccgct
    19441 tgggttttgg cgtacttcga cgcgaacggc aacttcgtcg gcaactaccc cgcgatcacc
    19501 atgcgcaccg agcagaatac cccgctggag ggttcctgcc tcgcgtcggt gaatccgaat
    19561 gcgaagagtg gcttcaccta cggcggctga acgccgatgc agccgacctc gccgctgggg
    19621 ccgctggcct ggatcgaacg ctactgcccg tccctcgacg ggcagtttct ttttctcgat
    19681 ccgctgcgct gggataccca tctgctgagc gcgggcgccg tgatcgtgct gcgcgaggcg
    19741 gcgctcgcca tcgaagccgg atgtttcgag gccttccgcg ccgaggtcgc cgcgaacggc
    19801 ggctggccgg cgggactgga acgcctggcg gtcgcgctga ccgcgctcgc cgagcgcgct
    19861 gcgggcacgg ggaccgaggc ttgaagggcg gcgtgatgca gcgctcgatc gacctcggtg
    19921 cggccagcgt ggtggtgcgc tcgccgcttc gcgagttcaa tgcccagctc gacctgctct
    19981 accgcgacca ttcgcaggca ggggacagcg gcttcgcgga cgtgcgcgtc gcgctgctcc
    20041 ccgggccgcc ctggcgcgtg tggggcccgc aggtacgcct gtggtcggag gcctcctgcc
    20101 ccttcgaccc ctacccgctc gacagcgccc tgcctctttt cgaatggggc accaactggc
    20161 tgctggcgca gcgcctcaac gcgcatgtgc tgctgcatgc aggcgtggtc gcgcgcgagg
    20221 accgtgccct gatccttccc gccgcaccgg gctcgggcaa gagcacgctg acctgcgccc
    20281 ttcacctcgc aggctggcgc ttcctctcgg acgagttcgg ggtgctggat accgaatccg
    20341 gactgctgca ccccctgctc aagcccgcag cgctgaagaa tcgctcgatc gacatcatcg
    20401 gcggccagcc gggcgcggtc ctcggccccg tctttgccgg tacgcgcaag ggcgacgtgg
    20461 cgcacttcgc acccgaccgt gcgagcgtcg cagcacgccg ccggccggtc acacccgcgc
    20521 tcgtggtgtt cccgagatat cgcgaaggcg cagccctgcg ctgcgaaccg gtgcgccggg
    20581 cggaggccgc gatgcggctg gggctcaaca gtttcaatta ccgcaagctc ggcccggtcg
    20641 gcttcgacgc catcctccgc ctcgcgcgca cggcccccgc ctgggagatc gagtatggcg
    20701 cgctcgccga cgccctcccc tgcatcgagc agctattcgg aaaagcctca tgagcacgca
    20761 tccggcggcc gactgtgccc tgctgctgcg cggccttcac gaagccctcg agatcccgga
    20821 ggcgcgctgg gatcgggtgc tgcggctcgc gcgcatgacc ggcctgcatg gccgcctcgc
    20881 cgccgccaac gcggacaatc cgaccgtgtc ggagcgggtc cgccgccatc tgctgtcggc
    20941 gacgaggatg gcggacttca gcgcgcgcat gctcgacgcc gagctgcgcg acgtggcccc
    21001 gctgtgcggc ggcgatttcc cggtgatcgc cctgaagggc ggcgcctacg cactgcaggc
    21061 gctcggcttc gcccgcggcc gcttcgtctc cgacatcgac ctgctggtgc ccgcacgcgc
    21121 cctgcgcacc atggaggcga ggctgcacgc cgcgggctgg gaaagcgcgg cgctggaccc
    21181 ctacgatgaa cgctactacc gcgactggag ccacgagacg ccccccctgc gcttcccggg
    21241 ccatttcctc gaactcgacc tgcatcacgc gatcacgccg gtgaccgggc ggcgccattt
    21301 cgatccggcg ccgctgttcg acgagagctt gccgatcgcc ggcagccgct tccgcgtgct
    21361 gtcggcgcgg gaccaggtgc tgcatgcctg cctgcattgc ttccaggacg gcgatctcgc
    21421 cctgcgcgtg cgcgagatcg ccgacatcga cggtctgctg cgcagcttcg ccggcacgac
    21481 gggcttctgg ccgggcctcg tcgagcgtgc caatgcgctc ggactgggcc gtccgctctg
    21541 gtatgggctg catttcgcgc gcagctggat gcagacaccg gtccccgagg cggtcctgag
    21601 cgcccttccc ggccccgccg ccgtactccg ccggccgatg gaggcctgca tctcgctcgc
    21661 cctgctcccg cccgaccccg atcggccacc cggcctgtcg gtgcgattcg cgcggcggct
    21721 ggcactcgcg cgctatcacc tgctgcgcat gcccctgcac atgctcctgc cccacctcgc
    21781 gcgcaaggcc tggttgcgca cacgccgccg cacacccgac ggtgcgaatg aaaacgccgg
    21841 cggcggaccc tgaaaacggg tcggcagccg gcgttccacg acgaaggtcg tcgcgcggga
    21901 tcagtcctcg ccgacgagtc cggcgtcctt gagcatctgc tgcagttcgc cgctgtcgta
    21961 catctcgcgc atgatgtcgc tgccaccgac gaattcgccc ttgacgtaga gctgcgggat
    22021 ggtgggccag ttggagaatt ccttgatgcc ctgacggatc tcggggtcgg caagcacgtt
    22081 gaccgcgagc acctcgcgca cgccgcagcc cttgaggatc tgcaccgcgg tggaggagaa
    22141 gccgcactgc gggaactggg gcgtgccctt catgtagagc accacgccgt tgttggtgac
    22201 ctgatcgcgg atgacgtcct ggatgtccat tctggaaacc tgaaaaagtt gagttaaaca
    22261 gtcgggaaat aataggcgtt gcgcgccgcc agcgtcaacg cgaatcgcgc gttgcgcgcc
    22321 ccagccagcg cccccccgag acgcgctcga tgccggcgag gtcggcccac gaggcgatcg
    22381 cggcaaagcc ggcgtcggcg agcaggccgc gcgccgacgc cgcctggtcg tagccgtgct
    22441 cgaggaagag ccagccgcca tcgaccaggc gtgccggcgc gccggcgacg atctcggcga
    22501 ggtcgtccag gccctgcggc ccggcggcga gcgcgccacg cggctcgaag cgcacgtcgc
    22561 cctgctcgag gtgcgggtcg cccgcagcca cgtagggcgg attggacacg atgagctcga
    22621 agtgctcgtc gccgagcgcg ccgaaccagt cgctctgcac gaaggagacg ctcgcgccca
    22681 gcctggcggc gttggccatc gccacccaca gcgcctcgcg cgagcggtcg agcgcgacca
    22741 cctcggccgc ctcgagctcg agcgccaggg tcaccgcgag cgccccgctg cccgtgccga
    22801 ggtcgagcac gcgcagcccg cgccgtccgg gaaagtgcgc gagcgcgagc tcgaccagca
    22861 gctcggtctc ggggcgcggg atcagcaccg ccggggtgac gaggaactcg cgcccgtaga
    22921 actcgcgcgt gccggtgaga taggccaccg gctcgccggc ggcgcggcgc tcgacgaggg
    22981 cgcggtactc ggcccagtcc tccgcagcaa gcttctgctc cggccacgcc accagacgcg
    23041 cggccgggca ctgcagcacg tggcgcagca gcacgcgcgc gtccatctgg tcgatgcgcg
    23101 cgcgtgccca ggcgagcgcg ccggcgatgt cgatcccgtc ggcagacggc gcggcgggac
    23161 tcatgcatcc tccccgagtg ccgcgagcag gtcggcctgg tgctcgaggg tgagcgcgtc
    23221 gaagatctcg tcgagctcgc cctgcatcac cgcatcgagc ttgtagaggg tgaggttgat
    23281 gcggtggtcg gtcacccgcc cctgcgggaa gttgtaggtg cggatgcgct cggaacggtc
    23341 gccgctgccg accaggctct tgcgctgcgc ggcctcggcg ctctgttgcg cgcgcaccct
    23401 ggcgtcgtac aggcgcgcgg cgagcaccgc catggcctgc gccttgttgc ggtgctgcga
    23461 gcgatcgtcc tggcactcga cgacgatgcc ggtgggaatg tgcgtgatgc gcaccgcgga
    23521 gtccgtcttg ttgatgtgct gcccgcccgc gcccgaggcg cggaaggtgt cgatgcgcag
    23581 gtcggcgggg ttgatgtcca ccgcctcgac ctcgtcggcc tcgggcagca cggcgaccgt
    23641 gcaggcggag gtgtggatgc gcccttgggt ctcggtcacc gggacacgct gcacgcggtg
    23701 gccgccggat tcgaacttga gcttcgaata ggcgccgttg ccgaccacgc gggcgatgac
    23761 ctccttgtag ccgccgaggt cggattcgct cgccgacacg atctccacct tccagcgctg
    23821 gcgttcggcg tagcgggtgt acatgcgcag gaggtcgccg gcgaacagcg ctgcctcgtc
    23881 gccgccggtg ccggcgcgga tctcgaggaa caggttgcgc gcgtcgttgg gatcgcgcgg
    23941 cagcagcgcg cgctgcagct cggcctccag cgcttcgatg cgcgcctcgc cctcggcaag
    24001 ctcgagttcg ccgagctcac gcatctcggg gtcggcgagc agctcgcgcg cggtcgcgca
    24061 gtcggcctcg gcctggcgat gctcgcggta cagcgccacc accggctcga tctcggcgcg
    24121 ctcgcgcgag agctcgcgga aggcgttcat gtcgcgcgcg ccctcctcgg aggcgagcgt
    24181 gcggtcgagc tcttcgagcc ggttggcgag gtgttcgagt ttgtcgcgga tgctctgctt
    24241 catgacggag gaaggacgcg cacacgccgc cggcgggcgg cggcctgcgg agccgtgcgg
    24301 ctagtgggaa tcgcgggaca ggttgaagag ctcgcgcacc acgcggccgg cttcgacctt
    24361 gtgctcgccc tcggcctggt tcaggaagcg ggtggggccg tgcatcagct tgttggtgag
    24421 gccgtgcgac agcgcctcga gcacgcgctg cgggtcctcg cccttggcga gcaggcgcac
    24481 ggcgcgctcg agctcggcct ggcgcaggct ctcggcgtgc tcgcgcagcg aacggatcac
    24541 cggcaccgtc tcgcgcgccg acatccagtg caggaagcca tcgacccggc tggcgatgat
    24601 ctgctcggcc tcgatcaccg cctgctggcg cgactccatg ccggcctcca ccacctgggc
    24661 gagatcatcc acggtgtaca ggaagatgtc gtcgagttcg gcgatctcgg gctcgatgtc
    24721 gcgcggcacg gccagatcga ccatcaccat cgggcgatgg cggcgcgcct tgaccgcgcg
    24781 ctcgaccatg cccaagccga cgatgggcag cggcgcggcc gtacaggaca cgacgacgtc
    24841 gaaacgcggc agcgcctcgc caatctgatc gatgcgcatg gtggtggcgc cgaagcggtc
    24901 cgccagggcc ctggcacgct cctcggtacg gttagccacc gccatggatt tgggccgagc
    24961 gccgccgaag tgggcggcac agagctcgat catctcgccc gcgccgatga agagcacgtg
    25021 ctggtcggca acgcgctcga agatgcgctc ggtcagatgc acggccgcag cggccatcga
    25081 caccgtgttg gcgccgatcg cggtggtcga gcgcacctcc ttggccaccg cgaaggtgtt
    25141 ctggaacagc ttgtgcaaca gcacgcccag ggtgcccgcc tcttcggcgt agcgcgcggc
    25201 gtccttgacc tggccgagga tctgcggctc gccgatcacc atcgagtcga gcccgctcgc
    25261 cacccggaag acatggcgga tcgcgtcgcg ctgcgggtag gaatacaggt agggcgagac
    25321 ctcggcgagc gcgaggcggt ggaaacgagc gagccagtcg gccgcgtgct ggggctgctc
    25381 ggcggcgaaa tagagctcgg tgcggttgca ggtcgacagg atcgccgcct cgcgcaccga
    25441 atccgtatgg gccagatcgt gcagcgcctg ccccatgcgc tcgggctgga acgcgacgcg
    25501 ttcgcggatg acgagcggcg cggtgtggtg gttcaagccg agggcgaaga gttgcatgcg
    25561 ggaggggggt gggacggagc gagccggatt ataacaccgc gcctgcgccc caaaattgag
    25621 ccggaacagc gacttctggc cgcaaattga cgccgcaaag caaatgggcc gacactcggt
    25681 cggcccatcg ggatt
//
LOCUS       DAOQBS010000033        25025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1255_length_25025_cov_16.494233, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000033 DAOQBS010000000
VERSION     DAOQBS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 25025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25025
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1255_length_25025_cov_16.494233"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PKN25_05765"
     CDS             <1..782
                     /locus_tag="PKN25_05765"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945370.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HNV90442.1"
                     /translation="NLHKTFCIPHGGGGPGMGPIGLAAHLAPFMADHVIQPTGGAGRP
                     NLGQGAVSAAPFGSASILPISWMYITMMGGEGLKQATEVAILNANYLAARLKDHYPVL
                     YTGSKGRVAHECILDIRPIKATTGISEVDIAKRLMDYGFHAPTMSFPVAGTIMVEPTE
                     SEDLGELERFVAAMIAIRDEIRQIEAGNWPAEDNPLKHAPHTQADFLGDWKRPYSREQ
                     AVFPLPWVAENKFWPSVNRIDDVYGDRNLFCACVPMSDYAE"
     gene            931..2553
                     /locus_tag="PKN25_05770"
     CDS             931..2553
                     /locus_tag="PKN25_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-glucan synthase"
                     /protein_id="HNV90443.1"
                     /translation="MIFAPRTNRSQLWLALLIASTTMLLLLAAMLWQQTRPVRMHDLH
                     LAEGEKLRCVSYAPYHRPGQTPLLEDTRIEREQIAADLAALAKITDCVRLYSIDQGLE
                     HVPELARPLGLKVLLGAWIGYEKTKNARELERAIALANANADVVRALIVGNEVLLRRE
                     RTEDEMRELIRHAKAHAKVPVTYADVWEFWTRHDSLANEVDFVTVHILPFWEDEPVDI
                     ELALQHVAETRRHVGEHFAGKKILIGETGWPSAGRQREESKPSRVNQARYIREFVHRA
                     HAEGWDYNLIEAIDQPWKRRLEGTVGGFWGMLDADSLEPKFPLAGAVAEREGVAGPVG
                     GAAVGGVLAFLLATTGRRTRCLRASAIAAAGTLGGMITVLHWEHAHLAYRDALEWSLL
                     GGVAALGALLPLTLARWHGEPVPATGRAARILRHGEPLRAKAGALGLLRGLLLFAAAV
                     AALLLWVDPRYRDFPTLLYLVPAVVLGVVGWWRADTTRSEITLALVILIGVVARWSSE
                     PANPQAVAWLLTGLALALPVLLVRSHQHEQRE"
     gene            complement(2446..2880)
                     /locus_tag="PKN25_05775"
     CDS             complement(2446..2880)
                     /locus_tag="PKN25_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90444.1"
                     /translation="MPTNLLKYGVARPLLAGLALAALALWLRHGVLEAGVLPRDCTAA
                     DAPALACLFKQAFVHSFLDQRIGWVSLVAGVLAFVRASRGLAWAGWLCGVAGLVLYSY
                     DPAAVGALLALLVLVRAHQQHGQGQREPGEQPGDRLGVGRLA"
     gene            3185..5791
                     /locus_tag="PKN25_05780"
     CDS             3185..5791
                     /locus_tag="PKN25_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV90445.1"
                     /translation="MKNAASFAIRILTAIAIAAVVAFAQYWIWQQLNRSTEFIGTNQS
                     IKGFAYNGFQRDQSPLKGTYPTRAELASDLDLLGRYSDGLRTYGVNDMPELLDLAGER
                     DMLVTAGAWIDARPESNAREVAALIEAARKMRHIERVMVGNEAILRGDVTVDELIVYL
                     DEVRKAIRKPVSTAEPWHVWLRYPELAKHVDYITVHLLPYHEGLPVDKAVEYAFQRYD
                     EVARAHPRKKIVVGEVGWPSRGPTIDAAIPSLDNQARFVREFLAHPRTARIDYFLMEA
                     IDQPWKVDVEGWAGPYWGMFNADREPKYQLEGVVERDPHWSHKASNAAALAFIPMMLA
                     AFFLPGWSIGGRLFLAALIQACISTLIIGINVPVEYYLTQRDLIGLVLLIGATCMTAA
                     VLLSHGFEFGEVLFKKKWARRFTPLPPHPPEQQPFVSIHLACYNEPPEMVIATIDSLA
                     QMNYQNFEVLILDNNTRDEALWKPLERRCAELGPRFRFFHLANWPGFKAGALNYGLKV
                     TDPRAEVVGVVDADYVVDPDWLACLVPHFDQPEVAVVQAPQAHRDWEGQPFKRMCNWE
                     FDGFFRIGMHHRNERNALIQHGTMTMVRRRALEEVGGWSEWCICEDTELGLRLIEKGY
                     DTRYIDHILGRGLTPSGFAAIKSQRFRWAFGAMQILKAHLPHMIGRSTLNLAQRYHFL
                     TGWFAWLGDALQLVFAFGSLLWTLGILLFPKAFGLPVVSLALPIFGFMIFKAALGPIL
                     YRRTMDCPWKDILGASILSVGLAHAIARGVFAGLVKKKGVFVVTPKGWRGGGALAFFN
                     PIREEIGMLVALLMGAVTLVAQRGANNLETQLWVGILCLQCIPYVAAILCQVAAYMPE
                     RGAAAGETAAAA"
     gene            complement(5907..7235)
                     /locus_tag="PKN25_05785"
     CDS             complement(5907..7235)
                     /locus_tag="PKN25_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HNV90446.1"
                     /translation="MRNLNLVVAGVLFLGLLWGLEGLPDRAGAGLALLATIAWLWLTE
                     ALHVSVTALLVPLLAAASGVLDFPEALLQFADPVVFLFLGGFALAAGLQRQGLDRWMA
                     GQVLRRAGASFGRAVRWLFWLTAFLSMWISNTATAAMMLPLALGLLAPLPLDEHRRTW
                     MYVLLGVAFSANIGGIGTLVGSPPNAIAAAAVGMDFAEWMSWGLPLVLVMMPLMEIGL
                     RLVLRPRLDVAVTPPPPPGGWTPAQRKMLALFGFTVLLWVFGAPLGAALGFAQGYDAA
                     VALFALVLLHGLRLAGWQDVERSTEWGVLLLFGGGLTLSKALAASGAAAWLATQLGGP
                     LAALPAWGCIALMVLFALLLTEVTSNTASAALLIPLFLGLAPHVERAPLAVLVAVGTS
                     CAFMLPVATPPNAIVFGTGHVPQRVMILGGTGVTVLVYPALVLAALIAVA"
     gene            complement(7316..8569)
                     /locus_tag="PKN25_05790"
     CDS             complement(7316..8569)
                     /locus_tag="PKN25_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNV90447.1"
                     /translation="MRQQNLLYAHSGGVTAVINASAAAVVAAARASGRVGKVFAAWRG
                     IVGVLEEALIDTAGLDEAALVRLARTPGGAFGSCRFDLPHDDGGAAMDRLFAVFDAHD
                     IGWLLYNGGNGSMDAVARIQAEAARRGHALVCVGVPKTVDNDIVGTDCCPGFGSAARY
                     LATSMLEAGLDTASMVGSKGSVFVMEVMGRNTGWLAAATALAAQGDPDRAPHIVLVPE
                     ARFDEAAFLARVREIVERLGYCAVTVAEGIRRPDGSLLMEKSRDARGYVQLGGAGLAV
                     ARRINERLGYKFHWAIPDYLQRSAGHWLSATDRAQAEAVGRVAVEYALAGQGGTMPAI
                     RRLQDAPYRWDVVAVDTAAVANLERSLPDGFVRADGLHVTTAACDYMRPLIEGEIPPP
                     VAGGLPDYGRWDWAPVARRLAAWQA"
     gene            complement(8708..9361)
                     /gene="adk"
                     /locus_tag="PKN25_05795"
     CDS             complement(8708..9361)
                     /gene="adk"
                     /locus_tag="PKN25_05795"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNV90448.1"
                     /translation="MRLILLGPPGAGKGTQANFIKEKFGIPQISTGDMLRAAVKAGTP
                     LGLEAKKVMDAGGLVSDDIIIGLVKDRLQQEDCKSGYMFDGFPRTIPQADAMKDAGVP
                     IDFVLEIDVPDAEIVERMSGRRAHLASGRTYHVKYNPPKVAGKDDVTGEDLVQRDDDK
                     EETVKKRLDVYHSQTKPLVEYYSKWAASGDAKAPKVRKISGLGAVDAITANAFAALK"
     gene            complement(9449..10234)
                     /gene="kdsB"
                     /locus_tag="PKN25_05800"
     CDS             complement(9449..10234)
                     /gene="kdsB"
                     /locus_tag="PKN25_05800"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNV90449.1"
                     /translation="MSGGMSTDFRIVVPARYASTRLPAKPLADIGGKPMIVRVLERVR
                     HAGASETWVATDHEGVREVVQAAGGAVVMTRPDHPSGTDRLAEVALARGWADEDIVVN
                     VQGDEPLIDPEIVAAVARALAADGEAAIATAAHAIHDPVEVFNPNVVKVVCDAAGRAL
                     YFSRAPIPWARDAWGDGARDLPAGLPVLRHVGLYAYRVGFLRRYAALAPSPLEHWEAL
                     EQLRALWHGYRIRVLELAQAPAAGVDTQEDLERVRAIFAAGGV"
     gene            complement(10231..10422)
                     /locus_tag="PKN25_05805"
     CDS             complement(10231..10422)
                     /locus_tag="PKN25_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNV90450.1"
                     /translation="MDARLLEILVCPLCKGPLDYLKDKQELVCKADRLAYPIRDGIPV
                     MLDEEARAMSAEEVDALRK"
     gene            complement(10403..11410)
                     /gene="lpxK"
                     /locus_tag="PKN25_05810"
     CDS             complement(10403..11410)
                     /gene="lpxK"
                     /locus_tag="PKN25_05810"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNV90451.1"
                     /translation="MAAQAPAFWQRRGLLAQALRPLSLLFGFLARRRRALTQPQRLPV
                     PVIVVGNVAVGGSGKTPVVDWLAGVLRVAGHHPGIVSRGHGGTASAQGGLALVPADGD
                     PGRYGDEPVLLARLTACPLVVGQDRPAAARALLRLHPECDVLIADDGMQHYHLARDLE
                     IAVVDAATLGNRLLLPAGPLREPLARLAEVDLVLAHGELDAELRAAIGGVPVFPMRLF
                     GEEVVALADPACRLPLAALRGRRVHAVAGIGRPQRFFDQLAAAGLEVVAHAFPDHHRF
                     VAADLVFGDASPILMTAKDAVKCRAFAPADCWEYPVRAQIGSGAAERILEKLEDGRTS
                     A"
     gene            complement(11445..11870)
                     /locus_tag="PKN25_05815"
     CDS             complement(11445..11870)
                     /locus_tag="PKN25_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNV90452.1"
                     /translation="MNFRRGSRNGEPEINLIPLIDVVLVIIIFLMLTTTFSKVSGLEI
                     KLPTAEAEGSETEAREIVVAVTAAGEVLVNRQAVGERSVEAIAAALGKSAPADGDTPP
                     VVVINADARTPHQSVVDVMQAAQRAGLPHITFATQSSAQ"
     gene            complement(11881..12489)
                     /locus_tag="PKN25_05820"
     CDS             complement(11881..12489)
                     /locus_tag="PKN25_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNV90453.1"
                     /translation="MFAIIQAVGWPIWPLILASVIAVALIIERSITLRRSRIVPPGLL
                     DKVLDDLRQIGPSAEMAERVAAHSPLGRVLAAGLRNVHSPREVMKESIEETGRAVVHD
                     LERFLTTLGTIASISPLMGLLGTIIGMIDIFASQGVSGANPAQLAQGISIALYTTGLG
                     LIIAIPAMIFWRHFRALIDSFVIDMEQQAIKLVEVSHGERRA"
     gene            12852..14225
                     /gene="xseA"
                     /locus_tag="PKN25_05825"
     CDS             12852..14225
                     /gene="xseA"
                     /locus_tag="PKN25_05825"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNV90454.1"
                     /translation="MPANPLLPANGAATPAAQVLSVSELNRMARELLESALPLMWVGG
                     EISNLVRAASGHVYFTLKDASAQVRCAMWRNRAQLLAFRPENGMRVEARALVTLYEAR
                     GDYQLSVEALRPAGIGSLFEAFNRLKAKLAAEGLFDEAGRRALPRYPRALGIVTSPQA
                     AALRDVLVTLRRRAPHLPVVLYPAPVQGADAPARLLEAVRCAGRRAAEDGVDVLLLVR
                     GGGSIEDLWAFNDEALARTLRACPLPVVCGVGHETDFTIADFAADLRAPTPSGAAELA
                     SAGWYAARAELAVLEPRLRRAVERRFGELAQRLDRAALRLVHPRERLRRERDTLARLG
                     ERLHHAAARRLEAADLRATRAGLRLRAAAPRPQALAARVDMLGGRLARAATRLLEARS
                     QRLDALAAHLQHLAPQAVLARGYAIARDKQGRVLRSAAGIPEGAAVSVQLADGRLDTR
                     VIGHGKA"
     gene            14362..14946
                     /locus_tag="PKN25_05830"
     CDS             14362..14946
                     /locus_tag="PKN25_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNV90455.1"
                     /translation="MEHTLPALPYAKDALAPHISAETLEFHYGKHHQAYVTNLNNLIK
                     GTEYENLDLEAIVKKAPAGGVYNNSAQVWNHTFFWNSMKPNGGGEPSGALADAIKAKW
                     GSFDDFKKAFQASAVGNFGSAWTWLVKKADGSVDIVNMGAAGTPLTTGDKALLCIDVW
                     EHAYYIDYRNRRPDFVATFLDKLANWDFAAKNFA"
     gene            15089..15646
                     /locus_tag="PKN25_05835"
                     /pseudo
     CDS             15089..15646
                     /locus_tag="PKN25_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            15959..17035
                     /gene="tal"
                     /locus_tag="PKN25_05840"
     CDS             15959..17035
                     /gene="tal"
                     /locus_tag="PKN25_05840"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNV90456.1"
                     /translation="MNPLLQVRQHGQQIWLDNLSRTLLEEGHLARFVADDGVAGVTTN
                     PAIFHKAISGGRYYEDDLAALKQQPLSAEARYEALVIPDVQRACDLLAPLHRDSGGSA
                     GYVSLEVSPALAHDADGTVAAGLRLKAAVDRPNLLIKVPATPAGLVAIERLIGEGVSV
                     NVTLMFSLAHCEGVAEAYLRGLARLRAAGGDVAGVMSVASLFLSRVDTLVDKLLEERG
                     GDLPALRGRTAVAMARLAYEAYQERFHGAGFEDLAAAGARPQYMLWASTGTKNPAYSD
                     LLYVEPLIGAETINTLPDATLDALRDHGRVASTLEQDVEQAAAHFTALAAAGIDLVAV
                     GERLQQEGLAQFEQAFAGLLELTA"
     gene            complement(17078..18967)
                     /locus_tag="PKN25_05845"
     CDS             complement(17078..18967)
                     /locus_tag="PKN25_05845"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNV90457.1"
                     /translation="MEDLLQDFLVEAGDLLSDVDNKLVELEHSPDDPGLLNDIFRGFH
                     TIKGGAGFLGAAELVTLCHLTESLFDRLRKHELEVTPERMDVILSATGAVRDMFVDLG
                     RGNRPGPAAPDLLEALRAACAGRPAAAAAGAPVAPAAEVRPAGVVLPRPGGPDWDALY
                     AAVTGATAPAGAPVAAPAIPRPAPLPALVPAAAAAAADPVVAAAFGRRASDVPGAGAP
                     VGRREGERQRDNSIRVDTARLDQVLNLSGEIGLTKNRLNALRSDILAGRTDTETLQAL
                     DLAVSQLDLLVSDLQNAVMKTRMQPIGRLFHKYPRIARDLARNLGKEVELALVGEDTE
                     IDKTMVEDLSDPIIHLIRNAVDHGIEDPARRAAAGKPAKSVLRLEARQEGDHIVITVA
                     DDGRGMDPERLRAKALAQGVITAEEASTLDERQSYDLIFLPGFSTAEKVSDVSGRGVG
                     MDVVRTNIQKLNGSIEIQSQLGKGTTLLIHLPLTLAILPVLLVRLGDQPFAVPLSMVR
                     EILPIEPGGIQDVGGKATMVVRGEVLPIVPLSALLGWPREAVPQYGVVMQSGAAVFIL
                     AIDSFAGREDAVIKSLEVFRPKGVAGVTTLANGQIVLILDMKELLESAGDRRSVSRST
                     LFEAA"
     gene            complement(19061..19915)
                     /gene="cheZ"
                     /locus_tag="PKN25_05850"
     CDS             complement(19061..19915)
                     /gene="cheZ"
                     /locus_tag="PKN25_05850"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HNV90458.1"
                     /translation="MSKKSKVDSMGDSDELQALFDSIAADPFAAAGAGAEPAASILPV
                     DESGDNDELQALFDAVAEQAGAAAPVAGGTVENAGAAAGEGVVYNRLGQLARQVHDAL
                     RELGLDTTLHRAAEAVPDARQRLTYVARTTEQAASRVLNATDVAGPIQTELANGADAL
                     CARWDELYAGRLSPEAFRELSADTRAFLGTVGDGARSTGAQLHEIMMAQDFQDLTGQV
                     IKRLVELTRTLETQLLQVLIEAAPAQVLEPEQAAGLLNGPVIDPAGRSDVVQDQAQVD
                     ELLERLGF"
     gene            complement(19947..20345)
                     /gene="cheY"
                     /locus_tag="PKN25_05855"
     CDS             complement(19947..20345)
                     /gene="cheY"
                     /locus_tag="PKN25_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HNV90459.1"
                     /translation="MSDPKTRFLVVDDFSTMRRIVRNLLKELGFANVDEAEDGAVALQ
                     KLKSSPYDFVVTDWNMPNMDGLTLLQSIRRTPELKHLPVLMITAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLAEKLEKIFEKMGRGRA"
     gene            complement(20376..21335)
                     /locus_tag="PKN25_05860"
     CDS             complement(20376..21335)
                     /locus_tag="PKN25_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNV90460.1"
                     /translation="MEHNRHEESALFDAVDGRATLAGSNRMEILLFSLGTGEHFGINV
                     FKVREVSRTPFITRTPNMPRGVEGLISLRGNVIPVLSLGVILGLTSAGAPLGEAMMVT
                     EYSKRTLGFLVDSVDRIVRVDWERVRAPENVSTGTQGFITAITELDDGRLVSILDVES
                     ILADTFGEAVVGDIAPIGNGHEVNVFFVDDSMVARRKITEVLDRLGVRHKHATNGMEA
                     WKRLDSLAAHAENTGHRLIDEIDLIMLDAEMPEMDGYVLTRRIKSDTRFAGIPVVMHS
                     SLSSEANRAMGTRVGVDAYVAKFDAEVLADTIRPMLQRARRTS"
     gene            complement(21345..22313)
                     /locus_tag="PKN25_05865"
     CDS             complement(21345..22313)
                     /locus_tag="PKN25_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNV90461.1"
                     /translation="MSELLKNIDARTRLAGTNKLEILLFTLGVDARSGCRETYGINVF
                     KVREVMRRPTITAAPDMPDAVQGMVSLRGALVPVVDLGRYVGVGDDAAREIMIVTEYN
                     GHTQGFLVEGVDTILRLDWSQMRVPPEMLTARLGGLVTAVTSLADGRLVMMLDVERVL
                     SETSRYDDAFLFRDIAPLPGADAHVVYFADDSSVARKQISQTLDAMGLRHVGAVNGRQ
                     AWEELSRVAARAEANGRKVADFVSLVLTDVEMPEMDGFMLTRQIKADRRFAGIPVIMH
                     SSLSGSSNHQLGLSVGVDEYVSKFEPQRLSQVLTRLLGMAEAASGR"
     gene            complement(22330..23322)
                     /gene="motB"
                     /locus_tag="PKN25_05870"
     CDS             complement(22330..23322)
                     /gene="motB"
                     /locus_tag="PKN25_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNV90462.1"
                     /translation="MSDDTQQPIVVKRIKKGGGGHHGGAWKIAYADFVTAMMAFFLLM
                     WLLGSTAQGDLEGIADYFQNPLKLAMQGGEGTGDSSSIIKGGGEDLSRKVGQVRRGDT
                     PSTRAINLDAAPARVKSEAEATAQAIAERRERARLIDLKGKLEAVIEANPSLRQFKNQ
                     LLIDIVSEGLRIQLVDAQNRPMFTSASAELRPYTRELLREIAAALNDVDNRVSMSGHT
                     DSAQYAGGERGFSNWELSSNRANAARRELVAGGLEAGKMLRVVGLADTAHLNPGDPLD
                     PANRRISIVVLNRAAEEAMRSQQAPGATPPPGGAQVGRNPADMRGSPGIVPAPN"
     gene            complement(23399..24253)
                     /gene="motA"
                     /locus_tag="PKN25_05875"
     CDS             complement(23399..24253)
                     /gene="motA"
                     /locus_tag="PKN25_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HNV90463.1"
                     /translation="MFLIIGYVIILAASLGTYAVHGSLAALWVPMEYLAIIGLMIGGF
                     VAGNGPKAIKGTLGSLSLAVKGSKYNKALYVDLLALLFEILAKVRKEGLMSIENDIEN
                     PDSSPIFSKYPSVAADHHAVEFMTDYLRMMVGGNLNAFEIENLMDMEIETHHQEAHQA
                     PHVVSKVADAVPAFGIVVAVMGVVNVMGSVGQPPAVLGKMIGGALVGTFLGILVSYGF
                     VAPIASQLEQKVEESGKIYQVIKVVLLASMNGYAPQVAVEFGRKVLYSTDRPGFAELE
                     QEIKNRKG"
     gene            complement(24446..>25025)
                     /gene="flhC"
                     /locus_tag="PKN25_05880"
     CDS             complement(24446..>25025)
                     /gene="flhC"
                     /locus_tag="PKN25_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HNV90464.1"
                     /translation="KSVIEEADDVRRAVEMVQLGARMQMLEVETRLSREKLLRIYKEV
                     RGASPPKGMLPFSTDWFMTWQPNVHSSLFMNLYRYFTGPAGVHGLDAILKSYHLYLDH
                     IETGVIEPALSLTRAWTLVRFFDADLLQMAACTRCGGEFVAHAHDPVHDYVCGLCNMP
                     SRAGSARRKTTTRKAVAPTHKTTAASRKAVVA"
BASE COUNT         3881 a         9002 c         8488 g         3654 t
ORIGIN      
        1 tgaacctgca caagaccttc tgcatcccgc acggcggcgg cggtccgggc atggggccga
       61 tcggcctggc ggcgcacctc gcgcccttca tggccgacca cgtgatccag cccaccggcg
      121 gcgccggccg gcccaacctg ggccagggcg cagtctcggc cgcgcccttc ggctcggcga
      181 gcatcctgcc gatctcgtgg atgtacatca cgatgatggg cggcgaaggc ctcaagcagg
      241 cgaccgaggt ggcgatcctc aacgccaact acctggccgc acgcctgaag gatcactacc
      301 cggtgctcta caccggcagc aagggccggg tcgcgcacga gtgcatcctc gacatccgtc
      361 cgatcaaggc gaccaccggc atctccgagg tcgacatcgc caagcgcctg atggactacg
      421 gcttccacgc gccgaccatg tcgttcccgg tggcaggcac gatcatggtc gagccgaccg
      481 agtcggaaga cctcggcgag ctcgagcgct tcgtcgccgc gatgatcgcg atccgcgacg
      541 agatccgcca gatcgaagcc ggcaactggc cggccgagga caacccgctc aagcacgcgc
      601 cgcacaccca ggccgacttc ctcggcgact ggaagcggcc ctactcgcgc gagcaggcgg
      661 tgttcccgtt gccctgggtg gccgagaaca agttctggcc cagcgtcaat cgcatcgacg
      721 acgtctatgg cgaccgcaac ctgttctgcg cctgcgtgcc gatgagcgac tacgccgagt
      781 gagcgccgcc cgcagcggcg aaccacgccc cgagcgctgg tagaatccgc cagcctcggc
      841 agcaagagcc acgcccgcgg gtgtggctct ttttttgccg ccagccctcc ccaccaccta
      901 cccccccgaa cctggaagga accggacacg gtgatcttcg ctccgcgcac caaccgcagc
      961 cagctctggc tcgccctgct catcgccagc accaccatgc tcctgctgct cgccgccatg
     1021 ctgtggcagc agacgcgccc ggtgcgcatg cacgacctgc acctcgccga gggcgagaag
     1081 ctgcgctgcg tgtcctacgc cccctatcat cgcccggggc agaccccgct cctcgaagac
     1141 acccgcatcg agcgcgagca gatcgccgcc gacctcgccg cgctggcgaa gatcaccgac
     1201 tgcgtgcgcc tgtactcgat cgaccagggc ctcgagcacg tccccgagct cgcccgtccg
     1261 ctcggtctga aagtgctgct cggcgcctgg atcggctacg agaagacgaa gaacgcccgc
     1321 gaactcgagc gcgcgatcgc gctcgccaac gccaacgccg acgtcgtgcg tgcgctcatc
     1381 gtcggcaacg aggtcctgct gcgccgcgag cgcaccgagg acgagatgcg cgaactcatc
     1441 cgtcacgcca aggcgcatgc gaaggtgccg gtcacctacg ccgacgtgtg ggagttctgg
     1501 acccgccacg acagtctcgc caacgaggtc gacttcgtca ccgtgcacat cctgcccttc
     1561 tgggaggacg aaccggtcga catcgagctc gcgctccagc acgtggccga aacgcggcgc
     1621 cacgtcggtg agcacttcgc cggcaagaag atcctcatcg gcgagaccgg ctggccgagc
     1681 gcgggccgcc agcgcgagga atcgaagccc tcccgggtca accaggcgcg ctacatccgc
     1741 gagttcgtcc atcgcgcgca tgccgaaggc tgggactaca acctcatcga ggccatcgac
     1801 cagccctgga agcgccgcct cgaaggcacg gtgggcggct tctggggcat gctcgacgcc
     1861 gacagcctgg agcccaagtt cccgctcgcc ggtgcggtgg ccgagcgcga aggcgtggcc
     1921 ggtccggtcg gcggcgctgc ggtcggcggc gtgctcgcct tcctgctcgc gaccaccggc
     1981 cgacgcacgc gctgcctgcg cgcaagcgcg atcgccgccg caggcacact cggcggaatg
     2041 atcacggtgc tgcactggga acacgcccac ctggcctacc gcgatgcgct ggagtggagc
     2101 ctgctcggcg gtgtcgccgc gctcggcgcc ctgctgccgc tcacgctcgc gcgctggcat
     2161 ggcgagcccg tgcccgccac cggccgcgcc gcgcgcatcc tgcggcatgg cgaacccctg
     2221 cgcgccaagg ccggcgcgct cggcctgctg cgcggcctgc tgctcttcgc agccgcggtg
     2281 gccgcgctgc tgctgtgggt cgatccgcgc taccgcgact tccccaccct gctctacctg
     2341 gtgccggcgg tggtgctcgg tgtcgtcggc tggtggcgcg ccgacaccac gcgcagcgag
     2401 atcaccctcg cgctggtcat cctcatcggc gtggtcgcgc gctggtcaag cgagccggcc
     2461 aacccccagg cggtcgcctg gctgctcacc gggctcgcgc tggccctgcc cgtgctgctg
     2521 gtgcgctcgc accagcacga gcagcgcgag tagggcgccg accgcggccg ggtcgtagct
     2581 gtacagcacc aggccggcca ctccacacag ccagcccgcc caggccaggc cgcgactggc
     2641 acgcacaaag gcgagcacgc ccgcgacgag gctcacccag ccgatgcgct ggtcgaggaa
     2701 gctgtgcacg aaggcctgct tgaacaggca ggccagcgcg ggcgcatcgg cggccgtgca
     2761 gtcgcgcggc agcaccccgg cctcgagcac gccgtggcgc aaccagagcg cgagtgcggc
     2821 gagcgcgagt ccggcgagga gcggacgggc gacgccatat ttgagcaaat tagttggcat
     2881 tgggatacgc tttcccttca aaatcagcac acgccgacaa gacgtgcaaa gtgttgcaaa
     2941 ttgccacccc gagcggctcg taaggggctg aaatggcgaa taaaatgcgc gccgtcggtg
     3001 tgaagggcgg acgccctgcc gggcgcgcgc attttgtcac agtgcggccg ggtcggcgat
     3061 cgtggcggcg cagcgccttc atggcctgcc cgccgcagcc gcccggtgtc accccgacac
     3121 gcggcccacg aggccctcgc aatacatccg cgccgtacct gcaggacggc gcccaggaac
     3181 cacgatgaag aacgccgcct ccttcgcaat ccgcatcctc accgcaatcg ccatcgccgc
     3241 ggtggtcgcc ttcgcgcaat actggatctg gcagcagctc aaccgcagca ccgagttcat
     3301 cggcaccaac cagagcatca agggcttcgc ctacaacggc ttccagcgcg accagagccc
     3361 gctcaagggg acctacccca cgcgcgccga actcgcctcc gacctcgacc tgctcggacg
     3421 ctactccgac ggcctgcgca cctacggcgt caacgacatg cccgagctgc tcgatctcgc
     3481 cggcgagcgc gacatgctgg tcacggccgg cgcctggatc gacgcccgcc ccgagtccaa
     3541 cgcgcgcgaa gtcgcagccc tgatcgaggc ggcacgcaag atgcgccaca tcgagcgcgt
     3601 catggtcggc aacgaggcca tcctgcgcgg cgacgtcacg gtcgacgagc tcatcgtcta
     3661 tctcgacgag gtgcgcaagg cgatccgcaa gccggtctcg accgccgagc cctggcacgt
     3721 ctggctgcgc tatccggaac tcgccaagca cgtcgactac atcaccgtgc acctcctgcc
     3781 gtaccacgag ggcctgccgg tggacaaggc ggtggagtac gccttccagc gctacgacga
     3841 ggtcgcgcgc gcacacccgc gcaagaagat cgtggtcggc gaagtgggct ggccgagccg
     3901 cggcccgacc atcgacgccg ccatcccctc gctcgacaac caggcgcgct tcgtgcgcga
     3961 gttcctcgcc catccgcgca ccgcacgcat cgactacttc ctgatggaag cgatcgacca
     4021 gccgtggaag gtggacgtcg aaggctgggc cgggccctac tggggcatgt tcaacgccga
     4081 ccgcgagccc aagtaccagc tcgagggcgt ggtcgaacgc gacccgcact ggtcgcacaa
     4141 ggccagcaac gccgccgcac tcgccttcat cccgatgatg ctggcggcct tcttcctgcc
     4201 cggctggagc atcggcggcc gcctcttcct cgccgcgctg atccaggcct gcatctcgac
     4261 gctgatcatc ggcatcaacg tgccggtcga gtactacctc acccagcgcg acctcatcgg
     4321 tctggtgctg ctgatcggtg ccacctgcat gaccgcggcg gtgctgctgt cgcacggctt
     4381 cgagttcggc gaggtgctct tcaagaagaa atgggcgcgc cgcttcaccc cgctgccgcc
     4441 gcacccgccc gagcagcagc ccttcgtgtc catccacctg gcctgctaca acgagccgcc
     4501 cgagatggtg atcgccacca tcgacagcct ggcgcagatg aattaccaga acttcgaggt
     4561 cctgatcctc gacaacaaca cccgcgacga ggccctgtgg aagccgctcg agcgccgctg
     4621 cgccgagctc ggtccgcgct tccgcttctt ccacctcgcc aactggcccg gcttcaaggc
     4681 cggcgcgctc aactacggcc tcaaggtcac cgacccgcgc gccgaggtgg tgggtgtcgt
     4741 cgacgccgac tacgtggtcg atcccgactg gctggcctgc ctggtgccgc acttcgacca
     4801 gcccgaggtc gccgtcgtgc aggccccgca ggcccaccgc gactgggagg gccagccctt
     4861 caagcgcatg tgcaactggg agttcgacgg cttcttccgc atcggcatgc accaccgcaa
     4921 cgagcgcaac gcgctgatcc agcacggcac catgaccatg gtgcgccggc gcgcactcga
     4981 ggaggtcggc ggctggtccg agtggtgcat ctgcgaggac accgagctcg gcctgcgcct
     5041 gatcgagaag ggctacgaca cgcgttacat cgaccatatc ctcggccgcg gcctcacccc
     5101 ctcgggcttc gccgcgatca agagccagcg cttccgctgg gccttcggcg cgatgcagat
     5161 cctcaaggcc cacctgcccc acatgatcgg gcgcagcacc ctcaacctcg cccagcgcta
     5221 ccacttcctc accggctggt tcgcctggct gggcgacgcg ctgcagctgg tgttcgcctt
     5281 cggcagcctg ctgtggaccc tgggcatcct gctcttcccc aaggccttcg gcctgccggt
     5341 ggtgtcgctc gcgctgccga tcttcggctt catgatcttc aaggcagcgc tcggccccat
     5401 cctctatcgg cgcacgatgg actgcccgtg gaaggacatc ctcggcgcct caatcctgtc
     5461 ggtgggcctc gcccacgcga tcgcgcgcgg cgtgttcgcc ggcctggtga aaaagaaagg
     5521 cgtgttcgtg gtgacgccca agggctggcg cggcggtggc gcgctggcct tcttcaaccc
     5581 catccgcgag gagatcggca tgctggtcgc gctgctgatg ggcgccgtca ccctggtcgc
     5641 gcagcgcggc gccaacaacc tcgagaccca gctgtgggtc ggcatcctgt gcctgcagtg
     5701 catcccctac gtggccgcga tcctgtgcca ggtggcggcc tacatgccgg agcgtggggc
     5761 cgcggccggg gaaacggccg cggcagcgtg aggagtcggg cgcccggtgt cgggtgcaaa
     5821 gtcgaaacct cccgccgctc gatcctcgcc gggcggcggg aaggtcggac gccgtccggc
     5881 cttctccgct ccagacgcct cactcctcac gccaccgcaa tcaacgccgc cagcaccagc
     5941 gccggataga ccagcaccgt caccccggtt ccgcccagga tcatcacccg ctgcggcacg
     6001 tggccggtgc cgaagacgat cgcgttgggc ggagtggcaa cggggagcat gaaggcacac
     6061 gaggtgccga ccgcgaccag cacggcgagc ggcgcgcgct cgacatgcgg ggcgagaccg
     6121 aggaagagcg ggatcagcag ggctgcactc gcggtgttgc tggtgacctc ggtgagcagc
     6181 aacgcgaaca gcaccatcag cgcgatgcag ccccaggccg gcagtgcggc gagcggtcca
     6241 ccgagctggg tggcgagcca ggccgccgcg ccgctggccg cgagcgcctt gctcagcgtg
     6301 agcccgccgc cgaagagcaa cagcacgccc cactcggtgc tgcgctccac gtcctgccag
     6361 cccgccagcc gcaagccgtg cagcagcacg agcgcgaaca gcgccaccgc ggcgtcgtag
     6421 ccctgggcaa agccgagcgc ggcgccgagg ggcgcgccga acacccacag cagcacggtg
     6481 aagccgaaga gtgcgagcat cttgcgctgc gccggagtcc atcctccggg aggcggtggc
     6541 ggcgtcaccg ccacgtcgag ccgcggtcgc agcaccaggc gcagaccgat ctccatcagc
     6601 ggcatcatca ccagcaccag cggcagaccc cagctcatcc attcggcgaa atccatgccc
     6661 accgctgccg cggcgatcgc gttgggcggg ctgccgacca gggtgccgat gccgccgatg
     6721 ttggcggaga aggccacgcc gagcagcacg tacatccagg tgcggcgatg ttcgtcgagc
     6781 ggcagcgggg cgagcaggcc aagcgccagc ggcagcatca tcgccgcggt cgcggtgttg
     6841 ctgatccaca tcgacaggaa ggcggtcaac cagaacagcc agcgcaccgc gcgcccgaag
     6901 ctggcccccg cgcggcgcag gacctggccc gccatccagc ggtccagccc ctggcgctgc
     6961 aggccggcgg cgagcgcgaa gccgccgagg aagaggaaga ctaccgggtc ggcgaactgc
     7021 agcaaggcct cgggaaaatc cagcactccg ctcgcggcgg cgagcagggg caccagcagc
     7081 gcggtcacgc tcacgtgcag ggcttcggtc agccacagcc aggcgatcgt ggcgagcaga
     7141 gcaagcccgg ctccggcgcg gtccggcaga ccctccaagc cccagagcag gccgaggaag
     7201 agtacgcccg cgacgactag attgaggttc cgcatcaatt gatccacccc tgcgcagtgg
     7261 cgctatcggc tcgagcacac acccgtacgt acgcggcgtt gcacgccgat ccgcgtcagg
     7321 cctgccacgc cgccagccgg cgcgccaccg gggcccagtc ccagcgcccg tagtcgggca
     7381 ggccgcccgc caccggcggc ggaatctcgc cctcgatcag cggacgcatg tagtcgcagg
     7441 ccgccgtggt cacgtgcagc ccgtcggcgc gcacgaaacc gtccggcagg ctgcgctcga
     7501 ggttggccac cgcggcggtg tccaccgcga ccacatccca gcggtagggc gcatcctgca
     7561 ggcggcggat cgcgggcatg gtcccgccct ggccggcgag cgcgtactcc accgccaccc
     7621 ttccgaccgc ttcggcctgc gcgcggtcgg tcgccgacag ccagtgtccg gccgagcgct
     7681 gcaggtagtc ggggatcgcc cagtggaact tgtagccgag gcgctcgttg atccgccgcg
     7741 ccactgccag acccgcgccg ccgagctgga cgtagccgcg cgcatcgcgc gacttctcca
     7801 tcagcaggct gccatccggt cggcggatgc cctccgccac cgtcaccgca caatagccca
     7861 gccgttcgac gatctcgcgc acgcgggcga ggaaggccgc ttcgtcgaag cgcgcctccg
     7921 gtaccagcac gatatgcggg gcgcggtccg ggtcgccctg cgcggcgagc gcggtggccg
     7981 cggccagcca gccggtgttg cgccccatca cctccatgac gaagacgctg cccttgctgc
     8041 ccaccatcga cgcggtgtcc agccccgcct ccagcatcga agtggcgagg tagcgcgcgg
     8101 ccgagccgaa gccgggacag cagtcggtac cgacgatgtc gttgtccacc gtcttgggca
     8161 cgcccacgca caccagcgca tggccgcgcc gcgcggcctc ggcctggatg cgcgcgaccg
     8221 cgtccatcga gccgttgccg ccgttgtaga gcagccagcc gatgtcgtgc gcgtcgaaaa
     8281 ccgcgaacag ccgatccatc gccgcgccgc cgtcgtcgtg cggcagatcg aagcggcagg
     8341 agccgaaggc cccgcccggg gtgcgcgcca ggcggacgag cgccgcctcg tccaggccgg
     8401 cggtatcgat cagggcctcc tcgagcacgc cgacgatgcc gcgccacgcg gcgaacacct
     8461 tgccgacccg acccgaggcg cgcgcggccg cgacgaccgc cgcggcgctg gcgttgatga
     8521 cggcggttac gccaccggaa tgggcataca gaaggttctg ctggcgcatc ggccgggcct
     8581 cgttcgagcg ttgccgcgcg ccgcgccggg cggagattgc ggcgagttca aaagcacgcg
     8641 cggccgtcgc cccgtggggg gcgcggccgc gccggatgag gccgggcatg cccggccggg
     8701 gtgctgccta cttcagcgct gcaaaggcgt tcgcggtgat cgcatccacc gcgccgaggc
     8761 cggagatctt gcgcaccttg ggcgccttgg cgtcgcccga ggccgcccac ttgctgtagt
     8821 actcgaccag cggcttggtc tgcgagtggt agacgtcgag gcgcttcttg acggtttctt
     8881 ccttgtcgtc gtcgcgctgc accaggtctt cgccggtgac gtcgtccttg cccgcgacct
     8941 tgggcgggtt gtacttgacg tggtaggtgc ggcccgaagc cagatgggcg cggcgcccgc
     9001 tcatgcgttc gacgatttcg gcgtccggca cgtcgatctc gagcacgaag tcgatcggca
     9061 cgccggcgtc cttcatcgcg tcggcctgcg ggatggtgcg cgggaagccg tcgaacatgt
     9121 agccggactt gcagtcttcc tgctgcaggc ggtccttcac caggccgatg atgatgtcgt
     9181 cggacacgag gccgccggca tccatgacct tcttcgcctc caggcccagc ggggtgcccg
     9241 ccttcaccgc ggcgcgcagc atgtcgccgg tggagatttg cggaatgccg aacttctcct
     9301 tgatgaagtt ggcttgcgta cccttgccgg ctcccggcgg tcccaacaga atcagacgca
     9361 tactccctcc cgtgaatggc cggccatcga aaatggccgt ttccttatcg aataagcagc
     9421 cgaaacttaa ccgacgcgcg ccgggtggtc aaacccctcc ggcggcgaag atcgcgcgca
     9481 cccgctccag gtcctcctgc gtgtcgaccc cggccgccgg tgcctgggcg agttcgagca
     9541 cgcggatgcg atagccgtgc cagagcgcgc gcaactgctc gagcgcctcc cagtgctcca
     9601 gcggcgaggg cgcgagcgcc gcatagcggc gcaggaagcc gacgcggtag gcgtacaggc
     9661 cgacatggcg cagcaccggc agccctgcgg gcaggtcgcg cgcaccgtca ccccaggcgt
     9721 cgcgggccca ggggatgggc gcgcgcgaga agtacagcgc gcgcccggcg gcatcgcaca
     9781 ccaccttgac cacgttgggg ttgaagacct cgacgggatc gtggatggcg tgcgcggcgg
     9841 tggcgatggc agcctcgccg tcggcggcga gcgcgcgcgc caccgcagcg acgatctccg
     9901 ggtcgatcag cggctcgtcg ccctgcacat tcaccacgat atcctcgtcg gcccagccgc
     9961 gcgcgagcgc gacctcggcg aggcggtcgg tgccgctcgg atggtccggg cgcgtcatca
    10021 ccacggcacc gcccgcagcc tgcacgacct cgcgcacgcc ctcgtggtcg gtggcgaccc
    10081 aagtctcgct ggcgccggca tgccgcacgc gctcgagcac acgcacgatc atcggcttgc
    10141 cgccgatgtc ggcgagcggc ttggcgggca gccgggtcga ggcgtagcgc gcagggacga
    10201 cgatgcggaa gtcggtggac atgccgccgc tcatttgcgc agggcgtcga cttcctcggc
    10261 gctcatggcg cgcgcctcct cgtccagcat caccgggatg ccgtcgcgga tcggataggc
    10321 aaggcggtcg gccttgcaca ccagttcctg cttgtccttc agatagtcga gcggcccctt
    10381 gcacagcggg cagacgagga tttcaagcag acgtgcgtcc atcttcgagt ttctccagga
    10441 tgcgttcggc ggcgccggaa ccgatctgcg cccgcaccgg gtattcccag cagtcggcag
    10501 gcgcgaaagc ccggcatttt accgcatcct tcgcggtcat caggatcggc gacgcgtcgc
    10561 cgaagacgag atcggcggcg acgaagcgat gatgatcggg gaaggcatgg gcgaccacct
    10621 ccaggccggc ggcggcgagc tggtcgaaga agcgctgcgg cctgccgatc ccggccaccg
    10681 cgtgcacccg gcgaccacgc agcgccgcga gcgggaggcg acacgcaggg tcggcgagcg
    10741 cgaccacctc ttcgccgaag agccgcatcg gaaacaccgg cacgccgccg atcgccgcgc
    10801 gcagctcggc atccagctcg ccgtgggcca gcaccagatc gacttcggcg aggcgcgcga
    10861 gcggctcgcg cagcggcccg gcgggcagca gcaggcggtt gcccagcgtg gccgcgtcca
    10921 ccacggcgat ctccaggtct cttgcgagat ggtagtgctg catgccgtcg tcggcgatga
    10981 gcacgtcgca ctcgggatgc aggcgcagca gcgcgcgcgc agcggcggga cggtcctgcc
    11041 ccaccaccag cgggcaagcg gtaagacggg cgagcagcac cggctcgtcg ccatagcggc
    11101 cggggtcgcc gtctgcgggc acaagggcga gcccgccctg cgcactcgcc gtaccgccat
    11161 ggccgcggct gacgatgccg gggtggtggc cagcgacacg cagcaccccg gccagccagt
    11221 cgaccaccgg cgtcttgcca ctgccgccga ccgcgacgtt gccgaccacg atcaccggca
    11281 ccggcaggcg ctgcggctgc gtgagcgcgc ggcggcggcg ggcgaggaaa ccgaacagca
    11341 gggagagcgg acgcaaggcc tgcgcgagca ggccgcgacg ctgccagaac gctggggcct
    11401 gcgcagccac gttcagtctc cggggcggcg cgggccggag gacctcactg cgccgacgac
    11461 tgggtggcga aggtgatgtg cggcagcccc gcgcgctgcg cggcctgcat cacgtccacc
    11521 acgctctggt gcggggtgcg cgcgtcggcg ttgatcacca ccaccggcgg cgtgtcgccg
    11581 tcggccgggg ccgacttgcc gagcgcggcg gcgatcgcct cgacgctgcg ctcacccact
    11641 gcctggcgat tcaccaggac ctcgccggcg gcggttaccg cgaccacgat ctcgcgcgcc
    11701 tcggtctcgc tgccctcggc ctcggcggtg ggcagcttga tctccagccc ggagaccttg
    11761 gagaacgtgg tggtcagcat caggaagatg atgatcacca acaccacgtc gatgagcggg
    11821 atcaggttga tctcgggctc gccgttgcgg ctgccgcgac ggaagttcat cgcccctccc
    11881 ttacgcccgg cgctcgccgt gggagacctc gacgagcttg atcgcctgct gctccatgtc
    11941 gatcacgaag ctgtcgatca gcgcgcggaa gtggcgccag aaaatcatcg ccggaatggc
    12001 gatgatgagg cccagcccgg tcgtatacag cgcgatcgag atgccctggg cgagctgggc
    12061 cgggttggcg ccgctcacgc cctgcgaggc gaagatgtcg atcatgccga tgatggtgcc
    12121 gagcaggccc atcagcgggc tgatggaggc gatggtgccg agcgtggtga ggaagcgctc
    12181 gaggtcgtgc accaccgcgc gcccggtctc ctcgatcgac tccttcatca cctcgcgcgg
    12241 gctgtgcacg ttgcgcaggc cggcggcgag cacgcggccg agcggcgagt gcgccgcgac
    12301 gcgctcggcc atctcggccg agggcccgat ctggcgcaga tcgtcgagga ccttgtcgag
    12361 cagccccggt ggcacgatgc gcgaacggcg cagggtgatc gagcgctcga tgatgagcgc
    12421 caccgcgatg accgacgcca gaatcagggg ccagatcggc cagccaacgg cctggatgat
    12481 cgcgaacacg cgggctcacc tctcgattgg aaaggcgaga ctctagcgcc gcgccccccg
    12541 cccggcaagc cgcatgcatc acgctgcgca cgcgggcacc gggcatccgc gggcacgggc
    12601 atggcctgcg cacgaggaga tcgggagcgc acgcggcaat ccacagaagc tgtggataag
    12661 tttgtggacg atttcggaat cctgcccgca aagcccgcaa cggcaggctt tccctcactc
    12721 cgatgcaaaa ttgtgcaagc catcgaacct ttacgaatca ataacttgca gaatcacccc
    12781 ttatcgatca aggacttgca aacacaccct tgacaaggtc gaaagcctgt ggattccgct
    12841 acaatccggc catgcccgcg aaccccctct tgcccgcaaa cggcgccgcg acgcccgccg
    12901 cgcaggtcct cagcgtctcc gaactcaacc gtatggcgcg cgagctgctc gaatccgcgc
    12961 tgcccctgat gtgggtgggc ggcgagatct ccaacctcgt ccgcgccgcc tccggccatg
    13021 tctacttcac cctcaaggac gcctccgccc aggtgcgctg tgcgatgtgg cgcaaccgcg
    13081 cgcagctgct cgccttccgc cccgagaatg gcatgcgcgt cgaggcgcgc gcgctcgtca
    13141 ccctgtacga ggcgcgcggc gactaccaac tgagcgtcga ggcgctgcgc ccggccggta
    13201 tcggcagcct gttcgaggcc ttcaaccggc tcaaggccaa gctcgccgcc gaaggcctct
    13261 tcgacgaggc cggccggcgc gcgctgccgc gctacccgcg cgcgctcggt atcgtcacct
    13321 cgccgcaggc cgccgcgctg cgcgacgtgc tggtgacgct gcgccggcgc gcgccgcatc
    13381 tgccggtggt gctctacccc gcgccggtgc agggcgccga cgctcccgcg cgcctgctcg
    13441 aggcggtccg ctgcgccggc cggcgcgccg ccgaagatgg cgtcgatgtg ctgctgctgg
    13501 tgcgtggcgg cggcagcatc gaggacctgt gggcattcaa cgacgaagcc ctggcgcgta
    13561 ccctgcgcgc ctgcccgctg ccggtggtgt gcggcgtggg ccacgagacc gacttcacca
    13621 tcgccgactt cgccgccgac ctgcgcgcac ccacgccgag cggcgccgcc gagctcgcca
    13681 gcgccggctg gtacgctgcg cgcgccgaac tcgcagtgct cgaaccgcgc ctgcgccgcg
    13741 ccgtcgagcg ccgcttcggc gagctcgcgc agcgcctgga ccgcgccgcg ctgcgcctgg
    13801 tgcatccgcg cgaacgcctg cgtcgcgagc gcgacacgct ggcgcgcctg ggcgagcgcc
    13861 tgcaccacgc cgccgcgcgt cggctcgagg ccgccgacct gcgcgccacc cgcgccgggc
    13921 ttcgcctgcg tgccgcagcg ccgcgtccgc aagcgctcgc cgcgcgcgtc gacatgctcg
    13981 gtgggcgcct cgcgcgcgca gccacccgcc tgctggaagc ccgcagccag cgcctcgacg
    14041 ccctcgccgc ccacctccag catctcgcgc cacaggcggt actggcgcgc ggctatgcca
    14101 tcgcccgtga caagcaaggc cgcgtcctgc gcagcgccgc cggcatcccc gagggcgccg
    14161 cggtgagcgt gcagctcgcg gacggccgcc tcgacacccg ggtcatcggc cacgggaagg
    14221 cctgagcgca cgggttccgc aaatcccccg catgggctta ccatgcggga agcaagcttg
    14281 ttcggggtct aaatccagac taaaatagtc cggcttctac ccccaacccg aacacagaca
    14341 gaccaacaac ggagatcacg catggaacac accctgcccg ccctgcccta tgccaaggac
    14401 gccctcgccc cgcacatctc ggccgagacc ctggagttcc actacggcaa gcaccaccag
    14461 gcctacgtca ccaacctgaa caacctgatc aagggcaccg agtacgagaa cctcgacctc
    14521 gaagccatcg tcaagaaggc ccccgccggc ggcgtgtata acaactccgc gcaggtgtgg
    14581 aaccacacct tcttctggaa cagcatgaag cccaacggcg gcggcgagcc cagcggcgcg
    14641 ctggccgacg cgatcaaggc caagtggggc tccttcgacg acttcaagaa ggccttccag
    14701 gcctccgcag tcggcaactt cggttccgcc tggacctggc tggtcaagaa ggccgacggc
    14761 tcggtcgaca tcgtcaacat gggcgccgcc ggcacccccc tgaccaccgg cgacaaggcc
    14821 ctgctctgta tcgacgtgtg ggaacacgcc tactacatcg actaccgcaa ccgtcgtccg
    14881 gacttcgtcg ccaccttcct cgacaagctg gccaactggg acttcgcggc gaagaacttc
    14941 gcctgattcc cggagccggc ccgcggccgg ccgagctgga tgcgaaagcg acccgcgtgg
    15001 tcgctttcgt gctttatggc cggtacgagc gtcggcgcgc gcgcgcagcc ccctgcgcgt
    15061 cggcgaggcg ccaccgacga gacacgagat gctcgagatc ctctaccgcg acgactggct
    15121 ggttgcgatc cacaagccct ccggcctgct cgtgcaccgc agtccgatcg ccgcgcacga
    15181 ggaacgcttc gcggtgcagc tgctgcgcga ccagctcggc cgccgcgtgt atcccgccca
    15241 ccggctggac cgcggcacct cgggcgtgct gctgttcgcg ctcgatcgcg aggtggcgcg
    15301 cacgctcgcg cagcgtttcg aggcgcaggc ggtcgacaag cgctacctgg cggtcgtgcg
    15361 cgggcatccg cccgagcacg gcgtgatcga gcacgcgctg gtacgccgac tcgatgcggt
    15421 cgaagtgcgc agcggcaagg gcgccggggc ccgcgacgcg ctgcccgagg atgtcgacga
    15481 cagtgattcg ccagaggctg ccgagccggt cgcccagccc gcgcgcaccc gcttccgccg
    15541 cctcgccacg gtcgagctgc cgcatgcggt ggaccgctac cccagcagcc gctacgcgct
    15601 cgtcgagctc ttccccgaga ccggccgccg ccatcagctg cgccgccacc tcaagcacat
    15661 cgcccacccg atcatcggcg acgccaccta cggcaagggc cgccataacc gcctgttcca
    15721 ggcgctcttc ggcagccacc gcctgctgct cgcgtgcacg cggctggcgc tggcgcatcc
    15781 ggtgacggag cgcccgctgg agatcgtggc gccggtggcg gaggattttg ccgcggtgct
    15841 cgcggcgctc ggctggcaga cggcacagcg cagcactttc gaggcagaat ccaggctttc
    15901 cgccgcccgc gggcccgcgc ccgagcggcc ccgttcccac gacactccag gtagaacgat
    15961 gaacccgctg ctccaggtac gccagcacgg ccagcagatc tggcttgaca acctctcccg
    16021 caccctgctc gaggaaggcc acctcgcccg cttcgtcgcc gacgacggcg tcgccggcgt
    16081 gaccaccaac ccggcgatct tccacaaggc gatttccggc gggcgttatt acgaggacga
    16141 tctcgccgcg ctcaagcagc agccgctgag cgccgaggcg cgctacgagg ccctggtgat
    16201 ccccgacgtg cagcgcgcct gcgacctgct cgcacccctg catcgggaca gcggcggcag
    16261 cgccggctac gtcagcctgg aagtctcgcc cgcgctcgcc cacgacgccg acggtaccgt
    16321 ggccgccggc ctgcgcctga aggccgcggt cgaccgcccc aacctgctga tcaaggtgcc
    16381 cgccacgccg gcgggcctgg tcgccatcga gcgcctgatc ggcgagggcg tcagcgtcaa
    16441 cgtcacgctg atgttctcgc tcgcgcactg cgagggggtg gccgaggcct acctgcgcgg
    16501 gctcgcgcgt ctgcgcgcgg cgggcggaga cgtcgcgggc gtgatgtcgg tggcgagcct
    16561 gttcctgtcg cgggtcgaca ccctggtgga caagctgctg gaagagcgcg gcggcgacct
    16621 gccggcgctg cgcggccgca ccgcggtggc gatggcacgg ctggcctacg aggcctacca
    16681 ggagcgcttc cacggcgccg gcttcgagga cctggccgcg gcgggcgcac gtccgcagta
    16741 catgttgtgg gcaagcaccg gcaccaagaa cccggcctac agcgacctgc tctacgtcga
    16801 gccgctgatc ggtgccgaga ccatcaacac cctgcccgac gccacgctcg acgccctgcg
    16861 cgaccacggc cgcgtggcct cgacgctgga acaggatgtc gagcaggccg ccgcccactt
    16921 caccgcgctc gcggccgccg gcatcgacct cgtggccgtg ggcgagcgcc tgcagcagga
    16981 aggcctggcg cagttcgagc aggccttcgc agggctgctc gaactcaccg cctgagttgc
    17041 cacctcgctc gccagccgag cacgaccggg gcggcggcta cgccgcctcg aacagcgtcg
    17101 agcgcgacac gctgcgccga tcgccggccg actcgagcag ctccttcatg tcgaggatca
    17161 gcacgatctg gccgttggcg agcgtggtca ccccggccac gcccttcggt cggaagacct
    17221 cgagcgactt gatcaccgcg tcctcgcgcc cggcgaagct gtcgatcgcg agaatgaaca
    17281 ccgcggcgcc cgactgcatc accacgccat actgcggcac cgcctcgcgc ggccagccga
    17341 gcagcgcgga cagcggcacg atcggcagca cctcgccgcg caccaccatg gtggccttgc
    17401 caccgacgtc ctggatgccg cccggctcga tcggcaggat ctcgcgcacc atcgacagcg
    17461 gcacggcgaa gggctgatcg ccgaggcgca ccagcagcac cggcaggatc gccagggtca
    17521 gcggcaggtg gatcagcagc gtggtgccct tgccgagctg ggactggatc tcgatgctgc
    17581 cgttgagttt ctggatgttg gtgcgcacca cgtccatgcc gacgccgcgg ccggacacgt
    17641 ccgacacctt ctcggcggtg gaaaacccgg gcaggaagat gaggtcgtag ctctggcgct
    17701 catcgagcgt gctcgcctcc tcggcggtga tcacgccttg cgcgagcgcc ttcgcgcgca
    17761 gcctctcggg atccatgccg cggccgtcgt cggccacggt gatcacgatg tggtcgccct
    17821 cctggcgcgc ctccaggcgc agcaccgact tggccggctt gccggcggcg gcgcgccggg
    17881 cgggatcctc gatgccgtga tccaccgcgt tgcggatgag gtggatgatc gggtcggaga
    17941 gatcctcgac catggtcttg tcgatctcgg tgtcctcgcc gaccaacgcc agctcgacct
    18001 ccttgccgag gttgcgcgcg agatcgcggg cgatgcgcgg gtacttgtgg aagagccggc
    18061 cgatcggctg catgcgcgtc ttcatcaccg cgttctgcag atcggagacg agcaggtcga
    18121 gctggctcac cgccaggtcg agcgcctgca gggtctcggt gtcggtgcgg ccggcaagga
    18181 tgtcgctgcg cagcgcgttg agccggttct tggtgaggcc gatctcgccc gagaggttga
    18241 gcacctgatc gaggcgggcg gtgtccaccc ggatcgagtt gtcgcgctga cgctcgccct
    18301 cgcgccgacc gaccggcgcg ccggcaccgg gcacgtcgct ggcgcggcgg ccgaaggcgg
    18361 cggcgaccac cgggtcggca gccgccgcgg cggcggcggg cacgagcgcg ggaagcggcg
    18421 cgggccgcgg gatcgcgggc gccgcgacgg gcgctccggc cggcgcggtc gcgccggtga
    18481 ccgcagcata caaggcatcc cagtcgggcc cgcccggccg cggcaggacc accccggcgg
    18541 gccgcacctc ggcggcgggc gcgacgggtg cccccgcggc ggctgccgcc ggtcgcccgg
    18601 cgcacgcggc gcgcagcgcc tcgagcaggt cgggcgcagc cggccccgga cggttgccgc
    18661 ggccgagatc gacaaacatg tcgcgcaccg cgccggtcgc cgacaggatc acgtccatgc
    18721 gctcgggggt gacctcgagc tcgtgcttgc gcaggcgatc gaataggctc tcggtgaggt
    18781 ggcacagggt caccagctcg gccgcgccga ggaagcccgc gccgcccttg atggtgtgga
    18841 agccacggaa gatgtcgttg agcaggccgg ggtcatccgg gctgtgctcg agttcgacga
    18901 gcttgttgtc gacgtcggac agcaggtcgc cggcttcgac gaggaagtcc tgcagcagat
    18961 cctccatccc ggcaaaatca ctcacggctt gctcctggtt gcggtgcgac cccacgcggg
    19021 acgatcaccg gttcgcgcgg ggtccggccc cgcggcttgg tcagaatccg agacgctcga
    19081 gcagctcgtc gacctgggcc tggtcctgca ccacgtcgct gcgcccggcc gggtcgatca
    19141 ccggtccgtt gagcagcccg gccgcctgct cgggctcgag cacctgcgcc ggcgcggcct
    19201 cgatcagcac ctgcagcagc tgggtctcga gcgtgcgggt gagctcgacc aggcgcttga
    19261 tcacctgtcc ggtgaggtcc tggaagtcct gcgccatcat gatctcgtgc agctgcgcgc
    19321 cggtgctgcg cgcgccgtcg cccaccgtgc cgaggaaggc gcgcgtgtcg gcggagagtt
    19381 cgcggaaggc ctcgggactc aggcggccgg cgtagagttc gtcccagcgc gcgcacagcg
    19441 catcggcccc gttcgcgagt tccgtctgga tcggccctgc cacgtcggtg gcgttgagca
    19501 cccggctggc cgcctgctcg gtggtgcggg cgacgtaggt gaggcgctgg cgcgcgtcgg
    19561 gcacggcctc ggccgcccgg tgcagcgtgg tgtcgaggcc gagctcgcgc agcgcgtcat
    19621 ggacctggcg ggcgagctgg ccgagacggt tgtacaccac gccctcgcca gccgccgcgc
    19681 cggcattctc gaccgtgccg cccgcaaccg gcgccgcggc gccagcctgc tcggcgacgg
    19741 cgtcgaagag cgcctgcaac tcgtcgttat ccccggactc gtcgaccggc aggatcgatg
    19801 ccgccggctc cgcacccgca cccgccgcgg cgaaggggtc ggcggcaatg ctgtcgaaga
    19861 gcgcctgcaa ttcgtctgaa tcgcccatcg agtccacctt ggacttcttg ctcatatcca
    19921 tgctccgtct gcgctgcgga cgaggttcat gcccggccgc ggcccatctt ctcgaaaatc
    19981 ttctccagct tctcggccag cgtcgccgcg gtgaagggct tgaccacgta gccgctggca
    20041 ccggcctgcg cggcggcgat gatgttttcc ttcttggcct cggcggtgat catcagcacc
    20101 ggcaggtgct tgagctcggg cgtgcggcgg atgctctgca gcagggtcag gccgtccatg
    20161 ttgggcatgt tccagtcggt gacgacgaag tcgtaggggc tggacttgag cttctgcagc
    20221 gccaccgcgc cgtcctcggc ctcgtcgacg ttggcgaagc cgagctcctt caacaggttg
    20281 cgcacgatgc ggcgcatggt cgagaaatcg tcgacgacga ggaagcgggt cttgggatcg
    20341 gacatcagat gtctccgggt tctttgcctg ttcggtcagg aagtccgacg cgcccgttgc
    20401 agcatcgggc ggatggtgtc ggccaacact tcggcgtcga attttgccac gtaggcgtcc
    20461 accccgaccc gcgtgcccat ggcgcggttg gcctcggacg acagcgagga gtgcatgacc
    20521 accgggatgc cggcgaagcg cgtgtcgctc ttgatgcggc gggtgagcac gtagccgtcc
    20581 atctcgggca tctcggcgtc gagcatgatg aggtcgatct cgtcgatcag ccggtgcccg
    20641 gtgttctcgg cgtgggcggc caggctgtcg agccgcttcc acgcctccat gccgttggtc
    20701 gcgtgcttgt ggcgcacacc gaggcggtcg agcacctcgg tgatcttgcg gcgcgcgacc
    20761 atggagtcgt cgacgaagaa cacattcacc tcgtggccgt tgccgatcgg cgcgatgtcg
    20821 ccgaccaccg cctcgccgaa ggtgtcggcg aggatggact cgacatcgag gatcgacacc
    20881 aggcggccgt cgtcgagctc ggtgatcgcg gtgatgaagc cctgcgtgcc ggtcgacacg
    20941 ttctcgggcg cacgcacgcg ctcccagtcc acgcgcacga tgcggtcgac cgaatcgacc
    21001 aggaaaccga gcgtgcgctt gctgtattcg gtgaccatca tcgcctcgcc gagcggcgcc
    21061 cccgcagagg tcagcccgag gatgacgccg agcgagagca ccgggataac gtttccgcgc
    21121 agcgagatca ggccctcgac accgcgcggc atgttgggtg tgcgggtgat gaagggcgtg
    21181 cgcgagacct cgcgcacctt gaagacgttg atgccgaaat gctcgccggt accgagcgag
    21241 aacagcagga tctccatgcg gttcgagccg gccagcgtgg cgcggccatc gaccgcgtcg
    21301 aacagggcgg attcttcgtg acggttgtgt tccatggctc ttcctcagcg tccgctcgcg
    21361 gcctcggcca tgccgagcag ccgggtcagc acctgggaca ggcgctgcgg ttcgaacttg
    21421 gacacgtatt catccacccc gacagagagg ccgagctggt ggttcgagct gcccgacagc
    21481 gacgagtgca tgatcaccgg gatgccggcg aagcggcgat cggccttgat ctggcgggtg
    21541 agcatgaagc catccatctc gggcatctcg acgtcggtca gcaccaggct gacgaaatcg
    21601 gcgaccttgc gaccattggc ctcggcgcgc gccgcgaccc gggagagttc ctcccaggcc
    21661 tggcggccgt tgaccgcgcc gacgtggcgc aggcccatgg catccagggt ctgggagatc
    21721 tgcttgcgcg ccaccgagga gtcgtcggcg aaatacacca cgtgggcatc ggcacccggc
    21781 agcggcgcga tgtcgcggaa caggaaggcg tcgtcgtagc gcgaggtctc ggagagcacg
    21841 cgctcgacgt cgagcatcat caccaggcgg ccgtcggcga gcgaagtcac cgcggtcacc
    21901 agcccgccca ggcgcgcggt gagcatctcg ggcggcaccc gcatctgcga ccagtccagg
    21961 cgcaggatgg tgtcgacacc ctcgaccagg aagccctggg tgtggccgtt gtactcggtg
    22021 acgatcatga tctcgcgcgc agcatcgtca ccgaccccga cgtagcggcc gagatcgacc
    22081 accgggacga gcgcgccgcg caggctcacc atgccctgca ccgcgtcggg catgtcgggc
    22141 gcggcggtga tggtcggccg gcgcatcacc tcgcgcacct tgaagacgtt gatgccgtaa
    22201 gtctcgcggc agccgctgcg cgcgtccacg ccgagcgtga acagcaggat ctcgagcttg
    22261 ttggtgccgg cgagccgggt gcgggcgtcg atgttcttca gcagttcgga catggctgca
    22321 gccttggatt caattgggcg ccgggacaat ccccgggctt cccctcatat cggccggatt
    22381 gcgcccgact tgagcaccgc ccggcggcgg cgtggcgccg ggcgcctgct ggctgcgcat
    22441 cgcctcctcg gccgcacggt tgagcaccac gatgctgatg cgtcggttcg cggggtcgag
    22501 cggatcgccc ggattcaggt gcgcggtgtc ggcaaggccg accacgcgca gcatcttgcc
    22561 cgcctccagc ccgcccgcca cgagctcgcg gcgcgcggcg ttggcgcggt tggaggacag
    22621 ttcccagttc gagaagccgc gctcgccgcc tgcatattgg gccgagtccg tgtggccgga
    22681 catgctgaca cggttgtcga catcgttgag cgcagcggcg atctcgcgca gcagctcgcg
    22741 cgtatagggg cgcaactcgg cgctcgccga ggtgaacatc ggccggttct gcgcgtcgac
    22801 cagctgtatc cgcaagccct cgctgacgat gtcgatgagg agctggttct tgaactggcg
    22861 cagcgagggg ttggcctcga tgaccgcctc gagcttgccc ttgaggtcga tcaggcgcgc
    22921 gcgttcacgg cgctcggcga tcgcctgcgc ggtggcctcg gcctcgctct tgacgcgcgc
    22981 gggcgcggcg tcgagattga tcgcgcgcgt gctgggggta tcgccccggc gcacctggcc
    23041 gaccttgcgc gagaggtcct cgccaccgcc cttgatgatg ctcgagctgt cgccggtgcc
    23101 ttcgccgccc tgcatggcga gcttgagcgg gttctggaag tagtcggcga tgccctcgag
    23161 gtcgccctgc gcggtcgagc cgagcagcca catcagcagg aagaaggcca tcatcgcggt
    23221 gacgaagtcc gcgtaggcga tcttccatgc accgccgtgg tggccgccac cgcccttctt
    23281 gatgcgcttg acgacgatcg gttgctgggt atcgtcgctc atcgcgcaac tccgcacgcc
    23341 ggcgcagggc gcggcgcctg gggacgacgg gagtcaggga cgcgggggct catcgcgttc
    23401 agcccttgcg gttcttgatt tcctgctcga gctcggcgaa gcccgggcgg tcggtcgagt
    23461 acagcacctt gcggccgaac tccaccgcga cctgcggcgc atagccgttc atgctcgcca
    23521 gcagcaccac cttgatgacc tggtagatct tgccgctctc ctcgaccttc tgctcgagct
    23581 ggctggcgat cggcgcgacg aaaccatacg agaccaggat gccgaggaag gtgccgacca
    23641 gcgcgccgcc gatcatcttg cccagcaccg cgggcggctg acccaccgag cccatcacgt
    23701 tcaccacgcc catcaccgcc accacgatgc cgaaggcggg caccgcatcg gcgaccttgg
    23761 agaccacgtg cggcgcctgg tgcgcctcct ggtggtgggt ctcgatctcc atgtccatca
    23821 ggttctcgat ctcgaaggcg ttgaggttgc cgcccaccat catgcgcagg tagtcggtca
    23881 tgaactcgac cgcgtgatgg tcggcggcga cgctcgggta cttggagaag atcgggctgg
    23941 agtcggggtt ctcgatgtcg ttctcgatcg acatcaggcc ttccttgcgc accttggcga
    24001 ggatctcgaa cagcagcgcg agcaggtcga catagagcgc cttgttgtac ttcgagccct
    24061 tcaccgccag cgacagcgag ccgagcgtgc ccttgatcgc cttcgggccg ttgccggcga
    24121 cgaagccacc gatcatcagg ccgatgatcg cgaggtactc catgggcacc cacagcgcgg
    24181 ccaggctgcc atgcaccgcg taggtgccca gcgaggcggc gaggatgatg acgtagccga
    24241 tgatcaggaa cacggggaaa ctccgaagcg tttcgctgcg ccgcgcacgg accggcacgg
    24301 ctctcgcagg ggataacggc ggcggcggga ggaacttgaa cgcgccgggg cgcgacgcca
    24361 cgccgtgcgc ggacgtgaca tgggccgcct gtttccagcg cggcccatgt aggaacctgc
    24421 atgcgggccg gagggcccgc cggggtcagg cgacgaccgc cttgcggctg gcggccgtcg
    24481 tcttgtgcgt gggcgcgacc gccttgcggg tggtggtctt gcggcgcgcg gagccggcgc
    24541 gcgagggcat gttgcacagg ccgcacacgt agtcgtgcac cggatcgtgg gcgtgggcga
    24601 cgaactcgcc gccgcagcgg gtacacgcgg ccatctgcag caggtcggcg tcgaagaagc
    24661 gcaccagggt ccacgcgcgc gtgaggctca gcgcgggctc gatcacgccg gtctcgatgt
    24721 ggtcgagata gaggtgatag gacttgagga tcgcgtccag gccgtgcacg ccggccgggc
    24781 cggtgaagta gcggtacagg ttcatgaaca gcgaggaatg cacgttcggc tgccaggtca
    24841 tgaaccagtc ggtggagaac ggcagcatgc ccttgggggg cgaggcaccg cggacttcct
    24901 tgtagatcct gagcagcttc tcgcggctca gcctggtctc gacctcgagc atctgcatgc
    24961 gcgcaccgag ttgcaccatc tcgaccgcac gacgcacgtc gtcggcctct tcgatcacac
    25021 tcttg
//
LOCUS       DAOQBS010000034        24831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1273_length_24831_cov_15.393203, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000034 DAOQBS010000000
VERSION     DAOQBS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 24831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24831
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1273_length_24831_cov_15.393203"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PKN25_05885"
     CDS             <1..280
                     /locus_tag="PKN25_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90465.1"
                     /translation="LSSAPALAQKAPAAPAAAAAANPPTPEWRRTDGYRFPALADSVR
                     RLQYTTLAMQLREYCADRRVSDDFVRERLQRFGRITGRAETCASLRDY"
     gene            complement(274..2076)
                     /locus_tag="PKN25_05890"
     CDS             complement(274..2076)
                     /locus_tag="PKN25_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV90466.1"
                     /translation="MNPSGHLPGLPPSPGPSSAEPFVADLSEGAETGVYLALLELLDE
                     GLLITGDEVVLDANSAACRLLGRDYRDVAGRPLADLFPDGQAFLAARARLLIEGERRG
                     RLDFALPDGGVRALEFNCAPRLRPGIHAIILSAPAVVAEYETGTPVLAAPRPTAGGAR
                     AFDDAPSMSMYLTSHDPLTGLANRRMLEARFGDCAVRAAGRHGSIAIVRLDLDGFAAV
                     NRSLGESAGDEILRCIARRLAATAGSAALVARERSDSFIVLLPEPAHPGNADQFAETL
                     LRAVAEPLELGGGLQRITASAGLALYPQDGRNLDTLLRNAKSALEQARRLGGNHCSVF
                     HTRLDGPGFDSSHLERGLRHALQADELCLHFQPLVDARSGRTRAGEALLRWRHPELGL
                     LPYRQFMGAVGDPRLLADVGDWVLATACDHATDWPPTHDGECLRLTVNVSVEQLMRGD
                     FAARVATALRTSGLKAERLELDLDEKVLALDSPTLAATLERIAATGVRLSIDDFGRGL
                     SSIPRLRRYPLAALKLDPELVRGVGRSEDSEAIVEAVGALAGTLGLEIYARGVEHRGQ
                     QAFLCALDCHLQQGPLFGRPLAAREFADRLLRAQ"
     gene            complement(2201..3709)
                     /locus_tag="PKN25_05895"
     CDS             complement(2201..3709)
                     /locus_tag="PKN25_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV90467.1"
                     /translation="MSFADLGLIPELLQAVTDAGYTEPTPIQRQAIPIVIAGKDVMGG
                     AQTGTGKTAGFTLPLLHRIARHANTSTSPARHQTRALILAPTRELAMQVFESVKTYSK
                     HLPLRSVCVYGGVDIRPQQAELRRGIEIVIATPGRLLDHLEQKSINLSQVEVLVLDEA
                     DRMLDMGFIPDIKRILALLPKQRQSLLFSATFSDEIKRLADQMLKEPQLIEVARRNMV
                     SETITHVVHPVSSGMKRNLLAHLLRHKPDTQALVFVDTKLMCGRLAHYLERSGISADA
                     IHGDKGQQQRTDTLEAFKSGKLRVLVATDVAARGLDIDELPFVINFELPHTAEDYVHR
                     IGRTGRAGHHGYAISLVSSEEKHWLSEIEKLIKLQIPQERVPGFDPDPDFHEAGSRGH
                     RGRRPAAAAADTEAQAPAARSGGRERGVERSAEARREPAREREGLRAGPRRGRSSVAA
                     DGFDFSKPYEPLNPPVAVAEQTQPTVAAAGKPDPLRRHQRPIAVLLGGLGRK"
     gene            complement(3848..4942)
                     /gene="rlmM"
                     /locus_tag="PKN25_05900"
     CDS             complement(3848..4942)
                     /gene="rlmM"
                     /locus_tag="PKN25_05900"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HNV90468.1"
                     /translation="MNQHTPAAALACAGLLGYCRAGFEKELAAELDDIAAEAGLIGYV
                     RAEPDSGYVIYETFEPTPLGSFGESTDWRRPVFARQLLPWFARVDDLPERDRATPIVE
                     AVKASGQRFSGVMLETPDTDEAKQRSGFCKRFTEPLAKALEKAGCLRSSRAGLPVLHV
                     LFTSATTAWLAAGQPGQCSTWPMGIPRLRMPSNAPSRSTAKLSEAFMMLLEEGERDSI
                     LRAGQRAVDLGAAPGGWTWQLVNRGLRVTAIDNGPLRDSVMATEMVEHLKADGFTWRP
                     HRPVDWMVCDMVEQPSRIASLMAEWVATGRCRYTIFNLKLPMKRRVEAVEQCRELIRK
                     RLASVGPYDLRIKHLYHDREEVTAFLTLKR"
     gene            5242..6279
                     /locus_tag="PKN25_05905"
     CDS             5242..6279
                     /locus_tag="PKN25_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNV90469.1"
                     /translation="MEYRRLGGAGPQVSAVCLGTMTFGQQNSEAEAHSQLDLAVERGI
                     DFIDTAEMYPVPARAETSGETERIVGNWLARQVREKLVIATKVAGPARSLEWIRGGPL
                     ALDRANIRTAVEGSLRRLRTDYIDLYQLHWPARNQPLFGQLPFDPARERESTPIRAQL
                     EALAELVDEGKIRHVGLSNEQPWGLMEFVRMAQESGLPRVVSVQNAYNLLNRVYEYGM
                     SEITLREDVALLAYSPLAFGHLSGKYLADPAARGRLTEFENFGVRYAKPGVRAAVERY
                     AEIARRRGMSLTALALAFVYSRWFVGSTIVGATTVAQLAENLDAWHRRLDEDALAEIE
                     RVHQLHGNPAP"
     gene            complement(6301..6663)
                     /locus_tag="PKN25_05910"
     CDS             complement(6301..6663)
                     /locus_tag="PKN25_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90470.1"
                     /translation="MPRPTPSPTLRRLVLLALLPFAAGCDQLAEVLELPNPSKVEAEG
                     RAVGSACRHSGRSLEDCYALNPAASKAAVFAGWRDMNDYMMEHKLEIVPSQLVQGAPK
                     AAPPRSTAQAAPAQPAAH"
     gene            6839..8101
                     /locus_tag="PKN25_05915"
     CDS             6839..8101
                     /locus_tag="PKN25_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNV90471.1"
                     /translation="MNKMRFKALAAAVAAGGLFFAPGSVVQASAQQQVQLFHRLPDAK
                     AGALKDLVERFNAQSKDVQVVMSAADWRSGAPHLMILEGDDEEEFVAGKPRFKPLFQL
                     MKESGVPLQTLRPPAMMTRTPVDAKGQLLALPVGLSTPVLFLNRDALRQAGLNPETTR
                     INTWFDLQETLGRLADTGHTCPYTVAEPGRVMVENLSAWHNEPVAAQSGKTTVPSFNG
                     MFQVKHVAMMASWTRARYLHVFDQQAEAEQRFARGECAVIAAPSASWTDFRRAGKVDV
                     AVSKLPYYDDFPGAPQNTIADGPALWASAGKKPAEYKAVARFVSFWLQPDNQVAWQRE
                     TGYLPLNRAGLLASRSELLGNDLENIQVAVDQLGGKPATPQSSAQPVVERQKVRRILD
                     EELAGVWADEKAAKEALDNAVTRARNGN"
     gene            8197..9165
                     /gene="ispB"
                     /locus_tag="PKN25_05920"
     CDS             8197..9165
                     /gene="ispB"
                     /locus_tag="PKN25_05920"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HNV90472.1"
                     /translation="MSVQQLFAPIAADMQAVDTVIRNRLHSDVALIRRIAEYIVGSGG
                     KRLRPALVLYAAGALDYRGVHHHELAAVVEFIHTASLLHDDVVDESDLRRGKRTANAA
                     FGNASAVLVGDFLYSRAFQMMVGVDEMRVMRVLADATNIIAEGEVLQLLNCHNADVVI
                     EDYLRVIRYKTAKLFEAAARLGGIVAGADDALEQRLAAFGMHLGTAFQLIDDVLDYSA
                     DEADTGKHLGDDLAEGKPTLPLIHVMQHGTAEQGALVRHAIEGGGRGDFAAVLAAIQS
                     TGALEETRRYAEAEAKLAIDAISVLPPSIFKEALLQLSDFAVRRKH"
     gene            9212..9288
                     /locus_tag="PKN25_05925"
     tRNA            9212..9288
                     /locus_tag="PKN25_05925"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9246..9248,aa:Pro,seq:cgg)
     gene            complement(9575..10990)
                     /gene="pntB"
                     /locus_tag="PKN25_05930"
     CDS             complement(9575..10990)
                     /gene="pntB"
                     /locus_tag="PKN25_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HNV90473.1"
                     /translation="MTSSLATVSYIGATILFILSLGGLAHPETARRGNLFGIAGMSIA
                     VLATVFGPQVGMGGIPWIVIALVIGAAVGLYAAKKVQMTQMPELVALMHSLVGLAACL
                     VGFASYVDTSLVFPTAAEKTIHEVEIYVGILIGAVTFSGSLVAFAKLSGKIGGKPLLL
                     PARHWLNLAGLILVILLGRWFLNTHSIWAGMALLLVMTLIALAFGVHMVMAIGGADMP
                     VVVSMLNSYSGWAAAATGFMLGNDLLIVVGALVGSSGAILSYIMCRAMNRNFVSVIAG
                     GFGSGSGAPAATADGSEPAGEVTSISAVDTADLLRDAKNIVIVPGYGMAVAQAQHVVC
                     EIVNVLRGQGVAVRFAIHPVAGRMPGHMNVLLAEAKVPYDIVLEMDEINADFPETDVA
                     IVIGANDIVNPAAQEDPGSPIAGMPVLEVWKARTSIVMKRSMASGYAGVDNPLFYKEN
                     NRMLFGDARKMLDEVLGALSA"
     gene            complement(11001..12590)
                     /locus_tag="PKN25_05935"
     CDS             complement(11001..12590)
                     /locus_tag="PKN25_05935"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNV90474.1"
                     /translation="MTLLIGVPKETFAGEKRVATVPEVVAKLIKLGFSVAVESGAGAA
                     ANFSDDDYRAAGAEVSPGSAELLGRADIVFKVRPPTLDEVGMLREGATLIGFVWPAQH
                     PELMAALQARKTTVLAIDCLPRQLSRAQKMDALSSMAGISGYRAVVEAANAFGRFFNG
                     QITAAGKIPPAKVFIAGAGVAGLAAIGTAANLGAVVRANDTRAEVADQVVSLGGEFVK
                     VDYDEEGSGGGGYAKVMSEGFQAAQRQMYAREAKDADIVITTALIPGKPAPKLITAEM
                     VQSMKPGSVIVDLAAEQGGNCELTVPGEAVVRHGVTIIGYTDLVSRLARQSSTLYSNN
                     LLRLTEDLCKAKDGNIAVNFEDDAIRGLTVIKQGELTWPAPPPKLPAASAKPKASSAP
                     ITAPKGHGHGAGAPMPVKSLAIVFGVGALLFLLVGLYAPASFLTHFTVFVLACFVGYM
                     VVWNVTPSLHTPLMSVTNAISSIIAIGALVQIAPPLAEGASDRPGGLIGVLAFVAITL
                     TAVNMFGGFAVTRRMLAMFRK"
     gene            complement(12832..13071)
                     /locus_tag="PKN25_05940"
     CDS             complement(12832..13071)
                     /locus_tag="PKN25_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90475.1"
                     /translation="MAKQTIKNPPSAAHDGDLICFERHAVPGADARMLTPGRGLASRC
                     IGGGPGIALAIESQVGSGILAKGTNIGNRRECDRP"
     gene            complement(13147..13326)
                     /locus_tag="PKN25_05945"
     CDS             complement(13147..13326)
                     /locus_tag="PKN25_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90476.1"
                     /translation="MLKPSICSAMVSDRRARLGCTTALNDAPIDMMTISLDSDVSALL
                     RQVRVARGTPHIPRP"
     gene            13420..13995
                     /locus_tag="PKN25_05950"
     CDS             13420..13995
                     /locus_tag="PKN25_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HNV90477.1"
                     /translation="MNPKPRHIERALRERVEIVPYDPAWPGLFAEEVARLRAALPAEL
                     IGRIEHFGSTAVPGLAAKPIIDMLVEVRSMEDVAERIAPLLRALGYEFFWRAPGHGLP
                     GMEYAWFIRRDAAGRRTHHIHFVEAGSSEWGRLRFRDYLRAHSEVARAYGALKQRIAD
                     QYPDDRIAYAKAKTCFVREVMRAVRRDHIPW"
     gene            complement(13980..15617)
                     /locus_tag="PKN25_05955"
     CDS             complement(13980..15617)
                     /locus_tag="PKN25_05955"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HNV90478.1"
                     /translation="MERESMEFDVLVVGGGPAGLAAAIRLKQLAEAKGSELSVCLIEK
                     GAEIGAHILSGAVIDPRSLNELIPDWKDKGAPLTTAVTEDQVLFLSETGVRRVPPALT
                     PDCFENHGNYIVRLGNLVKWLGEQAEAAGIEVYPGFAAAEILYDESGAVKGVATGDMG
                     LNRDGTPGPHHQPGMELHAKYTLFAEGCRGHLGKQLEAKYKLREGVDPQTYGIGIKEL
                     WEVKPEQHRPGLVVHTAGWPMDNRTYGGGFIYHLEDNLVALGFVVGLNYENPHLSPFD
                     EMQRWKTHPEVRKHIEGGKRLAYGARAIATGNIQSLPRLIFPGGGLIGDDAGFLNAAR
                     IKGTHTAIKSGMLAAEAAFDALAANRQRDALTAYPAAFRDSWLYAELHKYRNFKPLMK
                     KGLWMGSFLFGMDQMLFRGKAPWTLHNSSDHDKLKPASECPKIDYPKPDGVLTFDRLS
                     SVFLSNTNHEEEQPCHLQLKDPSVAIAVNLAKYDAPEQRYCPAGVYEIVREESGPRLQ
                     INAQNCIHCKTCDIKDPTQNINWVVPQGGEGPIYQGM"
     gene            15807..16226
                     /locus_tag="PKN25_05960"
     CDS             15807..16226
                     /locus_tag="PKN25_05960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNV90479.1"
                     /translation="MSPNAKLIYTTRMPVRWGDMDAYGHVNNTVYFRYFEQTRVEWLE
                     RMGSRVSVEEPVGPVIINASCTFLAPVNYPATVVIKMYAGDPGRSSVMTWYELFVDGD
                     ERLYAEGAAKTVWMDMRTGKSAPIPDVVRALFDPPVA"
     assembly_gap    16389..16488
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16515..18503
                     /locus_tag="PKN25_05965"
     CDS             16515..18503
                     /locus_tag="PKN25_05965"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNV90480.1"
                     /translation="MYPTEQKPLWTPSPERIAAANVTAFRLAAEQRWKVSLPDYEALH
                     AWSVEHPEPFWTSVWEGDGVGGGVIGTRGERVLVDGHKMPGARWFPDARLNFAQNLLR
                     SRDAHDAIVFWGEERVKNRLSHGELYRAVAHFAAALKEQGVVAGDRVAAYMPNLPETV
                     VAMLAAASLGAIFTSASPDFGVQGVLDRFGQTEPKVLVACDGYYYGGKTVDVLGKLGE
                     IVAQLPSVKRVVVVPYVHREHDLLHVPHARMLADFVAPFHFVDDIEFAQLPFDHPLYI
                     MYSSGTTGVPKCIVHCAGGALLQHLKEHKLHGDVKPGDRVFYFTTCGWMMWNWLVSAL
                     AAEATLLLYDGSPFAGDNRILFDYADAEAMTHFGTSAKFLDAAAKFGLKPRETHKLDT
                     VRAMMSTGSPLVPEGFDYVYRDIKADLQLSSISGGTDIISCFVLGSPVLPVWRGEIQC
                     RGLGMAVDVWDDDGRPVRGEKGELVCAKPFPVMPIGFWNDADGSKYHAAYFDRFPNVW
                     CHGDFCEITAHGGLVIYGRSDATLNPGGVRIGTAEIYRQVEKLHEVVESLVIGQDWPP
                     QNPNDVRVVLFVKLREGLALDDDLTRRIRQTIRDNTTPRHVPAKVLQVADIPRTKSGK
                     IVELAVRNVVHGRPVKNQEALANPEALAHFRDRAELQG"
     gene            18518..19060
                     /locus_tag="PKN25_05970"
     CDS             18518..19060
                     /locus_tag="PKN25_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNV90481.1"
                     /translation="MLMNPKKSVLLVIDVQAKLAPFIHDNAAVVEACVWLARVAERIG
                     VPVVVTEHFPEKIGGTVEAVRAATAGAHYVGKQFFSAQADGCLDQTAVAGCEQVILCG
                     TEAHVCVQQTALDLRGAGKQVFIVAEASGSRAPANRDLAFERMRGHGIEIVSREMVAF
                     EWLQRGGTELFREVNRDFIR"
     gene            19277..24106
                     /locus_tag="PKN25_05975"
     CDS             19277..24106
                     /locus_tag="PKN25_05975"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HNV90482.1"
                     /translation="METREAGRLATQVEAVIEQIRTRMPAEQAEGVCAFASRFFAQVA
                     PEDLEDLPVGDLYGAVLSQWHFVARRQDGSKVRAFNPRLDEHGWECPHTVVEIAGEDM
                     PFLVDSITMEVARQGLTLHLIIHPVMNVARDAEGGFVRVAERGEQGEGVGFESIMHLE
                     VDRRTDPGDLVALQQGIEHVLADVRAAVRDWSAMRERLAGITAGLDELPVSIDAEETQ
                     EVRAFLDWLAADNFVLLGCRDYALVETGEGNELRIVPGSGLGLLRGDGDEGQSRSFAA
                     LPPQLRAQAHLPHLLTVTKSNSRSTVHRPGYLDFVGIKTFGADGRVNGERRVIGLLAS
                     TAYSASPRQIPLLRRKVEAVFERAGLLPGGHAAKALQTLLERYPRDELFQIETDELHA
                     HAMGILRLGERLRTRLFVRVDPFERFVSCLIYVPREHYNTDQRERMQAVLIDAFKGNA
                     AEFDVQFSDSALARILITVRTPEGRIPAFDVREIEQRLIRAARRWEDELQQALVEQCG
                     EERGLALMRRYGEGFPAGYREEYSARMAVFDIEQMEALADDAALGLNLYVPLEVRAGR
                     LNLRLYHLGSPVPLSQSLPMLEKMGVKVMDERPSEIERQDGSTVWLHDFGLQFAGAEN
                     LNIHDIRPLFQEAFLAAWRGAAENDDFNRLVLLAGLSWREVAVLRAYARHMRQAAFTF
                     SLAYMEQTLAAYPQFARALLQLFRARFDPALAGDREAACATQVAAIEAALDQVANLDE
                     DRILRQFLALMQATLRTNWFQRGADGAPKPYLSFKFLPSKIPNLPQPLPMFEIFVYSP
                     RFEGVHLRGGKVARGGLRWSDRMEDFRTEILGLVKAQIVKNAVIVPVGSKGGFVVKCP
                     PAEGGREALLAEGVACYRNFLRGLLDLTDNLVQGAVVPPADVVRHDEDDPYLVVAADK
                     GTASFSDYANQVSAEYGFWLGDAFASGGSVGYDHKKMGITARGAWEAVKRHFREMGKD
                     IQQEPFTVVGIGDMSGDVFGNGMLLSKQIRLVAAFDHRHIFIDPDPDPARSWEERARM
                     FALPRSSWDDYDRALISQGGGVWPRSAKSITLSPEARAALDIQAAPLGLTPTELIRAI
                     LTAPVELVYNGGIGTYIKAASQTDAAVGDRANDAVRVNGGELRCKVFGEGGNLGATQL
                     GRIEFALAGGRINTDAIDNSGGVDCSDHEVNIKILLGGVIAEGELTLKQRNELLADMT
                     DEVGILVLRDNYAQTQVLSVTRARGVALLDEQAEFIRRLVQAGRLNRKLEYLPDDEEI
                     AERKAAGVGLTSPELAVLLAYSKIELYDEVLASDVPEDPYIRTALERYFPGPLRERFP
                     AQIRIHPLRREIISTHVVNSMINRVGPTFVSRLRAELGASAADVVRAYMATREVFGLV
                     ALWREIEALDNRIADAVQTELIQESGRLVQRGTLWFLRHRRWLADLQATSAHFSPGVA
                     ALAEGLADYVAPAYRAELDAAVARRVEQGVPEALAKRVAALEELYSALDLVEVAVEQG
                     RDEATVARVYFALGGEFDLHWLGRQISGLPADTRWQSLARGALRSDLSSLARELTSAA
                     LRNAPEGADTEGVLNAGRERAAVPLERYQQLLAEIRSAPTIDMAMLSVLLRELRGMA"
     gene            24189..>24831
                     /locus_tag="PKN25_05980"
     CDS             24189..>24831
                     /locus_tag="PKN25_05980"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNV90483.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTSAKMSKRNNFTTGQIYESVLKKE
                     RRGEYLGKTVQVIPHITDEIKAYVKRGAEGADVAIVEVGGTVGDIESLPFLEAIRQMG
                     FEEGRHGTCFIHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADRP"
BASE COUNT         3754 a         8335 c         8782 g         3860 t
ORIGIN      
        1 cctttccagc gctccggccc ttgcgcagaa ggctcccgct gcgcctgcag ctgcggctgc
       61 ggcgaatccg ccgacgcccg agtggcggcg caccgacggc tatcgtttcc cggcgctcgc
      121 cgacagcgtg cgccgcctgc aatacaccac gctggcgatg cagctgcgcg aatactgtgc
      181 cgaccggcgc gtgtccgacg acttcgtgcg cgagcgcctg cagcgcttcg gtcgtattac
      241 cgggcgcgcg gagacctgcg ccagcctgcg ggattactga gcgcggagca ggcggtccgc
      301 gaactcccgc gccgccagcg gccgcccgaa cagcggccct tgctgcaggt ggcagtcgag
      361 cgcgcacagg aaggcctgct ggccgcggtg ctcgaccccg cgcgcgtaga tttccagccc
      421 gagcgtgccg gcgagcgcgc cgaccgcctc gacgatggcc tcgctgtcct cgctgcgacc
      481 gaccccgcgt accagctcgg ggtcgagctt gagcgcggcg agcgggtagc ggcgcaggcg
      541 cgggatggac gacaggccgc ggccgaagtc gtcgatcgac aggcgcacgc cggtggcggc
      601 gatgcgctcc agcgtggcgg cgagcgtcgg gctgtcgagt gccagcacct tttcgtcgag
      661 gtcgagctcg agccgctccg ccttcagccc ggaggtgcgc agggcggtcg cgacgcgcgc
      721 cgcgaagtcg ccgcgcatga gctgctcgac cgagacattc accgtcaggc gcaggcattc
      781 gccatcgtgc gtcggcggcc agtcggtggc gtgatcgcag gcagtggcca gcacccagtc
      841 gccgacgtcg gcgagcaggc gggggtcgcc gaccgcaccc atgaactggc ggtagggcag
      901 caggccgagc tcggggtggc gccagcgcag caaggcctcg cccgcacggg tgcgaccgga
      961 gcgcgcatcg accagcggct ggaagtgcag gcacagctcg tcggcttgca aggcgtggcg
     1021 caggccgcgt tcgagatggc tgctgtcgaa gccggggccg tcgagccggg tgtggaagac
     1081 gctgcagtgg ttgccgccga gccggcgcgc ctgttcgagc gcgctcttgg cgttgcgcag
     1141 cagggtgtcg aggttgcgcc cgtcctgcgg atagagggcg aggccggcgc tggcggtgat
     1201 gcgctgcagc ccgccgccga gctcgagggg ctcggccact gcgcgcagca gggtttcggc
     1261 gaactggtcg gcgttgccgg gatgcgccgg ctcgggcagc agcacgatga agctgtcgct
     1321 gcgctcgcgc gccaccaggg cagcggaacc ggcggtagcg gcgaggcggc gggcgatgca
     1381 ccggaggatc tcgtcgccgg cgctctcgcc caggctgcga ttgaccgcgg cgaagccgtc
     1441 gaggtcgagg cgcacgatcg cgatgctgcc gtggcggccc gccgcgcgca ccgcgcagtc
     1501 accgaagcgc gcctcgagca tgcggcggtt ggccaggccg gtgagcgggt cgtggctcgt
     1561 caggtacatc gacatcgacg gcgcgtcgtc gaacgcgcgc gcaccgcctg ctgtcgggcg
     1621 tggcgctgcg aggacggggg tgcccgtttc gtactccgcc acgactgcgg gcgcgctcag
     1681 gatgatggcg tggatgccgg ggcgcagccg cggcgcgcag ttgaactcga gcgcgcgcac
     1741 gccgccatcc gggagagcga agtcgaggcg gccgcggcgt tcgccctcga tcagcaggcg
     1801 cgcgcgcgcg gccaggaagg cctggccgtc ggggaagagg tcggcgagcg gacgccctgc
     1861 cacgtcgcgg tagtcgcggc cgagcaggcg gcaggcggcg ctgttggcgt cgagcacgac
     1921 ctcgtcgccg gtgatcagca ggccctcgtc gagcagttcg agcagggcga ggtatacgcc
     1981 ggtctcggcg ccctccgaaa ggtccgcgac gaagggctcg gcagaggagg ggccggggga
     2041 gggcggcagg cccggcaggt gtccggaagg gttcacgaca gcagaggatc gcaggggagt
     2101 tcgatgcggg cgcccccggg cgggggagcc tgcgagtgag accacagcat agccgatgcc
     2161 tgtgcgccgc cgtgtggcga ataaggggcc ggggtgcccc ttatttgcgc ccgaggccgc
     2221 cgagcagcac cgcgatcgga cgctggtggc gacgtagcgg gtcgggcttg ccggcagcgg
     2281 ccacggtggg ctgagtctgc tcggcgaccg cgacgggggg attgagcggc tcgtagggct
     2341 tggagaagtc gaagccgtcg gcggcgacgc tgctgcggcc gcggcggggg ccggcgcgca
     2401 ggccttcgcg ctcgcgcgct ggctcgcgac gagcctcggc gctgcgctcg acgccacgtt
     2461 cgcggccgcc ggagcgcgcc gccggagcct gcgcctcggt gtcggcggcc gcggccgccg
     2521 gcctgcggcc acgatggccg cggctgccgg cctcgtggaa gtcgggatcg gggtcgaagc
     2581 cgggtaccct ctcctgcgga atctgcagct tgatcagctt ttcgatctcg gacagccagt
     2641 gcttttcttc cgagctgacc agcgagatcg catagccgtg gtggccggcg cggccggtgc
     2701 gaccgatgcg gtgcacgtag tcctcggcgg tgtgcggcag ctcgaagttg atcacgaagg
     2761 gcagctcgtc gatgtcgagg ccgcgcgcgg ccacgtcggt ggcgaccagc acgcgcagct
     2821 tgcccgactt gaaggcctcg agcgtgtcgg tgcgctgctg ctggcccttg tcgccgtgga
     2881 tggcgtcggc cgagatgccg ctgcgctcga ggtagtgcgc caggcggccg cacatgagct
     2941 tggtgtcgac gaagaccagg gcctgggtgt cgggcttgtg gcgcagcagg tgggcgagca
     3001 ggttgcgctt catgcccgag gacaccgggt gcaccacgtg ggtgatggtc tcggagacca
     3061 tgttgcggcg cgcgacctcg atcagctgcg gttccttcag catctggtcg gcgaggcgct
     3121 tgatctcgtc cgagaaggtg gccgagaaca gcaggctctg gcgctgcttg ggcagcaggg
     3181 cgaggatgcg cttgatgtcg gggatgaagc ccatgtcgag catgcggtcg gcttcgtcca
     3241 gcaccagcac ctcgacctgc gacaggttga tcgacttctg ctcgaggtgg tcgagcagcc
     3301 ggcccggcgt ggcgatgacg atctcgatgc cgcggcgcag ctcggcctgc tgcgggcgga
     3361 tgtcgacgcc gccatagacg cacaccgagc gcagcggcag gtgcttgctg taggtcttga
     3421 ccgactcgaa cacctgcatc gcgagctcgc gcgtgggcgc gaggatcagc gcgcgcgtct
     3481 ggtgacgggc gggcgaggtg ctggtgttgg cgtggcgggc gatgcggtgc agcagcggca
     3541 gcgtgaagcc ggcggtcttg ccggtgcccg tctgggcgcc gcccatgacg tccttgccgg
     3601 cgatgacgat ggggatggcc tggcgctgga tcggcgtggg ctcggtgtag ccggcgtcgg
     3661 tgacggcctg cagcagttcg ggaatgaggc cgagatcggc aaagctcatg ggtgccttcg
     3721 ggttggagac ggtcgacagt cgaccgccgg atgacggaaa acccggatta tacacggcac
     3781 aaaaagcgtt accggacagg ggcttcgccg ccgaccgcgc ggcggcggtc cgccgcgcct
     3841 ggtccgctca gcgcttgagc gtgaggaagg cggtgacctc ctcgcggtcg tggtagaggt
     3901 gcttgatgcg caggtcgtag gggccgacgc tggcgagccg cttgcggatc agctcgcggc
     3961 actgctcgac cgcctcgacg cggcgcttca tcggcagctt gaggttgaag atcgtgtagc
     4021 ggcagcgccc ggtggcgacc cattcggcca tcagcgaggc gatccgcgag ggctgctcga
     4081 ccatgtcgca caccatccag tccaccggcc ggtgcgggcg ccaggtgaag ccgtcggcct
     4141 tgaggtgctc gaccatctcg gtcgccatca cgctgtcgcg cagcgggccg ttgtcgatcg
     4201 cggtgacgcg caggccgcga ttgacgagct gccaggtcca cccgcccggc gcggcgccga
     4261 gatcgaccgc gcgctggccg gcgcgcagga tgctgtcgcg ctcgccttcc tcgagcagca
     4321 tcatgaaggc ttcggacagc ttggcggtgg agcggctggg cgcgttcgag ggcatgcgca
     4381 ggcgcgggat gcccatcggc caggtcgagc actgcccggg ctggccggcg gcgagccagg
     4441 cggtggtggc gctggtgaag agcacgtgca ggaccggcag gccggcgcgg ctgctgcgca
     4501 ggcagccggc cttctcgagc gccttggcga gcggctcggt gaagcgcttg cagaagcccg
     4561 agcgctgctt ggcctcgtcg gtgtccggag tctccagcat caccccggag aagcgctggc
     4621 cgctggcctt caccgcctcg acgatcggtg tggcgcggtc gcgctcgggc agatcgtcga
     4681 cgcgcgcgaa ccacggcagg agctggcgcg cgaacaccgg gcggcgccag tcggtggatt
     4741 cgccgaagct gccgagcggg gtgggctcga aggtctcgta gatcacgtag cccgagtcgg
     4801 gctcggcgcg cacgtagccg atcaggccgg cttcggcggc gatgtcgtcg agttcggcgg
     4861 cgagttcctt ctcgaagccg gcgcggcaat agccgaggag gccggcgcag gcgagcgcgg
     4921 cggcgggggt gtgctggttc atggggtcgg tgcgcgcagg ggctgcagta tgatgggtcc
     4981 gtgccgggcc gcaggggcgg caaccggaat cggcgcaagg cagcggatga taagccgcga
     5041 gcgccgcgct ggcgagcatg ccggcgctac gacgcaggtt gagatcgggc cggccgccgc
     5101 gcctgcgcgg gttccgcgcg ccgcagttgc gattcgcgtc gctcccaagg cacaccctcg
     5161 cgcctccgga gcagcccgcg ggagcgcggc gggtcacgat tcggttgtcg gaaaagggga
     5221 tacgggatgg cagtacgtga aatggaatac cgccggctgg gcggagcggg gccgcaggtg
     5281 tcggcggtgt gcctgggcac catgaccttt ggccagcaga acagcgaggc cgaggcgcac
     5341 agccagctcg acctcgccgt ggagcgcggg atcgacttca tcgacaccgc cgagatgtat
     5401 ccggtcccgg cgcgtgccga gaccagcggc gagaccgaac gcatcgtcgg caactggctg
     5461 gcgcgccagg tgcgcgagaa gctggtgatc gcgaccaagg tggcggggcc ggcgcgcagc
     5521 ctggagtgga tccgcggcgg tccgctcgcg ctcgaccgcg ccaacatccg caccgcggtc
     5581 gagggcagcc tgcgccggct gcgcaccgac tacatcgacc tgtatcagct gcactggccg
     5641 gcgcgcaacc agccgctgtt cggccagctg cccttcgatc cggcgcgcga gcgcgaatcc
     5701 acgccgatcc gcgcccagct cgaggcgctc gccgaactgg tggacgaggg caagatccgc
     5761 cacgtcggcc tgtcgaacga gcagccctgg gggctgatgg agttcgtgcg catggcgcag
     5821 gagagcggcc tgccacgcgt ggtgtcggtg cagaacgcct acaacctgct caaccgggtg
     5881 tacgagtacg gcatgagcga gatcacgctg cgcgaggacg tggcgctgct ggcgtattcg
     5941 ccgctcgcct tcggccacct gtcgggcaag tatctcgccg acccggctgc gcgcgggcgc
     6001 ctcaccgagt tcgagaattt cggtgtgcgc tacgccaagc ccggtgtgcg tgcggcggtg
     6061 gagcgctacg cggagatcgc gcggcggcgc gggatgagcc tcaccgcgct ggcgctcgcc
     6121 ttcgtgtatt cgcgctggtt cgtgggcagc accatcgtgg gcgcgaccac ggtggcgcaa
     6181 ctcgccgaga acctcgacgc ctggcatcgg cgtctcgacg aggatgcgct cgccgagatc
     6241 gagcgggttc atcagctgca cggcaacccg gcgccctgag cgtcggtgcg ggtacggcgg
     6301 tcagtgcgct gcgggctgtg ccggcgccgc ctgcgccgtg gaccggggag gcgctgcctt
     6361 tggcgcgccc tggacgagct gcgagggcac gatctcgagc ttgtgctcca tcatgtagtc
     6421 gttcatgtca cgccagccgg cgaacaccgc cgccttcgag gccgccggat tgagcgcgta
     6481 gcagtcctcc agcgagcgcc cggagtggcg gcaggcgctc ccgaccgcac ggccctcggc
     6541 ctcgaccttc gatggattgg gcagctcgag cacctcggcg agttggtcgc agccggctgc
     6601 gaagggcagc agggcgagga ggacgagtcg gcgcagcgtc ggggatgggg tcgggcgggg
     6661 catggcgatc cgggggcatt cttctggtgg ggttatcggc cgcggccacc ccatcttgag
     6721 aggagtgtcg cgcgcgcggc acgctcgccc tcgaggccca ctattcgagt gcgcgcggat
     6781 ctataatcgc gcccgcttcc gggccgtcct gcccggctcc tttctgtgtg tcgaatcgat
     6841 gaacaagatg cgtttcaagg ccttggccgc agcggtcgct gccggcggtc tcttcttcgc
     6901 gcccggctcg gtggtccagg ccagcgccca gcagcaggtc cagctcttcc atcgcctgcc
     6961 cgatgccaag gcgggggcgc tgaaggattt ggtcgagcgc ttcaacgcgc agtccaagga
     7021 cgtgcaggtc gtgatgtccg cggcggactg gcgctcgggc gcgccccacc tgatgatcct
     7081 cgagggcgac gacgaggagg agttcgtcgc cggcaagccg cgcttcaagc cgctcttcca
     7141 gctcatgaag gaaagtggcg tgccgctgca gaccctgcgt ccgcccgcga tgatgacgcg
     7201 caccccggtc gatgccaagg gacagctgct ggcgctgccg gtcggcctgt cgaccccggt
     7261 gctgttcctc aaccgcgacg cgctgcgcca ggccggcctc aacccggaga ccacccggat
     7321 caacacctgg ttcgatctgc aggaaaccct cgggcgcctc gccgataccg gtcacacctg
     7381 cccctatacc gtggccgagc ccggccgcgt gatggtggag aacctctcgg cctggcataa
     7441 cgagccggtg gccgcgcaga gcggcaagac caccgtaccg agcttcaacg gcatgtttca
     7501 ggtcaagcat gtggcgatga tggcgagctg gacgcgtgcg cgctacctgc acgtgttcga
     7561 ccagcaggcc gaggccgagc agcgcttcgc gcgcggcgag tgcgcggtga tcgccgcgcc
     7621 ctcggcgagc tggaccgact tccgccgtgc cggcaaggtc gatgtggcgg tgtccaagct
     7681 gccctactac gacgacttcc ccggtgcgcc gcagaacacc atcgccgacg gtcccgcact
     7741 gtgggcctcg gcaggcaaga aaccggccga atacaaggcc gtggcgcgct tcgtgagttt
     7801 ctggctgcag cccgacaacc aggtcgcgtg gcagcgcgag accggctacc tgccgctcaa
     7861 ccgcgccggc ctgctggcct cgcgcagcga gctgctcggc aacgacctcg agaacatcca
     7921 ggtcgcggtc gaccagctcg gcggcaagcc cgccacgccg cagtcgtcgg cgcagcccgt
     7981 ggtcgagcgc cagaaggtgc gccgcatcct cgatgaggaa ctcgccggcg tatgggccga
     8041 cgagaaggcc gcgaaggaag cgctcgacaa cgcggtgacg cgcgcccgca acggcaactg
     8101 agctcgctac cgctcacctg cggcgcccgg ggttgcggct tgacgccgcg cgcgggcgcc
     8161 gcctaacatc tacgattttc cgcgagccag aacgccttgt ccgtccagca gctcttcgcg
     8221 ccgatcgccg ccgacatgca agcggtggat acggtcatcc gcaaccgact gcactcggac
     8281 gtggccctga tccgccgcat cgcggaatac atcgtcggca gcggcggcaa gcggctgcgc
     8341 cccgcgctgg tgctctacgc ggcgggcgcg ctcgactatc gcggcgtgca tcaccacgag
     8401 ctcgccgcgg tcgtcgagtt catccacacc gccagcctgc tgcacgacga cgtcgtcgac
     8461 gagtccgacc tgcgccgcgg caagcgtacc gccaacgccg ccttcggcaa cgcctctgcc
     8521 gtgctggtgg gcgacttcct ctattcgcgt gccttccaga tgatggtcgg ggtcgacgag
     8581 atgcgcgtga tgcgcgtgct cgccgacgcc accaacatca tcgccgaggg cgaggtgctg
     8641 cagctgctca actgccacaa cgccgacgtc gtcatcgaag actacctgcg cgtgatccgc
     8701 tacaagaccg ccaagctctt cgaggccgcg gcgcgcctgg gcggcatcgt ggccggtgcg
     8761 gacgacgcgc tcgagcagcg cctggccgcc tttggcatgc accttggcac cgccttccag
     8821 ctcatcgacg acgtgctcga ctactccgcc gacgaggccg acaccggcaa gcaccttggg
     8881 gacgacctcg ccgaaggcaa gccgacgctg ccgctgatcc acgtgatgca gcatggcacg
     8941 gcggagcagg gcgcgctggt gcgtcacgcg atcgaaggcg gcggccgcgg cgatttcgcg
     9001 gccgtgctcg cggcgatcca gtcgaccggc gcgctcgaag agacgcgccg ctacgcggaa
     9061 gcggaggcga agctcgcaat cgacgcgatt tcggtgcttc ccccttccat tttcaaggaa
     9121 gcgctgctac aattatcgga ctttgcagtt cggcgaaaac actgatatag tttctggctt
     9181 cgcagggcag caaaaagcag caaaaaagtg tcggggaata gctcagcctg gtagagcact
     9241 gcgttcggga cgcaggggcc ggaggttcga atcctctttc cccgaccaaa aatcccgcaa
     9301 aaaagccact caatgagtgg cttttttgtt atcgaatcaa gggtttgcgt ttgattgccc
     9361 ccttgatgct gcgtcccggt gtcgcgctga tccgctttag cgcgacacga aatctgcact
     9421 gcagcgcgcc cggatctgac tctccaggtt ggccgtacga ttgtccgggc aggatccacg
     9481 tcccgattgc agccaccgcg gctcaggtcg ggcgggtttc accgcggtgc cgccgccacg
     9541 cgaaaccccg gcacgcggat ccgcccgtgc tgcttcaagc gctcagcgcg cccaacacct
     9601 cgtcgagcat cttgcgggcg tcgccgaaca gcatgcggtt gttttccttg tagaacagcg
     9661 ggttgtcgac cccggcgtag ccggaagcca tgctgcgctt catgacgatc gaggtccgtg
     9721 ccttccacac ctcgagcacc ggcatgccgg cgatcgggct gcccgggtct tcctgcgcgg
     9781 ccgggttcac gatgtcgttg gcaccgatca cgatcgccac atccgtttcg gggaagtccg
     9841 cgttgatctc gtccatctcg agcacgatgt cgtagggaac cttggcctcg gccagcagga
     9901 cgttcatgtg gccgggcatg cggcctgcca ccgggtggat ggcgaagcgc acggcgacgc
     9961 cctgtccgcg cagcacgttg acgatctcgc agacgacgtg ctgtgcctgt gcgaccgcca
    10021 tgccgtagcc cggcacgatg acgatgttct tcgcgtcgcg cagcaggtcg gcggtatcca
    10081 ccgcgctgat cgacgtgacc tcgccggccg gctccgatcc gtcggccgtc gccgcgggcg
    10141 cgccgctgcc ggagccgaag ccgcccgcga tgacgctgac gaagttgcgg ttcatcgcgc
    10201 ggcacatgat gtaggacagg atcgcccccg aggagcccac cagcgcgccg acgacgatca
    10261 gcagatcgtt gcccagcatg aagcccgtcg ccgccgccgc ccaacccgaa tagctgttga
    10321 gcatcgagac cacgacgggc atgtcggctc caccgatcgc catcaccatg tgcacgccga
    10381 aggccagcgc gatcaacgtc atcaccagca gcaaggccat gccggcccag atcgagtgtg
    10441 tgttgaggaa ccagcgcccg agcaggatca ccaggatcag cccggcgagg ttgagccagt
    10501 ggcgcgcggg cagcagcagc ggcttgccgc cgatcttgcc ggagagcttg gcgaaggcga
    10561 ccagcgagcc ggagaaggtg actgcgccga tcaggatgcc gacgtagatc tccacctcgt
    10621 ggatggtctt ctcggctgcg gtggggaaca ccagggaggt gtccacgtag ctcgcgaagc
    10681 ccaccaggca ggccgccaga cccaccaggc tgtgcatgag ggcgacgagc tcgggcatct
    10741 gcgtcatctg caccttcttc gcggcgtaga ggccgacggc ggcgccgatg acgagggcga
    10801 tgacgatcca tggaatgccg cccatgccga cttgcgggcc gaacacggtg gccagcaccg
    10861 cgatgctcat gcccgcgatg ccgaacaggt tgccgcggcg cgcggtctcg ggatgggcga
    10921 gtccgcccag gctgaggatg aagaggatgg tggcgccgat gtacgagaca gtggccagac
    10981 ttgaggtcat tgtcttctcc ttatttgcgg aacatcgcca gcatgcgacg cgtcaccgcg
    11041 aagccgccga acatgttgac ggccgtgagg gtgatggcga cgaaggcgag cacgccgatc
    11101 agcccccccg gtcgatcgct ggcgccttcg gccagcggtg gggcgatctg cacgagcgcg
    11161 ccgatcgcga tgatcgacga gatggcgttg gtgacgctca tcagcggcgt gtgcagcgag
    11221 ggtgtgacgt tccagaccac catgtagccc acgaagcagg ccagcacgaa cacggtgaag
    11281 tgcgtgagga agctggccgg ggcgtacaga ccgacgagca ggaacagcag ggcgccgaca
    11341 ccgaacacga tcgccagcga cttgaccggc atcggcgcgc cggccccgtg gccatggccc
    11401 ttgggtgcgg tgatcggtgc gctcgaagcc ttgggtttgg ccgatgcggc cggcagcttg
    11461 ggaggtggtg cgggccaggt gagctcaccc tgcttgatca cggtgaggcc acggatcgcg
    11521 tcgtcctcga agttgacggc gatgttgcca tcctttgcct tgcacaggtc ttcggtgagg
    11581 cggaggaggt tgttcgaata cagggtcgag gattgcctgg ccagccggct cacgaggtcg
    11641 gtgtagccga tgatcgtgac tccatggcgt accaccgcct cgccgggcac ggtgagctcg
    11701 cagttgccgc cctgctcggc ggcgaggtcg acgatcacgc tgcccggctt catgctctgc
    11761 accatctcgg cggtgatcag cttgggtgcg ggtttgcccg ggatcagcgc ggtggtgatc
    11821 acgatgtcgg cgtccttcgc ctcgcgcgcg tacatctggc gctgcgcggc ctggaagccc
    11881 tcgctcatca ccttcgcgta accgccgcca cccgagccct cttcgtcgta atcgaccttg
    11941 acgaactcgc cgccgagcga caccacctgg tcggcgacct cggcgcgggt gtcgttggcg
    12001 cgcacgaccg cgcccaggtt ggccgcggtg ccgatcgccg ccaggccggc gacgccggcg
    12061 ccggcgatga agaccttggc cggcgggatc ttgcccgcgg cggtgatctg gccgttgaag
    12121 aagcgcccga aggcgttcgc cgcctcgacc accgcgcggt agccgctgat gccggccatg
    12181 gaagacagcg cgtccatctt ctgcgcgcgg ctcagctgcc gcggcaggca gtcgatcgcg
    12241 agcacggtgg tcttgcgcgc ctgcaaggcc gccatcagtt cggggtgctg cgccggccag
    12301 acgaagccga tcagcgtcgc gccctcgcgc agcatgccga cctcatccag cgtgggcggg
    12361 cgcaccttga agacgatgtc ggcacggccc agcagctcgg ccgacccggg cgacacctcc
    12421 gcgccggcag cgcggtagtc gtcgtcggag aagttggccg ccgcgccggc gccgctctcg
    12481 accgcgacgg agaagcccag cttgatgagt ttcgccacga cctcgggaac ggtcgcgacg
    12541 cgcttttcac cggcgaaggt ttccttcgga actccgatca gcaaggtcat gatcccgact
    12601 ccgcgcaggt ttgattgtgg aagcggggcg cggccgtgtg gctggtgcgg gcatgcccgg
    12661 ggaagtcttg cgccgtcgct gcgcgcgggc tccccggggt gggtgcgaag cggatttcac
    12721 gaagcgtggc tccgcaagcc atggtcgggt gcatcgggtt ctccctgttc ggttttcgtt
    12781 ggtgccggcg tgcgtattcg tctcggacat gcccgcctgg cccgggcgaa ctcatgggcg
    12841 atcgcattcg cgtcgattgc cgatgttcgt tcccttggcg agtatgccgg atccgacctg
    12901 gctttcgata gccagggcga tgcccgggcc accgccgatg caccgtgaag cgaggccgcg
    12961 tcccggcgtg agcatgcgtg catccgcgcc cggcaccgcg tggcgctcga agcagatcag
    13021 gtcgccatcg tgcgctgccg agggcgggtt cttgatggtc tgcttggcca caccaaaaaa
    13081 ccttatcctg cccgcctgaa gttctatcga gctgcaaggg ctatccatcg cacgaggctg
    13141 cggtggtcag ggacgtggaa tgtgcggcgt cccccgcgcc acgcgaacct gccggagcag
    13201 cgcggaaacg tcggaatcca aggagatcgt catcatgtcg atcggcgcat cattcagcgc
    13261 agtcgtgcag ccgaggcggg cgcgccggtc ggataccatt gccgagcaga tcgagggctt
    13321 gagcacgggc ggagcctcgt cgtccgtgcg gcggtcccga tcgcgcggcc gtctgccccc
    13381 gttggcacgc tgcgaagcct cgaggtcacg atttcgagca tgaaccccaa gccccgccac
    13441 atcgaacgcg cgctccggga gcgcgtcgag atcgtgccct acgatccggc ctggccgggg
    13501 ctcttcgccg aggaggtggc gcggctgcgt gcggccctgc cggcggagct gatcgggcgc
    13561 atcgagcact tcggcagcac cgcggtgccc ggcctggcgg ccaagccgat catcgacatg
    13621 ctggtcgagg tacgctcgat ggaggacgta gcggagcgca tcgcaccgct gctgcgggcg
    13681 ctgggctacg agttcttctg gcgcgccccc gggcacgggc tgcccggcat ggagtacgcg
    13741 tggttcatcc gccgcgatgc tgcgggccgg cgcacgcatc acatccattt tgtcgaggcg
    13801 ggctcgtcgg agtgggggcg cctgcggttc cgcgactacc tgcgcgcgca ttccgaagtc
    13861 gcgcgagcgt atggcgcact caagcagcgc attgcggacc aatatcccga cgaccgcatc
    13921 gcctacgcga aggcgaagac gtgcttcgtg cgcgaggtga tgcgggcggt gcgacgggat
    13981 cacatccctt ggtagatcgg cccttcgcca ccctgcggca cgacccagtt gatgttctgc
    14041 gtcgggtcct tgatgtcgca ggtcttgcag tggatacagt tctgcgcgtt gatctgcagg
    14101 cgcggcccgg attcttcgcg gacgatctcg tacacgccgg cggggcaata gcgctgctcg
    14161 ggagcgtcgt acttggccag gttcaccgcg atcgccaccg acggatcctt cagctgcagg
    14221 tggcagggct gctcttcctc gtggttggtg ttcgagagga agaccgagga caggcggtcg
    14281 aaggtgagta cgccgtcggg cttcggatag tcgatcttcg ggcactcgga agccggtttg
    14341 agcttgtcgt ggtccgacga gttgtgcagc gtccaggggg ccttgccgcg gaacagcatc
    14401 tggtccatgc cgaagaggaa ggagcccatc cacaggccct tcttcatcag cggcttgaag
    14461 ttgcggtatt tgtgcagttc ggcgtacagc caggagtcgc ggaaggcggc cgggtaggcg
    14521 gtgagcgcgt cgcgctggcg attcgcggcg agcgcgtcga aggccgcttc ggcggcgagc
    14581 atgcccgact tgatcgcggt gtgcgtgccc ttgatgcggg cggcgttgag gaagccggcg
    14641 tcgtcgccga tcaggccgcc gcccgggaag atcagccgcg gcaggctctg gatgttgccg
    14701 gtggcgatgg cgcgcgcgcc gtaggccagg cgcttgccgc cttcgatgtg cttgcgtacc
    14761 tcggggtggg tcttccagcg ctgcatctcg tcgaagggcg acaggtgcgg gttctcgtag
    14821 ttgaggccga ccacgaagcc gagcgcgacc aggttgtcct cgaggtggta gatgaagccg
    14881 ccgccatagg tgcggttgtc catcggccag ccggcggtgt gcacgacgag gcccgggcgg
    14941 tgctgctcgg gcttgacctc ccacagctcc ttgatgccga tgccgtaggt ctgcgggtcg
    15001 acgccctcgc gcagcttgta cttcgcctcc agctgcttgc cgaggtggcc gcgacagccc
    15061 tcggcgaaca gggtgtactt ggcgtgcagt tccatgcccg gctggtggtg cgggccgggg
    15121 gtgccgtcac ggttgaggcc catgtcgccg gtggcgacgc ccttcaccgc gccggattcg
    15181 tcgtagagga tctcggccgc ggcgaagccg gggtagacct cgatgccggc ggcctcggcc
    15241 tgctcgccca gccacttgac gaggttgccc aggcgcacga tgtagttgcc gtggttctcg
    15301 aagcagtccg gggtgagtgc gggcggaacg cggcgcacgc cggtctcgga aaggaacagc
    15361 acctggtcct cggtgaccgc ggtggtgagc ggggcgccct tgtccttcca gtcggggatc
    15421 agctcgttca gcgagcgcgg atcgatgacc gcccccgaca ggatgtgggc gccgatctcc
    15481 gcgcccttct cgatcaggca caccgacagc tcgctgccct tggcttcggc gagctgcttc
    15541 aggcggatcg ccgcggccag gcccgcaggg ccgccgccga cgaccagcac gtcgaattcc
    15601 atcgattcgc gttccatacc gttctcctcg tatcttcttt cagtgggtcg cgtagcgaga
    15661 cgcgccatcc gcgcagttta acccttcgtg cgaatccccg ccgccggtgg gcgcgcgcat
    15721 gcgcgcgccc accggcggcg gggcgaacaa acatacgggt gcgtatgtta cattagcccg
    15781 ccccatatac aagaggagat cgtcggatga gtccgaacgc gaagctgatc tacaccaccc
    15841 gcatgccggt gcgctggggg gacatggacg cctacgggca tgtcaacaac acggtctatt
    15901 tccgctattt cgagcagacc cgcgtcgagt ggctcgaacg gatgggttcc cgcgtcagcg
    15961 tcgaggagcc ggtggggccg gtgatcatca atgccagctg caccttcctc gcgccggtga
    16021 attatcctgc gaccgtcgtc atcaagatgt atgcaggcga tccggggcgg agcagcgtga
    16081 tgacctggta cgagctcttc gtcgatggcg acgagcgcct gtacgccgaa ggtgcggcca
    16141 agacggtgtg gatggacatg cgcaccggca agtcggcgcc gattccggat gtcgtgcgcg
    16201 cgctgttcga cccgcccgtg gcctgaattg gcgccggccg ccgagtgccc ggccgcgcag
    16261 tgcggtcacc cgctccgtcc gcgaagcgtg gccgcccagt ttcgaccccc agagttttga
    16321 cccccaatca tcgacacaag aggatggaga ggagacccga tgtatccgac tgagcagaaa
    16381 cccctgtgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac acaagaggat
    16501 ggagaggaga cccgatgtat ccgactgagc agaaacccct gtggaccccg tcccccgagc
    16561 gcatcgccgc cgccaacgtg actgccttcc ggctcgcggc cgagcagcgc tggaaggtct
    16621 cgctgcccga ttacgaggcg ctgcacgcct ggtcggtgga gcaccccgag ccgttctgga
    16681 cgagcgtttg ggagggcgac ggcgtcggtg gcggcgtgat cggcacgcgc ggcgagcgcg
    16741 tgctcgtcga tggccacaag atgcccggcg cgcgctggtt ccccgacgcc cgcctgaact
    16801 tcgcgcagaa cctgctgcgt tcgcgcgacg cgcatgacgc gatcgtgttc tggggcgagg
    16861 agcgcgtgaa gaaccgcctg agccacggcg agctctaccg cgcggtggcg cactttgccg
    16921 cggcgctcaa ggagcagggc gtggtggccg gcgaccgcgt cgccgcgtac atgcccaacc
    16981 tgcccgagac cgtcgtcgcc atgctcgccg cggccagcct cggcgccatc ttcacctcgg
    17041 cctcgcccga cttcggcgtg cagggcgtgc tcgaccgctt cggccagacc gagcccaagg
    17101 tgctggtggc ctgcgacggc tactactacg gcggcaagac ggtggatgtg cttggcaagc
    17161 tcggcgagat cgtcgcgcag ctgccctcgg tcaagcgcgt cgtggtcgtg ccctacgtgc
    17221 atcgcgagca cgacctcttg cacgtgccgc acgcgcgcat gctcgccgac ttcgtcgcgc
    17281 ccttccactt cgtcgacgac atcgagttcg cgcagctgcc cttcgaccac ccgctctaca
    17341 tcatgtactc ctcgggcacc accggcgtgc ccaagtgcat cgtgcactgc gccggcggcg
    17401 cgctgctgca gcacctgaag gaacacaagc tgcacggcga cgtgaagccg ggagaccgcg
    17461 tgttctactt caccacctgc ggctggatga tgtggaactg gctggtctcc gcgctcgccg
    17521 ccgaggccac gctgctgctc tacgacggct cgcccttcgc cggcgacaac cgcatcctct
    17581 tcgactacgc cgacgccgaa gcgatgacgc acttcggcac ctcggccaag ttcctcgacg
    17641 ccgccgccaa gttcgggctc aagccgcggg agacccacaa gctcgacacg gtgcgcgcga
    17701 tgatgagcac cggcagcccg ctggtgcccg agggcttcga ctacgtctat cgcgacatca
    17761 aggccgacct gcagctctcg tcgatctcgg gcggcaccga catcatctcc tgcttcgtgc
    17821 tcggcagccc ggtgctgccg gtgtggcgcg gcgagatcca gtgccgcggc ctgggcatgg
    17881 cggtggacgt gtgggacgac gacggccgcc cggtgcgcgg cgagaagggc gagctggtgt
    17941 gcgcgaagcc cttcccggtg atgccgatcg ggttctggaa cgacgccgac ggcagcaagt
    18001 accacgccgc ctacttcgat cgctttccca atgtctggtg ccacggcgac ttctgcgaga
    18061 tcaccgcgca cggcggcctg gtcatctacg gcaggtcgga cgccacgctg aacccgggcg
    18121 gggtgcgcat cggcaccgcc gagatctacc gccaggtcga gaagctgcac gaggtggtcg
    18181 agtcgctcgt gatcggccag gactggccgc cgcagaaccc caacgacgtg cgcgtggtgc
    18241 tgttcgtgaa gctgcgcgag gggctcgcgc tcgatgacga cctgaccagg cgcatccgcc
    18301 agaccatccg cgacaacacc acgccgcgtc acgtgccggc caaggtgctg caggtggccg
    18361 acatcccgcg caccaagagc ggcaagatcg tcgaactggc ggtgcgcaac gtggtgcacg
    18421 ggcgcccggt gaagaaccag gaggcgctgg ccaaccccga ggcgctggcg cacttccgcg
    18481 accgcgccga gctgcagggc tgaggaggat cgaagacatg ctgatgaacc cgaagaagtc
    18541 ggtgctgctg gtgatcgacg tgcaggcgaa gctcgccccc ttcatccacg acaacgccgc
    18601 ggtggtcgag gcctgcgtgt ggcttgcgcg cgtcgccgag cgcatcggcg tgccggtggt
    18661 ggtcaccgag cacttccccg agaagatcgg cggcacggtc gaggcggtgc gcgcggcgac
    18721 cgccggtgcg cactacgtcg gcaagcagtt cttctccgcc caggccgatg gctgcctcga
    18781 ccagaccgcg gtcgccggct gcgagcaggt gatcctgtgc ggcaccgagg cccatgtctg
    18841 cgtgcagcag accgcgctcg acctgcgcgg cgccggcaag caggtcttca tcgtcgccga
    18901 ggcctcgggc tcgcgcgctc cggccaaccg cgacctcgcc ttcgagcgca tgcgcggcca
    18961 cggcatcgag atcgtgtcgc gcgagatggt cgccttcgag tggctgcagc gcggcggcac
    19021 cgagctcttc cgcgaagtca atcgcgactt catccgctga ggatggccgg caaggccggc
    19081 cctgcaggat caccccgtcc ggcccctggc gcgtgcgcgt cccgaactag ggaaaacgtg
    19141 cgtatccatg ccggacgggt tgttctagcc tgaccgcttc gttccacggc gatgcgatgt
    19201 gcgctccctc gacgctgtcc ataccgagct cgcagaccgt cccgccgtat ccaaaagcgc
    19261 aagcgggagg ttggagatgg agacacggga agcaggccgg ctggccacgc aggtcgaggc
    19321 ggtgatcgag cagattcgca cccggatgcc cgccgagcag gccgaagggg tgtgtgcgtt
    19381 cgcgagccgc tttttcgccc aggtggcgcc cgaggacctg gaagacctcc cggtgggcga
    19441 tctctacggc gccgtgctca gccagtggca cttcgtcgcc cgccgccagg acggcagcaa
    19501 ggtgcgcgca ttcaacccgc ggctggacga gcacggctgg gagtgcccgc acaccgtggt
    19561 ggagatcgcc ggcgaggaca tgcccttcct cgtcgactcg atcaccatgg aggtcgcgcg
    19621 ccagggtctc accctgcacc tgatcatcca tccggtgatg aacgtcgcgc gtgatgccga
    19681 gggcggcttc gtgcgtgtgg cggagcgcgg cgagcagggc gagggtgtcg gcttcgagtc
    19741 gatcatgcac ctggaggtcg accgccgcac cgacccgggc gacctcgttg cgctgcagca
    19801 aggcatcgag catgtgcttg ccgacgtgcg tgccgcggtc cgcgactggt cggccatgcg
    19861 cgagcgcctg gccggcatca ccgcagggct cgacgagctg ccggtcagca tcgacgccga
    19921 ggagacgcag gaggtgcgcg ccttcctcga ctggctcgcc gccgacaact tcgtgctgct
    19981 cggctgccgc gactacgcgc tggtcgagac cggcgagggc aacgagctgc gcatcgtccc
    20041 cggctccgga ctgggcctgc tgcgcggcga cggtgacgag gggcagtcgc gctcctttgc
    20101 tgcgctgccg ccgcagctgc gcgcgcaggc ccacctgccg catctgctca cggtcacaaa
    20161 gtcgaactcg cgctccacgg tgcaccggcc cggctatctc gacttcgtcg gcatcaagac
    20221 cttcggggcc gacggccggg tgaatggcga gcgtcgcgtg atcggcctgc tcgcctccac
    20281 cgcctacagc gccagcccgc gccagatccc gctgctgcgg cgcaaggtgg aggccgtgtt
    20341 cgagcgtgcc ggcctgctgc ccggcggcca cgccgccaag gcgctgcaga ccctgctcga
    20401 gcgctacccg cgcgacgagc tgttccagat cgagaccgac gagctccacg cccacgccat
    20461 gggcatcctg cgcctcggcg agcgcctgcg cacgcgcctc ttcgtgcgcg tcgatccctt
    20521 cgagcgcttc gtgtcgtgcc tgatctacgt gccgcgcgag cactacaaca ccgaccagcg
    20581 cgagcgcatg caggcggtgc tgatcgatgc cttcaagggc aacgccgccg agttcgacgt
    20641 gcagttttcg gattcggcgc tcgcgcgcat cctcatcacg gtgcgcacgc ccgagggtcg
    20701 gatccctgcc ttcgacgtgc gcgagatcga gcagcgcctg atccgtgcgg cgcggcgctg
    20761 ggaggacgag ctgcagcagg cgctcgtcga gcagtgcggc gaggagcgcg gcctggcgct
    20821 gatgcggcgc tacggcgagg gcttcccggc cggctaccgc gaggaatact ccgcgcgcat
    20881 ggcggtgttc gacatcgagc agatggaggc gctcgccgac gacgcggcgc tcgggctcaa
    20941 cctctacgtg ccgctcgagg tgcgcgcggg ccggctcaac ctgcggctct accacctggg
    21001 cagcccggta ccgctgtcgc agagcctgcc gatgctcgag aagatgggcg tgaaggtgat
    21061 ggacgagcgc ccctccgaga tcgagcgcca ggacggcagc acggtgtggc tgcacgactt
    21121 cggcctgcag tttgccggtg ccgagaacct caacatccac gacatccgcc cgctctttca
    21181 ggaggccttc ctcgcggcct ggcgcggcgc cgccgagaac gacgatttca accgcctggt
    21241 gctgctcgcc gggctgtcgt ggcgtgaggt ggcggtgctg cgcgcctacg cccgccacat
    21301 gcgccaggcg gcgttcacct tcagcctcgc ctacatggag cagacgctgg cggcctatcc
    21361 acagttcgcg cgtgcgctgc tgcaactctt ccgcgcgcgc ttcgacccgg cgctcgcggg
    21421 cgatcgcgag gccgcgtgcg cgacgcaggt ggcggccatc gaggccgcgc tcgatcaggt
    21481 ggccaacctc gacgaggacc gcatcctgcg ccagtttctc gccctcatgc aggcgaccct
    21541 gcgcaccaac tggttccagc gcggcgcgga cggcgccccc aagccctacc tctcgttcaa
    21601 gttcctgccg tcgaagatcc ccaatctgcc gcagccgctg ccgatgttcg agatcttcgt
    21661 gtactcgccg cgcttcgagg gcgtgcacct gcgcggcggc aaggtcgcgc gcggcggcct
    21721 gcgctggtcg gaccgcatgg aggacttccg caccgagatc ctcggcctgg tgaaggcgca
    21781 gatcgtcaag aacgcggtga tcgtgccggt gggctccaag ggcggcttcg tggtcaagtg
    21841 cccgcctgcg gagggcgggc gcgaggccct gctcgccgag ggcgtggcct gctaccgcaa
    21901 cttcctgcgc ggcctgctcg accttaccga caacctggtg cagggcgcgg tggtgccgcc
    21961 ggcggacgtg gtgcgccacg acgaggacga cccctacctg gtggtggcag ccgacaaggg
    22021 cacggcgagc ttctccgact acgccaacca ggtctcggcc gagtatggct tctggctggg
    22081 cgacgccttc gcctcgggcg gctcggtggg ctacgaccac aagaagatgg gcatcaccgc
    22141 acgcggcgcc tgggaggcgg tcaagcgcca cttccgcgag atgggcaagg acatccagca
    22201 ggaacccttc accgtggtcg gcatcggcga catgtcgggc gacgtgttcg gcaacggcat
    22261 gctgctgtcg aaacagatcc gcctggtggc ggccttcgac caccgccaca tcttcatcga
    22321 ccccgatccc gacccggcac gctcgtggga agagcgcgcg cgcatgttcg cgctgccgcg
    22381 ctcgagctgg gacgactacg accgcgcgct gatctcgcag ggtggcggag tgtggccgcg
    22441 cagcgcgaag tccatcacgc tgtcgcccga ggcgcgcgcg gcactcgaca tccaggccgc
    22501 gcccctgggc ttgaccccga ccgagttgat ccgcgccatc ctcaccgcac cggtcgagct
    22561 ggtctacaac ggcggcatcg gcacctacat caaggccgcc agccagaccg acgcggcggt
    22621 gggcgaccgc gccaatgacg cggtgcgcgt caatggcggc gaactgcgct gcaaggtctt
    22681 cggcgagggc ggcaacctcg gcgccaccca gctcggccgc atcgagttcg cgctcgcggg
    22741 cgggcgcatc aacaccgacg cgatcgacaa ctccggcggg gtggactgtt ccgaccacga
    22801 ggtcaacatc aagatcctgc tcggcggggt gatcgccgag ggcgagctca cgctcaagca
    22861 gcgcaacgag ctgctcgccg acatgaccga cgaggtcggc atcctggtgc tgcgcgacaa
    22921 ctacgcccag acccaggtgc tgtcggtcac gcgcgcgcgc ggcgtggcgc tgctcgacga
    22981 gcaggcggag ttcatccgcc gcctggtgca ggccgggcgg ctcaaccgca agctcgagta
    23041 cctgcccgac gacgaggaga tcgccgagcg caaggccgcc ggcgtcggcc tcaccagccc
    23101 cgagctggcg gtgctgctcg cctacagcaa gatcgagctc tacgacgagg tgctcgcctc
    23161 cgacgtgccg gaggacccct acatccgcac cgcgctcgag cgctactttc ccggcccgct
    23221 gcgcgagcgc ttcccggcgc agatccggat ccacccgctg cgccgcgaga tcatctcgac
    23281 ccacgtggtc aacagcatga tcaaccgcgt cgggcccacc ttcgtcagcc ggctcagggc
    23341 cgagctcggc gcctcggcgg cggacgtggt gcgcgcctac atggccaccc gcgaggtctt
    23401 cggcctggtg gcgctgtggc gcgagatcga ggccctcgac aaccgcatcg ccgacgcggt
    23461 gcagaccgag ctcatccagg agtcgggccg cctggtgcag cgcggcacgc tgtggttcct
    23521 gcgtcaccgc cgctggctgg ccgacctgca ggcgacgagc gcgcatttct cgcctggcgt
    23581 ggcggcgctc gccgaggggc tggccgacta cgtcgccccc gcctatcgcg ccgagctcga
    23641 cgccgcggtg gcgcgccggg tggagcaggg cgtgcccgag gcgctggcga agcgggtggc
    23701 cgcgctcgaa gagctttact cggcgctcga cctggtcgag gtggcggtgg agcaggggcg
    23761 tgacgaggcc acggtggcgc gggtgtattt cgccctcggg ggcgaattcg acctgcactg
    23821 gctcggccgc cagatctccg ggctgcccgc ggacacgcgc tggcagagcc tggcgcgcgg
    23881 cgcgctgcgc agcgatctgt cctcgctcgc ccgcgagctc accagcgcgg cgctgcgcaa
    23941 cgcgcccgag ggcgcggaca ccgagggcgt gctgaatgcc gggcgcgagc gtgccgcggt
    24001 gccgctcgag cgctaccagc agctcctcgc cgaaatccgc agcgcgccga cgatcgacat
    24061 ggcgatgctg tcggtgctgc tgcgcgagct gcgcggcatg gcctgagcga tcgcgcggga
    24121 ggcacccgga ctgctgacgc ggttcgggtg catcggttat catcgcgctt tcccgcagta
    24181 cagctcccat gaccaaatac gtcttcgtca ccggtggtgt cgtgtcctcc ctgggcaagg
    24241 gcatcgccgc agcgtccctc ggggcgatcc tcgagtcccg cggcatcaag gtcacgcacc
    24301 tcaagctgga cccctacatc aacgtcgatc cgggcaccat gagcccgttc cagcacggcg
    24361 aggtcttcgt caccgaagac ggcgccgaga ccgacctcga cctcggccat tacgagcgct
    24421 tcaccagcgc caagatgagc aagcgcaaca acttcaccac cggccagatc tacgagtcgg
    24481 tgctgaagaa ggagcgccgc ggcgagtacc tgggcaagac cgtgcaggtc atcccgcaca
    24541 tcaccgacga gatcaaggct tacgtcaagc gtggcgccga gggcgccgac gtggcgatcg
    24601 tcgaggtcgg cggcacggtg ggcgacatcg aatcgctgcc cttcctggag gccatccgcc
    24661 agatgggctt cgaagagggc cgccacggca cctgcttcat ccacctcacg ctgctgccct
    24721 acattccgac cgccggtgaa ctgaagacca agccgaccca gcactcggtg aaggagctgc
    24781 gcgagatcgg catccagccc gacatcctgc tgtgccgcgc cgaccgtccg g
//
LOCUS       DAOQBS010000035        24386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1311_length_24386_cov_15.416300, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000035 DAOQBS010000000
VERSION     DAOQBS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 24386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24386
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1311_length_24386_cov_15.416300"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..615)
                     /locus_tag="PKN25_05985"
     CDS             complement(115..615)
                     /locus_tag="PKN25_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV90484.1"
                     /translation="MPSLAWSADLDNNLAPMDETHREFVECYNAAANAAPDDFLRALD
                     ALIEHAKAHFDLENGWMEAVDFPGCHRAEHDRVLAVMADIRKRVERGDHFLGRRLMEE
                     LPAWFVNHVNGMDAALAFHLATIGFDVDSCTLRPLAEGETRAAGCGCATLAAEDAAAG
                     EAARAA"
     gene            complement(625..1095)
                     /locus_tag="PKN25_05990"
     CDS             complement(625..1095)
                     /locus_tag="PKN25_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HNV90485.1"
                     /translation="MSTVSTKPYLLRAIWEWCVDQGFTPYLATLVDEDTRVPPGYARD
                     GQIVLNLSPDATGQLQMANDFISFQARFGGAAHSLVIPVANVVAIYARENGQGMAFEP
                     ELEAVEDASVAVQDPSADVVQDMSDTTAELRRERSAEPQPPARPPRGSHLKIVK"
     gene            complement(1160..1756)
                     /locus_tag="PKN25_05995"
     CDS             complement(1160..1756)
                     /locus_tag="PKN25_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNV90486.1"
                     /translation="MMNLYSGTTDPFSHRCRIVLFEKGMDFEVIDVDLYNKPEDIAVI
                     NPYNRVPVLVDRDLVLYEANIINEYIDERFPHPQLMPPDPIMRARARQLLHTFEQELF
                     THLEALEAAQKGVDKVRAHVRDHLTQLAPVFTKQKYMLGEEFSMLDVAIAPLLWRLEH
                     YGIELPKAAAPLMKYAERIFSRQGFIDALTPSEKVMRR"
     gene            complement(1864..2619)
                     /locus_tag="PKN25_06000"
     CDS             complement(1864..2619)
                     /locus_tag="PKN25_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HNV90487.1"
                     /translation="MKTRVINFIKRFAAVALFAPAVAMAAGASVHLDKAPVSSEPAAL
                     QHGAKLFVNYCMNCHSASFVRYNQLQNIGLSEAEIRDNLMFTGERVGDLMKIAMRPAE
                     SKGWFGVAPPDLTLVARQRASADGSGADWLYTYLRQFYRDPSRPTGWNNAIFANVAMP
                     HVFWQLQGEQVAKITQNADGTTTTALELVKPGKLSVQEYDKAVADLVSFLVWMGEPVA
                     EKRKSIGALVLIFLAGMFVLSYALKKNYWKDIH"
     gene            complement(2616..3884)
                     /locus_tag="PKN25_06005"
     CDS             complement(2616..3884)
                     /locus_tag="PKN25_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNV90488.1"
                     /translation="MTTKSQAVLNWIDERFPLTSTWKAHLSEYYAPKNFNFWYFFGSL
                     ALLVLVIQILTGIFLVMHYKPDASLNASGVPVAFASVEYIMREVPGGWLIRYLHSTGA
                     SAFFIVVYLHMFRGLLYGSYRKPRELIWVFGTLIFLVLMAEAFMGYLLPWGQMSFWGA
                     QVIVNLFSAIPVIGPDLSLVIRGDYVVSDATLNRFFSFHVIAVPLVLIGLVLAHIVAL
                     HEVGSNNPDGVEIKKKKDATGKPLDGIPFHPYYTVKDIVGVVVFLFFFSAVVFFMPEG
                     GGYFLEFNNFIPADPLKTPPHIAPVWYFTPFYSILRAVTYPLFGLDAKFWGVVAMGAS
                     VVIIAFLPWLDRSPVKSIRYKGPIFKMVLVVFVIAFFILGYLGVLPPTPGRTLVSQIC
                     SVIYFAFFLLMPWYSKLDKCKPEPERVNFK"
     gene            complement(3888..4481)
                     /gene="petA"
                     /locus_tag="PKN25_06010"
     CDS             complement(3888..4481)
                     /gene="petA"
                     /locus_tag="PKN25_06010"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNV90489.1"
                     /translation="MSVDQKMDSSRRTLLLATSAAGGVAAVATAVPFVASLTPSERAK
                     AAGAPVEADVSKLKPGEMMTVEWRGKPVWILRRTPEMLASLDKTDEKVSDPNSVKPQQ
                     PEYAKNKHRSIKPEYLVTVGICTHLGCSPSEKFKVGADSGMGADWPGGFLCPCHGSIF
                     DLAGRVYSSMPAPDNLEVPPHQYLADTKILVGDDGKA"
     gene            complement(4667..5260)
                     /gene="recR"
                     /locus_tag="PKN25_06015"
     CDS             complement(4667..5260)
                     /gene="recR"
                     /locus_tag="PKN25_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNV90490.1"
                     /translation="MSPSSLDELIEALRCLPGVGPKSAQRMAYHLLQRDQRGAARLGN
                     ALLHALEVLRHCARCNSFTEDEVCPRCANPRRDAALLCVVEMPADLAMIEQTQSYNGL
                     YYVLMGRLSPLDGVGPRELRLDKLIARATEEGVREVILATNFTNEGEATAHTVASLLG
                     ARGVKVSRLSRGVPVGGELEHTDIGTIGQALIERRAV"
     gene            complement(5272..5595)
                     /locus_tag="PKN25_06020"
     CDS             complement(5272..5595)
                     /locus_tag="PKN25_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNV90491.1"
                     /translation="MMKGGIAGLMKQAQQMQENMKKMQDQLGSIEVEGQSGAGMVKVQ
                     MTCKYDVRRVSIDESVMDDREMLEDLVAAALNDAVRKVEAATQEKMSSVTAGLNLPPG
                     MKLPF"
     gene            complement(5669..7819)
                     /gene="dnaX"
                     /locus_tag="PKN25_06025"
     CDS             complement(5669..7819)
                     /gene="dnaX"
                     /locus_tag="PKN25_06025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNV90492.1"
                     /translation="MSYQVLARKWRPKSFGTLVGQEHVVRALTHALATGRLHHAWLFT
                     GTRGVGKTTISRILAKALNCETGITAEPCGICDACRAIDADRFPDYVEMDAASNRGVE
                     EMAALLDKAVYAPVQGRFKVYMIDEVHMLTGHAFNAMLKTLEEPPEHVKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMPQGHIVDHLTRILQAEEVAFEPGALRHLAKAASGSMRDAL
                     SLLDQAIAHGAGRVEEEQVSHMLGTVGDDHLYAVLDALAAGDVPALLAVADGMEARSL
                     SFDAALQALATLVHRVALAQYAPAAIADELERARILGYVERFDAEFLQLAYQVAVHGR
                     DELPLAPDDYTGFTMTLLRLHAFRPEQPAALGGPGVPSGPDAGRARAPALAAGGPAES
                     GRAAESGVPAEVPARTVASAGIAGPAPAAVPAPAAGADAAIPSAQQTVLEAAAPASMQ
                     PGSVPAAQQAVAPAPASAPPRRSAREEHAVQPARAAPASVQDVPPWEDLPPEAYADSD
                     LPPPREARNGHDEAYAASAGAHTGAISRARPEPRGGGARRAEEVPSRSAPLPAVRAPE
                     PATSPAAPTQDAPAASGDAAALLALGDWRGLIRALGLGGLVRELAQHCEWVECGDGVL
                     QLRLSTAHRHLLDMNRGAVERLQDVLCAALGRTLAVRIAIGDISGQTPAQRDAIERQV
                     RHAEAVAALEADPFVRELIERFDATLVENTVKPL"
     gene            complement(7878..9797)
                     /gene="cysN"
                     /locus_tag="PKN25_06030"
     CDS             complement(7878..9797)
                     /gene="cysN"
                     /locus_tag="PKN25_06030"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNV90493.1"
                     /translation="MAHVSDLIATDIEQYLRAHENKSLLRFITCGSVDDGKSTLIGRL
                     LFDAKMLFEDQLAAIEADSKKWGTQGGDIDFALLVDGLAAEREQGITIDVAYRFFSTD
                     KRKFIVADTPGHEQYTRNMVTGASTADAAILMVDARKGILTQTRRHSYLVNLIGIRHI
                     VVAINKMDLVDYAEGVFRRIVEDYTAFSRQLGIEQVTFIPMSAFRGDNITSPSAAMPW
                     YHGTTLMGYLETVEVDDGLMQRAPFRLPVQWVNRPNLDFRGFAGSIAGGTIRPGDRVR
                     VQPSGRESTVARIVTRDGDLDRAVAGQSVTLTLADEIDISRGDVISTVEAPAEVADQF
                     ECTVVWMHDEPMLAGRPYLLKIGARTVSATITEIKYQVNVNTLEHVAAKRLELNAIGV
                     CNLSLDRPIAFDPYRINRDTGGFILIDRLSNNTVGAGLLHFALRRAHNIHLQHVDVDK
                     RARAALKNQRGCVLWFTGLSGAGKSSIANLVEKKLHALGHHTYLLDGDNVRHGLNKDL
                     GFTDADRVENIRRVAEVAKLMVDAGLIVLTAFISPFRSERRMARGLVEEGEFVEVFVD
                     TPLEVAEARDPKGLYKKARRGELKNFTGIDSPYEAPEDPELRLDTTRLDLEAAADAVL
                     AWLGERGMLSRTPGG"
     gene            complement(9798..10715)
                     /gene="cysD"
                     /locus_tag="PKN25_06035"
     CDS             complement(9798..10715)
                     /gene="cysD"
                     /locus_tag="PKN25_06035"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNV90494.1"
                     /translation="MTSVPTTSLTHLQRLEAESIHILREVVAEADNPVMLYSIGKDSA
                     VMLHLALKAFHPAVPPFPLLHVDTRWKFRDMYSFRDRMAGETGMDLLVHINPEGVEKD
                     INPFTHGSAIHTDIMKTEGLKQALDQYGFDVAFGGARRDEEKSRAKERVFSFRTAQHR
                     WDPKNQRPELWKLYNARKHKGESIRVFPLSNWTELDIWQYIYLENIPIVPLYYSAVRP
                     VVERDGTLIMVDDERMPLRPGEVPMMKKVRFRTLGCYPLTGAIESEADTLPAIIQEML
                     LARTSERQGRVIDHDSAASMEKKKQEGYF"
     gene            10910..11680
                     /gene="cysQ"
                     /locus_tag="PKN25_06040"
     CDS             10910..11680
                     /gene="cysQ"
                     /locus_tag="PKN25_06040"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNV90495.1"
                     /translation="MDTATASRLLEALLPIVRAAGALVMDIYRSDFAVRGKDDASPVT
                     EADERAEALIVPALEALLPGVPVVAEEAVAAGRLPALGRRFWLVDPLDGTKEFIGRNG
                     EFTVNIALVEDGEPVLGTVFAPALERLFLGAGGVGAFVEQDGRRRPIRCRTVPPAGLT
                     VVASRSHGDAAALDAFLDGRKVAALTNAGSSLKLCLVAAGEADLYPRLGRTMEWDIAA
                     GHAVLAAAGGRVQTLAGAPLRYGKPGLDNPHFVASGLD"
     gene            11695..13431
                     /locus_tag="PKN25_06045"
     CDS             11695..13431
                     /locus_tag="PKN25_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNV90496.1"
                     /translation="MTTAHLVLASIALLLALLAHGRVGPAVLFAAWAGGYHLLGLASQ
                     QELLASFSNPALATLILLLLVSLALERSPLLDHLSELLLKGRVGLATLRLSVFACLIS
                     AFMNNTAVVGALLGTVTSQRRHPASKLLIPLSYASILGGITTLVGTSTNLVVNSFVID
                     AGLPPLQMFQFAWVGIPVALACIGVMALSSRLLPANASSEAEARSTYFLEARVAAGSP
                     MIGRSIETNRLRNLEGLFLLEIEREGRLISPVGPAEILHTGDVLVFTGEVARVQTLQR
                     FPGLEVFGAGADALLKSNLVEVVLASSSDLANRTLREVDFRTMFDAGVVGIRRGDRRL
                     TGQLGRIPLRVGDSLLLAAGPDFLQHRNLDRNFHILNGSGLRPKLGRMQSVAALSGFA
                     LVILVAALGWLPLFGGLLVLLAALLGSGLLTLGELRRRFPFELLVVIGSALAIAGVVE
                     RSGAAELVAGWMRAIFDGHGVHAALAGVYLITLTLTEVITNTAAAALAFPIALGTARA
                     FGVDPMPFVMVVAYGASAGFLIPFGYQTHLMVYSAGRYRMRDFLRAGLPVSLTYSALV
                     LLLVPLVFPLHR"
     gene            complement(13447..13713)
                     /locus_tag="PKN25_06050"
     CDS             complement(13447..13713)
                     /locus_tag="PKN25_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNV90497.1"
                     /translation="MSEQILKEVLSLLDEVLSLDGRALAFAADTPLLGDLPELDSMAV
                     IAVIQEIEARFGIEVPDDEVDGNSFATVGTVVEFVTRLSARQPG"
     gene            13825..15417
                     /locus_tag="PKN25_06055"
     CDS             13825..15417
                     /locus_tag="PKN25_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HNV90498.1"
                     /translation="MHAKHLLHHLVESAAARDPARIAVVAGDRQLRYQELEAALHAFA
                     GGLAGLGLARAERVGIYLDKRPEFVAAAFGTSAAGGVFVPINPVLKAAQVGHILRDCA
                     VRVLVTTAERLAALGDALDHCHDLRHVVLVGADGATTRSVAVHSWQALLAAPARAGHR
                     VIDADMAAILYTSGSTGRPKGVVLSHRNMMAGARSVAHYLGNHPDDTLLAALPLSFDA
                     GLSQLTTAFQAGARVVLLNWLLPRDVLKAIERERVSGLTAVPPLWIQLAQLPWAADTD
                     EHLRYIANTGGRMPGEVLARLRAQLPKTRPFLMYGLTEAFRATYLPPEELDRRPDSIG
                     KAIPDAEVLVLREDGSECAPNEPGELVQRGALVAMGYWNDPERSAERFRPLPAAAPGR
                     EPGLVLPEIAVFSGDTVRRDEDGYLYFIGRRDEMIKTSGYRVSPTEIEEILYATGLVG
                     ECAAFGVAHASLGQSIVAVVTPPAGATLDTAALLAECRSRMPAYMVPARFEPRSGALP
                     RNANGKIDRKALADALAAGEPA"
     gene            15414..16661
                     /locus_tag="PKN25_06060"
     CDS             15414..16661
                     /locus_tag="PKN25_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HNV90499.1"
                     /translation="MNAPARSLPTHAPQTVFAIADGELQIAGQPLSRLAARVGRTPFY
                     ACDRGAIARRVAELRAALPPAIELHYAMKANPMPALVGHLARLVDGIDVASAGELQVA
                     LDAGADPQHVSFAGPGKRDTELRQAVAAGILVNVESFREIAPLAEAAAALGVPARVAV
                     RVNPDFELKSSGMKMGGGPKPFGVDAEQVPGLLAEISRAGLAFEGFHLFAGSQNLKAE
                     AIVEAQRKSFELALRLAAHAPAPVRTLNLGGGFGIPYFPGEQALDLTPIAAGLADIVA
                     EAAQALPEARIVLELGRYLVGEAGVYVCRITDIKVSRGHTYLVTDGGLHHHLSASGNF
                     GQVIRKNYPVAIGNRMDAPPAGEPASVVGPLCTPLDVIADRMPLPAAQIGDLVVVFQS
                     GAYGASASPQAFLGHPQVVEILA"
     gene            complement(16676..17194)
                     /locus_tag="PKN25_06065"
     CDS             complement(16676..17194)
                     /locus_tag="PKN25_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90500.1"
                     /translation="MKPSFLFAGLVLLSGVAAPGVAFAGCGDTQVTGDQLALLLPGRY
                     ACAADERPEWGWKYQELHLASGTLRDFKRGLRGSVDPSKDVGNWRIVGDAVEYSYLDA
                     SGSSGPFSHTVHGNGLGAYSICKGGSEVASLQDDSGNGCVAGGATTMVFQGRSTNTEQ
                     PRTNRGVAAGQR"
     gene            17335..20937
                     /locus_tag="PKN25_06070"
     CDS             17335..20937
                     /locus_tag="PKN25_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNV90501.1"
                     /translation="MHDSYGPPAAQAPRSYLPIALLWALFIAYGSLVPLEFRPRADAW
                     QAFMDTPWLSLGVGSRADWVANVLLYLVLAWFATGAVWTSRLSAWVRTPLLVGVLGTI
                     LALAVGIEYLQLFFPPRTVSRNDLLAEALGTGIGTLLWFAAGPRLAAMWRRFIDGGTH
                     SLRAVLGLYALGYLGLALFPYDFLVSMDELAAKLARPDSLGWLPGLSCGPAFACGIKL
                     LVEAVLMIPFGILLALGVRDHAARRPPGMAAGLAAGALAGVAIEAVQVVLASGTTQGI
                     SVLTRALGTLWGLVLARSGIRRWLEYSPQRLLRAALWLSSVWLALVLATNGLLPLRLQ
                     ASWAALEKLETLRFLPFYYHYYSTETAAVRSLLFVAGSFAPVGVVAALAFPHHRFGAS
                     LLALLVAALVAAAVELLKLFTEGKHPDPTNLLIAVAAAWLAHRLVAHLLPILHHHGTR
                     TTPPTSAAQPRRRVATLLAVGVAPAALLLATVLLGLPLAEPPAVGASAPTYPPPSALP
                     PADIAGFRTAHPRLPHPSPADLAALRAGNPAYLQQTASAARSNPNALFAITLAAFVQP
                     GSVDLAPLHARLVASRFSDRGSGQVEPLALAYDWLHDQWSAQERESLRERLAEGCDFL
                     IEVIRKEQLSPYNAFLYNTPLQGLMACSIALYGDHPRGEAFMRFTHELWKKRVLPVWR
                     QVFGRHGGWHEGGEYVAVGIGQAIHTLPALWRTATGEDLFASEAGIRGFLDFLVYRTR
                     PDRTHMRWGDGAWFDRHPRDAAALALEYRHAAAYTLAPPNAARARDGRRVGPVPTGWP
                     WGPLSDDGLIDPAAQTRMPLARLFDGIGLLVARSDWSEDATWLSFKAGDNFWSHSHLD
                     QGAFTIFKGGPLAIDSGWYGPAYGSNHHMNYTYQSIAHNLVTVTDPADEQPGPGFDAA
                     NPRHYPNDGGQRRIGSGWGVDAAPLDVAQWQERSETYHTGRIAAHLDDDDLVVAVADV
                     GAAYTNRNSGRGSFADRTRRVERMWRVLGYDRINDAVVVFDDVVASRAGFAKRWLLHA
                     VEPPLVRGDRFDLFIPGDTRPGRRGGSLHGHVLLPRDAVLDTVGGPGFEFFVDGRNHD
                     EDGKVQAAIAKLGHGRAEPGAWRIELRPRAAAAEDRFLVVMLPTLAGDQPQARVRLLE
                     AGAEVGAEIAGPRRTTRWWFVPGRLGARVEVLEDGRTRSREIVPGGSPAGNITD"
     gene            21065..21385
                     /locus_tag="PKN25_06075"
     CDS             21065..21385
                     /locus_tag="PKN25_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNV90502.1"
                     /translation="MSTPPTPDAEEIRHALRQVMDPEVGMNIVDLGLIYRIDCAPGEL
                     YIEMTMTTPACPLSDMILDNIDAVLDPLLPPEVDIRVELVWDPPWSPAMMEEGARAHF
                     GWNG"
     gene            21382..22536
                     /locus_tag="PKN25_06080"
     CDS             21382..22536
                     /locus_tag="PKN25_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90503.1"
                     /translation="MSTLPPLARVPLLVLGLLSLLTGVFAGLARLGVEVPALAAVHAG
                     NHAALMICAFFGTVISLERAVALGGLWPYAGPAAAGTGGVLLLAGGPLVAAQLLFLLA
                     AATLFGGGVVVVRRQLALFTVMLAVGAACWLLGSLVWWNTGNVHAAVPLWLAFLVLTI
                     AGERLELTRFLPARPAAAPSFVAVSALILAGAVLAAWEEDLGHGLFAAGVLAMAAWLL
                     TFDIARHNARQQGLTRFIAVCLLSGYVWLAFGALAALGGGLAPAHPLHDITMHAIALG
                     FVFSMVFGHAAIIFPAVLKVKIPYHPAFYVPLALLHASLALRVAGSLAGLPALRSWGG
                     IANALTLAVFIATMVVSVIRGGRPPARRRRNPVPGTPVRSAVKPSGNPAP"
     gene            22533..23009
                     /locus_tag="PKN25_06085"
     CDS             22533..23009
                     /locus_tag="PKN25_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HNV90504.1"
                     /translation="MNRTLRSPLFRKLGRCLSALACGAALGLAAPALASPDEVVMYKD
                     PNCGCCGKWAEHMRSAGFAVKEVASPRMDLVKQEAGVPEALGSCHTAKVGGYVVEGHV
                     PAADVRRMLAEKPAIRGLSAPGMPLGSPGMEGPYPAERYDVVSFDAQGRSAVFSKH"
     gene            complement(23006..24049)
                     /locus_tag="PKN25_06090"
     CDS             complement(23006..24049)
                     /locus_tag="PKN25_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNV90505.1"
                     /translation="MEIEQLRLRLRALGARPSHEARLLRAWLQGHALDAGPRTESNRL
                     PKALQAALPELEAELGALARVRSEHPGEDGTRLLVELADGQAVESVLLPRDGLCVSTQ
                     VGCAVGCVFCMTGKDGLLRQLDSGEIVAQVALARRLRRVHKVVFMGMGEPAHNLDAVI
                     EAIELLGTEGALPHKNLVLSTVGDLRVFERLPQMRVKPALALSLHSTRAALRAALLPR
                     APRIDPAELVELGEAWARASGYPIQYQWTLIEGVNDGEDELEGIVRLLAGKYAVMNMI
                     PFNRVDGLDYRRPAAESAAEIARRLHRAGILTKLRQSAGQDVDGGCGQLRAREAKLAF
                     VRREERRAARPPA"
     gene            24166..>24386
                     /locus_tag="PKN25_06095"
     CDS             24166..>24386
                     /locus_tag="PKN25_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNV90506.1"
                     /translation="MTALRFEGAQDYVATPDLMLAVNAAIRLQRPLLIKGEPGTGKTM
                     LAEQVAAALGLPLLQWHIKSTTKAQQGLY"
BASE COUNT         3570 a         8756 c         8270 g         3790 t
ORIGIN      
        1 gagcgggcgc aggccggagg tggcgtttcc tttcctcgcg gcggcggagg tctagcgccg
       61 cgtgcgcccg gcgacgcgtt gcggcgggcc gaacgcatcg cggaggaggc cgcgtcaggc
      121 cgcgcgcgcg gcctcgccgg ctgccgcatc ctcggcagcg agcgtggcgc agccacagcc
      181 ggcggcgcgc gtctcgcctt cggcgagtgg gcgcagcgtg cacgaatcga cgtcgaagcc
      241 gatcgtcgcc agatgaaagg cgagcgcggc atccatgccg ttcacgtgat tcacgaacca
      301 ggccggcagc tcttccatca gtcggcggcc aaggaagtgg tcgccgcgct cgacgcgctt
      361 gcggatgtcg gccatcaccg cgagcacgcg gtcatgctcg gcgcgatggc agccgggaaa
      421 gtcgaccgct tccatccagc cgttctcgag atcgaagtgg gccttcgcat gctcgatgag
      481 cgcgtcgagc gcacgcagga aatcatcggg cgcggcgttg gccgcggcgt tgtagcactc
      541 gacgaactcg cgatgggtct cgtccatcgg ggcgaggttg ttgtcgagat cggcgctcca
      601 ggcaaggcta ggcatcggtg ttcctcactt gacgatcttg agatggctgc cgcgcggcgg
      661 gcgcgcgggg ggctgcggct ccgcggagcg ctcgcgacgc agttcggcgg tggtgtcgct
      721 catgtcctgg acgacgtcgg cggacggatc ctgcaccgcc acggacgcat cctccaccgc
      781 ctcgagctcg ggctcgaagg ccatgccctg cccgttctcg cgtgcgtaga tcgcgaccac
      841 gttggccacc gggatcacca gcgaatgcgc ggcaccgccg aaacgggcct ggaagctgat
      901 gaagtcgttg gccatttgca gctggccggt ggcgtcgggc gaaaggttca gcacgatctg
      961 gccgtcgcgc gcgtaaccgg gcggcacccg ggtgtcctcg tccaccagcg tggcgagata
     1021 gggcgtgaaa ccctgatcca cgcaccattc ccagatggcg cgcaacaggt atggcttggt
     1081 cgagacggtg ctcatcggct ctccggtggc cgtgccacgc ccctgcgggc gccgcacggc
     1141 ctgactgcgg tcgtgcggct cagcgccgca tgaccttttc ggacggggtc agcgcgtcga
     1201 tgaatccctg acggctgaag atgcgttcgg cgtacttcat cagcggcgca gcagccttgg
     1261 gcagttcgat gccgtagtgc tcgaggcgcc acagcagcgg ggcgatcgcg acgtcgagca
     1321 tggagaactc ctcgccgagc atgtatttct gcttggtaaa caccggcgcg agctgggtga
     1381 gatggtcgcg cacgtgggcg cgcaccttgt ccacgccctt ctgcgcggcc tccagggcct
     1441 ccaggtgggt gaacagctcc tgctcgaaag tgtgcaacag ctggcgggca cgcgcacgca
     1501 tgatcgggtc gggtggcatc agctgcggat gcgggaagcg ctcgtcgatg tactcgttga
     1561 tgatgttggc ctcgtagagc acgaggtcgc gatcgaccag caccggcacg cggttgtacg
     1621 ggttgatgac ggcgatgtct tcgggcttgt tgtagaggtc gacgtcgatg acctcgaagt
     1681 ccatgccttt ttcgaacagc acgatccggc aacgatgact gaagggatcg gtcgtgccgg
     1741 aatacaggtt catcatggtg ttacgactcc ggaattcaaa agcaaaagcg caccacgcgg
     1801 gtccgcgtgg tgcgtcaggg tcaggaccgc actagccggc ccggagggcc gcggcgacag
     1861 gaatcaatga atgtccttcc agtagttctt cttcagcgcg tagctgagca cgaacatgcc
     1921 ggcgaggaag atcagcacga gggcgccgat cgacttgcgc ttctcggcca ccggctcgcc
     1981 catccagacc aggaaggaga cgaggtcggc gaccgccttg tcgtactcct ggaccgacag
     2041 cttgcccggc ttgacgagtt cgagggccgt cgtcgtcgtg ccgtccgcat tctgcgtgat
     2101 cttggcgacc tgctcgccct gcagctgcca gaacacgtgc ggcatggcca cgttggcgaa
     2161 gatggcattg ttccagccgg tcgggcggct cgggtcgcgg tagaactggc gcagataggt
     2221 atacagccag tcggccccgc tgccgtccgc cgaggcgcgc tgccgcgcca ccagcgtcag
     2281 gtcgggcggc gcgacgccga accacccctt ggactccgcc ggccgcatcg cgatcttcat
     2341 caggtcgccg acgcgctcgc cggtgaacat caggttgtcg cggatctccg cctcggagag
     2401 gccgatgttc tgcagctggt tgtagcgcac gaagctggcg ctatggcagt tcatgcagta
     2461 attgacgaac agcttggcgc cgtgctgcag cgcggccggc tcgctgctca ccggcgcctt
     2521 gtccagatgc acgctggcac cggcggccat cgccacggcc ggcgcgaaca gcgccacggc
     2581 cgcgaaccgc ttgatgaagt tgatcactcg cgttttcatt tgaagttcac cctttccggt
     2641 tcgggtttgc acttgtcgag cttgctgtac cacggcatca gcaggaagaa ggcgaagtag
     2701 atcaccgagc agatctgcga caccagcgtg cgacccgggg tcgggggcag aacacccagg
     2761 taacccagga tgaagaaggc gatcacgaag acgaccagca ccatcttgaa gatcggcccc
     2821 ttgtagcgga tcgacttgac ggggctgcgg tccagccagg gcaggaaggc gatgatcacc
     2881 accgacgcgc ccatggccac cacgccccag aacttcgcat cgagaccgaa cagcggatag
     2941 gtcacggcgc gcaggatcga atagaagggg gtgaaatacc acaccggcgc gatgtgcggc
     3001 ggggtcttca gcggatcggc cgggatgaag ttgttgaact cgaggaagta gccaccgccc
     3061 tcgggcatga agaacacgac cgcgctgaag aagaacagga agacgacgac gccgacgatg
     3121 tccttcaccg tgtagtacgg gtggaagggg atgccgtcga gcggcttgcc ggtcgcatcc
     3181 ttcttcttct tgatctcgac accatcgggg ttgttggagc cgacttcgtg cagcgcgacg
     3241 atgtgcgcga gcaccaggcc gatcagcacc agcggcacgg cgatgacgtg gaaggagaag
     3301 aagcggttca gcgtggcgtc ggagacgacg tagtcgccgc ggatcaccag cgacaggtcc
     3361 ggaccgatca ccgggatcgc cgagaacagg ttcacgatca cctgggcgcc ccagaacgac
     3421 atctggcccc acggcagcag atagcccatg aacgcctcgg ccatcaacac caggaagatc
     3481 agcgtgccga agacccagat caactcgcgc ggcttgcgat aagagccata gagcagaccg
     3541 cggaacatgt gcagatacac gacgatgaag aaggccgacg caccggtcga gtgcaggtag
     3601 cggatcagcc agccgcccgg cacctcgcgc atgatgtact cgacgctggc gaacgccacc
     3661 ggaacgcccg acgcgttgag cgaggcgtcc ggcttgtagt gcatcaccag gaagatgccg
     3721 gtgaggatct ggatcaccag caccagcagg gccagcgagc cgaagaagta ccagaagttg
     3781 aagttcttcg gcgcgtagta ctcggacagg tgcgccttcc aggtcgacgt gagcgggaag
     3841 cgctcgtcga tccagttgag aacggcttgg gatttggtcg tcatcgctta tgccttcccg
     3901 tcatcgccaa caaggatttt ggtgtccgcg agatactggt gcggcggcac ctcgagattg
     3961 tccggcgcgg gcatgctgct atacacgcgg ccggccagat cgaagatcga gccgtgacag
     4021 gggcacagga agccacccgg ccagtcggcg cccatgccgc tgtccgcgcc caccttgaac
     4081 ttctcggagg gcgagcagcc gaggtgggtg cagatgccga ccgtgaccag gtactccggc
     4141 ttgatcgagc ggtgcttgtt cttggcgtac tcgggctgct gcggcttcac ggagttgggg
     4201 tcgctgacct tttcgtccgt cttgtcgagc gaggcgagca tctcgggcgt gcggcgcagg
     4261 atccacaccg gcttgccacg ccactccacc gtcatcatct cgcccggctt cagcttgctg
     4321 acgtccgcct ccaccggcgc accggccgcc ttcgcgcgtt cggacggcgt caggctggcg
     4381 acgaacggca ccgcggtcgc cactgcggcg acgccacctg cggcggacgt cgccagcagc
     4441 agggtgcggc ggctgctgtc cattttctga tcaacgctca tgagcctctt cccaaaaagg
     4501 aaaacgaaag acctggatac aaataaccat ccgattatat cgaagacgct gcagcagaga
     4561 aagcgcgacg ggccgatcgc cagccggaac aaggccttcg gcacgaaaac cgacaccgat
     4621 caggaaccgc ccggggcatg cgttgctgca ttgcagcaac ggaaattcag accgcgcggc
     4681 gctcgatcag agcctggccg atggtgccga tgtcggtatg ctcgagctcg ccgccgaccg
     4741 ggacgccgcg cgacagccgg ctgaccttga ccccgcgcgc gccgagcagg ctcgcgacgg
     4801 tgtgcgcggt ggcctcgccc tcgttggtga agttggtggc gaggatgacc tcgcgcaccc
     4861 cctcctcggt ggcgcgcgcg atcagcttgt ccaggcgcag ttcgcgcggc ccgacgccat
     4921 cgagcggcga aagccggccc atcagcacgt agtagaggcc gttgtaggac tgcgtctgct
     4981 cgatcatggc gagatcggcc ggcatctcca ccacgcacag cagcgcggcg tcgcggcgcg
     5041 gattggcgca gcgcggacac acctcgtcct cggtgaagct gttgcagcgc gcgcaatggc
     5101 gcagcacctc gagcgcatgc agcagggcat tgcccagccg cgccgcgccg cgctgatcac
     5161 gctgcagcag atgataggcc atgcgctgcg ccgacttggg gccgaccccg ggcaggcagc
     5221 gcagtgcctc gatcagttcg tcgaggctgg aaggcgacat cggcccgccg ctcagaacgg
     5281 cagcttcatg cccggcggca ggttcagccc ggcggtgacg ctggacatct tctcctgggt
     5341 ggccgcctcg accttgcgca cggcgtcgtt gagcgctgcg gcgacgaggt cctcgagcat
     5401 ctcgcggtcg tccatgaccg actcgtcgat ggacacccgg cgcacgtcgt acttgcaggt
     5461 catctgcacc ttgaccatgc cggcgccgga ctgcccctcg acctcgatcg agccgagctg
     5521 gtcctgcatc ttcttcatgt tttcctgcat ctgctgggcc tgcttcatca ggcccgcgat
     5581 accgcctttc atcatgatgg tgttctccgt cggatcggtt gtcgggtggg gtcgatcggg
     5641 agggcgcgcc tcccggcatc gaaggggttc aaaggggttt caccgtgttc tcgacgagcg
     5701 tggcgtcgaa acgttcgatc agctcgcgca cgaaggggtc ggcctcgagc gctgcgaccg
     5761 cctcggcatg gcggacctgg cgctcgatcg cgtcgcgctg cgcgggggtc tgtccggaga
     5821 tgtcgccgat cgcgatgcgc accgcgagcg tgcgtccgag cgcggcgcac agcacgtcct
     5881 gcaggcgctc gaccgcgccg cgattcatgt cgagcaggtg gcggtgcgcg gtcgacaggc
     5941 gcagctgcag cacgccatcg ccacactcga cccattcgca gtgctgggca agctcgcgca
     6001 ccagtccgcc gagcccgagc gcgcggatca ggccgcgcca gtcgccgagc gcaagcagcg
     6061 ccgcggcatc gccactggcg gcgggcgcat cctgggtcgg cgcagccgga gacgtcgccg
     6121 gctcgggcgc gcgcaccgcg ggaaggggcg cggaccgcga cgggacttcc tccgcccgcc
     6181 gcgcgccacc gccacgcggc tccgggcgtg cccgtgagat cgcccccgtg tgcgcacccg
     6241 cggaggcggc gtaggcctcg tcgtggccgt tgcgcgcctc gcgcggcggc ggcagatcgg
     6301 aatcggcata ggcctcgggc gggagatcct cccagggcgg aacgtcctgc acgcttgccg
     6361 gggcagcacg cgccggctgc acggcgtgct cctcgcgcgc ggatctgcgc ggcggcgcgc
     6421 tcgcaggcgc aggtgcgacg gcctgctgcg ccgccggaac ggagccgggc tgcatggatg
     6481 cgggcgccgc agcctcgagg acggtctgct gcgcgctggg gatcgccgca tcggcgccgg
     6541 ccgccggtgc ggggacggcc gccggtgcgg gaccggcgat cccggccgac gcgacggtcc
     6601 gcgcgggcac ctcggccgga acgcccgact ccgcggcacg cccggactcc gccgggccgc
     6661 cggccgccag tgcaggcgcg cgcgcgcgcc ccgcatccgg cccggatggc acgcccggcc
     6721 cgccaagcgc agcaggctgc tccgggcgga acgcatgcag gcgcagcagg gtcatcgtga
     6781 agccggtgta gtcgtccggc gccagcggca gctcgtcgcg gccgtggacg gcgacctggt
     6841 aggcgagctg gaggaactcg gcgtcgaagc gctcgacgta gccgaggatg cgcgcgcgct
     6901 cgagctcgtc ggcgatcgcc gcaggtgcgt actgggcgag cgcgacgcga tgcaccagcg
     6961 tggccagcgc ctgcagtgcg gcgtcgaagg acaggctcct cgcctccatg ccgtcggcca
     7021 ccgcgagcag cgcgggcacg tcgcccgcgg cgagcgcgtc gagcaccgcg tagaggtggt
     7081 cgtcccccac cgtgccgagc atgtggctga cctgctcctc ttccacccgg cccgcgccat
     7141 gcgcgatcgc ctggtcgagc agcgacagcg cgtcgcgcat cgagcccgac gccgccttgg
     7201 cgaggtgacg cagcgcgccc ggctcgaacg ccacctcctc ggcctgcagg atgcgcgtca
     7261 ggtgatcgac gatatggccc tgcggcatct gcttgaggtt gaactgcagg cagcgcgaca
     7321 gcaccgtgac cgggatcttc tgggggtcgg tggtggcgag gatgaatttc acatgctccg
     7381 gcggctcctc gagcgtcttc aacatggcgt tgaaggcgtg cccggtgagc atgtgcactt
     7441 cgtcgatcat gtagaccttg aagcgcccct gcaccggcgc atacaccgcc ttgtcgagca
     7501 gcgcggccat ctcctcgacg ccgcggttcg aagccgcatc catctcgacg tagtcgggga
     7561 agcggtcggc atcgatcgcg cggcaggcgt cgcaaatgcc gcagggctcg gcggtgatgc
     7621 cggtctcgca gttgagtgcc ttggcgagga tgcggctgat ggtggtcttg cccacgccgc
     7681 gcgtgccggt gaacagccag gcgtggtgca gccgcccggt ggcgagcgcg tgcgtcagcg
     7741 cacgcacgac gtgctcctga ccgaccagcg tgccgaagga tttcggccgc cacttgcgtg
     7801 cgaggacctg atagctcatg gcgggcgatt ctagcagagc ggcgggcggc gacccgcgcg
     7861 ctcgacggga cgcaggttca gccccccggc gtccggctca gcatgccgcg ctcgcccagc
     7921 caggcaagca ccgcatcggc cgcggcctcc aggtcgaggc gggtggtatc gaggcgcagt
     7981 tcggggtcct ccggcgcctc gtaaggcgaa tcgatgccgg tgaagttctt cagctcgccg
     8041 cgccgggcct tcttgtacag gcccttggga tcgcgcgcct cggccacctc cagcggagtg
     8101 tcgacgaaga cctcgacgaa ctcgccctcc tccaccaggc cgcgcgccat gcggcgctcg
     8161 gagcggaaag gcgagatgaa ggcggtgagc acgatcaggc ctgcatcgac catcagcttc
     8221 gcgacctcgg cgacgcgacg gatgttctcc acgcgatcgg cgtcggtgaa gccgaggtcc
     8281 ttgttgaggc catggcgcac attgtcgccg tcgagcaggt aggtgtggtg gccgagcgcg
     8341 tgcagtttct tctcgaccag gttggcgatc gaggacttcc ctgcgccgga caggccggtg
     8401 aaccacagca cgcagccgcg ctggttcttc agcgcggcac gcgcgcgctt gtccacatcg
     8461 acgtgctgca ggtggatgtt gtgcgcgcga cgcagcgcga agtgcaacag gccggcgccg
     8521 acggtgttgt tggagaggcg gtcgatcagg atgaagccgc cggtgtcgcg gttgatccgg
     8581 tagggatcga aggcgatcgg ccgatccagg ctcaggttgc acacgccgat cgcgttgagc
     8641 tcgagccgct tggcggcgac gtgctcgagg gtgttcacat tcacctggta cttgatctcg
     8701 gtgatcgtcg cgctgaccgt gcgcgcgccg atcttgagca ggtaagggcg gccggcgagc
     8761 atgggctcgt cgtgcatcca caccaccgtg cactcgaact ggtcggcgac ctcggccggc
     8821 gcctcgacgg tcgagatgac gtcgccgcgc gagatgtcga tctcgtcggc gagggtcagc
     8881 gtgaccgact gcccggccac ggcccggtcg agatcgccgt cgcgggtcac gatgcgcgcc
     8941 accgtgctct cgcgccccga gggctgtacg cgcacacgat cgcccgggcg gatggtgccg
     9001 ccggcgatgg agccggcaaa gccgcggaaa tccaggttgg ggcgattgac ccactgcacc
     9061 ggcaggcgga agggcgcgcg ctgcatcagg ccgtcatcga cctccaccgt ctcgaggtag
     9121 cccatcagcg tggtgccgtg gtaccagggc atcgcagcgc tcggagaggt gatgttgtcg
     9181 cccctgaagg ccgacatcgg gatgaaggtc acctgctcga tgccgagctg gcgggagaag
     9241 gcggtgtagt cctcgacgat gcggcggaac acgccctcgg cgtaatcgac caggtccatc
     9301 ttgttgatcg ccaccacgat gtggcggatg ccgatgaggt tcaccaggta gctgtgacgc
     9361 cgcgtctggg tgaggatgcc cttgcgcgca tcgaccatca ggatggccgc gtcggccgtc
     9421 gaggcgccgg tgaccatgtt gcgggtgtat tgctcgtggc cgggggtgtc ggcgacgatg
     9481 aacttgcgct tgtcggtgga gaagaagcga tacgccacgt cgatcgtgat gccctgctcg
     9541 cgctcggcgg ccaggccgtc gaccagcagc gcgaagtcga tgtcgccgcc ctgcgtgccc
     9601 cacttcttgc tgtcggcctc gatcgcggcg agctggtcct cgaacagcat cttggcgtcg
     9661 aacagcaggc ggccgatcag cgtgctcttg ccgtcgtcca cgctgccgca ggtgatgaag
     9721 cgcagcaggc tcttgttctc gtgcgccctg aggtactgct cgatgtcggt cgcgatcagg
     9781 tcggaaacgt gtgccattta gaaatagccc tcctgctttt tcttttccat cgaggcggcc
     9841 gagtcgtggt cgatcacccg cccctggcgc tccgaggtcc tggccagcag catctcctgg
     9901 atgatcgccg gcagggtgtc ggcctcggac tcgatcgcgc cggtgagcgg atagcagccc
     9961 agagtgcgga agcgcacctt cttcatcatc ggcacctcgc ccgggcgcag cggcatgcgc
    10021 tcgtcgtcga ccatgatcag cgtgccgtcg cgctcgacca ccgggcgcac ggccgagtag
    10081 tacagcggca cgatcgggat gttctcgagg tagatgtact gccagatgtc gagctcggtc
    10141 cagttcgaca gggggaacac gcggatcgac tcgcccttgt gcttcctcgc gttgtagagc
    10201 ttccacagct cggggcgctg gttcttcggg tcccagcggt gctgcgcggt gcggaaggag
    10261 aacacgcgct ccttggcgcg cgatttctcc tcgtcgcggc gcgcgccgcc gaaggccacg
    10321 tcgaagccgt actggtcgag cgcctgcttg aggccctcgg tcttcatgat gtcggtgtgg
    10381 atcgccgagc cgtgggtaaa ggggttgatg tccttctcca cgccctcggg gttgatgtgc
    10441 accagcaggt ccatgccggt ctcgcccgcc atgcggtcgc gaaagctgta catgtcgcgg
    10501 aacttccagc gcgtatcgac gtgcagcagc ggaaagggcg gcaccgccgg atggaaggcc
    10561 ttcagggcca ggtgcagcat caccgcgctg tccttgccga tggagtagag catcaccggg
    10621 ttgtcggcct cggcgacgac ctcgcgcagg atgtggatgc tctcggcttc gaggcgctgg
    10681 aggtgggtca ggctggtcgt aggcacgctg gtcatgggcg acgggatgcg aagggacaag
    10741 gcatgaatga tgaggccgcg cgccgtcgcg cgcagcgtct tcgaagatcg gcgggcggaa
    10801 ctatttctcg cggtgcgaca ttctgcaccc gaatccacct ctagacgggg ctctcgccac
    10861 gcccgcccgg gtacacttgc ggcgtcgacc actcccccgg agcccgctga tggacaccgc
    10921 aaccgcctca cggctcctcg aagccctgct cccgatcgtg cgcgcggccg gcgcgctggt
    10981 gatggacatc taccgcagcg acttcgcggt gcgcggcaag gacgacgcct cgccggtaac
    11041 cgaggccgac gagcgcgccg aggcgctcat cgtccccgcc ctcgaggcct tgctgcccgg
    11101 cgtgccggtg gtggccgaag aggccgtcgc ggcgggccgc ctgcccgcac tcgggcgccg
    11161 cttctggctg gtcgatcccc tcgacggcac caaggagttc atcggccgca acggcgagtt
    11221 caccgtcaac atcgccctcg tcgaggatgg cgagccggtc ctcggcaccg tcttcgcacc
    11281 cgcgctcgag cgcctcttcc tcggcgcggg cggggtcggc gccttcgtcg agcaggacgg
    11341 ccgtcgccgc ccgatccgct gccgcaccgt cccgcccgcc ggcctcaccg tggtggccag
    11401 ccgctcgcac ggcgacgcgg ccgcgctcga cgccttcctc gacgggcgca aggtggccgc
    11461 gctcaccaac gccggctcct cgctcaagct gtgcctggtc gcggcgggcg aggccgatct
    11521 ctatccgcgc ctcggccgca ccatggaatg ggacatcgcc gccggccatg cggtgctggc
    11581 cgccgccggc ggccgcgtgc aaaccctggc gggggcgccg ctgcgctacg gcaagccggg
    11641 gctggacaac ccccacttcg tcgccagcgg actggactga gcccgggcgt gccgatgacg
    11701 accgcccacc tcgtcctcgc ctcgatcgcg ctgctgctgg cgctgctggc ccacggccgc
    11761 gtcggcccgg cggtgctgtt cgcggcctgg gcgggcggct accacctgct cggcctcgcc
    11821 agccagcagg aactgctggc gagcttctcc aaccccgcgc tcgccaccct gatcctgctg
    11881 ctgctggtct cgctcgcgct cgaacgctcg ccgctgctcg accatctgtc ggaactcctg
    11941 ctcaagggcc gcgtcgggct ggcgaccctg cgcctgtccg tcttcgcctg cctgatctcg
    12001 gcgttcatga acaacaccgc ggtggtgggc gcgctgctgg gcacggtcac ctcgcaacgc
    12061 cgacaccccg cctcgaagct gctgatcccg ctctcctacg cctccatcct cggcggcatc
    12121 accacactgg tcggcacctc gaccaacctg gtggtgaact ccttcgtcat cgacgcgggc
    12181 ctgccgccgc tgcagatgtt ccagttcgcc tgggtaggga ttccggtcgc gctcgcctgc
    12241 atcggggtga tggcactgag ctcgcgactg ctgcccgcga acgcctcgag cgaggccgag
    12301 gcacgctcga cctacttcct cgaggcgcgc gtggctgcgg gctcgccgat gatcggacgc
    12361 agcatcgaaa ccaaccgcct gcgcaacctc gagggcctgt tcctgctcga gatcgagcgc
    12421 gagggccggc tgatctcgcc ggtcggcccg gccgagatcc tgcacaccgg cgacgtgctg
    12481 gtgttcaccg gcgaggtggc gcgagtgcag accctgcagc gcttccccgg gctggaggtg
    12541 ttcggcgcgg gcgccgacgc gctgctcaag tccaacctgg tcgaggtggt gctcgcgagc
    12601 agctcggacc tggccaaccg caccctgcgc gaggtcgact tccgcaccat gttcgatgcc
    12661 ggggtggtcg gcatccgccg cggcgaccgg cgcctgaccg gccagctcgg acgcatcccg
    12721 ctgcgcgtcg gcgacagcct gctgctggcc gcgggcccgg acttcctgca gcaccgcaac
    12781 ctcgaccgca acttccacat cctcaacggc tcgggtctgc gccccaagct cggccgcatg
    12841 caaagcgtcg cggcgctgtc gggcttcgca ctcgtgatcc tggtcgccgc cctgggctgg
    12901 ctgcccctgt tcggcggcct gctcgtgctg ctcgccgccc tgctcggcag cggcctgctg
    12961 accctgggcg agctgcgccg gcgctttccc ttcgaactgc tggtggtgat cggctccgcg
    13021 ctggcgatcg ccggcgtggt cgagcgttcg ggcgcggcgg agctggtcgc gggctggatg
    13081 cgggcgatct tcgacgggca cggcgtccat gccgcgctcg cgggtgtcta cctgatcacg
    13141 ctgacgctca ccgaggtgat caccaacacc gccgccgccg cgctcgcctt cccgatcgcg
    13201 ctcggcaccg cgcgcgcgtt cggggtcgat ccgatgccct tcgtgatggt ggtggcctac
    13261 ggcgcgagcg ccggcttcct gatccccttc ggctaccaga cccacctgat ggtgtattcg
    13321 gccggccgct accgcatgcg ggatttcctg cgcgccggac tgccggtgag cctcacctac
    13381 tcggcgctgg tgctgctgct ggtgccgctg gtgtttccgc tccaccgctg agcacggcgc
    13441 acggaatcag ccgggctggc gagcactcag gcgggtgacg aactcgacca cggtaccgac
    13501 cgtggcgaag gagttgccat cgacctcgtc gtccggcacc tcgatgccga aacgcgcctc
    13561 gatctcctgg atgacggcga tcaccgccat cgaatcgagc tcgggcagat ctcccagcaa
    13621 gggcgtgtct gcggcgaatg cgagcgcgcg accgtccagg ctcagcacct cgtccagcag
    13681 cgacagcact tccttcagaa tctgttcgga catcacttct ccatgacgga agcgggtcac
    13741 gacgcgcatc cacgccgccc gccccgcaaa acgcgcgcat tatacttgcc agcccgttcc
    13801 tgtctgcacc gataagcgaa gccgatgcac gccaaacacc tgctccacca cctcgtcgag
    13861 tccgcggccg cacgcgatcc cgcacgcatc gccgtggttg ccggcgaccg gcagctgcgc
    13921 taccaggagc tcgaggccgc gctccacgct ttcgccggcg gcctggccgg gctgggcctc
    13981 gcccgcgccg agcgcgtcgg gatctatctc gacaagcgcc ccgagttcgt cgccgccgcc
    14041 ttcggcacca gcgccgcggg cggcgtcttc gtgccgatca accccgtgct caaggccgcg
    14101 caggtcggcc acatcctgcg cgactgcgcg gtccgcgtcc tcgtcaccac cgcagaacgc
    14161 ctcgccgcgc tcggcgacgc gctcgaccac tgccacgatc tgcgccacgt ggtgctggtg
    14221 ggcgcagacg gcgcgaccac ccgttccgtc gccgtgcatt cctggcaggc cctgctcgcc
    14281 gccccggcac gcgccgggca tcgcgtcatc gacgccgaca tggcggcgat cctgtacacc
    14341 tcgggcagca ccgggcgacc gaagggcgtg gtgttgtccc accgcaacat gatggcgggg
    14401 gcgcgcagcg tggcgcacta cctcggcaac caccccgacg acaccctgct cgccgccctg
    14461 ccgctgtcct tcgacgccgg gctgtcgcag ctcaccaccg ccttccaggc cggcgcccgc
    14521 gtcgtgctgc tcaactggct gctgccgcgc gacgtgctca aggcgatcga gcgcgaacgc
    14581 gtgagcgggc tgaccgcggt gccgccgctg tggatccagc tcgcgcaatt gccctgggcc
    14641 gcggataccg acgagcacct gcgctacatc gccaacaccg gcgggcgcat gccgggcgag
    14701 gtcctcgccc gcctgcgcgc gcagctcccg aagacccgcc ccttcctgat gtacggcctc
    14761 accgaggcct tccgcgcgac ctacctgccg cccgaggagc tcgaccgccg ccccgactcg
    14821 atcggcaagg ccatccccga cgccgaggtg ctggtgctgc gcgaggacgg cagcgaatgc
    14881 gcgcccaacg agccgggcga gctcgtgcag cgcggcgcct tggtggcgat gggctactgg
    14941 aacgaccccg agcgcagcgc cgagcgcttc cgcccgctgc ccgccgccgc ccccggccgc
    15001 gaacccggcc tggtgctgcc cgagatcgcg gtgttctccg gcgacacggt gcgccgcgac
    15061 gaggacggct acctctactt catcggccgt cgcgacgaga tgatcaagac ctcgggctac
    15121 cgcgtcagcc ccaccgagat cgaggagatc ctctacgcca ccggcctggt cggcgaatgc
    15181 gcggccttcg gcgtcgcaca cgcgagcctc ggccagagca tcgtcgcggt cgtcaccccg
    15241 cccgccggcg ccacgctcga cactgccgca ctgctggcgg agtgccgcag ccgcatgccg
    15301 gcctacatgg tgccggcgcg cttcgagccg cgcagcggcg cgctgccgcg caacgccaac
    15361 ggcaagatcg accgcaaggc gctcgccgac gccctcgccg ccggagagcc cgcatgaacg
    15421 cccccgcccg cagcctgccc acgcacgcgc cgcagaccgt gttcgcgatc gccgacggcg
    15481 aactccagat cgctggccag ccgctctccc ggctcgccgc gcgtgtcggg cggacgccct
    15541 tctacgcctg cgatcgcggc gcgatcgcac gccgggtggc cgagctgcgc gcggcactgc
    15601 cgcccgccat cgagctgcac tacgcgatga aggccaaccc gatgccggcg ctggtgggcc
    15661 acctggcgcg cctggtcgac ggcatcgacg tcgcctccgc cggcgagctg caggtcgcgc
    15721 tcgacgccgg cgccgacccc cagcacgtca gcttcgccgg tcccggcaag cgcgacaccg
    15781 agctgcgcca ggcggtggcc gccggcatcc tggtcaatgt ggaatccttc cgcgagatcg
    15841 cgccgctggc cgaggccgcg gccgcgctcg gcgtgccggc gcgggtcgca gtacgggtga
    15901 atccggactt cgagctcaag tcctcgggca tgaagatggg cggcggcccc aagcccttcg
    15961 gcgtggacgc cgagcaggta cccggcttgc tcgccgagat cagccgcgcc gggctcgcct
    16021 tcgagggctt ccacctcttc gccggctcgc agaacctcaa ggccgaggcc atcgtcgagg
    16081 cccagcgcaa gagcttcgaa ctcgccctgc gcttggccgc gcacgccccc gccccggtgc
    16141 gcacgctgaa cctcggcggc ggcttcggca tcccgtactt ccccggcgaa caggcgctcg
    16201 acctgacgcc gatcgccgcc ggcctcgccg acatcgtcgc cgaggccgca caagccctgc
    16261 ccgaggccag gatcgtgctg gaactcggcc gctacctggt cggcgaggcc ggcgtgtatg
    16321 tgtgccggat caccgacatc aaggtctcgc gcggccacac ctatctcgtc accgacggcg
    16381 gcctgcacca ccacttgtcg gcgagcggca acttcggcca ggtgatccgc aagaactacc
    16441 cggttgcgat cggcaatcgc atggacgctc cacctgccgg cgagccggca tcggtggtcg
    16501 gcccgctctg cactccgctc gacgtgatcg ccgatcgaat gccgctaccg gccgcgcaga
    16561 tcggcgacct cgtcgtggtg ttccagtccg gcgcctatgg cgcgagcgcg agcccgcagg
    16621 ccttcctcgg ccacccgcaa gtggtcgaga tcctcgcctg agggccacaa ggcgttcagc
    16681 gctggccggc cgccacgccc ctgttcgtcc gcggctgctc cgtgttcgtg ctccgaccct
    16741 ggaagaccat ggtcgtggcg ccccccgcga cacaaccgtt tcccgagtcg tcctgcaggc
    16801 tcgcgacctc gctgccaccc ttgcagatgc tgtaggcacc gaggccgttg ccgtgaacgg
    16861 tgtgggaaaa aggcccggag gagccgctcg catcgaggta ggaatactcc acggcatccc
    16921 cgacgatcct ccagttgccg acgtccttgc tcgggtccac gctgcccctc aagccgcgct
    16981 tgaaatcacg caaggtgccg gacgccagat gcagctcctg atatttccag ccccactccg
    17041 gacgctcatc cgccgcacat gcgtagcgac cgggcaacag gagggccagc tggtccccgg
    17101 tcacctgcgt atcaccacat ccggcgaacg cgacacccgg cgccgcgaca cccgacagca
    17161 gaaccagacc ggcaaaaagg aaagaaggct tcatgttcgc acccggcgca atcgaaacga
    17221 tggaggaagc ggcgggcaga cagaaccgga ccaccgcggg ctttgattaa acatagcagc
    17281 tcggcgcaca ccgtacgaca atcggggctc gctcccgcag gaaatatttc tccgatgcac
    17341 gattcctacg gcccccccgc ggcgcaagca cctcgcagct acctcccgat cgccctgctg
    17401 tgggcgcttt tcatcgccta cggcagcctg gtgcccctcg agttccgccc tcgcgccgac
    17461 gcctggcagg cattcatgga cacgccctgg ctgtcgctcg gggtcggttc gcgcgccgac
    17521 tgggtggcga acgtgctgct ttacctggtg ctggcctggt tcgctaccgg ggcggtgtgg
    17581 accagccgcc tctccgcctg ggtgcgcacg ccgctgctcg tcggcgtgct cggcaccatc
    17641 ctcgccctcg cggtgggaat cgaatacctg cagctgttct tcccgccgcg aacggtgtcg
    17701 cgcaacgacc tgctcgcaga ggcgctcggc accggcatcg gcacgctgct ctggttcgcg
    17761 gccggccctc gcctcgcggc gatgtggcgg cgtttcatcg acggcggcac gcacagcctg
    17821 cgcgccgtgc tgggtctgta cgcactgggc tacctcgggc tcgcgctgtt tccctacgac
    17881 ttcctggtga gcatggacga gctcgccgcc aagctcgccc gccccgacag cctcgggtgg
    17941 ttgcccggcc tgtcctgcgg acccgcattc gcctgcggca tcaagctgct ggtcgaggcc
    18001 gtcctgatga ttcccttcgg catcctgctc gcgctcggtg tgcgcgacca cgccgcgcgg
    18061 cgccccccgg ggatggcggc cggtctcgcc gcgggcgccc tcgccggcgt ggcgatcgag
    18121 gcggtgcagg tggtgctcgc gtccggaacg acacagggca tctcggtgct cacccgcgcg
    18181 ctcggcaccc tgtggggact cgtactcgcg cgtagcggaa tacggcgctg gctggagtac
    18241 tcgccacaac gcctgctgcg cgcggcgctg tggctctcgt ccgtctggct cgccctggtg
    18301 ctggcgacga acggcttgct gccgctgcgg ctccaggcct cctgggctgc gctggaaaaa
    18361 ctcgagacgc tgcgcttcct gcccttctac taccactact acagcaccga gacggccgcc
    18421 gtgcgcagcc tgctcttcgt ggcgggaagc ttcgcaccgg tgggggtggt cgccgccctc
    18481 gcctttccgc accatcgctt cggcgccagc ctgctcgcgc tgctggtcgc cgccctcgtc
    18541 gcggcggcgg tcgagctgct caagctgttc accgagggca agcatcccga ccccaccaac
    18601 ctgttgatcg cggtcgcggc cgcctggctc gcgcaccgcc tcgtcgcgca cctgctgccg
    18661 atcctgcacc accacggcac gcgcacgaca cccccgacgt ccgctgcgca gccccggcgc
    18721 agagtcgcga cgctgctcgc cgtgggtgtg gcgccggcgg cgctgctgct cgccacggtg
    18781 ctgctcggcc tgccgctggc cgagccgccc gccgtggggg cgtccgcgcc cacgtacccg
    18841 cctccttcgg ccctgccacc ggccgacatc gccggctttc gcaccgccca cccccgcctg
    18901 ccccacccct cccccgccga cctcgcggcg ctgcgcgccg gcaaccccgc ctacctgcag
    18961 cagacggcga gcgcggcgcg cagcaacccg aacgccctct tcgcgatcac gctggcggcc
    19021 ttcgtccagc ccggcagcgt cgatctcgcc ccgctgcacg cgcgcctggt ggcgagtcgc
    19081 ttcagcgacc gcggcagcgg ccaggtcgaa ccgctcgcgc tcgcctacga ctggctgcac
    19141 gaccaatgga gcgcgcagga acgcgagagc ctgcgcgagc gcctcgccga gggctgcgac
    19201 ttcctgatcg aggtgatccg caaggaacag ctctcgccct acaacgcctt cctctacaac
    19261 accccgctgc aaggcctgat ggcgtgcagc atcgcgctgt acggcgacca tccgcgcggc
    19321 gaggccttca tgcgcttcac ccacgagctg tggaagaagc gtgtgctgcc ggtctggcgc
    19381 caggtcttcg ggcgccacgg cggctggcac gaaggcggcg aatacgtggc ggtgggcatc
    19441 ggccaggcca tccataccct gcccgcgctg tggcgcaccg ccaccggcga ggacctgttc
    19501 gccagcgaag cggggatccg cggcttcctc gacttcctcg tctatcgcac ccgccccgat
    19561 cgcacccata tgcgctgggg cgacggcgcc tggttcgacc gccatccgcg cgacgccgcg
    19621 gcgctcgccc tcgagtaccg ccacgccgcc gcctacacgc tggcaccgcc caacgcggcg
    19681 cgcgcgcgcg acggccgtcg ggtcggcccg gtgcccaccg ggtggccatg gggaccgctg
    19741 tcggacgacg gcctgatcga ccccgccgcg cagacccgca tgccgctcgc gcgcctgttc
    19801 gacggcatcg gcctgctcgt ggcgcgcagc gactggtcgg aggacgccac ctggctcagt
    19861 ttcaaggccg gcgacaactt ctggtcgcac agccacctcg accagggcgc gttcacgatc
    19921 ttcaaggggg ggccgctggc gatcgacagc ggctggtacg gtcccgccta cgggtcgaat
    19981 caccacatga actacaccta ccagagcatc gcccacaacc tggtcacggt gaccgacccc
    20041 gccgacgagc agcccggccc cggcttcgac gccgccaacc cgcgccacta ccccaacgac
    20101 ggcggccagc gccgcatcgg ctcgggctgg ggcgtggatg cggcgccgct cgacgtcgca
    20161 caatggcagg agcgcagcga gacctaccac accggccgca tcgcggccca cctcgacgac
    20221 gacgacctgg tcgtcgccgt ggccgacgtc ggcgccgcct acaccaaccg caactccgga
    20281 cgcggcagct tcgccgaccg cacccgccgc gtcgagcgca tgtggcgggt gttgggctat
    20341 gaccggatca acgatgcggt ggtggtgttc gacgacgtcg tcgccagccg tgccggcttc
    20401 gccaagcgtt ggctgctgca tgcggtggag ccgccgctcg tgcgcgggga ccgcttcgac
    20461 ctgttcatcc cgggcgacac ccgcccgggg cggcggggcg gcagcctgca cggccacgtc
    20521 ctcctgccgc gcgacgcggt gctcgacacc gtaggcggcc ccggcttcga gttcttcgtg
    20581 gacgggcgca accacgacga ggacgggaag gtgcaggcgg cgatcgcgaa gctcggccac
    20641 ggccgcgccg agccgggcgc ctggcgcatc gagctgcggc cgcgcgccgc ggccgccgaa
    20701 gaccgcttcc tcgtcgtgat gctgcccacg ctcgccggcg accagcccca ggcccgcgtg
    20761 cgcctgctcg aagccggcgc ggaggtgggt gcggagatcg ccgggccgcg gcgcaccaca
    20821 cgctggtggt tcgtgcccgg acgcctgggc gcacgcgtgg aggtgctcga ggacggtcgc
    20881 acgcgcagcc gcgagatcgt gcccggcgga tcccccgccg gaaacatcac ggattgaagg
    20941 attcgccgac ctggccgaca atgccccacg caccgcggcg ggctgcacag cgcccgccga
    21001 ccgccgcaaa cccgcttgca gccgtcccgg cgacggtcgc ggcccgcacc ctggacgccc
    21061 ccacatgagc acaccgccca ctcccgacgc cgaagagatc cgccacgcac tgcgccaggt
    21121 catggatccg gaggtcggca tgaacatcgt cgacctcggg ctgatctacc gcatcgactg
    21181 cgcacccggc gagctctaca tcgagatgac gatgaccacg ccggcctgcc cgctctccga
    21241 catgatcctc gacaacatcg acgcggtgct cgaccccctg ttgccacccg aggtggacat
    21301 ccgcgtcgag ctggtgtggg atccgccctg gtccccggcg atgatggaag agggcgcacg
    21361 cgcgcacttc ggctggaacg gatgagcacc ctgcccccgc tcgcccgtgt ccccctgctg
    21421 gtgctcgggc tgctgtcgct gctcaccggc gttttcgccg gcctcgcccg cctgggcgtg
    21481 gaggtgccgg cgcttgccgc cgtgcatgcg ggcaatcacg cggcgctgat gatctgcgcc
    21541 ttcttcggca ccgtgatcag cctcgagcgc gcggtcgcgc tcggcggcct gtggccctac
    21601 gccgggccgg ccgccgccgg gacgggtggc gtgctgctgc tcgcgggtgg tccgctggtc
    21661 gccgcgcagc tccttttcct gcttgccgcg gcgaccctgt tcggtggcgg cgtggtggtg
    21721 gtgcgccggc agctggcgct attcaccgtg atgctcgcgg tgggcgcggc atgctggctg
    21781 ctgggcagcc tggtgtggtg gaacaccggc aacgtgcacg cggcggtgcc gctgtggctc
    21841 gccttcctgg tgctgacgat cgcgggcgaa cgcctcgagc tcacccgctt cctgcccgca
    21901 cgcccggccg cggcgccgag ctttgtcgcg gtcagcgcgc tgatcctcgc cggcgccgtg
    21961 ctcgcggcat gggaagaaga cctcggccac ggcctcttcg ccgccggcgt gctcgcgatg
    22021 gcggcctggc tgctgacgtt cgacatcgcc cgccacaacg cccgccagca ggggctgacc
    22081 cgcttcatcg cggtctgcct gctgtcgggc tatgtctggc tggccttcgg tgcgctcgcc
    22141 gcgctcggcg gcgggctcgc gcccgcgcat ccactgcacg acatcaccat gcacgcgatc
    22201 gcgctcggct tcgtgttctc gatggtgttc ggtcacgccg cgatcatctt cccggcggtg
    22261 ctgaaggtga agatccccta ccacccggcc ttctacgtgc cgctcgcgct gctgcacgcc
    22321 tcgctcgcgc tgcgcgtcgc aggcagtctg gccgggcttc ctgcgctgcg cagctggggc
    22381 gggatcgcga atgcgctgac cctcgcggtc ttcatcgcca ccatggtcgt cagcgtgatc
    22441 cgcggcggac gcccgccggc acgccggcgc cggaaccccg tcccgggcac accggtccga
    22501 tccgctgtca aaccttccgg gaaccccgca ccatgaaccg caccctccgc tcccccctct
    22561 tccgcaagct cggtcgctgc ctgtccgcgc tcgcctgcgg cgccgcgctt ggcctcgccg
    22621 cgcccgccct cgccagcccc gacgaagtcg tgatgtacaa ggatcccaac tgcggctgct
    22681 gcggcaaatg ggccgagcac atgcgcagtg ccggcttcgc cgtgaaggag gtcgccagcc
    22741 cgcgcatgga tctcgtcaag caggaagccg gcgtgcccga ggccctgggc tcctgccaca
    22801 ccgcgaaggt cggcggctat gtggtcgagg gccacgtgcc ggcggccgac gtgcgccgca
    22861 tgctggcgga gaagccggcg atccgcggcc tctccgcacc cggcatgcca ctcggctcgc
    22921 ccggcatgga aggtccctac ccggccgaac gctacgacgt ggtgagcttc gacgcgcagg
    22981 ggcggagcgc agtgttctcg aagcactagg caggcggccg cgccgcgcgc cgctcttcgc
    23041 gacgcacgaa ggcgagcttg gcctcgcggg cgcgcagctg cccgcagccg ccgtccacgt
    23101 cctgcccggc ggactgcctc agcttggtga ggatgccggc gcggtgcagg cggcgggcga
    23161 tctccgccgc actctccgcc gccggacgac ggtagtcgag gccgtcgacg cggttgaacg
    23221 ggatcatgtt catcaccgcg tacttgccgg cgagcaggcg cacgatgccc tcgagctcgt
    23281 cctcgccgtc attcacgccc tcgatcagcg tccattggta ctggatcgga tagccgctcg
    23341 cgcgcgccca ggcctcgccg agctcgacca gctcggccgg gtcgatgcgc ggggcgcgcg
    23401 gcagcagcgc ggcgcgcagc gcggcgcgcg tgctgtgcag cgagagcgcc agcgccggct
    23461 tgacccgcat ctgcggcaga cgctcgaaga cgcggaggtc gcccacggtc gacagcacca
    23521 ggttcttgtg cggcagcgcg ccctcggtgc cgagcagctc gatcgcctcg atcaccgcat
    23581 cgaggttgtg cgcgggctcg cccatgccca tgaacaccac cttgtgcacc cggcgcaggc
    23641 ggcgcgcgag tgcgacctgg gcgacgattt cgccgctgtc gagctggcgc agcaggccgt
    23701 ccttgccggt catgcagaac acgcagccca ccgcgcagcc gacctgggtg gacacgcaca
    23761 ggccgtcgcg cggcagcagc acgctctcca ccgcctggcc gtcggcgagc tcgaccagca
    23821 ggcgggtgcc atcctcgccg gggtgctcgg agcgcacgcg ggccagcgct ccgagctccg
    23881 cttcgagctc gggcagcgcg gcctgcaagg ccttgggcag gcggttggat tcggtgcgcg
    23941 gaccggcgtc gagggcgtgt ccctgcagcc aggcgcgcag caggcgggcc tcgtggctgg
    24001 ggcgggcgcc gagcgcgcgc aggcgcaggc ggagctgttc gatttccatg gcgcgcgatg
    24061 ctagcacccg aaaagggcga gagcatccgc gatcgacacc ggtccggctg cccgcctgcc
    24121 atgcccggcc ctataatcgc ccgcatccga cacgaggaat gccgcatgac cgcactacgc
    24181 ttcgaaggcg cccaggacta cgtcgccacc cccgacctga tgctcgcggt caacgccgcg
    24241 atccgcctgc agcgcccgct gctgatcaag ggcgagcccg gcaccggcaa gaccatgctc
    24301 gccgagcagg tcgccgccgc gctcggactg ccgctgctgc agtggcacat caagtcgacc
    24361 accaaggcgc agcagggcct ctacga
//
LOCUS       DAOQBS010000036        24346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1318_length_24346_cov_14.859084, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000036 DAOQBS010000000
VERSION     DAOQBS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 24346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24346
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1318_length_24346_cov_14.859084"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1895)
                     /locus_tag="PKN25_06100"
     CDS             complement(3..1895)
                     /locus_tag="PKN25_06100"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HNV90507.1"
                     /translation="MTDYQQFHRRSIEDRDGFWAEQAQLVHWNKPAEKICDYSKPPFV
                     KWFKGGETNLCYNAVDRHAAARPNDRALVYISTETDEEKVYSFAELQREVERMAAIYQ
                     ELGVKRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFAAGSLATRIDDAKPVLM
                     VSSDAGMRNGKPVPYKHLVDEACKLAEFPPAKVLIVDRGLDKGFPKVEGRDVDYAELR
                     ARHLDAKVPVVWLESSEPSYILYTSGTTGKPKGVQRDTGGYTVALTASMKHIFTGGAG
                     ETMFSTSDIGWVVGHSYIIYGPLLAGMATIMYEGTPLRPDAGIWWQIVEKYKVTVMFS
                     APTAVRVLKKQDPAFLKKYDLSSLKHLFLAGEPLDETSHQWIMEELGIPVIDNYWQTE
                     TGWPMLAICRGVEDSPIKLGSPAFPVYGYDLRIFREDGTECGANEKGIVGIVPPLPPG
                     CLSTVWGQDERFVSTYFSLFTEPVVYSSSDWGIKDDKGYYTILGRMDDVINVAGHRLG
                     TREIEEAVQTHPAIAEVAVVGVADELKGQMPMAFAVVKNAADVDTPEKRAVLEKEVMK
                     KVDESLGAIARPARVHFIGGLPKTRSGKMLRRSIQALAEGRDAGDLTTIDDPSTLEQI
                     KAALKS"
     gene            complement(2032..3207)
                     /locus_tag="PKN25_06105"
     CDS             complement(2032..3207)
                     /locus_tag="PKN25_06105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV90508.1"
                     /translation="MDPGALTTDGDGRLPRILVVDDSRMVRASIVKHIRGRFEAREEP
                     DGEAAWEALLVDPAIEIVLTDIGMPRLDGYGLLGRLRASKLPRVRDLPVVIISGDEDE
                     SARARALALGANGFVTKGTGGVELLATLMSLLRLSKAQGELERSRAALERQSPVDPES
                     GLATEAYLEHHGAQAVAEARRRQADIGAMVIEIDQFDELATRYGVQVVQLILRKLGKL
                     VSGRIRAEDTLSQHGQACFALLSPGIDRVSTCAFALRLRASVEKLVMAYRDERIRVTL
                     SIGVASAVTDGREAIGALLALAQERATQAHTAGGNRVVAEGGEVDPAAIERELARVPS
                     VDGALRLLRAGARDEVAGQLPELVGALLPLLELLESRLQSGLPLARLREFHDKGGGG"
     gene            complement(3221..4216)
                     /locus_tag="PKN25_06110"
     CDS             complement(3221..4216)
                     /locus_tag="PKN25_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNV90509.1"
                     /translation="MNPFKAFVIDQDENRKVVGGMRTMSADQLDAGEVTIRVHYSSIN
                     YKDALAATGAGKIIRRFPCVGGIDLCGEVVDSADARFRPGDKVIATSFDIGVAHHGGY
                     AEYARVPAGWVVPLPAGLDLFEAMALGTAGFTAALGIVRMEDNGLAPANGPVVVTGAT
                     GGVGALAIDMLAQLGYHVVALTGKDAEADYLKMLGAAEILPRSSIDFDKVRPLEAARW
                     AGAVDNVGGQILHWVLATMKQAGTVASIGNAASFNVDTTVFPLILRGVSLLGVDSGYM
                     GFPTRQRVWERLATDLKPRHLAAITRTIDFDALPGAFDDFIHGRVKGRTVVRIGA"
     gene            4356..>4997
                     /locus_tag="PKN25_06115"
     CDS             4356..>4997
                     /locus_tag="PKN25_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV90510.1"
                     /translation="MRRTTATPTAPETANMPLLPSPLPSSAAYIDAFTRAGLPVLRHT
                     VRAFEELRSADPAPGAREIAAVVLADPLMTLRLLTHLEANRRASQNHDITTIERAVLM
                     LGVEPFFATFSDLPTVEDTLAAHPKALVVALKLIARARRAATLAREFATMRRDHEVQE
                     ITVATLLREATEIVCAIHAPTLTLEAIARRQAEPARSAAEVQREVFGISAAELQ"
     assembly_gap    4998..5097
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5196..6413)
                     /locus_tag="PKN25_06120"
     CDS             complement(5196..6413)
                     /locus_tag="PKN25_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV90511.1"
                     /translation="MLSSSSSLPAFLRRPAVVMACGALILTLAMGVRHTGGLFLQPMT
                     LDHGWSRELFSFSIALQNLLWGVFQPFAGAFADRHGAGRTLVGGALLYILGLVIMAHA
                     DSALGLNLGAGLLIGMGLSGTTFSVVLGVIGRMAAPEKRSLALGIASAGGSFGQFAVL
                     PVGQGLITAFGWQDALLWMAVGIAFIIPLAAAVTGTSERGGGVEQSLRQALAEAMRTP
                     SFHFLFWSFFVCGFQTAFVMLHLPAYVVDMGLSANIGMSAVAMIGLFNIFGSFLSGWL
                     GGLYSKKWLLAWIYALRIVAILALMLFPLSPLTLYVFAAVMGLLWLGTVPLTSGLVGH
                     IFGLRYVGMLYGIVFLGHQIGGFLGAWLGGRIFDLSGSYEMAWWLSIALSVMAAALSL
                     PVREAPLARLAAR"
     gene            6524..7099
                     /locus_tag="PKN25_06125"
     CDS             6524..7099
                     /locus_tag="PKN25_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNV90512.1"
                     /translation="MSIGSAVLLGLVVLTLVYGVVIYNGLVQLKHNLAKAWANIDVLL
                     KQRHDELPKLVEVCRQYKQFEQDTLARVTEARARVAQARETHDVPALGAAEQLLRSGL
                     GQIFAVAEAYPELRANEHFMQLQSRITALENAIADRREWYNEAVNVHNVRIEQFPDLL
                     IARPLGYSEQPLLQFSIAEKADVDLKALFGS"
     gene            7242..8144
                     /locus_tag="PKN25_06130"
     CDS             7242..8144
                     /locus_tag="PKN25_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90513.1"
                     /translation="MLVHLRHLRADLALPAIQLGAIALALRLGPGPGSLAALAVLLGT
                     GLLGTLRALRHARLIADTPTARIVSAAQGYTELRGRGAPLDGDPLLSPVNALPVLWYR
                     LRIERRQRNGEWRVVSTDTSASSFLLDDGSARCVVDPEGAEMLVRRRDVFVRDDLRYT
                     QWSLIEHDKLYVIGDFATLGSADVRTDTTTEVRELLTAWKADRPALLQRFDLDGDGEI
                     DLREWELARAQARREVRQRQAEALAAPELHLMRPPSGGRLYLISDLDPERIGRQYRWI
                     AAFHAAVFLGASAATAWFGQIGVF"
     assembly_gap    8293..8392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8430..10364)
                     /locus_tag="PKN25_06135"
     CDS             complement(8430..10364)
                     /locus_tag="PKN25_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV90514.1"
                     /translation="MPLLAVDNACLAFGHVDLLDHVSFQLDAGERVALIGRNGSGKSS
                     LLRALAGQAALDDGTVWRQAEMVTAYVPQEPDFELGRDVFETIADGLGAAARLLVDYH
                     AAMLEVAEHASPEAMARLDGLQHAVEAAGGWRLNQRVEQVVARLGLDPAAKVSSLSGG
                     GVKRVALARALVAEPDLLLLDEPTNHLDLDGILWLEGLIRDFRGAVIVITHDRVFLDN
                     VATRIIELDRGRLASYPGRFSDYQRRKAEELEAEEKAAARFDKFLAQEEVWIRKGVEA
                     RRTRNEGRVRRLEALRNARAARRDRLGDVSLAVDTGDKSGQMVAELTDVSKRYGDKVV
                     VRDFSTRILRGDRIGFIGPNGAGKTTLLKLILGEVEPDAGTVRRGTRQTVAYFDQLRA
                     QLDPELPLTEVISPGSDFVEIGGEKKHVIGYLGDFLFAPQRARSPVKSLSGGERNRLL
                     LARLFARPANVLVLDEPTNDLDIETLDLLEELLAGYDGTLFLVSHDRAFLDNVVTQVI
                     AAEGDGRWSEYAGGYGDWQRVQARRAEEAAAREATARESARRSAPPADKARAAEPKAA
                     NRPTKLSFNEKRELEALPERIAALEDEQTALHARMADPALYQNAPQEVAQIKARMEAL
                     DGEIETAMLRWEELESRAAG"
     gene            10546..12585
                     /locus_tag="PKN25_06140"
     CDS             10546..12585
                     /locus_tag="PKN25_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HNV90515.1"
                     /translation="MEKLLERFGQPDQDPVRLWSALVDKLRPARPTDTDQATGNLREL
                     GNLLARRPDLLANLRTAVLELFAERKQVTMYVSSGLLPSTGFFSETSRRFGSRMLPEL
                     LDTTHLKDLLSAVFHRVDDEVWVRAIADEAWQDFLRLLVGHQTPMFEEDASPLPSAVG
                     EILESLRVLSFHVSAIGLDRELVRIDPHLEEHESPFLAQNAELLAYIGHYKAWWTTPG
                     ALIADDKHLTVMLHQCDEVLQRVRKRAMRVGTSLTLTFKLERLRQHLERIHELIALLG
                     ELRIRRVVEDAAPRIVRLFKTLVRAECRKNILSDYWGQNVELLSLRMTESASRTGEHY
                     ITSSRSEYFGLFASAALGGLIIAFMAGNKVVLGSQGMAPLNELLSFCLNYGLGFALIH
                     MLGGTVATKQPAMTANAIAASIGEARGKTRDLEALADLIVRTIRSQIGAILGNIGVAI
                     PVAIGVGVLIHLATGSHFISPEKAHSLLAEIHPLGGALFFAAIAGVCLFMSGLIAAYY
                     DNLSAYNRIPQRLTQLRGLRRLLGERRLRKLADYVEQNIGALVGNFFFGFLLGGATGL
                     GVLFGLPIDIRHIAFSSAYLGYAAAALDFSIPLTTAAIAFAGVLLIGLTNLTVSFALT
                     LSVAMRARRITFAQSRSLGGLLLRRLLRTPHAFLFPPRTDQGVDQSALGPTPPNG"
     gene            12604..12678
                     /locus_tag="PKN25_06145"
     tRNA            12604..12678
                     /locus_tag="PKN25_06145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12637..12639,aa:Arg,seq:cct)
     gene            12778..13437
                     /locus_tag="PKN25_06150"
     CDS             12778..13437
                     /locus_tag="PKN25_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNV90516.1"
                     /translation="MPRYSQVLFDLDGTLIDSAPAILASYRDAFASTGHQPVIAIDAG
                     IVGPPLLETLRMLAGTTDIGVIDALAAGFKASYDSRGYRQTAAYAGVDAMLRRLGAAG
                     CTLSIATNKRLLPTRLILEHLGWSGYFAAVYALDLFEPRLPDKASMIARLLQDRGIAR
                     GDAVYVGDRSEDGESADANRLPFIAATWGYGSLESTEMAPHWRAVASPAALVDAILGD
                     A"
     gene            complement(13463..14260)
                     /locus_tag="PKN25_06155"
     CDS             complement(13463..14260)
                     /locus_tag="PKN25_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNV90517.1"
                     /translation="MNPRPETRARRRLLACALALPAALALAPIARAELASTLARVKPS
                     VVAVGTYQRTRSPAFQFRGTGFAVGDGMLVATNAHVLPDEIDGAKMEALVIVLPGDDL
                     TGAVRPVKVVATERAQDLALLRLEGGPPLPALRLAGGGGVVEGQEVAFTGFPIGAVLG
                     MTPVTHRGIVSAITPIAIPQANSRDLNPALIRRLATDPFRVYQLDATAYPGNSGSPLY
                     DPASGEVIGVLNMVFVKSTKEKVLADPSGISYAIPVEYLQRLIAGRN"
     gene            complement(14257..15222)
                     /locus_tag="PKN25_06160"
     CDS             complement(14257..15222)
                     /locus_tag="PKN25_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNV90518.1"
                     /translation="MDKTSCPVPVGRIARPRLRRLPRLLGLAVALASTVCAAAGEGAF
                     ERALAEQARYLATEALAYEHGEGVPRDQSYAALLYCESARLGDSEGMYALGWMYANGR
                     GVERNDAYAGTLFAMAAAKGDEHAQRMLRYTGEYTGDVPDCLHTPSTQIVQRWPVEAL
                     IARLPAVRQAVARMLAELAPKYGVRPEFALAIGLTESALNPDALSPKNAMGVMQLIPA
                     TAERFNVGKPYDPEQNIRGGLAYLRWLLAYFEGDIALAAAAYNAGEGAVDRYRGVPPY
                     RETRAYVERILRHVGQDRHPYDSRVVPPSPMLRKLRLALEEEHES"
     gene            complement(15274..18078)
                     /gene="prsT"
                     /locus_tag="PKN25_06165"
     CDS             complement(15274..18078)
                     /gene="prsT"
                     /locus_tag="PKN25_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNV90519.1"
                     /translation="MSENRTVRHHPRWSRPIRGIAVAAFSALVLVGCGETPEQMLASA
                     KSFLEKQDFDAAGIQLKNALQENANLAEARFLLGRVNLRQGNVAGAVKELERALELGY
                     PRDAVAAELAPALVRAGEFDRLLAEFGDPRVTAPATIAIVQAALGDAHLARREIDKAQ
                     AAYLQALGANADEHGARIGFARTAAIKGDLKAAEEAVREVIARAPQVADAHALLGDLL
                     VARGDLSQGLAALREAVRLQPGTVANHYALVTLLLRQNAIAEADAALDAMKSAVGRHP
                     FTRYLLALRDYRENRLVEARDALMQVLKESPGLLVAELLAGVVHLRLNDHEIARAHLD
                     RVLQAAPGNLVARQALVASHLATGESKRALELVQPALQVPQPSARLLGLAGQVYIANG
                     DFERAEDYFERAAKAAPADVRARTRLGVARMAVGDADAGFTELEEASRMDEGGIQADL
                     ALVVGHLRRGDAKKALAAQAELERKQPDNALVHNLRGGLMLATRNIPAARAAFEKALA
                     KQPTYLSAAVSLARLDLAERRPEMAIGRIKAVVDRDPRSVEALLTFAEMQRLTGAAPI
                     EVLGTLERAEAVSPGAPVVGLATIQHHLSEGQPAEALRVAQKLATAHPNDPRAIEALA
                     RAQFAAGDSQQAISALNRLVGLLPESPLPLVALADMHRALMDGQAAEQALRKALGIAP
                     DSMDVRQRLIALALQRKDPQGALRLAREAQSRQPELPAGYLLEGDVHAVGSQWPDAAE
                     AYRKALERKGGGTAAVRLHSALLRADRRADANRMSEGWLKANPTDLVLRGHLGELALS
                     EKRYADAARIFARMAEMAPQNPIILNNLAWAANEAKDPKALEYAEQALRLAPDNPAII
                     DTVGTIQVASGAVDAGLANLRRAVSIGPDLLPLQLNLVKALAKAGRKDEARSQLDALM
                     PRLQEGTPMHQEARALQASL"
     gene            complement(18075..19448)
                     /gene="prsR"
                     /locus_tag="PKN25_06170"
     CDS             complement(18075..19448)
                     /gene="prsR"
                     /locus_tag="PKN25_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HNV90520.1"
                     /translation="MNSTQDKQRTLLIVEDDLALQKQMRWAFDASETVVASDRESAIA
                     QLRKHEPAVVTMDLGLPPAPDDVSEGFKLLREILTLAPDTKVIVLTGQHDRENAIKAV
                     GLGAYDFFAKPFEPELLNLTIERAFRMHDLQRENARLAALEGSPLSGLLTRDPGMLKV
                     CRTIEKLASASATVALLGESGTGKEILARGLHMRSARARERFVAINCAAIPDALLESE
                     LFGYEKGAFTGAVKQTLGKIETANGGTLFLDEIGDLPLALQAKLLRFLQERSIERIGG
                     REEIPVDVRIVCATHRNLKAQIQAGVFREDLYYRLAEIVIEIPPLRERDGDATYLAHA
                     FAQRFAKENGRRTPIFTDDALSAIEAHRWPGNVRELENCIKRAVIMADGECIAAEDLG
                     LAAGDEDLSIFNLRQVREDAERAAVLKVIARTNGNIARAAEILGVSRPSLYDLLSRFG
                     LKKENAS"
     gene            complement(19452..21521)
                     /gene="prsK"
                     /locus_tag="PKN25_06175"
     CDS             complement(19452..21521)
                     /gene="prsK"
                     /locus_tag="PKN25_06175"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HNV90521.1"
                     /translation="MLEVAIWSYGVAAVAFLIFALYIFLAWRGALPGGALFAAVAISG
                     LWAGLSALEARGAWPFSGALAVALDLLRGAAWYVFLIVLSRPLWGRWMRWPAYVALAV
                     VVAQVLALALESAGVTGGLPIAPAIGAWLMHAIVGLMLVEQLYRGMPAVSRWGLKPLC
                     LALAAGYIFELYLFADALLFSRIDADVWAARGIAHALLIPLIAIAGTRSPSWTLRMSV
                     SREIVFHSTALAGAGLYLLVIAAAAYYVRFFGGDWGRALQMALLFAGLVVLGALLFSG
                     AVRARLRVFISKHLFPYRYDYRSEWLRFTQALSTVDRSLDLGQSVIKALSDLVESPGG
                     SLWLADAYGKCRPHSRFNQPPIDVVEALDSPLCGFLARSGWVINLEEYRVQPGRYQGL
                     VLPDWLSRLDEAWLVIPLQTAERLIGFVVLGSPRTPFDIDWEVLDLLKTAQRQAAGYL
                     DRMLAAEALLEARKFDSFNRMSAFVVHDLKNLVAQLSLMLKNAQRHRDNPEFQQDMLD
                     TVANVEARMRGLMTQLQEKRSIDPPRAVDLPALLRAVCESKRGQSPAIELVCAAAGPV
                     EVSAHPQRLERIIGHVVQNALDATAQDGKVVLSLAAGKDGRANVVVEDDGCGMSPEFV
                     RERLSRPFQTTKSSGMGIGVYETRQYLEEIGGALHYDSEVGRGTRVSIELPRIRREDG
                     QSTAMGI"
     gene            complement(21600..22985)
                     /locus_tag="PKN25_06180"
     CDS             complement(21600..22985)
                     /locus_tag="PKN25_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNV90522.1"
                     /translation="MLKVFSHYLPTHTLQQVLFDALTLFAVVLAAVAVLVTPTSAVDW
                     AICIPSALTFAASMIALNAAMGLYRPVYQRSHRQTFARVALSLTVSVPVAYLVFGLLP
                     WSEIAPHSLQLSVLLLLCSLLLVRGLVNQRQASALFVPRVLIVGTGRDARMVQQDLVR
                     PLQRSVEVVGFLPVGGNERVEVEQHAVLPPGSLLEVVRNLRVDEVIVAVRERRGGVLS
                     LRELLDCKLLGTRILDLSTFFERVQGQVRLDSLRASWLIYGDGFRQGWTRTFVKRCFD
                     LVVGTALLLVALPIMVLTALLILLEDGAPIFYSQERVGRGGKPFRVIKFRSMRRDAEK
                     DGKPRWATSNDDRVTRVGRFIRKLRIDELPQLFNVLKGDMSLVGPRPERPYFVDQLTQ
                     QIPFYAVRHCVKPGVTGWAQVRYQYGASVDDAAEKLQYDLYYVKNHSLILDTLVLFET
                     VRVVLTGEGAH"
     gene            23290..23739
                     /locus_tag="PKN25_06185"
     CDS             23290..23739
                     /locus_tag="PKN25_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNV90523.1"
                     /translation="MTQAQRDFSEVYGLSFEDLHIGRTASVSRTVSEADILMFAGVSG
                     DTNPVHLDEEFAASTMFGGRIAHGMLSAGLISAVFGTRLPGPGCIYLSQSLKFKAPVK
                     IGDTVVARVTVKELKAEKRRAVFSTVCTVGDKVVMDGEAEILVPART"
     gene            23895..24332
                     /locus_tag="PKN25_06190"
     CDS             23895..24332
                     /locus_tag="PKN25_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV90524.1"
                     /translation="MDVVIPRRSRRTHSEAFKQSVVSACREPGVSVAGLALANGLNAN
                     RVHRWMRERGIEPPSPRKPLCAERPPLAEPGFVAVQVAPAVETLAIRLEAQRGNARIE
                     LEWPLQAAARRPRSTGCRCLAPPAHRSGARARVAPDQDRAAHA"
BASE COUNT         3888 a         8436 c         8156 g         3666 t
ORIGIN      
        1 gctcagctct tcaaggccgc cttgatctgc tcgagcgtgc tcggatcgtc gatcgtggtc
       61 aggtcgccgg cgtcgcgccc ctcggccagc gcctggatcg agcgccgcag catcttgccc
      121 gagcgcgtct tgggcaggcc gccgatgaag tgcacccgag ccggacgcgc gatcgcgccg
      181 aggctctcgt cgaccttctt catgacctcc ttctcgagca cggcgcgctt ctcgggggtg
      241 tcgacgtcgg cggcgttctt gaccaccgcg aaggccatcg gcatctggcc cttcaactcg
      301 tcggccacgc ccaccaccgc cacctcggcg atcgccgggt gggtctgcac cgcctcctcg
      361 atctcgcgcg tgccgagacg gtggccggcg acgttgatca cgtcgtccat gcggccgagg
      421 atggtgtagt agcccttgtc gtccttgatg ccccagtcgg acgaggaata caccaccggc
      481 tcggtgaaca ggctgaagta ggtgctgacg aagcgctcgt cctggcccca cacggtggac
      541 aggcagcccg gcggcagcgg cggcacgatg ccgacgatgc ctttctcgtt ggcgccgcac
      601 tcggtgccgt cctcgcggaa gatgcgcagg tcgtagccat agaccgggaa ggcgggcgag
      661 ccgagcttga tcgggctgtc ttccacgccg cggcagatgg cgagcatcgg ccagccggtc
      721 tcggtctgcc agtagttgtc gatgaccggg atgccgagct cttccatgat ccactggtgg
      781 ctggtctcgt cgagcggctc gccggcgagg aacaggtgct tgagcgagga caggtcgtac
      841 ttcttgagga aggccgggtc ctgcttcttg agcacgcgca ccgcggtggg cgcggagaac
      901 atcaccgtga ccttgtactt ctcgacgatc tgccaccaga tgccggcgtc ggggcgcagc
      961 ggcgtgccct cgtacatgat cgtggccatg cccgcgagca gcgggccgta gatgatgtag
     1021 ctgtggccga ccacccagcc gatgtccgag gtcgagaaca tggtctcgcc cgcgccgccg
     1081 gtgaagatgt gcttcatcga cgcggtcagg gccacggtgt agccgccggt gtcgcgctgc
     1141 acgcccttgg gcttgccggt ggtgccggag gtgtacagga tgtagctcgg ctcggaggac
     1201 tcgagccaca cgaccgggac cttggcgtcc aggtgcctgg cacgcagctc ggcgtagtcg
     1261 acgtcacggc cttccacctt ggggaagccc ttgtccaggc cgcgatcgac gatcagcacc
     1321 ttggcgggcg ggaattcggc gagcttgcag gcttcatcca ccaggtgctt gtagggcacc
     1381 ggcttgccgt tgcgcatgcc ggcatccgag ctgaccatca gcaccggctt ggcgtcgtcg
     1441 atgcgggtgg cgagcgagcc ggccgcgaaa ccgccgaaca ccaccgagtg gatcgcgccg
     1501 atgcgcgcgc aggccagcat ggcaaacgcg gcctcggcga tcatcggcat gtagatcagc
     1561 acgcgatcgc cgcgcttgac gccgagctcc tggtagatcg ccgccatgcg ctcgacctcg
     1621 cgctgcagct cggcgaagga atagaccttc tcctcgtcgg tctcggtcga gatatacacc
     1681 agcgcacggt cgttcgggcg cgcggcggcg tggcgatcga cggcgttgta gcacaggttg
     1741 gtctcgccac ccttgaacca cttgacgaac ggcggcttgg aatagtcgca gatcttctct
     1801 gcgggcttgt tccagtgcac gagctgcgcc tgctcggccc aaaagccgtc gcggtcttcg
     1861 atggaacggc ggtggaactg ctggtaatcg gtcattgctt cctctccctt ctgccttcaa
     1921 tgatcgtgtt gtcgaactcg acgtgccacc ggcagcgcgg cgccgggaca gcgcagcgaa
     1981 gcccgcgggc acacctgttg gggacggcct gcgggtgcgg ccgccctcca ctcatccgcc
     2041 cccgcccttg tcgtggaact cgcggagtct tgcgagcggt aatccggatt gaagcctgga
     2101 ttctagcaat tcgagcagcg gcagcagcgc cccgacgagc tcgggcaact gcccggccac
     2161 ctcgtcacgc gcgccggcgc gcagcaggcg cagcgcgcca tcgacgctgg ggacgcgcgc
     2221 aagctcgcgt tcgatcgccg ccgggtcgac ctcgccgccc tcggcgacga cgcggttgcc
     2281 acccgccgtg tgcgcctgcg tggcgcgctc ctgggcgagc gcgagcagcg cgccgatggc
     2341 ctcgcgaccg tcggtgaccg cgctggcgac gccgatgctc agggtcacgc ggatgcgctc
     2401 gtcgcggtag gccatcacca gcttctcgac gctggcgcgc aggcgcagcg cgaaggcgca
     2461 ggtggacacg cggtcgatgc ccggcgacag cagcgcgaag caggcctggc catgctgcga
     2521 caaggtgtcc tcggcgcgga tgcgcccgct caccagcttg ccgagcttgc gcaggatgag
     2581 ctgcaccacc tgcacgccgt agcgcgtggc gagctcgtcg aactggtcga tctcgatcac
     2641 catcgcgccg atgtcggcct ggcgccggcg cgcctcggcc acggcctggg cgccgtgatg
     2701 ctcgaggtag gcctcggtcg ccaggcccga ctcggggtcg accgggctct ggcgctcgag
     2761 cgcggcgcgg ctgcgctcga gttcgccctg cgccttggac aggcgcagca gcgacatcag
     2821 cgtggcgagc agctcgactc cgcccgtgcc cttggtgacg aagccgttgg cgccgagggc
     2881 gagcgcgcgc gcgcgcgcgg actcgtcctc gtcgccggag atgatcacca ccggcaggtc
     2941 gcgcacccgc ggcagcttcg aggcacgcag gcgcccgagc aggccgtagc cgtccaggcg
     3001 cggcatgccg atgtcggtga gcacgatctc gatcgccgga tcgaccagca gcgcctccca
     3061 ggcggcctcg ccgtcgggct cctcgcgcgc ctcgaaacga ccgcggatgt gcttgacgat
     3121 ggacgcgcgc accatgcgcg agtcgtccac gacgaggatg cgcggcagcc tgccgtcccc
     3181 gtccgtcgtc agcgcgcccg ggtccatggc gttcttcggc tcaggcgccg atgcgcacga
     3241 cggtgcgccc cttgacgcgg ccgtggatga agtcgtcgaa ggcgcccggc aacgcgtcga
     3301 agtcgatcgt gcgcgtgatc gcggcgaggt ggcgcggctt gaggtcggtg gccaggcgct
     3361 cccaaacgcg ctgccgggtc gggaagccca tgtagcccga atccaccccg agcaggctca
     3421 ccccgcgcag gatgagcggg aacaccgtgg tatcgacgtt gaagctcgcc gcgttgccga
     3481 tgctcgccac cgtgccggcc tgcttcatgg tcgccagcac ccagtgcagg atctgcccgc
     3541 cgacgttatc caccgcgccg gcccagcgcg ccgcctcgag cggtcgcacc ttgtcgaagt
     3601 cgatggagga gcgcggcagg atctccgccg cgccgagcat cttcaggtag tcggcctcgg
     3661 catccttgcc ggtgagcgcg accacgtggt aaccgagctg cgccagcatg tcgatggcca
     3721 gcgcgccgac gccacccgtg gcgccggtga ccaccaccgg cccgttggcc ggggcgaggc
     3781 cgttgtcctc catgcgcacg atgccgagcg cggcggtgaa gcccgcggtg cccagcgcca
     3841 tcgcctcgaa gagatcgagc cccgccggca agggcacgac ccagcccgcc ggcacgcgcg
     3901 cgtactcggc atagccgcca tggtgggcga ccccgatgtc gaagctggtg gcgatcacct
     3961 tgtcgcccgg cctgaagcgc gcatcggcgc tgtcgaccac ttcgccgcac aggtcgatgc
     4021 cgccgacgca ggggaagcga cggatgatct tgccggcgcc ggtcgcagcg agcgcgtcct
     4081 tgtagttgat gctggagtag tggacgcgga tcgtcacctc gcccgcatcg agctggtcgg
     4141 cgctcatcgt gcgcatgccg ccgaccacct tgcggttctc gtcctggtcg atcacgaagg
     4201 ccttgaaggg attcatgcgg aagtctcctg tcgatgtggc ggccagcctg gaggctgcgt
     4261 tgattcataa ttatagccgc aactgcgaat ctgcccgaac acggcgcgct cgcctcaagt
     4321 ttgtgccgcg gcggccgtca tcccggtttg attccatgcg ccgcaccacc gccaccccga
     4381 ccgcgccaga gaccgcgaac atgcccctgc tgcccagccc cctgccctcc tccgcagcct
     4441 acatcgacgc cttcacccgc gccggactgc ccgtcctgcg ccacaccgta cgcgccttcg
     4501 aggaactgcg cagcgcggat cctgcgccgg gcgcacgcga gatcgccgcg gtggtgctgg
     4561 ccgatccgct gatgaccttg cgcctgctca cgcatctcga ggcgaaccgg cgcgcctcgc
     4621 agaaccacga catcaccacc atcgagcgcg cggtgctgat gctgggcgtg gagccgttct
     4681 tcgccacctt ctccgatctt cccacggtgg aagacaccct cgcggcgcac ccgaaggcgc
     4741 tcgtcgtggc gctcaagctg atcgcgcgcg cacgccgggc agccacgctc gcgcgcgagt
     4801 tcgcgacgat gcggcgcgat cacgaggtgc aggagatcac cgtcgccacc ctgctgcgcg
     4861 aggccacgga gatcgtgtgc gcgatccacg cccccacgct caccctggag gcgatcgcac
     4921 gccggcaggc cgaacccgcg cgctcggcgg cggaggtgca gcgcgaggtg ttcggcatca
     4981 gcgcagcgga gctccagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa
     5101 atcgagcagc agcgccggct gctggtaggc ggcgaagagc aggccgagcg cgagcgcccc
     5161 cagcacgacg agcgccagcc gcagcaggcc cttcctcatc gcgccgccag ccttgcgagc
     5221 ggcgcctcgc gcaccggcag cgacagcgcc gccgccatca ccgagagcgc gatcgacagc
     5281 caccacgcca tctcgtacga gccgctgaga tcgaagatgc gcccgcccag ccaggccccc
     5341 aggaagccgc cgatctggtg gccgaggaag acgatgccgt acagcatgcc gacgtagcgc
     5401 aggccgaaga tgtggccgac caggccgctg gtgagcggca ccgtgcccag ccacagcagg
     5461 cccatcaccg cggcgaagac gtagagcgtg agcgggctga gcgggaacag catcagcgcc
     5521 aggatggcca cgatacgcaa cgcgtagatc cacgccagca gccacttctt gctgtagagg
     5581 ccgcccagcc agcccgacag gaaggagccg aagatgttga acagcccgat catcgccacc
     5641 gcgctcatgc cgatgttggc cgacagaccc atgtcgacca cgtaggcggg cagatgcagc
     5701 atgacgaagg cggtctggaa gccgcacacg aagaagctcc agaacaggaa gtggaagctc
     5761 ggcgtgcgca tcgcctcggc gagcgcctgg cgcagcgact gttcgacacc gccgccgcgc
     5821 tcgctggtgc cggtgaccgc ggcggcgagc gggatgatga aggcgatgcc caccgccatc
     5881 cacagcagcg cgtcctgcca gccgaaggcg gtgatcagcc cctgtcccac cggcagcacc
     5941 gcgaactggc cgaagctgcc gccggccgag gcgatgccga gcgcgaggct gcgcttctcc
     6001 ggcgcggcca tgcgcccgat cacgcccagc accacgctga aggtggtgcc cgagaggccc
     6061 atgccgatca gcaggccggc gccgagattg aggccgagcg cgctgtcggc gtgagccatg
     6121 atcaccaggc ccaggatgta gagcagcgcc ccgcccacca gggtgcggcc ggcgccgtgg
     6181 cggtcggcga aggcgccggc gaagggctgg aacacgcccc acagcaggtt ctgcagcgcg
     6241 atcgagaagg agaacagctc gcgcgaccag ccgtggtcga gcgtcatcgg ctgcaggaac
     6301 aggccgccgg tgtggcgcac gcccatggcc agcgtgagga tcagcgcgcc gcaggccatg
     6361 acgaccgcgg ggcggcgcag gaaggcggga agggaggaag acgaggagag caaaatggga
     6421 atttccgctg cgagggaggc gattcgccgc caccgtggca tgcgcaacac gtggcgcgag
     6481 ggcgcgagta taagatgcgc gcattcgcaa tggagtcccc gcaatgagca tcggcagcgc
     6541 agtcctcctc ggcctcgtcg tcctcacgct ggtgtacggc gtggtgatct acaacggcct
     6601 ggtgcagctc aagcacaacc tcgccaaggc ctgggccaac atcgacgtgc tgctcaagca
     6661 gcgccacgac gaactcccca agctggtcga ggtgtgccgc cagtacaagc agttcgagca
     6721 ggacacgctc gcgcgcgtga ccgaggcgcg cgcgcgcgtc gcccaggcgc gcgagaccca
     6781 cgacgtgccc gcgctcggtg ccgccgagca gctgttgcgc agcgggctgg ggcagatctt
     6841 cgcggtcgcc gaggcctacc ccgagctgcg cgccaacgag cacttcatgc agctgcagag
     6901 ccgcatcacc gcgctcgaga acgccatcgc cgaccgccgc gagtggtaca acgaggcggt
     6961 caatgtacac aacgtgcgca tcgagcagtt ccccgacctg ctgatcgcgc gcccgctcgg
     7021 ctacagcgag cagccgctgc tgcagttcag catcgctgag aaggcggatg tcgatctgaa
     7081 ggcgctgttc ggcagctgag cgcgcgtggc gagcgccgca cgatcggcgt cgcggacgaa
     7141 ccggaccggc gcggaaccgg ccccgggccc gtcggcgatc ttgccgcagc gcacgcaggc
     7201 ccgctcgccc cgccgcgatc gacaccaccc ggtaccccac catgctcgtg caccttcgcc
     7261 acctgcgggc agacctcgcg ttaccggcca tccagctcgg cgcgatcgca ctcgcactgc
     7321 gcctgggccc cggcccgggc agcctggccg cgctcgcggt gctgctcggc accggcctgc
     7381 tcggcactct gcgcgcgctg cgccacgcgc gcctgatcgc cgacaccccg accgcgcgca
     7441 tcgtctcggc ggcgcagggc tacaccgaac tgcggggccg cggcgcgccg ctggacggcg
     7501 atccgctgct gtcgccggtg aatgcgctgc cggtgctgtg gtaccgcctg cgcatcgagc
     7561 gccgccagcg caacggcgag tggcgggtgg tgagcacgga caccagcgcg tcctcctttc
     7621 tgctcgacga cggcagcgcg cgctgtgtgg tcgacccgga gggcgcggag atgctggtgc
     7681 ggcgacgcga cgtgttcgtg cgcgacgatc tgcgctacac gcaatggtct ctgatcgagc
     7741 acgacaagct ctatgtgatc ggcgacttcg ccaccctggg cagcgccgac gtgcgcaccg
     7801 acaccacgac cgaggtgcgc gagctgctca ccgcctggaa ggccgaccgc cccgccctgc
     7861 tgcagcgctt cgacctcgac ggcgacggcg agatcgacct gcgcgagtgg gagctcgccc
     7921 gcgcccaggc gcgtcgcgag gtgcgccagc gccaggccga ggcgctcgcc gcgccggagc
     7981 tgcacctgat gcgcccgccg agcggcggcc gcctgtacct gatctcggac ctcgaccccg
     8041 agcgcatcgg tcgccagtac cgctggatcg ccgccttcca cgccgcggtc ttcctcggcg
     8101 ccagcgcggc gacggcgtgg ttcgggcaga tcggcgtgtt ctagcgcggg ccgcagaagt
     8161 cgcggcgccc ttgcccggca cggtggacgg ttcgtcaccg tggatcgcga cttgcgtcgc
     8221 tcctacggcg cggctccgtc agcgcggcgg tcttcgtggc gccggtttcg taggagcgac
     8281 gcaagtcgcg atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacggcgcg
     8401 ggtcatccgc gccgcggccc gcctcgcgct caacctgccg cgcgcgactc gagctcttcc
     8461 cagcgcagca tcgcggtctc gatctcgccg tccagcgcct ccatccgcgc cttgatctgc
     8521 gcgacctcct gcggggcgtt ctggtacagc gccgggtcgg ccatgcgcgc gtgcaacgcg
     8581 gtctgctcgt cctcgagcgc ggcgatgcgc tcgggcaagg cctcgagctc gcgcttttcg
     8641 ttgaaggaca gcttggtcgg gcggttcgcg gccttgggct cggcggcgcg cgccttgtcc
     8701 gcgggcggag cgctgcgcct ggcgctctcg cgcgccgtcg cctcgcgcgc ggcagcctcc
     8761 tcggcacgcc gtgcctgaac gcgctgccag tcgccgtagc cgccggcgta ttcgctccaa
     8821 cgcccgtcgc cctcggcggc gatcacctgg gtgaccacgt tgtcgaggaa ggcgcggtcg
     8881 tggctgacga ggaagagcgt gccgtcgtag ccggcaagca gctcctccag caggtcgagg
     8941 gtctcgatgt cgaggtcgtt ggtgggctcg tcgagcacca gcacgttggc cgggcgggcg
     9001 aacagacgcg ccagcagcag gcggttgcgc tcgccgccgg agagcgactt gaccggtgag
     9061 cgcgcgcgct gcggcgcgaa caggaagtcg ccgaggtagc cgatgacgtg cttcttctcg
     9121 ccgccgatct cgacgaaatc cgagcccggg ctgatgacct cggtgagcgg cagctcgggg
     9181 tcgagctggg cgcgcagctg gtcgaagtag gccaccgtct gccgggtgcc gcggcgcacc
     9241 gtgcccgcgt cgggctcgac ctcgccgagg atgagcttca acagcgtggt cttgccggcg
     9301 ccgttggggc cgatgaagcc gatgcgatcg ccgcgcagga tgcgggtgga gaaatcgcgc
     9361 accaccacct tgtcgccgta gcgcttggag acgtcggtga gctccgcgac catctggccg
     9421 ctcttgtcgc cggtgtcgac cgccaggctg acgtcgccga ggcggtcgcg acgcgcggcg
     9481 cgcgcgttgc gcagggcctc gagccgacgc acgcggcctt cgttgcgggt gcgccgggcc
     9541 tccacgccct tgcggatcca cacctcctcc tgggcgagga acttgtcgaa gcgcgcggcg
     9601 gccttctcct cggcttcgag ctcttccgcc ttgcggcgct ggtagtcgga gaagcgcccc
     9661 gggtagctcg ccagccggcc gcggtcgagc tcgatgatgc gggtggcgac gttgtcgagg
     9721 aagacgcggt cgtgggtgat gacgatgacc gcgccgcgaa agtctcggat cagcccttcg
     9781 agccacagga tgccgtcgag gtcgaggtgg ttggtgggtt cgtcgagcag cagcaggtcg
     9841 ggctcggcga ccagcgcgcg cgccagcgcc acacgcttga cgccgccgcc ggagaggctg
     9901 gaaacctttg ccgccgggtc gagtccgagt cgggcgacca cctgctcgac gcgctggttg
     9961 agccgccagc cgcccgcggc ctcgaccgca tgctgcaggc cgtccagccg ggccatggcc
    10021 tcggggctgg cgtgctcggc gacctccagc atggcagcat ggtagtcgac gagcaggcgg
    10081 gcggcggcgc ccaggccgtc ggcgatggtc tcgaagacgt cgcgcccgag ttcgaagtcc
    10141 ggctcctgcg gcacgtatgc cgtgaccatt tcagcctgcc gccacactgt gccatcatcg
    10201 agcgccgcct ggccggcgag cgcgcgcaga aggctggatt tgcccgagcc gttgcgcccg
    10261 atgagcgcca cgcgctcccc ggcgtcgagc tggaagctca cgtgatcgag caggtcgacg
    10321 tgaccgaagg cgaggcaggc gttatctacg gcgaggagcg gcatcttggc gggcacggcg
    10381 cgcgggccgc gggtcggatc ggggtggcga ttctagcccc gcgctggcgg ctccggcgga
    10441 atcccgcata atgcagtgca caaaaccgcc gcgcggcgac ccggttcagc gcgcgacggt
    10501 cgcgcctggc gcggccgcga ggcggatacg gaacacggga aacgaatgga aaaactgctc
    10561 gaacgcttcg gccagccgga ccaggatccg gtccggctgt ggtccgcact ggtcgacaag
    10621 ctgcgccccg cgcgcccgac cgacaccgac caggccaccg ggaacctgcg cgaactcggc
    10681 aacctgctcg cgcgccggcc cgatctgctg gcgaacctgc gcacggcggt gctggagctc
    10741 ttcgccgagc gcaagcaggt gacgatgtac gtctcctccg gcctcctgcc ctcgaccggg
    10801 ttcttctccg agacctcgcg caggttcggc agccgcatgc tgcccgagct gctcgacacc
    10861 acccacctca aggacctcct ctccgcggtc ttccaccgcg tggatgacga agtctgggtg
    10921 cgcgccatcg cggacgaggc ctggcaggac ttcctccgcc tgctggtcgg acaccagacc
    10981 ccgatgttcg aggaagacgc cagcccgctg ccgagcgcgg tgggcgagat cctcgagtcg
    11041 ctgcgcgtgc tctccttcca cgtctcggcg atcgggctcg accgcgagct ggtgcgcatc
    11101 gatccccacc tcgaggagca cgaatccccc ttcctcgcgc agaacgccga gctgctcgcc
    11161 tacatcggcc actacaaggc gtggtggacc accccgggcg ccctgatcgc cgacgacaag
    11221 cacctcaccg tgatgctgca ccagtgcgac gaggtgctgc agcgggtgcg caagcgcgcg
    11281 atgcgggtgg gcaccagcct gacgctgacc ttcaagctcg agcgcctgcg ccagcacctg
    11341 gagcgcatcc acgagctcat cgcgctgctc ggcgagctgc gcatccggcg cgtggtcgag
    11401 gacgccgcgc cccgcatcgt gcgcttgttc aagaccctgg tgcgcgccga gtgccgcaag
    11461 aacatcctct ccgactactg gggccagaac gtcgagctgc tgtcgctgcg catgaccgag
    11521 agcgcgagcc gcaccggcga gcactacatc accagttcgc gcagcgagta tttcgggctg
    11581 ttcgcatcgg ccgcgctcgg cgggctgatc atcgccttca tggctggcaa caaggtcgtg
    11641 ctcggcagcc agggcatggc cccgctcaac gagctgctgt ccttctgcct caactacgga
    11701 ctgggcttcg cgctgatcca catgctcggc ggcacggtcg ccaccaagca gccggcgatg
    11761 accgcaaacg cgatcgccgc ctcgatcggc gaggccaggg gcaagacgcg cgacctggag
    11821 gcgctcgccg acctgatcgt gcgcacgatc cgcagccaga tcggtgccat cctcggcaac
    11881 atcggggtgg cgatcccggt ggcgatcggc gtgggcgtgc tgatccacct cgccaccggc
    11941 agccatttca tcagcccgga gaaagcgcac tcattgctgg ccgagatcca tccactcggc
    12001 ggcgcgctgt tcttcgccgc gatcgcgggc gtgtgcctgt tcatgtcggg gctgatcgcc
    12061 gcctattacg acaacctctc ggcctacaac cgcattccgc agcggctcac ccagctgcgc
    12121 ggcctgcgcc gcctgctcgg cgagcgccgc ctgcgcaagc tcgcggacta cgtcgagcag
    12181 aacatcggtg ccctcgtcgg caacttcttc ttcggtttcc tgctcggcgg cgccaccggc
    12241 ctgggggtac tgttcggcct gccgatcgac atccgccaca tcgccttctc gtcggcctac
    12301 ctcggctacg ccgcggcggc actcgacttc tcgatcccgc tgacgaccgc ggcgatcgcc
    12361 ttcgccggcg tgctgctgat cggcctcacc aacctgacgg tgagcttcgc gctgacgctg
    12421 agcgtggcga tgcgcgcacg ccgcatcacc ttcgcgcaga gccgctcgct cggcggcctg
    12481 ctgctgcgcc gcctgctgcg cacgccccat gccttcctgt tcccgccgcg cacagaccaa
    12541 ggcgtggatc aaagcgcgct tggtccgacg ccgccgaacg gctagaatgc gcgtccccga
    12601 gtcctcgtca tagttcaatg gatagaacgg gcgcctccta agcgccagat ccaggttcga
    12661 ttcctggtga cgggaccact cattcgcgtt ttcggtgttg taaacggttg gtcgcggctt
    12721 gctcggcagg cgctcgattc ggggaccgga ggcgtgcttc ccgatcgcac gagcccgatg
    12781 ccccgctact cccaggtgct gttcgacctc gacggcaccc tgatcgactc cgcccccgcc
    12841 atcctcgcga gctaccgcga cgccttcgcc agcacgggtc accagccggt gatcgcgatc
    12901 gacgcgggca tcgtcggccc gccgctgctc gagaccctgc gcatgctcgc cgggaccacc
    12961 gacatcgggg tgatcgacgc gctcgcagcc ggcttcaagg cgagctacga cagccgcggc
    13021 taccgccaga ccgcggccta cgccggcgtc gacgcgatgc tgcgccgcct cggcgcggcc
    13081 ggctgcacgc tctcgatcgc gaccaacaag cgcctgctgc cgacacgact gatcctcgag
    13141 cacctgggct ggagcgggta tttcgccgcg gtgtatgcgc tcgacctgtt cgagccacgc
    13201 ctgcccgaca aggcctcgat gatcgcgcgc ctgctgcagg atcgcggcat cgcccgtggc
    13261 gatgcggtct atgtgggcga ccgcagcgag gacggcgaat cggccgacgc caaccggctg
    13321 cccttcatcg ccgcgacctg gggttacggc agcctggagt cgaccgagat ggcgccgcac
    13381 tggcgcgcgg tggccagccc ggcggcgctc gtcgacgcga ttctgggcga cgcctgacgc
    13441 ttgcgggcat gctcgtgcgc gcctagttcc ggccggcgat caggcgctgc aggtactcga
    13501 cggggatggc gtagctgatg cccgacggat cggcgagcac cttttccttg gtcgacttca
    13561 cgaacaccat gttgagcacg ccgatcacct cgccactggc gggatcgtag agcgggctgc
    13621 cactgttgcc cggataggcg gtggcgtcga gctggtagac gcggaacgga tcggtggcga
    13681 gacgccggat gagcgccggg ttgagatcgc gagaattggc ctgaggaatc gcgatcggcg
    13741 tgatcgccga gacgatgccg cggtgcgtca ccggggtcat gccgagcacg gcaccgatcg
    13801 ggaagccggt gaaggcgacc tcctggccct cgaccacgcc gcccccgccg gcgagacgaa
    13861 gcgcaggcag cggcggcccg ccttcgagcc ggagcaaggc gagatcctgc gcgcgctccg
    13921 tggccaccac cttcaccggt cgcaccgctc cggtgaggtc gtcgcccgga agcacgatga
    13981 cgagcgcctc catcttcgcg ccgtcgatct cgtcaggcag gacatgggca ttggtggcga
    14041 ccagcatgcc gtcgccgacc gcaaagccgg taccacggaa ctggaaggcc gggctgcgtg
    14101 tacgctgata ggtaccgacg gcgacgaccg agggcttgac gcgcgccagc gtggacgcca
    14161 gctcggcacg tgcgatcggg gccagggcga gcgcggcggg aagggcgagc gcgcaggcca
    14221 gcagccgacg ccgcgcacgc gtctccgggc ggggattcat gactcgtgtt cctcttccag
    14281 ggcgaggcgg agcttgcgca acatcgggct gggggggacc acgcggctgt cgtagggatg
    14341 gcgatcctga ccgacgtgac gcaggatgcg ctccacgtag gcacgcgtct cgcgatacgg
    14401 cggcaccccc cgatagcgat ccaccgcccc ctctccggcg ttgtaggccg ctgcggcgag
    14461 cgcgatgtcg ccctcgaaat aggccagcag ccagcgcagg taggcgaggc cgccacggat
    14521 gttctgctcg ggatcgtagg gcttgccgac gttgaagcgc tcggccgtgg cgggaatgag
    14581 ctgcatgacg cccatcgcgt tcttgggcga gagcgcgtcg gggttcaggg ccgactcggt
    14641 cagcccgatc gcgagggcga actcggggcg cacgccgtac ttgggggcca gctcggcgag
    14701 catgcgcgca accgcctgac gcaccgccgg caggcgcgcg atcagcgcct cgaccggcca
    14761 gcgctgcacg atctgggtcg aaggcgtgtg caggcagtcc ggtacgtcac cggtgtattc
    14821 gccggtgtac cgcaacatgc gctgcgcatg ttcatcgcct ttcgcggccg ccatcgcgaa
    14881 gagcgtgccg gcgtaggcgt cgttgcgctc gacgccacgg ccgttggcgt acatccagcc
    14941 aagcgcgtac atgccctcgc tgtcgccgag gcgcgcggac tcgcagtaga gcaaggccgc
    15001 atacgactgg tcgcgcggca cgccctcgcc gtgttcgtag gccagcgcct cggtggcgag
    15061 atagcgcgcc tgctcggcga gcgcacgctc gaacgcgccc tcaccggccg cggcgcaaac
    15121 ggtcgaggcc agcgcgaccg ccaaccccag cagccgtgga aggcgccgga ggcgtggccg
    15181 cgcaatcctg ccgaccggga ccgggcaact tgtcttgtcc atgactcttt atcgcacacg
    15241 ccacgctgga tcgccaaccc tgggatcggg ggatcagagc gacgcttgca gcgcacgcgc
    15301 ctcctgatgc atcggggtcc cctcctgcag gcgcggcatc agcgcatcga gctggctgcg
    15361 cgcctcgtcc ttgcgtccgg ccttggcgag cgccttcacg aggttcagct gcagcggcag
    15421 caggtcggga ccgatcgaca ccgcgcgccg caggttggcc aggccggcat cgaccgcccc
    15481 gctggcgacc tggatggtgc ccacggtatc gatgatcgcc gggttgtccg gggcgaggcg
    15541 cagagcctgc tcggcatact ccagcgcctt cgggtccttc gcttcgttgg cggcccaggc
    15601 cagattgttc aggatgatcg ggttctgcgg cgccatctcg gccatgcgcg caaagatcct
    15661 ggccgcatcc gcataccgtt tctcgctcag ggccagttcg ccaagatggc cacgcagcac
    15721 gagatccgtc ggattcgcct tcagccaccc ctcggacatg cgattcgcat cggccctgcg
    15781 gtctgcgcgg agaagggccg aatgcaggcg taccgcagcc gtgcctccac ccttgcgctc
    15841 gagcgccttg cgataagcct ccgcggcatc gggccactgg ctgcccaccg catgcacgtc
    15901 gccctcgagc aggtagcctg caggaagctc gggctgccgc gactgggcct cgcgggcaag
    15961 acgcaacgca ccctgcgggt ccttgcgctg cagcgccagg gcgatcaggc gctggcggac
    16021 gtccatggag tcgggggcga tccccaaggc cttgcgcaag gcctgctccg ccgcctgtcc
    16081 gtccatcagt gcacgatgca tgtccgccag cgcaacgagc ggaagcggcg actcgggcaa
    16141 tagcccgacc agccgattga gcgcggaaat cgcttgctgg ctgtcccccg cggcgaactg
    16201 cgcgcgcgcg agcgcctcga tcgcgcgcgg atcgttcgga tgcgcggtcg cgagcttctg
    16261 cgccacccgc agtgcttccg caggctgccc ttcggagaga tggtgctgga tggttgccag
    16321 accgacgacc ggggccccgg gcgagacggc ctcggcacgc tccagcgttc cgagcacctc
    16381 gatcggcgcg gcgccggtga gacgctgcat ctcggcgaag gtcagcaagg cctcgacact
    16441 cctggggtcg cgatcgacca ccgccttgat gcgcccgatg gccatctcgg gacggcgctc
    16501 ggccagatcg agccgcgcca ggctgaccgc ggcggagaga taggtgggct gcttcgcgag
    16561 tgccttctcg aacgctgcac gcgcagcagg aatgttgcgc gtggccagca tcagcccgcc
    16621 acgaagattg tgcacgagcg cattgtccgg ctgcttgcgc tcgagttcgg cctgtgcggc
    16681 gagcgccttt ttcgcgtcgc ccctgcgcag atgcccgacg acgagggcta gatcggcctg
    16741 gatgccgccc tcatccatcc gggaggcttc ctcgagttcg gtgaagccgg catccgcgtc
    16801 gccgacggcc atgcgcgcga ccccaaggcg cgttcgcgca cgcacgtccg ccggtgctgc
    16861 cttcgccgcc cgttcgaaat agtcctcggc acgctcgaaa tccccattgg cgatgtacac
    16921 ctgccctgcc aggccgagca ggcgggcact cggctgcgga acctggagcg cgggctggac
    16981 gagctcgagc gcacgcttcg actcgccggt ggcgaggtgc gaggccacca acgcctggcg
    17041 agcgacgaga tttcccggcg cggcctggag aacccggtcg agatgggcac gcgcgatctc
    17101 gtgatcgttc agccgcaaat gcacgacgcc tgcgagcagt tcggcgacga gcagcccggg
    17161 cgactccttg agcacctgca tgagcgcgtc gcgggcttcc acgaggcgat tctcgcggta
    17221 atcccgcagc gctaggaggt aacgcgtgaa ggggtgcctg cccaccgccg acttcatcgc
    17281 gtcgagggcg gcatcggcct cggcgatggc gttctggcga agcagaaggg tgacgagcgc
    17341 gtaatggttg gccaccgtcc ccggttgcag acgcacggct tcgcgcaagg cggcgagccc
    17401 ctgcgacaga tcgccgcggg caacgagcag gtcgccgagc aacgcatgcg catcggcgac
    17461 ctgcggcgcg cgtgcgatga cctcccgtac ggcctcttcc gctgccttca gatcaccctt
    17521 gatcgccgcg gtgcgtgcga atccgatccg cgccccgtgc tcgtcggcgt tcgccccgag
    17581 cgcctggaga taggccgcct gcgccttgtc gatctcccgc cgcgccagat gcgcatcgcc
    17641 cagcgcagcc tggacgatgg cgatcgtcgc gggcgcggtc acccgaggat cgccgaactc
    17701 cgcgagaagc cggtcgaact cgcccgcccg caccagcgcc ggcgcaagtt cggcggcgac
    17761 cgcgtcgcgc gggtagccga gctcgagcgc ccgctcgagc tccttgaccg cgccagcgac
    17821 gttgccctga cgcaggttga cccgcccgag gagaaaacgc gcctcggcga gattggcgtt
    17881 ttcctgcagg gcattcttga gctggatgcc ggccgcgtcg aagtcctgct tctccagaaa
    17941 ggacttcgcc gaggcgagca tctgctcggg cgtctcgcca caaccgacaa gaacgagcgc
    18001 cgagaacgcg gcgacggcga tgccccgaat gggccgggac cagcgcgggt ggtgacggac
    18061 ggtgcggttt tcgctcacga agcattctcc ttcttgagtc cgaagcggct caatagatcg
    18121 tacagggagg ggcggctgac accgaggatc tcggcggcgc gggcgatgtt gccgttggtg
    18181 cgggcgatga ccttgagcac ggcggcacgc tcggcatcct cccggacctg gcgcaggttg
    18241 aagatcgaaa ggtcttcgtc ccccgccgcg aggccgaggt cttcggctgc gatacattcg
    18301 ccatcggcca tgatcaccgc acgcttgatg cagttttcca gctcgcgcac gttgcccggc
    18361 cagcggtgcg cctcgatcgc cgagagggcg tcgtccgtga agatgggcgt gcgccggccg
    18421 ttctccttgg cgaagcgctg tgcgaaggca tgcgcgagat aggtcgcgtc gccgtcgcgc
    18481 tcgcgcaggg gcgggatttc gatgacgatc tcggcaagcc gatagtacag gtcctcgcgg
    18541 aagaccccgg cctggatctg cgccttgagg ttgcgatggg tggcacacac gatgcggacg
    18601 tcgaccggga tctcctcgcg cccgccgatg cgctcgatcg agcgctcctg caggaaacgc
    18661 agcagcttgg cctgcagcgc gagcggcagg tcgccgatct cgtcgaggaa gagggtaccc
    18721 ccgttggccg tctcgatctt gccgagggtc tgtttcactg cgccggtgaa ggcgcccttc
    18781 tcatagccga acagctcgct ctcgagcaag gcatcgggaa tggcggcgca gttgatcgcc
    18841 acgaagcgct cgcgggcgcg ggcggagcgc atgtgcaggc cgcgcgcgag gatctccttg
    18901 ccggtaccgc tctcgccgag cagcgcgacc gtggcggagg ccgaggcgag cttctcgatc
    18961 gtgcggcaga ccttgagcat cccgggatcg cgggtgagca gtccggagag cgggctgccc
    19021 tcgagcgcgg cgaggcgggc gttctccctc tgcaggtcgt gcatgcggaa ggcgcgctcg
    19081 atggtgagat tgagcagctc gggctcgaag ggcttggcga agaagtcgta ggcgcccagg
    19141 cccaccgcct tgatcgcgtt ctcgcggtca tgctggcccg tgagcacgat gaccttggta
    19201 tcgggcgcga gcgtgaggat ctcgcgcagg agcttgaagc cctcgctgac gtcgtcgggc
    19261 gccggcggca ggccgaggtc catggtcacc accgcaggtt cgtgtttgcg cagctgcgcg
    19321 atcgcgctct cgcggtcgct cgcgaccacg gtctcgctgg cgtcgaacgc ccatcgcatc
    19381 tgcttttgca gcgcgaggtc gtcctcgacg atcaggagag tacgctgctt gtcttgcgtc
    19441 gaattcacgt tctatatgcc catcgcggta gattgcccgt cctcgcgccg gatgcggggg
    19501 agttcgatac tgacgcgggt gccgcgcccg acttcgctgt cgtagtgcag cgcgccaccg
    19561 atctcctcga ggtactgccg ggtctcgtag accccgatcc ccatcccgct cgacttggtg
    19621 gtctggaagg gacgggacag ccgttcgcgg acgaattcag gactcatccc acagccgtcg
    19681 tcctcgacga ccacgttcgc ccgcccgtcc ttcccggcag cgagactgag aaccacctta
    19741 ccatcctgtg ccgtggcatc cagtgcattt tgcacgacat gaccgatgat tctctccagc
    19801 cgctgcgggt gcgcactgac ctccaccggt cccgcggctg cgcacacgag ctcgatcgcg
    19861 ggcgactggc cacgcttgga ctcgcacacc gcgcgcagca gcgcggggag atccaccgcg
    19921 cgcggcgggt cgatcgagcg cttctcctgc aactgcgtca tcagcccgcg catgcgcgcc
    19981 tcgacgttcg ccaccgtatc cagcatgtct tgctggaact cggggttgtc gcggtggcgc
    20041 tgggcgttct tgagcatcag cgacaactgg gcgacgaggt tcttcaggtc atggaccacg
    20101 aaggccgaca tgcggttgaa ggagtcgaac ttgcgtgcct ccagcaaggc ctcggccgcg
    20161 agcatacggt ccagatagcc tgcggcctga cgctgcgccg tcttgaggag atcgaggact
    20221 tcccagtcga tgtcgaaggg ggtccgcggc gaacccagta cgacgaagcc gatcaggcgc
    20281 tcggcggtct gcagcgggat caccagccat gcctcgtcga gccgtgacag ccagtccggc
    20341 agcaccagac cctggtagcg tccgggctgc acgcgatact cctcgaggtt gatcacccag
    20401 ccgctgcggg caaggaaccc gcacagcggc gaatcgagcg cctcgaccac gtcgatcggg
    20461 ggctggttga agcgcgaatg cggccggcac ttgccgtagg catcggccag ccagagcgac
    20521 cctccagggc tctcgacaag atccgacagc gccttgatga ccgattgacc cagatcgaga
    20581 ctgcggtcga cggtcgacag ggcctgggtg aagcgcaacc actcgctccg gtaatcgtag
    20641 cggtacggaa agaggtgctt gctgatgaag acccgcaggc gcgcgcgcac ggcgcccgaa
    20701 aacagcagcg cgccgaggac caccaggccg gcgaagagca atgccatctg cagtgcgcgc
    20761 ccccaatcgc cgccgaagaa gcgcacgtaa taggccgcag cggcgatcac gagcaaatag
    20821 aggccggcgc cggcgagcgc ggtcgaatgg aagacgatct cgcgcgatac ggacatccgc
    20881 agggtccatg agggactgcg cgtccccgcg attgcgatca gcgggatgag cagggcgtgc
    20941 gcgatgccgc gcgctgccca gacatccgca tcgatgcgcg agaacaggag ggcatcggcg
    21001 aagaggtaca gttcgaagat gtagcccgcc gccagcgcga ggcaaagcgg cttcaaaccc
    21061 cagcgcgaga cggcgggcat gccccggtag agctgttcca caagcatcag gccgacgatc
    21121 gcgtgcatca gccaggcacc gatcgccggc gcgatcggca accctccggt gacgcccgcc
    21181 gactcgagcg cgagcgcgag cacctgcgcc accaccaccg cgagcgccac atacgccggc
    21241 cagcgcatcc accgccccca caacgggcgg gacagcacga tcaggaacac gtaccaggcc
    21301 gcgcccctga gcagatcgag cgcgacggcg agggcaccgg aaaacggcca agcgcccctg
    21361 gcctcgagcg ccgaaagacc cgcccacaga ccggaaatcg ccacggccgc gaacaaggcc
    21421 ccacccggca gcgcgccccg ccacgcgagg aagatgtaca gcgcgaagat gaggaaggct
    21481 acggccgcaa ccccataact ccagatcgca acctcaagca cgcccctctc ccctgtcacg
    21541 cattcgtgcg aactgcgccg tacccccgtc caagcgagcc cgcagtctca cgcggccgct
    21601 cagtgcgcgc cttcaccggt caggacgacc ctcaccgtct cgaagaggac aagggtatcc
    21661 aggatcagcg agtggttctt cacgtaatac agatcgtact ggagcttctc cgcggcatcg
    21721 tcgaccgaag ccccgtactg gtaacgaacc tgcgcccacc cggtgacgcc gggcttgacg
    21781 cagtgccgca ccgcatagaa cggaatctgc tgggtaagct ggtccacaaa ataggggcgc
    21841 tccgggcgcg ggccgaccag actcatgtcg cccttgagca cgttgaacag ctgcggaagc
    21901 tcatcgatac gcagcttgcg gatgaaacgc ccgacgcgtg tcactcgatc gtcgttcgaa
    21961 gtcgcccacc gcggcttgcc gtccttctcg gcgtcacgcc gcatgctgcg gaacttgatg
    22021 acacggaacg gcttcccacc gcgtcccaca cgctcctgcg agtaaaagat cggcgcgcca
    22081 tcctccagca ggatcaacag cgcagtgagc accatgatcg gcaaggccac gagcaggagc
    22141 gcggtcccga cgacgaggtc gaaacaacgc ttgacgaagg ttcgcgtcca gccttggcgg
    22201 aagccgtcac catagatcag ccagctcgcc cgcagcgaat ccagcctcac ttgcccctgc
    22261 acgcgctcga agaaggtcga cagatcgagg atgcgcgttc cgagcaactt gcagtccagc
    22321 agttcgcgca aggagagcac tcccccccga cgttcgcgca ccgcgacgat cacctcgtcc
    22381 acacgcaggt tgcgcaccac ctccagcaag ctgcccggtg gcaacacggc gtgctgctcc
    22441 acctcgaccc gttcgtttcc ccccaccgga agaaagccga ccacttccac gctgcgctgc
    22501 agcggacgga ccaggtcctg ttgcaccatg cgcgcgtcac ggccggtacc cacgatcagc
    22561 acgcgcggca cgaaaagggc agaggcctgg cgctgattga ccagcccgcg gaccagcaac
    22621 agcgaacaga gcaacagcaa tacgctgagc tgcagggagt gcggggcaat ttcggaccac
    22681 ggcaacagcc cgaacacgag gtaggccact ggtacgctca cggtcagcga cagggcgacg
    22741 cgcgcgaagg tctgccgatg ggaacgctga tacaccggtc gatacaaacc catcgccgca
    22801 ttgagggcga tcatgcttgc agcgaaggta agcgccgatg gaatgcagat tgcccagtcg
    22861 accgcgcttg tcggcgtcac gagtaccgca accgctgcaa gcaccaccgc aaaaagcgtc
    22921 aaggcgtcga acagcacctg ctgcagggtg tgcgtgggaa ggtagtggct gaacaccttc
    22981 agcatcgagg ggcctctgca tgtgttggga agggtcggaa ccctgggcgc cgggagtgct
    23041 tatcgcggag ggatcgcccg gctcctgatc gtgcctaagc ttagacaatc accggcagga
    23101 cacctgtgac tcacttcacg gcgagggcgg gtggagcaaa ccgcctcgag cacaccgagc
    23161 aaaatcgcac gaaacgcccg cctggccttg cggcaaatgc ggcagcgcag catccaaggt
    23221 tgattttagc atgcgaagct tgtagcgtcg gtccgttacc ggagcaccgt ttcacacaag
    23281 gagaagtaca tgactcaagc tcaacgcgat ttcagcgaag tctacggcct cagtttcgag
    23341 gaccttcaca ttggccgtac cgcctcggtc tcacgtacgg tgagcgaggc cgacatcctg
    23401 atgttcgccg gcgtatcggg cgacaccaac ccggtccatc tggacgagga gttcgctgcg
    23461 agtacgatgt tcggcggccg gatcgcccac ggaatgctct ccgccggcct catctcggcc
    23521 gtcttcggca cccgcctgcc cggccccggc tgcatctacc tgtcccagag cctgaaattc
    23581 aaggcgcccg tcaagatcgg tgacaccgtt gtcgcgcgtg tcaccgtcaa ggaattgaag
    23641 gcagagaagc gccgcgcggt tttctcgacc gtctgcacag ttggagacaa ggtggtcatg
    23701 gatggcgagg ctgaaatcct cgtaccggcg cgcacctgat cgagcatgcc ctcgcctcgc
    23761 gcggctccct gcagaggccg tgcgaggcac ctgcagaaat ctcgccaccc agctctaagc
    23821 ccccggcgaa cccaccttcc gccgtctgct tatcccattg aagatcgtgt ccatcaagtt
    23881 cgatggtgga cacgatggat gtggttatcc cgcgccggag tcggcgcacg cacagcgaag
    23941 ccttcaagca gtctgtggtc tcggcctgtc gtgagcccgg cgtctcggtg gccggcctgg
    24001 ctctggccaa cggcctgaac gccaaccggg tccaccgctg gatgcgcgag cgcggcatcg
    24061 agccaccgag tcctcgcaag ccgctctgcg ctgaacgccc gccattggct gagccgggct
    24121 ttgtagccgt gcaggtggct cccgccgtcg aaacgttggc gatccgcctc gaagcgcagc
    24181 gtggcaacgc ccgcattgag ctcgaatggc cgctccaggc cgctgcccgc cgacctcgat
    24241 caactggatg ccgatgcctt gcgccacctg ctcatcggtc aggagcgcga gctcgtgtgg
    24301 cgccagacca agatcgagcg gctcacgcat gagctggcgc tgcaca
//
LOCUS       DAOQBS010000037        23979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1357_length_23979_cov_14.527964, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000037 DAOQBS010000000
VERSION     DAOQBS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 23979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23979
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1357_length_23979_cov_14.527964"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(444..1376)
                     /locus_tag="PKN25_06195"
     CDS             complement(444..1376)
                     /locus_tag="PKN25_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNV90525.1"
                     /translation="MNDQNPTLAASDAPVPVAHPAVRPDPRSDGEFLLALGRRVRETR
                     DRRGLTRKQLARDAGVSERHLAHLEAGEGNVSIVLLRHIASALGLSLPEMLNLGAEES
                     VEQRLVRRILEQLPRHRLEDVVFRLMRDYGQEEAARRNRIALIGLRGAGKTTLGRRLA
                     AELGFRFVELDAEIERETGMPMGEIFALYGQSGYRRIEQRCLRRALDSGERTVLATGG
                     GIVSQTETYDLLLSRCLTIWLRTSPEEHMQRVSAQGDLRPMAGSAEAMEDLRRILAAR
                     EPQYLRADHVVDTSGQGEDESFRALRAAVEDRGR"
     gene            1751..3337
                     /locus_tag="PKN25_06200"
     CDS             1751..3337
                     /locus_tag="PKN25_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HNV90526.1"
                     /translation="MHTLSTADHGCTPPRIVIPDPYNAADDLLGRNLVAGRGSKVAYI
                     DDAGSYTYDQLAARANRCGNALLGTLGLARGDRVLMCVLDCIDFPAMFLGAIKAGVVP
                     IAVNTLLTERDYEYMLSDSGARVAVVSQALLANFAALPGRVPTLERLVVAGGDDDDGL
                     EALLAAQSEQLDAAPTRATDPCFWLYSSGSTGAPKGTVHIHSNLIHTAELYGQEVLGI
                     REQDVVFSAAKLFFAYGLGNGLTFPLSVGATAILMAERPTPDAVFKRLREHQPDIFYG
                     VPTLYAGMLAHTGCPTAGEMKRLRACTSAGEALPEGVGRRWKERIGVDILDGIGSTEM
                     LHIFLSNRAGDVQYGTSGTPVPGYRVRLVDEGGDEITAAEEPGELQISGPSSAVQYWN
                     QPEKSRATFLEGWTRSGDKYYRNRDGYYVYSGRSDDMLKVSGIYVSPIEVESALIAHE
                     AVLEAAVVGIEDEERLIKPKAFVVLKPGYGPGEAMRQELQAHVKGMLAPYKYPRWIEF
                     VEDLPKTATGKIQRFKLRGI"
     gene            3399..4586
                     /locus_tag="PKN25_06205"
     CDS             3399..4586
                     /locus_tag="PKN25_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV90527.1"
                     /translation="MVGKDRKLRLAVSALGLGLGMLLAQGAAEAADKVKVGLMLPYTG
                     TYASLGNAITNGFKQYVAEQGGKLGGREVEYFVVDDESDPAKATENANKLVKRDNVDV
                     LVGTVHSGVALAMAKVARDSKTLMIIPNAGADELTGPLCAPNVFRTSFSAWQPANAMG
                     KVVAERGHKNVVTLAWKYSFGEQSVAGFKESFEQAGGKVVKELYLPFPNVEFQPFLTE
                     IANLRPDAVFVFFSGAGAAKFVKDYEAAGLKAGLPLYAPGFLTDGTLEAMGGAGEGVL
                     TTLHYADGLDNAREKSFRSGYVAAYKAQPDVFAVQGYDSAQLLAAGLSGAPAGAFDKE
                     AVMKAMSAATIDSPRGSFTLSKANNPVQDIYLRKVEGGQNKVIGVAAPKLADPARGCK
                     LMN"
     gene            4700..5566
                     /locus_tag="PKN25_06210"
     CDS             4700..5566
                     /locus_tag="PKN25_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV90528.1"
                     /translation="MDFTSFLIQILNALQYGLLLFLVASGLTLVFGIMGIINLAHGSF
                     YMIGAYLAFALTSLTGNLMLAIVLGIPLAFGFGALLEKLLFVHLYKRDHLQQVLLTYG
                     LILIFEELRSLTVGDDVHGVAIPDLFSASIALSDNLSYPVYRLVISVVCIALAVGLWW
                     LMQRTRLGMMIRAGSFNREMVQSLGVNIDLLYRTVFALGVALAALAGMLAAPISSVYP
                     GMGGNVLIISFVVVVIGGIGSVWGALVAALLVGFADTFGTILLPELAGLAVYLLMAVV
                     LLWRPEGIFRKG"
     gene            5571..6539
                     /locus_tag="PKN25_06215"
     CDS             5571..6539
                     /locus_tag="PKN25_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV90529.1"
                     /translation="MNPRVVLSFKLAVLAGLVLFPAIGSSFYVELVAKILVMSIFAMS
                     LALLVGFTGLVSLGHAAYFGIAAYTVALIAPEYEAANLWFVLAASVLAAGAAAFFIGL
                     FVLRTKGIYFIMVTLAFAQMAYYIFHDTALGRSSDGIYLNVKPDASLFGWMPFNLDEP
                     LHLYYFILAAMVAVFVLLAFVLRSPFGRVLAGIHSNEHRMKSLGYATQRYKLAAFTLA
                     GALAGVAGFLYAVLFGFVTPDYLSWHQSGNVLLMVILGGLGSLAGAVAGAFAFIGLQE
                     MFSDWTKHWQLLMGLVIVAAVLFMPSGLAGLPRRIFRTVNWGEDDE"
     gene            6532..7287
                     /locus_tag="PKN25_06220"
     CDS             6532..7287
                     /locus_tag="PKN25_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90530.1"
                     /translation="MSEAMLVADRLTRHFGGLTANLEVCITLERGKLHALLGPNGAGK
                     STCINMLSGDLPPSAGRILLEGQDVTGLDSAARSHRGIGRSYQRTNIFPQFTVLENCR
                     LAAQSRRPRPWRIFTDAMKTRDTVERAQAAIVEAGLGGRELRKAGLLSHGEQRQLEIA
                     MVLATDARVLLLDEPLAGMGSEESAQMVELLRKLKQNRAILLVEHDMDAVFAVADTIT
                     VMVEGSVLESGPPEQIRNSEDVQAAYLGEDEHG"
     gene            7280..7993
                     /locus_tag="PKN25_06225"
     CDS             7280..7993
                     /locus_tag="PKN25_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90531.1"
                     /translation="MAEMLLEARDLHTYYGSSHILHGVSFSIRKGEALGLMGRNGMGK
                     TTLIRSMLGLVRPRHGTVRVNGDDMTRAKPHRIALAGVAYVPEGRGIFPNLSVRENLV
                     MAARAGVDGRREWTFERVMETFPRLGERIDNGGGQLSGGEQQMLTIGRALMTNPDLLI
                     LDEATEGLAPLMVKEIWKVIRTIRDTGIATVVVDKNHSAVTALTNRSVILVKGEVVFD
                     GPSEQVRQDPALIRKHLGV"
     gene            complement(8084..8479)
                     /locus_tag="PKN25_06230"
     CDS             complement(8084..8479)
                     /locus_tag="PKN25_06230"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNV90532.1"
                     /translation="MQILQPAGWKRPKGYANGVAASGRMLFVAGQVGWDADEVFRSED
                     LVEQIRQALRNVLAILDAGGARSTDIVRMTWFLGSAAEYNARLEEIGTVYRDLIGHHY
                     PAMSAVQVAGFVEAGAKVEIEVTAVLPDD"
     gene            8771..10954
                     /locus_tag="PKN25_06235"
     CDS             8771..10954
                     /locus_tag="PKN25_06235"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HNV90533.1"
                     /translation="MTERVSVQRLQVAANLKRFIEEEALPGSGVDAAAFWSGFDALVH
                     DLAPKNAALLAERDRIQAEIDAWHRAHPGPIADMAAYKAFLSSIGYLLPVPAGAKATT
                     TNVDDELAVQAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAIPQKDGAELTKGY
                     NPVRGAKVIAFGRQVLDQAAPLAGASHADAAGYAVEAGRLVVKLQSGASTGLQQAEKF
                     VGFQGEAAAPRAVLLKNNGLHIEIQIDRSGAIGKSDAAGVNDLLMEAALSTIMDCEDS
                     VAAVDADDKVVVYRNWLGLMDGTLEDTFDKGGKPMTRRLNADREYTGADGKPVKLHGR
                     ALLFVRNVGHLMTNPAILWGEGKEIPEGIMDAVVTTLIAKRDLERRGNSRKGSIYIVK
                     PKMHGPAEIGFADELFTRVEQLLGLPANTVKLGIMDEERRTSVNLRACIAAAPARVAF
                     INTGFLDRTGDEMHTSMEAGAMMRKGDIKNSKWIAAYERRNVLIGLAAGLRGRAQIGK
                     GMWAMPDLMAAMLEQKIGHPKAGANTAWVPSPTAATLHALHYHQVSVAAVQQDIEKLD
                     LDKEAEALLDDLLTIPVVAKAEWSETERQQELDNNCQGILGYVVRWVEQGVGCSKVPD
                     INDIGLMEDRATLRISSQHIANWLRHGVVPAEQVEATLERMAAVVDGQNAGDPLYRPM
                     APNFDDSVAYQAARALIFEGCAQPSGYTEPLLHRYRQQFKAKVGA"
     gene            complement(11144..11953)
                     /locus_tag="PKN25_06240"
     CDS             complement(11144..11953)
                     /locus_tag="PKN25_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNV90534.1"
                     /translation="MARFTPGSLTAKAALIGALLLGGCGLLPEQVDETAGWNAQKLYS
                     EAKASMSEGGYDRAVTLFEKLEARYPYGRFAQQAQLEVAYAYYKQGEQALALAAADRF
                     IKLHPNHPNVDYAYYLKGLVNFNEDLGLLAGLSRQDLSERDPKGAREAFDSFRELVER
                     FPESRYADDSRARMQYLINSLASHEVHVSRYYYNRGAYVAAINRAQTAVNNFPQAPAI
                     EEALFLMVMSYDKLGMAQLRDDADRVMRKNFPDSVYFRGGPEDTRPWWQLW"
     gene            11967..12950
                     /gene="rluD"
                     /locus_tag="PKN25_06245"
     CDS             11967..12950
                     /gene="rluD"
                     /locus_tag="PKN25_06245"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HNV90535.1"
                     /translation="MNEHADYIPAALPADLPADDADAWEALTIPVELGGLRLDQALAR
                     LLPQHSRSRLQGWLKEGWIRVDGASPDARRKVCGGECVEIDAPPPPEVTAALPEDIPL
                     AVVYEDEDLLVIDKPVGLVVHPGSGNWSGTLLNALLHRNPALAGVPRAGIVHRLDKDT
                     SGLMVVAKTLAAQTELVRQLQARTVGRHYRALVQGALTAGGTVDAPIGRHPTQRTRMA
                     VVGNGRPAVTHYRVTERFPRATLVECRLETGRTHQIRVHMAHIGHALVGDPLYGARRC
                     GDVRLDAFPRQALHAFRLGLTHPRSGEAMRWEVPLAADFAALLDELHGAPR"
     gene            12947..13729
                     /gene="pgeF"
                     /locus_tag="PKN25_06250"
     CDS             12947..13729
                     /gene="pgeF"
                     /locus_tag="PKN25_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNV90536.1"
                     /translation="MSLPPGYRFFAPGLLCPQWSLPPGVRAVVSTREGGASLPPWASF
                     NLGSHVGDDARAVAANRARLGAAIGAEPVWLEQVHGCAVADADRAAGVPEADAAVARM
                     RGRACAVMIADCLPVLFCDDDATVVAAAHAGWRGLAAGVLERTVERMGVTPGRVRAWL
                     GPAIGPTAFEVGDEVRAAFVGADPGATAAFVAGGVPGKWLADLCALARRRLTLAGVER
                     IDGGSVCTVADPAHFYSYRRDGTTGRFAALIWLEEGRPGAIA"
     gene            complement(13779..14000)
                     /locus_tag="PKN25_06255"
     CDS             complement(13779..14000)
                     /locus_tag="PKN25_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90537.1"
                     /translation="MYLIPIAWLYVVVLVAAAEDSIVAAVLTFVFWGLAPLALFLWLF
                     GTPARRRRRLQQEREALERNDRAETDADQ"
     gene            14107..15852
                     /gene="phaC"
                     /locus_tag="PKN25_06260"
     CDS             14107..15852
                     /gene="phaC"
                     /locus_tag="PKN25_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNV90538.1"
                     /translation="MSDTTGKQVPPGMQAILAAGQAMAQGFFDALARQHVAMQDSSGV
                     AAQKLPMPETEVMASLQKVFAEKHAELWSSMLARTPGAAGPAVVQPEPGDKRFAAPEW
                     SESPVFDYMRQAYLLNAGFLRQMADAMPIADGRAKARMQFLTRQYIDALSPSNFAATN
                     PEFIKTAVETKGESIARGIQNLLGDLEKGRISMTDDAAFEIGRNLALTPGSVVYENEL
                     MQLIQYAPLTEKVAQTPLLIVPPCINKFYIMDLQPENSLVRFVVEQGFTVFLVSWKNP
                     RPDTGGHYTWDDYLDKGPLAALEVVRSITRVKKPNVLGFCVGGTILTSALAVARARGE
                     EPVASLTLMTTLLDFSDAGELGCLVDEASVTAREAAIGKGGVLKGQELANVFSFLRAN
                     DLVWQYVVGNYLKGNKPVAFDLLYWNSDSTNLPGPFLTWYLRNMYLENNLRVPGKLRM
                     LGHKVDLGKVDVPAYLMAAREDHIVPWKSAYLARNVLGGDTTFVLGASGHIAGAINPA
                     SKNRRSYWTADVRPADPDEWFESAAEHKGSWWLHWAEWLRGHGGKEVAARGRLGSTKY
                     EPIEPAPGRYVKERS"
     gene            15968..16708
                     /gene="phbB"
                     /locus_tag="PKN25_06265"
     CDS             15968..16708
                     /gene="phbB"
                     /locus_tag="PKN25_06265"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HNV90539.1"
                     /translation="MARVALVTGGMGGLGEAICIKLAALGYKVVTTHSPGNTKAQEWL
                     QTMNNMGYGFKAVPCDVADFDSCKACVEQVVKEVGPVDVLVNNAGITRDMTFKKMTKA
                     DWDAVISTNLDSAFNMTKQVMDGMVERKWGRVINVSSVNGQKGAFGQTNYAAAKAGMH
                     GFTKALALEVARNGVTVNTISPGYIGTKMVMAIPQEILDSKILPQIPVSRLGKPEEIA
                     GLVAYLSSDEAAFVTGANISINGGQHMF"
     gene            16947..17687
                     /locus_tag="PKN25_06270"
     CDS             16947..17687
                     /locus_tag="PKN25_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HNV90540.1"
                     /translation="MSKKIALVTGAMGGLGTAICQSLAKDGFKVVANCLPNFELKDAW
                     LSAQRDLGFDFAAAEGDVSNYESCAAMVAKIEADVGPIDVLVNNAGITRDKFFPKMEK
                     GQWDAVINTNLNSLFNVTHHVSAKMAERGWGRIINISSVNGVKGQAGQTNYSTAKAGV
                     LGFTKALAAELATKGVTVNAIAPGYIGTDMVMAIREDIRQGIIDTVPMKRLGRPDEIG
                     DLCAYLASDKAAYITGATININGGLHMC"
     gene            17818..18411
                     /gene="phaR"
                     /locus_tag="PKN25_06275"
     CDS             17818..18411
                     /gene="phaR"
                     /locus_tag="PKN25_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HNV90541.1"
                     /translation="MPEQQRLIKKYPNRRLYDTRTSSYITLADVKDLVLGHEHFQVVD
                     AKTGEDLTRSILLQIILEEEAGGAPMFTSDLLAHMIRFYGNAMQGMMGKYLESNIKAF
                     TEMQVKLQDQARAIYGENSPVGQDLWAQFLNFQGPALQSMMGAYVDQSKKMFEQMQTQ
                     LESQTRNMFTGFQFPGYVRPEDAESAAPAALKGDGKK"
     gene            18466..19797
                     /gene="rimO"
                     /locus_tag="PKN25_06280"
     CDS             18466..19797
                     /gene="rimO"
                     /locus_tag="PKN25_06280"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNV90542.1"
                     /translation="MTTFKTSDLPRVGFVSLGCPKATVDSEHILTRLRAEGYAISGSY
                     DDADLVVVNTCGFIDAAVEESLDAIGEALAENGKVIVTGCLGAKDDVILAAHPQVLAV
                     TGPHATEEVMHAVHRHLPKPHDPFTDLVPPQGIRLTPQHYAYLKISEGCNHRCSFCII
                     PSMRGDLVSRPIHEVMREAEALADAGVKELLVISQDTSAYGVDVKYRTGFWGGRPVKT
                     RLLDLAKALGELGIWIRLHYVYPYPSVDDLIPLMAEGKILPYLDVPFQHASPRILKAM
                     KRPANAENVLERVRKWRSICPELTIRSTFITGFPGETEEDFEMLLNFLEEAQLDRVGA
                     FAYSPVEGATANELPGAVPDEVREERRARLMDFQEDISTQRLEAKIDREMTVLVDEVD
                     EEGALARSPGDAPEIDGLVVIPEGDGLAPGDFVRVRITDCDVHDLYAERIE"
     gene            19828..20802
                     /gene="rssA"
                     /locus_tag="PKN25_06285"
     CDS             19828..20802
                     /gene="rssA"
                     /locus_tag="PKN25_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HNV90543.1"
                     /translation="MLSGAGSVGLACAEPSGGGPVIGLALGSGAARGWAHLGVLLELA
                     REGVVPQIITGCSIGAFVGAAAASGDLDKLVRWAETLKWQDVVSLLDVSLRGGLIKGQ
                     KLIDFFERNFVDRDFTELDQRFACVATELSTGREIWLHEGSVSAAVRASIALPGLMTP
                     VLHQGRMLVDGGLVNPVPVSLCRAMGADVVIAVDLGSDMVGRAFRRSAVEPAPVEETE
                     AGWTERLLARFGFAAEAESGMPSRPLAAADSLPSLVSVISSSINIMQVRIARSRLAGE
                     PADLLVSPRVSQLGLMDYHRADEAIAEGVAAVARVRPLLRELLGGANE"
     gene            20810..22219
                     /locus_tag="PKN25_06290"
     CDS             20810..22219
                     /locus_tag="PKN25_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNV90544.1"
                     /translation="MSDELNARLRAIVGAANVLDGETDMAPFLSDWRGRYHGRARAVV
                     RPRDTAEVAAVVAACAQAGVAMVPQGGNTGLCGGATPLADGAAVVISLARLDRVRALD
                     PDNDTLTVEAGCTLAAVQEAAQAAGRLFPLSLASEGSCLIGGNLSTNAGGVQVLRYGN
                     TRDLVLGLEAVLPDGRVWDGLRALRKDNTGYDLKQLFIGAEGTLGIVTAAVLKLFPAI
                     RTRATAWVAVADPRAAVRLLGLLRAACGDRVSAFEIVGRTALGLVLRHIPGARDPLAG
                     APAWTVLVELSDPAVDAPLEAQLEAVLGEAVAQGLASDAAVAASVAQARALWALREDI
                     SEAQRIEGVSIKHDVSVPVSRIPEFLERAGAALAARWPDIRVVAFGHIGDGNLHYNLS
                     KAVADDNATFIARTAEVNGIVHDLVCELGGSISAEHGLGQLKREEVLRYKPALEMELM
                     RRVKQAFDPAGLMNPGKVL"
     gene            22283..22358
                     /locus_tag="PKN25_06295"
     tRNA            22283..22358
                     /locus_tag="PKN25_06295"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22316..22318,aa:Ala,seq:ggc)
     gene            22373..22448
                     /locus_tag="PKN25_06300"
     tRNA            22373..22448
                     /locus_tag="PKN25_06300"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22407..22409,aa:Glu,seq:ttc)
     gene            22509..22585
                     /locus_tag="PKN25_06305"
     tRNA            22509..22585
                     /locus_tag="PKN25_06305"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22543..22545,aa:Asp,seq:gtc)
     gene            complement(22699..>23979)
                     /locus_tag="PKN25_06310"
     CDS             complement(22699..>23979)
                     /locus_tag="PKN25_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV90545.1"
                     /translation="EAHVQAFGESAEPVCWMMIGYASGYTSAFTGRFILFRETECAAT
                     GHEHCRIVGRPAEDWPDADTLLPYFQADSIVGRLLELREEIEAMRRTISCPRRTPDLI
                     GSSAAFLHAYDLIGRAASTNVSVLLLGETGVGKERFARTLHEMSARKDEAFVAVNCAA
                     LPDELIESELFGVERGAFTGAHTSRAGKFERAHGGTLFLDEVGELPLPAQAKLLRALQ
                     EGEIERLGDERTRKVNVRLVAATNVDLQKAVNAGTFRRDLYYRLNVYPVTIPPLRERT
                     GDIPALVEAMVRRFETLHGKRIAGLSDKAMHALKLHAWPGNVRELENVLERGVILAPQ
                     NGQIEVEHLFIGTLDEHAREHSLSAQGGLETARRAQQDLAAAVLDSGMSLDAFEQRLL
                     QHAVERANGNLSAAARLLGMTRPQLNYRIKKQGA"
BASE COUNT         3805 a         8006 c         8258 g         3910 t
ORIGIN      
        1 tcgggctcat aacccgaagg tcgcaggttc aaatcctgcc cccgcaacca aaaaagtcat
       61 taaagccaat cacttagcgg tgattggctt ttttgcgtct ggagtgtcgt gcccacgcga
      121 tcgggtcttc gcgccctgag cgccgacatt catggctctt tgtgccacgt gccgccatgt
      181 tttcctgtca agtcagggtg ttgcccgcgt ggagcttttc actccccagt tctccgatcg
      241 agcaggtcgg ttcgcaggca gctggcggcg acgggcgagg cctgggcacg gcgtgcggat
      301 cggcactcgt tgccgatcgc gtggctggcg cgccggggtt gagagtccga ggagagtgcg
      361 ggtggtgcgg atgccctcga caggcttgcg accggcgggc tcgacgtctt gccgatcaag
      421 ccgatcacgc gcgacgcctt cgctcagcgg ccgcggtcct ccacggcggc gcgcagcgcg
      481 cggaagctct cgtcctcgcc ctggcccgag gtatcgacca catggtcggc gcgcaggtac
      541 tgcggttcac gcgcggcgag gatgcggcgc aggtcttcca tcgcttccgc gctaccggcc
      601 atcggacgca ggtcgccctg cgcgctgacg cgctgcatgt gctcctcggg cgaggtgcgg
      661 agccagatcg tcagacagcg cgacagcagc aggtcgtagg tctcggtctg cgagacgatt
      721 ccgccgcccg tggcgagcac cgtgcgttca ccgctgtcga gtgcgcgccg caggcaacgc
      781 tgttcgatgc ggcgatatcc cgactggccg tagagggcga agatctcgcc catgggcatg
      841 ccggtctcgc gttcgatctc ggcatcgagc tcgacgaaac ggaagccgag ttcggccgcc
      901 agcctgcggc ccagtgtggt cttgccggcg ccgcgcaggc cgatgagggc gatccggttg
      961 cgccgcgcgg cctcttcctg gccgtagtcg cgcatcaacc ggaagaccac gtcctccagc
     1021 cggtggcgcg gcagttgttc gaggatgcgc cgcaccaggc gctgctcgac cgactcctcg
     1081 gcgcccaggt tcaacatctc cggcagcgac agccccaatg cgctggcgat atggcgtagc
     1141 agcacgatcg agacgttgcc ctcgccggcc tcaagatggg cgagatgccg ctccgacacc
     1201 cccgcgtcgc gagccagctg cttgcgagtc aggccacgcc ggtcgcgcgt ctcgcgcacg
     1261 cgccggccga gcgcgagcag gaactccccg tcgctgcgcg gatcgggtcg caccgccggg
     1321 tgcgcgaccg gtacaggcgc gtcggaggct gcgagggtcg gattctggtc gttcattacg
     1381 gagcggaggt cggagattcg tgtttatggt gccgctggcg atgcgctgtc aacggcgtga
     1441 catcggaggc tcccggccgg tgtgcgggga gtgcattcaa gcatagcggc aagtgcagta
     1501 tattgcacct tagactttat ctaaataatt gaatttcctt gtttttggtg cgatgcaggg
     1561 ttgacagcat ccggattggg ggtattcttt tgcatgtctc gccgaaagat gcactttagt
     1621 gcactttttt ggatggcttg tgcggtaccc cagccagtcc gctcggcgat cgtgccaagg
     1681 ttctctcgcg tcgtaccgtt ccgtgtgcat gccggtcggt acggtccggg atcaggatgg
     1741 agacaggacg atgcacaccc tcagcaccgc agatcacggc tgcacgccgc cccggatcgt
     1801 gattccggat ccctacaacg ccgccgacga cctgctcggg cgcaacctcg tcgccgggcg
     1861 cggcagcaag gtcgcctaca tcgacgatgc cggcagctac acctacgacc agctcgcggc
     1921 gcgtgccaac cgctgtggca atgcgttgct cggcaccctc gggctggcac gcggcgaccg
     1981 cgtcctgatg tgcgtgctcg actgcatcga tttccccgcg atgttcctcg gcgcgatcaa
     2041 ggccggggtg gtgccgatcg cagtcaatac gctgctcacc gaacgcgact acgagtacat
     2101 gctctccgac agcggtgcgc gtgtggcggt ggtatcgcag gcgctgttgg ccaatttcgc
     2161 cgcgctgccc gggcgcgtgc cgacgctcga gcgcctggtc gtcgccggtg gtgacgacga
     2221 cgacggtctc gaggcgctgc tcgccgcgca gtccgagcag ctcgatgccg cgccgacgcg
     2281 tgcgaccgac ccctgtttct ggctctactc ctccggttcc accggtgcgc cgaagggcac
     2341 ggtgcacatt cactccaacc tcatccatac cgccgagctg tacggacagg aggtcctggg
     2401 catccgcgaa caggacgtgg tgttctcggc cgcgaagctc ttcttcgcct acggcctcgg
     2461 caacggcctc acctttccgc tttcggtggg cgccaccgcc atcctgatgg ccgagcggcc
     2521 cacgccggat gcggtgttca agcgcctgcg cgagcaccag cccgacatct tctacggcgt
     2581 gcccacgctc tacgccggca tgctcgcgca caccggctgc ccgaccgcgg gcgagatgaa
     2641 gcggctgcgc gcctgcacct ctgcgggcga ggcgctgccc gagggcgtgg ggcggcgctg
     2701 gaaggagcgc atcggggtcg acatcctcga cggcatcggc tccaccgaga tgctgcacat
     2761 cttcctgtcc aaccgcgccg gcgacgtgca gtacggcacc agcggcacgc cggtgccggg
     2821 ctaccgcgtg cgcctggtcg acgagggcgg cgacgagatc accgcggctg aggagccggg
     2881 cgagctgcag atctccgggc cgagctcggc cgtgcagtac tggaaccagc cggagaagag
     2941 ccgtgccacc ttcctcgagg gctggacgcg cagcggcgac aagtattacc gcaaccgcga
     3001 cggctactac gtctactccg ggcgcagcga cgacatgctc aaggtgagcg gcatctacgt
     3061 gtcgccgatc gaggtcgagt cggcgctgat cgcccacgag gcggtgctcg aggcggcggt
     3121 ggtcggcatc gaggacgagg aacgcctgat caagcccaag gctttcgtcg tgctcaagcc
     3181 cggctacgga cccggcgagg cgatgcggca ggagctgcag gcgcacgtca agggcatgct
     3241 cgcgccctac aagtatccgc gctggatcga attcgtcgag gatctgccca agaccgccac
     3301 cggcaagatc cagcgtttca agctgcgcgg catctagtgc tttggcggcg ctcgggggcg
     3361 ccgcccgtgt tcacaccatc aaaagagagg agacggacat ggtcggaaag gacaggaagc
     3421 tgcgcctcgc agtgagcgcg ctcgggctcg gcctgggcat gttgctggcg cagggcgcgg
     3481 cggaggcggc ggacaaggtc aaggtggggc tgatgctgcc ctacacgggc acctacgcct
     3541 cgctcggcaa cgcgatcacc aacggcttca agcagtacgt ggcggagcag ggcggcaagc
     3601 tcggcggtcg cgaggtcgag tatttcgtcg tcgatgacga gtccgatccg gccaaggcga
     3661 ccgagaatgc caacaagctg gtcaaacgcg acaacgtcga cgtgctggtc ggcaccgtgc
     3721 attcgggcgt ggcgctggcg atggccaagg tcgcgcgtga ctccaagacc ctgatgatca
     3781 tcccgaacgc gggcgcggac gagcttaccg gtccgctgtg cgcgccgaat gtgttccgca
     3841 cctcgttctc ggcctggcag ccggcgaatg cgatgggcaa ggtggtcgcc gaacgcggcc
     3901 acaagaacgt ggtcaccctg gcctggaagt actccttcgg cgaacagtcg gtcgccggct
     3961 tcaaggagtc cttcgagcag gccggcggca aggtggtcaa ggagctctac ctgcccttcc
     4021 cgaacgtgga gttccagccc ttcctgaccg agatcgccaa cctcaggccg gatgcggtgt
     4081 tcgtattctt ctccggcgcc ggggcggcga agttcgtcaa ggactacgag gcggcaggcc
     4141 tgaaggcggg cctcccgctc tacgccccgg gcttcctcac cgacggcacg ctggaggcca
     4201 tgggcggcgc cggcgagggc gtgctgacca cgctgcacta cgccgacggc ctcgacaacg
     4261 ccagggagaa gtccttccgc agcggctatg tcgcggccta caaggcgcaa cccgacgtct
     4321 tcgcggtgca gggctacgac agcgcgcagc tgctcgccgc ggggctctcg ggtgcgccgg
     4381 ccggagcctt cgacaaggag gcggtcatga aggcgatgag tgccgccacc atcgacagtc
     4441 cgcgcggtag cttcaccctg tccaaggcca acaacccggt gcaggacatc tacctgcgca
     4501 aggtcgaggg cgggcagaac aaggtgatcg gcgtcgccgc acccaagctc gccgacccgg
     4561 cgcgcggctg caagctcatg aactgattcc gtcgcggagg cggtggcggg ccctccgcct
     4621 ccgcgtcgca gcccgcttcc acccgtctgt ccatcgcatc ccggcgtgtt ccgcatgtct
     4681 gggagggaag ggttcaatca tggatttcac ctcgttcctg atccagatcc tgaacgcgct
     4741 ccagtacggc ctgctgcttt tcctggtggc gagcgggctg accctggtgt tcggcatcat
     4801 gggcatcatc aacctggcgc atggcagctt ctacatgatc ggcgcctacc tggccttcgc
     4861 gctcaccagc ctgaccggca acctgatgct cgcgatcgtg ctcggcatcc cgctcgcctt
     4921 cggcttcggc gcgctgctcg agaagctgct cttcgtgcat ctgtacaagc gtgaccacct
     4981 gcagcaggtg ctgctgacct acgggctcat cctgatcttc gaggagctgc gcagcctcac
     5041 ggtcggcgac gacgtccacg gcgtggccat ccccgacctg ttctcggctt cgatcgcgct
     5101 ctccgacaac ctctcctatc cggtgtaccg gctggtgatc tcggtggtgt gcatcgcgct
     5161 cgctgtcggc ttgtggtggc tgatgcagcg cacccgactg gggatgatga tccgcgccgg
     5221 ctcgttcaac cgcgagatgg tgcagtcgct cggggtgaac atcgacctgc tttaccgcac
     5281 cgtgttcgcg ctcggcgtgg cgctggccgc gctggccggc atgctcgcgg cgccgatctc
     5341 ctcggtgtac ccgggcatgg gcggcaatgt gctgatcatc tccttcgtgg tcgtggtgat
     5401 cggcggcatc ggctcggtgt ggggcgcgct ggtggcggcg ctgctggtgg ggttcgcgga
     5461 cacgttcggc acgatcctgc tccccgagct cgccggactg gcggtctatc tgctgatggc
     5521 ggtcgtgctg ctgtggcgcc ccgaaggcat cttcaggaag gggtgaggcg atgaacccgc
     5581 gagtcgtgct gtctttcaag ctcgccgtgc tggcagggct ggtgctgttt ccggcgatcg
     5641 gatccagctt ctatgtcgag ttggtggcga aaatcctcgt gatgtcgatc ttcgcgatga
     5701 gcctggcgct gctggtcggc ttcaccggcc tggtgagcct cggccatgcc gcatatttcg
     5761 gcatcgccgc ctacacggtc gcgctgatcg cgcccgaata cgaggcggcc aacctgtggt
     5821 tcgtgctcgc ggcctcggtg ctggcggcag gcgccgcggc gttcttcatc gggctgttcg
     5881 tgctgcgcac caagggcatc tatttcatca tggtgacgct ggccttcgcg cagatggcct
     5941 actacatctt ccacgacacc gcactcgggc gcagctcgga cggcatctat ctcaacgtca
     6001 agccggatgc cagcctcttc ggctggatgc cgttcaacct cgacgagccg ctgcacctgt
     6061 actacttcat cctcgccgcg atggtggcgg tgttcgtgct gctggccttc gtgctgcgct
     6121 cgcccttcgg tcgcgtgctc gccggcatcc acagcaacga gcaccgcatg aagtcgctcg
     6181 gctacgccac gcagcgctac aagcttgcgg ccttcaccct ggccggtgcg ctggccgggg
     6241 tggcgggctt cctgtacgcg gtgctgttcg gcttcgtgac ccccgattac ctgtcgtggc
     6301 atcagtcggg caacgtgttg ctgatggtca tcctcggcgg cctcggcagc ctggcggggg
     6361 cggtcgcggg cgcgttcgcc ttcatcggcc tgcaggagat gttctcggac tggaccaagc
     6421 actggcaact gctgatgggc ctggtgatcg tcgccgcggt gctgttcatg ccgagtggac
     6481 tggccggact gccgcgacgc atcttccgca cggtcaattg gggagaagac gatgagtgag
     6541 gcgatgctgg tggcggaccg cctgacccgc catttcggcg ggctgaccgc caacctcgag
     6601 gtgtgcatca cgctcgagcg cggcaagctg catgcgctgc tcggccccaa cggtgcgggc
     6661 aagtcgacct gcatcaacat gctctcgggc gacctgccgc cttccgcggg gcgcatcctg
     6721 ctcgaagggc aggacgtgac cgggctggat tcggcggcac gctcgcaccg cggcatcggc
     6781 cgcagctacc agcgcaccaa catctttccg caattcaccg tgctggagaa ctgccggctc
     6841 gccgcgcagt cgcgcaggcc gcggccgtgg cggatcttca ccgacgcgat gaagacgcgc
     6901 gacaccgtcg agcgcgcgca ggcggcgatc gtcgaggcgg gcctgggcgg gcgcgagctg
     6961 cgcaaggccg gcctgctctc gcacggcgag caacgccagc tcgagatcgc gatggtgctg
     7021 gccaccgacg cgcgtgtcct gctgctcgac gagccgctcg ccggcatggg ctcggaagag
     7081 tcggcgcaga tggtcgagct gttgcgcaaa ctcaagcaga accgcgcgat cctgctcgtg
     7141 gagcacgaca tggatgcggt gttcgcggtg gccgacacga tcaccgtgat ggtcgagggc
     7201 tcggtgctcg aatccggacc gcccgagcag atccgtaaca gcgaggacgt ccaggcggcc
     7261 tatctcgggg aggatgaaca tggctgagat gctgctcgaa gcgcgcgacc tccacaccta
     7321 ctacggctcg agccacatcc tgcacggggt gagcttctcg atccgcaagg gtgaggcgct
     7381 cggcctgatg gggcgcaacg gcatgggcaa gaccacgctg atccgcagca tgctcggcct
     7441 ggtgcggccg cgccacggca cggtgcgcgt gaacggcgac gacatgacgc gcgccaaacc
     7501 gcaccgcatc gcgctcgccg gggtggccta cgtacccgag gggcgcggca tctttcccaa
     7561 cctgagcgtg cgcgagaatc tcgtcatggc ggcgcgcgcg ggcgtggacg ggcggcgcga
     7621 atggaccttc gagcgcgtca tggagacctt cccgcgcctg ggcgagcgta tcgacaacgg
     7681 cggcggccag ctctcgggcg gcgagcagca gatgctcacg atcggacgcg cgctgatgac
     7741 caaccccgac ctgctgatcc tcgacgaggc caccgagggc ctcgcgccgc tgatggtcaa
     7801 ggagatctgg aaggtcatcc gcaccatccg cgacaccggc atcgccacgg tggtggtgga
     7861 caagaaccac tcggcggtga ctgcgctgac caaccgctcg gtgatcctgg tcaagggcga
     7921 ggtggtgttc gacggtccga gcgaacaggt gcgccaggac ccggccttga tccgcaagca
     7981 cctcggggtg tgatgcttcg atacgcgggc gcggcccggc aggccccgtc cgcgcgttgt
     8041 gcgccgcccg cggcggcgct gcgcccgtcc cggtccgtcc tcctcaatcg tccggcagca
     8101 ccgccgtgac ctcgatctcc accttcgcgc cggcctcgac gaagccagcc acctgcacgg
     8161 cgctcatcgc cgggtagtgg tggccgatca ggtcgcgata gaccgtgccg atctcctcga
     8221 ggcgggcgtt gtactcggcg gcgctgccca ggaaccaggt catgcgcacg atgtcggtcg
     8281 aacgcgcgcc gccggcgtcc aggatggcga gcacgttgcg cagcgcctgg cggatctgct
     8341 cgacgaggtc ctcgctgcgg aagacctcgt ccgcgtccca gcccacctgg ccggcgacga
     8401 acagcatccg tccgctcgcg gccactccat tggcgtagcc cttcggccgt ttccagcccg
     8461 cgggttgaag aatctgcatg tctgtctcct cgagtgcgtt tctggcgctg cgcgtacgcg
     8521 cttgatgcgc gaccgggcag catgtcaaat agtgcagttc gcggacgtat tctgcaataa
     8581 aatgcagaac ttgggcggct gacgatgcga tggtgtccgc tttggcggcg tgccgagcca
     8641 tagcgcaagt cttatagagg acttttgctt cgcacccttg acccacgccg cgatcgaaag
     8701 cattcttcaa aggttatgcg ccccttcatg cgggcgtgtt cctcaacaac cgagccagtg
     8761 agtgcgaaag atgaccgaac gagtgagtgt ccagcgtctg caggtcgcag cgaacctgaa
     8821 gcgtttcatt gaagaagaag ccctgccggg cagcggtgtc gacgctgccg ccttctggtc
     8881 cgggttcgat gcgctggtac acgatctcgc accgaagaac gccgctctgc tcgccgaacg
     8941 cgatcgcatc caggccgaga tcgacgcctg gcatcgcgcc cacccggggc cgatcgccga
     9001 catggccgcg tacaaggcct tcctgtcctc gatcggctac ctgctgccgg tgcccgccgg
     9061 tgccaaggcc accaccacca acgtcgatga cgagctcgcc gtgcaggccg gcccgcagct
     9121 cgtggtgccg gtgatgaacg cgcgctacgc gctcaacgcc gccaacgcgc gctggggctc
     9181 gctctacgac gcgctctacg gtaccgacgc catcccgcag aaggacggcg ccgagctcac
     9241 caagggctac aacccggtgc gcggcgcgaa ggtcatcgcc ttcggccgcc aggtgctcga
     9301 ccaggccgcg ccgctcgccg gtgcctcgca tgccgacgcc gcgggctacg cggtcgaggc
     9361 cggccgcctg gtggtgaagc tgcagagcgg tgccagcacc ggcctgcagc aggccgagaa
     9421 gttcgtcggc ttccagggcg aggccgccgc gccgcgcgcc gtgctgctga agaacaacgg
     9481 cttgcacatc gagatccaga tcgaccgcag cggcgccatc ggcaagtccg atgccgcggg
     9541 cgtgaacgac ctgctgatgg aagccgcgct gtcgaccatc atggactgcg aggactcggt
     9601 cgccgcggtg gatgccgacg acaaggtcgt ggtgtaccgc aactggctcg gcttgatgga
     9661 cggtacgctg gaagacacct tcgacaaggg cggcaagccg atgacgcggc gtctgaacgc
     9721 cgaccgcgag tacaccggtg ccgacggcaa gccggtaaag ctgcacggtc gcgcgctgct
     9781 cttcgtgcgc aacgtcggcc acctgatgac caacccggcc atcctttggg gcgagggcaa
     9841 ggaaatcccc gaaggcatca tggatgcggt ggtcaccacg ctgatcgcca agcgcgacct
     9901 cgagcgccgt ggcaactcgc gcaagggcag catctacatc gtcaagccca agatgcacgg
     9961 cccggcggag atcggcttcg ccgacgagct gttcacccgc gtcgagcagc tgctcgggct
    10021 gccggccaac accgtcaagc tcggcatcat ggacgaggag cgccgcacca gcgtcaacct
    10081 gcgtgcctgc atcgccgccg cgccggcgcg cgtggccttc atcaataccg gcttcctcga
    10141 tcgcaccggc gacgagatgc acacctcgat ggaggccggc gcgatgatgc gcaagggcga
    10201 catcaagaac agcaagtgga tcgccgccta cgagcgccgc aacgtgctca tcggtctggc
    10261 cgccggcctg cgcggccgcg cccagatcgg caagggcatg tgggccatgc ccgacctgat
    10321 ggccgcgatg ctcgagcaga agatcggcca tcccaaggcg ggcgccaaca ccgcctgggt
    10381 gccgtcgccg accgccgcca ccctgcatgc gctgcattac caccaggtca gcgtggccgc
    10441 ggtgcagcag gacatcgaga agctcgacct cgacaaggaa gccgaggcgc tgctcgacga
    10501 cctgctcacc atcccggtgg tggccaaggc cgagtggagc gagaccgagc gccagcagga
    10561 actcgacaac aactgccagg gcatcctcgg ctacgtggtg cgctgggtgg agcagggcgt
    10621 gggctgctcg aaggtgcccg acatcaacga catcggcctg atggaagacc gcgccacgct
    10681 gcgcatctcc agccagcaca tcgccaactg gctgcgccat ggcgtggtgc ccgccgagca
    10741 ggtcgaggcc acgctcgagc gcatggccgc ggtggtcgat ggccagaacg cgggcgaccc
    10801 gctgtaccgc ccgatggcgc cgaacttcga cgactcggtg gcctaccagg ccgcgcgcgc
    10861 gctgatcttc gagggctgcg cccagccgag cggctacacc gagccgctgc tgcaccgcta
    10921 tcgccagcag ttcaaggcca aggtcggcgc ctgagcgtcg tccacgctcg gcgcgagtcg
    10981 cgtcgctcct gtgctcctgc aggagcgacg cgactcgcga tccggcgatg gagagctgca
    11041 cgcgtgcatc gcggcgacgg cactggaaat gtaaaaaggc gcctcgcagg gcgccttttt
    11101 acatcttgct ccgcccgatc tgcatgatcg ggggatcaga ccctcaccac agctgccacc
    11161 acggccgcgt gtcttccggg ccaccgcgga aatacacgct gtcggggaag ttcttgcgca
    11221 tgacgcggtc ggcgtcgtcg cgcagctgcg ccatgccgag cttgtcgtag ctcatgacca
    11281 tcaggaacag cgcctcctcg atcgccggtg cctgcgggaa gttattcacc gcggtctgcg
    11341 cgcggttgat cgccgcgacg taggcgccgc ggttgtagta gtagcgcgag acatgcacct
    11401 cgtgcgaggc gagcgagttg atcaggtact gcatgcgcgc ccgcgagtcg tcggcgtagc
    11461 ggctctcggg gaagcgctcg accagttcgc ggaagctgtc gaaggcctcg cgtgcgccct
    11521 tggggtcgcg ctcggagaga tcctggcgcg acaggccggc gagcaggccg aggtcttcgt
    11581 tgaagttgac caggcccttg aggtagtagg cgtagtcgac gttcgggtga ttgggatgga
    11641 gcttgatgaa gcggtcggcc gcggcgagcg cgagcgcctg ctcgccctgc ttgtagtacg
    11701 cgtaggccac ctcaagctgg gcctgctggg cgaagcggcc gtagggatag cgtgcctcga
    11761 gcttctcgaa tagcgtcacc gcgcgatcgt agccgccctc gctcatcgag gccttggcct
    11821 cggaatacag cttttgtgcg ttccagccgg cggtctcgtc gacctgctcg ggcaggaggc
    11881 cgcagccgcc gagcagcaag gcgccaatca gcgcggcttt tgccgttaaa cttccggggg
    11941 tgaacctggc catcgaagac actcgcgtga atgaacacgc cgattatatc ccagccgccc
    12001 tcccagccga tcttcccgcc gacgacgccg atgcgtggga ggcgctgacg attcccgtcg
    12061 aactgggtgg gctgcgcctc gatcaggccc tggcacggct tctcccccaa cattcgcgca
    12121 gccgcctgca gggctggctc aaggagggct ggatccgcgt cgatggcgcg agccccgatg
    12181 cgcgccgcaa ggtgtgcggc ggggagtgcg tcgagatcga cgcgccgccg ccgcccgagg
    12241 tcaccgccgc gctgcccgag gacatcccgc tcgcggtggt ctacgaggac gaggacctgc
    12301 tggtgatcga caagcccgtc ggcctcgtcg tgcatcccgg cagcggcaac tggagcggga
    12361 ccctgctcaa cgccttgctg caccgcaacc ccgcgctcgc cggggtgccg cgggccggta
    12421 tcgtgcaccg gctcgacaag gacaccagcg ggctgatggt ggtggcgaag acgctggccg
    12481 cgcagaccga gctcgtgcgt cagctccagg cgcgcacggt cgggcgccac taccgcgcgc
    12541 tggtgcaggg cgcgctgacg gccggcggca cggtcgatgc gccgatcggg cgtcacccta
    12601 cgcagcgcac gcgcatggca gtcgtcggca acggccgtcc ggcggtcacg cattaccgcg
    12661 tcaccgagcg tttcccgcgt gcgaccctgg tcgaatgtcg tctggagacc gggcgtaccc
    12721 accagatccg cgtccacatg gcgcacatcg gccatgcgct ggtcggcgat ccgctttatg
    12781 gggcgcgccg ctgcggcgat gtgcgcctcg atgcgttccc gcgccaggcc ctgcatgcct
    12841 tccggctggg gctcacgcat ccgcgcagcg gcgaggcgat gcgttgggag gtgccgctgg
    12901 cggcggattt cgccgcgctg ctggacgagc tgcatggagc gccgcgatga gcctgccgcc
    12961 cggctaccgc tttttcgccc ctggcctgct gtgcccgcag tggagcctgc cgcccggggt
    13021 acgggccgtg gtgagcaccc gcgagggcgg cgcaagcttg ccgccgtggg cgagcttcaa
    13081 cctcggcagc catgttggcg atgatgcgcg cgcggtggcg gccaaccgcg cacgcctggg
    13141 tgcggcgatc ggcgccgagc cggtgtggct cgagcaggtg catggctgcg cggtggcgga
    13201 cgccgatcgc gcggccggag tgcccgaggc cgatgcggcc gtggcgcgca tgcgcgggcg
    13261 ggcgtgtgcg gtcatgatcg ccgactgcct gccagtgctt ttctgcgacg acgacgccac
    13321 cgtggtcgcc gccgcccacg cgggctggcg cggcctggcc gccggggtgc tggagcgcac
    13381 cgtggagcgc atgggcgtga cgccggggcg cgtgcgcgcg tggctcgggc ccgcgatcgg
    13441 gccgacggcc ttcgaggtcg gggacgaggt gcgcgcggcc ttcgtcggcg cggacccggg
    13501 cgcgaccgcc gccttcgtcg cgggtggcgt gccggggaag tggctggccg atctctgcgc
    13561 gctcgcgcgt cgacggctca cacttgcggg cgtggagcgg atcgacggcg gcagcgtgtg
    13621 caccgtggcc gatccggcgc atttctattc ttaccggcgt gatgggacga ccgggcgttt
    13681 cgccgcgctg atctggctgg aggaggggcg gcccggcgcg atcgcgtgag cgcggcgttg
    13741 ccgcgatccg tctcgcatga ccgtcttagg gattgcggct attgatcggc gtcagtttcg
    13801 gctctatcgt tgcgttcgag tgcttctcgc tcctgctgca ggcggcgacg tcggcgggcc
    13861 ggggtgccga acagccaaag aaacaaggcc aggggcgcga gcccccagaa cacgaaggtc
    13921 aggacggcgg caacgatcga atcctccgcc gcggcgacga ggacgacgac gtagagccag
    13981 gcgatcggga tcagatacat gggtggattc tagcgcgcgc cgcgagccgg cgccggagga
    14041 tcacggacag ggcaggaaac cgcggccccg gcccggagcg ggcggacgcg gcctcaggag
    14101 aaagggatgt cggatacgac gggcaagcag gtgccgccgg gaatgcaggc gatcctcgcc
    14161 gcgggacagg cgatggcgca gggtttcttc gatgcgctcg cacgccagca tgtggcgatg
    14221 caggattcct cgggggtggc ggcgcagaag ctgccgatgc ccgagaccga ggtgatggcc
    14281 tcgctgcaga aggtcttcgc cgagaagcac gccgaactgt ggtcgtccat gcttgcgcgc
    14341 acgccggggg cggcaggccc cgcggtggtg cagcccgagc cgggcgacaa gcgcttcgcg
    14401 gcgccggagt ggtccgagag cccggtgttc gactacatgc gccaagccta cctgctcaac
    14461 gccggcttcc tgcgccagat ggcggacgcg atgccgatcg ccgacggccg cgccaaggcg
    14521 cgcatgcagt tcctcacccg ccagtacatc gatgcgctgt cgccgagcaa cttcgcggcg
    14581 accaaccccg agttcatcaa gaccgcggtc gagaccaagg gcgagagcat cgcgcgcggc
    14641 atccagaacc tgctcggcga cctcgagaag gggcgcatct cgatgaccga cgacgcggcc
    14701 ttcgagatcg gccgtaacct cgcgctgacc ccgggttcgg tggtctacga gaacgagctg
    14761 atgcagctga tccagtacgc gccgctgacc gagaaggtcg cgcagacgcc gctgttgatc
    14821 gtgccgccgt gcatcaacaa gttctacatc atggacctgc agcccgagaa ctcgctcgtg
    14881 cgcttcgtcg tcgagcaggg cttcacggtc ttcctggtgt cgtggaagaa cccccgtccc
    14941 gacaccggcg gccattacac ctgggacgac tacctcgaca agggaccgct cgcggcgctc
    15001 gaggtggtgc gctcgatcac ccgcgtcaag aagcccaacg tgctcggctt ctgtgtcggc
    15061 ggcaccatcc tcacctcggc gctcgcggtg gcgcgcgcgc gcggcgagga gccggtggcg
    15121 agcctcacct tgatgaccac gctgctggat ttctccgacg ccggcgagct cggctgcctg
    15181 gtcgacgagg cgagcgtcac cgcgcgcgag gccgcgatcg gcaagggcgg ggtgctcaag
    15241 gggcaggaac tcgccaacgt gttttccttc ctgcgcgcca acgacctggt gtggcaatac
    15301 gtggtcggca actacctcaa gggcaacaag ccggtcgcct tcgacctgct gtactggaac
    15361 tccgattcga ccaacctgcc ggggcccttc ctcacctggt acctgcgcaa catgtacctc
    15421 gagaacaacc tgcgcgtgcc gggcaagctc aggatgctcg ggcacaaggt cgacctcggc
    15481 aaggtcgacg tgccggccta cctgatggcg gcgcgcgagg accacatcgt gccgtggaag
    15541 agcgcctacc tggcgcgcaa cgtgctgggc ggcgacacca ccttcgtgct cggcgcaagc
    15601 ggccacatcg ccggcgcgat caacccggcg tcgaagaatc gccgcagcta ctggaccgcc
    15661 gatgtgcgcc cggccgaccc cgacgaatgg ttcgagagcg cggccgagca caagggcagc
    15721 tggtggctgc actgggccga gtggctgcgc ggccatggcg gcaaggaggt ggccgcgcgc
    15781 ggacgcctgg gcagcacgaa gtacgagccg atcgagccgg cgccggggcg ctacgtcaag
    15841 gagcgttcct gacccggaac gaggaggttt tcagtgttcg acggcagcgg atcgggcggt
    15901 cgctgccgtc gtgttcaagg tccgtccagc actacgaaag taccccgcat agggagagga
    15961 gaagaagatg gcaagagttg cactggtaac cggcggcatg ggcggtctcg gcgaggcgat
    16021 ctgcatcaag ctggccgcgc tcggctacaa ggtggtgacg acccattccc cgggcaacac
    16081 caaggcccag gagtggctgc agaccatgaa caacatgggc tacggcttca aggccgttcc
    16141 ctgcgacgtg gcggatttcg actcgtgcaa ggcctgcgtc gagcaagtgg tcaaggaggt
    16201 cggtccggtc gacgtgctgg tcaacaacgc cggcatcacc cgcgacatga ccttcaagaa
    16261 gatgaccaag gccgactggg atgcggtcat cagcaccaac ctcgactccg ccttcaacat
    16321 gaccaagcag gtcatggacg gcatggtcga gcgcaagtgg ggccgcgtga tcaacgtgtc
    16381 ctcggtcaac ggccagaagg gcgccttcgg ccagaccaac tacgccgcgg ccaaggccgg
    16441 catgcacggt ttcaccaagg cgctggcgct ggaagtggcg cgtaacggcg tcaccgtcaa
    16501 caccatctcg ccgggctaca tcggcaccaa gatggtgatg gcgatcccgc aggagatcct
    16561 cgactccaag atcctgccgc agatcccggt gtctcgcctg ggcaagccgg aagagatcgc
    16621 cggcctggtg gcctacctgt cctccgacga ggcggccttc gtgaccggtg ccaacatctc
    16681 gatcaacggc ggccagcaca tgttctgacc cgtcggcggc cggttccggc tccgcaacgt
    16741 ggcgcgctcc ggtggagcgc gctttttttt gtgctcgcgg ggcgcgggaa tggggctatt
    16801 gcatcgcaac atgagttgtg aaacaggggc tataatccgc gcgtgaggtg gctgatgcgc
    16861 tgccgcatcc gcgaccgaag cgcaagccgg ctatcccggc ccgcaggcgg cctcggtttc
    16921 tacaaatgct cctgaaggat ttcaagatgt ccaagaaaat cgctcttgtc accggcgcca
    16981 tgggtggtct cggcacggcg atctgccagt cgctcgccaa ggacggcttc aaggtcgtgg
    17041 ccaactgcct gccgaacttc gagctaaagg acgcctggct gtccgctcag cgcgacctcg
    17101 gcttcgactt cgccgccgcc gaaggcgacg tgtcgaacta cgagtcgtgc gcggcgatgg
    17161 tcgccaagat cgaagccgac gtcggtccga tcgatgtgct ggtgaacaac gccggcatca
    17221 cccgcgacaa gttcttcccc aagatggaga agggccagtg ggatgcggtg atcaacacca
    17281 acctgaacag cctgttcaac gtcacccacc acgtctccgc gaagatggcc gagcgcggct
    17341 ggggtcgcat catcaacatc tcttcggtca atggcgtcaa gggtcaggcg ggccagacca
    17401 actactcgac cgccaaggcc ggcgtgctgg gcttcaccaa ggcgctcgcc gccgagctgg
    17461 cgaccaaggg tgtgacggtc aacgcgatcg ccccgggcta catcggtacc gacatggtga
    17521 tggcgatccg cgaagacatc cgccagggca tcatcgacac cgtgccgatg aagcgcctcg
    17581 gccgtccgga cgagatcggc gacctgtgcg cctacctcgc ctccgacaag gctgcctaca
    17641 tcaccggggc gaccatcaac atcaacggtg gcctgcacat gtgctgatgt tgcgccgtgt
    17701 catgcgcgaa atcacgcaac cccgtcgaaa gacggggttt tttcgtggtt tcgatgtcgc
    17761 agcgcgatat aattttttcg cacagagcga agacaccgcc gccgaggaga cggagaaatg
    17821 ccggaacagc agcgactgat caagaagtac ccgaaccgcc gtctgtacga cacgcggacc
    17881 agttcctaca tcaccctggc cgacgtcaag gacttggtgc tcggtcacga gcacttccag
    17941 gtggtcgatg cgaagaccgg cgaggacctg acgcgcagca tcctgctcca gatcatcctc
    18001 gaggaagagg cggggggcgc gccgatgttc accagcgacc tgctcgcgca catgatccgc
    18061 ttctacggca atgccatgca gggcatgatg ggcaagtacc tcgagagcaa catcaaggcc
    18121 ttcaccgaga tgcaggtcaa gctccaggac caggcgcgcg cgatctatgg cgagaacagc
    18181 ccggtgggcc aggacctgtg ggcgcagttc ctcaacttcc aggggcccgc gctgcagagc
    18241 atgatgggcg cctacgtcga ccagtcgaag aagatgttcg agcagatgca gacgcagctc
    18301 gagagccaga cgcgcaacat gttcaccggc ttccagttcc cgggctacgt ccgtcccgag
    18361 gacgccgaat ccgccgcgcc cgccgcgctc aagggcgacg gcaagaagtg agcggcgccg
    18421 cgcgccgctg aggccgccgc cgccccgacc cgacaggttc tttgcatgac caccttcaag
    18481 acttccgacc tgccgcgcgt cggcttcgtt tccctcggct gccccaaggc gacggtcgac
    18541 tccgagcaca tccttacccg gctgcgtgca gagggctatg cgatctcggg cagttacgac
    18601 gacgccgacc tggtcgtcgt caatacctgc ggcttcatcg atgccgcggt cgaggaatcg
    18661 ctcgacgcga tcggcgaggc gctcgccgag aacggcaagg tcatcgtcac cggctgcctc
    18721 ggcgccaagg acgatgtgat cctcgccgcg catccgcagg tgctcgcggt caccggcccg
    18781 cacgccaccg aggaggtcat gcacgcggtg catcgccacc tgcccaagcc gcacgacccg
    18841 ttcaccgacc tcgtgccgcc gcaaggcatc cgcctgacac cgcagcatta cgcctatctc
    18901 aagatctccg agggctgcaa ccaccgctgc agcttctgca tcatcccgtc gatgcgcggc
    18961 gacctcgtca gccgtccgat ccacgaggtc atgcgcgagg ccgaggcgct cgccgacgcg
    19021 ggcgtcaagg aactgctggt gatctcgcag gacacctcgg cctatggcgt ggacgtcaag
    19081 taccgcaccg gcttctgggg cgggcgcccg gtgaagacgc gcctgctcga cctggcgaag
    19141 gcgctcggcg aactcggcat ctggatccgg ctgcattacg tgtatccgta tccgagcgtg
    19201 gatgatctca tcccgctgat ggccgaaggc aagatcctgc cctacctcga cgtgcccttc
    19261 cagcacgcca gcccgcgtat cctcaaggcc atgaagcgcc cggccaatgc cgagaacgtg
    19321 ctcgagcgcg tgcgcaagtg gcgcagcatc tgccccgagc tgaccatccg ctcgaccttc
    19381 atcaccggct tccccggcga gacggaggaa gacttcgaga tgctgctgaa cttcctcgaa
    19441 gaggcccagc tcgaccgcgt cggcgccttc gcctattcgc cggtcgaggg cgcgaccgcc
    19501 aacgagctgc ccggcgcggt gccggacgag gtgcgcgagg aacgccgcgc gcgcctgatg
    19561 gacttccagg aggacatctc cacccagcgc ctcgaggcca agatcgaccg cgagatgacc
    19621 gtgctggtcg acgaggtcga cgaggagggc gcgctcgcgc gctcgccggg cgacgcgccc
    19681 gagatcgacg gcctggtcgt catccccgag ggcgacggcc tcgcgccggg cgacttcgtg
    19741 cgggtgcgca tcaccgactg cgatgtgcac gacctctacg ccgagcgcat cgagtaaggc
    19801 cggcatttcc gtgccggagc cctcgccgtg ctgagcgggg cgggctctgt cgggctggcc
    19861 tgcgccgagc cctcgggtgg cgggccggtg atcggcctgg cgctcggcag cggtgccgcg
    19921 cgcggctggg cgcacttggg cgtgttgctc gaacttgcgc gtgaaggcgt ggtgccgcag
    19981 atcatcaccg gctgctcgat cggtgccttc gtgggcgccg ccgcggcctc gggtgacctc
    20041 gacaagctgg tgcgctgggc cgagaccctc aagtggcagg acgtggtgtc gctgctcgac
    20101 gtgtcgctgc gcggggggct catcaagggc cagaagctga tcgacttctt cgagcgcaac
    20161 ttcgtcgacc gcgacttcac cgagctcgac cagcgcttcg cctgcgttgc caccgaactc
    20221 tccaccggac gcgagatctg gctgcacgag ggcagcgtgt cggccgcggt gcgtgcgtcg
    20281 atcgcgctcc ccgggctgat gacgccggtg ctgcaccagg gccgcatgtt ggtcgatggc
    20341 ggcctggtca atccggtgcc ggtgtcgctg tgccgcgcga tgggggccga cgtcgtgatc
    20401 gcggtggatc tcggctcgga catggtgggg cgcgccttcc ggcgctcggc ggtcgagcct
    20461 gcgccggtcg aggagaccga ggcggggtgg accgagcgcc tgctcgcgcg cttcggcttc
    20521 gccgccgaag ccgagtccgg catgccctcg cggcccctgg ctgcggccga cagccttccc
    20581 tcgctggtct cggtgatcag ctccagcatc aacatcatgc aggtgcgcat cgcgcgcagc
    20641 cgcctcgccg gcgagcccgc ggacctgctg gtgtcgccgc gggtgagcca gctcggcttg
    20701 atggactatc accgcgccga cgaggcgatc gccgagggcg tcgcggcggt ggcacgggtg
    20761 cgcccgttgt tgcgcgagct gctcggcggc gccaacgagt gaggatcgga tgagcgatga
    20821 actgaatgcg cgcctgcgcg cgatcgtcgg cgcggccaac gtgctcgacg gcgaaaccga
    20881 catggcaccc ttcctgagcg actggcgcgg gcgctaccac ggccgcgcgc gcgcggtggt
    20941 gcgtccgcgc gataccgccg aggtggccgc ggtggtcgcc gcctgcgcgc aggcgggcgt
    21001 ggcgatggtg ccgcagggcg gcaacaccgg cctgtgcggc ggcgcgaccc cgctcgccga
    21061 tggtgcggcg gtggtgatca gtctcgcgcg cctggaccgt gttcgtgcgc tcgaccccga
    21121 caacgacacg ctcaccgtcg aggccggttg cacgctcgcc gcggtacagg aggcggcgca
    21181 ggccgccggg cgcctgttcc cgctctcgct cgcctcggag ggcagctgcc tgatcggtgg
    21241 caacctgtcg accaacgccg gcggcgtgca ggtgctgcgc tacggcaaca cgcgcgacct
    21301 ggtgctcggg ctcgaggcgg tgctgcccga cggccgggtc tgggacgggc tgcgtgctct
    21361 gcgcaaggac aacaccggct acgacctcaa gcagctcttc atcggcgccg agggcaccct
    21421 gggcatcgtc accgccgcgg tgctgaagct cttcccggcc atccgcactc gcgccacggc
    21481 ctgggtggcg gtggccgacc cgcgcgcggc ggtgcgcctg ctcgggctct tgcgggcagc
    21541 ctgcggcgac cgcgtgagcg cgttcgagat tgtcggccgc acggcgctcg ggctcgtgct
    21601 ccggcacatc ccgggcgcgc gcgatccgct cgccggtgcg ccggcgtgga cggtgctggt
    21661 cgagctctcc gatccggccg tcgatgcgcc gctcgaggcc caactcgagg cggtgctggg
    21721 cgaggccgtc gcgcagggcc tggccagcga cgccgcggtg gcggcgagcg tggcgcaggc
    21781 gcgggccttg tgggcgctgc gcgaggacat ctccgaggcg cagcgcatcg agggcgtcag
    21841 catcaagcac gacgtctcgg tgccggtcag ccgcatcccc gagttcctcg aacgcgccgg
    21901 cgcggcgctt gccgcgcgct ggccggacat tcgcgtggtg gccttcgggc acatcggcga
    21961 cggcaacctg cactacaacc tgtccaaggc ggtggccgac gacaacgcca ccttcattgc
    22021 acgcaccgca gaggtcaatg gcatcgtgca cgacctggtt tgcgaactcg gcggctcgat
    22081 ctctgccgag cacggtctcg gccagctcaa gcgcgaggag gtgctgcgct acaagccggc
    22141 gctcgagatg gagctgatgc ggcgggtgaa gcaggccttc gatccggccg gtttgatgaa
    22201 tccgggcaag gtgctgtaga aagttcggag agggggttta caggtctcgg ggagcgacct
    22261 ataatgcgcg ctctttggtg ccgggggtat agctcagctg ggagagcgct tgcatggcat
    22321 gcaagaggtc agcggttcga tcccgcttac ctccaccaaa ctgaagaatg cagtccccat
    22381 cgtctagagg cctaggacac cgccctttca cggcgataac cggggttcga atccccgtgg
    22441 ggacgccata caaggcggtc cggccaatgg ctggaaagca aaccggtgaa agccgggcag
    22501 gtcagcgcgg agtggtagtt cagtcggtta gaataccggc ctgtcacgcc gggggtcgcg
    22561 ggttcgagtc ccgtccactc cgccaccaaa aaagccaccg tacggtttcc gtgcggtggc
    22621 ttttttccgt ctgcggccgc aacgggcatg cttccgttgg tcctcgggga ggggcccgca
    22681 tgcgcccctc ctcgcgcctt acgccccctg cttcttgatg cggtagttca gctgcggccg
    22741 cgtcatgccc agcagtcgcg ccgccgccga caggttgccg ttggcgcgct cgaccgcgtg
    22801 ctgcagcagg cgctgctcga aggcgtcgag cgacatcccc gagtcgagta ccgccgcggc
    22861 gagatcctgc tgcgcgcgtc gcgcggtttc gagcccgccc tgggcgctga gcgagtgctc
    22921 gcgggcgtgc tcgtcgagcg tgccgatgaa gaggtgctcg acctcgatct ggccgttctg
    22981 gggcgcgagg atcacgccgc gctcgagcac gttctcgagc tcgcgcacgt tgcccggcca
    23041 ggcgtgcagc ttgagtgcgt gcatcgcctt gtcgctgagg ccggcgatgc gcttgccgtg
    23101 cagggtttcg aagcggcgca ccatggcctc gaccagtgcg ggaatgtcgc cggtgcgctc
    23161 gcgcagcggc gggatggtga ccggatagac gttgaggcgg tagtacaggt cgcgccggaa
    23221 agtgccggca ttgaccgcct tctgcaggtc gacgttggtc gcggcgacga ggcggacgtt
    23281 gaccttgcgc gtgcgctcgt cgccgaggcg ctcgatctcg ccttcctgca gcgcgcgcag
    23341 caatttggcc tgcgcgggca gcggcagctc gccgacctcg tcgaggaaga gggtgccgcc
    23401 atgcgcgcgt tcgaacttgc ccgcgcgcga ggtgtgcgcg ccggtgaagg cgccgcgttc
    23461 gacgccgaag agctcggatt cgatcagctc gtcgggcagc gcggcgcagt tgaccgcgac
    23521 gaaggcctcg tccttgcgcg cgctcatctc gtgcagggta cgggcgaagc gctccttgcc
    23581 gacgccggtc tcgccgagca gcaacaccga cacgttcgtg gacgcggcgc gaccgatgag
    23641 gtcgtaggcg tgcaggaagg cggcggagct gccgatgagg tcgggggtgc ggcgcggaca
    23701 ggagatcgtg cggcgcatgg cctcgatctc ctcacgcagc tcgagcaggc ggccgacgat
    23761 cgagtcggcc tggaagtagg gcaggagggt gtccgcgtcc ggccagtcct cggccggccg
    23821 gccgacgatg cggcagtgct cgtgcccggt cgcggcgcat tcggtctcgc ggaagaggat
    23881 gaagcgtccg gtgaaggcgc tggtgtagcc ggaagcgtag ccgatcatca tccagcacac
    23941 cggctcggcg gactcgccga aggcctgcac gtgcgcttc
//
LOCUS       DAOQBS010000038        23670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1386_length_23670_cov_9.756638, whole genome shotgun sequence.
ACCESSION   DAOQBS010000038 DAOQBS010000000
VERSION     DAOQBS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 23670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23670
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1386_length_23670_cov_9.756638"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKN25_06315"
     CDS             complement(<1..233)
                     /locus_tag="PKN25_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNV90546.1"
                     /translation="MRFRPIAVALLTATLTLPAMAQQAPAPRDVLTHYAELVHAGYAD
                     SAASARTLKLAIDAFTRAPSEEGLREARARWLAA"
     gene            complement(364..807)
                     /locus_tag="PKN25_06320"
     CDS             complement(364..807)
                     /locus_tag="PKN25_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90547.1"
                     /translation="MKQPEPTGWHRRKYWLNALVLLAPIWFLYASLTPVFPPPWETKT
                     AGQITVTPSPHDEGAPYEHDGIRVKDFSFRFCDGCRETIRLASANVGLAPAAPTAVED
                     GLVHGQGNLQEAHVPYPARLSGNERLWLSVQTWDGALHHIDWPLR"
     gene            complement(804..2054)
                     /locus_tag="PKN25_06325"
     CDS             complement(804..2054)
                     /locus_tag="PKN25_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNV90548.1"
                     /translation="MHAATASSATFARWSVRPAIGSDPMAIGNRKRWFQVHGWLSLPV
                     WAVFCFVCVTGTIAVISHELTWLTNPAARATNPDDRPAQPLPVLVDAVRDAVPGAQIR
                     QVMVFESYLVTAIGFSVPGKPPALAYVNPYTAEVQAINEGLTFIGFMRSLHGWLLFPW
                     HHSWSLGYYLVAAMSLVTLGAMTTGVVIYKRFWRAYTRPQLRVGAGARVLLGDFHRLA
                     GAWSLWFLLVIGLTGLWYLVQAMLWHNEVHVWDEPALVAGQDLPQAVGTPPEPIGLER
                     ALGAANAALPEMRPRWISFPEHNRAHFSIAGTSDNFLYDQYSMRAFVNPWTGTVDATR
                     TPDDMNALQTIAHIADPLHYGTLGGLWTKLIWFVFGLLLSTMSITGFVIWRKRTFGAA
                     TRDGRVKEAGGEVPARSGSTLENA"
     gene            complement(1987..4155)
                     /locus_tag="PKN25_06330"
     CDS             complement(1987..4155)
                     /locus_tag="PKN25_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNV90549.1"
                     /translation="MITLPSRHAHLVRTSVANAPGCSLASSLIALAFGLGTSAAIAQD
                     TSRTTTLPAVTVTDGVTESATGPVLGYVAKRSATATKTDTPLSETPQAVSVITADRMR
                     DQGAQTIQDALRYTAGVRGETYGLDSRGDWSTVRGTDPVVFQDGMQQTFGYYASARPD
                     PFTLERIEVLKGPASVLYGQGSVGGIINLVTKRPLDTRHGEIQLQAGNYDRAQLAADI
                     TGPLNEDKTLLYRLVAIGRDSGTQVDKVDDDRYVLMPSLTWRPDAQLEWTLLANLQKD
                     ETGSTTQFLPHAGTVLPAPAGLPRIPVDVFMSEPGFDEYDTEQRALTSLLTYRPNDVW
                     TLRQNLRYADSEVSYQTIYPRFRPQLQPNGDIDRVFWVAKPDLKYWTVDHQAQANLDQ
                     GRLKQTLLAGIDFQHAVTNRRWAYGAAGTLNLYNPVYGNFTPPAASAFVDDPENTLRQ
                     TGFYVQDQLTWDERWIVVLGLRHDRAENATEGNPTQRDDANTKRLALMYKADNGLSPY
                     LSYAESFEPTIGLNGDGKAFKPLRGEQYELGLKYEPAGGFGLINAAIYDLREENRKAP
                     DPNNPLNQLQTGEARTRGLELEAVLHVARNWDLIATYSYTDSEVLEGTNAGKNLPSVP
                     EHMASLWSQHRFSIGEMTGFRGGFGVRYIGTSWDGTDTLATPSQTLVDAMVGYDVGAW
                     SFALNVNNLTDETYYTTCLARGDCFVGNLRTVVGTASYRF"
     gene            4675..5745
                     /locus_tag="PKN25_06335"
     CDS             4675..5745
                     /locus_tag="PKN25_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNV90550.1"
                     /translation="MEPADTAHRTTYAIKRKHSQVDATPTPDLFDSLERWLSTPELAY
                     ASWLHPQPLRVSTKTVYTAMFGRFCQWLSAQGRRLDQLEADDIRRFLDSAGSAPRQRA
                     QSGRQRQQYVRQLERVFAHLGALGYGGNNVGRMAGLERVGQGDDQPGRFLTSAECEAL
                     IARLKTRLADLERERKGAEAWIEYRDLALVAVMLGAGLKVRDVVGLTLNCINRGEERI
                     ELSRPGCAHRARILAFAVSPIQAWLALQDQIHGNAAASPMRKVFEADRSVGFGRLSKQ
                     VTLSASSVHRRTQKLLEEAGITGERACAQTLRNTYAGLLIEGGASDEQLIDYLGLQAS
                     ISAQRLRASFARAKAPPAQRQG"
     gene            6063..7505
                     /locus_tag="PKN25_06340"
     CDS             6063..7505
                     /locus_tag="PKN25_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90551.1"
                     /translation="MTPEQEKQLLQALYDRLFDAITYVPQGGGVNPFTKNETFIHFTK
                     NETLQPSAFADAWNPTNPNADLGASELFATMVDKVAPLSLEWAPKDQPLALVYKSIVD
                     GMNEAKSARPSQASIDAYNKAKAYLQKSEKNPFTGEMTAGGLSDEYQAYLKNLQALTE
                     AYSDYTSRYNTFVDEVVDAEAANDANAKKRAQREWVADEKGLRREISNAQANLTAGNS
                     KWVQMALDTMNTTINDAIARALESAKAQVADDQFNAGTQNKWLLTYANPTNWYDPSAT
                     ENFSELTISSKNKVDDKSTTSHAYTFGASFNAGLWGVSAKSQGEFSNTQHHMKADEIS
                     IRCKIAKVSIVRPWFDETLFRSDNWFTNLRGEDETLYISNGKLDSTNADSILPMYPVA
                     FIVARDIEITANFTKEDLEIIKQSASAETKVSYGPFSIGGSYKYGNEKEHSELEVSDG
                     KLRVPGMQIIGWVSRVMPPSPKRSRKVLDT"
     gene            7715..8884
                     /locus_tag="PKN25_06345"
     CDS             7715..8884
                     /locus_tag="PKN25_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90552.1"
                     /translation="MLQLNAIPVSIDLEKFSDAYSSDNPGGDYEAAYRLRNLCNAIPS
                     FKRNFDPSGHYVEAIWRNIAFAATGTTSYAQHLLSVAQADIDGAELSNLGGSPKPWLP
                     VDAAPSNWTELLADAPECELDLGGDGGSGDYVLIDQAENLAWTGMPGAEATPIEGKLQ
                     RIRLRALRVDLNRSWLDLQLLAISGWKINGMPSGFFSSGTQADNSGIFPLLPTALVIG
                     TDIIIEGDWSRADLKLMGRHAAEGRALGIGPFPLAAPALAVGAPLQIQQAHVIGVISA
                     LVPYAPQATDVDPGLVLVKNDGGFIARFAVDWRLSGHPQRSESGSFPVVAAKSVSLPA
                     GATDIAVTIEIMTFPPPFETWKVLTVRNYDTAPRVSFRLSGTTIDTVIEELPVFG"
     gene            9015..12113
                     /locus_tag="PKN25_06350"
     CDS             9015..12113
                     /locus_tag="PKN25_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90553.1"
                     /translation="MASIVAGEEGAQVTLAAQTDATFTVKAAPVHPVQVLENSYQRAI
                     REASFREPDPGKRQAVERLALARYFPFAAGELELVAAPDGAAELVDARFEAGRAAISQ
                     VRELAQGRVNDGAGGTADDLEAGKGLCREALGICETLLGDYDAIGGEMGYDVRQIPII
                     RELHDMLGTGTAELTVAKDWAAMAAVCSGTLRRLLLRFSQLMEGLACVQVFHVIDTFY
                     LYRLLVRYRVEGDQEPGDDLRMAVSGGLTGLLNNGVPVNQDRVWLSDWLKAKMREIDV
                     ALQRIGNVHFHLKGGRALEYVRRLPEEGRNDWDTSVLIDPDLSADAWYAKFAEIHNDL
                     VPRLQRFKREFFVLVHKHAGAINASLDAYVPKVKPKEEVVRDGDHAEEDDFEAGARDY
                     DVMDGALSDRAERVRNEGDDGARGAPGGIPFPKFAAACKAELIDIGIPRRDTVDAIAH
                     WHHIGPGILRKPWTEQIPVPGHRYYIDEYLMMIRDAFETPAKKMEKRISRLANFLSLG
                     PEDNDPAETIEVAVAHARAEAEAAGLAKSLAVVDAQSVPVKRLMYVMLEEIASSHELR
                     SRPALGGVIDEILATQASDRALRAGLPATYPAAIADALPALSEPHKEVLSWIALMHAI
                     SRRFETHLADKAAYFGFCPQLATGAPAVSADEKARGDAIVALLRWLHGESAFRPDKEH
                     EVQFAITGAYACALHTDYARNRLPAALKQQLQIGLDPVEVVDVKLFCHPDPRRAIDPA
                     TVLEALVRPLVEEYAATKNPPFAFQFHPDRIDVVWKDEVKIRDVSYRPLVARIRVVKE
                     GWPLLAYVWGLPVLSLADLIREYFSRASLHQDFLAEKRLKQTAALLTSLLTYFEANDA
                     IDPNAVIVDPTNDVDAVLNEITRVEAMTKSNDKSVALQGIARRAPYDDRIVARILQAC
                     GTLASSQVAPVLFSLLDREDLTTQGYVAIACGAKAVTSSSEREGLLMRYAEVAPLVDR
                     VFAALLDATASMKAAEKIADVLIVVAKRRDVTTEIKARLREFAQAFVNVTRHRNRVIE
                     AL"
     gene            complement(12563..>12926)
                     /locus_tag="PKN25_06355"
     CDS             complement(12563..>12926)
                     /locus_tag="PKN25_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90554.1"
                     /translation="ELAPITPPIYRAHLRAIKALEDAQQDSEGAKLTDQQVAALKGAL
                     LSDAQLEALARMKSHTDFNDLATRSVLGREGVERQVKAEAVRVIRKIEAQRERPPEQE
                     KEHNSHAPLPPKRVARIA"
     assembly_gap    12927..13026
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13027..15917)
                     /locus_tag="PKN25_06360"
     CDS             complement(<13027..15917)
                     /locus_tag="PKN25_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HNV90555.1"
                     /translation="MAERRKPFHETVAERLIEQLKAGTAPWQRPWVPGEPGSMLPVNP
                     TTGKRYKGINAIHLMSQGRTDPRWMTYKQAAAAGAQVRKGEHGTAVQYWKFSEEQALR
                     DEHGRPVLDGEGRPAQVTVVLERPRVFFATVFNAEQIDGLVPLERQPPAWDALERAEH
                     ILQASGAVIRHGEIDQAFYRLATDSIHLPDKGQFETASNYYATALHELGHWTGHATRL
                     DRDLAHPRGSEGYAREELRAEIASMILGDELGIGHDPGQHVAYVASWIKALQADPLEV
                     FRAAAEAEKIKDYVMAFEQRQVQEHGPQQEPDQAPLLPPPPTVAELSAAQYAAMKAAD
                     AAFGAALVRAYGETAAGDRRYAPSHDDPDVQAAADAFKSAADAWREAVADARHIVAGR
                     DSVQQAIPTEEVSMQTHTDDATDPREHAARVEAWALAHLMRDTLPHALASARLEQIGR
                     ARALLDEMQPLNTQNAFWTRHALPDDIDALDARIQGVIAPLEQLREEAKVAQARQAGD
                     RAAFEAAAREAFDIALPHDWNGQVQVQASAELDFEGATHVVAATDLGVAPQFWSVYVQ
                     RDDGRYAFVQDFRHAQQAEHLAYRLAVVAANSTDSAQAREAILARAHEDRVRRDPAST
                     EADIAAASEARKTAEMNRTLADAEAQTHGAKAAVQQDSPSAQPAEAARTYLHVPYTEK
                     DAAKALGARWDRKEQAWYVPPGVDAAPFAAWMQGGARAPVPAQTREDPGARRRVYLAV
                     PYGERGAAKAAGAAWDAAAKSWYAGPKADMERLHRWLPDKMPAEQAPAMNPRDEFAEA
                     LRSIGAIVTGEHPIMDGNKHRIAVEGDRKGEQAGFYVVHLDGHPAGYIKNNRTGVEMT
                     WKAKGYVLDPQEKARLAAAAASTLAARADAQARQHEASAQRVCRQMAELVSVTEPTPY
                     LRDKGIRVHAGVFTDRDGKTTCIPAFDVDGKQWTMQYIQEDGTKRFAKD"
     gene            complement(15982..16101)
                     /locus_tag="PKN25_06365"
                     /pseudo
     CDS             complement(15982..16101)
                     /locus_tag="PKN25_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122510.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
     gene            complement(16256..16792)
                     /gene="traF"
                     /locus_tag="PKN25_06370"
     CDS             complement(16256..16792)
                     /gene="traF"
                     /locus_tag="PKN25_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer signal peptidase TraF"
                     /protein_id="HNV90556.1"
                     /translation="MSGRFARLAAGVALAGAAAMAVAALAQAAGARINTTRSVPLGLY
                     WVSDAAAEKGAYVLVCPPQGPVFDEARARGYIGAGFCAGGYGYLIKRIAATGGDAVAV
                     SEAGVRVNGALLALSAPRAVDAGGRALPGFRRDATVLGAGEVLLMADASPTSFDARYF
                     GPVSRGQILNVVVPLLTW"
     gene            complement(16969..18870)
                     /locus_tag="PKN25_06375"
     CDS             complement(16969..18870)
                     /locus_tag="PKN25_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HNV90557.1"
                     /translation="MKINMNNAVGPQVRARKASGSRVLSALGALSLAGGLQSATQFFA
                     HTFDYQAQLGRHVHHVYAPWSILEWSSTWYARYPDEVMTAASVGLLVATVGLLGVAVA
                     KVVTSNSSRANAYLHGSARWAEKKDIEAAGLLPRPRTWLEVVTGKTAARAAGVYVGGW
                     EDEDGTFHYLRHNGPEHVLTYAPTRSGKGVGLIVPTLLSWGASAVVTDLKGELWAITA
                     GWRKTHARNTVLRFEPAAADGSVCWNPLAEIRLDTEYAVGDVQNLVTLIVDPNGKGLD
                     SHWQKTAFALLVGVILHALYKAQDEGSAATLPAVDAMLANPNRDIAELWMEMATYGHL
                     DGQNHPAVGSAARDMMDRPEEEAGSVLSTAKSYLALYRDPVVARNVSRSEFRIRDLMN
                     ADDPVTLYIVTQPNDKDRLRPLVRVMVNMVVRGLADRMDFEHGRPVAHYKHRLLLMLD
                     EFPSLGRLQILQESLGFVAGYGIKCYLICQDLNQLKSRETGYGPDETITSNCHVQNAY
                     PPNRVETAEHLSRLTGQTTVVKEQITTSGRRTAAMLGQVSRTFQEVQRPLLTPDECLR
                     MPGPRKDAEGHIEAAGDMVVYVAGYPAIYGKQPLYFKDPVFRARAAVPAPTVSDRLIA
                     FADAGERGA"
     gene            complement(18867..>20322)
                     /locus_tag="PKN25_06380"
     CDS             complement(18867..>20322)
                     /locus_tag="PKN25_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraI"
                     /protein_id="HNV90558.1"
                     /translation="RRHYDKAPLPLRVDTAALYARYQAEQQRRSAERTDALASARQRK
                     ARAIEAARRANRLWRATIKVADGKGIDKRLLYAQASRALRAQLAAIHAAYADAREALY
                     GGFQRRAWADWLKHEALHGNAEALAALRAREVARGLQGNTVTGGGGRRSGPSSVPDPL
                     RNEPLVRDTITKKGTILYRAGASAVRDDGDRLQVSDEATREGVQIALRLALTRFGPRL
                     TVSGTPAFKAQVIRVAVDLQLPLTFTDPALELRRQQLSSKEPGHEHALHVRRDGAERG
                     RADRRRPRGAGDGATVGAPRRGGRDERGGGDAPALRQPDPGRPGRVPPPHCRHRLRAL
                     SELGVVRFADGAEVLLPGDVPRHLEQPGPQPDHALRRDLPGAGGRDAPRLTPAQRAAA
                     DTYIAERNAKRVQGIGIPKHALYNGCAGVMIFAGLRRIDEEALALLRHGEADEIQVLP
                     VDAATARRLSRVALGEALTVTPRGTMAMSKGRSR"
     assembly_gap    20323..20422
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20423..21205)
                     /locus_tag="PKN25_06385"
     CDS             complement(<20423..21205)
                     /locus_tag="PKN25_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HNV90559.1"
                     /translation="MIAKHVPMRAAACSDFAGLAAYITDEQDTAHRLGQITVTNCAAA
                     SLQAVIGEVLATQRQNTRAGGDKTFHLLVSFRAGEALGAATLTAIEARLCAGLGYGDH
                     QRVSAVHRDTDNLHLHIAINKIHPERHTMHEPFQSYRTLAALCAALEREYGLQPDNHL
                     PRRSVAEGRAADMECHAGIESLLGWVRRHCLEALRAAPSWAALHQVLHENGLVLRARG
                     NGLVVEASDGTRVKASTLGRELSRPSLEARLGPFEAPPDGPRP"
     gene            complement(21240..21614)
                     /gene="traJ"
                     /locus_tag="PKN25_06390"
     CDS             complement(21240..21614)
                     /gene="traJ"
                     /locus_tag="PKN25_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HNV90560.1"
                     /translation="MEHDPTPSARRRTQHLRVPVFADEKALIEAQAQRAGMSVARFLR
                     EVGQGYRIDGVVDYEQVRELARINGDLGRLGGLLKLWLTNDERTAQFGEATLRAVLAR
                     IEATQDEMGQVMMKVVMPRAQR"
     gene            22000..22392
                     /locus_tag="PKN25_06395"
     CDS             22000..22392
                     /locus_tag="PKN25_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HNV90561.1"
                     /translation="MASRYADELAEWVNARPAPRPRQDRSLVAFLAVKEDVRAALEAG
                     YAMKTIWAHLQATGRIESRYETFTLHVKRHLRTPPPAQPPTAAQAQRPPAGPAATSPP
                     APREPRKTLPPSVGGFTFNATPKTEDLF"
     gene            22392..23117
                     /locus_tag="PKN25_06400"
     CDS             22392..23117
                     /locus_tag="PKN25_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011304098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HNV90562.1"
                     /translation="MAKIHMVLQGKGGVGKSMIAAVLAQYKAGKGNTPICIDTDPVNA
                     TFAGYRALDVRRLKLLDGDEINTRHFDALIEQIATAAADVIIDNGASSFVPLSHYLIS
                     NQVPALLQELGHELIMHTVITGGQALQDTVSGFAQLAGQFPAQCQFVTWLNPYWGAIE
                     HEGKGFEQMKAYVAHKARVSAIIQVPPLKDETYGRDFADMLQARLTFDEALALSSLTI
                     MTRQRLKIVKTQLFAQLDNAAVL"
     gene            23117..23551
                     /locus_tag="PKN25_06405"
     CDS             23117..23551
                     /locus_tag="PKN25_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraM"
                     /protein_id="HNV90563.1"
                     /translation="MSDRIEALVKEIAAKHGIAVGRDDPIMILQTINERLIQDSAAAQ
                     QDALDRFKEELESIAHRWGEDARAKAERTLTTALAAGRAAMSKDMQAGAKAAALSARQ
                     EIDAALAQLADPIRDGRRIAILNLIAAGMAVFAAALALLSTL"
BASE COUNT         3849 a         7870 c         7923 g         3828 t
ORIGIN      
        1 gccgcgagcc agcgtgcgcg cgcttcgcgc agcccctcct cgctgggtgc cctggtgaac
       61 gcatcgatgg ccagcttcag cgtccgggcg gatgccgccg agtccgcgta ccccgcatgg
      121 acgagctcag cgtagtgcgt cagcacgtcc cgtggggcag gggcctgctg cgccatggcg
      181 gggagtgtca gcgttgccgt gagcagggct acggcaatgg ggcgaaagcg catcgtgaat
      241 ctcctgtttc agaggtgtgg ctccggaggg cgggctgcag tgccttggga gcggcaagta
      301 gtggggcatt ggccttgcat ggttggcgcg ggcgaggccc cgatccgagc ggatcgcgcg
      361 agctcaacgc agcggccagt cgatgtggtg cagcgcgccg tcccaggtct gaacgctgag
      421 ccacagccgc tcgttcccgc tcaagcgcgc cgggtagggg acgtgggcct cctgcagatt
      481 gccctgaccg tgcacgaggc cgtcctcgac ggcggtcggg gcggcgggcg caaggccgac
      541 gttggccgag gcaagccgga tggtttcgcg gcagccgtcg cagaagcgga aactaaagtc
      601 cttcacccgg attccgtcat gttcgtaggg cgcgccctcg tcgtgcggcg aaggcgtcac
      661 cgtgatctgg ccggcggttt tcgtttccca tgggggcgga aacaccggcg tcaatgaggc
      721 atacaggaac cagatcggtg cgagcaggac cagcgcattg agccagtatt tgcggcgatg
      781 ccagccggtg ggctcgggct gtttcatgcg ttctccaggg tcgaacccga gcgggccggg
      841 acctcgccac cggcttcctt cacccggccg tcacgggtgg ccgcgccgaa ggtgcgcttg
      901 cgccagatca cgaagcccgt gatcgacatc gtgctgagca acagcccgaa cacgaaccag
      961 atcagcttgg tccagaggcc gcccagggtt ccgtagtgca gcgggtcggc aatgtgcgcg
     1021 atggtctgga gcgcgttcat gtcgtcgggt gtccgcgtcg catccaccgt cccggtccat
     1081 gggttcacga aggcacgcat cgaatactgg tcgtacagga agttgtcact cgtgccggca
     1141 atgctgaagt gggcccggtt gtgttccggg aacgagatcc agcgcggacg catctcgggg
     1201 agcgccgcgt ttgcggcgcc cagtgcgcgc tcgagcccga tcggctcggg cggcgttccg
     1261 acggcctgcg ggagatcctg cccggccacg agcgccggtt cgtcccacac gtgcacctcg
     1321 ttgtgccaga gcatggcctg cacgaggtac cacagcccgg tgagcccgat cacgagcagg
     1381 aaccacagcg accaggcgcc cgccaggcga tggaagtcac cgagcagaac tcgcgcgccc
     1441 gcgccgaccc tgagctgggg gcgggtgtag gcgcgccaga aacgcttgta gatgaccacg
     1501 ccggtcgtca tcgcgcccaa ggtcaccagc gacatcgcag cgaccaggta atagccgagg
     1561 ctccagctgt gatgccaggg aaagagcagc cagccgtgga gcgagcgcat gaagccgatg
     1621 aaggtcagcc cttcgttgat cgcctgcacc tcggcggtgt acgggttcac ataggcgagt
     1681 gcgggcggct tgcctggcac ggagaagccg atggcggtga cgagatagga ctcgaacacc
     1741 atcacctggc ggatctgcgc cccgggcacg gcatcgcgca ccgcgtcgac cagcacgggc
     1801 agcggctgcg cgggccggtc gtccgggttc gttgcgcggg ccgccgggtt ggtgagccag
     1861 gtcagctcat gactgatcac ggcgatcgtg cccgtcacgc agacgaagca gaacaccgcc
     1921 cacaccggca gcgacagcca gccgtgaacc tggaaccagc gttttcggtt tccgatcgcc
     1981 atggggtcag aaccgatagc tggccgtacc gaccaccgtg cgaaggttgc cgacgaagca
     2041 gtcgccgcgt gcaaggcagg tcgtatagta ggtctcgtcg gtgaggttgt tgacgttcag
     2101 cgcaaagctc caggcgccga cgtcatagcc caccatggca tcgaccaggg tctggctcgg
     2161 tgtggcgagc gtgtccgtgc catcccagct ggtgccgatg tagcgcacgc cgaacccgcc
     2221 ccggaaaccc gtcatctctc cgatcgagaa gcggtgctgc gaccacaacg aggccatgtg
     2281 ctcgggcacg ctgggcaggt ttttgccggc gttggtgccc tcgagcacct cactgtcggt
     2341 gtatgagtag gtggcgatca gatcccagtt gcgggccacg tgcagcaccg cctcgagctc
     2401 gaggccgcgc gtgcgtgcct cgccggtctg gagctgattg agcgggttgt tgggatcggg
     2461 cgccttgcgg ttttcctcgc gcagatcgta gatggccgcg ttgatcagcc cgaagcctcc
     2521 ggccggctcg tacttcaggc caagctcgta ttgttcgcca cgcagtggct tgaaggcctt
     2581 gccgtccccg ttcaggccga tggtcggctc gaaggactcg gcgtagctga gataaggtga
     2641 caggccgttg tcggccttgt acatcaacgc gaggcgcttg gtgttggcgt cgtccctctg
     2701 cgtcggattg ccctcggttg cattctccgc acggtcatgg cgcaggccga gcacgacgat
     2761 ccagcgctcg tcccaggtca gttgatcctg aacatagaag ccggtctggc ggagcgtgtt
     2821 ctccggatcg tccacgaagg cgctcgccgc cggtggggtg aagttgccat acaccgggtt
     2881 gtagagattg agcgtgcccg ccgcaccgta tgcccatcgg cggttcgtca ccgcatgctg
     2941 gaagtcgatg ccggccagca aggtttgttt gaggcggccc tgatccagat tggcctgggc
     3001 ttgatgatcc accgtccagt atttgaggtc cggcttcgcc acccagaaga cccgatcgat
     3061 gtcgccgttg ggctgcagtt gcggacggaa gcgcgggtag atcgtctgat agctgacctc
     3121 gctgtccgcg tagcgcaggt tctggcgcaa ggtccacaca tcgttggggc gataggtgag
     3181 cagcgaggtc agggcgcgct gttcggtgtc gtattcgtcg aagccgggct cgctcatgaa
     3241 cacatcgacc gggatgcgcg gcagaccggc cggtgctggc agcaccgtgc ccgcatgggg
     3301 gaggaactgg gtggtcgaac cggtctcgtc cttctgcagg ttcgccagca gggtccattc
     3361 cagttgggca tcggggcgcc aggtgagcga gggcatcagc acgtaacggt cgtcatcgac
     3421 cttgtcgacc tgggttccgc tgtcgcggcc gattgcgacg aggcgataga gcagggtctt
     3481 gtcctcgttg agcggacccg tgatatccgc cgcgagctgt gcgcggtcgt agttgccggc
     3541 ctggagctga atctccccgt ggcgtgtgtc cagcgggcgc ttggtgacca ggttgatgat
     3601 gccgccgacc gagccttggc cgtagagcac cgacgcgggt cccttgagca cttcgatgcg
     3661 ctcgagcgtg aagggatcgg ggcgggcgct ggcgtagtag ccgaaggtct gctgcatgcc
     3721 gtcctgaaac acgaccgggt cggtgccgcg aacggtcgac cagtcgcccc ggctgtccag
     3781 tccgtaggtc tcgcctcgca ccccggcggt gtagcgcaag gcgtcctgga ttgtttgagc
     3841 gccctggtcg cgcatgcggt cggcggtgat gaccgagacc gcctggggcg tctccgagag
     3901 tggggtgtcg gtcttcgtgg cggtcgcgct gcgcttggcg acatagccca gcaccggtcc
     3961 ggtcgccgat tcggtgacgc cgtcggtgac cgttacggcg gggagtgtgg tggtccggct
     4021 ggtgtcttgc gcgattgcgg cgctggtgcc gagaccgaag gcaagtgcaa tcagggaaga
     4081 agcgagtgag caaccggggg cattcgcgac gctcgtgcgg acgaggtgcg cgtggcgaga
     4141 gggaagagtg atcatgatgg gagagtcagc caggtcccgt ggggacgggg tttcagtcag
     4201 cagcctgtgg aacgcacgag gtcatgcgct cgaacaccaa gcgcacgatc ccgagtcgca
     4261 cccgtttggt ccgggttggt cgcttgcacc gatggcggcg caccaacgct ggatcggggt
     4321 ggctggctgg cgaacgacct cgtgcgaggt cttggcagca gggctggctg gctatcccgg
     4381 ttctcaacat aaccaatgag aacggctcga actataaata agagtcattc gcatgtaaac
     4441 gatcatgttc acgagcgagt tcacctcggt gcttgcgatc gagcgcgaac accttcctgt
     4501 gacccagggg cggccaattt acggtgtcgc ctgagattgc tcatccggca tcaggcgtgc
     4561 ttgatgccgg tctccgctcg gggcgttcaa aggtcgcgct gtcctctcgg gcatgaattg
     4621 ctgtacgttt acccacgcgc cgacctcatt ccgctccccg tgcgagaaac tcccatggaa
     4681 cccgctgaca ccgcacaccg cacaacctat gcaattaagc gtaagcactc ccaagtcgac
     4741 gctaccccca cccccgacct cttcgacagc ctcgagcgtt ggctcagcac gcctgaactc
     4801 gcctatgcct catggctcca cccccagccc ctgagggtgt ccaccaaaac ggtctacacc
     4861 gccatgttcg gtcgcttttg ccaatggctc agtgcccaag gccggcgcct cgatcagctc
     4921 gaagccgacg acatccggcg ctttctcgac agcgcaggca gcgcgccgcg ccaacgggcg
     4981 cagagcgggc gtcagcgcca gcagtacgtg cgccagctcg agcgcgtctt cgcgcatctg
     5041 ggcgccttgg gctacggtgg taacaacgtt ggccgcatgg caggcctgga acgggtgggg
     5101 cagggcgacg accagcccgg tcggttcctc acctcggctg aatgtgaggc gctgatcgcg
     5161 cggctgaaaa cgaggttggc ggacctcgag cgcgagagga agggcgccga ggcgtggatc
     5221 gagtatcggg atttggcgct ggtggcggtg atgctggggg cggggttgaa ggtgcgcgat
     5281 gtggtgggcc taacgcttaa ttgcattaat aggggggagg aacgtatcga gctcagccgc
     5341 ccgggctgcg ctcaccgcgc ccgcatcctt gcgtttgcgg tctccccgat ccaggcgtgg
     5401 cttgcgctcc aggaccagat ccacggcaac gccgccgcgt cacccatgcg caaggtgttc
     5461 gaggcggatc gctcggtcgg cttcgggcgc ctgagcaagc aggtcaccct ctccgcctcc
     5521 tcggtacacc ggcgcacgca gaagctgctc gaggaggcgg gtatcaccgg cgagcgtgcg
     5581 tgcgcgcaga cactgcgcaa cacctacgcg ggcttgctca tcgagggggg cgccagcgac
     5641 gagcagttga tcgactatct ggggctgcag gcctcgatca gcgcacaacg cttgcgcgcc
     5701 agcttcgcgc gtgcaaaagc gccgccggcc cagaggcagg gatgatccag tccctcatcg
     5761 ccgaccgccc cgccagcatc gtggccgctg atgctgagcg gccgggcgcg ccgttggaag
     5821 cggcgatgtg acaagcggcg accggggtgg atgacacttt cttgcaccaa caccttgacg
     5881 gaaccgtgcc ccgctcgggc acgtcagccg cgccagatta acgccccgcg cgcggggtca
     5941 agcggcgtcc cagcaaacct cgcaaacgtc agcctggagc agttcaaaga gagttgacag
     6001 aatttgggca gcacgcgagg atgggatcgt attcatttat cttgtgataa ggggatttta
     6061 cgatgacgcc ggagcaggaa aagcagttgt tgcaggcact ctatgaccgc ctgttcgatg
     6121 cgatcactta tgttccccaa ggtggtggcg tcaatccatt caccaagaat gaaacgttta
     6181 ttcactttac aaagaacgag acgctgcagc cctcagcctt cgcggatgcc tggaacccga
     6241 ccaacccgaa cgcagacctc ggcgcgtccg agcttttcgc gacgatggtc gacaaggtcg
     6301 cgcccttgtc gctggaatgg gcgcccaagg accaaccgct tgcgctcgtc tacaagagca
     6361 tcgtcgacgg gatgaacgag gcgaagtctg cacgcccctc gcaggcgtca atcgacgctt
     6421 acaacaaggc gaaggcctat ctgcagaagt cagagaagaa cccgttcacc ggcgagatga
     6481 cggccggcgg gctgagcgac gagtaccaag cctacctgaa gaacctgcag gcgctgaccg
     6541 aggcctattc ggactataca agtcgctaca acacctttgt cgatgaggtc gtcgatgccg
     6601 aggcagcaaa tgacgccaac gccaagaagc gcgcgcagcg cgagtgggtc gccgacgaga
     6661 agggtctgcg gcgcgaaatc tcgaatgccc aggcgaacct gacggccggc aattcgaagt
     6721 gggtccagat ggcgctggac acgatgaaca ccacgattaa cgacgccatc gcacgggcct
     6781 tggagtccgc caaggcacag gtcgcggacg accagttcaa cgctggaacg caaaacaagt
     6841 ggctgctgac ctacgcgaac ccgaccaact ggtacgaccc cagcgcgacg gagaacttca
     6901 gcgagctgac gatctccagc aagaacaagg tggacgacaa aagcacgacc agtcatgcct
     6961 acacgttcgg cgcctccttc aacgccggac tgtggggagt atccgccaaa tcccagggcg
     7021 aattctcgaa tacccagcac cacatgaaag ccgacgagat ctcgatcagg tgcaagatcg
     7081 ccaaggtgag catcgtccgc ccgtggttcg acgagacctt gttccgctcg gataactggt
     7141 tcacgaacct gaggggcgag gacgaaacgc tctacatctc gaacggcaag ctcgacagca
     7201 cgaacgctga cagcatcctg ccgatgtacc cggtcgcctt catcgtggca cgagacatcg
     7261 agatcaccgc caacttcacc aaggaagatc tcgaaatcat caagcaatcg gcgagtgccg
     7321 agaccaaggt cagctacggg ccattctcga tcggtggcag ctacaagtac ggcaacgaga
     7381 aggagcattc tgagttggag gtcagcgacg gcaagctgag ggtgccgggt atgcagatca
     7441 tcggctgggt cagccgggta atgccgccct cacccaagcg gtcgcggaag gtgctggaca
     7501 cctgagccgc ggcgcggcgt cccgcaccat ggccgatctt gcagcatcgt tcgcccgaag
     7561 cctcggggct gcccccgccc gccttggggc ggcccggggc aggaaaccgc ccctcgaccc
     7621 cgagaagggg cggtcggtcg gtgtcctgct aggccagacg ccggggatcg gcggccggga
     7681 tgtcacgacc agagctcccc gcgcgccggg gccggtgctc caactcaacg ccatcccagt
     7741 gtcgatcgac ctggaaaaat ttagcgacgc ctattcgtcg gacaaccccg gcggggatta
     7801 cgaggcggcc tatcgcctcc gcaacctctg caacgcgatc ccgagcttca agcgcaactt
     7861 cgacccaagc ggccactacg ttgaggcaat ctggcgcaac atcgccttcg cggcgaccgg
     7921 gacgacgagc tatgcgcagc acctgctcag cgtggcccag gccgacatcg acggggccga
     7981 actgtcgaac ttgggcggca gccccaagcc atggctgcct gtcgatgcgg cgccctcgaa
     8041 ctggacggag ttgctggccg atgcgccgga gtgcgagttg gacctgggcg gggacggcgg
     8101 ctcgggagac tacgtgctga tcgaccaagc cgagaacttg gcctggaccg gcatgccggg
     8161 ggccgaggcg acgccgattg agggaaagtt gcaacgcatc cggctgcgcg cgctgcgggt
     8221 cgatctgaac cgcagctggc tcgacttgca gttactggcc atctccggct ggaagatcaa
     8281 cgggatgccg agcgggttct tctcctccgg cacgcaggcg gacaattcag ggatattccc
     8341 gctcctgccc accgcgctgg tgatcggcac cgacatcatc atcgagggcg actggagccg
     8401 cgccgatctc aagttgatgg gtcggcacgc cgccgaaggg cgcgcactgg ggatcggccc
     8461 cttccccctc gcggcccccg cgttggcggt cggcgccccg ctgcagatcc agcaggcgca
     8521 tgtgatcggc gtgatcagcg cgctggtccc ctacgcccca caggcgacgg acgtcgatcc
     8581 cggcctcgtg ctggtgaaga acgacggcgg tttcatcgcg cggttcgcgg tcgactggcg
     8641 gctgagcggg cacccccaac gcagcgaaag cggatccttc ccggtggtgg ccgccaagag
     8701 cgtcagcctg cccgccggtg ccaccgacat cgcggtgacc atcgagatca tgacgttccc
     8761 gcccccattc gagacctgga aagtgctgac cgtccgcaac tatgacaccg cgccgcgggt
     8821 gtctttccgc ctgtcgggca caacgatcga cacggtgatt gaggagctgc ccgttttcgg
     8881 gtaggtcctg gggccgcgtc cgagcgtacg cacgtctgaa gctgccggca gaccggcagc
     8941 cacacatctg catttgggga gattggtcga tggcgagacg aacgggaatt ccgggggctg
     9001 aggtgctggc gcgcatggcc tcgatcgtgg ccggggagga gggcgcgcag gtcacactcg
     9061 cggcgcaaac ggacgcgact ttcaccgtaa aagcagcccc ggtgcatccg gtgcaggtac
     9121 tggagaacag ctaccagcgg gcgatccgcg aggcttcgtt ccgcgagccc gatccgggca
     9181 agcggcaggc cgtggagcgg ctggcgctgg cgcgctactt ccccttcgcc gcgggcgagc
     9241 tggagctggt cgccgcgccg gatggcgcgg cggagctggt cgatgcgcgg ttcgaggccg
     9301 gacgcgcggc gatctcgcag gtgcgcgagc tggcgcaggg ccgggtgaac gacggcgccg
     9361 gcggcacggc ggacgatctg gaggccggta aggggctctg ccgcgaggcg ctggggatct
     9421 gtgagacgct cctcggggac tacgatgcga tcggcggcga gatgggctac gacgtgcgcc
     9481 agatcccgat catccgcgag ctgcacgaca tgctgggcac ggggacggcc gagctaacgg
     9541 tcgcgaagga ctgggcagcg atggccgcgg tctgctcggg cacgctacgc cggctgctgc
     9601 tgcgcttcag ccagctcatg gaaggcctcg cttgcgtgca ggttttccac gtgatcgaca
     9661 cgttctatct ctaccggctg ctggtgcgct accgggtcga gggcgaccag gagccgggag
     9721 acgacctgag gatggcggtc agcggcgggc tgaccggact tctgaataac ggcgtgccgg
     9781 tcaaccagga ccgggtctgg ctgtcagact ggctcaaggc caagatgcgc gagatcgacg
     9841 tggcgctgca gcgcatcggc aacgtgcatt tccacctcaa gggcgggcgg gcgcttgaat
     9901 acgtgcgccg gctgcccgaa gaggggcgca acgactggga cacctcggtg ctgatcgacc
     9961 ccgacttatc ggcggacgcc tggtatgcga agttcgccga gatccacaac gacctcgtcc
    10021 cacgcctgca gcgcttcaag cgggagttct tcgtgctggt gcacaagcac gccggcgcga
    10081 tcaacgcctc gctcgatgct tacgttccga aggtgaagcc gaaggaagag gtcgtgcgtg
    10141 atggtgatca cgccgaagag gacgacttcg aggccggggc acgcgactac gacgtcatgg
    10201 acggcgcgct gagcgaccgg gccgagcgcg tgcgcaacga gggcgacgac ggcgcgcggg
    10261 gcgctcccgg cggcatcccg ttccccaagt tcgccgccgc ctgcaaggcc gagctgatcg
    10321 acatcggcat cccgcgccgc gacacggtcg acgccatcgc ccactggcac cacatcggcc
    10381 cggggatcct gcgtaagccg tggaccgagc aaattcccgt gcccgggcac cgctactaca
    10441 tcgacgaata cctgatgatg atccgcgacg ccttcgagac gccggccaag aagatggaga
    10501 agcggatcag ccgactcgcc aacttcctct cgctcgggcc ggaggacaac gaccccgcgg
    10561 aaacgatcga ggtggcggtc gcccatgccc gcgccgaggc cgaggccgcc gggctggcga
    10621 agtcgctcgc agtggtcgac gcccagtccg tgccggtgaa gcggctgatg tacgtgatgt
    10681 tggaggaaat tgcgagctcg cacgagctgc gcagcaggcc cgcactgggt ggcgtgatcg
    10741 acgagatcct tgcgacgcag gcaagcgaca gggcgctgcg cgccggcttg ccggcgacct
    10801 acccggcggc gatcgccgat gcgctgccgg cgctgagtga gccgcacaag gaggtcctga
    10861 gctggatcgc gctgatgcac gcgatctcgc ggcgcttcga gacccatctg gccgacaagg
    10921 cagcatactt cggcttctgc ccgcagcttg ccaccggcgc gccagcagta tcggccgatg
    10981 agaaggcgcg cggtgacgca atcgtggcgc tgctgcgctg gctgcatggc gagtcggcct
    11041 tccgccccga caaagagcac gaggtgcagt tcgcgatcac aggggcgtat gcctgcgcgc
    11101 tgcataccga ttacgcgcgc aaccggctgc ccgcggcgct gaaacaacag ctccagatcg
    11161 ggctcgatcc ggttgaggtg gtggacgtga aactcttctg ccatcccgat ccgcgccgcg
    11221 ccattgaccc cgcgaccgtg ctggaggcgc tagtccggcc gctggtcgag gaatacgcgg
    11281 cgacgaagaa tccgcccttc gcgttccagt ttcaccccga ccgaatcgac gtggtctgga
    11341 aggacgaggt gaagatcagg gacgtgagct accgtccgct ggtcgcgcgg atccgggtgg
    11401 tcaaagaggg ctggcccttg ctggcctatg tctggggcct gccggtactg tcgctggcgg
    11461 atctcatccg cgagtatttc tcgcgggcga gcctgcatca ggacttcctt gccgagaagc
    11521 ggctgaagca gacggcagcg ctgctcacct cgttgctcac ctatttcgag gcgaacgacg
    11581 ccatcgaccc caacgccgtc atcgtcgacc cgacgaacga cgtggacgcg gtgctgaacg
    11641 agatcacccg ggtggaggcg atgacgaagt cgaacgacaa gtcggtcgca ctacagggga
    11701 tcgctcggcg ggcaccctat gacgatagga tcgtggcgcg catcctgcag gcttgcggca
    11761 cgctggcctc ctcgcaagtc gcgcccgttc ttttttcgct actggaccgt gaagacctca
    11821 cgacacaggg gtatgtggcg atcgcttgcg gggctaaggc cgtcacaagc agcagcgaga
    11881 gggaggggct gttgatgcgg tatgccgagg tcgccccgtt ggtcgaccgg gtcttcgccg
    11941 ccctgctcga cgccaccgcg tcgatgaagg cggccgagaa gatcgccgac gtgctgatcg
    12001 tcgtggccaa gcggcgcgac gtgacgaccg agatcaaggc caggctgcgc gagttcgcgc
    12061 aggcgttcgt gaacgtcacc aggcaccgca accgggtaat cgaggcgctc tagcgtcgcc
    12121 accccgctcg gcattgagcg ccacatccgt gcgttgctcg cccacaaacc gcctgacgtc
    12181 gaacgttggt ggtccacggc gaaaacaacg catccacgca cgcataggac caaggcgatt
    12241 cagtaccgac tggacgctgc tcggggcgct cagtctcgca ggattttcga agcgagcatt
    12301 gccgtttcgg tcaatcgggg ccgccccgag tcacagtgca tgccacgtca ctcggaacgt
    12361 tctcgtgatc ggggacccca cacctcgggg cgcaccagac tgctagatcc gcgccggcgt
    12421 ggtcgccctg tagcagactg tgtgctggat gttgcggcac gcgacggagc cgctcgatgc
    12481 ctcgaacgtt ttttcctcac cgaatcctgt cgccgggaaa cgctcatagg atcaggcgac
    12541 tcccggcacc gagactacca agtcaagcga tccgcgccac ccgcttcggc ggcaggggcg
    12601 cgtggctgtt gtgctccttc tcctgttccg gggggcgctc gcgctgcgct tcgatcttgc
    12661 ggatcacccg gacagcctcg gccttcacct gacgttcaac gccttcgcga ccgagaacac
    12721 tccgcgtcgc caagtcattg aagtcggtgt gcgatttcat ccgcgcaagc gcctctaact
    12781 gcgcatcgct gagcagtgcg cctttgagtg cggcgacctg ctgatcggtg agcttcgccc
    12841 cctcggagtc ctgctgtgcg tcctccaacg ccttgatggc ccgcaggtgc gcacggtaga
    12901 tcggcggcgt gatgggcgcc agctcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnctgt ccttggcgaa gcgcttggtc ccgtcctcct ggatgtattg catggtccac
    13081 tgcttgccat cgacgtcgaa cgcggggatg caggtcgtct tcccatccct gtccgtgaag
    13141 acccccgcat ggacccggat gcccttgtca cgcagatagg gcgtcggctc agtcaccgac
    13201 accagttcgg ccatctgccg gcagacgcgc tgcgcgctcg cttcgtgctg gcgcgcctgc
    13261 gcgtcggccc gggcggccag cgtgctcgcc gccgcggccg ccagtctcgc cttctcctgg
    13321 ggatcgagga cgtagccctt cgccttccac gtcatctcga ccccggtgcg gttgttcttg
    13381 atgtaaccgg ccgggtgacc atccaggtgg acgacgtaga accctgcctg ctcgcctttc
    13441 cggtctccct cgacggcgat ccggtgtttg ttgccgtcca tgatggggtg ctcgccggtc
    13501 acgatggccc cgatcgagcg caaggcctcg gcgaactcgt cgcgggggtt catcgccggc
    13561 gcctgctcgg ccggcatctt gtccggcagc cagcggtgca gccgctccat atcggccttc
    13621 ggcccggcat accaggactt cgccgccgca tcccacgcgg cgccggccgc tttcgcggca
    13681 ccgcgctcgc catacgggac cgcgaggtac acgcgccggc gggcgcccgg atcctcgcgg
    13741 gtttgggccg ggaccggggc acgggcgccg ccttgcatcc aggcggcgaa gggggccgca
    13801 tccacgcccg ggggcacata ccaggcttgt tccttgcgat cccagcgcgc ccccagcgcc
    13861 ttggcggcgt ccttctccgt gtagggcacg tgaagataag tccgcgccgc ctctgccggc
    13921 tgagccgacg gtgagtcctg ctgcaccgcg gccttcgccc cgtgcgtctg cgcctcggca
    13981 tccgcgagcg tccggttcat ctccgccgtc ttccgcgcct cactggcggc ggcaatgtcc
    14041 gcctcggtgc tggcaggatc gcgccgcacg cgatcctcgt gcgcgcgggc gaggatcgcc
    14101 tcgcgcgctt gcgcgctgtc ggtcgagttg gcggccacca cggccagccg ataggccagg
    14161 tgctcagcct gctgcgcgtg ccggaaatcc tgcacgaacg catagcgccc atcgtcgcgc
    14221 tggacgtaca cgctccagaa ctgcggcgcc acccccagat cggtcgccgc cacgacgtgg
    14281 gtcgcgccct cgaaatcaag ctcggcgctg gcctgcacct ggacctggcc gttccagtcg
    14341 tgcggcaagg caatgtcgaa ggcttcccgg gccgcggcct cgaaggccgc cctgtcaccg
    14401 gcctgacgcg cctgggccac cttcgcttcc tcgcgcagtt gctcgagcgg cgcgatcacc
    14461 ccctggatgc gagcgtccag cgcatcgatg tcatcgggca gcgcatgccg cgtccagaaa
    14521 gcgttctgcg tgttgagcgg ctgcatctca tcgagcagcg cgcgggccct gccgatctgc
    14581 tcgagcctcg ccgacgccag ggcatggggc agggtgtcgc gcatcagatg ggccagcgcc
    14641 cacgcttcca ccctggccgc gtgctcgcgc ggatcggtcg catcatcggt gtgggtttgc
    14701 atgctcacct cctcggtcgg gatcgcctgc tgcacggaat cgcgcccggc gacgatgtgc
    14761 cgggcgtcag ccaccgcctc gcgccatgcg tcggccgcgc tcttgaacgc atcggccgcc
    14821 gcctgcacat ccgggtcgtc atggctcggg gcatagcgcc gatcgccggc cgccgtttca
    14881 ccataggcgc gcacgagcgc ggccccgaag gccgcgtcgg cggctttcat ggccgcgtac
    14941 tgcgcggcgc tcagctcagc cacggtcggc gggggcggga gcaggggagc ctgatccggt
    15001 tcctgttgcg gcccgtgttc ctggacctgc cgctgctcga aggccatcac atagtccttg
    15061 atcttctccg cctcggccgc cgcccgaaac acttccagcg gatcggcctg cagggccttg
    15121 atccaggacg ccacataggc gacgtgctgg cccggatcgt ggccgatccc caactcgtcg
    15181 cccagaatca tcgaggcgat ctcggcccgc agttcctccc gggcatagcc ctcgctgccg
    15241 cgcgggtggg cgaggtcgcg atcgagccgc gtggcgtgtc cggtccagtg tccgagctcg
    15301 tgcagggcgg tcgcgtagta gttcgatgcg gtctcgaact gacccttgtc ggggaggtgg
    15361 atgctgtcgg tggcgagccg atagaaggcc tggtcgatct cgccgtgccg aatcaccgcc
    15421 cccgaggcct gcaggatgtg ctcggctcgc tcgagggcat cccacgccgg gggctgtcgt
    15481 tcgagcggca ccagaccgtc gatctgctcc gcattgaaca ccgtcgcaaa gaacacccgc
    15541 ggccgctcga gcacgacggt cacctgggcc ggccggccct cgccgtcgag cacaggccga
    15601 ccgtgctcat cccgcagcgc ctgctcttcg ctgaacttcc agtattgaac cgcggtgccg
    15661 tgctcgccct tgcggacctg cgccccggcc gcggccgcct gcttgtaggt catccagcgc
    15721 ggatcggtgc gcccctggct catcagatgg atcgcgttga tgcccttgta gcgcttgccg
    15781 gtcgtgggat ttaccggcag catgctgccg ggctcgccag gcacccaggg tcgctgccag
    15841 ggcgccgttc ccgccttcaa ctgctcgatc aggcgctcgg cgacggtctc atggaagggt
    15901 tttctgcgct cggccatgat ctcaaccctc ctgccgagga gtgggagcgg tcgcgcccgt
    15961 cccatcgaac gggctctcga gcgcgtccgc ttcgcttaag gcgtcttcga cgaaggcgcc
    16021 cgcacgctcg gcttcaatcg gatcgaactc ggcctggtag ccgggcgtga gcgcatcggt
    16081 cagcttctcg cggaccgtgt ccggggccgg tcgacgcgca tcgagcgcgg tgtcgggctg
    16141 ggggatcggc ttggtcatgg gcaggactcc tgtgcgaggg ggagggagaa agggggccgg
    16201 ggcgctcgcc ggtgccggcc gttcagcgat cgatcacggg gggtgcggtg cgtgctcacc
    16261 aggtcagtag cggcaccacg acatttagaa tctgcccgcg cgagacgggg ccgaagtagc
    16321 gggcatcgaa cgaggtgggg ctcgcgtccg ccatcaggag cacctcgccg gcgccgagca
    16381 cggtcgcgtc ccgccggaat ccaggcagcg cccgcccgcc agcatcgacc gcgcgcggcg
    16441 cactcagggc caggagcgcg ccattgacgc gcacgcccgc ctcggacacg gccacggcat
    16501 cgcccccggt ggccgcgatc cgcttgatga gatacccgta accgccagcg caaaatcccg
    16561 cgccgatata accccgcgcg cgcgcctcat cgaagaccgg gccctgcggc gggcagacca
    16621 ggacataggc gcctttctcc gccgccgcgt cgctcaccca gtacaggccc agcgggacgc
    16681 tgcgggtcgt gttgatgcgc gcaccggccg cctgggcgag cgcggccacg gccatggcgg
    16741 cagcgccggc cagtgccacc ccggccgcga gtcgcgcgaa gcgccccgac atcgccgtgc
    16801 cccgcgtcag cggccgatcc gcggcagacc gatcccgggc ggtcccatgc ggtggtgccg
    16861 gcgcgggccc ttcatgaccc cgctcgccgg gcctgcccga atcggtgtgc ccccgaccgc
    16921 gcgcgcgaac gcgtacgcca ggcgcccccg ggaagcgccg cccgctcatc acgcgccccg
    16981 ctcgccggcg tcggcgaacg cgatcaggcg gtcgctcacc gtgggcgccg gcaccgccgc
    17041 ccgcgcccga aagaccggat ccttgaagta gagcggctgc ttgccataga tcgccggata
    17101 gcccgccaca tacacgacca tgtcgcccgc cgcttcgatg tggccctcgg cgtccttgcg
    17161 cggcccgggc atgcgcaggc actcgtcggg ggtcagcaag ggccgttgca cttcctggaa
    17221 ggtgcgcgag acctgcccga gcatcgcggc cgtgcgccga ccgctggtgg tgatctgctc
    17281 cttgaccacg gtggtctgcc cggtgagccg cgacaggtgc tcggccgtct ccacgcggtt
    17341 gggcggatag gcgttctgca catggcagtt ggaggtgatg gtctcgtccg gaccgtagcc
    17401 ggtctcgcga ctcttgagct ggttgaggtc ctggcagatc aggtagcact tgatgccata
    17461 gcccgcgacg aagcccagcg actcctgcag gatctgcagc cgaccaaggc tcggaaactc
    17521 atcgagcatc agcagcagcc ggtgcttgta gtgggccacc ggccggccgt gctcgaagtc
    17581 catccggtcg gcgagcccgc gcaccaccat gttcaccatg acccggacca aaggccgcag
    17641 gcggtccttg tcgttgggct gggtgacgat gtagagcgtc actggatcgt ccgcgttcat
    17701 cagatcgcgg atgcggaact cggagcggct gacgttgcgc gccacgacgg gatcgcggta
    17761 gagcgcgaga taggacttgg cggttgagag caccgaaccg gcttcctctt cgggccggtc
    17821 catcatgtcg cgcgccgcgg agccgacggc cggatggttc tggccgtcga ggtgcccata
    17881 ggtcgccatc tccatccaca gttcagcgat gtcgcggttc ggattggcca gcatcgcatc
    17941 gaccgccggc agcgtcgccg cgctgccctc gtcctgcgcc ttgtagagcg cgtgcaggat
    18001 cacgccgacc agcagtgcga acgcggtctt ctgccagtgc gagtccagac ccttgccgtt
    18061 cggatcgacg atcagcgtca cgaggttctg cacgtcgccg accgcgtatt cggtgtccag
    18121 ccggatctcg gccagcgggt tccagcacac gctgccgtcg gccgccgccg gctcgaagcg
    18181 cagcaccgtg ttccgggcgt gcgtcttgcg ccacccggcc gtgatcgccc acaactcgcc
    18241 cttcaggtcg gtgaccacgg cgctcgcccc ccaggacagc agggttggca cgatgagccc
    18301 cacgcccttg cccgaacggg taggcgcata ggtaaggacg tgctccgggc cgttgtggcg
    18361 caggtaatgg aaggtaccgt cctcgtcctc ccagccgccc acgtagacgc ccgcagcgcg
    18421 ggccgccgtc ttgcccgtca ccacctccag ccacgtgcgc ggccggggca gcaggccggc
    18481 tgcctcgata tctttcttct cggcccagcg cgccgaacca tgcaggtagg cgttcgcacg
    18541 cgacgaattc gacgtgacga ctttcgcgac cgccacgccc agcaggccta ccgtggccac
    18601 cagcaggccg acactggcgg ccgtcatgac ctcatctgga tagcgggcat accacgtcga
    18661 cgaccactcg aggatcgacc acggcgcata gacgtggtgc acgtggcgcc ccagttgggc
    18721 ctggtagtcg aaggtgtggg cgaagaactg ggtcgccgac tgcagcccgc cggcgagcga
    18781 cagcgccccc agcgcggaca gcacacggct gccactcgct ttgcgggcgc gcacctgcgg
    18841 gccgaccgcg ttgttcatgt tgattttcat cgactccgtc ctttcgacat cgccatcgtc
    18901 ccccgcgggg tcaccgtgag cgcctcgccg agcgccacgc gcgacaggcg ccgcgcagtg
    18961 gccgcatcga cgggcaacac ctggatctcg tctgcctcgc cgtgccgtag cagcgccagg
    19021 gcctcctcgt cgatgcgccg aagacccgcg aagatcatga caccggcaca tccattatat
    19081 agcgcatgtt tcggtatacc gataccttgc acgcgctttg cattgcgctc ggcgatgtag
    19141 gtgtcggccg ccgcccgctg cgccggggtc agtcgcgggg cgtctcgtcc tcctgcccca
    19201 ggaaggtccc gtcgcagtgc gtgatctggt tgggggcctg gctgctccag gtgacgagga
    19261 acatcacccg gcagtagcac ttcagctccg tcggcgaagc gaaccacacc gagttcggac
    19321 agtgctcgca gacggtgccg gcagtggggc ggcgggacgc gtccagggcg tccagggtcg
    19381 ggctggcgga gggcaggggc gtctcctccg ccgcgctcat cgcggccgcc tcggcggggc
    19441 gcgccgacgg tggcgccgtc tccagcaccg cgagggcggc gtcgatcagc gcgtcctcgc
    19501 tcagcgccgt ctcgtcggac gtgtaaggcg tgttcatggc caggctcctt gctcgagagt
    19561 tgttgccgcc gcagttccag ggcggggtcg gtgaaggtga ggggaagctg cagatccacc
    19621 gccacccgga tgacctgcgc cttgaaggcg ggcgtgccgc tcacggtgag gcgcgggcca
    19681 aagcgtgtca gcgccaggcg cagcgcgatc tggacgccct cgcgcgtagc ctcgtccgac
    19741 acctggaggc ggtcgccatc gtcgcgcacc gccgaggcgc cggcgcgata gaggatcgtg
    19801 cccttcttgg tgatggtgtc gcgcacgagc ggctcgttgc ggaggggatc cggaacggaa
    19861 ctcgggccgg accgacgccc acccccgccc gtgacggtgt tgccctgcag gccccgggcg
    19921 acctcgcgcg cgcgcagcgc cgcgagcgct tcggcgttgc cgtgcagcgc ctcgtgcttg
    19981 agccagtcgg cccacgcccg ccgctgaaaa cccccataca gcgcctcgcg cgcgtctgca
    20041 tacgcggcgt ggatcgcggc gagttgcgcg cgcaaggccc gactcgcctg ggcgtagagc
    20101 agccgcttgt cgatgccctt gccgtctgcc accttgatcg tggcccgcca caagcggttg
    20161 gcgcgccggg cggcctcgat cgcgcgtgcc ttgcgctgcc gggcgctcgc cagcgcatcg
    20221 gtgcgttcgg ctgagcggcg ctgctgctcg gcctggtagc gggcatagag tgcggcggtg
    20281 tcgactcgca gcggcagtgg tgccttgtcg tagtgccgcc ggnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nncggacgcg gtccgtccgg cggtgcctcg aacggcccca
    20461 gccgcgcctc gaggctcggc cgcgagagtt cgcggccgag cgtgctcgcc ttgacccgcg
    20521 tcccgtccga cgcttcgacc accaaaccgt tgccgcgcgc gcgcagcacc aggccattct
    20581 cgtgcagcac ctgatgcagc gccgcccagg agggcgccgc gcgcagcgcc tcgagacagt
    20641 gccggcgcac ccagccgagg aggctctcga taccggcatg gcactccatg tcggccgcac
    20701 gcccttcggc caccgaccgt cgcgggaggt ggttgtctgg ctgcaggccg tactcacgct
    20761 cgagcgccgc gcacaacgcg gccagcgtgc ggtacgactg gaacggctcg tgcatcgtgt
    20821 gccgttccgg atggatcttg ttgatggcga tgtgcaggtg caggttgtcg gtgtcgcgat
    20881 gcaccgcgct cacgcgctgg tggtcgccgt aacccaggcc cgcgcacagg cgcgcctcga
    20941 tcgccgtcaa cgtcgcagcg cccagtgcct ccccagcccg aaagctcacc agcaagtgaa
    21001 acgtcttgtc ccccccggcc cgggtgttct gccgctgcgt ggccagcact tcgccaatca
    21061 ccgcctgcag gctcgccgcc gcacagttcg tcacggtgat ctggccgagc cggtgcgccg
    21121 tgtcctgctc gtcggtgatg tacgccgcca gtccggcgaa atcgctgcag gcagccgcgc
    21181 gcatcggcac atgcttggcg atcatgggcg gcccctaggc gttttagcgg ctaaaaggct
    21241 catcgctgcg cccgcggcat gacgaccttc atcatcactt ggcccatctc atcctgcgtg
    21301 gcctcgatgc gcgcgagcac cgcgcgcagc gtcgcctcgc cgaactgcgc cgtgcgctca
    21361 tcgttggtga gccacagctt gagcagtccg cccagacgac ccaggtcgcc gttgatccgc
    21421 gcgagctcgc gcacctgctc gtaatcgacg accccgtcaa tgcgatagcc ctgaccgacc
    21481 tcgcgcagaa accgggcgac gctcatgccg gcgcgctgcg cctgcgcttc gatgagcgcc
    21541 ttctcgtcgg caaacaccgg cacgcgcagg tgctgcgtgc ggcgtcgggc actgggcgtc
    21601 gggtcgtgtt ccatcgtcac acctcgcagg ggatgccggc ggcagccggc ccggcctcgc
    21661 agagcagggt ctccgttgag cgcccgcagg gcgcgaataa ggcgcaagcg aagggggatg
    21721 accggcttgc cggttagcca acttcgccca tcctgcccgc ccttcagcct tcatgacgcc
    21781 aggaaaagcg ctcatcgccc aagccgattt atgctcactt ccacgcaaag cataaacgga
    21841 tatagcgaat tttaccaaca tcgcccataa tcccggtgtc gtagcgcgaa cgttgcgccg
    21901 aaattgccgc ttgttgcgcg caataggaat ttcttgcggt gctgttgcac accgcatggc
    21961 gcgggcatcg gcacgatgcg cacagggagg acgatcagga tggcaagccg ttacgccgac
    22021 gaactggccg agtgggtgaa cgcgcgcccg gccccgaggc cgcgccagga ccgcagcctc
    22081 gtggcctttc tggccgtgaa agaggacgtg cgcgccgcac tcgaggccgg ctacgcgatg
    22141 aagacgatct gggcccacct gcaggccacc ggccgcatcg agtcccgtta cgagaccttc
    22201 acgctgcacg tgaagcggca tctgcgcaca ccgccgcccg cgcagccccc aaccgccgcg
    22261 caggcgcaac gccccccagc gggaccggcc gccacgagcc cgcccgcgcc ccgcgaaccc
    22321 cgcaagacgc tgccgccctc ggtgggcggc ttcaccttca acgccacacc gaagacggag
    22381 gacttgttct gatggccaag atccacatgg ttttgcaagg gaaggggggc gtgggcaaat
    22441 cgatgatcgc ggccgtcctc gcccagtaca aggcgggaaa gggcaacacg ccaatctgca
    22501 tcgacaccga cccggtcaat gccaccttcg ccggctaccg cgcactcgac gtgcgccgcc
    22561 tcaagctgct cgacggcgac gagatcaaca cccgccactt cgatgcgctg atcgagcaga
    22621 tcgccaccgc cgcggcggac gtcatcatcg acaacggcgc gagctcgttc gtgccgctgt
    22681 cccactacct catcagcaac caggtgccgg cgttgctcca ggagttgggg cacgagttga
    22741 tcatgcacac cgtgatcacc ggtggccagg cgctacagga cacggtaagc ggcttcgccc
    22801 agctcgccgg ccagttcccc gcccagtgcc agttcgtaac ctggctcaac ccctactggg
    22861 gcgcgatcga gcacgaaggc aagggcttcg agcagatgaa ggcgtacgtg gcacacaagg
    22921 cccgcgtgtc cgccatcatc caggtcccgc cgctcaagga cgagacgtac gggcgcgact
    22981 tcgccgacat gctccaggcg cgcctgacct tcgacgaggc gctggccctg agctcgctca
    23041 cgatcatgac gcgccagcgc ctcaagatcg tgaagacgca gctcttcgcc cagctcgata
    23101 atgccgcggt gctgtgatgt ccgaccggat cgaggcgctg gtcaaggaga tcgcggccaa
    23161 gcacggcatc gccgtcggcc gcgacgaccc gatcatgatc ctgcagacga tcaacgaacg
    23221 cctgatacag gacagcgcgg ccgcccagca ggacgcgctc gaccgcttca aggaagagct
    23281 ggaatcgatc gcgcaccgct ggggcgagga cgcccgggcg aaggccgagc ggaccctgac
    23341 cacggcactc gcggccggcc gggcggcgat gtcgaaggac atgcaggccg gcgccaaagc
    23401 ggcggcgctg tccgcgcggc aggaaatcga tgcggcgctc gcccagctcg ccgatccgat
    23461 ccgggacggc cggcgcatcg cgattctgaa cctgatcgcg gccggcatgg ccgtctttgc
    23521 ggcggccctg gccctgctgt cgacgctatg aggccggcgc acagaagctg cggcgagggg
    23581 aggcgagacg aggcgggggc gagacgaggg aggcggggcg cgcgggggtc gaccgggtga
    23641 ccatcttccg cggcatccgg cgctacggcc
//
LOCUS       DAOQBS010000039        23523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1403_length_23523_cov_13.753622, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000039 DAOQBS010000000
VERSION     DAOQBS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 23523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23523
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1403_length_23523_cov_13.753622"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PKN25_06410"
     CDS             <1..450
                     /locus_tag="PKN25_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNV90564.1"
                     /translation="FLGAGVWGFLHTLAPVNYFTHGSQITAAHGHMAFYGAYVMVVIT
                     MISYAMPILRGRAANSTKAQVLEMWAFWLMTIAMVFITLFLTAAGILQVWLQRVSDTP
                     MSFMATQDQLMLFYWMREWAGVMFFIGLLAYLASFFVKGDAKGVVHG"
     gene            716..1762
                     /locus_tag="PKN25_06415"
     CDS             716..1762
                     /locus_tag="PKN25_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNV90565.1"
                     /translation="MHPSGVERPFRIAINGYGRIGRCYLRALHEAGLAERYRIVAINE
                     PADLASIAYLTRFDSTHGRFPGVVDRSERELIVDGHAIHVSHASTPEEVDWTALGLDL
                     VVECSGQYGGRRDLQRFIAAGCPRLLLSHPGNGAEDVDVTVVFGINEDELRGPGRLVS
                     NASCTTNAVVPVLDCLHREIGIEQALLTTLHSAMNDQPLIDGYHHADLRRTRSAMQSI
                     IPVSTGLARGVERLLPALAGRVQAKAIRVPTHNVSAIDMVLSFGREVSAEAVNRLLRE
                     TAAGPQRGLLACSDEAHASIDFNHDPHSAIIDCSQTRAVGAKMLNLVVWFDNEWGFAS
                     RMVDVTRRWLAAAG"
     gene            1829..2443
                     /locus_tag="PKN25_06420"
     CDS             1829..2443
                     /locus_tag="PKN25_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNV90566.1"
                     /translation="MNTVTTPPSSLPAGGPAQAHGPRLAGDLAIWFFILAELLAFGVF
                     FAAYAFARAKNIELFAAEQAALNRNAGALNTVLLLTASYFVVRAVQAAEAQASRQCAN
                     WLGGAILTGLGFIVVKLSEYAAAFEHNISLSSSTFHMFYLSLTFFHFMHVILGLVILI
                     AMWNGARQGRYRPGEMNGLETGAAYWHMVDLVWLILFPLVYVIH"
     gene            2454..2735
                     /locus_tag="PKN25_06425"
     CDS             2454..2735
                     /locus_tag="PKN25_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNV90567.1"
                     /translation="MSARMHGTPRGSTILFATLILATVATWGMGSLGVTGTWGVAILA
                     AISFWKGAVVILDFMALRHAPLLWRAITMGWIIVVWALIAIAYIKGLAQ"
     gene            2732..3532
                     /locus_tag="PKN25_06430"
     CDS             2732..3532
                     /locus_tag="PKN25_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNV90568.1"
                     /translation="MKPDTPNLDIPFYQPAGDEVDIFQHAWKNRLPLLIKGPTGCGKT
                     RFVSHMAARLGLPLYTVACHDDLTASDLVGRHLIGDGKTMWCDGPLTRAVREGGICYL
                     DEVVEARKDTTVVLHPLADDRRVLPIDRTGELLEAPPSFMLVVSYNPGYQNLLKGMKP
                     STRQRFVSLRFDFPRPEQEEAVLIGETACAPDLAKRLVAIANALRALKDRDLEEAAST
                     RLLVYTALLVKDGMDPVAASRVGIVESLTDDADVAEGLMEVVAATFGR"
     gene            3549..3956
                     /locus_tag="PKN25_06435"
     CDS             3549..3956
                     /locus_tag="PKN25_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90569.1"
                     /translation="MEIKRADPLRHVMPGPRLAVVAEALFLINLMLLPGLAFLALMVL
                     WYLHRGHPDPLVRNHLRQTGWTCIWGGLLLVVISVAIFLLGGFDNPWTWVIGVLYFTF
                     VHSSLILLGVMGLSRAINGRSWRFPVFGPREPS"
     gene            3974..4993
                     /locus_tag="PKN25_06440"
     CDS             3974..4993
                     /locus_tag="PKN25_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV90570.1"
                     /translation="MNTGAPARKPQRVIPIATAPAGDAAAAAAMQRRRRAFQAGFFAL
                     FVLAPVFDLFRYDLDADHAWLLGMEWRLGLDPFLAGQASALEAAGSIILNLFVPLLAL
                     AALVIGAAWKWGRLYCGWLCPHFSVVESINRLMARASGKASVWDRHLLPAWKPDGRPA
                     RVDARWWGVVVPAAVGFAFLWAVVLLTYLLPPAQVYGNLFAGELTRNQAIFLTAATVV
                     LSLEFLFARHLFCRFVCAVGLFQSLAWMGNRDALVVGFERERASDCSNCLPDRQSACD
                     AACPMRLRPRNIKRHMFTCTQCRQCVDACATTQHANPQGPLLSWVAGEAARQNEAGFR
                     ALKDR"
     gene            4998..6911
                     /locus_tag="PKN25_06445"
     CDS             4998..6911
                     /locus_tag="PKN25_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HNV90571.1"
                     /translation="MEETVGLLWHRLITRAAGGHFPKAAVRLRDIEKTAGVFFRALGG
                     DPGLRLAAATTDEHGARRSLLQRIAGADERVARARMDVSTLRLPPELDVLPERGLNRD
                     LYLWLAAVAAAPPAEDGDGHPALGPVPDEPIDEAATRELRENQAATLRALARWPGIQA
                     RYRRLVDAVIAQRPRLDKLPPAEAEREKLIRLALHAPGSVAALPRLPAKARATQPVLL
                     WLSEFHASAAGGSGGAGAGGELGEAGASRPGKDSSRQAHRVERQEKMPEKHGMIIPFR
                     AESLLSVAEFIKVQRSTDDEPDDNAADAAANLDHLSITRDGERVASKVRFDLDLPSAA
                     EDDVVLGDGIPLPEWDYRKNLLLEDHVRLAELTPSIHDPRAAPCALPEHLRRTARRLH
                     RQFAALTPGRRWLKAQVDGTELDLDAVVRAATDRATGHHPSDQLYLSLEKRERDLACL
                     ALADLSLSTDSWVSSEARVIDVIRDSLLLFGEALLATGDSFALCGFSSVKRSNVRFHR
                     LKDFDQRFDDRARGRIMAIKPGYYTRLGAAIRHATTILDRQRAARRILLILSDGKPND
                     LDLYDGRYGIEDTRVAVVEARNRGVVPFCVTIDREGASYLPHLFGPAGYAVIRQPDEL
                     PARLPMFYAQLTR"
     gene            6916..7677
                     /locus_tag="PKN25_06450"
     CDS             6916..7677
                     /locus_tag="PKN25_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNV90572.1"
                     /translation="MPRNATPVGERPVRIDSSPASPVTEIDRLCRLLARAPLFNGLAC
                     EDIVRFARGVREVRVARGEILFHRGDPCHGFHLILDGQIKLAFTSAEGNEKVVDILYA
                     GRSFGEAVMFMEKPYVVMAQALTDATLLHIGKQIFFEEMSGDPLFCRKIIAGLAQRLH
                     LLMADVESYSLRSGRDRVVGYLLREEERDGDGVAEGSVSLRLPTSKGTIASRLNLTQE
                     HFSRILSELTSAGLIQVEGRTIHIPDIGRLRRSLG"
     gene            complement(7842..9029)
                     /locus_tag="PKN25_06455"
     CDS             complement(7842..9029)
                     /locus_tag="PKN25_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNV90573.1"
                     /translation="MALIRLDEPTTKRIPPDTFSLWALAFRPFYLLAALFAAIAVPVW
                     AVAYSGAIDLPMPGIWWHSHEMIFGFAVAVIIGFLFTAGRNWTGLDTPTGKPLMVLAA
                     VWLAGRLAMAFGSGAWVAIVDLAFLPVAAGMLLKVLIKAKSKRNYFVGALPAMLALAN
                     LFFHLAVLGVIEADPLTAMHLALGLVVVLETIVGGRVIPMFIFNAVRGVKQWRNVKVD
                     WAAAIATGIALLLWALGAGGWAGVVSLIAAVLQAVRLGGWNPWATRGHPILWVLQLAY
                     LWIPLGLALLALTQFGVLPRSAGVHALAIGATGGLIIGMITRTALGHTGRMLIAGRIE
                     TAAYALVLIAALVRVLTVGFFPAAQFGGVHAAATLWSIAFLLYLWRYAPYLWYARVDG
                     KEG"
     gene            complement(9109..10752)
                     /locus_tag="PKN25_06460"
     CDS             complement(9109..10752)
                     /locus_tag="PKN25_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNV90574.1"
                     /translation="MKIRASTLTGLFALAGALVVLPAAASPDKAVARAASGAAASPAA
                     IDVPALYAQHCAACHAPNRLGAMGPALLPDNLGRLRKSEAAKVIAEGRPATQMAGFAA
                     QLGKDEIAALTEWIYTPVVPEPRWTDEDIRASRVVHVDTATLSDKPVFDADPLNLFLV
                     VEAGDHHVSVLDGDRLEPIHRFQSRFALHGGPKFAANGRYVFFASRDGWITKFDLWNL
                     KVVAEVRAGINTRNVAASPDGTHVAVANYLPNTLVLLDGELNLVKTIPAMDRDGKKSS
                     RVSAVYDATPRQSFIAALKDVGEVWEISYTKAVEDIPVSYIHDYTQREGSFIPGYLNP
                     RRTILAEVLDDFFFTQDYSELMGASREGVGQVVNLDVRKKIANLPLDGMPHLGSGITW
                     EWQDPAAGPGARPRTVMASTNLKAGEVTVIDMKTWEVVKRIPTRGPGFFLRSHSSSRY
                     AFVDSMMSAEAKHILQVIDKQTLEVVHEIPGEPGKTLAHVEFTRDGRHALASLWEDEG
                     AVIVYDARTLEEVKRLPMRKPVGKYNVWNKITREEGTSH"
     gene            complement(10749..11957)
                     /gene="nirJ"
                     /locus_tag="PKN25_06465"
     CDS             complement(10749..11957)
                     /gene="nirJ"
                     /locus_tag="PKN25_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HNV90575.1"
                     /translation="MFRISQFIRELDHPTPAGPRRNPPGPVVIWNLIRRCNLTCEHCY
                     SISADKDFPGELNTQEVFKVMDDLKDARVPVLILSGGEPLLRPDIFDIARRAKGMGFY
                     VGLSSNGTLIDESNIDAIDEIGFDYVGVSLDGIGATHDHFRRKQGAFEASMHGIRLCR
                     ERGIKVGVRYTMTEGNAHDLPALLKLVEDEAIDKFYFSHLNYAGRGNKNRAGDARHRT
                     TREAMELLFETCLDLHRRGIDKDFVTGNNDADGPFLLHWVQRRFPDKAAHIEARLRQW
                     GGNASGVNVANIDNLGIVHPDTMWWHVPLGNVRQRAFGEIWNDLSNPLLAGLKQHPRK
                     LEGRCGACKYLEICNGSSRVRAEQVTGNPWAEDPACYLYDEEIGVNPADYGAERVVTT
                     PWTRIDKVSA"
     gene            complement(12071..12571)
                     /locus_tag="PKN25_06470"
     CDS             complement(12071..12571)
                     /locus_tag="PKN25_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNV90576.1"
                     /translation="MDEAALAEFDRRLVLATQGGLPLVPRPYAALAETLGVAEGRVRE
                     RLAAMLADGRIRRIGAVPNHYAIGYTANGMSVWDIDAAVIDAVGERVGALDFVTHCYR
                     RPRHLPDWPYNLFAMVHAASHEAALARVEEIAALIAAEFPGACRNRDVLFSSGILKKT
                     GLRIGA"
     gene            complement(12640..13143)
                     /locus_tag="PKN25_06475"
     CDS             complement(12640..13143)
                     /locus_tag="PKN25_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HNV90577.1"
                     /translation="MSEAQPAASVGRRGVAAKRAPDDTDRRLINAIQGEFPLSERPFA
                     EVGVLLGLAEDEVIARLQSLLDDRVLTRFGPMYQIERMGGAFCLAAIAVPEAQWTSAV
                     EAVNAFPEVAHNYRREHALNMWFVLATETPEGIADCARRIEAATGLVVHLFPKEREYF
                     VEMRLEA"
     gene            complement(13136..14185)
                     /locus_tag="PKN25_06480"
     CDS             complement(13136..14185)
                     /locus_tag="PKN25_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNV90578.1"
                     /translation="MPRQAILPAALDATGFRLLNEWQRDFPLEPRPFARIAQAVGAAE
                     EDVIAAYRDLSAQGLVSRVGAVFAPRRLGASALAALAAPPERLEEVAARVTREPAINH
                     NYQREHRYNLWFVVTAASRAQLEEVVAGIERDTGCAVIVLPLEEEFHIDLGFDLKAGG
                     EGRRPHADARSDEPIARGDADGGCALPALERKLIGALQGGLVLDARPFATLAARVGLT
                     EGEVIACIARWVDEGLIRRFGVVVRHHELGLEANAMCVWDVPDAEVSGLGRRLAQEAA
                     VTLCYRRRRVLPGWPYNLFCMIHGSAREEVLAARADLAARLGLDRHPHAVLFSCRRFK
                     QTGARYLDAKDLAHV"
     gene            complement(14185..15393)
                     /locus_tag="PKN25_06485"
     CDS             complement(14185..15393)
                     /locus_tag="PKN25_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNV90579.1"
                     /translation="MSCREVRLAPPALWGVVLSAMVVLLSACSSVPPVAVRGTGDLGV
                     VIERADGKVKVIETTGRSVLATVDGLGDLSHASVVFSRDGRYAFVFGRDGGLTKVDLL
                     ARRIVGRKVQAGNAIGGSISHDGSLVVVQNYEPGGIKAFDADTLELVADVPATTDDGT
                     RSKVVGLADLSGKRFIYSLFEAGEIRITDFSDPKNPLTRRFAGGKQPYDALVTPDGRY
                     YIAGLFGEDGLALIDLWNLDKGSRKILSGYGRGEQPLPVFKMPHLRGWSIAGNRAYLP
                     AIGRHEVLVVDTATWQEVDRIQVKSQPVFAMARPDGREIWVNFAFPDNGWVQVIDTVS
                     GKVTDTLQPGRGILHMEFLSKGHEIWLSARDDNKVVIYDTASKQPIGGFESASPSGIF
                     FTTRAARTGF"
     gene            complement(15498..15881)
                     /locus_tag="PKN25_06490"
     CDS             complement(15498..15881)
                     /locus_tag="PKN25_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNV90580.1"
                     /translation="MKIPPLLAHAVVFLGAALVSAYAAASGHVPVEPAPARARELVHI
                     VRQDCGSCHGLTFKGGLGPALTAEALADKPAEGLVATIVGGRPGTPMPPFQSILSEAE
                     AEWIVYWLMRGFPADAGLPQAPARN"
     gene            complement(16064..16393)
                     /locus_tag="PKN25_06495"
     CDS             complement(16064..16393)
                     /locus_tag="PKN25_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNV90581.1"
                     /translation="MNARLVPASLFAVAIGLSAAVPAQAAEHQVVVEKYAFSPMELRI
                     KRGDTVTWVNNEKRVSHSVLLIGRNEESERFFPGEKWSRAFPEAGRFEYRCGPHPEML
                     GVVIVAE"
     gene            complement(16534..17412)
                     /locus_tag="PKN25_06500"
     CDS             complement(16534..17412)
                     /locus_tag="PKN25_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNV90582.1"
                     /translation="MTMKKNLIAGAVGALLAIGTGSALAAAPDWSKVQGKDITAFYPG
                     VSPLEWIQKGTEHGGARALKKGETCADCHSDEAGDMGKKMASGQKIEPSPIPGKAAFI
                     PVKVQAAHDGENLYIRFSWKQPAASGAAKMDDKNPVKIAFMLESGGKVELADQAGCWA
                     TCHTDSRTMPGADDAKTKYVKGGSVAEGKFYDLYQWRSGENKGYNGYVADKRVMEGGN
                     ALVSAEGKKDGDNWSVVFTRKLAAGDGDVKLESGKAYNFGFAIHDDNAAGRFHHVSLG
                     YKLGLDTDGHIKAAKQ"
     gene            complement(17455..18066)
                     /locus_tag="PKN25_06505"
     CDS             complement(17455..18066)
                     /locus_tag="PKN25_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNV90583.1"
                     /translation="MSDQNSDNSKQENPGGLWARLRRPSGKSLGGLLAVGFMVGVLFW
                     GGFNTALEATNTEKFCISCHEMYDNVYMEYKETIHYNNRTGVRATCPDCHVPKDWTHK
                     MIRKVQASKELWGKMVGTIDTREKFEAKRLQLARNEWKRMKAADSRECRNCHSFESMN
                     PEAQKQRARKQHEMAKEDNMTCIDCHKGIAHHKPEGMTDADEE"
     gene            complement(18135..19817)
                     /locus_tag="PKN25_06510"
     CDS             complement(18135..19817)
                     /locus_tag="PKN25_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNV90584.1"
                     /translation="MQKKGLIGSAFAMGVLSLAMSSAWAQEAPKLTAEEMEKGKQIYF
                     ERCAGCHGVLRKGATGKNLEPHWTKTAPDGTKLEGGTLKLGTERLEKIISYGTEGGMV
                     NYDDILTKEEINLMARYIQNTPPIPPEFSLKDMKDSWKLLVPVKDRPTKQMNKVNLKN
                     VFAITLRDAGKLALVDGDTHKIWKILDTGYAVHISRLSASGRYVYTVGRDGLTTIIDM
                     FYEEPTTVATVRLGSDARSVDTSKFKGYEDKYLIGGTYWPPQYSIMDGETLEPMKIVS
                     TRGNTVDGDYHPEPRVASIVASMTKPEWVVNVKETGQILLVDYSDIKNLKSIAIESAK
                     FLHDGGWDMSKRYFMVAANASNKVAAVDTKEGKLAALVDTAKIPHPGRGANFVHPKFG
                     PVWSTGHLGDAVVSLISTASDDPKFAKYKEHNWKVVEQLKMPGAGNLFVKTHPKSKHF
                     WADAPMNPEREVAESVYVFDMNDLSKAPVALNVAKDSGLPESKAIRRAVQPEYNEAGD
                     EVWISLWGGKTDQSAIVIYDDKTLKVKKVITDPAIVTPTGKFNVYNTMHDVY"
     gene            20071..20964
                     /gene="cobA"
                     /locus_tag="PKN25_06515"
     CDS             20071..20964
                     /gene="cobA"
                     /locus_tag="PKN25_06515"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNV90585.1"
                     /translation="MQAGTFRSDASPAGAAKPRSLRASRAALPPRGTPEGIGERPRST
                     RAQGHGKVHLVGAGPGDPDLLTVKALRLITGAEVVVYDHLVGEAVVALIPPAARRLYA
                     GKEAGNHALAQHEINRLLVELAREGHDVVRLKGGDPFIFGRGGEEMQALLEAGVECEI
                     VPGITAAAGAASATGIPLTHREHAQTLVFATGHLKDDTVDLDWAALARPRQTVVVYMG
                     LGALEIICTQMISHGLAADTPAAVIHAATTPRQCGVHATLATLPGAVRAAGIRAPALI
                     MIGEVVALDPGALLAAAATAG"
     gene            21017..22459
                     /locus_tag="PKN25_06520"
     CDS             21017..22459
                     /locus_tag="PKN25_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HNV90586.1"
                     /translation="MAGENEFFRPIREIEQRRVVSCSADDALVDIVGRMREMSISCVV
                     VVDGAHPTAILTDRDLRNKVIAAGRDPAGLRVRDVMSAPVITIGEDDVLYEALYRMSR
                     HGIHRLVVVDRKGALAGIVTVTDLLRLQAHSPHQLVLDIEKAESIDALRELHQRIQQL
                     IVHLAGTGIAIRDTVKLIANLNDQVLIRLIDLLRRDRYPDLTTDFAFVVMGSEGRGEQ
                     TLSTDQDNAILYADSVQGEALARLEAFSNELIDALISIGVPPCPGGIMARNPAWRRSL
                     TAWKRELTQWLSTPTPDNVMTGSMFMDLRPLYGRRELADTLREHALHYMSHEQGFLVR
                     MAQNMVNFAPPLGWFGRIKVEKSGERRGEVDLKKAGIFAITDGIKALAMEARRLDGST
                     HDRMEALVEAGVLKPEQVADLRAAFDFLVRMRLRGQVEALRGGRKPGNYIALDQLNAM
                     QQGELRLALEGVASFQAFIQQHFKLQLLRN"
     gene            complement(22495..23409)
                     /locus_tag="PKN25_06525"
     CDS             complement(22495..23409)
                     /locus_tag="PKN25_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNV90587.1"
                     /translation="MTEARPSILQYWSAVIRTTVKLWLEAQVFVHAAALAFFTVFSVA
                     PVVIIAVSLVGLVLGESAAQGRIAEQLEVAIGPEVAAAIQTAVVNSRIQHSGLLPTIA
                     GVGAIVFGATTVFTQMQESLNAIWGVAPRPTRSGVFIHLKTRLLSLAVVLGIGFVLLV
                     SLSLSIFVRALVAFAQDWLPVPVGLIIGLDWAIALLVVTLLFATIFRVLPEVVLGWRD
                     VLPGAFVTALLFALGRALIAIYLSTTATASTYGAAGSLVLLLLWVNYSSLILLFGAAF
                     TRAYLEARGRTVRPRATAVLVHRQLIED"
BASE COUNT         3696 a         8300 c         7752 g         3775 t
ORIGIN      
        1 ttcctcggcg ctggcgtgtg gggcttcctg cacaccctgg ctccggtgaa ctacttcacc
       61 cacggctcgc agatcaccgc cgcgcacggt cacatggcgt tctacggcgc ctacgtgatg
      121 gtcgtgatca ccatgatcag ctatgccatg ccgatcctgc gcggccgcgc cgccaactcg
      181 accaaggcgc aagtgctgga gatgtgggcg ttctggctga tgaccatcgc gatggtcttc
      241 atcaccctgt tcctgaccgc cgccggcatc ctgcaggtct ggctgcagcg cgtgtccgac
      301 accccgatgt ccttcatggc cacccaggat cagctgatgc tgttctactg gatgcgcgaa
      361 tgggcgggcg tgatgttctt catcggcctg ctggcctatc tcgccagctt cttcgtgaag
      421 ggcgatgcca agggggtcgt gcacggctga gccctgccga ccgaaatgcg gtaacaagcc
      481 tggatcatcc agggtaaggg gttggggcgg cgcgagccgc ccctcttttt ttgcgtgcca
      541 cgctcgcagc atggagcggc cgctagaatg tcgggcttcg tccgcgcccg aacggcactc
      601 cgctgccacc ggatcccccc ggcgcgcgcg tcccatcgcc tccgctcgct cccctccgcc
      661 cgtgcccaac gcctcccttc cgccctcccc gccgtcccac gacgcacctc ccgtcctgca
      721 cccgagcggc gtcgagcgcc ccttccgcat cgcgatcaac ggctacggcc gcatcggtcg
      781 ttgctacctg cgtgccctgc acgaagccgg cctcgccgag cgctaccgca tcgtcgcgat
      841 caacgagcct gcggacctcg ccagcatcgc ctacctcacc cgcttcgact cgacccatgg
      901 caggtttccg ggggtggtgg accgcagcga gcgcgagctg atcgtggacg gtcatgcgat
      961 ccacgtgagc cacgcgagca ccccggaaga ggtcgactgg accgcgctgg ggctcgatct
     1021 cgtcgtcgag tgctccgggc aatacggcgg acgccgcgac ttgcagcgct tcatcgctgc
     1081 cggctgcccg cgactcctgc tgtcgcaccc cggcaacggc gccgaggatg tggatgtcac
     1141 agtggtgttc ggcatcaacg aggacgagct gcgcggcccc gggcgcctgg tgtctaacgc
     1201 gtcgtgcacc accaacgccg tggtcccggt gctcgactgc ctgcaccgcg agatcggcat
     1261 cgagcaggcc ctgctcacca cactgcattc ggcgatgaac gaccagccgc tgatcgacgg
     1321 ctaccaccac gccgacctgc gccgcacgcg ctcggcgatg cagtcgatca tcccggtgtc
     1381 gaccggtctg gcgcgcggcg tcgaacgcct gctgccggcg ctcgccggac gcgtccaggc
     1441 gaaggcgatc cgcgtgccca cccacaacgt ctcggcgatc gacatggtgc tgagcttcgg
     1501 ccgcgaggtc tcggccgagg ccgtcaaccg cctgctgcgc gagaccgccg ccgggccgca
     1561 gcgtggcctg ctcgcgtgct ccgacgaggc gcacgcctcg atcgacttca accacgaccc
     1621 gcactcggcg atcatcgact gcagccagac gcgcgcagtc ggagcgaaga tgctcaacct
     1681 ggtggtgtgg ttcgacaacg aatggggctt cgccagccgc atggtcgacg tcacccggcg
     1741 ctggctggcg gcggcgggct gaacacgaac gaatcccaat taaccacgat caagttaaac
     1801 ccgcgccctt ctgcctagac tgcgccgcat gaataccgtc accactccgc cgagttccct
     1861 gccggccggc ggaccggcac aggcgcacgg cccccgcctt gcgggcgacc tcgcgatctg
     1921 gttcttcatc ctcgccgagc tgctcgcctt cggggtcttc ttcgccgcct acgccttcgc
     1981 gcgcgccaaa aacatcgaac tcttcgccgc cgagcaggcc gcactcaacc gcaacgcggg
     2041 cgcgctcaac accgtgctgc tgctcactgc gagctacttc gtggtgcgcg ccgtgcaggc
     2101 cgccgaggcc caggcctcgc gacagtgcgc caactggctg ggcggcgcga tcctcaccgg
     2161 cctcggcttc atcgtcgtca agctgagcga gtacgccgca gcgttcgagc acaacatcag
     2221 cctctcgagc agcaccttcc acatgttcta cctgtcgctg accttcttcc acttcatgca
     2281 tgtgatcctg ggtctggtaa tcctgatcgc gatgtggaac ggcgcgcgcc agggccgcta
     2341 ccgcccgggc gagatgaacg gactggagac cggcgccgcc tactggcaca tggtcgacct
     2401 ggtctggctg atcctcttcc cgctcgtcta cgtcatccac tgaggagccc gccatgagcg
     2461 cacgcatgca cggaacgccc cgcggctcca cgatcctgtt cgccaccctg atcctcgcca
     2521 ccgtcgccac ctggggcatg ggctcgctcg gcgtgaccgg cacctggggc gtcgccatcc
     2581 tcgccgcgat ctcgttctgg aagggcgcgg tggtcatcct cgatttcatg gcgctgcgcc
     2641 atgcaccgct gctctggcgc gcgatcacga tgggctggat catcgtcgtc tgggcgctga
     2701 tcgccatcgc ctacatcaag ggactcgccc aatgaagccc gatacgccca acctcgacat
     2761 tcccttctac caacccgccg gcgacgaggt ggacatcttc cagcacgcct ggaagaaccg
     2821 cctgcccctg ctgatcaagg gccccaccgg ctgcggcaag acgcgcttcg tctcgcacat
     2881 ggccgcccgc ctcggcctgc ccctgtacac cgtggcctgc cacgacgacc tcaccgcctc
     2941 cgacctcgtc ggtcgccacc tgatcggcga cggcaagacg atgtggtgcg acggcccgct
     3001 cacgcgcgcg gtacgcgaag gcggcatctg ctacctcgac gaggtggtcg aggcacgcaa
     3061 ggacaccacc gtggtgctgc acccgctcgc cgacgaccgt cgcgtgctgc cgatcgaccg
     3121 caccggcgag ctcctcgagg cgccaccctc cttcatgctg gtggtgtcct acaaccccgg
     3181 ctaccagaac ctgctcaagg gcatgaagcc ttcgacccgc cagcgcttcg tcagcctgcg
     3241 cttcgacttc ccgcgccccg agcaggaaga ggcggtgctg atcggcgaga ccgcctgcgc
     3301 accggacctg gccaagcgcc tggttgccat cgccaacgcg ctgcgcgcgc tcaaggaccg
     3361 cgacctggaa gaggccgcga gcacccgcct gctcgtgtat accgccctgc tcgtcaagga
     3421 cggcatggac ccggtcgccg ccagccgggt cggcatcgtc gagagcctga ccgacgacgc
     3481 cgacgtcgcc gagggactga tggaggtggt cgccgccacc ttcggccgct gacggccggg
     3541 acatcgagat ggaaatcaag cgcgccgatc cgctccgcca cgtcatgccc ggcccgcgcc
     3601 tggcggtcgt cgccgaggcg ctgttcctga tcaacctgat gctcctgccc gggctggcat
     3661 tcctcgcgct gatggtgctg tggtacctgc atcgcgggca ccccgatccg ctggtgcgca
     3721 atcacctgcg ccagaccggg tggacctgca tctggggcgg gctgctgctg gtcgtgatca
     3781 gcgtcgcgat cttcctgctc ggcggcttcg acaatccgtg gacctgggtg atcggggtcc
     3841 tctatttcac cttcgtccac tcgagcctga tcctcctcgg cgtcatgggc ctgtcgcgcg
     3901 cgatcaacgg ccgcagctgg cgcttcccgg tgttcggacc gcgcgagccc tcctgagccg
     3961 gccggtgacc acgatgaaca ccggagcccc tgcgcgcaaa ccgcagcgcg tcatcccgat
     4021 cgcgaccgcc cctgcgggcg atgcggccgc cgccgcggcg atgcagcgcc gccggcgtgc
     4081 gttccaggcc ggcttcttcg cgctcttcgt gctcgcgccg gtattcgatc tcttccgcta
     4141 cgacctcgac gccgaccacg cctggctgct cggcatggaa tggcggctcg gcctcgaccc
     4201 cttcctcgcc ggccaggcga gcgcactcga ggcggccggg agcatcatcc tgaatctctt
     4261 cgtgcccctg ctcgcgctcg ccgcgctggt catcggcgcg gcgtggaagt ggggacggct
     4321 gtactgcggc tggctgtgcc cgcacttctc ggtggtggag tcgatcaacc ggctgatggc
     4381 gcgcgcttcg ggcaaggcct cggtatggga ccgccacctg ctgccggcgt ggaagcccga
     4441 cggccgcccc gcgcgggtgg acgcgcgctg gtggggcgtc gtcgtgccgg cggcggtggg
     4501 cttcgccttc ctgtgggcgg tggtgctgct cacctacctg ctgccgcccg cgcaggtgta
     4561 cggcaacctc ttcgccggcg agctcacgcg caaccaggcg atcttcctca ccgcggccac
     4621 ggtggtgctg agcctcgagt tcctcttcgc acgtcacctg ttctgccgct tcgtgtgcgc
     4681 cgtcgggctg ttccagagcc tggcctggat gggcaaccgc gacgcgctgg tggtcggctt
     4741 cgagcgcgag cgcgcgagcg attgctcgaa ctgcctgccc gaccgccagt ccgcctgcga
     4801 cgccgcctgc ccgatgcggc tgcgcccgcg caacatcaag cgccacatgt tcacctgcac
     4861 ccagtgccgc caatgcgtcg acgcctgcgc gactacccag cacgccaacc cgcaggggcc
     4921 gctgctgtcc tgggtggccg gcgaggcggc gcgccagaac gaggccgggt tccgcgcact
     4981 caaggatcgc tgacgccatg gaagaaaccg taggcctgct ctggcaccgc ctcatcaccc
     5041 gcgccgccgg cggacacttc cccaaggcgg cggtccgcct gcgcgacatc gagaagaccg
     5101 cgggcgtgtt cttccgcgcc ctcggcggcg accccggcct gcgcctggcg gccgccacca
     5161 ccgacgagca tggcgcgcgg cgcagcctgc tgcagcgcat cgccggcgcc gacgaacgcg
     5221 tcgcgcgcgc gcgcatggac gtgtccacgc tgcgcctgcc gcccgagctc gacgtgctgc
     5281 ccgagcgcgg cctcaaccgc gacctctacc tctggctcgc cgccgtggcc gccgcgcccc
     5341 ccgcggagga tggcgacggc caccccgcgc tcggccccgt gcccgacgag ccgatcgacg
     5401 aggccgccac ccgcgagctg cgcgagaacc aggccgccac cctgcgcgcc ctggcgcgct
     5461 ggccgggcat ccaggcacgc taccgccgcc tggtcgacgc ggtgatcgcc cagcgcccca
     5521 ggctcgacaa gctgccgccc gccgaggccg aacgcgagaa gctgatccgc ctcgccctgc
     5581 acgcccccgg cagcgtcgcc gccctgcccc gcctgcccgc caaggcgcgc gccacccagc
     5641 ccgtgctgct gtggctgagc gagttccacg ccagcgccgc gggcggcagc ggcggcgccg
     5701 gcgcgggcgg cgagctcggc gaggccggcg ccagccgtcc cggcaaggac agcagccgcc
     5761 aggcccaccg tgtcgagcgc caggagaaaa tgccggaaaa gcacggcatg atcatcccct
     5821 tccgcgccga gagcctgctg tcggtggcgg agttcatcaa ggtccagcgc agcaccgacg
     5881 acgagcccga cgacaacgcc gccgacgccg ccgccaacct cgaccacctg tcgatcaccc
     5941 gcgacggcga gcgcgtcgcc tccaaggtgc gcttcgacct cgacctgccc tcggctgccg
     6001 aggacgacgt cgtgctcggc gacggcatcc cgctgcccga gtgggactac cgcaagaacc
     6061 tgctgctcga ggaccacgtc cgcctcgccg agctcacccc ctcgatccac gacccgcgcg
     6121 ccgccccctg cgccctgccc gagcacctgc gccgcactgc gcgccggctg caccgccagt
     6181 tcgccgcgct cactccgggc agacgctggc tcaaggcgca ggtcgacggc accgagctcg
     6241 acctcgacgc ggtggtgcgc gccgccaccg accgcgccac cggccaccat ccgtccgatc
     6301 agctctacct ctcgctcgag aagcgcgagc gcgacctcgc ctgcctggcg ctggccgatc
     6361 tgtcgctgtc gaccgattcc tgggtctcct ccgaggcgcg tgtgatcgac gtcatccgcg
     6421 actcgctgct gctcttcggc gaagccctgc tcgccaccgg cgacagcttc gcgctatgcg
     6481 gcttctcctc ggtcaagcgc agcaacgtgc gcttccaccg cctcaaggac ttcgaccagc
     6541 gcttcgacga ccgcgcgcgc ggccgcatca tggcgatcaa gccgggctac tacacccgcc
     6601 tgggcgcggc gatccgccac gccaccacga tcctcgaccg ccagcgcgcc gcacgccgca
     6661 tcctgctgat cctgtccgac ggcaaaccca acgacctcga tctctacgac ggccgctacg
     6721 gcatcgagga cacccgcgtc gccgtcgtcg aggcacgcaa ccgcggtgtg gtgccgttct
     6781 gcgttaccat cgaccgcgaa ggcgcaagct acctgccgca cctcttcggt ccggccggct
     6841 acgcggtgat ccgccaaccc gacgagttgc ccgcacgcct gcccatgttc tatgcccagc
     6901 tcacccgctg aagccatgcc tcgtaacgcc accccggtcg gggaaagacc tgtccggatc
     6961 gattcctccc ccgccagccc ggtcacggag atcgaccgcc tgtgccggtt gctcgcgcgc
     7021 gcgccgcttt tcaacggcct cgcctgcgag gatatcgtcc gcttcgcgcg tggtgtgcgc
     7081 gaggtacggg tcgcgcgcgg cgagatcctg ttccatcgcg gcgacccctg ccacggcttc
     7141 cacctcatcc tcgacggcca gatcaagctc gccttcacct cggccgaggg caacgagaag
     7201 gtggtcgaca tcctctacgc cggacgcagc ttcggcgagg cggtgatgtt catggaaaag
     7261 ccctacgtcg tgatggcgca ggcactgacc gacgccaccc tgctgcacat cggcaagcag
     7321 atcttcttcg aggaaatgtc gggcgatccc ttgttctgcc gcaagatcat cgccggcctg
     7381 gcccagcgac tgcatctgct gatggccgac gtcgagagct actcgctgcg ttcgggtcgc
     7441 gaccgcgtgg tgggctatct gctgcgcgaa gaggagcgtg acggcgacgg ggtggccgaa
     7501 ggctcggtgt cgttgcgcct gcccaccagc aagggcacga tcgcctcgcg gctcaacctc
     7561 acccaggaac acttctcccg catcctcagc gaactcacca gcgcaggcct catccaggtc
     7621 gaaggccgca ccatccacat ccccgacatc ggccgcctgc gccgaagcct gggttgaggg
     7681 tcacgccttt cagcgcgtca aacccgcacc cacgcttccg gaacgtcgca atcgcgtctc
     7741 gcatcgctcc tgcagggcga tccttgagcc gtcgtgaatg tccgggcgat gcaaaagggg
     7801 cccgcaggcc cctttcctgt ttgtgggcgc cccgcaagcg ctcaaccctc cttgccgtcc
     7861 acccgcgcat accagaggta gggggcgtag cgccacaggt agagcaggaa ggcgatcgac
     7921 cacagcgtgg cggccgcatg cacgccgccg aactgtgcgg ccgggaagaa gcccacggtc
     7981 agcacccgca ccagcgcggc gatcagcacc agggcgtagg ccgcggtctc gatgcgaccg
     8041 gcgatcagca tgcggccggt gtggccgagc gcggtgcggg taatcatgcc gatgatcagg
     8101 ccgccggtcg cgccgatggc cagggcatgg actccggccg agcgcggcag cacgccgaac
     8161 tgggtaagcg cgagcagcgc caggccgagc ggaatccaca gataggccag ttgcagcacc
     8221 cacaggatgg gatggccgcg cgtcgcccaa gggttccagc cgcctagccg caccgcctgc
     8281 agaacggctg cgatcaacga caccacaccc gcccagcctc cggcacccag cgcccacagc
     8341 agcagcgcga taccggtggc gatcgccgcc gcccagtcga ccttcacgtt gcgccactgc
     8401 ttgacgccac gtaccgcgtt gaagatgaac atcgggatca cgcggccgcc gacgatggtc
     8461 tccagcacca ccaccaggcc gagggccagg tgcatggcgg tcagcggatc ggcctcgatc
     8521 acgccgagca cggcgaggtg gaagaacagg ttcgcgagcg cgagcatcgc gggcagcgca
     8581 ccgacgaagt agttgcgctt gctcttggcc ttgatcagca ccttcagcag catccccgcc
     8641 gcgaccggaa ggaaggccag atcgacgatg gccacccagg ctccgctgcc gaaggccatc
     8701 gccaggcgcc cggcgagcca caccgcggcg agcaccatga gcggcttgcc cgtcggggtg
     8761 tcgagcccgg tccagttgcg ccctgcggtg aacaggaagc cgatgatcac cgccacggcg
     8821 aagccgaaga tcatctcatg cgaatgccac cagattcccg gcatgggcag gtcgatcgcg
     8881 cccgagtagg ccaccgccca aaccggcacc gcgatcgctg cgaacagtgc ggcgagcagg
     8941 tagaagggac gaaaggcgag cgcccacagc gagaaggtgt cgggcggaat gcgtttggtg
     9001 gtgggttcgt cgaggcggat cagcgccatg ttcggttctc ttttctgctg aacgggttga
     9061 aggcggatga agctgcgcac ggccgcacac ctgcccggcc gggaggaatc agtggctggt
     9121 gccttcctcg cgcgtgatct tgttccatac gttgtacttg cccacaggct ttctcatcgg
     9181 caggcgcttg acctcctcga gggtccgcgc gtcatagacg atcaccgcgc cctcgtcctc
     9241 ccacaggctg gcgagcgcat ggcggccatc gcgggtgaac tcgacgtggg cgagcgtctt
     9301 gcccggctca ccggggatct cgtgcaccac ctccagcgtc tgcttgtcga tgacctgcag
     9361 gatgtgcttg gcctccgcgc tcatcatcga atcgacgaac gcatagcggc tgctggagtg
     9421 gctgcgcagg aagaagcccg ggccgcgggt ggggatgcgc ttcaccacct cccaggtctt
     9481 catgtcgatc acggtgacct cgcccgcctt gaggttggtg ctcgccatca ccgtgcgcgg
     9541 cctggcgccc gggccggcgg cggggtcctg ccattcccag gtgatgcccg agcccaggtg
     9601 cggcatgccg tcaagcggca gattggcgat cttcttgcgc acgtcgaggt tcaccacctg
     9661 gccgaccccc tcgcgcgagg cccccatcag ctcggaatag tcctgggtga agaagaagtc
     9721 gtcgagcacc tcggcgagga tggtgcggcg cgggttgagg taaccgggaa tgaagctgcc
     9781 ttcgcgctgt gtgtagtcgt ggatgtagct gaccgggatg tcctcgaccg ccttggtgta
     9841 gctgatctcc cacacctcgc cgacgtcctt gagcgcggcg atgaaggact ggcgcggcgt
     9901 ggcgtcgtac accgccgaca cccgcgagct cttcttgccg tcgcggtcca tcgccgggat
     9961 ggtcttgacc aggttcagct cgccgtcgag cagcaccagc gtgttgggga ggtagttggc
    10021 caccgcgacg tgggtgccgt cgggcgatgc ggcgacgttg cgggtgttga tgccggcacg
    10081 cacctcggcc acgaccttca ggttccacag gtcgaacttg gtgatccagc cgtcgcgcga
    10141 ggcgaagaac acgtagcgcc cgttggcggc gaacttgggc ccgccatgca gcgcgaagcg
    10201 cgactggaag cggtggatcg gctcgagcct gtcgccgtcg agcacgctga cgtggtggtc
    10261 gcccgcctcc accaccagga agaggttgag cgggtcggcg tcgaacaccg gcttgtcgga
    10321 aagggtcgcg gtgtcgacat ggacgacgcg cgaggcgcgg atgtcctcgt cggtccagcg
    10381 cggctcgggc accaccgggg tgtagatcca ctcggtgagc gcggcgatct cgtccttgcc
    10441 cagctgcgcg gcgaagccgg ccatctgggt cgcgggccgg ccctcggcga tcaccttggc
    10501 cgcctcgctc ttgcgcaggc ggccaaggtt gtcgggcagc agcgcgggac ccatcgctcc
    10561 gaggcgattg ggcgcatggc aggccgcgca atgctgcgcg tagagcgcgg gtacgtcgat
    10621 ggcagccggc gacgccgctg ccccggacgc ggcccgagcg acggccttgt ccggcgaagc
    10681 ggcggcaggc aagacgacca gtgcaccggc gagtgcgaac aggccggtca gggtcgaggc
    10741 gcggatcttc atgcggaaac cttgtcgatg cgggtccagg gcgtcgtgac gacgcgctcg
    10801 gcgccgtagt ctgccggatt cacgccgatc tcctcgtcgt agaggtagca ggccgggtcc
    10861 tccgcccacg ggttgccggt gacctgctcc gcgcgcacgc gcgagctgcc gttgcagatc
    10921 tccaggtact tgcacgcgcc gcagcgcccc tccagcttgc gcggatgctg cttgaggccg
    10981 gcgagcagcg ggttcgacag gtcgttccag atctcgccga aggcgcgctg gcgcacgttg
    11041 ccaagcggca cgtgccacca catggtgtcc gggtgcacga tgcccaggtt gtcgatgttg
    11101 gcgacgttca ccccgctggc gttgccgccc cactggcgca gcctggcctc gatgtgggcg
    11161 gccttgtcgg ggaagcggcg ctgcacccag tgcagcagga aggggccgtc ggcatcgttg
    11221 ttgccggtga cgaagtcctt gtcgatgcca cgccggtgca ggtcgagaca ggtctcgaaa
    11281 agcagctcca tggcctcgcg ggtggtgcgg tggcgggcgt cgccggcgcg gttcttgttg
    11341 ccgcgcccgg cgtagttcag gtgcgagaag tagaacttgt cgatggcctc gtcctcgacc
    11401 agcttcaaca gcgcgggcag gtcgtgggcg ttgccctcgg tcatcgtgta gcgcacgccg
    11461 accttgatgc cgcgctcgcg gcacaggcgg atgccgtgca tcgaggcctc gaaggcgccc
    11521 tgcttgcggc ggaagtggtc gtgcgtggcg ccgatgccgt cgaggctgac gccgacgtag
    11581 tcgaagccga tctcgtcgat cgcatcaatg ttggattcgt cgatcagggt gccgttggac
    11641 gacaggccga cgtagaagcc catacccttg gcgcggcggg cgatgtcgaa gatgtcgggg
    11701 cgcagcagcg gctcgccacc ggacaggatc agcaccggca cgcgtgcgtc cttgaggtcg
    11761 tccatgacct tgaagacttc ctgtgtgttc agctcgccgg ggaaatcctt gtccgccgag
    11821 atcgagtagc agtgctcgca ggtgaggttg cagcgccgga tcaggttcca gatcaccacc
    11881 ggacctggcg ggttgcggcg ggggccggca ggcgtggggt ggtcgagctc gcggatgaat
    11941 tgggagatgc ggaacatggg agcctcctga atgctggcca tttcacccga ggcgcccgcg
    12001 aatgaaaatg atgcgcgtca agggtgcccg gggcgggcgc tcgtgccggc cggaagagtc
    12061 gcttgccgga tcaggcgccg atgcgcaggc ccgtcttttt caggatgccg gacgagaaca
    12121 gcacgtcgcg gttgcggcag gcaccaggaa actccgccgc gatcagcgcg gcgatctctt
    12181 ccacgcgcgc gagcgcggcc tcgtggctgg cggcgtgcac catcgcgaac aggttgtagg
    12241 gccagtcggg cagatggcgc gggcggcggt agcagtgggt gacgaagtcg agcgcaccga
    12301 cgcgctcgcc gaccgcgtcg atcaccgcgg cgtcgatgtc ccacacgctc atgccattgg
    12361 cggtgtagcc gatggcgtag tggttgggca ccgcgccgat gcggcgaatg cggccgtcgg
    12421 cgagcatggc ggcgaggcgc tcgcgcacgc ggccctcggc cacgccgagc gtctcggcca
    12481 gcgcggcgta ggggcgtgga accagcggca ggccgccctg ggtggcgagc acgaggcggc
    12541 ggtcgaactc cgcaagggca gcttcatcca tgccggcacc cgcggcatcc tgcgcatccg
    12601 cccgcgcgag ggctccgttc ccggacatgc cttgcaccat caggcctcca gcctcatctc
    12661 gacgaagtac tcgcgttcct tggggaacag atgcaccacc aagccggtag cggcctcgat
    12721 gcggcgcgcg cagtcggcga tgccctccgg cgtctcggtg gcgagcacga accacatgtt
    12781 gagcgcgtgc tcgcggcggt agttgtgcgc gacctcgggg aaggcattga ccgcctccac
    12841 cgcgctcgtc cactgcgcct cgggcaccgc gatcgcggcc aggcagaagg cgccgcccat
    12901 gcgctcgatc tggtacatcg gcccgaaacg ggtcagcacg cggtcgtcga gcagcgactg
    12961 caggcgcgcg atcacctcgt cctccgccag gccgagcagg acgccgacct cggcaaaggg
    13021 gcgctcggag agcgggaact cgccctggat ggcattgatc aggcgacggt cggtgtcgtc
    13081 gggcgcacgc ttcgcggcca cgccacgacg gccgaccgac gcggccggct gggcttcaga
    13141 catgggcgag atccttggcg tcgagatagc gcgcgccggt ctgcttgaaa cgccggcaac
    13201 tgaacagcac cgcgtgcgga tggcggtcga ggccgaggcg cgcggcgaga tccgcccgcg
    13261 cggccagcac ctcctcgcgc gcgctgccgt ggatcatgca gaacaggttg tacggccagc
    13321 cgggcagcac gcggcggcgg cggtagcaca gcgtgaccgc cgcctcctgc gcgagccggc
    13381 gaccaagccc ggagacctcg gcgtcgggga cgtcccacac gcacatggcg ttcgcctcga
    13441 ggccgagctc atggtggcgc acgaccacgc cgaagcggcg gatcaagccc tcgtccaccc
    13501 agcgcgcgat gcaggcgatc acctcgccct cggtgaggcc gacgcgcgcc gccagcgtgg
    13561 cgaaggggcg cgcgtccagc accaggccgc cctgcagcgc gccgatcagc ttgcgttcga
    13621 gcgcaggcaa cgcgcagccg ccgtcggcgt cgccccgtgc gatcggctcg tcgctgcgcg
    13681 cgtcggcatg cggtcgccgc ccctccccgc ccgccttgag gtcgaagccc aggtcgatgt
    13741 ggaactcttc ttccagcggc agcacgatga ccgcacagcc ggtgtcgcgc tcgatgccgg
    13801 cgaccacctc ttcgagttgc gcacgcgagg ccgcggtgac cacgaaccag aggttgtagc
    13861 gatgctcgcg ctggtagttg tggttgatcg ccggctcgcg cgtgacgcgc gcggcgacct
    13921 cctccaggcg ctccggcggc gcggccaggg ccgcgagtgc gctcgcaccc aggcgacgcg
    13981 gggcgaacac cgcgcccacc cggctcacca ggccttgcgc gctcaggtcg cggtaggccg
    14041 cgatgacgtc ttcctcggcg gcgcccaccg cctgcgcgat gcgtgcgaaa ggtcgcggct
    14101 cgagcgggaa gtcacgctgc cactcgttga gcaggcggaa gccggtggcg tcgagcgccg
    14161 cgggcaggat ggcctggcgc ggcatcagaa gcccgtgcgc gcggcgcgcg tggtgaagaa
    14221 gatgccgctc gggctcgccg actcgaagcc gccgatcggc tgcttgctgg cggtgtcgta
    14281 gatcacgacc ttgttgtcgt cgcgcgccga cagccagatc tcgtggccct tggagaggaa
    14341 ctccatgtgc aggatgccgc ggcccggctg cagggtgtcg gtgaccttgc cggagacggt
    14401 gtcgatgacc tgcacccagc cgttgtcggg gaaggcgaag ttcacccaga tctcgcgccc
    14461 gtcggggcgc gccatggcga acaccggctg gctcttcacc tggatgcgat cgacctcctg
    14521 ccaggtggcg gtgtcgacca ccagcacctc gtggcgaccg atggcgggca ggtaggcccg
    14581 attgccggcg atcgaccagc cgcgcaggtg gggcatcttg aacaccggca ggggctgctc
    14641 gccacgaccg tagccggaga ggatcttgcg gctgcccttg tcgaggttcc acaggtcgat
    14701 cagggcaagg ccgtcctcgc cgaacaggcc ggcgatgtag taccggccat cgggcgtgac
    14761 cagtgcatcg tagggctgct tgccgccggc aaaacgccgg gtcagcggat tcttcgggtc
    14821 ggagaagtcg gtgatgcgaa tctcgcccgc ctcgaacagc gaatagatga agcgcttgcc
    14881 cgagaggtcg gcgaggccca ccaccttgga gcgggtgccg tcgtccgtgg tcgcaggcac
    14941 atcggcgacg agctcgagcg tgtcggcgtc gaaggccttg atgccgcccg gttcgtagtt
    15001 ctgcaccacc accaggctgc cgtcgtgcga gatcgagccg ccgatcgcat tgcccgcctg
    15061 gaccttgcgg ccgacgatcc ggcgcgccag cagatcgacc ttggtcagcc cgccgtcgcg
    15121 cccgaacacg aaggcgtagc ggccatcgcg cgagaaaacc accgaggcgt gggagagatc
    15181 gcccaggcca tccacggtgg ccagcacgct gcggccagtg gtctcgatca ccttgacctt
    15241 gccgtcggca cgctcgatca ccacgccgag gtcgccggtg ccgcgcacgg cgaccggcgg
    15301 cacgctcgaa caggccgaca gcagcacgac catggcggag aggaccacgc cccatagggc
    15361 gggcggtgcg agacgaactt cacggcaaga catgggagct ccgggaactt ggggtggcgg
    15421 agggccaccg ggaagactgc cgtccgcact cgccaacagg ggcgtggcac gggggccgta
    15481 cgacggacgg cgatggttca gttgcgcgcg ggggcctgcg gcaggccggc atcggcggga
    15541 aaaccgcgca tcagccaata cacgatccac tccgcctcgg cttcggagag gatggactgg
    15601 aaaggcggca tcggcgtccc cggacggcca ccgacgatgg tggccacaag gccctcggcc
    15661 ggcttgtcgg caagcgcctc ggcggtcagt gcggggccga gcccgccctt gaaggtcagg
    15721 ccatgacagg aaccgcaatc ctggcggacg atgtggacca gttcgcgcgc acgcgccgga
    15781 gcgggctcga ccgggacgtg cccggaggct gcggcatagg cggagacgag cgctgcgccg
    15841 aggaagacga cggcgtgggc gaggagtggc ggaatcttca tgctggcatc cgacggtaag
    15901 gcatcggtga agagcatggg gcctcgaccg ggtcgaaacc ttgactcgca tcaacctcga
    15961 agggcgaggg gtctccgccg cacccgctgc atccgctcac gcaccctttc cacctcgacc
    16021 gcccgggccc acgagcgaaa tccgtcggcg agccacgcgc gcatcattcg gcgacgatca
    16081 ccacgccgag catttccgga tgcggtccgc agcggtactc gaagcgcccg gcctccggga
    16141 aggcgcgcga ccacttctcg ccgggaaaaa agcgctccga ctcctcgttg cggccgatca
    16201 gcagcaccga atgactcacc cgcttttcgt tatttaccca ggtcacggtg tcgccacgct
    16261 tgatgcgcaa ctccatcggc gaaaaggcgt atttctccac cacgacctga tgttcggcgg
    16321 cttgcgccgg aacggccgcg gaaagcccga ttgcgaccgc gaacagactt gccggaacga
    16381 gtctggcgtt catggaaatc tcccgtttca tgcccacgcg accacgcagt cgcgatggat
    16441 ccaccgcaaa ggacaacggc ggaagccccg ggttcgccgg atgcttccgc cgtatcgaac
    16501 accgcccagg agcacgggcg gggtcggacc ggattactgc ttggccgcct tgatgtggcc
    16561 gtcggtgtcg aggccgagct tgtagccgag cgaaacgtgg tggaaacgac cggccgcgtt
    16621 gtcgtcgtgg atcgcgaagc cgaagttgta ggccttgccc gactcgagct tcacgtcacc
    16681 gtcacccgca gcgagcttgc gggtgaacac caccgaccag ttgtcgccgt ccttcttgcc
    16741 ttcggcgctg accagggcgt tgccgccctc catcacgcgc ttgtcggcga cgtagccgtt
    16801 gtagcccttg ttctcgccgc tgcgccactg gtagaggtcg tagaacttgc cctcggcaac
    16861 cgagccgccc ttgacgtact tggtcttggc atcgtccgca cccggcatgg tgcgcgaatc
    16921 ggtatggcag gtcgcccagc agcccgcttg atcggcgagt tcgaccttgc cgcccgactc
    16981 gagcatgaac gcgatcttga ccgggttctt gtcgtccatc ttggcggcgc ccgaggcggc
    17041 cggctgcttc caggagaagc ggatgtagag gttctcgccg tcgtgcgcag cctgcacctt
    17101 caccggaatg aaggcggcct tgccggggat cgggctaggc tcgatcttct ggccgctggc
    17161 catcttcttg cccatgtcgc ctgcctcgtc gctgtggcag tcggcacagg tctcgccctt
    17221 cttgagcgca cgcgcccccc cgtgctcggt gcccttctgg atccactcca gcggcgacac
    17281 gccggggtag aaggcggtga tgtccttgcc ctgcaccttg ctccagtcgg gcgcggctgc
    17341 gagcgcgcta cccgtaccga tagccaggag agcaccgacg gcgccggcaa tcaggttctt
    17401 cttcatcgtc atatcctcgc tgtgcttggg ttggcctgtg atccacgagg gatcttactc
    17461 ctcgtccgcg tccgtcatgc cttccggctt gtggtgggcg atgcccttgt ggcaatcgat
    17521 gcaggtcatg ttgtcttcct tcgccatctc gtgctgcttg cgcgcacgct gcttctgggc
    17581 ctcgggattc atgctctcga aactgtggca gttgcggcac tcgcgcgagt cggccgcctt
    17641 catccgcttc cactcgttgc gcgccagttg caggcgcttg gcctcgaact tctcgcgcgt
    17701 gtcgatggtg ccgaccatct tgccccacag ttccttggac gcctgaacct tgcggatcat
    17761 cttgtgcgtc cagtctttgg gcacgtggca gtccgggcag gtcgcgcgca cgccggtccg
    17821 gttgttgtag tgaatggttt ccttgtactc catgtacacg ttgtcataca tctcgtggca
    17881 cgaaatacag aacttctcgg tgttcgtggc ctccagcgcg gtgttgaagc cgccccagaa
    17941 caacacgccc accatgaatc caacagccaa caggccgccc agcgacttac cggaaggacg
    18001 gcgcaggcgt gcccacaggc cgcccggatt ttcttgtttg gagttgtcgc tgttttgatc
    18061 agacatggaa ctcttcccct cgatcccccg cgccaggggc gcgggggttt ttcagagctt
    18121 gaccggaaga tcgatcaata gacgtcgtgc atcgtgttgt acacgttgaa cttgccggtc
    18181 ggcgtgacga tggccgggtc ggtgatcacc ttcttgacct tcagcgtctt gtcgtcgtag
    18241 atcacgatcg cggactggtc ggtcttgccg ccccacagcg agatccacac ttcgtcgcct
    18301 gcctcgttgt actccggctg cacggcgcgg cggatggcct tgctctcggg caggcccgag
    18361 tccttggcga cgttgagcgc gaccggcgcc ttcgacagat cgttcatgtc gaacacgtac
    18421 accgactcgg ccacttcacg ctccgggttc atcggcgcat cggcccagaa gtgcttcgac
    18481 ttcgggtgcg tcttgacgaa caggttgccg gcgcccggca tcttcagctg ttcgacgacc
    18541 ttccagttgt gctccttgta cttggcgaac ttgggatcgt cggaagcggt cgagatcagc
    18601 gagaccacgg catcgccgag gtgacccgtc gaccacaccg gaccgaactt cggatggacg
    18661 aagttggcgc cacgccccgg gtgcgggatc ttggcggtat cgaccagcgc agccagcttg
    18721 ccttccttgg tgtcgaccgc ggcgaccttg ttggacgcgt tggcggcgac catgaagtag
    18781 cgcttcgaca tgtcccagcc gccatcgtgc aggaacttgg cggactcgat cgcgatgctc
    18841 ttcaggttct tgatgtcgga gtaatcgacc agcaggatct ggccggtttc cttgacgttg
    18901 accacccact ccggcttcgt catcgaggcc acgatggagg ccacgcgcgg ctcggggtga
    18961 tagtcgccat cgacggtgtt gccacgggtc gacacgatct tcatcggctc gagcgtctcg
    19021 ccgtccatga tcgagtactg gggcggccag taggtgccac cgatcaggta cttgtcctcg
    19081 tagcccttga acttggaggt atcgaccgaa cgggcatccg aacccaggcg cacggtggcg
    19141 accgtggtgg gctcttcgta gaacatgtcg atgatggtgg tcaggccgtc acgaccgacg
    19201 gtgtagacgt agcggcccga agccgacagg cgggagatgt gcaccgcgta gccggtatcg
    19261 aggatcttcc agatcttgtg ggtgtcgccg tcgaccagcg cgagcttgcc cgcgtcgcgc
    19321 agcgtgatcg cgaacacgtt cttcaggttg accttgttca tctgcttggt cggacggtcc
    19381 ttgacgggga ccagcagctt ccagctgtcc ttcatgtcct tgagcgagaa ctcgggcggg
    19441 atcggaggcg tgttctggat gtaacgcgcc atcaggttga tctcttcctt ggtcaggatg
    19501 tcatcgtagt tgaccatgcc gccttcggtg ccgtacgaga tgatcttctc gaggcgctcg
    19561 gtgccgagct tgagggtgcc gccctccagc ttggtgccgt ccggagcggt cttggtccag
    19621 tgcggctcga ggttcttgcc ggtggcgccc ttgcgcagca cgccgtggca acctgcgcag
    19681 cgctcgaagt agatctgctt gcctttttcc atctcctcgg cggtcagctt gggcgcttcc
    19741 tgggcccagg cgctgctcat cgcgagggac agcaccccca tggcaaacgc cgatccgatc
    19801 aaacctttct tttgcatagc cctctcctta cacgacacgg gatgtgaaac aacattgcga
    19861 cggcagtatg gtttttccgg ggcaggccca tccttgatgt gaattaattt tccacaccag
    19921 cgccgaggat aaagttcggg gacgccggaa cgacgtcgcc atgcgcacgc ccgcggattt
    19981 cgcggtgtcc tggacaagcg ttttccggcc cctggcggaa ccgatttttc gccgcaacgg
    20041 atttcccgag accgtgctgg agcccacatc atgcaggcag gaacctttcg atcggatgct
    20101 tcgccggccg gcgcggccaa gccgcgctcc cttcgcgcca gtcgcgccgc cctgccgccc
    20161 cgcggcacgc ccgagggcat cggcgagcgg ccgcgatcca cgcgtgcgca gggacatggc
    20221 aaggtccatc tggtaggcgc cggcccgggc gaccccgacc ttctcaccgt gaaggcgctg
    20281 cgcctgatca ccggcgccga agtggtcgtc tacgaccatc tcgtcggcga ggcggtggtg
    20341 gcgctgatcc cgcccgccgc gcgtcgcctg tacgcgggca aggaagccgg caaccacgcc
    20401 ctcgcccagc acgagatcaa ccgcctgctc gtcgaactcg cacgcgaagg gcacgacgtg
    20461 gtgcgactca agggcggcga tcccttcatc ttcgggcgcg gcggcgaaga gatgcaggcc
    20521 ctgctcgagg ccggcgtcga gtgcgagatc gtgcccggca tcaccgccgc ggcgggcgcg
    20581 gcctcggcca ccggaatccc gctcacccac cgcgagcacg cgcagaccct ggtgttcgcc
    20641 accggccacc tcaaggacga caccgtcgac ctcgactggg cggcgctcgc ccgcccgcgc
    20701 cagaccgtgg tggtctacat gggcctcggc gcgctcgaga tcatctgtac gcagatgatc
    20761 tcccacggac tggcggcgga cacgccggcg gcggtgatcc acgccgccac cacaccgcgg
    20821 cagtgcggcg tgcatgccac gctcgcgacc ctgcccgggg cagtgcgcgc ggccggcatc
    20881 cgcgcgccgg cgctgatcat gatcggcgag gtggtcgcac tcgaccccgg cgcgctgctc
    20941 gcagccgcgg cgacggcggg ctgaaacggc cgcgcccggg taaaatggcg aattcgacgc
    21001 accccgggga gcagccatgg caggagaaaa cgagttcttt cgtccgatcc gggagatcga
    21061 gcagcgccgc gtggtgagct gcagcgccga cgacgcgctg gtcgatatcg tcggccgcat
    21121 gcgcgagatg agcatctcct gcgtggtcgt cgtcgacggc gcgcacccga cggcgatcct
    21181 gaccgaccgc gacctgcgca acaaggtgat cgccgctggg cgcgacccgg cggggctccg
    21241 ggtgcgcgac gtgatgagcg cgccggtgat caccatcggc gaggacgacg tgctctacga
    21301 ggcgctctac cgcatgtcgc gccacggcat ccaccgcctc gtcgtggtcg accgcaaggg
    21361 cgcactcgcc ggcatcgtca ccgtcaccga cctgctgcgc ctgcaggcgc actcgcccca
    21421 ccagctcgtg ctcgacatcg agaaggccga atcgatcgac gccctgcgcg agctgcatca
    21481 gcgcatccag cagctgatcg tgcatctcgc cggcaccggc atcgcaatcc gcgacacggt
    21541 gaagctgatc gccaacctca acgaccaggt cctgatccgc ctgatcgacc tgctgcgccg
    21601 cgatcgctac cccgatctca ccaccgactt cgccttcgtg gtgatgggca gcgaagggcg
    21661 cggcgaacag acgctatcga ccgaccagga caacgccatc ctttacgccg actcggtgca
    21721 gggcgaggcg ctggcgcggc tcgaagcctt ctccaacgaa ctcatcgacg ccctgatctc
    21781 gatcggcgtg ccaccctgcc ccggcggcat catggcgcgc aaccccgcct ggcggcgcag
    21841 tctgacggcc tggaagcgcg agctcacgca gtggctatcg acgccgacgc ccgacaacgt
    21901 gatgaccggc agcatgttca tggacctgcg cccgctctac ggtcggcggg agctcgccga
    21961 caccctgcgc gaacacgcgc tgcactacat gagccacgag cagggcttcc tcgtgcggat
    22021 ggcgcagaac atggtcaact tcgccccgcc gctgggctgg ttcggccgca tcaaggtgga
    22081 aaaatccggc gagcgccgcg gcgaggtcga cctcaagaag gccggcatct tcgccatcac
    22141 cgacggcatc aaggcgctgg cgatggaggc gcgccggctc gacggcagca cccacgaccg
    22201 catggaggcg ctggtcgagg ccggcgtgct caagcccgaa caggtcgccg acctgcgcgc
    22261 cgccttcgac ttcctggtgc ggatgcgact gcgcggccag gtcgaagcct tgcgcggcgg
    22321 gcgcaagccg ggcaactaca tcgccctcga ccagttgaac gcgatgcagc agggcgagct
    22381 gcgcctcgcc ctggagggcg tcgccagctt ccaggccttc atccagcagc acttcaagct
    22441 gcagctgctg cgcaactgag cggagcgcgg ccgagccacc cacgggaggc ggactcaatc
    22501 ctcgatcagc tggcgatgca ccagcaccgc cgtcgcgcgc ggccgcaccg tccggccgcg
    22561 cgcctccaga taggcgcgcg tgaacgcggc gccgaagagc aggatcaacg aggagtaatt
    22621 cacccacagc agcagcagca ccagcgagcc cgccgccccg taagtggacg cggtcgcggt
    22681 ggtcgacagg tagatcgcga tcagcgcgcg cccgagcgcg aacagcagcg cggtgacgaa
    22741 ggcgcccggc agcacgtcac gccagcccag caccacctcg ggcagcacgc ggaagatggt
    22801 ggcgaagagc agcgtcacca ccagcagcgc gatcgcccag tccagcccga tgatcagccc
    22861 caccggcacc ggcagccagt cctgcgcgaa ggcgacgagc gcgcgcacga agatgctcag
    22921 cgacagcgac accagcagca cgaagccgat gccgagcacc accgcgagcg acaggaggcg
    22981 cgtcttgaga tggatgaaca cgccgctgcg cgtgggccgc ggcgccactc cccagatcgc
    23041 gttgagcgat tcctgcatct gggtgaatac cgtcgtcgcg ccgaacacga tggcaccgac
    23101 gccggcgatc gtgggcagca gcccgctgtg ctggatgcgg ctgttgacca ccgcggtctg
    23161 gatcgcagcc gccacctccg gcccgatcgc cacctcgagc tgctcggcga tgcggccctg
    23221 agccgcactc tcgccgagca ccaggcccac caggctgacc gcgatgatca ccaccggcgc
    23281 caccgagaac acggtgaaga acgccagcgc agccgcatgt acgaagacct gggcttccag
    23341 ccacagcttc acggtggtcc ggatcacggc ggaccagtat tgcaggatcg acggtcgggc
    23401 ttcggtcatg gccgcatgtt agcgccattg ccctggaagc cgcttccaga acgcgcccct
    23461 cagctcactc ccactcgatt gtcgccggcg gcttgcccga gatgtcgtag accacccggt
    23521 tga
//
LOCUS       DAOQBS010000040        23360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1423_length_23360_cov_16.386612, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000040 DAOQBS010000000
VERSION     DAOQBS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 23360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23360
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1423_length_23360_cov_16.386612"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKN25_06530"
     CDS             <1..366
                     /locus_tag="PKN25_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV90588.1"
                     /translation="VRAEMLEVLRTDYIKFARARGLTDRTVHFGHALRNTLLPVITIT
                     GLQIGSIIAFAIITETVFQWPGVGLLFITAVQFVDIPVMAAYLVLIALIFVVINLIVD
                     LLYFVVDPRLRVQRTGRGQ"
     gene            393..1352
                     /locus_tag="PKN25_06535"
     CDS             393..1352
                     /locus_tag="PKN25_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV90589.1"
                     /translation="MSTESPTPTTAAAPRGALARLLDSDLLHGFLRSPITVLSALIVL
                     AILIAALLAPVIAPQNPFDPATLNLMDGFSRPMQANAFTGNVYWLGTDAQGRDLFSAI
                     LYGSRVSLLVGFAAVAFAAVLGIALGLIAGYRGGWVDSLIMRIADVQLSFPAILVALL
                     IFGVFKGVVPPALHDKAAIYVLILAIGLSDWVQYARTVRSSTLAERNKEYVQAARVIG
                     VPAPTILLRHILPNVMGPVLVIATIGLALAIILESTLSFLGVGVPPTQPSLGTLIRVG
                     QDYLFSGEWWIVFFPGLTLLLLALSVNLLGDWLRDALDPRLRR"
     gene            1349..2380
                     /locus_tag="PKN25_06540"
     CDS             1349..2380
                     /locus_tag="PKN25_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90590.1"
                     /translation="MNVPHIDRMNAGGDLPLLSVRNLRVEFPRRRGTLVALDDVSFDI
                     ARGEVLGVVGESGAGKSLTGAAIIGLLEPPGRIAGGDILLDGEAIHALRGEAMRRLRG
                     RRIAMIFQDPLTSLNPLYTVGEQLVETMLTHLDLTPAAARERALALLDEVGIPAPAQR
                     IDHYPHQFSGGMRQRVVIALALCAEPELIIADEPTTALDVSVQAQIIALLRRLCRQHR
                     TAVMLITHDMGVIAETADRVAVMYAGRVVEIGPVAEVVRAPAHPYTRGLMGAIPVLGA
                     EVERLVQIDGAMPRLDAIPSGCAFHPRCTEASARCRVERPELLPAGATRAACWLYAPA
                     RENTDGGPQ"
     gene            2377..3657
                     /locus_tag="PKN25_06545"
     CDS             2377..3657
                     /locus_tag="PKN25_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV90591.1"
                     /translation="MSIIERALAKAKQEDRAAVEQAEGLVHRPQAGQDAQGAVAERTG
                     DVADHPAVAAAVEAEIEPAALAPAPAAAGARRVRDARAAAPLLYVEDLRCAFDVSRPW
                     LERVLAREPRRWLRAVDGVSFAIGRGCTFALVGESGCGKSTVARLIVGLHEPSAGRIV
                     FDGRDVAEVRGSAERQQLRSRFQMIFQDPYASLNPRWRVERIIAEPIRVFGLERDEAA
                     IRARVAALLGQVGLSPADGAKYPHEFSGGQRQRICIARALANRPEFLVCDEPTSALDV
                     SVQAQILNLMRDLQDELGLTFLFISHDLAVVRHMADEVGVMYLGRIVEIAASRALFER
                     PRHPYTRMLLAAIPDFSRRGRGDDAVRGEVPNPITPPSGCSFHPRCPLADARCRGEAP
                     VLRELDDGRRVACHAVEEGRDTIEAGAEICTEVS"
     gene            3654..4397
                     /locus_tag="PKN25_06550"
     CDS             3654..4397
                     /locus_tag="PKN25_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV90592.1"
                     /translation="MSGGTSEAARAEVRMEEAGGRTLAVVVGGGGGVGAAVVAALREA
                     GGHDEVLALGRRSSPALDLLDEASIAAAAAAVRARGVPTLVFDATGFLHGAGMEPEKS
                     WRELDAAHLAHAFAINAIGPALLMKHFLPLLPRDRRAVFATLSARVGSIGDNRLGGWY
                     AYRASKAALNQLVRTAAVELRRSRPQAICVALHPGTVDTGLSAPFAKSGLQVQAPAEA
                     AARLLAVIEGLGPGDSGEFFDHRGEPVPW"
     gene            4433..5080
                     /locus_tag="PKN25_06555"
     CDS             4433..5080
                     /locus_tag="PKN25_06555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV90593.1"
                     /translation="MNAQEQEHMWSTRYRSAGEDYVFGTTPNRFLAAQAALLAAGASA
                     LSVADGEGRNAVWLAEQGLAVTATEISPVALEKARKLAAGRHVEVDFVLADAVEWDYP
                     EAAFDFVVAIFIQFADPAQRARIFAGMARALKPGGHLILQGYTPQQLEYRTGGPSAVE
                     NLYTAALLREAFAGLDIVHVQEYEDVLEEGVGHKGRSALVGLVARRPPALGEGRR"
     gene            5077..5817
                     /locus_tag="PKN25_06560"
     CDS             5077..5817
                     /locus_tag="PKN25_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV90594.1"
                     /translation="MNAMRAVLTGHNRGLGEAVAAELLDRGIAVLALARRGNPALAAR
                     HGERLREVALDLADAAALAAWLGSGMLQGWLADADEALLINNAGTLQPMGPLALQDPA
                     AVARAVASNVGAPLALAAAFAAAEGPQARRVLHVSSGAARKPYPGWSVYCATKAALDH
                     HARAVQLDAVPGLRICALAPGVIDTGMQAEIRASTSERFPLRDRFAEMQASGALVAPA
                     ECAMHLVDFLLDEDFGREAVADLRDLGR"
     gene            5814..>6593
                     /locus_tag="PKN25_06565"
     CDS             5814..>6593
                     /locus_tag="PKN25_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5924 family protein"
                     /protein_id="HNV90595.1"
                     /translation="MKDESYPTTEALVASLQETESAPGTSGSRLARLKRRGLCVVVWL
                     RRHPGLIATFGFVSGVSSFFLVERHEGVARAMAVAMLAGWLLLVLERVLDRALERRFG
                     FGLPPALVRYLTQFTHQESLFFALPFFFASTSWNSGQAVFTGALGLMALVAILDPVYF
                     GRLATRRWLFLGYHTLTLFALLLVVFPLVLQVPALASYQLALALAVVLSFPTLTGAIS
                     VPRWWRGLLVLALLAALGAAGWLARLWVPPATLRLTQVAVTS"
     assembly_gap    6594..6693
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6744..7166)
                     /locus_tag="PKN25_06570"
     CDS             complement(6744..7166)
                     /locus_tag="PKN25_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV90596.1"
                     /translation="MTNRNISFIVKDQNPLMLRPTDTVQTACRSMCERSVGAVLVTDA
                     RAHLKGIFTGRDAVQVIANGGDPAKVQLSEVMTAKPDTIDPEHKAIDALHMMCDGGYR
                     HLPVVVDGKVVGIVSRSDFKGLELDQFEDEQSLWERIC"
     gene            7402..8982
                     /locus_tag="PKN25_06575"
     CDS             7402..8982
                     /locus_tag="PKN25_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HNV90597.1"
                     /translation="MEPQAGPLPATAESASPLRLRRVAIDTWRENVAYLHRDCAIYRA
                     EGFQALSKVEVRANGRRILATLNVVDDGCIVEPEELGVSIDAFFQLGVDDGHPAMIAQ
                     AEPPSSIPALHRKIAGERLAREDFRAIVRDIAEHRYSKIELTAFVVATNQGELDREEV
                     SFLTEAMAAVGDRIDWHERPVVDKHCIGGIPGNRTSMLVVPIVAAHGMLCPKTSSRAI
                     TSPAGTADTMEVLARVELPTEELSGIVREHRGCLAWGGTANLSPADDVLISVERPLSI
                     DSPGQMVASILSKKLAAGSTHLVLDIPVGPTAKVRSMAEAQKLRKLFEFVAGRMNLTI
                     DVVITDGRQPIGSGIGPVLEARDVMRVLENDPRAPYDLRQKALRLAGRMLEFDPDVRG
                     GDGFAIARDILDSGRALAKMDAIIRAQGARAFDHNAPQLARLSFEVCAEADGVVVAID
                     NHQLARIARLAGAPKMQGSGVDLLRKLGEPVGRGEPLYRVHADYPADLEFARQASVRA
                     SGYALGTADGVPHLFVEF"
     gene            9106..10005
                     /locus_tag="PKN25_06580"
     CDS             9106..10005
                     /locus_tag="PKN25_06580"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNV90598.1"
                     /translation="MSTEDTLLLHFDDEREEALRLARAAGLEAAEIERHRFPDDELRL
                     RLPAELPANAVLFRSLHRPNEKLLELLLVARSAGRGGVRHLILVAPYLAYMRQDKAFH
                     PGEVVSQRIVGGFLAGLFDGVITVDPHLHRISSLAEAIPVPHAVALSGAGLLADVAVA
                     RRDRPLLVGPDAESAQWVETAARRHGLDAIVCTKLRHGDREVDIALPEGAQVAGRAVV
                     LLDDVASSGHTLARAAERLRAAGAASVDVAVTHALFAGDALEVIRAAGVGEVWSTDCI
                     AHPSNAVHIAPMLAEALRAVLAD"
     gene            10199..11092
                     /locus_tag="PKN25_06585"
     CDS             10199..11092
                     /locus_tag="PKN25_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV90599.1"
                     /translation="MIFADLTGSTGLFESAGNVAATQIVTRCTHALGRHLACAGGRIV
                     KYLGDGVLVLFDDPVAAVQAASRLQDVLEELASAELRRAPIGVKTGIALGPIVEHDGD
                     CYGDPVNVAARLSDRAQTGETLIGEAVFASLPEGLRVACHSLDRISIKGKAEPMRVWR
                     VDFARTAETTLTTLLGFDDLVAGVRPVRRVDLERLDQRVELHADDPPLILGRGDGAGF
                     PVEDARVSRRHARLEWTAGQCVLTDFSSNGSWVRLRGNSAPVVLRRDSCTLYGEGEIG
                     LGAAPDDFTAPTLSFRVIDEG"
     gene            11165..12160
                     /locus_tag="PKN25_06590"
     CDS             11165..12160
                     /locus_tag="PKN25_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV90600.1"
                     /translation="MRILSILLLLLPFCATAQDWSARALGEIAVYPEFRAPASVVAGE
                     EARIAAEVGARIVAMPARTGVEVARGTELVRLDEAAFRIERDRARAQVALIDSRIGLA
                     RAQLDQARALAGRGFISADGLRIRETELGVLQRERDAARAAQAATELALARTVIRAPF
                     AGVVRERLAAVGDLAAPGTPLLSLAASAGAEVHARVPAAQIDGLLAAAPPVLVAGGAE
                     HALRIVRVSPVLDAAGQARDVILAAAPALPPGLAGELRWRSVVPHLPAAYLQQREGRL
                     GAWLVRGGEPAFVALPDAQAGRPVAVDWPADTRVVDAGRFALGLRAPTAAAGGSR"
     gene            12157..15234
                     /locus_tag="PKN25_06595"
     CDS             12157..15234
                     /locus_tag="PKN25_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV90601.1"
                     /translation="MNGLYRRLIDNHPLANIAFVMVLLGGLFSYLSMPRAQDPEINFN
                     WVSIVTALPGASAEDVERDLTGPLEDALKQVKDIRFSSSSSREGVSSILVRFEELPER
                     VFDKRVNDLRREIQNKASAELPPDANDPVVVEFTSSNGFPTAILVLHGAGGETLRRAG
                     FVMKKELEGLDGVDQVIAAGLEDPQLHVDFDSARVAAAGLSPLQLADSVAAWFRNTLA
                     GRVRVQDREWLVRMEGKTPDPEALAQAAVVTPAGRVALDEVASVARGHERPGQLVSYN
                     GAPALMLSITKDADANTLELVERLQDFAARRNPLLADQGVQLRLIDDQTHATRHAIGV
                     MESNAVFGLFAVLAMCWIFLGSRLSVLVGLGVPFALAGTFIAVDLIGSTLNLTVLLGV
                     VIALGMLVDDAVVIVEAIYYRLQRGDPPHEAVVAGVEEVGLPVLSSVLTTIAAFLPLM
                     LLPGILGKFMFVVPFVVTVGLLVSLVEAYWILPTHILALRPQPAQRPSRSQRLRERFT
                     LRIRIAYARALVAVMRRRRLTVAVLVLMMAGAGAAVATGLVRVQFFAFDSMRLFYVNV
                     DMPAGVVPQRTLAEAERAVKVLGETIGADELRAVAVYAGLKFTETEPVYGDQYAQVLV
                     SLLPRAAGGRDTGEIIDAVRPAIEALAGEAKLSFMEMKGGPPTAKPISVKMKSDDYPQ
                     LRAAADALLAEAAKLPGVRDLTDDDVPGRGEMRLALDREKLARAGVQPAEVARLLRLY
                     GEGEIVATTRDGGEKVEVVVRARQESLDDVTGLLQRPVPLADGRSVQLGELVSRQLLL
                     SKGYIRHYQLTRAITVEAELDRERLDTVEANDRLKAAWAQLQPRFPGVELDFSGELED
                     IQESLDAMGKLFALGVGLIYLILATQFRSYWQPLIILLTVPLAFTGVVLGLLVSGNPL
                     SLYTLYGVIALTGIAVNSAIVLIDAANERRAVGMSVQHAAIYAARRRVVPILITSTTT
                     IGGLLSLAIGLGGKSLMWGPVAASIVWGLGFSTVLTLFAVPLVYRMAMQRGGG"
     gene            15265..15840
                     /locus_tag="PKN25_06600"
     CDS             15265..15840
                     /locus_tag="PKN25_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HNV90602.1"
                     /translation="MDAFLVSTAIVALAEIGDKTQLLAFILAARFRRPWPIVLGILVA
                     TLANHAFAAAVGTWLTTLMGPHTLRWVLGLSFIAMAVWTLIPDKFDEDEARLPRLGVF
                     GATLVAFFLAEMGDKTQIATVALAAQYQALVAVVAGTTLGMMIANVPAVLLGDRIATR
                     MPVKLVHGIAAAIFLGLGIATLAGAGEGLGF"
     gene            15840..16544
                     /locus_tag="PKN25_06605"
     CDS             15840..16544
                     /locus_tag="PKN25_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNV90603.1"
                     /translation="MESSFRRGAREGMRDFLPLSVGLVPWAIVTGIAMRSIGLSPAES
                     MGMNLLVYAGTAQLGTLPLIGSGAPLWLIFLTALVLNLRFIIFSAALAPVFHDHRFMR
                     RLASSYLLVDGMFAVGADKLQRADDPHWRWGYYIVPSVYCWLLWQLCGAVGVFGAGAI
                     PRDWSLEFMTTIALTVMMIPMLRERPMWVAALVGGLGAVLLRELPLRLGLIAGIALGI
                     GAGFIAERALAGRAAR"
     gene            16541..16867
                     /locus_tag="PKN25_06610"
     CDS             16541..16867
                     /locus_tag="PKN25_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNV90604.1"
                     /translation="MSEGLWLWLTLLAIGSTTIMTRGSFILAGEKGRLPPALQRALRY
                     APAAALSALVMPDLLLDGGAFDPLNPKLAAGGVVILVALRSRNPWLPFILGMAMLLLL
                     RKGFGW"
     gene            complement(16968..18608)
                     /locus_tag="PKN25_06615"
     CDS             complement(16968..18608)
                     /locus_tag="PKN25_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNV90605.1"
                     /translation="MSQNAYQQGLDKNAANYVPLSPLSFIERTAMVYPTRTSVVHGTL
                     IYTWSQTYERCRRLASALVAHGVKKGDTVACMLPNVPSMFEAHFGVPMTGAVLNTLNT
                     RLDADAIAFMLQHGEAKVLITDPEFAGVVRAALELLPGDKPLVVDALDPEYPGTERIG
                     TVLYEDFLAGGNPDFQWKLPEDEWDAIALNYTSGTTGNPKGVVYHHRGAYLNAASNII
                     SWGMPQHSSYLWTLPMFHCNGWCFPWTMAANAGVNVCLRKVDPALIYELIRRHKVTHM
                     CGAPIVYGMLINAPEGLRAGIEHSVAGLIAGAAPPAAIIEGAEKIGFDITHVYGLTET
                     YGPASVCAKHPEWDALPIDRRAERNGRQGVRYHMQEAITVLDPVTMEPVPADGETMGE
                     IMFRGNLVMKGYLKNEKATQESFAGGWFHTGDLAVMHPDGYVKIKDRSKDVIISGGEN
                     ISSLEVEEVLYRHPAVLTAAVVAKPDEKWGEVPAAYIEVKEGAGVTADDIIAHCREHL
                     ARYKVPKFIEFCVLPKTSTGKIQKFALREMAKSASAIE"
     gene            complement(18756..19016)
                     /locus_tag="PKN25_06620"
     CDS             complement(18756..19016)
                     /locus_tag="PKN25_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HNV90606.1"
                     /translation="MNAAPSKPLPHERIRLIGTPVGRMVRIDDFDDEIDPLQREQLHA
                     YGLSAAHAVKVLQQQPMTVLLCEHVELAIEHAVSRHIWVQPA"
     gene            complement(19029..20993)
                     /gene="feoB"
                     /locus_tag="PKN25_06625"
     CDS             complement(19029..20993)
                     /gene="feoB"
                     /locus_tag="PKN25_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNV90607.1"
                     /translation="MDCATSPSSAAAAALRTLALVGHPNVGKSVLFHRLTGTYVTVSN
                     YPGTTVEVSRATPRFDREASLLDTPGVLALPSRSDDERATMRALLHEDMRTLIQVGDA
                     KNLRRTLNLTALLAELGVPMVMALNMTDEAKARGVTVDAEALSEALGVPVVATVATGG
                     EGVSALTEALQRAAVPAPLLRYDPVLERDIARVEQLILAQCPHPRLAARGLAILAIGR
                     DPEVEAWLAAQANSETVRAALAELTAPIGRDSLPARLARERGGAAEALARTVTQQAAG
                     KDRTLSMLLGRIAVHRLWGWPMLLGVLYLVYLFVGDFGASTLVGLLEEDFFGEVLNPA
                     VTEFVTSHISTPWLADLIVGEYGLWTMGMTYALALILPIVTTFFLAFGVLEDSGYFSR
                     LSVIANRMFAAIGLNGRAVLPMVLGLGCVTMATLTTRILHTPRERLITIYLMALAIPC
                     SAQLGVVLGLLGGISFGATVIWVLAIVGVLLFTGWLASKVVPGRRVPLVTELPPMRMP
                     VLGNVLKKTGGRLKWYLIEVIPLFLIGTLIMFGLDRSGALPWIIEAGEPLVVGWLGLP
                     PEASAAFVMGFLRRDFGASGLFALQSELSMIQAVVGMVTITLFVPCIASLMMIIKEQG
                     MKVALLMLAMIIPTAFLIGGLLNHGLRLIF"
     gene            21267..21878
                     /gene="rpsD"
                     /locus_tag="PKN25_06630"
     CDS             21267..21878
                     /gene="rpsD"
                     /locus_tag="PKN25_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNV90608.1"
                     /translation="MSRYTGPRLKVMRALGAELPGLSRKPLGERNHPPGQHGRRPRRK
                     SGFGVQLIEKQKLRFNYGLGEKQIQRLFREAKRDRGPTGDKLLELLERRLDNFVFRAG
                     FAPTAVAARQLVRHKHVLLNGRAVNIPSIRIRPGDRIQLTERARCIPVVVEALAEPAL
                     TRPEWLVFDKAAFVATMTRTPSPDEVPFPVDVQQVVEYYAVRL"
     gene            21875..22171
                     /locus_tag="PKN25_06635"
     CDS             21875..22171
                     /locus_tag="PKN25_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90609.1"
                     /translation="MSAHSSASRRRVAANAGPEYDTHPELALAAVLQLLSRFPARRSP
                     ALAQAIAGHFRVIGSDQRLSACVRDCAARLVAEWEAYAVLGDAGDGGHDPQALH"
     gene            22676..23359
                     /locus_tag="PKN25_06640"
     CDS             22676..23359
                     /locus_tag="PKN25_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90610.1"
                     /translation="MSHRKSGLRPIVLASLFALCSPPALAERPMAVDDAGTLERGGAK
                     LEFGWSRDDAVRGYEGAAGFGPIDNVEVEVGYGRAKDRDADPDAKIRAVGAAVKWVPL
                     QSESGLSAGLKYEYGRERVSGEDSSRVDALAGLATWAFEQGPRVHVNLGREWARDDED
                     LNFWGIGLDVPLGEQLDFVVETFGAEHSGPDRQVGLRYTIAEGVKISGAVGRGNDRNI
                     ANVGVAWEF"
BASE COUNT         3112 a         8030 c         8349 g         3769 t
ORIGIN      
        1 gtgcgcgccg agatgctcga ggtgctgcgc accgactaca tcaagttcgc ccgcgcgcgc
       61 gggctcaccg accgcacggt gcatttcggc cacgcgctgc gcaacaccct gctgccggtg
      121 atcaccatca ccgggctgca gatcggctcg atcatcgcct tcgccatcat caccgagacg
      181 gtattccagt ggccgggcgt gggcctgctc ttcatcaccg cggtgcagtt cgtcgacatc
      241 ccggtgatgg cggcctacct ggtgctgatc gcgctgatct tcgtcgtcat caacttgatc
      301 gtcgacctgc tctacttcgt cgtcgatccg cgcctgcgcg tgcagcgcac cgggcgcggg
      361 cagtgagcgc ccccggaacc ccggaaccca gcatgagcac cgaatccccc acgcccacca
      421 ccgctgccgc accgcgcggt gcgctcgcac gcctgctcga cagcgacctc ctgcacggct
      481 tcctccgctc gccgatcacc gtgctctcgg ccttgatcgt gctggcgatc ctgatcgccg
      541 cgttgctcgc gccggtgatc gcgccgcaga accccttcga tcccgccacg ctgaacctga
      601 tggacggctt cagccgcccc atgcaggcca acgcgttcac cggcaacgtg tattggctgg
      661 gcaccgacgc gcaggggcgc gacctcttct cggccatcct ctacggctcg cgggtgtcgc
      721 tgctggtcgg tttcgcggcg gtggccttcg ccgcggtgct cggcatcgcg ctcgggctga
      781 tcgccggcta tcgcggcggc tgggtggaca gcctgatcat gcgcatcgcc gacgtgcagc
      841 tgagcttccc ggcgatcctg gtggcgctgc tgatcttcgg cgtgttcaag ggcgtggtgc
      901 cgccggcgct gcacgacaag gcggcgatct acgtgctgat cctggcgatc ggcctgtcgg
      961 actgggtgca gtacgcgcgc acggtgcgca gctccacgct ggccgagcgc aacaaggaat
     1021 acgtgcaggc cgcgcgcgtg atcggcgtgc cggcgcccac catcctgctg cgccacatcc
     1081 tgcccaacgt gatggggccg gtgctggtga tcgccaccat cggcctcgcg ctggcgatca
     1141 tcctggagtc gacgctgtcc ttcctcggcg tgggcgtgcc gcccacccag cccagcctcg
     1201 gcaccctgat ccgcgtcggc caggactacc tgttctcggg cgagtggtgg atcgtgttct
     1261 tccccgggct gacgctgctg ctgctcgcgc tgtcggtgaa cctgctcggc gattggctgc
     1321 gcgacgcgct cgacccgagg ctgcgccgat gaacgtgccg cacatcgacc ggatgaacgc
     1381 cggcggcgac ctgccgctgc tgtcggtacg caacctgcgc gtggaatttc cccgccggcg
     1441 cgggacgctg gtggcgctcg acgacgtctc cttcgacatc gcgcgcggcg aggtgctcgg
     1501 cgtggtgggc gagtcgggtg cgggcaagtc gctcaccggc gcggccatca tcggcctgct
     1561 cgagcccccc gggcgtatcg ccggcggcga catcctgctc gatggcgagg ccatccacgc
     1621 gctgcgcggc gaggccatgc ggcgcctgcg cggacgccgc atcgcgatga tcttccagga
     1681 tccgctcacc agcctgaacc cgctatatac ggtgggcgag cagctggtcg agaccatgct
     1741 gacccacctc gacctgacgc ccgccgccgc gcgcgagcgc gcgctcgcgc tcctcgacga
     1801 ggtcggcatc ccggcgccgg cgcagcgcat cgaccattac ccccaccagt tctccggcgg
     1861 catgcgccag cgcgtggtga tcgcgctcgc cttgtgcgcc gagcccgagc tgatcatcgc
     1921 cgacgagccc accaccgcgc tcgacgtctc ggtgcaggcg cagatcatcg cgctgctgcg
     1981 tcgcctgtgc cgccagcacc gcaccgcggt gatgctgatc acccacgaca tgggcgtgat
     2041 cgccgagacc gccgaccgcg tcgcggtgat gtacgccggg cgggtggtgg agatcgggcc
     2101 ggtggccgag gtggtgcgcg ctccggcgca tccctacacc cgcggcctga tgggcgccat
     2161 cccggtgctc ggcgccgagg ttgagcgcct ggtgcagatc gacggcgcga tgccgcgcct
     2221 ggatgcgatc ccgtccggct gcgccttcca tccgcgctgc accgaggcca gcgcgcgctg
     2281 ccgcgtcgag cgcccggagt tgctgccggc cggcgccacg cgcgcggcgt gctggttgta
     2341 cgcgccggcg cgcgagaaca ccgacggagg cccgcagtga gcatcatcga gcgcgcgctc
     2401 gccaaggcga agcaggagga ccgcgccgcg gtcgagcagg ccgagggcct ggtgcatcgg
     2461 ccgcaggccg ggcaggacgc gcagggagcg gtcgcggagc ggacgggcga tgtggcggac
     2521 catcccgccg tggcggccgc ggtcgaagcc gagatcgagc ccgcagcgct tgcaccggcg
     2581 ccggctgcgg ctggcgcgcg ccgtgtgcgc gatgcgcgtg ccgcggcgcc gctgctgtac
     2641 gtcgaggacc tgcgctgcgc cttcgacgtc tcccggccct ggctcgagcg ggtgctcgcg
     2701 cgcgagccgc ggcgctggct gcgcgcggtc gatggggtct cgttcgcgat cgggcgcgga
     2761 tgcaccttcg cgctggtggg agagtcgggc tgcggcaaat ccacggtcgc gcgcctgatc
     2821 gtcggcctcc atgagccctc ggccggccgc atcgtcttcg acggccgcga cgtggccgag
     2881 gtgcgcggga gcgccgagcg ccagcagctg cgcagccgct tccagatgat cttccaggac
     2941 ccctacgcca gcctcaaccc gcgctggcgc gtggagcgca tcatcgccga gccgatccgc
     3001 gtgttcggac tggagcggga cgaggcggcg atccgtgcgc gcgtggccgc gctgctgggc
     3061 caggtcggcc tgtcgccggc cgacggcgcg aagtatccgc acgagttctc cggtgggcag
     3121 cgccagcgca tctgcatcgc gcgcgcgctc gccaaccggc ccgagttcct cgtctgcgac
     3181 gagccgacct cggcgctcga cgtgtcggtg caggcgcaga tcctcaacct gatgcgcgac
     3241 ctgcaggacg aactcgggct caccttcctg ttcatcagcc acgacctcgc cgtggtgcgc
     3301 cacatggccg acgaggtggg cgtgatgtac ctcggccgca tcgtcgagat cgccgccagc
     3361 cgcgcactgt tcgagcgccc gcgccacccc tacacgcgca tgctgctggc ggccatcccc
     3421 gacttttccc gccgtggtcg gggcgacgat gcggtgcgtg gcgaggtccc caacccgatc
     3481 accccgccat ccggctgcag cttccacccg cgctgcccgc tcgcagacgc gcgctgccgg
     3541 ggcgaggcgc cggtcctgcg cgagctcgac gacggccggc gcgtggcctg tcatgcggtg
     3601 gaggaggggc gcgacacgat cgaggcgggc gcggagatct gcacggaggt gtcatgagcg
     3661 gcggtacgag cgaggcggcg cgcgccgagg tgcgcatgga ggaagccggc ggcaggacgc
     3721 tcgccgtggt cgtgggcggc ggcggtgggg tgggcgcggc cgtggtcgcc gcgttgcgcg
     3781 aggcgggtgg ccacgacgag gtccttgccc tcgggcgccg gagcagcccc gcgctcgatc
     3841 tgctcgacga ggcctcgatc gccgccgcgg ccgccgcggt gcgtgcgcgc ggcgtgccga
     3901 ccctggtctt cgacgccacc ggtttcctcc atggcgcggg catggagccg gagaagagct
     3961 ggcgcgagct cgatgcggcc cacctggcgc acgcgttcgc gatcaacgcg atcggcccgg
     4021 ccctgctgat gaagcatttc ctgccgctgc tgccgcgcga ccgccgcgcg gtgttcgcca
     4081 cgctgtcggc gcgcgtgggc agcatcggtg acaatcggct cggcggctgg tacgcctacc
     4141 gcgcctcgaa ggcggcgctc aaccagctgg tgcgcaccgc cgcggtcgag ctgcgccgca
     4201 gccggccgca ggcgatctgc gtggcgctgc atccgggcac ggtcgacacc gggctgtcgg
     4261 cgcccttcgc caagtccggg ctgcaggtgc aggcccccgc cgaggcggcg gcgcgcctgc
     4321 tcgcggtgat cgagggcctc gggccgggcg actcgggcga attcttcgac caccgcggcg
     4381 aacccgtgcc gtggtgaccg ccgcgccgcc gcggcgcttt cagggaccag ggatgaacgc
     4441 acaggaacag gaacacatgt ggtccacccg ctatcgcagc gcgggcgagg actacgtctt
     4501 cggcaccacc cccaaccgct tcctcgccgc acaggcggcg ctgctcgcag ccggcgccag
     4561 cgcgctctcg gtggccgacg gcgaggggcg caacgcggtg tggctggccg agcaggggct
     4621 cgcggtgacc gccaccgaga tctcgccggt ggcgctggag aaggcgcgca agcttgccgc
     4681 cggccgccat gtcgaggtcg acttcgtgct cgccgacgcg gtcgagtggg actacccgga
     4741 ggccgccttc gacttcgtgg tggcgatctt catccagttc gccgacccgg cacagcgtgc
     4801 gcgcatcttt gccggcatgg cgcgcgcgtt gaagccgggc ggccacctga tcctgcaggg
     4861 ctacacgccg caacagctcg aataccgcac cggcggcccg tccgcggtgg agaacctgta
     4921 tacggccgca ctgctgcgcg aggccttcgc cgggctcgac atcgtgcatg tgcaggaata
     4981 cgaggacgtg ctggaggagg gtgtgggcca caaggggcgt tcggccctgg tcggcctggt
     5041 cgcgcgccgt ccgcccgcgc tgggggaggg gcgccgatga acgcgatgcg cgccgtgctc
     5101 accggacaca accgcggcct cggcgaggcg gtcgccgcgg agctgctcga tcgcggcatc
     5161 gcggtgctcg cgctcgcacg ccgcggcaat cccgcgctcg ccgcccgcca cggcgagcgc
     5221 ctgcgcgagg tcgcgctcga tctcgccgac gccgcggcgc tcgccgcctg gctcggctcg
     5281 ggcatgctgc aaggctggct cgccgacgcc gacgaggcgc tgctgatcaa caacgccggc
     5341 accctgcagc cgatgggacc gctcgcgctg caggacccgg cggcggtggc gcgtgcggtg
     5401 gcgagcaacg tcggcgcgcc gctggcgctc gccgccgcct tcgccgccgc cgaaggcccg
     5461 caggcgcgcc gcgtgctgca cgtctccagc ggtgcggcgc gcaagcccta cccgggctgg
     5521 agcgtgtatt gcgccaccaa ggccgcgctc gaccaccacg cccgcgcggt gcagctcgac
     5581 gccgtgccgg ggctgcgcat ctgcgcgctc gccccgggag tgatcgatac cggcatgcag
     5641 gccgagatcc gcgccagcac gtcggaacgc ttcccgctgc gcgaccgttt cgccgagatg
     5701 caggccagcg gcgctctggt cgccccggcc gagtgcgcga tgcatctggt cgacttcctg
     5761 ctcgacgagg acttcggtcg cgaggcggtg gccgacctgc gcgacctcgg acgatgaagg
     5821 acgagtccta cccgacgacc gaggccctgg tcgcctcgct tcaggaaacc gagtcggcgc
     5881 cggggacgag tgggagccgc ctcgcccgcc tgaagcggcg cggcctgtgc gtggtggtgt
     5941 ggctgcgccg ccatcccggg ctgatcgcca ccttcggctt cgtctccggg gtgtcgagct
     6001 tcttcctggt cgagcgccac gagggtgtgg cgcgcgcgat ggcggtggcg atgctggcgg
     6061 gctggctgct gctggtgctc gagcgcgtgc tcgaccgcgc gctcgagcgg cgcttcggtt
     6121 tcggcctgcc gccggcgctg gtgcgctacc tgacccagtt cacccaccag gagagcctgt
     6181 tcttcgcgct gcccttcttc ttcgcgagta cctcgtggaa cagcgggcag gcggtgttta
     6241 ccggcgccct cgggctgatg gcgctggtgg cgatcctcga cccggtctac ttcggccgcc
     6301 tggcgacacg gcgctggctc ttcctcggct accacacgct gaccctgttc gcgctgctgc
     6361 tggtggtgtt cccgctggtc ctgcaggtgc cggcgctggc gagctaccag ctcgcgctcg
     6421 cgctcgcggt ggtgctgtcc ttcccgacgc tgaccggggc gatcagcgtg ccgcgctggt
     6481 ggcgcgggct gctggtgctg gcgctgctcg ccgcgctcgg tgctgcgggc tggctggcgc
     6541 ggctgtgggt gccgccggcc acgctgcgcc tgacccaggt cgcggtgacc agcnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtggtgc gcgccggcct ggggacgtct
     6721 tgccggcggg tgtgccgtgg cggctagcag atgcgttccc agaggctctg ctcgtcctcg
     6781 aactggtcga gctccaggcc cttgaagtcg ctgcgcgaga cgatgccgac caccttgccg
     6841 tcgaccacca ccggcaggtg gcggtagccg ccgtcgcaca tcatgtgcag cgcgtcgatc
     6901 gccttgtgct cggggtcgat ggtgtcgggc ttcgccgtca tgacctcgga cagctgcacc
     6961 ttggccgggt cgccgccgtt ggcgatcacc tgcaccgcgt cgcggccggt gaagatgccc
     7021 ttgaggtgcg cgcgcgcgtc ggtcaccagc accgcgccca cgctgcgttc gcacatgctg
     7081 cggcaggccg tctgcacggt gtccgtggga cgcagcatca gcgggttctg gtccttgacg
     7141 atgaaggaga tgttgcggtt ggtcatggcg gcctcctctt gttctgcttg gtgtgatttc
     7201 tgtcacacca taggacgaat accggagcgc gatcttgata ttggttaagg ttcatgggta
     7261 tcgatcgaag gggcctggtg gcttcggcac ggtgtcgcgg tggcgagaga ctcgccgcgc
     7321 cgccgatcgc cgccgtcgtg gcgccgtctg cgtgtgaact gcgtcctgtt tcgtctgtcc
     7381 atgcgggtgt aggctgaatt tatggaacca caagccggac cgcttcccgc caccgccgag
     7441 tcggcctctc ccctgcgcct gcgcagggtg gcgatcgata cctggcgcga gaacgtcgcc
     7501 tacctgcacc gcgactgcgc gatctatcgc gccgagggtt tccaggccct ttccaaggtc
     7561 gaggtgcggg ccaacgggcg ccgcatcctc gccaccctca acgtcgtcga cgacggctgc
     7621 atcgtcgagc ccgaagaact cggggtgtcg atcgacgcct tcttccagct cggcgtcgac
     7681 gacggccatc cggcgatgat cgcgcaggcc gagccgccgt cctccatccc ggcgctgcat
     7741 cgcaagatcg ccggcgagcg gctcgcccgt gaggacttcc gcgccatcgt ccgcgacatc
     7801 gccgagcacc gctattccaa gatcgagctc accgccttcg tggtcgccac caaccagggc
     7861 gaactcgatc gcgaggaggt ctctttcctc accgaggcga tggcggcggt gggcgaccgc
     7921 atcgactggc acgagcgccc ggtggtggac aagcattgca tcggcggcat ccccggcaac
     7981 cgcacctcga tgctggtggt gccgatcgtc gccgcgcacg gcatgctgtg ccccaagacc
     8041 tcctcgcgcg cgatcacttc gcccgcgggg accgccgaca ccatggaggt gctcgcgcgc
     8101 gtcgagctgc cgaccgagga gctgtcgggc atcgtgcgcg agcatcgcgg ctgcctcgcc
     8161 tggggcggca ccgccaacct gtcgccggcc gacgacgtgc tgatctcggt cgagcgtccg
     8221 ctgtcgatcg actcgcccgg gcagatggtg gcctccatcc tgtccaagaa gctcgccgcg
     8281 ggttcgaccc acctggtgct cgacatcccg gtgggaccga cggccaaggt gcgctcgatg
     8341 gccgaggcgc agaagctgcg caagctcttc gagttcgtcg ccgggcgcat gaacctgacc
     8401 atcgacgtgg tcatcaccga tggccgccag ccgatcggca gcgggatcgg gccggtgctg
     8461 gaggcgcgcg atgtgatgcg cgtgctcgag aacgacccgc gcgcgcccta cgacctgcgc
     8521 cagaaggcct tgcgtctggc cgggcgcatg ctcgagttcg accccgacgt gcgcggcggc
     8581 gacggcttcg cgatcgcgcg cgacatcctc gactcgggtc gcgcgctggc gaagatggac
     8641 gcgatcatcc gcgcccaggg cgcgcgcgcc ttcgaccaca acgcgccgca gctcgcccgc
     8701 ctgagcttcg aggtgtgcgc cgaggccgac ggtgtggtgg tggcgatcga caaccaccag
     8761 ctcgcgcgca tcgcccgcct ggcgggggcg cccaagatgc agggctcggg cgtcgatctg
     8821 ctcaggaagc tcggcgagcc ggtgggcagg ggcgagcctc tctaccgcgt ccacgccgac
     8881 tatccggccg atctcgagtt cgcccgccag gccagcgtgc gcgccagcgg ctacgcgctg
     8941 gggacggcgg atggtgtgcc gcatctgttc gtggagttct gaactcgacg ttgccgaggc
     9001 gacgtgacag gcttccgggc ggaccgcgga ccgcgttccg ttttgcagcc ggggcgtgaa
     9061 tttctgcgcg gcgaccgccg ccgcacggcg tgtaatcttc cgccaatgag tactgaagac
     9121 accctgctgc tgcatttcga cgacgagcgc gaggaagcct tgcgcctcgc gcgtgcggcg
     9181 ggactcgagg cggcggagat cgagcgccac cgcttccccg acgacgagtt gcgcctgcgc
     9241 ctgccggccg aactgccggc caacgcggtg ctgttccgca gcctgcaccg tcccaacgag
     9301 aaactgctcg agctgctgct ggtggcgcgc agcgccgggc gcggcggcgt gcgccacctc
     9361 atcctggtcg cgccctatct ggcctacatg cgccaggaca aggcctttca tccgggcgag
     9421 gtggtgagcc agcgcatcgt cggcggattc ctggccgggt tgttcgacgg cgtcatcacg
     9481 gtcgatcccc acctgcatcg catctccagc ctcgccgagg cgattccggt gccgcatgcg
     9541 gtcgcgctct cgggcgccgg cctgctcgcc gacgtggcgg tcgcgcgccg cgaccgcccg
     9601 ctgctggtgg ggccggatgc cgagtcggcg cagtgggtcg agaccgcggc gcggcgccac
     9661 gggctcgatg ccatcgtgtg caccaagctc cgccacggcg accgcgaggt cgatatcgcg
     9721 ctgcccgagg gcgcgcaggt ggccgggcgt gcggtggtgt tgctcgacga cgtcgccagc
     9781 tccgggcaca ccctcgcgcg tgcggccgag cgcctgcgcg cggcgggtgc ggcctcggtc
     9841 gatgtcgcgg tcacccacgc gctgttcgcc ggcgacgcgc tcgaggtgat ccgtgccgcc
     9901 ggcgtgggcg aagtctggag caccgactgc atcgcccacc cgagcaatgc cgttcacatc
     9961 gcgccgatgc tggccgaggc cctgcgggcc gtcctcgccg actagcgtcc tgcagcgcgc
    10021 acccggcccc gcgcgtgcct tgccgcgggc gcatcgtcgc gtgcgcggcg atgacgatca
    10081 cggtccttaa tgctaggatt cttgcttgcg cactgcagcg agccccgctt ttcggggtcg
    10141 cgggcagtgc ccgcacatcc gtcggggcag gaattcagtg gagcaagcgg tcaggaccgt
    10201 gatcttcgcc gatctgacag gcagcacagg cctgttcgaa tcggctggca acgtggcggc
    10261 gacgcagatc gtcacccggt gcacgcacgc gctcggccgc cacctcgcct gtgccggcgg
    10321 gcgcatcgtc aagtatctcg gcgatggcgt gctggtgctg ttcgacgatc cggtcgccgc
    10381 ggtgcaggcg gcttcgcgcc tgcaggacgt gctcgaggag ctggccagcg ccgagctgcg
    10441 gcgggctccg atcggggtca agaccggcat cgcgctcggc cccatcgtcg agcatgatgg
    10501 cgactgctac ggcgatccgg tcaacgtcgc cgcgcgcctg agcgatcgcg cccagaccgg
    10561 cgagaccttg atcggcgagg cggtgttcgc cagcctcccc gaggggctgc gcgtggcctg
    10621 ccacagcctc gaccgcatca gcatcaaggg caaggccgag ccgatgcgcg tgtggcgggt
    10681 ggacttcgcc cgcaccgcgg agaccacgct caccacgctg ctcggtttcg acgacctcgt
    10741 cgcgggcgtg cgcccggtgc gccgggtcga tctggagcgc ctcgaccagc gcgtcgaact
    10801 gcacgccgac gatcccccgc tgattctcgg gcgtggcgac ggtgccggct tcccggtcga
    10861 ggacgcgcgc gtgtcgcgcc gccatgcgcg cctggaatgg accgccgggc agtgcgtgct
    10921 caccgatttc agcagcaacg gcagctgggt gcgcctgcgc ggcaactcgg cgcccgtggt
    10981 gctgcgccgc gacagctgca cgctctacgg cgagggggag atcggcctcg gcgccgcccc
    11041 cgacgatttc accgcaccca cgctctcctt ccgcgtcata gacgaaggct gagggcccat
    11101 gcacgacggg tctgcgggtg cgcccgccgt ctgcggcctc gcccatgcat tgcggtgtgt
    11161 tccgatgcga atcctgagta tcctcctgct cctgctgcct ttctgtgcga ccgcccagga
    11221 ctggagcgcg cgtgcgctcg gcgagatcgc ggtgtatccc gagttccgcg ccccggcgag
    11281 cgtggtcgcg ggcgaagagg cgcgcatcgc cgccgaagtc ggcgcgcgca tcgtcgcgat
    11341 gccggcgcgt accggcgtgg aggtcgcgcg cggcaccgag ctggtgcgcc tggacgaggc
    11401 cgccttccgc atcgagcgcg accgcgcgcg cgcccaggtg gcgctgatcg acagccgcat
    11461 cgggctggcg cgcgcccagc tcgaccaggc gcgcgcgctc gccggacgcg gcttcatcag
    11521 cgccgacggc ctgcgcatcc gcgagaccga actcggcgtg ctgcagcgcg agcgcgacgc
    11581 ggcacgcgcg gcccaggccg ccaccgagct cgcgctcgcc cgcaccgtga tccgcgcgcc
    11641 ttttgccggc gtggtgcgcg agcgcctcgc cgcggtgggc gatctcgccg cccccggcac
    11701 gccgctgctg agcctggcgg cgagcgccgg cgccgaggtg cacgcgcgcg tgcccgcagc
    11761 gcagatagac ggactgctcg ccgctgcgcc gccggtgctg gtcgcgggcg gcgcggaaca
    11821 tgcgctgcgc atcgtgcgcg tgtcgccggt gctcgatgcc gcgggccagg cgcgcgacgt
    11881 gatcctcgcc gccgcgcccg cgctgccgcc gggcctggcc ggcgagctgc gctggcgcag
    11941 cgtcgtgccg cacctgccgg ccgcctacct gcagcagcgc gagggccgcc tcggcgcctg
    12001 gctggtgcgc ggcggcgagc cggcgttcgt ggcgctcccc gacgcgcagg ccggccgccc
    12061 cgtcgcggtc gactggcccg cggacacccg ggtggtggac gcggggcgct tcgcgctcgg
    12121 actgcgggcg ccgaccgcgg cggccggggg ctcgcgttga acggcttgta ccggcggctg
    12181 atcgacaatc acccgctcgc caacatcgcc ttcgtgatgg tgctgctcgg cggcctgttc
    12241 agttatctct ccatgccgcg ggcgcaggac cccgagatca acttcaactg ggtcagcatc
    12301 gtcaccgcgc tgcccggcgc ctcggcggag gacgtcgagc gcgacctcac cgggccgctc
    12361 gaggacgcac tcaagcaggt caaggacatc cgcttcagct cctcgtccag ccgcgagggg
    12421 gtgtcgtcca tcctggtgcg cttcgaggag ctccccgagc gggtgttcga caagcgcgtg
    12481 aacgacctgc gccgcgagat ccagaacaag gcctcggcag agctgccgcc ggacgccaac
    12541 gaccccgtgg tggtcgagtt caccagctcg aacggcttcc cgaccgcgat cctggtgctc
    12601 catggcgccg gcggcgagac cctgcggcgc gcaggcttcg tgatgaagaa ggagctcgag
    12661 ggcctggacg gcgtggacca ggtgatcgcc gccggcctgg aagatccgca gctccacgtc
    12721 gatttcgatt ccgcccgggt ggcggcggcc gggttgtcgc cgctgcagct cgccgacagc
    12781 gtggcggcct ggttccgcaa caccctcgcc ggccgggtgc gggtgcagga ccgcgagtgg
    12841 ctggtgcgca tggagggcaa gacgcccgac cccgaggcgc tcgcccaggc tgcggtggtg
    12901 acgccggcgg gccgggtggc gctcgacgag gttgccagcg tggcgcgcgg ccacgagcgt
    12961 ccgggccagc tcgtgagcta caacggcgcg ccggcgctga tgctgtcgat caccaaggat
    13021 gccgacgcca acacgctcga gctggtcgag cgcctgcagg acttcgctgc ccggcgcaac
    13081 ccgctgctcg ccgaccaggg cgtgcagctg cgcctgatcg acgaccagac ccacgccacc
    13141 cgccacgcga tcggggtgat ggagtcgaac gcggtgttcg gcctgttcgc ggtgctggcg
    13201 atgtgctgga tcttcctcgg ctcgcgcctg tcggtgctgg tcgggctggg cgtgccgttc
    13261 gcgcttgccg gcaccttcat cgcggtcgac ctgatcggct ccacgctcaa cctcaccgtg
    13321 ctgctcgggg tcgtgatcgc gctcggcatg ctggtcgacg acgcggtggt gatcgtcgag
    13381 gcgatctact accgcctgca gcgcggcgat cctccgcacg aggcggtggt ggccggggtg
    13441 gaggaggtcg gcctgccggt gctgtcctcg gtgctgacca ccatcgccgc cttcctgccg
    13501 ctgatgctgc tgcccggcat cctcggcaag ttcatgttcg tggtgccctt cgtggtgacc
    13561 gtcggcttgc tggtcagcct ggtcgaggcc tactggatcc tgcccaccca catcctggcg
    13621 ctgcgcccgc agccggcgca gcggccctcg cggtcgcagc ggctgcgcga gcgcttcacc
    13681 ctgcgcatcc gcatcgccta tgcgcgtgcc ctggtggcgg tgatgcgccg ccgccgcctc
    13741 accgtcgcgg tcctggtgct gatgatggcg ggtgccggcg cggcggtggc gaccggcctg
    13801 gtgcgcgtgc agttcttcgc cttcgactcg atgcggctct tctatgtgaa cgtcgacatg
    13861 cctgccggtg tcgtgcccca gcgcacgctg gccgaggccg agcgcgcggt gaaggtgctg
    13921 ggcgagacga tcggcgccga cgagctgcgc gcggtcgcgg tgtatgcggg cctgaagttc
    13981 accgagaccg agccggtgta tggcgaccag tacgcccagg tgctggtctc gctgctgccg
    14041 cgcgcggccg gcgggcgcga taccggcgaa atcatcgacg cggtgcgtcc ggcgatcgag
    14101 gcgctcgccg gcgaagcgaa gctctccttc atggagatga agggcgggcc gccgacggcg
    14161 aagccgatct cggtgaagat gaagtccgac gactacccgc agctgcgcgc cgcggccgat
    14221 gcgctcctcg ccgaggcggc aaagctgccc ggggtgcgcg atctcaccga cgacgatgtg
    14281 cccgggcgcg gcgagatgcg cctcgcgctc gatcgcgaga agctggcgcg tgccggggtg
    14341 cagccggccg aggtggcgcg cttgctgcgt ttgtacggcg agggcgagat cgttgccacg
    14401 acgcgcgacg gcggcgagaa ggtggaggtg gtggtgcgcg cccgccagga gagcctggat
    14461 gacgtcaccg ggctgctgca gcgcccggtg ccgctggccg acgggcgcag cgtgcagctg
    14521 ggcgagctgg tgagccggca gctgctgctg tcgaagggct acatccgcca ctaccagctc
    14581 acgcgcgcga tcaccgtcga ggccgagctc gatcgcgagc gcctcgacac ggtcgaagcc
    14641 aacgaccgcc tgaaggccgc gtgggcgcag ctgcagccgc gcttcccggg cgtggagctg
    14701 gatttctccg gcgaactgga ggacatccag gagagtctgg atgcgatggg caagctgttc
    14761 gcgctcggcg tcgggctgat ctacctgatc ctcgccaccc agttccgcag ctactggcag
    14821 ccgctgatca tcctgctgac cgtgccgctc gccttcaccg gggtggtgct cggactgctg
    14881 gtgtcgggca atccgctgtc gctatacacg ctgtatgggg tgatcgcgct caccggcatc
    14941 gcggtgaact cggcgatcgt gctcatcgac gccgccaacg agcgccgcgc cgtcggcatg
    15001 agcgtgcagc acgccgcgat ctacgccgcg cgccggcgcg tggtgccgat cctgatcacc
    15061 tccaccacca ccatcggcgg gctgctttcg ctggcgatcg gcctgggcgg caagtcgctg
    15121 atgtggggac cggtggcggc gagcatcgtg tgggggctgg gtttttccac ggtgctgact
    15181 ttgttcgcgg tgccgctggt gtatcggatg gcgatgcagc gcggcggcgg ctagaatcgc
    15241 gctcccgccg ccacctgaat tcccatggac gcctttctcg tctctaccgc catcgtggcc
    15301 ctcgccgaga tcggcgacaa gacgcagctg ctcgccttca tccttgccgc acgcttccgc
    15361 cggccgtggc cgatcgtgct cggcatcctg gttgccacgc tggccaacca cgccttcgcc
    15421 gccgcggtcg gcacctggct gaccacgctg atggggccgc acaccctgcg ctgggtgctc
    15481 ggcctgtcct tcatcgcgat ggcggtgtgg acgctgatcc ccgacaagtt cgacgaggac
    15541 gaggccaggc tgccgcgcct gggcgtgttc ggcgccacgc tggtggcctt cttcctcgcc
    15601 gagatgggcg acaagacgca gatcgccacg gtggcgctgg cggcgcagta ccaggccctg
    15661 gtcgcggtgg tggccggcac cacgctcggc atgatgatcg ccaacgtgcc cgcggtgctg
    15721 ctcggcgacc gcatcgccac gcgcatgccg gtgaagctgg tgcacggcat cgccgcggcg
    15781 atcttcctcg gcctgggcat cgcgaccctg gccggtgcgg gcgagggact ggggttctga
    15841 tggaatcctc gttccgccgc ggtgcccgcg agggcatgcg cgacttcctg ccgctctcgg
    15901 tcggcctggt gccctgggcg atcgtgaccg gcatcgccat gcgctcgatc ggcctgtccc
    15961 cggccgagtc catgggcatg aacctgctgg tctatgccgg caccgcccag ctcggcacgc
    16021 tgccgctgat cggcagcggt gcacccttat ggctgatctt tctcactgcg ctggtgctca
    16081 acctgcgctt catcatcttc agtgccgcgc tggcgccggt cttccatgat caccgcttca
    16141 tgcgccggct ggcgtcgagc tatctgctgg tggacggcat gttcgcagtc ggcgcggaca
    16201 agctgcaaag ggccgacgat ccgcactggc gatgggggta ctacatcgtg ccctcggtgt
    16261 attgctggct gctttggcag ctctgcggtg cggtcggcgt gttcggcgca ggggcgattc
    16321 cgcgcgactg gtcgctggag ttcatgacca cgatcgcgct gacggtgatg atgatcccca
    16381 tgctgcgcga gcgaccgatg tgggtggctg cgctggtggg cgggctgggt gcggtgctgc
    16441 tgcgcgagct gccgctgcgc ctggggctga ttgccggcat cgcgctcggc atcggcgcgg
    16501 gcttcattgc cgagcgtgcg ctggcaggga gggccgcgcg atgagcgagg ggttgtggtt
    16561 atggctgacg ctgctggcca tcggctcgac gacgatcatg acgcgtggca gcttcatcct
    16621 cgccggcgag aaggggcgtc tgccgccagc cttgcagcgt gccttgcgct acgcgccggc
    16681 ggcggcgctg tcggcgctgg tgatgcccga cctgctgctc gatggcggtg cgttcgatcc
    16741 gctcaatccc aagctcgcgg cggggggagt cgtgatcctg gtcgcgctgc gctcgcgcaa
    16801 tccgtggctg cccttcatcc tcggcatggc gatgctgctg ttgctgcgca agggattcgg
    16861 ctggtagtac gtgtggaagg aaaaatgccg cgtccgtccg gtgacggaac gcggcatcgg
    16921 cgcgcggcga gtgcggtcgg ccccgacggg ccgaccgcgt gacacgatca ctcgatcgcg
    16981 gaggccgact tcgccatctc gcgcagggcg aacttctgga tcttgccggt cgaggtcttg
    17041 ggcagcacgc agaactcgat gaacttgggc accttgtagc gcgccaggtg ttcgcggcag
    17101 tgcgcgatga tgtcgtcggc ggtcacgccg gcgccttcct tgacctcgat gtaggccgcc
    17161 ggcacctcgc cccacttctc gtcgggcttg gcgacgaccg ccgcggtcag caccgccggg
    17221 tggcggtaca gcacctcttc gacctcgagc gaggagatgt tctcgccgcc cgagatgatc
    17281 acgtccttgg agcggtcctt gatcttgacg tagccgtccg ggtgcatgac cgcgaggtcg
    17341 ccggtgtgga accagccgcc ggcgaaggac tcctgggtgg ccttctcgtt cttgaggtag
    17401 cccttcatga cgaggttgcc gcggaacatg atctcgccca tggtctcgcc gtcggcgggc
    17461 accggctcca tggtcaccgg gtcgagcacg gtgatcgctt cctgcatgtg gtagcgcacg
    17521 ccctggcggc cgttgcgctc ggcgcggcgg tcgatcggca gcgcatccca ctcggggtgc
    17581 ttggcgcaca ccgaggccgg gccgtaggtt tcggtcaggc catacacgtg ggtgatgtcg
    17641 aagccgatct tctcggcgcc ctcgatgatc gccgccgggg gcgcggcgcc ggcgatgagg
    17701 ccggcgacgc tgtgctcgat gccggcgcgc aggccctcgg gggcgttgat cagcatgccg
    17761 tagacgatcg gcgcgccgca catgtgggtg accttgtggc ggcggatcag ctcgtagatc
    17821 agcgccgggt cgaccttgcg caggcagacg ttgacgccgg cgttggccgc catggtccac
    17881 gggaagcacc agccgttgca gtggaacatc ggcagcgtcc acaggtagga cgagtgctgc
    17941 ggcatgcccc agctgatgat gttggaggcg gcgttgagat acgcgccgcg gtggtggtag
    18001 accacgccct tcgggttgcc ggtggtgccg gaggtgtagt tgagcgcgat cgcgtcccac
    18061 tcgtcctcgg gcagcttcca ctggaagtcg gggttgccgc cggcgaggaa gtcctcgtag
    18121 agcacggtgc cgatgcgctc ggtgcccgga tactcggggt cgagggcgtc gaccacaagg
    18181 ggcttgtctc ccggcagcag ctcgagcgcg gcgcggacca cgccggcgaa ctcggggtcg
    18241 gtgatcagca ccttggcttc gccgtgctgc agcatgaagg cgatggcgtc ggcgtcgagc
    18301 cgggtgttga gggtgttgag gaccgcgccg gtcatcggca ccccgaagtg ggcttcgaac
    18361 atcgacggca cgttgggcag catgcacgcg accgtgtcgc ccttcttcac gccgtgcgcg
    18421 accaaggcgc tcgcgaggcg gcggcagcgc tcgtaggtct ggctccaggt gtagatgagc
    18481 gtgccatgga cgacgctggt gcgcgtcgga tagaccatgg cggtgcgctc gatgaagctc
    18541 agcggggaca gcggcacgta gttggccgcg ttcttgtcga ggccttgctg atatgcgttt
    18601 tgcgacatcc ttttcctctc ccgttcattg tcaaacaaac tggtcgggcc ggcgtcccac
    18661 gcggggttcg gtccataccg tatagtatgg aggatggcgt cggtcttgtc tgcagttctc
    18721 ccgcgcgcaa cgtttgcgaa ggcaggttgg agcgctcagg cgggctgcac ccagatatgg
    18781 cgcgacaccg cgtgctcgat cgcgagctcg acgtgctcgc acaatagcac cgtcatcggt
    18841 tgctgttgca gcaccttcac cgcgtgcgcg gcggagagcc cgtaggcgtg gagctgctca
    18901 cgctgcagcg gatcgatctc gtcgtcgaag tcgtcgatgc gcaccatgcg cccgaccggg
    18961 gtgccgatca ggcggatgcg ctcgtggggc aggggtttgg agggggcggc gttcatggcg
    19021 cggattcctc agaagatcag gcgcaggccg tggttgagca ggccgccgat caggaaggcg
    19081 gtggggatga tcatcgccag catcagcagg gcgaccttca tcccctgctc cttgatgatc
    19141 atcatcaggc tggcgatgca gggcacgaag agcgtgatcg tgaccatgcc caccacggcc
    19201 tggatcatcg acagctcgct ctgcagcgcg aacaggccgc tggcgccgaa gtcgcggcgc
    19261 aggaagccca tcacgaaggc ggcgctcgcc tccggcggca ggccgagcca gcccaccacc
    19321 agcggctcgc cggcctcgat gatccacggc agcgcgccgc tgcggtcgag cccgaacatg
    19381 atcagggtgc cgatcaggaa cagcgggatc acctcgatca ggtaccactt cagccttccg
    19441 ccggtcttct tgagcacgtt gccgagcacc ggcatgcgca tcggcggtag ctcggtgacg
    19501 agcggcacgc ggcggccggg cacgaccttg ctcgccagcc agccggtgaa cagcagcacg
    19561 ccgacgatcg ccagcaccca gatcacggtg gcaccgaagg agatgccgcc gagcaggccg
    19621 agcaccacgc cgagctgcgc agagcacggg atcgccagcg ccatcaggta gatggtgatc
    19681 aggcgctcgc gcggggtgtg caggatgcgg gtggtgagcg tggccatggt cacgcagccc
    19741 aggccgagca ccatcggcag caccgcgcgg ccgttcagac cgatcgccgc gaacatgcgg
    19801 ttggcgatca ccgacaggcg cgagaagtag cccgagtcct cgagcacgcc gaaggcgagg
    19861 aagaaggtgg tgacgatggg caggatcagc gcgagcgcgt aggtcatgcc catggtccac
    19921 aggccgtatt cgcccacgat caggtcggcc agccagggcg tggagatgtg gctcgtgacg
    19981 aactcggtga ccgcggggtt gaggacctcg ccgaagaagt cttcttccag cagtccgacc
    20041 agggtgctgg cgccgaagtc gccgacgaac agatacacca gatacagcac gccgagcagc
    20101 atcggccagc cccacagccg gtgcaccgcg atgcggccga gcagcatcga cagcgtgcgg
    20161 tccttgccgg cagcctgctg cgtgacggtg cgcgcgagcg cctcggcggc gccgccgcgc
    20221 tcgcgcgcga gccgcgccgg gagcgagtcg cggccgatgg gcgcggtgag ctcggcgagc
    20281 gcggcgcgca cggtctcgct gttcgcttgc gccgcgagcc aggcctcgac ctcggggtcg
    20341 cggccgatgg cgaggatggc cagcccgcgt gcggccaggc gcggatgtgg gcattgcgcg
    20401 aggatgagct gctcgacgcg ggcgatgtcg cgctcgagca ccgggtcgta gcgcagcagt
    20461 ggtgccggca ccgccgcacg ctgcagcgct tcggtgagcg cggaaactcc ttccccgccg
    20521 gtcgccaccg tcgccaccac cggcacgccg agtgcctccg acaaggcctc ggcatccact
    20581 gtgaccccgc gcgccttggc ctcgtcggtc atgttgagcg ccatcaccat cggcaccccg
    20641 agttcggcca gcagcgcggt gaggttgagc gtgcggcgca ggttcttggc gtcgccgacc
    20701 tggatcagcg tgcgcatgtc ctcatggagc agcgcgcgca tggtggcgcg ctcgtcgtcg
    20761 ctgcgcgatg gcagggcgag cacgcccggg gtgtcgagca gcgaggcctc gcggtcgaag
    20821 cgcggtgtgg cgcgcgagac ctcgaccgtg gtgccggggt aattggacac cgtgacgtag
    20881 gtgccggtga ggcggtggaa cagcaccgac ttgccgacgt tggggtggcc gacgagggcc
    20941 agggtgcgca gcgcggcggc agcggcggag gagggcgaag tggcgcagtc catgaactcg
    21001 ggtgtccgga gggcggagta agcctagaac gataatgggt cgcattatga cttttactga
    21061 tctagctcaa aggtcgtgcg cccggtgtgt attcgatgcg gtccggaccg caggacagag
    21121 aggcttgaca gtgattcgga ttttaacgat attgcgaatc attcgcaaag acgatattca
    21181 accgaccctg tctcgacttc gaggctgtcc tgctggcgct cttcgtgcgt cggcgcagca
    21241 agaatccctg aatgaaggag aacccattgt cccgatacac cggcccccgc ctgaaagtga
    21301 tgcgtgccct cggcgccgag ctgcccggcc tgtcgcgcaa gccgctgggt gagcgcaacc
    21361 atccccctgg ccagcacggc cggcgcccga ggcgcaagtc cggcttcggc gtgcagctga
    21421 tcgagaagca gaagctgcgc ttcaactatg gtctcggcga gaagcagatc cagcgcctgt
    21481 tccgcgaggc caagcgcgac cgcggcccga ccggcgacaa gctgctggaa ttgctcgagc
    21541 gtcgcctcga caacttcgtg ttccgcgccg gcttcgcgcc caccgcggtg gcggcgcggc
    21601 agctcgtgcg ccacaagcac gtactgctca acggacgggc ggtgaacatc ccctccatcc
    21661 gcatccggcc gggcgaccgc atccagctca cggaacgtgc gcggtgcatc ccggtggtgg
    21721 tcgaggcgct cgccgagcct gcgctgaccc gccccgaatg gctcgtcttc gacaaggcgg
    21781 cgttcgtggc gacgatgacg cgcaccccgt cgcccgacga ggtgcccttc ccggtcgacg
    21841 tccagcaggt cgtcgagtac tacgcggtgc ggctgtgagc gcgcattcat ccgccagccg
    21901 gcgaagggtt gccgcgaacg cggggccgga gtacgacacc catcccgagc tcgcgctggc
    21961 ggcggtgctg cagctgctgt cgcgcttccc ggcgcggcgc tcgcccgcgc tggcgcaggc
    22021 gatcgccggt cacttccggg tgatcggcag cgatcagcgc ctttccgctt gtgtgcgcga
    22081 ctgcgccgcc cggctggtgg cggagtggga ggcctatgca gtgctgggcg atgcgggcga
    22141 tggcggccac gatccgcagg cactgcactg agcagtccgg ttcgatggtg tccgtctttt
    22201 ccattccacg aggagaagtc catgagcaag aaatgctgca ggaaggacaa gccctgcaag
    22261 ggatgtccgc ggcgcgcaaa gtaagccgga atcccgcgcg ctgttgatga ttatcaaatg
    22321 gtaatgattg ttgttgcggt taatattttt caacccatcc ccaggcccgg cgaccgcgat
    22381 catcggctgc attgaggcgg gggccgaata ttccgggaag ctcgatcgcg atgcggtgct
    22441 cgaacatggc cgcaccgccc tttccgtgtc tggccaggtg ctcgagtaag aagcccctcc
    22501 cggcgcatgc gggcacgtcc gaggcacgag tgtcgggcgt ccctgccgca tcgacggcgt
    22561 gggtcttgtc cagcgatcac ctcgtagccc cggtcgttcg gagttgcgca tcccttttcg
    22621 ggccgctcgt gtgggcggcc cgtctttgtc cacctctccc tgcaaaggaa tcgtcatgtc
    22681 tcatcgcaag tccgggctgc gcccgatcgt gctggcctcg ctgttcgcct tgtgttcccc
    22741 tccggctctc gccgaacgcc cgatggcggt cgatgatgcc ggcacgctcg agcgcggcgg
    22801 cgccaagctc gagttcggct ggagccgcga cgacgcggtg cgcggctacg agggcgcggc
    22861 cggtttcggt ccgatcgaca atgtcgaagt ggaggtgggc tatggccgcg cgaaggatcg
    22921 tgacgccgat cccgacgcga agatccgcgc cgtcggcgcg gcggtcaaat gggtgccttt
    22981 gcagtccgag agcgggcttt cggctggcct gaaatacgag tatgggcgcg agcgcgtcag
    23041 cggcgaggac tcctcgcgtg tcgacgcgct cgccggcctg gcgacctggg cgttcgagca
    23101 gggcccgcgg gtgcatgtga acctcggccg cgagtgggcg cgcgacgacg aggacctcaa
    23161 cttctggggc atcggcctcg acgtgccgct cggcgagcag ctcgacttcg tcgtcgagac
    23221 cttcggtgcg gagcattccg gcccggatcg ccaggtcggt ctgcgctaca cgatcgccga
    23281 gggcgtgaag atctctggcg cggtcggtcg cggcaacgat cgcaacatcg ccaacgtggg
    23341 cgtggcctgg gagttctgag
//
LOCUS       DAOQBS010000041        23260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1434_length_23260_cov_15.652877, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000041 DAOQBS010000000
VERSION     DAOQBS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 23260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23260
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1434_length_23260_cov_15.652877"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PKN25_06645"
     CDS             <1..778
                     /locus_tag="PKN25_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNV90611.1"
                     /translation="VNFGLTADRTEITTYNASPLLYKDFCKENGLDNPSGVGDCTVDS
                     LLLTVGWSRDGRDSFTYPRSGVYQRVYGEVTVPVGDLRYGKLGYQYQHWFPIGTDYAL
                     MLNADVGYAKGFGGKDVPFYKNYYVGGIGSVRGFDQGSVGPKYKDTDGDISSTGGTRK
                     AVANAEFFFPMPGAGKDRSFRISAFLDAGYVWGRDEEPLGGGTFVDQNIRFSDLRYST
                     GLAFSWSSPIGPLKFSFGHPLKKEENDEIQRFQFQLGSVF"
     gene            813..1313
                     /locus_tag="PKN25_06650"
     CDS             813..1313
                     /locus_tag="PKN25_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNV90612.1"
                     /translation="MKVSTLSLVALAVLGLSQTAFAETKIGFVNSDRVMREAAPAVRA
                     QQRLEKEFEKRDQELQRIARDLKSMQEDLERNGPTMGDSDRRNKERAFNELNRDFQRK
                     QREFREDLNQRRNEELASVLDKANRSVKQIAEQENYDIIFQEAVYANPRIDITDKVIK
                     TLSDAK"
     gene            1327..2361
                     /gene="lpxD"
                     /locus_tag="PKN25_06655"
     CDS             1327..2361
                     /gene="lpxD"
                     /locus_tag="PKN25_06655"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNV90613.1"
                     /translation="MFRLHELVDRLGGEIAGDGDVAVSRVGTLDKAGEGDLAFLANRK
                     YLSQVGECAASALIVTPDLREALAGRNLILTADPYLYFARVAQLFNPPPAVRPGIHAL
                     AAVASTLPASVQVGPGASIEEDVVLGEDVVIGPNCHVGRGTRIGRGTRLYANVSIYHD
                     CVIGEDCILHSGVVIGADGFGFAREKSGAWVKIPQTGRVVLGNDVEIGANTTVDRGAL
                     DDTVIGDGVKLDNLIQIAHNVRVGEHTIMAGCAGVAGSARIGARCMIGGQAGISGHLS
                     IADDVVVSAWTLVAKSIAKPGVYTGNLPLQTHGDWVKNFSHLRHLDALARRVRQLEQN
                     SDGPDDRSQG"
     gene            2368..2802
                     /gene="fabZ"
                     /locus_tag="PKN25_06660"
     CDS             2368..2802
                     /gene="fabZ"
                     /locus_tag="PKN25_06660"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNV90614.1"
                     /translation="MDIKEILQYLPHRYPFLLVDRMLEIEPGVRARGLKNVTMNEPFF
                     PGHFPHHPVMPGVLIIEAMAQAAALLSFKSTGVKPDDNSVVYFAGIDNARFKRPVVPG
                     DQLIFDVEITHAKRNIYKYKARASVDGELAAEAELMCAIKTL"
     gene            2799..3569
                     /gene="lpxA"
                     /locus_tag="PKN25_06665"
     CDS             2799..3569
                     /gene="lpxA"
                     /locus_tag="PKN25_06665"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNV90615.1"
                     /translation="MIHPTAIVHPGAKLGANVSVGAYSLIGENVEIGDGTRIGPHVVV
                     EGHTRIGRDNEIFQFCSIGASPQDKKYDAEPTRLEIGDRNTIREFCSFNVGTSQDAHV
                     TRVGNDNWIMAYVHIAHDCQVGDHTIFANNATLAGHVHVGDWAILGGFTGVHQFCRVG
                     AHSFCGVGTVLLQDLPPFVTVAGNPAKPHGINSEGLKRRGYSAEGIAAIKRAYRALYR
                     SGLTLDEARQRVAEIAAGQAEAAPFAAFIADSGRGIVR"
     gene            3610..4767
                     /gene="lpxB"
                     /locus_tag="PKN25_06670"
     CDS             3610..4767
                     /gene="lpxB"
                     /locus_tag="PKN25_06670"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNV90616.1"
                     /translation="MKIRIAMVAGETSGDLLASHLIRALRRHLPDAEFFGIGGPKMQA
                     EGFDVRWPCELLAVHGYVDALKRYRELSGIRRALLAQIRAERPDAFIGVDAPDFNLWL
                     EGKVRDAGIPAIHFVGPSIWAWRGGRIKRIARSVSHMLCLFPFEPELYERAGVPVSYV
                     GHPLADEFPLEPDRAAARERLGIPLERGVVAMLPGSRQSEVRNLADIFIGTAKTLHER
                     DPERLFLVPLATRETRQLFEEALHRNDAGGLPIRMLFGHAVEAMTAADVVLVASGTAS
                     LEAALLKRPMVITYRIGKWQYHLMKRMAYLPWVGLPNILCGETVVPELLQDEADPEHL
                     AAAIDDWFADDARRAAVAARFSDLHRTLRQDTARRAAEAILPYLDKAGKAS"
     gene            4764..5375
                     /gene="rnhB"
                     /locus_tag="PKN25_06675"
     CDS             4764..5375
                     /gene="rnhB"
                     /locus_tag="PKN25_06675"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNV90617.1"
                     /translation="MTGDLFAPDLSASGLVCGVDEAGRGPLCGPVVAAAVILDPARPI
                     EGLADSKKLSEAARERLAPLIRARALAWAVAEASVEEIDRLNILHATMLAMQRAVMAL
                     GIAPAEVRIDGNRCPKLPYPARAIVKGDATEPSISAASILAKTARDAAMRELDAQWPQ
                     YGLAAHKGYPTAAHLAALAAHGVQAFHRRSFAPVKKLLERRDE"
     gene            5368..6201
                     /locus_tag="PKN25_06680"
     CDS             5368..6201
                     /locus_tag="PKN25_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNV90618.1"
                     /translation="MSEPQLKALASRDNAFVKRLHALAHSARDRRKLDETVLDGAHLV
                     ESALAAGVALRALVVSESGLLREEHRRLLAAAPAEVPRYRLGDALFAHVSPVDSPAGL
                     LATIAMPASTPSAPGDASLIVVDGVQDPGNLGSLLRTAAAAGIRHALLGEGCAQAWSP
                     RALRAGMGAQFVVRIEEGCELLARLESYPGLCLATALREDAHPLYELDLRGPVAWLFG
                     AEGQGLSPALLARADGCVIIPMAAGIESLNVAAAAAVCLFEQARQLRATGTIRGVNFP
                     S"
     gene            6313..6825
                     /locus_tag="PKN25_06685"
     CDS             6313..6825
                     /locus_tag="PKN25_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90619.1"
                     /translation="MEKYAKTPKGQDEIAHRTHGLGLRARRLLILVDGQRSVEDLCAL
                     TGDAKIAAALGELADAGFIAPSAGMTDPPEASIREGQPAAGAAPVARAHTGHTASLPL
                     ARDFMINTLRTFHGPYGKLGLVKRVHATRSSSELRALIAEWKESIDESRLGRKRADEL
                     LALLEEVLPG"
     gene            6898..7683
                     /gene="yaaA"
                     /locus_tag="PKN25_06690"
     CDS             6898..7683
                     /gene="yaaA"
                     /locus_tag="PKN25_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNV90620.1"
                     /translation="MILVISPAKALDYETPITVSNYTRPDFLDDSAALIEVLREKSPA
                     EIAELMSLSDPLSTLNVARYASWSRPFSPDNARQALLAFNGDVYEGLDARSLTADELG
                     WAQDHLRILSGLYGVLRPLDLMQAYRLEMGTRLANPRGKNLYEFWGERITDALNGLLA
                     REEDAGRERVLLNLASDEYFKSVKPKKLKGRIVTPVFEDWKGGRYKIVSFYAKRARGL
                     MSRYVITNRIDEVDALQGFDAEGYAFAADASDADTLVFRRREE"
     gene            7689..8825
                     /locus_tag="PKN25_06695"
     CDS             7689..8825
                     /locus_tag="PKN25_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HNV90621.1"
                     /translation="MKISSTFDSGAIEVVRLDDPADIRLRLRKDNAADFHQWFHFRLM
                     GAAGKPVRMVFENAAGAAYPDGWRDYRCVASYDRQNWFRIAGTRYEDGQLIVEHTPER
                     DSLYYAYFEPYSHERHLDLLARVELSPYARVRNLGSTVDGRDLDVVTVGRDAPGRVPV
                     WIIARQHPGETMAEWFVEGLLERLLDNADPVARKVREHAVLHIVPNMNPDGAVRGNLR
                     TNAAGRNLNREWRAPDPVASPEVFLVRAEMERTGCALFLDIHGDESLPYVFFSTAEEV
                     PGFTAAAAAAQARFVETLLMVSPDFQTQEGYKPGRFGEELLTLASKWVANQFGCVSLT
                     LEMPFKDNIILPDGHVGWNGARSKRLGAAMLDPILRHLREGGEG"
     gene            complement(8922..10247)
                     /gene="hpnE"
                     /locus_tag="PKN25_06700"
     CDS             complement(8922..10247)
                     /gene="hpnE"
                     /locus_tag="PKN25_06700"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HNV90622.1"
                     /translation="MSVPVVAIIGAGYAGLACGVELARAGVHVTVFERSHTMGGRARV
                     VHKDHHWRVDNGQHILIGAYSELTRLLRLTGVSPKQLAHLPLTLHVPGRLHLKAASLP
                     APFHLAVGLLRAQGLHWADRLAMLRLMRWLKRRGFRLPDPGMTVDALLAETRQTPTLC
                     QLIWEPLCVAALNTPAAAASAQVFANVLRDSLGAGAAASELLLPRTDLSELFPVPAAR
                     YIGVRRGRLRTGNAIEAVERVAGGYRLDGDPGSHPWPHVVVATAPYHAGALLASVGGC
                     ERLVAQIDALEHEPIVSVYLALGRGQQLPEPMIGLASGSTAGPAQWVFDRGQLGGPEG
                     LFSCVISAHGPHEALARDELVLAVHAQLERQLGRRLPAPEWSQVIVEKRATFACRPGV
                     FRPGIRTPLPGLWLAGDYLDSDYPATLESAVRSGVACATAILAEIGHKA"
     gene            complement(10315..11148)
                     /gene="hpnD"
                     /locus_tag="PKN25_06705"
     CDS             complement(10315..11148)
                     /gene="hpnD"
                     /locus_tag="PKN25_06705"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HNV90623.1"
                     /translation="MNPHDYCQDKAAKSGSSFYYSFMFLPAERRQAITALYAFCREVD
                     DVVDECHDLSLAQTKLEWWRQEVGRIYGGLPTHPVGHALKDVLKGFRLPQEQLLEIID
                     GMAMDLSQTRYLDFKGLQLYCYRVASVVGLLAAEIFGYQDRQTLKYAHDLGLAFQLTN
                     IIRDVGEDARRGRIYLPIEDLQRFNVPAKDLLEARYSDAFRELMAFQAERAEKFYDQA
                     FAQLPAIDRKAQRPGLVMAAIYRTLLREIARDGFLVLDRRTSLTPLRKVWLAGTTWFR
                     A"
     gene            complement(11145..11993)
                     /gene="hpnC"
                     /locus_tag="PKN25_06710"
     CDS             complement(11145..11993)
                     /gene="hpnC"
                     /locus_tag="PKN25_06710"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HNV90624.1"
                     /translation="MPVEHYENFPVASLLLPARLREPVEAIYAFARGADDVADEGDAP
                     AVARLARLHDYQLGLEACAQGRPVADATLAPMFARLGRAIHTHALPLQPFRDLLDAFM
                     QDVGKTRYADFAELRDYCRRSADPVGRLMLHLYGAADAENLRRSDAICTALQLINFWQ
                     DVAVDWEKRRIYLPLDDLARFGLDEPALDALARGERSPGEDWRALMAFEVRRARTMML
                     DGAPLARTLRGRVGWELRLVVLGGLRILEGIEAVGYDVFHHRPALGRGDWARLGWRAL
                     NYGKLG"
     gene            12261..12938
                     /locus_tag="PKN25_06715"
     CDS             12261..12938
                     /locus_tag="PKN25_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90625.1"
                     /translation="MLQLDDVTRILPGAPARVLFERLALRVKAGECVAIVGESGSGKS
                     TLLNCIAGLEPIDGGTIRVGETPIERLDDDAMARLRRSHFGFVFQAFHILPHLNLLQN
                     VALPLWLLGIAEAEAARRAQEMLGRVGLGARAPDPPQQLSGGELQRVAIARALVHRPH
                     LVLADEPTGNLDPGRASEVLDLLLGQIRDSRAAGVMVTHSRSAAARADRVLELRADGL
                     HDVTGHP"
     gene            12935..15487
                     /locus_tag="PKN25_06720"
     CDS             12935..15487
                     /locus_tag="PKN25_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNV90626.1"
                     /translation="MSVPLRRIFLASLLRRRLASALSLLAIALGVALGLAVHLIHGAA
                     LDEFGRGMRAVAGKADLQVVGPRDGFDEALFATLARRPEVAAASPVLEIEARLPGREE
                     SLRILGVDLFRVLRVQPGLMPLAEGGGDLRFAALRPGVVFLSDDARQRLSAPGRSMEG
                     EVLPPALVDARIAVQSGVRSLVLEAGGRVPGAGGVLGVMDLGAAQQAFARIGVLSRID
                     LRLAEGVSPAAAQAALSALLPPGIALRTPEAAAGELGSLSRAYRVNLTMLAAIALLTG
                     GFLVFSTQLLSVARRRREFALLRALGLVRAELMRGLLAEGATVGVVGGLLGVVLGHAL
                     AAGAFRLVGGDLGAGFFRGVAPQLAFDPAAGLAYLLLGVAAGVAGTWLPARAAAAVAP
                     ARALRAGEGERAGLPRGDGRGRLGAAMLVLAALACLLPPFGGIPLGGYAAVVFVLAAA
                     ILALPVLARLVLPLLARGRSAIWRLAHARLAAAPGQAVVAGAGVIASVALAVSMAIMV
                     NSFRVSVDEWLAQVLPADLYVRASAASGSGYLDPPALVAIAATPGVARVETTRLVNLR
                     VSDTEPLFSVLARATQGNRGLPLVSGSMEPVAGDAEGLPPAWVSEAAADRHDLARGSR
                     FELPLGGRMQAFRVAGVWRDYARQHGAALIERRDYVALTGDDRVNDAALLLEPGATPS
                     AVAQALRERFGAEHITIALPGEIRALSLQIFDRTFLITYLMEAVAVVIGLFGIATTFA
                     ALAATRQGEFGMLRHLGFTRAEIGRLIATEGALTAGLGVIAGLAGGAAIAWVLIEIIN
                     RQSFHWSMELAVPWSGLAIFAVCLVLLAALVARLAGRHAMRRSAVLAVKADW"
     gene            15612..16685
                     /locus_tag="PKN25_06725"
     CDS             15612..16685
                     /locus_tag="PKN25_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HNV90627.1"
                     /translation="MLVCGGAAQAQDSGPPYPPVVPGKALVFPQDFGAHPAYRTEWWY
                     ITGWLRDAAGVERGFQLTFFRVRTRIGEDNPSRFAPRQLVLAHAAVADPATGRLRHAE
                     RSGRAYPGLVEALEGRTAVRVDDWFLEGGESPAAGYRSRIEAEDFGFELEFTPDRAPV
                     PNGRDGYSQKAPDPLNASYYYSRPQLAVSGSLRLDGEPVAVSGHAWLDHEWSSEILPE
                     GARGWDWIGINLGDGGGLMAFRMRDHEGRALWAAATTSTRAGSVRIHPPTEVRFEPLR
                     RWRSPRTGIEYPVEWAVTVGERRYRLRPLMDDQELDSRASTGAIYWEGAVRLHEAGGG
                     AGEGPELGRGYLEMTGYAERLRM"
     gene            complement(16851..17435)
                     /locus_tag="PKN25_06730"
     CDS             complement(16851..17435)
                     /locus_tag="PKN25_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90628.1"
                     /translation="MEINQKKRSNRLKFTFNDDSFNYAYKDRSGSGDIDVQYAGFPTK
                     ASVSIERNEWLRNAGLIWCVIGVLQLGHAMVTGDSLQGRGFWLLIGVICLVWYRWATV
                     KYSVFRSQAGNVLVIQDREHDRIVDEINRRRKDQLLALYGELDMRNTLEDEIAKFSWL
                     HEQDVLSKDELEGRLAQARAAFQAPGPAASSQLH"
     gene            complement(17469..18581)
                     /locus_tag="PKN25_06735"
     CDS             complement(17469..18581)
                     /locus_tag="PKN25_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90629.1"
                     /translation="MQHSYSFDLVLGHLRLLQQGSDLTSDARWAADESPEACATLSTR
                     ARETVERLKKAFPDLQPLPVEAEEVPDLLGRVQDPEVSIRRLHRFVRLVGTWECGSVL
                     IGTYDNSIEVSLAVDAERLNSGNLNESLARLLTSLESATHFNFLDQEGRRTLEPVSAA
                     ALLLERHHKGLLALQRATRRSRRLQALAAPSAILMTIVVATLAFLIVRGAVEHGSLVL
                     RTDSARDAVFVTTALTPPASGFSLSPGYTLEGHILETGERARLAVSRDVYIRAAPGAR
                     HTVLRTNDPATPWVLRATFNPDEAVLRVGDGGVAWFALVALLPVALWGVLVFYPWVRV
                     RGEGRALMKEQMSRNLLSITKLTLLLGVVALIKRLA"
     gene            18573..19301
                     /locus_tag="PKN25_06740"
     CDS             18573..19301
                     /locus_tag="PKN25_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90630.1"
                     /translation="MLHAFPADQELPWIMPKNSKPTRYNRLVMGMLLSFLATGHSQAN
                     DHAAGRTLRPTAVALHIHEEIADQRFVPKLVARLQGSLAPPIVTIPTKMDLAPFRPTL
                     GLMDARPLLDALIGSIDWRGDGTVVRVVVIADDMQLKPARFNFAASAGDTSTPFHLSI
                     VSLARLQQLTDRGRVDRDPDRTAQRAFKLIAKNTARLMGYASSDACLFAFPRSLSELD
                     ATPENFCEPDLSALVAAGIARSPQ"
     gene            19426..20496
                     /gene="rfbB"
                     /locus_tag="PKN25_06745"
     CDS             19426..20496
                     /gene="rfbB"
                     /locus_tag="PKN25_06745"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNV90631.1"
                     /translation="MILVTGGAGFIGANFVLDWLQGSEEPVINLDALTYAGNRETLAR
                     LEGDARHLFVHGDICDRALVERLFAEYKPRAVVHFAAESHVDRSIHGPGAFVRTNVDG
                     TFTLLEAARAHWSALPAGEREAFRFLHVSTDEVYGSLGPNDPAFTETKAYEPNSPYSA
                     SKAASDHLVRAWHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIVNALAGKPLPIYGDG
                     QNVRDWLYVGDHCSAIREVLARGRLGETYNVGGWNEMANLEIVHTLCALLDELRPSAA
                     GSHARLITYVKDRPGHDRRYAIDARKIERELGWRPAETFQSGIRKTVRWYLDNPAWIA
                     NVQSGAYRDWVARNYAGREARA"
     gene            20493..21383
                     /gene="rfbD"
                     /locus_tag="PKN25_06750"
     CDS             20493..21383
                     /gene="rfbD"
                     /locus_tag="PKN25_06750"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNV90632.1"
                     /translation="MKILLLGKNGQVGWELQRALAPVGTVVALDRGGADGLHGDLEDL
                     DGIDRTVRSLAPDVIVNAAAYTAVDKAETDVERAQRINAEAPGVLARAAATVGALLVH
                     YSTDYVFDGSGDTPWRENAPTAPLSVYGRSKLAGEEAIRAAGCRHLILRTSWVYAARG
                     GNFARTMLRLAAERERLTVIADQHGAPTGAELIADVSAHALRAEHADRSLGGTYHLAA
                     GGETSWHGYASFVIEQARKLGATLKAGEIAPIGTRDYPTAAARPLNSRLDTSRLRERF
                     GLALPDWRDGVARMLREILD"
     gene            21425..22873
                     /locus_tag="PKN25_06755"
     CDS             21425..22873
                     /locus_tag="PKN25_06755"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNV90633.1"
                     /translation="MTIKSVILSGGSGTRLWPASRESYPKQLLPLTGERSLLQETALR
                     LKDFPGGEVDPRPLVVTNEEYRFIIAEQLRQIGVRSPQIVLEPVGRNTAPALTLAALV
                     AVEEGDPILLVMPADHVITEQVAFQHAIAIGAKAAAKGALVTFGIVPDRPETGYGYLR
                     SGEALAEGEGARALLEFVEKPDRATAEAYVASGAYYWNSGIFMMKASVWLAAIRRFAP
                     EMATACTTAVTGCTHDADFIRLDTAAFERSPSDSIDYAVMEKLAEAPELGQGVVVPLS
                     AGWSDVGAWDALWAVSAKDGEGNAARGEVLFEACRNTLVHAGSRLVAAVGCEDMVVVE
                     TPDAVMVAHKDRTQDVKKVVARLKAEGRTLTQSHRKVYRPWGWYDSIDAGERFQVKRI
                     VVNPGAKLSLQMHHHRAEHWIVVKGTAEVTNGERSFLLAENESTYIPLGHTHRLSNPG
                     KVPLEIIEVQSGSYLGEDDIVRFEDTYGRSTA"
     gene            22952..23036
                     /locus_tag="PKN25_06760"
     tRNA            22952..23036
                     /locus_tag="PKN25_06760"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22986..22988,aa:Leu,seq:tag)
BASE COUNT         3633 a         8112 c         8027 g         3488 t
ORIGIN      
        1 cgttaacttc gggctcaccg ccgaccgcac cgagatcacc acctacaacg cgagtccgct
       61 gctctacaag gatttctgca aggaaaacgg cctcgacaac cccagcggcg tcggcgactg
      121 cacggttgac agcctgctgt tgaccgtcgg ctggtcgcgc gacggtcgcg acagcttcac
      181 ctatccccgc agcggcgtgt atcagcgtgt ctatggcgag gtcaccgtcc cggttggcga
      241 tctgcgttac ggcaaactcg gctaccagta ccagcactgg ttcccgatcg gaaccgacta
      301 cgccctgatg ctcaacgcgg acgtcggcta cgccaagggc ttcggcggca aggacgtacc
      361 cttctacaag aactactacg tcggcggcat cggctcggta cgcggcttcg accaaggctc
      421 ggtcggcccc aagtacaagg ataccgacgg cgacatcagc tcgaccggcg gcacccgcaa
      481 ggccgtcgcc aacgccgagt tcttcttccc gatgccgggc gcgggcaagg accgttcctt
      541 ccgcatctcg gccttcctcg atgccggcta cgtctggggc agggacgaag aaccgctcgg
      601 cggcggcact ttcgtggatc agaacatccg tttcagcgat ctgcgctaca gtaccggctt
      661 ggcattctcg tggagctcgc cgatcggccc gttgaagttc agcttcggcc atccgctcaa
      721 gaaggaagag aacgacgaga tccagcggtt ccagttccag ctcggcagcg tgttctgagc
      781 tgcgggcgaa acagtttcat ccggaggcat aattgaaagt cagtaccctc tccctggtcg
      841 cactcgctgt gctcggcctt tcccagaccg cgttcgccga aaccaagatc ggcttcgtca
      901 attccgatcg cgtcatgcgc gaagcagcgc ccgccgtgcg ggcgcagcag cggctggaga
      961 aggagttcga aaagcgcgac caggagctgc agcgcatcgc gcgcgacctc aagtcgatgc
     1021 aggaagacct cgagcgcaac ggcccgacca tgggcgacag cgaccgtcgc aacaaggaac
     1081 gtgcgttcaa cgagctcaac cgcgacttcc agcgcaagca gcgcgagttc cgcgaagacc
     1141 tgaaccaacg ccgtaacgag gaactcgcct cggtgctcga caaggccaac cgctcggtca
     1201 agcagatcgc cgagcaggaa aactacgaca tcatcttcca ggaagcggtg tacgcgaacc
     1261 cgcgcatcga catcaccgac aaggtcatca agaccctgtc cgacgccaag taagactggt
     1321 cgcccgatgt tccgactgca cgagctggtc gatcgcctgg gcggcgagat cgcgggagac
     1381 ggcgatgtcg cggtttcgcg cgtcggcacg ctggacaagg cgggggaggg cgatctcgcc
     1441 ttccttgcca accgcaagta cctgtcgcag gtcggcgagt gtgcggcaag tgcgctgatc
     1501 gtcacgcccg acctgcgcga ggcgctggcc gggcgcaacc tgatcctgac cgccgacccc
     1561 tacctgtact tcgcacgcgt cgcgcagctg ttcaatccgc cccccgcggt gcgtcccggt
     1621 atccatgcgc tcgccgcggt cgccagcacc ctgccggcca gcgtgcaggt gggacccggc
     1681 gcctcgatcg aggaagacgt ggtgctcggc gaggacgtgg tgatcgggcc caactgccac
     1741 gtggggcgcg gcactcgcat cgggcgcggc acccggctct atgccaacgt cagcatctac
     1801 cacgactgcg tgatcggcga ggactgcatc ctgcattcgg gcgtggtcat cggcgcagac
     1861 ggcttcggct tcgcgcgcga gaagtccggc gcctgggtca agatcccgca gaccggccgt
     1921 gtggtcctcg gcaacgacgt cgagatcggc gccaacacga cggtggaccg cggtgcgctc
     1981 gacgacaccg tgatcggcga tggcgtcaag ctcgacaacc tcatccagat cgcgcacaac
     2041 gtgcgcgtgg gcgagcacac gatcatggcc ggctgcgccg gggtcgcggg cagcgcccgg
     2101 atcggcgcgc gctgcatgat cggcggccag gccggcatct ccggacacct gagcatcgcc
     2161 gatgacgtcg tggtgtcggc gtggaccctg gtcgccaagt cgatcgccaa accgggcgtc
     2221 tacaccggca acctgccgct gcagacgcat ggcgactggg tgaagaactt ctcccacctg
     2281 cgccacctcg acgcgctcgc caggcgcgtc cgccaactcg aacaaaacag cgacggtccc
     2341 gacgaccgct cgcagggctg agccatcatg gacatcaagg aaatcctgca gtacctgccg
     2401 caccgctatc ccttcctgct cgtcgatcgc atgctggaga tcgaaccggg cgtgcgtgcg
     2461 cgcgggctga agaacgtgac catgaacgag cccttctttc cggggcactt cccgcaccac
     2521 ccggtgatgc cgggcgtgct gatcatcgag gccatggccc aggctgcggc gctgctgtcg
     2581 ttcaagagca ccggcgtgaa gcccgacgac aactcggtgg tgtacttcgc gggcatcgac
     2641 aacgcgcgct tcaagcgtcc ggtggtgccg ggcgaccaac tgatcttcga cgtcgagatc
     2701 acccacgcca agcgcaacat ctacaagtac aaggcacgtg ccagcgtcga cggcgagctc
     2761 gccgccgaag ccgagctgat gtgcgcgatc aagacgctat gattcacccg accgccatcg
     2821 tccatccggg tgcgaagctc ggcgccaacg tcagcgtcgg ggcctattcg ctgatcggcg
     2881 agaacgtcga gatcggcgac ggcacgcgca tcggcccgca cgtcgtggtc gagggccaca
     2941 cccgcatcgg ccgcgacaac gagatcttcc agttctgttc gatcggcgcc agcccgcaag
     3001 acaagaagta cgacgccgag cccacccggc tcgagatcgg cgaccgcaac acgatccgcg
     3061 aattttgctc gttcaacgtc ggcaccagcc aggacgcgca cgtcacccgc gtcggcaacg
     3121 acaactggat catggcctac gtgcacatcg cgcacgactg ccaggtcggc gaccacacca
     3181 tcttcgccaa caacgccacg ctcgccggcc atgtccatgt gggcgactgg gcgatcctcg
     3241 gcggcttcac cggggtgcat cagttctgcc gcgtcggcgc gcacagcttc tgcggtgtcg
     3301 gtaccgtgct gctgcaggat ctgccgccct tcgtgaccgt ggcgggcaac ccggccaaac
     3361 cgcacggcat caacagcgaa ggcctcaagc gccgcggcta cagtgccgag ggcatcgccg
     3421 cgatcaaacg cgcctaccgc gcgctctacc gcagcggcct cacgctcgac gaggcgcgcc
     3481 agcgcgtcgc cgagatcgcc gccgggcagg ccgaggccgc gcccttcgcc gccttcatcg
     3541 cggactccgg tcgcggcatc gtgcgctgag catgatccgc tgcacggcgc gggccgcgtc
     3601 tcgaacggca tgaagatccg catcgccatg gtcgccgggg agacctccgg cgacctgctc
     3661 gcgagccacc tgatccgagc gctccgccgc catctgcccg acgcggagtt cttcggcatc
     3721 ggcgggccga agatgcaggc cgaaggcttc gacgtgcgct ggccctgcga gctgctcgcg
     3781 gtgcatggct acgtcgacgc gctcaagcgc taccgcgagc tctccggcat ccgccgcgcg
     3841 ctgctcgcgc agatccgcgc cgagcgcccc gatgccttca tcggcgtgga tgcgcccgat
     3901 ttcaacctgt ggctcgaagg caaggtgcgc gacgccggca tcccggcgat ccatttcgtc
     3961 ggcccgtcga tctgggcctg gcggggcggg cggatcaagc gcatcgcgcg ctcggtgtcg
     4021 cacatgctgt gcctcttccc cttcgagccc gagctctacg agcgtgccgg cgtgccggtg
     4081 agctatgtcg gccacccgct cgccgacgaa tttccgctcg agcccgaccg cgccgctgcg
     4141 cgcgaacgcc tcggcattcc tctcgagcgc ggcgtggtgg cgatgctgcc ggggagccga
     4201 cagtccgaag tgcgcaacct ggccgacatc ttcatcggca ccgcgaagac tttgcacgaa
     4261 cgcgatccgg agcgcctctt cctggtcccg ctggccaccc gcgagacgcg ccagctcttc
     4321 gaggaagcgc tgcaccgcaa cgatgcgggc gggctgccga tccgcatgct gttcggtcac
     4381 gccgtcgagg cgatgaccgc agccgacgtg gtgctggtgg cgagcggaac ggcaagcctg
     4441 gaggcggcgc tgctcaagcg cccgatggtg atcacctacc gcatcggcaa gtggcagtac
     4501 cacctcatga aacgaatggc ctacctgccc tgggtgggcc tgcccaacat cctgtgcggc
     4561 gagaccgtgg tgccggagct tctccaggac gaggccgacc ccgaacacct tgccgccgcg
     4621 atcgacgact ggttcgccga cgatgcgcgg cgcgctgcgg tcgcggcgcg cttctccgac
     4681 ctgcatcgca ccctccggca ggacaccgcg cggcgcgccg cggaggcgat cctgccctat
     4741 ctggacaagg cgggaaaagc gtcgtgaccg gcgacctgtt cgcgcccgac ctgtcagcct
     4801 ccggcctggt ctgcggggtc gacgaggctg gccgcgggcc gctgtgcggg ccagtggtcg
     4861 ccgctgcggt catcctcgat cccgcgcgcc cgatcgaagg cctcgccgac tccaagaagc
     4921 tgagcgaggc cgcgcgcgag cgcctcgcac cgctgatccg cgcgcgcgcg ctggcctggg
     4981 cggtggccga ggcgagcgtc gaggagatcg accgcctcaa catcctgcac gccaccatgc
     5041 tggccatgca gcgcgcggtg atggcgctcg ggatcgcgcc ggccgaggtg cgcatcgacg
     5101 gcaaccgctg ccccaagctg ccctatccgg cgcgggcgat cgtcaagggc gacgccaccg
     5161 agccatcgat ctcggcggcc tccatcctcg ccaagaccgc acgcgatgcg gccatgcgcg
     5221 agctcgacgc gcagtggccg cagtacgggc tggccgctca caagggctac ccgaccgccg
     5281 cccaccttgc ggcgctcgcg gcgcacggcg tgcaggcctt ccaccggcgc agcttcgcgc
     5341 cggtgaagaa gctgctcgag cgccgggatg agtgagccgc agctcaaggc gctggcctcg
     5401 cgtgacaacg ccttcgtcaa gcgcctgcat gcgctcgcgc actcggcacg cgaccgccgc
     5461 aagctcgacg agaccgtgct cgacggcgcc cacctggtcg aatccgcgct cgcggcgggc
     5521 gtggccttgc gcgcgctggt ggtgtcggag tccggcctgc tgcgcgagga gcatcgtcgc
     5581 ctgctcgcgg cggcgccggc agaggtgccg cgctaccgcc tgggcgatgc cttgttcgcg
     5641 catgtcagcc cggtcgacag ccccgccggc ttgctggcca ccatcgccat gccggcgagc
     5701 acgccgagcg ccccgggcga cgccagcctg atcgtggtcg acggcgtgca ggaccccggc
     5761 aacctcggca gcctgctgcg caccgcggcc gcggccggca tccgccacgc gctgctcggt
     5821 gagggctgcg cgcaggcctg gtcgccgcgc gcgctgcgtg ccggcatggg cgcgcagttc
     5881 gttgtccgga tcgaggaggg ctgcgaactg ctcgcccgac tggagagcta cccgggtctg
     5941 tgcctggcca ctgccctgcg cgaggacgcc cacccgctct acgagctcga cctgcgcggc
     6001 ccggtggcct ggcttttcgg ggcggaaggg cagggacttt ccccggccct gctggcgcgc
     6061 gccgacggct gcgtgatcat cccgatggcg gccggcatcg aatccctcaa cgtcgccgcg
     6121 gccgcggcgg tctgtctgtt cgagcaggcg cgtcagctcc gcgccacggg gacgatccga
     6181 ggcgtgaatt ttccttcttg actcgggcgc aggcttgctt ccatgctttc cgcattccaa
     6241 gcggggcacg gcggacgccg cgacagaccc cgaggacgag accaggggtg gtagcgcccc
     6301 gcgaggagga tgatggaaaa gtacgccaag acacccaagg gacaggacga gatcgcgcat
     6361 cgcacgcacg gcctcgggct gcgcgcccgc cgcctgctga tcctggtcga tggacagcgc
     6421 agcgtcgagg atctgtgtgc ccttaccggc gacgcgaaga ttgcggctgc gctcggagaa
     6481 ctcgccgacg cagggttcat cgcgcctagc gccgggatga ccgacccgcc cgaagccagc
     6541 atacgcgaag gacagccggc ggcaggcgcg gcgccggtcg cgcgggcgca caccggccac
     6601 acggccagcc tgccgctcgc gcgcgacttc atgatcaaca ccctgcgcac cttccacggc
     6661 ccctacggca agctcggcct ggtcaagcgt gtccatgcga cccggagcag cagcgaactg
     6721 cgcgcgctga tcgccgaatg gaaggagtcg atcgacgagt cacgcctcgg ccgcaagcgg
     6781 gcggacgagc tgctcgcgct gctggaggag gtgctgccgg gctgaaggcg gtgttgcggg
     6841 gcgattcgcg ttaacatccg gccatccggc tccgcgccga cacagcgaac agaaggcatg
     6901 atcctcgtca tctcccctgc caaggcgctc gactacgaga cgccgatcac cgtctccaac
     6961 tacacccggc ccgacttcct cgacgattcc gcagcgctga tcgaggtact gcgcgagaaa
     7021 tcgcccgccg agatcgccga gctgatgtcg ctctcggatc cactctccac gctcaacgtg
     7081 gcgcgctacg cgagctggtc gcgcccattc agccccgaca acgcacggca ggcgctgctg
     7141 gccttcaacg gcgacgtcta cgagggactc gatgcgagga gcctgaccgc ggacgaactt
     7201 ggctgggcgc aggaccacct gcgcatcctc tccggcctct acggcgtgct gcgcccgctc
     7261 gacctgatgc aggcctaccg cctggagatg ggcacccggc tggccaaccc gcgcggcaag
     7321 aacctctacg agttctgggg cgagcgcatc accgacgcgc tcaacggcct gctcgcgcgc
     7381 gaggaagacg ccgggcgcga gcgtgtgctg ctgaacctcg cctccgacga gtacttcaag
     7441 tcggtcaagc ccaagaagct gaaggggcgc atcgtcaccc cggtgttcga ggactggaag
     7501 ggcgggcgct acaagatcgt cagcttctac gccaagcgcg cgcgcgggct gatgagccgt
     7561 tacgtcatca cgaatcgcat cgacgaggtc gacgcactgc agggcttcga cgccgagggc
     7621 tacgccttcg ccgccgacgc gtccgacgcc gataccctgg tcttccgccg tcgcgaggag
     7681 tgaacgtgct gaagatcagc tccaccttcg actccggcgc gatcgaggtc gtgcgcctcg
     7741 acgatccggc cgacatccgc ctgcgcctgc gcaaggacaa cgccgccgac ttccaccagt
     7801 ggttccactt tcgcctgatg ggcgcggccg gcaagccggt gcgcatggtg ttcgagaacg
     7861 ccgcgggcgc ggcctatccg gacggatggc gggactaccg ctgcgtcgcc tcctacgacc
     7921 gccagaactg gttccgcatc gccggcacgc gctacgagga cgggcagctg atcgtcgagc
     7981 acacgcccga gcgcgacagc ctctactacg cctacttcga gccctattcg cacgagcgtc
     8041 acctcgacct gctcgcgcgc gtcgagctct cgccctacgc ccgcgtgcgc aacctcggca
     8101 gtacggtgga tgggcgggac ctcgacgtgg tgacggtcgg gcgcgacgcg ccgggacggg
     8161 tgccggtgtg gatcatcgcg cgccagcatc ccggcgagac catggcggaa tggttcgtcg
     8221 agggcctcct cgagcgcctg ctcgacaatg ccgacccggt cgcgcgcaag gtgcgcgagc
     8281 acgccgtgct gcacatcgtg ccgaacatga acccggacgg cgcggtgcgc ggcaacctgc
     8341 gcaccaacgc cgccgggcgc aacctcaacc gcgaatggcg cgcgcccgac cccgtcgcca
     8401 gccccgaggt cttcctggtg cgcgcggaaa tggagcgcac cggctgcgcg ctcttcctcg
     8461 acatccacgg cgacgagtcg ctgccctacg tgttcttctc caccgccgag gaggtgcctg
     8521 gcttcaccgc cgcggcggcg gccgcgcagg cgcgcttcgt cgagaccctc ctcatggtca
     8581 gccccgactt ccagacgcag gagggctaca agcccggccg cttcggcgag gaactgctga
     8641 cgctggcgag caagtgggtc gccaaccagt tcggctgcgt gtcgctgacc ctggagatgc
     8701 cgttcaagga caacatcatc ctgcccgacg gccacgtcgg ttggaacggt gcgcgcagca
     8761 agcgcctggg cgcggccatg ctcgatccga tcctgcgcca cctgcgtgag ggcggcgaag
     8821 gctgatcggg aggggaggcg gtggcgagtg gacgtggcgg cgacgtgcag ctgcgtcccc
     8881 gcttcacggc cgcgaggccg cggccggatt caccgagcgg atcaggcctt gtgcccgatc
     8941 tcggcgagga tcgcggtcgc gcaggccaca cccgagcgca ccgcggattc cagcgtcgcg
     9001 gggtagtccg aatccaggta atcgccggcc agccacagtc cgggcagcgg ggtgcggatg
     9061 ccggggcgga agacgcctgg ccgacacgcg aaagtggcgc gcttttcgac gatgacctgc
     9121 gaccactccg gggctggcag acggcgaccg agctggcgtt cgagctgggc gtgcaccgcg
     9181 aggacgagct cgtcgcgcgc gagcgcctcg tgcgggccgt gggcgctgat cacgcacgag
     9241 aagaggccct ccggcccgcc gagctggccg cggtcgaaga cccactgcgc cggcccggcg
     9301 gtgctgcccg aggccaggcc gatcatcggc tcggggagct gctggccgcg tccgagggcg
     9361 agatagacgg acacgatcgg ctcgtgctcg agcgcgtcga tctgcgccac caggcgctcg
     9421 caaccgccga cggaggcgag cagcgctccg gcatggtagg gcgcggtggc caccaccacg
     9481 tgaggccagg ggtggctgcc ggggtcgccg tcgaggcggt agccgccggc cacccgttcg
     9541 acggcctcga tcgcgttgcc ggtgcgcagc ctgccgcggc gcacaccgat gtagcgcgcc
     9601 gccggcaccg ggaagagctc ggacaggtcg gtgcgcggca gcagcagctc gctggcggcg
     9661 gcacccgcac cgaggctgtc gcgcagcacg ttcgcgaaga cctgggccga ggccgcggcg
     9721 gccggggtgt tgagcgccgc cacgcacagc ggctcccaga tcagctggca cagggtcgga
     9781 gtctggcggg tctcggcgag cagggcgtcg accgtcatcc ccggatcggg caggcggaag
     9841 ccgcgtcgct tcaaccagcg catcaggcgc agcatcgcca ggcggtcggc ccagtgcagc
     9901 ccctgcgcac gcagcaaccc cacggcgagg tggaaaggcg ccggcaggga cgcggccttc
     9961 aggtgcaggc ggccggggac gtgcagggtc agcggcaggt gggcgagctg cttgggcgac
    10021 acgccggtga ggcgcagcag gcgggtaagc tcgctgtagg cgccgatcag gatgtgctgg
    10081 ccgttgtcca cccgccagtg gtggtccttg tgcaccacgc gggcgcgtcc gcccatggtg
    10141 tgcgagcgtt cgaacacggt gacatgcacc ccggcgcggg ccagctcgac cccgcaggcg
    10201 agcccggcgt agcccgcgcc gatgatggcg acgaccggca cgctcatggc cggaggttcc
    10261 ggctgccgtg ccagctcggt cccgcgcgct gggtgcgctt ccctgcgggc gtgctcatgc
    10321 gcggaaccag gtcgtgcccg cgagccacac cttgcgcagc ggggtcaggg aggtgcggcg
    10381 gtcgagcacc aggaaaccgt cgcgcgcgat ttcgcgcagc agggtgcggt agatcgccgc
    10441 catcaccagg ccggggcgct gcgccttgcg gtcgatcgcg ggcaactggg cgaaggcctg
    10501 gtcgtagaac ttctccgcgc gctcggcctg gaaggccatg agctcgcgga aggcgtcgga
    10561 gtagcgcgcc tcgaggagat ccttggcggg cacgttgaag cgctgcaggt cctcgatcgg
    10621 caggtagatg cgcccgcggc gggcgtcctc gccgacgtcg cggatgatgt tggtgagctg
    10681 gaaggccagc ccgaggtcgt gcgcatactt gagcgtctgg cggtcctggt agccgaagat
    10741 ctcggcagcg agcaggccga cgacgctggc gacgcggtag cagtagagct gcaggccctt
    10801 gaagtcgagg tagcgcgtct gcgacaggtc catcgccatg ccgtcgatga tctcgagcag
    10861 ctgctcctgc ggcaggcgga agcccttcag cacgtccttg agcgcatgtc cgacagggtg
    10921 ggtgggcagg ccgccgtaga tgcgcccgac ctcctggcgc caccactcga gcttggtctg
    10981 cgcgagcgag aggtcgtggc attcgtcgac cacgtcgtcc acctcgcggc agaaagcgta
    11041 cagggcggtg atcgcctggc ggcgctcggc gggcaggaac atgaagctgt agtagaagct
    11101 cgagccgctc ttggcggcct tgtcctggca gtagtcgtgt ggattcatcc gagttttccg
    11161 taattcagcg cgcgccagcc caggcgcgcc cagtcgccgc gaccaagcgc ggggcggtgg
    11221 tggaagacat cgtagccgac cgcctcgatc ccttcgagaa tgcgcagccc gcccagcacg
    11281 accagtcgca actcccagcc gacgcgcccg cgcagggtac gggcgagcgg ggcgccgtcg
    11341 agcatcatcg tgcgagcacg ccgcacctcg aaggccatca gcgcgcgcca gtcctcgccc
    11401 gggctgcgtt cgccgcgggc gagcgcgtcg agcgcgggct cgtcgaggcc aaagcgggcg
    11461 agatcgtcga gcggcaggta gatgcgccgc ttttcccagt cgaccgcgac gtcctgccag
    11521 aagttgatca gctgcagggc ggtgcagatc gcgtccgagc ggcgcaggtt ctccgcgtcc
    11581 gccgcgccat acagatgcag catcaggcgc cccaccgggt cggccgagcg ccggcagtag
    11641 tcgcgcagct ctgcgaagtc ggcgtagcgc gtcttgccga cgtcctgcat gaaggcgtcg
    11701 agcaggtcgc ggaaaggctg cagcggcagc gcatgggtat gtatcgcacg gccgaggcgg
    11761 gcgaacatcg gcgccagcgt cgcgtcggcc accggccggc cctgcgcgca ggcctcgagc
    11821 ccgagctggt agtcgtgcag gcgcgccaac cgcgccaccg cgggcgcgtc gccctcgtcg
    11881 gcgacgtcgt cggccccgcg cgcgaaggcg tagatcgcct ccaccggctc gcgcaggcgg
    11941 gccggcaaca gcagggaggc gacagggaag ttctcgtagt gctcgaccgg catcggggca
    12001 aaggagcggc gggatccgca gggtgccgca gtatatccgc acgccccgtg gagaagcggc
    12061 gcaagccgcg aacagggcgg ttcgccaatg ggggcttcgc aatctccacc gccgcattcg
    12121 cgccttgcgg cgctcctacc ggaggcatcg caccgggaag cgcatgcagt agcggcgcaa
    12181 gccgcgaacc ttttgcgcag ggcaatgaca caatgtcgtc catctttccg ctctccagcc
    12241 ccgtgccacg aggccttccg atgctgcagc tcgacgacgt cacccgcatc ctgcccggcg
    12301 cccccgcgcg cgtgctgttc gagcgccttg cgctgcgggt caaggcaggg gagtgcgtgg
    12361 cgatcgtcgg cgagtcgggc tcgggcaagt cgaccctgct caactgcatc gccggcctgg
    12421 aaccgatcga cggcggcacg atccgggtcg gcgaaacacc catcgagcgc ctcgacgacg
    12481 acgccatggc gcgcctgcgc cgtagtcact tcggtttcgt gttccaggct ttccacatcc
    12541 tgccccacct gaacctgctg cagaacgtgg cgctcccctt gtggctgctc ggcatcgcgg
    12601 aggccgaggc cgcgcgccgc gcacaagaga tgctcggccg cgtcgggctg ggcgcgcgcg
    12661 cgccggatcc gccgcagcaa ctgtccgggg gtgagctgca gcgcgtggcg atcgcgcgcg
    12721 cgctggtaca tcggcctcac ctggtgctgg cggacgagcc caccggcaac ctcgacccgg
    12781 ggcgagcgag cgaggtactc gacctgctgc tcggccagat ccgcgacagc cgcgccgccg
    12841 gcgtcatggt cacgcactcg cgcagcgcgg cggcgcgcgc cgaccgcgtg ctggaacttc
    12901 gagccgacgg gctgcatgac gtcacggggc atccgtgagc gtgcctcttc gacgcatctt
    12961 cctggccagc ctgctgcgcc gccgcctggc gagcgccctg tcgctgctgg cgatcgcgct
    13021 cggcgtggcg ctggggctgg cggtgcatct gatccacggt gccgcgctcg acgagttcgg
    13081 ccgcggcatg cgcgcggtgg cgggcaaggc cgacctgcag gtggtggggc cgcgcgacgg
    13141 gttcgacgag gccttgttcg ccaccctggc gcgccggccc gaagtggcgg cggcgagtcc
    13201 ggtgctggag atcgaggcgc gcctgcccgg gcgcgaggag agcctgcgca ttctcggcgt
    13261 ggacctcttc cgggtgctgc gcgtgcagcc cgggctgatg ccgctggcag agggcggcgg
    13321 ggacctgcgc ttcgcggcac tgcgtcccgg cgtggtgttc ctgagcgacg acgcccggca
    13381 gcgcctttcc gcgccggggc gctccatgga aggcgaggtg ctgccgccgg cgctcgtcga
    13441 cgcccgcatc gcggtgcagt cgggcgtgcg cagcctggtg ctcgaggcgg gtgggcgggt
    13501 gcctggcgca ggcggcgtgc tcggcgtcat ggacctcggc gcagcgcagc aggccttcgc
    13561 acgcatcggc gtgttgagcc ggatcgacct gcgcctggcc gagggcgttt ccccggcggc
    13621 ggcgcaggcg gccttgtccg ccctgctgcc accggggatc gccttgcgca cacccgaggc
    13681 ggcagcgggc gagctcggct cgctgtcgcg cgcgtaccgg gtgaacctga ccatgctggc
    13741 ggcgatcgcg ctgctgaccg gcggcttcct cgtgttctcc acgcagctgc tgtcggtggc
    13801 gcggcggcgg cgcgagttcg cgctgctgcg cgcgctcggt ctggtgcgcg ccgagctgat
    13861 gcgcggtctg cttgccgagg gcgcaacggt gggcgtggtc ggcgggctgc tcggggtcgt
    13921 gctcggccac gccctcgcgg cgggcgcctt ccgcctggtg gggggcgacc tcggcgcggg
    13981 cttcttccgc ggcgtggcgc cacagctcgc gttcgacccc gcggcgggcc tggcttacct
    14041 gctgctgggc gtggccgccg gtgtcgccgg cacctggctg ccggcgcgtg ccgcagccgc
    14101 ggtggcgcca gcgcgtgcct tgcgcgcggg ggagggcgag cgcgccggcc tgccgcgagg
    14161 cgacggtcgc gggcgactgg gggcggcgat gctggtgctc gctgcgctgg cctgcctgct
    14221 gccgccgttc ggcggcatcc cgctaggcgg ctatgcggcg gtggtgttcg tgctcgcggc
    14281 ggccatcctg gcgctgccgg tgctcgcgcg cctcgtgctg cccttgctcg cgcgcgggcg
    14341 cagcgcgatc tggcggctgg cgcacgcgcg cctggcggcg gcgccggggc aggcggtggt
    14401 ggcgggggca ggggtgatcg ccagcgtcgc gctcgcggtg tcgatggcga tcatggtgaa
    14461 ttccttccgc gtctcggtgg atgagtggct cgcccaggtc ttgccggcag acctgtacgt
    14521 gcgtgcctcg gcggcgagtg gcagcggcta cctcgatccg cctgcgctcg tggcgatcgc
    14581 ggccacgccc ggcgtggcgc gagtagagac gacgcggctc gtgaacctgc gcgtttcgga
    14641 caccgagccg ctgttcagcg tgctggcgcg cgcgacgcag ggcaaccggg ggctgccgct
    14701 ggtgagcgga tcgatggagc cggtggccgg ggacgcggaa ggcctgcctc cggcctgggt
    14761 gtcggaggcg gctgccgacc gccacgatct ggcgcgcggc agccgcttcg agctgccgct
    14821 gggaggccgg atgcaggcct tccgcgtcgc cggggtgtgg cgcgactacg cccgccagca
    14881 cggcgccgcg ctgatcgagc gccgcgacta cgtcgccctc accggcgacg accgggtaaa
    14941 cgacgccgcg ctactgctcg agccgggtgc caccccgtca gcggtggcgc aggcgctgcg
    15001 cgagcgcttc ggcgccgagc acatcaccat cgcgctgccg ggcgagatcc gcgcgctcag
    15061 cctgcagata ttcgatcgta ccttcctgat cacctacctg atggaggcgg tcgcggtggt
    15121 gatcggcctg ttcggcatcg ccaccacctt cgccgcgctc gccgccaccc gccagggcga
    15181 gttcggcatg ctgcgccatc tcggcttcac gcgcgccgag atcggtcgcc tgatcgccac
    15241 cgagggcgcg ctgaccgcag ggctgggcgt gatcgccgga ctcgccggcg gcgcggcgat
    15301 cgcctgggtg ctgatcgaga tcatcaaccg gcagagcttc cactggagca tggagctggc
    15361 cgtgccgtgg agcgggctgg cgatcttcgc cgtctgcctg gtgctgctgg cggcgctggt
    15421 ggcccggctc gccggaaggc acgcgatgcg gcggtcggcg gtgctggcgg tgaaggcgga
    15481 ttggtagggg gtgaggcgtg aggagtcagg agtcaggagc ggtgggggcg ggcaggctcg
    15541 cggcgggcgt tgcgcttgtg gcgctggaga ggcggcttgt ccggctgcgc gtgctgctcc
    15601 tgggagcgct gatgctggtg tgcggtgggg cagcgcaggc gcaggattcc ggaccgccct
    15661 acccgccggt cgtgccgggc aaggcgctgg tgtttccgca ggatttcggc gcacatccgg
    15721 cctatcgcac cgagtggtgg tacatcaccg gctggttgcg cgacgcggcg ggggtggagc
    15781 gcgggttcca gctcaccttc ttccgcgtgc gcacgcgcat cggcgaggac aacccgagcc
    15841 gcttcgcgcc acgccagttg gtgctcgcgc atgccgcggt ggccgacccg gcgaccggca
    15901 ggctgcgcca cgccgagcgc tcgggacgcg cctatcccgg actggtcgag gcgctggaag
    15961 ggcgtaccgc ggtacgggtg gatgactggt tcctcgaagg aggagagagc ccggcggccg
    16021 gctaccgcag ccgcatcgag gcggaggact tcggtttcga gctcgaattc accccggatc
    16081 gcgcgccggt gccgaacggg cgcgatggct acagccagaa ggcgcccgat ccgctcaacg
    16141 ccagctacta ctacagccgc ccgcagctcg cggtgagcgg ctcgctgcgc ctggatggcg
    16201 agccggtggc ggtgagcggc catgcctggc tggatcacga gtggtcgagc gagatcctgc
    16261 ccgagggcgc gcgcggctgg gactggatcg gcatcaacct cggcgacggc ggcggcctga
    16321 tggccttccg catgcgcgac cacgagggcc gcgcactctg ggcggccgcg accacgagca
    16381 cgcgcgctgg cagcgtccgc atccacccgc ccacggaggt gcgcttcgaa ccgctgcgcc
    16441 gctggcgctc gccgcgcacc ggaatcgagt acccggtgga gtgggcggtc acggtgggcg
    16501 aacggcgcta ccggctgcgc ccgctgatgg acgaccagga gctcgacagc cgcgcctcca
    16561 ccggggcgat ctattgggag ggcgcggtgc gcctgcacga ggcggggggg ggtgccgggg
    16621 agggccccga gctcgggcgc ggctacctgg agatgaccgg ctacgccgag cgcttgcgca
    16681 tgtagaaacc atgaagggcc atccgttccc tctccacctg accccgttgc acgggagctc
    16741 acggcagagc gaaattccgt ctgacgcgta gaacgccaac gcccgccgga ccagccacgc
    16801 gagcccggtg ccggcaacac ctcatcgcgc ccgattcgcc agcgcagccg tcaatgaagc
    16861 tggctggatg ctgccggccc tggcgcctga aaggccgcgc gcgcctgtgc gaggcgtcct
    16921 tccagttcgt ccttggacaa gacgtcctgc tcatgcagcc aagagaactt tgcgatctcg
    16981 tcctcgagcg tgttgcgcat gtccagttcg ccatagagag caaggagctg gtcctttcgc
    17041 cggcggttga tttcatcgac gatccgatca tgctcgcggt cctgaatcac gaggacgtta
    17101 cccgcttgcg agcggaaaac cgaatacttg acggtcgccc agcgatacca gacaaggcag
    17161 atcaccccga tgagaagcca gaagcctcgc ccctgaagcg agtcgccggt gaccatcgcg
    17221 tgccccaatt gcagcacacc gatgacgcac cagatcagtc cggcattgcg cagccattcg
    17281 ttccgctcga tcgagaccga ggccttcgtc gggaagccgg cgtactgcac gtcgatgtcg
    17341 ccggagccgc tgcggtcctt gtacgcatag ttgaagctgt cgtcgttgaa cgtgaacttc
    17401 aatcggttgg aacgcttctt ctggttgatt tccacggagt cctccggttc atgggttgtc
    17461 ttgcgccgtc aggcaagccg cttgatcagc gccacgacgc ccagcagcag tgtcagtttg
    17521 gtaatcgaca gcaggttcct gctcatctgc tccttcatca gcgctcgacc ttcaccccgg
    17581 acccgcaccc acggatagaa cacgagcacg ccccacaggg cgacgggcag cagcgcgacg
    17641 agcgcgaacc aggcgacgcc gccatctccg acccgtaaga cagcctcgtc ggggttgaac
    17701 gtcgccctca acacccaggg cgtggcggga tcgttggtgc gcaggacagt gtggcgggca
    17761 ccgggcgccg cacggatata cacatcccgc gacaccgcaa gacgagcgcg ctcgcccgtc
    17821 tccaggatgt ggccctcgag cgtgtagccg ggtgacaggc tgaagcccga cgcgggcggt
    17881 gtcagggcgg tcgtgacgaa gaccgcgtct ctggccgaat cggtgcgcag cacgaggctg
    17941 ccgtgctcga cggcgcctcg aacgatcagg aacgcgaggg tcgcgaccac tatcgtcatc
    18001 aggatggcgc taggtgctgc gagggcttgc agccgccgcg atcggcgcgt tgcgcgttgc
    18061 agcgccaaga ggcccttgtg gtgccgctcg agcagcaagg cagcggcgga caccggctcg
    18121 agcgttcgac gcccctcctg gtccaggaag ttgaagtgcg tggcggattc gaggctggtc
    18181 agcaagcgcg caagggattc attcaggttg ccgctgttca agcgctcggc gtcgacggcg
    18241 aggctcacct cgatcgagtt gtcgtaggtg ccgatcagga cgctcccgca ctcccaggtg
    18301 ccgaccaggc gtacgaagcg atgcagccgc cggatggaaa cctccggatc ctgaacgcgg
    18361 ccgagcagat ccggtacttc ttctgcctcc accggcaatg gctgcagatc gggaaacgcc
    18421 ttcttcaatc gttcgacggt ttcacgggca cgcgtggaca gggtcgcgca tgcttcagga
    18481 ctctcgtcgg ccgcccaacg cgcatcgctc gtcaggtccg agccctgctg cagaaggcgg
    18541 aggtggccga gcacgaggtc gaaactgtag ctatgctgca tgcgtttccc gccgatcagg
    18601 agctgccctg gattatgccg aagaactcaa agcccacaag gtacaaccga cttgtgatgg
    18661 gcatgttgct gtcgtttctg gctaccggcc acagccaggc caacgaccat gctgccggcc
    18721 ggaccttgcg tccgacggcg gtagcccttc acatccacga ggagatcgcc gaccagcgct
    18781 ttgttccgaa actggtcgcg cgcctgcaag gcagccttgc accaccgatc gtgacgatcc
    18841 cgaccaagat ggatctcgcg ccttttcggc caacgctcgg gctcatggat gcgcgtccgc
    18901 tcctcgatgc attgatcggc agcatcgact ggcgagggga tggaacggtc gttcgcgtcg
    18961 tcgtgatcgc cgacgacatg caattgaaac cggcgcgttt caactttgcc gccagtgcgg
    19021 gagacacgag tacgccgttt cacctttcga tcgtttcgct tgcaaggctg cagcaactca
    19081 ccgaccgtgg gcgcgtggat cgcgaccccg accgcaccgc gcaacgcgca ttcaagctga
    19141 tcgccaagaa caccgcccga ctcatgggat acgcgtcgag cgatgcctgc cttttcgcct
    19201 ttccccgcag cctttccgaa ctcgatgcca cgcctgagaa cttctgcgag ccggatctct
    19261 cggcccttgt cgcggcgggc atcgctcgat cgccgcagtg attgcccaag gacgcagcgg
    19321 tgcagcgaga ctcaaaacct gtgatccgga gtggcgtcct tcctcgtcgg ccattgccct
    19381 gaatgcgata atggcagctt tccagactcg aacaggattc cccacgtgat tctcgtgaca
    19441 ggcggcgccg gcttcatcgg cgcgaacttc gtgctcgact ggctccaggg ttcggaagag
    19501 ccggtgatca acctcgacgc gctgacctat gccggcaacc gcgagacgct cgcgcgcctg
    19561 gaaggcgacg cgcggcacct cttcgtgcat ggcgacatct gcgaccgcgc cctggtcgag
    19621 cgcctgttcg ccgaatacaa gccgcgcgcg gtggtgcact tcgccgcgga gagccacgtc
    19681 gaccgctcca tccacggtcc cggcgccttc gtgcgcacca acgtagacgg caccttcacc
    19741 ctgctcgaag cggcgcgcgc gcactggagc gcgcttcccg ccggcgagcg cgaggccttc
    19801 cgcttcctgc acgtgagcac cgacgaggtc tacggctcgc tcggcccgaa cgatcccgcc
    19861 ttcaccgaga ccaaggccta cgagcccaac agcccctact cggcgagcaa ggcggcgagc
    19921 gaccacctgg tgcgcgcctg gcatcacacc tacggcctgc cggtgctcac caccaactgc
    19981 tcgaacaact acggccccta ccagttcccc gagaagctga tcccgctgat gatcgtgaac
    20041 gcgctcgccg gcaagccgct gccgatctac ggcgacggcc agaacgtgcg cgactggctc
    20101 tacgtgggcg atcactgcag cgcgatccgc gaagtgctgg cgcgcggccg cctcggcgag
    20161 acctacaacg tcggcggatg gaacgagatg gccaacctcg agatcgtgca caccctgtgc
    20221 gcgctgctcg acgagctgcg cccgagtgca gccggcagcc acgcccgcct gatcacctat
    20281 gtcaaggatc gccccggcca cgaccgccgc tacgcgatcg acgcgcgcaa gatcgaacgc
    20341 gagctcggct ggcggccggc ggagaccttc cagagcggca tccgcaagac cgtgcggtgg
    20401 tacctggaca acccggcctg gatcgccaac gtgcagagcg gcgcctatcg cgactgggtg
    20461 gcgcgcaact acgccggacg cgaggcccgc gcatgaagat cctgctgctc ggcaagaacg
    20521 gccaggtcgg ctgggagctg cagcgcgcgc tcgcgccggt cggcaccgtc gtcgcgctcg
    20581 accgtggcgg cgccgacggc ctgcatggcg acctcgagga cctcgatggc atcgaccgca
    20641 ccgtgcgcag cctggccccc gacgtgatcg tcaacgccgc cgcctacacc gctgtcgaca
    20701 aggccgagac cgatgtcgag cgcgcgcagc gcatcaacgc ggaggcgccg ggcgtgctgg
    20761 cccgggccgc ggcgaccgtg ggcgcgctgc tggtgcatta ctcgaccgac tacgtcttcg
    20821 acggcagcgg tgacaccccc tggcgcgaga atgccccgac cgcaccgctt tccgtgtacg
    20881 gccgcagcaa gctcgccgga gaggaggcga tccgcgccgc gggctgccgt cacctgatcc
    20941 tgcgcacctc ctgggtgtat gcggcgcgcg gcggcaactt cgcccgcacg atgctgcgcc
    21001 tcgcggccga gcgcgaacgc ctgaccgtca tcgccgacca gcacggcgca cccaccggcg
    21061 cggagctgat cgccgacgtc agcgcgcatg ccctccgtgc cgaacacgcg gaccgcagcc
    21121 tgggcggcac ctatcacctc gccgcgggcg gcgagacgag ctggcatggc tacgcaagct
    21181 tcgtgattga gcaagcgcgc aagctcgggg cgacgctcaa ggcaggcgag atcgcgccga
    21241 tcggcacccg cgactatccg accgccgcgg cgcggccgct caattcacgg ctcgacacct
    21301 cgcggctgcg cgaacgcttc ggtctcgcgc tgcccgactg gcgcgacggc gtcgcccgca
    21361 tgctgcgcga gatcctggac tgaagcaccc ggccgaacga cagaacaacg caaggacccg
    21421 attcatgacc atcaagagcg tgatcctctc cggcggctcc ggcacccgac tgtggccggc
    21481 gtcgcgcgag agctatccca agcaactcct cccccttacc ggcgagcgct cgctgctgca
    21541 ggagaccgcg ctgcgcctga aggacttccc cggcggcgag gtcgacccgc gtccgctggt
    21601 cgtcaccaac gaggagtatc gcttcatcat cgccgaacaa ctccgccaga tcggcgtacg
    21661 ttcgccacag atcgtactcg agccggtggg acgcaacacc gcgccggcgc tcactttggc
    21721 cgcgctggtt gcggtggagg agggcgatcc gatcctgctg gtgatgcctg ccgaccacgt
    21781 catcaccgaa caggtcgctt tccagcatgc gatcgcgatc ggcgccaagg cggcagccaa
    21841 gggtgcgctg gtgaccttcg gcatcgtgcc cgaccgcccc gagaccggct acggctacct
    21901 ccgcagcggc gaggcgctgg cggagggcga gggcgcgcgc gcactgctcg agttcgtcga
    21961 gaaacccgac cgcgccaccg ccgaggccta cgtggcgagc ggcgcctact actggaacag
    22021 cggcatcttc atgatgaagg cctcggtgtg gttggcggcg atccgtcgct tcgcgcccga
    22081 gatggccacg gcctgcacga cggcggtcac cggctgcacc cacgacgcgg acttcatccg
    22141 tctcgacacc gccgccttcg agcgcagccc gagcgactcg atcgactacg cagtcatgga
    22201 gaagctcgcc gaagcgccgg agctcgggca gggcgtggtc gtgccgctgt cggccgggtg
    22261 gtccgacgtg ggcgcctggg acgcgctgtg ggcggtgtcg gccaaggacg gggagggtaa
    22321 cgccgcgcgc ggcgaggtcc tcttcgaggc ctgccgcaac accctggtgc acgccggcag
    22381 ccgcctggtc gccgcggtgg gctgcgagga catggtggtg gtcgagaccc ccgacgcggt
    22441 gatggtcgcg cacaaggatc gcacgcagga cgtcaagaag gtggtcgcgc gcctgaaggc
    22501 cgagggccgc acgctcaccc agagccatcg caaggtctat cgtccctggg gctggtacga
    22561 ctcgatcgac gccggcgagc gcttccaggt caagcgcatc gtggtcaatc cgggcgccaa
    22621 gctcagcctg cagatgcacc accaccgcgc cgagcactgg atcgtggtga agggtacggc
    22681 agaggtcacc aacggcgagc gcagcttcct gctcgccgag aacgaatcga cctacatccc
    22741 gctcggccac acccaccgcc tgtcgaaccc gggcaaggtc ccgctcgaga tcatcgaggt
    22801 gcagtcgggc agctacctcg gcgaggacga catcgtccgc ttcgaggata cctacggccg
    22861 cagcacggct tgagcgtcgg accagccact gccgcccggt acgcgaagcg gcgcgtgtat
    22921 aatgcgcgcc gcttcacttg tgctgaagtg tgcggggatg gcggaattgg tagacgcact
    22981 ggatttaggt tccagcgccg caaggcgtga gagttcgagt ctctttcccc gcaccagggt
    23041 tttcaatctg gcattgcacg cgcgtgctgc agcgttcccg caagcgcgct cgcggccagg
    23101 aagtccccct gcagtaggtg caactaaaac agcgagaata tgtgttatgt aaacaaatac
    23161 ccgacatggc atctcggtgc gaagaattcg ccttccagcc ttagaatccc gcctgtcctc
    23221 ctcgctcctt cctgcctccc acccctcgcg tccctcgcga
//
LOCUS       DAOQBS010000042        22842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1485_length_22842_cov_17.292096, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000042 DAOQBS010000000
VERSION     DAOQBS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 22842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22842
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1485_length_22842_cov_17.292096"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..926)
                     /locus_tag="PKN25_06765"
     CDS             complement(21..926)
                     /locus_tag="PKN25_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90634.1"
                     /translation="MSTPAIRIQGVSKHYGSLKALGGVDLEVGQGEFFGLLGPNGAGK
                     TTLISALSGLVRPDSGTLAIMGHDVVADYRNARRNLGVVPQELVFDPFFSVRELLRIQ
                     SGYFGIRNNDDWIDEILHNLDLTGKAGANMRMLSGGMKRRALVAQALVHRPPVIVLDE
                     PTAGVDVELRHGLWQFIRKLNQDGHTIVLTTHYLEEAESLCGRIAMLKAGRVVALDTT
                     ANLLQRFASHNLQVRLAAAVVLPEALAAQVVKRDGVDLELAFERFDDVGQLLAGLKSA
                     GAEIVDMAIGKPDLEQVFVGVMNQN"
     gene            complement(923..1207)
                     /locus_tag="PKN25_06770"
     CDS             complement(923..1207)
                     /locus_tag="PKN25_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV90635.1"
                     /translation="MSDPALFAPAGALTMQSAPALLAEGQRRARAGDLVVDLAAVSAA
                     DSAALALILDWMRCARAAGHALMLRNPPAGLTSLAALYDIDGLLPLERAA"
     gene            complement(1204..1866)
                     /locus_tag="PKN25_06775"
     CDS             complement(1204..1866)
                     /locus_tag="PKN25_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV90636.1"
                     /translation="MNKILDFLRAFLLCALLGAALPATASADKAPDVLVRDVTTEVLE
                     IVRADKAIQAGDTQRVIALVDAKVLPHFDFRRMTMLAVGRDWRQASAEQQTRLTDAFR
                     TLLVRTYSNALTQYRDQRIEFRPSRFAPADTRVQVRTEVVQAGAQPIGIDYMLEKGEQ
                     GWKVFDVIVAGVSLVTNYRSSFGQEIARGGIDGLIRSLEERNRALAEQQGMSQGDAGR
                     GA"
     gene            complement(1876..2727)
                     /locus_tag="PKN25_06780"
     CDS             complement(1876..2727)
                     /locus_tag="PKN25_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNV90637.1"
                     /translation="MRNVFRSSRAGLVAAALSAALATGCATTANPDDPLEGYNRAMFG
                     FNEQVDKVVIKPAAQVYEAVLPHPVRTGVGNVLGNLGDPWVALNNLLQGKVAEGMGDL
                     MRFALNSTWGLLGLLDVAGEAGLPKHDEDLGQTLGRWGVGEGAYIVLPFFGPRTVRDA
                     VALPADFIANEPFSIDHVATRNTVLGTRVVHLRSTLLGADRTLEEAALDRYVFVRDFY
                     LEQRRYKVSDGRQERVYEDFDEQSAAPPGGDVDTAAASAVGNLEHADIGASPATAGRR
                     VARQSVQ"
     gene            complement(2757..3224)
                     /gene="mlaD"
                     /locus_tag="PKN25_06785"
     CDS             complement(2757..3224)
                     /gene="mlaD"
                     /locus_tag="PKN25_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNV90638.1"
                     /translation="MSRTALDLWVGLFVAIGFAALVFLAMKVGNFSGLDGAETYRVEA
                     PFDNIGGLKVRAPVKSSGVLVGRVAVIRFDPAIYRAVVELEIDKRYAFPADTTASILT
                     SGLLGEQYVGLEAGADTAMLKGGDRVLITQSAVVLEKLIGQFMFDRAADAPAQ"
     gene            complement(3227..4015)
                     /gene="mlaE"
                     /locus_tag="PKN25_06790"
     CDS             complement(3227..4015)
                     /gene="mlaE"
                     /locus_tag="PKN25_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HNV90639.1"
                     /translation="MSAIASALRRIGALTIDGIWRLGFATRFLFAVLLNSGQSLRRIH
                     LTIRELYFNGVLSLLIIMVSGLFVGLVLGLQGYDILQRYGSADAVGVMVALSLLRELG
                     PVIAALLFASRAGSAITAEIGLMKTTEQLKAMDMMAVNPIARVVAPRFWGGVLSMPLL
                     AALFSAMGVFGGWVITVVVIGVDDGAFWSQMQAAVEFRYDVVNGVIKSAVFGTVVSLI
                     AVFEGYDCQPTAEGVSRAITRTVVTSALAILALDFVLTSFMFRG"
     gene            complement(4012..4833)
                     /locus_tag="PKN25_06795"
     CDS             complement(4012..4833)
                     /locus_tag="PKN25_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90640.1"
                     /translation="MTVSAPHPSPLVSLRDVRFAYGEREVLRGIDLSVHRGQVVAIMG
                     GSGCGKTTLLRLIGGQLRATGGTVEVEGRDVARLSGSELYALRRRMGMLFQFGALFTD
                     MSVFDNVAFPLREHTDLPAGMVRDLVLMKLQAVGLRGAAKLHPNELSGGMARRVALAR
                     AVALDPMLILYDEPFAGLDPISLGIIGQLIRKLNDALGASSVMVTHDVHESLAIVDYI
                     YFVSDGRIVAQGTPDEIRASRDPFVHQFVHAEADGPVPFHYPAEPIERLLGGEGA"
     gene            5031..6908
                     /locus_tag="PKN25_06800"
     CDS             5031..6908
                     /locus_tag="PKN25_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNV90641.1"
                     /translation="MTCTSTDLRRLLATAALLATAACAPLGRPPALEGHLDKRTAIER
                     TAPVGTATRSLAPPIAESPSSSRGAAASAATGAPPPAPARGRAAPAVRGYSIDVFDIP
                     AEQLLLAIGRDAGLELDLHAGITGNVSLQAHDRPLGELLERIARQVDLRYELRGRHLR
                     VRPDTPYIRSYAVDYVNLSRDMRGTVATSTQIATSGSAGGAGQRASSTGGAGNASITQ
                     IETRSANDFWNTLEHNLAAILAGPGRAPDCGGERRGESPAGCRNADLMLNRESGIVMA
                     RATQRQHEQLEAFLAGVQRAARRQVMIEATIVEVTLADGYRQGIDWTRLGVPSWSVRP
                     RGSGDATPGQPTLSYLSANSDIRLELLETFGTVKVLSSPRLSVLNNQTAMLKVVEEVV
                     YFLVDSTTTEYDDRKQARISATTTPQSVSVGMVMSVTPQISADGDITLNVRPTISGIS
                     GFKDDPNPSLGDIPNRVPQIRTREIESMLRLRSGEVAVLGGLMEDRIDHDTGRIPLFG
                     DLPVVGELFTRRDNNVRRTELLIFLRPLLIDEPGMRGGYAEYAGHLPDDDFLLDSPSP
                     GQRNFPHLPLRPLLAPAGAVSAAEGFSEAAYPDPADPRSPAASSTSASAANADAGTSP
                     P"
     gene            6905..8620
                     /locus_tag="PKN25_06805"
     CDS             6905..8620
                     /locus_tag="PKN25_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNV90642.1"
                     /translation="MNAPLPIGQILIAAGLIGEDQLRIALHEQRGRARPLGRVLVELG
                     FVSEAALREALAARSGLPCVDLASALADPDAIARVPQALARRHRLLPLQYDAARHRLI
                     VAMADAHDIVALDRLRAELGPDVHVELRLAGDNELGRAIEQHYGQASSIEDMVRELER
                     RAGQPIAGARDPQLVVRLVDALLAEAAARGASDLHLEPEAGFLRVRHRIDGTLRQVRA
                     MHKSCWAELAVRIKVLAGMDIAESRSPQDGRIGLALGGRPIDFRVATQPTLHGENIVL
                     RILDRDKGIVPLDALGLDPAQRAALDRILARPEGLILVVGPTGSGKTTTLYSMLGHLN
                     TEAVNIMTLEDPVEYPLALIRQTQVGDASRLDFADGVRALLRQDPDVILIGEIRDADT
                     ATMALRAALTGHQVFATLHANSIFGALPRLHEIGIGAELLAGNLCGILAQRLVRRLCP
                     TCREPVPDDAPERHLPGLAADAPPAPSWRARGCPECDFRGYRGRLAIMEILPFDAELD
                     ELVARRASPGELRAAARTRGHRSLAEDGLRRVLDGSTSLAELARVVDLSALAATAKTA
                     HGGEA"
     gene            8700..9902
                     /locus_tag="PKN25_06810"
     CDS             8700..9902
                     /locus_tag="PKN25_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV90643.1"
                     /translation="MSTWRYRALAADGRRVRGELDAADLGELERLLEARALLLIDAQP
                     RGVRGLLPRRRIARRELIPFCYHLEQLLAAGVPPFESLALLRDAGAEPRLQQVIGALI
                     ADVERGLPLSGAAARQPEAFSAVAVSLLRAGEQAGRLPEAIRDIGAALAQEEELASHA
                     RRIAIYPAIVATVLLAAVVVALIHVVPELERLLRGTGQRLPLQTRILVGLSAAVRDWG
                     WAMLLVTLAGAASSAHALARSEALRTRVHALLLQLPLVGGILRKLALARFAALFATLY
                     GAGINVIDALHTSTGATANLALRAGLREATGAIEQGRPISVAFEATRVFPPLVIRMLR
                     LGEHTGALDDALAKVASLYRRDVAEGIARLQAAAEPALTILMGGLLLWIASAVLGPIY
                     ELITRLPV"
     gene            9905..11407
                     /locus_tag="PKN25_06815"
     CDS             9905..11407
                     /locus_tag="PKN25_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90644.1"
                     /translation="MRREPLLLAVAPSGLLACIGNDDGITLLAAFTCGEEAAFASWLA
                     RRPPDEPCRMLVDLPDEAYQIEDLPRVRGSDRRALFARRLAHWFPEPRFARATPLGAL
                     PDGRQGAERVLFAGMERSTELLPWLDRLAADGRRPQILVPASALLPRLPLPGARQRRH
                     GKAPPRPRLLATHGRAGLRISLLAGEHTLFSRLVRGHADSLADPQALATELERTRDYL
                     AAQHRIASDAPLDRIVADLPAQTGTEHATLPPIGVAAADTLAGCGLPNSYDAHLLLAL
                     RHAPTTIGWPLAAGARRWPRLPERRVLGALALVASTILGALVWMEHQAQVEAAALAAT
                     ERARAARAAALAAEEAELAAREAERAALAALDAAPPPLPTPAPAPAEPAPAACPPASS
                     PPPGPIARRIDGVLRRPDGEILLWVEGTWQSARALGLHPIAGDAAVVVAAGRRTQLRS
                     GELVPTAVATVTAGPGQAYPERDDAAPADRAAGPADSAARPGVDMRGARP"
     gene            11485..12726
                     /locus_tag="PKN25_06820"
     CDS             11485..12726
                     /locus_tag="PKN25_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90645.1"
                     /translation="MRPRRPGAIRGQALIAALLVLTLLAAGGLLAGGELRARAHALAH
                     ARSVEVLATARAALIGYAISYAETHPGEGYGFLPCPDAGNTGSTPIGACGARGTAAIG
                     RLPWRTLGLPELRDGWGECLWYAVAGSVKHNPKPAALNWDSPGQFGLLDPAGRSLQAA
                     SGDGMAVAIVFSPGPPRSSQIRPPTRGTSCTGSDSAAADLSHYLDPGHARAGSGVIEI
                     TLHPATLDDEAPNDLATWIGIDDVFDALRRRRDHASHLDALLARSANALAGRLAASRT
                     EWLARHARPLGTLAVGMLPTAETLGIAAAQRPLIDDWHEQIRFAACPDGSACIGVTRP
                     AAPGERCRAVLAFGGERIRSGPERQLRTTAAERADPAAWFEGRNVAALRLGNPEFEGA
                     AHFRVTAPDRPASEDVVRCLP"
     gene            12714..13424
                     /locus_tag="PKN25_06825"
     CDS             12714..13424
                     /locus_tag="PKN25_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV90646.1"
                     /translation="MPALTPPPARMHKPAHSSAPARGFTLVELAMVLVILALLGGSLL
                     VPVASRLEARDRNATLERLHDIQLALTGFAIIHGRLPCPSTETDPASPGYGLEDGPPC
                     NLAREGVLPWRSLAMPATDAWGRDRHSAADDWTGYWRYRVDPAFSVAPIGAATTPGAR
                     LQIRDHDGRRITTTDSQAVAIVWSTGANLHADGDNTSHASATPSYQAGEPGADFDDLL
                     AWLGRPLLIARLAQAGRL"
     gene            13490..14131
                     /locus_tag="PKN25_06830"
     CDS             13490..14131
                     /locus_tag="PKN25_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV90647.1"
                     /translation="MQKKPQTGFTLVEIAIVLLVVGLLLGGVMKGQALIDSARVKNLA
                     QDLRSVPAMVHAYQDRFRALPGDDPAAVRHLCSGGSSCTVAGNGNGVIDGTWDAGADT
                     ESVRLWQHLRLAGIATGSTDTADAGFLPRNALGGRLGVQRGGSVLGLPGSLVVCSDNV
                     PGKLVTQIDIALDDGDPAAGSLRAGTPGDTGLVAISAANRLLEANAHTVCSNL"
     gene            complement(14160..16127)
                     /locus_tag="PKN25_06835"
     CDS             complement(14160..16127)
                     /locus_tag="PKN25_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HNV90648.1"
                     /translation="MLNTLEFVVLLLAAAVVMVALFRSMNLPPVLAYLLVGALGGPHA
                     LDLMQESAGARHLAEFGVVFLMFSIGLEFSLGRLMAMKRIVFGLGLAQVLTTIALATL
                     LGRLGGLGWITSFALGGTLAMSSTAILSKLLADRMELDSRHGREVIGVLLFQDIAVVP
                     LLILLPALSRPAEEMMFTLGLAAFKVVFLLSLVLVFGQRLMRWWFTVMARRRSGELFM
                     LNVLLITLGLAWLSERVGLSLALGAFLAGMLISETEYRYQVEEDIKPFRDVLLGLFFV
                     TVGMLLDVAEIVRNLPAVLGLLGALLVLKFGLVFAASRVFGSPAGTAMRSGLWLCAGG
                     EFGFVLLSQILDLGLMPYGLAQAAVAALVLSMLVAPLIVQVSDRLVMRFVASEWLLRS
                     MELTRVAAQSLNTERHIIVCGYGRSGQYLARFLEQENVAYVALDLDPERVREAGAAGD
                     TVVYGDAARRETLVAAGIQRASALVVSFDALEAALRVIHHAHALRPGLPVVVRAGDEA
                     DLEKLAQAGAAEVVPEAFEASIMLASHALALTGVPLSRVVRRIREIRSQRYALMRGFF
                     QGSSDAGDAGAGAEARLRSVIVPEGAAGVGRSIGELGLEEFGVAVSAVRRRGIRALSP
                     GAETRIQSGDVIVLLGVPGGLEAAEERLLGG"
     gene            16281..17270
                     /locus_tag="PKN25_06840"
     CDS             16281..17270
                     /locus_tag="PKN25_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNV90649.1"
                     /translation="MDPISASGESRAVALARRVLRIEADAVAALGARIGEEFERAVEI
                     ILARHGRVIVTGVGKSGHIGRKLAATLASTGTPAYFVHAAEAAHGDLGMITPEDVVIA
                     LSNSGSSEELLTIVPLVKRQGARLIAMTGKPDSPLAREADAHLDAGVAEEACPLNLAP
                     TASTTAALALGDALAVALLDARGFAAEDFARSHPGGALGRRLLTHVGDVMRPAPAVPR
                     VGSDAPLTQALLAMTAGGMGMTAVVDADEVPVGIFTDGDLRRALEKGCDVRSARVSEV
                     MTRSPRSIAPGALAAEAAATMENMRISQLLVLDDAGRLAGALTTHDLMLAKVI"
     gene            17271..17852
                     /gene="lptC"
                     /locus_tag="PKN25_06845"
     CDS             17271..17852
                     /gene="lptC"
                     /locus_tag="PKN25_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNV90650.1"
                     /translation="MRVDTWRLYPVFALALLAGGSVWLERITRIDDPAASIEQTGPDF
                     IAHDTRVVGFGADGAQRYELVAERLEHFPVGDITRLHTPRLQLHGAEGTTTITARAAD
                     VSPGGEQVDLTGEVRVRRPGQAGALPLALDSETLAVWPDAQRARTDSPVLMTRGAGRA
                     TAKGMRADNLFGTLELIGEVSTTMPPRRQGSPS"
     gene            17849..18391
                     /gene="lptA"
                     /locus_tag="PKN25_06850"
     CDS             17849..18391
                     /gene="lptA"
                     /locus_tag="PKN25_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HNV90651.1"
                     /translation="MKRILLATVAALLAAVTTPALAEKADRDQPVNIEADRLTVDDRN
                     KVHIFEGSVVLTQGTLTIRGDKLVVTQDAAGFQNGVATAAGKGLATFRQRRDGSAEYV
                     EGEAERIEYDSRTEKARLFNRAKVSSGGDQVSGHYIEYDAITENYLATNAPGSRQGAP
                     AGRVRAVIQPKSDGQPSGGQ"
     gene            18429..19205
                     /locus_tag="PKN25_06855"
     CDS             18429..19205
                     /locus_tag="PKN25_06855"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNV90652.1"
                     /translation="MSFEMILAEKRGRVGLITLNRPKALNALNDQVVNEITAALNDFE
                     SDDGIGAIVITGSEKAFAAGADIGAMANFSYMDAYKSEYITRNWERVKTCRKPVIAAV
                     AGFALGGGSELAMMCDIIIAADTAKFGQPEVKLGTLPGAGGTQRLPRAVGKAKAMELC
                     LTGRMMDAVEAERAGLVARVVPADKLLEDALATAETIAGYSLPVVMMIKESINRAFES
                     SLNEGLLFERRVFHSAFALNDQKEGMAAFVEKRKANFRHD"
     gene            19534..20100
                     /locus_tag="PKN25_06860"
     CDS             19534..20100
                     /locus_tag="PKN25_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNV90653.1"
                     /translation="MSNFVTPEQFTAANKANIETLLTLANTAFASAERLAALNLNTAR
                     SLLEDGVANTKALLAAKDVQELVNLQVSLAQPIVEKAVAYARSVYEISSQSQEEMSKV
                     FEGQVAELNKGVATALDKAAKSAPAGSDVAVAAVKSAIAAANSAYDSMSKAAKQVAEI
                     AEANVAAATNATVKAVSTTAKTTAKKAA"
     gene            complement(20212..20586)
                     /locus_tag="PKN25_06865"
     CDS             complement(20212..20586)
                     /locus_tag="PKN25_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNV90654.1"
                     /translation="MGEEIRALGEDEILASLAAGLPHWRLEAGALVRDYRTGGWKATL
                     MVAGVVGHLAEAAWHHPELELCWDRVRVRLSTHSPRGITARDFALARKIEEVVHWQPA
                     REGGALEGTPNADDRFRYIRYD"
     gene            complement(20651..21427)
                     /locus_tag="PKN25_06870"
     CDS             complement(20651..21427)
                     /locus_tag="PKN25_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV90655.1"
                     /translation="MHLLRSILFALVLTVITVPYAFFATLIFWLPPMTRHRLITSWVP
                     IMMWVIRHVLGIRYRVIGAENIPAGPAVVLSKHQSAWETIALQVIFPPLCYVLKRELL
                     RVPFFGWALGRIPGIAIDRSAGKDALTQVVEQGRERLKEGLWVVVFPEGTRVAPGATL
                     RYKPGGAILAKRAGVPVVPVAHNAGEFWRRNAFIKRPGEIVVSIGPVIDVKGAKATDV
                     NEQAEAWIEGEMRRLFPHLYREEAGMPPAAGDAAAEDEGN"
     gene            complement(21486..22019)
                     /gene="gmhB"
                     /locus_tag="PKN25_06875"
     CDS             complement(21486..22019)
                     /gene="gmhB"
                     /locus_tag="PKN25_06875"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HNV90656.1"
                     /translation="MKLIILDRDGVINVDSGQYIKSPEEWKPIPGALEAIARLNQWGW
                     RVVVATNQSGIGRGLFGMDTLNAINDRMVRSLAQAGGRLDGIFFCPHAADSTCDCRKP
                     KPGMLLQIAERFNVELADVPCVGDGLRDLQAAAAAGAQPWLVLTGKGEATRASGELPP
                     GTLVYPDLDAVAAALTA"
     gene            complement(22078..>22842)
                     /locus_tag="PKN25_06880"
     CDS             complement(22078..>22842)
                     /locus_tag="PKN25_06880"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNV90657.1"
                     /translation="RFAGDALPAGNVAAAVALADKLDALVGFFGIGMVPTGDKDPFAL
                     RRAALGVLRILMEAPLPLELPRLIDTAAAGFKPGLLTAEGFAAQLQDFMYERLKNLLR
                     ETGRDGAVIDAVLALRPARIDLVPAKLAAVEAFRALPEAEALAAANKRIVNILKKAEG
                     APGEPDLALLQEAAEKALFRQVVEVAPLVRSHVANEDYTDALRALAGLRAAVDQFFED
                     VMVMAEEPLTRANRIALLAQLAGLMNQVADISRLSA"
BASE COUNT         3561 a         8652 c         7603 g         3026 t
ORIGIN      
        1 ttgaagcctt ccatggatcc tcagttctga ttcatcacgc cgacgaagac ctgttccagg
       61 tccggcttgc cgatcgccat gtcgacgatt tccgccccgg cggatttcag gccggcgagg
      121 agctggccga cgtcgtcgaa gcgctcgaag gcgagttcaa gatcgacgcc gtcgcgcttg
      181 accacctggg cggccagcgc ctcgggcaaa acgacggcgg cggcgaggcg cacctgcaaa
      241 ttatggctgg cgaagcgctg gagcagattg gcggtggtat cgagggccac cacccgtccg
      301 gccttgagca tggcgatgcg cccgcacagg gactcggcct cctcgaggta gtgggtggtc
      361 agcacgatgg tatggccgtc ctggttgagc ttgcggatga actgccagag accatgacgc
      421 agctccacgt ccaccccggc ggtgggctcg tcgaggacga tgaccggggg gcgatgcacc
      481 agggcctggg ccaccagcgc ccggcgtttc atgcccccgg acagcatgcg catgttggcg
      541 ccggccttgc cggtgaggtc caggttatgg aggatctcgt cgatccagtc gtcgttgttg
      601 cggatgccga agtagcccga ctggatgcgc agcagctcgc gcaccgagaa gaagggatcg
      661 aagacgagct cctgcggcac cacgccgagg ttgcggcggg cgttgcgata gtcggcgacg
      721 acgtcgtgcc ccatgatcgc gagcgtcccg ctgtcgggcc gcaccagacc ggagagtgcc
      781 gagatcagcg tggtcttgcc cgcaccgttg gggccgagca ggccgaagaa ctcgccctgg
      841 ccgacctcga ggtcgacccc gccgagcgcc ttcaggctgc cgtagtgctt gcttacaccc
      901 tggatacgga tcgccggcgt gctcatgcgg cgcgctccag cggcagcagg ccgtcgatat
      961 cgtagagcgc agccaggctg gtcagcccgg caggcgggtt gcgcagcatc agcgcgtggc
     1021 cggccgcacg cgcgcagcgc atccagtcga ggatcagcgc gagcgccgcg gagtccgcgg
     1081 cggaaaccgc cgcgagatcg accaccaggt cgcccgcgcg cgcgcgccgc tgcccctcgg
     1141 cgagcagcgc cggcgcgctc tgcatggtca gcgcgcccgc gggcgcgaac agcgcgggat
     1201 cgctcacgcg ccgcgccccg cgtcgccctg gctcatgccc tgctgctcgg cgagcgcgcg
     1261 gttgcgctcc tcgagcgagc ggatcaggcc atcgatgcca ccgcgcgcaa tctcctgtcc
     1321 gaagctgctg cggtagttgg tgaccaggct gacgccggcg acgatcacgt cgaacacctt
     1381 ccagccctgt tcgcccttct ccagcatgta gtcgatgccg atcggttgcg caccggcctg
     1441 caccacctcg gtgcggacct gcaccctcgt gtctgcaggg gcgaagcgcg agggacggaa
     1501 ctcgatgcgc tggtcgcggt actgcgtcag cgcgtttgaa taggtacgca ccagcagggt
     1561 gcggaaggca tcggtcagcc tggtctgttg ctcggcgctg gcctggcgcc agtcgcgccc
     1621 gaccgcgagc atggtcatgc ggcggaagtc gaagtgcggc agcaccttgg cgtcgaccag
     1681 cgcgatcacg cgctgggtgt cgccggcctg gatcgccttg tcggcgcgca cgatctcgag
     1741 cacttcggtg gtgacgtcgc gcacgagcac atcgggcgcc ttgtcggcgc tggccgtcgc
     1801 gggcagcgcg gcgccgagca aggcgcaaag aaggaaggcg cggaggaaat cgaggatctt
     1861 gttcatggtt ctcgcttact ggacggattg gcgggcgacg cggcggccgg ccgtggccgg
     1921 gctcgcaccg atgtccgcgt gttcgaggtt cccgaccgca gaggccgcgg ccgtatcgac
     1981 gtcgccaccg ggcggtgcgg ccgactgttc gtcgaaatct tcgtacacgc gttcctggcg
     2041 gccatcgctg accttgtaac ggcgttgttc gagatagaag tcgcgcacga agacatagcg
     2101 gtcgagcgcc gcctcctcga gcgtgcggtc ggcaccgagc agcgtggagc ggaggtgcac
     2161 aacgcgcgtg cccagcacgg tgttgcgcgt cgccacgtga tcgatgctga aaggctcgtt
     2221 ggcgatgaag tcggcgggca gcgcgaccgc atcccgcacg gtccgcggac cgaagaaggg
     2281 cagcacgatg taggcgccct cgcccacgcc ccaacggccg agcgtctgcc cgaggtcctc
     2341 gtcgtgcttg ggcaggcccg cctcaccggc gacgtcgagc aggccgagca ggccccaggt
     2401 ggaattgagc gcgaagcgca tcaaatcgcc catgccctcg gcgaccttgc cctgcagcag
     2461 gttgttgagc gcgacccagg gatcgccgag gttgcctagc acgttgccga caccggtacg
     2521 caccggatgc ggcagcaccg cctcgtagac ctgggctgcg ggcttgatca ccaccttgtc
     2581 gacctgttcg ttgaagccga acatcgcgcg gttgtagccc tcgagtggat catcgggatt
     2641 cgcggtggtc gcacagccgg tcgcgagcgc ggccgacagc gcggccgcga ccaggcctgc
     2701 acgggaggag cggaagacgt ttcgcattgc tgttttctcc ggagttcctt gccgacctac
     2761 tgggcgggcg cgtcggcggc gcggtcgaac atgaactggc cgatgagttt ctccagcacc
     2821 accgcggact gcgtgatcag cacgcggtcg ccacccttga gcatcgcggt gtcggcaccg
     2881 gcctccagcc ccacgtactg ctcgccgagc aggccggagg tgaggatgga cgcggtggtg
     2941 tcggcgggaa acgcatagcg cttgtcgatc tcgagctcga ccaccgcgcg gtagatcgcg
     3001 ggatcgaagc ggatcaccgc gacccggccc acgaggacgc cggagctctt gaccggcgcg
     3061 cgcaccttga gcccgccgat gttgtcgaag ggggcctcga cgcgataggt ctcggcgccg
     3121 tcgaggccgg agaaattgcc gaccttcatg gcgaggaaca ccagcgcggc aaagccgatc
     3181 gccacgaaga ggccgaccca caggtcgagc gcggtacgac tcatcatcaa cccctgaaca
     3241 tgaaggaggt aagcacgaaa tccagcgcca ggatcgccag cgcggaggtc accacggtgc
     3301 gggtgatcgc gcgcgacacg ccctcggcgg tcggctggca atcgtagccc tcgaacaccg
     3361 cgatcagcga caccacggtg ccgaacaccg cggacttgat cacgccgttg acgacgtcgt
     3421 agcggaactc gaccgcggcc tgcatctgcg accagaacgc gccgtcatcg acgccgatca
     3481 cgaccacggt gatcacccag ccgccgaaca cgcccatcgc cgagaacagc gccgccagca
     3541 gcggcatcga cagcacgcca ccccagaagc gcggcgcgac cacgcgcgcg atcgggttga
     3601 ccgccatcat gtccatcgcc ttcaactgct cggtggtctt catcaggccg atctcggcgg
     3661 tgatcgccga gccggcgcgg ctggcgaaca gcagcgcggc gatcaccgga ccgagctcgc
     3721 gcagcagcga cagcgccacc atcaccccca ccgcgtcggc cgagccgtag cgctgcagga
     3781 tgtcgtagcc ctgcagcccg agcacgagac cgacgaagag ccccgagacc atgatgatca
     3841 gcagcgacag cacgccgttg aaatagagct cgcggatggt gagatggatg cgccgcagcg
     3901 actgcccgga gttcagcagc accgcgaaca ggaagcgggt ggcgaagccc aggcgccaga
     3961 tgccatcgat ggtcagcgca ccgatgcggc gcagcgcgga ggcgatcgcg ctcatgcgcc
     4021 ctccccgccg agcaggcgct cgatcggctc ggccggatag tggaagggca ccggcccgtc
     4081 ggcctcggcg tggacgaact gatgcacgaa gggatccctc gaggcgcgga tctcgtccgg
     4141 cgtgccctgc gccacgatgc ggccgtcgga gacgaagtag atgtagtcca cgatcgccag
     4201 cgactcgtgc acgtcgtggg tcaccatcac cgagctcgcg ccgagcgcgt cgttgagctt
     4261 gcggatgagc tggccgatga tgccgagcga gatcgggtcg aggccggcga agggctcgtc
     4321 gtacaggatc agcatcgggt cgagcgccac cgcgcgcgcc agcgccaccc gccgcgccat
     4381 gccgcccgag agctcgttgg gatgcagctt cgccgcgccg cgcagcccca ccgcctgcag
     4441 cttcatcagc accaggtcgc gcaccatgcc cgcgggcagg tcggtgtgct cgcgcagcgg
     4501 gaaggccacg ttgtcgaaca cgctcatgtc ggtgaacagc gcgccgaact ggaacagcat
     4561 gcccatgcgc cgacgcagcg cgtagagctc gctgcccgac agccgcgcca cgtcgcgccc
     4621 ctccacctcc acggtgccgc cggtcgcgcg cagttgcccg ccgatcaggc gcagcagcgt
     4681 ggtcttgccg cagccgctgc cgcccatgat ggcgaccacc tggccgcgat gcacgctcag
     4741 gtcgatgccg cgcagcacct cgcgctcgcc ataggcgaag cgcacgtcgc gcagcgaaac
     4801 cagcggggag ggatgtgggg cggacacggt cacgtcgatc ggccgaatgt gaaaactctt
     4861 gaatatatca gagatttgcg cccgctggcg gactgatgct gcagcgcagc aagcaggtcc
     4921 gggccattcc cccgcaggac acctcctccg ctccggtgac gaccgcggca cccagaaaat
     4981 aattccatgc gcttattcac attttatttc aatacgaata gcatgttcgc atgacatgca
     5041 catcgaccga cctgcgcagg ctgctcgcga ccgcggcgct tctggcgacc gctgcatgtg
     5101 cgccgctcgg ccgcccgccc gcactcgagg ggcatctcga caagcgcacc gcgatcgagc
     5161 gcaccgcgcc tgtcggcacg gcgacaagaa gcctcgcgcc gccgatcgcg gagtcgcctt
     5221 cttccagccg aggcgctgca gcgagcgcgg cgacgggcgc gcctccgccc gctccggccc
     5281 gcgggcgagc agctcccgcg gtgcgcggat actcgatcga cgttttcgac atcccggccg
     5341 agcagctgct gctcgccatc ggccgcgacg ccgggctgga gctcgacctc catgccggaa
     5401 tcaccggaaa cgtcagcctg caggcccacg accgcccgct cggcgagctg ctggagcgca
     5461 tcgcccgcca ggtcgacctg cgctatgagc tgcgcggccg ccacctgcgc gtgcgccccg
     5521 acacccccta catccgcagc tatgcggtgg actatgtgaa cctgagccgc gacatgcgcg
     5581 gcaccgtcgc caccagcacg cagatcgcca cctcgggcag cgccggcggc gccggtcagc
     5641 gcgcgtcttc gacaggcggc gctggcaacg cctcgatcac ccagatcgag acgcgctcgg
     5701 ccaacgactt ctggaacacg ctcgagcaca acctcgccgc catcctcgcc gggccggggc
     5761 gcgcgcccga ctgcgggggc gagcgccgcg gcgagtcccc cgccggctgc cgcaacgccg
     5821 acctgatgct caaccgcgag agcggcatcg tcatggcgcg cgccacccag cgccagcacg
     5881 agcagctgga ggccttcctc gcgggggtgc agcgcgcggc gcggcgccag gtgatgatcg
     5941 aagccacgat cgtcgaggtc accctcgccg acggctaccg ccagggcatc gactggaccc
     6001 ggctcggcgt gccgagctgg agcgtgcgcc cgcgcggcag cggcgacgcc acacccggcc
     6061 agcccacgct gagctacctg tcggccaact ccgacatccg tctcgaactg ctggagacct
     6121 tcggcaccgt caaggtgctc tccagcccgc gcctgtcggt gctcaacaac cagaccgcga
     6181 tgctcaaggt cgtcgaggag gtcgtctact tcctcgtaga cagcaccacc accgagtacg
     6241 acgaccgcaa gcaggcccgc atctccgcga ccaccacgcc gcaatcggtc tcggtcggca
     6301 tggtcatgtc ggtcacgcca cagatctccg ccgacggcga catcaccctc aacgtgcggc
     6361 cgaccatctc gggcatctcc ggcttcaagg acgaccccaa cccctcgctc ggcgacatcc
     6421 ccaaccgcgt gccgcagatc cgcacacgcg aaatcgagtc catgctgcgc ctgcgcagcg
     6481 gggaggtcgc cgtcctcggc gggctgatgg aggaccgcat cgaccacgac accggccgca
     6541 tcccgctgtt cggcgacctg cccgtggtcg gcgaactatt cacccgccgc gacaacaacg
     6601 tgcgccgcac cgagctgctc atcttcctgc gccccctgct gatcgacgag ccgggcatgc
     6661 gcggcggcta cgccgaatac gccggccacc tgcccgacga cgacttcctc ctcgactcgc
     6721 cctcgcccgg ccagcgcaac ttcccccacc tgccgctgcg cccgctgctc gcacccgcag
     6781 gcgcggtctc cgccgccgaa ggcttttcgg aagcggccta tcccgaccct gccgatcccc
     6841 gctctcctgc cgcctcctcc acgtccgcca gcgccgccaa cgcggatgcc ggcacgagcc
     6901 cgccatgaac gctccgctcc ccatcggcca gatcctcatc gccgccggcc tgatcggcga
     6961 ggaccagctc cgcatcgcgc tccacgagca acgcggccgc gcccggccgc tcggccgcgt
     7021 gctggtcgaa ctcggcttcg tcagcgaggc cgcgctgcgc gaggcgctcg ccgcacgcag
     7081 cggcctgccc tgcgtcgacc tggccagcgc gctcgccgac cccgacgcga tcgcgcgcgt
     7141 gccgcaggcg ctcgcaaggc gccaccgcct gctgccgctc cagtacgacg ccgcccgcca
     7201 ccgcctgatc gtcgcgatgg ccgacgccca cgacatcgtc gccctcgacc gtctgcgtgc
     7261 cgagctcggg ccggacgtgc acgtcgaact gcgcctggcc ggcgacaacg aactcggccg
     7321 cgcgatcgaa cagcactacg gtcaggcgag ctcgatcgag gacatggtgc gcgagctcga
     7381 acgccgcgcc ggacagccca tcgctggcgc gcgcgacccg caactcgtcg tgcgcctggt
     7441 cgacgccctg ctcgccgagg ccgccgcgcg cggcgcctcc gacctgcacc tggagcccga
     7501 ggccggcttc ctgcgcgtgc gccaccgcat cgacggcacg ctgcgccagg tgcgcgcgat
     7561 gcacaagtcg tgctgggcgg agctcgccgt gcgcatcaag gtgctcgccg gcatggacat
     7621 cgccgagtcg cgcagcccgc aggacgggcg catcggcctt gcgctcggcg gccggccgat
     7681 cgacttccgc gtcgccaccc agcccacctt gcacggcgag aacatcgtgc tgcgcatcct
     7741 cgaccgcgac aagggcatcg tcccgctcga cgcgctcggc ctcgaccccg cgcaacgcgc
     7801 cgccctcgac cgcatcctcg cccgccccga gggcctgatc ctggtggtcg gtcccaccgg
     7861 cagcggcaag accacgacgc tgtactccat gctcggccac ctcaacaccg aggcggtcaa
     7921 catcatgact ctggaagatc cggtcgagta tccgctcgcg ctgatccgcc agacccaggt
     7981 cggcgacgcc tcacggctcg acttcgccga cggcgtgcgc gcgctgctgc gccaggaccc
     8041 cgacgtgatc ctgatcggcg agatccgcga cgccgacacc gccaccatgg ccttgcgcgc
     8101 cgcactcacc ggccaccagg tgttcgccac cctgcacgcc aactcgatct tcggcgcgct
     8161 gccgcgcctg cacgagatcg gcatcggcgc cgaactgctc gccggcaacc tgtgcggcat
     8221 cctcgcccag cgcctggtgc gccggctgtg ccccacctgc cgcgagccgg tgccggacga
     8281 cgcccccgag cgtcacctgc ccggcctcgc cgccgatgcc ccgccagcgc cgagctggcg
     8341 cgcgcgcggc tgccccgaat gcgacttccg cggctatcgc ggccgtctcg cgatcatgga
     8401 gatcctgccc ttcgacgccg agctcgacga gctcgtcgca cgccgcgcca gcccgggcga
     8461 gctgcgtgca gccgcacgca cgcgcggcca tcgcagtctg gccgaggacg gactgcgccg
     8521 ggtgctcgac ggcagcacca gcctcgccga gctcgcccgc gtggtcgacc tctccgcgct
     8581 cgcggctacg gcgaagaccg cgcacggagg tgaggcatga gcgtgcccct gcggccgccc
     8641 gaagggcagc gcgctcgtcc gccgcggagg atgtcgtgca gcgacgggag gaaggcccca
     8701 tgagcacctg gcgctaccgc gcgctcgctg ccgacggccg ccgcgtgcgc ggcgagctcg
     8761 atgcggccga cctcggcgaa ctcgagcgcc tgctcgaagc gcgcgccctg ctgctgatcg
     8821 acgcccaacc acgcggcgtg cgcggcctgc tgccgcgccg gcgcatcgcg cgccgcgagc
     8881 tgattccctt ctgctaccac ctcgagcaac tcctcgccgc cggcgtgccg cccttcgaga
     8941 gcctggcctt gctgcgcgac gccggcgccg agccgcggct gcagcaggtg atcggcgcac
     9001 tgatcgcgga cgtcgagcgc ggcctgccgc tgtcgggcgc cgccgctcgc cagcccgagg
     9061 ccttctccgc ggtcgcggtg agcctgctgc gcgcgggcga gcaggccggc cgcctgcccg
     9121 aggcgatccg cgacatcggc gccgcgctcg cgcaggaaga agaactcgcc agccacgcgc
     9181 gccgcatcgc gatctatccc gccatcgtcg ccacggtgct gctggccgcg gtggtggtcg
     9241 ccctcatcca cgtggtgccc gaactggagc gtctgctgcg cggcaccggc cagcgcctgc
     9301 cgctgcagac gcgcattctg gtgggactgt ccgcggcggt gcgcgactgg ggctgggcca
     9361 tgctgctcgt cacgctcgcc ggcgcggcga gcagcgccca cgcgctcgcc cgctccgaag
     9421 cgctgcgcac ccgcgtgcac gccctgctgt tgcaactgcc gctggtcggc gggatcctgc
     9481 gaaagctcgc cctcgcgcgc ttcgccgcgc tcttcgccac cctctacggc gccggcatca
     9541 acgtcatcga cgcgctccac accagcaccg gcgccaccgc caacctcgcc ctgcgcgccg
     9601 gactgcgcga ggccacgggc gcgatcgagc agggccgccc gatctcggtc gccttcgagg
     9661 cgacccgggt cttcccgccc ctcgtcatcc gcatgctgcg cctgggcgaa cacaccggcg
     9721 cgctcgacga cgcgctcgcc aaggtcgcca gcctctaccg ccgcgacgtc gccgagggca
     9781 tcgcccgcct gcaggccgcg gccgaacccg cactcaccat cctcatgggc ggcctgctgc
     9841 tgtggatcgc gagcgcggtc ctggggccga tctacgagct catcacccgc ctgccggtct
     9901 gaccatgcgt cgcgaacccc tgctcctcgc cgtggctccg tccggcctcc tcgcatgcat
     9961 cgggaatgac gacggcatca ccctcctcgc tgccttcacc tgcggcgagg aggccgcctt
    10021 cgcaagctgg ctcgcccgcc gcccgccgga cgagccctgc cggatgttgg tcgacctgcc
    10081 cgacgaggcc taccagatcg aggacctgcc gcgcgtgcgc ggcagcgacc gtcgcgccct
    10141 gttcgcgcgc cgactcgctc actggttccc cgagccgcgc ttcgcacgcg ccacgccgct
    10201 cggcgccctg cccgacggac gccagggcgc cgagcgggtg cttttcgccg ggatggagcg
    10261 cagcaccgaa ctcctgccct ggctggatcg cctcgcggcc gacggacgcc gcccccaaat
    10321 cctcgtcccc gccagcgccc tcctgccacg cctgcccctg ccaggagcac gccagcgccg
    10381 ccacggcaag gcgccgccac ggccgcgcct gctcgccacc cacgggcgcg cgggcctgcg
    10441 catttcgctg ctggccggcg aacacaccct gttctcccgc ctcgtgcgcg gccacgccga
    10501 ctccctcgcc gatccgcaag cgctggcgac ggagctcgag cgcacccgcg actacctggc
    10561 cgcccagcac cgcatcgcca gcgatgcgcc actcgatcgc atcgtcgcgg accttccggc
    10621 tcagaccggg acggagcacg ccacactgcc tccgatcggc gtcgccgccg ccgacacgct
    10681 cgcgggctgc ggactcccga acagctacga cgcccatttg ctgctcgccc tgcgccacgc
    10741 ccccacgacg atcggctggc cgctcgcggc cggcgcacgg cgctggccca ggctgcccga
    10801 aaggcgcgtg ctcggcgcgc tcgcgctcgt cgcctcgacg atcctgggag ccctcgtctg
    10861 gatggagcac caggcgcagg tcgaggccgc agccctggcc gcgaccgagc gcgcccgtgc
    10921 ggcgcgtgcc gccgcgcttg cggccgagga ggcggaactc gccgcacgcg aggccgaacg
    10981 cgccgcactc gccgccctcg atgcagcgcc gccgcccctt ccgaccccag cgccggcccc
    11041 cgccgagcct gcaccggcag cctgtccgcc tgcctccagc ccgccgcccg ggcccatcgc
    11101 caggcgcatc gacggcgtcc tgcgtcgccc ggatggcgag atcctgctct gggtggaggg
    11161 aacctggcag tccgcgcgcg cactcggcct gcacccgatc gcaggcgacg ccgcggtggt
    11221 agtggcggcc ggccggcgca cgcagctacg cagcggagag ctcgtgccga ccgccgtcgc
    11281 gacggtcacg gccgggcctg ggcaggccta ccccgagcgc gacgatgcgg cacccgcaga
    11341 tcgagcagcg ggccccgccg acagcgctgc aaggcccggt gtcgacatgc gcggagcgcg
    11401 gccatgatcc ggagggcccg cctgccctgc cgggagcggc gccgccagcg ggatccgcac
    11461 tccgctcgcg tgcccgcgcg ggtgatgcgt ccccggcgac ccggcgcgat ccgcggtcag
    11521 gccctgatcg ccgccctgct ggtgctcacc ctgctcgcgg cgggcggcct gctcgccggc
    11581 ggcgaactgc gcgcccgcgc ccacgcgctc gcccacgcgc gcagcgtcga ggtgctcgcc
    11641 accgcacgcg ccgcgctgat cggctacgcg atcagctacg ccgagaccca ccccggagag
    11701 ggctacggtt tcctgccctg tccggatgcc ggcaacaccg gctccacccc gatcggtgcc
    11761 tgcggtgcgc gcggcaccgc cgcgatcggg cgcctgccct ggcgcaccct cggcctgccc
    11821 gagctgcgcg acggctgggg cgaatgcctg tggtacgcgg tcgccggcag cgtcaagcac
    11881 aaccccaagc cggcggcgtt gaactgggac agcccgggcc agttcggcct gctcgatccc
    11941 gccgggcgca gccttcaggc agcgtccggc gacggcatgg cggtggcgat cgtgttcagc
    12001 cccgggccgc cacgcagcag ccagatccgt ccgcccaccc ggggcacgag ctgcacaggc
    12061 agcgactcgg ccgcagccga cctttcgcac tacctcgacc ccggccatgc gcgcgcaggc
    12121 agcggggtga tcgagatcac cctccacccg gccaccctcg acgacgaggc gcccaacgac
    12181 ctcgccacct ggatcggaat cgacgacgtc ttcgacgcac tgcgtcggcg ccgcgaccac
    12241 gccagccatc tcgatgcgct cctcgcccgc agcgcgaacg cgcttgccgg ccggcttgcg
    12301 gcgagccgca ccgaatggct cgcacgccac gcccgcccgc tcggcacgct cgcggtgggt
    12361 atgctgccca cggcggagac cctgggcatc gccgccgcac aacgtccgct catcgacgac
    12421 tggcacgagc agatccgctt cgccgcctgc cccgacggca gcgcctgcat cggtgtgacg
    12481 cggcccgcgg cacccggcga gcgctgccgg gcggtgctgg cattcggggg cgagcgcatc
    12541 cgcagcggcc ccgagcgcca actgcgaacc accgctgccg agcgcgccga tccggcggcc
    12601 tggttcgagg gccggaacgt cgccgcgcta cgcctgggca atcccgagtt cgagggtgcc
    12661 gcgcacttcc gcgtcaccgc gcccgatcgc cccgccagcg aggacgttgt ccgatgcctg
    12721 ccctgacccc gcctcccgct cgcatgcaca agcccgcgca ttcgagcgct cccgcgcgcg
    12781 gcttcacgct ggtcgagctc gccatggtgc tggtgatcct cgccctgctt ggcggcagcc
    12841 tgctggtccc ggtggcgagc cggctggaag cgcgcgaccg caacgccacg ctcgagcgtc
    12901 tgcacgacat ccagctcgcg cttaccggct tcgccatcat ccacggccgc ctgccctgcc
    12961 cgagcaccga aaccgatccc gccagccctg gctacgggct cgaggacggc ccgccctgca
    13021 acctcgcgcg cgagggcgtg ctgccctggc gcagcctggc gatgccggcg accgacgcct
    13081 ggggccgcga ccgccactcc gccgccgacg actggacggg gtactggcgc taccgcgtcg
    13141 accccgcctt cagcgtggcg ccgatcggcg ccgccaccac acccggcgca cgcctgcaga
    13201 tccgcgatca cgacggccgg cgcatcacca cgaccgactc gcaggcggtc gcgatcgtgt
    13261 ggtcgaccgg cgccaacctg cacgccgacg gcgacaacac cagccacgcc agcgccaccc
    13321 cgagctacca ggccggcgaa ccgggcgccg atttcgacga tctgctcgcc tggctcgggc
    13381 gcccgctgct gatcgcccgt ctcgcccagg ccgggcggct gtgatcccgg ccgcgcatcc
    13441 gcacaaagcc ctttccatca ccactgccac atcacccggg agacaaacca tgcaaaagaa
    13501 accgcaaacc ggattcaccc tcgtcgagat cgcgatcgtg ctgctcgtcg tcggcctgct
    13561 gctcggcggg gtcatgaagg ggcaggcgct gatcgacagt gcccgtgtca agaacctcgc
    13621 ccaggacctg cgcagcgtgc cggcgatggt ccatgcctat caggaccgct tccgcgccct
    13681 ccccggcgac gaccccgccg ccgtccgcca tctctgctcc ggcggcagca gctgcaccgt
    13741 ggccggcaac ggcaacggcg tgatcgacgg cacctgggat gcgggcgccg acaccgagtc
    13801 cgtccgcctc tggcagcacc tgcgcctggc cggcatcgcc accggcagca ccgacaccgc
    13861 cgacgccggc ttcctgccgc gcaacgcact cggtggccgg ctcggcgtgc agcgcggtgg
    13921 cagcgtgctc ggcctgccgg gcagcctggt ggtgtgctcg gacaacgtgc ccggcaagct
    13981 ggtcacacag atcgacatcg cgctcgacga cggcgacccc gcggccggct cgctgcgtgc
    14041 cggcacaccc ggcgacaccg ggctggtggc gatctccgcg gccaaccggc tgctcgaagc
    14101 gaacgcgcac acggtgtgtt cgaatctgtg aggcgtgagg cgtgcggagc gcgggcggct
    14161 cagccaccca gcaggcgctc ctcggcggct tccaggccgc cggggacgcc gagcagcacg
    14221 atcacgtcac cgctctggat gcgtgtctcg gcacccggcg agagcgcgcg gatgccgcgc
    14281 cgacgcactg ccgacaccgc cacgccgaac tcctccagcc cgagttcgcc gatgctgcgc
    14341 ccgactcccg ccgcgccctc cgggacgatc accgagcgca ggcgcgcctc cgcgccggcg
    14401 cccgcatcgc ccgcatcgct gctgccctgg aagaagccgc gcatcagcgc atagcgctgg
    14461 ctgcggatct cgcggatgcg ccgcaccacg cgactgagcg gcaccccggt gagcgcgagc
    14521 gcgtgcgagg cgagcatgat gctggcctcg aaggcctcgg gcaccacctc ggccgccccc
    14581 gcctgggcaa gcttctccag gtcggcctcg tcgccggcgc gcaccaccac cggcaggccg
    14641 ggacgcagcg cgtgggcgtg atggatcacc cgcagcgccg cctccagcgc gtcgaaggac
    14701 accaccagcg cgctcgcgcg ctggatgccg gcggcgacca gggtctcgcg ccgcgcggcg
    14761 tcgccgtaca ccacggtgtc cccggccgcg cccgcctcgc gcacgcgctc cgggtcgagg
    14821 tcgagcgcga cgtaggcgac gttctcctgt tcgaggaagc gcgccaggta ctggccgctg
    14881 cgtccgtagc cgcacacgat gatgtggcgc tcggtgttga gcgactgcgc cgccacccgg
    14941 gtgagctcca tcgagcgcag caaccattcc gaggcgacga agcgcatgac caggcggtcg
    15001 ctcacctgca cgatcagcgg cgcgaccagc atcgacagca ccagcgcggc caccgcggcc
    15061 tgggcgaggc cgtagggcat cagcccgagg tcgaggatct gcgacagcag gacgaaaccg
    15121 aactcgccgc ccgcgcacaa ccacagcccg gagcgcatcg ccgtgcccgc cggcgaaccg
    15181 aacacgcgcg aggccgcgaa caccagcccg aacttgagca ccagcagcgc gccgagcagg
    15241 ccgagcactg ccggcaggtt gcgcacgatc tcggcgacgt cgagcaacat gccgacggtg
    15301 acgaagaaga gcccgagcag cacgtcgcgg aagggcttga tgtcctcttc cacctggtag
    15361 cggtactcgg tctccgagat cagcatgccg gcaaggaagg cgccgagcgc gagcgacagc
    15421 ccgacccgct cggacagcca ggccagcccg agcgtgatca gcagcacgtt gagcatgaag
    15481 agctcgcccg agcgccgccg cgccatcacg gtgaaccacc agcgcatcag gcgctggccg
    15541 aagaccagca ccagcgagag caggaagacc accttgaagg ccgccaggcc gagggtgaac
    15601 atcatctcct cggccgggcg cgacagcgcc ggcagcagga tcagcagcgg caccaccgcg
    15661 atgtcctgga acagcagcac gccgatcacc tcgcggccat gacggctgtc gagctccatg
    15721 cggtcggcga gcagcttgga gaggatcgcg gtggacgaca tcgccagcgt gccgccgagc
    15781 gcgaagctgg tgatccagcc cagcccgccg aggcgaccga gcagggtggc gagcgcgatc
    15841 gtcgtcagca cctgggcgag tccgagcccg aacacgatgc gcttcatcgc catcaggcgc
    15901 ccgagcgaga actccagccc gatcgagaac atcaggaaca ccacgccgaa ctcggcgagg
    15961 tggcgcgcgc cggcggactc ctgcatcagg tcgagcgcat gcggcccgcc gagcgcgccg
    16021 acgagcaggt aggcgagcac cggcggcagg ttcatgctgc ggaagagggc caccatcacc
    16081 acggcggcgg ccagcagcag gaccacgaat tcgagggtat tcagcatgga agcccggccg
    16141 cgtgtggcgc cagcgctgcc gggcggcggc gcgggtgttt ggtatacaat ccgctcgatc
    16201 gggagcttgc cggcaacggc gcgccgtcat cccgtcgagc caccggtctc ccttccgcgc
    16261 cagtttaaag catcgaatcc atggacccaa tttccgcttc gggcgaaagc cgagccgtcg
    16321 ccctcgcccg ccgcgtgctg cgcatcgagg ccgacgccgt ggccgcgctc ggggcgcgca
    16381 tcggcgagga attcgagcgc gcggtcgaga tcatcctcgc gcgccatggc cgggtgatcg
    16441 tcaccggcgt tggcaagtcg ggccacatcg gccgcaagct cgccgccacg ctcgccagca
    16501 ccggcacccc ggcctacttc gtgcatgccg ccgaggccgc acacggcgat ctcggcatga
    16561 tcaccccgga agacgtggtg atcgcactgt cgaactccgg ctccagcgag gagctgctca
    16621 ccatcgtgcc cctggtcaag cgccagggcg cccgcctgat cgcgatgacc ggcaagccgg
    16681 actcgccgct cgcacgcgag gccgatgcgc atctggacgc cggcgtggcc gaggaggcct
    16741 gcccgctcaa cctcgcgccc accgcgagca ccacggcggc gctcgcgctc ggcgacgcgc
    16801 tcgcggtcgc gctgctcgac gcccgcggct tcgccgccga ggatttcgcc cgctcccacc
    16861 ccggcggcgc gctcggccgc cggctgctga cgcacgtcgg cgacgtcatg cgtcccgcgc
    16921 cggcggtccc gcgcgtgggc agcgacgccc ccctcaccca ggccttgctg gcgatgaccg
    16981 ccggcggcat gggcatgacc gcggtggtcg acgccgacga ggtgcccgtg ggcatcttca
    17041 ccgacggcga cctgcggcgc gcgctggaga agggctgcga cgtgcgcagc gcgcgcgtca
    17101 gcgaggtcat gacgcgcagt ccgcgcagca tcgcgcccgg cgcgctcgcg gccgaggccg
    17161 ccgcgacgat ggagaacatg cgcatcagcc agctcctggt gctcgacgac gccggccggc
    17221 tcgccggtgc gctcaccacc cacgacctga tgctcgcgaa ggtcatctga atgcgggtcg
    17281 acacctggcg cctgtatccg gtcttcgccc tcgcgctgct ggcgggcgga tcggtgtggc
    17341 tcgagcgcat cacccggatc gacgatccgg cggcgagcat cgagcagacc ggtccggact
    17401 tcatcgccca cgacacccgc gtggtcggct tcggcgccga cggtgcgcag cgctacgagc
    17461 tggtggccga acgcctggag catttccccg tcggcgacat cacccggctg cacacgcccc
    17521 gcctgcagct gcacggcgcg gaaggcacga ccacgatcac cgcgcgcgcg gccgacgtct
    17581 cgcccggtgg cgagcaggtg gacctcaccg gcgaagtgcg cgtgcggcgt ccgggccagg
    17641 ccggggcgct gccgctcgcg ctcgactcgg agacgctcgc ggtgtggccg gacgcccagc
    17701 gcgcgcgcac cgactcgccg gtcctgatga cccgcggcgc cgggcgcgcg acggccaagg
    17761 gcatgcgcgc ggacaacctg ttcggcacgc tcgaactcat cggcgaggtc agcacgacga
    17821 tgccaccccg ccgccaagga tccccctcat gaagcgaatc ctcctcgcta ccgtggccgc
    17881 gctgctcgcc gccgtcacca ccccggcgct cgccgagaag gccgaccgcg accagccggt
    17941 caacatcgag gccgaccgcc tcacggtgga cgaccgcaac aaggtgcaca tcttcgaggg
    18001 cagcgtggtc ctgacccagg gcacgctcac catccgcggc gacaagctgg tcgtgacgca
    18061 ggacgccgcc ggcttccaga acggcgtcgc caccgcagcc ggcaagggct tggccacctt
    18121 ccgccagcgc cgcgacggca gcgccgaata cgtcgaaggc gaggccgaac gcatcgagta
    18181 cgacagccgg accgagaagg ccaggctctt caaccgcgcc aaggtgagca gcggcggcga
    18241 ccaggtcagc ggccactaca tcgagtacga cgcgatcacc gagaactatc tcgccaccaa
    18301 cgccccgggc agccgccagg gtgcgcccgc gggccgggtg cgcgcggtga tccagcccaa
    18361 gtccgacgga cagccctccg gcggacaatg attccccccc acgatcggat aaccaggaag
    18421 gagacagcat gagcttcgag atgatcctcg ccgagaaacg cggccgtgtc ggcctcatca
    18481 ccctcaaccg ccccaaggcg ctcaacgcgc tcaacgatca ggtggtgaac gagatcaccg
    18541 ccgccctcaa cgacttcgag tccgacgatg gcatcggtgc catcgtcatc accggctcgg
    18601 agaaggcctt cgccgcaggc gccgacatcg gcgcgatggc gaacttctcc tacatggatg
    18661 cctacaagag cgagtacatc acgcgcaact gggagcgcgt gaagacctgc cgcaagccgg
    18721 tgatcgccgc ggtggcgggc tttgcgctcg gcggcggcag cgagctggca atgatgtgcg
    18781 acatcatcat cgccgcggac accgcgaagt tcggccagcc ggaagtcaag ctcggcaccc
    18841 tgcccggcgc gggcggcacg cagcgcctgc cgcgcgcggt gggcaaggcg aaggcgatgg
    18901 agctgtgcct gaccggacgc atgatggacg cggtcgaagc cgagcgcgcc ggcctggtcg
    18961 cgcgcgtggt gccggccgac aagctgctcg aggacgcgct cgccaccgcg gaaaccatcg
    19021 ccggctactc gctgccggtg gtcatgatga tcaaggaatc gatcaaccgc gccttcgaga
    19081 gcagcctcaa cgagggcctg ctgttcgagc gccgcgtctt ccactccgcg ttcgcgctca
    19141 acgaccagaa ggaaggcatg gcggccttcg tcgagaagcg caaggcgaac ttccgccacg
    19201 actgatcgtc ccgcgctcga tccgcaccgc ggccggcgtc cagccggccg cttgcgttta
    19261 cccgcgggaa gacgtcatgc gcctgcaacc cgtgcccggc acactgcttc ggcgtactcg
    19321 ccgcccgtgc aatgcaacac cccgaaccct tcaccggacg aaatggcgtc ctggcgcaag
    19381 tgattgattc cttcaatcaa tccgggatgt tgcgatgcac caaaatatgc ttgacagcct
    19441 cgatcgggtc cgtataatcc gaaccatgct gcaacgcaac atcgcagcta cccgagcgat
    19501 gttgatgcac ccacactttc caaggagatc cacatgagca acttcgtcac ccccgagcag
    19561 ttcaccgccg ccaacaaggc caacatcgag accctgctga ccctggccaa caccgccttc
    19621 gccagcgccg agcgcctcgc cgccctgaac ctgaacaccg cccgttcgct gctggaagac
    19681 ggcgtggcca acaccaaggc cctgctggct gccaaggacg tgcaagagct ggtgaacctg
    19741 caggtttcgc tggcccagcc gatcgtcgag aaggccgtcg cctacgcccg cagcgtctac
    19801 gagatctcgt cgcagagcca ggaagagatg tcgaaggtct tcgaaggcca ggtcgccgag
    19861 ctcaacaagg gcgtcgccac cgcgctcgac aaggctgcca agtcggctcc cgccggttcg
    19921 gacgtcgccg tcgccgctgt caagtcggcc atcgccgccg ccaacagcgc ctacgacagc
    19981 atgagcaagg ccgccaagca ggtcgccgag atcgccgaag ccaacgtggc tgccgcgacc
    20041 aacgccaccg tcaaggccgt cagcaccacc gccaagacca ccgccaagaa ggctgcctaa
    20101 gcttcctccg gcaccgtctg acaaaaaggc cccgcatgcg gggccttttt gcgtctaccg
    20161 gctggtgacg cggcagggcg cggactgcag cggaaacgca cgcacgccgc ttcagtcgta
    20221 gcggatgtaa cggaagcggt cgtccgcgtt cggcgtgcct tccagcgccc cgccctcgcg
    20281 cgcgggctgc cagtgcacga cctcctcgat cttgcgcgcg agcgcgaaat cgcgtgcggt
    20341 gatgccgcgc ggcgaatgcg tggacaggcg cacccgcacc cggtcccagc acagctccag
    20401 ctcgggatga tgccaagccg cctcggccag gtgcccgacc acaccggcca ccatcagcgt
    20461 cgccttccag ccgccggtgc ggtagtcgcg caccagggcg ccggcctcga ggcgccagtg
    20521 cggcagcccc gccgcaaggc tggcgaggat ctcgtcctcg ccgagtgcgc ggatttcttc
    20581 acccatcgtc cctctcccga tgtctggggc cgccccggcg gcctatgcgg gagaccgggc
    20641 gagaacgcgc tcagttcccc tcgtcttccg ccgcggcatc gcccgcggcc ggcggcatgc
    20701 ccgcctcttc gcgatagaga tgcgggaaca gccggcgcat ctcgccttcg atccaggcct
    20761 ccgcctgctc gttcacatcg gtggccttgg cacccttgac gtcgatcacc gggccgatgc
    20821 tgacgacgat ctcgcccggg cgcttgatga aggcgttgcg cctccagaac tcgcccgcgt
    20881 tgtgcgccac cggcaccacg gggacgcccg cgcgcttggc gaggatggcc ccgccgggct
    20941 tgtagcgcag cgtcgcaccc ggcgccaccc gagtgccctc ggggaagacc accacccaca
    21001 ggccctcctt gaggcgctcg cggccctgct ccaccacctg ggtgagcgcg tccttgcccg
    21061 cggagcggtc aatggcgatg cccgggatcc tgcccagcgc ccagccgaag aagggcacgc
    21121 gcagcagctc gcgcttgagc acgtaacaca gcggcggaaa gatcacctgc agcgcgatcg
    21181 tctcccatgc cgactggtgc ttggacagca ccaccgcggg accggccggg atgttctcgg
    21241 cgccgatcac gcggtagcgg atgccgagca cgtggcggat cacccacatc atgatcggca
    21301 cccagctcgt gatcagccgg tgccgggtca tcggcggcag ccagaagatg agggtggcga
    21361 agaacgcgta gggcaccgtg atcacggtaa gcacgagtgc gaacaggatg gagcggagca
    21421 gatgcacgat tctcgtccgg tgaagagtgc gtgagggatg cggcagcgct gtcgcgccgc
    21481 gccgctcagg cggtcagcgc ggcggcgacc gcatcgaggt cgggatacac cagcgtaccg
    21541 ggcggaagct cgccgctcgc gcgcgtggct tcgccctttc cggtgagcac cagccagggc
    21601 tgcgcgccag ccgccgcggc ggcctggagg tcgcgcaggc cgtcgccgac gcagggcacg
    21661 tcggcgagct cgacgttgaa gcgctcggcg atctgcagca gcatgcccgg cttgggcttg
    21721 cggcagtcgc aggtggagtc ggccgcgtgc ggacagaaga agatgccgtc gagccgcccg
    21781 cccgcctggg cgaggctcct gaccatgcgg tcgttgatgg cgttgagcgt gtccatgccg
    21841 aacagcccac ggccgatgcc ggactgattg gtcgccacca ccacccgcca gccccactgg
    21901 ttgagccgtg cgatcgcctc cagcgcgccg gggatcggct tccactcctc gggcgacttg
    21961 atgtactgcc ccgagtcgac gttgatcacg ccgtcgcggt cgagaatgat gagcttcatc
    22021 acgggctcct tgcgggggcg aagcccgcgg cgacgcacgc cgcgggggct atgacgatca
    22081 ggccgacagg cgcgagatgt ccgccacctg gttcatcagg ccggcgagct gggcaagcag
    22141 cgcgatgcgg ttggcacggg tcagcggctc ctccgccatc accatgacgt cttcgaagaa
    22201 ctggtccacc gctgcgcgca gtccggcgag tgcacgcagc gcatccgtgt agtcctcgtt
    22261 ggcgacgtgc gaacgcacca gcggcgccac ctcgaccacc tgacggaaga gcgccttctc
    22321 ggcagcttcc tgcagcagcg cgaggtcggg ctcgcccggc gcgccttcgg ccttcttgag
    22381 gatgttgacg atgcgcttgt tggccgcggc cagggcctcg gcctcgggca gcgcgcggaa
    22441 ggcctccacc gcggcgagct tggccggcac caggtcgatg cgcgcgggtc tcagcgccag
    22501 cacggcgtcg atcacggcgc cgtcacggcc cgtctcccgt agcaggttct tcagacgctc
    22561 gtacatgaag tcctgcagct gcgcggcgaa gccttcggcc gtcagcaggc cgggcttgaa
    22621 gccggccgcc gcggtgtcga tcagcctggg cagctcgagc ggcagcggcg cttccatgag
    22681 gatgcgcagc acgccgagcg cggcgcggcg cagcgcgaac ggatccttgt cgccggtggg
    22741 caccatgccg ataccgaaga agccgaccag ggcgtcgagc ttgtcggcca gcgccaccgc
    22801 ggcggcgacg ttgccggcgg gcagcgcgtc gccggcgaag cg
//
LOCUS       DAOQBS010000043        22518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1521_length_22518_cov_16.618973, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000043 DAOQBS010000000
VERSION     DAOQBS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 22518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22518
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1521_length_22518_cov_16.618973"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..3854
                     /gene="recC"
                     /locus_tag="PKN25_06885"
     CDS             210..3854
                     /gene="recC"
                     /locus_tag="PKN25_06885"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNV90658.1"
                     /translation="MTAPAPAASLPPGFIALHGNRIETLLDTVAGWLAQHPLAPLESE
                     VILVQSNGMAEWVKMAIARRAGVCAAAAVQLPARFQWQTYRQVLGRAQVPARSPLDKT
                     ALAWRLMRLLPEVLAEPGFAPVAGYLADNDPGRRFQLALRIADLYDQYQVHRPDWLDA
                     WGEGREALPDAGRGETPLTGRGETPLAGRGETPLPEDQRWQARLWQRVVDELDPQQRA
                     LTRPQLHDRVLAALDAIAEDAALHPAAALPRRVVLFGMTHVPLPTLQLLAALSRHAQV
                     LLAIPNPCRFHWADILDGREWLRIARRRQPLRDGRELQDVGLDAMHLHAHPLLAAWGR
                     QARDFVRQLDAFDDAEQTRARFPLARIDLFDDDQDTHDVAQPTLLVQVQRHIRDLTPL
                     AEHPHPPVPAEDRSIVFHLAHSPVRELEILHDQLLALLARPPGDKPLRPRDIVVMVPS
                     IDDFAPAIRAVFGQYGRHDPRHIPFDIADLSARASSPLMGAVEWLLRIRHDRCRLSDL
                     GALLDVPAIAARFGLAEDGLPRLTGWMRGAGIRWGLDARHRAGLGLQACGEQNSALFG
                     LRRMLMGYAAGRSEVTMDAPFAGIEPYDEVGGLDAELAGALAHLVERLIRWQADSRRP
                     ATPAAWTQRLRALLADLVRAVDDTDRQTLAALDDALERWLEACDEAAFEAELPLAVAA
                     EAWLAALESPALDKRFRAGGVTFCTLMPMRAIPFEVVCLLGMNDGDYPRRSPRSDFDL
                     MALPGQQRPGDRARRDDDRQLMLEALLSARRVLYLSWCGRNVRDNSEQPPSVLVAQLR
                     DYLAAGWSPAVVAERTVEHPLQPFSRRYFEPGTPLLTYAREWRAAHVEAAAQDDAVPS
                     HDGQANREATGRATRPTRGEAASPPLPPFVPDPAVPLTIAALAAFLRNPVKVFFRERL
                     GVVYEDVDEALPDDEAFGFDGLEEHGLVEELAAAVLADLALLPPPELHAFALEAAVEA
                     QLGRIERAGRLPIGGLGLRARAALGEAVLPLLRAWRLVRASHPLDAERRNLRFAHAGA
                     LMEDWLDHRVRADEHALPSWLAHDPGRLLEDADKGTLRPWRLLLPWVRSLLAAAADSP
                     SGGLLVGRDATVHIVPTDPAVARALLARLIEAWIAGLGEPLPVAARTALALVGEGKPA
                     EAYEGSEHRRGEVEEASLARCYPDYAALIEDGRFEHLARALYGPLLEWMDSHVEAVPH
                     PAPETQESAR"
     gene            3851..7816
                     /locus_tag="PKN25_06890"
     CDS             3851..7816
                     /locus_tag="PKN25_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNV90659.1"
                     /translation="MSHRLDPACFPLRGSRLIEASAGTGKTWTIAALYLRLVLGHGDA
                     DTAFARPLLPAEILVMTFTRAATRELSERIRERLREAAAWFRNEDAGRAPDPLLDELR
                     AAYPDAAARRNAAWRLAMAAAAMDDAAVFTIDAWCQRMLREHAFDSGNPFDEELAADE
                     SVLLTEAAQDYWRQHCYPLAGEALELVLAQWADVHALVEDMRALVGQPLQGADRDDGG
                     DRDRSADPGDQDGVRRDSAGGETLAALIGRVGGAHRRARVALSRGWAERARAMKDWLD
                     EELATKAKLWNRTKLHERYYTPWLEALEQWARNPLDRRLELPDSARHRLTPDGLLEAR
                     KADAPAPVLPEHFAAFAGLIEDHDALPDPRTALRIHAATRVGERLAELKRQARSFGFA
                     DLLDRLDRALAGPHGARLRERILTQYPVALIDEFQDTSPLQYRLFDALYRTADDDPRS
                     ALLLIGDPKQSIYGFRGADIYSYLQARRATAGRHYMLGTNFRSAHALVSAVNTWFAQA
                     EDRPGAGAFGFRDGADNALPFEAVAAQGREEVFHADGGVPPALLIHHDLTLRSAGEIR
                     RLFAARCAERIVGWLNDEHAGFQAPGKAFQRLRPADIAVLVRSGREAAEVRRALRRRG
                     LASVYLSDKDSVFASAEAHDLLHWLRAVASPLDARLVRAALATRTVGLSIPELARLAS
                     SDAAFDARSEQFRALHVAWRELGVLTMLRRSLHLLDLPARWLAEPEGERRLTNFLHLA
                     ELLQQAGATLEGETALVRWLAMTIEAGGSRAGEEQVVRLESDADLVKVVTVHKSKGLE
                     YPVVCLPFATSFRAVERRTARYLKLADASGRRRLALDYDDADLERAEAERLREDLRLF
                     YVALTRARHALWMGFAAVRIGKGKACQTHRSAPGHLLAGGAALDEAAWLAPLQALATA
                     AAQDGATGTEADGRTRIALEAAPPEEAHTLLRRREALPPLQDSAPYRADFERRWSLGS
                     FSALVRDIDPAQMVALPGAGSAPPAPARGTTADLFAAAPDEAGSAAPLAADDYGQAEA
                     AGAARILPVHVRRPAADEALVRLEEDAALAPSGADGGLALAPPDTDRPPWHRFPRGAF
                     AGDFLHDVLEWLAAEGFALATNEGLEAALRRRCERAGHGERADELVQWMRALLGARLP
                     SLDAALQDLRVLITEMEFWLPSAQLQATAVDALCRRHLLHAQPRPALPQRELHGMLMG
                     FADLVFEHDGRYWVLDYKSNSLGEDGSAYDRAALEAAMAHHRYDVQAAIYLLALHRLL
                     RARLGGAYEPARQLGGALYLFLRGIDGPARGEYAIPATPALIELLDTLDHLLADEASL
                     A"
     gene            7813..9864
                     /gene="recD"
                     /locus_tag="PKN25_06895"
     CDS             7813..9864
                     /gene="recD"
                     /locus_tag="PKN25_06895"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004319516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HNV90660.1"
                     /translation="MSPIHASPSPAAAPHAATAPSAPTDALLATLDAWTDAGLLRQLD
                     SAFARFIASLDPAAPPALLVGAAVLARMEGRGHSCLPLAPLAATDQDLLAWPMEAQAA
                     IAALWQNLPADSAAWLAALQQSPVVRRIGHDPELGQPLLVDGPDTAPRLYLRRYWQYE
                     RSVAAAVAARCAQPEPVDEARARTWLDRLFPAAPGTPAHEAAVDWQKLACAIALRGRL
                     SILTGGPGTGKTYTAARLLALMLATHPAPERLRIALAAPTGKAAARLRQAIDGSLQAL
                     QRSLGGHLDLAALTRRIGAARTLHALLGARPDTRRFRHHAGNPLDVDVVIVDEASMVH
                     LEMMAALLEALPRGARLVLLGDKDQLASVEAGAVLGDLCRDASDGRYDAETARYLLAT
                     TGEALPTRYLEPTDKAPALAQHTVMLRESRRFEGPIGALAHAVNAARDPALPARLLRE
                     DASGALHGVEGGPVAAVIALALGGRRGAPACYRDYLELLARGPAGKDAEAHAAWATAI
                     LHAFDRFRVLCAVREGDWGMAGLNRAIEHALARARLLSPRGTWYAGRPVMVTRNDPAL
                     GVFNGDIGITLPALESGGLRVHFLDGEALRSVAASRLAHVETAFAMTVHKSQGSEFEH
                     TVLVLAAQSGNVLVRELIYTAITRARKAFSLVAEREGLLEHGVLQATRRESGLLDFQG
                     T"
     assembly_gap    9998..10097
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10231..11997)
                     /locus_tag="PKN25_06900"
     CDS             complement(10231..11997)
                     /locus_tag="PKN25_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV90661.1"
                     /translation="MTDRVPMQADADILIVDDNPANVELLLALLEDAGYQRLEGITDP
                     RRVAARVAEHAPDLILLDVRMPHLNGLELMERLSAGGAEAPAVIILTAQIDDATRYRA
                     LELGARDFLTKPFDQVEVLQRIRNVLHMQRLMKERAERAELLEALVAERTQELVARAR
                     HDPLTGLPNRLSLLDELGAWLAARRDAAVLFLSIEGMDEVSRLHGFGVADELARAAAR
                     RLRECVPEGFLAVWNSTQWVLLCDCAPARGATGALAERLLAGFRPPVEVEHMRLHLGL
                     RIGIAAEADERSPEQLVRMAALALPAGEGQWQAYEAELETRMQRRNGIREALRGAVGR
                     DEFSLVYQPKVELCSGDIVGVEALLRWDSSAYGRVSPAEFVPIAEASGEILAIGGWVV
                     RAALATLRRWREARRVADVFGMAINVSTQQLMQPDFAERLVAEVEASGVPPAALELEV
                     TESGLMQDMALAARHLDHLARAGFRIAIDDFGTGHSSLAYLKALPVSVLKIDRAFIRE
                     LHANPADQRLTGTVIDMARHFEFITVAEGVEQEAQLERLMDMGCDLVQGFMFAPPLKE
                     DALFRLMKTGFAHMPVFGGAVA"
     gene            complement(11990..14626)
                     /locus_tag="PKN25_06905"
     CDS             complement(11990..14626)
                     /locus_tag="PKN25_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV90662.1"
                     /translation="MIDPGLPADEPERLAELRALAILDTPREDAFDNLMQLAASIFGV
                     PIALVSLVDEARQWFKSAHGLCVAQTPRDVSFCTHVVFSNAPLVVEDAARDPRFADNP
                     LVTAAPQVRFYAGEPLRSDQGFVLGTLCVIDTVPRRFEEAERARLRLLARQAEQLIRL
                     HRKRQRLADEARRSARIAARYQAITEGAAAGILRIDGRGRIIEVNRFACALFGYEEAE
                     LRGHNVRMLMPEPFRSAHDGHLEAYQRTGVARVIGKGTEVDALHRDGRHIPVQLTVSE
                     VSQGAQADDFEGRHFIGILSDLRDVHQVRERERQERALLEVLHRGLTDYHALISGNTL
                     WGFLKDALCALTGSAYGLIGEVEHGEGGPALKIHAITDLSWNDETRGLMRKLVSGDMR
                     LSNPASMLGRVFARGEVVLSNAMRSDPRGGAMPPGHPPLSRYLGVPIMDRGEVIGMFA
                     IANAPQDYPDELVDWLKPFTSTCALLINLYRLFKEQQRFTEELQQARDQAEKANQAKT
                     EFLSSMSHELRTPLNAILGFAQLLQNGRQPLSERQLRQVEQIARSGRHLLQLINEVLD
                     LARIESGRLQVSLEPMLAHDVIEDAAQIVLPLAEQQGIALALPPAQACALRFTGDYTR
                     VKQILINLLGNAIKYNRPGGAVEVRCEAVGGRCRIGVVDTGIGIPASRLGELFQPFNR
                     LGAESGSIEGTGVGLALTRKLVDLMHGELGVHSREGEGSAFWFELPLCEQAPAVRDEA
                     RAAAAPQALSRRHRVLHVEDNPANRQLMQDVFEDVGQAVLHSVDSAEAGIEAACSDPP
                     DLILMDIDLPGMSGLEARALLARNPLTAHIPVLAISAAASPHTVRKAREAGFIDYVTK
                     PFDVPALVARLGQILEHRSSDD"
     gene            14873..17017
                     /locus_tag="PKN25_06910"
     CDS             14873..17017
                     /locus_tag="PKN25_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR N-terminal domain-containing protein"
                     /protein_id="HNV90663.1"
                     /translation="MRSDELDFHALIDFMPDSGAIRFCGRRAILVHADSMGALRKELV
                     ETLGSEIAKVVLTRYGFSCGYEDAELLAAYMHPDSLEEFILGGPRTHMFSGIAEVAPD
                     ALELDHASGRYRMSGRWRHSYEAEQHLRLFGRADEPVCWTLTGYASGFASRVLQADMI
                     CVETACEGRGDPHCAWTLMNASDCAPELAGLREYFKPLNLRDRLNLLESRVHERTREL
                     EASEQRYRNLIEDLPEMAFALHASGRLLHLNKAARLRLGVSEARLHALRLKDLVLPDY
                     TARATAFLKEIAARRSTTTLDLVMRDAAGLPFPVRLQVEPVLKDDRIVGYSGLALDIS
                     AQLERERELTEYARRIESREQQIQDVINEAVYILDGQGRLSFVNTRMAELLGAAPEAA
                     IGHAVGDFMPASSAARIERDFARRIAGEAGRPFEIALSGREGSSVVLEVNSTLFVGRD
                     VAEGVIGVARDITARRQMERELAQANRLSALGQFASGVAHEINNPLGLVSGYAEELQA
                     LLDECAPLGDDRRLAQLRRGLATIQQQAHRCKAITANLLAFSRRQSASLETVDVGLFV
                     AERLAFFNDAGLTRGVELAVDIAPGLPQAGTSPALLEQVLQNLIKNACDAMNGSGRID
                     IAARAVGEGIEIEIGDSGPGFAPGVAERVFDPFFTTKPPGKGTGLGLSICYAIINELG
                     GRIACGNRPQGGAWFRLVLPLAEDGPGAAKHD"
     gene            17010..18407
                     /locus_tag="PKN25_06915"
     CDS             17010..18407
                     /locus_tag="PKN25_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004319523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV90664.1"
                     /translation="MTDRPITDRHVLIVDDEELYRELLGGRLGRQGYRISEAGDGEGA
                     LRCIEGGGVELALIDIKMPGMDGIELLERIKQLAPRTEVVVLTGHGSIDSAIAAMKLG
                     AFDYLTKPYQLTELDLVVARALERSALARRCDALSVEVARLRSRDDDGMVGDSPAWLR
                     MLEQVRRAAALDVPVLLTGESGAGKEVVAAALHRWSRRAGEAYVPVNCGLLEGELVES
                     ELFGHKRGAFTGASADREGLFELASAGTLFLDEIGELPLACQAKLLRVLDSGEFRPLG
                     ASAVRRTGARVIAATHRDLEALVAAGRFRHDLLYRLDVVHIVVPPLRERREDIPLLVE
                     HLLHQAVRRHPGTPAPRVEPQALQRLIDYAWPGNVRELRNVVERLLVFADGDRIDAAR
                     VDALLHPGARTVASTGRTGFERIVPLAEMEQAYLRWVVDKLDGNVSAAATRLGIARST
                     IYRLLRPHADEGARP"
     gene            18707..19066
                     /locus_tag="PKN25_06920"
     CDS             18707..19066
                     /locus_tag="PKN25_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNV90665.1"
                     /translation="MQSNKIRILVASLALSCAHIALAADPPAEGRDRTYAKVCSFCHD
                     KGVGPAILGRALPAAYIENVVRHGFRAMPSFRSSEIDNAALASVARWVSQSPASKAST
                     ASPASPAPSAAPATKAQ"
     gene            19076..19666
                     /locus_tag="PKN25_06925"
     CDS             19076..19666
                     /locus_tag="PKN25_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90666.1"
                     /translation="MDRREFFRRTGGGMLLGAPLASLPATAAALVSPAGAAAAVVVIG
                     QADRSGAVTLAQRLAAALGHGGRPLPVHARDAAALRRPEQVDALLALPHGTRLLGVMD
                     DASAIVFLAMASSRGAHLLAHGHHRLASQGATRHTCSATGCGEALAWHEPASHPATHV
                     ERFYLATLGETTPGARTSLPAVPGGDEALASFLIRL"
     gene            19722..21272
                     /locus_tag="PKN25_06930"
     CDS             19722..21272
                     /locus_tag="PKN25_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNV90667.1"
                     /translation="MTTTPRNVLPKGVSANDFEQALAQIRAIVGAEYVVAEDTALAPY
                     NKIMMAVPDEQHAPSAAVMPDSVEQIQAIMKVLNQHRIPVYPISTGKNLGYGSAAPVE
                     RGQLVMDLRRMNRILDVDAELCTALVEPGVTYQQLYDYLQEKKLPLWFSCPAPSVIAG
                     PLGNMVDRGVGYTPYGEHFLFSSGMEVVLADGEVLRTGMGSMPGSNTWQVFKWGYGPT
                     LDGIFTQSNYGIVTKMGMWLMPAPPDYRPFLIQYPEDTDITKIVDALRPLRIAMVIPN
                     AVVIAHTLWEAPCTKVNRADYHPGPGAISDEAVQKIRKDHNLGAWNVYAALYGTPETN
                     AANWKIIEQVAAATGGTLITDSTEQGGRATQYRFDLMKGKPNLGEFDLYNWRGGGGSM
                     WFAPVSQAKGSETLKQMQLAKGILAKYGFDYVGEFIVGWRDMHHVIDLLYDRSDAEQM
                     KKAHACFDELLQAFAREGYGVYRANTAFAEKVAATYGPVKRDVERRLKRALDPNNIIA
                     PGRCGISI"
     gene            21343..>22518
                     /locus_tag="PKN25_06935"
     CDS             21343..>22518
                     /locus_tag="PKN25_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNV90668.1"
                     /translation="MNEIHLLIGGERRRATDGASFERRNPLDHGVATRAPAATAADAV
                     AAVEAAAAAFPAWAATGPGERRALLMKAAHALEARAEAFTAAMAAETGASAIWAGFNV
                     HLAAGMLLEAAALTTRIEGSILPSDVPGSVAMAVRQPAGVVLGIAPWNAPVILGVRAI
                     ATPLACGNTVVLKGSELCPATHGLIIEALQDAGLPAGVVNFVTNAPADAGAVVEAMVA
                     HPALRRVNFTGSTHVGRLIAQTCARYLKPAVLELGGKAPFVVLDDADLDAAVAAATFG
                     AFANSGQICMSTERIVVDAAVADDFVARLAARARALPLGDPRKGPVVLGSVVDQRTVE
                     RCNALIDDALAKGATLVCGGKADSTLMPATLLDHVSAQMRIYHEETFAPVKAIVRVQG
                     "
BASE COUNT         3093 a         8619 c         7734 g         2972 t
ORIGIN      
        1 tctccgtagg agcgacgcga gtcgcgatca cggcgcttgc cgtcacgcca cgactctccg
       61 caggagcgac gcgagtcgcg atgcaccgga ctgcgccgcc cccagccggt ccggtgggac
      121 gaggcataat ctgcgcacga ctcgcggcgc atgcggacga atacgccaaa ccatcccgca
      181 cggctgcgca ctgaacgatg ccctccaaga tgaccgcccc tgcccccgcc gcctccctcc
      241 cccccggctt catcgccctg cacggcaacc gcatcgagac cctgctcgac accgtcgccg
      301 gctggctggc gcagcatccg ctcgcccccc tcgaatccga ggtgatcctg gtgcagagca
      361 acggcatggc ggaatgggtg aagatggcaa tcgcgcgccg ggccggggta tgcgcggcgg
      421 cggcggtgca actgccggcg cgcttccagt ggcagaccta ccgccaggtg ctcgggcgcg
      481 cgcaggtccc ggcgcgctcg ccgctcgaca agaccgcgct cgcctggcgg ctgatgcggc
      541 tgctgcccga ggtgctggcc gaacccggct tcgcgccggt cgccggctac ctcgcggata
      601 acgaccccgg ccgccgcttc cagctcgcgc tgcgcatcgc cgacctctac gaccagtacc
      661 aggtgcaccg gcccgactgg ctcgacgcct ggggagaagg gcgcgaggcg ctgcccgacg
      721 ccggccgcgg cgagactcca ctcaccggcc gcggcgagac tccactcgcc ggccgcggcg
      781 agaccccgct gcccgaggac cagcgctggc aggcacggct gtggcagcgc gtggtcgacg
      841 agctcgaccc gcagcagcgc gcgctgaccc ggccgcaact gcacgaccgc gtgctcgcgg
      901 cgctcgacgc gatcgcggag gacgccgccc tgcacccggc cgccgcgctg ccgcgccgcg
      961 tcgtgctctt cggcatgacc cacgtgccgc tgcccacgct gcagctgctc gccgcgctgt
     1021 cgcgccacgc ccaggtgctg ctcgccatcc ccaacccctg ccgcttccac tgggccgaca
     1081 tcctggacgg gcgcgagtgg ctgcgcatcg cccgccgccg ccagccgctg cgcgacggcc
     1141 gcgagctgca ggacgtcggg ctggacgcga tgcacctgca cgcccacccg ctcctcgccg
     1201 cctggggtcg ccaggcgcgc gacttcgtgc gccagctcga cgccttcgac gacgccgagc
     1261 agacgcgcgc gcgcttcccg ctcgcccgca tcgacctctt cgacgacgac caggacaccc
     1321 acgacgttgc ccagcccacc ctgctcgtcc aggtccagcg ccacatccgc gacctcaccc
     1381 cgctcgccga gcatccgcac ccgccggtgc cggccgagga ccgctccatc gtcttccacc
     1441 tcgcccacag cccggtgcgc gagctcgaga tcctccacga ccagctcctc gccctgctcg
     1501 cccggccccc cggggacaag ccgctgcgcc cgcgcgacat cgtcgtgatg gtgccctcga
     1561 tcgacgactt cgcgccggcg atccgcgcgg tcttcggcca gtacggccgc cacgacccgc
     1621 gccacatccc cttcgacatc gccgacctgt cggcgcgcgc gagcagcccg ctgatgggcg
     1681 cggtggagtg gctgctgcgc atccggcacg accgctgccg gctgtccgac ctcggcgccc
     1741 tgctcgacgt gcccgccatc gccgcccgct tcggccttgc cgaggatggg ctgccacggc
     1801 tcaccgggtg gatgcgcggc gccggcatcc gctggggcct cgacgcgcgt caccgcgcag
     1861 ggctcggcct gcaggcctgc ggcgagcaga acagcgcgct cttcggcctg cgccgcatgt
     1921 tgatgggcta cgccgccggg cgttcggagg tcacgatgga cgcgcctttc gccggcatcg
     1981 agccctacga cgaggtcggc ggcctcgacg ccgagctcgc cggcgccctc gcccacctgg
     2041 tcgagcgcct gatccgctgg caggccgaca gccgccgacc cgccacgccc gcggcctgga
     2101 cccagcgcct gcgcgccctg ctcgccgacc tggtgcgtgc ggtggacgac accgaccgcc
     2161 agaccctggc cgcgctcgac gacgccctcg aacgctggct cgaggcctgc gacgaggccg
     2221 ctttcgaggc cgagctgccg ctcgccgtcg ccgccgaggc ctggctcgcc gcgctcgaga
     2281 gccccgcgct cgacaagcgc ttccgcgccg gcggggtcac cttctgcacg ctgatgccga
     2341 tgcgtgcgat ccccttcgag gtggtctgcc tgctcggcat gaacgacggc gactatccgc
     2401 gccgcagccc gcgcagcgac ttcgacctca tggccctgcc gggccagcag cgcccgggcg
     2461 accgcgcccg ccgcgacgac gaccgccagc tcatgctcga ggccctgctc tcggcccgcc
     2521 gggtgctcta cctgagctgg tgcgggcgca acgtgcgcga caacagcgag cagccgccct
     2581 cggtgctggt cgcccagctg cgcgactacc tcgccgcggg ctggtcaccc gcggtggtgg
     2641 ccgaacgcac cgtcgagcac ccgctgcagc ccttcagccg gcgctacttc gagccgggca
     2701 cgcccttgct cacctacgcg cgcgagtggc gcgccgcgca cgtcgaggcg gcagcgcagg
     2761 acgatgccgt gccgtcgcat gacgggcagg cgaaccgcga agccacgggc cgcgcaactc
     2821 gtcccacgcg cggcgaggct gcctccccgc cgctgccccc cttcgtcccc gacccggcgg
     2881 tcccgctcac catcgcggcg ctcgccgcct tcctgcgcaa cccggtcaag gtcttcttcc
     2941 gcgagcgcct cggcgtggtg tacgaggacg tggacgaggc cctgcccgac gacgaggcct
     3001 tcggcttcga cggtctcgag gaacacggcc tggtcgagga actcgccgcc gcggtactcg
     3061 ccgatctcgc cctgctgcca ccacccgagc tgcacgcctt cgcactcgaa gccgcggtcg
     3121 aggcccagct cggacgcatc gagcgcgccg gccgactgcc gatcggcggc ctcggcctgc
     3181 gcgcccgcgc cgcactcggc gaggccgtgc tgccgctgct gcgcgcgtgg cggctggtcc
     3241 gcgccagcca tcccctcgac gccgagcgcc gcaacctgcg cttcgcgcat gccggcgcgc
     3301 tcatggagga ctggctggac caccgcgtgc gcgccgacga gcacgcgcta ccctcgtggc
     3361 tcgcccacga ccctggccgc ctgctcgaag acgccgacaa gggcacgctg cgcccttggc
     3421 gcctgctgct gccctgggtg cgcagcctgc tcgccgccgc ggcggacagc ccgagcggcg
     3481 gcctgctggt cggccgcgac gccaccgtcc acatcgtccc caccgacccc gccgtcgcgc
     3541 gcgccctgct cgcccgcctg atcgaggcct ggatcgcggg cctgggcgag ccgctgccgg
     3601 tggcggcccg caccgcgctc gccctcgtcg gcgagggcaa gccggccgaa gcctacgagg
     3661 gcagcgagca ccggcgcggc gaggtcgagg aggccagcct cgcacgctgc tatcccgact
     3721 acgccgcgct gatcgaggac ggccgcttcg agcacctcgc gcgcgcgctc tacggcccgc
     3781 tgctcgaatg gatggacagc cacgtcgagg ccgtccccca cccggcaccc gagacccagg
     3841 agtctgcccg atgagccacc gcctcgaccc cgcctgcttt ccgctgcgcg gcagccgcct
     3901 catcgaggcc agcgccggaa ccggcaagac ctggaccatc gccgcgctct acctgcgcct
     3961 ggtgctcggc cacggcgacg ccgacaccgc cttcgcgcgt ccgctgctgc ccgccgagat
     4021 cctggtgatg accttcaccc gcgccgccac gcgcgagctc tccgagcgca tccgcgagcg
     4081 cctgcgcgag gctgcggcct ggttccgcaa cgaagatgcg ggccgcgcac ccgaccccct
     4141 gctcgacgaa ctgcgcgctg cctaccccga cgcggccgcg cgccgcaacg ccgcctggcg
     4201 cctggcgatg gcggccgcgg ccatggacga cgcggcggtg ttcaccatcg acgcctggtg
     4261 ccaacgcatg ctgcgcgaac acgccttcga cagcggcaac cccttcgacg aggagctcgc
     4321 cgccgacgag agcgtgctgc tgaccgaggc cgcgcaggac tactggcgcc agcactgcta
     4381 cccgctcgcc ggcgaggcgc tggagctcgt actcgcgcag tgggccgacg tgcacgccct
     4441 cgtcgaggac atgcgcgcgc tcgtcggcca accgctgcag ggcgcggatc gggatgacgg
     4501 cggggaccgg gatcggagcg cggatcccgg cgaccaggac ggcgtgcggc gcgattcggc
     4561 cggtggcgaa accctggccg cgctgatcgg gcgcgtcggc ggcgcgcacc gccgggcgcg
     4621 ggtggcgctc tcgcgtggct gggccgagcg cgcgcgcgcc atgaaggact ggctggacga
     4681 ggaactcgcc accaaggcca agctgtggaa tcgcaccaag ctgcacgagc gctattacac
     4741 gccctggctg gaggcgctcg aacagtgggc gcgcaacccg ctcgaccgca ggctcgagct
     4801 ccccgacagc gcccgccacc gcctcacccc agacggcctg ctcgaggcgc gcaaggccga
     4861 cgccccggcg cccgtgctgc ccgagcactt cgccgccttc gccggcctga tcgaagacca
     4921 cgacgcccta cccgacccgc gcaccgcgct acgcatccac gccgccaccc gggtcggcga
     4981 gcgcctcgcc gagctcaagc gccaggcgcg cagcttcggc ttcgccgacc tgctcgaccg
     5041 tctcgaccgc gccctcgccg ggccgcacgg cgcgcgcctg cgcgagcgca tcctcaccca
     5101 gtacccggtg gcgctgatcg acgagttcca ggacacctcg ccgctgcagt accgcctctt
     5161 cgacgcgctc taccgcaccg ccgacgacga cccgcgcagc gcgctgctgc tgatcggcga
     5221 ccccaagcag tcgatctacg gatttcgcgg cgcggacatc tacagctacc ttcaggcccg
     5281 ccgcgccacc gccggccggc actacatgct cggcaccaac ttccgctctg cgcacgcgct
     5341 ggtgagcgcg gtcaacacct ggttcgccca ggccgaggac cgcccgggcg cgggcgcctt
     5401 cggctttcgc gacggggcgg acaacgccct gcccttcgag gcggtggcgg cgcaggggcg
     5461 cgaagaggtc ttccatgccg atggcggcgt gcccccggcg ctgctcatcc accatgacct
     5521 gacgctgcgc agcgccggcg agatccgccg cctgttcgcc gcgcgctgcg ccgagcgcat
     5581 cgtcggctgg ctcaacgacg agcacgccgg cttccaggcc cccggcaagg cattccagcg
     5641 cctgcgcccg gccgacatcg cggtgctcgt gcgcagcggg cgcgaggccg cggaggtgcg
     5701 gcgcgcgctg cgccggcgcg gcctggcctc ggtctatctg tcggacaagg actcggtctt
     5761 cgccagcgcg gaggcgcacg acctgctgca ctggctgcgc gcggtggcct cgccgctcga
     5821 cgcccgcctc gtgcgcgccg cgctcgccac ccgcacggtg ggcctgtcga tccccgaact
     5881 ggcccggctg gcgagctcgg acgcggcctt cgacgcgcgc agcgagcagt tccgcgcgct
     5941 gcacgtcgcc tggcgcgagc tcggcgtgct caccatgctg cgccgcagcc tgcacctgct
     6001 cgacctgccc gcgcgctggc tggccgagcc cgagggcgag cgccggctca ccaacttcct
     6061 gcacctggcc gagctgctgc agcaggccgg cgccacgctc gagggcgaga ccgcgctggt
     6121 gcgctggctg gcgatgacga tcgaggccgg cggcagccgc gccggcgagg aacaggtggt
     6181 acgcctggag agcgacgccg acctcgtcaa ggtggtcacg gtgcataaga gcaagggtct
     6241 cgaatacccc gtggtctgcc tccccttcgc caccagcttc cgcgcggtgg agcgcaggac
     6301 cgcgcgctac ctcaagctcg ccgacgccag cgggcggcgg cggctcgcgc tcgactacga
     6361 cgacgccgac ctcgaacgcg ccgaggccga gcgcctgcgc gaagacctgc gcctgttcta
     6421 cgtcgcgctg acgcgcgcgc gccacgcgct gtggatgggc ttcgccgcgg tcaggatcgg
     6481 caagggcaag gcctgccaga cccatcgcag cgcgcccggg cacctgctcg cgggcggcgc
     6541 tgcgctcgac gaggccgcct ggctcgcccc gctgcaggcg ctggcgacag ccgccgcgca
     6601 ggatggcgcg accggcacgg aggccgacgg ccgcacccgc atcgcgctcg aggccgcgcc
     6661 gcccgaagag gcgcacaccc tgctgcgccg gcgcgaggcc ttgccgccgc tgcaggacag
     6721 cgcgccctac cgcgccgact tcgagcgtcg ctggtcgctc ggcagcttct ccgcgctggt
     6781 gcgcgacatc gatccggcgc agatggtcgc cctgccgggc gccggaagcg cgccaccggc
     6841 gcccgcacgc ggcaccaccg ccgacctctt cgccgcggcg cccgatgaag ccggctcggc
     6901 agcccccctg gcggcggacg attacggcca ggccgaggcg gccggggccg cccgcatcct
     6961 gccggtccac gtccgccgcc cggcggccga cgaggcgctc gtccgcctgg aggaggatgc
     7021 cgcactggcg ccatccggtg cggacggtgg cctcgccctc gccccccccg acacggaccg
     7081 gccgccttgg caccgcttcc cgcgcggcgc cttcgccggc gacttcctcc acgacgtgct
     7141 cgaatggctc gccgcggaag gcttcgcgct cgccaccaac gagggcctgg aggccgcgct
     7201 gcgccggcgc tgcgagcgcg ccggccacgg cgagcgtgcc gacgagcttg tgcagtggat
     7261 gcgcgccctg ctcggcgccc gcctgcccag cctggacgcg gcgctgcagg acctgcgcgt
     7321 gctgatcacc gagatggagt tctggctgcc ctccgctcag ctgcaggcga cggcggtgga
     7381 cgcgctgtgt cggcgccacc tgctccacgc ccagccccgc cccgcactgc cgcagcgcga
     7441 actgcacggc atgctgatgg gcttcgccga cctggtgttc gaacacgacg ggcgctattg
     7501 ggtgctggac tacaagtcga acagcctggg cgaggacggc agcgcctacg accgcgccgc
     7561 actcgaggcg gcgatggcgc atcaccgcta cgacgtgcag gcggcgatct acctgctcgc
     7621 gctgcatcgc ctgctgcgcg cgcgcctggg cggggcctac gagcccgccc gccagctcgg
     7681 cggcgcgctg tatctcttcc tgcgtggcat cgacgggccg gcgcgcggcg aatacgccat
     7741 cccggccacg ccggcgctga tcgagctgct cgacaccctg gaccacctgc tcgccgacga
     7801 ggcttccctg gcatgagccc catccacgct tcgccctcgc ctgccgcggc gccacacgcg
     7861 gcgacagcgc cctccgcgcc gaccgacgcc ctcctcgcca ccctcgacgc ctggacggac
     7921 gccggcctgc tgcgccagct cgacagcgcc ttcgcccgtt tcatcgccag cctcgacccc
     7981 gccgcgccgc ccgcgctgct ggtgggcgca gcggtgctgg cgcgcatgga gggccgcggc
     8041 catagctgcc tgccgctcgc cccgctcgcc gcgaccgacc aggatctgct ggcctggccg
     8101 atggaggccc aagcggcgat cgccgcgctg tggcagaacc tgcccgccga ttccgccgcc
     8161 tggctggcgg cgctgcagca aagccccgtg gtgcgccgca tcggccacga ccccgaactc
     8221 ggccagcccc tgctggtgga cggacccgac accgcaccgc ggctctacct gcgccgttac
     8281 tggcagtacg agcgcagcgt cgccgccgcg gtggcggcgc gctgcgcgca acccgagccg
     8341 gtcgacgagg cgcgtgcgcg cacctggctg gaccgcctgt ttccggccgc gcccggcact
     8401 ccggcccacg aggcggcggt cgactggcag aagctcgcct gcgcgatcgc cttgcgcggg
     8461 cgcctgagca tcctcaccgg cggccccggt accggcaaga cctacaccgc cgcgcgcctg
     8521 ctcgcgctga tgctcgccac ccacccggcg cccgagcgcc tgcgcatcgc gctcgccgcc
     8581 cccaccggca aggccgccgc gcgcctgcgc caggccatcg acggctcgct gcaggccctc
     8641 cagcgcagcc tgggcggcca cctcgacctc gccgcgctga cccgccgcat cggcgccgcg
     8701 cgcaccctgc acgcgctgct cggcgcccgt cccgacacgc ggcgcttccg ccaccacgcc
     8761 ggcaatccgc tcgacgtgga cgtggtgatc gtggacgaag cctcgatggt gcacctggag
     8821 atgatggccg ccctgctcga agccctgccg cgcggcgcgc gcctggtgct gctcggcgac
     8881 aaggaccagc tcgcgtcggt cgaggccggc gcggtgctcg gcgacctctg ccgcgacgcc
     8941 tcggacggac gctacgacgc cgagaccgca cgctacctgc tcgccaccac cggcgaggcc
     9001 ctgcccacgc gctacctgga gcccacggac aaggcgcccg cgctcgccca gcacaccgtg
     9061 atgctgcgcg agagccggcg cttcgagggg ccgatcggcg cgctcgcgca cgcggtcaac
     9121 gcggcgcgcg acccggccct gcccgcgcgc ctgctgcgcg aggatgcgag cggcgcgctg
     9181 cacggggtcg agggcggacc ggtggcggcg gtgatcgcgc tcgcgctcgg cggccgcagg
     9241 ggcgcacccg cctgctaccg cgactatctg gagctgctcg cacgcggacc ggcaggcaag
     9301 gacgccgagg cgcacgccgc gtgggcgacg gcgatcctgc acgccttcga ccgcttccgc
     9361 gtgctgtgcg cggtgcgcga aggcgactgg ggcatggccg ggctcaaccg cgcgatcgag
     9421 cacgcgctcg cgcgcgcgcg tctgctgtcg ccacgcggga cctggtacgc cgggcgcccg
     9481 gtcatggtca cccgcaacga ccccgcgctc ggcgtcttca acggcgacat cggcatcacc
     9541 ctgccggcgc tcgagagcgg cggcctgcgg gtgcacttcc tcgacggcga ggccttgcgc
     9601 tcggtggcgg cgagccgcct cgcccatgtc gagaccgcgt tcgcgatgac ggtgcacaag
     9661 agccagggct cggagttcga gcacacggtg ctggtgctcg ccgcgcagag cggcaacgtg
     9721 ctcgtgcgcg agctgatcta caccgcgatc acgcgcgccc gcaaggcctt cagcctggtg
     9781 gccgaacgcg agggcctgct ggagcatggc gtgttgcagg cgacccggcg cgagagcggg
     9841 ctgctggact ttcagggaac gtagacgatc gccggcgcgg gcgtggaggc ggtgacgggg
     9901 aagacgaagc ggagaacgcg cgaggatgaa cggcggcgtt ctgcacgggg gtcgcatccc
     9961 gaaccgcttc attcgcggct tgcgccgctc ccacgggnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnttc gcggcttgcg ccgctcccac ggggcggggc ggtttccgtg
    10141 ggagcgacgc aagtcgcgat cggggcatcg gcggtttccg tgggagcggc gcaagccgcg
    10201 aaccgggccg tcgggccagc gcctacccct ctacgccacc gccccgccga acaccggcat
    10261 gtgcgcgaag cccgtcttca tcagccggaa cagcgcgtcc tccttcaggg gcggggcgaa
    10321 catgaagccc tggacgaggt cgcagcccat gtccatgagg cgttcgagct gggcctcctg
    10381 ctccacgccc tcggcaaccg tgatgaattc gaaatgccgc gccatgtcga tcacggtgcc
    10441 ggtgaggcgc tggtcggcgg gattggcgtg gagctcgcgg atgaaggcac ggtcgatctt
    10501 gagcaccgag accggcaagg ccttgaggta ggcgagcgag gaatgcccgg tgccgaagtc
    10561 gtcgatcgcg atgcggaagc ccgcgcgcgc gaggtggtcg aggtggcggg cggcgagcgc
    10621 catgtcctgc atcaggccgg attcggtgac ctcgagctcg agcgccgcgg gcggcacgcc
    10681 gctggcctcg acctcggcga cgaggcgctc ggcgaagtcc ggctgcatga gctgctgggt
    10741 cgatacgttg atcgccatgc cgaatacgtc ggcgacgcgc ctcgcctcgc gccagcgccg
    10801 cagcgtggcg agcgcggcgc gcacgaccca gccgccgatc gcgaggatct cgccgctggc
    10861 ctcggcgatg gggacgaact cggcgggcga aacgcgtccg taggcgctgc tgtcccaacg
    10921 cagcagggcc tcgacgccga cgatgtcgcc gctgcacagc tccaccttgg gctggtacac
    10981 cagcgagaac tcgtcgcgcc cgaccgcgcc gcgcaaggcc tcgcggatgc cgttgcggcg
    11041 ctgcatccgc gtctcgagct cggcctcgta ggcctgccac tgcccctcgc ctgcgggcag
    11101 cgcgagcgcc gccatgcgca cgagctgctc cgggctgcgc tcgtccgcct cggcggcgat
    11161 gccgatgcgc agaccgaggt gcagccgcat gtgttcgacc tcgaccggcg ggcggaagcc
    11221 ggcgagcagc cgctcggcga gcgcgccggt cgcgcccctc gccggagcac agtcgcacag
    11281 caggacccac tgcgtgctgt tccacaccgc gaggaagccc tccgggacgc attcgcgcag
    11341 ccgccgcgct gccgcgcgcg cgagctcgtc ggccacgccg aagccgtgca ggcgcgaaac
    11401 ctcgtccatg ccctcgatcg acaggaagag caccgcggcg tcacgccgcg ccgccagcca
    11461 tgcaccgagc tcgtcgagca gcgacagccg gttgggcagg ccggtgagcg gatcgtggcg
    11521 cgcgcgcgcg acgagttcct gcgtgcgttc ggcgaccagc gcttcgagca gttccgcgcg
    11581 ttcggcgcgc tccttcatca ggcgctgcat gtgcaggacg ttgcggatgc gttgcaacac
    11641 ctcgacctgg tcgaagggct tggtgaggaa atcgcgcgcg ccgagttcga gcgcgcggta
    11701 acgggtggca tcgtcgatct gcgcggtgag gatgatgacc gccggcgcct ccgcgccgcc
    11761 cgccgacagc cgctccatga gctcgaggcc gttcaggtgc ggcatgcgca catcgagcag
    11821 gatgaggtcg ggcgcgtgct ccgccacccg cgccgccacc ctgcgcgggt cggtgatgcc
    11881 ttcgaggcgc tggtagcccg cgtcttcgag cagggcgagc agcagctcga cgttggcggg
    11941 gttgtcgtcg acgatcagga tgtcggcgtc ggcctgcatg gggacgcgat cagtcatcgc
    12001 tgctcctgtg ttcgaggatc tgcccgaggc gcgcgacgag ggcaggaacg tcgaagggtt
    12061 tggtcacgta gtcgatgaag cccgcctcgc gcgccttgcg cacggtgtgc ggcgaggccg
    12121 cggcggagat cgccagcacc gggatgtgtg cggtgagcgg gttgcgcgcg agcagggcgc
    12181 gcgcctccag accgctcatg ccgggcaggt cgatgtccat gaggatgagg tcgggcggat
    12241 cggagcaggc cgcctcgatg ccggcctcgg ccgaatccac gctgtgcagc acggcctggc
    12301 cgacgtcctc gaagacgtcc tgcatcagct ggcggttggc gggattgtcc tcgacgtgga
    12361 gcacccggtg gcgccggctc agggcttgcg gcgcggccgc tgcacgcgcc tcgtcgcgga
    12421 ccgccggcgc ctgttcgcac aagggcagct cgaaccagaa cgcgctgccc tcgccctcgc
    12481 ggctgtgcac gccgagctcg ccgtgcatca gatccaccag cttgcgggtg agcgcgagcc
    12541 cgacgccggt gccctcgatg ctgccggact cggcgccgag gcggttgaag ggctggaaga
    12601 gttcgcccag ccggctcgcc gggatgccga tgccggtatc gacgacgccg atccggcagc
    12661 gcccgccgac cgcctcgcag cgcacctcga ccgcgccgcc ggggcggttg tacttgatcg
    12721 cgttgccaag caggttgatg aggatctgct tcacccgggt gtagtcgccc gtgaagcgca
    12781 gcgcgcaggc ctgcgccggc ggcagcgcca gcgcgatgcc ctgctgctcc gcgagcggga
    12841 ggacgatctg ggcggcgtcc tcgatcacgt cgtgcgcgag catgggctcg agcgagacct
    12901 gcagcctgcc cgactcgatg cgggcgagat cgagcacctc gttgatcagc tgcagcaggt
    12961 gcctgccact gcgggcgatc tgctcgacct ggcggagctg gcgctcggac agcggctggc
    13021 ggccgttctg cagcagctgg gcgaagccga ggatggcgtt gagcggcgtg cgcaactcgt
    13081 ggctcatcga ggacaggaac tcggtcttgg cctggttcgc cttctcggcc tggtcgcgcg
    13141 cctgctgcag ctcttcggtg aagcgctgct gctccttgaa caggcggtac aggttgatca
    13201 gcagtgcgca ggtcgaggtg aagggcttga gccagtcgac cagctcgtcc ggatagtcct
    13261 gcggcgcgtt ggcgatcgcg aacatgccga tgacctcgcc gcggtccatg atcggcacgc
    13321 cgaggtagcg cgacagcggc ggatgcccgg gcggcatcgc gccgccgcgc ggatcggacc
    13381 gcatggcgtt ggagagcacc acctcgccgc gggcgaagac gcgcccgagc atgctcgccg
    13441 ggttgctcag gcgcatgtcc ccgctcacca gcttgcgcat cagcccgcgg gtctcgtcgt
    13501 tccaggacag gtcggtgatg gcgtggatct tgagcgccgg gccgccctcg ccgtgctcca
    13561 cctcgccgat caacccatac gcgctgccgg tgagcgcgca cagcgcgtcc ttcaggaagc
    13621 cccacagcgt attgcccgag atcagcgcgt ggtagtcggt gaggccgcgg tgcagcacct
    13681 cgagcagcgc gcgctcctga cgctcgcgct cgcgcacctg atgcacgtcg cgcaggtcgg
    13741 acaggatgcc gatgaagtgg cggccctcga agtcgtccgc ctgcgcgccc tggctcacct
    13801 cgctgacggt gagctgcacc gggatgtggc ggccgtcgcg gtgcagcgcg tcgacctcgg
    13861 tgcccttacc gatcacccgc gccacgccgg tgcgctggta ggcctcgaga tgaccgtcgt
    13921 gcgcggaacg gaagggctcg ggcatcagca tgcgcacgtt gtgcccgcgc agctccgcct
    13981 cttcgtagcc gaacagcgcg caggcgaagc ggttgacctc gatgatccgc ccgcgcccgt
    14041 cgatgcgcag gatgccggcg gccgcgccct cggtgatggc ctggtagcgc gccgcgatgc
    14101 gcgcgctgcg gcgcgcctcg tcggccaggc gctggcgctt gcggtgcagg cggatcaact
    14161 gctcggcctg gcgcgcgagc agccgcaggc gtgcgcgttc ggcctcctcg aagcggcgcg
    14221 gcacggtgtc gatgacgcac agggtgccga gcacgaaccc ctggtccgaa cgcaggggct
    14281 cgccggcgta gaagcgcacc tgcggcgcag cggtgacgag cgggttgtcg gcgaagcgcg
    14341 ggtcgcgtgc ggcgtcctcg accaccaggg gcgcgttgga gaacaccaca tgggtgcaga
    14401 aggagacgtc gcgcggagtc tgcgccacgc acagcccgtg cgccgacttg aaccactggc
    14461 gcgcctcgtc cacgagcgag acgagcgcga tcggcacgcc gaagatcgac gcggccagtt
    14521 gcatcaggtt gtcgaaggcg tcctcgcggg gcgtgtcgag tatcgccagc gcacgcagtt
    14581 cggcgaggcg ctcgggctcg tcggccggca gtcctgggtc gatcatcgcg ggatccatgc
    14641 acgcagggta tgcgtaaagg catcagggta gccgctctcg ccgatctgcg ccgtgagcca
    14701 gcgcacgccc cggcgcatgc ggcgcgagac gccgccgccc ggcgcagccg cgcaaccgcg
    14761 ttcgtcgcgg catggacggg gaggacgaat tgcgcgagac tgcgcggtcc ccgggggacg
    14821 gcctcggccg ccgccgggtt tcttccctgg ccgcacggag atcctgccgg cgatgcgttc
    14881 ggacgagctc gacttccacg cgctgatcga cttcatgccc gacagcggcg cgatccgctt
    14941 ttgcgggcgg cgggcgatcc tcgtccatgc cgactcgatg ggcgcgctgc gcaaggagct
    15001 ggtcgagacc ctgggcagcg agatcgccaa ggtggtgctc acgcgctacg gcttcagctg
    15061 cggctacgag gatgccgagc tgcttgccgc ctacatgcat ccggacagcc tggaggagtt
    15121 catcctcggc ggaccgcgca cccacatgtt ctcgggcatc gccgaggtcg cgcccgatgc
    15181 gctcgagctc gaccatgcca gcgggcgcta ccgcatgagc gggcgctggc ggcactccta
    15241 cgaggccgag cagcacctgc gcctgttcgg tcgcgccgac gagccggtgt gctggacgct
    15301 gaccggctac gcctcgggct tcgcctcgcg ggtgctgcag gccgacatga tctgcgtgga
    15361 gacggcctgc gaggggcgcg gcgatcccca ctgcgcgtgg acgctgatga acgccagcga
    15421 ctgcgcgccc gagctcgcgg gcctgcgcga atacttcaag ccgctcaacc tgcgcgaccg
    15481 cctcaacctg ctcgagagcc gggtgcatga gcgcacccgc gagctcgagg cctccgagca
    15541 acgctaccgc aacctcatcg aggacctccc cgagatggcg ttcgcgctgc acgcctcggg
    15601 gcgcctgctg cacctgaaca aggcggcgcg cctgcgcctc ggggtgagcg aggcgcggct
    15661 gcacgcgctg cgcctgaagg acctcgtgct gcccgactac accgcgcgcg ccaccgcctt
    15721 cctcaaggag atcgcggcac gccgcagcac caccacgctc gacctggtga tgcgcgacgc
    15781 cgcaggcctg cccttcccgg tacggctgca ggtcgagccc gtgctcaagg acgatcgcat
    15841 cgtgggctac agcgggctgg cgctcgacat cagcgcccag ctcgaacgcg agcgcgagct
    15901 caccgagtac gcgcgccgca tcgagtcgcg cgagcagcag atccaggacg tgatcaacga
    15961 ggcggtctat atcctcgacg gccagggccg gctgagcttc gtcaacaccc gcatggccga
    16021 gctgctcggt gccgcacccg aggcggcgat cgggcacgcc gtcggcgact tcatgccggc
    16081 ctcttcggcc gcacgcatcg agcgcgattt tgcccgccgc atcgccggcg aggccggccg
    16141 tcccttcgag atcgcgctct ccggacgcga gggcagcagc gtggtgctgg aggtcaactc
    16201 caccctcttc gtcggccgcg acgtcgccga gggcgtgatc ggcgtggcgc gcgacatcac
    16261 cgcgcgccgg cagatggagc gcgagctcgc gcaggccaac cgcctgagcg cgctcggcca
    16321 gttcgcctcg ggcgtggcgc acgagatcaa caacccgctc ggcctggtgt ccggctatgc
    16381 cgaggagctc caggccctgc tcgacgaatg cgcgccgctc ggcgacgacc ggcggctcgc
    16441 gcagctgcgc cgcggactgg ccaccatcca gcagcaggcc caccgctgca aggcgatcac
    16501 cgccaacctg ctcgccttct cgcgccgcca gagcgccagc ctggagacgg tggacgtggg
    16561 cctcttcgtc gccgaacgcc tggccttctt caacgacgcc ggcctcaccc gcggcgtcga
    16621 gctcgcggtc gacatcgcac cgggtctgcc tcaggccggc accagcccgg ccctgctgga
    16681 acaggtgctg caaaacctga tcaagaacgc ctgcgacgcg atgaacggca gcgggcgcat
    16741 cgacatcgcc gcacgcgccg tcggcgaagg catcgagatc gagatcggcg acagcgggcc
    16801 gggtttcgcg ccgggggtcg ccgaacgcgt gttcgatccc ttcttcacca ccaagccacc
    16861 gggcaagggc accgggctgg gcctgtcgat ctgctacgcg atcatcaacg agctgggtgg
    16921 ccgcatcgcc tgcggcaacc gcccccaggg cggggcctgg ttccgtctgg tgctgccgct
    16981 tgcggaagac ggaccgggag ccgcgaagca tgactgatcg acccatcacc gatcgccacg
    17041 tcctcatcgt cgacgacgag gagctctacc gcgagctgct cggcggccgc ctcggccgcc
    17101 agggctaccg catcagcgag gccggcgacg gcgagggcgc gctgcgctgc atcgagggcg
    17161 gcggtgtcga gctcgccctc atcgacatca agatgccggg catggacggc atcgagctgc
    17221 tcgagcgcat caagcagctc gcgccgcgca ccgaggtcgt ggtgctgacc ggccacggca
    17281 gcatcgacag cgcgatcgcg gcgatgaagc tgggcgcctt cgactacctc accaagccct
    17341 accagctcac cgagctcgac ctcgtggtcg cgcgcgcgct cgaacgcagc gcgctcgcgc
    17401 gccgctgcga cgcgctctcg gtcgaggtgg cgcgcctgcg cagccgcgac gacgacggca
    17461 tggtcgggga cagcccggcg tggctgcgga tgctcgagca ggtgcgccgc gcggcggcgc
    17521 tcgacgtgcc ggtgctgctc accggcgaga gcggcgccgg caaggaggtg gtggccgccg
    17581 cgctgcaccg ctggagccgg cgcgcgggcg aggcctacgt cccggtgaac tgcggcctgc
    17641 tcgaaggcga gctggtcgag agcgagctct tcggccacaa gcgcggcgcc ttcaccggcg
    17701 cgagtgcgga ccgcgagggc ctgttcgagc tggcgagcgc cggcaccctc ttcctcgacg
    17761 agatcggcga gttgccgctc gcctgccagg ccaagctgct gcgcgtactc gacagcggcg
    17821 agttccgccc gctcggcgcc agcgccgtgc gccgcaccgg cgcacgcgtg atcgccgcca
    17881 cccaccgcga cctggaggcg ctcgtggccg cgggccgctt ccgccacgac ctgctgtacc
    17941 ggctcgacgt cgtccacatc gtcgtgccgc cgctgcgcga gcgccgcgag gacatcccgc
    18001 tgctggtcga gcacctgctg caccaggcgg tgcggcgcca ccccggcacg cccgccccgc
    18061 gcgtcgagcc ccaggccctg cagcgcctga tcgactatgc ctggccgggc aacgtgcgcg
    18121 agctgcgcaa cgtggtcgag cgcctgctcg tcttcgccga cggcgaccgc atcgacgcgg
    18181 cgcgcgtcga tgccttgctg caccccggcg ccaggacggt cgcaagcact ggccggaccg
    18241 gcttcgagcg catcgtcccg cttgccgaga tggagcaggc ctacctgcgc tgggtggtgg
    18301 acaagctcga cggcaacgtc agcgccgccg ccacccggct cggcatcgcg cgctcgacca
    18361 tctaccgcct gctgcgcccg cacgcggacg aaggcgcgcg accgtaaggg catgcgggca
    18421 cgccgcaggg tgtgcccgtc gcatcctgca acactgtcgc aggacaggac gctctccccg
    18481 caacccgcgt ccgccgcggg tctcgccctc tcttcgcccc tccgtgcagc gccctgcccg
    18541 gtccccgctg tcgcaaaccg cgacacctcc cctgctgccg gcgaggccgg cacacgcaca
    18601 cgatccgcgc gattgtttga tattcaacaa ttagacgctc cctggcccgc cccttgcttt
    18661 gtactcctcc aacggggaat ccatacccca gcccaggagg agaaccatgc agtcgaacaa
    18721 gatccgaatc ctcgtcgcca gcctcgcgct gagctgtgcc cacatcgccc ttgcggccga
    18781 tccccccgcc gaggggcgcg accgcaccta cgccaaggtg tgcagcttct gccacgacaa
    18841 gggcgtcggc ccggcgatcc tcggtcgcgc gttgcccgcg gcctacatcg agaacgtcgt
    18901 gcgccacggc ttccgcgcga tgccgagctt ccgcagcagc gagatcgaca acgccgccct
    18961 cgccagcgtc gcacgctggg tgtcgcaatc gccggcctcc aaggcctcga cggcctcgcc
    19021 cgcctcgccg gccccgagcg ccgcacccgc cacgaaagcg cagtaggagc cgaccatgga
    19081 cagacgcgaa ttcttccgcc ggacgggcgg cggcatgctg ctcggtgccc cgctcgccag
    19141 cctgcccgcg accgcggcgg cgctggtctc gcctgcgggc gcggcggctg cggtcgtggt
    19201 gatcggccag gccgaccgca gcggcgcggt caccctcgcg caacgcctcg ccgccgcgct
    19261 cggccatggc ggacggccgc tgccggtgca cgcccgcgac gccgccgcgc tgcgccgccc
    19321 cgagcaggtc gacgcgctgc tcgcgctgcc gcacggcacc cgcctcctcg gcgtgatgga
    19381 cgacgcctcg gcgatcgtct tcctggcgat ggcgtcgagc cgcggcgcgc acctgctcgc
    19441 ccacggccac catcgcctcg cctcgcaggg cgcgacccgc catacctgca gcgccaccgg
    19501 ctgcggcgag gcgctcgcct ggcacgaacc ggcctcgcat ccggccacgc acgtcgagcg
    19561 cttctacctc gccaccctcg gcgaaaccac cccgggcgca cgcacgtcgc tgcccgcagt
    19621 cccgggcggc gacgaagcgc tcgcctcctt cctgatccgg ctctgaccct ccggaccacc
    19681 acccgaacgc cattcccctg ccttccgcac ccggagaaac catgaccacc accccgcgca
    19741 acgtgctgcc caagggcgtc agtgcgaacg acttcgaaca ggccctcgcc cagatccgcg
    19801 cgatcgtcgg cgccgaatac gtcgtggccg aggacaccgc cctcgccccc tacaacaaga
    19861 tcatgatggc cgtgcccgac gagcagcacg ccccgtccgc cgcggtgatg cccgacagcg
    19921 tcgagcagat ccaggccatc atgaaggtgc tcaaccagca ccgcatcccg gtgtatccga
    19981 tctccaccgg caagaacctg ggctacggct cggccgcccc ggtcgagcgc ggccagctgg
    20041 tcatggatct gcgccgcatg aaccgcatcc tcgacgtcga tgccgagctg tgcaccgcgc
    20101 tcgtcgagcc cggcgtgacc taccagcagc tctacgacta cctgcaggag aagaagctgc
    20161 cgctgtggtt ctcctgcccc gccccttccg tgatcgccgg cccgctcggc aacatggtcg
    20221 accgcggcgt gggctacacg ccctacggcg agcacttcct gttctcgtcg ggcatggagg
    20281 tggtgctcgc cgacggcgag gtgctgcgca ccggcatggg ctcgatgcca ggctcgaaca
    20341 cctggcaggt cttcaagtgg ggctacggcc cgacgctgga cggcatcttc acccagtcga
    20401 actacggcat cgtcaccaag atgggcatgt ggctgatgcc ggcgccgccc gactaccgtc
    20461 ccttcctgat ccagtacccg gaagacaccg acatcaccaa gatcgtcgac gcgctgcgcc
    20521 cgctgcgcat cgcgatggtg atccccaacg cggtggtgat cgcccacacg ctgtgggagg
    20581 cgccctgcac caaggtcaat cgcgccgact accacccggg cccgggcgcg atcagcgacg
    20641 aggcggtgca gaagatccgc aaggaccaca acctcggcgc ctggaacgtg tatgccgcgc
    20701 tctacggcac gccggagacc aacgcggcga actggaagat catcgagcag gtggccgcgg
    20761 ccaccggcgg cacgctgatc accgactcga ccgaacaggg cgggcgcgcc acccagtacc
    20821 gcttcgacct catgaagggc aagcccaacc tcggcgaatt cgacctctac aactggcgcg
    20881 ggggcggcgg ctcgatgtgg ttcgcgccgg tgtcgcaggc caagggcagc gagacgctca
    20941 agcagatgca gctggccaag ggcatcctcg ccaagtacgg cttcgactac gtgggcgagt
    21001 tcatcgtcgg ttggcgcgac atgcaccacg tcatcgacct gctctacgat cgcagcgatg
    21061 ccgagcagat gaagaaggcc cacgcctgct tcgacgagct gctgcaggcc ttcgcccgcg
    21121 agggctacgg cgtgtatcgc gccaataccg ccttcgccga gaaggtcgcc gccacctacg
    21181 gaccggtcaa gcgcgacgtc gagcgccgcc tcaagcgcgc cctcgacccc aacaacatca
    21241 tcgccccggg acgctgcggc atcagcatct gatcccccac cggcggccgt gcgcacgtgc
    21301 cacgcgtgcg ctgcggccgc ccttgcgcag aggaaaccaa ggatgaacga gatccacctg
    21361 ctgatcggcg gcgaacgtcg ccgcgccacg gacggcgcca gcttcgaacg ccgcaacccg
    21421 ctcgaccacg gcgtcgccac gcgcgctccc gcggccaccg ctgcggacgc ggtggccgcg
    21481 gtcgaggccg ccgccgcggc cttccccgcg tgggccgcca ccggccccgg cgagcgccgc
    21541 gcgctgctga tgaaggcggc gcacgcgctc gaggcgcgcg ccgaggcctt taccgcggcg
    21601 atggccgccg agaccggcgc ctcggcgatc tgggccggct tcaacgtgca cctggcggcc
    21661 ggcatgctgc tcgaggcggc ggcgctgacc acccgcatcg agggcagcat cctgccctcg
    21721 gacgtgcccg gctcggtggc gatggcggtg cgccagcccg ctggcgtggt gctcggcatc
    21781 gcgccctgga acgccccggt gatcctgggc gtgcgcgcga tcgccacccc gctcgcctgc
    21841 ggcaacaccg tggtgctcaa gggctcggag ctgtgcccgg ccacccacgg cctgatcatc
    21901 gaggcgctgc aggacgccgg gctgccggcg ggcgtggtga acttcgtcac caacgccccg
    21961 gccgacgccg gcgcggtggt cgaggccatg gtcgcgcacc cggcgctgcg ccgggtgaac
    22021 ttcaccggct cgacccacgt cggccgcctg atcgcgcaga cctgcgcccg gtacctcaag
    22081 ccggcggtgc tcgagctcgg cggcaaggcg cctttcgtcg tgctcgacga cgccgacctc
    22141 gacgccgcgg tggcggcggc caccttcggc gccttcgcca actcgggcca gatctgcatg
    22201 tccaccgagc gcatcgtcgt cgatgcggcg gtggccgacg acttcgtcgc ccgcctggcg
    22261 gcgcgcgccc gcgccctgcc cctgggcgac ccgcgcaagg gcccggtggt gctcggctcg
    22321 gtggtcgacc agcgcacggt cgagcgctgc aacgcgctga tcgacgatgc gctcgccaag
    22381 ggggcgacgc tggtgtgcgg cggcaaggcc gacagcacgc tgatgcccgc cacgctgctc
    22441 gaccacgtca gcgcgcagat gcgcatctac cacgaggaga ccttcgcccc ggtgaaggcg
    22501 atcgtgcgcg tccagggc
//
LOCUS       DAOQBS010000044        21964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1591_length_21964_cov_14.956091, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000044 DAOQBS010000000
VERSION     DAOQBS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 21964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21964
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1591_length_21964_cov_14.956091"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PKN25_06940"
     CDS             <1..842
                     /locus_tag="PKN25_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV90669.1"
                     /translation="AVTALAADLTRDQHRTKVMAMIGSSIGLVFALSMVAAPLLYAAV
                     GMDGIFWLTAVLAAGAIGVLLWAVPVAPPVPRATGRLIDVLRDGQLMRLNFGVFALHL
                     IQTTMWVMVPSALVSGGGLPVPEHWKVYLPAVLLSFVVMVPAVIVAERHDKLKPVFNA
                     AIVLLAVVQIGLWLFGDGLIPLALLLTLFFISFNVLEATQPSWISRIAPPGSKGTALG
                     VYNTLQSIGLFLGGVLGGWLGQTFGPAAVSLSCAALALLWLALASTMNPPPLRAAPAG
                     APR"
     gene            912..1415
                     /gene="ssb"
                     /locus_tag="PKN25_06945"
     CDS             912..1415
                     /gene="ssb"
                     /locus_tag="PKN25_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNV90670.1"
                     /translation="MASVNKVILIGNLGADPESRFAPSGDAICNIRLATTETWRDKNT
                     GERREATEWHRVAFFGKLAEIAGQYLRKGSQVYIEGSLRTRKWQDQSGQDRYTTEIRA
                     DEMKMLGRREGGDAPMRGGEGGGWDAGAQSAPARQPAAPAQRAQPQSAPPQQSGGFGD
                     FDDDIPF"
     gene            1532..2494
                     /locus_tag="PKN25_06950"
     CDS             1532..2494
                     /locus_tag="PKN25_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV90671.1"
                     /translation="MKDLIHYGDGVHAIDSGYGRPQLAAIHVIVQDGRAAIVDSASNA
                     SVPRILGALAALDIAPEAVDWVLLTHIHLDHAGGAGSLMCALPQARLLVHPRGLRHMI
                     DPGKLWEATCAVYGPERAFDLYGQLVPVPAERIVPATDGLEIHVGSRMLRVFDTPGHA
                     RHHICIWDSVARAFFTGDTFGLSYRELDVDGRAFVVPTTTPTQFDPEGMHASIDRMLA
                     MRPQAMYLTHYSRVGEVDRLGADLHRLIDTMLAVAEAARGDGVARHVEIVAGLEQIFR
                     EEAARQNWALGEEETLELLRMDLELNAQGLGVWLDSRQTGATGA"
     gene            2502..3326
                     /locus_tag="PKN25_06955"
     CDS             2502..3326
                     /locus_tag="PKN25_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNV90672.1"
                     /translation="MTSATTDRARRFGGIDRLYGEGAAARLAGAHACVIGIGGVGSWA
                     AEALARSGVGRLTLIDLDHVAESNINRQAHALEDTLGMAKVSAMATRIAGIDPACRVD
                     TIEDFLTPENAAELLHGFDLVIDAIDNVRAKVAIAVTCRERRIPLVMAGGAGGKRDPA
                     RIGMDDLARTLQDPLLAKVRARLRKEHGFPRDPKKKFGIEAVFSTEPLHFPAVDACDA
                     AHPGAASAPQGLACAGYGSSMAVTASVGLLCAARALEHLLRQADAPARDATPEPSA"
     gene            3323..3709
                     /locus_tag="PKN25_06960"
     CDS             3323..3709
                     /locus_tag="PKN25_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HNV90673.1"
                     /translation="MNAANPSASAIVLFGHGARDPEWAGPMRRIRDAALARPGAPRVE
                     LAFLEFMSPTLPEAIAALAAEGAHKVAVVPIFLAQGGHLKRDLPVLLEEARAAHPGCE
                     ITLATAAGEADPVVAAMADYALGCLG"
     gene            3861..4937
                     /locus_tag="PKN25_06965"
     CDS             3861..4937
                     /locus_tag="PKN25_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV90674.1"
                     /translation="MKPIMLPVPARLLAVLVLSLLTVTSGGVRAELPTVRVEARAVVP
                     AIVAEATIEAVRQSTLAAQMPGRILELSVDAGDRVRRGAVLARIDPAEVAAAVAAADA
                     GIAQAESALANARAEYQRARSLVERKFLSQSALDNARAQFQAAEAQVRAARAQREQAA
                     TVKGYTSVVSPLDGLVAARHIEPGEMAQPGRNLLTVYDPAQMRAVADLAPQRLAELGA
                     GPLRARVELPASRRIVEAAAVTVLPAADARTHTVRVRVDLPAGIEDVLPGSFARVHFQ
                     GTAAAASTAGPTVIPAAAVLRRGELTAVYAADGQGGFTLRQIRLGRALPGGEWVEVLS
                     GLGGDETLALDPVQAGIQARAAAR"
     gene            4952..8242
                     /locus_tag="PKN25_06970"
     CDS             4952..8242
                     /locus_tag="PKN25_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV90675.1"
                     /translation="MEKSLGISGRIAAGFQRNAITPLLALVLLLAGIFATLITPKEEE
                     PQIDVTMANVLVPFPGASARDVEALVARPAEQVLSRMAGVEHVYTVSRPGMAVITVQF
                     EVGVPNQTALVRLYDTVDSHADWLSPQLGVGQPLIKPKGIDDVPIVSFTLWTADPERA
                     AFSLQQVSRAIETELKRIPGTRDVATIGGPGHVIRVDMDIERMNAHGVTAQDLRGALQ
                     LANASQPAGSLVAGNKEVLVQTGTYLESAEDVRNLVVGVSERRPVFLRDVAEVVDGPD
                     QPAQYVWFGTGAAAAERGIDQHGLFPAVTLALSKKPGANAADVAQAAMQRLENLRGSL
                     IPEGVELTVTRDYGKTANDKAKQLIGKLAFATAAVVALVFFALGRREAIIVGIAVSLT
                     LAATLFASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRWHTLEPDTALWKLIP
                     RAVDEVGGPTILATFTVIAALLPMAFVTGLMGPYMSPIPINASMGMFISLVVAFVVTP
                     WLAQKMLKNVDAHQHGGEDRITKRLDGLFRRVMTPMFDPRRGARNRLMLWLAVFALIG
                     ASVALPVVQLVVMKMLPFDNKSEFQVVLDMPAGTPVEDTARVLNEIGAHLATVPEVTD
                     WQSYAGSAAPINFNGLVRQYYLRSAPEQGDIQVNLVDKTARERQSHEIAVSVRDAVTA
                     IALRNGGNAKVVEVPPGPPVLSPIVAEIYGPDYDGQVAVAKRVRAAFEDTPDIVGVDD
                     TVDEDAPKLVLRVDQAKAARMGVAQADIVEVVRLGLSGENVTPVHGGDNKYEIPVRIQ
                     LPAVRQSDLADLLAMQVRARGGDNAGQRVKISELVQVAETRREQILYRKDLLPVVYVT
                     GDMGGKLDSPLYGMFDIRSRVNGMALEGTRGLAGTLGEWFIRQPVDPYAGYQLKWDGE
                     WQITYETFRDMGAAYAVGLVLIYLLVVAQFRSYLVPLIIMAPIPLTIVGVMPGHALLG
                     AQFTATSMIGMIALAGIIVRNSILLVDFVNQQVRGVDGKPGMEFGEALIRAAAVRAKP
                     IGLTALAAMIGAVFILDDPIFNGLAISLIFGIFVSTVLTLVVIPVLYYAAMRGRIASF
                     ENPEENPA"
     gene            8239..8460
                     /locus_tag="PKN25_06975"
     CDS             8239..8460
                     /locus_tag="PKN25_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNV90676.1"
                     /translation="MTTNEYVRAFAGAFVLASLAFGVEASPLFVSKHFLWVTGFVGLN
                     LFQSAFTGICPLVNILHKLGVKDGPASCN"
     gene            complement(8465..8971)
                     /locus_tag="PKN25_06980"
     CDS             complement(8465..8971)
                     /locus_tag="PKN25_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HNV90677.1"
                     /translation="MTLLHRILNLPARVLYLALFLVATGLLGFGLYLQHGLGLEPCPM
                     CIMQRYAFATAALIALIAGLHAPGRGGERIYAVLIGAAALTGGMIAARQSWMQLNPPL
                     IPECGPGLEFMLESFPLAQALPMIFRGAGDCTVIEWTFLGLSIANWSLVSFTATVLFA
                     GWMLLRRS"
     gene            complement(9150..10058)
                     /locus_tag="PKN25_06985"
     CDS             complement(9150..10058)
                     /locus_tag="PKN25_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNV90678.1"
                     /translation="MEQIDPNDLLVFARVVAEGSLTAAADKLGLPKSTVSRRLSRLEE
                     QLGERLLLRTTRRLNITEFGAALLDHARQLESEVEAVAALAESRQARPSGRLRVSMPS
                     DFANLLLTDMLAAFAALHPAVTLELDLSPRRVDLLGENFDLAIRLGDLPDDALLAARR
                     LAVFPWGLYAAPAYLAEHGEPQAPDDLHRHRALRLLARTGDAIRWTLLCGDQRWEGVP
                     PGSITANSPELLIRFACAAAGITTVPEYFAAPHVQRGELRRVLPDWRPPSIAAWAVFP
                     GRRLMPAKTRAFLDMLQAALGGDQRG"
     gene            10154..10753
                     /locus_tag="PKN25_06990"
     CDS             10154..10753
                     /locus_tag="PKN25_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNV90679.1"
                     /translation="MNILQINGSARSQAGNSTRLASEVVARLVAAEPAARVVVRDLAR
                     DPAPAIDEAALGALFTPAEQRSPEQAVRVAQSDALVAEVQAADVLVLGVPMYNFGIST
                     QLKNWIDAIARAGVTFRYTENGPEGLLKGKKAYVTFARGGRYRGTAADTQTPYLKTLL
                     GFLGITDVHFIYAEGLNMGEEATKQGFAEAAADIAAALA"
     gene            10867..11751
                     /locus_tag="PKN25_06995"
     CDS             10867..11751
                     /locus_tag="PKN25_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNV90680.1"
                     /translation="MSTTPKPRIPSATVQRARTVERLVTGHPTSDGAGVKLTRVLTQP
                     LQRRLDPFLMLDAFGSDQADDYIAGFPDHPHRGFETVTYMIAGRMLHRDSAGHEGLLE
                     NGGVQWMTAGRGVIHSEIPQQEEGVMEGFQLWLNLPAQDKMSAPWYRDFAAGELPRFT
                     TSAGVEATVIAGESHGVTGAVTRTVTAPLYLDLHLPAGSSFAQPLAADHNVFVYVYRG
                     QVKLGEVAVPVRNMAILANAADRDGVRIEATEDARVLLIAGRPLGEPIAQYGPFVMNS
                     EQEIYQAVADYRAGRLAA"
     assembly_gap    11887..11986
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12176..13090)
                     /gene="ybiB"
                     /locus_tag="PKN25_07000"
     CDS             complement(12176..13090)
                     /gene="ybiB"
                     /locus_tag="PKN25_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HNV90681.1"
                     /translation="MKYGPIIKEIGRGAKGARDLDTAAAAALFGDILDGVVPELELGA
                     ILLAFRIKSEALPELLGFKQAMDARTVQVAVPEGPRCVVLPTYNGARRQANLMPLVAL
                     LLAREGVPVLIQGRHDFETRVSPFELLAALDLHPAVDAVEASAQLARRHIACIGVDKL
                     LPGLDPLLALRLRMGVRASGHTMAKLIDPCRGRSVRVVAVTHPEYLERMDAFLRADGG
                     RAMLMRGTEGEIYANPRRCPEMKVYVDGEARVAVPAEEGGAPPLAGLPDAPSVADNAA
                     LIRAMLAGEVPVPAPIRAQVAALASVAA"
     gene            13183..14064
                     /locus_tag="PKN25_07005"
     CDS             13183..14064
                     /locus_tag="PKN25_07005"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV90682.1"
                     /translation="MDVGFIGLGLMGRPMALNLLKAGHRVQVWSRRRESMRPLLEAGA
                     GDCASAAEVARRAAVTISMVPDAPDVEQVTLGAEGVADGAAPGHIHIDMSTIAPAVAQ
                     SIAERLGARGVIALDAPVSGGEPGAIAGTLTIMVGGDAAACVHVQPLFEAMGKSITRI
                     GEAGAGQVAKACNQIVTGVGVAAVAEALNFAAKSGADGTKVREALLGGFAYSRILENH
                     GQRMLERNFRPGFKAWMHQKDLRIVMGEAHRLGVALPAAAAAAQLFNGMIGCGMGEDD
                     SVAMLKLLERMSGGVGE"
     assembly_gap    14155..14254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14335..15195
                     /locus_tag="PKN25_07010"
     CDS             14335..15195
                     /locus_tag="PKN25_07010"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV90683.1"
                     /translation="MRVGFIGLGVMGEPMAHHLRAAGHELAVWARRPESAAALVAEGV
                     ALCATPSDLAARSEVVITIVTASADVEALAFGVQGLAEGFAPGAVHVDMSTIAPASAR
                     ALAERYATRGVGWVDAPVSGGGQGARAATLAIMAGAEAATLARVRPLLEALGKRIVHI
                     GPPGAGQVAKACNQMIMVSAIQACAEAFRLAGAHGVDQAAVLCALAGGSAGSRVLEVM
                     GAKMVARDFAAGVEARLHHKDFAILMEEAARLGAPLPVAAQVWQQLDALMGAGWGRDD
                     TASLLRVLEG"
     assembly_gap    15447..15546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15621..16310)
                     /locus_tag="PKN25_07015"
     CDS             complement(15621..16310)
                     /locus_tag="PKN25_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNV90684.1"
                     /translation="MSAGTPGCIHEEAGWRGADDARASLLHTLRAMGAARLNVGTSGN
                     ASQRLEGGRMLISPSGIPAERCRTEDMVVVEADGRYAGARAPSSEWPLHHDVYTAFPA
                     AGAVLHAHAPFATALACQRLDIPPFHYMIARFGGTTVRCARYATFGTQALSDATVAAL
                     QERSACLLANHGMVVLGRDLEHALAMAIEFETLCEQYWRTLQLGEPVLLSEEEMAEVI
                     ERFKWYGKPRA"
     gene            complement(16307..17377)
                     /gene="mtnA"
                     /locus_tag="PKN25_07020"
     CDS             complement(16307..17377)
                     /gene="mtnA"
                     /locus_tag="PKN25_07020"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNV90685.1"
                     /translation="MFEQPIPTLRRDGEVVHVLDQTLLPFRTETVALRTLAEAAHAIR
                     SMQVRGAPLIGATAAFGVALALADEGADDATLAHALATLGATRPTAVNLHWALERMRL
                     ALQPLQPAARVRAAWAEAEAIRADDTATCDAIGRHGVRLIREIAARRPGPVRLMTHCN
                     AGWLATCGAGTALAPVYAAQAEGIAVEVVVSETRPRNQGLLTAWELRAAGVPHILIAD
                     NAAGLLLMRHEVDLVITGADRIAANGDTANKVGTYLKALAARVAEVPFHIAAPHSTLD
                     FACADGAAIPIEDRGADEVLHVRGEAADGATAELALAPAGTRAANPAFDLTPAALITG
                     IVTERGIARPGELARLYPEQAR"
     gene            complement(17411..18568)
                     /locus_tag="PKN25_07025"
     CDS             complement(17411..18568)
                     /locus_tag="PKN25_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNV90686.1"
                     /translation="MPNFPAPIESRLPHVGTTIFTVMSRLAQECGAINLSQGFPDFNA
                     DDLLFERVAHWMRAGHNQYAPMAGTAALRAAIAAKVETLYGTAYDVEREITVTAGATQ
                     ALFTAVTACVRAGDEVIVFEPVYDSYVPAIELAGGKVVRMRLAAPDYRPDWDAVAAAI
                     GPRTRMIMINTPHNPTATVWTRDDLDRLAALVRGTGIVVVADEVYEHIVFDGAAHASC
                     AAHPELAQRSFVVSSFGKTYHITGWKVGYVLAPRELMAEFRKVHQFNVFTVNTPVQLA
                     LADYMADASRHLGLAAFYQAKRDFFRAALAASRFELLPSHGTYFQLARYAAISDLGDA
                     AFCEHLTRNVGVAAIPVSAFFADGRDEHVIRFCFAKNEATLAAACAKLVAL"
     gene            18706..18951
                     /locus_tag="PKN25_07030"
     CDS             18706..18951
                     /locus_tag="PKN25_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HNV90687.1"
                     /translation="MRNLLILILVVFGIWWARGAVRRLKARGGLGRKAEDGRLGKPEV
                     MLECRHCGLIVPESEGVRAGETFYCCDEHRRAGPRPD"
     gene            18929..20524
                     /locus_tag="PKN25_07035"
     CDS             18929..20524
                     /locus_tag="PKN25_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNV90688.1"
                     /translation="MPARDPIEAAAFAVDAADRARRRALRYLNVFRLILGGLFLVAGE
                     ALGLGSESPRLYLVAVVGYLAAVLSLGFPDAVRRLGFERVVTLLALADIVALAAIMWV
                     SGGYRSGMPVLMMVMLAGAGLIGEGRMVMFSAALATLAVLVENASRLFGGGQSVEFLQ
                     VGIFCAGFFGIAAIARLLALRAQANAALAAERGVALAKQQAVSAHILRDMQDGVIVVG
                     ARGRVLHANAQALEMLGLTRAGDGALDGLALADLDAGLEDMLQPAVGEDGRLMRVGAA
                     GRLLRCRRVQGDRGSAAEGDTLLYLTDYEEIQRQLQQIKLAALGRLTASMAHEIRNPL
                     SAVTQAAELLGEEKRADMQARLVRIINDNARRIERMIRDVLALGRREQTMPEALRLAA
                     FVREILDARVFREPAEAQLYELAIDPALTLAMDRAHLHQILDNLLSNAARHASGAPGA
                     VRLWAEALPHAKVVLHVQDDGPGFDEAMRAHLFEPFFTTHPKGTGLGLYIARELAEAN
                     DATLALAPSTPGADFILTASQQP"
     gene            20521..21912
                     /locus_tag="PKN25_07040"
     CDS             20521..21912
                     /locus_tag="PKN25_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV90689.1"
                     /translation="MTQVDRRNRPRAADVLVVDDEDDIRELLELSLMRMGLACDTAGS
                     VGAARALLAERSYRLCLTDMRLPDGDGLELVEYIQTLRPGLPVAVITAFGSIETAIRA
                     LKLGAFDFVTKPVELKALRELVQHALKLHAVEPVEHGAASAERSLIGHSAAFVQLRAQ
                     AGKLARNQAPVFIHGESGTGKEVIARYIHALGARAAGPFVPVNCGAISPELMESEFFG
                     HRKGSFTGATADKSGLFQAAGGGTLFLDEIGELPLAMQVKLLRAIQERAVRPVGAHAE
                     EPVDVRILSASHQDLGRLVAEGRFRQDLFFRINVITLRVPPLRERREDIPDLAAHILA
                     RLAVREGGAPRRLSAAALEALQAYAFPGNVRELENLLERACALCEGEEIGAGDLGVED
                     DCGALAAPGGGENGASPATEAEVAGEGRDEVERLRIIRALEQTRWNRSAAARNLGMTL
                     RQLRYRLQKWGMD"
BASE COUNT         3036 a         7587 c         7851 g         3190 t
ORIGIN      
        1 ccgcggtcac cgcgctcgcc gccgacctca cccgcgacca gcaccgcacc aaggtcatgg
       61 cgatgatcgg ctcctcgatc ggcctggtgt tcgcgctctc gatggtggcg gcaccgctgc
      121 tctacgccgc cgtcggcatg gatggcatct tctggctcac cgccgtgctt gccgcgggtg
      181 cgatcggggt tctgctgtgg gcggtgccgg tcgctccgcc ggtgccgcgc gccaccggcc
      241 ggctcatcga cgtgctgcgc gacggccagc tcatgcggct caacttcggc gtgttcgcgc
      301 tccacctcat ccagaccacg atgtgggtga tggtgccctc ggccctggtc tccggcggcg
      361 gcctgccggt gcccgagcac tggaaggtct acctgccggc ggtgctgctg tccttcgtgg
      421 tcatggtgcc ggcggtgatc gtcgccgagc gccacgacaa gctcaagccg gtgttcaatg
      481 ccgccatcgt cctgctcgcc gtcgtgcaga tcgggctgtg gcttttcggt gacggattga
      541 taccattggc attgttgctg acgctcttct ttatctcctt caacgtgctc gaggccaccc
      601 aaccgtcgtg gatctcgcgt atcgcgccgc ccggctccaa gggcacggcg ctgggcgtct
      661 acaacaccct gcagtcgatc ggcctgttcc tcggcggcgt gctcggcggc tggctggggc
      721 agaccttcgg cccggccgcg gtgagcctgt cgtgcgcggc gctcgcgctg ctctggctcg
      781 cactggcgag cacgatgaat cccccgccgc tgcgcgcggc tccggccggc gcgccgcgct
      841 gagcccggct tcaccatact caccgccggc acgcccgacg cggccggcaa acgcatgcaa
      901 aggagatccg gatggcatcc gtcaataaag tgatcctgat cggcaacctc ggcgccgatc
      961 cggaaagccg cttcgccccc tccggcgacg cgatctgcaa catccgcctg gccaccaccg
     1021 agacctggcg ggacaagaac accggcgagc gccgcgaggc taccgaatgg caccgcgtcg
     1081 ccttcttcgg caagctcgcc gagatcgccg gccagtacct gcgcaagggc agccaggtgt
     1141 acatcgaggg cagcctgcgc acgcgcaagt ggcaggacca gagcggccag gaccgctaca
     1201 ccaccgagat ccgcgccgac gagatgaaga tgctcggccg ccgcgaaggc ggcgatgcgc
     1261 cgatgcgcgg cggcgagggc ggtggctggg atgccggcgc ccaatccgcg ccggcccgcc
     1321 agcccgcagc ccccgcgcag cgcgcgcagc cgcagtcggc gccgccgcag cagtcgggcg
     1381 gcttcggcga cttcgacgac gacattccgt tctgacggat gcctggagcg cgtcctgcgc
     1441 tccgccgact gccgggcggg cgacgcgacg cgtacgctcg cccgtttcac atcgacaggc
     1501 gcggcagcga accggccatt cgggaggaac aatgaaggac ctgatccact acggcgacgg
     1561 cgtgcatgcg atcgactccg gctacggccg gccgcagctc gccgccatcc acgtgatcgt
     1621 gcaggacggg cgcgccgcca tcgtcgatag cgcgagcaac gcctcggtgc cgcgcatcct
     1681 cggtgcgctc gccgcgctcg acatcgcccc cgaggcggtg gactgggtgc tgctgaccca
     1741 catccacctc gaccacgccg gcggtgccgg cagcctgatg tgcgcgctgc cgcaggcgag
     1801 gctgctggtg catccgcgcg gtctgcgcca catgatcgac ccgggcaagc tgtgggaggc
     1861 cacctgcgcg gtgtatggcc cggagcgcgc cttcgacctg tatggccagc tcgtgccggt
     1921 gccggccgag cgcatcgtgc cggccaccga cggcctcgaa atccatgtag gcagccgcat
     1981 gctgcgggtg ttcgacaccc cgggccacgc ccgccaccac atctgcatct gggactcggt
     2041 cgcgcgtgcc ttcttcaccg gcgacacctt cggcctgtct taccgcgaac tcgacgtgga
     2101 tggccgcgcc ttcgtggtgc cgaccaccac gccgacgcag ttcgaccccg agggcatgca
     2161 cgcctcgatc gatcgcatgc tggcgatgcg gccgcaggcg atgtacctga cgcactacag
     2221 ccgcgtcggt gaggtcgatc gcctcggtgc cgacctgcac cgcctgatcg acaccatgct
     2281 cgcggtggcc gaggccgcgc gcggcgacgg cgtcgcccgt cacgtcgaga tcgtcgccgg
     2341 gctggagcag atcttccgcg aggaggccgc gcgccagaac tgggcgctcg gcgaggagga
     2401 gacgctcgag ctgctgcgca tggacctcga gctcaacgcc cagggcctgg gggtgtggct
     2461 ggacagccgc cagactggcg ccaccggtgc ctgagcctgc catgacttcg gccacgaccg
     2521 accgtgcgcg ccggttcggc ggcatcgacc gtctttacgg cgagggcgcg gccgcgcgcc
     2581 tcgcgggcgc gcacgcctgc gtgatcggca tcggcggcgt cggctcgtgg gccgccgagg
     2641 cgctcgcgcg cagcggggtg gggcggctga ccctgatcga cctcgaccac gtcgccgaat
     2701 ccaacatcaa ccgccaggcc catgcgctcg aggacaccct cggcatggcc aaggtgagcg
     2761 ccatggcgac gcgcatcgca ggtatcgacc cggcctgccg ggtggacacg atcgaggatt
     2821 tcctcacccc ggagaacgcc gccgagttgc tgcacggctt cgatctggtg atcgatgcga
     2881 tcgacaacgt gcgcgccaag gtcgcgatcg cggtgacctg cagggagcgc cgcatcccct
     2941 tggtgatggc gggtggggcc ggcggcaagc gcgaccccgc gcgcatcggc atggacgacc
     3001 tcgcgcgtac gctgcaggat ccgcttctcg ccaaggtgcg cgctcgcctg cgcaaggagc
     3061 acggcttccc acgcgacccg aagaagaaat tcggcatcga ggcggtgttc tccaccgaac
     3121 ccttgcactt tcccgcggtc gatgcctgcg acgcggccca tcccggcgcc gcgtccgccc
     3181 ctcaggggct ggcgtgcgcc ggctacggct cgagcatggc ggtgaccgcc agcgtcggcc
     3241 tgctgtgtgc ggcgcgcgca ctcgaacacc tgctgcgtca ggccgatgcg cctgcgcgcg
     3301 acgctacccc ggagccttcc gcatgaacgc agccaatccg tccgcttccg ccatcgtcct
     3361 cttcggccac ggcgcccgcg accccgagtg ggccgggccg atgcggcgca tccgcgatgc
     3421 ggcgctcgcc cgcccggggg cgccgcgcgt cgaactcgcc ttcctcgagt tcatgtcccc
     3481 gaccctgccc gaggcgatcg ccgcgctcgc cgccgagggc gcgcacaaag tggccgtggt
     3541 gccgatcttc cttgcccagg gcggccacct caagcgcgat ctgccggtgt tgctggagga
     3601 ggcgcgcgcc gcccaccccg gctgcgagat caccctcgcg accgccgcgg gcgaggccga
     3661 cccggtggtc gccgcgatgg ccgactacgc gctcggctgc ctgggctgag gcccgacccg
     3721 acggcgcgcg caatccgcgg cgctgccgcg gtgtcggcgg gatggtgact cgcgtcgctc
     3781 ctgcctgcag tagtgccgtc tctcgcggtt ctcgaggggt attccttgac ggccttcaat
     3841 ataagaatat acttattctt atgaagccga tcatgctccc cgttcctgcc cgtctcctgg
     3901 ctgtccttgt cctgagcctg ttgaccgtca cttccggcgg ggtgcgtgcg gagcttccca
     3961 ccgtccgcgt cgaggcacgc gcggtcgtcc ccgcgatcgt cgccgaggcc accatcgagg
     4021 ccgtgcgcca gtccaccctg gccgcgcaga tgcccggtcg catcctcgag ctctccgtgg
     4081 acgccggcga tcgcgtgcgc aggggcgcgg tgctggcgcg catcgacccg gccgaggttg
     4141 ccgccgccgt cgccgcggcc gatgccggca ttgcccaggc cgagagcgcg ctcgccaacg
     4201 cgcgcgccga ataccagcgc gcgcgcagcc tggtcgagcg caagttcctc agccagtcgg
     4261 cgctcgacaa cgcgcgcgcg cagttccagg ccgccgaggc ccaggtgcgc gccgcgcgcg
     4321 cccagcgcga gcaggccgcc accgtgaagg gctacaccag cgtggtgtcg ccgctcgacg
     4381 gcctcgtcgc cgcgcgccac atcgagcccg gcgagatggc acagcccggg cgtaacctgc
     4441 tcaccgtgta tgaccccgcg cagatgcgcg ccgtggccga tctcgccccg cagcgtctcg
     4501 ccgagctggg tgccggcccg ctgcgcgcca gggtcgaact gcccgccagc aggcgcatcg
     4561 tcgaggcggc cgcggtcacg gtgctgcccg ccgccgacgc gcgcacccac accgtgcgcg
     4621 tgcgcgtgga cctgccggcc ggcatcgagg atgtgctccc cggcagcttc gcgcgcgtgc
     4681 atttccaggg cacggccgcc gctgcaagca ccgccgggcc gaccgtgatc ccggctgccg
     4741 ccgtgctgcg tcgcggcgag ctcaccgccg tctacgccgc cgacgggcag ggcggcttca
     4801 cgctgcgcca gatccgtctc ggccgcgcgc tccccggcgg cgagtgggtc gaggtgctct
     4861 ccggtctcgg cggggacgag accctcgccc tcgacccggt gcaggccggc atccaggccc
     4921 gcgcggccgc gcgctgagcc ggaaggcgac catggaaaag tccctcggca tctccggccg
     4981 tatcgcggcc ggtttccagc gcaacgcgat caccccgctg ctggcgctgg tgctgctgct
     5041 ggccggcatc ttcgccacgc tgatcacgcc caaggaagaa gagccgcaga tcgacgtcac
     5101 catggccaac gtgctggtgc ccttcccggg ggcctcggcg cgcgacgtcg aggctttggt
     5161 ggcacggccg gccgagcagg tgttgtcgcg catggcgggc gtcgagcatg tctacaccgt
     5221 gtcgcgcccc ggcatggcgg tgatcaccgt gcagttcgag gtcggcgtgc ccaaccagac
     5281 cgcgctggtg cggctctacg acacggtgga ctcgcatgcc gactggctgt cgccccagct
     5341 cggggtcggc cagccgctga tcaagcccaa gggcatcgac gacgtgccca tcgtcagctt
     5401 cacgctgtgg accgccgatc ccgaacgtgc cgccttctcg ctgcagcagg tctcgcgtgc
     5461 gatcgagacc gagctcaagc gcatccccgg cacccgcgac gtcgccacca tcggcggccc
     5521 gggccacgtg atccgggtgg acatggacat cgagcgcatg aacgcgcacg gcgtcaccgc
     5581 gcaggacctg cgcggcgcgc tgcagctcgc caacgcctcg cagccggcgg gcagcctggt
     5641 cgcgggcaac aaggaagtgc tggtgcagac cggcacctac ctcgagtccg ccgaggacgt
     5701 gcgcaacctg gtcgtgggcg tctccgagcg ccgaccggtc ttcctgcgcg acgtcgccga
     5761 ggtcgtcgac ggccccgacc agcccgcgca gtacgtctgg ttcggcaccg gcgccgccgc
     5821 cgcggagcgc ggcatcgatc agcacggcct cttcccggcg gtcaccctgg cgctgtcgaa
     5881 gaagcccggc gccaacgccg ccgacgtcgc ccaggcggcg atgcagcgcc tggagaacct
     5941 gcgtggcagc ctcatcccgg aaggcgtgga gctcaccgtc acgcgcgatt acggcaagac
     6001 cgccaacgac aaggccaagc agctcatcgg caagctcgcc ttcgccaccg ccgcggtggt
     6061 ggcgctggtg ttcttcgcgc tcggccggcg cgaggcgatc atcgtcggca tcgcggtgtc
     6121 gctcacgctc gcggcgacgc tgttcgcctc ctgggcctgg ggcttcacgc tcaaccgggt
     6181 gtcgctgttc gcgctcatct tctcgatcgg catcctggtc gacgacgcca tcgtcgtggt
     6241 ggagaacatc caccgctggc acaccctcga gcccgacacg gcgctgtgga aactgatccc
     6301 cagggcggtc gacgaggtcg gcggccctac catcctcgcc accttcaccg tcatcgcggc
     6361 gctgctgccg atggccttcg tgaccgggct gatgggtccc tacatgagcc ccatcccgat
     6421 caacgcctcg atgggcatgt tcatctcgct ggtggtggcc ttcgtggtca cgccctggct
     6481 ggcgcagaag atgctgaaga acgtcgacgc ccatcagcat ggtggcgaag accggattac
     6541 gaagcggctc gacggcctct tccgtcgcgt gatgacgccg atgttcgacc cccgccgcgg
     6601 cgctcgcaac cgcctgatgc tgtggctggc cgtgttcgcg ctcatcggcg cctcggtggc
     6661 gctgccggtg gtgcagctcg tggtcatgaa gatgctgccc ttcgacaaca agagcgagtt
     6721 ccaggtcgtg ctcgacatgc cggccggcac gccggtcgag gacacggcgc gcgtgttgaa
     6781 cgagatcggc gcacatctgg ccaccgtgcc cgaggtcacc gactggcagt cctacgccgg
     6841 cagcgcggcg ccgatcaact tcaacggcct tgtgcgccag tactacctga ggagcgcccc
     6901 cgaacagggc gacatccagg tgaacctggt cgacaagacc gcgcgcgagc gccagagcca
     6961 cgagatcgcg gtgtcggtgc gcgacgccgt caccgcgatc gccctccgca acggcggcaa
     7021 cgccaaggtg gtggaggtgc cgcccgggcc gcccgtgctg tcgccgatcg tggccgagat
     7081 ctacggtccg gattacgacg gccaggtggc ggtcgcgaag cgggttcgtg ccgccttcga
     7141 agacaccccc gacatcgtcg gcgtggacga cacggtggac gaggacgcgc ccaagctcgt
     7201 gctgcgcgtc gaccaggcca aggccgcgcg catgggcgtg gcgcaggccg acatcgtcga
     7261 ggtcgtacgc ctcggcctct ccggcgagaa cgtcaccccg gtgcatggcg gcgacaacaa
     7321 gtacgagatc ccggtgcgca tccagctgcc ggcggtgcgc cagtccgacc tcgccgacct
     7381 gctcgccatg caggtgcgcg cgcgcggcgg cgacaacgcc ggccagcggg tgaagatttc
     7441 cgagctggtg caggtggccg agacgcgccg cgagcagatc ctctaccgca aggacctgct
     7501 gccggtggtg tacgtgaccg gcgacatggg tggcaagctg gattcgccgc tgtacggcat
     7561 gttcgacatc cgctcgcgcg tgaacggcat ggcgctcgaa ggtacccggg ggcttgccgg
     7621 cacgctcggc gagtggttca ttcgccagcc cgtcgacccc tacgccggct atcagttgaa
     7681 gtgggacggc gagtggcaga tcacctacga gaccttccgc gacatgggcg cggcctacgc
     7741 cgtgggcctg gtgctgatct acctgctggt ggtggcgcag ttccgcagct acctggtgcc
     7801 gctgatcatc atggcgccga tcccgctcac catcgtcggc gtgatgcccg gtcatgcgct
     7861 gctcggcgcg caattcaccg ccacatcgat gatcggcatg atcgcgctcg ccggcatcat
     7921 cgtgcgcaac tcgatcctgc tggtggattt cgtcaatcag caggtgcgag gggtggatgg
     7981 caagccgggg atggaattcg gcgaggcgct gatccgcgcc gccgcggtgc gcgccaagcc
     8041 gatcggactc accgcgctgg cggcgatgat cggcgcggtg ttcatcctcg acgacccgat
     8101 cttcaacggg ctggcgatca gcctgatctt cggcatcttc gtgtccaccg tgctcaccct
     8161 ggtggtgatc ccggtgctct actacgcggc catgcgtggc cgcatcgcaa gcttcgagaa
     8221 ccccgaggag aatcccgcat gaccaccaat gaatacgtgc gcgccttcgc cggcgccttc
     8281 gtcctggcct cgctggcctt cggcgtcgag gcctcgccgc tcttcgtcag caagcacttc
     8341 ctgtgggtga ccggcttcgt cggcctcaac ctcttccaga gcgccttcac cggcatctgt
     8401 ccgctggtca acatcctgca caaactgggc gtgaaggacg gccccgcgag ctgcaactga
     8461 gtgttcagct acgccgcagc agcatccagc cggcgaacag cacggtcgcg gtgaagctca
     8521 ccagcgacca gttcgcgatc gagagaccga ggaaagtcca ttcgatcacg gtgcagtctc
     8581 ccgcgccgcg gaagatcatc ggcagggcct gcgcgagcgg gaagctctcg agcatgaact
     8641 ccagccccgg gccgcactcc gggatcagcg gcgggttgag ctgcatccag ctctgacgcg
     8701 cagcgatcat gccaccggtg agcgcggccg ctccgatcag caccgcgtag atgcgctcac
     8761 cgccgcgtcc aggtgcatgc aggccggcga tcagcgcgat cagcgcggcg gtcgcgaagg
     8821 cgtagcgctg catgatgcac atcgggcagg gctccaggcc caggccgtgc tgcaggtaga
     8881 ggccgaagcc gagcagcccg gtggcgacca ggaacagggc gaggtagagc acccgcgcag
     8941 gcaggttcag gatgcggtgt agcagggtca tcgtattcga tcttccggga aagggcgcca
     9001 ttgtaggcga gcaggcgagc ggcgtccgcg ctctcggacc cgtgcatgct tgcggtgttc
     9061 cccgtgggcg gcggaggtcg ggggccgccg tgcattccgt gggagcgagg caagcccgct
     9121 tcgcgaggct gtgcaagccc ggggccctct cacccgcgct gatcgccgcc cagcgccgcc
     9181 tgcagcatgt ccaggaaagc ccgcgtcttc gccggcatca gccgccggcc cgggaacacc
     9241 gcccaggcgg cgatcgaggg cgggcgccag tcgggcagca cccggcgcag ctcgccgcgc
     9301 tgcacgtggg gcgcggcgaa atactcgggc acggtggtga tgcccgcagc cgcgcaggcg
     9361 aagcggatca gcagctcggg ggagttcgcc gtgatcgagc ccggcggcac gccttcccag
     9421 cgctgatcgc cgcacagcag ggtccagcgg atcgcgtcgc cggtgcgcgc gagcaggcgc
     9481 agcgcgcgat ggcggtggag atcgtccggc gcctgcggct cgccgtgctc ggcgagatag
     9541 gcgggtgcgg catacagccc ccacggaaac accgccagcc ggcgcgcggc gagcagcgcg
     9601 tcgtccggca ggtcgccgag gcggatcgcg aggtcgaagt tctcgccgag caggtccacc
     9661 cggcgtggcg acaggtcgag ctccagcgtc accgcgggat gcagcgcggc gaaggcggcg
     9721 agcatgtcgg tgagcagcag gttggcgaag tcgctcggca tcgacacccg cagccgcccg
     9781 ctcgggcgcg cctggcggct ctcggcgagc gccgcgaccg cctcgacctc gctctcgagc
     9841 tggcgtgcgt ggtcgagcag ggcggcgccg aactcggtga tgttgagacg acgggtggtg
     9901 cgcagcagca ggcgctcgcc gagctgctcc tccagccgcg acagccgacg cgagacggtg
     9961 gacttgggca ggccgagctt gtcggccgcg gcggtcaggc tgccctcggc caccacgcgg
    10021 gcgaagacga gcaggtcgtt ggggtcgatt tgttccatat agggaacaat catgtgcgtc
    10081 gaacgggctt aatgcaatcc ccggcatcca atacactgca atccatccca tccccaagcc
    10141 agaggacaag cacatgaaca tcctgcagat caacggtagc gcccgttccc aagccggtaa
    10201 ctccacccgc ctcgccagcg aagttgtcgc ccgcctggtc gccgctgagc cggccgcccg
    10261 tgtggtggtg cgcgacctcg cccgcgaccc tgcgccggcg atcgacgagg ccgcgctcgg
    10321 cgccctgttc accccggccg agcagcgcag ccccgagcag gcggtgcgcg tggcgcagag
    10381 cgacgcgctg gtcgccgagg tacaggcggc cgacgtgctt gtgctgggcg tgccgatgta
    10441 caacttcggc atctccaccc agctcaagaa ctggatcgac gcgatcgctc gcgccggcgt
    10501 caccttccgc tacaccgaga acggtcccga gggcctgctc aagggcaaga aggcttatgt
    10561 cacctttgcg cgcggtgggc gctaccgcgg caccgcggcc gacacccaga cgccttacct
    10621 gaagaccctg ctcggttttc tcggcatcac cgacgtgcat ttcatctacg ccgaaggcct
    10681 gaacatgggt gaagaggcca cgaagcaggg cttcgccgag gccgcggcgg acatcgccgc
    10741 cgcgctcgcc tgagcccttg cggcacgagc ctcgcgaccg ccgccggcgt cgcgaggccc
    10801 gcctgaacga cgagggcggg cccacccgcc ccacaccccc ccgaccccgg acagaggagc
    10861 acgcagatga gcacgacccc caagccccgc atcccctccg cgaccgtgca gcgtgcgcgc
    10921 acggtggagc ggctcgtgac cggccatccg acttcggacg gtgccggtgt caagctcacc
    10981 cgcgtgctca cccagccgct gcagcgccgg ctcgatccct tcctgatgct cgacgccttc
    11041 ggcagcgacc aggccgacga ctacatcgcc ggcttccccg accatcccca ccgcggcttc
    11101 gagaccgtga cctacatgat cgccggccgc atgctgcatc gcgacagcgc cggccacgag
    11161 ggcctgctgg agaacggcgg cgtgcagtgg atgaccgccg gccgcggcgt gatccactcc
    11221 gagatcccgc agcaggaaga gggcgtgatg gaaggcttcc agctctggtt gaacctgcca
    11281 gcacaggaca agatgagcgc gccctggtat cgcgacttcg ccgcggggga gctgccgcgc
    11341 ttcaccacct ccgccggcgt cgaggccacc gtgatcgccg gcgagagcca tggcgtgacc
    11401 ggcgccgtca cccgcacggt caccgcgccg ctctacctcg acctgcacct gcccgccggt
    11461 tcgagcttcg cgcaacccct ggcggccgac cacaacgtct tcgtttacgt gtaccgcggg
    11521 caggtgaagc ttggcgaggt ggcggtgccg gtgcgcaaca tggcgatcct cgccaacgcc
    11581 gcggatcgcg acggcgtgcg cattgaggcg accgaggacg cccgcgtgct gctgatcgcc
    11641 ggccgcccgc tcggcgagcc gatcgcgcaa tacggcccgt tcgtgatgaa cagcgagcag
    11701 gagatctacc aagccgtagc cgactatcgc gccggccgcc tggccgcctg aagagagacc
    11761 tgcggttcgt ccgcgctggc ggcgatcgcg ggagaggcgc cgcggtcgcc ccgaaaaccg
    11821 ccgcgcttcc tttgccccgc tgcgatcgcg acttgcgtcg ctcctacggg aaccgccgcg
    11881 cttccgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttgc cccgctgcga
    12001 tcgcgacttg cgtcgctcct acgggaaccg ccgcgcttcc gaagcagtgc tgtaggagcg
    12061 acgcaagtcg cgattacggt gcttcaggcc gcgccgtcgc gtgctgctcc ggctcccggc
    12121 cgtatcgcga cttgcggagc ggctcgggaa cgatgggaag tcgggtcgga tgcgctcagg
    12181 cggccacgga tgcgagggcg gcgacctggg cgcggatcgg cgccggcacg ggaacctcgc
    12241 ccgcgagcat ggcgcggatc aacgcagcgt tgtccgccac cgagggcgca tccggcagcc
    12301 cggcgagcgg gggggcgccg ccttcctctg cggggacagc gactctggcc tcgccatcca
    12361 cgtagacctt catttccgga caacgtcgcg ggttggcgta gatctcgccc tcggtgccgc
    12421 gcatcagcat cgcgcggccg ccgtcggcgc gcaggaaggc gtccatgcgc tcgaggtact
    12481 cggggtgggt caccgccacc acgcgtacgc tgcgtccgcg gcaggggtcg atcagcttgg
    12541 ccatggtgtg gccgcttgcg cgcacgccca tgcgcaggcg cagcgcgagc agcggatcca
    12601 gcccgggcag cagcttgtcc acaccgatgc aggcgatgtg ccgccgcgcg agttgcgcgg
    12661 aagcctcgac ggcgtcgacg gccggatgca ggtcgagcgc ggcgagcagc tcgaaggggc
    12721 tcacccgcgt ctcgaagtcg tggcggccct ggatcagaac cggcacgccc tcgcgcgcga
    12781 gcaacagcgc caccagcggc atcaggttgg cctggcggcg cgcgccgttg taggtcggca
    12841 gtaccacgca gcgcggaccc tcgggcaccg ccacctgcac ggtgcgtgcg tccatcgcct
    12901 gcttgaagcc gagcagctcg ggcagcgcct cgctcttgat gcggaaggcg agcaggatgg
    12961 cgccgagctc gagctcgggc accacgccgt cgaggatgtc gccgaagagc gccgccgccg
    13021 cggccgtgtc gaggtcgcgg gcgcccttgg cgccccgccc gatttccttg atgattggac
    13081 cgtatttcat ggcggcgatt atggcgagct tcgcgccgcg cgaccagcgt gtccacgcgc
    13141 cgcacccgcc gcagaacaag caatcccgtg gagactcgac gaatggatgt cggattcatc
    13201 gggcttggcc tcatgggccg gcccatggcg ctcaatttgc tgaaagcggg ccatcgcgtg
    13261 caggtgtgga gccggcggcg cgagtcgatg cggccgctgc tggaggccgg cgcgggcgac
    13321 tgcgcgagtg cggccgaggt ggcgcggcgc gcggcggtga cgatcagcat ggtgccggac
    13381 gcgcccgacg tcgagcaggt gacgctgggt gccgagggcg tggccgacgg tgccgcaccc
    13441 ggccacatcc acatcgacat gagcacgatc gcgcccgctg tggcgcagtc catcgccgag
    13501 cgcctggggg cgcgcggggt gatcgcgctc gatgcgccgg tttcgggcgg cgagccgggc
    13561 gcgattgcgg gcacgctcac catcatggtg ggcggcgacg cggcggcctg cgtgcacgtg
    13621 cagccgctct tcgaagccat gggcaagtcg atcacccgca tcggcgaggc cggtgctggc
    13681 caggtcgcca aggcctgcaa tcagatcgtc accggggtgg gcgtggcggc ggtggccgag
    13741 gcgctcaatt tcgcggccaa gagcggcgcg gacggcacga aggtgcgcga ggcgctgctc
    13801 ggcggcttcg cgtattcgcg catcctggag aaccatgggc agcgcatgct cgagcgcaac
    13861 ttccggcccg gcttcaaggc ctggatgcac cagaaggatc tgcgcatcgt catgggcgag
    13921 gcgcaccggc tcggcgtggc gctgcccgca gcggcggccg cggcgcagct cttcaacggc
    13981 atgatcggct gcgggatggg tgaggacgat tcggtggcga tgctcaagct gctggagcgc
    14041 atgagcggcg gggtaggcga atgagcgcgc ccgcacagcc ggccgaaggg aagcgctgcc
    14101 ctctcccggg gaggaggtcg cgcagtgaca ggagggtggt ccggtgagcg tggcnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctctc ccggggagga ggtcgcgcag
    14281 tgacaggagg gtggtccggt gagcgtggcg gaaggcaggg cggagggcgg ggcgatgagg
    14341 gtcggtttca tcgggctggg cgtgatgggc gagccgatgg cgcaccacct gcgcgccgcg
    14401 ggccacgagc tcgcggtgtg ggcgcggcgg ccggagtcgg cggcggcgct ggtcgccgag
    14461 ggcgtggcgc tgtgcgcgac gccttctgat ctggctgcgc gcagcgaggt ggtgatcacc
    14521 atcgtcaccg cgagcgctga cgtcgaagcg ctcgccttcg gtgtgcaagg gctggccgag
    14581 ggcttcgcgc ccggggccgt ccatgtggac atgagcacga tcgccccggc gtcggcgcgc
    14641 gcgctcgcgg agcgctacgc cacgcgcggc gtcggctggg tggacgcgcc ggtgtcgggc
    14701 ggcggacagg gcgcgcgcgc ggccacgctg gcgatcatgg ccggtgccga ggccgccacg
    14761 ctggcgcgcg tgcgtccgct gctcgaggcg ctcggcaagc gcatcgtgca catcggcccg
    14821 ccgggcgccg ggcaggtggc caaggcctgc aaccagatga tcatggtgtc ggcgatccag
    14881 gcctgcgccg aagccttccg cctcgccggc gcccatgggg tggaccaggc cgcggtgctg
    14941 tgcgcgctcg ctggcgggtc ggcgggctcg cgggtgctgg aggtgatggg cgcgaagatg
    15001 gtggcgcgcg acttcgccgc cggcgtcgag gcccgcctgc accacaagga tttcgccatc
    15061 ctgatggaag aggccgcccg cctcggcgcg ccgctgccgg tggcggcgca ggtttggcag
    15121 cagctcgacg cgctgatggg cgcaggctgg ggtcgggacg acaccgcgag cctgctgcgg
    15181 gtgctggagg ggtagcggag ggcctgttcg cctctggcgc gcgggtctcg tcgcccgcct
    15241 gcgccaacgg acatcgcttt cgtcggcggc tgaatcgcga cttgcgtcgc tcctacgaaa
    15301 accgtcgtgc tcatcgttgc cgctggcggc tgggcggtct tgtgggagcg acgcaagtcg
    15361 cgatcgcggc gcttacgcgc tcggtggcgc gcatcgcttt cgtcggcggc tgaatcgcga
    15421 cttgcgtcgc tcctacgaaa accgtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnngtgc tcatcgttgc cgctggcggc tgggcggtct tgtgggagcg atgcgagtcg
    15601 cgatgcgtct tgtcgtcggg tcacgcgcgt ggcttgccgt accacttgaa gcgctcgatc
    15661 acctcggcca tctcctcttc cgacagcagc accggctcgc ccagctgcag ggtgcgccag
    15721 tactgctcgc agagggtctc gaactcgatc gccatcgcca gcgcgtgctc gaggtcgcgg
    15781 ccgaggacga ccatgccgtg gttggcgagc aggcaggcgc tgcgctcttg cagcgccgcc
    15841 acggtggcgt cggacagcgc ctgggtgccg aaagtggcgt agcgcgcaca gcgcaccgtg
    15901 gtgccgccga agcgcgcgat catgtagtgg aagggaggga tgtcgaggcg ctggcaggcg
    15961 agtgcggtcg cgaagggcgc atgcgcgtgc agcaccgcgc cggccgccgg gaaggcggta
    16021 tagacgtcgt ggtgcagcgg ccattcggac gagggcgcgc gcgcgccggc gtagcggccg
    16081 tcggcctcca cgaccaccat gtcctcggtg cggcagcgct cggcggggat cccgctcggg
    16141 ctgatcagca tgcgcccgcc ctcgaggcgc tggctggcgt tgcccgaggt gccgacgttg
    16201 agccgcgccg cgcccatcgc gcgcagggtg tggagcaggc tggcgcgcgc gtcgtccgcg
    16261 ccgcgccagc cggcttcctc gtggatgcag ccgggcgtgc cggcgctcac cgtgcctgct
    16321 ccgggtagag gcgggcgagc tcgcccgggc gggcgatgcc gcgctcggtg acgatgccgg
    16381 tgatgagcgc agccggggtg aggtcgaagg cggggttggc ggcgcgcgtg cccgcggggg
    16441 cgagcgcgag ctcggcggtg gcgccgtcgg cggcctcgcc gcgcacgtgc aggacctcgt
    16501 cggcgccgcg gtcctcgatc gggatggcgg cgccgtcggc gcaggcgaag tcgagcgtgg
    16561 agtgcggtgc ggcgatgtgg aagggcacct cggcgacgcg ggcggcgagc gccttgaggt
    16621 aggtgccgac cttgttggcg gtgtcgccgt tggcggcgat gcggtcggcg ccggtgatga
    16681 cgaggtcgac ctcgtggcgc atcagcagca ggccggcggc gttgtcggcg atgaggatgt
    16741 gcggcacgcc ggcagcgcgc agctcccagg cggtgagcag accctggttg cgcggccgcg
    16801 tctcgctcac cacgacctcg accgcgatgc cctcggcctg cgccgcatac accggcgcga
    16861 gcgcggtgcc ggcgccgcag gtggcgagcc agccggcgtt gcagtgcgtc atcagccgca
    16921 ccgggccggg acggcgggcg gcgatctcgc ggatcaggcg cacgccgtgg cggccgatgg
    16981 cgtcgcaggt cgcggtgtcg tcggcgcgga tcgcctcggc ctcggcccag gccgcccgca
    17041 cgcgtgcagc cggctgcagc ggctgcagcg cgaggcgcat gcgctcgagc gcccagtgca
    17101 ggttcaccgc ggtgggccgg gtggcaccca gggtggcgag cgcgtgcgcg agcgtggcgt
    17161 cgtcggcgcc ctcgtcggcg agcgcgagcg ccaccccgaa ggccgcggtg gcgccgatca
    17221 gcggcgcgcc gcgcacctgc atgctgcgga tcgcgtgcgc ggcctcggca agggtgcgca
    17281 gcgcgacggt ctcggtgcgg aagggcagca aggtctggtc gaggacatgg acgacctcgc
    17341 cgtcgcggcg caaggtgggg atgggttgtt cgaacatcgg ggcactctcg tggagggggg
    17401 gcggggcgac ttagagcgcc accagtttgg cgcaggcggc ggcgagcgtg gcctcgttct
    17461 tggcgaagca gaatcggatc acgtgttcgt cgcggccgtc ggcgaagaag gccgacaccg
    17521 ggatcgcggc gacgccgaca ttgcgggtca ggtgctcgca gaaggcggcg tcgcccaggt
    17581 cggagatcgc cgcatagcgc gcgagctgga agtaggtgcc gtgcgagggc agcagctcga
    17641 agcgcgaggc ggccagcgcg gcgcggaaga agtcgcgctt ggcctggtag aacgcggcca
    17701 ggccgaggtg gcgcgaggcg tcggccatgt agtcggcgag cgcgagctgt accggagtgt
    17761 tcacggtgaa cacgttgaac tgatgcacct tgcggaattc ggccatgagc tcgcgcgggg
    17821 cgagcacgta gcccaccttc cagccggtga tgtggtaggt cttgccgaag ctcgacacca
    17881 cgaagctgcg ttgcgcgagc tcggggtgcg cggcgcagga ggcgtgcgcc gcgccgtcga
    17941 agacgatgtg ctcgtagacc tcgtcggcca ccacgacgat gccggtgccg cgcaccagcg
    18001 ccgcgaggcg gtcgagatcg tcccgcgtcc ataccgtggc ggtggggttg tgcggggtgt
    18061 tgatcatgat catgcgcgtg cgcggcccga tcgccgccgc caccgcgtcc cagtcggggc
    18121 ggtagtcggg tgcggccaag cgcatgcgca cgaccttgcc gccggcgagc tcgatggcgg
    18181 ggacgtagga gtcgtacacc ggctcgaaca cgatgacctc gtcgcccgcg cgcacgcagg
    18241 cagtcaccgc ggtgaacagc gcctgggtgg cgccggcggt gacggtgatc tcgcgctcga
    18301 cgtcgtaggc cgtgccgtag agcgtctcga ccttggcggc gatcgccgcg cgcagcgccg
    18361 cggtgccggc catgggcgcg tactggttgt ggccggcacg catccagtgc gcgacgcgct
    18421 cgaacagcag gtcgtcggcg ttgaagtcgg ggaagccctg cgacaggttg atcgcgccgc
    18481 attcctgggc gaggcgggac atcacggtga agatcgtcgt gccgacgtgc ggcaggcggg
    18541 actcgatcgg cgcggggaag ttcggcatgg cggcggctcg gtgcggtgcg gaccggcatt
    18601 gtcgcaaacc ggccgggtgc gcggtaggca cggcgtgcgc ggccgagggc gccggggggc
    18661 gcatgttaga atccgggtgt tctcgacttc catccggccc ccaacgtgcg caacctgctc
    18721 atcctgatcc ttgtcgtgtt cggcatctgg tgggcgcgcg gcgcggtgcg ccgcctcaag
    18781 gcgcgtggcg gactcgggcg caaggccgag gacgggcgcc tgggcaagcc cgaggtcatg
    18841 ctcgagtgcc gacactgcgg gctgatcgtg cccgagtccg agggcgtgcg cgccggcgaa
    18901 accttctatt gctgcgacga acatcgccgt gccggcccgc gacccgattg aagcggccgc
    18961 cttcgcggtc gatgcggccg accgcgcgcg ccggcgtgcg ctgcgttacc tcaacgtctt
    19021 ccggctgatc ctgggcggtc tcttcctcgt cgccggcgag gcgctcggcc tcggcagcga
    19081 gagcccgcgc ctctacctcg tggcggtggt cggctacctc gccgcggtgc tcagtctcgg
    19141 tttccccgac gcggtgcgac ggctcggctt cgaacgcgtc gtgaccctgc tcgcgctcgc
    19201 cgacatcgtc gcgctcgcgg cgatcatgtg ggtgagcggc ggctaccgta gcggcatgcc
    19261 ggtgctgatg atggtgatgc tcgccggtgc aggcctgatc ggcgaagggc gcatggtgat
    19321 gttctcggcc gcgctcgcca ccctggcagt gctggtcgag aacgcctcgc ggcttttcgg
    19381 tggggggcag tcggtcgagt tcctgcaggt cggcatcttt tgcgccggct ttttcggcat
    19441 cgcggcgatc gcgcgcctgc tcgcgctgcg cgcccaggcc aacgccgcgc tagcggccga
    19501 gcgcggcgtg gcgctcgcca agcagcaggc ggtgagcgcc cacatcctgc gcgacatgca
    19561 ggacggcgtg atcgtggtcg gcgcgcgcgg ccgcgtcctc cacgccaacg cgcaggcgct
    19621 cgagatgctc ggcctgaccc gtgccggcga cggggcgctc gacggactcg cgctcgccga
    19681 tctcgacgcc gggctggagg acatgctgca gccggcggtg ggcgaggacg ggcgtctgat
    19741 gcgggtgggc gcggccggcc gcctgctgcg ctgccgccgc gtgcagggcg accgggggag
    19801 cgcggccgag ggcgacaccc tgctgtatct aaccgactac gaggagatcc agcgccagct
    19861 gcagcagatc aagctcgccg cgctcggccg cctcaccgca agcatggcgc acgagatccg
    19921 caacccgctg tcggcggtga cccaggccgc cgagctgctc ggcgaggaaa aacgcgccga
    19981 catgcaggcg cgcctggtgc gcatcatcaa cgataatgcg cgccgcatcg agcgcatgat
    20041 ccgcgacgtg ctcgccctcg gccggcgcga gcagaccatg cccgaggccc tgcgcctggc
    20101 cgccttcgtg cgcgagatcc tcgatgcgcg cgtgttccgc gagcccgccg aggcgcagct
    20161 gtacgagctt gcgatcgacc ctgcgctgac cctggcgatg gaccgcgccc acctgcacca
    20221 gatcctcgac aacctgctgt cgaacgcggc ccgccatgcc tcgggcgcgc ccggcgcggt
    20281 gcgcctgtgg gcggaggccc tgccgcacgc caaggtcgtg ctgcacgtgc aggacgacgg
    20341 tccgggcttc gacgaggcga tgcgggcgca cctgttcgaa cctttcttca ccacccatcc
    20401 caagggtacc ggcctcggac tgtacatcgc gcgcgagctg gccgaggcca acgacgccac
    20461 cctggcgctc gccccgagta cccccggtgc cgacttcatc ctcaccgcga gccagcagcc
    20521 atgacccagg tcgaccgccg caaccggccc cgtgccgccg acgtcctcgt cgtcgatgac
    20581 gaggacgaca tccgggaact gctcgagctc tcgctgatgc gcatgggact ggcctgcgac
    20641 acggcgggct cggtgggcgc ggcgcgtgcg ctgctggccg agcgcagcta ccggctgtgc
    20701 ctgaccgaca tgcgcctgcc cgacggcgac gggctggagc tggtcgagta catccagacg
    20761 ctgcggccgg gcctgccggt ggcggtgatc accgccttcg gcagcatcga gaccgcgatc
    20821 cgcgcgctca agctgggtgc cttcgacttc gtcaccaagc cggtcgagct caaggccttg
    20881 cgcgaactcg tgcagcacgc gctcaagctg cacgcggtgg agccggtcga gcatggtgcg
    20941 gcgtcggcgg agcgcagcct gatcggccac tcggcggcct tcgtgcagct gcgcgcccag
    21001 gccgggaagc ttgcgcgcaa ccaggcgccg gtgttcatcc atggtgagtc cgggaccggc
    21061 aaggaggtga ttgcgcgcta catccacgcg ctcggtgcgc gcgccgccgg ccccttcgtg
    21121 ccggtcaact gcggggcgat ctcgcccgag ctgatggaga gcgagttctt cggtcatcgc
    21181 aagggcagct tcactggcgc caccgcggac aagagcgggc tgtttcaggc cgcgggcggc
    21241 ggcaccctgt tcctcgacga gatcggcgaa ctgccgttgg cgatgcaggt caagctgctg
    21301 cgcgcgatcc aggagcgcgc ggtgcgcccg gtgggtgcgc atgccgagga gccggtggat
    21361 gtgcgcatcc tctccgcgtc gcaccaggat ctcggccgcc tggtggccga ggggcgcttc
    21421 cgccaggacc tgttcttccg catcaacgtg atcaccttgc gcgtgccgcc gttgcgcgag
    21481 cgccgcgagg acatccccga cctggccgcc cacatcctcg cccgcctcgc cgtgcgcgag
    21541 ggtggggcgc cgcgccggct ttccgcggcg gcgctcgagg cgctgcaggc ctacgctttc
    21601 cccggcaacg tgcgcgagct ggagaacctg ctcgagcgcg cctgtgcgct gtgcgagggt
    21661 gaggagatcg gtgccggcga cctcggggtg gaggacgatt gcggcgcgct cgcggcgccg
    21721 ggggggggcg agaatggtgc atcgccggcg accgaggccg aagtggccgg cgagggccgg
    21781 gatgaggtcg agcgcctgcg catcatccgc gcgctcgagc agacgcgctg gaaccggtcg
    21841 gcggccgcgc gcaacctcgg catgaccttg cgtcagctgc gctaccgttt gcagaagtgg
    21901 ggaatggact agcgggcgca ggccgcggac gctggtgcgg cggggccgcc gcgggtggac
    21961 gtcg
//
LOCUS       DAOQBS010000045        21519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1654_length_21519_cov_17.856923, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000045 DAOQBS010000000
VERSION     DAOQBS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 21519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21519
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1654_length_21519_cov_17.856923"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PKN25_07045"
     CDS             <1..1098
                     /locus_tag="PKN25_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNV90690.1"
                     /translation="WGRYAGRDVLPLWVADMDFAAPPAVIEALHRRIDHGVFGYTDAW
                     PALEQAVVEGLRRDHGWTIQPDWLVWLPGVVAGFNLACAIAGEAGDGVLTATPVYPPF
                     LTAPANTGRVLQRAELVLGDGRWQWDWDALEAACTPTTRLLLLCSPHNPVGRVFDEAE
                     LRRLADFAARHDLLVCSDEIHCGLVLDADRTHRPLAALDEAIARRTITLMAPSKTWNI
                     PALYCAFAIIPDPALRRRYRHAMRGIVPHVNVLGMVAAEAAYRDGDAWRRALLDYLRG
                     NRERVLEALGAMPGLTTTRPEATYLAWVDCRAMMAARGVSDPAAFFEAAGVGLSDGAA
                     FGAPGFVRLNFGCPRATLDEALARMARALGA"
     gene            complement(1275..>3654)
                     /locus_tag="PKN25_07050"
     CDS             complement(1275..>3654)
                     /locus_tag="PKN25_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV90691.1"
                     /translation="LAPDIALAVDAQTALDGARAADHATPPPAAPASLPAAVPAAEAG
                     AVDSAQDDIDSQLLPIFLEEAAETIGELHATLRRWRMQADDEEAPRAVARLLHSLKGS
                     ARMAGAMRLGAHLHELESRLEDGLAAREAAAGLAEALAHGIDRCEQVIDELALGEAAL
                     QAPAAPIRAEEAGAGAEAAPEVEPLASPTLRVRAERIDRFVNQAGEIGVARSRVEGEL
                     RSLRRSLLDLTENVIRLRNQLREVEIQAEVQMQSRIAQAGTQHTDFDPLELDRYTRLQ
                     ELTRMMAESVNDVTTVQQGLLHNLDGAELALGSQARMARELQQGLMQVRMLPFDNLAS
                     RLYRVVRQSARELGKRAALDLRGGRIEVDRSVLEQMAAPLEHLLRNAVAHGIEAPAAR
                     LAAGKAETGNIELTVRQEGNEIAIEIADDGAGLDLERIAAQARARGLLGADERVDERR
                     LVNLIFVSGFSTAREVSAVSGRGVGMDVVKAQTAAAGGRVEVASRPGAGTRFSIRLPL
                     TLAVTQTLLVEALGRSWAVPANMIVQAMELKPDALQAMQRAHGIEWQGEHYPYRYLPR
                     LLGDREARPAEARYSWVLLLRAGAQTLALHVDGLRGNQEVIVKNAGPQLTRVVGMSGA
                     TVLGDGEIVLILNPVALGARAATTTDEAHAGLFGPAAEAPPPLHLPTVMVVDDSLTVR
                     KITGRLLEREGYRVLTAKDGVDALEQLIDLKPDVVLTDIEMPRMDGFDLVRNIRADAR
                     LHDLPVVVITSRLAEKHRRYALEVGADHYLGKPYQEEELLALVARYAQAGKG"
     assembly_gap    3655..3754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3755..7068)
                     /locus_tag="PKN25_07055"
     CDS             complement(<3755..7068)
                     /locus_tag="PKN25_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNV90692.1"
                     /translation="MTHATEPDLGPLTWVKGEIDAALGRAREILERGCAGSEAAGLQF
                     AQSHLHQVRGALDIVGLDGLTLFTDALEQLLGAMARGEVPADAARHALALRALAMVGN
                     YLEELADGAPDQPLRLAARYLELAAARGQHDASAADLFHPDLSRRPPARAAPPADTTG
                     SEARELRAARSRFERGLLEWLRKGAAGEGARVMRDAIAEVEAQQRSPAARSLWWASLA
                     FYDALIHGGLQPDPAVKRLCTQLDAQFRRLLSGTPSVPDRLLRELLYQIARAPVRTEQ
                     QRAVRACWQLDAQIPEPGLEVGETPIAPLLDALAARLGEARAHWEAFCAGTAVALPRF
                     EDAVAAMSAPAEALGRPGVRILCAALLDFARWLRRDPLQFVETSGMEVATALLSLEGA
                     LDRRPPPAGFGARAEQIAARLLALQRGEPLSAGAIVAAPADARQAQERAALEQVAKEM
                     LASLAHVEQVLDDFFRNQARREPLLQLHQPLQQIIGALSMLGETDALALVRNAAIHIA
                     SLAREDSRAGQAEFERLARDLSALGFYIEAMPRGGARLEELLDPSLGLQGRPAAQEDA
                     AAPAAAIAATPVAAPAPTPATEAAAAAPVEMPEQPPPAAAEPPPQDLDAAATQVAAAH
                     PESPENLFGLDFPDLDFSPLEELETPAAMPQAATEPVGTDARAPAAAPELAATVAAES
                     AAAPMAAGAAPVGAEIDAELLAIFIEEAHEVLAGIAAGLEALRDGTDEPAQLVTIRRG
                     FHTLKGSGRMVGLVQLGDTAWAVEQALNRWLQLDWALTPALFALVAEGHRVFSAWVAQ
                     LEHGEAGGGDTAALIAEARRLHEAAEPVHDAGITTPSGALPPLPEPIEEIEAEPAATA
                     PASAPMQIDSVDFLLDGEIGGAPPAPPAGTEETTPVFPAATVASATEEDLIEFIEPLD
                     EGAPIALEAFEFFPAQPDEVRIEGGHDEVAPAQAPAATQDDAPASDVAASPAGDTLRI
                     GDGEISRALHALYCSESAQHLAALEQALRALHAGDEPPSTAAIRAAHTLAGISGTVRA
                     EAAHRLGRALEHRLERLADATRAPDGNELALFGEALDALRAMRDEIAAGGTPAAEDAL
                     VARLDARADEDSAGLA"
     gene            complement(7098..9212)
                     /locus_tag="PKN25_07060"
     CDS             complement(7098..9212)
                     /locus_tag="PKN25_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV90693.1"
                     /translation="MALNPFGKKAPAPGAGTEAQTIIETSAPEQTVANKTPSPPARSG
                     GRLGMLGGLFVACTAISAGLLAYQARDASLSALQLGAASELQVLPGQMARAARLARLG
                     EPGAVADLEQTRARFAGLLQTLGQGGSHAGATLPPLPASMRPLLQDLDAEWQKNATLA
                     ARLAAQGAPLAGTSAAVRAINAASGALADAADQFGRRVAAMNGDASALRAAHALLLHA
                     QRATLQANTLFSADADPAAAAKVLAGEAEQLRQRLAELERPSNGSVLDESLRALGSAA
                     APILQAIERIGADAGALQQANAASAGLAANTPALLAASTALGTALAGRTGGFGPLHAG
                     AALAALLAVFVLAQMARAYAADAAARRLASEAERRQAEDERNLTQQAILRLMNEMGDL
                     ADGDLTVRATVTEDITGAIADSVNYTIEELSVLVRRINDAATRVTSATESARRTSTEL
                     LAATDRQSQEIEEAGATAERMAQSMTDSSARALQSAQVARRSLESARKGADAVQNSIR
                     GMNDIRSQIQETAKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAATAGEAG
                     RGFTVVAEEVQRLAERSAEATKQIAAIVKTIQTDTQDAVGAMESATRNVVEGAQLSDA
                     AGQALAEIDQVSTETASLIERISADVQEQAATAGRVAAAMKDILAVTEQTSLGTRQTA
                     VSIDQLAELAVELKGSVSGFKV"
     gene            complement(9262..9768)
                     /locus_tag="PKN25_07065"
     CDS             complement(9262..9768)
                     /locus_tag="PKN25_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV90694.1"
                     /translation="MARSSLREFQERLARRLAEAAGAERRGLLGFQCGSDNWLIELAE
                     AGEILPPPPLSAVPLTRPWFRGLANVRGSLYGVVDFAAFNGQPPISAGGRARLLLIGA
                     RHGIHSALLVSSSSGLRSHEDFEPAPGGDAPPWVARRLRDMQGRAWLQLDPARLLAQP
                     AFLDAGLE"
     gene            complement(9774..10139)
                     /locus_tag="PKN25_07070"
     CDS             complement(9774..10139)
                     /locus_tag="PKN25_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV90695.1"
                     /translation="MPIRKILVVDDSPVERLALQEVLGRNGYQVLTAENGEQAIARSR
                     SDHPDLILMDVVMPGMNGYQATRTISRDDETRDIPIIMCTSKGQETDKIWGMRQGALD
                     YLVKPVDHDELLARIRAMG"
     gene            complement(10159..10560)
                     /locus_tag="PKN25_07075"
     CDS             complement(10159..10560)
                     /locus_tag="PKN25_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV90696.1"
                     /translation="MDLAGLKVMVIDDSNTIRRSAEIFLAQAGCEVVLAEDGFDALAK
                     IADHHPDVIFVDIMMPRLDGYQTCALIKKNPRLSPTPVIMLSSRDGLFDRARGRMAGS
                     DEYLTKPFTKDSLLRAVASHARRGGDAARAG"
     gene            complement(10601..10753)
                     /locus_tag="PKN25_07080"
     CDS             complement(10601..10753)
                     /locus_tag="PKN25_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNV90697.1"
                     /translation="MCLICGFIYDEASGLPDEGIAPGTRWEDVPPNWTCPECNARKED
                     FEMVEI"
     gene            10872..11756
                     /locus_tag="PKN25_07085"
     CDS             10872..11756
                     /locus_tag="PKN25_07085"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNV90698.1"
                     /translation="MPLAIPETPPCVLCLSAGDATAGGGLAADILTLASMGCHPLPVQ
                     TAALVRDTRGIDELWPVAPELIAMQARAVLEDVPVAAFKLGFCGSVENVAVIAEILAD
                     YPDVPLVVEPGLHAAALLGEAGEEIAAALADLILPQTTLLVADRHELLRLTGLAGDEA
                     AESDEDDGGGGGQGETGGAELDEALERLLGNGTEYVLLTGGGDHGPQLVDTLFGSEGI
                     VRTDAGPRPAAPCLGAGTTLAAAAVAAMAHGMALPEAVREAQEFTRQALRHAYRAGMG
                     RALPDRFFWARGKGESDD"
     gene            11749..12381
                     /gene="thiE"
                     /locus_tag="PKN25_07090"
     CDS             11749..12381
                     /gene="thiE"
                     /locus_tag="PKN25_07090"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNV90699.1"
                     /translation="MTERFPARTLRGLYAVTPDEPDTGRLLALAQSVLAGHPALLQYR
                     NKGADAGLRREQALALRDACRAAGVPFIVNDDLALALAIDADGAHLGREDGPPAAARA
                     ALGPQRILGLTCYADWSRATEGAEIGADYIAFGAMFPSTTKPHAPPAPFALVERARRE
                     LGLPVAAIGGITLERAPQVIAAGADLLAVVGDVFGAADPAARARMYRALF"
     gene            12799..13713
                     /locus_tag="PKN25_07095"
     CDS             12799..13713
                     /locus_tag="PKN25_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HNV90700.1"
                     /translation="MDEEKWKNLEALITGLDKVVKAAAVNTRTRSLADRVTALQSDFA
                     SARASGDATMRDHIEMAVQELWANVERWSGVAPKRKISEWLADFSIAGLIGLVVLLVV
                     LGLLVAGIMLFTSAVGWKALMGVDGTRPILTVAAIIATLAYGGGLIFSALYSNEGKFE
                     ERFRMAREIFLVFSGVFATIVGFHFGAGNNGTGGAGGVENVQQLELDVRRGAKPGELE
                     LSVTGGTPPYTVTTTGTNIKTISPAQYDRSPIVIGITREDPEKSWTVSFSVVDRVGTK
                     KQIEPKREVLDALFGGQSGAGSGAGGGG"
     gene            13955..15238
                     /gene="hemL"
                     /locus_tag="PKN25_07100"
     CDS             13955..15238
                     /gene="hemL"
                     /locus_tag="PKN25_07100"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNV90701.1"
                     /translation="MTSRNEALFQRAQRSIPGGVNSPVRAFRSVGGTPRFLARAEGAR
                     VWDADGKEYIDYVGSWGPAIAGHAHPAIVEAVREAALKGLSFGAPTESEVDMAELICA
                     MLPSVEMVRLVSSGTEATMSAIRLARGFTGRDAIVKFEGCYHGHADSLLVKAGSGLLT
                     FGNPSSGGVPADFAKHTIVLDYNDLQQVEDVFKARGDEIAAIIVEPVAGNMNLIKPQP
                     GFLEGLRRICTEYGAVLIFDEVMTGFRVGPQGVQGLYGITPDLTTLGKVIGGGMPVGA
                     FGGRRDIMEKIAPLGSVYQAGTLSGSPVAVAAGMVSLQLTREAGFYERLTASTQRLVA
                     GLAAAAKDAGVTFSADSVGGMFGVYFADKVPASFAQVMATDKERFNRFFHAMLEAGHY
                     FAPSAFEAGFVSVAHGEAEIDATVAVARAVFAQLG"
     gene            complement(15305..16747)
                     /gene="mgtE"
                     /locus_tag="PKN25_07105"
     CDS             complement(15305..16747)
                     /gene="mgtE"
                     /locus_tag="PKN25_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNV90702.1"
                     /translation="MTEPEELHADDVQQHLKEVQDLLARQKVVEGLVHRQDMPRHELV
                     ENLVHKQHEAGLRAKLDSLHPADIAYILEALPREERLYVWDLVKAERDGEILLEVSDA
                     VRESLIETMDPEELKAAAGSLDADELADLAPDLPPEVIEDVFQSLDSEGREQLRAAMS
                     YPEDSVGALMDFDMVTVRDDVTLEVVLRYLRRFDELPDHTDKLFVVDREDHLEGILTL
                     ETLLISDPEKTVAEVMRSEPVISFTPEDEADDAAQAFERYDLVSAPVIDSERRVIGRI
                     TVADAVDFIREESEAEILSQAGLREEEDIFASVWSSVKNRWAWLAINLVTAFVASRVI
                     GLFEESIEKLVALAALMPIVAGIGGNSGNQTVTMIVRAIAMGQVEPSAMTRLLRKELG
                     VALINGVVWGGLLGVLAWWLYGNVDLGLVMTGAMTLNLLLAACAGVLVPMLRQRLGAD
                     PALGGSVMITALTDSGGFFIFLGLATLFLL"
     gene            complement(16882..17589)
                     /gene="mtgA"
                     /locus_tag="PKN25_07110"
     CDS             complement(16882..17589)
                     /gene="mtgA"
                     /locus_tag="PKN25_07110"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNV90703.1"
                     /translation="MPIPAPRRILGWVARGLGVALLLALFWQAVLLTQVVWWSRFDPG
                     STSFMRIRLAELREADPRAELRQQWVPYERISIHLKRAVVAAEDDRFLDHDGFDWEGI
                     ERALERNAEEGRVTAGGSTISQQLAKNLFLSSSRSWLRKGQEAAITLMIEATWDKRRI
                     LEVYLNVAEWGNGVFGAEAAARHYYGRPAASLGPAEAARLAVMLPNPRRYEKTFGPRL
                     AAHAARVQRRMANSQVP"
     gene            complement(17597..18430)
                     /gene="aroE"
                     /locus_tag="PKN25_07115"
     CDS             complement(17597..18430)
                     /gene="aroE"
                     /locus_tag="PKN25_07115"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNV90704.1"
                     /translation="MDRYAVVGNPIAHSKSPWIHAAFARQTGQALTYEALLAPLDDFA
                     ATVARFRAEGGRGLNVTVPFKLEAFALADRRTPRAEAAGAVNTLAFGTDGILGDNTDG
                     AGLVRDLASHLGCPPRGRRILLLGAGGATRGALLPLLDTAPADLCIANRTAHKAVELA
                     QAFAAHAGTVRIEGCGFEALADQRFDLVINATSASLSGELPPLPPGLYAQGALAYDMM
                     YGRGHTPFLRAALADGAARVADGLGMLVEQAAESFALWRGVRPDSAPVLAELRQLIEA
                     G"
     gene            complement(18441..19364)
                     /locus_tag="PKN25_07120"
     CDS             complement(18441..19364)
                     /locus_tag="PKN25_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNV90705.1"
                     /translation="MTPPAAPVLPAGALPGAWSGRLRRFGGPLLVAAFVISISLHALL
                     LLIHFRLPEAKPARDKGLEVVLVNARHARAPDKAEVLAQANVDAGGNTEQEVRASTPL
                     PPQPNRQDGDALVEAKRRTPEPVREQKQVITRDKAPAKVAAAPKKAEQPTPTPEPQRE
                     ISGLDLLDSAAAVARIEAQIDRDLKELAKRPRKKFIGARAQEYRFAQYVEDWRQKVER
                     IGTLNYPDAARGRLYGSLLLSVTIRSDGTVERMGVHRSSGHKVLDDAALRIVKMAAPY
                     APFPPDIRKDYDVIEITRTWTFTNSDQVRAN"
     gene            complement(19372..21279)
                     /locus_tag="PKN25_07125"
     CDS             complement(19372..21279)
                     /locus_tag="PKN25_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNV90706.1"
                     /translation="MFVLYEEDGAFKAGTILADNDATLQVENTHGKRVKLKRAHVLME
                     FREPGPSDLLARAEAEAEGLDVEFLWEVCGDDEFAFADFAAEYHGHVPNAVESAALLL
                     RLHSAPIWFHRKGRGRFRKAPADILQAALAGLEKKRQQAAAIEHMRAELVAGRLPPEL
                     AALLPQALYRPDRNRPEIKALEAACVDSGLSAARLLLACGALASSYDFHYNRFLFEHF
                     PEGTAFPAIEPPVLPTALPRAEVAAFSIDDASTTEIDDAFSVTPRAEGGWRIGIHIAA
                     PALGFARGSTLDAVARRRLSTVYMPGNKITMLPDEIVQTFTLGEGRDCPAVSLYLDIT
                     PGLAIVHQESRVELVPIVANLRHHDIEPVFNDDTVHNGGPDFQWKRELTLLWDLATVL
                     EAGRGKAAGNEDRIDFGFSVDWTEETADGPGRVSIGRRLRGSPMDKLVAELMIHANMT
                     WGKLLDTSGIPGLYRAQGGGKVRMTTVAAPHEGLGVDCYAWSSSPLRRYVDLVNQWQI
                     ISVLQGTPPAFAPKSAELMAAMRDFELTYAEYAEFQRGMERYWCLRWLRQHGRGEVEA
                     TVLRENVVRLAEIPLVIRVPSMPLQMPGSRVKLVVERTDLLDVEVEARFVQTLAEPDP
                     ETVDEAGFGFA"
BASE COUNT         2805 a         7324 c         8028 g         3262 t
ORIGIN      
        1 tggggccgct acgccggccg cgacgtgctg ccgctgtggg tcgccgacat ggacttcgcc
       61 gcgccgccgg cggtcatcga ggccctgcac cggcgcatcg accatggtgt gttcggctac
      121 accgatgcct ggcccgcgct cgagcaggcc gtggtcgaag gcctgcggcg ggatcacgga
      181 tggacgatcc agcccgactg gctggtgtgg ctgccgggcg tggtcgccgg cttcaacctc
      241 gcctgcgcca tcgccggcga agcgggcgac ggcgtgctca ccgccacccc ggtgtatccg
      301 cctttcctta ccgccccggc caacaccggc cgcgtgctgc agcgcgccga actggtgctg
      361 ggcgacggcc gctggcaatg ggactgggac gcgctcgagg ccgcctgcac gcccacgacc
      421 cggctgctgc tgctgtgcag cccgcacaac ccggtcggcc gggtgttcga cgaagccgaa
      481 ctgcgccggc tcgccgactt cgccgcgcgc cacgacctgc tggtgtgctc ggacgagatc
      541 cactgcggcc tggtgctcga cgccgatcgc acgcaccgcc cgctcgccgc gctcgacgag
      601 gctatcgcgc ggcgcacgat caccctgatg gcgccgtcca agacctggaa catcccggcg
      661 ctgtactgcg ccttcgcgat cattcccgac cccgcgctgc gccggcgcta ccgccacgcc
      721 atgcgcggca tcgtgccgca tgtgaacgtg ctcggcatgg tcgcggcgga ggccgcctac
      781 cgcgacggcg atgcctggcg gcgggcgctg ctggattacc tgcgcggcaa ccgcgagcgc
      841 gtgctggagg cgctgggtgc gatgcccggc ctgacgacga cgcgccccga agcgacctac
      901 ctggcctggg tcgactgccg cgcgatgatg gcggcgcgcg gggtctccga cccggcggcc
      961 ttcttcgagg ccgccggcgt cggactctcg gatggcgcgg ccttcggcgc gccgggcttc
     1021 gtgcggctca acttcggctg cccgcgcgcg acgctggacg aagcgctcgc gcgcatggcg
     1081 cgcgcgctgg gcgcgtagac gccgggacgg agcggtcttc cactgcaata tctcgacttg
     1141 cgtcgctcct gcagcgtgct tccacgccgg gggcgcagga gcgacgcacg tcgagatcgg
     1201 cggcggttcg gagggcgtcg aggcgcatcc gttgccgcat cccggctggc accgcagaaa
     1261 gagcgaggcc cccctcagcc cttgcccgcc tgtgcgtagc gcgccaccag cgcgagcagc
     1321 tcctcttcct gatagggctt gccgaggtag tggtcggcgc cgacctccag ggcgtaacgg
     1381 cggtgcttct ccgccaggcg cgaggtgatc accaccaccg gcaggtcgtg caggcgcgcg
     1441 tcggcgcgga tgttgcggac gaggtcgaag ccgtccatgc gcggcatctc gatgtcggtg
     1501 agcaccacgt cgggcttcag gtcgatgagc tgctcgagcg cgtccacgcc gtccttggcg
     1561 gtgagcacgc gatagccctc gcgttcgagc aggcgaccgg tgatcttgcg cacggtcagc
     1621 gagtcgtcca ccaccatcac cgtgggcagg tgcagcggcg gcggcgcctc ggcggcggga
     1681 ccgaacaacc ccgcgtgcgc ctcgtccgtg gtggtggcgg cgcgcgcgcc gagcgcgacc
     1741 gggttgagga tcagcacgat ctcgccgtcg ccaagcacgg tcgcgccgct catgccgacg
     1801 acgcgggtga gctgcgggcc ggcgttcttg acgatgactt cctggttgcc gcgcagcccg
     1861 tcgacatgca gcgcgagcgt ctgcgcgccg gcgcgcagca gcagcaccca gctgtagcgc
     1921 gcctcggcgg ggcgcgcctc gcgatcgccg agcaggcgtg gcagatagcg gtagggataa
     1981 tgctcgccct gccattcgat gccgtgcgcc cgctgcatgg cctgcagcgc gtcgggcttg
     2041 agctccatgg cctgcacgat catgttggcg ggaacggccc agctgcggcc gagcgcctcc
     2101 accagcaagg tctgggttac cgcgagggtg agcggcaggc ggatcgagaa gcgggtgccg
     2161 gcgccgggcc ggctggcgac ctcgacgcgg ccaccggcgg cggcggtttg cgccttcacc
     2221 acgtccatgc cgacgccgcg gccggagacc gcggagacct cgcgcgcggt gctgaagccg
     2281 gagacgaaga tcaggttcac caggcggcgc tcgtcgacgc gctcgtcggc gccgagcaga
     2341 ccgcgcgcgc gcgcctgcgc cgcgatgcgc tcgaggtcga gcccggcgcc gtcgtcggcg
     2401 atctcgatgg cgatctcgtt gccctcctgg cgcaccgtca gctcgatgtt gccggtctcg
     2461 gccttgccgg cggccaggcg cgcggccggg gcctcgatgc catgcgcgac cgcgttgcgc
     2521 agcaggtgtt cgagcggcgc ggccatctgc tcgagcacac tgcggtcgac ctcgatgcgt
     2581 ccgccgcgca ggtcgagcgc ggcacgcttg ccgagctcgc gcgcgctctg ccgcaccacc
     2641 cggtagagcc ggctggcaag gttgtcgaag ggcagcatgc gcacctgcat cagcccttgc
     2701 tgcagctcgc gcgccatgcg cgcctggctg ccgagggcga gctccgcgcc gtccaggttg
     2761 tgcagcaggc cctgctgcac ggtggtcacg tcgttgacgc tctcggccat catccgcgtg
     2821 agctcctgca ggcgggtgta gcggtcgagc tcgagcggat cgaaatcggt gtgctgggtg
     2881 ccggcctgcg cgatgcgcga ttgcatctgc acctcggcct ggatctcgac ctcgcgcagc
     2941 tggttgcgca ggcggatcac gttctcggtg agatcgagca gcgagcgccg caggctgcgc
     3001 agctcgccct cgacccggct gcgcgcgacg ccgatctcgc cggcctggtt gacgaagcgg
     3061 tcgatccgct cggcgcgcac acgcagggtc ggggaggcga gcggctcgac ctcgggcgcc
     3121 gcctccgcgc cggcgccggc ttcctcggcg cggatcgggg cggccggcgc ctggagggcc
     3181 gcttcgccga gggcgagctc gtcgatgacc tgctcgcagc ggtcgatgcc gtgcgcgagc
     3241 gcttcggcga gcccggccgc cgcctcgcgc gcggcgaggc cgtcctccag gcgcgactcg
     3301 agttcgtgca ggtgggcgcc gaggcgcatc gcgcccgcca tgcgcgcgct gcccttgagc
     3361 gagtgcagca ggcgcgcgac cgcacgcggc gcctcttcgt cgtcggcctg catgcgccag
     3421 cggcgcaggg tggcgtggag ctcgccgatg gtctcggcgg cctcttcgag gaagatcggc
     3481 agcagctggc tgtcgatgtc gtcctgcgca ctgtccacgg cgcccgcctc cgccgcgggg
     3541 accgctgcgg gtaggctcgc gggagccgct ggcgggggtg tcgcgtggtc ggcggcgcgc
     3601 gcgccgtcga gtgcggtctg cgcgtcgaca gcgagggcga tgtcgggagc gaggnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcggcc aggcctgcgc tgtcttcgtc
     3781 ggcgcgagcg tccaggcgtg cgacgagtgc atcctccgcc gccggcgtgc cgcccgcggc
     3841 gatctcgtcg cgcatcgcgc gcagggcgtc gagggcctcg ccgaacagcg ccagctcgtt
     3901 cccgtccgga gcccgggtgg cgtcggcgag gcgttccagc cggtgctcca gggcgcggcc
     3961 gaggcgatgc gcggcctctg cgcgcacggt gcccgagatg ccggccaggg tatgggcggc
     4021 gcgtatcgcg gcggtcgagg ggggctcgtc gcccgcgtgc agcgcgcgca gcgcctgctc
     4081 gagcgcggcc aggtgctggg ccgattcgct gcagtagagc gcgtgcagcg cacgcgagat
     4141 ctcgccgtcg ccgatgcgca gggtgtcgcc agcgggactg gcggcgacat cgctggcggg
     4201 cgcgtcgtcc tgcgttgcgg cgggcgcctg tgcgggggcg acctcgtcgt gtccgccttc
     4261 gattcgcact tcgtccggct gcgccgggaa gaactcgaag gcttccagcg cgatcggggc
     4321 gccctcgtcg agcggctcga tgaattcgat caggtcttct tcggttgcgc tcgccacggt
     4381 cgcggcgggg aacacgggcg tggtctcctc cgtacccgct gggggggccg gcggggctcc
     4441 gccgatctcg ccgtcgagca ggaagtccac gctgtcgatc tgcatcggcg cggaggcggg
     4501 ggccgtggcg gcgggttccg cctcgatctc ctcgatcggc tcggggaggg gcgggagcgc
     4561 tccgctcggc gtggtgatgc cggcgtcgtg caccggctcg gccgcttcgt ggagccgtct
     4621 cgcctcggcg atcagagccg cggtgtcgcc accgccggcc tcgccatgtt cgagttgcgc
     4681 cacccaggcg gagaacaccc gatggccctc ggcgacgagg gcgaacaagg cgggggtgag
     4741 cgcccagtcg agttgcagcc agcggttcag cgcctgctcc accgcccacg cggtgtcgcc
     4801 gagctggacg agcccgacca tgcgcccgct acccttgagg gtgtggaagc cgcgccggat
     4861 ggtgaccagt tgcgcgggct cgtccgtgcc gtcgcggagt gcttcgagcc cggccgcgat
     4921 cccggcgagc acctcgtgcg cctcctcgat gaagatcgcc agcagttcgg cgtcgatctc
     4981 cgcgcccacc ggcgccgccc ctgcggccat cggggcggct gcgctctccg cggcgaccgt
     5041 ggcggcgagt tcgggggcgg cggcaggcgc ccgcgcatcg gtgccgacgg gctctgtcgc
     5101 ggcctgcggc attgcggcag gtgtctcgag ttcttcgagg gggctgaagt cgagatcggg
     5161 gaagtcgagg ccgaacaggt tttcggggct ctccgggtgg gccgctgcga cctgcgtcgc
     5221 cgcggcgtcc aggtcttgtg gaggcggctc cgcggccgcg ggcggcggct gctcgggcat
     5281 ctcgacgggc gccgctgcgg cggcttcggt cgcgggcgtc ggtgccggtg ccgccacagg
     5341 tgtggcggcg atcgcggcag cgggcgccgc cgcgtcctcc tgtgcagccg gcctgccctg
     5401 gagccccagg ctcgggtcga gcagttcttc caggcgggcg ccgccgcgcg gcatcgcctc
     5461 gatgtagaag ccgagcgcgg agaggtcgcg cgcgaggcgt tcgaactcgg cctggccggc
     5521 gcggctgtcc tcgcgcgcga ggctggcgat gtggatcgcg gcgttgcgca ccagggcgag
     5581 cgcgtcggtc tcgccgagca tgctcagggc accgatgatc tgctgcagcg gctggtggag
     5641 ctgcagcagg ggctcgcgcc tggcctggtt gcggaagaag tcgtccagca cctgttcgac
     5701 gtgggcgagg ctggcgagca tctccttcgc cacctgctcg agcgcggcgc gctcctgcgc
     5761 ctggcgggca tccgccggtg ctgcgacgat cgcgcccgcg gacaggggct cgccgcgctg
     5821 cagggcgagc agacgggcgg cgatctgttc cgcgcgcgcg ccgaatccgg cgggcggcgg
     5881 acgacggtcg agcgcgcctt cgaggctcag cagcgcggtg gcgacctcca tgcccgaggt
     5941 ctcgacgaac tgcagcggat cgcggcgcag ccagcgcgcg aagtcgagca gtgcggcgca
     6001 caggatgcgc acgcccgggc ggccgagcgc ctcggcgggg gcgctcatcg ccgcgactgc
     6061 atcctcgaag cggggcaggg ccaccgcggt gccggcgcag aaggcttccc aatgcgcgcg
     6121 cgcctcgccg aggcgggcgg cgagggcgtc gagcagcggc gcgatcggtg tctcgccgac
     6181 ctccagcccc ggctccggga tctgcgcgtc gagctgccag cacgcgcgta ccgcacgctg
     6241 ctgttcagtg cgcaccgggg cgcgcgcgat ctggtagagg agttcgcgca gcagacggtc
     6301 cggcaccgag ggcgtgccgc tcagcaggcg gcggaactgg gcgtcgagct gggtgcacag
     6361 gcgcttcacc gccgggtcgg gctgcagccc gccgtggatc agggcgtcgt agaacgcgag
     6421 gctggcccac cacagactgc gcgcagccgg gctgcgctgc tgcgcctcga cctcggcgat
     6481 cgcgtcgcgc atgacgcgcg cgccttcgcc cgccgcgccc ttgcgcagcc actccagcag
     6541 accgcgctcg aagcggctgc gtgccgcgcg cagctcccgc gcctcgctcc cggtcgtgtc
     6601 cgccggcggc gccgcacgcg caggcgggcg gcgcgaaaga tcgggatgga agaggtcggc
     6661 ggcgctggcg tcgtgctggc cgcgcgcggc ggcgagctcg aggtagcgcg cggccagccg
     6721 cagcggctgg tcgggcgcgc cgtcggcgag ctcctcgagg tagttgccca ccatcgccag
     6781 cgcgcgcagg gcgagcgcgt ggcgcgcggc gtcggccggg acctcgccgc gcgccatcgc
     6841 cccgagcagc tgctcgaggg cgtcggtgaa cagcgtcagg ccgtcgaggc cgacgatgtc
     6901 gagggcgccg cgcacctggt gcaggtgcga ttgcgcgaac tgcaggccgg cggcttcgct
     6961 gcctgcgcag ccgcgctcga ggatttcgcg cgcgcgcccc agcgcggcgt cgatctcgcc
     7021 cttcacccag gtcagcggtc ccaggtccgg ctcggtggcg tgtgtcatgc tgttcttccg
     7081 ggcgacagcc agcgtggtca gaccttgaag cccgagaccg agcccttcag ctccaccgcc
     7141 agctcggcga gctggtcgat cgacacggcg gtctggcggg tgccgagcga ggtctgttcg
     7201 gtgacggcga gaatgtcctt catcgccgcg gcgacgcggc cggcggtcgc ggcctgttcc
     7261 tgcacgtcgg ccgagatgcg ctcgatcagg ctggcggttt cggtggagac ctggtcgatc
     7321 tcggcgagcg cctggccggc ggcgtcggag agctgcgcgc cctcgaccac gttgcgggtg
     7381 gcgctctcca tcgcacccac cgcgtcctgg gtgtcggtct ggatggtctt gacgatcgcg
     7441 gcgatctgct tggtcgcctc ggccgagcgt tcggcgaggc gctgcacttc ctcggcgacg
     7501 acggtgaagc cgcgcccggc ctcgccggcg gtggcggcct ggatggcggc gttgagcgcg
     7561 agcacgttgg tctgctcggt gatgtcggag atgagctcga cgatctcgcc gatctcctgc
     7621 gaggattcgc cgaggcgctt gatgcgcttg gcggtctcct ggatctggct gcggatgtcg
     7681 ttcatgccgc ggatgctgtt ctgcaccgcg tccgcgccct tgcgcgcgga ctccagcgag
     7741 cgccgcgcca cctgggcgga ctgcagcgcg cgcgcggagg agtcggtcat cgactgcgcc
     7801 atgcgctcgg cggtggcgcc ggcttcctcg atctcctgcg actggcggtc ggtggcggcg
     7861 agcagctcgg tcgaggtacg gcgcgccgac tcggtggccg aggtcacccg cgtggcggcg
     7921 tcgttgattc ggcgtaccag caccgagagc tcctcgatgg tgtagttcac cgagtcggcg
     7981 atcgcgccgg tgatgtcctc ggtgaccgtg gcgcgcaccg tgaggtcgcc gtcggcgagg
     8041 tcgcccatct cgttcatcag gcgcaggatg gcctgctggg tgaggttgcg ctcgtcctcg
     8101 gcctggcggc gctcggcctc cgaggcgagg cggcgcgccg cggcgtcggc ggcgtaggcg
     8161 cgcgccatct gcgccagcac gaacacggcg agcagggcgg cgagcgccgc gcccgcatgc
     8221 agcggaccga agccgccggt gcgcccggcg agcgccgtgc cgagggccgt gctcgcggcg
     8281 agcagggcgg gggtgttcgc cgcgaggccg gcgctcgcgg cgttcgcctg ctgcagcgcg
     8341 cccgcgtcgg cgccgatgcg ctcgatcgcc tggaggatcg gtgccgcggc ggacccgagc
     8401 gcgcgcaggc tctcgtcgag gacgctgccg ttgctcgggc gttcgagttc ggccaggcgc
     8461 tgacgcagct gctcggcctc gccggcgagc accttggccg ccgccgccgg gtctgcatcc
     8521 gcggagaaaa gggtgtttgc ctgcagggtg gcgcgctggg catggagcag gagggcgtgg
     8581 gccgcacgca gcgcagaagc gtctccgttc atcgccgcca cacggcgacc gaactggtcg
     8641 gccgcgtcgg cgagggcgcc gctggccgcg ttgatcgcgc gcacggcagc gctggtgccg
     8701 gcgagcggcg cgccttgggc ggcaaggcgc gccgccagcg tggcgttctt ctgccactcg
     8761 gcgtcgaggt cctgcagcag gggccgcatg ctcgcgggca gcggcggcag cgtggcgccg
     8821 gcatggctgc cgccctggcc gagggtctgc agcaagccgg cgaagcgtgc ccgggtctgc
     8881 tcgagatcgg cgaccgcgcc gggctcgccg aggcgggcga ggcgggccgc cctggccatc
     8941 tgtccgggca gcacctgcag ttcgctcgcc gcgccgagct ggagcgcgga caggctcgca
     9001 tcccgcgcct ggtatgcgag cagtccggcg ctgatcgccg tgcaggcgac gaagagtccg
     9061 ccgagcatgc cgaggcggcc gccggatcga gcggggggag acggagtctt gttggcgacc
     9121 gtctgctccg gcgcggaggt ctcgatgatc gtctgggcct cggtgccggc gccgggggcc
     9181 ggggctttct tgccgaacgg gttcaatgcc atggtcgatg ctcgattttt cggtcgggtt
     9241 cttgcggggt ggatgcgtac gtcattccag cccggcgtcg aggaaggcgg gttgggcgag
     9301 caggcgggcg gggtcgagct gcagccacgc gcggccctgc atgtcgcgca ggcggcgtgc
     9361 cacccagggc ggggcatcgc cgcccggggc gggctcgaag tcctcgtggc tgcgcaggcc
     9421 gctcgaggac gacaccagca gcgcgctgtg gatgccgtgg cgcgcaccga tcagcagcag
     9481 gcgcgcccgc ccgccggcgc tgatcggcgg ctggccgttg aaggcggcga agtcgaccac
     9541 gccgtacagg ctgccgcgca cgttggcgag gccgcggaac caggggcggg tgagcggcac
     9601 cgcggacaag ggcggcgggg gcaggatctc gccggcctcg gcgagctcga tcaaccagtt
     9661 gtcgctgccg cactggaagc cgagcaggcc gcggcgctcg gcgccggcgg cctcggcgag
     9721 gcgacgggcg aggcgttcct ggaattcgcg caggctgctg cgggccatcg agctcagccc
     9781 atcgcccgga tgcgggcgag cagttcgtcg tggtcgaccg gcttgaccag gtagtcgagc
     9841 gcgccctgac gcatgcccca gatcttgtcg gtctcctggc ccttgctggt gcacatgatg
     9901 atcgggatgt cgcgcgtctc gtcatcgcgc gagatcgtgc gcgtggcctg gtagccgttc
     9961 atgcccggca tcaccacgtc catcaggatg aggtcggggt ggtcgctgcg gctgcgtgcg
    10021 atggcctgct cgccgttctc ggcggtcagc acctggtagc cgttgcggcc cagcacctcc
    10081 tggagggcga ggcgttcgac gggagagtcg tcgaccacga gaatcttgcg gatcggcatc
    10141 ggtcgtctcc ggcgtggctc agcctgcgcg cgccgcatcg ccgccgcgcc gtgcgtggct
    10201 ggcgaccgcc cgcaacaggc tgtccttggt gaagggtttg gtgaggtatt cgtcggagcc
    10261 cgccatgcgt ccgcgcgcgc ggtcgaagag gccgtcgcgc gaggacagca tgatcaccgg
    10321 cgtgggcgac aggcgggggt tcttcttgat cagcgcacag gtctggtagc catcgaggcg
    10381 cggcatcatg atgtccacga agatgacgtc ggggtggtgg tcggcgatct tcgcgagtgc
    10441 gtcgaaaccg tcctcggcga ggacgacctc gcagcccgcc tgggcgagga agatctcggc
    10501 gctgcggcgg atcgtgttgc tgtcgtcgat gaccatcacc ttcaggccgg caaggtccat
    10561 gctgtattcc tggtgggttg tcgggcggtg gcgtccgggc ctagatctcg accatctcga
    10621 agtcttcctt gcgcgcgttg cactcgggac aggtccagtt cggcggaacg tcctcccagc
    10681 gcgtgcccgg cgcgatgccc tcgtcgggca ggcccgacgc ttcgtcgtag atgaagccgc
    10741 agatcaggca catgtaggtc ttccaggggg tatcggccat gttccagcgt gactcgaggg
    10801 ttgggggtag aatcgaccgc ctatcttaac gcatccgcgc gcaccccatc cgcgctgcat
    10861 ttccacctcc catgccgctc gccattccgg aaactccccc ctgcgtgctg tgcctgtcgg
    10921 cgggtgacgc cacggccggc ggaggactcg cggccgacat cctgaccctg gcgagcatgg
    10981 gctgccaccc gctgccggtg cagaccgcgg cgctggtgcg cgacacccgc ggcatcgacg
    11041 agctgtggcc ggtcgcgccc gagctgatcg ccatgcaggc gcgcgcggtg ctcgaggacg
    11101 tgccggtggc ggcgttcaag ctcggcttct gcggcagcgt cgagaacgtc gcggtgatcg
    11161 cggagatcct cgccgactat cccgacgttc ccctggtggt cgaacccggc ctgcacgccg
    11221 cggcgctgct cggcgaggcg ggcgaggaga tcgccgcggc gctcgccgac ctgatcctgc
    11281 cgcagaccac gctgttggtg gccgaccgcc acgagctgct gcgcctgacc ggcctggccg
    11341 gggacgaggc ggccgagtcc gacgaggacg acggtggcgg gggaggccag ggcgagacgg
    11401 gtggcgccga gctcgacgag gcgctcgagc gcctgctcgg caacggtacc gaatacgtgc
    11461 tgctcaccgg cggcggcgac cacggcccgc agctcgtcga caccctcttc ggcagcgagg
    11521 gcatcgtgcg caccgacgcc ggcccgcgcc cggccgcgcc ctgcctgggt gccggcacca
    11581 ccctcgccgc cgccgcggtg gcagcgatgg cgcacggcat ggcgctgccc gaggcggtac
    11641 gcgaggcgca ggagtttacc cgccaggccc tgcgccacgc ctatcgcgcc ggcatggggc
    11701 gggcgctgcc cgaccgcttc ttctgggcgc gcggcaaggg cgagagcgat gactgagcgt
    11761 ttcccggcgc gcacgctgcg cgggctctac gcggtcaccc ccgacgaacc cgacaccggc
    11821 cgcctgctcg cgctcgcgca gtccgtgctc gccggtcatc cggcgctgct gcagtaccgc
    11881 aacaagggcg ccgacgccgg tctgcgccgc gagcaggcgc tggcgctgcg cgacgcctgc
    11941 cgggccgccg gcgtgccctt catcgtcaat gacgacctcg cgctcgcgct cgccatcgac
    12001 gccgacggag cgcatctcgg ccgcgaggac ggcccgcccg ccgcggcgcg cgctgcgctc
    12061 ggcccacagc gcatcctcgg tctcacctgc tatgccgact ggtcgcgtgc gaccgagggc
    12121 gccgagatcg gtgccgacta catcgccttc ggcgcgatgt tcccctccac caccaagccc
    12181 cacgcgccgc ccgcaccctt cgcgctcgtc gaacgcgcca ggcgcgagct cggcctgccg
    12241 gtggcggcga tcggcggcat cacgctggag cgtgcgccgc aggtgatcgc cgcgggcgcc
    12301 gacctgctcg cggtggtcgg cgacgtgttc ggcgcggccg atccggcggc gcgtgcgagg
    12361 atgtatcgcg cgctgttctg aaccccgcac ctcccgcccg acgaccctac gcgggcggta
    12421 gcgcctcatc ctggccgagg gcccgcatgc ggggcgatcg cacgaatgca tgacctgccc
    12481 gagcggaacc cgtgggagcg ggcctgcccg cgcaagggcg gcgtgcggcg ctcctgcgcg
    12541 ggcaagcccc ccggtgcaga agatgggcac aggctgcgcc ttcatgcgac tgcgtcgagc
    12601 ttgtatgtgg caatgccagt gctataccgg aatcattcca tgggtctggc ggatgtcatg
    12661 tcgaccgggc gtgagcctcc cgtctcgcga gtccgcatga tggcgggttg atccggcttt
    12721 gccgccttca cggaggctgc acgaatcgca atcgggtcga tggaggtgtc cgtggtgagg
    12781 tgagaaaggg agacggtcat ggatgaagaa aaatggaaaa acctggaggc tctgatcacc
    12841 ggcctcgaca aggtcgtcaa ggctgcagcg gtcaacaccc gaacccgatc actggctgat
    12901 cgcgttaccg ccttgcagag cgatttcgcc tcggccaggg cgagtggcga cgcgaccatg
    12961 cgcgatcaca tcgagatggc ggttcaggag ttgtgggcaa atgtcgagcg ttggagcggc
    13021 gtagcaccga agaggaagat ttcggagtgg ttggcagact tcagcattgc ggggctcatc
    13081 gggctggtcg tgctcctggt tgtactgggc ttgcttgtcg ccgggatcat gttgttcacc
    13141 tccgcagttg gctggaaggc gctcatgggc gtggatggca cacgacccat ccttaccgtg
    13201 gcggcgatca ttgcgacgct ggcgtatggc ggcgggctga ttttcagcgc tctgtactcc
    13261 aacgagggga agttcgaaga acgtttcagg atggcgcgcg aaatctttct ggtgttctcg
    13321 ggggtatttg caaccatcgt cggctttcat ttcggagcgg gcaacaatgg tacgggcggg
    13381 gccggggggg tggaaaatgt gcaacaactc gagcttgatg tccggcgtgg cgcaaagccg
    13441 ggcgagctcg aattgtcggt gacgggggga acgcctccct ataccgtcac aacgacggga
    13501 accaacataa agacaatctc gcccgcgcaa tacgataggt ctcccatcgt gatcggcatc
    13561 acgcgtgaag acccggaaaa atcctggacg gtttcgttct ccgtcgtcga tagggtgggc
    13621 acgaagaagc agatcgagcc gaaacgcgag gtgctcgacg cgcttttcgg ggggcagtcg
    13681 ggcgcaggct ccggggcagg cggaggggga tgaccagacg gtcgccgggt ctcatctcgc
    13741 tgtcgtgcaa ctgtggtcat ccatcgacgt tttcgggctc cgacaagggg cgtcacgtca
    13801 ggctgggcgt gatcggttct ctgtggtcgg cggccaaggc gggcggctca tgcggacgct
    13861 tgccgcgcaa gtcgtgtgaa tgccggtggt ggaccggctt ggccttgcgg cgccacaatg
    13921 ccggctttcg cacgcagcac aggaaccctc tgccatgacc agccgtaacg aagccctctt
    13981 ccagcgcgcc cagcgcagca tccccggtgg cgtaaattcg cccgtccgcg ccttccgctc
    14041 ggtgggcggc acgccgcgct tccttgcgcg cgccgaaggt gcccgggtgt gggatgcgga
    14101 tggcaaggag tacatcgact acgtcggctc ctggggcccg gcgatcgccg gccacgccca
    14161 cccggcgatc gtcgaggcgg tgcgcgaggc ggcgctgaag ggcctgtcct tcggtgcgcc
    14221 caccgagagc gaggtcgaca tggccgagct gatctgcgcc atgctgccct cggtggagat
    14281 ggtgcgcctg gtcagttcgg gcaccgaggc gaccatgagc gcgatccggc tggcgcgcgg
    14341 cttcaccggc cgcgatgcga tcgtgaagtt cgagggctgc taccacggcc acgccgacag
    14401 cctgctggtg aaggccggct ccggcctgct gaccttcggc aacccgtcct cgggcggcgt
    14461 gccggcggac ttcgccaagc acaccatcgt gctcgactac aacgacctgc agcaggtcga
    14521 ggacgtgttc aaggcgcgcg gcgacgagat cgccgcgatc atcgtcgagc cggtggccgg
    14581 caacatgaac ctgatcaagc cgcagcccgg cttcctcgaa ggcctgcgcc gcatctgcac
    14641 cgagtacggc gcggtgctga tcttcgacga ggtgatgacg gggttccgcg tcggcccgca
    14701 gggcgtgcag ggcctctacg gcatcacccc cgacctgacc acgctcggca aggtgatcgg
    14761 cggcggcatg ccggtgggcg ccttcggcgg tcgtcgcgac atcatggaaa agatcgcccc
    14821 gctgggcagc gtttaccagg ccggcaccct gtcgggcagc ccggtggcag tggcggcggg
    14881 catggtgtcg ctgcagctga cgcgtgaggc cggcttctac gagcggctga cggcgagcac
    14941 gcagcgactg gttgcgggtc tcgccgcggc ggccaaggat gccggcgtca cgttcagcgc
    15001 ggactcggtc ggcggcatgt tcggcgtcta tttcgccgac aaggtgccgg cctccttcgc
    15061 ccaggtgatg gctacggaca aggagcgctt caaccgcttc ttccacgcca tgctggaagc
    15121 cggccactac ttcgcgccct cggccttcga ggccggcttc gtgtcggtgg cgcatggcga
    15181 ggccgagatc gacgccacgg tggccgtggc gcgcgcggtc ttcgcccaac tgggctgagc
    15241 cttcccgcag gcctgcggag cgggctcgcc cgctcctggc gccatattcc tcacatctcg
    15301 cccgtcagag caggaaaagc gtcgccagcc cgaggaagat gaagaagccg cccgagtcgg
    15361 tgagtgcggt gatcatcacc gagccgccga gcgcggggtc ggcgccgagg cgctggcgca
    15421 gcatcgggac cagcacgccc gcgcaggctg ccagcaggag gttcagcgtc atcgcaccgg
    15481 tcatcaccag gccgagatcg acgttgccgt agagccacca cgccagcacg ccgagcagtc
    15541 cgccccacac cacgccgttg atcagcgcca cgccgagctc cttgcgcagc aggcgggtca
    15601 tcgccgaggg ctcgacctgg cccatcgcga tcgcacgcac gatcatcgtc acggtctggt
    15661 tgcccgagtt gccgccgatg ccggcgacga tcggcatcag cgccgcgagc gcgaccagct
    15721 tctcgatcga ctcctcgaac aggccgatca cgcgcgaggc cacgaaggcg gtgacgaggt
    15781 tgatcgccag ccaggcccag cggttcttca ccgagctcca caccgaggcg aagatgtcct
    15841 cctcctcgcg caggccggcc tgggacagga tctcggcctc ggactcctcg cggatgaagt
    15901 ccaccgcgtc ggccacggtg atgcggccga tcacccgccg ctcggaatcg atcaccggcg
    15961 ccgacaccag gtcgtagcgc tcgaaggcct gggcggcgtc gtcggcctcg tcctcggggg
    16021 tgaagctgat caccggctcg gagcgcatca cctcggccac ggtcttttcc gggtcgctga
    16081 tcagcagcgt ctccagggtc aggatgccct cgaggtggtc ctcgcggtcg accacgaaga
    16141 gcttgtcggt gtggtcgggg agttcgtcga agcggcgcag gtagcgcagc acgacctcca
    16201 gggtgacgtc gtcgcgtacc gtcaccatgt cgaagtccat cagcgcgccg acggaatcct
    16261 ccgggtagga catcgccgcg cgcagctgct cgcggccctc gctgtcgagc gactggaaga
    16321 cgtcctcgat gacctcgggc ggcaggtcgg gggcgaggtc ggcgagctcg tcggcatcca
    16381 gcgaacctgc cgccgccttg agctcctcgg ggtccatcgt ctcgatgagc gactcgcgta
    16441 ccgcgtcgga gacctcgagc aggatctcgc cgtcacgctc tgccttgacc aggtcccaga
    16501 catagagacg ctcctcgcgc ggcagcgcct cgaggatgta ggcgatgtcg gccgggtgca
    16561 gactgtcgag cttggcgcgc agcccggcct cgtgctgctt gtgcacgagg ttctccacca
    16621 gctcgtggcg cggcatgtcc tggcgatgga cgagaccctc caccaccttc tggcgggcga
    16681 gcagatcctg cacctccttg aggtgctgct ggacgtcgtc ggcgtggagt tcttcgggct
    16741 cggtcatcgc gcgcaaggcc ggcggagggg caagtcgcga attctacgga ctcccccccg
    16801 cgcacgcgac cccgatcatg tgacagaagg cgaaaaatcg gcggagggcc attcccgccc
    16861 ctcgcgcctt gcgcgtcggg ctcaaggcac ctgcgaattc gccatccgcc gctgcacccg
    16921 cgccgcgtgc gcggccaggc gggggccgaa ggtcttctcg tagcgccgcg ggttgggcag
    16981 catcaccgcc aggcgcgcgg cctcggccgg gcccaggctg gcggccgggc ggccgtagta
    17041 gtggcgcgcg gcggcctcgg caccgaacac gccgttgccc cattcggcca cgttcaggta
    17101 cacctcgagg atgcggcgct tgtcccaggt cgcctcgatc atcagggtga tcgccgcctc
    17161 ctggcccttg cgcagccagg agcgcgagga cgacaggaag aggttcttgg cgagctgctg
    17221 gctgatcgtc gagccgccgg cggtgacgcg gccctcctcg gcgttgcgct ccagggcgcg
    17281 ctcgatgccc tcccagtcga agccgtcgtg gtcgaggaag cgatcgtcct cggcagcgac
    17341 taccgcgcgc ttgaggtgga tcgagatgcg ctcgtagggc acccattgct ggcgcagttc
    17401 ggcccgcggg tcggcttcgc gcaactcggc gaggcggatg cgcatgaagc tcgtgctgcc
    17461 gggatcgaag cggctccacc acaccacctg ggtcagcagc accgcctgcc agaacagcgc
    17521 caacagcagc gcgacaccca gcccgcgggc gacccagccg aggatgcggc gcggcgcggg
    17581 gatcggcacg ctggcctcag ccggcttcga tgagctggcg cagctcggcc agcaccggcg
    17641 cggagtcggg gcgcacgccg cgccacagcg cgaagctctc ggcggcctgc tcgaccagca
    17701 tgcccagccc gtcggccacc cgcgccgcac cgtcggcgag cgcggcgcgc aggaagggcg
    17761 tgtggccgcg gccgtacatc atgtcgtagg cgagcgcgcc ctgggcgtag aggccgggcg
    17821 gcagcggcgg cagttcgccg gacaggctgg cggaggtggc gttgatcacg aggtcgaagc
    17881 gctggtcggc gagcgcctcg aagccgcagc cctcgatgcg caccgtgccg gcgtgggcgg
    17941 cgaaggcctg cgccagctcc accgccttgt gcgccgtgcg gttggcgatg cacaggtcag
    18001 cgggtgcggt gtcgagcagc ggcaacagcg caccgcgggt ggcgccgccg gcgcccagca
    18061 gcaggatgcg ccgcccgcgg ggcgggcaac cgagatggct cgcgaggtcg cgcaccaggc
    18121 cggcgccgtc ggtgttgtcg ccgaggatgc cgtccgtgcc gaaggcgagc gtgttgaccg
    18181 caccggccgc ctcggcacgc ggcgtgcggc ggtcggcgag ggcgaaggcc tcgagcttga
    18241 agggcacggt gacgttgagg ccgcgcccac cctcggcgcg aaagcgcgcc accgtcgcgg
    18301 cgaaatcgtc gagcggggcg agcagggcct cgtaggtgag cgcctgcccg gtctggcgag
    18361 cgaacgcggc gtggatccag ggcgacttgc tgtgggcgat cgggttgccg acgacggcgt
    18421 aacggtccat gggcggggtc tcagttggcg cgcacctggt cgctgttggt gaaggtccac
    18481 gtgcgggtga tctcgatcac gtcgtagtcc ttgcggatat cgggtgggaa gggggcgtag
    18541 ggcgcggcca tcttgacgat gcgcagcgcg gcgtcgtcga gcaccttgtg gcccgaactg
    18601 cgatgcacgc ccatccgctc caccgtgccg tcggagcgga tcgtcaccga cagcagcagg
    18661 ctgccgtaga gccggccgcg cgccgcatcg gggtagttca gcgtgccgat gcgctcgacc
    18721 ttctgccgcc agtcctcgac gtactgcgcg aagcggtatt cctgcgcgcg cgcgccgatg
    18781 aacttcttgc gcggacgctt ggcgagttcc ttgaggtcgc ggtcgatctg ggcctcgatg
    18841 cgggcgaccg cggcggcgct gtcgagcagg tcgaggccgc tgatctcgcg ctgcggctcg
    18901 ggtgtcggcg tgggctgctc ggccttcttg ggcgccgcgg cgaccttggc aggggccttg
    18961 tcgcgagtga tcacctgctt ctgttcgcgc accggctcgg gcgtgcggcg cttggcctcg
    19021 accagggcgt cgccgtcctg gcggttgggc tgcggtggca gcggtgtgct cgcgcgcacc
    19081 tcctgctcgg tgttgccgcc ggcatcgacg ttggcctgcg cgagcacctc ggccttgtcg
    19141 ggtgcccggg cgtggcgggc gttgaccagc accacttcca gacccttgtc gcgcgcgggc
    19201 ttggcctcgg gcaggcggaa gtggatcagc agcagcagcg cgtgcaggct gatcgagatg
    19261 acgaaggccg ccaccagcag cggtccgccg aagcgccgca ggcgcccgct ccaggccccc
    19321 ggcagggcgc cggcgggcag gacgggggcg gcgggcggag tcatgcgcag atcacgcgaa
    19381 accgaagccg gcctcgtcca ccgtctcggg atcgggttcg gcgagcgtct gcacgaagcg
    19441 tgcctccacc tcgacgtcga gcaggtcggt gcgctcgacg acgagcttga cccggctgcc
    19501 gggcatctgc agcggcatcg agggtacgcg gatcaccagc ggaatctcgg cgaggcgcac
    19561 cacgttctcg cgcagcaccg tggcctcgac ctcgccgcgg ccgtgctggc gcagccagcg
    19621 caggcaccag tagcgctcca tgccgcgctg gaactcggcg tactcggcgt aggtgagctc
    19681 gaagtcgcgc atcgccgcca tcagctcggc cgacttgggc gcgaacgcgg gcggggtgcc
    19741 ctgcagcacc gagatgatct gccactggtt caccaagtcg acgtagcgcc gcagcggcga
    19801 gctcgaccac gcgtagcagt ccaccccgag gccttcgtgc ggcgcggcga ccgtggtcat
    19861 gcgcaccttg ccgccgccct gggcgcgata caggccgggg atgcccgagg tatcgagcag
    19921 cttgccccag gtcatgttgg cgtggatcat cagctcggcg acgagcttgt ccatcggcga
    19981 gccgcgcagg cgccggccga tgctgacgcg tcccgggccg tccgcggtct cctcggtcca
    20041 gtccacgctg aagccgaagt cgatgcggtc ctcgttgccg gcggccttgc cgcgtccagc
    20101 ctcgagcacg gtggcgaggt cccacagcag ggtcagttcg cgcttccact ggaagtcggg
    20161 gccgccgttg tggacggtgt cgtcgttgaa caccggctcg atgtcgtggt ggcgcaggtt
    20221 ggccacgatc ggcacgagct cgacccggct ctcctggtgg acgatcgcca gccccggggt
    20281 gatgtcgagg tagagcgaca ccgccggaca gtcgcggccc tcgccgaggg tgaaggtctg
    20341 cacgatctcg tcgggcagca tggtgatctt gttgcccggc atgtagacgg tggacaggcg
    20401 ccggcgcgcg accgcgtcga gcgtcgagcc gcgcgcgaag ccgagcgcgg gtgcggcgat
    20461 gtggatgccg atgcgccagc cgccctcggc gcgcggcgtc accgaaaagg cgtcgtcgat
    20521 ctcggtggtg gaggcgtcgt cgatcgagaa tgcggcaacc tcggcacgcg gcagcgcggt
    20581 cggcagcacg gggggctcga tggcggggaa ggcggtgcct tcggggaagt gctcgaagag
    20641 gaagcggttg taatggaagt cgtagctcga ggcgagcgca ccgcaggcca gcagcaggcg
    20701 cgcggccgac agcccgctgt cgacgcaggc ggcttcgagc gccttgatct ctgggcggtt
    20761 gcggtcgggg cggtagagcg cctgcggcag cagcgcggcg agctcgggcg gcaggcgccc
    20821 ggcgaccagt tcggcgcgca tgtgctcgat cgcggcggcc tgctggcgct ttttctcgag
    20881 gccggcgagc gcggcctgga ggatgtcggc gggggccttg cggaagcgcc cgcgtccctt
    20941 gcggtggaac cagatcggcg ccgagtgcag gcgcagcagc agggcggccg attcgaccgc
    21001 gttcggcaca tggccgtgat attcggcggc gaagtcggcg aaggcgaact cgtcgtcgcc
    21061 gcacacttcc cagaggaatt cgacgtccag gccttcggcc tcggcctcgg cgcgcgcgag
    21121 caggtcggaa ggccccggct cgcggaactc catcagcaca tgggcgcgct tgagcttcac
    21181 ccgcttgccg tgggtgttct cgacctgcag cgtggcgtcg ttatcggcga ggatggttcc
    21241 ggccttgaag gccccgtcct cttcatacag cacgaacatc ggcatccgcc tgaagaaagc
    21301 aagcgcgcta gtttactgga ttcgggccgc cggccctatc gcggggtgcg aatgaggggg
    21361 cgatgccgcg cggggggcga tccggagcgg ctccggtcgg ggctggcacc gattcggtgc
    21421 ggctccagtg cggtgcggtt cggcgtcggc gtggttcgga gtcggtgcgg cttcgattcg
    21481 gccgcggggc cggggtggcg ggggccgcct gcactcgcg
//
LOCUS       DAOQBS010000046        20805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1746_length_20805_cov_14.425108, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000046 DAOQBS010000000
VERSION     DAOQBS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 20805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20805
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1746_length_20805_cov_14.425108"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1390)
                     /gene="rlmD"
                     /locus_tag="PKN25_07130"
     CDS             complement(65..1390)
                     /gene="rlmD"
                     /locus_tag="PKN25_07130"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNV90707.1"
                     /translation="MPTAKIDSLDHEGRGVTRVEGKAVFVEGALPGERVEYVVRRAKP
                     SYEQGEVVRVLRPSAQRVAPRCPHYGVCGGCSMQHLDADAQAAVKQRLLEDALRHIGK
                     LHADRILPAIHGPAWGYRYRARLGVRLVPSKGGLRVGFHERRSSYIVDMRTCPVLPPA
                     ISALLPRLRTMLAGLSIAERLPQVEIAVGEDRTVFVFRNLLPFSAADEKQLAAFADAE
                     GIQAWVQPAGPDSARPLHPANAQLPFYTLPEFGLRLDFRPTDFTQVNADINRLLIRRS
                     MQLLDPRPGERIADLFCGLGNFSLPIARLGAEVVGVEGSEALVERAHANAARNGLAAR
                     CEFHAANLFETTEDSLAALGRLDKLLIDPPREGAIAVVKAIDPSRGPSRIVYVSCNPA
                     TLARDAAVLVHEKGYRLAAAGIANMFPQTSHVESVALFERTPGTEVGPG"
     gene            1634..2059
                     /locus_tag="PKN25_07135"
     CDS             1634..2059
                     /locus_tag="PKN25_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90708.1"
                     /translation="MKHSILAAALVALAVSACGKKEEPVAVQPAPSAQEQMKESAAQA
                     VEAAKESAKAAGDAAAAGAQAVKQEAAAAVEATKEAAATAAAKADAAADAAAKKADAA
                     VEAVKDAAADAKADTVDAAAQAKAAAEAAVGAAKESAAK"
     gene            complement(2194..3336)
                     /gene="bamC"
                     /locus_tag="PKN25_07140"
     CDS             complement(2194..3336)
                     /gene="bamC"
                     /locus_tag="PKN25_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNV90709.1"
                     /translation="MIRSKRTSLSLLALSIALGGCSGSLLESKRIDYKSAKQIERPLE
                     IPPDLTAPQRDDRFAVPDVAPRGVATYSAYTADRAGQPAQATSGPLVLAKSDTMRIER
                     AGSQRWLVVGGTPEELWPQIKDFWLELGFILNIDSPEIGVMETDWAEDRAKIPQDFLR
                     SSIGKVLDGLFSTPERDKFRTRLEQGKETGTVEVYISHRGMMEIYPTEAKDSTIWQPR
                     PADPELEAEMLRRLMVRLGAEEARAEVAIATPQQAERARIASAPDGQVMLVMDEAFDR
                     AWRRVGLALDRIGFTVEDRDRAQGLYFVRYVDPDADNRSTEKGFLSRLAFWRSDAKPA
                     AGGSEYRLRVQGQGEASRVSVLTREGGLDNSDTARRMLGLLREQLR"
     gene            complement(3363..4241)
                     /gene="dapA"
                     /locus_tag="PKN25_07145"
     CDS             complement(3363..4241)
                     /gene="dapA"
                     /locus_tag="PKN25_07145"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNV90710.1"
                     /translation="MITGSLVAIVTPMHDDGSLDFPRLRSLIDWHIAEGTDGIVIVGT
                     TGESPTVTVDEHCELIRVAVEHCAGRVPVIAGTGANSTAEAVELARFAAQAGANAHLS
                     VVPYYNRPTQEGLYRHFRTIAEAVELPLILYNVPGRTVADLANDTVLRLAEVPNIVGL
                     KDATGNLDRACDLVERAPAEFALYSGDDMTSAAFLMLGGHGVISVTANVAPRAMHALC
                     AAAAAGDMRTLRATNAGLTGLHRDLFCEANPIPVKWAVARMGLIGDGLRLPLTPLSAA
                     HHERVRAAMRKAGIQA"
     gene            complement(4384..5310)
                     /locus_tag="PKN25_07150"
     CDS             complement(4384..5310)
                     /locus_tag="PKN25_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNV90711.1"
                     /translation="MTTTAFITHRDCWLHDMGAMHPECPDRLSAINDRLIASGLDMYV
                     SFFDAPLAEREHILRVHPAEHLDNLIESVPEHGIRHLDPDTAMSPGTMKAALRSAGAG
                     VYATDLVMKGEIENAFCAVRPPGHHAERAKAMGFCFMNNVAIAARHALEAHGLERVAV
                     VDFDVHHGNGTEDIFRNDPRVMMAGIFQHPFYPYCGTENPPAHMCNVPLPAGTRGDAF
                     RQVFGDIVVPALRSHSPQMIFISAGFDAHYEDDMGSLGLLEADYIWATQQIKGVAESC
                     GHKRIVSILEGGYSLSSLARSVVAHIKTLADL"
     gene            5464..6381
                     /locus_tag="PKN25_07155"
     CDS             5464..6381
                     /locus_tag="PKN25_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNV90712.1"
                     /translation="MTLHHANRLLEAASRIILGKERELRLALACLIARGHLLIEDVPG
                     VGKTTLAHALARLMGLQFQRIQFTSDLLPADILGVSIFDRNTAAFRFHPGPVFAQLIL
                     ADEINRATPKTQSALLEAMEERQVTADGQTCALPEPFFVIATQNPAHQIGTFPLPESQ
                     LDRFLLRIRLGYPDPQAERALLMGEDRRGLLERQPAVIGPGELLDMQLAAQGLHVAEA
                     LVAYVQTLLAATRNSAELAGGLSPRAGLGLLAVARAWALLDGRDHVLPEDVQTLFPHV
                     AAHRLHLAGDGRPAPAATLVRLLQTVAVG"
     gene            6381..7382
                     /locus_tag="PKN25_07160"
     CDS             6381..7382
                     /locus_tag="PKN25_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNV90713.1"
                     /translation="MARTAASLATRLRHAGERWLFRIGAPEPAPIRLGQRRIYVLPSA
                     AGVGFALALLVMLLAAINYNLSLGYALVFTLGGSAAASILHAFRNLYGLSIRPGRCAP
                     VFAGETAVFSLLIDNPASRRRPALRLGAQGQWSAFDLPAAEETAVALACPALRRGRLA
                     LGRSVLETRWPLGLIRAWSVFVPKADCLVLPTPEADPPPLPPAGGDATGASRDAHTGD
                     EDFAGLRAHRTADSPRHVAWKAVARGGPMLTKEFAAGEGRELLLDWDRLPAGLDAERR
                     LSRLTAWVLAAEREGRRYALALPGIRIAAATGRAHRERCLRLLALHGLPDPTAEDGA"
     gene            7379..9397
                     /locus_tag="PKN25_07165"
     CDS             7379..9397
                     /locus_tag="PKN25_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HNV90714.1"
                     /translation="MSVARARRLIGLRRRGGPLGGTRTGPLAPALRRDQGAWLLTAAA
                     MAIAPHAGWLPGWIGALGLGLLGWRALLLWRGSRPPPGTLLFALALAVAAGVRLEFGH
                     FFGREPGVAVLVLLLGLKLLETRAARDIRAGVLLCLFLQLALFFEDQSLPVAALVLAG
                     TLPALGALVALADPDGGERERLLTAATLLAQGLPFMLALFVLFPRIQGPLWGLPADAF
                     SARTGLSDTMRPGSISALGESDEIALRAAFSGPPPPPAQRYWRGPVLTRFDGREWHAE
                     PAAESASPSYTPQGERIDYLLTIEPHQRRWLIALEHPGPARPPIRYTGDLRALAAEPL
                     RARARFALGAYPHTPVGVAESPAVLASATTLPADSNPRSRRLAAELAAGARDHAEILE
                     RVLARLRALRLAYTLRPPLLGRHAADEFLFDARRGFCEHFASAFAVLMRAAGVPTRIV
                     TGYQGGEINPIDGQLVVRQSDAHAWTEVWLQGRGWLRVDPTALAAPERIDGGLAAALA
                     DAGELPLMLRADMAWLRGLRHRWEAVANLWNQHVLGYNPERQRELLARIGLGADRLAQ
                     TLGVLAGAAVLLFAALYAWSLRATRVRDPLARTWERFCAKMAAAGAARPAWQGPQDYA
                     EELATHFPAHAMELRGICMLYARLRYGPPAPEQQLRILHNRIASLRLK"
     gene            9394..10476
                     /gene="mltB"
                     /locus_tag="PKN25_07170"
     CDS             9394..10476
                     /gene="mltB"
                     /locus_tag="PKN25_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HNV90715.1"
                     /translation="MIPTPSLPARPSSPTQRPPYRQRKLGGILAAFAIALCTPALAHA
                     SYAEREDARAFAAEIASRHALDAGEIERALAGARHDPEVIRLITPPSRKGVRSWRSYR
                     ARFLDRTRIDGGMRFWHEHADTLERAERETGVPAEVIVAIIGVETIYGRNTGNFETLS
                     TLATLAFDYPPRAELFRRELEALFLLAREQGRDPASYYGSFAGALGYPQFLPSSMRAY
                     AVDFDRNGAIDFEGDPVDAIGSVANYLRAHGWQAGAPVAERARLSPATDAAGLVAAGI
                     EPTLAPALLADNGITTADGRPVAAAATLVDLETPGADTEYWLGYRNFYVITRYNRSSF
                     YAMSVFELAEALRLGRLVEEIRAERR"
     gene            10488..11273
                     /locus_tag="PKN25_07175"
     CDS             10488..11273
                     /locus_tag="PKN25_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HNV90716.1"
                     /translation="MPASAPPSSSDPSSLRPAPLGLADLGGALRFGITQFRAIPLLSM
                     AFALLFVVIGALLFGILEFGAIAPMSLSLAGGFMLVGPVLLAGFFGVSDKLRKGRQPS
                     FGDIWSGFRQMPRGGWVVSFVCGLLFLIWITDAGILYGFMVGREPIGFVRLLRIEQVV
                     MDYAGYSAIMGGVLAFILFAVSAFSIPLIYDGRATLVSGVVASVRAVFGRFGVMMCWA
                     FLLAAVIMGSVMALPLLLVSLPVMAYASRELYFRTFPPAAPAT"
     gene            complement(11308..12246)
                     /gene="rpoS"
                     /locus_tag="PKN25_07180"
     CDS             complement(11308..12246)
                     /gene="rpoS"
                     /locus_tag="PKN25_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HNV90717.1"
                     /translation="MEEPVNLDELESQQEPDLPPEVEVFSFQAPPVVENEFFSDVTQL
                     YLNEIGANPLLTAEEELVIARRVRMGDFDARQTMIERNLRLVVNIAKHYLNRGIPLLD
                     LVEEGNLGLMHALEKFDPERGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHVVKELN
                     QVLRAQRHIEADCNGESSLEQIANRLGKTIEEVRSILALGEHTASLDAPLDIDPSLSI
                     GESLADEQQISADLRIQCSEVEQLVREWLAILNDKQRMVIRHRYGIDECELLTLEELA
                     ERLELTRERVRQIQLEALGQLRRILRRRGISRDALL"
     gene            complement(12258..13082)
                     /locus_tag="PKN25_07185"
     CDS             complement(12258..13082)
                     /locus_tag="PKN25_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNV90718.1"
                     /translation="MHKNTSHLIALVAAALLAGCASQGTAPVRDGSGVSPAPAAAVAP
                     ASGYHTVRPGDTLLGIARQYGVTLPDLVSWNRITDPDQIHVGQSLRVSPSASTGSIAG
                     AGGAIATPIPSTGSATVPTVREPVGGRAPVTEPAAVESPPPAAAEAPPSTMSGQWRWP
                     ASGSVIAGFNEASNKGLDIGGAVGEPVYAAAAGKVVYAGSGLRGYGKLIVIKHNQEYN
                     SVYAHNDKLLVKEDDQVAQGQKIAELGSSEADRPKLHFEIRKQGKAVDPAGYLPAR"
     gene            complement(13099..13752)
                     /locus_tag="PKN25_07190"
     CDS             complement(13099..13752)
                     /locus_tag="PKN25_07190"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNV90719.1"
                     /translation="MSVRASDPGQASRARARMVERLRAQGIRDERVLAAMQAVPRHAF
                     VDEGLAFSAYDDTPLPIGFQQTISQPFVVARMLELLRNGRELGRTLEVGAGCGYQAAV
                     LSLLATEVYAVERIRGLLDRARANLRPLRLPNVRLKLADGSLGLPEAAPFDSIVVAAA
                     AVGLPPALKEQLASGGRLIIPVGGAEQRLLLIERQGNTFRETWCEAVRFVPLIAGTD"
     gene            complement(13749..14492)
                     /gene="surE"
                     /locus_tag="PKN25_07195"
     CDS             complement(13749..14492)
                     /gene="surE"
                     /locus_tag="PKN25_07195"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNV90720.1"
                     /translation="MRILVSNDDGYFAPGIAALAEALGTIGQVTVVAPERDRSGASNS
                     LTLDRPLSLRQTSNGFYHVNGTPTDCVHLAVTGMLDHLPDMVVSGVNHGANMGDDTVY
                     SGTVAAATEGFLLGVPSIAVSLVSKSATDFTAAARVARDLAERFMRNPFPRPVLLNVN
                     VPDVAYERLQGIRVTRLGKRHKAEPVVRSETPRGETVYWVGAAGGAADAGEGTDFHAV
                     ANGFVSVTPLQIDLTHTGLIAPVFDWLQR"
     gene            complement(14644..14720)
                     /locus_tag="PKN25_07200"
     tRNA            complement(14644..14720)
                     /locus_tag="PKN25_07200"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14684..14686),aa:Pro,seq:ggg)
     gene            complement(14805..15185)
                     /locus_tag="PKN25_07205"
     CDS             complement(14805..15185)
                     /locus_tag="PKN25_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNV90721.1"
                     /translation="MQASSPETGGEQLPPIPAKRYFTIGEVSELCCVKPHVLRYWEQE
                     FTQLKPVKRGGNRRYYQHHEVMLIRRIRQLLYQEGFTISGARNRLGESAIQEQENGVE
                     LEQCRSTLRQLRAEIAATLQELRA"
     gene            complement(15166..15471)
                     /locus_tag="PKN25_07210"
     CDS             complement(15166..15471)
                     /locus_tag="PKN25_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNV90722.1"
                     /translation="MTLTKAELADLLFEQVGLNKREAKDMVEGFFEEIRTALERGDSV
                     KLSGFGNFQLRDKPQRPGRNPKTGEEIPITARRVVTFHASQKLKAAVEQLSDASKQP"
     gene            complement(15473..17863)
                     /gene="pheT"
                     /locus_tag="PKN25_07215"
     CDS             complement(15473..17863)
                     /gene="pheT"
                     /locus_tag="PKN25_07215"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNV90723.1"
                     /translation="MQFSEQWLRTFVDPQLDSAGLGHLLTMAGLEVEEAEPAAPAFRG
                     VVVAKIVEAEKHPNADKLKLCKVDAGTGELLQIVCGAPNAAAGMKVPCAVVGAVLPGE
                     FEIKKAKLRGVESFGMLCSARELGFSEDHSGLYALPEDAPVGTDVRAYLGLDDTLFTI
                     KLTPNRADCLSLTGVAREVAAITGGEFRPLAIDPVAPAIEARRDIVLDAPQACPRYCG
                     RILRGVDARAATPDWMVRRLVRSGIRSISALVDITNYVMLELGQPLHAFDNAKLQGAI
                     HVRLPRAGEQVLLLNEQTVTPAADTLLIADEARALALAGIMGGEDSGITLDTTEVFLE
                     SAFFAPDAIAGRARAYGFGSDASHRFERGVDPELARSALERATRLVIELCGGEAGPVV
                     EAVATDALPARAPVRLRPARARRVLGIVLADADMLALLRRVHLQVERTGDDILVTPPS
                     WRFDIEIEEDLIEEIARLHGYDNIPAIAPQGSLMMLPSSENGRPVWALRHLLAARGYQ
                     EVVNFAFVEEAWERDFCANENPIRLANPIASHLSVMRSSLIPGLVGTLAANRKRQIDR
                     VRVFEVARVFERKADGEPVRGFHQVMNIAALAAGQVVPEQWGERARSVDFYDVKGDLE
                     ALFAPRELQFAPLSHPALHPGRAAAVRLDGHMIGVLGELHPVWVQRYELGTAPVVFEV
                     ALDAALAARVPAYAEISRMQAVRRDLALVVGNEVPVERVLEVLREAAPALVREIALFD
                     LYQGKGIDSDKKSLAFKVSMQDTRRTLEDAEVDAAVALLVTHAETVLGARLRGAGG"
     gene            complement(17913..18944)
                     /gene="pheS"
                     /locus_tag="PKN25_07220"
     CDS             complement(17913..18944)
                     /gene="pheS"
                     /locus_tag="PKN25_07220"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNV90724.1"
                     /translation="MEQLDQLVQQAAAEFAAAVDGAQLEQAKARYLGKSGALTERLKA
                     LGKLEPEARREAGAAINAAKTAIEAALEGRRNALREAALLAQLAAEALDVTLPGRGGV
                     QGGLHPVSRTLERVERLFGSIGFVVADGPEIETDWHNFTALNTPENHPARSMHDTFYL
                     EGHDDVLLRTHTSPVQIRAMQAHVARHRDADAMPDLRIIAPGRVFRVDSDATHSPMFH
                     QVEGLWVGETVSFADLKGVIADFLRRFFETDDLQVRFRPSFFPFTEPSAEIDVAFMSG
                     ALEGRWLEIAGCGVVHPNVLRFGGIDPERYTGFAFGMGPDRLTMLRYGVNDLRLFFEG
                     DLRFLGQFR"
     gene            complement(19100..19459)
                     /gene="rplT"
                     /locus_tag="PKN25_07225"
     CDS             complement(19100..19459)
                     /gene="rplT"
                     /locus_tag="PKN25_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNV90725.1"
                     /translation="MPRVKRGVTARARHKKVLSQAKGYRGRRKNVYRIAKQAVMKAGQ
                     YAYRDRRQRKRQFRTLWIARINAAARELGLTYSVFMNGLKKAAIEIDRKVLADLAVFD
                     KPAFAAIAEQARAKLAA"
     gene            complement(19472..19669)
                     /gene="rpmI"
                     /locus_tag="PKN25_07230"
     CDS             complement(19472..19669)
                     /gene="rpmI"
                     /locus_tag="PKN25_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNV90726.1"
                     /translation="MPKMKTKSGAKKRFKVRSSGGIKRSQAFKRHILTKKTTKVKRHL
                     RGMTEVHAADEKLIRAMLPYA"
     gene            complement(19815..20336)
                     /gene="infC"
                     /locus_tag="PKN25_07235"
     CDS             complement(19815..20336)
                     /gene="infC"
                     /locus_tag="PKN25_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNV90727.1"
                     /translation="MAQEKKQRVNREITAPEVRLVGEEGEQLGIVSLNASLSMAEEAG
                     LDLVEIAPMAVPPVCKLMDFGKFKYQEQKKAHEARLKQKQVQVKEVKLRPGTDENDYQ
                     IKLRNLKRFLEEGDKCKVTLRFRGREMAHQEFGLRQLERVKADLEELGQVEQMPKMEG
                     RQMIMIIAPKKKH"
     gene            complement(20363..>20805)
                     /locus_tag="PKN25_07240"
     CDS             complement(20363..>20805)
                     /locus_tag="PKN25_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNV90728.1"
                     /translation="GAEYVDEDNTKKIPVMLHRAILGSLERFIGILIEHHAGAMPLWL
                     APVQAVVMNISEGQAEYAEEAVARLKKAGFRVEADLRNEKINYKIREHSVQKLPYQIV
                     IGEKEKAAGLVAVRARGGQDLGQMSLDSLIERWLREVEAKAGSV"
BASE COUNT         3097 a         7270 c         7099 g         3339 t
ORIGIN      
        1 gcttccgcaa gactgcaaag tataccaccg cgacgcaccc gcatcggagg gcgagcgccc
       61 ccgctcagcc cggcccgact tcagtccccg gggtgcgctc gaagagcgcc acggactcga
      121 cgtgcgaggt ctgcgggaac atgttggcga tccccgccgc ggcgagccga tagcccttct
      181 cgtgcaccag caccgcggcg tcgcgcgcca gggtcgccgg gttgcacgac acatacacga
      241 tccgcgatgg cccgcgtgac gggtcgatcg ccttcaccac cgcgatcgcg ccctcgcgcg
      301 gcggatcgat caacagcttg tcgagccggc cgagcgcggc caggctgtcc tcggtggtct
      361 cgaacagatt cgcggcgtgg aactcacagc gcgcagcgag cccattgcgc gccgcgttgg
      421 catgggcgcg ctcgaccagg gcctcgctgc cctccacacc caccacctcg gcgccgaggc
      481 gcgcgatcgg caggctgaag tttcccagcc cgcagaacag gtctgcgata cgctcgcccg
      541 ggcgcgggtc gagcagttgc atcgaacgcc ggatcagcag tcgattgatg tctgcgttca
      601 cctgggtgaa gtcggtaggg cggaaatcca gccgcaggcc gaactcgggc aaggtgtaga
      661 acggtagctg cgcgttcgcc gggtgcagag gccgcgccga atcggggccc gcgggctgga
      721 cccaggcctg gatgccctcg gcatcggcga aggcggcgag ctgcttctcg tccgccgcgg
      781 agaacggcag caggttgcga aacacgaaca cggtgcggtc ctcgcccacc gcgatctcga
      841 cctgaggcag gcgctccgcg atcgacagcc cggccagcat cgtgcgcagg cgcggcagca
      901 gcgccgagat cgccggcggc agcaccgggc aggtgcgcat gtcgacgatg tagctcgagc
      961 ggcgctcgtg aaaacccacg cgcagcccac ccttgctcgg caccaggcgc acgcccaggc
     1021 gcgcgcggta gcggtagccc caggcgggac cgtggatcgc cggcaggatg cggtcggcgt
     1081 gcagcttgcc gatgtggcgc agcgcgtcct cgaggaggcg ctgcttcacc gcggcctgcg
     1141 catccgcatc caaatgttgc atggagcagc caccacacac gccgtaatgc gggcagcgcg
     1201 gcgccacgcg ctgcgcgctc gggcgcagca cgcgcaccac ctcaccctgc tcgtaactcg
     1261 gtttggcacg ccgcacgacg tattcgacgc gctcgcccgg cagggcgccc tcgacgaata
     1321 ccgccttgcc ctcgacgcgg gtcacgccac ggccttcgtg gtccagggaa tcgatcttgg
     1381 ctgtcggcat ggtcgtgcga gagggttcgg gaagcgcgcg attctaccgc gccacgcctg
     1441 cgcggatgtc cgcttcaggc aatgtctcct tacctttggg ccccacttgc gcgccgccac
     1501 ctcatccgga cgacatgatc ccccatgcta gaatctttgt gcattgcggc atccagcgcg
     1561 gaagacgggg tctcccggcg tccgccgaaa caagccgtac gcaccccgac ccccagaaca
     1621 aggaagacag accatgaagc attccatcct cgccgccgcc ctcgtcgccc tcgccgtgtc
     1681 tgcctgcggc aagaaggaag agccggtcgc ggttcagccg gcaccctcgg cccaggaaca
     1741 gatgaaggaa tcggccgccc aggcggtgga agcggccaag gagagcgcga aggccgccgg
     1801 cgacgctgcc gcggccggcg cgcaggccgt caagcaagag gccgccgcgg cggtcgaggc
     1861 gaccaaggaa gccgccgcta ctgcggccgc caaggcggac gctgctgccg acgcggctgc
     1921 gaagaaggcc gacgccgcgg tcgaggcggt caaggacgcc gccgccgacg ccaaagccga
     1981 caccgtcgat gccgcggccc aagccaaggc ggccgccgaa gctgccgtcg gcgccgccaa
     2041 ggaatcggct gcgaagtaag aagcccccgc tccacggctc gaatgaaaaa agccggctgc
     2101 atgccggctt ttttcatgct gtcgccacct cggggaagac cgcctccgcc cccatgagac
     2161 gcgacctgcg gcggtgccat cccctcttga cgctcagcgc agctgttccc gcagcaggcc
     2221 cagcatgcgg cgcgcggtgt cggagttgtc gagcccgccc tcgcgggtga gcacgctgac
     2281 tcgcgaggcc tcgccctgcc cctgcacgcg cagacggtat tcgctgccac cggcggccgg
     2341 tttggcatcg ctgcgccaga aggccagccg cgacaggaag cctttctcgg tgctgcggtt
     2401 gtccgcgtcg ggatcgacgt accgcacgaa gtacaagccc tgcgcacggt cgcggtcctc
     2461 gacggtgaag ccgatgcggt cgagcgcgag gccgacgcga cgccaggcac gatcgaatgc
     2521 ctcgtccatc accagcatca cctggccgtc gggcgcggag gcgatgcgcg cccgctccgc
     2581 ctgctgcggc gtggcgatcg cgacctcggc acgcgcctcc tcggcaccga ggcggaccat
     2641 caggcgacgc agcatctcgg cctcgagctc ggggtcggcg ggacgcggct gccagatcgt
     2701 cgagtccttg gcctcggtgg ggtagatttc catcatgccg cggtgcgaga tgtagacctc
     2761 caccgtgccc gtttccttgc cctgttcgag gcgggtgcgg aacttgtcgc gctcgggggt
     2821 cgagaacagg ccgtcgagca ccttgccgat gctggagcgg aggaaatcct gcgggatctt
     2881 ggcgcgatcc tcggcccagt cggtctccat gacgccgatt tccgggctgt cgatgttgag
     2941 gatgaagccg agctcgagcc agaaatcctt gatctgcggc cacagttcct cgggggtgcc
     3001 gcccaccacc agccagcgct ggctgcccgc acgctcgatg cgcatggtgt cggatttggc
     3061 gagcaccagg ggaccgctgg tggcctgcgc cggctggccg gcgcgatcgg ccgtgtaggc
     3121 ggagtaggtg gcgacgccgc gcggggcgac atcgggcacg gcgaagcggt cgtcgcgctg
     3181 cggtgcggtg agatcgggcg ggatctcgag ggggcgttcg atctgtttcg cgctcttgta
     3241 gtcgatgcgc ttggattcga gcagcgatcc cgaacaaccg ccgagcgcga tcgagagcgc
     3301 gagcagggac agggaggtgc gcttggagcg gatcatggaa gtcctgggca gagagattcg
     3361 gatcaggcct ggatgccggc cttgcgcatc gccgcgcgca cgcgctcgtg atgggctgcg
     3421 gacagcgggg tgaggggcag gcggaggccg tcgccgatca gccccatgcg cgccaccgcc
     3481 cacttcaccg ggatcgggtt ggcctcgcag aacaggtcgc gatgcaggcc ggtgagcccg
     3541 gcattggttg cgcgcaaggt gcgcatgtcg cccgcggccg cggccgcgca cagcgcatgc
     3601 atcgcgcgcg gcgcgacgtt ggcggtcacg gagatgacgc catggccgcc gagcatcaga
     3661 aaggcggccg aagtcatgtc gtcgccgctg tacagcgcga actccgccgg cgcgcgctcg
     3721 acaaggtcgc aggcgcggtc gaggttgccg gtggcgtcct tgagaccgac gatgttgggc
     3781 acctcggcaa ggcgcagcac ggtgtcgttg gcgaggtcgg cgacggtacg gccgggaacg
     3841 ttgtagagga tcagcggcag ttcgaccgcc tcggcgatgg tacggaagtg gcggtagagg
     3901 ccttcctggg tgggccggtt gtagtaaggc accaccgaaa gatgcgcgtt cgcaccggcc
     3961 tgtgcggcga agcgcgcgag ttccaccgct tcggcggtgg agtttgcacc ggtgccggcg
     4021 atcaccggca cgcgccccgc gcagtgctcg acggccacgc ggatcagctc gcaatgttcg
     4081 tccacggtga ccgtgggcga ctcgccggtc gtgccgacga tcacgatgcc gtcggtacct
     4141 tcggcgatgt gccagtcgat gaggctgcgc aggcggggga agtccaggct gccgtcgtcg
     4201 tgcatgggcg tgacgatggc gacaagcgat ccggtaatca tggcgtccga cagtgaatta
     4261 aagctgggat tctaaccgat cgccgcgcac ggtcgagcct gcctctgtta caggacgcgg
     4321 ccggcgtgcc ccgtatgcgg gcgcgccggc cggcggcaac gcaggcggcc cggcgagcgg
     4381 gactcagaga tcggccagcg tcttgatgtg ggccactacc gagcgcgcca gcgaggacag
     4441 cgaatagccg ccttcgagta tcgagacgat gcgcttgtgg ccgcaggact cggcgactcc
     4501 cttgatctgc tgggtcgccc agatgtagtc ggcctcgagc agcccgagcg agcccatgtc
     4561 gtcctcgtag tgcgcgtcga atccggcgga gatgaagatc atctgcggac tatggctgcg
     4621 cagcgcgggc acgacgatgt cgccgaagac ctggcggaag gcatcgcccc gcgtacccgc
     4681 cggcagcggc acgttgcaca tgtgcgccgg ggggttctcg gtaccgcaat acgggtagaa
     4741 gggatgttgg aagatccccg ccatcatgac ccgcgggtcg ttgcggaaga tgtcctcggt
     4801 gccgttgccg tggtgcacgt cgaagtcgac cactgcgacg cgctccagcc cgtgggcttc
     4861 gagcgcatgg cgggcggcga tggcgacgtt gttcatgaag cagaacccca tcgccttggc
     4921 gcgttcggcg tggtggcccg gcggacgcac ggcacagaag gcgttctcga tctccccctt
     4981 catcacgagg tcggtggcgt agacccccgc accggcggaa cgcagcgccg ccttcatcgt
     5041 gcccggactc atcgcggtgt cgggatcgag gtggcggatg ccgtgctccg gcacgctctc
     5101 gatcaggttg tcgagatgct cggccggatg cacgcgcagg atgtgctcgc gctcggccaa
     5161 aggcgcatcg aaaaaggaca catacatatc gagccccgag gcgatcaggc gatcgttgat
     5221 cgccgacagc cggtcgggac actcgggatg catcgcaccc atgtcgtgca gccagcagtc
     5281 acggtgagta atgaatgctg tcgtggtcat gctcctccct ctcgcctcgc ggacgacgga
     5341 cacggggcac cagacacccc cttccgactc tggcggccgc cgaccgtgtt gcgtccgatc
     5401 gtcgggccgc ttgcctttgt cgtctattat caccgcttcc aggccaattt cacagtgcgc
     5461 acgatgaccc tgcaccatgc gaatcgtctg ctcgaagccg cctcgcgcat catcctcggc
     5521 aaggagcgcg agctgcgcct cgcgctcgcc tgcctgatcg cgcgcggcca cctgctgatc
     5581 gaggacgtgc ccggcgtcgg caagaccacg ctcgcacacg cgctcgcccg cctgatgggg
     5641 ctgcagttcc agcgcatcca gttcaccagc gacttgctgc ccgcggacat cctcggggtc
     5701 tccatcttcg accgcaacac cgccgccttc cgcttccacc ccgggccggt gttcgcccaa
     5761 ctcatcctcg ccgacgagat caaccgcgcc acgccgaaga cgcagagcgc gctgctggaa
     5821 gccatggagg agcgccaggt caccgccgac ggccagacct gcgcgttgcc cgaacccttc
     5881 ttcgtgattg ccacgcagaa ccccgcccac cagatcggca cctttccgct gcccgagagc
     5941 cagctggacc gcttcctgct gcgcatccgc ctcggctacc cggacccgca ggccgagcgc
     6001 gcgctgctga tgggcgagga ccggcgcggg ctgctcgagc gccagccggc cgtcatcggc
     6061 ccgggcgaac tgctcgacat gcagctggcg gcgcaaggcc tgcatgtcgc cgaggccctc
     6121 gtcgcctacg tccagaccct gctcgccgcc acccgcaaca gtgcggaact cgccggcgga
     6181 ctgagtccgc gtgccggtct cgggctgctc gccgtggcgc gtgcatgggc actgctcgac
     6241 ggccgcgacc acgtgctgcc ggaggacgtg cagaccctct tcccgcacgt cgccgcgcac
     6301 cgcctgcacc tcgccggcga cggccgcccg gcgccggcgg cgacgctggt gcggctgctg
     6361 cagaccgtcg ccgtgggctg atggcgcgca ccgccgcatc gctcgccacg cggctgcgcc
     6421 acgccggcga gcgctggctg ttccgcatcg gcgccccgga gcctgctccg atccgcctcg
     6481 gccagcgccg catctacgtg ctgccgagcg cggcaggtgt cggcttcgcc ctcgccctgc
     6541 tggtgatgct gctcgcggcg atcaactaca acctgagcct cggctacgcg ctcgtgttca
     6601 ccctcggcgg cagcgccgcg gcgagcatac tgcacgcctt ccgcaacctg tacggtctgt
     6661 cgatccgccc ggggcgctgc gcgccggtgt tcgcgggcga gaccgccgtg ttctccctcc
     6721 tcatcgacaa cccggccagc cgacggcgcc ccgccttacg cctgggcgcg cagggacaat
     6781 ggagcgcgtt cgatctcccc gccgcggaag agaccgccgt cgccctggcc tgccccgcgc
     6841 tccggcgcgg ccggctcgcg ctcgggcgca gcgtgctcga gacgcgctgg ccgctcggcc
     6901 tcatccgggc gtggagcgtg ttcgtcccca aggccgactg cctggtgctg cccacgccgg
     6961 aagccgatcc gccgcccctg ccccccgcag gtggcgacgc gaccggcgcc agccgcgacg
     7021 cgcacacggg tgacgaggac ttcgccggct tgcgcgcgca ccgtaccgcg gactcgcccc
     7081 gccacgtcgc atggaaggcc gtggcgcgcg gcggcccgat gctgaccaag gagttcgccg
     7141 caggagaggg gcgcgagctg ctgctcgact gggaccgcct gcccgccgga ctcgacgccg
     7201 agcgccgact gtcgcggctc accgcctggg tactcgccgc cgagcgtgaa ggacgccgct
     7261 acgcgctcgc cctgcccggc atccgcatcg cggcagcaac tggccgcgcc catcgcgagc
     7321 gctgcctgcg cctgctcgcg ctccatggcc ttcccgatcc gacagcggag gatggcgcat
     7381 gagcgtggct cgcgcacggc ggctgatcgg cctccgccgc cgcggcggcc cgctgggtgg
     7441 cacacgcacc ggccccctgg cgcctgcgct gcggcgcgac cagggcgcct ggctgctcac
     7501 cgccgccgcg atggcgatcg cccctcatgc gggctggctg ccggggtgga tcggggcgct
     7561 cggcctcggc ctgctcgggt ggcgtgcgct gctgctgtgg cgcggcagcc ggccgccgcc
     7621 gggcaccctg ctgttcgcgc tcgcgctcgc agtcgccgca ggggtacgtc tggagttcgg
     7681 ccacttcttc ggccgcgagc cgggcgtcgc ggtgctggtc ctgctgctcg gcctcaagct
     7741 gctcgagacg cgcgcggcgc gcgacatccg cgccggggtg ctgctgtgcc tgttcctgca
     7801 gttggcgctc ttcttcgagg atcagtccct gcccgtggcc gcgctcgtgc tcgccggcac
     7861 cctgcccgca ctcggcgccc tggtggcgct cgccgacccg gacgggggcg agcgcgaacg
     7921 cctgctcacc gcggccaccc tgctcgctca gggcctgccc ttcatgctcg cactgttcgt
     7981 gctcttcccg cgcatccagg gcccgctgtg gggcctgccg gcggatgcgt tctcggcgcg
     8041 caccgggctg tcggacacga tgcgcccggg ctcgatcagc gcactcggcg aatccgacga
     8101 gatcgccctg cgcgccgcct tctccggccc gccgcctcca ccggcgcagc gctactggcg
     8161 cggcccggtg ctcacccgct tcgatggccg cgaatggcac gccgagccgg ctgccgaatc
     8221 cgcctcgccc agctacacgc cgcagggcga gcgcatcgac tacctgctta ccatcgagcc
     8281 gcaccagcgg cgctggctga tcgcgctcga acatccgggg ccggcgcggc cgccgatccg
     8341 ctataccggc gacctgcgcg cgctcgccgc cgaacccctg cgggcacggg cgcgcttcgc
     8401 cctcggcgcg tatccgcaca cgccagtcgg cgtggccgaa tcgcccgcgg tgcttgcctc
     8461 ggccaccacc ctgcccgccg acagcaaccc gcgcagccgc cgcctcgccg ccgaactcgc
     8521 cgctggcgcg cgcgaccacg ccgagatcct cgagcgcgtg ctcgcccgac tgcgcgccct
     8581 gcgcctggcc tacacgctgc gcccgccgct gctcggccgc cacgcggcag acgagttcct
     8641 cttcgacgcc cggcgcggct tctgcgagca cttcgcctcc gccttcgccg tgctgatgcg
     8701 cgccgcaggc gtgccgaccc ggatcgtgac cggctatcag ggaggcgaga tcaacccgat
     8761 cgacggtcag ctggtggtgc gccagtccga cgcccacgcc tggaccgagg tctggctgca
     8821 gggacgcggc tggctgcggg tggacccgac cgcgctcgcc gcccccgagc gcatcgacgg
     8881 cgggctggcc gcggcgctgg ccgacgcggg cgagttgccg ctgatgttgc gcgccgacat
     8941 ggcctggctg cgcggcctgc gccaccgctg ggaagctgtc gcgaacctgt ggaaccagca
     9001 tgtccttggc tacaatcccg agcgccagcg cgaactcctc gcgcgcatcg gcctcggcgc
     9061 ggacagactg gcgcagaccc tgggcgtgct cgccggcgca gcggtactgc tgttcgccgc
     9121 cctctatgcc tggagcctgc gcgccacgcg cgtgcgcgac ccgctcgcgc gcacctggga
     9181 gcgcttctgc gcgaagatgg ccgccgctgg cgcggcccgc ccagcctggc aggggccgca
     9241 ggactatgcc gaagaactgg ccacgcactt cccggcgcat gccatggaac tacgcggcat
     9301 ctgcatgctc tacgcccgcc tgcgctatgg accgcccgcc ccggagcagc aactccggat
     9361 tctgcacaac cgcatcgcct cactgcgcct gaaatgatcc cgaccccgtc cctccccgcc
     9421 cgcccttcct cgcccacgca gcggccgccg taccggcagc gcaagctcgg cgggatcctc
     9481 gccgccttcg cgatcgcgct gtgcacgccc gcgctcgcgc atgcgtccta cgccgagcgc
     9541 gaggacgcgc gcgccttcgc cgcggaaatc gccagccgcc atgcactcga cgccggcgag
     9601 atcgagcgcg cgctcgccgg cgcccgccac gatccggagg tgatccgcct gatcacgccc
     9661 ccctcgcgca agggcgtgcg ttcctggcgg tcctatcgcg cgcgtttcct cgaccgcacc
     9721 cgcatcgacg gcgggatgcg tttctggcat gagcacgccg ataccctgga acgcgccgag
     9781 cgcgagaccg gcgtccccgc cgaggtcatc gtcgcgatca tcggggtgga gacgatctac
     9841 gggcgcaaca ccggcaactt cgagaccctc tccacgctcg ccacgctggc cttcgactac
     9901 ccgccgcgcg ccgaactctt ccgccgcgaa ctcgaggccc tgttcctgct cgcgcgcgaa
     9961 cagggacgcg acccggcgag ctattacggc tccttcgccg gcgcgctcgg ctacccgcaa
    10021 ttcctgccga gcagcatgcg cgcctatgcg gtggacttcg accgcaacgg cgcgatcgac
    10081 ttcgaaggtg atccggtgga cgcgatcggc agcgtggcca actacctgcg cgcgcatggc
    10141 tggcaggcag gcgcaccggt cgcagagcgc gctcgcctgt cgcccgcgac cgatgctgcc
    10201 ggcctggtgg cggccggcat cgagccgacg ctcgcgcccg cgctgctcgc cgacaacggc
    10261 atcaccaccg ccgacggccg ccctgtcgcc gccgcggcga ccctggtgga cctggagacg
    10321 ccgggcgccg acaccgagta ctggctcggc taccggaact tctacgtcat cacgcgctac
    10381 aatcgaagca gtttctacgc gatgtcggtg ttcgagctcg ccgaggcgct gcgcctgggc
    10441 cggctggtgg aggagatccg cgccgaacgc cgctgaagga tccgcccatg cccgcaagcg
    10501 ccccgccgtc gtcctccgac ccctcctccc tccgtccggc gccgctcggc ctcgccgacc
    10561 tcggcggcgc gctgcgtttc ggcatcacgc agttccgggc cattccgctc ctgagcatgg
    10621 ccttcgcact actcttcgtg gtgatcggag ccctgctctt cggcattctc gagttcggcg
    10681 cgatcgcgcc gatgagcctg tcgctcgccg ggggcttcat gctcgtgggt ccggtactgc
    10741 tggccggctt cttcggtgtc tccgacaagc tgcgcaaggg ccgccagccg agcttcggcg
    10801 acatctggag cggttttcgg cagatgccgc gcggcggctg ggtggtgtcc ttcgtttgcg
    10861 ggctgctgtt cctgatctgg atcaccgacg ccggcatcct ctacggcttc atggtgggcc
    10921 gcgagccgat cggcttcgtg cgcctgctgc gaatcgagca ggtcgtgatg gactacgccg
    10981 gttacagcgc gatcatgggt ggagtgctgg ccttcatcct gttcgccgtg tcggcgttct
    11041 cgatcccgct gatctacgat ggccgcgcga cgctggtgtc gggcgtggtg gcgagcgtgc
    11101 gtgcggtgtt cggtcgcttc ggcgtgatga tgtgctgggc cttcctgctc gccgcggtga
    11161 tcatgggcag cgtcatggcc ctgccgctgc tgctggtcag cctgccggtg atggcctacg
    11221 cgagccggga actctacttc cgcacctttc cccctgccgc gcccgcgaca tgacgggact
    11281 cctgcgcgca cccgccgttg tgcttgccta gagcagcgcg tcgcgcgaga ttccgcgccg
    11341 gcgcaggatc cggcgcagct ggcccagcgc ctcgagctgg atctggcgca cgcgctcgcg
    11401 ggtgagttcg aggcgttcgg cgagttcttc gagcgtgagc aactcgcact cgtcgatgcc
    11461 atagcggtga cgaatcacca tgcgctgctt gtcgttgagg atcgcgagcc attcgcgcac
    11521 gagttgctcg acttccgagc actgtatgcg aaggtcggcg gagatctgct gctcgtccgc
    11581 gagcgactcg ccgatcgaca aggaggggtc gatatcgagc ggcgcgtcga gcgaggcggt
    11641 gtgctcgccg agcgcgagga tgctgcgcac ctcctcgatc gtcttgccta gccgattggc
    11701 gatctgctcg agcgaggact cgccgttgca atcggcctcg atgtggcgct gcgcgcgcag
    11761 gacctgattg agttccttca ccacgtggac gggcaggcgg atcgtgcgcg actggttcat
    11821 gatcgcacgc tcgatgttct gccggatcca ccacgtcgcg taagtcgaga agcggaagcc
    11881 gcgctcgggg tcgaacttct cgagcgcgtg catgaggccg aggttgccct cctcgaccag
    11941 atcgagcaag ggaatcccgc gattgaggta gtgcttggcg atattgacga ccaggcgcag
    12001 gttgcgctcg atcatcgtct gccgggcatc gaagtcgccc atccgcacgc ggcgggcgat
    12061 caccagctcc tcctcggcgg tcagcagcgg attggcaccg atctcgttga ggtagagctg
    12121 ggtgacgtcg ctgaagaact cgttctcgac aaccggcggc gcctggaacg agaacacctc
    12181 cacctcaggg ggaagatccg gttcctgctg actttccagt tcatcaagat tgaccggttc
    12241 ttccatcctc gccctcctca gcgcgcaggc agatacccgg ccggatcgac cgccttgccc
    12301 tgcttgcgga tctcgaaatg aagcttgggc cgatcggctt ccgaactgcc gagctcggcg
    12361 atcttctggc cctgtgcgac ctggtcgtct tccttgacca ggagcttgtc gttgtgagca
    12421 tacacggagt tgtactcctg attatgcttg atcacgatca atttgccata accccgcagg
    12481 ccgctgcctg cgtagaccac cttgccggcc gctgccgcat acaccggctc gccgaccgcg
    12541 ccaccgatgt cgagcccctt gttggaagcc tcgttgaagc cggcgatcac gctgccggag
    12601 gccggccagc gccattggcc gctcatcgtc gaaggcggcg cctccgccgc ggcgggcggc
    12661 ggactctcga ccgcagcggg ctcggtcacc ggcgcacggc cgccgaccgg ttcccgcacg
    12721 gtcgggaccg tagcggagcc ggtcgaaggg atcggcgtcg cgatggcccc cccagccccc
    12781 gcgatcgagc ccgtcgaggc cgacggcgac acgcgcaggc tctggccgac gtggatctgg
    12841 tcggggtcgg tgatccggtt ccacgacacc agatcgggca gggtgacgcc atattggcgt
    12901 gcgatgccga gcagggtgtc gcccggacgg acggtgtgat aacccgaggc cggcgcgacc
    12961 gcagcggccg gtgcgggcga aaccccgcta ccgtcgcgca ccggcgccgt cccctgcgag
    13021 gcgcagccgg caagcagcgc ggcggcgacg agggcgatga gatgagaagt gttcttgtgc
    13081 atccgaaaat accgtgattc agtcagtacc cgcgatcagg ggaacgaagc gcaccgcctc
    13141 gcaccaggtc tcgcggaagg tattgccctg gcgctcgatc agcaacaggc gctgctccgc
    13201 tccaccgacc ggaatgatca gccgcccgcc cgaggcgagc tgctccttca atgccggcgg
    13261 cagcccgacc gccgccgcgg cgacgacgat gctgtcgaag ggagcggcct cggggagacc
    13321 aagactaccg tcggcaagtt tcagccgcac gttgggcagg cgcaaggggc gcaggttcgc
    13381 gcgcgcgcga tcgagcaggc cgcggatgcg ctctacggca tagacctcgg tggcgagcag
    13441 cgacagcacc gccgcctgat aaccgcagcc cgcgccgacc tcgagcgtgc gcccgagctc
    13501 gcgcccgttg cgcagcagtt cgagcatgcg cgccaccacg aagggctgcg agatggtctg
    13561 ctggaagccg atcggcagcg gcgtgtcgtc gtaggcgctg aatgccagcc cctcgtccac
    13621 gaaggcgtgg cgaggcaccg cctgcatcgc cgcgagcacg cgctcgtcgc ggatgccttg
    13681 ggcgcgcagc cgctcgacca tgcgcgcgcg tgcgcgcgag gcctgccccg ggtcggatgc
    13741 gcgcacgctc accgctgcaa ccagtcgaag accggagcga tcaggccggt atgggtgagg
    13801 tcgatctgca gcggcgtgac cgacacgaag ccgttggcca ccgcatggaa gtcggtgcct
    13861 tctcccgcgt cggcggcacc gcccgcggca ccgacccaat agaccgtctc gccgcgcggc
    13921 gtctcgctgc gcaccaccgg ctcggccttg tggcgcttgc ccaggcgggt gacgcggatg
    13981 ccttgcaggc gctcgtaggc gacatcgggc acgttgacgt tgagcagcac cggacgcgga
    14041 aaggggttgc gcatgaagcg ctcggcgagg tcgcgagcga cgcgtgcggc ggcggtgaaa
    14101 tcggtggccg acttgctcac cagtgagacc gcgatcgagg gcacgccaag cagaaagccc
    14161 tcggtggcgg ccgcgaccgt gcccgaatac accgtgtcgt cccccatgtt ggcgccgtga
    14221 ttgacaccgg agacaaccat gtcgggaagg tggtcgagca tcccggtgac cgcgagatgg
    14281 acgcagtcgg tgggggtgcc attgacgtga tagaagccgt tggaggtctg gcgaagcgag
    14341 agcggacggt cgagcgtcag cgagttgctc gcgccgctgc ggtcgcgctc gggagcgacc
    14401 acggtcacct ggccgatcgt gccgagcgcc tcggcgagcg ccgcgatgcc gggggcgaag
    14461 taaccgtcgt cgttgctgac caggatgcgc atcttgattc cgtgcgggtg gctgcgaggg
    14521 attgcaaggg cctgcaaggg gcgccgcgaa ccggcgggag cggagatatt agcatggcgt
    14581 accaggccgc acgcctggcg aacggcacaa acaaaaaaac cggcctgggc cggttagttt
    14641 gattggtcgg ggcggcggga ttcgaactcg cgaccccttg caccccatgc aagtgcgcta
    14701 ccaggctgcg ctacgccccg acacaagccg ccattatagc agcggttttc agaaatacca
    14761 gtacttttgc tgtgcgatga tcagcctgcg aatcgtgccg cggatcaggc gcgcagttcc
    14821 tgcagcgtgg ccgcaatctc ggcgcgcagt tgccgcagcg tggacctgca ttgctcgagt
    14881 tccacgccgt tctcctgctc ctggatcgcc gactccccaa ggcggttgcg cgcaccggag
    14941 atggtgaacc cctcctgata cagcagctgg cggatgcgcc ggatcagcat gacctcgtgg
    15001 tgctggtaat agcgccggtt gccaccgcgc ttgaccggtt tcagctgggt gaactcctgc
    15061 tcccagtagc gcagcacgtg cggcttgacg cagcacagct cgctcacctc gccgatcgtg
    15121 aaatagcgct tcgcaggaat cggcggcagt tgctcgccac cggtttcagg gctgcttgct
    15181 tgcatcgctg agttgctcca ccgcggcctt cagtttctgg ctggcatgga aggtgaccac
    15241 gcgccgggcg gtgatcggga tttcctcgcc ggtcttgggg ttgcgacccg ggcgctgcgg
    15301 cttgtcgcgc aactggaagt tgccgaagcc ggagagcttg acgctgtcgc cgcgctccag
    15361 cgccgtccgg atctcctcga agaagccctc caccatgtcc ttggcttcac gcttgttgag
    15421 cccgacctgc tcgaagagga ggtcggcgag ctcggccttg gtgagggtca tgtcagcctc
    15481 ccgcgcctcg caggcgcgcg ccgagcaccg tttcggcatg cgtcacgagc agcgcgaccg
    15541 ccgcgtcgac ttcggcatct tcgagggtgc gacgagtatc ttgcatcgat accttgaagg
    15601 caaggctctt cttgtcggag tcgatgccct tgccctggta aaggtcgaac agcgcgatct
    15661 cgcgtacgag cgcgggcgcg gcctcgcgca gcacctcgag cacgcgctcg accggaacct
    15721 cgttgcccac caccagcgca agatcgcggc gcaccgcctg catgcgcgag atctcggcat
    15781 aggccggcac gcgggcggcg agcgcagcat cgagcgcaac ctcgaacacc accggcgcgg
    15841 tgccgagctc gtagcgctgc acccacaccg gatgcagttc accgagcacg ccgatcatgt
    15901 gcccgtccag gcgcacggcg gccgcgcgcc ccggatgcag ggcgggatgc gagagcgggg
    15961 cgaactgcag ctcgcgcggc gcgaacagcg cctcgagatc gcccttcacg tcgtaaaagt
    16021 cgacgctgcg cgcgcgctcg ccccactgct cgggcaccac ctggccggcc gccagcgcgg
    16081 cgatgttcat cacctgatgg aagccgcgca ccggctcgcc atcggccttg cgctcgaaca
    16141 cgcgtgccac ctcgaaaacg cgcacgcggt cgatctggcg cttgcgattg gcggcgagcg
    16201 tgccgaccag gcccggaatc aggctggagc gcatcacgct gaggtggctg gcgatcgggt
    16261 tggccaggcg gatcgggttc tcgttggcgc agaagtcgcg ctcccaggcc tcctcgacga
    16321 aggcgaagtt gaccacctcc tggtagccgc gcgcggcgag caggtgacgc agcgcccaca
    16381 ccgggcgtcc gttctcgctg ctcggcagca tcatcaggct gccctgcggg gcgatcgccg
    16441 ggatgttgtc gtagccgtgc aggcgggcga tctcctcgat caggtcttcc tcgatctcga
    16501 tgtcgaagcg ccacgacggc ggcgtgacga ggatgtcgtc acctgtgcgc tcgacctgca
    16561 ggtggacgcg acgcagcagc gcgagcatgt ccgcgtcggc aagcacgatg ccgagcacgc
    16621 ggcgcgcacg cgccggacgc aaacgcaccg gcgcgcgcgc gggcagcgcg tcggtggcga
    16681 cggcctcgac caccggaccg gcctcgccgc cgcacagctc gatcaccaga cgggtggcac
    16741 gctcgagggc gctgcgcgca agctccggat cgacaccacg ctcgaagcgg tgcgaggcat
    16801 ccgagccgaa accgtaggcg cgcgcgcggc ccgcgatcgc atcgggcgcg aaaaaggcgc
    16861 tctcgaggaa gacttcggtg gtgtcgaggg tgatcccgct gtcctcgccg cccatgatgc
    16921 ccgcgagcgc gagcgcgcgc gcctcgtcgg cgatcagcaa ggtgtccgca gccggggtca
    16981 ccgtctgctc gttgagcagc agcacctgct cgcccgcacg cggcaggcgc acatggatgg
    17041 cgccctgcag cttggcgttg tcgaaggcat gcaggggctg gccgagctcg agcatgacgt
    17101 agttggtgat gtcgaccagc gcgctgatcg agcggatgcc gctgcgcacc aggcggcgca
    17161 ccatccagtc gggcgtcgcg gcgcgcgcgt ccacgccgcg caggatgcgg ccgcagtaac
    17221 gcggacaggc ttgcggcgca tcgagcacga tgtcgcggcg ggcctcgatc gcgggcgcga
    17281 ccgggtcgat ggcgagcgga cggaactcgc cgccggtgat ggccgcgacc tcgcgcgcca
    17341 cgccggtcag gctcaggcag tcggcgcggt tgggggtcag cttgatggtg aacaaggtgt
    17401 cgtccaggcc gaggtaggcg cggacgtcgg tgcccaccgg ggcatcctcg ggcagcgcgt
    17461 agaggccgga gtgatcctcg gagaacccga gctcgcgcgc agagcacagc atgccgaagg
    17521 actcgacgcc gcgcagcttg gccttcttga tctcgaactc gccgggcagg accgcaccga
    17581 ccaccgcgca tggcaccttc atgccggcgg cagcgttggg cgcgccgcag acgatctgca
    17641 gcaattcgcc ggtgccggcg tcgaccttgc acagcttgag cttgtcggcg ttggggtgct
    17701 tctcggcctc gacgatcttc gccaccacga caccgcggaa ggcgggcgcg gcgggttcgg
    17761 cctcctcgac ctcgaggccg gccatggtca gcagatggcc gagtccggcg ctgtccagtt
    17821 gcggatcgac gaaggtccgc agccactgtt ctgagaattg catgattcga ttacccggat
    17881 tgcgtggtag ggcgcagctt gtgcgaagcc gctcagcgga actggccgag gaagcgcagg
    17941 tcgccctcga agaacaggcg caggtcgttc acgccgtagc gcagcatggt gaggcggtcc
    18001 ggccccatgc cgaaggcgaa gccggtgtag cgctcggggt cgatgccgcc gaagcgcagc
    18061 acgttggggt gcaccacgcc gcagccggcg atctccagcc agcgcccctc gagcgcgccg
    18121 ctcatgaagg cgacgtcgat ctcggccgaa ggctcggtga aggggaagaa ggacgggcgg
    18181 aagcgcacct gcaggtcgtc ggtctcgaag aagcggcgca ggaagtcggc gatcacgccc
    18241 ttgaggtcgg cgaagctcac cgtctcgccc acccacaggc cctcgacctg gtggaacatc
    18301 ggcgagtggg tggcatcgga gtccacgcgg aagacacggc cgggcgcgat gatgcgcagg
    18361 tcgggcatgg catcggcgtc acggtggcgc gcgacgtggg cctgcatggc gcggatctgc
    18421 accgggctgg tgtgggtgcg cagcagcacg tcgtcgtggc cttcgagata gaaggtgtcg
    18481 tgcatcgagc gcgccgggtg gttctcgggc gtgttgagcg cggtgaagtt gtgccagtcg
    18541 gtctcgatct cgggaccgtc ggcgaccacg aagccgatcg agccgaacag gcgctcgacg
    18601 cgctcgaggg tgcggctcac cggatgcagt ccgccctgca cgccaccgcg cccgggcagg
    18661 gtgacgtcga gcgcctcggc ggcgagctgg gcgagcagcg cggcttcgcg cagcgcgttg
    18721 cggcggcctt cgagcgcggc ctcgatcgcg gtcttggcgg cgttgatcgc ggcgcccgcc
    18781 tcgcggcgcg cctcgggctc gagcttgccg agcgccttca ggcgctcggt gagcgcgccg
    18841 gacttgccga ggtagcgcgc cttggcctgt tcgagctgcg cgccgtcgac cgcggcggcg
    18901 aattcggcgg cggcctgttg taccagttga tcgagctgtt ccatgtccct gccctggcgg
    18961 aatgcagtgt tgcgggatga gcgctgcgga gcgcaccgct catcgggaat tcgggggagt
    19021 ccggtcgcgg cgcccgccgg gcgccgggaa tccagaaaaa aaaaggaggc caggcctcct
    19081 tttttttggc tgcgcgcgat tacgcagcga gtttggcccg ggcctgctcg gcaatcgcgg
    19141 cgaacgccgg cttgtcgaac acggccagat cggccagcac cttgcggtcg atctcgatcg
    19201 cggccttctt gagaccgttc atgaacacgc tgtaggtcag gcccagctcg cgcgcggcgg
    19261 cgttgatacg cgcgatccac agggtgcgga actgacgctt gcgctgacga cggtcgcggt
    19321 aggcgtactg acccgccttc atcaccgcct gcttggcgat gcgatagacg ttcttgcgac
    19381 ggccacggta acccttggcc tgggagagga ctttcttgtg acgggcgcgg gcggttacac
    19441 cacgtttgac tcggggcatt gcggtctcct atcaggcgta cggaagcatg gcgcggatca
    19501 gcttttcatc tgccgcatgc acttcggtca tgccgcgcag gtgacgcttc accttcgtcg
    19561 tcttcttggt caggatgtgg cgcttgaacg cctgggaccg cttgatcccg ccgctggagc
    19621 ggaccttgaa gcgcttcttc gctccgctct tggtcttcat cttgggcatt gctgagactc
    19681 catgtttttg gatgacacca ggtagcaccg aaaagcccgg cgcgttttgt tacccggcac
    19741 cacttgtttt ccgccggagg gaaattcctt cccgcggtct cgcccctcgc gaggcgattt
    19801 cttcgggaag aagctcagtg cttcttcttc ggcgcgatga tcatgatcat ctggcgccct
    19861 tccatcttgg gcatctgctc gacctggccg agttcctcga ggtccgcctt cacgcgctcg
    19921 agctggcgca ggccgaactc ctggtgcgcc atctcgcggc cgcggaagcg caaggtcacc
    19981 ttgcacttgt cgccttcttc caggaagcgc ttgaggttgc gcagcttgat ctggtagtcg
    20041 ttctcgtcgg tgccggggcg gagcttgacc tccttgacct gcacctgctt ctgcttgagt
    20101 cgggcttcgt gggccttctt ctgttcctga tacttgaact tgccgaaatc catcagcttg
    20161 cacacgggcg gcacggccat cggtgcgatt tcgaccagat ccagcccggc ctcttcggcc
    20221 atgctgaggg atgcgttcag ggagacgata ccgagctgct cgccctcctc gccgaccagc
    20281 cggacctcag gcgcagtgat ctcccgattt acgcgctgct ttttttcttg tgcgatggtt
    20341 caactctcca cgaaaaccaa tatcagaccg atccggcttt cgcttcaact tcgcgaagcc
    20401 agcgttcgat cagcgaatcg agggacattt ggccaagatc ctggccaccc cgggcacgta
    20461 cggccaccag gcctgccgcc ttctccttct cgccgatgac gatctggtac ggcagcttct
    20521 gcacgctatg ttcgcgaatt ttatagttaa tcttctcgtt gcgcaaatcc gcctcgacgc
    20581 ggaagccggc cttcttcagc ctcgccaccg cctcttcggc atattcggcc tggccctcgg
    20641 agatgttcat caccaccgcc tgcaccgggg cgagccacag cggcatggcg ccggcgtgat
    20701 gctcgatcag gatgccgatg aagcgctcca gcgaaccgag gatggcacgg tgcagcatca
    20761 ccgggatctt cttggtgttg tcctcgtcga catactcggc accga
//
LOCUS       DAOQBS010000047        20730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1757_length_20730_cov_16.099492, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000047 DAOQBS010000000
VERSION     DAOQBS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 20730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20730
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1757_length_20730_cov_16.099492"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PKN25_07245"
     CDS             complement(<1..755)
                     /locus_tag="PKN25_07245"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNV90729.1"
                     /translation="MNANEKFIAAKAHVDEAAIAPLPNSRKIHVEGSRPDIRVPMREI
                     TQADTPASFGAEQNPPIFVYDCSGPYSDPAAKIDIRSGLPALRQQWIEERGDTELLPD
                     LSSEFGRQRAADKTLDELRFPGLHRKPRRAKAGANVTQMHYARRGIITPEMEFVAIRE
                     NLNREQYIASLRASGGLKGQKMADMMLRQHPGQNFGASLPATITPEFVRDEIARGRAI
                     IPNNINHPESEPMIIGRNFLTKINANIGNSAVTS"
     gene            complement(772..1665)
                     /gene="thiD"
                     /locus_tag="PKN25_07250"
     CDS             complement(772..1665)
                     /gene="thiD"
                     /locus_tag="PKN25_07250"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNV90730.1"
                     /translation="MSSSASSAAPGSAPSASSGTASRIPNVVSIAGVDPSGGAGVFAD
                     LKTFSALGAYGCGVVAALTAQNTRTVTGVHVPPTDFLRLQIDTLFEDVAIHATKIGML
                     GSAEVTTTVAERLAHWQAARVVLDPVMVAKSGDSLLAKSAISALREALFPQAFLITPN
                     LPEAGVLLERRAPDTVKEMYRAAERLRELLPLSSERWVLLKGGHLPGNDLVDLLFDGD
                     RMVELPAQRIETKNTHGTGCTLSSAIAALLPQAEGQFRPVETAVRHARQWLLGAIARS
                     GELAVGSGHGPVHHFHALWRK"
     regulatory      complement(1659..1754)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1852..3684
                     /locus_tag="PKN25_07255"
     CDS             1852..3684
                     /locus_tag="PKN25_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNV90731.1"
                     /translation="MKNPRPLLLAVLLAHVAGAVCAADWQLVLSDRDRRIEIDRASVL
                     RSDAGTKISWGRVVLTSEEARRSGYAMIKALNRYDCRNRSFATVKRVYVDADDNIVRE
                     EPVAAEAPIAVARNTVDERMWREVCDPPSAVELQKMAAEAARIASAQPAAVAAPGGAA
                     APAAAAVSTPPAAAAATAVQSPAAKPAESARAQSGSVRQPSAESVSAEVVRTGAGGEQ
                     PAAAPPAFETHVEIPAKFLPGGQGAAAPESSRKSILPPLPKLSATAEPEAKTAPAPKP
                     PVREQPPAQGRAAPAPQAAVPARQAAPAPERPVAPERAGAREKASPARAASAVPARPT
                     PPRPRPAGPERVAAAQPASPDATPAPPERAASAREEALRAAGIELTRLGPGVPEWSYE
                     GERGPQHWGRMRPEWRLCEEGTRQSPIDLRDGIAVDLAPVRFDYRRTGFRIRDTGNTL
                     QVEVGEGMGITVRGVRYALERLTLHRPSQDRVGGMAHDMAIYLQHRADDGRMAIVSLL
                     LSAGGDASPALQTLWNNLPLDRGREFVPDAVLDLPALVPADPGHYLYTGSLPMPPCTE
                     DVLWVVMKQPVTISADQLDVFARLYPRNGRPIQPTNGRPLLESR"
     gene            3893..5005
                     /locus_tag="PKN25_07260"
     CDS             3893..5005
                     /locus_tag="PKN25_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV90732.1"
                     /translation="MRFRTLPTLLAGLFVLAACSAPAPHTPPPPAVLVRSFAAANAPP
                     SVQVYAGEVRARVESDLGFRIGGKLVERLVDVGAEVAAGTPLALLDAQDVRLALASAQ
                     AAAAAAESDLALARTEFARARELQARNFVSSSLLDARRTALQAAEARLRQARAQAEVA
                     ANQAGYARLEAPAAGVVTAVLAEPGQVVGAGQAVLRLARPDEREVLIHVPEGRTGRIA
                     PGSAAQVRPWAAPERAYAGRVREVAPAADAATRTHAVRVAVPGADATLALGASASVAF
                     AAADGEAGGVLLPLPAVTRQPGQADAGTVWVVGEDDAVRPLTVELGAWREDGVVVRAG
                     LPAQARVVVAGVYKLIEGMRVRAVEEGAPVLLDVRR"
     gene            5002..8223
                     /locus_tag="PKN25_07265"
     CDS             5002..8223
                     /locus_tag="PKN25_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV90733.1"
                     /translation="MSMKGEGPIGSGRFNLSEWGLTHRSLVLYFLIVCTLSGMFAYTR
                     LGQSEDPPFTFKVMVVRAEWPGASAREVEQQVTDKIEKKLQEIAQADIIRSYSKPGEA
                     MVFFVAKDSTRARDIPDIWYQARKKIGDIAHTFPTGVRGPYFNDEFGDTYGNVFALLG
                     EGVSHAELERHAEAIRGELLRVKDVAKVSFFAEQPQRVFVEISNTRLATLGVRLDDIV
                     GALARQNAETGAGFFETGDERIWLRPSGQFEDLDAIRATVIRAGGRSLRLGDIAEVRR
                     GYADPPAERLRFMGQEALGIGVSMRSGGDIVALGRQLDAAVEKIVAQLPVGIRLERVA
                     DQPRAVTRAVDEFVKVLAEAVLIVMAVSLLSLGFRTGVVVLVIIPVVLAITFMFMHLF
                     DIGLHKISLGALIIALGLLVDDAIIAVEMMASKMEQGWGRVRAASYAWTSTAMPMLSG
                     TLVTAAGFLPVALAASSVGEFTRSIFQVTVTALLVSWIAAVLFVPYLGYYLLPDFSKR
                     AGQPSRLARVVGRVWPALGRRMAERDLHAHEQHDEFAIYHTPFYNRLRALVNACVRWR
                     WGVILLTVAIFVGALFVFARFVPQQFFPDSTRPELLVDLRLAEGASHHATEVAVKRLE
                     AFLDRQEGIENYVAWVGTGSPRFYLPLDQQLAQTNFAQLVILTAGLAEREALRAKLIH
                     LFEDEPWPLRGIVVRLENGPPVGFPVQYRVSGPDLAELHRRAQAVAEAMRANPHLSNV
                     HMDWVEPSKVVRVAIDQDKARLLGVSTQDLSSLLATSLQGMTATQFREGNKTIALVLR
                     GAESERAHLSQLPDLALPVGGGRSVPLAQVARLEHDFEPGVIWRRNRIPTVTVRASLY
                     DGTQPAVVVGQLAPRMAELSAQLPLGYRLEVGGAVEESAKGSGSVGAGMPIFVLTVLT
                     VLMLQLKSFSRTIIVVLTAPLGLIGVAAFLLAFGKPFGFVALLGTIALSGMIMRNSVI
                     LVDQIRQDVEAGRMQWEAIVESAVRRFRPIMLTAAAAILAMVPLSRSTFFGPMAVAIM
                     GGLTVATVLTILFLPALYAAWYRVKPADEERAAADRSAATALE"
     gene            8290..8943
                     /locus_tag="PKN25_07270"
     CDS             8290..8943
                     /locus_tag="PKN25_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNV90734.1"
                     /translation="MNFEKARFNMVEQQIRPWEVLDQDVLDLLMTVKREEFVPAAHRE
                     LAFADVEIPIGCGQVMLKPVIEGKILQALRVTKSDTVLEIGAGSGWFAALLAARAEWV
                     RTMEIEPELVKLASANLERNGVHNVVVVDGDGVQGWSGRAPYDVIVVSGGLPFVPQSL
                     LEQLKVGGRLFAFVGEAPVMKARLITCEGEGRFRTEDVFETVVPMLKNAPQREAFSF"
     gene            8951..9274
                     /locus_tag="PKN25_07275"
     CDS             8951..9274
                     /locus_tag="PKN25_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV90735.1"
                     /translation="MLQITPTMLAERLQDARRPAPLLLDVREPWEVQICQIPGSVTMP
                     MGSVPARYAELERDRETVVICHHGGRSWQVAMFLERQGFSNVANLSGGVAGWANEVDR
                     NMPQY"
     gene            9303..10607
                     /locus_tag="PKN25_07280"
     CDS             9303..10607
                     /locus_tag="PKN25_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNV90736.1"
                     /translation="MKRVLAVLVAGLFAGAAAAADLMQVYQDALANDAKFSAARAQLE
                     AGQEKVVQGRAGLLPQVGMDANTTRNDAKLKPVGASPREGSYNSNGYGVQLTQPLFRW
                     QNWVQFKQGELQTALAETQFGIARQDLVLRVAEAYFNVLNAQDALAAVTQLRTAAGEQ
                     LELAKTSFEVGTVTITDVHEAQSRFDLASAQEIAAQNLLEVARQTLAQIIGKQPEALA
                     GLREGVALQRPQPENIVDWVAAAESGGFGVQAQQLVREIAVREVERARAGHLPTVDVV
                     ARHGVNNDLQLSAGRNEVSSVGLQLNVPLFAGGRVSSVSREAAALRMKADADLEDARR
                     SAALAARQSWLGVTSGMAQVKALEAARVSSTSALDANKLGYEVGVRINIDVLNAQTQL
                     ADTLQKLARARYDTLLAQLRLKAAAGTLGEDDVRAINALLAP"
     gene            complement(10618..11889)
                     /gene="waaA"
                     /locus_tag="PKN25_07285"
     CDS             complement(10618..11889)
                     /gene="waaA"
                     /locus_tag="PKN25_07285"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HNV90737.1"
                     /translation="MLTRLPYTLLWILALPLVLLRLAWRARHQPAYLRHVGERFGRYR
                     TRAPVGVIWVHAVSVGETRAAEPLVRALLAEWPEHSVLLTHMTPTGRDTSKTLFRDEP
                     RVLRAYLPYDLGCFAHAFLRHFRPLFGVVMETELWPNLLAACRRRRIPVMLANARLSE
                     RSARRYARLPALTALTLKALAAIGAQTAADAARLAQLGARRVTVTGNLKFDIAPPAPL
                     LALGRSLRERIGARPVLLAASTREGEEALLLDAFARLAPPAALLLLVPRHPQRFDEVA
                     ALARARGLALQRRSADDAVRADTRVLLGDSMGEMFAYYAAADVALIGGSWLAFGGQNL
                     IEACAVGTAVVIGPHTFNFQAVAEDAERAGAAVRAQDAEAGMREALALVADPERRAQI
                     AAAGRAFATTHQGATARSVALLTALAARREA"
     gene            12033..13040
                     /locus_tag="PKN25_07290"
     CDS             12033..13040
                     /locus_tag="PKN25_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNV90738.1"
                     /translation="MKILVTGAAGFIGMHTTLRLLAGGDEVVGLDNLNDYYDPTLKEA
                     RLARLQPHAGFRFVKMDVADRAGMEALFAAEKFDRVIHLAAQAGVRYSLQNPHAYVDS
                     NLVGFMNILEGCRHAKVQHLVYASSSSVYGGNTKMPFSESDSVDHPVSIYAATKKANE
                     LMAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRPIDVFNHGRMRRDF
                     TYVDDIVEGVIRTLDRIAEPDPAFDPMQPNPGTGSAPYRVFNIGNHDPVELMEFVAAI
                     EDALGTTAQKNFLPLQDGDVPATYADTAALNAWTGFAPATSVREGVGRFIAWYREYYR
                     V"
     gene            13130..13927
                     /locus_tag="PKN25_07295"
     CDS             13130..13927
                     /locus_tag="PKN25_07295"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNV90739.1"
                     /translation="MANYAIGDIQGCYAPLDRMLDRIAFDPAVDRLWIVGDLVNRGPE
                     SLRVLRFLRGLGDAASIVLGNHDLYLLMVAAGYKRRDSDDTLFQVLEADDRDELLEWL
                     SFQPLMRVHGDHVLVHAGLLPGWTVTRAQALAAEVGAVLAGPKSREFLLHLAGNRPDR
                     WSDGLKGWDRLRVIVNAMTRLRFCTAQGRMDLSAKGAPHKAPSGMLPWFRVPDRFSRT
                     HTIVCGHWSALGFYREPGLIALDSGCVWGGKLTALRIEDDAVFQVPG"
     gene            complement(13944..14777)
                     /locus_tag="PKN25_07300"
     CDS             complement(13944..14777)
                     /locus_tag="PKN25_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV90740.1"
                     /translation="MHAGAGTAPPASASGAATRAAAAREARAGGLLRATRWTRLALHL
                     LQGVLTIATIYPLTGRDTREALRQRWSQGLLRVLGMRLEHRGEAIAPGCMLVANHVSW
                     VDIFAINALAPAAFVSKSEVRAWPVIGWLAAKNDTIFLRRGSRGHARIINEETAALLD
                     AGCNVAIFPEGTTTDGATLLHFHAALLQPAIACGHPVQPLALQYRTPDDRFSRAPAYD
                     GELSLGECIANIIAAPRTIARITVAAPIATADGADRRTLAARTRGEIATAIGVALDAQ
                     T"
     gene            complement(14816..15583)
                     /locus_tag="PKN25_07305"
     CDS             complement(14816..15583)
                     /locus_tag="PKN25_07305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HNV90741.1"
                     /translation="MLQHLQPRQQTAARKTRNLHVGLASSETEILEAQKLRYRVFAEE
                     MGARLPTRSPGVDRDLYDPFCEHLIVRDEDAGRIVGTYRILSPSAARRVGGYYSENEF
                     DLTRLQHLRGQLVEIGRSCIDADYRSGAVIALLWSGLARYMQENGYEYLIGCASVSMA
                     DGGHAAASLYNRLREKHLAPLEYHVFPRCPLPLSSLRGDLDAEAPPLIKGYLRAGAWV
                     CGEPAWDPDFNTADLPILMPIGRVDGRYARHFMGRKD"
     gene            15695..16144
                     /locus_tag="PKN25_07310"
     CDS             15695..16144
                     /locus_tag="PKN25_07310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNV90742.1"
                     /translation="MQCTTPPDHDPDCVFCRIARGELPSSKVYEDADTLAIMDIQSVN
                     PGHMLVLVKPHRANIYALDDTLAGAAFRTAARVARVAKKVTGCEGVTLLQANEKAGAQ
                     TVFHFHLHVLPRWEGDGMALAWPVKNPPREALEAMAAKLREALAGQD"
     gene            16235..17170
                     /locus_tag="PKN25_07315"
     CDS             16235..17170
                     /locus_tag="PKN25_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNV90743.1"
                     /translation="MSILVCGSVAYDSIMVFHDRFRNHILPEQIHILNVSFLVPDLRR
                     EFGGCAGNIAYGLKLLGSDPLIVATVGEDAGPYRARLRALGLREDFVREVPGTFTAQA
                     FITTDLDDNQITAFHPGAMFHSHLNDVRTAAGATLGIVSPDGADGMLRHADGFAAAGV
                     PFVFDPGQALPILGSEALLKCLQQARYCTVNDYEARLMCEKTGRSEAELAREVEALVV
                     TLGAEGSRVHWRGEVFEVPAVRADAIVDPTGCGDAYRAGLLHGIAAGWDLRRSARLAA
                     VMGALKIAQRGGQNYAPSRADVGARYREAFGEDLW"
     gene            17164..17637
                     /locus_tag="PKN25_07320"
     CDS             17164..17637
                     /locus_tag="PKN25_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNV90744.1"
                     /translation="MVNRSGMKWRALAGAMIAALAVGGCAQGLGGGTYARTEARRAMT
                     VQFGTIEAVRAVQLEGTKTPIGSVAGAAIGGIAGNTVGGGRGQAVATVIGAVAGGLAG
                     SAVEEGATRQAGVEVTVRLDNDQFLAVVQADEGEGFRPGERVRVLRDAGTTRVSR"
     gene            complement(17724..18920)
                     /locus_tag="PKN25_07325"
     CDS             complement(17724..18920)
                     /locus_tag="PKN25_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HNV90745.1"
                     /translation="MMITRCPACQTVFRLRPEQLHARGGEVRCGHCFHPFNALEHTVE
                     VRDDGAATTAAPPPPAARPVYEARPDPLLDFDIPEVAPSPPIRAPLPEPGLDDYEDWL
                     APAHKPALRGTAMDDGQTGTAPAFDEGAAAAQPFPEVLRSARRGLPIDEDEGGTPARG
                     RDLAAPGPFAPTISYTPPPPFDDDPATAIPGLSTPVRESARAAPEPASIPAHEDEQPA
                     SPHEPDLARLDANYGRPRKETHPLVRALAMLLAVGLSALLAAQATYLYRMELARALPG
                     LRPVLSEACARFGCGVPYPRDAEHIAIEASDLQAEPGRPGYYLLQATLNNRADYPQQW
                     PHLELTLTDAGDNPISRRVLPSTDWLPPSQPHEAFMARSAIELRIPFAAPALAPTGYR
                     IYAFYP"
     gene            complement(18917..19819)
                     /gene="prmA"
                     /locus_tag="PKN25_07330"
     CDS             complement(18917..19819)
                     /gene="prmA"
                     /locus_tag="PKN25_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNV90746.1"
                     /translation="MWISVTLQAEADKAEALSDALMEAGALSVSIEDADAGTEAERPQ
                     FGEPGHLPTALWDHSRVIALFDGAADPAELGAMLAEAARAAGFDAVPPFSTETVAEQN
                     WVQLTQSQFDPIRITDRLWIVPSWHEAPDADAINIELDPGMAFGTGSHPTTRLCLEWL
                     CEVVTPGCSVLDYGCGSGILGIAAAKLGAGEVLGIDIDEKAVEAARDNAARNHAAVRL
                     QHSAVPVGDTFDLVVANILTNPLCVLAPAISARVAPGGRVALSGVLETQAAQVVEAWA
                     PHIALHVGAAHEGWIRLEGRRPGP"
     gene            complement(19826..>20730)
                     /locus_tag="PKN25_07335"
     CDS             complement(19826..>20730)
                     /locus_tag="PKN25_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNV90747.1"
                     /translation="GYPVIIKAAGGGGGRGMRVVHTEAALLNAVTTTRAEAQAAFGNP
                     VVYMEKFLENPRHVEIQILADQHGNAVYLGERDCSMQRRHQKVIEEAPAPGIDRKTIA
                     KVGERCAEACRKIGYRGAGTFEFLYENGEFYFIEMNTRVQVEHPVTELITGIDIVQAQ
                     IRIAAGEKLWFRQRDIEFRGHAIECRINAEDPFKFTPSPGRITNWHTPGGPGIRVDSH
                     VYNGYTVPPHYDSMIGKLIAYGDTREQAIRRMRIALSEMMVEGIKTNIPLHQTLMLDP
                     RFNEGCTSIHYLEHKLTHRNEVQD"
BASE COUNT         2879 a         7066 c         7653 g         3132 t
ORIGIN      
        1 gaggtgaccg ccgagttgcc gatgttggcg ttgatcttgg tgaggaagtt gcggccgatg
       61 atcatcggct cgctctcggg gtggttgatg ttgttgggga tgatggcgcg cccgcgggcg
      121 atctcgtcgc gcacgaactc gggggtgatc gtcgccggca ggctggcgcc gaagttctgc
      181 ccggggtgct ggcgcagcat catgtcggcc atcttctggc ccttgaggcc gccgctggcg
      241 cgcagcgagg cgatgtactg ctcgcggttc aggttctcgc ggatggcgac gaattccatc
      301 tcgggcgtga tgatgccgcg gcgggcgtag tgcatctggg tgacattcgc accggccttg
      361 gcgcggcgcg gcttgcggtg caggccgggg aagcgcagct cgtccagggt cttgtcggcg
      421 gcgcgctggc ggccgaattc ggacgaaaga tcgggcagca gctcggtgtc gccacgctcc
      481 tcgatccact gctggcgcag cgcgggcagg ccggagcgga tgtcgatctt cgccgccggg
      541 tcggagtagg ggccggaaca gtcgtagacg aagatcggcg ggttctgctc ggcgccgaag
      601 gaggccgggg tgtcggcctg ggtgatctcg cgcatcggca cgcggatgtc ggggcgcgag
      661 ccctcgacgt ggatcttgcg cgagttgggc agcggggcga tcgcggcctc gtcgacgtgg
      721 gccttggcgg cgatgaattt ttcgttggcg ttcatgctgt ctcctggggg cttacttgcg
      781 ccacagcgcg tggaagtgat gcaccggccc gtggccgctg ccgaccgcga gttcgccgga
      841 gcgcgcgatc gcgcccagca accactggcg ggcgtggcgc accgcggtct cgaccgggcg
      901 gaactggcct tcggcctgcg gcagcagcgc ggcgatcgcg gagctgagcg tgcacccggt
      961 gccgtgggtg ttcttcgttt cgatgcgttg tgccggcagc tcgaccatgc ggtcgccgtc
     1021 gaacagcagg tcgaccaggt cgttgccggg caggtggccg cccttcagca gcacccagcg
     1081 ctcggaggac agcggcagga gctcgcgcag gcgctcggcg gcgcggtaca tctccttgac
     1141 cgtgtcgggt gcgcgccgct cgagcagcac gccggcctcg ggcaggttgg gggtgatcag
     1201 gaaggcctgc gggaagagcg cctcgcgcag cgccgagatc gcgctcttcg ccagcaggct
     1261 gtcgccgctc ttggccacca tgacggggtc gagcaccacc ctggccgcct gccagtgcgc
     1321 caggcgctcg gccaccgtgg tggtgacctc ggcgctgccg agcatgccga tcttggtggc
     1381 gtggatggcg acgtcctcga acagggtgtc gatctgcagg cgcaggaagt cggtgggcgg
     1441 cacatgcacg ccggtgacgg tgcgggtgtt ctgcgcggta agtgcggcga ccacgccgca
     1501 gccgtaggcg ccgagcgcgg aaaaggtctt gaggtcggcg aacacgccgg cgccgccgga
     1561 cgggtcgacg ccggcgatgg acaccacgtt cgggatgcgg gaagccgtcc cggacgatgc
     1621 ggacggcgcg ctgccggggg cagcggagct tgcggacgaa ctcatgaacg acgtttccct
     1681 acgccggtgc gagccggatc aggttccaag ggtgtttctc agcccgtgcg cggcgggcac
     1741 ccctaacgag agcgtgggaa gatactgtat gcactttcgc gtggcaagcc gctcaagatc
     1801 ccgccctcgc ggtcgtttag caggatggaa cgccatccca tgccgacgcc gatgaaaaac
     1861 ccgcgccctc tccttcttgc cgtgctgctc gcccatgtcg ccggcgcggt ctgcgccgcc
     1921 gactggcagc tcgtgttgag cgaccgcgac cgccgcatcg agatcgatcg cgcgagcgtc
     1981 ctccgctcgg acgccggcac caagatctcc tgggggcggg tggtgctgac ctcggaggag
     2041 gcgcggcgct ccggctatgc catgatcaag gcgctcaacc gctacgactg ccgcaaccgc
     2101 agcttcgcca cggtcaagcg ggtgtatgtc gatgccgacg acaacatcgt gcgcgaggag
     2161 ccggtggcgg ccgaggcgcc gatcgcggtg gcgcgcaaca cggtggacga gcgcatgtgg
     2221 cgcgaggtgt gcgatccgcc ctcggcggtc gagctgcaga agatggcggc cgaggcggcc
     2281 cggatcgcga gcgcgcagcc ggcggcggtg gcggcccctg ggggcgccgc agcgcccgcg
     2341 gctgcggcgg tttcgacgcc ccctgccgcg gccgccgcca cggccgtgca gtcgcctgcg
     2401 gcgaagcccg cggagtctgc gcgcgcgcag tccggcagcg tgcgccagcc gagcgcggag
     2461 tccgtctccg ccgaggtggt gcgtaccggt gccggcggtg agcagcccgc cgcggcgccc
     2521 cccgccttcg agacccacgt cgagatcccg gccaagttcc tgcctggcgg tcagggcgcc
     2581 gcggcgcccg agtcctctcg caagtccatt ctgccgccgc tgcccaagtt gtcggcgacg
     2641 gccgagcccg aggcgaagac cgcgccggcc cccaagccgc cggtgcgcga acagccgcct
     2701 gcgcaggggc gcgctgcgcc cgctccgcag gcggctgtgc cggcccgcca ggcggcgcca
     2761 gcgccggagc ggccggtcgc gccggagcgc gcgggcgcac gcgagaaggc gtccccggcc
     2821 agggccgcgt ccgccgtgcc ggcaagaccg acgccgccgc gcccgcgtcc cgccggtccg
     2881 gaacgcgtcg ccgcagcgca gccggcatcg ccggacgcga ctcccgcgcc gcccgagcgc
     2941 gccgcgtcgg cccgcgagga ggccttgcgc gcggccggca tcgagctcac ccgcctcggc
     3001 ccgggggtgc cggagtggag ctacgagggc gagcgcggtc cgcagcactg gggccgcatg
     3061 cgccccgagt ggcggctctg cgaggagggc acgcggcagt ctccgatcga cctgcgcgac
     3121 ggcatcgcgg tcgatctcgc gccggtgcgc ttcgactacc gtcgcaccgg cttccgcatc
     3181 cgcgataccg gcaacacctt gcaggtcgag gttggcgagg gcatgggcat caccgtgcgt
     3241 ggggtgcgtt acgcgctcga gcgcctcacc ctgcaccgcc cctcgcagga tcgcgtcggc
     3301 ggcatggcgc acgacatggc gatctacctg cagcatcgcg ccgatgacgg ccgcatggcg
     3361 atcgtctcgc tgctgctgtc ggcgggcggc gacgccagcc cggcgctgca gaccttgtgg
     3421 aacaacctgc cgctcgatcg cgggcgcgag ttcgttcccg acgccgtgct cgacctgccg
     3481 gcgctggtgc cggccgaccc gggccactac ctctacaccg gctcgttgcc gatgccgccg
     3541 tgtaccgagg acgtgctgtg ggtggtgatg aagcagccgg tgacgatctc cgcggaccag
     3601 ctcgacgtct tcgcccgcct gtacccgcgc aacggccggc cgatccagcc gaccaacggc
     3661 cggccgctgc tcgagtcgcg ctgagcgtcg tccgaccgcg aagagcccca tttgcgcact
     3721 tcaggatccc cggaggccgg atcgggtggc gggaacgacc cgcgatgggc aaggtccgcg
     3781 cgccggcggg gcgtcggcga tcatggccac acgggcgagg cgctacacta gcggcctcgc
     3841 ctcgcgtccg cgggttgtcg gcaggggcgc tcccgatcgc cacgagattt ccatgcgttt
     3901 ccgcaccctc ccgacgctgc tggccggcct gttcgtgctg gccgcctgtt ccgcgcccgc
     3961 accccatacc ccgccgccgc ccgcggtcct cgttcgcagc ttcgctgccg ccaatgcacc
     4021 cccgtccgtg caggtctatg ccggcgaggt gagggcgcgc gtcgagtccg acctgggatt
     4081 tcgcatcggc ggcaagctgg tcgagcgcct cgtcgatgtc ggcgccgagg ttgccgccgg
     4141 cacgccgctg gcgctgctcg acgcgcagga cgtgcgcctg gcgctggctt cggcgcaggc
     4201 cgcggccgcg gccgccgagt ccgacctcgc gcttgcgcgc acggaattcg cccgcgcacg
     4261 cgagctgcag gcgcgcaact tcgtctcgtc ctcgctgctc gacgcccgtc gcaccgcgct
     4321 gcaggccgcc gaggcgcgcc ttcgccaggc gcgcgcgcag gccgaggtcg ccgccaacca
     4381 ggccggctac gcccgcctcg aggcgcccgc cgccggcgtg gtcaccgcgg tgctcgccga
     4441 gccggggcag gtggtcgggg cgggccaggc ggtgctgcgc ctcgcccgcc ccgacgagcg
     4501 cgaggtgctg atccacgtgc ccgaggggcg caccggccgc atcgcgccgg gcagcgccgc
     4561 gcaggtgcgc ccatgggcgg cacccgagcg cgcatacgcc ggccgcgtgc gcgaggtcgc
     4621 gccggcggcc gacgctgcca cccgcaccca cgcggtgcgg gtcgcggtgc cgggggccga
     4681 cgccacgctc gcgctcggcg ccagcgcgag cgtggccttc gccgcggcgg atggcgaggc
     4741 cggcggcgtg ctgctgccgc tgcccgcggt gacgcgacaa cccggccagg ccgatgccgg
     4801 cacggtgtgg gtggtgggcg aggacgacgc cgtgcggccg ctcacggtcg agctcggcgc
     4861 ctggcgcgag gacggcgtcg tggtgcgcgc cggcctgccg gcgcaggccc gtgtcgtggt
     4921 ggccggggtg tacaagctga tcgagggcat gcgcgtgcgt gccgtcgagg aaggcgcgcc
     4981 ggtgctgctg gacgtgcggc gatgagcatg aagggcgagg ggccgatcgg cagcgggcgc
     5041 ttcaacctgt ccgaatgggg gctgacccac cgctccctgg tgctgtactt cctgatcgtg
     5101 tgcacgctga gcgggatgtt cgcctacacc cggctcggcc agtccgagga tccgcccttc
     5161 accttcaagg tcatggtggt gcgcgccgag tggccggggg cgagtgcgcg cgaggtcgaa
     5221 cagcaggtca ccgacaagat cgagaagaag ctgcaggaga tcgcccaggc cgacatcatc
     5281 cgcagctact ccaagccggg cgaggcgatg gtcttcttcg tggcgaagga ctccacgcgc
     5341 gcgcgcgaca tccccgacat ctggtaccag gcgcgcaaga agatcggcga catcgcccac
     5401 accttcccga ccggcgtgcg cggaccgtat ttcaacgacg agttcggcga cacttacggg
     5461 aatgtcttcg ccctgctcgg cgagggcgtg agccacgccg agctcgagcg ccacgccgag
     5521 gcgatccgcg gcgagctgct gcgcgtgaag gacgtcgcca aggtgagctt cttcgccgag
     5581 cagccgcagc gcgtcttcgt ggagatctcc aacacgcgcc tcgccaccct gggcgtgcgc
     5641 ctggacgaca tcgtcggcgc gctcgcccgg cagaacgcgg agaccggcgc cggcttcttc
     5701 gagaccgggg atgagcgcat ctggctgcgg ccgtcggggc agttcgagga cctcgacgcg
     5761 attcgcgcca ccgtgatccg cgccggcggg cgcagcctgc gcctgggcga catcgccgag
     5821 gtgcgccgcg gctacgccga cccgccggcc gagcgcctgc gcttcatggg gcaggaggcg
     5881 ctcggcatcg gtgtgtcgat gcgcagcggc ggcgacatcg tcgcgctcgg ccgccagctc
     5941 gacgcggcgg tggagaagat cgtcgcgcag ttgcccgtcg gcatccgcct cgagcgcgtg
     6001 gccgaccagc cgcgtgcggt cacgcgggcg gtcgacgagt tcgtcaaggt gctcgccgag
     6061 gcggtgctca tcgtgatggc ggtgagcctg ctgtcgctcg gcttccgcac cggcgtggtg
     6121 gtgctggtga tcatcccggt ggtgctggcg atcaccttca tgttcatgca cctgttcgac
     6181 atcggcctgc acaagatctc gctcggggcg ttgatcatcg cgctcggctt gttggtggac
     6241 gacgcgatca tcgccgtcga gatgatggcg agcaagatgg agcagggctg gggccgggtg
     6301 cgggcggcga gctacgcgtg gacctcgacc gcgatgccga tgctctcggg cacgctggtg
     6361 acggcagcgg gtttcctgcc ggtggcgctg gcggcgtcct cggtgggcga gttcacgcgc
     6421 tcgatcttcc aggtcacggt gaccgcgctg ctggtgtcgt ggatcgccgc ggtgctcttc
     6481 gtgccctatc tgggctacta cctgctgccc gacttcagca agcgcgccgg gcagccctcg
     6541 cggctggcgc gcgtggtcgg aagggtctgg ccggcgctgg ggcggcggat ggcggagcgc
     6601 gacctccatg cccacgagca gcacgacgag ttcgcgatct accacacgcc cttctacaac
     6661 cggctgcggg cgctggtgaa cgcctgcgtg cgctggcgct ggggggtgat cctgctcacc
     6721 gtggcgatct tcgtcggcgc gctgttcgtg ttcgcccgct tcgtgccgca gcagttcttc
     6781 cccgactcga cgcggcccga gctgctggtc gacctgcgcc tggccgaggg tgcgtcgcac
     6841 cacgccaccg aggtcgcggt gaagcggctg gaggccttcc tcgacaggca ggagggcatc
     6901 gagaactacg tggcctgggt gggcaccggc agcccgcgct tctacctgcc gctcgaccag
     6961 caactcgcgc agaccaactt cgcccagctc gtgatcctca ccgccggcct ggccgagcgc
     7021 gaggcgctgc gcgcgaaact gatccacctc ttcgaggacg agccctggcc gctgcgcggc
     7081 atcgtcgtcc ggctggagaa cgggccgccg gtgggctttc cggtgcagta ccgggtgagc
     7141 gggccggatc tggccgagct gcaccggcgt gcgcaggcgg tggccgaggc gatgcgcgcc
     7201 aatccgcacc tttccaatgt gcatatggac tgggtggagc cgtccaaggt ggtgcgggtg
     7261 gcgatcgacc aggacaaggc gcgcctgctc ggcgtctcca cccaggacct ctccagtctg
     7321 ctcgccacct cgctgcaggg catgaccgca acccagttcc gcgagggcaa caagaccatc
     7381 gcgctggtgc tgcgcggtgc ggagagcgag cgcgcgcacc tgtcgcagct gcccgacctc
     7441 gccctgccgg tgggcggcgg gcgcagcgtg cccttggcgc aggtggcgcg cctcgagcac
     7501 gacttcgagc cgggggtgat ctggcggcgc aaccgcatcc ccaccgtgac cgtgcgcgcc
     7561 tcgctctacg acggcaccca gcccgcggtg gtggtgggcc agctcgcgcc gcgcatggcc
     7621 gagctctccg cgcagctgcc gctcggctac cgcctcgagg tcggcggcgc ggtcgaggag
     7681 agcgccaagg gcagcggctc ggtgggcgcc ggcatgccga tcttcgtgct caccgtgctc
     7741 acggtgctga tgctgcagct gaagagcttc tcgcgcacca tcatcgtcgt gctcaccgca
     7801 ccgctcggcc tgatcggtgt cgccgccttc ctgctcgcct tcggcaagcc cttcggcttc
     7861 gtcgccctgc tcggcacgat cgcgctgtcg ggcatgatca tgcgcaactc ggtgatcctg
     7921 gtggaccaga tccgccagga cgtcgaggcc gggcgcatgc agtgggaggc catcgtcgag
     7981 tccgcggtgc ggcgcttccg cccgatcatg ctcaccgccg cggcggccat cctggccatg
     8041 gtgcccttgt cgcgcagcac cttcttcggg ccgatggcgg tggcgatcat gggcgggctg
     8101 accgtcgcca cggtgctcac catcctcttc ctgcccgcgc tctatgccgc ctggtaccgt
     8161 gtcaagccgg cggacgagga gcgcgcggcg gcggatcgca gcgcagcaac tgcgctagaa
     8221 tgacgaggtt ttgccttcat ggataacgct atccattagc gtgtgctaat atttcgccgg
     8281 gagcccctga tgaacttcga gaaagcgcgc ttcaacatgg tcgagcagca gatccgcccc
     8341 tgggaggtgc tggatcagga cgtgctcgac ctcctgatga cggtcaagcg cgaggaattc
     8401 gttcccgccg cgcatcgcga gctggccttc gccgacgtcg agatcccgat cggctgtggc
     8461 caggtcatgc tcaagccggt gatcgaaggc aagatcctgc aggcgctgcg tgtcaccaag
     8521 tccgacaccg tgctcgagat cggcgccggc tcgggctggt tcgccgcgct gctggcggcg
     8581 cgtgccgagt gggtgcgcac catggagatc gagcccgagc tggtgaagct ggcgagcgcc
     8641 aacctcgagc gcaacggcgt gcacaacgtg gtcgtggtcg acggcgacgg tgtgcaggga
     8701 tggtctggcc gcgcgcccta cgacgtgatc gtcgtttccg gcgggctgcc cttcgtgccg
     8761 cagtcgctgc tcgagcagct caaggtgggt ggtcgcctgt tcgccttcgt cggcgaggcg
     8821 ccggtcatga aggcgcgcct gatcacctgt gagggcgaag gccgcttccg taccgaggac
     8881 gtgttcgaga ccgtggtgcc catgctcaag aacgcgcccc agcgcgaagc cttcagcttc
     8941 tgaggcgacg atgctgcaga tcacgccgac catgctcgcc gagcgcctgc aagacgcgcg
     9001 acggcctgca cccctgctgc tcgacgtgcg cgagccgtgg gaggtgcaga tctgccagat
     9061 ccccggctcg gtgacgatgc cgatgggcag cgtcccggcg cgttacgcgg agctggaacg
     9121 cgaccgcgag acggtggtca tctgccatca cggcggacgc agctggcagg tcgcgatgtt
     9181 cctcgagcgc cagggcttct ccaacgtggc caacctgtcc ggcggcgtcg ccggctgggc
     9241 gaacgaggtc gatcgcaaca tgccgcagta ttgagcgcgg cgcagcagca gcggagaaca
     9301 cgatgaagcg agtcctggca gttctggtgg cgggcctgtt cgcgggcgct gccgcggcgg
     9361 ccgacctgat gcaggtgtac caggacgcgc tcgccaacga cgccaagttc tccgccgcgc
     9421 gcgcccagct cgaggccggg caggagaagg tggtgcaggg ccgcgccggg ctgctgccgc
     9481 aggtgggcat ggacgccaac accacccgga acgacgccaa gctcaagccg gtcggggcct
     9541 cgccccgtga aggcagctac aacagcaacg gctacggcgt gcagcttacc cagccgctgt
     9601 tccgctggca gaactgggtg cagttcaagc agggtgagct gcagactgcg ctcgccgaga
     9661 cccagttcgg cattgcacgc caggatctcg tgctgcgcgt cgccgaggcc tacttcaacg
     9721 tgctcaacgc ccaggacgcg ctcgccgcgg tgacgcagct gcgcaccgcg gccggcgagc
     9781 agctcgagct ggcgaagacc agcttcgagg tcggcacggt gacgatcacc gacgtgcatg
     9841 aggcgcagtc gcgcttcgac ctcgcctcgg cgcaggagat cgcggcgcag aacctgctcg
     9901 aggtcgcgcg ccagaccttg gcgcagatca tcggcaaaca acccgaggcg ctggccggcc
     9961 tgcgcgaagg cgtcgcgctg cagcgcccgc aacccgagaa catcgtcgac tgggtggccg
    10021 cggccgaatc cggcggcttc ggcgtgcagg cccagcagct ggtgcgcgag atcgccgtgc
    10081 gcgaagtcga acgcgcccgc gccggccatc tgcctacggt cgatgtcgtc gcccggcacg
    10141 gtgtcaacaa cgacctgcaa ctctcggccg gacgcaatga agtgagcagt gtcggcctgc
    10201 agctcaacgt gcccttgttt gcgggcggcc gggtgtcctc ggtgtcgcgc gaggccgctg
    10261 cgctgcgcat gaaggccgat gccgatctcg aggacgcccg tcgcagcgcc gcgctcgccg
    10321 cacgccagtc ctggctgggc gtgaccagcg gcatggcgca ggtgaaggcg ctcgaggccg
    10381 cgcgcgtctc gtccacctcg gcgctcgacg ccaacaagct cggctacgag gtcggcgtgc
    10441 gcatcaacat cgacgtgttg aacgcccaga cccagctcgc cgacaccttg cagaagctcg
    10501 ctcgtgcgcg ctacgacact ctgctcgcgc agttgcgcct gaaggccgcc gccggcaccc
    10561 tgggcgagga cgacgtgcgc gcgatcaacg cactgctcgc gccctgaacg gcacggctca
    10621 cgcctcacgc ctcgcagcca gcgccgtgag cagcgcaacg ctgcgcgcgg tcgcgccttg
    10681 gtgcgtggtc gcgaaggcgc gccccgcggc ggcgatctgc gcgcgtcgct ccgggtcggc
    10741 gaccagcgcc agcgcctcgc gcatgccggc ctccgcatcc tgcgcgcgca ccgcggcacc
    10801 ggcgcgctcg gcgtcctcgg ccaccgcctg gaaattgaag gtgtgcgggc cgatcaccac
    10861 cgcggtgccg accgcgcagg cctcgatcag gttctgcccg ccgaaggcca gccagctgcc
    10921 gccgatcagg gcgacgtcgg cggcggcgta gtaggcgaac atctcgccca tcgagtcgcc
    10981 gagcagcacg cgcgtgtccg cacgcaccgc gtcgtccgcg ctgcgccgct gcagcgcgag
    11041 gccgcgcgcg cgggcgagcg cggcgacctc gtcgaagcgc tgcgggtggc gcggcaccag
    11101 cagcagcaag gccgcgggcg gcgccaggcg ggcgaaggcg tcgagcagca gggcctcctc
    11161 tccctcgcgg gtgctcgcgg cgagcagcac cgggcgcgcg ccgatgcgtt cgcgcaggct
    11221 gcggccgagc gccagcagcg gcgccggcgg ggcgatgtcg aacttgaggt tgccggtgac
    11281 cgtgactcgg cgcgcgccga gttgggcgag gcgggcggcg tcagcggcgg tctgcgcgcc
    11341 gatcgccgcg agtgccttca gggtgagcgc ggtaagcgcg ggcaggcggg cgtagcgacg
    11401 cgccgagcgc tccgaaagac gggcgttggc aagcatcacc ggaatgcggc ggcgccggca
    11461 ggcggcgagg aggttgggcc acagctcggt ctccatcacc actccgaaca gcgggcggaa
    11521 gtggcgcagg aaggcgtggg cgaagcagcc gaggtcgtag ggcaggtagg cgcgcagcac
    11581 acgcggctcg tcgcggaaga gggttttcga ggtgtcgcga ccggtcggcg tcatgtgggt
    11641 gagcagcacc gagtgctccg gccattcggc gagcagggcg cgcaccagcg gttcggcggc
    11701 gcgggtctcg cccacggaga ccgcgtgcac ccagatcacc cccaccggcg cgcgcgtccg
    11761 gtagcgcccg aagcgctcgc cgacgtggcg cagataggcg ggctggtggc gcgcccgcca
    11821 ggccaggcgc agcagcacca ggggcagggc gaggatccac agcaaggtat agggcaggcg
    11881 ggtcagcatc gggtggaagg ggaggacgac cgccgcagta taagcaagga cgccggccgc
    11941 ggtcctgcgc gcgatgaagg aaaccgttca cactgtgcgc tggcgtgggc atgcgataat
    12001 ttgtccaatc catcgcccag ccaatttcct ccatgaagat cctcgtcacc ggcgctgccg
    12061 gttttatcgg catgcacacc acgctgcgcc tgctcgcggg cggcgacgag gtggtcggcc
    12121 tcgacaacct gaacgactac tacgacccca ccctcaagga ggcgcgcctt gcccggctgc
    12181 agccgcacgc gggcttccgc ttcgtgaaga tggacgtggc cgaccgcgcc ggcatggagg
    12241 ccttgttcgc cgccgagaag ttcgaccgcg tcatccacct cgccgcccag gccggggtgc
    12301 gctattcgct gcagaacccg cacgcctatg tcgacagcaa cctggtcggg ttcatgaaca
    12361 tcctcgaggg ctgccgccac gcgaaggtgc agcacctggt ctatgcgtcg agctccagcg
    12421 tgtatggcgg caacacgaag atgccgttct cggagagcga cagcgtcgac cacccggtga
    12481 gcatctacgc ggcgaccaag aaggccaacg agctgatggc gcacacctat agccacctct
    12541 acggcctgcc caccaccggg ctgcgcttct tcaccgtgta tggcccctgg ggccgcccgg
    12601 acatggcgct gttcctgttc accaaggcga tcctggaagg gcgtccgatc gatgtcttca
    12661 accacggccg catgcggcgc gacttcacct atgtcgacga catcgtcgag ggggtgatcc
    12721 gcacccttga ccgcatcgcc gaacccgatc ccgccttcga tccgatgcag ccgaacccgg
    12781 gcaccggcag cgcaccctac cgtgtgttca atatcggcaa ccatgacccg gtcgaactga
    12841 tggagttcgt cgccgcgatc gaggatgcgc tcggcaccac cgcgcagaag aacttcctgc
    12901 cgctgcagga cggcgacgtc ccggccacct acgccgacac cgccgcgctg aacgcctgga
    12961 ccggcttcgc cccggcgacc agcgtgcgcg agggcgtcgg ccgcttcatc gcctggtacc
    13021 gggagtacta ccgggtctga tccgcccggg gcggcccggc gcgacggccg ccccccgatc
    13081 tacaaccttc catgcgctgc ggcccgccgg ccggcagcgg caggaatcga tggccaacta
    13141 cgcaatcggc gacatccagg gctgctacgc cccgctggac cgcatgctcg accgcatcgc
    13201 cttcgatccc gcggtcgacc ggctgtggat cgtcggcgac ctcgtcaatc ggggccccga
    13261 atccctgcgt gtgctgcgtt tcctgcgcgg gctgggcgat gctgcgagca tcgtgctcgg
    13321 caaccacgac ctctacctgc tgatggtcgc cgccggctac aagcggcgcg acagcgacga
    13381 cacgctgttc caggtgctcg aggcggacga ccgcgacgag ttgctggagt ggctgagctt
    13441 ccagcccctg atgcgggtcc atggcgacca cgtgctggtg catgccggcc tcttgcccgg
    13501 gtggacggtg acgcgcgcgc aggcgctcgc cgccgaggtg ggtgcggtgc tggccgggcc
    13561 gaagtcgcgc gagttcctgc tccacctcgc gggcaaccgc ccggatcgct ggtcggacgg
    13621 gctcaagggc tgggaccgtc tgcgcgtgat cgtcaatgcc atgacgcgcc tgcgcttctg
    13681 taccgcgcag gggcgcatgg acctgagtgc caagggcgcg ccgcacaagg cgccgtcggg
    13741 catgctgcca tggttccgcg tgcccgaccg tttcagccgt acccatacga tcgtctgcgg
    13801 gcactggtcg gcgctcggct tctaccgcga gccggggctg atcgcgctcg attcgggctg
    13861 cgtgtggggc ggcaagctga ccgcgctgcg catcgaggac gacgcggtgt tccaggtccc
    13921 gggctgagcc tgcggcggtg gtctcaggtc tgcgcgtcga gcgcgacgcc gatggcggtg
    13981 gcgatctcgc cgcgcgtgcg tgcagccagc gtgcgccggt ccgctccgtc ggcggtggcg
    14041 atcggcgccg ccaccgtgat gcgcgcgatc gtgcgcggcg cggcgatgat gttggcgatg
    14101 cactcgccca ggctgagctc gccatcgtag gcgggcgcgc ggctgaagcg gtcgtccggc
    14161 gtgcggtatt gcagcgcgag gggctgcacc ggatggccgc aggcgatcgc cggctgcagc
    14221 agcgcggcgt ggaagtgcag cagggtcgcg ccgtcggtgg tcgtgccctc ggggaagatg
    14281 gcgacgttgc acccggcgtc gagcagcgcg gcggtttcct cgttgatgat gcgggcgtgg
    14341 ccacggctgc cgcgacgcag gaagatggtg tcgttcttcg ccgccagcca gccgatcacc
    14401 ggccaggcgc gtacctcgga cttggagacg aaggcggcgg gggcgagcgc gttgatcgcg
    14461 aagatgtcca cccaggagac gtggttggca accagcatgc aaccgggtgc gattgcctcg
    14521 ccgcggtgct cgaggcgcat cccgagcacc cgcagcagcc cctgagacca gcgctggcgc
    14581 agggcctcgc gagtgtcgcg accggtgagc ggatagatcg tcgcgatcgt cagcaccccc
    14641 tgcagtaggt gcagggcgag ccgggtccag cgcgtcgcgc gcagcagccc accggcgcgc
    14701 gcctcgcgcg ccgcggccgc gcgggtggct gctccggacg cggaggctgg tggcgcggtg
    14761 ccggcaccgg catgcatggc gcgatcctgc acgaggtcgg ggtggcgggg ggcgttcagt
    14821 ccttgcgtcc catgaagtgg cgggcgtagc gcccatccac gcggccgatc ggcatcagga
    14881 tgggcaggtc cgcggtgttg aagtcggggt cccatgccgg ctcgccgcag acccaggctc
    14941 ccgcgcgcag gtagcccttg atcagcggtg gcgcctcggc gtcgaggtcg ccgcgcaggc
    15001 tcgagagcgg cagggggcag cgcgggaaga cgtggtactc gagcggggcg aggtgttttt
    15061 cgcgcaggcg attatacagg ctggccgcgg cgtgcccgcc gtcggccatg ctcaccgagg
    15121 cgcagccgat caggtattcg tagccgttct cctgcatgta gcgcgcgagc cccgaccaca
    15181 gcagcgcgat caccgcgccg ctgcggtagt cggcgtcgat gcacgagcgc ccgatctcca
    15241 ccagctggcc gcgcaggtgc tgcaggcggg tgaggtcgaa ttcgttctcg gagtagtagc
    15301 cacccacccg gcgtgcggcg gagggcgaca ggatgcgata ggtgccgacg atgcggccgg
    15361 catcctcgtc gcgcacgatc aggtgctcgc agaagggatc gtagaggtcg cggtcgacgc
    15421 ccggactgcg tgtgggcagg cgggccccca tctcctcggc gaagacccgg tagcgcagct
    15481 tctgcgcctc gaggatctcg gtctcgctgc tggcgaggcc gacgtgcagg ttgcgcgtct
    15541 tgcgcgcggc ggtttgctgg cggggctgga ggtgctggag catggcctgt gtccgtcctg
    15601 ttgggtgcgg accattctcg aaacccgtcg ttgcgacgcg gtgacgtgct ggttacgatg
    15661 cgatgacgca catccggccc caggagaact tcgcatgcag tgcacgaccc cgcccgacca
    15721 cgaccccgac tgcgtgttct gccgcatcgc gcgcggcgaa ctgccgtcgt ccaaggtgta
    15781 tgaggacgcc gacacgctcg ccatcatgga catccagtcg gtcaatcccg gccacatgct
    15841 ggtgctggtc aagccgcacc gcgcgaacat ctacgcgctc gacgacaccc tcgccggcgc
    15901 ggccttccgc actgcggcgc gggtcgcccg cgtggcgaag aaggtgaccg gctgcgaggg
    15961 agtgacgctg ctccaggcca acgagaaggc gggggcgcag accgtgttcc acttccatct
    16021 ccacgtgctg ccgcgctggg aaggcgacgg catggcgctg gcctggccgg tgaagaaccc
    16081 gccgcgcgag gcgctcgagg cgatggcggc gaagctgcgc gaggcgctcg ccggtcagga
    16141 ctgaagacct ggccgcggct ccggcggcgg caggggaagg cggaggggcc gcgtataatc
    16201 cggctttcgc ccaccctccg cctgcctgcc ttccatgtcc atcctcgtct gcggatcggt
    16261 cgcctacgac tcgatcatgg tcttccacga ccgcttccgg aatcacatcc tccccgagca
    16321 gatccatatc ctcaacgtct ccttcctggt gcccgacctg cgccgggagt tcggcggctg
    16381 tgccggcaac atcgcctacg ggctcaagct gctcggcagc gatccgctga tcgtcgccac
    16441 cgtgggcgag gacgcaggtc cctaccgtgc gcgcctgcgc gcgctcgggc tgcgcgagga
    16501 cttcgtgcgc gaggtgccgg gcaccttcac cgcgcaggcc ttcatcacca ccgacctcga
    16561 cgacaaccag atcaccgcct tccatccggg ggcgatgttc cactcgcacc tgaacgacgt
    16621 gcgcaccgcg gccggtgcga cgctgggcat cgtctcgccc gacggcgccg acggcatgct
    16681 gcgccacgcc gacggcttcg ccgcggccgg ggtgcccttc gtgttcgacc cgggccaggc
    16741 cctgcccatc cttggtagcg aagcgttact gaaatgcctg caacaggcgc gctactgtac
    16801 ggtcaatgac tacgaggccc ggctgatgtg cgagaagacc gggcgcagcg aagcggagct
    16861 cgcccgcgag gtggaggcgc tggtggtgac cttgggagcg gaaggttcgc gcgtgcattg
    16921 gcgcggcgag gtgttcgagg tgccggcggt gagggcggac gcgatcgtcg acccgaccgg
    16981 ttgcggcgac gcctaccgtg ccggcctgct gcacggtatc gccgcgggct gggacctgcg
    17041 caggagcgcg cgcctggccg cggtcatggg cgcgctcaag atcgcccagc gtggcggaca
    17101 gaactacgcg ccttcgcgtg cagacgtcgg ggcgcgttat cgcgaggctt tcggagagga
    17161 tctatggtga atcgaagcgg gatgaagtgg cgcgccctgg ccggcgcgat gatcgcggcg
    17221 ctggcggtgg gcggctgcgc ccagggactg gggggtggca cgtatgcgcg caccgaggcg
    17281 cggcgcgcga tgacggtgca gttcggcacc atcgaggcgg tacgcgcggt gcagctcgag
    17341 ggaacgaaga cgccgatcgg ctcggtcgcg ggcgcggcca tcggcggtat cgccggcaac
    17401 acggtcggcg gcggacgtgg ccaggccgtg gccaccgtca tcggcgcggt ggccggcggg
    17461 cttgccggct ctgcggtgga ggaaggcgcg acccggcaag cgggcgtgga agtcaccgtg
    17521 cgtctggaca atgaccagtt tctcgccgtg gtgcaggccg acgagggcga aggcttccgc
    17581 cccggtgagc gcgtccgcgt gctgcgcgac gccggcacca cccgcgtctc gcgctgagaa
    17641 ggatgccagg cgcgagcccg gcgcatgcgg ggtcgagcgc gttcgctccc gctcctcacc
    17701 cctgatgcct tacgcctcac tcctcacgga tagaatgcat agatccggta tcccgtcggt
    17761 gccagcgccg gtgcggcgaa gggaatgcgc aactcgatcg cgctgcgtgc catgaaggct
    17821 tcgtgcggct ggctcggtgg cagccagtcg gtggatggca acacgcggcg cgagatcggg
    17881 ttgtcgccgg cgtcggtgag ggtgagttcc agatgcggcc actgctgcgg atagtccgcg
    17941 cggttgttga gcgtggcttg cagcaggtag tagccgggcc gcccgggttc ggcctgcagg
    18001 tcggaggcct cgatcgcgat gtgttccgca tcgcgcggat agggcacgcc gcagccgaag
    18061 cgcgcgcagg cctcgctcag gacggggcgc aggccgggga gggcgcgcgc cagctccatg
    18121 cgatacagat aggtggcctg cgccgcgagc agcgcggaca agccgaccgc gagcagcatc
    18181 gcgagcgcgc gtacgagcgg gtgggtttcc ttgcgcgggc gtccgtagtt ggcgtcgagg
    18241 cgggcgaggt cgggctcgtg aggcgaggcg ggttgttcat cctcgtgtgc agggatggag
    18301 gcgggttccg gtgcggcccg cgcggattcg cgcacggggg tggagagccc ggggattgcg
    18361 gtggccggat cgtcgtcgaa gggcggcggt ggcgtgtagg agatcgtcgg tgcgaagggc
    18421 ccgggcgcgg cgaggtccct gccgcgagca ggcgttccgc cctcgtcctc gtcgatcggc
    18481 aggcctcgcc gggcgctgcg caagacttcc ggaaagggct gggccgctgc ggcgccctcg
    18541 tcgaaggcgg gggccgtgcc ggtctgcccg tcgtccatcg cggtgccgcg cagcgcgggc
    18601 ttgtgcgcgg gcgcgagcca gtcctcgtag tcgtcgagac ccggctcggg cagcggcgcc
    18661 cggatcggcg gcgagggggc aacctcgggg atgtcgaagt cgagcagcgg atcggggcgt
    18721 gcttcataca ccggtctcgc cgcggggggt gggggcgccg ctgtggtcgc ggcgccgtcg
    18781 tcgcgaacct cgaccgtgtg ctcgagcgcg ttgaaggggt ggaagcaatg cccgcagcgg
    18841 acctcgccgc cccgtgcgtg aagctgctcc gggcgcagcc ggaacaccgt ctggcaggcg
    18901 gggcagcggg tgatcatcat ggcccggggc gccgtccctc gaggcggatc cagccttcgt
    18961 gcgccgcgcc cacgtgcagt gcgatgtgcg gtgcccaggc ctccacgacc tgcgcggcct
    19021 gggtctcgag cacgcccgac agcgccaccc ggccgcccgg agcgacccgc gccgaaatcg
    19081 ccggcgcgag cacgcacagc gggttggtga ggatgttggc gacgaccaga tcgaaggtgt
    19141 cgccgaccgg cacggcagag tgctgcaggc gtacggccgc gtggttgcgc gcggcgttgt
    19201 cgcgcgcggc ctcgaccgcc ttctcgtcga tgtcgatgcc gagcacctcg ccggcgccga
    19261 gcttggccgc ggcgatgccg aggatgcccg agccgcagcc gtagtcgagc acgctgcagc
    19321 cgggcgtgac cacctcgcac agccactcca ggcagagccg ggtggtgggg tgggagccgg
    19381 tgccgaaggc catgccgggg tcgagctcga tgttgatcgc gtcggcgtcg ggcgcctcgt
    19441 gccaggacgg cacgatccac agccggtcgg tgatgcggat cgggtcgaac tggctctggg
    19501 tgagctgcac ccagttctgc tcggctaccg tctcggtgga gaacgggggg acggcgtcga
    19561 aacccgccgc gcgggcggct tcggcgagca tcgcgccgag ctcggccgga tcggccgcac
    19621 cgtcgaacag cgcgatcacc cggctgtggt cccacagcgc ggtgggcagg tggccgggct
    19681 cgccgaactg cgggcgctcc gcctcggtgc cggcgtcggc gtcctcgatc gagacagaga
    19741 gcgcgccggc ctccatcagt gcgtcggaga gcgcctcggc cttgtcggcc tcggcctgca
    19801 gggtcaccga gatccacatc gcgcgtcagt cctgcacttc gttgcggtgg gtgagcttgt
    19861 gctcgaggta atggatgctg gtgcagccct cgttgaagcg cgggtcgagc atcagggtct
    19921 ggtgcagcgg gatgttggtc ttgatgccct cgaccatcat ctcggagagc gcgatgcgca
    19981 tgcgacggat cgcctgctcg cgagtgtcgc cgtatgcgat cagcttgccg atcatcgagt
    20041 cgtagtgggg cggcacggtg tagccgttgt aaacgtgcga atcgacccgg atccccggcc
    20101 cacctggcgt atgccagttg gtgatgcgac cgggcgaggg cgtgaacttg aacggatcct
    20161 cggcgttgat ccggcactcg atcgcgtggc cgcggaactc gatgtcgcgc tgacggaacc
    20221 agagcttctc gccggcggcg atgcggatct gcgcctgcac gatgtcgatg ccggtgatca
    20281 gttcggtcac cggatgctcc acctgcaccc gcgtgttcat ctcgatgaag tagaactcgc
    20341 cgttctcgta gaggaactcg aaggtgccgg cgccgcggta gccgatcttg cggcaggctt
    20401 ccgcgcaacg ttcgccgacc ttggcgatcg tcttgcggtc gatgccgggg gccggcgctt
    20461 cctcgatcac cttctggtgg cggcgctgca tggagcagtc gcgttcgccg agatagaccg
    20521 cgttgccgtg ctggtcggca aggatctgga tctcgacgtg gcgcggattc tcgaggaact
    20581 tctccatgta tacgacgggg ttgccgaagg ccgcctgggc ctcggcgcgg gtggtggtga
    20641 cggcgttgag cagcgcggcc tcggtgtgca ccacgcgcat gccgcgcccg ccgccgccgc
    20701 cggcggcctt gatgatcacc gggtagccga
//
LOCUS       DAOQBS010000048        19813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1884_length_19813_cov_13.512653, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000048 DAOQBS010000000
VERSION     DAOQBS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 19813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19813
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1884_length_19813_cov_13.512653"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..3365
                     /locus_tag="PKN25_07340"
     CDS             15..3365
                     /locus_tag="PKN25_07340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV90748.1"
                     /translation="MVRYILNRPGLLGERPNYSPDDLYWLHNAEVAEAVPHHEGVLHM
                     PAVDTSIFNNVDNPHDNNRQGACIYFGRYVEGAKAFPELTDRCTVITKDFPTSHEELA
                     ALFRRSTHVYCFENTSISMEARLCGCPVVQLPSPYADPDKPFADSLGLSDALVTSDDA
                     ETLAEARRLLPSLTQKYRRLEAAYWEELERLLDKTQAVAESRPKTHKSPTKDNTLQLH
                     YSNWRARTAFAEIDAEILAERMMLKWTKRPGIHLLMTLHGGEEALLADTLDSLATQLY
                     PDWLLTVVTDLPQPEGLDESANLQWLSLQDAVHINYVIDEVAAASPGSWLARIDPGLS
                     LEPQALQVFADYINSRPMWHLIYCDEDTREADGSLSQPLFKPDFDLDLFRAQNYIGAF
                     ALINKEAFLVAGRYGEHRGAEIYDLSLRISDHVGPRAIGHISQMLVHLPRESTRAIAP
                     EAEKAAVIDHLERQGVPAKVSDGIVYGTRRIDYLWPENPLVSIVIQTRDREEYLRPLL
                     ESMHELTQYTNYELVIVDNETSDPDALEWLKALPSDPRWHGRMTVIERRGKFNWSAGA
                     NIGAGAASGKYLLFLDNDIHVVQKEWLGRMLGIAQRPEVGIVGPRLAFAETAKIQDGG
                     WILGLNGLAATPWNCEMELTEPGYMGRAVCDQHVGAVSGSALLVRASLFDDLCGFNET
                     DFPQFNGALDLCLRVSERGLQIVWTPYSMLVHYGGVSTHERRKDIASALEDVIAGKVE
                     REGILQRWLPVLASDPGYNKNLSLVETFKPDHIAPIVWDTNFHDRSRILGIPLSGGAG
                     EYRLRAPLRIIARAGLAQTAICEPPAHLSVRVLSPIEIARTAPDSIIFHQPVDDHQTN
                     TLQSLANLLPRVRRIITIDDLFTAVPKKNSFYKFQYKDARPRLRRTLGLADKLVVSTQ
                     PLADFCSSMIEDIRIMPNCLERAIWDGARPPALPRRKPRVGWAGAQQHLGDLEIIYPV
                     VEALSEEVDWIFMGMCPDPLRPFVREFHDFVRDFEAYPAALAKLDLDLAIAPLELNAF
                     NEAKSNLRLLEYGFMGWPVICTDIFPYQDAPVTRLPNEPQRWISAIREHLAEPEAMRA
                     AGKALQEWVLANFILEDHATDWLEAYGP"
     gene            3594..4430
                     /locus_tag="PKN25_07345"
     CDS             3594..4430
                     /locus_tag="PKN25_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV90749.1"
                     /translation="MTQTINTNVASLNAQRNLTSSQSSLGTTLQRLSSGLRVNSAKDD
                     AAGLAIAERMNSQVRGMNVAIRNSSDAISLSQTAEGALGKIGDSLQRMRDLAVQSANG
                     TNTSGDRDNLQLEFRQLQDEILRVTKDTKFNNQAVLAGTNFTFQVGANTGETIGVSAT
                     AVSATNIQSAAMTGASAIAVSGTNADAAVSAIAAIDLAISEVVKQRATFGAAQTRFES
                     VISNLQVASENQAAARGRIMDADFAAETANLSRTQILQQAGTAMLAQANSLPQNVLSL
                     LR"
     gene            4510..4920
                     /locus_tag="PKN25_07350"
     CDS             4510..4920
                     /locus_tag="PKN25_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNV90750.1"
                     /translation="MSIQPINNASMSMQAMTAAPVRDAQAARNQADAPAGGSRAAEAR
                     GEIIQQNISPQDQNPRTPDIGEVQKALEEVEKAVAPMAQSLQFSLDKDSGRTVVKVMD
                     TDTNEVIRQIPSEEVLAISKAVDKLKGLLLKQQA"
     gene            4940..6283
                     /gene="fliD"
                     /locus_tag="PKN25_07355"
     CDS             4940..6283
                     /gene="fliD"
                     /locus_tag="PKN25_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNV90751.1"
                     /translation="MAISAAGVGSGLDVGSILNQLMAVERQPLSRLQEQQKSYQSKLS
                     AFGQLQSAMSKLQDAATALTKSSTFSATTASAGDTKAFTVSSTASAQTGSYNIEVSQL
                     ARAQRTATSATTAPDLSGGGSIDITLGTGTPKTINLAAGGTLQDLRDAINAADAGVSA
                     QIVNNGTVNQLVISSKETGAASAFKLEGFGGLSEFSFDPAAPAGDMVSVQQAKDAKLS
                     IDGLAITRSSNTVSDAIEGVTLTLAKPTESETTMTVARNDETAKKAIDDFAKAYNELN
                     SLIRSQTSYNAETKKAGTLNGDTGVRSIQSQLRGVFSNPISGLSGATMLSDAGISIRT
                     DGSMSVDSTKLASALADPSKKIAELFAGNGTVDGFAKTLETRIKDMLATDGLLTSRTD
                     GINRTIKSFDSRIEAFELRLEKVQARYSAQFTALDAAMSSMSTTSAYLTQQLANL"
     gene            6325..6735
                     /gene="fliS"
                     /locus_tag="PKN25_07360"
     CDS             6325..6735
                     /gene="fliS"
                     /locus_tag="PKN25_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNV90752.1"
                     /translation="MFGGFGNRAAAYAKVSVETGVNTADPHKLILMLFDGALLQVRTA
                     GIAIGNQDIPGKGTAISKAIEIIINGLKVSLDMNAGGELAQRLAALYDYMSERLLYAN
                     LHNSQPALDEVAGLLATLREAWAEIRSQVQPSAA"
     gene            6997..7344
                     /locus_tag="PKN25_07365"
     CDS             6997..7344
                     /locus_tag="PKN25_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HNV90753.1"
                     /translation="MLTLPTLAALLERYEAMAEAARANDWERLSALEREAAALREAAR
                     GNTSGAMDDAAALAKLPPADAAELREGIARLLALDAEIRSHTDPFLSSVRKLLSAGRH
                     ERALRDAYGASSR"
     gene            7388..8659
                     /locus_tag="PKN25_07370"
     CDS             7388..8659
                     /locus_tag="PKN25_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNV90754.1"
                     /translation="MIPSDLAARLRTLTEASFFETEPPVPGLQRAREIQARLPELVPG
                     QRFLATLQRTLPDGSFRAVVAGQQMTLALDGSAKAGDTLELEVTQLTPRTVFARVVGG
                     EAATTAGASAQPALSQTGRLISFLLTGQPVPSPASLAGNRPLLDAPPTAGSQLVPVLR
                     QALGQSGLFYESHQVQWVLGKLETAALLREPQGQAGGANTAGGGAGASAGGAAAAAAG
                     GAAASGPGATAAAAAGPATATAGQAAGTTAETASRTTEAASSRSAADTEANRSASTTA
                     RAALDETAAPRPGAIPERLMPVVHQQLDALATQQYVWQGQAWPGQNFEWWIEDPQHEG
                     EHAGDEPENTWNTTLRLTLPRLGGVEARLHLTPAGVALRLVADDRATLQTLDGGAAAL
                     ASALEAANLKLTGLVIEPRAVAPNREVASDA"
     gene            8722..9066
                     /locus_tag="PKN25_07375"
     CDS             8722..9066
                     /locus_tag="PKN25_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNV90755.1"
                     /translation="MPDTRPPLRNEAVALAYTAGEAAPRVVAKGKGVVAREILERARE
                     AGVFVHESPELVALLMQVDLDARIPPQLYVAVAELLAWLYRLEQGAEAGAAPVARDLP
                     ESLRPRPSSAEP"
     gene            9344..10123
                     /locus_tag="PKN25_07380"
     CDS             9344..10123
                     /locus_tag="PKN25_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNV90756.1"
                     /translation="MTIDQEDTRIELIHADDEAKYVLRDAREILAIVRNLLGARALVS
                     ARLAPGNESVLSTLVAVAEDGTSLLLDGSADPLQNQRMERADALDCVTQLDKVRIQFL
                     LRGQRLVEDGGSPGFRAPLPEALLRLQRREFYRLQTPISEGVSCTLALPRVDGSRREA
                     ELRILDISGGGVAIAVPPVGVHFEPGTEFPDSLLRLPDTAPISARLVVRNLFRITRRG
                     GAEVLRAGCEFADLPRGAEDAIQRYILRAERTRNARERGRL"
     gene            10177..10968
                     /locus_tag="PKN25_07385"
     CDS             10177..10968
                     /locus_tag="PKN25_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNV90757.1"
                     /translation="MAELSTPSPASPAPLDGGRGDELEKFTLRGARQILQLLQDLITH
                     RGLITAHTGGGHSFMTAVLKVDEERGRVVLDPSPDPQANRRALAAPRLTCVTQLDGIR
                     IQFPLVGLGEGQDKGRPALFAPLPAEMLRLQRREFYRLQVPLAHELSCLLKAEDLARK
                     PVEVSARVIDIGAGGVAVVVPAGAAEFVIGGTLPACRLALPDGEPIELDLEVRNLNRQ
                     TQRNGTEQLRVGLRFAALPRAADTRIQRYIFKTERALNAKARGGL"
     gene            complement(11147..11470)
                     /gene="fliE"
                     /locus_tag="PKN25_07390"
     CDS             complement(11147..11470)
                     /gene="fliE"
                     /locus_tag="PKN25_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNV90758.1"
                     /translation="MDTRGIDQMLSELRSVSQAAQGKAAPAADAPAGGVNFADVLNTA
                     LKDVSAAQGEARAMAQNFSAGDPNVNLQDVMVNLQKASMSFQQMVQVRNRLVTAYQDI
                     MNMPV"
     gene            complement(11789..13210)
                     /locus_tag="PKN25_07395"
     CDS             complement(11789..13210)
                     /locus_tag="PKN25_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV90759.1"
                     /translation="MIETLNILVVEDDAALRDAVCFTLEMAHHRVVGVDGGAAALAAI
                     EREHFNLVVTDLRMQPMDGLQVLHEVRARRPQTPVLLMTAYGDVDKAVAAMRGGACDF
                     LMKPFEPEVLLESVRRYAAVQPAGEDTIAEDPHTRGLLALAARVAESDATVMLSGESG
                     TGKEVFARYIHQHSPRAKGPFIAINCAAIPENLLEATLFGYEKGAFTGAQAAQPGKFE
                     QAQGGTILLDEISEMPLGLQAKLLRVLQEREVERVGGKKPVALDIRVLATSNRDMLGE
                     VAAGHFREDLYYRLNVFPIAIPALRERPGDIVPLARHFLARHAARQQRQVRFSPAAEA
                     LLPLYTWPGNVRELENIMHRVLILTQGDEVSAETIRLCLPQWKPEPANSAGGAAGLAA
                     SPGMQGGVAAVQSGSGQAAGAGTAGPAPERPTNMKDLEREHILATLREFGGSRKKTVE
                     KLGISERTLRYKLQQYREEGHDV"
     gene            complement(13207..14367)
                     /locus_tag="PKN25_07400"
     CDS             complement(13207..14367)
                     /locus_tag="PKN25_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV90760.1"
                     /translation="MPAAAPVLDAAQLAEAFALFSRASEELSTAYAALQAQVGQLSER
                     LSVLMGALPAGVLVVDRAGTVVQANRAAESLFGGGLAGVAWAELAARLEDSGTPGEFV
                     LRRDPAAVDGDPRRLALSESELDSGAERILLVHDVTDTHRMRLTAERNERLAAMGEMV
                     AGLAHQLRTPLAAALLYTGNLRQPELDPAQRAMVADRALERLRYLERLIRDMLLFARG
                     DSAGRQRFAVCELLAELAHTLEPVARARQVAFAADCACGAAELVGDRKALGGALTNLL
                     ENGIQACEAGGTLRCEAVLVGAADAGVGAPAVRFTVRDSGRGIAPELQERLFEPFFTT
                     RAEGTGLGLAIARGVARAHGGDITLRSAPGQGAEFTLVVPLAAAPIGAQENP"
     gene            14571..16283
                     /gene="fliF"
                     /locus_tag="PKN25_07405"
     CDS             14571..16283
                     /gene="fliF"
                     /locus_tag="PKN25_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNV90761.1"
                     /translation="MAAAENTADLPPGAAPADAQATTRMQQALQNLAALSTRQKIAGA
                     VAIALAIALVAGSLLWNRAPEYAVLFSNLDERDGGQIIAELQQRNIPYRMSPTGHAIL
                     VPQAQVHETRLRLAADGLPKGSLAGFELMDGQKLGISQFNEQVNYQRALEGELTRTVQ
                     AIDAVASARVHLAMPKQTAFLRDDQRPTASVMVNLRGGRILSPDQVAGIVHLVSSSVP
                     RMHAEGVKIVDQNGKLLTEQADPLLRAGLDATQLEYVRLLEQGFIERIDKILAPLVGK
                     GNYGAQVAADVDFNQVEQTAETYKPNPTPDQAIRSQQTSEAFNPQPGAQGVPGALTNQ
                     PPVPATAPITSPQVAGGGAQGLASGNRSAVLNYELDRNIQHVKQAVGQIKRLSVAVVV
                     NNRTLPGPDGTPTNVPLPDEEIARITNLVREAVGYNADRGDTINVASGAFADDGSGAA
                     PPPWKDPEIVALGKEGLNWLLVLIAILFAYFGVIRPLLRTVVPPKPKEEKKGAAAGEE
                     GAEGEEGEEGVRVTLSGETGEGEETETFEQRLERARTAARNDPKMVANLIKDWMGMNE
                     EARK"
     gene            16280..17281
                     /gene="fliG"
                     /locus_tag="PKN25_07410"
     CDS             16280..17281
                     /gene="fliG"
                     /locus_tag="PKN25_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNV90762.1"
                     /translation="MTTPEEGLEKAALLLMTLGPDAAAGVLRQLGPREVQKLTMKMAS
                     MTPQSRETVEPVLIEVNEHFGKGSMIQSDDDQLREMLTKALGDDRANQLLSRMMSGSE
                     TAGIESLKWMDAATAADLIKGEHPQIIATILVHLEYDQAGEILKHFDDRLRNDVVLRI
                     ATLDGVQPTALKELNDALTRMLSGATTVKKAAMGGVRHAAEILNFVGSGAETAVIDNV
                     REYDPELAQKILDEMFVFENLLDIDDRGIQSILREVQSDSLIIALKGAPPELREKIFK
                     NMSSRAAEMLREDLEARGPVRLSEVEAEQKEILKTVRRLAEEGQVMLGGKGGDDGFV"
     gene            17396..18274
                     /locus_tag="PKN25_07415"
     CDS             17396..18274
                     /locus_tag="PKN25_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HNV90763.1"
                     /translation="MSISRHQAVGAYRRWEPEDFDAPAGKGSAPPPEAFAPADLAGLV
                     QAAPAPSTASPPIAPPAPEPAPSPPAADSVAALPPDFHLPTAEEIERMHDDMRRAGFE
                     EGRAAGHAEGLEAGRAEGYTEGRARAEAEAARLAGLADSLDEALRELDGEIAEQLMAL
                     AIEMARRMVKLTLAEHPEAILETVRSALLQLPQGHAHIQLHPDDLALVREHLGEQLAH
                     AGHRLQEDARLERGECRIDGQGAQVDATLETRWRRVLESIGHERARWHVAEEAEAGDA
                     TGPVADTAPPAPEPPA"
     gene            18271..19665
                     /gene="fliI"
                     /locus_tag="PKN25_07420"
     CDS             18271..19665
                     /gene="fliI"
                     /locus_tag="PKN25_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HNV90764.1"
                     /translation="MNRHQALWRDFLGDGRRLVEGCNPLEVSGRLTRINGLVMEAAGI
                     KLPLGSGCRILVPGGGAVEAEVVGFNGDKLYMMPTDDVFGLAPGARVLPMEVGVPRLV
                     PGKRIGPRRRAADRAKHLPVGDALLGRVVDGAGRPLDGQGPLHTQETRSLQGRAINPL
                     ARAPIAQPLDVGVRAINALLTVGRGQRLGLFAGSGVGKSVLIGMMARYTEAEVIVVGL
                     IGERGREVKEFIDHSLGPEGLARSVVVAAPADTSPLMRMQGAAYATTVAEYFRDQGKQ
                     VLLIMDSLTRYAMAQREIALAIGEPPVTRGYPPSVFARLPALVERAGNGPEGGGSITA
                     FYTVLAEGDDQQDPIADAARAILDGHFVLSRNLADQGHYPAIDIEQSISRAMHNLVGD
                     DHFNVVRQFKQFFSRYQRSRDLIAVGAYQPGGDPLLDMAVKLYPRLEAFLQQGIQEHE
                     TYEGALQKLAQVFG"
BASE COUNT         3467 a         7016 c         6508 g         2822 t
ORIGIN      
        1 tggactgcaa cgtggtggtc cgctacatcc tgaaccgacc tgggttgctg ggtgaaaggc
       61 cgaactacag cccggacgac ctttactggc tccataacgc agaggtagcc gaagccgttc
      121 cccatcatga gggcgtactg cacatgccgg cagtggacac ctcgattttc aacaacgtgg
      181 acaatcccca cgacaacaac cggcaaggcg cgtgcatcta ctttggccgc tacgtcgaag
      241 gagccaaagc ttttcccgaa ctgacggacc ggtgcaccgt aatcaccaag gattttccaa
      301 ccagtcatga ggaactcgct gcactgtttc gacgcagcac ccacgtctac tgcttcgaga
      361 acacgtccat ctcgatggag gctcgtcttt gcggatgccc agtcgtccaa ctgcccagtc
      421 cctatgccga tccggacaaa cccttcgccg acagcctcgg actctcggac gcactggtca
      481 cctccgacga tgcggaaaca ctcgccgaag cccgtcgcct attgccctcg ctcacccaga
      541 agtacagacg tctcgaggca gcgtactggg aagaactgga acgcctgcta gacaagaccc
      601 aagccgtcgc cgaaagccgc cccaagaccc acaagagccc gaccaaggac aataccctcc
      661 agctacatta ttcgaattgg cgcgcccgca cggcattcgc cgaaatcgat gcggagatac
      721 tcgccgaacg catgatgctc aaatggacga aacgtccagg cattcacctg ctcatgaccc
      781 tccatggcgg cgaggaggct ctgctcgccg acacactcga cagtctggcc acgcagctat
      841 acccggactg gctgcttacg gtcgtgaccg acctgcccca acccgaaggc ctggatgagt
      901 cagcgaacct gcagtggctt tctctgcagg acgccgttca tatcaactac gtgatcgacg
      961 aagttgcagc ggcctcgccc ggctcatggc ttgcccggat cgatccgggc ctgagcctgg
     1021 agccccaggc tctgcaagta ttcgccgatt acatcaattc tcggccgatg tggcatctga
     1081 tttattgtga cgaggacaca cgagaggcgg atgggagctt gagccaaccg ctgttcaagc
     1141 cggacttcga tctcgacctt ttcagggcac aaaattatat cggcgcattc gccctgatca
     1201 acaaggaggc attcctggtt gccgggcgct atggggaaca ccgtggcgcc gagatatacg
     1261 acctgagtct tcgcatatca gaccacgttg gaccacgggc tatcggtcat ataagccaga
     1321 tgctcgttca tctcccccgc gagtcgactc gggcaatcgc ccccgaggca gagaaggcgg
     1381 cagtaatcga tcacctggaa cgccaaggag taccggcgaa ggtgagcgac gggatcgttt
     1441 acggcacccg ccgcatcgac tatctctggc ccgaaaatcc gttggtcagc atcgtcatcc
     1501 agaccaggga tcgcgaagaa tatttgcgcc ccctgctcga aagcatgcat gagctcacgc
     1561 aatacaccaa ctacgagctc gtgatcgtcg acaacgaaac cagcgacccc gatgcgcttg
     1621 aatggttgaa ggctctgccg agcgatccgc gctggcatgg ccgcatgacg gtaattgaaa
     1681 gaaggggcaa gttcaactgg tccgcgggag caaacatcgg cgcgggagca gccagtggaa
     1741 aatatctgct gttcctggac aacgatattc acgttgttca gaaggagtgg ctgggcagga
     1801 tgctaggcat cgcgcagcgc ccggaggtcg gtattgtcgg accacgcctt gctttcgccg
     1861 agaccgcgaa gattcaggat ggcggctgga tcctcgggct gaatggcctc gcggcgaccc
     1921 cttggaactg cgaaatggaa ttgactgagc ccgggtacat gggtcgtgca gtctgtgatc
     1981 agcacgtggg ggcggtgagc ggctccgcat tgctcgttcg tgcatccctt ttcgacgact
     2041 tatgcggctt caacgaaacc gattttccgc aattcaacgg agcgcttgac ctctgccttc
     2101 gcgtttcgga aagaggcctc cagatcgttt ggacgcccta ctcgatgctt gtacactacg
     2161 ggggcgtatc gacccacgag cgtcggaaag acatagccag tgcgctcgaa gacgtcattg
     2221 ccggcaaggt cgagcgtgaa ggcattctgc aaagatggct ccccgtgctc gccagcgacc
     2281 ccgggtacaa taagaatctc agcctcgtcg agacattcaa gccggatcac atcgcgccga
     2341 tcgtgtggga caccaatttc cacgatcgct cgcggatcct gggcatccca ttgagcggcg
     2401 gggctggcga gtaccgtctt cgcgcgccct tgcgcatcat cgcgcgtgcc gggttggcgc
     2461 agaccgcgat ctgcgagcct cccgctcatc tgagcgtccg agtcctctca cctatcgaga
     2521 tcgcccggac cgcacctgat tcgatcattt tccatcaacc ggtcgacgac catcagacga
     2581 acacactaca gagtctggcc aatctcctcc ctcgggtacg tcggatcatc acaatcgatg
     2641 acttgttcac cgcggtcccc aagaagaact cgttctacaa gtttcaatac aaggatgccc
     2701 gcccccgcct gcggagaacg ctgggcttgg ccgacaaact ggtcgtcagc acccagccgc
     2761 ttgcagactt ctgctcgagc atgatcgaag acatccgcat catgcccaac tgcctcgaga
     2821 gagcgatctg ggacggagcc cggccgccag cgctcccacg acgcaaaccc cgggtgggat
     2881 gggcgggtgc ccagcagcac cttggtgatc tcgagatcat ctatccggtc gtcgaggccc
     2941 tttcggaaga agtggattgg atcttcatgg ggatgtgccc cgaccctctc cgtcctttcg
     3001 tgcgagagtt tcatgacttc gttcgagact tcgaagccta tcccgcggca ctcgccaagc
     3061 tcgacctgga tctcgcgatc gcgccactcg agctgaatgc cttcaacgag gcgaagagca
     3121 acctgcgact gctcgagtat ggcttcatgg gttggccggt gatctgcacc gacatcttcc
     3181 cttatcagga tgcgccggtg acccgcctgc cgaacgagcc ccagaggtgg atttccgcga
     3241 tccgcgagca cttggccgaa cccgaagcca tgcgagctgc cggcaaggca ctacaggaat
     3301 gggtcctcgc caacttcatc cttgaggatc acgcgaccga ctggctcgag gcctacggtc
     3361 cctgagcctt tgcgcgcccc ggacggcgcg caggatcggc ccacgaccga ccccgcgcgc
     3421 cgaagacatc ggcggatcca gcgcacgaca gtcggaagat cgaaaaagca ctcaagaagc
     3481 agagcaccgt tgccgtaaca gcattcaaga gcacgacaaa cgctccgcac ccgacgaggc
     3541 aaccgacgca acctgcgggt gaatgaccaa cctgatatcc caggagaaaa atcatgaccc
     3601 agacgatcaa caccaacgtc gcctcgctca acgcgcagcg taacctcacc tcctcccaga
     3661 gctcgctggg cacgaccctg cagcgcctct cctccggcct ccgggtgaat agcgccaagg
     3721 acgacgctgc cggtctcgcg atcgccgaac gcatgaactc ccaggtgcgc ggcatgaacg
     3781 tcgcaatccg caactccagc gacgccatct cgctctctca gacggccgaa ggtgcgctgg
     3841 gcaagatcgg tgattccctg caacgcatgc gcgacctcgc tgtccagtcg gcaaacggga
     3901 ccaacacttc cggcgaccgt gacaacctgc aactcgaatt ccggcagctt caggatgaga
     3961 tccttcgtgt gaccaaagac acgaagttca acaaccaggc ggtccttgcc ggcacgaact
     4021 tcaccttcca ggtcggggca aacacgggtg aaaccattgg cgtctccgcg actgcggtct
     4081 cggcgacgaa catccagagc gcagcgatga ccggcgccag tgcgatcgcg gtcagtggca
     4141 caaacgctga cgcagcagtc tcggccatcg cagcgatcga cctcgcaatt tccgaggtgg
     4201 tcaaacaacg cgcaaccttt ggtgcagcgc agactcgctt cgagtccgtg atctcgaacc
     4261 tccaggtcgc ctccgagaac caggctgccg cccgcggccg catcatggac gccgacttcg
     4321 ccgccgaaac cgcgaacctg tcgcgcaccc agatccttca gcaggccggt accgcgatgc
     4381 tggcgcaggc gaactcgctg ccgcagaacg tgctgtccct gctccgctga ttctcggcaa
     4441 gcgtcatgaa ccgatcggcg cggagcgtag aatacgcttc gcgccgttgg cccatcagga
     4501 ggtcgaatca tgtcaatcca acccatcaac aacgcgtcga tgtcgatgca ggcgatgacc
     4561 gccgcgccgg ttcgcgatgc ccaagcggca cgcaaccagg ccgatgcgcc cgccggcggc
     4621 tcccgtgccg cggaagcacg cggagagatc atccagcaga acatcagtcc acaggaccag
     4681 aatccgcgca ctcccgacat cggcgaggtg caaaaggccc tcgaggaggt cgagaaggcg
     4741 gtcgcgccca tggcgcagag cctgcagttc tcgctcgaca aggattccgg caggaccgtg
     4801 gtcaaggtca tggacaccga caccaacgag gtcatccgcc agattccgtc cgaagaagtg
     4861 ctcgccatct cgaaggcggt cgataagctg aagggtctgc tcctcaagca gcaggcctga
     4921 gcggcaggag ggtagcaaca tggcaatcag cgcagcaggg gtcggctcgg ggctggacgt
     4981 cggcagcatc ctcaaccagt tgatggcggt cgaacgccag ccgctgagtc gcctgcagga
     5041 gcagcagaag agctatcaat ccaagctctc ggccttcggg caactgcaga gcgccatgtc
     5101 caagctgcag gacgccgcaa cggcgctgac caagtcgagc accttctcgg ccacgacggc
     5161 gagcgcgggc gacaccaagg cgttcacggt cagctcgacc gcctcggcgc agacgggcag
     5221 ctacaacatc gaagtcagcc agctcgcccg cgcgcagcgc actgccacga gcgccacaac
     5281 cgcacccgat ctcagtggtg gtggctcgat cgacattacc ctcggcacag gcacgccgaa
     5341 gacgatcaac ctcgcggccg ggggtacgct gcaggacctg cgcgatgcga tcaatgccgc
     5401 cgatgccggg gtcagtgctc aaatcgtcaa taacggcacg gtcaatcagc tcgtcatcag
     5461 cagcaaggaa accggggcag ccagcgcctt caagctcgag ggcttcggcg gattgagcga
     5521 attcagcttc gaccccgcgg cacctgctgg cgacatggtc tcggtccagc aggccaagga
     5581 cgcgaagctc agcatcgacg gtcttgcgat cactcgttcc agcaacacgg tgagcgatgc
     5641 gatcgagggc gtgacgctaa cactcgccaa gccgaccgaa agcgaaacca ccatgaccgt
     5701 ggcccgcaac gacgagacgg ccaagaaggc gatcgacgat ttcgccaagg cgtacaacga
     5761 gctcaattcg ctgatccgca gtcagaccag ctacaacgcc gagaccaaga aggccggcac
     5821 cttgaacggc gacaccggcg tacggtcgat ccagagccag cttcgcggcg tcttctcgaa
     5881 tccgatctcg ggcctgagtg gcgcgaccat gctgtccgat gccgggatca gcatcaggac
     5941 ggacgggagc atgtcggtcg acagcaccaa gcttgcttca gcccttgccg acccctcgaa
     6001 gaagatcgct gagctctttg ccggcaacgg caccgtggat ggctttgcca agaccttgga
     6061 aacccgcatc aaggacatgc ttgcgacgga tggcctgctg acgtcacgaa ccgacggcat
     6121 caatcgcacg atcaagtcct tcgattcccg cattgaagca ttcgagttgc ggctcgaaaa
     6181 ggtgcaggcg cgctacagcg cgcagttcac cgcactcgat gccgccatgt cgagcatgag
     6241 cacaaccagc gcttacctga cccagcaact cgccaacctc taagccgcac acttcggacg
     6301 agcgtttcca ggagagaaga aaaaatgttt ggaggtttcg gtaaccgcgc cgccgcctac
     6361 gcaaaggtca gcgtcgaaac cggcgtcaat accgccgacc cgcacaagct gatcctgatg
     6421 ctgttcgacg gcgccctgct gcaggtgcgc accgccggca tcgcgatcgg caaccaggac
     6481 attcccggca agggcacggc gatctccaag gcgatcgaga tcatcatcaa cggcctcaag
     6541 gtcagcctcg acatgaatgc cggcggcgag ctcgcgcagc gcctcgccgc gctctacgac
     6601 tacatgagcg agcgcctgct ctacgccaac ctgcacaaca gccagcccgc gctcgacgaa
     6661 gtggcgggtt tgctggcgac gctgcgcgag gcctgggcgg agatccgcag ccaggtgcag
     6721 ccgagcgcgg cgtgaggctg cggccgggcg catttcttgc atcggcattt gttgcttctt
     6781 gcttttcgta ggagcgacgc aagtcgcgaa gcaaccgcaa tcccgatcgc cgctgcccgt
     6841 gcgatagacg agccgggcga aggatcgccg ccggtggtgg tggtgggcga ggagtgcatt
     6901 tcgtgacggg ggtgatcgcg acttgtgtcg ctcctacagg tgcgtgctgc gctgttgtcg
     6961 ataatcgaac cgcttcccga tgagtcctct tccgccatgc tgaccctgcc caccctcgcc
     7021 gccctgctcg agcgctacga agcgatggcc gaggcggcgc gtgcgaacga ctgggagcgc
     7081 ctttcggcgc tcgaacgcga ggccgcggcg ctgcgcgagg cggcgcgagg gaatacgtcg
     7141 ggcgcaatgg acgacgcagc cgcgctggcc aagctgccgc cggctgacgc cgccgagctg
     7201 cgcgagggca tcgcgcgcct gctggcgctc gacgccgaga tccgcagcca caccgatccc
     7261 ttcctctcca gcgtgcgcaa gctgctctcc gccgggcgcc acgagcgcgc gctgcgcgac
     7321 gcctacggcg cgtcctcgcg ctgaggccga cgcgccctga ccggccgggc ccgatggagc
     7381 gcatgcgatg atcccctccg acctcgccgc ccgcctgcgc acgctcaccg aagcgagctt
     7441 cttcgagacc gagccgccgg tccccggcct gcagcgcgca cgcgagatcc aggcgcgcct
     7501 gcccgagctc gtgcccggcc agcgcttcct ggccaccctg cagcgcaccc tgcccgacgg
     7561 cagcttccgc gccgtggtgg ccgggcagca gatgacgctg gcgctcgacg gctcggccaa
     7621 ggccggcgac acgctcgagc tcgaggtcac ccagctcacc ccgcgcacgg tgttcgcgcg
     7681 cgtggtgggc ggcgaggcgg ccacgaccgc cggcgcctcg gcgcaaccgg cgctgagcca
     7741 gaccgggcgc ctgatcagct ttctgctcac cggccagccg gtgccctcgc cggcaagcct
     7801 tgccggcaac cgcccgctgc tcgacgctcc tcccaccgcc ggcagccagc tcgtgccggt
     7861 gctgcgccag gcgctcgggc agagcgggct gttctacgaa tcgcaccagg tgcagtgggt
     7921 gctcggcaag ctcgaaaccg ccgcgctgct gcgcgagccg caggggcagg cgggcggggc
     7981 gaacacggcg ggaggcggag cgggtgcgag cgcgggaggc gcggcggcgg ctgcagcagg
     8041 cggggctgct gcgagcggac ccggcgcgac agctgccgcc gctgcgggcc cggccaccgc
     8101 gaccgcaggc caggccgccg gcaccacggc cgaaacggcc tcgcgcacga cggaagccgc
     8161 ctcaagccgc agcgccgccg acaccgaggc caaccgcagc gcctccacca ccgcacgtgc
     8221 cgccctcgac gagaccgcag ccccccgccc cggcgccatt cccgagcgcc tgatgccggt
     8281 ggtgcaccag cagctcgacg cgctcgccac ccagcaatat gtctggcagg gccaggcctg
     8341 gccggggcag aacttcgagt ggtggatcga ggatccgcag catgagggcg agcacgccgg
     8401 cgacgagccc gagaacacct ggaacacgac gctgcgcctc accctgccac ggctcggcgg
     8461 cgtcgaagcc cggctccacc tcacccccgc cggtgtcgcc ctgcgcctgg tcgccgacga
     8521 ccgcgccacc ctgcagaccc tggacggcgg cgccgccgcg ctcgcctcgg ccctcgaagc
     8581 cgccaacctc aagctcaccg ggctggtgat cgagccgcgc gcagtggcgc cgaataggga
     8641 ggtggcaagc gatgcctgac aagtacatcc tgcgacgcaa ccagaccgcc gcctgtacca
     8701 gcgggcaccg agccgccgcc catgcctgac acccgcccac cgctgcgcaa cgaggccgtc
     8761 gccctcgcct acaccgccgg cgaggccgcg ccgcgcgtgg tcgcaaaggg caagggcgtg
     8821 gtggcgcgcg agatcctcga gcgcgcgcgc gaggccgggg tgttcgtgca tgagtcgccg
     8881 gagctggtcg cgctgctgat gcaggtcgac ctcgacgccc gcatcccgcc ccagctctac
     8941 gtcgcggtcg ccgagctgct cgcctggctc taccgcctcg aacaaggcgc cgaagccggt
     9001 gccgcgccgg tcgcccgcga tctgccggag tcgctgcgcc cgcgcccgtc ttctgccgaa
     9061 ccatgagact gccgcgccga tcgcgacttg cgtcgctcct acatcccacc ccaggcaccg
     9121 cgggcctgcc gtaggagcga cgccagtcgc gattctgccg gcaaggacag gcgccgttcg
     9181 acttccacgc cccgatgatc gcgacttgca tcgcacctgc ggggcctccc ggacgccgcg
     9241 gcctcgcggg gcgcgctacc acgcccccgg ggcgtgtctc gtgatataag aaaacgagtt
     9301 tgtgcagcag ccgtgcgctg cctcaagcga cgagccccaa ggcatgacga tcgaccaaga
     9361 agacacccgc atcgaactca tccacgccga cgacgaggcg aagtacgtgc tgcgcgacgc
     9421 gcgcgagatc ctcgccatcg tgcgcaacct gctcggtgcg cgggcgctcg tgagcgcacg
     9481 gctggcgccg ggcaacgaat ccgtgctgtc caccctggtc gcggtcgcgg aagacggcac
     9541 cagcctgctg ctcgacggca gcgccgaccc gctgcagaac cagcgcatgg agcgcgccga
     9601 cgcgctcgac tgcgtgaccc agctcgacaa ggtgcgtatc cagttcctgc tgcgcggcca
     9661 gcgcctcgtc gaggacggcg gcagcccggg cttccgcgcg ccgctgccgg aagccctgct
     9721 gcgcctgcag cgacgcgagt tctaccgcct gcagacgccg atctcggagg gggtgagctg
     9781 cacgctggcc ctcccccgtg tcgacggcag ccggcgcgag gccgagctgc gcatcctcga
     9841 catcagcggc ggcggcgtgg cgatcgcggt gccgccggtg ggcgtgcact tcgagcccgg
     9901 aaccgagttc cccgacagcc tgctgcgcct gcccgacacc gcaccgattt ccgcgcgcct
     9961 ggtggtgcgc aacctcttcc gcatcacccg gcgcggcggc gccgaggtgc tgcgtgccgg
    10021 ctgcgagttc gccgacctgc cgcgcggcgc cgaggacgcg atccagcgct acatcctgcg
    10081 cgccgagcgc acccgcaacg cgcgcgagcg cggccgcttg tgaacccgcg gcaccccacc
    10141 cgcctcccga ccctgcccaa gacacgatag ccccgcatgg ccgaactcag caccccaagc
    10201 cccgccagcc cggcaccgct cgacggcgga cgcggcgacg agctcgagaa attcaccctg
    10261 cgcggcgcgc gccagatcct gcagctgctg caggatctca tcacccaccg cggcttgatc
    10321 accgcccaca ccggcggcgg ccactccttc atgaccgcgg tgctgaaggt ggacgaagag
    10381 cgcgggcgcg tggtgctcga ccccagcccc gacccccagg ccaaccgccg tgcgctcgcc
    10441 gcgccgcgcc tgacctgcgt gacccagctc gacggcatcc gcatccagtt cccgctcgtc
    10501 ggcctcggcg aaggccagga caagggccgc cccgccctct tcgcacccct gcccgccgag
    10561 atgctgcgcc tgcagcggcg cgagttctac cgcctgcagg taccgctcgc gcacgagctg
    10621 agctgcctgt tgaaagcgga ggacctcgcc cgcaagccgg tggaggtgag cgcacgcgtg
    10681 atcgacatcg gtgccggagg ggtggcggtg gtggtgcccg cgggggcggc ggaattcgtc
    10741 atcggcggca cactgccggc ctgccgcctc gcgctgccgg acggcgagcc gatcgaactc
    10801 gacctcgagg tgagaaacct caaccgccag acgcagcgca acggcaccga acaactgcgg
    10861 gtgggcctgc gcttcgccgc cctgccgcgc gctgcggaca cccgcatcca gcgctacatc
    10921 ttcaagacgg aacgcgcgct caacgccaag gcacgcggag gcctgtaggc ttgatgccaa
    10981 gcctttccgc agcgccccgg tcgcgacttg cgtcacctgc atcccacccg agacgccggg
    11041 ggtcttcgta ggagcgacgc aagtcgcgat tccggcgctc gaaatgcgcg acaacgcccc
    11101 ggtcgcgact tgcgtcgctc ctacgggaga accgcggcga agccggtcag accggcatgt
    11161 tcatgatgtc ctgatacgcc gtgaccaagc ggttgcggac ctggaccatc tgctggaagg
    11221 acatgctggc cttttgcagg ttgaccatga cgtcctgcag attgacgttg gggtcgccgg
    11281 cggagaagtt ttgcgccatc gcgcgcgcct cgccctgggc ggcgctgacg tctttcaagg
    11341 cggtgttgag cacgtcggcg aagttgacgc ctcccgcggg cgcgtccgcg gcgggtgcgg
    11401 ccttgccttg cgcggcctgc gacaccgagc gcagttcgct cagcatctgg tcgattccac
    11461 gggtgtccat agcgtaatcc cccggcttca gggtggtcgg tgacgtcagt gtagcagagc
    11521 aaatcgcgtg ccagagccgc cggggacgcg cggtcgttgc ggttttcgta ggagcgacgc
    11581 aagtcgcgac gacggcgtcg gcactgattc catgcgcccg atcgcgactt gcgtcgctcc
    11641 cacaggcgcc ttcggaagag ggttgacttg ccgtaggagc gacgcaagtc gcgagcgggc
    11701 gatcgtgcaa tccggtggcc gatgggcggc cggaaagggc tgcggggccg ggatcgcgac
    11761 tcgcgtcgct cccacagggc gcccggtctc acacgtcgtg gccctcctcg cggtattgct
    11821 gcagcttgta gcgcagggtg cgctcggaga tgccgagctt ttccacggtc ttcttgcgcg
    11881 agccgccgaa ctcgcgcagc gtggcgagga tgtgctcgcg ctcgaggtct ttcatgttgg
    11941 tcgggcgctc gggagccgga ccggccgtgc cggccccggc ggcctgcccg ctacccgact
    12001 gcacggccgc gacccccccc tgcatcccgg gagaagcggc aagccctgcc gccccgccgg
    12061 cagaatttgc cggctccggc ttccattgtg gcaagcacag gcggatcgtc tcggcgctca
    12121 cctcgtcgcc ctgggtcagg atcagcacgc ggtgcatgat gttttccagc tcgcgcacgt
    12181 tacccggcca ggtgtacagc ggcagcagcg cctcggcggc gggcgagaag cgcacctggc
    12241 gctgctggcg ggcggcgtgg cgggcgagga agtggcgcgc cagcggcacg atgtcgcccg
    12301 ggcgctcgcg cagcgcgggg atggcgatcg ggaacacgtt gaggcggtaa tacagatcct
    12361 cgcggaagtg cccggcggcg acctcgccga gcatgtcgcg gttgctggtg gcgagcacgc
    12421 ggatgtcgag ggccaccggc ttcttgccgc cgacgcgctc gacctcgcgc tcctgcagca
    12481 cgcgcagcag cttggcctgc aggccgagcg gcatctcgga gatctcgtcg agcaggatgg
    12541 tgccgccctg ggcctgctcg aacttgcccg gctgcgccgc ctgcgcaccg gtgaaggcgc
    12601 ccttctcgta gccgaagagc gtggcctcga gcaggttctc ggggatggcc gcacagttga
    12661 tcgcgatgaa ggggcccttg gcgcgcggcg aatgctggtg gatgtaacgc gcgaagacct
    12721 ccttgccggt gccggactcg cccgagagca tcaccgtcgc atccgactcg gccacgcgcg
    12781 cggcgagcgc gagcaggccg cgggtgtggg ggtcctcggc gatggtgtcc tcgcccgcgg
    12841 gctgcaccgc ggcgtagcgg cgcacgctct cgagcagcac ctcgggctcg aagggcttca
    12901 tcaggaagtc gcaggcgccg ccgcgcatcg ccgccaccgc cttgtcgacg tcgccatagg
    12961 cggtcatcag cagcaccggc gtctgcggcc ggcgcgcgcg cacctcgtgc agcacctgca
    13021 ggccgtccat gggctgcatg cgcaggtcgg tgacgaccag gttgaagtgc tcgcgctcga
    13081 tcgccgcgag cgccgccgcg ccgccatcga cgccgacgac gcggtggtgc gccatctcca
    13141 gcgtgaaaca gaccgcgtcg cgcagcgcgg cgtcgtcctc gacgacgagg atgttgaggg
    13201 tctcgatcat gggttctctt gtgcgccaat gggcgccgcc gccagcggca cgacgagggt
    13261 gaattccgcg ccctgcccgg gcgcggagcg cagggtgatg tcgccgccat gggcgcgcgc
    13321 gacgccgcgg gcgatcgcca ggcccagccc ggtgccttcg gcgcgggtgg tgaagaaggg
    13381 ctcgaagagg cgctcctgca gctcgggggc gatgccgcgg ccgctgtcgc gcaccgtgaa
    13441 gcgcaccgcg ggcgcgccca cgccggcgtc ggcggcgccg accagcacgg cctcgcagcg
    13501 cagcgtgccg cccgcctcgc aggcctggat gccgttctcc agcaggttgg tgagcgcgcc
    13561 gccgagcgcc ttgcggtcgc cgaccagttc ggccgcgccg caggcgcagt cggccgcgaa
    13621 cgccacctgg cgcgcgcgcg ccaccggttc cagcgtgtgc gcgagctcgg cgagcagctc
    13681 gcacaccgcg aagcgctgac gtccggcgct gtcgccgcgc gcgaacagca gcatgtcgcg
    13741 gatgaggcgc tcgaggtagc gcaggcgttc gagcgcgcgg tcggccacca tggcgcgctg
    13801 cgccgggtcg agctcgggct ggcgcaggtt gccggtgtat agcagcgcgg cggcgagcgg
    13861 cgtgcgcaac tggtgggcga gcccggcgac catctcgccc atcgccgcaa ggcgctcgtt
    13921 gcgctcggcg gtcaggcgca tgcgatgggt gtcggtgacg tcgtgaacga gcaggatgcg
    13981 ctcggcaccg gagtcgagct cggactcgga cagggccaag cggcgcgggt cgccatcgac
    14041 cgcggcgggg tcgcggcgca gcacgaattc gcccggggtg ccgctatctt cgaggcgggc
    14101 ggcgagctcc gcccacgcca ccccggcaag cccgcctccg aacaagctct cggcagcgcg
    14161 gttggcctgc accacggtgc cggcacgatc gaccaccagc accccggcgg gcagcgcccc
    14221 catcagcacc gacaggcgtt cgctgagctg gccgacctgg gcctgcagcg cggcgtaggc
    14281 ggtggagagc tcctcggagg cgcggctgaa gagcgcgaag gcctcggcga gctgggcggc
    14341 gtcgagcacc ggcgccgcgg cgggcatgct ccctgcttga tcggaggcga cgactgcggc
    14401 ccgcatggga aggtccgaat ccgttttcac gggatgcact ctaaatgcac aggccgccat
    14461 ggtagccggc aaacaggcgg caaaaaatgc cgcttttcac gccacgaggc ttgcacgcgc
    14521 ggacgcacaa tgccgccatc cgccaaagcg tatgcaacag gaccgccatc atggccgcag
    14581 cagaaaacac cgccgacctg cctccgggcg ccgcccccgc cgatgcgcag gcgacgacgc
    14641 ggatgcagca ggcgctgcag aacctcgccg cgctcagcac gcgccagaag atcgccggcg
    14701 cggtcgccat cgcgctggcg atcgcgctcg tggccggctc cctgctgtgg aaccgcgccc
    14761 cggagtacgc ggtgctgttc tccaacctcg acgagcgcga cggcggacag atcatcgccg
    14821 agctgcagca gcgcaacatc ccctacagga tgtcgcccac cggccacgcc atcctggtgc
    14881 cgcaggcgca ggtgcatgag acgcggctgc gtctggcggc cgacggcctg cccaagggca
    14941 gcctcgccgg cttcgagctg atggacggcc agaagctcgg catctcgcag ttcaacgagc
    15001 aggtgaacta ccagcgcgca ctcgaaggcg agctgacccg caccgtgcag gcgatcgacg
    15061 cggtggccag cgcccgggtg cacctggcga tgcccaagca gaccgccttc ctgcgcgacg
    15121 accagcgccc caccgcctcg gtgatggtga acctgcgcgg cggccgcatc ctctcgcccg
    15181 accaggtcgc cggcatcgtg cacctggtgt cgtcgagcgt gccacgcatg cacgccgagg
    15241 gcgtgaagat cgtggaccag aacggcaagc tgctcaccga gcaggccgac ccgctgctgc
    15301 gcgccgggct cgacgccacc cagctcgaat acgtccgcct gctcgagcag ggcttcatcg
    15361 agcgcatcga caagatcctc gcgcccctgg tgggcaaggg caactacggc gcccaggtgg
    15421 cggccgacgt ggacttcaac caggtcgagc agaccgccga gacctacaag cccaacccca
    15481 cacccgacca ggcgatccgc agccagcaga ccagcgaggc cttcaacccg cagccgggcg
    15541 cgcagggcgt gcccggcgcg ctcaccaacc agcccccggt gccggccacc gcaccgatca
    15601 ccagcccgca ggtggccggc ggcggcgcgc agggcctggc gagcggcaac cgcagcgcgg
    15661 tgctcaacta cgagctcgac cgcaacatcc agcacgtcaa gcaggcggtc gggcagatca
    15721 agcgcctgtc ggtcgcggtg gtggtgaata atcgcaccct ccccgggccc gacggcacgc
    15781 ccaccaacgt gccgctgccc gacgaggaga tcgcgcgcat caccaacctg gtgcgcgagg
    15841 cggtgggcta caacgccgac cgcggcgaca ccatcaacgt cgccagcggc gccttcgccg
    15901 acgacggcag cggagccgca ccgccgccgt ggaaggatcc ggagatcgtc gcgctcggca
    15961 aggaaggcct gaactggctg ctggttctga tcgcgatcct gttcgcctac tttggcgtga
    16021 tccgcccgct gctgcgcacc gtggtgccgc ccaagccgaa ggaagagaag aagggcgccg
    16081 ccgcgggcga ggaaggcgcg gaaggcgaag agggcgagga aggcgtgcgc gtcaccctgt
    16141 ccggcgagac cggcgagggt gaagagacgg agaccttcga gcagcgcctc gagcgtgccc
    16201 gcaccgcggc gcgcaacgat cccaagatgg tcgccaacct gatcaaggac tggatgggca
    16261 tgaacgagga ggcgcgcaag tgacgacacc cgaggaaggc ctggagaagg cggcgctgct
    16321 gctgatgacc ctcggccccg acgccgccgc cggggtgctg cgccagctcg gcccgcgcga
    16381 ggtgcagaag ctgaccatga agatggcctc gatgacgccg cagtcgcgcg agaccgtgga
    16441 gccggtgctg atcgaggtga acgagcactt cggcaagggc tcgatgatcc agtccgacga
    16501 cgaccagctg cgcgagatgc tcaccaaggc gctcggcgac gaccgcgcca accagctgct
    16561 gtcgcgcatg atgagcggct cggagaccgc cggcatcgag agcctgaagt ggatggacgc
    16621 ggccaccgcc gccgacctga tcaagggcga gcacccgcag atcatcgcca ccatcctggt
    16681 gcacctcgag tacgaccagg ccggcgagat cctcaagcat ttcgacgacc gcctgcgcaa
    16741 cgacgtggtg ctgcgcatcg ccacgctcga cggcgtgcag ccgaccgcgc tgaaggagct
    16801 caacgacgcc ctcacgcgca tgctctcggg cgcgaccacg gtgaagaagg cggccatggg
    16861 cggggtacgc cacgccgccg agatcctcaa cttcgtcggt tcgggcgccg agaccgcggt
    16921 catcgacaac gtgcgcgaat acgacccgga gctggcgcag aagatcctcg acgagatgtt
    16981 cgtgttcgag aacctgctcg acatcgacga ccgcggcatc cagtccatcc tgcgcgaggt
    17041 gcagtccgac tcgctgatca tcgcgctcaa gggcgccccg cccgagctgc gcgagaagat
    17101 cttcaagaac atgtcctcgc gcgccgccga gatgctgcgc gaggacctcg aggcgcgcgg
    17161 cccggtgcga ctgtccgagg tcgaggccga gcagaaggag atcctcaaga cggtgcgccg
    17221 cctggccgag gaaggccagg tcatgctcgg cggcaagggc ggggacgatg gcttcgtgtg
    17281 atccccgcgc cgcccgcatg cggagttcgc ccgcggcgca cccgtgcggc cgcgcgggag
    17341 gaagcgcgtg gcgcggccca gggtggagcc cgcgtggcga caggaggatc gtgccgtgag
    17401 catctcgcgc caccaggcgg tcggcgccta ccggcgctgg gaaccggagg acttcgatgc
    17461 ccccgccggc aagggctcgg caccgccgcc ggaagccttc gcgcccgcgg atctcgcagg
    17521 cctcgtgcaa gcggcgcccg cgccctccac agcctcgccg cccattgccc cccccgcgcc
    17581 ggagcccgcc ccgtccccgc ccgcagccga ctccgtcgcc gcgctgccgc ccgatttcca
    17641 cctgcccacc gcggaggaga tcgagcgcat gcacgacgac atgcgccgcg ccggcttcga
    17701 ggaaggccgc gcggccggcc acgccgaggg gctggaagcc gggcgtgccg aaggctacac
    17761 cgagggtcgt gcacgggccg aggccgaggc tgcgcgcctg gccgggctcg ccgacagcct
    17821 ggacgaggcc ttgcgcgagc tcgacggcga gatcgccgag cagctgatgg cgctggcgat
    17881 cgagatggcg cggcggatgg tgaagctcac ccttgccgag caccccgagg ccatcctcga
    17941 gacggtgcgc agcgcgctgc tccagctgcc tcagggccac gcccacatcc agctccaccc
    18001 cgacgacctc gccctggtgc gcgagcacct tggcgaacag ctcgcccacg ccggccaccg
    18061 cctgcaggag gacgcccgcc tcgaacgcgg cgagtgccgc atcgacggcc agggcgcgca
    18121 ggtggatgcg acgctggaga cgcgctggcg gcgggtgctc gaatccatcg gccacgagcg
    18181 cgcgcgctgg cacgttgcgg aggaagcgga ggcgggcgac gcgaccggcc cggttgccga
    18241 caccgccccg cccgcgccgg agccgccggc atgaaccgtc accaggcgct gtggcgcgac
    18301 ttcctcggcg acggccgccg cctggtcgag ggctgcaacc cgctcgaggt ctccggccgc
    18361 ctcacccgca tcaacggcct ggtcatggaa gcggccggca tcaagctgcc gctcgggtcg
    18421 ggctgccgca tcctggtgcc cggcggcggc gcggtcgagg ccgaggtggt cggcttcaac
    18481 ggcgacaagc tctacatgat gcccaccgac gacgtcttcg gcctggcgcc cggcgcgcgc
    18541 gtgctgccga tggaagtcgg tgtgccgcgc ctggtcccgg gcaagcgcat cggcccgcgc
    18601 cggcgcgccg ccgaccgcgc caagcacctg ccggtgggcg acgccctgct cggccgcgtg
    18661 gtcgacggcg ccggccgccc gctcgacggc cagggcccgc tgcacaccca ggagacgcgc
    18721 tcgctgcagg ggcgtgccat caacccgctc gcacgcgcgc cgatcgcaca gccgctcgac
    18781 gtcggcgtgc gcgcgatcaa cgcgctgctc accgtcggcc gcggccagcg cctcggcctg
    18841 ttcgccggtt ccggcgtggg caagtcggtg ctgatcggca tgatggcgcg ctacaccgag
    18901 gccgaggtca tcgtcgtcgg gctgatcggc gagcgcggcc gcgaggtcaa ggagttcatc
    18961 gaccacagcc tgggccccga gggcctggcg cgctcggtgg tggtggcggc gcccgccgac
    19021 accagcccgc tgatgcgcat gcagggcgcg gcctatgcca ccacggtggc ggagtacttc
    19081 cgcgaccagg gcaagcaggt gctgctgatc atggactcgc tgacgcgcta tgcgatggcg
    19141 cagcgcgaga tcgcgctcgc gatcggcgaa ccgccggtca cccgcggcta cccgccgagc
    19201 gtgttcgccc gcctgcccgc gctggtcgag cgcgccggca acggccccga gggcggtggc
    19261 tcgatcaccg ccttctacac cgtgctcgcc gagggcgacg accagcagga cccgatcgcc
    19321 gacgcggcgc gcgccatcct cgacggccac ttcgtgctct cgcgcaatct cgccgaccag
    19381 ggccactacc cggcgatcga catcgagcag tcgatctcgc gcgcgatgca caacctcgtc
    19441 ggcgacgacc acttcaacgt ggtgcgccag ttcaagcagt tcttctcgcg ctaccagcgt
    19501 tctcgcgacc tgatcgccgt cggcgcctac cagcccggcg gcgatcccct gctcgacatg
    19561 gccgtcaagc tctaccccag gctcgaagcc ttcctgcaac aaggcatcca ggaacacgaa
    19621 acctacgagg gtgccctgca gaagctggcg caggtgttcg ggtgagaaag gcacgaggcg
    19681 caagcgctcc cgctcgcccg taggagcgaa gccagccgcg atcgggcggc gaagctgcgc
    19741 cgccgcgggg ccgaagcgcc gcgatcgcga ctggcgtcgc tcctgcaccc gcgacatcga
    19801 tcgcgagcat ttc
//
LOCUS       DAOQBS010000049        19772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1887_length_19772_cov_12.229295, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000049 DAOQBS010000000
VERSION     DAOQBS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 19772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19772
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1887_length_19772_cov_12.229295"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="PKN25_07425"
     CDS             complement(<1..112)
                     /locus_tag="PKN25_07425"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNV90765.1"
                     /translation="MSERCAWCGTDPLYVAYHDTEWGVPNHDDRHLFEMLV"
     gene            complement(230..1558)
                     /locus_tag="PKN25_07430"
     CDS             complement(230..1558)
                     /locus_tag="PKN25_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNV90766.1"
                     /translation="MKSRSSCLALCFVLAALTLFVALPVRAGALVIATVDSAHMRRLQ
                     TLSSEFEKANPEIRIRWVVLEESELRRVASADVETRDGLFDVITIGIFKVPIWAASGW
                     IKPLRSPDDFDVDDLLPTVRKALSYQQELYAAPIYGESSMLMYRKDLLKKAGLTMPDR
                     PTWTEVAAFAAKLHDPANGVHGICLRGRPGWIENMALVSTMVNSFGGQWFDAGWRPQL
                     DSLPWKKAVGLYAELLNRYGPPDAASLGYGENLSLFASGRCAQWVGPTVAAEVLTNPE
                     SSAVAGEVGFAAAPYEVTAKGAQWLWAWALAIPSSIDPVREADARKFIHWVASRDYVE
                     LVAARQGWRAVPTGTRRSTYARPEFQRKAPWAVHELEAMLDADPEDATLPPSPYPGIQ
                     FAAIPEFILIGDEVGNLIAEALAGRLSVEEALVRGQDAAQRRMVRGGYPR"
     gene            complement(1558..4572)
                     /locus_tag="PKN25_07435"
     CDS             complement(1558..4572)
                     /locus_tag="PKN25_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV90767.1"
                     /translation="MLVLEEVMISVRMLLLGRSLSRLLAVALATLSFGVQADGGKLRV
                     VTDNNYPPYVFPGPDGSPQGYVVDLWKLWEEKTGASVALQAMQWADAQRAILDGRADV
                     IDMIFRTPVREQLYDFSQPYATLPVSIFVDPSINGIVDAGSMQGFAVAVQRGDACIDR
                     LTTLGVTNLVPYASYEAVLAAARDGDIKMFCMDEKPANYYLYLYRDQVKFTRAFTLYE
                     GQFHWAVAKGDAGTFARVQEGMARITPAELEALSKKWLAKPLEFRSYLRIILMVAGIA
                     LAVLAVAMLWIRFLRGLVYARTAEIHQKNEALEQAARELVVEKAQLRAIFDSSPDGMA
                     LKDRNGVYVDCNAVLARGFGLARAQIIGRSDDQLFEDKDFVATVRKNDAEVLGQGASH
                     RYERRFEAADGTAYELEIVKVPVRLAADGAIDGVLVVAHDLTARRRAESELRIAAAAF
                     ESQDGMMITNGRGVIERINSAFTRMSGYPAEEVVGCTPRFLKSDVHDPDFYSAMWGAV
                     ARKGYWAGEIVNRHRDGGLFITRLSITAVCDTQGRPLHHVCSYQDISAERQARELAER
                     LKLYDQLTNLPNRSLLEDRITHALANSAERGELGAVMMLDLDLFQKVNDSWGHGVGDR
                     LLTEMVRRMQGVADNGETLGRFSGDSFVLMLENLGHDRQAAAGRAQDLAEAVRQAIAV
                     PMTIEGRRISSTATIGVTLFRGREASPETLLRQAELAMYKGKNMGRNVVRFFEDAMQS
                     ELDRRNLLEEELREAIENHQLVLHYQVQVDAEGLPIGAEALIRWIHPVRGLVPPFVFI
                     PLAEETGLIEPIGRWVLATACQQLAQWSYDDLFSRLTLAVNVSPPQFKAASFVQDVFD
                     ELERAGVPARTLKLEVTESLAIDDFDASVDKLRQLRARGVRISLDDFGTGNSSLNYLT
                     KLPLSQLKIDKSFVDELPASHRDAMVAQTIIAMGQGLELDVIAEGVETRAQHEYLVAQ
                     GCHAFQGYLFGKPLPVDAFEAAVRERASSQVS"
     gene            complement(4935..5588)
                     /locus_tag="PKN25_07440"
     CDS             complement(4935..5588)
                     /locus_tag="PKN25_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV90768.1"
                     /translation="MLVTQADAFMGPALCDAFRAAGAEVVPDRSALLERGAGRAVIEA
                     AGRIDVLVLNLAIPAPSTPVHQVSDGEWETTFAALVHPMREMVAAVLPQMIERKAGKI
                     LLMGSAAALRGMALRSSYAAARGAQLAYIQAVGVEAAAHGVQVNAIAQNFVENPTYFP
                     PEVQATPAFKDRLKWQVPLGRLVTADEDASFAVYLCSEAANCFVGQVFPVCGGWVNR"
     gene            5844..>6405
                     /locus_tag="PKN25_07445"
     CDS             5844..>6405
                     /locus_tag="PKN25_07445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV90769.1"
                     /translation="MPRIEPFEAHAARYDAWFERHQAAYVSELLALRSFVPLSGRGLE
                     IGVGSGRFAAPLGVQVGIDPSRTMLERARARGVEVCEGTAEHLPFAEHSFDHALVVTT
                     LCFVDSPAAMLTQARRVLKPGGSLVIAFIDRDSPLGRDYEAHRETSDFYRDAHFHSAG
                     EVERLLLDAGFSLRSWAQTLARPLAEN"
     assembly_gap    6406..6505
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6765..7148
                     /locus_tag="PKN25_07450"
     CDS             6765..7148
                     /locus_tag="PKN25_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV90770.1"
                     /translation="MKQVQAIRPDREVLTRQRLPYFIGISGQTVGASGLSMHLVTIPP
                     GARSEPHVHVGYETGIYVLEGRVLTRWGAKLENEVISAAGEFLFVPAGVPHEAINLSA
                     TEAARAVVARNHPAEQDQVQPYVAG"
     gene            7291..8367
                     /locus_tag="PKN25_07455"
     CDS             7291..8367
                     /locus_tag="PKN25_07455"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNV90771.1"
                     /translation="MPTQTENLNVLAFDHMPSPDEVKARVPLTERAAAAVVAGRKAVM
                     DILDRKDPRVFVVVGPCSIHDPVAGLDYARRLKALADEVSDVLLLVMRVYFEKPRTST
                     GWKGYINDPFMDDSFRIDVGMERARKFLLDVCELGLPTATEALDPIAPQYYGDLIAWT
                     AIGARTSESQTHREMASGLSTPVGFKNATDGDLEVAINAIISAGSPHSFLGINSQGQS
                     AVTRTRGNRYGHVVLRGGGGRPNYDTVSVSLAEQALAKAKLAKNIVVDCSHANSWKKP
                     EYQPLVMKDVMHQIREGNQSIVGLMIESNIEAGNQPIPADLSQLKYGCSVTDACVDWA
                     TTEDMIRKSAAVLRDVLPKRERRA"
     gene            8364..9209
                     /gene="serB"
                     /locus_tag="PKN25_07460"
     CDS             8364..9209
                     /gene="serB"
                     /locus_tag="PKN25_07460"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNV90772.1"
                     /translation="MNLVVLAEDVDSVALKNVAKVSEASAIEQITTRSFRLVGAKPVE
                     GLVAACAAGGLDHVWLPEGRQLRDFGLFVTDMDSTLINIECIDEIADMQGFKREVAAI
                     TEAAMQGEIDFRESLTRRVALLAGLPESALAEVFDHRLQLNPGAERLLQGLQRAGIRT
                     MLVSGGFTYFTERLKSRLGFDYAWANQLEVYDGKLTGKVLGDIVDGEAKAAHLEQVRD
                     ELGLRPEQVIAAGDGANDIPMLRAAGFGVAFHAKPVLRDVAHCCLDHTGLDGILDLFG
                     PGGES"
     assembly_gap    9235..9334
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9425..10129)
                     /locus_tag="PKN25_07465"
     CDS             complement(9425..10129)
                     /locus_tag="PKN25_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90773.1"
                     /translation="MKRNGIELSNPVIEVDDLCKQVALADGQGELRILQDIRFAVGAG
                     ESVAIVGASGSGKSTLLGLLAGLDVPAHGTVRVQGRDLFALDEDARAALRGEAIGFVF
                     QSFQLLPALTALENVMLPLELAGVDDARAIATQWLQRVGLGSRLTHYPKHLSGGEQQR
                     VALARAFAPDPRVLLADEPTGNLDAETGRAIIELMFALNREQGTTLILVTHDESLARR
                     CGRVLRMDAGRLSEAP"
     gene            10113..10748
                     /locus_tag="PKN25_07470"
     CDS             10113..10748
                     /locus_tag="PKN25_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNV90774.1"
                     /translation="MPLRFIIPFAFMFLFGAVARADTILVWGDSLSAGYGLERGQEWP
                     TLLQTRLQAEGFRHVVVNASVSGETSSGGLTRLPAALAEHAPTLVILELGANDGLRGL
                     KPQLLADNLAAMIDAAHAAGARVLLIGMRMPPNYGPAYTQRFAEVFANVAAEKKVPLV
                     PFLMDGFADRPELFLPDGIHPAAEAQPRIVDTVWAGLRPLLAAKETKAARK"
     gene            complement(11011..12051)
                     /gene="selD"
                     /locus_tag="PKN25_07475"
     CDS             complement(11011..12051)
                     /gene="selD"
                     /locus_tag="PKN25_07475"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNV90775.1"
                     /translation="MNEIRLTEFSHGGGCGCKIAPAVLAELLATMPAGLVPPELLVGT
                     DTADDAAVYKLNERQAIVATTDFFTPIVDNPRDFGRIAATNALSDVYAMGAQPILALA
                     IVGMPLDKLPPAVIGEILKGGAEVCRDAGIPVAGGHSIDVLEPIYGLVGLGVVDPARV
                     RTNAGAKAGDVLILTKPLGIGILSAGLKKGRLSADGYAQMIRWTTTLNRVGARLADLD
                     GVHAVTDVTGFGLAGHLLEMCRGASLTGAVRFDALPVIEEARALVRDGVATGASTRNW
                     ASYGASVELPVDAPEWQRKLVTDPQTSGGLLIACAPEAVEAVQAAVRAEQGEAGTIVG
                     EMKAGAARVVVA"
     gene            12111..13163
                     /gene="mnmH"
                     /locus_tag="PKN25_07480"
     CDS             12111..13163
                     /gene="mnmH"
                     /locus_tag="PKN25_07480"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNV90776.1"
                     /translation="MQKGVATVAQLSGFDEIIDARTPAEFAEDHIPGAINLPVLDNDE
                     RIVVGTMYKQQSAFDARRLGGALVAANISRHMLATLQDKPKNWRPLIYCWRGGQRSGS
                     FTTWLRMVGWDACQLEGGYKAFRHLVIAEIERIVPTLALRIVCGPTGSAKTRVLEALA
                     AQGAQVLDLEDLAAHKGSVLGALPDRPQPAQKRFETLLWDRLRRLDPARPVYVEAESR
                     KIGLVFVPEALILAMRRAPCILIDATRAARLEFLVRDYAYLGDDVGALQDKLHRLREH
                     QSRETLERWKSLAAAHALPELFAELIDQHYDPLYHRSQNHNYVHYADALRVESADLSA
                     AGVRALAGRILAEAAE"
     gene            complement(13377..13961)
                     /locus_tag="PKN25_07485"
     CDS             complement(13377..13961)
                     /locus_tag="PKN25_07485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV90777.1"
                     /translation="MSSDPFPEPRRHPSLHGAGGAPSDWVRRFTALLADGARVLDFAA
                     GGGRHARWLAARGCSVTAVDRDAAALATLDGVAGIATRVLELEGADWPLGGERFDAVV
                     VANYLYRPRLAELFELVRPGGVLIYETFMLGNERFGKPSNPDFLLRPRELLDCLPEGW
                     SVVAFEQGEIERPAMIQRLCATRGASPGRLPQSV"
     gene            14231..16705
                     /locus_tag="PKN25_07490"
     CDS             14231..16705
                     /locus_tag="PKN25_07490"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNV90778.1"
                     /translation="MDLAQFTLQYDRPSTDVESLKRAIANKLMYQIGKNPESALPEDW
                     LHAVAYTVRDHLVERWMKTTRASYAQDVKRVYYLSMEFLIGRSLGNAMLALELAGPVR
                     EALRLLDIDPDSLPELEPDAALGNGGLGRLAACFLDSMATLGVPGFGYGIRYDYGMFR
                     QQIVDGQQVEVPDYWLTHGNPWEFPRPEIRMRIRFGGHLQEDGGRVHWVGTDDVLAMA
                     YDSIIPGYGTEVTNTLRLWSARATEEIDLSAFNRGNYFGAVESKNHSENVSRVLYPDD
                     STLEGRELRLRQEYFFVSASLQDILRRYLVGHTDFDALPDKVSIHLNDTHPVLAVPEL
                     MRLLVDEHGLEWARAWALCRRVFSYTNHTLMHEALETWPLEMLGRVLPRHLRMIFDIN
                     AHFLAELNAELGPDVERMRRMSLIDEQGERRVRMAYLAIVASHSVNGVSALHSALMRE
                     SIFADFARAWPQRFNNKTNGVTPRRWLAHANPGLAALIDSRIGPAWRRDLEQLQGLRA
                     HADDADFLHTLRAVKQANKLRLAQWIGQHCGLHVDPAALFDVHVKRIHEYKRQLLNVL
                     HLVSRYQRILAEPNGEHVPRVAIFSGKAASAYRMAKLVIRLINDVAAVINADARVGER
                     LKVVFVPNFSVSLAELIIPAADLSEQISTAGTEASGTGNMKLALNGALTIGTLDGANV
                     EIREQVGEDNIFIFGHTAAEVAQIRNGGYQPRQYYERDARLAAALDAIRAGLFSPDEP
                     GRYQAIFDTLVNWGDHYLLLADYASYLDAQDQADARYRDPLQWSRCSLLNIAGMGAFS
                     SDRTIAEYARTIWHTEPLRIGSPGGL"
     gene            16860..18743
                     /locus_tag="PKN25_07495"
     CDS             16860..18743
                     /locus_tag="PKN25_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNV90779.1"
                     /translation="MTAELETILERHRRDPLQLLQILIELQARDGWLPPATLSALAGA
                     LGIPRARVESTASFYSFLHTRPAGEYRILFSDNITDRMLGNQALMQTLCDKLWLQPGK
                     VSEDGLASVSTTSCTGMCDQGPALLANGRTITRLTLERIDEMAHLIRRRVPVGDWPPE
                     WFHVEDKLRRRDVLLDHGLAPGAALAAALERGPTGLLWEIERSGLRGRGGAGFGSDIK
                     WRSCRDAWGDAHYVICNADEGEPGTFKDRVLLSSYFDLVVDGMCIAGMAIGANKGFIY
                     LRGEYRYLLDRLETRLAQRREQNLLGRNILGRGFSFDIEIHLGAGAYICGEESALIES
                     LEGKPGKPRIRPPFPVTNGYRGQPTTVNNVETLALAALIAVRGGDWFHAIGTPASTGT
                     KLLSVSGDVERPGIYEFPFGVTVAEVLEAAGARDTQAVTSAGAAGHCLAADEFGRRIA
                     FEDVATGGSIMVFDRSRDMFEVAHNFAHFFAHESCGFCTPCRVGTAVNARLLDKLAAD
                     HGSPYDLDEIAKMHRLMQGASHCGLGNTATIAIDDMLAKFRPAFERRLHSPDYEPAFD
                     LDAALSQARRMTGRDDPGAHLGDNHEALVEERPHAQGATTAGAAQSVPSSAEAASAKD
                     LQP"
     gene            18740..19471
                     /locus_tag="PKN25_07500"
     CDS             18740..19471
                     /locus_tag="PKN25_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNV90780.1"
                     /translation="MNDSFLLDGEDIPFTPGQTVMQAAAAAGKYIPHLCWHPDFAAHG
                     SCKLCTVKIGGRFATSCTVRAAAGQEVENRTAELDAKRRTLLQLLFVEGNHFCPSCEK
                     SGDCALQATAYEMGMLTPHFDHFYPDRPVDASHPDVLLEFNRCILCELCVRASRDVDG
                     KNVFALAGRGTQSHLVVNSDTGRLADTDFAVTDRAAAVCPVGVILSKRVGFAVPIGQR
                     TYDRRPISASTSPEGKAGAGIEEAR"
     gene            19471..>19772
                     /locus_tag="PKN25_07505"
     CDS             19471..>19772
                     /locus_tag="PKN25_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNV90781.1"
                     /translation="MDIDIAKIDAGGAPRKLKVATVSLAGCFGCHMSFLDIDERLLPL
                     LDLIEFDRSPLTDIKHCGPCDIGLIEGGVCNAENVHVLREFRANCKVLIALGACAV"
BASE COUNT         3225 a         6904 c         6431 g         3012 t
ORIGIN      
        1 gcacgagcat ctcgaagagg tggcggtcgt cgtgattggg cacgccccac tcggtatcgt
       61 ggtaggcgac gtagagcgga tcggtgccgc accaggcgca gcgctcggac atggggaact
      121 ccggtcgggg ggtgcggaaa acgctatgcg atcgcgaagc gcgaagggct cagtctaccg
      181 gttcgaggca gatgtggcgg cggcgatggg gatcctgtcc ccatcccggt taccgggggt
      241 agcctccacg aaccatccga cgttgtgctg catcctgccc gcgcaccagc gcctcctcca
      301 cgctcaggcg tccggcgagc gcttcggcga tcaggttacc gacttcgtcg ccaatgagga
      361 tgaactcggg gatcgccgcg aactgaatgc ccgggtaagg gcttggtggc agggtggcgt
      421 cttccggatc tgcatcgagc atcgcttcga gctcatgcac agcccagggc gccttgcgct
      481 gaaactccgg ccgagcatag gtcgatctgc gggtacccgt cggcacggcc cgccaaccct
      541 gccgggccgc aacgagctcg acgtagtcgc gtgaagccac ccagtggatg aacttccgag
      601 cgtcagcctc ccgaacgggg tcgatgctgc tcgggatcgc cagcgcccaa gcccacagcc
      661 attgcgcacc cttggccgtc acctcgtacg gagcggctgc gaagccgacc tcaccggcga
      721 ctgcactcga ctctgggttc gtgaggactt ccgcagcgac cgtcggcccg acccattgag
      781 cgcatctgcc cgacgcaaac agcgagaggt tctccccgta gcccagtgaa gccgcatcgg
      841 gcggtccata tcgattcagg agttccgcat acagcccgac cgcctttttc cacggcagac
      901 tgtccaactg gggtcgccag cccgcatcga accattggcc gccaaacgaa ttgaccatgg
      961 tgctcaccag cgccatgttc tcgatccagc ccgggcggcc acgcaaacag attccatgca
     1021 cgccattcgc agggtcgtgg agcttcgcgg caaacgcggc aacctcggtc caggtcggcc
     1081 tgtcgggcat ggtgaggccc gcctttttca gcagatcctt gcggtacatg agcatggagc
     1141 tctcgccgta gatgggcgcg gcatacagct cttgctggta ggaaagcgct ttgcggacgg
     1201 tcggcagcag atcgtccacg tcgaaatcgt ccggcgaccg caagggtttg atccatccgc
     1261 tggcagccca gatcggtact ttgaagattc cgatggtaat cacgtcgaat aggccgtctc
     1321 gcgtctcgac gtcggcactt gccacccgcc tcagctcgga ctcctcgaga acgacccacc
     1381 ggatgcgaat ctccggattg gccttctcga actcgctgct gagggtttgc aggcgacgca
     1441 tgtgggcgct gtcgacggtc gcgatcacca aggcgccggc acgcacaggc agggccacga
     1501 acagcgtcag agccgcgaga acgaaacaca aggcgaggca gctagaccgg gatttcatca
     1561 cgacacctgc gagctcgcgc gctcgcgcac tgccgcctcg aaggcgtcca cgggcaaggg
     1621 cttgccgaac aggtagccct ggaaagcatg acagccctgg gcgacgagat attcatgctg
     1681 cgcacgggtc tccaccccct cggcaatcac gtcgagttcc aagccctgcc ccatggcaat
     1741 gatcgtctgg gccaccatag cgtcgcgatg gctggccggc agctcgtcta caaaggactt
     1801 gtcgatcttc agctgcgaga gcggcagctt cgtcaggtag ttgagcgagg agttgccggt
     1861 gccgaaatcg tccagagaga tcctcacgcc gcgagcccgt agctgtcgaa gcttgtccac
     1921 cgacgcatcg aaatcatcga ttgcaaggct ctcggtcacc tccagcttca acgttctggc
     1981 ggggacgccc gcgcgttcga gttcatcgaa tacatcctga acgaaactcg ccgccttgaa
     2041 ttgcggcgga ctcacgttga cagcaagggt gagccgactg aaaagatcgt catacgacca
     2101 ctgtgcgagc tgctggcatg ccgtggcaag cacccagcgg ccgatcggct cgatcagccc
     2161 ggtttcctct gccagcggaa tgaacacaaa cggaggcacc agaccccgca ccggatggat
     2221 ccagcggatg agtgcctccg caccgatcgg gagaccctcg gcatcgacct gcacctgata
     2281 gtgaagtacg agttgatggt tctcgatggc ctcgcgcagt tcctcttcca gcaggttgcg
     2341 gcggtcgagt tccgactgca tcgcgtcctc gaagaagcgg accacgttgc ggcccatatt
     2401 cttgcccttg tacatcgcca gttcggcttg gcgcagcaag gtttccggcg aagcttcccg
     2461 cccccggaaa agcgtcacgc caatcgttgc ggtgctcgag atccgacgtc cctcgatcgt
     2521 catcggcacc gcgatcgcct gcctgaccgc ctcagcaagg tcctgggcgc gtcccgcggc
     2581 cgcctggcga tcgtgcccaa ggttctcgag catcagcacg aagctgtcgc ccgaaaagcg
     2641 gcccagcgtc tcgccgttgt ccgcaacgcc ctgcatgcgc cgcaccatct cggtcagcag
     2701 ccggtctccc accccgtggc cccacgagtc gttcaccttc tggaacagat cgaggtccag
     2761 catcatcaca gcaccgagtt caccgcgttc ggcgctgttg gccagggcat gggtgatacg
     2821 gtcttcgagc aggctgcggt tgggcaggtt ggtgagctga tcgtaaagct tgagacgctc
     2881 tgccagctcc cgcgcctgcc tctcggccga aatatcctgg tagctgcaca catggtgaag
     2941 cggccgcccc tgcgtgtcgc acactgcggt gatcgacagc cgggtgatga acaggccgcc
     3001 gtcacgatgc cgattcacga tctcgccggc ccagtagccc ttgcgcgcta cggcacccca
     3061 catggccgag tagaagtccg gatcgtgcac atccgacttg aggaagcgcg gcgtgcagcc
     3121 gacaacctcc tccgccggat agccgctcat gcgtgtgaat gccgagttga tgcgttcgat
     3181 caccccgcgg ccgttggtga tcatcatgcc atcctggctc tcgaacgccg ccgctgcgat
     3241 gcgcaattca ctttccgcac ggcggcgcgc ggtcaggtcg tgcgcgacga cgagcacgcc
     3301 gtcgatcgcg ccatcggccg ccaagcgtac tggcaccttc acgatctcca gttcgtaggc
     3361 ggttccgtca gcggcctcga accgcctttc gtaccgatga ctcgctcctt ggccaaggac
     3421 ctccgcgtcg ttcttccgga cggtggcgac gaagtccttg tcctcgaaca actggtcgtc
     3481 gctgcgaccg atgatctgtg ctcgcgccag gccgaagcca cgtgcgagca cggcattgca
     3541 gtcgacgtag actccattgc ggtccttgag cgccatgcca tccgggctgc tatcgaagat
     3601 ggcgcgcagt tgcgccttct cgaccacgag ctcgcgtgct gcttgctcca atgcctcgtt
     3661 cttttggtgg atctcggcgg tcctcgcata caccagtccc cgaaggaagc ggatccagag
     3721 catcgccacg gcgagcacgg ccagagcgat ccccgcaacc atgaggatga tgcgaagata
     3781 gctccggaac tcgagcggct tcgcaagcca cttcttgctc agcgcttcga gttcggcagg
     3841 cgtgatccgc gccatgcctt cctgaacgcg ggcaaaggtg cccgcgtcgc ccttcgcgac
     3901 cgcccaatgg aactggcctt cgtacagggt gaatgcgcgg gtgaacttca cctgatcgcg
     3961 gtacaggtag aggtagtagt tggccggctt ctcatccatg cagaacatct tgatgtcgcc
     4021 gtcccgcgcc gcagccagga ccgcctcgta gctcgcataa ggaaccagat tggtgacacc
     4081 gagcgtggtc agcctgtcga tgcaggcatc acccctctgc accgcgacgg cgaagccctg
     4141 catggatccg gcgtcgacaa tcccgttgat cgaagggtcg acaaagatcg acaccgggag
     4201 cgtggcgtag ggctgggaga agtcgtagag ctgctcgcgg accggtgtgc ggaagatcat
     4261 atcgatcacg tcggcacggc cgtcgaggat ggcccgctgc gcgtccgccc attgcatggc
     4321 ctgcaaagcg accgaggcgc cggtcttctc ctcccacagc ttccacagat cgacgacata
     4381 cccctgcggg cttccgtccg gaccaggaaa cacgtacggc gggtagttgt tgtcggtgac
     4441 cacccgcagc ttgccgccgt cagcctggac gccgaaagaa agcgtcgcga gcgccacggc
     4501 gagcaacctg gaaagactcc ttccaagaag gagcatccgc accgaaatca tcacttcctc
     4561 caggacgagc acccgatgag cctggaagct ccatcctcga cacgccaccc tcagcatgaa
     4621 ctcataatag atttcttggc atatatcgag aaatactatt tctgacaaat tgcaacaaaa
     4681 ttcacaggga atcgaggcgt cgccgccgac ggatgcgggc caaggccggg acggtaggag
     4741 acgtgaaggt ttggtgtcgc ctgctgcgaa cggaggctgc atccagcatc tgcccgttca
     4801 tcgcctgcca gcttggcatc caatgcggcc gtcagcgccc gtctcgtcct ttccctgacg
     4861 gcggctccac ctggctccac acgctttcca cgcgcgggac cgagcgcata cgccgatacg
     4921 gaagaccctt cccctcaccg gttcacccac cccccgcaca ccggaaacac ctgccccacg
     4981 aagcagttcg ccgcctcgct gcacagatag accgcaaagc tggcgtcctc gtccgccgtg
     5041 accaggcgcc caagcggcac ctgccacttc aggcggtcct tgaacgccgg cgtggcctgg
     5101 acctcgggcg ggaagtaggt ggggttctcg acgaaattct gcgcaatggc gttgacctgg
     5161 acgccatgcg ccgcggcctc gacgcccacc gcctggatgt aggccagttg cgcgccgcgc
     5221 gcggcggcgt agctcgagcg cagggccatc ccgcgcaggg cggcggcgct gcccatcagg
     5281 aggatcttgc ccgccttgcg ttcgatcatc tgcggcagga cggcggcgac catctcccgc
     5341 atgggatgga ccagggcggc gaaggtggtc tcccattcgc cgtccgacac ctggtggacg
     5401 ggcgtgctcg gcgcgggaat ggccaggttg aggaccagca cgtcgatccg gccggccgcc
     5461 tcgatgaccg cgcgccctgc cccccgctcc agcagcgcgc ttcgatcggg gacgacttcg
     5521 gctcccgcag cgcggaaggc gtcgcacagt gccgggccca tgaacgcgtc ggcctgcgtg
     5581 accagcaccc tgcggccgct caatgaattc gcatgcatgt cggttctcct cggctgctgg
     5641 gcggggcagc ctccccacgg ggcgcggcac gatcgtcggc gaagcggcgg cccggcagcc
     5701 ggcagttttc tcctgcgcga gcgcgattcc ggcccctacg ttccatccta gatgaagacg
     5761 tctaaactgc atcgagaggg atcgatgtgg cacaggcgca cgccgccgcc acgatttcga
     5821 cacaccggaa gaggttccgc gcgatgccca ggatcgagcc gtttgaagcg catgcagcgc
     5881 gttacgacgc gtggtttgaa cgccaccagg cagcctatgt ctccgaactg ctcgcgctgc
     5941 gatccttcgt cccgctgagc gggcgcggac tggagatcgg cgtcggcagc gggcgcttcg
     6001 ccgcgccgct gggggttcag gtggggatcg acccgtcgcg gacgatgctt gagcgcgcgc
     6061 gtgcccgcgg ggtcgaagtc tgcgagggca ccgccgagca tctgcctttc gccgagcaca
     6121 gtttcgatca cgcgctcgtc gtcaccacct tgtgcttcgt cgattcgccg gcggcgatgc
     6181 tgacgcaggc gcggcgggtc ttgaagccgg gcggcagcct ggtgatcgcg ttcatcgacc
     6241 gggacagccc gctgggtcgc gactacgagg cgcaccgcga gaccagcgat ttctaccgcg
     6301 atgcgcactt ccattcggcg ggcgaagtgg agcgcctgct gctggatgca ggcttttccc
     6361 tgcggtcgtg ggcgcagacg ctggcccggc cgctcgcgga gaacgnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnggctt ttccctgcgg tcgtgggcgc agacgctggc
     6541 ccggccgctc gcggagaacg ggcacatcga gccggcgcgc cccggtcggg gcgaaggcgc
     6601 tttcgtggtc gtcgatgcgg tgcggccctc gtgatctgtc gccggccacc gatggggaca
     6661 gcagcgcgcg ggagcgcgac atcggaatct tcacggcgga aaggcatcca caacatgcgc
     6721 cacacccact tccaagggat cgtccctgcg tggaggagat cgacatgaag caagtgcaag
     6781 cgatacgacc ggaccgtgaa gtcctgacgc gtcagcggct gccgtatttc atcgggatct
     6841 cggggcagac ggtcggggca agcgggctga gcatgcatct ggtgacgatc ccgcccgggg
     6901 cccggtcgga gccgcatgtc cacgtcggct acgaaaccgg aatctatgtg ctcgaaggca
     6961 gggtcctgac gcgctggggc gcgaagctgg aaaacgaggt gatcagcgcg gcgggggagt
     7021 tcctgttcgt tccggcgggg gttccccacg aggcgatcaa tctcagcgcc actgaagcgg
     7081 cgcgcgcggt ggtggcccgc aaccatccgg cagagcagga ccaggttcag ccctacgttg
     7141 ccgggtaggg gctcggcgcg gcggatccat caagcggcgc ggcttcgatg cgtgccggcc
     7201 gatcccttgc gccgcgcaaa tctccgcgga catgccccgg cacgggcgtt acaattgcag
     7261 gctttccagg cagcaatcgg agtgcccgca atgcccaccc agaccgaaaa cctcaacgtc
     7321 ctcgccttcg accacatgcc ctcgccggac gaggtgaagg cgcgcgtgcc gctgaccgag
     7381 cgcgccgcgg ccgcggtggt ggccgggcgc aaggcggtga tggacatcct cgatcgcaag
     7441 gatccgcgcg tcttcgtggt ggtcgggccg tgctcgatcc atgatcccgt ggcagggctg
     7501 gattatgcgc ggcggctgaa ggcgctcgcc gacgaggtct ccgacgtgct gctgctggtg
     7561 atgcgggtgt atttcgagaa gccgcgcacc tcgaccgggt ggaagggcta catcaacgat
     7621 ccgttcatgg acgactcctt ccgcatcgac gtgggcatgg agcgtgcgcg caaattcctc
     7681 ctcgacgtgt gcgagctcgg cctgcccacg gccaccgagg cgctcgaccc gatcgcaccg
     7741 cagtattacg gcgacctcat cgcctggacc gcgatcggcg cgcgcacctc cgagtcgcag
     7801 acccatcgcg agatggcctc gggcctgtcg acgccggtcg gcttcaagaa cgccaccgat
     7861 ggcgacctcg aggtggcgat caacgcgatc atttcggccg gcagtccgca cagcttcctc
     7921 ggcatcaaca gccagggcca gtcggcggtt acccgcacgc gcggcaaccg ttacggccac
     7981 gtggtgctgc gcggcggcgg cggccggccc aactacgaca cggtgtcggt gtcgctggcc
     8041 gagcaggcgc tcgcgaaggc caagctggcg aagaacatcg tggtcgattg ctcgcacgcc
     8101 aactcgtgga agaagcccga ataccagccc ctggtgatga aggacgtgat gcatcagatc
     8161 cgcgagggca accagtcgat cgtcggcctg atgatcgaga gcaatatcga agccggcaac
     8221 cagccgattc cggccgacct gtcgcagctc aagtacggct gttcggtcac cgatgcctgt
     8281 gtcgattggg cgacgaccga ggacatgatc cgcaagtccg ccgccgtgct gcgcgacgtg
     8341 ctgccgaagc gggagcggcg cgcatgaacc tcgtcgtgct ggccgaggac gtcgacagcg
     8401 tcgcgctcaa gaacgtcgcc aaggtgtcgg aggcgagcgc gatcgagcag atcaccacgc
     8461 gctccttccg cctcgtcggc gcgaagccgg tcgaaggtct ggtggccgcg tgcgcggccg
     8521 gggggctcga ccacgtgtgg ctgcccgagg gccgccagct gcgcgatttc ggcctcttcg
     8581 tgaccgacat ggactcgacc ctgatcaaca tcgagtgcat cgacgagatc gccgacatgc
     8641 agggcttcaa acgcgaggtg gcggcgatca cggaggccgc gatgcagggc gagatcgact
     8701 tccgcgagtc cctgacgcgg cgcgtggccc tgctcgccgg cctgcccgag agcgcgctcg
     8761 ccgaggtctt cgatcaccgc ctgcagctca accccggggc ggagcgcctg ctgcaggggt
     8821 tgcagcgcgc gggtatccgc acgatgctgg tgtcgggcgg cttcacgtat ttcaccgagc
     8881 ggctgaagtc gcggctgggc ttcgactatg cgtgggcgaa ccagctcgag gtgtatgacg
     8941 gaaagctgac cggaaaggtg ctcggggaca tcgtggacgg cgaggccaag gccgcgcacc
     9001 tggagcaggt gcgcgacgag ttgggcctca ggcccgagca ggtgatcgcc gccggcgacg
     9061 gcgccaacga catccccatg ctgcgcgcgg ccggcttcgg tgtcgccttc cacgccaagc
     9121 cggtgctgcg cgacgtcgcc cactgctgcc tcgaccacac cggcctggac ggcatcctcg
     9181 acctgttcgg ccctggcggc gaaagctgaa gccgcacgcg gcccccgttt tcgtnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtagga gcgacgcaag tcgcgattcc
     9361 ggcccccgcg cctccaccgc cgcgtcatcg ccaccccacc gcccgatcgc gactggcgtc
     9421 gctcctacgg ggcctcgctg aggcggccgg cgtccatgcg tagcacgcgg ccgcagcggc
     9481 gggcgaggga ctcgtcgtgg gtcaccagga tcagcgtggt gccctgctcg cggttgagcg
     9541 cgaacatcag ctcgatgatc gccctccccg tctcggcgtc gaggttgccg gtgggctcgt
     9601 cggcgagcag cacccgggga tcgggcgcga aggcgcgcgc cagcgccacg cgttgttgct
     9661 cgccgcccga caggtgcttg ggataatgcg tgagccggct gcccaggccg acgcgctgca
     9721 gccattgggt ggcgatcgcg cgcgcgtcgt ccacgccggc gagctccagc ggcagcatca
     9781 cgttctccag cgcggtgagc gccggcagca gctggaagga ctggaacacg aagccgatcg
     9841 cctcgccgcg cagcgccgcg cgcgcgtcct cgtcgagcgc gaagaggtcg cggccctgca
     9901 cccgcaccgt gccgtgcgcg ggaacgtcca acccggcgag caatcccagc agggtcgatt
     9961 tgccggagcc ggaggcgccc acaatcgcca ccgactcgcc cgcacccacg gcaaagcgga
    10021 tgtcctgcaa aatccgcagc tcgccctggc cgtcggccag cgcgacctgc ttgcacaggt
    10081 catcgacctc gatgaccgga ttggagagct cgatgccgtt gcgtttcatc attcccttcg
    10141 cgttcatgtt ccttttcggc gccgtcgccc gcgccgacac catcctggta tggggcgaca
    10201 gcctgtcggc cggctacggt ctggaaaggg gccaggaatg gcccaccctg ttgcagacgc
    10261 gtctgcaggc cgaagggttc cgccatgtcg tggtcaacgc cagtgtatcc ggcgagacct
    10321 cgtcgggtgg cctcacccgc ctgcccgccg ccctggccga acatgcgccc acgctggtga
    10381 tcctggagct cggcgccaac gacggcctgc gcggactcaa gccgcagctg ctcgcggaca
    10441 acctggccgc gatgatcgac gctgcgcacg cggcgggcgc gcgcgtcctg ctgatcggca
    10501 tgcgcatgcc gccaaactat ggtccggcct acacgcaacg cttcgcagag gtcttcgcca
    10561 acgtggccgc ggagaagaag gtgccgctcg tgcccttcct gatggacggc ttcgccgacc
    10621 gccccgagct cttcctgccc gacggcatcc acccggccgc cgaggcgcag ccgcgcatcg
    10681 tcgacacggt atgggccggc cttcgcccgc tgcttgccgc gaaggagacg aaggcggcgc
    10741 gcaagtgagg ctcgaagcgc cggggggaat tcagccgcag gagcgctgcg aggcgcgatc
    10801 gtggcgcacg ggatccgacc gacgcccgtc ccggcacgat cgcccgatcg cgactcgcgt
    10861 cgctcctacg aagaaccgaa acaccgaggg ctggctgtag gagcgacgcc agtcgcgatc
    10921 gcactgcccg gtcgcgcgtc gcggccgaag gcaggggagc acggcgcccg atgccggacg
    10981 accgcccctc cccggaaacg cccgtggcgc ttacgccacc accacccgcg ctgcgcccgc
    11041 cttcatctcg ccgacgatcg tccccgcctc gccctgctcg gcgcgcaccg cggcctgcac
    11101 ggcctcgacg gcttccggcg cgcaggcgat cagcaggccg ccggaggtct gcgggtcggt
    11161 gacgagcttg cgctgccact ccggggcgtc caccggcagc tccacgctgg caccgtagct
    11221 cgcccagttg cgcgtcgacg cgccggtggc gacgccgtcc cggaccaggg cgcgcgcctc
    11281 ctcgatcacc ggcagcgcat cgaagcgcac cgcgccggtc agcgacgcgc cgcggcacat
    11341 ctccagcagg tgccccgcga ggccgaagcc ggtgacgtcg gtcaccgcgt gcacgccgtc
    11401 gaggtcggcg agcctggcgc cgacacggtt gagcgtcgtc gtccagcgga tcatctgcgc
    11461 gtaaccgtct gccgacagcc ggcccttctt caagcccgcc gagaggatgc cgatgccgag
    11521 cggcttggtg aggatgagca cgtcgcccgc cttggcgccg gcgttggtgc gcacgcgcgc
    11581 ggggtcgacc acgccgaggc cgaccagacc gtagatcggc tcgagcacgt cgatcgagtg
    11641 cccgccggcg accgggatgc ccgcgtcgcg gcagacctcg gcgccgccct tgaggatctc
    11701 gccgatcacc gcgggcggca gcttgtcgag cggcatgccg acgatcgcca gcgccaggat
    11761 gggctgggcg cccatcgcat agacgtcgga gagcgcgttg gtggcggcga tgcggccgaa
    11821 gtcgcgcggg ttgtccacga tcggggtgaa gaagtcggtg gtggcgacga tggcctggcg
    11881 ctcgttgagc ttgtagacgg cggcgtcgtc ggcagtgtcg gtccccacca gcagctcggg
    11941 tggcacgagg ccggcgggca tggtggcgag cagctcggcg agcacggcgg gggcgatttt
    12001 gcagccgcag ccgccgccgt gggagaattc ggtcagtctg atttcgttca tgtttccggg
    12061 agagaaagcg acgcgcggcg caagtgggcg ccgcgcgtcg tgtcaacatc atgcaaaaag
    12121 gcgtcgcgac cgtagcacag ctgtccggct ttgacgagat catcgacgcg cgcacgcccg
    12181 ccgagttcgc cgaagaccac attcccggcg cgatcaacct gccggtgctc gacaacgacg
    12241 agcgcatcgt cgtcggcacg atgtacaagc agcagtcggc cttcgacgcc cgccgcctcg
    12301 gcggcgcgct ggtggccgcc aacatctcgc gccacatgct cgccacgctg caggacaagc
    12361 ccaagaactg gcgccccctc atctactgct ggcgcggcgg ccagcgcagc ggctccttca
    12421 ccacctggct gcgcatggtg ggctgggacg cctgccagct cgagggcggc tacaaggcct
    12481 tccgccacct ggtgatcgcc gagatcgagc gcatcgtgcc cacgctggcg ttgcgcatcg
    12541 tgtgcggccc gaccggcagc gcgaagacgc gcgtgctcga ggcgctcgcg gcacaagggg
    12601 cgcaggtgct cgacctggag gacctcgccg cgcacaaggg ctcggtgctc ggcgccctgc
    12661 ccgaccgtcc gcagccggcg cagaagcgct tcgagaccct gctgtgggat cgcctgcgcc
    12721 ggctcgatcc ggcgcgcccg gtgtatgtcg aggccgagag ccgcaagatc ggcctggtct
    12781 tcgtgcccga ggcgctgatc ctggcgatgc ggcgcgcgcc ctgcatcctg atcgacgcca
    12841 cgcgcgcggc ccgcctcgag tttctggtgc gcgactacgc ctacctcggc gacgacgtgg
    12901 gcgcgctgca ggacaagctg caccgcctgc gcgagcacca gagccgcgag acgctcgagc
    12961 gctggaaatc gctcgccgcc gcacacgcgc tccccgagct gttcgccgag ctgatcgacc
    13021 agcactacga cccgctttac catcgttcgc agaatcacaa ctacgtgcac tacgccgacg
    13081 cgctgcgggt ggagagcgcc gacctgagcg cggccggggt gcgcgcgctg gccggtcgca
    13141 ttctcgccga agcggcggag tgaagcgtcc ggggcgagcc cgagcagccc cgccacccca
    13201 atcgggacgc gcgtttcctc gaagaaccgg cgcgttccct gtggcgccga cgtccgcatc
    13261 ggccggtggg agcgacgcga gtcgcgatcg ggtcggcccg ttgcggcgac gcaggtcgcg
    13321 atctctccgg ccgaggggcg gacaccgtcg catcttcgcc caggtggccg ccaagcctag
    13381 acggattgtg gcaggcggcc cggcgatgcg ccgcgggtcg cgcacaggcg ctggatcatc
    13441 gccggacgct cgatctcgcc ctgctcgaag gccaccacgc tccagccctc cggaaggcag
    13501 tcgagcagtt cgcgcggacg gagcaggaaa tcggggttcg agggcttgcc gaagcgctcg
    13561 ttgccgagca tgaaggtctc gtagatcagc acgccgcccg ggcgcaccag ctcgaaaagc
    13621 tccgccaggc gcggccgata caggtaattg gcgaccacca cggcgtcgaa gcgctcgccg
    13681 ccgagcggcc agtccgcacc ctcgagctcg agtactcgcg tcgcgatccc cgccacccca
    13741 tccagcgtcg ccagcgccgc ggcgtcccga tcgaccgcgg tgacgctgca gccgcgcgcc
    13801 gcaagccagc gcgcatgccg gccgcccccc gcggcgaagt cgagcacgcg cgcgccgtcc
    13861 gcgagcagcg cggtgaagcg ccgtacccaa tcggagggag cgccgccggc accgtgcagg
    13921 ctggggtgac ggcggggttc ggggaaggga tcggaagaca tgggggctcc ttggatgcaa
    13981 gcctcgtcgc gggggcgcgg caagaccggg cgtggccacc gcattctagc gacgcacggc
    14041 atgccgtgcg tcgcaggctt gccctcccac ctcgcgctgc ccgcgcgccc catgcccggg
    14101 accgacgcac aggcacgggc gggcgatcga gcgccattgc aggcgaaagg cctaaactag
    14161 cggatttcgc cacgtcacca cgccggccgc ggccgcgcca cccgattacc ggaccttcac
    14221 cgacgtcacc atggatcttg cccagttcac cctccagtac gaccgcccca gcaccgacgt
    14281 cgagtcgctc aagcgcgcga tcgccaacaa gctgatgtac cagatcggca agaaccccga
    14341 gtcggcgctg ccggaagact ggctgcacgc agtcgcctac accgtgcgcg accacctggt
    14401 cgagcgctgg atgaagacca cacgggcgag ctacgcgcag gacgtcaagc gcgtctatta
    14461 cctgtcgatg gagttcctca tcggccgcag cctgggcaac gccatgctcg ccctcgaact
    14521 cgccggcccg gtgcgcgagg cgctgcgctt gctcgacatc gaccccgaca gcctgcccga
    14581 gctcgagccc gacgccgcgc tcggcaacgg cggcctcggt cgcctcgccg cctgctttct
    14641 cgactccatg gccacgctcg gcgtgcccgg cttcggctac ggcatccgct acgactacgg
    14701 catgttccgc cagcagatcg tcgacgggca acaggtcgag gtgcccgact actggctcac
    14761 gcacggcaac ccgtgggagt tcccccgccc cgagatccgc atgcgcatcc gcttcggcgg
    14821 ccacctgcag gaggatggcg ggcgcgtgca ttgggtgggc accgacgacg tgctggcgat
    14881 ggcctacgac tcgatcatcc ccggctatgg caccgaggtg accaacaccc tgcgcctgtg
    14941 gtcggcacgc gccaccgagg agatcgacct gtcggccttc aaccgcggca actattttgg
    15001 cgcggttgag agcaagaacc actccgagaa cgtctcacgc gtgctctacc cggacgactc
    15061 caccctcgag ggtcgcgagc tgcgcctgcg ccaggagtat ttcttcgtct ccgccagcct
    15121 gcaggacatc ctgcgccgct accttgtcgg ccacaccgac ttcgacgccc tgcccgacaa
    15181 ggtcagcatc cacctcaacg acacccaccc ggtgctcgcg gtgcccgagc tgatgcgcct
    15241 gctggtcgac gagcacgggc tggaatgggc gcgcgcctgg gcgttgtgcc ggcgggtgtt
    15301 ctcctacacc aaccacaccc tgatgcacga ggcgctcgag acctggccgc tggagatgct
    15361 cggccgcgtg ctgccgcgcc acctgcgcat gatcttcgac atcaacgcgc acttcctcgc
    15421 cgagctcaac gccgagctcg ggcccgacgt cgagcgcatg cgccgcatgt cgctgatcga
    15481 tgaacagggc gagcgccgcg tgcgcatggc ctacctcgcg atcgtcgcct cgcactcggt
    15541 caatggcgtt tcggcgctgc attcggcgct gatgcgcgag tcgatcttcg ccgacttcgc
    15601 gcgcgcctgg ccgcagcgct tcaacaacaa gaccaacggc gtgacgccgc ggcgctggct
    15661 ggcgcacgcc aacccagggc tggcggcgct gatcgacagc cgcatcggtc cagcctggcg
    15721 gcgcgacctc gagcagctcc agggcctgcg cgcgcacgcc gacgacgccg acttcctgca
    15781 cacgctgcgc gcggtcaagc aggccaacaa gctgcgtctg gcgcagtgga tcggccagca
    15841 ctgcgggctc cacgtcgatc cggccgcgct cttcgatgtt cacgtcaagc gcatccacga
    15901 gtacaagcgc cagctgctca acgtgctgca cctggtgagc cgctaccaac gcatcctcgc
    15961 cgagccgaac ggcgagcacg tgccgcgggt ggcgatcttc tccggcaagg cggcctcggc
    16021 ctatcgcatg gccaagctgg tgatccgcct catcaacgac gtggccgcgg tcatcaacgc
    16081 cgatgcgcgc gtcggcgagc gcctcaaggt ggtcttcgtg cccaacttca gcgtcagcct
    16141 ggccgagctc atcattccgg ccgcagacct ctccgagcag atctccaccg ccggcaccga
    16201 ggcctcgggc accggcaaca tgaagctcgc actcaacggc gcgctcacca tcggcacgct
    16261 ggacggcgcc aacgtcgaga tccgcgagca ggtcggcgag gacaacatct tcatcttcgg
    16321 ccacaccgca gccgaggtcg cgcagatccg caacggcggc taccagccgc gccagtacta
    16381 cgagcgcgac gcgcgcctgg ccgcggcgct cgacgcgatc cgggcgggcc tcttcagccc
    16441 ggacgagccg gggcgatacc aggcgatctt cgacaccctg gtgaactggg gcgaccacta
    16501 cctgctgctg gccgactacg ccagctatct cgacgcccag gaccaggccg acgcgcgcta
    16561 ccgcgatccg ctgcaatgga gccgctgcag cctgctcaac atcgccggca tgggggcgtt
    16621 ctcgtccgac cgcaccatcg ccgagtacgc gcgcacgatc tggcacaccg agccgctgcg
    16681 catcggcagc ccgggcgggc tatgaaggca cgcgggcggc ggcatggggg ccgcccgcgg
    16741 cgcgatcggc acgaataagt gcaatgcatc ggccccgcgc acgctttcca tgagcgtcgc
    16801 ggagtgcggc gggcgataat cgcgcggcag gggcacgcaa tcacagggag cttgcggcga
    16861 tgacggcaga actcgaaacc atcctggagc gtcaccggcg cgatccgctg cagttgctgc
    16921 agatcctgat cgagctccag gcccgtgacg gctggctgcc gccggccacg ctctccgcgc
    16981 tggcgggcgc gctcggcatc ccgcgcgccc gcgtcgagag caccgcgagc ttctacagct
    17041 tcctgcacac ccggccggcg ggcgaatacc gcatcctgtt ctccgacaac atcaccgacc
    17101 gcatgctcgg caaccaggcg ctgatgcaga cgctgtgcga caagctctgg ctgcagccgg
    17161 gcaaggtgtc ggaggacggc ctggcgagcg tgtcgaccac ctcgtgcacc ggcatgtgcg
    17221 accaggggcc ggcgctgctg gccaacgggc gcacgatcac ccgcctcacg ctcgagcgca
    17281 tcgacgagat ggcgcacctg atccgccgcc gggtgccggt gggcgactgg ccgccggagt
    17341 ggttccatgt cgaggacaag ctccgccgcc gcgacgtgct gctcgaccat ggcctggcgc
    17401 cgggtgcggc gctcgccgcg gcgctcgagc gcggccccac cgggctgctg tgggagatcg
    17461 agcgctccgg actgcgcggc cgcggcggcg cgggtttcgg cagcgacatc aagtggcgct
    17521 cgtgccgcga cgcctggggc gacgcgcact acgtgatctg caacgccgac gagggcgagc
    17581 ccggcacctt caaggaccgc gtgctgctgt cgagctactt cgacctggtg gtcgacggca
    17641 tgtgcatcgc cggcatggcg atcggcgcca acaagggctt catctacctg cgcggcgaat
    17701 accgctacct gctcgaccgc ctggaaacac gcctcgccca gcgccgcgaa cagaacctgc
    17761 tcgggcgcaa catcctgggc cgcggcttct ccttcgacat cgagatccac ctcggcgccg
    17821 gcgcctacat ctgcggcgag gaatccgcgc tgatcgagtc gctcgaaggc aagccgggca
    17881 agccgcgcat ccgcccgccc ttcccggtca ccaacggcta tcgcgggcag ccgaccacgg
    17941 tgaacaacgt cgagacgctc gccctcgccg cgctgatcgc ggtgcgcggc ggcgactggt
    18001 tccacgccat cggcaccccg gcctcgaccg gcaccaagct gctgtcggtg tcgggcgacg
    18061 tcgagcgccc gggcatctac gagtttccct tcggtgtcac cgtggccgag gtgctcgagg
    18121 ccgccggcgc gcgcgacacg caggcggtga cgagcgccgg cgcggccggg cattgcctgg
    18181 ctgccgacga gttcggtcgt cgcatcgcct tcgaggacgt cgccaccggc ggctcgatca
    18241 tggtgttcga ccgttcccgc gacatgttcg aggtggcgca caacttcgcc cacttcttcg
    18301 cccacgagag ctgcggcttc tgcacgccct gccgcgtcgg caccgcggtc aacgcccgcc
    18361 tgctggacaa gctcgccgcc gaccacggct cgccctacga cctcgacgag atcgcgaaga
    18421 tgcaccgcct gatgcagggc gccagccact gcggcctggg caacacggcg acgatcgcga
    18481 tcgacgacat gctggccaag ttccgccccg ccttcgagcg ccgcctgcac tcgcccgact
    18541 acgagcccgc cttcgacctc gacgcggcgc tgtcgcaggc gcgccggatg accggccgcg
    18601 acgaccccgg cgcccacctc ggcgacaacc acgaggccct ggtcgaggag cgcccccatg
    18661 cgcagggcgc caccacagcg ggcgccgcgc agtccgtccc ctcctccgcc gaggccgcct
    18721 cggcaaagga tctgcagcca tgaacgattc cttcctgctc gacggcgagg acatcccctt
    18781 cacccccggc cagaccgtga tgcaggccgc cgccgccgcg ggcaagtaca tcccgcacct
    18841 gtgctggcac cccgactttg ccgcgcacgg ctcgtgcaag ctgtgcacgg tgaagatcgg
    18901 cgggcgcttc gccacgtcct gcacggtgcg cgccgccgcc ggccaggagg tggagaaccg
    18961 cacggcagag ctcgacgcca agcgccgcac gctgctgcag ctgctcttcg tcgaaggcaa
    19021 ccacttctgc ccttcgtgcg agaagagcgg cgactgcgca ctgcaagcca ccgcctacga
    19081 gatgggcatg ctgacgccgc atttcgacca cttctacccc gatcgcccgg tggacgcctc
    19141 gcaccccgac gtgctgctcg agttcaaccg ctgcatcctg tgcgagctgt gcgtgcgcgc
    19201 cagccgcgat gtcgacggca agaacgtgtt cgcgctcgcc ggccgcggca cgcagtcgca
    19261 cctggtggtc aacagcgaca cggggcggct cgccgacacc gacttcgccg tcaccgaccg
    19321 cgccgccgcg gtctgtccgg tgggcgtgat cctcagtaaa cgggtgggct tcgccgtgcc
    19381 gatcgggcag cggacctacg accgccggcc gatcagcgcg agcaccagcc ccgaaggcaa
    19441 ggccggtgcc ggcatcgagg aggctcgatg atggacatcg acatcgcaaa gatcgacgct
    19501 ggcggcgcgc cacgcaagct caaggtcgcc accgtctcgc tcgcgggctg cttcggctgc
    19561 cacatgtcct tcctcgacat cgacgagcgc ctgctgccgc tgctcgatct gatcgagttc
    19621 gaccgctcgc cgctcaccga catcaagcac tgcggtccct gcgacatcgg cctcatcgag
    19681 ggcggggtgt gcaacgccga gaacgtgcat gtgctgcgcg aattccgcgc caactgcaag
    19741 gtgctgatcg cgctcggcgc gtgcgcggtc aa
//
LOCUS       DAOQBS010000050        19652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1911_length_19652_cov_16.715518, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000050 DAOQBS010000000
VERSION     DAOQBS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 19652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19652
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1911_length_19652_cov_16.715518"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(351..426)
                     /locus_tag="PKN25_07510"
     tRNA            complement(351..426)
                     /locus_tag="PKN25_07510"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(391..393),aa:Lys,seq:ctt)
     gene            complement(598..1149)
                     /gene="ruvC"
                     /locus_tag="PKN25_07515"
     CDS             complement(598..1149)
                     /gene="ruvC"
                     /locus_tag="PKN25_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNV90782.1"
                     /translation="MSAAASTHVATRILGLDPGLRITGFGLVDQLGTQLRYVASGCIR
                     TRDGELPARLKTLLDGVREIVDTYRPDVVAVEKVFVNVNPQSTLLLGQARGAVICGAV
                     ACDLPVAEYTALQVKQSVVGYGKAHKEQVQHMVQRLLALDASPGPDAADALACAICHA
                     HGSRGLGALPGVGTRRRAGRILG"
     gene            complement(1146..1463)
                     /locus_tag="PKN25_07520"
     CDS             complement(1146..1463)
                     /locus_tag="PKN25_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNV90783.1"
                     /translation="MLMTTTSSLDGQPIRQYLGVVNGEAIIGANLFKDMFASIRNVVG
                     GRAGSYERTLADARRIAMEEMEEAAQKLGANAVIGIDVDYEVLGQDNGMLMVCVSGTA
                     VKI"
     gene            complement(1498..2379)
                     /locus_tag="PKN25_07525"
     CDS             complement(1498..2379)
                     /locus_tag="PKN25_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV90784.1"
                     /translation="MPTPPLLTRLTPLLFVLLWSTGFIGAKFGLPYAEPLTFLSSRYV
                     LVIALMSLLALAMRAPWPASPREALHIGVTGVLVQALYLGGVFMSIHRGLPAGISALV
                     VGMQPLLTAALAGALLGERVSARQWAGLALGFGGVALVVGSKANVDGVDGGALIHMLV
                     PALAALLGITAGTLYQKRFCPRFDLRTGSVMQFLPSLAITALIASQTETMRIEWTGDF
                     VFALGWLVLVLSIGAISLLNLLIRSGSAVNVASLFYLTPPTTALIAWAMFDEKLSALA
                     IAGMAIAVSGVWLARKG"
     gene            complement(2447..3172)
                     /locus_tag="PKN25_07530"
     CDS             complement(2447..3172)
                     /locus_tag="PKN25_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNV90785.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRGAAFSKLAKEITVAAKMGGGDPG
                     FNPRLRLAVDKAKGVNMPKDKIDNAIKKGTGELEGVSYEEIRYEGYGIGGSAVMVDCL
                     TDNKTRTVADVRHAFSKYGGNMGTDGCVSFQFKHCGQLLFAPGTSEDALMEAALEAGA
                     EDVVTNDDGSIEVITGPWEFTTIKEALEKAGFTAEFGEVTMKPENVIELAGDEAARMQ
                     KLLDALEGLDDVQEVYTSAEFDE"
     gene            3446..4963
                     /locus_tag="PKN25_07535"
     CDS             3446..4963
                     /locus_tag="PKN25_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HNV90786.1"
                     /translation="MADPMLIARAHDKDQFKDICLLPRLSNRHGLITGATGTGKTVTL
                     QKLAESFAHIGVPVFVADIKGDLSGIGAAGVRSDKLMQRLAAIGIDDYTPRANTVVFW
                     DVFGEQGHPVRATISDMGPLLIARLLNLNDTQTGVLTLVFKIADDNGMLLLDLKDLRA
                     MVQYVGENAKSFTTEYGNISSASIGAIQRNLLALEEQGGDVFFGEPMLDINDLMQTDA
                     DGRGVINVLAADKLYHSPKLYSTFLLWMLSELFEQLPEVGDPDKPKMVFFFDEAHLLF
                     NDAPKALIEKIEQVVRLIRSKGVGVYFVTQNPLDVPDTVLGQLGNRVQHALRAFTPRD
                     QKAVKTAADTMRPNPAFDAVAAITELGVGEALISFLDEKGRPQITERCNVIAPDSRLG
                     PLEPAERRAAIAGSVIYGHYEKAVDRESAYELLKASAATKAAVEAGRAAAKEVGGEDT
                     GSVVSDILFGATGPRGGKRDGLVQIAAKTVTRTIGSSLGRQIVRGILGSLLGGKR"
     gene            complement(5172..7076)
                     /locus_tag="PKN25_07540"
     CDS             complement(5172..7076)
                     /locus_tag="PKN25_07540"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV90787.1"
                     /translation="MKTLEHIPLNGELPSPKGVALAILELCRKDDATIAEVARIAQTD
                     PALASRLIRQANSAAHGGGRPVASVTEAIMRLGMGTVRNLALGFSLVDQYQNGPCGQF
                     DYQSFWSHSLLMGLAMQRFSNMTGVGSSEELFACGLLAQVGRLSLATVYPVEYGRLLD
                     GQDGAARSLVALEQEHLQTDHNELTAALLGEWGIPGALIEPIYHHEDPESSGFSRGSR
                     PYQLVHLFYLARRIADLGLAGESERNACTAELLLLGGRIGLDAEQLGATVDDLVQQWR
                     TWGELLRVPASALPSFAHMSAAHAMRDEKSDGAAALRVLLVDDDPTTRCMMEGVLSGA
                     LGHAVVCANNGREALALAVETQPQIVVTDWMMPGMDGLDLARALRATEWGQTMYLIML
                     TGHENEDEVSEAFEAGVDDFLVKPVNVRTLRARLRAARHYVQLLEAWERDRAQLKQFA
                     AELAISNRRLAHAAMTDLLTNLPNRRSGMNALAKAWAASSRFEQPLSVMMLDIDHFKR
                     INDSHGHAVGDTVLRDVAQAIQHSARKDDSMCRMGGEEFLVICPNTDLKAAVQAAERL
                     RRMVERLRIQAGETEIQTSVSIGVATREVGMTETDALVNAADRALYRAKQAGRNRSCV
                     VVQGQMRCFP"
     gene            7185..9161
                     /locus_tag="PKN25_07545"
     CDS             7185..9161
                     /locus_tag="PKN25_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV90788.1"
                     /translation="MKLAVLGLGVLLAFPPEAGAAQDDLTALPFEELLLRDFVSASRL
                     ARQVSDSPAAVAIVTADDIRAYGYRTLADVINGMRGLYTTDERTYHYMGGRSFGDVED
                     YAGRVMLLIDGYAVQDNLFDQAYIDESGLIDLELVDRVEYVPGTGSVTYGNNALLGIL
                     NVVTRRGRDFDGARVSAEISSRGASRQRATWGKHFNNGAEVLLSASTLDVDGRNLYFP
                     AYDTPATNFGVAEGLDGERNQRVFGKLSWSGWTVQAAWVEREKSVPTNPSAYTAFNTP
                     FPTRDESAFLGVRYETDLGLQLYSSSSLMLGRYAYWNQREYALDEDGEYDDGEKYGVR
                     DYHGAWWRFDQKFVGRWFVDHTLVFGAELRDDHRQSFDRRFLSPAGEVTDRDDGELSR
                     RTFSLYVADDYRLNQQWTLNLGVRHDDADDLDGNLSPRAALIWQQDPATTWKASYSEA
                     FKMPNANDRWTSDDTAVPEYVAATELVLQRQLAPHTRFTGSLYRYRRSDLPIENADGD
                     EVPEGSSRARGVETEIEHVWERGARARASVAWQRSRDVYGRDAVNSPDLLGKLAFTFL
                     LPGEALRAGLETQYLGPRLTRERRMLGGVTLSNLTLSTERDWHGLSASLSVRNLFDRD
                     YETVSGFDWRPGDVAQDGLRMDGRSVWLQVGYAL"
     gene            9158..9673
                     /locus_tag="PKN25_07550"
     CDS             9158..9673
                     /locus_tag="PKN25_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNV90789.1"
                     /translation="MSARNLFFGLALATSQGVLAQALPEFDLKATYAYNFAVLTQWPP
                     ATRASFNFCVYGDDGVAEAMRRLQGKSVHGRSVAVARLGALTAIRDCDLLYVGRDEAP
                     SLPHIAALLGDAPVLTVTDATQPQPVAVVIVPEGRRLAFEIDVERSRRAGLKPSAALL
                     GLARNLRQPLN"
     gene            complement(9666..11777)
                     /locus_tag="PKN25_07555"
     CDS             complement(9666..11777)
                     /locus_tag="PKN25_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV90790.1"
                     /translation="MSSTRILVADDDPTARLLMQAALGKSGFEVAVAEDGEEALQAFA
                     HATFDMVLLDVEMPRLDGYAVCRELRRRCGDALPIVMVTGMDDTASIEAAFEAGATDF
                     VAKPISWPLLGHRVRYVLRATRAATALRMANARNAAMLDAFPDTLLRLDAADRIVELR
                     AAPTGVPSCCMPAVGQTLAEAYPHEVATLLAQALDEARRSRQPSTVDFPLACRTGRPC
                     HQEARVSAIDDGEALCLLRDITERKEYETRIHHLAYFDSLTGLANRRSFLERLEREIR
                     RALQNKWRLAVLFLDLDRFKSINDTLGHAAGDALLQQAAERLRAITRPGDIVAHDTDR
                     MGTAEFARLGGDEFTVLLPTISRPDDALAIAERIRASICAPFAIEGQELCVTTSVGIA
                     VFPDDGEDAATLIKHADIAMYDAKANGRNGCRYYNPALTAEAVHRVELEAGLRRALAH
                     DELVLMYQPQVEVRDGSIATVEALVRWQTPDGRLVPPLEFIPFAEECGLIDAIGSWVL
                     RRACHDAAQWQRRGLDLRVAVNLSPVQFRASGLLDRIREALADSDLPAERLELEITES
                     ALMADDADTHATLAALRTLGIGIALDDFGTGYSSLSCLKRLPLDKLKIDRSFVSGLPD
                     DVDNHAIVRAILALARHLGFRITAEGVETAAQAELLGELEADSLQGYLFSRAVPAAEL
                     EALASRRWPPARGTQFRIDFS"
     gene            complement(11774..14539)
                     /locus_tag="PKN25_07560"
     CDS             complement(11774..14539)
                     /locus_tag="PKN25_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV90791.1"
                     /translation="MKMSHDEDDNRMTLGEKLRRVSQRALGAAVAMLAVAITLGSFLI
                     STLALESNTRVMARVLADNAAAGLMFLDHDAATRVLATLERLPDVRGAAIFDVSGAPF
                     ARYGKFIEQGRETVDFSAEHAHFSFETLHLTQPIVEGGNVVGVLDLDLDLRPLYLQVL
                     AHGSITVLAAAIALLLATHTLRRLNRAVLQPLSTLSALMERVAHTEDYSARAEPSRII
                     ELDSLASGFNRMLGQIAERDARLASHRDHLEDEVATRTEELLRAKEAAEAASQAKSDF
                     LATMSHEIRTPMNGVLGMTELLLDSGLNHEQRHFAEAVENSGRHLLGIINDILDFSKI
                     ESGHLELEAVEFELGDLLEDTLAMFAQPAEKKRLELIADLPPEVRRCVRGDPFRLRQI
                     VGNLANNAIKFTERGEVVVRARLLGEGTDGYRLRISVEDTGIGIPPHAQQKIFEQFSQ
                     LDGSTTRQFGGTGLGLAICRRLVGLMGGEIGVDSRPGAGACFHIDLCLPAGQSTAEAL
                     RCDVALERVHALVVDDNATNREILLRQLHGWGMQVECSADGAGALDRLARAATAGEGF
                     ELAVLDMHMPHMDGLQLATAIRANPALAGVRLVVLTSSYSSASVRERERAGILRCVHK
                     PIRQAELFEVLCSALRAVDAVAAATAVADEWRPTRGGRVLLAEDNPVNQQVGLAMLAK
                     LGIAVRVANDGEEAVKLAATEAFDLILMDCHMPVLDGYEASAAIRASEHAPRVPIVAL
                     TANVMEGNRERCLAAGMDDFLAKPYALEQLQSTLQRWMAATPASAPATPCDAATEAEA
                     TEVTEAAIDRTFIEQFRELDPDGGMALAARILRVYAESSAPPFAEIERATAAGDAAGL
                     RRAAHSLKSSAGNVGARRLATLLKDFESLGKDGRIDDARAGLAALRTEHARTLDAIHA
                     LIEEFGR"
     gene            complement(14536..15087)
                     /locus_tag="PKN25_07565"
     CDS             complement(14536..15087)
                     /locus_tag="PKN25_07565"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNV90792.1"
                     /translation="MLSLSIRPPRRRLRAFILGALLAGVANLAHAGDITAEEYRVKAS
                     FLFNFAAFTFWPEGGGETLSICVYGGDPFGSHLDALMDRKVGERSVRVRRVGSVDALV
                     DCQLVFVPRQMIGNLGRVLDGVDGQPVLTVADSPGALASGVVLNMDSSAGQIRFSVNL
                     AAARRQGLGLSSKLLNLATEIKR"
     gene            complement(15094..17079)
                     /locus_tag="PKN25_07570"
     CDS             complement(15094..17079)
                     /locus_tag="PKN25_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV90793.1"
                     /translation="MTGALALPFAAAAEAPPDLSLEQLIALPVESAAEYERVAVVGAS
                     KYEQTKDRVAAAVTAITRDEIRSFGWRTLDEALATLPGVHTTYDRQYSYVGVRGFGLA
                     GDYMTRVLVTIDGVRVNEPMYDGAPFGRMLPLDMDLVERIEFIPGPGGAIYGQNAMFG
                     VVNVITRKGAALDGGEFSASWQWPQATRKARASWGKRLDNELELMVSATVLRSDGDDL
                     WMDFGNGDSATVRGQDGERDQEFNLSASRGPWSFGLAHGDRRKDDPTGVFGTDAFASG
                     TFQGDRYTVAHLGYSATLTDTLSLSARAFAGDYRYESTLMYDGEGYEYPASAAWHGAE
                     LQLVSTAVAGHTLLLGTEYQRNQRIDQNIVHPDIGYLLRDRRDGYRAGLFVQDEWQIT
                     DAFASTLGLRADHNDLTGTEFSPRAGLIWNAGKHNTLKLLYGRAHRAPNAYQMYYDDA
                     YLIEDGTIGACQRANPSLGGEVVETTELVLDHIASANLHLRASLYRWKIDDLINLVAD
                     ADSVGQYQAVGSVESRGLELSADHTWRNGARLRGNLSFQHATGAAGERVENSPRMLGR
                     MNFSTPLANLPLRMGVEFAYDSKRRSPAGNTVDAYWRSNLHLVAERWIQGAEVSLSVM
                     NLFDEDYAHPSGGAPIHTMDRIAQDGRSLRVKFDYRF"
     gene            complement(17325..18158)
                     /gene="metF"
                     /locus_tag="PKN25_07575"
     CDS             complement(17325..18158)
                     /gene="metF"
                     /locus_tag="PKN25_07575"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNV90794.1"
                     /translation="MKPEISIEFFPPTTAEGAEKLHATREKLAALEPTFFSVTYGAGG
                     STRERTFATVKEIAAAGHEAAPHLSCVGSSRDSIREILQEYAAAGIRRIVALRGDLPS
                     GAGAAGEFRYASELVEFIRSETGDRFRIEVAAYPEWHPQAKSPKDDLAAFKRKVEAGA
                     DSAITQYFYNLDAYLNFVDAVRRMGVDVPIVPGIMPIGSFSKLARFSDACGAEIPRWM
                     RKTFESYGDDSDAIRAFGLDVVTRLCEKLLAAGAPGLHFYSMNMAGPTTEICRRLGLT
                     A"
     gene            complement(18155..19012)
                     /locus_tag="PKN25_07580"
     CDS             complement(18155..19012)
                     /locus_tag="PKN25_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNV90795.1"
                     /translation="MSMNSFHARQGKPRPAAQGKGPADRHGLLRVDQLLVAQGLAASR
                     TAARALIEAGRVSHEGQPVAKPALELPPDARLAVTPADSDRFVSRGALKLEGALARSG
                     LDVRGATCLDIGQSTGGFTDCLLQAGAARVVGVEVGHDQLHPRLRADGRCVTLEGVNA
                     RQLDAAALGGHYPPHGFDLLVCDASFISLALLLPQWAALLAPAGHVLALVKPQFEVGP
                     QGLGKGGIVRDPARYTEVEARLRAAADAAGLVILDWFDSPITGGDGNREFFFHAIRRP
                     GGDTHRSEP"
     gene            complement(19103..>19652)
                     /locus_tag="PKN25_07585"
     CDS             complement(19103..>19652)
                     /locus_tag="PKN25_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNV90796.1"
                     /translation="ALQAAMEGYRVVTMEYAADKADIFVTATGNYHVITHDHMAKMKD
                     QAIVCNIGHFDNEIDVASIEQYQWEEIKPQVDHVIFPDGKRIILLAKGRLVNLGCATG
                     HPSYVMSSSFANQTIAQIELFTRTADYPIGVYTLPKHLDEKVARLQLKKLNVQLTELT
                     DAQAAYIGVPKQGPYKSDAYRY"
BASE COUNT         2973 a         6542 c         7001 g         3136 t
ORIGIN      
        1 aagcatgagg gctcaacggg ggctacgtaa ggatggggtt cgtggagcgc ttacgcacaa
       61 cttgacgcac acaactgacc cgacaggccg cgcacgcttg accagcgcca gcatcaccac
      121 caagacgggc cccgcgccgg gtttcttttc tggcacgccg ccagggcaag agggtagcga
      181 aacgcgcccc agaccgaggc cggacatttt cgcggacatg ctggcgctgc tgctaggcgg
      241 aggtggccga gcgcgtggca tcgggaaggg ccgacgtttt ccattcctac ccaaacgcca
      301 aaaagcaaaa aggccaacct cgaaagattg gcctaagtgc ttgaatttgt tggtgggggc
      361 actaggactc gaacctagga ccaattgatt aagagtcaac tgctctacca actgagctat
      421 acccccgtgc cttgcggcgg atcggattgg cgcccgatcg aagagcgcgc atcatacgcg
      481 caggtgtggc gcagtgcaac agatttttcg tccggcgcag gcgcggaacc cggccaccgc
      541 gcttgcgcct tctggcactc ccctggaaag gccatgcttc ggaaggcccg gcagcgctca
      601 gcccaggatg cgccctgcgc ggcgacgcgt gccgacgccg gggagggcgc cgaggccgcg
      661 cgagccgtgg gcgtggcaga tcgcgcaggc gagcgcgtcg gcggcgtcgg ggccggggct
      721 ggcgtcgagg gcgagcaggc gctgcaccat gtgctgtacc tgttccttgt gcgccttgcc
      781 gtagccgacc accgactgct tgacctgcag cgcggtgtat tcggccaccg gcaggtcgca
      841 ggccaccgcg ccgcagatca ccgcgccgcg ggcctggccg agcagcaggg tggattgcgg
      901 attcacgttg acgaagacct tctcgaccgc gaccacatcc ggccggtagg tgtcgacgat
      961 ctcgcgcacg ccgtcgagca gggtcttgag gcgggcgggg agttctccgt cccgggtcct
     1021 gatgcagccg ctggcgacgt agcgcagctg ggtgccgagc tggtccacca ggccgaagcc
     1081 ggtgatgcgc agcccggggt cgaggccgag gatgcgggtg gcgacgtgcg tgcttgcggc
     1141 agcgctcaaa tcttcaccgc ggtgccgctc acgcacacca tcagcatgcc gttgtcctgg
     1201 ccgaggacct cgtagtcgac gtcgatgccg atcaccgcgt tggcgccgag cttctgcgcg
     1261 gcttcttcca tctcttccat ggcgatgcgg cgggcgtcgg ccagcgtgcg ctcgtagctg
     1321 ccggcgcggc cgccgaccac gttgcggatc gaggcgaaca tgtccttgaa caggttggca
     1381 ccgatgatgg cctcgccatt gaccacgccg aggtactggc ggatcggctg gccgtcgagg
     1441 ctcgaggtgg tggtcatcag catggcatgc tcctgcggag tgggaggagc cccgattcta
     1501 gcccttgcgc gccagccata cacccgagac ggcgatggcc atgccggcga tggcgagtgc
     1561 ggatagcttc tcgtcgaaca tcgcccaggc gatcagcgcc gtggtcggcg gcgtcaggta
     1621 gaacaggctc gccacgttca ccgcgctgcc gctgcggatc agcaggttga gcaggctgat
     1681 cgcgccgatc gacagcacca gcaccagcca gccgagcgcg aagacgaagt cgccggtcca
     1741 ctcgatccgc atcgtctcgg tctggctggc gatcagtgcg gtgatcgcca gactgggcag
     1801 gaactgcatc accgagccgg tacggaggtc gaagcgcggg cagaagcgct tctggtacag
     1861 ggtgccggcg gtgatgccga gcagcgcggc cagcgcgggg acgagcatgt ggatcagcgc
     1921 gccgccatcg acgccatcca cgttcgcctt gctgcccacc accagcgcga cgccgccgaa
     1981 gccgagcgcg agcccggccc actgccgcgc cgatacccgc tcgccgagca gcgcgccggc
     2041 cagtgcggcg gtgagcagcg gctgcatgcc caccaccagc gccgatatgc ccgccggcag
     2101 cccgcggtgg atcgacatga acacgccacc caggtacagc gcctgcacca gcacgccggt
     2161 gacgccgata tgcagcgcct cgcgcgggct ggccggccag ggcgcgcgca tcgccagcgc
     2221 gagcaggctc atcagagcga tcaccagcac gtagcggctg ctcaggaagg tgagtggctc
     2281 ggcgtagggc aggccgaact tggcgccgat gaagccggtg ctccacagca gcacgaacag
     2341 cagcggcgtc aggcgggtga gcaggggcgg agtcggcatc gcggtgggtc cgtgaaaaac
     2401 aaaaagcggc gccgggcgcc gctttcctgg gggcagtcgg gagagcttac tcgtcgaatt
     2461 cggcggaggt gtacacctcc tgcacgtcgt ccaggccttc gagcgcgtcg agcagcttct
     2521 gcatgcgcgc ggcctcgtcg ccggcgagct cgatgacgtt ctccggcttc atcgtgacct
     2581 cgccgaactc ggcggtgaag ccggcctttt ccagcgcttc cttgatggtg gtgaactccc
     2641 acgggcccgt gatgacctcg atcgagccgt cgtcgttggt gacgacgtct tcggcgccgg
     2701 cttcgagcgc ggcttccatc agcgcgtcct cgctggtgcc gggggcgaac aggagctggc
     2761 cgcagtgctt gaactggaag gacacgcagc cgtcggtgcc catgttgccg ccgtacttgg
     2821 agaaggcgtg gcgcacgtcg gccacggtgc gggtcttgtt gtcggtgagg cagtcgacca
     2881 tcaccgccga gccgccgatg ccgtagcctt cgtagcggat ctcctcgtag ctcacgccct
     2941 cgagctcgcc ggtgcccttc ttgatcgcgt tgtcgatctt gtccttgggc atgttgacgc
     3001 ccttggcctt gtcgaccgcc aggcgcaggc gcgggttgaa gccgggatca cctccgccca
     3061 tcttggcggc gacggtgatc tccttcgcca gcttggagaa tgccgcaccg cgcttttcgt
     3121 cctgacgacc cttgcggtgc tggatgttgg cccatttcga gtgacccgcc atattggaac
     3181 ctctgtgctg cccgagcgat tgaagaggcg cgcattatag cgcccgctcc gtgtgtgcgc
     3241 ctcgggtgca gcgggcgcca ccgcgacgta ggagcgacgc aagtcgcgat cggaggtttc
     3301 tgcggcgctg gcgcggattt catgcgctgc aatcgcgact tgcgtcgctc ccacgaagaa
     3361 ccaccgcgcc tgcgggggcc atgggaggcc gcgggcgggg ctgctaaact cggcgcattc
     3421 cgaaccgaat cccgaggagc atgaaatggc cgacccgatg ctgatcgcgc gcgcgcacga
     3481 caaggaccag ttcaaggaca tctgcctgct gccgcgcctg tccaaccgcc acggcctcat
     3541 caccggcgcc accggcaccg gcaagacggt gaccctgcag aagctcgccg aatccttcgc
     3601 ccatatcggc gtgccggtgt tcgtcgccga catcaagggt gacctctccg gcatcggcgc
     3661 cgcgggcgtc aggtccgaca agctgatgca gcgcctggcc gcaatcggca tcgacgacta
     3721 caccccgcgt gccaacacgg tggtgttctg ggacgtgttc ggcgagcagg gccatccggt
     3781 gcgcgccacc atctccgaca tgggcccgct cttgatcgcg cgcctgctca acctcaacga
     3841 cacccagacc ggcgtgctca cgctggtgtt caagatcgcc gacgacaacg gcatgctgct
     3901 gctcgacctc aaggacctgc gcgcgatggt ccagtacgtg ggcgagaacg cgaagagctt
     3961 caccaccgag tacggcaaca tctccagcgc ctcgatcggt gccatccagc gcaacctgct
     4021 ggcgctggaa gagcagggcg gcgacgtgtt cttcggcgag ccgatgctcg acatcaatga
     4081 cctgatgcag accgacgccg acggccgcgg cgtgatcaac gtgctggccg ccgacaagct
     4141 ctaccactcg cccaagctct actccacctt cctgctgtgg atgctgtccg agctcttcga
     4201 gcagctgccc gaggtcggcg accccgacaa gccgaagatg gtgttcttct tcgacgaggc
     4261 ccacctgctg ttcaacgacg cgcccaaggc gctgatcgag aagatcgagc aggtggtgcg
     4321 cctgatccgc tccaagggcg tgggcgtgta tttcgtcacc cagaacccgc tcgacgtgcc
     4381 cgacaccgtg ctcggccagc tcggcaaccg cgtccagcac gcgctgcgcg ccttcacccc
     4441 gcgcgaccag aaggcggtga agaccgccgc cgacaccatg cgccccaacc cggccttcga
     4501 cgccgtggcg gcgatcaccg agctcggcgt gggcgaggcg ctgatctcct tcctcgacga
     4561 aaaaggccgg ccgcagatca ccgagcgctg caacgtcatc gcgcccgatt cgcgcctcgg
     4621 cccgctcgag cccgccgagc gccgggcggc gatcgccggc tcggtgatct acggccacta
     4681 cgagaaggcg gtcgaccgcg aatccgccta cgagctgctc aaggccagcg ccgcgaccaa
     4741 ggccgcggtc gaggccggcc gcgcggcggc gaaggaggtc ggcggcgagg ataccggcag
     4801 tgtggtcagc gacatcctct tcggcgccac cggcccgcgt ggcggcaagc gcgacggact
     4861 ggtgcagatc gccgccaaga cggtcacgcg caccatcggc agcagcctgg ggcggcagat
     4921 cgtgcgcggg atattgggct cgctgctggg cggcaagcgc tgaaggggac gggagggaga
     4981 ttgcatccct tccagcggcc gcgcgggcaa gcgccgcgcg ccgcggtgtc cattaccgga
     5041 tcgcgacttg cgtcgctcct acaggcgcct tcgggagcgc ggcggtctcg taggagcgac
     5101 gcaagtcgcg atcccgcgct tcagcgctgc cgcggccgat tcgcgccgcg cgcaggccgg
     5161 ttcccgacgc ctcacggaaa gcaccgcatc tgcccctgca ccaccacgca gctgcggttg
     5221 cggccggctt gcttggcgcg gtagagggcg cgatcggcgg cattgacgag cgcgtcggtc
     5281 tcggtcatgc cgacctcgcg ggtggccacg ccgatgctca ccgaggtctg tatttccgtt
     5341 tcgccggcct ggatgcgcag gcgctcgacc atgcggcgca ggcgctcggc ggcctggacc
     5401 gccgccttga ggtcggtgtt cgggcagatc acgaggaatt cctcgccacc catgcggcac
     5461 atgctgtcgt ccttgcgcgc cgagtgctgg atggcctggg cgacgtcgcg cagcacggtg
     5521 tcgcccaccg cgtggccgtg gctgtcgttg atgcgcttga agtggtcgat gtcgagcatc
     5581 atcaccgaca gcggctgttc gaagcggctg gaggcggccc aggccttggc gagcgcgttc
     5641 atgcccgagc ggcggttggg caggttggtg agcaggtcgg tcatcgccgc gtgggcgagg
     5701 cggcggttgc tgatcgccag ctcggccgcg aactgcttga gctgggcacg gtcgcgctcc
     5761 cacgcctcga gcagctgcac gtagtgccgg gcggcgcgca gacgcgcgcg cagggtgcgc
     5821 acgttgaccg gcttcaccag gaaatcgtcc accccggcct cgaaggcctc gctcacctcg
     5881 tcctcgtttt catgcccggt gagcatgatc aggtacatgg tctgccccca ctcggtcgcg
     5941 cgcagcgcac gcgcgaggtc cagcccgtcc atgcccggca tcatccagtc ggtgacgacg
     6001 atctgcggct gggtctcgac cgccagcgca agcgcctcgc gaccgttgtt ggcgcacacc
     6061 accgcgtggc cgagcgcccc ggacagcacg ccctccatca tgcagcgcgt ggtggggtcg
     6121 tcgtcgacca gcagcacgcg cagcgccgcc gcgccatcgc tcttctcgtc gcgcatcgcg
     6181 tgcgccgccg acatgtgcgc gaacgagggc agcgccgagg ccggcacgcg cagcagctcg
     6241 ccccaagtgc gccactgctg caccaggtcg tccaccgtgg cgccgagctg ttcggcgtcg
     6301 aggccgatcc tgcccccgag cagcagcaac tcggcggtgc aggcgttgcg ttcgctttcg
     6361 ccggcgagac cgaggtcggc gatgcggcgc gcaaggtaga agagatgtac cagctggtag
     6421 ggccgtgagc cgcgcgagaa gccggagctt tcagggtcct cgtggtggta gatcggctcg
     6481 atcagcgcgc cggggatgcc ccactcgccg agcagcgcgg cggtgagctc gttgtgatcg
     6541 gtctgcaggt gctcctgctc gagcgccacc aggctgcgcg cggcgccgtc ctggccgtcg
     6601 agcaggcggc cgtactcgac cggatagacg gtggcgagcg agagccggcc cacctgggcg
     6661 agcaggccgc aggcgaagag ctcctcgctc gagccgaccc ccgtcatgtt gctgaacctc
     6721 tgcatcgcca gccccatcag cagcgaatgc gaccagaagc tttggtagtc gaactgtccg
     6781 cagggcccgt tctggtactg gtccaccagc gagaagccga gcgcgaggtt gcgcaccgtg
     6841 cccatgccca ggcgcatgat cgcctcggtg accgaggcca ccggccgtcc gccaccgtgc
     6901 gccgccgagt tggcctggcg aatcaggcgg ctcgccagcg ccgggtcggt ctgcgcgatg
     6961 cgcgccactt cggcgatcgt ggcgtcgtcc ttgcggcaca gttcgaggat cgccagcgcg
     7021 actcctttcg gactggggag ttcgccgttc aggggaatgt gttcgagggt tttcatcggg
     7081 ggtacggccg ggcgcgggac cagcctgata agatacccga ttcgggcgtc agccaatatg
     7141 caggaatgga gtggggagct gaaaaggtcg tggatcctcg tcggttgaag ctggccgtgc
     7201 tgggcctcgg cgtgctgctc gcgttcccgc ccgaggctgg cgccgcgcag gacgacctga
     7261 ccgcgctgcc cttcgaggag ttgctgctgc gcgatttcgt gtccgcgtcg cggctcgcac
     7321 gccaggtgag cgactcgccc gcggcggtgg cgatcgtcac cgccgacgac atccgcgcct
     7381 acggctaccg cacgctggcc gacgtcatca acggcatgcg cggcctctac accaccgacg
     7441 agcgcaccta ccactacatg ggcgggcgca gcttcggcga cgtcgaggac tacgccggcc
     7501 gcgtgatgct gctgatcgac ggctacgcgg tgcaggacaa cctcttcgac caggcctaca
     7561 tcgacgaatc cggcctgatc gacctggaac tggtcgatcg cgtcgagtac gtgccgggaa
     7621 ccggctcggt gacctacggc aacaacgcgc tgctcggcat cctcaatgtc gtcacccggc
     7681 gcgggcgcga cttcgacggt gcgcgcgtgt cggcggagat ctccagccgc ggcgccagcc
     7741 gccagcgcgc cacctggggc aagcatttca acaacggcgc cgaggtcctg ctctcggcct
     7801 cgacgctcga cgtcgatggc cgcaacctgt acttccccgc ctacgacacg cccgcgacca
     7861 acttcggcgt ggccgagggg ctcgacggcg agcgtaacca gcgcgtgttc ggaaagctgt
     7921 cgtggtcggg ctggaccgtt caggcggcgt gggtggagcg cgagaagagc gtacccacga
     7981 acccctccgc atacaccgcc ttcaacaccc cgttcccgac gcgcgacgag agcgccttcc
     8041 tcggggtgcg ctacgagacc gacctcggtc tgcagctgta ttcctcgtcc agtctgatgt
     8101 taggacgcta cgcctactgg aaccagcgcg aatacgccct cgacgaggac ggcgagtacg
     8161 acgacggcga gaagtacggc gtgcgcgact accacggcgc gtggtggcgc ttcgaccaga
     8221 agttcgtcgg gcgctggttc gtcgaccaca cgctggtgtt cggcgcggag ctgcgcgacg
     8281 accaccgcca gtccttcgac cgccgcttcc tctcgcccgc cggcgaagtc acggatcgtg
     8341 acgacggcga gctttcgcgc cgcaccttca gtctctacgt cgccgacgac taccggctga
     8401 accagcagtg gacgctcaac ctgggcgtgc gtcacgacga cgccgacgat ctcgacggca
     8461 acctcagccc gcgtgccgcg ttgatctggc agcaggatcc ggcgacgacc tggaaggctt
     8521 cgtacagcga ggccttcaag atgcccaacg ccaacgaccg ctggacgtcc gacgacacgg
     8581 ccgtccccga gtacgtcgcc gccaccgagc tcgtgctgca gcgccagctc gcgccgcaca
     8641 cgcgcttcac cggctccctg taccgctacc ggcgcagcga cctgccgatc gagaacgcgg
     8701 acggggacga ggttcccgag ggaagcagcc gcgcgcgcgg cgtcgagacc gagatcgagc
     8761 atgtctggga gcgcggggcg cgtgcgcgcg ccagcgtggc ctggcagcgc tcgcgcgatg
     8821 tgtacgggcg cgacgcggtc aactcgcccg acctgctcgg caagctcgcc ttcaccttcc
     8881 tgctgccggg cgaggcgctg cgtgccggcc tcgagacgca atatctcggc ccgcgcctga
     8941 cccgcgagcg gcgcatgctg ggcggggtga cgctttccaa tctgaccctg tccaccgagc
     9001 gcgactggca tggcctgtcg gcctcgctga gcgtgcgcaa cctgttcgat cgtgactacg
     9061 agaccgtgtc gggcttcgac tggcggcccg gtgacgtggc acaggacggc ctgcgcatgg
     9121 acgggcgcag cgtctggctg caggtcgggt acgcgctatg agcgcacgca acctgttttt
     9181 cgggctcgcg ctggcgactt cgcagggcgt gctcgcacag gcgttgcccg agttcgacct
     9241 caaggccacc tacgcctaca acttcgccgt gctcacgcag tggccgccgg cgacgcgcgc
     9301 gagcttcaac ttctgcgtct atggggacga cggcgtggcc gaggcgatgc gccggctgca
     9361 gggcaagtcc gtgcatgggc gcagcgtggc ggtggcgcgg cttggtgcgc tgaccgcgat
     9421 ccgtgactgc gacctgcttt acgtcggcag ggacgaggcg cccagcctgc cacacatcgc
     9481 agccttgctc ggcgatgcgc cggtgctgac cgtgaccgac gcaacgcagc cgcaaccggt
     9541 cgcggtggtg atcgtgcccg agggccggcg cctggccttc gagatcgatg tcgagcgcag
     9601 ccgccgtgcc gggctcaagc ccagcgcggc gctgctcggg ctcgcgcgca acctgcgcca
     9661 gccgctcaac tgaagtcgat gcggaactgg gtgccgcgcg ccggcggcca cctgcgcgag
     9721 gccagcgctt cgagctccgc ggccggcacc gcgcggctga acaggtagcc ctgcaggctg
     9781 tcggcctcga gctcgccgag cagctcggcc tgcgccgcgg tctcgacgcc ctcggccgtg
     9841 atgcggaagc ccaggtgacg tgcgagcgcg aggatcgcgc gcacgatcgc gtggttgtcg
     9901 acgtcgtcgg gcaggccgct gacgaagctg cggtcgatct tcagcttgtc cagcggcagg
     9961 cgcttgaggc agctcagtga cgagtacccg gtgccgaagt cgtcgagtgc gatgccgatc
    10021 ccgagcgtgc gcagtgcggc cagcgtggca tgggtgtcgg catcgtcggc catcagcgca
    10081 ctctcggtga tctcgagctc cagccgttcg gcgggcaggt cactgtcggc gagggcctcg
    10141 cggatcctgt cgagcagccc gctggcgcgg aactgcaccg gcgacagatt gaccgcgacg
    10201 cgcaggtcga gcccgcggcg ctgccattgt gctgcgtcat ggcaggcgcg acgcagcacc
    10261 caggagccga tggcgtcgat caggccgcat tcctcggcga aggggatgaa ctcgagcggc
    10321 ggcaccagcc tgccgtcggg cgtctgccag cgcaccaggg cctcgacggt ggcgatactg
    10381 ccgtcgcgca cctccacctg cggctggtac atcagcacga gctcgtcgtg ggcgagcgcg
    10441 cggcgaagcc cggcctcgag ttccacgcga tgcaccgcct cggcggtcag cgcagggttg
    10501 tagtagcggc agccgttgcg tccgttggcc ttggcgtcgt acatcgcgat gtcggcgtgc
    10561 ttgatcagcg tggcggcgtc ctcgccgtcg tccgggaaga cggcgatgcc gacgctggtg
    10621 gtgacgcaga gctcctggcc ctcgatcgcg aatggagcgc atatgctggc gcggatgcgc
    10681 tcggcgatcg ccagcgcgtc gtccggtcgg gagatcgtcg gcagcagcac ggtgaattcg
    10741 tcgccgccga ggcgagcgaa ttcggccgtg cccatgcgat cagtgtcgtg cgccacgatg
    10801 tcgcccgggc gggtgatcgc acgcaggcgc tcggcggcct gctgcagcag ggcgtcccct
    10861 gcagcgtggc cgagggtgtc gttgatgctc ttgaaacggt ccagatcaag gaagagcacc
    10921 gccaggcgcc acttgttctg aagggcgcgg cggatctcgc gctcgagccg ctccaggaag
    10981 ctgcgccggt tcgccagccc ggtcaggctg tcgaaatagg cgaggtggtg gatgcgggtc
    11041 tcatactcct tgcgctcggt gatgtcgcgc agcaggcaga gggcctcgcc gtcgtcgatt
    11101 gcgctcaccc gtgcttcctg gtggcagggg cgtccggtcc gacacgcgag aggaaaatcc
    11161 accgtgctcg gctgccggct gcggcgggcc tcgtcgagcg cttgcgcgag cagcgtcgcc
    11221 acctcgtgcg gataggcttc ggccagggtc tggccgaccg ccggcatgca gcagctgggc
    11281 acgccggtcg gggctgcgcg cagctcgacg atgcggtcgg ccgcgtcgag gcgcagcagg
    11341 gtgtcgggga aggcgtcgag catggccgcg ttgcgcgcgt tggccatgcg cagcgcggtg
    11401 gcggcgcggg tggcgcgcag cacgtagcgg acgcggtggc caagaagcgg ccagctgatc
    11461 ggcttggcga cgaagtcggt cgcgccggcc tcgaaggcgg cctcgatcga tgcggtgtcg
    11521 tccatgccgg tgaccatcac gatcggcagc gcgtcgccac agcggcggcg cagctcgcgg
    11581 cagaccgcgt agccgtccag gcgcggcatt tccacgtcga gcagcaccat gtcgaaggtg
    11641 gcgtgcgcga aggcctgcaa ggcctcctcg ccatcctcgg cgacggcgac ctcgaagccg
    11701 ctcttgccga gcgcggcttg catcagcagg cgtgcggtcg gatcgtcgtc ggcgaccagg
    11761 atgcgagtcg agctcatcga ccgaactcct cgatcagggc atggatggcg tcgagcgtgc
    11821 gcgcatgctc ggtgcgcagc gcggcgaggc cggcgcgcgc gtcgtcgatc cggccgtcct
    11881 tgccgaggct ctcgaagtcc ttgagcaggg tcgcgaggcg tctggcgccg acgttccccg
    11941 cgctcgactt gaggctgtgc gcggcgcgac gcagtcccgc ggcgtcgccg gcggcggtgg
    12001 cgcgctcgat ctcggcgaag gggggggcgc tgctctcggc atagacgcgg aggatgcgcg
    12061 cggcgagtgc catgccgccg tcggggtcga gctcgcggaa ctgctcgatg aaggtccgat
    12121 cgatcgccgc ctctgtcacc tcggtcgcct cggcttcggt cgcggcgtcg caaggcgtcg
    12181 ccggcgcgct cgcgggcgtt gccgccatcc agcgctgcag ggtggattgg agctgctcga
    12241 gcgcgtaggg cttggcgaga aaatcgtcca tgccggccgc caggcagcgt tcgcggttgc
    12301 cttccatgac gttggcggtg agcgcgacga tcggtacgcg cggtgcgtgc tccgatgcgc
    12361 ggattgccgc gctcgcctcg tagccgtcca gcaccggcat gtggcagtcc atcaggatca
    12421 ggtcgaaggc ttcggtcgca gccagcttca ctgcctcttc gccgtcgttg gcgacacgaa
    12481 ccgcgatgcc gagcttggcg agcattgcga ggccgacctg ctggttcacc gggttgtcct
    12541 cggccagcag cacgcgcccg ccgcgcgtcg gtcgccactc gtccgcaact gcggtggccg
    12601 ctgccactgc gtccacagcg cgcaatgcgc tgcatagaac ctcgaacagc tcggcctggc
    12661 ggatcggctt gtgcacgcag cgcaggatgc cggcgcgttc gcgctcgcgc acgctggcgc
    12721 tggagtagct cgatgtcagc accaccaggc gcacgccggc gagcgccggg ttggcgcgga
    12781 tcgcggtggc cagctgcaag ccgtccatgt gcggcatgtg catgtcgagt acggcgagct
    12841 cgaaaccctc gccggccgtg gcggcgcgtg cgagccgatc cagcgcgccc gcgccgtcgg
    12901 cagagcattc cacctgcatg ccccagccgt ggagctggcg cagcaggatc tcgcggttgg
    12961 tggcattgtc atccacgacg agggcgtgca cacgctcgag cgcgacgtcg cagcgcaggg
    13021 cttcggcggt gctttgcccc gccggcaggc acaggtcgat atggaagcag gcgcccgctc
    13081 cgggccggct gtcgacaccg atctcgccgc ccatcaggcc caccaggcgg cggcagatcg
    13141 ccaggccgag gccggtgccg ccaaactggc gggtggtgga gccgtcgagc tgggagaact
    13201 gctcgaagat cttctgctgc gcatggggcg ggatgccgat cccggtgtcc tcgacgctga
    13261 tgcgcaggcg atagccgtca gtgccctcgc cgagcagacg ggcacgcacc acgacctcgc
    13321 cacgctcggt gaacttgatc gcattgttgg ccaggttgcc gacgatctgg cgcaggcgga
    13381 aggggtcgcc gcgcacgcag cgccgcacct cggggggcag gtcggcgatc agctccaggc
    13441 gcttcttctc ggctggctgg gcgaacatcg ccagcgtgtc ctcgagcagg tcgccgagct
    13501 cgaactccac cgcttcgagc tcgaggtggc cggattcgat cttcgagaag tcgaggatgt
    13561 cgttgatgat gcccagcagg tggcgaccgg agttctcgac cgcctcggcg aagtggcgtt
    13621 gctcgtggtt gaggccgctg tcgaggagca gctcggtcat gccgagcacc ccgttcatcg
    13681 gcgtgcggat ctcgtggctc atggtcgcga ggaagtcgct cttggcctgg ctggcagcct
    13741 ctgcggcctc cttggcgcgc aggagctcct cggtgcgtgt ggcgacctcg tcctcgaggt
    13801 ggtcgcgatg gcttgccagg cgtgcatcgc gctcggcgat ctggccgagc atgcggttga
    13861 agccactggc gagggaatcg agctcgatga tacggctggg ttcggcgcgc gcgctgtagt
    13921 cctcggtgtg ggcgacgcgc tccatcagcg cggacagggt cgacagtggc tgcaacaccg
    13981 cgcggttgag ccggcgcagc gtgtgggtgg cgagcagcag cgcgatcgcc gcggcgagca
    14041 ccgtaatcga gccgtgggcg agcacctgga ggtagagcgg acgcagatcg aggtcgaggt
    14101 cgagcacccc gaccacgttc ccgccctcga cgatcggctg ggtcaggtgc agtgtctcga
    14161 agctgaagtg cgcgtgctcg gccgaaaagt ccaccgtctc ccggccttgt tcgatgaact
    14221 tgccgtaacg cgcaaaaggg gctccggata catcgaagat cgctgcgccg cgtacgtccg
    14281 gcaggcgctc cagggtcgcg agcacgcgcg tggcggcgtc gtggtcgagg aacatcaggc
    14341 cggcggcggc attgtcggca agcacccgcg ccatcacccg agtattgctc tcgagcgcga
    14401 gcgtgctgat caagaagcta ccgagggtga tcgccaccgc gagcatcgcc accgcggcgc
    14461 cgagcgcgcg ctggcttacg cggcgcagct tttcgccgag ggtcatgcgg ttgtcgtctt
    14521 cgtcgtggga catcttcatc gcttgatttc ggtggccaga ttgaggagct tcgagctgag
    14581 cccgagcccc tggcggcggg ccgccgcgag gttgacagaa aagcggatct gacctgcgct
    14641 gctgtccatg ttcaacacca cgccgctcgc cagggcgccc gggctgtcgg ccacggtgag
    14701 gacgggctgg ccgtccacgc cgtcgagcac ccggcccagg ttgccgatca tctgtcgggg
    14761 gacgaacacg agctggcagt ccacgagggc gtcgacgctg cccacacgcc gtacgcgcac
    14821 gctgcgttcg cccaccttgc ggtccatgag cgcgtcgaga tgcgagccga acgggtcgcc
    14881 accatagacg cagatcgaca gggtttcacc tccaccctcc ggccagaagg tgaaggcggc
    14941 gaagttgaac aggaaggatg ccttgaccct gtactcttcg gcggtgatgt cgcccgcatg
    15001 tgcgaggttc gccacgccag ccagcagcgc cccgaggatg aacgctcgca gccggcgtcg
    15061 tggcgggcgg atggaaagtg agagcatgct gggtcagaag cggtagtcga acttgacccg
    15121 caggctgcgg ccgtcctggg cgatgcggtc catggtgtgg atgggcgcgc cgccactcgg
    15181 atgggcgtag tcttcgtcga acaggttcat cacactcagt gacacctcgg cgccctggat
    15241 ccagcgctcg gccaccaggt gcaggttcga acgccagtac gcgtcgaccg tgttgcccgc
    15301 cgggctgcgg cgcttgctgt cgtaggcgaa ctccacgccc atgcgcagtg gcagatttgc
    15361 cagcggagtg gagaagttca tccggccgag catgcgcggc gagttctcca cgcgctcgcc
    15421 cgccgcgccg gtggcgtgct ggaaggacag gttgccgcgc aggcgggcgc cgttgcgcca
    15481 ggtgtggtcg gcggagagct ccagaccacg gctctcgacg ctgccgacgg cctgatattg
    15541 gccgacgcta tcggcgtcgg cgacaagatt gatcaggtcg tcgatcttcc agcggtagag
    15601 ggaggcgcgc aggtgcaggt tggccgaggc gatatggtcg agcaccagct cggtggtttc
    15661 gaccacttcg ccacccaggc tcgggttggc gcgttggcag gccccgatcg tgccatcctc
    15721 gatgaggtag gcatcgtcgt agtacatctg gtaggcgttg ggcgcgcggt gggcacgacc
    15781 gtacagcagc ttgagcgtgt tgtgcttgcc ggcattccag atcaggccag cgcgcgggct
    15841 gaactcggtg ccggtgaggt cattgtggtc ggcgcgcagg ccgagcgtgc tggcgaaagc
    15901 gtcggtgatc tgccattcgt cctgcacgaa gagtcccgcg cggtaaccgt cacggcggtc
    15961 gcgcaggagg tagccgatgt ccggatggac gatgttctgg tcgatgcgct ggttgcgctg
    16021 gtattccgta cccaacagga gcgtatggcc ggccacggcg gtcgacacga gctgcagctc
    16081 ggcgccgtgc caggcggcgg aggcggggta ctcgtagccc tcgccgtcat acatcagggt
    16141 gctctcgtag cggtagtcgc cggcgaaggc gcgcgccgac agcgacaagg tgtcggtgag
    16201 cgtggcactg tagccgaggt gggcgacggt gtagcggtcg ccctggaagg tgcccgaggc
    16261 gaaggcgtcg gtgccgaaca cgccggtggg gtcgtccttg cggcggtcgc cgtgcgcgag
    16321 cccgaaggac cacgggccgc ggctcgcgct gaggttgaac tcctggtcgc gctcgccgtc
    16381 ctggccgcgc accgtagcgc tgtcgccatt gccgaagtcc atccacaggt cgtcgccgtc
    16441 gctgcgcagg acggtggccg agaccatcag ctcgagctcg ttgtcgaggc gcttgcccca
    16501 gctcgcccgc gccttgcgcg tcgcctgcgg ccactgccac gaagccgaga actctcctcc
    16561 gtcgagtgcc gcacccttgc gggtgatcac attgacgacg ccgaacatgg cgttctggcc
    16621 atagatcgcg ccgcccgggc cggggatgaa ttcgatgcgc tcgaccaggt ccatgtcgag
    16681 cggcagcatg cgaccgaaag gggcgccgtc gtacatcggc tcgttgacgc gcacgccgtc
    16741 gatggtgacc agcacgcgcg tcatgtagtc gccggcgaga ccgaagccgc gcacgccgac
    16801 gtaggaatac tggcggtcgt aggtggtatg cacgccgggg agggtggcga gcgcctcgtc
    16861 gagcgtgcgc cagccgaagc tgcggatctc gtcgcgggtg atcgcggtga cggcggcggc
    16921 aacgcggtcc ttcgtctgct cgtattttga ggcgccgacc accgctaccc gctcgtactc
    16981 tgccgcgctc tccaccggaa gcgcaatcaa ctgctccagg ctcagatcag ggggcgcctc
    17041 ggccgccgcg gcgaagggca gcgcgagggc gccggtcaac agcagccggg ccagttggcg
    17101 ccgggcagat atgcagaggt ccgcgccagc cacgcaccga tcaatgcctt gggcaggacc
    17161 ggaggagaaa ggcgcgaaat acatggtggc ggatcctggc cgccagcacg aggcgtgggg
    17221 cggaagaaga tgtgctgatc gaaggactat acggcatacg ccacaggggc ttgagccgca
    17281 tacgtcacac ccaccttacg atcccgtcca ggattgccgg gagatcaggc agtcagcccc
    17341 agccgccggc agatctcggt ggtcgggccg gccatgttca tgctgtagaa atgcaggccg
    17401 ggcgcgcccg cggcgagcag cttctcgcac agccgggtga ccacgtcgag gccgaaggcg
    17461 cggatcgcgt cgctgtcgtc gccgtagctc tcgaaggtct tgcgcatcca gcgcgggatc
    17521 tcggcgccgc aggcatccga gaagcgcgcc agcttgctga agctgccgat cggcatgatg
    17581 ccgggcacga tcggcacgtc gacgcccatc ctgcgcaccg cgtcgacgaa gttcaggtag
    17641 gcgtcgaggt tgtagaaata ctgcgtgatc gccgagtcgg cgccggcctc gaccttgcgc
    17701 ttgaaggcgg cgaggtcgtc cttcgggctc ttcgcctgcg ggtgccactc ggggtaggcg
    17761 gcgacctcga tgcggaagcg gtcgcccgtc tcgctgcgga tgaactcgac gagctcgctg
    17821 gcgtagcgga actcgcccgc cgcgccggcg cccgagggca gatcgccgcg cagcgcgacg
    17881 atgcggcgga tgccggcggc ggcgtattcc tgcaggatct cgcggatgct gtcgcgcgac
    17941 gagcccacgc aggacaggtg tggcgcggcc tcgtggccgg cggcggcgat ctccttcacg
    18001 gtggcgaagg tgcgctcgcg ggtggagccg ccggcgccgt aggtcaccga gaagaaggtc
    18061 ggctcgagcg cggcgagctt ctcccgggtg gcatgcagct tctcggcgcc ctcggccgtg
    18121 gtgggcggga agaactcgat ggagatttcg ggtttcatgg ttcgcttcgg tgggtgtcgc
    18181 cgcccggcct gcggatggcg tggaagaaga attcgcggtt gccgtcgccg ccggtgatcg
    18241 gcgagtcgaa ccagtcgagg atgacgaggc cggcggcgtc cgctgcggcg cgcaggcggg
    18301 cctcgacctc ggtgtagcgc gcggggtcgc gcacgatgcc gcccttgccc aggccttgcg
    18361 ggccgacctc gaactgcggc ttgaccagcg ccagcacgtg gccggcgggt gcgagcagcg
    18421 cggcccactg cggcagcagc agggcgagcg agatgaagct ggcgtcgcac accagcaggt
    18481 cgaagccgtg cggcgggtag tgtccgccga gcgcggcggc gtcgagctgg cgggcgttga
    18541 cgccctccag ggtgacgcag cgcccatcgg cgcgcaggcg cggatggagc tggtcgtggc
    18601 cgacctccac cccgaccacc ctcgctgcgc cggcctgcaa caggcagtcg gtgaagccgc
    18661 cggtggattg gccgatgtcg aggcaggtgg cgccgcgtac gtccagcccg ctgcgcgcga
    18721 gcgcgccttc gagcttgagc gcgccgcgcg agacgaagcg gtcgctgtcg gcgggggtga
    18781 cggcgagccg tgcgtcgggc ggcaattcca gcgccggctt ggcgaccggc tggccctcgt
    18841 ggctcacccg cccggcctcg atcagcgcgc gcgccgcggt gcgcgaggcc gccagcccct
    18901 gcgccaccag cagctggtcg acgcgcagca ggccgtggcg gtccgccggg cccttgccct
    18961 gcgcggccgg ccgcggcttg ccctggcggg cgtggaagga gttcatgctc atgggcgtga
    19021 ggagtcaggg ggtgaggcgt gaaaagcctt tgtacaggag cgccggaagg cgctcctgcc
    19081 gggggctgcg ccgcccccgg gctcagtagc ggtacgcgtc cgacttgtac gggccttgct
    19141 tgggcacgcc gatgtaggcg gcctgcgcgt cggtcagctc ggtgagttgc acgttgagct
    19201 tcttcagctg caggcgggcg accttctcgt cgaggtgctt gggcagggtg tacacgccga
    19261 tcgggtagtc ggccgtgcgg gtgaacagct cgatctgtgc gatcgtctgg ttggcgaacg
    19321 agctcgacat cacgtagctc gggtggccgg tggcgcagcc gaggttcacc aggcggccct
    19381 tggcgagcag gatgatcctc ttgccgtcgg ggaagatcac gtgatcgacc tgcggcttga
    19441 tctcctccca ctggtactgc tcgatgctgg cgacgtcgat ctcgttgtcg aagtggccga
    19501 tgttgcagac gatggcctgg tccttcatct tcgccatgtg gtcgtgggtg atcacgtggt
    19561 agttgccggt cgcggtgacg aagatgtcgg ccttgtcggc ggcgtattcc atcgtgacca
    19621 cgcggtagcc ttccatcgcc gcctgcagcg cg
//
LOCUS       DAOQBS010000051        19260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1978_length_19260_cov_18.337204, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000051 DAOQBS010000000
VERSION     DAOQBS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 19260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19260
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1978_length_19260_cov_18.337204"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKN25_07590"
     CDS             complement(<1..296)
                     /locus_tag="PKN25_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNV90797.1"
                     /translation="MSDLALQGGSDPVHPVDEKLPGGRLAALGMQHVLVMYAGAVAVP
                     LIVGRALGFGPEQVALLISADLFCCGLVTLIQSLGFGKYFGIKLPVMMGVTFAAV"
     gene            complement(583..1098)
                     /locus_tag="PKN25_07595"
     CDS             complement(583..1098)
                     /locus_tag="PKN25_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmiS/UreI family transporter"
                     /protein_id="HNV90798.1"
                     /translation="MLLGLSLFYVGAVLILNGLWMLGRIGDREITIINLCTGGLTLLV
                     CLRLALGADADAASIRAAAFSLLFSFTYLWVAWNRLTGADGRGLGWFSLFVAITALPI
                     AADTLRAADSTWDWWLGLSWAAWAVLWLMFFLLLALHRPIARATAWMAIVQGMGTAWL
                     PGYLLLTGALY"
     gene            1400..2299
                     /locus_tag="PKN25_07600"
     CDS             1400..2299
                     /locus_tag="PKN25_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNV90799.1"
                     /translation="MSASGSPRLALSALALLSLIWGYNWVVMKQVIQYVDPFDFSAIR
                     TALGAATLFVVLIALRRPLGLQQVREVALLGVLQTGAFTALIQWALVAGGAGKTAVLV
                     YTMPFWVVPMAWFVLGERVRGMQWVANAIAGCGLVLVLEPWAMGGSAFSNLLAVAGGV
                     TWAAASVQAKRMRRAHDFDLLSLTAWQMLFGAVALCVLALLHPSRPIDPTPYFWGALV
                     FNAVFATGLAWLLWLFVLQRLSAGVAGLSALGIPMVGALAGWIELGERPTAVEFAGMA
                     LIVGALALTSLWSLRQARRASPR"
     gene            complement(2318..4456)
                     /locus_tag="PKN25_07605"
     CDS             complement(2318..4456)
                     /locus_tag="PKN25_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV90800.1"
                     /translation="MGETVNLLIVEDNESDAALVVRQLERAGYTVATARRVDRAAELG
                     AALEACAWSAVLCDYALPGFSPEEALAQVQASGLDLPFVVVSASLADEEAGRLMALGA
                     HDYVHKHDLARLAPALARGLAEARGRRARRHAEDGLRAREAHLRIWGEVVEGLHEGIV
                     VTNLAGDILDCNPAFERITGHARAEVLGLNPRVLKSGRHDAAFYAGMWDALVTRGSWV
                     GEVWNRRKSGEIYPERLALRAVRGADGLPTHYVGMFSDISREKQDQARLDYLTHHDPL
                     TGFANRQLFMERLQQAVERAAAGEGKAAVLLLDLDRFRRVNESLGQDAGDRLLQVMAE
                     RIAGCLKRRSSLARLGSDEFLVLITEFTDADALAALARRILDEIARPVTVNGQLLHMS
                     ASLGISVHPQDGADAEHLLQAAEAARAQIRHGLGNRLHFFTAGMDTRARRWIEVESEL
                     RHALARGELQLYYQPIVCVRDGRVRTVEALLRWNSPALGWVSPAEFIPVAEESELIVA
                     IGDWALETACRQVRHWQDTGLPAVRVAVNVSAHQLAGGRLPERVREVLERTGIDPGQL
                     ELELTESAMMSDSEHGARQVEAISRMGVSVALDDFGTGYSSLAYLSRFALTKLKIDRS
                     FVDGLSRDPKSSIIVDATLGLARGLGLRVVAEGVETEEQRALLAAAGCDALQGYLFSP
                     PVAPAALGALIDRFAPAERYAKPRVVAIGA"
     gene            complement(4556..5236)
                     /locus_tag="PKN25_07610"
     CDS             complement(4556..5236)
                     /locus_tag="PKN25_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV90801.1"
                     /translation="MEGMRVLLVDDHSLVRAGIRALLGSIAGVAGIEEASSGRQAVEL
                     AATMQPDLVLMDIAMDDMDGLAATRTIAAAHPHIKVVILSMHDTGDFFEQALRCGARG
                     YLLKDAAVAELEMAIAAVMRGDVYLSPAVSRHLVGNMLARPPEDERAASVLTPRQTEI
                     LELIGSGLGTKEIARRLDLSVKTVEAHRAQIMERLGVRDIANLLLEAARRGLITLGKR
                     STTLDAPR"
     gene            complement(5246..6799)
                     /locus_tag="PKN25_07615"
     CDS             complement(5246..6799)
                     /locus_tag="PKN25_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNV90802.1"
                     /translation="MTPPPNSAPAPSTPSPSPADSRSAPAPRELAVLMVEDSESDALL
                     VLRALRRAGFALRHRRVEDGAGMRAALAGERWDVVLSDFNLPGFDARSALAELHASGL
                     DLPFIVVSGTIGDEIAIELMRRGAHDYLMKENLARLAPAIERELGEARVRAERRRALA
                     ALHDSEAVLREAQRIGGVGQWRRDLPGGAPWCSDTLFALFGMAPVAGEDALDALLTRV
                     HPDDLGRLLQAHRELAAGSERVELEYRIVHADGSLRYVLECAARSRGVEAATPRLIGT
                     VHDVTAQRAAAEALRTANARLRQMSLRVLDAQEAERRAVAYELHDQIGQALTAVKLDL
                     QAMTPHLDAGPAVARLAAAIHTTEEALAQVRGLSLNLRPPQLDYMGLEASLRWHAERE
                     CARAGLALDFAGSVGEFKAEERCAIVCFRLLQEALTNVVRHAAAKRVRIALSAADGRL
                     DLEIADDGRGFDLARARRRMLEGASVGLLGMEERAALIGGEVHIDAAPGAGTRIRVSL
                     PCTAHAAAA"
     gene            complement(6810..8315)
                     /locus_tag="PKN25_07620"
     CDS             complement(6810..8315)
                     /locus_tag="PKN25_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV90803.1"
                     /translation="MLIRRQQWAIAIASLITVVVATGLWIANNLALRAELERGRMSAQ
                     IGPVGAAQMRSRIFEFLAEPSPAARARWYESHQALGALLAPALMDGTPGEPIIAGLRA
                     RHAELPALFEALAADRLREAAGSTPTPELLDAQRMASVHFMEATQQVASDTLRLLDLG
                     QARLERLHADQERLIAGLILALGLVIGLELFLVHRRILDPLQELHKGARAIEKGHYEH
                     RLGMSGKDEIAQVGAQFDHMADALGETLQALHQQRSQLELANRELESFSYSVSHDLRA
                     PLRGIGGWAQALEEDHGAALPVEAQEYLARIRGECARMNLLIEDLLQLARVTRSEPSF
                     EWLELDLLAREVAERVRESWPGRDIEFVIAPGLTVWGDRRLLGIALANLFDNACKFTA
                     RVPRARVELGCREIVDPLGARVLRQYFVRDNGAGFDMKHAARLFVPFQRMHSQREFPG
                     TGIGLAIVQRIIQRHAGVLEAEAGPGEGACFRFTLPLLPGPPAPVPNATPP"
     gene            8598..9803
                     /locus_tag="PKN25_07625"
     CDS             8598..9803
                     /locus_tag="PKN25_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV90804.1"
                     /translation="MSQAPQAPASKDSQLLPLAALAAPGLAGALLLGALVPGAAGVVA
                     AGVVGVAGLALPWWILRRERARQADVAARVATAERARLAEREAYLASLQQLAQAALAR
                     WHDHVGISRSQTETAVGALAGEFAHILERLQDALLRSRQSTADRGEDSVVGVIGHARG
                     ELDRVLAGLNAALAEKQSLSQAVARLAQVTEELMRMASEVGEIAKQTNLLALNAAIEA
                     ARAGEVGRGFAVVADEVRKLSDLSGATGERIRAKVESAHAAMAEALAAAEHMARGDAV
                     LVRDSEAAIGSVVGRFDQVGNAMAQAARALEEDSTAVQSRIEGVLVQLQFQDRVSQIL
                     EAVRADMARLAAQVAEDEARLAGGRVPAPIDVAAWIAALERTYTTLEQHARRADANPA
                     PAGGDITFF"
     gene            9820..10185
                     /locus_tag="PKN25_07630"
     CDS             9820..10185
                     /locus_tag="PKN25_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV90805.1"
                     /translation="MSKTVLIVDDSASLRQVVSLSLKGAGYQVIEACDGRDALGKLGG
                     QKVHLIISDVNMPNMDGIAFVREVKKNPSYKFTPVIMLTTEAAEEKKRLGQEAGARAW
                     VVKPFQPQQMLAAVSKLVM"
     gene            10197..10532
                     /locus_tag="PKN25_07635"
     CDS             10197..10532
                     /locus_tag="PKN25_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV90806.1"
                     /translation="MDTPALDTPAAGVARLAPEGELGIYTVAALREAWLQALATHEAL
                     EIDLGRIDEIDTAGLQLMLMAKRSVGRSVRFVNHSAAVLRLLDLARLGGALGDPLLED
                     AAPATQEAR"
     gene            10532..12733
                     /locus_tag="PKN25_07640"
     CDS             10532..12733
                     /locus_tag="PKN25_07640"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNV90807.1"
                     /translation="MDLSQALATFLDESRELLAQMEEILLRAETAALPTDELHALFRC
                     AHTIKGSAGLFGLDAVVHFTHAVESVLDRMRNGELELSDALINLLLESQDHIASLVAA
                     VAAELPPPEFEGAPLRARLEAFCAATPATPAALPATVTEGDPVVCSATEAAPALEVGG
                     SVALGAEHWHLSLRFAPEVLRNGMDPLAFIHYLRTLGELVHVETIADTLPELAAGDPE
                     NCYLGFEIALRSTAPRAEIEAVFEFVQDSARIVLVPPAARVEEYLALIEALDEDPRRL
                     GEILVTCGSVTARELEAALAAQHVQAEAPRVGELLVEHAGVPAQVVQAALGKQKAAEE
                     RRASETRSVKVPADRLDALIDQVGELVIAGAATQLQAQQAGSRALQETAANLLRLVED
                     VRDAALRLRMTPIGEVFNRFPRVVRDLARELGKDIELVIRGAEAELDKSMVEKIGDPL
                     MHLVRNALDHGIESPERRAAAGKPARGRVELRAFHESGSIVIEVADDGGGLDARRILA
                     KAVERGLVAADAVLSEAEIHRLVLAPGFSTAAELTSLSGRGVGMDVVKSSVDALRGTL
                     DIHSAPGCGTTMRLCLPLTLAIIDGFQVGVAGATFILPLDAVVECIELPADAGAAGYL
                     DLRGQVLPFLRLRALFALEGAPPPRQNVVVVRFGGRQAGIAVDHLLGECQAVIKPLGP
                     LFERVAGIAGSTILGSGEVALILDVPQLVQRAIAHEGRDTGGAPRARFLAA"
     gene            12781..14436
                     /locus_tag="PKN25_07645"
     CDS             12781..14436
                     /locus_tag="PKN25_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV90808.1"
                     /translation="MKVGMKLGLGFGLVLVLLGTLLAVGILRLTDIKHDVDVVVDELW
                     PKTQLLQDGLVGVNEIALGGRELVIAENGEQIGRAKDRMLGGRERIGKAWDALAPMLV
                     QPRGKELFAEVLDSRQRFIPAQNRLIELVEQGRADEARAFLRGDYDAIAVQYRQRVNA
                     LTAYQGELLSGTGSATAATVAAATLTMSVLGGIALLVGVAVAILITRGLLRQLGGEPD
                     YAAEIARKVAAGDLTVDVQLRAGDSASVLAAMQRMVGSLSHTLGEVRGAADALSAASE
                     QVSATSQSLAQGSSEQAASLEETSASIEQMSASIGQNTENARVTDGMASKSAADADEG
                     GDAVRATVEAMRSIADKIGIVDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVRK
                     LAERSQVAAQEIGELAGSSVKTAERAGALLEQIVPSIRKTAELVQEITASSEEQSAGA
                     GQINIAMSQISQTTQQNASASEELSATAEEMNSQAEQLQRLVAQFRLDPQAQLAGGDG
                     GAARTTRGQPAPARSTREPRRIGLGNAQPAFASAAADPEYVRF"
     gene            14449..14988
                     /locus_tag="PKN25_07650"
     CDS             14449..14988
                     /locus_tag="PKN25_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV90809.1"
                     /translation="MSQSSLQPVVPATASLRAAAAAGGQYLVFTLGGEVFAIDILQIR
                     EIIEFGELTEVPMTPPTVRGVINLRGAVVPVIDLAARFGRERIRSGRRSCIVIVEVGG
                     EAGTQTLGVMVDGVSEVIDIAAADIEPAPSFGARIRTDFIAGMARREGRFVILLEVGR
                     VFSIDELAGIGALAGAAEH"
     gene            15012..15821
                     /locus_tag="PKN25_07655"
     CDS             15012..15821
                     /locus_tag="PKN25_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNV90810.1"
                     /translation="MSEISITQAEFEQFRALMHRAAGVDLPPQKISLVSGRLARRLRA
                     LGLASFGDYHRLVSSAGAHDEFERMVDLLTTHETYFFREPKHFDFLAESVLPALGRTR
                     TPRFWSAASSSGEEAYSLAMVLADRLGGDAPWEVFGSDISREVVEKARGGVYAMNRID
                     GIPPDYLRRYCLRGVGSRDGTLQVDAALRRRVRFAQVNLNGNLAGVGDFDVVFLRNVL
                     IYFDLPTKRRIVERIVRQMRPGGWLFIGHSESLNGLDLGLRQERPTIYRRM"
     gene            15824..16408
                     /locus_tag="PKN25_07660"
     CDS             15824..16408
                     /locus_tag="PKN25_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNV90811.1"
                     /translation="MRPASRNTIEIFLQPGEFWFGDRDTRIRTLLGSCVSITMWHPRL
                     RIGGMCHFMLPERGVRACADGLDGRYAGDAIALFLRELRAAHTRPQDYQVKLFGGGRM
                     FRSRHAAGAPAALDVGTRNVEAARMLLRTHGFRVRAAHVEGEGHRNLIFDIGSGEVWL
                     RHVAPAAASAAVIHTEVPSQAHPGVHPESASEAA"
     gene            16405..17679
                     /locus_tag="PKN25_07665"
     CDS             16405..17679
                     /locus_tag="PKN25_07665"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNV90812.1"
                     /translation="MNAPIRVLVVDDSAVVRQVVSEILSAAPGIEVVGTASDPLFAMA
                     KMRRDWPDVITLDVEMPRMDGISFLRQLMAERPTPVIVCSTLTSRGAETTMQALAAGA
                     VSIIAKPTLGLKGFLTDSAQELVAAVRAAARANLRALRAGAGVGGGGPGVGGRAGSGA
                     EIGSATGPGGAGVAAAPGVGVAAARGAAGAGPAGSGARGGSNGAPGGALARPAAATAI
                     VPAAVAPARAEAMAETTDRIIALGTSTGGTQALEAVLRGLPRTVPGIVVVQHMPEKFT
                     AMFAQRLDQVCALEVLEARDGQRVLPGRVLIAPGGRHMTVRRSGAQYLVEVRDGPPVN
                     RHRPSVDVLFRSVARCAGRNALGVIMTGMGDDGARGLREMFDAGAQTIAQDEASSVVF
                     GMPKEAIRLGAATRILALQAIGPAIEQYTRAS"
     gene            18245..19177
                     /locus_tag="PKN25_07670"
     CDS             18245..19177
                     /locus_tag="PKN25_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNV90813.1"
                     /translation="MQDLSALCRPMSKVFISYRRDDSAGFAGRLADALERRFGADSVF
                     RDVDDIAAGADFGVVIEQALQQVQAVLVVIGTRWLDARDGRGRRLDDPGDYVRREVEL
                     ALASGKPLIPVLVGGATMPAAEALPATLRGLANRNALALGDAGWVADLARLEAALVQW
                     VAPPVSPAPVRGRRALAAAIAALVLALGGGWWWLRDGDGGLQAIVGAWSAEVAYPWNL
                     TLREHYVFTLEDGRIGGSASFLGVPRALEAVAWGDGRLRFLTRSETVSGNEPPREMGQ
                     LYEVWREGETLRVRLQIRRSHSVDAALEFTAQRQ"
BASE COUNT         2582 a         6966 c         7017 g         2695 t
ORIGIN      
        1 accgcggcga aggtcacgcc catcatcacc ggcagcttga tgccgaagta cttgccgaag
       61 ccgagcgact ggatcagcgt caccaggccg cagcagaaca ggtcggcgga gatgagcagg
      121 gcgacctgct cggggccgaa gccgagcgcg cgccccacga tcaggggcac cgcgaccgcg
      181 ccggcataca tcaccagcac gtgctgcatg cccaacgcgg cgagccgccc gccgggcagt
      241 ttctcgtcga ccggatggac cgggtcgctt ccgccttgca gtgcgaggtc ggacatgttc
      301 gtctccttca tgttttgtgt gtctcttccg cgccgtcccg cccgggtggt cccgggtcat
      361 cgcggcgccg tgcaggggtc ggggtggtgg tgcgccctct gccgtcgggg aggggcggag
      421 ggcgcggcca gggtctgctg ccgacttgaa ccggagtcta gggattcgcc ttcgcggcgg
      481 gagcgagtct ggcgaatacc tcgaatataa aattttatat ttttgcggtg cagcatcacg
      541 tggcgtgaag ggctcgcagc ggcatccgcg ggcggcgcgc attcagtaca gtgcgccggt
      601 cagcagcagg tagccgggca gccaggcggt gcccatgccc tggacgatcg ccatccacgc
      661 ggtcgcgcgc gcgatcggtc tgtgcagggc cagcagcagg aagaacatca gccacagcac
      721 cgcccacgcc gcccacgaca ggccgagcca ccagtcccag gtgctgtcgg cggcgcgcag
      781 ggtgtcggcc gcgatcggca gcgcggtgat cgccacgaac aggctgaacc agcccagccc
      841 gcgcccgtcg gcgccggtga ggcggttcca ggccacccac aggtaggtga aggagaacag
      901 cagcgagaag gccgcggcgc ggatcgacgc cgcgtcggcg tcggcgccga gggccaggcg
      961 caggcagacc agcagggtga gtccgccggt gcagaggttg atgatggtga tctcgcggtc
     1021 gccgatgcgg ccgagcatcc acaggccgtt gaggatcagg accgcgccga cgtagaacag
     1081 ggacaggccg agcagcatga ggttttccag ggacgtacga tggggccgcg cagtgtaggt
     1141 ggccgagacg cgcgcgcgca gtgcggcagg ctgatgccgc gcatcagggc gcgcacacgt
     1201 tgcgcttccc cgtcgcgcga cgccgcgtgc cgatcgcccg ctccccgcgg atgggctaca
     1261 tcaccgatgc gatatgacac ccccggcggc gctggcgcat aatccgtccc gcactgttgc
     1321 tcgccgccgt ctccccggcg gatcccttcg gccgcggctc ccttccgggg cgccgcggca
     1381 ttttcctttt ggaggtccga tgtccgcatc cggctcgccg cgcctggcgc tgtccgcgct
     1441 cgcgctcctg tcgctgatct ggggctacaa ctgggtggtc atgaagcagg tcatccagta
     1501 cgtcgatccc ttcgacttct cggcgatccg caccgcgctc ggcgccgcca cgctcttcgt
     1561 ggtgctgatc gcactgcgtc gcccgctggg gctgcagcag gtgcgcgagg tggcgctgct
     1621 cggcgtgctg cagaccggcg ccttcacggc gctgatccag tgggcgctgg tggccggcgg
     1681 cgcgggcaag accgcggtgc tggtgtatac gatgccgttc tgggtggtgc cgatggcctg
     1741 gttcgtcctc ggcgagcgcg tgcgcggcat gcagtgggtg gccaatgcga tcgccggctg
     1801 cggcctcgtg ctggtgctcg agccctgggc gatgggcggc agcgccttca gcaacctgct
     1861 cgcggtcgcc ggcggggtga cctgggcggc ggcctcggtg caggccaagc gcatgcgccg
     1921 cgcgcacgac ttcgacctgc tctcgctcac cgcctggcag atgctgttcg gcgcggtcgc
     1981 gctgtgcgtg ctggcgctgc tccacccctc gcggccgatc gacccgacgc cttacttctg
     2041 gggcgcgctg gtgttcaacg cggtgttcgc caccgggctg gcctggctgc tgtggctgtt
     2101 cgtgctgcag aggctgtccg ccggcgtggc ggggctgtcc gcgctcggca tcccgatggt
     2161 gggtgcgctc gcgggctgga tcgagctcgg cgagcgcccc accgcggtgg aattcgccgg
     2221 catggcgctg atcgtcggcg cgctggcgct gacgagcctg tggtcgctgc gccaggccag
     2281 gcgcgcgtcg ccgcgctgag cggcgggcgc gccgcgctca ggcgccgatc gcgaccacgc
     2341 gcggctttgc gtagcgctcg gcgggcgcga agcggtcgat cagcgcgccg agggccgcgg
     2401 gcgccaccgg cgggctgaac agatagcctt gcagcgcgtc gcagccggcg gcggcgagca
     2461 gcgcgcgctg ctcctccgtt tccacgcctt cggcgaccac gcgcaggccc aggccgcgcg
     2521 ccaggcccag ggttgcatcc acgatgatcg agctcttcgg gtcgcggctc aggccatcga
     2581 cgaagctgcg gtcgatcttc agcttggtga gggcgaagcg gctcaggtag gcgagcgagg
     2641 agtagccggt gccgaagtcg tcgagcgcga cgctcacccc catgcggctg atcgcctcga
     2701 cctggcgcgc gccgtgctcg ctgtcgctca tcatcgcgct ctcggtgagc tcgagctcga
     2761 gctggccggg gtcgatgccg gtgcgctcga gcacctcgcg cacgcgctcg ggcaggcgcc
     2821 cgcccgcgag ctggtgcgcg gagacgttca ccgccacgcg caccgccggc aggccggtgt
     2881 cctgccaatg gcggacctgg cggcaggcgg tctcgagcgc ccagtcgccg atcgcgacga
     2941 tcagctcgct ctcctcggcc accgggatga actccgccgg cgacacccag cccagcgcgg
     3001 ggctgttcca gcgcagcagc gcctccacgg tgcgtacgcg gccatcgcgc acgcacacga
     3061 tcggctggta gtagagctgc agctcgccgc gcgcgagcgc atggcgcagc tcgctctcga
     3121 cctcgatcca gcgccgtgcc cgggtgtcca tgccggcggt gaagaagtgc aggcggttgc
     3181 ccaggccgtg gcggatctgc gcacgcgcgg cctcggccgc ctgcagcagg tgctcggcgt
     3241 cggcgccgtc ctgaggatgg acgctgatgc ccaggctcgc gctcatgtgc aggagctggc
     3301 cgttgaccgt caccgggcgg gcgatctcgt cgaggatgcg tcgcgcgagc gcggccagcg
     3361 cgtcggcatc ggtgaactcg gtgatcagca cgaggaactc gtcactgccc aggcgggcga
     3421 ggctgctgcg ccgcttcagg cagccggcga tgcgctcggc catcacttgc agcaggcggt
     3481 cgccggcgtc ctgaccgagg ctctcgttca cccgccggaa gcggtcgagg tcgagcagca
     3541 gcaccgcggc cttgccctcg ccggcggcgg cgcgctccac cgcctgctgc agccgctcca
     3601 tgaagagctg gcggttggcg aagccggtga ggggatcgtg gtgggtcagg tagtcgagcc
     3661 gggcctggtc ctgcttctcg cggctgatgt cgctgaacat gcccacgtag tgcgtgggca
     3721 ggccgtcggc gccgcgcacc gcacgcaatg ccaggcgctc ggggtagatc tcgccgctct
     3781 tgcgccggtt ccacacctcg ccgacccacg agccgcgcgt gaccagcgcg tcccacatgc
     3841 cggcgtagaa ggcggcgtcg tggcggccgg atttgagcac gcgcggattg agcccgagca
     3901 cctcggcgcg cgcatggccg gtgatgcgct cgaaggccgg gttgcagtcg aggatgtcgc
     3961 cggcgaggtt ggtcaccacg atgccttcgt gcaggccctc gacgacctcg ccccagatgc
     4021 gcaggtgggc ctcgcgggca cgcaggccgt cctcggcgtg gcggcgggcc cggcgcccgc
     4081 gcgcctcggc caggccgcgc gccagcgccg gggcgaggcg cgcgagatcg tgcttgtgca
     4141 cgtagtcgtg tgcgccgagc gccatcaggc ggccggcctc ctcgtcggcg agcgaggccg
     4201 agacgaccac gaagggcagg tcgaggccgc tcgcctgcac ctgggcgagg gcctcctcgg
     4261 ggctgaaccc gggcagggcg tagtcgcaca gcaccgcgga ccacgcgcag gcctcgagcg
     4321 cggcgccgag ttccgcggcg cgatcgacgc ggcgcgccgt cgccaccgtg tagccggcgc
     4381 gctcgagctg gcgcacgacc agagcggcat cggattcgtt gtcctcgacg atcagcaggt
     4441 tgacggtctc gcccatgctt gcccttccat tgcggggtgc ggccgcaagc gcactccggc
     4501 actccggtcc atgcggcgat gataagccgg cgggccgtgg cggcaatccc ttggattacc
     4561 gaggcgcgtc tagggttgtg gagcgcttgc cgagcgtgat gaggccgcgg cgcgcggcct
     4621 cgagcagcag gttggcgatg tcgcgcacgc ccaggcgctc catgatctgc gcgcggtgcg
     4681 cctccacggt cttgacgctg aggtcgagcc ggcgggcgat ctccttggtg ccgaggccgc
     4741 tgccgatgag ctcgaggatc tcggtctggc gcggggtcag cacgctcgcc gcgcgttcgt
     4801 cctcgggcgg acgggcgagc atgttgccca ccaggtggcg cgacaccgcc gggctcagat
     4861 agacgtcgcc gcgcatcacc gccgcgatcg ccatctcgag ctcggccacc gcggcgtcct
     4921 tgagcaggta gccgcgcgcg ccgcagcgca gcgcctgctc gaagaagtcg ccggtgtcgt
     4981 gcatcgacag gatcaccacc ttgatgtgcg gatgcgccgc ggcgatcgtg cgtgtggcgg
     5041 cgaggccgtc catgtcgtcc atcgcgatgt ccatcagcac caggtcgggc tgcatcgtcg
     5101 ccgccagctc gaccgcctga cggccgctgc tcgcctcctc gatgccggcc acgcccgcga
     5161 tggagccgag cagcgcgcgg atgcccgcgc gcaccaggct gtgatcgtcc accagcagaa
     5221 ctcgcattcc ttccatggcc gaatcctacg ccgcggcggc gtgtgcggta cagggcaggc
     5281 tgacccggat gcgggtgccc gcgccgggcg ccgcgtcgat gtggacctcg ccgccgatga
     5341 gcgcggcgcg ctcctccatg ccgagcaggc ccacgctggc gccctccagc atgcgccggc
     5401 gcgcgcgcgc gaggtcgaag ccgcggccgt cgtcggcgat ctccaggtcc aggcggccgt
     5461 ccgcggccga cagggcgatg cgcacccgct tcgccgccgc atggcggacg acgttggtga
     5521 gcgcctcctg cagcaggcgg aagcacacga tcgcgcagcg ctcttccgcc ttgaactcgc
     5581 ccacgctgcc ggcgaagtcg agcgccagcc cggcgcgcgc gcattcgcgc tcggcgtgcc
     5641 agcgcagcga ggcttccagg cccatgtagt cgagctgcgg cgggcgcagg ttcagggaca
     5701 ggccgcgcac ctgcgcgagc gcctcctcgg tggtgtggat ggccgcggcc aggcgcgcca
     5761 ccgccgggcc ggcgtccaga tgcggggtca tcgcctgcag gtcgagcttg accgcggtca
     5821 gcgcctggcc gatctggtcg tgcagctcgt aggccaccgc acggcgctcg gcctcctggg
     5881 cgtcgagcac gcgcagcgac atctggcgca ggcgcgcgtt ggcggtgcgc aaggcctcgg
     5941 ccgcggcgcg ctgcgcggtg acatcgtgca ccgtgccgat caggcgcggc gtcgcagcct
     6001 cgacgccgcg gctgcgggcg gcgcactcga gcacgtagcg caggctgccg tcggcatgga
     6061 cgatgcggta ctccagttcc acgcgctcgc tgccggccgc gagctcgcga tgcgcctgca
     6121 gcaggcggcc gaggtcgtcc gggtgcacgc gggtgagcag ggcgtcgagc gcatcctcgc
     6181 ccgccaccgg ggccatgccg aagagcgcga acaaggtgtc cgagcaccat ggcgcgccgc
     6241 ccggcaggtc cctgcgccac tgcccgaccc cgccgatgcg ctgcgcctcg cgcagcaccg
     6301 cctcgctgtc gtgcagggcg gcgagcgcgc gtcggcgctc ggcgcggacg cgcgcctcgc
     6361 cgagctcgcg ctcgatcgcg ggggcgaggc gggcgaggtt ctctttcatc aggtagtcgt
     6421 gggcgccgcg gcgcatcagc tcgatcgcaa tctcgtcgcc gatggtgccc gacaccacga
     6481 tgaagggcag gtcgagcccg ctcgcgtgca gctcggcaag cgcactgcgc gcgtcgaagc
     6541 cgggcaggtt gaagtcggag agcaccacgt cccagcgctc gcctgcgagc gccgcgcgca
     6601 ttccggcacc gtcctcgacg cggcgatggc gcagcgcgaa gccggcacgc cgcagcgcac
     6661 gcaggaccag cagcgcatcg ctctcggagt cttccaccat cagcaccgcg agctctcgtg
     6721 gcgcgggtgc gctgcggctg tccgcaggcg agggcgaggg cgtggagggg gcaggggcgg
     6781 agttcggggg aggggtcaag gcgctcgcct catgggggcg ttgcgttcgg tacgggggca
     6841 ggcggtccgg gcagcagcgg cagggtgaag cggaagcacg cgccctcgcc gggcccggcc
     6901 tcggcctcca gcacgccggc gtgacgctgg atgatacgct gcacgatcgc cagcccgatg
     6961 ccggtgccgg gaaactcgcg ctgcgagtgc atgcgctgga agggcacgaa cagccgggcg
     7021 gcgtgcttca tgtcgaagcc cgcgccgttg tcgcgcacga aatactgccg cagcacgcgc
     7081 gcgccgaggg gatcgacgat ttcccggcag ccgagttcga cgcgcgcccg cggcacgcgc
     7141 gcggtgaact tgcaggcgtt gtcgaacagg ttggcgagcg cgatcccgag caggcggcgg
     7201 tcgccccaga ccgtcagccc gggggcgatc acgaactcga tgtcgcgccc cggccagctc
     7261 tcgcgcacgc gctcggccac ctcgcgggcg agcaggtcga gctccagcca ctcgaaggac
     7321 ggctcgctgc gggtgacgcg ggccagctgc agcaggtcct cgatgagcag gttcatgcgc
     7381 gcgcattcgc cgcggatgcg tgcgaggtat tcctgcgctt ccacgggcag ggcggcgccg
     7441 tgatcctcct cgagcgcctg cgcccagccg ccgatgccgc gcagcggtgc gcgcaggtcg
     7501 tgcgacaccg agtagctgaa gctctccagc tcgcggttgg cgagttcgag ctggctgcgc
     7561 tgctggtgca gggcctgcag ggtctcgccg agcgcgtcgg ccatgtggtc gaactgcgca
     7621 ccgacctggg cgatctcgtc cttgccggac atgccgaggc ggtgctcgta gtgacccttc
     7681 tcgatcgccc gcgcgccctt gtgcagttcc tgcagcggat cgaggatgcg gcgatgcacc
     7741 aggaagagct ccaggccgat caccaggccc agtgcgagga tgaggcccgc gatcaggcgc
     7801 tcctgatcgg catgcagccg ttccagccgg gcctggccga gatcgagcag gcgcaaggtg
     7861 tcgctggcca cctgctgcgt cgcctccatg aagtgcaccg aggccatgcg ctgcgcgtcg
     7921 agcaactcgg gcgtcggcgt gctgcccgcg gcctcgcgca ggcgatcggc ggcgagcgcc
     7981 tcgaacagcg ccggcagttc cgcgtggcgt gcgcgcaggc ccgcgatgat cggctcgccg
     8041 ggggtgccgt ccatcagcgc gggcgcgagc agcgcgccga gcgcctggtg gctctcgtac
     8101 cagcgcgcgc gcgcggccgg cgagggctcg gcgaggaact cgaagatgcg cgagcgcatc
     8161 tgcgcggcgc ccaccggccc gatctgcgcc gacatgcggc cgcgctccag ttcggcgcgc
     8221 aaggccaggt tgttcgcgat ccacagcccc gtggcgacca cgacggtgat cagcgacgcg
     8281 atcgcgatcg cccattgctg cctgcggatc agcacgcgaa tccctccgtc tcgatgagcc
     8341 tgctcctgct ttccgactag tccaatcccc taggggcatg cacggaattt taccgattgt
     8401 ccctagacgc cccggagcgg gaacatgcag gcgacggaaa tgcatgccgc cggcaaaccc
     8461 cgggcggatg cggagtttaa cggcagtccc cggatgggtt ccatcttcgc gcggagcgct
     8521 ccgtgagcga agtccgcacg gaacaggaat cgccgcagcg ccacaaggcg ggtgcggcac
     8581 aagtggagaa gcctcaagtg tcccaagccc cccaggctcc cgcctcgaag gattcgcagc
     8641 tcctgcccct ggctgcgctc gccgcgcccg ggttggcggg ggcgctgctg ctcggcgcgc
     8701 tcgtgccggg cgcggcgggc gtcgtcgcgg cgggcgtcgt cggcgtcgcc ggcctcgccc
     8761 tgccatggtg gatactgcgt cgcgagcgcg cccgccaggc tgacgtcgcg gcgcgcgtgg
     8821 ccacggcgga gcgtgcgcgc ctggccgagc gcgaggccta cctcgccagc ctgcagcagt
     8881 tggcgcaggc cgcgctggcg cgctggcacg accacgtggg catctcgcgc agccagaccg
     8941 agaccgccgt gggcgcgctc gccggcgagt tcgcgcacat cctcgagcgc ctgcaggacg
     9001 cgctgctgcg ctcgcgccag tccaccgccg accgcggcga ggacagcgtg gtcggggtga
     9061 tcggccatgc ccgcggcgag ctcgaccgcg tgctcgccgg gctcaacgcc gcgctcgccg
     9121 agaagcagag cctgtcgcag gcggtcgccc gcctcgccca ggtcaccgag gagctgatgc
     9181 gcatggcgag cgaggtcggc gagatcgcca agcagaccaa cctgctcgcg ctcaacgcgg
     9241 cgatcgaggc cgcccgcgcc ggtgaggtgg ggcgcggctt cgcggtggtc gccgacgagg
     9301 tgcgcaagct ctccgacctc tccggcgcca ccggcgagcg tatccgcgcc aaggtcgaga
     9361 gcgcccacgc cgcgatggcc gaggcgctcg cagcggccga gcacatggcc cgcggcgacg
     9421 ccgtcctggt gcgcgactcc gaggcggcga tcggcagcgt ggtcggccgc ttcgaccagg
     9481 tcggcaacgc catggcgcag gccgcgcgcg cgctcgagga agacagcacc gcggtgcagt
     9541 cccgcatcga gggcgtgctg gtgcagctcc agttccagga ccgcgtcagc cagatcctcg
     9601 aggcggtgcg cgccgacatg gcgcgcctcg ccgcccaggt cgccgaagac gaggcccgcc
     9661 tcgccggcgg gcgggtgccg gcgccgatcg acgtcgccgc ctggatcgcc gcgctcgagc
     9721 gcacctacac caccctcgaa caacacgccc gccgcgccga cgcgaacccc gccccggctg
     9781 gcggcgacat caccttcttt tgagcaggag tactccccca tgtccaagac cgtgctgatc
     9841 gtcgacgact ccgcctcgct gcgccaggtg gtgagcctgt cgctcaaggg cgccggctac
     9901 caggtgatcg aggcctgcga cggccgcgac gccctcggca agctcggcgg ccagaaggtt
     9961 catctcatca tctccgacgt gaacatgccc aacatggacg gcatcgcctt cgtgcgcgag
    10021 gtcaagaaga acccgagcta caagttcacc ccggtgatca tgctcaccac cgaggccgcc
    10081 gaggagaaga agcgcctcgg ccaggaagcc ggcgcgcgcg cctgggtggt caagcccttc
    10141 cagccgcagc agatgctggc ggcggtgtcc aagctggtga tgtgagcgcg ccgcccatgg
    10201 acacccctgc actcgacacc cccgcggccg gcgtcgcccg cctcgccccc gagggcgagc
    10261 tcggcatcta caccgtggcc gcgctgcgcg aggcctggct gcaggcgctc gccacccacg
    10321 aagcgctcga gatcgacctc ggccgcatcg acgagatcga caccgccggc ctgcagctca
    10381 tgctgatggc caagcgcagc gtcgggcgca gcgtgcgctt cgtcaaccac tccgccgcgg
    10441 tgctgcgcct gctcgacctc gcccgcctcg gcggcgcgct cggcgacccg ctgctcgagg
    10501 acgccgcgcc ggccacgcag gaggcacgct gatggacctc tcccaggccc tggccacctt
    10561 cctcgacgag agccgcgagc tgctcgcgca gatggaggag atcctcctgc gcgccgagac
    10621 cgccgcgctg ccgaccgacg agctgcacgc gctgttccgt tgcgcccaca ccatcaaggg
    10681 ctcggccggc ctgttcgggc tggacgcggt ggtgcacttc acccacgccg tcgagagcgt
    10741 gctcgaccgc atgcgcaacg gcgaactcga actctccgac gccctgatca acctgctgct
    10801 cgagtcgcag gaccacatcg ccagcctcgt cgccgcggtc gccgccgaac ttcctccgcc
    10861 cgagttcgag ggcgcgccgc tgcgcgcccg cctcgaagcc ttctgcgccg cgacacccgc
    10921 cacccccgcc gcactgccgg cgaccgtgac ggagggggac ccggtcgtct gctccgcgac
    10981 cgaggccgcg cccgcgctcg aggtcggcgg cagcgtggcg ctcggcgccg agcactggca
    11041 cctgtcgctg cgcttcgcgc ccgaggtgct caggaacggc atggacccgc tcgccttcat
    11101 ccactacctg cgcaccctcg gcgagctcgt gcacgtcgag accatcgccg acaccctgcc
    11161 cgagctcgcc gccggcgacc cggaaaactg ctacctcggc ttcgagatcg cgctgcgcag
    11221 cacggccccc cgcgccgaga tcgaggcggt gttcgagttc gtgcaggaca gcgcgcgcat
    11281 cgtgctggtg ccgccggcgg cgcgcgtcga ggagtacctc gccctcatcg aggcgctcga
    11341 cgaggacccg cgccgactcg gcgagatcct cgtgacctgc ggctcggtca ccgcgcgcga
    11401 gctcgaggcc gcgctcgccg cccagcacgt gcaggccgag gcgccgcgcg tgggcgagct
    11461 actggtcgag cacgccggtg tgcccgccca ggtggtgcag gccgcgctcg gcaagcagaa
    11521 ggccgccgag gagcgccgcg ccagcgagac gcgcagcgtc aaggtgcccg ccgaccggct
    11581 cgacgcgctc atcgaccagg tcggcgagct cgtcatcgcc ggcgccgcca cccagctgca
    11641 ggcgcagcag gccggcagcc gcgcgctgca ggagaccgcc gccaacctgc tgcgcctggt
    11701 cgaggacgtg cgcgacgccg cgctgcgcct gcgcatgacc ccgatcggcg aggtcttcaa
    11761 ccgcttcccg cgcgtggtgc gcgacctcgc gcgcgagctc ggcaaggaca tcgagctggt
    11821 gatccgcggc gccgaggccg agctcgacaa gtccatggtc gagaagatcg gcgacccgct
    11881 catgcacctc gtgcgcaacg ccctcgacca cggcatcgaa tcgcccgaac gccgcgccgc
    11941 ggcgggcaag ccggcgcgcg gccgggtcga gctgcgcgcc ttccacgagt cgggcagcat
    12001 cgtcatcgag gtcgccgacg acggcggcgg gctggacgcg cggcgcatcc tcgccaaggc
    12061 cgtcgagcgc ggcctggtcg cggcggacgc cgtgctctcc gaggccgaga tccaccgcct
    12121 cgtcctggcg ccgggcttct ccaccgcggc cgaactcacc agcctgtccg gccgcggcgt
    12181 cggcatggac gtggtcaaga gcagcgtcga tgcgctgcgc ggcaccctcg acatccacag
    12241 cgcgccgggc tgcggcacca cgatgcggct gtgcctgccg ctcacgctgg cgatcatcga
    12301 cggcttccag gtcggtgtcg ccggcgccac cttcatcctg ccgctcgacg cggtggtcga
    12361 gtgcatcgag ctgccggccg acgccggcgc cgccggctac ctcgacctgc gcggccaggt
    12421 cctgcccttc ctgcgcctgc gcgccctgtt cgcgctcgag ggcgcaccgc cgccgcggca
    12481 gaacgtggtc gtggtgcgct tcggcgggcg ccaggcgggg atcgcggtgg accatctgct
    12541 cggcgaatgc caggcggtga tcaagccgct cgggccgctc ttcgagcgcg tcgccggcat
    12601 cgccggctcc accatcctgg gcagcggcga ggtggcgctg atcctggacg tgccacaact
    12661 ggtccagcgt gcgatcgcgc acgagggccg cgacaccggg ggtgcgccgc gggcgcgctt
    12721 ccttgctgca tgaatgctgc agaagaaacc ggaaacaggg gaaagcgaat gttcaggaac
    12781 atgaaggtgg gtatgaagct ggggctgggc ttcggcctcg tgctggtgct gctggggacg
    12841 ctgctcgcgg tgggcatcct gcgcctgacc gacatcaagc acgatgtcga cgtggtggtg
    12901 gacgagctgt ggcccaagac gcagctgctg caggacggac tggtcggggt caacgagatc
    12961 gccctcggcg ggcgcgagct ggtcatcgcc gagaacggcg agcagatcgg ccgtgccaag
    13021 gaccgcatgc tcggcgggcg cgagcgcatc ggcaaggcct gggacgcgct cgcgccgatg
    13081 ctggtgcagc cgcgcggcaa ggagctcttc gccgaggtgc tcgacagccg ccagcgcttc
    13141 atcccggcgc agaaccgcct catcgagctc gtcgagcagg gccgtgccga cgaggcgcgc
    13201 gccttcctgc gcggcgacta cgacgccatc gcggtgcagt accgccagcg cgtgaacgcg
    13261 ctcaccgcct accagggcga actgctctcc ggtaccggca gcgcgaccgc cgcgaccgtc
    13321 gccgcggcca cgctgaccat gtcggtgctg ggcggcatcg ccctgctggt gggcgtggcg
    13381 gtggcgatcc tgatcacccg cggcctgctg cgccagctcg gcggcgagcc cgactacgcc
    13441 gcggagatcg cgcgcaaggt cgccgccggc gacctcacgg tggacgtgca gctgcgtgcc
    13501 ggcgacagcg ccagcgtgct ggcggccatg caacgcatgg tgggcagcct gtcgcacacc
    13561 ctgggcgagg tgcgcggcgc ggccgacgcc ctgtcggcgg cctccgagca ggtgagcgcg
    13621 acctcgcagt cgctggcgca gggctcgtcc gagcaggccg ccagtctgga ggagacctcg
    13681 gcctcgatcg agcagatgtc ggcctcgatc ggccagaaca ccgagaacgc ccgcgtcacc
    13741 gacggcatgg cgagcaagtc cgccgccgac gccgacgagg gcggcgatgc ggtgcgcgcg
    13801 acggtggaag cgatgcgttc gatcgccgac aagatcggca tcgtcgacga catcgcctac
    13861 cagaccaacc tgctcgcgct caacgccgcg atcgaggcgg cgcgtgccgg cgagcacggc
    13921 aagggtttcg cggtggtggc ggccgaggtg cgcaagctcg ccgagcgcag ccaggtcgcg
    13981 gcgcaggaga tcggcgaact ggccggctcc agcgtgaaga ccgccgagcg cgcgggtgcg
    14041 ctgctcgagc agatcgtgcc ctcgatccgc aagaccgccg agctggtgca ggagatcacc
    14101 gcctcgtccg aggagcagtc ggccggcgcg gggcagatca acatcgccat gagccagatc
    14161 agccagacca cgcagcagaa cgcctcggct tcggaggagc tgtcggcgac cgccgaggag
    14221 atgaacagcc aggccgagca gctgcagcgc ctggtggcgc agttccgcct cgacccgcag
    14281 gcgcagctcg ccggcggcga tggcggcgcc gcgcgcacca cccgcggcca gccggcgcct
    14341 gcgcgcagca cgcgcgagcc gcgccgcatc ggcctcggca acgcgcagcc ggctttcgcg
    14401 tccgccgccg cggacccgga atacgtgcgc ttctgagcgg ggagcgccat gtcgcaatcc
    14461 agtcttcagc cggtcgtccc cgccactgcc agcctgcgtg ccgccgcggc cgcgggcggc
    14521 cagtacctgg tgttcacgct cggcggcgag gtcttcgcca tcgacatcct gcagatccgt
    14581 gagatcatcg aattcggcga gctcaccgag gtgccgatga cgccgcccac cgtgcgcgga
    14641 gtgatcaacc tgcgtggcgc ggtggtgccg gtgatcgatc ttgccgcgcg cttcggccgc
    14701 gagcgcattc gcagcgggcg gcgcagctgc atcgtcatcg tcgaggtggg tggcgaggcc
    14761 ggcacgcaga cgctgggcgt gatggtcgac ggcgtcagcg aggtcatcga catcgccgcc
    14821 gccgacatcg agccggcgcc gtccttcggg gcccgcatcc gcaccgactt catcgccggc
    14881 atggcgcgcc gcgaagggcg cttcgtgatc ctgctcgagg tcgggcgggt gttctcgatc
    14941 gacgaactcg ccggcatcgg cgcgctcgcc ggcgccgccg agcactgagc cggtcctccg
    15001 ggaggccacg catgtccgag atcagcatca cccaggccga gttcgagcag ttccgcgcgc
    15061 tcatgcatcg ggccgccggc gtcgacctgc cgccgcagaa gatcagcctg gtcagcggcc
    15121 gcctggcgcg gcggctgcgc gcgctcgggc tcgccagctt cggcgactac catcgcctgg
    15181 tgtcgagcgc cggcgcgcac gacgagttcg agcgcatggt cgacctgctc accacccacg
    15241 agacctattt cttccgcgaa cccaagcact tcgacttcct cgccgaaagc gtgctgcccg
    15301 cgctcgggcg cacgcgcacg ccgcgcttct ggagcgcggc gagctcgtcc ggcgaggagg
    15361 cctacagcct ggcgatggtg ctcgccgacc gtctcggcgg cgacgcgccc tgggaggtgt
    15421 tcggctcgga catcagccgc gaggtggtgg agaaggcgcg cggcggcgtc tatgcgatga
    15481 accgcatcga cggcatcccg cccgactacc tgcggcgcta ctgcctgcgc ggcgtaggca
    15541 gccgcgacgg tacgctgcag gtcgacgcgg cgctgcgccg acgggtgcgc ttcgcccagg
    15601 tcaatctcaa cggcaacctc gccggtgtcg gcgacttcga cgtcgtcttc ctgcgcaatg
    15661 tgctgatcta cttcgacctg ccgaccaagc gccgcatcgt cgaacgcatc gtgcgccaga
    15721 tgcggccggg tggctggctg ttcatcggcc actcggagag cctcaacggc ctcgacctcg
    15781 gcctgcgcca ggagcggccc accatctacc ggcgaatgtg agcatgcggc cggcttcgcg
    15841 caacacgatc gagatcttcc tgcagcccgg cgagttctgg ttcggcgacc gcgacacgcg
    15901 catccgcacc ctgctcggct cctgcgtgtc gatcacgatg tggcatccgc gcctgcgcat
    15961 cggcggcatg tgccacttca tgctccccga gcgcggcgtg cgtgcgtgcg ccgatggcct
    16021 ggacgggcgc tatgccgggg acgcgatcgc gctcttcctg cgcgagctgc gcgccgccca
    16081 cactcggccg caggattacc aggtcaagct cttcggcggc ggacgcatgt tccgcagccg
    16141 ccacgccgcg ggcgctcccg ccgcgctcga cgtcggcacg cgcaacgtcg aggccgcgcg
    16201 catgctgctg cgcacgcacg gctttcgcgt gcgcgccgcc cacgtcgagg gcgagggcca
    16261 ccgcaatctg atcttcgaca tcggcagcgg cgaggtctgg ctgcgccacg tcgctccggc
    16321 ggcggcgagc gccgcggtga tccacaccga agtcccctcc caagcccacc ccggagttca
    16381 cccggaatcc gcgagcgagg ccgcatgaac gcccccatcc gtgtcctcgt cgtcgacgac
    16441 tccgccgtgg tgcgccaggt ggtgagcgag atcctctccg ccgcacccgg catcgaggtc
    16501 gtcggcaccg cctccgatcc gctcttcgcc atggccaaga tgcgccgcga ctggcccgac
    16561 gtgatcaccc tggacgtgga gatgccgcgc atggacggca tcagcttcct gcgccagctc
    16621 atggccgagc gcccgacccc ggtcatcgtg tgctccacgc tcacctcgcg cggcgccgag
    16681 accaccatgc aggcgctcgc cgccggcgcc gtctccatca tcgccaagcc cacgctcggc
    16741 ctgaagggct tcctcaccga ctccgcccag gaactcgtcg ccgccgtgcg cgccgcggcg
    16801 cgtgcgaacc tgcgcgcgct acgggcgggg gcgggcgtcg gcgggggtgg tcccggcgtt
    16861 ggtggtcgag cagggtccgg agcggaaatc ggctccgcaa cggggcctgg cggagcgggc
    16921 gtggctgccg ctccgggcgt cggcgtggcg gccgcgcgcg gtgcagcggg cgccggaccg
    16981 gccgggtcgg gcgcgcgcgg cggatcgaac ggcgcgcccg gcggcgcgct cgcgcgcccg
    17041 gccgcggcga ccgcgatcgt gcccgccgcc gtggcgcccg cgcgcgccga ggccatggcc
    17101 gagaccaccg accgcatcat cgcgctcggc acctccaccg gcggcaccca ggccctcgag
    17161 gcggtgctgc gcggcctgcc gcgcaccgtg cccggcatcg tcgtggtgca gcacatgccg
    17221 gagaagttca ccgcgatgtt cgcccagcgc ctcgaccagg tgtgcgccct cgaggtgctc
    17281 gaggcccggg acggacagcg cgtcctcccc ggacgggtgc tcatcgcacc cggcgggcgc
    17341 cacatgacgg tgcggcgcag cggcgcgcaa tacctcgtcg aggtgcgcga cggcccgccg
    17401 gtgaaccgcc accgcccctc ggtcgacgtg ctcttccgct cggtcgcccg ctgcgccggg
    17461 cgcaacgcgc ttggcgtgat catgaccggc atgggcgacg acggcgcccg cggcctgcgc
    17521 gagatgttcg acgccggcgc ccagaccatc gcccaggacg aggccagctc cgtggtcttc
    17581 ggcatgccca aggaagccat ccgcctcggt gcggccaccc gcatcctcgc gctccaggcc
    17641 atcggccccg ccatcgagca atacacccgc gccagctgag cccccgcccc gcggtgcgac
    17701 gtttccgcag gagcgacgcg ggacggcgac cccccgccgc gcgtcctcgc cgtcccgcac
    17761 gcagcgatcg cgacttgcgt cgctcctacg gtgcggctgc gggtgcgcgc tggtttctgt
    17821 gggagcgacg cgagtcgcga tcgggcggtt cggcggcggt gggcggtggt ggctcgaagg
    17881 ccgcgatcgc gactcgcgtc gctcctacag tgcggctgcg ggtgcgcgct ggtttccgtg
    17941 ggagcgacgc gagtcgcgat tgggcggttc ggtggcggtg ggcggcggcg gctcgaaggc
    18001 cgcaatcgcg actcgcgtcg ctcctacggt gcggctgcgg gtgcgcgctg gtttctgtgg
    18061 gagcgacgcg agtcgcgatc gggcggttcg gcggcggtgg gcggtggtgg ctcgaaggcc
    18121 gcgatcgcga cttgcgtcgc tcctacggtg cggctgcggg tacgctggtt tctgtgggag
    18181 cgacgcgagt cgcggtcgtg tggttcggtg gcggtgctgg cgggggggcg gggggcgtgc
    18241 ttgaatgcag gatctctccg cgctttgccg ccccatgtcc aaggtcttca tctcctaccg
    18301 ccgcgacgac agcgccggct tcgccgggcg gctcgccgac gcgctcgaac gccgcttcgg
    18361 cgccgacagc gtgttccgcg atgtcgacga catcgccgca ggggccgatt tcggtgtggt
    18421 catcgagcag gccctgcagc aggtgcaggc ggtgctggtg gtgatcggta cgcgctggct
    18481 cgacgcgcgc gacgggcgcg ggcgtcggct cgacgatccc ggcgactacg tgcgccgcga
    18541 ggtcgagctc gcgctcgcct cggggaaacc cttgattccg gtgctggtcg gcggggcgac
    18601 gatgcccgcg gccgaggcgc tgccggcgac cctgcgcggg ctggccaacc gcaacgcgct
    18661 cgcgctcggc gatgccggct gggttgcgga tctcgcccgc ctggaggcgg cgcttgtgca
    18721 gtgggtggcg ccgcccgtgt cgcccgcacc ggtgcgtgga cggcgggcgc tcgcggccgc
    18781 gatcgcggcg ctggtcctcg cactcggcgg cggctggtgg tggctgcggg atggcgacgg
    18841 cgggctgcag gcgatcgtcg gcgcatggag cgccgaggtc gcctatccgt ggaacctgac
    18901 gctgcgcgag cattacgtgt tcacgctgga ggacggccgc atcggcggca gcgcgagctt
    18961 cctcggcgtg ccgcgcgcgc tggaggcggt ggcgtggggg gacgggcggc tgcgcttcct
    19021 cacccgcagc gagaccgtgt ccggcaacga gccgccgcgc gagatgggcc agctctacga
    19081 ggtctggcgc gagggcgaga cgctgcgcgt gcgcctgcag atccgccgca gccattcggt
    19141 cgatgcagca ctggagttca ccgcgcagcg ccaatgaggc cggctcagca ctgggctgca
    19201 cggcccgatc gcgactcgcg tcgctcccac ggcaagcacc gcgcttccgg agccgcatcc
//
LOCUS       DAOQBS010000052        19227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1987_length_19227_cov_16.289328, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000052 DAOQBS010000000
VERSION     DAOQBS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 19227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19227
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1987_length_19227_cov_16.289328"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PKN25_07675"
     CDS             complement(<1..874)
                     /locus_tag="PKN25_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV90814.1"
                     /translation="MRSIAAHAPTSLAFLDDIQLGRSRMSAPRAYRFLLALAAAATVS
                     SSHAAAIASQPFDIGVDMVQVFNDRLGAGRFFAGAGTDRLRVSARVTPSPDTDFYDQS
                     SNGGETAIFVTHPSTPGLDTRLQFVGLQSGGGGGKNDYTTTFNLANPFVQGQLAAWDA
                     TPFALRIENPSAPGGETTKWVTAPDYDSSVNLPFLQDVAITGGGLAPTLSWTVPHTTA
                     PITNIRIQLRRIDAEEPGRITAATLVHEAVLPLGSNGYTLDQAFSNGGLPGLPSGLEQ
                     GSKYEVAVILESAEP"
     gene            1062..2117
                     /locus_tag="PKN25_07680"
     CDS             1062..2117
                     /locus_tag="PKN25_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90815.1"
                     /translation="MKVTASSVELQSSHRFAQSLETRERLEVWRGTRDSGSAAESTQP
                     PVVALSAAGQNALAAESATAAVDAVAEEAVEDDPRLAMLIRMIEFLTGEPVRRFSMRD
                     LQSADAAESGYETGANPGTSGQAGARRAGFGLEYDFSSRFSETETIAFGAAGVVRTAD
                     GAEIRFELGFEMSRSYTESVAVSVRAGDQRLKDPLVLDFGGPAAALSEVRFDFDLDSD
                     GTREKLPLVSGSGFLAFDRNANGRIDDGRELFGPASGDGFSELARLDDDANGWIDEAD
                     AAWSQLRVWRPDAAGKGSVRSLSEAGVGALHLGRVATPFSLKGAANDTQGVMRASGVY
                     LREDGGVGTLSQVDLKV"
     gene            complement(2294..3970)
                     /locus_tag="PKN25_07685"
     CDS             complement(2294..3970)
                     /locus_tag="PKN25_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HNV90816.1"
                     /translation="MARTHPEGWRLLPAEGARGRELATLARLAAALPEDTTIYHSLHW
                     TRAEGRTAVFGEVDFAVVGPGGRVLLVEQQAGFLDETPTGLLRPGRRRAAQLSVELAR
                     NADALRARLRPLLDGTEPVLDVLLHCPDHCVRQPGTAGLDPERIVDAGRRDQLPAIVA
                     ALTRPQEGDPALDAAHRQALHRFFADLLELVPDVQSHIGQVEDLTTRLSGGLTEWARR
                     IVVEPHRLRVTATAGSGKTQLALAAYTDALAAGRRPLYVCYNRPLADHFARIAPAGGE
                     IATYHQLCDRRVRDAGRKPAFGAPGAFRRMEADFATLVREQPDPAWQFDELIIDEGQD
                     FTEPWRDALLALLKPAGRAWWLEDPLQNLYDRPPVALPGWVGLSADTNYRTPADVLAL
                     LNARLPLPAPVRAGSPVTDSEPEIFAWRDEAGLMDATKRAITRALGLGFRRDAIVLLT
                     FRGREHSRFTPLDHLGPHRLRAFTGRYDLLGEPEHSQGELLIDSVYRFKGQSAPCILF
                     TEIDFETTGGRMDELTMRKLFVGATRATMKLILVASERAAAALAPVAEAG"
     gene            complement(4185..>4360)
                     /locus_tag="PKN25_07690"
     CDS             complement(4185..>4360)
                     /locus_tag="PKN25_07690"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNV90817.1"
                     /translation="GRAQGSASVLLRLLNRRFGPLSPDIIRRLSQSTPEQLEIWAERV
                     LEARTIDEVFAGN"
     assembly_gap    4361..4460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4461..5279)
                     /locus_tag="PKN25_07695"
     CDS             complement(<4461..5279)
                     /locus_tag="PKN25_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosolic protein"
                     /protein_id="HNV90818.1"
                     /translation="MPASAAQDDYDSPWKEAVEHAFPEFIDFYFPDAGRQIDWARGHR
                     FLDKELQQIVRDAALGRRHVDKLASVTTHAGEEDWLCVHIEVQGSMDPDFARRMFVYN
                     YRIYDSYDRPVASLAVLADDDPAWRPDRFGYERLGCRHNLQFPVAKLVDHAADEAALL
                     CNPNPFALVTAAHLYTRRTRRSPIARFDAKRRLVRLLYERDWTRQRILDFFSVLDWMM
                     RLPREFEQRLWQDIENIEGERKVKYVTSVERLAIERGLQKGMEQGLEIGIEKGIE"
     gene            5459..6052
                     /locus_tag="PKN25_07700"
     CDS             5459..6052
                     /locus_tag="PKN25_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNV90819.1"
                     /translation="MPLTLAAPVHSELIIKKSRFIGCVQPMSDRAGAQKVVAALWAQH
                     PGARHVCWALLAGGQSAAVDDGEPSGTAGRPMLDVLRHQDLEGVLATVVRYFGGVKLG
                     AGGLVRAYTDAVAQALLEAEKVPIVRLATLRCVVPYAMEGLLRRELDAAGASLLAVAH
                     GQAVELCFSLPEDAAAALRARLAEAGQGRIEWIAEVE"
     gene            complement(6279..7049)
                     /locus_tag="PKN25_07705"
     CDS             complement(6279..7049)
                     /locus_tag="PKN25_07705"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HNV90820.1"
                     /translation="MNKTINLNADLGESFGPWTMGEDEALLQVVRSANIACGFHAGDP
                     VVMRHTVRTALAAGVSLGAHPAYPDLQGFGRRPMKMAPAELEAMIIYQVGALAGMAAA
                     EGGRITHVKPHGALNNQACEDAALADAVARAVKAIDPGLILLAPVLSELHAAGARAGL
                     RVAGEVFADRAYTDKGTLVPRGQPGAVIHDHDELVTHVLRMLEAGGVVSQSGKVLPTS
                     MDSICVHGDTLGAVESARHLRTELEARGWTIAPLPQLS"
     gene            complement(7144..8196)
                     /locus_tag="PKN25_07710"
     CDS             complement(7144..8196)
                     /locus_tag="PKN25_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HNV90821.1"
                     /translation="MSTRTEVEILSPGAFASIQDGGRRGHRRIGVPWAGVLDRRLMRI
                     ANALAGRAEDAAVIECFDGGLHVAASGGAVKLAVAGDAVVEVEGTEGRRQLAPWRSVT
                     LADGEQLRIRKMEGGRIAMVAIVGLEPAAVMGSASTYARAGIGGVDGRALGPGTRLAL
                     SADADPWDSDRVLAQPPAADTGPIRLVPGPQADHFSPTALDALVGGEYRVTTEADRMG
                     IRLEGAQLEHAGAAEIVSDATVPGSIQVPGAGQPIVLLADAQTAGGYPKIATVIGADL
                     GRLAALRPGQSLRFAAVSAAEGACIARAAETETRALIASIRALPPDGIDLMALYTGNL
                     VDGVVHALGTEYRPLY"
     gene            complement(8193..8954)
                     /gene="pxpB"
                     /locus_tag="PKN25_07715"
     CDS             complement(8193..8954)
                     /gene="pxpB"
                     /locus_tag="PKN25_07715"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HNV90822.1"
                     /translation="MSFRILDAGDAALTIEFGSIIDPALLAEVNALDAAVLRRKQAGE
                     LPGVIETMPTFRSLTVFFDPLVTDRDAVLATLQPLIAAAEHGSTTDGRHWRLPVCYEG
                     EAAPDLAEVAGAIGIGEDEVVALHSGAEYLVYMIGFLPGFPFMGDLPAPLRLPRRAQP
                     RVRVPAGSVAIATGLTAIYPWESPGGWHLLGRCPVPLFDARRTSPSLLAAGDRVRFVP
                     VSAQECRAIEAGLASAEIDPMQWLESAPTPAENAA"
     gene            complement(8985..9500)
                     /locus_tag="PKN25_07720"
     CDS             complement(8985..9500)
                     /locus_tag="PKN25_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HNV90823.1"
                     /translation="MKEASRIVSSQHLISPKSPELSEMEFGLIIAWHAFARWMMRCMT
                     TAGVKDMTPIDVLVLHHVSHRDSEKRLADICFVLNVEDTHVVSYSLRKLAGLGLVQSV
                     KKGKEAFFSVTERGREICLAYRDVREACLMPGFTGSPEDNAQIGELARLLRTLSGRYD
                     QAARAASTSSL"
     gene            9683..10672
                     /locus_tag="PKN25_07725"
     CDS             9683..10672
                     /locus_tag="PKN25_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNV90824.1"
                     /translation="MRFPRPLVKVIATSLVGLGLAAVAHAQTAWDMPTPYPANNFHTE
                     NIQQFAKEVDEATGGKLKITVHANGSLFKANEIKRAVQSGQAQIGELIISGLANEDAL
                     YALDTVPFLATGYADSKKLWQASKAAVEARLAKNGLKILYSVAWPPQGLYSMTELKSI
                     GDLKGMKIRSYSPTVARMIELMGGQPVTVQAADLTQALSTGVVSANLTSSATGYDTKS
                     WENLSHYYDIQAWLPKNIVFVSKKAFDALDQPSRDALLKAAAAAEERGWKVSEEKTAW
                     YIDQLKANKMKVQPAPEALQAELLKVGEKMTAEWVEKAGPDGQAILDAYRKLK"
     gene            10731..11249
                     /locus_tag="PKN25_07730"
     CDS             10731..11249
                     /locus_tag="PKN25_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNV90825.1"
                     /translation="MRAFLDSLYRLSGALAAVGMVATLVLVATGVFTRPLGIYLPGTD
                     DYAGYAMAACGFLALAYTFKHGEHIRVSLVLERVGPRLRAALEWFSLAAGTAVAAMLA
                     FYAVRMVLQSHEFEEISQGVDATPLWIPQLSMAFGSVVLLIALIDDLVMTTLGRAPAR
                     LATHSGEAARME"
     gene            11262..12560
                     /locus_tag="PKN25_07735"
     CDS             11262..12560
                     /locus_tag="PKN25_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNV90826.1"
                     /translation="MEVMIAAVLIGLMLALLAGGVWIGLALMAIGILGMLGFTPRAPG
                     DGMAVAIWSHGSSWTLTALPLFLWMGEILFRTKLSEDMFKGLSPWLERLPGRLLHTNI
                     IGCTLFAAVSGSSAATCATIGKIALPELQRRGYPESMALGTLAGAGTLGLLIPPSIIM
                     IVYGVAADVSISKLFIGGVLPGILLASLFMGWVVVWSLMNPGKIPAADLKTDFMGKLR
                     ASKNLIPVILLIGGVLGSIYSGIATATEAAAIGVIGSLIIAASQRTLNRKSFFGALMG
                     ATRLYCMIALILAGSAFLTLAMGYIGLPRHLAEWIGGLGLTPGMLLVALAIFYILLGC
                     FLDGISIVVLTMAVLMPTIQAAGIDPLWFGIFVVVVVEMAQVTPPVGFNLFVLQGLTG
                     RDMTVIARYALPYFLLMVLAVVLLYTFPGLVTWLPGHMIG"
     gene            complement(12589..13551)
                     /locus_tag="PKN25_07740"
     CDS             complement(12589..13551)
                     /locus_tag="PKN25_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNV90827.1"
                     /translation="MATSAPSQIHTRLRVFLAVARHLSFTRAGEELSITTGAVSQQIK
                     LLEDWLAQRLFRRLPRRLELTDAGKRLLAAVDPAYAAVELAIGRLRGGALSGVVRVRT
                     LPSFLATWLVPRLPRLMARFPQIELQVEAEDSTYSLRDGEFDLAIDLNDGSYPGFQST
                     VLLEEEIFPVCSPRLLEGRPPLLGAEDLRHYPLLHDMTAWRGSPPYAEWERYLKAIGA
                     PQVEVRRGYMFNRNHITMQCAIAGMGVAIARRTLITDELDSGRLVAPFAQSVRTGKRY
                     CVIHSAGALADPRVATVHDWLAAEARAAELAAGPCADTAPAPGD"
     gene            13694..15073
                     /locus_tag="PKN25_07745"
     CDS             13694..15073
                     /locus_tag="PKN25_07745"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HNV90828.1"
                     /translation="MSISVLDLFKIGVGPSSSHTVGPMRAAHDFVAELQRRGLLDQVR
                     RIEAKLYGSLSATGVGHGTDRAVVAGLMGARPDEVDPDFLVGAVDAVRIDASLPLGGT
                     TAVPFEWTRDLRLLPVSLPYHPNAMRLAAHGEAGVLYENTYYSVGGGFVVDEAQAQSG
                     APAEAAVAVPYPFDTGAELLAQCRRHGLRISELMLANESAWRSEAETRAALLRIWDVM
                     RACVTRGVGHEGVLPGGLNVRRRAPGLYRKLSEQASGRNMISDSMSALDWVNLYALAV
                     NEENAGGGRVVTAPTNGAAGIIPAVMHYYMKFMPGSDERDVENFLLAAAAVGVLCKKN
                     ASISGAEVGCQGEVGSACAMAAAGLAEVLGGTPEQVENAAEIGLEHNLGLTCDPVGGL
                     VQVPCIERNAMASIKAINAAQLALRGDGAHTVSLDQVIRTMRDTGRDMLDKYKETSRG
                     GLAVSFPEC"
     gene            15208..16446
                     /gene="sstT"
                     /locus_tag="PKN25_07750"
     CDS             15208..16446
                     /gene="sstT"
                     /locus_tag="PKN25_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HNV90829.1"
                     /translation="MTTANPTALQRVMRMSLISQIAIGLVLGIALALIAPGATKSVAL
                     LGDLFIAALKAVAPILVFVLVAAAIANHRHGQPTHMRPILVLYLLGTFAAAVVAVIAS
                     FAFPTTLVLDTAAATGNPPGGILGVLKNLLLSVVSNPVRALMEANFIGILAWAIGLGV
                     ALRHAAESTRKMVNDVADAVSSIVQVVIRFAPLGIFGLVAATMGESGFDALLGYGKLL
                     MVIVGCMVFVALVVNPLFVFWKLRTNPYPLVFTCLRESGVTAFFTRSSAANIPINLEL
                     CKRMGLHEDTYSISIPLGATINMAGAAITISVMALAAAHTLGIATDFPTALLLCLVSA
                     LAACGASGVAGGSLLLIPMATSLLGVQADVAMQVVAIGFVISVVQDSCETALNSSTDV
                     LFTAAACAAESGEPLQLDRT"
     gene            16600..17856
                     /locus_tag="PKN25_07755"
     CDS             16600..17856
                     /locus_tag="PKN25_07755"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNV90830.1"
                     /translation="MNKNPATAVEQSGKPQSPARERFLAHLHGVVDQIRADGFYKAER
                     VIDSPQGAQVHLGTGADVLNFCANNYLGLANDPRLVKAAQDGLAEDGFGMASVRFICG
                     TQSVHKQLEASISKFLGTEDTILYGSCFDANGGLFETLLTEEDAVISDELNHASIIDG
                     VRLCKARRYRYRNNDMADLEAQLQAADAAGVRFKLIATDGVFSMDGVIADLKSVCDLA
                     DKYGALVMVDDSHAVGFVGTHGRGTAEYCGVEGRVDIITGTLGKALGGSSGGYTSGHK
                     EIIELLRQRSRPYLFSNTLAPSIAAASIKVMELLASDEGLELCRRVKENGEHFRREMT
                     KLGFTLAGKDHPIVPVMLGDAQLAGRMAEELLKEGIYVIGFSFPVVPKGKARIRTQMS
                     AAHTTAQIDRAVAAFAKVGKALGAIA"
     gene            17890..18927
                     /gene="tdh"
                     /locus_tag="PKN25_07760"
     CDS             17890..18927
                     /gene="tdh"
                     /locus_tag="PKN25_07760"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNV90831.1"
                     /translation="MKALAKLTPSVGLTLTSVEKPEVGPNDVLIKIKKTAICGTDIHI
                     WKWDEWAQKTIPVPMHVGHEYVGVIAEMGQEVRGFKIGDRVSGEGHITCGFCRNCRAG
                     RRHLCRNTVGVGVNRAGAFAEYLAIPAVNAFKIPDDISDDLAAIFDPFGNATHTALSF
                     NLVGEDVLITGAGPIGIMAVAIARHVGARHVVITDVNDHRLELARKMGATRAVNVSRE
                     NLADVKTELHMVEGFDVGLEMSGVPSAFTSMLEHMNHGGKIALLGIPPSNTAIDWNQV
                     IFKGLEIKGIYGREMFETWYKMVAMLQSGLDLSPIITHHYPVEEFKTGFETMLSGNSG
                     KVILDWEALAA"
     gene            complement(18994..>19227)
                     /locus_tag="PKN25_07765"
     CDS             complement(18994..>19227)
                     /locus_tag="PKN25_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HNV90832.1"
                     /translation="DYCKGCAYDPRRRHGASGKPACPFNSLYWDFLLRHEARFARNPR
                     MAMPYKAWAKMDAGERAATLAQAAHWLARLDEL"
BASE COUNT         2843 a         6413 c         6860 g         3011 t
ORIGIN      
        1 cgggttcggc ggattcgagg atcaccgcca cttcgtactt gctcccctgc tccaggccgc
       61 tcggcagccc gggaagcccg ccgttgctga aggcctgatc cagggtgtag ccgttgctgc
      121 ccagcggcag gacggcctcg tgcacgagcg tcgccgcggt gatgcgcccg ggctcctcgg
      181 cgtcgatgcg ccgaagctgg atacggatgt tggtgatcgg cgcggtggtg tgcgggacgg
      241 tccacgacag ggtgggggcg aggccgccgc cggtgatcgc gacatcttgc aggaagggca
      301 ggttgacgct gctgtcgtag tccggggctg tgacccactt cgtggtttcg ccgccggggg
      361 cggacgggtt ctcgattcgc aacgcgaacg gggtcgcgtc ccaggcggcg agctgcccct
      421 gtacgaaggg attggcgaga ttgaaggtcg tcgtgtagtc gttcttgccg ccgccgccgc
      481 cggattggag gccgacgaac tgcaggcggg tatcgagccc aggagtggag gggtgcgtga
      541 cgaagatcgc ggtctcgccg ccgttcgagc tctgatcgta gaagtccgta tccggcgagg
      601 gggtcaccct tgcgctcacg cgcaggcggt ccgtacccgc accggcaaag aagcggccag
      661 cgccgagccg gtcgttgaat acctggacca tgtcgacacc gatgtcgaag ggttgcgagg
      721 cgatggctgc tgcgtggctg ctggagacgg tcgccgcggc ggcgagggcg agcagaaatc
      781 gataggctct tggggcggac attcgtgatc tcccgagctg gatgtcatcg aggaatgcga
      841 gggatgtcgg tgcgtgggct gcaatcgagc gcatattcaa tatagaacgt gttcatgaat
      901 gctcaacgag tttctgatgc acctcgaata ccttcgcccg tcgacggcgc ggcgcgggcg
      961 tcggtccccg cagatggact tcgaggcggc cgatcgcggt caaggaacgt gacgggatgt
     1021 cgttaggtgt accggtcagt cacctttgcg aggccttaga catgaaagtc accgcgtcca
     1081 gtgtcgagct ccagtcctcg caccgtttcg cccagtcgct ggagacccgt gagcgcctcg
     1141 aagtctggcg aggcacgcgc gattccggct ccgcagcgga gagcacgcag cccccggtgg
     1201 tggcgctctc cgccgcgggc cagaacgccc tggcggccga gagcgcgact gcggcggtgg
     1261 acgcggtcgc cgaggaggcg gtcgaagacg atccgcgcct cgccatgctg atccgcatga
     1321 tcgagttcct caccggcgag ccggtgcggc gcttcagcat gcgcgacctg cagtctgccg
     1381 acgccgccga gtctggctat gagaccggag caaacccggg tacgagcggc caggctggcg
     1441 cacggcgcgc cggcttcggg ctcgaatacg acttcagcag ccgcttcagc gagaccgaga
     1501 cgattgcctt cggtgccgcc ggcgtggtgc gcaccgcgga cggtgccgag atccgtttcg
     1561 agctcggctt cgagatgtcg cgcagctaca ccgagtcggt ggcggtcagt gtgcgcgccg
     1621 gcgaccagcg cctgaaggat ccactggtgc tcgacttcgg cggccccgcg gcggcgctct
     1681 cggaggtgcg cttcgacttc gatctggata gcgacggcac cagggagaag cttccgctgg
     1741 tgagcggcag cggcttcctc gccttcgacc gtaacgccaa tggccgcatc gacgatggcc
     1801 gcgagctctt cggacccgcc agcggcgacg gcttttccga actcgcccgc ctcgacgacg
     1861 atgccaacgg ctggatcgac gaggccgatg ccgcctggtc gcagttgcgc gtgtggcggc
     1921 cggatgcggc gggcaagggc agcgtgcggt cgctgagcga ggcgggcgtc ggcgccctcc
     1981 acctcggccg cgtggcgaca ccgttcagcc tgaagggcgc tgcgaacgac acccagggtg
     2041 tgatgcgcgc gagcggcgtc tacctgcgcg aggacggtgg ggtcggcacg ctgagccagg
     2101 tcgacctgaa ggtctgagca cgcagcggcg cggcatcctg cgtcgcagcc cgacggccgc
     2161 tcgcgactcg cgtcgttcct gcagcgcggc tccaggaacg tggtgttttt cgcaggagcg
     2221 acgcgagtcg cgatggcggc ggttgggtag cgctacgttt cccggagacc cgccgcgttc
     2281 ggggggcggc gcctcagccc gcttccgcga ccggcgcgag cgcggccgcc gcacgctccg
     2341 acgccaccag gatcaacttc atcgtcgccc gcgtggcgcc gacgaaaagc ttgcgcatgg
     2401 tgagctcgtc cattcgcccg cccgtcgtct cgaagtcgat ctcggtaaac aggatgcagg
     2461 gcgccgactg gcccttgaag cgatacaccg aatcgatcag cagttcgccc tgcgagtgct
     2521 ccggctcgcc gagcaggtcg tagcggccgg tgaaggcgcg caggcggtgg gggccgaggt
     2581 ggtcgagcgg ggtgaagcgc gaatgctcgc ggccgcggaa ggtcagcagc acgatcgcgt
     2641 cgcggcggaa gcccaggccg agggcgcgcg tgatcgcgcg tttggtggca tccatcagcc
     2701 cggcttcgtc gcgccaggcg aagatctccg gttcgctgtc ggtcaccggg ctgccggcgc
     2761 gcaccggggc gggcagtggc aggcgggcgt tgaggagtgc gagcacgtcg gccggggtgc
     2821 ggtagttggt gtcggcgctc agccccaccc agccgggcag cgccaccggc ggacggtcgt
     2881 agaggttctg cagcgggtct tccagccacc aggcgcgccc cgccggcttc agcagcgcga
     2941 gcagggcatc gcgccagggc tcggtgaagt cctggccctc gtcgatgatg agctcgtcga
     3001 actgccaggc gggatcgggc tgctcgcgca ccagggtggc gaagtccgcc tccatgcggc
     3061 ggaaggcgcc gggcgcgccg aaggccggct tgcggccggc gtcgcgcacg cggcggtcgc
     3121 agagctggtg gtaggtggcg atctcgccgc cggcaggcgc gatacgcgcg aagtggtcgg
     3181 cgagcggacg gttgtagcac acgtagagcg ggcgccggcc ggcggcgagc gcgtcggtgt
     3241 aggcggcgag cgcgagctgg gtcttgccgc tgccggcggt ggcggtcacg cgcaggcggt
     3301 gcggctcgac cacgatgcgg cgcgcccact cggtgagccc gcccgacagc cgggtggtga
     3361 ggtcttcgac ctggccgatg tgcgactgca cgtccggcac cagctcgagc aggtcggcga
     3421 agaagcggtg gagggcctgg cggtgcgcgg cgtcgagcgc cgggtcgccc tcctgcggcc
     3481 gcgtcagcgc ggcgacgatc gcggggagct ggtcgcgccg gccggcgtcg acgatgcgct
     3541 cggggtcgag tccggcggtg ccgggctggc gcacgcagtg gtcggggcag tgcagcagca
     3601 cgtcgagcac gggctcggtg ccgtcgagca gcgggcgcag gcgggcgcgc agggcgtcgg
     3661 cgttgcgcgc gagctcgacg ctgagctgcg ccgcgcggcg cctgccgggg cgcagcaggc
     3721 cggtaggcgt ctcgtcgagg aagccggcct gctgttcgac cagcagcacc cgcccgcccg
     3781 ggccgaccac ggcgaaatcg acctcgccga acaccgccgt gcgaccctcg gcgcgcgtcc
     3841 agtgcaggct gtggtagatc gtggtgtcct cgggcaacgc ggcggcgagg cgggcgaggg
     3901 tggcgagctc gcgcccgcgc gcgccctccg cggggagcag gcgccagcct tcggggtggg
     3961 tgcgggccat ggtgggcctc agtcgatggg ctcgaacagg cgggcgacga tgcgctggca
     4021 agacgggagc tcgaaggcct cggggttcat gtcgagaatg taatggcgtt catccgcttt
     4081 cacggttgcg gggcgaagtg gccggatcgc ttcacccatc ggagaccggc ggggttgcgg
     4141 cggtttgcgc acgccgctca ccccgtccag cgtgagcccg tgcttcagtt ccccgcgaac
     4201 acctcgtcga tcgtccgcgc ctcgagcacg cgctcggccc agatctcgag ctgctcgggc
     4261 gtggactggg agagacgccg gatgatgtcg ggggagagcg ggccgaagcg ccggttcagc
     4321 aggcgcagga ggacgcttgc actgccttgg gcacgaccct nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn ctcgatcccc ttctcgatac cgatttcgag cccttgctcc
     4501 atgcccttct gcagcccgcg ctcgatcgcg agtcgttcga cgctggtgac gtacttcact
     4561 ttgcgttctc cttcaatatt ttcaatatct tgccacagtc tctgctcgaa ctccctgggt
     4621 agtcgcatca tccagtcgag caccgaaaag aagtcaagga tgcgttgtcg cgtccagtcg
     4681 cgttcataga gtagtcgcac caggcggcgc ttggcgtcga agcgggcgat cgggctgcgg
     4741 cgggtgcgac gggtgtagag gtgcgcggcg gtgacgagtg cgaaggggtt cgggttgcac
     4801 agcagcgcgg cttcgtccgc tgcgtggtcg accagcttgg cgaccggaaa ttgcagattg
     4861 tgccggcagc cgaggcgctc gtagccgaag cggtcggggc gccaggccgg gtcgtcatcg
     4921 gcgagcacgg ccaggctggc gaccggacgg tcgtagctgt cgtagatgcg gtagttatag
     4981 acgaacatcc ggcgtgcgaa gtcggggtcc atgcttccct ggacctcgat gtgcacacac
     5041 agccagtcct cctcgccggc gtgcgtggtg acgctggcga gcttgtcgac atggcgccgg
     5101 ccgagcgcgg cgtcgcgtac gatctgctgc agctccttgt cgaggaagcg gtggccgcgc
     5161 gcccagtcga tctggcggcc ggcatcgggg aagtagaagt cgatgaactc cgggaaggcg
     5221 tgctcgacgg cctccttcca cggactgtcg taatcgtcct gcgcggcgct ggcgggcatt
     5281 gcgagctcct gggtgaggga gctggaatgc tatttggaaa tatgacaaaa gggaattggg
     5341 ggcattggga tgcgtccccg aattttcgga gtatccgcga ttttcggggg ctgcacgcca
     5401 tggcaatgcc ggctggccgc cgccgtgccg ccgcgcgaat cggactagaa tcccccccat
     5461 gccgctgacc ctcgccgctc ccgtccacag cgaactgatc atcaagaaga gccgcttcat
     5521 cggctgcgtg cagccgatga gcgaccgcgc cggcgcgcag aaggtcgtgg ccgcgttgtg
     5581 ggcgcagcat ccgggggcgc gccatgtgtg ctgggcgctg cttgcgggcg ggcagtcggc
     5641 ggcggtggac gacggcgagc cgagcggcac cgccgggcgg ccgatgctcg acgtgctgcg
     5701 ccaccaggac ctggagggcg tgctcgccac cgtggtgcgc tacttcggcg gcgtgaagct
     5761 gggcgccggg ggtctggtgc gcgcctacac ggacgcggtg gcgcaggcct tgctcgaggc
     5821 cgagaaggtg ccgatcgtgc gcctggcgac gctgcgctgc gtggtgccgt atgcgatgga
     5881 ggggctgctg cggcgcgagc tcgacgccgc cggggcgagc ctgctcgcgg tggcgcacgg
     5941 gcaggcggtg gagctgtgct tcagcctgcc cgaggatgcc gccgcagccc tgcgcgcgcg
     6001 ccttgcggag gcggggcagg gccggatcga gtggatcgcg gaggtcgagt gagcgttggg
     6061 cgccaagggg cggcagcgtc cgggtttgcg cgggggcgag gatcgcgact tgcgtcgctc
     6121 ctacagggcg cctggggcgt agggtgtggt cgtaggagcg acgcaagtcg cgatcgaatc
     6181 atccggagtc ctccccccgg cgtatccggc gcatgcgcag atcaagcgcc gaaatcgcga
     6241 cttgcgtcgc tcctgcctga tccgccggcg ggtcggcctc aggagagctg tggcaggggg
     6301 gcgatggtcc agccgcgcgc ttcgagttcc gtgcgcagat gtcgcgcgct ctccactgcg
     6361 cccagggtgt cgccgtgcac gcagatgctg tccatcgaag tcggcagcac cttgccgctc
     6421 tggctgacca cgccgccggc ctccagcatg cgcagcacgt gggtgacgag ctcgtcgtgg
     6481 tcgtggatga ccgcgccggg ctggccgcgc ggcaccagcg tgcccttgtc ggtgtaggcg
     6541 cggtcggcga agacctcgcc ggcgaccctc agcccggcgc gcgcgccggc cgcatggagt
     6601 tccgacagca ccggggcgag caggatcagc ccggggtcga tggccttgac cgcgcgcgcg
     6661 accgcgtcgg cgagcgccgc atcctcgcag gcctggttgt tgagtgcgcc gtgcggcttg
     6721 acgtgggtga tgcggccgcc ttcggccgca gccatgccgg cgagcgcgcc gacctggtag
     6781 atgatcatcg cctcgagctc ggccggcgcc atcttcatcg gccggcgacc gaagccctgc
     6841 aggtcggggt aggccgggtg ggcgccgagg ctcacccccg ccgcgagcgc cgtgcgcacg
     6901 gtgtggcgca tcaccaccgg gtcgccggcg tggaagccgc aggcgatgtt ggccgagcgc
     6961 accacctgca gaagggcctc gtcctcgccc atggtccagg ggccgaagga ttcgccgagg
     7021 tcggcgttga ggttgatcgt cttgttcatg ggcggggctc cttggggtgg ggagatcggg
     7081 gcgtgcaggc tgggggccgg gattgcgact tgcgtcgcgc cagggcgggc ggcgcttgcg
     7141 cgatcaatac agcggtcggt attcggtgcc gagggcatgc acgacgccgt cgaccaggtt
     7201 cccggtgtac agcgccatca ggtcgatgcc atcgggcggc agggcgcgga tcgaggcgat
     7261 caacgcccgg gtctcggtct ctgcggcgcg cgcgatgcac gcgccctcgg cggcgctcac
     7321 ggcggcgaag cgcaggctct ggccggggcg cagcgcggcg agacggccga ggtcggcgcc
     7381 gatcacggtg gcgatcttgg gatagccgcc ggcggtctgc gcgtcggcga gcagcacgat
     7441 gggctggccg gcaccgggca cctggatgga gccgggcacg gtggcgtcgg agacgatctc
     7501 ggcggcgccg gcgtgctcca gctgcgcgcc ctcgaggcgg atgcccatgc ggtcggcctc
     7561 ggtggtgacg cgatactcgc cgcccaccag ggcgtcgagc gcggtggggc tgaagtggtc
     7621 ggcctgcgga ccgggcacca ggcggatcgg accggtgtcg gccgcgggcg gctgggcgag
     7681 cacgcggtcg ctgtcccagg ggtcggcgtc ggcggagagc gccaggcgcg tgccagggcc
     7741 gagcgcacgg ccatccacgc cgccgatgcc ggcgcgcgca taggtcgagg cgctgcccat
     7801 caccgccgcc ggctcgaggc cgacgatcgc gaccatggcg atgcgtccgc cctccatctt
     7861 gcggatgcgc agctgctcgc cgtcggccag cgtcaccgag cgccacggcg cgagctggcg
     7921 ccggccctcg gtgccttcga cctcgaccac cgcgtcgccg gccaccgcga gcttcaccgc
     7981 gccgccgctc gcggcgacgt gcaggccgcc gtcgaagcat tcgatcaccg cggcgtcctc
     8041 ggcgcgaccg gcgagcgcat tggcgatgcg catcaggcgg cggtcgagca cgcccgccca
     8101 gggcacgccg atgcggcggt ggccgcggcg gccgccgtcc tggatggagg cgaaggcgcc
     8161 gggggagagg atttccactt cggtccgggt gctcatgctg cgttctccgc gggggtcggg
     8221 gcgctttcca gccattgcat ggggtcgatt tcggcgctgg cgaggccggc ctcgatggcg
     8281 cggcattcct gggcgctcac cgggacgaag cgcacgcggt ctccggccgc gagcagcgag
     8341 ggcgaggtgc ggcgggcgtc gaacaacggc accgggcagc gcccgagcag gtgccatccg
     8401 ccggggctct cccaggggta gatcgcggtc aggccagtgg cgatcgccac gcttccggcg
     8461 ggcacgcgca cgcgcggctg ggcgcggcgt ggcaggcgca gcggcgcggg caggtcgccc
     8521 atgaagggga agccgggcag gaagccgatc atgtagacca ggtattccgc tccgctgtgc
     8581 agcgcgacga cctcgtcctc gccgatgccg atcgcgcccg ccacctcggc gaggtcgggg
     8641 gcggcctcgc cctcgtagca caccggcagg cgccagtggc ggccgtcggt ggtgctgccg
     8701 tgctcggcgg cggcgatcag cggctgcagc gtggcgagca ccgcatcgcg gtcggtgacg
     8761 agcgggtcga agaacacggt cagcgagcgg aaggtgggca tggtctcgat cacgcctggc
     8821 agttcgcccg cctgtttccg gcgcagcacg gcggcgtcga gcgcattgac ctcggccagc
     8881 agggcagggt cgatgatgct gccgaattcg atggtgagcg cggcgtcgcc ggcgtcgagg
     8941 atgcgaaagc tcacgggagg gctccctggt gatgtgcagc ggatttacag cgacgaagtg
     9001 gaggccgcgc gcgcggcctg gtcgtagcgg ccggacaggg tgcgcagcag gcgggcgagt
     9061 tcgccgatct gggcgttgtc ctcggggctg ccggtgaagc cgggcatcag gcaggcctcg
     9121 cgtacgtcgc ggtacgccag gcagatctcg cggccccgct cggtgacgga gaagaacgcc
     9181 tccttgccct tcttgacgct ctgcaccagg ccgaggccgg cgagcttgcg cagcgagtag
     9241 gagacgacgt gggtgtcctc gacgttgagc acgaagcaga tgtccgcaag gcgcttctcg
     9301 gagtcgcggt gcgacacgtg gtgcagcacc agcacgtcga tcggcgtcat gtccttgacg
     9361 ccggcggtgg tcatgcagcg catcatccag cgtgcgaaag catgccaggc gatgatgagg
     9421 ccgaactcca tctcggaaag ttcgggcgat ttcggcgaga tcaggtgctg ggaggacacg
     9481 atgcggctgg cttctttcat gactgcctcc gggggtggaa gacaaggttt cgcggacgtt
     9541 ttgcaaaaaa aacgtgtacg ttttgttgac aagataccca caaaatctct attgtgcaat
     9601 aacggtgact tatcgaagac cggtcgccgc ccgccaatcc gcgttccggg cttctcgccc
     9661 tcctctacca ggagtaatcg ccatgcgctt cccgcgtccc ctcgtcaagg tgatcgccac
     9721 ttcgctcgta ggcctgggtc tggccgccgt cgcccacgcc cagaccgcgt gggacatgcc
     9781 cacgccctac ccggccaaca acttccacac cgagaacatc cagcagttcg ccaaggaggt
     9841 cgacgaggcc accggcggca agctcaagat caccgtgcac gccaacggct cgctgttcaa
     9901 ggccaacgag atcaagcgcg cggtgcagtc cgggcaggcg cagatcggcg agctgatcat
     9961 ctccgggctg gccaacgagg acgcgctcta cgcgctcgac accgtgccct tcctggccac
    10021 cggctatgcg gattcgaaga agctgtggca ggcctcgaag gcggcggtcg aagcgcgcct
    10081 ggcgaagaac ggcctcaaga tcctgtactc ggtggcctgg ccgccgcagg gcctgtactc
    10141 catgaccgag ctcaagagca tcggcgacct caagggcatg aagatccgct cctacagccc
    10201 gaccgtggcg cgcatgatcg agctgatggg ggggcagccg gtgaccgtgc aggccgccga
    10261 cctcacccag gcgctgtcga ccggcgtggt gtcggccaac ctgacctcct cggccaccgg
    10321 ttacgacacc aagagctggg aaaacctgag ccactactac gacatccagg cctggctgcc
    10381 gaagaacatc gtgttcgtgt cgaagaaggc cttcgacgcg ctcgaccagc cgtcgcgcga
    10441 tgccctgctg aaggccgccg ccgcggccga ggagcgcggc tggaaggtgt cggaagagaa
    10501 gaccgcctgg tacatcgacc agctcaaggc caacaagatg aaggtccagc ccgcgccgga
    10561 ggcgctgcag gccgagctgc tcaaggtcgg cgagaagatg accgccgagt gggtcgagaa
    10621 ggccggtccg gacggccagg ccatcctcga cgcctaccgc aagctgaagt gaccggcgga
    10681 tgccgcatcg gccccgccgt ggcggggcct tcttgttcag gagatcgccc atgagagcgt
    10741 tcctcgattc cttgtaccgc ctgtccgggg cgctcgccgc ggtcggcatg gtggccacgc
    10801 tggtgctggt cgcgaccggc gtcttcacgc gcccgctcgg catctacctg cccggcaccg
    10861 acgactacgc cggctacgcg atggcggcct gcggcttcct cgcgctggcc tacaccttca
    10921 agcacggcga gcacatccgc gtgagcctgg tgctcgagcg cgtcggcccg cgcctgcgcg
    10981 cggcgctgga gtggttctcg ctcgccgccg gcaccgcggt ggcggcgatg ctggccttct
    11041 acgccgtgcg catggtgctg cagtcgcacg aattcgagga aatctcgcag ggcgtggacg
    11101 ccacgccact gtggattccg caactgtcga tggccttcgg ctcggtcgtg ctgctgatcg
    11161 cgctgatcga cgacctggtg atgaccaccc tggggcgcgc accggcgcga ctcgccaccc
    11221 acagcggcga agccgcgcgc atggaataag aggagcgcgc catggaagtc atgattgcgg
    11281 ccgtactgat cggcctgatg ctggcgctgc tcgccggcgg cgtatggatc ggcctcgcgc
    11341 tgatggcgat cggcatcctc ggcatgctcg gcttcacgcc gcgcgcgccg ggcgacggca
    11401 tggcggtggc gatctggagc cacggttcga gctggaccct gaccgcgctg ccgctgttcc
    11461 tgtggatggg cgagatcctc ttccgcacca agctgtcgga agacatgttc aagggcctgt
    11521 cgccctggct ggaacgcctg ccgggccgcc tgctccacac caacatcatc ggctgcacct
    11581 tgttcgccgc ggtgtcgggc tcgtcggcgg cgacctgcgc caccatcggc aagatcgcgc
    11641 tgcccgagct gcagcgccgc ggctaccccg agtcgatggc gctgggcacc ctggccggtg
    11701 ccggcacgct gggcctgctg atcccgccct cgatcatcat gatcgtgtac ggcgtggcgg
    11761 cggacgtgtc gatctccaag ctcttcatcg gcggcgtgct gcccggcatc ctgctggcga
    11821 gcctgttcat gggctgggtg gtggtgtggt cgctgatgaa cccgggcaag atccccgccg
    11881 ccgacctcaa gaccgacttc atgggcaagc tgcgcgcctc gaagaacctg atcccggtga
    11941 tcctgctgat cggcggcgtg ctcggctcga tctacagcgg catcgccacc gcgaccgagg
    12001 ccgccgcgat cggcgtgatc ggcagcctga tcatcgccgc cagccagcgc acgctcaatc
    12061 gcaagagctt cttcggtgcg ctgatgggcg ccacccggct gtactgcatg atcgcgctga
    12121 tcctcgccgg ctcggccttc ctgaccctcg cgatgggcta catcggcctg ccccgccatc
    12181 tcgccgagtg gatcggcggg ctcggcctca ccccgggcat gctgctggtg gcgctggcga
    12241 tcttctacat cctgctcggc tgcttcctcg acggcatctc gatcgtggtg ctgacgatgg
    12301 cggtgctgat gccgaccatc caggccgcgg gcatcgatcc gctgtggttc ggcatcttcg
    12361 tggtggtggt ggtggagatg gcgcaggtca ccccgccggt cggcttcaac ctcttcgtgc
    12421 tgcagggcct caccgggcgc gacatgacgg tgatcgcgcg ctacgcgctg ccctacttcc
    12481 tgctgatggt gctggcggtg gtgctgctgt acaccttccc cggcctggtg acctggctgc
    12541 ccgggcacat gatcgggtga cgggctccgc gcccgcggcc gctggcgttc agtcgccggg
    12601 cgcgggcgcc gtatctgcgc aagggccggc ggcgagttcc gccgcacgcg cctcggccgc
    12661 cagccagtcg tgcaccgtgg ccacgcgcgg atcggcgagc gccccggcgg aatggatcac
    12721 gcagtagcgc ttgccggtgc gcaccgactg cgcgaagggc gccaccaggc ggccgctgtc
    12781 gagctcgtcg gtgatcaggg tgcggcgcgc gatcgccacc cccatgccgg cgatcgcgca
    12841 ctgcatggtg atgtggttgc ggttgaacat gtagccgcgc cgtacctcca cctggggtgc
    12901 gccgatggcc ttcaggtagc gctcccactc ggcgtagggc gggctcccgc gccaggcggt
    12961 catgtcgtgc agcagcgggt agtggcgcag gtcctcggcg ccgagcagcg gtggacggcc
    13021 ttcgagcagg cgtggcgagc acaccgggaa gatctcctct tccagcagca cggtgctctg
    13081 gaagccgggg tagctgccgt cgttgaggtc gatcgcgagg tcgaactcgc cgtcgcgtag
    13141 cgagtacgtg ctgtcctcgg cctcgacctg cagctcgatc tgcggaaagc gcgccatcag
    13201 gcgtggcaga cgcggcacca gccaggtggc gaggaaggag ggcagggtgc gcacccgtac
    13261 cacgccgctg agcgcgccgc cacgcaggcg gccgatggcg agctcgaccg cggcgtaggc
    13321 cggatcgacc gcggcgagca ggcgcttgcc cgcgtcggtg agctcgagcc ggcgcggcag
    13381 gcggcggaac aggcgctggg cgagccagtc ctcgagcagc ttgatctgct ggctcaccgc
    13441 gccggtggtg atgctgagct cctcgccggc gcgggtgaag gacaggtgac gcgcgacggc
    13501 gaggaagacg cgtagccggg tatggatctg ggagggtgcg ctcgtagcca tggtttagtt
    13561 ttgctaaaga aaagatcaat aactatcgat tgagcacccg cccatccagc tggagaatgc
    13621 cgctcatgca ctgataagta ttttcgtgct tagcagaatt aaatcaacaa gccgtttccc
    13681 ctcggagtct cccatgtcca tcagcgtact cgacctgttc aagatcggcg tcggtccgtc
    13741 cagctcgcac acggtcgggc cgatgcgggc cgcgcacgat ttcgtcgccg agctgcagcg
    13801 ccgcggcctg ctcgaccagg tgcgccgcat cgaggcgaag ctctacggct cgctgtcggc
    13861 gaccggcgtt ggccacggca ccgaccgcgc ggtggtggcc ggcctgatgg gcgcgcgccc
    13921 ggacgaggtt gatcccgact tcctggtggg cgcggtcgat gcggtgcgca tcgatgccag
    13981 cctgccgcta ggcggcacga cggcggtgcc cttcgagtgg acgcgcgacc tgcgcctgct
    14041 gccggtgagc ctgccctacc atcccaacgc gatgcgcctg gccgcgcacg gcgaggctgg
    14101 cgtgctgtac gagaacacct actactcggt gggtggcggc ttcgtggtcg acgaggcgca
    14161 ggcgcagtcg ggcgcgccgg ccgaggccgc ggtcgccgtg ccctatccct tcgacaccgg
    14221 cgcagagctg ctcgcccagt gccgccgcca cggcctgcgc atcagcgagc tgatgctggc
    14281 caacgagtcg gcctggcgca gcgaggcgga gacgcgcgcc gcgctgctgc gcatctggga
    14341 cgtgatgcgc gcctgcgtga cgcgcggcgt gggccacgag ggcgtgctgc ccggcgggct
    14401 caacgtgcgc cggcgcgcgc cggggctgta tcgcaagctc agcgagcagg cgagcgggcg
    14461 caacatgatc agcgactcga tgagcgcgct cgactgggtc aacctctacg cgctcgcggt
    14521 caatgaagag aacgccggcg gcggccgcgt cgtcacggcg cccaccaatg gtgcggccgg
    14581 tatcatcccg gcggtgatgc actactacat gaagttcatg cccggcagcg acgagcgcga
    14641 cgtggagaac ttcctcctcg ccgccgccgc ggtcggcgtg ctgtgcaaga agaacgcctc
    14701 catctccggc gccgaggtcg gctgccaggg cgaggtcggc tcggcctgcg cgatggccgc
    14761 cgcgggcctg gccgaagtgc tcggcggcac gcccgagcag gtcgagaacg ccgccgagat
    14821 cggcctcgag cacaacctcg gcctgacctg cgaccccgtc ggcggcctgg tgcaggtgcc
    14881 ctgcatcgag cgcaacgcga tggcctcgat caaggccatc aacgccgccc agctcgccct
    14941 gcgcggcgac ggcgcgcaca cggtctcgct cgaccaggtg atccggacca tgcgcgacac
    15001 gggccgcgac atgctcgaca agtacaagga gacctcccgc ggcggcctcg ccgtcagctt
    15061 tccggagtgc tgagcgccgc cacaccggat ctccagccga atgtccaccc gttccacccc
    15121 cgacccggca cagcccatcc cgcacgtgcc cgggtccagc ctcacccggc ttccctaacc
    15181 acaacgtcat ccacgaggag acactcaatg accaccgcaa accccacggc tctgcagcgc
    15241 gtgatgcgca tgagcctcat ctcccagatc gccatcggtc tggtcctcgg catcgcactg
    15301 gcgctgatcg cgccgggcgc caccaagtcg gtcgcgctgc tcggcgacct cttcatcgcc
    15361 gcgctgaagg cggtcgcgcc gatcctggtc ttcgtgctgg tggcggcggc gatcgccaac
    15421 cacaggcacg gccagcccac gcacatgcgc ccgatcctcg tcctctacct gctcggcacc
    15481 ttcgccgccg cggtggtggc ggtgatcgcc agcttcgcct tcccgaccac gctggtgctc
    15541 gacaccgcgg ccgcgaccgg caacccgccg ggcggcatcc tcggggtgct caagaacctg
    15601 ctgctgagcg tcgtctccaa tccggtgcgc gcgctgatgg aagccaattt catcggcatc
    15661 ctggcgtggg cgatcggcct cggcgtcgcg ctgcgccatg ccgcggagag cacccgcaag
    15721 atggtcaatg acgtcgcgga tgcggtgtcc tcgatcgtgc aggtggtgat ccgcttcgcc
    15781 ccgctcggca tcttcggcct ggtcgccgcc accatgggcg agtccggctt cgacgcgctg
    15841 ctcggctacg gcaagctgct gatggtgatc gtgggctgca tggtcttcgt cgccctggtg
    15901 gtcaacccgc tgttcgtgtt ctggaagctg cgcaccaacc cctatccgct ggtgttcacc
    15961 tgcctgcgcg agagcggcgt caccgccttc ttcacccgca gctcggccgc caacatcccc
    16021 atcaacctcg agctgtgcaa gcgcatgggc ctgcacgagg acacctattc gatctcgatc
    16081 ccgctcggcg ccacgatcaa catggctggc gcggcgatca cgatctcggt gatggcgctc
    16141 gccgcggcgc acaccctcgg catcgcgacc gacttcccga ccgcgctgct gctgtgcctg
    16201 gtgtccgcgc tcgccgcatg cggcgcctcg ggcgtggcgg ggggctcgct gctgctgatc
    16261 ccgatggcca ccagcctgct gggcgtccag gccgacgtgg cgatgcaggt ggtggcgatc
    16321 ggcttcgtca tcagcgtggt gcaggactcg tgcgagaccg cgctgaactc ctcgaccgac
    16381 gtcctcttca ccgccgccgc ctgcgcggcg gagagcggcg agccgctcca gctggatcgc
    16441 acctgatccc ggcatgaccg tgcgcctgcg ccacggcgca ggtgtccgtc cggcggccgt
    16501 cgcggcctcc gggcgtggcg gggctccggc cccgcgcctt ccctgaatca agccctgccc
    16561 ggggggcttc caatccccgg gcactgcaac ggagtcgtca tgaacaagaa tcccgcaact
    16621 gccgtcgaac agagcggcaa gccccaatcc ccggcgcgcg agcgcttcct cgcccatctg
    16681 cacggtgtcg tcgatcagat ccgcgccgac ggcttctaca aggccgagcg cgtcatcgac
    16741 agccctcagg gtgcccaggt ccacctcggc accggtgcgg acgtcctcaa cttctgcgcc
    16801 aacaactacc tcgggctcgc caacgacccg cgcctggtca aggccgccca ggacggcctg
    16861 gccgaggacg gcttcggcat ggcgtcggtg cgcttcatct gcggcacgca gtcggtgcac
    16921 aagcagctcg aggcgagcat ctcgaagttt ctcggcaccg aggacaccat cctgtacggc
    16981 agctgcttcg acgccaacgg cggcctgttc gagaccctgc tcaccgaaga ggacgcggtc
    17041 atctccgacg agctcaacca cgcctccatc atcgacggcg tgcgcctgtg caaggccagg
    17101 cgctaccgct accgcaacaa cgacatggcc gacctcgagg cgcagctgca ggccgcggac
    17161 gccgccggcg tgcgcttcaa gctgatcgcc accgacggcg tgttctccat ggacggcgtg
    17221 atcgccgacc tgaagtcggt ctgcgacctg gccgacaaat atggcgcgct ggtgatggtc
    17281 gacgactccc atgccgtcgg cttcgtcggc acgcacggcc gtggaacggc ggaatactgc
    17341 ggcgtggaag gccgcgtgga catcatcacc ggcacgctcg gcaaggcgct ggggggctcc
    17401 tcgggaggct atacctcggg gcacaaggag atcatcgaac tgctgcgcca gcgctcgcgg
    17461 ccctatctgt tctccaacac gctcgcgccc tccatcgccg cggcctcgat caaggtgatg
    17521 gagctgctcg cgagtgacga aggtcttgaa ctgtgccgtc gcgtcaagga aaacggcgaa
    17581 catttccgcc gcgagatgac caagctcggc ttcacgctgg cgggcaagga tcaccccatc
    17641 gtcccggtca tgctcggcga tgcgcagctg gccggtcgca tggccgaaga gctgctgaag
    17701 gaaggcatct acgtcatcgg cttctccttc ccggtcgtgc ccaagggcaa ggcccgcatc
    17761 cgcacgcaga tgagcgccgc gcacaccacg gcgcagatcg accgcgcggt ggcggccttt
    17821 gccaaggtgg gcaaggcact cggcgcgatt gcgtgagcac gaccactgat atcgattgag
    17881 aggaatccca tgaaggcctt ggccaaattg accccgtcgg tggggctcac gctgaccagc
    17941 gtggaaaagc cggaagtcgg acccaacgac gtcctcatca agatcaagaa gaccgcgatc
    18001 tgcggcacgg acatccatat ctggaagtgg gacgagtggg cgcagaagac cattcccgtc
    18061 cccatgcacg tcggccacga gtacgtcggc gtgatcgccg agatgggcca ggaggtgcgc
    18121 ggcttcaaga tcggcgaccg cgtgtcgggc gaggggcaca tcacctgcgg tttctgccgc
    18181 aactgccgcg ccggccgtcg ccacctctgc cgcaacaccg tgggcgtcgg cgtgaaccgc
    18241 gcgggcgcgt tcgccgagta cctggcgatc ccggcggtca atgcgttcaa gatccccgac
    18301 gacatctccg acgacctcgc ggcgatcttc gaccccttcg gcaacgccac ccataccgcg
    18361 ctgtccttca acctggtcgg cgaggacgtg ctcatcaccg gcgccggccc gatcggcatc
    18421 atggcggtgg cgatcgcgcg tcacgtcggc gcgcgccatg tcgtcatcac ggacgtcaac
    18481 gatcaccgcc tggagctcgc ccgcaagatg ggcgcgacgc gcgccgtgaa tgtgagccgc
    18541 gagaacctcg ccgacgtgaa gaccgagttg cacatggtcg aaggcttcga cgtcggcctc
    18601 gagatgtcgg gcgtgccgtc ggcgttcacc tcgatgctcg agcacatgaa ccacggcggc
    18661 aagatcgcgc tgctgggcat tccgccgtcc aacaccgcga tcgactggaa ccaggtcatc
    18721 ttcaagggcc tggagatcaa gggcatctac ggccgcgaga tgttcgagac ctggtacaag
    18781 atggtcgcca tgctgcagag cgggctggac ctgagcccga tcatcaccca ccactacccg
    18841 gtcgaggagt tcaagaccgg cttcgagacc atgctctcgg gcaacagcgg caaggtgatc
    18901 ctggactggg aagcgctggc cgcctgatcc ttgtgctgtg gcaaacagga cagagcccgc
    18961 gcaggcccgg gctctgtccg ttggcgggcc cgctcagagt tcgtccagcc gcgccagcca
    19021 gtgcgcggcc tgcgccagcg tggcggcgcg ctcgcccgcg tccatcttcg cccacgcctt
    19081 gtacggcatc gccatgcgcg ggttgcgtgc gaagcgcgcc tcgtggcgca gcaggaaatc
    19141 ccagtacagg ctgttgaagg ggcaggccgg cttgccgctg gcgccgtggc ggcgcctggg
    19201 gtcgtaggcg cagcccttgc aatagtc
//
LOCUS       DAOQBS010000053        19183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_1999_length_19183_cov_14.822146, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000053 DAOQBS010000000
VERSION     DAOQBS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 19183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19183
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1999_length_19183_cov_14.822146"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKN25_07770"
     CDS             <1..380
                     /locus_tag="PKN25_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HNV90833.1"
                     /translation="GKLLSYNCSPSFNWKKNLDDKTIAKFQDELSALGYKYQFITLAG
                     IHINWFNTFKFAHAYARGEGMKHYTEMVQEPEFAAREQGYTFVSHQQEVGAGYFDDVT
                     TVIQGGSSSVKALTGSTEEEQFH"
     gene            complement(638..1177)
                     /locus_tag="PKN25_07775"
     CDS             complement(638..1177)
                     /locus_tag="PKN25_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNV90834.1"
                     /translation="MKNWIKTSIAAAIIATGAIGSTAALAWGGHCDGPRGGKAGWSQM
                     APEQMKAKMAARAELHMARLELALALAPEQKPAFERFKADMKSRAERMGSMMAERRAA
                     EPPKTAIERMQRMEEMSKLRQAEMGEARKSVETFYATLSDAQKTVFDAEFEKMGRKGE
                     RGMKGGGPRDGGSMGPGRG"
     gene            1374..2144
                     /locus_tag="PKN25_07780"
     CDS             1374..2144
                     /locus_tag="PKN25_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV90835.1"
                     /translation="MNTSDHILIVDDDRDIRQLLADYLEKQGLRCTTAADGREMKAAL
                     ETHRIDLIVLDLMMPGEDGLTLCRNLRASGGPHANTPILMLTARGEDMDRIIGLEMGA
                     DDYLAKPFVPRELFARIRAVLRRTRALPPNLDAAPPANARLLRFAHWRLDTINRHLVD
                     AEGTVVALSGAEYRMLAVFLSHPQKVLSRDQLMELTQGREADVFDRSIDLLVSRLRQR
                     LGDNAREPAIIKTVRNEGYVLAAEVVAETADTDGSGAR"
     gene            2141..3457
                     /locus_tag="PKN25_07785"
     CDS             2141..3457
                     /locus_tag="PKN25_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV90836.1"
                     /translation="MSLPLPRSLFARLMLIWLVGMTLVLAVSLALFLGERERIGRSAL
                     FEGVAQEIASVAELLDRMSPPERERWIDEIGRRRLRLALRPPHDHLRPLADDHPLQTA
                     LREAMPQRPATLYTHPRDDRPHPGLAIVLALADGTPLQVRLPGIPPLPPIMPHQRGPF
                     LAALAALVLGVGLLTWIAVRIATRPLSRMAEAARALGEDPTRAPMDTRGPTEVAQAAA
                     AFNQMQQRVAEHVAERTRILAAISHDLQTPITRLRLRAEMVDDEGLRARIQSDLDAMQ
                     GLVKEGLAYARSLDERSPAQAIELGPLLQALAEDARDMGWTVRVPEGAQGRIVGNPSA
                     LRRALWNLIENAVKFGGKVEVALACVGEHCEIRVRDHGPGLAEDELEKVFEPFYRTEA
                     SRNRETGGTGLGLAITRNLLHAQGGEVTLRNHPDGGLEAVVRLRVP"
     gene            complement(3409..4971)
                     /locus_tag="PKN25_07790"
     CDS             complement(3409..4971)
                     /locus_tag="PKN25_07790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNV90837.1"
                     /translation="MESVHPHARRRLLARLADARAVDLVVVGGGATGLGCALDAAARG
                     HSVLLVEARDFAAGTSSRSTKLIHGGVRYLAQGRIGLVREALRERGRLLRNAPALVHP
                     LRFVVPARNLAERLLLRLGLGAYEALAGRDGLGGCTLLDAAGLHAALPGLREEGLGGG
                     VAFRDAQFDDAGLALALARTASAHGALLLNHCAAHELVVEDGRLRGVVVREAEGGQAH
                     HVAARAVINATGVWGDTLRRRADPAARALLRPSQGVHLVVDADCLPGSDALLVPRSPD
                     GRVLFMIPWCGKVLIGTTDTPRADTPAEPEPLAGEVDELLAAAARWLRRPPAREAVRS
                     VFVGLRPLLGEDGGDGASSRLSREHRVEVSAQGLVSVLGGKWTTYRAMAEQAVDAAEV
                     VGGLARRASPTASLALDCGVCAGEAGEADLPGAPPGLPPGVLPGAAAVRRALREDFAL
                     SVEDVLARRSRALFLDAAAAAAVAPAVADLLAAELGWSAQRRAAELATFRALAARYGT
                     RSRTTASSPPSG"
     gene            complement(4994..6487)
                     /gene="glpK"
                     /locus_tag="PKN25_07795"
     CDS             complement(4994..6487)
                     /gene="glpK"
                     /locus_tag="PKN25_07795"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNV90838.1"
                     /translation="MAYLLALDQGTTSSRAIVFDADGRVRGLAQQAFAQHFPAPDRVE
                     HDPQDILRTQLDCAREALANAGVAAREVAAIGIANQRETTVLWERASGRALAPAIVWQ
                     DRRTAVECERLRAAGCAEDVRARTGLELDPYFSATKLAWLLDHVPDARVRAERGELAF
                     GTIDSWLLWHLSGGRLHLTDAGNAARTLLFNLHTLDWDPVLLERFRIPPVLLPRVVAS
                     SGVCGETDPALFGAAIPIAGIGGDQQAATFGQACLAPGMAKNTYGTGCFLLMNTGHVP
                     VASAHRLLTTVGWTGVDAAPSYALEGSVFMGGAVVQWLRDGLGLVKRASEVEALAASV
                     PDSGGVVLVPAFTGLGAPYWDPHARGTLLGLSRGSGAAHIARAALEAIAMQSADLVQA
                     MARDGAAPLAELRVDGGAAANDLLMQMQADLLGVPVLRPRLLETTAFGAACLAALGCG
                     LWKRPQEIATHWQLDRRFEPRWPAARRDEARAVWVRAVERARAWAQD"
     gene            6611..7483
                     /locus_tag="PKN25_07800"
     CDS             6611..7483
                     /locus_tag="PKN25_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV90839.1"
                     /translation="MPAFWMIVSCFLFACMGVCVKLASGAFSTGEIVFWRGLVSMLMM
                     VGLAASRGIGLKTPHWKMHLSRSVSGSIALGCYFFAIGLLPLATAVTLNYTSPLFVAL
                     VLALFFGERLRAIAVLAILAGFAGVVLLLRPTLQPEQWLGALAGLGSGALASLAYVSL
                     RELGRAGEPEVRTVFWFSVMTSTMGLAWALAGELHRPDAGQLATLLGVGLFGGLAQLA
                     MTRSYRYGRTVVSANLSYATVIFASVFGMLLWDELMPASSWLAVGLIVAGAIGVSLAT
                     PKPAPLPARAVATD"
     gene            7543..7920
                     /locus_tag="PKN25_07805"
     CDS             7543..7920
                     /locus_tag="PKN25_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNV90840.1"
                     /translation="MISTESTDKLVSLVVFGEFTLADYKEFEDVVNYRVQFSGPVDLL
                     FDLRQMAGFTLDVAWEEIKFSRQHAGDFRRIAVLTEDQWLTWSAWVSQLFVTAEVKVF
                     DDEDEARAWLAAETTAETTGSAQ"
     gene            7917..8771
                     /locus_tag="PKN25_07810"
     CDS             7917..8771
                     /locus_tag="PKN25_07810"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNV90841.1"
                     /translation="MNASYATLIGAIDLAQHLRDADWVVFDCRADLVDPVFGQRAYDV
                     AHVPGAFFIDLETGLSGPKTGRNGRHPLPDPASLAARLAACGVGNHSQVVAYDDAGGM
                     FAARLWWMLRWLGHHRVAVLDGGLQAWMAAGLALDDEPPRARPATYTLALRPDRVDTQ
                     YVQQHLGRPDMLLLDARSPDRFRGENEVLDPVGGHIPGARNRFFRDNLDARGCFKQAS
                     VLREEFASLLGAYTPRQVVHQCGSGVTACVNLLAMEAAGLTGSRLYAGSWSEWCADPT
                     RPMARGPA"
     gene            complement(8826..9632)
                     /gene="murI"
                     /locus_tag="PKN25_07815"
     CDS             complement(8826..9632)
                     /gene="murI"
                     /locus_tag="PKN25_07815"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNV90842.1"
                     /translation="MSAAARPIGVFDSGVGGLTVVRALMERLPLEDIVYFGDTARVPY
                     GVKSVATIEHFTAQITEFLLQRDVKMLIIACNTMAAVAADVVHRLAAGIPVLDVIEAG
                     ARAAVAASPGRRIGVIGTPTTINSNAYARRMHELDPAVRVYSQACPLFVPLVEEGWLD
                     HEVTRLTAQEYLRPVLAEEVDSLVLGCTHYPLLKPLLRDVAGARVRLIDSALTTAELA
                     AERLQLSGLARGGGGVADYRFVVSDVPLRFQTIGERFLGRSLGAVEKVDW"
     gene            complement(9664..10863)
                     /gene="tyrS"
                     /locus_tag="PKN25_07820"
     CDS             complement(9664..10863)
                     /gene="tyrS"
                     /locus_tag="PKN25_07820"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNV90843.1"
                     /translation="MSDVQAAIDLIKRGADAVLIEAELVEKLKRGKPLRVKAGFDPTA
                     PDLHLGHTVLINKMRHFQELGHQVQFLIGDFTGMIGDPSGKNSTRPPLSREQILENAR
                     TYQEQVFKILDPEKTEICFNSAWMNDLGAAGMIRLAAQQTVARMLERDDFAKRYGNNQ
                     PIAIHEFLYPLCQGYDSVAMKADVELGGTDQRFNLLMGRELQKHYGQEPQCVVMVPLL
                     EGLDGVNKMSKSLGNYIGIAEPAKEIFGKTMSVSDTLMWRYYELLSFRSSAEIAQLRA
                     DVEGGRNPRDVKVMLAMEFVARFHGQRAAEEALADFEARFQRNAIPDDIPQVNVNVGA
                     GGLPLFQVLKQAGLTGTTSEAMRMVEQGGVRIDGERAEDKGLLLRAGETVVLQVGKRK
                     FASVVLS"
     gene            11048..12277
                     /locus_tag="PKN25_07825"
     CDS             11048..12277
                     /locus_tag="PKN25_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNV90844.1"
                     /translation="MLGVVAATAVAPGSLPSDLLVERVVEALPAPAVTRTGTDASLPF
                     VHYERIQAGDTLQAMFNRLRIDDAEALAHLSASDAGRKALRQLRAGRSVTAVVSPQGR
                     LLSFSLPVGNTDAEVVLERDDAGLALRESSGATQTTMVEMRSGTITHSLFGATDDAGL
                     PDNVATQLATIFGTWIDFHTDLRKGDRFNVVYEAIYEEGNPVRAGRILAAEFINNGDR
                     HAVVLYRGPSGKEQYYSDDGESLQQGFLRSPLEFSRVSSNFGRRLHPIHGNWRDHNGT
                     DFSAPVGTPVMATSDATIEFIGTQRGFGNLIVLKHRSDITTHYAHLNGFAKGLAKGQA
                     VSQGDLIGYVGCTGWCTGPHLHYEVRLKDVPADPMTVALPMADSFNDRELAAFKRNTA
                     HLRQRFALLNYELADAR"
     gene            complement(12345..12695)
                     /gene="erpA"
                     /locus_tag="PKN25_07830"
     CDS             complement(12345..12695)
                     /gene="erpA"
                     /locus_tag="PKN25_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNV90845.1"
                     /translation="MSAEVATPEIMVFTDSAANKVRELIEEEGNPDLKLRVFVSGGGC
                     SGFQYGFTFDEEVNEDDTTFEKNGVLLLVDPMSYQYLVGAEIDYTEGLEGSQFVIRNP
                     NATSTCGCGSSFTV"
     gene            complement(12786..13823)
                     /gene="argC"
                     /locus_tag="PKN25_07835"
     CDS             complement(12786..13823)
                     /gene="argC"
                     /locus_tag="PKN25_07835"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNV90846.1"
                     /translation="MVKVGIVGGTGYTGVELLRLLARHPGVELTAITSRGEAGMAVSD
                     MFPSLRRRVDLKFVTPQDAALEKCDAVFFATPNGIAMKQAAELVAAGVRVIDLAADFR
                     IRDVAEWQKWYGMEHAAPELVAEAVYGLPEVNRAQIRGARVLANPGCYPTAVQLGFLP
                     LVEAGLIDTDHLIADAKSGVSGAGRKAEVHTLLPEAADSFKAYGVPGHRHLPEIRQGL
                     ALAAGHAVGLTFVPHLTPMIRGIHATLYGRLKPGVDAADLQGLYEKRYAGEAFVDVMP
                     AGSHPETRSVRASNLCRIAVHRPQGGDTVVVLSVIDNLVKGAAGQAVQNLNIMFGFDE
                     ETGLDIVPVSP"
     gene            complement(14002..14394)
                     /gene="rpsI"
                     /locus_tag="PKN25_07840"
     CDS             complement(14002..14394)
                     /gene="rpsI"
                     /locus_tag="PKN25_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNV90847.1"
                     /translation="MAVTYNYGTGRRKTAVARVFIKPGTGNIVVNGKPVDEFFSRETG
                     RMIVRQPLVLTGNEGRFDIMVNVVGGGESGQAGAVRHGITRALVDYDAGLKGDLRAAG
                     FVTRDAREVERKKVGLRKARRAKQFSKR"
     gene            complement(14406..14834)
                     /gene="rplM"
                     /locus_tag="PKN25_07845"
     CDS             complement(14406..14834)
                     /gene="rplM"
                     /locus_tag="PKN25_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNV90848.1"
                     /translation="MKTFSAKPHEVKRDWFVVDGTDKVLGRLAAEVARRLRGKHKAIY
                     TPHVDTGDFIVVVNVEKLRVTGNKAQDKKYYRHSGYPGGIYETNFTKLQQRFPERVLE
                     KAVKGMLPKGPLGYAMLKKLKCYAGPSHPHTAQQPQVLEI"
     gene            15463..15882
                     /locus_tag="PKN25_07850"
     CDS             15463..15882
                     /locus_tag="PKN25_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNV90849.1"
                     /translation="MECTVKWLDGMSFIAETGTGHLVAMDGAPDAGGRNLAPRPMELL
                     LAGAGGCTAFDVMLILKRGRHDVRGCSVRLEAERAESDPKVFTRIRFIYTITGRKLKR
                     ETVERAVHLSAEKYCSASIMLGKTAEMIHEVELVEAD"
     gene            complement(15938..16558)
                     /gene="coq7"
                     /locus_tag="PKN25_07855"
     CDS             complement(15938..16558)
                     /gene="coq7"
                     /locus_tag="PKN25_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HNV90850.1"
                     /translation="MIDQAIIEFDKALRTVFASARSVRAMPGEGVPDAELGAAERSHA
                     AALMRVNHVGEVCAQALYQGQSIMSREPAIRDTLRQAAQEETEHLAWTERRIAELGGR
                     KSLLNPLWYGGALALGLAAGRFGERWNLGFLAETERQVEQHLKSHLDTLPAADRRSRA
                     IVEQMKVDEAEHAATALRLGAHELPAPAKGAMKLAARVMTTLAYRL"
     gene            complement(16555..17112)
                     /locus_tag="PKN25_07860"
     CDS             complement(16555..17112)
                     /locus_tag="PKN25_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HNV90851.1"
                     /translation="MSPFPIRFLRPSLGALPRAGVVALALLSAPASAGFLSDADPDWK
                     EGEATMPAAPRAAALRAIDVGTPSPNAFFVDEDSVGVGADGVVRYTLVVRTRGGAENV
                     SFEGIRCATFERRIYASGRKDGTWAALKNSAWQPIVESGYNRPRAALAHDYFCDGPAG
                     PRDREHALRLLRERRDPIDPSGVHQ"
     gene            complement(17266..17859)
                     /locus_tag="PKN25_07865"
     CDS             complement(17266..17859)
                     /locus_tag="PKN25_07865"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNV90852.1"
                     /translation="MLDREGFRPNVGIILVNARNEVFWGKRIREHSWQFPQGGIKHGE
                     SPEQAMYRELHEEVGLRPEHVRILGRTRGWLRYEVPKHWIRREWRNTYRGQKQIWFLL
                     RLVGRDSDVCLRASTHPEFDAWRWSNYWVPLEAVIEFKRQVYQLALIELARILFRSRS
                     VELPDSYRLPEPEPAPEPVRVVLGERAEHGLRDAMRK"
     gene            18027..>19183
                     /gene="proS"
                     /locus_tag="PKN25_07870"
     CDS             18027..>19183
                     /gene="proS"
                     /locus_tag="PKN25_07870"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNV90853.1"
                     /translation="MRASQFHLFTLKEAPSDAEVVSQKLMLRAGMIRKVAAGIYSYMP
                     MGLRAIRKVEAIVREEMDRAGAMELIMPMVQPAELWDETGRWDKMGDELLRFKDRHER
                     DFALQPTSEEVVTDIARQELKSYRQLPKNFYQIQTKFRDERRPRFGVMRGREFTMKDA
                     YSFDRSEEAAGRSYDIMFAAYKRIFDRLGLEYRAVAADTGAIGGDRSHEFQVIADTGE
                     DAIVYCPDSDYAANIELAEAVSLLARRAEPARALAKTPTPGKATCEDVAALLGVPLAT
                     TVKSLVLATDDVDEKGKPAGVTVWLLLVRGDHALNEVKAGKLEGLKAGFRFATEAEIL
                     EHFGCKPGYLGPIGLKKPVKVIADRTVAHMADFICGANEADFHFTGVNWGR"
BASE COUNT         3044 a         6935 c         6352 g         2852 t
ORIGIN      
        1 ccggcaagct gctgtcctac aactgctcgc cgtccttcaa ctggaagaag aacctggacg
       61 acaagaccat cgccaagttc caggacgaac tctcggcgct gggctacaag taccagttca
      121 tcaccctggc cggcatccac atcaactggt tcaacacctt caagttcgcc cacgcctacg
      181 cccgcggcga aggcatgaag cactacaccg agatggtgca ggaaccggaa ttcgccgctc
      241 gcgagcaggg ctacaccttc gtgtcgcacc agcaggaagt cggcgccggc tacttcgacg
      301 acgtcaccac cgtgatccag ggtggctcgt cctcggtcaa ggcgctgacg ggctcgaccg
      361 aagaagagca gttccactaa gagactgctt ccctcataac gggggaggag acggaaggcc
      421 accctgcggg gtggcctttt tcgtttttcg acgatgaccc gcaaaccgat ctggcgtcat
      481 cgcacccgca gttcccgcag agccacgcag gatccgggcg ggactgcagt gtgcgggacc
      541 gcccgatcgc gacttgcgcc gcgcctgccc gaatacaccg gggccctccc gaaggacggc
      601 cccggacctc gagacaagcc gcggatgcgg cgaggcgtca gccgcggccc ggccccatgc
      661 tgccaccgtc gcgcggaccg ccgcccttca tgccgcgctc gcccttgcgg cccatcttct
      721 cgaactccgc atcgaacacc gtcttctgcg catcgctcag cgtcgcatag aaggtctcga
      781 ccgacttgcg cgcctcgccc atctcggcct ggcgcagctt gctcatctcc tccatgcgct
      841 gcatgcgctc gatcgccgtc ttcggcggct cggcggcgcg gcgctcggcc atcatgctgc
      901 ccatgcgctc ggcgcgcgac ttcatgtcgg ccttgaagcg ctcgaaggcg ggcttctgct
      961 cgggcgccag ggccagcgcc agttcgaggc gtgccatgtg cagctcggcg cgcgcggcca
     1021 tcttcgcctt catctgctcg ggcgccatct gcgaccagcc ggccttgccg ccgcgcggac
     1081 cgtcgcaatg gccgccccag gccagcgctg cggtgctgcc gatggcgccg gtggcgatga
     1141 tggcggcggc gatcgaggtc ttgatccagt ttttcatgat ggctctcctg tgggttgctt
     1201 gtccgtgttg cccgtccgtt cgggcgacgg agccatttga acaccgcgat gtaagccgga
     1261 tttgtccgcg cggcacgcgt ttgcagcctg atgtctcggc agcgcccgca gatacgctgg
     1321 gatacaaagc cggacccacc ccgccttatg atccaggcca tggagaacac cgcatgaata
     1381 cgtccgacca catcctcatc gtcgacgacg accgtgacat ccgccagctg cttgccgact
     1441 acctcgagaa gcagggactg cgctgcacca ccgcggccga cgggcgcgag atgaaggcag
     1501 cgctcgagac ccaccgcatc gacctcatcg tgctcgacct gatgatgccc ggcgaggatg
     1561 gactcaccct gtgccgcaac ctgcgcgcga gcggcgggcc gcacgccaac accccgatcc
     1621 tgatgctcac cgcgcgcggc gaggacatgg accgcatcat cggcctcgag atgggtgccg
     1681 acgactacct cgccaagccc ttcgtgccgc gcgagctctt cgcccgcatc cgcgcggtgc
     1741 tgcgccgcac acgcgcgctg ccgcccaacc tcgacgccgc cccgcccgcc aacgcgcgcc
     1801 tgctgcgctt cgcgcactgg cgcctggaca cgatcaaccg ccacctggtc gacgccgagg
     1861 gcacggtggt cgcgctctcc ggcgccgagt accgcatgct cgcggtcttc ctgtcgcacc
     1921 cgcagaaggt gctgtcgcgc gaccagctca tggaactcac ccagggacgc gaggccgacg
     1981 tgttcgatcg ctcgatcgac ctgctggtga gccggctgcg ccagcgcctc ggcgacaacg
     2041 cgcgcgaacc ggcgatcatc aagaccgtgc gcaacgaggg ctacgtgctc gccgccgagg
     2101 tcgtcgccga gaccgcggac accgacggga gcggcgcgcg atgagcctgc cgctgccgcg
     2161 cagcctgttc gcccgcctga tgctgatctg gctggtcggc atgacactcg tgctggcggt
     2221 gagcctcgcg ctcttcctcg gcgagcgcga gcgcatcggg cgcagtgcgc tgttcgaggg
     2281 cgtggcgcag gagatcgcca gcgtcgccga gctgctcgac cgcatgtcgc cgcccgagcg
     2341 cgagcgctgg atcgacgaga tcggccgccg ccgcctgcgc ctcgcgctgc gcccaccgca
     2401 cgatcacctg cgtccgctag ccgacgacca cccgctgcag accgcgttgc gcgaggccat
     2461 gccgcaacgc ccggccacgc tgtacaccca tccgcgcgac gaccgccccc accccggcct
     2521 ggcgatcgtg ctcgcgctcg ccgacggcac cccgctgcag gtccgcctgc cgggcatccc
     2581 gccgttgccg ccgatcatgc cgcatcagcg cggccccttc ctcgccgccc tcgccgcgct
     2641 ggtgctgggg gtaggcttgc tcacctggat cgcggtgcgc atcgccaccc ggccgctgtc
     2701 gcgcatggcg gaggcggcgc gcgcgctcgg cgaggacccg acgcgcgcac ccatggacac
     2761 gcgcggcccc accgaggtcg cccaggccgc cgccgccttc aaccagatgc agcagcgtgt
     2821 cgccgagcac gtcgccgagc gtacccgcat cctggcagcg atctcccacg acctgcagac
     2881 gccgatcacc cgcctgcgcc tgcgcgccga aatggtcgac gacgagggcc tgcgcgcgcg
     2941 catccagtcc gacctcgacg ccatgcaggg cctggtgaag gaaggcctcg cctacgcgcg
     3001 cagcctggac gaacgcagtc cggcgcaggc gatcgagctc ggccccctgc tgcaggccct
     3061 cgccgaggat gcgcgcgaca tgggctggac cgtgcgcgtg cccgagggag cgcagggccg
     3121 catcgtcggc aacccctcgg cgctgcgccg cgcgctgtgg aacctcatcg agaacgcagt
     3181 gaagttcggc ggcaaggtcg aggtggcgct ggcctgcgtg ggcgagcact gcgagatccg
     3241 cgtccgcgac cacggccccg gcctcgcgga ggacgagctc gagaaggtgt tcgagccctt
     3301 ctaccgcacc gaagcctcgc gcaaccgcga gaccggtggg accgggctcg ggctggcgat
     3361 cacgcgcaac ctgctgcatg cccagggcgg cgaggtcacg ctgcgcaatc accccgacgg
     3421 cgggctggag gcggtcgtgc ggctgcgcgt gccgtagcgc gcggcgagcg cgcggaaggt
     3481 cgcgagctcg gccgcgcgcc gctgcgcgct ccagccgagt tccgccgcga ggaggtcggc
     3541 cacagcaggc gccaccgccg cggccgccgc ggcatcgagg aaaagcgcgc ggctgcggcg
     3601 cgcgagcacg tcctcgacgg aaagggcgaa gtcctcgcgc agcgcgcgcc gcaccgccgc
     3661 ggcgccgggc agcacgccgg ggggaagccc cggaggcgcg cccggcaagt ccgcctcgcc
     3721 cgcctccccc gcgcacactc cgcaatcgag cgccagcgag gcggtcggcg atgcccgccg
     3781 cgccagcccg cctaccacct cggccgcatc caccgcctgc tcggccatgg cgcgataggt
     3841 ggtccatttg ccgcccagca cgctgaccag gccctgcgcc gagacctcga cgcggtgctc
     3901 gcgcgagagc cggctgctcg cgccatcgcc tccatcctcg ccgagcaagg gccgcaggcc
     3961 gacgaagacg ctgcgcacgg cctcgcgcgc cggcggccga cgcagccagc gcgcagcggc
     4021 ggccagcagc tcgtccacct cgcccgcgag cggctcgggc tccgccggcg tatcggcgcg
     4081 cggggtgtcg gtcgtgccga tcagcacctt cccgcaccag ggaatcatga acagcacgcg
     4141 tccgtccggg gagcgcggca ccagcagcgc gtcgctgccg ggcagacagt cggcgtcgac
     4201 caccagatgc acgccctggc tcgggcgcag cagcgcgcgg gcggccggat cggcacgtcg
     4261 gcgcagcgtg tcgccccaca ccccggtggc gttgatcacc gcgcgcgcag cgacatggtg
     4321 cgcttgccca ccctcggcct cgcgcaccac cacgccgcgc agccgaccgt cttccaccac
     4381 cagctcgtgc gcggcgcagt ggttgagcag cagcgcaccg tgggcgctcg ccgtgcgcgc
     4441 cagcgcgagc gccaggccgg cgtcgtcgaa ctgggcatcg cggaaggcca cgccgccgcc
     4501 cagcccctcc tcgcgcaatc cgggcagcgc cgcatgcagc ccggccgcgt cgagcagcgt
     4561 gcagccgccc agcccgtccc gacccgcgag cgcctcgtag gcgccgagcc cgagacgcag
     4621 caggagccgc tccgccaggt tgcgcgccgg caccacgaag cgcagcggat gcaccagcgc
     4681 gggcgcgttg cgcagcaggc gcccgcgctc gcgcaaggct tcgcgcacca ggccgatgcg
     4741 gccctgggcg aggtagcgca cgccgccgtg gatcagcttg gtcgagcgcg agctggtgcc
     4801 ggcggcgaag tcgcgcgcct ccaccagcag caccgaatgg ccacgcgccg ccgcgtcgag
     4861 cgcgcagccc agcccggtgg caccgccgcc gaccacgacg aggtcgaccg cgcgcgcgtc
     4921 ggcgaggcgg gcgagaagcc gacgacgggc gtgcggatgg acggattcca tgagcgcgac
     4981 cggcgcgagc cgctcagtcc tgcgcccacg cccgcgcacg ctcgaccgcg cgcacccaca
     5041 cggcgcgtgc ctcgtcacgc cgtgccgccg gccagcgcgg ctcgaagcgg cggtcgagtt
     5101 gccagtgcgt ggcgatctcc tgcggccgct tccacagccc gcagccaagc gcagccaggc
     5161 aggcggcgcc gaaggcggtg gtctcgagca ggcgcggacg cagcaccggc acgccgagca
     5221 ggtcggcctg catctgcatc agcaggtcgt tggccgcggc gccgccatcg acgcgcagct
     5281 cggcgagcgg cgcggcgccg tcgcgtgcca tcgcctggac gaggtcggcg ctctgcatgg
     5341 cgatcgcctc cagtgccgca cgcgcgatgt gcgccgcacc gctgccacgc gacaggccga
     5401 gcagggtgcc gcgcgcgtgc gggtcccagt acggcgcgcc cagcccggtg aaggccggca
     5461 ccagcaccac cccaccgctg tcgggcacgc tcgccgccaa ggcctcgacc tcggacgcgc
     5521 gcttcaccag cccgaggccg tcacgcaacc actgcaccac cgcaccgccc atgaacacgc
     5581 tgccctcgag cgcgtagctg ggcgccgcat ccaccccggt ccaccccacg gtggtcaata
     5641 gccggtgcgc ggacgccacc ggcacatggc cggtgttcat gagcaggaag cagccggtgc
     5701 cgtaggtgtt cttcgccatg cccggggcga ggcaggcctg gccgaaggtc gccgcctgct
     5761 ggtcgccgcc gatgccggcg atcgggatcg cggcaccgaa cagcgccgga tcggtctcgc
     5821 cgcacacccc gctgctcgcc accacgcgcg gcagcagcac gggcgggatg cggaagcgct
     5881 cgagcaacac cggatcccag tccagcgtgt ggaggttgaa gagcagcgtg cgcgcggcgt
     5941 tgcccgcatc ggtcaggtgc agccggccgc cgctcagatg ccacagcagc cagctgtcga
     6001 tggtgccgaa ggcgagctcg ccgcgctcgg cgcgcacgcg cgcatcgggg acgtggtcga
     6061 gcagccaggc gagcttggtg gcggagaaat agggatcgag ctccagcccg gtgcgcgcgc
     6121 gcacgtcctc tgcgcagccc gcggcgcgca ggcgctcgca ctcgaccgcg gtgcggcggt
     6181 cctgccagac gatcgcgggg gcgagggcgc gaccactggc gcgctcccac agcacggtgg
     6241 tctcgcgctg gttggcgatg ccgatcgccg ccacctcgcg tgcggccacg ccggcattgg
     6301 cgagcgcctc gcgagcgcag tcgagctggg tacgcaggat gtcctgggga tcgtgctcga
     6361 cccggtcggg cgcagggaag tgctgcgcga atgcctgctg ggcgaggccg cgcacgcggc
     6421 catcggcgtc gaacacgatc gcgcgcgagc tggtggtgcc ctggtcgagg gcgagcaggt
     6481 aggccatggc gactccgcac gggggctgac cgcgataccg cgagcctagc gccggacgcg
     6541 ccccgccgcc acccgggaaa gccacgatgt gattcaatgg agtccccttc gttgcagcgc
     6601 aacaaccgcc atgcccgcct tctggatgat cgtctcctgc ttcctgttcg cctgcatggg
     6661 cgtgtgcgtg aagctcgcct cgggggcgtt ctcgaccggc gagatcgtgt tctggcgcgg
     6721 gctcgtgagc atgctgatga tggtcggact ggcggcgagc cgcggtatcg gcctcaagac
     6781 gccgcactgg aagatgcacc tgtcgcgcag cgtgtcgggc tcgatcgcgc tcggctgcta
     6841 cttcttcgcg atcggcctgc tgccgctggc gaccgcggtc acgctcaact acacctcgcc
     6901 gctcttcgtc gccctggtgc tggcgctgtt cttcggcgag cgcctgcgcg ccatcgccgt
     6961 gctcgcgatc ctcgccggct tcgccggcgt ggtattgctg ctgcgcccca cgctgcagcc
     7021 cgagcaatgg ctgggcgcgc tcgccggact gggctcgggt gccctggcca gcctcgccta
     7081 cgtgagcctg cgcgagctcg gccgtgccgg cgagccggaa gttcgcacgg tgttctggtt
     7141 ctcggtgatg acctcgacga tgggcctggc gtgggcactc gccggcgagt tgcaccgccc
     7201 cgatgccggg caactcgcca cgctgctcgg cgtcggcctc ttcggtgggc tcgcccagct
     7261 cgcgatgacg cgctcctacc gctacgggcg caccgtggtg tcggccaacc tgagctacgc
     7321 caccgtgatc ttcgccagcg tgttcggcat gctgctgtgg gacgaactca tgccggcctc
     7381 gtcctggctc gcggtcggcc tgatcgtggc cggcgcgatc ggcgtctcgc tcgccacccc
     7441 gaagccggca ccgctgccgg cccgtgccgt cgcgacggat tgagccccgc cggtgcgcgg
     7501 actatagtgg gggcatctcc gatcacctcc agggaggctg ccatgatcag caccgagagc
     7561 accgacaaac tcgtctcgct cgtcgtcttc ggcgaattca ccctcgccga ctacaaggag
     7621 ttcgaagacg tggtcaatta ccgggtgcag ttcagcggac cggtcgacct gctcttcgac
     7681 ctgcgccaga tggcgggctt cacgctcgac gtggcgtggg aggagatcaa gttctcgcgc
     7741 cagcacgcgg gcgatttccg ccgcatcgcg gtactcacgg aagaccagtg gctgacctgg
     7801 agcgcatggg tgtcgcagct cttcgtcacc gccgaggtca aggtcttcga cgacgaggac
     7861 gaggcacgcg cctggctcgc cgccgaaacg accgcagaga ccacgggaag cgcgcagtga
     7921 acgcttccta cgcgacgctg atcggtgcga tcgatctcgc gcagcacctg cgcgacgccg
     7981 actgggtcgt gttcgactgc cgtgccgacc tcgtcgatcc ggtcttcggc cagcgcgcct
     8041 acgacgtcgc gcacgtgccg ggggcctttt tcatcgacct ggagaccggg ttgtcggggc
     8101 cgaagaccgg ccgcaacggc cgtcaccccc tgcccgatcc cgccagcctc gccgcccggc
     8161 tcgccgcctg cggcgtgggc aaccatagcc aggtggtcgc ctacgacgac gccggcggca
     8221 tgttcgccgc gcgcctgtgg tggatgctgc gctggctcgg ccatcatcgt gtcgcggtac
     8281 tcgatggcgg cctgcaggcc tggatggcgg ccgggctcgc gctcgacgac gagccgccgc
     8341 gtgcgcgccc cgcgacctat acgctggcgc tgcgtccgga ccgggtcgat acccagtacg
     8401 tgcagcaaca tctcggccgc cccgacatgc tgctgctcga tgcgcgcagc cccgaccgct
     8461 tccgcggcga gaacgaggtg ctcgatccgg tgggcgggca catccccggc gcacgcaacc
     8521 gcttcttccg cgacaacctc gacgcgcgcg gctgcttcaa gcaggcctcg gtactgcgcg
     8581 aagagttcgc cagcctgctc ggcgcataca cgccgcgcca ggtggtgcac cagtgcggct
     8641 cgggcgtcac cgcctgcgtg aacctgctcg cgatggaggc cgccgggctg accggctcgc
     8701 gcctgtatgc gggttcgtgg agcgagtggt gcgcagatcc cacgcggccg atggcacgcg
     8761 gcccggcctg aaagcgcgcg ggccgcccag ggcggcccgg acctccccgc gtcgcaagcg
     8821 acgcactacc aatcgacctt ctccaccgca ccgagcgaac gccccagaaa gcgctcgccg
     8881 atggtctgga agcgcagcgg cacgtcgctc accacgaagc ggtaatctgc gacgcccccg
     8941 ccaccgcgcg ccagcccgga cagctgcagg cgctcggcgg ccaactccgc ggtggtcagc
     9001 gccgagtcga tcaggcgcac gcgcgccccg gcgacgtcgc gtagcagggg cttgagcagc
     9061 gggtaatggg tgcagcccag cacgaggctg tcgacctcct cggcgagcac cgggcgcagg
     9121 tattcctgcg cggtgaggcg ggtcacctcg tggtccagcc agccttcctc gaccagcggc
     9181 acgaacagcg ggcaggcctg ggaatacacc cgcaccgccg gatcgagttc gtgcatgcgg
     9241 cgcgcgtagg cgttgctgtt gatcgtggtc ggcgtgccaa tgacaccgat gcgccgtccc
     9301 ggcgaggccg ccaccgcggc gcgcgcgccg gcctcgatga cgtcgagcac cgggatgccg
     9361 gcggccagcc ggtgcaccac gtccgccgcc accgccgcca tggtgttgca cgcgatgatc
     9421 agcatcttga cgtcgcgctg cagcaggaat tcggtgatct gcgcggtgaa gtgctcgatc
     9481 gtggcgaccg acttcacccc gtagggcacg cgcgcggtgt cgccgaagta gacgatgtcc
     9541 tcgagcggca ggcgctccat cagcgcgcgc accacggtga gcccgccgac gccggaatcg
     9601 aacaccccga tcgggcgcgc agccgcgctc atcgcccgcc cccgctgcag gggacgagat
     9661 cggtcaagac agcaccaccg aggcgaactt gcgcttgccc acctgcagca ccacggtctc
     9721 gccggcacgc agcagcaggc ccttgtcctc ggcgcgctcg ccgtcgatgc gcaccccgcc
     9781 ctgctccacc atgcgcatcg cctcggaggt ggtgccggtg agcccggcct gcttgagcac
     9841 ctggaacagc ggcaggccgc cggcgcccac gttcacattg acctgcggga tgtcatcggg
     9901 gatcgcgttg cgctggaagc gtgcctcgaa gtcggcaagc gcctcctcgg ccgcacgctg
     9961 gccgtggaag cgggcgacga actccatcgc cagcatcacc ttgacgtcgc ggggattgcg
    10021 cccgccctcg acgtcggcgc gcagctgcgc gatctcggcg ctggagcgga aggacagcag
    10081 ctcgtagtag cgccacatca gggtgtcgga caccgacatg gtcttgccga agatctcctt
    10141 ggccggctcg gcgatgccga tgtagttgcc gagcgacttg gacatcttgt tgacgccgtc
    10201 gaggccctcg agcagcggca ccatcaccac gcactgcggc tcctgcccgt aatgcttctg
    10261 cagctcgcgg cccatcagca ggttgaagcg ctggtcggtg ccgccgagtt cgacgtcggc
    10321 cttcatcgcc accgagtcgt agccctggca cagcgggtag aggaactcgt ggatcgcgat
    10381 cggctggtta ttgccgtagc gcttggcgaa gtcgtcgcgc tcgagcatgc gcgccacggt
    10441 ctgctgcgcg gccaggcgga tcatgccggc ggcgccgaga tcgttcatcc aggccgagtt
    10501 gaagcagatc tcggtcttct ccgggtcgag gatcttgaac acctgctcct ggtaggtcct
    10561 ggcgttctcg aggatctgct cgcgcgagag cggcgggcgc gtgctgttct tgccgctggg
    10621 gtcgccgatc atgccggtga agtcgccgat caggaactgg acctggtgac cgagttcctg
    10681 gaagtgacgc atcttgttga tcaggaccgt atggccgaga tgcagatcgg gcgcggtggg
    10741 gtcgaagccg gccttcaccc gcaggggctt gccgcgcttg agcttctcga ccaattcggc
    10801 ctcgatcagc acggcgtcgg cgccgcgctt gatgaggtcg atcgccgcct gaacatcaga
    10861 cattcaattt ctccaaaatt actttgccga accgaagcgc acctttgcta cacttccgcg
    10921 acttttttcc acatcccagc ccaccgagac catgaaggcc agaaagaccc gaattctagc
    10981 cgatctcccg gctcgtctcc tctcccgccc gcgaacctgg gttgccgccg gtatcaccgg
    11041 ggtttccatg ctcggggtgg tcgccgcgac cgcggtcgca cccggcagcc tgccttcgga
    11101 cctgctcgtc gagcgcgtcg tcgaggccct gcccgcgccg gcggtcacgc gcaccggcac
    11161 cgacgcctcg ctacccttcg tccattacga acgcatccag gccggcgaca cgctgcaggc
    11221 gatgttcaac cgcctgcgca tcgacgacgc cgaggcgctc gcccacctct ccgcgtccga
    11281 tgccggccgc aaggcgctgc gccagctgcg cgcgggccgc tcggtcaccg cggtggtgtc
    11341 gccgcaaggc cgtctgctca gcttctcgct gccggtcggc aacacggacg ccgaggtcgt
    11401 cctcgaacgc gacgacgccg gcctcgcgct gcgtgaatcc agcggcgcca cccagaccac
    11461 gatggtcgag atgcgctcgg gtacgatcac ccactcgctt ttcggcgcca ccgacgacgc
    11521 cggcctgccc gacaacgtgg cgacccagct cgccaccatc ttcggcacct ggatcgactt
    11581 ccacaccgac ctgcgcaagg gcgaccgctt caacgtggtc tacgaggcca tctacgagga
    11641 aggcaatccg gtgcgcgccg ggcgcatcct cgcggccgaa ttcatcaaca atggcgaccg
    11701 ccacgcggtg gtgctgtatc gcggtccgag cggcaaggag caatactaca gcgacgacgg
    11761 cgagagcctg cagcagggct tcctgcgctc gcccctggag ttctcgcgcg tcagctcgaa
    11821 cttcggccgc cgcctgcatc cgatccacgg caactggcgc gaccacaacg gcaccgactt
    11881 ctcggcaccc gtcggcaccc cggtgatggc cacctcggac gccaccatcg agttcatcgg
    11941 cacccagcgt ggcttcggca acctgatcgt gctcaagcac cgcagcgata tcaccaccca
    12001 ctacgcccac ctcaacggct tcgccaaggg cctcgccaag gggcaggcgg tgagccaggg
    12061 cgatctgatc ggctacgtcg gctgcacagg ctggtgcacc gggccgcacc tgcactacga
    12121 ggtgcgcctg aaggacgtcc ccgccgaccc gatgacggtg gcgctgccga tggccgactc
    12181 cttcaatgac cgggaactcg ccgccttcaa gcgcaacacc gcgcatctgc gccagcgctt
    12241 cgcgctgctc aactacgaac tcgccgacgc acgctgagca ccgcacggcc ggaccaaagc
    12301 aaacggggcg ccgcggcgcc ccgttttgtt tgcgacaacc gcgttcacac cgtgaacgac
    12361 gagccgcagc cgcaggtgct ggtcgcgttc ggattgcgga tcacgaactg cgagccttcc
    12421 aggccttcgg tgtagtcgat ctccgcgccc accaagtact ggtagctcat cggatcgacc
    12481 agcagcagca cgccgttctt ctcgaacgtg gtgtcgtcct cgttcacttc ctcgtcgaaa
    12541 gtgaagccgt actgaaagcc cgaacacccg ccgcccgaca cgaagacgcg cagcttgagg
    12601 tcgggattgc cttcctcttc gatcagctcg cgaaccttgt tcgcggcgct atcggtaaag
    12661 accatgattt ccggggttgc gacttctgcg ctcatgtttt ctcctctgct ggggaacacc
    12721 gcggatgatg cgccttccgc ggttgaatcg tcaaaatgtg atcgaatccg gtcgtaacag
    12781 ttccctcagg ggctgaccgg cacgatgtcg agcccggttt cctcgtcgaa accgaacatg
    12841 atgttgaggt tctgcaccgc ctggcctgcc gcgcccttga ccagattgtc gatcaccgac
    12901 aacaccacca cggtgtcgcc gccctgcggg cgatggaccg cgatgcggca caggttggag
    12961 gcgcgcaccg agcgcgtctc gggatggctg cccgcgggca tgacgtcgac gaaggcctcg
    13021 ccagcatagc gcttctcgta cagcccctgc aggtcggcag cgtcgacccc cggcttcaga
    13081 cgaccgtaca gcgtggcgtg gatgccgcgg atcatcggcg tcaggtgggg cacgaaggtc
    13141 agcccgaccg cgtgcccggc ggccagagcc agcccctgac ggatctccgg cagatggcga
    13201 tggcccggca cgccataggc cttgaaggag tcggcggcct ccggaagcag ggtgtgcacc
    13261 tcggccttgc ggccggcgcc cgagacgccc gacttggcat cggcgatcag gtgatcggtg
    13321 tcgatcagcc cggcctcgac caagggcagg aagccgagct ggaccgcggt ggggtagcag
    13381 cccgggttgg cgagcacgcg cgcgccgcgg atctgcgcgc gattgacctc gggcaggcca
    13441 tacaccgcct cggcgacgag ttccggcgcc gcatgctcca tgccgtacca cttctgccac
    13501 tcggcgacgt cgcggatgcg gaagtcggcg gcgaggtcga tcacccgcac cccggcagcg
    13561 accaactcgg ccgcctgctt catcgcgatg ccgttgggcg tggcgaagaa caccgcgtcg
    13621 cacttctcca gggcggcgtc ctgcggggtg acgaacttga gatcgacccg gcggcgcagg
    13681 ctggggaaca tgtcggagac cgccatgccg gcctcgccgc gcgaggtgat cgcggtcagc
    13741 tcgaccccgg ggtggcgcgc cagcagacgc aagagctcga cgccggtata tccggtaccg
    13801 cctacgattc caaccttgac catgctcgtt cctcgcgacg aaagactgaa ccgcgattat
    13861 ctcatcatcg gccgacgcgg gcggtgaacg cctcgttacg gctgccgcac cccgaactgc
    13921 aagaacgcgt gcgagcggga cacacaagca cgaaagccgc ccaaggggcg gcttccggac
    13981 tgcgatgcgc gataaagaga atcagcgctt cgagaactgc ttggcgcgac gggccttgcg
    14041 caggccgacc ttcttgcgct cgacctcgcg ggcgtcgcgg gtcacgaaac cggcggcacg
    14101 caggtcgccc ttcaggccgg cgtcgtaatc gaccagcgcg cgggtgatgc cgtgacgcac
    14161 cgcacccgcc tgaccggatt cgccaccgcc gacgacgttg accatgatgt cgaaacggcc
    14221 ttcgttgccg gtgaggacca gcggctggcg cacgatcatg cggccggttt cacgcgagaa
    14281 gaactcgtcg accggcttgc cattgacgac gatgttgccg gtgcccggct tgatgaacac
    14341 gcgagcgacc gcagtcttgc ggcggccggt accgtagttg taagtcacag ccattgtcgg
    14401 ctccttcaga tctcgagaac ttgcggctgc tgagcggtgt gcggatgcga cggccccgcg
    14461 tagcacttca gcttcttcag catggcgtag cccagcgggc ccttcggcag catgcccttc
    14521 accgccttct cgagcacgcg ctcggggaag cgctgctgga gcttggtgaa gttggtttcg
    14581 tagataccgc ccgggtagcc cgagtgacgg tagtacttct tgtcctgcgc cttgttgccg
    14641 gtcacgcgca gcttttcgac gttgaccacg acgatgaagt cgccggtgtc cacgtgcggg
    14701 gtgtagatgg ccttgtgctt gccacgcagg cggcgggcga cttcggcggc gagacggcca
    14761 agcaccttgt ccgtgccgtc gacaacgaac cagtcgcgct tgacctcgtg cggcttggcg
    14821 gaaaacgtct tcatgtgaat cctcgatctt tgtcgggccg tcggagatgg ccgtcaaacg
    14881 gaaagctcgc aatcctactt ccaacacatg aatcgtgtca atcgtcgaag cgcgaacgcg
    14941 ggcggagaac aaaaaaaacg cagtccgatc ggactgcgtt aaatccacac caaaggagga
    15001 gggtggagga gacacggctt ggatcggcgc gaccccggac gtgggagtcg atgcgtctgc
    15061 gatccgactg gaacggatta tgcatagcta ccccgggctg tgcaaggcat ttttgtgcga
    15121 cgcggaagaa aagttttgat tgattgatca gctcgcttta tcgcaaagca gctttttgtt
    15181 ttaaatcaat tttcgctctt ttcgcgacct ccaagcccct cacccggacg cggacacccc
    15241 cctccctgcg ccccgttttg ctgcagtgca tcactacgga gccgtattga acccggccgc
    15301 gctcgcctcg ggcccggaag tttcaaccgc aagtcggcac gggcacgaag gatgcggcga
    15361 cgccaggcga ggcgcacgaa ctccatgacg cactgcggta acatgccgcc ccaaccccca
    15421 acgaacccga caacgggacc agaacccgaa ggagcgaaca gcatggaatg cacggtcaag
    15481 tggctcgacg gcatgagctt catcgccgaa accggcaccg gccacctcgt cgcgatggac
    15541 ggcgcgcccg atgccggcgg gcgcaacctg gctccacggc cgatggagtt gctgctggcc
    15601 ggcgccggtg gctgcaccgc cttcgacgtc atgctgatcc tcaagcgcgg acgccacgat
    15661 gtgcgcggct gcagcgtgcg cctcgaagcc gagcgcgcgg agtccgaccc caaggtgttc
    15721 acccgcatcc gcttcatcta cacgatcacc ggtcgcaagc tcaagcgcga gacggtcgag
    15781 cgcgcggtgc acctgtcggc ggaaaaatac tgctcggcct cgatcatgct cggcaagacc
    15841 gccgagatga tccacgaggt cgagctggtc gaggcggact gaggaccgcg gcggcgcgag
    15901 gctgccctcg cgacgcggac gagggcacgg gaatcgctca gagccggtag gcgagcgtcg
    15961 tcatcacccg cgcggcgagc ttcatcgccc ccttcgccgg tgccggcaac tcgtgcgcac
    16021 ccaggcgcag cgccgtcgcg gcgtgctcgg cctcgtccac cttcatctgc tcgacgatcg
    16081 cgcgcgagcg ccgatccgcg gccggcaggg tgtcgagatg gctcttgagg tgctgctcga
    16141 cctggcgctc ggtctcagcc aggaagccga ggttccagcg ctcgccgaag cgccccgccg
    16201 ccagcccgag ggcgagcgcg ccgccatacc acagcggatt gagcaggctc ttgcgaccac
    16261 cgagctcggc gatgcggcgc tcggtccacg ccaggtgctc ggtctcctcc tgcgcggcct
    16321 ggcgcagggt gtcgcgaatc gcgggctcgc gcgacatgat ggactgcccc tggtagagcg
    16381 cctgcgcgca cacctcgccg acatgattca cccgcatcag cgccgccgca tggctgcgct
    16441 cggctgcgcc cagctcggca tcgggcacgc cctcgcccgg catcgcccgc acgctgcgcg
    16501 ccgacgcgaa gaccgtccgc agggccttgt cgaactcgat gatggcctga tcgatcattg
    16561 atgcactcca gacgggtcga tggggtcgcg ccgctcccgc agcaggcgca acgcatgctc
    16621 gcggtcgcgc ggcccggccg ggccgtcgca gaaatagtcg tgcgccagcg ccgcgcgcgg
    16681 gcggttgtag ccgctttcga cgatgggctg ccaggcactg tttttcagcg ccgcccaggt
    16741 cccgtccttg cgtccgctgg cgtagatccg gcgctcgaag gtggcgcagc ggatgccctc
    16801 gaagctgacg ttctccgcgc cgccgcgcgt gcgtacgacc aaggtgtagc gcaccacgcc
    16861 atccgccccg acgccgacgc tgtcctcgtc gacgaagaag gcattgggag aaggcgtgcc
    16921 gacgtcgatc gcacgcagcg cggccgcgcg cggcgcggcc ggcatcgtcg cctcgccctc
    16981 cttccagtcg ggatcggcat ccgacaggaa ccccgcggac gcgggcgccg acaggagggc
    17041 gagggcgaca acgccggcgc gtggaagtgc accgagcgag gggcgcaaga aacggatcgg
    17101 gaaaggactc aagagtagga gctccgcatc ggggaggaag gaagcgggca gcgccgaagc
    17161 aggaccggcg cgcccacggc acacggtccc gccgcggcgg gcacgcgccg gccgcacggg
    17221 gccagcccgc cgccggcctg cgccgtgcgg caggacggag tcgggctact tccgcatcgc
    17281 atcgcgcagt ccgtgctcgg cgcgctcgcc gagcaccacc cgcaccggct cgggagcggg
    17341 ttccggttcg ggcagtcgat agctgtccgg cagctcgacg ctgcgcgaac gaaacaggat
    17401 gcgcgccagt tcgatcaagg ccagctgata cacctggcgc ttgaattcga tcaccgcttc
    17461 cagcggcacc cagtagttgc tccagcgcca ggcatcgaat tccggatgag tgctcgcgcg
    17521 cagacagacg tcggagtcgc gccccaccag gcggagcagg aaccagatct gcttctgccc
    17581 acggtaggtg ttgcgccact cgcgccggat ccagtgcttg ggaacctcat agcgcaacca
    17641 tccgcgcgta cggccgagga tcctgacgtg ctcgggccgc aggccgacct cttcgtggag
    17701 ttcccgatac atggcctgct ccggcgactc gccgtgcttg atgccacctt gtggaaactg
    17761 ccaggaatgt tcgcggatgc gcttgcccca gaagacctcg ttgcgcgcat tgaccagaat
    17821 gatgccgacg ttcgggcgaa agccttcacg gtcaagcatg acgaaccttc gaattcgttt
    17881 gattggtcac gattttttca cacttggcga cgcaatgaaa gcgcactgcc acggtgcatc
    17941 cactaagctg gaggtcgagg aacgcttgcg cgccggcccc atgcgctaga atcgcgcgct
    18001 tgtccacgcc tgatccgaag actcccatgc gcgccagcca gttccatctc ttcaccctca
    18061 aggaagcccc ctccgacgcc gaggtcgtca gccagaagct gatgctgcgc gccggcatga
    18121 tccgcaaggt cgccgccggc atctacagct acatgccgat gggcctgcgc gcgatccgca
    18181 aggtcgaggc catcgtgcgc gaggagatgg accgcgccgg cgccatggaa ctgatcatgc
    18241 cgatggtgca gcccgccgag ctgtgggacg agaccgggcg ctgggacaag atgggcgacg
    18301 agctgctgcg cttcaaggac cgccacgagc gcgacttcgc gctgcaaccc acctccgagg
    18361 aggtcgtcac cgacatcgcg cgccaggagc tcaagagcta ccgccagctg ccgaagaact
    18421 tctaccagat ccagaccaag ttccgcgacg aacgccggcc gcgcttcggc gtcatgcgcg
    18481 ggcgcgaatt caccatgaag gacgcctact ccttcgaccg cagcgaggaa gccgccgggc
    18541 gcagctacga catcatgttc gccgcctaca agcgcatctt cgaccgcctc ggcctcgaat
    18601 accgcgcggt ggcggccgac accggcgcca tcggcggcga ccgctcgcac gagttccagg
    18661 tcatcgccga caccggcgag gacgccatcg tctactgccc cgactccgac tacgctgcca
    18721 acatcgagct cgccgaggcg gtgtccctgc tcgcccgccg cgcagagccc gcccgggcgc
    18781 tggcaaaaac ccccacaccg ggcaaggcga cctgcgagga cgtcgccgcg ctgctgggcg
    18841 tgccgctggc gaccacggtg aagtcgctgg tactcgccac cgacgatgtc gatgaaaagg
    18901 gcaagcccgc cggcgtcacc gtgtggctgc tgctggtgcg cggcgaccac gccctcaacg
    18961 aggtcaaggc cggcaagctg gaaggcctca aggcaggctt ccgcttcgcc accgaggccg
    19021 agatcctcga acacttcggc tgcaagccgg gctacctcgg ccccatcggt ctgaagaagc
    19081 cggtcaaggt catcgccgac cgcacggtcg cccacatggc cgacttcatc tgcggcgcca
    19141 acgaggccga cttccacttc accggcgtga actggggccg cga
//
LOCUS       DAOQBS010000054        18866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2055_length_18866_cov_13.280793, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000054 DAOQBS010000000
VERSION     DAOQBS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 18866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18866
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2055_length_18866_cov_13.280793"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /gene="tuf"
                     /locus_tag="PKN25_07875"
     CDS             <1..290
                     /gene="tuf"
                     /locus_tag="PKN25_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNV90854.1"
                     /translation="TPHTHFTGEIYVLSKEEGGRHTPFFNNYRPQFYFRTTDVTGSIS
                     LPEGTEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE"
     gene            378..689
                     /gene="rpsJ"
                     /locus_tag="PKN25_07880"
     CDS             378..689
                     /gene="rpsJ"
                     /locus_tag="PKN25_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNV90855.1"
                     /translation="MQNQKIRIRLKAFDYRLIDQSALEIVDTAKRTGAVVRGPVPLPT
                     RIERFDLLRSPHVNKASRDQMEIRTHQRLMDIIDPTDKTVDALMKLDLPAGVDVEIKL
                     Q"
     gene            802..1440
                     /gene="rplC"
                     /locus_tag="PKN25_07885"
     CDS             802..1440
                     /gene="rplC"
                     /locus_tag="PKN25_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNV90856.1"
                     /translation="MSLGLVGRKVGMTRIFAEDGKTIPVTVLDVSDNRVSQIKTIDTD
                     GYAAVQIAYGKRRASRITKPVAGHLAKAGVEPCRGIKEFRVEAEQAGSMNAGDAVGAD
                     LFQVGQKVDVTGVTIGKGFSGPIKRHNFSSNRASHGNSISHNSPGSIGMAQDPGRVFP
                     GKRMAGQYGNVQRTTQGLEIVRVDVERQLLLVKGAVPGAKGGDVVVRPAVKA"
     gene            1451..2071
                     /gene="rplD"
                     /locus_tag="PKN25_07890"
     CDS             1451..2071
                     /gene="rplD"
                     /locus_tag="PKN25_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNV90857.1"
                     /translation="MELKLLNDQGQAASTLEASDVLFGRDYNEALVHQVVTAYLANAR
                     SGNRKQKGRSEIAKSTRKPFRQKGTGNARAGMASSPLWRGGGKIFPSSPDENFSQKVN
                     RKMYRAGVAAILSQLAREDRLAVVENFSVEAPKTRLLSQKLKGMGLDSVLVITDEIDE
                     NLFLSSRNLHQVLVLEVSEADPVSLVRFPKVLVTKGALAKMEEAWQ"
     gene            2068..2373
                     /gene="rplW"
                     /locus_tag="PKN25_07895"
     CDS             2068..2373
                     /gene="rplW"
                     /locus_tag="PKN25_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNV90858.1"
                     /translation="MSAISQERLLQVLLAPQISEKATFVADKNEQVVFKVASDATKPE
                     VKAAVESLFKVEVKSVQILNVKGKSKRFGKTMGRRKDWKKAFVCLKPGQEINFAAGE"
     gene            2380..3207
                     /gene="rplB"
                     /locus_tag="PKN25_07900"
     CDS             2380..3207
                     /gene="rplB"
                     /locus_tag="PKN25_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNV90859.1"
                     /translation="MAIVKLKPTSAGRRAQVKVVNPDLYKGRPVDALIEKQTKNAGRN
                     NYGRVTMRHQGGGHKQHYRLVDFRRNKDGIPAKVERIEYDPNRSAHIALLCYADGERR
                     YIIAPRGVEVGQQLISGAEAPIKAGNALPIRNIPVGSTIHCIEMVPGKGAQLARAAGT
                     SVQLLAREGSYAQLRLRSGEIRRVHVECRATIGEVGNGEHGLRKIGKAGANRWRGIRP
                     TVRGVAMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRSNKRTDGMIVQRRHKR"
     gene            3218..3493
                     /gene="rpsS"
                     /locus_tag="PKN25_07905"
     CDS             3218..3493
                     /gene="rpsS"
                     /locus_tag="PKN25_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNV90860.1"
                     /translation="MARSIKKGPFIDAHLLKKVDAARTSNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGKQHIPVFVTENMVGHKLGEFALTRTFKGHAASKKAKR"
     gene            3503..3832
                     /gene="rplV"
                     /locus_tag="PKN25_07910"
     CDS             3503..3832
                     /gene="rplV"
                     /locus_tag="PKN25_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNV90861.1"
                     /translation="METKAILRGVRLSAQKGRLVADLVRGMPVGRALDTLAFSPKKGA
                     KIIRKVLESAIANAEHNDGADIDALKVKTIHVEEGMTLKRFTARAKGRGNRILKPTCH
                     VYLTVGE"
     gene            3842..4612
                     /gene="rpsC"
                     /locus_tag="PKN25_07915"
     CDS             3842..4612
                     /gene="rpsC"
                     /locus_tag="PKN25_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNV90862.1"
                     /translation="MGQKIHPTGFRLAVTRDWGSRWFANSKDYAGMLHEDLKVRDYLK
                     KRLAHASVGRVVIERPAKNARVTLYSARPGVVIGKKGEDIEALKRDLQKIVGVPVHVN
                     IEEVRKPELDAKLIADSIAQQLEKRIMFRRAMKRAMQNAMRLGAQGIKIMSSGRLNGA
                     EIARTEWYREGRVPLHTLRANIDYGVSEAKTTYGVIGIKVWVFKGEMLGRNEQPVAAE
                     PEADNRRGRRGAPRDGEGRPGRRPARRGEGRQEESRSE"
     gene            4612..5028
                     /gene="rplP"
                     /locus_tag="PKN25_07920"
     CDS             4612..5028
                     /gene="rplP"
                     /locus_tag="PKN25_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNV90863.1"
                     /translation="MLQPTRRKYRKEQKGRNTGVATRGAKVSFGEYGLKAIGRGRLTA
                     RQIESARRAMTRHIKRGGRIWIRIFPDKPISKKPAEVRMGNGKGNPEYWVAEIQPGKV
                     LYEMDGVDEALAREAFRLAAAKLPIETVFVTRLIGG"
     gene            5031..5225
                     /gene="rpmC"
                     /locus_tag="PKN25_07925"
     CDS             5031..5225
                     /gene="rpmC"
                     /locus_tag="PKN25_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNV90864.1"
                     /translation="MKATELRAKSADELNKELIELLKAQFSLRMQHATQQLGNTSQIG
                     KVRRDIARVRTILREKAGQQ"
     gene            5222..5491
                     /gene="rpsQ"
                     /locus_tag="PKN25_07930"
     CDS             5222..5491
                     /gene="rpsQ"
                     /locus_tag="PKN25_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNV90865.1"
                     /translation="MSENVEKVRRALVGRVVSDKMQKTVTVLVERRVKHPLYGKIITR
                     SAKYHAHVEDGVAAQGDLVEIEECRPISRTKTWRVAKVLEKATII"
     gene            5648..6016
                     /gene="rplN"
                     /locus_tag="PKN25_07935"
     CDS             5648..6016
                     /gene="rplN"
                     /locus_tag="PKN25_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNV90866.1"
                     /translation="MIQMQSKLDVADNSGARSVMCIKVLGGSKRRYAGVGDIIKVTVK
                     DAAPRGRAKKGDVYNAVVVRTAKGVRRPDGSLIKFDSNAAVLLNNKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6028..6345
                     /gene="rplX"
                     /locus_tag="PKN25_07940"
     CDS             6028..6345
                     /gene="rplX"
                     /locus_tag="PKN25_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNV90867.1"
                     /translation="MNKIRKGDEVLVLTGKDRGRRGTVLRRVDDERVVVEGVNRVKKH
                     VRPNPLKGEVGGIVEKEMPIHVSNVALFNPATQKGDRVGIKTLEDGRKVRFFKSNGEL
                     VNA"
     gene            6355..6894
                     /gene="rplE"
                     /locus_tag="PKN25_07945"
     CDS             6355..6894
                     /gene="rplE"
                     /locus_tag="PKN25_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNV90868.1"
                     /translation="MARLQQVYKDEVAPALQQQFGYKSVMEVPRITKITLNMGVGEAV
                     GDKKILEHAIGDMTKIAGQKPVSTKARKSIAGFKIRDGYPIGCMVTLRGPRMFEFLDR
                     LITIALPRVRDFRGIAAKGFDGRGNYNLGVKEQIIFPEIEYDKIDALRGMNISITTTA
                     KTDEEARALLGAFKFPFKN"
     gene            6902..7207
                     /gene="rpsN"
                     /locus_tag="PKN25_07950"
     CDS             6902..7207
                     /gene="rpsN"
                     /locus_tag="PKN25_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNV90869.1"
                     /translation="MAKLALINREAKRAMLVEKYASKRAALVAKVKDTSLSEEERMAA
                     RLALQQLPRNANPTRQRNRCALTGRPRGVFRKFGLCRNKLREIAFRGEVPGMTKASW"
     gene            7221..7616
                     /gene="rpsH"
                     /locus_tag="PKN25_07955"
     CDS             7221..7616
                     /gene="rpsH"
                     /locus_tag="PKN25_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNV90870.1"
                     /translation="MSMSDPIADMLTRIRNGQQAQKASVSMPSSKLKVAIAKVLQDEG
                     YIDGFAVRDEAGKASLDVTLKYYAGRPVIERLERVSRPGLRIYKGSDDLPRVMNGLGV
                     AIVSTPRGVMTDRSARANRVGGEVICLVA"
     gene            7627..8160
                     /gene="rplF"
                     /locus_tag="PKN25_07960"
     CDS             7627..8160
                     /gene="rplF"
                     /locus_tag="PKN25_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004738786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNV90871.1"
                     /translation="MSRVAKNPVAIPQGVEVMISDAEIAVKGPLGTLRQPLSGEVSVV
                     REGDAVVCKAREGAENARAMSGTVRALVNNMVTGVTKGFERKLTLVGVGYRAQAQGDK
                     LNLSLGFSHPVVHQMPEGVKVETPTQTEIVIRGSDKQKVGQVAAEVRAYRAPEPYKGK
                     GVRYSDEVVVLKETKKK"
     gene            8172..8525
                     /gene="rplR"
                     /locus_tag="PKN25_07965"
     CDS             8172..8525
                     /gene="rplR"
                     /locus_tag="PKN25_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNV90872.1"
                     /translation="MNKKETRLRRARKTRAKIAELKAVRLTVFRSNCHIYAQIISGCG
                     SRVLAAASTVEPDVRAQVANGGNKAAAEAVGKLIAERAKAAGVEQVSFDRSGFLYHGR
                     VKALAEAAREGGLKF"
     gene            8538..9062
                     /gene="rpsE"
                     /locus_tag="PKN25_07970"
     CDS             8538..9062
                     /gene="rpsE"
                     /locus_tag="PKN25_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNV90873.1"
                     /translation="MAKQQTKRAQASDERDDGLKEKMVAINRVTKVVKGGRILGFAAL
                     TVVGDGDGAIGMGKGKSREVPVAVQKAMDEARRKMRKVSLKNGTLQHTVMGKHGAASV
                     LMQPAPAGTGIIAGGPMRAVFEVMGVTDVICKCIGSANPYNVVRATINGLMAINTPAE
                     IAAKRGKSVEEILG"
     gene            9066..9248
                     /gene="rpmD"
                     /locus_tag="PKN25_07975"
     CDS             9066..9248
                     /gene="rpmD"
                     /locus_tag="PKN25_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNV90874.1"
                     /translation="MSDKKIKVTLVKSVIGTKQSHRATVRGLGLRRLNHSVELADTPE
                     VRGMINKVSYLVKCEG"
     gene            9250..9687
                     /gene="rplO"
                     /locus_tag="PKN25_07980"
     CDS             9250..9687
                     /gene="rplO"
                     /locus_tag="PKN25_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNV90875.1"
                     /translation="MRLNSIKPGEGSKKEARRVGRGIGSGLGKTCGRGHKGQKSRAGG
                     FHKVGFEGGQMPLQRRLPKRGFVSLTRARNVEVRLSELDKLPVDEVDLLTLKQAGVIA
                     ADALSAKVVLSGSISRKVVLRGVGATQGAKAAIEAAGGSVAAE"
     gene            9703..11028
                     /gene="secY"
                     /locus_tag="PKN25_07985"
     CDS             9703..11028
                     /gene="secY"
                     /locus_tag="PKN25_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNV90876.1"
                     /translation="MANPAATLGSPGRFGDLKRRLLFLVGALIVYRIGAHIPVPGIDP
                     QGLAELFQSQQGGILGVFNLFSGGALSRFTIFALGIMPYISASIIMQLMTVAVPSLEA
                     LKKEGEAGRRKITQYTRYGTLVLALAQALGIAIALEGQAGLVLEPGLMFRFVTVATLV
                     TGTMFLMWLGEQITERGIGNGISMIIFAGIIAGLPAAIGGLFELVRTGAMHPFTALFI
                     CILVVLVTAFVVFVERGQRKILVNYAKRQVGNKVYGGQSSHLPLKLNMSGVIPPIFAS
                     SIILFPATLGQWFGSSEGMYWLRDIASTLSPGQPIYVMLYAVAIVFFCFFYTALVFNA
                     RETADNLKKSGAFVPGIRPGDQTARYIDKILMRLTLAGAIYITLVCLLPEFLILKWNV
                     PFYFGGTSLLILVVVTMDFMAQVQAFVMSHQYESLLKKANFKGAGLPGR"
     gene            11033..11251
                     /gene="infA"
                     /locus_tag="PKN25_07990"
     CDS             11033..11251
                     /gene="infA"
                     /locus_tag="PKN25_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNV90877.1"
                     /translation="MTKEDVIEMQGEVTENLPNATFRVRLENGHMVLGHISGKMRMHY
                     IRILPGDKVTVQLTPYDLTKGRIVFRTK"
     gene            11277..11390
                     /gene="rpmJ"
                     /locus_tag="PKN25_07995"
     CDS             11277..11390
                     /gene="rpmJ"
                     /locus_tag="PKN25_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNV90878.1"
                     /translation="MRVQASVKRLCRNCKIIRRKGVVRVICSDPRHKQRQG"
     gene            11444..11806
                     /gene="rpsM"
                     /locus_tag="PKN25_08000"
     CDS             11444..11806
                     /gene="rpsM"
                     /locus_tag="PKN25_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNV90879.1"
                     /translation="MARIAGVNIPNHKHAEIALTAIYGIGRSRAQKICDAAGIVRSVK
                     IKDLTESDMEKLRDEVARFVVEGDLRREVTMNIKRLMDLGCYRGVRHRRGLPLRGQRT
                     RTNARTRKGPRKPIAGKK"
     gene            11819..12208
                     /gene="rpsK"
                     /locus_tag="PKN25_08005"
     CDS             11819..12208
                     /gene="rpsK"
                     /locus_tag="PKN25_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNV90880.1"
                     /translation="MAKTGTKVRKKVKKNVAEGIAHVHASFNNTIITITDRQGNALSW
                     ATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQECGIKNLEVRIKGPGPGRESSVRALNA
                     LGIKISSITDITPIPHNGCRPPKKRRI"
     gene            12223..12852
                     /gene="rpsD"
                     /locus_tag="PKN25_08010"
     CDS             12223..12852
                     /gene="rpsD"
                     /locus_tag="PKN25_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNV90881.1"
                     /translation="MARNLDPKCRQCRREGEKLFLKGEKCFTDKCSIERRAYAPGQHG
                     QRSGQRLSGYGVQLREKQKIRRIYGVLERQFRKVYAEADRRRGQTGENLLQLLEGRLD
                     AVAYRMGFGASRAEARQVVRHNGVLVNGKRVNIPSYVVRPGDVVELTERARGHLRVKA
                     ALEAAESRGFPEWLEVDAKAGKGTFKAYPVRSELPPTINEGLVVELYSR"
     gene            12886..13872
                     /gene="rpoA"
                     /locus_tag="PKN25_08015"
     CDS             12886..13872
                     /gene="rpoA"
                     /locus_tag="PKN25_08015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNV90882.1"
                     /translation="MLMQSNALLKPRIIDVQSTSPVQARVTMEPFERGFGHTLGNALR
                     RILLSSLPGHAPTEVTIEGVLHEYSTLDGVREDIVDLLLNLKGVVFKLNGRSEVTVRL
                     AKAGEGVVTAADIEVGHDVEIINPDHVIAHLAPGGKLDMQIKVEEGRGYVPGNSRPEN
                     AESKSIGRVVLDASFGPVRRVSYLVESARVEQRTDLDRLIIDIETNGAVDPEEAIRYA
                     ARVLMDQLSVFADLEGTAPERVEAASPTIDPVLLRPVDDLELTVRSANCLKAENIYYI
                     GDLIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPAGLEKLG"
     gene            13898..14296
                     /gene="rplQ"
                     /locus_tag="PKN25_08020"
     CDS             13898..14296
                     /gene="rplQ"
                     /locus_tag="PKN25_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNV90883.1"
                     /translation="MRHRNGLRKLNRTSSHRQAMFRNMANSLLRHELIKTTLPKAKEL
                     RRVVEPMITLGKKPSLSNRRLAFNRLRDRDMVVKLFDELGPRYATRNGGYLRILKCGF
                     RDGDNAPMAYVELLDRPDVEAVEVEEAEAA"
     gene            14407..15429
                     /gene="galE"
                     /locus_tag="PKN25_08025"
     CDS             14407..15429
                     /gene="galE"
                     /locus_tag="PKN25_08025"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNV90884.1"
                     /translation="MAVTLVTGGAGYIGSHTCVELLGAGQDVVVVDDLSNASFEAVRR
                     IEELGGKPLTGFVEGDVRDHGVLARVFGEHEVAAVIHFAAKKAVGESVSQPLAYYDNN
                     LHGLLSVLQAMDAAGCRRMVFSSSATVYGDPASVPIREDFPTTATNPYGWTKLMGEQI
                     LRDLAASDPRWNVVLLRYFNPVGAHASGRIGEDPDGLPNNLMPFVSQVAVGRLPRLRV
                     FGDDYATPDGTGVRDYIHVVDLALGHLRALERIDELPGVTTLNLGTGRGYSVLEMVRA
                     FEAASGRPVPYDIVARRPGDIAACWADPARAEQVLGWKAQRDLQAMCVDAWRWQSGNP
                     QGYEAG"
     gene            complement(15500..18322)
                     /gene="uvrA"
                     /locus_tag="PKN25_08030"
     CDS             complement(15500..18322)
                     /gene="uvrA"
                     /locus_tag="PKN25_08030"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNV90885.1"
                     /translation="MDQIRIRGARTHNLRNVSLDLPRNRLTVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAIAIEQKATSHNPRSTVGTVT
                     EIHDYLRLLYARAGTPHCPDHPQHALEAQSVAQMVDHVLALPEDTKLMILAPVVAGRK
                     GEQGDLFADLRAQGFVRVRVDGEVMELDALPPLEKGRRHDVEVVIDRVKVRTDLRGRL
                     AESFETALTHADGRALAVEMDSGRAHLFSARFACPVCAFALAELEPRLFSFNNPAGAC
                     PKCDGLGEVEFFDPARVVVHPELSLASGAIRGWDRRNQFYFQMLASLAAHYAFDIETP
                     WQDLPARVREIVLDGSGRTKIAFRYMADNGRMVLKEHPFEGILPNLERRLRETDAVAV
                     REELSKYRATRPCPACHGTRLRSEARHVLVGGRALHEVGHMPLGECRTFFATLQLAGA
                     RAQVAERIVREIADRLGFLIDVGLDYLSLDRSADTLSGGEAQRIRLASQIGSGLTGVM
                     YVLDEPSIGLHQRDNDRLLATLARLRDLGNTVIVVEHDEDAMRSADYLVDMGPGAGEH
                     GGEVVAHGTPDEVIASPRSVTGDYLSGRRSIAIPAQRTRPDPERQLRIVGARGNNLKT
                     VEVSLPVGLLTCITGVSGSGKSTLINDTLARVAAHRLNGAATEPAPHDAIEGLQHFDK
                     LIDVDQSPIGRTPRSNPATYTGLLTPIRELFAGVPEARARGYSPGRFSFNVRGGRCEA
                     CQGDGLVRVEMHFLPDMYVPCDVCHGRRYNRETLEIRYKGRTIDEVLGMTVEQALGFF
                     SAVPNVARKLETLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSRRDTGRTLYILDE
                     PTTGLHFQDIEMLIAVLQRLRAHGNTIIVIEHNLDVIKTADWLIDMGPEGGGGGGMVV
                     CCGTPEEVAAHPASHTGLHLRRVLGG"
     gene            18472..>18866
                     /locus_tag="PKN25_08035"
     CDS             18472..>18866
                     /locus_tag="PKN25_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV90886.1"
                     /translation="MTREERRAGMGLAAIFALRMLGLFLILPVFAVHAATIPGGGDLT
                     LVGLAIGAYGLTQACLQIAYGAASDRFGRKPVIVFGLVLFVLGSVVAALADGIHMIIV
                     GRVLQGAGAISAAVTALAADLTRDQHRTKV"
BASE COUNT         3464 a         5874 c         6316 g         3212 t
ORIGIN      
        1 tcaccccgca cacccacttc accggcgaga tctacgtgct gtcgaaggaa gagggtggtc
       61 gtcacacccc gttcttcaac aactaccgtc cgcagttcta cttccgcacc accgacgtga
      121 ccggttcgat ctcgctgccc gaaggcaccg agatggtcat gccgggcgac aacgtgtcga
      181 tcaccgtcaa gctgatcgcc ccgatcgcca tggaagaagg tctgcgcttc gcgatccgcg
      241 aaggtggccg taccgtcggc gccggcgtcg tcgccaagat catcgagtaa tcgctcgatc
      301 cggggcggct ctcgcggagc cgccccatcg ctctttgcgc tcttttttcg cagtatcagc
      361 tctttaggaa atacatcatg cagaaccaga agatccgcat ccgcctgaaa gccttcgact
      421 atcgcctgat cgatcagtcg gccctcgaga tcgtcgatac cgccaagcgc accggcgccg
      481 tggtccgtgg tcccgtgccg ctgccgaccc gcatcgagcg tttcgacctg ttgcgttcgc
      541 cgcacgtcaa caaggcgtcg cgcgaccaga tggagatccg cactcaccag cgtctgatgg
      601 acatcatcga tccgaccgac aagacggtcg acgcgctgat gaagctggat ctgcccgcgg
      661 gcgtggatgt cgagatcaag ctccagtaag caagccctgc ttgcggaagt ttggaacagg
      721 ccggtataat ccggcctttg tgccttctgg cgcattaatt gtgacccccg gtcaatcgca
      781 accgggattc aggagaagaa aatgagtcta ggccttgttg gacgcaaggt cggcatgact
      841 cgcatcttcg ccgaggatgg caagaccatc ccggtgacgg tgcttgacgt gtcggataat
      901 cgtgtgtccc agatcaagac gatcgacacc gacggctacg ccgcggtgca gattgcgtat
      961 ggcaagcgcc gcgccagccg gatcaccaag ccggtcgcgg gtcatcttgc aaaggcgggc
     1021 gtcgagccct gccgcggcat caaggaattc cgcgtcgagg ccgagcaggc cggcagcatg
     1081 aatgccggcg atgcggtcgg tgcggatctc tttcaggtcg gtcaaaaggt tgacgttacg
     1141 ggtgtgacga tcggcaaggg cttctcgggt ccgatcaagc gtcacaactt ctcgtcgaac
     1201 cgcgcctcgc acggtaactc gatctcgcac aactcccccg gctccatcgg catggcccag
     1261 gatccgggtc gtgtgttccc cggcaagcgc atggcgggcc agtacggcaa cgtccagcgc
     1321 accacgcagg ggctggagat cgttcgtgtg gacgtcgagc gccagctcct tctcgtgaag
     1381 ggtgcggtgc cgggtgccaa gggtggcgac gtcgtcgtcc gtccggcggt caaggcctga
     1441 ggagcgcgca atggaattga aactcttgaa tgatcagggt caggctgcgt cgacgctcga
     1501 agcgtcggac gtgctgttcg gccgtgacta caacgaagcc ctggtgcacc aggtcgtgac
     1561 cgcctacctt gcgaacgccc gttccggcaa ccgcaagcag aagggtcgct ccgagatcgc
     1621 caagtcgacc cgcaagccgt tccgccagaa gggcacgggc aacgcccgtg caggcatggc
     1681 gtcgagcccg ctgtggcgtg gtggcggcaa gatcttcccg agctctcccg acgagaactt
     1741 cagccagaag gtcaaccgca agatgtaccg cgccggtgtg gccgcgatcc tgtcgcagct
     1801 cgctcgcgag gaccgtcttg cggtggtcga gaacttcagc gtcgaagcgc cgaagacccg
     1861 cctgctgtcg cagaagctca aggggatggg cctggattcg gtcctggtca tcaccgacga
     1921 aatcgacgag aacctgttcc tgtcctcgcg caaccttcac caggtcctgg tgctggaagt
     1981 gagcgaggcg gatccggtgt cgctcgtgcg tttccccaag gtgctggtca ccaagggcgc
     2041 gctggcgaag atggaggaag cgtggcaatg agcgcgatca gtcaggaacg tctgctgcag
     2101 gtgctgctcg ccccgcagat ctccgaaaag gcgactttcg tcgccgacaa gaacgagcaa
     2161 gtggtgttca aggttgcgtc cgatgcgacc aaacccgaag tgaaggcggc ggtcgagtcg
     2221 ctcttcaagg tcgaggtgaa gtcggtgcag atcctgaacg tgaagggcaa gtccaagcgc
     2281 tttggcaaga ccatgggtcg ccgcaaggac tggaagaagg ccttcgtgtg cctgaagccc
     2341 ggccaggaaa tcaactttgc ggccggggag tgatagatca tggcaatcgt taaactgaag
     2401 cctacgtctg ccggccgccg tgcccaagtc aaggtggtca accccgatct gtacaagggt
     2461 cgtccggtcg atgcgctgat cgagaagcag accaagaacg ccggccgtaa caactacggt
     2521 cgcgtgacga tgcgccacca gggtggcggc cataagcagc actaccgcct ggtggatttc
     2581 cgtcgcaaca aggacggcat tccggccaag gtcgagcgca tcgagtacga cccgaaccgc
     2641 tccgcgcaca tcgcgctgct gtgctatgcc gatggcgagc gccgctacat catcgcgccg
     2701 cgcggcgtag aggtcggcca gcaactgatc agtggcgcgg aagcgccgat caaggcgggc
     2761 aacgccctgc cgatccgcaa catcccggtc ggctcgacga tccactgcat cgagatggtt
     2821 cccggcaagg gtgcgcaact cgcgcgcgcc gccggcactt cggtgcagct gctcgcccgc
     2881 gaaggctcgt acgcccagct ccgcctgcgc tccggcgaga tccgccgtgt gcacgtcgag
     2941 tgccgcgcca ccatcggtga agtcggtaac ggcgagcatg gcctgcgcaa gatcggcaag
     3001 gctggcgcga accgctggcg cggtatccgt ccgaccgttc gcggtgtggc gatgaacccg
     3061 gtggatcacc cgcatggtgg tggcgaaggc cgcaccggcg aggcgcgcga accggtcagt
     3121 ccgtggggca cgccggccaa gggctatcgg acgcgttcga acaagcgcac cgacggcatg
     3181 atcgtgcagc gtcgtcacaa gcgttaaggg gtaagagatg gcacgttcta tcaagaaagg
     3241 cccgttcatc gacgcgcacc tgctcaagaa ggtcgacgcg gctcggacga gcaacgacaa
     3301 gcgcccgatc aagacctggt cgcgccgctc cacgatcctc cccgatttca tcggcctgac
     3361 gatcgcggtg cataacggca agcagcacat tcctgtattc gtgaccgaga acatggtcgg
     3421 gcacaagctg ggcgaattcg cgctgacgcg gaccttcaag ggtcacgccg ccagcaagaa
     3481 ggccaagcgt taaggggacg acatggaaac caaagctatt ctccgtggcg ttcggctgtc
     3541 ggcccagaag ggccgtctgg tggccgacct ggtccgcggc atgcccgtcg gccgcgcgct
     3601 cgacaccctg gccttctctc ccaagaaggg cgcgaagatc atccgcaagg tgctcgagtc
     3661 ggcgatcgcc aacgccgagc acaacgacgg tgccgatatc gacgcgctga aggtcaagac
     3721 cattcacgtg gaagagggta tgacgctcaa gcgcttcacc gcgcgtgcga agggtcgtgg
     3781 caaccgcatc ctgaagccca cctgccacgt ctacctgacc gttggcgagt aaggagtaaa
     3841 catgggtcag aaaatccatc ctaccgggtt ccgcctcgcc gtcacgcgtg actggggctc
     3901 gcgctggttc gccaacagca aggactacgc cggcatgctg cacgaggacc tcaaggtccg
     3961 cgattacctg aagaagcgcc tcgcgcacgc ttcggtgggt cgcgtggtca tcgagcgtcc
     4021 cgccaagaac gcccgcgtaa ccctgtacag cgcccgcccg ggcgtcgtca ttggcaagaa
     4081 gggcgaggac atcgaggcgc tcaagcgtga tctgcagaag atcgttggcg ttccggtcca
     4141 cgtcaatatc gaggaagtgc gcaagcccga actcgatgca aagctgatcg ccgactcgat
     4201 cgcccagcag ctcgagaagc gcatcatgtt ccgtcgcgcc atgaagcgtg cgatgcagaa
     4261 cgccatgcgt ctcggtgccc agggcatcaa gatcatgagc tccggccgcc tcaacggcgc
     4321 agagatcgcc cgcaccgagt ggtatcgcga agggcgtgtg ccgctgcata ccctgcgcgc
     4381 caacatcgac tacggtgtct ccgaggccaa gacgacctat ggtgtcatcg ggatcaaggt
     4441 ctgggtgttc aagggcgaga tgctggggcg caacgagcag cccgtggctg cggagcccga
     4501 agccgacaac cgtcgcggtc gtcgtggcgc cccgcgtgac ggcgagggcc gtccgggtcg
     4561 tcgtcccgcc cgtcgtggcg aaggccggca agaagaaagc aggagcgaat gatgctgcag
     4621 ccgactagaa ggaagtatcg gaaggagcag aagggccgca acaccggcgt cgccacgcgc
     4681 ggcgccaagg tgtcgttcgg cgagtatggt ctgaaggcca tcggtcgcgg tcggctgact
     4741 gcccgccaga tcgagtccgc ccgtcgtgcg atgacccgtc atatcaagcg tggcggccgc
     4801 atctggatcc ggattttccc ggacaagccg atttcgaaga agccggccga ggtccgcatg
     4861 ggtaacggca agggcaaccc ggaatattgg gttgccgaga tccagccggg caaggtgctg
     4921 tacgagatgg acggggttga cgaggccctg gcgcgcgagg cgtttcgcct tgcggctgcc
     4981 aagctgccga tcgagaccgt gttcgtcacc cgactgattg ggggttgaac atgaaggcaa
     5041 cggaactccg cgccaagagc gccgacgagt tgaacaagga actgatcgag ctgctgaagg
     5101 cccagttttc gctgcgcatg cagcacgcca cgcagcaact cggcaacacg agtcagatcg
     5161 ggaaggtccg ccgcgacatc gcgcgcgtgc gcaccatcct gcgtgagaag gcgggtcagc
     5221 aatgagcgag aacgtcgaga aggttcgccg cgcgctggtt gggcgtgtgg tgagtgacaa
     5281 aatgcagaag acggtgaccg ttctggtcga gcgccgggtc aagcacccgc tctacggcaa
     5341 gatcatcacc cgttcggcga agtaccacgc gcacgtcgag gacggcgtcg cggcccaggg
     5401 cgacctggtc gagatcgagg aatgccgtcc gatctcgcgc accaagacct ggcgtgtggc
     5461 caaggtgctc gagaaggcga cgatcatctg atcggagttg gaagcagcga gcagtaaaca
     5521 aacagcttgg cacccaggtg ccaggctgtt ataattttgc ctctttgctc cggggctcgc
     5581 tcggggcatc ctaccctgac gggttccaag actgtccgca catgcgggat aagttggaga
     5641 tactttcatg attcaaatgc agtccaagct ggacgtggcc gataactccg gcgcgcgctc
     5701 ggtgatgtgc atcaaggtcc tgggtggttc taagcgtcgc tatgccggcg ttggcgacat
     5761 catcaaggtg accgtgaagg atgccgctcc gcgtggtcgt gccaagaaag gcgacgtcta
     5821 caacgcggtc gtcgttcgca ccgccaaggg cgttcgtcgt cccgacggtt cgctgatcaa
     5881 gttcgacagc aatgcggccg tgcttctgaa caacaagctc gagccgatcg gcacccgtat
     5941 cttcgggccg gtcacgcgag agctgcgcac cgagcggttc atgaagatcg tctcgctggc
     6001 gcccgaagtg ctctgaggag tccagagatg aacaagatcc gcaagggtga cgaagttttg
     6061 gtgctgacgg gcaaggatcg tggtcgtcgc ggcaccgtgc tgcgccgcgt cgatgacgag
     6121 cgcgtcgtgg tcgaaggcgt gaaccgggtc aagaagcacg tgcgcccgaa tccgctgaag
     6181 ggtgaagtgg gtggcatcgt cgagaaggag atgccgatcc atgtgtcgaa cgtcgcgctg
     6241 ttcaatcccg ccacccagaa gggtgatcgt gtcgggatca agacgcttga agacggccgc
     6301 aaggttcgtt tcttcaagtc gaacggcgag ctggtgaacg cctaaggagt gacgatggct
     6361 cgcttgcaac aggtttacaa ggacgaagtt gcgccggcgc tgcagcagca gttcggctac
     6421 aagtccgtca tggaagtgcc gcgcatcacc aagatcaccc tcaacatggg tgtcggtgag
     6481 gctgtcggcg acaagaagat tctcgagcac gccattggcg acatgaccaa gatcgcgggt
     6541 cagaagccgg tttcgaccaa ggcgcgcaag tcgatcgcgg gtttcaagat ccgtgacggc
     6601 tatccgatcg gctgcatggt gaccctgcgt ggtccgcgca tgttcgaatt cctcgatcgc
     6661 ctcatcacga tcgcgctgcc gcgcgttcgc gacttccgcg gtatcgctgc caagggtttc
     6721 gacggtcgtg gcaactacaa cctcggcgtg aaagagcaga tcatcttccc cgagatcgag
     6781 tacgacaaga tcgacgcgct gcgcgggatg aacatcagca tcacgacgac ggccaagacc
     6841 gacgaggaag cccgtgctct cctcggtgcg ttcaagttcc cgttcaagaa ttgagggtga
     6901 tatggcgaaa ctcgctctga tcaaccgcga agcgaagcgt gcgatgctgg tggagaagta
     6961 tgcctccaag cgcgcggctc tggtcgcgaa ggtcaaggac accagtctgt ccgaagaaga
     7021 acgcatggct gcccgcctgg ccctgcagca actgccgcgc aatgctaacc cgactcgcca
     7081 gcgcaaccgc tgcgcgctga ccggtcgccc gcgtggtgtc ttccgcaagt tcggtctgtg
     7141 ccgcaacaag ctgcgcgaga tcgctttccg cggcgaagtg cctggcatga ccaaggcgag
     7201 ctggtaagga gaattcgaag atgagtatgt ccgatccgat cgccgacatg ctgacgcgca
     7261 tccgcaacgg ccagcaggcc cagaaggctt cggtgtcgat gccttcgtcc aagctgaagg
     7321 ttgcgatcgc caaggtgttg caggacgagg gttacatcga cggcttcgcc gtccgtgacg
     7381 aggctggcaa ggcttcgctc gacgtcaccc tcaagtatta cgccggtcgt ccggtcatcg
     7441 agcgcctgga gcgcgtgagc cgtcccggcc tgcgcatcta caagggtagc gacgatctgc
     7501 cgcgcgtgat gaacggcctc ggcgtggcca tcgtttccac gccgcgtggc gtgatgaccg
     7561 accgctcggc gcgtgcgaac cgcgtcggtg gcgaagtcat ctgcctcgtc gcctaagggg
     7621 gatacgatgt ctcgtgtagc caagaatccg gtggcgatcc cgcagggcgt ggaagtgatg
     7681 atttccgacg ccgagatcgc agtgaagggg cctctgggta ccctgcgcca gccgctgagc
     7741 ggcgaggtca gcgtggtccg cgaaggtgac gcggtggtgt gcaaggcgcg tgagggcgcc
     7801 gagaacgccc gtgccatgtc cggcaccgtg cgcgctctgg tgaataacat ggtgacgggc
     7861 gtgaccaagg gcttcgagcg caagctcacc ctggtcggcg tgggttatcg cgcgcaggct
     7921 cagggcgaca agctcaacct gtcgctcggt ttctctcacc cggtggttca ccagatgccg
     7981 gaaggcgtga aggtcgagac gccgacccag accgagatcg tgatcagggg ttccgacaag
     8041 cagaaggtcg gccaggtcgc tgccgaggtg cgtgcctacc gcgctcccga gccctataag
     8101 ggcaagggcg tccgttactc ggacgaggtg gtggtgctca aggaaaccaa gaagaagtaa
     8161 ggcggttcgt catgaacaag aaagaaactc gtcttcgccg tgcgcgcaaa actcgcgcca
     8221 agattgcgga gctcaaggcg gttcgcctga cggtgttccg gtccaactgc cacatctacg
     8281 cccagatcat ctccggttgc ggttcccgcg tgctcgcggc ggcttccacc gtggagccgg
     8341 acgtccgtgc ccaggtcgcc aacggcggca acaaggccgc ggccgaagcg gtcggcaagc
     8401 tgattgccga acgcgccaag gctgccggcg tcgagcaggt gtccttcgac cgctcgggct
     8461 tcctgtatca cggccgcgtg aaggcgctgg ccgaggccgc ccgcgaaggc ggtctcaagt
     8521 tctaagcgag gaatcgtatg gccaagcagc aaaccaaacg tgcgcaagcc tccgacgagc
     8581 gcgatgacgg cctgaaggaa aagatggtcg cgatcaatcg cgtgaccaag gtcgtcaagg
     8641 gcggtcgtat cctcggcttc gccgcgctga ccgtggtcgg tgacggcgac ggtgcgatcg
     8701 gcatgggcaa gggcaagtcc cgcgaagtgc cggtggcggt gcagaaggcg atggatgaag
     8761 cgcgtcgcaa gatgcgcaag gtctcgctca agaacggcac ccttcagcat accgtgatgg
     8821 gcaagcatgg cgccgcttcg gtgctcatgc aacccgctcc ggccggtacc ggcatcatcg
     8881 ccggcggtcc gatgcgcgcc gtgttcgaag tcatgggcgt taccgacgtg atctgcaagt
     8941 gcatcggttc ggccaatccg tacaacgtcg tccgtgccac gatcaacggc ctgatggcga
     9001 tcaacacccc ggcggaaatc gctgcgaaac gcggcaagtc cgtcgaagag atcctggggt
     9061 aagagatgtc ggacaagaaa atcaaggtga cgctcgtcaa gagcgtgatc ggcaccaagc
     9121 agtcccaccg cgccaccgtg cgcggcctcg gtctgcgtcg gctcaaccat tcggtcgagc
     9181 tggcggatac ccccgaggtg cgcgggatga tcaacaaggt gtcctacctg gtcaagtgcg
     9241 agggttgaaa tgcgcctgaa tagcatcaag cccggtgagg gttcgaagaa ggaagcgcgc
     9301 cgagtcggcc gtggcatcgg cagcggcctg ggcaagacct gcggccgcgg ccacaagggt
     9361 cagaagtccc gcgccggtgg ctttcacaag gtcggcttcg agggcggtca gatgccgctg
     9421 cagcgccgtc tgccgaagcg tggattcgtg tcgctgacgc gagcccgcaa cgtcgaggta
     9481 cgcctgtccg agctcgacaa gctgccggtc gatgaagtcg atctgctgac gctgaaacag
     9541 gccggggtga tcgccgccga cgcgctgtcg gcgaaggtcg tgctgtccgg ttcgatcagc
     9601 cgcaaggtgg ttctgcgcgg cgtcggcgcc acccagggtg cgaaggcggc gatcgaagcc
     9661 gctggcggct ccgtggcggc cgagtaacaa aaggaaacgg ttgtggcgaa tcctgctgcc
     9721 acgctgggaa gccccggacg gttcggtgat ctcaagcgac gcctgctgtt cctggtgggc
     9781 gcgctgatcg tttatcggat tggtgcgcat atcccggttc cgggaatcga cccgcagggg
     9841 ctggccgagc tgttccagtc ccagcagggt ggcatcctgg gcgtgttcaa cctgttctcg
     9901 ggcggtgcgc tgtcgcgctt caccatcttt gcgctgggca tcatgcccta catctctgcg
     9961 tccatcatca tgcagttgat gaccgtggcg gtgccttcgc tggaggcgct aaagaaggaa
    10021 ggcgaggcgg ggcgacgcaa gatcacgcaa tacacacgct atggaaccct tgtgctcgcg
    10081 cttgcgcagg cgctggggat cgcgatcgcg ctggaaggac aggctggcct ggtgctcgag
    10141 ccgggactga tgttccgctt cgtgacggtg gcgaccttgg tgaccgggac gatgttcctg
    10201 atgtggctgg gtgagcagat caccgagcga ggcatcggca acggcatctc gatgatcatc
    10261 ttcgcaggca tcattgccgg cttgccggct gcgatcggtg gcttgttcga actggtgcgg
    10321 accggtgcca tgcatccctt caccgcgctc ttcatctgca tcctcgtggt gctggtgacg
    10381 gctttcgtcg tgttcgtcga gcgtggccaa cgcaagatcc tggtgaacta cgcgaagcgg
    10441 caagtcggga acaaggtgta tggggggcaa agctcgcatc tgccgttgaa gttgaacatg
    10501 tcgggcgtga tcccgccgat ctttgcatcg agcatcatcc tgtttccggc gacgctgggg
    10561 cagtggttcg gttcctcgga gggcatgtac tggttgcgcg acatcgcatc cacactgtct
    10621 cccggtcagc cgatctatgt gatgttgtat gcggtggcga tcgtgttctt ctgcttcttc
    10681 tacacggcgc tggtgttcaa tgcccgcgaa acggcggaca acctgaagaa gagcggggcc
    10741 tttgtgcccg ggatccgtcc cggcgaccaa accgcgcgct atatcgacaa gattctcatg
    10801 cggctgacgc tggccggggc gatctacatc accctcgtct gcctgctgcc ggagttcctg
    10861 atcctgaagt ggaacgtacc gttctatttc ggcggcacct cgctgctgat cctcgtggtg
    10921 gtcacgatgg acttcatggc ccaggtccag gcgttcgtga tgtcgcacca atacgagagt
    10981 ctgctgaaaa aggcgaattt caagggggcg ggtctgcccg gcaggtgagc gcatgacgaa
    11041 ggaagatgtc atcgagatgc aaggtgaggt cactgagaac cttcccaatg caacgttccg
    11101 cgtcaggctc gaaaacggtc acatggtcct tggacacatc tccggaaaga tgcggatgca
    11161 ctacatccgg atcctccccg gggacaaggt gaccgtgcag ctcaccccct atgacctgac
    11221 caagggccgg atcgttttcc ggaccaagtg aagcttaaag aaacaggagc aagaccatga
    11281 gagtccaggc ctcagtcaag cggctctgcc gcaactgcaa gatcatccgc cgcaagggtg
    11341 tggttcgcgt catttgcagc gatccgcggc acaagcaacg ccagggttga tctactcgac
    11401 cgcaggcatt agaatttaac gtttttcgtt tcggggtata cggatggccc gtattgctgg
    11461 ggtaaacatt cccaatcaca agcatgccga gatcgcgctg accgccatct atgggatcgg
    11521 ccgttcgcgt gctcagaaga tttgcgacgc tgccggcatc gttcgctcgg tgaagatcaa
    11581 ggatctcacc gagtccgaca tggagaagct gcgtgatgaa gtcgcgcgct tcgtggtcga
    11641 aggcgacctg cgtcgtgaag tcacgatgaa catcaagcga ctgatggacc tgggctgcta
    11701 ccgcggcgtc cgtcaccgtc gtggcctgcc gcttcgcggt cagcgcaccc gcaccaatgc
    11761 ccgtacccgc aagggtccgc gcaagccgat cgccggcaag aagtaatagg atccgataat
    11821 ggctaagact ggaaccaagg ttcgcaagaa ggtcaagaag aacgtcgccg aaggtatcgc
    11881 gcacgttcac gcgagcttca acaacaccat catcaccatc accgaccgcc agggcaacgc
    11941 cctgtcgtgg gcgacttcgg gtggcgccgg cttcaagggt tcgcgcaaga gcacgccgtt
    12001 tgccgcgcag gttgcggccg aagctgcggg caaggtggcg caggaatgtg gcatcaagaa
    12061 tctcgaggtt cgcatcaagg gccccggccc gggccgcgaa tcctcggttc gtgcgctcaa
    12121 tgcgctgggg atcaagattt ccagcatcac ggacatcacc cccattccgc acaacggctg
    12181 ccgtccgccg aaaaagcgcc gtatctgaca aggagttgat aagtggctcg taatctcgat
    12241 cccaagtgcc gtcagtgccg tcgcgaaggc gaaaagctgt ttctgaaggg cgaaaagtgc
    12301 tttaccgaca agtgctccat cgagcgtcgc gcctacgcgc ccggccagca tggccagcgc
    12361 tccggccagc gcctgtcggg ctacggcgtc cagctgcgtg agaagcagaa gatccgccgc
    12421 atctacggcg tgctcgagcg ccagttccgc aaggtgtatg ccgaagccga ccgccgccgc
    12481 ggccagaccg gcgagaatct tctgcagctg ctcgaaggtc gcctggatgc cgtcgcgtat
    12541 cgcatgggct tcggtgcctc gcgtgcagag gcgcgccagg tggtgcgtca caacggcgtg
    12601 ctggtcaatg gcaagcgcgt caacattccg tcgtatgtgg ttcgtccggg cgacgtggtg
    12661 gagctgactg agcgtgcgcg tggtcatctg cgcgtcaagg ccgccctcga ggccgccgag
    12721 tcgcgtggct tccccgagtg gctcgaggtg gacgcgaagg ccggcaaggg cacgttcaag
    12781 gcctatccgg tgcgcagcga actgcccccg acgatcaacg aaggcctggt cgtggaactg
    12841 tactcccgct gagcgggggg ctgatcctct tctagttccg aggaaatgct gatgcaaagc
    12901 aatgcactgc tgaaaccgcg catcatcgac gtccagagca cctcgcccgt tcaggcgcgg
    12961 gtgacgatgg agccgttcga gcgcggtttc ggtcacacgc tggggaatgc gcttcggcgc
    13021 atcctcctgt cgtcgcttcc cggccatgcg ccgaccgaag tgacgatcga aggggtgctt
    13081 cacgagtact ccacgctcga cggcgtccgc gaggacatcg tcgatctgct gctcaacctg
    13141 aagggcgtgg tgttcaagct caacgggcgc agcgaggtca ccgtccgtct cgccaaggca
    13201 ggcgagggtg tggtgactgc ggccgacatc gaggtcggtc acgacgtcga gatcatcaat
    13261 cccgaccacg tgatcgccca cctcgcgccg ggcggcaagc tcgacatgca gatcaaggtc
    13321 gaggaaggcc gcggctacgt gccgggcaat tcgcgtcccg agaacgcgga gagcaagtcc
    13381 atcggtcgcg tcgtgctcga tgcctccttc ggtcccgtgc gtcgcgtcag ctaccttgtc
    13441 gagagcgctc gcgtcgagca gcgtaccgac ctggatcgcc tgatcatcga catcgagacc
    13501 aacggtgcgg tcgacccgga agaggcgatt cgctacgccg cccgggttct catggaccag
    13561 ctttccgtgt tcgccgacct cgaggggacg gcaccggagc gcgtcgaggc ggcttcgccg
    13621 accatcgacc cggtgctgct gcgcccggtc gacgatctgg agctcacggt gcgctcggcc
    13681 aactgcctga aggccgagaa catctactac atcggcgacc tgatccagcg taccgagacc
    13741 gagctgctga agaccccgaa cctgggccgc aagtcgctca acgagatcaa ggaagtgttg
    13801 gcctcccgcg ggctgacgct tggcatgaaa ctggaaaact ggccgccggc cgggcttgaa
    13861 aagctcggtt gagtcgccgg gcaagtgagg aattgaaatg cgtcaccgta atggtcttcg
    13921 caagctgaac cgtaccagca gccaccgcca ggcgatgttc cgcaacatgg ccaactcgct
    13981 gctgcgtcat gagctgatca agacgaccct tccgaaggcg aaggaacttc gccgcgtggt
    14041 cgagccgatg atcaccctgg gcaagaagcc cagcctctcc aaccgccgcc tggccttcaa
    14101 ccgcctgcgc gatcgcgaca tggtcgtcaa gctcttcgac gagctgggtc cgcgctacgc
    14161 cacccgcaac ggcggctacc tgcgcatcct gaagtgtggt ttccgcgatg gcgacaacgc
    14221 cccgatggcc tacgtcgagt tgctcgaccg tccggacgtg gaagcggtgg aagtcgagga
    14281 agccgaggcc gcgtgaaacg cgcgactcgt acgaagaacc gggctcaggc ccggtttttt
    14341 ttcgcctgtg cgtgctaggg tcgagcgcag cgcgcccgcg ggcggacagg acaggaggat
    14401 ggaacgatgg cagtgacctt ggtgaccggc ggtgcgggct acatcggctc gcatacctgc
    14461 gtcgagttgc tcggcgccgg gcaggacgtg gtggtggtgg acgacctctc caacgcgtcc
    14521 ttcgaggcgg tgcggcggat cgaagagctc ggaggcaagc cgctgaccgg cttcgtggag
    14581 ggcgacgtgc gggaccatgg cgtgctcgcg cgggtcttcg gcgagcacga ggtggcggcg
    14641 gtgatccact ttgccgccaa gaaggccgtg ggcgagtcgg tgtcccaacc gctggcctat
    14701 tacgacaaca acctgcatgg actgctgtcg gtgctgcagg ccatggacgc agcgggatgc
    14761 cggcgcatgg tgttcagttc gtcggcgacg gtgtatggcg acccggcctc ggtgccgatc
    14821 cgcgaggatt tcccgactac ggcgaccaat ccttacggct ggaccaagct catgggcgag
    14881 cagatcctgc gtgatctcgc cgcctcggat ccacgctgga acgtggtgct gctgcgttac
    14941 ttcaaccctg tcggggcaca cgcgagcggt cggatcggcg aggatcccga cggcctgccc
    15001 aacaacctga tgcctttcgt cagtcaggtc gcggtcggcc gcctgccgcg cttgcgcgtt
    15061 ttcggcgacg actacgcgac gccggacgga accggggtgc gcgactacat ccacgtggtc
    15121 gacctggcgc tcggccattt gcgcgcgctc gagcgcatcg acgaactccc cggcgtgacg
    15181 acgctcaacc tcggcaccgg gcgcggctac agcgtgctgg agatggtgcg cgccttcgag
    15241 gcggcgagcg ggcgaccggt cccctacgac atcgtcgcgc gccgtccggg cgatatcgcc
    15301 gcgtgctggg cggaccctgc gcgcgccgag caggtgctcg gctggaaggc gcaacgagac
    15361 ctgcaggcga tgtgcgtgga tgcgtggcgc tggcagagcg gcaacccgca gggctacgaa
    15421 gccggttgag taactcgcgg cgcaccgctc gggcgccgca aaggggcgcg gaagcccggt
    15481 ggggtggatc ggccggggct cagcccccga gcaccctgcg caggtgcagg ccggtatgac
    15541 tcgccggatg cgcggcgacc tcttcgggcg tgccgcagca caccaccatg ccgccaccgc
    15601 cgccgccctc cgggcccatg tcgatcagcc agtcggcggt cttgatcacg tcgagattgt
    15661 gctcgatcac gatgatcgtg ttgccgtgcg cgcgcaagcg ctgcagcact gcgatcagca
    15721 tctcgatatc ctggaaatgc aggccggtgg tcggctcgtc gaggatgtac agggtgcgcc
    15781 cggtatcgcg gcgcgacagc tcgagcgcga gcttgacgcg ctgcgcctcg ccgcccgaca
    15841 gggtggtggc gctctggccg aggcggatgt agcccaggcc gacgtccatc agcgtctcca
    15901 gtttgcgcgc cacgttcggc accgcggaga agaaacccag ggcctgctcg acggtcatcc
    15961 ccagcacctc gtcgatcgtc cgccccttgt agcggatctc gagggtctcg cggttgtagc
    16021 ggcgaccgtg gcagacgtcg cagggcacgt acatgtcggg caggaagtgc atctccaccc
    16081 tgaccaggcc gtcgccctgg caggcctcgc aacgcccgcc gcgcacgttg aacgagaagc
    16141 gccccggact gtagccgcgc gcgcgggcct cgggcacgcc ggcgaagagc tcgcggatcg
    16201 gggtcagcag gccggtgtag gtcgccgggt tggagcgtgg ggtgcgcccg atcggcgact
    16261 gatcgacgtc gatcagcttg tcgaagtgct gcaggccctc gatcgcatcg tgtggcgcgg
    16321 gctcggtggc ggcaccgttg agccggtggg ccgcgacgcg tgccagggtg tcgttgatca
    16381 gcgtcgactt tcccgagccc gacaccccgg tgatgcaggt gaggagcccc accggcagcg
    16441 acacctccac cgtcttcagg ttgttgccgc gcgcgccgac gatgcgcagc tggcgctccg
    16501 ggtcggggcg cgtgcgctgc gccgggattg caatgctgcg ccggccggag aggtagtcgc
    16561 ccgtgaccga acgcggtgag gcgatgacct cgtccggcgt gccgtgcgcc accacctcgc
    16621 caccgtgctc gcccgcgccc gggcccatgt ccaccagata gtcggcgctg cgcatggcgt
    16681 cctcgtcgtg ttcgaccacg atcaccgtgt tgccgaggtc gcgcaggcgc gcgagcgtgg
    16741 ccagcaggcg gtcgttgtcg cgctggtgca ggccgatcga aggctcgtcg agcacgtaca
    16801 tcaccccggt cagacccgag ccgatctggc tcgccaggcg gatgcgctgc gcttcgccgc
    16861 ccgagagggt gtcggcggag cggtcgagcg agaggtagtc gaggcccacg tcgatcagga
    16921 agccgaggcg gtcggcgatc tcgcgcacga tgcgctcggc cacctgggcg cgcgcgccgg
    16981 cgagttgcag cgtggcgaag aaagtgcggc actcgccgag cggcatgtgg ccgacctcgt
    17041 gcagcgcccg cccgccgacg agcacgtggc gcgcctcgct gcgcaggcgg gtgccgtggc
    17101 aagcggggca ggggcgggtg gcgcggtact tcgacagctc ctctcgcacc gcgaccgcgt
    17161 cggtctcgcg caggcggcgc tccaggttgg ggaggatgcc ctcgaaggga tgctccttga
    17221 gcaccatgcg gccgttgtcg gccatgtagc gaaaggcgat cttcgtccgc cccgatccat
    17281 cgagcacgat ctcgcgcacg cgcgccggca gatcctgcca gggcgtctcg atgtcgaagg
    17341 cgtagtgcgc cgcgaggctc gccagcatct ggaaatagaa ctggttgcgc cggtcccagc
    17401 cgcggatcgc gcccgaggcc agcgacagct cgggatggac cacgacgcgt gcgggatcga
    17461 agaactcgac ctcgcccagc ccgtcgcact tcgggcaggc gcccgccggg ttgttgaagg
    17521 agaacaggcg cggctcgagc tcggcgagcg cgaaggcgca caccgggcag gcgaagcgcg
    17581 cggagaacag gtgtgcgcga cccgagtcca tctccaccgc gagcgcgcgc ccgtcggcgt
    17641 gggtcagcgc ggtctcgaag gactccgcga ggcggccgcg cagatcggtg cgcaccttga
    17701 cgcggtcgat cacgacctcg acgtcgtggc ggcgcccctt ctcgagcggg ggcagggcgt
    17761 cgagctccat cacctcgccg tcgacgcgca cgcgcacgaa accctgcgcg cgcagatcgg
    17821 cgaacaggtc gccctgctcg cccttgcgcc cggccaccac cggggcgagg atcatcagct
    17881 tcgtgtcctc gggcagtgcc agcacgtggt ccaccatctg cgccacgctc tgcgcctcga
    17941 gcgcgtgctg cggatggtca gggcagtgcg gcgtgccggc acgcgcgtag agcaggcgca
    18001 ggtagtcgtg gatctcggtg accgtgccga cggtggagcg cgggttgtgg ctggtcgcct
    18061 tctgctcgat cgcgatcgcc ggcgacaagc cctcgatcag atcgacgtcg ggcttttcca
    18121 tcagctgcag gaactggcgg gcgtaggccg acagcgactc gacgtagcgg cgctggccct
    18181 cggcgtagag ggtgtcgaag gcgagcgagg acttgcccga gcccgacagg ccggtgatca
    18241 cggtcagccg gttgcgcggc aggtcgaggc tgacgttcct gaggttgtgg gtgcgggcac
    18301 cgcggatgcg gatctggtcc atggctgcga gcagggaaag gcgaactgcc caatatacgc
    18361 cgggaagccg ccgcaaggcc aaggcggtcc gcgcagcgcg gggcgggatg gtagaatcgc
    18421 ggtctttctc gaagccggtt cctccgtatg tccgttcccg cacgcgaccc catgacgcgc
    18481 gaagaacgcc gcgccggcat gggcctggcg gcgatcttcg cgctgcgcat gctcggcctg
    18541 ttcctgatcc tgccggtgtt cgcggtgcac gccgccacca tccccggggg cggcgacctc
    18601 accttggtgg gcctggcgat cggcgcctac ggcctcaccc aggcctgcct gcagatcgcc
    18661 tacggcgccg cctccgaccg cttcggccgc aagccggtga tcgtcttcgg cctggtgctg
    18721 ttcgtgctcg gcagcgtggt cgcggcgctc gccgacggca tccacatgat catcgtcggc
    18781 cgcgtgctgc agggtgcggg ggcgatctcc gccgcggtca ccgcgctcgc cgccgacctc
    18841 acccgcgacc agcaccgcac caaggt
//
LOCUS       DAOQBS010000055        17972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2233_length_17972_cov_14.888374, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000055 DAOQBS010000000
VERSION     DAOQBS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 17972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17972
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2233_length_17972_cov_14.888374"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /gene="asnB"
                     /locus_tag="PKN25_08040"
     CDS             complement(<1..1040)
                     /gene="asnB"
                     /locus_tag="PKN25_08040"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNV90887.1"
                     /translation="MCGIAGLFDTRGKRDFERALMQRMNDVQHHRGPDEGGYHFEPGV
                     ALAHRRLSIIDLATGQQPLFNEDGSVAVVFNGEIYNFQELVPELEALGHVFHTRSDTE
                     VIVHAWEAWGEDCVQRFRGMFAFALHDRNRDTFFMARDRLAVKPMFYALLPDGTLAFG
                     SELKVLMQHPALDRRIDPLAVEEYFALGYVAEPRTIFLGARKLGPGESLCIRRGQPIP
                     APKQYWDVRFTLDNDIGLADATAELTERLRESVRLRMIAEVPLGAFLSGGVDSSAVVA
                     TMAGLSDDPVNTCSIAFDDPKYNESAFAQMVADRYRTRHFVETVKSDDFDLIDTLAAL
                     YDEPYADSSAIPT"
     gene            complement(1056..2222)
                     /locus_tag="PKN25_08045"
     CDS             complement(1056..2222)
                     /locus_tag="PKN25_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNV90888.1"
                     /translation="MKDVARPLVLHLVYRFDVGGLENGVVNLINHLPAERFRHAVVAL
                     TECTEGFSRRIERADVRFVSLHKPPGHGLKLYPRLYRLFRALRPAIVHTRNLAALEAV
                     VPARLAGVPATVHGEHGWDVSDPGGTRRKFQWLRRAYSPFVNRYVALSGHIESYLVER
                     VGISPARVARICNGVDARRFHPRLSAQARSLAGLPEGFATPGTVIVGTVGRQQAVKDP
                     LALVRAFALARTHGEVGGRLRLLLAGDGPLRATLEAEIRSSGLEDAVWLAGERADVPA
                     VMRALDVFALPSRAEGISNTVLEAMASGLPVVATAVGGNAELVVPGETGALVAAEDPR
                     GMADALLCYAADAALRQKHGLAARRRVEAEFSIERMVARYAELYESLLRPQGAA"
     gene            complement(2219..3508)
                     /locus_tag="PKN25_08050"
     CDS             complement(2219..3508)
                     /locus_tag="PKN25_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNV90889.1"
                     /translation="MQDSRPPLLYLTHRIPYPPNKGDKLRSFNILRQLARTHRVWLGS
                     FVDHADDRRHIPALRQWCEQTCVIPIEPGIRRFASLRGLLRGEALSLPYYRSPRLARW
                     VDQVVAENGIRSAVAFSGPMAQYLDVPGLSRRVVDFCDVDSAKWTQYAADRRWPMSWL
                     YRREGERLLDFERRAAAGVEASLFVTEAEAEVFRRAAPEVGDRVGVMQNGVDAEYFAP
                     ANAGASPYPAGAPVIAFSGAMDYWPNVDAVCWFATELLPAIRRAMPGVRFWIVGMNPA
                     PAVQALAGEDVVVTGTVPDVRPYIAHADLVVAPLRIARGIQNKVLEAMAMARPVVLSA
                     APAVGLAARDGVECSIADDGDELCRRVVELLGDPSRRAAIGAAARDCVLRAYSWSAHL
                     RTLDWLLDAQGTDAGLAQAVQAREGAAPNAAALVRTP"
     gene            complement(3513..4562)
                     /locus_tag="PKN25_08055"
     CDS             complement(3513..4562)
                     /locus_tag="PKN25_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HNV90890.1"
                     /translation="MDRLPIVVNLLKAEDALRWDEFVRRCPQATFFHLSAWRELMEEV
                     FDHRTFYLYAERAGDIVGVLPLAEVRSRLFGHALVSLPFCVYGGIAAAEDAGPETLYA
                     LESKAETLAQALGVQHLEFRNLKPRHEDWPRQELYVTFRKEILPEVEANLLAIPRKQR
                     AMVRKGIKNGLVGEVDAGVDRFFALYADNVLRHGTPALPRKFFEQLKERFGEACEVLT
                     VTSPEGKPLSSVLSFYFRDEVLPYYAGDDLAARELAANDFKYWELMRRACERGCTVFD
                     YGRSKVGTGPYAFKKNWGFEPQPLSYEFCLYKRDSIPQNNPMNPKYRAFIALWKRLPI
                     GVANRLGPHIVRNLG"
     gene            complement(4588..5427)
                     /locus_tag="PKN25_08060"
     CDS             complement(4588..5427)
                     /locus_tag="PKN25_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HNV90891.1"
                     /translation="MAARITNAFTCDVEDYFQVSALAPHFPREQWDSVPCRVERNVDR
                     ILALLDGHGARGTFFTLGWLAERYPGLVRRIAAAGHEVASHGYAHQRASDLTPEAFTA
                     DIRLAKVILEDITGSAVTGYRAPSFSIGEANLWAHDCIAEAGYRYSSSVYPVRHDHYG
                     IPDAPRFPWRLPNGLVEVPITTVHMLGRNWPAGGGGFFRLLPYALSRWQIARVNAADR
                     RPAIFYFHPWEIDPEQPRVTAATAKTRFRHYINLQHTEARLDRLLSDFSWGRADEVFR
                     DAA"
     gene            complement(5429..6595)
                     /gene="wecB"
                     /locus_tag="PKN25_08065"
     CDS             complement(5429..6595)
                     /gene="wecB"
                     /locus_tag="PKN25_08065"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNV90892.1"
                     /translation="MSAHRLSAAPVICIVGARPNYMKVAPIIRAFAAHQPPIPTLLVH
                     TGQHYDPAMKDRLFADLDLPEPDVNLAVGSGSHAVQTAEVMKRFEPVIDEHGARAVLV
                     VGDVNSTLACALVASKRHIPVVHVESGLRSNDRRMPEEINRILTDQISDVLYTTERSA
                     HDNLVREGIPAERAVFVGNVMIDSLRASLPRAVPADVLLAAAGLDASRIAGGYGVVTL
                     HRPSNVDSADTLGPIIDALRTVSEALPLVVALHPRTRNNLERFGMLDRLAGSGFLILP
                     PQGYLEMLGLMSGARMVLTDSGGIQEETTALGVPCVTIRENTERPITVEQGTNTLVGI
                     RPEAIVAAAREILATGGKAGRVPEYWDGRCAERIAAHLHDWLVEHETVHAAPGG"
     gene            complement(6592..7686)
                     /locus_tag="PKN25_08070"
     CDS             complement(6592..7686)
                     /locus_tag="PKN25_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated ATPase"
                     /protein_id="HNV90893.1"
                     /translation="MYETFFKLSGKPFQLNPDPAFFYGSRGHRRAMAYLEYGLHQSEG
                     FIVITGEVGAGKTTIVRSLLEQLDPAKVVAATIVSTQIDASDMLRMVAGAFGVPSRNL
                     DKAGLLLAIETYLVSLAAADKRALLIIDEAQNLSAAAVEELRMLSNFQLEDHALLQSF
                     LVGQPEFREMMQRAEMQQLRQRVIASYHLGPLDLQETRGYIEHRLRHVGWNEDPRFEP
                     GSFNAIYGHTGGIPRTINTLCDRLLLAAYLGESHVVTEAQVREVIAELKEEFAPPRTR
                     PVAGMGRAVLRDAEPAAAMPPVEALAVERLRVSPELADQVAGMAGSYDLQRIEARLAG
                     LEQSVSATLAVLNQLLQAVRRDAATEEEGR"
     gene            complement(7698..9143)
                     /locus_tag="PKN25_08075"
     CDS             complement(7698..9143)
                     /locus_tag="PKN25_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HNV90894.1"
                     /translation="MLALLCPATGAVAQSTRVTPTFDTELTWTDNAGLDREGGRDWLL
                     EVSPGVSVSRSAGRLNGSLSARLRNLVHSSASEDDSTYLSFDGRGAYEALEDLLFLDF
                     SGGITRNNYSAFSGRAPGDPFGFDKDNEVRYWSITPRLESDLRFGEAARGSVSYSSSG
                     VRSNSSGLGDQSADTVEASLADPGGLRFFGWGLAYARTESDGGTAATGQRGEESVRGT
                     LYANVSPQLRLRGIVGHESNDYADGRKDSSGIVGAGVDWYPTDRTSLAATVEDRVFGR
                     GYDLALNHRMRRAVFFLGATRDVSSLAQTLGTVVLADRDCFMRVRDIADPIEREQALN
                     ECLTFGVLRSNAAYVERAFRGGVSLLGVRNTLSLSASRSDRSRIGSTTGLLPEDDLRD
                     TERVRITTTTLAWSHTLSGTSSLYATLARSDSESAADADLALRRLTGTVGVDRTLGPR
                     TRAGLRYRYEKTEGESDYTTNSVTAILGMRF"
     gene            complement(9178..10122)
                     /locus_tag="PKN25_08080"
     CDS             complement(9178..10122)
                     /locus_tag="PKN25_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HNV90895.1"
                     /translation="MSIIEKAVKRLDQLKSAAGEGAVESAPVPEALVLPEDKPLAPKV
                     ESNAPVVATPAPVRAQPKSVHIDLARLAARGLVTPDQPRSAIAEEFRLIKRPLLANAV
                     LEGPAAVDNGNLIMISSALPGEGKTFNAINLAISMAMELDYTVLLVDADVSRPSVLRE
                     FDLPPQPGLMDVLAGEVEDLSEVLLKTNIDKLSILPAGMPHQRATELLASDGMTQLLE
                     QMAHRYADRIIVFDSPPLLVTTESRVLATHMGQVVLVVEAERTTHAHVRQALAAIEPC
                     PIRMMVLNKSRDRSVGGYYGYGYGYGYGKAPEASSEAA"
     gene            complement(10136..11680)
                     /locus_tag="PKN25_08085"
     CDS             complement(10136..11680)
                     /locus_tag="PKN25_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNV90896.1"
                     /translation="MEELVTQIIGYLRGMWRFRWWGLALAWVAGIAGSLAIYFMPDHY
                     ESSARIHVDTQSVLRPLMSGLAVQPNISQQIDLLSRTLISRPNVEKLITMADLDLTVQ
                     NAQQREALITSVTSRLRIQSGRGDNLFTLSYEDVEPHRAQRVVQSLLSLFVESGLGGK
                     RQDTDAARRFIEDQIRSYEQKLTDAENRLKDFRLRNMALLGTGARDYVTQIAETNEQL
                     REARLELREAENSRDALRQQLSAAPSDAGIAPPPMVATPEIDARIDLLKRNLDEMLQR
                     YTEMHPDVVGARRVIEDLEKQKKAQLAELSASMAGSTFGAPGAMSAGVEQTRLAVAQA
                     EARVASLRARVAEFESRLASLSEDAKRIPELETEMAQLNRDYSVHKSNYDQLVSRRES
                     ANIAAEMSTQSGIADFRIIDPPTLPTKPSAPNRLLLLPLAALVGLGAGFALTFLISQL
                     RPAFSDARQLREITGLPVLGTVSMLTTSERRRRRRNGLFAFGSGLVAYAGVFAAVTVA
                     IVFLQS"
     gene            complement(11751..12380)
                     /locus_tag="PKN25_08090"
     CDS             complement(11751..12380)
                     /locus_tag="PKN25_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HNV90897.1"
                     /translation="MFRSVKSASSAVLHFLVLAVAAGLLGGCASSFPPAPASAADADY
                     NYVIGPGDSVNIVVWRNPELSTSVPVRPDGKITAPLVEDLPAMGKDSSTLARDIEKQL
                     AKFIRDPVVTVIVGGFVGPYSEQIRVIGEAANPQTLAYKQKMTLLDVMIAVGGLTDFA
                     DGNGASILRTAEGNKQYSVRIRDLVKRGDVSANVEMRPGDVLIIPQSWF"
     gene            12631..13428
                     /locus_tag="PKN25_08095"
     CDS             12631..13428
                     /locus_tag="PKN25_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90898.1"
                     /translation="MRQIHSSRGTNCPPPVRDLAYLALSGAGFQSPETSPHQWFDENT
                     AALRGLSFERSGFQVCIANGSEALKRQTAKLIERMYSARGLFPYGMHANIDPRDITVV
                     ALLDGEAVATATIRVDHGGGLLADTLYSKEIDHVRATGGRVCEITQLAIDPERGSHEA
                     LAGVMQAVFVITQATRRVTDIFAEVNPRHAGFHQRVFGFRRVGVEKICPRVNAPAVLL
                     HLPLSEFEERVARHERLMHMNGDRVHRLLPDPVQSRQLIQAMTGKAA"
     gene            complement(13444..14871)
                     /locus_tag="PKN25_08100"
     CDS             complement(13444..14871)
                     /locus_tag="PKN25_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90899.1"
                     /translation="MPLSKKLPCLTVLALVLGALSVPATAAGPQLAPKPVSVEVEEGE
                     PAAEEPVTDADAGEADAELDALLDVGAPASGAEGADGLKWNGYTELGAAYTYRDPERW
                     SRLRARGELGVAGQLAPRARWKLSARAEADGAFDLEDEHYPAAVRRDRRTDFVLREAY
                     VDLGHGDWEFRAGRQQIVWGEMVGFFFADVVSARDMRDFLQPELEGMRIGQWALRAEH
                     FGAETHLEFLWVPKPSFDEIGEPGDDFFVFPWLPAGTVLDEDRPGKDLDGSNWGARVS
                     RLVDGWDLSAFYYRSYDVSPTLYALNQGVARLRHDRIGQLGGTFSKDLGSFVLKGEAV
                     HTHGRSLNTFSSGPGLSIGLLPTDMVDYALGVDVPAGDWRFNVQYYGRWLEEHVPALM
                     ADRHEQGVTLQVVHGAGTNLEAEVLALSSINRSDHLIRPKLTWKFAPAWRLVGGVDVF
                     GGNGRGFFSRYDRNDRVYLELRHMF"
     gene            complement(14883..15632)
                     /locus_tag="PKN25_08105"
     CDS             complement(14883..15632)
                     /locus_tag="PKN25_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90900.1"
                     /translation="MAIVQIEHVSKSFRLGEQMVLALDDVSLEIEPGVFLAIAGPSGS
                     GKSTLLNIIGCIDTPSSGRVVVDGHDVSNRTPDELADLRARTIGFIFQTFNLLPVLSA
                     AENVEYPLLQMPELTKKERAERVRHYLGVVGLKKYAEHRPNQLSGGQRQRVAIARALA
                     THSKIVLADEPTANLDSKTGTSILKLMRDINRQSGTTFVFSTHDRKVMNMADRLVRIA
                     DGQIIALGMKADDRWVFVDGRTPRDEDIREI"
     gene            15676..16479
                     /locus_tag="PKN25_08110"
     CDS             15676..16479
                     /locus_tag="PKN25_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNV90901.1"
                     /translation="MTPSLRSRRRFVACLAGSLLLPTLAHATDAPAPAEPAPPEPVDA
                     VAIDLVQRADAIRFPRESFQTEVVVRNYTDGAEGDSRTYRILSRGNENTIVLTMEPAT
                     ERGQALLMRARDLWIFMPSVSQPVRLSLAQRLTGQVANGDLARANLAGDYTPTIAGRE
                     TIDGQPVVVLDLKAVDRGVTYARVRYWVGERDQRPLKAEFYALSGRLLKTARYEEFRQ
                     MAGAIRPTRLVMEDALKKGEISVLTYENMNVRDLPERMFTREYLRRLQQ"
     gene            complement(16504..17913)
                     /locus_tag="PKN25_08115"
     CDS             complement(16504..17913)
                     /locus_tag="PKN25_08115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV90902.1"
                     /translation="MNPTILHHGARDGVTGSCHQLFVEGRNSLLVDCGLFQGAETAPD
                     GRSAADRLEIDFPLEGVGALVLTHVHIDHCGRLPWLLAAGFKGPILCSEPSARLLPTV
                     LADAFAVGVSRDRALIERYLGKVEARIRALPYGKWHLVYRSPDASCRIRLQRAGHILG
                     SAYVECELSGAAWPQPLRVVFSGDLGAPHAPLLPAPRPPWRADVLVLETTYGDRVHEN
                     RRDRRARLQAVVEHALEDGGTVIVPAFSIGRTQELLYEFEDILHRQRMRPGPHAAAWG
                     GLRIFLDSPLAQRFTALYRELQPFWDAEARARVRAGRQPLSYDQLVAIDSHEDHLANV
                     RRLARSREPAVVIAASGMCAGGRVVNYLREMLGDPRHDVLFVGYQARGTPGHAIQAYG
                     PRGGYVELDGERVDIRAGIHTLSGYSAHADRDDLTRFVTRMRHLPGEVRLVHGEDGVR
                     AEFARHLQEATGGHVRVVV"
BASE COUNT         2876 a         6074 c         6274 g         2748 t
ORIGIN      
        1 gtggggatgg ccgagctgtc ggcgtagggc tcgtcgtaga gcgcggccag ggtgtcgatg
       61 aggtcgaagt cgtcggactt gacggtctcg acgaagtggc gggtgcggta gcggtcggcg
      121 accatctgcg cgaacgccga ctcgttgtac ttgggatcgt cgaaggcgat cgagcaggtg
      181 ttgaccggat cgtccgagag ccccgccatg gtcgccacca ccgcgctcga atcgacccct
      241 ccggagagga aggcacccag cggcacctcg gcgatcatgc gcagccgcac cgattcgcgc
      301 aggcgctcgg tcagctcggc ggtggcgtcg gcgaggccga tgtcgttgtc gagggtgaag
      361 cgcacgtccc agtactgctt cggcgcgggg atcggctggc cgcggcggat gcacagcgat
      421 tcgccggggc cgagcttcct ggcgccgagg aagatggtgc gcggctcggc gacgtaaccg
      481 agggcgaagt actcctcgac cgcgagcggg tcgatgcggc ggtcgagcgc cgggtgctgc
      541 atcagcacct tgagttcgga accgaaggcg agcgtgccgt cgggcagcag cgcgtagaac
      601 atcggcttga ccgcgaggcg gtcgcgcgcc atgaagaagg tgtcgcggtt gcggtcgtgc
      661 agcgcgaacg cgaacatgcc gcggaagcgc tgcacgcagt cctcgcccca ggcctcccag
      721 gcgtgcacga tgacctccgt gtcgctgcgg gtgtggaaga cgtgaccgag cgcctcgagc
      781 tcgggtacga gctcctggaa gttgtagatc tcgccgttga acaccaccgc caccgagccg
      841 tcctcgttga acagcggttg ctggccggtg gcaaggtcga tgatggacag gcggcggtgg
      901 gcgagcgcga cgcccggctc gaagtggtag ccgccctcgt ccgggccgcg atggtgctgg
      961 acgtcgttca tgcgctgcat cagggcgcgc tcgaagtcgc gtttgccacg ggtgtcgaag
     1021 aggccggcaa tgccgcacat gatcgcgtgt cctcgtcagg ctgcgccctg cgggcgcaga
     1081 agggattcgt agagctcggc gtagcgggcc accatgcgct cgatgctgaa ctccgcctcg
     1141 acgcgccggc gcgcagccag gccatgtttt tgccgcagcg cagcatccgc agcgtagcac
     1201 agcaacgcgt cggccatgcc gcgcgggtct tcggccgcca cgagcgcgcc ggtctcgccg
     1261 ggcacgacca gttccgcatt gccgccgacc gcggtggcga cgaccggcag cccgctcgcc
     1321 atcgcctcga ggacggtgtt ggagatgccc tcggcgcgcg acggcagcgc gaacacgtcg
     1381 agcgcgcgca tcaccgcggg cacgtcggca cgctcgccgg ccagccagac ggcgtcttcc
     1441 agcccgctgc tgcggatctc ggcctcgagc gtggcgcgca gcggcccgtc gcccgcgagc
     1501 agcaggcgca gcctgccccc gacctcgccg tgtgtgcgcg cgagcgcgaa ggcgcgcacc
     1561 agggcgagcg gatccttgac cgcctgctgc cgcccgaccg tgccgacgat gaccgtgccg
     1621 ggggtggcga agccttccgg cagaccggcg aggctgcgcg cctgcgccga caggcggggg
     1681 tggaagcgcc gcgcgtccac gccgttgcag atgcgcgcga cccgcgcggg ggaaatgccc
     1741 acgcgctcga cgagatagga ctcgatgtgg cccgagagcg ccacgtagcg gttcacgaag
     1801 gggctgtagg cgcgccgcag ccactggaac ttgcgccgcg tgccgccggg gtcggagacg
     1861 tcccagccgt gctcgccatg caccgtcgcc ggcacgccgg cgagccgcgc cggcaccacc
     1921 gcctcgagcg cggcgaggtt gcgcgtgtgc acgatcgcag gacgcagcgc gcggaacagc
     1981 cggtacaggc gcggatagag cttgaggccg tggccgggcg gcttgtgcag cgacacgaag
     2041 cgcacgtccg cgcgctcgat gcggcgcgag aagccttcgg tgcattcggt gagcgccacc
     2101 accgcatgac ggaagcgctc ggcagggagg tggttgatca ggttcaccac cccgttctcc
     2161 aggccgccga cgtcgaagcg gtacacgagg tgcagcacca gcggccgggc gacgtccttc
     2221 atggtgtccg cacgagcgcc gccgcgttcg gcgcggcgcc ctcgcgggcc tgcaccgcct
     2281 gcgcgaggcc tgcatccgtg ccctgcgcat cgagcagcca gtccagggtg cgcaggtgcg
     2341 ccgaccagct gtaggcgcgc agcacgcagt cccgcgccgc cgcgccgatc gcggcgcgac
     2401 gcgacggatc cccaagcagc tcgaccacgc gccggcacag ctcgtcgcca tcgtcggcga
     2461 tgctgcactc caccccgtcg cgcgccgcca gacccacggc aggcgcggcc gagagcacca
     2521 ccgggcgcgc catcgccatc gcttccagca ccttgttctg gatgccgcgc gcaatgcgca
     2581 ggggcgccac cacgaggtcg gcgtgggcga tgtaggggcg cacgtcgggc acggtaccgg
     2641 tgaccacgac atcctcgccg gcgagcgcct gcacggccgg cgccgggttc atgcccacga
     2701 tccagaagcg cacgccgggc atggcgcgac ggatcgccgg caggagctcg gtggcgaacc
     2761 agcacaccgc gtcgacgttg ggccagtagt ccatcgcacc gctgaaggcg atcaccggag
     2821 cgcccgccgg atagggcgaa gcccccgcgt tggcaggcgc gaagtactcg gcgtcgaccc
     2881 cgttctgcat gacgccgacg cggtcgccga cttcgggcgc cgcgcgccgg aagacctcgg
     2941 cctcggcctc ggtgacgaag aggctcgcct ccacgccggc tgcggcgcgg cgctcgaagt
     3001 cgagcaggcg ctcgccctcg cgccggtaca gccaggacat cggccagcgc cggtcggcgg
     3061 cgtactgcgt ccacttggcc gaatcgacgt cgcagaagtc gaccacgcgg cggctcagcc
     3121 cgggcacgtc gaggtactgc gccatcggcc ccgagaaggc caccgccgaa cggatgccgt
     3181 tctccgccac cacctgatcc acccagcgcg ccaggcgcgg gctgcggtaa tagggcaggc
     3241 tcagcgcctc gccgcgcagc aggccgcgca ggctcgcgaa gcggcggatg cccggctcga
     3301 tcgggatcac gcaggtctgc tcgcaccact ggcgcagcgc cgggatgtgg cggcggtcgt
     3361 ccgcatggtc gacgaagctg cccagccaca cccggtgcgt gcgggcgagc tggcgcagga
     3421 tgttgaacga ccgcagcttg tcgcccttgt tgggcgggta ggggatgcgg tgcgtgaggt
     3481 agaggagcgg cggccggctg tcctgcaccg ccctacccca ggttgcgcac gatgtgcggc
     3541 ccgagccggt tcgccacgcc gatcggcagg cgcttccaga gggcgatgaa ggcccggtac
     3601 ttggggttca tcgggttgtt ctgcgggatg ctgtcgcgct tgtacaggca gaactcgtac
     3661 gacagcggct gcggctcgaa gccccagttc ttcttgaacg cataggggcc ggtgccgacc
     3721 ttgctgcgac cgtagtcgaa gaccgtgcag ccgcgctcgc aggcgcgccg catcagctcc
     3781 cagtacttga agtcgttggc ggcgagctcg cgcgctgcaa ggtcgtcgcc cgcgtagtag
     3841 ggcagcacct cgtcgcggaa gtagaagctg agcacgctcg acagcggctt gccctcgggc
     3901 gaggtcaccg tgagcacttc gcaggcctca ccgaagcgct ccttgagctg ctcgaagaac
     3961 ttcctcggca gcgccggcgt gccgtggcga agcacgttgt cggcgtagag cgcgaagaag
     4021 cggtcgacac ccgcgtccac ctcgccgacc aggccgttct tgatgccctt gcgcaccatg
     4081 gcgcgctgct tgcgcgggat ggcgagcagg ttggcctcga cctcgggcag gatctccttg
     4141 cggaaggtga cgtagagttc ctggcgcggc cagtcctcgt ggcgcggctt caggttgcgg
     4201 aattcgaggt gctgcacgcc gagcgcctgc gccagcgtct cggccttgga ttcgagcgcg
     4261 tacagggtct ccgggcctgc atcctcggcc gccgcgatgc cgccgtagac gcagaagggt
     4321 agcgacacca gcgcatgacc gaacaggcgg ctgcgcacct cggcgagcgg cagcacgccg
     4381 acgatgtcgc cggcgcgctc ggcatacaga tagaaggtgc ggtgatcgaa cacctcttcc
     4441 atgagctcgc gccaggcgga caggtggaag aaggtggcct gcgggcagcg gcgcacgaat
     4501 tcgtcccagc gcagggcgtc ctcggccttg agcaggttca cgacgatcgg caatcggtcc
     4561 atggcgcgca tccggatcca gtcgggttca ggcggcgtcg cggaagactt cgtcggcgcg
     4621 cccccaggag aaatcggaga gaagccggtc gagccgggct tccgtgtgct gcaggttaat
     4681 gtagtgcctg aagcgggtct ttgccgtggc agcggtcaca cgcggctgct cgggatcgat
     4741 ttcccagggg tggaagtaga agatcgccgg gcggcggtcg gcggcattga cgcgcgcgat
     4801 ctgccagcgc gacagcgcat agggcaacag gcggaagaaa ccgccgccac ccgcgggcca
     4861 gttgcgtcca agcatgtgca cggtggtgat cggcacctcg accaggccgt tgggcaggcg
     4921 ccacgggaag cgcggcgcgt cggggatgcc gtagtggtcg tggcggaccg gatacacgct
     4981 cgagctgtag cggtagcccg cctcggcgat gcagtcgtgc gcccacaggt tggcctcgcc
     5041 gatcgagaag ctgggcgcgc ggtagccggt caccgcacta ccggtgatgt cctcgaggat
     5101 caccttggcc aggcggatgt cggccgtgaa ggcctcgggc gtcagatcgc tcgcgcgctg
     5161 gtgcgcgtag ccgtggctcg ccacctcgtg gcccgcggcg gcgatgcggc gcaccaggcc
     5221 gggatagcgc tcggcgagcc agcccagggt gaagaaggtg ccgcgcgcgc cgtggccgtc
     5281 gagcagcgcg aggatgcgat cgacgttgcg ctcgacgcgg cagggcacgc tgtcccactg
     5341 ctcgcgcgga aaatgcggcg cgagcgcgga gacctggaag tagtcctcga cgtcgcaggt
     5401 gaaggcgttg gtgatgcgtg cggccacctc aaccccccgg cgcggcgtgg accgtctcgt
     5461 gctcgacgag ccagtcgtgc agatgggcag cgatgcgctc ggcgcagcgt ccatcccagt
     5521 attcgggcac ccgcccggcc ttgccaccgg tggcgaggat ctcgcgcgcc gcggcgacga
     5581 tcgcctcggg gcggatgccg accagggtgt tggtgccctg ctccaccgtg atcgggcgct
     5641 cggtattctc gcggatggtc acgcagggca cgccgagcgc ggtggtctct tcctggatgc
     5701 cgccggagtc ggtcagcacc atgcgcgcac ccgacatcag gccgagcatc tcgaggtagc
     5761 cctgcggcgg caggatcagg aagccggagc cggcgaggcg atcgagcatg ccgaagcgct
     5821 cgaggttgtt gcgtgtgcgc ggatgcagcg cgacaaccag cggcaacgcc tcgctcaccg
     5881 tgcgcagggc gtcgatgatc gggccgaggg tgtcggcgct gtcgacgttg gacggacggt
     5941 gcagggtgac gacgccgtag ccgccggcga tccggctcgc gtccaggccg gccgcggcga
     6001 gcagcacgtc ggccggcacc gcgcgcggca ggctcgcgcg caggctgtcg atcatgacgt
     6061 tgccgacgaa aaccgcacgc tcggcgggga tgccttcgcg gacgaggttg tcgtgggcgg
     6121 atcgctcggt ggtgtagagc acgtcggaga tctggtcggt gaggatgcgg ttgatctctt
     6181 cgggcatgcg gcggtcgttg ctgcgcaggc cggactccac atggaccacc gggatgtggc
     6241 gcttgctcgc caccagcgcg caggcgagcg tcgagttgac gtcgcccacc accagcaccg
     6301 cgcgcgcgcc atgctcgtcg atgaccggct cgaagcgctt catcacctcg gcggtctgca
     6361 ccgcgtgcga gccggagccg accgcgaggt tgacgtcggg ctcgggcagg tcgagatcgg
     6421 cgaacaggcg gtccttcatc gccggatcgt aatgctgccc ggtgtgcacc agcagcgtgg
     6481 ggatcggcgg ctggtgggcg gcgaaggcgc ggatgatcgg cgccaccttc atgtagttgg
     6541 gacgtgcgcc gacgatgcag atcaccggcg cggccgagag gcgatgcgcg ctcatcgacc
     6601 ttcctcctcg gtcgcggcat cgcggcgcac cgcctgcagc aactggttga gcactgcgag
     6661 cgtggccgac accgactgct ccaggccggc cagacgtgcc tcgatgcgct gcaggtcgta
     6721 gctgccggcc atgccggcga cctggtcggc aagctcgggc gagacgcgca ggcgctcgac
     6781 tgccagcgcc tcgaccggcg gcatcgccgc agcgggctcg gcgtccctca gcaccgcgcg
     6841 tcccatgccg gccaccgggc gcgtacgcgg cggcgcgaac tcttccttga gctcggcgat
     6901 cacctcgcgc acctgcgcct cggtgaccac atggctctcg ccgagatagg cggcgaggag
     6961 cagacggtcg cacaaggtgt tgatggtgcg ggggatgccg ccggtgtggc cgtagatcgc
     7021 gttgaaggac ccgggctcga agcgcgggtc ctcgttccag ccgacgtggc gcaggcggtg
     7081 ctcgatgtag ccgcgcgtct cctgcaggtc gagcgggccg aggtgatagg aggcgatcac
     7141 gcgctggcgc agttgctgca tctcggcgcg ctgcatcatc tcgcggaact cgggctggcc
     7201 gacgaggaaa ctctgcagca gcgcatgatc ctcgagctgg aagttcgaca gcatgcgcag
     7261 ttcctcgacc gcggctgcgg agaggttctg cgcctcgtcg atgatcagca gcgcgcgctt
     7321 gtcggcggcg gcgagcgaca cgaggtaggt ctcgatcgcg agcagcaggc cggccttgtc
     7381 gaggttgcgg ctgggtacgc cgaaggcgcc ggcgaccatg cgcagcatgt cggaggcgtc
     7441 gatctgggtg ctgacgatgg tggcggcgac gaccttggcc ggatcgagct gctcgagcag
     7501 gctgcgcacg atggtggtct tgcccgcacc cacctcgccg gtgatgacga tgaagccctc
     7561 gctctggtgc aggccgtatt cgagataggc catcgcgcgc cggtgccccc gactgccgta
     7621 gaagaaggcc gggtcggggt tgagctggaa aggcttgccg ctgagtttga agaaggtttc
     7681 gtacatcggg tgtccgttca gaaccgcatg ccgaggatgg cggtgacgga attcgtcgtg
     7741 tagtcgctct cgccctcggt cttttcatat cggtagcgca gccccgcgcg cgtcctgggg
     7801 ccgagcgtac gatcgacgcc caccgtgccg gtcaggcgac gcagtgcgag gtcggcgtcg
     7861 gccgcactct cggaatccga gcgcgcgagc gtggcataca gcgaactcgt gccgctgagc
     7921 gtgtggctcc aggcgagcgt ggtggtggtg atgcgcaccc gctcggtgtc gcgcaggtcg
     7981 tcttccggaa gcaggccggt ggtcgaaccg atgcggctgc gatcgctgcg actggcggac
     8041 aagctcaagg tgttgcgcac cccgagcagc gagacgccgc cacggaacgc gcgctcgacg
     8101 taggcggcat tggagcgcag cacgccgaag gtgagacatt cgttgagggc ctgctcgcgc
     8161 tcgatcggat cggcgatgtc gcgtacgcgc atgaagcagt cccgatccgc gagcaccacc
     8221 gtgcccagcg tctgtgcgag cgaggagacg tcgcgcgtcg caccgaggaa gaagaccgcg
     8281 cggcgcatgc ggtggttgag ggcgagatcg tagccgcgcc cgaacacccg atcctcgacc
     8341 gtcgcggcga gcgaggtgcg gtcggtcggg taccagtcca ccccggcgcc aacgatcccg
     8401 ctgctgtcct tcctgccgtc cgcgtagtcg ttggactcgt gcccgacgat gccgcgcagg
     8461 cgcagctgcg gggagacgtt ggcgtacagc gtgccacgca ccgactcctc gccacgctgg
     8521 ccggtggcgg cggtgccgcc atcactttcg gtgcgggcat aggccaggcc ccagccgaaa
     8581 aaccgcaagc cgcccggatc ggcgaggctg gcctccacgg tgtccgcgct ctggtcgccg
     8641 agaccgctgc tgttggatcg cacgccgctg gacgaatagc tcaccgaacc gcgtgcagcc
     8701 tcgccgaagc gcaggtcgga ctcgagccgc ggggtgatcg accaatagcg gacttcgttg
     8761 tccttgtcga aaccgaacgg gtcgccgggc gcccgcccgc tgaaggcgga atagttgttg
     8821 cgggtgattc ccccgctgaa atcgaggaac agcagatcct ccagcgcctc gtaggcgccc
     8881 ctgccgtcga aggagaggta agtggagtcg tcctcgctcg cagaggaatg gacgaggttg
     8941 cgcaggcgcg cgctcaggct gccgttgaga cgccctgcgc tgcggctcac cgacacgccg
     9001 ggcgagacct cgagcagcca gtcccgaccg ccctcgcgat cgaggccggc gttgtcggtc
     9061 caggtgagtt cggtgtcgaa cgtgggcgtg acccgggtcg actgcgcgac cgctccggtg
     9121 gccgggcaca ggagcgcgag cacgcacgca ccggcgacga ggccggtacg ccggggctca
     9181 ggccgcctcg gaactggctt cgggcgcttt cccatatccg tatccgtatc catagccgta
     9241 gtagcccccc accgagcgat cacgcgactt gttgagcacc atcatgcgga tcgggcaggg
     9301 ctcgatcgct gcgagcgcct ggcgaacgtg ggcgtgggtg gtgcgctcgg cctccaccac
     9361 gaggaccacc tgtcccatgt gggtggcaag cacgcgcgat tcggtcgtga ccagcagcgg
     9421 cggcgagtcg aacacgatga tccggtcggc gtaacgatgc gccatctgct cgagcaactg
     9481 cgtcatgccg tccgaggcga gcagttcggt cgcgcgctgg tgcggcatgc ccgccggcag
     9541 gatggacagc ttgtcgatgt tggtcttgag caggacctcg gagaggtcct cgacctcgcc
     9601 ggcgagcacg tccatcagtc ccggctgcgg cggcagatcg aactcgcgca gcaccgaggg
     9661 acgcgacacg tcggcgtcca ccagcagcac cgtgtagtcg agctccatcg ccatgctgat
     9721 cgcgagattg atcgcgttga aggtcttgcc ctcgccagga agcgcgctgg aaatcatgat
     9781 caggttgccg ttatcgaccg ccgccggccc ctcgaggacg gcattggcca gcaagggacg
     9841 cttgatcagg cggaactcct cggcaatggc cgagcgcggc tggtcgggcg tgaccaagcc
     9901 gcgagcggcc aggcgcgcga gatcgatatg gacgctcttg ggctgggcgc gcaccggcgc
     9961 gggggtcgcg accaccggcg cattcgactc caccttgggc gcaagcggct tgtcctcggg
    10021 caggacgagc gcctccggca ccggagcgga ttcgacggcg ccctcgcccg ccgcactctt
    10081 gagttggtcg aggcgcttga ctgctttttc gataatgctc atccgggcac cccgttcaac
    10141 tctgcaggaa cacgatcgcg accgtaaccg cggcgaacac cccggcgtac gccaccagac
    10201 cgctgccgaa ggcgaacagc ccgttgcggc gacgacgacg acgctccgaa gtggtcagca
    10261 tggagacggt gccgagcacc ggcaggccgg tgatctcgcg cagctggcgc gcatcgctga
    10321 acgccggtcg cagctggctg atcaggaagg tgagcgcgaa ccccgccccc agacccacca
    10381 gcgcggcgag cggcagcagc agcagccggt tgggagccga gggtttggtg ggcagggtgg
    10441 gcggatcgat gatgcggaag tcggcgatgc ccgactgggt gctcatctcg gcggcgatgt
    10501 tcgccgactc ccgccgcgat accagctggt cgtagttgct cttgtggacg ctgtaatcgc
    10561 ggttgagctg cgccatctcg gtctcgagct cggggatccg cttggcgtcc tccgagaggc
    10621 tcgccaggcg gctctcgaac tcggccaccc gcgcacgcag cgacgcgacc cgggcctcgg
    10681 cctgcgccac cgcgagccgg gtctgctcga cccccgcact catggcgccg ggagcaccga
    10741 aggtactgcc cgccatgctc gcgctcagct cggccagctg cgccttcttc tgcttctcga
    10801 gatcctcgat gacgcggcgt gcaccgacca cgtccgggtg catctcggtg tagcgctgca
    10861 acatctcgtc gaggtttctc ttgagcaggt cgatgcgggc atcgatctcg ggcgtcgcca
    10921 ccatcggcgg cggcgcgatg cccgcatcgg acggcgctgc ggagagttgc tggcggagcg
    10981 cgtcgcgcga gttctcggcc tcgcgcagtt ccaggcgtgc ttcgcgcaat tgctcgttgg
    11041 tctcggcgat ctgggtgacg taatcgcgcg cgccggtacc cagcagcgcc atgttgcgca
    11101 ggcggaagtc cttgagccgg ttctcggcgt cggtcagctt ctgctcgtag ctgcggatct
    11161 ggtcctcgat gaagcgccgt gccgcgtcgg tatcctgacg cttgccgccg agaccggatt
    11221 cgacgaacag ggacagcagc gactgcacga cgcgctgcgc acgatgcggc tcgacgtctt
    11281 cgtacgagag cgtgaacagg ttgtcgccgc ggccggactg gatgcgcagc cggctggtca
    11341 ccgaggtgat cagggcctcg cgctgctgtg cgttctgcac cgtgaggtcg aggtcggcca
    11401 tggtgatcag cttctcgacg ttgggccggc tgatcagcgt gcggctgagc aagtcgatct
    11461 gctggctgat gttgggctgg acggcgagcc cggacatcag cgggcgcagc accgactggg
    11521 tatccacgtg gatgcgcgcg gacgactcgt agtggtccgg catgaagtag atcgccaggc
    11581 taccggcgat acctgcgacc cacgcgagcg caagccccca ccagcggaac cgccacatgc
    11641 cgcgcaggta gccgatgatc tgtgttacga gttcttccat gggtcacggt cgtgctgagc
    11701 gcccgccacc gaccttctgc gatcggcttg cgggcgtgta tggatggagg tcagaaccag
    11761 ctctgcggga tgatcaacac gtcgccaggg cgcatctcga cgttcgccga gacgtcgccg
    11821 cgcttgacga ggtcacggat gcgcacgctg tattgcttgt tgccctcggc ggtgcgcagg
    11881 atgctcgcgc cgttaccgtc ggcgaagtcc gtgagcccgc cgactgcgat catcacgtcg
    11941 agcagggtca tcttctgctt gtacgccagc gtctgcggat tggccgcctc gccgatcacg
    12001 cggatctgtt cgctgtaagg accgacgaag ccaccgacga tcaccgtcac gaccggatcg
    12061 cggatgaact tcgcaagctg cttctcgatg tcgcgggcca gcgtgctcga atccttgccc
    12121 atcgcgggca ggtcttcgac cagcggtgcc gtgatcttcc cgtccggccg gaccgggacg
    12181 gaggtggaca gctcggggtt gcgccagacc acgatattga cgctatcccc gggcccgatc
    12241 acgtagttgt agtccgcatc ggccgcggac gcgggggcag gcgggaagga agaggcacaa
    12301 ccaccgagca gtcccgccgc cacagcgaga acgaggaaat gcaacaccgc ggatgatgcg
    12361 cttttgacgc ttctgaacat gacaagcccc ttcgtaagtc ccggacggcg aatgatatac
    12421 gacgacgcga atgcgagggc catcgtacgg gcaccgcccg ccgcgacgcc aggcaggatt
    12481 gcacacaagc gcctacattt caggagacat gaagtcggct agattcacgg ctcgcccgtt
    12541 ccggattatc attgatccga tgggagtcgt ccgcgctcgc cgcgcgcgca ctccagacca
    12601 cccagaacgt aaacgaattg ggccactcca atgcgccaga tccactcttc gcggggcacg
    12661 aactgccccc cgcccgtacg cgacctcgct tacctagcgt tgtctggcgc cggattccaa
    12721 tcgccggaga cgtcgccgca tcagtggttc gacgagaaca ccgccgcact gcgcggcctt
    12781 tcgttcgaac gcagcggctt ccaggtctgc atcgccaacg gcagtgaagc gctcaagcgc
    12841 cagaccgcca agctgatcga gcgcatgtac tcggcgcgcg gcctttttcc ctacgggatg
    12901 cacgccaaca tcgacccgcg cgacatcacc gtggtcgctc tcctcgacgg ggaagcggtc
    12961 gcgacggcga cgatccgcgt cgatcacggt ggtggattgc tcgcggacac tttgtacagc
    13021 aaagagatcg accacgtgcg cgcgaccggg ggtcgagtgt gcgaaattac acagttggcg
    13081 atcgatcccg agcgcggatc gcacgaagcg ctggctggcg tgatgcaggc cgtgttcgtg
    13141 atcacccagg cgacgcggcg cgtcaccgac attttcgccg aggtcaatcc gcgccatgcg
    13201 ggattccatc agcgcgtatt cggctttcgt cgcgtgggcg tggagaagat ctgccccagg
    13261 gtcaacgctc cggccgtgct cctccatctg cccctgtccg agttcgagga gcgcgtcgcc
    13321 cgccacgaac gcctgatgca catgaacggc gatcgggttc atcgcctgct gccggacccc
    13381 gtccagtcca ggcagttgat ccaggcgatg accggtaagg cggcctgagc ccgcccgggg
    13441 cactcagaac atgtggcgca gttcgagata gacacgatcg ttccgatcgt aacggctgaa
    13501 aaaccccctt ccattcccgc cgaagacgtc gacgccaccg acgagcctcc atgccggggc
    13561 gaatttccag gtcagcttcg gtcgaatcag gtgatccgac cggttaatgc tggacagcgc
    13621 gaggacttcc gcctccaggt tcgtgcccgc gccatgaacg acctgcaagg tgacgccctg
    13681 ctcgtgccga tcggccatca aggccggaac atgctcctcc agccagcgcc cgtagtactg
    13741 cacgttgaag cgccagtcgc ccgcgggcac atcgaccccg agcgcatagt cgaccatgtc
    13801 ggtcggcagc agcccgatgg agaggcccgg accgctcgag aacgtattca ggctgcgacc
    13861 gtgcgtatgg accgcctcgc ccttcagcac gaagctgccg agatccttgc tgaaagtgcc
    13921 gccaagctgc ccgatccggt cgtgtcgcag gcgggccaca ccctggttca gggcgtatag
    13981 cgtcggcgag acatcgtagc tgcggtaata gaaggcgctg agatcccacc catcgacgag
    14041 tcgcgagacg cgcgcacccc agttcgagcc atccagatcc ttccccggac gatcctcgtc
    14101 cagcaccgta cctgcaggca accacgggaa gacgaagaaa tcgtcgccgg gctcgccgat
    14161 ctcgtcgaac gagggcttcg gcacccacag gaactctagg tgcgtttcgg caccgaaatg
    14221 ctcggcgcgc agcgcccact gcccgatccg catgccctcg agttccggct gcaggaaatc
    14281 ccgcatgtcg cgtgcggaca cgacgtcggc gaaaaagaag ccgaccatct cgccccatac
    14341 gatctgctgc cgcccggcac ggaattccca gtcgccatgg cccagatcga cataagcctc
    14401 gcgcaacacg aaatccgtgc ggcggtcgcg ccgcacggcg gccggataat gttcgtcctc
    14461 gaggtcgaaa gcaccgtcgg cctcggcgcg cgcgctcagc ttccatcgcg cccgcggcgc
    14521 cagctgcccg gcgacaccga gttccccacg cgcgcgcagg cgcgaccagc gctccggatc
    14581 ccggtatgtg tatgccgccc cgagttcggt gtaaccgttc cacttgaggc cgtccgcgcc
    14641 ctctgcaccg gacgccggcg ctccgacgtc gagcagcgcg tcgagttcgg cgtcggcctc
    14701 acccgcgtcc gcgtccgtca cgggttcttc ggcagcgggc tccccttctt ccacctccac
    14761 cgacaccggt tttggcgcga gctgcggccc cgccgcagtc gccggcacgg agagggcgcc
    14821 caggacaagg gcaagaacgg tcagacaagg cagcttcttc gacagtggca ttgcactcgt
    14881 tcctagattt cgcggatatc ctcgtcgcgc ggcgtccgcc catcgacgaa cacccagcgg
    14941 tcgtcggcct tcatgccgag cgcgatgatc tggccgtcgg cgatgcgcac cagccggtcg
    15001 gccatgttca tgaccttgcg gtcgtgggtg gaaaagacga aagtcgtccc cgactggcgg
    15061 ttgatgtcgc gcatcagctt gaggatgctg gtgccggtct tgctgtcgag gttggcggtg
    15121 ggctcgtcgg caaggacgat cttggagtgc gtggccagcg cgcgcgcgat cgccacgcgc
    15181 tggcgctggc cgccggagag ctggttcggc cgatgctcgg cgtatttctt caggccgact
    15241 accccgagat aatggcgcac ccgttccgcg cgctccttct tcgtgagctc gggcatctgc
    15301 agcaggggat actcgacgtt ctccgcggcc gacaggacag ggagcagatt aaaggtctgg
    15361 aagatgaagc cgatggtacg ggcacgcagg tcggccagtt cgtcgggcgt acggttcgac
    15421 acgtcgtgac cgtccacgac cacccgcccc gaactcggcg tgtcgatgca gccgatgatg
    15481 ttcagcaagg tcgacttgcc actgcccgaa ggccctgcga tcgccaggaa cacccctggc
    15541 tcgatctcga gcgagacgtc gtccagcgcc agcaccatct gctcgccgag acgaaaactc
    15601 ttgctcacgt gttcgatctg gacgatggcc atcaggacga tcgagaatga agataacttc
    15661 gaaaggatag cacccatgac gccaagctta cgatcccgcc gccgattcgt cgcctgcctc
    15721 gccggcagcc tgctgctgcc gacgctggcg catgcgaccg acgcccccgc gcctgccgag
    15781 cccgcacccc ccgagccggt cgacgcggtg gcgatcgatc tggtccagcg cgccgacgcc
    15841 atccgcttcc cgcgcgagag cttccagacc gaggtcgtcg tgcgcaacta taccgacggt
    15901 gcagaaggcg actcgcgcac ctaccgcatc ctctcgcgcg ggaacgaaaa caccatcgtg
    15961 ctgacgatgg agccggccac cgagcgtggc caggccttgc tgatgcgcgc ccgcgacctg
    16021 tggatcttca tgccgagcgt gtcgcagccg gtgcgcctgt cgctggccca gcgcctgacc
    16081 gggcaggtgg ccaacggcga cctcgcacgg gccaacctgg ctggcgacta cacgccgacc
    16141 atcgccggcc gcgaaacgat cgacggccag cccgtggtgg tgctcgacct caaggcggtc
    16201 gatcgcggcg tcacctatgc gcgcgtgcgc tactgggtgg gcgagcgcga ccagcgcccg
    16261 ctcaaggccg agttctacgc actctccggt cgtctgctca agaccgcacg ctacgaagaa
    16321 ttccgccaga tggccggcgc gatccgcccc acccgtctgg tcatggagga cgcgctcaag
    16381 aagggcgaga tctcggtgct gacctacgag aacatgaacg tgcgcgacct ccccgagcgc
    16441 atgttcaccc gcgagtacct gcgccgcctg cagcagtagg gtgtgcgccg agccggcagg
    16501 tcttcacacc accacgcgca cgtgcccgcc ggtcgcctcc tgcaggtggc gggcaaattc
    16561 cgcacgcacc ccatcttcgc catgcaccag gcgcacctcg cccgggagat ggcgcatgcg
    16621 ggtgacaaag cgggtcagat cgtcgcggtc ggcatgcgcc gagtagcccg acagcgtatg
    16681 gatgccggcg cggatgtcca cgcgttcgcc gtccagctcc acgtagccgc cgcgcggccc
    16741 gtaggcctgg atggcgtggc cgggcgtgcc gcgcgcctga tagccgacga aaagcacgtc
    16801 gtggcgcggg tcgccgagca tctcgcggag gtaattcacc acccggccgc ccgcgcacat
    16861 gccgctcgcg gcgatcacca ccgcaggctc gcggctgcgt gcgagcctgc gcacgttggc
    16921 gagatggtcc tcgtgactgt cgatcgccac cagctgatca taggacagcg gctggcgccc
    16981 ggcgcgcacg cgcgcgcgcg cctcggcgtc ccagaacggc tgcaactcgc ggtagagcgc
    17041 ggtgaagcgc tgcgccagcg gcgagtcgag gaagatccgc agcccgcccc aggcggcggc
    17101 atgcggcccg ggccgcatgc gctggcgatg caggatgtcc tcgaactcgt agagcagctc
    17161 ctgcgtgcgc ccgatgctga aggcgggcac gatcaccgtg ccgccgtcct cgagcgcatg
    17221 ctcgacgacc gcctgcagac gcgcacgccg gtcgcggcga ttctcgtgga cgcggtcgcc
    17281 gtaggtggtc tccaacacca gcacgtccgc ccgccagggc ggacgcggcg ccggcagcag
    17341 cggcgcgtgc ggcgccccga gatcgcccga gaacaccacc cggagcggct gcggccacgc
    17401 cgcgcccgag agctcgcatt cgacgtaggc cgagccgagg atgtggccgg cgcgctgcag
    17461 gcggatcctg cagctcgcgt ccgggctgcg atacaccagg tgccacttgc cgtagggcag
    17521 cgcgcggatg cgcgcctcca ccttgccgag gtagcgctcg atcagggcgc ggtcgcggct
    17581 cacgcccacc gcgaaggcgt cggcgagcac ggtgggcagc aggcgtgccg agggctcgct
    17641 gcacaggatc ggccccttga agccagcggc gagcagccac ggcaggcggc cgcaatggtc
    17701 gatgtggacg tgggtcagca ccagcgcgcc aacaccctcg agcgggaaat cgatctccag
    17761 ccggtcggcg gccgagcgcc cgtcgggcgc ggtctccgcg ccctggaaca ggccgcagtc
    17821 gaccagcagg ctgttgcgcc cctcgacgaa gagctggtga caggaaccgg tgacgccgtc
    17881 gcgcgcgccg tggtgcagga tggtcgggtt catgacgtca ttctagacgc ccatgcgccg
    17941 acgtccaccc gcggcggccc cgccgcacca gc
//
LOCUS       DAOQBS010000056        17792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2269_length_17792_cov_18.272763, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000056 DAOQBS010000000
VERSION     DAOQBS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 17792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17792
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2269_length_17792_cov_18.272763"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..2583)
                     /locus_tag="PKN25_08120"
     CDS             complement(16..2583)
                     /locus_tag="PKN25_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV90903.1"
                     /translation="MNRPESDRLLSEEAFSMPGNDETRVGEIIGRNLLECAPDEPLHE
                     AARRMSERRVSSILVVEDDRVVGIWTERDALRVDFDDPTTFALPVRAVMSSPVRVVST
                     ATPLHELTVRFREEHVRHYVVEDETGRRVGIVSQTDVVLNQGIEHYLKLRRVDALVKG
                     GLHALPAAAGLGEATRRMREGGVDAVVVDYGAADASAPAGRYGILTERDVTRLVAQRT
                     ADRALGELASRPLVTCRADDSLYRVRMLLAERHIRHIGVLDAEGELVDLVSFKDILSG
                     MELAYVHELHDALRARDLALSASQRDLYLAEKVIESSLEGVMVTDAHARILSVNPAFT
                     RMTGYAPEEVVGLNPSVLNSGRQSPAFYARMWDGLLGDGQWQGEVWNRRKTGEVYPQL
                     LHITAIRDREGVLTHYAALFTDISRLKETEARIRDLAYYDPLTGLPNRRLLDDRLQVE
                     LAHAARLRCRLAVMFVDLDRFKRINDSLGHAVGDKLLVEIAARLRASLRDDDTVARMG
                     GDEFLVVLNNLSGPDEAATMARRMVAELRRPVNVEGRELVVTTSVGVAVYPDDSRDAD
                     TLVKHADVAMYRAKDEGRNSFQLFEPAMNARSLERLALETALHRALPRDELQLYFQPV
                     MKAEGGELVAVEALLRWRHPDLGLVSPADFIPLAEDTGLIVPIGEWVLRSACEHHRRW
                     CEAGGAPLHMMVNISARQFREEGFAAMVGSVLAETGMTPGDLTLELTESMLMDDTDRA
                     LARLDHLRALGVCLAIDDFGTGYSSLAYLKRFPIDELKIDRLFVRGIDRNTRDAALVS
                     AIVSLGHSLGLRVVAEGVETAAHLDVLRRQGCDLAQGFHFSPPLPWEEFVKAYPPAG"
     gene            2746..3942
                     /locus_tag="PKN25_08125"
     CDS             2746..3942
                     /locus_tag="PKN25_08125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV90904.1"
                     /translation="MIRVLMVSDVFFPRVNGVSTSIATFRRALAAQGVEVRLIAPRYA
                     GEAEEPGIVRVAGRRLPLDPEDRLVDARAMHAAALREAAVCDLVHIQTPFLAHRAGLR
                     AARAQGRPVLLTYHTFFEEYLHHYLPFLPAGVLRGLARRVSRTQCNAVDAVVVPSTAM
                     HERLAGYGVRSPMEVLPTGIPLQDFAAGDGPHFRRLYDIPEDAPMALYIGRVAHEKNI
                     GFLLEVATRLKARRPDFVLVVAGEGPANARLQRQALAAGLGSTVRFVGYLERRRRLPD
                     CYAAADVFVFASRTETQGLVLLEAMAAGLPVVALSAMGTTDILRSERGARIAPDHPDG
                     FAAVVDVVLADPRLRRRLAAEARGYAAEWADGVMAGRLAALYRRLLAAPQSVPPVHAS
                     QVVSTR"
     gene            4020..4223
                     /locus_tag="PKN25_08130"
     CDS             4020..4223
                     /locus_tag="PKN25_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNV90905.1"
                     /translation="MNTTTLTITGMRDEECLRLVTNAIQDLPGIGWLEISLATGAATI
                     EHGAFVAEADIHQAIEDAGFGVR"
     gene            4296..>5750
                     /locus_tag="PKN25_08135"
     CDS             4296..>5750
                     /locus_tag="PKN25_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNV90906.1"
                     /translation="MTPTLRSFPGGLAFVDIETTGGPAQRESITEIGIVQVDEDGVRE
                     WSTLVRPASRIPETIQRLTGIDDDMVADAPRFEDIADEVFDRLDGRLFVAHNARFDHG
                     HLRAAFRRAGLDMRPQVLCTVKLSRRLFPDHRRHGLDHLIERHGLAVADRHRALGDAR
                     LLWQFWQKIHERFPPGHIDAAVRELIGHPSLPPHLDPEQIADLPDTPGVYLFYGERGG
                     ESSQPGGADEADAEAEADPLGPGRARTGARDRKRHAPLQDLPLYIGKSTRLRSRVLSH
                     FAADHSSDRELSLTQQVRRIEWIATAGEIGALLKEAELVKRLQPTHNRQLRRNRELCT
                     WRLATDIVGDWRLELVHAADLDFGRRDDLYGFFRTRREATNRLRALARDHALCPPLLG
                     LEKPPQGARCFDFQLKRCRGACHGGESPQAHALRLIEALHALKVEHWTWPGPVGLREG
                     EAIHVVDGWRWLGTATDEAMLADLLEAGRPAFDHD"
     assembly_gap    5751..5850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5851..6729
                     /locus_tag="PKN25_08140"
     CDS             <5851..6729
                     /locus_tag="PKN25_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV90907.1"
                     /translation="LAVVVFWSGFNLVSRFGATTSFSPFDIAAMRYGVSGMIALPFFL
                     RFVPMRDWPRHAVLACVGGLAYGLCVYWGFAFAPSAHAGVFVNGGIPFWTIVIMAVTS
                     GFRIARPTVVALLLSTAGLLLIGFESLLAPAQGSAWIGDLLFLAAALAWAIFGLLMRR
                     WQVKPQFGILGIASFSALVFMPIYLLWLPSGIGAASWGEIALQCVYQGVIASMLAAGF
                     YSYAVQKVGAGEASMMLALVPAFTAIGAYLILDEALGTTTIVGIVVVSAGALLGALPP
                     AALARVRGSGSAHSAR"
     gene            complement(6771..7214)
                     /locus_tag="PKN25_08145"
                     /pseudo
     CDS             complement(6771..7214)
                     /locus_tag="PKN25_08145"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
     assembly_gap    7445..7544
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7545..8098)
                     /locus_tag="PKN25_08150"
     CDS             complement(<7545..8098)
                     /locus_tag="PKN25_08150"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNV90908.1"
                     /translation="MGDNLALVEACASAGRLLPVYCHDPSADAPTRWGFVRRGPHRRA
                     FLEAALGDLDAALRARGSALLQLNGAPRAVLPALAEAVGAAVVVCEAIAAPEEEDELA
                     ALRAAGLKVKAIWQSTLLDPAALPFAAARLPKVFTAFRQAVESAGLQPPAPLPAPRTL
                     PPWPPLDRRPRRSDASRDLRAGRSP"
     gene            complement(8210..8356)
                     /locus_tag="PKN25_08155"
     CDS             complement(8210..8356)
                     /locus_tag="PKN25_08155"
                     /inference="COORDINATES: protein motif:HMM:NF021526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HNV90909.1"
                     /translation="MRRKGDLPTKPCAGCGRPFAWRKKWERVWDEVRFCSERCRREHG
                     KPGR"
     gene            complement(8375..9955)
                     /locus_tag="PKN25_08160"
     CDS             complement(8375..9955)
                     /locus_tag="PKN25_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HNV90910.1"
                     /translation="MSYGLVWFKRDLRLADHAALATAARRGPVLCVLIVEPSLWAQPD
                     AARQHYEFMLESARELHAGLARVGGRLHLLVGEAVAVLDRLHAAAPFDTLHSHEETGN
                     AASYARDRAVARWCRARGVRWHEPAQFGVVRRLDDRDRWQAAWEAQVAAPQVELPEPS
                     RLRFVALPAALQPGAGATWPDRGAIAAVRAPTAVALGLDAFEPPRRQRGGRHAALEVL
                     HDFLDARSGQYRGGISSPLKAPTACSRLSPYLAWGCLSLRELVQATRARVAALPEGDR
                     RRAGLAAFLSRLYWHCHFIQKLESEPTLEFRNLHRGYDGLREPEWNQAHFDALVGGRT
                     GWPLVDACVAMLRATGWLNFRMRAMLVSVAAYPLWLHWREVGLWLARAFLDYEPGIHW
                     SQLQMQSGTTGINTTRVYNPIKQARDHDPQGVFVRRWLPALRRVPDIWLFEPWRMPES
                     VQARCGVRVGEDIALPVVDLETATRAAKTRIHALRAQPEVRAAKAAIVERHGSRKPPQ
                     GRRKTVAGSASGQLDLGF"
     gene            complement(10043..11035)
                     /locus_tag="PKN25_08165"
     CDS             complement(10043..11035)
                     /locus_tag="PKN25_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004386048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV90911.1"
                     /translation="MNANPHLAIIGAGIAGLACARSLADSGVRVTVLDKSRGPGGRTS
                     TRRGEGWACDHGAQYFTARHPDFIEELAAWQAAGVAAPWPARLTVFDSDGRRGVHGEE
                     ARFVGTPRMTAPARHLARGLDLRVQLTVSALQRYPHGWEIATAERGMLPEAFDGVLLA
                     VPSPQAVPLLLPLSPALAATAQAARMQACWALMARFDAPLAVDFDAAFVNRGPLRWVA
                     RDRSKPGRDGLETWSLHASAEWSEAHIEDAPERVAVALVGAFEELGGRMPLGWAAHRW
                     RYAITESALDGGCAWDAAQALGLCGDWANGGRVEGAWLSGRALARRVLDGLAAG"
     gene            11284..11745
                     /locus_tag="PKN25_08170"
     CDS             11284..11745
                     /locus_tag="PKN25_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HNV90912.1"
                     /translation="MNVLLPDSVRRTANRLGYAGLIPFVVLAPASLLDAHHGITWSDA
                     LFAYGAVILSFVCALHWGFAMALPGLDERTRVRSLLWSVVPSLIAWPALLLVPSDAAV
                     LLVLGFVLHYVQDRRLARVAGLPEWYLPLRLRLTTVACIALISGAFVQWPY"
     gene            11799..13163
                     /locus_tag="PKN25_08175"
     CDS             11799..13163
                     /locus_tag="PKN25_08175"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNV90913.1"
                     /translation="MNTAASAIDPSLPVADAARSAVVPGGRLSRLARLGSLATGVAGG
                     MLAEGARQLAAGKRPKVSELVLTPANARRVAEQLAQLRGAAMKVGQLMSMDAGSLLPP
                     ELADILARLREDARTMPMSQVVEVLETHWGKGWEQGFERFSFTPCAAASIGQVHRART
                     RDGQELAIKLQYPGVRRSIDSDVDNVATLLRVSGLLPKALDLAPLLAEAKRQLHEEAD
                     YRREAESLHRFGGLLGDAEHFVLPRAVDALTRSDILAMSWVEGVAVETLADPQAADQA
                     LRDRVASLLIGLLFRELFEFRLIQTDPNFANYRFDAASGRVVLLDFGATRPYAEPVVE
                     AYRRLMAGSVRGDRVTMGEAAQAIGYFQDNIHAHQRDAVIDLFEIACEPVRHPGAYDF
                     GTSDLPLRLRDAGLKLSMERDFWHTPPADALFLHRKLGGLYLLAARLRARVDVAALAA
                     PWLG"
     gene            13261..14400
                     /locus_tag="PKN25_08180"
     CDS             13261..14400
                     /locus_tag="PKN25_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNV90914.1"
                     /translation="MRDILIVGAGKIGTVIADLLAGSGDYAVTVADRDSAAVERVGAE
                     LARVQACALDVADADALAAELEGRWAVIDAGPFDIGMRIAAAAVAQRVHYLNLTEDVA
                     STRRVRELARGAHSALIPQCGLAPGFISIVAHDLAARFEELRDVRMRVGALPKYPSNG
                     LKYNLTWSTDGLINEYLNPCEAIVDGVRREMPALEELEHFSLDGDDYEAFNTSGGLGT
                     LCDTLEGRVRNLNYRTVRYRGHRDVMKLLLHDLRLGERRALLKDILESAIPVTMQDVV
                     LVFVTVSGRREGLLMQETFARKLYAAEVNGRLRSAIQLTTASALCAVLDLLAAGRLPQ
                     AGFVRQEDVDFCDFVTNRFGRHFLTDSEEVRCAASGVLAGPASGM"
     gene            14420..15925
                     /locus_tag="PKN25_08185"
     CDS             14420..15925
                     /locus_tag="PKN25_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNV90915.1"
                     /translation="MLSNARFLPLGLDAPRLREGALAVHSPIDGSLLARLAPQDGATT
                     DAAIARSVAAFEAWRRVPAPRRGELVRRFAQALREHKALLAELVTLECGKIRSEGEGE
                     VQEMIDICDFAVGLSRQLHGLTIASERPGHALRESWHPLGPVAIVTAFNFPVAVWAWN
                     AAIALVCGDSLLWKPSERTPLCALACQRLLEQAAAGMEEVPRGLSAVIVGGAERAVQL
                     ADDRRVALLSATGSCAMGRALAPRVAQRLGRSLLELGGNNAVIVAPSADLELALRAIV
                     FGAVGTAGQRCTSTRRLFVHAAVREQLLERLRAVFAGLVVGDPRAADTLVGPLIGGEA
                     FTRMQAALAAARAAGARIDGGERVLAERFPAAWYVRPALVEHPPSVTNSMEEVFAPLL
                     NCFEYAELEDAIARQNAVPQGLSSAIFTTDLREAERFLSTTGSDCGIANVNAGTSGAE
                     IGGAFGGEKDSGGGREAGADAWRAYMRRMTATINYSDALPLAQGVRFEPAG"
     assembly_gap    16006..16105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16135..16737)
                     /locus_tag="PKN25_08190"
     CDS             complement(16135..16737)
                     /locus_tag="PKN25_08190"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNV90916.1"
                     /translation="MSTASIPLRQLSGLPATPARLSESALVIVDAQNAYRGGVMQLEG
                     VEPALDECARLLARARALKIPVVHIQHDSGPGSPFDPSAECGAIADKVAPVAGEAVVV
                     KHYPNSFVGTELDAHLKAAGVKNLVVVGFMTHMCINSTARGAFNLGYAVAVPAAATAT
                     RALPAPDGSVLAAAALQVAALTALADLFAVVVADGAAIPD"
     gene            16852..17742
                     /locus_tag="PKN25_08195"
     CDS             16852..17742
                     /locus_tag="PKN25_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV90917.1"
                     /translation="MNWDDLRFLVTLGREGTLAATARRLAVDQTTVARRLRALEEGLG
                     TALFERDEGLWRPTAVGARVLERAARIEEDVAGITRLAEAGAGAVGGVVRITAVSALI
                     GEWLVPRLPGLYARHPELRVDLVASNDNLNVARREADIAIRLARPASGDFLIRKLAGV
                     GFALYGAARGDLAVSAGDWVAYNEDLDHTPEMRRLHAMLGEGRIRLRSNSLRGLVRAV
                     AGGIGHGLLPCFVADADPGLVRLSGAEPILYRELWMLIHPDARPLARVAATADWLVER
                     FDEAAAGFAGRVPSPRSAPP"
BASE COUNT         2328 a         6506 c         6200 g         2458 t
ORIGIN      
        1 cgaggaccgc ggcgctcagc ccgccggcgg gtaggctttg acgaactctt cccagggcaa
       61 gggcgggctg aagtggaagc cttgcgccag gtcgcagccc tggcggcgca gcacgtcgag
      121 gtgggcggcg gtctcgacgc cctcggcgac cacgcgcagg cccaggctgt ggccgagcga
      181 gacgatcgcc gaaaccagcg ccgcgtcgcg cgtgttgcgg tcgatgccgc gcacgaacag
      241 gcggtcgatc ttgagttcgt cgatcggaaa gcgcttgaga taggcgagcg acgaataccc
      301 cgtgccgaaa tcgtcgatcg ccaggcacac gccgagcgcg cgcagatggt cgaggcgcgc
      361 gagcgcgcgg tcggtgtcgt ccatcagcat gctttcggtg agctccagcg tcaggtcgcc
      421 cggcgtcatg ccggtctcgg cgagcacgct gccgaccatc gcggcgaagc cttcctcgcg
      481 gaactggcgc gccgagatgt tcaccatcat gtgcagcggt gcgccgcccg cctcgcacca
      541 gcgccggtga tgctcgcagg cgctgcgcag cacccattcg ccgatcggca cgatcaggcc
      601 ggtgtcttcg gcgagcggga tgaagtcggc cggcgacacc aggccgaggt cggggtggcg
      661 ccagcgcagc aaggcctcga ccgcgaccag ttcgcctccc tcggccttca tcaccggctg
      721 gaagtagagc tgcagctcgt cgcgcggcag ggctcggtgc agcgcggtct cgagcgcgag
      781 gcgctcgagc gagcgcgcgt tcatcgccgg ctcgaagagc tggaagctgt tgcgcccctc
      841 gtccttggcg cggtacatcg ccacgtcggc gtgcttgacc agggtgtcgg cgtcgcgcga
      901 gtcgtccggg tacacggcga cgcccacgct ggtcgtcacc accagctcgc ggccctcgac
      961 attcaccggg cggcgcagct ccgcgaccat cctgcgcgcc atcgtcgccg cctcgtcggg
     1021 gccggacagg ttgttgagca cgacgaggaa ctcgtcgcca cccatgcggg cgacggtgtc
     1081 gtcgtcgcgc aggctggcgc gcaggcgggc ggcgatctcg accagcagct tgtcgcccac
     1141 cgcgtggccg aggctgtcgt tgatgcgctt gaagcggtcg aggtcgacga acatcaccgc
     1201 cagccggcag cgcaggcgtg cggcgtgggc gagctcgacc tgcaggcggt cgtcgagcag
     1261 gcggcggttg ggcaggccgg tgagcgggtc gtaataggcg aggtcgcgga tgcgcgcctc
     1321 ggtctccttg agccggctga tgtcggtgaa cagcgccgcg taatgggtga gcacgccctc
     1381 gcggtcgcgg atcgcggtga tgtgcagcag ctgcggatac acctcgccgg tcttgcgccg
     1441 gttccacacc tcgccttgcc actggccgtc gcccagcagg ccgtcccaca tgcgggcgta
     1501 gaaggcgggg ctctggcggc cggagttgag taccgaggga ttgagcccga cgacctcttc
     1561 cggcgcatat ccggtcatgc gcgtgaaggc ggggttcacc gagaggatgc gcgcgtgcgc
     1621 gtcggtgacc atcacccctt cgagcgagct ctcgatcacc ttctcggcca ggtagaggtc
     1681 gcgctgcgag gcgctcagcg cgaggtcgcg cgcgcgcagg gcgtcgtgca gctcatgcac
     1741 gtacgccagc tccatgccgc tgaggatgtc cttgaagctg accaggtcga ccagttcgcc
     1801 ttcggcgtcg agcacgccga tgtggcggat gtggcgctcg gcgagcagca tgcgcacgcg
     1861 gtacaggctg tcgtcggcgc ggcaggtgac gagcggccgg ctggcgagct cgccgagcgc
     1921 gcggtcggcc gtgcgctgcg ccaccagccg ggtgacgtcg cgctcggtga ggatgccgta
     1981 gcgccccgcg ggcgcggagg cgtccgccgc gccgtaatcc accacgaccg cgtccacccc
     2041 gccctcgcgc atgcgccgcg tcgcctcgcc cagccccgcc gccgcgggca gcgcatgcag
     2101 gccgcccttg accagcgcat ccacccgacg cagcttgagg tagtgctcga tgccctggtt
     2161 cagcaccacg tcggtctgcg acacgatgcc cacgcgccgg ccggtctcgt cctcgaccac
     2221 gtagtggcgc acgtgctcct cgcggaagcg cacggtcagc tcgtgcagcg gcgtcgccgt
     2281 cgacaccacg cgcacaggcg agctcatcac ggcgcgtacc ggcagggcga aggtcgtggg
     2341 gtcgtcgaaa tcgacccgca acgcgtcgcg ctcggtccag atgcccacca cacggtcgtc
     2401 ctcgaccacc aggatcgagc tcacccggcg ctcgctcatg cgccgcgcgg cctcgtgcag
     2461 gggctcgtcc ggggcgcact cgagcaggtt gcggccgatg atctcgccga cgcgggtctc
     2521 gtcgttgccg ggcatggaga aggcctcctc gctcaggagg cggtcggatt caggacggtt
     2581 catgggggac tcggcacagg ctttagattc tgtataacgg ctccagccga ctgaaacctg
     2641 aggcggctgc gcacattgtc gcacccgtgg ccctgcaggc ggaccccggc taacccgcag
     2701 ggaggccttc accctgcggt aacacgcccg gcgcatgctg gccgaatgat ccgcgtgctg
     2761 atggtctccg acgtcttctt tccgcgtgtg aacggcgtgt ccacgtcgat cgcgaccttt
     2821 cgccgcgcgc ttgccgcgca gggcgtcgag gtgcgcctga tcgcgccgcg ctacgccggc
     2881 gaggccgagg agcccggcat cgtgcgcgtc gccgggcgcc gcctgccgct cgaccccgag
     2941 gaccgcctgg tcgatgcgcg cgcgatgcat gccgccgccc tgcgcgaggc cgccgtctgc
     3001 gacctggtgc acatccagac ccccttcctc gcccatcgtg ccggactgcg cgccgcgcgc
     3061 gcccagggca ggccggtact gctgacctat cacaccttct tcgaggaata cctccaccac
     3121 tacctgccct tcctgcccgc gggcgtgctg cgcgggctgg cgcggcgggt gtcgcgcacg
     3181 cagtgcaatg cggtggatgc ggtggtggtg ccctcgaccg cgatgcacga gcgcctcgcc
     3241 ggctacggcg tgcgcagccc gatggaggtc ctgcccaccg gcatcccgct gcaggacttc
     3301 gccgccggcg acggtccgca tttccgccgc ctctacgaca tcccggaaga tgcgccgatg
     3361 gcgctctaca tcggccgcgt cgcgcacgag aagaacatcg gcttcctgct cgaggtcgcc
     3421 acccggctga aggcgcgccg ccccgacttc gtcctcgtgg tcgccggcga ggggccggcg
     3481 aacgcccgcc tgcagcgcca ggcgctcgcc gccgggctgg gcagcaccgt gcgcttcgtc
     3541 ggttatctcg agcgccgtcg ccgcctgccc gactgctacg ccgccgccga cgtgttcgtg
     3601 ttcgcctcgc gcaccgagac gcagggcctg gtgctgctcg aggcgatggc cgccgggctg
     3661 ccggtggtcg cgctgtccgc gatgggcacc accgacatcc tgcgctccga gcgcggcgca
     3721 cgcatcgcgc ccgaccatcc cgacggcttc gccgccgtgg tcgacgtcgt gctcgccgac
     3781 ccccgcctgc gtcggcgcct cgccgccgag gcgcgcggct acgccgcgga gtgggccgac
     3841 ggcgtgatgg ccgggcggct cgccgcgctc taccgcaggc tgctcgccgc gccgcagtct
     3901 gtgccgccgg tgcacgcgtc gcaggtcgtc tccacccgct gagacgctcg gcaggcggcc
     3961 cgggcttgcg cgcgtccgtg cctgcgcaca agatgcgccg atcggacacg gagctgccca
     4021 tgaacaccac cacgctcacg atcaccggca tgcgcgacga ggagtgcctg cgcctggtga
     4081 ccaatgccat ccaggacctg cccggcatcg gctggctgga gatctcgctc gcgaccggcg
     4141 cggccaccat cgagcacggc gccttcgtcg ccgaggccga catccaccag gccatcgagg
     4201 acgccggctt cggcgtgcgc tgaacacggc gggcacggga acccgcgcgc ccgcgcacgc
     4261 tcgaagagcg accccatccg ccggttcgcc ccctcttgac gcccaccctc cgcagctttc
     4321 ccggcggcct cgcctttgtc gacatcgaga ccaccggcgg ccccgcccaa cgcgaatcca
     4381 tcaccgagat cggcatcgtc caggtggacg aggacggcgt gcgcgagtgg tcgacgctgg
     4441 tgcgtcccgc gtcgcgcatc cccgagacca tccagcgcct caccggcatc gacgacgaca
     4501 tggtcgccga cgcgccgcgc ttcgaggaca tcgccgacga ggtcttcgac cgtctcgacg
     4561 gccgcctctt cgtcgcccac aacgcacgct tcgaccacgg ccacctgcgc gccgccttcc
     4621 gccgcgccgg gctcgacatg cggccgcagg tgctgtgcac cgtcaagctg tcgcgccggc
     4681 tgttccccga ccaccgccgc cacggtctcg accacctcat cgagcgccac ggcctggcgg
     4741 tggccgaccg ccaccgtgcg ctcggcgacg cccggctgct gtggcagttc tggcagaaga
     4801 tccacgaacg ctttccgccc ggtcacatcg atgccgcggt gcgcgaactc atcggccacc
     4861 ccagcctgcc cccccacctc gaccccgagc aaatcgccga cctgcccgac acgccggggg
     4921 tgtatctgtt ctacggcgag cgcgggggcg agagcagcca gcctgggggc gccgatgaag
     4981 ccgacgccga agccgaggcc gatccgctcg gacccggcag ggcgcgcacc ggcgcgcgcg
     5041 accgcaagcg ccacgcgccg ctgcaggact tgccgctcta catcggcaag agcacgcggc
     5101 tgcgcagccg cgtgctgtcg cacttcgccg ccgaccacag cagcgaccgc gagctcagcc
     5161 tcacccagca ggtgcgccgc atcgaatgga tcgcgaccgc cggcgagatc ggcgcgctgc
     5221 tgaaggaagc cgaactggtc aagcgcctgc agcccaccca caaccgccag ctgcgccgca
     5281 accgcgagct gtgcacctgg cggctcgcca ccgacatcgt cggcgactgg cggctggagc
     5341 tggtgcatgc ggccgacctc gacttcggcc gccgcgacga cctctacggt ttcttccgca
     5401 cccgccgcga ggccaccaac cggttgcgcg cgctcgcccg cgaccacgcc ctgtgcccgc
     5461 cgctgctcgg cctggagaaa cccccgcaag gtgcgcgctg cttcgacttc cagttgaagc
     5521 gctgccgtgg cgcctgccac ggcggcgaat ccccccaggc ccacgccctg cgcctgatcg
     5581 aggccctgca cgcgctgaag gtcgagcact ggacctggcc cggcccggtc ggcctgcgcg
     5641 agggcgaggc catccacgtc gtcgacggct ggcgctggct cggcaccgcc accgacgaag
     5701 ccatgctcgc cgacctgctg gaggccggcc gcccggcctt cgaccacgac nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctcgccgtgg tggtgttctg gtccggcttc
     5881 aacctcgtgt cgcgcttcgg cgccaccacc agcttcagcc ccttcgacat cgccgcgatg
     5941 cgctacggcg tctccggcat gatcgcgctg cccttcttcc tgcgcttcgt gccgatgcgc
     6001 gactggccgc gccatgccgt gctcgcctgc gtcggcgggc tggcctacgg gctgtgcgtg
     6061 tattggggct tcgccttcgc gccgagcgcg cacgcggggg ttttcgtgaa cggcggcatc
     6121 ccgttctgga ccatcgtgat catggcggtg acgagcggct tccgcatcgc ccgcccgacc
     6181 gtggtcgccc tgctgctctc gaccgccggc ctgctgctga tcggcttcga gagcctgctc
     6241 gccccggcgc aggggagcgc ctggatcggc gacctgctct tcctcgccgc cgcactggcg
     6301 tgggcgatct tcggcctgct gatgcggcgc tggcaggtca agccgcagtt cggcatcctc
     6361 ggcatcgcga gcttctcggc gctggtcttc atgccgatct acctgctctg gctgccctcc
     6421 ggcatcggcg ccgcgagctg gggcgagatc gcgctgcagt gcgtgtacca gggcgtgatc
     6481 gcctccatgc tcgccgccgg cttctacagc tacgcggtgc agaaggtcgg cgccggcgaa
     6541 gcctcgatga tgctggcgct ggtgcccgcg tttaccgcga tcggcgccta tctgatcctg
     6601 gacgaggccc tcggcacgac caccatcgtc ggcatcgtgg tggtgtcggc cggggccctg
     6661 ctcggcgcgc tgccgccagc ggcgctggcc cgcgtgcgcg gctcgggctc ggcccacagc
     6721 gcccgatagg cgccgtccgc gtcgtacatc gccgcctgcc ggtccggatc gaagcgccgc
     6781 ccgccacgcg gatcggtgcc gcggccggcg atgtagagcc agttgccctg gttgctatac
     6841 acgtcgtaat cgaccagctg cgcctcgaac cacgccgcgc cggcacgcca gtcacagccg
     6901 aggtcgtgga tcaggtagct ggcgacgatc tggcgcatgc ggttcgacag ccagccggtg
     6961 gccgcgagct cgcgcatgcc ggcgtcgatg aagggatggc cggtgccgcc cacgcgccag
     7021 gccgcgaacg caacggcgtc gtgcgcgggc tgcgctgcgc cttcgttcag cccgcgcgcc
     7081 cggtacagac ggcggccgtg ctgcagatgc aggaggcgga agtagtcgcg ccacagcagc
     7141 tcgaaccaca gccagtagct tccgtcgctc ggcccctgct cgatctcgtg gcggcgcagc
     7201 gcggccaagg ccacccgcgc cgacaaggcg ccctgcgcca gccagggcga gaacttgctg
     7261 gagaagtcga cgccgctcag cccgttgcgc gtcgccttgt agtgccgcgg ccgatcgccg
     7321 gcgaaatagc gtgccagatg cgcgagcgcc gcacgctcgc ccccggcgaa tgccggctgc
     7381 caccacggaa acgaggcctg ttcgccgatt tccacggtga gcgcatcggc ggccccgcag
     7441 gagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcgag cgcccggcgc
     7561 ggagatcgcg acttgcgtcg ctcctgcggg gccgtcgatc gagcggtggc cacggcggca
     7621 gggtccgcgg cgccggcagg ggcgcgggcg gctgcaggcc ggcggactcc actgcctggc
     7681 gaaaggcggt gaacaccttg ggcaggcgcg ccgccgcgaa aggcagcgcc gcggggtcga
     7741 gcagggtgga ctgccatatc gccttcacct tcagcccggc ggcgcgtagc gcggcgagtt
     7801 cgtcctcctc ctcgggtgcc gcgatcgcct cgcacaccac cacggccgcc cccacggctt
     7861 ccgcgagcgc cggcagcacc gcgcgcggcg cgccgttcag ctgcagcagc gcgctgccgc
     7921 gcgcccgcag ggccgcatcc aggtcgccca gcgccgcctc caggaaggcg cgccgatgcg
     7981 gcccgcggcg cacgaagccc cagcgcgtcg gtgcatcggc ggaggggtcg tggcagtaaa
     8041 ccggaagcag gcgcccggcg gacgcacagg cctccaccag cgcgaggttg tcacccacgc
     8101 gcagatcgtt gcgaaaccag aagatcacca cgtccgaaag catcgacacc cacccgcaaa
     8161 gccctcgagg cccgcctgtt ctcagcggcc tgccgcaaaa agtcgtcgtt cagcgccccg
     8221 gcttgccatg ctcccgccgg cagcgctccg agcaaaagcg cacctcgtcc cacacgcgct
     8281 cccacttctt ccgccacgcg aacggccgcc cacaccccgc gcatggcttc gtcggcagat
     8341 cccccttgcg ccgcatccgc atcgccttgc cccctcaaaa acccaggtcc agctgtcccg
     8401 acgccgaccc cgccaccgtc ttgcgccgcc cctgcggcgg cttgcgcgag ccatggcgct
     8461 cgacgatggc cgccttcgcc gcgcgcacct cgggctgggc gcgcagtgcg tggatgcgcg
     8521 tcttggcggc gcgcgtggcg gtctccagat cgaccaccgg caacgcgatg tcctcgccga
     8581 cgcgcacgcc gcagcgcgcc tgtaccgact ccggcatgcg ccagggctcg aacagccaga
     8641 tgtccggcac ccgccgcagt gccggcagcc agcgccgcac gaacacgccc tgcgggtcgt
     8701 ggtcgcgcgc ctgcttgatc gggttgtaca cccgggtggt gttgatgccg gtggtgccgg
     8761 actgcatctg cagctggctc cagtggatgc cgggctcgta gtcgaggaag gcgcgcgcca
     8821 gccacaggcc gacctcgcgc cagtgcagcc agagcgggta ggccgccacc gacaccagca
     8881 tcgcgcgcat gcggaagttg agccagccgg tcgcgcgcag catcgccacg caggcgtcga
     8941 ccagtggcca gccggtgcgc ccgcccacca gcgcgtcgaa atgcgcctgg ttccattccg
     9001 gctcgcgcag gccgtcgtag ccgcggtgca ggttgcggaa ctccagcgtc ggctcgctct
     9061 ccagcttctg gatgaagtgg cagtgccagt acaggcggct gaggaaggcc gccaggccgg
     9121 cgcggcggcg gtcgccctcg ggcagcgcgg cgacgcgcgc gcgggtggcc tgcaccagtt
     9181 cgcgcaggct caggcagccc caggccaggt agggcgacag ccgcgagcac gcggtgggtg
     9241 ccttcagcgg cgaggagatg ccgccgcggt attgcccgct gcgcgcgtcg aggaagtcgt
     9301 gcagcacctc cagcgccgcg tgccgcccgc cacgctggcg ccggggcggc tcgaaggcgt
     9361 cgagcccgag ggccacggcc gtcggcgcac gcacggcggc gatcgcgccg cgatccggcc
     9421 aggtcgcgcc ggcgcccggc tgcagggcag cgggcaacgc aacgaagcgc agccgcgagg
     9481 gctcgggcag ctcgacctgc ggcgcggcga cctgcgcctc ccacgctgcc tgccagcggt
     9541 cgcggtcgtc gagccggcgc accacgccga actgcgccgg ctcgtgccag cgcacgccgc
     9601 gcgcccggca ccagcgcgcc actgcgcggt cgcgcgcgta gctcgcggcg ttgccggtct
     9661 cctcgtgcga atgcagggtg tcgaagggcg ctgcggcgtg caggcgatcg agcaccgcga
     9721 cggcctcgcc caccagcagg tgcaggcgac cgccgacgcg ggccagcccg gcgtgcagct
     9781 cgcgtgcgct ttcgagcatg aactcgtagt gctggcgcgc ggcgtcgggc tgcgcccaca
     9841 gcgacggctc gacgatcagc acgcacagca ccggcccgcg ccgcgcggcc gtggccaagg
     9901 cggcgtgatc ggcgaggcgc aggtcgcgct tgaaccagac caggccgtag ctcacggcgt
     9961 gctcctgcgc gtgcgtgcgc gagcgggacg cgtcgcgggc gcggtcatgc tcgaccccgg
    10021 cagcgcattc atgcgcgcac gctcagcccg ccgcgaggcc gtcgagcacc cggcgcgcga
    10081 gcgcgcgccc gctcagccag gcgccctcca cgcggccgcc gttggcccag tcgccgcaca
    10141 ggcccaaggc ctgcgcggca tcccaggcgc agccgccgtc gagcgcggat tcggtgatcg
    10201 cgtagcgcca gcggtgcgcc gcccatccca gcggcatgcg cccgccgagt tcctcgaagg
    10261 cgccgaccag cgccacggcg acgcgctcgg gcgcatcctc gatgtgcgcc tcgctccact
    10321 ccgcgctcgc gtgcagcgac caggtctcca gcccgtcccg ccccggcttg gagcggtcgc
    10381 gcgcgaccca gcgcagcggc ccgcgattga cgaaggcggc gtcgaaatcc accgccaggg
    10441 gggcatcgaa gcgcgccatc agcgcccagc aggcctgcat ccgcgccgcc tgcgccgtcg
    10501 cggcgagcgc cggcgacaag ggcagcagca gcggcaccgc ctgcggcgag ggcaccgcga
    10561 gcagcacgcc gtcgaaggcc tcgggcagca tcccgcgctc ggcggtggcg atctcccagc
    10621 cgtgcgggta gcgctgcagc gcactcaccg tcagctgcac gcgcaggtcc aggcctcgcg
    10681 ccaggtggcg ggcgggcgcg gtcatgcgcg gcgtgccgac gaagcgcgcc tcctcgccgt
    10741 gcacgccgcg gcggccgtcg ctgtcgaaca cggtgaggcg cgccggccac ggcgccgcca
    10801 cccccgccgc ctgccaggcc gcgagttcct cgatgaagtc cgggtggcgc gcggtgaagt
    10861 attgcgcgcc gtggtcgcag gcccagccct cgccgcggcg ggtgctcgtg cgcccgcccg
    10921 ggccgcgact cttgtcgagg acagtgacgc ggactccgga gtcggcgagg ctgcgcgcgc
    10981 aggccaggcc ggcaatcccg gcgccgatga ttgcaaggtg tggattggcg ttcatgggcg
    11041 atccgggagg aaggggggag ttggacacga gcttgaatat actctttttg tcctggacaa
    11101 aataggattg gcttcataag atgaactcaa gcgcggggcg tgcatggcca agtcgcgcaa
    11161 gccgactccg agcctccgtt ggcggcgcag ggccggcggg gccggccacg acgcccaccc
    11221 cggactccgc tctgtccctc cgcccccaag cctgcccttc ctcctccccc gcgagccgcg
    11281 atcatgaacg tcctgcttcc cgactccgtc cgccgcaccg ccaaccggct cggctacgcc
    11341 ggcctgatcc ccttcgtggt gctcgccccc gccagcctgc tcgacgccca ccatggcatc
    11401 acctggagcg acgcgctgtt cgcctacggc gccgtgatcc tcagtttcgt gtgcgcgctg
    11461 cactgggggt tcgcgatggc gctgcccggg ttggacgaac gtactcgcgt gcgcagcctg
    11521 ctgtggagcg tggtgccctc gctgattgcc tggccggcgc tgctgctggt gccgtcggac
    11581 gccgcggtgc tgctggtgct cggcttcgtg ttgcactacg tgcaggaccg ccgtctggcg
    11641 cgcgtggccg gtctgcccga gtggtatctg ccgctgcgcc tgcgcctcac caccgtcgcc
    11701 tgcatcgcgc tgatcagcgg cgccttcgtg caatggccgt actgaagtca gcacttgccc
    11761 acccgagccc ggcccgaatc tccaccgagg agcacgccat gaacactgcc gccagcgcca
    11821 tcgacccgag ccttcccgtc gcggatgccg cgcgcagcgc cgtggtaccg ggcggccgcc
    11881 tgtcccgtct cgcccgcctg ggcagtctcg ccaccggggt ggcgggcggc atgctcgccg
    11941 agggtgcgcg ccagctcgcc gccggcaaac gacccaaggt gagcgagctg gtgctcaccc
    12001 cggccaacgc ccgccgcgtc gccgaacagc tcgcgcagct gcgcggtgcg gcgatgaagg
    12061 tcggccagct gatgtcgatg gacgccggca gcctgctgcc gcccgaactc gccgacatcc
    12121 tcgcccgcct gcgcgaggac gcgcgcacga tgccgatgag ccaggtggtc gaggtgctgg
    12181 agacgcactg gggcaagggc tgggagcagg gcttcgagcg cttttccttc accccctgcg
    12241 cggcggcctc gatcggccag gtgcatcgcg cgcgcactcg cgacggccag gagctggcga
    12301 tcaagctgca gtatcccggc gtgcggcgca gcatcgacag cgacgtggac aacgtcgcca
    12361 ccctgctgcg cgtctccggg ctgctgccca aggcgctcga tctcgcgccg ctgctcgccg
    12421 aggccaagcg ccagcttcac gaggaggccg actaccgtcg cgaggccgag agcctgcacc
    12481 gcttcggcgg cctgctcggc gatgcggaac acttcgtgct gccgcgcgcg gtcgatgcgc
    12541 tcacccgcag cgacatcctg gcgatgagct gggtggaggg cgtggcggtg gagacgctcg
    12601 ccgacccgca ggcggccgac caggcgctgc gcgaccgcgt ggcgagcctc ttgatcggcc
    12661 tgctgttccg cgagctgttc gagttccgcc tcatccagac cgaccccaac ttcgccaact
    12721 accgcttcga cgccgccagc ggccgtgtgg tcctgctcga cttcggcgcc acccggccct
    12781 acgccgagcc ggtggtcgag gcctaccgcc gcctgatggc gggctcggtg cgtggcgacc
    12841 gcgtcacgat gggcgaggcg gcgcaggcga tcggctactt ccaggacaat atccatgccc
    12901 atcagcgcga tgcggtgatc gatctcttcg agatcgcttg cgagccggtg cgccaccccg
    12961 gcgcctacga tttcggtacc agcgacctgc cgctgcgcct gcgcgatgcc gggctgaagc
    13021 tgagcatgga gcgcgacttc tggcacaccc cgcccgccga tgcgctcttc ctgcaccgca
    13081 agctcggcgg cctgtacctg ctcgcggcgc ggctgcgcgc gcgcgtggac gtggcggcgc
    13141 tggcggcgcc ttggctgggc tgacgaggcg ccggcgcccc gcggcccgcg ccgcgcaacc
    13201 atttccccgc acgcagggtc tcaaggcctg aaaccagcca atccgcgcgg ggagggcgtc
    13261 atgagggaca tcctgatcgt cggcgcgggc aagatcggca cggtgatcgc cgatctgctc
    13321 gccgggagcg gagactatgc ggtgacggtc gcggatcgcg actccgccgc ggtcgagcgc
    13381 gtgggggcag agctcgcgcg cgtgcaggcc tgcgcgctgg acgtcgccga tgccgatgcg
    13441 ctcgccgcgg agctggaagg gcgctgggcg gtgatcgacg ccgggccctt cgacatcggc
    13501 atgcgcatcg ccgcggccgc ggtggcgcag cgggtgcatt acctcaacct caccgaggac
    13561 gtcgccagca cgcgccgggt acgcgagctc gcccgcggcg cgcacagcgc gctgatcccg
    13621 caatgcgggc tggcgcccgg cttcatctcc atcgtcgccc acgacctcgc cgcgcgcttc
    13681 gaagaactgc gcgacgtgcg catgcgcgtc ggcgcactgc ccaagtaccc ctccaacggc
    13741 ctcaagtaca acctgacctg gagcaccgac ggcctcatca acgagtacct caacccctgc
    13801 gaggcgatcg tcgacggggt gcgccgcgag atgccggcgc tcgaggagct cgagcacttc
    13861 tcgctcgacg gcgacgacta cgaggccttc aacacctcgg gcgggctggg tacgctgtgc
    13921 gacacgctcg agggccgggt gcgcaacctc aactaccgca ccgtgcgcta ccgcggccac
    13981 cgcgacgtga tgaagctgct gctgcacgat ctgcgcctgg gcgagcggcg cgcgctgctc
    14041 aaggacatcc tggagtcggc gatcccggtg accatgcagg acgtggtgct ggtcttcgtc
    14101 accgtcagcg gccggcgcga gggcctgctg atgcaggaga ccttcgcgcg caagctctat
    14161 gcggccgagg tcaacggccg cctgcgcagc gcgatccagc tcaccaccgc gagcgcgctg
    14221 tgcgcggtgc tcgacctgct cgccgccggc cgcctgccgc aggcgggctt cgtgcgccag
    14281 gaggacgtgg atttctgcga cttcgtcacc aaccgcttcg gccgccactt cctgaccgac
    14341 agcgaggagg tgcgctgcgc cgccagcggc gtcctcgccg ggccggcctc cggcatgtga
    14401 ctccgaggag gaccccagca tgttgagcaa cgcccgcttc cttcccctcg gcctcgacgc
    14461 cccccgcctg cgcgagggcg cgctggcggt gcatagcccg atcgacggca gcctgctcgc
    14521 gcgcctcgcg ccgcaggatg gcgccaccac cgatgccgcg atcgcgcgca gcgtggcggc
    14581 cttcgaggcc tggcgccgcg tgccggcgcc gcgccgtggc gagctggtgc gccgcttcgc
    14641 gcaggcgctg cgcgagcaca aggccctcct cgccgaactg gtcaccctcg agtgcggcaa
    14701 gatccgcagc gagggcgaag gcgaggtgca ggagatgatc gacatctgcg acttcgcggt
    14761 cggcctgtcg cgccagctgc acggcctgac catcgcctcc gagcgccccg gccatgcctt
    14821 gcgcgagagc tggcacccgc tcggcccggt ggcgatcgtc accgccttca acttcccggt
    14881 cgcggtgtgg gcgtggaacg cggcgatcgc gctggtgtgc ggcgacagcc tgctgtggaa
    14941 gccctccgag cgcaccccgc tgtgcgcgct cgcctgccag cgcctgctcg aacaggcggc
    15001 ggcgggcatg gaggaggtgc cgcgcgggct gtcggcggtg atcgtgggcg gcgccgagcg
    15061 cgccgtgcag ctcgccgacg accgccgcgt ggcgctgctc tcggccaccg gcagctgcgc
    15121 gatggggcgg gcgctggcgc cgcgggtggc gcagcggctc gggcgcagcc tgctcgagct
    15181 cggcggcaac aacgcggtga tcgtggcgcc gagcgccgac ctcgagctcg cgctgcgcgc
    15241 catcgtgttc ggcgcggtcg gcaccgccgg ccagcgctgc accagcaccc gccggctgtt
    15301 cgtgcacgcg gccgtgcgcg agcaactgct cgaacgcctg cgcgcggtgt ttgccggctt
    15361 ggtggtgggc gatccgcgtg cggccgacac cctggtcggg ccgctgatcg ggggcgaggc
    15421 cttcacccgc atgcaggcag cgctcgccgc ggcgcgcgcg gcgggggcgc gcatcgatgg
    15481 tggcgagcgc gtgctcgccg agcgtttccc cgcggcctgg tacgtgcgtc cggccctggt
    15541 cgagcatccg ccctccgtga cgaacagcat ggaggaggtc ttcgcgccgc tgctgaactg
    15601 cttcgagtac gcggagctgg aggatgcgat cgcgcgccag aacgcggtgc cgcagggcct
    15661 gtcgtcggcg atcttcacca ccgacctgcg cgaggccgag cgcttcctct ccaccaccgg
    15721 cagcgactgc ggcatcgcca acgtcaatgc gggcaccagt ggcgccgaga tcggcggcgc
    15781 cttcggcggc gagaaggaca gcggcggcgg gcgcgaggcg ggtgccgatg cctggcgcgc
    15841 ctacatgcgc aggatgaccg cgacgatcaa ctactccgac gcgctgccgc tggcgcaggg
    15901 ggtgcgtttc gagccggccg gctgacgtcc ggccgcgcgt ggaacaagcg gtgatcgcga
    15961 cttgcgtcgc tcctacagga gggcggcacg gtttcccgcc ggggcnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnggtct ctcgggtgtt ccccgcgtcg gcgctcagtc
    16141 cgggatggcc gcgccgtcgg ccaccacgac cgcgaacagg tccgcgagcg cggtgagcgc
    16201 ggcgacctgc agggcggcgg ccgcgagcac gctgccgtcg ggcgcgggca gggcgcgggt
    16261 ggccgtggcc gcggcgggca cggcgaccgc gtagccgagg ttgaaggcgc cgcgcgcggt
    16321 ggagttgatg cacatgtggg tcatgaagcc gaccacgacc aggttcttga cccccgcggc
    16381 cttcaggtgc gcgtcgagct cggtgccgac gaaggagttc gggtagtgct tgaccaccac
    16441 cgcctcgccg gccaccggcg ccaccttgtc ggcgatcgcg ccgcattcgg cgctcgggtc
    16501 gaagggcgag ccggggcccg agtcgtgctg gatgtgcacc accgggatct tcagcgcgcg
    16561 cgcgcgcgcc agcaggcggg cgcattcgtc cagggccggc tccacgcctt cgagctgcat
    16621 cacgccgccg cggtaggcgt tctgggcgtc gacgatgacg agcgccgact cggacaggcg
    16681 ggccggggta gcgggcagac cggaaagctg acgaaggggg atggatgcgg tgctcatggg
    16741 gggctccttg tggggtcgag aaacgatggt ggcagcgtac aggcggccga cggcctgcgt
    16801 aaatgcacat tcctgctcta cactgctgca gatttgcatg gattccggca catgaactgg
    16861 gacgatctgc gtttcctcgt caccctcggc cgcgagggca cgctcgccgc cacggcgcgc
    16921 aggctggcgg tcgatcagac cacggtggcg cgccgcctgc gcgcgctgga ggagggcctg
    16981 ggcacggcgc tgttcgagcg cgacgagggt ttgtggcggc cgaccgcggt gggcgcccgc
    17041 gtgctggagc gggctgcgcg catcgaagag gacgtggccg gcatcacgcg cctggccgag
    17101 gcgggtgccg gcgcggtcgg cggcgtggtg cggatcaccg cggtgagcgc cctgatcggc
    17161 gagtggctgg tgccgcggct gcccggcctg tacgcccgcc atccggagct gcgcgtcgac
    17221 ctcgtcgcca gcaacgacaa cctcaacgtc gcgcgccgcg aggccgacat tgccatccgc
    17281 ctcgcgcgtc cggcgagcgg ggacttcctc atccgcaagc tcgccggggt cggcttcgcg
    17341 ctctacggcg cggcgcgcgg cgacctggcg gtgagcgccg gcgactgggt ggcctacaac
    17401 gaggacctcg accacacccc ggagatgcgc cgcctgcacg ccatgttggg cgagggccgc
    17461 atccgcctgc gctcgaacag cctgcgcggc ctcgtgcgag cggtggccgg cggcatcggc
    17521 cacggcctgc taccctgctt cgtcgccgat gcggaccccg gcctggtccg cctgtccggc
    17581 gcggagccga tcctgtaccg cgagctgtgg atgctgatcc accccgacgc ccgtccgctc
    17641 gctcgcgtcg ccgcgaccgc ggactggctg gtggagcgct tcgacgaggc ggcggcgggt
    17701 ttcgccggca gggtgccgtc tccccggagc gcgccgccct agcaccggcg cggcggtctt
    17761 gccgcggagg cttcagccgc cgagcagggc ga
//
LOCUS       DAOQBS010000057        16946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2476_length_16946_cov_16.468652, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000057 DAOQBS010000000
VERSION     DAOQBS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 16946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16946
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2476_length_16946_cov_16.468652"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1190)
                     /locus_tag="PKN25_08200"
     CDS             complement(147..1190)
                     /locus_tag="PKN25_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNV90918.1"
                     /translation="MQRLSAPALVAAALALPSLALAQSNVTLYGLIDLNLGYEKSGSQ
                     RYEGIGHGELNGSRLGFRGTEDLGNGNKALFVLESGFDPSTGVAEQGGRLFGRQSFVG
                     LENGLGRVTLGRQYSPAFDALDPFDATGNADRSAGLLIRKAGAVKPAYETRFDNMIKV
                     RSAKLAGFELEGGYWFGKESGTDSTARQEGDGHGIAMLYANGALAGSLVTQTVQRDAS
                     GGKVRTDGFALSYDFGIVKPYFAWSQDKERGSVGNGKARSWDLSAEIRLDPANTVAIS
                     YADRDESDGAASEDASGWSAYYLHALSKRTTLYAGYSQLDNEVGANYAIGNLTPAAGD
                     KHQVMMAGMRHRF"
     gene            complement(1313..2803)
                     /locus_tag="PKN25_08205"
     CDS             complement(1313..2803)
                     /locus_tag="PKN25_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HNV90919.1"
                     /translation="MDGTSGSRPANPGTDDREHGAAARAVAAPGHGGSLRQRVLVWLL
                     PPLAVLLLINAFFTHRSALDAVNQAYDRTLTASVRAIGERTHSLGGQIQVDIPYAAFE
                     GFEADAAERIFHAVFDPAGGLVTGYADLRPPELPADDGSVRIFDLTHAGETVRLAAMR
                     KRLYDPALRGGDAVLIVFAETTGARRALAHDLFVASLQRQSLLVAVGAILVLAALGTA
                     FRPLLRVRDHMRARDEEDLTPVRMERVPSEIAPLIDAINHHMERLSAMLAARRRFLAD
                     AAHQIRTPLAVLTTQAEYGLRQTDPEEMLATFASLLRSTRAARRLANQMLTLSRAEAV
                     NGLIGERAALDLAGLVKEVAMELAPLALKRDIDLAYEGIGHALTMQGNAPMLREMVSN
                     LIDNAIRYSPPGSAVTVAVAAFGEDAVVSVCDQGPGIPTAERERVFQRFYRILGQGDS
                     EGSGLGLAIVREIALAHGGRVKLRDGAGGRGLCVEVELPLGSTPSA"
     gene            complement(2805..3494)
                     /locus_tag="PKN25_08210"
     CDS             complement(2805..3494)
                     /locus_tag="PKN25_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV90920.1"
                     /translation="MRLLLVEDHAELAEWVARSLRHAGFVLDVLNRGDHADQSLRTER
                     YDLVILDLSLPGLDGLEVLRRLRNRESGPDKPAPTPVLVLTARGSTEERVHGLNLGAD
                     DYLPKPFELAELEARIKALLRRAGNQVPLVRLGRLEFDTTTRMAAVDGQALALTPREL
                     AVLEALLARVGRPVAREALFEKVFEFDADARVEAIEIYVSRLRKKLEGAGVAINTLRG
                     LGYLLAEKPAA"
     gene            3638..4744
                     /locus_tag="PKN25_08215"
     CDS             3638..4744
                     /locus_tag="PKN25_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV90921.1"
                     /translation="MRIRGGRAALKTVLAVVAAVAIAPVRAEPIADRFDQARLEGVVV
                     VYAATDLEVVKPVIDDFEALHPGVRVQYHDMHSAELHARVVDEARRGLAGADVVWSSA
                     MDLQVKLVNDGHAQPHRSAETAALPRWAVWKDEAFGTTYEPAVIVYNKHLLGTTEVPD
                     SHAELIRLLDRDPAPLRGRIATYDPERSGLGLLLHTQDAQANPIVFWQLARGMGRQGL
                     EQHAASSEMLDRVAAGKLVLAYNVLGSYAHRRARSDPALGVALPRDYTLVLSRVAFIV
                     RGARHPAAARLWLDHLLSTRGQALLAANLGLLPVRTDAGTAGADSAAALLHHNLQHAF
                     RPIRIGSGLLAYQDQAKKQAFLRQWDAATRPASE"
     gene            4870..5928
                     /locus_tag="PKN25_08220"
     CDS             4870..5928
                     /locus_tag="PKN25_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HNV90922.1"
                     /translation="MSALSTLRLAAWRQILPTLALATLAGWLAERLHLPLPWMIGPLF
                     AVAGARMADRDLRPLPGGRQTGQWAIGAALGLYFSPEVVAQLSLHAPLVIAAALGSLV
                     LGMAYAFILLRLGGVDAPTAFFAALPGGASEMANLADRHGGAVDRIAAAHAIRVMMVV
                     SVLPFALTFAGVQGSDVYVPLARDVDAQHFPLLVGASLLGVGAFKLLRIANAWVLGPL
                     LGVALATLAGVPLSALPPWVVNGGQLLIGCALGCRFSRAFFRAAPRFMSVAALTGAMS
                     IVLAFALATLLGLVAEVPLSTLALATAPGGVSEMCITAKVLQLGVPLVTVCHVLRVVV
                     LTVGAQWSFGIFRRLVAV"
     gene            complement(5968..7044)
                     /locus_tag="PKN25_08225"
     CDS             complement(5968..7044)
                     /locus_tag="PKN25_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HNV90923.1"
                     /translation="MTDLFTPLQAGDLALPNRVLMAPLTRCRAEDGHVPGALMATHYA
                     QRASAGLLIAEATMAMEGNSAFWHEPGIHSEAQVAGWKKVTDAVHAAGGRIVLQIWHG
                     GRACHPLLNDGRQPVAPSALAITGDEVHTPQGKQPYVVPRELRDDELPDIVAGFARAA
                     ENARAAGFDGVEVHGANGYLLDEFLRDGSNQRGGPYGGPRENRARLLFEVLDAVCKVW
                     GGGRVGVRLSPLNSYNDMIDSDPIGLASWLAGCLNDWRLAYLHLMRGDFFGQQHGDVV
                     TPVRAAYRGVLIGNMGYSADEANAAIAAGKLDAVAFGTAFLANPDLPERIRRKAALNA
                     PDPATFYTPGPKGYTDYPTLADGA"
     gene            complement(7041..8384)
                     /locus_tag="PKN25_08230"
     CDS             complement(7041..8384)
                     /locus_tag="PKN25_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNV90924.1"
                     /translation="MNGPGARTATSLRPLSPAQAAAGATPRVGQPSPLILFGAFDRHN
                     LGDLLLGRIAAELAARLLPGRELRFAGLAVRDLRADGGARVEALAELAAEWKPPRRDT
                     PPPGLLQVGGEILDCSAWEAAVMLLDNAGAARAIALYDHDPAARAAWARHALGVPRGL
                     PYLAAHAALPPGTRSVHTGIGGVGFAALPAALRAEALAELRAAAGVQVRDRCTRDALA
                     AAGLDAALVPDPAVLVERLLGAEVAHHAATGEVAALRARFARGYVAVQLAAEFGDDAT
                     LATLAGELAAIQRERDLGIVLFCAGRAPWHDDPDVLQRLRRRLPDPEAAVIAASPHVM
                     DLCALIANAALCCASSLHARIVADAFARPAIGLLPGGAPQAAKLRAWLDTWHPPRRHH
                     LAASGNLRALARSVLDEAPAERKAQAVRLADLAETAARAAFARLFDPIDPEDKTP"
     gene            complement(8398..9198)
                     /gene="fetB"
                     /locus_tag="PKN25_08235"
     CDS             complement(8398..9198)
                     /gene="fetB"
                     /locus_tag="PKN25_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HNV90925.1"
                     /translation="MVMELSVLDLALAALLILALAGLSLRLGLGIEQRLVVAAARTTI
                     QLLLIGLVLKLLFDNAHFLWVAAMAAVMITAAGREVMARQKRRFVGWWGFGLGTLSMF
                     VSSFTVSVLALLVVIGNTPWYAPQYAIPLLGMVLGNTMNGIALGLDRLTQEASARRTV
                     LETRLALGQNWNETIADTRREAIRVGMIPIINAMAAAGIVSLPGMMTGQILAGTAPVE
                     AVKYQILVMFLIAGGTGFGTVVAVSLGARRLFDERQRLRLDRLRTGAD"
     gene            complement(9192..9869)
                     /locus_tag="PKN25_08240"
     CDS             complement(9192..9869)
                     /locus_tag="PKN25_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV90926.1"
                     /translation="MPALALRQLRTRHIGPVDLDVAAGECVCIRGASGSGKSLLLRAI
                     ADLDPHAGEALLDGRACSSMPGPAWRRRVALVMAEGQWWADTVGEHFPAGCTPAWLDR
                     VDLPAGALDWQVARCSTGERQRLALLRTLMQGPAVLLLDEPTGNLDEDSTARVEAVLD
                     DYRREHAAALLWVSHDPRQAARVARRRFLLEDGKLVEEEEGGKAAADKGKAAGADRPP
                     DAEGAAW"
     gene            10063..10449
                     /locus_tag="PKN25_08245"
     CDS             10063..10449
                     /locus_tag="PKN25_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90927.1"
                     /translation="MYRDPRFSGIFQTLGGPAAKPPTLLQKVVGAVVMAGVFMLALTF
                     SVAFFAVLLTAGVAIWGWVWWKTRALRKAMRAQMDAQAAAGGARAGGYGRAAGDRGLI
                     IEGEVIREVEIEEDPRRDGPGGRTPS"
     gene            complement(10488..13820)
                     /gene="mscK"
                     /locus_tag="PKN25_08250"
     CDS             complement(10488..13820)
                     /gene="mscK"
                     /locus_tag="PKN25_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HNV90928.1"
                     /translation="MRPLIRVFLLLLFTLLAPWAAAEAPPRPQPDELRARAQALADRK
                     LPEAEQRAAQQALEQAQASLASADDHRRRLDSLKQTLADAPAQIAEARRQLAALPAAA
                     PVAAPAADTPLARLEERLAEQEAALADWRRRLDEADSLLVAARTGPERAQTEISTGQV
                     RMAAIEAMLASGREAGRDSRALSPERRDALAAEWHALDAQLALRRQELAGNSGLIDLG
                     LARQELARLQLARLEEAARALQDAISRQRASQAEQTVQALADVSADASHDDLLAREAA
                     INQQLSEYLLGATEKLDALTRRNQAVSQQLDVLNRSTTLIDEQIGVLQGSVLLSRILL
                     EQRRALPAPTTDAGLADQIADIRLYQFEINKQREQLSIPAAHAERLLADAGEADPAVR
                     EPLLALLATRSELLERLNRELNAVLAQSITLQLAERQLLVSADRLRATLDKQMFWIPS
                     NRPLDLAWLQAVPAALAKQFAAIPWGSAVGELWAGLQARPFLFLPVLLLSVGLFARRR
                     WLRAQLDSLHADIGYVRRDSQLHTPRAILLNMLLTLPVSLLLALGGIALQMDARGQNA
                     ALGAALMDMAQAWLVFRTAHRLLTPGGVAERHFQWPVERVRALHRNLNKLGLVVVAVV
                     AVVAIAERQPESLGIDVLGILILLVGYATMAWLLVRGLLHTRLEDRPSPLRVFVGLAL
                     GLMPLALIGALVMGYYYTALKLTGRLVDTLYVLILWRLAEGLLVRALGVAAHRIDHQR
                     AVARREAAEREGRAGADSEPAAVAEAPMLDIARINQQSLRLLHLALLSGLAVALYWVW
                     ADLLTVVSYLDNITLYEYASGSGETAGVVPISLADLLGALVIAAIAFMLATNLPGLLE
                     VLVLSRLQLAQGSAYAITTLLSYVIVGVGIASTLGTLGVSWDKLQWLVAALSVGLGFG
                     LQEIFANFVSGLIILFERPLRIGDVVTIGNVSGTVSRIQIRATTITDFDRKEMIVPNK
                     AFITGQLTNWSLTDTVTRVTIKLGVAYGADLETTRRLLLAIAAENARVLKDPEPLVFF
                     LNFGPSTLDHELRVHVRELGDRNAAIDEINREIDRRFREAGIEIAFNQMDVHIRSIAD
                     GREALLQRTAVPPPAGSPGQQG"
     gene            14037..15230
                     /locus_tag="PKN25_08255"
     CDS             14037..15230
                     /locus_tag="PKN25_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HNV90929.1"
                     /translation="MDYQIQRLKVLGAGIFSLMLALGVARFAYTPLLPLMQAQAGLGL
                     AEGGWLAAINYTGYLSGALLAASISDLVLKDRLYRIGMVVAVLTTLMMGLTTDFTVWA
                     VSRYLAGLSSAAAMLLGTGLILNWLIRHNHRHELGIHFAGIGLGIAGCSVAVALMSLW
                     LDWRAQWFVFTAIACVLLVPALRWLPAPDTSGLTRSGAPMHDDPPSPLFLRIFMASYF
                     CAGVGFVVSATFIVAIVNRLPGLEGQGSWSFLAIGLAAMPACIVWDFIARRTGALNAL
                     ILAAVLQIVGILLPVVVGGSLGAIAGALLFGGTFVGMVSLVLTMAGRYYPTRPAKMMG
                     KMTISYGVAQILGPAVTGWLGETFGSYAGGLWFAAAMMGVGTVLLVLLKIVDRRDAQA
                     AAGVA"
     gene            complement(15206..16057)
                     /gene="nudC"
                     /locus_tag="PKN25_08260"
     CDS             complement(15206..16057)
                     /gene="nudC"
                     /locus_tag="PKN25_08260"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HNV90930.1"
                     /translation="MSLPSSLPPSGDPRPARTLVFRGHQLLVDLAATPTAGPDSWRAG
                     LVALRTLDFPVTTGEAWTVVEVERTIEAPDGHAFQHLKSLLDTLDGETLALAARALEL
                     LEFDRAHRFCGACGAPTTLRTDPPARRCSAPDCGREHFPRVSPVVIVAVERGAEILLG
                     RSPHFPPGLYSTLAGFVDAGESAEQAVHREIFEETGLRIRAPRYFSSQAWPFPHALML
                     GYQAEYAGGDIVCAPGEIEDARFFHVDALPPLFPIRYAMANQLLRDFCLRHGRPWPPA
                     QATPAAA"
     gene            16359..>16946
                     /locus_tag="PKN25_08265"
     CDS             16359..>16946
                     /locus_tag="PKN25_08265"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNV90931.1"
                     /translation="MSAGKSKIIYTLTDEAPLLATCAFLPIIRTFTGPAGVEVEKADI
                     SVSARVLAEFPEYLSEDQRVPDTLAELGRLTLEPDTNIIKLPNISASVAQLKACVKEL
                     QEKGYKIPDYPEDPKTDEEKALRVRFGKCLGSAVNPVLREGNSDRRAPAAVKNYAKKH
                     PHSMGEWKQWSQTHVSHMHHGDFYHGEKSMTLDRAR"
BASE COUNT         2319 a         5780 c         6341 g         2506 t
ORIGIN      
        1 cggagacagc cgcagcgcaa ggcggctgcg gtcgaggtcg ccgtgcaccg tagcgcagaa
       61 ggccgcgcgt cgacgcaaaa ggcaggtgca gcggaagatt tccgcgtgca cctgccctgc
      121 gtgaaagcgt cgccctggcg gatggatcag aagcggtggc gcatgcccgc catcatcacc
      181 tggtgcttgt cgccggcggc cggcgtcagg ttgccgatcg cgtagttggc gccgacctcg
      241 ttgtcgagct gcgagtagcc ggcgtacagc gtggtgcgct tggacagcgc gtgcaggtag
      301 taggccgacc agccgctggc gtcctcgctc gcggcgccgt cggattcgtc gcggtcggcg
      361 tagctgatcg ccaccgtgtt cgccggatcg aggcggatct ccgcgctgag gtcccagctg
      421 cgcgccttgc cgttgcccac gctgccgcgc tccttgtcct gcgaccaggc gaaatagggc
      481 ttcacgatgc cgaagtcgta ggacagggcg aagccgtcgg tgcgcacctt gccgccgctg
      541 gcgtcgcgct gcacggtctg cgtgaccagg gagccggcga gcgcgccgtt ggcgtagagc
      601 atcgcgatgc catggccgtc gccctcctgg cgcgcggtcg agtcggttcc gctctccttg
      661 ccgaaccagt agccgccctc gagctcgaag ccggcgagtt tcgccgagcg gaccttgatc
      721 atgttgtcga agcgcgtctc gtaggcgggc ttgaccgcgc ccgccttgcg gatcagcagg
      781 ccggcgctgc ggtcggcgtt gcccgtggcg tcgaagggat cgagcgcgtc gaaggccggg
      841 gaatactggc ggcccagggt cacgcggccg aggccgtttt ccaggccgac gaaggattgg
      901 cggccgaaca gccgcccgcc ttgctcggcc acgccggtcg aggggtcgaa gccgctctcg
      961 agcacgaaca gggccttgtt gccgttgccg agatcctccg tgccgcggaa gccgagccgc
     1021 gagccgttga gctcgccgtg gccgatgccc tcgtagcgct gcgagccgga tttctcgtag
     1081 ccgaggttga ggtcgatcag gccgtagagg gtgacgttcg attgggccag cgcgagcgat
     1141 ggcagggcga gggcggcggc caccagggcc ggcgcggaaa ggcgttgcat gggagtctcc
     1201 tcgatcgttc ggatgtgcgc gccggatgga tcgggcgcgt ggcgcgcatt gcagcgccga
     1261 cgcctttcga tcggctttca gcgcgatccg gagacgagga gaagagcgac gttcaggcgc
     1321 ttggcgtgct gccgagcgga agttcgacct ccacgcacag cccgcggccg ccggcaccgt
     1381 cgcgcagctt cacccgtccg ccgtgggcga gcgcgatctc gcgcacgatc gccagcccca
     1441 gcccgctgcc ttcgctgtcg ccctggccga ggatgcggta gaagcgctgg aacacgcgct
     1501 cgcgctcggc ggtggggatg ccgggtccct ggtcgcagac gctcaccacc gcgtcctcgc
     1561 cgaaggcggc gacggcgacg gtcaccgccg agccgggcgg gctgtagcgg atggcattgt
     1621 cgatcaggtt ggagaccatc tcgcgcagca tcggggcgtt gccctgcatc gtcagggcat
     1681 gtccgatgcc ctcgtaggcg aggtcgatgt cgcgcttcag cgcgagcggg gcgagttcca
     1741 tcgccacctc cttcaccagg ccggcgaggt cgagcgcggc gcgttcgccg atcaggccgt
     1801 tgaccgcttc ggcgcgcgac agggtcagca tctggttggc cagccggcgc gccgcgcggg
     1861 tgctgcgcag caggctggcg aaggtcgcca gcatctcctc ggggtcggtc tgacgcagcc
     1921 cgtattcggc ctgcgtggtg agcaccgcga gcggggtgcg gatctggtgc gcggcgtcgg
     1981 cgaggaagcg ccggcgcgcg gcgagcatcg ccgaaaggcg ttccatgtgg tggttgatcg
     2041 cgtcgatcag cggcgcgatc tcgctcggca cgcgttccat gcgcaccggg gtgagatcct
     2101 cctcgtcgcg cgcgcgcatg tggtcgcgca cgcgcagcag ggggcggaag gcggtgccga
     2161 gcgcggccag caccaggatc gcccccaccg cgaccagcag cgactggcgc tgcaggctgg
     2221 cgacgaacag gtcgtgcgcg agcgcccggc gtgcaccggt ggtctcggcg aacacgatca
     2281 gcaccgcgtc gccgccgcgc agcgcggggt cgtagagacg cttgcgcatc gccgccaggc
     2341 gcaccgtctc gccggcatgg gtgaggtcga agatgcgtac gctgccgtcg tccgcgggca
     2401 gctccggtgg gcgcaggtcg gcatacccgg tgaccagccc gccggcaggg tcgaatacgg
     2461 catggaagat gcgttcggca gcatcggcct cgaagccttc gaaggcggcg taggggatgt
     2521 ccacctggat ctggccgccg agcgagtggg tgcgttcgcc gatcgcgcgc accgaggcgg
     2581 tgagcgtgcg gtcgtaggcc tggtttaccg cgtcgagcgc gctgcgatgg gtgaagaagg
     2641 cgttgatcag gagcagcacg gccaggggcg gcagcagcca gacgagcacg cgctgacgca
     2701 ggctgccgcc gtgtccgggc gcagctaccg ccctggcagc cgcgccgtgc tcgcgatcgt
     2761 ccgtgcccgg gtttgccggt ctggacccgg aggtgccgtc catgtcaggc tgccggcttc
     2821 tcggcgagca ggtagccgag cccgcgcagc gtgttgatcg ccacgcccgc gccttcgagc
     2881 ttcttgcgca ggcgcgagac gtagatctcg atcgcctcca cgcgtgcgtc ggcatcgaac
     2941 tcgaacacct tctcgaacag ggcctcgcgg gcgaccgggc gaccgacccg tgcgagcagc
     3001 gcttccagca cggccagctc gcgcggagtc agtgccagcg cctgtccgtc caccgccgcc
     3061 atgcgggtgg tggtgtcgaa ctcgagccgg cccaggcgca ccagcggcac ctggttgccg
     3121 gcgcggcgca gcagcgcctt gatgcgtgcc tcgagttcgg cgagctcgaa gggtttgggc
     3181 agataatcgt cggcgccgag gttgagcccg tgcacccgct cctcggtgct gccgcgcgcg
     3241 gtgagcacca gcaccggtgt gggagcaggc ttgtcggggc cgctctcgcg gttgcgcagg
     3301 cggcgcagca cttcgaggcc gtccagcccc ggcagggaca ggtcgaggat caccaggtcg
     3361 tagcgttccg tgcgcaagga ctgatcggca tggtcgccac gattgagcac atccagcacg
     3421 aaaccggcat gccgaagcga gcgcgccacc cattcggcga gttcggcgtg gtcctcgacc
     3481 agcaacaggc gcatcggtgt tcccgctgaa agtgaatcga aaggcgtgtg cgggattatc
     3541 ggcgcaaagc cggggtgcgt ccaaggcggc acatgccggg cattccccgc atggcggtgc
     3601 gggcgcgggg gcacgcggag cacgaggagg aggggtgatg aggatcaggg gtgggagggc
     3661 cgcgctgaag acggtgctgg cggtcgtggc ggcggtggcg atcgccccgg tgcgggccga
     3721 gccgatcgcc gatcgcttcg atcaggcacg cctggagggc gtggtggtcg tctatgcggc
     3781 gaccgacctc gaggtggtca agccggtcat cgacgacttc gaggccctcc atcccggcgt
     3841 tcgggtgcag taccacgaca tgcactcggc cgaactccat gcgcgcgtgg tcgacgaggc
     3901 ccggcgcggg ctggccggtg ccgacgtggt gtggagctcg gcgatggacc tgcaggtgaa
     3961 gctggtcaac gacggccacg cccagccgca ccgctccgcc gagaccgcgg cgctgccgcg
     4021 ctgggcggtg tggaaggacg aggccttcgg caccacctac gagccggcgg tgatcgtcta
     4081 caacaagcat ctgctcggca cgaccgaggt gcccgacagc catgcggagc tgatccgcct
     4141 gctcgatcgc gacccggcgc cgttgcgcgg gcgcatcgcc acctacgacc ccgagcgctc
     4201 cggcctcggc ctgctgctgc acacgcagga cgcgcaggcc aacccgatcg tgttctggca
     4261 gctcgcgcgc ggcatgggcc ggcagggcct ggagcagcac gcggcgagca gcgagatgct
     4321 cgaccgcgtc gccgcgggca agctggtgct cgcctacaac gtgctgggct cgtacgcgca
     4381 ccggcgggcg cgcagcgatc cggcgctcgg ggtggcgctg ccgcgggact acacgctggt
     4441 gctgagccgg gtcgccttca tcgtgcgtgg cgcgcgtcat ccggcggcgg cgcgcctgtg
     4501 gctcgatcat ctgctgtcga cccgaggcca ggccctgctc gccgccaacc tcggcctgct
     4561 gccggtgcgc accgacgccg gcaccgcggg cgcggacagc gctgccgcgc tgctccacca
     4621 caacctgcag catgccttcc gcccgatccg catcggctcc gggctgctcg cctaccagga
     4681 ccaggccaag aagcaggcct tcctgcgcca gtgggatgcg gcgacgcggc cggcttccga
     4741 gtgaggcccg cccggccgac cggatccccg gccctctccc ccaccgccgc gcagcgtggt
     4801 cccggatcgc ggtgcgtccc cagcggccct ttctccgttt cgtcccgatc tctcgccgat
     4861 ctcctgccga tgtccgccct ctccacgctc cgtctcgccg cctggcgcca gatccttccc
     4921 acgctcgcgc tcgccacgct cgccggctgg ctggccgaac gcctgcacct gccgctgccg
     4981 tggatgatcg gcccgctgtt cgccgtggcc ggcgcacgca tggccgaccg ggacctgcgc
     5041 ccgctccccg gtggacggca gacgggacaa tgggcgatcg gcgccgcgct cgggctgtac
     5101 ttcagtccgg aggtggtggc gcaactgagc ctgcatgcgc cgctggtgat cgcggccgcg
     5161 ctgggctcgc tggtgctcgg catggcctac gccttcattc tgctgcggct ggggggcgtg
     5221 gatgcgccga ccgcgttctt cgccgccctg ccgggcgggg cctcggagat ggccaacctc
     5281 gccgatcgcc acggcggtgc ggtggaccgc atcgccgcgg cgcacgccat ccgggtcatg
     5341 atggtggtgt cggtgctgcc cttcgcgctc accttcgccg gggtgcaggg cagcgacgtc
     5401 tacgtgcccc tggcgcggga tgtggacgcg cagcacttcc cgctgctggt gggcgcgagc
     5461 ctgctcgggg tgggggcgtt caagctgctg cgcatcgcca atgcgtgggt gctcgggccg
     5521 ctgctcggcg tggcgctggc gacgctggcc ggcgtgccgc tgtcggcgct gccgccctgg
     5581 gtcgtcaatg gcggtcagct gctgatcggc tgtgccctcg gctgtcgctt ctcgcgtgcc
     5641 ttcttccgcg ccgcgccacg cttcatgagc gtcgcggcgc tcaccggggc gatgtcgatc
     5701 gtgcttgcgt tcgccctcgc gaccctgctc ggcctggtcg cggaggtgcc gctgtcgacg
     5761 ctggcgctgg cgaccgctcc cggcggagtg tcggagatgt gcatcaccgc caaggtgctg
     5821 cagctgggcg tacccctggt caccgtgtgc catgtgttgc gcgtggtggt gttgaccgtg
     5881 ggtgcgcagt ggagcttcgg catcttccgc cgcctggtgg ccgtctgagc ggcgccggta
     5941 cgcccgcgcg ggcgtaccgg cgcgggatca ggcgccgtcc gccagcgtcg ggtaatcggt
     6001 gtatcccttc ggcccggggg tgtagaaggt cgccggatcg ggcgcattga gcgcggcctt
     6061 gcggcggatg cgttcgggca ggtcggggtt ggcgaggaag gcggtgccga aggcgaccgc
     6121 gtcgagcttg ccggcggcga tcgcggcgtt cgcttcgtcc gccgagtagc ccatgttgcc
     6181 gatcagcacg ccacggtagg cggcgcgcac cggtgtcacc acgtcgccgt gctgctggcc
     6241 gaagaaatcg ccgcgcatca ggtgcaggta ggcgagccgc cagtcgttca ggcagccggc
     6301 gagccagctt gccaacccga tcgggtcgct gtcgatcatg tcgttgtagc tgttgagcgg
     6361 cgacaggcgc acgccgacgc ggccgccgcc ccacaccttg cacaccgcgt cgagcacctc
     6421 gaagagcagg cgggcgcggt tctcgcgcgg tccgccgtag ggaccgccgc gctggttgct
     6481 gccgtcgcgc aggaactcgt cgagcaggta gccgttggcg ccgtgcactt ccacgccgtc
     6541 gaagccggcg gcacgggcgt tctcggcggc gcgcgcgaag ccggcgacga tgtccggcag
     6601 ctcgtcgtcg cgcagttcgc gcggcacgac gtagggctgc ttgccctggg gcgtgtgcac
     6661 ctcgtcgccg gtgatcgcga gcgcgctggg cgcaaccggc tggcggccgt cgttgagcag
     6721 cgggtggcag gcgcggccgc cgtgccagat ctgcaatacg atgcgcccgc cggcggcatg
     6781 caccgcgtcg gtgaccttct tccagcctgc gacctgggcc tcggagtgga tgccgggctc
     6841 gtgccagaag gcggagttgc cctccatcgc catcgtcgcc tcggcgatca gcaggccggc
     6901 gctggcgcgc tgggcgtagt gggtggccat cagcgcgccg ggcacgtggc cgtcctcggc
     6961 gcggcagcgc gtgagcgggg ccatcaggac gcggttgggc agggcgagat cgcccgcctg
     7021 cagcggggtg aagagatcgg tcatggggtc ttgtcctcgg ggtcgatcgg atcgaaaagt
     7081 cgggcgaagg ctgcgcgcgc cgccgtctcg gcgaggtcgg cgagacgcac ggcctgcgcc
     7141 ttgcgctcgg cgggcgcttc gtccagcacg gagcgcgcga gcgcgcggag gttccccgac
     7201 gcagccaggt gatggcgtcg tggcgggtgc caggtgtcga gccaggcgcg cagcttcgcg
     7261 gcctgcggtg ccccgcctgg aaggaggccg atcgcgggac gcgcgaaggc gtcggcgacg
     7321 atgcgtgcgt gcaggctgct cgcgcagcat agcgcggcgt tggcgatcaa cgcgcacaga
     7381 tccatgacgt ggggggaggc ggcgatgacg gccgcctccg gatcgggcag gcggcggcgc
     7441 aggcgctgca gtacgtcggg gtcgtcgtgc cagggcgcac gcccggcgca gaacagcacg
     7501 atgcccaggt cgcgctcgcg ctggatcgcc gcaagctcgc ccgcgagcgt ggcgagggtg
     7561 gcgtcgtcgc cgaactctgc cgcgagctgc accgccacgt agcctcgcgc gaagcgtgcc
     7621 cgcagcgccg cgacctcgcc cgtcgcggcg tggtgcgcca cctcggcgcc gagcaggcgt
     7681 tcgaccagca ccgcggggtc ggggaccagc gccgcgtcga ggcccgcggc ggcgagggcg
     7741 tcgcgggtgc agcggtcgcg cacctgcacc cctgcggccg cgcgcagctc ggcaagcgcc
     7801 tcggcacgca gcgccgcggg cagggccgcg aagccgaccc cgccgatgcc ggtgtgcacg
     7861 ctgcgcgtgc cgggcggcag cgcggcatgc gcagcgaggt agggcagacc acgcggaacc
     7921 ccgagcgcgt gccgtgccca ggccgcgcgc gcggcggggt cgtggtcgta tagcgcgatc
     7981 gcgcgtgcgg cgccggcgtt gtcgagcagc atcaccgccg cctcccaggc gctgcagtcg
     8041 aggatctcgc cacccacctg cagcagcccc ggcgggggcg tgtcgcgacg gggcggcttc
     8101 cattccgcgg cgagttcggc gagcgcttcc acgcgggcac cgccgtccgc acgcagatcg
     8161 cgcacggcga ggccggcgaa gcgcagctcc cgtccgggca ggaggcgcgc ggccagttcc
     8221 gctgcgatgc ggccgagcag caggtcgccg aggttgtggc ggtcgaaggc accgaacagg
     8281 atcagcggcg agggctgccc gacccggggc gtggcgccgg ccgccgcctg cgcgggcgag
     8341 agggggcgga gggatgtcgc ggtgcgcgcg ccgggcccgt tcatcgcccc ccctgcctca
     8401 atccgcgccg gtgcgcaggc ggtcgaggcg caggcgctgg cgctcgtcga agagccggcg
     8461 cgcgcccagg ctcaccgcca ccacggtacc gaagccggtg ccaccggcga tcaggaacat
     8521 caccaggatc tggtatttca ccgcctccac cggcgcggtg ccggcgagga tctggccggt
     8581 catcatgccg ggcaggctga cgatgccggc ggcggccatg gcgttgatga tcgggatcat
     8641 gccgacgcgg atcgcctcgc gccgggtgtc ggcgatggtc tcgttccagt tctggccgag
     8701 ggcgagccgg gtctcgagca ccgtgcgccg cgcgctcgcc tcctgggtca ggcggtccag
     8761 cccgagggcg atgccgttca tggtgttgcc gagcaccatg ccgagcagcg ggatcgcgta
     8821 ctggggcgca taccacggcg tgttgccgat caccaccagc agcgccagca cgctgacggt
     8881 gaaggaggac acgaacatcg acagcgtgcc caggccgaag ccccaccacc cgacgaagcg
     8941 gcgcttctgc cgcgccatca cctcgcgccc ggcggcggtg atcatcaccg cggccatcgc
     9001 cgccacccac aggaagtgcg cgttgtcgaa cagcagcttc agcaccagac cgatcagcag
     9061 caactggatc gtggtgcgcg cggccgccac caccaggcgc tgctcgatgc ccaggcccag
     9121 gcgcagcgac agcccggcca gcgccaggat cagcagcgcg gcgagcgcaa ggtcgaggac
     9181 cgagagctcc atcaccatgc cgcgccctcg gcgtcggggg gcctgtccgc gcctgcagcc
     9241 tttcctttgt ccgcggccgc cttcccgcct tcctcttcct ccaccagctt gccgtcctcg
     9301 agcaggaagc ggcgccgcgc cacccgtgcg gcctggcgcg ggtcgtggct gacccacagc
     9361 agcgcggcgg cgtgctcgcg ccggtagtcg tcgagcacgg cctcgacgcg cgcggtgctg
     9421 tcctcgtcga ggttgccggt gggttcgtcg agcagcagca ccgccggccc ctgcatcagc
     9481 gtgcgcagca gtgccaggcg ctggcgctcg cccgtggagc agcgcgccac ctgccagtcg
     9541 agcgccccgg cgggcaggtc cacgcggtcc agccaggcgg gcgtgcagcc ggcgggaaag
     9601 tgctcgccga ccgtatcggc ccaccactgg ccctcggcca tcaccagcgc cacccggcgc
     9661 cgccaggccg ggcccggcat ggacgagcac gcgcgcccat ccagcagggc ctcgcccgcg
     9721 tgcgggtcga gatcggcgat cgcgcgcagc agcagggact tgccgctgcc cgaggcgccg
     9781 cggatgcaga cacattcgcc ggcggcgacg tcgagatcga ccgggccgat gtggcgggtg
     9841 cggagctggc gcagggcgag ggcgggcatg gacggatgtt cgcgtgtccg ggcggggttt
     9901 tcaatgcggg tggctcgccg cgctcggcaa gcgcgctccg attgggcgag aatgtgcaag
     9961 caatccatgc acatcttcgc ctgcatggaa tcttttttcc gccaggggct cacacccccg
    10021 catgcggcgc ggacggtccg cgccgcccga caggaccgac tcatgtacag ggatccccga
    10081 ttctccggca tcttccagac cctcggcggg ccggccgcca agccgcccac gctgctgcag
    10141 aaggtggtgg gcgcggtcgt catggcgggc gtcttcatgc tcgcgctgac cttctcggtc
    10201 gccttcttcg cggtgctgct gaccgccggt gtggcgatct ggggctgggt gtggtggaag
    10261 acgcgtgccc tgcgcaaggc gatgcgggcg cagatggacg cgcaggcggc cgcaggcggt
    10321 gctcgtgcgg gcggctacgg tcgggccgct ggcgaccgtg ggctgatcat cgagggcgag
    10381 gtgattcgcg aggtggagat cgaggaggat ccgcgcaggg acggccccgg cggccgcacc
    10441 ccgtcgtgac gccagtcgcc ccacgcccgg cgcagccgcg ttcccagcta cccttgttga
    10501 cccggtgatc cggcgggcgg aggcaccgcc gtgcgctgca gcaaggcctc gcggccgtcg
    10561 gcgatgctgc ggatgtgcac atccatctgg ttgaaggcga tctcgattcc cgcttcacgg
    10621 aagcggcggt cgatctcgcg gttgatctcg tcgatcgcgg cgttgcggtc gccgagttcg
    10681 cgcacgtgca cccggagctc gtggtcgagc gtgctggggc cgaagttgag gaagaacacc
    10741 agcggttccg gatccttcag cacgcgcgcg ttttcggccg cgatcgcgag cagcaggcgc
    10801 cgcgtcgtct ccaggtcggc gccgtaggcc acgccgagct tgatcgtcac ccgcgtcacc
    10861 gtgtcggtca gcgaccagtt ggtgagctgg ccggtgatga aggccttgtt gggcacgatc
    10921 atctccttgc ggtcgaagtc ggtgatcgtg gtggcgcgga tctggatgcg gctcaccgtc
    10981 cccgagacgt tgccgatcgt caccacgtcg ccgatgcgca gcgggcgctc gaacaggatg
    11041 atcagaccgg agacgaagtt ggcgaagatc tcctgcaggc cgaagcccag gcccaccgac
    11101 agcgccgcca ccagccactg cagcttgtcc cagctcaccc ccagggtgcc cagcgtggaa
    11161 gcgatgccga cgccgacgat gacgtaggag agcagcgtgg tgatcgcata cgcgctgccc
    11221 tgggcgagct ggagccgcga cagcaccagc acctcgagca ggcccggcag gttggtggcg
    11281 agcatgaagg cgatcgccgc gatcaccagc gcgccgagca ggtcggcgag actgatcggc
    11341 accacgccgg cggtctcgcc actgccgctg gcgtactcgt acagggtgat gttgtcgagg
    11401 taggacacca ccgtcagcag gtcggcccac acccagtaaa gcgccaccgc gaggccggac
    11461 agcagggcca ggtggagcag gcgcagcgac tgctggttga tgcgggcgat gtcgagcatc
    11521 ggcgcctcgg ccaccgcagc cggctcgctg tcggcgccgg cgcggccctc gcgctcggcg
    11581 gcctcgcggc gcgcgaccgc gcgctgatgg tcgatgcggt gcgcggcgac gccgagcgcg
    11641 cgcaccagca ggccttcggc gagtcgccac aggatcagca cgtacagggt gtccaccagg
    11701 cggccggtga gcttgagcgc ggtgtagtag tagcccatca ccagcgcgcc gatcagcgcg
    11761 agcggcatga ggccgagcgc cagccccacg aacacgcgca gcggggaggg gcggtcttcc
    11821 aggcgcgtgt gcagcaggcc gcgcaccagc agccaggcca tggtggcgta gccgacgagc
    11881 aggatcagga tgcccagcac gtcgatgccc aggctctcgg gctggcgctc cgcgatcgcc
    11941 accaccgcga ccaccgccac caccaccaga ccgagcttgt tcaggttgcg gtgcagtgcg
    12001 cgcacgcgct cgacgggcca ctggaagtgg cgttcggcca ccccgcccgg ggtgaggagg
    12061 cgatgggcgg tgcggaacac cagccaggcc tgtgccatgt ccatcagcgc cgcgcccagg
    12121 gccgcgttct ggccgcgcgc gtccatctgc agggcgatgc cgccgagcgc cagcagcagg
    12181 cttaccggca aggtcagcag catgttgagc aggatcgcgc gcggggtgtg gagctggctg
    12241 tcgcggcgca cgtagccgat gtccgcatgc aggctgtcga gctgcgcgcg cagccagcgc
    12301 cggcgcgcga acaggccgac gctcaacagc agcaccggca ggaacaggaa gggccgcgcc
    12361 tgcaggcccg cccacagctc gccgacggcc gagccccacg ggatcgcggc gaactgcttg
    12421 gcgagcgcgg ccggcaccgc ctgcagccag gcgagatcga gcgggcggtt gctcgggatc
    12481 cagaacatct gcttgtcgag cgtggcgcgc aggcggtcgg cgctcaccag cagctggcgc
    12541 tcggcgagct gcagcgtgat cgactgggcg agcaccgcgt tgagctcgcg gttgaggcgc
    12601 tcgagcagct cgctgcgggt ggcgagcagc gccagcagcg gttcgcgcac ggccgggtcg
    12661 gcctcgccgg cgtcggcgag caggcgttcg gcgtgggcgg ccgggatgga gagctgttcg
    12721 cgctgcttgt tgatctcgaa ctggtacagg cggatgtcgg cgatctggtc ggcgaggccg
    12781 gcgtcggtcg tcggggcggg cagcgcccgg cgctgctcga gcaggatgcg cgagagcagg
    12841 acgctgccct gaagcacgcc gatctgctcg tcgatcagcg tggtgctgcg gttgagcacg
    12901 tcgagttgct ggcttaccgc ctggttgcgc cgggtgaggg cgtcgagctt ctcggtggcg
    12961 cccagcaggt attcggacag ctgctggttg atcgccgctt cgcgcgcgag gaggtcgtcg
    13021 tggctcgcgt cggcacttac gtcggcgagc gcctgcacgg tctgctcggc ctggctggcg
    13081 cgctggcggc tgatcgcgtc ctgcagcgcg cgtgccgcct cctcgaggcg ggcgagctgc
    13141 aggcgggcga gctcctggcg ggcgaggccg aggtcgatca ggccgctgtt gccggcgagc
    13201 tcctggcggc gcagcgcgag ctgggcgtcg agcgcatgcc attcggcggc gagcgcgtcg
    13261 cggcgctccg ggctgagcgc gcggctgtcg cgcccggctt cgcgtccgct cgcgagcatg
    13321 gcctcgatcg ccgccatgcg cacctggccg gtgctgatct cggtctgtgc gcgctcgggg
    13381 ccggtgcgcg cggcgacgag caggctgtcg gcctcgtcga ggcggcggcg ccagtcggcg
    13441 agcgcggcct cctgttcggc gaggcgctcc tcgaggcggg cgagcggggt gtcggcggcg
    13501 ggcgccgcga ccggcgcggc cgcaggcagc gcggcgagct ggcgacgcgc ctcggcgatc
    13561 tgtgccggtg cgtcggcgag tgtctgcttg agcgaatcga gccggcggcg atggtcgtcg
    13621 gcgctggcga ggctggcctg ggcctgttcc agcgcctgct gcgcggcgcg ttgctcggcc
    13681 tcgggcagct tgcggtccgc gagcgcctgc gcgcgcgcgc gcagctcgtc cggctgcggc
    13741 cgtggcggcg cttcggcggc ggcccagggg gccagcagcg taaacaggag cagcaggaag
    13801 actcggatca gcgggcgcat cgggaggatt ctgtggacgt ggcaggagat gggcctgcgg
    13861 gaggcgtggg gcggatcggc gtgcacggca gcaccgggtc caggggccga agtttagccg
    13921 gtcgccagga gccgggggcg cgtggccgga tgaaagcttc gtgtcgaaac atttgcttga
    13981 atggatacga ctccccgtct atcgtgtcgc gtccggagga gatggaaaac gacaagatgg
    14041 actaccagat ccagcgtctg aaggtgctcg gtgcgggcat cttcagcctg atgctcgcgc
    14101 tcggcgtggc gcgcttcgcc tataccccgc tgctgccgct gatgcaggcc caggccgggc
    14161 tcgggttggc cgagggcggc tggctcgcgg cgatcaatta caccggttac ctgagcggcg
    14221 cgctgctcgc cgcctcgatc agcgacctcg tgctcaagga ccgcctctac cgcatcggca
    14281 tggtggtcgc ggtgctgacc acgctgatga tggggctcac caccgatttc acggtgtggg
    14341 cggtgtcgcg ctacctggcc ggactttcga gtgcggcggc gatgttgctc ggcacgggcc
    14401 tgatcctgaa ctggctgatc cgtcacaacc atcgccacga gctcggcatc cacttcgccg
    14461 gcatcggtct gggcatcgcc ggctgctcgg tggccgtggc gctgatgagc ctgtggctgg
    14521 actggcgtgc gcagtggttc gtgttcaccg cgattgcctg cgtgctgctg gtgccggcgc
    14581 tgcgctggct gccggcaccc gacaccagcg ggctgacgcg cagcggcgcg ccgatgcacg
    14641 acgacccgcc cagcccgctc ttcctgcgca tcttcatggc gtcctacttc tgtgccggcg
    14701 tcggcttcgt ggtcagcgcg accttcatcg tcgccatcgt caatcgcctg cccgggctgg
    14761 agggccaggg gagctggagc tttctcgcca tcggcctggc ggcgatgccg gcctgcatcg
    14821 tgtgggattt catcgcccgc cgtaccggcg cgctgaacgc gctgatcctc gccgcggtgc
    14881 tgcagatcgt cggcatcctg ctgccggtgg tggtcggtgg cagcctgggg gcgatcgccg
    14941 gcgccctgct cttcggcggc accttcgtcg gcatggtcag cctggtgctg accatggccg
    15001 ggcgttacta cccgacgcgg ccggccaaga tgatgggcaa gatgaccatc tcctacggcg
    15061 tcgcgcagat cctcggcccg gcggtgaccg gctggctggg cgagaccttc ggcagctacg
    15121 caggcgggct gtggttcgcc gcggcgatga tgggcgtggg caccgtgttg ctggtgctgc
    15181 tgaagatcgt ggaccggcgc gacgctcagg ccgcggcagg cgtcgcctga gcgggcggcc
    15241 acggtcgccc gtggcgcagg cagaagtcgc gcagcagctg gttggccatc gcgtagcgga
    15301 tcgggaacag cggcggcagc gcgtcgacat ggaagaagcg cgcatcctcg atctcgcccg
    15361 gagcgcagac gatgtccccg ccggcgtact cggcctggta gccgagcatc agcgcatgcg
    15421 ggaagggcca cgcctggctg gagaaatagc gtggcgcgcg gatgcgcagg ccggtctcct
    15481 cgaagatctc gcgatgcacc gcctgctcgg ccgattcgcc cgcgtccacg aagccggcga
    15541 gcgtgctgta gaggccgggc gggaagtgcg gcgagcgccc gagcaggatc tcggcaccgc
    15601 gctcgaccgc cacgatcacc accggcgaga cgcgcggaaa gtgctcgcga ccgcagtccg
    15661 gcgcgctgca gcgccgcgcc ggcggatccg tccgcagcgt ggtgggcgcg ccgcaggcgc
    15721 cgcagaagcg gtgcgcgcgg tcgaactcga gcagctcgag ggcgcgcgcg gcgagggcga
    15781 gcgtctcccc gtcaagcgtg tcgagcagcg acttgagatg ctggaacgca tgtccgtcgg
    15841 gtgcctcgat cgtgcgctcg acctcgacca ccgtccatgc ctcgcccgtg gtcacgggga
    15901 agtcgagggt gcgcagcgcg acgagaccgg cccgccagga atccgggccg gccgtgggcg
    15961 tcgccgcgag gtccacgagc aactggtggc cgcggaacac gagcgtgcgt gccggccgcg
    16021 gatcgccgga tgggggcagt gaagaaggaa ggctcatgat cgtgggaccg gggtcgggcg
    16081 gagcgtcggc gcaaggagga aggcgagggc ggcagcatcg ggcagatcgc ccgtgctgtc
    16141 gagtctgtgg cgcgggtcgt gaccgcgggg ctcgtttcca ggcccggcaa ggcttgccgc
    16201 ggtttatttc gcgatgtgaa agaaggtttc gatgcccgac aggtcgataa gtggtatttt
    16261 tccgggcgac tgcgatcgtg cggcggcctt gccgcgccgg cgacagtggg tcgccatcgg
    16321 gtagtcggga caagatctaa aacatatagg ggaaatcaat gtctgcaggc aaatccaaga
    16381 tcatctacac gctcacggac gaagcgccgc tgctggcgac gtgcgccttc ctcccgatca
    16441 tccgcacctt caccggtcct gcaggcgtcg aggtcgagaa ggccgacatc tccgtgtcgg
    16501 cgcgcgtgct cgccgagttc cccgaatacc tgagcgaaga ccagcgcgtc cccgacacgc
    16561 tcgccgaact gggccgcctg accctcgagc ccgacaccaa catcatcaag ctgcccaaca
    16621 tcagcgcctc ggtcgcccag ctcaaggctt gcgtcaagga actgcaggag aagggctaca
    16681 agatccccga ctaccccgag gaccccaaga ccgacgagga aaaggcgctc cgcgtccgct
    16741 tcggcaagtg cctcggctcg gccgtcaacc cggtgctgcg cgagggcaac tccgaccgcc
    16801 gcgcgcccgc cgcagtcaag aactacgcca agaagcaccc gcactccatg ggcgagtgga
    16861 agcagtggtc gcagacccac gtctcccaca tgcaccacgg cgacttctac cacggcgaga
    16921 agtcgatgac gctcgaccgc gcgcgc
//
LOCUS       DAOQBS010000058        16819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2509_length_16819_cov_15.862622, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000058 DAOQBS010000000
VERSION     DAOQBS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 16819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16819
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2509_length_16819_cov_15.862622"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="PKN25_08270"
     CDS             <1..132
                     /locus_tag="PKN25_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing system ATP-binding protein
                     LolD"
                     /protein_id="HNV90932.1"
                     /translation="AVFDLMLSLNERLATSFVIVTHDETLAARAQRTLRLVDGRLGA"
     gene            239..2263
                     /locus_tag="PKN25_08275"
     CDS             239..2263
                     /locus_tag="PKN25_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV90933.1"
                     /translation="MKILFQPAVRLLDRLSYPLKFGLIILVCAVASVILLAQIFTSLR
                     EEIRVTEREIAGLQLFDAGFGVILKTQQHRGLSAGVLGGSSELAPKREAKAAELRAAL
                     GALDAAIDGDAGWSGLRAGWQMQRAALVRLADSGLSMAGAENFRLHTETIAGLMRWLG
                     ELGDASGLSLDPEPASSNLLAPLLGALPELSERLGQLRARGTALSARRELARSDEHAL
                     VALLAEIGRAESAVLERLRRTAAANASLAASVDGMGREISEGVARVREATGKELLDQR
                     FGLSSAAFFELVTGAIDTAVRNFDQVLRPEVGRLLEARRDRLNQRLQTQVAISAVGML
                     LAGYLFIGIYLAIVRSVRELSAGAQGFAAGDYRSRVQFSARDELAEVARQFNSMADHV
                     AGLIRDIQTGAAHVGGAATEMSASARRVLEGSETQSDAASSVAAAVEEMTRGVDGIAH
                     HASTAQQLAEDSGRLSQEGRSVMEQSVAEMERIAEAVDLSSEAIRELGEKSRQINAIV
                     DSIRDIADQTNLLALNAAIEAARAGETGRGFAVVADEVRKLAERTSLATREIGGMVAA
                     IQQGTERAVDTMQQGVQRVRDGVEMSNRAGSSMAQISAGADQVLTAVREISTALHEQS
                     LASNDIARNIERIADMTEHNSVTVRETAATAETLEQLAGTLREQASRFRV"
     gene            complement(2387..2923)
                     /locus_tag="PKN25_08280"
     CDS             complement(2387..2923)
                     /locus_tag="PKN25_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNV90934.1"
                     /translation="MRKLFKRFLPDHRSVSENRWLRPFASTLLHPRLWHLNRHSAAGA
                     VAVGMFCGLIPGPFQMPSAALFCVLFRVNLPLALITTLYSNPLTIVPLYVAAFAIGSL
                     MSGNGAVAFIPPPDFAGFDPLQWAADLAAWAGELGRPLVVGLIVLAASLSMVSYLAVR
                     WGWRLWLIRQWRQRRQRR"
     gene            3094..5541
                     /locus_tag="PKN25_08285"
     CDS             3094..5541
                     /locus_tag="PKN25_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNV90935.1"
                     /translation="MRIIALAFLAGTVLVQRSAALDGVVAAAALGLVAALGLRLALVL
                     AWGRGSAARAGRWIPIALAALGALAAGAGWAAWRAEVRLSDELAGTLDGVELQLRGRV
                     AGLPRASGSGWRFDFEVEPDTPGVPGLVQLAWRPERTVVEAKLDAEADSASPLPLAMP
                     RPGERWALTVRLWRPHGFANPSGFDYEAWLLERGVRATGSVRGTGRLLDELPATWMQH
                     VHRVRATVRERILAALPDGAHAGLLVALAVGDQQGIDAGQWEIFRRTGVAHLVSISGL
                     HVALVAMFCGGLAGAAWRRQPALVLRVPVQQVHALGGLAGATAYALLAGMGVPVLRAW
                     LMLLVGALALIGGRRIPPSRVLAIALLVVLVVDPWAVLSAGFWLSFGAVAVILAVLGG
                     RVAPSAGWRAALRVQLAISFALVPLLLAQFQAVPLLSPLANLVAIPLVSFVVTPLVLL
                     AIPWPIPLLLVPADFAAGWMMDFLAPLAALERAMIERAAPPPWLFGGALAAVAILLLP
                     RASPGRLAAPALLAALLSWQPARPQEGGFRAWVLDVGQGLAVHVQTRAHDLLYDSGPP
                     FGSESDAGERVVLPWLRAAGVRWLDRVVLSHPDSDHVGGAASLLAGLPVGTVLAGQAV
                     HARERAAWAARLAHGREPADCARVPAWERDGVRFEILHPAPVRAPSEEPAGAGASPHD
                     NDASCVLRVVGAAGALLLTGDIGTAAEAKLIARQPPASLSAAVVVSAHHGSRSSSSAA
                     FVDATLPEHAVHSAGSRNSFGHPHPEVWARWAEAGARNWRTDAQGAIRIDFAAAPEEG
                     VQVNAEREMQPRYWHGR"
     gene            5624..6595
                     /locus_tag="PKN25_08290"
     CDS             5624..6595
                     /locus_tag="PKN25_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV90936.1"
                     /translation="MSLLDSLKKFFSPSAPTTPAERAAVEPERVRAAQPLLQPRARHV
                     LCSGPHGLHRVAYTEWGEPTNRRVLVCVHGLTRNGRDFDDLARALAGEYRVVCPDVVG
                     RGRSDWLTDKADYGFPVYVADMVTLIARLDVEEVHWVGTSMGGIIGMLLASLAGTPIT
                     RLVLNDVGPVITAASLRRIGQYVGRAPRFADPAAAEAYIREVGAPFGALTDAQWRHLT
                     EYSVRRMDDAEGGYAMVYDPGLGEVFRLNPVPVDIDLWPVYEAIRCPTLALRGAESDL
                     LEPATFAAMGARGPRAQTVEFPGVGHAPMLMDAAQIATVREFLLAGA"
     gene            complement(6629..7573)
                     /gene="rsgA"
                     /locus_tag="PKN25_08295"
     CDS             complement(6629..7573)
                     /gene="rsgA"
                     /locus_tag="PKN25_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNV90937.1"
                     /translation="MRKRTHAGAIAEARIDGVVVAAFGRHYEVDTARGRLQCYPRGKK
                     SSYACGDEVELVPGGDGQGVIETLHPRRNLLWRSDAFREKLIASNLSHLVIVVATEPG
                     FSDLLVSRCIAAAESQGIVPLIVLNKADLAARLEAARHQLEPFRKLGYEVVEVSALQG
                     ADALRARLAGLQAIFVGQSGMGKSTLTNAIVPEAQAATREISDALDSGKHTTTFARLY
                     HLPVTADEARGGWLIDSPGLQVFGLAHLAAEDLAETFVELRPHLGRCRFRDCRHDSEP
                     GCALLGALEAGAIHPRRWEHFRAIRSEIAEARRLNPGW"
     gene            complement(7579..8865)
                     /locus_tag="PKN25_08300"
     CDS             complement(7579..8865)
                     /locus_tag="PKN25_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV90938.1"
                     /translation="MTDPAPFMLSPFGLPPLSVLFLAFLVAGTVLGLGLLHRHAHHVR
                     RHRDAVPQPFAGSIPLHSHQRAADYTVARARLSAFHAAANAGFVLALTLGGGLQAMHD
                     AWADVLPAGGLAHGVALLASLGVLGWLFELPFALLRTFGIEKTFGFNRMTPRLYLADT
                     VREAALAALIGLPLLAAVLWLTLATGALWWAWVWAFWLGFNLLAMVIWPTFIAPLFNK
                     FTPLADATLKARVEALLARCGFRAKGLFVMDGSRRSAHGNAYFTGLGAAKRIVFFDTL
                     LDKLDADEVEAVLAHELGHFHHRHLLRRLAVLAPASLGVLALLGWLAQQPWFFSGLGM
                     HSADLASALALFTLVLPVFSFPLAPLASHWSRKHEFEADAYAARQADAGKLVSALVKL
                     YRDNASTLTPDPLYSRFHDSHPPAALRIARLQALQR"
     gene            9051..9599
                     /gene="orn"
                     /locus_tag="PKN25_08305"
     CDS             9051..9599
                     /gene="orn"
                     /locus_tag="PKN25_08305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNV90939.1"
                     /translation="MAQDPKTLIWLDMEMTGLEPDRDRIIEIAIVLTDEALEVIAEAP
                     VIAVHQPDSVLDGMDEWNRNTHGKSGLTARVRASTMSEADAEAQVLAFLRAHVPARTS
                     PMCGNSVCQDRRFLARWMPELEAWFHYRNLDVSTLKELARRWRPEIYAGVKKAGSHTA
                     LADIHESIAELRHYRDHFLRLD"
     gene            9616..9777
                     /locus_tag="PKN25_08310"
     CDS             9616..9777
                     /locus_tag="PKN25_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90940.1"
                     /translation="MRIGPAGRVRGLVAWASRHRRWLIVLALLSVFFSVLGYFTNPVS
                     LPGMCRIYG"
     gene            complement(10026..11684)
                     /locus_tag="PKN25_08315"
     CDS             complement(10026..11684)
                     /locus_tag="PKN25_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90941.1"
                     /translation="MIRDPATPTLFQRRVYGTVGLTLLVGLYLLVGLTAHDPWRGDDA
                     RHFGPVLAMLRGESWLFPLLAGEPLVEHPPLYYWVASAFSALTAAFLPAHAGARLASA
                     FFVALAIWWLARAAESLHGRAARTPAALLALGTLGLVLHAHETQPLLALFAMLALTLS
                     GLAELPTRPLAGGLKAGMGSGLAFLSAGFGGLWMSAPLLPLAMLLSADCRSPRASGAV
                     LLGLCLALTTAALWPLVLHLQHPELLSLWLRNEWQWIVGPMLRPAELPRLLELGAWFM
                     WPLWPLALWALWRARRQPGVLAILLPLASAGLVLAWLVAIDDLSQARLIPLVPALALL
                     AAGGVASLRRGAASALDWFSLMSLAVFGILVWLAWSAQVAGWPSGLARHVARNAPGFV
                     PPDANLRLLAGGAISVVWILLVARLPRSPARAPANWAIGLVMLWSLAVVLLMPWFDQG
                     RSYRTVVESLDIAVRGERGAQARECIATTGLPDAMKLSMDYFAGLRARPIDADATPCA
                     LLLVHVERRGRSAALAPEWETVWEYRRGAGKRAETFRLLRRAGR"
     gene            complement(11867..12085)
                     /gene="rpmE"
                     /locus_tag="PKN25_08320"
     CDS             complement(11867..12085)
                     /gene="rpmE"
                     /locus_tag="PKN25_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNV90942.1"
                     /translation="MKADIHPKYADVNVTCSCGNTFVTRSTLGKPQYHVEVCSACHPF
                     YTGKQKIVDTAGRVERFRQKYGNVQRAS"
     gene            12267..13277
                     /locus_tag="PKN25_08325"
     CDS             12267..13277
                     /locus_tag="PKN25_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNV90943.1"
                     /translation="MRLLLAPMEGVADFVLRDVLTAIGGYDLAVSEFVRVSGTLLPRR
                     MFERICPEILAGSRTAAGTPVAVQLLGSDPVLLAENAARLAALSPHGIDLNFGCPAKV
                     VNRHGGGAMLLDRPRLLHDIAAAVRAAVPAAIPVSAKMRLGIADPSRALDCAQALADG
                     GAQAIVVHARTREQGYRAPAHWEWVARIGDAVRVPVTANGEVWSVRDWARCREVSAAE
                     DVMIGRGAVSDPWLARRIRGERGTEPDERDWVELHPHLLRYWEGVQGRAAPVHACGRL
                     KLWLGMLRRNYPAAGRLHEAVRRVVHPSRMNEELRRHGIPACDHPPSARADGPRPTSP
                     DR"
     gene            13288..13965
                     /locus_tag="PKN25_08330"
     CDS             13288..13965
                     /locus_tag="PKN25_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV90944.1"
                     /translation="MSYANSRIPQSAQQGVHEALAERVRKHLEHPFRKPCTDYNRAAL
                     AEALAGWGRSAPLILDAGCGVGHSTIQIARQFPDHWVIGVDQSADRLARRKPYPEALL
                     PKNMVFVRADLVDFWRLLDEAGLRLARHYLLYPNPWPKIGHLGRRWHAHPVFPWIPRL
                     GGVLECRSNWKVYIEEFAFALGLVLGREVAWEGFEAATPLTPFERKYRDSGQPLYRIG
                     LDLAAAG"
     gene            14044..14808
                     /locus_tag="PKN25_08335"
     CDS             14044..14808
                     /locus_tag="PKN25_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNV90945.1"
                     /translation="MEQRGADASALRTMSDEDFEALEEILTSDIVPEDCMDLEMLDGY
                     LAGVMISPRPIPAERWMPGVWSAHGDEVGFGSGSGLQRAIRLVKAYHNELLATLGLDD
                     EDEACWEPFCFAVAEGDGLKLGEAWIDGFAQGLDLWPEGWEEGLDAEAVEAVRETLDE
                     VLAPWGEEDAAKADDETRLGWLADVGEAVNDIFAHWRDLGLPAAEPLSTEAPRKPASA
                     GPGRNDPCPCGSGKKYKKCCGANGAEPEPESGEAET"
     gene            15146..15505
                     /locus_tag="PKN25_08340"
     CDS             15146..15505
                     /locus_tag="PKN25_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV90946.1"
                     /translation="MDEFNPCLDCGICCTHFRISFYWAEADDAPGGFVPAEMTEKLNH
                     TLRCMKGSNQVPRRCSALSGEVGRQVACSIYENRPTPCREFPVYFEDGTPNPKCDELR
                     ATIGLPPLPQSPLPRAA"
     gene            complement(15528..16514)
                     /locus_tag="PKN25_08345"
     CDS             complement(15528..16514)
                     /locus_tag="PKN25_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HNV90947.1"
                     /translation="MTSTPTARDPASDAAPASASAPAHRSPSDAAALTAHPPSSARSR
                     ADDLLERTDHVIAAALASGALQPIRTELDWLEQDGLRFSVRWVSSLALKDRARVDTVT
                     ARRPDFNPFLPPEPALTVAALGADHLVVLNKFPVIDRHLLIVTRRFEDQSTPLGGADF
                     AALAAVVGAHGGLGFYNGGRIAGASQAHKHLQWVPAQASALEDFLPPLHPGAAAENLA
                     LPWAHAAVGVDESAWSHPDDAGSVLLAAYRLACERLELPCDADPMPPYNLLVSRGALL
                     LVPRTREKWDDVSLNSLAWAGSLFVRDRAQIDGLRRHGPLEVLAAVGRPRGG"
     gene            16594..16818
                     /locus_tag="PKN25_08350"
     CDS             16594..16818
                     /locus_tag="PKN25_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNV90948.1"
                     /translation="MAIRFEVRGEYIQLDQLLKAAGLVESGGAAHAAVENGLVKVDGK
                     PESRKRAKLRPGQRVSFAGEAVELVEEGAG"
BASE COUNT         2414 a         5820 c         6234 g         2351 t
ORIGIN      
        1 gcggtgttcg atctcatgct ttcgctcaac gagcgcctgg cgaccagctt cgtcatcgtc
       61 acccacgacg agaccctggc cgcgcgcgcg caacgcacgc tccgcctggt ggacggccgg
      121 ctcggcgctt gaggaaaaaa tcccgcggca acgccccggg atgggtcaag ttctaccgga
      181 aacgaccgta tacaggctcg aagaccatcc tgtccggccc cgcgccggga atcgagccat
      241 gaaaatcctc ttccagcctg ccgtccgcct gctcgaccgc ctgagctatc cgctcaagtt
      301 cgggctcatc atcctcgtct gtgccgtcgc ctcggtgatc ctgctcgcgc agatcttcac
      361 cagcctgcgc gaagagatcc gcgtgaccga acgcgagatc gccggcctgc agctgttcga
      421 cgcgggcttc ggcgtgatcc tcaagacgca gcagcaccgc ggcctgtccg ccggtgtgct
      481 ggggggcagc agcgagctgg cgcccaagcg cgaggcgaag gcggcggagt tgcgtgccgc
      541 gctcggcgca ctcgacgcgg caatcgacgg cgatgccggc tggtcgggct tgcgcgcggg
      601 ctggcagatg cagcgggcgg cgctggtacg cctcgccgac agcgggctct ccatggcagg
      661 cgcggagaat ttccgcctgc acaccgagac gatcgccgga ctcatgcgct ggctcggcga
      721 gctcggtgac gcctccgggc tgtcgctcga ccccgagccg gcgagttcca acctgctcgc
      781 gccgctgctt ggcgcgctgc ctgaactcag cgagcggctc ggtcaactgc gtgcccgtgg
      841 cacggcgctg agcgcccgcc gcgagcttgc gcgcagcgac gagcacgccc tggtcgcgct
      901 gctcgccgag atcggccgcg ccgagtctgc ggtgctggag cgcctgcgcc gcaccgcggc
      961 ggccaacgcc agcctcgccg ctagcgtcga cggcatgggc cgcgaaatct ccgagggtgt
     1021 cgcgcgggtg cgtgaggcta cggggaagga gcttctcgac cagcgcttcg gcctgagctc
     1081 ggcggccttc ttcgaactgg tcaccggcgc gatcgacacc gcggtgcgca acttcgacca
     1141 ggtgctgcgt ccggaggtcg gccgcctgct cgaggcgcgc cgcgaccgcc tgaaccagcg
     1201 cctgcagacc caggtggcga tttcggcggt cggcatgctg ctcgcaggct atctcttcat
     1261 cggcatctat ctcgccatcg tgcgctcggt gcgcgagctc tcggccggcg cgcagggctt
     1321 cgccgcgggc gactatcgca gccgcgtgca gttctcggcg cgcgacgagc tcgccgaggt
     1381 ggcgcgccag ttcaactcga tggccgatca cgtcgccggc ctcatccgcg acatccagac
     1441 cggtgccgcc cacgtcggcg gcgccgccac cgagatgtcg gcctccgcgc gtcgcgtgct
     1501 ggagggctcc gagacgcaga gcgacgctgc ctccagcgtg gccgcagcgg tcgaggagat
     1561 gacgcgcggg gtcgacggca tcgcccacca cgcctcgacc gcgcagcagc ttgccgagga
     1621 ttcgggccgg ctctcgcagg aggggcgctc ggtcatggag cagtcggtgg ccgagatgga
     1681 gcgcatcgcc gaggcggtgg atctgtcgag cgaggccatc cgcgagctcg gcgagaagtc
     1741 gcgccagatc aacgcgatcg tcgactcgat ccgcgacatc gccgaccaga ccaacctgct
     1801 cgcgctcaac gccgcgatcg aggccgcccg tgcgggcgag accgggcgcg gcttcgcggt
     1861 ggtggccgac gaggtgcgca agctcgccga gcgcacctcg ctcgccacgc gcgagatcgg
     1921 cggcatggtg gcggcgatcc agcagggcac cgagcgcgcg gtcgacacca tgcagcaggg
     1981 cgtgcagcgc gtgcgcgacg gcgtggagat gagcaaccgc gccggctcct cgatggcgca
     2041 gatcagcgcg ggcgccgacc aggtgctcac cgcggtgcgc gagatctcca ccgccctgca
     2101 cgagcagagc ctggcgagca acgacatcgc gcgcaacatc gagcgcatcg ccgacatgac
     2161 cgagcacaac agcgtgaccg tgcgcgagac cgcggccacc gccgagacgc tggagcagct
     2221 cgccgggacc ctgcgcgagc aggccagccg cttccgcgtc tgaagtgttc gcggtgcccg
     2281 ctgcgatcgc agcggtgccg cgatcgattc tccatgagcc ctgcggcgat gcgaaggccg
     2341 tgccgcgcgc agggctcgaa tcttgcggac cggacgctgc gcccgcttat cgccgttgcc
     2401 gtcgctgccg ccactggcgg atcagccaca gccgccagcc ccagcgcacc gccagatacg
     2461 acaccatcga caggcttgcc gcgagcacga tcaggccgac caccagcggg cggcccagct
     2521 cgcccgccca ggctgcgagg tcggccgccc actgcagtgg atcgaagccg gcgaagtccg
     2581 gcggcgggat gaacgcaact gcaccatttc ccgacatcag gctcccgatc gcgaatgccg
     2641 cgacgtagag ggggacgatg gtgagcgggt tgctgtagag cgtggtgatc agcgccagcg
     2701 gcaggttgac cctgaagagc acgcaaaaca gcgccgcgct cggcatctgg aaggggccgg
     2761 gaatgaggcc gcagaacatt cccaccgcca ccgccccggc cgccgaatgg cggttcaggt
     2821 gccacaggcg cggatgcagc aaggtggacg cgaacggccg cagccagcgg ttctcgctga
     2881 cgctgcggtg gtcggggagg aagcgtttga agagcttgcg catggttcag gtccgcgtgg
     2941 gcgagtctag caggagcccg gcccatcgcg tcgtggcatg cgcggccatc ctcgacgcgg
     3001 cggcccgggt cgcgatcgtc cgtcgagggc ggaagggcgg ccggcgatca cggcgcccgc
     3061 ccttccggga atgcctgagg catactcgat gccatgcgca tcatcgctct cgccttcctc
     3121 gccggcaccg tcctcgtcca gcggtcggct gcgctcgatg gcgtcgtggc cgcggcggcg
     3181 ctcggcctcg tcgctgcgct cggcttgcgg ctggcgttgg tgttggcgtg gggccgcggg
     3241 tcggcggctc gggctgggcg ctggatcccc atcgccctgg ccgcactcgg tgcgctcgcc
     3301 gccggcgcgg gctgggcggc gtggcgggcc gaggtccggc tgagcgacga gctcgccggc
     3361 accctcgacg gtgtcgagtt gcagctgcgg ggcagggtgg ccgggctgcc acgcgcgagc
     3421 gggtccggct ggcgcttcga cttcgaggtc gagcccgaca cgcccggcgt gccgggactc
     3481 gtgcagctgg cctggcgtcc cgaacgcacc gttgtcgagg ccaagctcga cgccgaggcc
     3541 gattccgcga gccccctgcc gctggcgatg ccccgtccgg gcgagcgctg ggcgctgacc
     3601 gtgcgtctgt ggcggcccca cggtttcgcc aatccctccg gcttcgacta cgaggcctgg
     3661 ttgctcgagc gcggggtccg cgccacgggc agcgtgcggg ggacggggcg gctcctagac
     3721 gagcttcccg ccacctggat gcagcacgtc catcgcgtgc gcgcgacggt gcgcgagcgc
     3781 atcctcgccg ccctgcccga cggggcgcac gccggcctgt tggtggcgct cgcggtcggc
     3841 gatcaacaag gcatcgatgc cgggcagtgg gagatcttcc gccgcaccgg cgtcgcccac
     3901 ctggtgtcca tctcgggcct gcacgtggcg ctggtggcga tgttctgcgg cggtctcgcc
     3961 ggcgcggcgt ggcgccggca gccggcgctg gtgctgcgtg ttccggtgca gcaggtgcac
     4021 gcgctcggcg ggctggccgg ggccaccgcc tatgccttgc tggccggcat gggcgttccg
     4081 gtgctgcgcg cctggctgat gctgctggtg ggcgcgctgg cgctgatcgg cgggcggcgc
     4141 ataccgccct cgcgcgtact cgcgatcgca ttgctcgtgg tgctggtggt cgatccctgg
     4201 gcggtgctgt cggccggctt ctggctgtcc tttggtgcgg tggcggtgat cctcgccgtg
     4261 ctcggcggcc gtgtcgcacc gtcggccggc tggcgcgccg cgctgcgcgt gcagctcgcg
     4321 atcagcttcg cgctggtgcc gctgctgctt gcgcagttcc aggcggttcc gttgctgtcg
     4381 ccgctcgcca acctggtggc gattccgctg gtgagcttcg tcgtcacgcc gctggtgctg
     4441 ctcgcgatcc cctggccgat ccccttgctg ctcgtgccgg ccgacttcgc cgcggggtgg
     4501 atgatggatt tccttgcgcc gcttgcagcg ctcgagaggg cgatgatcga gcgcgcggcg
     4561 ccgccgccgt ggcttttcgg cggtgcgctc gccgccgtgg cgatcctgct gctgccgcgc
     4621 gcaagcccgg ggcggttggc ggcaccggca ttgctcgcgg cgctgctgtc ctggcagccg
     4681 gcgcgcccgc aggagggcgg tttccgcgcc tgggtgctgg acgtcggcca gggtctcgcg
     4741 gtgcatgtgc agacgcgtgc gcatgacctc ctctacgaca gcgggccacc cttcggcagc
     4801 gagtccgatg ccggcgagcg ggtggtcctg ccctggctgc gcgcggccgg cgtgcgctgg
     4861 ctggatcggg tggtgctgtc gcatccggat tccgatcatg tgggcggcgc ggcctcgctg
     4921 ctcgccggcc tgccggtggg cacggtgctg gccggacagg ccgtccatgc gcgcgaacgc
     4981 gcagcctggg cggcccgcct ggcgcacggg cgcgagccgg cggactgcgc acgcgtgccc
     5041 gcatgggagc gcgatggcgt gcgcttcgag attctgcatc cggcgcccgt gcgcgcgccg
     5101 agcgaggagc cggcaggcgc gggcgcaagt ccgcacgaca acgacgcctc ctgcgtgctg
     5161 cgcgtcgttg gcgccgccgg cgcgctgctg ctgaccggcg acatcggcac cgccgccgag
     5221 gccaagctga tcgcccgcca gccaccggca agcctttccg ccgcggtcgt ggtcagcgcc
     5281 caccatggca gccgctcgtc gtcctcggcc gccttcgtcg acgcgaccct cccggaacac
     5341 gcggtccact ccgccggcag ccgcaacagc ttcggccatc cccaccccga ggtgtgggcg
     5401 cgctgggcgg aggcaggggc acgcaattgg cgcaccgatg cgcagggtgc gatccggatc
     5461 gacttcgcgg cggcgccgga ggagggcgtc caggtgaacg ccgagcgcga gatgcagccg
     5521 cgctactggc acggtcgctg agggcggcgg gcttgtgtcc cggatccgcc gcttcgggct
     5581 agacttgcag cgacccgaaa cccgttccga gtgcccgccc ctcatgtccc tgctcgattc
     5641 gctcaagaag ttcttcagcc cgtccgcacc gacgaccccg gccgagcgtg ccgcggtgga
     5701 gccggagcgt gtgcgcgccg cgcaaccgct gctccagccg cgcgcccggc acgtgctgtg
     5761 cagcgggccg cacggcctgc accgcgtggc ctacaccgaa tggggcgagc cgacgaatcg
     5821 gcgcgtgctg gtctgtgtcc acggtctcac ccgcaacggc cgcgacttcg acgatctcgc
     5881 gcgcgcgctc gccggcgagt accgcgtcgt ctgcccggac gtggtcgggc gcgggcgcag
     5941 cgactggctg acggacaagg cggactacgg cttcccggtc tacgtcgccg atatggtcac
     6001 cctgatcgcc cgcctcgacg tcgaggaggt gcactgggtc ggcacctcga tgggcggcat
     6061 catcggcatg ctgctcgcga gcctggccgg cacgccgatc acccggctgg tcttgaacga
     6121 cgtcggtccg gtgatcaccg cagcatccct gcgtcgcatc ggccagtacg tcggacgcgc
     6181 gccgcgcttt gccgaccccg cggcggccga ggcctacatc cgcgaggtcg gcgcgccctt
     6241 cggcgcgctc accgacgcgc agtggcgcca cctcacggaa tattcggtgc gtcgcatgga
     6301 tgacgccgaa gggggctacg cgatggtgta cgaccccgga ctgggcgaag tcttccggct
     6361 caatccggtg ccggtcgata tcgacctgtg gccggtatat gaagcgatcc gttgcccgac
     6421 cctggcgctg cgcggcgcag agtccgacct gctggagccg gccaccttcg cggcgatggg
     6481 cgcgcgcggg ccgagggcgc agacggtgga gttccccggc gtggggcacg cgccgatgct
     6541 gatggatgcg gcgcagatcg ccacggtgcg ggaatttctg ctcgccggcg cctgagttgc
     6601 gcgtgcaggc gcgtcgggcg ggcgtcgcct accagcccgg attgagccgt cgcgcctcgg
     6661 cgatctcgct gcggatggcg cggaaatgct cccagcgccg cgggtggatg gcgccggctt
     6721 cgagtgctcc gagcagcgcg caacccggct cgctgtcgtg gcggcagtca cggaagcggc
     6781 agcgccccag gtgcgggcgc agctcgacga aggtctcggc gaggtcctcc gccgccaggt
     6841 gggcgagccc gaacacctgc aggccggggc tgtcgatcag ccagccgccg cgggcttcgt
     6901 cggcggtcac cggcaggtgg tacagccgtg cgaaggtcgt ggtgtgcttg cccgagtcga
     6961 gcgcgtccga gatctcgcgg gtggcggcct gggcctccgg cacgatggcg ttggtcaggg
     7021 tcgatttgcc catgcccgac tggcccacga agatcgcctg caggccggcc aggcgagcgc
     7081 gcagcgcatc cgcgccctgc agcgcggaca cctcgacgac ctcgtagccg agcttgcgga
     7141 agggctcgag ctgatggcgg gcggcctcga ggcgcgcggc gaggtcggcc ttgttgagca
     7201 cgatcagcgg cacgatgccc tggctctcgg ccgcggcgat gcagcgcgac accagcaggt
     7261 cggagaaacc gggctcggtg gcgaccacga tcaccagatg gctgaggttg gaggcgatca
     7321 gcttctcgcg gaaggcatcc gagcgccaca gcaggttgcg gcgcggatgc agggtctcga
     7381 tcacgccctg gccgtcgccg ccgggcacga gctcgacctc gtcgccgcag gcgtagctgc
     7441 tcttcttgcc gcgcggatag cactgcaggc gcccgcgcgc ggtgtcgacc tcgtagtggc
     7501 ggccgaaggc ggccacgacc acgccgtcga tgcgggcttc ggcgatcgcg ccggcgtggg
     7561 tgcgcttgcg catgccgctc accgttgcag cgcctgcagg cgcgcgatgc gcagcgcggc
     7621 gggcggatgc gagtcgtgga agcgcgagta cagcgggtcg ggcgtcagcg tggaggcgtt
     7681 gtcgcggtag agcttgacca gcgcgctcac cagcttgccg gcgtcggcct ggcgggcggc
     7741 gtaggcgtcg gcctcgaact cgtgcttgcg cgaccagtgg ctcgcgagcg gcgcgagcgg
     7801 gaagctgaac accggcagta ccagcgtgaa cagggcgagc gcgctcgcca ggtcggcgct
     7861 gtgcatgccc agcccggaga agaaccaggg ctgctgggcg agccagccga gcaaggcgag
     7921 cacgcccagg ctggccgggg cgagcaccgc cagccggcgc agcaggtggc ggtggtggaa
     7981 gtggccgagc tcgtgcgcga gcaccgcctc gacctcgtcg gcatcgagct tgtcgagcag
     8041 ggtgtcgaag aacacgatgc gcttggccgc gcccagcccg gtgaagtagg cgttgccgtg
     8101 tgccgagcgg cgcgagccgt ccatcacgaa caggcccttg gcgcgaaagc cgcagcgcgc
     8161 gagcagggcc tcgacgcgcg ccttcagcgt ggcgtcggcg agcggggtga acttgttgaa
     8221 cagcggcgcg atgaaggtcg gccagatcac catcgcgagc aggttgaagc cgagccagaa
     8281 cgcccacacc caggcccacc acagcgcgcc cgtcgccagc gtcagccaca gcaccgcggc
     8341 gagcagcggc agcccgatca gcgcggcgag cgcggcctcg cgcacggtgt cggcgaggta
     8401 gaggcgcggc gtcatgcggt tgaagccgaa ggtcttctcg atgccgaagg tgcgcagcag
     8461 cgcgaagggt agttcgaaca gccagccgag cacgcccagg ctggcgagca gcgcgacgcc
     8521 gtgggcgagc ccgccggcgg gcagcacgtc cgcccaggcg tcgtgcatcg cctgcagccc
     8581 gccgccgagc gtgagcgcga gcacgaagcc ggcgttggcc gcggcgtgga aggcggacag
     8641 gcgcgcgcgc gcgacggtgt agtcggcggc acgctggtgc gaatgcaggg ggatcgagcc
     8701 ggcgaagggc tgcggcaccg cgtcgcgatg gcggcgcacg tggtgggcgt ggcgatgcag
     8761 cagaccgagg ccgagcacgg taccggcgac caggaaggcc aggaacagga cggaaagagg
     8821 cggcaggccg aagggcgaca gcatgaatgg ggcgggatcg gtcattgcga agcgcgggcg
     8881 gtggatggag gtagggctgc gggcaggtgt gcgacccgac cggccatgcg ctgctgtccg
     8941 tgacaggctg gcgcgatcgc tgctggccga ggccggcaac gtacgcgatc tatgacacaa
     9001 tcgcgccctc attgtttcgc aggaaggccc cggaaaaggg cggacggatt atggcacagg
     9061 acccgaagac gctgatatgg ctggacatgg agatgaccgg cctcgaaccc gatcgtgacc
     9121 gcatcatcga gatcgcgatc gtgctcaccg acgaggcgct cgaagtgatc gccgaggcgc
     9181 cggtgatcgc cgtgcatcag cccgacagcg tgctcgacgg catggacgag tggaaccgca
     9241 atacccatgg caagtccggg ctcaccgctc gggtacgcgc ctccacgatg tcggaggccg
     9301 atgccgaggc gcaggtgctg gccttcctgc gcgcgcacgt gcctgcgcgc acctcgccga
     9361 tgtgcggcaa ttcggtgtgc caggaccgca ggttcctcgc gcgctggatg ccggaactcg
     9421 aggcctggtt ccactaccgc aacctcgacg tgtccacgct caaggagctc gcgcggcgct
     9481 ggcgtccgga gatctacgcg ggtgtcaaga aggcgggcag ccacaccgcg ctcgccgaca
     9541 tccacgagtc gatcgccgag ctgcgccact atcgcgacca cttcctgcgc ctggactgag
     9601 cgcgaggagc gcgacatgag gatcggtccc gccgggcggg tccgcggcct cgtcgcctgg
     9661 gcgagccgtc accggcgctg gttgatcgtg ctcgcgctgc tgtcggtgtt cttcagcgtg
     9721 ctgggctatt tcaccaaccc ggtatcgctg ccggggatgt gcaggatcta cggctgaggg
     9781 ccgtgcgcag gcgctggccc tcgacgtccg tcgcagccga accgtccgat cgcgacttgc
     9841 gtcgctccca cgaaaaccga cagggtgccc ccggggtgac gcggcgccaa ggcgttacgg
     9901 gttccggaag gtgacggggg gaccggcgcg tgaagcggct tcgacgtcgg gtaggagcga
     9961 cgcaagtcgc gattcgggca ggcgagaatg caaggacctc gccgcaacgg cacggatgcg
    10021 tcgattcagc gtccggcgcg gcgcagcagc cggaaggttt cggcgcgctt gccggcacca
    10081 cggcggtatt cccacacggt ttcccattcg ggtgcgagcg cggcggagcg gccgcggcgc
    10141 tcgacatgga ccagcagcag agcacacggt gtggcgtcgg cgtcgatcgg gcgtgcgcgc
    10201 aggccggcga aatagtccat cgacagcttc atcgcgtcgg gcagcccggt ggtggcgatg
    10261 cattccctcg cctgtgcgcc gcgctcgccg cgcaccgcga tatcgagcga ttccaccacc
    10321 gtgcggtagc tgcgtccctg gtcgaaccag ggcatcagca acaccaccgc caggctccac
    10381 agcatcacca ggccgatcgc ccagttcgcc ggcgcacgtg cgggcgaccg gggcaggcgg
    10441 gcgacgagga ggatccacac cacgctgatg gcgccgccgg caagcaggcg caggttggcg
    10501 tccggcggca cgaagccggg ggcgttgcgc gcgacgtggc gtgcgagacc gctcggccag
    10561 ccggcgacct gggcgctcca tgccagccag acgaggatgc cgaagacggc caggctcatc
    10621 agcgagaacc agtcgagcgc gctcgcggcg ccgcggcgca ggctggccac gccgccggcg
    10681 gcgagcaggg cgagcgcggg caccagcggg atcagccggg cctgcgagag atcgtcgatc
    10741 gccaccagcc aggccagcac cagtccggcg ctcgccagcg gcagcaggat cgcgagcacg
    10801 ccgggctggc ggcgcgcgcg ccagagcgcc cacaaggcca gcggccacag cggccacatg
    10861 aaccaggcgc cgagctcgag cagtcgcggc agctcggccg ggcgcagcat cgggccgacg
    10921 atccactgcc actcgttgcg cagccacagg ctcaggagtt cggggtgttg caggtggagc
    10981 acgagcggcc acagcgcggc ggtggtcagc gcgaggcaca gtcccagcag cacggcgccg
    11041 ctcgcgcgcg ggctgcggca gtcggcgctc agcagcatcg ccagcggcaa caggggagcg
    11101 ctcatccaca ggccgccgaa gcctgcggag aggaaggcca ggccgctacc catgcccgcc
    11161 ttcaggcccc cggcgagcgg gcgtgtcggc aactccgcga ggccggacag ggtcagtgcg
    11221 agcatggcga agagggcgag caggggctgc gtctcgtgcg catgcaggac caggcctagc
    11281 gtgccgagcg cgagcagcgc ggcgggcgtg cgggcggcgc gaccatgcag ggattcggcc
    11341 gcgcgcgcca gccaccagat cgccagcgcc acgaagaagg cgctcgccag gcgggccccg
    11401 gcgtgcgccg gaagaaaggc cgcggtgagc gcggagaagg ccgaggcgac ccagtaatac
    11461 agcgggggat gctccaccag gggttcgccg gcgagcaggg gaaacagcca ggactcgccg
    11521 cgcagcatcg ccagcacggg cccgaaatgg cgcgcgtcgt cgccgcgcca ggggtcgtgc
    11581 gcggtcaggc cgacgagcag gtacaggccg accagcaggg tcaggccgac cgtgccgtag
    11641 acgcggcgct ggaacagggt gggcgtggcg ggatcgcgga tcatgcgggc attgtgcttc
    11701 ggagtggctg cgcggaccaa gcgcggcgga gtgggcagga cggagagggc gcgggtgcct
    11761 gccggcggcg gggcaataaa aaaggcagct tgcgctgcct tcctcggtgc ctgcggaatc
    11821 ctgccacgac cgcccggggc ggtgcggatc ccggctttgc gacggctcag ctcgcgcgct
    11881 ggacgttgcc gtacttctgg cggaagcgct cgacgcggcc ggcggtgtcg acgatctttt
    11941 gcttgccggt gtagaacgga tggcaggccg agcacacctc gacgtggtac tggggcttgc
    12001 ccagggtcga acgggtcacg aaggtgttgc cgcacgagca ggtgacgttg acgtcggcgt
    12061 acttgggatg gatgtccgct ttcatggtga tttcctgaat cttgcgggcg gaggttgtga
    12121 ccgccctgtg tcgtaaagcg cgagattatc cgccagccgc cccgtcctga caaggaaaaa
    12181 cacgtctgct gtcgtgcgcc gggacggttt ttcgggcggc gaccggtaca atcgcgcgcc
    12241 tgatcaatca ccgacgaggt tccagcgtgc gcctgctgct cgcgcccatg gagggcgtgg
    12301 ccgatttcgt gcttcgcgac gtgctgaccg cgatcggcgg ctacgacctc gcggtgtcgg
    12361 aattcgtccg cgtctccggc accctgctgc cgcggcgcat gttcgagcgc atctgcccgg
    12421 agatcctcgc cggcagccgc accgccgcgg gcacgccggt ggcggtgcaa ctgctcggca
    12481 gcgatcccgt cctgctcgcg gagaacgcgg cgcgcctggc cgcgctgagc ccgcacggca
    12541 tcgacctcaa cttcggctgc ccggccaagg tggtcaatcg ccatggtggc ggtgcgatgc
    12601 tgctcgaccg cccgcgcctg ctgcacgaca tcgccgccgc agtgcgtgcc gccgtgcccg
    12661 cagcgatccc ggtgagcgcc aagatgcgcc tgggcatcgc cgacccctcg cgcgcgctcg
    12721 actgtgcgca ggcgctcgcg gacggtggcg cgcaggccat cgtcgtccat gcgcgcaccc
    12781 gcgagcaggg ctaccgcgcg cccgcgcact gggagtgggt ggcgcgcatc ggcgacgcgg
    12841 tgcgcgtgcc ggtgaccgcc aacggcgagg tgtggagcgt gcgcgactgg gcacgctgcc
    12901 gcgaggtctc cgcagccgag gatgtgatga tcggtcgcgg cgcggtctcc gacccctggc
    12961 tcgcgcgccg catccgcggc gaacgcggca ccgagccgga cgagcgcgac tgggtcgaac
    13021 tgcacccgca cctcctgcgc tactgggagg gcgtgcaggg gcgcgccgcc ccggtgcatg
    13081 cctgtggccg gctcaagctg tggctgggca tgctgcggcg caactacccc gcggcgggcc
    13141 ggctgcacga ggcggtgcgc cgcgtcgtcc acccctcgcg catgaacgag gaactccgcc
    13201 gccacggcat tcccgcgtgc gaccacccgc catctgcccg ggccgacggg ccccggccga
    13261 cgagtcccga ccgatgagag cggcccgatg agctacgcca actcccgcat cccgcagagc
    13321 gcccagcagg gcgtccacga ggcgctcgcc gagcgcgtgc gcaagcacct cgagcatccc
    13381 ttccgcaagc cctgcaccga ctacaaccgt gccgcgctcg ccgaggcgct cgccggctgg
    13441 ggccgcagtg cgccgctgat cctcgatgcc ggctgcggcg tcggccacag caccatccag
    13501 atcgcgcgcc agtttcccga ccactgggtg atcggcgtcg accagtccgc cgaccgcctc
    13561 gcgcgtcgca agccctatcc cgaggcgctg ttgccgaaga acatggtctt cgtgcgcgcc
    13621 gacctggtgg acttctggcg cctgctcgac gaggccgggc tgcgcctcgc gcgccactac
    13681 ctcctctatc ccaacccctg gcccaagatc ggccacctcg gccggcgctg gcacgcgcac
    13741 ccggtgttcc cgtggattcc ccgcctcggt ggcgtgctgg aatgccgcag caactggaag
    13801 gtctacatcg aggagttcgc cttcgcgctc ggcctcgtgc tcgggcgcga ggtcgcctgg
    13861 gaaggcttcg aggccgccac gccgctgacc cccttcgagc gcaagtaccg cgactcgggg
    13921 cagccgttgt accgcatcgg gctggatctc gcggccgcgg gctgattccg caagatcgtg
    13981 cttgcccgcc gcgccgcctt gggccaccct tggcgccggt caaaggagat tgccatgaac
    14041 ggtatggaac aaaggggcgc ggacgcgtcc gcgctgcgca cgatgagcga cgaggatttc
    14101 gaggcgctgg aagagatcct cacctcggac atcgtgcccg aggactgcat ggacctcgag
    14161 atgctcgacg gctacctcgc cggcgtgatg atctcgccgc ggccgatccc ggccgagcgc
    14221 tggatgcccg gcgtgtggtc ggcgcacggc gacgaggtcg gcttcggctc gggcagcggc
    14281 ctgcagcgcg cgatccggct ggtgaaggcg tatcacaacg agctgctcgc cacgctcggc
    14341 ctggacgacg aggacgaggc gtgctgggag ccgttctgct tcgcggtcgc cgagggcgat
    14401 gggctcaagc tcggcgaagc ctggatcgac ggtttcgctc agggcctgga cctgtggccc
    14461 gagggctggg aggaggggct ggacgccgag gcggtcgagg ccgtgcgcga gaccctcgac
    14521 gaggtgctcg cgccctgggg cgaggaggac gccgcgaagg ccgacgacga gacccgcctg
    14581 ggctggctcg ccgacgtggg cgaggcggtc aacgacatct tcgcccactg gcgcgatctg
    14641 ggtctgccgg ccgccgagcc gctgtccacc gaggctccgc gcaagcccgc cagcgccggc
    14701 cccggccgca acgatccgtg cccgtgcggc agcggcaaga aatacaagaa atgctgcggc
    14761 gccaacggcg ccgagcccga gcccgaatcc ggcgaagccg aaacctgagc atcccgggcg
    14821 tgccggcgcg cacaggaccg tcgcgcccgg ggatgcgccg ccggagcggc gcgagtcgcg
    14881 agcgggcgcc ggaacagcgg acgagcgttg gcgagcgtgg acaagccgta gtcgcgactt
    14941 gcgtcgctcc cacaaggaac cgccgcgccc ggggacgcgc tgcgggcgcg atccgatgcg
    15001 agcgggtttc ccgctccgga aaatatttct caaaatgatt tcaggtcact gaaaacaaag
    15061 cgctttgcac cactttccgg cgcctcccgg cggcgctaga atgcgcgccc cacggccctc
    15121 ggccggtcct ctcggcgctc cgttcatgga cgagttcaac ccctgcctcg actgcggcat
    15181 ctgctgcacg cactttcgga tctccttcta ctgggccgag gcggacgacg cgccgggcgg
    15241 tttcgtgccg gcggagatga ccgagaaact caaccacacc ttgcgctgca tgaagggcag
    15301 caaccaggtg ccgcgacgct gcagcgcgct ctccggcgag gtgggccggc aggtcgcgtg
    15361 ttcgatctac gagaaccggc ccaccccctg tcgtgaattt cccgtctatt tcgaggacgg
    15421 tacgcccaac ccgaagtgcg acgagctccg cgccaccatc ggcctgccgc cgctgccgca
    15481 gagtccgctg ccgcgcgcgg cctgatcgcc ggatacgtct cgccagccta tccgccgcgc
    15541 ggccggccca ccgcggcgag cacctcgagc gggccatgtc ggcgcaagcc gtcgatctgg
    15601 gcgcggtcgc gcacgaacag cgagccggcc caggcgagcg aattcagcga cacgtcgtcc
    15661 catttctcgc gtgtgcgggg caccagcagc agcgcgccac ggctgaccag caggttgtag
    15721 ggcggcatcg gatcggcgtc gcagggaagc tccagccgct cgcaggccag gcggtaggcg
    15781 gccagaagga ccgatccggc gtcgtccggg tgcgaccagg cggattcgtc cacccccacc
    15841 gccgcgtgcg cccagggcag ggcgaggttc tccgctgccg ccccgggatg cagcggaggc
    15901 aggaagtctt ccagcgccga ggcctgcgcg ggcacccact gcaggtgctt gtgcgcctgg
    15961 ctcgcccccg cgatgcggcc gccgttgtag aagcccagcc cgccgtgcgc gcccactacc
    16021 gcggcgagcg cggcgaagtc cgcgccgccg agcggcgtgc tctggtcttc gaaacggcgg
    16081 gtcacgatga gcaggtgacg atcgatcacc gggaacttgt tcagcaccac gaggtgatcc
    16141 gcgccgaggg ccgccaccgt gagcgcaggc tcgggcggca ggaaggggtt gaagtcgggc
    16201 cgtcgggcgg tgaccgtgtc gacccgcgcc cgatccttga gcgcgaggga cgatacccaa
    16261 cgcaccgaga agcgcaggcc gtcctgctcc agccagtcga gctcggtacg gatcggctgc
    16321 agtgcgccgc tggcgagcgc cgcggcgatc acgtggtcgg tgcgctcgag gaggtcgtcc
    16381 gcgcgcgagc gggcgctcga cggcggatgg gcggtgagcg ctgcggcgtc ggacggagaa
    16441 cggtgggcgg gcgccgaggc gctggccggg gcggcgtcgc ttgccggatc gcgggcggtg
    16501 ggcgtgctgg tcatgggcgg gcgtgcggga gacaatgccg ccattttgac acgaggcccg
    16561 cgcgcgcagc cgtggcgggc aggagggatc gcgatggcga tacggttcga agtgcgcggt
    16621 gaatacattc agctcgacca gttgctcaag gccgccggtc tggtcgagtc gggcggtgcg
    16681 gcgcatgccg cggtggaaaa cggcctggtg aaggtcgacg gcaagcccga gtcgcgcaag
    16741 cgcgccaagc tgcggccggg acagcgggtg tcgttcgccg gcgaggcggt ggaactcgtg
    16801 gaggaaggtg cgggctagc
//
LOCUS       DAOQBS010000059        16660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2550_length_16660_cov_15.996447, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000059 DAOQBS010000000
VERSION     DAOQBS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 16660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16660
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2550_length_16660_cov_15.996447"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /gene="cyoE"
                     /locus_tag="PKN25_08355"
     CDS             <1..815
                     /gene="cyoE"
                     /locus_tag="PKN25_08355"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNV90949.1"
                     /translation="VALLVLTAWVGMMLATTGLPDFARMLVASLGIAMVSGAAAAFNH
                     VLDHKIDARMARTRLRPLPSGRLSGAQALGFACALAAAGFVLLLVEVNALTAWLTLAG
                     LFGYAVVYTVFLKRATPQNIVIGGVAGALPPVLGWTAMTGTVGAEAWLLMMIVFTWTP
                     PHFWALAIHRVEDYRRAGLPMLPVTHGIEFTRTLVLLYTVLLLAVCLLPYLVGMSGPF
                     YLAGSLALNLRFLHHAWRLKFAPRADTAIRTFRFSITHLMLLFLVLLADHYL"
     gene            complement(823..2721)
                     /gene="recQ"
                     /locus_tag="PKN25_08360"
     CDS             complement(823..2721)
                     /gene="recQ"
                     /locus_tag="PKN25_08360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNV90950.1"
                     /translation="MRAMPTDFPRLPGEPRTPPTAFDQRDVPLGDAAHRVLEHVFGYP
                     AFRGEQGEIVEHVAGGGDALVLMPTGGGKSLCYQIPALLRHGTAIVVSPLIALMQDQV
                     SALVEAGVRAAFLNSSLDMERARAVERALWDGELELLYVAPERLMTPRFLDQLDHLRD
                     TGRLSLFAIDEAHCVSQWGHDFRPEYLQLSILPERYPAIPRIALTATADRQTREEIAE
                     RLNLQAARRFVSSFDRPNIRYTIVEKNDPRRQLLDFIREECPGQAGIVYCLSRRKVEE
                     TAAWLQEQGLAALAYHAGMTQEIRAEHQSRFLREDGLIMVATIAFGMGIDKPDVRFVA
                     HLDLPRSIEGYYQETGRAGRDSLPAQAWMAWGAQDVVQQRRMIDESEANEEFKRLARN
                     RLDVLVGLVEATDCRRQHLLAYFGEQSTPCGNCDNCLHPPQTWDATEAARKALSCVYR
                     TGQRYGAGHLIDVLRGELTEKVVERRHQDITTFGIGSELDEKRWRTVFRQLVARELVA
                     VDHERYNALRLTDAARPLLRGEAEFHLRLEPERSRSRARRRSGASLDIPDGIPTTLFD
                     RLRAWRFATAKERNVPAYVVFQDATLREIAIARPHTLAELAGISGVGDRKLEHYGAAI
                     LQLVAEAG"
     gene            complement(2797..3270)
                     /locus_tag="PKN25_08365"
     CDS             complement(2797..3270)
                     /locus_tag="PKN25_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNV90951.1"
                     /translation="MKGLSKGSLFFCALLSAAAGPAFADALIDATKPERLHEIARGFG
                     SAELDKDSQGDPRITGRIEGTKYGIYFYGCVKGADCDDIQFSASWSGPKVSLEKINEW
                     NRTKRFGKAFLDKDGDPNLEMEVNLDYGVSTRNIEDSFNWWTKALKEYKKVVLEE"
     gene            complement(3474..4388)
                     /locus_tag="PKN25_08370"
     CDS             complement(3474..4388)
                     /locus_tag="PKN25_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNV90952.1"
                     /translation="MNAPNPSETAFSIAAVERDTGLAKDTLRVWERRYGFPAPTRDAN
                     GERVYPSDQVEKLRMIRRLLDHGRRPARIVGASVEELSSMLDSCLCPEGGTPAGRDDG
                     LLQLVRLHRSVELAAALRQALMKQGLQRFVAETVAPLAAAVGEAWMRGEVDVPEEHLF
                     TEQVQNLLRSAIGAHAASEGRPRVLLTTFPEEEHVLGLLMAEAMLVPEGASCLSLGTR
                     TPLADIRSAAVGGGFDIVGLSFSIAYPARQAIDGLLELRALLPEEIGIWAGGGAVRDK
                     QKRLPGIRVVGDLADTLAALQEWRKASA"
     gene            complement(4533..6317)
                     /locus_tag="PKN25_08375"
     CDS             complement(4533..6317)
                     /locus_tag="PKN25_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNV90953.1"
                     /translation="MPSGPKRDGPRREQCPPRPRDPALRCRDAPFPHERPALDTSPLA
                     IYPIVAGLLGGIGLFLLGMHMLTEGLKLAAGRALEGLLERGTATPVRGLGAGMTMTAL
                     VQSSTAVTVASIGFVNTGLLSLQNAMWVIFGSNVGTTLNAWLVAALGFSFRIDAFALP
                     FVGIGATLMLAGRTVRQRALGQALAGFGVLFLGIDALKDTFSGFGATMNLQDHIAPGF
                     TGWLILVGIGTVLTVLMQASGAVIAIIITAAQGGLMSIEAACAMVIGTNIGTTSTAIL
                     SALGATSNARRVAATHVIFNLVTGAVAIALLPLLIGLLGALRNWFEQPATPAVMLAMF
                     HTAFNVLGVLLMVPLARPLRRFLASHFRSREEEIARPRYLDAPSLAVPDLALRALRLE
                     LGRTQSFALTALTAATRVPPDEPWIERQASTLDALAPAIGDYVRKLNAMRLPPALVEA
                     VAHSLRALQYQESAVTAVRQACALATTLGAPPGSELEPIAAAFRQATGALAASADAAR
                     EDFSAPAVEVRLEEAELRYQAFKEALLLEGAHARIDIRTLQDWLRLASLERRAIEQVA
                     KAARMLAVLDGDLTPQQAEEKAGENGVE"
     gene            6409..6675
                     /locus_tag="PKN25_08380"
     CDS             6409..6675
                     /locus_tag="PKN25_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90954.1"
                     /translation="MNAKMLSRRAGMLSAATVLVSFGISAASAAPPQVVAAAAAVAPA
                     GPVVDNTVATPAPASIQTPLELLLFVHRADLPLRPLPTKQKDHS"
     gene            6672..7478
                     /gene="folE2"
                     /locus_tag="PKN25_08385"
     CDS             6672..7478
                     /gene="folE2"
                     /locus_tag="PKN25_08385"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNV90955.1"
                     /translation="MNAPEKFFLPDVQSSADHRALAIQQVGVRGLRYPMAVRDLDGGV
                     QHTVATLEMTVGLPAEVKGTHMSRFVELLEAERDALDLDGLRGLFADMLVRLEAESGR
                     IEARFPWFMKKAAPVSGVESLLDIDARVVVEKTAGGEIRSTLEAVVPVTSLCPCSKKI
                     SAYGAHNQRSHITLSATLRGDLGLRELVRMAEEEASCEVFGLLKRPDEKWVTERAYDN
                     PKFVEDLVRDIALRLRADARVASWRVQSENFESIHNHSAYALVEGENAPE"
     gene            7484..8857
                     /locus_tag="PKN25_08390"
     CDS             7484..8857
                     /locus_tag="PKN25_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV90956.1"
                     /translation="MKMGEIERLHATGEAGRGAEGRRIAVIGGGIAGLSTAWLLAPQH
                     SVTLFEAGSYVGGHTNTIDVTVDGLTHPVDTGFLVFNRRTYPNLCALFALLQVEAVET
                     EMSFGVSLAEPELEWAGTDLASVFAQRGNLVRPAFLAMLRDILRFNRETTRMASEGGM
                     PALSLGEYLDLEDYSQAFRDWYLLPMAAAIWSCPTRTMLAYPLATFVQFCHNHGLLQI
                     LDRPTWMTVKGGGRSYVRRMLARLDDVRVNAPVQRVVRGEDGVWVHTAAGPERFDELV
                     FACHSDQALAILGSEATPDERRILGAVRYQANVAYLHTDTALLPRRPKVWSAWNYLAG
                     KGAPDARPVSVSYLINRLQPLPFQTPVVVSLNPFIAPAADKTFRRIEYAHPVFDHGAI
                     DAQAALPSIQGRERCWFAGAWTGYGFHEDGLKSAVAVVEAMGVEVPWRRGRVPAAKRA
                     GTALEAA"
     gene            8857..9627
                     /locus_tag="PKN25_08395"
     CDS             8857..9627
                     /locus_tag="PKN25_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNV90957.1"
                     /translation="MPDALQPAVCFGAVMHERHVQAHNRFVYPSAFLRLPLGRLDQVR
                     APLLGIERANLFSFRSRDHGARDGSPLLPWIRALLNERGLAAACDGEIVLQTMPRILG
                     FVFNPVSFWFCHDRAGGLRAVLAEVNNTFGERHNYLLHHADLRPIGAGDELRASKRFH
                     VSPFFPVRGEYRFRFEQRGAVHAVSIELWDGGERQLSTRLAGRAEAVGGASMAKWLAR
                     QPFMTLGVVARIHWQALRLALRRVSFFRKPAPPLEETT"
     gene            9627..10868
                     /locus_tag="PKN25_08400"
     CDS             9627..10868
                     /locus_tag="PKN25_08400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNV90958.1"
                     /translation="MNALDHALQPLAGLRLARLPRATRLCLEMLDRLDGGAIAVELPD
                     GVRVRAGHGALVAHLRVRDHAVFDEALARGDIGFGEAWMDGLWDTEDLPGLLRLLSAN
                     RTRLQGAIYGRMFRLLSHRLQHLLRANTRSGSRRNIEAHYDLGNDFYALWLDPTMTYS
                     AAVFASPDESLQDAQLRKYRRILGELGARPGQTILEVGCGWGGFAEVAATEFGCKVLG
                     ITLSPAQLAFARARAERGGFADRVDLELCDYRDVRGQYDHVVSIEMIEAVGERFWPTY
                     FAQLSALLKPGGRCVVQAITIADALFARYRRGTDFIQRHVFPGGMLPSPGAVAQQAGR
                     AGLEVVGDFAFGRDYARTLAHWHRSFDAQAAAVRAQGFPERFLRMWRFYLAYCEAGFD
                     TGDLDVHHYVFAHAAAGSQGG"
     gene            10955..11572
                     /locus_tag="PKN25_08405"
     CDS             10955..11572
                     /locus_tag="PKN25_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNV90959.1"
                     /translation="MSAPIRFPARSPRSLRRSFERFARLPRALGAVLACCMLLALETV
                     HALGLPPQLAASTAGLREVGRGELRWLGFKLYDAALWARAPGEQATADEHVLSIRYAR
                     AVTSARLVELSLDEMRRLGLADEPTLARWGEALAAALPSVAAGDTLTGLHRPGQGAAF
                     WHDGRATAELDDPALAQAFFAIWLDERTREPALRARLLGLEEVTR"
     gene            11569..13113
                     /locus_tag="PKN25_08410"
     CDS             11569..13113
                     /locus_tag="PKN25_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV90960.1"
                     /translation="MSDRARAAPDRHHGGGACARAACAPGAGRAPDADRVEWGALLAY
                     GALGLPLAFAALPIYVHVPRLYAEGLGLSLALVGAVLLAARVVDAITDPLIGWASDRL
                     PRRRLWVALALPALGAGMLGLLMPPAGAGAGWLFALLVAVSLAYSVASIAYNAWGAEV
                     ASTPAARTRFVASREAFALVGVVLAAALPGLLGEGVIGGTAGVGQSAVATEGGAAGGV
                     GASGGGGDGGAAGLARLAWLFLPLLVVFGLWTLWRAPAPPRLAATHAPVWRGLRAALA
                     DAAFLRLLAVFAVNGIAAAIPSATVLFFVADVLQAEALAGAFLALYFLAAAAGLPLWT
                     RLSQRIGKLRAWLAGMALAVAVFAWAGLLGSGDLFAYACICALSGLALGADLTLPPSM
                     LADLLARGRDGRAPARSRALEAGACFGWWSFVTKANLALAAGLALPLLALLGYAPGAR
                     EPTAVAALGAVYGFAPVALKLAAIALLWHGRAVLDPGTGDDPPGIATCVAPTSVDRRR
                     SGNGSG"
     gene            13275..13808
                     /locus_tag="PKN25_08415"
     CDS             13275..13808
                     /locus_tag="PKN25_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HNV90961.1"
                     /translation="MKRFFLMCVALMGLWGCSSVKIDEYRAETPVLELRDYFDGTIDA
                     WGMFQDRSGKVVKRFHVLIEASWQGDTGTLDEQFTYSDGTTQRRVWTITRLGPGRYSG
                     RADDVVGEAAGEAAGNALRWRYVLALPVDGKVYNVDFDDWMFLMDEQVMLNRSLMSKW
                     GFRLGEVTLSFHKRPRP"
     gene            13805..14707
                     /locus_tag="PKN25_08420"
     CDS             13805..14707
                     /locus_tag="PKN25_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV90962.1"
                     /translation="MTAPAARRGGFAPLNTPIREWAGRRVWIVGASSGIGEALALALA
                     RRGARLALSARGRERLEAVCAACDAAAGAGPTAGDAGALANAAVEPAHRAESPAPAGV
                     LALALDLSRAGEIERARDRLLAAWGGIDLVVFNAGTYRPLRAWELTAEAVRETLETNL
                     VGTMSGTAAVLPTLLAQGHGAIALVGSVAGYGGLPKALVYGPSKAALINFAEVLHLDL
                     SPRGIGVFLIDPGFVATPLTAQNDFAMPALQTPEQAAAAIVDGFARGDFEIHFPKRFT
                     RALKLLQLLPRPLYFALIRRATGV"
     gene            14719..15198
                     /locus_tag="PKN25_08425"
     CDS             14719..15198
                     /locus_tag="PKN25_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNV90963.1"
                     /translation="MTDPKPPEHLPSSAASAEPVRQSPARRLARFYETLTPAALPGLD
                     QLYAPDARFKDPFNEVAGTAAIRRIFAHMFATTEAPRFEVTDCIEQGEQAMLGWAFHF
                     ALRGRTLTVRGVTHLRFDADGRVVLHRDYWDAAEELYEKLPLIGAPLRWLRRRMSAE"
     gene            15502..16107
                     /locus_tag="PKN25_08430"
     CDS             15502..16107
                     /locus_tag="PKN25_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNV90964.1"
                     /translation="MPSSLCAPLRTLLLAVIAPLLLLAGCTPAEPVFKSTDITGTSYG
                     KTLRLTDHNGRERTLADFKGKVVTIFFGYTQCPDVCPTALSGMSTVMQELGPDAERVQ
                     VLFVTVDPERDTPELLAQYVPVFDARFLGLYGTPEKIAELAKEFRVFYRKSGDLAGHY
                     TIDHTAGTYVFGPDGRPRLYVRHAEDPQVVVADIKALLAGK"
     gene            complement(16171..>16660)
                     /locus_tag="PKN25_08435"
     CDS             complement(16171..>16660)
                     /locus_tag="PKN25_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV90965.1"
                     /translation="LVKIATTKAQRKLFFNLRGMKQDSSTLQPAEVRSIAAQLGVKPE
                     EVVEMETRLSGRDIPLDAGSDDEDERFAPIAYLPDPHAEPSEQVEQAQLARLQDSGLR
                     DALASLDERSRAIVQRRWLAEGDSATLHELAAEYGVSAERIRQIEAKAMQKMRGMLAA
                     VA"
BASE COUNT         2187 a         6008 c         5892 g         2573 t
ORIGIN      
        1 tggtggcgct gctggtgctg accgcctggg tgggcatgat gctcgccacc accgggctgc
       61 ccgacttcgc gcgcatgctg gtggcgagcc tgggcatcgc catggtgtca ggcgcggcgg
      121 cggccttcaa tcacgtgctc gaccacaaga tcgacgcccg catggcgcgt acccggctgc
      181 gtccgctgcc cagcgggcgc ctgagcggcg cgcaggcgct gggcttcgcg tgcgcgctcg
      241 ccgcggcggg cttcgtgctg ctgctggtgg aggtcaatgc gctcaccgcg tggctgacgc
      301 tggccgggct gttcggctac gcggtcgtct acaccgtgtt cctcaagcgc gccacccccc
      361 agaacatcgt catcggcggg gtcgcgggcg cgctgccgcc ggtgctggga tggacggcga
      421 tgacgggcac ggtcggcgcc gaggcctggc tgctgatgat gatcgtcttc acgtggaccc
      481 cgccacactt ctgggcgctg gccatccacc gcgtcgagga ctaccgccgc gccggcctgc
      541 cgatgctgcc ggtcacccat ggcatcgagt tcacccgcac gctggtgctg ctctacaccg
      601 tgctgctgct tgcggtgtgc ctgctgccgt atctggtggg gatgagcggg ccgttctacc
      661 tggcgggcag cctggccctg aacctgcggt tcctgcacca cgcctggcgg ctcaagttcg
      721 ctccgcgcgc cgacacggcg atccgcacct tccgcttctc gatcacccac ctgatgctgc
      781 tgttcctcgt gctgctggcg gaccactatc tgtgatcgag gatcagcccg cttcggcgac
      841 cagctgcagg atcgccgccc cgtagtgctc cagcttgcga tcgcccacgc cgctgatgcc
      901 ggccagctcc gccagcgtgt gcggacgggc gatggcgatc tcgcgcagcg tcgcgtcctg
      961 gaagaccacg taggccggca cgttgcgctc cttggcagtg gcgaagcgcc aggcgcgcag
     1021 gcggtcgaag agcgtggtgg ggatgccgtc ggggatatcc aggcttgcgc cgctgcgccg
     1081 ccgggcgcgg ctgcggctgc gctcgggctc caggcgcagg tggaactcgg cctcgccgcg
     1141 cagcagcggg cgggccgcgt cggtgaggcg cagcgcgttg tagcgctcgt ggtccaccgc
     1201 gaccaactcg cgcgcgacga gctggcggaa caccgtgcgc cagcgcttct cgtcgagctc
     1261 gctgccgatg ccgaaggtgg tgatgtcctg gtggcggcgc tcgaccacct tttcggtgag
     1321 ctcgccgcgc agcacgtcga tcaggtggcc ggcgccgtag cgctggccgg tgcggtagac
     1381 gcaggagagg gccttgcgcg ccgcctcggt ggcatcccag gtctgcggcg gatgcaggca
     1441 gttgtcgcag ttgccgcagg gggtcgactg ttcaccgaaa tacgccagca ggtgctggcg
     1501 gcggcagtcg gtggcctcga ccaggccgac cagcacgtcg agccggttgc gcgcgaggcg
     1561 cttgaactcc tcgttcgcct cggactcgtc gatcatgcgg cgctgctgca ccacgtcctg
     1621 cgcgccccag gccatccagg cctgcgccgg caggctgtcg cgccccgccc ggccggtctc
     1681 ctggtagtag ccctcgatcg agcgcggcag gtcgaggtgg gcgacgaagc gcacgtcggg
     1741 cttgtcgatg cccatgccga aggcgatcgt cgccaccatg atcagcccgt cctcgcgcag
     1801 gaagcggctc tggtgctcgg cgcggatctc ctgcgtcatg ccggcgtggt aggccagggc
     1861 ggcgaggccc tgctcctgca gccaggcggc ggtctcctcg accttgcgcc gcgacaggca
     1921 atacacgatg ccggcctggc cgggacattc ctcgcggatg aagtcgagca gctggcggcg
     1981 cgggtcgttc ttctcgacga tggtgtagcg gatgttgggg cggtcgaagc tggagacgaa
     2041 gcggcgcgcc gcctgcaggt tgaggcgctc ggcgatctcc tcgcgggtct ggcggtcggc
     2101 ggtggcggtg agcgcgatgc gcgggatggc cgggtagcgc tcgggcagga tggagagctg
     2161 caggtactcg gggcggaagt cgtggcccca ctgcgacacg cagtgcgcct cgtcgatcgc
     2221 gaacagcgag agccggccgg tgtcgcgcag gtggtcgagc tggtcgagga agcgcggtgt
     2281 catcaggcgc tcgggggcga cgtagagcag ctcgagctcg ccgtcccaga gcgcgcgctc
     2341 caccgcgcgt gcgcgctcca tgtccaggct ggagttgagg aaggcggcgc gcacgccggc
     2401 ctcgaccagc gcgctcacct ggtcctgcat cagcgcgatc aacggcgaca ccacgatcgc
     2461 ggtgccgtgg cgcagcagcg ccgggatctg gtagcacagc gacttgccgc caccggtcgg
     2521 catcagcacc agcgcgtcgc cgccgccagc cacgtgctcg acgatctccc cctgctcgcc
     2581 gcggaaggcg gggtagccga agacgtgctc gagcacgcga tgcgcggcgt cgccgagggg
     2641 aacgtcgcgc tggtcgaacg cagtcggggg cgtgcgtggt tcgccgggca ggcgggggaa
     2701 atcggtgggc atggcgcgca ttttaccgcg ccgggtctcg tccgatgaca cggaccggtg
     2761 gcagccgggc gtctgcgacg cccgggcccg catccgtcat tcctcgagga cgaccttctt
     2821 gtattccttg agggccttgg tccaccaatt gaaggagtcc tcgatgttcc gggtgctgac
     2881 gccgtagtcg aggttcacct ccatctcgag gttcggatca ccgtccttgt cgagaaaagc
     2941 cttgccgaag cgcttggtcc ggttccattc gttgatcttt tccagcgaga ccttggggcc
     3001 gctccagctg gcggagaact ggatgtcgtc gcagtcggcg cccttcacgc agccgtagaa
     3061 ataaatgccg tatttcgtgc cctcgatccg tccggtgatg cgcggatccc cctggctgtc
     3121 cttgtcgagc tcggcggagc cgaatcctcg ggcgatctcg tggaggcgtt caggctttgt
     3181 cgcgtcgatc agcgcgtcgg cgaacgcggg accggctgct gcgctcagca gggcgcaaaa
     3241 aaacagactt cccttcgaaa gtcccttcat ggaatcactc ctgttgtgga aacacgggcg
     3301 gtggcgcatc cggcaaaagc gcgcgccctg acggacgcgc gcgagcgcaa gaatatgctt
     3361 gcgagattct acggggcggc ttggcgcgga tgccgtgaag ttttaccgca catgaacccg
     3421 gaataggccc gtcgtcagcg ggcgcgcgca cggtcgcagg gcgcgtgcgg cgctcaggcg
     3481 ctcgccttgc gccattcctg cagtgccgcc agcgtgtccg ccaggtcgcc taccacacgg
     3541 atgcccggca ggcgcttctg cttgtcgcgg accgcgccgc cgccggccca gatcccgatt
     3601 tcctccggca gcagcgcgcg cagctcgagc aagccgtcga tcgcctggcg tgccggatag
     3661 gcgatgctga aggacagccc gacgatgtcg aaacctccac ccaccgccgc gctgcggatg
     3721 tccgcgagcg gcgtgcgcgt gccgagcgac aggcagctcg cgccctccgg caccagcatc
     3781 gcctccgcca tcagcaggcc gagcacgtgc tcctcctcgg ggaaggtcgt cagcagcacg
     3841 cgcggtcggc cttcgctcgc ggcatgcgcg ccgatcgcgc tgcgcagcag gttctgcacc
     3901 tgctcggtga acagatgttc ctcgggaaca tccacctcgc cgcgcatcca cgcctcgccc
     3961 accgccgccg ccagcggcgc caccgtctcg gcgacgaagc gctgcagccc ctgcttcatc
     4021 agcgcctggc gcagtgcagc ggcgagctcc acgctgcgat gcaggcgcac gagctgcagc
     4081 agaccgtcgt cgcgaccggc aggcgtgccg ccctccgggc acaggcagga atcgagcatc
     4141 gaagacagct cctcgacgct cgcgcccacg atccgggccg gtcgacggcc gtggtcgagc
     4201 aggcggcgga tcatgcgcag cttttccacc tggtcggacg ggtagacacg ctcgccgttg
     4261 gcgtcgcgcg tcggcgcagg gaagccgtaa cgccgctccc acaccctcag ggtgtccttc
     4321 gccaggcccg tgtcgcgttc gacggcagcg atgctgaagg cggtttcaga cgggttcggg
     4381 gcgttcatcg gcagcagctt tcgggaggtg gtcgttaaga cgatggtagg cgggagaatt
     4441 tgttttgtcc aggacaaatg aggcgccgac gctggaggcg actccatttc ccgaccgcga
     4501 tgcatgcgcc cgcgccccca gccgatcggc gctcactcca ccccgttttc ccccgccttc
     4561 tcctccgcct gctgcggcgt caggtcgccg tccagcaccg cgagcatgcg cgccgccttg
     4621 gccacctgct cgatcgcgcg gcgctccagg ctggccaggc gcagccagtc ctgcagcgtg
     4681 cgaatgtcga tgcgggcgtg cgcaccctcc agcagcagcg cctccttgaa ggcctggtag
     4741 cgcagctccg cctcctccag ccgcacctcg accgccggcg cggagaagtc ctcgcgcgcc
     4801 gcgtccgcgc tcgcggccag cgccccggtg gcctggcgga aggccgcggc gatcggctcg
     4861 agctcgctgc ccggcggcgc gccgagcgtg gtggccagcg cgcacgcctg gcgcaccgcg
     4921 gtgaccgcgc tctcctgata ctgcagcgcg cgcaggctgt gcgccaccgc ctcgaccagc
     4981 gccggcggca ggcgcatggc gttgagcttg cgcacgtagt cgccgatcgc cggcgccagc
     5041 gcatcgagcg tgctcgcctg acgctcgatc cagggctcgt ccggcggcac ccgcgtggcg
     5101 gcggtgagcg cggtgagggc gaaggactgc gtgcggccca actccaggcg cagcgcgcgc
     5161 agcgccaggt cgggcaccgc cagcgagggc gcgtcgagat agcgcgggcg ggcgatctcc
     5221 tcctcgcggc tgcggaagtg gctcgccagg aatcggcgca gcgggcgtgc cagcggcacc
     5281 atcagcagca ccccgagcac gttgaaggcg gtgtggaaca tcgccagcat caccgccggc
     5341 gtggccggct gctcgaacca gttgcgcagc gcgccgagca ggccgatcag cagcggcagc
     5401 agcgcgatcg ccaccgcgcc ggtcaccagg ttgaagatca cgtgggtggc cgcgacccgg
     5461 cgcgcgttcg aggtcgcgcc cagcgccgac aggatcgccg tcgaggtcgt gccgatgttg
     5521 gtgccgatca ccatcgcgca cgccgcctcg atcgacatca gcccgccctg ggcggcggtg
     5581 atgatgatcg cgatcaccgc gcccgaggcc tgcatcagca ccgtcagcac ggtgccgatg
     5641 ccgaccagga tcagccagcc ggtgaagccg ggtgcgatgt ggtcctgcag gttcatggtc
     5701 gcgccgaaac ccgagaaggt gtccttgagc gcgtcgatgc cgaggaagag cacgccgaag
     5761 ccggccagcg cctggccgag cgcgcgctgg cgcaccgtgc gtccggccag catcagggtc
     5821 gcgccgatgc cgacgaaggg cagcgcgaag gcgtcgatcc ggaagctgaa gccgagcgcc
     5881 gccaccagcc aggcgttgag cgtggtgccg acgttggagc cgaagatcac ccacatcgcg
     5941 ttctgcagcg acagcagccc ggtgttgacg aagccgatgc tcgccaccgt gaccgcggtg
     6001 gaggactgca ccagcgcggt catggtcatg ccggcgccga ggccgcgcac cggcgtggcg
     6061 gtgccgcgct cgagcaggcc ctcgagcgcg cggccggcgg cgagcttgag gccctcggtg
     6121 agcatgtgca tgccgagcag gaacaggccg atgccgccga gcaggccggc gacgatcgga
     6181 tagatggcga gcgggctggt gtccagggcg ggcctctcgt gcgggaaggg ggcgtcacga
     6241 cagcgtaatg ccggatcgcg cgggcgggga gggcattgtt cgcggcgggg accatcgcgc
     6301 tttggaccgg atggcaaaca atttcaattt gtcctggaca aattcttgac agccctgcgg
     6361 tgctcgccta tcgtggaatc gtcatccacg cttgtccagg acacggccat gaacgcgaaa
     6421 atgctctcca ggcgcgccgg catgctctcc gccgctacgg tgctcgtctc cttcgggatc
     6481 tccgcggctt cggccgcacc gccccaggtc gtcgcggcgg cagccgccgt cgctcctgcc
     6541 gggcccgtcg tggacaacac ggtcgcgacc ccggccccgg cctcgatcca gaccccgctc
     6601 gaactcctgc tcttcgtgca ccgcgcggat cttccgctgc gccccctccc caccaagcaa
     6661 aaggatcatt catgaacgcc ccggaaaaat tcttcctgcc cgacgtgcaa tccagcgccg
     6721 accaccgcgc gctggcgatc cagcaggtcg gcgtgcgcgg tctgcgctac cccatggcgg
     6781 tgcgcgatct cgacggcggc gtgcagcaca ccgtcgccac gctggagatg acggtcggcc
     6841 tgcccgccga ggtgaagggc acccacatgt cgcgcttcgt cgagctgctc gaggccgagc
     6901 gcgacgcgct cgatctcgac ggtctgcgcg gcctgttcgc cgacatgctg gtgcggctcg
     6961 aagccgagag cggccgcatc gaggcgcgct ttccctggtt catgaagaag gccgcgccgg
     7021 tttcgggcgt cgaaagcctg ctcgacatcg acgcccgcgt ggtggtggaa aagaccgcgg
     7081 gcggcgagat ccgtagcacc ctcgaggcgg tggtgccggt caccagcctg tgcccgtgct
     7141 ccaagaagat ctccgcctac ggcgcgcaca accagcgctc gcacatcacc ctgtcggcca
     7201 cgctgcgcgg cgacctcggc ctgcgcgagc tggtgcgcat ggccgaggaa gaagcctcgt
     7261 gcgaagtctt cggcctgctc aagcgcccgg acgagaagtg ggtcaccgag cgcgcctacg
     7321 acaaccccaa gttcgtcgag gacctcgtgc gcgacatcgc gttgcgcctg cgtgccgatg
     7381 cgcgcgtggc ctcgtggcgg gtgcagtcgg agaacttcga gtccatccac aaccattccg
     7441 cctacgccct cgtcgagggc gagaacgcgc cggagtaatc gccatgaaga tgggtgagat
     7501 cgagcgcctg cacgccacgg gcgaggcggg ccggggtgcc gaggggcggc ggatcgccgt
     7561 gatcggcggc ggcatcgccg gtctgtcgac cgcctggctg ctcgcgccgc agcacagcgt
     7621 gacgctgttc gaagcgggca gctacgtcgg cggtcacacc aacaccatcg acgtgaccgt
     7681 cgacggcctc acccatccgg tggacaccgg cttcctggtc ttcaaccgcc gcacctaccc
     7741 caacctgtgt gcgctgttcg cgctgctgca ggtcgaggcg gtggagaccg agatgagctt
     7801 cggcgtcagc ctggccgaac ccgagctcga atgggccggc accgaccttg ccagcgtgtt
     7861 cgcccagcgc ggcaacctgg tgcgtcctgc cttcctcgcc atgttgcgcg acatcctgcg
     7921 cttcaaccgc gagaccacgc ggatggcgag cgaaggtggc atgccggccc tgtcgctcgg
     7981 cgagtatctc gacctggagg actactcgca ggccttccgc gactggtacc tgctgcccat
     8041 ggccgcggcg atctggtcgt gtccgacgcg caccatgctc gcctatccgc tcgcgacctt
     8101 cgtgcagttc tgccacaacc acggactgct gcagatcctc gatcgtccga cctggatgac
     8161 ggtgaagggc ggcggacgca gctacgtgcg ccgcatgctc gcccgcctcg acgacgtgcg
     8221 ggtgaacgcg ccggtgcagc gcgtggtacg cggcgaggac ggggtgtggg tgcacaccgc
     8281 ggccgggccg gagcgcttcg acgagctcgt cttcgcctgt cacagcgacc aggccctggc
     8341 catcctcggc agcgaggcca cgccggacga gcgccgcatc ctcggcgccg tgcgctacca
     8401 ggccaacgtc gcgtacctgc acaccgacac cgcgctcctg ccacgccgcc cgaaggtgtg
     8461 gtcggcgtgg aactacctcg ccggcaaggg cgcgcccgac gcgcgtccgg tgtcggtcag
     8521 ctacctcatc aaccgcctgc agccgctgcc tttccagacc ccggtcgtgg tgtcgctcaa
     8581 ccccttcatc gcgcccgcgg ccgacaagac cttccgtcgc atcgagtacg cccacccggt
     8641 gttcgatcac ggcgcgatcg acgcccaggc cgcgctgcct tccatccagg gccgcgagcg
     8701 ctgctggttc gccggcgcct ggaccggcta cggcttccat gaggacggcc tcaagtcggc
     8761 ggtggcggtg gtcgaggcga tgggcgtgga agtgccgtgg cggcgcggcc gcgtgccggc
     8821 ggcgaagcgg gccgggacgg cgctggaggc ggcgtgatgc ccgacgcgct ccagcccgcg
     8881 gtgtgcttcg gcgcggtcat gcacgagcgc cacgtgcagg cgcacaaccg cttcgtgtac
     8941 ccgagcgcct tcctgcgcct gccgctcggc cgcctggacc aagtgcgcgc tccgctgctc
     9001 ggcatcgagc gtgccaacct gttcagcttc cgcagccgcg accacggcgc gcgcgacggc
     9061 tcgcccttgc tgccgtggat ccgcgccctc ctgaacgagc gcgggctggc cgcggcatgc
     9121 gacggcgaga tcgtgctgca gaccatgccg cgcattctcg gcttcgtctt caacccggtg
     9181 agcttctggt tctgccacga ccgcgcgggc ggcctgcgtg cggtgctggc cgaggtgaac
     9241 aacaccttcg gcgagcgtca caactacctg ctgcaccacg ccgacctgcg ccccatcggc
     9301 gcgggcgatg agctgcgcgc cagcaagcgc ttccatgtct cccccttctt cccggtgcgc
     9361 ggcgagtacc gcttccgctt cgagcagcgc ggcgccgtgc atgcggtgtc gatcgagctc
     9421 tgggacggcg gcgagcgcca gctctccacc cgcctggcgg gccgcgccga ggccgtcggt
     9481 ggggccagca tggcgaagtg gctcgcgcgc cagcccttca tgaccctggg cgtcgtcgcc
     9541 cgcatccact ggcaggcgct gcgcctggcc ctgcggcggg tgagcttctt ccgcaagccc
     9601 gcgccgccgc tcgaggagac cacctgatga acgcactcga ccacgccctg cagccgctcg
     9661 ccggcctgcg cctcgcgcgc ctgccgcgcg ccacccggct gtgcctggag atgctcgacc
     9721 gcctcgacgg cggcgcgatc gcggtggagc tgcccgacgg cgtgcgcgtg cgcgccggcc
     9781 acggtgcgct cgtcgcccac ctgcgcgtgc gcgaccacgc cgtgttcgac gaggcgctcg
     9841 cacgcggcga catcggcttc ggcgaagcct ggatggacgg cctgtgggac accgaggacc
     9901 tccccggcct gctgcgcctg ctctcggcca accgcacccg cctgcagggc gcgatctacg
     9961 gccgcatgtt ccgcctgctc agccaccgcc tgcagcacct gctgcgcgcc aacacgcgca
    10021 gcggctcgcg gcgcaacatc gaggcccact acgacctcgg caacgacttc tatgcgctct
    10081 ggctcgaccc caccatgacc tactcggcgg cggtgttcgc ctcgcccgac gagtcgctgc
    10141 aggacgccca gctgcgcaag taccgccgca tcctcggcga gctcggcgcg cggcccgggc
    10201 agaccatcct ggaggtgggc tgcggctggg gcggcttcgc cgaggtcgcc gccaccgagt
    10261 tcggctgcaa ggtgctgggc atcacgctgt cgccggcgca gctggcgttc gcgcgtgcgc
    10321 gcgccgagcg cggcggcttc gccgaccggg tggatctgga gctgtgcgac taccgcgacg
    10381 tgcgcggcca gtacgaccat gtggtgtcga tcgagatgat cgaggcggtg ggcgagcgct
    10441 tctggccgac ctacttcgcc cagctctccg cactgctgaa gccgggcggg cgctgcgtgg
    10501 tgcaggcgat cacgatcgcc gacgcgctgt tcgcgcgcta ccggcgcggc accgacttca
    10561 tccagcgcca cgtcttcccc ggcggcatgc tgcccagccc gggcgcggtg gcgcagcagg
    10621 ccgggcgcgc cgggctcgag gtggtgggcg acttcgcctt cgggcgcgac tatgcgcgca
    10681 cgctcgcgca ctggcatcga agcttcgacg cgcaggctgc agcggtgcgc gcccagggct
    10741 tccccgagcg cttcctgcgc atgtggcgtt tctatctcgc ctactgcgag gcgggcttcg
    10801 acaccggcga cctcgacgtg catcactacg tgttcgcgca tgccgccgca gggtcgcaag
    10861 gcggctgaat ggtgatgggc gcggcggagg gaagcgatct gtgccgaccc cctggccgcg
    10921 ccgtccccgc atttcagggc ctggaggtga ccccatgagc gccccgatcc gctttcccgc
    10981 acgctccccg cggagcctgc gtcgcagctt cgagcggttc gcccgcctcc cgcgcgcgct
    11041 cggcgcagtg ctggcgtgct gcatgctgct ggcgctggag acggtgcacg cgctcggcct
    11101 gccgccgcag ctcgcggcga gcacggccgg gctgcgcgag gtcggccgcg gcgagctgcg
    11161 ctggctcggc ttcaagctct acgacgccgc gctgtgggcg cgcgcgccgg gcgaacaggc
    11221 cacggcagac gagcacgtgc tgtcgatccg ctacgcgcgc gcggtcacgt ccgcgcgtct
    11281 ggtcgagctc agcctcgacg agatgcgccg cctcggtctc gccgacgagc cgacgctggc
    11341 gcgctggggc gaggcgctcg ccgccgcgct gccttcggtc gcggccggag acacgctgac
    11401 cggcctgcac cgtccggggc agggcgcggc cttctggcac gacggccgcg ccaccgccga
    11461 actcgacgac ccggcgctgg cgcaggcctt cttcgccatc tggctcgacg agcgcacccg
    11521 cgagcccgcg ctgcgcgcac gcctgctcgg gctcgaggag gtcacgagat gagcgaccgc
    11581 gcacgcgccg cgccggatcg ccaccacggg ggcggagcat gtgcccgcgc ggcctgcgcg
    11641 cctggcgccg ggcgtgctcc ggatgccgac cgcgtcgagt ggggtgcgct gctcgcctac
    11701 ggcgccctcg gcctgccgct cgccttcgcg gcgctgccga tctacgtgca tgtgccgcgc
    11761 ctgtatgcgg aaggcctggg cctgtcgctc gcgctggtcg gtgccgtgct gctcgcggcg
    11821 cgcgtcgtcg acgccatcac cgacccgctc atcggctggg ccagcgaccg cctgccgcgg
    11881 cgccggctgt gggtcgcgct ggcgctgccc gcgctcgggg ccggcatgct cggtctgctc
    11941 atgccgcccg cgggggccgg ggcgggctgg ctgttcgcgc tgctggtcgc ggtgtcgctg
    12001 gcctactcgg tggcgagcat cgcctacaac gcctggggcg ccgaggtcgc ctccaccccc
    12061 gcggcgcgca cgcgcttcgt cgccagccgc gaggccttcg cgctcgtcgg tgtggtgctg
    12121 gcggcggcgc tgccggggct gctgggcgag ggggtgatcg gtggcacggc tggcgtcggc
    12181 cagtcggcgg tcgccactga aggcggcgcc gcgggcggtg tcggtgcgtc agggggcggt
    12241 ggcgacggcg gcgcggcggg gctcgcacgg ctcgcctggc ttttccttcc gctgctcgtg
    12301 gtcttcggcc tgtggacgct gtggcgtgcg cccgctccgc cgcggctcgc tgcgacgcac
    12361 gcgccggtgt ggcggggcct gcgcgcggcg ctcgccgacg cggccttcct ccgcctgctc
    12421 gcggtgttcg cggtcaatgg catcgccgcc gcgatcccct cggcgacggt gctcttcttc
    12481 gtcgccgacg tgctgcaggc cgaggcgctt gccggcgcct tccttgccct gtatttcctc
    12541 gccgccgcgg ccgggctgcc gctgtggacg cggctgtcgc agcgcatcgg caagctgcgc
    12601 gcctggctcg ccggcatggc gctcgccgtg gcggtgttcg cctgggcagg cctgctcggc
    12661 agcggcgatc tgttcgccta tgcctgcatc tgcgcgctct cgggcctggc gctcggcgcc
    12721 gacctcaccc tgccgccttc catgctcgcg gacctgctcg cgcgcgggcg ggacgggcgt
    12781 gcgccggccc gctcgcgcgc gctcgaggcg ggtgcctgct tcggctggtg gagcttcgtc
    12841 accaaggcca acctggcgct cgccgccggc ctggcgctgc ccctgctcgc gctgctcggc
    12901 tacgccccgg gcgcgcgcga gcccactgcg gtcgccgcgc tcggggcggt gtacggcttc
    12961 gcaccggtcg ccctcaagct cgccgccatc gccctgctct ggcacgggcg agccgtgctc
    13021 gatcccggca cgggagacga cccgcccggg atcgcgactt gcgtcgctcc cacaagcgtc
    13081 gaccggcgcc gcagtggaaa cggcagcggg tgaggcggcc cgtaggagcg acgcaagtcg
    13141 cgataacgcg gcgaccggcg ctgggggggg aacggcagcg ggtgaggcgg cccgtaggag
    13201 cgacgcaagt cgcgatcgca gcaccgacgc tgcgacgacg cagcaagccg gaatctggtc
    13261 ggaggagagg aatcgtgaaa cgctttttcc tgatgtgcgt cgccctgatg gggctttggg
    13321 ggtgcagcag cgtgaagatc gacgaatacc gtgccgaaac gcccgtgctc gagctgcgcg
    13381 actacttcga cggcacgatc gacgcctggg gcatgttcca ggaccgctcg ggcaaggtgg
    13441 tcaagcgctt ccacgtgctc atcgaggcga gctggcaggg tgacaccggc acgctcgacg
    13501 aacagttcac ctactccgac ggcacgaccc agcgccgcgt gtggacgatc acgcgcctcg
    13561 gtcccgggcg ctacagcggc cgcgccgacg acgtggtcgg cgaggccgcc ggcgaggcgg
    13621 ccggcaacgc gctgcgctgg cgctacgtgc tggcgctgcc ggtggatggc aaggtgtaca
    13681 acgtcgactt cgacgactgg atgttcctga tggacgagca ggtcatgctc aaccgctcgc
    13741 tgatgagcaa gtggggcttc cgcctcggcg aggtcacgct gtccttccac aagaggccgc
    13801 gcccgtgacc gcaccggctg cccgccgcgg cgggttcgcg ccgctcaaca cgccgatccg
    13861 cgaatgggcg gggcggcggg tgtggatcgt cggtgcgtcc tcgggcatcg gcgaggcgct
    13921 cgcgctcgcg ctcgcccgca ggggcgcgcg cctggcgctc tccgcgcgcg ggcgcgagcg
    13981 cctcgaggcg gtgtgcgcgg cctgcgacgc ggctgccggc gcgggcccga ccgccgggga
    14041 cgcgggcgcg ctcgcgaatg cggccgtcga gcctgcgcac cgcgccgagt cgcccgctcc
    14101 cgctggagtg ctcgcgcttg cgctcgactt gtcgcgcgcg ggcgagatcg aacgggcgcg
    14161 cgaccggctg ctcgccgcct ggggcggcat cgatctcgtg gtcttcaacg ccggcaccta
    14221 ccgcccgttg cgcgcctggg agctcactgc ggaggccgtg cgcgagacgc tggagaccaa
    14281 cctcgtcggc accatgagcg gcaccgcggc agtcctgccg accctgctcg cgcagggtca
    14341 cggcgccatc gcgctggtcg gcagcgtggc cggctacggc ggactgccga aggcgctcgt
    14401 ctacggcccg tccaaggcgg cgctgatcaa cttcgccgag gtcctccacc tcgacctgtc
    14461 gccgcgcggc atcggcgtct tcctgatcga ccccggcttc gtcgccacgc cgctgaccgc
    14521 gcagaacgac ttcgccatgc ccgcgctgca gacgcccgag caggcggccg ctgcgatcgt
    14581 cgacggcttc gcgcgcggcg acttcgagat ccacttcccg aagcgcttca cgcgcgcgct
    14641 caagctcctg cagctgctgc cgcggccgct gtatttcgcg ctgatccgcc gcgcaaccgg
    14701 tgtgtagggc ttgccgccat gaccgacccg aagcctccgg agcaccttcc atcgagcgcc
    14761 gcttcggcgg aacccgtacg gcaatctccc gcccggcgcc tggcgcgctt ctacgagacg
    14821 ctcacccccg ccgcactgcc tgggctcgac cagctctacg cccccgacgc gcgcttcaag
    14881 gaccccttca acgaggtcgc cggcaccgcc gccatccgcc gcatcttcgc gcacatgttc
    14941 gccaccaccg aggcgccgcg cttcgaggtc acagactgca tcgagcaggg cgagcaggcg
    15001 atgctgggct gggcgttcca cttcgcgctg cgcggccgca cgctgacggt gcgcggtgtc
    15061 acgcatctgc gcttcgacgc cgacggccgc gtggtgctgc accgcgacta ctgggatgcc
    15121 gccgaggagc tgtacgagaa gctgccgctg atcggcgcgc cgctgcgctg gctgcggcgc
    15181 aggatgtcgg cggagtgagg tgtgggggga ggtgacggga gcactgcgat cgcgacttgc
    15241 gtcgctccca caggggcctt cggaaggcgc ggcggttttt cgtaggagcg acgcaagtcg
    15301 cgatccccgc cgcgcgggga atccgcgtcg gcgcgtgttc cagcgctgcg atcgcgactt
    15361 gcgtcgcttc cacaggggcc ttcggacacg cggcggtttc tcgtgggagc gacgcaagtc
    15421 gcgattcccc gttgcggcgc gggttccggc gtgctgacga agggactatc atcgactcct
    15481 gatccgcttg cccgtcccct catgccctcc agcctctgcg cgccgctgcg caccctcctc
    15541 ctcgccgtca tcgcgcccct tctcctgctc gccggctgca cgcccgccga gccggtgttc
    15601 aagagcaccg acatcaccgg cacgagctat ggcaagacgc tgcgccttac cgaccacaac
    15661 ggccgcgagc gcacgctcgc cgacttcaag ggcaaggtgg tgacgatctt cttcggctac
    15721 acgcaatgcc ccgacgtctg ccccaccgcg ctgtcgggca tgagtacggt gatgcaggag
    15781 ctcggccccg acgccgagcg cgtgcaggtg ctcttcgtca ccgtcgaccc cgagcgcgac
    15841 acgcccgagc tgctcgcgca gtacgtgccg gtgttcgatg cgcgcttcct cggcctctac
    15901 ggcacgcccg agaagatcgc cgagctggcg aaggaattcc gcgtgttcta ccgcaagagc
    15961 ggtgacctcg ccggccacta caccatcgac cacaccgccg gcacctacgt cttcggcccc
    16021 gacggccggc cgcggctgta cgtgaggcac gccgaggacc cgcaggtggt cgtggcggac
    16081 atcaaggcct tgctggcggg gaagtgaggg cgcgcgctcc aagggaaaac ggcggcctcg
    16141 ggccgccgtt tcgttgtctg gggcgggtgg tcacgccacc gcggccagca tcccgcgcat
    16201 cttctgcatc gccttggcct cgatctggcg gatgcgttcg gccgacacgc cgtattccgc
    16261 cgccagctcg tgcagcgtgg cgctgtcgcc ctcggcgagc cagcgccgct gcacgatcgc
    16321 gcggctgcgc tcgtccaggc tggcgagcgc gtcgcgcagg ccgctgtcct gcaggcgggc
    16381 gagctgagcc tgctcgacct gctcggaggg ctcggcgtgc ggatcgggca ggtaggcgat
    16441 gggtgcgaag cgctcgtcct cgtcatcgct gccggcgtcg agcgggatgt cgcgaccgct
    16501 cagccgcgtc tccatctcga ccacttcctc gggcttcacg cccagctgcg ccgcgatcga
    16561 gcgcacctcg gcgggctgca gcgtgctgct gtcctgcttc atgccgcgca ggttgaagaa
    16621 cagcttgcgc tgcgccttgg tggtggcgat cttcaccagg
//
LOCUS       DAOQBS010000060        16609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2566_length_16609_cov_14.369578, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000060 DAOQBS010000000
VERSION     DAOQBS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16609
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2566_length_16609_cov_14.369578"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKN25_08440"
     CDS             <1..470
                     /locus_tag="PKN25_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNV90966.1"
                     /translation="TSGGVSVGEEDHVKPAVEAEGSLDMWKIAMKPGKPLAYGRVHGA
                     AFIGLPGNPVSSFVTFLLMVRPFLLATQGVSEVAPAAMQLRADFDWPRPDRRREFLRA
                     RMNAQGGVELFANQGSAALESTVWANGLVDIPAGTVIACGETVRFLPYGDLLH"
     gene            525..785
                     /gene="moaD"
                     /locus_tag="PKN25_08445"
     CDS             525..785
                     /gene="moaD"
                     /locus_tag="PKN25_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HNV90967.1"
                     /translation="MTSKVKILYFASLREAVGCAGEELDLPAGVGTVGALRSHLAARG
                     EGWQALAAGRNVRAALNQKMAGADASLDAGDEVAFFPPVTGG"
     gene            788..1327
                     /gene="moaE"
                     /locus_tag="PKN25_08450"
     CDS             788..1327
                     /gene="moaE"
                     /locus_tag="PKN25_08450"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HNV90968.1"
                     /translation="MVEARAQDAAEVSVQHEDFDVGGEIARLGEGRRDVGAVASFVGL
                     VRDANDGSGVRAMTLEHYPGMTERALEEIVEQAKARWSLLSVRVIHRYGRLEPADRIV
                     FVGVTSAHRGEAFAACEFIMDYLKTRAPFWKCEETPQGARWVDARESDDSAAARWAAS
                     GAQVPAGEGEDWDDIELRR"
     gene            complement(1455..1961)
                     /locus_tag="PKN25_08455"
     CDS             complement(1455..1961)
                     /locus_tag="PKN25_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNV90969.1"
                     /translation="MASKQEQLNELQKKNLEAAMRLAQMSIENSQRIMEIQVTTAKRL
                     FEEGVQNAKALSAAKDPQGAMEMRASYAQRTTEEMLACARQIAEITAATQAEFGRLVG
                     EQLSSGSTDMFEAMQKMFTGMPIADQNALGAMQTAMDTTRAAFEQMTRASTQAFQAFT
                     QQGGKGRR"
     gene            complement(2180..2716)
                     /locus_tag="PKN25_08460"
     CDS             complement(2180..2716)
                     /locus_tag="PKN25_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNV90970.1"
                     /translation="METLRRLLRHLWLDADDARRALGEAALDRLEARVRSSETGHTGQ
                     ICLCVEASLPPSYLWRHLARREALASVIRDRAITLFGKQRVWDTEDNNGVLVYLLLAE
                     HRIEIVADRALARQLEPAAWQALVDRLATPCREARFEDALAAAVDCLDQLLRAAFRGA
                     GATGLASGGGLSDRPVLR"
     gene            complement(2716..3612)
                     /locus_tag="PKN25_08465"
     CDS             complement(2716..3612)
                     /locus_tag="PKN25_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNV90971.1"
                     /translation="MAQQLWRALLTLAALFATGLALAQPLQPVPALQARVTDLTATLS
                     AAQVRALEGKLAAFEAAHGSQIAVLLVRSTAPEDIAAYAFRVADAWKIGRREVGDGVL
                     LLVAKDDRKVRIEVARALEGAIPDLAARRIIDGAITPALRRGDFAGGIDAGVGGLSAL
                     IRGENLPAPEPAARGGGGAGIEDLGALLFVGVPILGAVLVGMLGRKPGAFATGAVTGL
                     IVHLLTASLFLALVGGVLAVVFVLALGIGGGGRGGPPHRGGGPVIRGGSLGGLGGGRL
                     GGGGFRSGGGGSFGGGGASGSW"
     gene            complement(3616..4254)
                     /locus_tag="PKN25_08470"
     CDS             complement(3616..4254)
                     /locus_tag="PKN25_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNV90972.1"
                     /translation="MDSRPARRPFRHLGRLALPLVLAASLTLSGCGYNDFQRLDEQTR
                     AAWSEVLNQYQRRADLIPNLVETVKGEAAFEQETLTRVIEARARATSIQVTPGMLDDP
                     QALERFQQAQGQLGSALSRLLAVAENYPDLKANQGFQDLRVQLEGTENRVTVARNRYI
                     DAVQQYNVLARSFPSNLTAMVFGYQPKPGFTVADEAAISAPPRVDFGGQGGR"
     gene            4415..4741
                     /locus_tag="PKN25_08475"
     CDS             4415..4741
                     /locus_tag="PKN25_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNV90973.1"
                     /translation="MSDNAVHPFDARGVAKRFRHAAIFGALESLEDGEVMRFVNDHDP
                     LPLLNQIQQRYGNQVGIHYVAREPGNIVIDFTIQLGAPAAVGAPAAAVAGGGGCGGGG
                     GHGCCS"
     gene            complement(4738..4947)
                     /locus_tag="PKN25_08480"
     CDS             complement(4738..4947)
                     /locus_tag="PKN25_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90974.1"
                     /translation="MPMTYEQHLDEVATLLYERYDQSEKAAIKLVMAAQEDGFFSGHD
                     DDPSICTLERAQADARAVFRKYGKA"
     gene            complement(4959..6299)
                     /gene="gorA"
                     /locus_tag="PKN25_08485"
     CDS             complement(4959..6299)
                     /gene="gorA"
                     /locus_tag="PKN25_08485"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HNV90975.1"
                     /translation="MKEYDLIAIGGGSGGVATARRAAEYGARVLLIEAARLGGTCVNV
                     GCVPKKVMWYASGIAQALRDAPGYGFAEVAGRFDWATLKQRRDAYIERLNGIYAGMLD
                     KSGVELKRGFARFSGPRVVEVEGERFTAPHIVISTGGRPALPDLPGAELGIDSDGFFA
                     LEAQPRRVAVVGAGYIAVELAGVFHGLGSEVSMLVRGDRLLRPFDAMLRDELAAQMQE
                     DGIALRFGTRARALRRQADGSLLVDCGEAGSLEVDALVWATGRQPNTDRLQLAAAGIE
                     ADAKGTIPTDAFQNTNVPGIYAIGDITGRAELTPVAIAAGRRLALRLFRGGSDSKLDY
                     ENIPTVVFSHPAIGTVGLTEEEARKRFADVKVYSTRFTAMYNALTEHRPKTSMKLVCV
                     GADERIVGAHVIGDGADEMLQGFAVAVKMGARKADFDDTVAIHPTSAEEFVTMR"
     gene            complement(6364..6921)
                     /locus_tag="PKN25_08490"
     CDS             complement(6364..6921)
                     /locus_tag="PKN25_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNV90976.1"
                     /translation="MSFARLARALAPAVLLATLAPNAGAADDATTRLYDHSLRRLHAD
                     ESVRLADRYAGQPVLVVNTASHCGFTRQFRELEAVHQRYRERGLKVAGFPSDDFRQEA
                     ADEAETAEVCFQNFGVTFDMYAPIHVTGTEAHPLFREIARQSAAPEWNFHKYVIDRNG
                     RVVAAFGSRTKPDAPEVIAAIEKAL"
     gene            complement(7043..7681)
                     /locus_tag="PKN25_08495"
     CDS             complement(7043..7681)
                     /locus_tag="PKN25_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90977.1"
                     /translation="MNIGKLRHKATAGLIVAALLAGPAFADKPDGAGGGKPDRVQGGK
                     PDHGGPQERGDKRPDKRSEQGNRDVRGDETRGRGGDRDGGQARGREQDRDREHRTDRA
                     GSSPRVSAYFSDRHREVVRQYYGDRFRAGDCPPGLAKKGNGCMPPGQAKKWRIGAPLP
                     RDVVWYDAPGDIVLRLGMPPEGHRFVRVAADILLISVGTGMVVDAIEDLSRL"
     gene            complement(7865..9082)
                     /gene="ppk2"
                     /locus_tag="PKN25_08500"
     CDS             complement(7865..9082)
                     /gene="ppk2"
                     /locus_tag="PKN25_08500"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNV90978.1"
                     /translation="MNKTEETVPVVDTASTRSKKPRTAPRAKDAGAARAKSKAAGPAR
                     PIAAGDTAPDFSSTGIEAFEIEQAIDAAQDSKLVAVKDILARAAADPQAKREDALEAI
                     LDGASPDDIHMLRRALMRADLPQAPGAHPDDELSPDWRKGGYPYRNLMSRKAYEKQKY
                     RLQVELLKLQAWVKETGQRVVILFEGRDAAGKGGTIKRFMEHLNPRGARVVALEKPSE
                     LERGQWYFQRYIQHLPTAGEIVLFDRSWYNRAGVERVMGFCTPDEYNEFMRQVPEFER
                     NLVRSGIHLIKFWFSVSREEQRRRFKERESHPLKQWKLSPIDLASLDKWDEYTKAKEA
                     MFFYTDTADAPWTVVKSDCKKRARLNALRYVLHKLPYANKDVSLIGPLDPLLVGRANV
                     VYERGEKDAVALL"
     gene            9223..10176
                     /locus_tag="PKN25_08505"
     CDS             9223..10176
                     /locus_tag="PKN25_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV90979.1"
                     /translation="MPLTKETRSLLDMAYRVGAPRFHELAVAQARHSAQKLHFAFRPE
                     APAVASTAEVPIPRPDTSALLARLYRPFGSHVDDRLPLVIFAHGGGWCIGDVASYDVA
                     CRELANGSGCAVLSVEYRLAPEHPFPAAPEDMRLAFDWSVDNADLLGIDPARIALAGD
                     SAGGNLAIVTALALRAAEVKPAFLLLIYPSTEIRSARPSRERYAEGFFLDRESLQWFF
                     ERYLPGVDTEDWRVSPMRAASLAGLPPMQVICAECDPLVDDCLAFVERVRSEGGEVAL
                     RVFEGVVHGFYTLGKLIPEAREAVALSAAALRSHLAECAQS"
     gene            complement(10224..11087)
                     /locus_tag="PKN25_08510"
     CDS             complement(10224..11087)
                     /locus_tag="PKN25_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV90980.1"
                     /translation="MSSFDTQFEAFAEQVQQELREGVTSFPTVFDLSVRVQKLADAPE
                     TSLAQIAGAVQTEPLLAAKVIRMANTLTLNPYRGQITSLSDAIQRIGLSTLRCLAFAV
                     AAEQLARDHRSRQMRLIASGLWMHSIDVAAWSCAFAHHLKRPDPDAAMLAGLMLDIGQ
                     FYLLARAARYPALEGDMGRFAEVVTTWDEPVRDAVLEALEMPERIITACAADPFANTE
                     WPPAELGDVVFLATLAAESPNPFDTLLGSRQRGELLEASVADGLDPDGFDTLMSEARA
                     SRQELISAACG"
     gene            complement(11172..11774)
                     /gene="xpt"
                     /locus_tag="PKN25_08515"
     CDS             complement(11172..11774)
                     /gene="xpt"
                     /locus_tag="PKN25_08515"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNV90981.1"
                     /translation="MLPDPDLIRPYGPLVRRIEAEATVIGDQILKIDHFLNHRIEPSF
                     ITEMGQELAKRLARFEPSVVLTAEASGIAPGLVVAQALDVPLVYAKKYAPEVESPAIA
                     RVIPSPTKGGETKLVLSQRYIVPTDRVVIVDDFLSNGRTAVALVEMAREAGAEVLCAS
                     FIVEKRFKHGHAAIEALGVPVCTLAQVEALENGHVRLTQP"
     assembly_gap    11843..11942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12019..12483
                     /gene="tadA"
                     /locus_tag="PKN25_08520"
     CDS             12019..12483
                     /gene="tadA"
                     /locus_tag="PKN25_08520"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNV90982.1"
                     /translation="MNDEDYMRAALEQARAAGAADEVPVGAVVVCGGEIVGRGFNQPI
                     GRHDPTAHAEIMALRDAAAHLGNYRLPGCELYVTLEPCAMCSGAIMHARIARVVFGAR
                     DPKTGVAGSVLDLFAEPRLNHHATIEGGLLADECGRMLSSFFAARRGRTLVA"
     gene            12484..12963
                     /locus_tag="PKN25_08525"
     CDS             12484..12963
                     /locus_tag="PKN25_08525"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNV90983.1"
                     /translation="MHLRVDLGRQCLELFGADGACIRRYAVSTGERGAGERSGSLCTP
                     RGRHRIRARIGAGAPAGAVFRGRRPTGEVWTAEFAAAHPGRDWILSRILWLCGEEPGR
                     NRGGEVDTMRRYIYLHGTGDDQPMGVARSHGCVRMRNHDIIELFELVPAGTVVEIVE"
     gene            12960..13385
                     /locus_tag="PKN25_08530"
     CDS             12960..13385
                     /locus_tag="PKN25_08530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV90984.1"
                     /translation="MKGVRIEVVGWDEAEARVMPVRTGVFVVEQGVPEEIERDAFDAV
                     CRHAIAHDAEGRVIATGRLLPDGHIGRMAVLREARGAGVGGAVLQALIAEAARRGLNE
                     VALNAQTHALDFYLRHGFEPVGEVFMEAGIPHRAMRRPV"
     gene            complement(13419..14219)
                     /locus_tag="PKN25_08535"
     CDS             complement(13419..14219)
                     /locus_tag="PKN25_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNV90985.1"
                     /translation="MCLIVFAWQAHPDYPLVVAANRDEYLARPAAPAHWWVDAPELLA
                     GRDLEAGGTWMGLTRGGRFAALTNYRDPSRHIAGAPSRGALVREALEDVRPAPATLQA
                     VQASAGNYAAFNLLVGDADRLGVLESTTGGLRMLEPGYYGLSNHLLDSPWPKLVKTRA
                     ALAGKLEALPTSGPAAATEDALVAGLLGLLRDPTPAPDPHLPATGVSLEWERWLSPAF
                     IRAPGYGTRCSSVVLRDRKGRTRLVEWTWDADGELRSRVEHAFGAGTT"
     gene            complement(14274..15311)
                     /locus_tag="PKN25_08540"
     CDS             complement(14274..15311)
                     /locus_tag="PKN25_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HNV90986.1"
                     /translation="MTAWTEHLARMGATLDETARSIAFASPADEARATETATIAVPLL
                     HLGTIRSQGPDSAAFLHNLVSNDVKHMEADTAAWNSFNSPKGRMIASFLVWTEEGGHA
                     LALSADILPAFLKKFSMYVLRSKVKLADASAEVALIGLAGPQAVAIAQAAGAALPAED
                     MRQAVSAAGVRCIRLGAQRLVLAVATDAAPALFDALVAAGALRAGTAAWQLGMIRAGL
                     ALVTAPTQEEFVAQMLNYELIGGVDFHKGCYPGQEIVARTQYLGKLKKRTYRLALPAG
                     STAAPGTDVYAPDFGEQSAGKLVNVAPTADGGVEALAVIQSSSAEAGEIRVGAPDGPR
                     ADLLDLPYALA"
     gene            15453..16466
                     /gene="mltG"
                     /locus_tag="PKN25_08545"
     CDS             15453..16466
                     /gene="mltG"
                     /locus_tag="PKN25_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNV90987.1"
                     /translation="MKRFLPRLLLLLAVLAILAAMIAATGWWYAHRPLALAAERVDFT
                     VARGMGMRQAAAAIERAGVGVDARLLALLARLTKRDARIKAGSYEVHAGITPWQLILK
                     LSDGDVTQGELLLVEGWTFRQVRQALESHPDLEADTAGLGEAEILARIGASAQNAEGL
                     FFPDTYLFDKRSGALAVLRRAHEAMQARLDKAWAERDPATPLASPYEALILASIVEKE
                     TGRPEDRALVASVFANRLRIGMRLQTDPTVIYGLGPEFDGRLRRAHLDADHPWNTYTR
                     AGLPPTPIAMPGEAALRAALKPEKSDFLYFVARGDGSSEFSRDLAAHNRAVDKYIRNG
                     GGG"
BASE COUNT         2248 a         5600 c         6105 g         2556 t
ORIGIN      
        1 tcaccagcgg cggcgtgtcg gtgggcgagg aagaccacgt caagccggcg gtcgaggccg
       61 agggcagcct ggacatgtgg aagatcgcga tgaagccggg caagccgctg gcctacgggc
      121 gggtgcatgg tgccgccttc atcggtctgc cgggcaaccc ggtgtcgagc ttcgtgacct
      181 tcctgctgat ggtgcggccc ttcctgctcg ccacccaggg tgtgagcgag gtcgctccgg
      241 cggcgatgca gttgcgcgcc gacttcgact ggcccaggcc cgaccgccgg cgcgagtttc
      301 tgcgcgcgcg catgaacgcg cagggcgggg tggaactctt cgccaaccag ggctcggcgg
      361 cgctcgagtc caccgtgtgg gccaacggcc tggtcgacat cccggccggc acggtgatcg
      421 cgtgcggcga gacggtgcgc ttcctgccct acggcgacct gctgcactga ggcgcggatg
      481 gggacgagcg gacgcggacg ggcaatcaag ggaacgagac ggacatgacg agcaaggtga
      541 agattctgta tttcgcgagc ctgcgcgagg ctgtggggtg cgccggcgag gagctggacc
      601 tgcccgccgg cgtcggcacg gtgggggcct tgcgcagcca cctggccgcg cgcggcgagg
      661 gctggcaggc gctggcggcc gggcgcaacg tgcgcgcggc gctcaaccag aagatggcgg
      721 gcgccgatgc gtcgctcgat gccggcgacg aggtcgcctt ctttccgccg gtgaccggag
      781 gctgacgatg gtggaagcga gagcgcagga cgccgccgag gtcagcgtcc agcacgagga
      841 cttcgacgtc gggggcgaga tcgcgcgcct cggcgaggga cggcgcgacg tcggcgcggt
      901 ggcgagcttc gtcggcctgg tgcgcgacgc caacgacggc agcggcgtgc gcgcgatgac
      961 gctggagcac tacccgggca tgaccgagcg cgcgctcgag gagatcgtcg agcaggcgaa
     1021 ggcgcgctgg tcgctgctct ccgtgcgcgt catccaccgc tacggccgcc tggagcccgc
     1081 cgaccgcatc gtcttcgtcg gcgtgacgag cgcgcatcgt ggcgaggcct tcgccgcctg
     1141 cgagttcatc atggactacc tgaagacgcg ggccccgttc tggaagtgcg aggagacgcc
     1201 gcagggcgcg cgttgggtcg atgcgcgcga gagtgacgac tcggccgctg cgcgctgggc
     1261 cgcctccggt gcgcaggtcc ccgcgggcga gggcgaggac tgggacgaca tcgagctgcg
     1321 gcgctgaggg ggcgaggacg gccggggagg ccgccctttg cgggttcttg cgggatgcgg
     1381 atcaaataaa acggcgccgc aaggcgccgt tttcaatcgc agcgggaacg gcccgccaca
     1441 cgaggcggag gcgttcagcg acgacccttg ccaccctgct gggtgaaggc ctggaaggcc
     1501 tgggtggagg cgcgcgtcat ctgttcgaag gccgcacggg tggtgtccat cgcggtctgc
     1561 atcgcgccca gcgcgttctg atcggcgatc ggcatgccgg tgaacatctt ctgcatggct
     1621 tcgaacatgt cggtgctgcc cgagctcagc tgctccccca ccagccggcc gaactcggcc
     1681 tgggtggcag cggtgatctc ggcaatctgg cgtgcgcacg cgagcatctc ttcggtggtg
     1741 cgctgcgcgt agctcgcgcg catttccatc gcgccctgcg ggtccttcgc ggccgagagg
     1801 gccttggcgt tctgcacgcc ttcctcgaac agacgcttgg ccgtggtgac ctggatctcc
     1861 atgatgcgct gcgagttctc gatcgacatc tgggccaggc gcatcgccgc ctcgaggttc
     1921 ttcttctgaa gttcgttcaa ctgttcctgt ttgctggcca taggctcctc tccactcgca
     1981 cgtatcggtt gtctgacttt ttccgggctt ggcgccctcg ggacggtgtc ccttgttgtt
     2041 ctcgggccgg cacatcctca ccgacttcac catcccggct tccacaatag cacagctcgc
     2101 ttgcggctcc gcggtgcatc cggtgcttcg aaggcgtgac gggtgcgtat gatgctgcgc
     2161 tgcaacagct tcggccgctt cagcgcagca ccgggcggtc ggagagaccg ccaccgctcg
     2221 caaggccggt ggcgcccgcc ccgcggaaag ccgcgcgcag cagctggtcc aggcaatcca
     2281 ccgcggccgc gagcgcgtcc tcgaagcgcg cttcgcggca gggcgtggcg aggcgatcga
     2341 ccagtgcctg ccaggcggcg ggttcgagct ggcgtgcgag cgcacgatcc gcgacgatct
     2401 cgatgcggtg ctcggcgagc agcagataga ccagcacgcc gttgttgtcc tcggtgtccc
     2461 acacgcgctg cttgccgaac agcgtgatcg cgcggtcgcg gattacgctc gcgagcgcct
     2521 cgcggcgcgc gaggtggcgc cagaggtagc tcggcggcag gctggcctcg acgcacaggc
     2581 agatctgccc ggtgtggccg gtctcgcttg agcgaacacg cgcttccagg cggtcgagcg
     2641 ccgcctcgcc gagtgcgcga cgggcgtcgt cggcgtcgag ccagaggtgg cgcaggagcc
     2701 tgcgcagtgt ctccatcacc agctccccga ggcgccgccg ccaccgaaac tgcctccgcc
     2761 gccggaacgg aacccgccac ccccgagcct gccgccgccc aggccgccga ggctgccgcc
     2821 gcggatgacc ggccctccgc cgcgatgcgg cggaccgccg cgaccgccgc cgccgatgcc
     2881 gagcgcgagc acgaagacca cggcgagcac gccgccgacg agggcgagaa agaggctggc
     2941 ggtaagcaga tgcacgatga gcccggtgac ggcgcccgtc gcgaaggccc cgggtttgcg
     3001 cccgagcatg cccaccagca ccgcgccgag aatcggcacg ccgacgaaaa gcagggcgcc
     3061 gaggtcctcg atgccggcgc ctccgccgcc gcgtgcggcg ggttccggcg caggcaggtt
     3121 ctcgccgcgg atcagcgcgg acaggcctcc cacaccggcg tcgatgccgc cggcgaagtc
     3181 gccacggcgg agggccggcg tgatcgcgcc gtcgatgatg cgccgggcgg cgaggtcggg
     3241 gatcgcgccc tcgagcgcac gggcgacctc gatgcgcacc ttgcggtcgt ccttcgccac
     3301 cagcagcagc acgccgtcgc ccacctcgcg acggccgatc ttccaggcat ccgcgacacg
     3361 gaaggcgtag gcggcgatat cctcgggcgc ggtgctgcgc acgagcagca cggcgatctg
     3421 gctgccatgc gcggcctcga aggcggcgag cttgccctcg agcgcgcgca cctgcgccgc
     3481 cgacagcgtt gcggtgaggt cggtcacacg cgcctgcagt gccggcaccg gctgcagcgg
     3541 ctgggcaagc gccagcccgg tcgcgaacag tgcggcgagc gtcagcagcg cgcgccagag
     3601 ctgctgcgcc atcgctcagc gtccgccctg gccaccgaag tcgacccggg gcggcgccga
     3661 gatcgcggcc tcgtcggcca ccgtgaagcc gggtttgggc tggtagccga acaccatcgc
     3721 agtgaggttg gacgggaagc tgcgcgccag cacgttgtat tgctggacgg cgtcgatgta
     3781 gcggttgcgc gccacggtga cgcggttctc ggtgccctcg agctgtacgc gcaggtcctg
     3841 gaagccctgg ttcgccttga ggtcggggta gttctcggcg accgcgagca gacgcgacag
     3901 cgcgctgccg agctggccct gtgcctgctg gaagcgctcc agcgcctgcg ggtcgtcgag
     3961 cattccgggg gtgacctgga tcgatgtggc gcgggcgcgc gcctcgatca cgcgggtcag
     4021 cgtctcctgc tcgaaggcgg cttcgccctt gaccgtctcg accaggttgg ggatgagatc
     4081 ggcgcggcgc tggtactggt tgagcacctc cgaccacgcg gcgcgggtct gttcgtcgag
     4141 gcgctggaag tcgttgtaac cgcagccgga cagggtcagc gaggcggcga ggacgagcgg
     4201 tagcgcgagg cggccgaggt gacggaaagg ccgacgagcg gggcgggaat ccatttcgct
     4261 tctccggaag atgccggtga aaagatcttc actctagcag tgcgcacggg gtttttgtcc
     4321 ttgctcaagg attccgcctg cgcgcggtac taggattcgc gagctgcgat tccggccgca
     4381 ggttcgagac cggaccccca atggaagatc caagatgtcc gataacgctg ttcacccctt
     4441 cgatgcgcgc ggcgttgcca agcgcttccg ccatgccgcc atcttcggtg cgctcgagtc
     4501 gctcgaggac ggcgaggtca tgcgcttcgt caacgaccac gatccgctgc cgctgctgaa
     4561 ccagatccag caacgctacg gcaaccaggt cggcatccac tacgtcgccc gcgagccggg
     4621 caacatcgtc atcgacttca ccatccagct cggcgctccg gctgcggtgg gtgcgcccgc
     4681 tgccgcggtc gccggtggcg gcggttgcgg cggcggtggc ggccacggct gctgctccta
     4741 agcctttccg tacttgcgga agaccgcgcg ggcatcggcc tgcgcgcgtt ccagggtgca
     4801 tatcgacgga tcgtcgtcgt gcccgctgaa gaacccgtcc tcctgggcgg ccatcaccag
     4861 cttgatggcc gccttttcgc tctggtcgta gcgctcgtat aaaagcgtgg cgacctcgtc
     4921 caggtgttgc tcgtaagtca tcggcatcgc tcgctccctt agcgcatggt gacgaattct
     4981 tcggcgctgg tcgggtggat cgcgaccgtg tcgtcgaagt ccgccttgcg cgcgcccatc
     5041 ttcaccgcca ccgcgaagcc ctgcagcatc tcgtcggcgc cgtcgccgat cacgtgggcg
     5101 ccgacgatgc gctcgtccgc gccgacgcag accagcttca tcgaggtctt cggacggtgc
     5161 tcggtgagcg cgttgtacat ggcggtgaag cgcgtgctgt agaccttcac gtcggcgaag
     5221 cgcttgcgcg cctcttcctc ggtgaggccg acggtgccga tggccgggtg gctgaacacc
     5281 acggtgggga tgttctcgta gtccagcttg ctgtcggacc cgccgcggaa caggcgcagc
     5341 gcaagccggc gacccgccgc gatcgccacc ggggtgagct cggcgcggcc ggtgatgtcg
     5401 ccgatcgcgt agatgccggg cacgttggtg ttctggaacg cgtcggtggg gatcgtaccc
     5461 ttggcgtcgg cctcgatgcc ggccgcggcg agttgcaggc ggtcggtgtt gggctggcgg
     5521 cctgtggccc acaccagcgc gtccacctcg aggctgccgg cctcgccgca gtcgacgagc
     5581 aggctgccgt cggcctggcg ccgcagcgcg cgcgcccggg tgccgaagcg aagcgcgatg
     5641 ccgtcctcct gcatctgcgc ggcgagttcg tcgcgcagca tggcgtcgaa ggggcgcagc
     5701 aggcgatcgc cgcgcacgag catggagacc tcgctgccca ggccgtggaa aacgccggcc
     5761 agctccaccg cgatgtatcc cgcgccgacc acggcgacgc gacgcggctg cgcctcgagg
     5821 gcgaagaagc cgtccgaatc gatgccgagc tcggcgccgg gcaggtcggg cagggcgggg
     5881 cggccgccgg tggagatcac gatgtggggt gcggtgaagc gctcgccttc gacctcgacc
     5941 acccgcgggc cggagaagcg tgcgaagccg cgcttcagct cgacgccgga cttgtccagc
     6001 atgccggcgt agatgccgtt gaggcgctcg atgtaggcat cacgacgctg cttgagcgtg
     6061 gcccagtcga agcgcccggc gacctccgcg aagccgtatc cgggggcatc gcgcagcgcc
     6121 tgcgcgatgc ccgaggcgta ccacatcacc ttcttgggca cgcaaccgac gttgacgcag
     6181 gtgccgccga ggcgggcggc ctcgatcagc agcacgcgcg caccgtactc cgcggcgcgg
     6241 cgggcggtgg cgacgccacc gctgccgccg ccgatggcga tcaggtcgta ttccttcatg
     6301 ggttctccct ttccgtgggc ggctggcggc cgcctcgttg tcgcgcttgg tggggcgcgg
     6361 cgctcagagc gccttctcga ttgccgcgat cacctcgggg gcgtcgggct tggtgcggct
     6421 gccgaaggcc gccaccacgc ggccgttgcg gtcgatcacg tacttgtgga agttccactc
     6481 cggcgccgcc gactggcgtg cgatctcgcg gaacaaggga tgcgcttcgg tgccggtgac
     6541 gtggatcggc gcgtacatgt cgaaggtgac gccgaagttc tggaagcaga cctcggcggt
     6601 ctcggcctcg tcggcagcct cctggcggaa gtcgtcggac ggaaagccgg cgaccttgag
     6661 cccgcgctcc ctgtagcgct ggtgcaccgc ctcgagctcg cggaactgcc gcgtgaagcc
     6721 gcagtggctg gcggtgttga ccaccagcac gggctggccg gcgtagcggt ctgcgagccg
     6781 caccgactcg tcggcgtgca gacggcgcag gctgtggtcg tataggcgcg tcgtcgcatc
     6841 gtcggcagcg ccggcgttgg gggcgagggt ggcgagcaga acggcggggg cgagggcacg
     6901 ggcaagacgg gcgaaggaca tgatcggtct cctggcagga acggcaagcg gtggaccggg
     6961 cgatgcgcga tcggttcgcc ttgtgggcgc ggcacgcgca gcgaggcacc ggacccgcct
     7021 cgtggcggat ccgcgccggg gctcagaggc ggctgagatc ctcgatcgca tcgaccacca
     7081 tcccggtgcc gacactgatc agcaggatgt cggcggcgac gcggacgaag cgatgacctt
     7141 cgggcggcat gccgaggcgt agcacgatgt cgcccggcgc gtcgtaccac accacgtcgc
     7201 gcggcagcgg cgcgccgatg cgccacttct tcgcctgtcc cggcggcatg cagccgttgc
     7261 ccttctttgc cagtccgggt gggcagtcgc cggcacggaa gcggtcgccg taatactgac
     7321 gcaccacctc gcggtgacgg tcggagaagt aggccgaaac gcgcggcgac gagccggcgc
     7381 ggtcggtgcg atgttcgcgg tcgcggtcct gctcgcggcc gcgcgcctgg ccaccgtcgc
     7441 ggtctccacc gcgcccgcgc gtctcgtcgc cacgcacgtc ccgattgccc tgctcgctgc
     7501 gtttgtcggg gcgcttgtcg ccgcgttcct ggggaccgcc gtgatcgggt ttgccgccct
     7561 ggacgcgatc gggtttgccg ccgccggccc cgtccggctt gtcggcgaag gccgggccgg
     7621 cgaggagcgc ggcgacgatc aggccggcgg tggccttgtg gcgaagtttg ccgatgttca
     7681 tcttgttctc ccttgtcgtt tgtgccgtgg ggcgcgcggg ggcggccagc cagcgaggga
     7741 catcccgccg gcgacccgtg cctgcaggat agatcgccac ggacgcggcc cgcacgacgg
     7801 cttacctctt gtcaccagcc gtcgatcccg gatgcaaatg tgcgctgcgt cggaccgcag
     7861 acgcttacag cagcgccacg gcgtccttct cgccgcgctc gtacaccacg ttggcacggc
     7921 ctaccagcaa gggatcgagc gggccgatga gggagacatc cttgttggca tagggcagct
     7981 tgtgcagcac gtagcgcagc gcgttgaggc gtgctcgctt cttgcagtcg gacttgacta
     8041 cggtccatgg cgcgtcggcg gtgtcggtgt agaagaacat cgcctccttg gccttggtgt
     8101 attcgtccca cttgtcgagc gaggcgaggt cgatcggcga cagcttccac tgcttgagcg
     8161 ggtgcgactc gcgctccttg aagcggcgac gctgctcctc gcggctcacc gagaaccaga
     8221 acttgatcag gtggatgccg ctgcgcacca ggttgcgctc gaactcgggc acctggcgca
     8281 tgaattcgtt gtactcgtcg ggcgtgcaga agcccatgac gcgctcgacg ccggcgcggt
     8341 tgtaccagga gcggtcgaac agcacgatct cgccggcggt gggcaggtgc tggatgtagc
     8401 gctggaagta ccattggccg cgctcgagct cggagggctt ctccagcgcc accacgcgcg
     8461 cgccgcgcgg gttgaggtgc tccatgaagc gcttgatcgt gccgcccttg ccggcggcgt
     8521 cgcggccctc gaacaggatc accacgcgct ggccggtctc cttgacccag gcctgcagct
     8581 tgagcaactc gacctgcagg cggtacttct gcttttcgta ggccttgcgc gacatcaggt
     8641 tgcggtaggg gtagccgccc ttgcgccagt cgggcgagag ctcgtcgtcc ggatgggcgc
     8701 ccggcgcctg cggcaggtcg gcgcgcatca gcgcgcggcg cagcatgtgg atgtcgtcgg
     8761 gcgaggcgcc gtcgaggatg gcctcgagcg cgtcctccct tttggcctgc ggatccgcag
     8821 ccgctcgggc caggatgtcc ttgaccgcga cgagcttgga gtcctgcgcg gcgtcgatcg
     8881 cctgctcgat ctcgaaggcc tcgatgcccg tgctgctgaa atcgggcgcg gtgtcgccgg
     8941 ccgcgatcgg acgtgcgggg ccggcggcct tggacttcgc gcgcgcggcg cccgcgtcct
     9001 tggcgcgcgg agccgtgcgc ggcttcttgc tgcgggtgga ggcggtgtcc actacgggga
     9061 cggtttcctc ggtcttgttc atgatgacgc ttctcccttg tctgcacgaa ttctaatccc
     9121 gtgccttccg cagggcacgc ccgtcgtggg gtgcgacggg tagaatcgcg gactccgccc
     9181 gcggccgggc gtggcccggc aaaacgcaat ccgttctcga tcatgccgct gaccaaagaa
     9241 acccgttccc tgctcgacat ggcctaccgt gtcggcgccc cccgcttcca cgaactcgcg
     9301 gtggcgcagg cgcgtcattc ggcgcagaag ctgcacttcg ccttccggcc cgaggcgccg
     9361 gcggtggcct ccaccgccga ggtgccgatc ccgcgccccg acacctcggc gctgctcgcc
     9421 cgcctgtacc ggcccttcgg cagccatgtg gacgaccgtc tgccgctggt gatcttcgcg
     9481 cacggtggag gctggtgcat cggcgacgtg gcgagctacg acgtggcctg ccgcgaactc
     9541 gccaacggct cgggttgcgc ggtgctgtcg gtggagtacc ggctcgcgcc cgagcatcct
     9601 ttccccgccg cgcccgagga catgcgcctg gccttcgatt ggtcggtcga caacgccgac
     9661 ctgctcggca tcgacccggc gcgcatcgcc ctcgccggcg acagcgccgg gggcaacctg
     9721 gcgatcgtca ccgcgctcgc cctgcgcgcg gccgaggtga agccggcctt cctgctgctg
     9781 atctacccga gcaccgagat ccgcagcgcg cggccctcgc gcgagcgcta cgccgagggc
     9841 ttcttcctcg atcgtgagtc gctgcagtgg ttcttcgagc gctacctgcc cggcgtcgat
     9901 accgaggact ggcgggtgtc gccgatgcgc gcggcctcgc tcgccgggct gccgccgatg
     9961 caggtgatct gcgcggaatg cgatccgctg gtggacgact gcctggcgtt cgtcgagcgc
    10021 gtgcgcagcg aaggcggcga ggtggcgctg cgtgtcttcg agggcgtggt gcatggcttc
    10081 tacaccctgg gcaagctcat cccggaggcg cgcgaggccg tggcgctgtc ggccgcggcc
    10141 ttgcgctccc acctcgcgga gtgcgcgcag tcctgagcgg atccggcagg cgggcgtccg
    10201 ccgggaacgg ccggccgcgg agttcagccg caggcggcgg agatcagctc ctgacggctg
    10261 gcgcgggctt cggacatcag ggtgtcgaag ccgtcgggat cgagtccgtc cgccacgctc
    10321 gcttcgagca gttcgccgcg ctggcggctg ccgagcaggg tgtcgaaggg gttgggcgat
    10381 tcggccgcga gggtggcgag gaagaccacg tcgccgagct cggccggcgg ccactcggtg
    10441 ttggcgaagg ggtcggcggc gcaggccgtg atgatgcgct cgggcatctc cagggcttcg
    10501 agcacggcgt cgcgcaccgg ctcgtcccag gtggtgacca cctcggcgaa gcggcccatg
    10561 tccccttcca gcgccgggta gcgcgccgcg cgcgcaagca ggtagaactg gccgatgtcg
    10621 agcatcaggc cggcgagcat cgcggcatcg gggtcggggc gcttgaggtg gtgtgcgaag
    10681 gcgcaggacc acgccgccac gtcgatcgag tgcatccaca ggccgctggc gatcaggcgc
    10741 atctggcgcg agcggtggtc gcgcgcgagc tgctcggcgg cgaccgcgaa ggcgaggcag
    10801 cgcagcgtgg acaggccgat gcgctggatc gcatcgctca ggctggtgat ctggccgcgg
    10861 taggggttca gcgtcagcgt gttggccatg cggatgacct tggccgcaag caggggctcg
    10921 gtctgcaccg cgcccgcgat ctgcgcgagg gaggtctccg gggcgtcagc cagcttctgg
    10981 acgcgcaccg acaggtcgaa tacggtggga aagctcgtca cgccttcgcg cagctcctgc
    11041 tgcacctgtt cggcgaaggc ttcgaattgg gtgtcgaaag agctcatggg ccggttccgt
    11101 tcgtgtgatc gtcgcagtct atgcgcatgc tggccggcgt gcgcgtgcat atcccacgaa
    11161 cgaccgtcgc ttcagggctg ggtgaggcgg acgtggccgt tctccagcgc ctcgacctgg
    11221 gcgagcgtgc acaccggcac gccgagggcc tcgatggcgg cgtggccgtg cttgaagcgc
    11281 ttctcgacga tgaagctcgc gcacagcacc tcggcgccgg cctcgcgcgc catctcgacc
    11341 agggccaccg cggtgcggcc gttggagagg aaatcgtcga cgatgacgac gcggtcggtg
    11401 ggcacgatgt agcgctgcga cagcacgagc ttggtctcgc cgcccttggt gggcgagggg
    11461 atcacgcgcg cgatcgcggg ggattcgacc tcgggcgcgt acttcttcgc atacaccagc
    11521 ggcacgtcga gggcctgggc gaccaccagt ccgggggcga tgccgctggc ctcggcggta
    11581 agcaccacgc tgggttcgaa gcgtgccagg cgcttcgcga gctcctggcc catctcggtg
    11641 atgaagctgg gttcgatgcg gtggttgagg aagtggtcga tcttgaggat ctggtcgccg
    11701 atgacggtgg cttcggcttc gatacggcgc acgagcgggc cgtaggggcg gatgaggtcg
    11761 ggatcgggaa gcatgcgcgg gtccgtaaac gggaacgccc ccgggccggg ggcaggacgg
    11821 gcggattcta gccgaaggca gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnccaggtcg acggcgcgtg cggctcggcc cgcggccttt cgggctagaa tcgcccccgt
    12001 tcccgatctg cgaaaaccat gaacgacgaa gactacatgc gcgctgcgct cgagcaggcg
    12061 cgcgcggccg gggccgcgga cgaggtgccg gtgggcgcgg tggtggtgtg cgggggcgag
    12121 atcgtcggcc gcggcttcaa ccagccgatc gggcgccacg acccgaccgc gcacgccgag
    12181 atcatggcgc tgcgcgacgc tgcggcgcac ctcggcaact accgcctgcc cggctgcgag
    12241 ctctacgtca ccctggagcc ctgcgcgatg tgctcgggtg cgatcatgca cgcccgcatc
    12301 gcgcgcgtgg tgttcggtgc gcgcgacccc aagaccggtg tcgccggcag cgtgctcgat
    12361 ctcttcgccg agccgcgcct caaccatcac gccaccatcg agggcggact gctcgccgac
    12421 gagtgcgggc gcatgttgtc gagcttcttc gccgcccgcc gcgggcgcac gctggtggcc
    12481 tgaatgcacc tccgcgtgga cctcggccgg cagtgcctgg agctcttcgg cgccgacggc
    12541 gcctgcatcc gccgctacgc ggtgtcgacc ggcgaacgcg gtgcgggcga gcgcagcggc
    12601 agcctgtgca cgccgcgcgg ccgccaccgt atccgtgcgc gcatcggcgc gggcgcaccg
    12661 gcgggtgcgg tgttccgcgg gcgcaggccg accggcgagg tgtggacggc ggagttcgcg
    12721 gccgcccacc cggggcgcga ctggatcctg tcgcgcatcc tgtggctgtg cggcgaggag
    12781 cctgggcgca accgcggtgg cgaggtcgac accatgcggc gctacatcta cctgcacggc
    12841 accggcgacg accagcccat gggcgttgcg cgctcgcacg gctgcgtgcg catgcgcaac
    12901 cacgacatca tcgagctgtt cgagctcgtg ccggcgggca cggtggtgga gatcgtcgaa
    12961 tgaagggcgt gcggatcgag gtggtgggct gggacgaggc cgaggcgcgg gtgatgccgg
    13021 tgcgtaccgg ggtcttcgtg gtggagcagg gcgtgcccga ggagatcgag cgtgacgcct
    13081 tcgacgcggt gtgccggcac gcgatcgcgc acgatgccga gggacgggtg atcgccaccg
    13141 ggcgcctgct gcccgacggc cacatcggcc gcatggcggt gctgcgtgag gcgcgcggcg
    13201 ccggggtggg cggggcggtg ctgcaggcgc tgatcgccga ggcggcgcgg cgcgggctga
    13261 acgaagtcgc gctcaatgcg cagacccacg cgctcgactt ctacctgcgc cacggcttcg
    13321 agccggtggg cgaggtcttc atggaagcgg gcattcccca ccgcgcgatg cggcggccgg
    13381 tgtagcggcc gcgcacgcga gcgcattgcg cgcggcgttc aggtcgtgcc ggcgccgaac
    13441 gcgtgctcga cccggctgcg cagttcccca tcggcgtccc aggtccattc gacgaggcgg
    13501 gtgcggccct tgcgatcgcg cagcaccacg ctgctgcagc gcgtgccgta gccgggtgcg
    13561 cggatgaagg cgggcgacag ccagcgctcc cactccaggc ttacgccggt cgcgggcagg
    13621 tgcgggtcgg gcgcgggcgt ggggtcgcgc agcaggccga ggaggccggc gacgagcgca
    13681 tcctccgtcg ccgcggccgg gcctgacgtc ggcagcgcct cgagcttgcc ggcgagtgct
    13741 gcgcgcgtct tcaccagctt gggccagggg ctgtcgagca agtggttgga caggccgtag
    13801 tatcccggct ccagcatgcg cagcccgccg gtggtgctct cgagcacgcc gagccggtcg
    13861 gcgtcgccca ccagcaggtt gaaggcggcg tagttgcccg cgctcgcctg cacggcctgc
    13921 agcgtggcgg gcgccgggcg gacatcctcg agcgcctcgc gcaccagggc gccgcgcgag
    13981 ggcgcgccgg cgatgtggcg gctggggtcg cggtagttgg tcagcgcggc gaagcggccg
    14041 ccgcgcgtaa ggcccatcca ggtgccgccg gcctcgaggt cgcggccggc gagcagctcg
    14101 ggtgcgtcca cccaccaatg cgcgggcgcg gcggggcgcg cgagatattc gtcgcggttg
    14161 gccgcgacca ccagcggata gtcgggatgc gcctgccagg cgaagacgat caggcacatg
    14221 gctacgcgcc tcggcgagta cgaccaggcc cccgggcccg gtcgcggtgc ccgtcaggcg
    14281 agcgcatacg gcaggtcgag caggtctgcg cgcggcccgt cgggcgcgcc gacacggatc
    14341 tcgccggctt ccgcgctgct cgactggatc accgcgagcg cctcgacgcc accgtcggcg
    14401 gtcggtgcga cgttgaccag cttgccggcg gactgctcgc cgaagtcggg cgcatagacg
    14461 tcggtgccgg gcgcggccgt gctgccggcg ggcagggcga ggcggtaggt gcgcttcttg
    14521 agcttgccga ggtactgggt gcgcgcgacg atctcctggc ccggatagca gcccttgtgg
    14581 aagtccaccc cgccgatgag ctcgtagttg agcatctggg cgacgaactc ttcctgggtg
    14641 ggcgcggtga ccagcgccag gccggcgcgg atcatgccga gctgccaggc cgcggtgccg
    14701 gcacgcagcg cgccggcggc gacgagcgcg tcgaacagcg ccggggcggc gtcggtggcg
    14761 acggcgagca ccaggcgctg cgcgccgagg cggatgcagc gcacgcccgc ggccgatacc
    14821 gcctggcgca tgtcctcggc gggcagcgcg gcgcctgccg cctgcgcgat cgccacggcc
    14881 tgcggaccgg cgaggccgat cagcgcgacc tcggcgctgg cgtcggcgag cttgaccttg
    14941 ctgcgcagca cgtacatcga gaacttcttc aggaacgcgg gcaggatgtc tgccgagagc
    15001 gcgagcgcat ggccgccctc ctcggtccac accaggaagc tggcgatcat ccgccccttg
    15061 ggcgaattga agctgttcca cgccgcggtg tcggcttcca tgtgcttgac gtcgttcgac
    15121 accaggttgt gcaggaaggc ggcggagtcc gggccctgcg agcggatcgt gcccaggtgc
    15181 agcagcggca ccgcgatggt ggcggtctcg gtcgcgcgcg cttcgtcggc gggcgaggcg
    15241 aaggcgatgg agcgggcggt ttcgtcgagc gtggcgccca tgcgggcgag gtgttcggtc
    15301 caggcagtca tggtctcggt tcgggcagtc tcggaaggga ttcgggggcg cgccgcagga
    15361 gggggagggc acgtgctgcg gtattatagg cgccctcccc gtggcggtcg cccctcgcgc
    15421 cgacagggat ttagccggct cttccccctc cgatgaagcg ttttctcccc cgcctgctcc
    15481 tgctgctcgc cgtgcttgcc atccttgccg cgatgatcgc ggcgacgggc tggtggtatg
    15541 cgcaccggcc gctcgcgctc gccgccgagc gggtggattt caccgtggcc cgcggcatgg
    15601 gcatgcgcca ggccgccgcc gccatcgagc gcgccggcgt gggcgtggat gcgcgcctgc
    15661 tcgcgctgct cgcgcgtctg acgaagcgcg acgcccgcat caaggccggc agctacgagg
    15721 tgcacgccgg catcacgccc tggcagctca tcctcaagct ctccgacggc gacgtcacgc
    15781 agggcgagct gttgctggtc gagggctgga ccttccgcca ggtgcgccag gcgctggagt
    15841 cccatccgga tctggaagcc gacaccgccg ggctgggcga ggcggagatc ctcgcgcgca
    15901 tcggcgcgag cgcgcagaac gccgagggcc tcttcttccc cgatacctat ctgttcgaca
    15961 agcgctcggg cgcgctcgcc gtgctgcgac gcgcgcacga ggccatgcag gcccgcctcg
    16021 acaaggcctg ggccgagcgc gacccggcca cgccgctggc ctcgccctac gaggcgctga
    16081 tcctcgcctc gatcgtcgag aaggagaccg gccgccccga ggaccgcgcc ctggtcgcct
    16141 cggtgttcgc caaccggctg cgcatcggca tgcgtctgca gaccgacccc acggtgatct
    16201 acggcctcgg ccccgagttc gacggccgcc tgcgccgggc gcatctcgat gccgaccacc
    16261 cgtggaacac ctacacccgt gccggcctgc cgccgacgcc gatcgcgatg ccgggcgagg
    16321 ctgcgctgcg cgctgcgctc aaacccgaga agagcgactt cctttatttc gtcgcgcgcg
    16381 gcgacggcag cagcgagttt tcgcgcgacc tcgcggcgca caaccgcgcc gtcgataaat
    16441 acatccgcaa cggaggtggg ggatgagccg gatgccgccg cgcctgcccg aaaccgacat
    16501 gatcgagaac gcaccctccg ccgtgcccgc gagcgcagcc gcgcccgccc gcggccgctt
    16561 catcaccttc gaaggcatcg acggtgccgg caagagcagc cagatcgcc
//
LOCUS       DAOQBS010000061        16565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2581_length_16565_cov_15.185342, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000061 DAOQBS010000000
VERSION     DAOQBS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 16565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16565
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2581_length_16565_cov_15.185342"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PKN25_08550"
     CDS             <1..526
                     /locus_tag="PKN25_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNV90988.1"
                     /translation="PAIDAEGRFLQLDFGKLSVVSLYLPSGSSSAERQQVKFDFMDRF
                     LPHMDQLFQSGQEVVVCGDWNIAHREADLKNWKSNQKNSGFLPEERAWLGRLFDEQGW
                     IDVYRRLHPEATADCYTWWSNRGQAWAKNVGWRLDYQVATPGLAASAQAAEVYKAQRF
                     SDHAPLTVDYDWTL"
     gene            672..1154
                     /locus_tag="PKN25_08555"
     CDS             672..1154
                     /locus_tag="PKN25_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90989.1"
                     /translation="MYNPDRRRFIARLGTGGLLLTPLAACSGEVWPEGMKPIAWDRDA
                     CVRCNMRIADHRFAAQLRGGPRDNTFTFDDVGCLVFWLEEQRTRFPWMDDVSTRMWVA
                     AWESPSVKAVTWLDPRQARYIVKRSPMRYDLAASAAPAADAHSLSYEEMRRKTLARGK
                     "
     gene            1168..1506
                     /locus_tag="PKN25_08560"
     CDS             1168..1506
                     /locus_tag="PKN25_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90990.1"
                     /translation="MPLERPSDRVLAATLLAGVLFAATFADAARQRSAREAALQRTTE
                     LVTGLRLSDVVLFTEARYTRHLSQADLHSAFQDHPMALEHFPSGSLLPPPRLLLDHHE
                     TLDRKAASPD"
     gene            1469..2605
                     /locus_tag="PKN25_08565"
     CDS             1469..2605
                     /locus_tag="PKN25_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNV90991.1"
                     /translation="MKPWIERQRHLIDFTAQSLARRKGKSLGLLFVYTLLVFVLASVA
                     LYTHALRNEATRVLAGAPEIVLQRLIAGRHDLVPPGYIERIGRIRGVQKIEGRLWGYY
                     YDSVLKANYTFMVPADREIVPGEIVVGPALERTRGLSAGNGISFRAYSGELHTFIVAA
                     VLAHESELVSADLVLMNEADFRRFFAYPDGHYTDIALWVANPLEVRNVGVKLLGTLPD
                     SRPILREEVLRTYASIFDWREGMMLALLSAAILAFGIFAWEKAAGLSAEEKREIGILK
                     AIGWETGDVIAMKFWEGFLVSLFAFLVGYVAAYVHVFHFEFTLFAPVLKGWAVLYPSF
                     ALTPQIDGLQVATLFVFTVLPYTAATLVPIWRAATTDPDTVMRS"
     gene            2610..3308
                     /locus_tag="PKN25_08570"
     CDS             2610..3308
                     /locus_tag="PKN25_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV90992.1"
                     /translation="MLELANIRKVFNQGQHNEYWALKGIDLEIPPARVSVLKGPSGSG
                     KTTLLTILGCLARPTEGRVRLNGEDISGLPERFLTEIRRRTFGFIFQQFNLIRGLSAV
                     ENIILPGYPGGTPRAQLLARAEALLADLQLAHRRDAKVEWLSGGEQQRVAICRALIND
                     PEVLVADEPTANLDSTLSAEFLAILRRLAEAGRTVILTSHDPLVVESEVVDRVYSLRD
                     GRLIEVVDTRKAWP"
     gene            3305..4315
                     /locus_tag="PKN25_08575"
     CDS             3305..4315
                     /locus_tag="PKN25_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV90993.1"
                     /translation="MIFQPAIIALLLAAGLSVAMLAVASPFALQLIRHWDLGSGSERQ
                     LRLERLTYLFSMMVALVCGLQVLSTLLFVFNADRMSVMFVGAMCAVGTLNANAYGFPA
                     LYAQLVAFFFAATWLAINRLDNQARDYPLTRIKYRLLLAYAPLAAAVLYLQFNYFIRL
                     EADVITSCCGSLFSEEADTVASEVSALAPARAMSVFYATLGGAILLAAWHWKSGGHRL
                     WSGLAVGTASVAAFFAALAGVVAFVSLYIYEHPHHHCPFCILKPEYGYQGYLLYIPLF
                     AAAAAGLAAGVLRMFGGRPSLATLAPQASARLAGIAAAGFVLFMLVASVMVLRSNLIL
                     LD"
     gene            4327..4830
                     /locus_tag="PKN25_08580"
     CDS             4327..4830
                     /locus_tag="PKN25_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNV90994.1"
                     /translation="MKNLLLVLACTLLAACGRGGSDAGPARGIAAPAFEAMGLDGGVR
                     RFPEDFADKPVIIDFWADWCRYCANSMTRIDRAWREHAASGLSVLAVNVAQDRATAAA
                     FIERLGVGYSAVLDPGARITRDYGVKGLPMTIFVDRNGLISGKVIGELDDRLLAIQLD
                     RILPETR"
     gene            4924..5535
                     /locus_tag="PKN25_08585"
     CDS             4924..5535
                     /locus_tag="PKN25_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNV90995.1"
                     /translation="MDRREALKMSVLASIGMATAGKAAAQSGCTGDGTPAQFIPKTAA
                     DPQPQVDDLAKHPKCPYCGMDRKQFHHSRMVVHYADDLADGTCSLHCAAISLALNIDR
                     DPKALWVGDNAAEGDTRPLVEVEKASFLVGSKLAGVMTARSKVAYGSAEAAKVSQAAN
                     GGELTDFDGALLAAYTDMSADVTRIRKNRAERRQKMMEQRNKG"
     gene            5532..6020
                     /locus_tag="PKN25_08590"
     CDS             5532..6020
                     /locus_tag="PKN25_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNV90996.1"
                     /translation="MKTLLAFVFAAWTGFALAQAPAAELPKPGTKDLCPVCGMLVSKY
                     PNWVATIVYKDGHAHFFDGAKDLFKFWHDPAKYAAGHSRDQIERISVTEYYGLTAIDA
                     RNAWYVVGSDVLGPMGHEFVPLASREDAADFLKEHKGARVLRFDEVGAGLPSMLDAGR
                     FE"
     gene            complement(6115..6801)
                     /locus_tag="PKN25_08595"
     CDS             complement(6115..6801)
                     /locus_tag="PKN25_08595"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNV90997.1"
                     /translation="MLKPLQGYAESKRDVRLQRVFGGAPGAVAWQNRGIFRERAWRRP
                     ERLRMKTLQGQASHRQGCTMIRKFAPILCLGLLATTVHAAEDIDTAALFSASLTGLDA
                     QPVAMERFRGRPLIVNFWARWCAPCREEIPELIRLRDAQGADGIEVIGVALESQVEGV
                     RVFATANDMRYPVVLAAKQGIPLMKALGNAAGGVPYTVYIGRDGKAVGSKLGIVRADD
                     LAAATKVLLK"
     gene            6913..7224
                     /locus_tag="PKN25_08600"
     CDS             6913..7224
                     /locus_tag="PKN25_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HNV90998.1"
                     /translation="MATTLQPSKDKIVDDLKSLISDAEELLKLTADQQGGKLDDLRAR
                     INGRVAVAKDRLADAEAAIVDSGKKAVRATDDYVHENPWQSVGVAAGVAFLLGLLVSR
                     R"
     gene            7229..7621
                     /locus_tag="PKN25_08605"
     CDS             7229..7621
                     /locus_tag="PKN25_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNV90999.1"
                     /translation="MSTEPRPPRPRLAESLHGIVDAGLQTAQTRLELLAVELQEEKLR
                     LTGLALNTVLAGLLLGFGLVFLMVFLTVLFWDEHRLLALGISTAICLGGGLLAASNAA
                     RAFRSGTKLFSASLAELARDRAALKKPD"
     gene            7635..8036
                     /locus_tag="PKN25_08610"
     CDS             7635..8036
                     /locus_tag="PKN25_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HNV91000.1"
                     /translation="MDPRQVELALRKQKLQLRAEAQRADITQRLAGIDGALDRVDALR
                     DHLAWAKDKVPVLSVALLVVLAARPRLTLRLAKRAWVGWLLLRQARGGRLSALLPTAV
                     PLLRGLLALLRRRLERSAGSGSAPPRRRRTS"
     gene            complement(7969..9216)
                     /locus_tag="PKN25_08615"
     CDS             complement(7969..9216)
                     /locus_tag="PKN25_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HNV91001.1"
                     /translation="MREIPIQSAEPLWRQAISGAQILFVAFGALVLVPLLTGLNPSLA
                     LLGAGVGTLLFQLLTGRQVPIFLGSSFAFIAPIIFSIQTWGIPATMGALACVSLMYFV
                     FAGLVWKHGAEIVHRYMPPVVVGPIIMIIGLSLAAVGVNMAMGRSGDGKLELVPYATA
                     LMVAGISFGTTVLVAVFARGFLKLVPILCGVAAGYVAAILFGLVDFAAVGAAPWFVLP
                     QFVTPSFELAAILFMLPVALAPAIEHVGDVLAIGGVTGKDYTEKPGLHRTLAGDGAGV
                     LFAGLVGGPPITTYSEVTGALTLTKNYNPAIMTWAAVFAVILAFFGKFNALLASIPVP
                     VMGGIMMLMFGSVAAVGLNTLIKARVDLEEPRNLVIVSSVLVMGIGGLALNFDGLTLQ
                     GVSLCAVVAVVLNLILPNARAGA"
     gene            complement(9337..9969)
                     /gene="upp"
                     /locus_tag="PKN25_08620"
     CDS             complement(9337..9969)
                     /gene="upp"
                     /locus_tag="PKN25_08620"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNV91002.1"
                     /translation="MPIHEIRHPLVRHKIGLMREGDISTKKFRELTAEIARLLAYEAC
                     QDFPLETVEIRGWAGPVEIEQIKGKKVTVVPILRAGLGMLDGVLDMVPSAKVSVVGIA
                     RDEETLQPVPYFEKFVGQLDERMALIIDPMLATGGSMVATVDMLKRKGCTHVRALVLV
                     AAPEGIALMDEKHPDVDIYTASIDSHLNEHGYIIPGLGDAGDKIFGTRHN"
     gene            10169..10726
                     /locus_tag="PKN25_08625"
     CDS             10169..10726
                     /locus_tag="PKN25_08625"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HNV91003.1"
                     /translation="MSLNLEEIKRAREEADCLADAATVEAALDRMAAEISARLADANP
                     LVYAVMNGGLILAGRILPRLPFPLEVAYLHATRYGHAIQGTLLDWRVRPTQDLRGRTV
                     LVLDDILDEGHTLEAIIAHLKEEGAAEVLSAVLVHKLHERKARPGMRADFTGLDIPDR
                     FLFGCGMDYKGYWRNAPGIYAVKGL"
     gene            10745..11092
                     /locus_tag="PKN25_08630"
     CDS             10745..11092
                     /locus_tag="PKN25_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HNV91004.1"
                     /translation="MLVTFKSHASADVIMFGDAAKKLVGILGKDPADAKGIVTVQQLP
                     EAIARLKAAIEEERARQAELERQRSHADEEAEKEAGNTGMAAPVGLAQRAWPLLAMLE
                     ESLREETPVVWGV"
     gene            complement(11117..12292)
                     /locus_tag="PKN25_08635"
     CDS             complement(11117..12292)
                     /locus_tag="PKN25_08635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HNV91005.1"
                     /translation="MPPRRQHYRGRNPARAHSIAELRAMAARRLPDFCLEYLDGGADD
                     ELALARNRRVLDEILLLPRTLVDVSQRELAVPLFGTDIALPAVIAPTGFNGLLTHAGD
                     RVLAEAAHAAGIPFCQSMVSTVALEDIAATGVRHWMQIYPFKDRDNLAAVVKRAEHAG
                     CEAIVLTTDASVFGNREWDRRNYRAPMKLAWRKLLDVGLHPRWVLDVLVPHGMPRFRN
                     LGDFLPPGMDSAKNAAAFLAAQMDTSLTWEDLRRLRDLWPRRLLVKGVLLPEDALRAQ
                     EAGADGVVVSNHGGRQLDCAPAPIETLAAVRQAVGAEMTVIVDSGFRRGSDFVKARAL
                     GADAAMSGRATLYGLAAAGAAGAARALEILRGEMERTLGLIGCARMDEVDARYVGRG"
     gene            complement(12412..12930)
                     /locus_tag="PKN25_08640"
     CDS             complement(12412..12930)
                     /locus_tag="PKN25_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HNV91006.1"
                     /translation="MRRLLLLLTPVLAATAHASPVPAEAPIRMAGLWLMNTTPVGAAA
                     QTSSFHICIGAPARDDVLAHPGSAVQNCREERWSKDEHYTYYQASCEAKGGSAAVEAR
                     FAGDFKYNFQGEVTMTIASQAGAAEVTRMEIDGRRLAPCRGGLPEGRFLIKGQDGVGN
                     LNLGEPIRQPAR"
     gene            complement(13034..13576)
                     /locus_tag="PKN25_08645"
     CDS             complement(13034..13576)
                     /locus_tag="PKN25_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV91007.1"
                     /translation="MTPDNTPCLLVVDDDPAFNRVLVRALGQRGFDTYGATDSEGALE
                     LARDHEPEFIVLDLNIGGESGLKLIRPLLEASPGARILVLTGYASIATAVDAVKLGAV
                     QYLAKPADIDSIIRALRAEGVRPEDHAPGAPLSVDRLEWEHIQRVLAEHHGNISATAR
                     ALQMHRRTLQRKLARPPAGA"
     gene            complement(13573..14901)
                     /locus_tag="PKN25_08650"
     CDS             complement(13573..14901)
                     /locus_tag="PKN25_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV91008.1"
                     /translation="MQPIEQETEPAEDLDNPNPVLMPARVLNAVRTDRGRLLQLRWVS
                     LLAMVLMSLVVFPWLAPAQPVGPLAGVTLCLLAVNLALLGGAARWLVGRWGAFLQLAV
                     DLLAWAAFLYFTGGVTNPAISLLLPVVAVGASILPAAQAWALAALAVALYSVLWQFHQ
                     PVRLADADQAMYWHLAGMWISFAVSAATVVWFIVRLNVELARRDNELAAVNAARARDT
                     YAVGLGKLAAGAAHRLGTPLGTLRILADDLASRPELASGLREDVQLMREQVDYCRDIL
                     NGLTREAGQQRAESGGAVEVRAWVTEVGGRWQRLRPGAGLHIDAEAADGQAVIVADAS
                     LGDALHNLIDNAANANERAGARACAVELGIRREGARVVVEVLDRGPGLPPERVEAARL
                     ARLGERAQGMGVGLMLAHAAVEHHGGHLEFRSRPGGGTIARMLIPRQSVR"
     gene            15113..>16565
                     /gene="nosZ"
                     /locus_tag="PKN25_08655"
     CDS             15113..>16565
                     /gene="nosZ"
                     /locus_tag="PKN25_08655"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
                     /protein_id="HNV91009.1"
                     /translation="MNDAKMNPVEAMPDPSRRKFLNTAALAGLAGAGMSVGLSACNKE
                     TAPAPAAAPAAAPAAAPAAAHAASGLKLHMAPGELDTYYGIWSGGHSGECRVLGLPSG
                     REIKRVPTFNIDCMSGWGITNESKKVIGTRADGQLKYKTGDTHHIHGSYTDGTYDGKY
                     FWVNDKIHGRLGRIRGDVFECDAITEIPNIQGFHGIFSDKRDPVDKAINHTTRVFCGN
                     EFHIPQPNDGRDLDDPTKYFCLFTCVDAETMEVRWQCKVDGNMDLVATSYDGRFAAAN
                     QYNTEGGARYEDMMSAEMDACVFFDVARIEKAIKDGKSFTVGTSKVPVVDGTKAANPD
                     PKTALTAYVPVPKNPHGVNASPDGKYMICSGKLSPTCTVIEVAKVADFLDGKLDDIRK
                     SVVAEVEVGLGPLHTTYDGRGNAFTTLFLDSQIVKWNIDAAIKFHNGDKAAQYVVDRI
                     DVHYQPGHISADMGETKEASGQFLAVGCKFSKDR"
BASE COUNT         2717 a         5913 c         5469 g         2466 t
ORIGIN      
        1 ccccgcgatc gacgccgagg gccgcttcct gcagctcgac ttcggcaagc tctcggtggt
       61 ttcgctctat ctgccctcgg gctcgagctc ggcggagcgc cagcaggtca agttcgattt
      121 catggaccgc ttcctgccgc acatggacca gctcttccag agcggacagg aggtcgtggt
      181 gtgcggcgac tggaacatcg cccaccgcga agccgatctg aagaactgga agtcgaacca
      241 gaagaactcc ggcttcctgc ccgaggagcg cgcgtggctc gggcgcctgt tcgacgaaca
      301 gggctggatc gacgtctatc gccgcctgca cccggaggcc accgcggact gctacacgtg
      361 gtggtcgaac cgcggccagg cgtgggcgaa gaacgtgggc tggcggctgg actaccaggt
      421 cgccaccccg ggactggctg cgagcgccca ggcggcggag gtctacaagg cgcagcgctt
      481 ctcggaccac gcgccgctga cggtggatta cgactggacg ctgtagcccc gtggggtgcg
      541 acacgcggtc gcacccctgc cctcctccgg ggccatgcgg cccgggggcc acgcgccggc
      601 gctatcatcc gggcattcga acccggcgtc cgcctccaag ggagatcctc gagcggtcgg
      661 agatgagccc catgtacaac cctgacagac gtcgcttcat cgcccgcctc ggcaccggcg
      721 gcctgctgct gacgccgctg gcggcctgct cgggagaggt gtggcccgag ggcatgaagc
      781 ccatcgcctg ggatcgcgat gcctgcgtgc gctgcaacat gcgcatcgcc gaccaccgct
      841 tcgccgcgca actgcgcggg gggccgaggg acaatacctt cacgttcgac gatgtcggct
      901 gcctggtctt ctggctcgaa gagcagcgca cgcgctttcc gtggatggac gacgtgtcca
      961 cccggatgtg ggtggcggcc tgggagagtc cgagcgtcaa ggcggtcacc tggctggatc
     1021 cgcgccaggc ccgctacatc gtcaagcgct cgccgatgcg ctacgacctc gctgccagcg
     1081 cagcgcccgc agccgatgcg cacagcctga gctacgagga aatgcgccgg aagaccctgg
     1141 cgcgcggcaa gtagcgggga ccttggcatg cccttggagc gcccctccga ccgcgtcctc
     1201 gccgccacgc tgctcgccgg cgtgctgttt gcggcaacct tcgccgacgc cgccaggcag
     1261 cgcagcgcac gcgaggcggc actgcagcgc acaacggaac tcgtcaccgg cttgcgcctg
     1321 tccgacgtgg tgctgttcac cgaggcacgc tacacccgcc acctgagcca ggccgacctg
     1381 cactccgcct tccaggacca cccgatggcg ctggagcact tcccctcggg ctcgctgctg
     1441 ccgccaccac gcctgctgct ggaccaccat gaaaccctgg atcgaaaggc agcgtcacct
     1501 gattgacttc accgcgcagt cgctcgcgcg gcgcaagggc aagagcctcg ggctgctgtt
     1561 cgtctatacg ctgctcgtgt tcgtgctcgc ctcggtggcg ctgtacaccc acgcgctgcg
     1621 caacgaggcc acgcgggtgc tggccggcgc gccggagatc gtgctgcagc gcctcatcgc
     1681 cggccgccac gacctggtgc cgccgggcta catcgagcgg atcggccgca tccgcggcgt
     1741 gcagaagatc gagggccggc tgtggggcta ctactacgac agcgtgctga aggccaacta
     1801 caccttcatg gttccggccg atcgcgagat cgtcccgggc gagatcgtcg tcggcccggc
     1861 gctggagcgc acgcgcgggc tgtcggcggg caacggcatc tccttccgcg catattccgg
     1921 cgagttgcac accttcatcg tcgccgcagt gctggcgcac gagtccgaac tggtcagcgc
     1981 cgacctggtg ctgatgaacg aggccgactt ccgccgcttc ttcgcctatc cggacggcca
     2041 ctacaccgac atcgcgctgt gggtggccaa cccgctcgag gtgcgcaacg tcggtgtcaa
     2101 gctgctcggc accctgcccg actcgcgccc gatcctgcgc gaggaggtgc tgcgcaccta
     2161 tgcgtcgatc ttcgactggc gcgagggcat gatgctggcg ctgctgtcgg ccgccatcct
     2221 cgccttcggc atcttcgcct gggagaaggc tgccggcctg tcggcggaag aaaaacgcga
     2281 gatcggcatc ctcaaggcga tcggctggga gaccggcgac gtgatcgcga tgaagttctg
     2341 ggaaggcttc ctggtctcgc tgttcgcctt cctggtcggc tacgtcgccg cctacgtgca
     2401 tgtgttccac ttcgagttca ccctgttcgc accggtgctc aaaggctggg cggtgctgta
     2461 cccgagcttc gcgctcacgc cgcagatcga cggcctgcag gtggccacgc tgttcgtgtt
     2521 caccgtgctg ccctacaccg cggccaccct ggtgccgatc tggcgcgcgg cgaccaccga
     2581 ccccgacacc gtgatgcgga gctgacgcga tgctcgaact cgccaacatc cgcaaggtgt
     2641 tcaaccaggg ccagcacaac gaatactggg cgctcaaggg catcgacctg gagatcccgc
     2701 cggcgcgggt gagcgtgctc aagggcccct cgggctcggg caagaccacg ctgctgacca
     2761 tcctcggctg cctcgcccgc cccaccgagg gccgcgtgcg cctgaacggc gaggacatct
     2821 ccggcctgcc cgagcgcttc ctgaccgaga tccgccgccg caccttcggc ttcatcttcc
     2881 agcagttcaa cctgatccgc ggcctgtcgg cggtcgagaa catcatcctg cccggctacc
     2941 ccggcggcac gccgcgcgcg cagttgctgg cgcgcgccga ggccctgctc gccgacctgc
     3001 agctcgcgca ccgccgcgac gccaaggtgg agtggctctc cgggggcgag cagcagcgcg
     3061 tggcgatctg ccgtgcgctc atcaacgacc ccgaggtcct ggtcgccgac gaacccaccg
     3121 ccaacctcga cagcacgctc tcggccgaat tcctcgccat cctgcgccgc ctcgccgagg
     3181 ccgggcgcac ggtgatcctc accagccacg acccgctggt ggtcgagtcc gaggtcgtcg
     3241 accgggtgta ttcgctgcgc gacggccggc tcatcgaggt cgtcgacacg cgcaaggcct
     3301 ggccatgatc ttccagcccg ccatcatcgc cctgctgctc gccgccggcc tgagcgtggc
     3361 catgctggcg gtggcgtcgc cgttcgcgct gcagctcatc cgccactggg acctcggcag
     3421 cggcagcgag cgccagttgc ggctcgagcg cctgacctac ctgttctcga tgatggtggc
     3481 gctggtgtgc gggctgcagg tgctgtcgac gctgctgttc gtgttcaacg ccgacaggat
     3541 gtcggtgatg ttcgtcggcg cgatgtgcgc cgtcggcacg ctcaacgcca acgcgtacgg
     3601 ctttccggcg ctatacgcac agctcgtggc gttcttcttc gcggcgacgt ggctggcgat
     3661 caaccgcctc gacaaccagg cgcgcgacta tccgctcacc cgcatcaagt accgcctgct
     3721 gctcgcctac gcgccgctcg ccgcggcggt gctgtacctg cagttcaact acttcatccg
     3781 cctcgaggcg gacgtcatca cctcgtgctg cggcagcctg ttctcggaag aggccgacac
     3841 ggtcgcgagc gaggtcagcg cgctggcgcc ggcacgggcg atgagcgtct tctacgccac
     3901 gctgggcggc gcgatcctgc tcgctgcctg gcactggaag agcggcgggc acaggttgtg
     3961 gagcgggctc gcggtcggca cggcctcggt cgccgccttc ttcgccgcgc tcgccggggt
     4021 ggtcgccttc gtgtcgctgt acatctacga gcatccccat caccactgcc cgttctgcat
     4081 cctgaaaccc gaatacggct accagggcta cctgctctac atcccgctgt tcgccgccgc
     4141 cgcggccggg ctggcggccg gggtgctgag gatgttcggc ggccgcccca gcctcgccac
     4201 cctcgccccg caggccagcg cacgcctggc cggcatcgcc gcggcgggct ttgtgctctt
     4261 catgctggtg gcgagcgtga tggtgctgcg ctccaacctg atcctgctcg actgaggcca
     4321 ctgccgatga agaacctgct gctcgtcctt gcctgcaccc tgctcgccgc ctgcggccgg
     4381 ggcggatccg acgccggccc ggcgcgcggc atcgctgccc ccgccttcga ggcaatgggc
     4441 ctggacggcg gcgtacgccg gttcccggaa gactttgccg acaagccggt gatcatcgat
     4501 ttctgggccg actggtgccg ctactgcgcc aacagcatga cgcggatcga ccgtgcctgg
     4561 cgcgaacacg cggcgagcgg actgagcgtg ctggcggtca acgtcgccca ggaccgcgcc
     4621 accgccgccg ccttcatcga gcgcctcggc gtcggttact cggccgtgct cgaccccggc
     4681 gcgcgcatca cccgcgatta cggcgtgaaa ggcctgccga tgaccatctt cgtcgatcgc
     4741 aacgggctga tcagcggcaa ggtcatcggc gaactcgacg acaggctgct ggccatccag
     4801 ctcgatcgca tcctgccgga gacgcgatga cccggccccg ggtcgcagcg cctgccgcag
     4861 gcgcagcccg attcgaatcg aaccctgccg gcacgcgccg gcaccacccc gaaggagcag
     4921 accatggatc gcagagaagc cctcaagatg tccgtcctcg ccagcatcgg aatggccacc
     4981 gccggcaagg ccgccgcgca gtccggctgc accggcgacg gcaccccggc gcagttcatc
     5041 cccaagaccg cagccgatcc gcagccgcag gtcgatgacc tcgccaagca tcccaaatgc
     5101 ccgtattgcg gcatggatcg caaacagttc caccactcgc gcatggtcgt gcactacgcc
     5161 gacgacctcg ctgacggcac ctgttcgcta cattgcgcgg cgatcagcct ggcgctgaac
     5221 atcgatcgcg accccaaggc gctgtgggtg ggcgacaacg cagccgaagg cgataccagg
     5281 ccgctggtcg aagtcgagaa ggccagcttc ctggtcggca gcaagttggc cggcgtgatg
     5341 accgcgcgca gcaaggtcgc ctacggcagc gccgaggccg ccaaggtctc gcaggcggcg
     5401 aacggcggcg agctcaccga cttcgacggc gcactgctgg ccgcctacac cgacatgtcg
     5461 gccgacgtca cccgcatccg caagaaccgc gccgagcgtc gacaaaagat gatggagcag
     5521 cgcaacaaag gatgaagacc ctgctcgctt tcgttttcgc agcctggacg gggttcgcgc
     5581 tcgcgcaggc gccggccgcc gagctgccga agccgggcac gaaggatctg tgcccggtgt
     5641 gcggcatgct ggtgtcgaag tatccgaact gggtggcgac catcgtctac aaggacggtc
     5701 acgcccactt cttcgacggc gccaaggacc tgttcaagtt ctggcacgac ccggcgaagt
     5761 acgctgccgg ccacagccgc gaccagatcg agcgcatttc ggtgaccgag tactacgggc
     5821 tgaccgccat cgacgcgagg aatgcgtggt acgtcgtggg ctcggacgta ctggggccga
     5881 tggggcacga gttcgtgccc ctggccagtc gagaggacgc ggccgacttt ctgaaggagc
     5941 acaagggcgc gcgcgtcctg cgtttcgacg aggtcggcgc gggccttccg tcgatgctcg
     6001 acgccgggcg cttcgagtag cccacggccc gggcgccgat ggcgcccggc gccgcccgtg
     6061 tctgagaact gcggcaacct ggccgcggca gtgcgggcat tcacccgcac gcgctcactt
     6121 cagcagcacc ttcgtcgccg cggcgagatc gtccgcgcgc acgatcccca gcttgctgcc
     6181 caccgccttg ccgtcgcggc cgatatagac cgtgtagggc acgccgcccg ccgcgttgcc
     6241 gagcgccttc atcagcggga tgccctgctt cgccgccagc accaccggat agcgcatgtc
     6301 gttggcggtg gcgaacacgc gcacgccctc gacctggctc tcgagcgcaa ccccgatcac
     6361 ctcgatcccg tccgcgccct gcgcatcgcg caggcggatg agctcgggga tctcttcgcg
     6421 acagggcgcg caccagcgcg cccagaaatt gacgatgagc ggccgcccgc ggaagcgctc
     6481 catggccacc ggctgagcgt cgagcccggt cagcgaggcc gagaacaagg ccgcggtgtc
     6541 gatgtcttcg gcagcgtgga cggtcgtcgc caaaaggccg aggcaaagga tcggggcgaa
     6601 tttacggatc atggtgcaac cttgtcggtg agaagcttgt ccttggagcg tcttcatccg
     6661 cagccgttcc ggtcggcgcc aggcacgttc gcgaaagatg ccccgatttt gccaagcaac
     6721 ggcacccggc gcaccgccga aaacacgctg caaccggaca tcgcgcttcg attcggcata
     6781 cccctgcagc ggcttcagca ctcgcttacg catcgttcat tgcggccgga cgccccgcgg
     6841 ggtaggcttg cttcaggcat ggcacgaagc gcttcaggcc acttttcccg gaccactcac
     6901 aggagaacca ccatggcaac gacccttcag ccctccaagg acaagatcgt cgacgacctc
     6961 aagtccctga tttcggacgc ggaggaactc ctcaagctca ccgccgacca gcagggcggc
     7021 aagctcgatg atctgcgcgc gcgcatcaac ggtcgcgtcg cggtcgccaa ggaccgcctg
     7081 gccgatgccg aggccgcgat cgtcgactcc ggcaagaagg ccgtgcgcgc aaccgacgac
     7141 tacgtgcacg aaaacccctg gcagtcggtc ggcgtcgccg ccggcgtcgc cttcctgctc
     7201 ggcctgctgg tcagccgtcg ctgattcgat gagcaccgag cccaggccgc ccagaccgcg
     7261 tctcgccgaa agcctgcacg gcatcgtcga tgccgggctg cagacggcgc agacgcggct
     7321 ggaactgctt gcggtcgagc ttcaggaaga aaagctccgc ctcaccgggc ttgcgctcaa
     7381 cacggtgctg gcggggctgc tgctcggctt cgggctggtg tttctgatgg tgttcctgac
     7441 cgtgctgttc tgggacgaac atcgcctgct cgcgctcggc atctccaccg cgatctgcct
     7501 cggcggcggg ctgctcgcgg cgagtaacgc ggcgcgcgcc ttccgtagcg gcacgaagct
     7561 gttctcggcc agcctggccg agctcgcccg cgaccgcgcg gccttgaaga aacccgactg
     7621 aaggcgtgcc gcgcatggat ccccgccagg tcgaactcgc gctgcgcaag cagaagctgc
     7681 agctgcgcgc cgaggcccag cgcgccgaca tcacgcagcg cctcgccggc atcgacggcg
     7741 cgctcgaccg cgtcgatgcg ctgcgcgatc acctcgcgtg ggcgaaggac aaggtccccg
     7801 tgctgtcggt cgcactgctc gtcgtgctcg ctgcaaggcc gcgactcacg ctgcggctcg
     7861 ccaagcgcgc ctgggtcggc tggctgctgc ttcgccaggc gcgcggcggg cggctctcgg
     7921 cgctgctgcc caccgccgtc ccgctcctgc gcggcctgct cgcgctgctc aggcgccggc
     7981 tcgagcgttc ggcaggatca ggttcagcac caccgcgacg acggcgcaca agctgacgcc
     8041 ctgcagggtc aggccgtcga agttcagcgc cagaccgccg atgcccatca ccagcaccga
     8101 agacacgatc accaggttgc gcggctcctc caggtcgaca cgcgccttga tcagggtgtt
     8161 cagcccgacg gccgccaccg agccgaacat cagcatcatg atgccgccca tcaccggcac
     8221 cgggatcgac gccagcagcg cgttgaactt gccgaagaag gcgaggatca ccgcgaacac
     8281 cgcggcccag gtcatgatcg ccgggttgta gttcttggtc agggtcagcg cgccggtgac
     8341 ctcggaatag gtggtgatcg gggggccgcc gaccaggccg gcgaacagca cgccggcgcc
     8401 gtcgccggcc agggtgcggt gcaggccggg cttctcggta tagtctttgc cggtcacccc
     8461 accgatcgcg agcacgtcgc cgacgtgctc gatcgccggc gccagggcca ccggcaacat
     8521 gaacaggatg gccgcgagct cgaagctcgg cgtgacgaac tgcggcagca cgaaccaggg
     8581 cgccgcaccc accgcggcga aatccaccag gccgaacagg atcgccgcga cgtagcccgc
     8641 cgccacgccg cacaggatgg gcaccagctt gaggaagccg cgcgcgaaca cggccaccag
     8701 cacggtggtg ccgaacgaga tccccgccac catgagcgcg gtggcgtagg gcacgagctc
     8761 gagcttgccg tcgccgctgc gccccatcgc catgttgacg cccaccgcgg caagcgacag
     8821 gccgatgatc atgatgatcg gccccaccac caccggcggc atgtagcggt gcacgatctc
     8881 ggcgccgtgc ttccacacca gcccggcgaa gacgaagtac atcagcgaca cgcaggcgag
     8941 cgcgcccatg gtggccggga tgccccaggt ctggatgctg aagatgatcg gcgcgatgaa
     9001 ggcgaagctc gagccgagga agatcggcac ctggcgcccg gtgaggagct ggaacagcag
     9061 ggtgccgacg ccggcgccga gcagcgcgag ggaagggttc agtccggtga gcagcggcac
     9121 cagcaccagc gcgccgaagg cgacgaagag gatctgggcg ccggagatcg cctgccgcca
     9181 tagcggctcg gcagactgga tgggaatttc acgcatgagg gttctccgcc gggtgcacgc
     9241 ggtcggcgtg gcgcgcagcc ggcatggttg tcgttatcga tgaagccgtg atgacgcggc
     9301 gaccgcagcg gacgatcgca cgccgtcacc gcggactcag ttgtgccggg tgccgaagat
     9361 cttgtcgccg gcatctccca gcccggggat gatgtagccg tgctcgttga ggtggctgtc
     9421 gatgctcgcg gtgtagatgt ccacgtcggg atgcttctcg tccatcagcg cgatgccctc
     9481 gggcgccgcc accagcacca gcgcgcgcac gtgggtgcag cccttgcgct tcagcatgtc
     9541 cacggtggcg accatggagc cgccggtggc gagcatcggg tcgatgatca acgccatgcg
     9601 ctcgtcgagc tggccgacga acttctcgaa gtagggcacc ggctgcaggg tctcctcgtc
     9661 acgcgcgatg ccgaccacgc tgaccttggc gctcggcacc atgtcgagca cgccgtcgag
     9721 catgcccagc ccggcgcgca ggatgggcac cacggtgacc ttcttgccct tgatctgctc
     9781 gatctcgacc ggaccggccc agccccggat ctcgaccgtc tcgagcggga agtcctggca
     9841 cgcctcgtaa gccagcaggc gggcgatctc ggcagtgagc tcgcggaact tcttggtgct
     9901 gatgtcgcct tcgcgcatca ggccgatctt gtggcgcacg agcggatggc ggatttcatg
     9961 gatgggcatg gcggtttccg gggcggcagg acgaagaccc gcctctttta ccgcgccgct
    10021 ggtccgcgca caagcgcaca aaccacagcc cgcggcatcg cggcaagccg ctcatccgcg
    10081 cacgccctcg aaggcggcgc cttgcgggga tcggcgctag aatgatgcgt ccgcaaacgc
    10141 accgggcgag cgcccgcagt tcatcgacat gtccctgaat ctggaagaaa tcaagcgcgc
    10201 ccgcgaagag gccgactgcc tcgccgatgc cgccaccgtg gaggccgcgc tcgaccgcat
    10261 ggcggccgag atcagcgcac gcctcgccga cgccaacccg ctggtctatg cggtgatgaa
    10321 cggcgggctg atcctcgccg gccgcatcct gccgcgcctg cccttcccgc tcgaagtcgc
    10381 ctacctgcac gccacgcgct acggccacgc catccagggc accctgctcg actggcgcgt
    10441 gcgtccgacc caggacctgc gcggacgcac ggtgctggtg ctcgacgaca tcctcgacga
    10501 aggccacacg ctcgaggcca tcatcgcgca cctgaaggaa gaaggcgccg ccgaggtgct
    10561 gtccgcggtg ctggtgcaca agctgcacga gcgcaaggcg aggcccggca tgcgcgccga
    10621 cttcaccggg ctcgacatcc ccgaccgctt cctgttcggc tgcgggatgg actacaaggg
    10681 ctactggcgc aacgcgcccg gcatctacgc cgtcaagggc ctgtaagaca aggagcgaca
    10741 ccccatgctg gtcaccttca agtcccacgc gagcgccgac gtgatcatgt tcggcgacgc
    10801 cgcgaagaag ctcgtcggca tcctcggcaa ggaccccgcc gacgccaaag gcatcgtcac
    10861 cgtgcaacaa ctccccgagg ccatcgcccg cctcaaggcc gcgatcgagg aggagcgcgc
    10921 ccgccaggcc gagctcgaac gccagcgcag ccacgccgac gaggaggccg agaaggaagc
    10981 cgggaacacc ggcatggccg ccccggtcgg cctcgcccag cgcgcgtggc cgctgctggc
    11041 catgctggaa gaatcgctgc gcgaggagac gccggtggtc tggggggtct gaggcacacg
    11101 cctccccacg aggccttcag ccgcgaccga cgtagcgcgc gtccacctcg tccatccgcg
    11161 cacagccgat cagcccgagc gtgcgctcca tctcgccgcg caggatctcc aacgcgcgcg
    11221 ccgcgcccgc ggcacccgcg gcggcaaggc cgtacagcgt cgcccgcccc gacatcgccg
    11281 catcggcacc gagcgcgcgc gccttgacga agtcgctgcc gcggcgaaag ccgctatcca
    11341 cgatcaccgt catctccgcc cccaccgcct ggcgcaccgc ggccagggtc tcgatcggcg
    11401 ccggcgcgca gtcgagctga cgcccgccgt ggttgctcac caccacgccg tcggcacccg
    11461 cctcctgcgc acgcagcgca tcctcgggca gcagcacgcc cttcaccagc agccggcgcg
    11521 gccacaggtc gcgcaggcgg cgcaggtcct cccaggtcag cgaggtgtcc atctgcgcgg
    11581 cgaggaaggc ggcggcgttc ttcgcgctgt ccatgcccgg cggcaggaag tcgccgaggt
    11641 tgcggaagcg cggcatgccg tgcggcacca gcacgtcgag gacccaacgc ggatgaaggc
    11701 cgacatcgag cagcttgcgc caggccagct tcatcggcgc gcggtagttg cggcggtccc
    11761 attcgcggtt gccgaacacc gaggcgtcgg tggtgagcac gatcgcctcg cagccggcgt
    11821 gctcggcccg cttcaccacc gcggcgaggt tgtcgcggtc cttgaaggga tagatctgca
    11881 tccagtggcg cacaccggtg gcggcgatgt cctccagcgc cacggtcgac accatgctct
    11941 ggcagaacgg gatgcccgcc gcgtgcgccg cctcggcgag cacgcggtcg cccgcgtggg
    12001 tgagcaggcc gttgaagccg gtgggcgcga tcaccgccgg cagcgcgatg tcggtgccga
    12061 agagcggcac cgcaagctcg cgctgcgaca catcgaccaa ggtgcgcggc agcagcagga
    12121 tctcgtcgag cacgcgccgg ttgcgcgcga gcgcgagctc gtcgtcggcg ccgccatcga
    12181 ggtattcgag gcagaagtcg gggagccggc gcgccgccat cgcgcgcagc tcggcgatgc
    12241 tgtgggcgcg cgccgggttg cggccgcggt aatgctgtcg tcgcggaggc acgtcgtgat
    12301 cgctccgtgg ggaggcggtg caggacccgg cgaggtcgtc cgctgcccgc tcggtgatct
    12361 gcggaccgtg gctgtgacga ccgactcccg tggtcggcaa cggctcgtgc ttcagcgcgc
    12421 cggctggcga atcggttcgc ccagattcag gttgcccacc ccatcctgcc ccttgatcag
    12481 gaacctgcct tccggcaggc caccgcgaca aggcgccagc cgccgcccat cgatctccat
    12541 cctggtcacc tcggcagcgc cggcttgcga cgcgatcgtc atggtgacct ccccctggaa
    12601 gttgtacttg aagtcaccgg caaacctcgc ctcgaccgcc gcgctgccgc ccttggcctc
    12661 gcagctcgcc tgatagtagg tgtagtgctc gtccttgctc cagcgctctt cgcggcagtt
    12721 ctgcacggcg ctgccggggt gggcgagcac gtcgtcgcgc gcaggcgcgc cgatgcagat
    12781 gtggaagctg gaggtctgcg ccgcggcccc gacgggggtc gtgttcatca gccacagacc
    12841 ggccatgcgg atcggcgcct cggcaggcac ggggctggca tgtgcggtgg cagcgaggac
    12901 gggcgtcagc agcaggagca agcggcgcat gatgggatcc ttgtgggggt ctgggggagg
    12961 ttcgatcggg aacgaaggtt agagcggaag gccgtccttg aacagagggg aaccggcgcg
    13021 acccgcctgc cggtcaggcc cccgccggcg ggcgcgcgag cttgcgttgc agcgtgcgcc
    13081 ggtgcatctg cagcgcacgc gcggttgccg agatgttgcc gtggtgctcc gcgagcacgc
    13141 gctggatgtg ttcccactcg aggcggtcga ccgaaagcgg agccccgggc gcgtgatcct
    13201 cgggtctcac gccctcggcg cgcaaggcgc ggatgatgct gtcgatgtcg gccggcttgg
    13261 ccaggtactg gacggccccc agcttcaccg catcgaccgc cgtggcgatg ctggcgtaac
    13321 cggtgagcac gaggatacgc gccccggggc tcgcctcgag cagggggcgg atgagcttca
    13381 ggcccgactc gccgccgatg ttgaggtcga gcacgatgaa ctcgggctcg tggtcgcggg
    13441 cgagctcgag cgcgccctcg ctgtcggtcg ccccgtaggt atcgaagcca cgctggccga
    13501 gggcgcgcac gaggacgcga ttgaaggctg gatcgtcatc gacgacaagg aggcagggcg
    13561 tgttgtcggg ggtcatcgta cggactgtcg cggaatcagc atgcgcgcaa tcgtgccacc
    13621 acccggtctg gaacggaatt caagatgccc gccatgatgc tcgacggcgg cgtgcgccag
    13681 catcaagccg acgcccatgc cttgcgcacg ctccccgagc cgcgccagac gcgccgcctc
    13741 cacgcgctcc ggcggcaacc cgggcccgcg atccagcacc tcgacaacca cccgcgcgcc
    13801 ctcgcggcgg atcccgagct ccaccgcgca agcgcgcgct cccgcgcgct cgttggcatt
    13861 ggcggcgttg tcgatgaggt tgtgcagggc atcgccgagc gatgcgtcgg cgacgatgac
    13921 cgcctgcccg tccgccgcct ccgcatcgat gtgcaagcct gccccggggc gcagacgctg
    13981 ccagcgcccg cccacctccg tcacccaggc ccgcacctcg accgccccgc cgctctccgc
    14041 ccgctgctgg ccggcctcgc gcgtgagccc gttgagaatg tcgcggcagt agtcgacctg
    14101 ctcgcgcatc agctgcacgt cttcacgcag cccggaggcg agctcgggtc ggctcgccag
    14161 atcgtcggcg agaatgcgca gcgtgccgag cggtgtgccg aggcggtggg cggcaccggc
    14221 ggcgagcttg cccagtccga ccgcgtaggt gtcgcgcgca cgcgccgcgt tgaccgccgc
    14281 gagttcgttg tcgcgccgcg ccagctccac gttgagccgc acgatgaacc agaccacggt
    14341 ggcggcggag accgcgaagc tgatccacat gccggccagg tgccagtaca tcgcctgatc
    14401 ggcgtcggcc aggcgaaccg gctggtgaaa ctgccacagc acggaataca gcgcgaccgc
    14461 gagggccgcc agcgcccagg cctgtgccgc gggcaggatc gaagcgccca ccgccaccac
    14521 cggcagcagc agcgagatcg ccgggttggt cacgccaccg gtgaaataga ggaatgccgc
    14581 ccatgccagc agatccacgg cgagttgcag gaaggcgccc cagcgcccca ccagccaacg
    14641 tgcagcccca ccgagcaggg cgaggttgac cgcgagcagg cacaaggtga ccccggcgag
    14701 cggcccgacc ggctgcgcgg gcgcgagcca ggggaagacg accaggctca tcagtaccat
    14761 cgcgagcagg gaaacccagc gcaactgcag caggcgcccg cgatcggtgc gcacggcgtt
    14821 gagcacccgc gcaggcatca gtaccggatt ggggttgtcg agatcctcgg cgggctcggt
    14881 ttcctgctcg atcggctgca ttcttccctt cgcaaacaag aactgaagga ctcatctttg
    14941 ccttatatca aggtttactg atttccacct tcatagaatt ggggtgcgac aaaacgtcac
    15001 aggacaattt gtcacaggcc cccgacacgc atgaagaacc gacgcaaggg gcgcaaggtc
    15061 gcgatgccgg gtctgtgaga tcgactacac ctgcctacgg gagcaaagaa ccatgaacga
    15121 cgcaaagatg aacccggtcg aagccatgcc cgatccgagc cgccgcaagt tcctcaacac
    15181 cgcagcgctc gccggcctcg ccggtgccgg catgtcggtg ggcctgtccg cgtgcaacaa
    15241 ggaaaccgct cccgctccgg ccgctgcacc cgccgcggca ccggccgccg cgcccgccgc
    15301 ggcgcatgct gcctccggcc tcaagctgca catggcaccg ggtgagctcg acacctacta
    15361 cggcatctgg tccggcggcc actccggcga atgccgcgtc ctcggcctgc cttcggggcg
    15421 tgaaatcaag cgcgtgccga ccttcaacat cgactgcatg agcggctggg gcatcaccaa
    15481 cgagtccaag aaggtgatcg gcacgcgtgc ggacggccag ttgaagtaca agaccggcga
    15541 cacccaccac atccacggct cctacaccga cggcacctac gacggcaagt acttctgggt
    15601 caacgacaag atccacggcc gcctgggccg tatccgtggc gacgtcttcg aatgcgacgc
    15661 gatcaccgag atcccgaaca tccagggctt ccacggcatc ttctcggaca agcgcgaccc
    15721 ggtcgacaag gccatcaacc acaccacccg cgtgttctgc ggcaacgagt tccatatccc
    15781 gcagccgaac gacggccgcg acctcgacga tccgaccaag tatttctgcc tgttcacctg
    15841 cgtcgacgcc gagacgatgg aagtgcgctg gcagtgcaag gtcgacggca acatggacct
    15901 ggtcgcgacc tcctatgacg gccgcttcgc cgccgccaac cagtacaaca ccgagggcgg
    15961 cgcgcgctac gaagacatga tgtcggccga gatggacgcc tgcgtgttct tcgacgtcgc
    16021 ccgcatcgaa aaggcgatca aggacggcaa gtccttcacc gtcggcacct ccaaggtccc
    16081 ggtcgtcgac ggcaccaagg ccgccaaccc cgatccgaag accgcgctga ccgcctatgt
    16141 accggtcccc aagaacccgc acggcgtcaa cgccagcccg gacggcaagt acatgatctg
    16201 ctcgggcaag ctgtcgccca cctgcacggt catcgaagtc gccaaggtcg ccgacttcct
    16261 cgacggcaag ctcgacgaca tccgcaagtc ggtggtcgct gaagtcgaag tcggcctcgg
    16321 ccccctgcac accacctacg acggccgcgg caacgccttc acgaccctct tcctggacag
    16381 ccagatcgtc aagtggaaca tcgacgccgc gatcaagttc cataacggcg acaaggccgc
    16441 gcagtacgtg gtcgaccgta tcgatgtgca ttaccagccc ggccacatca gcgccgacat
    16501 gggcgaaacc aaggaagcca gcggccagtt cctggcggtg ggctgcaagt tctccaagga
    16561 ccgct
//
LOCUS       DAOQBS010000062        16441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2614_length_16441_cov_13.901379, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000062 DAOQBS010000000
VERSION     DAOQBS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 16441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16441
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2614_length_16441_cov_13.901379"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="PKN25_08660"
     CDS             complement(<1..145)
                     /locus_tag="PKN25_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNV91010.1"
                     /translation="MKPFTQLDGLVAPLDRANVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRY"
     gene            complement(145..273)
                     /locus_tag="PKN25_08665"
     CDS             complement(145..273)
                     /locus_tag="PKN25_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HNV91011.1"
                     /translation="MKKLALIAAALVAGFVSMACNTVQGVGKDIERGGEAIQRAVK"
     gene            complement(302..1708)
                     /gene="leuC"
                     /locus_tag="PKN25_08670"
     CDS             complement(302..1708)
                     /gene="leuC"
                     /locus_tag="PKN25_08670"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNV91012.1"
                     /translation="MKAQTLYEKLWSSHVVHEEADGTALIYIDRHLVHEVTSPQAFEG
                     LKLAGRKPWRIGSIVATADHNTPTDHWDLGIQDPVSRQQVETLDANIREVGSLAYFPF
                     KDARQGIVHVIGPENGATLPGMTVVCGDSHTSTHGAFGCLAHGIGTSEVEHVLATQCL
                     LQKKSRTLLIRVDGQLGRGVTAKDVVLAIIGRIGTAGGTGYAMEFGGSAIRALSMEGR
                     MTICNMAIEAGARAGLVGVDETTIEYLKDRPFSPKGAQWEQAVDYWRSLHSDEGAEFD
                     KIIELKAEDILPQVTWGTSPEMVTTVDGRVPDPAAVTDPVRREGIERALKYMGLEANT
                     PITDIPVDQVFIGSCTNSRIEDLREAAAVAKGRSKAASVKRVLVVPGSGLVKRQAEAE
                     GLHEIFLAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSP
                     AMAAAAAVTGRFTDVRAL"
     gene            complement(1785..2600)
                     /locus_tag="PKN25_08675"
     CDS             complement(1785..2600)
                     /locus_tag="PKN25_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV91013.1"
                     /translation="MQSIFKIALPVLLAGTIATACQTVQTTHGGAVGVQRSQLMMVSA
                     QEVEQASNQQYQQMVAEARSKNALDRDPATVQRVRRIVSRLAAQTGAFRTDAPSWSWE
                     VHVFSSSELNAWCMAGGKMAIYTGLIERLALTDDEIAAVMGHEIAHALREHARERVSK
                     SMATGLGISVAGALLGVGQLGQDLMGQVAKVTFELPNSREHETEADRMGVELAARAGY
                     DPRAAVTLWNKMAGQSAGAPPQWLSTHPSHATRQRDLAEYAARVIPLYEGARR"
     gene            complement(2608..3777)
                     /locus_tag="PKN25_08680"
     CDS             complement(2608..3777)
                     /locus_tag="PKN25_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV91014.1"
                     /translation="MIPYWRLSAYYFFYFAFVGAFSPYFTLYLQSIALSATDIALLMS
                     LMQLMRVLAPNLWGWLAERLGMRIAIVRLSALASLAGFSVFFLTTEFAGLFAAMALMA
                     FFWSAVLPLIEGLAFAHLGEASHRYGRIRVWGSVGFIVAVLALGHSLDRLPIEAVLWI
                     TMSILVGIVLCSFIVPEAPRPPLQRDAASFGDTLRRPEVRALLGACFLMSAAHGALYV
                     FYSIHLVGMGYDKGVVGWMWTLGVLAEIGVFMWMPRISVRFSLRAILLFSFACAVARF
                     LMIGWGAHSLALLLLAQVLHGATFGAYHAAAIAVVNEWFPGRLQSRGQALYGSISFGA
                     GGMLGGLLSGYTWEGIGPAWTYTIGSGFALAGLLWLLLGWKGEPRPAHALHEPRR"
     gene            complement(3865..4995)
                     /gene="aroC"
                     /locus_tag="PKN25_08685"
     CDS             complement(3865..4995)
                     /gene="aroC"
                     /locus_tag="PKN25_08685"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNV91015.1"
                     /translation="MSGNTLGTLFTVTSFGESHGPAIGCIVDGCPPGLAICEADIQAE
                     LDRRKPGTSRHVTQRREPDTVEILSGVFEGVTTGTPISLLIRNQDQRSKDYGNIADTF
                     RPGHADYAYLQKYGLRDHRGGGRSSARETAVRVAAGAIAKKWLKERHGIVIRACMGAL
                     GPIEIPFVSWDEVDGNPFFAPNAAIVPELEAYMDALRKSGDSIGARIDVVASGVPVGW
                     GEPVYGRLDADIAYAMMGINAVKGVEIGAGFKSVAQRGTEHGDEMTPAGFLSNHAGGV
                     LGGISTGQDILASIAIKPTSSIRLERRSIDRAGNPVMVATEGRHDPCVGIRATPIAES
                     MLALVLIDHALRHRAQCGDVRTDTPRIPALAPAGSQRLPSPR"
     gene            complement(5091..5408)
                     /gene="ilvN"
                     /locus_tag="PKN25_08690"
     CDS             complement(5091..5408)
                     /gene="ilvN"
                     /locus_tag="PKN25_08690"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNV91016.1"
                     /translation="MMIEQVSDPLPQAGYAKVVLELDVNNHPGVMSHICNLFARRAFN
                     VEGILCMPVSDGKRSRIWLLVFEDQRLEQMVRQLEKLEDVLNVRRHGAEHEVFERLEG
                     FFH"
     gene            complement(5408..7075)
                     /gene="ilvB"
                     /locus_tag="PKN25_08695"
     CDS             complement(5408..7075)
                     /gene="ilvB"
                     /locus_tag="PKN25_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HNV91017.1"
                     /translation="MMQMTGAQLMVRLLERQGVRTVAGIPGGAILPFYDALSGSEHIH
                     HVLARHEQGAGFIAQGMARVSGVPQVCIASSGPGATNLVTAIADACLDSIPMVVITGQ
                     VPQAMIGTDAFQEVDIYGITVPITKHNFLVRSAQELLEVIPDAFRIAMSGRPGPVLID
                     VPKDVQNQLVAVAEFPAPAVADPAPQLDLAAVELAAKMINEAEQPVLYLGGGVVASGA
                     APLAVQLAEQAGLPTTMTLMALGAMPMDHPLSIGMLGMHGARYTNFVLEEADVLVCVG
                     ARFDDRAIGRAAQFCPNAKIVHIDIDRSELHKIKNAHVAIHANVKCALEALLPRVQAT
                     LRKRWLSHVDSLKTRFPMQFPGQDDPRTHYGLIQAVAAALDDRAIVATDVGQHQMWVA
                     QAYPFRRPRQWLTSGGLGTMGFGLPSALGAALAEPGRTVVCFTGDGSFKMNIQELATL
                     AEEGLNVKIVLMNNNALGLVYQQQTLFYGQRLFASKYRTEPDFVKIAEGFGVPAVDLD
                     LADNPRAALAEALHRPGPCLIHATIDREQFVYPMVPPGAANTEMIGG"
     gene            complement(7404..9065)
                     /locus_tag="PKN25_08700"
     CDS             complement(7404..9065)
                     /locus_tag="PKN25_08700"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HNV91018.1"
                     /translation="MEKIWLKSYPPGIPAEIDVDEFRSLGDLFERSVARYADKTAYHC
                     MGKSITYAELDALSARFGAWLQGALKLPRGARVALMMPNVLQYPIALFGTLRAGYTVV
                     NVNPLYTARELEHQLKDSGAEVIVILENFAHTLEHVRAQVPIRHIVVTSLGEMLGLVK
                     GAIVNFVVRKVRKLVPPWRLDGHVRFSTALQRGAAHELQPAQVGHEDVAFLQYTGGTT
                     GVAKGAMLTHRNVIANLQQAHAWIAPYVGEGKEIIITALPLYHIFSLTANCLTFLKIG
                     AKNVLIPNPRDIPGFIKELGLHRFTAITGVNTLFNALLNNPEFAKLDFSTLRVSLGGG
                     MAVQQVVAEKWKKVTGVSLVEAYGLTETSPAVTINPLDLAEFNHSIGLPVSSTEISIR
                     DDAGHEVPLGQRGELCVRGPQVTPGYWQRPEETARIFTHDGFLRTGDIATVDERGFVR
                     LVDRKKDMILVSGFNVYPNEVEDVVAAHPGVLEVAAIGVPDERSGEAVKVFVVRKDPK
                     LTAEALIAFCRENLTGYKVPHHVEFRDELPKSNVGKILRRELRPK"
     gene            complement(9279..9728)
                     /locus_tag="PKN25_08705"
     CDS             complement(9279..9728)
                     /locus_tag="PKN25_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV91019.1"
                     /translation="MLVSEILAIKGKVLFTIGPNRPIAEAVEIMNEQDVGSLVVFSRG
                     EMVGMLTFRQVLQAVQQGGADWQSLQVEAVMLREPRVASPDMEMDELRRLMVEHHQRY
                     LPVMDGNTLLGVVSFHDVAKAVLEEQSFENRMLKNYIRNWPAGEGEE"
     gene            complement(9821..10144)
                     /locus_tag="PKN25_08710"
     CDS             complement(9821..10144)
                     /locus_tag="PKN25_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNV91020.1"
                     /translation="MTYVVTERCIRCKYTDCVDVCPVDCFREGPNFLVIDPEECIDCT
                     LCVAECPVEAIYSEDDVPDDQKGYIALNAELAKQWKPIVERKDPLPDADHWARIKGKL
                     GELKR"
     gene            10519..10845
                     /gene="trxA"
                     /locus_tag="PKN25_08715"
     CDS             10519..10845
                     /gene="trxA"
                     /locus_tag="PKN25_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HNV91021.1"
                     /translation="MSEHIHHVTDGNFDDEVLQSQTPVLVDYWAEWCGPCKMIAPILD
                     DVAKDYAGKLKVAKLNIDDNQETPAKYGIRGIPTLMLFKGGNVEATKVGALSKSQLTA
                     FIDSNI"
     gene            11024..12283
                     /gene="rho"
                     /locus_tag="PKN25_08720"
     CDS             11024..12283
                     /gene="rho"
                     /locus_tag="PKN25_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNV91022.1"
                     /translation="MSELKALHVSQLLQMAMDAGVEGANRLRKQELVFALLRNRARRG
                     EPICGDGALEVLPDGFGFLRSPDTSYLAGTDDIYVSPSQIRRFNLRTGDTIEGEIRTP
                     KDGERYFALTKLDKINGRPPEECKTKILFENLTPLHPQECLKLEREVRGEENVTSRII
                     DMIAPIGKGQRGLLVAPPKSGKTVMLQHIAHAITANHPDVKLIVLLIDERPEEVTEML
                     RSVKGEVVASTFDEPATRHVQVAEMVIEKAKRLTEHKYDVVILLDSLTRLARAYNTVV
                     PASGKVLTGGVDANALQKPKRFFGAARNIEEGGSLTIIATTLVDTGSRMDDVIYEEFK
                     GTGNMELHLDRRMAEKRVYPAINVNRSGTRREELLLKADVLQKVWILRKLLYGMDDID
                     AMEFLLDKVKATKSNAEFFDAMRTGRG"
     gene            complement(12360..13211)
                     /locus_tag="PKN25_08725"
     CDS             complement(12360..13211)
                     /locus_tag="PKN25_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNV91023.1"
                     /translation="MSIGKIGVVGAGTMGNGITQAFAVAGRKVVMMDVAEAQVQRGLA
                     TISASLDRLIKKDRMSAPQKDAAMACITTVSSLDALADCDLVIEAATENVDLKLRIFA
                     DLDRIVKPEAILASNTSSISITRLAAGTQRPGQVIGMHFFNPVPVMALVELINGLQTS
                     AATYSAVEDVAKAIGKTPIKVKNSPGFVVNRMLCPMINEAVFALGEGLATAAEIDEAM
                     KLGCNHPIGPLALCDLIGLDVELAVMQVLYEGTKDPKYRPAPLLVEMLEAGYLGRKSG
                     KGFYDYK"
     gene            13513..14226
                     /locus_tag="PKN25_08730"
     CDS             13513..14226
                     /locus_tag="PKN25_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNV91024.1"
                     /translation="MLHTSPPYAVRQETEVAEPKTLVEAAYRSLRRNIIEGRLSPGEK
                     LRIEHIKNDYGVGGATLREALSLLTSDALVVSQGQRGFRVAPVSLEDLADITETRVML
                     ECSALRQSITLGDEAWEAELTAAFHRLTRAEEKLAEGNNREQWEERNRIFHEVLIAAC
                     PSRWTKHFLSILYHQAERYRHLSLRRSDGTRRLHDEHAALFEAAMGRDAETATRLLGE
                     HIRLTLQSVSLDKAATASH"
     gene            complement(14429..15004)
                     /locus_tag="PKN25_08735"
     CDS             complement(14429..15004)
                     /locus_tag="PKN25_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV91025.1"
                     /translation="MSNKSLDIIHDEHRALAAMLSGLRGLVASIEAGRLKPDFELMAS
                     MIEYIEMVPEKVHHPKEDSYLFARLRERSAEALPIIERLEAEHREGDARMATLRAALE
                     AYRREGESGLAGFQGALKTYIEHEWQHMNTEEHLIFPLARKHLSAEDWAHIDAAFLAN
                     DNPWQGPAGKYAALFTRIVNTAPAPVGLGGG"
     gene            complement(15025..15738)
                     /locus_tag="PKN25_08740"
     CDS             complement(15025..15738)
                     /locus_tag="PKN25_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV91026.1"
                     /translation="MSNMLLEAKEIHTYYGASHILHGIDFNIREGEGIALMGRNGMGK
                     STLIKSMLGIVRPRHGRVLVRGDDYTRARTFQTAQQGIAYVPEGRGIFKSLSVRENLL
                     MSARAGVDGRREWTFDRVMETFPRLGERINNGGWQLSGGEQQMLTIGRALMTNPDLLI
                     LDEATEGLAPLIAMEIWRIVKEIRKTGIATVIVDKNHGAVTSICDRAMILVKGQVVFD
                     GTSDEIRAQPELIQKHLGV"
     gene            complement(15731..>16441)
                     /locus_tag="PKN25_08745"
     CDS             complement(15731..>16441)
                     /locus_tag="PKN25_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV91027.1"
                     /translation="LVANQDISVTLERQMIHVLLGPNGAGKSTCINMLSGDLPPSEGR
                     IHYLGRDITALTAAQRSQVGIGRSYQRTNIFPQFTVLENVRLAAQSRRQQPWRLFSKA
                     MEQKWALEKARACIEEAGLGKRVDWVSGVLSHGEQRQLEIAMVLATDAQVLLLDEPLA
                     GMGSEESASMVEVLKRLRQTRGILLVEHDMDAVFAVADMITVMVNGQLLECGPPAQIK
                     ASVAVQQAYLGTEDSFHV"
BASE COUNT         2657 a         5587 c         5524 g         2673 t
ORIGIN      
        1 ggtagcgcca ctcgtcgaag gcgttggggc cgaagccgct gcgcttgatc gacttgagga
       61 actgcttggg gatgatcgcg tcggtatcga cgttggcgcg gtcgagcggc gcgaccagcc
      121 cgtcgagctg ggtaaacggc ttcattattt gactgccctc tggatggcct cgccacccct
      181 ttcgatgtcc ttgccgacgc cctgcacggt gttgcaggcc atggagacga agccggcgac
      241 gagcgccgcg gcgatcagtg cgagtttctt cattgccttc tcctctgtcc ggaacgcccg
      301 ctcagagcgc gcgcacgtcg gtgaagcggc cggtgaccgc ggcggccgcg gccatcgccg
      361 ggctgaccag gtgggtgcgc ccgcccgcgc cctggcggcc ctcgaagttg cggttcgagg
      421 tcgaggcgca gcgctcgcct ggctcgaggc ggtcggcgtt catcgccagg cacatcgaac
      481 agcccggctc gcgccactcg aagccggcgg cgaggaagat ctcgtgcagg ccctccgcct
      541 cggcctggcg cttgaccagg ccggagcccg gcaccaccag cacgcgcttg acgctggcgg
      601 ccttgctgcg gcccttggcc accgcggcag cctcacgcaa atcctcgatg cgcgagttgg
      661 tgcacgagcc gatgaagacc tggtcgaccg ggatgtcggt gatcggggtg ttggcctcga
      721 ggcccatgta cttgagcgcg cgctcgatgc cctcgcggcg caccgggtcg gtgacggcgg
      781 cgggatcggg cacacggccg tcgaccgtgg tgaccatctc gggcgaggtg ccccaggtga
      841 cctgcggcag gatgtcttcg gccttgagtt cgatgatctt gtcgaactcg gcgccctcgt
      901 cgctgtgcag gctgcgccag tagtcgaccg cctgctccca ctgcgcgccc ttgggcgaga
      961 aggggcgatc cttcaggtat tcgatggtgg tctcgtccac gccgaccagc ccggcgcgcg
     1021 cgcccgcctc gatcgccatg ttgcagatcg tcatgcggcc ttccatcgac agcgcgcgga
     1081 tggcgctgcc gccgaactcc atcgcgtagc cggtgccgcc ggcggtgccg atcctgccga
     1141 tgatggcgag caccacgtcc ttggcggtga cgccgcggcc gagctggccg tcgacgcgga
     1201 tcagcagggt cctggacttc ttctgcagca ggcattgggt ggcgagcacg tgctcgacct
     1261 cggaggtgcc gatgccgtgc gcaaggcagc cgaaggcgcc gtgcgtggag gtgtgggagt
     1321 cgccgcagac gacggtcatg cccggcagcg tggcgccgtt ctccggcccg atcacgtgca
     1381 cgatgccctg gcgcgcgtcc ttgaacggga aataggcgag cgagccgacc tcgcggatgt
     1441 tggcgtccag cgtctcgacc tgctggcgcg acaccgggtc ctggatgccc aggtcccagt
     1501 ggtcggtggg cgtgttgtgg tcggcggtgg cgacgatgga gccgatgcgc cagggcttgc
     1561 gcccggcgag cttgaggcct tcgaaggcct gcgggctggt cacttcgtgc accaggtggc
     1621 gatcgatgta gatcagcgcc gtgccgtcgg cctcctcgtg gaccacgtgg ctggaccaga
     1681 gcttttcata cagcgtttga gctttcatcc cggagtgttc cgcgtctgaa aaaggaatcg
     1741 attatttcac aaagcctgca aaactgtgta ggcacgcccc gccctcagcg ccgcgcgccc
     1801 tcgtagagcg ggatcacgcg tgcggcgtac tctgccaggt cgcgctgccg cgtcgcatgc
     1861 gaggggtggg tggacagcca ctgcggcggc gcgccggccg actgtccggc catcttgttc
     1921 cacagcgtca ccgcggcacg cggatcgtag ccggcgcgtg cggcgagttc cacgcccatg
     1981 cggtccgcct cggtctcgtg ctcgcgcgag ttgggcagct cgaaggtcac cttggcaacc
     2041 tggcccatga ggtcctggcc gagctggccg acaccgagca gggcgcccgc gaccgagatg
     2101 cccagcccgg tcgccatcga cttcgacacc cgctcgcgcg cgtgctcacg cagcgcatgc
     2161 gcgatctcgt ggcccatcac cgcggcgatc tcgtcgtcgg tgagcgcgag gcgctcgatc
     2221 agccccgtgt agatcgccat cttgccgccg gccatgcacc aggcgttgag ctcgctcgag
     2281 ctgaacacat gcacctccca ggaccacgac ggggcatcgg tgcggaaggc gccggtctgc
     2341 gcggcgagcc gcgagacgat gcggcgcacg cgctgcacgg tggcggggtc gcgatcgagc
     2401 gcgttcttgc tccgcgcctc ggcgaccatc tgctggtact gctggttcga ggcctgttcg
     2461 acctcctgcg ccgagaccat catcagctgc gagcgctgca cgccgaccgc gccgccgtgc
     2521 gtggtctgca cggtctggca ggcggtggcg atggtgccgg cgagcagcac cggcagggcg
     2581 atcttgaaga tggattgcat gggtcgatta cctcctcggt tcgtggagtg cgtgcgcggg
     2641 gcggggttcc cctttccagc ccagcagcaa ccagagcagg ccggcgagcg cgaagccgga
     2701 gccgatcgta tacgtccacg ccggcccgat gccctcccag gtgtaaccgc tcagcaagcc
     2761 gcccagcatg ccgccggccc cgaaggagat gctgccgtag agcgcctggc cgcgcgactg
     2821 caggcggccc ggaaaccact cgttgaccac cgcgatcgcc gcggcgtggt aggcgccgaa
     2881 ggtggcgccg tgcagaacct gggcgagcag cagcagcgcc aggctgtgcg cgccccagcc
     2941 gatcatcagg aagcgcgcca ccgcgcaggc gaaggagaac agcaggatcg cacgcagcga
     3001 aaagcgcacc gagatgcgcg gcatccacat gaagaccccg atctcggcga gcacccccag
     3061 cgtccacatc cagcccacca cgcccttgtc gtaacccatg ccgaccaggt ggatcgaata
     3121 gaacacgtag agcgcgccgt gtgccgccga catcaggaag caggcgccga gcagggcacg
     3181 cacctcggga cggcgcaggg tgtcgccgaa gctcgcggcg tcgcgctgca gcggcgggcg
     3241 cggcgcctcg gggacgatga agctgcacag cacgatgccg acgaggatgg acatggtgat
     3301 ccacagcacc gcctcgatcg gcaggcggtc gagcgaatgc ccgagcgcga gcacggcgac
     3361 gatgaagccg accgagcccc acacgcggat gcggccatag cggtgcgaag cctcgccgag
     3421 gtgggcgaag gcgagaccct cgatcagcgg cagcaccgcg ctccagaaga aggccatcag
     3481 cgccatcgcg gcgaacagcc cggcgaactc ggtggtcagg aagaacaccg agaagcccgc
     3541 caggctggcg agcgccgaca gacgcacgat cgcgatgcgc atccccaggc gttccgccag
     3601 ccagccccac aggttgggag ccagcacgcg catcagctgc atcagcgaca tcagcagcgc
     3661 gatgtcggtg gccgacagcg cgatcgactg caggtagagc gtgaagtagg gcgagaacgc
     3721 gccgacgaag gcgaagtaga agaagtagta cgccgacagg cgccagtagg gaatcatcgc
     3781 gcccactccg ggtacgcgcg ctcaccccct ccagcccctg cgtccgggcg gcgccccgca
     3841 ccgccccgcc ctgcccgcat cgcctcagcg cggcgaaggc aggcgctggc tccctgcggg
     3901 cgcgagcgcc gggatgcgcg gggtgtcggt gcgcacgtcg ccgcactgcg cgcgatggcg
     3961 cagcgcgtgg tcgatcagca ccagtgcgag catggattcg gcgatcggcg tcgcgcggat
     4021 gcccacgcag gggtcgtggc gaccctcggt ggcgaccatg acgggattgc ctgcgcggtc
     4081 gatcgagcga cgctcgaggc ggatgctcga ggtcggcttg atcgcgatgc tggcgagaat
     4141 gtcctgtccg gtggagatgc caccgagcac gccgcccgca tgattggaca ggaagcccgc
     4201 gggcgtcatc tcgtcgccgt gctcggtgcc gcgctgcgcg accgacttga acccggcgcc
     4261 gatctccacg cccttgaccg cgttgatgcc catcatcgca taggcgatgt cggcatcgag
     4321 ccgcccgtac accggctcgc cccagccgac cgggacgccg ctggcgacca cgtcgatgcg
     4381 cgcgccgatc gagtcgcccg acttgcgcag cgcgtccatg taggcctcga gctcgggcac
     4441 gatcgcggcg ttgggcgcga agaaggggtt gccgtcgacc tcgtcccagg acacgaaggg
     4501 aatctcgatc gggccgagcg cacccatgca ggcgcggatg acgatgccgt ggcgctcctt
     4561 cagccacttc ttcgcgatcg cgccggccgc cacccgcacc gcggtctcgc gcgccgacga
     4621 gcgcccgccg ccacgatggt cgcgcaggcc gtacttctgc aggtaggcgt agtcggcgtg
     4681 gccggggcgg aaggtgtcgg cgatgttgcc gtagtccttg ctgcgctggt cctggttgcg
     4741 gatcagcagc gagatcgggg tgccggtggt gacgccctcg aacacgccgg agaggatctc
     4801 gacggtgtcg ggctcgcggc gctgggtgac gtggcgcgag gtgcccggct tgcggcggtc
     4861 gagctcggcc tggatgtctg cctcgcagat cgccagtccc ggcgggcagc catcgacgat
     4921 gcagccgatc gccgggccgt gggattcccc gaaggaggtg acggtgaaga gcgtgccgag
     4981 ggtgttgccg gacatggatt tcctgcctga tcgcgatgac ggaacgggga cgggtcgcga
     5041 gtctagcacg cgtgccgtcc ccttctgctt gggcgccgga gcgccgcccc tcagtggaag
     5101 aagccctcca ggcgctcgaa gacctcgtgc tcggcgccat gccggcgcac attgagcacg
     5161 tcctcgagct tctcgagctg gcgcaccatc tgctcgaggc gctggtcctc gaacaccagc
     5221 agccagatgc gcgagcgctt gccgtcggac accggcatgc acaggatgcc ctcgacgttg
     5281 aacgcgcgac gcgcgaagag gttgcagatg tggctcatca ccccggggtg gttgttcaca
     5341 tccagttcca gaacgacctt ggcatagccg gcttgcggca gcgggtcgga aacctgttcg
     5401 atcatcatca gcctccgatc atctcggtgt tggcggcacc cggcggcacc atggggtaga
     5461 cgaattgttc gcggtcgatc gtcgcgtgga tcaggcacgg accggggcgg tgcagcgcct
     5521 cggcgagcgc ggcgcgcgga ttgtcggcga gatcgagatc caccgccggc acgccgaagc
     5581 cctcggcgat cttcacgaag tcgggctcgg tgcggtactt cgaagcgaac aggcgctgac
     5641 cgtagaacag cgtctgctgc tggtacacca ggccgagcgc gttgttgttc atcagcacga
     5701 tcttgacgtt cagaccctcc tcggccagcg tcgcaagctc ctggatgttc atcttgaagc
     5761 tgccgtcgcc ggtgaagcag accacggtgc gcccgggctc ggcgagcgcg gcgccgagcg
     5821 cgctcggcag gccgaagccc atcgtgccca ggccgcccga ggtcagccac tggcgcggcc
     5881 ggcggaaggg ataggcctgc gccacccaca tctgatgctg gccgacgtcg gtggcgacga
     5941 tggcgcggtc gtcgagcgcg gcggcgaccg cctggatgag gccgtagtgg gtgcgcgggt
     6001 cgtcctggcc ggggaactgc atcgggaagc gcgtcttcag gctgtcgaca tgcgacagcc
     6061 agcgcttgcg cagcgtcgcc tgcacgcgcg gcagcagcgc ctcgagcgcg cacttgacgt
     6121 tggcgtggat ggcgacgtgg gcgttcttga tcttgtgcag ctcggagcgg tcgatgtcga
     6181 tgtggacgat cttcgcgttc gggcagaact gcgcggcgcg gccgatcgcg cggtcgtcga
     6241 agcgtgcgcc cacgcacacc agcacatcgg cctcctcgag cacgaagttg gtgtagcgcg
     6301 cgccgtgcat gccgagcatg ccgatcgaca gcgggtggtc catcggcatc gcaccgagcg
     6361 ccatcagcgt catcgtggtg ggcaggccgg cctgctcggc cagctgcacc gccagcggcg
     6421 cggcgccgga ggcgaccacc ccgccgccga ggtagagcac cggctgctcg gcctcgttga
     6481 tcatcttcgc cgccagctcc accgccgcca ggtcgagctg cggggccgga tcggcgaccg
     6541 ccggggcggg aaactccgcg accgcgacga gctggttctg cacgtccttg ggcacgtcga
     6601 tcagcaccgg gccgggacgg ccggacatgg cgatgcggaa ggcgtccgga atgacctcga
     6661 gcagctcctg cgccgagcgc acgaggaagt tgtgcttggt gatcggcacc gtgatgccat
     6721 agatgtcgac ctcctggaag gcgtcggtgc cgatcatcgc ctgcggcacc tgcccggtga
     6781 tgacgaccat cgggatggaa tccaggcagg cgtcggcgat cgcggtgacc aggttggtcg
     6841 cgcccggacc gctcgaggcg atgcacacct gcggcacgcc cgagacgcgc gccatgccct
     6901 gggcgatgaa gcccgcgccc tgctcgtggc gggcgagcac gtggtggatg tgctcgctgc
     6961 ccgagagcgc atcgtagaag ggcaggatcg cgccgccggg aatgccggcg acggtgcgca
     7021 cgccctggcg ctcgagaagg cgcaccatca gttgcgcgcc ggtcatttgc atcatgttgc
     7081 tctccttggc ttcgggttcg acggccggac cggcgggcac aaaagcaaga cccccgccgg
     7141 gcttgcgcgc cgacgggggt ctggtattcc tgattctcga gtgtccggtc ccgttacgtc
     7201 gcgcgcatgc ctacgcctac tacgcgtact acaacgacca gcgacaggag cgagggacgg
     7261 ccggaaacgg acattggaac ggatctgcaa gaaagcgtaa acaacgtgac ggcgagttta
     7321 atcacgaaaa gatgcactgc acaagaaaat tttcgcgtgc cggaccgcgc gcggggaggt
     7381 gcctgcgccc tccccgcgcg cgctcacttc ggccgcagct cgcggcgcag gatcttgccg
     7441 acgttgctct tgggcagttc gtcgcggaac tcgacgtggt gcggcacctt gtagccggtg
     7501 aggttctcgc ggcagaaggc gatcagggcc tcggccgtca gcttcggatc cttgcgcacc
     7561 acgaagacct tcaccgcctc gccgctgcgc tcgtcgggta cgccgatcgc ggcgacctcg
     7621 agcacgcccg gatgagcggc gaccacgtcc tcgacctcgt tcgggtacac gttgaagccg
     7681 gacaccagga tcatgtcctt cttgcgatcg accaggcgca cgaagccgcg ctcgtcgacg
     7741 gtggcgatgt cgcccgtgcg caggaaaccg tcgtgcgtga agattcgcgc ggtctcctcg
     7801 gggcgctgcc agtagcccgg cgtgacctgc ggaccgcgca cgcagagctc gccgcgctgg
     7861 ccgagcggca cctcatgccc ggcatcgtcg cggatgctga tctcggtgga ggacaccggc
     7921 aggccgatcg aatggttgaa ctcggcgaga tcgagcgggt tgatcgtcac cgccggcgag
     7981 gtctcggtga ggccgtaggc ctcgaccaga gacaccccgg tgaccttctt ccacttctcg
     8041 gcgaccacct gctgcaccgc catgccgccg cccagactca cccgcagcgt ggagaagtcc
     8101 agcttggcga actcggggtt gttgagcagt gcgttgaaca gcgtgttgac cccggtgatc
     8161 gcggtgaagc ggtgcaggcc gagctccttg atgaagcccg ggatgtcgcg cgggttgggg
     8221 atcagcacgt tcttggcgcc gatcttcagg aaggtgaggc agttcgcggt gagcgagaag
     8281 atgtggtaga gcggcagcgc ggtgatgatg atttccttgc cctcgcccac gtagggggcg
     8341 atccacgcat gcgcctgctg caggttggcg atgacgttgc gatgagtgag catggccccc
     8401 ttggccactc cggtcgtgcc gccggtgtat tgcaggaagg cgacatcctc atggcccacc
     8461 tgcgccggct gcagctcgtg ggcagcgccg cgctgcaagg ccgtggaaaa gcgcacgtgg
     8521 ccgtcgagcc gccacggcgg caccagcttg cgcaccttgc gcacgacgaa gttcacgatc
     8581 gcgcccttga ccaggccgag catctcgccc aggctggtga ccacgatgtg gcgtatcggc
     8641 acctgcgccc gcacatgctc gagcgtgtgg gcgaagttct ccaggatcac gatgacctcg
     8701 gcgccggagt ccttcaactg gtgctcgagc tcgcgcgcgg tgtagagcgg attgacgttg
     8761 accaccgtgt agcccgcgcg cagcgtgccg aagagcgcga tcgggtactg cagcacgttg
     8821 ggcatcatca gcgcgacgcg cgcaccgcgg ggcagcttca gcgcgccctg cagccaggcg
     8881 ccgaagcgcg cactgagcgc gtcgagctcc gcgtaggtga tcgacttgcc catgcagtga
     8941 taggcggtct tgtccgcata gcgcgccacg ctgcgctcga acaggtcacc cagggaacgg
     9001 aattcgtcga cgtcgatttc cgccggaatg cccggggggt agctcttcag ccagattttt
     9061 tccatggtct cctccttttg ctggaaatac cgccggagcg ccgcgatggc gccccggctc
     9121 cgccctccca aggcgtcgga cgggcaagcg gcccttccgt tcattccaac catgccgcac
     9181 ccgatcttgg tgtgggtgtc gaccgaacgg gcgcagcccg ttcctggagg tgcggcggtt
     9241 cgaccgccgc tgccgggcgc ggggcccggc gatgctgctt attcctcgcc ctcgcccgcg
     9301 ggccagttgc ggatgtagtt cttgagcatg cggttctcga agctctgctc ctcgagcacc
     9361 gccttggcga cgtcgtggaa actcaccaca ccgagcagcg tgttgccgtc catcaccggc
     9421 aggtagcgct ggtggtgctc gaccatcagc cggcgcagct cgtccatctc catgtcgggc
     9481 gaggccacgc gcggctcacg cagcatgacc gcctcgacct gcagcgactg ccagtccgcc
     9541 ccgccctgct gcaccgcctg caacacctgc cggaaggtga gcatgccgac catctcgcca
     9601 cgcgagaaca cgaccagcga gccgacgtcc tgctcgttca tgatctcgac cgcctcggcg
     9661 atgggtcggt ttgggccgat ggtgaataac accttgccct tgatcgcgag aatctcgctc
     9721 accagcatgt cggtctccgt gccgttgcac ttacaggttt gatgacgctc accatcttag
     9781 tgcatcgccg cgtgcgcgca aagccagccg cggcgtgcct tcagcgcttc agctcgccga
     9841 gcttgccctt gatgcgggcc cagtggtcgg catccggcag aggatccttg cgctcgacga
     9901 tcggcttcca ttgtttggcc agttcggcgt tgagcgcgat gtaacccttc tggtcgtccg
     9961 gcacgtcgtc ctcggaatag atcgcctcga ccggacactc ggccacgcac aaggtgcagt
    10021 cgatgcactc ctccggatcg atgacaagga agttgggccc ctcgcggaaa cagtcgaccg
    10081 gacagacgtc cacgcagtcg gtgtatttgc agcgtatgca gcgctcggta acgacgtagg
    10141 tcatctcgac tctccaggca ggatcggccg ccgcccgctg cggctggccg aaggggggac
    10201 aatatagacc agcccttggc ttttttgcca cggcaagcag gcgcttgccc gcccgccagc
    10261 agcgatttcc ttgacggcga ccccggcatc gcataagttt cggaaaaatt tctttacgga
    10321 aggcctctcc ttccgctgga tcgtcgtgca tcgcagcagc gcctcccgct cgcggcgaga
    10381 tcgcaggggg tccgcccctt cccgcacgca gcatccgttc gcattacccc gcaacacccc
    10441 ataccccgca gcacccgtta cccggcaacg agggcttccg ccttcgtcac caccccctgc
    10501 cagccttagt gaggaatcat gagcgagcac atccaccatg tgaccgacgg caacttcgac
    10561 gatgaagtgc tgcagtccca gacccctgtc ctggtcgatt actgggccga gtggtgcggc
    10621 ccctgcaaga tgatcgcgcc gatcctggac gacgtcgcca aggactacgc gggcaagctc
    10681 aaggtcgcca agctcaacat cgacgacaac caggagacgc ccgcgaagta cggcatccgc
    10741 ggcatcccga cgctgatgct gttcaagggc ggcaatgtcg aggcgaccaa agtgggcgcc
    10801 ctttccaaat ctcaactgac cgccttcatt gacagcaaca tctaagccgg accaggctcc
    10861 ggcccgttcc cgcggttcct cgcgcgcccg tcgccgcggc gcgcgcaacc gggacggcaa
    10921 ttccccccag aatccgcaga ccgaagacga tctgttcgac gtcgagaacg gcggcgagga
    10981 aggttcttcc acgccgcgcg accccgacgc gcccgcgctc cacctgtccg aactcaaggc
    11041 cctgcacgtc agccagctgc tgcagatggc gatggacgcc ggtgtcgagg gcgcgaacag
    11101 gctgcgcaag caggaactcg tcttcgcgct gctgcgcaac cgtgcccgca ggggcgaacc
    11161 gatctgtggc gacggcgcac tcgaagtgct gcctgacggc ttcggcttcc tgcgctctcc
    11221 cgacacctcg tatctcgcgg ggacggacga catctacgtg tcgccctcgc agatccggcg
    11281 cttcaacctg cgcaccggcg acaccatcga gggcgagatc cgcacgccca aggacggcga
    11341 gcgctacttc gcgttgacca agctcgacaa gatcaacggg cggccgccgg aagagtgcaa
    11401 gaccaagatc ctcttcgaga acctcacgcc cctgcatccg caggagtgcc tgaagctcga
    11461 gcgcgaggtc cgtggcgagg agaacgtcac cagccgcatc atcgacatga tcgcgccgat
    11521 cggcaagggc cagcgcggcc tgctcgtcgc cccgcccaag agcggcaaga cggtcatgct
    11581 gcagcacatc gcgcacgcga tcacggccaa ccatcccgac gtcaagctga tcgtgctgct
    11641 gatcgacgag cgcccggagg aagtgaccga gatgctgcgt tcggtgaagg gcgaggtcgt
    11701 ggcctccacc ttcgacgagc ccgccacccg tcacgtgcag gtcgccgaga tggtgatcga
    11761 gaaggccaag cgcctgaccg agcacaagta cgacgtggtg atcctgctcg actcgctgac
    11821 ccgtctggcg cgcgcctaca acaccgtggt gccggcctcc ggcaaggtgc tcaccggtgg
    11881 tgtcgacgcc aacgcgctgc agaagcccaa gcgcttcttc ggtgcggcgc gcaacatcga
    11941 ggaaggcggc tcgctgacca tcatcgccac cacgctggtc gacaccggca gccgcatgga
    12001 cgacgtgatc tacgaggaat tcaagggcac cggcaacatg gagctccacc tcgaccgccg
    12061 catggcggag aagcgcgtgt atccggcgat caacgtcaac cgctccggca cccgccgcga
    12121 ggagctcctg ctcaaggccg acgtgctgca gaaggtgtgg atcctgcgca agctgctgta
    12181 cgggatggac gacatcgacg cgatggaatt cctcctcgac aaggtcaagg ccaccaagag
    12241 caacgccgag ttcttcgacg cgatgcgcac cggccgcggc tgattccgcg ctcgacagca
    12301 aaacgccacc ttggaggtgg cgttttttct tgtgcggccg cgccgtgcgc ggccgacgct
    12361 cacttgtagt cgtagaagcc cttgccgctc ttgcgaccga ggtagcccgc ctcgagcatc
    12421 tcgaccagca gcggcgccgg gcggtacttg gggtccttgg tgccttcata gaggacctgc
    12481 atgaccgcga gctcgacgtc caggccgatg aggtcgcaca gcgcgagcgg gccgatgggg
    12541 tggttgcagc cgagcttcat cgcctcgtcg atctcggctg cggtcgccag gccctcgccg
    12601 agcgcgaaca ccgcctcgtt gatcatcggg cacagcatgc ggttgaccac gaagcccgga
    12661 ctgttcttga ccttgatcgg cgtcttgccg atcgccttcg cgacgtcctc gaccgcggag
    12721 taggtcgccg ccgaggtctg caggccgttg atcagctcga cgagcgccat caccggcacc
    12781 gggttgaaga agtgcatgcc gatcacctgg ccgggacgct gggtgccggc ggccaggcgg
    12841 gtgatggaga tcgacgaggt gttgctggcg agaatcgcct ccggcttcac gatacggtcg
    12901 aggtcggcga agatgcgcag cttgagatcg acattctcgg tcgccgcctc gatcaccagg
    12961 tcgcagtcgg cgagcgcatc gagcgagctc acggtggtga tgcaggccat cgcggcgtcc
    13021 ttctgcggcg cgctcatcct gtccttcttg atgaggcggt cgaggctggc cgagatggtc
    13081 gcgagcccgc gctgcacctg cgcctcggcg acgtccatca tcaccacctt gcgccccgcg
    13141 accgcgaacg cctgggtgat gccgttaccc atcgtgcccg cacccaccac tccgatcttg
    13201 ccgatactca tcgccttcgt tctccgcaca ggattttggt cgacgcaacg gccgccggac
    13261 gacggtctcc ataccgctgc gtatgcgcgg ggactttagc ggattccgcg ggcgggtgcc
    13321 agcgtcgcgc ggccgcacag cgggaggatc gaaaggcgct ccacccaagc gtttctcgat
    13381 gctcggcagc tcgggcgaag cgctgcaagc aagccggctt ttcgatcatt tctcgatatt
    13441 tttcttgtga gtcccccggg gcagcggcta cattagagag gcctccatcc gtcccccttc
    13501 ctctccaaga tcatgctcca cacctcgcct ccctacgccg tccgccaaga aaccgaggtc
    13561 gccgaaccga agacgctggt cgaggcggcc tatcgcagcc tgcgccgcaa catcatcgag
    13621 ggccgcctgt cgccgggcga gaagctgcgc atcgagcaca tcaagaacga ctacggcgtg
    13681 gggggcgcga cgctgcgcga ggccctctcg ctgctcacct cggatgcgct cgtggtgtcg
    13741 cagggccagc gcggcttccg cgtcgccccg gtctcgctcg aggacctcgc cgatatcacc
    13801 gagacgcggg tgatgctcga gtgcagcgcg ttgcgccagt cgatcacgct cggcgacgag
    13861 gcctgggagg ccgagctcac cgccgccttc caccgcctca cgcgcgcgga ggagaagctc
    13921 gccgaaggca acaatcgcga gcagtgggaa gagcgcaacc gcatcttcca cgaggtgctg
    13981 atcgccgcct gcccctcgcg ctggaccaag cacttcctgt ccatcctcta tcaccaggcc
    14041 gagcgctacc gccacctctc gctgcgtcgc agcgacggta cgcgccggct gcacgacgag
    14101 cacgccgccc tgttcgaggc cgcgatgggg cgtgacgccg agactgccac ccgcctgctc
    14161 ggcgaacaca tccgcctgac gctgcagtcg gtcagcctcg acaaggccgc caccgccagc
    14221 cactgagccg gccacccggg cttgccgccg cacagcggcg aacagtcaaa aaaatggccc
    14281 gccgaagcgg gccaaacccg aaatcacctc tgcgcgggcg cgcagaaggc aaccacgacg
    14341 aactgctcga tttcggggag ggagagcctg ggatggagag ggccgcgggg cgcgcgaagc
    14401 ctgcggccat ctccgccgga gcgtgtgctc aaccgccgcc cagcccgacc ggcgccgggg
    14461 cggtgttgac gatgcgggtg aacagcgccg cgtacttgcc cgccggtccc tgccaggggt
    14521 tgtcgttggc gaggaaggcg gcgtcgatgt gcgcccagtc ctccgcgctc aggtgcttcc
    14581 gggcgagcgg gaagatcagg tgctcctcgg tgttcatgtg ctgccactcg tgctcgatgt
    14641 aggtcttgag cgcgccctgg aagcccgcca ggccggattc gccttcgcgc cggtaggcct
    14701 cgagcgccgc acgcaaggtg gccatgcgcg catcgccctc gcggtgctcg gcctccaggc
    14761 gctcgatgat cggcagcgcc tccgcgctgc gctcgcgcag gcgggcgaag aggtagctgt
    14821 cctccttggg gtggtgcacc ttctccggga ccatctcgat gtactcgatc atcgacgcca
    14881 tcagctcgaa atcgggcttc agccgccccg cctcgatcga ggcgacgagc ccgcgcaacc
    14941 cgctcagcat cgcggcgagg gcgcggtgct cgtcatggat gatgtccagc gacttgttgc
    15001 tcatgcttgg tttcctcctt gctctcagac gccgagatgc ttctggatca gctccggctg
    15061 ggcgcggatc tcgtcgctgg tgccgtcgaa tacgacctgc cccttgacca ggatcatcgc
    15121 gcggtcgcag atgctggtga ccgcgccgtg gttcttgtcc acgatcacgg tggcgatgcc
    15181 ggtcttgcgg atctccttga cgatgcgcca gatctccatc gcgatcagcg gcgcgaggcc
    15241 ctcggtggcc tcgtcgagga tcagcagatc ggggttggtc atcagcgcac ggccgatggt
    15301 gagcatctgc tgctcgccgc ccgagagctg ccagccgccg ttgttgatgc gttcgcccag
    15361 gcgcgggaag gtctccatca cgcggtcgaa ggtccactcg cggcgaccat cgacgccggc
    15421 gcgcgccgac atcagcaggt tctcgcgcac gctcaggctc ttgaagatgc cgcgcccttc
    15481 cggcacgtag gcgatgccct gctgcgcggt ctggaacgtg cgcgccctgg tgtagtcgtc
    15541 gccgcgcacg agcacccgac cgtgcctggg gcggacgatc ccgagcatgc tcttgatcag
    15601 cgtggacttg cccatgccgt tacggcccat gagggcgatg ccctcgcctt cgcggatgtt
    15661 gaagtcgatg ccgtgcagga tgtgactcgc accgtagtag gtgtggatct ccttggcttc
    15721 gagcagcatg ttagacatgg aagctgtcct ccgtacccag ataggcctgc tggacggcca
    15781 cgctggcctt gatctgcgcg ggcggcccgc actccagcaa ctggccgttg accatcaccg
    15841 tgatcatgtc ggcgaccgcg aacaccgcat ccatgtcgtg ctccacgagc aggatgccgc
    15901 gcgtctggcg caggcgcttg agcacctcga ccatgctcgc cgactcctcc gaacccatgc
    15961 cggcgagcgg ctcgtcgagc agcagcacct gcgcgtcggt ggccagcacc atggcgatct
    16021 cgagctggcg ctgctcgccg tggctgagca cgcccgacac ccagtcgacg cgcttgccca
    16081 ggccggcctc ctcgatgcag gcgcgcgcct tctccagcgc ccacttctgc tccatcgcct
    16141 tgctgaagag gcgccacggt tgctggcggc gcgactgcgc ggccaggcgc acgttctcga
    16201 gcacggtgaa ctgcgggaag atgttggtgc gctggtagct gcgcccgatg cccacctgcg
    16261 agcgctgcgc cgcggtaagc gcggtgatgt ccctgccgag gtagtggatg cgcccctcgg
    16321 acggcggcag gtcgcccgaa agcatgttga tgcaggtcga cttgccggca ccattggggc
    16381 cgagcagcac atggatcatc tggcgctcga gcgtcacgct gatgtcctgg ttcgccacca
    16441 g
//
LOCUS       DAOQBS010000063        16310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2647_length_16310_cov_16.859120, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000063 DAOQBS010000000
VERSION     DAOQBS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 16310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16310
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2647_length_16310_cov_16.859120"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..1265)
                     /locus_tag="PKN25_08750"
     CDS             complement(291..1265)
                     /locus_tag="PKN25_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNV91028.1"
                     /translation="MNLNPQQARSLVAICLHAAFADGHQGAVERDHVRKVAESLGREA
                     EIDFIALYQDVLLGGLGLEAAVAGLGEANLRQLAFELAVGVCEADGGLDAREKDFLER
                     LSGLLELSRPAADAFVREAEALGQAPADGPLASLPGLGEDEPAAAPSAAAAPDAAALD
                     RTILNASILNGALELLPQSMASMAIIPLQLRLVYRIGQAHGYELDRGHIKDFLAAAGV
                     GLTGQYVEQFGRRLIGGLLGKVLGGAGRAVGSQATGSAFSFATTWAIGKVAVQYYGGG
                     RTVDGDALRQAFAGLFEQAKAMQERYAPEIAQRARTLDLKRLTDELRR"
     gene            1464..2111
                     /gene="can"
                     /locus_tag="PKN25_08755"
     CDS             1464..2111
                     /gene="can"
                     /locus_tag="PKN25_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNV91029.1"
                     /translation="MSQSIEHLFANNKNWSERMHSEDPEFFSRLVKQQSPEYLWIGCS
                     DSRVPANQVIGLAPGEVFVHRNIANVVVHTDLNALSVIHYAVEILRVKHILVVGHYGC
                     GGVKAAMNDNKTGLTDNWLRHIQDVRDRHIDRLERLTDPAERADRLCELNAMHQVVNV
                     CQTSVLREAWQRGQGVTVHGWCYSLQDGLVRDLGVSAGTREEAVERYRDAVERIG"
     gene            2128..3033
                     /locus_tag="PKN25_08760"
     CDS             2128..3033
                     /locus_tag="PKN25_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNV91030.1"
                     /translation="MGTRIGIDLGGTKIELVVLDADGRERWRRRVPTPQGDYGGTLRA
                     IAALVEEAERLTGAGARIGVGTPGSPSPRDGRIRNANSTCLNGQPLQQDLEALLRRPL
                     RLANDANCLAMSEAADGAGAGARTVFAAILGTGVGGGIVVDQKLLVGANAVAGEWGHN
                     PLPLPAPDDLPLPACYCGRAGCIETYLSGPGLAADHLRHGGEPLDAAAIARQATEGQR
                     ASQASLERYELRLARALAGVINLLDPEVIVLGGGLSQIARLYEHVPRRWAAHVFSDTV
                     STRLLPARHGDASGVRGAAWLWPSN"
     gene            complement(3301..5475)
                     /locus_tag="PKN25_08765"
     CDS             complement(3301..5475)
                     /locus_tag="PKN25_08765"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNV91031.1"
                     /translation="MAIVRVALPIPLPQAFDYTAADAGVGDVGRCVKVPFGRGERTGL
                     IVGIGDEAGVELARIKPVLHIQRGVPALPADWLELVSFVARYYHAPLGEVVALALPPG
                     LRRADGVSDEDEDPLLGVAPKGGEALCAARRASRALALLQALAAEPGPWRRSVIRARE
                     GGEAVGELLRRGWLEIVADPGARHEAGAQPLATPEQAAAVDAIVGAGMRFRPWLLHGV
                     TGSGKTEVYLRLAAHALAQGRQVLMLVPEIALTPQLEQRVAQRFAAANVVSLHSALAD
                     GARSRGFVQALSGRADIVLGTRLAVFAPLPRLGLILVDEEHDASYKQQEGVRYSARDV
                     AVWRGRQRDVPVVLGSATPSLESWFHARSERYALQRLSRRAVAATLPAVRCIDTRRTK
                     LDEGLSPALHVAITQRLERGEQSLVFLNRRGYAPVLSCPSCGWVSRCPHCSANLVVHL
                     ADRRLRCHHCGCDGPIPHACPSCGDQDIQPFGRGTQRIEARLAELFPQARVLRVDRDA
                     ARTRSQWETLLDTIAAGEADILVGTQMMAKGHDFPKLTLVGVVGADASLHAADFRAPE
                     RLFQQLMQVGGRAGRGALPGEVLIQTEYPMHPLYLHLAKHDFDAFARMALQERRAAGF
                     PPFTHQAMLRADAPALEDAVEFLRHARRLAEERAPAGLRLYDPVPMRMTRLARRERAQ
                     LLVEADHRGPLQAFLAEWVAVLYRQRTARELRWQLDVDPLEV"
     gene            complement(5867..6937)
                     /gene="hemE"
                     /locus_tag="PKN25_08770"
     CDS             complement(5867..6937)
                     /gene="hemE"
                     /locus_tag="PKN25_08770"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNV91032.1"
                     /translation="MSRLKNDTFLRALLRQPTEYTPVWLMRQAGRYLPEYCETRKRAG
                     SFLQLCKSPAMACEVTLQPLARYDLDAAILFSDILTVPDAMGLGLYFAEGEGPRFERP
                     LKDEWEIRNLSAPDPHAELQYVMDAVAEIRRALDGSVPLIGFSGSPWTLACYMVEGGS
                     SDDYRKVKSLAYSRPDLMHHILDVTAQAVVKYLNAQIEAGAQAVMVFDSWGGVLSEAA
                     YKEFSLPYLEQVVAGLIRERDGQRVPSIVFTKGGGLWLESIAAIGCDAVGLDWTMDIG
                     RARRLVGDKVALQGNLDPNVLFAPPEAVATETRRVLDAFGNHPGHVFNLGHGISQYTP
                     PESVSVLVDTVHAHSRAIRAGA"
     gene            complement(7038..8513)
                     /locus_tag="PKN25_08775"
     CDS             complement(7038..8513)
                     /locus_tag="PKN25_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNV91033.1"
                     /translation="MSSDARARAYYPALNALGLIIMIFGLLMGFPLVVSAALGDGATL
                     AYDQGLFVTFVAGLLLWAATRSKRRDLRVHDGFLLVAATWVVLPLFGALPLLAYIPDL
                     GFTDAYFEAVSGLTATGATVLTGLEHLPISINLWRTFMHWVGGMGVIVLVVAILPLLG
                     IGGRQLYKAEVPTPMKDSSLTPRIAETAKGLWLTYVLLTIACGFSLWWAGLAPWDAVI
                     HAFSVAGLGGFSNRDASLGHFDSVGVELVTAGFALLAGLNYATHYLALVRRSSAPYRR
                     DPEIPFYFGVLALSTLMLTAYLLAQGIFTDFGEALRYVAFHVVSISTSLGLATHDYTL
                     WPMFAQIWILFLGSFVACSGSTGGGIKMMRAMILYKQVIREIVRSLHPNAVRPVRMGR
                     NPVPEHILHAVLGFSFMYMVSIVSLTLVLSAAGLELVTAFSAVVACLNNTGPGLGLVG
                     PASNYQGLDDLHKWVLCFAMILGRLEIFTFLVVLTPTFWRR"
     gene            complement(8515..9918)
                     /gene="trkA"
                     /locus_tag="PKN25_08780"
     CDS             complement(8515..9918)
                     /gene="trkA"
                     /locus_tag="PKN25_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNV91034.1"
                     /translation="MKIIILGAGQVGASVAENLVSEANDITLVDTSEEYLGALRERLE
                     IRTVCGNGASPRVLREAGADDADLLIAVTQSDQTNLCACRIAKALFNLPTRIARLRST
                     DYVDHPELLDDDNFAVDFSICPEQVVTDYIKRLIAFPEALQVLEFADGVVSLIGVRAF
                     EGGPLVGHPLKALRFHLPNVEVRIAAIYRNGEPIMPEGDTIILPGDEVFCLAATVHIR
                     QVMRELRRSDRPMRRIMIAGGGNIGFRLAQSIEENHHVKILELNRTRAEMLASHLSNA
                     LVLRGDSTDEKLLETEGIDETDLFVALTNDEEDNIMSASLAKRMGARRTLALINRKSY
                     VDLVQGGPIDIAISPAHTSIGTLLAHVRRGDVVAVHSLRRGAAEALEIIAHGSSKDSK
                     VVGRPIEQIALPRGASIGAVVRDEKNEEGRVVRREVIIPHHDTVIREDDHVIVFCTNK
                     KLVQKVEKLFQVGFHFL"
     gene            complement(10049..11296)
                     /locus_tag="PKN25_08785"
     CDS             complement(10049..11296)
                     /locus_tag="PKN25_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV91035.1"
                     /translation="MAKILIVDDEIGIRELLSEILRDEGHDILLAENATAARAARNAV
                     RPDMVLLDIWMPDTDGITLLKEWAANGQLNMPVVMMSGHGTIDTAVEATRIGAIDYLE
                     KPIALQKLLAAVKRGLQRPQAPGVQSAPTLAAFHRSVPLRELRRRLEQIAGSSRVLLL
                     RVGAGSLAELVARSVQPANAPWLDLAAVTAPLDITQLQSTQGGVVFVGELARLSRAQQ
                     KNLAFALDRLERYDLRLVVATDRSLEALVGEGWEEGLLARLFEVSLAPPSIADVREDL
                     PELASQMLLHLVEAGEVPLRRFSTAALNQLRNLPWPGGYPELRAAVKSLALGTLEEEI
                     GAGEVRRLLPPDLGAGACGVSLDQPLREAREAFERMYFEHHLRLEGGNMTRLAEKTGL
                     ERTHLYRKLKQLGLQAGRRHEES"
     gene            complement(11306..13423)
                     /locus_tag="PKN25_08790"
     CDS             complement(11306..13423)
                     /locus_tag="PKN25_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV91036.1"
                     /translation="MKRVLLAVAAALSGISLFLLTSASSNTTLFATSYPWLLALNGGM
                     AVVLAALVGMQLHRLLREHREGQFGSRLKMRLVLMFALMGVVPGLVIYGVSLQFVVRS
                     IESWFDVRVDSALEGGIALGQNALDYLVSQVRDKADDMVLELEGAGQGAGTRLNRLRE
                     QAGIASATILAPAGQVLATAAESGRTLLPELPGTAELRQARQSRHFHSVIAEPDGRLV
                     IRVLTPIPVRTLAGESNLLMLQQPVPESFGRNAEAVQEAYREYQQLTLGRSGLKRIYT
                     VTLSLTLLLALLGALAVAVLIARRLAQPLLILAEGTQAVAQGDFSPRQALPANDELGV
                     LTQSFNRMTRQLQEARAAADRNRAEVEAARAYLESVLANLSTGVLAFSADGHLRAANH
                     GALQILEDDLGGFEDIPLEAWPRQAELGHMLREGFTANTGDWQKQMELPVRDRPPRTL
                     LIRGSRLPESTGGGLVVVFDDISSLIAAQRTAAWGEVARRLAHEIKNPLTPIQLSAER
                     LSFKLADRLDDNGREMLERATTTIVNQVEAMKNLVNAFRDYARLPAPQLAPLDLNALI
                     REVLHLYESAAVQIRTELGGALPPVQGDPTQIRQVIHNMLQNAQDALGAQEDAEISVI
                     TRRDGESALLLCRDNGPGFPPEVLARAFEPYFTTKSRGTGLGLAMVKKIVDEHGGEVR
                     LSNREGGGAEVRIRLRLAENKEA"
     gene            complement(13420..13959)
                     /locus_tag="PKN25_08795"
     CDS             complement(13420..13959)
                     /locus_tag="PKN25_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HNV91037.1"
                     /translation="MAALLLLWALSVRAESVIRSAEIVPGDGGYVLNADIAIDLNPRL
                     IDVVTRGVSLYFTTEVLIERPRWYWLNETVARRSLDYRLYYHAITRSFRLSVGNLHQN
                     FDELDEALRTMQRVRNWLVAETGVLQSGVSHEVSLRFRLDTSQLPKPFQVTAIGSNDW
                     NVETEWVRWTFLPGAPGFR"
     gene            complement(13979..15286)
                     /gene="rsmB"
                     /locus_tag="PKN25_08800"
     CDS             complement(13979..15286)
                     /gene="rsmB"
                     /locus_tag="PKN25_08800"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNV91038.1"
                     /translation="MRGAPRRAPEQPIDSLGYALARATELVAGVIDGANLTDAFERMQ
                     AGHPEWPEGTRGAVRDLAWSTLREFGRGDAILSRLLHSPPPVEIRALLLVALQRLTQR
                     PEQAHTVVDQAVGATAVAMPGLRNLVNGVLRNALRRQPEWQGWIEAEPEARHAFPAWW
                     VERVRSAHPQAWQDLLAAGNTRPPMALRVNPRRATLAEVEAELAAAGLEFRRLDNDAL
                     VLARPLAVARLPGYAEGRLSVQDAGAQWAAQLLDARAGERVLDACAAPGGKTAHILER
                     ADADLLALELDPLRAGRVARNLDRLGLRAELKVADCRRLAAWWDGRPFDRILADVPCS
                     ASGVVRRHPDIKWLRRDSDIANFAAQQAEILEALWRTLAPGGTMLYVTCSVFDEENAG
                     QVARFCVRHADAERLPIRGSSDLQLLPCADHDGFYYALLRKRP"
     gene            complement(15395..16285)
                     /gene="speE"
                     /locus_tag="PKN25_08805"
     CDS             complement(15395..16285)
                     /gene="speE"
                     /locus_tag="PKN25_08805"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNV91039.1"
                     /translation="MSGASWFADLPKDVLAELLGEDAGYFLRGARLLESGRSEWQDYE
                     VWETPRFGKLFRLDGCFMTSERDEFHYHENLIHVPGVAHPGLRRALLIGGGDGGSAEE
                     LLKYPGIERIVIAELDAKVVDIARRYLDAVHHGALDDPRVEIHIGDGMAHVCEHAPAT
                     GERYDLIVLDLTDPIGPAEALYTADFFAACKALLAEGGALTLHIGTPVFQPERVRELL
                     ARLRRVFARVRPYFLYIPLYGSLWGMACASDTLDPAALDANEVDRRLAERAIRELQHF
                     NGAVHCAQFALPNHLRALLA"
BASE COUNT         2688 a         5612 c         5663 g         2347 t
ORIGIN      
        1 ctcgacaccg ttggacaagg tgagcagggg agctggcgtg attttcatga aaatctcgag
       61 aaagagagag ggactacagg attccacatt ccggcgtctt atgtaaggac cctgggaacc
      121 acgccagtgc agatccgtcg ggcgtggcgc cgtcacaggg tcagcgcgca ccgaggcatg
      181 gcctcgaaac gggcaggccg gcggggcggc ctccgcgtgc catctcccgc cttgcaatca
      241 ctcccgaggg cggttccacg tgaaaccgcc ctcgcgcggc cctcccctgc tcagcgccgc
      301 aattcatccg tgagccgctt cagatccagc gtgcgcgcac gctgcgcgat ctcgggcgcg
      361 tagcgctcct gcatcgcctt ggcctgctcg aacaggcctg cgaaggcctg gcgcagtgca
      421 tcgccatcga ccgtgcggcc gccaccgtag tactgcaccg cgaccttgcc gatcgcccag
      481 gtggtcgcga aggagaaggc cgagccggtc gcctggctgc ccaccgcgcg ccccgccccg
      541 ccgagcacct tgccgagcag gccgccgatc agccggcggc cgaactgctc gacgtactga
      601 ccggtcaggc ccacgcccgc tgcggcgagg aagtccttga tatgcccgcg gtcgagctcg
      661 tacccatgcg cctggccgat gcgatacacc aggcgcagct gcagcgggat gatcgccatc
      721 gaggccatcg actgcggcag cagctcgagc gcgccgttga ggatcgacgc attcaggatg
      781 gtccggtcga gcgctgccgc atcgggggcg gcggcggcgg acggcgcagc cgccggctcg
      841 tcctcgccca ggccgggaag gctggcgagc ggcccgtcgg ccggcgcctg gccgagcgct
      901 tccgcctcgc gcacgaaggc gtccgcagcc ggccgcgaga gttccagcaa gccggacagg
      961 cgctcgagga agtccttctc gcgcgcatcc agcccaccat cggcctcgca caccccgacc
     1021 gccagctcga aggcgagctg gcgcaggttc gcctcgccca gcccggccac cgccgcctcc
     1081 aggccgagcc cgccgagcag gacgtcctgg tagagcgcga tgaagtcgat ctccgcctcg
     1141 cgcccgagcg attcggcgac cttgcgcaca tggtcgcgct ctaccgcccc ctggtgaccg
     1201 tcggcgaagg ccgcatgcag gcagatcgcg acgagggaac gggcttgctg tggattcagg
     1261 ttcatggacg gactccgcgg tggaaaacat tcaggttggg gatgggcggt ggcgcttcaa
     1321 gcgcgcatga cacgctgtaa cgattcgacc ggcgcggcgg cgggacgatc cccccgtgct
     1381 gcgagcacgg cgcgccgatg ctggccgccg ccggccggca cggacataat tacgcattac
     1441 cgcactccag gcccgcacac accatgtccc agtcgatcga acatctcttc gccaacaaca
     1501 agaactggtc ggaacgcatg cactccgagg accccgagtt cttctcgcgc ctggtcaagc
     1561 agcagtcgcc ggaatacctg tggatcggct gctccgacag ccgggtgccg gcgaaccagg
     1621 tgatcgggct cgcgccgggc gaggtcttcg tgcatcgcaa catcgccaac gtcgtcgtgc
     1681 ataccgatct caacgcactc tcggtcatcc actacgcggt cgagatcctg cgcgtgaagc
     1741 acatcctcgt cgtgggccac tacggctgcg gcggcgtgaa ggcggcgatg aacgacaaca
     1801 agaccggcct caccgacaac tggctgcgcc acatccagga cgtgcgcgac cgccacatcg
     1861 atcgcctgga gcgcctcacc gacccggccg agcgcgccga ccgcctgtgc gagctcaacg
     1921 ccatgcacca ggtggtgaac gtgtgccaga cctcggtgct gcgcgaggcc tggcagcgcg
     1981 gccagggcgt caccgtgcac ggctggtgct acagcctgca ggacggcctc gtgcgcgacc
     2041 tcggcgtcag cgcgggcacc cgcgaggagg ccgtggagcg ttatcgcgac gcggtcgagc
     2101 gcatcggctg acgctgcccg ccacggcatg gggacccgca tcggcatcga cctcggcggc
     2161 accaagatcg agctcgtggt gctcgacgcc gacggtcgcg agcgctggcg ccgccgggtg
     2221 ccgaccccgc agggcgacta cggcggcacg ctgcgagcca tcgccgcgct ggtcgaggag
     2281 gccgagcgcc tcaccggcgc gggcgcccgc atcggcgtcg gcacaccggg gtcgccttcg
     2341 ccgcgcgacg gccgcatccg caacgccaac tccacctgcc tgaacggcca gccgctgcag
     2401 caggacctcg aagccctgct gcgccgaccg ctccgcctcg ccaacgatgc caactgcctg
     2461 gccatgtcgg aggccgccga cggcgccggc gcgggtgcac gtaccgtgtt cgcagccata
     2521 ctcggcaccg gggtgggcgg cggcatcgtc gtcgaccaga aactgctggt gggcgccaac
     2581 gcggtggccg gcgaatgggg ccacaacccg ctgccgctgc ccgcgcccga cgacctcccc
     2641 ctgcccgcct gctattgcgg acgcgccggc tgtatcgaga cctacctgtc cggtcccggc
     2701 ctggccgccg atcatctgcg ccatgggggc gagcccctcg acgctgcagc catcgcccgc
     2761 caggccaccg agggccagcg cgcctctcag gccagtctcg aacgctacga gctgcggctc
     2821 gcgcgcgcac tcgcgggcgt gatcaacctg ctcgaccccg aggtcatcgt gctcggcggc
     2881 ggcctgtcgc agatcgcgcg cctgtacgag cacgtaccgc ggcggtgggc tgcgcacgtg
     2941 ttttccgaca ccgtgtccac gcgcctgctg ccggcacggc acggcgacgc ctcgggcgta
     3001 cgcggggcgg cctggctgtg gccgtcgaac tgagcgcgtc gagcgggaat tctcccgggc
     3061 agcgcggcac ggtgtgaatc gcgactggcg tcgctcctac aagggcctgc ggaggcgcgg
     3121 cggtgcgaat cgcgactggc gtcgctccta caggcgcctg cgggggcgcg gcggttttct
     3181 gtaggagcga cgcaagtcgc gattcagccg ccaggatcct cacgcagctg gtcggcacac
     3241 gacgccggcg accgagacga acattcccgc ctggctgccc tgacgaacca tcaccctgcc
     3301 tcagacctcc agcggatcca cgtccagctg ccagcgcagc tcgcgcgcgg tgcgctggcg
     3361 gtagagcacc gcgacccact ccgcgaggaa ggcctgcagc ggaccgcggt ggtcggcctc
     3421 gacgagcagc tgcgcgcgtt cgcggcgggc gagccgggtc atgcgcatcg gcaccgggtc
     3481 gtacagacgc agccccgcgg gcgcgcgctc ctcggcgagc cggcgcgcat ggcgcaggaa
     3541 ctcgacggca tcctccagcg ccggcgcatc ggcacgcagc atggcctggt gggtgaaagg
     3601 cgggaagccc gcggcacggc gctcctgcag ggccatgcgg gcgaaggcgt cgaagtcgtg
     3661 cttggccagg tgcagataga gcgggtgcat ggggtattcg gtctggatca gcacctctcc
     3721 gggcaacgcg ccgcgacccg cacggccgcc gacctgcatc agctgctgga acaggcgctc
     3781 gggggcgcga aagtccgcgg catgcagcga ggcgtccgcc cccaccaccc cgaccagggt
     3841 gagcttgggg aagtcgtggc ccttcgccat catctgggtg ccaaccagga tgtcggcctc
     3901 gccggcggcg atggtgtcga gcagcgtctc ccactggctg cgcgtgcgcg ccgcgtcacg
     3961 atcgacgcgc agcacgcgcg cctgcggaaa gagttcggcg aggcgcgcct cgatacgctg
     4021 ggtgccgcgg ccgaagggct ggatgtcctg gtcaccgcag ctcgggcagg cgtgcgggat
     4081 cggaccgtcg cagccgcaat gatggcagcg caggcggcga tcggcgaggt gcaccacgag
     4141 gttggccgag caatgcgggc agcggctgac ccagccgcag gacggacacg acagcaccgg
     4201 cgcgtagccg cggcggttga gaaagaccag gctctgctcg ccgcgctcca ggcgctgcgt
     4261 gatcgcgaca tgcagcgccg ggctcaggcc ttcgtcgagc ttggtcctgc gcgtgtcgat
     4321 gcagcgcacc gccggcagcg tggcggcgac cgcacgccgg ctcagcctct gcagcgcata
     4381 acgctcgctg cgggcgtgga accagctctc cagcgagggc gtcgccgagc cgagcaccac
     4441 cggcacgtcg cgctggcggc cgcgccacac cgccacgtcg cgcgccgagt agcgcacgcc
     4501 ctcctgctgc ttgtacgagg catcgtgctc ctcgtcgacc aggatcaggc ctagccgcgg
     4561 cagcggcgcg aacaccgcaa ggcgggtgcc gagcacgatg tcggcacgac cgctgagtgc
     4621 ctgcacgaag ccgcgcgaac gtgcaccgtc ggcgagcgcg gaatgcaggc tgaccacgtt
     4681 ggcggcggca aagcgctgcg ccacgcgctg ctcgagctgc ggcgtcaacg cgatctccgg
     4741 gaccagcatc agcacctgac ggccctgcgc gagcgcgtgc gcggcgaggc gcagatagac
     4801 ctcggtcttg ccgctgccgg tgacgccatg cagcagccag ggccggaaac gcatcccggc
     4861 accgacgatc gcgtccaccg cggcggcctg ctccggcgtc gccagcggct gcgcgccggc
     4921 ttcgtgacgc gcgccggggt cggccacgat ctccagccag ccacggcgca gcagttcgcc
     4981 caccgcctcg ccaccctccc gggcacggat cacgctgcgc cgccacggcc ccggctccgc
     5041 agcgagcgcc tgcagcagcg cgagcgcccg gctcgcccgc cgcgccgcgc aaagcgcctc
     5101 gccgcccttc ggggcgacgc caagcagcgg atcctcgtcc tcgtcactga cgccgtcggc
     5161 gcgacgcagg ccaggaggca gcgcgagcgc caccacctcg ccgagcggcg cgtggtaata
     5221 gcgcgccacg aaggacacca gctccagcca gtccgcaggc aaggccggca cgccgcgctg
     5281 gatgtgcagc acgggcttga tccgtgcgag ttccaccccc gcttcgtcgc cgataccgac
     5341 gatcaacccg gtgcgttcac cgcgcccgaa cggcaccttc acgcagcgcc cgacgtctcc
     5401 cacgcccgca tccgcggccg tgtaatcgaa ggcttgcggc agcgggatcg gcagggcgac
     5461 gcggacgatg gccatggagc ggcggaggct ggagaaaaaa ttgttcttct tcagcgactt
     5521 gtacgacttc gctgagagcg ggccgagaaa acggcgggca agtggctggc gggaaatgag
     5581 gcttccagct tgtccacaaa acctgtggat aagtttgtgg gaaagctggg tgaatcgggc
     5641 tcaaacctgc gccgcacaag gggtttcgtc acaatgcctt aacattgcgc gaatttttct
     5701 attttatttt caatgactta tgaaatctat cgggcaaagc ccgttatggg cggcattccc
     5761 gggcagaacc cgtgaaatgt gcataagtca agcccggaaa acacgaattc gtgcttgaca
     5821 gatggcggcg gaggcctgtc ctgcagcgca ccgccgcccg cacgcctcaa gccccggcgc
     5881 ggatcgcccg gctgtgcgcg tgcacggtgt ctaccagcac gctcacgctc tccggagggg
     5941 tgtactgcga gatgccatgg ccgagattga agacgtgtcc cggatggttg ccgaaggcat
     6001 cgagcacccg gcgcgtctcc gtcgcgaccg cctcgggcgg ggcgaacaac acgttggggt
     6061 cgaggttgcc ctgcagcgcc accttgtcgc ccaccaggcg acgcgcgcgg ccgatgtcca
     6121 tggtccagtc gaggccgacc gcgtcgcaac cgatcgcggc gatcgactcc agccacaggc
     6181 cgccgccctt ggtgaacacg atgctgggca cgcgctggcc gtcgcgctca cggatcaggc
     6241 ctgctacgac ctgttccagg taaggcagcg agaactcctt gtacgcggcc tcggacagca
     6301 caccgcccca cgagtcgaac accatcaccg cctgcgcgcc ggcctcgatc tgcgcgttga
     6361 ggtacttcac caccgcctgc gcggtgacgt cgaggatgtg gtgcatcagg tcggggcggc
     6421 tgtaggccag cgacttgacc ttgcggtagt cgtcggagga gccgccctcg accatgtagc
     6481 aggccagggt ccacgggctg cccgagaaac cgatcagcgg cacgctgccg tcgagcgcac
     6541 ggcggatctc ggcgaccgca tccatcacgt actgcagctc ggcgtggggg tccggcgcgc
     6601 tgaggttgcg gatctcccac tcgtccttga gcgggcgctc gaagcgcggg ccctcgccct
     6661 cggcgaagta caggccgagg cccatcgcgt cgggcacggt gaggatgtcc gagaacagga
     6721 tcgcggcgtc gaggtcgtag cgggcgagcg gctgcagggt cacctcgcag gccatcgccg
     6781 ggctcttgca cagctgcagg aagctgcccg cgcgcttgcg cgtctcgcaa tattcgggga
     6841 ggtagcgccc ggcctggcgc atcagccaga cgggggtgta ttcggtgggc tggcgcagca
     6901 gggcgcgcag gaaggtgtcg ttcttcaggc ggctcacgtc gagtcctcta tcggggcgtt
     6961 catgcacaag ccgggattat cgcctgtccg gcccccgaac gggcttgtcg tcggtcaagc
     7021 agtcgcggca gggccgctca gcgccgccag aacgtggggg tgagcaccac caggaaggtg
     7081 aagatctcca ggcggccgag gatcatcgcg aaacacagca cccacttgtg gaggtcgtcg
     7141 agcccctggt agttggacgc cggccccacc aggccgagcc ccgggccggt gttgttgagg
     7201 caggccacga ccgccgagaa cgccgtcacc agctccagcc cggcagcgga gagcaccagg
     7261 gtcaggctga cgatgctgac catgtacatg aagctgaagc cgagcacggc gtgcaggatg
     7321 tgctcgggca ccgggttcct gcccatgcgc accgggcgca ccgcgttcgg atgcagcgag
     7381 cgcacgatct cgcggatgac ctgcttgtac aggatcatcg cccgcatcat cttgatgccg
     7441 ccgccggtcg agcccgaaca ggcgacgaag ctgcccagga agaggatcca gatctgcgcg
     7501 aacatcggcc acaaggtgta gtcgtgcgtg gcgagcccga gcgaggtcga gatcgagacg
     7561 acgtggaagg cgacgtagcg cagcgcctcg ccgaagtcgg tgaagatgcc ctgggcgagg
     7621 aggtacgcgg tgagcatcag cgtggagagc gcgaggacgc cgaaatagaa ggggatctcg
     7681 ggatcgcgcc gataaggcgc cgacgagcgc cggaccagcg cgagatagtg ggtcgcgtag
     7741 ttgagtccgg cgagcaacgc gaagccggcg gtgaccagct ccaccccgac gctgtcgaaa
     7801 tgcccgagcg aggcgtcgcg gttggagaag ccgcccaggc cggcgacgga gaacgcgtgg
     7861 atcaccgcat cccagggcgc caaccctgcc caccacaggc tgaatccgca ggcgatggtc
     7921 agcaacacgt aggtcagcca cagccccttc gccgtctcgg cgatgcgtgg ggtcaggctg
     7981 gagtccttca tcggcgtcgg cacctcggcc ttgtagagct gacgaccacc gatgccgagc
     8041 agcggcaata tcgccaccac gagcacgatc acccccatgc cgccgaccca gtgcatgaag
     8101 gtgcgccaca ggttgatcga gatcggcagg tgctcgagcc cggtgagcac cgtggcgccg
     8161 gtcgcggtga gtccggagac cgcctcgaag taggcgtcgg tgaagccgag atcggggatg
     8221 taggccaaca gcggcagcgc gccgaagagc ggcagcacca cccaggtcgc cgccaccagc
     8281 aggaaaccgt catgcacccg cagatcgcgc cgcttgctgc gcgtcgccgc ccacagcagc
     8341 aggccggcga cgaaggtgac gaacaggccc tggtcgtagg ccagggtcgc gccgtccccc
     8401 agcgcggccg acacgacgag cgggaagccc atcagcagcc cgaagatcat gatgatcagg
     8461 ccgagggcgt tgagcgcggg gtagtacgcg cgggcgcggg cgtcggacga catgtcagag
     8521 gaagtggaag ccgacctgga acagcttctc gaccttctgc accagcttct tgttggtgca
     8581 gaacacgatc acgtggtcat cctcgcggat caccgtgtcg tggtgcggga tgatcacctc
     8641 gcggcggacc accctgccct cctcgttctt ctcgtcgcgc accaccgcgc cgatgctcgc
     8701 gccacgcggc agggcgatct gctcgatcgg gcgcccgacc accttggagt ccttgctgct
     8761 gccgtgggcg atgatctcca gggcctcggc ggcgccgcgg cgcaggctgt gcaccgcgac
     8821 cacgtcgccg cggcgcacgt gggcaagcaa cgtcccgatc gaggtgtgcg cgggcgagat
     8881 cgcaatgtcg atcggtccgc cctgcaccag gtcgacatag ctcttgcggt tgatgagcgc
     8941 gagcgtgcgg cgcgcgccca tgcgcttggc gagcgaggcg gacatgatgt tgtcttcctc
     9001 gtcgttggtg agcgcgacga agaggtcggt ctcgtcgatg ccctcggtct cgagcagctt
     9061 ctcgtcggtg gagtcgccgc gcagcacgag cgcattcgac agatgcgagg cgagcatctc
     9121 ggcgcgggtg cgattgagct cgaggatctt gacgtggtgg ttctcctcga tcgactgcgc
     9181 gagccggaag ccgatgttgc cgccgccggc gatcatgatc cgccgcatcg gccggtcgga
     9241 gcggcgcagc tcgcgcatca cctggcggat gtgcaccgtg gcggcgaggc agaacacctc
     9301 gtcgccgggc aggatgatgg tgtcgccctc cggcatgatc ggctcgccgt tgcgatagat
     9361 cgcggcgatg cggacctcga cgttgggcag gtggaagcgc agcgccttga gcggatggcc
     9421 gaccagcggc ccaccctcga aggcgcgcac gccgatcagg ctcaccacgc cgtcggcgaa
     9481 ctcgagcacc tgcagggcct cggggaaggc gatcaggcgc ttgatgtagt cggtgacgac
     9541 ctgctcggga cagatggaga aatccaccgc gaagttgtcg tcgtcgagca gttcgggatg
     9601 gtcgacgtag tcggtcgagc gcaagcgcgc gatccgcgtc ggcaggttga acagcgcctt
     9661 ggcgatgcgg caggcgcaca ggttggtctg gtcggactgg gtcaccgcga tgagcaggtc
     9721 ggcatcgtcc gcgccggcct cgcgcagcac tcgcggcgag gcgccgttgc cgcacacggt
     9781 gcggatctcg aggcgctcgc gcagcgcgcc gagatactcc tcgctggtgt cgaccagggt
     9841 gatgtcgttc gcttcggaga cgaggttctc ggccaccgag gcgccgacct ggcctgcacc
     9901 cagaatgatg atcttcaacg ttgtatccgg aagtgtccaa gccgccgatt ctacgccccc
     9961 cacccggccc ggcaaacgca gacgttgcgc cgcagcaacc gccggatcct gccgagccgt
    10021 ggcgcagcgg caggagagcg cgcaggcttc aggactcctc gtgccggcgc ccggcctgca
    10081 ggccgagttg cttgagcttg cggtagaggt gggtgcgctc caggccggtc ttctccgcca
    10141 ggcgggtcat gttgccgccc tcgaggcgca gatggtgttc gaagtacatg cgctcgaagg
    10201 cctcgcgcgc ctctcgcagc ggctggtcga gcgacacgcc gcaggcgccg gcgcccagat
    10261 ccggtggcag caggcgacgc acttcgccgg cgccgatctc ctcctccagc gtgccgagcg
    10321 cgagcgactt caccgcggcg cgcaactcgg ggtagccccc cggccacggc aggttgcgca
    10381 gctggttgag cgcggcggtg gaaaagcgcc gcagcggcac ttcgcccgcc tccaccagat
    10441 gcagcagcat ctggctggcg agctcgggca ggtcctcgcg gacgtcggcg atcgagggcg
    10501 gcgcgaggct cacctcgaac aggcgggcga gcagcccctc ctcccagccc tcgccgacca
    10561 gggcctcgag actgcggtcg gtggcgacga cgaggcgcaa gtcgtagcgc tcgaggcggt
    10621 cgagcgcgaa ggcgaggttc ttttgctgcg cgcgcgacag gcgggccagc tcgccgacga
    10681 acaccacacc gccctgggtg gactgcaact gggtgatgtc gagcggcgcg gtgacggcgg
    10741 cgaggtcgag ccagggggcg ttggctggtt gcacgctgcg tgcgaccagt tcggcgagcg
    10801 accccgcgcc gacgcgcagc agcagcacgc gagaactgcc ggcgatctgc tcgagccggc
    10861 ggcgcagctc gcgcaagggc acggagcgat gaaaggcggc gagcgtcggt gccgactgca
    10921 cgccgggcgc ctgcggacgc tgcagcccgc gcttgaccgc ggcgagcagc ttctgcagcg
    10981 cgatcggctt ttccagatag tcgatcgcgc cgatgcgggt ggcctcgacc gcggtgtcga
    11041 tggtgccgtg ccccgacatc atcaccaccg gcatgttgag ctggccattg gccgcccatt
    11101 ccttgagcag ggtgatgccg tcggtgtcgg gcatccagat gtcgagcaac accatgtccg
    11161 gacgcactgc gttgcgtgct gcgcgcgcgg cggtggcatt ctcggcgagc aggatgtcgt
    11221 gcccctcgtc gcggaggatc tccgagagca gttcgcggat gccgatttcg tcgtcaacga
    11281 tcagaatctt tgccattttt cttcgtcatg cttccttgtt ttcggccagc cgcaggcgga
    11341 tgcgcacttc ggcgccaccg ccctcgcggt tgctgaggcg gacctcgccg ccatgctcgt
    11401 cgacgatctt cttgaccatc gccaagccca ggccggtgcc gcgcgacttg gtggtgaaat
    11461 agggctcgaa ggcgcgcgcc aggacttcgg gcggaaaacc cgggccattg tcgcgacaca
    11521 gcagcagcgc gctctcgccg tcacgacggg tgatcaccga gatctccgcg tcctcctgcg
    11581 cgccaagcgc gtcctgcgca ttttgcagca tgttgtggat cacctgccgg atctgcgtcg
    11641 gatcgccctg caccggaggc agcgctcccc ccagctcggt gcggatctgc accgcggcac
    11701 tctcgtacag gtggagcacc tcgcggatca gcgcgttgag gtcgagcggc gcgagctggg
    11761 gcgccggcag ccgcgcatag tccctgaagg cgttgaccag gttcttcatc gcctcgacct
    11821 gattcacgat ggtggtggtt gcgcgctcga gcatctcacg cccgttgtcg tcgaggcgat
    11881 cggcgagctt gaaggagaga cgctcggccg aaagctggat cggcgtcagc gggttcttga
    11941 tctcgtgcgc gagccggcgc gcgacctcgc cccacgccgc cgtacgctgc gcggcgatca
    12001 ggctggagat gtcgtcgaac accaccacca agccgccccc cgtgctctcg ggcaggcgcg
    12061 agccgcggat cagcagggtg cgcggcgggc ggtccctgac cggcaactcc atctgcttct
    12121 gccagtctcc cgtgttggcg gtgaagccct cgcgcagcat gtggccgagc tcggcctggc
    12181 gtggccaggc ctcgagcggg atgtcctcga atccgcccag atcatcctcg aggatttgca
    12241 gcgcaccatg gttggcggcg cgcaggtgac cgtcggccga gaaggcgagg acgccggtgg
    12301 acagattcgc cagcaccgac tccaggtagg cgcgcgcagc ctcgacctcg gcgcgattgc
    12361 ggtcggccgc cgcgcgcgcc tcctgcagct ggcgcgtcat gcggttgaag gactgcgtca
    12421 gcacgccgag ttcgtcgttc gccggcaagg cctggcgcgg gctgaaatcg ccctgcgcca
    12481 ccgcctgcgt gccctcggcc aggatcagca gcggttgcgc caatcgacgc gcgatcagca
    12541 ccgcgaccgc gagcgcgcca agcagcgcga gcagcagggt caggctcaag gtgaccgtgt
    12601 agatgcgctt gagcccgctg cgtccgagcg tcagctgctg gtactcgcgg tatgcctcct
    12661 ggaccgcctc ggcattgcga ccgaaggatt cggggaccgg ctgctgcagc atcagcagat
    12721 tggactcgcc cgccagcgtg cgcaccggga tcggcgtcag cacgcggatc accaggcgac
    12781 cgtcgggttc ggcgatcaca gaatgaaaat ggcggctctg acgggcctgt cgcagttcgg
    12841 ccgtgcccgg aagctcggga agcagcgtcc gccccgactc ggccgcggtg gcgagcacct
    12901 ggcccgcggg ggcaaggatg gtggcgctcg cgatcccggc ctgctcgcgc agccgattca
    12961 gccgggtacc cgcgccctgc ccggccccct cgagctccag caccatgtcg tccgccttgt
    13021 ctcgcacctg gctcaccagg taatcgagcg cgttctggcc gagcgcgatc ccgccttcga
    13081 gcgccgaatc gacccgcacg tcgaaccagg attcgatgct gcgcaccacg aactgcaggg
    13141 acacgccgta gatcaccagc cccggcacca cgcccatcag cgcgaacatc agcaccaggc
    13201 gcatcttgag acgggaaccg aactgcccct cgcggtgctc gcgcagcagg cgatgcagct
    13261 gcatgccgac gagcgcggcg aggaccacgg ccatgccgcc gttgagcgcg agcaaccaag
    13321 gatagctggt cgcgaacagg gtggtgttcg aactcgccga ggtgagcagg aacaacgaga
    13381 tccccgacag cgctgcggcg acggcgagca ggacgcgctt catcggaacc ccggcgcccc
    13441 cggcaggaag gtccagcgca cccactcggt ctcgacgttc cagtcgttgc tgccgatcgc
    13501 ggtgacctga aagggcttgg gaagctggct ggtgtcgagc cggaagcgaa gggaaacctc
    13561 gtgcgagacc ccggactgga ggacgccggt ctccgcgaca agccagttgc gcacgcgctg
    13621 catggtgcgc agcgcctcgt cgagttcgtc gaagttctgg tgcagattgc cgaccgagag
    13681 ccggaagctg cgcgtgatcg cgtggtaata gagccggtag tccaggctac gacgcgccac
    13741 cgtctcgttg agccagtacc agcgcgggcg ctcgatcagc acctcggtgg tgaagtaaag
    13801 cgagacgccg cgcgtgacca cgtcgatcag gcggggattg agatcgatcg cgatatccgc
    13861 gttgaggaca taaccgccgt caccgggcac gatctcggcg ctgcggatga cgctctccgc
    13921 gcgtacgctc agcgcccaca acaagagcag cgcggccacg gcgaagcgaa cggcgcgctc
    13981 agggccgctt gcggaggagc gcgtaataga agccgtcatg gtcggcacaa ggcagcagct
    14041 gcaggtcgga agatccgcga atcgggagtc gctccgcgtc ggcatggcgg acgcagaagc
    14101 gggcgacctg gccggcgttt tcctcgtcga acaccgagca ggtgacgtag agcattgtgc
    14161 cacccggggc gagcgtgcgc caaagtgcct ccaggatttc cgcctgctgt gcggcaaagt
    14221 tggcgatatc gctgtcccgg cgcaaccact tgatgtccgg atgtcggcgc accacgccgg
    14281 aggccgagca cggcacatcg gccaggatgc ggtcgaaggg acgaccatcc caccacgctg
    14341 ccaggcggcg gcagtcggcg accttcagct ccgcgcgcag gccgaggcgg tcgaggttgc
    14401 gcgccacccg acccgcccgc agcggatcaa gctccagcgc gagcaggtcc gcatcggccc
    14461 tctcgaggat gtgtgcagtc ttgccgccgg gggctgcgca ggcgtcgagc acacgctcgc
    14521 cggctcgagc atcgagcagt tgcgccgccc actgtgcccc cgcgtcctgc accgacaagc
    14581 gcccttcggc gtacccgggc aggcgtgcga ccgccagcgg acgcgcgagc accagcgcgt
    14641 cgttgtcgag tcgccggaac tccagcccgg ctgcggcaag ctccgcctcg acctcggcca
    14701 acgtggcacg gcgcggattg acccgcaagg ccatgggggg acgcgtattg ccggccgcga
    14761 gcagatcctg ccaggcctga ggatgcgcgc tgcgcacacg ctccacccac caggccggaa
    14821 aggcatgacg cgcctcgggc tcggcctcga tccagccttg ccactccggc tgccgacgca
    14881 gcgcattgcg cagcacaccg ttgaccaggt tgcgcaggcc gggcatcgcc acggccgtgg
    14941 cgcccacagc ctgatcgacc accgtgtggg cttgctcggg gcgctgcgtc aggcgctgca
    15001 gggcaaccag cagaagcgcc cgaatctcca ccggaggagg actatgcagc aggcgggaaa
    15061 ggatcgcatc accgcgcccg aactcgcgca aggtcgacca ggcgaggtcg cgcacggcgc
    15121 cacgcgtgcc ttccggccat tccggatgcc cggcctgcat gcgctcgaag gcatcggtca
    15181 ggttggcgcc gtcgatcacg ccggcgacca gttcggttgc ccgtgcgagc gcgtagccga
    15241 ggctgtcgat gggctgttcc ggagcacggc gtggggcacc acgcacaccg gctgtgcggg
    15301 agcggggcga gccggcggcc ggcagggcgg accgtcggtc tttggggtgg gctggggtca
    15361 aggtctggag aacggccgaa gaagcggcac tggctcaggc cagcaaggca cgcagatgat
    15421 tgggcagggc gaactgcgca cagtgtaccg caccattgaa gtgctgcagt tcgcggatgg
    15481 cgcgctcggc aagacggcgg tccacctcgt tcgcgtcgag cgcagccggg tcgagcgtat
    15541 cggacgcgca ggccatgccc cagaggctcc catagagcgg aatgtagagg aagtacggcc
    15601 gcacccgcgc aaacacccga cgcaggcggg cgagcaactc tcgcacacgc tcgggctgga
    15661 ataccggcgt accgatatgc agcgtcagcg ccccgccctc ggccagcagg gccttgcagg
    15721 ctgcgaagaa gtcggcggtg tagagcgctt ccgccggccc gatcgggtcg gtcagatcga
    15781 gcacgatcag gtcgtagcgc tcgcccgtgg cgggagcatg ctcgcacaca tgggccatcc
    15841 cgtccccgat gtggatctcc acgcggggat cgtcgagggc gccatgatgc acggcatcga
    15901 ggtagcgccg ggcgatgtcg accaccttcg cgtcgagctc ggcgatcacg atacgctcga
    15961 tgcccggata cttgagcagc tcttcggccg aaccgccatc ccctcccccg atgagcagcg
    16021 cccggcgcag gccgggatgg gcaaccccgg ggacgtggat caggttctca tggtagtgga
    16081 actcgtcgcg ctcggacgtc atgaaacagc catcgaggcg aaagagcttg ccgaagcgcg
    16141 gcgtttccca gacctcgtag tcttgccact cggagcggcc ggactcgagc aggcgcgcac
    16201 cacggagaaa gtagccggca tcctcgccga ggagctcggc gaggacatcc ttcggcagat
    16261 cggcaaacca ggacgctccg ctcatgatct tctgcactcc acagctttcg
//
LOCUS       DAOQBS010000064        16202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2680_length_16202_cov_14.020313, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000064 DAOQBS010000000
VERSION     DAOQBS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 16202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16202
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2680_length_16202_cov_14.020313"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1385
                     /locus_tag="PKN25_08810"
     CDS             288..1385
                     /locus_tag="PKN25_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HNV91040.1"
                     /translation="MTALFQPFKLKDLTLRNRIAIPPMCQYSATDGVVNDWHRVHYPA
                     LARGGAGLLIVEATAVSPEGRITPGCLGLWNDTQAAALAPIAASIKAAGAVPGIQIGH
                     AGRKASANRPWEGDDHIAADDARGWETLSPSATAFGAHLPKLPREMTVDDIARVRADF
                     VAAAKRARAAGFEWLELHFAHGYLGQCFFSVHANQRSDAYGGSFENRSRFLIETVRAV
                     RAVWPENLPLTVRFGVIEYDGRDEETLAESIELARSFKREGLDFLNVSVGFSTLKARV
                     PWGPAFLAPVAERVRREAGLPVAAAWGIDNPVIADACVRDGQLDLVMVGRAHLANPHW
                     PMHAARALGVERPTWVLPAPYAHWLERYAGA"
     gene            complement(1457..3025)
                     /locus_tag="PKN25_08815"
     CDS             complement(1457..3025)
                     /locus_tag="PKN25_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HNV91041.1"
                     /translation="MSKSKVIPAAEAGKLIKDGATLYCSGVGLAGFAEEVAISIRERF
                     EATGHPRDMTIWHACGLGNGKNKGIFHLTQPGMIKRIVGGHFGVGGPVLMQLIMDSRI
                     EAYNLPQGVLVVLPREMAAGRPGLITKVGLETFVDPRIEGGKLNSATSEEIVERVDFD
                     GQEWLFYRAPRIDVALIRGTTADENGNLTVEDEGIFNESISIAQAAKRNGGIVIAQVL
                     QVVQNGTLHPKQVKVPGILVDHVVVGRPENHMQTMGTQYNRAFAGDIRVPVGSLRRLP
                     LDERKIIARRAAVELGHGAIVNLGIGVPAGIASIAAEEGVQDLMALTLETGPIGGIPA
                     DGLDFAHASNADATIDPGYQFDFYDGGGLDVAFLGLAQTDVHGNVNVSKFSGRPVGCG
                     GFINITQNAKKVVFCGAFTAGGLEIRAADGRLEIVREGTSRKFLDHVEQITFSGHYAA
                     GVGQTVMYVTERAVFQLTREGGMELLEVAPGVDIERDILAHMDFVPRMERVREMDAGI
                     FSERWGRLYEILHS"
     gene            complement(3083..4870)
                     /locus_tag="PKN25_08820"
     CDS             complement(3083..4870)
                     /locus_tag="PKN25_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNV91042.1"
                     /translation="MYDYQPPLRDMQFILAELAGLDDIARLPGCEEASADVIGAVLEE
                     AGRFAAGVLAPLNRAGDEQGCRLLEGGRVATPEGWQQAYAQFCAAGWLGLSLPVEFGG
                     QGLPKLVSTPVMEMWNAANMAFTMLPILNQGQAEALRIAASAEQKRTWLPKVVSGEWG
                     STMNLTEPQAGSDLAATRTRAEPQADGSYKLFGQKIFISYGEHELTPNIVHLVLARLP
                     DAPAGVKGLSLFIVPKFLVEPDGSLGARNDVRCISIEHKMGIHGSPTCTLSFGEGGGA
                     TGWLVGEPNRGLEVMFVMMNEARFGVGVQGAALAERAYQMALGYARERVQGRDAVSGA
                     AGRPILCHPDVKRMLLSMRARVMAMRALLYTAAGWFDIAHHHPDAAVADKHRRYVDLL
                     MPVAKGWCTELGNDVCDEAIQVFGGMGFVEETGVAQHFRDARIITIYEGTTGIQANDL
                     VGRKILREGGATLRELIVELRASATALSAAGLAELGDGQGAAIDALESATDWILANGR
                     EQLGEVLAGAVPFLHLLGTVCGGWQLGRAALAARARQAAGEGDPVYLDGIVELARFYM
                     ATLGPQAAAHAATVREAGGALTRFDEAAF"
     gene            complement(4908..5327)
                     /locus_tag="PKN25_08825"
     CDS             complement(4908..5327)
                     /locus_tag="PKN25_08825"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNV91043.1"
                     /translation="MARRHLHRMRIAFSDCDPAQIVFFANYFKWFDTAFREFCTACGV
                     PSWRETTATRGIIGAPLVDAQAKFRNSATYGEDIEIESWVEDWGGKSFVMRHVARRGD
                     TVLCEGREVRVFAVQDPEDPLRIKAIPIPEDLRAACS"
     gene            complement(5341..6108)
                     /locus_tag="PKN25_08830"
     CDS             complement(5341..6108)
                     /locus_tag="PKN25_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HNV91044.1"
                     /translation="MRFAGNTFIVTGGASGLGEATVRALHGAGANVVIADLNAGQGEP
                     LATALGERAAFARTDVSAEADARAAVDLAVSRFGGLQGLVNCAGIGNAGKVLGKEGAQ
                     PLADFERSIRINLIGTFNMIRLAAEAMSKGVPQADGERGVIVNTASVAAYEGQIGQAA
                     YAASKGGIVSMTLPIARELARFGIRVMTIAPGLFITPMMRGLPPEVQKSLGENTPFPP
                     RLGEPEEFAKLALSIIDNVMLNGETIRLDGAVRLAAR"
     gene            complement(6129..7400)
                     /locus_tag="PKN25_08835"
     CDS             complement(6129..7400)
                     /locus_tag="PKN25_08835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNV91045.1"
                     /translation="MSKQGMGNFAESEAAARGLAYDDIWLVAGRRTPFADYNTVLRDV
                     SPTDLGIAAARALFADSGIPAAEVDAIVAGNMAQSSFDAYYLPRHIGLYSGVNPDVPA
                     LLVQRLCCTGFDTILTAADHITLGKANVVLCVGAESMSRNPVAAYTHRAGFRMGQVEF
                     KDFLWEATLDTAPKTSMGGTAENLAVRYGIDRDEVDRFAAQSFARGVAAWEAGWFGDE
                     VSALANETWELDGYKPRGIRLADRASVCDRDGHVRPTALEILQKLKPAFGGVQTGGNS
                     SAIVDGAAAVLVARGDWVRAHGLQPLARVVAGAAVAVPPEIMGIGPAPAIRAAARLAG
                     IAVEDIGRVEINEAFGAQYLACERELALDRDKANVHGGAIAIGHPLGASGVRLTHTVA
                     RAVRAAGLQYGVASACAGGGQGVAVIVENVA"
     gene            complement(7397..8167)
                     /locus_tag="PKN25_08840"
     CDS             complement(7397..8167)
                     /locus_tag="PKN25_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNV91046.1"
                     /translation="MTEQLVKTTSEDGIYTLLLARPDKRNAISDRLLAALEAALVATP
                     EGTRAIILAGEGKHFCAGLDLSEHQHRDAFGTMLHSQSWHRIFARIQNGGIPVIAAMQ
                     GAVIGGGLELATAAHIRVAEPSTIYQLPEGRHGIYVGGGASVRVAKIVGAGRMCEMML
                     TGRILDADEGQRLGLSHYLVGAGETLAKAQELARRVAENAPMANWAMVTAIARIDNMA
                     SDDGFFVESLTAAVVQTNPEIAARIGEFLQRKGQGARQ"
     gene            complement(8191..10068)
                     /locus_tag="PKN25_08845"
     CDS             complement(8191..10068)
                     /locus_tag="PKN25_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HNV91047.1"
                     /translation="MAEIIRDAARLFADPDLIIRHRPNGDRILRSGIELPDAIPRCVG
                     DWLEHWAAVQPDEVFLAERNAAGEWDRLSYGEARRRVVAIASWLLGQKLSAERPVVIL
                     SDNGIEHALLALAAMHVGVPVAPISPGNSLMSKDFGKLRGNFELLRPGVVFADPIERF
                     LPAIAAVADLHDGVVVAGSRSQPQPGVLPIAQIEAQAAAKADEAAVMAAFAAVGPDTI
                     AKFLFTSGSVGTPKAVINTQRMMCSNQLAKELVWPFLRSNRPVVCEWLPWSHTFGGNH
                     DFNMVLRWGGSFYIDDGKPTPALIEKTLRNLREVSPTIYFSVPRAYDMLVPHLRQDAA
                     LRERFFRRLQVIMYAGAALPQHLWNELEALSEASVGARIPMVSSWGSTETAPLATDCH
                     FQAVRAGVIGVPVPGTELKLVPSHGKLEVRVRGPNVFTGYWKQPELTAKSFDDEGYYL
                     IGDAVEFVDAAHPERGLLYDGRVGEDFKLLTGTWVHVGSVRVAGIDAMSPIAQDIVVT
                     GHDRDEIGFLIFPNLPECRRLCPHLAPDADIIDLLLNPAIRSRVAQGMAAMIKKGGGS
                     STHPTRAILMAEPPSVEAGEITDKGYINQRAVLTRRADLVEFLYADVLDKTVITVHPA
                     A"
     gene            complement(10108..11247)
                     /locus_tag="PKN25_08850"
     CDS             complement(10108..11247)
                     /locus_tag="PKN25_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV91048.1"
                     /translation="MKLAKLVVALSATFTLSSAAFADITIGVSLSATGPAASLGIPEK
                     NAFALLPTEIGGEKVKLVVLDDATDPTVATKNARRLVTEDRVDAIIGSSSTPATAAMA
                     EVAHETKTPLVTMSPVSLPVERNEWVFRTPQQNEVMASALVEHMKSKGYKTLGFIGFS
                     DAYGEDWLNSVTKHAGAAGIQLTVVERYNRNDTSVTGQVLKLTTARPDAVLVAGSGSP
                     AALPQGALVERGYKGQIYQTHAAANQAFLGVAGKAAEGAILPVGPVSVVRQLPDSHPS
                     KASGVDFVTKYEAQYGAGSFSAFAGYAHDAFRLIEAAVPAAKAKGAPGTAEFRAALRD
                     ALEASKEVVGVHGVFNMTPNDHFGLDDRSRVLIRIEGGAYKLVAQ"
     gene            11535..12428
                     /locus_tag="PKN25_08855"
     CDS             11535..12428
                     /locus_tag="PKN25_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV91049.1"
                     /translation="MNAARPDGAAMAPMTDTEAAILQLAREAPELGQVAVAERLRSEG
                     LRISPSGVRYLWQKHGLETAVKRLHALEEGRGAEALTEAQRRLLERGLLTERLARGEQ
                     AEDGAGKEQEPTERRRLILDAAARLFSAEGYDRTSIRDIARNVGLLPGSVYHYFPSKR
                     ELFLAVHREGFEHTLERVREAAASGGDPWECLRRACEAHVEGIVGGSSVDRLAGSNLA
                     TAQNDELLNEIRPHRAAYETVFKTLIDALPLRAGTDRSLLRLFLLGGMNWVYLWYREG
                     KRSPREIADAMVDMLRSGVQP"
     gene            complement(12454..12987)
                     /locus_tag="PKN25_08860"
     CDS             complement(12454..12987)
                     /locus_tag="PKN25_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNV91050.1"
                     /translation="MSLAHALLTSLLEQPSSGYELARRFDKSIGHFWQATHQQIYREL
                     GRMEKAGWIRSDADPDAGRTRKRSYSVLPTGRDELMRWAAEASPLPELRDEFMVRLRA
                     DAVTGPLGLLPELDRRRALHADKLRAYRAIEARDFPAEVEPTRQRRIHHLILKAGIQY
                     EQGWIAWYDEAIGVLGE"
     gene            13210..16113
                     /locus_tag="PKN25_08865"
     CDS             13210..16113
                     /locus_tag="PKN25_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNV91051.1"
                     /translation="MATYPNLFRPLDLGFTTLPNRFLMGSMHVGLEEAEGGVERMAAF
                     YAERVRGGVGLIVTGGIAPNLEGRPWSGGATLTTSEEAARHRVITDAVHREGGKIALQ
                     ILHFGRYAYHPELVAPSPIQAPIAPFVPRELSAADVERTIEDFVRCAELAREGGYDGV
                     EIMGSEGYLINEFIVAHTNKRTDAWGGAFENRIRFATEIVRRTRARLGREFILIFRLS
                     LLDLVEDGSTFEEVVQLAQAIEAAGATLINSGIGWHEARIPTIATCVPRAGFAWVTQK
                     LKDHVGIPLIATNRINTPEIAESILAEGKADMVSMARPFLADPDFVRKAAEGRGADIN
                     TCIACNQACLDHTFAGKITSCLVNPRACHETELVIEPTTAPRRIAVVGAGPAGLAFAT
                     TAAERGHKVVLFEAGARIGGQFNIAMQIPGKEEFAETLRYFGRRIEQTGVALKLNTRV
                     SAAELAGKFDEVVLATGIVPRVPEIEGVDHPKVLGYLDVLRDSKPVGRRVAILGAGGI
                     GFDVAEYLSHEGISPSLAPAKFYAEWGIDARYANRGGLTRPHLETAPREIVLLQRKAS
                     KVGEGLGKTTGWIHRTALKNRGVRMIAGVTYRRIDDAGLHVSIGGKDEVLAVDNVILC
                     TGQEPQRELQAALVEAGMRVHLIGGADVAAELDAKRAIKQGIELAASIEKAASAPALL
                     AGQLPASPGGAGIPLPQFDTLRIGLDGQVALVTLNRPDKANAMNLQMWQDLRAAMQWV
                     DRTPAVRVAVLHGAGANFCAGIDLQMMMGILPMVKDACEARTRENLRNLILDLQDTLT
                     SLERCRKPVLAAIHGACVGGGVDLVACADMRYCAAGTYFSVKEVDLGMVADVGSLQRL
                     PRLIGEGMVRELAYTGRRVDGAEAGRIGLVNRVFDTPEALMEGVMQLAQAIAAKSPLA
                     IRGTKDMLNHARDHSVADGLDRVATWNAAMLLSDDLQAAIRAGLTKQPPKFRD"
BASE COUNT         2353 a         5714 c         5715 g         2420 t
ORIGIN      
        1 agcttcgacg gcgtcaactg gacgccggac tcgacctgcc gatgcggctg ctgaggggtt
       61 tgccgccccg gtcgtccgcg ggcgaagtgc gaacggcccg agcgtcgcca ggcgccgggc
      121 cgttcgcttg tgcctttgga gccgaggcaa tgccgagtgg acgcgcgcag cgctcgtccg
      181 ctaccttcca gccctcgttc ggcccgtcgg ccgcacaatc ccttcgctca tctttcccca
      241 tccggccgcc acgcggcctg catcgcccct tcggaaccac atcgctcatg accgccctct
      301 tccagccctt caagctcaag gacctcaccc tgcgcaaccg tatcgccatc ccgccgatgt
      361 gccagtactc agccaccgac ggcgtggtca acgactggca ccgcgtgcac taccccgcgc
      421 tcgcgcgcgg tggcgccggg ctgctgatcg tcgaggccac cgcggtgtcg cccgagggcc
      481 gcatcacgcc gggctgcctc ggcctgtgga acgacacgca ggccgccgca ctggcgccga
      541 tcgcggcctc gatcaaggcc gcaggcgcgg tgcccggcat ccagatcggc cacgccggtc
      601 gcaaggccag cgcaaaccgg ccgtgggagg gcgacgacca catcgccgcc gacgatgcgc
      661 gcggctggga gaccctgtcg ccctcggcca cggccttcgg cgcccacctg cccaagctgc
      721 cgcgcgagat gacggtggac gacatcgccc gcgtgcgcgc ggacttcgtc gccgccgcga
      781 agcgcgcacg cgcggccggc ttcgaatggc tggagctgca cttcgcccac ggctacctcg
      841 gccagtgctt cttctcggtg cacgccaacc agcgcagcga cgcctacggc ggcagcttcg
      901 agaaccgcag ccgcttcctc atcgagaccg tgcgcgcggt gcgcgcggtg tggccggaga
      961 acctgccgct gacggtgcgc ttcggcgtca tcgaatacga cggccgcgac gaggagacgc
     1021 tcgccgagtc gatcgagctc gcgcgcagct tcaagcgcga gggcctggac tttctcaacg
     1081 tcagcgtcgg cttctcgacc ctgaaggcca gggtgccctg gggtcccgcc ttcctcgccc
     1141 cggtcgccga acgcgtgcgc cgcgaggccg ggctgccggt ggccgcggcc tggggcatcg
     1201 acaacccggt gatcgccgac gcctgcgtgc gcgacgggca gctcgatctg gtgatggtgg
     1261 gccgcgccca cctcgccaac ccgcactggc cgatgcatgc cgcgcgcgcc ctgggcgtcg
     1321 agcgcccgac ctgggtgctg ccggcaccct atgcgcactg gctggagcgc tacgcggggg
     1381 cttgaggcga agcagcggcg caggcggccg cggccgggtt tcaccccggg cggccgcccg
     1441 gggtcgagcc cgcgtctcag ctgtgcagga tctcgtacag ccggccccag cgctcgctga
     1501 agatgccggc gtccatctcg cgcacgcgct ccatgcgcgg cacgaagtcc atatgggcga
     1561 ggatgtcgcg ctcgatgtcg acgccgggcg ccacctcgag cagttccatg ccgccctcgc
     1621 gggtgagctg gaacaccgcg cgctcggtga cgtacatcac cgtctgcccc accccggcgg
     1681 cgtagtggcc gctgaaggtg atctgctcga cgtggtcgag gaacttgcgg ctcgtgccct
     1741 cgcggacgat ctcgaggcgg ccgtcggcgg cgcggatctc cagcccgccg gcggtgaagg
     1801 cgccgcagaa caccaccttc ttggcgttct gggtgatgtt gatgaagccc ccgcagccca
     1861 ccggccggcc gctgaacttg gagacattca cgttgccatg gacgtcggtc tgggcgagcc
     1921 cgaggaaggc cacgtcgagg ccgccgccgt cgtagaagtc gaactggtag cccgggtcga
     1981 tggtggcgtc ggcgttgctg gcgtgggcga agtcgagccc gtcggcgggg atgccgccga
     2041 tcgggccggt ctccagggtc aaggccatga ggtcctgcac gccctcctcg gccgcgatcg
     2101 aggcgatgcc ggcgggcacg ccgatgccga ggttcacgat cgcgccgtgg ccgagctcga
     2161 ccgcggcgcg gcgggcgatg atcttgcgtt cgtcgagcgg cagccggcgc agcgaaccga
     2221 ccggcacgcg gatgtcgccg gcgaaggcgc ggttgtactg cgtgcccatg gtctgcatgt
     2281 ggttctcggg acggccgacg acgacgtggt ccaccaggat gcccggcacc ttgacctgct
     2341 tcggatgcag ggtgccgttc tgcaccacct gcagcacctg cgcgatgacg atgccgccgt
     2401 tgcgcttcgc ggcctgggcg atcgagatcg actcgttgaa gatgccctcg tcctcgaccg
     2461 tgaggttgcc gttctcgtcg gcggtggtgc cgcggatcag cgcgacgtcg atccgcggcg
     2521 cgcggtagaa cagccactcc tgcccgtcga agtcgacgcg ctcgacgatc tcctcgctgg
     2581 tggccgagtt gagcttgccg ccctcgatgc gcggatcgac gaaggtctcc aggccgacct
     2641 tggtgatcag cccggggcgg cctgccgcca tctcgcgcgg cagcaccacc agcacgccct
     2701 gcggcagatt gtaggcctcg atgcggctgt ccatgatcag ctgcatcagc accggcccgc
     2761 ccacgccgaa gtggccgccg acgatgcgct tgatcatgcc cggctgggtg aggtggaaga
     2821 tgcccttgtt cttgccgttg ccgagcccgc aggcgtgcca gatcgtcatg tcgcgcggat
     2881 ggccggtggc ctcgaagcgc tcgcggatcg agatcgcgac ctcctcggcg aagcccgcca
     2941 ggcccacgcc gctgcaatac agcgtggcgc cgtccttgat cagcttgccc gcctcggcgg
     3001 cgggaatgac tttcgatttc gacattggtc cagttccaat ttttgcaccc ggcaaggcat
     3061 cggcctgccg ggcggacggt gatcagaagg ccgcttcgtc gaagcgggtc agtgcaccgc
     3121 cggcctcgcg cacggtggcg gcatgggccg cggcctgcgg gcccagggtc gccatgtaga
     3181 agcgcgcgag ttcgacgatg ccgtccaggt acaccgggtc gccctcgccc gccgcctgcc
     3241 gcgcgcgcgc cgccagcgcg gcacgcccga gctgccagcc gccgcacacg gtaccgagca
     3301 ggtgcaggaa gggcaccgcg ccggcgagca cctcgccaag ctgctcgcgg ccgttggcga
     3361 ggatccagtc ggtcgccgac tcgagcgcgt cgatcgccgc gccctggccg tcgccgagct
     3421 cggcgagccc cgcggccgag agcgccgtgg cgctcgcgcg cagctcgacg atcagctcgc
     3481 gcagcgtggc gccgccctcg cgcaggatct tgcgccccac cagatcgttg gcctggatgc
     3541 cggtggtgcc ctcgtagatg gtgatgatgc gggcgtcgcg gaagtgctgc gcgactccgg
     3601 tctcctcgac gaagcccatg ccgccgaaga cctggatggc ctcgtcgcac acgtcgttgc
     3661 cgagctcggt gcaccagccc ttggccaccg gcatcagcag gtcgacgtag cggcggtgct
     3721 tgtccgccac cgcggcatcg gggtggtggt gggcgatgtc gaaccagccc gcggcggtgt
     3781 agagcaacgc acgcatcgcc atcacccgcg cccgcatcga cagcagcatg cgcttgacgt
     3841 cggggtggca caggatgggc cggcccgcgg ccccgctcac cgcgtcgcgc ccctgcacgc
     3901 gctcgcgggc gtagccgagc gccatctggt aggcgcgctc ggcgagcgcc gcgccctgca
     3961 cgccgacgcc gaagcgcgcc tcgttcatca tcacgaacat gacctcgagg ccgcggttgg
     4021 gctcgcccac cagccagccg gtcgcgccgc cgccctcgcc gaagctgagc gtgcaggtcg
     4081 ggctgccgtg gatgcccatc ttgtgttcga tcgagatgca gcgcacgtcg ttgcgcgcac
     4141 ccaggctgcc atcgggctcg acgaggaact tgggcacgat gaagagcgac agccccttca
     4201 cccccgccgg tgcgtcgggc aggcgggcga gcaccaggtg gacgatgttg ggcgtgagct
     4261 cgtgctcgcc gtaggagatg aagatcttct gcccgaacag cttgtagctg ccgtcggcct
     4321 gcggttcggc gcgggtgcgc gtggcggcga ggtcggagcc cgcctgcggc tcggtcaggt
     4381 tcatcgtgct gccccactcg ccgctcacca ccttgggcag ccaggtgcgc ttctgctccg
     4441 cgctggcggc gatccgcagc gcctcggcct ggccctggtt gaggatgggc agcatggtga
     4501 aggccatgtt ggcggcgttc cacatctcca tcaccggcgt ggacaccagc ttgggcagtc
     4561 cctggccgcc gaactcgacc ggcagcgaca ggccgagcca gcccgcggca cagaactgcg
     4621 cataggcctg ctgccagccc tcgggggtgg cgacgcggcc gccctccagc aagcggcagc
     4681 cctgctcgtc gcccgcgcgg ttcagcggcg ccagcacgcc ggcggcgaag cggccggctt
     4741 cctcgaggac ggcgccgatc acgtcggcgc tggcctcctc gcagccgggc aggcgggcga
     4801 tgtcgtcgag cccggcgagc tcggcgagga tgaactgcat gtcccgcaag gggggctggt
     4861 agtcgtacat ccggagtctc ctctctgtcg ttcttgttct cgcgcgctca gctgcaggcg
     4921 gcgcggagat cctcggggat cggaattgcc ttgatgcgca ggggatcctc cgggtcctgg
     4981 accgcgaaca cgcgcacctc cctgccctcg cacagcacgg tgtcgccgcg ccgcgcgacg
     5041 tgacgcatca cgaagctctt gccgccccag tcctcgaccc aggactcgat ctcgatgtcc
     5101 tcgccatagg tggccgagtt gcggaacttc gcctgcgcgt cgaccagggg cgcgccgatg
     5161 atgccgcgcg tagccgtggt ctcgcgccag ctcggcacgc cgcaggcggt gcagaactcg
     5221 cggaaggcgg tgtcgaacca cttgaagtag ttggcgaaga acacgatctg cgcggggtcg
     5281 cagtcgctga acgcgatgcg catgcggtgg aggtggcggc gtgccatggc gggccccgtc
     5341 tcagcgcgcg gccaggcgca ccgcgccgtc tagccggatc gtctcgccgt tgagcatcac
     5401 gttgtcgatg atgctcagcg ccagcttggc gaactcctcg ggctcgccga ggcgcggcgg
     5461 gaagggcgtg ttctcgccga gcgacttctg cacctcgggc ggcaggccgc gcatcatcgg
     5521 ggtgatgaac aggcccggcg cgatcgtcat cacgcggatg ccgaagcgcg cgagctcgcg
     5581 cgcgatcggc agcgtcatcg acacgatgcc gcccttcgag gccgcgtagg cggcctggcc
     5641 gatctggccc tcgtaggccg cgaccgaggc ggtgttcacg atcacgccgc gctcgccgtc
     5701 ggcctgcggc acccccttgc tcatcgcctc ggcggcgagc cggatcatgt tgaaggtgcc
     5761 gatcaggttg atgcggatcg agcgctcgaa gtcggcgagc ggctgcgcgc cctccttgcc
     5821 gagcaccttg ccggcgttgc cgatgccggc gcagttgacc agcccctgca ggccgccgaa
     5881 gcggctcacc gccaggtcca ccgccgcgcg cgcgtccgcc tcggcggaga cgtcggtgcg
     5941 ggcgaaggcg gcgcgctcgc cgagcgcggt ggcgagcggc tcgccctgcc cggcattgag
     6001 gtcggcgatc accacgttgg cgccggcgcc atgcagcgcg cgtaccgtgg cctcgcccag
     6061 gccggaggcg ccgccggtga cgatgaaggt attgcctgcg aatctcattg cgttctcccg
     6121 gtcgccgctc aggcgacgtt ctccacgatc accgccacgc cctggccgcc gccggcgcag
     6181 gccgaggcca cgccgtactg cagcccggcg gcacgcacgg cgcgcgccac ggtgtgggtc
     6241 aggcgcacgc ccgaggcacc gagcggatga ccgatggcga tcgcgccgcc atgcacgttg
     6301 gccttgtcgc ggtcgagcgc gagctcgcgc tcgcaggcga ggtactgcgc gccgaaggcc
     6361 tcgttgatct cgacgcgacc gatgtcctcg accgcgatgc cggccagccg tgcggcggcg
     6421 cggatcgccg gcgccgggcc gatgcccatg atctcgggcg gcaccgccac cgccgcgccc
     6481 gccaccaccc gggccagcgg ctgcagcccg tgggcgcgca cccagtcgcc gcgcgccacc
     6541 agcaccgccg ccgcgccatc gacgatggcc gagctgttgc cgccggtctg cacgccacca
     6601 aaggcgggct tgagcttctg caggatctcg agcgcggtcg gccgcacgtg gccgtcgcga
     6661 tcgcacacgc tggcgcgatc ggcgaggcgg atgccgcgcg gcttgtagcc gtcgagctcc
     6721 caggtctcgt tcgccagcgc gctcacctcg tctccgaacc agcctgcctc ccaggccgcc
     6781 acgccgcggg cgaagctctg cgcggcgaag cggtcgacct cgtcgcggtc gatgccatag
     6841 cgcaccgcga ggttctcggc ggtgccgccc atcgaggtct tcggtgcggt gtccagcgtg
     6901 gcctcccaga ggaagtcctt gaactcgacc tgccccatgc ggaagcccgc gcggtgggtg
     6961 taggccgcca ccgggttgcg cgacatcgac tcggcgccga cgcagagcac gacgttcgcc
     7021 ttgccgagcg tgatgtggtc ggcggcggtg aggatggtgt cgaagccggt gcagcacagg
     7081 cgctgcacca gcagggccgg cacgtcggga ttcacgcccg agtacagccc gatgtggcgc
     7141 ggcaggtagt aggcgtcgaa gctcgactgc gccatgttgc cggcgacgat cgcgtcgacc
     7201 tcggcggcgg ggatcccgct gtcggcgaac agcgcgcgcg ccgcggcgat gccgaggtcg
     7261 gtcggcgaga cgtcgcgcag cacggtgttg tagtcggcga acggcgtgcg gcggccggcc
     7321 accagccaga tgtcgtcgta ggcgaggccg cgcgcggccg cctccgactc ggcgaagtta
     7381 cccatgccct gcttgctcat tgacgtgctc cctggccctt gcgctggagg aattcgccga
     7441 tgcgtgcggc gatctcgggg ttggtctgga cgaccgcggc ggtcagcgac tcgacgaaga
     7501 agccgtcgtc gctggccatg ttgtcgatgc gggcgatcgc ggtgaccatc gcccagttgg
     7561 ccatcggcgc gttctcggcg acgcggcgcg cgagctcctg cgccttggcg agcgtctcgc
     7621 ccgcgcccac gaggtagtgc gacaggccga ggcgctggcc ctcgtcggcg tcgaggatgc
     7681 ggccggtgag catcatttcg cacatgcgcc cggcgcccac gatcttcgcc acgcgcaccg
     7741 aggcgccccc gccgacgtag atgccgtggc ggccttcggg cagctggtag atcgtgctgg
     7801 gctcggcgac gcggatgtgc gccgcggtgg ccagctctag cccaccgccg atcaccgcgc
     7861 cctgcatcgc ggcgatcacc gggatgccgc cgttctggat gcgggcgaag atgcggtgcc
     7921 aggactgcga gtgcagcatg gtgccgaagg cgtcgcggtg ctggtgctcg gagaggtcca
     7981 accccgcgca gaagtgcttg ccctcgcccg ccaggatgat ggcgcgggtg ccctcggggg
     8041 tggcgaccag cgccgcctcg agcgccgcga gcaggcggtc gctgatcgcg ttgcgcttgt
     8101 ccggacgggc cagcaggagg gtgtagatgc cgtcctcgga cgtggtcttg acgagctgtt
     8161 cggtcattgc ttgttccctt tcgatgaaaa tcacgccgcc ggatggaccg tgatgacggt
     8221 cttgtcgagc acgtcggcgt agaggaactc gaccaggtct gcgcggcggg tcagcaccgc
     8281 gcgctggttg atgtatccct tgtcggtgat ctccccggcc tcgaccgagg gcggctccgc
     8341 catcaggatg gcgcgcgtgg gatgggtgga ggagcccccg cccttcttga tcatcgccgc
     8401 catgccctgc gcgacgcggc tgcggatcgc cgggttgagc agcaggtcga tgatgtcggc
     8461 gtcgggtgcc agatgcgggc acaggcggcg gcattcgggc aggttgggga agatcaggaa
     8521 gccgatctcg tcgcggtcgt ggccggtgac gacgatgtcc tgcgcgatcg gcgacatcgc
     8581 gtcgatcccc gccacccgca ccgagcccac atgcacccag gtgccggtga ggagcttgaa
     8641 gtcctcgccg acgcggccgt cgtagagcag gccgcgctcg gggtgcgccg catcgacgaa
     8701 ctcgacggcg tcgccgatca ggtagtaccc ctcgtcgtcg aaggacttcg cggtcagctc
     8761 gggctgcttc cagtagccgg tgaagacgtt gggccccctg acccgcacct cgagcttgcc
     8821 gtgcgagggc acgagcttga gctcggtgcc gggcaccggc accccgatca cgcccgcgcg
     8881 caccgcctgg aagtggcagt ccgtggccag cggcgcggtc tcggtggagc cccacgagga
     8941 caccatgggg atgcgcgcgc cgacgctcgc ctcggacaac gcctcgagct cgttccagag
     9001 gtgctgcggc agtgccgcac cggcgtacat gatcacctgc aggcggcgga agaagcgctc
     9061 gcgcagggcg gcgtcctggc gcaggtgggg caccagcatg tcgtaggcgc gcggcacgct
     9121 gaaatagatc gtcggcgaca cctcgcgcag gttgcgcagg gtcttctcga tcagcgcggg
     9181 cgtgggcttg ccgtcgtcga tgtagaagct gccgccccag cgcagcacca tgttgaagtc
     9241 gtggttgccg ccgaaggtgt ggctccacgg cagccactcg cacactaccg ggcggttgct
     9301 gcgcaggaag ggccacacca gctccttggc gagctggttc gagcacatca tgcgctgggt
     9361 gttgatgacc gccttcggcg tgcccaccga acccgaggtg aacaggaact tggcgatggt
     9421 gtcggggccg accgcagcga aggccgccat gacggccgcc tcgtcggcct tcgcagcggc
     9481 ctgcgcctcg atctgcgcga tgggcagcac gccgggctgc ggctggctgc gactgccggc
     9541 gacgacgaca ccgtcgtgca ggtcggccac cgccgcgatc gcgggcagga agcgctcgat
     9601 cgggtcggcg aagaccacgc ccgggcgcag cagctcgaag ttgccgcgca gcttgccgaa
     9661 gtccttcgac atcagcgagt tgcccggcga gatcggcgcc accggcacgc cgacgtgcat
     9721 cgccgccagc gccagcaggg catgctcgat gccgttgtcc gagaggatca ccaccggccg
     9781 ctcggccgag agcttctggc cgagcagcca ggaggcgatc gccacgacgc ggcggcgggc
     9841 ctcgccatag ctgagccgat cccattcgcc ggcggcgttg cgctcggcga ggaagacctc
     9901 gtcgggctgc accgcggccc agtgctccag ccagtcgccc acgcagcgcg ggatcgcatc
     9961 cggcagctcg atgcccgaac gcaggatgcg gtcgccgttg ggccggtgcc ggatgatcag
    10021 gtccgggtcg gcgaacaggc gtgcagcgtc gcgaatgatc tcagccatgg cctcgtctcc
    10081 tccttttagc gtcgcggcgg ctcgcactta ctgcgcgacc agcttgtagg cgccgccttc
    10141 gatgcggatc agcacgcggg aacggtcgtc caggccgaag tggtcgttcg gggtcatgtt
    10201 gaacacccca tgcacgccca ccacctcctt gctcgcctcg agcgcatcgc gcagcgcggc
    10261 gcggaactcg gcggtaccgg gcgcgccctt ggcctttgcc gcgggcactg ccgcctcgat
    10321 caggcggaag gcgtcgtggg catagccggc aaaggcggag aagctgcccg cgccgtactg
    10381 ggcctcgtac ttcgtgacga aatcgacacc ggaggccttg gagggatggc tgtcgggaag
    10441 ctgcctgacg accgagaccg gccccaccgg gaggatcgcg ccctcggccg ccttgccggc
    10501 gacaccgagg aaggcctggt tggcggcggc gtgggtctgg tagatctggc ccttgtagcc
    10561 gcgctcgacc agcgcgccct gcggcagcgc ggcggggctg cccgaaccgg cgaccagcac
    10621 ggcatccggg cgcgccgtgg tgagcttgag cacctggccg gtcaccgagg tgtcgttgcg
    10681 gttgtagcgc tcgaccacgg tgagctggat gccggccgcc ccggcgtgct tggtcaccga
    10741 gttcagccag tcctcgccgt aggcgtccga gaagccgatg aagccgagcg tcttgtagcc
    10801 cttggacttc atgtgctcga ccagcgcgct cgccatcacc tcgttctgct gcggcgtgcg
    10861 gaagacccac tcgttgcgct cgaccggcag ggacaccggg ctcatcgtca ccagcggggt
    10921 cttggtctcg tgcgccacct cggccatcgc ggcggtggcc ggggtgctgg acgaaccgat
    10981 gatcgcatcc acgcggtcct cggtgaccag gcggcgggca ttcttggtgg ccacggtggg
    11041 atcggtggcg tcgtccagca cgaccagctt gaccttctcg ccgccgatct cggtcggcag
    11101 cagggcgaag gcgttcttct cggggatgcc gagcgaggcg gccgggccgg tggccgagag
    11161 cgagaccccg atggtgatgt cggcgaacgc agcggagctg agggtgaagg tggcggagag
    11221 cgcgacgacg agcttggcga gtttcatgtt tcctccaatt ggctggtgtg gtgaccgcta
    11281 cggcaagggc cggcggaaag tcggctgctc tctcgcggga ctgcagctct tctaggctgt
    11341 gcgcaagata tcgcttcact aactaccttg tcaaacactt gttcaattac tttttcgatt
    11401 gaccttcggg catcatctac cggaacatcc agccgtcccc cagggccacg aagccgcgcg
    11461 ttcagctcgc ttttcgctgc gatgcggcgg aaaatccgct ccggatgaga gacacgaccc
    11521 gaaacgaaac ccccatgaac gcagcacgac ccgacggcgc cgcaatggcg cccatgaccg
    11581 acacggaggc cgcgatcctc cagctcgcgc gcgaggcgcc cgagctcggc caggtcgcgg
    11641 tggcggaacg gctccgcagc gaaggcctgc ggatctcgcc ctcgggcgtg cgctacctct
    11701 ggcagaagca cggcctcgag actgcggtga agcgcctgca cgcgctggag gaaggcaggg
    11761 gcgcggaggc actgacggag gcgcagcgcc gcctgctcga gcgcggcctg ctcaccgagc
    11821 gcctggcgcg cggcgagcag gcggaggacg gagcgggcaa agagcaggag ccgaccgagc
    11881 gccgccggct gatcctggat gcagctgcac gcctgttctc cgcagagggc tacgaccgca
    11941 cctcgatccg cgacatcgca cgcaacgtcg gcctgctgcc cggctcggtc tatcactact
    12001 tcccgtccaa gcgcgagctc ttcctcgccg ttcatcgcga gggcttcgag cacacgctcg
    12061 agcgcgtccg cgaagcggcc gcgagcggcg gcgatccctg ggaatgcctg cggcgcgcct
    12121 gcgaagccca cgtcgaaggc atcgttgggg gctcctcggt ggatcgcctg gcggggagca
    12181 acctggcgac cgcgcagaac gacgagctgc tcaacgagat ccgtccccat cgtgccgcct
    12241 acgagaccgt gttcaagaca ctgatcgacg ccctgcccct gcgcgcggga accgatcgct
    12301 cgctgctgcg cctgttcctg ctgggcggga tgaactgggt ctatctctgg taccgagagg
    12361 gcaagcgctc gccgcgcgag atcgccgacg cgatggtcga catgctgcgc agcggcgtgc
    12421 agccctgagc gacgcgcgtc cgccgcgcgg cgctcattcc cccagcacgc cgatggcttc
    12481 gtcgtaccag gcgatccagc cctgctcgta ctggatgccg gccttcagga tgaggtggtg
    12541 gatgcgccgc tggcgggtgg gctcgacctc ggcgggaaag tcgcgcgcct cgatggcgcg
    12601 ataggcgcgc agcttgtcgg cgtggagcgc gcgacggcga tcgagttcgg gcagcaggcc
    12661 gagcggcccg gtgaccgcgt cggcgcgcag gcggaccatg aactcgtcgc gcaactcggg
    12721 caggggcgag gcctcggcgg cccagcgcat cagctcgtcg cgcccggtgg gcagcacgct
    12781 gtagctgcgc ttgcgggtgc ggccggcgtc ggggtcggcg tcggagcgga tccaccccgc
    12841 cttctccatg cggccgagct cgcggtagat ctgctgatgc gtggcctgcc agaagtggcc
    12901 gatggacttg tcgaaacgcc gtgcgagctc gtagccggac gagggttgtt cgagcagcga
    12961 ggtgagcagg gcatgggcga gcgacatgca cgaagtctag ccgcgcgcag caggagcatg
    13021 caacgcgttg catagttggc gcgaatcttc tacagtcctg caactggttg catagtcgga
    13081 cggccggtgg gcggcgcagg cctgcccctg ccccgcgcgt ctctccctcc cctctcccgc
    13141 gcgcggggca gcccctcccc tgccgcccgg ccatgcgacc agccccgata aaaatccgcg
    13201 agcgagacga tggccaccta cccgaacctg ttccgtcccc tggacctcgg cttcaccacc
    13261 ctgcccaacc gcttcctgat gggctcgatg catgtcggcc tggaagaggc cgaggggggg
    13321 gtcgagcgca tggccgcgtt ctacgccgag cgggtgcgcg gcggcgtagg gctgatcgtc
    13381 accggcggca tcgcccccaa cctcgagggc cggccgtgga gcggcggcgc cacgctgacg
    13441 acttccgagg aggccgcacg ccatcgcgtg atcaccgacg cggtgcatcg cgagggtgga
    13501 aagatcgccc tgcagatcct gcacttcggc cgctacgcct atcaccccga gctggtcgca
    13561 ccgagcccga tccaggcccc gatcgcgccc ttcgtaccgc gggaactctc cgccgccgac
    13621 gtcgagcgca cgatcgagga cttcgtacgc tgcgccgaac tcgcccgcga aggcggctac
    13681 gacggcgtcg agatcatggg ctccgagggc tacctgatca acgagttcat cgtcgcccat
    13741 accaacaagc gcacggacgc atggggcggc gccttcgaga accgcatccg cttcgccacc
    13801 gagatcgtgc gccgcacgcg cgcgcgcctc gggcgcgagt tcatcctcat cttccgcctg
    13861 tcgctgctcg acctggtcga ggacggctcc accttcgagg aggtggtgca gctcgcgcag
    13921 gcgatcgagg ccgccggcgc cacgctgatc aacagcggca tcggctggca cgaggcgcgc
    13981 atccccacca tcgccacctg cgtgccgcgc gccggcttcg cctgggtgac gcagaaactc
    14041 aaggaccatg tcggcatccc gctgatcgcc accaaccgca tcaacacgcc cgaaatcgcc
    14101 gagagcatcc tcgccgaggg caaggccgac atggtgtcga tggcgcggcc tttcctggcc
    14161 gacccggact tcgtgcgcaa ggctgcggaa ggccgtggcg ccgacatcaa cacctgcatc
    14221 gcctgcaacc aggcctgcct ggaccatacc ttcgccggca agatcacctc ctgcctggtg
    14281 aacccgcgcg cctgccacga gaccgagctg gtgatcgagc cgaccacggc cccgcgcagg
    14341 atcgcggtgg tcggcgccgg cccggcgggc ctggccttcg ccaccaccgc cgccgagcgc
    14401 gggcacaagg tcgtgctgtt cgaggccggg gcgcgcatcg gcgggcagtt caacatcgcg
    14461 atgcagatcc cgggcaagga ggaattcgcc gagaccctgc gctacttcgg gcgccgcatc
    14521 gaacagaccg gggtcgcgct gaagctgaac acccgcgtct cggccgccga gctggccggg
    14581 aagttcgacg aggtcgtgct cgccaccggc atcgtgccgc gcgtgccgga gatcgagggc
    14641 gtggatcatc ccaaggtgct cggctacctc gacgtgctgc gggacagcaa gcccgtcggc
    14701 cgtcgcgtcg ccatcctggg cgcgggcggc atcggcttcg acgtcgcgga gtacctgagc
    14761 cacgagggga tcagccccag cctcgcgccg gcgaagttct acgccgaatg gggcatcgac
    14821 gcgcgctatg cgaaccgcgg cgggctcacc cgcccccatc tcgaaaccgc gccgcgcgag
    14881 atcgtgctgc tgcagcgcaa ggcgagcaag gtcggagagg gcctgggcaa gaccaccggc
    14941 tggatccacc gcaccgcgct gaagaaccgc ggcgtgcgga tgatcgcggg cgtcacctac
    15001 cgccgcatcg atgacgccgg cctgcacgtg agcatcggcg gcaaggacga ggtgctggcg
    15061 gtcgacaacg tgatcctgtg caccggccag gagccgcagc gcgagctgca ggccgcgctg
    15121 gtcgaggccg gcatgcgggt gcatctgatc ggcggcgcgg acgtggccgc cgagctcgac
    15181 gccaagcgcg cgatcaagca gggcatcgag ctggcggcca gcatcgagaa ggcggcatcc
    15241 gcccctgcac tgctcgccgg ccagctcccc gcctctcccg gcggcgccgg catccccctg
    15301 ccgcagttcg acaccctgcg catcggcctc gacggccagg tcgcgctggt gacgctgaac
    15361 cgccccgaca aggccaacgc gatgaacctg cagatgtggc aggacctgcg cgccgcgatg
    15421 cagtgggtgg atcgcacgcc cgcggtgcgc gtggcggtgc tgcacggcgc cggcgccaac
    15481 ttctgcgccg gcatcgacct gcagatgatg atgggcatcc tgcccatggt gaaggacgcc
    15541 tgcgaggcgc gcacgcgcga gaacctgcgc aacctgatcc tcgacctgca ggacacgctc
    15601 accagcctgg agcgctgccg caagccggtg ctcgccgcca tccacggcgc ctgcgtgggc
    15661 ggcggcgtgg acctggtggc ctgcgccgac atgcgctact gcgcggcggg gacgtatttc
    15721 tcggtcaagg aggtcgacct cggcatggtc gccgacgtcg gcagcctgca gcgcctgcca
    15781 cgcctgatcg gcgaaggcat ggtgcgcgag ctcgcctaca ccgggcgccg ggtggacggc
    15841 gcggaggccg ggcggatcgg cctggtgaat cgggtcttcg acacgcccga ggccctgatg
    15901 gagggggtga tgcagctcgc gcaagccatc gccgccaagt cgccgctggc gatccgcggc
    15961 accaaggaca tgctcaacca cgcccgcgac cacagcgtcg ccgacggtct cgaccgcgtc
    16021 gccacctgga acgccgcgat gctgctgtcc gacgacctgc aggcggcgat ccgcgcaggc
    16081 ctcaccaagc agccaccgaa attccgcgac tgaacgcggt ctgagggcat gggtgcgcgt
    16141 gcgttatgtc cgtggcgcgc tgcaggagcg acgcaaatcg cgattgcagc gtctccgccc
    16201 tc
//
LOCUS       DAOQBS010000065        16094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2703_length_16094_cov_15.711703, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000065 DAOQBS010000000
VERSION     DAOQBS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 16094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16094
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2703_length_16094_cov_15.711703"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PKN25_08870"
     CDS             <1..333
                     /locus_tag="PKN25_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HNV91052.1"
                     /translation="AIRRARALAESAWRPALVSPAVTVAGRNGIATCEMMLVNMAEGG
                     FISAHDYRVAKAAATALCGGEVDTNRRVSEQWILDVERAQFVELLKTEKTQQRIVHML
                     ETGKPLRN"
     gene            348..1547
                     /locus_tag="PKN25_08875"
     CDS             348..1547
                     /locus_tag="PKN25_08875"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNV91053.1"
                     /translation="MSKQIQDAYIVAAVRTPVAKRNGAFRAVRPDDLLARVLREVVAK
                     VPSLDRTEIGDVVTGCAMPEAEQGMNVARIGLLLAGLPDSVPGITINRFCASGLQAVA
                     DAASRIRLGEADVMIAAGTESMSAMPQIMGNKVSLNPEIFARAENLSIAYGMGLTAEK
                     VAQQWKVSREDQDAFAVESHRRACAAIAAGQFADEILPYAVKSYAPGVDGTVRITERL
                     VEHDEGPRPDAAADRLGKLKPVFAARGSVTAGNSSQMSDGAAAVLLMSETALKRYDVT
                     PIARFSSFAVAGVPPEVMGIGPVEAIPRALARAGVALADIGWTELNEAFAAQALAVMR
                     QLGMDPAKVNPLGGAIALGHPLGATGAIRTATLMAAMQRDPGLRYGMISMCIGTGMGA
                     AGVFERV"
     gene            1899..3239
                     /locus_tag="PKN25_08880"
     CDS             1899..3239
                     /locus_tag="PKN25_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HNV91054.1"
                     /translation="MIRIKRGLDLPITGAPEQRIEAGRPVRSVAVVGFDYHGMKPTMA
                     VQVGDRVKLGQVLFSDKKTPGVVFTAPGAGTVSAIHRGEQRVLQSVVIDLDGDDAVEF
                     TRYTDAELDRLDDATVRSKLIDSGLWTALRARPFSKVPAIDAKPHSIFVTAIDTHPLA
                     ADPTVVIAEHTDDFARGVRVLARIAPVVVCHADGTRMPCSGLADVRTETFAGPHPAGL
                     AGTHIHFLDPVHAEKSVWSLNYQDAIAIGKLFSTGRLWTERVVSIAGPMAAKPRLVRT
                     RLGANLDELVAGELKTGKKVRVISGSVFGGRTSRGACAFLGRYHLQVSCLEEGSEREM
                     LHYLRAGANKHSVMNLYISKLSPGRLFDFTTSTNGSPRAMVPIGNYEEVMPLDILPTQ
                     LLRALIVGDTDMAQKLGCLELDEEDLALCSYVCAGKYEYGPILRDNLTRIEKEG"
     gene            3242..4456
                     /locus_tag="PKN25_08885"
     CDS             3242..4456
                     /locus_tag="PKN25_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HNV91055.1"
                     /translation="MSLRSWLDSIEHHFEKGGKYEKYYALYEAVDTALYKPPLVTRNT
                     AHVRDGLDLKRMMITVWLCTFPAMFFGMWNVGYQANTILAGSPELMAAQEGWRIALTS
                     ALAGLDPASLWANFLHGATYFLPIYLTTFIVGGFWEVLFASIRKHEVNEGFFVTSVLF
                     ALTLPPDTPLWQVALGISFGVVIAKEVFGGTGKNFLNPALAGRAFLYFAYPAQMSGDA
                     VWTAVDGFTGATMLSLGAAGGIEAVTGAGIDWMRAFLGTVHGSIGETSTLAILIGGAV
                     LLVMKIASWRIVSGVMLGMVATSLLFNAIGSDTNPMFAVPWHWHLVMGGFAFGMMFMA
                     TDPVSASMTNTGKWIFGAVVGIMTVLVRVVNPAFPEGIMLAILFGNLCAPLIDHFVVT
                     ANIKRRLARNVQ"
     gene            4446..5231
                     /locus_tag="PKN25_08890"
     CDS             4446..5231
                     /locus_tag="PKN25_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HNV91056.1"
                     /translation="MSNRESTSRTLMVALAVSLVSSVFVAGAAVSLKPVQVENRLLDK
                     QRSILSIAGLGDAEMSSREVKDLFAGRIKARVVDLQTGEYTDAHDAQLFDPLKAARDP
                     KLSDALDGEEDIALIKRRERFTTVYMVEKDGKLESLILPVRGYGLWSTLHGFVALKGD
                     LNTVVGLGFYQHAETPGLGGEVDNPKWKALWPGKVLFDAQGKPAIHIVKGGVDPASPD
                     AKHQVDGLAGATLTSNGVDRLLQFWLGEQGFGPYLAKLRTQGV"
     gene            5234..5902
                     /locus_tag="PKN25_08895"
     CDS             5234..5902
                     /locus_tag="PKN25_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HNV91057.1"
                     /translation="MSKPTVKEVLLNPILNNNPIALQILGICSALAVTSNLKTAVVMS
                     IALTLVTACSSMFISMIRSQIPSSIRMIVQMVIIASLVIVVDQLLRAYAFSLSKQLSV
                     FVGLIITNCIVMGRAEAFAMQNPPLLSFMDGIGNGLGYSAMLITLGVIRELFGAGKLF
                     GIEIIALAKDGGWYVPNGLLLLPPSAFFLIGLIIWGLRTWKKEQVEKPAFRMAPQVVE
                     KEAY"
     gene            5904..6512
                     /gene="nqrE"
                     /locus_tag="PKN25_08900"
     CDS             5904..6512
                     /gene="nqrE"
                     /locus_tag="PKN25_08900"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HNV91058.1"
                     /translation="MEHLISLFVRSVFIENMALSFFLGMCSFIAISKKVETAIGLGVA
                     VVVVQAITVPANNLMYNLLLREGALAWAGLPEVDLSFLGLLSYIGVIAAIVQILEMLL
                     DKYMPSLYNALGIFLPLITVNCVIMAGSLFMVERDYTLAESAVYGVGSGASWALAIAL
                     LAGIREKLKYSDVPEGLQGLGITFITIGLMSLGFMSFSGVQL"
     gene            6528..7751
                     /gene="nqrF"
                     /locus_tag="PKN25_08905"
     CDS             6528..7751
                     /gene="nqrF"
                     /locus_tag="PKN25_08905"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HNV91059.1"
                     /translation="MNTEIILGIGMFTAIVLALAVFIIAARSKLVSSGDVTIDINGEK
                     KIVVPAGGKLLQTLAGNGLFLPSACGGGGTCAQCKCVVMEGGGSMLPTEESHFTKRDA
                     KEGWRLSCQTPVKQDMKIEVPEEVFGVKKWECTVESNPNVATFIKELTLRLPEGEDVH
                     FRAGGYVQLECPPHVVNYKDFDIQDEYRGDWDKFNLWRFVSKVDETTIRAYSMANYPD
                     EKGVVKFNIRVASPPPGRDDVPPGKMSSWVFNLKKGDKVTVYGPFGEFFARDTDHEMV
                     FVGGGAGMAPMRSHIFDQLKRLHSKRKISFWYGARSMREAFYVEEFDKLAAENPNFKW
                     HLALSDPLPEDNWTGYTGFIHNVLYENYLKDHPAPEDCEFYMCGPPMMNAAVIKLLLD
                     LGVERENIMLDDFGG"
     gene            complement(7893..8672)
                     /locus_tag="PKN25_08910"
     CDS             complement(7893..8672)
                     /locus_tag="PKN25_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNV91060.1"
                     /translation="MELDPDTLLFWLKKLVAVLALPPVLPLLPIVIGLITLRRRPRLG
                     LALAWAGVALNLLLIFPPSVGWLVARVEYPQMLDRARAREAQAIVVLGAGKRTHAPEF
                     GGETVNRLALERLRYAAALARETRLPVLVSGGAPAGDTPEAYLMEALLRDEYGVPPRW
                     VESASRDTRENARFSAVHLQAAGVKRILLVTHAMHMERARPEFEAAGLDVIPAPTAWL
                     GGGDSPGDAQPLPFAPSQNTAYAAWFALHELLGRLAYRWSR"
     gene            8837..9445
                     /locus_tag="PKN25_08915"
     CDS             8837..9445
                     /locus_tag="PKN25_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HNV91061.1"
                     /translation="MREALWSLALLLAAYAAFEFLRALRLPREGASGLAVGPQPVTGT
                     RTAAAAAAHDARDDEDDDFLAYAPLPSSVSSAAPAGAAPSAPTSAPAASSGAAEAEAA
                     AAARFELELENSRLRQALQAQRDLVALQQTEIDRLVEDIAALRDELDAQASSVASSPE
                     YSEAMVLARQGLNAEMIAVRCGITLAEAELVLALGRGAEGHG"
     gene            9442..10107
                     /locus_tag="PKN25_08920"
     CDS             9442..10107
                     /locus_tag="PKN25_08920"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNV91062.1"
                     /translation="MKLPRFPATRSALPRGSPRRAALLRAAAALAAALVLLVLGLLDR
                     DRPVPSRAGAGAAVGGAAPGAAASTPPAMPANPLSAAGKPGPGAPAYEAAVPLTAGAG
                     QGAACAPPSAPPPSCPTPPPAPACTPLPVPTAKLDGVLLPRAAVAPAGSGYLVQLGVF
                     AAPDNALRIYQQAAAAGQPAHIQSRVVLGPFVDREAAERARKRLQAAGAGPGVLIPPE
                     RPR"
     gene            10182..10619
                     /gene="fliW"
                     /locus_tag="PKN25_08925"
     CDS             10182..10619
                     /gene="fliW"
                     /locus_tag="PKN25_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNV91063.1"
                     /translation="MQIKSPLFGSAEIAEDKIIEFPKGLPGFEECRRFTLLHEEGREA
                     HVFLLQSVDEPDVAFSVTAPERLGVHLEFPLSDEEVAMLRLRTPEDAAVAVIVRKDEG
                     ESPAGTGLRANFMAPLVINTGERVGLQKLIDRLGCEITLRAGG"
     gene            10701..11081
                     /locus_tag="PKN25_08930"
     CDS             10701..11081
                     /locus_tag="PKN25_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNV91064.1"
                     /translation="MARQIISTPKAPAAIGPYSQAVKVGNQVFLSGQIGLDPVTMEMV
                     EGVEAQARRVFDNLAAVAEAAGGSFADVVKITIYLTDLANFGVVNEIMKSCFAEPYPA
                     RATVGVRELPRGALVEADLVLVLG"
     gene            11176..13299
                     /gene="recG"
                     /locus_tag="PKN25_08935"
     CDS             11176..13299
                     /gene="recG"
                     /locus_tag="PKN25_08935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNV91065.1"
                     /translation="MPADQRRARETCCPFPPSEKDRRLNATQPPRGWPGVGAQLAGRL
                     ARLDLRGPRDVLLHLPLRYEDETRLTPIARLRPGFPAQVEGEVLGSEVVLRPRRQLVA
                     RIADDSGTLVARWLNFYPSQQKPLAPGGRVRLFGEARGGFFGLEMVHPRLRAVAPGEA
                     LPDALTPVYPTTAGVGQITLRKLVAQALQSEPLDELLPAAWLHRLGLPGFAEALHALH
                     HPAPDADPFALEQREHPAWQRMKFEELLVQQLSLRRAWLARRAQAAVALPARALLTAA
                     LLESLPFRPTGAQARVGAEIAADLAAPHPMQRLLQGDVGSGKTLVAALAMLQAAENGW
                     QAAMMAPTEILAEQHWKKLSAWLEPLGIGVAWLSGSRRKRAREAELARLASGEVLLAV
                     GTHALIEDPVTLPRLALVVVDEQHRFGVRQRLALREKGEGGRRPHMLMMSATPIPRTL
                     AMTHYADLDVSVLDELPPGRTPIRTRLVSDVRRDEVVARVRAACLEGRQAYWVCPLIE
                     ESEALQLQTAQDTFATLVEALPELRVGLVHGRLRNEEKSATMAAFSAGALDLLVATTV
                     IEVGVDVPNASLMVIEHAERFGLAQLHQLRGRVGRGTAESVCILIYAQPLSQTGRERL
                     KVIYENTDGFAIAREDLRIRGPGEFVGARQSGVPLLRYADLELDAGLVEPARELAEHL
                     LAHDPAAANALMRRWLGGREALLRA"
     gene            13577..14170
                     /locus_tag="PKN25_08940"
     CDS             13577..14170
                     /locus_tag="PKN25_08940"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HNV91066.1"
                     /translation="MRPLTAFPLHTGPRHETWLAHPPRVLLPPALLPWLKDAGSLTAR
                     IRARCTRFEVRVLCQRLAHVRRDEAWLLGLRVGELAWLREVLLVADGRPVVFARSLLP
                     RRNLRGAWNLFHGIGARPLGAALFSDPAIARLPLACRRLDGRDARYHRAAAALARQSR
                     APALPRALWARRSVFLLRGRALMVSEAFLPAILELRK"
     gene            14167..15045
                     /gene="ubiA"
                     /locus_tag="PKN25_08945"
     CDS             14167..15045
                     /gene="ubiA"
                     /locus_tag="PKN25_08945"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNV91067.1"
                     /translation="MTPAMSLSDKLPHYARLMRIDKPIGTLLLLWPTLWALWIAAAGW
                     PPLHILVIFIVGTFLMRSAGCVINDYADRDFDGHVERTRTRPLATGVVSPGEALALAA
                     ALSAAAFVLVLPLNTLTILLSVPALLLAGSYPFTKRFFAIPQAYLGIAFGFGIPMAFA
                     AVTGTVPATGWLMLLANIFWAVAYDTEYAMVDRPDDLKIGIKTSAITFGRHDVLAVML
                     CYAATFVLLTVVGALAGLGFAWYAGLVVACAIAGYHYTLIRARDRADCFKAFRHNNWV
                     GGAIFAGLFIDLLLRA"
     gene            15093..15917
                     /gene="pyrF"
                     /locus_tag="PKN25_08950"
     CDS             15093..15917
                     /gene="pyrF"
                     /locus_tag="PKN25_08950"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNV91068.1"
                     /translation="MHFMTALRAAWQQRDSLLCVGLDPDPARFPAHLQGRSDAILRFC
                     TQIVDATADLVCCFKPQIAYFAAHRAEDQLEALVEHIHARHPGTPVVLDAKRGDIGST
                     AEQYAIEAFERFKADAITVNPYMGRDSVDPYLAYPDKGVILLCRTSNPGGSDLQFLEV
                     DTSKGRMKLYEHVARTVAEDWNASGNCALVVGATFPAEIARVRALVGELPLLVPGIGA
                     QGGDIAATLAAGRTENGTGLMINSSRAVLYAGKGEDFAEAARRVAEETRDAINAYR"
BASE COUNT         2367 a         5776 c         5366 g         2585 t
ORIGIN      
        1 gcgatccgcc gcgcgcgcgc gctggccgag tcggcctggc ggccggcgct ggtcagtccg
       61 gcggtcaccg tggccggacg taacggcatc gccacctgcg aaatgatgct ggtgaacatg
      121 gccgagggcg gtttcatctc ggcgcacgac tatcgcgtgg cgaaggctgc ggccaccgcg
      181 ctgtgcggcg gcgaggtcga caccaaccgg cgcgtgagcg agcagtggat cctcgacgtc
      241 gagcgcgcgc agttcgtcga gctgctcaag accgagaaga cccagcagcg catcgtgcac
      301 atgctggaaa ccggcaagcc gctgcgcaat tgagggagaa acagacaatg agcaaacaga
      361 tccaggacgc ctacatcgtc gccgcggtgc gcactcccgt cgccaagcgc aacggcgctt
      421 tccgcgccgt gcgccccgac gacctgctgg ccagggtgct gcgcgaggtg gtcgccaagg
      481 tgcccagcct agacaggacc gagatcggcg acgtcgtcac cggctgcgcg atgcccgagg
      541 ccgagcaggg catgaacgtg gcacgcatcg gcctgctgct ggccgggctg cccgacagcg
      601 tgccgggcat caccatcaac cgcttctgcg cgtccggcct gcaggccgtg gccgatgccg
      661 ccagccgcat ccgcctcggc gaggcggacg tgatgatcgc cgccggcacc gagagcatga
      721 gcgcgatgcc gcagatcatg ggcaacaagg tcagcctcaa ccccgagatc ttcgcgcgcg
      781 ccgagaacct ctccatcgcc tacggcatgg gcctcaccgc cgagaaggtc gcgcagcagt
      841 ggaaggtgag ccgcgaggac caggatgcgt tcgccgtcga gtcgcaccgg cgcgcctgcg
      901 ccgcgatcgc cgccggccag ttcgcggacg agatcctgcc ctatgcggtg aagtcctatg
      961 ccccgggtgt cgacggcacg gtgcgcatca ccgagcgcct ggtcgagcac gacgagggcc
     1021 cgcgtccgga cgccgctgcc gacaggctcg gcaagctcaa gccggtgttc gccgcgcgcg
     1081 gctcggtgac cgcgggcaac agctcgcaga tgtccgacgg cgccgcggcg gtgctgctga
     1141 tgagcgagac cgcgttaaag cgctacgacg tgaccccgat cgcgcgcttt tccagcttcg
     1201 cggtcgccgg cgtgccgccc gaggtcatgg ggatcggccc cgtcgaggcc atcccgcgtg
     1261 cgctcgcgcg tgccggcgtg gcgctggccg acatcggctg gaccgagctc aacgaggcct
     1321 tcgccgcgca ggcgctggcg gtgatgcgcc agctcgggat ggatccggcg aaggtcaacc
     1381 cgctcggcgg cgcgatcgcc ctcgggcacc cgctcggtgc caccggcgcg atccgcaccg
     1441 ccaccctgat ggcggcgatg cagcgcgacc ccggcctgcg ctacggcatg atcagcatgt
     1501 gcatcggcac cggcatgggt gcggcggggg tgttcgagcg cgtgtgagcg tgaccggcgc
     1561 acgggaagtc catccccgac ttcccttgcg ccgagcttct accatgctga gcgtgagctg
     1621 tcgttttgga gggcgcctcg cgtgcttcgg gagcgcatcc catttgcagc attctcatgc
     1681 gaacggaatt tcttggccgg ttgatggcgc gctcggcgta ttgtggattt cacctagttc
     1741 gctgccttcg ggtattcccc ggcgtgatcg tctggtaaca atcttttata atccgcgcgt
     1801 ttatcgacgc gggaccgcct ccggcgtccg gacgcctggc gctcccggat gcgttgacgg
     1861 cgcccgtgcc tccagtggaa tcaacgggcg agaagcgcat gatcagaatc aaacgcggtc
     1921 tggacctgcc catcaccggc gcgcccgagc agcgcatcga ggccggcagg ccggtgcgca
     1981 gcgtggccgt cgtcggcttc gactatcacg gcatgaaacc gaccatggcg gtgcaggtcg
     2041 gtgaccgggt gaagctcggc caggtcctgt tctccgacaa gaaaacgccc ggtgtggtct
     2101 tcaccgcgcc gggtgccggc accgtcagcg ccatccatcg cggcgagcag cgcgtgctgc
     2161 agtcggtggt gatcgacctc gacggcgatg atgcggtcga attcacgcgc tacacggacg
     2221 ccgaactcga caggctcgac gacgcgacgg tgcgcagcaa gctgatcgac tccggactgt
     2281 ggaccgcact acgcgcgcgg cccttcagca aggtgccggc gatcgacgcg aagccgcact
     2341 cgatcttcgt caccgcgatc gacacccatc cgctcgccgc cgacccgacc gtagtgatcg
     2401 ccgagcacac ggacgacttc gctcgcggcg tgcgtgtgct cgcgcgcatc gcgccggtcg
     2461 tcgtgtgcca cgccgatggg acccggatgc cctgttccgg gctggccgac gtgcgcaccg
     2521 agaccttcgc cggcccgcat ccggccggcc tcgccggcac gcacatccat ttcctcgatc
     2581 cggtgcatgc cgagaagtcg gtgtggtcgc tcaactacca ggacgcgatc gcgatcggca
     2641 agctcttctc caccggccgc ctgtggaccg agcgcgtggt gtcgatcgcc ggccccatgg
     2701 ccgcgaagcc gcgcctcgtg cgcacccgcc tcggcgccaa cctcgacgag ctcgtcgccg
     2761 gcgaactgaa gaccggcaag aaggtgcgcg tgatctccgg ctcggtgttc ggcgggcgga
     2821 cctcgcgcgg agcctgcgcc ttcctcgggc gctaccacct gcaggtgtcc tgcctggaag
     2881 aaggctccga gcgcgagatg ctgcactacc tgcgcgccgg ggcgaacaag cactcggtga
     2941 tgaacctcta catctccaag ctctcgccgg gcaggctgtt cgacttcacc acctcgacca
     3001 acggctcgcc gcgcgcgatg gtgccgatcg ggaactacga agaggtgatg ccgctcgaca
     3061 tcctgcccac gcaattgctg cgcgcgctca tcgtgggcga caccgacatg gcgcagaagc
     3121 tcggctgtct cgagctggac gaggaagacc tcgcgctgtg ttcctacgtg tgcgcgggca
     3181 agtacgaata cggtcccatc ctgcgggaca acctgacccg catcgagaag gagggttgag
     3241 catgagtctg cgctcctggc tcgacagcat cgagcatcat ttcgagaagg gcggcaagta
     3301 cgagaagtac tacgccttgt acgaggccgt ggacaccgcg ctctacaagc cgcccctggt
     3361 cacccgcaac accgcccacg tgcgcgacgg tctcgacctc aagcgcatga tgatcacggt
     3421 gtggctgtgc accttcccgg ccatgttctt cggcatgtgg aacgtcggct accaggccaa
     3481 caccatcctc gccggcagcc ccgagctgat ggccgcgcag gaaggctggc gcatcgcgct
     3541 caccagcgcg ctggccgggc tcgatcccgc gagcctgtgg gccaacttcc tgcacggggc
     3601 gacctacttc ctgccgatct acctgacgac cttcatcgtc ggcggcttct gggaggtgct
     3661 gttcgcctcg atccgcaagc atgaggtcaa cgagggcttc ttcgtcacct cggtgctgtt
     3721 cgcgctcacc ctgccgcccg acaccccgct ctggcaggtg gcgctgggca tcagtttcgg
     3781 tgtggtgatc gcgaaggaag tctttggcgg caccggcaag aacttcctca acccggcgct
     3841 cgccggccgc gccttcctgt atttcgccta cccggcgcag atgtcgggcg acgcggtgtg
     3901 gaccgcggtc gacggcttca ccggcgccac catgttgtcg ctcggcgccg ccggcggcat
     3961 cgaggcggtg accggcgcgg gcatcgactg gatgcgcgct ttcctcggta cggtgcacgg
     4021 ctcgatcggc gagaccagca cgctcgccat cctgatcggc ggtgcggtgc tgctggtgat
     4081 gaagatcgcc tcgtggcgca tcgtctccgg cgtcatgctg ggcatggtcg ccaccagcct
     4141 gctgttcaac gcgatcggct ccgacaccaa cccgatgttc gccgtgccct ggcactggca
     4201 cctggtgatg ggcggcttcg ccttcggcat gatgttcatg gccaccgatc cggtgtcggc
     4261 ctcgatgacc aacaccggca aatggatctt cggtgcggtg gtggggatca tgaccgtgct
     4321 ggtgcgcgtg gtgaaccccg ccttccccga aggcatcatg ctcgccatcc tgttcggcaa
     4381 cctttgtgcg ccgctgatcg accacttcgt ggtcacggcc aacatcaagc ggaggcttgc
     4441 gcgcaatgtc caatagagaa tccacaagcc gaacgctgat ggtggcgctc gcggtcagtc
     4501 tggtgagctc ggtcttcgtc gccggcgcgg cggtgtcgct gaagccggtg caggtggaaa
     4561 accgcctgct cgacaagcag cgcagcatcc tttcgattgc cggcctgggc gatgccgaga
     4621 tgtcttcgcg cgaggtcaag gatctcttcg ccgggcgcat caaggcgcgc gtggtcgatc
     4681 tgcagaccgg cgaatacacc gacgcccacg atgcccagct tttcgacccg ctcaaggctg
     4741 cgcgcgatcc caagctctcc gacgccctcg acggcgagga agacatcgcg ctgatcaagc
     4801 gccgcgagcg cttcaccacg gtctacatgg tggagaagga cggcaagctc gaatcgctga
     4861 tcctgccggt gcgcggctac ggcctgtggt ccacgctgca tggcttcgtc gccttgaagg
     4921 gcgacctcaa caccgtggtc ggcctcggct tctaccagca cgccgagaca ccgggcctcg
     4981 gcggcgaggt cgacaacccg aagtggaagg cgctgtggcc cggcaaggtg ctgttcgacg
     5041 cgcaaggcaa gcccgcgatc cacatcgtca agggcggggt cgatccggcc agccccgacg
     5101 cgaagcacca ggtcgacggc ctcgccggtg ccacgctgac cagcaacggc gtcgaccggc
     5161 tgctccagtt ctggctcggc gaacagggct ttggccccta tctcgccaaa ctccgcaccc
     5221 agggggtctg atcatgtcca agcccaccgt caaggaagtc ctcctcaacc ccatcctcaa
     5281 caacaacccg atcgcgctgc agatcctcgg catctgctcg gcgctcgcgg tcacctccaa
     5341 cctcaagacc gcggtcgtga tgtcgatcgc gctgaccctg gtgacggcgt gctcgagcat
     5401 gttcatctcg atgatccgca gccagatccc gagctcgatc cgcatgatcg tgcagatggt
     5461 gatcatcgcc tcgctggtca tcgtggtgga ccagctcctg cgcgcctacg ccttcagcct
     5521 gtccaagcag ctttcggtgt tcgtcggcct gatcatcacc aactgcatcg tgatgggccg
     5581 tgcggaagcc ttcgcgatgc agaacccgcc gctgctgtcc ttcatggacg gcatcggcaa
     5641 cggtctcggc tacagcgcga tgctgatcac gctcggcgtg atccgcgagc tcttcggtgc
     5701 cggcaagctg ttcggcatcg agatcatcgc gctcgccaag gacggcggct ggtacgtgcc
     5761 caacgggctg ctgctgctgc cgccctcggc cttcttcctg atcggcctga tcatctgggg
     5821 cctgcgcacc tggaagaagg agcaggtcga gaagcctgcc ttccggatgg cgccgcaggt
     5881 cgtggaaaag gaggcctact gaaatggaac acctcatcag cctgttcgtg cgctccgtgt
     5941 tcatcgagaa catggcgctg tccttcttcc tcggcatgtg ctcgttcatc gcgatctcca
     6001 agaaggtcga gaccgcgatc ggcctgggcg tggcggtcgt cgtcgtgcag gcgatcaccg
     6061 tgccggccaa caacctgatg tacaacctgc tgctgcgcga aggcgcgctg gcgtgggcgg
     6121 ggctgccgga ggtcgacctg tccttcctcg gcctgctgtc ctacatcggc gtgatcgcgg
     6181 cgatcgtgca gatcctcgag atgctgctcg acaagtacat gcccagcctc tacaacgcgc
     6241 tcggcatctt cctgccgctg atcacggtca actgcgtgat catggccggc tcgctgttca
     6301 tggtcgaacg cgactacacg cttgctgaaa gcgcggtgta tggcgtgggc tcgggcgcat
     6361 cctgggcgct ggcgatcgcg ctgctcgccg gcatccgcga gaagctcaag tacagcgacg
     6421 tgcccgaggg cctgcagggc ctgggcatca ccttcatcac catcggcctg atgtcgctcg
     6481 gcttcatgtc gttctcgggc gtgcagctct aaggagaccg tgcaacgatg aataccgaaa
     6541 tcatcctcgg catcgggatg ttcaccgcca tcgtgctcgc actggccgtg ttcatcatcg
     6601 ctgcccgctc caagctggtt tcgtcgggcg acgtcaccat cgacatcaac ggcgagaaga
     6661 agatcgtcgt ccccgccggc ggcaagctgc tgcagacgct cgccggcaac ggtctcttcc
     6721 tgccctcggc ctgcggcggc ggcggcacct gcgcgcagtg caagtgcgtg gtcatggaag
     6781 gcggcggctc gatgctgccc acggaggagt cccacttcac caagcgcgac gccaaggaag
     6841 gctggcgtct gtcctgccag accccggtca agcaggacat gaagatcgag gtcccggaag
     6901 aggtcttcgg cgtcaagaag tgggagtgca ccgtcgagtc caaccccaac gtcgccacct
     6961 tcatcaagga gctcaccctg cgcctgcccg aaggggagga cgtgcacttc cgcgccggcg
     7021 gctacgtgca gctcgagtgc ccgccccacg tggtcaatta caaggacttc gacatccagg
     7081 acgagtaccg cggcgactgg gacaagttca acctgtggcg cttcgtctcc aaggtcgacg
     7141 agaccacgat ccgcgcctac tcgatggcga actaccccga cgaaaagggt gtggtgaagt
     7201 tcaacatccg cgtggcttcg cccccgccgg ggcgcgacga tgtcccgccc ggcaagatgt
     7261 cgtcctgggt gttcaacctc aagaagggcg acaaggtcac ggtgtacggt cccttcggcg
     7321 agtttttcgc gcgcgacacc gaccacgaga tggtcttcgt cggcggcggc gcgggcatgg
     7381 cgccgatgcg ctcgcacatc ttcgaccagc tcaagcgtct gcacagcaag cgcaagatca
     7441 gcttctggta cggcgcgcgc tcgatgcgcg aggccttcta cgtcgaggag tttgacaagc
     7501 tcgctgccga gaaccccaac ttcaagtggc acctcgcgct ctccgacccg ctgcccgagg
     7561 ataactggac cggctacacc ggcttcatcc acaacgtgct gtacgagaac tacctcaagg
     7621 accacccggc ccccgaagac tgcgagttct acatgtgcgg tcccccgatg atgaacgccg
     7681 cggtgatcaa gctgctgctc gacctcggtg tcgagcgcga gaacatcatg ctcgacgact
     7741 tcggcggctg aaggctgccg catccgcgac cgcacccgcc cgcgcatccg ccttcagaga
     7801 cgggcgcggg ttttttcatg aacggcgaga cggtctgccg gcgccctgcg gtacgacggg
     7861 gcggtcgtcg tgttctgacc tccccgcagg tctcagcgcg accagcggta ggcgagccgg
     7921 cccagcagct cgtgcagcgc gaaccaggcc gcgtaggcgg tgttctggct tggcgcgaaa
     7981 ggcaggggct gggcgtcgcc ggggctgtcg ccgccgccca gccaggccgt cggagccggg
     8041 atcacgtcga gtccggcggc ctcgaactcg gggcgcgcgc gctccatgtg catcgcgtgg
     8101 gtcaccagca ggatccgctt cacccccgca gcctgcagat gaaccgccga gaaacgtgcg
     8161 ttctcgcgcg tgtcgcgcga ggccgattcc acccagcgcg gcggcacgcc gtattcgtcg
     8221 cgcagcaagg cctccatcag gtaggcctcg ggggtgtcgc cggccggtgc gccgccgctc
     8281 accagcaccg gcagccgggt ctcgcgtgcg agcgcggcag cgtagcgcag gcgctcgagc
     8341 gcgagccggt tgaccgtctc gccgccgaac tcgggcgcgt gcgtgcgctt gccggcgccg
     8401 agcaccacga tcgcctgcgc ctcgcgcgcc cgcgcgcgat cgagcatctg cggatattcg
     8461 acgcgcgcca ccagccaccc cacgctcggc gggaagatca gcagcaggtt cagcgccacg
     8521 cccgcccacg cgagcgccag gccgagccga ggccgccggc gcagggtgat caggccgatc
     8581 acgatcggca gcagcggcag caccggtggc agcgcgagca ctgcgaccag cttcttgagc
     8641 cagaagagca gggtgtcggg gtcgagttcc atggtcggtc ggggcgggtt tggcggcggc
     8701 tgccgcggcc aagtattatc ggtgcatcga gcctctgttg ccctcatgcg ccctctcctg
     8761 aactcccgcc gtcgcccgct gcgagatcgc tgccgattcc gcgtggatga ccgtgcctcg
     8821 tggaggatcg ccgctcatgc gtgaggcctt gtggtcgctg gccctgttgc tggcggccta
     8881 tgccgcgttc gagttcctgc gcgcgctgcg cctgccgcgc gaaggggcgt ccgggcttgc
     8941 cgtcgggccg cagcccgtca cggggacgcg gacggcggcg gccgcggcag cccacgacgc
     9001 gagagatgac gaggatgacg acttcctcgc ctacgcaccc ttgccttcct ccgtttcctc
     9061 cgcggcgcct gcaggcgctg cgccttccgc ccccacctcc gctcctgccg cctcttccgg
     9121 cgctgccgag gccgaggcgg ccgccgcagc gcgcttcgag ctcgagcttg agaactcccg
     9181 gctgcgccag gcactgcagg cgcagcggga cctggtggcg ctgcagcaga ccgagatcga
     9241 tcgtctggtg gaggacatcg ccgcgttgcg cgacgaactg gacgcccagg cgtcgtccgt
     9301 cgccagctcg cccgaatact ccgaagccat ggtgctcgcc aggcaggggc ttaacgccga
     9361 gatgatcgcc gtgcgctgcg gcatcaccct cgccgaggcc gagctggtcc tcgcactcgg
     9421 gcgcggtgcg gaagggcacg gatgaagctg ccgcgctttc cggccacccg ctccgcgctg
     9481 ccgcgcggct cgccgcggcg cgcagcgctg ctccgcgcag ccgcggcgct ggccgccgct
     9541 ctcgtgctgt tggtgctcgg gctgctcgac cgcgaccgtc cggtgccgtc gcgcgcgggc
     9601 gctggcgcgg cggttggtgg ggcggcgccc ggcgctgcgg cctcgactcc tcctgccatg
     9661 cccgcgaacc cgctgtcggc ggccggaaag cccggccccg gtgcgccagc gtacgaggcc
     9721 gccgtgccgc tcacggccgg ggccgggcag ggggctgcat gcgcgccccc atccgcgccc
     9781 ccaccctcct gcccgactcc gccacctgcg ccggcatgca ctccgcttcc ggttcccacc
     9841 gcaaagctcg atggtgtcct gcttccccgt gccgccgtgg cgcccgccgg ctccggctat
     9901 ctcgtccagc tcggcgtctt cgccgccccc gacaacgcgc tgcgcatcta ccagcaggcc
     9961 gcagccgcgg ggcagccggc gcacatccag agccgggtgg tgctcggtcc tttcgtcgac
    10021 cgcgaggccg ccgagcgtgc gcgcaagcgg ctgcaggcgg cgggtgccgg gcctggcgtg
    10081 ttgatccccc cagaacgccc gcgttgagcg gacgtgaagc gtgtcacggc gggtccgccg
    10141 tgcctcggat gatgatgttt ttttgaagcg aggttccagt catgcagatc aagagtccgc
    10201 tgttcggcag cgccgagatt gccgaagaca agatcatcga gtttcccaag ggcctgcccg
    10261 gcttcgagga gtgccgccgc ttcaccctgc tgcacgagga aggacgcgag gcgcatgtgt
    10321 tcctgctgca gagcgtggac gagcccgatg tcgccttctc ggtgaccgcg cccgagcgcc
    10381 tcggcgttca cctggaattc ccgctgagcg acgaggaagt cgcgatgctg cgcctgcgca
    10441 cgcccgagga tgccgcggtc gcggtgatcg tgcgcaagga cgaaggcgaa agcccggccg
    10501 gcaccggcct gcgcgccaat ttcatggcgc cgctggtcat caacaccggc gagcgtgtcg
    10561 gcttgcagaa actgatcgac cgcctcggct gcgagatcac cctgcgcgcc ggcggctgag
    10621 ccgcccgggt gccctgcggg cggcatgtcc gcccgcgccc gcgggtacaa tgccggcttt
    10681 ccccaagcag aggcaaaacc atggcccgtc agatcatctc cacccccaag gcacccgccg
    10741 ccatcggccc ctattcgcag gcggtaaagg tcggcaacca ggtcttcctc tccggccaga
    10801 tcggtctcga ccccgtcacc atggaaatgg tcgagggcgt cgaggcgcag gcgcgtcgcg
    10861 tgttcgacaa cctcgccgcg gtggccgagg cggccggcgg ctccttcgcc gacgtggtca
    10921 agatcaccat ctatctcacc gacctggcca acttcggcgt ggtcaacgag atcatgaagt
    10981 cctgcttcgc cgagccctat ccggcacgtg ccaccgtcgg cgtgcgcgag ctgccgcgcg
    11041 gcgcgctggt cgaggcggat ctggtgctcg tgctcggctg aggcctgcaa ggggcaaggc
    11101 acgaggtgtg aaacttaagg ggcggggcgc tcggagcgct gcctccttgg cataccgctt
    11161 cggtgtcccg gtcgcgtgcc ggccgatcaa cggcgggcgc gcgagacctg ttgccccttt
    11221 cctccttccg agaaggatcg acgattgaac gcgacgcagc cgccgcgcgg ctggccgggg
    11281 gtcggggcgc agctggcggg gcggctggcc aggctcgacc tgcgcgggcc acgcgatgtc
    11341 ctgctccacc tgccgctgcg ctacgaggac gagacccggc tgaccccgat tgcccggttg
    11401 cgccccggct ttcccgccca ggtcgagggc gaggtgctcg gtagcgaggt ggtgctgcgt
    11461 ccgcgccgcc agctggtggc gcggatcgcc gacgacagcg gcaccctggt ggcgcgctgg
    11521 ctcaacttct acccctcgca gcagaaaccg ctcgcgccgg gcgggcgcgt gcgcctgttc
    11581 ggcgaggcgc gcggcggctt cttcgggctc gagatggtgc atccgcgcct gcgcgccgtg
    11641 gcgcccggcg aggcgctgcc cgatgcgctc accccggtct atcccaccac cgccggcgtc
    11701 ggccagatca ccctgcgcaa gctggtcgca caggcgctgc agagcgagcc gctcgacgaa
    11761 ctgctgccgg ccgcctggct gcaccgcctg gggctgcccg gcttcgccga ggccttgcat
    11821 gcgctgcacc atcccgcgcc cgacgccgac cccttcgcgc tcgagcagcg cgagcatccc
    11881 gcctggcagc gcatgaagtt cgaggagctg ctggtgcagc agctctcgct gcgccgcgcc
    11941 tggctcgcgc gccgtgccca ggcggcggtg gcgctgccgg cgcgcgccct gctgaccgcg
    12001 gctttgctcg aatcgctgcc tttccgtccc acgggggcgc aggcgcgggt gggcgcggag
    12061 atcgccgccg acctcgcggc gccgcatccg atgcagcgcc tgctgcaggg cgatgtcggc
    12121 tcgggcaaga ccctggtcgc cgcgctggcg atgctgcagg cggccgagaa cgggtggcag
    12181 gcggcgatga tggcgcccac cgagatcctc gcagagcagc actggaagaa gctctcggcc
    12241 tggctggagc ccctgggcat cggcgtcgcc tggctgtcgg gcagccgcag gaagcgcgcg
    12301 cgcgaggccg agctcgcccg cctggcgagc ggcgaggtgc tgctcgcggt cggcacccat
    12361 gcgctcatcg aggacccggt gacgctgccg cggctggcgc tcgtggtggt cgacgagcag
    12421 caccgcttcg gcgtgcgcca gcgcctggcg ctgcgcgaga agggcgaggg cgggcggcgg
    12481 ccgcacatgc tgatgatgtc ggcgacgccg atcccgcgca cgctggcgat gacccactac
    12541 gccgatctcg acgtgtcggt gctcgacgag ctgccgcccg ggcgcacgcc gatccgcacc
    12601 cgcctggtct ccgacgtgcg tcgtgacgag gtcgtcgcgc gcgtgcgcgc ggcctgtctg
    12661 gagggccgcc aggcctactg ggtgtgcccg ctgatcgagg agtccgaggc gctgcaactg
    12721 cagaccgcgc aggacacctt cgcgacgctg gtcgaggcgc tccccgagct gcgcgtgggc
    12781 ctggtgcacg gccgcctcag gaacgaggag aaatccgcca ccatggccgc cttctccgcc
    12841 ggcgcgctcg atctgctggt ggccaccacg gtgatcgagg tcggggtgga tgtgcccaac
    12901 gccagcctga tggtgatcga gcacgccgag cgcttcgggc tcgcccagct gcaccagctg
    12961 cgcggccggg tggggcgcgg cacggcggag tcggtatgca tcctgatcta cgcccagccg
    13021 ttgtcgcaga ccggccgcga gcgcctcaag gtgatctacg agaacaccga cggcttcgcg
    13081 atcgcgcgcg aggacctgcg catccgcggt ccgggcgaat tcgtcggcgc ccgccagagc
    13141 ggcgtgcccc tgctgcgcta cgccgatctc gaactcgacg ccgggctggt cgagcccgcg
    13201 cgcgagctcg ccgaacacct gctcgcccac gaccccgccg ccgccaacgc gctgatgcgg
    13261 cgctggctgg gcgggcgcga agccttgctg cgcgcatgat gggctggcga gtgctgtcgg
    13321 tgccctgtgg cacttttcgc cgcgcatcgg gtggagaacg gcagatcgcg acttgcgtcg
    13381 ctcccacgga aaacccggag cgcccggtgt gggacgcagg agcaccgcaa gtcgcgattg
    13441 ctgccgcccg gacgcaccgc atctcccgtg ggagcgacgc aagtcgcgat tgctgccgcc
    13501 cggacacccc tcgcgtgccc ttgcgtcgca agcggcctgc tcctgcacga agaaaccgaa
    13561 acccgctcct gacccgatgc gccccctgac cgccttcccc ctgcacaccg gcccgcgcca
    13621 cgagacctgg ctcgcccatc cgccgcgcgt cttgctcccg cccgcgctgc tgccctggct
    13681 gaaggatgcc ggctcgctca ccgcgcgcat ccgcgcgcgc tgcacccgct tcgaggtgcg
    13741 cgtgctgtgc cagcgcctcg cccacgtgcg gcgcgacgag gcgtggctgc tcggcctgcg
    13801 cgtcggcgag ctggcctggc tgcgcgaggt gctgctggtg gcagacggcc gtccggtggt
    13861 gttcgcgcgc tcgctgctgc cccgccgcaa cctgcgcggg gcgtggaacc tcttccacgg
    13921 catcggcgcc cgcccgctcg gcgcggcgct gttctccgac ccggcgatcg cgcgcctgcc
    13981 gctcgcctgc aggcgtctgg acgggcgcga tgcgcggtat catcgcgcgg ccgcggcgct
    14041 cgcccgccag tcccgcgcgc cggcactgcc gcgcgcgctg tgggcgcggc gctcggtctt
    14101 cctgctgcgg ggccgcgcgc tgatggtgag cgaggccttc ctccccgcaa tcctggaact
    14161 gcgcaaatga cccccgccat gagcctgagc gacaagcttc cccactatgc ccgcctgatg
    14221 cggatcgaca agccgatcgg caccctgctg ctgctgtggc ccacgctgtg ggcgctgtgg
    14281 attgccgcgg caggctggcc gccgctgcac atcctggtga tcttcatcgt cggcaccttc
    14341 ctgatgcgct ccgccggctg cgtcatcaac gactacgccg accgcgactt cgacggccac
    14401 gtcgagcgca cccgcactcg cccgctcgcc accggcgtgg tcagccccgg cgaggcgctc
    14461 gcgctcgccg ccgcgctgtc ggcggccgcc ttcgtcctcg tgctgccgct caacaccctc
    14521 accatcctgc tctcggtgcc cgcgctgctg ctcgccggca gctatccctt caccaagcgc
    14581 ttcttcgcga tcccgcaggc ctacctcggc atcgccttcg gcttcggcat cccgatggcc
    14641 ttcgccgccg tcaccggcac ggtgccggcg accggctggc tgatgctgct ggccaacatc
    14701 ttctgggctg tcgcctacga caccgaatac gcgatggtcg atcgcccgga cgacctcaag
    14761 atcggcatca agacttcggc gatcaccttc ggccgccacg acgtgctggc ggtcatgctg
    14821 tgctacgccg ccaccttcgt gctgctcacc gtcgtcggcg cgctcgccgg gctgggcttc
    14881 gcctggtacg caggcctggt cgtcgcctgc gccatcgccg gctaccacta caccctgatc
    14941 cgcgcgcgcg accgcgccga ctgcttcaag gccttccgcc acaacaactg ggtcggcggc
    15001 gcgatcttcg ccggcctctt catcgacctc ctgctgcgcg cttgagccct gtcggcgcgc
    15061 ggcgcgctct gcctcacacc ggaccgatcc ccatgcactt catgaccgcc ctccgcgccg
    15121 cctggcagca gcgcgacagc ctgctctgtg tcggcctcga ccccgacccc gccaggttcc
    15181 ccgcccacct gcaggggcgc tccgacgcca tcctccggtt ctgcacgcag atcgtcgacg
    15241 ccaccgccga cctggtgtgc tgcttcaagc cgcagatcgc ctacttcgcc gcgcaccgcg
    15301 ccgaggatca gctcgaggcg ctggtcgagc acatccacgc ccgccatccc ggcaccccgg
    15361 tcgtcctcga cgccaagcgc ggcgacatcg gcagcaccgc cgagcagtac gccatcgagg
    15421 ccttcgagcg cttcaaggcc gacgccatca cggtcaatcc ctacatgggc cgcgactcgg
    15481 tcgatcccta cctggcctac cccgacaagg gcgtgatcct gctctgccgc acctccaacc
    15541 cgggcggttc ggacctgcag tttctggagg tcgatacgtc gaagggccgc atgaagctgt
    15601 atgaacacgt cgcgcgcacc gtcgccgagg actggaacgc cagcggcaac tgcgcgctcg
    15661 tcgtcggcgc caccttcccg gccgagatcg cacgcgtgcg tgcgctcgtc ggcgagctgc
    15721 cgctgctggt gccgggcatc ggcgcccagg gcggcgacat cgccgcgacc ctggcggcgg
    15781 ggcgcacgga gaacggcacc gggctgatga tcaattcctc gcgcgcggtg ctgtatgcgg
    15841 ggaaggggga agacttcgcg gaagcggcgc ggagggtggc ggaggagacg cgggacgcga
    15901 tcaatgcgta ccgctgatcg agtccgatcg ctcgcggcgg caacttaccg gcggcgggtg
    15961 cgtcaacgcc ggccgctgct tcgcacccat cacgagacgc acgaatgaaa tacgacgacc
    16021 tccgcgactt cctcgcccag ctcgaagccc gtggcgagct ccggcgcatc aagacccccg
    16081 tcgacaccca cctc
//
LOCUS       DAOQBS010000066        16079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2710_length_16079_cov_15.323702, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000066 DAOQBS010000000
VERSION     DAOQBS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 16079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16079
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2710_length_16079_cov_15.323702"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2929)
                     /gene="metH"
                     /locus_tag="PKN25_08955"
     CDS             complement(<1..2929)
                     /gene="metH"
                     /locus_tag="PKN25_08955"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNV91069.1"
                     /translation="MQADRSIELRQLLAERILILDGAMGTMIQQEKLGEADYRGERFL
                     DHPKDLKGNNDLLVLTRPDVVAGIHRAYLDAGADLIETNTFNATRVSQAEYGLEALAY
                     ELNVEGARLVRRLCDEYTAKDPARPRFCAGVLGPTSRTLSISPDVNDPGYRNISFDAL
                     ADDYYASAKGLLEGGADILLIETIFDTLNAKAAVFAIEKLFEDLGRRWPVMISGTITD
                     ASGRTLSGQTAEAFWNSLGHAQPISFGLNCALGADQLRQYVEELSTACDTHVSAHPNA
                     GLPNPLSPTGYDETPEHLAGQIREWAQSGLVNIVGGCCGTTPAHIAAIAEAVRGLAPR
                     AVPVLEKKLRLSGLEPFNVGGDSLFVNVGERTNVTGSKAFARMILEGRFDDALAVARQ
                     QVENGAQVIDINMDEAMLDSVAAMERFCKLIATEPDISRVPIMLDSSKWSVIEAGLKC
                     IQGKGVVNSISMKEGEAEFLRQARLARRYGAAVIVMAFDEKGQADTFRRKTEICERAY
                     RLLVKPVAEGGAGFPAEDIIFDPNIFAIATGIEEHDNYAVDFINAVAWIKENLPYAKT
                     SGGVSNVSFSFRGNDPVREAIHTVFLYHAIKAGLSMGIVNAGQLGVYDELAPELRDKV
                     EDVVMNRKPGAGEALVEFAQTVKGQAKESAQDLAWREWAVEERLSHALVKGITEFVVA
                     DTEEVRAKLEAAGKPPLAVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAH
                     LIPYIEAEKLRTGASSKGRIVVATVKGDVHDIGKNIVGVVLGCNGYEVIDLGVMVPAA
                     KILEAAKEHGAQAIGLSGLITPSLEEMAHVAAEMKRQGFSVPLLIGGATTSRNHTAIK
                     IAPHYDQPVVYVPDASRAVGVVTALLSEGRSEAFKAEVAADYEKIRALHANKKGMQLV
                     SLEAARANAFRVASEYQPPRPNMLGVQAIDVELGDLVDYIDWGPFFQTWDLAGRFPAI
                     LDDE"
     regulatory      complement(3005..3079)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(3126..3683)
                     /locus_tag="PKN25_08960"
     CDS             complement(3126..3683)
                     /locus_tag="PKN25_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91070.1"
                     /translation="MLAVLLAGCASFEPPRPYTTEAEALSARGEPTRRWNNDDGTVTL
                     EYSTQPNGRSCLMVQVDRGGIVLRQWDALAPENLARVERGMDKEQVSRLLGTHRSEQR
                     FRNSGEEVWDWNIRNDGPGIAVLFNVHFIDGKVVRTSQTYVFPRDGAVLGGWSGYYGH
                     PWGWGIGWGYPGPFFPRPFGHPFFW"
     gene            complement(3763..4254)
                     /gene="bamE"
                     /locus_tag="PKN25_08965"
     CDS             complement(3763..4254)
                     /gene="bamE"
                     /locus_tag="PKN25_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNV91071.1"
                     /translation="MKRILAWLCGLLSALGITACDYINVKELKPGVSTSAEVRERFGP
                     PHMEWRNEDGSLTWEFSRQPEGIECFMITIGPDQILRRIDQVLNEETFAKVQHGMNGD
                     QVRRLLGKPASSQFFQLKQETVWEWRISRGPDGTNDPVFFTVSFNNEGRVTGTGRHTQ
                     YKR"
     gene            4267..4545
                     /locus_tag="PKN25_08970"
     CDS             4267..4545
                     /locus_tag="PKN25_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91072.1"
                     /translation="MIDGMCQRPPGRPPAMHGPGRERSDAISIDDAEPFCNRGMVRLC
                     NRTRGSQALAAAAANACISEQAARRAGGRDHFMSAIDSYLLPLTLKKA"
     assembly_gap    4575..4674
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4675..5848)
                     /locus_tag="PKN25_08975"
     CDS             complement(<4675..5848)
                     /locus_tag="PKN25_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV91073.1"
                     /translation="MKKRTVAAVVGAMLGAAVVAAPAHAQDVKLGLMAAISGPIAALA
                     PPMAAASKLAVAHVNEQGGILKGGKLEVVLGDSACNPQNATDVATKAVNIDRVIAVVG
                     PACSGAVLASANSVTIPAGVLMITPSGTSPEITKLKDKDLVYRTLPSDDYQGRALART
                     LKARGISKVAVAYLNNDYGKGLAESFKSEFEANGGTIAGYSGHEEGKASYRSELATLA
                     RGGADTLVIFDYGDGTGLSILRQSLENNFFKTFVGADGMKSEGLVKAIGAANLGGFFV
                     SAPVGEASASLDNFNKAFKAAGENIDAVFATTSYDAAFLAALAIEKAGGDKTKLAESL
                     RAVASAPGEPILAGEWAKAKKLIAEGKDIDYKGAGGDHEFDAAGDVPGNYAFFKVSG"
     gene            complement(5942..6886)
                     /locus_tag="PKN25_08980"
     CDS             complement(5942..6886)
                     /locus_tag="PKN25_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV91074.1"
                     /translation="MDLLGLANYAVFMAILIGIYALLALGLNIQWGFTGLFNAGIAGF
                     FAVGAYTSAILTSLPATGRLGGYELPLVVGWLAAMAAAALIAWPIGKICLRFRSDYLA
                     IATIGIAEIIRLVIRTEGWLTGGVRGVTGVPRPFGDLDYMPSQIAYLAIVATLVLIAY
                     LLVERQVKAPWGRMMRAIRDNELAAAAMGKQVEARRLQAFVFGSALMGLAGALFVHFN
                     RSITPEAIDPMIATFLIWIMLILGGSGNNRGAILGAAVIWIIWSVSELLTDRLPTEVA
                     VQAKYARVFIIGLTLQLVLRFRPEGILPERQAGSPPHR"
     gene            complement(6886..7755)
                     /locus_tag="PKN25_08985"
     CDS             complement(6886..7755)
                     /locus_tag="PKN25_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV91075.1"
                     /translation="MSFMDFLNFHIVPGVVLGSIYAVGAIGITLVFGILRFSHFAHGD
                     MATLGAFVALALVTAGLTPWAALPIAMAAVAVVAIVVDKLFYDYLKARPKIMTVMASL
                     GVALMLRAVVQVVFGVDPRTYASGIVRPDEYFGILLRDRELYTLAAVVLIVVGLTLFL
                     RHSRWGKAMRAMSDNPDLARLSGVDNRKVTMLTWAIVGALCAASGFMLGINNELHSMM
                     GWQVLLPMFAAAILGGVGRVEGAVLGGLIVGIAEELSIFILPAQYKAATAFALLLLIL
                     LVRPRGLLNGKVL"
     gene            complement(7771..8475)
                     /locus_tag="PKN25_08990"
     CDS             complement(7771..8475)
                     /locus_tag="PKN25_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV91076.1"
                     /translation="MALLEMKDVRGGYGDADILHGVSMTVGEREIVVIVGPNGAGKST
                     AMKSVFGLLTIRGGNIVFDGEDITGWAPDRIVQRGICYVPQVDNVFREMTVHENFEMG
                     AFLRRGDLAAAYDRIYGLFPDLKAKRRNLAGDLSGGQRQMVAMGRALMLDPKLLLLDE
                     PTAGLSPKYMEQIFQITRDVRDAGVSILLVEQHAKQALAFCDRGYVLATGANRHEGSG
                     QALLADREVAEMFLGG"
     gene            complement(8475..9314)
                     /locus_tag="PKN25_08995"
     CDS             complement(8475..9314)
                     /locus_tag="PKN25_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV91077.1"
                     /translation="MLEVDGLVKAFGGFHAVDGCTLRVAKGEILGLIGPNGAGKTTLF
                     NLVAGALQPTAGTIRFLGEDITALPTDARFHKGLVRTFQIPHEFHNLTARENLMMVPP
                     RQPGENLFANWFAAGKVRAAEEAVRQKADDTLAFLELTHVADERAGNLSGGQKKLLEL
                     GRTMMTDAKLVLLDEPAAGVNRTLLRKLEEKILILNRERGYTFILIEHDMEMIEKLCA
                     PVVCMAEGKVLIQGDFHTVRSDSRVLEAYLGETPTAVEREAAEHDPKVEELREQELHR
                     EDL"
     gene            complement(9502..10710)
                     /locus_tag="PKN25_09000"
     CDS             complement(9502..10710)
                     /locus_tag="PKN25_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNV91078.1"
                     /translation="MIRPLTHARLASLLAALSSIGPFAIDAYLPAFPAIAAALGASRL
                     EVQQTLTIYMVALASMVLWHGALADRFGRRRVLVALTVVFALASLVCALAPSIEWLWF
                     GRALQGLSGGAGIVVGRAVVRDLHEGPQAQRLMSRVMLIFALAPALAPMLGAGLLALA
                     GWRAIFFFLALFGLALAWTTWRFLPETLAEEDRQPLHPRPLLRAYLAIFRHPAFMLLA
                     LGVALNFNGFFLYVLSAPVFILEHLSLGSGGFVWLFGPAVGGMMIGSTLSERVAGRWS
                     PVRTVGFGFVLMFAAVVANAAVSGLLVPAVPVSVLPIGLFSCGMAVAMPSMSLLGLEI
                     FPERRGMASSCQSFLQIGFNAASSALVAPLLWSSPLGLASGMGMFTALGLCAWMAWWR
                     LGAHVREGAA"
     gene            complement(10777..12279)
                     /locus_tag="PKN25_09005"
     CDS             complement(10777..12279)
                     /locus_tag="PKN25_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNV91079.1"
                     /translation="MSLALVHARALDGIEAPPVTVEVHLGNGLPQFHLVGLADTEVRE
                     ARDRVRAAIQSAQFEFPQRRITVNLAPADLPKEGGRFDLAIAIGILAASGQVAERLLP
                     RLELCGELSLNGGLRPVRGVLSAALAAARAGRALVLAPANAAEAALARSATVLPADSL
                     GAVCAHLNGHTRLEPALAAAPEDEDAAGLPDLADVRGQLQARRALEVAAAGGHSLLLF
                     GPPGTGKSMLAQRLPGVLPAMDEDEAIEAAAVASIEGRLDPRSFHRRPFRSPHHSASA
                     AALVGGGAVPRPGEISLAHHGVLFLDELPEFDRRVLESLREPLETGTVTVSRARRRAE
                     FPARFQLVAAMNPCPCGHLGDPRRACRCTPEQVARYRGRLSGPFLDRIDLTVEVPAIA
                     PEVLLESPPGEPSSAVRARVAAARALQATRQGCPNARLPAGEVERHCRPDPGGETLLR
                     QAMQRLDLSARAYHRVLRVARTLADLAGASRPGAAQVAEAIQYRRSLDSR"
     gene            complement(12551..12802)
                     /locus_tag="PKN25_09010"
     CDS             complement(12551..12802)
                     /locus_tag="PKN25_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HNV91080.1"
                     /translation="MSTPRILDEIGARLSELAANSPVRDIEKNAKAMLGSAFGKLDLV
                     TREEFEVQREVLAQARRKLAELEARVAELEAKLAGGDKA"
     assembly_gap    13072..13171
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13381..14085
                     /locus_tag="PKN25_09015"
     CDS             13381..14085
                     /locus_tag="PKN25_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HNV91081.1"
                     /translation="MHTKPLATALAILATIATAGTAHAGDGPFTANVGLVSDYAYRGW
                     SQTDERPALQGGFDYAHDSGLYAGVWGSNVSWLSDANTDVSNSLELDLYGGYKGTVGA
                     IGYDVGLLQYYYPGSYPKPYNSPNTLEGYVGLSWEFLSFKYSYAFTDLFGYDKSDGSQ
                     YYDLGAAVDVGGGFTLAAHVGYSDIKGQDDYTDWKLGVTKEFGGFNFGLHYVDTDVDN
                     ADLADERVILSIAKSF"
     gene            14115..14453
                     /gene="glnK"
                     /locus_tag="PKN25_09020"
     CDS             14115..14453
                     /gene="glnK"
                     /locus_tag="PKN25_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNV91082.1"
                     /translation="MKFIAAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEAAIPDDILDQAIEAIEKSAATGKIGDGKIFVFELEQAIRI
                     RTGETGPDAL"
     gene            14564..15790
                     /gene="amt"
                     /locus_tag="PKN25_09025"
     CDS             14564..15790
                     /gene="amt"
                     /locus_tag="PKN25_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNV91083.1"
                     /translation="MHKGDVAWIMTSTLLVLMMALPGLALFYGGMVRAKNMLSVLMQV
                     MTVFSLNALLFAIYGYSLTFTEASPFIGSLDKVFLSGVTIETLADTFTETVKLPEFAF
                     IAFQATFAGITGALIVGAFAERMKFSAVLIFSVIWFTLCYLPICHMVWGPGGYLLEDG
                     ALDFAGGTVVHINAGIAGLVGAWMVGKRIGYGREALAPHSLTLTMVGASLLWVGWFGF
                     NAGSNLEATAGAALAFINTLVATAAAVLAWSLGEALIKGKPSMLGAASGAVAGLVAIT
                     PACGSVGPMGAIVIGTLSGFICLWGVNGLKHMLGADDALDVFGVHGVGGIVGAILTGV
                     FAAPSLGGTGAEDFSIASQVWIQTWSVLVTIVWSAVVAFVAYKVADLLVGLRVPEDEE
                     RQGLDTTAHGETAYRY"
     gene            15874..>16079
                     /locus_tag="PKN25_09030"
     CDS             15874..>16079
                     /locus_tag="PKN25_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91084.1"
                     /translation="MEAIGRNALPAFAQRKPASGTDTASLPFDPPHDDIGRIPGVQST
                     PRQRPMRHKIAPLRLLSLAALVTG"
BASE COUNT         2574 a         5457 c         5457 g         2391 t
ORIGIN      
        1 cttcgtcgtc gaggatggcg gggaagcggc cggcgaggtc ccaggtctgg aagaaggggc
       61 cccagtcgat gtaatcgacc aagtcgccga gctcgacgtc gatcgcctgc acgcccagca
      121 tgttcgggcg cggcggctgg tattcggagg cgacgcggaa ggcgttggcg cgcgcagcct
      181 cgaggctcac caactgcatg cccttcttgt tggcgtgcag cgcgcggatc ttctcgtagt
      241 cggcggcgac ctcggccttg aaggcctcgc tccggccttc ggacagcagc gcggtgacca
      301 cgcccaccgc acgcgaggcg tcgggcacgt acaccaccgg ctgatcgtag tgcggcgcga
      361 tcttgatcgc ggtgtggttg cggctggtgg tggcgccgcc gatcagcagc ggcacggaaa
      421 agccctgacg cttcatctcg gcggcgacgt gggccatctc ctcgagcgag ggcgtgatca
      481 ggccggacag gccgatcgcc tgcgcgccgt gctccttcgc cgcctcgagg atcttcgctg
      541 ccggcaccat cacgccgagg tcgatcacct cgtagccatt gcagccgagc accacgccga
      601 cgatgttctt gccgatgtcg tgcacgtcgc ccttgaccgt ggcgacgacg atacggccct
      661 tgctgctggc gccggtgcgc agcttctcgg cctcgatgta ggggatcagg tgcgccaccg
      721 cctgcttcat gacgcgcgcc gacttcacca cctggggcag gaacatcttg cccgcgccga
      781 agaggtcgcc gaccacgttc atgccggcca tcagcgggcc ctcgatcacc gccagcggcg
      841 gcttgcccgc ggcctccagc ttcgccctca cctcctcggt atcggcgacg acgaactcgg
      901 tgatgccctt gaccagcgcg tggctcaagc gctcctcgac cgcccactcg cgccaggcga
      961 ggtcctgcgc ggactccttg gcctggccct tcaccgtctg cgcgaactcg accagcgcct
     1021 cgcccgcccc gggcttgcgg ttcatcacca cgtcctcgac cttgtcgcgc agctcgggcg
     1081 cgagctcgtc atagacgccg agctggccgg cattgacgat gcccatcgac aggccggcct
     1141 tgatcgcgtg gtagaggaac acggtgtgga tcgcctcgcg caccgggtcg ttgccgcgga
     1201 agctgaagga cacgttcgac accccgcccg aggtcttggc gtacggcagg ttctccttga
     1261 tccaggccac cgcgttgatg aagtcgaccg cgtagttgtc gtgctcctcg atgccggtgg
     1321 cgatggcgaa gatgttgggg tcgaagatga tgtcctcggc cgggaagccg gcgccgccct
     1381 cggccaccgg cttcaccagc agcctgtagg cgcgctcgca gatctcggtc ttgcggcgga
     1441 aggtgtcggc ctgacccttt tcgtcgaagg ccatcacgat cactgccgca ccatagcggc
     1501 gtgcgagccg cgcctggcgc aggaactctg cctcgccctc cttcatcgag atcgagttga
     1561 ccacgccctt gccctggatg cacttgaggc cggcctcgat caccgaccac ttcgacgagt
     1621 cgagcatgat cggcacacgc gagatgtcgg gctcggtggc gatcaacttg cagaagcgct
     1681 ccatcgcggc gaccgaatcc agcatcgcct cgtccatgtt gatgtcgatg acctgggcac
     1741 cgttttcgac ctgctggcgc gccaccgcga gcgcgtcgtc gaagcggccc tcgaggatca
     1801 tgcgcgcgaa ggccttggaa ccggtgacgt tggtgcgctc gccgacgttg acgaaaagac
     1861 tgtcgccgcc gacgttgaag ggctccagcc cggacaggcg cagctttttc tccagcaccg
     1921 gcacggcgcg cggcgccagg ccccggaccg cttcggcgat cgccgcgatg tgcgccggcg
     1981 tggtgccgca gcagccgccg acgatgttca ccaggccgct ctgcgcccac tcgcgaatct
     2041 ggccggcgag gtgctcgggc gtctcgtcgt agccggtggg cgacagcggg ttgggcaggc
     2101 cggcgttggg gtgggcggac acgtgggtgt cgcaggcggt ggagagttcc tcgacgtact
     2161 ggcgcagctg gtcggcgccg agcgcgcagt tgaggccgaa gctgatcggc tgcgcgtggc
     2221 cgagcgagtt ccagaaggcc tccgcggtct ggcccgacag cgtgcggccc gaggcgtcgg
     2281 tgatggtgcc ggagatcatc accggccagc gccggccgag gtcctcgaac agcttctcga
     2341 tcgcgaacac cgccgccttg gcattcagcg tatcgaagat ggtctcgatc agcaggatgt
     2401 cggcaccgcc ttcgagcagg cccttcgccg aggcgtagta gtcgtcggca agcgcatcga
     2461 agctgatgtt gcggtagccg ggatcgttca catcgggcga gatcgacagc gtgcgcgagg
     2521 tcggcccgag cacgccggcg cagaagcgcg gcctggcggg gtcctttgcg gtgtattcgt
     2581 cgcacagccg gcgcaccagg cgggcgccct cgacgttgag ctcgtaggcc agcgcctcca
     2641 ggccgtactc cgcctgcgac acgcgggtgg cgttgaaggt gttggtctcg atgaggtcgg
     2701 cgccagcgtc cagataggcg cggtggatgc cggcgaccac gtccggacgc gtgagcacca
     2761 gcagatcgtt gttgcccttg aggtccttgg ggtggtcgag gaagcgctcg ccgcggtagt
     2821 ccgcctcgcc cagcttctcc tgctggatca tcgtgcccat ggcgccgtcc aggatcagga
     2881 tgcgctcggc gagaagctgg cggagttcga tgctgcggtc ggcttgcatg ggatgtcctc
     2941 ggtcgttcga cggtcgccgg gcaacgccac gcacaaatgc gaacggcgcc acaaaccggc
     3001 tgaaggtttg tgagcgccgt tgatcggttg ccgagcctgg ccccggatcg atcagggggt
     3061 cgcagcgctc ctcggcaggc ccaggattct atcggcagca tgcgcccgct gccaagggcg
     3121 gcggcctacc agaagaaggg gtgcccgaac ggccgcggga agaaggggcc gggataaccc
     3181 cagccgatgc cccagcccca gggatggccg tagtagccgc tccagccccc gaggacggcg
     3241 ccgtcacgcg ggaacacgta ggtctggctg gtccgcacca ccttgccgtc gatgaagtgc
     3301 acgttgaaca ggacggcgat cccaggaccg tcgttgcgga tgttccagtc ccacacctcc
     3361 tcgcccgagt tgcggaagcg ctgctcggag cggtgggtgc cgagcaggcg cgagacctgc
     3421 tccttgtcca tgccgcgctc gacgcgggcg agattctcgg gggcgagcgc atcccactgg
     3481 cgcagcacga tcccgccccg gtcgacctgc accatcaggc agctgcgacc gttgggctgg
     3541 gtggaatact ccagcgtgac cgtgccgtcg tcgttgttcc agcgccgcgt gggctcaccg
     3601 cgcgcggaca gggcctcggc ctcggtggtg tagggacgcg gcggctcgaa gctcgcgcaa
     3661 cctgcgagca ggacggccag caatacaggc cacagccagc acagcggtga agcctgggcg
     3721 cggacggacg gggtgctggg gaaatacatc gaggcctccc tgctaacgct tgtactgggt
     3781 atgtcttccg gttccggtca ctcgcccttc gttgttgaac gacaccgtga agaagaccgg
     3841 atcgttcgtg ccgtcgggac cgcgcgagat ccgccactcc cacaccgtct cctgcttgag
     3901 ctggaagaac tggctggacg ccggcttgcc aagcaggcga cgcacctggt cgccattcat
     3961 gccgtgttgg accttggcga aggtctcctc gttcagcacc tggtcgatgc ggcgcaggat
     4021 ctggtcgggg ccgatggtga tcatgaagca ctcgatcccc tccggctgac ggctgaactc
     4081 ccaggtgagc gagccgtcct cgttgcgcca ttccatgtgt ggcgggccga agcgctcgcg
     4141 cacctcggcg gaggtggaca ccccgggctt gagctccttc acgttgatat agtcgcaggc
     4201 cgtgatgccg agcgcggaca gcaggccaca aagccaggcg aggatgcgtt tcatccggat
     4261 tctccgttga tcgacggcat gtgccagcgg ccgccgggac gccctccagc catgcacggg
     4321 cccggccggg agcgcagcga cgccatcagc atcgatgatg ccgagccgtt ttgcaatcgg
     4381 ggaatggtga ggctttgcaa tcgcacgcgg gggtcgcaag cgctcgcggc ggcggccgcg
     4441 aacgcttgca tttcggaaca agccgcccgc cgagcgggcg ggcgcgatca cttcatgtcg
     4501 gcgatcgact cgtacctgtt gccgctgacc ttgaagaagg cgtagttgcc cggcacgtcg
     4561 ccggcggcat cgaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgccgc
     4681 tgaccttgaa gaaggcgtag ttgcccggca cgtcgccggc ggcatcgaac tcgtggtcgc
     4741 cgccggcgcc cttgtagtcg atgtccttgc cttcggcgat cagcttcttg gccttggccc
     4801 actcgcccgc caggatcggc tcgcccggcg cactggccac cgcgcgcagc gactcggcca
     4861 gcttggtctt gtcgccccct gccttctcga tcgccagcgc ggcgaggaag gcggcgtcgt
     4921 aggaggtggt ggcgaacacg gcgtcgatgt tctcgcccgc ggccttgaag gccttgttga
     4981 agttgtcgag cgaggccgag gcctcgccca ccggggcgga cacgaagaag ccaccgaggt
     5041 tggccgcgcc gatcgccttg accagaccct cggacttcat gccgtcggcg ccgacgaagg
     5101 tcttgaagaa gttgttctcg agcgactggc gcaggatgct caggcccgtg ccatcgccgt
     5161 aatcgaagat gaccagggtg tcggcgccac cgcgggcgag cgtggccagc tcggagcggt
     5221 aggaggcctt gccctcctcg tggcccgaat agccggcgat ggtgccgccg ttggcctcga
     5281 actcggactt gaaggattcg gccaggccct tgccgtaatc gttgttcagg taggccaccg
     5341 ccaccttgga gatgccgcgc gccttgaggg tgcgcgccag ggcgcggccc tggtagtcgt
     5401 cggacggcag cgtgcggtag acgaggtcct tgtccttgag cttggtgatc tcgggcgagg
     5461 tgccggaagg ggtgatcatc agaacgccgg cgggaatggt caccgagttc gccgaggcga
     5521 gcaccgcgcc cgagcaggcc ggaccgacga ccgcgatcac gcggtcgatg ttcaccgcct
     5581 tggtggcgac gtcggtggcg ttctgcgggt tgcaggcgct gtcgccgagc acgacctcga
     5641 gcttgccgcc cttgaggatg ccgccctgtt cgttgacgtg ggcgaccgcc agcttggagg
     5701 ccgccgccat cggcggcgcg agcgccgcga tcgggcccga gatggccgcc atcaggccga
     5761 gcttgacgtc ctgggcgtgg gccggtgcgg cgacgaccgc ggcgccgagc atggcgccga
     5821 ccactgctgc gactgttctt ttcttcacct ttgtcacctc ctgaatgtgc cgaagcacct
     5881 gatggacgat gcgggcccgc cgcgggcccg ccacgaagtt ctggtcacgg tcgcgcgcag
     5941 ctcaacgatg cggcggcgaa cccgcctggc gctcgggcag gatgccctcg gggcggaagc
     6001 gcagcaccag ctgcaaggtc aggccgatga tgaacacgcg cgcatacttg gcctgcaccg
     6061 cgacctcggt gggcaggcgg tcggtgagca gctcggacac cgaccagatg atccagatga
     6121 ccgccgcgcc gaggatggca ccgcggttgt tcccggagcc gcccaggatc agcatgatcc
     6181 agatgaggaa agtggcgatc atggggtcga tcgcctcggg ggtgatcgag cggttgaagt
     6241 gcacgaacag cgcgcccgcc agccccatca gcgccgagcc gaacacgaag gcctgcagcc
     6301 ggcgcgcctc gacctgcttg cccatcgccg cggcggcgag ctcgttgtcg cggatcgccc
     6361 gcatcatgcg cccccacggc gccttcacct ggcgctcgac gagcaggtag gcgatcagca
     6421 ccagggtggc gacgatcgcc aggtaggcga tctgcgaggg catgtagtcg aggtcgccga
     6481 agggacgggg cacgccggtg acgccgcgca cgccgccggt gagccagcct tcggtgcgga
     6541 tcacaaggcg gatgatctcg gcgatgccga tggtggcgat ggcgaggtag tcggagcgga
     6601 agcgcaggca gatcttgccg atcggccacg cgatcagcgc cgccgccgcc atcgccgcca
     6661 gccagccgac cacgagcggc agctcgtaac cgcccagccg gccggtggcg ggcaggctgg
     6721 tgaggatcgc ggaggtgtag gcgccgaccg cgaagaagcc cgcgatgccg gcgttgaaaa
     6781 ggccggtgaa gccccactgg atgttcagcc cgagcgcgag cagcgcgtag atgccgatca
     6841 ggatggccat gaagaccgcg tagttggcga gtccgagcag atccatcaga gcaccttgcc
     6901 gttgagcagg ccgcgcgggc ggacgagcag gatcagcagc agcagcgcga aggcggtggc
     6961 cgccttgtac tgcgccggca ggatgaagat cgacagctcc tcggcgatgc ccacgatcag
     7021 gccgccgagc accgcgccct cgacccggcc cacgccgccg aggatggcgg cggcgaacat
     7081 cggcagcagc acctgccagc ccatcatcga gtgcagctcg ttgttgatgc cgagcatgaa
     7141 gcccgaggcc gcgcacagcg cgccgacgat cgcccaggtg agcatggtga ccttgcggtt
     7201 atccacgccc gaaagccgcg cgaggtcggg gttgtccgac atcgcccgca tcgccttgcc
     7261 ccagcgcgaa tggcgcagga acagggtgag gccgaccacg atcagcacca ccgccgccag
     7321 cgtgtacagc tcgcggtcgc gcagcaggat gccgaagtat tcgtccgggc gcacgatgcc
     7381 gctggcgtag gtgcgcggat cgacgccgaa caccacctgc accaccgcgc gcagcatcag
     7441 cgcgacgccg agcgaggcca tcacggtcat gatcttgggc cgtgccttga ggtagtcgta
     7501 gaacagcttg tcgacgacga tcgccaccac cgcgaccgcc gccatcgcga tcggcagcgc
     7561 cgcccaaggg gtgagcccgg cggtgaccag ggcgagcgcg acgaaggcgc ccagcgtggc
     7621 catgtcgccg tgggcgaagt gggagaagcg caggatgccg aacaccagcg tgatcccgat
     7681 cgcgcccacc gcgtagatcg agccgagcac gacgccgggc acgatgtgga agttgaggaa
     7741 gtccatgaag ctcatgagcg ccggactccc ttagccgccg aggaacattt cggccacctc
     7801 gcgatccgcg agcagggcct gtccgctgcc ctcgtggcgg ttggcgccgg tggcgagcac
     7861 gtagccgcgg tcgcagaagg cgagcgcctg cttggcgtgc tgctcgacga gcaggatgga
     7921 gacaccggcg tcgcgcacgt cgcgggtgat ctggaagatc tgttccatgt acttggggga
     7981 gaggccggcg gtgggttcgt cgagcagcag cagcttggga tcgagcatca gcgcgcgccc
     8041 catcgccacc atctggcgct ggccgccgga aaggtcgccg gcaaggttgc ggcgcttggc
     8101 cttgaggtcg gggaagagcc cgtagatgcg atcgtaggcg gcggcgaggt cgccgcggcg
     8161 caggaaggcg cccatctcga agttctcgtg caccgtcatc tcgcggaaca cgttgtccac
     8221 ctgcggcacg tagcagatgc cgcgctgcac gatgcggtcg ggcgcccagc cggtgatgtc
     8281 ctcgccgtcg aagacgatat tgccgccgcg gatcgtgagc aggccgaaca cggatttcat
     8341 cgcggtggac ttgccggcgc cgttggggcc gacgatgacg acgatctcgc gctcgcccac
     8401 ggtcatcgac accccgtgca ggatgtcggc gtcgccgtag ccgccgcgca cgtccttcat
     8461 ttcgaggagc gccatcacag gtcctcccgg tgcagttcct gctcgcgcag ttcctcgacc
     8521 ttggggtcgt gctcggcagc ctcgcgctcg accgcggtcg gcgtctcgcc gaggtaggcc
     8581 tcgagcacgc gggaatcgga gcgcactgta tggaaatcgc cctggatcag caccttgccc
     8641 tcggccatgc acaccaccgg agcgcacagc ttctcgatca tctccatgtc gtgctcgatg
     8701 aggatgaagg tgtagccgcg ctcgcggttg aggatgagga tcttctcctc gagcttgcgc
     8761 agcagggtgc ggttgacacc ggcggcgggc tcgtcgagca acaccagttt ggcgtcggtc
     8821 atcatcgtgc ggccgagctc gagcagtttc ttctgcccgc cggacaggtt gcccgcgcgc
     8881 tcgtcggcga cgtgagtcag ttccaggaag gcgagcgtgt cgtcggcctt ctggcgcacc
     8941 gcctcttcgg ccgcgcgcac cttgccggcg gcgaaccagt tggcgaagag gttctcgccg
     9001 ggttgccgcg gcggcaccat catcagattc tcgcgcgcgg tgaggttgtg gaactcgtgg
     9061 gggatctgga aggtgcgtac caggcctttg tggaagcgcg cgtcggtagg cagcgcggtg
     9121 atgtcctcgc cgaggaagcg gatggtgccc gcggtcggct gcagggcgcc ggcgaccagg
     9181 ttgaacagcg tggtcttgcc cgcgccgttg gggccgatga ggccgaggat ctcgcccttg
     9241 gccacccgca gggtgcagcc atccacggca tggaagccgc cgaaggcttt gaccaggcca
     9301 tccacttcga gcatagcgtt gcgattcctt gcggcgagaa gagcgcgaca gaggcggcgt
     9361 gagccggccc accggcacat gaacgtgacg ccccgagcag ggcggtcgat aacacacgga
     9421 tactagaacg aggcgtgacc ttagccgaat gcatccgcga caaaaatagg ggccaaccac
     9481 tacgggcgtg gcgcgacctt gctacgccgc accctcgcgc acgtgcgcgc ccagccgcca
     9541 ccatgccatc cacgcacaca ggccgagcgc ggtgaacatg cccatccccg acgccagccc
     9601 gagcggactc gaccacaaca gcggcgccac cagcgccgag cttgccgcgt tgaagccgat
     9661 ctgcaggaag ctctggcagc tcgacgccat tccgcggcgc tcgggaaaga tctccagccc
     9721 gagcaggctc atgctcggca tcgccaccgc catgccgcag ctgaacaagc cgatcggcag
     9781 caccgagacc ggcaccgcgg ggacgaggag gcccgacacc gcggcgttgg ccaccaccgc
     9841 cgcgaacatc aagacgaagc cgaagcccac cgtgcgcacc ggcgaccagc gcccggccac
     9901 gcgctcggag agcgtcgagc cgatcatcat cccgcccacc gccggaccga acagccacac
     9961 gaaaccgcca ctgcccaggc tcagatgctc gaggatgaac accggcgccg agagcacgta
    10021 gaggaagaaa ccgttgaagt tcagcgccac cccgagcgcc agcaacatga aggccgggtg
    10081 gcggaagatc gccaggtagg cgcgcagcag cggccgcgga tgcagcggct ggcggtcctc
    10141 ctcggcgagc gtctcgggca ggaagcgcca cgtcgtccac gcgagcgcga gcccgaaaag
    10201 ggccaggaag aagaagatcg cccgccagcc tgcgagcgcg agcaggcccg cgcccagcat
    10261 cggcgccagc gccggagcga gcgcgaagat cagcatcacc cgcgacatca ggcgctgcgc
    10321 ctgcggcccc tcgtgcaggt cgcgcaccac cgcacgcccg accacgatgc ccgcgccgcc
    10381 gctcagcccc tgcagcgcgc gcccgaacca cagccactcg atgctcggcg ccagcgcgca
    10441 caccagcgag gcgagcgcga acaccaccgt caacgcgacc agcacgcggc gacgcccgaa
    10501 acggtcggcg agcgcaccgt gccatagcac catggacgcg agcgccacca tgtagatcgt
    10561 cagcgtctgc tgcacctcca gccggctcgc gcccaacgcc gccgcgatcg ccggaaaggc
    10621 gggcagatag gcatcgatcg cgaacgggcc gatcgaggac agggctgcca gcagcgaagc
    10681 gaggcgggcg tgggtgagcg gtcggatcat gtacgccttc gggcggactc ctggtgtcgt
    10741 cgtccgcagc cgacggcgca cggggcgcgg ggtcgttcag cgggagtcga ggctgcgccg
    10801 gtactggatc gcctcggcca cctgggctgc ccccggacgg gacgcgccgg cgagatcggc
    10861 gagcgtgcgc gcgacgcgca ggacacgatg atacgcccgc gccgacaggt cgagccgctg
    10921 catcgcctgg cgcagcaggg tttcgccgcc cggatcgggg cgacagtggc gctccacctc
    10981 gccggccggc aggcgggcgt tcggacagcc ctgacgcgtt gcctgcagcg cacgcgccgc
    11041 cgccacccgc gcgcgcaccg cgctggaggg ctcgcccggc gggctttcga gcaggacctc
    11101 cggcgcgatt gcaggcacct cgacggtgag gtcgatgcgg tcgagaaagg ggccggacag
    11161 ccgcccgcgg tagcgcgcca cctgctcggg cgtgcaccgg caggcgcggc gcggatcacc
    11221 gagatggccg cagggacagg gattcattgc cgcgacgagc tggaagcggg cggggaattc
    11281 ggcgcgccgg cgcgcgcgcg acaccgtgac ggtgccggtc tcgagcggct cgcgcaggga
    11341 ctcgagcaca cggcggtcga actcgggcag ctcgtcgagg aacagcacgc cgtggtgcgc
    11401 gagcgagatc tcgcccgggc gcggcaccgc gccgccgccc accagcgccg ccgccgaggc
    11461 cgagtgatgc ggcgaccgaa aaggtctgcg gtggaagctg cgcggatcga ggcgcccctc
    11521 gatcgaggcc accgcggcgg cctcgatcgc ctcgtcctcg tccatcgccg gcagcacgcc
    11581 gggcaggcgc tgcgccagca tcgacttgcc ggtacccggc ggtccgaaca gcagcaggga
    11641 atgcccgccc gccgcggcga cctcgagcgc acgccgcgcc tgcagttgac cgcgcacatc
    11701 ggcgagatcg ggcagcccgg cggcgtcctc gtcctcgggg gccgccgcga gcgccggctc
    11761 gaggcgggtg tgtccgttga gatgcgcgca caccgcgccc aggctgtcgg ccgggagcac
    11821 ggtggcgctg cgcgcgagcg cggcctcggc cgcattggcg ggcgccagca ccagcgcgcg
    11881 tccggcgcgc gcggcggcga gcgctgccga gagcaccccg cgcaccgggc gcaatccgcc
    11941 gttcagcgaa agctcgccgc acagctccag gcgcgggagc agccgctcgg ccacctggcc
    12001 ggaggcggcg aggatgccga tcgcgatcgc gagatcgaag cgcccgccct ccttgggcag
    12061 gtctgcaggc gccaggttga cggtgatgcg gcgctgggga aactcgaact gcgccgactg
    12121 gatcgccgca cgcacgcggt cgcgcgcctc gcgcacctcg gtgtcggcga ggccgacgag
    12181 atggaactgg ggcaggccgt tgcccaggtg gacctcgacc gtgaccgggg gcgcctcgat
    12241 cccgtcgagc gcgcgcgcat gaacgagggc gagcgacatc tgcgactccg gacggaaagg
    12301 aatcgtcagt ctagcggagc cgcggtcgat gacaaagaaa tatgcggaac gcgggaccgg
    12361 gggcattcgg tcacgagacg catacggtgt ttcatgcaga cgcgagcgca gcatgtagcg
    12421 tgtcgcgacg cccatcgccg cccaccgtcc gttcgcggct ggcgccgctc ctacgaggtt
    12481 tccgggaacc ttgccggttt ccgcaggagc gccgccagac gcgaatgcag cgtgtggaaa
    12541 cgcagctgga tcaggccttg tcgccgccgg ccagcttcgc ttccagttcg gcgacgcgcg
    12601 cctcgagctc ggcgagcttg cgccgcgcct gcgcgagcac ctcgcgctgc acctcgaatt
    12661 cctcgcgggt gaccaggtcg agcttgccga aggccgagcc gagcatggcc ttggcgttct
    12721 tctcgatgtc gcggacgggg ctgttggcgg cgagttcgga gaggcgggca ccgatttcgt
    12781 cgaggatgcg gggcgtgctc atgatggcgt ggattccggg ttgcagaggc gtcggagtct
    12841 agcacgcacg cccctgccga ctggagcgcc ggcggcccgc cgcacacgat cggcgcaggc
    12901 gcggtgcgcg cgacattgcc gtgatcacga cttgcgtcgc tccggcggga gcccgggcga
    12961 cgcaggggtg ggcgaaggtt ttcgtaggag cgacgcaagt cgcgatctgg cgccaagaac
    13021 cgtcaaccat cgctcgcaca acacgaatga cgcaccacat tggtgcgcaa cnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaaccgtca accatcgctc gcacaacacg
    13201 aatgacgcac cacattggtg cgcaacgccc cgaaatcgcg cgcccatccc tgccgcagcg
    13261 cagcaagatc cgcccgccga caagcggaaa cctaggaatc accaggcttt ccgcaccctg
    13321 gcacgccacg tgcttaggga gtctcgtcgc gaactttcca aacctcgtca ggagatgacc
    13381 atgcacacga aaccgctcgc caccgcgctc gccatcctcg ccacgatcgc caccgccggc
    13441 accgcccatg ccggcgacgg ccccttcacc gccaacgtcg gcctggtgtc cgactacgcc
    13501 taccgcggct ggagccagac cgacgagcgc cccgcgctgc agggcggctt cgactacgcc
    13561 cacgacagcg gcctgtacgc cggcgtgtgg ggctcgaacg tgtcgtggct gtcggacgcc
    13621 aacacggacg tcagcaacag cctcgagctc gacctctacg gcggttacaa ggggacggtc
    13681 ggcgccatcg gctacgacgt cggcctgctg cagtactact accccggcag ctaccccaag
    13741 ccctacaaca gcccgaacac gctcgagggc tatgtcggcc tgtcgtggga gttcctgtcc
    13801 ttcaagtact cgtacgcctt caccgacctg ttcggctacg acaagtcgga cggcagccag
    13861 tactacgacc tcggcgccgc ggtcgatgtc ggcggcggct tcaccctcgc cgcccacgtc
    13921 ggttacagcg acatcaaggg ccaggacgac tacaccgact ggaagctggg cgtcaccaag
    13981 gagttcggcg gcttcaactt cggcctgcac tacgtcgaca ccgacgtgga caacgccgac
    14041 ctcgccgacg agcgcgtgat cctgtcgatc gccaagagct tctgagcatc gcccatccgg
    14101 aaccggagaa agacatgaaa ttcatcgctg caatcatcaa gcccttcaag ctcgacgagg
    14161 tccgcgaggc gctctcggcc atcggcgtgc agggcatcac cgtgaccgag gtcaagggct
    14221 tcggccgcca gaagggccac accgagctct atcgcggcgc cgagtacgtc gtcgacttcc
    14281 tgcccaaggt caaggtcgag gccgccatcc ccgacgacat cctcgaccag gcgatcgaag
    14341 cgatcgagaa gtccgccgcc accggcaaga tcggcgacgg caagatcttc gtcttcgaac
    14401 tcgagcaggc catccgcatc cgcaccggcg agaccggccc ggacgcgctg taagggagca
    14461 acgcaaaatg agaaaactcc tcgcatacct gccggcagca gccctgctcg ccggcgtctc
    14521 cggcggcgcc ttcgcccagg aggccgcggc cgccgtggcc gaggtgcaca agggcgacgt
    14581 ggcctggatc atgacctcca cgctgctcgt gctgatgatg gccctgcccg gcctggcgct
    14641 gttctacggc ggcatggtgc gcgccaagaa catgctctcg gtgctcatgc aggtcatgac
    14701 cgtgttctcg ctcaacgccc tgctgttcgc gatctacggc tacagcctga ccttcaccga
    14761 agccagcccc ttcatcggct cgctcgacaa ggtcttcctg tccggggtga cgatcgagac
    14821 gctcgccgac accttcaccg agaccgtgaa gctgcccgag ttcgccttca tcgccttcca
    14881 ggccaccttc gccggcatca ccggtgcgct catcgtcggc gccttcgccg agcgcatgaa
    14941 gttctccgcg gtgctgatct tctccgtgat ctggttcacg ctgtgctacc tgccgatctg
    15001 ccacatggtg tggggcccgg gcggctacct gctcgaagac ggcgcgctcg acttcgccgg
    15061 cggcaccgtg gtgcacatca acgccggcat cgccggtctg gtgggcgcct ggatggtggg
    15121 caagcgcatc ggctacggcc gtgaggcgct cgcaccgcac agcctgaccc tgaccatggt
    15181 cggcgcctcg ctgctgtggg tgggctggtt cggcttcaac gccggctcca acctcgaagc
    15241 caccgccggc gccgcgctcg ccttcatcaa caccctggtc gccaccgccg ccgcggtgct
    15301 cgcctggtcg ctcggcgagg cgctcatcaa gggcaagccc tcgatgctgg gcgcggcctc
    15361 gggtgcggtc gccggcctgg tggcgatcac cccggcctgc ggctcggtcg gcccgatggg
    15421 cgcgatcgtg atcggcaccc tctccggctt catctgcctg tggggcgtga atgggctcaa
    15481 gcacatgctc ggtgcggacg acgcgctcga cgtgttcggg gtgcacggcg tgggcggcat
    15541 cgtcggcgcg atcctcaccg gcgtgttcgc cgcgcccagc ctgggcggca ccggcgccga
    15601 ggacttctcg atcgcctcgc aggtgtggat ccagacctgg agcgtgctcg tgaccatcgt
    15661 gtggtcggcc gtggtcgcct tcgtcgccta caaggtcgcg gacctgctgg tcgggctgcg
    15721 cgtgccggaa gacgaggagc gtcagggcct cgacaccacg gcgcacggcg agaccgccta
    15781 ccgctactga gccggcttgc ggaaccgtgc tcctccggtt cccatcccct cgcggcgccc
    15841 ttcggggcgc cgtttttctt tggcgcgccc ccaatggaag cgatcgggcg caacgccctg
    15901 ccggcattcg cgcagcgcaa gcctgccagc ggcaccgaca ccgcaagctt gccgttcgac
    15961 cctccgcacg atgacatcgg gagaatcccc ggggtccaat ccaccccgag gcaacgcccc
    16021 atgcgtcaca agatcgcccc tctccgcctg ctttccctcg ccgcactcgt taccggctg
//
LOCUS       DAOQBS010000067        15662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2852_length_15662_cov_15.068559, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000067 DAOQBS010000000
VERSION     DAOQBS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 15662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15662
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2852_length_15662_cov_15.068559"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /gene="eno"
                     /locus_tag="PKN25_09035"
     CDS             <1..494
                     /gene="eno"
                     /locus_tag="PKN25_09035"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990051.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNV91085.1"
                     /translation="AAGFTDYLETLCDKFPIVSIEDAMAEGDWDGWKILTERLGKRVQ
                     LVGDDLFVTNTRILAQGIEQGVANSILIKINQIGTLTETFAAVEMAKLAGYTAVISHR
                     SGETDDSTIADIAVGLRAMQIKTGSLSRSDRISKYNQLLRIEEDLGDTAAYPGKRAFY
                     NLR"
     gene            593..865
                     /gene="ftsB"
                     /locus_tag="PKN25_09040"
     CDS             593..865
                     /gene="ftsB"
                     /locus_tag="PKN25_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HNV91086.1"
                     /translation="MRWPILILIALVVLLQYPLWLGKGGWLRVWEVDRQLHAQREENL
                     RLEQRNAALAAEVNDLKSGNEAIEERARFELGLTRPGEIYVQIPQR"
     gene            complement(906..1490)
                     /locus_tag="PKN25_09045"
     CDS             complement(906..1490)
                     /locus_tag="PKN25_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNV91087.1"
                     /translation="MSPEPLPRAFLSAPLHAGIGKRRLALLAGVATVAALLAGCGTTP
                     VAGPGSPPRTVAPSGHYFSLEGEHQTQEMVLFALGLLDTGYRFGGRNPDAGLDCSGMV
                     AYIVENISGRKLPHNAAQIADRTRPIEVHELRPGDLVFFNTLERRHSHMGIYIGDGRF
                     VHAPNSRGRVRVERLASRYFAQRIDGARTLLASN"
     gene            complement(1629..2321)
                     /locus_tag="PKN25_09050"
     CDS             complement(1629..2321)
                     /locus_tag="PKN25_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305918.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNV91088.1"
                     /translation="MRTALATLAALAALQSAPALAADTPAPPTVELAAEASGPAANDL
                     AVAVMYAERSGASAAAVAREVNRDIAAALELARAQGAVKVQSGNVSTWPAYGKDGQGR
                     ITAWRMRSELRLESTDTAAMSELVGKLQESLALAQLSMEPAPETRRKAVSDVTVDALR
                     AFEARAKLIADTLGRRYRIAHLSVGEHGLQPPPMPRMRAAAMAAEAAPAPLEGGESRV
                     SVQVGGRIELLD"
     gene            complement(2321..2875)
                     /locus_tag="PKN25_09055"
     CDS             complement(2321..2875)
                     /locus_tag="PKN25_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNV91089.1"
                     /translation="MSIYALGDRRPSFGEGSWIAHNATVIGSVTAGRNVNIWYNVVVR
                     GDNDPITIGDDTNIQDGSVLHNDDGIPLTIGTHVTVGHMAMLHGCTIGDGSLIGINAV
                     ILNNAVVGKQCIVGANTLIPEGKVIPDRSLVVGSPGRVIRTLSDTEVENLVVNAHNYV
                     DNARLYADRLCALEPSSVIAKPEQ"
     gene            3063..3908
                     /gene="rlmJ"
                     /locus_tag="PKN25_09060"
     CDS             3063..3908
                     /gene="rlmJ"
                     /locus_tag="PKN25_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HNV91090.1"
                     /translation="MLSYRHAFHAGNHADVLKHLVLIELLDYYGRKDKPWYYIDTHAG
                     GGCYALDSEQAGKTAEAADGIGRLWGHEDLPPAVAAYVAAVGQFNPQGRLLFYPGSPA
                     LAMTRARAQDRLRLFELHPADFESLQRTFASEQERVQVRRADGFAALKSLLPPPSRRA
                     VVLIDPPYEVKDDYRSVVDTVRDAMRRFPTGTYMVWYPMLARAEARQLPERLAALGAE
                     SWLDVRLAVRDAPRDGIGMFGSGLYVINPPWVLPQRLEAALPWLVEHLGVDGGAGFDL
                     EHHIE"
     gene            4016..4333
                     /locus_tag="PKN25_09065"
     CDS             4016..4333
                     /locus_tag="PKN25_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNV91091.1"
                     /translation="MRQFAAERAWEPFHTPKNLAMALAGEAGEVIEHFQWLTAEQSMA
                     LPPATRDEVALELADVLLYLVRLCDVLGVDLADAARRKMRINAERYPIERAHGRADKH
                     DRL"
     gene            4432..6012
                     /locus_tag="PKN25_09070"
     CDS             4432..6012
                     /locus_tag="PKN25_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNV91092.1"
                     /translation="MDLQHISDIEKAAHQGRGEVFRLGMGVLFIVGVMFYSALSGTGE
                     GSMTLVMAAMIGGYMAMNIGANDVANNVGPAVGSKALTLAGALVIAAIFEAAGALIAG
                     GEVIGTIRSGIIDPDLIKDSDTFVWIMLAALLAGALWLNLATAVGAPVSTTHSIVGAV
                     LGSGMAAAGPSIVDWSTMGEIAASWVISPLLGGIFAAAFLYLVKRSITYQVDMAAAAR
                     RTVPVLVGLMAWTFVSYLILKGLNRVWKVDFLPAVLYGLIFGALVFVLVRKALHGSSG
                     IVANTKASVNRLFTVPLIFAAALLSFAHGSNDVANAVGPLAAIVDVVSSGGVVHKDAA
                     IPTWVMMVGAIGISLGLALFGPKVIRTVGSEITELDQMRAYCIAMAATLTVIVASQLG
                     LPVSSTHIAVGGVFGVGFLREYLKSNYDRMVAEIKAHHPEGDLAAIEAFIARFEKASI
                     EEKGQMLADLKQRSKNADDPAHFSKGERKGLKKVYRRELVKRSQLMRIAAAWVITVPA
                     SALMAAVLFFMIRGMMLP"
     gene            6053..6412
                     /locus_tag="PKN25_09075"
     CDS             6053..6412
                     /locus_tag="PKN25_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HNV91093.1"
                     /translation="MSFVELLVLLALGAVVWFWFDSLKAREIGIEAARRACTREGVQF
                     LDETVVGQGLRLARDDNGRVLLRRGYAFEYSVGGDDRNAGSVVLEGREVVLVDVSAHR
                     LARRAVVIDLHPRDPSA"
     gene            complement(6409..7062)
                     /gene="folE"
                     /locus_tag="PKN25_09080"
     CDS             complement(6409..7062)
                     /gene="folE"
                     /locus_tag="PKN25_09080"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNV91094.1"
                     /translation="MSPPAHDPCGHDHPHAPRPAGVGIPRSGAFDAPAFEKAVGDLLR
                     ACGIAPDNAHTGRTAQRVRELWQRRLLGGYELDPAEVLGDGFEDTRHDMVVIRGIAVH
                     GVCPHHLVPFRGVAHVAYLPGGRLHGFGRIARLVDAIGHRFTYQEWMTRDIADALMRH
                     GHAAGAACVIEAEQLCLLLGEDRRGDERVITQAFAGEFERSEQARNEFLRAIEERRR"
     gene            complement(7122..7718)
                     /locus_tag="PKN25_09085"
     CDS             complement(7122..7718)
                     /locus_tag="PKN25_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91095.1"
                     /translation="MIDMDAARAMLGRQAILLPDAVPSHPLLDGKTEIGRGEYSIVID
                     KGDGEHVYKIVSSPADYFLYTADDRPRGPHFPIVFADHGVIGRAQSGYPFHLIEMERL
                     YPLAEGSPAAELSRRLIECYWSACAQWSRLGADMGRIALYHLTVEPMKVGDSLQEALR
                     ALSDFVEEYQVLPDILNANNLMMRADGTLVFSDPVFIA"
     gene            complement(7733..8512)
                     /gene="fabI"
                     /locus_tag="PKN25_09090"
     CDS             complement(7733..8512)
                     /gene="fabI"
                     /locus_tag="PKN25_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HNV91096.1"
                     /translation="MTPIVNLEGKVGLITGIANEKSISTGVAEACAQAGATLIITYQN
                     EKTRAFIEPVIHRLGVEDVHLLDVTRPETMDAAFGAIEARHGKLDFAIHSMAFAKRDD
                     LHGRVVDTSADGFALAMDVSTHSFVRLAKKAEPLLEKAGGGALVTMTYLGSEKVIPNY
                     GVMGLCKAALEAATRYMAAELGAKGIRVNAVSPGPLMTRAASGIAGFDGLMAAAVERS
                     PMHALVTPEDIGALTAFLVSDAGRLISGGVHFVDAGYNIMA"
     gene            8700..9209
                     /locus_tag="PKN25_09095"
     CDS             8700..9209
                     /locus_tag="PKN25_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HNV91097.1"
                     /translation="MTRNAYIFMDFDGVTHPWGEVEDFRCLVHIEAVVREFPEARIVI
                     ASDWRMLFSLPKLVARFSEDIRPQIAGATPHLLPKTGAELHGLREREAMAWLRQHGAD
                     PGEAAWCALDDAPGNWLSRSRLVLTDFKRGFTSEDAVALRRMLQGFRDGVRNQVGQVA
                     HSLWARDSA"
     gene            9224..9994
                     /locus_tag="PKN25_09100"
     CDS             9224..9994
                     /locus_tag="PKN25_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91098.1"
                     /translation="MNPAEQTLPPDPLAHRVDASTQRQAARLAEESFARLFRLSVAEG
                     DAARLKGVEQLRVDLADWVSAAADPEAQALRLALLLSGMDQWGMAWSRAFGLVAIPAL
                     TELIGALRTGLDETAEARFLRHFEGISAVEENAIDFKVELRRGLHLALWHASIATEHR
                     DEAMRLAGELGSQLLALARSMPVAGWRLVADALAFIQIRCLAEGLAAEGVAQEATQAL
                     FGALARELPAPQRDLVMAHSARAVMAWQQAGRASPQVH"
     gene            9994..10530
                     /locus_tag="PKN25_09105"
     CDS             9994..10530
                     /locus_tag="PKN25_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNV91099.1"
                     /translation="MALKATVFKADIQVADMDRHHYADYSLTLARHPSETDERMMVRL
                     LAFALFADPALAFGKGLCVDDEPDLWQKDLTGVIERWIDVGQPDDKWVRKACGRAREV
                     VVLAYGRALEVWWSGVRGRLERHGNLKVFELEREASAELGRLAERTMRLQFTIQDGHV
                     LVTNGRETVSLEPRKLTA"
     gene            complement(10577..>11350)
                     /locus_tag="PKN25_09110"
     CDS             complement(10577..>11350)
                     /locus_tag="PKN25_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV91100.1"
                     /translation="SSLTYAPMLAHLSAHFDLFLSDVQGHGDSDHGSRFRGWNHAAEM
                     AAEAWLAHAPRFGTVPVHAAGHSFGSVLTCLMLAHHPHAFSRGVLLDPVLFTPAMIGM
                     MALSDLVGLASRNKLAAKARTRRHHWPDRAAAHAGLHGRGVFRGWSEDSFQAYIDHAL
                     RDVPGGGVELKCRPTREAEIFASYPRRLWSSLARIEQPVLALHGRDTFAFARRSVTRW
                     AAGKANVQCVQVEGGHCFMLEHPQATAERVAAFLRDSPR"
     assembly_gap    11351..11450
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11451..14371)
                     /locus_tag="PKN25_09115"
     CDS             complement(<11451..14371)
                     /locus_tag="PKN25_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV91101.1"
                     /translation="MRRLLRTVVLLVCLSLGSAALHARTDLDFGVYSARPKALVEASW
                     QPFVDYLNQHLDGVEVRLHALDDAGLQRALAAGEIDLLLTNPVHFIELRASNPLSGAI
                     ATVVADAGGQALSEFGGVILVRAGDASLKTLADLRGRRVGTTHANFLATYPAQALEMK
                     AAGIDPGSLRMFTLRQTQDSVVEAVLDGRVDAGFVRTGLFEALVAEGRDLSGLRVLHP
                     LQHPGFPLRSSTRLYPEWPLVALRQADPALMRKVAALALGLETADPAARAAGIHGFTI
                     PADYARVEEMLRELRLPPFDATPPFTWNDVWARYHETIFVLGLATLAIVLLLVRLAQR
                     NLALSQANAAARRLAAEIELDRHHLRNVIEATQAGSWEWNLETGEWKMDARWAAMLGL
                     AAEDCDGEGRACWRKRVHPADLAEAERELDRHIRGETAFYEHDLRLRHCDGRWIWVYD
                     RALAVRRDADGRALLLTGAQIDIDRRKENEERLRLAASVFSSSYEAILITDVDNRIVD
                     VNPAFCRITGYSREESLGRDPGMLASGRQGREFYRAMWQAIEEHDHWQGELWNRRKDG
                     SEFAEVLSISRVRDARGRVIHHLAMFSDISRLKKHEEELNRIAYFDPLTGAPNRRLLD
                     DRLRQAIAHSRRTGKPLAVCVIDLDGFKPVNDQLGHEAGDQVLIGIVDRLGAILRASD
                     TVARLGGDEFVLLLEDIEGERVLERVLEAIRTPIALASQQVCVSASIGVTLFPEDDAD
                     PDTLLRHADQAMYRAKQRGRNCIQFFDASVEEQQRVRKERLERLDQALRRQEFVLHFQ
                     PQVDMVDGRPLGMEALLRWQHPERGLLAPGEFLPDLEGTELETRLGQWVVDHALGALE
                     RCRAAGLDIGVSVNVGPGPLMQAGFVASVRSALERHPDIPPERLELEILESTALDDMV
                     LAMDVLEACHRLGVRIALDDFGTGYASLRHFRQLPFDRLKIDRSFVLDMLEDGEARAI
                     VG"
     gene            complement(14628..>15662)
                     /locus_tag="PKN25_09120"
     CDS             complement(14628..>15662)
                     /locus_tag="PKN25_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HNV91102.1"
                     /translation="PYDTEAYVHRIGRTGRAGREGVAILFVAPRETRMLKMIERATRQ
                     PITPIALPSGEEVTNLRVAQFKRQVAETIAGAELGFFMGVVNDLIDENELEIHEVAAA
                     LALLAQRERPLQVEQSGRGWDIATTTPSEGRAPRAEHFTTGAARERTPRPNRDEILAR
                     RRSFSEGALVRYRIEVGRAHGASPKEIVGAIANEGGIEGRFIGQIHLFDEFSTVELPA
                     NLPEDLLATLKRTRVRQMPLSIRELSAAEAATVPERRRPARPERGTDGSHAQDGERRP
                     RDFGGARPNQPGAGYAGKPGGGGFRGHDERRPHDERRAPATDRKAGGHSAPVKRQGER
                     AGKPFRRRED"
BASE COUNT         2246 a         5232 c         5638 g         2446 t
ORIGIN      
        1 ccgccgcggg cttcaccgac tacctcgaga ccctgtgcga caagttcccg atcgtctcga
       61 tcgaggacgc catggccgag ggcgactggg acggctggaa gatcctcacc gagcgcctgg
      121 gcaagcgcgt gcagctggtg ggcgacgacc tcttcgtcac caacaccagg atcctcgcgc
      181 agggcatcga gcagggcgtg gccaactcga tcctcatcaa gatcaatcag atcggcacgc
      241 tcaccgagac cttcgcggca gtcgagatgg ccaagctcgc cggctacacc gcggtgattt
      301 cgcaccgctc gggcgagacc gacgattcga ccatcgccga catcgcggtg ggcctgcgcg
      361 ccatgcagat caagaccggc tcgctgagcc gctcggaccg catctcgaag tacaaccagc
      421 tgctgcgcat cgaggaagac ctcggcgaca ccgccgccta tccgggcaag cgcgcgttct
      481 acaacctgcg ctgagcgtcc tgcaacgctg attcgcagcg tctcgaaggc cggcgggcga
      541 ctcgccggcc ttcgtcgtga atgcgccagt atccatctcc atacccgtct ccatgcgctg
      601 gccgatcctc atcctgatcg cactcgtcgt gctgttgcag tacccgctct ggctggggaa
      661 agggggctgg ctgcgcgtct gggaggtcga tcgccagctg catgcgcagc gcgaggagaa
      721 cctgcgcctc gagcagcgca acgcggcgct cgcggcggag gtgaacgatc tcaagtcggg
      781 caacgaggcg atcgaggagc gcgcgcgttt cgaactcggg ctcacccggc cgggcgagat
      841 ctacgtgcag attccgcagc gctgaaccct ggtcgcgggt atcgccgccg ttccggcgac
      901 agtcctcagt tgctcgcgag cagcgtgcgc gcgccgtcga tgcgctgcgc gaaatagcgg
      961 ctggcgagac gttcgacccg cacccggcca cggctgttgg gcgcgtgcac gaagcgcccg
     1021 tcgccgatgt agatccccat gtgcgaatgc cggcgctcca gcgtgttgaa gaacaccagg
     1081 tcccccgggc gcagttcgtg cacctcgatc gggcgcgtgc gatcggcgat ctgcgcggcg
     1141 ttgtgcggca gcttgcggcc gctgatgttc tccacgatgt aggccaccat gccgctgcaa
     1201 tcgaggcccg cgtccggatt gcgcccgccg aagcggtagc cggtatcgag caggccgagc
     1261 gcgaagagca ccatttcctg cgtctggtgc tcaccctcga gagagaaata gtgcccgctc
     1321 ggcgccaccg tgcgcggtgg cgagccgggg ccggcgaccg gggtggtgcc gcagcccgcc
     1381 agcagtgcgg ccacggtggc cacgccggca agcagcgcca ggcgccgctt gccgatgccg
     1441 gcgtgcagag gggcggaaag gaaggcgcga gggaggggct cgggactcat ggcgcgatcc
     1501 ggtgcgaaaa ggatttctgc cagagtatgt caggacccgc ccaggccggc aaggtctgct
     1561 ttcagatcag gggtttggag aggttttctc gaagaaggct gaagtcgggc ccgcgcgggg
     1621 cgcggtcttc agtcgagcag ttcgatgcgt ccgccgacct gcaccgacac ccggctctcg
     1681 ccgccctcga gtggcgccgg ggcggcctcc gcggccatcg ccgccgcgcg catgcgcggc
     1741 atcggcggag gctgcaggcc gtgctcgccg accgacaggt gggcgatgcg gtagcgccga
     1801 ccgagggtgt cggcgatcag tttcgcgcgc gcctcgaagg cgcgcagggc gtcgacggtg
     1861 acgtcggaga cggccttcct gcgcgtctcg ggggctggtt ccatgctcag ctgggcgagc
     1921 gccaggctct cctggagctt gccgacgagc tccgacatcg ccgccgtgtc ggtgctctcg
     1981 aggcggagct cggaacgcat ccgccaggcg gtgatacggc cctggccgtc ctttccgtat
     2041 gccggccagg tcgagacgtt tccgctctgc accttgacgg caccctgcgc gcgcgcgagt
     2101 tcgagcgccg cggcgatgtc gcggttcacc tcgcgtgcca ccgcggccgc gctcgcgccg
     2161 ctgcgctctg catacatcac ggcgaccgcg aggtcgttgg cggccgggcc gctggcctcg
     2221 gccgcgagct ccacggtggg cggggcggga gtgtcggcgg caagcgccgg cgccgactgc
     2281 agcgccgcga gcgcggcgag ggtggcgagc gcggtgcgca tcactgttcc ggcttcgcga
     2341 tcacgctgga cggctccagc gcgcacaggc ggtcggcgta gaggcgggcg ttgtccacgt
     2401 agttgtgggc gttgaccacc aggttctcga cctcggtgtc gctcagcgtg cggatcaccc
     2461 ggccaggaga accgaccacc agcgagcggt cggggatcac cttgccctcg gggatcaggg
     2521 tgttggcgcc gacgatgcac tgcttgccga ccaccgcgtt gttgaggatc accgcgttga
     2581 tgccgatcag gctgccgtcg ccgatcgtgc agccgtgcag catcgccatg tggcccacgg
     2641 tgacgtgcgt gccgatcgtc agcgggatgc cgtcgtcgtt gtgcaggaca gagccgtcct
     2701 ggatgttggt atcgtcgccg atcgtgatcg gatcgttgtc gccccgtacc acgacgttgt
     2761 accagatgtt gacgttgcgg cccgcagtga ccgatccgat gaccgtcgcg ttgtgggcga
     2821 tccagcttcc ctcgccgaag ctcggccggc ggtcgcccag ggcgtagatg ctcattttcg
     2881 ttctcgctgt cgatgtctcg cgccgatgct gcgcagcgcc aggggggcag gcatcataac
     2941 aagcgcctcg cggcctgcaa acagcgccca tgccgtgaat gtcgccgatc cggcgggttt
     3001 ttcgcgattt tgagccccct gccggcgggt gataggatgc gcccgccctt caggaagtcc
     3061 acatgctcag ttaccgtcac gcctttcacg ccggcaatca cgccgatgtc ctcaagcacc
     3121 tcgtcctgat cgagctgctc gactactacg gtcgcaagga caagccctgg tactacatcg
     3181 acacccacgc ggggggcggc tgctatgcgc tcgacagcga gcaggcgggc aagaccgccg
     3241 aggcggcgga cggcatcggc cggctgtggg ggcacgagga cctgccgcca gcagtcgccg
     3301 cctatgtcgc ggcggtcggg cagttcaatc cgcagggcag gctgttgttc tacccgggct
     3361 cgccggcgct ggccatgacg cgtgcgcgcg cccaggatcg cctgcgcctg ttcgagctgc
     3421 atccggccga cttcgaatcc ctgcagcgca ccttcgccag cgaacaggag cgcgtgcagg
     3481 tgcgccgcgc cgacggcttc gccgcgctca agagcctgct gccgccgccc tcgcggcggg
     3541 cggtggtgct gatcgatccg ccctacgagg tcaaggacga ctaccgcagc gtggtggaca
     3601 cggtgcgtga cgcgatgcgg cgctttccca ccgggaccta catggtctgg tatccgatgc
     3661 tcgcccgcgc cgaggcccgc cagttgcccg agcgcctcgc cgcgctcggc gccgagagct
     3721 ggctggacgt gcgcctggcg gtgcgcgacg ccccgcgcga cggcatcggg atgttcggca
     3781 gcggccttta cgtgatcaac cctccctggg tgttgccgca gcggctcgag gccgcgctgc
     3841 cctggctcgt cgagcacctg ggggtcgatg gcggtgcggg cttcgacctc gagcaccaca
     3901 tcgaatgagt ccgtcccgga atgccgaggg tgggcccgcc ggcccgacgg tcgcggaggg
     3961 cgctccgagt gctgcaggcc cgtcgcccga cgcgctccgc gagctgcgcg acgcgatgcg
     4021 gcaattcgcc gccgagcgcg cctgggagcc cttccatacg cccaagaacc tcgcgatggc
     4081 gctcgccggc gaggcgggcg aggtgatcga gcatttccag tggctcaccg ccgagcagtc
     4141 gatggcgctg ccgccggcga cgcgggacga ggtcgctctc gagctcgccg acgtgctgct
     4201 gtacctcgtg cgcctatgcg acgtgctcgg tgtcgacctc gccgatgccg cacggcgaaa
     4261 gatgcgcatc aatgcggagc gctaccccat cgagcgcgcg cacggccgcg cggacaagca
     4321 cgaccgtctg tgagggtgca agcgcggcgt tgcggcggag tcttcattca cgttgggccg
     4381 cggcaagatt tctgaaacaa tatcgtcttt cttgtaacac ggaattccat cgtggacttg
     4441 cagcacatca gcgacatcga gaaggcagcc catcagggtc ggggcgaggt cttccgcctc
     4501 ggcatgggcg tgctcttcat cgtcggggtc atgttctact cggcgctcag cggcacgggc
     4561 gagggcagca tgaccctggt catggcggcg atgatcggcg gctacatggc gatgaacatc
     4621 ggcgccaacg acgtcgccaa caacgtcggt ccggcggtgg gctccaaggc gctcaccctc
     4681 gcaggtgcgc tcgtcatcgc ggcgatcttc gaggccgccg gtgcgctgat cgccggcggc
     4741 gaggtgatcg gcacgatccg cagcggcatc atcgatcccg acctcatcaa ggattccgac
     4801 accttcgtgt ggatcatgct ggccgcgctg ctcgccggcg ccttgtggct gaacctggcg
     4861 accgcggtcg gggcgccggt gtcgaccacg cactcgatcg tgggcgcggt gctcggctcc
     4921 ggcatggccg ccgccggccc ctcgatcgtc gattggtcca ccatgggcga gatcgcggcg
     4981 agctgggtga tctcgccgct gctcggcggg atcttcgccg ccgccttcct gtatctcgtc
     5041 aagcgcagca tcacctacca ggtggacatg gccgcggccg cacgccgcac cgtgccggta
     5101 ctggtcggcc tgatggcgtg gaccttcgtc agctacctga tcctgaaggg gctcaaccgg
     5161 gtctggaagg tggacttcct tcccgccgtg ctgtacggcc tgattttcgg cgcgctggtg
     5221 ttcgtcctgg tgcgcaaggc cctgcacggc agcagcggca tcgtcgccaa caccaaggcg
     5281 agcgtgaatc ggctcttcac cgtgccgctg atcttcgccg ccgcgctgct cagcttcgcg
     5341 cacggctcca acgacgtcgc caacgcggtc ggcccgctgg ccgcgatcgt cgatgtggtc
     5401 agttccggcg gcgtggtgca caaggacgcg gcgatcccca cctgggtcat gatggtcggc
     5461 gcgatcggga tctcgctcgg gctggcgttg ttcgggccga aggtgatccg cacggtcggt
     5521 tccgagatca ccgagctcga ccagatgcgc gcctattgca tcgcgatggc ggcgaccttg
     5581 accgtgatcg tcgccagcca gctcggcctg ccggtgagct ccacgcacat cgcggtcggc
     5641 ggggtgttcg gcgtcggttt cctgcgcgaa tacctcaaga gcaactacga ccgcatggtg
     5701 gccgagatca aggcccacca ccccgagggc gacctcgccg cgatcgaggc cttcatcgcg
     5761 cgtttcgaga aggcctcgat cgaggagaag gggcagatgc tcgccgacct caagcagcgc
     5821 tccaagaacg cggacgatcc ggcgcacttc tccaagggcg agcgcaaggg actcaagaag
     5881 gtctatcgcc gcgagctggt caagcgctcg cagctgatgc gcatcgccgc cgcctgggtc
     5941 atcaccgtgc ccgcttctgc gctgatggcc gcagtgctgt tcttcatgat ccgcgggatg
     6001 atgctgccct gatcaccctg ttcgtcatga tcgcgatgcc gggggccgcg gcgtgagctt
     6061 cgtcgagctg ctcgtcctgc tcgccctcgg ggccgtggtg tggttctggt tcgacagcct
     6121 gaaggcgcgc gagatcggca tcgaggccgc gcgccgcgcc tgcacgcgcg agggcgtgca
     6181 gttcctcgac gagaccgtgg tggggcaggg cctgcgcctg gcgcgcgacg acaacggtcg
     6241 cgtgctgctg cggcggggct atgccttcga atactcggtg ggcggcgacg accgcaatgc
     6301 cggcagcgtg gtgctcgaag gccgcgaggt ggtgctggtg gacgtctccg cgcatcggct
     6361 cgcacgtcgc gcggtggtca tcgacctgca cccgcgcgac ccgtcggcct agcgccggcg
     6421 ctcctcgatc gcgcgcagga actcgttgcg cgcctgctcc gaacgttcga actcgccggc
     6481 gaaggcttgg gtgatcacgc gttcgtcgcc gcggcggtcc tcgccgagca gcaggcagag
     6541 ctgttcggcc tcgatcacgc aggccgcgcc cgcggcgtgg ccgtggcgca tcagcgcgtc
     6601 ggcgatgtcg cgcgtcatcc actcctggta ggtgaagcga tggccgatcg cgtccaccag
     6661 gcgggcgatg cggccaaagc cgtgcaggcg gccgcctggc aggtaggcga cgtgggcgac
     6721 gccacggaaa gggacgaggt gatgcggaca cacgccatgg accgcgatgc cgcgaatcac
     6781 caccatgtcg tggcgcgtat cctcgaagcc gtcaccgagc acctcggcgg ggtcgagctc
     6841 gtagcccccg agcaagcgcc gctgccagag ctcgcgcacg cgctgcgcgg tgcgtccggt
     6901 atgggcgttg tcgggggcga tgccgcacgc acgcagcagg tcgccgacgg ccttctcgaa
     6961 ggcgggcgcg tcgaaggcgc cgctgcgcgg aatgccgacg ccggcggggc ggggagcgtg
     7021 ggggtggtcg tgaccgcagg ggtcgtgcgc gggagggctc atggtggcgt ccggtgcggt
     7081 gccctgcggc acccgagccg gcatgatacc cgcaagcccg ttcaggcgat gaacaccggg
     7141 tcggagaaca ccagcgtgcc gtcggcgcgc atcatcaggt tgttggcgtt gaggatgtcg
     7201 ggcaggacct gatattcctc gacgaagtcg gacagggccc gcagggcttc ctgcaggctg
     7261 tcgccgacct tcatcggctc gaccgtgagg tggtagagcg cgatccgccc catgtccgca
     7321 ccgagccggc tccactgcgc gcacgccgac cagtagcact cgatcaggcg acgcgagagt
     7381 tcggccgcgg gggagccctc ggcgagcgga tagaggcgct ccatctcgat cagatggaag
     7441 ggatagcccg actgtgcgcg gccgatcacc ccatgatcgg cgaatacgat ggggaaatgc
     7501 gggccgcgcg ggcggtcgtc ggcggtgtag aggaagtagt cggcagggga gctgacgatc
     7561 ttgtagacat gctccccgtc gcccttgtcg atcacgatcg agtactcgcc gcgaccgatc
     7621 tcggtcttgc cgtcgagcag cggatgcgac ggcacggcgt cgggcagcag gatcgcctgc
     7681 cgtccgagca tcgcgcgcgc cgcatccatg tcaatcatga tctcctcgct gctcacgcca
     7741 tgatgttata gccggcgtcg acgaaatgca ccccgccgct gatcagtcgg ccggcgtccg
     7801 agaccaggaa ggcggtcagc gcgccgatgt cctcgggggt gaccagggca tgcatcggcg
     7861 agcgctcgac cgcggccgcc atgaggccgt cgaagcccgc gatcccggag gcggcgcgcg
     7921 tcatcagcgg gccgggggag accgcattga cgcggatgcc cttggcgccg agctcggccg
     7981 ccatgtagcg ggtggcggcc tcgagcgcgg ccttgcacag gcccatcacg ccgtagttgg
     8041 ggatgacctt ctcggagccg aggtaggtca tcgtcaccag ggcaccgccg ccggccttct
     8101 cgagcagcgg ctcggccttc ttggccaggc gcacgaagga gtgggtggac acgtccatcg
     8161 ccagcgcgaa gccgtcggcc gaggtgtcga ccacgcggcc gtgcaggtcg tcgcgcttgg
     8221 cgaaggccat gctgtggatg gcgaagtcga gcttgccgtg gcgcgcctcg atcgcgccaa
     8281 aggcggcgtc catggtttcg gggcgggtga cgtcgagcag gtgaacgtcc tcgacgccga
     8341 ggcggtggat caccggctcg atgaaggcgc gcgtcttctc gttctgatag gtgatgatca
     8401 gcgtcgcgcc ggcctgggcg caggcctcgg cgacgccggt ggagatcgac ttctcgttgg
     8461 cgatgccggt gatgaggccg accttgccct ccagattgac gatgggggtc atgatggatt
     8521 ccttttctcg ggtgagtgtt gttgcggcgc aacggcagcg agcataagcc ggcctgtgcg
     8581 acgcgaatgt gatggttcgt gaaatcggat acgcggtggt atcggtgccg gtacagtata
     8641 ttgcgcattg cagcatccac aaattgccgg ccgcggcatt cttcgccgga gcagggaaca
     8701 tgacacgcaa cgcatacatc ttcatggact tcgacggcgt cacccacccc tggggcgagg
     8761 tcgaggattt ccgttgtctc gtgcacatcg aggcggtggt gagggagttc cccgaagcgc
     8821 gcatcgtgat cgcctccgac tggcgcatgc tgttctcgct gcccaagctg gtcgcccgct
     8881 tttccgagga catccgcccg cagatcgccg gcgccacgcc gcacctgctg cccaagacgg
     8941 gcgccgagct gcacggcctg cgcgagcgcg aggcgatggc ctggctgcgc cagcacggcg
     9001 ccgatccggg cgaggcggcg tggtgcgcgc tcgacgacgc gccgggcaac tggctgtcgc
     9061 gttcgcgact ggtgctgacc gacttcaagc gtggcttcac ctccgaggat gcggtcgccc
     9121 tgcgccgcat gctgcagggc ttccgcgacg gggtgcgcaa ccaggtcggc caggtcgcgc
     9181 actcgctgtg ggcgcgcgac agcgcctgat ggggggagac gccatgaacc ccgccgagca
     9241 gaccctgccg cccgacccgc ttgcgcaccg agtcgacgcc tcgacccaac gccaggcggc
     9301 gcggctggcc gaagagagct tcgcgcgcct gttccggctc agcgtcgccg aaggcgatgc
     9361 tgcgcggctg aagggcgtgg agcagttgcg cgtcgacctc gccgactggg tgtccgcggc
     9421 ggccgacccc gaggcacagg cgctgcgcct cgccttgctg ctgtccggga tggaccagtg
     9481 ggggatggcc tggagccggg ccttcggcct ggtggcgatc cccgcgctga ccgagctcat
     9541 cggtgcgctg cgcactggtc tggatgaaac cgccgaggcg cgcttcctgc gccacttcga
     9601 agggatctcc gccgtcgagg agaatgccat cgacttcaag gtcgagctgc gccgtgggct
     9661 gcacctggcg ctgtggcatg cctcgatcgc caccgagcat cgcgacgagg ccatgcgcct
     9721 cgccggcgag ctcggcagcc agctgctcgc gctcgcgcgc agcatgccgg ttgcgggctg
     9781 gcggctggtg gccgacgcgc tcgccttcat ccagatccgc tgcctggccg aagggctcgc
     9841 tgccgagggc gtggcccagg aggccacaca ggccttgttc ggcgccttgg cgcgcgagct
     9901 gccggcgccg cagcgcgacc tcgtgatggc gcattccgcg cgcgcggtga tggcctggca
     9961 gcaggccggc cgcgcttcgc cgcaggtgca ctgatggcgc tcaaggccac ggtcttcaag
    10021 gccgacatcc aggtcgccga catggatcgg caccactacg ccgactattc gctcaccctc
    10081 gcgcgtcacc cttccgagac cgacgagcgc atgatggtgc gcctgctcgc cttcgcgctc
    10141 ttcgccgacc cggcgctcgc cttcggcaag gggctatgcg tcgatgacga gcccgacctg
    10201 tggcagaagg acctcaccgg cgtcatcgag cgctggatcg acgtcggcca gcccgatgac
    10261 aagtgggtgc gcaaggcctg cgggcgcgcg cgcgaggtgg tggtgctggc ctacgggcgg
    10321 gcgctggagg tgtggtggag cggggtgcgc ggccgcctcg agcgccacgg caacctgaag
    10381 gtgttcgagc tcgaacgcga ggccagcgcc gaactcggcc gcctcgccga gcgcacgatg
    10441 cgcctgcaat tcaccatcca ggacggccac gtgttggtaa ccaacgggcg cgagaccgtg
    10501 tcgctcgagc cgcgcaagct cactgcctga accccggtgc gcggcccgac agcccgcggc
    10561 gcggccgccc gggccctcag cgcgggctgt cgcgcaggaa tgcggccacc ctttcggcgg
    10621 tcgcctgcgg atgctcgagc atgaagcagt gtccgccctc cacctgcacg cattgcacgt
    10681 tggccttgcc tgccgcccag cgcgtgaccg agcgccgcgc gaaggcgaag gtgtcgcgcc
    10741 cgtgcagcgc gagcaccggc tgctcgatgc gggcaagcga ggaccacagc cggcgcgggt
    10801 aggacgcgaa gatctcggcc tcgcgggtgg gccggcactt gagctccacg ccaccgcccg
    10861 gcacgtcgcg cagcgcatgg tcgatatagg cctggaagga gtcctcgctc cagccgcgga
    10921 acaccccgcg cccgtgcagc cccgcatgcg cggcagcgcg gtccggccag tggtggcgtc
    10981 gcgtgcgtgc cttggcggcg agcttgttgc gcgaggccag cccgacgagg tcggagagcg
    11041 ccatcatgcc gatcatcgcc ggggtgaaca gcaccgggtc gagcagcaca ccacggctga
    11101 aggcgtgcgg atgatgcgcc agcatcaggc aggtgagcac gctgccgaag ctgtggccgg
    11161 cggcatgcac gggtaccgtg ccgaaacgcg gcgcgtgcgc gagccaggcc tcggcggcca
    11221 tctcggcagc atggttccag ccgcggaagc ggctgccgtg gtcgctgtcg ccatgaccct
    11281 gcacgtcgga caggaagagg tcgaaatgcg cgctcaggtg cgcgagcatg ggcgcatagg
    11341 tgagcgagct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgacgatcg
    11461 cgcgcgcctc gccgtcctcc agcatgtcga gcacgaagct gcggtcgatc ttgaggcggt
    11521 cgaagggcag ctggcggaag tggcgcagcg aggcgtagcc ggtgccgaag tcgtcgagcg
    11581 cgatgcgtac gccgaggcga tggcaggcct cgagcacgtc catcgccagc accatgtcgt
    11641 cgagcgcagt ggattcgagg atctcgagct ccaggcgctc gggcgggatg tcggggtggc
    11701 gctccagcgc gctgcgcacg ctggcgacga agcccgcctg catcagcgga ccgggaccga
    11761 cgttgacgct taccccgatg tccagcccgg ccgcgcggca gcgctcgagg gcgccgagcg
    11821 cgtggtcgac cacccactga ccgagccggg tctccagctc ggtgccttcg aggtcgggca
    11881 ggaactcgcc cggcgccagc aggccgcgct cggggtgctg ccagcgcagc agggcctcca
    11941 tgccgagtgg ccgcccgtcg accatgtcga cctggggctg gaagtgcagc acgaactcct
    12001 gccggcgcaa ggcctggtcg aggcgctcga ggcgttcctt gcgcacgcgc tgctgttcct
    12061 cgacgctggc gtcgaagaac tggatgcagt tgcgtccgcg ctgcttggcg cggtacatcg
    12121 cctggtcggc gtggcgcagc agggtgtcgg ggtcggcgtc gtcctcgggg aagagggtga
    12181 cgccgatgct cgcggacacg catacctgct ggctggcgag cgcaatcggc gtgcggatgg
    12241 cttcgagcac gcgctcgagc acgcgctcgc cttcgatgtc ctcgagcagc agcacgaact
    12301 cgtcgccgcc gaggcgggcc acggtgtcgc tggcgcgcag gatcgcgccg agccggtcga
    12361 cgatgccgat cagcacctgg tcgccggcct cgtggccgag ctgatcgttc accggcttga
    12421 agccgtcgag gtcgatcacg cagaccgcga gcggcttccc tgtgcgccga gagtgtgcga
    12481 tcgcctggcg caggcggtcg tcgagcaggc ggcggttggg cgcgccggtg agcgggtcga
    12541 agtaggcgat gcggttgagc tcctcctcat gcttcttgag ccggctgatg tcggagaaca
    12601 tcgccagatg gtggatcacc cgaccgcgtg cgtcgcgcac gcggctgatc gagagcacct
    12661 cggcgaactc gctgccgtcc ttgcgccggt tccacagttc gccctgccag tggtcgtgct
    12721 cttcgatcgc ctgccacatc gcacggtaga actcgcgccc ctggcggccc gaggcgagca
    12781 tgccggggtc gcggccgagg ctctcctcgc ggctgtagcc ggtgatgcgg cagaaggccg
    12841 ggttgacgtc gacgatgcgg ttgtcgacgt cggtgatcag gatcgcctcg tagctcgacg
    12901 agaacacgct cgcggccagg cgcaggcgct cctcgttctc cttgcgccga tcgatgtcga
    12961 tctgcgcgcc ggtcagcagc agcgcgcgcc cgtccgcgtc gcggcgcacg gcgagcgcgc
    13021 ggtcatacac ccagatccag cgtccgtcgc aatggcgcag gcgcaggtcg tgctcgtaga
    13081 aggcggtctc gccgcggatg tggcgatcga gctcgcgctc ggcctcggcg aggtcggcgg
    13141 gatgcacgcg cttgcgccag cacgcgcgcc cttcgccgtc gcagtcctcc gccgccagcc
    13201 cgagcatcgc cgcccagcgt gcgtccatct tccactcgcc ggtctccaga ttccattccc
    13261 agctgccggc ctgggtggcc tcgatcacgt tgcgcaggtg gtggcgatcg agttcgatct
    13321 ctgctgccag gcggcgcgcg gcggcgttgg cctgcgacag cgccaggttg cgctgcgcca
    13381 gacgcaccag cagcagcacg atcgccaggg tcgcgaggcc gagcacgaag atcgtctcgt
    13441 ggtagcgcgc ccacacgtcg ttccaggtaa agggtggagt ggcgtcgaag ggcggcaggc
    13501 gcagttcgcg cagcatctcc tcgacgcggg cgtaatcggc ggggatggtg aagccgtgga
    13561 tgcctgcggc gcgcgcggcc gggtcggcgg tttcgaggcc gagcgcgagt gccgccacct
    13621 tgcgcatcag ggccgggtcg gcctgacgaa gggcgacgag gggccattcg gggtagaggc
    13681 gtgtggaact gcgcagcggg aagcccggat gctgcagggg atgcagcaca cgcaagccgg
    13741 agaggtcgcg cccctcggcg acgagcgcct cgaagaggcc ggtgcggacg aagccggcgt
    13801 cgacgcggcc gtcgagtacc gcttcgacga cgctgtcctg ggtctgccgc agcgtgaaca
    13861 tgcgcagcga gccggggtcg atgccggcgg ccttcatctc gagtgcctgc gccggatagg
    13921 tggcgaggaa gttggcatgg gtggtgccga cgcgcctgcc ccgcaggtcg gcgagggtct
    13981 tcaggctggc gtccccggcg cgcaccagga tcacgccgcc gaactcgctc agggcctggc
    14041 cgccggcatc ggcgaccacg gtggcgatgg caccggagag cggattggat gcgcgcagct
    14101 cgatgaaatg caccgggttg gtcagcagca gatcgatctc gccggcggcg agggcgcgct
    14161 gcagtccggc gtcgtcgagc gcgtgcaggc gtacctcgac gccgtccagg tgctggttca
    14221 ggtagtccac gaagggctgc cacgaggcct cgacgagcgc cttcgggcgc gccgaataga
    14281 cgccgaaatc gagatccgtc cgggcgtgca gagccgcgct gccgaggctc aggcagacca
    14341 gcaggacgac ggtgcggagg agtcgtcgca tcagcacggg atggccaccg agtccgtcgc
    14401 gcgatgcgcc agcaggctcg acgtgcgcgc acggcagcgg cgcgcgggcg agtcttcggg
    14461 ctggcggaga gcgtcacgac acatcgacgg gagagctatg ggacaactgc ccattctagg
    14521 tctttcggga tgcctgcggc aggcgattcg gcggtgccgt gacgcagttc gcgcagcagc
    14581 cgggctgcgt gccccggggc aggggcacgc agcccgcgct ccggccttca gtcctcgcgg
    14641 cggcggaagg gcttgccggc acgctcgccc tggcgcttca ccggcgcgga gtggccgcca
    14701 gccttgcggt cggtggccgg cgcgcggcgt tcgtcgtgcg gtcgacgctc gtcgtggccg
    14761 cgaaagcccc cgccacccgg cttgccggcg taacccgcgc caggctggtt cgggcgcgcg
    14821 ccaccgaagt cgcgcgggcg gcgctcgccg tcctgcgcgt gcgagccgtc cgtgccgcgt
    14881 tccggccgcg ccgggcgccg gcgttcgggg accgtggccg cctcggcggc gctcagctcg
    14941 cggatcgaca gcggcatctg gcgcacgcgg gtgcgcttga gcgtggcgag caggtcctcg
    15001 ggcaggttgg cgggcaactc gacggtggag aactcgtcga agaggtggat ctggccgatg
    15061 aacctgccct cgatgccgcc ttcgttggcg atcgcgccga cgatctcctt ggggctcgcg
    15121 ccatgggcgc ggccgacctc gatgcggtag cgcaccagcg cgccttcgga gaaggaacgc
    15181 cggcgggcga ggatctcgtc gcggttgggg cgcggcgtgc gctcgcgcgc ggcgccggtg
    15241 gtgaagtgct cggcgcgcgg cgcgcggcct tcactgggcg tcgtggtggc gatgtcccag
    15301 ccgcggccgg actgctcgac ctggagcggg cgctcgcgct gggcgagcag ggccagtgcg
    15361 gcggcgacct cgtggatctc gagctcgttc tcgtcgatca ggtcgttgac cacgcccatg
    15421 aagaagccga gctccgcgcc ggcgatggtc tcggcgacct ggcgcttgaa ctgggccacg
    15481 cgcaggttgg tgacctcctc gccgctcggc agcgcgatcg gcgtgatcgg ctggcgggtg
    15541 gcgcgctcga tcatcttgag catgcgcgtc tcgcgcggcg ccacgaacag gatcgccacc
    15601 ccctcgcgcc cggcgcggcc ggtgcggccg atgcggtgca cgtaggcctc ggtgtcgtag
    15661 gg
//
LOCUS       DAOQBS010000068        15642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2858_length_15642_cov_15.895554, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000068 DAOQBS010000000
VERSION     DAOQBS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 15642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15642
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2858_length_15642_cov_15.895554"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /gene="ychF"
                     /locus_tag="PKN25_09125"
     CDS             complement(<1..626)
                     /gene="ychF"
                     /locus_tag="PKN25_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNV91103.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIQAENYPFCTIEPNVGIV
                     EVPDPRLAALSEIVKPQKIQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVH
                     VVRCFADDNVIHVSGSVDPIRDIEVIDTELALADMATVEKALNRYKRPAASGDKEAKI
                     LVAVLEKCFAQLDQGKAVRALDLSKEEWASLKPFCLITAKPVLYAANVA"
     gene            1048..2022
                     /locus_tag="PKN25_09130"
     CDS             1048..2022
                     /locus_tag="PKN25_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HNV91104.1"
                     /translation="MNINWIEAIDACSNAALASGALVPVQAEQVEMEDAGMRFIVRWV
                     SSLAAKDASRSAAPAQADAAGDRAGKAEGVQGGVVNGKVTKDDRTVAIPGGPRDPNFN
                     PFLNPDPALTVGRVGDAHVAILNKFPLCARHLVLARREFEEQLRPLARSDFAALAELM
                     GEAGGIGLYNGGTAAGASQRHKHVQWMPDDAGNASLRLFAPGLPAGLAEQGMATHPAL
                     PMRHCFVRVRCGRGEAVETAADSLQAGFERACAELGLGAGEDGLLPPFNLLAGDGWLL
                     AIPRSREHFEGISMSAVCFGGTLYTRHREQIEAIRAVGPLRALAAVGC"
     gene            complement(2029..2709)
                     /gene="pth"
                     /locus_tag="PKN25_09135"
     CDS             complement(2029..2709)
                     /gene="pth"
                     /locus_tag="PKN25_09135"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNV91105.1"
                     /translation="MSNAAARPPRLIVGLGNPGAEYSETRHNAGFWFCERLADQLGAR
                     YSFESRFHGLVANAREAGMWLLMPQTFMNRSGQAIGALARFYRIEPAEILVVHDELDI
                     PPGQLRLKFGGGLGGHNGLKDTSAHLGTNDYWRLRIGIGHPGDRNEVVNFVLKPARRE
                     EQQQIDEALDKALAVWPQIVRGELNAAATRLNVRPAPPKPPKPPKTPRPAQPDAGTEP
                     PATDGSAA"
     gene            complement(2743..3345)
                     /locus_tag="PKN25_09140"
     CDS             complement(2743..3345)
                     /locus_tag="PKN25_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNV91106.1"
                     /translation="MQIEFKATKRDAQGTGASRRLRRAGRIPGILYGGAGETQPVSMD
                     HNELYHLLRKEAFHASVLSIEVDGAKQNAVLRDVQWHPFKQQVLHLDFQRIAAGEKMH
                     LKVPLHFVGADVAPAVKLGGCMISHTINELDVQCLPTDLPEFIEVDLSALEAGQSVHV
                     SQIKLPAGVELVQHGEGDPVVATALQVKGAAEAEGEGEQA"
     gene            complement(3433..4383)
                     /locus_tag="PKN25_09145"
     CDS             complement(3433..4383)
                     /locus_tag="PKN25_09145"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNV91107.1"
                     /translation="MPHGSLMVFTGNANPKLGADVTRRLGISLGSATVGRFSDGEVNV
                     ELLENVRGKDVFVLQPTCSPTNDNLMELLVLVDALKRASAGRITAAIPYFGYARQDRR
                     PRSARVPITAKVVANMLQAVGVQRLLTMDLHADQIQGFFDIAVDNVYAAPVLLADLDK
                     QKYDDLLVVSPDVGGVVRARAFAKRMECDLAIIDKRRPKANVSEVMNIIGEVEGRTCV
                     IMDDIVDTAGTLCKAAAALKKHGAKRVLSYCTHAVLSGAAVSRINESELDELVVTDTI
                     PLRDDAKACPRIRQISVASLLADTILRISNEESVSSLFME"
     gene            complement(4475..4551)
                     /locus_tag="PKN25_09150"
     tRNA            complement(4475..4551)
                     /locus_tag="PKN25_09150"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4515..4517),aa:Gln,seq:ttg)
     gene            complement(4591..5478)
                     /gene="ispE"
                     /locus_tag="PKN25_09155"
     CDS             complement(4591..5478)
                     /gene="ispE"
                     /locus_tag="PKN25_09155"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNV91108.1"
                     /translation="MDRPALNTLHTPDCSDPARLAACPAPAKLNLFLHVVGRRADGYH
                     LLQTAFRLLDWGDTIDFAVRTDGAIHRRNEVPGVPEAADLVVRAARLLQAHTGCALGA
                     DITVHKVLPMGGGIGGGSSDAATTLIALNRLWGTGLSRAQLQALGLQLGADVPVFVYG
                     RDAFAEGVGEALQALELAPAWYVVISPGVQVPTAEIFSARDLTRNTPPIRMADFAAST
                     TRNDLQPVACSRYPEVLRVIEWLDVHAPARMTGSGACVFAEVSSEEEADRITKDCPPR
                     WKAWKARSIARHPLYEWLQ"
     gene            complement(5460..6062)
                     /gene="lolB"
                     /locus_tag="PKN25_09160"
     CDS             complement(5460..6062)
                     /gene="lolB"
                     /locus_tag="PKN25_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HNV91109.1"
                     /translation="MSRRPPHRSQGLRRTLLGGACALLLAACTPTLVRDGAQPASRPF
                     LPAFAVEGRLSATDGRNAASGRFEWVHDDEQDRLTLFSPLGQVVALLEGDPAGASLEA
                     ADGSRREAADVDALLPEALGVDLPAARLPRWIQAAPAADAEVRILDAAGRPQLVFDQG
                     WRIDYAGYADDTAAAAPQRLEISRGDARVRLIIDSWTALP"
     gene            complement(6059..7774)
                     /locus_tag="PKN25_09165"
     CDS             complement(6059..7774)
                     /locus_tag="PKN25_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV91110.1"
                     /translation="MKTQLARIARSLCLAFALGAGGVATAASGEAGEGNLPAQELTPR
                     TLYHFLLAEIAGARGQIGLSAQLYLDLARSTRDPRIARRATEIAMYSRNLVMARGAAE
                     IWTEVAPDSDEARRVLAGLSGAGRGEDINLEAIQFQLARVLAQSNGRLAQNLLSLGHT
                     LARVPDKQAVRGIVMRLTEPYVEMPEAHIARAQAAQAVEDRMGALAAVDRALELRSGW
                     EPAVLLKVQILQQAGAHTEALRVLEAEAARAPASRSLRLAKARALVSAQRFGEARAAF
                     NQLLEASPQDPELLYAVGLLSMQLEDFAAAELHFARALAAEHPQPDLIRLHLGQIAAD
                     RGEGERARKWFGEIESEDLRPEADIRSALSLAHEGRIEEARALLRNDVEDPDLARRYL
                     LAEAQILRDAERPTEALALLDAALRENPEDTGLLYEAAMLAERIGRMDLLEARLRRVL
                     ELQPDHAHALNALGYSLADRGLRLDEAEALIARAHALMPQDPFILDSLGWVRFRRGDQ
                     VGALVHLERAYGMRKDAEIAAHLGEVLWTLGRRDEARRIFAEALAAHPDNRLLTDTGR
                     RLGIQ"
     gene            7974..8792
                     /gene="mutM"
                     /locus_tag="PKN25_09170"
     CDS             7974..8792
                     /gene="mutM"
                     /locus_tag="PKN25_09170"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNV91111.1"
                     /translation="MPELPEVETTCRGIRPHVQGRSLSRLVVRNPRLRVAVPADLPAH
                     IEGARLEAVDRRAKYLLLRFPAGTVIVHLGMSGSLRVVAAAEPAGAHDHVDFVFGECA
                     LRLRDPRRFGMVVWQPGDVAAHPLLAGLGPEPLGDGFDADWLLRITRGLRAPIKHVLM
                     DSRKLVGVGNIYASESLFRARIHPLEPAGRLGPRRCARLVDCVRETLAAAIAAGGSTL
                     RDFVGGDGRPGYFQQQYFVYGRDGESCRVCGTPVRRIVSAQRASFFCPRCQRRS"
     gene            8956..10920
                     /locus_tag="PKN25_09175"
     CDS             8956..10920
                     /locus_tag="PKN25_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNV91112.1"
                     /translation="MTDLTEQFSAYSRWRGHASDAVARLRAWLARNDVGNAQADMRLQ
                     YLLDRLRDDKLTVAFVAEFSRGKSELINAIFFADHGDRILPSSAGRTTMCPTELQWQK
                     GAQPELRLLPIRSRARQTPVSELKRYPEEWTVIALDAVDAPGLQAALARVGETERVEV
                     AEAQALGFRVDAAGDDGIRPDAEGRIEIPRWRHAVIQFPHPLLEQGLVVLDTPGLNAI
                     GAEPELTLSMLPNAHAVLFILAADTGVTQSDLAVWRNYVQAGSERQRGRLVVLNKIDG
                     LWDGLRDEARIEAEIGRQVDSVAATLGVEPAAVFPVSAQKGLVARIQGDEALLARSRV
                     PQLERALAEGLLPAKQEIVREDILAESAEVAAATRSLLEARLAGLREQLQELTDLRGK
                     NQSVIEYMMRKIRSEKEEFEQGLQKYYAVRTVFTDLANNLFAHMGLDAVRDETRRTRE
                     AMLESSFTRGLRNAMQGYFRSLRGNLQRSTEEIGEITRMLDAMYKRFSVEHGLKLTAP
                     EGFSTLRYEKELERLETAFNRQINTALTLVTTEKHALTQKFFETVAVQARQTFELANR
                     DVEQWLRAVMSPLETQVREYQIQLKRRLESVKRIHEATDTLESRVEELMQGEEALRAL
                     LDELDNLEAAVADALDVPANTPVTGVRAAA"
     gene            complement(11011..11262)
                     /locus_tag="PKN25_09180"
     CDS             complement(11011..11262)
                     /locus_tag="PKN25_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017034990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNV91113.1"
                     /translation="MALMITDECINCDVCEPECPNGAIYQGEEIYEIDPNKCTECVGH
                     FDEPQCQQVCPVDCIPLDPKREETKDQLMAKFLKLTGKA"
     gene            complement(11282..11770)
                     /gene="coaD"
                     /locus_tag="PKN25_09185"
     CDS             complement(11282..11770)
                     /gene="coaD"
                     /locus_tag="PKN25_09185"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNV91114.1"
                     /translation="MKEAIAVYPGTFDPFTRGHEDLVRRASILFEKVVVAVARSNSKN
                     PIFGLEDRVDIARDAVSAFPNVEVVGFDCLLMEFLQQQDARVIIRGLRAVSDFEYEFQ
                     MAGMNRKLYPDVETVFLTPGEEFMFISATMVREIARLGGDVSKFVQPRVLARLQERLK
                     SK"
     gene            complement(11767..12306)
                     /gene="rsmD"
                     /locus_tag="PKN25_09190"
     CDS             complement(11767..12306)
                     /gene="rsmD"
                     /locus_tag="PKN25_09190"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNV91115.1"
                     /translation="MSRVRIVGGQWRSRLLDVAAVPGLRPTPDRVRETLFNWLGQDLD
                     GHACLDLFAGTGILGFEAASRGAARVVLVEHDARALDALHKAAKTLQAAQVEIVRGDA
                     VRFAQSTPQRFDGVFLDPPYKQGWIERVSPWLDRLVRPEGWLYVESEVAVDSLGAWRA
                     VKQGRAGQVHFHLMRRSEE"
     gene            complement(12303..13706)
                     /locus_tag="PKN25_09195"
     CDS             complement(12303..13706)
                     /locus_tag="PKN25_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV91116.1"
                     /translation="MTAETPSLERSPTRPRRLHLLRTAAFVLGAGAAAGALAAPEIVE
                     WTAPTGARVHFVESRALPLVDIQISFAAGGALDPEGKAGLASMTQALLDAGAGGLDEQ
                     TIADRKADLGIELSGGTDNDRASMALRSLSSPAELDAAVELAATLLARPDFPAAVLER
                     ERSRAIAGLREALTKPATLAARQFNAALYAGHPYGHDSTPESLAAIGRDDLVDFHRRH
                     YGANRATVAIVGDVDRAAAERIAVRLTAGLPATQAAMPLPAPAPTTARTLRIPHPSAQ
                     AHILVGQPGMAREDADYFPLLVGNYVLGGGGFVSRLTAEVREKRGFAYSVYSALSPQQ
                     VAGPFQIGLQTRGSQAEEALAVVRATLAGFLADGPSAEELQAAKDNLINGFGLRLDSN
                     AKLLDYVSMIGFYRLPLDWLERYPQQVAAVDVAAVRAAWQRRIRPEQLVTVIAGGDGD
                     RPAAAGASTTPPAEAAQ"
     gene            complement(13733..15145)
                     /locus_tag="PKN25_09200"
     CDS             complement(13733..15145)
                     /locus_tag="PKN25_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV91117.1"
                     /translation="MFRLPPALRLARLPGSRLARGLLLGATLSAALAPPVLANPFETT
                     LANGMKLIVKEDRRAPSVVHMVWYRTGAMDEPDGVSGVAHVLEHMMFKGTREVGPGEF
                     NRRVAAVGGRDNAFTGKDYTAYFQQVPPAHLPAMMALEADRMKNLVLTDEEFAREIEV
                     VKEERRLRTDDQPRALVFEQLMATAYQAHPYRRPVIGWMPDLEAMRPEDARTWYRRWY
                     APNNAYLVVVGDVDHREVFRHAEQTYGALPAGELPARRISPEPAQRGPRASTVKAPAE
                     LPYLAMAWHVPALRDPANDREAFALDVLAAVLDGYDGARLTRGLVRDQRIAVSVGASY
                     DTGNRGPALFYLHGVPAAGTSPEALAEALRAELRRIADEGISAAELARVKTQAIAAQV
                     YKRDSLMGQAMEIGHLESAGLSWRDEDRLLDGLRAVTAEEVQAVAKRYFDDASLNTAR
                     LDPQPLGDKRPRSSFAAPRH"
     gene            15310..>15642
                     /locus_tag="PKN25_09205"
     CDS             15310..>15642
                     /locus_tag="PKN25_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNV91118.1"
                     /translation="MFGFLKKKFGKSVDEARPAEQAAEEQTASEVRAEDLAGAPAPQA
                     PAEASVAEAARRDAAEIDATAEPVEPAALPESPAQPAAEAAALAEPEPAAQAPAEAVP
                     EPQPEPPGE"
BASE COUNT         2214 a         5181 c         5855 g         2392 t
ORIGIN      
        1 gcgacgttgg cggcgtagag caccggcttg gcggtgatca ggcagaaggg cttgaggctg
       61 gcccattctt ccttcgacag gtcgagcgcg cgcacggcct tgccctggtc gagctgggcg
      121 aagcacttct cgagcacggc gacgaggatc ttggcctcct tgtcacccga ggcggcaggg
      181 cgcttgtagc ggttgagcgc cttctccacg gtggccatgt cggcgagggc gagctcggtg
      241 tcgatgacct cgatgtcgcg gatcgggtcg acactgccgg agacgtggat cacgttgtcg
      301 tccgcgaagc agcgcacgac gtgcacgatg gcgtcggtct cgcggatgtt ggcgaggaac
      361 tggttgccca ggccttcgcc cttggaggcg ccggcaacca ggccggcgat gtcgacgaac
      421 tcgacgatgg cgggctggat cttctgcggc ttgacgatct cggacagcgc ggccaggcgc
      481 gggtccggca cctcgacgat gccgacgttg ggctcgatgg tgcagaaggg gtagttctcg
      541 gcctggatgc cggccttggt cagcgcgttg aagagggtcg acttgccgac gttgggcagg
      601 ccgacgattc cgcatttcag gctcatgtga atccttgatc gttccggggg ctgcgtcgcc
      661 gcatcggggg ctacggcctt gatccggtat ggaaagccgg agattgtacc gtgtcgcggg
      721 gtagggctcc aaagcgggcg cggggtcggt ccgtgcgcgg actgcggggt agggcggcgg
      781 tggggtgccg cggttgggtg cggcttcgat ccgaccgcgg ggccggggcg gggcacgatc
      841 tcttggtgca tttccgcagc cgtgccggtt tccgtaggag cgacgcgagt cgcgatcgcc
      901 acctccgcat ccaccgtggc ctggccgacc gcgtcgtcgc ggctcctgcg gccatcctcg
      961 ccatttgcgg ccaggagcca ttcggttcgc tgccggccta caatcgccca tcctgcattt
     1021 gcccaaaaac acaccggagg agaagggatg aacatcaact ggatcgaggc gatcgatgcg
     1081 tgcagcaacg cggcgctggc gtcgggggcg ctggtgccgg tgcaggccga gcaggtcgaa
     1141 atggaagacg cgggcatgcg cttcatcgtg cgctgggtgt cctcgctcgc ggccaaggat
     1201 gcgagcagga gcgccgcgcc ggcgcaggcc gacgccgctg gcgacagggc gggcaaggcc
     1261 gaaggcgtgc aaggtggggt ggtcaatggc aaggtgacca aggacgacag gacggtggcg
     1321 atccccggcg ggccgcgcga tccgaacttc aatcctttcc tgaatcccga cccggcgctc
     1381 accgtcggac gggtgggtga tgcccatgtg gcgatcctga acaagttccc cctgtgcgcg
     1441 cgccacctgg tgctggcgcg gcgggagttc gaggagcagc tgcggccgct cgcgcgcagc
     1501 gactttgccg cgctcgccga gctgatgggc gaggcgggcg ggatcgggct ctacaacggc
     1561 ggcaccgctg cgggggcgag ccagcgccac aagcacgtgc agtggatgcc cgacgatgcc
     1621 ggcaacgcca gcctgcggct gttcgcgccc gggctgcccg cagggctggc ggagcagggg
     1681 atggcgacgc atccggcgct gccgatgcgg cattgcttcg tgcgcgtgcg ctgcggtcgc
     1741 ggcgaggcgg tggagacggc ggccgacagc ctgcaggccg gcttcgagcg tgcgtgtgcc
     1801 gagctcggcc tgggagcggg ggaggacggg ctgctgccac ccttcaacct gctcgcgggc
     1861 gatggctggc tgctggcgat tccgcgcagc cgggagcact tcgaggggat ctcgatgagc
     1921 gcggtgtgct tcggtggcac gctgtatacg cgccatcgcg agcagatcga ggcgatccgc
     1981 gcggtcgggc cgctgcgcgc gctggcggcg gtgggctgct gagcgggatc aggcggcgga
     2041 gccgtccgtc gcgggcggtt ccgtgccggc gtcgggttgc gcgggcctgg gggtcttggg
     2101 aggcttcggg ggcttgggcg gcgcgggacg tacgttgagc cgggtcgccg cggcattgag
     2161 ttcgccgcgc acgatctgcg gccagaccgc gagcgccttg tcgagcgctt cgtcgatctg
     2221 ctgctgttct tcgcgccgcg cgggcttgag cacgaagttc accacctcgt tgcggtcgcc
     2281 cggatggccg atgccgatgc gcaggcgcca gtagtcgttg gtgccgaggt gggcggaggt
     2341 gtccttgagg ccgttgtggc cgccgaggcc gccgccgaac ttgaggcgca gctggccggg
     2401 ggggatgtcg agctcgtcgt gcacgacgag gatctcggct ggttcgatgc ggtagaagcg
     2461 cgcgagcgca ccgatcgcct ggccggagcg gttcatgaag gtctgcggca tcagcagcca
     2521 catgcccgct tcacgcgcgt tggccaccag gccatggaaa cgcgactcga acgagtagcg
     2581 cgcgccgagc tggtcggcga gccgctcgca aaaccaaaac ccggcgttgt gccgggtttc
     2641 ggagtattcg gcgcccggat tgccgaggcc gacgatcaga cggggcggac gcgcggcggc
     2701 gttgctcatc ggtcgtcggt ctcctgcatc cgggccgggc gatcaggcct gctcgccttc
     2761 gccttcggct tcggcggcac ccttcacctg cagcgcggtc gcgaccaccg ggtcgccttc
     2821 gccgtgctgc accagctcga cgccggccgg cagcttgatc tgcgagacgt gcaccgactg
     2881 gccggcttcc agcgccgaca ggtcgacctc gatgaactcg ggcaggtccg tgggcaggca
     2941 ctgcacgtcg agttcgttga tggtgtgcga gatcatgcag ccgccgagct tgacggcggg
     3001 ggcgacgtcg gcgccgacga agtgcagcgg caccttcagg tgcatcttct cgcccgcggc
     3061 gatgcgctgg aagtcgaggt gcagcacctg ttgcttgaac gggtgccatt gcacgtcgcg
     3121 cagcacggcg ttctgcttgg cgccatcgac ctcgatcgag agcacggaag cgtggaaggc
     3181 ttccttgcgc agcaggtggt agagctcgtt gtggtccatc gacaccggct gggtttcgcc
     3241 cgcaccgccg tagaggatgc ccgggatccg gccggcgcga cgcaggcggc ggctcgcacc
     3301 cgtaccctgc gcgtcacgct tggtggcctt gaattcgatt tgcatgtgat gctcctgagt
     3361 gggttgtccc cgcccgcgac caggcggaga cggtggatgc ccgtccgacg cggggtcgga
     3421 agggcgaaag ccttattcca tgaacagcga ggagaccgac tcctcgttgc tgatgcgcag
     3481 gatggtgtcg gcgagcagcg aggccaccga gatctggcgg atgcgcgggc acgccttggc
     3541 gtcgtcgcgc agcgggatgg tgtcggtgac gaccagctcg tcgagctcgg actcgttgat
     3601 gcgcgagacc gccgcgcccg acagcaccgc gtgggtgcag taggacagca cacgcttggc
     3661 gccgtgcttc ttgagcgcgg cggcagcctt gcacagggtg ccggcggtgt cgacgatgtc
     3721 gtccatgatc acgcaggtgc ggccctcgac ctcgccgatg atgttcatca cctcggagac
     3781 gttggccttg gggcggcgct tgtcgatgat cgccaggtcg cactccatgc gcttggcgaa
     3841 ggcgcgcgcg cgcaccacgc cgccgacgtc gggcgagacg acgagcaggt cgtcgtactt
     3901 ctgcttgtcg aggtcggcga gcagcaccgg cgcggcgtac acgttgtcga ccgcgatgtc
     3961 gaagaagccc tggatctggt cggcgtgcag gtccatggtc agcaggcgct ggacgcccac
     4021 cgcctgcagc atgttggcga ccaccttggc ggtgatcggc acgcgcgccg agcgcgggcg
     4081 gcgatcctgg cgggcgtagc cgaaataggg gatggcggcg gtgatgcggc cggcggaggc
     4141 gcgcttgagg gcgtcgacca gcaccagcag ttccatcaga ttgtcgttgg tcggggagca
     4201 ggtgggctgc aggacgaaga cgtccttgcc gcgcacgttc tcgagcagtt cgacattgac
     4261 ctcgccgtcc gagaagcggc cgacggtggc cgagccgagg gagatcccca ggcgtcgcgt
     4321 cacgtcggcg ccaagcttgg ggttggcgtt gccggtgaag accatcaggc tgccgtgggg
     4381 catttctgct actccgagtg ccgtaaggaa cgacgagcga atattcggcg cgtaaaaacg
     4441 actcgggcag acccatggtc tgcccgtcgt tgtatggctg gggaggaagg attcgaaccc
     4501 tcgaatgccg gaatcaaaat ccggtgcctt aaccgacttg gcgactcccc aagaaacctt
     4561 tttcgctgcg cgtcgagtgc tgcgcgggcg ctattgtaac cactcgtaaa gcggatggcg
     4621 agcgatgctg cgcgctttcc aagccttcca gcgtggcgga caatcttttg tgatgcggtc
     4681 ggcctcttct tccgaggaga cttccgcgaa cacgcaggcg cccgagcctg tcatgcgggc
     4741 tggtgcatgc acatccagcc actcgatcac acgcaagact tcggggtagc gactgcacgc
     4801 aaccggctgc aggtcgtttc gcgtagtgct tgctgcgaag tccgccattc tgatgggggg
     4861 cgtatttctc gtcaagtccc tcgccgaaaa aatttcggcg gtcggcacct ggaccccggg
     4921 cgagatcacc acgtaccagg ccggcgccag ttcgagcgcc tgcagcgcct cgcccacgcc
     4981 ttcggcgaag gcgtcgcggc cgtagacgaa caccggcacg tcggcgccga gctgcagccc
     5041 gagcgcctgg agttgcgcgc gtgacagccc ggtgccccaa aggcggttga gcgcgatcag
     5101 cgtggtcgcc gcgtccgaac tgccgccccc gatcccgccg cccatcggca gcaccttgtg
     5161 caccgtgatg tcggcgccga gcgcgcagcc ggtgtgcgcc tgcagcaggc gggcggcgcg
     5221 caccaccagg tccgcggcct cgggtacgcc gggcacctcg ttgcggcggt ggatcgcgcc
     5281 gtccgtgcgt acggcgaagt cgatcgtgtc gccccagtcg agcaggcgga atgcggtctg
     5341 cagcaggtgg tagccgtcgg cgcggcggcc gacgacgtgg aggaagaggt tgagcttggc
     5401 cggcgcgggg caggcggcca gtcgcgcggg atcgctgcag tcgggggtgt gcagggtgtt
     5461 cagggcaggg cggtccatga atcgatgatc agacgcacgc gggcatcgcc gcgcgagatt
     5521 tcgaggcgtt gcggtgcggc cgcggcggtg tcgtcggcat agccggcgta gtcgatgcgc
     5581 cagccctggt cgaagacgag ctgcggacga ccggcggcgt cgaggatgcg cacctcggcg
     5641 tcggccgccg gggcggcctg gatccagcgc ggcaggcgcg cggcgggcag gtcgacgccg
     5701 agcgcctcgg gcagcagcgc gtccacgtcg gcggcctcgc gccggctgcc gtcggcagcc
     5761 tccaggctgg cgcccgcagg atcgccctcg agcagggcga cgacctggcc gagggggctg
     5821 aacaaggtga ggcggtcctg ctcgtcgtca tgcacccatt cgaagcgccc gctcgccgcg
     5881 ttacggccgt cggtggccga cagccgcccc tcgaccgcga acgcgggcag gaagggacgc
     5941 gaggcgggct gagcgccgtc gcgcaccagc gtcggcgtgc atgccgcaag cagcagtgcg
     6001 caggcgccgc ccagcagggt ccggcgcagg ccttgcgagc gatgcggggg gcgtcggctc
     6061 actggatgcc cagtcggcgg ccggtgtccg tcagcagacg gttgtcgggg tgggctgcga
     6121 gcgcctcggc gaagatccgt cgggcctcgt cccgacggcc gagtgtccat agcacctcgc
     6181 cgaggtgggc ggcgatctcg gcgtccttgc gcatgccata ggcgcgctcc aggtggacga
     6241 gcgcgccgac ctggtcgccg cggcggaagc gcacccagcc gaggctgtcg agaatgaagg
     6301 ggtcttgcgg catgagcgca tgcgcgcgtg cgatcagcgc ctcggcttcg tccaggcgca
     6361 ggccgcggtc ggcgagcgaa taaccgagcg cgttgagcgc atgcgcatga tcgggctgca
     6421 gctcgagcac gcggcgcagg cgcgcttcga gcaggtccat gcgaccgatg cgctcggcga
     6481 gcatggcggc ctcgtagagc aggccggtgt cttcggggtt ctcgcgcagg gcggcatcga
     6541 gcagcgcgag ggcctcggtc ggacgctcgg cgtcgcgcag gatctgcgcc tcggcgagca
     6601 ggtagcggcg ggcgaggtcg ggatcctcga cgtcgttgcg taacagggcg cgcgcctctt
     6661 cgatgcgtcc ttcgtgcgcg aggctcaggg cgctgcggat gtccgcctcg ggacggaggt
     6721 cctcgctctc gatctcgccg aaccacttgc gtgcgcgctc gccctcgccg cggtcggcgg
     6781 cgatctggcc caggtgaagg cggatgaggt cgggttgcgg gtgctccgcg gcgagcgcgc
     6841 gtgcgaagtg cagctcggcc gcggcgaagt cctcgagctg catcgacagc aggcccaccg
     6901 catagagcag ttcgggatcc tgcggcgagg cttcgagcaa ctggttgaag gccgcgcgtg
     6961 cctcgccgaa gcgctgcgcg ctcaccagcg cacgcgcctt cgccaggcgc agcgaccggc
     7021 tcgccggcgc gcgcgcggcc tcggcctcga gcacgcgcag ggcctccgtg tgcgcgcctg
     7081 cctgctggag gatctgcacc ttcagcagca ccgcgggttc ccagccgctg cgcagctcga
     7141 gggcgcgatc caccgcagcg agcgcgccca tcctgtcctc gaccgcctgt gccgcctgcg
     7201 cgcgggcgat gtgcgcctcg ggcatctcga cgtagggttc ggtcaggcgc atcacgatgc
     7261 cgcgcaccgc ctgtttgtcg ggtacgcggg cgagggtatg gccgaggctc agcaggttct
     7321 gcgcgaggcg gccgttcgat tgcgcgagca cgcgcgcgag ctggaactgg atggcctcga
     7381 ggttgatgtc ctcgccgcgg ccggcgccgc tcagcccggc gagtacccgg cgcgcctcgt
     7441 ccgagtcggg ggcgacctcg gtccagatct ccgcggcgcc gcgcgccatc acgaggttgc
     7501 gcgagtacat cgcgatctcg gtggcgcggc gggcgatgcg cgggtcgcgc gtgctgcgcg
     7561 cgaggtcgag atagagctgt gccgacaggc cgatctggcc gcgcgcgccg gcgatctcgg
     7621 cgagcaggaa gtggtacagc gtgcgcggcg tgagttcctg cgcgggcagg ttgccctcgc
     7681 cggcctcgcc gctggccgcc gtcgcgaccc cgccggcgcc gagcgcgaag gccaggcaga
     7741 gggagcgggc gatgcgggcg agctgggttt tcatgggagg aattccggga ccttcgatgg
     7801 gttgcgccag agccgcggcg caagaaacgc ctggcacccg ccggggggtg ccgcgaaagc
     7861 ttcgagtgct cggctacaat tcggttcatg ccatggtagg cagatcgccg cccctgccgc
     7921 aacccgttcc ccgtgtgcgc ccgcgcgcgg gcagcccacg gattcctttc ccgatgcccg
     7981 aactgcccga agtcgagacc acctgccgtg gaatccggcc gcacgtccag ggccgcagcc
     8041 tgagccggct ggtggtgcgc aatccgcgcc tgcgcgtggc ggtgcccgcc gacctgcccg
     8101 cgcacatcga gggcgccagg ctcgaggcgg tcgaccggcg tgccaagtac ctgctgctgc
     8161 gttttcccgc gggcacggtg atcgtgcatc tcgggatgtc gggcagcctg cgcgtggtcg
     8221 cggcggccga gccggcgggc gcgcacgacc acgtcgattt tgtcttcggc gagtgcgcgc
     8281 tgcgtctgcg cgacccacgc cgcttcggca tggtggtgtg gcagcccggc gacgtggcgg
     8341 cgcatccgct gctcgccggc ctggggccgg aaccgctcgg cgacggcttc gacgccgact
     8401 ggctgctgcg catcacgcgc ggtctgcgcg cgccgatcaa gcatgtgctg atggacagcc
     8461 gcaagctcgt cggcgtgggc aacatctacg cctccgagag cctcttccgc gcccgcatcc
     8521 atccgctcga gcccgccggc cggctcgggc cgcgacgctg cgcgcgcctg gtcgattgtg
     8581 tgcgcgagac gctcgccgcc gcgatcgcgg ccggcggcag cacgctgcgc gactttgtcg
     8641 gcggcgacgg ccggccaggc tatttccagc agcagtactt cgtctatggt cgcgacggcg
     8701 agtcctgccg ggtgtgcggc acgccggtgc ggcgcatcgt cagcgcccaa cgcgcgagct
     8761 tcttctgtcc gcgctgccag cgccgcagct gaaaggacgg cgatgccccg gtggggggca
     8821 tgcaggcggc gtgcaatttg gcacgctcgc gaaccgcgaa gcgggttatg gtgaataatg
     8881 ttctgcgctg cgggccggcg aggccttgcc ctcgcgcgcg cccgacgccc ccccgcctgc
     8941 cggacgagca cgccgatgac cgatctcacc gaacagtttt ccgcctacag ccgctggcgc
     9001 ggccacgcca gcgacgcggt ggcccgcctg cgcgcgtggc tggcgcgcaa cgacgtgggc
     9061 aacgcccagg cggacatgcg cctgcaatac ctgctcgacc gcctgcgcga cgacaagctc
     9121 acggtggcct tcgtcgccga gttctcgcgt ggcaaatccg agctcatcaa cgcgattttc
     9181 ttcgccgacc acggcgaccg catcctgccc tccagcgcgg gccgcaccac gatgtgcccg
     9241 accgagctgc agtggcagaa gggggcgcag cccgagctgc gtctgctgcc gatccgcagc
     9301 cgcgcgcgcc agacgccggt gagcgagctc aagcgctatc ccgaagaatg gaccgtgatc
     9361 gcgctcgacg cggtcgacgc ccccggcctg caggccgcgc tcgcgcgcgt gggcgagacc
     9421 gagcgggtcg aggtcgccga agcgcaggcg ctcggcttcc gcgtcgatgc cgcaggggac
     9481 gacggcatcc gtcccgatgc cgagggtcgc atcgagatcc cgcgctggcg ccatgccgtg
     9541 atccagttcc cgcatcccct gctcgagcag ggcctggtgg tgctcgacac gccgggcctc
     9601 aatgcgatcg gcgccgagcc cgagctcacc ctgtcgatgc tgccgaatgc gcacgcggtg
     9661 ctgttcatcc tcgccgccga caccggcgtc acccagagcg atctcgcggt gtggcgcaac
     9721 tacgtgcagg ctggcagcga gcgccagcgc gggcgcctgg tcgtgctcaa caagatcgac
     9781 gggctgtggg acgggctgcg cgacgaagcg cgcatcgaag ccgagatcgg ccgccaggtc
     9841 gactcggtcg ccgcgaccct cggcgtcgag cccgccgcag tgttcccggt gtcggcgcag
     9901 aagggcctgg tggcgcgcat ccagggcgac gaggccctgc tcgcgcgcag ccgtgtgcca
     9961 cagctcgagc gtgcgctcgc cgaggggctg ctgcccgcca agcaggagat cgtgcgcgag
    10021 gacatcctcg ccgagtccgc cgaggtcgcc gccgccacgc gcagcctgct cgaggcgcgc
    10081 ctggccggct tgcgcgaaca gctccaggaa cttaccgacc tgcgcggcaa gaaccagagc
    10141 gtcatcgaat acatgatgcg caagatccgc agcgagaagg aggagttcga gcagggtctg
    10201 cagaagtact acgccgtgcg tacggtgttc accgacctcg ccaacaacct gttcgcgcac
    10261 atgggcctgg acgcggtgcg cgacgagacg cggcgcacgc gcgaggcgat gctcgaatcc
    10321 agcttcacgc gcgggctgcg caacgccatg cagggctatt tccgcagcct gcgtggcaac
    10381 ctgcagcgct cgaccgagga gatcggcgag atcacccgca tgctcgacgc catgtacaag
    10441 cgcttcagcg tcgagcacgg cctcaagctc accgcacccg aaggcttctc gacgctgcgc
    10501 tacgagaagg agctcgagcg cctggagacg gccttcaacc gccagatcaa caccgcgctg
    10561 accctggtca ccaccgagaa gcacgcgctc acgcagaagt tcttcgagac cgtcgcggtg
    10621 caggcgcgcc agaccttcga gctcgccaac cgcgacgtcg agcagtggct gcgcgcggtg
    10681 atgtcgccgc tggagaccca ggtgcgcgag taccagatcc agctcaagcg ccggctcgag
    10741 agcgtcaagc gcatccacga ggccaccgac acgctggaga gccgcgtgga ggagttgatg
    10801 cagggcgagg aggcgctgcg cgcgctgctg gacgagctcg acaacctcga ggccgcggtc
    10861 gccgacgcgc tcgacgtgcc cgccaacacg cctgtgacgg gcgtgcgtgc ggccgcctga
    10921 gcggcgcgcg catgcaataa agcggacggg ggcgcgccga tggccgcccc cgtcgtctcg
    10981 tgcggcggga tgggctcccg ccgggtgtga tcaggccttg ccggtcagct tgaggaactt
    11041 ggccatcagc tggtccttgg tctcctcgcg cttggggtcg agcgggatac agtccaccgg
    11101 gcacacctgc tggcactgcg gctcgtcgaa gtggccgacg cactcggtgc acttgttggg
    11161 gtcgatctca tagatctctt cgccctggta gatggcgccg ttggggcact ccggttcgca
    11221 gacgtcgcag ttgatgcatt cgtcggtaat catcagagcc atggtcttcg cttccttgcg
    11281 tttactttga tttgagtctt tcctgcagcc gtgcgagcac cctgggctgc acgaacttgc
    11341 tcacgtcgcc gccgaggcgg gcgatctccc gcaccatcgt ggcggagatg aacatgaatt
    11401 cttcaccggg cgtcaggaac accgtctcga cgtccggata aagcttgcgg ttcatccctg
    11461 ccatctggaa ttcgtattcg aagtcggata ccgcacgcag cccgcggatg atcacgcgcg
    11521 catcctgctg ctggaggaac tccatcagca ggcagtcgaa gccgaccact tcgacgttgg
    11581 gaaaggctga gacggcatcg cgtgcgatgt cgacccggtc ctccagtccg aagatcgggt
    11641 tcttgctgtt gctgcgcgcg accgcgacga cgaccttctc gaacaggatc gaggcgcgcc
    11701 gcacgagatc ctcgtggccg cgggtgaacg ggtcgaaggt gcccggatag accgcgatcg
    11761 cttccttcat tcctcgctcc tgcgcatgag atggaaatgg acctgcccgg cgcggccctg
    11821 cttgaccgcg cgccatgcgc cgaggctgtc cacggcgact tcggattcga catagagcca
    11881 tccctcggga cggaccagcc ggtccagcca aggcgacacc cgctcgatcc agccctgctt
    11941 gtagggcgga tcgaggaaca cgccgtcgaa acgctgcggc gtggattgcg cgaatcttac
    12001 cgcatcgccg cgcacgatct ctacttgcgc cgcctgtagg gtctttgccg ccttgtgcag
    12061 tgcgtcgagc gcgcgcgcgt cgtgctcgac caggacgacg cgcgcggcgc cgcgtgaggc
    12121 ggcctcgaag ccgaggatgc cggtgccggc gaagaggtcc aggcaggcgt ggccgtcgag
    12181 gtcctggccc agccagttga acagggtctc gcgcacgcgg tcgggcgtcg ggcgcaggcc
    12241 tggcaccgcg gccacgtcga gcaggcgcga gcgccactgt ccgccgacga tgcggacccg
    12301 gctcactgcg cggcttccgc ggggggcgtg gtggacgcac ccgccgcggc gggacggtcg
    12361 ccgtcgccgc cggcgatcac ggtgacgagc tgttccgggc ggatgcggcg ctgccaggcc
    12421 gcgcgcaccg cggcgacgtc gaccgcggcg acctgctgcg ggtagcgctc cagccagtcg
    12481 agcggcaggc ggtagaagcc gatcatcgac acgtagtcga gcagcttggc gttggagtcg
    12541 aggcgcaggc cgaagccatt gatcaggttg tccttggcgg cctgcaactc ctcggcgctg
    12601 gggccgtcgg cgaggaagcc cgcgagcgtc gcccgcacca ccgcgagcgc ttcctcggcc
    12661 tggcttccgc gcgtctgcag gccgatctgg aaggggccgg cgacctgctg tggcgacagc
    12721 gcgctgtaca cgctgtaggc aaaaccgcgc ttctcgcgta cctcggcggt cagccgcgac
    12781 acgaagccgc caccgccgag cacgtagttg cccaccagca aggggaagta gtcggcgtcc
    12841 tcgcgcgcca tgccgggctg gccgacgagg atgtgggcct gcgccgaggg gtgcgggatg
    12901 cgcaaggtcc gggccgtggt gggcgcgggc gccggcagcg gcatggcggc ctgcgtcgcg
    12961 ggcaggcccg cggtgaggcg gaccgcgatg cgctcggcgg cggcgcggtc cacgtcgccg
    13021 acgatcgcta ccgtggcgcg gttggcgccg tagtggcggc gatggaagtc gaccaggtcg
    13081 tcgcgcccga tcgcggcgag cgactccggc gtgctgtcgt ggccataagg gtggccggcg
    13141 tagagcgcgg cgttgaactg gcgcgcggcg agcgtggcgg gcttggtcag cgcctcgcgc
    13201 aggccggcga tcgcgcggct gcgctcgcgc tcgagcaccg cggcgggaaa gtcggggcgg
    13261 gcgagcaggg tggcggcgag ctcgactgcg gcgtcgagct cggcgggcga ggacaggctg
    13321 cgcagcgcca tgctggcgcg gtcgttgtcg gtgccgccgg agagctcgat gccgagatcg
    13381 gccttgcggt cggcgatggt ctgctcgtcg agcccgccgg cgccggcgtc gagcagggcc
    13441 tgggtcatgc tcgccagccc ggccttgccc tcggggtcga gcgcgccgcc ggcagcgaag
    13501 gagatctgga tgtcgaccag cggcagcgcg cggctctcga cgaagtgcac ccgcgcgccg
    13561 gtgggcgcgg tccattcgac gatctcgggg gcggcgagcg cgccggccgc cgcgccggca
    13621 ccaagtacga acgcggcggt gcgcaggaga tggaggcggc gggggcgggt gggggagcgc
    13681 tcgaggctgg gggtttctgc ggtcatggcg ggcatttcgt gggcgttgcg cttcagtggc
    13741 gcggggcggc gaaggacgag cgcgggcgct tgtcgccgag cggctgcgga tcgaggcggg
    13801 cggtgttgag gctggcgtcg tcgaagtaac gcttcgctac cgcctgcacc tcctcggcgg
    13861 tgaccgcgcg caggccatcg agcagcctgt cctcgtcgcg ccaggacagg cccgcggatt
    13921 cgaggtggcc gatctccatc gcctggccca tcagcgagtc gcgcttgtac acctgggcgg
    13981 cgatcgcctg cgtcttcacg cgcgcgagct ccgcggcgct gatgccttcg tcggcgatac
    14041 ggcgcagctc ggcgcgcaag gcctcggcga gcgcctccgg gctggtgccg gcggcgggca
    14101 cgccgtgcag gtagaacagc gccgggccgc ggttgccggt gtcgtagctg gcaccgacgc
    14161 tgaccgcgat gcgctggtcg cgcaccaggc cgcgggtgag gcgggcgccg tcgtagccgt
    14221 cgagcacggc ggcgagcacg tcgagcgcga aggcctcgcg gtcgttggcg ggatcgcgca
    14281 gcgcgggcac gtgccaggcc atggcgagat agggcagctc ggcgggggcc ttcaccgtcg
    14341 aggcgcgcgg gccgcgttgc gccggttcgg gggagatgcg gcgggcgggc agctcgccgg
    14401 cggggagggc gccgtaggtc tgctcggcgt ggcggaacac ctcgcggtgg tcgacgtcgc
    14461 ccacgaccac caggtacgcg ttgttgggcg cgtaccagcg ccggtaccag gtgcgcgcgt
    14521 cttccggccg catggcctcg aggtcgggca tccagccgat caccgggcgg cggtaggggt
    14581 gggcctggta ggcggtggcc atcagctgct cgaacacgag cgcgcgcggc tggtcgtcgg
    14641 tgcgcaggcg gcgctcctcc ttgaccacct cgatctcgcg cgcgaactcc tcgtcggtga
    14701 gcacgaggtt tttcatgcgg tcggcctcga gcgccatcat cgccggcagg tgggcgggcg
    14761 gcacctgctg gaagtaggcc gtgtagtcct tgccggtgaa ggcgttgtcg cgcccgccga
    14821 cggcggccac gcggcggttg aactcgcccg ggccgacctc cctcgtgccc ttgaacatca
    14881 tgtgctcgag cacatgggcc acgcccgaga cgccgtccgg ctcgtccatc gcacccgtgc
    14941 ggtaccacac catgtgcacc accgaggggg cgcggcggtc ctccttgacg atcagcttca
    15001 tgccgttggc gagcgtggtc tcgaaggggt tggcgaggac gggcggggcg agcgcggcgg
    15061 agagcgtcgc gccgaggagg agcccgcgtg cgaggcggct tccgggcagg cgggcgaggc
    15121 ggagggcggg ggggaggcga aacatggagt tgtcctgcat ctgttagaat ccgcggcact
    15181 ccgcgaggcc cgctgcgcat ggcgcagcca gtgccgggga gccgcatctt agcgtgatcg
    15241 gcggtgcgcg cccatgcttg ccgggccgcg tgcgccgacg caaaacgtcc cctctgaccc
    15301 gagcgtttca tgtttggttt cctgaagaag aagttcggca agtctgtcga cgaggccagg
    15361 cccgccgagc aggctgccga ggagcagacc gcctccgagg tccgcgccga agacctcgcc
    15421 ggcgcgccgg cgccgcaagc gcccgcggag gcctcggtgg ccgaggctgc gcgtcgcgac
    15481 gcggcggaga tcgacgcgac cgcagagccc gtcgagcccg ctgcgctgcc cgaatcccct
    15541 gcgcagccgg ccgccgaggc ggcggccctt gccgagccgg agcccgctgc gcaggcgcca
    15601 gccgaagccg tgcccgagcc gcagccggaa cccccgggcg ag
//
LOCUS       DAOQBS010000069        15582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_2875_length_15582_cov_13.712243, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000069 DAOQBS010000000
VERSION     DAOQBS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2875_length_15582_cov_13.712243"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..1074
                     /locus_tag="PKN25_09210"
     CDS             265..1074
                     /locus_tag="PKN25_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV91119.1"
                     /translation="MNPRRLPSRIVCLTTETVEVLYALGEEDRIAGISGYTVIPPRAR
                     KEKPKVFAFSSGDLEKILAVAPDLVLGFSDMQADVARDLIKAGVAVHVFNQRSVDGIL
                     AMVETVGRLVGAEARALALVAELEATMDAVRARAAALPRRPRVYFEEWDEPPISAVGW
                     VSELIGIAGGEDIFAERARHPGAAQRILADPLEAARAAPDIVVGSWCGKRFRPERVAA
                     RAGWEDVPAVRDDELHEVKSALILAPGPVAIREGLPALAEIVARWAARLHA"
     gene            1327..1578
                     /locus_tag="PKN25_09215"
     CDS             1327..1578
                     /locus_tag="PKN25_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91120.1"
                     /translation="MQAPHRRLTGFVTSALAIWFGARRAWNDMVNTGIVFLMLFRYTK
                     LFDRWWDIMPKWLFFLVVALAAILVLRVLRRPCGRGVPA"
     gene            1723..2307
                     /locus_tag="PKN25_09220"
     CDS             1723..2307
                     /locus_tag="PKN25_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5610 domain-containing protein"
                     /protein_id="HNV91121.1"
                     /translation="MNTVLMSASVPAGASVQAADAARQNSAAASRLQRQAPTTESELR
                     KDLHIGILQASIQVSIRSGDQSQALLLRSAIEGINEALAPTLGPQAIQNSMDQDRSAE
                     ATAGRIVSMSTAFFEAYAARRQDDAPETVLRDFIDVIRGGFESGFGEAREILEGLGVF
                     KGAVEADVMKTRELVLEGYDRFIQERLPAAAEPQ"
     gene            2526..2798
                     /locus_tag="PKN25_09225"
     CDS             2526..2798
                     /locus_tag="PKN25_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNV91122.1"
                     /translation="MNKQELVASIAEKAGMTKADAARALDATVAAVTEALRQDETVTL
                     VGFGSFYVGERAARTGRNPRTGKNIKIKAAKVPKFRAGKGLKDSVN"
     gene            2838..2913
                     /locus_tag="PKN25_09230"
     tRNA            2838..2913
                     /locus_tag="PKN25_09230"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2871..2873,aa:Val,seq:tac)
     gene            3284..4390
                     /locus_tag="PKN25_09235"
     CDS             3284..4390
                     /locus_tag="PKN25_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HNV91123.1"
                     /translation="MSLARLFSLPAALSAGRREMALGTLGVAVGLGCTEWIGFLALGG
                     QEPWFILPMGASAVLLFCVPASPLAQPWPSLVGNLVSALIGVGCYRWLGETGTAVALA
                     GCLAVGAMFLLRCLHPPGGAVALTAVLGGPMVHELGYAFALMPVLVNTLAMLVVAFLF
                     NNLVGRRYPHLAPARAQAHGTADPLPSKRVGFRAEDLDAALASFGEVLDVDRDDLEEI
                     MVRAQMNARRRTWGALRCADIMSRDVVSVSPQAPVGEAWALLAHHRIKALPVVEEGGR
                     LVGIVSVPDFFIDRHNPEPQPVPRMRTARVVAEIMSGRVRSARPGQSLADLVGAFSDG
                     GLHHLPVADEDGRLVGMITQSDVVAALFAGEREA"
     gene            4467..4817
                     /locus_tag="PKN25_09240"
     CDS             4467..4817
                     /locus_tag="PKN25_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV91124.1"
                     /translation="MTDRTNAASGETRVEKLKSMLDFQHQAIVSRMLVKNAAGSVTLF
                     AFDKGEGLSEHTAPFDALLIGVEGEAEVRVDADIHRLGEGETLLIPAGAPHAVAPRSQ
                     FKMLLVMLKGERKA"
     gene            4910..5122
                     /locus_tag="PKN25_09245"
     CDS             4910..5122
                     /locus_tag="PKN25_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HNV91125.1"
                     /translation="MAKKFPLHPKNPERICWGCDKYCPVDALGCGNGSGRTQHPIELL
                     GDDWYTYGDWGIDPEDPATPGKKKAA"
     gene            5425..5973
                     /locus_tag="PKN25_09250"
     CDS             5425..5973
                     /locus_tag="PKN25_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNV91126.1"
                     /translation="MTKIQTPEQFAAVGQANLEAMMSLANSAIARAERLAELNLNTVR
                     SVLEDSVATTRKLAEAKDPQQLAKLQGELTKPMLDKAVAYARSVQEIATEGQQEVSNL
                     FEKQIADLNKRFHDMLEQAAKQAPAGSEGVFEAVRSAMNTANSLYDNASKAARQAAEV
                     AEANMAAATEATVKAVSAATKK"
     gene            complement(6109..6549)
                     /locus_tag="PKN25_09255"
     CDS             complement(6109..6549)
                     /locus_tag="PKN25_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNV91127.1"
                     /translation="MSLLAQLKKDSLLARKAADSVRATLLSTLIAEAEMVGKNAGNRE
                     STDDEVQQTIRKFLKNNQEAAAVIRDAGRLAVLERECAVLTAYLPPMATEAEVKAFIA
                     DAVASLAERSPKQMGAVMAALKAKYGSGFDAKQANAWVKEALAA"
     gene            6845..8245
                     /locus_tag="PKN25_09260"
     CDS             6845..8245
                     /locus_tag="PKN25_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNV91128.1"
                     /translation="MPPDPRPLPELVSFLETFPEPHILCDRDYRILAANAAYRRSWPE
                     ARSVIGRTCYDVSHHYSVPCDRAGESCPLARSLQSGQRERVLHLHHTPRGEEYVDIEL
                     SPVRDAAGEIAWFIEKMEPLHVARGVSDRRGLIGRSPAFQHMLELIARVAPSDASVLL
                     QGESGTGKELLASAVHEASRRAEGPFVVVDCSGLPETLFESEVFGHERGAFTGATARK
                     PGLVEAASGGTLFLDEVGDIPLAMQVKLLRLLETGTYRRVGSTELRRADIRLVSATHR
                     PLKRMIAEGGFRQDLYFRINTFPITVPPLREREGDLPLLIDSLLERVAPKRKLSPSPA
                     ALRVLSNYAFPGNVRELRNVLERASLMCDGELIGPEHLPEEVLHPESESAESRFASGG
                     PLAAAIPVRDPLDLEEVQRQAMLRAVRAHRGSRRELARRLGISERTLYRRLKSLGLLE
                     GQAMRGGDEEGGGAAR"
     gene            8511..9026
                     /gene="petA"
                     /locus_tag="PKN25_09265"
     CDS             8511..9026
                     /gene="petA"
                     /locus_tag="PKN25_09265"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNV91129.1"
                     /translation="MECPPAASPFFPPPARAAGAPVEADVSKLAVGDMVTVEWRGKPV
                     WILRRTPEMLATLEHNKARLADPVSGEPQQPEYATNRQRSIKPEYLVVIGICTHLGCS
                     PSEKFAIGTTEGMTADWPGGFLCPCHGSIFDLAGRVYLSQPAPTNLEIPPHKWLSDGL
                     VLIGEDDHGMA"
     gene            9231..10247
                     /locus_tag="PKN25_09270"
     CDS             9231..10247
                     /locus_tag="PKN25_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585314.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNV91130.1"
                     /translation="MNPVPALVSPCVHSPVLDPVDEPHAVSPFGERRYNAWNDHVKRR
                     WGGRVQKVSVAAGFTCPNRDGTLGSGGCTFCNNAGFTPGYLDRRDPIGEQIDAGLRFL
                     DRRYPGTARFIAYFQTYSNTYGEFARLRESYEAALAHPRICGLAIGTRPDCLPDVVLD
                     YLAELAREHIVELEIGVESCDDEVLRRINRGHDFACSEEAIRRAAARGLGVTAHLILG
                     LPGETRAGMLEAARRLSALPVAALKLHQLQLVRGTAMARDWERDPASVPLLAEDEYIA
                     LLADFVERLAPTILLQRLGSEVPPALKLAPQWNIRLSELAPRLSAELARRESWQGRRY
                     QPAS"
     gene            complement(10474..10932)
                     /locus_tag="PKN25_09275"
     CDS             complement(10474..10932)
                     /locus_tag="PKN25_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNV91131.1"
                     /translation="MSRQPLARILLLALPAVLLLACSSEPPDTHPDKPVTQRRDAFKA
                     MLRSTEPMNTMLKDRRFDADQFASLAEQLAAKRDAPWKHFGPGSNYPPTKARPAVWEK
                     PAEFERRRSEFITATDRLLAAAAARSEEQARTALAAVQDSCKACHNDFRR"
     gene            11170..13815
                     /locus_tag="PKN25_09280"
     CDS             11170..13815
                     /locus_tag="PKN25_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV91132.1"
                     /translation="MNSAVPTSAGPIPSDLPTSPAKSAPTLPATFAPTTGLPGLDAML
                     RGVARGDNIVWQVQALDDYRALVLPYAAAAQAQGRRLIYFRFAAHPPLLAGDSGAELF
                     TPRPEDGFDAFVDQVHSVIEAAGRETMYVFDCLSDLADAWLSDRMLGNFFRLTCPRLF
                     DLDTVTYFGVLRHRHARAGMDMITETTQYLLDVFRCRDRLYIRPIKVQHRSAAAMNLI
                     HAWEPGDRFRAVADSGTVAEILAGSGWQGLEDEQIRSHWQRLFDQARTFAALRAAGAP
                     ELAGEAAAFARLAGLLFPDEAGLQELIARYLNLDDLLAVRARMIGIGTIGGKAAGMLI
                     ARAIVRRDAPALHARLEAHDSFHVGSEVFDTFFVINGLWWVRRRQREADCFLQGLDGE
                     EGARARILRGRFPESTLRQFEGMLDYFGEWPFIVRSSSRLEDRYGNAFAGQYDSVFCA
                     NRGTREERLAALLDAVRQVYASTLSESALRYRQRRGLLDEHEQMALLIMRVSGTAGGR
                     WFNPHAAGVGLSVNPYPWNPRIDMKAGVVRLVAGLGTRAVDRADDDYTRLIALDAPEL
                     RPETSFGAIARHAQRRMDSLDLERGEVVSGPFAELVRGHADFPHALFTTREAEDRPAF
                     LTFDGLIAHTDFVADLRTLLRALHAAYAHPVEIEFALNFVPDGGYRINLLQCRPMQVR
                     SVEGVQGVEPPADAPRLVEACGAVIGPSRVIRPERIVLVRPAAFAALDQARRLAVTRV
                     LGRLNALADERCVLMLGPGRWGSKDPFLGIPVLFSEINHVAALCEIVAMHEHLVPDVS
                     LGTHFLNELIEADLLYFALFPRMAGNVLDEAAILRWPNRLGTLLPDDADWSTVVHVAE
                     PAQAPTLYADAPGQRVVVVQDTVAA"
     gene            13886..14341
                     /locus_tag="PKN25_09285"
     CDS             13886..14341
                     /locus_tag="PKN25_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91133.1"
                     /translation="MRLAFLSAAIAALCAAPVPVLAADDTRPQPRLQASADELPRPAD
                     EKLRAGMLQLRETVAARVSDPAMDEAAQVALAGEILRQVAMISEGRDFHSRPGRHLQW
                     LLGDISDGAGLMRDAPRLQAKRLGLLRVAETLNFYGREYDHPGWEPIRP"
     gene            14367..>15582
                     /locus_tag="PKN25_09290"
     CDS             14367..>15582
                     /locus_tag="PKN25_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNV91134.1"
                     /translation="MKPALRILAGPAARAQLRERGLAPADVLAIPGAAGGPKGLILNP
                     LDRFVFGHWLAGAGAPVHLLGASIGAWRMASACLPDADAELAEMARHYIEESYLDAEE
                     VQKARASGRPVRQPGAAAVSRAFRARLASHLGGRSDELLHHPRYRLHLFTSRGVHPLL
                     RREGRLRTLAGYAGAYAANLHSRARMGEWLERVVFSDPRDALPFALDDYAGRVCALGP
                     HNAFEALVASCSIPFWLQAVHDIPGAPPGAYWDGGITDYHLHLDYASVLRGEGNSLAA
                     AQRAGERSPGRMAGNAGSQEDGETTMGRRPRDETRAEAPRLVLYPHFQPSVVPGWLDK
                     SLRTRHRATARLDNVVLLVPDAEWIARLPNAKLPDRRDFKTYGADHAGRAVVWRRAIA
                     ESERLADEFAARV"
BASE COUNT         2287 a         5379 c         5459 g         2457 t
ORIGIN      
        1 gttttctgtt ttaaagaaaa caagaggagt gttaaagata acgcctgaag ctaggccgcc
       61 gtttcaagtc gagcatcctc caggtagccg aaaggccgtt cgcaatcggt cgccaaagcc
      121 gacgattccg gatcgcaccg gtttctgcac gtggggatcg cgaagcgggt cgctcccagc
      181 atgcaggccg tacgcgcggc gaatgggcga ggagcagacc ggggctgagc acgcgtgccc
      241 gaggggatgc gcgataatcc cgccatgaac ccacgccgcc tcccctcccg catcgtctgc
      301 ctgaccaccg agaccgtcga agtcctctac gccctgggcg aggaggaccg tatcgccggc
      361 atcagcggct acacagtcat cccgccgcgt gcgcgcaagg agaagccgaa ggtgttcgcc
      421 ttctccagcg gcgacctgga gaagatcctc gcggtcgcgc cggacctggt gctggggttc
      481 tccgacatgc aggcggacgt ggcgcgtgac ctgatcaagg ccggggtggc ggtgcatgtg
      541 ttcaaccagc gcagcgtcga cggcatcctg gcgatggtcg agacggtggg ccggctggtt
      601 ggggcggagg cgcgtgcgct ggccctggtg gccgagctcg aggcgacgat ggacgcggtg
      661 cgcgcgcggg cggcggcgct gccgcggcgg ccgcgggtgt atttcgagga gtgggacgag
      721 ccgccgatca gcgcggtggg ctgggtgtcg gagctgatcg ggatcgccgg cggtgaggac
      781 atctttgccg agcgcgcgcg ccatccgggc gcggcgcagc gcatcctcgc cgatccgctg
      841 gaggcggcgc gggcggcgcc ggatatcgtg gtgggctcgt ggtgcggcaa gcgcttccgc
      901 cccgagcgtg tggctgcacg tgcggggtgg gaggacgttc cggccgtgcg cgacgacgag
      961 ctgcacgagg tgaaatccgc gctgatcctg gcgccggggc cggtggcgat ccgcgagggg
     1021 ctgccggcgc tggcggagat cgtcgcgcgc tgggctgctc ggctgcacgc ctgaagcggc
     1081 gcggtcgcgc agcgcggcag gcagtccggt accggagtcg tccgcacacg ccttgccggg
     1141 cagcgtggcg ccgaattcgc ccacgtcatc gagaccggcc gacgaggcag tgcgttcgcc
     1201 atgtctcgct cggcatgcgg gtggcgtcct ttctcggtgc gctcgcgatg ttggcgagcg
     1261 tgttcttcct ctcccgcctg ttccggggct tctttcccga gacggtgcag gtggcgatcc
     1321 tgatcggtgc aagccccgca ccggcgcctc acgggcttcg tcaccagcgc cctggcgatc
     1381 tggttcggcg cgcgccgcgc gtggaacgac atggtcaaca ccggaatcgt gttcttaatg
     1441 ctcttccgat acaccaagct cttcgacagg tggtgggaca tcatgccgaa atggctgttc
     1501 ttcctcgtcg tcgcgctggc ggcgatcctg gtcctgcggg tgctgcggcg cccgtgcgga
     1561 cggggggtgc cggcatgagt ccgcgcgcgc tcacgctcgc cgtcggggcg atgatcatcc
     1621 cgggcggctg cggactcgcg acgtttgagc ggtcttggcg cagcgcgatg ccgggccgct
     1681 gtctagactg gaagtgagag aacaacgcca aaggagtccg ccatgaatac cgtcctgatg
     1741 agtgcttccg tccccgcggg tgcgtccgtc caggcggcgg atgccgcgcg ccagaacagt
     1801 gcggcggcgt cgcgcctgca gcgtcaggcg ccaaccaccg agtccgagct gcgcaaggat
     1861 ctccatatcg gcatcctgca ggcttcgata caggtgtcga tccgctcggg agatcaatca
     1921 caagcgctgt tgttgcgctc ggcaatcgag ggcatcaacg aggcgcttgc acccacgctg
     1981 ggtccgcagg ccatccagaa ctccatggac caggatcgtt cggccgaagc caccgccggg
     2041 cgcatcgtgt cgatgtcgac cgcgttcttc gaggcctatg ccgcgcggcg ccaggacgat
     2101 gcccctgaga ccgtgctgcg cgacttcatt gacgtgatcc gtggcggttt cgagagcggc
     2161 ttcggcgagg cgcgcgagat cctcgaaggc ttgggcgtct tcaagggcgc ggtcgaggct
     2221 gacgtgatga agacgcgcga gctggtgttg gaggggtacg accgcttcat ccaagagcgc
     2281 ctgcccgctg cggctgagcc gcagtaggtg gtctggtgga gatgcaggct ccggccatgc
     2341 tgcaccgcac gcgccggaag tctcgtcgaa tgtcccacgg cggcacttcc tgccgcgttg
     2401 acagtgctga atcgcgcgcg ctataaaatc gcgagttccg caggtcatgt cgcaacccgt
     2461 ggtgcgctcc gatcattcca aagccacggt tccacccatt gcagtacaaa accggagggg
     2521 gtcaagtgaa taaacaggaa ctggttgcaa gcattgcgga gaaggctggc atgaccaagg
     2581 cggatgcggc gcgcgcgctg gacgcgaccg ttgccgcggt gaccgaggcc ctccgtcagg
     2641 acgaaacggt caccctggtg ggcttcggct ccttctacgt gggcgagcgc gccgcgcgca
     2701 ccgggcgcaa cccgcgcacc ggcaagaaca tcaagatcaa ggcggccaag gtgccgaaat
     2761 tccgcgctgg caaagggctc aaggattctg taaactagcg gactttgctg ctccggcatc
     2821 cgcgccgggg aaagcgcggg tgcttagctc agtcggtaga gcgtcgccct tacaaggcga
     2881 atgtcggcgg ttcgaccccg tcagcaccca ccaccgaatg cgccgatttc tcggcgaaac
     2941 gaaaggcccg gaccgcgtac cgggcctttt tgtcgtggat gatcgaatgg tcgcgcgccc
     3001 tccgttgcgc acccgccgcc catcccccat ccgttcccgc tgactcccgc accctcgcac
     3061 ccatacattt tgtaatgccg cgagtcctcg ccgaatccgg gatacggttt aaaatatcat
     3121 tttttgatgt aatgaccggc tcgtgcggat gcgtgtcgat gccgcaaggg cgagcgccca
     3181 cccgcacggt gccgtgcttg ccggacagtg cagacgggag tctgccgcct gcctgaccga
     3241 ccggtgggca cccctcgttg cgcctcgccg ctggagcctg ccgatgtccc tcgcccgact
     3301 gttctccctg cctgccgcct tgtctgcggg gcggcgcgag atggcactgg ggacgctggg
     3361 ggtggcggta gggttgggtt gcaccgagtg gatcggcttc ctcgcgctgg gcgggcagga
     3421 accgtggttc atcctcccga tgggggcgtc ggcggtgctg ctgttctgtg tcccggcgag
     3481 tccgctcgcg cagccctggc cgagcctcgt cggcaacctg gtgtcggcgc tgatcggggt
     3541 aggctgctat cgctggctgg gcgagaccgg gacggccgtc gcgctcgccg gctgcctggc
     3601 ggtgggggcg atgttcctgc tgcgctgcct gcatccgccg ggcggagcgg tggcgctgac
     3661 ggcggtgctg ggtggtccga tggtgcatga actcggctac gccttcgcgc tgatgcccgt
     3721 gctcgtcaat acgctggcga tgctggtggt cgccttcctg ttcaacaacc tcgtgggacg
     3781 gcgctatccg caccttgcgc ccgcgcgtgc gcaggcgcac ggcacggccg atccgctgcc
     3841 gagcaagcgc gtcggctttc gtgcggagga cctcgacgcg gcgctggcct ctttcggcga
     3901 ggtgctcgac gtcgatcgcg acgacctcga ggagatcatg gtgcgcgcgc agatgaacgc
     3961 gcgccggcgc acctggggcg cgctgcgctg cgccgacatc atgtcgcgcg acgtggtgag
     4021 cgtgagtccg caggccccgg tgggcgaggc ctgggcgctg ctcgcgcacc accgcatcaa
     4081 ggccttgccg gtggtggagg agggcggccg gctggtcggc atcgtatcgg tgccggactt
     4141 cttcatcgat cgccacaacc ccgagccgca gccggtgccg cgcatgcgca ccgcccgcgt
     4201 ggtcgccgag atcatgagcg ggcgcgtgcg cagcgcccgc cccgggcaat cgctcgccga
     4261 cctggtgggg gccttctcgg acggcggcct gcatcacctg ccggtggccg acgaggacgg
     4321 caggctggtc ggcatgatca cccagtcgga cgtggtggcg gcgctcttcg ccggcgagcg
     4381 cgaggcttag cggacgccgg cggatcgacc cgcgcgcgca ggagtaggat tgcaatggaa
     4441 tgcctgacag aagggaggaa cccgaaatga cggatcgcac gaatgccgcg agcggcgaaa
     4501 cccgcgtcga gaagctcaag tcgatgctcg acttccagca ccaggcgatc gtgagccgca
     4561 tgctggtgaa gaatgccgcc ggcagcgtca cgctgttcgc cttcgacaag ggagagggcc
     4621 tcagcgagca caccgcgccc ttcgatgcgc tgctgatcgg tgtggagggc gaagcggaag
     4681 tgcgcgtcga cgccgacatc catcggctcg gcgagggcga aacgttgctg atccccgccg
     4741 gcgcgccgca tgcggtggcg ccgcgctcgc agttcaagat gttgctggtg atgctcaagg
     4801 gcgagcgcaa ggcctgagcg gggaggtgcc gcacccggcc gcctgccggg tgcccgcgcg
     4861 ggcagtcgag cgcgcccgga acccgggcag cgtattggag agtgtggtca tggcgaagaa
     4921 atttcccctt catccgaaga acccggagcg catctgttgg ggttgcgaca aatattgtcc
     4981 ggtcgatgcg ctcggctgcg gcaacggctc gggacgcacc cagcacccga tcgagctgct
     5041 cggcgacgac tggtacacct atggcgactg gggcatcgac ccggaggatc ccgcgacgcc
     5101 gggcaagaag aaggcggcgt gagcaggcgg gcgagggcgc tgcgctgcct ctttcatgcg
     5161 cctgaaacct cgatggaaga gcatcaggag gatcgcggcg aatgcgattg cgagcgggat
     5221 ttccaccaca tggctaacgg gtggcacagc gggatgttgc ggtgcaacaa aaaagtgttg
     5281 acggtggaat tcccaacgct ataatcggct ccattgtgcg gtgcagcaaa acgaagccgg
     5341 aatggtttcg ggcaataagg ccgcatggac cgcgagtttc tttcgcatca tcgactccac
     5401 tgcaactcac caaggaagcc agccatgacc aagatccaga cccccgagca gttcgccgcc
     5461 gtcggccaag ccaacctcga agccatgatg tcgctcgcca acagcgccat cgcccgtgcc
     5521 gagcgcctgg ccgagctcaa cctcaacacc gtgcgttcgg tgctcgagga cagcgtcgcc
     5581 accacccgca agctggccga agccaaggac ccgcagcagc tcgccaagct ccagggcgaa
     5641 ctgaccaagc cgatgctcga caaggccgtc gcctacgccc gtagcgttca ggagatcgcc
     5701 accgagggtc agcaggaagt gtcgaacctg ttcgagaagc agatcgccga cctcaacaag
     5761 cgcttccacg acatgctcga gcaggccgcc aagcaggcgc ccgccggttc ggaaggcgtg
     5821 ttcgaggccg tccgctcggc gatgaacacc gccaacagcc tgtacgacaa cgccagcaag
     5881 gccgcccgcc aagccgccga agtcgccgaa gccaacatgg ccgccgcgac cgaagccacc
     5941 gtcaaggccg tgagcgccgc gaccaagaag tgatcgcagc gcgcggagag gcggtcgctt
     6001 ctccacgcaa cagaaaaggc cgcccggcat tgccgggcgg cctttcttac atggtgcccg
     6061 gctcgccgca tggaagccgc agcggccggc cgggcctgcc gacgggcctc aagcggccag
     6121 ggcttccttg acccaggcgt tcgcctgctt ggcgtcgaag ccgctgccgt atttggcctt
     6181 cagcgcagcc atcaccgcgc ccatctgctt gggcgagcgc tcggccaggc tggcgaccgc
     6241 gtcggcgatg aaggccttca cctcggcctc ggtggccatc ggcggcaggt aggccgtcag
     6301 caccgcgcac tcgcgctcga gcacggccag gcggccggcg tccctgatca cggccgcggc
     6361 ttcctggttg ttcttgagga acttgcggat cgtctgctgc acctcgtcgt cggtgctctc
     6421 gcggttgccg gcgttcttgc cgaccatctc ggcctcggcg atcagggtgg acagcagggt
     6481 ggcgcgcacg ctgtcggcag ccttgcgcgc gagcagggag tctttcttga gttgggccag
     6541 caggctcatc ggggtctccg gtggaatcga gcgggggagg cgtgcgacgc aggcctccgg
     6601 tcacggagcg gcgtgcagga cgccggtcgg gggccgagtc taccgcagcc cgtaccgcca
     6661 cagcgcccgg atcgcgcctc gcagcctgcg gttcccgctg acgcggcgca cgttgccgaa
     6721 gcacgcctgc cgcagtcgcg ccgcccgctc gcgctgccca ggcctggctc cagaggaggc
     6781 gattgacggc gccgctcccg gggacgcaga ctgctggcag accttccgcc agatctgaca
     6841 ggacatgccg cccgaccccc gcccgctgcc cgagctggtc tccttcctcg agaccttccc
     6901 cgaaccgcac atcctgtgcg atcgcgacta ccgcatcctc gccgccaacg ccgcctaccg
     6961 acgctcgtgg cccgaggcgc gcagcgtgat cgggcgcacc tgctacgacg tgtcgcacca
     7021 ctacagcgtg ccctgcgatc gcgccggaga atcctgcccg ctcgcgcgca gcctgcagtc
     7081 gggacagcgc gagcgcgtgc tgcaccttca ccacacgccg cgcggcgagg agtacgtcga
     7141 catcgaactc tcgcccgtgc gcgacgccgc tggcgagatc gcctggttca tcgagaagat
     7201 ggagccgctg cacgtcgccc gcggggtgtc cgaccgccgc ggcctgatcg gacgttcgcc
     7261 ggccttccag cacatgctcg aactcatcgc gcgggtcgcg ccctcggacg cgagcgtgct
     7321 gctgcagggc gagtccggca ccggcaagga gctgctcgcg agcgcggtgc acgaggcgag
     7381 ccggcgcgcc gaggggccct tcgtggtggt ggactgctct ggcctgcccg agaccctgtt
     7441 cgagagcgag gtcttcggtc acgagcgcgg cgccttcacc ggcgccacgg cgcgcaagcc
     7501 cggcctggtc gaggcggcca gtggcggcac cctttttctc gacgaggtcg gcgacattcc
     7561 gctcgccatg caggtcaagc tgctgcgcct gctcgagacc ggcacctacc gccgcgtcgg
     7621 ctccaccgag ttgcgtcgtg ccgacatccg cctggtctcg gccacccacc gtccgctcaa
     7681 gcgcatgatc gccgagggcg gcttccgtca ggacctgtac ttccgcatca acactttccc
     7741 catcaccgta ccgccgctgc gcgagcgtga gggcgacctg ccgctgctga tcgactccct
     7801 gctcgaacgt gtcgcgccga agcgcaagct gtcgccgagt ccggccgcgc tgcgcgtgct
     7861 gagcaactac gccttcccgg ggaacgtgcg cgagctgcgc aacgtgctcg agcgtgccag
     7921 tctgatgtgc gacggcgagc tgatcgggcc cgagcatctg cccgaggagg tgctgcatcc
     7981 cgagagcgag agcgccgagt cacgatttgc cagtggaggt ccgttggccg cagcgatccc
     8041 ggtgcgcgat ccgctggatc tcgaggaggt gcagcgccag gccatgttgc gcgccgtgcg
     8101 cgcgcatcgt ggcagtcggc gcgagctggc tcgccggctg gggatcagcg agcgcacgct
     8161 gtatcggcgg ctgaagtcac tcggcctgct cgagggccag gcgatgcgcg gcggggatga
     8221 ggagggtggg ggggcggcgc gctgagtggg ggggcgcggg aagcagcacc ggatcgcggc
     8281 acttggtcgc ggaggtgccg ggttgctgtc agctgtggca gtaccatcga gggggcggca
     8341 gcctttggca gtatcaaggc gcccaaatac gacaactatg cccgacagac cggccttttc
     8401 tggagctggt acgccgattg ctttgtagag cccagagcga agcaggccgc gtcgtgatcg
     8461 ctgcatgatc gtcccccctc gacggtcttg cccctggcag cgcagttccg atggagtgcc
     8521 ccccggccgc ttcgcccttt tttccgccgc ccgcccgggc cgccggcgcg ccggtcgagg
     8581 ccgacgtctc caaacttgca gtcggcgaca tggtgaccgt ggagtggcgt ggcaagccgg
     8641 tgtggatcct gcgccgcacg cccgagatgc tcgccacgct cgagcacaat aaggcccgcc
     8701 tcgctgaccc tgtctcgggc gaaccgcagc agcccgagta cgccaccaac cggcagcgct
     8761 cgatcaagcc ggaatacctc gttgtgatcg gcatctgcac gcacctgggc tgctcgccgt
     8821 ccgagaagtt cgccatcggc accaccgagg gcatgaccgc cgactggccc ggcggcttcc
     8881 tgtgcccctg ccacggctcg atcttcgacc tcgccggccg cgtctatctc agccagccgg
     8941 cccccaccaa cctcgagatc ccgccgcaca agtggctctc cgacggcctc gtgctgatcg
     9001 gcgaggacga ccacggcatg gcatgaacgg ggctgccgct cctgcagggc ttccgaagcg
     9061 cggcacttcc gcgcaagcca ttctgctccg cggggcgatt tctccagcgg cctgcgtttg
     9121 cgcacggtca agggctgcgc gggcgatcgg cggcacactc gcgcctcggc aacttcgcca
     9181 gcggcatccg gctgcgccgc tttcaccgtc catccacttt cgctccccgc atgaacccgg
     9241 ttcccgcact cgtctctccc tgcgttcact ctccggtgct ggatccggtc gatgagccgc
     9301 acgcggtttc gcccttcggc gagcgccgct acaacgcctg gaacgaccac gtgaagcggc
     9361 gctggggtgg gcgggtgcag aaggtctcgg tggcggccgg cttcacctgc ccgaaccgcg
     9421 acggcaccct gggcagcggc ggctgcacct tctgcaacaa cgccggcttc acccccggct
     9481 acctggatcg ccgcgacccg atcggcgagc agatcgacgc cgggctgcgc ttcctcgacc
     9541 ggcgctatcc cggcaccgcg cgcttcatcg cctacttcca gacctacagc aacacctacg
     9601 gcgagttcgc gcgcctgcgc gagagctacg aggccgcgct cgcgcacccg cgcatctgcg
     9661 ggctcgcgat cggcacgcgg ccggactgcc tgcccgacgt agtactcgac tacctggccg
     9721 agctcgcgcg cgagcacatc gtggagctcg agatcggcgt ggagtcctgc gacgacgaag
     9781 tcctgcgccg gatcaaccgc ggccacgatt tcgcctgcag cgaggaggcg atccggcgtg
     9841 ccgcggcgcg cggcctgggg gtgaccgccc acctgatcct cggcctgccc ggcgagacgc
     9901 gcgccggcat gctggaagcc gcgcgccgcc tgtccgcgct cccggtggcg gcgctcaagc
     9961 tgcatcagct gcagctggta aggggcaccg cgatggcacg cgactgggag cgcgatcccg
    10021 cgagcgtacc cttgctcgcc gaggacgagt acatcgcgct gctggccgac ttcgtcgagc
    10081 ggcttgcgcc cacgatcctg ctccagcgcc tgggcagcga ggttcccccg gcgttgaagc
    10141 tggcgccgca gtggaacatc cgcctctccg agctcgcccc ccggctgagt gcagagctgg
    10201 cgcggaggga aagctggcaa ggccgccgct accaacccgc ctcctgagtg tcggcggcag
    10261 ggccacggac gggggcgacg cgagtcgcga tcggagcgca tgaggttcgg gaacgcccgg
    10321 cgggatctcg tacgctggaa tcgcacttgc gcgagaccgc ggcgtctccg gaagcgcacc
    10381 gtgggagcga cgcgagtcgc gatcgggcgc aggagatgcg acgacgcccg gtgggaccgc
    10441 gggttccgca atcgcgcctg cagtcttgcc gcctcagcgc ctgaagtcgt tgtggcaggc
    10501 cttgcagctg tcctgaacgg ctgcgagggc ggtgcgcgcc tgttcctccg agcgcgccgc
    10561 agcggcggcg aggaggcggt cggtggcggt gatgaactcg ctgcgacggc gctcgaactc
    10621 ggcgggcttt tcccagacgg ccgggcgggc cttggtgggg gggtaattgc tgccggggcc
    10681 gaagtgcttc cacggcgcgt cgcgcttcgc ggcgagctgc tcggcgaggc tggcgaactg
    10741 gtcggcgtcg aaacgccggt ccttgagcat ggtgttcatg ggctcggtgc tgcgcagcat
    10801 ggccttgaag gcgtcgcggc gctgggtgac cggcttgtcc gggtgggtat cgggcggctc
    10861 cgacgaacag gcgagcagca ggacggcggg cagggcgagg agcaggatgc gggcgagggg
    10921 ctgtcgggac atttcgggcg tgaggagtcg ggagtgatgc gtcgggtgcc ggtctaggct
    10981 cttgcggaac tcgaaggcga ggcgggaagg gtcggattcc cggcacgagg gcgcgcggcc
    11041 gccggccgat ggtgcagcag cgcaatcgtt cgacggttgg caaggcaaga agaatgccac
    11101 gcggcgggaa agttcccgcg gggcttgcgc gcataatcgc gctcgcaggc cttgtccgag
    11161 gagctcctga tgaattctgc cgttccgacg tcggccggac ccatcccgtc cgatcttccc
    11221 acgtctcccg ccaagtccgc tcccacgctc cccgccacct tcgccccgac caccggcctg
    11281 cccggcctgg atgcgatgct gcgcggcgtg gcgcgcggcg acaacatcgt ctggcaggtg
    11341 caggcgctgg acgactaccg cgcgctggtg ctgccctatg cggccgcggc gcaggcgcag
    11401 ggacggcggc tgatctattt ccgtttcgcc gcgcatcctc cgctgctcgc cggggacagc
    11461 ggcgccgagc tgttcacccc gcgtcccgag gacggcttcg acgccttcgt cgatcaggtg
    11521 cattcggtga tcgaggccgc cggtcgcgag accatgtatg tcttcgactg cctttcggac
    11581 ctcgccgacg cctggctgtc cgaccgcatg ctcggcaact tctttcgcct cacctgcccg
    11641 cggctcttcg acctcgacac ggtgacctac ttcggcgtgc tgcgccatcg acatgcgcgc
    11701 gcgggcatgg acatgatcac cgagacgacg cagtacctgc tcgacgtgtt ccgctgccgc
    11761 gatcggctct acatccggcc gatcaaggtg cagcaccgct cggccgcggc gatgaacctg
    11821 atccacgcct gggagccggg cgatcgcttc cgcgcggtcg ccgacagcgg cacggtggcc
    11881 gagatcctcg cgggctcggg ctggcagggg ctggaggacg aacagatccg cagccactgg
    11941 cagcgcctct tcgatcaggc gcgaaccttc gccgccctgc gcgcggccgg ggcgcccgag
    12001 ctggccggcg aggccgccgc cttcgcccgc ctggcgggcc tgctgtttcc cgacgaggcg
    12061 ggcctgcagg agctgatcgc gcgctacctg aacctcgacg acctgctcgc ggtgcgcgcg
    12121 cgcatgatcg gcatcggcac catcggcggc aaggccgcgg gcatgctgat cgcgcgcgcg
    12181 atcgtgcgcc gcgacgcgcc tgcgctgcac gcccgcctcg aggcgcacga ctccttccat
    12241 gtcggcagcg aggtcttcga taccttcttc gtgatcaacg ggctgtggtg ggtgcgccgc
    12301 cgccagcgcg aggccgactg cttcctccag ggcctcgacg gagaggaggg cgcccgcgca
    12361 cgcatcctgc gcggccgctt ccccgaatcc accctgcgcc agttcgaggg catgctggat
    12421 tattttggcg agtggccttt catcgtgcgc tccagttcgc gcctggagga ccgctacggc
    12481 aacgccttcg ccggccagta cgacagcgtg ttctgcgcca accgcggcac gcgcgaggag
    12541 cgcctcgccg cgctgctcga cgccgttcgc caggtgtatg cgagcacgct gtcggagtcg
    12601 gcgctgcgct accgccagcg ccgcggcctg ctcgacgagc acgagcagat ggcgctgctg
    12661 atcatgcgcg tctcgggcac cgccggcggg cgctggttca acccgcacgc ggccggcgtc
    12721 gggctgtcgg tcaatcccta cccgtggaac ccgcgcatcg acatgaaggc gggcgtggtg
    12781 cgcctggtcg ccggtctggg cacgcgtgcg gtggatcgcg ccgacgacga ctacacccgc
    12841 ctgatcgcgc tcgatgcgcc cgagctgcgc ccggagacca gcttcggtgc gatcgcgcgc
    12901 catgcccagc gccgcatgga cagcctcgac ctggagcgcg gcgaggtggt ctcgggcccc
    12961 ttcgccgagc tggtgcgcgg ccatgcggat tttccgcacg cgctgttcac gacgcgcgag
    13021 gccgaggacc gtccggcctt tctgaccttc gacgggctga tcgcccacac cgatttcgtc
    13081 gccgacctgc gcacgctgct gcgcgcgctg cacgcggcct acgcgcatcc ggtggagatc
    13141 gagttcgcgc tcaatttcgt tcccgacggc ggctaccgca tcaacctgct gcagtgccgg
    13201 ccgatgcagg tgcgcagcgt cgagggcgtg cagggcgtgg agccgccggc cgacgcgccg
    13261 cgcctggtgg aagcgtgcgg tgcagtgatc ggaccgagcc gggtgattcg ccccgagcgc
    13321 atcgtgctgg tgcgcccggc cgccttcgcc gcgctcgacc aggcccgccg cctggcggtg
    13381 acccgcgtgc tcggccgcct caacgcgctt gccgacgaac gttgcgtgct gatgctcggc
    13441 cccgggcgct ggggctcgaa ggaccccttt ctcggcatcc cggtgctgtt ctccgagatc
    13501 aatcacgtcg ccgcgctgtg cgagatcgtc gccatgcacg agcacctggt gcccgacgtc
    13561 tcgctcggca cccacttcct caacgagctg atcgaggccg acctgctgta cttcgcgctg
    13621 ttcccccgga tggcgggcaa cgtactcgac gaggcggcga tcctgcgttg gcccaaccgg
    13681 ctcggcacgc tgctgccgga cgacgcggac tggagcacgg tggtgcacgt ggccgagccc
    13741 gcgcaggcac ccacgctgta cgccgatgcg cccgggcaac gtgtcgtcgt ggtgcaggac
    13801 acggtggcgg cttgattcag ttcaagcgct ttcaggggcc gcccgggtag gatggcgacc
    13861 gttcgcatcc aaccggagat cgtgcatgcg cctcgccttc ctgagcgccg ccatcgcggc
    13921 gctgtgtgcc gcccctgttc ccgttctcgc cgccgacgac acacgcccgc aacccaggct
    13981 gcaggcatcg gccgacgagc tgccgcgccc cgccgacgag aagctgcgcg ccggcatgct
    14041 gcaactgcgc gagaccgtcg ccgcacgcgt gtccgacccg gccatggacg aggctgcgca
    14101 ggtcgcgctc gccggcgaga tcctgcgcca ggtcgccatg atcagcgagg ggcgcgactt
    14161 ccacagccgt cccggccgcc acctgcaatg gctgctcggc gacatcagcg atggcgccgg
    14221 gctcatgcgc gatgcgccgc gcctgcaagc caagcggctc ggcctgctgc gggtcgccga
    14281 gacgctgaac ttctacggtc gcgagtacga ccacccgggg tgggagccga tcaggccgtg
    14341 aggggccgcg cggaggcgac ggcgcgatga agccggcgct gcgcatcctg gctggtccgg
    14401 cggcgcgtgc gcagttgcgc gagcgcggtt tggcgcccgc cgacgtcctt gcgatccccg
    14461 gcgcggccgg cggccccaag ggcctcatcc tcaatccgct cgatcgcttc gtcttcggcc
    14521 attggctggc gggcgcgggt gcgccggtgc acctgctcgg cgcctcgatc ggcgcctggc
    14581 gcatggccag cgcatgcctg cccgatgcgg acgccgagct cgccgagatg gcgcgtcatt
    14641 acatcgagga gagctatctc gacgccgaag aggtgcagaa ggcgcgcgcc agcggccgcc
    14701 cggtgcgcca acccggcgcg gcggcggtca gccgcgcctt ccgcgcgcgc ctcgccagcc
    14761 atctgggcgg gcgcagcgac gagctgctgc accacccccg atacaggctg cacctcttca
    14821 ccagccgcgg cgtgcatccc ctgctgcggc gcgagggccg gctgcgcacg ctcgccggct
    14881 atgcgggcgc ctacgctgcc aacctgcaca gccgcgcccg catgggcgaa tggctcgagc
    14941 gcgtggtgtt ctcggatccg cgcgacgcgc tgcccttcgc cctggacgac tacgccgggc
    15001 gcgtctgcgc gctcggtccg cacaacgcct tcgaggcctt ggtggcgagc tgctcgatcc
    15061 cgttctggct gcaggcggtg cacgacattc ccggcgcccc gccgggtgcc tactgggatg
    15121 gcggcatcac cgactaccat ctgcacctgg attacgcctc ggtgctgcgc ggggagggga
    15181 acagccttgc ggcagcgcaa cgcgcgggcg agcgcagtcc cggcaggatg gccgggaatg
    15241 cgggtagcca agaggacggc gagacgacga tggggcggcg gccgcgcgac gaaacccgtg
    15301 cagaggcgcc ccggctcgtg ctctacccgc atttccagcc cagcgtcgtt ccgggttggc
    15361 tggacaagtc gctgcgcacg cgccatcgcg ctaccgcgcg cctggacaac gtggtgctgc
    15421 tggtgccgga cgccgagtgg atcgcccgcc tgcccaacgc caagctgccc gatcggcgcg
    15481 acttcaagac ctatggcgcc gaccacgcag gacgcgcggt ggtgtggcgg cgcgcgatcg
    15541 cggaaagcga gcggctcgcc gacgagttcg ccgcgcgcgt cg
//
LOCUS       DAOQBS010000070        15040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3024_length_15040_cov_15.925993, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000070 DAOQBS010000000
VERSION     DAOQBS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 15040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15040
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3024_length_15040_cov_15.925993"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1232
                     /locus_tag="PKN25_09295"
     CDS             3..1232
                     /locus_tag="PKN25_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNV91135.1"
                     /translation="MTCGSVDDGKSTLIGRLLYDTKTILADTLSAIAKTSEKRGLGAV
                     DLSLLTDGLQAEREQGITIDVAYRYFSTGRRKYIIADAPGHEQYTRNMVTAASTANLA
                     IILIDARKGVLTQTRRHSYLASLVGIPHLVVAVNKMDLVDYDQAVFERIKGEYLEFAA
                     KLGIRDVRFIPISALEGDMIVDRGERLSWYDGPTLLDILENAPAAHTEQAEDFRFPVQ
                     FVCRPQESANPELHDYRGFMGRVESGEVAVGDAVTVLPSGASSRVRKIELGGVALPRA
                     IHEQSVTLLLEDEIDISRGDLIVKSAEAREPVKQIEATVCWLSETPLSPARTYLVRHT
                     TREVKAKLAKIDYRLDVNTLEQEATTGLAMNDIARVTLKLAQPIIADRYTENRATGAF
                     IIIDESTNNTVGAGMIG"
     gene            1472..2209
                     /locus_tag="PKN25_09300"
     CDS             1472..2209
                     /locus_tag="PKN25_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HNV91136.1"
                     /translation="MLKRITLVTVATLVLAGCAATHVAISKRDLDVQTKMSDTVFLDP
                     VGPDKRTIFLQIRNTSDKPDLAVNDEIAAALQTKGYTLLQDPEAAHYVLQANILQVGK
                     SAPTAIEAAIDQGYGVAASGGLVLGAGAAYAGGASGRGIAAAGLIGSLAEGIAGAAVK
                     DVYFSMITDLQIKERIRGAGKARVSSNHSLRQGNSGGSQVSYDEESSYKTYQTRIGST
                     ANKVNLAFEEALPELKKGLINSISGLF"
     gene            2308..2709
                     /locus_tag="PKN25_09305"
     CDS             2308..2709
                     /locus_tag="PKN25_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNV91137.1"
                     /translation="MTDRNPSDTARTRLDKWLWAARFFKHRTGATEAVDGGKVKLNGM
                     AAKPARELKVGDRLDITIGEDTRTVIVRAIADKRGSAAVAQALYEETAESIEARERAR
                     ELRKFAAAPGADLHGRPTKRDRRRMDRFGGC"
     gene            complement(2778..3653)
                     /locus_tag="PKN25_09310"
     CDS             complement(2778..3653)
                     /locus_tag="PKN25_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNV91138.1"
                     /translation="MLIRIVSILFPLFAITALGYFVGRRMKPDLSHANKLNMDVFVPA
                     LVFGALVGKDFRLGEYLPLLFATVVIIVGSGVAGWLVARAAGIATKTLVPPMMFNNCG
                     NLGLPLAVLAFGESALAPAVVMFVVSNVIHFSYGAWLLDHHTKLTTVWRSPSILATIA
                     GLAVGFLGIEVWPPLLMSIEMVGDISIPLMLFALGVRLADSRISAVGFGLFGAVLRPV
                     VGMALAWALLQMLELPPREQALLLVFGALPPAVLNYMFAERYDQEPDKVASMVLIGNL
                     AAVVFLPIALALVLD"
     gene            complement(3772..5109)
                     /gene="hslU"
                     /locus_tag="PKN25_09315"
     CDS             complement(3772..5109)
                     /gene="hslU"
                     /locus_tag="PKN25_09315"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNV91139.1"
                     /translation="MTQMTPPEIVSELDKHIVGQDKAKKAVAIALRNRWRRAQVEEPL
                     RSEITPKNILMIGPTGVGKTEIARRLARLANAPFIKIEATKFTEVGYVGRDVDTIIRD
                     LMEIAVKDGRERAMKSVRDRALDAAEDRVLDALLPPARPVGFNAEPEPAQDSSTRQKF
                     RKKLREGELDDKEIEIEVAAPSMQAEIFAPPGMEELTQQIQGMFQNLGGGKKKQRKLQ
                     IREAMKLLADEEAARLINDEEVKLEAVRAVEQNGIVFLDEVDKIAARSDVQGADVSRQ
                     GVQRDLLPLVEGTTISTKYGMIKTDHILFIASGAFHLSKPSDLIPELQGRFPIRVELE
                     SLSVEDFARILTSTDACLTRQYEALLATDGVKLEFADDGIRRLAEIAYQVNEKTENIG
                     ARRLYTVMEKLLEEVSFEAGRSSAEQTVVVDAAYVDSRLVMLAQREDLARYVL"
     gene            complement(5120..5656)
                     /gene="hslV"
                     /locus_tag="PKN25_09320"
     CDS             complement(5120..5656)
                     /gene="hslV"
                     /locus_tag="PKN25_09320"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNV91140.1"
                     /translation="MEQYRGTTILSVRRGNRVALGGDGQVTLGNIVIKASARKVRTLY
                     NGQILAGFAGGTADAFTLFERFEAKLDKHQGNLLRSAVELAKDWRTDRMLRRLEAMLA
                     VADREHSLVITGNGDVLEPEQGIVAIGSGGAYAQSAARALLENTELEPREIVSKALSI
                     AGDLCIYTNHHHTIEVLD"
     gene            complement(5700..6881)
                     /locus_tag="PKN25_09325"
     CDS             complement(5700..6881)
                     /locus_tag="PKN25_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter"
                     /protein_id="HNV91141.1"
                     /translation="MALPFFGKKPVSPAPGAPRDARAGPGADALRDERPPPTELSALD
                     FTGTDHGRALARVAGQVEVRECGSGIGAAFEEAAVLYANGSDDDALAVLEAAVGAEGG
                     EGGEGAWLMLLDLYRLRGERQRFESRVLDYATRFERSPPPWEDLSRVAGKAAGTELPL
                     VNLSGQLSAAAERQFAQLAKIARRSGGVRIDVAKVRGVDDAGCAVWRGLLAGLAAERL
                     QVRLVNVRALADILSQAVSPGKAQARDTWLMLLELLQYAGDQARFEDVAVDYAVTFEE
                     SPPSWEGRGDAPTARAAQADEAQPTDPEAFAFEGELCGAANDTLRRLAAFAVERREVR
                     IDCRRLRRLDFVCAGTLFNILATLQAQGKLVCLRDVNAMVAGLLRVMSVDKVAHVTLR
                     H"
     gene            7041..7493
                     /gene="ybgC"
                     /locus_tag="PKN25_09330"
     CDS             7041..7493
                     /gene="ybgC"
                     /locus_tag="PKN25_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HNV91142.1"
                     /translation="MQPKTVSDAGGESLPASSRFSLSVRVYYEDTDAGGVVYYANYLK
                     FCERTRTEWLRALGVAQHALLDEQGVGFVVRSVQADYLAPARLDDALEVSARIASRRR
                     ASLVFDHEIRRGGELLFTAQVLVACVDMRRMKPAALPASLHALLESPA"
     gene            7490..8158
                     /gene="tolQ"
                     /locus_tag="PKN25_09335"
     CDS             7490..8158
                     /gene="tolQ"
                     /locus_tag="PKN25_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HNV91143.1"
                     /translation="MTVTHDLSILSLISQASVLVQLVMALLASLSLVSWYWIFRKAFQ
                     IRQARAQTDEFERDFWSGGDLNTLYESAGRHQSEGMERIFEAGYREFTKLRGKGHDHG
                     ATLDGARRAMRATYQREIDDLEAHLAFLASVGSVSPYIGLLGTVWGIMNSFRGLSSVG
                     AATLAQVAPGIAEALVATAIGLFAAIPAVVAYNRFAHDIDRLGIRFESFMEEFSNILQ
                     RNLR"
     gene            8178..8594
                     /gene="tolR"
                     /locus_tag="PKN25_09340"
     CDS             8178..8594
                     /gene="tolR"
                     /locus_tag="PKN25_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HNV91144.1"
                     /translation="MRQRRLMNQINVVPYIDVMLVLLVIFMVTAPMIQQGTINVPSAG
                     AGTPPPQSEAIFIEVKADGAYALRASNSGSSRPVRRDQLSTALKDALAKNPEQPFLVA
                     ADSKLDYQKVIDVLETARDAGVRKISLVTQSATSQK"
     gene            8591..9565
                     /locus_tag="PKN25_09345"
     CDS             8591..9565
                     /locus_tag="PKN25_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNV91145.1"
                     /translation="MRDRPAPHRREPPGKWQSLGLTVAVHLGLFLFLFFGIRWQSPPP
                     AALEVGLASAPERSAPAAARPQPAPEPRPEPKPEPKPEPKPEPKPEPKPEPKPEPRPE
                     PKPEPRPEPPKPQAKPEIVAKAPEKKPEPPKPEPPKPEPKPQPKPEPKPEPKPEPKPQ
                     PKPEPKPEPKPQAKPEPKPQPKPDTKPIDDYMAQRLAQETQRAEQARLSSLMAQEGAR
                     AAAAGPSRQGPPGGDIDKYRAAIAAKVRGNLLRPPGLSGNPEAVFEVDQLPSGEVLNV
                     RLKRSSGVPALDEAIERAIRRSSPLPLPDNKNQFERTLELKFRPLADD"
     gene            9606..10883
                     /gene="tolB"
                     /locus_tag="PKN25_09350"
     CDS             9606..10883
                     /gene="tolB"
                     /locus_tag="PKN25_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNV91146.1"
                     /translation="MIKTLHAFRLFLAGLLASLALGAHAQLSIEITGTGASRFPVIIP
                     IFENEGRLPQSVTDVVRADLERSGLFTLIDTGPLPMPESVAPDLAAVRSRGADAVLTG
                     SLIPMADGRYDVRFRLFDAQKQQELGAMSLPMSPAQNRLVGHRIADFVYEKLTGLPGY
                     FATRIAYVIKSGPNFELQIADADGMNAATALRSREPIISPSWSPDGQRLAYVSFEQKK
                     PIVYVHTLATGQRRVVANFKGSNSAPSWSPDGSQLAVVLTKDGLSQLYAIGADGSGVR
                     RLAQSSGIDTEPFWGADGHIYFTSDRGGSPQIYRIPASGGSAQRVSFEGNYNVTPRLS
                     FDGKLLAFITRNAGRFQVAVQDVATRQTTILTDSARDESPSFAPNGRMILYATDTGGR
                     GVLSAVSTDGRIRQRLTVQAADVREPAWGPQIR"
     gene            10952..11464
                     /gene="pal"
                     /locus_tag="PKN25_09355"
     CDS             10952..11464
                     /gene="pal"
                     /locus_tag="PKN25_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNV91147.1"
                     /translation="MKKLVLPALLALTLAACSSTGPENGAASVQDRGTGVATVTAGSA
                     KGSGIAALTDPNNILSKRSVFFDFDSFVIKQEARPLIEAHARFLVQNPQMNMLVQGNT
                     DERGSREYNLALGQKRADVVKQALMLLGAKESQIESVSLGEEKPRCEDRTENCYAENR
                     RGDMLYTGEF"
     gene            11477..12274
                     /gene="ybgF"
                     /locus_tag="PKN25_09360"
     CDS             11477..12274
                     /gene="ybgF"
                     /locus_tag="PKN25_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNV91148.1"
                     /translation="MKRLLPLAAAFVLIPSAPAHAQLFGGDTEARNQIFQLRQDVQGQ
                     IETTSRGQLELAMQNEQMRAELARLRGQIELLTNEVETLKQRQKDFYVDLDARLRRYE
                     SGAAGAASAAPASGDAAPAAPAAAAAPPQAPAPAPAPASANTESADYQGALALLKNGK
                     HKDAQTAFERFITRYPAGNFTAGAHFWAGNAALQARDVASANRHFKTVMDKYPKENVA
                     PDAMLGLANSQQAMNDAAGAKRTLQSLVERYPQSNAAQVARQRLSAR"
     gene            12278..12949
                     /gene="queE"
                     /locus_tag="PKN25_09365"
     CDS             12278..12949
                     /gene="queE"
                     /locus_tag="PKN25_09365"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNV91149.1"
                     /translation="MSAVETAPATAVRLRITEIFASLQGESTRVGLPTVFVRLTGCPL
                     RCTWCDTAYAFSGGETRTLDDILAEVASHGLRHVCVTGGEPLAQKGCLPLLAALCDAG
                     HDVSLETSGALDIAGVDPRVSRIVDLKAPGSGELARNRWENLALLCAHDELKFVLADA
                     ADYAWAKQQIAEHRLAERCGVLLSPVAGALDPAELAGWIVRDRLPVRFQLQLHKILWN
                     DARGR"
     gene            13020..13721
                     /gene="queC"
                     /locus_tag="PKN25_09370"
     CDS             13020..13721
                     /gene="queC"
                     /locus_tag="PKN25_09370"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNV91150.1"
                     /translation="MTSLHTPPRAVVLLSGGLDSATCLAIARDMGFETYALSVAYGQR
                     HAAELAASKRVAQALGAREHRTASVELGQFGGSALTDPGIAVPLDQDNTGIPVTYVPA
                     RNTVMLSIALAWAEVLGANDIFVGVNAVDYSGYPDCRPVFIEAFEHMARLATKVGVEG
                     AQLHIHAPLIELSKADIIRRGTALGVDYGLTVTCYQADDDGRACGRCEACRLRAAGFK
                     AAGVPDPTPYQPRSA"
     gene            13736..14842
                     /locus_tag="PKN25_09375"
     CDS             13736..14842
                     /locus_tag="PKN25_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNV91151.1"
                     /translation="MDHALVSASTIVWLGFAIGAAFGASSQRTGFCTMGAVSDAVNIG
                     DWTRMRMWALAVALATLGTGALHALGLFDPAKTLYTGARLPWLSHIVGGLTFGVGMTL
                     ASGCAGKTLIRIGGGNLKSVVVFVFLAIGASMTLRGLTGVWRVEFLDPVALQLPHGQT
                     LPAFLAAAGMEAGKAALAASALAGGGLLAWALASRDARHPEVLLGGAIVGLAVVAGWY
                     VTGHLGHIAEHPDTLEEVFLATNSGRAESLSFVAPQAFTLELLLLWSDANRQMSFGIA
                     SALGVIAGAAAMALATGSFRIESFRDPADLVRHIAGGLLMGFGGVTALGCTLGQGVSG
                     LSTLALGAAITTAAIIAGAVATMKLQYWLPTRAG"
     gene            14964..15039
                     /locus_tag="PKN25_09380"
     tRNA            14964..15039
                     /locus_tag="PKN25_09380"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14997..14999,aa:Lys,seq:ttt)
BASE COUNT         2532 a         5567 c         4746 g         2195 t
ORIGIN      
        1 ttctgacctg cggcagcgtc gatgacggca agagcaccct gatcggccgc ctgctttacg
       61 acaccaagac catcctcgcc gacacgctgt cggccatcgc caagacttcg gaaaagcgcg
      121 gccttggcgc ggtcgacctc tcgctgctca ccgacggcct gcaggccgag cgcgagcagg
      181 gcatcaccat cgacgtcgcc tatcgctact tctccaccgg ccggcgcaag tacatcatcg
      241 ccgacgcccc cggccacgag cagtacaccc gcaacatggt gaccgccgcg tccaccgcca
      301 acctggcgat catcctcatc gacgcgcgca agggcgtgct gacccagacc cgccgccact
      361 cctacctcgc cagcctggtc ggcattcccc atctggtggt cgcggtcaac aagatggacc
      421 tggtcgacta cgaccaggcg gtgttcgagc gcatcaaggg ggaatacctc gaattcgccg
      481 ccaagctcgg catccgggac gtgcgcttca tcccgatctc ggcgctggag ggcgacatga
      541 tcgtcgaccg cggcgagcgc ctgtcctggt acgacggccc gaccctgctc gacatcctcg
      601 agaacgcccc cgccgcccac accgaacagg ccgaggactt ccgcttcccg gtgcagttcg
      661 tgtgccgacc gcaagagtcc gccaaccccg aactgcacga ctatcgcggc ttcatgggcc
      721 gggtcgaatc gggcgaggtc gcggtgggcg acgcggtcac cgtactgccc tcgggcgcca
      781 gcagccgggt caggaagatc gagctcggcg gcgtcgcgct gccgcgcgcc atccacgagc
      841 agtcggtgac cctgctgctc gaagacgaga tcgacatctc gcgcggcgac ctgatcgtga
      901 aatccgccga ggcgcgggag ccggtcaagc agatcgaagc cacggtgtgc tggctgtccg
      961 agaccccgct gtcgcctgca cgcacctacc tggtacgcca caccacgcgc gaggtgaagg
     1021 ccaagctcgc gaagatcgac taccgcctgg acgtgaatac cctggaacag gaggccacca
     1081 ccgggctggc gatgaacgac atcgcccgcg tcacgctcaa gctcgcccag ccgatcattg
     1141 ccgaccgcta cacggagaac cgcgctaccg gcgccttcat catcatcgac gagagcacca
     1201 acaacaccgt aggcgcgggc atgatcggct gatcgccgcc cgccctccgc agcgagcgaa
     1261 ccccggcccc cgcaagggcg ccggggttct tcattttggg gacgaagaaa cttttaccgc
     1321 atcacagaac atgacaatcg ggcattaaat tttctataat gaaattccat acgactattt
     1381 tcaggtcttt tcagcacctg caagagtcat ccccggatcg caccgaaaac cgctgcctgc
     1441 catcttcatc cgtcctcgag gaagtctccc gatgctcaag cgaatcaccc tcgtcaccgt
     1501 cgccaccttg gtcctcgccg gctgcgccgc cacccacgtc gccatcagca agcgcgacct
     1561 cgatgtgcag accaagatgt ccgacacggt cttcctcgac ccggtcggcc ctgacaagcg
     1621 caccatcttc ctgcagatcc gcaatacctc cgacaagccc gaccttgcgg tgaatgacga
     1681 gatcgccgcc gcgctgcaga ccaagggtta caccctgctg caggacccgg aggcggcgca
     1741 ctacgtcctg caggcgaaca tcctgcaggt cggaaaatcg gcacccaccg cgatcgaggc
     1801 ggcgatcgac caaggctacg gggtagccgc ctcgggcggc ctggtcctgg gggcgggagc
     1861 ggcatacgcc ggcggcgcca gcggccgtgg catcgccgcc gccggcctga tcggctcgct
     1921 ggccgaaggc atcgccggcg ccgcggtcaa ggacgtgtat ttttcgatga tcaccgacct
     1981 gcagatcaag gagcgcatcc gcggtgccgg caaggcaagg gtgagttcga accacagcct
     2041 gcgccagggc aacagcggtg gttcgcaggt gtcgtacgac gaagagtcgt cctacaagac
     2101 ttatcagacc cgcatcggct cgaccgcgaa caaggtcaac ctcgccttcg aggaggcgct
     2161 gcccgagttg aagaaaggcc tgatcaattc gatctccggg ctgttctgaa cgctcgcggg
     2221 cttcatctgc cccagcacgc cgccgggtgt tcgataatca cggcggcgcc ttgccaatcc
     2281 ggcgcccttc gaccgagcgc caccatcatg accgaccgca atccctccga caccgcgcgt
     2341 acccgcctcg acaagtggct gtgggcggca cgtttcttca agcaccgtac gggcgcgacc
     2401 gaggcggtcg atggaggaaa ggtcaagctc aacggaatgg cggccaagcc ggcacgcgaa
     2461 ctcaaggtcg gtgaccgcct cgacatcacg atcggcgagg atacgcgcac ggtgatcgtg
     2521 cgcgcgatcg cggacaagcg cggctcggcc gccgtggcgc aggcgctcta cgaggagacc
     2581 gcggaaagca tcgaggcgcg cgaacgcgcc cgtgaattgc gcaagttcgc cgccgcgccc
     2641 ggcgccgacc tgcacggccg gccgaccaag cgcgaccgcc gcaggatgga ccgcttcggg
     2701 ggatgctgag ctcgcgtccc caagcgcgag ccctcgcggt gcccgatccg taccggctcc
     2761 gctccgactc ccgtgcctca gtcgagtacc agcgccagcg cgatcggcag gaaaaccacc
     2821 gcagcgaggt tgccgatcag caccatcgag gccaccttgt cgggctcctg gtcgtaacgc
     2881 tcggcgaaca tgtagttcag caccgccggc ggcagggcgc cgaacacgag caacagcgcc
     2941 tgctcgcgcg gcggaagctc gagcatctgc agcagggccc aggccagcgc catgcccacc
     3001 accgggcgta gcaccgcacc gaagaggccg aagccgacgg cgctgatgcg cgaatcggcc
     3061 aggcgcacgc cgagcgcgaa caacatcagg gggatcgaga tgtcgccgac catctcgatc
     3121 gacatcagca gcggcggcca cacctcgatg ccgaggaagc cgaccgccag gcccgcgatc
     3181 gtcgccagga tggagggcga gcgccacacc gtggtcagct tggtatggtg gtccagcagc
     3241 caggcgccgt aggagaagtg gatcacgttc gacaccacga acatcaccac cgccggagcc
     3301 agcgccgact cgccgaaggc gagcaccgcc agcggcaggc cgaggttgcc gcagttgttg
     3361 aacatcatcg gcggcaccag cgtcttggtg gcgatcccgg ccgcacgcgc gaccagccag
     3421 ccggccacac cggagccgac gatgatcacc acggtcgcga acagcagcgg caggtattcg
     3481 ccgaggcgga agtccttgcc gaccagcgca ccgaacacca gcgcgggcac gaagacatcc
     3541 atgttcagct tgttggcgtg cgacaggtcc ggcttcatgc gccggccgac gaaatagccc
     3601 agcgcggtga tcgcgaacag cgggaacagg atggagacga ttcggatcag cattcagggc
     3661 aaaccgggag cggactgaag ggggtgcgac ggatgcggtc acggacaagc ccgtcgtcgc
     3721 gcggtccccg cggacgaagc gagcttgtcc gcgaacggac gcgagccggg atcaaagcac
     3781 gtaacgcgcc agatcctcgc gctgggcgag catgacgagc cggctgtcga cataggcggc
     3841 gtcgactacc acggtctgct ccgcactgct gcgcccggcc tcgaaggaaa cctcttcgag
     3901 cagcttctcc atgacggtgt acagccggcg cgcgccgatg ttctcggtct tctcgttcac
     3961 ctggtaggcg atctcggcca ggcggcggat gccgtcgtcg gcgaactcca gcttcacccc
     4021 gtcggtggcg agcagcgcct cgtactggcg cgtgaggcag gcgtcggtgc tggtgaggat
     4081 gcgggcgaag tcctccacag acagcgactc cagctcgacg cggatcggga aacgcccctg
     4141 cagctcgggg atcagatccg agggcttgga caggtggaag gcgccgctgg cgatgaacag
     4201 gatgtgatcg gtcttgatca tgccgtactt ggtggagatc gtcgtgccct cgaccagcgg
     4261 cagcaggtcg cgctgcacgc cctgacggga gacatctgcg ccctgcacgt cgctgcgcgc
     4321 ggcgatcttg tccacctcgt cgaggaacac gatgccgttc tgctcgaccg cgcgcacggc
     4381 ctcgagcttg acctcctcgt cgttgatcag gcgcgcggcc tcctcgtcgg cgagcagctt
     4441 catggcctcg cggatctgca gcttgcgctg cttcttcttg ccgccgccga ggttctggaa
     4501 catgccctgg atctgctggg tgagttcctc catacccggc ggggcgaaga tctcggcctg
     4561 catcgagggc gcggcgacct cgatctcgat ctccttgtcg tcgagttcac cctcgcgcag
     4621 cttcttgcgg aatttctgcc gggtggacga atcctgcgcc ggctcgggct cggcgttgaa
     4681 gccgaccggg cgcgccggcg gcagcagggc gtcgagcacg cggtcctcgg cggcatccag
     4741 cgcgcggtcg cgcaccgact tcatcgcacg ctcgcgcccg tccttgaccg cgatctccat
     4801 gaggtcgcgg atgatggtgt cgacgtcgcg gccgacatag ccgacctcgg tgaacttggt
     4861 cgcctcgatc ttgatgaagg gcgcgttggc caggcgcgcc aggcggcgcg cgatctcggt
     4921 cttgccgacg ccggtggggc cgatcatgag gatgttcttg ggggtgatct cgctgcgcag
     4981 cggctcttcc acctgagcgc gccgccagcg gttgcgcagc gcgatcgcca cggccttctt
     5041 ggccttgtcc tggccgacga tgtgcttgtc gagttcggag acgatctccg gcggggtcat
     5101 ctgggtcatg cggtcagcct caatccagca cttcgatggt gtggtggtga ttggtgtaga
     5161 tgcacaggtc gccggcgatg gaaagcgcct tggacacgat ttccctgggc tcgagctcgg
     5221 tattctcgag cagtgcgcgc gcggccgact gcgcgtaggc gccgccgctg ccgatggcca
     5281 cgatgccctg ctcgggctcg agcacgtcgc cgttgccggt gatcaccagc gaatgctcgc
     5341 gatcggccac cgcaagcatg gcctccagcc ggcgcagcat gcggtcggtg cgccagtcct
     5401 tggcgagctc gaccgcgctg cgcaacaggt tgccctggtg cttgtcgagc ttggcctcga
     5461 aacgctcgaa caaggtgaag gcgtcggcgg ttccaccggc gaaaccggcg aggatctgac
     5521 cgttgtagag cgtgcgcacc ttgcgcgcgg aggccttgat gacgatgttg ccaagggtga
     5581 cctggccgtc gccgccgagc gcgacgcggt tgccgcgccg caccgacagg atggtggtgc
     5641 cgcgatactg ttccatgaag gattctccgt ccccgcggaa atcggggctg gtgtgttgtt
     5701 caatgacgca gggtgacgtg agccaccttg tcgacgctca tgacccgcag caggccggcg
     5761 accatggcgt tcacgtcgcg aagacagacg agtttacctt gagcctgcaa cgtggcgaga
     5821 atattgaaga gcgtgccggc gcagacaaaa tcgagccggc gcaggcgccg gcaatcgatc
     5881 cggacctcgc ggcgctcgac cgcgaaggcg gcgaggcgcc ggagggtatc gttggcagcc
     5941 ccgcagagct cgccctcgaa cgcgaacgcc tccggatcgg tcggctgcgc ctcgtccgcc
     6001 tgcgccgcgc gcgccgtggg agcgtcgccg cgcccctccc aggacggcgg ggactcctcg
     6061 aaggtcaccg catagtcgac cgcgacatcc tcgaagcgcg cctggtcgcc ggcgtactgc
     6121 aacagctcga gcagcatcag ccaggtatcg cgcgcctgcg ccttcccggg ggatacggcc
     6181 tgcgacagga tgtcggcgag cgcgcgcaca ttgaccagcc tcacctgaag ccgctcggcc
     6241 gccagcccgg caagcaggcc gcgccagacg gcgcagcccg catcgtccac cccgcgcacc
     6301 ttggcgacgt cgatgcgcac cccgccgctg cggcgcgcga tcttggcgag ctgggcgaac
     6361 tggcgctcgg cggctgccga gagctgcccg ctcaggttga ccagcggcag ctcggtgccc
     6421 gccgccttgc ccgccacccg cgacagatcc tcccagggcg gcggcgagcg ctcgaagcgg
     6481 gtggcgtaat cgagcacgcg cgactcgaag cgctggcgct ccccgcgcag gcggtagaga
     6541 tcgagcaaca tcagccaggc accctcaccg ccctcgccac cttccgcgcc gacggcggcc
     6601 tcgagcacgg cgagcgcgtc gtcgtcgcta ccgttcgcat acagcaccgc agcctcctcg
     6661 aaggccgcgc cgatcccgct tccgcactcg cgcacctcga cctgcccggc cacgcgtgcg
     6721 agcgcgcgcc cgtggtcggt gccggtgaag tcgagcgcgg acaactcggt aggcggggga
     6781 cgctcgtcgc gcagggcgtc cgctcccggc ccggctcgcg cgtcgcgcgg tgcgccgggc
     6841 gcgggcgaaa cgggtttctt gccgaagaag gggagcgcca cgtccgtatt ccgatgtcgc
     6901 ggacgccccg tgcgagccgc cgcaaccaac gacattcaca gaaaagcgct tttgcaacaa
     6961 gcaataagcg cgagaaaaat ccatcgatca agaacgcgac agacgtgcat tcggtgccgc
     7021 ggctcggcta gaatgctgca atgcaaccga aaaccgtttc cgacgcgggc ggcgaatcgc
     7081 tccccgcgtc gtcgcgtttt tccctgtccg tccgagtcta ttacgaagac accgacgcag
     7141 ggggtgtcgt ctattacgca aactatctca aattctgcga gcgcacgcgc accgaatggc
     7201 tgcgtgcgct cggcgtcgcc cagcacgcct tgctcgacga gcagggcgtg ggtttcgtgg
     7261 tgcgctcggt gcaggccgac tacctcgctc cggcacgcct ggacgacgcg ctcgaggtga
     7321 gcgcccgcat cgccagccgg cgccgcgcga gtctcgtgtt cgaccacgag atccgccgcg
     7381 gcggcgaact tctcttcacc gcgcaggtcc tcgtggcttg cgtggacatg cggcgcatga
     7441 agcccgcagc cctccccgcc tccctgcacg cccttctcga aagcccagca tgaccgtcac
     7501 ccacgacctc tccatcctca gcctgatctc ccaggcgagc gtcctcgtcc agctcgtcat
     7561 ggcgctgctg gcaagcctgt cgctcgtctc ctggtactgg atcttccgca aggccttcca
     7621 gatccgccag gcgcgtgcac agaccgacga gttcgagcgc gacttctgga gcggcggcga
     7681 cctcaacacc ctctacgagt cggctggccg ccaccagtcc gaaggcatgg agcgcatctt
     7741 cgaggccggc taccgagagt tcaccaagct gcgcggcaag ggccacgacc acggcgccac
     7801 gctcgacggc gcccgccgcg ccatgcgcgc cacctaccag cgcgagatcg acgacctcga
     7861 ggcccacctc gccttcctcg cctcggtggg gtcggtgtcg ccttacatcg gcctgctcgg
     7921 caccgtgtgg gggatcatga actccttccg cggcctctcc agcgtcggcg ccgccaccct
     7981 cgcgcaggtc gcgcccggca tcgccgaagc cctggtcgcc acggcgatcg gcctgttcgc
     8041 ggcgatcccg gcggtggtgg cctacaaccg cttcgcgcac gacatcgacc gcctcggcat
     8101 ccgcttcgag agcttcatgg aagagttctc caacatcctg caacgcaacc tgcgctgacc
     8161 cggacggaat tccaacgatg cgccaacgcc gtctcatgaa ccagatcaac gtcgtgccct
     8221 acatcgacgt gatgctggtg ctgctggtga tcttcatggt caccgcgccg atgatccagc
     8281 agggcacgat caacgtgccc tccgccggcg ccggcacgcc gccgccgcag tccgaggcca
     8341 tcttcatcga ggtcaaggcc gacggggcct atgcgctgcg cgccagcaac agcgggagct
     8401 cgcgcccggt gcgccgcgac cagctgtcga ccgcgctcaa ggacgccctc gcgaagaacc
     8461 ccgagcagcc cttcctggtc gccgccgaca gcaagctcga ctaccagaag gtgatcgacg
     8521 tgctcgagac cgcgcgcgac gccggagtgc gcaagatcag cctggtcacc cagagcgcga
     8581 cttcgcagaa atgagggacc gccccgcacc gcaccgccgc gagccgcctg gcaagtggca
     8641 gtccctcggg ttgaccgtgg cggtccacct gggattgttc ctgttcctgt tcttcggcat
     8701 ccgctggcag agcccgccgc ccgcggcgct cgaggtcggg ctggcttcgg caccggagcg
     8761 cagcgcgcca gccgcagcgc gcccgcagcc ggcgccggag ccccggccgg agccgaaacc
     8821 cgaaccgaag cccgagccca aacccgaacc gaagcccgag cccaaaccgg aaccgaagcc
     8881 ggaacccaga cctgaaccga agccggaacc cagacctgaa ccgcccaagc cccaggccaa
     8941 gcccgagatc gtcgccaagg cgcccgagaa gaagccggag ccgcccaagc ccgagccacc
     9001 gaaacccgaa cccaaaccac aaccgaagcc ggagcccaag cctgaaccga agcccgagcc
     9061 caagccccaa cccaagccgg agccgaaacc cgagcccaag ccgcaggcca agcccgaacc
     9121 caagccccag cccaagccgg acaccaagcc gatcgacgac tacatggcgc agcgcctcgc
     9181 gcaggagacc cagcgcgccg agcaggcccg cctgtccagc ctgatggcgc aggaaggtgc
     9241 gcgcgcggcc gcggccggtc cgagccgcca gggtccgccc ggtggcgaca tcgacaaata
     9301 ccgcgcggcg atcgctgcca aggtgcgcgg caacctgttg cgcccgcccg gcctgtccgg
     9361 caacccggag gcggtgttcg aggtcgacca gctgccgagt ggcgaagtgc tcaatgtccg
     9421 cctcaagcgc tcgtccggcg tgcccgccct cgacgaggcc atcgagcgcg cgatccggcg
     9481 ctcgagcccg ctgccgctgc cggacaacaa gaaccagttc gagcgtaccc tcgaactgaa
     9541 attccgtccg ctggcggacg attgagtccg tcatgtctat aatccgcggc tggagaaaac
     9601 cccgcatgat caaaacactg cacgcattcc gcctcttcct cgccggcctg ctcgccagcc
     9661 tcgcgctggg tgcgcacgcc cagctctcga tcgagatcac cggcaccggc gcctcgcgct
     9721 ttccggtcat catcccgatc ttcgagaacg agggccgcct gccgcagagc gtcaccgacg
     9781 tcgtgcgcgc cgacctcgag cgcagcggtc tgttcaccct gatcgacacc ggcccgctgc
     9841 cgatgcccga atcggtggcg cccgacctcg ccgcggtgcg cagtcgcggc gccgacgcgg
     9901 tgctcaccgg cagcctgatc ccgatggccg acggccgcta cgacgtgcgc ttccggctct
     9961 tcgacgcgca gaagcagcag gaactcggcg ccatgtcgct gccgatgagt ccggcgcaga
    10021 accgcctggt cggccaccgc atcgccgact tcgtgtacga gaagctcacc ggcctgccgg
    10081 gttacttcgc cacccgcatc gcctacgtga tcaagtccgg tcccaacttc gagctccaga
    10141 tcgccgacgc cgacggcatg aacgccgcca ccgcgctgcg ctcgcgcgag ccgatcatct
    10201 cgccttcgtg gtcgcccgac ggccagcgcc tggcctatgt gtccttcgag cagaagaagc
    10261 cgatcgtcta cgtgcacaca ctcgccaccg gccagcgccg cgtggtcgcc aacttcaagg
    10321 gctcgaactc cgcgccgagc tggtcgcccg acggcagcca gctcgcggtc gtgctcacca
    10381 aggacggcct gtcgcagctc tacgcgatcg gtgccgacgg ctccggcgtg cgccgcctcg
    10441 cgcaatcctc cggcatcgac accgagccct tctggggcgc cgacggccac atctacttca
    10501 cctcggatcg cggcggcagt ccgcagatct accgtattcc tgccagtggc ggcagcgccc
    10561 agcgggtgag cttcgaaggc aactacaacg tgacgccgcg cctgtcgttc gacggcaagt
    10621 tgctcgcctt catcacccgc aatgccggcc gcttccaggt ggccgtgcag gacgtcgcca
    10681 cccggcagac caccatcctc accgattcgg cacgcgacga atcgcccagc ttcgccccca
    10741 acggccgcat gatcctctac gccaccgaca ccggtggccg tggcgtgctg tcggcggtgt
    10801 cgaccgacgg gcgcatccgc cagcggctca cggtgcaggc cgccgatgtc cgcgaacccg
    10861 cctggggtcc gcagatccgg taaccatgaa gcggcaacaa agccgcatac ccctctctgc
    10921 tgtcatcaac actcgtactg gagcatcacc catgaagaaa ctcgtcctcc ccgcgctgct
    10981 cgccctgacc ctggccgcct gctcgagcac cggccccgag aacggcgccg cctccgtcca
    11041 ggaccgcggc accggcgtcg ccaccgtgac ggccggcagc gccaagggca gcggcatcgc
    11101 ggcgctcacc gaccccaaca acatcctctc caagcgcagc gtcttcttcg acttcgacag
    11161 cttcgtgatc aagcaggaag cccgcccgct gatcgaagcc cacgcccgct tcctggtgca
    11221 gaacccgcag atgaacatgc tcgtccaggg caacaccgac gagcgcggca gccgcgaata
    11281 caacctcgcc ctcggccaga agcgcgccga cgtcgtcaag caggccctca tgctgctcgg
    11341 cgccaaggaa tcgcagatcg agtccgtgag cctgggtgaa gagaagccgc gctgcgaaga
    11401 ccgcaccgag aactgctacg ccgagaaccg ccgcggcgac atgctctaca cgggcgagtt
    11461 ctaagagagt cccgacatga aacgccttct gccgctcgcc gcagcgttcg tcctcatccc
    11521 gagcgccccg gcgcacgccc agctcttcgg gggtgacacc gaggcccgca accagatctt
    11581 ccagctccgg caggacgttc aggggcagat cgagaccacc agccgcggcc agctcgagct
    11641 cgccatgcag aacgaacaga tgcgcgccga gctggcgcgc ctgcgtggcc agatcgaact
    11701 gctgaccaac gaggtggaga cgctcaagca gcgccagaag gatttctacg tcgacctcga
    11761 cgcccgcctg cgccggtacg aatccggtgc ggcgggcgcc gcctccgccg ccccggcctc
    11821 cggggatgcg gcgccggcgg caccggctgc ggccgcagcg cccccgcagg cacctgcccc
    11881 cgcgccagcc cccgcatccg cgaacaccga atccgccgac tatcagggcg ccctcgcgct
    11941 gctcaagaac ggcaagcaca aggacgcgca gaccgcgttc gagcgcttca tcacccgcta
    12001 cccggcaggc aacttcaccg ccggcgccca cttctgggcc ggcaacgccg cactgcaggc
    12061 gcgcgacgtc gccagtgcga atcgccattt caagacggtg atggacaagt accccaagga
    12121 gaacgtcgcg cccgacgcca tgctcggcct ggccaacagt cagcaggcca tgaacgacgc
    12181 cgccggtgcc aagcgcaccc tgcaatcgct tgtcgagcgc tatccgcaga gcaacgctgc
    12241 tcaggtcgcc cgccagcgcc tgagcgcgcg ctgatccatg tccgctgtcg aaacggcgcc
    12301 ggcgacggcc gtgcgcctcc gtatcaccga gatcttcgcc tcgctgcagg gcgaatccac
    12361 ccgcgtcggc ctgcccaccg tgttcgtgcg cctgaccggc tgcccgctgc gctgcacctg
    12421 gtgcgacacc gcctacgcct tcagcggcgg cgagacacgc acgctcgacg acatcctcgc
    12481 cgaggtggcc agccacggtc tgcgccacgt gtgcgtgacc ggcggcgagc cgctcgcaca
    12541 gaagggctgc ctaccgctgc tcgccgcgct gtgcgatgcc ggccacgacg tgtcgctgga
    12601 aacgagcggc gcactcgaca tcgcgggggt cgatccccgc gtgtcgcgca tcgtggacct
    12661 caaggccccc ggctcgggcg agctcgcccg caaccgctgg gaaaacctcg cgctgctgtg
    12721 cgctcacgac gagctcaagt tcgtgctcgc cgacgccgcc gactacgcat gggccaagca
    12781 gcagatcgcc gagcaccgcc tcgccgaacg ctgcggcgtg ctgctgtcgc cggtcgccgg
    12841 cgcgctggac cctgccgagc tcgccggatg gatcgtgcgc gaccgcctgc cggtgcgctt
    12901 ccagctccag ctccacaaga tcctgtggaa cgacgcccgc ggccgctgag cgccgctgtt
    12961 ccggcccggc gacgccgcga gcagatccac cccgccccgc catccagaat gactccggaa
    13021 tgaccagcct ccacacccca ccccgcgccg tcgtgctgct ctccggcggc ctcgactccg
    13081 ccacctgcct cgcgatcgcg cgcgacatgg gcttcgagac ctacgcgctg tcggtcgcct
    13141 acggccagcg ccacgccgcc gagctcgccg cctcgaagcg cgtggcgcaa gcgctcggcg
    13201 cgcgcgagca ccgcaccgcc agcgtcgaac tcggccagtt cgggggctcg gcgctcaccg
    13261 accccggcat cgcggtgccg ctcgatcagg acaacaccgg catcccggtg acctacgtgc
    13321 cggcgcgcaa cacggtcatg ctgtcgatcg cactcgcctg ggccgaggtg ctcggcgcca
    13381 acgacatctt cgtcggcgtg aatgcggtcg attactcggg ctaccccgat tgccggccgg
    13441 tcttcatcga ggccttcgag cacatggcgc ggctggccac caaggtcggc gtcgagggcg
    13501 cgcagctgca catccacgcc ccgctgatcg agctctccaa ggccgacatc atccgccgcg
    13561 gcaccgccct cggcgtggac tacggcctca ccgtcacctg ctaccaagcc gacgacgacg
    13621 gccgcgcctg cggccgctgc gaagcctgcc gcctgcgcgc tgccggtttc aaagccgccg
    13681 gcgtgcccga tccgacgccc taccagccgc gttcggcctg accccctgcc gcgccatgga
    13741 ccacgccctc gtctccgcct ccaccatcgt ctggctcggc ttcgcgatcg gcgccgcgtt
    13801 cggcgcttcc agccagcgca ccggcttctg caccatgggc gcggtctccg acgcggtgaa
    13861 catcggcgac tggacgcgga tgcggatgtg ggcgctcgcg gtcgcgctcg ccaccctcgg
    13921 caccggcgcg ctgcacgccc tcgggctctt cgaccctgcc aagaccctct acaccggcgc
    13981 ccgcctgccc tggctgtcac acatcgtggg cggactcacc tttggcgtcg gcatgacgct
    14041 cgcatccggc tgcgccggca agaccctgat ccgcatcggc ggtggcaacc tcaagtcggt
    14101 cgtggtgttc gtcttcctcg ccatcggcgc ctcgatgacg ctgcgcgggc tcaccggcgt
    14161 gtggcgggtc gagttcctcg acccggtggc gctgcagctg ccccacggcc agaccctgcc
    14221 cgccttcctc gcggcggccg gcatggaggc aggcaaggcc gcgctcgccg ccagcgcgct
    14281 cgccggcggc ggcctgctcg cctgggcgct ggccagccgc gacgcgcgcc accccgaggt
    14341 gctgctcggc ggggccatcg tcggcctcgc ggtggtggcc ggctggtacg tcaccggcca
    14401 cctcggccac atcgccgagc accccgacac cctcgaggag gtcttcctcg ccaccaacag
    14461 cggccgcgcc gagtcgctca gcttcgtcgc cccgcaggcc ttcaccctcg agctgctgct
    14521 gctgtggagc gatgccaacc gccagatgag cttcggcatc gccagcgcgc tcggcgtgat
    14581 cgccggcgcg gcggcgatgg cgctggccac gggcagcttc cgcatcgaga gcttccgcga
    14641 ccccgccgac cttgtgcgtc acatcgccgg cggcctgctg atgggcttcg gcggcgtcac
    14701 tgcgctcggg tgcacgctcg gccagggcgt ctcgggcctg tcgaccctcg cgctcggcgc
    14761 cgcgatcacc accgccgcca tcatcgcggg agccgttgcg acgatgaagc tccagtactg
    14821 gctgccgacc cgcgcaggct gaagaggcaa accgccggcc gctctgcccc gacgagccac
    14881 cctgcggctc catcggggga ccgcgaaacc ggcaaggcgc ttgacgacgt ttccatcggc
    14941 cattatcatt cggcctcgct tttgggtcgt tagctcagct ggtagagcag cggactttta
    15001 atccgttggt cgcaggttcg aatcccgcac ggcctaccaa
//
LOCUS       DAOQBS010000071        15034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3027_length_15034_cov_16.669204, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000071 DAOQBS010000000
VERSION     DAOQBS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 15034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15034
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3027_length_15034_cov_16.669204"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1235)
                     /locus_tag="PKN25_09385"
     CDS             complement(210..1235)
                     /locus_tag="PKN25_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNV91152.1"
                     /translation="MNERQHAPAVTVSIRMVCQILDRLGEHRPLALECVERAGIASAL
                     LEHESARVTVEQFALFYRTLAVELDDETPGLFSRPLRPGTLKFLCLGMLDAANLRVAL
                     HRFCWFFRLVLDDLHFELGEDEGLSRIALVERVALQPHRPLILELMLMLVQGIASWMI
                     ERKLLFARVDLAYPAPPHAGEYINMFAGPACFDRPLTALYIEPAFMDAPIRQDKAALS
                     AFLRKAPMDWIHVSVSERLFTHRVRDLLEAGLGSPQSVEDVARMLHISARTLARRLDA
                     EGTHFQAVKDALRRDVAIARISRTDEPIGSIGADLGFDDPAAFNRAFKQWTGSPPGSY
                     RRAGAGR"
     gene            1453..2286
                     /locus_tag="PKN25_09390"
     CDS             1453..2286
                     /locus_tag="PKN25_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV91153.1"
                     /translation="MHPGKASPILEVEGLTLSFGGIKALTDVGFEVAPGSITTVIGPN
                     GAGKTSLFNTISGFYKPSVGRIRFQGADITATRPPERARLGLARSFQNIALFRGMTVL
                     DNIKLGRHAHLRTNVFDALVYFGRARHEEMKLREEIEERIIDFLEIDHIRHMPVSVLS
                     YGLQKRVEMARALAMRPKVLMLDEPVAGMNREETEDMARFILDVREEWGVTVLMVEHD
                     MGMVMDLSDHVVVLNFGQVIADGTPAEVQADPEVIKAYLGSGEVSELRQRLHQALAVG
                     A"
     gene            2288..3178
                     /locus_tag="PKN25_09395"
     CDS             2288..3178
                     /locus_tag="PKN25_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV91154.1"
                     /translation="MSLEFDWLFLAEIVLAGLGAGGLYALTGLAFVMIYKATRVVNLA
                     IGEMLMIGAYLCFGLATAMNLPMWLAILVAVIGSGGAGALLERVAIRPMLGESPIAVF
                     MVTVGLASILVGVVELIWTSEPRRLPEFMPATPVMIGEAFVPPKVFYGFLVAAVLIGV
                     ALAVFRFWRGGVALRATASDQQAAYSMGIDVPKVFSLSWTVAAMIAAIAGVIVGAVGG
                     ISSTMGVFGLSVLVVVIVGGLDSVLGALVAGLLIGVVESLAGGFLGGEYKLLATFLIL
                     IVVLLVRPYGLFGTHEIERL"
     gene            3178..4218
                     /locus_tag="PKN25_09400"
     CDS             3178..4218
                     /locus_tag="PKN25_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV91155.1"
                     /translation="MRIGTARSSYRHDEALFDSRTQHAWLAILAVALIGFPFLADEYW
                     LYLACLVAINIASTTGLNILTGYTGLVSLGQAAFMGIGAYTVAALEQKLGAPVLLNLL
                     AGGFVAMLGGLVVGLPSLRVKGLYLAIATIAASVVLHFVFANWHAVTGGHAGITLVPA
                     EILGLSFDTSFRLYWLFVPITILMVAGAANLFRTRIGRAFIAIRDRDISAEVLGIPLL
                     RYKLLSFGLSSFYAGVAGGMWAYFFRVVTPESFPLILSIFFLAAVIVGGMGTILGAIL
                     GAVFMTMVPEALKLVVGWLPLSADATLILSPVRTIVFGVLIVGFLVFEPLGLQEIWRR
                     IRRFFHLWPFRA"
     gene            4268..5437
                     /locus_tag="PKN25_09405"
     CDS             4268..5437
                     /locus_tag="PKN25_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV91156.1"
                     /translation="MRRTVLKSLLATAILSATAGLPVARAADKEIVIGGSIPMSGVFA
                     FAGIGIHAGIQDYVKMVNAAGGIAGHTLRYVPEDSAYKVDQSVAAFKKITSQNKVNFY
                     YGDSTAFSKTINPELDRAGSMIMSGASFATELNDPKKYPYQFVAGPDYTQQFGILLRY
                     IAQQKPGAKVAFVHSDTEFGRDPIETSRGVAKALGLDVAIEIMTPPGAVDVSTEIAKL
                     RRARPDFTIFHGYILGPIPEFIGQARQMGLDTQFMGTFWTMDNSTVMQMGELAEGFMG
                     VMPYNYYYDQSAPAPMLDKIRAMRPEYQNVPYIQGFLSAMLYAESARRTLAAGKEMSA
                     ANLKAALNSIENFDTGGLIGVPVTLRGNTIPVGRIYRADTKARRMVPASDWIVLE"
     gene            5441..6253
                     /locus_tag="PKN25_09410"
     CDS             5441..6253
                     /locus_tag="PKN25_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV91157.1"
                     /translation="MSAAESVLEVNNIEVIYNKAVQVLRGLSLAVPRGQIVALLGSNG
                     AGKSTTLKTVSGLLGLENGELTRGEIRFEGRPLADSKPHELVRAGLFHVMEGRRVFED
                     LSVEENLVAATYALTGRKGEKADKELVYEYFPRLYERRNGLAGYLSGGEQQMLAIGRA
                     LIARPRLILLDEPSLGLAPLLVENIFRIIARINAEQKVSMLLVEQNASVALAVAHYGY
                     IMENGKVVTDGPAERLAGDQDVREFYLGMGGHGEMKSFRDIKHYKRRKRWLS"
     gene            6268..8097
                     /locus_tag="PKN25_09415"
     CDS             6268..8097
                     /locus_tag="PKN25_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV91158.1"
                     /translation="MSNNLPTLTLPQMLREQARLQPGRPAIRQKDFGIWQAIGWDDYY
                     RRASHVGLGLRALGLPAGGHVGVLAENRIEWVLAQMGCGLVGAIAVGVYPTSPAPEVA
                     YVLNHADVEVVVCEDQEQTDKVIEALDQLPHLRAIVVMETKGLRNADERVRDRILSFA
                     DLERLGVEYEATHRHLVEETLAAQRLDDLALMIYTSGSTGKPKGAMIRYANIRGVAPG
                     IIDRLGLAADTVHLSYLPLCHVAEQMLTTFCPIYLGSTVAFGESIRTVQEDLREIAPT
                     MFLGVPRIWEKLHSAITIRMQESGPVQRWLFARALAACAPFAHKSAAQRTAGERLTHA
                     ICYGLILRALQNFIGLRKARVALTGAAPIPPAVVHFFRTLGVPLVEVYGLTESTGMIT
                     GQPLDAVAFGSVGTPILGVEHRIGARGELLVHGEMVFAGYYKNPEATADAIRDGWLHT
                     GDVVEERDGQLYIVDRLKDIMITAGGKNLTPSEIEKAMKSSPYIKECIVVADGRKFVS
                     ALIQIDYETVSRWAEGRQIPFTHFRSLVEHPEVRALIEAEIARGNAQLAQVSHIRRFH
                     LLNKELDHDDGEVTATMKVRRASVYKTYADVIEAMYRGGAAHA"
     gene            8090..8905
                     /locus_tag="PKN25_09420"
     CDS             8090..8905
                     /locus_tag="PKN25_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNV91159.1"
                     /translation="MPDPIHGDCVRLEREGGLAFITLDRPQARNALDLAMGLGLRDAV
                     RELAAAPPRVLVLRSSGAHFMVGGDVRRFDALLGESQAACTDEIGRLIDAVHEAVTGL
                     TRLPCPVLGLAGGSVAGFGLALAMACDLLVAADDARLVLAYGAIGATPDGGASWQLPR
                     RVGLQRALAIALLDPQLDAAQAREIGLVAEVVPAAQLAEAGLALARRLAAGPAAAQAG
                     IKRLLRAGLDTDLATALDAEKASFLALSATPDFAEGVRAFCERRPARFARPSG"
     gene            9024..10160
                     /locus_tag="PKN25_09425"
     CDS             9024..10160
                     /locus_tag="PKN25_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV91160.1"
                     /translation="MDFQLNPEQREIRDLARRFAEREIAPRAAQADLDKAFPTEVHQR
                     AQELGLLGINLPETVGGGGLGCLELVLVTEAFCRGCLGIGTALCVNALATEPIVIAGT
                     DAQRSRYLGAAARGALASFAMTEPGAGSDVAGIRTRAERVEGGYRLTGSKIWISNANL
                     AEFFVVFAKTAPDGGHRGMSAFIVPRASAGLTVGEPLGKLGQRCSPTCEVFLDQVFVP
                     EDHRLGEEGGGFALAMAVFDHSRPMVAAFGVGLIERCLDESLAYATQRSSMGKRLIDH
                     QAVAHKLAEMRMRLEAARLLTYQSAWLLDQGRPNTIEASIAKAFAADAAMWAATEAVQ
                     IFGGMGYSTEYPVEKLFRDAKVLQIYEGTSEIQRNIIARELQRA"
     gene            10177..11376
                     /locus_tag="PKN25_09430"
     CDS             10177..11376
                     /locus_tag="PKN25_09430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNV91161.1"
                     /translation="MNPSNPSNPNEALILGAVRTPFGRRGGALRETRPDELLATVIGG
                     VLARTGLPGDKVGDVLAGCVSQAGEQGANIARQALLIAGLPAEVPGVSMNRMCGSSQY
                     ATHAAAQAVAAGDLDFAIGCGVESMSRVPMFLDLTLGRGDFRGFDNLHPKILERFAIP
                     HQVESAERIAGQWKISRTEMDDYAIESHRRAEAARAAGVHSEILPVAGVDKEGGAITL
                     AHDEGIRAVIDVERMRAMQPVFRQPGEGGVTAANASQMSDGAAAVLVGRRAAAEALGI
                     RPRARFRARVALGSDPVLQLTGVIPAARRALEMAGLSIRDMDWIEVNEAFASVALCFV
                     REFAPDLDRLNPWGGAIAHGHPLGGSGAGLLAKMLAGLECRDGRFGLQVMCIGHGMAT
                     ATVIERL"
     gene            11396..12163
                     /locus_tag="PKN25_09435"
     CDS             11396..12163
                     /locus_tag="PKN25_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV91162.1"
                     /translation="MKIANSVFLVTGGASGLGAATARMVVEGGGKVVLADLNEEAGKA
                     LAAELGAAARFVRTDVTDEASTQAAVEAAVQGCGGLHGLVCCAGVAPGEKVVGKDGPH
                     GLDRFVRGVMINLVGTFNPIRLAAAAMSKAAPNEEGERGVIVTTASVAAFDGQIGQAT
                     YAASKAGVVGMTLPIARELARYGIRVMSIAPGIFETPMLRGLPQAVQDSLGEMVPFPS
                     RLGRSSEYAQLVRSICENPMLNGEVIRLDGAIRMAAK"
     gene            12174..13742
                     /locus_tag="PKN25_09440"
     CDS             12174..13742
                     /locus_tag="PKN25_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNV91163.1"
                     /translation="MNPVHTTMIPADVRRAGRNLIGDAFARSVRRNPEREALRFEGRS
                     WRYAQLDRAANRVANRLLGLGLAVGDRVAAYGRNSDAYVLLWLGCVKAGLIHVPINYA
                     LLDAELRYIVEQSGARALFCDADMATHVEALGSDLPCEWRGTLHGGHERDVLEWAQAG
                     GTDTAPAIALDEEAIAQLLYTSGTTAAPKGAMMSHRALLAEYTSTLLATDIRAEDCAL
                     AALPLYHSAQMHVFLMPQLLVGATTLLIQAPQPERCFELIERERVTSFFAPPTVWIAF
                     LRHPAFVPARLASLQKGYYGASIMPVPVLQELAARLPALQLYNCYGQSEIAPLATVLR
                     PEEHAERPASAGRPIFNVETRIVDSDLNDVPAGEMGEIVHRSPQLMSGYWDKPEETAA
                     SFRDGWFRSGDVGYLDEAGYLYITDRIKDIIKTGGVVVASREVEECLYTHPAVAEVAV
                     IGLPDERWIEAVTAVVALKQGAQATAAELIAHVHARLAAFKVPKRVFFVEDMPRNASG
                     KLLKRELRVRYAET"
     gene            complement(13777..14268)
                     /locus_tag="PKN25_09445"
     CDS             complement(13777..14268)
                     /locus_tag="PKN25_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNV91164.1"
                     /translation="MNITQHTDYALRLLMYLGTCTERRVTIKEVAERFDISRSHLMKV
                     VTGLVAKGFVDGARGKGGGLRLARAATQIGLGDVVRGMEPSFELVECFGSGSHCLLDP
                     ACRLKGALAGALQAFFAALDRLTLADLIGDDGQLHAVLGIPQADAQAPRPTIEIRPGT
                     RAD"
     gene            14388..14744
                     /locus_tag="PKN25_09450"
     CDS             14388..14744
                     /locus_tag="PKN25_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV91165.1"
                     /translation="MKRHPHLVRLSREHHAALRLGRHLLAGGAAAELRAEHVALVTHF
                     AEEERELAPLLETGGHGALAARLRAEHAHLRSLFATAADGVREAAAGQALIDHVRFEE
                     RELFPAIETLFEEVLP"
     gene            14741..>15034
                     /locus_tag="PKN25_09455"
     CDS             14741..>15034
                     /locus_tag="PKN25_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNV91166.1"
                     /translation="MTHVVTEACIRCKYTDCVSMCPVDAFREGPNFLVIDPEECIDCT
                     LCVAECPVDAIVPEDELSDEQREYLALNAALAKDWPRIVEAHAPLPDADAWAKV"
BASE COUNT         2290 a         5353 c         5092 g         2299 t
ORIGIN      
        1 gcgccgatga tagtgaggtc cgccctcgtg aaagtaggtc atcgtcagac taacccacaa
       61 tacacaacgc cccacgcaca catccgtgcc tggggcgtcg tgccgtctac gcagaacgca
      121 ggggttgagc ccccgcggat ccgtccccgg gtacgcgcgc cgcaaggcgc gaacgcggcc
      181 gccgtccgtt cggcgaccac gtcacaggct cagcggcccg cccccgccct gcggtagctg
      241 cctggcgggg atcccgtcca ttgcttgaag gcgcgattga aagccgcggg atcgtcgaag
      301 ccgagatcgg cgccgatgct gccgatcggc tcgtcggtgc gcgagatccg cgcgatggcg
      361 acgtcgcggc gcagcgcgtc cttgaccgcc tggaagtgcg ttccttccgc atcgagccgg
      421 cgggcgagcg tgcgcgccga gatgtgcagc atacgggcaa cgtcttccac ggactgcggg
      481 cttcccagcc cggcttcgag cagatcgcgc acgcggtgcg tgaacagccg ctcgctcacg
      541 gagacgtgga tccagtccat cggcgccttg cgcaggaagg ccgacagcgc ggccttgtcc
      601 tggcggatcg gggcgtccat gaaggccggc tcgatgtaga gcgcggtgag cgggcgatcg
      661 aagcaggccg ggccggcgaa catgttgatg tactcgcccg catgcggcgg cgccggatag
      721 gcgagatcca cgcgcgcgaa caggagcttg cgctcgatca tccacgaagc gatgccctgc
      781 accagcatca gcatcaattc gaggatcagg gggcggtggg gttggagcgc aacgcgctcg
      841 acgagcgcga tgcggctcag gccctcgtcc tcacccagct cgaagtgcag atcgtccagc
      901 accaggcgga aaaaccagca gaagcggtgc agcgcgacgc gcaggttggc ggcgtcgagc
      961 attccaaggc acaggaactt cagcgtgcca gggcgcagcg ggcgtgagaa cagccctggc
     1021 gtctcgtcgt cgagttcgac cgcgagggtg cggtagaaga gcgcgaactg ctcgaccgtc
     1081 acacgggccg attcgtgttc cagcagcgcg gaagcgatcc cggcgcgctc gacgcattcc
     1141 agggccagcg gcctgtgctc acccagccga tccaggatct ggcagaccat gcggatggag
     1201 acggtgaccg ccggtgcgtg ctgacgctcg ttcatgtgca agagattggt ggcagaaaat
     1261 gtcaatcgat cggccggttc tgtcatccac aaggtcggcc atactgccta gtatggcgtg
     1321 caaatagctg cagcgaaaag ttttatgtct gcgtcggcgg ttcgaggcct ctcgtgtggt
     1381 cgcgctcgct ggcaattaaa gcacagcacg cacaggtcac ccgcaaagag gtggccggca
     1441 ggaggagaca aggtgcatcc aggcaaggcc agtccgatcc tcgaggtcga ggggctcaca
     1501 ttgtcgtttg gcggcatcaa ggcgctgacc gacgtgggct tcgaagtcgc tccggggtcc
     1561 atcaccaccg tcatcggtcc gaacggcgcg ggtaagacct cgctgttcaa cacgatctcc
     1621 ggtttctaca agccctcggt ggggcgcatc cgcttccagg gcgcggacat caccgccacc
     1681 cgcccgcccg agcgcgcccg gctgggcctg gcgcgcagct tccagaacat cgcgctgttc
     1741 cgcggcatga ccgtgctcga caacatcaag ctcggtcgtc acgcccactt gcgcaccaac
     1801 gtcttcgatg cgctggtcta tttcggccgc gcccggcacg aagagatgaa gctgcgcgag
     1861 gagatcgagg aacgcatcat cgacttcctc gagatcgacc acatccgcca catgccggtg
     1921 tcggtcctct cctacgggct gcagaagcgg gtggagatgg cgcgcgcgct ggcgatgcga
     1981 cccaaggtgc tgatgctcga cgagcccgtg gcgggcatga accgcgagga gaccgaggac
     2041 atggcgcgct tcatcctcga cgtgcgcgag gagtggggcg tgacggtgct gatggtcgag
     2101 cacgacatgg gcatggtcat ggacctctcc gaccacgtcg tggtgctcaa cttcggccag
     2161 gtcatcgccg acggcacgcc ggccgaggtg caggccgacc ccgaggtgat caaggcctac
     2221 ctgggctcgg gcgaggtctc cgagctgcgt cagcgcctcc accaagcgct tgccgtgggg
     2281 gcctgagatg agcctggaat tcgactggct gttcctcgcc gagatcgtgc tcgccggcct
     2341 cggcgccggc ggcctgtacg cgctgaccgg cctcgccttc gtgatgatct acaaggccac
     2401 gcgggtggtg aacctggcca tcggcgagat gctgatgatc ggcgcctacc tgtgcttcgg
     2461 cctggccacc gccatgaacc tgccgatgtg gctggcgatc ctggtcgcag tgatcggcag
     2521 cggcggggcc ggcgcactgc tcgagcgcgt ggcgatccgc cccatgctgg gcgagtcgcc
     2581 gatcgcggtc ttcatggtca ccgtgggcct ggcctccatc ctggtcggcg tcgtcgagct
     2641 gatctggacc tcggagccgc gccgcctgcc ggagttcatg cccgcgacgc cggtgatgat
     2701 cggcgaggcc ttcgtgccgc ccaaggtgtt ctacggcttc ctcgtcgcgg ccgtgctgat
     2761 cggcgtggcg ctggcggtgt tccgcttctg gcgcggcggc gtggcgctgc gggcgacggc
     2821 ctcggaccag caggcggcct actcgatggg catcgacgtg cccaaggtgt tctcgctgtc
     2881 ctggacggtg gcggcgatga tcgcggcgat cgccggcgtc atcgtcggcg cggtgggcgg
     2941 catctcctcg acgatggggg tgttcggcct gtcggtgctg gtggtggtga tcgtcggcgg
     3001 cctcgacagc gtgctcggcg cgctggtcgc cggcctcctc atcggtgtcg tcgagtcgct
     3061 cgcgggcggc ttcctcggcg gtgaatacaa gctgctggcc accttcctga tcctgatcgt
     3121 cgtgctgctg gtccgtccct acggactgtt cggcacccac gaaatcgaga ggctgtaatg
     3181 cgcatcggaa ccgcacgctc cagctaccgc cacgacgagg cgctgttcga cagccgcacc
     3241 cagcacgcct ggctggcgat cctcgccgtc gcgctgatcg gcttcccctt cctcgccgac
     3301 gagtactggc tctacctcgc ctgcctggtg gcgatcaaca tcgccagcac caccgggctc
     3361 aacatcctca ccggctacac cgggctggtg agcctgggcc aggccgcctt catgggcatc
     3421 ggcgcctaca ccgtcgccgc gctggagcag aagctgggcg cgccggtgct cctcaacctg
     3481 ctcgccggcg gcttcgtcgc catgctcggc gggctggtgg tgggcctgcc ctcgctgcgg
     3541 gtcaagggcc tgtacctggc gatcgcgacc atcgccgcct cggtggtgct gcacttcgtg
     3601 ttcgccaact ggcatgcggt caccggcggc catgcgggca tcaccctggt gccggcggag
     3661 atcctcggcc tcagcttcga cacctccttc cgcctgtact ggctgttcgt gccgatcacc
     3721 atcctgatgg tggccggcgc ggccaacctc ttccgcaccc gcatcggccg cgccttcatc
     3781 gccatccgcg accgcgacat ctccgccgag gtgctgggca ttccgctgct gcgctacaag
     3841 ctgctgtcct tcggcctgtc gtccttctac gccggggtgg cgggcgggat gtgggcctac
     3901 ttcttccgcg tggtcacgcc ggagagcttc ccgctgatcc tgtcgatctt cttcctcgcc
     3961 gcggtcatcg tcggcggcat gggcaccatc ctcggcgcca tcctcggcgc ggtcttcatg
     4021 accatggtgc ccgaggcgct gaagctcgtc gtcggctggc tgccgctgtc ggccgacgcc
     4081 acgctgatcc tgtcgccggt gcgcaccatc gtgttcggcg tgctgatcgt cggcttcctg
     4141 gtgttcgagc cgctcggcct gcaggagatc tggcgccgca tccgccgctt cttccacctc
     4201 tggccgttcc gcgcctgagc tttcctcgcc gcaccgcttc accccatatc aagagaagga
     4261 gacaagcatg agaagaaccg tactcaagag cctgctggcc accgcgatcc tctccgccac
     4321 cgccggcctg cccgtagccc gggcggccga caaggagatc gtcatcggcg gctccatccc
     4381 gatgagcggc gtgttcgcct tcgccggcat cggcatccac gccggcatcc aggattacgt
     4441 gaagatggtc aacgccgctg gcggcatcgc aggccacacg ctgcgctacg tgcccgagga
     4501 cagcgcctac aaggtcgacc agtcggtggc ggcgttcaag aagatcacca gccagaacaa
     4561 ggtgaacttc tactacggcg actccaccgc cttcagcaag accatcaacc ccgagctcga
     4621 ccgcgccggc tcgatgatca tgtcgggcgc ctccttcgcc accgagctca acgacccgaa
     4681 gaagtaccct taccagttcg tcgccggccc ggactacacc cagcagttcg gcatcctgct
     4741 gcgctacatc gcccagcaga agcccggcgc caaggtggcc ttcgtccatt ccgacaccga
     4801 gttcggccgc gacccgatcg agaccagccg cggcgtggcc aaggcactcg ggctcgacgt
     4861 cgccatcgag atcatgaccc cgccgggcgc ggtcgatgtg tccaccgaga tcgccaagct
     4921 gcgccgcgcg cggcccgact tcaccatctt ccacggctac atcctcggcc ccatccccga
     4981 attcatcggc caggcgcgcc agatgggcct ggacacccag ttcatgggca ccttctggac
     5041 catggacaac tcgaccgtga tgcagatggg cgagctcgcc gaaggcttca tgggcgtgat
     5101 gccctacaac tactactacg accagagcgc accggcgccg atgctcgaca agatccgcgc
     5161 gatgcgtccc gagtaccaga acgtgcccta catccagggc ttcctgtcgg ccatgctgta
     5221 cgccgagtcg gcccgccgca cgctggccgc aggcaaggag atgagcgcgg ccaacctcaa
     5281 ggcggcgctc aactcgatcg agaatttcga caccggcggc ctgatcggcg tgcccgtcac
     5341 cctccgcggc aacaccatcc cggtcggccg catctaccgc gccgacacca aggccaggcg
     5401 catggtgccg gcctccgact ggatcgtgct ggagtaagcg atgagtgcgg ccgaatccgt
     5461 cctcgaggtg aacaacatcg aggtgatcta caacaaggcg gtgcaggtgc tgcgcggcct
     5521 ctcgctggcg gtgccgcgcg ggcagatcgt cgccctgctc ggctccaacg gcgccggcaa
     5581 gagcaccacg ctcaagaccg tctccggcct cctcgggctg gagaacggcg agctgacgcg
     5641 cggcgagatc cgcttcgagg gcaggccgct ggcggattcc aagccgcacg agctggtgcg
     5701 cgcggggctc ttccacgtga tggaggggcg ccgcgtgttc gaggacctca gcgtcgagga
     5761 gaacctcgtc gccgccacct acgcactcac cgggcgcaag ggcgagaagg ccgacaagga
     5821 gctcgtctat gaatacttcc cgcgcctgta cgagcgccgc aacggcctcg ccggctatct
     5881 gtcgggcggc gagcagcaga tgctggcgat cggccgcgcg ctgatcgccc gccccaggct
     5941 gatcctgctc gacgagccct cgctgggcct cgcgccgctg ctggtggaga acatcttccg
     6001 catcatcgcc cgcatcaacg ccgagcagaa ggtgtcgatg ctgctggtgg agcagaacgc
     6061 ctcggtggcg ctggcggtgg cgcactacgg ctacatcatg gagaacggca aggtcgtcac
     6121 cgacggcccg gccgagcgtc tcgccggcga ccaggacgtg cgcgagttct acctcggcat
     6181 gggtgggcac ggcgagatga agagcttccg cgacatcaag cactacaagc gccgcaagcg
     6241 ctggctgtcg tgatcgagag ggtggccatg agcaacaacc tacccacgct gaccctgccg
     6301 cagatgctgc gcgagcaggc gaggctgcaa cccgggcggc ccgccatccg ccagaaggac
     6361 ttcggcatct ggcaggccat cggctgggac gactattacc gccgcgcgag ccatgtcggc
     6421 ctcggcctgc gcgcgctcgg cctgcccgcc ggcggccatg tcggcgtgct cgccgagaac
     6481 cgcatcgaat gggtgctggc gcagatgggc tgcggcctcg tcggcgccat cgcggtcggc
     6541 gtctatccga ccagcccggc acccgaggtg gcctacgtgc tcaatcacgc cgacgtcgag
     6601 gtcgtggtgt gcgaggacca ggagcagacc gacaaggtga tcgaggcgct cgaccagctg
     6661 ccgcacctgc gcgccatcgt ggtgatggag accaagggcc tgcgcaacgc cgacgagcgc
     6721 gtgcgcgacc gcatcctgtc cttcgccgac ctggagcggc tcggcgtcga atacgaggcc
     6781 acccaccgcc acctggtcga ggagaccctg gccgcgcagc ggctcgacga cctcgcgctg
     6841 atgatctaca cctcgggctc caccggcaag cccaagggcg cgatgatccg ctacgccaac
     6901 atccgcggcg tcgcgcccgg catcatcgac cgcctcgggc tcgcggccga caccgtgcac
     6961 ctgtcctacc tgcccctttg ccacgtcgcc gagcagatgc tgaccacctt ctgcccgatc
     7021 tacctcggct ccaccgtcgc cttcggcgag tcgatccgca ccgtgcagga ggatctgcgc
     7081 gagatcgcgc ccacgatgtt cctcggcgtg ccgcgcatct gggagaagct gcactcggcg
     7141 atcacgatcc ggatgcagga gagcgggccc gtgcagcgct ggctgttcgc acgcgcgctg
     7201 gcggcctgcg cgcccttcgc ccacaagtcg gcggcgcagc gcacggcggg cgagcgcctg
     7261 acccacgcca tctgctacgg cctcatcctg cgcgcgctgc agaacttcat cggcctgcgc
     7321 aaggcgcgcg tggcgctgac cggcgcggcg ccgatcccgc ccgcggtggt gcacttcttc
     7381 cgtaccctcg gggtgccgct ggtcgaggtc tacggcctca ccgaatcgac cggcatgatc
     7441 accggccagc cgctcgacgc ggtggccttc ggttcggtcg gcacgcccat cctcggcgtc
     7501 gagcaccgca tcggcgcgcg tggcgagctg ctggtccacg gcgagatggt cttcgccggc
     7561 tactacaaga acccggaggc gaccgccgac gccatccgcg acggctggct gcacaccggc
     7621 gacgtggtcg aggagcgcga cggccagctc tacatcgtcg atcgcctcaa ggacatcatg
     7681 atcaccgccg gcggcaagaa cctgacgccg tccgagatcg agaaggcgat gaagtccagc
     7741 ccctacatca aggagtgcat cgtcgttgcc gacgggcgca agttcgtctc ggcgctgatc
     7801 cagatcgact acgagacggt gtcgagatgg gccgagggcc gccagatccc cttcacccac
     7861 ttccgctcgc tggtcgagca ccccgaggtg cgcgcgctga tcgaggccga gatcgcgcgc
     7921 ggcaacgccc agcttgccca ggtctcgcac atccgccgct tccacctgct gaacaaggag
     7981 ctcgaccacg acgacggcga ggtcacggcg accatgaagg tgcgtcgcgc gagcgtctac
     8041 aagacctatg ccgacgtgat cgaagccatg taccggggag gggccgccca tgcctgaccc
     8101 gatccacggc gactgcgtgc gactcgagcg tgaaggcggg ctcgccttca tcaccctcga
     8161 ccgcccgcag gcgcgcaacg ccctcgacct ggcgatgggg ctggggctgc gcgacgccgt
     8221 gcgcgaactt gccgccgcgc cgccgcgcgt gctcgtgctg cgcagcagcg gcgcgcactt
     8281 catggtcggc ggcgacgtgc gccgcttcga cgccctgctc ggcgagtcgc aggccgcctg
     8341 taccgacgag atcggccgcc tcatcgatgc ggtgcacgag gccgtcaccg gcctcacccg
     8401 gctgccttgc ccggtgctgg gtctggcggg cggctcggtg gcgggcttcg gcctggcgct
     8461 ggccatggct tgcgacctcc tggtcgcggc cgacgacgcg cgcctcgtgc tcgcctacgg
     8521 tgccatcggc gccacgcccg acggcggcgc gagctggcag ctgccgcgcc gcgtcggcct
     8581 gcagcgggcg ctcgcgatcg cgctgctcga cccgcagctc gatgcggcgc aggcgaggga
     8641 gatcggcctg gtggccgagg tggtgccggc agcgcagctg gccgaggccg ggctggcgct
     8701 ggcccgacgc ctggccgcgg gaccggccgc cgcccaggcc ggcatcaagc gcctgctgcg
     8761 cgcgggcctc gacaccgacc tggcgacggc gctcgacgcc gagaaggcga gcttcctcgc
     8821 cctctccgcg acgccggact tcgccgaggg cgtgcgcgcc ttctgcgagc ggcggccggc
     8881 gcgcttcgcg cgaccctccg gctaggctcg cccgcgacgc gctgcccgag cgccttccac
     8941 ccgcaatccc cgtcggcgtg cccaggcatg cccgcccctt tattgcccgt cgtctgcgca
     9001 cggcggcttc gcatggagac accatggact tccagttgaa cccggagcag cgcgagatcc
     9061 gcgacctggc gcgccgcttc gccgagcgcg agatcgcccc ccgcgccgcg caggccgatc
     9121 tcgacaaggc tttcccgacc gaggtgcacc agcgcgcgca ggagctcggc ctgctcggca
     9181 tcaacctgcc cgagacggtc ggcggcggcg gcctcggctg cctggagctg gtgctggtca
     9241 ccgaggcctt ctgccgcggc tgcctgggca tcggcaccgc gctgtgcgtg aacgcgctcg
     9301 ccaccgagcc gatcgtcatc gccggaaccg atgcgcagcg cagccgctac ctcggcgccg
     9361 cggcccgcgg tgcgctcgcc agcttcgcga tgaccgagcc cggcgccggc tccgacgtgg
     9421 ccggcatccg cacccgcgcc gagcgcgtcg agggcggcta tcgcctcacc ggcagcaaga
     9481 tctggatctc caacgccaac ctcgccgagt tcttcgtcgt cttcgccaag accgcgccgg
     9541 acggcggcca ccgcggcatg agcgccttca tcgtgccgcg cgccagcgcc ggcctgacgg
     9601 tgggcgagcc cttgggcaag ctcggccagc gctgttcgcc cacctgcgag gtcttcctcg
     9661 accaggtctt cgtgcccgag gaccaccgcc tcggcgagga gggcggcggc ttcgcgctgg
     9721 cgatggcggt gttcgaccac tcgcgcccga tggtggccgc cttcggcgtc ggcctcatcg
     9781 agcgctgcct cgacgagtcg ctcgcctacg ccacccagcg cagcagcatg ggcaagcgcc
     9841 tgatcgacca ccaggccgtg gcccacaagc tcgccgagat gcgcatgagg ctcgaagccg
     9901 cgcgcctgct cacctaccag tcggcctggc tgctcgacca gggtcggccg aacaccatcg
     9961 aagcctcgat cgccaaggcc ttcgccgcgg acgcggcgat gtgggcggcg accgaggcgg
    10021 tgcagatctt cggcggcatg ggctactcga ccgagtaccc ggtggagaag ctcttccgcg
    10081 acgccaaggt gctgcagatc tacgaaggca ccagcgagat ccagcgcaac atcatcgccc
    10141 gcgaactgca gcgggcctga acccgaggac ccggaaatga acccaagcaa tccatcgaac
    10201 ccgaacgagg cgctgatcct gggcgccgtg cgcaccccct tcggccgccg cggcggcgcc
    10261 ctgcgcgaga cccgcccgga cgagctcctc gccaccgtca tcggcggcgt gctggcgcgc
    10321 accggcctgc ctggcgacaa ggtcggcgac gtgctcgccg gctgcgtcag ccaggccgga
    10381 gagcagggcg ccaacatcgc ccgccaggcg ctgctgatcg ccggcctgcc cgccgaggtg
    10441 ccgggcgtgt cgatgaaccg catgtgcggc tccagccagt acgccaccca cgccgcggcg
    10501 caggcggtgg ccgcgggcga cctcgacttc gccatcggct gcggcgtcga gagcatgagc
    10561 cgggtgccga tgttcctcga cctgaccctg ggccggggcg acttccgcgg cttcgacaac
    10621 ttgcacccga agatcctcga acgcttcgcc atcccgcacc aggtcgagag cgccgagcgc
    10681 atcgccgggc agtggaagat ctcgcgcacc gaaatggacg actacgcgat cgagagccac
    10741 cgccgcgccg aggccgcgcg tgcggccggc gtgcacagcg agatcctgcc ggtggcgggg
    10801 gtggacaagg agggcggggc gatcacgctc gcgcacgacg agggcatccg cgcggtcatc
    10861 gacgtcgagc gcatgcgcgc catgcagccg gtgttccgcc agcccggcga gggcggcgtg
    10921 accgcggcca acgccagcca gatgtcggac ggtgccgccg cggtgctggt gggccgccgc
    10981 gccgccgccg aggcgctcgg catccggccg cgcgcccgct tccgcgcccg cgtcgcgctc
    11041 ggctccgacc cggtgctgca gctcaccggg gtgatcccgg cggcgcggcg tgcgctcgag
    11101 atggccgggc tgtcgatccg cgacatggac tggatcgagg tcaacgaggc cttcgccagc
    11161 gtggcgctgt gcttcgtgcg cgagttcgcg cccgacctgg atcgcctcaa cccctggggt
    11221 ggggcgatcg cccacggcca cccgctgggc ggcagcggcg ccggcctgct ggccaagatg
    11281 ctcgccggcc tcgaatgccg cgacggccgc ttcggcctgc aggtcatgtg catcggccac
    11341 ggcatggcca ccgccaccgt catcgaacga ctctgagaca caaggagcac acgcaatgaa
    11401 gatcgcaaac tcggtattcc tggtgaccgg cggcgcctcc gggctgggcg cggccaccgc
    11461 gcgcatggtg gtcgaaggcg gcggcaaggt ggtgctcgcc gacctcaacg aagaggccgg
    11521 caaggcgttg gcggccgaac tcggcgcggc ggcccgtttc gtgcgcaccg acgtcaccga
    11581 cgaggcctcg acgcaggcgg cggtcgaggc cgcggtgcag ggctgcgggg gcctgcacgg
    11641 cctggtgtgc tgcgcgggcg tggcgccggg cgagaaggtg gtgggcaagg acggcccgca
    11701 tggtctcgac cgcttcgttc gcggcgtgat gatcaacctt gtcggcacct tcaacccgat
    11761 ccgcctcgcc gccgccgcga tgagcaaggc cgcgcccaac gaggagggcg agcgtggcgt
    11821 gatcgtcacc accgcctcgg tcgccgcctt cgacggccag atcggccagg cgacgtatgc
    11881 ggcgtccaag gccggcgtgg tcggcatgac gctgccgatc gcgcgcgagc tggcacgcta
    11941 cggcatccgc gtgatgagca tcgcccccgg catcttcgag acgccgatgc tgcgcgggct
    12001 gccgcaggcg gtgcaggatt cgctcggcga gatggtgcct ttcccctcgc gcctcggccg
    12061 ctccagcgaa tatgcgcagc tggtgcgctc gatctgcgag aacccgatgt tgaacggcga
    12121 ggtcatccgt ctcgatggcg cgatccgcat ggcagccaag tgaggagccc ggcatgaacc
    12181 ccgtacacac gaccatgatt cccgccgacg tccgtcgtgc cgggcgcaac ctgatcggcg
    12241 acgccttcgc gcgctcggtg cgccgcaacc ccgagcgcga ggcgctgcgc ttcgaggggc
    12301 gcagctggcg ctacgcccag ctcgaccgtg ccgccaaccg cgtcgccaac cgcctgctcg
    12361 gcctcggcct ggcggtcggc gatcgcgtcg ccgcctatgg gcgcaattcc gacgcctacg
    12421 tgctgctgtg gctgggctgc gtgaaggcgg ggctgatcca cgtcccgatc aactacgcgc
    12481 tgctcgacgc cgagctgcgc tacatcgtcg agcagtcggg cgcgcgtgcg ttgttctgcg
    12541 acgccgacat ggcgacccac gtcgaggccc tcggcagcga cctgccctgc gaatggcgcg
    12601 gcacgctgca cggcggacac gagcgcgacg tcctcgagtg ggcgcaggcc ggcggcacgg
    12661 acacggcccc ggcgatcgcg ctcgacgagg aggccatcgc gcagctgctg tacacctcgg
    12721 gcaccaccgc cgcgcccaag ggcgcgatga tgagccatcg cgcgctgctc gccgaataca
    12781 ccagcacgct gctcgccacc gacatccgcg ccgaggactg cgcgctcgcc gcgctgccgc
    12841 tgtaccactc ggcgcagatg cacgtcttcc tgatgccgca gctgctggtg ggcgcgacca
    12901 ccctgctgat ccaggcgccg cagcccgagc gctgcttcga gctgatcgag cgcgagcgcg
    12961 tgacgtcctt cttcgccccg cccacggtgt ggatcgcctt cctgcgtcat ccggccttcg
    13021 tgccggcgcg gctggcctcg ctgcagaagg gctactacgg cgcctcgatc atgccggtgc
    13081 cggtgctgca ggagctggcc gcacgcctgc cggcgctgca gctctacaac tgctatggcc
    13141 agagcgagat cgccccgctc gccaccgtgc tgcgtccgga ggaacacgcc gagcgccccg
    13201 cgtccgccgg gcggccgatc ttcaacgtcg aaacccgcat cgtcgacagc gacctcaacg
    13261 acgtgcctgc gggcgagatg ggcgagatcg tgcaccgctc gccgcagctc atgagcggct
    13321 actgggacaa gccggaggag accgcggcca gcttccgcga cggctggttc cgttcggggg
    13381 acgtcggcta cctcgacgag gccggctacc tctacatcac cgaccgcatc aaggacatca
    13441 tcaagaccgg cggcgtggtg gtggcgagcc gcgaggtgga ggagtgcctg tacacccacc
    13501 ccgcggtggc cgaggtcgcg gtgatcggcc tgcccgacga gcgctggatc gaggcggtga
    13561 cggcggtggt ggcgctcaag cagggcgcgc aggccacggc cgcagagctg atcgcccacg
    13621 tgcatgcccg gctggcggcc ttcaaggtgc ccaagcgggt gttcttcgtc gaggacatgc
    13681 cgcgcaacgc ctccggcaag ctgctcaagc gcgagctgcg cgtgcgctac gcggaaacct
    13741 gagcgccccg ggtggcggta cccgtgccga ctcgcctcag tctgcccggg tgcccggcct
    13801 gatctcgatc gtcggccggg gggcttgcgc gtccgcttgc gggatgccga gcacggcgtg
    13861 cagctgcccg tcgtcgccga tcaggtcggc gagcgtgagg cggtcgaggg cggcaaagaa
    13921 ggcctgcagg gcgccggcca gtgcgccctt gagccggcag gccggatcca gcaggcagtg
    13981 cgatcccgat ccgaagcact cgaccagctc gaagctgggc tccatcccgc ggaccacgtc
    14041 gccgagaccg atctgcgtcg ccgcacgcgc cagccggagt ccgccgccct tgccgcgcgc
    14101 gccgtcgaca aaacccttcg ccaccagccc ggtgaccacc ttcatcaggt ggctgcgcga
    14161 gatgtcgaag cgttccgcca cttccttgat ggtcacgcgt cgctcggtgc aggtgccgag
    14221 gtacatgagc aggcgaagcg cgtaatcggt gtgctgggtg atgttcatcg cgaaggtgcg
    14281 ccgggcgtat ggcgggctgc atggtagcag caatagattc atttctgttg actcttttaa
    14341 agatgcattt aatatgtatc ttaaaggcct cgaccgaagg agctgccatg aaacgccacc
    14401 cccatctcgt ccggctgtcg cgcgaacacc atgccgcgct gcggctcggc aggcatctgc
    14461 tcgccggcgg cgcggcggcc gagctgcgcg cggaacacgt cgcgctcgtc acccactttg
    14521 ccgaggaaga gcgcgagctg gcccccttgc tcgaaacggg cggtcacggc gcactcgccg
    14581 cgcgcctgcg cgccgagcat gcccacctga ggtccctgtt cgccaccgca gcggacggcg
    14641 tgcgcgaggc cgcggccggg caggcgctga tcgaccatgt gcgcttcgag gagcgcgaac
    14701 tgtttccggc gatcgaaacc ctgttcgagg aagtgctgcc atgacccatg tcgtcaccga
    14761 agcctgcatc cgctgcaaat acacggattg cgtgagcatg tgcccggtcg acgcctttcg
    14821 cgaggggccg aacttcctcg tgatcgaccc cgaggagtgc atcgactgca ccctgtgcgt
    14881 cgccgagtgt ccggtcgatg cgatcgtgcc ggaagatgag ttgagcgacg agcagcgcga
    14941 atacctcgcc ctcaacgcgg cgctggcgaa ggactggccg cgcatcgtcg aggcgcatgc
    15001 gccgctgccc gatgccgatg catgggcgaa ggtg
//
LOCUS       DAOQBS010000072        14992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3045_length_14992_cov_13.518444, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000072 DAOQBS010000000
VERSION     DAOQBS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 14992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14992
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3045_length_14992_cov_13.518444"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /gene="guaA"
                     /locus_tag="PKN25_09460"
     CDS             complement(<1..1562)
                     /gene="guaA"
                     /locus_tag="PKN25_09460"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNV91167.1"
                     /translation="MAHQKILILDFGSQVSQLIARRVREQQVYCELHPFDVSDEFVRN
                     FGAQGVILSGGPNSVYEAEDWRAPQAVFELGVPVLGICYGMQTMASQLGGKVESSAKR
                     EFGYAEMRARGHSKLFTGIEDRSNAEGHGLLDVWMSHGDKVTALPPGFKIIGSNESTP
                     IAAMADEARGFYGVQFHPEVTHTIKGKEMIGRFVHDICGCGHDWNMPDYIEEAVQKIR
                     EQVGDEEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKLVMEMFAGR
                     LHAKVVHVDATEQFMGHLKGVTDPEQKRKIIGREFVEVFQAEARKLPNARWLAQGTIY
                     PDVIESGGAKSKKAHTIKSHHNVGGLPETLGLKLLEPLRDLFKDEVRELGVALGLPHD
                     MVYRHPFPGPGLGVRILGEVRQDFADLLRRADAIFIDELRAADWYDKTSQAFAVFLPV
                     KSVGVMGDGRTYEYVVALRAVQTQDFMTAHWAELPHSLLGKVSNRIINEVRGINRVVY
                     DISGKPPATIEW"
     gene            complement(1635..3098)
                     /gene="guaB"
                     /locus_tag="PKN25_09465"
     CDS             complement(1635..3098)
                     /gene="guaB"
                     /locus_tag="PKN25_09465"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNV91168.1"
                     /translation="MRVIQKALTFDDVLLVPAHSTVLPRDVSLQSQVTRRIRLNVPLV
                     SAAMDTVTESRLAIALAQEGGIGVVHKNLTPAQQAAEVLKVKRHESGILKDPITIPPT
                     MTVGEVIALQRQNRFSGVPVVEGGKVVGIVTNRDTRFETKLDQLVSEIMTPQDRLVTV
                     REGASLEEARELLRVHRLERVLVLNDAGELCGLITVKDMMKSTEHPLAAKDEHGRLRV
                     AAAIGVGAGTEERAERLADAGVDMIVVDTAHGHSQGVLDRVGWVKKHFPHIEVVGGNI
                     ATADAARALVDAGADGVKVGIGPGSICTTRIVAGVGVPQISAIDNVANALLGTGVPMI
                     ADGGIRFSGDIAKAIAAGADVVMLGGLFAGTEEAPGETVLFQGRSYKSYRGMGSLGAM
                     EKGAADRYFQEENTGNIDKLVPEGIEGRVPYKGSVTAVIHQLVGGLRASMGYLGCADI
                     PTMHARAEFVQISSAGMRESHVHDVQITKEAPNYQIS"
     gene            3181..3681
                     /locus_tag="PKN25_09470"
     CDS             3181..3681
                     /locus_tag="PKN25_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNV91169.1"
                     /translation="MTHPFAPVAILALLLTALPAAAQVYSWKDKDGRIHYGDMPPAGV
                     EATVIRGARPGAAARKAGETTPSADMPPAATPTSPSSLAEREQAFRERRAAAAEAEAK
                     AAREAESRGETERFCTDAQHEIAALRGGQRMVRFNAKGEREFLDDRARAEQAEALERK
                     IAEHCR"
     gene            complement(3768..4094)
                     /locus_tag="PKN25_09475"
     CDS             complement(3768..4094)
                     /locus_tag="PKN25_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNV91170.1"
                     /translation="MSGMINVEVVYALPQRQELVHVRLAEGASVRDALEASGLLQKHP
                     EIQPDRANKLGIYARLVKLDTEVRDRDRVEIYRPLIADPKAVRKKRADEGKVMKKGGG
                     PAEAAE"
     gene            complement(4091..4543)
                     /locus_tag="PKN25_09480"
     CDS             complement(4091..4543)
                     /locus_tag="PKN25_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HNV91171.1"
                     /translation="MAEVNKLVLIEFTPAQMFELVDRCEEYPLFLPWCGGVDLIGRTE
                     TITAATLHINYHGIKAHFSTENAKRYPQEMKLRLTDGPFTHLDGHWRFTALGETACKV
                     EFQLRYQFASKLLEKVLGPVFNHIANTFVDSFVKRAGQVYRAGQGSAA"
     gene            4666..5112
                     /gene="smpB"
                     /locus_tag="PKN25_09485"
     CDS             4666..5112
                     /gene="smpB"
                     /locus_tag="PKN25_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNV91172.1"
                     /translation="MSIIENRKALHDYFIEEKHEAGIVLEGWEVKAIRAGRSNIKESY
                     VVIRAEEIFVFGMHITPLSTASSHVRHDPTRTRKLLLHKKEIARLIGKVERAGYTLVP
                     LDLHYKNGLVKLEIGLAKGKKLHDKREDEKKKDWEREKQRLMRVKA"
     gene            complement(5119..5691)
                     /locus_tag="PKN25_09490"
     CDS             complement(5119..5691)
                     /locus_tag="PKN25_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNV91173.1"
                     /translation="MDVMQFPRMVVSSRDGWDLVARERPPMDWLFRRLVLPMSLLPPL
                     MILLASGGVGARVFPDAGFGNWLIAAVLFFVAEHFSVPIMANSIRQASMAKDGSSRLE
                     DAYTVAAIAPVPLWLSSLAVVFGSAWFVVLVGLVALGASAMLIRHGVDRLLGIDEDVE
                     ASEVAMQVTSFGVLTWLVLLAVAGGAVTLL"
     gene            5869..6276
                     /locus_tag="PKN25_09495"
     CDS             5869..6276
                     /locus_tag="PKN25_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNV91174.1"
                     /translation="MNTPLDKSEFEALADFRYQLRRFLRFSELLTRRHGITNLQYLLL
                     LQVKGYPGREWACIGELAERLQAHQHGVVSLVSRCEKLGLVERRPGREDRRAVEVHLT
                     AEGERLAATIALHHREEVKLLGGVFRDLASQLR"
     gene            complement(6314..7045)
                     /locus_tag="PKN25_09500"
     CDS             complement(6314..7045)
                     /locus_tag="PKN25_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV91175.1"
                     /translation="MAEEKIRVLLVDDHTLFRSGIRSLLQSYVCFEVVGEAGDGREGI
                     RLAGQLRPDVVLLDNNMPGLSGREAARLLLEEVPETRVLMLTVSEDADDLIETLRAGA
                     CGYLLKNIQAETLVSAIKTAAQGESVVSPQMMGKLLSGVRSSASRAAMGGAIAPAPGA
                     LARSAPAAGAEPAELEKLSPREREILRFIARGQSNKEIARALELAESTVKIHVQNMLR
                     KLNLSSRVQAAVFAVENGLGQGPEG"
     gene            complement(7045..8988)
                     /locus_tag="PKN25_09505"
     CDS             complement(7045..8988)
                     /locus_tag="PKN25_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNV91176.1"
                     /translation="MFIRGSGLSVKITGMLLGFFLVALAAIALTLFISWRLEGAAAAI
                     NDAGSLRMRLYRVAHHLARADLHTSDRQGFAGDLRARVDDFDRILGVLRRGDPSRPLF
                     VPREAGIQQVLSGMSEEWSVQLRPILLAIAADPDPANLHRRGLEFDRAARDYVEDIND
                     FVLRMEQSYARNTNILRTSQVILVALAVIGTILLLRFFFLTVIRPVFELHEGVKRMGQ
                     EDFDARVPVLSQDEFGELSAGFNRMAAHLQNVYDTLEERVVAKTRSLEEKNRELEILY
                     EIVAFLREPNDVDTLCKGFLERVQKTMGASASSARLLDLESRNLCMIVHDGLGDDFLD
                     REEVLACGECVCGEAAQGSGLSLTLDVRNAALAVTRDACRRAGFRTVSATSITASKRA
                     LGVFNLYFDQSDAIGDADRQLLEALGQQLGIAIENQRLHARERELAVSEERNLLAREL
                     HDSIAQGLAFLNLQVQMLERALAENRAADVSTTVEMLRRGIQESYEDVRELLVHFRTR
                     VEQQDLDAAIAAALRRLAEQTGIATDLDVQGDGAPLDPEAETQVLYIIQEALSNVRKH
                     AGASTVSVGVRRGRDGLAVTVRDDGVGFAEDRDASDSEAHIGLQIMRERAMRIGGRFA
                     VRSSPGRGTEIRLELPRTNEVLA"
     gene            complement(9093..9785)
                     /gene="narI"
                     /locus_tag="PKN25_09510"
     CDS             complement(9093..9785)
                     /gene="narI"
                     /locus_tag="PKN25_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNV91177.1"
                     /translation="MSLTNLFFGVYPYIALTVFVIGSWIRYDNEQYTWKTDSSQLLSK
                     QGMMLASNLFHVGVIMIFFGHFAGLVLPHGLWLGLGISDLAHQWIAIIAGVIFGIMCL
                     VGGSMLWYRRMYNPRVRINSRRSDVFIITWLMATLILGLLTVPFSIGHAVKGDPAVML
                     ALAEWVKSILLLNPQPELMTKVDPIFRAHIFFGMTVFLVFPFTRLVHILTAPFSYLTR
                     AYQIVRTKRRLA"
     gene            complement(9796..10344)
                     /gene="narJ"
                     /locus_tag="PKN25_09515"
     CDS             complement(9796..10344)
                     /gene="narJ"
                     /locus_tag="PKN25_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HNV91178.1"
                     /translation="MQTYKLLAMLLDYPGSEIVGDLRAAVSEQGGARGLVRSVDTDQV
                     FTEAEHGSIAGFIDWMLAQDQTELEGRFVKTFDLTPEHSLHLTHHVFGDDKNRGPALI
                     DLSEFYKSYGLQHDEHEIPDYLPMMMEFVSQLGETEARVFLTDAVKVFNVLATNLEKA
                     ESPYAALVRVIENHSSLARAAA"
     gene            complement(10357..11928)
                     /gene="narH"
                     /locus_tag="PKN25_09520"
     CDS             complement(10357..11928)
                     /gene="narH"
                     /locus_tag="PKN25_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HNV91179.1"
                     /translation="MKIRAQFAMVFNLDKCIGCHTCSVTCKNVWTNRKGVEYAWFNNV
                     ESKPGIGYPKEWENQDKWKGGWTKLNGKLELKAGGRLKKIVQIFSNPNLPEIDDYYEP
                     FTYDYARLQNAPLSEAAPTARPVSQITGEKMDKITWGPNWEDDLAGEFASRARDKNFS
                     NMEKEMYKQFENTFHMYLPRICNHCINPACVASCPSGALYKREEDGIVLADQDRCRGW
                     RMCISGCPYKKVFFNWESSKAEKCVGCYPRVENGMPTVCSESCVGRIRYNGIILYDAD
                     KISAAASADNVQDLYQEHLDMFVDPFDPAVIEQAKKDGITDDWILAAQKSPIYKMAVQ
                     WKIAFPLHPEYRTLPMIWYVPPLSPVQSQIDQKALPTEADGVIPKASAMRLPVKYLAN
                     LMTAGKEQPIIDALNRMIAMRSYQRSVHVEGTPDTRALEAVGMTEDMAKEMYRYLAIA
                     NYEDRFVIPTSKEEMRLEDYHGFQGQAGFSFGSASSVGISPNSLFPERRKESMEPRKP
                     VPHADELHDVTGERY"
     gene            complement(11964..>14992)
                     /locus_tag="PKN25_09525"
     CDS             complement(11964..>14992)
                     /locus_tag="PKN25_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HNV91180.1"
                     /translation="WYNSTYLIITGANLPMTRTPDAHFATEVRYKGTKIVSMAPDYAE
                     YVKFADLWMPVKQGTDAAAFLAMGHVALKEFLVEKQDPYFTEYARKYTDLPMQVVLRK
                     HGEGYVSDRNLRASDFDNNLGESNNPEWKTIVFDKKSGRFIAPNGSIGFRWGEDGKWN
                     LLEKAGAGQAETEAELTCIGSQDEVVSVGFPHFVPGEPELLYRNVPARRLKLASGEEA
                     FVTSVFDMQVAQYGIDRGLGGANVASSYDDANVAYTPAWAAKQTGVKAADLERTGREF
                     AENASKTKGKSMVIMGAAINHWYHNDLAYRSIMNLLHMCGCVGQTGGGWAHYVGQEKL
                     RPQAGWAPIAFALDWQRPPRHMNSTSFWYFHTDQWRYETVDADSLLSPAGPCRNKGSS
                     LADYNVKAVRMGWLPSAPHFNRNPIELAEEAARAGATDEASVSKYVVEQLKSGALDVS
                     FADPDAEENWPRNLIVWRANLIGSSAKGHEYFLKHLLGAQNGVMQEGGAGNNCKEVKW
                     REHGPTGKLDLMVDINFRLNSTGAYSDIILPTATWYEKNDLNTTDMHPFIHPLGEAVT
                     PGWESKSDWQIFRTLAKTFTALGAKHLGTRKDVVSLPMQHDSPMELATPMGVGKDWKH
                     GECDPIPGKTMPMLKLVERDYAQIYNKYIALGPLMVKLGNNVKGIDWDTTQEYEELKK
                     CNYPVQVEGVSKGMPSLEEDIYVCDSVMRMAPETNGEVAHKSWSALSKKTGIDHHHLY
                     AGRHEDKITFRDIVAQPRKIITAPTWSGIESETVSYTAGYTNIHEHIPFRTLTGRAHF
                     YQDHEWFLDFGEGFCAYKPPVDLKAHEKVPDSVRNKPHLTLSWITPHSKWGIHSSYQD
                     NLRMLNLFRGGPYVWLSEEEAATIGIRDNDWVEAINANGATVARAVVSQRIPRGMAIM
                     YHAQEKNVNVPGSNTTGKRGGILNSVTRVIVKPTNMVGGYAQLAYSFNYYGTVGCQRD
                     EVVVIHKIEDQDIDWLERPLTPEREAQRNPVGIGKK"
BASE COUNT         2471 a         4918 c         4943 g         2660 t
ORIGIN      
        1 tcccactcga ttgtcgccgg cggcttgccc gagatgtcgt agaccacccg gttgatgccg
       61 cgcacctcgt tgatgatgcg gttgctgacc ttgccgagca ggctgtgcgg cagctccgcc
      121 cagtgcgcgg tcatgaagtc ctgcgtctgc accgcgcgca gtgccaccac gtactcgtag
      181 gtacggccgt cgcccatcac gcccacgctc ttcaccggca ggaagaccgc gaaggcctgg
      241 ctcgtcttgt cgtaccagtc ggcggcgcgc agctcgtcga tgaagatcgc gtcggcacgg
      301 cgcagcaggt cggcgaagtc ctggcggacc tcgccgagga tgcgcacgcc cagacccggg
      361 cccgggaagg ggtggcgata gaccatgtcg tgcggcaggc cgagggccac gccgagctcg
      421 cgcacctcgt ccttgaagag gtcgcgcagc ggctcgagca gcttcaggcc gagcgtctcg
      481 ggcaggccgc cgacgttgtg gtggctcttg atggtgtgcg ccttcttgct cttcgcgccg
      541 ccggactcga tcacgtcggg gtagatggtg ccctgggcca gccacctggc gttcggcaat
      601 ttcctggctt cggcctgaaa gacctcgaca aactcgcggc cgatgatctt gcgcttctgc
      661 tcggggtcgg tgacgccctt caggtgtccc atgaactgct cggtcgcatc gacatgcacg
      721 accttggcgt ggagccgccc ggcgaacatc tccatgacca gcttgccttc gttcaggcgc
      781 agcaggccgt ggtcgacgaa cacgcaggtg agctggtcgc cgatcgcgcg gtggatcagc
      841 gccgccgcca ccgaggagtc cacgccgccc gacaggccga ggatgacctc ctcgtcaccc
      901 acctgctcgc ggatcttctg caccgcttcc tcgatgtaat cgggcatgtt ccagtcgtgg
      961 ccgcagccgc agatgtcgtg cacgaagcgc ccgatcatct ccttgccctt gatcgtgtgc
     1021 gtgacctcgg ggtggaactg cacgccgtag aagccgcgcg cctcgtcggc catcgccgcg
     1081 atcggagtcg attcgttgct gccgatgatc ttgaagcccg ggggcagcgc ggtgaccttg
     1141 tcgccgtggc tcatccacac gtcgagcagg ccgtggccct cggcgttgct gcggtcctcg
     1201 atgccggtga acagcttcga gtgcccgcgc gcgcgcatct ccgcatagcc gaactcgcgc
     1261 ttggccgagc tctcgacctt gccgccgagc tggctggcca tcgtctgcat gccgtagcag
     1321 atgcccagca ccggcacgcc aagctcgaac accgcctgcg gtgcgcgcca gtcctcggcc
     1381 tcgtaaaccg agttgggccc gccggagagg atcacgccct gcgcgccgaa gttgcgcacg
     1441 aactcgtccg agacgtcgaa gggatgcagc tcgcagtaca cctgctgctc gcgcacgcgg
     1501 cgcgcgatga gctgggagac ctgggagccg aaatcgagga tgaggatctt ctggtgggcc
     1561 atggggcggg aatctcgaac gtcgaaaaag gaagggcggc ctcgcagccg cccggatgtc
     1621 tcgaaggagc gcgctcagct gatctggtag ttgggcgctt ccttggtgat ctgcacgtcg
     1681 tgcacatgcg attcgcgcat gccggccgag ctgatctgca cgaactccgc gcgcgcatgc
     1741 atggtcggga tgtcggcgca gccgaggtag cccatcgagg cgcgcaggcc gccgacgagc
     1801 tgatggatca ccgccgtgac cgagcccttg tagggcacgc gaccctcgat gccctcgggc
     1861 accaacttgt cgatgttgcc ggtgttctct tcctggaagt agcggtcggc cgcgcccttc
     1921 tccatcgcgc cgagcgagcc catgccgcgg tacgacttgt aggaacggcc ctggaacagc
     1981 acagtctcgc ctggcgcctc ctcggtgccg gcaaacagtc caccgagcat gaccacgtcg
     2041 gcgcccgcgg cgatcgcctt ggcgatgtcg cccgagaagc ggatgccgcc gtcggcgatc
     2101 atcggcacgc ccgtgcccag cagcgcgttg gcgacgttgt cgatcgcgct gatctgcggc
     2161 acgcccacgc cggcgacgat gcgggtggtg cagatcgagc ccggaccgat gccgaccttg
     2221 acaccgtcgg caccggcatc gaccagcgca cgcgcggcgt cggcggtggc gatattgccg
     2281 cccaccacct cgatgtgcgg aaagtgcttc ttgacccagc cgacgcggtc gagcacgccc
     2341 tgcgagtgac cgtgggcggt gtcgaccacg atcatgtcca cgcccgcgtc ggcgaggcgc
     2401 tccgcgcgtt cctccgtgcc ggcccccacg ccgatcgcag cggccacgcg caagcgacca
     2461 tgctcgtcct tggcagcgag cgggtgctcg gtggacttca tcatgtcctt gacggtgatg
     2521 agaccgcaca gctcgccggc atcgttgagc accagcacgc gctcgaggcg gtgcacacgc
     2581 agcagctcgc gcgcctcttc gaggctggcg ccttcgcgca cggtgacaag gcggtcctgc
     2641 ggggtcatga tttcggagac gagctggtcg agcttggtct cgaagcgggt atcgcggttg
     2701 gtgacgatgc cgaccacctt gccgccttcg accaccggca cgccggagaa gcggttctgg
     2761 cgttgcagtg cgatgacctc gcccaccgtc atcgtgggcg ggatggtgat cggatccttg
     2821 aggatgcccg actcgtggcg cttgaccttg agcacctcgg cggcctgctg ggccggggtg
     2881 aggttcttgt gcaccacgcc gatcccgcct tcctgcgcga gcgcgatcgc gagacggctc
     2941 tcggtcaccg tgtccatggc ggccgagaca agcgggacgt tgaggcggat gcggcgcgtg
     3001 acctggctct gcaggctcac gtcgcgaggg aggaccgtgg agtgggcggg aacgagaagg
     3061 acgtcatcga aggtcagcgc cttctggatc actcgcatgg cttttttcct ttcgaccaaa
     3121 tccgtattat acagagcgct cctgcaccac gtaagcggct cgccgcggag aattgtgccg
     3181 atgacccatc cgttcgcccc ggtcgcgatc ctcgccctgt tgctgaccgc ccttcccgcg
     3241 gccgcgcagg tctacagctg gaaggacaag gacgggcgca tccactacgg cgacatgcct
     3301 cccgcaggcg tcgaggccac ggtgatccgc ggagcacgac cgggtgccgc cgcgcgcaag
     3361 gctggagaga ccacgccgtc ggcggacatg ccgcctgctg ccactcccac ctccccgagt
     3421 tcgctcgccg agcgcgaaca ggctttccgg gaacgccgcg ccgccgcggc ggaggccgag
     3481 gcgaaggccg ctcgggaagc ggaatcgaga ggcgagacgg aacgtttctg cacggacgcg
     3541 cagcatgaaa tcgccgcctt gcgtggcggc cagcgcatgg tccgcttcaa cgccaagggg
     3601 gaacgcgagt tcctcgacga tcgtgcgcgc gcggagcagg ccgaggctct ggagcgcaag
     3661 atcgcagagc attgcaggta gatccgaccc gcaggtcgac gccagcaggc tcgcaagccc
     3721 aacgtcgcgc gagcggaaat tccgctgcgc cgggtcgcgc cgatccatca ctcggccgct
     3781 tcggcgggcc cacccccctt tttcatgacc ttgccctcgt cggcacgctt ctttcgcacc
     3841 gccttcgggt cggcgatcag cggacgatag atctccaccc ggtcgcggtc gcgcacctcg
     3901 gtgtcgagct tgaccagcct ggcatagatc cccagcttgt tcgcacggtc gggctggatc
     3961 tccggatgct tctgcaacag gccggaagcc tccagcgcat cgcgcaccga ggctccttcc
     4021 gccagccgca cgtgcaccag ctcctggcgc tgcggcagcg cgtacaccac ttccacgttg
     4081 atcatcccgc tcatgcagca ctcccctgcc cggcccgata gacctggccg gcacgcttga
     4141 cgaaggaatc gacgaaagtg ttcgcgatat ggttgaacac cggcccgagc accttctcca
     4201 gcagcttgct cgcgaactgg tagcgcagct ggaactcgac cttgcacgcg gtctcgccga
     4261 gcgcggtgaa gcgccagtgt ccgtcgagat gggtgaaggg gccgtcggtc aggcgcagct
     4321 tcatctcctg cgggtagcgc ttggcgttct cggtgctgaa atgcgccttg atgccatggt
     4381 aattgatgtg cagcgtagcc gcggtgatcg tctcggtgcg cccgatcaga tcgacgccgc
     4441 cgcaccaggg cagaaacagc gggtattcct cgcagcggtc gacgagctcg aacatctgcg
     4501 ccggggtgaa ttcgatcagg acgagcttgt tgacttcggc catgggcaag ttggtcggcg
     4561 ccaccaccat gacggaacgg aggtgcggcc gaactgttaa aatccgcaat tctaccgcat
     4621 acggacgcgc cggctcaggg ccgcgccgcc acgggacgcc gaaccatgag catcatcgaa
     4681 aaccgcaagg cccttcacga ctacttcatc gaggagaagc acgaagccgg catcgtcctc
     4741 gaaggctggg aggtcaaggc gatccgcgcc ggtcgctcga acatcaagga atcctacgtg
     4801 gtgatccgcg ccgaggagat ctttgttttc ggcatgcaca tcaccccgct cagcaccgcc
     4861 tccagccatg tgcgccacga cccgacgcgc acgcgcaaac tgctgctgca caagaaggag
     4921 atcgcccgcc tgatcggcaa ggtcgagcgc gccggctata cgctggtgcc gctcgacctg
     4981 cactacaaga atgggctcgt gaagctcgag atcgggctcg ccaagggcaa gaagctgcac
     5041 gacaagcgcg aggacgagaa gaagaaggac tgggagcgcg agaagcagcg cctcatgcgg
     5101 gtcaaggcct gagcgccctc acagcagcgt caccgccccc cccgcgaccg ccagcagcac
     5161 cagccaggtc agcacgccga agctggtcac ctgcatggcc acctcgctcg cctcgacgtc
     5221 ctcgtcgatg ccgagcaggc ggtcgacgcc gtggcggatc agcatggccg aggcgccgag
     5281 cgcgacgagg ccgacgagca ccacgaacca cgcgctgccg aacaccaccg cgagcgagga
     5341 cagccacagt ggcaccggcg cgatcgccgc caccgtgtag gcatcctcga gccggctgct
     5401 gccgtccttg gccatcgagg cctggcggat cgagttcgcc atgatcggca cgctgaagtg
     5461 ttcggcgacg aagaacagca cggccgcgat caaccagttg ccgaagcccg cgtcgggaaa
     5521 cacccgggcg cccacgcccc ccgatgccag caggatcatc agcggcggca gcagcgacat
     5581 cggcaggacc aggcgacgga acagccagtc catcggtggc cgttcgcgcg cgaccaagtc
     5641 ccagccatcg cggctcgaaa ccaccatgcg gggaaattgc atgacgtcca tgattcggtc
     5701 tcctctttcg gggtggaaat ccggccgccg ccacgatcca cctgcgcgcg tgggccatcg
     5761 ctctttttac atcacacttt gatattaatc cgcaatcgtc ggattgcaag gacgatctct
     5821 cgatcggact gccgaagcga gggcggaaga agctaaaatc cgcgcacgat gaacactcca
     5881 ctcgacaagt ccgaattcga ggccctcgcc gacttccgct accagctgcg gcgcttcctg
     5941 cgcttctccg agttgctcac ccggcgccac ggcatcacca acctccaata cctgctgctg
     6001 ctccaggtca agggctatcc cggacgcgaa tgggcctgca tcggcgagct cgccgagcgc
     6061 ctgcaggccc accagcatgg tgtggtgtcg ctggtttcgc gttgcgagaa actcggactg
     6121 gtcgagcgcc gcccgggtcg cgaggatcgg cgcgcggtcg aggtgcacct gaccgccgaa
     6181 ggcgagcgcc tcgcggcaac gatcgcactg caccaccgcg aggaggtcaa gctgctcggc
     6241 ggtgtgttcc gcgacctcgc ctcgcaattg cgctgaagcc ttcggaagcc cgctgcaaac
     6301 gcgccggcgg agctcagccc tccggtccct gccccaagcc gttctccacc gcgaataccg
     6361 ccgcctgcac ccggctgctc aggttgagct tgcgcaacat gttctgcacg tggatcttca
     6421 ccgtgctctc ggcgagctcg agtgcacggg cgatctcctt gttgctctgt ccgcgcgcga
     6481 tgaagcgcag gatctcgcgt tcgcgcggcg acagcttctc gagttcggcc ggctccgcac
     6541 cggcagcagg ggcactcctc gccagcgctc ccggcgcagg cgcgatcgca ccgcccatcg
     6601 ccgctcggct ggcgctgctg cgcacgccgc tcagcagctt gcccatcatc tggggcgaaa
     6661 ccaccgattc gccctgcgcg gccgtcttga tcgccgatac cagggtttcg gcctggatgt
     6721 tcttcagcag gtagccgcag gccccggcgc gcagcgtctc gataaggtcg tcggcgtcct
     6781 ccgagaccgt caacatcagc acacgcgtct cgggcacctc ctcgagcagc aggcgggcgg
     6841 cctcgcgccc ggagagcccg ggcatgttgt tgtcgagcaa caccacgtcg ggtcgcagct
     6901 gcccggcgag gcggatgccc tcgcgcccgt cgccggcctc gcccaccacc tcgaagcaga
     6961 cgtagctctg cagcagcgaa cggatgccgc tgcggaacag cgtgtggtcg tcgaccagaa
     7021 ggacacggat cttctcttca gccatcaagc cagcacctcg ttcgttctcg gcagttccag
     7081 tcggatctcg gtgccccgcc cgggcgacga gcgcaccgca aagcggccac cgatccgcat
     7141 ggcgcgctcg cgcatgatct gcaggccgat gtgcgcctcg ctgtcggacg cgtcacggtc
     7201 ttccgcaaag cccacgccgt cgtcgcgcac cgtcaccgcg agcccgtcgc gaccgcgccg
     7261 cacacccacg gaaacggtgc ttgcaccggc gtgcttgcgc acgttggaga gcgcttcctg
     7321 gatgatgtag agcacctggg tctcggcctc gggatcgagc ggtgcgccgt cgccctgcac
     7381 gtcgaggtcg gtggcgatgc cggtctgctc tgcgagccgg cgcagggctg cggcgatggc
     7441 ggcatcgaga tcctgctgct cgacccgggt gcggaagtgc accagcaact cgcgcacatc
     7501 ctcatagctc tcctggatac cgcgacgaag catctccacg gtcgtactca cgtccgcggc
     7561 gcggttctcg gcgagcgcgc gttccagcat ctgcacctgc aggttcagga aggccaggcc
     7621 ctgggcgatc gagtcgtgca gttcgcgcgc gagcaggttg cgctcttcgg agacggcgag
     7681 ttcgcgctcg cgcgcgtgca ggcgctggtt ctcgatcgcg atgccgagct gctgtccgag
     7741 agcctcgagc agctggcgat cggcgtcgcc gatcgcatcc gactggtcga aatacaggtt
     7801 gaacacgccg agcgcgcgct tgctggccgt gatcgaggtg gccgacaccg tacggaaccc
     7861 ggcgcgccga caggcatcgc gcgtcaccgc gagcgcggca ttgcgtacat ccagggtcag
     7921 cgaaagcccg ctgccctggg ccgcctcgcc gcagacgcat tcgccgcagg ccagcacttc
     7981 ctcgcgatcg aggaagtcgt cgcccagccc gtcgtggacg atcatgcaca ggttgcgcga
     8041 ttcgagatcg agcaggcgcg cggagctcgc actcgcaccc atcgtcttct gcacccgctc
     8101 gaggaagccc ttgcacagcg tgtcgacgtc gttgggttcg cgcaggaagg cgacgatctc
     8161 gtagaggatc tcgagctcgc ggttcttctc ttcgaggctg cgggtcttgg cgacgacacg
     8221 ctcctcgagc gtgtcataga cgttctgcaa gtgggctgcc atgcggttga agccggccga
     8281 gagctcgccg aactcgtcct gcgacagtac cggcacgcgg gcatcgaaat cctcctgccc
     8341 catgcgcttg acgccctcgt ggagctcgaa cacggggcgg atcacggtga ggaagaagaa
     8401 gcgcagcagc aggatggtgc cgatcaccgc gagggcaacc aggatcacct gcgaggtgcg
     8461 caggatgttg gtgttgcgcg catagctctg ctccatgcgc agcacgaaat cgttgatgtc
     8521 ctcgacgtag tcgcgggcgg cgcggtcgaa ctccaggccc ctgcgatgca ggttggccgg
     8581 atccgggtcc gcggcgatcg cgagcaggat cgggcgcagc tgcacgctcc attcttcgga
     8641 catcccggag agcacctgct ggataccggc ttcgcgcggc acgaacaagg ggcgggacgg
     8701 atctccccgg cgcagcacgc cgaggatgcg gtcgaagtcg tcgaccctcg ccctcaggtc
     8761 gccagcgaaa ccctgccggt cgctggtatg cagatccgca cgagcgaggt gatgcgccac
     8821 gcggtacaag cgcatccgca ggctgccggc atcgttgatc gcagccgccg cgccttccag
     8881 gcgccacgag atgaacagcg tcagcgcgat cgcggcaagt gccacgagga aaaagccgag
     8941 cagcatgccg gtgatcttga ccgacaggcc cgagccgcgg atgaacatga gaatcggtgc
     9001 tttctggcgc atccgcgcaa gctaggggca aaaggaccga aaggcaaggc cggcagcctt
     9061 gatggctgcc agccctgcct ctcgcttcag cctcaggcca ggcggcgctt ggtccggacg
     9121 atctggtagg cgcgcgtcag gtagctgaac ggcgcggtca ggatgtgcac caggcgggtg
     9181 aaggggaaca ccaggaacac cgtcatgccg aagaagatgt gggcacggaa gatcggatcg
     9241 accttcgtca tcagctcggg ctgcgggttc agcagcagga tgctcttgac ccactcggcc
     9301 agcgccagca tcacggccgg atcacccttg acggcgtgac cgatcgagaa cggcacggtc
     9361 agcaggccga ggatcagcgt ggccatcagc caggtgatga tgaacacgtc gctgcgacgg
     9421 ctgttgatgc gcacgcgcgg gttgtacatg cggcgatacc acagcatgga accaccgacc
     9481 aggcacatga tgccgaagat cacgccggcg atgatcgcga tccattggtg ggcgaggtcg
     9541 gagatcccca ggccaagcca caggccgtgc ggcagcacga gaccggcgaa atggccgaag
     9601 aagatcatga tgacgccgac gtggaacagg ttgctcgcca gcatcatgcc ttgcttggac
     9661 agcagctgcg acgaatcggt cttccaggtg tactgctcgt tgtcgtaccg gatccagctg
     9721 ccgatcacga acacggtcag cgcaatgtag ggatagaccc cgaaaaacag gttggtcagg
     9781 gacatcgtct tctcctcagg cggccgcgcg ggccagcgag ctgtggtttt cgatcacgcg
     9841 aacgagcgcc gcataggggc tctccgcctt ttccaggttg gtcgcgagca cgttgaacac
     9901 cttcaccgcg tcggtcagga acacgcgcgc ctcggtctcc ccgagttgcg acacgaactc
     9961 catcatcatc ggcaggtagt ccgggatctc gtgctcgtcg tgctgcagtc cgtaggactt
    10021 gtagaactcc gacaggtcga tcagcgccgg accgcggttc ttgtcgtcgc cgaacacatg
    10081 gtgggtcagg tgcaggctgt gctccggggt gaggtcgaag gtcttgacga aacggccttc
    10141 gagctcggtc tggtcctgag cgagcatcca gtcgatgaaa ccggcgatgc tcccgtgctc
    10201 agcctcggtg aacacctggt cggtgtccac cgagcgcacc agaccccgcg caccgccctg
    10261 ctccgagacc gcggcgcgca ggtcgccgac gatctcgctg cccgggtagt cgagcagcat
    10321 ggcaagcagc ttgtaggtct gcatggttca gtctcctcag tagcgttcgc cggtgacgtc
    10381 gtgcagctcg tcggcgtgcg ggaccggctt gcgcggctcc atcgactcct tgcggcgctc
    10441 ggggaacagc gagttcggcg agatgccgac cgacgacgcg ctgccgaagc tgaagcccgc
    10501 ctgaccctgg aagccgtggt agtcctcgag gcgcatctct tccttggagg tcggaatcac
    10561 gaagcggtct tcgtagttcg cgatcgccag gtagcgatac atctccttgg ccatgtcctc
    10621 ggtcatgccc accgcctcca gcgcgcgcgt gtcgggcgtg ccctcgacgt gcaccgagcg
    10681 ctggtaggag cgcatcgcga tcatgcggtt gagcgcgtcg atgatcggct gctccttgcc
    10741 cgcggtcatc aggttggcca ggtacttgac cggcaggcgc atcgcggagg ccttcgggat
    10801 aacgccgtcg gcctcggtcg gcagcgcctt ctggtcgatc tgcgactgca ccggcgagag
    10861 cggcggcacg taccagatca tcggcagcgt gcggtactcg gggtgcagcg ggaaggcgat
    10921 cttccactgc acggccatct tgtagatcgg cgacttctgc gcggcgagga tccagtcgtc
    10981 ggtgatgccg tccttcttgg cctgctcgat cacggccggg tcgaagggat cgacgaacat
    11041 gtccaggtgc tcctggtaca ggtcctggac gttgtcggcg ctggccgcgg ccgagatctt
    11101 gtcggcgtcg tacaggatga tgccgttgta gcggatgcgg ccgacgcagg actccgaaca
    11161 gacggtcggc atgccgttct cgacgcgcgg gtagcagccc acgcacttct cggccttgga
    11221 cgactcccag ttgaagaaca ccttcttgta cgggcagccc gagatgcaca tccgccagcc
    11281 gcggcagcgg tcctggtcgg cgagcacgat gccgtcttcc tcgcgcttgt agagcgcgcc
    11341 ggacgggcac gaggccacgc aagccgggtt gatgcagtgg ttgcagatgc gcggcaggta
    11401 catgtggaag gtgttttcga actgcttgta catttccttc tccatgttgg agaagttctt
    11461 gtcacgagca cgggaggcga actcgccggc gaggtcgtct tcccagttcg ggccccaggt
    11521 gatcttgtcc atcttctcgc cggtgatctg cgacactgga cgcgcggtcg gcgcggcctc
    11581 ggacagcggc gcgttctgca gacgggcgta gtcgtaggtg aagggctcgt agtagtcgtc
    11641 gatctcgggc aggttcgggt tggagaagat ctgcacgatc ttcttcaggc ggccgccggc
    11701 cttgagctcg agcttgccgt tgagcttggt ccagccaccc ttccacttgt cctggttctc
    11761 ccattccttc gggtaaccga tgcccggctt ggactcgacg ttgttgaacc aggcgtactc
    11821 gacgcccttg cggttggtcc acacgttctt gcaggtcacc gagcaggtgt ggcaaccgat
    11881 gcacttgtcg aggttgaaca ccatcgcgaa ttgcgcgcgg attttcattt gtttctcctg
    11941 aatctctggt ctcgagtcgg cgcttacttc ttgccgatgc ccacggggtt gcgttgggct
    12001 tcgcgttcgg gcgtgagcgg acgctccagc cagtcgatgt cctggtcctc gatcttgtgg
    12061 atcaccacga cttcgtcacg ctggcaaccg acggtgccgt agtagttgaa gctgtaagcc
    12121 agttgcgcat agccgccgac catgttggtg ggcttgacga tgacgcgggt gaccgagttc
    12181 aggatgccgc cacgcttgcc ggtcgtgttc gagccgggca cgttcacgtt cttttcctgg
    12241 gcgtgataca tgatcgccat gccacgcggg atgcgctgcg acaccaccgc acgggccacc
    12301 gtggcgccgt tggcgttgat cgcctcgacc cagtcgttgt cgcggatacc gatggtcgcg
    12361 gcctcttcct cggacagcca gacgtacggg ccgccgcgga acaggttgag catgcgcagg
    12421 ttgtcctggt agctggagtg gatgccccac ttcgagtgcg gggtgatcca cgacagggtc
    12481 aggtgcggct tgttgcgcac gctgtcgggc accttctcgt gggccttgag gtcgaccggc
    12541 ggcttgtagg cgcagaagcc ttcaccgaag tcgaggaacc actcgtggtc ctggtagaag
    12601 tgcgcacgac cggtcagggt acggaacgga atgtgctcgt ggatgttggt gtagcccgcg
    12661 gtgtaggaca cggtctccga ctcgatgccg gaccaggtgg gcgcggtgat gatcttgcgc
    12721 ggctgggcga cgatgtcgcg gaaggtgatc ttgtcctcgt gacggccggc gtacaggtgg
    12781 tggtgatcga tgccggtctt cttcgacagc gcgctccacg acttgtgcgc cacttcgccg
    12841 ttggtctcgg gcgccatgcg catgaccgag tcgcagacgt agatgtcttc ctcgagcgac
    12901 ggcatgccct tcgaaacacc ttcgacctgc accgggtagt tgcacttctt gagctcttcg
    12961 tactcctggg tggtatccca gtcgatgccc ttgacgttgt tgcccaactt caccatcagc
    13021 gggccgagcg cgatgtactt gttgtagatc tgcgcgtagt cgcgctcgac gagcttgagc
    13081 atcggcatgg tcttgcccgg gatcggatcg cactcgccgt gcttccagtc cttgcccacg
    13141 cccatcggag tcgcgagttc catcggcgag tcgtgctgca tcggcagcga gaccacgtcc
    13201 ttgcgggtgc cgaggtgctt ggcgccgagc gcggtgaagg tcttcgccag ggtgcggaag
    13261 atctgccagt cggacttcga ctcccagccc ggggtcaccg cctcacccag cgggtggatg
    13321 aaggggtgca tgtcggtggt gttgaggtcg ttcttctcgt accaggtcgc cgtcggcagg
    13381 atgatgtcgg agtacgcgcc ggtcgagttg aggcggaagt tgatgtccac catcaggtcg
    13441 agcttgccgg tcgggccatg ctcgcgccac ttcacttcct tgcagttgtt gcccgcgccg
    13501 ccttcctgca tcacgccgtt ctgcgcgccc agcaggtgct tgaggaagta ctcgtggccc
    13561 ttggccgacg agccgatcag gttggcgcgc cacacgatca ggttgcgcgg ccagttttcc
    13621 tcggcatccg ggtcggcgaa ggacacgtcg agcgcgcccg acttgagctg ttcgaccacg
    13681 tatttcgaca cgctcgcctc gtcggtcgca cccgcacgcg cggcttcttc ggcgagttcg
    13741 atcgggttgc ggttgaagtg cggggcggac ggcagccagc ccatgcgcac cgccttgacg
    13801 ttgtagtcgg cgagcgagga acccttgttg cggcagggac cggcgggcga cagcagcgag
    13861 tcggcatcca ccgtctcgta gcgccactgg tcggtgtgga agtaccagaa cgaggtcgag
    13921 ttcatgtggc gcggcggacg ctgccagtcg agcgcgaacg cgatcggcgc ccagcccgct
    13981 tgcggacgca gcttttcctg gcccacgtag tgcgcccagc caccaccggt ctgacccacg
    14041 cagccgcaca tgtgcagcag gttcatgatc gagcggtagg cgaggtcgtt gtggtaccag
    14101 tggttgatcg cagcgcccat gatcaccatg gacttgccct tcgtcttcga ggcgttctcg
    14161 gcgaactcgc ggccggtacg ctcgaggtcg gcggccttca cgccggtctg cttggcagcc
    14221 caggccgggg tgtaggcgac attggcgtcg tcgtaggacg aggccacgtt ggcgccaccc
    14281 aggccgcggt cgatgccgta ctgcgcgacc tgcatgtcga acaccgaggt cacgaaggct
    14341 tcttcgccgc tggcgagctt gaggcgacgc gccggcacgt tgcggtacag cagctcgggt
    14401 tcacccggca cgaagtgcgg gaagcccacc gagacgacct cgtcctggct gccgatgcag
    14461 gtgagctccg cttcggtctc ggcctggccg gcaccggcct tctcgagcag gttccacttg
    14521 ccgtcctcgc cccagcggaa gccgatcgag ccgttgggcg cgatgaagcg gccgctcttc
    14581 ttgtcgaaga cgatggtctt ccactcgggg ttgttggact cgccgaggtt gttgtcgaag
    14641 tcggaagcgc gcaggttgcg atccgaaaca tagccctcgc cgtgcttgcg cagcacgacc
    14701 tgcatcggca ggtcggtgta cttgcgcgcg tactcggtga agtacgggtc ctgcttctcg
    14761 acgaggaatt ccttcagcgc gacgtggccc atggccagga aggcggcggc gtcggtgccc
    14821 tgcttgaccg gcatccacag gtcggcgaac ttgacgtact cggcgtagtc cggcgccatc
    14881 gacacgatct tggtgccctt gtagcgcact tcggtggcga agtgggcgtc cggggtgcgg
    14941 gtcatcggca ggttggcgcc ggtgatgatc agataggtcg agttgtacca gt
//
LOCUS       DAOQBS010000073        14884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3088_length_14884_cov_16.020096, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000073 DAOQBS010000000
VERSION     DAOQBS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 14884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14884
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3088_length_14884_cov_16.020096"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /gene="infB"
                     /locus_tag="PKN25_09530"
     CDS             <1..1532
                     /gene="infB"
                     /locus_tag="PKN25_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNV91181.1"
                     /translation="AHKDADVLPRAPVVTVMGHVDHGKTSLLDYIRRAKVAAGEFGGI
                     TQHIGAYHVETARGMLTFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTREA
                     IHHAKAAGVPLVVAINKIDKPDANPERVTQELIAESVIPEAYGGDTMFVPVSAKKGTG
                     IDELLEAVLLQAEVLELTAPKDTPAKGLIIEARLDKGRGPVASLLVQSGTLRKGDVLL
                     AGATFGRIRAMLDENGKQIDEAGPSIPVEILGLSDVPSAGDEAIALADEKKAREIALF
                     RQGKFRDVKLAKQQAAKLETLMEQMSEGEVKSLALIIKADVQGSQEALVQSLQKLSTD
                     EVRVNVIHGAVGAISESDVNLAQASGAVIIGFNTRADAGARKLAETFGVDIRYYNIIY
                     DAVDEVKAALSGMLAPEKREEVTGLVEIRQVFTISKVGSIAGCYVLEGVVRRNSHVRL
                     LRNHTVLWTGELESLKRFKDDVKEVKFGYECGLQLRNYNDIQEGDQLEVFEIKEVART
                     L"
     gene            1536..1961
                     /gene="rbfA"
                     /locus_tag="PKN25_09535"
     CDS             1536..1961
                     /gene="rbfA"
                     /locus_tag="PKN25_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNV91182.1"
                     /translation="MPKEYSRSQRVVEQIRRELAELIRLEVKDPRVGFITLTDVEITP
                     DYAHAKVFFTNMTGEADVPEILQGLRRASGFLRRELGRRIRIHTIPELHFHYDRSVEE
                     GSRLSQLIDEVVKEDEARQHDEAGGGDNTGTDDDARSRG"
     gene            1939..2925
                     /gene="truB"
                     /locus_tag="PKN25_09540"
     CDS             1939..2925
                     /gene="truB"
                     /locus_tag="PKN25_09540"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNV91183.1"
                     /translation="MTLAPAAEVAAEPARPKRKGQPQRRIVRREVDGVLLLDKPQGMS
                     SNGALQTARRLLAAAKAGHTGTLDPMASGLLPLTFGEATKFSQMLLDADKIYEAVVRL
                     GVDTDSGDAEGKVLATRPVAVDRAALEAVLERFRGEIEQVPPMYSALKRDGKALYEYA
                     RAGIELERAARRVVIHALELLDFAGERFTIRVHCSKGTYIRSLAMDIGAALGCGAHLA
                     ALRRTAIGAFDLSGALTLEALEAAGEGGRDALLAPVDALVAGFPVLQLDAEAARGLLQ
                     GRTLALAGAQPGAKVRAYGPGGFLGLAQWQDDGRLAARRLIATGGRSEDASA"
     gene            3003..3272
                     /gene="rpsO"
                     /locus_tag="PKN25_09545"
     CDS             3003..3272
                     /gene="rpsO"
                     /locus_tag="PKN25_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNV91184.1"
                     /translation="MALDIASKSKIVSDFQRAQGDTGSPEVQIALLTARINGLAPHFK
                     EHAKDHHSRRGLLKMVSQRRKLLDYLKGRNADSYRNLIERLGLRK"
     gene            3491..5590
                     /gene="pnp"
                     /locus_tag="PKN25_09550"
     CDS             3491..5590
                     /gene="pnp"
                     /locus_tag="PKN25_09550"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNV91185.1"
                     /translation="MPTAIKKTFAYGAHTVTLETGEVARQAGGAVIVNMDETVVLATV
                     VGAKEARPGQDFFPLTVDYVEKFYAAGRIPGGFFKREGRPSEKEILTSRLIDRPIRPL
                     FPDGFMNEVQVIVTVLSLNPEIDSDIPAMIGASAALAISGLPFNGPVGAARVGYLDGK
                     YILNPTATELQSSAMNLVVAGTQTAVLMVESEAQELPEDVMLGAVVFGHEQMQTAIKA
                     INDLVEVAGKPEWDWQAPPANAALIAKVTELAKAEMEEAFRITSKQERSARLSEIRKR
                     VIGQLTEGVENAPSVNELKDLFFNLESGIVRSRILNGEPRIDGRDTRTVRPIEIRTGV
                     LPRTHGSALFTRGETQALVVATLGTGRDEQIIDAIAGEYKERFLLHYNFPPFCTGETG
                     RFGITKRREVGHGRLAKRSLVAMLPKPEEFSYTVRVVSEITESNGSSSMASVCGGSLA
                     MMDAGVPLKNHVAGIAMGLIKDGNRFAVLTDILGDEDHLGDMDFKVAGTENGITGLQM
                     DIKIQGITKEIMHAALEQAKEGRLHILGLMQQALGTSRGQVSEYAPRMINMKINPEKI
                     RDVIGKGGAVIRALQEETGTVIEIEDDGSVKISSVSAEGAQAAKARIEAITAEVEVGK
                     VYEGPVVRLLDFGAIVNIMPGRDGLLHVSQIANERVNNVSDYVKEGQVVRVKVLETDE
                     RGKIRLSMKALLNENAG"
     gene            complement(5708..6133)
                     /gene="dksA"
                     /locus_tag="PKN25_09555"
     CDS             complement(5708..6133)
                     /gene="dksA"
                     /locus_tag="PKN25_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNV91186.1"
                     /translation="MAEDTLHKQFPPYVPAAGEEYMSEKQLAHFRAILDSLKGELVDD
                     IERTVHTMQDEATVFADPNDRASQESDIALELRNRDRERKLIKKIDETIGRIEAGEYG
                     YCDSCGVEIGLKRLEARPTATLCIDCKTLEEMRERQVAK"
     gene            complement(6327..6857)
                     /locus_tag="PKN25_09560"
     CDS             complement(6327..6857)
                     /locus_tag="PKN25_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91187.1"
                     /translation="MASALVLTLAAAGCAPPTEEAALTPRGIGPMLLGVPLADAARAT
                     RRFDPAAARIGPGCDERDQIGVVLRVAGESLSVMAMAGADARIEEILAIPAQGAGAVT
                     LADAEACRAHGAAYAASLTPRLGAAPTWSVRERPVSHEFVFAFAGDVRVVARWFSGGR
                     SCDLLLQYGGKPAASP"
     gene            complement(6923..7453)
                     /locus_tag="PKN25_09565"
     CDS             complement(6923..7453)
                     /locus_tag="PKN25_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HNV91188.1"
                     /translation="MIKPAALAAALLATTLALPALAAERASARLLDRQGAEVGTATLV
                     EGPHGVLVHVVAKGLPPGPKAIHIHSVGTCEDGTQGFVASKGHLNPAARPHGLMNPDG
                     PDAGDLPNLFVHADGGVEAEFFTALASLSGAGGRAAILDADGAALVIHENRDDHASQP
                     IGGAGGRFFCGVIRAQ"
     gene            7605..9299
                     /locus_tag="PKN25_09570"
     CDS             7605..9299
                     /locus_tag="PKN25_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNV91189.1"
                     /translation="MASSPDSISMALFCDFENVALGVRDAKYEKFDIKRVLERLLLKG
                     SIVVKKAYCDWDRYKSFKAAMHEANFELIEIPHVRQSGKNSADIRLVVDALDLCYTKS
                     HVDTFVIISGDSDFSPLVSKLRENAKQVIGVGVKQSTSDLLIANCDEFIFYDDLVRDS
                     QRAAAKREARDNPPASRKQPEEDKARREELESRRSKAIDIAVETFDALLAERGESGKI
                     WSSMLKEAIKRRKPDFNESYFGFRAFGNLLEEAQARGLLELGRDEKSGIFVTRPGPGL
                     AAPRMREEFTMAGESLVVERAAPRTEPTVGVAEAEPGAEHASSPAGAGGETSANASEP
                     RRGRGRGRGRAARALEEPAAEGGAVEAHVAATVEEGETAIESAPAAPVLPAETVAQAT
                     SEGAGHAEDAAVAVEPRAAEAEPESAQPKRSSATRRRRGGKAEAPVLPAAAAATVAGA
                     DHPPAPAAANEAHDAVAGAAQEEKPKGKGKGKDKQKPKEKPSEAAKPSSRRGRGGRAA
                     GAKAAAEGAGGTATAAGPSASAAPLPSAAPAASTPAAENPAPRPRRARKPKAASAE"
     gene            9296..10024
                     /gene="ugpQ"
                     /locus_tag="PKN25_09575"
     CDS             9296..10024
                     /gene="ugpQ"
                     /locus_tag="PKN25_09575"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNV91190.1"
                     /translation="MSVPWPYPFWIAHRGAGKLAPENTLAAFALGHAHGYRMFECDAR
                     LSADGEVFLLHDDRLERTTDGRGEAVEQPWGALARLDAGAWHGPRHAGERLPTLAQVL
                     AFCGERGCLLNIEIKPAPGDEIRTGRRVAEVLAAASVVPPPLLSSFSPTALAAAAAVA
                     PATPRGLLLETFRAGWLDEARALGCVAVIAECAAWTPETAAQAAAAGLRRLAYTVNDA
                     AEAERLRALGLDGLITDRVDHFVP"
     gene            10100..11335
                     /locus_tag="PKN25_09580"
     CDS             10100..11335
                     /locus_tag="PKN25_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNV91191.1"
                     /translation="MSSSSWLRACALTSGLLCTLPTLANPAQSQAPSAAPACLAPASW
                     ARTDGVTPQVVDAGGLLTAMAGRAVVLLGEQHDDMDHHRWQLQTLSTLHALRPRMVIG
                     FEMFPRRAQPVLDRWVAGELTEAEFLQQSEWGKVWSMPAELYLPLFHFARMNRIPMLA
                     LNIDAELTRRIADKGWDGVPEGEREGVGRPAAAVSAYEDFLFEIHSQHAQMRQHGKDQ
                     GKPARGDAAFRNFVDSQLAWDRAMAEALLAGRARHAAADGSLPFAVGIMGSGHVRHGH
                     GVAHQLAALGEPSIGQLMPVEAATPCVELPAGLADAVFAVPALRQPPPPPPRLGVGLD
                     DTDGGIRIAELTPGSLAERSGLRRGDLITEAAGAPVRRSAQLIGLIRRQPAGTWLPLQ
                     VMREGKAVEVVVRFPREAR"
     gene            11337..13478
                     /locus_tag="PKN25_09585"
     CDS             11337..13478
                     /locus_tag="PKN25_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNV91192.1"
                     /translation="MLRGLFGWLLLAGWLGGLPSALAAAGPARLHLAVELDPARGELQ
                     VEAVLRAPEAGYRFVLHESLQPQAASADGHALPLRAAGSRGTLRAWRVDAPAGAELRL
                     RYGGKLPALEQARDHREVLQAMPPMASPAGSFLASGSGWYPRPADLFAYEVDLVVRGG
                     QPALVAGRLAHEQRPAAAGEPYRARFIFEHPADGIDLMAGPWVVRERLATQADGRPLR
                     LRTYFPAALDGEAGLAEAYLADSQRYIERYSAQIGAYPFTEFSVVASPLPTGFGMPTL
                     TYIGEQVLRLPFIRASSLGHEVLHNWWGNGVLVDYARGNWSEGLTTFMADYAYKAEAS
                     AAAAREMRLGWLRDFAALPADSHAPLADFRSRTHGAAAAVGYGKAAMVFVMLQDEIGE
                     DAFARGIRLFWERQRFRIAAWDELRAAFEEAAGRPLQGFFRQWLELPGGPAPRIERAR
                     LVEPAGQGARVRVSLAQPGPVHALRLPLQLVAGDRVETRVVEFAAGRTDVELTAGFVP
                     EGVALDPELRLWRLPEAAQLPPILRQWIVAAAPRLVLADAQAEAGGAGSGAVSGAGSG
                     AGSGVGSGASGGTPRGADSAFAEAAQALAARLFERTPTLAGLPALGAEGGPVLLVGSE
                     PAVAAVLVGAGLAAQPRGMPAGGSARVWTVQRERGPALAVIAAADPAALRALQRALPH
                     YGSQSWLVFDGTRASARGVWDAPGNVVKVEP"
     gene            13722..>14884
                     /locus_tag="PKN25_09590"
     CDS             13722..>14884
                     /locus_tag="PKN25_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HNV91193.1"
                     /translation="MSSRPLLLAAALAALSLPLSAAADEAAFASCLAKLRGEAAAKGV
                     HGETFDTHTAALAPDMAVIGFLDAQPEFVTPIWDYLAALVDEERVADGRAMLAQWKEV
                     LAEVERRYGVDAETVVAVWGVESNYGRNFGSRPLLTSLSTLSCFGRRQAFFRGEFFTT
                     LKILQEGHVAPERLTGSWAGAFGHTQFMPSTFMRLAVDFDGDGRRDLIDSVPDALAST
                     ANFLARAGWRSDLRWGFEVRLPRGMDTADAGRRNKQPMAEWAARGVRRADGSALPAAG
                     APAGLMLPAGREGPAFLVTRNFDALYSYNAAESYGLAIAHLSDRLRGGAPFATAWPTD
                     DPGLSRAERRELQRLLIARGHDIGEPDGMIGARTREALQREQAALGLPADGRAG"
BASE COUNT         2215 a         5288 c         5317 g         2064 t
ORIGIN      
        1 aagcgcacaa ggatgccgac gtgctgccgc gtgcgccggt ggtcaccgtg atgggccacg
       61 tcgaccacgg caagacctcg ctgctcgact acatccgccg cgccaaggtc gccgcgggcg
      121 agttcggcgg catcacccag cacatcggcg cgtatcacgt cgagaccgcg cgcggcatgc
      181 tcacgttcct cgacaccccg ggccacgagg cctttacggc gatgcgtgcc cgcggggcga
      241 aggcgaccga catcgtcatc ctggtggtcg cggccgacga cggcgtgatg ccgcagacgc
      301 gcgaggcgat ccaccacgcc aaggcggccg gcgtgccgct ggtggtggcg atcaacaaga
      361 tcgacaagcc cgacgccaac cccgagcgcg tgacccagga gctgatcgcc gagtcggtga
      421 tccccgaggc ctacggcggc gacaccatgt tcgtgccggt gtccgccaag aaggggaccg
      481 gcatcgacga gctgctcgag gccgtgctgc tgcaggccga ggtgctcgag ctcaccgcgc
      541 ccaaggacac gcccgccaag ggcctgatca tcgaggcgcg cctggacaag ggtcgtggcc
      601 cggtggcctc gctgctggtc cagtcgggaa cgctgcgcaa gggcgacgtg ctgctcgccg
      661 gcgccacctt cggccgcatc cgcgcgatgc tggacgaaaa cggcaagcag atcgacgagg
      721 ccggtccgtc catcccggtc gagatcctcg gcctgtcgga cgtgccgtcc gcgggtgacg
      781 aggcgatcgc gctggccgac gagaagaagg cgcgcgagat cgcgctcttc cgccagggca
      841 agttccgcga cgtcaagctc gccaagcagc aggccgccaa gctcgagacg ctcatggagc
      901 agatgtccga gggcgaggtc aagagcctgg cgctcatcat caaggccgac gtgcagggtt
      961 cgcaggaggc gctggtgcag tcgctgcaga agctctccac cgacgaagtc cgcgtcaacg
     1021 tcatccacgg tgcggtgggc gcgatcagcg aatccgacgt caacctggcg caggcctcgg
     1081 gcgcggtcat catcggcttc aatacccgtg ccgatgccgg ggcgcgcaag ctggccgaga
     1141 ccttcggtgt cgatatccgc tactacaaca tcatctacga cgccgtcgat gaggtgaagg
     1201 cggcgctgtc gggcatgctg gcgccggaga agcgcgagga agtcaccggc ctggtcgaga
     1261 tccgccaggt gttcacgatc tccaaggtcg gctcgatcgc gggttgctac gtgctcgagg
     1321 gtgtcgtgcg ccgcaattcg cacgtccgcc tgctgcgcaa ccacaccgtg ctgtggaccg
     1381 gcgagctcga gtcgctcaag cgcttcaagg acgacgtcaa ggaagtcaag ttcggctacg
     1441 agtgcggcct gcagctgcgc aactacaacg acatccagga aggcgaccag ctcgaggtct
     1501 tcgagatcaa ggaagtggcg aggaccctgt aagcgatgcc caaggagtat tcccgcagcc
     1561 agcgcgtggt ggagcagatc cgccgcgagc tggccgagct gatccgcctc gaggtgaagg
     1621 acccgcgcgt cggcttcatc accctgaccg atgtcgagat cacccccgac tacgcccacg
     1681 ccaaggtgtt cttcaccaac atgacgggcg aggccgacgt gcccgagatc ctgcagggct
     1741 tgcgccgcgc gagcggcttc ctgcgtcgcg agctcggccg ccgcatccgc atccacacca
     1801 tcccggagct gcacttccac tacgaccgtt cggtggaaga gggctcgcgc ctgtcgcagc
     1861 tgatcgacga ggtggtgaag gaagacgagg cccgccagca cgacgaggct ggcggcggcg
     1921 acaacaccgg cacggatgat gacgctcgct cccgcggctg aggtcgcggc tgagccggcg
     1981 cggccgaagc gcaagggcca gccgcaacgc aggatcgtcc gccgcgaagt cgacggcgtg
     2041 ctgttgctcg acaagccgca gggcatgagc tcgaacggtg ccttgcagac cgcacggcgc
     2101 ctgcttgcgg cggccaaggc cggccacacc ggcacgctgg atccgatggc gagcggactg
     2161 ctgccgctca ccttcggcga ggccaccaag ttctcgcaga tgctgctcga cgccgacaag
     2221 atctacgagg cggtggtgcg gctcggcgtg gacaccgaca gcggcgatgc cgagggcaag
     2281 gtgctcgcca cccgtccggt ggcggtggat cgcgccgcgc tcgaagccgt gctggagcgc
     2341 ttccgcggcg agatcgagca ggtgccgccg atgtattcgg cgctcaagcg cgacggcaag
     2401 gcgctctacg agtacgcgcg cgcgggcatc gagctcgagc gcgcggcgcg ccgggtcgtg
     2461 atccatgcgc tcgaactgct cgacttcgca ggcgagcgct tcacgatccg cgtgcattgc
     2521 agcaagggca cctacatccg cagcctcgcg atggacatcg gcgccgcgct cggttgcggt
     2581 gcccacctgg cagcgttgcg gcgcaccgcg atcggcgcct tcgacctgtc cggcgcgctc
     2641 acgctcgaag cgctggaggc ggccggcgag ggcggacgcg atgcgctgct cgccccggtc
     2701 gatgcgctgg tggcgggctt tccggtgctg cagctcgatg ccgaggcggc gcgcggcctg
     2761 ctgcagggtc gcacgctcgc gctcgcgggc gcgcagccgg gcgcgaaggt gcgcgcctac
     2821 gggccggggg gcttcctcgg cctggcgcag tggcaggacg acggacgcct ggcggcgcgc
     2881 cggctgatcg ccaccggtgg gcggagcgaa gatgcatccg catgattttg attgagtaac
     2941 tggcgcggct ttgggtataa tccgccgctt ctgattggca gaacacacga gtaaagacac
     3001 acatggctct cgatatcgca tccaagtcga agatcgtctc cgacttccag cgcgcccagg
     3061 gcgacaccgg ctccccggaa gtccagatcg cgctcctgac cgcccgcatc aacggtctcg
     3121 ccccccactt caaggagcac gcgaaggatc accactcccg ccgcggcctc ctgaagatgg
     3181 tcagccagcg ccgcaagctg ctggactacc tgaagggtcg caacgccgac agctaccgca
     3241 atctgatcga gcgtctgggc ctgcgcaagt aattgctgtc cgtgacgagt ccgacagcgc
     3301 cgatcagggc gaagtaagcc agcaaagccg gtgcgtgccc ctgcggccgc gccggctttt
     3361 tgccgttctc ccgccgcgtt cgcgcgcccg cccgagaccg tccctccgcg gtgcctggcc
     3421 gtccgctcgg acggcccgcc gcaagcagtt gtcgtttgct gcatccaccg aaagaacaag
     3481 gaataccaac ttgcctaccg caatcaagaa aaccttcgcc tacggcgccc acaccgtgac
     3541 cctggaaacc ggcgaagtcg cccgccaggc tggcggcgcc gtcatcgtga acatggacga
     3601 gaccgtggtg ctggccaccg tggtcggcgc caaggaagcc cgcccgggcc aggacttctt
     3661 cccgctcacc gtcgactacg tcgagaagtt ctatgccgcg ggccgtatcc ccggcggctt
     3721 cttcaagcgt gaaggccgcc cgagcgagaa ggagatcctc acctcgcgcc tgatcgaccg
     3781 tccgatccgc ccgctcttcc ccgacggctt catgaatgaa gtccaggtca tcgtcaccgt
     3841 gctgtcgctg aaccccgaga tcgactccga catcccggcg atgatcggtg cctcggccgc
     3901 gctggcgatc tccggcctgc ccttcaacgg cccggtcggc gccgcgcgcg tcggctacct
     3961 cgatggcaag tacatcctca acccgaccgc caccgagctg cagtcgagcg cgatgaacct
     4021 cgtcgtcgcc ggcacccaga ccgccgtgct gatggtcgag tccgaagccc aggagctgcc
     4081 cgaggatgtc atgctcggcg ccgtggtgtt cggccacgag cagatgcaga ccgccatcaa
     4141 ggccatcaac gatctcgtcg aggttgccgg caagcccgaa tgggactggc aggcgccgcc
     4201 cgccaatgcg gcgctgatcg ccaaggtcac cgagctcgcc aaggccgaga tggaagaggc
     4261 cttccgcatc accagcaagc aggagcgctc ggcccgcctg tccgagatcc gcaagcgcgt
     4321 gatcggccag ctcaccgaag gcgtggagaa cgcgccctcg gtcaatgaac tgaaggacct
     4381 cttcttcaac ctcgagtcgg gcatcgtccg cagccgcatc ctcaacggcg agccgcgcat
     4441 cgacggccgc gacacccgca ccgtgcgtcc gatcgagatc cgcaccggcg tgctgccgcg
     4501 cacccacggc tcggcgctgt tcacccgcgg cgagacgcag gccctggtgg tcgccaccct
     4561 gggcaccggc cgcgacgagc agatcatcga cgccatcgcc ggcgagtaca aggagcgctt
     4621 cctgctccac tacaacttcc cgcccttctg caccggcgag accggccgct tcggcatcac
     4681 caagcgccgc gaggtcggcc acggccgcct ggccaagcgc tcgctggtcg ccatgctgcc
     4741 caagccggaa gagttctcct acaccgtgcg cgtggtctcc gagatcaccg aatccaacgg
     4801 ctcctcgtcg atggcctcgg tgtgcggcgg ctcgctcgcc atgatggacg ccggcgtgcc
     4861 gctgaagaac cacgtcgccg gcatcgccat gggtctgatc aaggacggca accgcttcgc
     4921 ggtgctgacc gacatcctgg gcgacgagga ccacctcggc gacatggact tcaaggtcgc
     4981 cggtaccgag aacggcatca ccggcctgca gatggacatc aagatccagg gcatcaccaa
     5041 ggagatcatg cacgccgcgc tggagcaggc caaggaaggc cgcctgcaca tcctcggcct
     5101 catgcagcag gccctgggca ccagccgcgg ccaggtgtcc gagtacgctc cgcgcatgat
     5161 caacatgaag atcaaccccg agaagatccg cgacgtgatc ggcaagggcg gcgcggtgat
     5221 ccgcgcgctg caggaagaga ccggcaccgt gatcgagatc gaggacgacg gttcggtcaa
     5281 gatctcgtcg gtcagcgccg agggcgccca ggcggcgaag gccaggatcg aggcgatcac
     5341 cgccgaggtc gaggtcggca aggtctacga aggtccggtc gtgcgtctgc tcgacttcgg
     5401 cgccatcgtg aacatcatgc cgggccgcga cggcctgctg cacgtgtcgc agatcgccaa
     5461 cgagcgcgtg aataacgtct cggactacgt caaggaaggc caggtcgtgc gcgtgaaggt
     5521 cctcgagacc gacgagcgcg gcaagatccg cctgagcatg aaggcgctgc tgaacgaaaa
     5581 cgccggctga tggcggatgg cgggctgcgg cccgccacgc gctgcagcac ggcgtgcgga
     5641 caacaaaaaa ggacgcccca ggcgtccttt tttgttgtcc gtcccggtcg cgaccgggag
     5701 cggcggatta cttcgcgacc tggcgctcgc gcatctcttc cagcgtcttg cagtcgatgc
     5761 acaaggtcgc ggtcgggcgc gcctcgaggc gcttgaggcc gatctcgacg ccgcagctgt
     5821 cgcagtagcc gtattcgccg gcctcgatgc gaccgatcgt ctcgtcgatc ttcttgatca
     5881 gcttgcgctc gcggtcgcgg ttgcgcagct cgagcgcgat gtcggactcc tggctggcgc
     5941 ggtcgttggg gtcggcgaac acggtcgcct cgtcctgcat cgtgtgcacc gtgcgctcga
     6001 tgtcatccac cagctcgccc ttgagcgaat cgaggatcgc gcggaagtgg gccagctgct
     6061 tctcgctcat gtattcctcg ccggcagcgg gaacgtaggg cgggaattgc ttgtgcaggg
     6121 tatcttcagc catggatcga cttccgtaga gggtgttgat tggtgaggtc cgacttaata
     6181 acagaatcaa cccggtgcag caagcatacc ccggatcgcc ctggcgtgcc ccgtgtcgga
     6241 ccgcgcgttc ccggctgcga gtgcgcggct gccgcgctgc ggagccgcgg cggaggcgcc
     6301 gccagggccc cggacggtcg cggtgctcag gggctggcgg cgggtttgcc gccgtactgc
     6361 agcagcaggt cgcagctcct gccgccggaa aaccagcgcg ccaccacgcg tacgtcgccg
     6421 gcgaaggcga agacgaactc gtgcgagacc gggcgttcgc gcaccgacca ggtgggcgcc
     6481 gcgccgagcc ggggggtgag gctggccgcg taggcggcgc cgtgcgcacg gcaggcctcg
     6541 gcatcggcca gcgtgaccgc gccggcgccc tgcgcgggga tggcgaggat ctcctcgatc
     6601 ctggcgtcgg cgccggccat cgccatcacc gacagcgact cgccggccac acgcagcacc
     6661 acgccgatct ggtcgcgttc gtcgcagccc gggccgatcc gtgcggcggc aggatcgaag
     6721 cgccgggtgg cgcgggcggc gtcggcaagc ggaacgccga gcaacatcgg accgatgccg
     6781 cggggggtga gcgccgcctc ttcggtgggt ggcgcacagc ccgcggcggc gagcgtcagg
     6841 acgagcgccg acgccattgc gagggcggcg ggcgggtggg cgcgcgaccc ggcttcgcaa
     6901 cggctgaggc gatctgatcc gctcactgcg cccggatcac accgcagaag aagcgcccgc
     6961 cggcgccgcc gatcggctga ctggcgtggt cgtcgcggtt ctcgtggatc accagcgcgg
     7021 cgccgtcggc gtcgaggatg gcggcgcgtc cgcccgcgcc cgagaggctg gcgagggcgg
     7081 tgaagaactc cgcctcgacg ccgccgtcgg catgcacgaa gaggttgggc aggtcgccgg
     7141 cgtcggggcc atccgggttc atcagcccgt gcggccttgc cgccgggttc aggtggccct
     7201 tcgaggcgac gaagccctgc gtgccgtcct cgcaggtgcc caccgaatgg atgtggatcg
     7261 ccttcggccc gggcggcagt cccttggcga ccacgtggac caacacgccg tggggcccct
     7321 cgaccagggt cgcggtgccc acctcggcgc cctggcgatc gagcaaacgg gcgctcgcac
     7381 gctcggcggc gagggcgggc agggcgagcg tggtggcaag gagcgcggcg gcgagggcgg
     7441 cgggtttgat catggctgtt ctcctgttgg aatgcgtcca gagtctcaag cataggacaa
     7501 accggcaggc atgcacgccc caaggcgggc cgcggcggta catccggtgg cacgcaccgc
     7561 ggcgagggct taacatcggc ggctcatcag aaacggagat ttccatggct tcctcccccg
     7621 acagcatcag catggcgctg ttctgcgact tcgagaacgt ggcgctgggc gtgcgggacg
     7681 cgaagtacga gaagttcgac atcaagcggg tgctcgagcg cctgctgctc aagggcagca
     7741 tcgtggtcaa gaaggcctat tgcgactggg accgctacaa gagcttcaag gcggcgatgc
     7801 acgaggccaa cttcgagctc atcgagatcc cccacgtgcg ccagtccggc aagaattcgg
     7861 ccgacatccg gctcgtggtc gacgcgctcg acctgtgcta caccaagtcg catgtcgaca
     7921 ccttcgtgat catcagcggc gactcggact tctcgccgct ggtgtccaag ctgcgcgaga
     7981 acgccaagca ggtgatcggc gtcggggtca agcagtccac ctccgacctg ctgatcgcca
     8041 actgcgacga gttcatcttc tacgacgacc tcgtgcgcga cagccagcgc gccgcagcca
     8101 agcgcgaggc gcgcgacaac ccgcccgcca gccgcaagca gcccgaggag gacaaggccc
     8161 gccgcgagga gctcgagtcg cgccgcagca aggcgatcga cattgccgtc gagaccttcg
     8221 acgcgctgct cgccgaacgc ggcgagagcg gcaagatctg gtcctcgatg ctcaaggagg
     8281 cgatcaagcg ccgcaagccc gatttcaacg agagctactt cggcttccgc gccttcggca
     8341 acctgctcga ggaagcgcag gcgcgcggcc tgctggaact cggccgcgac gagaagtcgg
     8401 gcatcttcgt cacccgcccc ggcccggggc tggccgcacc ccgcatgcgc gaggagttca
     8461 ccatggcggg cgagtccctg gtggtggagc gtgccgcccc gcgcaccgag ccgaccgtgg
     8521 gcgtcgccga ggcggagccc ggcgcggagc acgccagctc gcctgccggc gcgggcgggg
     8581 agacttccgc caacgcttcg gagccgcggc gcggtcgcgg tcgcggtcgc ggccgcgcgg
     8641 cacgagcgct ggaggagccc gcggctgaag ggggggcggt cgaggcgcat gtcgccgcca
     8701 cggtggagga gggcgagacc gccatcgaaa gcgcgccggc agcgcctgtc ttgccggcgg
     8761 agacggtggc gcaggcgacc tccgagggcg ccgggcacgc cgaggacgcc gcggtggcgg
     8821 tcgagcctcg cgcagccgag gccgagcccg agtccgcgca gccgaagcgc tcgtccgcga
     8881 cgcgcaggcg gcgtggcggc aaggccgagg cgcccgtcct gccggccgcc gctgcggcca
     8941 ccgtcgccgg cgcggaccat ccgcctgctc ctgccgcggc gaacgaagcc cacgacgccg
     9001 tggccggcgc ggcgcaggag gagaagccga agggcaaagg caaggggaag gacaagcaga
     9061 agccgaagga gaaaccctcc gaggccgcaa agccgtcttc gcggcgtggt cgcggtgggc
     9121 gcgctgcggg tgccaaggcc gctgcggaag gggcgggcgg gacggcgacc gcggcgggcc
     9181 cgtcggcgag cgccgcgccc ctgccgagcg ccgcgcccgc cgcgagcacg cccgcggcgg
     9241 agaaccccgc cccgcgtccg cgccgcgcgc gcaagccgaa ggccgcgtcg gccgaatgag
     9301 cgtgccctgg ccgtatccgt tctggatcgc ccatcgcggc gccggcaagc tggcgccgga
     9361 gaacacgctc gccgcgttcg cgctcggcca tgcgcacggc taccgcatgt tcgagtgcga
     9421 tgcccgcctg agcgcggacg gcgaggtctt cctgctccac gacgaccgcc tcgagcgcac
     9481 caccgacggc cgtggcgagg cggtggagca gccctggggc gcactcgcgc gcctcgacgc
     9541 gggcgcctgg cacggccccc gccacgccgg cgagcgcctg cccacgctcg cgcaggtgct
     9601 ggccttctgc ggcgagcgcg gctgcctgct gaacatcgag atcaagcccg cacccggcga
     9661 cgagatccgc accggacgcc gggtggccga ggtcctggcc gcggcgtccg tcgtcccgcc
     9721 gccgctgctg agctcctttt ctcccaccgc gctcgccgct gcggccgcgg tggcgccggc
     9781 cacgccgcgc gggctgttgc tcgagacctt ccgtgccggc tggctcgacg aagcgcgtgc
     9841 gctcggctgc gtcgcggtga tcgcggagtg cgcggcgtgg acgccggaga ccgcggcgca
     9901 ggccgccgcg gccggcctgc gccgcctggc ctacaccgtg aacgacgccg ccgaggcgga
     9961 acgcctgcgc gcgctcggcc tcgacgggct gatcaccgac cgcgtcgacc acttcgtccc
    10021 ctgagcggcc cggcgcggga gcggaactcg cgtgcgcgcg cgggctctac ttgacgtttc
    10081 gtctcccgag cccacgatca tgtcctcttc ctcctggctg cgcgcgtgcg cgctcacgag
    10141 cggtctcctg tgcacgctgc cgacgctggc gaacccggcg cagagccagg ctccgtccgc
    10201 cgcgcccgcc tgcctcgcgc ccgcgagctg ggcgcgcacc gacggggtga cgccgcaggt
    10261 ggtggacgcg ggcgggctgc tgaccgcgat ggccgggcgc gcggtcgtgc tgctcggcga
    10321 gcagcacgac gacatggacc accaccgctg gcagctgcag accctctcca cgctgcatgc
    10381 gctgcgccca cggatggtga tcggtttcga gatgttcccg cgccgggcgc agccggtgct
    10441 cgaccgctgg gtggcgggcg agctgaccga ggcggagttc ctgcagcagt cggaatgggg
    10501 caaggtgtgg agcatgccgg cggagctcta cctgccgctc ttccacttcg cgcgcatgaa
    10561 ccgtatcccg atgctcgcgc tcaacatcga cgcggagctc acccggcgga tcgccgacaa
    10621 gggctgggac ggcgtgcccg agggcgagcg cgagggcgtc ggtcggccgg ccgcggcggt
    10681 gtcggcttac gaggacttcc tcttcgagat ccacagccag cacgcccaga tgcgccagca
    10741 cggcaaggac cagggcaagc cggcgcgcgg cgatgccgct ttccgcaact tcgtggattc
    10801 ccagctcgcc tgggaccgtg cgatggccga agccttgctc gccggccgcg cgcgccacgc
    10861 cgccgcggac ggcagcctgc cgttcgcagt gggcatcatg gggagcgggc atgtgcgcca
    10921 cggccacggc gtggcccatc agctcgccgc gctcggcgag ccgagcatcg gccagctgat
    10981 gccggtcgag gcggcgacgc cttgcgtgga actccccgcc ggcctcgccg acgcggtgtt
    11041 cgccgtaccg gcgctgcgcc agccgccgcc tccgccgccg cggctcgggg tggggctcga
    11101 cgacaccgac ggcggcatcc gcatcgccga gctcacgccg ggcagcctcg ccgagcgcag
    11161 cggcctgcgc cgcggcgacc tgatcaccga ggcggcgggt gcgccggtgc ggcgctccgc
    11221 gcagctgatc ggcctgatcc gccgccagcc ggcgggcacc tggttgccgc tgcaggtgat
    11281 gcgcgaaggc aaggcggtcg aggtggtggt gcgcttcccg cgcgaggcgc gctgagatgc
    11341 tgcgcgggct gttcgggtgg ctgctgcttg ccggctggct gggcgggctt ccttccgccc
    11401 tggccgcggc cgggccggcc aggctgcatc tggcggtcga gctcgatccg gcgcgcggcg
    11461 agcttcaggt cgaggccgtg ctgcgtgcgc ccgaagccgg ctaccgcttc gtgttgcacg
    11521 aatccctgca gccgcaggcc gcgagcgccg acgggcatgc gctgccgctg cgtgccgcgg
    11581 gcagccgcgg tacgttgcgc gcctggcggg tggatgcgcc ggccggcgcc gagctgcgcc
    11641 tgcgctacgg cggcaagctg cccgcgctcg agcaggcacg cgaccaccgc gaggtgctgc
    11701 aggccatgcc gccgatggcc tcgccagcgg gcagcttcct cgccagtggc agcggctggt
    11761 atccgcgtcc ggccgacctc ttcgcctacg aggtggacct cgtggtccgt ggcggccagc
    11821 cggcgctggt cgccggacga ctggcgcacg agcagcgccc cgccgcggcg ggcgagccct
    11881 accgcgcgcg cttcatcttt gaacatccgg cagacggcat cgacctgatg gccgggccct
    11941 gggtggtgcg cgaacgcctc gccacccagg ccgacggccg gccgctgcgc ctgcgcacct
    12001 actttcccgc cgcgctcgac ggcgaggccg ggctggccga ggcctacctc gccgacagcc
    12061 agcgctacat cgagcgctac tcggcacaga tcggcgccta ccccttcacc gagttctcgg
    12121 tggtggcgag cccgctgccg accggcttcg gcatgcccac gctgacctac atcggcgagc
    12181 aggtgctgcg cctgcccttc atccgcgcga gctcgctcgg ccacgaggtg ctgcacaact
    12241 ggtggggcaa cggcgtgctg gtcgactacg cgcgtggcaa ctggtcggag gggctgacca
    12301 ccttcatggc cgactacgcc tacaaggccg aggcgtcggc cgccgcggcg cgcgagatgc
    12361 gcctgggctg gctgcgcgac ttcgccgcgc tgccggcgga ttcgcacgca ccgctcgccg
    12421 atttccgctc gcgcacccac ggcgccgcgg ccgcggtggg ctacggcaag gcggcgatgg
    12481 tcttcgtgat gctgcaggac gaaatcggcg aggacgcgtt cgcgcgcggc atccgccttt
    12541 tctgggaacg ccagcgcttc cgtatcgccg cttgggacga actgcgcgcc gccttcgagg
    12601 aggcagccgg gcgcccgctg cagggcttct tccgccagtg gctggagctg cccggcgggc
    12661 cggcgccgcg catcgaacgg gcacgcttgg tggagccggc cgggcagggc gcgcgcgtgc
    12721 gcgtgagcct ggcgcaaccg ggaccggtgc acgccttgcg gctgccactg cagctcgtgg
    12781 cgggggatcg ggtagagacc cgggtcgtcg agttcgccgc gggccgcacc gatgtggagc
    12841 tgaccgccgg cttcgtgccc gagggcgtgg ccctcgaccc ggagctgcgt ctgtggcgcc
    12901 tgcccgaggc cgcgcaactg ccgcccatcc tgcgccagtg gatcgtcgcc gccgcgccgc
    12961 ggctggtgct tgccgacgca caggcggagg cgggcggggc ggggagcggc gcagtgagtg
    13021 gcgcagggag tggcgcaggg agtggcgtag ggagtggcgc aagcggtggc acgccgagag
    13081 gggcggacag tgcattcgcc gaggcggcgc aggcgctcgc cgcccgcctg ttcgagcgca
    13141 cgccgacgct ggcaggtctc ccagccttgg gcgcagaggg cgggccggtg ctgctggtgg
    13201 gcagcgagcc cgcggtggcc gcggtgctcg tcggtgccgg cctggcggca cagccccggg
    13261 gcatgcccgc aggcggcagc gccagggtgt ggaccgtgca gcgtgagcgc ggcccggcgc
    13321 tcgccgtgat cgccgccgcc gaccccgccg cgctgcgcgc gctgcagcgc gcgttgccgc
    13381 actacggcag ccagagctgg ctggtcttcg acggcacgcg ggcaagcgcc cgcggcgtat
    13441 gggacgcgcc tggcaatgtg gtgaaggtgg agccttgagc agccctcgcg aagctcgcag
    13501 tcccgcttcc acgcccgagg agcggcgtag gagcgacgca agtcgcgatc agtgcgcccg
    13561 tggttcgcgc aggcgcagtt cgaccgcagc aatcgcgact tgcgtcgctc ctacgggagg
    13621 gggcacgacg ctgcgggcgc ctgcagccgc cgcgcggcga tccgcctcct cgcggtgcta
    13681 gactgcggcg cccatccgca atcgcataac aggccttgtc catgtcttcc cgtcctctcc
    13741 tgctcgccgc cgcgctcgcg gcgctctcgc tgcccctgtc tgccgctgcc gacgaggccg
    13801 cgttcgcgtc ctgcctcgcc aagctgcgcg gcgaggccgc ggccaagggc gtgcatggcg
    13861 agaccttcga cacccacacg gccgcgctcg cgcccgacat ggcggtgatc ggcttcctcg
    13921 acgcgcagcc cgagttcgtc accccgatct gggactacct cgccgcgctg gtggacgagg
    13981 agcgcgtcgc cgacgggcgc gcgatgctgg cgcagtggaa ggaggtgctc gccgaggtcg
    14041 agcgccgcta cggcgtggat gccgagaccg tggtcgcggt gtggggcgtg gagagcaact
    14101 acgggcgcaa cttcggcagc cggccgctgc tgacttcgct gtccacgctg tcctgcttcg
    14161 ggcgccggca ggccttcttc cgcggcgaat tcttcaccac gctgaagatc ctgcaggagg
    14221 gccacgtcgc ccccgagcgc ctcaccggct cctgggcggg cgccttcggc cacacccagt
    14281 tcatgccctc gaccttcatg cgcctggcgg tggacttcga cggcgacggt cgccgcgacc
    14341 tcatcgacag cgtacccgac gcgctcgcct cgaccgccaa cttcctcgcg cgcgccggct
    14401 ggcgcagtga cctgcgctgg ggcttcgagg tccgcctgcc gcgcggcatg gacaccgccg
    14461 acgccggccg gcgcaacaag cagccgatgg ccgaatgggc cgcgcgcggc gtgcggcggg
    14521 ccgacggcag cgccttgccc gcggccggcg cgccggccgg gctgatgctg ccggcggggc
    14581 gcgaaggccc ggccttcctg gtcacgcgca acttcgacgc gctgtattcc tataacgccg
    14641 cggagagcta cggcctcgcc atcgcgcatc tgtcggaccg cctgcgcggc ggcgcgccct
    14701 tcgccaccgc ctggccgacc gacgaccccg gcctgtcgcg tgccgagcgc cgcgagctgc
    14761 agcggctgct gatcgcgcgc ggtcacgaca tcggcgagcc cgacggcatg atcggcgcgc
    14821 gcacccgcga ggcgctgcag cgcgagcagg ccgcgctcgg cctgccggcc gacggccgcg
    14881 ccgg
//
LOCUS       DAOQBS010000074        14741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3132_length_14741_cov_14.110241, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000074 DAOQBS010000000
VERSION     DAOQBS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 14741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14741
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3132_length_14741_cov_14.110241"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PKN25_09595"
     CDS             <1..1125
                     /locus_tag="PKN25_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNV91194.1"
                     /translation="GISLFVVPKFMVNADGSLGARNDVHCVSIEHKLGIHASPTAVLA
                     FGDKGGAIGTLVGEPNRGLEYMFIMMNEARFAVGMEGLALSERSYQHALQYAKDRIQG
                     TDAGVRGGPKVNILHHADVRRLLMNMKSKTEAMRALAYVVGAAFDKAHHHADEAVRAQ
                     NQAFVDLMIPVVKGWCTENSIDVTSDGVQVHGGMGFIEETGAAQHFRDSRITTIYEGT
                     TAIQANDLIGRKIARENGATVGAVVKMMRAVEAEVAGVQDEAFAAIARALGKGIAAVE
                     EAVAYILATYSQDIKAASVGSVPFLKLLGIVAGGWQMARAALVAKRKLEAGEGDAGFC
                     KAKIVTARFYADHVLAQAGGLAYTVVNGAPGALELTEELF"
     assembly_gap    1158..1257
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1304..2086)
                     /locus_tag="PKN25_09600"
     CDS             complement(1304..2086)
                     /locus_tag="PKN25_09600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV91195.1"
                     /translation="MKHFPSRLAITLLAGFLALPALAASPVAKVNGVAIPGERSDAML
                     AEQRAQGAPDGEQLRNAVREELIRREVLSQEAGKRGLDKKADVKAQMEMARQAILIRA
                     YLQDYVRTNPVDDAELKKEYEAIKGRMGDKEYKPRHVLVETEDQAKAIIARLQGGTAF
                     EEVAKESRDPGSKERGGELGWSNPAMYVKPFSDAMVQLQKGKYTTTPVKSDFGYHVIQ
                     LDDVRDVQPPPFEEVKPQLQQRLQQQKIERHVLDLREKAKVE"
     gene            complement(2097..2384)
                     /locus_tag="PKN25_09605"
     CDS             complement(2097..2384)
                     /locus_tag="PKN25_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNV91196.1"
                     /translation="MSATRVERIRAALQERFAPTRLEIGDDSALHAGHAGAREGGGHY
                     RVFIVSEAFQGCSRVVRQRMIYDAVGDMMHKDIHALAIRALTAAEAESQGH"
     gene            complement(2381..2680)
                     /locus_tag="PKN25_09610"
     CDS             complement(2381..2680)
                     /locus_tag="PKN25_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNV91197.1"
                     /translation="MLYALIGEDAPGALETRLAVRPQHVARLEALRDDGRLLLAGPMP
                     AIDSPDPGPAGFAGSLIVAEFDSLASAEAWLRDDPYVRQGVFVRTTVRPFRKTLP"
     gene            complement(2680..3288)
                     /locus_tag="PKN25_09615"
     CDS             complement(2680..3288)
                     /locus_tag="PKN25_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HNV91198.1"
                     /translation="MKFLFDLLPVILFFAAYKIGGANPEASHAFAAGWLGDGIAVSQA
                     PILIATFVAILATFLQIALVWLKHRKVDTMLWISLAIIVVFGGATLFFHNPTFIKWKP
                     TALYWLFGVVLLGSASLLGRNLIRTMLEAQIKLPDPVWARLNLAWAGFFIAMGVLNLW
                     VAYNFSEEAWVDFKMFGGMGLMLAFVLAQGFYLSRHMEEESN"
     gene            complement(3285..4700)
                     /locus_tag="PKN25_09620"
     CDS             complement(3285..4700)
                     /locus_tag="PKN25_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV91199.1"
                     /translation="MAGTVEAAGGPARPRLLPSTLLWQTFLLVALLLIVALGSWVQIF
                     RYFQEPARSRDVAQMVVSVINLTRTALINADFDRRMDLLIDLAALEGIRIYPAEATDE
                     LAPLADTRPLRLLTADVRRQLGDHTRFASRWKTLDGFWVSFRLDPDDANDEYWVMLPP
                     ERIENPDDFGWLGWGGGALLAALLGAFLIVARVSAPLRQLARAARMVGSGERPPPQPE
                     SGPQEIALVARAFNQMAGNLARMDEDRALILAGVSHDLRTPLARLRLGIEMSGAPDDE
                     VTAMVADIGEMDRIIGQFLDFGRGDAQEPTAAIDLATLAREVTEPYRLRGVDLRLEVP
                     ESLFVQARALSIRRALANLIDNALRYAGGNAPLEVTVSIEDGQARIEVADRGPGIPES
                     EIERMRQPFTRLEKARSNTKGAGLGLAIVDRVMRAHQGRLELSARDGGGLRAALCLPL
                     AAPAGEQDRMRRSAHSEPDRP"
     gene            complement(4700..5473)
                     /gene="ompR"
                     /locus_tag="PKN25_09625"
     CDS             complement(4700..5473)
                     /gene="ompR"
                     /locus_tag="PKN25_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HNV91200.1"
                     /translation="MNQKNRYRILVVDDDARLRELLSRYLQEQGFSVKAVGDAPMMDR
                     ALHREHYDLIVLDLMLPGEDGLSICRRLRAAENQIPVIMLTAKGDDVDRIVGLEMGAD
                     DYVAKPFNPRELVARIQAVMRRQPHTLPGAPTPEDEIVAFGRVKVNLGTRVLVRDGEE
                     IQLTTGEFSLLKVLLTHPRQPLSRDKLMELARGREYGVFDRAIDVQVSRLRKLVEDDP
                     AKPRYIQTVWGFGYVFVPDEQKGGEDNGGQPDTPAGGED"
     gene            5801..6073
                     /locus_tag="PKN25_09630"
     CDS             5801..6073
                     /locus_tag="PKN25_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91201.1"
                     /translation="MLAFFVFAPTTASAQLRFIVGGEEQAQRLRPAGAEDRQEWRDTL
                     SDQRNEREGARERRRMSEEERSDLRRHMRDAARGAYREESGRKPQR"
     gene            6206..6898
                     /gene="tsaB"
                     /locus_tag="PKN25_09635"
     CDS             6206..6898
                     /gene="tsaB"
                     /locus_tag="PKN25_09635"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNV91202.1"
                     /translation="MNILSIETATEHGSVALLHGDELLVRRIQGAANHSEAVLRDLRE
                     LLAETGLAVARLDAVAFGAGPGAFTGLRLACGVAQGIALAADLGVAVVGSLQALALQV
                     GAPRVLAATDARMGEIYWASFVTDTEGVPQPQGMPRCCAPLDLELPPGEWSAAGSAFR
                     AWPEELEARTAGRLVACQPDLHPRAEEIARLGAIQAREGGLVAPEQAAPLYVRDKVAF
                     TTAERLARGGRA"
     gene            6898..7344
                     /gene="rimI"
                     /locus_tag="PKN25_09640"
     CDS             6898..7344
                     /gene="rimI"
                     /locus_tag="PKN25_09640"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNV91203.1"
                     /translation="MLALAFAPMGEGDLDWVGAREAELHAHPWSRGNFADSLASGYDA
                     WILSCDGVPSGYAVVMNVLDEAHLLDIGIVGELQGRGLGKRFLDWLAEGAKTRGARDF
                     YLEVRPSNAAALRLYARSGFVEIGRRRGYYPAAGGREDAIVMRRAL"
     gene            7344..8258
                     /locus_tag="PKN25_09645"
     CDS             7344..8258
                     /locus_tag="PKN25_09645"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNV91204.1"
                     /translation="MSPRLSSRRDAVLREMGLGPIWRLRTRKAEVSEADDAEREAAEP
                     ASTETDATASPRVEVPRTPNPPPAAPASAAGPRSSVAFQAGPHRAARPQPADASTPAA
                     VRIAPAAPDPARGARISTLEWDALEAEIRDCKACGLCERRKQAVPGVGDRQARWMLVG
                     EAPGAEEDQRGEPFVGQAGRLLDNMLAAIGLKRGEDVYIANAVKCRPPHNRTPERGEI
                     AACQPYLDRQIALVQPQLLVALGRPAAQALLDREIAISAARGKRFERAGTPVVVTYHP
                     AYLLRNPQDKAKAWEDLCFARRLIAETG"
     gene            complement(8307..9557)
                     /gene="lplT"
                     /locus_tag="PKN25_09650"
     CDS             complement(8307..9557)
                     /gene="lplT"
                     /locus_tag="PKN25_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HNV91205.1"
                     /translation="MPLGFYIIMAAQFFSALADNALLIIAIAMLRDMAAPSEYEPLLK
                     LFFTVSYVALAAFVGAFADSMPKWRVMLISNTIKIGGCLMLFLGTHPLFAYAVIGLGA
                     AAYSPAKYGILTEYLPHRLLVVANGWIEGLTVAAIIVGTVIGGIMIRGDVSTWLMGLG
                     LPGAGSAFGAAVLVVGTLYLVAAIFNMYIPDTGVDHKPLKSNPLYLLHDFNHCLRLLW
                     RDKLGQISLAVTTLFWGAGATLQFIVIKWAEHNLGMNLSQASMLQGVVALGVATGAVL
                     AARYITLRRAVNVIPLGIAMGLGVMIMVGVTEAWVAMALMVIVGGLAGFFVVPMNALL
                     QHRGHILMGAGHSIAVQNFNENLSILMMTGTYALLIVLGLSINTVIVLFGLFVAGTML
                     LVKRRHEANQREFDNVALLEDRPH"
     gene            9775..10686
                     /locus_tag="PKN25_09655"
     CDS             9775..10686
                     /locus_tag="PKN25_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV91206.1"
                     /translation="MADRRLQVFHAVAKHGSFTRAAEHLHMTQPAVTFQIKQLEEHFD
                     TRLLDRGHGKITLTAAGELVLAYAERILGLSAELDSRVSELTDELAGQLRIGTSTTIA
                     AYWLPQVLESFKRRYPGVLPRVVVGTSKLTEDRVAARDLDLGLIEIATEQPSIERRSA
                     ARDDLLVICSPGHPLAKFKELTARDLVGHPFIDRDPGNGIRQIADEFFEAAGIAGSQI
                     TLCAELGSLAAVKQLAAAGLGFAIASGRAIRRDVEEGRLVAIPLQPRLHTPLEVILPR
                     DKFRSRLINAFADHVCEEFARIATEQD"
     gene            10748..12166
                     /gene="radA"
                     /locus_tag="PKN25_09660"
     CDS             10748..12166
                     /gene="radA"
                     /locus_tag="PKN25_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNV91207.1"
                     /translation="MARQKSAFVCTECGAQAVRWQGQCPQCQAWNTLVETVLEPAAPA
                     AGSRFAALAGETSRLQPLSALEPREEPRQPTGLDEFDRVLGGGLVAGGVVLIGGDPGI
                     GKSTLLLQALSNLAATSASAGSEGATGKGTKAGGVIYVSGEESGEQVALRAQRLQLPQ
                     SPLQMLAEINLERILHTLREARPRVAVIDSIQTVYSETLQSAPGSVAQVRECAAQLTR
                     FAKQSGTSLILVGHVTKDGTLAGPRVLEHIVDTVLYFEGDTHSSFRLIRAFKNRFGAV
                     NELGVFAMTERGLRGVSNPSAIFLSQHSQQVAGSCVLITQEGTRPLLVEVQALVDSSQ
                     SPNPRRLSVGLEQNRLAMLLAVMHRHAGVVCFDQDVFVNAVGGVKITEPAADLAILFA
                     IVSSLRDRPLRRGLAVFGEVGLAGEIRPAPRGQERLKEAAKLGFDTAIVPRANAPKHP
                     IEGLKVIAVERVEEALEQLRGL"
     gene            complement(12395..12838)
                     /locus_tag="PKN25_09665"
     CDS             complement(12395..12838)
                     /locus_tag="PKN25_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNV91208.1"
                     /translation="MSAPVLVFGWGNPSRGDDALGPLFVEAVEAMKLPGIECLTDFQL
                     QVEHALDLRRRSRILFVDASGTARPPFEFQPIAAARDDSFTTHSVSPQSVLQVFEDLE
                     GRPAPPSWLLAIRGDSWELGTAPAPSANANLRAAIAAFTAWLPDL"
     gene            complement(12835..14301)
                     /locus_tag="PKN25_09670"
     CDS             complement(12835..14301)
                     /locus_tag="PKN25_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNV91209.1"
                     /translation="MSFQLETATVASDTLRRIAIDPVSRVEGHGKVTILLDEDNKVHQ
                     VRLHIVEFRGFERFIQGRPYWEVPVMVQRLCGICPVSHHLAASKALDLVVGAARVTPT
                     ADKMRRLMHYGQILQSHALHFFHLSSPDLLFGFGSELGRRNIVGVAQQYPDIAKKGIL
                     LRKFGQEVIRLTAGKRVHGTGAVPGGVNRALSADERKLLLADAYQMIAWSRDAVRIIK
                     ELHQQDRALYDHFGTVRSSFMGLVAPDGSLDLYHGVLRARDADGRILFDQVDYQTYDR
                     LITEEVKPWSYMKFPYFTSMGPEAGWYKVGPLARVQNCDSIPTPFAEHERRELVAYAG
                     GKPLHGSLAFHWARMIEMLFAAETIKDLLHDDDLSGTELMTSGERRREGVGVIEAPRG
                     TLFHHYQVGDDDLVTMANLIVSTTNNNQAMNTAVREVAKQYLHGQEITEGLLNHIEVA
                     IRAFDPCLSCATHALGQMPLEVTLVDAEGTELDRKYKA"
     gene            complement(14410..>14741)
                     /locus_tag="PKN25_09675"
     CDS             complement(14410..>14741)
                     /locus_tag="PKN25_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNV91210.1"
                     /translation="EFRANCKVLIALGACAVNGGLPAQRNHLELADLLQEVYRTGHGL
                     AEGSRIPDDPELPLPLNQVHPVHEVVRIDYFLPGCPPSGDAIWAFLTQLIEGKDPSLG
                     HGLLHYD"
BASE COUNT         2288 a         4973 c         5080 g         2300 t
ORIGIN      
        1 ggcatctcgc tgttcgtcgt gcccaagttc atggtcaacg ccgacggcag cctgggcgcg
       61 cgcaacgacg tgcattgcgt gtcgatcgag cacaagctcg gcatccacgc cagccccacc
      121 gccgtgctcg ccttcggcga caagggcggc gcgatcggta ctctggtcgg cgagcccaac
      181 cgcggcctcg agtacatgtt catcatgatg aacgaagccc gcttcgcggt cggcatggaa
      241 ggcctcgcgc tgtccgagcg ctcttaccag cacgcgctgc agtacgccaa ggatcgcatc
      301 cagggcaccg acgccggcgt gcgcggcggt ccgaaggtca acatcctgca tcacgccgac
      361 gtgcgtcgcc tgctcatgaa catgaagagc aagaccgagg ccatgcgtgc actcgcctac
      421 gtcgtcggcg ccgccttcga caaggcgcat caccacgccg acgaggctgt gcgtgcgcag
      481 aatcaggcct tcgtcgacct gatgatcccg gtggtcaagg gctggtgcac cgagaactcg
      541 atcgacgtga cctcggacgg cgtgcaggtg cacggcggga tgggcttcat cgaggagacc
      601 ggcgcggcgc agcacttccg cgattcgcgc atcaccacga tctacgaggg cacgaccgcc
      661 atccaggcca acgatctgat cggccgcaag atcgcccgtg agaacggcgc caccgtcggt
      721 gccgttgtga agatgatgcg cgcggtcgag gccgaagtgg ccggcgtgca ggacgaggcc
      781 ttcgcagcca tcgcgcgcgc gctcggcaag ggcatcgcgg ccgtggaaga ggctgtggcc
      841 tacatcctcg ccacctactc ccaggacatc aaggcggcct cggtcggttc ggtgcccttc
      901 ctgaagctgc tcggcatcgt ggctggcggc tggcagatgg cgcgcgccgc gctggtggcc
      961 aagcgcaagc tcgaggcggg cgagggcgat gccggcttct gcaaggccaa gatcgtcacc
     1021 gcgcgcttct atgccgacca cgtgctcgcg caggccggcg gtctggccta cacggtcgtc
     1081 aatggcgccc cgggcgcgct ggagctgacc gaagagctgt tctgaccgga tattgcccgg
     1141 cgtggaagcg ccggagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
     1261 ccttccacgc aggagcgtcg gaaggcgcgg acaggacggg cgcttactcg accttggcct
     1321 tctcgcgcag atcgagcacg tggcgctcga tcttctgctg ctgcaggcgt tgctgcagtt
     1381 gcggcttgac ctcctcgaac ggcggcggtt gcacgtcgcg cacgtcgtcg agctggatca
     1441 cgtgatagcc gaaatcgctc ttgacgggcg tcgtcgtgta tttgcccttc tggagctgga
     1501 ccatcgcatc cgagaacggc ttcacgtaca tggcggggtt gctccagccg agctcgccgc
     1561 cgcgctcctt ggagcccggg tcgcgcgact ccttggcgac ttcctcgaag gcggtaccgc
     1621 cctgcaggcg ggcgatgatc gccttggcct ggtcttcggt ctcgaccagc acgtggcggg
     1681 gcttgtattc cttgtcgccc atgcggccct tgatggcctc gtattccttc ttcagctcgg
     1741 cgtcgtcgac cgggttcgtg cgcacgtagt cctgcaggta ggcacggatc aggatggcct
     1801 ggcgggccat ctccatctgc gccttcacgt ccgccttctt gtccaggccc ctcttgccgg
     1861 cttcctggct cagtacctcg cggcggatca gttcctcgcg caccgcattg cgcagctgtt
     1921 caccgtcggg cgcgccctgg gcgcgctgct cggccagcat ggcgtcggaa cgctcgccgg
     1981 gaatcgcgac tccattgacc ttggcaaccg gggatgcggc gagggcgggc agcgcgagga
     2041 agccggcaag cagggtgatg gcgagacggc tcgggaaatg tttcatggag attccttcaa
     2101 tgtccttgtg attcggcctc tgcggccgtt agcgcgcgga tggcgagggc atggatgtcc
     2161 ttgtgcatca tgtccccgac cgcgtcgtag atcatacgct gccggacgac tctgctgcag
     2221 ccctggaaag cttccgagac gatgaaaacc cgataatgtc cgcctccctc gcgcgcaccc
     2281 gcgtgcccgg cgtgcagcgc gctgtcgtcg ccgatctcga ggcgtgtcgg tgcgaagcgt
     2341 tcctgcagcg cggcgcggat gcgctcgacc ctcgtcgcgc tcacggcagc gtcttccgga
     2401 acgggcgcac ggtggtccgt acgaacacgc cctggcgcac gtaggggtcg tcccgaagcc
     2461 aggcctcggc gctggcgagc gagtcgaact cggcgacgat caggctgccg gcgaagcccg
     2521 ccgggccggg gtcgggcgag tcgatcgcgg gcatcggccc ggccagcaac aggcggccgt
     2581 cgtcgcgcaa tgcctcgagg cgggcgacat gttgcgggcg caccgcgagg cgcgtctcga
     2641 gcgcgccggg agcgtcctct ccgatcaggg cgtagagcat cagttgcttt cctcttccat
     2701 gtgacgggac aggtagaaac cctgggcgag cacgaaggcg agcatcagcc ccatgccgcc
     2761 gaacatcttg aagtcgaccc aggcttcttc gctgaagttg taggcgaccc aaaggttgag
     2821 cacgcccatc gcgatgaaga aaccggccca ggcgaggttg agccgcgccc aaaccgggtc
     2881 cggtagcttg atctgggcct ccagcatggt gcggatgagg ttgcgtccga gcagcgaggc
     2941 cgagccgagc agcaccacgc cgaacagcca gtagagcgcg gtcggcttcc acttgatgaa
     3001 ggtggggttg tggaagaaga gggtggcgcc gccgaatacg acgatgatcg ccaggctgat
     3061 ccacagcatg gtgtccacct tgcggtgctt gagccacacc agtgcgatct gcaggaaggt
     3121 ggcgaggatg gcgacgaagg tcgcgatcag gatgggcgcc tgcgagaccg cgatgccgtc
     3181 gcccagccag ccggcagcga aggcatgcga ggcttcgggg ttggcgccgc cgatcttgta
     3241 ggcggcgaag aaaaggatca ccggcaggag atcgaagagg aatttcatgg tcggtccggt
     3301 tcgctgtgcg cggagcgccg cattctatcc tgttccccgg cgggagccgc gagcggcagg
     3361 cagagcgccg cgcgcagacc gccgccatcg cgggcggaca gctcgaggcg gccctggtgg
     3421 gcgcgcatca cccgatccac gatcgccagg cccaggccgg cgcccttcgt gttgctgcgc
     3481 gccttctcga gccgggtgaa gggctggcgc atgcgctcga tctcgctctc ggggatgccc
     3541 ggtccgcggt cggcgacctc gatgcgtgcc tgcccgtcct cgatggagac cgtcacctcc
     3601 agcggcgcgt tcccgcccgc gtagcgcagg gcgttgtcga tcaggttggc gagcgcgcgc
     3661 cggatcgaca gggcgcgcgc ctgtacgaac aggctctccg ggacctccag gcgaagatcc
     3721 acgccgcgca accggtacgg ttcggtgacc tcgcgcgcca gggtggcgag atcgatggcc
     3781 gcggtgggtt cctgcgcgtc gccgcggccg aagtcgagga actggccgat gatgcgatcc
     3841 atttcgccga tgtccgcgac catcgcggtg acctcgtcgt cgggcgcacc cgacatctcg
     3901 atacccaggc gcaggcgcgc gagcggcgtg cgcaggtcgt gggagacgcc agcgaggatc
     3961 agcgcgcggt cctcgtccat gcgcgcgagg ttgcccgcca tctggttgaa ggcgcgcgcc
     4021 accagcgcga tctcctgcgg gccgctctcg ggctgcggag gcgggcgctc gccgctgccc
     4081 accatgcgcg ccgcgcgcgc gagctggcgc aggggcgcgc tcacccgggc aacgatcagg
     4141 aaggcgccga gcagggcggc gagcagcgcg cccccccccc agccgagcca gccgaaatcg
     4201 tccgggttct cgatacgctc gggcggcagc atcacccagt attcatcgtt ggcgtcgtcc
     4261 ggatcgagac ggaagctcac ccagaagccg tccagcgtct tccagcgcga ggcgaagcgg
     4321 gtatggtcgc cgagctgccg acgtacgtcg gcagtgagca agcgcaaggg ccgggtgtcg
     4381 gcgagcggcg cgagctcgtc ggtggcctcg gcgggataga tgcggatgcc ttccagggcg
     4441 gcgaggtcga tcagcagatc catgcgacgg tcgaagtcgg cattgatcag cgcggtgcgg
     4501 gtgaggttga tcaccgaaac caccatctgc gccacgtcgc gcgagcgcgc cggctcctgg
     4561 aagtagcgga agatctgcac ccagctgccg agggcgacga tcagcagcag cgcgacgagc
     4621 aggaaggtct gccagagcag ggtgctcggc agcaggcggg gccgcgccgg cccgcccgct
     4681 gcctcgaccg tgcccgccat cagtcctcgc cgccggccgg ggtgtccggc tgcccgccgt
     4741 tgtcctcgcc gcccttttgc tcatcgggca cgaagacgta gccgaaaccc cacacggtct
     4801 ggatgtagcg cggcttggcg ggatcgtcct cgaccagctt gcgcaggcgc gagacctgga
     4861 cgtcgatcgc ccggtcgaac acaccgtact cgcgcccgcg cgcgagttcc atcaacttgt
     4921 cgcgggacag cggctggcgc gggtgggtta gcagcacctt gagcagcgag aattcgccgg
     4981 tggtgagctg aatctcctcg ccatcgcgga caagcacgcg ggtgcccagg ttcaccttga
     5041 cccggccaaa cgcgacgatc tcgtcctcgg gggtgggcgc gcccggcagg gtgtggggct
     5101 ggcgacgcat caccgcctgg atacgcgcca ccagctcacg cgggttgaag ggcttcgcga
     5161 cgtagtcgtc ggcgcccatc tccagcccga cgatgcgatc cacgtcgtca cccttggcgg
     5221 tgagcatgat gaccgggatc tggttctcgg ccgcgcgcag ccggcgacag atcgacagcc
     5281 cgtcctcgcc gggcagcatc aggtcgagca cgatcaggtc gtaatgctcg cggtgcaggg
     5341 cgcgatccat catcggcgca tcgccgacgg ccttcaccga aaagccttgt tcctgaaggt
     5401 atcgggacag caactcgcgc aggcgcgcgt cgtcatccac cacgagaatg cgatagcggt
     5461 ttttctggtt catgaacgga atgctagcct gactcgcggc agggtgccag ccttcccggg
     5521 gccggtcgcg gggggctccc ggtaagcctt cgtaaggcgg ggacggagag gcaatatcgg
     5581 cttacaaagc agtcgcgatc aggcccgcga aggctttgcg caccgggcgg tttgccgtaa
     5641 ggtgcggtca tcgctttcct gcgtcggttc cgtgcctcct cgaggccgta ggggccgacg
     5701 tcgtgctctc atgacccctt tccgcgtttc ccgcgccttc cggcatcccg cgcagcacct
     5761 tgcctgccgg ccgtgcgtgc gcgcttccct cgtttgcggc ttgctggcgt tcttcgtctt
     5821 cgcgcccacc acggccagcg cgcagctacg cttcatcgtc ggtggagagg agcaggcgca
     5881 acgcttgcgt ccggcgggag ccgaggaccg gcaggaatgg cgcgacaccc tgagcgacca
     5941 gcgcaacgag cgcgagggcg cgcgcgagcg ccggcgcatg agcgaggagg agcgcagcga
     6001 cctgcgccgc cacatgcgcg acgccgcgcg cggcgcctac cgcgaggaat cggggcgcaa
     6061 gccgcagcgc tgagcactcg gcaatccggc agtcgggata cccgcccccg catgcggtcg
     6121 cggtcttcgt tcgcccctgt cgatgccgcc gcgtgacaac cgcggccgca acgaaccggc
     6181 gcgctcccgg tagaatggcg gccccatgaa catcctctcc atcgaaaccg ccaccgaaca
     6241 cggcagcgtc gcgttgctgc atggtgacga gctgctcgtc cgccgcatcc agggcgcggc
     6301 caatcactcc gaggcggtgc tgcgcgacct gcgcgaactc cttgccgaga ccgggctcgc
     6361 cgtggcgcgg ctggacgcgg tcgccttcgg cgccggtccc ggtgccttca ccgggctgcg
     6421 gctggcttgc ggtgtcgcgc aaggcatcgc gctggcagcg gatctcggcg tggccgtggt
     6481 cggcagcctg caggcgctcg cgctccaggt cggcgcgccg cgggtgctgg cggccaccga
     6541 tgcgcgcatg ggcgagatct actgggcgag tttcgtgacc gacaccgaag gggtgccgca
     6601 gccgcagggc atgccgcgct gttgcgcgcc gctggatctc gagctgcccc ccggcgagtg
     6661 gagcgctgcc gggtcggcct tccgcgcctg gccggaggaa ctcgaggcgc gcactgcagg
     6721 gcgtctcgtc gcctgccagc ccgaccttca cccgcgtgcg gaagagattg cccgcctcgg
     6781 ggcgatccag gcgcgcgaag gcgggctggt ggcccccgag caggccgcgc cgctctacgt
     6841 gcgcgacaag gtcgccttca ccaccgccga gcgactcgcg cgcggagggc gtgcctgatg
     6901 ctggcgctgg cctttgcgcc gatgggcgag ggagacctcg actgggtcgg cgcgcgcgag
     6961 gcggagctcc atgcccatcc ctggtcgcgc ggcaatttcg ccgactcgct ggccagcggc
     7021 tacgacgcct ggatcctgtc ctgcgacggt gtcccttccg gctacgcggt cgtgatgaac
     7081 gtgctcgacg aagcgcatct gctcgacatc ggcatcgtcg gcgagctgca gggcaggggg
     7141 ctgggcaagc gcttcctcga ctggctggcc gaaggggcga agacgcgcgg cgcgcgggat
     7201 ttctacctcg aggtgcggcc ttccaacgcc gcggcgctgc gcctctacgc gcgcagcggc
     7261 ttcgtcgaga tcggtcgccg gcgcgggtat taccccgcgg cgggtgggcg cgaggacgcg
     7321 atcgtgatga ggcgggcgct gtgatgtcgc cgcgcctgtc ctcccgccgc gacgcggtgc
     7381 tgcgcgagat gggactcggc ccgatctggc gcctgcgcac gcggaaggcg gaggtatccg
     7441 aagcggatga tgcggagcgt gaagcggccg agcctgcatc gaccgaaacc gacgcgaccg
     7501 cctcgccccg ggtcgaggtg ccgcgtacgc cgaatcctcc cccagccgca ccggcctcgg
     7561 ctgccggacc gcgatcgagc gttgcgttcc aagccggccc gcaccgtgcc gcgcgccccc
     7621 agcccgcgga cgcttcgacg cccgcagccg ttcgcattgc gcctgcggcg ccggatccgg
     7681 ctcgcggcgc ccgcatctcc acgctcgaat gggacgcgct cgaagccgag atccgcgatt
     7741 gcaaggcctg tgggctgtgc gagcgccgca agcaggccgt gcccggcgtg ggcgaccgcc
     7801 aggcacgctg gatgctggtg ggcgaggcgc cgggcgcgga ggaggaccag cgtggcgagc
     7861 ccttcgtcgg ccaggccggg cgcctgctcg acaacatgct cgcggcgatc gggctgaagc
     7921 gcggagaaga cgtctatatc gcaaatgcgg tcaagtgccg accgccacac aaccgcactc
     7981 cagaacgcgg cgagatcgcc gcctgccagc cgtatctcga tcgccagatc gcgctcgtgc
     8041 agccgcagct gctggtcgcg ctcggccgtc cggccgccca ggccctgctc gatcgcgaga
     8101 tcgcaatctc ggccgcgcgc ggcaagcgct tcgagcgcgc gggcactccg gtcgtggtca
     8161 cctaccatcc ggcctatctg ctgcgcaatc cgcaggacaa ggccaaggca tgggaagacc
     8221 tctgcttcgc gcgccggctg atcgccgaga cggggtgaag gcggtggcgg ggtgccgggc
     8281 gggccggggg cgaaggccgc ccggcctcag tgcgggcgat cctcgagcag cgccacgttg
     8341 tcgaactcgc gctggttggc ctcgtggcgg cgcttcacca gcagcatggt gccggcgaca
     8401 aagagcccga acagcacgat cacggtgttg atcgacaggc cgagcacgat cagcagcgca
     8461 taggtgccgg tcatcatcag gatcgacagg ttctcgttga agttctgcac cgcgatcgag
     8521 tgcccggcac ccatcaggat gtggccgcgg tgctgcagca gggcgttcat cggcaccacg
     8581 aagaagcctg ccagcccgcc cacgatcacc atcagcgcca tcgcgaccca ggcctcggtg
     8641 acgccgacca tgatcatcac tccgaggccc atggcgatgc cgagcgggat cacgttcacc
     8701 gcgcgccgca gcgtgatgta gcgcgccgcc agcaccgcgc cggtggccac gccgagcgcc
     8761 accacgccct gcagcatcga ggcctgcgac aggttcatgc ccaggttgtg ctcggcccac
     8821 ttgatcacga tgaactgcag cgtcgcgcct gcgccccaga acagcgtggt gaccgcgagc
     8881 gagatctgcc cgagcttgtc gcgccagagc aggcgcaggc agtggttgaa gtcgtgcagc
     8941 aggtagagcg gattgctctt gagcggcttg tggtccaccc cggtgtcggg gatgtacatg
     9001 ttgaagatcg ccgccaccag gtacagggtg ccgaccacca gcaccgccgc gccgaaggcc
     9061 gagccggcgc cgggcaggcc cagccccatc agccaggtgg agacgtcgcc acggatcatg
     9121 atcccgccga tcacggtgcc gacgatgatc gccgccaccg tgaggccctc gatccagccg
     9181 ttggccacca ccagcaggcg gtggggcaga tactcggtga ggatgccgta cttcgccggc
     9241 gagtaggccg ccgcgcccag tccgatcacc gcgtaggcga agagtggatg cgtacccagg
     9301 aaaagcatca ggcagccgcc gatcttgatc gtgttgctga tcagcatcac tcgccacttg
     9361 ggcatcgaat cggcgaaggc gccgacgaag gcggcgagcg cgacgtagga gacggtgaag
     9421 aagagcttga gcagcggctc gtattcggag ggggccgcca tgtcccggag catggcgatg
     9481 gcgatgatca gcaaggcgtt gtcggccagc gcggagaaga actgcgctgc catgatgatg
     9541 tagaagccga gcggcatcgt cgggtcgcgg cggaaggtcg aagcgcggtt tatatcacga
     9601 agcggattgc cctgtgatga tctcgcgcag cgcttgtgcg agggcgcgca gatgcccgta
     9661 tggcaacggt ttgaggccgc agcgagcagg ttctcgggat gaacgcgcag ggatccacct
     9721 gaatgcgccg gtttgtgctt taattcacgt aaataaaacg tacttatcca cgccatggca
     9781 gatcgcagac tccaggtttt ccacgcggtt gcgaagcacg gctcgttcac ccgagccgcg
     9841 gaacacctcc acatgacgca accggccgtc accttccaga tcaagcagct cgaggaacac
     9901 ttcgacaccc gcctgctcga tcgcgggcac ggcaagatca cgctcaccgc cgcgggcgag
     9961 ctcgtgctcg cctacgccga gcgcatcctc ggtctgtcgg ccgagctcga ctcgcgcgtc
    10021 tccgaactca ccgacgagct cgccggccag ctgcgcatcg gcaccagcac gacgatcgcc
    10081 gcctactggc tgccgcaggt gctggagagc ttcaagcgcc gctaccccgg cgtgctgccg
    10141 cgcgtggtgg tcggaacctc caagctcacc gaggatcgcg tcgccgcgcg cgatctcgac
    10201 ctcggcctca tcgagatcgc caccgagcag cccagcatcg aacgccgcag cgccgcgcgc
    10261 gacgacctcc tggtgatctg cagccccggc catccgctgg cgaagttcaa ggagctcact
    10321 gcccgcgacc tcgtcggcca tcccttcatc gaccgcgacc ctggcaacgg catccgccag
    10381 atcgcagacg agttcttcga ggccgccggc atcgccggca gccagatcac cctgtgcgcc
    10441 gaactcggca gcctcgccgc ggtcaagcag ctcgcggccg cgggcctcgg cttcgcgatc
    10501 gcctccgggc gcgcgatccg gcgcgacgtg gaagagggca ggctggtggc gatcccgctg
    10561 cagccgcggc tgcacacccc gctcgaggtc atcctgccac gcgacaagtt ccgctcgcgc
    10621 ctgatcaacg ccttcgccga ccacgtctgc gaggagttcg cccgcatcgc gaccgagcag
    10681 gactgattcc ccgcaagctt ttgctccggc cgcggccgga gccctgtcgc agttacggaa
    10741 gaccctgatg gcacggcaga aatccgcttt cgtctgcacc gaatgcggtg cgcaggccgt
    10801 gcgctggcaa ggccagtgcc cgcagtgcca ggcctggaac acccttgtcg agaccgtgct
    10861 cgagcccgca gcgccggctg cgggcagccg cttcgccgcg ctcgccggcg agaccagccg
    10921 gctgcagccc ctgtccgcgc tcgaaccccg cgaagaaccc cgccagccca ccggtctcga
    10981 cgagttcgac cgcgtgctcg gtggcggcct ggtcgccggc ggcgtggtgc tgatcggcgg
    11041 cgacccgggt atcggaaaat ccaccctgct gctgcaggcg ctctccaacc tggccgcgac
    11101 gagtgcgtcg gctggcagcg agggcgcgac gggcaagggc accaaggcgg gcggcgtcat
    11161 ctacgtcagc ggcgaggagt cgggcgagca ggtcgcgctg cgtgcccagc gcctgcagtt
    11221 accgcagtcg cccctgcaga tgctcgccga gatcaacctc gaacgcatcc tccacaccct
    11281 gcgcgaggcg cgcccgcgcg tggcggtcat cgactcgatc cagaccgtct attccgagac
    11341 cctgcagtcc gcccccggct cggtcgccca agtgcgcgaa tgcgccgccc agctcacccg
    11401 ctttgccaag cagagcggca ccagcctgat cctggtcggc cacgtcacca aggacggcac
    11461 gctcgccggc ccgcgcgtgc tcgagcacat cgtagacacc gtgctgtact tcgagggcga
    11521 cacgcactcc agcttccgcc tgatccgcgc cttcaagaac cgcttcggcg cggtcaacga
    11581 gctcggcgtg ttcgccatga ccgagcgcgg cctgcgcggt gtgtccaatc cctccgcgat
    11641 cttcctgtcg cagcactcgc agcaggtcgc cggcagctgc gtgctgatca cccaggaggg
    11701 cacgcgcccg ctgctggtcg aggtgcaggc cttggtcgat tcgtcgcaga gccccaaccc
    11761 gcgccggctg tcggtgggcc tggagcagaa ccgcctggcg atgctgctcg cggtcatgca
    11821 ccgtcacgcc ggcgtggtct gcttcgacca ggacgttttc gtgaatgcgg tcggcggcgt
    11881 caagatcacc gagccggccg ccgacctcgc gatcctgttc gcgatcgtct cctcgctgcg
    11941 cgaccgcccg cttcggcgcg gcctcgcggt gttcggcgag gtcggtctcg ccggcgagat
    12001 ccggcccgca ccgcgtggcc aggagcgcct gaaggaagcc gccaagctcg gcttcgacac
    12061 cgccatcgtt ccgcgcgcca atgcgccgaa gcatccgatc gagggcctga aggtgatcgc
    12121 ggtcgagcgc gtggaggagg ccctggagca gttgcgcggg ttgtaagcgg gaaggtaagt
    12181 cgcatccctg tacgagcgcc gccaggcgcg aatgcagcgt cgaccgtccc acccacgtcg
    12241 attccccgca cgcaggaatc gcggctcgcg ccgctcccac acgacacccg caagcgcgcc
    12301 gtttcttgca ggagcgccgc caggcgcgaa tgcggcgtcg gccgtcccac ccacgtcgat
    12361 aatgagtccg ccgagttcgc ggcttgcgcc gcttctacag gtcggggagc caggccgtga
    12421 aggccgcgat ggcggcgcgc aggttggcgt tggcggaggg ggctggcgcg gtgccgagct
    12481 cccatgagtc accgcggatg gcgagcaacc aactgggcgg cgcgggccgg ccctcgaggt
    12541 cctcgaacac ctgtagcacg ctctgcggcg agaccgagtg ggtggtgaag ctgtcgtcgc
    12601 gggccgctgc gatcggctgg aattcgaaag gcggccgtgc cgtaccggat gcatcgacga
    12661 acaggatccg gctacggcga cgcagatcca gcgcatgctc gacctggagc tggaaatcgg
    12721 tcaggcactc gatccccggc agcttcatcg cctcgaccgc ctccacgaac agcggcccga
    12781 gcgcgtcgtc gccgcggctc ggatttcccc atccgaagac cagcaccggc gcgctcatgc
    12841 cttgtatttc cggtccagct ccgtgccctc ggcatccacc agcgtgacct ccagcggcat
    12901 ctgcccgagc gcgtgggtgg cgcaggacag gcaggggtcg aaggcgcgga tggcgacctc
    12961 gatgtggttg agcagtccct cggtgatctc ctggccgtgg aggtattgct tcgcgacctc
    13021 gcgcaccgcc gtgttcatcg cctggttgtt gttggtggtg gagacgatca ggttggccat
    13081 ggtgacgagg tcgtcgtcgc ccacctggta gtggtggaag agggtgccgc gcggggcctc
    13141 gatcacgccc acaccctcgc gccggcgctc gccgctcgtc atgagctcgg tgccggagag
    13201 gtcgtcgtcg tgaagcaggt ccttgatggt ctcggcggcg aagagcatct cgatcatgcg
    13261 cgcccagtgg aaggccaggc tgccgtgcag cggcttgccg ccggcgtagg cgacgagttc
    13321 gcggcgctcg tgctcggcaa acggcgtggg gatgctgtcg cagttctgca cgcgcgcgag
    13381 cgggccgacc ttgtaccagc ccgcctccgg gcccatcgac gtgaagtagg gaaacttcat
    13441 gtagctccag ggcttgacct cctcggtgat gaggcggtcg taagtctggt agtccacctg
    13501 gtcgaagagg atcctgccgt cggcgtccct ggcgcgcagc acgccgtggt agaggtcgag
    13561 gctgccgtcg ggcgcgacca ggcccatgaa gctcgagcgc acggtgccga agtggtcgta
    13621 gagcgcgcgg tcctgctggt ggagttcctt gatgatgcgc accgcgtcgc ggctccaggc
    13681 gatcatctgg taggcatcgg cgagcaggag cttgcgttcg tcggcgctca gcgcgcgatt
    13741 gacgcccccc ggtaccgcgc cggtgccatg cacgcgcttg ccggcggtga ggcggatcac
    13801 ctcctggccg aacttgcgca gcaggatgcc tttcttcgcg atgtcggggt attgctgtgc
    13861 cacgccgacg atgttgcgcc gacctagctc ggagccgaag ccgaacagca ggtcgggcga
    13921 ggacaggtgg aagaagtgca gcgcgtgcga ctgcaggatc tggccgtagt gcatcaggcg
    13981 gcgcatcttg tcggcggttg gcgtgacgcg tgcggcgccg acgacgaggt cgagcgcctt
    14041 gctcgcggcg aggtggtgcg acaccgggca gatgccgcac aggcgctgca ccatcaccgg
    14101 cacttcccag tacggccgcc cctggatgaa gcgctcgaag ccgcggaact ccacgatgtg
    14161 caggcgcacc tggtgcacct tgttgtcctc gtcgagcagg atggtgacct tgccgtggcc
    14221 ttccacgcgc gagacggggt cgattgcgat acggcgcaag gtatcgctgg cgactgtggc
    14281 ggtttcgagt tgaaagctca tttggatatc gtcgtcagtg agaggctcgc ggcttgcgcc
    14341 actcctgccg aaaagcgagg tgctcgagct tcctgcagga gcggcgcgag ccgcgagcct
    14401 ttcggggttt cagtcatagt gcagcagccc atgtcccagg ctcgggtcct tgccttcgat
    14461 caactgggtc aggaaggccc agatcgcgtc gcccgatggc gggcagccag gcaagaagta
    14521 atcgatcctc accacctcat gcaccggatg gacctggttg agcggcagcg gcagctcggg
    14581 gtcgtcggga atgcggctgc cctcggcgag accgtggccg gttcgataga cctcctgcag
    14641 gaggtcggcg agctcgaggt gattgcgctg tgcaggcagc ccgccgttga ccgcgcacgc
    14701 gccgagcgcg atcagcacct tgcagttggc gcggaattcg c
//
LOCUS       DAOQBS010000075        14652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3180_length_14652_cov_14.985134, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000075 DAOQBS010000000
VERSION     DAOQBS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 14652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14652
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3180_length_14652_cov_14.985134"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="PKN25_09680"
     CDS             complement(<1..159)
                     /locus_tag="PKN25_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357062.1"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNV91211.1"
                     /translation="MLQLAPYAVTETASRGRVHDEPAPVARGQFQRDRDRIVHSTAFR
                     RLEYKTQVF"
     gene            complement(290..1372)
                     /gene="aroB"
                     /locus_tag="PKN25_09685"
     CDS             complement(290..1372)
                     /gene="aroB"
                     /locus_tag="PKN25_09685"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNV91212.1"
                     /translation="MQTLNVALGERAYPIHIGRGLLGRADLILPHLKTQRVAIVTNEV
                     VGPLYLERLRAGLEDAGVRTAAVVLPDGEAHKDWETLARIFDMLLAERCERSTTLVAL
                     GGGVVGDMGGFAAACYQRGMPFIQVPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     RLVLADIDTLDTLPDRELSAGLAEVIKYGLIRDPGFLAWLELNLDRLMARDTDALAWA
                     IERSCRNKAEVVAADETEQGERALLNLGHTFGHAIETGLGYGEWLHGEAVAAGTMMAA
                     ELSRRLGWIDAAAVARIEALLERARLPVWGPRLGVERYVELMSHDKKVEAGKLRLVLL
                     RALGCAVMHGEAPAGEIAAAIEARCR"
     gene            complement(1360..1890)
                     /locus_tag="PKN25_09690"
     CDS             complement(1360..1890)
                     /locus_tag="PKN25_09690"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNV91213.1"
                     /translation="MSCLILIGMMGAGKTTVGKELARRRRMRFADCDQAIVARTGVSI
                     PTIFELEGEAGFRRRETQMIDELTREPDLVLATGGGVVVTPLNRELMRERGIVIYLDV
                     PTQVLFERTRHDRNRPLLQVENPRQRIEELHRARDPLYRETAHIILAGGRGNPCNMVR
                     QIEEALARYKADTCRP"
     gene            complement(1979..4099)
                     /locus_tag="PKN25_09695"
     CDS             complement(1979..4099)
                     /locus_tag="PKN25_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNV91214.1"
                     /translation="MRHRIGVLLTAAAFAGLAPAPAVLAQDASQTQSFDNRIEALEVA
                     EQGGAIYVRLTLREPLASPPPSFSVANPARIAFDFPGTANALGRNVQTIDQGDLRSAN
                     IVQAGDRTRLVLNLTRMSPYEARVQGRDLIIALSPAAARAVQGTAVSEAAANFAAPRS
                     NAAAAGGLAVRDINFRRGTGGEGRVVVDLSNPEAGIDIRQQGGNLVVDFLQAALPDHL
                     RRRSDVTDFATPVTSMTAQQVGDRVRLTVTPNGLWEHNAYQSDNRFVLEVRRVAEDPT
                     KLGGGRSDYKGDKLSLNFQNVDVRSVLQVIADFTDFNIITSDSVQGNLTLRLKDVPWD
                     QALDIILQAKGLDMRKNGNVIWIAPGDELAAREKLQLEAKAQIVDLEPLQTESFQINY
                     HKAKEIFDFLKSKDQTMLSKRGSVVVDERSNKVFVTDVATRLQALRRLVQEIDVAPRQ
                     VLIEARIVEANKTFARDLGVRLGFGSKGVASLDGARLGFGSSSLTGGGSNNPNTFTPT
                     VAQSGTGFMPVGYGSLGLSLFNSRATRFLNLELQALETDGRGRVVSSPRVLTANQVEA
                     SIEQGTEIPYQEASSSGATSVSFKKAVLSLKVKPQITPDGRLQLSIEVNKDRPLYEQT
                     LLGVPPIETKNVKSEVLIENGGTVVIGGIYEEEESTGEDKVPVLGDVPVLGHLFKSQS
                     KVSNRKELLVFITPRIVSDALTLR"
     gene            complement(4096..4620)
                     /locus_tag="PKN25_09700"
     CDS             complement(4096..4620)
                     /locus_tag="PKN25_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HNV91215.1"
                     /translation="MMNRAVLIGACVLLAGCAGGGQDDLEAWMGEQAKTMRGAVRPLP
                     ELRQVAVVAYAGTQGDDPFRSARMEPERRASASALRPDLDRRREPLEAFPLESLQMVG
                     VLMQGNNTHALVKAGTVLHQVKAGNYMGQNFGVITLVTENEIKLRELVEDVYGDWVER
                     TSTLTLQERQEAGK"
     gene            complement(4617..5291)
                     /locus_tag="PKN25_09705"
     CDS             complement(4617..5291)
                     /locus_tag="PKN25_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNV91216.1"
                     /translation="MSFDLGALRDGIDFRRLAEDFRGLDPQDPGMWPAAPRVVVAVCL
                     LLLTVAGFWWFDWQGQVETLEARQTEEVQLRESWVTKKRQAVNLEEHRRQLAEIDRQF
                     GALLKQLPNRAEMDSLLSDINQAGLGRGLQFELFKPGADVIKEFYAEMPIDVRVTGVY
                     HDLGEFVADLASMPRVVTLGDVGIEADKDGRLKLQAKAVTYRYLDEEELAQQRKAAQA
                     AKQGNK"
     gene            complement(5288..5893)
                     /locus_tag="PKN25_09710"
     CDS             complement(5288..5893)
                     /locus_tag="PKN25_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNV91217.1"
                     /translation="MIRINLLPHRIAKRRQRRRQFYALAGSMVLLGALIGFGVHMVYT
                     AHIEDQEARNAFLKEEIVRLDREIAEIRRLREQIDAMLARKQVIESLQGTRAETVHLF
                     NELAQRLPDGVYLKSVKQTGRQVRVVGLAQSNARVSHFMRNVEASAFLEQPRLIEAKA
                     VTQDNRRLSEFSLDIAIRPPVQAEPEGAAGAAKAAAKGTQK"
     gene            complement(5890..6966)
                     /locus_tag="PKN25_09715"
     CDS             complement(5890..6966)
                     /locus_tag="PKN25_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNV91218.1"
                     /translation="MIDLPFFSPSARQLVGLDISSSSVKLVELSAGEKDAYKVERYAV
                     ETLPRDAVVDGNIANLDAVSESVRRALRRFGSGVRNVALALPSSSVITKKIILPEGLR
                     ETEMEFAVEAEANQYIPFALEEVNLDFQRIGPAPGSPGDVEVLIAASRKDKVEDRVAV
                     AQAAGLKAVIVDVESLATESALELVCRQLPRGGADQVIALVDIGASVMNVTMFRNGHS
                     IYSREQAFGGSQLTQDIARQYGMSFEEAESAKRAGSLPDSYPRDLMRPFMDSLALEVS
                     RALQFFFTSTPFNQVDHVVLAGGCAVLPGLGDVVAARTQVDTLIANPFASMTLSGKVR
                     PKALLADAPALMVACGLALRRFDA"
     gene            7154..9577
                     /locus_tag="PKN25_09720"
     CDS             7154..9577
                     /locus_tag="PKN25_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNV91219.1"
                     /translation="MRWVLYPIAALLALAILGIATLAAMAFFAWPNLPALDTLTDYRP
                     RVPLRVYTADGHLIGEFGEERRDVVRIAEVPSHLKHAILAAEDERFYEHRGIDPLGLA
                     RAALANLSSGGRGQGASTITMQVARNFFLSREKTYNRKLYEILLSLKIEQNLGKDQIL
                     ELYINQIFLGQRAYGFAAAARAYYGKTLEQLTLPEAAMLAGLPKAPSAFNPIVNPARA
                     AVRQQYVLRRMLEAGFIDDAQYQHALAFATPKQSQRDIAAARRVQDIVVPGDYVAEMA
                     RQIAVEQFGEQAYELGIRIITTVTRKDQEAAYAALRRGVLDYDRRHGYRGPEAFVELP
                     SGQVPDEQLDEALGETNDHDDLLAALVLRASPKEVLAYRRGQEIRITGKGLSFVAPML
                     SDKAPQARRVRRGAIVRVRNTGKDGWELAQLPEVQAALVAIDSHSGAVRALIGGFDFH
                     RNKFNHVTQAVRQPGSSFKPFIFSAALDRQFGPGSVLEDNPLYYPAGVTGSKAWEPKN
                     YDGRYAGPMTLRDGLARSKNMISIRLLEKITPDYARDYLARFGFDPAQNPPYLTMALG
                     AGAATPWQMAAGYAVFANGGYRVDPFVVKEMYDGSGRLIARTDPPVAGQSAPRVIDAR
                     NAWLMDSMMRDVVQRGTATRARSLKREDIAGKTGTTNDYVDAWFCGYNPDLVAVSWVG
                     HSQPRNLGKGETGGAAALPIWINYMASALEGAPQVELPRPEGLMQVQNGLGTSQDWQY
                     AEYAPPAPPPVPDWLRDMFANDSAPAMEAESEAIPPGPAPLILPPPSPARAQAPAPVE
                     ERRPMPIRP"
     gene            complement(9712..10038)
                     /gene="cyaY"
                     /locus_tag="PKN25_09725"
     CDS             complement(9712..10038)
                     /gene="cyaY"
                     /locus_tag="PKN25_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HNV91220.1"
                     /translation="MEESVFNALAEAELARIETALEDCGAELDVEPKPGGILEIEFDN
                     GSKMIINRHTAAREIWVAAKSGGFHFRPLDGGWVNTRDGRELWAMLAALVGEQSGEAV
                     RLLRPS"
     assembly_gap    10119..10218
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10338..11609
                     /gene="lysA"
                     /locus_tag="PKN25_09730"
     CDS             10338..11609
                     /gene="lysA"
                     /locus_tag="PKN25_09730"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNV91221.1"
                     /translation="MNAPASRWPAPTLSARDGALWLEELPLAEIAARFGTPTYVYSRT
                     ALEAAFAAWKDALAGRRALVCYAVKANSNLGILSVFARLGAGFDIVSGGELARVLAAG
                     GRADKVVFSGVGKTRAEMRQALQAGIRCFNVESAAELEHLDAVAGELGVRAPVSLRVN
                     PDVDPKTHPYISTGLKGNKFGVAFADAPALYRRAAALPNLRITGVACHIGSQLLDRAP
                     IAEAAQKLRGLVEGLAADGIVLEHIDLGGGLGIRYRDETPPPVAEYLAPLLEVFADRE
                     EELCFEPGRSLVGNAGLLLTRIEYLKPGETKNFAIVDAAMNDLARPALYDAWHEVVEV
                     TPRALAPTTYDVVGPICESGDFLARARSLAVAEGDLLAILSAGAYGMTMASNYNTRPR
                     AAEILVDGASAHVVRERETVEELFARERTPG"
     gene            complement(11704..12054)
                     /locus_tag="PKN25_09735"
     CDS             complement(11704..12054)
                     /locus_tag="PKN25_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91222.1"
                     /translation="MSGFGHYTRTADELEREIVKRGIAIGIDWDDASRMRELAHRALT
                     CTPACMMKLLRSPVRQDKLTGELFALSELMLQNMRQSAEIGFETHGGPAWKAFGRALN
                     EEYDAGARPPVASA"
     gene            12198..13631
                     /gene="dacB"
                     /locus_tag="PKN25_09740"
     CDS             12198..13631
                     /gene="dacB"
                     /locus_tag="PKN25_09740"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNV91223.1"
                     /translation="MKNPSLCALFIAGLLLAPITHAGVAGLPPGVRLALDSARIPAEA
                     VSLWTRPVDHATPALAINATAPMNPASVMKLVTAFSALERLGPAHTWTTRIARDGPIR
                     AGVLEGNLYLVGGADPVLGYDRLWKVLRRLRALGIERVAGDIVLDASVLRLPPHDPDA
                     FDGRGVRPYNSGPYGLLLQYNTLLLGLTPGKAANAPVALAAEPPLAGLVIDNRVLTSD
                     APCGSWYQDLQAVVEPGRRLVLSGSLPAACGPRTWSAAPLPPAEFGSALVAALWSEVG
                     GHLGGTVREGRLPTGAEELFSDDSDPLADVVRDMNKWSSNVIARQLLATLGRVSLRED
                     ATEDMVTAGARAAAATLAAAGIDTDGLVIENGSGLSRIERIRADSLGMLLLAAWQRPW
                     MPEFIAALPVAGVDGTARRRLGDSPARGQAHIKTGTLNGVRAMAGYLLDRNGQRHAVV
                     MMVNHPEAAASAAAQDALLEWLWSGGE"
     gene            complement(13805..14218)
                     /locus_tag="PKN25_09745"
     CDS             complement(13805..14218)
                     /locus_tag="PKN25_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91224.1"
                     /translation="MNSPYPAPLEAGQPSENMVTVTHLVYALHAFAVFSAVVGSATII
                     FSFVASLPSIAAVILNYWNQNAVRGTWLESHFRWQIRTFWFAVLWIAVSVLMVVTIIG
                     VPFALALLLIAGLWILYRVVRGWWNLMQRQTMPMA"
     gene            complement(14330..>14652)
                     /locus_tag="PKN25_09750"
     CDS             complement(14330..>14652)
                     /locus_tag="PKN25_09750"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004886738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNV91225.1"
                     /translation="IWSFLRQFMYTKFIIVVDEDVNIRDWKEVIWALTTRMDATRDTT
                     LVDNTPIDYLDFASPVAGLGSKMGLDATNKWPGETSREWGTPIVMDAAVKAKVDAMWG
                     ELGL"
BASE COUNT         2262 a         5210 c         4838 g         2242 t
ORIGIN      
        1 gaacacctgg gtcttgtatt ccaggcggcg gaaggcggtg gaatgcacga tgcggtcgcg
       61 gtcgcgctgg aactggccgc gcgcgaccgg ggcaggctcg tcatgaacac ggccgcgcga
      121 cgcggtctcg gtgacggcat agggggcgag ttgcagcatg caggctcctg aattggcgca
      181 gcaagacgac agcgggcgga cgttcctcgc cgccccattc gcgggcatgc ccgcgcccac
      241 gacggcggag cacgcagcgg gagcgccctc gaccgcgctc gccgcgaact cagcggcagc
      301 gcgcctcgat cgccgcggcg atctcgccgg ccggggcctc gccgtgcatc accgcgcacc
      361 cgagcgcgcg cagcagcacc aggcgcagct tgccagcctc gaccttcttg tcgtgcgaca
      421 tcagctcgac atagcgctcg acgccgagcc gcgggcccca caccggcagg cgggcgcgct
      481 cgagcagcgc ctcgatgcgc gccaccgcgg cggcgtcgat ccagcccagg cggcgcgaca
      541 gctcggcggc catcatcgtg ccggccgcga ccgcctcgcc gtgcagccat tcgccgtagc
      601 cgaggcctgt ttcgatggcg tgaccgaagg tgtgaccgag attgagcagc gcgcgctcgc
      661 cctgctcggt ctcgtcggct gccaccacct cggccttgtt gcggcaggag cgctcgatcg
      721 cccacgcgag cgcatcggtg tcgcgcgcca tcaggcggtc cagattgagc tccagccagg
      781 cgaggaagcc ggggtcgcgg atcaggccgt acttgatcac ctcggccagg ccggcggaga
      841 gctcgcgatc gggcagggtg tcgagggtgt cgatgtcggc gagcaccagc ctgggctggt
      901 agaaggcgcc gatcatgttc ttgccgagcg ggtggttgat cgcggtcttg ccgcccaccg
      961 aggagtcgac ctgcgacagc agcgtggtcg gcacctggat gaagggcatg ccgcgctggt
     1021 agcaggccgc ggcgaagccg cccatgtcgc ccaccacccc gccgccgagc gcgaccaggg
     1081 tggtggagcg ctcgcagcgc tcggcgagca gcatgtcgaa gatgcgggcg agcgtctccc
     1141 agtccttgtg cgcctcgcca tcgggcagca ccaccgcggc ggtgcgcacg cccgcgtcct
     1201 cgaggccggc gcgcaggcgt tcgaggtaga gcgggcccac cacctcgttg gtgacgatgg
     1261 cgacgcgctg cgtcttcagg tgaggcagga tcaggtcggc gcgcccgagc aggccgcggc
     1321 cgatgtggat gggataggcg cgctcgccga gcgccacgtt cagggtctgc atgtgtcggc
     1381 cttgtagcgg gcgagcgcct cctcgatctg gcgcaccatg ttgcaggggt tgccgcggcc
     1441 gcctgcgagg atgatgtggg cggtctcgcg atagaggggg tcgcgcgcgc ggtggagttc
     1501 ctcgatgcgc tggcgcggat tttcgacctg cagcagcggg cggttgcggt cgtgacgggt
     1561 gcgctcgaac agcacctgcg tcggcacgtc gaggtagatc acgatgccgc gctcgcgcat
     1621 cagctcgcgg ttgagcggcg tgacgaccac cccgccgccg gtggcgagca cgaggtcggg
     1681 ctcgcgggtg agctcgtcga tcatctgcgt ctcgcgacgg cggaatcccg cctcgccctc
     1741 gagctcgaag atggtcggaa tgctgacgcc ggtgcgcgcc acgatcgcct ggtcgcaatc
     1801 ggcgaagcgc atccggcgcc tgcgtgccag ttccttgccg accgtggtct tgccggcgcc
     1861 catcatgccg atcagtatca gacagctcac gcgtgactcg ccggaaatcc tgatgaaaaa
     1921 gggcgggcat ccatgccgga tgcccgccct cgggattgta tcagcagaag cgccggactc
     1981 agcgcagcgt gagcgcgtcg gagacgatgc gcggggtgat gaagacgagg agctccttgc
     2041 ggttcgacac cttgctctgc gacttgaaca gatggccgag caccggcacg tcgccgagca
     2101 ccggcacctt gtcctcgccg gtcgactcct cttcctcgta gatgccaccg atcacgacgg
     2161 tcccgccgtt ctcgatcagc acctcgctct tgacgttctt ggtctcgatc ggcggcacgc
     2221 cgagcagggt ctgctcgtag agcgggcggt ccttgttgac ctcgatcgac agctgcaggc
     2281 ggccgtcggg ggtgatctgc ggcttgacct tgagcgacag caccgccttc ttgaaggaca
     2341 cgctggtcgc gccggagctc gacgcctcct ggtaaggaat ctcggtgccc tgctcgatcg
     2401 aagcctcgac ctggttggcg gtgagaacgc gcgggctgga aaccacccgc ccacggccat
     2461 cggtctcgag agcctgcagc tcgaggttga ggaaacgggt ggctcggctg ttgaacaggc
     2521 tgagcccgag tgagccgtag ccgaccggca tgaaccccgt ccccgactgc gcgacggtcg
     2581 gcgtgaaggt gttgggattg ttgctccccc cccctgtcag cgacgacgaa ccaaagccga
     2641 ggcgtgcacc atccagcgag gcgacgccct tgctgccgaa accgagccgg acccccagat
     2701 cgcgcgcgaa ggtcttgttg gcctcgacga tgcgcgcctc gatcagcacc tggcgcggcg
     2761 ccacgtcgat ctcctgcacc agccggcgca aggcctgcaa gcgggtggcg acgtcggtca
     2821 cgaagacctt gttgctgcgc tcgtccacca ccacgctgcc acgcttcgag agcatggtct
     2881 ggtccttgct cttgaggaaa tcgaagatct ccttggcctt gtggtagttg atctggaagc
     2941 tctcggtctg cagcggctcg aggtcgacga tctgcgcctt ggcctcgagc tgcagcttct
     3001 cgcgcgcggc gagttcgtca cccggggcga tccagatgac gttgccgttc ttgcgcatgt
     3061 ccaggccctt ggcctgcagg atgatgtcga gcgcctggtc ccagggcacg tccttcaggc
     3121 gcagggtcag gttgccctgc accgagtcgg acgtgatgat attgaagtcg gtgaagtcgg
     3181 cgatgacctg cagcaccgag cgcacatcca cattctggaa gttcagcgag agcttgtcgc
     3241 ccttgtagtc gctgcgcccg ccgccgagct tggtcgggtc ctcggcgacg cgccgcacct
     3301 cgagcacgaa gcggttgtcg ctctggtagg cgttgtgctc ccacagccca ttcggagtca
     3361 cggtcaggcg tacgcggtcg cccacctgct gggccgtcat cgaggtgacc ggggtggcga
     3421 agtcggtgac gtcggagcgg cggcgcaggt ggtcgggcaa cgcggcctgc aggaagtcga
     3481 cgaccaggtt gccgccttgc tggcggatgt cgatgccggc ctccggattc gacaggtcga
     3541 ccaccacgcg cccctcgccg ccggtgccgc ggcggaagtt gatgtcgcgc accgcgaggc
     3601 cgcccgcggc cgccgcattc gaacgcggcg cggcgaagtt ggccgcagct tcgctcaccg
     3661 ccgtgccctg caccgcgcgc gcagcggccg gcgagagcgc gatgatgagg tcgcgaccct
     3721 gcacgcgcgc ctcgtagggg ctcatgcggg tgaggttgag cacgagccgg gtgcggtcgc
     3781 cggcctggac gatgttggcg ctgcgcaggt cgccctggtc gatggtctgc acgttgcgcc
     3841 cgagggcgtt cgcggtaccc ggaaaatcga aggcgatgcg cgccgggttg gccacgctga
     3901 agctcggcgg cgggctggcg agcggctcgc gcagggtcag gcgcacgtag atcgcgccgc
     3961 cctgttcggc gacctccagc gcctcgatcc ggttgtcgaa gctctgcgtc tgcgacgcgt
     4021 cctgggccag cacggcaggc gcgggcgcca ggcctgcgaa agcggctgcg gtcagcagca
     4081 ctccgatgcg gtgtctcatt ttcctgcctc ctggcgctcc tgcaatgtca gcgtgctggt
     4141 ccgttcaacc cagtcgccat agacatcctc gaccagttcg cgcagcttga tctcgttctc
     4201 ggtgaccaga gtgatcacgc cgaaattctg tcccatgtag ttcccggcct tcacctgatg
     4261 gagcacggtc cccgccttga ccagcgcatg ggtgttgttt ccctgcatca gcacgcccac
     4321 catctgcagc gattcgagcg ggaaggcctc gagcggctcg cggcggcgat cgaggtcggg
     4381 gcgcagcgcg ctcgccgagg cccgccgctc gggctccatg cgcgcactgc ggaagggatc
     4441 gtctccctgc gttcccgcat aggcgacgac cgcgacctgg cgcaattcgg gcaggggccg
     4501 caccgcgccg cgcatggtct tggcctgctc gcccatccag gcctcgaggt cgtcctggcc
     4561 acccccggca cagccggcga gcagtacaca ggcgccgatc aacacggccc tgttcatcat
     4621 ttgttcccct gcttcgcggc ctgcgccgcc ttgcgttgct gcgcgagctc ttcctcgtcg
     4681 aggtagcggt aggtgaccgc cttggcctgc agcttgaggc gtccatcctt gtcggcctcg
     4741 atgccgacgt cgcccagggt caccactcgc ggcatgctgg cgaggtcggc gacgaactcc
     4801 ccgagatcgt gatagacgcc ggtcacgcgc acgtcgatcg gcatctcggc gtagaactcc
     4861 ttgatcacgt ccgcgcccgg cttgaagagc tcgaactgca ggccacgccc gaggccggcc
     4921 tggttgatgt ccgaaagcag cgagtccatc tcggcgcggt tgggcaactg cttgagcagg
     4981 gcgccgaact ggcgatcgat ctcggcgagc tggcggcggt gctcctcgag attgactgcc
     5041 tggcgcttct tcgtcaccca gctctcgcgc agctgcacct cctcggtctg gcgcgcctcc
     5101 agggtctcga cctggccctg ccagtcgaac caccagaacc cggcgaccgt aagcaacagc
     5161 agacagaccg cgaccaccac gcgcggcgcg gcgggccaca tgccgggatc ctgcggatca
     5221 aggccgcgga aatcctccgc caggcggcga aagtcgatgc cgtcgcgcag cgcgcccaga
     5281 tcgaagctca tttttgcgtc cccttcgcgg ccgccttcgc ggcgccggcg gccccctcgg
     5341 gctccgcctg caccggcggg cggatggcga tgtcgaggga gaactcgctc aagcggcggt
     5401 tatcctgggt gacggccttg gcctcgatca accggggctg ctccaggaag gcggaggcct
     5461 cgacgttgcg catgaagtgc gacacccgcg cgttcgactg cgccagcccg acgaccctca
     5521 cctgcctgcc ggtctgcttg accgatttca gatatacgcc gtcgggcagg cgctgggcga
     5581 gctcgttgaa cagatgcacg gtttcggccc gggtcccctg cagcgactcg atcacctgct
     5641 tgcgcgccag catcgcatcg atctgttcgc gcaggcggcg gatctccgcg atttcgcggt
     5701 cgagccggac gatctcctcc ttgaggaagg cgttgcgcgc ctcctggtcc tcgatgtgtg
     5761 cggtgtaaac catgtgcacg ccgaagccga tcagcgcccc cagcagcacc atgctgccgg
     5821 cgagcgcgta gaactgccgc ctgcgctgcc tgcgcttggc aatccggtgc ggcagcagat
     5881 tgatgcggat catgcgtcga acctccgcag cgccagcccg cacgcgacca tcagcgccgg
     5941 cgcgtcggcg agcagcgcct tcgggcggac cttgccgctc aaggtcatgc tcgcgaaggg
     6001 attggcgatg agcgtatcga cctgcgtacg cgccgcgacg acgtcgccga gtcccggcag
     6061 caccgcgcag ccgcccgcaa gcacgacgtg gtcgacctgg ttgaacggcg tggaggtgaa
     6121 gaagaactgc aaggcgcgcg agacctcgag cgcgaggctg tccatgaaag gacgcatcag
     6181 gtcgcgcggg tagctgtcgg gcagcgagcc ggcgcgcttg gcggattccg cttcctcgaa
     6241 gctcatgccg tactggcgtg cgatgtcctg ggtcaactgg ctgccgccga aggcctgctc
     6301 gcgcgaatag atggagtggc cgttgcggaa catcgtcacg ttcatgaccg aggcgccgat
     6361 gtcgaccagc gcgatcacct ggtcggctcc gccgcgcggc aactgccggc acaccaactc
     6421 cagggcggac tcggtggcca gcgactccac atcgacgatc accgccttga gcccggccgc
     6481 ctgcgccacc gcgacgcgat cctccacctt gtccttgcgc gaggcggcga tcaggacctc
     6541 gacatccccg ggactgccgg gtgcggggcc gatcctctgg aagtcgagat tgacctcctc
     6601 gagcgcgaaa gggatgtact gattggcctc ggcctcgacc gcgaactcca tctcggtctc
     6661 gcgcaggcct tcggggagaa tgatcttctt ggtgatgacc gaggacgacg gcaacgcgag
     6721 cgccacgttg cgcacgccgg agccgaagcg gcgcagcgcg cgccgcacgg attcgctcac
     6781 cgcgtcgagg ttggcgatgt tgccatcgac caccgcatcg cgcggcaggg tctcgaccgc
     6841 atagcgctcc actttgtaag cgtccttttc cccggccgat agctcgacga gcttcacgga
     6901 tgaagacgag atgtcgagcc cgacgagctg gcgcgccgaa gggctgaaaa aggggaggtc
     6961 gatcacaaag aaaacccttg aatttcttta tgttacgcgc tattgctcag caacgctcta
     7021 gcatgctagc aacaagccat tggggtgtaa agcgaacccc gtcacggtcg agaccggctc
     7081 atccacggac acacgagcca cgcgggcgca tcccgtataa tgccgctccg ctcgaaacca
     7141 tcctggattg cccatgcgct gggtcctgta tccgatcgca gcgctgctcg cgctggccat
     7201 tctcggcatc gccacgctcg cggcgatggc gttcttcgcc tggcccaacc tgcccgcgct
     7261 cgacacgctc accgactacc ggccgcgtgt tccgctccga gtatatacgg ccgatggcca
     7321 cctcatcggc gagttcggcg aggagcgccg tgacgtagtt cgcatcgcag aggttccctc
     7381 ccacctcaag cacgccatcc tcgccgccga ggacgagcgc ttctacgaac accgcggcat
     7441 cgacccgctc ggcctcgccc gcgccgcact cgccaacctg tcgtccgggg gccgcggcca
     7501 gggggcctcg acgatcacca tgcaggtcgc gcgcaatttc ttcctctccc gggagaagac
     7561 ctacaaccgc aagctctacg agatcctgct ctcgctcaag atcgagcaga acctcggcaa
     7621 ggaccagatc ctcgagctct acatcaacca gatcttcctc ggccagcgcg cctacggctt
     7681 cgccgccgct gcacgcgcct actacgggaa gacactcgaa cagctcaccc tgcccgaagc
     7741 ggcgatgctc gccggccttc ccaaggcccc ctcggccttc aacccgatcg tgaaccccgc
     7801 gcgcgccgca gtgcggcagc aatacgtcct gcgccgcatg ctcgaagccg gcttcatcga
     7861 cgacgcccag tatcagcatg cgctcgcctt cgccactccg aagcaaagcc agcgcgacat
     7921 cgccgcggcc cgccgcgtcc aggacatcgt cgtccccgga gactacgtcg ccgagatggc
     7981 gcgccagatc gcggtggaac agttcggcga gcaggcctac gagctcggca tccgcatcat
     8041 caccacggtc acccgcaagg accaggaagc ggcctacgcc gcgctgcgcc gcggcgtgct
     8101 cgactacgac cgccgccacg gctatcgcgg ccccgaggcc ttcgtcgagc tgccctccgg
     8161 gcaggtgccc gacgagcaac tcgacgaggc actcggcgag accaacgacc acgacgacct
     8221 gcttgccgca ctggtgctca gggccagccc gaaggaggtg ctcgcctacc gccgcggcca
     8281 ggagatccgc atcaccggca agggcctgtc cttcgtcgcg ccgatgctct cggacaaggc
     8341 cccgcaagcc cgacgcgtgc gccgcggggc gatcgtgcgc gtgcgcaata ccggcaagga
     8401 tggttgggag ctcgcacagc tgccggaagt gcaggccgcg ctggtggcga tcgactcgca
     8461 cagcggcgcg gtgcgcgcgc tgatcggcgg cttcgacttc caccgcaaca agttcaacca
     8521 tgtgacccag gcggtgcgcc aacccggatc gagcttcaag cccttcatct tctcggccgc
     8581 gctcgaccgc cagttcggtc cgggaagcgt gctcgaggac aacccgctct actacccggc
     8641 gggggtcacg ggcagcaagg cctgggagcc gaagaactac gacgggcgct atgcgggacc
     8701 gatgaccctg cgcgacggcc tggcgcgctc gaaaaacatg atctcgatcc ggctgctgga
     8761 gaagatcacc cccgactacg cacgcgacta cctcgcccgc ttcggcttcg accctgcgca
     8821 gaatcccccc tacctgacca tggcgctcgg cgccggcgcc gccaccccct ggcagatggc
     8881 ggcgggctac gcggtgttcg cgaacggcgg ctaccgggtc gatcccttcg tggtcaagga
     8941 aatgtacgac ggcagcggcc gcctgatcgc gcgtaccgac ccgccggtgg cgggccagag
     9001 cgcgccgcgc gtgatcgatg cgcgcaacgc ctggctgatg gactcgatga tgcgcgacgt
     9061 ggtccagcgc ggcaccgcga cacgcgcgcg cagcctcaag cgcgaggaca tcgccgggaa
     9121 gaccggaacc accaacgact acgtcgacgc ctggttctgc ggctacaacc cggacctcgt
     9181 cgcggtgagc tgggtcggcc acagccagcc gcgcaacctc ggcaagggcg agaccggcgg
     9241 cgcggccgcg ctgccgatct ggatcaacta catggcctcc gcgctcgaag gcgcgccgca
     9301 ggtcgaactg ccgcgccccg agggcctgat gcaggtgcag aacggtctgg gcaccagcca
     9361 ggactggcag tatgccgaat acgctccgcc cgcgccgccg ccggttccgg actggctgcg
     9421 cgacatgttc gccaacgaca gtgcaccggc gatggaggcc gaaagcgagg cgatcccgcc
     9481 cggcccggca ccgctgatcc tgccgccgcc cagcccggcg cgcgcacagg cgcccgcccc
     9541 ggtcgaggaa cgccggccga tgccgatccg gccttgagca ggcgggcgct cgcgggatgg
     9601 cgactcgcgt cgatcctgca aggggcggaa cccgcgatga cgactccgcg gccgccgtgc
     9661 aggaacgacg caagtcgcca tcgggcgccc cggaaggagc gccccttccg ctcagctcgg
     9721 ccgcagcagc ctcaccgctt cgccggactg ctcgcccacc agcgcggcga gcatcgccca
     9781 cagctcgcga ccatcgcggg tgttgaccca gcccccgtcc agcggacgga agtggaagcc
     9841 gcccgacttc gccgccaccc agatctcgcg cgccgcggtg tggcggttga tgatcatctt
     9901 gctgccgttg tcgaactcga tctcgaggat cccacccggc ttgggctcga cgtcgagctc
     9961 ggccccgcaa tcctccaggg ccgtctcgat gcgcgccagc tcggcctcgg cgagcgcatt
    10021 gaacacagac tcttccatgc tgattccttc tgttagcatt ccggtttttt gcgaagaacg
    10081 gcgatgcgag tcccacagcg agtcccgttg cgagtcccnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnntg cggcatcaag ggcccgctct atctgccggc gcccggcacc
    10261 gcctcggcgc ccccggcggg cgccgatcat agcaaacccg tcgtgccgaa ggatctgatc
    10321 aaggaatcgc cgctcgaatg aacgcccccg cctcccgctg gcccgccccc accctgtccg
    10381 cgcgcgacgg cgcgctctgg ctggaggagc tgccgctcgc cgagatcgcc gcgcgcttcg
    10441 gcacccctac ctatgtgtac tcgcgcaccg cgctcgaggc cgccttcgcg gcctggaagg
    10501 acgcgctcgc cgggcgccgg gcgctggtgt gctacgcggt caaggccaac tccaacctcg
    10561 gcatcctgtc ggtgttcgcg cgcctgggcg caggcttcga catcgtctcg ggcggcgagc
    10621 tcgcgcgcgt gctggccgcc ggcgggcgtg ccgacaaggt ggtgttctcg ggcgtcggca
    10681 agacccgcgc cgagatgcgc caggcgctgc aggcgggcat ccgctgcttc aacgtcgagt
    10741 cggccgcgga actcgagcac ctcgacgcgg tggcgggcga gctcggcgtg cgcgcgccgg
    10801 tgtcgctgcg cgtgaatccg gacgtcgacc ccaagaccca cccctacatc tccaccggcc
    10861 tcaagggcaa caagttcggc gtcgccttcg cggacgcccc ggcgctctac cgccgcgccg
    10921 cggcgctgcc caacctgcgc atcaccggcg tcgcctgcca catcggctcg cagctgctcg
    10981 accgcgcgcc catcgccgag gccgcacaga agctgcgcgg cctggtcgaa ggcctcgccg
    11041 ccgacggcat cgtgctggaa cacatcgacc tcggcggcgg cctgggcatc cgctatcgcg
    11101 acgagacccc accaccggtc gcggaatacc tcgccccgct gctggaggtc tttgcggatc
    11161 gcgaggaaga gctctgcttc gagcccggcc gctccctggt cggcaacgcc ggtctgctgc
    11221 tgacccgcat cgaatacctc aagccgggcg aaacgaagaa cttcgcgatc gtcgatgcgg
    11281 ccatgaacga cctcgcccgc cccgcgctct acgacgcctg gcacgaggtg gtcgaggtca
    11341 cgccgcgcgc cctcgccccc accacctacg acgtggtcgg gccgatctgc gagagcggcg
    11401 atttcctcgc ccgtgcgcgc agcctcgcgg tggccgaggg cgacctgctc gcgatcctgt
    11461 cggcaggcgc ctacggcatg accatggctt caaactacaa cacccgccca cgcgctgccg
    11521 agatcctggt cgacggcgcc agcgcgcacg tggtgcgcga gcgtgagacg gtggaagagc
    11581 tgttcgcgcg cgagcgcacg ccggggtaag acggcgggag cccggtggcg agggcaacgg
    11641 ccgcagatcc cgcgatcggc ccgcgatcgg accgacgcac ctgctcggcg tatggggccc
    11701 gcctcaggcg ctcgccaccg gcgggcgcgc cccggcgtcg tattcctcgt tgagcgcgcg
    11761 cccgaaggcc ttccaggccg gtccgccgtg cgtttcgaag ccgatctcgg cgctctggcg
    11821 catgttctgc agcatcagct cggagagcgc gaagagctcg ccggtgagct tgtcctggcg
    11881 caccgggctg cgcagcagct tcatcatgca ggcgggcgtg caagtcagcg cacggtgtgc
    11941 cagctcgcgc atcctcgacg cgtcgtccca gtcgatgccg atcgcgatgc cgcgcttgac
    12001 gatttcacgc tccagttcat ccgcggtgcg cgtgtagtgt ccgaatccgc tcatctttcc
    12061 cgtgtatgcc tgccgcgtga ctgcagatgc gcggcgggtg tgccgctccg aatggaaacc
    12121 gccgcgcagc tgcgccggcg aagggcgcat tctagcgcct cgcccgcctg cactcgatca
    12181 ggatccctgt gctgcccatg aagaatcctt ccctttgcgc gctattcatc gccggcctgc
    12241 tgctggcccc gatcacccac gccggcgtcg ccggcctgcc gcccggggtg cgccttgcac
    12301 tcgacagcgc gcgcatcccg gcagaggcgg tgagcctctg gacccgccct gtggaccacg
    12361 ccacgcccgc gctcgcaatc aacgccaccg caccgatgaa tccggcatcg gtgatgaagc
    12421 tcgtcaccgc cttcagcgcg ctcgaacgcc tcggccccgc ccacacctgg acgacccgca
    12481 tcgcccgcga cggcccgatc cgtgccgggg tgctggaagg caacctctac ctcgtcggcg
    12541 gcgccgatcc ggtactgggc tacgaccgcc tgtggaaggt gctgcgccgc ttgcgcgcgc
    12601 tcggcatcga gcgcgtggcg ggcgacatcg tgctcgacgc ctcggtgctg cgcctgccgc
    12661 cgcacgaccc cgacgccttc gatggccgcg gcgtgcgccc ctacaacagc ggtccgtacg
    12721 gcctgttgct gcaatacaac accctgctgc tcggcctgac cccgggcaag gcggccaacg
    12781 cgccggtcgc cctggccgcc gagccgccgc tcgccggcct cgtcatcgac aaccgcgtcc
    12841 tcacctcgga cgccccttgc ggcagctggt accaggacct gcaggccgtg gtcgaacccg
    12901 gtcgccgcct ggtactgagc ggcagcctgc ccgccgcctg cggcccgcgc acctggagcg
    12961 ccgcgccgct gccgccggcc gaattcggca gcgcgctggt cgccgcgttg tggagcgagg
    13021 tcggcggaca cctgggcggg acggtgcgcg aaggccgcct gccgaccggg gcggaagaac
    13081 tgttctccga tgactcggat ccactcgcgg acgtggtgcg cgacatgaac aagtggtcga
    13141 gcaatgtgat cgcgcgccag ctgcttgcca cgctgggccg ggtgagcctg cgcgaggacg
    13201 cgaccgagga catggtgacc gcgggcgcac gcgcggccgc cgcgacgctg gccgccgccg
    13261 gcatcgacac cgacgggctg gtgatcgaga acggctcggg gctgtcgcgg atcgagcgca
    13321 tccgcgccga cagcctggga atgctgctgc tggccgcctg gcagcggccg tggatgccgg
    13381 agttcatcgc cgccctgccg gtggcgggcg tcgacggcac cgcccgccgc cgcctcggcg
    13441 acagccccgc acgcggccag gcccacatca agaccggcac gctcaatggc gtgcgcgcga
    13501 tggccggcta cctgctcgac cgcaatggcc aacgccacgc ggtggtgatg atggtcaacc
    13561 accccgaggc cgccgccagc gccgcggcgc aggacgccct gctggagtgg ctgtggagcg
    13621 gcggggaatg aggcccacgc gcaacggcgg cgccgcattc gcgccttccg gcgctcctgc
    13681 gggtcaggaa gcaccgcggc tcctgtagga gcggcgcaag ccgcgaatgc ggcgctccgg
    13741 caagcgctgc ggcaagcaca ggcgcccgct ggctcgcgcc agccacgact cctccatcgc
    13801 gacgtcacgc catcggcatc gtctgccgct gcatcaggtt ccaccacccg cgcaccacgc
    13861 ggtagaggat ccacagcccg gcgatcaaca gcagcgcgag cgcgaagggc acgccgatga
    13921 tggtcaccac catcagcacc gacaccgcga tccacagcac cgcgaaccag aaggtgcgga
    13981 tctgccagcg gaagtggctc tccagccagg taccccgcac cgcgttctgg ttccagtagt
    14041 tcaggatcac cgcagcgatc gagggcaggc tggccacgaa gctgaagatg atggtggccg
    14101 agcccaccac cgccgagaac accgcgaagg catgcagcgc atacaccagg tgggtgacgg
    14161 tgaccatgtt ctcggacggc tggccggcct cgagcggggc ggggtaggga ctgttcatcg
    14221 actttcctcg aacggaagcc cgcgccgggg acatccgcag cgcatgacga aggcttcgac
    14281 cgcggaccat gccgggtgtt cggctgcagt gcaacaacaa aacgtaagcc tacagcccca
    14341 gctcgcccca catcgcatcc accttcgcct tcacggccgc atccatcacg atcggcgtcc
    14401 cccactcgcg gctggtctcg cccggccact tgttggtcgc gtccagcccc atcttgctgc
    14461 ccagccccgc caccggactg gcgaagtcga gatagtcgat cggcgtgttg tcgaccagcg
    14521 tggtgtcgcg cgtggcgtcc atgcgcgtcg tgagcgccca gatcacctcc ttccagtcgc
    14581 ggatgttcac atcctcgtcc accacgatga tgaacttggt gtacatgaac tggcgcagga
    14641 agctccagat gc
//
LOCUS       DAOQBS010000076        14308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3293_length_14308_cov_12.910756, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000076 DAOQBS010000000
VERSION     DAOQBS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 14308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14308
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3293_length_14308_cov_12.910756"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PKN25_09755"
     CDS             <1..1299
                     /locus_tag="PKN25_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91226.1"
                     /translation="VVASALKRLAQHAGDFVVSVGGYHPSFQVPAHYPRPERVEFNCQ
                     IGQVTISGQCYFALCPAAIMAGGSLSIVYASGGIRAWLVARADFLMQWKPLHYEAAVA
                     VSLGVQLNIKIWFVRIRLSIELGAAIALYGPPLAGSVRISLYVVSFTVGFGPPKSLPP
                     PMVWESDDPERSFAHSFLGNPDVTRISVVDGLLDTPAAPPGSAPRRPVLQAHRLHLRC
                     QSSVPATELRFDGRELHPRGGGTWPQLGVQPMGLGRFHSRIELTLEALHPDGSVRGDA
                     QAELDLAPLTVSVPSALWSPRPPGIDILSGKTLIDGAPVGIELRGRVDPDTRVGPALE
                     LETFAYDRVEYPCSDVGALRPATALPGSAARLGDTLMAGVVVERRRAIVDCLNAGRGV
                     RKLSADAELPILAAAPEHVLDVEPLMARIGQDVPRMFAEI"
     gene            1328..>3359
                     /locus_tag="PKN25_09760"
     CDS             1328..>3359
                     /locus_tag="PKN25_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91227.1"
                     /translation="MEETAAVPSSSVGPGQIRFADACLPALAAGDYRLRMNQEVRAAP
                     LVPLPMHSVPYASELLFSVDGPRFRLEPADIHATYPPPGAAGAFADALPHVVFTRRTL
                     PWERTLDGGPQGDPPRPWLAVLLLGEDELLAGVEQGEGRAVAACVPPRPLPVCGGADG
                     KEDSLLFARDPQGRNPARIRVPALGQEGPDHERLLARHAGERCQALDLPANLFAAIAP
                     RDVDLPWLAHARLIDTGDKEIFRGHDQGWYAVAIGNRMPAAGGAHHAFLVSLEGFADV
                     LADFSRLDGYTSVRLAVLASWRFECVGKGDFKARMRGLDAGAFERARIAGERAGSEGA
                     GTERTAAGVDAWLHLPLPEPALAAESAPARLPGEAPTPAALVHGALARGYAAFDHRLR
                     HGERTVSWYRGPLVPLEYPQPRLVQRLVRCSDELLRYDPECGMFDTAYAGAWQLGRLL
                     ALQNRGFVLALERARLGLRRRLAELRHAARLASLVRCAGVRGEPDDGGRPGRLREALA
                     LACAEAIEAGVGAGRAGLGPGPTPIAPAASAPTAAPVLRATELEAMLAEASAWLGRLV
                     LLYGVPLHNLVPDEDMLPPETLRFFFLDPNWIQALVQGACSVGNADCGDLLVNRVMRF
                     CLSPTVGDPAAETDLPIEVGAAGEAGRAAASVRARLRAECEGRATASAKASEK"
     assembly_gap    3360..3459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3460..4104
                     /locus_tag="PKN25_09765"
     CDS             <3460..4104
                     /locus_tag="PKN25_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91228.1"
                     /translation="LRAECEGRATASAKASEKASTASPAGLAWPLGGFLLRSAVVEGW
                     RGLEVEAWQGGNKLPALRIEQLADDVMLALFNGMPDTVEIRQPREGLHFGLSPTVGGS
                     GFTRTRRDPQTGAALVGRPIDVPMRGVPEPGANVLGAPGVIDIDALAGVLRPVSAPGV
                     APTASASAPAAAGGAVPDGVVAAQPFGPGAFAMQMIEAAGAFRFQIVAGGARVG"
     gene            4094..8173
                     /locus_tag="PKN25_09770"
     CDS             4094..8173
                     /locus_tag="PKN25_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91229.1"
                     /translation="MSADPSRPFLLVPMNVEALAVGAASAAIDLAADFSALAEGGPCL
                     GSLLAREPFQETPQALAPGVYLHWSLPDGLTHAPESGEGEAVGQRMPAIPNRWLVLRF
                     GAADAACRGWVVESDTLVDVRAGRAWPRRVADASAPLGPDELLVHLGRCVPLEAWSEH
                     HPVRQPGITALGYGDPVFAASFPHGRGVLGFHDGEVCALHEGELHYLVLGWYSAAKDE
                     DPLAVCLDKARSARDWDAWKAFFEGCAWVYPGLDELLVKHEERLEAEDRALQASAHAR
                     RVAQRRRGAVPRDAARWLASEYVEARRQAQARKAELDTAWHHLPGQILCHGLLAGLRW
                     RGPAAVYGGPPRDAPFRAALGEDGAEAMAALLGAQPGVPAEHAAYFEALQHDLDPLLE
                     HPGGAERLAHELHVRRFQPLSRASGWEIAEREPAPLRAGRPARKAIPGIVRRALAALE
                     RQQRAADAVARELDSLGQALYLAWYQRGLASFDCDAEAVERWRAEESVLRARIALQRE
                     RLAERVDAQGMPRGRPLERVRALVECLLPGHELRRVDGVRFHRPRDPVLLLAGAAFAD
                     PGRHGTDGRHRADGRLHCRTSTRLAEGGPAAAKDEAVIGAMAGLDLQRRGIPAPAAGL
                     LREAMRIARADETVVPRADATEAGPSNAPPAGGSGTGAPGSADLAPAAIACTRWRGNP
                     WLPLLLQWRVSWSELAGAGEGAEALAGWRLDARGNGFERSVEAVPGREHVFTGTSLMT
                     PGVADALRRRLAKAGMEVAAIDVLGQNLDGLLDGLLARCARPELPPLEADGPAGPLRP
                     APHAAQLEAAEWLSPLRNASLLPLRAGLLRIERLRVIDAFGQFMELRPEGGGALPVLL
                     PQRLRGPGGAALFEPRLVQAARLVVEWPASGCDEGSKRGGSAREQDDAAEEPGEGEPC
                     GWILPDRVGGTLVVLDGAGNLLGALQAMRGKADAHGVGGRARPIDGFRWVTTRGPHAA
                     TAAEAQGEAMLDPADPLAESGADPVVRAVVRELLRWQGASGARLADLLDTLQQAAGEL
                     LPDADDPAVGLLVGRPLALLRARLRLELDGAPAHDPASVQGGGHCGGVGAIEFPLRLG
                     DFDGSAGDGLAGFFLADEPGHFHPAWGLRTGGGRPELRDRAQPVVSIERPLELILLMD
                     PERGFTVTSAVLPRASFRPPGACERVRARSLMFFTGPVLGARDELRMPRPSDLYGQWS
                     WNHHPRVGVREEREIIDTAASGSVAFPSGLALAEGWLRLEAVALAIDGFGVVEAATAA
                     SAGGAAQDAAEGFELPRGVPATLVWTVSGADAIELSAPDGRVLLCTAVAPLPSTWVLA
                     HPQAGRYRLRAWRRLATSDRDSPPACRIAEVALRLRRRA"
     gene            8170..10041
                     /locus_tag="PKN25_09775"
     CDS             8170..10041
                     /locus_tag="PKN25_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91230.1"
                     /translation="MTDDAMGLLARLDSLGDFVVGARSAANRLTLRIDYRGDAALART
                     RWPALVLRGGVGGGEGDLVRSAAQLRRWTCQAPAGWVAQWRLPPQRPERCELWLLRRG
                     EVLLRPGDALEVVLSGAVSDAQPGLAALALGLQASGDAAATAAIALRLAKRASAAGVL
                     DFACVPDQAAVNLPGDVVELRWRTCGLAGLSLLRDGLCIAEGVPDEGGRRVVVGVADA
                     EFVLRGWDGAGERHEARLRVRALRPGWQRWPCVLATREGRRLAMPALADTSAQAVPLR
                     LFGIAGGKLYASVALPGGGGSRVLLCRTHHALGPWEPVGAQGVTEGATAVATEGAGPF
                     VAFPPGCERLPGALFRGRLWLFGGMSGTEGRCGNKVWSLDVGAREPRWRDHGEASWCA
                     RRAHGVAVFEDRLWVFGGIDSAGAPLDDAWAATQKPAEDEKPAENAAGPTLDWKEQPA
                     GGPRERPRGAAQPVVQGGRVWLFGRTGSKCAAEEGCEPGRESDQERSECFDLWSFDGQ
                     RWERSFLLGDLIDGRVLAAALVSDGCSLHVFAWLDRCGEPDGGDAERVEAIALRLVDA
                     PAKRWAVLPCGDLQRLVPAEIEDFQVQALRAGALVVHLHPAENECAADRALRLYFPA"
     gene            10078..11928
                     /locus_tag="PKN25_09780"
     CDS             10078..11928
                     /locus_tag="PKN25_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91231.1"
                     /translation="MKLRSLHICLALLALVPAMLPAAERTVAAAEPGSVAFAGSSAVS
                     TTLLEARDPPPASTAEAATGGEHYWLRPGNGVRLAFDLDSLPVGLSAAQMRRCVLRVV
                     ASEVRYVREHEAVAAVVELAVRRVGETRPLAALRDLRPDRPVSLNPDNGRLCEAVAEA
                     AAAPARRLELELYTATAKASSLLHGRAPAEDTIRHASSAPRLVIQYAPDAVAAAAAKG
                     WHQPQHDAGHSGRGDSNPAHGPSGFELQALPLPAVGEDAGPGVLAERPLLWRDQVIAV
                     LKRGKRNFLVVLDGMGALLWSSEIGGGVVQRAPVIGPQGLLYLVTEDAVRAWDLEARG
                     ALRHRFVLPELAPQMPVRLAPFTKPTVGEDGSLYLVLGDGVDGVLGLDARLRPFLFFG
                     LHEDAGTITPGSPALSIDGRYVAVPNGEGAVLIDLHDPAEAERLAFGDGAAGSGWRHH
                     LPVAGMRAGEWIFSGYDAEDGTGTVWAFAGRAPRWQASGGTSTQPVLAPDGRVCFVQD
                     GTLNVRGFGPGDAGRRFGDGLCASSNPVMDGAGNLFFWCSGTLHGFAPDGRALFAGQR
                     FADGPEENLALALGADGSLWTANANGGELFVFRPRFSPPPAPPAARHQRR"
     gene            complement(12054..13691)
                     /locus_tag="PKN25_09785"
     CDS             complement(12054..13691)
                     /locus_tag="PKN25_09785"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HNV91232.1"
                     /translation="MSIQTVPTRPFPGQRPGTSGLRKKVAVFQQPHYLENFVQAVFDT
                     LPGHEGQTLVLGGDGRFHNRVAVRTILRMAAANGFARVLVGRGGLLSTPAVSAVIRGR
                     GAFGGLILSASHNPGGPEGDFGIKYNAANGGPAPEKLTEAIYARSTEIAEYRIEDAGA
                     DIDFDTLGTRTLGGMTVEVIDPVADYAELMQQLFDFDAMRAWFAAGHRMRFDAMSAVS
                     GPYARAILEGRLGAPAGTVINGEPLEDFGGHHPDPNPAHAAELMAAMSGPGAPDFGAA
                     SDGDADRNMILGRDFVVTPSDSLAVLAEHATRVPGYRAGLAGIARSMPTSRAADKVAA
                     ALGIPCHETPTGWKFFGNLLDAGQATLCGEESYGTGSNHVREKDGLWAVLFWLNLLAT
                     TGRSVEDLVRAHWARFGRHYYSRHDWEGIPSERADALMAELRASLPALAGRDCGDGLR
                     IASADDFAYTDPVDGSVSTRQGVRLLFADGSRIVFRLSGTGTEGATLRVYLERFDADP
                     ARHDQPVQQALGALIRIADEVAGIRRHSGMEGPTVVT"
     gene            complement(13893..14048)
                     /locus_tag="PKN25_09790"
     CDS             complement(13893..14048)
                     /locus_tag="PKN25_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91233.1"
                     /translation="MFNLFVAAVVAPAAALIVIAWSGKLGWPPLLGLIVAVAAGLGII
                     SDRIRKP"
BASE COUNT         1700 a         4981 c         5484 g         2043 t
ORIGIN      
        1 gtcgtcgcca gcgcgctcaa acgactcgcg cagcatgcgg gtgatttcgt cgtctcggtg
       61 ggtggctacc atccatcctt ccaggttccg gcgcattacc cgcgaccgga gcgggtcgag
      121 ttcaactgcc agatcggtca ggtcacgatc agcgggcagt gctacttcgc cctgtgtccc
      181 gcggcgatca tggccggcgg cagcctgtcg atcgtctacg cttcgggcgg catccgcgcc
      241 tggctggtcg cgcgcgcgga tttcctgatg cagtggaagc cgctccacta cgaggccgcg
      301 gttgcggtct cgctgggcgt gcagctgaac atcaagatct ggttcgtgcg catccgcctg
      361 tcgatcgagc tcggcgcggc gatcgcgctc tatggtccgc cgctcgccgg cagcgtgcgt
      421 atctcgctct acgtggtcag cttcacggtc ggcttcgggc cgcccaagag cctgccgccg
      481 ccgatggtct gggagtcgga cgaccccgag cgctcgtttg cgcacagctt cctcggcaac
      541 cccgacgtca cccgcatctc ggtcgtggat ggcctgctcg atacgcccgc cgcgcccccg
      601 ggcagcgcgc cccggcgccc ggtgctgcag gcgcatcgcc tgcacctgcg ctgccagagc
      661 agcgtgccgg cgaccgagct gcgcttcgac ggccgcgagc tgcacccgcg cgggggtggc
      721 acctggccgc agctgggcgt gcaaccgatg ggcctggggc gctttcattc acggatcgag
      781 ctcacgctcg aggcgctcca cccggatggc agcgtccggg gagacgcgca ggccgaactc
      841 gacctcgcgc ccctcaccgt cagtgtcccc agcgcgctgt ggagtccgcg cccgcccggc
      901 atcgacatcc tgtccgggaa aaccctcatc gacggcgcgc cggtgggcat cgaactacgc
      961 ggccgggtcg atccggacac aagggtcggc ccggcgctcg agctggagac cttcgcgtac
     1021 gatcgggtcg agtatccctg cagcgatgtc ggtgcgctcc gcccggcgac ggccctgccc
     1081 gggagcgcgg cgaggctggg cgacaccctc atggcgggcg tcgtggtcga gcgccgcagg
     1141 gcaatcgtgg actgcctcaa cgccggccgc ggcgtgcgca agctgagcgc cgacgccgag
     1201 ctgcccatcc tcgccgcggc gcccgagcac gtcctcgacg tcgagcccct gatggcgcgg
     1261 atcggccagg acgtaccgcg catgttcgcc gagatctgat cgcctcgata tgggagattc
     1321 cgctgtcgtg gaagagaccg ccgccgttcc gtcgtcaagt gtcgggcccg ggcagatccg
     1381 cttcgccgat gcctgcctgc ccgcgctcgc ggcgggcgac taccgcctgc gcatgaatca
     1441 ggaggtacgc gcggcgcccc tcgttccctt gccgatgcac tcggtgccct atgcgtccga
     1501 actgttgttc agcgtcgatg ggccgcgctt ccgcctcgag ccggcggaca tccacgccac
     1561 ctatccaccg ccgggtgcgg ccggcgcctt cgccgacgcg ctgccacacg tggtgttcac
     1621 ccgccgcacg ctgccttggg agcgcacgct cgacgggggg ccgcagggcg atccgccacg
     1681 accctggctc gccgtgctgc tgctcggcga ggacgagttg ctcgccggcg tggaacaggg
     1741 cgagggcagg gcggtggcag cctgtgtccc gcctcgtccg ctgccggtgt gcggcggtgc
     1801 ggacggcaag gaggacagcc tgctcttcgc gcgcgacccg cagggccgga atcctgcccg
     1861 gatccgggtt cccgcactcg gccaggaggg gcccgaccac gagcggctgc tggcccgcca
     1921 cgctggcgag cgctgccagg cgctggacct gccggcgaat ctgttcgccg cgatcgcgcc
     1981 ccgggatgtg gacctgccct ggctggccca tgcccggctg atcgacaccg gcgacaagga
     2041 gatcttccgc gggcacgacc agggctggta tgcggtggcg atcggcaatc gcatgccggc
     2101 cgcggggggc gcgcatcatg ccttcctggt ctcgctggag ggcttcgcgg atgtgctcgc
     2161 cgacttcagc cgcctcgacg gctacaccag cgtgcgcctc gcggtgctcg cgagctggcg
     2221 tttcgagtgc gtcggcaagg gcgacttcaa ggcacggatg cgcgggctcg atgcgggggc
     2281 cttcgagcgc gcacggatcg cgggcgagcg cgccgggagc gagggagcgg ggaccgaaag
     2341 gacggcagcc ggggtcgacg cctggctcca cctgccactg cccgagccgg ccctggcggc
     2401 cgaatcggcg ccggcgcgcc tgcccggaga ggctccgacg ccggccgcgc tggtgcatgg
     2461 cgcgctcgcc cgcggctacg ctgcgttcga tcatcggctc cgccacggtg agcggacggt
     2521 gtcctggtat cgcggtccgc tcgtgcctct ggaatacccg cagccgcgcc tggtgcagcg
     2581 gctggtgcgc tgcagcgacg agctcctgcg ctacgacccc gagtgcggca tgttcgacac
     2641 cgcctacgcc ggtgcctggc agctcggtcg cctgctcgca ctgcagaacc gcggcttcgt
     2701 gctcgcgctc gagcgtgcgc ggctgggcct gcggcggcgg ctcgccgagc tgcgccacgc
     2761 cgcccgcctg gccagcctcg tgcgctgcgc gggcgtgcgc ggcgagcccg acgatggcgg
     2821 gcggcccggg cggctgcgcg aggcgcttgc gctggcttgc gcagaggcga tcgaggctgg
     2881 cgtcggggcc ggacgtgccg gcctcgggcc ggggccgacc cccatcgcgc ccgcggcgtc
     2941 ggcgcccacg gcagcgccgg tgctgcgggc gaccgaactc gaggccatgc tcgccgaggc
     3001 cagcgcctgg ctcggccggt tggtgctgct ttacggcgtg ccgctgcaca acctggtgcc
     3061 cgacgaggac atgctgccgc cggagaccct gcgcttcttc ttcctcgacc cgaactggat
     3121 ccaggcgctg gtgcaggggg catgcagcgt gggcaacgcg gattgcggcg atcttctggt
     3181 caaccgggtg atgcgcttct gcctgtcgcc cacggtgggc gatcccgccg cggagaccga
     3241 cctgcccatc gaggtgggcg ccgcggggga ggccggacgc gccgcggcga gcgtgcgcgc
     3301 gcgcctgcgt gccgaatgcg aggggcgcgc aacggcctcg gcaaaagcct cggaaaaggn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tgcgtgccga atgcgagggg
     3481 cgcgcaacgg cctcggcaaa agcctcggaa aaggcctcga cagcgtcgcc tgccggcctg
     3541 gcctggccgc tcggtggctt cctgctgcgc tcggcggtgg tcgaaggctg gcgcggcctg
     3601 gaggtcgagg cgtggcaggg gggcaacaag ctgcctgcgc tgcgcatcga gcagctggcc
     3661 gacgacgtca tgctcgcgct cttcaacggc atgccggaca cggtcgagat ccgccagccg
     3721 cgcgagggcc tgcacttcgg gctcagcccg acggtcggtg gcagtggctt cacgcgcacg
     3781 cggcgcgacc cgcagaccgg cgcggcgctc gtcgggcgcc cgatcgatgt gccgatgcgc
     3841 ggcgtgccgg agccaggcgc gaatgtcctc ggggccccgg gggtgatcga catcgatgcg
     3901 ctcgccggtg tgctccgtcc ggtgtctgca cctggcgtcg cgccgaccgc gtccgcctcc
     3961 gcacctgccg ccgctggcgg cgccgtgccc gacggagtcg tcgccgcgca gcccttcggc
     4021 cccggcgcct tcgcgatgca gatgatcgag gcggcggggg ccttccgttt ccagatcgtt
     4081 gccggaggtg cgcgtgtcgg ctgacccctc ccgtcccttc ctgctcgtgc cgatgaacgt
     4141 cgaggcgctc gcggtgggtg cggcgagtgc ggcgatcgat ctcgcggccg atttttccgc
     4201 gctcgccgag ggggggccct gcctgggcag cctgctcgcc cgcgagcctt tccaggagac
     4261 gccgcaagcg ctcgcacccg gggtctatct gcactggtcg ctgcccgacg gcctcaccca
     4321 cgccccggag tcgggcgagg gcgaggccgt cgggcagcgc atgccggcga ttcccaaccg
     4381 ctggctcgtg ctgcgtttcg gggctgccga tgccgcgtgc cggggctggg tggtcgagag
     4441 cgacacgctg gtcgatgtgc gcgcggggcg tgcgtggccg cgcagggtcg ccgacgcgtc
     4501 ggcgccactc gggccggacg agttgctcgt gcacctgggg cgctgcgtgc cgctcgaggc
     4561 ctggtccgaa caccaccccg tgcgccagcc cggcatcacc gcgctcggct atggcgatcc
     4621 ggtgttcgcg gcgagcttcc cgcacgggcg cggggtgctc ggcttccacg acggggaggt
     4681 gtgcgccctg cacgagggcg aactccacta cctcgtgctc ggctggtact ccgccgccaa
     4741 ggacgaggat ccgctcgccg tctgcctgga caaggcgcgc tcggcgcgcg actgggatgc
     4801 atggaaggcc ttcttcgagg gctgcgcatg ggtgtatccg gggctcgatg aactcctggt
     4861 caagcacgag gagcggctcg aggcagagga tcgggcgctg caggccagcg cgcacgcccg
     4921 ccgcgtcgca cagcgtcgcc gcggtgccgt gccgcgcgac gcggcgcgct ggctcgcgag
     4981 cgagtatgtc gaggcgcggc gccaggcgca ggcgcgcaag gccgagctcg acacggcctg
     5041 gcaccacctg ccggggcaga tcctgtgcca tggcctcctc gccgggctgc gctggcgcgg
     5101 cccggcagcg gtgtacggcg gcccaccgcg cgatgccccc ttccgcgcgg ccctgggcga
     5161 ggacggcgcc gaggccatgg cggcgctgct cggggcccag cccggcgtgc cggccgagca
     5221 cgccgcctac ttcgaggcat tgcagcacga cctggatccg ctgctcgagc atccgggcgg
     5281 ggccgagcgc ctcgcccacg aactgcatgt gcgccgcttt cagccgctgt cgcgcgcctc
     5341 cggctgggag atcgccgagc gcgagccggc cccgctgcgt gccgggcgcc ctgcacgcaa
     5401 ggccatcccc ggcatcgtgc ggcgtgcgct cgccgccctg gagcgacagc agcgcgcggc
     5461 cgacgccgtc gcgcgcgagc tggacagcct cgggcaggcg ctctacctcg cctggtacca
     5521 gcgcggcctg gccagcttcg actgcgacgc cgaggcggtc gagcgctggc gcgccgagga
     5581 gtccgtgctg cgcgcgagga tcgccttgca acgcgagcgc ctcgccgagc gggtcgatgc
     5641 gcagggcatg ccgcgcgggc gtccgctcga gcgcgtgcgc gcgttggtcg agtgcctgct
     5701 gcccggccac gagttgcgac gggtcgacgg tgtgcgcttc catcgcccgc gcgacccggt
     5761 cctgctgctc gccggagcgg cctttgccga ccccgggcga catggcaccg acggtcgcca
     5821 tcgcgccgat ggtcggctgc actgccggac cagtacccgc ctcgccgagg gcggccctgc
     5881 cgccgccaag gatgaggccg tgatcggcgc gatggcgggt ctcgatctgc agcgccgtgg
     5941 cattccggca cccgcggcgg gcctgctgcg cgaggcgatg cgcatcgcgc gtgcagacga
     6001 gacagtcgtt ccccgtgcag acgcaaccga agccggcccc tcgaacgcgc cacccgccgg
     6061 cgggtccgga accggtgctc ccggcagcgc ggacctggcc cccgcggcga tcgcctgcac
     6121 gcgctggcgt ggcaacccct ggctgccgct gctgctgcag tggcgggtgt cgtggagcga
     6181 gctcgccggg gccggggagg gggccgaggc gctcgcgggg tggcggctcg atgcgcgcgg
     6241 caacgggttc gagcgcagcg tcgaggcggt ccccgggcgc gagcacgtct tcaccggcac
     6301 ctcgctgatg acccccggcg tggccgacgc gctgcggcgc cgcctggcca aggccggaat
     6361 ggaggtggcc gcgatcgacg tcctcgggca gaacctcgac ggcctgctgg acggcctgct
     6421 ggcccgctgc gcacgacctg aacttccccc gctcgaggcc gacggcccgg cggggccgct
     6481 gcgtcccgcg ccgcatgccg cacagctcga ggctgcagaa tggctgtcgc cgctgcgcaa
     6541 cgcgagcctg ctgccgctgc gcgccggcct gctgcgcatc gagcgcctgc gggtgatcga
     6601 cgccttcggc cagttcatgg agctgcggcc cgagggcggc ggtgcccttc cggtgctgct
     6661 gccgcaacgc ctgcgcgggc ccggcggggc tgcgctgttc gagccgcgtc tggtgcaggc
     6721 cgcccgcctg gtggtggaat ggcctgcaag tgggtgcgac gagggcagca agcgcggcgg
     6781 aagcgcgcgg gagcaggacg acgccgcaga ggagcccggc gagggcgagc cgtgcggctg
     6841 gatccttccc gatcgggtcg gtggcacgct cgtggtgctc gacggcgcgg gcaatctgct
     6901 cggcgccctg caggcgatgc gcggcaaggc ggacgcgcac ggcgtcggtg gacgcgcccg
     6961 cccgatcgac ggcttccgct gggtcacgac gcgcggtccg cacgccgcca cggcagcgga
     7021 ggcgcagggc gaggccatgc tcgacccggc cgatcctctg gccgaaagcg gcgccgatcc
     7081 ggtcgtccgt gcagtcgtgc gcgagctgct gcgctggcag ggggcgagcg gggcgcgtct
     7141 cgccgacctg ctcgacacct tgcagcaggc ggcgggcgaa ctcctgcccg acgccgacga
     7201 ccccgctgtc gggcttctcg tcggacggcc gctggcgctg ctgcgcgcgc gcctgcggct
     7261 cgagctcgat ggcgcacctg cccacgaccc ggcgagtgtt cagggtggag gccactgtgg
     7321 cggagtcggg gcgatcgagt ttccgctgcg cctgggtgac ttcgacggca gcgcggggga
     7381 cgggctcgcg ggcttcttcc tggccgacga gccggggcat ttccacccgg cctggggcct
     7441 gcgcaccggc gggggaaggc ccgagctgcg cgaccgggcc cagcccgtgg tcagcatcga
     7501 gcgcccgctc gagctcatcc tcctgatgga cccggagcgc ggtttcacgg tgaccagcgc
     7561 ggtcctcccg cgcgcgagct tccggccgcc cggcgcgtgc gagcgcgtgc gtgcgcgttc
     7621 gctgatgttc ttcaccggcc cggtgctggg tgcgcgcgac gagctgcgca tgccgcggcc
     7681 gtcggacctc tatgggcagt ggtcgtggaa ccatcacccg cgcgtgggcg tgcgcgagga
     7741 gcgcgagatc atcgacaccg cggcgagcgg cagcgtcgcc ttcccgtccg ggctcgcgct
     7801 ggccgagggc tggctgcggc tcgaagcggt cgcgctggcg atcgacggct tcggggtggt
     7861 cgaagctgcg accgccgcgt cggcgggcgg ggccgcgcag gacgcggcgg agggcttcga
     7921 gcttcccagg ggcgtgcccg ccacgctagt ttggacggtg agcggcgccg acgccatcga
     7981 gctgagcgca ccggacgggc gcgtcctgct gtgcaccgcg gtggcgcccc tgccctcgac
     8041 ctgggtgctg gcgcatccgc aggccggccg ctaccgcctg cgcgcatggc gtcggctcgc
     8101 cacctcggac cgcgactcgc caccggcgtg ccgcatcgcc gaggtggcgc tgcgactgcg
     8161 gaggcgggca tgacggacga cgccatgggg ctgctggccc gcctcgacag cctgggcgac
     8221 ttcgtcgtcg gcgcgcgttc ggcagccaat cggctgacgc tgcggatcga ctaccgcggc
     8281 gacgcggcgc ttgcgcgcac gcgctggccg gcgctcgtcc tgcgtggcgg ggtcggcggc
     8341 ggggagggcg acctggtgcg gagcgcggcg caactgcggc gctggacctg ccaggcgccg
     8401 gcgggctggg tggcgcagtg gcggctgccg ccgcagcggc cggagcgctg cgagctatgg
     8461 ttgctgcgcc gcggcgaagt cctgctgcgg cccggcgacg ccctcgaggt ggtcctgtcg
     8521 ggcgcggtct cggacgcgca gcccggcctt gcggcgctcg cgctcgggct gcaggcgagc
     8581 ggcgatgcgg ccgccacggc ggcgattgcg ctgcggctcg ccaagcgtgc atcggccgcg
     8641 ggcgtgctcg actttgcctg cgtgccggac caggccgcgg tgaacctgcc cggcgacgtc
     8701 gtcgagctgc gctggcgcac ctgcgggctg gcgggcctga gcttgctgcg tgacggcctg
     8761 tgcatcgccg aaggtgtccc ggacgaaggg gggcggcgcg tcgtggtggg ggttgcggat
     8821 gccgagttcg tgctgcgggg ctgggacgga gcgggcgagc gtcacgaggc gagattgcgc
     8881 gtgcgcgcct tgcggccggg ctggcagcgc tggccctgcg tgctggcgac gcgggagggc
     8941 cgcaggctgg ccatgcccgc cctcgccgat acgagcgcac aagcggtgcc gctgcgcctg
     9001 ttcggaattg ccggcggcaa gttgtacgcg agcgtcgcgt tgcccggggg gggcggctcg
     9061 cgcgtgctgc tgtgtcgcac gcaccatgcg ttggggccct gggagccggt gggtgcgcag
     9121 ggggtgaccg agggggcaac tgcggtggca actgaggggg caggcccgtt cgtggccttc
     9181 ccgcctggct gcgagcgctt gcccggggcc ttgttccgcg gtcgcctgtg gctctttggc
     9241 ggcatgtccg gcacggaggg gcgctgcgga aacaaggtct ggagcctcga cgtcggtgcc
     9301 cgcgaaccgc gctggcggga tcacggcgag gcctcctggt gcgcccggcg ggcacacggc
     9361 gtggcggtgt tcgaggatcg cctgtgggtg ttcggcggca tcgactccgc cggcgcgccg
     9421 ctcgacgacg cctgggcggc gacgcagaag cctgccgagg acgagaagcc tgccgagaac
     9481 gccgcggggc cgaccctgga ctggaaggag cagcccgcgg gggggccgcg cgagcgtccg
     9541 cgcggcgccg cccagccggt ggttcaaggg gggcgggtgt ggctgttcgg caggacagga
     9601 tcgaagtgtg ccgcggagga gggctgcgaa ccggggcggg agtccgacca ggaacgcagc
     9661 gagtgtttcg acctgtggtc tttcgacggc cagcgctggg agcgcagctt cctgctcggt
     9721 gacctgatcg acgggcgggt gcttgccgcc gcgctggtga gcgacgggtg cagtctgcac
     9781 gtcttcgcat ggctggaccg ttgcggcgaa ccggatggcg gcgacgccga gcgggtcgag
     9841 gccatcgcgc tgcgcctggt ggatgcgccc gcgaagcgct gggcagtgct gccctgcggg
     9901 gatctgcagc gcctggttcc ggcggagatc gaggatttcc aggtgcaggc gctacgcgcc
     9961 ggcgcattgg tggtccacct gcaccccgcg gagaacgaat gcgcggcgga ccgtgcgctg
    10021 cgactgtact tcccggccta ggcgcgacgc cgccgggatg cgcaagggga ggacgggatg
    10081 aagctgcgat cactgcacat ctgcctggcg ctgctcgcgc tggtgcccgc catgctgccg
    10141 gcggccgagc gcacggtggc tgcggcggaa ccggggagcg tggcgtttgc gggcagctcg
    10201 gcggtttcca cgaccctcct cgaggcgcgc gatcccccgc ctgcatcgac ggccgaggcg
    10261 gcgacagggg gtgagcacta ctggcttcgg cccggcaacg gcgtgagact ggcgttcgac
    10321 ctcgacagcc tgcccgtcgg cttgtcggcg gcgcagatgc ggcgctgcgt gctgcgcgtg
    10381 gtggcgagcg aggtgcgata tgtgcgcgag cacgaggcgg tcgccgccgt ggtcgagctg
    10441 gcggtgcggc gcgtgggcga gacgcgtccg ctcgccgcgc tgcgcgacct gagacccgac
    10501 cgtccggtgt ccctgaaccc cgacaacggc cgcctgtgcg aggccgtcgc cgaggcggcc
    10561 gcggcgccgg cccgtcgcct cgagctcgag ctctacactg ccaccgccaa ggcgagcagc
    10621 ctgctgcacg gccgggcgcc ggcggaggac acgattcgcc acgcctcgag tgcgccgcgc
    10681 ctggtcatcc agtacgcgcc cgacgcggtg gcggccgcgg ctgcgaaggg ctggcaccag
    10741 ccgcagcacg acgccggcca cagcggccgt ggcgattcca acccggcgca cggcccgagc
    10801 ggtttcgaac tgcaagcgct gcccttgccc gccgtcggcg aggacgccgg ccccggcgtg
    10861 ctcgcagagc gtccgctgct gtggcgcgat caggtgatcg cggtgctaaa gcggggcaag
    10921 cgcaattttc tggtcgtgct cgatggcatg ggggccttgc tgtggtcgag cgagatcggc
    10981 ggcggggtcg tgcagcgcgc gccggtcatc ggcccgcagg gcttgctcta tctggtcacc
    11041 gaggatgccg tgcgcgcctg ggacctcgag gcccgcggcg ccttgcgcca ccgctttgtg
    11101 ctaccggagc tcgccccgca gatgccggtg cgcctggcgc ccttcaccaa accgaccgtg
    11161 ggcgaggacg gcagcctcta cctcgtgctc ggggacggcg tggatggcgt gctcggcctc
    11221 gacgcgcgat tgcgcccctt cctgttcttc ggactgcacg aggatgcggg gacgatcacg
    11281 ccgggcagcc cggcgctgag catcgatggc cgatacgtgg cggtcccgaa cggcgagggc
    11341 gcggtactca tcgatctgca cgaccccgcc gaggccgagc gcctcgcctt cggcgacggc
    11401 gcagccgggt ccggctggag gcatcacctg ccggtggccg gcatgcgcgc gggcgagtgg
    11461 atcttctccg gctacgacgc ggaggacggc acgggaaccg tgtgggcctt cgccgggcgc
    11521 gcgccgcgct ggcaggcgag cggcgggacg agcacgcagc cggtgctggc gcccgacggc
    11581 cgcgtgtgct tcgtccagga tggcacgctg aacgtgcgtg gattcgggcc gggcgatgcc
    11641 ggccggcgct tcggtgacgg gctgtgcgcg agctcgaatc cggtcatgga tggcgcaggc
    11701 aacctcttct tctggtgcag cggcacgctc cacggcttcg cgcccgacgg gcgggccttg
    11761 ttcgccgggc agcgcttcgc agacgggccg gaagagaacc tcgcgctcgc gctcggtgcg
    11821 gacggcagcc tgtggaccgc caacgcgaac ggaggcgaac tgttcgtctt ccggccgcgc
    11881 ttctcgccac cgccggcgcc gcctgcggcc cggcaccagc ggcgttgatg cggcgcaggc
    11941 gtcgttgaga cggtgcggcg cgctgaagcg ccgcgcgtcg taccgtccag acagtgccgg
    12001 aagttgcgca gcgtcttgtg aacggactgg tgcggggcag cgccggctgg ccgtcaggtc
    12061 accaccgtcg gcccttccat gccgctgtgc ctgcggatgc cggcgacctc gtcggcgatg
    12121 cggatgagcg cgccgagcgc ctgctgcacc ggctggtcgt ggcgggccgg gtcggcatcg
    12181 aaccgctcca ggtacacgcg cagcgtggcg ccctcggtgc cggtgcccga caggcggaac
    12241 acgatgcggc tgccgtcggc gaacagcagg cgcacgccct ggcgggtgct gaccgagccg
    12301 tcgaccgggt cggtgtaggc gaagtcgtcg gcgctggcga tgcgcaggcc gtcgccgcag
    12361 tcgcgtccgg cgagcgcggg caggctcgcg cgcagctcgg ccatcagcgc gtcggcgcgc
    12421 tcgctgggga tgccctccca gtcgtggcgc gagtagtagt ggcggccgaa gcgcgcccag
    12481 tgcgcgcgca ccagatcttc caccgagcgc ccggtggtgg cgagcaggtt gagccagaac
    12541 agcaccgccc acaggccgtc cttttcgcgc acgtggttcg agccggtgcc gtagctctcc
    12601 tcgccgcaca gcgtggcctg gccggcgtcg agcaggttgc cgaagaactt ccagccggtg
    12661 ggcgtctcgt ggcaggggat gccgagcgcc gcggcgacct tgtcggcggc gcgcgaggtc
    12721 ggcatcgagc gcgcgatgcc ggccagcccg gcgcgatagc cgggcacccg ggtggcgtgc
    12781 tcggcgagca ccgccaggct gtcggacggg gtgaccacga agtcgcggcc gaggatcatg
    12841 ttgcggtcgg cgtcgccgtc cgaggccgcg ccgaagtcgg gcgcgccggg gccggacatc
    12901 gccgccatca gctcggcggc gtgcgccgga ttggggtcgg gatggtggcc gccgaaatcc
    12961 tccagcggct cgccgttgat cacggtgccg gcgggggcgc cgagccggcc ttcgaggatc
    13021 gcgcgcgcgt acggcccgct caccgcgctc atggcgtcga agcgcatgcg gtggccggcg
    13081 gcgaaccagg cgcgcatggc gtcgaagtcg aagagctgct gcatcagctc ggcgtagtcg
    13141 gcgaccgggt cgatcacctc cacggtcatg ccgcccaggg tgcgcgtgcc gagggtgtcg
    13201 aagtcgatgt ccgcgcccgc gtcctcgatg cggtactcgg caatctccgt gctgcgcgcg
    13261 tagatcgcct cggtgagctt ctccggcgcg gggccgccgt tggcggcgtt gtacttgatg
    13321 ccgaagtcgc cctccggccc gcccgggttg tggctggccg acaggatcag cccgccgaag
    13381 gcgccacgac cgcggatcac cgcgctcacc gccggcgtcg acagcagccc accccggccc
    13441 accagcacgc gggcgaagcc gttggccgcc gccatgcgca ggatggtgcg gaccgcgacg
    13501 cggttgtgga agcggccgtc accccccagc accagggtct ggccttcgtg gccgggcagg
    13561 gtgtcgaaca ccgcctgcac gaagttctcg aggtagtgcg gctgctggaa gacggcgacc
    13621 ttcttgcgca ggccggaggt gccggggcgc tggccgggga aggggcgggt ggggacggtc
    13681 tggatgctca tggatgctcg cggcggtttg tgggcgcgga aggcctggcg gtggccgacg
    13741 cgggtcgcgt cggcgcgtcg tggaggggcg gcctcggaag gccggtattg aacgtgtttg
    13801 cggcgaagtt ctcaagtgct gcgcgggctc ggcgcgtgcg tgcagccacg cgggacgatg
    13861 ggacgccgcc gcggtgcatc gcggtcagcg gctcatggct tgcggattcg atcgctgatg
    13921 atgcccaagc ctgccgccac ggcgacgatc aaacccagca gcggcggcca gccgagcttt
    13981 ccgctccagg cgatcacgat cagcgctgcc gcgggcgcga cgaccgccgc gacgaacagg
    14041 ttgaacaagg cattccacat ttcggcaccc catcggcggt ggtccggacg agcgcagggg
    14101 cgggaaggct tgatcgggat gggttaatcg tggtctgtcc ccgattttcc cacatgtaga
    14161 cgagagcgga cgcaccgacc ggctcttcga ggcgctggcc tcgtatctgg aacgacggga
    14221 cgacagcgcg gtcaaggcgt gacgcgtcgt cgttcggcgg ggggcgtccc cgatgctcgc
    14281 cttgatctga gccatagcga gcaaggct
//
LOCUS       DAOQBS010000077        14186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3340_length_14186_cov_16.994976, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000077 DAOQBS010000000
VERSION     DAOQBS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 14186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14186
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3340_length_14186_cov_16.994976"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PKN25_09795"
     CDS             complement(<1..1101)
                     /locus_tag="PKN25_09795"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNV91234.1"
                     /translation="MSVQRVLTGITTSGTPHLGNYAGAIRPAVAASRQPGVESFYFLA
                     DYHALIKTTDPLRVQRSTLEIAATWIAAGLDTDRVWFYRQSDIPEIPELTWLLTCVCG
                     KGLLNRAHAYKAAVDKNTAEGVDPDAGVNMGLYMYPVLMAADILMFKAHSVPVGRDQI
                     QHIEMARDMAGSFNHLYGEHFVLPEAAIDASVATLPGLDGRKMSKSYDNTIPLFAPTA
                     ELKKLIFSIVTDSKAPGEPKDADGSALFQLYQAFSTAEETRAMRTAFDEGIAWGEAKQ
                     ALFEKIEAAVAPMREHYLALIAEPARIEKHLHEGAAKARAIATPFLGELRRAVGLRNL
                     ASVAGEAKAEKAKTAQPQFKQYREADGRFHFKL"
     gene            complement(1183..1584)
                     /locus_tag="PKN25_09800"
     CDS             complement(1183..1584)
                     /locus_tag="PKN25_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91235.1"
                     /translation="MKPCKRVFAALALLALAAPGWTQPVVDDAPVLRLECGGIGLEES
                     ERMRAEIGMHALTVFFSTTAGGWVTDVETRVDEPLSGQRVEASCGPIGQVDVPAPGRY
                     RISASYAGARQEHWVDLVPQGGARLSLRWQE"
     gene            1868..2734
                     /locus_tag="PKN25_09805"
     CDS             1868..2734
                     /locus_tag="PKN25_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV91236.1"
                     /translation="MKTLLLALALSAVGTAQAAVCQLSDVKVTGVSIYSGTTRDAALA
                     PIPIDTLPVSAAACAGAFSGNDGHYPQTNLGYAGDGLLNGGAQMGSKSVLFPGGAFIS
                     EHYPLQDLRGDGTTDDPGWIMLGKSETQGGRWQFSPSAIGGDRSVVLDSFFSVTETSA
                     GKGTWSFTPDAEAARRAASVLGGNWIDQFAISFKAGNGFALYSFTSALFDAATPKASD
                     PLLNWFGSYDLTETLINRGGNPAGLSHVTLWVRDPATSPDPVPATNAVPEPGSLGLLA
                     LALVGLGLRRRG"
     gene            2993..3613
                     /locus_tag="PKN25_09810"
     CDS             2993..3613
                     /locus_tag="PKN25_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HNV91237.1"
                     /translation="MTQPLTERPRVPIITVGLRPHPTSPDAEAPWQGPVGRLLATMFA
                     LPEGGAVFEFRLEESRPAQLAIPAPATPGPADELWKHTCFEVFLGVPGEAGYREYNFS
                     PSGQWAIYGFRAWRERIEDYIPAAKPEVLFDQTDDGLVLEARVPAELLPAVPAGSDLQ
                     VSLAAVVERKDGMLEYWALRHAAAQPDFHARDTFVLTLATTTQKAA"
     gene            3618..4841
                     /locus_tag="PKN25_09815"
     CDS             3618..4841
                     /locus_tag="PKN25_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNV91238.1"
                     /translation="MAQPVRFGLDRLLEDSALRRPLAGRRVALLAHPASVTRALVHSL
                     DALAALPGLQLTAAFGPQHGLRGDKQDNMVESPDYTDPLLGIPVFSLYGEVRRPTAAM
                     MDSFDVLLVDLQDLGCRIYTFITTLRYVLEEAARLGKSVWVLDRPNPAGRPVEGTLLR
                     DGWESFVGAGPLPMRHGLTMGELAHWFVATLKLDVELRVIEMQGWDPDAAPGFGWPLG
                     ERSWVNPSPNAPNLWMARAYAGTVMLEGTTLSEGRGTTRPLELFGAPDVDARALVTEM
                     RALAPEWLAGCVLRECWFEPTFHKHAAKLCNGVHIHVEDPAHYDHAAFRPWRVQALAF
                     KAIRRLWPDYPLWRDFPYEYEHGRLAIDLINGSPLLREWVDDPAATPAMLDALACPDE
                     AAWVETRRPFLLYPG"
     gene            complement(4829..6451)
                     /locus_tag="PKN25_09820"
     CDS             complement(4829..6451)
                     /locus_tag="PKN25_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNV91239.1"
                     /translation="MNAQASALARLRGDFFGGLTAGIVALPLALAFGVASGAGAAAGL
                     YGAIVLGFVAALLGGTRMQISGPTGPMTVVFASALAVVGGDAALAMAAVLVGGLVQIG
                     LGVLRTGGLVRFIPYPVVSGFMSGIGVIIVLLQSAPLLGVAPASSPLAAVLALPGVLA
                     KVNAEALLLGGLTLLIVFRTPMTVSRIVPAPLVALVAMSALSAAADLQVPVIGDIPAG
                     LPVPALPSFTLETWTTVLLLGITLGVLGSIDSLLTSLVADSITRTRHHSNRELVGQGI
                     GNLLCAFVGGLPGAGATMRTVINIKSGGRGRASGVVHSLFLLALLLGLGPLAERIPLA
                     VLAGILIKVGVDILDYRMLRLVRTAPRADVAVMAAVFALTVLVDLIVAVGAGVILSMA
                     LIIHRLARQSSLRVRPLPHAEEGGQTPAGDELPGLDSGVRVIEIEGAFFFGSASQLLD
                     RVDQVIGTRVVVFDCSRVPFMDLSAQFALEEMIEGLQAQSIAARVVVPPEIHAQLLRL
                     GAPQLPAAILHDELDAALAEARRLAAARPQPG"
     gene            6730..7674
                     /locus_tag="PKN25_09825"
     CDS             6730..7674
                     /locus_tag="PKN25_09825"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNV91240.1"
                     /translation="MIKHGLQIFACNASRELACDISNRLGLRPGRIDISRFSNDNLRV
                     QIQENVRERDVFVVQSFTEPVSEHIMELMITLDALRSASAQRITAVIPYYSYARSDKK
                     DAPRISITGRLIADLLQTAGANRVLTMDLHADQVHGFFSVPVDHLTAVSLIAEHFRNR
                     HDLSNMVAVATDAGGAKRTGRFSEKLGIPMAIIDKRRVSDTAVKQGAVVGDVAGRDVV
                     IFEDEIATAGTLVSSVDTLKAAGARSIHAAATHGVLCGPAIERLRAAAIDSVVVTNTV
                     ALPEDKRLDKLAVLNVAPLFAAAIERIHSGQSVGALFE"
     gene            complement(7687..8598)
                     /locus_tag="PKN25_09830"
     CDS             complement(7687..8598)
                     /locus_tag="PKN25_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNV91241.1"
                     /translation="MIVRPRPHWIHLLFVRRGSLLPRIFSQQLFVLLLSCAVVLAHGQ
                     LFHVKVTLTSAPFSLMGVALAIFLGFRINASYDRYWEARKFWGVVLVEARNLSRHALT
                     LTRADVDARPFVLGLIAFASTMRNQLRGRPRDEGLEGLLPDAVLGRVRSARFAPALVL
                     LWLGQWVRDRREAGCLPPMLAQHMEGSLDALSAALGGCERIANTPLPFTYTVILHRSS
                     YTYCMLLPFGLVDTTGWMTPLVVGFVSYTFFALEALSDEIEEPFGTMTNDLALDAIVA
                     SIDASLRELLGDTPPPAPQPDGDFVLN"
     gene            complement(8738..9211)
                     /locus_tag="PKN25_09835"
     CDS             complement(8738..9211)
                     /locus_tag="PKN25_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNV91242.1"
                     /translation="MEEMRHGPGKAHDNHLGDRVDDPLIDRLHWIIRQAIRVLAVLMV
                     AVILWTVADVVLVLYEKLSDPPFLLLDLKDIFVVFAAFLAVLIAIEIFVNITLYLRDD
                     VIHVKLVIATALMAIARKVIVLDLSTLEPMYLFAIGVIVLALGVTYWLVSARPGE"
     gene            complement(9292..9684)
                     /locus_tag="PKN25_09840"
     CDS             complement(9292..9684)
                     /locus_tag="PKN25_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNV91243.1"
                     /translation="MRHYETNSTPAAARLLALTVLADGGLDRNEVDTMMHADLARRLG
                     IGSVEFERILREYCDDLLLSAHYLDGVRLKVADEVLDLLLEDIADPRLQQALLRTMQD
                     IVSADGVETSAEVEVLARALEKWGSRPH"
     gene            9839..10756
                     /locus_tag="PKN25_09845"
     CDS             9839..10756
                     /locus_tag="PKN25_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV91244.1"
                     /translation="MGSSELNYKHLRYFWTVARVGTIAEAGRVLGLAPHSISAQLATF
                     EGTLGVTLFRRSGRRLALTEAGERILGQAEEIFALGDQIVETLRDDSLRRSLPFRIGI
                     PDSMPKSMVHRLIEPALRLERPGRLVCRESPLSELLAELAVHRLDLVIADRPIPPEVS
                     VRGHEHLLGESTLSVFAAPALAGRLQGDFPRLLDGAPFLLPGEDVAHRGALLQWFERM
                     RIRPAIVAEFDDSALMKAFGQAGDGVFAAPTTIADYVCRQYGVRLLGEVPDARARIYA
                     ITTERRLSHPAMQAIRNAASRALTPAPRA"
     gene            complement(10787..11164)
                     /gene="trxA"
                     /locus_tag="PKN25_09850"
     CDS             complement(10787..11164)
                     /gene="trxA"
                     /locus_tag="PKN25_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNV91245.1"
                     /translation="MATLELTADNFNDTVAHHPIVFLDFWAAWCGPCRNFAPTYEAAA
                     EANPDIVFGKVDTEAQQDLAAAFQIRSIPTIAVIREGVMVFRESGALPANALNQVIEG
                     VRGLDMDQVRAEIAQQQADAQQG"
     gene            11287..12396
                     /locus_tag="PKN25_09855"
     CDS             11287..12396
                     /locus_tag="PKN25_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HNV91246.1"
                     /translation="MRSRAAVAWAAKQPLEVTEVDVAPPKAGEVLVRIVATGVCHTDA
                     YTLSGADPEGIFPAILGHEGGGIIEAVGEGVSSVAVGDHVIPLYTPECGKCKFCLSGK
                     TNLCQAIRATQGKGLMPDGSSRFSKDGKPIFHYMGTSTFSEYTVLPEISVAKIRKDAP
                     LDKVCLLGCGVTTGIGAVRNTAKVEAGASVAVFGLGGIGLSAIIGAVMNKAGRIVAVD
                     INPEKFEIARQLGATDCINPLDYDRPIQEVIVDLTDGGVDYSFECIGNVKVMRSALEC
                     CHKGWGESVIIGVAGAGEEISTRPFQLVTGRVWRGSAFGGVRGRSELPGYVDMAQRGE
                     IPLDTFITHRMGLEDINKAFDLMHEGKSIRSVILY"
     gene            12411..13259
                     /gene="fghA"
                     /locus_tag="PKN25_09860"
     CDS             12411..13259
                     /gene="fghA"
                     /locus_tag="PKN25_09860"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HNV91247.1"
                     /translation="MSTAPEQIAANRSFGGWHRRFRHRSAALGCDMVFAIYLPPQAAA
                     GPVPVLYWLSGLTCTDENFMQKAGAQRLAAELGIAIVAPDTSPRGTGVPGDPDGAWDF
                     GLGAGFYVNATEEPWSRHYRMYDYVVTELPALVEANFPVSDARAISGHSMGGHGALVC
                     ALKNPGRYRSVSAFAPIANPSACPWGEKAFSRYLGAERARWLEWDACALIAGASERLP
                     LLVDQGEADGFLAEQLRPEALRTACAAAGHPLTLRLQAGYDHSYYFIASFIDEHLRHH
                     AAALRG"
     gene            complement(13362..>14186)
                     /locus_tag="PKN25_09865"
     CDS             complement(13362..>14186)
                     /locus_tag="PKN25_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNV91248.1"
                     /translation="FFQTWDLAGRFPAILDDEVVGETARNVFADGKAMLERIVAEEWL
                     QAKAVFGLFPANAAGDDIEIYTGEDRKHLAMVWHGLRQQHERPAGKPHWCLADFVAPK
                     ESGVPDWIGAFAVTAGLGIEARVAEFEAAHDDYHAIMLKSLADRLAEACAEWLHERVR
                     RECWGYAADETLGNEALIREEYRGIRPAPGYPACPDHTVKGPLFELLGARARIGMDLT
                     ESYAMTPAAAVSGFYFAHPESHYFAVSKIGRDQLEDWARRTGMEVDEAARWLAPLL"
BASE COUNT         2207 a         5055 c         4898 g         2026 t
ORIGIN      
        1 cagcttgaag tggaagcggc cgtcggcctc gcggtactgc ttgaactggg gctgtgcggt
       61 cttcgccttc tcggccttgg cctcgcccgc cacgctggca agattgcgca ggcccaccgc
      121 gcggcgcagc tcgccgagga agggggtggc gatcgcgcgc gccttggcgg cgccttcgtg
      181 cagatgcttc tcgatgcgcg ccggctcggc gatcagcgcc aggtagtgct cgcgcatcgg
      241 cgccaccgcg gcctcgatct tctcgaacag cgcctgcttg gcctcacccc aggcgatacc
      301 ctcgtcgaag gcggtacgca tggcgcgcgt ctcctcggcg gtcgagaagg cctggtagag
      361 ctggaacagc gccgagccgt cggcgtcctt gggctcgccc ggcgccttgg agtcggtgac
      421 gatggagaag atcagcttct tcagctcggc agtgggcgcg aacagcggga tggtgttgtc
      481 gtaactcttg ctcatcttgc ggccgtccag cccgggcagg gtggcgaccg aggcgtcgat
      541 ggcggcctcg ggcagcacga agtgctcgcc gtagaggtga ttgaagctgc ccgccatgtc
      601 gcgcgccatc tcgatgtgct ggatctggtc gcgcccgacc ggcaccgagt gcgccttgaa
      661 catcaggatg tcggcggcca tcagcaccgg gtacatgtag aggcccatgt tgaccccggc
      721 gtccggatcg acgccctcgg cggtgttctt gtccaccgcc gccttgtagg cgtgggcgcg
      781 gttgagcagg cccttgccgc acacgcaggt gagcagccag gtgagctcgg ggatctcggg
      841 gatgtcggac tggcggtaga accacacgcg gtcggtgtcc aggccggcag cgatccaggt
      901 ggcggcgatc tccagcgtcg agcgctgcac gcgcagcggg tcggtggtct tgatcagcgc
      961 gtggtagtcg gccaggaagt agaagctctc gacgccgggc tggcggctgg cggcgactgc
     1021 cggacggatg gcgccggcgt agttgccgag gtggggcgtg cccgaggtgg tgatcccggt
     1081 gaggacgcgc tggacagaca tggatcgctc cggctcaaac tggacaggga gcgcgagttt
     1141 aacggcttcg gcgcgcgcgc ataaggcgcg ggcgcagctc gctcactcct gccagcgcag
     1201 cgacaggcgt gcgccgccct gcggcacgag atcgacccag tgttcctggc gcgcgccggc
     1261 ataggacgcg gagatccggt agcgccccgg cgcgggcacg tcgacctgac cgatcggccc
     1321 gcaggacgcc tcgacgcgct gcccggagag cggctcgtcg acccgcgtct cgacgtcggt
     1381 gacccagccg cccgcggtgg tggagaagaa cacggtcagc gcatgcatgc cgatctcggc
     1441 gcgcatgcgc tcggactcct cgagcccgat gccgccgcac tccagccgga gcacgggcgc
     1501 atcgtccacc accggctgcg tccatcccgg ggcggcgagc gcaagcaggg caagggcggc
     1561 gaacacgcgt ttgcaaggtt tcatcattgg gtgcgactcc gggaaccgcc cgaatcaggc
     1621 ttccgcattg ttcccggctc cccgcgggga ggcaaggatc ggactgcaag cgggtgacgc
     1681 agtcgcaccg cccgcgcagc atttacgtgg atcttgcgtt cctttagacg cgatctttac
     1741 caaaggtggg gaaattgtca cagtcattcg agcggatctc ggcgaccctt caggcacatc
     1801 ccgccctccc atcccgtggg gcgactgcat gcacacgacc agctcgatag agaagacgac
     1861 cgaaccgatg aaaaccctgc tcctcgccct ggccctgtcc gccgtgggca cggctcaagc
     1921 cgccgtatgc caactcagcg acgtgaaggt gaccggcgtc agcatctaca gcggaacgac
     1981 gcgggacgcg gcgcttgccc cgatcccgat cgacaccctg ccggtcagcg ccgccgcctg
     2041 tgccggcgcg ttcagcggca acgacggcca ctacccccag accaatctcg gctacgcggg
     2101 cgacggactg ctcaacggcg gcgcccagat gggcagcaag tcggtcctgt tcccgggcgg
     2161 ggccttcatc agcgagcact acccgctcca ggacctgcgc ggcgacggca cgaccgacga
     2221 cccgggctgg atcatgctgg gaaagtcgga aacacagggc gggcgctggc agttctcccc
     2281 gagcgcgatc ggcggtgacc gctcggtggt cctcgactcc ttcttcagcg tgaccgaaac
     2341 cagtgccggc aagggaacgt ggtccttcac gccggatgcg gaagcggcac ggcgcgccgc
     2401 ctccgtcctt ggaggcaact ggatcgacca gttcgcgatc tcgttcaagg cggggaacgg
     2461 cttcgccctg tacagcttca cctccgcact cttcgatgct gcgacaccga aggcgagcga
     2521 cccgctgctg aactggttcg gcagctacga tctgaccgag accttgatca accgaggcgg
     2581 caacccggcg gggctgtccc acgtcacgct gtgggtgcgc gaccctgcga caagcccgga
     2641 tccggtcccc gcgaccaatg cggtgcccga acccggctcg ctgggcctgc tcgccctcgc
     2701 cctcgtcggc ctgggcttgc ggcgtcgcgg ctgaccctgg cgggtgccgc gcgggtccgg
     2761 ggcggcaccc ggcgcccccc tgcgcatcca ccccgcggat gcacgcccac cggccggcaa
     2821 gcacgcacga aatgcccggc cggccgaagc gccgtgggcg cggccggccg aacgcgttaa
     2881 cattgcgcgt ctcatgccgc atgcgcaccc gcccgcgggt acgcaccgcc cccgcaccgc
     2941 ccggcctgcc tgcctgcccc tcgtcgccag cctctcaatt ccagccgaca tcgtgaccca
     3001 gcctcttacc gaacgtcccc gcgtccccat catcaccgtg ggcctgcgcc cccaccccac
     3061 cagcccggac gccgaagcgc cctggcaagg cccggtgggc cgcctgctcg ccaccatgtt
     3121 cgccctcccc gaaggcggcg cggtgttcga gttccgcctc gaggagagcc gccccgccca
     3181 gctcgcgatc ccggcaccgg ccacgcccgg ccccgccgac gagctgtgga agcacacctg
     3241 cttcgaggtc ttcctcggcg tgccgggcga ggccggctat cgcgaataca acttctcgcc
     3301 ctcgggacag tgggcgatct atggcttccg cgcctggcgc gagcgcatcg aggactacat
     3361 ccccgcggcc aagcccgagg tcctcttcga ccagaccgac gacggcctgg tgctggaggc
     3421 ccgcgtgccc gccgagctgc tgccggcggt gccggccggc agcgacctgc aggtgagcct
     3481 ggccgcggtc gtcgaacgca aggacggcat gctcgaatac tgggcgctgc gccacgccgc
     3541 cgcgcaaccc gacttccatg cccgtgacac cttcgtgctg accctggcca ccacgacgca
     3601 gaaggcagcc tgaagccatg gcgcaaccgg tccgtttcgg cctcgaccgc ctgctggagg
     3661 attccgcgtt gcgccgcccg ctggcgggca gacgcgtcgc cctgctcgcc cacccggcct
     3721 cggtcacgcg cgcgctggtg cattcgctcg acgcgctcgc ggccctgccc gggctccagc
     3781 tgacggccgc cttcggcccc cagcacggcc tgcgcggcga caagcaggac aacatggtgg
     3841 agtcgcccga ctacaccgac ccgctgctcg gcatcccggt gttcagcctg tacggcgagg
     3901 tgcgccgccc gaccgcggcg atgatggaca gcttcgacgt gctgctggtg gacctgcagg
     3961 acctcggctg ccgcatctac accttcatca ccacgctgcg ctacgtgctc gaagaagcgg
     4021 cccgcctcgg caagtcggta tgggtactcg accgccccaa ccccgccggg cggccggtcg
     4081 aaggcacgct gctgcgcgac ggctgggaga gcttcgtcgg cgccgggccc ttgccgatgc
     4141 gccacggact caccatgggc gaactcgcgc actggttcgt cgccacgttg aagctggacg
     4201 tcgaactgcg cgtgatcgag atgcaaggct gggaccccga cgccgccccc ggcttcggct
     4261 ggccgctcgg cgagcgcagc tgggtcaacc ccagccccaa cgcccccaac ctgtggatgg
     4321 cgcgcgccta cgccggcacc gtgatgctcg agggcacgac gttgtcggaa gggcgcggca
     4381 ccactcgccc gctggagctc ttcggcgcgc ccgatgtcga cgcgcgcgcg ctcgtcacgg
     4441 agatgcgcgc gctcgcgccc gagtggctcg ccggctgcgt gctgcgcgag tgctggttcg
     4501 agcccacctt ccacaagcac gcagccaaac tgtgcaacgg cgtgcacatc cacgtcgagg
     4561 acccggcgca ctacgaccat gccgctttcc gtccctggcg ggtgcaggcg ctcgccttca
     4621 aggccatccg ccggttgtgg cccgactacc cgctgtggcg cgactttccc tacgagtacg
     4681 aacacgggcg cctcgcgatc gacctgatca acggctcgcc gctgctgcgc gagtgggtgg
     4741 acgacccggc ggcgacgccc gcgatgctcg acgcgctcgc ctgccccgac gaggcggcgt
     4801 gggtcgagac gcgccggccc ttcctgctct accccggctg aggcctcgcc gcggccagcc
     4861 gccgcgcctc ggcgagggcg gcgtcgagct cgtcgtgcag gatcgcggcg ggcagctgcg
     4921 gcgcacccag gcgcagcagc tgggcgtgga tctcgggcgg caccaccacg cgcgcggcga
     4981 tcgactgcgc ctggaggccc tcgatcattt cctccagcgc gaactgcgcc gacaggtcca
     5041 tgaagggcac ccggctgcag tcgaagacga cgacgcgggt gccgatcacc tggtccacgc
     5101 ggtcgagcag ctggctcgcc gagccgaaga agaaggcgcc ctcgatctcg atcacgcgca
     5161 cgccgctgtc gaggccgggg agctcgtcac cggcgggtgt ctgaccgccc tcctccgcat
     5221 gggggagcgg acggacgcgc aggctgctct ggcgggcgag gcggtggatg atcagcgcca
     5281 tcgacaggat cacgccggcg cccaccgcca cgatcaggtc caccagcacg gtgagcgcga
     5341 acaccgccgc catcaccgcg acgtcggcgc gcggcgccgt gcgcaccagg cgcagcatgc
     5401 ggtagtcgag gatgtccacg ccgaccttga tcaggatgcc ggcgagcacc gcgagcggga
     5461 tacgctcggc gagcggcccc agcccgagca gcagggcgag caggaagagc gaatgcacca
     5521 cgcccgaggc acggccgcgc ccgcccgact tgatgttgat gaccgtgcgc atggtcgcgc
     5581 ccgcgcccgg caggccgccg acgaaggcgc acagcaggtt gccgatgccc tggccgacga
     5641 gctcgcggtt ggagtggtga cgggtgcgcg tgatcgagtc ggcgaccagc gaggtcagca
     5701 ggctgtcgat gctgccgagc accccgaggg tgatgccgag cagcagcacg gtggtccagg
     5761 tctccagcgt gaagctcggc agcgccggta cgggcagccc ggcggggatg tcgccgatca
     5821 ccggcacctg caggtcggcg gcggcggaca gcgcgctcat cgccaccagc gcgaccaggg
     5881 gtgcaggcac gatccggctc accgtcatcg gggtgcggaa cacgatcagc agggtgagcc
     5941 cgccgagcag cagggcctcg gcgttcacct tggcgagcac gcccgggagc gcgagcaccg
     6001 cggccagcgg cgaggacgcc ggcgccacgc cgagcagcgg cgcgctctgc agcagcacga
     6061 tgatcacgcc gatgccgctc atgaagccgg agaccaccgg ataggggatg aagcgcacca
     6121 gcccgccggt gcgcagcacg ccgaggccga tctgcaccaa cccgcccacc agcaccgccg
     6181 ccatcgccag cgcggcatcg ccgcccacca ccgcgagcgc ggaggcgaac accacggtca
     6241 tcggcccggt agggccggag atctgcatgc gggtgccgcc gagcagcgcg gcgacgaagc
     6301 cgagcacgat cgcaccgtac agcccggccg cggcccctgc gccggaggcc acgccgaagg
     6361 cgagcgcgag cggcagcgcc acgatgccgg cggtgaggcc gccgaagaag tccccgcgca
     6421 aacgggcgag cgccgaagcc tgcgcattca tgagcgcctc gaatcgaagt ctcgtccggg
     6481 cggctcggac gggttcgtgg gtgctgtccc cgaccgccgt gttttgtaca acgatgtggc
     6541 aaaaagcggc gcgccgcaag cccccgccac ttgccctaga gcgcttcggc gaagcctcgc
     6601 ttcgggaagc attcagcttc ctgattcgcc cccggccgcc ctcttcccgt agcattgcgc
     6661 ctgcgcccga aaccgcggcc cgcgcccccg atccggcgct gcgcgaaccc accacaggag
     6721 cctccaccga tgatcaagca cggcctgcag atcttcgcct gcaacgccag ccgcgagctc
     6781 gcgtgcgaca tcagcaaccg cctcggcctg cgcccgggcc gcatcgacat ctcgcgcttc
     6841 tccaacgaca acctgcgcgt gcagatccag gagaacgtgc gcgaacgcga cgtcttcgtc
     6901 gtgcagtcct tcaccgagcc ggtgagcgag cacatcatgg agttgatgat cacgctcgac
     6961 gcgctgcgca gcgcctcggc gcagcgcatc accgcggtga tcccctacta ctcgtacgca
     7021 cgctcggaca agaaggacgc gccgcgcatc tcgatcaccg gacggctgat cgcagatctg
     7081 ctgcagaccg ccggggcgaa ccgggtgctg accatggacc tgcacgccga ccaggtgcac
     7141 ggcttcttca gcgttccggt cgaccacctc accgcggtgt cgctgatcgc cgagcacttc
     7201 cgcaaccgcc acgacctgtc gaacatggtc gcggtcgcca ccgacgccgg cggcgccaag
     7261 cgcaccgggc gcttctcgga gaagctcggc atcccgatgg cgatcatcga caagcgccgc
     7321 gtgtcggaca cggcggtcaa acagggtgcg gtggtcggcg acgtggccgg gcgcgacgtg
     7381 gtgatcttcg aggacgagat cgccaccgcc ggcaccctgg tgtcgtcggt ggacacgctg
     7441 aaggccgccg gcgcgcgcag catccacgcc gccgccaccc acggcgtgct gtgcggaccg
     7501 gcgatcgagc gcctgcgcgc ggcggcgatc gactcggtgg tggtcaccaa caccgtggcg
     7561 ctgccggaag acaagcgtct cgacaagctc gccgtgctca acgtcgcacc gctcttcgcc
     7621 gcggcgatcg agcgcatcca cagcggccag agcgtgggcg cgctgttcga gtgagcggga
     7681 gcgcgctcag ttgagcacga aatccccatc cggctgcggc gcgggcggcg gcgtgtcgcc
     7741 gagcagctcg cgcaggctgg cgtcgatgga ggcgacgatg gcatcgagcg ccaggtcgtt
     7801 cgtcatcgtg ccgaagggct cctcgatctc gtcgctgagc gcctcgagcg cgaagaaggt
     7861 gtaggacacg aaacccacca ccagcggcgt catccagccc gtcgtatcca ccaggccgaa
     7921 gggcagcagc atgcagtacg tgtacgagct gcggtgcagg atcaccgtgt aggtgaatgg
     7981 cagcggcgtg ttggcgatgc gctcgcagcc cccgagcgcg gccgacagcg cgtcgagcga
     8041 gccctccatg tgctgcgcca gcatcggcgg caggcagccc gcctcgcgcc ggtcgcgcac
     8101 ccattggccg agccacagca gcaccagcgc gggtgcgaag cgggcactcc gcacgcgtcc
     8161 gagcaccgcg tccggaagca ggccttcgag cccctcgtcg cgcggacggc cgcgcagctg
     8221 gttgcgcatc gtgcttgcga aggcgatcag gccgagcacg aaggggcggg catcgacgtc
     8281 ggcgcgcgtc agggtcaggg catgccgcga caggttgcgc gcctcgacca gcacgacgcc
     8341 ccagaatttg cgcgcttccc agtagcggtc gtagctcgcg ttgatgcgaa agccgaggaa
     8401 gatcgccagg gcgaccccca tcagcgagaa cggcgcggag gtcagcgtga ccttgacgtg
     8461 gaagagctgg ccgtgcgcga gcaccaccgc gcaggacagc aacagcacga agagctgctg
     8521 cgagaagatg cgcggcaaca aggagccgcg gcggacgaag agaaggtgaa tccagtgcgg
     8581 acgagggcgg acgatcacga cgggcgggcg cggggcgagg cgcaaaggcg tggattctga
     8641 cagcttcgcg gcgaggggga gcaatttcga tcgcaaccga cgccctcggc gtccggtccc
     8701 ggccgcgacc gacgggcctg cggtgcccgc gtcgccctca ctcgccgggc cgcgccgaca
     8761 ccagccagta ggtcacgccg agcgcgagca cgatcacgcc gatcgcgaac aggtacatcg
     8821 gctccagcgt cgacaggtcg agcacgatca ccttgcgcgc gatcgccatc agcgcggtgg
     8881 cgatcaccag cttgacgtgg atgacgtcgt cgcgcaggta gagcgtgatg ttgacgaaga
     8941 tctcgatcgc gatcagcacc gccaggaagg cggcgaagac cacgaagatg tccttgaggt
     9001 cgagcagcag gaagggcgga tcggacagct tctcgtagag cacgaggacg acgtccgcga
     9061 cggtccacag gatcaccgcc accatcagca ccgccagcac gcggatcgcc tggcggatga
     9121 tccagtgcag gcggtcgatc aggggatcgt cgacacgatc gcccagatgg ttgtcgtggg
     9181 ccttgccggg cccgtgcctc atctcctcca tgactgcctc cgtggcggtg gtggcgagcg
     9241 cgccggatca tgttccgacc cggctcgcca ccgggttcgt tcgggcgtgc cctagtgcgg
     9301 gcgcgagccc catttttcca gcgcgcgggc gagcacctcg acctcggccg aggtctcgac
     9361 cccgtcggcg ctgacgatgt cctgcatcgt gcgcaacagg gcctgctgca gcctggggtc
     9421 ggcgatgtcc tcgagcagca ggtcgagcac ctcgtcggcc accttcaggc gcacgccgtc
     9481 gaggtagtgc gcgctcagca gcaggtcgtc gcagtattcg cgcaggatgc gctcgaactc
     9541 cacgctgccg atgcccagcc tgcgggcgag gtcggcgtgc atcatcgtgt cgacctcgtt
     9601 gcggtccagg ccgccgtcgg cgagcacggt gagggcgagc aggcgggcgg ccgccggggt
     9661 gctgttcgtt tcgtagtggc gcatggcgtg gtctcctttc gtatgcggtc tctggcgtcg
     9721 ccgcgcgctc ccgggagaag caccgcagcc gacgagtgca gactaaggga gaagcctccg
     9781 ccgaaaaagt cgactacgat tagtcgatcg atcgattttt tcgacggaac tgaaaatcat
     9841 gggcagcagc gagctcaact acaagcacct gcgctatttc tggacggtgg cgcgcgtggg
     9901 cacgatcgcc gaggccgggc gggtgctcgg gctggcgccg cactcgatca gcgcccagct
     9961 cgccaccttc gagggcacgc tcggcgtcac cctgttccgc cgcagcggcc gccgcctggc
    10021 gctgaccgaa gcgggtgaac gcatcctggg ccaggccgag gagatcttcg cgctcggcga
    10081 ccagatcgtg gagacgctgc gcgacgacag cctgcgccgc agcctgccct tccgcatcgg
    10141 catcccggac tcgatgccca agtcgatggt gcatcgcctg atcgagccgg cgctgcgcct
    10201 ggagcgcccg ggccggctgg tgtgccgcga gtcgccgctg tccgagctgc tcgccgagct
    10261 cgcggtgcac cgcctcgacc tggtgatcgc cgaccgcccg atcccgcccg aggtcagcgt
    10321 gcgcggccac gagcacctgc tcggcgagag cacgctgagc gtgttcgccg cgccggcact
    10381 ggccgggcgc ctgcagggcg actttccgcg cctgctcgac ggcgcgccct tcctgctgcc
    10441 gggcgaggac gtcgcccatc gcggcgccct gctgcagtgg ttcgagcgca tgcgcatccg
    10501 cccggcgatc gtcgccgaat tcgacgacag cgcgctgatg aaggccttcg gccaggccgg
    10561 cgatggcgtg ttcgccgccc ccaccacgat cgccgactac gtgtgccggc agtacggggt
    10621 gcgcctgctc ggcgaggtgc cggatgcgcg cgcgcgcatc tatgcgatca ccaccgagcg
    10681 ccggctctcg catccggcca tgcaggcgat ccgcaacgcg gcctcgcgcg cgctgacgcc
    10741 cgcgccgcgg gcctgagggt cggccggaat ccctcgccgg ggtcgttcag ccctgctgcg
    10801 cgtccgcctg ttgctgggcg atctcggcgc gcacctggtc catgtcgagg ccgcgcacgc
    10861 cctcgatcac ctggttcagc gcgttcgcgg gcagcgcgcc ggactcgcgg aacaccatga
    10921 cgccctcgcg gatcaccgcg atggtgggga tggagcggat ctggaaggcc gcggcgagat
    10981 cctgctgggc ctcggtgtcg accttgccga aaacgatgtc ggggttggcc tcggccgccg
    11041 cctcgtaggt cggcgcgaaa ttgcggcagg gaccgcacca ggccgcccag aagtcgagga
    11101 acacgatcgg gtggtgcgcg acggtgtcgt tgaaattgtc ggcggtgagt tcgagggtgg
    11161 ccatctgcgg ctccattcaa tattagcgaa gggcgatgat acgtcgcgcg accgcatggg
    11221 tcacggagcg gtcgggcatt tcgcctagaa taaccgtttg gacccaatcg aaaaggaaat
    11281 cccatgatcc gttcccgcgc cgccgtcgcc tgggctgcca aacagccgct cgaagtcacc
    11341 gaagtggacg tcgccccgcc caaggccggc gaagtgctgg tacgcatcgt cgccaccggg
    11401 gtctgccaca ccgacgccta cacgctttcg ggcgcggatc cggaaggcat cttccccgcc
    11461 atcctcggcc acgagggcgg cggcatcatc gaggcggtgg gtgaaggcgt gagctcggtg
    11521 gcggtcggcg accacgtgat cccgctgtac acgcccgaat gcggcaagtg caagttctgc
    11581 ctgtcgggca agaccaacct ctgccaggcc atccgcgcca cccagggcaa ggggctgatg
    11641 cccgacggca gcagccgctt cagcaaggac ggcaagccga tcttccacta catgggcacc
    11701 tcgaccttct cggaatacac cgtgctgccc gagatcagcg tggcgaagat ccgcaaggac
    11761 gccccgctcg acaaggtctg cctgctcggc tgcggcgtca ccaccggcat cggcgcggtg
    11821 cgcaacaccg ccaaggtcga ggccggcgcc agcgtggcgg tgttcggcct cggcggcatc
    11881 ggcctgtcgg cgatcatcgg cgcggtcatg aacaaggccg ggcgcatcgt cgcggtcgac
    11941 atcaacccgg agaagttcga gatcgcacgc cagctcggcg ccaccgactg catcaacccg
    12001 ctcgactacg accgcccgat ccaggaggtc atcgtcgacc tcaccgacgg cggcgtggac
    12061 tactccttcg agtgcatcgg caacgtcaag gtgatgcgct ccgcgctgga gtgctgccac
    12121 aagggctggg gcgagtcggt gatcatcggc gtggccggcg cgggcgagga gatctccacc
    12181 cgccccttcc agctcgtcac cgggcgggtg tggcgcggct cggccttcgg tggcgtgcgc
    12241 gggcgcagcg agctgcccgg ctacgtcgac atggcgcagc gcggcgaaat cccgctcgac
    12301 accttcatca cccaccgcat ggggctggag gacatcaaca aggccttcga cctgatgcac
    12361 gaaggcaaga gcatccgctc ggtgatcctg tactgatggc ggagaccgcg atgtcgaccg
    12421 cccctgagca gatcgccgcc aaccgcagct tcggcggctg gcaccgccgc ttccgccacc
    12481 gctcggcggc gctgggctgc gacatggtgt tcgcgatcta cctgcccccg caggccgcgg
    12541 ccggcccggt gccggtgctg tactggctgt cggggctcac ctgcaccgac gagaacttca
    12601 tgcagaaggc cggcgcccag cgcctggccg ccgagctggg gatcgcgatc gtggcgcccg
    12661 acaccagccc gcgtggcacc ggggtgccgg gcgacccgga cggcgcatgg gacttcgggc
    12721 tgggcgcggg cttctatgtg aacgcgaccg aggaaccctg gagccgccac taccgcatgt
    12781 acgactacgt ggtgacggaa ctgccggcgc tcgtcgaggc gaactttccg gtgagcgacg
    12841 cgcgcgcgat ctcggggcat tcgatgggcg ggcacggcgc gctggtgtgc gcgctgaaga
    12901 acccggggcg ctaccgctcg gtgtcggcct tcgcgccgat cgccaacccg agtgcctgcc
    12961 cctggggcga gaaggcgttc tcgcgctacc tcggcgcgga gcgcgcgcgc tggctcgaat
    13021 gggatgcctg cgcgctgatc gccggcgcca gcgagcgcct gccattgctg gtggaccagg
    13081 gcgaagccga cggttttctc gccgaacagc tccgccccga ggccctgcgc accgcttgcg
    13141 ccgccgccgg ccacccgctc acgctgcgcc tgcaggcggg ttacgaccac agctactact
    13201 tcatcgccag cttcatcgac gagcacctgc gccaccacgc cgccgcgctg cgcggctgaa
    13261 gcgccgtgtt cgcgccttgc cgcgcgccta cacgaagatt ctcgcgcgtc ttccgtaggc
    13321 gcgccgcaag gcgcgaagcg ggccgcggga cgagcgccct ctcacagcag cggcgccagc
    13381 cagcgtgcgg cctcgtcgac ctccatgccg gtgcggcgcg cccagtcctc gagctggtcg
    13441 cggccgatct tggagacggc gaagtagtgg ctctcggggt gggcgaagta gaagccgctc
    13501 accgccgccg ccggcgtcat cgcgtagctc tcggtgaggt ccatgccgat gcgcgcgcgc
    13561 gcgccgagca gctcgaacag cgggcccttg acggtgtggt ccgggcaggc cgggtagccc
    13621 ggcgcgggac ggatgccgcg atactcctcg cggatcaggg cctcgttgcc gagcgtctcg
    13681 tcggcggcat agccccagca ctcgcggcgc acgcgctcgt gcagccactc ggcgcaggcc
    13741 tcggcgaggc gatcggccag gctcttcagc atgatggcgt ggtagtcatc gtgcgcggcc
    13801 tcgaactctg cgacccgggc ctcgatgccg aggccggcgg tgaccgcgaa ggcgccgatc
    13861 cagtccggca cgccggattc cttgggcgcg acgaagtccg ccaggcacca gtgcggcttg
    13921 cccgccgggc gctcgtgctg ctggcgcagg ccgtgccaga ccatggcgag gtgcttgcgg
    13981 tcctcgccgg tgtagatctc gatgtcgtcc cccgccgcgt tggccgggaa cagcccgaac
    14041 accgctttgg cctgcagcca ctcctcggcg acgatcctct ccagcatcgc cttgccgtcg
    14101 gcaaagacgt tgcgcgcggt ctcgcccacc acttcgtcgt cgaggatggc ggggaagcgg
    14161 ccggcgaggt cccaggtctg gaagaa
//
LOCUS       DAOQBS010000078        14065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3394_length_14065_cov_16.031263, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000078 DAOQBS010000000
VERSION     DAOQBS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 14065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14065
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3394_length_14065_cov_16.031263"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="PKN25_09870"
     CDS             <1..975
                     /locus_tag="PKN25_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV91249.1"
                     /translation="QISIDEAFLDITGSAHLFGGPRAVAEALKARIAAETALTASIGV
                     APNKLVAKIACSLSKPNGLRVVGGDAVLGGWALFAMGLGMGAPLLAVGVASRSLLPKV
                     GPWMEGVKKAFGVMLLAVALWMITPVIPPLATMLGWAALLLFSAIFLHAIDPLPPQAK
                     GWQRFWKGVGVVLLLAGAAILVGALAGSRDPLQPLSVLRAQAAAPVDVPQFEKVDSIA
                     ELEARLATTDRPVLLDFYADWCVSCKEMERFTFSDAAVAARMSRMLLLKADVTANTDE
                     HKALLKRFGLFGPPGILFFDAAGKERAGLRVVGFMKAEPFATVLDRAL"
     gene            1034..1642
                     /locus_tag="PKN25_09875"
     CDS             1034..1642
                     /locus_tag="PKN25_09875"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNV91250.1"
                     /translation="MFSGIVAAVGRIEHVAALADGVRITVDTNGLDLSDVIVGDSVAH
                     SGVCLTVVEMDGPRLRYDVSRETLNCTVGLDAVGGEVNLEKSLRLADRLGGHLVTGHV
                     DGVGEVVKFAPIGESHELVIRAPAAIAGYIAKKGSVTVNGVSLTVNRVEGRDFWINLI
                     PHTVAVTNLKHLAPGSKVNLEIDLIARYVERMLEWKNDAAQA"
     gene            1677..2768
                     /gene="ribBA"
                     /locus_tag="PKN25_09880"
     CDS             1677..2768
                     /gene="ribBA"
                     /locus_tag="PKN25_09880"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNV91251.1"
                     /translation="MSALAPITDIIADIKAGKMVILVDEEDRENEGDLVMAAEFVTPE
                     AINFMAKHGRGLICLTLTDERCRQLGLQQMVRDNRTPHGTAFTVSIEAATGVTTGISA
                     HDRARTVQVAVARHARPEDIVTPGHIFPLTAQKGGVLIRAGHTEAGCDLAQLAGLEPA
                     AVICEILKDDGTMARLPDLVEFGQAHGLRIGAIRDLIEYRAANDNLVDKVAEKDVDTA
                     HGRFRLTAFEDKTSGDVHFALSHGDIRPERETLVRVHEPISVLDFLDAGSGHHTFPVN
                     QALATLAAAEAGVIVLLYRPMSGRELLAGLDGGHDRPTAKWDARLFGVGAQILRALHV
                     GKMRLLASPRKIPSMTGFGLEITGFVDKN"
     gene            2786..3289
                     /gene="ribH"
                     /locus_tag="PKN25_09885"
     CDS             2786..3289
                     /gene="ribH"
                     /locus_tag="PKN25_09885"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNV91252.1"
                     /translation="MQRNQSENARQGARYDGIDEIEPNFAGAGVRVGIVMARFNLDIC
                     EGLLSACTDELLALGVAREDIRIVTVPGALEIPLVLQTMARSGRHDALVALGAVVRGE
                     TYHFELVSNEMGAGVTRVGLDTGLPIANGVLTTEDDDQAVARMHEKGADCARAAVEMA
                     NLLKVVR"
     gene            3286..3786
                     /gene="nusB"
                     /locus_tag="PKN25_09890"
     CDS             3286..3786
                     /gene="nusB"
                     /locus_tag="PKN25_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNV91253.1"
                     /translation="MSDIDNTQAEGAAGAPNPTSKMARRRARELALQGVYQWLLSGNN
                     ATTVQRHLESETENLDKIDRELFVSLLRGATGAADELRGCFEPLLSRPVAELSPIEHA
                     ILLLGTHELRHNLDTPYRVVINEAIELAKGYGGTDGHKFVNGVLDKLSTRLRPEEVEA
                     ARAKRG"
     assembly_gap    4004..4103
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4107..5126)
                     /locus_tag="PKN25_09895"
     CDS             complement(4107..5126)
                     /locus_tag="PKN25_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNV91254.1"
                     /translation="MKTSSLLGLGLLAGLVGGCSLFRPAAPALAAEASPQAVAERPAD
                     PTPALDPQPPLEFRCGERRVELRHFGELATLGVDGKVFTLRPERSASGMRMVSVDDER
                     TGIWVKGNSARLSLAGVEQPECRRVAEKPLFRAVGNEPAWRLDIGAHGLELIADGGAS
                     RAFAAAPLIVEAAGMRSYQGMSAGGELEALVFERLCVDTMSGMTHPNSVEVRWQDRVL
                     RGCGGDPAQLLQGEPWAVVELDGRAVADPARMTLAFAADGRLAGLAACNRYFGSYVLS
                     GEGLRLSPLGATKMACEPRAMEDEQRFMAAAARVTGFAIAGDGGLELRAGDRVVMRAR
                     RAGGR"
     assembly_gap    5209..5308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5406..5747
                     /gene="hypA"
                     /locus_tag="PKN25_09900"
     CDS             5406..5747
                     /gene="hypA"
                     /locus_tag="PKN25_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNV91255.1"
                     /translation="MHEMSLAEGVRTLIEDAAAADGFGHVRAVVLEIGELAAVEVEAL
                     RFCLDVVLADTIAAGAAIEVETLPGAGWCPACAQEVPIHQLYDPCPHCGGYRVQTTAG
                     REMRVKALEVD"
     gene            6180..>6955
                     /gene="hypB"
                     /locus_tag="PKN25_09905"
     CDS             6180..>6955
                     /gene="hypB"
                     /locus_tag="PKN25_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNV91256.1"
                     /translation="MSPDTSSSSPVTAADTTPTSHPAAPGHHHLDFGSGPAHAHAPGL
                     SQAQMVKIERDILGKNDARAAENRAWLAARGIFALNLVSSPGSGKTSLLVRTLQLLGE
                     RVPAAVIEGDQQTEFDAERIRATGVPALQINTGKGCHLDADMVARALPRMELAADSLL
                     LIENVGNLVCPAAFDLGEAHKVAIFSVTEGEDKPLKYPDMFAAADLMLLNKTDLLPYL
                     AFDTERAIDFALRVNPRLRVIRTSATTGEGLDEWIAWIEAG"
     assembly_gap    6956..7055
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7056..7911
                     /locus_tag="PKN25_09910"
     CDS             <7056..7911
                     /locus_tag="PKN25_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNV91257.1"
                     /translation="DGEAWGGELLWVAGGEFVRLGHLRPLRLPGGDRAAREPWRMAAA
                     LLHASGRGDEIARRFPGQPAAALLAQVLARPALSPPSSSLGRHFDAAAGLLGLCEVMH
                     NEAEAAIALERLASGTAAPEVTPGDWRICDAGGALRGQADGARSRTDRGAPAAGSVPQ
                     PDDRAHPYAGAQPPGGTLELDLHPLLLRLADEPEPARGAARFQATLAAALVAWVEEAR
                     QRTGCATVVLSGGCLHNRLLADALHAALPARGFEVLGARRLSPGDAGLALGQAWVAMH
                     RLAATKED"
     gene            7914..8180
                     /locus_tag="PKN25_09915"
     CDS             7914..8180
                     /locus_tag="PKN25_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNV91258.1"
                     /translation="MCLAIPARIVELLAGDQCRVELGGVRKEISLALVDGAAVGDYVI
                     VHVGYALTRLDPEEAAQTLALIADAGIDPARALGGEHPAPGAAR"
     gene            8217..9398
                     /gene="hypD"
                     /locus_tag="PKN25_09920"
     CDS             8217..9398
                     /gene="hypD"
                     /locus_tag="PKN25_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNV91259.1"
                     /translation="MKYVDEFRDGALAAGLARALAREVDPGRDYAFMEFCGGHTHAIS
                     RYGVADLLPPNVRMIHGPGCPVCVLPIGRVDMAIGLALARPEVILCSYGDCLRVPASD
                     GLSLLKAKARGADVRMVYSAADALALAVREPAREVVFFAIGFETTTPPTALVLREAAT
                     RRLANFSVICNHVLTPPAITAILDSPAASRLGALHLDGFIGPAHVSTLIGSRPYEAFA
                     ARYRKPVVIAGFEPLDVMQAIRMLVRQVDTGRAEVENEFTRAVVRDGNPKAQAIVSEV
                     LALRDEFEWRGLGVLARSALRIRPAFAPWDAEAKFGLDYRPVPDHRACECGAILRGVK
                     KPTDCKLFGTVCTPETPMGSCMVSSEGACAAHYNYGRFRDVPVHVVGTSAPPNPDQET
                     A"
     gene            9395..10456
                     /gene="hypE"
                     /locus_tag="PKN25_09925"
     CDS             9395..10456
                     /gene="hypE"
                     /locus_tag="PKN25_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNV91260.1"
                     /translation="MNTVKTGYVRPLDLRKGRVDLGFGAGGRAMAQLISELFLRAFAN
                     DWLARGDDGAVLPAPAAGERLVMATDAHVVSPLFFPGGDIGSLSVHGTVNDLAVMGAR
                     PLYLAASFILEEGYALADLARIVESMAAAARTAGVAVVTGDTKVVEQGKGDGVFITTT
                     GVGALPAGRDPGGARARPGDVVLVSGRIGDHGMAIMAQRESLAFDSEIVSDSAALHGL
                     VEALYAAVPAEAVRVLRDPTRGGLATTLNEIAAQSGVGMELDEAAIPVSAQVQAACEL
                     LGLDPLYVANEGKLVVLCAPEHAGAALAALRAHPLGTEAARIGCATADPQNFVQLRTG
                     LGGRRMVDWIAGEQLPRIC"
     gene            10622..11428
                     /locus_tag="PKN25_09930"
     CDS             10622..11428
                     /locus_tag="PKN25_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91261.1"
                     /translation="MTIRSPLSPMLLAAALALGGCAVVPVDDYGYYEDDRYYERETVI
                     VTPPPRIEYRGLPPAAGYIWIDGYWNRVGPRPHWVPGYWSPPRMHSRPLAQPPHIDVR
                     PDGRGDRRDEHDRNRWRSDDARRDDRRWNDRSRDDVRRDRDSRPWPGFSPRGDGAGSP
                     DGPQLRDRRDLRDEARPPRIFQERPPGGREGLLRAEPPRDRAARPELRPAGAAGEARE
                     PRRSEAGGRPPEGFRGFRRDDDGEARRSFRREARDGGNAPEARGREPAGN"
     gene            11581..12378
                     /locus_tag="PKN25_09935"
     CDS             11581..12378
                     /locus_tag="PKN25_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV91262.1"
                     /translation="MKLSSLLMLSLTAGALLAGCGKNETAPAAQSAAAPAAPAKIVIG
                     LDDNFPPMGFRNEKNELVGFDIDLAKEAAKRLGIAVEFKPIDWSAKEAELNGKRVDAL
                     WNGLTITEERRKNIGFTSAYMENHQIIVVPAASAIKTKAELAGKVIGIQDGSSAVDAV
                     QKDPVAASFKELKKFGDNVTALMDLTTGRLDAVVLDEVVGRYYTAKKPNDYVVLDDHF
                     GTEEYGVGTRKDDTALLAKLQKAMDEMKADGSAARISTEWFGKNIIK"
     gene            12541..13191
                     /locus_tag="PKN25_09940"
     CDS             12541..13191
                     /locus_tag="PKN25_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNV91263.1"
                     /translation="MDYILSILGPLAEGSLVTLQLFLITLLLAVPLGLALALVRISRF
                     RVASQAVNAYIWLMRGTPLMLQMLFIYYALPFVPGIGIRLPDFPAAIVAFALNYAAYF
                     AEIFRAGIQSIDRGQYEGAKVLGFSYGQTMRRIVLPQVVKRILPPMSNETITLVKDTS
                     LIYVLALNDLLRAARGIVQRDFTITPFIVAAAFYLIMTLVLTWLFQRLEKRHAVYD"
     gene            13226..13978
                     /locus_tag="PKN25_09945"
     CDS             13226..13978
                     /locus_tag="PKN25_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNV91264.1"
                     /translation="MILADGIRKRFGANEVLKGVSLTLAKGEVVAVIGPSGSGKSTFL
                     RCLNHLETIDAGRIVIEGDVLADNDTGGRARYVADAEVRRICARMGMVFQHFNLFPHL
                     TVLENVIEAPITVKGLKREAILPKAEALLRKVGLFDKRDAYPSRLSGGQKQRVAIARA
                     LAMEPDIMLFDEPTSALDPELTGEVLRTMRQLADEHMTMLVVTHEMGFAREVASRVVF
                     MDGGELIESRPAAEFFANPQHPRTRAFLDSML"
BASE COUNT         2045 a         5222 c         4632 g         1866 t
ORIGIN      
        1 cagatttcca tcgacgaagc atttctcgac atcacgggct ccgcgcacct gttcggtggc
       61 ccgcgtgcgg tcgctgaagc gctgaaggcc cgcatcgcgg ccgagacggc gttgaccgcc
      121 agcatcgggg tcgcgcccaa caagctggtc gccaagatcg cctgctcgct gagcaagccg
      181 aatggcttgc gcgtcgttgg cggcgacgcc gtgctcggtg gctgggcgct gttcgcgatg
      241 gggctgggca tgggcgcgcc gctgctggcg gtgggcgtgg cctcgcgcag cctgctgccc
      301 aaggtcgggc cgtggatgga aggcgtcaag aaggccttcg gcgtgatgct gctcgcggtt
      361 gcgctgtgga tgatcacccc ggtgatcccg cccctggcca ccatgctggg ctgggccgcg
      421 ctgctgctgt tctcggcgat cttcctgcac gcgatcgacc cgctgccgcc gcaggccaag
      481 ggctggcagc gcttctggaa gggcgtgggc gtggtgctgt tgttggccgg cgcggcgatc
      541 ctggtgggcg cgctcgccgg ctcgcgcgac ccgctgcagc ccttgtcggt gctgcgcgcg
      601 caggccgcgg cgccggtcga tgtgccgcag ttcgagaagg tggactccat cgccgaactc
      661 gaggcccggc tcgccaccac cgatcgcccg gtgctgctcg acttctatgc cgactggtgc
      721 gtgtcgtgca aggagatgga gcgcttcacc ttcagcgacg ccgcggtcgc cgcgcgcatg
      781 agccgcatgc tgctgctgaa ggccgacgtc accgccaaca ccgacgagca caaggcgctg
      841 ctcaagcgct tcggcctctt cggcccgccc ggcatcctct tcttcgacgc cgcgggcaag
      901 gaacgcgcgg gcctgcgcgt ggtgggcttc atgaaggcgg agccattcgc gacggtgctg
      961 gatcgggcgc tctgagcgag gccggggttt ttcccctaca atcccgcctt tccccttacg
     1021 gttcaaaccc ttcatgttct ccggcatcgt cgcggccgtc ggccgcatcg aacacgtcgc
     1081 ggcgctcgcg gacggcgtgc gcatcaccgt cgacaccaac ggcctcgatc tttccgacgt
     1141 gatcgtcggc gacagcgtgg cgcacagcgg cgtgtgcctc accgtggtcg agatggacgg
     1201 cccgcggctg cgctacgacg tctcgcgcga gacgctcaac tgcaccgtgg ggctggacgc
     1261 cgtcggcggc gaggtcaacc tcgagaagtc gctgcgcctg gccgaccgcc tcggcggcca
     1321 tctcgtcacc ggccatgtgg acggcgtcgg cgaggtggtg aagttcgcgc cgatcggcga
     1381 gagccacgag ctggtgatcc gcgccccggc ggcgatcgcc ggctacatcg cgaagaaggg
     1441 ctcggtcacc gtcaatggcg tcagcctcac ggtcaatcgc gtcgagggcc gcgacttctg
     1501 gatcaacctg atcccgcaca cggtcgcggt caccaacctc aagcacctcg cgccgggcag
     1561 caaggtcaat ctcgagatcg acctcatcgc acgctacgtc gagcgcatgc tcgagtggaa
     1621 gaacgacgcc gcgcaggcct gacccgcgcg ccccaacgca acacaaagga agcagcatga
     1681 gcgcactcgc gccgatcacc gacatcatcg ccgacatcaa ggccgggaaa atggtcatcc
     1741 tggtcgacga ggaagaccgc gagaacgagg gcgacctcgt catggcggcc gaattcgtca
     1801 cccccgaggc gatcaacttc atggccaagc acggccgcgg gctgatctgc ctgacgctca
     1861 ccgacgagcg ctgccgccag ctcggcctgc agcagatggt gcgcgacaac cgcaccccgc
     1921 acggcaccgc cttcaccgtg tcgatcgagg ccgccaccgg cgtcaccacc ggcatctcgg
     1981 cgcacgaccg cgcgcgcacc gtgcaggtcg cggtcgcccg ccacgccagg ccggaagaca
     2041 tcgtcacccc gggccacatc ttcccgctca ccgcgcagaa gggcggcgtg ctgatccgcg
     2101 ccggccacac cgaggccggc tgcgacctcg cccagctcgc cgggctcgag cccgccgcgg
     2161 tgatctgcga gatcctcaag gacgacggca ccatggcgcg cctgcccgac ctcgtcgagt
     2221 tcggccaggc gcacgggctc aggatcggcg ccatccgcga cctcatcgag taccgcgccg
     2281 ccaacgacaa cctggtcgac aaggtcgccg agaaggatgt ggacaccgcc cacggccgct
     2341 tccgcctcac cgccttcgag gacaagacct cgggcgacgt gcatttcgcc ctgtcgcacg
     2401 gcgacatccg ccccgagcgc gagaccctcg tgcgggtgca cgagccgatc tcggtgctcg
     2461 acttcctcga cgccggcagc ggccaccata ccttcccggt caatcaggcg ctcgcgaccc
     2521 tggccgcggc cgaggccggc gtgatcgtgc tgctctaccg cccgatgtcc ggccgcgagc
     2581 tgctcgccgg cctcgacggc ggccacgacc gccccaccgc gaagtgggac gcgcgcctgt
     2641 tcggcgtcgg cgcgcagatc ctgcgcgcgc tgcacgtcgg caagatgcgc ctgctcgcca
     2701 gcccgcgcaa gatcccgagc atgaccggct tcggtctcga gatcaccggc ttcgtcgaca
     2761 agaactgacc acgagatccc ccagcatgca acgcaaccaa tccgagaacg cccgccaggg
     2821 cgcccgttac gacggcatcg acgagatcga gcccaacttc gccggcgccg gcgtgcgcgt
     2881 cggcatcgtg atggcgcgct tcaacctcga catctgcgag ggcctgctgt cggcctgcac
     2941 cgacgagctg ctcgccctcg gcgtcgcgcg cgaggacatc cgcatcgtca ccgtgcccgg
     3001 cgccctggag atcccgctcg tgctgcagac catggccagg agcggccgcc acgacgccct
     3061 ggtcgcgctc ggcgcggtgg tccgcggcga gacctaccac ttcgagctcg tctccaacga
     3121 gatgggcgcg ggcgtcaccc gcgtcggcct cgacaccggg ctgccgatcg ccaacggcgt
     3181 gctcaccacc gaggacgacg accaggcggt cgcgcgcatg cacgagaagg gcgccgactg
     3241 cgcccgcgcc gcggtcgaga tggccaacct gctgaaggtg gtgcgatgag cgacatcgac
     3301 aacacgcaag ccgaaggcgc cgccggcgcg cccaacccca ccagcaagat ggcccgccgc
     3361 cgcgcgcgcg agctggcgct gcagggggtg taccagtggc tgctgtcggg caacaacgcc
     3421 accaccgtgc agcgccacct cgagtccgag accgagaacc tcgacaagat cgaccgcgag
     3481 ctgttcgtga gcctgctgcg cggcgccacc ggcgcggccg acgagctgcg cggctgcttc
     3541 gagccgctgc tgtcgcgccc ggtcgccgag ctgtcgccga tcgagcacgc gatcctgctg
     3601 ctcggcaccc acgagctgcg ccacaacctc gatacgccct accgcgtggt gatcaacgag
     3661 gccatcgagc tcgccaaggg ctacggcggc accgacggcc acaagttcgt gaacggcgtg
     3721 ctcgacaagc tctccacccg tctgcgcccg gaagaggtcg aggcggcgcg cgccaagcgc
     3781 ggctgaacgc gttcgcattc gcgcgcgagc ggggccgcac ggcacgtgca tagcccgcgc
     3841 acggccccgg cgccaaagat cgcgacttgc gtcgctccta cggaaaaccg gcacagcacc
     3901 tgaagccccc gcaggagcga cgcaagtcgc gatcacggcg ccccaccgac cgccacatcg
     3961 accggagccg cggatcgcgg cgcgagcccg acccaccccc gacnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnccctcac cgcccgcccg cgcgccgcgc gcgcatcacc
     4141 acccgatcgc ccgcacgcag ctccagcccg ccgtcacctg cgatcgcaaa gcccgtcacc
     4201 cgcgcggcgg ccgccatgaa gcgctgctcg tcctccatcg cacgcggctc gcaagccatc
     4261 ttcgtcgccc ccagcggcga cagccgcagg ccctcgcccg acagcacata gctgccgaag
     4321 tagcggttgc atgcggccag gccggcaagc ctgccgtcgg ccgcgaaggc gagtgtcatc
     4381 cgcgccggat cggccaccgc gcggccgtcg agctccacca ccgcccaggg ctcaccctgc
     4441 agcagttgcg cgggatcgcc accgcagccg cgcagcacgc ggtcctgcca gcgcacctcg
     4501 acgctgttcg ggtgcgtcat cccgctcatc gtgtcgacac acaggcgctc gaacaccagc
     4561 gcctccagct cgccgccggc gctcatgccc tggtagctgc gcatgcccgc cgcctccacg
     4621 atcagcggcg cagcggcgaa cgcacgcgag gcgccgccgt cggcgatcag ctccaggccg
     4681 tgcgcgccga tgtcgagccg ccacgccggc tcgttcccca ccgcgcggaa cagcggcttc
     4741 tccgccaccc gccggcactc cggctgctcg acgccggcga ggctcagccg cgcagagttg
     4801 cccttgaccc agatgccggt acgctcgtcg tcgaccgaca ccatccgcat gcccgaggcg
     4861 ctgcgctcgg gccgcagcgt gaacaccttc ccgtcgacgc cgagggtggc gagctcgccg
     4921 aagtggcgca gctcgaccct gcgctcgccg cagcggaact cgagcggcgg ctggggatcg
     4981 agcgccggcg tggggtcggc cgggcgctcg gccacggcct ggggggatgc ttcggcggcc
     5041 agcgcaggcg cggcagggcg gaacaggctg cagccgccga ccaggccggc gaggagaccg
     5101 aggcccaaca gggaagaggt tttcatcgcg agactcgagg acagggaaag acggtccgtc
     5161 attctagggg tccgccgccc cctgtgcctt ccctcgcctt ccctcgccnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gcccgccggc tgcccccggc gctttgcgca
     5341 gtccgccttc gcgtttcgct tctctccgcc gcctggggta agctcgaaga ccgaggagag
     5401 tcacgatgca tgagatgtcc ctggccgagg gtgtgcgcac cctcatcgag gacgccgcgg
     5461 cggccgacgg ctttggccac gtgcgcgcgg tcgtgctgga gatcggggaa ctcgccgcgg
     5521 tcgaggtcga ggcgctgcgc ttctgcctgg acgtggtgct ggccgacacc atcgccgccg
     5581 gcgccgcaat cgaggtcgaa accctgcccg gcgccggctg gtgccccgct tgcgcgcaag
     5641 aggtgccgat ccaccagctc tacgacccct gcccgcactg cggcggctac cgcgtgcaga
     5701 ccactgccgg ccgggagatg cgcgtgaagg cgctggaggt ggactgaaga tgggccggcg
     5761 ctcgaaggcc agacgcggct ccggtcacgc aaggcggcgc cccgcccgga cccccgatgt
     5821 ttacacgggg ctccagtacg cgatggttgc accgggctcc ggtgcgcgat ggttttttgt
     5881 aggagcgacg caagtcgcga tccagccgca cgggcaagcg cctgcgcccg ccccctcgcc
     5941 gcaatcgcga cttgcgtcgc tcctgcgtcc acctgaacgg ccgcggtcgg gtcaggggcg
     6001 tgatccttac aggcagcaag gagaaacgag agatgtgcac gacatgtggt tgcggcagcg
     6061 gtgaaacccg gatcggcgcg ctcggtggcg ggcgccccgc gggtggcgca cacccgcggc
     6121 ccggcgcgac gcgcacatgg cgtgccgtcg gcgggcacgc tgccaccccg ccccgcggct
     6181 tgtcgcccga cacgtcgtca tcctcgcccg ttaccgccgc ggacacgacg cccacctcgc
     6241 accccgccgc gcccggccac caccatctcg acttcggcag cggtccggcg cacgcccacg
     6301 cgcccggcct cagccaggcg cagatggtga agatcgagcg cgacatcctc ggcaagaacg
     6361 acgcgcgcgc cgcggagaac cgcgcctggc tggccgcgcg cggcatcttc gcgctcaacc
     6421 tcgtgtccag cccgggttcg ggcaagacct cgctgctggt gcgaaccctg cagctgctcg
     6481 gcgagcgcgt gcccgccgcg gtgatcgagg gcgaccagca gaccgagttc gacgccgagc
     6541 gcatccgcgc caccggcgtg ccggcgctgc agatcaacac cggcaagggc tgccacctcg
     6601 acgccgacat ggtcgcgcgc gcgctgccgc gcatggaact cgccgcggac agcctgctgc
     6661 tgatcgagaa cgtcggcaac ctggtgtgtc cggccgcgtt cgacctcggc gaggcgcaca
     6721 aggtggcgat cttctcggtc accgagggcg aggacaagcc gctcaagtat cccgacatgt
     6781 tcgccgccgc cgacctgatg ctgctcaaca agaccgacct gctgccctac ctcgccttcg
     6841 acaccgaacg cgcgatcgac ttcgcgctgc gggtgaatcc gcgcctgcgc gtgatccgga
     6901 cttcggccac caccggcgag ggcctggacg aatggatcgc ctggatcgag gccggnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgacg gcgaggcctg gggcggcgag
     7081 ctgctgtggg tggccggcgg cgagttcgtc cgcctcggcc acctgcgccc gctgcgcctg
     7141 cccggcggcg accgcgccgc gcgcgagccc tggcgcatgg ccgccgcgct gctgcacgcc
     7201 agcggccgtg gcgacgagat cgcgcgccgc ttccccgggc agccggcggc ggcgctgctc
     7261 gcccaggtgc tcgcccgccc ggcgctgagc ccgccgagtt ccagcctcgg ccgccacttc
     7321 gacgccgccg ccggcctgct cggcctgtgc gaggtcatgc acaacgaggc cgaggcggcg
     7381 atcgcgctcg agcgcctggc ctccggcacg gccgcgccgg aggtgactcc cggcgactgg
     7441 cggatttgcg acgcaggcgg tgcgctgcgc ggacaggccg atggagcgcg ctcgcgcacc
     7501 gaccgcgggg cgcccgcagc aggctccgtc ccgcagccgg acgaccgcgc gcatccctac
     7561 gccggcgcgc agccgcccgg agggacgctc gaactcgatc tccaccccct gctgctgcgc
     7621 ctcgccgacg aacccgagcc cgcgcgcggc gcggcacgct tccaggccac cctggccgcc
     7681 gcgctcgtcg cctgggtcga ggaggcacgg cagcgcaccg gctgcgccac ggtggtgctg
     7741 tcgggcggct gcctgcacaa tcgcctgctc gccgacgcgc tccacgccgc cctgccggcg
     7801 cgcggtttcg aggtgctcgg cgcgcgccgc ctctctccgg gcgacgccgg cctcgcgctc
     7861 ggccaggcct gggtcgccat gcaccggctc gctgcaacca aggaagactg aacatgtgcc
     7921 tcgccatccc cgcccgcatc gtcgaactgc tcgccggcga ccaatgccgc gtcgagctcg
     7981 gcggcgtgcg caaggagatc tcgctcgccc tggtcgacgg cgccgccgtc ggcgactacg
     8041 tgatcgtgca cgtcggctat gcgctgaccc ggctcgaccc cgaagaggcc gcacagaccc
     8101 tggcgctgat cgccgacgcc ggcatcgacc ccgcgcgcgc gctcggcggc gagcaccccg
     8161 cacccggtgc ggcgcgctga agtgcgccat cgagtgatgg aatacgaggc tgcgcgatga
     8221 aatacgtgga cgagttccgc gacggcgccc tcgccgccgg gctggcgcgc gcgctcgcgc
     8281 gcgaggtcga ccccggccgc gactacgcct tcatggagtt ctgcggcggc cacacccacg
     8341 ccatctcgcg ctacggcgtg gccgacctgc tgccacccaa cgtgcgcatg atccacggcc
     8401 ccggctgtcc ggtgtgcgtg ctgccgatcg gtcgcgtgga catggcgatc ggcctcgcgc
     8461 tggcgcgccc cgaggtgatc ctgtgcagct acggcgactg cctgcgcgtg cccgcctccg
     8521 acggcctttc gctgctgaag gccaaggcac gcggcgccga cgtgcgcatg gtgtactccg
     8581 ccgccgacgc gctcgcgctc gccgtgcgcg aacccgcacg cgaggtggtc ttcttcgcga
     8641 tcggcttcga gaccaccacg ccccccaccg cgctcgtgct gcgcgaggcc gccacgcgca
     8701 ggctcgccaa cttcagcgtg atctgcaacc acgtgctgac gccgccggcg atcaccgcga
     8761 tcctcgactc gcccgcagcc agccggctcg gggcgctgca cctggacggg ttcatcggcc
     8821 ccgcccacgt gtccaccctg atcggcagcc gcccctacga agccttcgcc gcccgctacc
     8881 gcaagcccgt ggtcatcgcc ggcttcgaac cgctcgatgt gatgcaggcg atccgcatgc
     8941 tggtgcgcca ggtcgacacg ggccgtgccg aggtcgaaaa cgagttcacc cgcgcggtgg
     9001 tgcgcgacgg caacccgaaa gcgcaggcga tcgtgtcgga ggtgctggcg ctgcgcgacg
     9061 agttcgagtg gcgcggcctc ggcgtgctgg cgcgctccgc gctgcgcatc cgccccgcct
     9121 tcgcgccctg ggacgccgag gcgaagttcg gtctcgacta ccgccccgtg cccgaccacc
     9181 gcgcctgcga atgcggcgcc atcctgcgcg gcgtgaagaa gccgaccgac tgcaagctct
     9241 tcggcaccgt gtgcaccccg gaaacgccga tgggctcgtg catggtgtcc tccgagggcg
     9301 cctgcgccgc gcactacaac tacgggcgct tccgcgacgt tccggtacat gtcgtcggca
     9361 cctccgcccc cccgaatccg gaccaggaaa ccgcatgaac accgtcaaaa ccggctacgt
     9421 ccgccccctg gacctgcgca agggccgcgt cgacctcggc ttcggcgccg gcgggcgggc
     9481 gatggcgcaa ttgatctccg aacttttcct gcgcgcgttc gccaacgact ggctcgcccg
     9541 cggcgacgac ggcgcggtgc tgcccgcgcc cgcagcgggc gagcgcctgg tgatggccac
     9601 cgacgcccac gtggtgagcc cgctgttctt ccccggcggc gacatcggca gcctgtcggt
     9661 gcatgggacg gtgaacgacc tcgcggtgat gggcgcacgc ccgctgtacc tcgccgccag
     9721 cttcatcctg gaagaaggct atgcgctcgc cgacctcgcc cgcatcgtcg aatcgatggc
     9781 cgccgcggcg cgcacggccg gggtggcggt ggtgaccggc gacaccaagg tggtcgaaca
     9841 gggcaagggc gacggcgtgt tcatcaccac caccggggtg ggggcgctgc cggccgggcg
     9901 cgatccgggc ggcgcgcggg cgcggccggg cgacgtggtg ctggtgtcgg ggcgcatcgg
     9961 cgaccatggc atggcaatca tggcgcagcg cgagtcgctc gccttcgact ccgagatcgt
    10021 ctccgacagc gcggcgctgc acggcctggt cgaggcgctc tacgccgcgg tgccggccga
    10081 ggcggtccgc gtgctgcgcg accccacgcg cggcggactg gcgaccacct tgaacgagat
    10141 cgccgcgcag tcgggcgtgg gcatggagct cgacgaggcg gcgatcccgg tgtcggcgca
    10201 ggtgcaggct gcctgcgagc tgctcgggct cgacccgctc tacgtcgcca acgagggcaa
    10261 gctggtggtg ctgtgcgctc cggagcacgc cggcgcggcg ctcgccgcgc tgcgcgcgca
    10321 tccgctcggc accgaggcgg cgcggatcgg atgcgccacc gccgacccgc agaacttcgt
    10381 gcagctgcgc acgggcctgg gcgggcggcg catggtggac tggatcgccg gcgagcagct
    10441 gccgcggatc tgttgagaga tcgcgtcgtc acaactgatt acctcgccgc ccacccggcg
    10501 cgcccccttt cgcctatcgt gcgcgtggag acagcagaga cgcggaaggg ggcggcgggc
    10561 ggcagcgccc cgcccccggc cgccccgcat gtcgcaccct tctccgtggc aggacgcaac
    10621 catgaccatc cgctcgcccc tttcgcccat gctgctcgca gccgcgctgg cactcggcgg
    10681 atgcgcggtg gtcccggtcg acgactacgg ctactacgaa gacgaccgct actacgagcg
    10741 cgagacggtg atcgtcacgc cgcccccccg catcgagtac cgcggcctgc caccggccgc
    10801 cggctacatc tggatcgatg gctactggaa ccgggtcgga ccgcgcccgc actgggtccc
    10861 gggctactgg agcccaccgc gcatgcattc gcgaccgctc gcgcagccgc cgcacatcga
    10921 tgtgcgcccg gacgggcgcg gcgaccgtcg cgacgaacac gaccgcaacc gctggcggag
    10981 cgacgacgcg cgacgggacg accggcgctg gaacgaccgc agccgcgacg atgtccgccg
    11041 cgaccgcgac agccggccct ggcccggctt ctccccccgt ggcgacggcg ctggttctcc
    11101 cgatggcccg cagctgcgcg atcgccgcga cctgcgcgac gaggcccgcc ccccgaggat
    11161 cttccaggaa cgcccgcccg gtggacgtga aggactgctg cgcgccgaac cgccgcgcga
    11221 tcgcgctgcg cgccccgagc tgcgccccgc aggtgccgca ggcgaggcgc gcgagccccg
    11281 ccgcagcgag gctggcgggc gaccgcccga gggtttccgg gggtttcgcc gcgacgacga
    11341 cggcgaggcg cgacgcagct tccggcgcga agccagggac ggcggcaacg cccccgaggc
    11401 acgcggacgc gagccggcgg gcaactgagc ggccacgcgc gcgagctctg cgggcagcgg
    11461 caaggttgct cgcctgacgc agtgcagcga gaatcacggt tttggcgccg gcaccccggc
    11521 gccctctccg tggcgggccc acccggcccg tccccgacca ccctcccggg caagaccaag
    11581 atgaaactct cctcgctgct gatgctctcc ctcaccgccg gtgcgctgct cgccggctgc
    11641 ggcaagaacg aaaccgcgcc ggccgcccaa tccgccgcag cccccgccgc cccggcgaag
    11701 atcgtcatcg gcctcgacga caacttcccg cccatgggct tccgcaacga gaagaacgaa
    11761 ctggtcggct tcgacatcga cctcgccaag gaagcggcca agcgcctcgg catcgccgtc
    11821 gagttcaagc cgatcgactg gagcgccaag gaagccgagc tcaacggcaa gcgcgtcgat
    11881 gcgctgtgga acggcctcac catcaccgag gagcgcagga agaacatcgg cttcaccagc
    11941 gcctacatgg agaaccacca gatcatcgtg gtgcccgccg cgtcggcgat caagaccaag
    12001 gccgagctcg ccggcaaggt gatcggcatc caggacggca gcagcgcggt cgacgcggtg
    12061 cagaaggacc cggtggccgc ctccttcaag gagctgaaga agttcggcga caacgtcacc
    12121 gcgctgatgg acctcaccac cggccgcctc gacgcggtgg tgctcgacga ggtcgtgggc
    12181 cgctactaca ccgccaagaa gccgaacgac tacgtggtgc tcgacgacca cttcggcacc
    12241 gaggaatacg gcgtcggcac ccgcaaggac gacaccgcgc tgctggccaa gctgcagaag
    12301 gcgatggacg agatgaaggc cgacggcagc gccgcccgca tctccaccga gtggttcggc
    12361 aagaacatca tcaagtaagc cggctgcgga gatcgcggct tgcgccgctc ctacgtcggc
    12421 cccgtgtaga agcggcgcaa gccgcgatct cctccgctcc ccccactcgg gcgccaacgc
    12481 cgcccgcgcc gacgctcccg cgcgcctcct acctgcccgc cgctcgcccg gcgtcccgcc
    12541 atggactaca tcctcagcat cctcggcccg ctcgccgagg gctcgctggt cacgctgcag
    12601 ctcttcctga tcaccctgct gctggccgtg ccgctcggcc tggcgctggc gctggtgcgc
    12661 atctcgcgct tccgcgtcgc cagccaggcg gtcaacgcct acatctggct gatgcgcggc
    12721 acgccgctga tgctgcagat gctgttcatc tactatgcgc tgcccttcgt gccggggatc
    12781 ggcatccgcc tgcccgactt cccggcggcg atcgtggcct tcgcgctcaa ctacgcggcc
    12841 tacttcgccg agatcttccg cgccggcatc cagtcgatcg accgcggcca gtacgagggc
    12901 gccaaggtgc tcggcttctc ctacggccag accatgcgcc gcatcgtgct gccgcaggtg
    12961 gtcaagcgca tcctgccgcc gatgagcaac gagaccatca ccctggtcaa ggacacctcg
    13021 ctgatctacg tgctcgcgct caacgacctg ctgcgcgccg cgcgcggcat cgtgcagcgc
    13081 gacttcacga tcaccccctt catcgtcgcc gccgccttct acctgatcat gacgctggtg
    13141 ctgacctggc tcttccagcg cctggagaaa cgccatgccg tctatgactg aggccgccgc
    13201 gatgacgggg ccgatgacga gcccgatgat cctcgccgac ggcatccgca agcgcttcgg
    13261 cgccaacgag gtgctcaagg gcgtgtcgct gacgctcgcc aagggcgagg tggtggcggt
    13321 gatcgggccc tcgggctcgg gcaagagcac cttcctgcgc tgcctcaacc atctggaaac
    13381 gatcgacgcc gggcgcatcg tcatcgaggg cgacgtcctc gccgacaacg acaccggcgg
    13441 ccgcgcgcgc tacgtcgccg acgccgaggt gcgccggatc tgtgcgcgca tgggcatggt
    13501 gttccagcac ttcaacctgt tcccccacct caccgtgctg gagaacgtga tcgaggcccc
    13561 gatcacggtg aagggcctga agcgcgaggc catcctgccc aaggccgaag cgctgctgcg
    13621 caaggtcggc ctcttcgaca agcgcgacgc ctacccctcg cgcctgtccg gcggacagaa
    13681 gcagcgcgtg gcgatcgcac gcgcgctggc gatggagccc gacatcatgc tcttcgacga
    13741 gcccacctcg gcgctcgacc ccgagctcac cggcgaggtg ctgcgcacca tgcgccagct
    13801 cgccgacgag cacatgacca tgctggtggt gacgcacgag atgggcttcg cccgcgaggt
    13861 cgccagccgg gtggtgttca tggacggcgg cgagctcatc gagtcgcgcc ccgcggccga
    13921 gttcttcgcc aacccgcagc atccacgcac gcgcgccttc ctcgacagca tgctgtagca
    13981 ggcgggcgtc ggccgacacg gcgcgggtgg aacggggaat cgcgactcgc gtcgctccca
    14041 ccgaaaaccg ggcgatgcgc ttggg
//
LOCUS       DAOQBS010000079        14019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3415_length_14019_cov_15.049341, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000079 DAOQBS010000000
VERSION     DAOQBS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 14019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14019
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3415_length_14019_cov_15.049341"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /gene="gatB"
                     /locus_tag="PKN25_09950"
     CDS             <1..655
                     /gene="gatB"
                     /locus_tag="PKN25_09950"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301670.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HNV91265.1"
                     /translation="KEDAHDYRYFPDPDLLPLMISPEWKARVQAEMPELPGAMKARFM
                     DDWGLSAYDATTLTASKEVADFYQATVSAAGAALAKPCANWVMGELAARLNKAELDVA
                     ASPVSPAQLAGLVARIADNTISNAIARKVFEALWNGEGATADEVIDKQGLRQVTDTGA
                     IEAMIDEVLAANQKSVEEFRAGKEKAFNALVGQVMKASKGKAGPAQVNALLRKKLGA"
     gene            689..1522
                     /locus_tag="PKN25_09955"
     CDS             689..1522
                     /locus_tag="PKN25_09955"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNV91266.1"
                     /translation="MCQLLGMNCNVPTDICFSFAGFRARGGLTDHHRDGWGIAFFEGR
                     GVRVFLDPAPSAHSPVAELVNRYPIRSLNVIAHIRKATQGEIRLENTHPFQRELWGRY
                     WIFAHNGNLKDFAPPLSGRFLPVGSTDSELAFCHILDTLAARFPAGKPAPAELHAALR
                     ELAREIGSRGEFNFLLSDGDWLFAHCSSRLCYIVRRAPFAEAHLADEDLTVDFRRLTG
                     PQDRVAVIATTPLTDNEHWTQIAPGNLVAFRDGDTIALGETATVAQDYPRRGIDKAPS
                     A"
     gene            1600..2019
                     /locus_tag="PKN25_09960"
     CDS             1600..2019
                     /locus_tag="PKN25_09960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNV91267.1"
                     /translation="MDCPLCALPSELVLWEDERCRVIRVDDAAHPGFCRVVWGEHVGE
                     MTDLSPADRRHLLDVVMATEAALRNLLDPDKINLASFGNMVPHLHWHVIPRYRDDRHF
                     PESVWGAPQRDGVIHPAADAVALARAIDGALHPSGPD"
     gene            2034..3653
                     /locus_tag="PKN25_09965"
     CDS             2034..3653
                     /locus_tag="PKN25_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91268.1"
                     /translation="MDYSQPAVCLALLQKLHPTDFRATHASLGRIVNGLLEASPAPNQ
                     HLEVLEAARETLAFVQGEMAHAYGIHPQPPARNQEGGALERVVSLWKAVARSYARIAD
                     ADAREGTLEDQRALLAQRRVFYEGLVLVEYYRAHLELPQGLWRAVHVAYNDAVRNGLA
                     QVRVTDPLNEVWRAQSAQEAYVATLLAELANPYGRGERELNWVVRWAQRFAPYCTLDT
                     GIEGHKPSTYGIDTDGDSGLRPLGLLARTPTVLRFDGSGLATQIQAVLAQFKRGVTPA
                     SLGLGNDCSASSSARLLLSLYRPWGLAASGRRFPRRQKAGAIDFTGDWLAIGYAIKGE
                     LFQQPVGEQRARAFRDDISLLTFGERVATVDGSTSESRRREAQAADLGMECERWELMD
                     QSVGGFRLRRSGNGARLDHHQLVGIRPPDADQLLIADLSWLMYRTDGTLEVGVNVLPG
                     VPRVVGVRRTAARTGAREPFHQAFVLPPSPALKTAGALVLPPAWFKKDRIVEVRDGEE
                     RQQVRLLKVLMRGANFDQCSFVPVEQEVRPA"
     gene            complement(3640..4956)
                     /gene="phoR"
                     /locus_tag="PKN25_09970"
     CDS             complement(3640..4956)
                     /gene="phoR"
                     /locus_tag="PKN25_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HNV91269.1"
                     /translation="MIRRPLSYVALVIVLPLGTLMVLAALAAAYRGSDAALALLVVGL
                     VGWTIMLVHNLRQLVIWARQPVGTPVPQVEGLWGRIFAEIGDRMQSECEVKERLSAEL
                     GRFQQAALAMPDGVIYLAEDDTIEWMNPMAEQHFDLDRRKDLRMALPGLVRQPEFVRY
                     LAGREYAEPLVMRSLRRRERSLQVQVIGFAGNRRMVLSRDVSQLERLETMRRDFVSNV
                     SHELRTPLTVVCGFLETLIDGLDDFGRDEVLQYLSLASEQSSRMQRLIEELLTLSALE
                     TGAPAPAEEQVDAQALVRDVVRDCELLSAGRHEVRLVLEGGGELLGSHKELRSAFSNL
                     ASNAVRYTPAGGHIEIGWRSGAGGGEFWVADDGIGIDSAHIPRLTERFYRVDRGRSRE
                     TGGTGLGLAIVKHVTTRHQGELAIESEPGRGSRFTLRFTPARVRPA"
     gene            complement(4991..5701)
                     /gene="phoB"
                     /locus_tag="PKN25_09975"
     CDS             complement(4991..5701)
                     /gene="phoB"
                     /locus_tag="PKN25_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HNV91270.1"
                     /translation="MPANILLVEDEPAIQELIAANLARAGHHVVRANDAESAQRIVRE
                     ALPDLVLLDWMLPGASGVEFARRLRADERTRDIPIIMLTARGEEQDKVTALEVGADDY
                     VTKPFSPRELVARIKAVLRRRAPQTTEDVVELGGLRLDPATHRVSAGETALALGPTEF
                     RLLHFLMTHPERVHSRAQLLDQVWGDHVFVEERTVDVHIRRLRCALEPSAHDALIQTV
                     RGSGYRFSVHTAQAAAAS"
     gene            complement(5934..6908)
                     /gene="hcrB"
                     /locus_tag="PKN25_09980"
     CDS             complement(5934..6908)
                     /gene="hcrB"
                     /locus_tag="PKN25_09980"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit beta"
                     /protein_id="HNV91271.1"
                     /translation="MNILADFRTHRPATLADAVSALAADGAVPLAAGTDLLPNLRRGL
                     GQPAVLVDLTGIGGLETISILADGSLRIGAGATLEAIAEHGLVRNAWPALAEAAAAVA
                     GPTHRAAATLGGNLCQDTRCIFYNQSEWWRAGNGYCLKYQGDKCHVIVKNDRCYATYH
                     GDVAPALMVLGARVEIAGPAGRRSLAVEELFREDGAGHLSLGRGELVVAVEVPPASGW
                     RTAYAKARIRDAVDFPLAGVAVALRRDGDRVAGLKVAITGTNSAPLRVPTDELVGGRW
                     DAAAAETLSQLVRKTSNVLKTTVTGVGYRRRVLLALAPRTVDALWQGG"
     gene            complement(6905..9214)
                     /gene="hcrA"
                     /locus_tag="PKN25_09985"
     CDS             complement(6905..9214)
                     /gene="hcrA"
                     /locus_tag="PKN25_09985"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit alpha"
                     /protein_id="HNV91272.1"
                     /translation="MNPRLPRNGTVGARTPLVDGVEKVTGKAKYTADIAAPGALVGRI
                     LRSPHAHARILSIDTAAAEALEGVFAVCTGAETPVPYGVLPIAENEYPLAREKVRYRG
                     DPVAAVAAIDEATAEKALGLIRVDYEVLPAYFTPKAARKAGAVAIHDDKPDNVLREVH
                     AEFGDVAAAFAEADLIREKTYTFAGVNHVHMELNATLAEYDPVRDMLTLNTTTQVPYY
                     VHLKVAACLQMDSARIRVIKPFLGGGFGARTETLHFEIIAALLARKARGTVRLVQTRE
                     ETFIAHRGRPWTEVKMKLGLKKDGKIAALALEATQAGGAYAGYGIITILYSGALMHGL
                     YHIPAIKHDAWRVYTNLPPSGAMRGHGTVDTRAAFEALLTEMAEELGIDSLRIRQINM
                     LPEIPYVTMYAQRVMSYGVPECLEKVKAASGWEERKGRLPKGRGLGIALSHFVSGTST
                     PKHWTGEPHATVNLKLDFDGGITLLTGAADIGQGSNTMASQVAAEVLGVRLDRIRVIS
                     ADSALTPKDNGSYSSRVTFMVGNASISAAEELKGILVKAAAKKLDAREEDIEVIDELF
                     MVSGSQDPGLTFQEVVKAAMVDSGTITVKGTFTCPTEFQGDKRIRGSAIGATMGFCYA
                     AQVVEASVDEITGKVTAHKVWVAVDVGKALNPLAVEGQTQGGVWMGMGQALSEEIVYD
                     KGRMLHGNILDYRVPTMMESPDIEVIIVESLDPNGPFGAKEASEGMLAGFLPAVQEAV
                     YEAVGVRATEFPLSPDRITELLDAKEAAA"
     gene            complement(9211..9696)
                     /gene="hcrC"
                     /locus_tag="PKN25_09990"
     CDS             complement(9211..9696)
                     /gene="hcrC"
                     /locus_tag="PKN25_09990"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit gamma"
                     /protein_id="HNV91273.1"
                     /translation="MKNILRLTLNGRLREDLVPDNMLLLDYLREAVGLTGTKQGCDGG
                     ECGACTVLVDDRPRLACSTLAHQVAGRKVETVESLSTHGTLSKLQAAFHEKLGTQCGF
                     CTPGMLMASEALLRKNPDPSREEIKTALAGNLCRCTGYVKIIESVEAAAAARLCAEEA
                     K"
     gene            complement(9693..10148)
                     /locus_tag="PKN25_09995"
     CDS             complement(9693..10148)
                     /locus_tag="PKN25_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNV91274.1"
                     /translation="MADELGFHLRCAQLAAFKHFAHAVGAVEGITPGLYGMLQAIDNN
                     PGLSQSALAVAMDVDRSSIVKVVDRLEGKGLIVRDASPTDRRRYRLHMTATGARALRR
                     IQDAVIRQDRVFSARLDDTERATLIALLTRLYRPDSETPTARAGIGAES"
     gene            10338..11315
                     /gene="cobS"
                     /locus_tag="PKN25_10000"
     CDS             10338..11315
                     /gene="cobS"
                     /locus_tag="PKN25_10000"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HNV91275.1"
                     /translation="MTAAGPLPPLPDTECPVREAFGLDSDMKVPAFSRGSEHVPPRDD
                     TYRFDPDTTLAILAGFAFNRRVMIQGYHGTGKSTHIEQVAARLNWPCVRINLDSHVSR
                     IDLIGKDAIVLRDGKQVTEFREGILPWALQHACALVFDEYDAGRPDVMFVIQRVLEVE
                     GRLTLLDQSRVIHPHPAFRLFATANTIGLGDTTGLYHGTQQINQAQMDRWNIVATLNY
                     LDHDAEVEIVVAKMPAYDTKAGRARVGAMVMLAGLTRSGFIDGDLSTVMSPRTVLTWA
                     ENALIFADLRFAFRVSFLNRCDEIERAVVAEYYQRCFGEELIDPRAAAG"
     gene            11326..13194
                     /locus_tag="PKN25_10005"
     CDS             11326..13194
                     /locus_tag="PKN25_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HNV91276.1"
                     /translation="MTSERPRSRPLRGVANTAVFLRAVDSALRAIAGCAGGASAPSGV
                     DGIETAALPPALTTAEIVRLRGEADALALRLRHHDERLHALHDLQGERARAAYDAIAQ
                     ARVEALGAGRMPGVAANIAALIEQRCRDRRLDRATSREQVALAEALHVLARERFCGAP
                     PPPAARTMAELWRPYFDAHVGPRLDGLREHLRDERAFAAGLHELIEALDLENPTSEAR
                     EPRKLARSEGEPGDEDAAEQARAGGAAARDAQPSLRHASSGRNEGGRAAQADLAAAPA
                     RTGAAGGDAVRDAASARGPSASYRAYTTAFDQTVGAEELCDASELHPLRARLDRELMR
                     LPELVGRLAKRLQRRLLASQLRDWAFDLEEGWLDPGRLDRIVVSPDHGLSFRMEKASG
                     FRDTVVGVLIDNSSSMRGRPLAVAAMSADILARTLERCGIKVEILGFTTRTWKGGRAR
                     EQWRREGEPVAPGRLAELRHIVYKAADTPWRRARKNLGLMLRDGIPKENIDGEALLWA
                     HRRLVARPEQRRILVVVSDGAPADEATLAANPGDYLERHLHEVIAWIERRSPVELLAI
                     GIGHDVTRHYRRAVTLRDADELGAALLEQLARLFDERPRAPAGARMRPRASETAPR"
     gene            13234..13656
                     /locus_tag="PKN25_10010"
     CDS             13234..13656
                     /locus_tag="PKN25_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNV91277.1"
                     /translation="MAGLDENTPTPTDIIVHRDRKTLEVAFDDGSRFELPFEFLRVYS
                     PSAEVRGHGPGQETLQQGKRDVDIVDLAPVGNYAIKPTFSDGHDSGLYSWDYLFAIGR
                     NQEELWNNYLEALERAGGTRDPSKVPPPPPKGGCGHHH"
     gene            13664..>14019
                     /locus_tag="PKN25_10015"
     CDS             13664..>14019
                     /locus_tag="PKN25_10015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV91278.1"
                     /translation="MSDKTTHFGFQTVAEEQKEKKVAEVFSSVAQKYDVMNDLMSFGL
                     HRLWKHFTIQVSDVREGDRVLDVAGGTADLSLAFAKKVGRSGQVWLTDINHAMLARGR
                     DRMIDHGFAMPAAQCNG"
BASE COUNT         2142 a         4941 c         4817 g         2119 t
ORIGIN      
        1 caaggaagac gcccacgact accgctactt ccccgacccc gatctgctgc cgctgatgat
       61 ttcgcccgaa tggaaggcgc gcgtgcaggc ggagatgccc gagctgcccg gggcgatgaa
      121 ggcgcgcttc atggacgact gggggctgtc ggcctacgac gcgaccacgc tcaccgcctc
      181 caaggaagtc gccgacttct accaggccac ggtgtcggcg gccggggccg cgctcgccaa
      241 gccttgcgcc aactgggtca tgggcgaact cgccgcccgc ctcaacaagg ccgagctcga
      301 cgtcgccgcc tcgccggtgt cgccggcgca gctcgccggc ctggtggcgc gcatcgccga
      361 caacaccatc tccaacgcca tcgccaggaa ggtgttcgag gcgctgtgga acggcgaggg
      421 tgcgacggcg gacgaagtca tcgacaagca aggcctcagg caggtgacgg acaccggcgc
      481 gatcgaggcc atgatcgacg aggtgctggc ggccaaccag aaatcggtgg aggaattccg
      541 cgccggcaag gagaaggcct tcaacgccct ggtcggccag gtcatgaagg cgagcaaggg
      601 caaggccggc cctgcccagg tcaacgcgct gctgaggaag aagctcggcg cctgagcggg
      661 aaagcggccg cgaacggagg gtttcacgat gtgccagttg ctgggcatga actgcaacgt
      721 gccgaccgac atctgtttct cgttcgcggg cttccgcgcg cgcggcgggc tcaccgacca
      781 ccaccgcgac ggctggggga tcgccttctt cgagggccgc ggcgtgcgcg tcttcctcga
      841 cccggcgccg agcgcgcatt cgccggtggc ggagctggtg aatcgctacc cgatccgctc
      901 gctcaacgtc atcgcccaca tccgcaaggc cacccagggc gagatccggc tcgagaacac
      961 ccaccccttc cagcgcgagc tgtggggacg ctactggatc ttcgcccaca acggcaacct
     1021 gaaggacttc gcgccgccgc tctcggggcg ctttctgccg gtcggcagca ccgactcgga
     1081 gctcgccttc tgccacatcc tcgacacgct ggccgcgcgc tttcccgcgg gcaagcctgc
     1141 gcccgcggaa ctgcacgcgg cgctgcgcga gctcgcgcgc gagatcggca gccgcggcga
     1201 gttcaacttc ctgctctcgg acggcgactg gctgttcgcg cactgctcgt cgcgcttgtg
     1261 ctacatcgtc cggcgcgcgc ccttcgccga ggcccacctg gccgacgagg acctcacggt
     1321 ggatttccgc cgcctcaccg gtccgcagga ccgcgtcgcg gtgatcgcca ccacgccgct
     1381 caccgataac gagcactgga cgcaaatcgc gccgggcaac ctggtcgcct tccgcgacgg
     1441 cgacacgatc gcgctcgggg aaaccgccac cgtggcgcag gattacccgc gccggggcat
     1501 cgacaaggct ccgagtgcgt aacagttcct cgcccgcccg cagcgtcggg cctagattga
     1561 aagtccgtcg aaccctccgc agccgaccga gaaaccctca tggattgccc cctgtgcgcc
     1621 ctgccgtccg aactcgtcct ctgggaagac gagcgctgcc gcgtcatccg cgtcgatgac
     1681 gccgcccacc cgggcttctg ccgggtggtg tggggcgagc acgtcggcga gatgaccgac
     1741 ctctcgccgg ccgatcgccg tcatcttctg gatgtggtga tggccaccga ggcggcgctg
     1801 cgcaatctgc tcgacccgga caagatcaac ctggccagct tcggcaacat ggtgccgcac
     1861 ctgcactggc acgtgatccc gcgctaccgc gacgaccgcc acttccccga gtcggtgtgg
     1921 ggcgcaccgc agcgcgacgg cgtgatccac cccgccgccg acgcggtcgc cctcgcacgc
     1981 gcgatcgacg gcgcgctgca cccgagcgga ccggactgag gacgcgcgaa gccatggact
     2041 acagccagcc cgcagtctgc ctcgccctgc tgcagaagct gcacccgacc gatttccgcg
     2101 ccacgcatgc cagcctgggt cgcatcgtca acggcctgct ggaggcctcc ccggcgccca
     2161 accagcacct cgaggtgctg gaggccgcgc gcgagacgct cgccttcgtg cagggcgaga
     2221 tggcgcacgc ttatgggatc cacccgcaac ctccggcgcg caaccaggag ggcggtgcgc
     2281 tcgagcgcgt ggtctcgctg tggaaggcgg tggcgcgctc ctacgcacgc atcgccgacg
     2341 ccgacgcgcg cgaaggcacg ctcgaggacc agcgtgcgct gctcgcccag cgccgggtgt
     2401 tctacgaggg cctcgtgctg gtggagtatt accgcgccca cctcgagttg ccgcaaggtt
     2461 tgtggcgggc ggtgcatgtc gcctacaacg atgccgtgcg caacggactc gcacaggtac
     2521 gcgtgaccga cccgctcaac gaggtctggc gtgcacagag tgcccaggaa gcctacgtcg
     2581 ccaccctgct cgccgagctc gccaacccct atggccgcgg cgagcgcgaa ctgaactggg
     2641 tcgtgcgctg ggcgcagcgc tttgcgccct attgcacgct cgacaccggc atcgagggac
     2701 acaagcccag cacctacggc atcgacaccg acggcgacag cggcctgcgc ccccttgggc
     2761 tgctcgcgcg cacgcctacc gtgctgcgct tcgacggcag cgggctcgcc acccagatcc
     2821 aggccgtcct cgcgcagttc aagcgtggtg tcacgcccgc ctcgctcggg ctcggcaacg
     2881 actgctcggc cagttccagc gcgcgcctgc tgctgtcgct ctaccgcccg tggggactgg
     2941 cggcttccgg gcggcgcttc ccgcgccggc agaaagccgg cgcgatcgac ttcaccggcg
     3001 actggctcgc gatcggctac gcgatcaagg gcgagctctt ccagcaaccc gtcggcgagc
     3061 aacgggcgcg cgccttccgc gatgacatct ccctgctcac cttcggcgag cgcgtggcca
     3121 cggtcgatgg cagcacctcg gagagccggc gacgcgaggc gcaggccgcg gacctgggca
     3181 tggagtgcga gcgctgggaa ctgatggacc agtcggtggg cggcttccgc ctgcgccgca
     3241 gcggcaacgg ggcgcgcctg gaccaccacc agctcgtcgg catccgcccg cccgacgccg
     3301 atcagctgct gatcgccgat ctgagctggc tgatgtaccg caccgacggc acgctggaag
     3361 tcggcgtcaa cgtgctgccc ggcgtaccgc gggtggtcgg ggtgcggcgc acggctgcac
     3421 gcacgggagc gcgcgaaccc ttccaccagg ccttcgtgct gccgccctcg cccgcgctca
     3481 agaccgcagg cgcgctcgtg ctgccgcccg cctggttcaa gaaggaccgc atcgtcgagg
     3541 tacgggacgg cgaggagcgt cagcaggtgc gcctgctcaa ggtgctgatg cgtggcgcga
     3601 acttcgacca gtgcagcttc gtgccggtcg agcaggaagt caggccggcc tgacccgtgc
     3661 cggcgtgaag cgcagcgtga agcggctgcc ccgccccggt tcgctctcga tggcgagttc
     3721 gccctggtgg cgcgtggtga cgtgcttgac gatcgccagc cccagccctg tgcccccggt
     3781 ctcgcgcgac cgcccgcgat ccacccggta aaagcgttcg gtcaggcgcg ggatgtgcgc
     3841 cgagtcgatg ccgatgccgt cgtccgcgac ccagaattcg ccaccacccg caccgctgcg
     3901 ccagccgatc tcgatgtggc cgccggccgg cgtgtagcgc accgcgttgc tggccaggtt
     3961 ggagaaggcg ctgcgcaatt ccttgtggct gccgaggagt tcgccgccac cctcgagcac
     4021 caggcgcacc tcgtgacggc ccgcggagag cagttcgcaa tcgcgcacga cgtcgcgcac
     4081 cagcgcctgg gcatccacct gctcttccgc cggcgcgggc gccccggtct cgagcgccga
     4141 caaggtcagc aactcctcga tcaggcgctg catgcgcgag gactgttcgc tggcgaggct
     4201 caggtactgc agcacctcgt cgcgcccgaa gtcgtcgagg ccgtcgatca gggtctcgag
     4261 gaagccgcag accacggtca gcggggtccg cagctcgtgc gacacgttgg agacgaagtc
     4321 gcgccgcatg gtctccaggc gctcgagctg ggagacgtcg cgcgacagca ccatgcgccg
     4381 gttgccagcg aagccgatga cctgcacctg caggctgcgc tcgcgccggc gcagcgagcg
     4441 catcaccagc ggttcggcat actcgcgccc ggcaaggtag cggacgaact cgggctggcg
     4501 caccaggccg ggcaaggcca tgcgcaggtc cttgcggcgg tcgaggtcga agtgctgctc
     4561 ggccatcgga ttcatccact cgatggtgtc gtcctcggcg aggtagatca cgccgtcggg
     4621 catggccagc gcggcttgct ggaagcggcc gagttcggcc gagaggcgct ccttgacctc
     4681 gcactcgctc tgcatgcggt cgccgatctc ggcgaagatc cgcccccaca ggccctcgac
     4741 ctgcggcacc ggggtgccca ccggctggcg cgcccagatg acgagctggc gcaggttgtg
     4801 caccagcatg atcgtccagc cgaccagccc gaccaccagc agggcgagtg cggcatcgct
     4861 gccgcggtag gcggcggcca gggccgccag gaccatcagc gtccccagcg gcaggacgat
     4921 gacgagggcg acgtagctga gcgggcggcg gatcactggg agcggatcac gggacggatc
     4981 gggggtgcgc tcaggaggcg gccgcggctt gcgcggtgtg caccgaaaaa cgatagccgc
     5041 tgccgcgcac ggtctggatc agcgcgtcgt gcgcgctggg ttcgagcgcg cagcgcaggc
     5101 ggcggatgtg cacatccacc gtgcgctcct cgacgaagac atggtctccc cacacctggt
     5161 cgagcagctg cgcacgcgaa tgcacgcgct cgggatgggt catgaggaaa tgcagcaggc
     5221 ggaactcggt cggtccgagc gcgagcgcgg tctcgcccgc gctgacacgg tgggtggcgg
     5281 ggtccaggcg caggccgccg agctcgacca cgtcctcggt ggtctgcggc gcacgccggc
     5341 gcagcaccgc cttgatgcgc gcgaccagct cgcgcgggct gaagggcttg gtgacgtagt
     5401 cgtcggcacc gacctcgagc gcggtgacct tgtcctgctc ctcgccgcgc gcggtcagca
     5461 tgatgatcgg gatgtcgcgg gtgcgctcgt cggcacgcag ccggcgggcg aattcgacgc
     5521 ccgaggcgcc gggcagcatc cagtccagca gcaccagatc gggcagcgcc tcgcgcacga
     5581 tgcgttgcgc gctctcggcg tcgttggcgc gcaccacgtg gtggccggcg cgggcgaggt
     5641 tggccgcgat cagctcctgg atcgccggct cgtcttccac gagcaatatg ttcgctggca
     5701 tgcctgtgct ccctgaaaaa tgcccgtgaa gtgtattgca gctccttgaa gctttgatga
     5761 cacgtcatcg aagcgcaatg cacaatcccc gcgcgcctcc gtccaggccg gacaagaggc
     5821 gtgcggggcg ggcgaagcgc gccatcgtgg ggcgccacgc gggcaggatg cttgcggtcg
     5881 ccccctcgca gagggcgcgg cacagggcga acggaacacc aggctacaag gattcagcct
     5941 ccctgccaga gcgcgtcgac cgtccgcggc gccagcgcca gcagcacccg gcgccgatag
     6001 ccgacgccgg tgacggtggt cttgagcacg ttcgaggtct tgcgcaccag ctgggacagg
     6061 gtctcggcgg cggcggcatc ccagcgccct cccacgagct cgtccgtggg cacgcgcagc
     6121 ggggcggagt tggtgccggt aatcgctacc ttcaggccgg cgacccggtc tccgtcacgg
     6181 cgcagggcga cggcgacccc cgcgagcgga aaatcgacgg cgtcgcggat gcgtgccttg
     6241 gcgtaggcgg tcctccagcc gctggcgggg ggaacctcca cggccacgac cagttcgccc
     6301 cgtccgaggc tcaggtgccc cgccccgtct tcgcggaaca attcctccac cgcgaggctg
     6361 cgcctgcccg cgggccccgc gatctcgaca cgcgcgccca gcaccatcag cgccggggcg
     6421 acgtcgccgt ggtaggtggc gtagcagcga tcgttcttga cgatgacgtg gcacttgtcg
     6481 ccctggtact tgaggcagta gccgttgccg gcacgccacc actcgctctg gttgtagaag
     6541 atgcagcgcg tgtcctggca caggttgccg cccaaagtgg ccgcggcgcg gtgggtcgga
     6601 cccgcgaccg ccgcggccgc ctcggccagt gccggccagg cgttgcggac gagcccgtgc
     6661 tcggcgatgg cctccagcgt ggcgccggca ccgatgcgca ggctgccgtc ggcgaggatg
     6721 gagatcgtct ccaggccccc gattccggtc agatccacca gcacggcggg ctgtcccagg
     6781 ccgcggcgaa gattgggcag caggtcggtg ccggcagcca ggggaacggc cccgtcggcg
     6841 gccagcgcac tgaccgcgtc ggccagggtg gcggggcgat gggtgcggaa atcggcgagg
     6901 atgttcatgc tgcggcttcc ttggcgtcga gcagttcggt gatgcggtcg gggctgagcg
     6961 ggaattcggt ggcgcgcacg ccgaccgctt cgtataccgc ctcctggacg gcggggagga
     7021 agccggccag catgccttcc gaggcttcct tggcgccgaa cgggccgttg gggtcgaggc
     7081 tctcgacgat gatcacctcg atgtcggggc tctccatcat ggttgggacg cggtaatcca
     7141 ggatgttgcc atgcagcatc ctgcccttgt catagacgat ctcttccgac agcgcctggc
     7201 ccatgcccat ccacacgccg ccttgcgtct gcccttccac cgcgagcggg ttgagcgcct
     7261 tgcccacatc gaccgccacc cacaccttgt gcgcggtgac cttgccggtg atctcgtcca
     7321 ccgaggcctc gaccacctgg gcggcgtagc agaagcccat ggtggcgccg atcgcgctgc
     7381 cgcggatgcg cttgtcgccc tggaactcgg tcgggcaggt gaaggtgccc ttcacggtga
     7441 tggtgccgga gtcgaccatc gcggccttga ccacctcctg gaaggtaagc ccggggtcct
     7501 ggctgcccga caccatgaac agctcgtcga tgacctcgat gtcttcctcg cgggcgtcga
     7561 gcttcttcgc cgccgccttg accaggatgc ccttgagctc ctcggcggcg gagatcgacg
     7621 cgttgccgac catgaaggtg acgcgcgagg aatacgagcc gttgtccttg ggcgtcagcg
     7681 cgctgtcggc ggagatcacc cggatacggt cgaggcgcac gcccaggacc tcggcggcca
     7741 cctgcgaggc catggtgttg gagccctggc cgatgtcggc tgcgccggtc agcagggtga
     7801 tgccgccgtc gaagtcgagc ttgaggttga ccgtggcgtg gggttcgccc gtccagtgct
     7861 tgggcgtcga ggtccccgac acgaaatgcg acagcgcgat gccgaggccg cgccccttgg
     7921 gcagcctgcc cttgcgctcc tcccagcccg aggcggcctt gaccttctcg aggcactccg
     7981 gcacgccata gctcatcacc cgctgggcgt acatggtgac gtaggggatc tccggcagca
     8041 tgttgatctg gcggatgcgc agcgagtcga tgccgagctc ctcggccatc tcggtcagca
     8101 gcgcctcgaa ggcggcgcgg gtgtccacgg tgccgtggcc gcgcatcgcg ccgctcggcg
     8161 gcaggttggt gtacacgcgc caggcgtcgt gcttgatcgc cgggatgtgg tagagcccgt
     8221 gcatcagcgc gccgctgtag aggatggtga tgatgccgta gccggcatac gcaccgccgg
     8281 cctgggtggc ctcgagcgcg agggcggcga tcttgccgtc cttcttgagg ccgagcttca
     8341 tcttcacctc ggtccacggt cgcccgcgat gcgcgatgaa ggtttcctcg cgggtctgca
     8401 ccaggcgcac cgtgccccgg gccttgcgcg cgagcagcgc ggcgatgatc tcgaagtgca
     8461 gggtctcggt gcgggcgccg aagcccccgc ccaggaaggg cttgatcacg cggatgcggg
     8521 ccgagtccat ctgcaggcag gccgccacct tcaggtgaac gtagtagggc acctgggtgg
     8581 tggtgttcag cgtcagcatg tcgcgcaccg ggtcgtactc ggccagggtg gcgttgagct
     8641 ccatgtggac gtggttgacc ccggcgaagg tgtaggtctt ctcgcggatc aggtcggcct
     8701 cggcgaaggc cgcggcgacg tcgccgaact cggcgtgcac ctcgcgcagc acgttgtcgg
     8761 gcttgtcgtc gtggatcgcg accgcccccg ccttgcgcgc cgccttgggg gtgaaatacg
     8821 cgggcaggac ctcgtagtcg acccggatca ggccgagggc cttctccgcc gtcgcctcgt
     8881 cgatcgcggc gaccgctgcg accggatcgc cgcgatagcg caccttctcg cgcgcgagcg
     8941 ggtattcgtt ctcggcgatc ggcagcacgc cgtagggcac cggggtctcg gcaccggtgc
     9001 agaccgcgaa caccccctcc agcgcctcgg ccgccgcggt gtcgatggac aggatgcggg
     9061 cgtgggcgtg cggcgagcgc aggatgcgtc cgaccaaggc gccgggggcg gcgatgtccg
     9121 ccgtgtattt ggccttgccg gtgaccttct ccaccccgtc gaccaagggc gtgcgggcgc
     9181 cgaccgtgcc attcctgggg agcctcgggt tcatttcgcc tcctccgcgc acagccgggc
     9241 cgccgccgcg gcctccacgg actcgatgat cttcacgtac ccggtgcagc ggcacagatt
     9301 gccggccagc gccgtcttga tctcctcgcg cgagggatcg gggttcttgc gcagcaaggc
     9361 ttcggacgcc atcagcatgc ccggcgtgca gaagccgcac tgggtgccca gcttctcgtg
     9421 gaatgcggcc tgcagcttcg agagcgtgcc gtgggtcgac agcgactcga cggtctcgac
     9481 cttccggccg gcgacctggt gcgccagggt ggaacaggcg aggcgggggc gatcgtccac
     9541 caggacggtg caggcgccgc attcgccgcc atcgcagccc tgcttggtcc cggtgaggcc
     9601 gaccgcttcg cgcaggtaat ccagcagcag catgttgtcg gggacgaggt cctcacggag
     9661 acgcccgttg agggtcaggc gaaggatgtt cttcaagatt cggctcctat tcccgcgcga
     9721 gcggtcgggg tttcactgtc tggtcggtaa agtcgggtca gcagcgcgat cagcgtggcg
     9781 cgctcggtgt cgtcgaggcg cgccgagaag acccggtcct ggcgtatcac ggcgtcctgg
     9841 atacgccgta gcgcccgcgc gccggtcgcc gtcatgtgca gccggtaccg cctgcgatcg
     9901 gtcggagagg cgtcacggac gatcaagcct ttcccctcca gccgatcgac gaccttgacg
     9961 atcgaggagc ggtcgacatc catcgcgacc gccagcgcgc tttggctcag cccggggttg
    10021 ttgtcgatcg cctggagcat gccgtagagg ccgggtgtga tcccttccac cgcaccgacg
    10081 gcatgggcga agtgcttgaa cgcggcaagc tgcgcgcagc gcaggtggaa gccgagttcg
    10141 tcggccatgg gaccatggga aaccactgcc ggcttgcctg ggttcggatc atccgggggc
    10201 atgctattgg cgcctcaaat tgtttgtgtt ccaattgttg ctccaacaaa ctttttctgc
    10261 aactggattg tttgccgatt cgccaggacc gcccttgcct cctccacacc cgggagccct
    10321 cgatgaccga aatcccgatg acagccgcag gccctcttcc gcccctgccc gataccgaat
    10381 gtccagtgcg cgaggccttc gggctggaca gcgacatgaa ggtgccggcc ttcagccggg
    10441 gcagcgagca tgtgccgcca cgcgacgaca cctaccgctt cgatcccgat acgaccttgg
    10501 ccatcctggc gggcttcgcc ttcaaccggc gggtgatgat ccagggctat cacggcaccg
    10561 gaaaatcgac ccacatcgag caggtcgccg cacgcctgaa ctggccgtgc gtgcgcatca
    10621 acctcgacag ccacgtcagc cgcatcgacc tgatcggcaa ggacgccatc gtgctgcgcg
    10681 acggcaagca ggtgaccgag ttccgcgagg gcatcctgcc ctgggccctg caacatgcct
    10741 gcgcactggt cttcgacgaa tacgacgccg gccggcccga cgtgatgttc gtgatccagc
    10801 gcgtactgga ggtcgagggc aggctcacgc tgctcgatca gtcgcgggtg atccatccgc
    10861 atccggcgtt tcgcctgttc gccaccgcca acaccatcgg cctgggcgac accaccgggc
    10921 tctatcacgg cacccaacag atcaaccagg cccagatgga ccgctggaac atcgtcgcga
    10981 ccctgaacta cctcgaccac gatgccgagg tggagatcgt cgtcgccaag atgcccgcat
    11041 acgacacgaa agccggacgc gcccgcgtcg gcgccatggt gatgctggcc ggactgaccc
    11101 gcagcggctt catcgacggc gacctgtcga cggtcatgtc gccgcgcacg gtgctgacct
    11161 gggccgagaa cgcgctgatc ttcgcggacc ttcgcttcgc attccgggtc agcttcctga
    11221 accggtgcga cgagatcgag cgcgcggtgg tggccgagta ctaccagcgc tgtttcggcg
    11281 aggagctgat cgatccgcgc gccgccgcgg gctgatcgca gcctcatgac aagcgaacgc
    11341 ccccgctccc ggcccctgcg cggagtcgcg aacacggccg tctttctgcg ggcggtcgac
    11401 agcgccctgc gtgcgatcgc cgggtgcgcc ggcggcgcgt ccgccccatc cggcgtcgac
    11461 ggcatcgaaa ccgctgcact gccgcccgcc ctgacgacgg cggagatcgt ccgcctgcgc
    11521 ggcgaggccg acgcgctggc cctgcgcctg cgccatcacg acgagcgcct ccatgccctg
    11581 cacgatctgc agggcgagcg cgcacgcgcg gcctacgatg cgatcgcaca ggcacgcgtc
    11641 gaggccttgg gggcgggccg gatgccgggc gtggcggcca acatcgccgc gctcatcgag
    11701 cagcgctgcc gggacaggcg gctcgaccgc gcgaccagcc gggaacaggt tgcgctcgcc
    11761 gaggccctgc acgtcctcgc ccgcgaacgc ttctgcggcg cgcctccccc gccggcggca
    11821 cggaccatgg ccgagctgtg gcgaccctac ttcgacgccc acgtcggccc gcgcctcgac
    11881 ggcctacgcg agcacctgcg cgacgaacgc gcgttcgccg cgggattgca tgaactgatc
    11941 gaggcgctcg acctggaaaa ccccaccagc gaagcccgcg agccgcgcaa gctggcccgc
    12001 agcgagggcg aacccgggga cgaggacgct gcggagcagg cgcgggcggg cggcgcggct
    12061 gcgagggacg cgcagccctc gctccgccac gcctcgtccg gtcggaacga gggcgggcgg
    12121 gcagcgcagg cagacttggc cgccgcgccg gcacggactg gcgcggcagg aggggacgca
    12181 gtccgcgacg ccgcgtctgc gcgggggccc agcgcgtcgt accgtgccta caccaccgcc
    12241 ttcgaccaga ccgtgggcgc ggaagagctc tgcgacgcgt cggaactgca cccgctgcgc
    12301 gcccgcctgg accgcgagct gatgcgtctc ccggaactcg tcgggcgact cgccaagcgg
    12361 ttgcagcgac gcctgctggc gagccagctg cgcgactggg ccttcgatct ggaggaaggc
    12421 tggctcgatc ccgggcgact cgaccgcatc gtcgtcagtc ccgatcatgg cctgtcgttc
    12481 aggatggaga aggcttccgg ctttcgcgat accgtggtcg gcgtgctgat cgacaactcc
    12541 agctcgatgc gcggccgtcc gctcgccgtt gcggcgatga gcgccgacat cctcgcccgt
    12601 acgctggagc gctgcggcat caaggtcgag atcctcggct tcaccacgcg cacctggaag
    12661 ggcggccgtg cccgcgagca gtggcggcgc gagggcgaac ccgtcgctcc ggggcggctc
    12721 gccgagctgc gtcacatcgt atacaaggct gcggacacgc cctggcggcg tgcgcgcaag
    12781 aacctgggcc tgatgctgcg tgacggcatc cccaaggaga acatcgacgg cgaagccctg
    12841 ctgtgggccc accgccgcct cgtcgcccgc cccgagcagc gtcgcatcct ggtcgtcgtg
    12901 tcggatggcg cgcctgccga cgaggccacg cttgcggcca acccgggcga ttatctggaa
    12961 cgtcacctcc acgaggtgat cgcctggatc gagcggcgct cgcccgtcga gctgcttgcc
    13021 atcggcatcg gccacgacgt gacccgccat taccgccgcg cggtcaccct gcgggatgcc
    13081 gacgagctcg gcgccgccct gctcgagcag ctcgcgcggc tgttcgacga gcggccccga
    13141 gcgcccgcgg gagcgaggat gcgcccgcgg gccagcgaaa cggctccccg atgattcggg
    13201 tatgattggg ccccctgcac gcggaccgac gccatggccg gactcgacga aaacaccccg
    13261 acgcccaccg acatcatcgt ccatcgcgac cgcaagacgc tcgaagtcgc cttcgacgac
    13321 ggcagccgct tcgagctgcc gttcgagttc ctgcgcgtgt attccccctc ggccgaggtg
    13381 cgcgggcacg gcccgggaca ggagaccctg cagcagggca agcgcgacgt cgacatcgtg
    13441 gacctcgcgc cggtcggcaa ctatgcgatc aagcccacct tctcggacgg ccacgacagc
    13501 ggcctgtact cctgggacta cctgttcgcg atcggccgca accaggaaga actgtggaac
    13561 aactatctcg aggccctcga gcgtgcgggc ggcacccgcg acccgtccaa ggtcccgccc
    13621 ccgccgccca agggcggctg cggccaccat cactgagcag atcatgagcg acaagacgac
    13681 ccacttcggc ttccagaccg tggccgagga acagaaggaa aagaaggtcg ccgaggtgtt
    13741 ttcctcggtg gcgcagaagt acgacgtgat gaacgacctc atgtcgttcg gcctgcatcg
    13801 gctgtggaag cacttcacca tccaggtctc cgatgtgcgc gagggcgacc gcgtgctcga
    13861 cgtggcgggc ggcaccgccg acctttcgct cgcgttcgcg aagaaggtcg gacgcagcgg
    13921 ccaggtctgg ctcaccgaca tcaaccacgc gatgctcgct cgtggccgcg accgcatgat
    13981 cgaccacggc ttcgcgatgc cggccgcgca gtgcaacgg
//
LOCUS       DAOQBS010000080        13560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3600_length_13560_cov_14.590004, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000080 DAOQBS010000000
VERSION     DAOQBS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 13560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13560
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3600_length_13560_cov_14.590004"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="PKN25_10020"
     CDS             <1..1219
                     /locus_tag="PKN25_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91279.1"
                     /translation="GPNRRLVERAAKFSLDLGDYWASARETASDAYEFGASTLERFGA
                     GLEADFKSIDRGLEERLAEWEEATSPAAEYDPDRIGAAADALRESIRAARATLDSRLE
                     GMRLQDRAEIACLSLLGSALTGLSEQIAERLHAIAAGGIVEPITMSTQTKQLIERAGM
                     DEAGDLIEQARANMETELAKRRDNKARSEQLIYDRTTELDGLRIELQGPDLTAAQRDE
                     ATRKVSKLERDISRLKSVSDRLDSVIALTENGLAAASDRSAAYEQSFQTAASALANGQ
                     DLAEFWRSSKSRLFSEVDALLGKSVRKVLDAAFDSDLASKLDDFTKQLVKSKLDPQKL
                     QNLASTLLQTLDAYALRTRSILNGEDSRAAAGDPLAIEQIADGACAPIAALRAAIAKE
                     LTFLFQNGAFESR"
     gene            1226..4576
                     /locus_tag="PKN25_10025"
     CDS             1226..4576
                     /locus_tag="PKN25_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91280.1"
                     /translation="MNTTECTSPASTSETPFDPYYSEILVPAAAGDSVAMQQVLTARR
                     HVLNERTVAATTNLTVYGDEVVVEGVITLPGRQVTIVCRRLEFLPDPLNPGNYGGIDV
                     SGCRGKDGCSGGQTTAGTEPMAATAGQDGQPGRHEGGVGSNGQPGGDGGKGGEGGSIT
                     IWCDRLVRGPQLVLDASGGLGGNGGKGQPGGRGGNGIDNLDDNDSESARGGAGGCGGH
                     GGNGGAGGDGGTISLNFLGFDFPMSGMSLSVAGGIGGNGASPGIGGNGGNGGTPKMDS
                     LRYKPNFLAFMGGRDPDGHQNGADSGGGGACGLPGYGGVAGSPGTIAIGALRPPAVPS
                     VDPKTYQIIRVEAEQAAFEGTIANIWAVWTRAKVWGSWNEIHTPIDANGADGQALPAE
                     QTFGKAGTPGGPAPVDPRYGAVNGGSDIRGRGFGPGQSGQVGANNTNRPAQGARPVAT
                     PPTRVGISPASNYPGCWSIVDDRSLALQLSGDLLPMVFERCRERFLFTNVHANTNETR
                     EAVGSLQATLSWIVRMATERKEADLAAAATMTLESLRQGRNLFGHDHDFVALGSIDRY
                     QPDLTEVVAEYSAIEQTHGALAGALKNAQERAGFLGNLSGSQSRTLTALEADVADSVA
                     KVNAALTSVQQFEANRAEGSVHLIKKFTDFENEVTLGTGLTPAALLSAIGQLAFINPE
                     APAFAIPLVASQAGDLISKTANEIATDNGTTVNRSYLSRQVLSLSNEVTDFTGLKQAS
                     DGLISADGTEQGRLMATRRQVDAICAEFFSKCPTAKKLSDDLAHYIELVATRNAKIAE
                     YNDALTELCYLRSQADAIRSAKNKTESWRVSQAGLRLPQMAAFSSALWRHAREQCVET
                     LYLASRAFTMHTLSNKDVFVDVLRQLMAGSQRPDNINSTAFRAALIDFIVGRLKEENK
                     RLSTIQAFVPMGNSCRIEFTKARNPLLFATLMNGNEGLVSILPARKDTNTDHTHFPGM
                     ANVRLSAIRACALGMRTKDNIHVIQFTHPGVETIVTASDVPVTVNHQKVHHEYRYDAR
                     ALSPDGTTISAGAAAAARPETGTVTSAPAATPAPVLTGGFLDAQRQMIGPFCQWSIRI
                     PREYNSELDLSGLRSIIIEFEGTYQSFA"
     gene            4750..6597
                     /locus_tag="PKN25_10030"
     CDS             4750..6597
                     /locus_tag="PKN25_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNV91281.1"
                     /translation="MSARSTRLSAAASALHDRPPPAVEYRIRPANPGAHLFEVSCTVA
                     EPDPAGQVFSLPAWIPGSYMIREFARNIVRLRAEADGEPCALEKLDKHTWRAAAVPGA
                     RVLSVHYEVYAWDLSVRTAHLDTTHGFFNGTSVFLAVAGRTEAPCVVTIERPEGDCGR
                     DWKLVTALPPEHGHPGQACRFGRFRAADYDELIDHPVEMGRFTLARFEAAGVPHDIAL
                     TGRHDCDLERLCADLRRICEWQIALFGTPAPVDYYAFLTMVVGEGYGGLEHRASTALI
                     CSRAELPWKGMEGLPDGYKSFLGLCSHEYFHTWNVKRIKPVAFTPYDLARENHTRLLW
                     AFEGFTSYYDDLALVRSGVIGIDDYLGLLGKTIANVLRGSGRLKQSVAESSFDAWTKY
                     YRQDENAPNAIVSYYAKGALIALALDLQLRAGSEGAASLDDVMRLLWRRHGLTGVGVP
                     EDGIFAAVRDAGGERLGARLAKWLQKAVDGCEDLPLARLLRPFGVSLRAEAAGTAPVL
                     GMKLGGGSGEAKVANVYDDGPAQAAGVSAGDVLIALDGLRISSAKGLEDLLARRGAGD
                     EVELHLFRRDELMSFRAVLAAPPAERQELKLAPRADSAAAKLRRGWLGG"
     gene            complement(6697..7281)
                     /locus_tag="PKN25_10035"
     CDS             complement(6697..7281)
                     /locus_tag="PKN25_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91282.1"
                     /translation="MPEPSSSVTVSAETRARPLPGDLDRRRALFALLVCVVALPASWL
                     LFSQLERLWPEIVRLEGLGFLAAATLLGGVMAIGPLAAALGFLIAVWFGVRSVYSARS
                     LATPLLDRVIVGAGLLVWFMPALAAIAQAVRALTTGRIHFVRPPRDYLLATDPIAFWQ
                     GVGFWLIIAGLAGFLAWRYWQPKLFPAHDAAPEA"
     gene            complement(7535..9052)
                     /locus_tag="PKN25_10040"
     CDS             complement(7535..9052)
                     /locus_tag="PKN25_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HNV91283.1"
                     /translation="MLWLAILLPALSLQVFTRGVRAAPPLAILSPRGRVLAASAAASA
                     AGIEPGQRAASALALLPELRLQPHEPAREAEALAEIATWAGRFSPRISLSAADAVLLE
                     ISSCLRLFGGTAQIESALREGLAELGFTALLGCAPTPLAARWLARAAAPSALSCPPTD
                     AALPTDAAGQDWRAALDPLPLELLSEEDACDAATLELLAGLGLRTLGELRRLPPAGLA
                     RRGAQAAARALARARGELADPRPWFEAPLRFHHGIALPAPELHAEALLFAARRLFASL
                     AAWLDARHAAVDRFRLRFGHGSRPPTELEFALGRPSRDEGHFALIVRERLAMLELSAE
                     VDSITLCAESPVAADPRARDLFGDPAQAGEDAALLLARLQARLGEECVQRLDLRADHR
                     PEAAWRNTVPSAQATTARAGRDAAAGPASTRTASPPPRTTAGLRPLWLLPAPRPISAP
                     RYTLLGAAERIETGWWDGMPVRRDYYLARDADNVLCWIFESLDAPGQWFVHGYFG"
     gene            complement(9166..9984)
                     /gene="imuA"
                     /locus_tag="PKN25_10045"
     CDS             complement(9166..9984)
                     /gene="imuA"
                     /locus_tag="PKN25_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HNV91284.1"
                     /translation="MALPLQRHADASLRAVAPAATGTHGVLAGLPAGSVWQASRLAGA
                     AGAVLPTGFSALDAELPGGGWPAGALIELLADRPGVGELSLLLPLLGATPPACWIVCI
                     APPLLPYAPALANAGVPLARLLVVRPERREDLLWAARQALLSGSCACVLAWPQRIDNA
                     GLRRLQLAAEESATPLFLFRPGSVAGQPSPAVLRIALAPQPEGLQLRILKRRGPLAAA
                     PLVLALPPGASPVPKASRAGQSPRMPLSAASHPTASTTVAPVAPACKPEARRFL"
     gene            complement(10007..10615)
                     /gene="lexA"
                     /locus_tag="PKN25_10050"
     CDS             complement(10007..10615)
                     /gene="lexA"
                     /locus_tag="PKN25_10050"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNV91285.1"
                     /translation="MTARSENLTARQQEILDFIRSTVESEGRPPTRAEVCTAFGFRSP
                     NAAETHLRALAAKGAILLEEGRARGIRLAEALGLPLVGRVAAGSPILAAEHVEARYQL
                     DPALFSPRADYLLRVRGMSMRDAGILDGDLIAVHRNSEARNGQIVVARVDDDVTVKTL
                     QRKGPVVELLPANPDYTPIVVDTRREPLVIEGVMVGLIRKDH"
     gene            complement(10675..11712)
                     /locus_tag="PKN25_10055"
     CDS             complement(10675..11712)
                     /locus_tag="PKN25_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNV91286.1"
                     /translation="MPRPHPNAPAKPPAGMRAMQLERPGEALRAVHLAVPRPGPGQVL
                     LRVEACGVCRTDLHLIDGELPDPVLPMIPGHEIIGRVVELGEGVGDAAGMVPGQRIGV
                     PWLGWTCGHCGFCASQRENLCDAARFTGYQIQGGYAEYTVADARYCFPIDEALDAAEA
                     APLLCAGLIGYRALEMAGAARRIGLYGFGAAAHIITQVAVWQGREVYAFTRPGDSASQ
                     AFARRLGACWTGAGEERPPHPLDAALIFAPAGELVPAALRAVAKGGTVVCAGIHMSTI
                     PAFPYDILWGERRIVSVANLTRRDGDEFLALAPRVPVRTEVVRFALEDANEALERLRR
                     GALQGAAVLLP"
     gene            11933..12430
                     /locus_tag="PKN25_10060"
     CDS             11933..12430
                     /locus_tag="PKN25_10060"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNV91287.1"
                     /translation="MLQSGDIAPAFSLPDADMEIFDLASVAGKHNVVLYFYPRDKTPG
                     CTRQAADFSDHDDEFARYDCIVVGVSPDDCLTHAEFRDQEGLSIRLLSDSDTEVCRLY
                     NVWHEREVDGMKKMGVRRSTFVIDKQGRIRHALYDVTPRGHAAAVFELVKELEPQHAK
                     RNRQE"
     gene            12405..12893
                     /locus_tag="PKN25_10065"
     CDS             12405..12893
                     /locus_tag="PKN25_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV91288.1"
                     /translation="MQNEIVKNSVVTLKYTVRDPEGAVIDDGEHPLVYLHGGYDGIFP
                     LLEEALHGKKVGEQLQVKLQPEDAFGDYDEELILVEDAKLFPDNIEIGMSFERVSEDG
                     EEDMIYRITDIADGKVVVDGNHPLAGVALVFDVTVAEVRAASAEEITHGHVHGPGGHH
                     HH"
     gene            13065..>13560
                     /locus_tag="PKN25_10070"
     CDS             13065..>13560
                     /locus_tag="PKN25_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV91289.1"
                     /translation="MNAPLPADLPEAATAALLHVEGLGVAIARTDGEEVRPVDGVNFA
                     IAAGETFALLGESGCGKSMTALALARLLPDGGRIAAGSVRLGDEDLLALPEAAMRRVR
                     GGRIGMIFQEPATSLNPVMSVGAQIAEALAAHGLRGAAARAEACRLLAAVGIPDPERR
                     LQEFP"
BASE COUNT         2293 a         4394 c         4903 g         1970 t
ORIGIN      
        1 gggaccgaat cgcaggctgg tcgagcgggc cgccaagttc agcctggatc tcggcgacta
       61 ctgggcgagc gcacgtgaga cggcgtccga cgcctacgaa ttcggagcct ccacgctcga
      121 acgtttcggg gcaggcttgg aagcggactt caagtcgatt gatcgaggtc tggaagagcg
      181 attggccgag tgggaagagg ctaccagtcc ggcggccgaa tatgatcccg atcggatcgg
      241 cgcggcagcc gatgcccttc gagagagtat tcgcgcggcc cgcgcaacgc tcgattcgcg
      301 cctcgaggga atgcgcctgc aagatcgcgc cgaaatcgcc tgcctgtcac tgctcggcag
      361 cgcgctgacc ggcctgtctg agcagattgc cgagcgtctg catgccatcg ccgcaggcgg
      421 catcgtcgag ccgatcacga tgagcacgca gaccaagcag ttgatcgagc gcgccggcat
      481 ggatgaggcg ggtgatctga ttgaacaggc gcgggcgaac atggagacgg agctcgcgaa
      541 aaggcgcgac aacaaggccc gcagcgagca gctgatttat gatcggacga cagagctcga
      601 cggcctccgc atcgaattgc aaggtccgga cctgacggcc gctcagcgcg acgaggcgac
      661 acggaaagtg tccaagctgg aacgggacat cagccgtctc aagagcgtgt cggaccgact
      721 ggacagcgtc atcgccctga cagagaacgg acttgcagcc gcctcggacc gtagcgccgc
      781 atatgaacag agcttccaga ccgccgcaag cgcgttggcc aacgggcagg atctcgcaga
      841 gttctggcgc tcgtcgaagt cacggctgtt cagcgaggtc gacgccctgc tcgggaagag
      901 cgtccgcaag gtactcgatg ccgcgttcga ttctgatttg gcgagcaaac tggacgactt
      961 cacgaaacag ttggtgaagt ccaagctgga tccgcagaaa ctgcagaacc tcgcatcgac
     1021 gctgctgcaa accctcgacg cgtatgcgct gcgaacccgc tcgatcctga atggcgaaga
     1081 cagccgtgcg gcggcgggcg acccgctggc gattgaacag atcgcggatg gcgcctgtgc
     1141 acccatcgcg gcgctgcgcg ccgcaatcgc aaaggaattg acctttctgt tccagaacgg
     1201 cgcgttcgaa tcgaggtgaa acgtcatgaa cacgaccgaa tgcacttcgc ctgcatccac
     1261 cagcgaaacg cctttcgacc cctactattc ggagattctg gttcccgcgg cggcgggcga
     1321 ttcagtcgcg atgcagcagg tgctgacggc aaggcgtcac gtcctgaacg agaggaccgt
     1381 tgcggcgacc accaatctga cggtctatgg cgacgaggtg gtggttgaag gcgtgatcac
     1441 cttgcccgga cggcaggtaa cgatcgtctg ccggcgcctg gagttcctgc ccgacccctt
     1501 gaacccaggc aattacgggg gaatcgatgt aagcggttgt cgtggcaagg atgggtgcag
     1561 cggaggccag acgaccgccg gcacagaacc aatggcggcg accgcgggac aggacggaca
     1621 accagggcgg cacgaaggag gcgttgggtc gaacggtcaa ccgggcgggg atggtggcaa
     1681 ggggggcgag gggggctcga tcacgatctg gtgcgatcga ctggttcggg gaccgcagct
     1741 ggtcctcgat gccagtgggg gcctcggcgg caacggaggg aagggccagc ctggcggacg
     1801 cggcggaaac gggatcgaca acctggatga caacgattcg gagtcggcac gtgggggcgc
     1861 tggaggctgt ggcggtcacg gtggaaacgg cggggcaggt ggcgacggcg gaaccatttc
     1921 gctgaatttt ctggggtttg attttccgat gtcaggcatg agcctgtcgg ttgcgggtgg
     1981 catcggcggc aacggagcat cacccggaat cggcggcaac ggtggaaacg gcggtacgcc
     2041 caaaatggac tcgctgcgct acaagcccaa tttcctcgct ttcatgggcg gcagggatcc
     2101 ggatgggcac cagaacggcg ccgacagcgg cggcggcggt gcctgcggac ttccgggtta
     2161 cggcggagtt gccggcagcc ctggcacgat cgccattggc gcgctccgcc cgccggcagt
     2221 tccatccgtc gacccgaaga cgtaccagat cattcgcgtg gaggccgagc aggcggcctt
     2281 cgaaggaacg atcgcgaata tctgggcagt gtggactcgc gccaaagtgt ggggctcgtg
     2341 gaatgagatt catacgccca tcgatgcgaa cggggcagat gggcaagcgc tgccggcgga
     2401 gcagacgttc ggaaaagcgg gaacaccagg cgggcccgca cccgtagatc cccggtatgg
     2461 cgccgtcaat ggcggctcag acatccgagg gcgcggattt ggtccggggc aaagcgggca
     2521 ggtcggcgcc aacaatacca atcgccctgc gcaaggcgcg aggcccgttg cgacgccgcc
     2581 cacacgtgta ggcatcagtc ccgcgtcaaa ctatcccggc tgctggtcga tcgtcgatga
     2641 cagatcgctt gcgttgcaac tgagcgggga tcttctgccc atggtcttcg agcgttgccg
     2701 cgaacgtttt ctcttcacga acgttcatgc gaacaccaac gagacccgcg aagcggtcgg
     2761 cagcctgcag gcaaccctgt catggattgt ccggatggct acggagcgca aagaggccga
     2821 ccttgctgca gcggcgacga tgacgctgga gtcgcttcga caggggcgga acctgttcgg
     2881 gcacgatcat gatttcgtcg cgcttgggtc gatcgaccgc tatcagccgg atctgaccga
     2941 ggtggtcgca gaatacagcg ccatagagca gacgcacggc gccttggcag gcgcgctgaa
     3001 gaacgcgcag gagcgagccg gctttctcgg caacctctcg gggagtcaga gccgcacatt
     3061 gacggcgctc gaggcggacg tagccgacag cgtcgccaag gtcaatgcgg ccctgacgtc
     3121 cgttcagcag ttcgaggcga accgcgccga gggctcggtg cacttgatca agaaattcac
     3181 cgattttgaa aatgaagtca cgctcggcac aggccttacg cccgcagccc tgttgtccgc
     3241 catcggtcag ttggccttca tcaaccctga ggcgccagcc ttcgcgatac cgctcgttgc
     3301 aagccaggcc ggcgatttga tctcgaagac tgccaacgag atcgcgaccg acaacggcac
     3361 cacggtgaat cgttcgtatc tcagccgtca ggtgctgtcc ttgtccaatg aagtcaccga
     3421 cttcaccgga ttgaaacagg ccagtgacgg actgatcagc gccgacggaa ccgagcaagg
     3481 ccgcctgatg gcgacgcgca ggcaggttga tgcgatctgc gccgaattct tcagcaagtg
     3541 tccgacagcg aagaaattaa gcgacgatct ggcgcactac atcgaactgg tcgcgacgcg
     3601 gaatgccaag attgccgagt acaacgacgc gcttaccgaa ttgtgctacc tgcgatcgca
     3661 ggcggatgcc ataaggtcgg ccaagaacaa gaccgaaagt tggcgggtca gtcaggccgg
     3721 tctcagactg ccgcagatgg ccgcgttcag cagcgcgttg tggcgtcacg cgcgcgagca
     3781 gtgcgtggag acgctgtacc tggcgagccg cgcattcacg atgcacacct tgagcaacaa
     3841 ggatgtgttc gtcgacgtgc ttcggcagtt gatggctggc tcgcagcggc ccgacaacat
     3901 caactcgacc gcgttcagag ccgcgttgat cgacttcatc gtcggacgac tgaaagagga
     3961 gaacaagcgg ctttcgacca ttcaagcttt tgtccccatg ggcaactcct gccgaatcga
     4021 gttcacaaaa gcgcggaacc ccctgctgtt cgcaacgctg atgaacggca acgaggggct
     4081 ggtgtcgatt cttccggcgc gaaaggacac caacaccgac catacacact tcccgggaat
     4141 ggccaacgtc cgcctctccg ccattcgagc gtgcgcgctc gggatgagga ccaaggacaa
     4201 cattcacgtg attcagttta cgcatcccgg cgtagagacg atcgtgaccg ccagcgacgt
     4261 gccggtcacc gtcaatcatc aaaaagtgca tcacgagtac cgctacgatg cacgtgcgct
     4321 ctcccccgac ggcacgacaa tttcggcagg tgcggcggcc gcagcaagac ccgagacggg
     4381 caccgttacc agcgcaccag cggcgacacc agcgcccgtg ctgaccggcg ggttcctgga
     4441 cgcgcagcga caaatgatcg ggccgttctg tcagtggtca atccgaattc cccgggagta
     4501 caacagtgag ctcgatctga gcggactccg gtcgatcatc atcgagttcg aaggaaccta
     4561 tcagtcgttc gcgtgatgcg cacggtaatc gatccatggg gatgacgggt atccgcggat
     4621 cgcggccgtc acgccgggcg ttcgccgttg ggcgccggga gatcgctgcc cacctctgtc
     4681 gcctccccgg cgcccggcca tgcggttacg attggacctc ttccagacga gttccgccgg
     4741 agcctgccga tgtccgcccg ttccacccgc ttgtccgccg ccgcgtcggc gctccatgac
     4801 cgtccgccgc ccgcggtcga ataccgcatc cgcccggcga accccggcgc ccacctcttc
     4861 gaggtgagct gcacggtggc cgagcccgac cccgcggggc aggtcttcag cctgccggcg
     4921 tggatcccgg gcagctacat gatccgcgag ttcgcgcgca acatcgtgcg cctgcgtgcc
     4981 gaggccgacg gcgagccctg cgcgctggag aagctcgaca agcacacctg gcgcgcggct
     5041 gcggtgccgg gcgcgcgcgt gctgagcgtg cattacgagg tctatgcctg ggacctgtcg
     5101 gtgcgcaccg cccatctcga caccacacac ggcttcttca acggcacgag cgtcttcctc
     5161 gcggtggcgg ggcgtaccga agcaccttgc gtggtgacca tcgaacgccc cgagggcgac
     5221 tgcgggcgcg actggaagct cgtcaccgcg ctgccgcccg agcacggcca cccgggccag
     5281 gcctgccgct tcggccgctt ccgcgccgcg gactacgacg agctcatcga ccacccggtc
     5341 gagatgggcc gtttcacgct ggcacgcttc gaggccgcgg gcgtgccgca cgacatcgcg
     5401 ctcaccggcc gccacgactg cgacctcgag cgcctgtgcg ccgacctgcg ccgcatatgc
     5461 gaatggcaga tcgcgctctt cggcacgccg gcgccggtgg actattacgc cttcctgacc
     5521 atggtggtgg gagagggcta cggcgggctg gagcatcgcg cctcgaccgc gctgatctgc
     5581 agccgcgccg agctgccgtg gaagggcatg gagggtctgc ccgacggcta caagagcttc
     5641 ctcggcctgt gcagccacga gtatttccac acctggaacg tcaagcgcat caagccggtg
     5701 gcgttcacgc cctacgatct cgcgcgcgag aaccacaccc ggctgctgtg ggccttcgag
     5761 ggcttcacct cgtattacga cgacctcgcc ctggtgcgca gcggcgtgat cggcatcgat
     5821 gactacctgg ggctgctcgg caagaccatc gccaacgtct tgcgcggcag cggccggctc
     5881 aagcagagcg tggcggagtc ctccttcgac gcgtggacca agtactaccg ccaggacgag
     5941 aacgcaccca atgccatcgt cagctactac gccaagggtg cgctgatcgc gctcgcgctc
     6001 gacctgcagc tgcgcgcggg cagcgagggt gcggccagcc tggacgacgt gatgcggctg
     6061 ctgtggcggc gccacggcct caccggcgtg ggcgtgccgg aggatggcat cttcgccgcg
     6121 gtgcgcgacg cgggcggcga acgcctcggc gcgcgcctgg cgaaatggct gcagaaggcg
     6181 gtggacggct gcgaggatct gccgttggcg cgcctgctgc gtcccttcgg cgtgagcctg
     6241 cgcgccgagg cggcggggac cgcgccggtg ctcgggatga agctcggcgg gggcagtggc
     6301 gaggcgaagg tcgccaatgt gtacgacgac ggtccggcgc aggcggcggg cgtctcggcc
     6361 ggggacgtgc tgatcgcgct cgacgggctg aggatctcca gcgccaaggg gctggaggat
     6421 ctgctcgccc gtcgtggtgc gggcgacgag gtggaactgc atctcttccg tcgcgacgag
     6481 ctgatgagct tccgcgcggt gctcgctgca ccgcctgccg agcgccagga gctcaagctg
     6541 gcgccgcgcg ccgatagcgc ggcagcgaag ctgcggcggg gttggttggg ggggtgatgc
     6601 gccgccttgc cgtgcgcgag gcgcagccgc ggacgacggc aaggtgtgac ggagcgggca
     6661 cgcacggcca ggcgcgctcg cggcggcagg tcgcgttcag gcctcggggg cggcgtcgtg
     6721 cgccgggaac agcttcggct gccagtagcg ccaggcgagg aagccggcga gccccgcgat
     6781 gatcagccag aagccgacgc cctgccagaa ggcgatcgga tcggtggcga ggaggtagtc
     6841 gcgcggcggg cgcacgaagt ggatccgccc tgtggtcagc gcgcgcaccg cctgtgcgat
     6901 cgccgccagc gcgggcatga accaaaccag cagcccggcg ccgacgatga cgcggtcgag
     6961 cagcggcgtg gcgaggctgc gcgcgctgta gacgctgcgc accccgaacc acaccgcgat
     7021 caggaagccg agcgccgcag cgagcgggcc gatcgccatc acgccgccga gcagggtggc
     7081 cgccgcgagg aagccgagcc cctccaggcg cacgatctcc ggccacaggc gctcgagctg
     7141 gctgaacaac agccacgatg caggcagcgc gaccacgcat accagcagcg cgaacaacgc
     7201 gcggcgccgg tcgaggtcgc ccggcagggg gcgggcgcgg gtttcagcgg aaacggtgac
     7261 ggaagaggaa ggctcgggca tgggaatgga ggcggtggaa aaggttcgga gcccgggaag
     7321 tgtactacgc gcacagcccc gcgcccctgc agttcccgtc acgcgctcgc gcgtcgtcgt
     7381 gatcgcgact tgcgtcgctc ccacgaaaat cggcacgtta ccgcagcacg atgcagcttc
     7441 ggaccccgct gggagcgacg cgagtggcga tcctccgtcc cggctcgcgc cgccctcgtc
     7501 gcgggctccg gcaccgtgat cgggaccggc gtcttcagcc gaaataccca tgcacgaacc
     7561 actgccccgg cgcatccagg ctctcgaaga tccagcacag gacgttgtcg gcatcgcgcg
     7621 cgaggtagta gtcgcgacgg accggcatgc cgtcccacca gccggtctcg atgcgttcgg
     7681 cggcgccgag cagggtatag cggggagcgg agatcggacg cggcgcgggc agcagccaga
     7741 gcgggcgcag gcctgctgtc gtgcgtggcg gcggcgatgc cgtccgtgtc gaggccggcc
     7801 ccgcagcggc gtcgcggccg gcgcgtgcag tggtcgcctg tgcggagggc acggtgtttc
     7861 gccaggcggc ttcgggacgg tgatcggcgc gaagatccag gcgctgcacg cattcctcgc
     7921 caagtcgcgc ctgcaggcgg gcaagcagca gggcggcgtc ctctccggcc tgggcaggat
     7981 cgccgaacag gtcccgggcg cgcgggtcgg cagccacggg gctttccgca cacagggtga
     8041 tcgagtcgac ctcggcggac agctcgagca tggccaggcg ttcgcgcacg atcagggcga
     8101 agtgcccctc gtcgcggctt gggcggccga gcgcgaactc gagttcggtg ggcggtcgcg
     8161 agccgtggcc gaaacgcagg cggaagcggt cgactgccgc gtggcgggca tcgagccagg
     8221 cggcaaggct ggcgaagagg cggcgtgcgg cgaagagcag ggcctcggcg tgaagttccg
     8281 gcgcgggcag ggcgatgccg tggtggaagc gcagcggcgc ttcgaaccag gggcgtggat
     8341 cggcgagctc gccgcgcgcc cgcgccagcg cccgcgcggc cgcctgcgca ccacggcggg
     8401 cgagtccggc gggcggcagg cggcgaagct cgccaagggt gcgcaggccg agtccggcga
     8461 gcaactccag cgtggcggcg tcgcaggcgt cttcctcgct caggagttcg agcggcagcg
     8521 ggtcgagcgc agcgcgccag tcttgcccgg cggcgtcggt ggggagtgcc gcgtcggtcg
     8581 gggggcagga gagggcggaa ggagcggccg cccgtgcaag ccagcgcgcg gccagtggag
     8641 tgggtgcgca gcccagcagg gccgtgaagc ccagctcggc aaggccttcg cgcaaggcgg
     8701 attcgatctg tgccgtgcca ccgaacaggc gcaggcagga ggagatctcc agcagcaccg
     8761 cgtccgctgc ggacaggctg atccgcgggc tgaagcgccc ggcccaggtg gcgatctcgg
     8821 cgagggcttc ggcttcgcgc gccggctcgt gcggttgcag gcgcagttcg ggcagcaagg
     8881 cgagggcgct cgccgcacgc tgccccggtt cgatgccggc cgcgctcgcc gccgcgctgg
     8941 cggccagcac ccggccccgg ggcgacagga tcgccagcgg tggggcggcg cgtacgccgc
     9001 gtgtgaagac ctgcagcgac aacgccggca acaggatcgc cagccacaac atggaaacgc
     9061 tccgcgtcga ggtgtttgaa tcggtcggca gcgagggctg cggttagagc gacgaaacga
     9121 ttgcggcggg tggaggaagg ccgccgatcg cgactggcgt cgctcctaca gaaaacgccg
     9181 cgcttccggt ttgcatgcag gcgcgacggg ggcgacggtg gtggatgcgg tagggtggga
     9241 agctgccgag aggggcatcc ggggggattg ccctgcgcgg gatgccttcg gtaccgggct
     9301 ggccccgggc ggcagggcga gcacgagcgg cgcggcggcg agcggtccgc ggcgcttgag
     9361 gatgcgtagc tgcaggcctt cgggctgcgg ggcgagcgca atccgcaaca ccgccggcga
     9421 aggctgaccg gcgacgctgc cgggacggaa gaggaagagc ggggtggcgc tttcctcggc
     9481 ggccagctgc aggcggcgca gtccggcgtt gtcgatgcgc tgcggccacg ccagcacgca
     9541 ggcgcagctg cccgacagca gtgcctggcg tgcggcccac agcaggtctt cgcggcgctc
     9601 ggggcgcacc acgagcaggc gcgccagcgg cacgcctgcg ttcgccagcg cgggagcgta
     9661 gggcagcagc ggcggggcga tgcagacgat ccagcatgcg ggcggggtgg cgccgagcag
     9721 cggcagcagc aggctcagtt cgccgacgcc ggggcggtcg gcgaggagct cgatgagcgc
     9781 cccggccggc cagccgccgc cgggcagctc ggcgtcgagc gcggagaagc cggtcggcag
     9841 caccgctcct gcggcgcctg cgagccggct cgcctgccac acgctgcccg caggcaggcc
     9901 ggcgagcacg ccatgcgtgc cggtggcggc aggagctaca gcgcgcagcg acgcgtcggc
     9961 gtggcgttgg agcgggaggg ccatggcggg tgcggcatcg gctcgctcag tggtccttgc
    10021 ggatcagccc caccatgacg ccttcgatga ccagcggctc gcggcgggtg tccacgacga
    10081 tcggggtgta gtccgggttg gcggggagga gctcgaccac cgggcccttg cgttgcaggg
    10141 tcttgacggt gacgtcgtcg tccacccggg cgaccacgat ctggccgttg cgcgcctcgc
    10201 tgttgcgatg caccgcgatg aggtcgccgt cgaggatgcc ggcgtcgcgc atgctcatgc
    10261 cgcgcacgcg cagcaggtag tcggccctgg gcgagaacag cgcggggtcg agctgatagc
    10321 gcgcctcgac gtgttcggcg gcgaggatgg ggctgcccgc ggcgacgcgg ccgaccaggg
    10381 gcaggccgag cgcctcggcc aggcggatgc cgcgcgcgcg cccctcctcg agcaggatcg
    10441 cgcccttggc ggcgagcgcg cgcaggtggg tctcggcggc gttgggcgag cggaagccga
    10501 aggcggtgca cacctcggcg cgggtgggcg ggcggccttc ggactcgacg gtggagcgga
    10561 tgaaatcgag gatttcctgc tggcgcgcgg tgaggttttc gctacgggcg gtcatgacag
    10621 cctccatggg atggctgtaa ttttaaacag tcccgcagcg ctggcaagcg gaattcaggg
    10681 cagcagcacc gccgccccct gcagcgcgcc gcggcgcagg cgctcgagcg cctcgttggc
    10741 gtcctcgagg gcgaagcgga cgacctcggt gcgtaccggc accctgggcg cgagggcgag
    10801 gaattcgtcg ccgtcgcggc gggtgaggtt ggcgaccgag acgatgcggc gctcgcccca
    10861 caggatgtcg taggggaagg cggggatggt gctcatgtgg atgcccgcgc acaccaccgt
    10921 gccgcccttg gcgacggcgc gcagcgcggc ggggacgagc tcgccggcgg gggcgaagat
    10981 cagcgcggcg tcgagcgggt gcggggggcg ctcttcgccc gcgcccgtcc agcaggcgcc
    11041 gagccggcgg gcgaaggcct ggcttgcgct gtcgccggga cgggtgaagg cgtacacctc
    11101 gcgaccctgc cacaccgcca cctgggtgat gatgtgggcg gccgcgccga agccgtagag
    11161 gccgatgcgg cgcgcggcgc ccgccatctc cagcgcacga tagccgatca ggccggcgca
    11221 cagcagcggc gcggcctcgg cggcgtcgag cgcctcgtcg atcgggaagc agtagcgtgc
    11281 gtcggccacg gtgtattcgg cataaccgcc ctggatctgg tagccggtga agcgcgccgc
    11341 gtcgcagagg ttctcgcgct ggctggcgca gaagccgcaa tgcccgcagg tccaccccag
    11401 ccagggcacg ccgatgcgct gacccggcac catgcccgcg gcgtcgccga cgccttcgcc
    11461 cagttcgacc acgcggccga tgatctcgtg gccgggaatc atcggcagca ccgggtcggg
    11521 cagctcgccg tcgatgaggt ggagatcggt gcggcacacg ccgcaggcct ccacccgcag
    11581 cagcacctgc ccggggccgg ggcgcggcac cgccagatgc accgcgcgca gggcctcgcc
    11641 ggggcgttcg agttgcatcg cgcgcatgcc ggcggggggc ttggcggggg cgttcggatg
    11701 gggccggggc atgcttgcgg tctccaggac aggcggaacg atggtgcgct gcgacttgag
    11761 tacggccccg atctgggcta ttcgttggac acggagccgc atcgcggcac ccgacacgac
    11821 gacaaggaaa agggaggaga cccccatgct cgaccagtcc gaagcctgcg gtcccggcgg
    11881 tagtgttcgc ctggggtctc ggctagaatc cggcccacat cagggagaac acatgctgca
    11941 gagcggagac atcgcccccg cgttttcgtt gccggatgcc gacatggaaa ttttcgacct
    12001 cgcgtcggtg gccggcaagc acaatgtggt tctctacttt taccctcgcg acaagacccc
    12061 gggctgcacg cgtcaggccg ccgatttcag cgaccacgac gacgagttcg cccgctacga
    12121 ctgcatcgtg gtcggcgtga gcccggacga ttgcctgacc cacgccgagt ttcgcgacca
    12181 ggaaggattg tcgatccgcc tgctctccga ttcggacacc gaggtgtgcc ggctctacaa
    12241 cgtatggcat gagcgcgaag tcgacgggat gaagaagatg ggcgtgcggc ggtcgacctt
    12301 cgtgatcgac aagcagggca ggatccgcca cgcgctctac gatgtgacac cccgcggcca
    12361 cgccgccgcg gttttcgaat tggtcaagga actggaaccc cagcatgcaa aacgaaatcg
    12421 tcaagaatag cgtggtgacg ctgaagtaca ccgtgcgcga ccccgaaggc gcggtgatcg
    12481 acgacggcga gcacccgctc gtttatctgc acggcggcta cgatggcatc tttccgttgc
    12541 tcgaagaggc cctgcacggc aagaaggtcg gcgagcagct gcaggtcaag ctgcagcccg
    12601 aagacgcctt cggcgactac gacgaggagc tgatcctggt cgaggacgcc aagcttttcc
    12661 cggacaacat cgagatcggc atgagcttcg agcgcgtctc cgaggacggc gaggaagaca
    12721 tgatctaccg catcaccgac atcgccgacg gcaaggtcgt ggtcgatggc aaccacccgc
    12781 tggccggcgt ggcgctggtg ttcgacgtca ccgtcgccga ggtgcgcgcg gcgagcgccg
    12841 aggagatcac ccacggccat gtgcatggtc ccggtggtca tcaccaccac tgattgcacc
    12901 gatgtcagga cggggccgcg caagcggccc tttcgacatg tggacggcac ctgcggctgc
    12961 gtggtgcgtg tcaggggccg tccgcctcgt ccgcgcctcg ttcgcctctc tccgccttcc
    13021 tccgcctcga tctgctgcgg ccctgcaagg gtcgtcccac gcgcatgaac gctccgctcc
    13081 ccgccgatct ccctgaagcc gcgaccgctg cgctgctgca cgtcgaagga ctgggcgtgg
    13141 cgatcgcgcg caccgacggc gaggaggtcc gcccggtcga cggggtgaat ttcgccatcg
    13201 ccgcgggcga gaccttcgcc ttgctcggcg aatcgggctg cggcaagtcg atgaccgcgc
    13261 tcgcgctcgc ccgcctgctg cccgacggcg gccgcatcgc cgcgggcagc gtgcgcctgg
    13321 gcgacgagga cctgctcgcg ctgcccgagg cggcgatgcg gcgggtgcgt ggcgggcgca
    13381 tcggcatgat cttccaggag cccgccacca gcctcaaccc ggtgatgagc gtgggtgcgc
    13441 agatcgccga ggcgctcgcc gcccacggct tgcgcggcgc ggcggcgcgc gcagaggcct
    13501 gccgtctgct cgccgcggtg ggcatccccg accccgagcg ccggctgcag gagtttccct
//
LOCUS       DAOQBS010000081        13504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3628_length_13504_cov_14.806751, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000081 DAOQBS010000000
VERSION     DAOQBS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 13504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13504
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3628_length_13504_cov_14.806751"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PKN25_10075"
     CDS             <1..869
                     /locus_tag="PKN25_10075"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNV91290.1"
                     /translation="VPVGAPRGAIAAPRWVLKTLLPAHEHDEDARRALVREEWLARRV
                     PAQGFPQVTDWPARAHLYYLMSWHEGESLKALLARGERLRAHEVAELGARVLRLVGVL
                     HRLGIVHRDIKPDNLHLGRDGVLRLLDLGVAASEAEDLREINNPGTPSYMAPELFAGT
                     PANEASDLYACGVTLYELLTRKFPYGEVEPFQHPRFGEPVPPTRHRPDTPAWLEAVLL
                     KACAREPAARFETAEEFRLALERGAWQPLAVPRRTPLLERRPTLALKLIALASLLLNV
                     VLLFLLYRSLKS"
     gene            942..3743
                     /locus_tag="PKN25_10080"
     CDS             942..3743
                     /locus_tag="PKN25_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV91291.1"
                     /translation="MTHPLPEPARPSPAGRRTIPIRAAAPGETPATCPYCGVGCGVLI
                     EHDGTRITGVRGDPAHPANFGRLCTKGSTLHLTAGHDTRLLYPEYRARRGEARTRIGW
                     DAAIDVAAQRFADVIAEHGPDAVAFYISGQLLTEDYYVFNKAMKGLIGSNNVDTNSRL
                     CMSSAVAAYKATLGADAPPCCYEDFDHADTILIAGANPAWAHPVAFRRIEEARARRPG
                     MKIVVVDPRRTDTAAMADLHLAILPGTDIWLFDAMLHVLLRDGLADEAWIAAHTEGFE
                     ALRAHVHGVSPAVAAAVCGVAAEDIVTAARWWGEAPAALSLWCQGLNQSTHGTHNGAA
                     LIALSLATGKIGRPGCGPFSLTGQPNAMGGREVGGLANLLPAHRDLANPAHRAEVARL
                     WGVDSVPSEPGLTAVELFRAAREGRVKAIWIACTNPAQSMPEQALVHEALAACDFVVL
                     QEAFAGTETAAFADLMLPAATWGEKEGTVTNSERRISRVRAAVPAPGEARADWRIVCD
                     FARALAPRIGKPQAAAMFAYASAQAIFAEHVASTAGRDLDITGLDYSLLEGEGPQQWP
                     FPAGAAVDGADAAARARLYADGRFATPNGRARFVVPARALTAEKTGVRYPLALTSGRL
                     RDQWHGMSRSGKVARLYGHADEARIELHPQELARRGLVDGDLVKVSSRHGELVLRVAA
                     ASALRHGMAFVGMHWGRRALNSAGINLLFGGACDPLSKQPELKHAAVRIEPVELPNRM
                     LLVRAERPGRTAAEEAALLSPWLERFAYASLALAGRDTPAVVMRVAHDRPIPAGWLAE
                     LDALLGLEGDGVLSYADPARGISKRALIEDGRLVGLRLTGETAAAGWLSEAVVERRDA
                     AALRPWLLAPLATPPTGDAGRGRVVCNCLNVSEADIAAAIAAGAGVRDFDALQARLRC
                     GTSCGSCVPEIRRMLAEGS"
     assembly_gap    3938..4037
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4125..5093)
                     /locus_tag="PKN25_10085"
     CDS             complement(4125..5093)
                     /locus_tag="PKN25_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNV91292.1"
                     /translation="MEKIVFLDRATLAPQIRLRRPSFAHDYAEHARTAPAEVVERLAG
                     ATIAILNKVRLDAAAIAQLPALRLVAVAATGTDCVDKAACAARGIAVCNIRGYARDTV
                     PEHVFALLLALRRQLIPYRDDVLAGEWQRAGQFCFFNHPIHDLAGTRLGIVGAGDLGQ
                     RVAAIARAFGMEPVFAARKGERPGDDSYLAWDEVLATSDVISLHCPLTEATRGLVGLP
                     EFRAMARRPLLINTARGGIVDEHALVQALDEGLISGAGFDVTDGEPPAPDSPLMRIAA
                     RPNVLLTPHIAWASDEAQQALADQLVDVIEKFVAGQPKNLVSGGFA"
     assembly_gap    5348..5447
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5615..7405)
                     /locus_tag="PKN25_10090"
     CDS             complement(5615..7405)
                     /locus_tag="PKN25_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV91293.1"
                     /translation="MNPPSKRATPPTHAGRFAEPFAPPPPRNPLDRRVHAAIARATAS
                     VSPIALLLATVDWAGHLAVSPGKRMELADLGIAQMRRLLRYAQQLAIAPAGSAAHECI
                     EPPAQDRRFQAPEWHAWPFNLMHQSFLLGQEWWDAATHGVAGVSRHHEQVVAFAARQL
                     LDMFSPGNFLPTNPVVLRQTLASGGMNLARGAVHAVEDFERLVGGAPPAGAEDFVVGR
                     DVAVTPGKVVLRNRLIELIQYAPATADVYAEPVLIVPAWIMKYYILDLSPHNSLVRYL
                     VERGHTVFCISWKNPGTDERELDMDDYLQLGFFAALDAINAIVPGQKVHATGYCLGGT
                     LLAIAAAAMDRDGDARLGSMTLFTAQTDFTEPGELALFIDDSEVSLLEAQMEETGFLT
                     AGQMAGAFQILRSNDLLWSRVVGEYLMGERTPMNDLMAWNADATRMPARMHSQYLRRL
                     FLNDDLSEGRYPVGGKPVALSDIELPVFCVATLTDHVAPWRSVYKLHYLVPTEISFVL
                     TSGGHNAGIVSEPGRPRRSFRMHTRPAGGNYVPPDDWLERTPEQEGSWWPAWSDWLAA
                     RSGARVAPPAIGAPDYPALDDAPGCYVHEK"
     gene            complement(7432..10926)
                     /locus_tag="PKN25_10095"
     CDS             complement(7432..10926)
                     /locus_tag="PKN25_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV91294.1"
                     /translation="MINVVSRDRLRNLADFLLPVLLMAAILSATSLLGHVRLKDATAD
                     IERETHLRLEQIAEVDEIGDLLGRIHAELHRIVMPGVAPGPDPAAQVLAALWLDNQLA
                     ALETRVDAIGVWSTAQASHARAKASLQSYRTLVLPLLATDAREPRAAALDLAHADRLH
                     NALVRHTEELAIAVTADAAEKNADAIHIIQRRLDQSLSTSLTVIVLTLLLAFTLAKRL
                     ANDRNTLADALRRMAQDQLEAEHYEALTRLAAKKGDRLGELAQALLGFRETRLAQKDD
                     ETLMKAVIEQAPCAIELVDPVTLRFLRANAYSRQALGYTEAELRQRSVMDIQAELQPA
                     QLEALARDIVARRSANFETVHRASDGRLLEARVHIRVIRLHGQDYLLGIWQDVSAEHQ
                     ALQALKKFSMAIEQSPNAVLITDTEARIEYVNDAFLHTSGYTRAEVIGRNPRLLKSDK
                     TPAEVYAQLWDALVKGEPWRGELVNRRKDGGEYIEQAQISPIRQPDGRITHYLAIKQD
                     ITEKRRMLEELEHHRHHLEQLVAERSAALHTALDAAEVANRAKSEFLANMSHEIRTPL
                     NAIIGLGHLLARDLREDEQKARLEKIITAARHLLQIINDILDLSKIEAGRLDLEANDF
                     DPGAMLEGTLELVREQAEQKGLQLSLDLDMLPSRVRGDALRLGQIVLNFASNAVKFTP
                     HGSVAIRGRVLSTAGEQSIVRFEVADTGIGVDASQCERMFQPFEQADPSTTRKYGGTG
                     LGLAISRRLTELMGGRIGVDSTLGKGSTFWVEVPLQLRPASAGRQPSAAAGAPPSAEH
                     DRLCGRRILLAEDNPVNREVAVAILRGTGAVIDTAENGQEAVDMASTTRYDLILMDMQ
                     MPVMDGITATRLLRSRDELRKLPILAMTANAFAEDRQRCIDAGMNDHITKPIEPQALC
                     EKIWRWLPPDAPAADLPTPPAVQAGEEPVDDDPLAPLAGLHELDVATGLQYVLDDREI
                     YLGLLRLFAESDYGKLLQESIDAGDAVALRRAAHSIKGGAATIGATALSLAAAELEAR
                     VVSGLADEAQSMQAGPLHEAASALAARTTRFCADLEAATREAAADAASAPERSDEDCV
                     EAAPARTAEIVATVEHQLTLGDIAVRELLEAHRGLLRNVLGERFAHFEERIGNFDFET
                     ALDDLRAADAARKDTQAR"
     gene            complement(10946..12076)
                     /locus_tag="PKN25_10100"
     CDS             complement(10946..12076)
                     /locus_tag="PKN25_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HNV91295.1"
                     /translation="MPADSSTRSTVLIVDDTPENLTILGETLRPHYRVRAAISGERAL
                     HAVTIGTLPDVILLDVMMPDMDGYQVLQALKADAATRDIPVIFVTAMDSVEGETHGLA
                     LGAVDYITKPFNPDIVLARVRTHIELAKARERLHDENAWLEREVARRTNENALIRDLS
                     LSALACLAEVRDIETGHHILRTQSFVELLAHHLAGHPDYRAALAGERLEIIVRSAPLH
                     DIGKVGIPDSILLKPGRLTSAEFEIMKKHPVIGADAITRAMAQAANSRSGDGALPGGA
                     FAFMETAREIALSHHEKWDGSGYPAGLQGRQIPVSARLMALADVFDALSSRRVYKEPL
                     APEDVTRIIVAERGRHFDPVIVDAFLELRPAFAEVAQRFAEA"
     gene            complement(12285..13460)
                     /locus_tag="PKN25_10105"
     CDS             complement(12285..13460)
                     /locus_tag="PKN25_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV91296.1"
                     /translation="MVRHPTPPKQPGQAGRSTHLRNTWNLGLKEFRSLSRDLAMVFLI
                     VFMFSASIYADARAKPESLSRAAIAVVDEDGSQLSGRIVAALQPPQFLPPEHVGLADM
                     DRGLDAGRHTFVLDIPPNFQRDVLAGRSPALQLNIDATRQGQAQTGAAYIQNILADEV
                     RAFVQRMRAPAPQPVELVPRAMFNPNLEASWFAAINAIINNVTMLGILLTGAALIRER
                     EHGTIEHLLVMPVTPLEIMLSKIWSMGVVVLVASSFSLQVMVKGVLGIDVAGSALLFG
                     AGTLLYLFAAASIGIYMGTIARSMPQFGLMSILILLPLQILSGGVTPRESMPEAIQTI
                     MGLAPTTHYVSLAQSILFRGAGLDVVWPAFAMVAAIGAVFLALAHHRLRHTIGTMQG"
BASE COUNT         1838 a         4376 c         5009 g         2081 t
ORIGIN      
        1 cggttcctgt gggagcgccg cgaggcgcga ttgcggcgcc gcgctgggtc ctcaagaccc
       61 tgctgcccgc ccacgagcac gacgaggacg cgcgccgcgc gctggtgcgc gaggagtggc
      121 tggcgcggcg ggtgccggcg cagggctttc cgcaggtgac cgactggccc gcgcgcgcgc
      181 atctgtatta cctgatgagc tggcatgagg gcgagagcct gaaggcgctg ctcgcgcgcg
      241 gcgagcgcct gcgcgcgcac gaggtcgccg agctcggcgc gcgcgtgctg cgtctggtcg
      301 gcgtgctgca ccggctgggc atcgtgcatc gcgacatcaa gcccgacaac ctgcacctgg
      361 gccgcgacgg cgtgctgcgc ctgctcgacc tcggcgtggc ggcgagcgag gcggaggacc
      421 tgcgcgagat caacaacccg ggcacgccgt cctacatggc gcccgagctc ttcgccggca
      481 cgccggccaa cgaggcctcc gatctctacg cctgcggggt gacgctgtac gagctgctca
      541 cgcgcaagtt cccctacggc gaggtcgagc ccttccagca cccgcgcttc ggcgagcccg
      601 tcccgcccac ccgccaccgc cccgacacgc cggcctggct ggaggcggtg ctgctcaagg
      661 cgtgcgcgcg cgaacccgcc gcccgcttcg agaccgccga ggagttccgc ctcgcgctcg
      721 agcgcggcgc ctggcagccg ctcgcggtgc cgcgccgcac gccgctgctc gagcgccggc
      781 cgacgctggc gctgaagctg atcgcgctgg cctcgctgct gctcaatgtg gtgctgttgt
      841 tcctgctgta ccgatccctg aagtcctgaa gggggtcagg cctttcattt gctcatcctg
      901 tgaagacccg atccctcaga cccctacgga cgaggcaaac catgacccat cccctgcccg
      961 aacctgcccg gccttcgccc gctggccggc gcacgatccc gatccgcgcc gcggcgccgg
     1021 gcgagacccc tgccacctgt ccctactgcg gcgtcggctg cggcgtgctg atcgagcacg
     1081 acggcacccg catcaccggc gtgcgcggcg accctgcaca tccggccaac ttcggccgcc
     1141 tgtgcaccaa gggctcgacc ctgcacctca ccgccggcca cgatacccgc ctgctctacc
     1201 ccgaataccg cgcccgccgc ggcgaggccc gcacgcgcat cggctgggac gctgcgatcg
     1261 acgtggccgc gcagcgcttc gccgacgtga tcgccgagca tgggccggac gcggtcgcct
     1321 tctacatctc cggccagctc ctcaccgagg actactacgt cttcaacaag gcgatgaagg
     1381 ggctgatcgg cagcaacaac gtcgacacca actcgcgcct gtgcatgtcg agcgcggtcg
     1441 ccgcctacaa ggccacgctg ggtgcggacg cgccgccgtg ctgctacgag gacttcgacc
     1501 acgccgacac gatcctgatc gccggcgcca acccggcgtg ggcgcatccg gtggccttcc
     1561 gccgcatcga ggaggcgagg gcgcggcggc cgggcatgaa gatcgtcgtg gtggatccgc
     1621 gccgcaccga caccgcggcg atggccgacc tgcacctggc catcctgccc ggcaccgaca
     1681 tctggctgtt cgacgccatg ctgcacgtgc tgctgcgcga cgggttggcc gacgaagcct
     1741 ggatcgccgc ccacaccgag ggtttcgagg cgctgcgcgc gcatgtgcac ggggtgagcc
     1801 cggcggtggc cgcggcggtg tgcggggtgg cggcggagga catcgtcacc gcggcgcgct
     1861 ggtggggcga ggcgccggcg gcgctgtcgc tgtggtgcca ggggctcaac cagtcgacgc
     1921 acggcaccca caacggcgcc gcgctgatcg cgctctcgct cgccaccggc aagatcggcc
     1981 gccccggctg cgggccgttc tcgctcaccg gccagcccaa cgcgatgggc gggcgcgagg
     2041 tcggcgggct ggcgaacctg ctgccggcgc atcgtgacct ggcgaatccc gcgcatcgcg
     2101 cggaggtggc gcggctgtgg ggcgtggaca gcgtgccttc ggagcccggc ctgaccgcgg
     2161 tggagctgtt ccgcgcggcg cgcgagggcc gcgtcaaggc gatctggatc gcgtgcacca
     2221 acccggcgca gtccatgccc gagcaggccc tggtgcacga ggcgctggcg gcctgcgact
     2281 tcgtcgtgct gcaggaggcc ttcgccggca ccgagaccgc ggccttcgcc gacctgatgt
     2341 tgccggcggc cacctggggc gagaaggagg gcacggtgac caactccgag cgccgcatca
     2401 gccgcgtgcg cgcggccgtg ccggcgccgg gcgaggcgcg tgccgactgg cgcatcgtgt
     2461 gcgacttcgc acgcgcgctc gcaccgcgca tcggcaagcc gcaggcggcg gcgatgttcg
     2521 cctacgcgtc ggcgcaggcg atcttcgccg agcacgtcgc cagcaccgcc gggcgcgacc
     2581 tcgacatcac cgggctggac tattccctgc tggaaggtga aggcccgcag cagtggccgt
     2641 ttccggcggg agcggcggtg gatggcgccg atgccgcggc gcgggcgcgt ctctacgccg
     2701 acggtcgctt cgcgacgccg aacgggcgcg cacgcttcgt cgtgccggcg cgcgcgctca
     2761 ctgccgagaa gaccggcgtg cgctatccgc tcgccctcac cagcggccgc ctgcgcgacc
     2821 agtggcacgg catgagccgc agcggcaagg tggcgcgcct gtacggccat gcggacgagg
     2881 cacgcatcga gctgcacccg caggagctgg cgcggcgcgg cctcgtcgac ggcgacctgg
     2941 tcaaggtgtc gagccggcac ggcgagctgg tgctgcgcgt ggccgcggcg agcgcgctgc
     3001 ggcacggcat ggccttcgtc gggatgcact gggggcggcg ggcgctgaac tcggccggga
     3061 tcaacctgct cttcggcggc gcctgcgatc cgctgtcgaa gcagccggag ctcaagcacg
     3121 cggcggtgcg catcgagccg gtcgagctgc cgaaccgcat gctgctggtg cgtgccgagc
     3181 gccccgggcg taccgccgcc gaggaggctg cgctgctgtc gccctggctg gagcgcttcg
     3241 cctacgcctc gctcgcgctc gccggccgcg acacgcccgc ggtggtgatg cgggtcgcgc
     3301 acgaccgtcc gatcccggcc ggctggctcg ccgagctcga cgccttgctc ggcctcgagg
     3361 gcgacggggt gctgtcgtat gccgaccccg cgcgcgggat cagcaagcgt gcgctgatcg
     3421 aggacggccg cctggtcggc ctgcgcctga ccggcgagac cgcggccgcg ggctggctga
     3481 gcgaggcggt ggtcgagcgc cgcgacgcgg cggcgctgcg tccgtggctg ctggcgccgc
     3541 tggcaacccc gccgacagga gatgcggggc gcgggcgggt ggtgtgcaac tgcctgaacg
     3601 tgtccgaggc cgacatcgcg gcggcgatcg ccgcgggcgc cggcgtgcgc gacttcgacg
     3661 cgctgcaggc caggctgcgc tgcggcacgt cctgcggttc gtgcgtgccc gagatccgcc
     3721 gcatgctcgc cgaggggagc tgaaaagaaa aagcccgggc gtcttgcgag gcccgggcaa
     3781 atccacacca aaggaggagg gtggaggaga caggtgcaat cgtagagaaa ttttttgtgc
     3841 agtgcaacaa aaaataatgc ttcgactata aggcgaattt ctagattttg gccgaatgca
     3901 tcgcgacttg cgtcgctcct acgtcccaca ccgggcgnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnttc tgtgggagcg acgcaagtcg cgatcgcagc gcgcgggatc
     4081 cgcgccgcga ttgatcgatc gctggatcgc gactcgcgtc gcgcctacgc aaacccgccg
     4141 gagacgagat tcttcggctg gccagcaaca aatttttcga tgacatcgac cagctgatcg
     4201 gccagcgcct gctgcgcttc atccgaggcc caggcgatgt gcggggtgag gaggacgttc
     4261 gggcgggcgg cgatgcgcat cagcgggctg tcgggcgcgg gcggttcgcc gtcggtgacg
     4321 tcgaagccgg cgccggagat caggccctcg tcgagcgcct gcacgagggc gtgctcatcc
     4381 acgatgccgc cgcgcgcggt gttgatcagc aggggacgcc gcgccatcgc gcggaattcg
     4441 ggcaggccga cgaggccgcg ggtggcctcg gtgagcggac agtgcaggct gatgacgtcg
     4501 ctggtcgcga gcacttcgtc ccaggccagg tagctgtcgt cgccggggcg ttcgcccttg
     4561 cgcgcggcga acaccggctc catgccgaag gcgcgcgcga tcgccgccac gcgctggccg
     4621 aggtcgccgg cgccgacgat gccgagccgg gtaccggcga ggtcgtggat ggggtggttg
     4681 aagaagcaga actgcccggc gcgctgccat tcgccggcga gcacgtcgtc gcggtagggg
     4741 atgagctggc ggcgcagcgc gagcaggagc gcgaagacgt gctcgggcac ggtgtcgcgc
     4801 gcgtagccgc ggatgttgca caccgcgatc ccgcgcgccg cgcaggcggc cttgtcgacg
     4861 cagtcggtgc cggtggcggc gaccgcgacc aggcgcagcg cgggcagttg cgcgatcgcc
     4921 gcggcgtcga ggcggacctt gttgaggatc gcgatggtgg cgccggcgag gcgttcgacg
     4981 acctcggcgg gggcggtgcg cgcgtgttcg gcgtagtcgt gggcgaagga cggacggcgc
     5041 aggcggatct gcggcgcgag cgtggcgcgg tcgaggaaga cgatcttttc catggggggc
     5101 tctgtggggg aacgaagcgg atcgccgggg cgggcgatgg ggaggggggg cggagaggtg
     5161 gaatcgcgac ttgcgtcgct cccacaggaa gaccgcggcg ctccgacggc aagaacgcgg
     5221 cgctcgcgca gcaggaccgc ggcgctcggg gtggctgtgg gagcgacgca agtcgcgatc
     5281 aaggcggcag cgctgcgctg ggcgcaggcg gatcgaggcg ggcgggaatc gcgacttgcg
     5341 tcgctccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctg cgctgggcgc
     5461 aggcggatcg aggcgggcgg gaatcgcgac ttgcgtcgct cctacacccg ctcctacacc
     5521 cgctcctgca tcccgccgcg ggcgtagcgg gttgtgcggg gcgcggcggg ggaggtcgca
     5581 tcggcggcgc ggtcaagctg cccgcatcgt ccgctcactt ctcatgcacg tagcagcccg
     5641 gcgcgtcgtc gagcgcggga tagtctgggg caccgatcgc cggcggcgcc acgcgcgcgc
     5701 cgctgcgcgc ggcgagccag tcgctccagg ccggccacca cgagccttcc tgctcgggcg
     5761 tgcgctcgag ccaatcgtcc ggcgggacgt agttgccgcc cgcggggcgg gtgtgcatgc
     5821 ggaagctgcg ccgcgggcgg ccgggctcgc tgacgatgcc ggcgttgtgg ccgccgctgg
     5881 tgagcacgaa gctgatctcg gtcggcacca ggtagtgcag cttgtacacc gagcgccagg
     5941 gcgcgacgtg gtcggtgagg gtggcgacgc agaacaccgg cagctcgatg tcggacagcg
     6001 ccaccggctt gccgcccacc gggtagcgcc cctcgctgag gtcgtcgttg aggaagagcc
     6061 ggcgcaggta ctggctgtgc atgcgcgcgg gcatgcgcgt ggcgtcggcg ttccaggcca
     6121 tgaggtcgtt catcggcgtg cgctcgccca tgaggtattc gccgacgacg cgcgaccaca
     6181 gcaggtcgtt ggagcgcagg atctggaacg cacccgccat ctggccggcg gtgaggaagc
     6241 cggtctcctc catctgcgcc tcgagcaggc tgacctcgct gtcgtcgatg aagagcgcga
     6301 gctcgcccgg ttcggtgaag tcggtctgcg cggtgaacag cgtcatcgag ccgaggcgcg
     6361 catcgccgtc gcggtccatc gccgccgcgg cgatcgccag cagggtgccg ccgaggcagt
     6421 agccggttgc gtgcaccttc tgcccgggga cgatggcatt gatcgcgtcg agcgcggcga
     6481 agaagccgag ctgcaggtag tcgtccatgt cgagctcgcg ctcgtccgtc ccggggttct
     6541 tccacgagat gcagaacacg gtgtggccgc gctcgaccag gtagcgcacc agcgagttgt
     6601 gcggcgagag gtcgaggatg tagtacttca tgatccaggc cggcacgatc agcaccggct
     6661 cggcgtacac gtcggcggtg gcgggcgcgt actggatgag ctcgatgagg cggttgcgca
     6721 gcaccacctt gcccggggtg accgcgacgt cgcggccgac gacgaagtcc tcggcgccgg
     6781 ccggcggggc gccgccgacg aggcgctcga agtcctcgac ggcgtggacg gcgccgcgcg
     6841 cgaggttcat gccgccgctc gccagggtct ggcgcagcac taccgggttg gtgggcagga
     6901 agttgcccgg cgagaacatg tcgagcagct ggcgcgcggc gaaggcgacg acctgctcgt
     6961 gatggcgcga gacgccggcc acgccgtggg tggcggcgtc ccaccattcc tggccgagca
     7021 ggaaggactg gtgcatcagg ttgaagggcc aggcgtgcca ctcgggcgcc tggaagcggc
     7081 ggtcctgcgc gggcggctcg atgcactcgt gcgcggcgct gcccgccggg gcgatggcga
     7141 gctgctgtgc gtagcgcagc aggcggcgca tctgcgcgat gccgaggtcg gcgagctcca
     7201 tgcgcttgcc cggcgagacc gcgaggtggc cggcccagtc caccgtcgcg agcagcagcg
     7261 cgatcggcga caccgaggcg gtggcgcgcg cgatcgccgc gtgcacccgg cgatcgagcg
     7321 ggttgcgcgg cggtggcggg gcgaagggct cggcgaagcg accggcgtga gtgggcgggg
     7381 tcgcgcgttt gctgggcggg ttcatcctgt acttgcctcc atgcatccgg ttcagcgggc
     7441 ttgcgtgtcc ttgcgcgctg cgtcggccgc ccgcaggtcg tcgagggcgg tttcgaaatc
     7501 gaagttgccg atgcgctcct cgaagtgcgc gaagcgctcg ccgagaacgt ttcgcagcag
     7561 cccgcggtgg gcttcgagca gttcgcgcac cgcgatgtcg ccgagggtga gctggtgctc
     7621 gacggtggcg acgatctccg cggtccgtgc cggcgccgct tcgacgcagt cctcgtcgga
     7681 tcgctccgga gcggacgctg cgtcggctgc ggcttcgcgt gtcgccgcct ccaggtctgc
     7741 gcagaaacgg gtggtgcgag cggccagcgc gctcgcggct tcatgcagcg gaccggcctg
     7801 catggattgt gcctcgtccg ccaggccact gaccacccgg gcttccagct cggcggcggc
     7861 aaggctcagt gcggtcgcgc cgatcgtggc cgcaccgccc ttgatcgagt gggcggcgcg
     7921 gcgcagggcg acggcatcac ccgcgtcgat cgattcctgc aggagcttgc cgtagtcgct
     7981 ctccgcgaac aggcgcaaca gcccgaggta gatctcccgg tcgtcgagga cgtactgcag
     8041 acctgtcgcg acatcgagct cgtggagccc cgcgaggggg gcgaggggat cgtcatccac
     8101 gggttcctcg cccgcctgga ctgccggcgg ggtgggcagg tcggcggccg gggcgtcggg
     8161 gggaagccag cgccagatct tctcgcacag ggcctgcggc tcgatcggct tggtgatgtg
     8221 gtcgttcatc cccgcgtcga tgcagcgctg gcgatcctct gcgaaggcgt tggcggtcat
     8281 cgccaggatg ggcagcttgc gcagctcgtc gcggctgcgc aggaggcggg tggcggtgat
     8341 gccgtccatg accggcatct gcatgtccat gaggatgagg tcgtagcggg tggtgcttgc
     8401 catgtcgacc gcctcctgtc cgttctccgc ggtatcgatc accgctccgg tcccacgcag
     8461 gatggcgacg gcgacctcgc ggttgacggg gttgtcttcg gcgaggagga tgcgccgccc
     8521 gcacagccgg tcgtgttcgg cgctgggggg ggcgccggcg gccgcgctcg gttgccggcc
     8581 cgcgctggcg gggcgcaact gcagcggcac ctcgacccag aaggtgctgc ccttgcccag
     8641 cgtgctgtcg acgccgatcc tgccacccat gagttcggtc aggcggcgcg agatcgccag
     8701 gccgaggccg gtgccgccgt acttgcgtgt ggtggacgga tcggcctgct cgaaaggctg
     8761 gaacatgcgc tcgcactggc tcgcatccac gccgatgccg gtgtcggcga cctcgaagcg
     8821 cacgatcgac tgctcgcccg cggtggacag cacgcggccg cggatcgcca cgctcccgtg
     8881 tggcgtgaac ttcaccgcgt tgctggcgaa gttgagcacg atctggccga ggcgcagcgc
     8941 gtcgccgcgc accctggagg gcagcatgtc gaggtcgagc gagagctgca ggcccttctg
     9001 ctcggcttgc tcgcgcacca gttccagcgt gccctcgagc atcgccccgg ggtcgaagtc
     9061 attcgcttcg aggtccagcc gcccggcctc gatcttcgac aggtcgagga tgtcgttgat
     9121 gatctgcagc aggtgccggg ccgcggtgat gatcttctcc agccgggcct tctgttcgtc
     9181 ctcgcgcaga tccctggcga gcaggtggcc gagtccgatg atcgcgttca gcggcgtgcg
     9241 gatctcgtgg ctcatgttgg cgaggaactc gctcttggcg cggttggcga cctcggcggc
     9301 gtcgagtgcg gtgtgaagcg cggcggagcg ttcggcgacg agctgttcga ggtggtggcg
     9361 gtggtgctcc agctcctcga gcatgcggcg cttttcggtg atgtcctgct tgatcgcgag
     9421 gtagtgcgtg atgcgcccgt cgggctggcg gatgggcgag atctgcgcct gctcgatgta
     9481 ttcgccaccg tccttgcgcc ggttgaccag ctcgccgcgc cagggctctc ccttgacgag
     9541 agcgtcccag agttgggcat acacctccgc gggggtcttg tccgacttga gcaggcgcgg
     9601 gttgcgaccg atcacctctg cgcgcgtgta gccgctggtg tgcaggaagg catcattcac
     9661 gtattcgatg cgggcctcgg tgtcggtgat gaggaccgcg ttggggctct gctcgatcgc
     9721 catggagaat ttcttcaagg cctgcaaggc ctggtgttcg gccgacacgt cctgccagat
     9781 gccgagcagg tagtcctggc cgtgcaggcg gatcacccgg atgtgcaccc tggcctcgag
     9841 cagcctgccg tcggatgcgc ggtggaccgt ctcgaaattc gcgctgcgcc gggcaacgat
     9901 gtcccgggcc agggcctcga gctgggccgg ctggagctcg gcctggatgt ccatgacgct
     9961 gcgctggcgc agctcggctt cggtgtagcc gagtgcctgt cgcgagtagg cgttggcccg
    10021 gagaaagcgc agcgtcaccg ggtcgaccag ctcgatcgcg catggcgcct gctcgatcac
    10081 cgccttcatc agggtctcgt cgtccttctg ggcgaggcgg gtttcacgga aaccgagcag
    10141 ggcctgcgcc agttcgccga gacggtcgcc ttttttcgcc gccagtcggg tgagcgcctc
    10201 gtagtgttcc gcttcgagtt ggtcctgcgc catcctgcgc agcgcatcgg ccagtgtgtt
    10261 gcggtcattg gcgagccgct tcgccagggt gaaggcgagc aggagcgtaa gcacgatcac
    10321 ggtcaggctc gtgctcaggc tctggtcgag ccggcgctgg atgatgtgga tcgcgtcggc
    10381 gttcttctcg gctgcgtcgg ccgtcaccgc aatcgccagt tcttcggtgt ggcggacgag
    10441 cgcgttgtgg agccgatcgg cgtgggcgag gtcgagtgcg gcggcccgcg gctcgcgtgc
    10501 atcggttgcg agcaggggca gcacgagggt ccgataggac tgcaggctcg ccttggcccg
    10561 cgcatgcgac gcttgggcgg tcgaccagac gccgatcgca tcgacgcggg tctcgagtgc
    10621 ggcgagctgg ttgtcgagcc acagtgcagc aagcacctgc gcggccggat ccgggccagg
    10681 ggccacgccc ggcatgacga tgcggtgcag ttcggcatgg atgcgaccaa gcaagtcgcc
    10741 gatctcgtcc acctcggcga tctgctcgag acggagatgg gtttcgcgct cgatgtccgc
    10801 ggtggcgtcc ttcaggcgta catggcccag aaggctggtc gcgctcagga tcgccgccat
    10861 cagcaggacc ggcagcagga agtcggcgag gttgcgcagc cgatcgcgcg acacgacgtt
    10921 gatcatgggc gaggctcccg gccgctcagg cctcggcgaa gcgttgtgcg acctcggcga
    10981 aggcggggcg caattcaagg aatgcgtcca cgatgaccgg gtcgaagtgg cggccgcgtt
    11041 cggcgacgat gatccgggtc acgtcctccg gcgccaacgg ttccttgtaa acgcgccgcg
    11101 agctcagtgc gtcgaagacg tcggcgagcg ccatgaggcg cgcggaaacg ggaatctggc
    11161 ggccttgcag gcctgcgggg tagccgctgc catcccattt ctcgtgatgg ctgagcgcga
    11221 tctcgcgtgc ggtttccatg aacgcgaaag cgccccccgg caaggccccg tctccgctcc
    11281 ggctgtttgc cgcctgcgcc atggcgcggg tgatcgcatc cgcgccgatc accggatgtt
    11341 tcttcatgat ctcgaattct gcggacgtca ggcgccccgg cttgaggagg atcgagtccg
    11401 ggatgccgac cttgccgatg tcgtgcaggg gggcgctgcg gacgatgatc tcgaggcgct
    11461 cgcctgcgag cgccgcgcgg tagtcggggt gcccggccag atggtgggcg agaagctcga
    11521 cgaaggactg cgtgcgcagg atgtggtggc cggtttcgat gtcgcgcact tcggcaaggc
    11581 aggcgagcgc ggagaggctg aggtcgcgga tcagcgcgtt ctcgttcgtg cggcgggcga
    11641 cttcgcgttc gagccatgcg ttctcgtcgt gcaggcgctc gcgcgccttg gcaagctcga
    11701 tgtgggtgcg tacccgtgca agcacgatgt cgggattgaa cggcttggtg atgtaatcca
    11761 ccgcgccgag ggcgaggccg tgcgtctcgc cctccaccga gtccatcgcg gtgacgaaga
    11821 tgaccgggat gtcgcgcgtc gccgcgtcgg ccttgagggc ctgcagcacc tggtagccgt
    11881 ccatgtccgg catcatgacg tcgagcagga tcacgtcggg cagcgtgccg atggtgaccg
    11941 catgcagtgc gcgctctccc gagatggcgg cgcggacacg atagtgcggg cgcagcgtct
    12001 cgccgaggat ggtgaggttc tcgggcgtgt catcgacgat gaggacggtg cttcgtgtgc
    12061 tgctgtcggc gggcatggcc tggattccgc gggttcggcg tggccgattg tactcgggtc
    12121 ggggagtcgg gtacctgcgc ctaaatatca ataaagaaga aaacgtcaca ttcgacttta
    12181 tcgaaaattc ctttcggggt gccgcggctt agttctcggt cgagcgcgat cgaccgcccc
    12241 ggcggcgtgc cgcggcgccc ttgcgggcgt ggtcgtggcg gagttcagcc ctgcatcgtc
    12301 ccgatcgtgt ggcgcaggcg atggtgcgcc agcgccagga agaccgcgcc gatcgccgcc
    12361 accatcgcga aggccggcca caccacgtcc agcccggcgc cgcggaagag gatggactgc
    12421 gcgagcgaca cgtagtgcgt ggtcggcgcc agccccatga tggtctggat ggcctcgggc
    12481 atgctctcgc gcggggtcac gccgccggag aggatctgca gcggcagcag gatcaggatg
    12541 gacatcagcc cgaactgcgg catcgagcgt gcgatcgtgc ccatgtagat gccgatcgag
    12601 gcggcggcga agaggtagag cagggtgccg gcgccgaaga gcagcgccga cccggccacg
    12661 tcgatgccga gcacgccctt caccatcacc tgcagcgaga aggacgaggc caccagcacc
    12721 accacgccca tcgaccagat cttcgacagc atgatctcga ggggggtgac cggcatcacc
    12781 agcaggtgct cgatggtgcc gtgctcgcgc tcgcggatca gtgcggcgcc ggtgagcagg
    12841 atgccgagca tggtgacgtt gttgatgatc gcgttgatcg cggcgaacca gctcgcctcg
    12901 aggttggggt tgaacatcgc gcgcggcacg agctcgaccg gctgcggcgc cggggcgcgc
    12961 atgcgctgca cgaaggcgcg cacctcgtcg gcgaggatgt tctggatgta ggccgcgccg
    13021 gtctgcgcct ggccctggcg ggtggcgtcg atgttgagct gcagcgccgg gctgcggccg
    13081 gcgagcacgt cgcgctggaa gttcggcggg atgtcgagca cgaaggtgtg gcggccggcg
    13141 tcgaggccgc ggtccatgtc ggcgaggccg acgtgctcgg gcggcaggaa ctgcggtggc
    13201 tgcagtgcgg cgacgatgcg gccggagagc tgcgagccgt cctcgtccac caccgcgatc
    13261 gcggcgcgcg acaggctctc cggcttggcg cgcgcgtcgg cgtagatcga ggcgctgaac
    13321 atgaacacga tcaggaagac catcgcgagg tcgcgcgaca ggctgcggaa ctccttgagg
    13381 ccgaggttcc aggtgttgcg caggtgggtg ctgcggccgg cttggcccgg ctgcttcggt
    13441 ggagtcgggt ggcggaccat ggtggcgggt ctcacttggc ctgcttcttc agcagcagcg
    13501 ccga
//
LOCUS       DAOQBS010000082        13087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3831_length_13087_cov_14.901857, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000082 DAOQBS010000000
VERSION     DAOQBS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3831_length_13087_cov_14.901857"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /gene="pgi"
                     /locus_tag="PKN25_10110"
     CDS             complement(<1..1587)
                     /gene="pgi"
                     /locus_tag="PKN25_10110"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNV91297.1"
                     /translation="MPAPSSLAAWKSLQQRRRTHLPRSLRERFAADPGRAARMQASAC
                     GITLDYSKNLLDDDELVLLLRLADEADLASRIRAMFGGERINTTEVRAVLHVALRCPP
                     GAHWPVGDLDVAAEVHAVRARMRTFCLQVRGGDWRGHGGERITDVVNIGIGGSDLGPA
                     MVCEALADHAGDGPRVHFVSNVDGVHIGDVTRRCDPRSTLFVVASKTFTTQETLANAH
                     AARAWLVGALGDEAAVARHFVAVSTNAAAVRDFGIDTANMFGFWDWVGGRFSLWSAIG
                     LPIALALGPDRFDELLAGAHAMDEHFRTAPPAANLPVLMALLAIWNVNFLDAASQLVA
                     PYHQRLHRLPAYLQQLDMESNGKSVRRDGTPVDCRTSPILWGEQGINGQHAYFQLVHQ
                     GTQLVPVDFIGVLDPGREDEALHRIAFANLVAQAEALMRGKTAAEAEAEMRAAGVPEE
                     RIAALLPHRVFSGDRPSNVVLLERLDPFTLGALVAAYEHRTFVQGVIWDVNSFDQWGV
                     ELGKQLAARVLAELDPHAAAP"
     gene            1794..2366
                     /gene="pgsA"
                     /locus_tag="PKN25_10115"
     CDS             1794..2366
                     /gene="pgsA"
                     /locus_tag="PKN25_10115"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNV91298.1"
                     /translation="MPLNIPNALTWARIAMIPVFVGLFYLPESWMPIATQNLVATVIF
                     VAAAVTDWFDGYLARALGQTSAFGAFLDPVADKLMVAAALILLVQLGRVDALIAVVII
                     GREITISALREWMARVGQSASVAVAYVGKLKTTAQMVAIPMLLFDASLYGLDLRLAGE
                     VLIYVAAALTLWSMGYYLRRAMPLLRETEE"
     gene            2439..2514
                     /locus_tag="PKN25_10120"
     tRNA            2439..2514
                     /locus_tag="PKN25_10120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2472..2474,aa:Gly,seq:gcc)
     gene            2587..2660
                     /locus_tag="PKN25_10125"
     tRNA            2587..2660
                     /locus_tag="PKN25_10125"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2619..2621,aa:Cys,seq:gca)
     gene            2733..2808
                     /locus_tag="PKN25_10130"
     tRNA            2733..2808
                     /locus_tag="PKN25_10130"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2766..2768,aa:Gly,seq:gcc)
     gene            2957..4144
                     /locus_tag="PKN25_10135"
     CDS             2957..4144
                     /locus_tag="PKN25_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNV91299.1"
                     /translation="MPFTIDSTLPLPAGAVLPDYGDGGLYGFARGLRHWLHDRKAGWP
                     AVEVAPGERALVVLLVIDGLGERFLDTVGWGSALHAAKHAGLSSVCPSTTASAITTLA
                     TGVAPVEHGLNGWFIHDRRFGGVIAPLPLIRRSGEPLEAFRLLPRLFPVAPMYRHACR
                     PVTLVSPVQIAFSRFSLHHGRGAHIEPYEGLQDYVAAIVDMADALAHSGGLIHAYYPV
                     FDMLSHQHGCRSAEAVACFTRVDAAFVSLQQALAGRDVRLLVTADHGFIDAPPERRID
                     LAPDGEVAAMLAAPLFGERRLAFCRVRAGAQAEFEAWAADELRGKAVAVRGEDFLAAG
                     LLGPGQVHPRLSERLGSHALLMEAGWTIVDHVAGEHEHTMIGVHGGLSADEMRVPLML
                     ART"
     gene            complement(4229..4687)
                     /locus_tag="PKN25_10140"
     CDS             complement(4229..4687)
                     /locus_tag="PKN25_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HNV91300.1"
                     /translation="MIVLDLHCSNAHRFEGWFGSAAAFEAQLADRLVNCPICGATEVR
                     RLPSAPYVQTGQSASARPPAAGEVQAASAVPPSAPAPEGLARLMTQLRSLARVAENVG
                     ERLPEEARRIHYGETEARDIRGSASADEVESLLEEGILVLPVPPAEEDLH"
     gene            complement(4757..4841)
                     /locus_tag="PKN25_10145"
     tRNA            complement(4757..4841)
                     /locus_tag="PKN25_10145"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4805..4807),aa:Leu,seq:taa)
     gene            complement(4851..5111)
                     /gene="minE"
                     /locus_tag="PKN25_10150"
     CDS             complement(4851..5111)
                     /gene="minE"
                     /locus_tag="PKN25_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNV91301.1"
                     /translation="MSFLARLFGEKKKTAEIAKNRLSLLIAHERAGDSPRADFLPDLQ
                     RELIEVISKYVAVNPDDIKVQLEKQDNYEVLEVNIVLPEQNR"
     gene            complement(5113..5925)
                     /gene="minD"
                     /locus_tag="PKN25_10155"
     CDS             complement(5113..5925)
                     /gene="minD"
                     /locus_tag="PKN25_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNV91302.1"
                     /translation="MRVIVVTSGKGGVGKTTTSAAFASGLALRGFKTAVIDFDVGLRN
                     LDLIMGCERRVVYDLVNVVNGEARLNQALIKDKHCDNLFILPASQTRDKDALTEEGVE
                     KVLQELEHMGFEYVVCDSPAGIERGAVMALTFADEAIVTTNPEVSSVRDSDRILGILQ
                     SKSKRAREGGEPVKEHLLITRYSPKRVEDGEMLSYKDVQELLRVPLIGVIPESESVLH
                     ASNQGTPAIHLKGTDVAEAYADVVGRFLGEDLPLRYVNYEKPGLIKRLFGGK"
     gene            complement(5957..6733)
                     /gene="minC"
                     /locus_tag="PKN25_10160"
     CDS             complement(5957..6733)
                     /gene="minC"
                     /locus_tag="PKN25_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNV91303.1"
                     /translation="MSASAPARSIEFHNTTLGATIAVLQAAEPSTLADSLHKMLGGMP
                     DFFSGELLILDFGGVAQLPERIDWSGLQSLLRRYRLQPIGVMRLAAEHHEGARRAGLA
                     LVDPAQLAARPPAEAPARHAAPAEPAPAAAPPPLPPPVAAAATPTMFVERPLRSGQQI
                     YARGTDLVLLGGVSPGAEVIADGSIHCYGPLRGRAIAGAQGNQAARIVASNFGPELVS
                     IAGVYRTFERGIPEAFAGKPTLVRLNGTDHTLNLEALKLD"
     gene            complement(6830..7693)
                     /locus_tag="PKN25_10165"
     CDS             complement(6830..7693)
                     /locus_tag="PKN25_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNV91304.1"
                     /translation="MKTPLPTSYVQRFLLEDLDIRGAVVRLTDVWEAMQDGRGYAPAV
                     ARLLGEMSAVSTVIAGNLKQPGRLTFQITGHGPVSLLVIDCAETLNLRGYAKAEGTPA
                     AGTLVELVGDGRLQLSLDIEGLDQPYQSLVPLEGDSIAEVFEHYLVQSEQQPARLWLA
                     CSAQAAVALFVQKLPGADLKDIDGWSRVQQLAHTVREDELLGLDAEQILRRLFAEEDI
                     RLFDARPVTHEWPADPDKIAEMLRALGEDEVRTVLDQHGEVVVHDDLSNHTYRFDRSD
                     VDALFRPPTLH"
     gene            complement(7690..8082)
                     /locus_tag="PKN25_10170"
     CDS             complement(7690..8082)
                     /locus_tag="PKN25_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HNV91305.1"
                     /translation="MPTTIHGIRNCDTMKKTFAWFDGAGVAYQLHDYKKAGVDAATLA
                     RWCEKLGWEALVNKRGTTWRRLPPEQQAIADTAAAIALMQAQPSLIRRPVVEFADGGL
                     VVGLDTEAFERHSRGRAQPPAGTPDTPR"
     gene            complement(8093..>10456)
                     /locus_tag="PKN25_10175"
     CDS             complement(8093..>10456)
                     /locus_tag="PKN25_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNV91306.1"
                     /translation="IAEELGVSPRQVIAAVQLIDDGATVPFIARYRKEVTGGLDDTQL
                     RTLEERLGYLRELEDRRATVLASIEEQGKLTAELRAEVEDADTKQRLEDLYLPYKPKR
                     RTKAQIAREAGIGPLAETLLADPTLTPEVEAEKYINAEAGFADTKAVLDGARQILMEH
                     FAEDATLLGELRSYLHEHGRVRSSVIEGKENEGAKFRDWFDFAEPIATMPSHRALALL
                     RGRNEGVLRLALDVERPDPDAPHPCEGRIAVRFGIRSQGRPGDRWLAETARWAWSVKI
                     SIHLELELMSELRERAEEEAIRVFARNLHDLLLAAPAGTRATIGLDPGIRTGVKVAVV
                     DATGKLVDTATVYPFEPRRDRESSIATIAALAKKHAVELIAIGNGTASRETDALVAEL
                     MKRYPELQLTKIVVSEAGASVYSASELAAREFPELDVSLRGAVSIARRLQDPLAELVK
                     IDPKSIGVGQYQHDVNQGRLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNATLA
                     GNIVEYRNTRGPFRSRSALKDVPRLGPKTFEQAAGFLRIPSGDNPLDASSVHPEAYPV
                     VERILARVRKSVRELMGNGGFLKSLKPEEFTDERFGLPTVQDILAELEKPGRDPRPEF
                     RTATFREGVETLKDLEPGMLLEGVVTNVTNFGAFVDIGVHQDGLVHISALSNTFVKDP
                     HSVVKAGQVVKVKVLEVDIPRHRIALTMRLGDEVTAKRGESGARRDEGAPRRGEGGQA
                     PRSEQRPRGGDNRRPPAQGKGGARQEASRPAAANALAEAFARARGNR"
     assembly_gap    10457..10556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10859..11707)
                     /locus_tag="PKN25_10180"
     CDS             complement(10859..11707)
                     /locus_tag="PKN25_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HNV91307.1"
                     /translation="MPATIEPIEFHGQPALRLATASGARAVVALHGAQVLSWAPPGGE
                     ERLYLSPKAVFDGHSAVRGGIPVCFPQFADLGPLPAHGFARNRRWSLTTERSGKDFAL
                     AGLTLADDEATRALWPYAFSAELSILIEDSRLDLELEITNTGDEAFEFTAALHTYLAV
                     REVEESRIEGLHGFSYRDKTDADRIQRDSGDALVIESETDRVYHEVNRPLLLREYHRS
                     LGVNADGFPDVVVWNPWIERSAAIADLPDDGFRRMLCIEAAAARQPVRVQPGESWWGR
                     QTLVAL"
     gene            complement(11711..>13087)
                     /locus_tag="PKN25_10185"
     CDS             complement(11711..>13087)
                     /locus_tag="PKN25_10185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV91308.1"
                     /translation="PEPAVELFSHISAYYQNQKLLMQTPSSLSHLPPPSIESARLVIE
                     MALSERRKKLNEMESKALLAAFRIPIAQTVVARSAAEAMVLSAEIGLPVVMKIDSPNI
                     VHKSEVGGVRLNIRSLAAVRSTYQEILDEVKRVQPEAVINGIAIEPMIQKRNGRELVV
                     SVRRDPVFGPAITFGEGGNLVEENRDVAVALPPLNSFLVKDMIRSTRISTRLGEFRNM
                     PAVDMNALELVLLRISEMVCELPWITAMEINPLIVDENGVVAVDANISVENVSPTADR
                     YDHVAIHPYPSHLISTWTVPDGTTVTIRPIKPEDAELEVDFVRRLSAETKYYRFMNTM
                     RELPPAMVARLTQIDYDREMAFVATLEADGVENEIGVCRYAVNPDGESCEFAVVVADD
                     WQHRGLARKLMGVLIETARSRGIQYMNGVFLANNERMLKFVQKLGFVLSNDPEDSSVK
                     LGILALQD"
BASE COUNT         1870 a         4225 c         4726 g         2166 t
ORIGIN      
        1 gggggcggcc gcgtgcgggt cgagctcggc cagcacgcgc gcggcgagct gcttgccgag
       61 ctccacgccc cattggtcga agctgttgac atcccagatc acgccctgga cgaaggtgcg
      121 gtgctcgtag gcggcgacca gcgcgccgag cgtgaagggg tcgaggcgct cgagcagcac
      181 aacgttgctc ggccggtcgc cggagaagac gcggtgcggg agcagggcag cgatacgttc
      241 ctcgggaacg ccggccgcgc gcatctcggc ctcggcttcg gcggcggtct tgccgcgcat
      301 cagcgcttcg gcctgcgcca ccaggttggc gaaggcgatg cggtgcaggg cctcgtcctc
      361 gcggccggga tcgagcacgc cgatgaaatc caccggcacg agctgcgtgc cctgatgcac
      421 gagctggaaa taggcgtgct ggccgttgat gccttgctcg ccccacagga tggggctggt
      481 gcggcaatcc accggcgtgc cgtcgcggcg caccgacttg ccgttggatt ccatgtcgag
      541 ctgctgcagg taggcgggca ggcggtgcag gcgctgatga tagggtgcga cgagctggct
      601 ggcggcgtcg aggaaattca cgttccagat ggcgagcagc gccatcagca ccggcaggtt
      661 ggcggcaggc ggggcggtgc ggaaatgctc gtccatcgcg tgcgcgccgg cgagcagttc
      721 gtcgaagcga tcggggccga gcgcgagcgc gatgggcagg ccgatcgcgc tccatagcga
      781 gaagcgccca cccacccagt cccagaagcc gaacatgttt gcggtgtcga tgccgaagtc
      841 gcgcaccgct gcggcattgg tcgacacggc gacgaagtgg cgcgccaccg cggcttcgtc
      901 gccgagcgcg cccaccagcc aggcgcgtgc ggcgtgcgcg ttggcgagcg tctcctgcgt
      961 ggtgaaggtc ttcgaggcga ccacgaacaa ggtgctgcgc ggatcgcagc ggcgcgtcac
     1021 gtcgccgatg tgcacgccgt ccacgttgga gacgaagtgc acgcgcgggc cgtcgccggc
     1081 gtggtccgcg agcgcctcgc acaccatcgc cggaccgagg tcggagccgc cgatgccgat
     1141 gttcaccacg tcggtgatgc gttcgccgcc gtggccacgc cagtcgccac cgcgcacctg
     1201 caggcagaac gtgcgcatgc gcgcccgcac cgcatgcacc tcggcagcga cgtccaggtc
     1261 cccgactggc cagtgtgccc ccggcgggca gcgcagggcc acgtggagga cggcgcggac
     1321 ctcggtggtg ttgatgcgtt cgccgccgaa catcgcccgg atgcgactgg cgagatcggc
     1381 ctcgtcggcc aggcgcagca acagcaccag ctcgtcgtcg tcgagcaggt tcttcgagta
     1441 atccagggtg atgccgcagg cgctggcctg catgcgtgcg gcgcgcccgg gatcggcggc
     1501 gaagcgctcg cgcagggagc gcgggagatg ggtgcgacgg cgctgctgga gcgatttcca
     1561 ggcggccagg gagctggggg caggcattgg aagggaccgg aacggccgat gcgaggaaga
     1621 tggatgcttg tcgaggatct tacctagcgc gcgcgtgcct gtgtgcaccg gaagcgccgc
     1681 agggcgggac tttcggcacg ctgcgcgcgc gcacggaatg cggccgtccc tacgcgcgta
     1741 tgggcgcttg atatactcgc cgctccccga acctttgcga gtcgccctcc ggcatgccgc
     1801 tcaacattcc gaatgcgctg acctgggcgc ggatcgccat gatcccggtc ttcgtcggcc
     1861 tcttctatct ccccgagtcg tggatgccga tcgcgaccca gaacctcgtc gccacggtga
     1921 tcttcgtcgc cgccgcggtg accgactggt tcgacggcta cctcgcccgc gcgctcggcc
     1981 agacctcggc cttcggcgcc ttcctcgacc cggttgccga caagctgatg gtggccgccg
     2041 cgctgatcct gctggtgcag ctcggtcgag tggatgcgct gatcgcggtc gtcatcatcg
     2101 gtcgcgagat caccatctcg gccctgcgcg agtggatggc gcgggtcggc cagtcggcca
     2161 gcgtcgccgt ggcctatgtc ggcaagctca agaccaccgc gcagatggtc gcgatcccga
     2221 tgctgctgtt cgatgcctcc ctgtacggtc tcgacctgcg cctggccgga gaagtgctga
     2281 tctacgtcgc cgccgcgctc acgctatggt cgatggggta ctatctgcgg cgcgcgatgc
     2341 cgctgctgcg cgagacggaa gaatgaagcg gatcgcgaga aagcgcagat aggggttgac
     2401 gagctaagaa ttgtgtctat aatggcggtc tttcccgggc gggaatagct cagttggtag
     2461 agcgcaacct tgccaaggtt gaggtcgcga gttcgagact cgtttcccgc tccaaagaat
     2521 tcgcaggctg ggcagtgccc agccaagtgg ccccggggca ttggggcagc tggccgaaac
     2581 ggttgtggcg cgatggcaga gtggttatgc agcggcctgc aaagccgtgt accccggttc
     2641 gattccgagt cgcgcctcca gtagtaaaaa agttgtaaag aagtattgcg atgcggtgca
     2701 aaagaagcta gaatttgcgc cctgtcgtca aagcgggaat agctcagttg gtagagcgca
     2761 accttgccaa ggttgaggtc gcgagttcga gactcgtttc ccgctccaga ttctgaaagc
     2821 cggatgtgcg cccagcgccc atccggcttt tgcattgtgc ccccgcggtg catgcctcgc
     2881 cggcggtttg ccgggcgcgg ccttgcaggg taggctcgaa gctctcccgg agctctctgc
     2941 gatcctcgcg caccgcatgc ccttcaccat cgactccacc ttgcccctcc cggccggcgc
     3001 cgttcttccc gactatggcg acggcgggct ctacggcttc gcgcgcgggc tccgccactg
     3061 gctgcacgac cgcaaggccg ggtggcctgc ggtcgaggtc gcgccgggcg agcgcgcgct
     3121 ggtcgtgctg ctggtcatcg acgggctggg cgaacgcttt ctcgacacgg tcgggtgggg
     3181 ctccgcgctg catgcggcca agcacgccgg cctgagctcg gtgtgcccga gcaccacggc
     3241 gagtgcgatc accacgctgg cgaccggcgt cgcgccggtc gagcacggcc tcaacggctg
     3301 gttcatccac gatcgccgct tcggtggcgt gatcgcgccc ttgccgctga tccgccgcag
     3361 cggcgagccg ctggaggcct tccgcctgct gccgcgcctg ttcccggtgg cgccgatgta
     3421 tcgccacgcc tgccgaccgg tcaccctggt ctcccccgtg cagatcgcat tctcgcgctt
     3481 ctcgctgcac catgggcgcg gggcacacat cgagccttac gaagggctgc aggactacgt
     3541 ggccgccatc gtcgacatgg ccgatgcgct cgcgcacagt ggcgggctga tccacgccta
     3601 ttacccggtg ttcgacatgc tgagccacca gcacggctgc cgctcggccg aggcggtcgc
     3661 gtgcttcacg cgcgtggatg ccgccttcgt gtcgctgcag caggcgctgg cggggcgcga
     3721 cgtgcgtctg ctggtgacgg ccgaccacgg cttcatcgac gcgccacccg agcgccgcat
     3781 cgacctcgcg cccgacggcg aggtcgccgc catgctcgcc gcgccgctgt tcggcgagcg
     3841 ccggctggct ttctgccggg tgcgcgccgg tgcgcaggcc gaattcgaag cgtgggctgc
     3901 ggacgagctg cgtggcaagg cggtggcggt gcgcggcgaa gactttctcg ccgccggtct
     3961 gctcggcccg ggtcaggtgc atccgcggct gtccgaacgc ctgggcagcc acgcgctact
     4021 gatggaggcc gggtggacga tcgtggatca cgtggcgggc gagcacgagc acaccatgat
     4081 cggcgtgcat ggtggcctca gcgcggacga gatgcgcgtg ccgctgatgc tggcacgtac
     4141 ctgaggacgg gcactccaca cggctgcgac agggcggtgg acctggcggt ccgtgctcgg
     4201 cacgcatccc cgcctgcgcc ctggttcttc agtgcaggtc ttcctcggct ggcgggaccg
     4261 gcagcaccag gatgccctct tcgagcaggg actcgacctc gtcggcggag gccgagccgc
     4321 gaatgtcgcg cgcctcggtt tcgccgtagt ggatgcggcg cgcctcctcg ggcaggcgct
     4381 cgcccacgtt ctccgcaacg cgcgcaaggc tgcgcagctg cgtcatcagg cgcgcgagtc
     4441 cttcgggagc aggagcggag ggagggacgg cgctcgcggc ctgcacctca ccggctgcgg
     4501 gcggccttgc cgaggcggac tggccggtct ggacgtaggg cgcgctcggc aggcggcgga
     4561 cctcggtggc gccgcagatc gggcagttca ccaggcggtc cgcgagctgc gcctcgaagg
     4621 ctgcggcgga accgaaccag ccttcgaagc ggtgtgcatt gctgcagtgt aggtcgagta
     4681 cgatcatggc gaggactgtc cggtcactgt gccgggcagg acgccgcagc aaggatggga
     4741 caatggctga gcgggatggt gcccgggacg ggactcgaac ccgtaaggct tgcgccggcg
     4801 gattttaagt ccgctatggt taccgatttc atcacccggg cggccgaggc tcagcggttc
     4861 tgctccggca gcacgatatt gacctcgagc acctcgtagt tgtcctgctt ttcgagctgg
     4921 accttgatgt cgtcggggtt gaccgcgacg tacttcgaga tgacctcgat cagttcgcgc
     4981 tgcaggtcgg gcaggaagtc ggccctgggg ctgtcgcccg cgcgctcgtg ggcgatcagc
     5041 agcgacaggc ggttcttggc gatctccgcg gtcttcttct tttcgccgaa gaggcgggcg
     5101 aggaaggaca tgtcacttgc ctccgaacag gcgcttgatc aggccgggct tttcgtaatt
     5161 gacgtagcgc agcggcaggt cttcgccgag gaagcggccg acgacgtcgg cgtaggcttc
     5221 ggcgacgtcg gtacccttga ggtggatcgc gggcgtgccc tggttcgagg cgtgcagcac
     5281 ggactccgac tcggggatca cgccgatcag cggcacgcgc agcagctcct gcacgtcctt
     5341 gtacgacagc atctcgccgt cctcgacgcg cttgggcgag tagcgggtga tgagcaggtg
     5401 ctccttgacc ggctcgccgc cctcgcgcgc acgcttggac ttgctctgca ggataccgag
     5461 gatgcggtcg gagtcgcgca ccgaggagac ctcggggttg gtggtcacga tggcctcgtc
     5521 ggcgaaggtc agcgccatca ccgcgccgcg ctcgatgccg gccggggagt cgcacaccac
     5581 gtactcgaag cccatgtgct cgagctcctg cagcaccttc tcgacgcctt cctcggtgag
     5641 cgcgtccttg tcgcgcgtct gcgaggccgg gaggatgaac aggttgtcgc agtgcttatc
     5701 cttgatcagg gcctggttca ggcgggcctc gccattcacc acattgacca ggtcgtacac
     5761 gacgcgacgc tcgcagccca tgatgaggtc gaggttgcgc aggccgacat cgaagtcgat
     5821 gacggcggtc ttgaagccgc gcagggcgag tccggaggcg aatgccgcac tggtggtggt
     5881 cttgccgacg ccgcccttgc ccgaagttac tacgatgact ctcacggttt ccccttgtgg
     5941 atgcggtatg gctggctcag tcgagcttca gcgcttcgag gttgagtgta tggtcggtgc
     6001 cgttcagccg caccaaggtg ggcttgccgg caaaggcttc gggaatgccg cgctcgaagg
     6061 tgcggtacac gccggcgatc gatacgagtt cggggccgaa gttgctggcc acgatgcgcg
     6121 ccgcctggtt gccctgagcg cccgcgatcg cgcgtccgcg cagggggccg tagcagtgga
     6181 tgctgccatc ggcgatgact tcggcgccgg ggctcacgcc accgagcagg acgagatcgg
     6241 tgccgcgcgc atagatctgc tggccggagc gcagcgggcg ttcgacgaac atggtcgggg
     6301 tggccgcagc ggcgaccggg ggcggcagcg ggggcggcgc agccgcgggc gcgggctccg
     6361 cgggagcagc gtggcgcgcg ggcgcctcgg cgggcgggcg ggcggcgagc tgcgccggat
     6421 cgaccagtgc gagcccggcg cgccgggcgc cctcgtggtg ttccgccgcc aggcgcatca
     6481 caccgatcgg ctgcaggcgg tagcgccgca gcagcgactg caggcccgac caatcgatac
     6541 gctcgggcag ttgcgccacc ccgccgaaat cgaggatgag cagctcgccg ctgaagaagt
     6601 cgggcatgcc gccgagcatc ttgtgcagcg aatcggcaag cgtgctcggt tccgccgcct
     6661 gcaggacggc gatggtggcg cccagggtcg tgttgtggaa ctcgatcgat cttgcgggcg
     6721 cgctggcgga catgtgctga acggtcttca aaatcgagcg agtgtactta ggcgaacacg
     6781 ttcgggcaag gccggcggcg caagccggcc ggcattgccg gcttccggct caatgcaggg
     6841 tgggcgggcg gaagagcgcg tccacgtcgc tgcgatcgaa acggtaggtg tggttggaca
     6901 gatcgtcatg caccaccacc tcgccatgct ggtccaggac cgtgcgtact tcgtcctcgc
     6961 cgagtgcgcg caacatctcg gcgatcttgt ccggatcggc cggccattcg tgggtgaccg
     7021 gacgcgcgtc gaagaggcgg atgtcctcct cggcgaacag gcggcgcagg atctgctcgg
     7081 cgtcgaggcc gagcagctcg tcctcgcgca cggtgtgggc cagctgctgg acgcgcgacc
     7141 aaccgtcgat gtccttgagg tcggcaccgg gcagcttctg cacgaacagg gccaccgccg
     7201 cctgcgcgct gcacgccagc cacagcctgg cgggctgctg ctcggactgg acgagatagt
     7261 gctcgaagac ctcggcgatg ctgtcgcctt ccagcggcac caggctctgg tagggctgat
     7321 cgaggccttc gatgtcgagg gagagttgca ggcgcccgtc gccgacgagc tcgaccagcg
     7381 tgcccgcggc gggcgtgccc tcggccttgg cgtaaccgcg caggttcagg gtctcggcgc
     7441 agtcgatcac cagcaggctg acggggccgt ggccggtgat ctggaaggtc agccggccgg
     7501 gctgcttgag gttgccggcg atcacggtgg agactgcgct catctcgccg agcagtcgcg
     7561 ccaccgcggg cgcatagccg cgcccgtcct gcatggcttc ccagacgtcg gtgaggcgca
     7621 cgaccgcgcc gcggatgtcg aggtcctcga gcaggaaacg ttgcacatag ctggtgggga
     7681 ggggcgtctt catcttggag tgtccggtgt tcctgctggc ggttgggctc ggccccggct
     7741 gtggcgctcg aaggcctcgg tatccaggcc gacgacgagt ccgccgtcgg cgaactcgac
     7801 caccgggcga cggatcaggc tgggctgggc ctgcatcagc gcgatcgcgg cggccgtgtc
     7861 ggcgatcgcc tgctgctcgg gcgggagcct gcgccaggtg gtgccgcgct tgttgacgag
     7921 cgcttcccac ccgagcttct cgcaccagcg cgcgagtgtg gcggcgtcga cgccggcctt
     7981 cttgtaatcg tggagttggt aggcgacacc cgcgccgtcg aaccaggcga aggtcttctt
     8041 catggtgtcg cagttcctga tgccgtggat cgtcgtcggc atgacggcct cctcagcgat
     8101 tgccgcgggc gcgggcgaat gcttcggcga gtgcgttggc agcggcgggg cgggaagcct
     8161 cctggcgcgc gccacccttg ccctgcgcgg gcgggcgccg gttgtcaccg ccgcgcgggc
     8221 gctgctcgct acgcggggcc tgaccgcctt ccccgcgccg cggcgcaccc tcgtcgcgcc
     8281 gggcgccgga ctcgccgcgc ttcgcagtca cctcgtcgcc caggcgcatg gtgagcgcga
     8341 tgcggtggcg cgggatgtcc acctccagca ccttcacctt caccacctga cccgccttga
     8401 ccacgctgtg cgggtccttg acgaaggtgt tggacagcgc ggagatatgc accaggccgt
     8461 cctgatgcac gccgatgtcg acgaaggcgc cgaagttggt gacgttggtg accacccctt
     8521 cgagcagcat gccgggctcg aggtccttca aggtctccac gccctcgcgg aaggtcgcgg
     8581 tgcggaactc ggggcgcggg tcgcggccgg gcttttccag ttcggcgagg atgtcctgca
     8641 cggtgggcag cccgaagcgc tcgtcggtga actcctccgg tttcaggctc ttgaggaagc
     8701 cgccgttgcc catcagttcg cgcacgctct tccgcacgcg cgcgaggatg cgctcgacca
     8761 ccgggtaggc ctcggggtgc accgaggagg cgtcgagcgg gttgtcgccg ctggggatgc
     8821 gcaggaagcc cgcggcctgc tcgaaggtct tggggccgag gcggggcacg tccttcaatg
     8881 cgctgcggct gcggaagggg ccccgggtat tgcgatactc gacgatgttg ccggcgagcg
     8941 tggcgttcag acccgagatg cgtgcgagca gcggtgcaga cgcggtgttc acatccacgc
     9001 cgaccgcgtt cacgcagtct tccaccacgg cgtccaggct cttcgccagg cggccctggt
     9061 tgacgtcgtg ctggtactgg ccgacgccga tcgacttggg atcgatcttg accagctcgg
     9121 cgagcggatc ctgcaggcgg cgggcgatcg aaaccgcgcc gcgcaggctg acgtcgagct
     9181 cggggaactc gcgtgcggcg agttcggacg cggaatacac cgaggcgccg gcctccgaga
     9241 cgacgatctt ggtcagctgc agctccgggt agcgcttcat cagctcggcc accagcgcgt
     9301 cggtctcgcg cgaggcggtg ccgttgccga tggcgatcag ctcgaccgca tgcttcttcg
     9361 ccagcgcggc gatggtggcg atcgacgatt cgcggtcgcg gcgcggctcg aagggataca
     9421 cggtggcggt gtcgaccagc ttgccggtgg catccaccac cgcgaccttc actccggtgc
     9481 ggattccggg gtcgaggccg atggtggcgc gcgtgccggc gggggcggcg agcagcaggt
     9541 cgtggaggtt gcgcgcgaag acgcggatcg cctcttcctc ggcgcgctcg cgcagctcgc
     9601 tcatcagctc gagttcgagg tggatggaga tcttcacgct ccacgcccag cgcgcggtct
     9661 cggcgagcca gcggtcgccc gggcggccct ggctgcggat gccgaagcgc acggcgatgc
     9721 ggccttcgca gggatgcggg gcgtcggggt cggggcgctc gacgtcgagt gccaggcgca
     9781 gcacgccttc gttgcgcccg cgcagcagcg ccagcgcacg gtgcgagggc atggtggcga
     9841 tcggttcggc gaagtcgaac cagtcgcgga acttggcgcc ttcgttctcc ttgccctcga
     9901 tcacgctcga gcgcacccgg ccatgttcgt gcaggtagct gcgcagctcg ccgagcaggg
     9961 tggcgtcctc ggcgaagtgc tccatcagga tctggcgcgc gccgtcgagc accgccttgg
    10021 tgtcggcaaa accggcctcg gcgttgatgt acttttcggc ttcgacttcg ggcgtcaggg
    10081 tcgggtcggc gagcaatgtc tcggccagcg ggccgatgcc ggcctcgcgc gcgatctgtg
    10141 ccttggtgcg gcgcttgggc ttgtagggaa ggtagaggtc ctcgaggcgc tgcttggtgt
    10201 cggcgtcctc gacctcggca cgcagctcgg cggtgagctt gccctgctcc tcgatcgagg
    10261 ccagcacggt ggcgcggcgg tcttcgagct cgcgcaggta gcccaggcgc tcctccagcg
    10321 tgcgcaactg cgtgtcgtcc aggccaccgg tgacttcctt gcggtagcgg gcgatgaagg
    10381 gcacggtggc gccatcgtcg atgagctgga cggcggcgat gacctggcgc ggcgagacgc
    10441 cgagctcttc ggcgatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgag
    10561 ctggacggcg gcgatgacct ggcgcggcga gacgccgagc tcttcggcga tgcggtattc
    10621 gatcgggggg agcatgggga agaggcggtg aagcgcagaa gataaggccc ggatagtgca
    10681 acaggcggcg cggggagagc aacccgcgga ccggtgtggc ggccgctgcg gggcgaagcg
    10741 agtcgtgagc gggcgatccg gcgacggcgg cgcggactcc gggcgccgtg agtgcgactc
    10801 gcgtcgctcc ggcagaaggc agcgaggctg cggcggctcg tgcgtgcgcg acgcaggctc
    10861 agagggcgac cagggtctgc cgcccccacc acgattcgcc gggctgcacg cgtaccggct
    10921 ggcgggcggc ggcggcctcg atgcagagca tgcggcggaa gccgtcgtcc ggcaggtccg
    10981 cgatcgcggc gctgcgctcg atccaggggt tccacaccac cacgtcgggg aaaccgtcgg
    11041 cattgacgcc caggctgcgg tggtattcgc gcagcagcag cgggcggttt acctcgtgat
    11101 agacgcggtc ggtctcggac tcgatcacca gggcgtcccc gctatcgcgc tggatgcggt
    11161 cggcgtcggt cttgtcgcga taggagaagc cgtgcaggcc ttcgatgcgc gattcctcga
    11221 cctcgcgtac cgcgaggtag gtgtgcagcg cggcggtgaa ctcgaaggcc tcgtcgccgg
    11281 tgttggtgat ctcgagttcg aggtcgagcc ggctgtcctc gatcaggatc gacagttcgg
    11341 ccgaaaacgc gtacggccac agcgcgcggg tggcttcgtc gtcggcgagc gtcagccccg
    11401 cgagggcgaa gtccttgccg ctgcgctcgg tcgtgagcga ccaccgccgg ttgcgcgcga
    11461 agccgtgcgc gggcaggggg cccaggtcgg cgaactgcgg gaagcacacc gggatgccgc
    11521 cacggaccgc gctgtgcccg tcgaacactg ccttcgggct caggtacagg cgctcctcgc
    11581 cacccggcgg cgcccacgac agcacctgcg cgccgtgcag cgccaccacc gcgcgtgcac
    11641 ccgaagcggt ggccaggcgc agcgctggct ggccgtggaa ctcgatgggc tcgatcgtcg
    11701 cgggcatcgc tcagtcctgc agcgccagaa tgcccagctt gaccgagctg tcttccgggt
    11761 cgttgctgag cacgaagccg agcttctgca cgaacttgag catgcgctcg ttgttggcga
    11821 ggaagacgcc gttcatgtac tggatgccac ggctgcgcgc ggtctcgatc agcacgccca
    11881 tgagcttgcg ggcgaggccg cggtgctgcc agtcgtcggc caccacgacc gcgaactcgc
    11941 aggactcgcc gtcggggttc accgcgtagc ggcacacacc gatctcgttc tctacgccgt
    12001 cggcctcgag ggtggcgacg aaggccatct cgcggtcgta gtcgatctgg gtgaggcggg
    12061 cgaccatcgc gggcggcagc tcgcgcatgg tgttcatgaa gcggtagtac ttggtctcgg
    12121 ccgacaggcg gcgcacgaag tcgacctcga gctcggcgtc ctccggcttg atcggacgga
    12181 tcgtcaccgt ggtgccgtcg ggcacggtcc aggtgctgat caggtgcgac ggatacgggt
    12241 ggatcgcgac gtggtcgtag cggtccgccg tcggagacac gttttccacg ctgatgttcg
    12301 cgtcgaccgc gaccacgccg ttctcgtcca cgatcagcgg gttgatctcc atcgcggtga
    12361 tccacggcag ctcgcagacc atctcggaga tgcgcaacag caccagctcg agcgcgttca
    12421 tgtccaccgc cggcatgttg cggaactcgc cgaggcgggt ggagatgcgg gtcgagcgga
    12481 tcatgtcctt gacgaggaag ctgttgagcg gcggcagggc caccgcgacg tcgcggttct
    12541 cctcgaccag gttgccgccc tcgccgaagg tgatcgcggg gccgaacacc gggtcgcggc
    12601 gcacgctcac caccagctcg cggccgttgc gcttctggat catcggctcg atcgcgatgc
    12661 cgttgatcac cgcctcgggc tgcacgcgct tgacctcgtc gaggatctcc tgataggtcg
    12721 agcgcaccgc ggcgaggctg cggatgttca ggcgcacgcc gcccacctcg gacttgtgca
    12781 cgatgttcgg cgagtcgatc ttcatcacca ccggcaggcc gatctcggcc gagagcacca
    12841 tcgcctcggc ggcactgcgc gcgaccacgg tctgcgcgat cggaatgcgg aaggccgcga
    12901 gcagcgcctt cgactccatc tcgttgagct tcttgcggcg ctcggacagg gccatctcga
    12961 tcaccaggcg cgcgctctcg atcgagggcg gcggcaggtg cgacagcgag gacggcgtct
    13021 gcatcagcag cttctggttc tggtagtagg ccgagatgtg gctgaagagc tcgaccgccg
    13081 gctccgg
//
LOCUS       DAOQBS010000083        13083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3836_length_13083_cov_14.079214, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000083 DAOQBS010000000
VERSION     DAOQBS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 13083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13083
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3836_length_13083_cov_14.079214"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..665)
                     /locus_tag="PKN25_10190"
     CDS             complement(219..665)
                     /locus_tag="PKN25_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV91309.1"
                     /translation="MMTTVKQILEAKGSTFHAVRPTDSVFDALSLMAQFDIGCVLVTD
                     SDKLVGIFTERDYARKVVLKGLVSRDVKVGDLMTPNPYTVGLTGTADDVMATMTAKRF
                     RHIPVVEEGKVLGIVTIGDMVKSIVTQHEKTIKQLESYIAGDLAAE"
     gene            complement(799..1626)
                     /gene="panC"
                     /locus_tag="PKN25_10195"
     CDS             complement(799..1626)
                     /gene="panC"
                     /locus_tag="PKN25_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNV91310.1"
                     /translation="MQIHENIDSLRAARAGAGRVAFVPTMGNLHEGHIALMRQAREHA
                     DCVIASIFVNRLQFGAGEDFDRYPRTFAADCEKLAAAGVEHLFAPDESVMYPQPQRYH
                     VDPPAEQVSILEGEFRAGHFRGVATVVMKLLNIVRPEVALFGKKDYQQLMVLSNMVRE
                     LAMPIEVVPGETVRAEDGLALSSRNGYLSADERARAPLLHHLLAGIRAAVRTGERDFA
                     RLEADAMARLSAAGWAPDYVAVRRRLDLQPPVQEDDVLVVLAAAKLGRTRLIDNLEI"
     gene            complement(1656..2471)
                     /gene="panB"
                     /locus_tag="PKN25_10200"
     CDS             complement(1656..2471)
                     /gene="panB"
                     /locus_tag="PKN25_10200"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNV91311.1"
                     /translation="MSYLQDQKPVTLFELGRMRAEGRKIAMLTAYDASFAALLGRAGV
                     DSILIGDSLGNVLQGQKSTLPVTLEQMAYHTECVARAGARPFVVTDMPFGSYHESREQ
                     AMRNAARLMAAGAQMVKLEGGEFMAETVRFLVERGVPVCAHIGLTPQAVHQLGGYRVQ
                     GRSEEGAARLKADALVLEQAGAALMVMEMVPATLAGEITASLSSMATIGIGAGAACDG
                     QVLVLHDLLGVFPGKTARFVRNFMEGAASIEEAVARYAAAVKDGSFPAPEHCY"
     gene            complement(2584..3222)
                     /locus_tag="PKN25_10205"
     CDS             complement(2584..3222)
                     /locus_tag="PKN25_10205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNV91312.1"
                     /translation="MLDKARYIVVEGPIGAGKTSLARRLADRLQAEAVFEQAELNPFL
                     GRFYQQSERWGLATQLSFLFQRIDQLSTLGPIEGERRVVADFLFDKDHLFAEQNLSPD
                     ELALYRRVHDGLQPANAPRPDLVIYLQARPDTLMERIRKRGLDAERRITELYLEQVAE
                     RYTRYFYQYDAAPLFIVDAGKLNPVDRDEDFELLLERLREMRSYREFFGYAD"
     gene            complement(3251..3622)
                     /locus_tag="PKN25_10210"
     CDS             complement(3251..3622)
                     /locus_tag="PKN25_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNV91313.1"
                     /translation="MAAWIQSVSSIPVWMQTALLLTASNVFMTFAWYGHLKNMQTKAW
                     YVAALVSWGIALFEYLLQVPANRIGATTLSLAQLKIMQEVITLLVFVPFVVLYMKQPL
                     KLDYLWAALCMLGAVYFIFRN"
     gene            complement(3656..4162)
                     /gene="folK"
                     /locus_tag="PKN25_10215"
     CDS             complement(3656..4162)
                     /gene="folK"
                     /locus_tag="PKN25_10215"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNV91314.1"
                     /translation="MVRAYVAFGANLGDPAAAFFEAVQRLDALPGTRVVARSSLYRSA
                     PVGVAGQPDYLNAVIALDTALAPRPLLEALLAIEHDSGRRRDFPMAPRTLDLDLLLHG
                     DAVIDEPGLQVPHPRMHERAFALLPLAEIDPAASIPGRGAVAALLPALAGQAIERVPT
                     PADSARHP"
     gene            complement(4173..5543)
                     /gene="pcnB"
                     /locus_tag="PKN25_10220"
     CDS             complement(4173..5543)
                     /gene="pcnB"
                     /locus_tag="PKN25_10220"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNV91315.1"
                     /translation="MIRKLLRKVFKRPATPARHEPALIPVERHGIRREQLSPAARKVC
                     AVLQEKGFKAFVVGGAVRDLLIGHPPKDYDVATSATPEEVRAAFRRSRIIGRRFKIVH
                     VMSGPETIEVSTFRASQDAESADTDEHGRVLRDNVFGNQQEDATRRDFTVNALYYDPG
                     TETIVDYHHGVADIQQKTLRMIGDPRTRYREDPVRMLRAVRLAAKLGLTIDPAARQPI
                     REMGPLLENVPAARLFDEMLKLLFSGHSLKCLQQLRAEGLHHGLLPLLDVILEQPMGE
                     RFVTLALENTDERVREDKSVSPGFLFSTLLWHEVLKTWDNKKAAGDHSQPALFEAMDE
                     VLDAQAGKLAITRRIAGDIKDIWALQPRFDKRAGKAPYRVIEQPRYRAGWDFLRLRAQ
                     AGEIPMEIVDWWDDFAHAGPDQREALLRQAPPDAAPAAKKRRRRKRKPAGETPAAADA
                     AVVDPS"
     assembly_gap    5740..5839
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6067..6729)
                     /locus_tag="PKN25_10225"
     CDS             complement(6067..6729)
                     /locus_tag="PKN25_10225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV91316.1"
                     /translation="MNLVLFDLDNTLLAGDSDFAWAQFLIGKGVLDREVQEAKNIAFY
                     EQYKAGTLDIFEFLDFQLAPLARHPRAQLDAWHCEFMAQSIRPMITAKARTLVREHLD
                     SGALVAVVTATNAFVTGPIVREFGIPHVVATIPAQQDGAFTGKPRGMPAFKAGKIERV
                     DAWLESLGLHMGSFEHSWFYSDSHNDLPLMQRVTDPVAVDPDDTLRAHAEAAGWPVIS
                     LR"
     gene            complement(6809..7501)
                     /gene="hda"
                     /locus_tag="PKN25_10230"
     CDS             complement(6809..7501)
                     /gene="hda"
                     /locus_tag="PKN25_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HNV91317.1"
                     /translation="MKQLVLDIRPDAPPTLENFVAGANAELVATVSLLASPATAEQLP
                     ARHIYLWGAPGSGRSHLLRAAVDAARAAGRPAHLLAAATVEDHLPETPGALLAIDDAD
                     QLGAAAQIALFNAFNRARANGQSLLLCGPAAPLGLALREDLRTRIGQSLIYEVKPLDD
                     DARAEILATLAARRGLRLADEVVDFLLRHGRRELSSLREVLDALDRASLEHKRPITLP
                     LLRDMIQQGLEI"
     gene            complement(7498..8604)
                     /locus_tag="PKN25_10235"
     CDS             complement(7498..8604)
                     /locus_tag="PKN25_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNV91318.1"
                     /translation="MKPPRVDRLQTVAWTATGAALVALLWLLGPILTPFVVGAVFAYI
                     CDPAVNWMVARRVPRALAVLLVILALGLLLIALALILVPMVYREGVLLVRRLPELVQM
                     FNLNVAPLLEARLGVDIKLNAEQFQQLIADNWTSAQELVPAVLAHLKTGGMAVLGFLA
                     NVVLIPLVMFYLLQEWPRILDELERIVPRPWVDGTKRVLGDIDSVMSEFLRGQLSVML
                     LLAVFYSAGLWLAGLNFWLPVGVLTGLLVFIPYVGFGGGLILAIVAALLQAQGWPPLA
                     GVAIVYALGQVVESFVLTPYLVGERIGLHPLAVIFALMAFGQLFGFVGVLVALPVSAA
                     LLVGLREVREAWLASPVYLGTQPRPIIASERERP"
     gene            8746..9813
                     /gene="purM"
                     /locus_tag="PKN25_10240"
     CDS             8746..9813
                     /gene="purM"
                     /locus_tag="PKN25_10240"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNV91319.1"
                     /translation="MADHHPEPLDPQPSLSYRDAGVDIEAGDALVERIKPFAKRTMRP
                     EVLGGIGGFGALFELSKKYKEPVLVSGTDGVGTKLKLAFQLDKHDTVGQDLVAMSVND
                     ILVQGAEPLFFLDYFACGKLDVDTAADVVQGIARGCELAGCALIGGETAEMPGMYPEG
                     EYDLAGFAVGVVEKADIIDGSRIVPGDVVLGLASSGAHSNGYSLIRKIVERAGTDMMA
                     PFHGRPFRDVVMEPTRIYVKPMLELMRAMPGVVKGMAHITGGGITENVPRILREELTA
                     RIDGDTWALPQMFRWLQFAGGVNQDEMYRVFNCGIGMVVIVSAAEAEAAEAKLAAAGE
                     TVYRIGVIDTRREGEAQTIID"
     gene            9947..10351
                     /locus_tag="PKN25_10245"
     CDS             9947..10351
                     /locus_tag="PKN25_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HNV91320.1"
                     /translation="MGYRLAADAVLLLHLGFIAFAVLGGLLALRWRLAPWLHLPAVAW
                     AAWIEASGGLCPLTPLENRLRVLAGQSGYAGGFIEHYLLPLIYPAALTRELQFALAGF
                     VVLVNLAVYAWVWRRKLRQGSTGACARRARAP"
     gene            complement(10605..11171)
                     /locus_tag="PKN25_10250"
     CDS             complement(10605..11171)
                     /locus_tag="PKN25_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91321.1"
                     /translation="MTNPSLPDPARDEAQALFTPPQPGVPALRSDRLWQLDGWTARVI
                     KNEDDDGWAVEMLQDGEAEPALVGPWTMGRDKKNPKPLDQSAFNTLVKTAGEVLQRHR
                     QQAHAMLHKRLTVFALDAQWDVRLDITPDEYEPYATLSAFDALGEEVAQVRVPPDFRL
                     GVASATAWITGGFARPTQATRGFEGWSE"
     gene            11576..12253
                     /locus_tag="PKN25_10255"
     CDS             11576..12253
                     /locus_tag="PKN25_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91322.1"
                     /translation="MPKTVPKQSDSQVPVAAAPAGAPIGIGEGLFSPNAGADGKTHFR
                     ASIDLATDGHPASLLIDNPGETGTVQGGGTSVCLTHPLKVKFEHLKDYLGTQGATVPE
                     SLSNVMERAELDCAAFYYRDSGPMLLSIALKCSDEQKGGLIAALTNSPELGKLFDVEG
                     VSLRVLKCPEACQEELETYWKAVSALPSSPSEEKDPKDVNAPPAPTAEEGGEQGAGAA
                     DTPAAVG"
     gene            12288..>13083
                     /locus_tag="PKN25_10260"
     CDS             12288..>13083
                     /locus_tag="PKN25_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91323.1"
                     /translation="MSTTEDAGPATPTTVELELELRVGGRPLALRGTLTGGGLTGLAG
                     TLRGEKTDTLGGLLAGLGEAFAGPGAALDALGGGALAAIHFEAVELAYRSTATPCVVL
                     NTTLGVGVGHTRIVVAKLLPVPGRAEDEGGLVAGFELELEPRPIGDKGLSELIGGIVV
                     ERLGVFHASRDCSGLHLEPATAPGAFAPLVERAGEGRSLHAGLNLSGRVLIGGVDLLE
                     GFGHDTGNTLDAQAPAAEPAPAAAPALASPAPEAPLPARGKAFWKRI"
BASE COUNT         1943 a         4257 c         4751 g         2032 t
ORIGIN      
        1 ctcgaccaca gcaatcgcga cttgcgtcgc tcccacagcg cggctcgaaa gccgaggggt
       61 ttctgtggga gcgacgcaag tcgcgatgag cgcgcttccg ggccgcgctg gcgcagcctt
      121 cccgcgcgcc ccgcgacgca tcccgcagac gacgacgccg gacccgactc ccgtcggctc
      181 cggcgtctgc ttgttcggcg gcgatgtcgc cgcgcggctc actccgcggc gaggtcgccg
      241 gcgatgtagc tctcgagctg cttgatggtc ttctcgtgct gggtgacgat ggacttcacc
      301 atgtcgccga tcgtcacgat gccgagcacc ttgccttcct cgaccaccgg gatgtggcgg
      361 aagcgcttgg cggtcatcgt cgccatcacg tcgtcggccg tgcccgtcag gccgaccgtg
      421 taagggttgg gcgtcatcag gtcgccgacc ttcacatcgc gcgacaccag ccccttgagc
      481 acgaccttgc gggcatagtc gcgctcggtg aagatcccga ccagcttgtc gctgtcggtg
      541 accagcacgc agccgatgtc gaactgcgcc atcagcgaca gcgcgtcgaa gaccgagtcg
      601 gtcgggcgca ccgcatggaa ggtgctgcct ttcgcctcga ggatctgttt taccgtcgtc
      661 atcatctttc cactccccct tcttcgttga aatgcgcgcc gcaggtcgcg gccgggcctt
      721 tatttgcggg ttttacggtt tttaaccaga tccgtccgcc gatgcaagcg cgggcgaacc
      781 cggatccagc ctcccgcttc agatttccag gttgtcgatc agccgggtgc ggccaagttt
      841 cgccgcggcg agcacaacga gcacgtcgtc ttcctgcacc ggcggctgca ggtcgaggcg
      901 gcggcgcacg gcgacgtagt cgggcgccca tcctgctgcg ctcaagcggg ccatcgcgtc
      961 tgcctccagc cgcgcgaagt cgcgctcgcc ggtgcgcacc gcggcgcgga tgcctgcgag
     1021 caggtgatgc agcaggggcg cgcgcgcgcg ctcgtccgcg ctcaggtagc cgttgcgcga
     1081 ggacagcgcg aggccgtcct cggcacgcac cgtctcaccc ggtacgacct cgatcggcat
     1141 cgccagttcg cgcaccatgt tcgacagcac catgagttgc tggtagtcct tcttgccgaa
     1201 cagcgcgacc tcggggcgta cgatgttgag gagcttcatg accacggtgg cgacaccgcg
     1261 gaaatggccg gcgcggaact cgccctccag gatcgacacc tgctctgcgg gaggatcgac
     1321 gtggtagcgc tgcggctgcg ggtacatgac cgactcgtcg ggcgcgaaca ggtgctcgac
     1381 tcccgcggcg gcgagtttct cgcagtcggc ggcgaaggtg cgcggatagc ggtcgaagtc
     1441 ctcgccggcg ccgaactgca ggcggttgac gaagatgctc gcgatcacgc agtcggcgtg
     1501 ttcgcgcgcc tggcgcatca gggcgatatg gccctcgtgg aggttgccca tggtgggcac
     1561 gaaggcgacg cgtccggcgc ccgcgcgtgc ggcgcgcagg ctgtcgatgt tttcgtggat
     1621 ctgcatttgc ggttcctcgc gtgcgcgacg cgggctcagt aacagtgctc gggagcgggg
     1681 aagctgccgt ccttgaccgc ggccgcgtag cgcgcgaccg cttcctcgat gctcgccgcg
     1741 ccttccatga agttgcgcac gaagcgcgcc gtcttgccgg ggaacacgcc gagcaggtcg
     1801 tgcagcacca gcacctggcc gtcgcaggcg gcgcctgcgc cgatgccgat cgtcgccatg
     1861 ctggagaggc tggcggtgat ctcgccggcg agcgtggcgg gcaccatttc catgaccatc
     1921 agtgcggcgc cggcctgctc gagcacgagc gcgtcggcct tgaggcgcgc cgcgccttcc
     1981 tcgctgcggc cctgcacgcg atagccgccg agctgatgca ccgcctgcgg ggtgaggccg
     2041 atgtgcgcgc acaccggcac gccgcgctcg acgaggaagc gcacggtctc ggccatgaac
     2101 tcgccgcctt cgagcttgac catctgcgcc ccggcggcca tcaggcgggc ggcgttgcgc
     2161 atggcctgct cgcggctctc gtggtagctg ccgaagggca tgtcggtgac gacgaagggg
     2221 cgcgccccgg cacgggccac gcattcggtg tggtaggcca tctgctcgag ggtgaccggc
     2281 agggtggatt tctgtccctg caggacgttg ccgagcgagt cgccgatcag gatggaatcg
     2341 acgccggcgc ggccgaggag cgcggcgaag ctcgcgtcat aggcggtgag catcgcgatc
     2401 ttgcgccctt cggcgcgcat cctgccgagt tcgaagaggg tgacgggctt ctggtcctgg
     2461 agatagctca ttcatcgctc ctgttgggag gcggcagtgt cggccaaact ggtgcattgc
     2521 acaagcctcg cggcccgtgc cgggcacggg cagccatcgt cggcgcgcgc ccgccgactg
     2581 cgctcaatcc gcgtagccga agaactcgcg gtagctgcgc atctcgcgca ggcgttcgag
     2641 cagcagttcg aagtcctcgt cgcggtcgac cgggttgagc tttccggcat ccacgatgaa
     2701 gagcggtgcg gcgtcgtact ggtagaaata gcgcgtgtag cgctcggcca cctgctcgag
     2761 gtagagctcg gtgatgcggc gctcggcatc caggccgcgc ttgcggatgc gctccatcag
     2821 ggtgtcgggg cgcgcctgca ggtagatcac caggtcggga cggggcgcgt tggcgggctg
     2881 caggccgtca tgcacgcggc gatagagcgc gagttcgtcg ggcgacaggt tctgctcggc
     2941 gaagaggtgg tccttgtcga acaggaagtc ggccaccaca cgacgctcgc cctcgatggg
     3001 gccgagcgtg gagagctggt cgatgcgctg gaacaggaag gagagctggg tcgccaggcc
     3061 ccagcgctcg gactgctggt agaagcggcc gaggaagggg ttgagctcgg cctgctcgaa
     3121 caccgcctcg gcctggaggc ggtcggccag acggcgggcg agggaggtct tgccggcgcc
     3181 gatcgggcct tcgacgacga tgtagcgggc cttgtcgagc atgctgggct gcctttccgg
     3241 tgagcgtgcg tcagttgcgg aagatgaaat acaccgcgcc gagcatacac aaggcggccc
     3301 acaggtagtc aagcttgagc ggctgcttca tgtagagcac gacgaagggc acgaagacca
     3361 gcagggtgat cacctcctgc atgatcttga gctgggcgag gctgagcgtg gtggcgccga
     3421 tgcggttggc gggcacctgc agcaggtatt cgaagagcgc gatcccccag ctcaccagtg
     3481 cggccacata ccaggctttc gtctgcatgt tcttcaggtg gccgtaccac gcgaaggtca
     3541 tgaacacgtt ggaggcggtg agcagcagcg cggtctgcat ccacacggga atcgagctga
     3601 cggactggat ccaggcggcc atgggcggac ggagggcggg tcggtggcga gcgggtcagg
     3661 ggtggcgggc gctgtcggcg ggggtgggca cgcgttcgat cgcctggccg gcgagcgcgg
     3721 gcagcagcgc cgcgaccgcg ccgcggccgg gaatgctggc ggcgggatcg atctcggcca
     3781 gcggcagcag cgcgaaggcg cgctcgtgca tgcgcgggtg gggcacctgc aggccgggct
     3841 cgtcgatcac cgcgtcgccg tgcagcagca ggtcgaggtc cagcgtgcgc ggcgccatcg
     3901 ggaagtcgcg gcggcggccg ctgtcgtgct cgatcgcgag cagggcctcc agcagcgggc
     3961 gcggcgcgag cgcggtgtcg agcgcgatca ccgcgttgag gtagtcgggc tggccggcca
     4021 cgcccaccgg ggcgctgcgg tacagcgagg agcgcgcgac gacgcgcgtg ccgggcagcg
     4081 catcgaggcg ctgcacggct tcgaagaacg cggcggccgg gtcgccgagg ttggcgccga
     4141 aggcgacgta tgcgcggacc aagctcgagc gctcaggacg gatcgacgac ggcggcgtcc
     4201 gccgccgcgg gggtctcgcc ggccggcttg cgcttgcgcc ggcggcgctt cttcgccgcg
     4261 ggtgcggcgt cgggcggcgc ctggcgcagc agggcctcgc gctggtcggg gcccgcgtgg
     4321 gcgaagtcgt cccaccagtc gacgatctcc atcgggatct cgccggcctg cgcgcgcagg
     4381 cgcaggaaat cccagccggc gcggtagcgc ggctgctcga tgacgcggta gggcgccttg
     4441 ccggcacgct tgtcgaagcg cggctgcagc gcccagatgt ccttgatgtc gccggcgatg
     4501 cggcgggtga tggcgagctt gccggcctgg gcgtcgagca cctcgtccat ggcctcgaag
     4561 agtgcgggct ggctatggtc gccggcggcc ttcttgttgt cccaggtctt cagcacctcg
     4621 tgccagagca gggtggagaa caggaagccg ggcgaaacgg acttgtcctc gcgcacgcgc
     4681 tcgtcggtgt tctccagtgc cagggtgacg aagcgctcgc ccatcggctg ctcgaggatc
     4741 acgtccagca gtggcagcag gccgtggtgc aggccttcag cgcgcagctg ctgcaggcac
     4801 ttcagcgagt gaccggagaa cagcagcttg agcatctcgt cgaagagccg cgccgccggc
     4861 acgttctcga gcagcgggcc catctcgcgg atcggctggc gcgctgcggg gtcgatggtc
     4921 agcccgagct tggccgccag gcgcaccgcg cgcagcatgc gcaccgggtc ctcgcggtag
     4981 cgcgtgcgcg ggtcgccgat catgcgcagg gtcttctgct ggatgtcggc gacgccgtgg
     5041 tggtagtcga cgatggtctc ggtgccggga tcgtagtaca gcgcgttaac cgtgaagtcg
     5101 cgccgggtgg cgtcttcctg ctggttgccg aagacgttgt cgcgcagcac gcggccgtgc
     5161 tcgtcggtgt cggcgctctc ggcgtcctgg ctggcgcgga aggtggagac ctcgatggtc
     5221 tccggcccgc tcatcacgtg cacgatcttg aagcgccggc cgatgatgcg cgaacggcgg
     5281 aaggcggcgc gcacctcctc gggtgtggcg ctggtggcga cgtcgtagtc cttgggcggg
     5341 tggccgatca gcaggtcgcg caccgcgccg ccgacgacga aggccttgaa gcccttctcc
     5401 tgcagcaccg cgcatacctt gcgcgcggcc ggcgacagct gctcgcggcg gatgccgtgg
     5461 cgttcgacgg gaatgagggc gggttcgtgg cgtgcggggg tggccgggcg cttgaaaacc
     5521 ttgcgcagca gcttgcggat catcgggaaa ggagtcgtgc ttgcataagc gcgcaatgat
     5581 aaccgatcga aacgtccgta cgctgcgcga aggcggaggg gagcggtggg gattcggcaa
     5641 aatggcactc ccgactcgcg tcccgcctgc ggaaaggctg cgcgcttccg gggcggctgc
     5701 gatcgcgact tgcgtcgctc ccacgggaaa ccggtgcgtn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnng cgatcgcgac ttgcgtcgct cccacgggaa accggtgcgt
     5881 cttcggggtg gtggcgatcg cgacttgcgt cgctcctacg cgaaccgtcg cgcacccgga
     5941 gccgcgatgt gggagcgacg caagtcgcga tcggcggtgc gggaggcgat gagcgtcggc
     6001 gggtgcgggg cggtgtagga gcgacgcaag tcgcgaatct tcagcgcagc gagatcaacg
     6061 accagttcag cgcagcgaga tcaccggcca gccggcggcc tcggcatggg cgcgcagggt
     6121 gtcgtcgggg tcgaccgcga ccgggtcggt gacgcgctgc atcagcggca ggtcgttgtg
     6181 cgaatcgctg tagaaccagc tgtgttcgaa gctgcccatg tgcaggccga gcgactccag
     6241 ccaggcgtcg acgcgctcga tcttgcccgc cttgaacgcc ggcatgccgc gcggcttgcc
     6301 ggtgaaggca ccgtcctgct gtgcggggat ggtggccacc acgtgcggga tgccgaactc
     6361 gcgcacgatc gggccggtga cgaaggcgtt ggtggcggtg accaccgcga ccagggcgcc
     6421 gctgtcgagg tgctcgcgca ccagcgtgcg cgccttggcg gtgatcatcg gacggatcga
     6481 ctgcgccatg aactcgcagt gccaggcgtc gagttgcgcg cgcgggtggc gggcgagggg
     6541 ggcgagctgg aagtcgagga attcgaagat gtcgagcgtg ccggccttgt attgctcgta
     6601 gaacgcgatg ttcttcgctt cctgcacttc gcggtcgagc acgcccttgc cgatgaggaa
     6661 ctgcgcccag gcgaagtcgg agtcgccggc gagcagggtg ttgtcgaggt cgaagaggac
     6721 gagattcacg atcgggctcc gcaagatgga tcggttggat gaggattcga agagggcgtg
     6781 acgcgcgtcg gacggcggcc ccgcggggtc agatttccag cccttgctgg atcatgtcgc
     6841 gcagcagcgg cagggtgatc gggcgcttgt gctcgagcga ggcgcggtcg agcgcgtcca
     6901 gcacctcgcg caggctggag agctcgcggc ggccgtggcg cagcagaaaa tccaccacct
     6961 cgtcggccag gcgcaggccg cgccgcgccg ccagggtggc gaggatctcc gcgcgcgcgt
     7021 cgtcgtcgag cggcttcacc tcgtagatca ggctctggcc gatgcgggtg cgcaggtcct
     7081 cgcgcagcgc caggccgagc ggggcggccg ggccgcacag cagcagcgac tggccgttcg
     7141 cgcgggcgcg gttgaaggcg ttgaagagcg cgatctgcgc ggcggcgccg agctggtccg
     7201 cgtcgtcgat cgcgagcagt gcgcccggcg tctcgggcag gtggtcctcg acggtcgcgg
     7261 cggcgagcag gtgggcgggc cggccggcgg cgcgcgcggc gtcgaccgcg gcgcgcagca
     7321 ggtggctgcg tccgctgccc ggcgcgcccc acaggtagat gtggcgcgcc gggagctgtt
     7381 cggcggtggc gggcgaggcg agcagcgaga cggtggcgac cagctcggcg ttggcgccgg
     7441 cgacgaagtt ctcgagcgtg ggcggggcgt cggggcggat gtcgaggacg agctgcttca
     7501 tgggcgctcg cgctcgctcg cgatgatcgg ccgcggctgc gtgccgagat acaccgggct
     7561 ggcgagccag gcctcgcgca cctcgcgcag gcctaccagc agcgcggcgc tcaccggcag
     7621 cgcgaccagc acgccgacga agccgaagag ctggccgaag gccatcagcg cgaagatcac
     7681 cgcgagcggg tgcaggccga tgcgttcgcc caccaggtag ggcgtgagca cgaagctctc
     7741 gacgacctga ccgagcgcat acacgattgc cacgccggcc agcggcggcc agccctgcgc
     7801 ctgcagcagc gcggcgacga tggcgaggat cagcccgccg ccgaagccga cgtaggggat
     7861 gaagaccagc aggccggtga gcacgcccac cggcagccag aagttgagcc cggccagcca
     7921 caggccggcg ctgtagaaca ccgccagcag cagcatcacc gagagctggc cgcgcaggaa
     7981 ctcggacatc accgagtcga tgtcgccgag gacgcgcttg gtgccgtcga cccaggggcg
     8041 cggcacgatg cgctcgagct cgtcgaggat gcgcggccac tcctgcagca ggtagaacat
     8101 caccagcgga atcagcacca cgttggcgag gaagccgagc accgccatgc ccccggtctt
     8161 gaggtgggcg agcaccgccg gcaccagttc ctgcgcgctc gtccagttgt cggcgatcag
     8221 ctgctggaac tgttcggcgt tgagcttgat gtctacgccg aggcgggcct cgagcagcgg
     8281 cgcgacgttg aggttgaaca tctgcaccag ctcgggcagg cggcgcacca gcagcacgcc
     8341 ctcgcgatag accatcggta ccaggatcag cgcgagcgcg atcagcagca ggccgagcgc
     8401 gaggatcacc agcagcaccg ccagtgcccg cggcacgcgg cgcgcgacca tccagttgac
     8461 cgccgggtcg cagatgtagg cgaacaccgc ccccaccacg aagggggtga ggatgggtcc
     8521 gagcagccag agcagggcga cgagggcggc gccggtcgcg gtccaggcga cggtttgcag
     8581 gcgatcgacg cggggaggct tcatttaaag taaaatcgcg ggcttggcgc gggcgaaaac
     8641 cgcccgcaga acacgtaaat gtagccactt ggcgttgcgg cctcaagctc ggtccctccg
     8701 ggcatgcggc gacgcccggc agcctgcgag cccgaaggaa cgatcttggc cgaccatcat
     8761 cccgaaccgc tggatcccca gccctccctt tcctaccgcg acgccggcgt ggacatcgaa
     8821 gccggcgacg cgcttgtcga gcgcatcaag cccttcgcca agcgcaccat gcgccccgag
     8881 gtgctgggcg gcatcggcgg cttcggcgca ctcttcgagc tgtcgaagaa gtacaaggag
     8941 ccggtgctgg tgtccggcac cgacggcgtc ggcaccaagc tcaagctcgc cttccagctc
     9001 gacaagcacg acaccgtcgg ccaggacctg gtggcgatga gcgtgaacga catcctggtg
     9061 cagggcgccg agccgctgtt cttcctcgac tacttcgcct gcggcaagct cgacgtcgac
     9121 accgccgccg acgtggtcca gggcatcgcc cgcggctgcg agctcgccgg ctgcgcgctc
     9181 atcggcggcg aaaccgccga gatgcccggc atgtaccccg agggcgaata cgacctcgcc
     9241 ggcttcgcgg tcggcgtggt cgagaaggcc gacatcatcg acggcagccg gatcgtcccc
     9301 ggcgacgtgg tgctcggcct ggcctcgagc ggcgcgcact ccaacggcta ctcgctgatc
     9361 cgcaagatcg tcgagcgcgc cggcaccgac atgatggcgc ccttccacgg ccgtcccttc
     9421 cgcgacgtgg tcatggaacc cacccgcatc tacgtcaagc ccatgctcga gctcatgcgc
     9481 gcgatgcccg gcgtggtcaa gggcatggcc cacatcaccg gcggcggcat caccgaaaac
     9541 gtgccgcgca tcctgcgcga ggaactcact gcgcgcatcg acggcgacac ctgggcgctg
     9601 ccgcagatgt tccgctggct gcagttcgcc ggcggggtga accaggacga gatgtatcgc
     9661 gtgttcaact gcggcatcgg catggtggtg atcgtgtcgg cggccgaagc cgaggccgcc
     9721 gaggcgaagc tcgccgccgc gggcgagacc gtgtatcgca tcggcgtcat cgacacccgc
     9781 cgcgaaggcg aggcgcagac catcatcgac tgaggatgtg ccccggcgcg cagggggcga
     9841 cccgtgcgcg ccgcgttatc gacctcggtc gagccgcgtt tccgcgccgc tttccgcgtt
     9901 cccctccgcg ttccgttcca tccccaggcc tggagctcgt cgcgccatgg gctatcgcct
     9961 cgccgccgac gcggtgctgc tgcttcacct gggcttcatc gccttcgccg tgctgggcgg
    10021 gctgctcgcg ctgcgctggc ggcttgcccc ctggctgcat ctgcccgcgg tcgcctgggc
    10081 cgcgtggatc gaagcgagcg gcggcctgtg tccgctcacc ccgctcgaga accgcctgcg
    10141 cgtgctggcc ggccagtccg gctatgcggg cggcttcatc gagcactatc ttcttccgct
    10201 gatctatccc gccgcgctca cccgcgagct gcagttcgcg ctcgccggct tcgtcgtgct
    10261 cgtcaacctg gcggtgtatg cgtgggtgtg gcgtcggaag ctgcgccagg gcagcactgg
    10321 tgcgtgcgcc aggcgggcgc gggcgccgtg atcgcgactt gtgtcgccca cgtcgccgct
    10381 ccggaagcgc agcggttttc cgtaggagcg acgcaagtcg cgatcgcagc cgctgcgggc
    10441 ctgccgacgc tcatcgactt tcgcaccgcc gatcgcgact tgcgtcgctc ctacatgacc
    10501 actacgcctg ccgagactcc cgcaggagcg atgcaagtcg cgatcggcgg ttcgggcccg
    10561 atccgcatcg ggagtgaaca gccgggtacg gtgcgcttgc ccggctactc gctccagcct
    10621 tcgaaaccgc gcgtggcctg tgtcgggcgg gcgaagccgc cggtgatcca ggcggtcgcg
    10681 ctcgccacgc cgaggcggaa gtccggcgga acgcgcacct gcgcgacctc ttcgccgagc
    10741 gcgtcgaagg cgctcagcgt ggcgtagggc tcgtactcgt cgggggtgat gtcgaggcgg
    10801 acgtcccact gcgcgtcgag cgcgaacacc gtgaggcgct tgtgcagcat cgcgtgcgcc
    10861 tgctggcggt gacgctgcag gacctcgccg gcggtcttga ccagggtgtt gaaggcggac
    10921 tggtcgagcg gtttggggtt cttcttgtcg cgccccatgg tccatgggcc caccagcgcc
    10981 ggctcggcct cgccgtcctg cagcatctcg accgcccagc cgtcgtcgtc ctcgttcttg
    11041 atcacgcgcg cggtccagcc gtcgagctgc cacagacggt cgctgcggag ggcgggcacg
    11101 ccgggctggg gcggagtgaa gagggcctgg gcttcgtccc gggcggggtc ggggaggctg
    11161 gggttcgtca tgagggccgt gggcgtggta tcggtatggg gcgcatcgag cgggagctcg
    11221 agcgcgatgg agacggtgag ggatggcgcg tcggacggca ttggcatgac cgcgtccgta
    11281 tgcggagccg cccgtggctt gcgcgttcgg cgctgcggaa gcgatggaga cgccgacgac
    11341 gcctgtgctg caaacaggga gagttgatcg gtgttcatgc ttgcctttct ccgaagctga
    11401 tattttatac agtcttcagg cgtgagcaag caccgtcgtc cagcgcacgc agaagtgcga
    11461 ctggatgctc cgagtcgggc cgtctatgct gaataaaagc gccgatgctc cgtggcaagg
    11521 gcgtcgcgcg tgttgcggct ttctcatcgc atgcattccg acaggagtct tcctcatgcc
    11581 caagaccgtt cccaagcaaa gcgattcgca agtccccgtc gccgcagcac ccgccggggc
    11641 cccgatcggc atcggcgaag gcttgttctc gcccaatgcc ggcgcggacg gcaagacgca
    11701 cttccgtgcc agcatcgatc tggcgacgga tggtcatccg gccagcctgc tcatcgataa
    11761 ccccggcgaa acggggacgg tgcaaggcgg ggggacttcg gtctgtctca cccatccact
    11821 gaaggtcaag ttcgaacatc tcaaggacta tctcggcacc cagggcgcga cggttcccga
    11881 atccttgtcc aatgtgatgg agagggctga actcgactgc gcggccttct attaccgcga
    11941 tagcggaccg atgctgctga gcattgcctt gaagtgttcc gatgagcaga aggggggcct
    12001 catcgcggcg ctgaccaata gtcctgaatt gggtaagctg ttcgacgtcg agggcgtgtc
    12061 cttgcgggtg ttgaaatgcc cggaggcttg ccaggaggaa ctggagacct actggaaggc
    12121 cgtgtccgcg ttgccgtcct ctccttccga agagaaggac cccaaggacg tgaatgcgcc
    12181 ccccgcgccg accgccgagg aaggggggga gcagggagcg ggcgcagccg acacgccggc
    12241 agccgtcgga tgagggcgat ccatccgcct tgccgcggag ccgcggggtg agcacgacgg
    12301 aggatgccgg gccggccacg ccgacgacgg tcgagctcga actcgaactc agggtaggcg
    12361 gccgtccact tgcgttgcgc gggacgctca ccggtggcgg gctgacaggc ctcgcgggga
    12421 cactgcgcgg cgagaagacc gacaccctgg gcggcctgct cgccggtctc ggcgaggcct
    12481 tcgccggccc cggcgcggcg ctcgatgccc tgggcggggg ggccttggcg gcgatccatt
    12541 tcgaggcggt cgagctcgcc tatcgcagta ccgcaacgcc ctgcgtcgtg ctgaacacca
    12601 ccctgggcgt cggggtcggc cacacccgga tcgtcgtcgc gaagctcctt cccgtccccg
    12661 ggcgtgcgga ggatgaaggc ggcctggtgg cgggtttcga gctcgagctc gaaccgcggc
    12721 cgatcggtga caagggcctc tcggagctga tcggcgggat cgtggtagag cgcctcggcg
    12781 tcttccatgc gagccgggac tgcagcgggc tgcacctcga gccggcgacc gcgccgggcg
    12841 ccttcgcgcc cctcgtcgag cgcgccgggg agggccgcag cctgcacgcg gggctgaacc
    12901 tgtcggggcg agtgctcatc ggtggcgtcg atctgctcga aggtttcggg cacgacaccg
    12961 gcaataccct cgacgcacag gccccggccg ccgagcctgc ccccgcagcc gcccccgcac
    13021 tcgcatcgcc cgcgcccgaa gcacccctgc cggcgcgcgg caaggcgttc tggaagcgca
    13081 tcg
//
LOCUS       DAOQBS010000084        13027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3871_length_13027_cov_13.636602, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000084 DAOQBS010000000
VERSION     DAOQBS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 13027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13027
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3871_length_13027_cov_13.636602"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PKN25_10265"
     CDS             <1..965
                     /locus_tag="PKN25_10265"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNV91324.1"
                     /translation="VTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLDLPQAQKAVKG
                     VMMELEDCAFPLLAGMIATDDPNVAFKDADVCLLVGARPRGPGMERADLLTANGAIFT
                     VQGKAIAENAKEDVRVLVVGNPCNTNAYIARAAAIKVGRTNPNNYHGMLRLDHNRALS
                     QLAAKSGREVSSLKKMVVWGNHSPSMYADYRFCESNGDSVKALINDHAWNNDVFLPTV
                     GKRGAAIIEARGLSSAASAANAAIDHVHDWVLGSNDWVTMGVPSDGSYGIPEGVVFGF
                     PCECKGGQFKIIQGLEIDEYSKGKIAITLKELEDERAAVADMLK"
     gene            1113..2111
                     /locus_tag="PKN25_10270"
     CDS             1113..2111
                     /locus_tag="PKN25_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNV91325.1"
                     /translation="MSLQPAQVLFAGEKPFPALPAVDHYAGAEKLMMKALKLQQELGP
                     VFDLTCDCEDGAQAGAEAEHAQMVVEVVNGAENHFGRVGARIHDVTHPHWQRDLEILV
                     GGAGRRLAFVTIPKVRSAADAAEQIRGLRRVEAACGLERALPVHVLIETHGALREAWE
                     IAALDGVESIDFGLMDFVSGHHGAIPGNAMKSPGQFEHPLVVRAKADIAAAALANGVV
                     PSHNVTTELQDVAVIRNDAERARREFGFLRMWSIHPNQILPIIAAMQPDFSEVEEAAA
                     ILCAAQDNAWGPIRHQGRLHDRASYRYYWELLQRARNTGMELPGETRQRFFTGAGH"
     gene            complement(2092..3018)
                     /locus_tag="PKN25_10275"
     CDS             complement(2092..3018)
                     /locus_tag="PKN25_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNV91326.1"
                     /translation="MELRHLRYFIAVAEELNFTRAATRLHIGQPPLSMQIRDLEEEIG
                     VRLFERGPRRVALSTAGERFLLHARRILDGVEEAVAEARRAARGELGELRVGFTSSLP
                     FTDLLPDALNAYRRRFPQVRLQLREMFTPEQFQALVSGELDVGFVRLQAGTPPAGIVL
                     REVARNPLRLVVNAAHRLAGAAQVHLAELAGEDFISFPADVGTDLPGVLRALCRQAGF
                     EPRIVQTAREATTQIGLVAAGLGVAVLPAPLESVRIARVRYLALAEPEAQFRLALAIR
                     EGEPVPTLGGFLEVLESLRAGEPPLALSVPRQ"
     gene            3213..4361
                     /locus_tag="PKN25_10280"
     CDS             3213..4361
                     /locus_tag="PKN25_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV91327.1"
                     /translation="MFVGGFATFAMVYSTQPLLPLLAAEFGVGAASASLTVSATTAGL
                     ALMLIPASVLADRLGRQQVMKAALVIAALFALATAFAPDFGSLLVLRTLLGGVIAGLP
                     AAAMAYIGEEIAPGAQARAMGLYIAGNALGGMSGRFVAALLTDWSSWRVALGMIGVLG
                     AVSALVFWRRLPASRNFRARAATPARIFADTLAIYRDPGLPALFLVAFLAMGGFVGLY
                     NFLGFRLLEPPYGLGQSAIGAIFLLYLVGTWASAASGRLAERRGRRKVLWPMVAVMGA
                     GLALTLARPLWLIIAGVGVFTFGFFAAHALASGWVGRRAGERRALASALYLSSYYLGA
                     SVLGSLAGTAWADWRWAGVAALVGLCVLFTGACALWLRRLPAPAGGTD"
     gene            complement(4379..5287)
                     /locus_tag="PKN25_10285"
     CDS             complement(4379..5287)
                     /locus_tag="PKN25_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV91328.1"
                     /translation="MKRAQANGLLLVAALIWGTAFVAQQTGMRDVGPFTFTGARFLFG
                     AVMVAPLAVLELRRLGRRGTFLDARDLLACALLGAVLFLGAALQQIGIMSTTVSNAGF
                     LTALYVPLVPIAGALLLRERSHWSVWPASLGCVAGTYLLGGGSLAALSVGDLWVIASA
                     LFWAAHVMLVGRVAARKGASVTVAMLQFLVVGGLGMAVGFGTEEVSSGALERALPSIA
                     YAGILSVGVGFTLQVVAQRHTPAADAAIVLSCETLFAALAGRFVLGETLTAEQMAGGL
                     LIFACVLAVQILPLWRERRTVSVEAG"
     gene            5520..6452
                     /gene="rocF"
                     /locus_tag="PKN25_10290"
     CDS             5520..6452
                     /gene="rocF"
                     /locus_tag="PKN25_10290"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNV91329.1"
                     /translation="MENLKVSLIGVPTDVGAGVRGASMGPEALRVAGLGRAIAAFGAE
                     VRDCGNLAGPSNPELPAVNGFRHLAEVAQWNLAVHQAVYAELSEGRVPIMLGGDHCLA
                     IGSISAVARHCRETGRKLRVLWFDAHADLNTATMTPSGNIHGMPVACLCGHGPEMLTG
                     IGGEMPAIRPEQIRQIGIRSVDEGEKRFLHDLCVEVFDMRYIDEIGMRATMEQALAGV
                     DQDTHLHVSLDVDFLDPTIAPGVGTTVRGGPTYREAELCMEMIADTGQLASLDIVELN
                     PALDHCNMTAELAVDLVESLFGKSTLMRMHRNYR"
     gene            complement(6571..7353)
                     /locus_tag="PKN25_10295"
     CDS             complement(6571..7353)
                     /locus_tag="PKN25_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV91330.1"
                     /translation="MNHKASVEQVRVEDLHKSYGDHEVLKGVSLTANSGDVISIIGSS
                     GSGKSTWLRCINFLECPTAGRILVGGKEVELVRDRKGELVVKDAHQLQQIRTRLSMVF
                     QHFNLWSHMTVLENVVEAPVHVLGLSKVAARERAERYLERVGVWKFRDAYPAHMSGGQ
                     QQRVAIARALAMEPAVLLFDEPTSALDPELVGEVLKVMRSLAEEGRTMMVVTHEMGFA
                     REVSNHVIFVHQGRVEEEGDPKEVLVRPRSERLQQFLSGNLK"
     gene            complement(7466..8200)
                     /locus_tag="PKN25_10300"
     CDS             complement(7466..8200)
                     /locus_tag="PKN25_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV91331.1"
                     /translation="MEFFDFKVITGSLPEFWRGLWMTVQLTGLALLFGFFVAIPLAVM
                     RLSPNRLVSAPVAAYTYFFRGTPMLVQLLLVYYGASQWEWMRAAWEAGHPLWSLFREP
                     YFCALFAFGLNTCAYTVEILAGSMRNTPHGELEAAKAMGMTRLKALQRIVIPSALRRM
                     IPSYSNEVILMLQGSAIASAVTLVDLTGAARNVYSRHFAPFESFVFVGLIYLALTFAL
                     VGLFKLAEHRWLAHLAPRKAEAGRQS"
     gene            complement(8209..8901)
                     /locus_tag="PKN25_10305"
     CDS             complement(8209..8901)
                     /locus_tag="PKN25_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV91332.1"
                     /translation="MDALIEYSPSLLAGAWVTLKVALMSVLLALATGLIGAACKLSGF
                     LPLRLWTMLYTTVMRGVPDLVMMLLVFYGGQLLLNAVIDYFGWDFVEIDPFVAGVLTI
                     GLIFGAYFTETFRGAFLAVSAGQLEAGRAYGMSAWQVFRRILFPQMLRFALPGIGNNW
                     LVLLKSTAIVSMIGLSDMTRIADQAGRSTHQPFTFYLAVCVLYLAMTALSSYGLGVLT
                     RRYSAGVRETRV"
     gene            complement(9008..9799)
                     /locus_tag="PKN25_10310"
     CDS             complement(9008..9799)
                     /locus_tag="PKN25_10310"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV91333.1"
                     /translation="MKKYALLCAVLALHAGAGFAKDWNEIRLASEGAYPPFNLIAADG
                     SLQGFDIDIGNALCEEMKAKCTWVKQEWDGMIPALVSRKFDAIIASMSITDERKAKVD
                     FTEKYYASPLALIAKKGSTLRPDLPSLAGKKVGVQRGTVSDNFATKYWDGKGMQIIRY
                     AKQDEAYLDLRAGRLDAAFSDYLEAYGGFLTKPEGAGYDVAGERLFGKDADEKAVIGE
                     GIGIAVRKRDKELTEKLNQALGAIRANGKYDAIQKKYFPMDIYGN"
     gene            10103..11119
                     /locus_tag="PKN25_10315"
     CDS             10103..11119
                     /locus_tag="PKN25_10315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNV91334.1"
                     /translation="MPSSAPLSVLDLAPVAAGSTPADALRNTRELAQLADRCGYTRYW
                     LAEHHNMTGIASAATAVVIGHIAGATRRIRVGAGGIMLPNHAPLVIAEQFGTLESLFP
                     GRIDLGLGRAPGTDQTTARALRRNLAGSDDQFPHDVLELQSYFQPASPGQAVRAVPGE
                     GLQVPIWLLGSSLYGAELAAHLGLPFAFASHFAPRFLFQAVEIYRSNFRPSAQLDKPW
                     FAVAANAIAADTDADAERLLRSLRLRFAAMARGVRGQLQAPEAFIEADWSPAELAFAE
                     QSLACSAVGAPDTVRQQLEALIERTGANELILTAQIFDHGARLRSMELVAQAWGLAVE
                     EAAA"
     gene            complement(11256..>13027)
                     /gene="lon"
                     /locus_tag="PKN25_10320"
     CDS             complement(11256..>13027)
                     /gene="lon"
                     /locus_tag="PKN25_10320"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNV91335.1"
                     /translation="RVKRQMEKSQREYYLNEQVKAIQKELGEGEEGADLEEMDKKIKA
                     AGMPKEALAKAEAEFKKLRLMSPMSAEATVVRNYIDTLISLPWKKKSRVSKDLGEAEK
                     VLDKDHYGLERVKERILEYLAVQQRVDKVKAPILCLVGPPGVGKTSLGQSIAKATNRK
                     FIRMALGGVRDEAEIRGHRRTYIGSMPGKILQNMSKVGVKNPLFLLDEVDKLGMDFRG
                     DPSSALLEVLDPEQNHTFQDHYVEVDFDLSDVMFVATANTLNIPAALLDRMEVIRLSG
                     YTEDEKINIAQRYLLPKQMKNNGLKREELSVTEEAVRDIVRYYTREAGVRSLEREISK
                     VCRKVVKQLVLKSRTSKVVVNARNLDKFLGVRKYSFGMAEKENQIGQVTGLAWTEVGG
                     ELLTIEAVQLPGKGKVVTTGKLGEVMQESIQAALSVVRRRARSLGIDPDFYQKSDLHI
                     HLPEGAIPKDGPSAGIAISTALVSVLTGIPVRCDVAMTGEITLRGEVLAIGGLKEKLL
                     AAVRGGIRTALIPEENVKDLAEIPDNIKNAIEIVPVKWIDRVLELALERKPEPLPEEP
                     APAAPATPAAEGVEGDQANLRPH"
BASE COUNT         2082 a         4635 c         4287 g         2023 t
ORIGIN      
        1 ctgtcaccgg cgccgccggc cagatcggct acagcctgct gttccgcatc gccagcggcg
       61 aaatgctggg caaggaccag cccgtcatcc tgcagctgct cgacctgccc caggcccaga
      121 aggccgtcaa gggcgtgatg atggagctgg aagactgcgc cttcccgctg ctcgccggca
      181 tgatcgccac cgacgatccc aacgtcgcat tcaaggacgc cgacgtgtgc ctgctggtcg
      241 gcgcccgtcc gcgcggcccc ggcatggagc gtgccgacct gctgaccgcc aacggcgcca
      301 tcttcaccgt ccagggcaag gccatcgccg agaacgccaa ggaagacgtg cgtgtgctgg
      361 tcgtgggcaa cccctgcaac accaacgcct acattgcacg cgccgccgcc atcaaggtcg
      421 gtcgcaccaa cccgaacaac taccacggca tgctgcgcct ggaccacaac cgcgcgctgt
      481 cgcagctcgc cgccaagtcc ggccgtgaag tgtcctcgct caagaagatg gtcgtgtggg
      541 gcaaccactc gccgtcgatg tacgccgact accgcttctg cgagtcgaac ggcgacagcg
      601 tcaaggcgct gatcaacgac cacgcctgga acaacgacgt gttcctgccc accgtcggca
      661 agcgcggcgc cgccatcatc gaggcccgcg gcctgtcgtc ggctgcttcg gccgccaacg
      721 ccgccatcga ccacgtgcac gactgggtac tcggctcgaa cgactgggtc accatgggcg
      781 tcccgtccga cggctcctac ggcatccccg agggcgtcgt gttcggcttc ccctgcgagt
      841 gcaagggcgg ccagttcaag atcatccagg gtctggagat cgacgagtac tccaagggga
      901 agatcgccat caccctgaag gaactcgagg acgagcgcgc ggccgtggcc gacatgctca
      961 agtaagacgc gattgcgggc ggccgcgtgc cgccctcgcg acaggccacg gggtagaatc
     1021 cgtggccttt ttcatttccg cgccgcgccg gagccccgcg cactgcccgc cctgcggccg
     1081 ccgaaccgca ccccccaacc gaggagcccg aaatgtcgct tcaacccgcc caagtcctgt
     1141 tcgccggcga gaagcccttc cctgccctgc ccgccgtcga ccattacgcc ggcgccgaga
     1201 agctcatgat gaaggcgctc aagctgcagc aggagctcgg cccggtgttc gacctcacct
     1261 gcgactgcga ggacggcgcc caggctggcg ccgaggccga gcatgcgcag atggtggtcg
     1321 aggtggtcaa tggcgcggag aaccacttcg gccgcgtggg cgcccgcatc cacgacgtca
     1381 cccatccgca ctggcagcgt gacctcgaga tcctcgtcgg cggcgccggc cggaggctgg
     1441 ccttcgtgac gatccccaag gtgcgctcgg ccgcggatgc cgcggaacag atccgcgggc
     1501 tgcgccgcgt cgaggccgcc tgcggcctcg agcgcgccct gcccgtacac gtgctcatcg
     1561 agacccacgg cgcgctgcgc gaggcctggg agatcgccgc gctcgacggc gtggagtcga
     1621 tcgacttcgg cctcatggac ttcgtctccg gccaccacgg cgccattccc ggcaacgcca
     1681 tgaagagccc cggccagttc gagcacccgc tggtggtgcg cgccaaggcc gacatcgccg
     1741 ccgccgcgct cgccaacggc gtggtgccct cgcacaacgt caccaccgag ttgcaggacg
     1801 tcgccgtgat ccgcaacgac gccgagcgcg cccggcgcga gttcggcttc ctgcgcatgt
     1861 ggagcatcca ccccaaccag atcctgccga tcatcgcggc gatgcagccc gacttctccg
     1921 aggtcgagga agccgcggcg atcctgtgcg ccgcgcagga caatgcctgg ggcccgatcc
     1981 gccaccaggg gcgcctgcac gaccgcgcgt cctaccgcta ctactgggag ctgctgcagc
     2041 gcgcgcgcaa caccggcatg gagttgcccg gcgagacccg ccagcgcttc ttcactggcg
     2101 cgggacactg agcgcgagag gcggctcgcc cgcgcgcagg ctctccagca cctccaggaa
     2161 gccgccaagc gtgggcaccg gctcgccctc ccggatcgcc agcgcaagac ggaactgcgc
     2221 ctccggctcg gccagcgcca ggtagcgcac gcgcgcgatc cgcaccgact cgagcggcgc
     2281 cggcaacacc gccacgccca gcccggcggc caccaggccg atctgggtcg tggcctcgcg
     2341 cgcggtctgc acgatgcgcg gttcgaatcc cgcctgccgg cacagcgcac gcagcacgcc
     2401 gggcaagtct gtgccgacgt cggccgggaa gctgatgaag tcctctccgg cgagttcggc
     2461 aaggtgcact tgcgcagcgc ccgccagccg gtgggcggca ttcacgacca ggcgcagcgg
     2521 attgcgcgcg acctcgcgca gcacgatccc cgccggcggc gtccctgcct gcaggcgcac
     2581 gaagccgacg tcaagctctc cgcttaccag ggcctggaac tgctccggcg tgaacatctc
     2641 ccgcaactgc aggcgcacct gcgggaagcg ccggcggtag gcgttgagtg catcggggag
     2701 gaggtccgtg aagggcagcg aggaggtgaa gccgacgcgc agctcgccca gctccccgcg
     2761 cgcggcacgg cgggcttcgg ccacggcctc ttccaccccg tcgaggatgc ggcgcgcgtg
     2821 cagcaggaag cgctcccctg cagtgctcag cgcaaccctg cgcgggccgc gctcgaacag
     2881 gcgcacgccg atctcctctt ccaggtcgcg gatctgcatc gacagcggcg gctggccgat
     2941 gtgcaggcgt gtcgcggcgc gggtgaagtt gagttcctcg gcgacggcga tgaaatagcg
     3001 caggtggcgc agctccatca ttcatttcca tatatggatc aagtgctcag aatatattgg
     3061 acgatgggtt cggcgccatc ctaagatcgc tgcagatccg ctcggcctat cgccaccgct
     3121 acgccatgcc cgcacactcc gccgctcgtc ccggcccctc gcccgaccag ctcgaggccg
     3181 gtacgcccgg ctaccgccgc gccaacctgg cgatgttcgt cggcggcttc gcgaccttcg
     3241 ccatggtcta tagcacccag cccctgctgc ccctgctcgc tgccgaattc ggtgtgggtg
     3301 cggcgagtgc cagcctgacg gtgtcggcca ccactgccgg actcgcgctg atgctgattc
     3361 cggccagcgt gctcgccgac cgcctcggcc gccagcaggt catgaaggcg gcgcttgtga
     3421 tcgccgccct gttcgcgctg gccaccgcct ttgcgcccga cttcggctcg ctgctggtct
     3481 tgcgcacact cctcggcggg gtcatcgccg ggctccccgc cgccgccatg gcctacatcg
     3541 gcgaggagat cgcccccggc gcgcaggcac gcgcgatggg gctctacatt gccggcaacg
     3601 ccctcggcgg catgagcgga cgcttcgtcg ccgcgctgct gaccgactgg agctcctggc
     3661 gggtcgcgct cgggatgatc ggcgtgctgg gggcggtctc cgcactggtg ttctggcgcc
     3721 gcctgccggc ctcgcgcaac ttccgcgcac gcgccgccac gccggcgcgg atcttcgcgg
     3781 acactctggc gatctaccgc gatcccggcc tgcccgcgct cttcctggtc gccttcctcg
     3841 ccatgggcgg cttcgtcggg ctgtacaact ttctcggctt ccgcctgctc gagccgccct
     3901 acgggctggg ccagagcgcg atcggcgcga tcttcctgct ctacctggtc ggcacctggg
     3961 cctcggcggc gagcggccgc ctcgccgagc gccgcggccg gcgcaaggtg ctgtggccga
     4021 tggtcgcggt gatgggcgcc gggctcgcgc tcaccctggc gcggccgctg tggctgatca
     4081 tcgccggcgt cggcgtgttc accttcggct tcttcgcggc gcacgccctc gccagcggct
     4141 gggtgggccg gcgcgccggc gaacggcgcg cactcgcctc ggccctttat ctgtcgagct
     4201 actacctcgg cgccagcgtg ctcggcagcc ttgccggcac ggcctgggcc gattggcgat
     4261 gggcgggcgt ggccgcgctg gtggggctgt gcgtgctgtt caccggagcc tgcgcgctgt
     4321 ggctgcggcg actgccggcg cccgcgggcg gtacggactg aaggacgcgc aggccgcctc
     4381 agcccgcctc caccgacacg gtccgtcgtt cccgccacaa aggcaggatc tgcaccgcca
     4441 gcacgcaggc gaagatcagc aggccgccgg ccatctgctc ggcagtgagc gtctcaccca
     4501 gcacgaagcg gccggccagc gcggcgaaca gggtctcgca gctcagcacg atggcggcgt
     4561 cggccgccgg ggtgtggcgt tgcgccacca cctgcagggt gaagcccacg cccaccgaca
     4621 ggatgccggc gtaggcgatc gacggcagcg cacgctcgag cgcgcctgac gagacctcct
     4681 cggtgccgaa gcctaccgcc atgccgagcc ccccgacgac gaggaactgc agcatcgcca
     4741 ccgtcaccga ggcccccttg cgcgccgcca cgcgccccac cagcatcacg tgggcggccc
     4801 aaaacagcgc gctcgcgatc acccacaagt cgcccaccga aagcgcggcc aggctgccgc
     4861 cgccaagcag gtaggtgccc gcgacgcagc ccaggctcgc cggccacacc gaccaatgcg
     4921 agcgctcgcg cagcagcagc gcgccggcga tcggcacgag ggggacgtag agcgcggtga
     4981 ggaaaccggc attgctcacc gtggtgctca tgatgccgat ctgctgaagc gccgcgccga
     5041 ggaacagcac cgcgccgagc agcgcgcaag caagcaggtc gcgcgcgtcg aggaaggtgc
     5101 cacgacgacc gaggcgtcgc agttcgagga cggcgagcgg cgccaccatc accgcaccga
     5161 agaggaagcg cgcgccggtg aacgtgaacg gcccgacgtc gcgcatgccg gtctgttgcg
     5221 cgacgaaggc ggtgccccag atgagcgccg cgacgagcag caaaccgttg gcctgcgcgc
     5281 gcttcatctc gtcgcctcga cggcgggccg ggtgaaaaca cgggaaagac gaattgaaac
     5341 agtcgacacg gatactccca agggcacggg cgccatcgat cgagagccgg ggcaccgtcg
     5401 cgcgcgcggc agatcacccg gcccggaaaa aaaggcgcaa gtgttacacg ctcgggggcg
     5461 cccccgtcaa tcccgaccgc agctgccatc gcggcaacac ctgaggtaat ctcgcggcca
     5521 tggaaaatct caaagtcagt ctgatcgggg tgcccaccga cgtcggcgca ggcgtgcgcg
     5581 gcgcgagcat gggaccggag gccttgcgcg tcgccggcct ggggcgggcc atcgccgcct
     5641 tcggcgccga ggtgcgcgac tgcggcaacc tcgccggccc gtccaacccg gagctgccgg
     5701 cggtaaacgg cttccgccac cttgccgaag tggcgcagtg gaacctcgcg gtccatcagg
     5761 ccgtttacgc ggaactgtcc gaagggcgcg taccgatcat gctcggcggc gatcattgcc
     5821 tcgcgatcgg ctcgatcagc gcggtggccc gccactgccg cgagaccggc cgcaagctgc
     5881 gcgtgctgtg gttcgacgcc catgccgacc tcaacaccgc caccatgacg ccgtcgggca
     5941 acatccatgg catgccggtc gcctgcctgt gcggccatgg ccccgagatg ctgaccggca
     6001 ttggcggcga gatgccggcg atccggcccg agcagatccg ccagatcggc atccgcagcg
     6061 tggacgaggg cgagaagcgc tttctgcacg atctctgcgt cgaagtcttc gacatgcgct
     6121 acatcgatga gatcggcatg cgcgcgacca tggagcaggc gctcgccggc gtggaccaag
     6181 acacgcacct gcacgtgagc ctggacgtgg acttcctcga cccgacgatc gccccgggcg
     6241 tcggcaccac ggtgcgcggc ggaccgacct accgcgaggc cgagctgtgc atggagatga
     6301 tcgccgacac cggccagctc gcctcgctcg acatcgtgga gctcaacccg gcgctcgacc
     6361 actgcaacat gaccgccgag ctggcggtcg atctggtcga gagcctgttc ggcaagagca
     6421 cgctgatgcg catgcaccgc aactaccgct gaacgcagcg ggacacccgc cgactcggct
     6481 tgtcacggtg cttgtgcgcg acggccttca agggcgtcgg ccggagagcg tgggccgccg
     6541 tgccgtaaca cgatcggcgc accggtcaga tcacttcaga ttgcccgaga ggaactgctg
     6601 caggcgttcg ctgcgcgggc gcaccagcac ctccttgggg tcgccctcct cctcgacgcg
     6661 cccctggtgc acgaagatca cgtggttgga cacctcgcgc gcgaagccca tctcgtgcgt
     6721 caccaccatc atcgtgcggc cttcctcggc gagcgagcgc atcaccttga gcacctcgcc
     6781 gacgagttcg gggtcgagcg ccgacgtcgg ctcgtcgaac agcagcaccg caggctccat
     6841 cgccagcgcg cgcgcgatcg ccacgcgctg ctgctggccg cccgacatgt gcgcaggata
     6901 ggcatcgcgg aacttccaca cccccacccg ctccaggtag cgctcggcgc gctcgcgggc
     6961 cgcgaccttc gacagtccga gcacatgcac cggcgcctcg acgacgttct cgagcaccgt
     7021 catgtggctc cacagattga agtgctggaa caccatcgac aagcgcgtgc ggatctgctg
     7081 caactggtgc gcgtccttga ccaccagctc gcccttgcgg tcacgcacca gctcgacctc
     7141 cttgccgccc accaggatgc gcccggcggt cgggcactcg aggaagttga tgcagcgcag
     7201 ccaggtgctc ttgccggagc cggaggaccc gatgatgctg atcacgtcgc ccgagttcgc
     7261 ggtcagcgag acgcccttga gcacctcatg gtcgccgtag gatttgtgca agtcttcgac
     7321 gcggacctgt tcgacgctgg ccttgtggtt catgttgttc tcttgcatcg gggcttgcgc
     7381 ggcacccggc atgtcgccac gagccgcggc gaaccgccgc caggaatcgg cagcgttcgc
     7441 cgcttcgggc tcgcggcagg gttgatcagg actgcctgcc cgcctccgcc ttgcgcggag
     7501 ccaggtgggc gagccagcgg tgctcggcga gcttgaacag ccccaccagg gcgaaggtga
     7561 gcgcgaggta gatcaggccg acgaagacga aggactcgaa gggtgcgaag tggcgcgaat
     7621 acacgttgcg cgccgcaccg gtgaggtcca ccagggttac cgcgctggcg atcgccgacc
     7681 cctgcagcat caggatcacc tcgttgctgt agctcgggat catgcgccgt agcgccgacg
     7741 ggatcacgat gcgctgcagg gccttcaagc gcgtcatgcc catcgccttg gcggcctcga
     7801 gctccccgtg cggcgtgttg cgcatgctgc cggcgaggat ctcgacggtg taggcacagg
     7861 tgttgaggcc gaaggcgaac agcgcgcaga agtagggttc gcggaacagc gaccacagcg
     7921 gatgcccggc ctcccacgcc gcgcgcatcc attcccactg cgaagcgccg tagtacacca
     7981 gcagcaactg cacgagcatc ggcgtgccgc ggaagaagta ggtgtaggcc gcgacagggg
     8041 ccgacaccag acggttgggc gacagccgca tgaccgcgag cgggatcgcc acgaagaagc
     8101 cgaacagcaa cgccaagccg gtgagctgga cggtcatcca cagcccgcgc cagaactcgg
     8161 gcaagctgcc ggtgatgacc ttgaagtcga agaattccat gtccgccctc agacccgggt
     8221 ctcgcgcacg ccggcactgt agcgccgtgt gagcacgccg aggccgtagc tcgacagcgc
     8281 ggtcatcgcc aggtagagga cgcagaccgc aagatagaag gtgaatggct ggtgcgtcga
     8341 acgcccggcc tggtcggcga tccgcgtcat gtcggagagg ccgatcatgg acacgatcgc
     8401 cgtgctcttg agcagcacca gccagttgtt gccgatgccc ggcagcgcga agcgcagcat
     8461 ctgcgggaac aggatgcggc ggaacacctg ccaggcgctc atgccgtagg cgcgcccggc
     8521 ttcgagttgg cccgccgaga cggccaggaa ggcgccacgg aaggtctcgg tgaaataggc
     8581 gccgaagatc agtccgatcg tcagcacgcc ggcaacgaag gggtcgatct cgacgaagtc
     8641 ccagccgaag tagtcgatca ccgcattgag cagcaactgc ccgccgtaga acacgaggag
     8701 catcatcacc agatcgggca cgccgcgcat caccgtcgtg tacagcatcg tccacagccg
     8761 cagcggcagg aaacccgaca gtttgcaggc agccccgatc aggccggtcg ccagcgcgag
     8821 cagcaccgac atcaaggcca ccttcagggt gacccaggcg ccggcgagca ggctggggga
     8881 gtattcgatc agggcatcca tggcgcaccg attcgcaaag gtaaacgcgc acgctcccgg
     8941 tccagggagc gtgcgcgcgg cagggtaacc gcaggggccg cgctggcttg accgcagccc
     9001 ggcatggtca gttgccgtag atgtccatcg ggaagtactt cttctggatc gcatcgtact
     9061 tgccgttggc gcggatcgcg ccgagtgcct ggttcagctt ctcggtgagc tccttgtcgc
     9121 gcttgcgcac cgcgatgccg atgccctcgc cgatcaccgc cttctcgtcg gcgtccttgc
     9181 cgaagaggcg ttcgccggcg acgtcgtagc ccgcgccctc gggtttggtc aggaaaccgc
     9241 cgtaggcctc gaggtaatcg gagaaggccg catcgagacg tccggcgcgc aggtcgaggt
     9301 aggcctcgtc ctgcttggcg tagcggatga tctgcatgcc cttgccgtcc caatacttgg
     9361 tggcgaagtt gtccgatacc gtgccgcgct gcacgccgac cttcttcccg gccagggagg
     9421 ggagatcggg ccgcagggtc gagcccttct tcgcgatcag ggccagcggc gacgcatagt
     9481 acttctcggt gaaatccacc ttggccttgc gctcgtcggt gatcgacatc gaggcgatga
     9541 tcgcatcgaa cttgcgcgag accagcgccg ggatcatgcc gtcccactcc tgcttcaccc
     9601 aggtgcactt ggccttcatc tcctcgcaca gggcgttgcc gatgtcgata tcgaaacctt
     9661 gcaggcttcc gtcggcggcg atgaggttga agggagggta ggcgccctcg gacgccaggc
     9721 ggatctcgtt ccagtccttg gcaaaaccgg caccggcgtg aagggcgagc accgcgcaca
     9781 gaagggcgta ttttttcatc cagaactcct cgattgtctt gttgtcggtt cttccgcgcc
     9841 gctgcggcgg agcctcacgc acagcgacag atcgaccggc agtgcagagg gggcgaatgg
     9901 tgtcaaaaaa cgccctccac ctcaattgcc gtattccacg atctgtgccc gttccggggc
     9961 cctcggggcg ccacgatcga caccgcgagg aagtttccgg caagcgtatc acgccacgca
    10021 atccgactgg cgctcgcccc cgggctggca ccggcggcgg ctctatggcg taatggcgga
    10081 cctcgttcca accgactttt ccatgccctc ctccgccccc ctctcggtgc tcgacctcgc
    10141 accggtcgcc gccggcagca cgccggccga tgcgctgcgc aacacgcgcg aactcgccca
    10201 gctcgccgac cgctgcggct acacccgcta ctggctggcg gaacaccaca acatgaccgg
    10261 tatcgccagc gccgccaccg cggtggtgat cggccacatc gccggcgcca cccggcgcat
    10321 ccgcgtcggt gccggcggca tcatgctgcc caaccacgca ccgctggtta tcgccgaaca
    10381 gttcggcacc ctggagtcgc tctttccggg ccgcatcgac ctcggtctcg ggcgtgcgcc
    10441 gggcaccgac cagaccacgg cgcgcgcgct gcgccgcaat ctcgccggca gcgacgacca
    10501 gttcccgcac gacgtgctcg agctgcagtc gtacttccag cccgccagcc ccggacaggc
    10561 cgtgcgcgcg gtgcccggcg aagggctgca ggtgccgatc tggctgctcg gctccagcct
    10621 ctacggtgcc gagctcgccg cccacctcgg cctgcctttc gccttcgcct cgcacttcgc
    10681 accgcgcttc ctgttccagg cggtcgagat ctatcggagc aatttccgtc cgtccgccca
    10741 gctcgacaag ccttggttcg cagtcgccgc caacgcgatc gccgccgaca ccgacgccga
    10801 tgcggagcgc ctcttgcgct cgctacggct gcgcttcgcg gcgatggcgc gcggcgtgcg
    10861 cgggcagttg caggcgccgg aagccttcat cgaggcggac tggtcaccgg ccgagctcgc
    10921 cttcgccgag cagtcgctcg cgtgctcggc ggtgggtgcg cccgacacgg ttcgccagca
    10981 gctcgaggcg ctgatcgagc gtaccggcgc gaacgaactc atcctcaccg cacagatctt
    11041 cgatcatggc gcgaggctgc gctcgatgga actcgtggca caggcctggg gcttggcggt
    11101 ggaggaggcc gcggcctgag cgcacacctg ctgggcggcc accgacggag gtcgaaggcc
    11161 gtgcaggtgc gacgcaaagc aataaagccg cgggcccttg cggggcgcca cggcttattg
    11221 gatccggcac cggcccgagg gccgggacgg caggtttagt gcggacgcag gttggcctgg
    11281 tcgccttcga caccttcggc cgccggcgtg gcgggcgccg ccggggccgg ctcctcgggc
    11341 agcggctcgg gcttgcgctc gagggcgagt tcgagcacgc ggtcgatcca cttcaccggc
    11401 acgatctcga tcgcgttctt gatgttgtcg gggatctcgg cgagatcctt gacgttctcc
    11461 tccggaatca gcgcggtgcg gatgccacca cggacggcgg cgagcagctt ctccttgagg
    11521 ccgccgattg ccagcacctc gccgcgtagc gtgatctcgc cggtcatcgc cacgtcgcaa
    11581 cgcaccggga tgccggtgag caccgacacc agcgcggtcg aaatcgcgat acccgccgac
    11641 ggaccgtcct tggggatcgc gccctccggc aggtggatgt gcagatcgct cttctggtag
    11701 aagtccgggt cgataccgag cgaacgcgca cgccggcgca ccaccgacag cgcggcctgg
    11761 atggactcct gcatcacctc gccgagcttg ccggtggtca cgaccttgcc cttgccgggg
    11821 agctgcaccg cctcgatggt cagcagttcg ccacccacct cggtccatgc gagaccggta
    11881 acctggccga tctggttctc cttctccgcc atgccgaagc tgtacttgcg cacgccgagg
    11941 aacttgtcga ggttacgggc gttgaccacc accttgctcg tgcggctctt gagcaccagc
    12001 tgcttgacca ccttgcggca gaccttggag atctcgcgct ccaggctgcg cacgccggcc
    12061 tcgcgggtgt agtaacgcac gatgtcgcgc accgcctcct cggtgaccga gagctcctcg
    12121 cgcttgagcc cgttgttctt catctgcttg ggcagaaggt aacgctgggc gatgttgatc
    12181 ttctcgtcct cggtgtaacc cgacagacgg atcacctcca tgcggtcgag cagcgcagcc
    12241 gggatgttga gggtgttggc ggtggccacg aacatcacgt ccgacaggtc gaaatcgacc
    12301 tcgacgtagt ggtcctggaa ggtgtggttc tgctccgggt cgagcacctc gagcagcgcg
    12361 gacgacggat cgccacggaa atccatgccg agcttgtcga cctcgtcgag caggaacagc
    12421 gggttcttga ccccgacctt gctcatgttc tgcaggatct tgcccggcat cgagccgata
    12481 taggtgcggc ggtggccgcg gatctcggcc tcgtcgcgca cgccacccag tgccatgcgg
    12541 atgaacttcc gattggtggc cttggcgatc gactggccga gcgaggtctt gcccacgccg
    12601 ggggggccga ccaggcacag gatcggcgcc ttgaccttgt ccacacgctg ttgcacggca
    12661 aggtactcga ggatgcgttc cttgacgcgc tcgaggccgt agtggtcctt gtcgagcacc
    12721 ttctcggcct cgccgaggtc cttgctgacg cgcgacttct tcttccacgg caggctgatc
    12781 agggtgtcga tgtagttgcg caccacggtg gcctcggccg acatcggcga catcaggcgc
    12841 agcttcttga actcggcctc ggccttggcg agcgcctcct tgggcatgcc ggcggccttg
    12901 atcttcttgt ccatctcctc gagatcggca ccctcctcgc cctccccgag ctccttctgg
    12961 atggccttga cttgctcgtt gaggtagtac tcgcgctggc tcttctccat ctggcgcttg
    13021 acgcggc
//
LOCUS       DAOQBS010000085        12937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3918_length_12937_cov_17.267505, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000085 DAOQBS010000000
VERSION     DAOQBS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12937
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3918_length_12937_cov_17.267505"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(387..1265)
                     /gene="rarD"
                     /locus_tag="PKN25_10325"
     CDS             complement(387..1265)
                     /gene="rarD"
                     /locus_tag="PKN25_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNV91336.1"
                     /translation="MFAALAAFVTWGLAPLYFRALGSVPPFEIVAHRVLWSALLLALM
                     LATLRFTGGFERVGAVLRQPRLLALLALTSLLTSSNWLVFIWAIDAGRLLEASLGYFI
                     NPLVSVALGAIFLGERLRPLQAAAVGIACAGVAWRVWQLGSLPWIPLFLAGTFGLYGL
                     LRKRAPVDAVGGLFIETLVVAPLALAWLLWLHAQGSLAFGSGNPAEWLLPLTGVITAI
                     PLMLFAVGARRLPLATVGFLQYIAPSLNFLVAVLLFREPFDTTQLVGFALIWLALAVY
                     SADMVRAGRRTARRAR"
     gene            complement(1467..2336)
                     /locus_tag="PKN25_10330"
     CDS             complement(1467..2336)
                     /locus_tag="PKN25_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV91337.1"
                     /translation="MSRPTDPAQPGFDARRFKAMERAGFNRIAARYAEGAHLRAELAE
                     ALLEAAALAPGQRVLDLASGPGLLAGQAALRVAPGGSVLATDIAEGMLAEGARRCGEH
                     ADAPCFAAADAEQLCLADASMDRVLAGLALFIFPHPERALAEMHRVLMPGGRVALSVW
                     GVRESVPLIHRAQDTIARLLPPPKVSRPSVFRYGEAEVLSATLAAAGFDDIDIRPCTF
                     SCHFADAEAYWQAFLDLAGGAAEALGRLPEPIQADLRAAVADDLADHRCDDGRGGFLV
                     PALTLVASARRPG"
     gene            complement(2362..2949)
                     /locus_tag="PKN25_10335"
     CDS             complement(2362..2949)
                     /locus_tag="PKN25_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HNV91338.1"
                     /translation="MILYLHGFRSAPASIKARALKERMVERGLAERFWCAQLPVSPRA
                     AIALAETEIARIRAAGLEPTVVGSSLGGYYATWLAERHDLRAVLINPAVVAPLSLEAY
                     VGVQDNLYTGERFEFTRGHIDELRAIEVPTITRPERFWLLVETGDEVLDYRHAVDKYA
                     GARQTVLEGGDHGFSRWNDYLDEVLAYAGLGDTRP"
     gene            complement(2963..3787)
                     /locus_tag="PKN25_10340"
     CDS             complement(2963..3787)
                     /locus_tag="PKN25_10340"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNV91339.1"
                     /translation="MDLSLLLVALVLGIVEGLTEFLPVSSTGHLIILGDLLGYNDDTS
                     KVFKIVIQLAAILAVCWDYRERLIKVASGITTEPSAQRFVWLLFIGFLPAAVLGLMFH
                     STIKGLLFNPITVATALVVGGLIILYVEKRAYHPRLNSVDELRWTDALKVGFAQALAM
                     IPGTSRSGATIMGGVIFGLSRKTAAEFSFFLAIPTMFAATAYDLYKNWALLRVEDLPV
                     FAVGFIASFVAAMWAVKSFIKFISNHTFVAFAWYRIVFGIFVLATWYFGWVEWSEP"
     gene            complement(3878..4132)
                     /locus_tag="PKN25_10345"
     CDS             complement(3878..4132)
                     /locus_tag="PKN25_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV91340.1"
                     /translation="MDCRPGCAACCIAPSISSPIPGMPDGKPAGVRCVQLDEAGHCRL
                     FGDPRRPAVCASLRPGAEMCGTDREHALLFLATLEAMTAT"
     gene            complement(4136..4651)
                     /locus_tag="PKN25_10350"
     CDS             complement(4136..4651)
                     /locus_tag="PKN25_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2478 domain-containing protein"
                     /protein_id="HNV91341.1"
                     /translation="MPAHAIAAVVYKPTDNIEALLARAARALGERGVRLGGVLQHDIA
                     TVIEDPCAMQLENLETGESIPLSQELGRGSISCRVDPDALAQGSMAVRGAIERGVELV
                     IINKFGAQEVSGAGLRDEMAETVVAGVPLLTAVGERFLPEWKEFTGGEGVLLEPTLDA
                     ILAWWAAPSAG"
     gene            4765..9060
                     /gene="hrpA"
                     /locus_tag="PKN25_10355"
     CDS             4765..9060
                     /gene="hrpA"
                     /locus_tag="PKN25_10355"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HNV91342.1"
                     /translation="MRAEHKGARRPSALPNFDDCLSGDRPRLRRMARDLSHPPRADER
                     ATAQRLRLQADFDALLERSRAALRARRAALPVPDFPPELPVSGRRDEIATALAAHQVI
                     IVCGETGSGKTTQLPKIAMTLGRGVAGLIGHTQPRRLAARATASRIAQELKSPLGEVV
                     GYKIRFTDKTGERSHVKLMTDGILLAETQTDPLLAAYDTLIIDEAHERSLNIDFLLGY
                     LKTLLPRRPDLKVVVTSATLDAERFARHFADAAGKPAPVIEVSGRLYPIEMRYRPVES
                     EEIDPAAAARSAAKGGREAAKDRSRDLMDALVDAVDEAQRCGPGDVLVFLPGEREIRE
                     AAEALRKAHHLPGTEILPLFARQSAQEQARVFSASNGRRVVLSTNVAETSLTVPGIRY
                     VVDTGLARIKRYSPRNKVEQLQVEKIARSAAQQRAGRCGRVMDGICIRLYDEDDFNRR
                     PAHTDPEILRSSLAGVILRMKALKLGAVEDFPFIDAPGARLIGDGYALLAELGAVSDD
                     DERKLTPTGVELAKLPLDPRIGRMILAARDRGALAELLVIAAALSVQDPRERPQDSPG
                     AADQAHARFRGGEQDQKSEFLWFWHLWKAWDEVQRHESSSKQKAWCKKHFLNYMRMRE
                     WRDVFAQLHTLCAEHGWKENEQPANYEAIHKALLAGLLGNVGCKVEDASGPQAGAYLG
                     ARGIKFWPHPGSALAKKAGKWIMAAEQVETSRLFGRCIARIEPEWVEEVGGHLIKRQV
                     FEPHWSKASGAVRAWERGTLYGLVIYPRRGVAYRDIDPALCRELFIREGLVQGEIAEG
                     PARAMAFLAHNQRLVAEIERLEHKSRRPDVLVDETLIEAFYDSKLPAEVCDLAGLEAW
                     RKQAEKAEPKLLHLSREQLMRHDAEGVTTDRFPPTLEVLGQKLKLAYLHQPGEADDGV
                     TLAVPLAMLNQIPANRCEWLVPGLLEEKVAALMKTVPQKHRHRLQPVAESAAAFMAVF
                     EAGEFDTDEPLLKMLQRFVEERVQLKLPLESFRPENLKPHCFMNFRVIDEHGRLMGQS
                     RNLMELRARFREQVAARFSAAKIGGALAGALSVVGVGGAGGGQGVGAGGAGVSARGAG
                     SDSVAGEVAGRARSGADARDAAADVGGRAGAGQPGGKGSGKAAGDPAGRSAAPAELPA
                     QVLSGFTAWTFGALPELLEVRVAGREVIGFPALHDDGDSVSLRPHDTPEEASRVHRRG
                     LARLFALNLKDQVRAVERLPGLRELALQYMGFGTEAELKARLIEATLGRCCLLEPLPT
                     DADAFAKRCAEAKSRVSLVAQEFMRLTGQLLVEHAALQKRLSGLKTFPEVVADIQGQL
                     GALLPKDFLVAFEWDKLAHFARYLKGAAVRLDKLRNNPARDAQAMAEWKQLAQAWERE
                     RLARRRAGVEDPALEEFRWLLEELRVGLYAQELRTPMPVSVKRLQKIWESRPR"
     gene            complement(9140..9472)
                     /locus_tag="PKN25_10360"
     CDS             complement(9140..9472)
                     /locus_tag="PKN25_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91343.1"
                     /translation="MNTTLTRKIATALLCGSVLAAAAPAAYADRDDRGPRWGHHKHHY
                     KHDHRRHWDERTVYREKVIIRERPRYYREAEIHNHYYERPYRSYSYSRSPAVVIGVDI
                     PPLVIPLR"
     gene            9711..10097
                     /gene="rpsF"
                     /locus_tag="PKN25_10365"
     CDS             9711..10097
                     /gene="rpsF"
                     /locus_tag="PKN25_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNV91344.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKSIVTTRNGQIHRLEDWGR
                     RQMAYPIQKVHKAHYVLMNIECDGETLAELEHSFKFNDAVLRHLTIKMKKAVTTPSPM
                     MKEEKSRSMNIGADDAKPADSEAAAA"
     gene            10141..10413
                     /gene="priB"
                     /locus_tag="PKN25_10370"
     CDS             10141..10413
                     /gene="priB"
                     /locus_tag="PKN25_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HNV91345.1"
                     /translation="MAEVLPLRRTPAGIPVASCVLAHESKQVEAGLTREVSVEVQAVA
                     LGDLAAVLAVAVPGSALRATGFLAAKSLRSRTPVLHLNTIEFLEGN"
     gene            10416..10691
                     /gene="rpsR"
                     /locus_tag="PKN25_10375"
     CDS             10416..10691
                     /gene="rpsR"
                     /locus_tag="PKN25_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNV91346.1"
                     /translation="MAFKPKSKFKKKDDRGGRGLFKRRKFCRFTAEKIEEVDYKDVDI
                     LKDFITENAKIMPARITGTKAGYQRQLSVAVKRARFLALMPYTDLHQ"
     gene            10707..11156
                     /gene="rplI"
                     /locus_tag="PKN25_10380"
     CDS             10707..11156
                     /gene="rplI"
                     /locus_tag="PKN25_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNV91347.1"
                     /translation="MQIILLEKVVNLGGLGDVVKVKDGYARNFLIPQGKAKRATQANL
                     AEFEARRAELERVQAEKLAAAQELAGKLEGVSVEVARKAGMDGRLFGSVGNADISEAL
                     AAKGFDIERSAIRMPDGPLKQVGETQLEVSLHADVLANITVVVAAEQ"
     gene            11298..12698
                     /gene="dnaB"
                     /locus_tag="PKN25_10385"
     CDS             11298..12698
                     /gene="dnaB"
                     /locus_tag="PKN25_10385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNV91348.1"
                     /translation="MSSASSRFSDFSADPQLAQIKLPPHSLEAEQSLIGGILLDNQAW
                     ERVADLVNEADFYRDDHRRIYRHITKLIDQGRPADAVTVFESLEKNGEAEQAGGLAYI
                     AELANNTPSAANIRRYAEIVRERAILRKLVAVGDEIAASALSPSGKDAKALLDEAEAK
                     VFEIAEAGARHASGFVSIQPILKQVVDRVQELYDRDNPSEVTGVPTGLVDLDEKTSGL
                     QPSDMIIVAGRPAMGKTTFALNLAEHIAIEQRLPVAIFSMEMPGTQLATRFISSVGRI
                     DMQKIRSGRLTDDDWQRLTMAMGKLYDAPLYIDETPGLNPIDLRARARRLARQCGRLG
                     LIVIDYLQLMSGTREGDNRASELSEISRSVKSLAKELNVPIMALSQLNRSLEQRPNKR
                     PVMSDLRESGAIEQDADIIMFIYRDEVYNPDSPDKGTAELIIGKHRNGPTGTVRLTFI
                     GENTRFENYAAAPGMY"
BASE COUNT         2069 a         4280 c         4707 g         1881 t
ORIGIN      
        1 gtgcggcttc gattcggccg cggggccggg gtggcggggg ccgcctgcac tcgcggcccc
       61 tcgctgcgct cggggcttcc cgcggtggag gacgccgcgg gggcggcgac gcaaactcgt
      121 cgctgcgctc ctcggacatg cgtcgccttg tttcccccgc gacgccctcc acctcggcgc
      181 tcctgaagtt ggcccccgcc atcccctgcc ccgcggcctc gcgcattcgg ggccgatcgc
      241 gccggcagcg agccgtggca ccggggtgcg ccgtaggagc gacgcaagtc gcgatcctgg
      301 cggggatgcg accgccgacg ctgccattgg gtgccccgtc gatcggcgag gccgtcaccc
      361 gacgcggctg gcgcgcaccg cttccgtcag cgggctcgcc gcgccgtccg ccgcccggcc
      421 ctgaccatgt ccgcggaata caccgcgagc gcgagccaga tgagcgcgaa gccgacgagc
      481 tgggtggtgt cgaagggctc gcggaagagc agcaccgcga ccaggaaatt gaggctgggc
      541 gcgatgtact gcaggaagcc gaccgtggcc agcggcaggc ggcgggcgcc gaccgcgaac
      601 agcatcagcg ggatcgcggt gatcacgcca gtgagcggca gcagccattc ggccgggttg
      661 ccgctgccga aggcgaggct gccctgggcg tgcagccaca gcagccaggc gagcgcgagg
      721 ggcgcgacga cgagcgtctc gatgaagagt ccgcccacgg cgtccaccgg ggcgcgcttg
      781 cgcagcaggc cgtagaggcc gaaggtgccg gcgaggaaga gcgggatcca cggcaggctg
      841 ccgagctgcc agacccgcca ggccacgccg gcgcaggcga tgcccaccgc ggcggcctgc
      901 agcgggcgca ggcgttcgcc gaggaagatc gcgccgagcg ccacgctgac cagcgggttg
      961 atgaagtagc cgaggctggc ctcgagcagg cgcccggcgt cgatcgccca gatgaagacc
     1021 agccagttgc tgctggtgag cagcgaggtg agcgcgagca gggcgagcag gcggggctgc
     1081 cgcagcacgg cgcccacgcg ttcgaagccg ccggtgaagc gcagcgtggc cagcatcagc
     1141 gcgagcagca gcgccgacca cagcacgcgg tgggcgacga tctcgaaggg cggcacgctg
     1201 ccgagggcgc ggaagtagag cggcgccaga ccccaggtca cgaaggcggc gagggcggcg
     1261 aacatgccct ggcgcacggc gagggtgtcg ccttcgactg cggaagcggg gagtgccgcg
     1321 gtcggtgcgc gggtttggaa gcgggacaag tcgggatccg gacggggcag ggggtggaag
     1381 gaggaaggcg ggcgcgccgg gaaatcggcg gcgggccgca gtgcggcccc cggccgcgcg
     1441 ggcggcaggg ctcggcccgg ggggggtcag cccggccggc gggcgctcgc caccagggtc
     1501 agggccggca ccaggaagcc gccgcggccg tcgtcgcagc ggtgatcggc gaggtcgtcc
     1561 gcgaccgccg cgcgcaggtc cgcctggatg ggctcgggca ggcggccgag cgcctccgcg
     1621 gcaccgccgg cgaggtcgag gaaggcctgc cagtaggctt cggcgtcggc gaagtggcag
     1681 gagaaggtgc aggggcggat gtcgatgtcg tcgaagccgg ccgccgcgag cgtggcggac
     1741 agcacttccg cctcgccgta gcggaacacc gaggggcgcg acaccttggg cggcggcagc
     1801 aggcgggcga tggtgtcctg agcgcggtgg atcagcggca cggactcgcg cacgccccac
     1861 accgacagtg ccacccgtcc gccgggcatg agcacgcggt gcatttcggc gagcgcgcgc
     1921 tcggggtgcg ggaagatgaa cagcgccagc ccggcgagca cgcggtccat gctggcgtcg
     1981 gcgaggcaca gctgttcggc gtcggcggcg gcgaagcacg gagcgtcggc gtgttcgccg
     2041 cagcgacgcg cgccttcggc gagcatgccc tcggcgatgt cggtggcgag cacgctgccg
     2101 ccgggggcga cgcgcagcgc ggcctggccg gcgagcaggc cggggccgct ggcgaggtcg
     2161 agcacgcgct ggccgggggc gagcgcggcg gcctcgagca gggcttcggc gagttcggcg
     2221 cgcaggtggg cgccttcggc gtagcgcgcg gcgatgcggt tgaagccggc gcgctccatg
     2281 gccttgaagc ggcgggcatc gaagccgggt tgggcgggat cggtgggacg gctcatcggc
     2341 ggcactccgt gttcggtcgg gtcaggggcg ggtatcgccc agccccgcat aggcgagcac
     2401 ctcgtccagg tagtcgttcc agcgcgagaa gccatggtcg ccgccttcga gcacggtctg
     2461 gcgggcacca gcgtacttgt ccaccgcgtg gcggtagtcg agcacctcgt cgccggtctc
     2521 caccagcagc cagaagcgct ccggccgggt gatcgtcggc acctcgatcg cgcgcagctc
     2581 gtcgatgtgg ccgcgggtga attcgaagcg ctcgccggtg tagaggttgt cctgcacgcc
     2641 gacataggcc tcgagcgaga gcggcgcgac cacggccgga ttgatcagca ccgcgcgcag
     2701 gtcgtggcgc tcggcgagcc aggtggcgta gtagccaccg agcgagctgc cgaccacggt
     2761 gggctcgagg ccggcggcgc ggatgcgcgc gatctccgtt tcggcgagcg cgatcgcggc
     2821 gcgcggcgac accggcagct gcgcgcacca gaagcgttcg gccaggccgc gctcgaccat
     2881 gcgttccttc agggcgcgcg ccttgatcga cgccggggcg gaacggaagc cgtgcaggta
     2941 gaggatcatc gtgctgcctc gatcatggct cggaccactc gacccagccg aagtaccagg
     3001 tggcgagcac gaagatgccg aagacgatgc ggtaccaggc gaaggcgacg aaggtgtggt
     3061 tggagatgaa cttgatgaag ctcttcaccg cccacatcgc ggcgacgaag gaggcgatga
     3121 agccgaccgc gaacaccggc aggtcttcca cccgcagcag cgcccagttc ttgtacaggt
     3181 cgtaggcggt ggcggcgaac atggtcggga tcgccaggaa gaaggagaac tccgccgccg
     3241 tcttgcgcga caggccgaag atgacgccgc ccatgatggt cgcgcccgag cgcgaagtgc
     3301 ccgggatcat cgccagcgcc tgggcgaagc ccaccttgag cgcgtctgtc cagcgcagtt
     3361 catccaccga attcaggcgc gggtggtagg cgcgcttttc cacgtagagg atgatcaggc
     3421 cgccgaccac cagcgcggtg gccaccgtga tcgggttgaa cagcaggccc ttgatggtgg
     3481 agtggaacat cagcccgagc accgccgcgg gcaggaagcc gatgaacagc agccagacga
     3541 agcgctgcgc gctcggttcg gtggtgatcc cgctcgcgac cttgatcagg cgctcgcgat
     3601 agtcccagca cacggcgagg atggcggcga gctggatgac gatcttgaac accttgctgg
     3661 tgtcgtcgtt gtagccgagc aggtcgccca ggatgatgag gtggccggtg gaggacaccg
     3721 ggaggaattc ggtcaggcct tcgacgatgc cgaggacgag cgcgacgagg aggagggaga
     3781 ggtccatgcg gccgggctgg aagtggggga agggtggaag gatcggaggc cggattatag
     3841 ccggcgggat gttgcaccgc agggtaggtt tgcccgctca ggtcgccgtc atcgcttcga
     3901 gggtggcgag aaaaagcagg gcatgctcgc ggtcggtgcc gcacatctcg gcgccggggc
     3961 gcaggctggc gcacaccgcc ggccgacgcg ggtcgccgaa caggcggcag tgcccggcct
     4021 cgtcgagctg cacgcagcgc acacccgcgg gtttgccgtc cggcatgccg gggatgggcg
     4081 aggagatcga gggcgcgatg cagcaggcgg cgcagccggg gcggcagtcc atgcctcagc
     4141 cggcgctcgg cgccgcccac caggcgagga tggcgtcgag cgtgggctcg agcagcacgc
     4201 cctcgccacc ggtgaactcc ttccactcgg ggaggaagcg ctcgcctacc gccgtgagca
     4261 ggggtacgcc ggccacgacc gtctcggcca tttcgtcgcg caggccggcg cccgacactt
     4321 cctgcgcgcc gaacttgttg atgatgacca gctcgacgcc gcgctcgatc gcgccgcgca
     4381 ccgccatcga gccttgcgcg agcgcgtccg ggtcgacccg gcaggagatc gagccgcggc
     4441 cgagctcctg cgacagcggg atgctctcgc cggtctccag gttctcgagc tgcatcgcgc
     4501 aggggtcctc gatcaccgtg gcgatgtcgt gctgcagcac gccgcccagg cgcacgccac
     4561 gctcgcccag ggcgcgcgcg gcgcgggcga gcagagcctc gatgttgtcg gtgggcttgt
     4621 agacgacggc ggcgatggcg tgtgcgggca tggtcgggct cggcgggcgg ctcgttcgtg
     4681 gatgggcctc attgtcgcac tgcggcgcca acaaggcgaa gatgcgggag ataatgcgcg
     4741 gctcgacgaa gccgaaacgg atccatgagg gctgaacaca agggcgcgcg ccgcccgtcc
     4801 gcgctgccga acttcgatga ctgtctgagc ggcgaccgcc cgcgcctgcg ccgcatggcg
     4861 cgcgacctct cccacccgcc acgcgcggac gagcgcgcca cggcgcagcg cctgcgcctg
     4921 caggcggact tcgatgccct gctcgaacgc tcgcgcgccg cgctgcgcgc acgccgcgcc
     4981 gccttgccgg tgccggactt cccgcccgag ctgccagtgt ccgggcgccg cgacgagatc
     5041 gccacggccc tcgccgccca ccaggtcatc atcgtgtgcg gcgagaccgg ctcgggcaag
     5101 accacccagc tgccgaagat cgcgatgacg ctcggccgcg gcgtcgccgg cctcatcggc
     5161 cacacccagc cgcgtcgcct ggccgcacgc gccaccgcga gccgcatcgc gcaggagctc
     5221 aagagcccgc tcggcgaggt cgtgggctac aagatccgct tcaccgacaa gaccggcgag
     5281 cgcagccacg tcaagctgat gaccgacggc atcctgctcg ccgagaccca gaccgacccg
     5341 ctgctcgccg cctacgacac gctgatcatc gacgaggcgc acgagcgcag cctcaacatc
     5401 gatttcctgc tcggctacct gaagaccctg ctgccacgcc ggccggatct gaaggtggtg
     5461 gtgacctcgg cgacgctgga tgccgagcgt ttcgcccggc actttgccga tgccgcgggc
     5521 aagccggcgc cggtgatcga ggtgtcgggc cggttgtatc cgatcgagat gcgctaccgg
     5581 ccggtggaga gcgaggagat tgaccccgcc gcggccgcgc gcagcgccgc caagggtggc
     5641 agggaggccg ccaaggacag gagccgcgac ctcatggacg cgctggtcga tgcggtcgac
     5701 gaggcccagc gctgcggccc gggtgacgtg ctggttttcc tgcccggcga gcgcgagatc
     5761 cgcgaggccg ccgaggcgct gcgcaaggcg caccacctgc ccggcaccga gatcctgccg
     5821 ctgttcgcgc gccagtcggc gcaggagcaa gcgcgcgtgt tctcggcctc caacggccgc
     5881 cgggtggtgc tgtcgaccaa cgtcgccgag acctcgctga ccgtgccggg catccgctac
     5941 gtggtcgaca ccggactcgc gcgcatcaag cgctactcgc cgcgcaacaa ggtcgagcag
     6001 ctgcaggtcg agaagatcgc ccggtcggcc gcgcagcagc gcgccgggcg ctgcggtcgt
     6061 gtcatggacg gcatctgcat ccgcctctac gacgaggacg acttcaaccg ccgcccggcg
     6121 cacaccgatc ccgagatcct gcggtcgtcg ctcgccggcg tgatcctgcg catgaaggcc
     6181 ctgaagctcg gcgcggtgga ggacttcccc ttcatcgacg cgcccggcgc gcgcctgatc
     6241 ggcgacggct acgcgctgct ggccgagttg ggcgcggtca gcgacgacga tgaacgcaag
     6301 ctcaccccga ccggcgtcga gctcgccaag ctgccgctcg acccgcgcat cggccgcatg
     6361 atcctggccg cgcgcgaccg tggcgcgctc gccgagttgc tggtgatcgc cgccgcgctg
     6421 tcggtgcagg acccgcgcga gcgcccgcag gacagcccgg gtgccgccga ccaggcgcac
     6481 gccaggttcc gcggcgggga gcaggatcag aagtccgagt tcctgtggtt ctggcacctg
     6541 tggaaggcct gggacgaggt ccagcgccac gagtcctcca gcaagcagaa ggcctggtgc
     6601 aagaagcact tcctcaacta catgcgcatg cgcgagtggc gcgatgtgtt cgcccagctc
     6661 cacaccctgt gcgccgagca cggatggaag gagaacgaac agccggcgaa ctacgaggcg
     6721 atccacaagg cgctgctcgc cgggctgctc ggcaacgtcg gctgcaaggt cgaggatgcc
     6781 tcggggccgc aggccggcgc ctacctcggc gcgcgcggga tcaagttctg gccgcacccg
     6841 ggctccgcgc tggcgaagaa ggccggaaag tggatcatgg ccgccgagca ggtcgagacc
     6901 agccgcttgt tcgggcgctg catcgcgcgc atcgagccgg agtgggtgga ggaggtcggc
     6961 ggccacctga tcaagcgcca ggtgttcgag ccgcactggt cgaaggcgtc cggcgcggtg
     7021 cgcgcctggg agcgcggcac gctgtacgga ctggtgatct atccgcgccg cggcgtggcc
     7081 tatcgcgaca tcgaccccgc gctgtgccgc gagctcttca tccgcgaggg cctggtgcag
     7141 ggcgagatcg ccgaggggcc ggcgcgcgcg atggccttcc tcgcccataa ccagcgcctg
     7201 gtggcggaga tcgagcgcct cgagcacaag tcgcgccgcc ccgacgtgct ggtggacgag
     7261 accctgatcg aggccttcta cgacagcaag ctccccgcgg aggtctgcga cctggcgggg
     7321 ctggaggcgt ggcgcaagca ggcggagaag gccgagccca agctgctgca tctgtcgcgc
     7381 gagcaactga tgcgccacga cgcggaaggg gtgaccaccg accgtttccc gccgacgctg
     7441 gaggtgctcg ggcagaagtt gaagctcgcc tatctgcacc agcctggcga ggccgacgac
     7501 ggcgtgacgc tcgcggtgcc gctggcgatg ctcaaccaga tccccgccaa ccgctgcgag
     7561 tggctggtgc cgggcctgct cgaagagaag gttgcggcgc tgatgaagac cgtgccgcag
     7621 aagcaccgcc accgcctgca gccggtggcc gagagcgcgg cggccttcat ggcggtcttc
     7681 gaggccggcg agttcgacac cgacgagccg ctgctgaaga tgctgcaacg cttcgtggag
     7741 gagcgcgtgc agttgaagct gccgctggag agcttccgcc cggagaacct gaagccgcac
     7801 tgcttcatga acttccgcgt gattgacgag cacggccgct tgatggggca gtcgcgcaac
     7861 ctgatggagc tgcgtgcgcg cttccgcgag caggtggcgg cgcgcttctc ggcggcgaag
     7921 atcggcggcg cgctcgcggg ggcgctgtcg gtggtgggcg tcgggggcgc gggtggtggc
     7981 caaggcgtcg gtgccggcgg agcgggcgtc agtgcgcggg gggccggatc cgactccgtt
     8041 gcgggcgagg tcgccggccg tgcgcgctcc ggcgcggatg cgcgtgatgc cgcggcggat
     8101 gtgggtggcc gtgccggggc ggggcagccg gggggcaaag ggagcggcaa ggcggcgggc
     8161 gacccggcag gccggagcgc tgcgcctgcc gaactcccgg cgcaggtgct gtccggcttc
     8221 actgcgtgga ctttcggcgc cctgcccgag ttgctcgagg tgcgcgtcgc cggccgcgag
     8281 gtgatcggtt ttcctgcgct gcacgacgac ggcgacagcg tctcgttacg cccccacgac
     8341 acgcccgagg aggcgtcgcg cgtgcatcgc cgcggcctcg cccgcctgtt cgcgctcaac
     8401 ctcaaggatc aggtgcgcgc ggtcgagcgt ctgcccggct tgcgcgagct cgcgctgcag
     8461 tacatgggct tcggcaccga ggccgagctg aaggcgcgcc tgatcgaggc gacgctgggc
     8521 cgctgctgcc tgctcgagcc tttgcctacg gatgccgacg cgttcgcgaa gcgctgcgcc
     8581 gaggccaagt cgcgggtgag cctggttgcg caggagttca tgcgcctgac cggccagctg
     8641 ctggtcgagc atgcggcgct gcagaagcgc ctgtccgggc tgaagacctt tcccgaggtg
     8701 gtggctgata tccagggcca gctcggtgcg ctgctgccga aggacttcct cgtcgccttc
     8761 gagtgggaca agctggcgca cttcgcacgc tatctcaagg gtgccgcggt gcggctcgac
     8821 aaactccgca acaatccggc gcgcgacgcc caggccatgg cggagtggaa gcagctcgcc
     8881 caggcctggg agcgcgagcg cctcgcccgc cggcgcgcgg gcgtggaaga tccggcgctg
     8941 gaagagttcc gctggctgct ggaggagttg cgcgtgggct tgtacgcgca ggagcttagg
     9001 acgccgatgc cggtgtcggt gaagcgtctg cagaagatct gggagagccg gccgcgctga
     9061 gtcgcgcgac cggctcggcc gggcgtgccg tcaggggagt gaggccgcgc cctgcgaacg
     9121 gggatggagc ggggggtgtt cagcgcagcg ggatcaccag cggcgggata tccacgccga
     9181 tgaccacggc gggcgagcgg ctgtagctgt aagagcggta ggggcgctcg tagtagtggt
     9241 tgtggatctc cgcctcgcgg tagtagcgcg ggcgttcacg gatgatgacc ttctcgcgat
     9301 acaccgtgcg ctcgtcccag tggcggcgat gatcgtgctt gtagtggtgc ttgtggtggc
     9361 cccagcgcgg gccgcggtcg tcccggtcgg cgtaggcggc aggcgcggca gcggcgagga
     9421 cgctgccgca gagcagggcg gtggcgatct tgcgggtcag ggtcgtgttc atggtctgcc
     9481 tcgttcgatt tcttcgttgc agggcagggc cccggtgatc cggatactag gcgaggcgga
     9541 cgccacgagg cagtcgcaga gtgtaagcgg gcttttgcaa ccgcgctccc gacttccggg
     9601 cttcttcagt gtctttgatt ttccgggttt tcttgttaga ctggcgagct tcccttgctc
     9661 caccggaccg catgccgggg ggctgcaaca accaaccgca aggagtttgc atgcgacatt
     9721 atgaaatcgt cttcatcgtg cacccggacc agtccgagca ggtgccggcg atggtcgagc
     9781 gctacaagtc catcgtgacc acccgcaacg gccagatcca ccgcctggaa gactgggggc
     9841 gtcgtcagat ggcctacccg atccaaaagg tgcacaaggc ccactacgtg ctgatgaaca
     9901 tcgagtgcga cggcgagacg ctggccgaac tcgagcactc gttcaagttc aacgacgccg
     9961 tgctgcgtca cctcaccatc aagatgaaga aggccgtcac cacgccttcg ccgatgatga
    10021 aggaagagaa gtcgcgctcg atgaacatcg gtgccgacga cgccaagccg gccgacagcg
    10081 aagccgctgc cgcctgagtt gcctgagccg ggtctgaacg aactgcgcct cgatgcgcgc
    10141 gtggccgaag tgctgccgct gcgtcgaacc cctgccggaa taccggtcgc cagctgtgtg
    10201 ctggcgcacg aatcgaaaca agtcgaagcg ggcctgaccc gcgaggtttc ggtggaagtg
    10261 caggcggtgg cgctgggcga tctggcggcg gtgctggctg tggcggttcc cgggagtgcg
    10321 ctgcgcgcca ccggattcct cgccgcgaag agcctgcgca gccgaactcc ggttctgcac
    10381 ctgaacacta tcgaatttct cgaaggaaac tgaaaatggc cttcaagccc aaatccaagt
    10441 tcaagaagaa ggacgatcgc ggcggtcgtg gtctgttcaa gcgtcgcaag ttctgccgct
    10501 tcaccgcgga gaagatcgag gaagtcgact acaaggatgt ggacatcctc aaggacttca
    10561 tcaccgaaaa cgccaagatc atgccggcgc gcatcaccgg caccaaggcg ggttaccagc
    10621 gtcagctgtc ggtcgcggtc aagcgcgccc gcttcctggc cctgatgcct tacaccgacc
    10681 tgcaccagta agaggagaac gaacccatgc agatcattct tctcgagaaa gtcgtgaacc
    10741 tcggtggtct cggcgacgtc gtcaaggtca aggacggcta cgcccgcaac ttcctgatcc
    10801 cgcagggcaa ggcgaagcgc gcgacccagg ccaatctggc cgagttcgaa gcgcgtcgtg
    10861 ccgagctcga gcgcgtccag gccgaaaagc tggctgcggc ccaggagctg gcgggcaagc
    10921 tcgaaggcgt cagcgtcgaa gttgctcgca aggcgggcat ggacggccgt ctgttcggct
    10981 cggtcggcaa tgccgacatc tccgaggcgc tcgccgccaa gggcttcgac atcgagcgtt
    11041 ccgccatccg catgccggac ggcccgctca agcaggtcgg cgagacccag ctcgaagtgt
    11101 cgctgcacgc cgacgtgctc gcgaacatca ccgtcgtggt ggctgccgag cagtaatcgc
    11161 gatctgtacg tgcatcacga agagggctgc cgctggcagc cctttttgtt tcctgcgccc
    11221 ctgcggcgcg ttcccttcgc caacgtctcg cggtccggct cgctacaatg tgcggtccgg
    11281 ccccgatggt gcatccgatg agttccgcgt cttcccgttt ctccgatttc tccgccgatc
    11341 cgcagctcgc gcagatcaag ctgccgccgc actcgctgga ggccgagcag tcgctgatcg
    11401 gcggcatcct gctcgacaac caggcttggg agcgtgtcgc cgacctggtg aacgaggccg
    11461 atttctatcg cgacgaccac cgccgcatct atcgccacat caccaagctg atcgaccagg
    11521 ggcgaccggc ggatgcggtc accgtgttcg agtcgctgga gaagaacggc gaggccgagc
    11581 aggccggcgg tctcgcctac atcgccgagc tcgccaacaa cacgccgtcg gcggcgaaca
    11641 tccggcgcta cgcggagatc gtccgcgagc gcgcgatcct gcgcaagctc gtggcggtgg
    11701 gagacgagat cgctgccagc gcgctcagtc cgtcgggcaa ggacgccaag gcgctgctcg
    11761 acgaggccga ggccaaggtc ttcgagatcg ccgaggccgg cgcgcgccac gccagcggtt
    11821 tcgtctcgat ccagccaatc ctcaagcagg tcgtggatcg cgtgcaggaa ctctacgatc
    11881 gcgacaaccc ctccgaggtc accggcgtgc cgaccggcct ggtcgacctc gacgagaaga
    11941 cctccggcct gcagccgtcc gacatgatca tcgtcgccgg ccgtccggcg atgggcaaga
    12001 ccaccttcgc gctcaacctt gccgagcaca tcgcgatcga gcagcgcctg ccggttgcga
    12061 tcttctcgat ggagatgccg ggcacgcagc tcgcgactcg cttcatctcc tcggtgggtc
    12121 gcatcgacat gcagaagatc cgcagtggac gcctgaccga cgacgactgg cagcgtctga
    12181 ccatggcgat gggcaagctc tacgacgcgc cgctctacat cgacgagacg cccggcctca
    12241 acccgatcga cctgcgcgcc cgtgcgcgtc gcctggcgcg ccagtgcggc aggctcggtc
    12301 tgatcgtgat cgactacctg cagctgatga gcggcacgcg cgagggcgac aaccgtgcct
    12361 ccgagctgtc cgagatctcg cgttcggtaa agtcgctggc caaggagttg aacgtgccga
    12421 tcatggcgct ttcccagctc aaccgcagcc tcgagcagcg tcccaacaag cgcccggtga
    12481 tgtccgatct gcgcgagtcc ggtgccatcg agcaggacgc ggacatcatc atgttcatct
    12541 atcgcgacga ggtctacaac cccgattcgc ccgacaaggg gacggccgaa ctgatcatcg
    12601 gcaagcatcg taacggtccc accggcaccg tgcgcctcac cttcatcggg gagaatacgc
    12661 gcttcgagaa ctacgccgcc gcccccggca tgtactgagc cctatcgcag gatggtcgcg
    12721 attttgcggt ttgcgcagga agatcggcgg tcgtcaccaa gcgattgttc ctgcgtcggg
    12781 ccctgcggga ggcccgtaga gacgctggcg ccagaggtgt gttgtgctcg gtgatcggtc
    12841 ccggggtccc gtcggcgttg ctcgcatgct tccatcggaa tctttgttgt tttttcttga
    12901 cgtcaggatt tggttgtcta taatgcgcac ctcttcc
//
LOCUS       DAOQBS010000086        12869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_3953_length_12869_cov_15.390510, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000086 DAOQBS010000000
VERSION     DAOQBS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12869
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3953_length_12869_cov_15.390510"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKN25_10390"
     CDS             <1..287
                     /locus_tag="PKN25_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HNV91349.1"
                     /translation="LNLTAADTVIHYDPWWNPAAENQATDRAHRIGQDKPVFVFKLVC
                     AGSIEEKILALQERKAALAESVLSEDADALAKFGEADIAALLAPLPADAR"
     gene            complement(515..1183)
                     /locus_tag="PKN25_10395"
     CDS             complement(515..1183)
                     /locus_tag="PKN25_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV91350.1"
                     /translation="MKKIIVTLSAVALAVSGLSGCANMNETQRDTGMGAAIGAVAGGL
                     IGAATAGGNKGKSAATGAAIGAALGAGGGYLWSQNMQKQRAEMEQATAGTGIGISQTT
                     DNQLKVDIPADVSFDVGRHAIKPNMRPVLDRLASTLNQHPVTTVTIIGHTDSTGSDAV
                     NDPLSINRAAATRDYLVQRGVSAQRIAVDGRGSRQPVADNSTASGRAMNRRVEIFIAE
                     PAQR"
     gene            complement(1318..1701)
                     /gene="crcB"
                     /locus_tag="PKN25_10400"
     CDS             complement(1318..1701)
                     /gene="crcB"
                     /locus_tag="PKN25_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNV91351.1"
                     /translation="MNLAAFAAVGAGAACGAWLRWGLGLWLNPVFALVPLGTLMANLL
                     GGFLMGVVLAAVQAWPTMSPALKLLLTTGLLGGLTTFSTFSAESFHLLQRGAIGWLGA
                     HLVVHVAGSVLMTWAGYVLFNALRA"
     gene            1928..3670
                     /locus_tag="PKN25_10405"
     CDS             1928..3670
                     /locus_tag="PKN25_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNV91352.1"
                     /translation="MKPEKKPAAPAAEAPVVSNFIRSIIDEDNRNGKWGGRVETRFPP
                     EPNGYLHYGHAKSICLNFGLAESYGGVCHMRFDDTNPEKEEQEYVDAILEAVQWLGFE
                     WGEHLYFASDYFDKMYACAISLIKAGKAYVDSQSAEEMRVNRGTLTEAGKNSPYRERS
                     VDENLELFARMRAGEFAEGTHILRAKVDMASPNINLRDPAIYRIRHATHHRTGDAWHL
                     YPMYTFAHPIEDAIENITHSVCTLEFEDQRPFYDWLLDALATEGHFPRPLPQQIEFAR
                     LNLTYVVLSKRKLIQLVNEGHVDGWDDPRLPTLVGARRRGFTAAGFRRFAERIGVSKA
                     ESWIDMSVLEECMRDDLNEAAQRRVAVLDPLKLVLTNYAEGASELCQAPNHPLEPELG
                     KRDMPFSRELWIEREDFMEEPVKGFHRLYPGNMVRLRYGFVVKCTGCEKDADGKVTAV
                     LAEYMPDSKSGTPGADDYKVKGNLHWVSVAHGFETEVRIYDRLFAHPHPGQRREGDAP
                     DFERDFLDDINPDSKRVITAYLEPALKDARPEDRFQFERHGYFVADRVDSKPGAPVFN
                     RTVTLKDSWAKGGK"
     gene            3725..4873
                     /locus_tag="PKN25_10410"
     CDS             3725..4873
                     /locus_tag="PKN25_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV91353.1"
                     /translation="MKTLRRLFVLALVAALVGAGIWWMTRPQPIEVAVHEVARGTVEA
                     TLTNTRAGEVEACQRAKMATIAGGRIEWLGVEEGDRVEAGQVLMRLWHGDLDARAAVV
                     RAQLATARQRVQEACAVAANAEREAARQAQLVKRGFVSASAEERARTEARARRAACDT
                     AQADIATAQAQLVATETERQRLVLVAPFAGTVAKISGELGEYATPSPPGVPMPPAIDL
                     IDDSCLYVKAPMDEIDAPKIRPGLPVRITLEAIPGKVFEGRVRRVAPYITAVEKQART
                     VAVDVDFADPAEAHGMLVGYSTDVEIVLATRAEVLRVPTGALREGGKVLVVEGDTLVE
                     RTLRTGVANWEFTEVVSGLAAGERIVTSLEREGVMAGAKVALRPEEAR"
     gene            5208..5903
                     /locus_tag="PKN25_10415"
     CDS             5208..5903
                     /locus_tag="PKN25_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV91354.1"
                     /translation="MAQIELEHIDRIFKLGDSEVHALRDITLRIEPGEYVAVMGPSGS
                     GKSTLLNLLGLLDRPDAGHYRLEGRDVTTLSADEQAAVRSARIGFVFQSFHLVPRLSA
                     AENIGLPLVLAGIPVAERAARIAQALQDFGLEGRARHRPDELSGGQRQRVAIARATIM
                     RPAMLLADEPTGNLDRATGHEVTALLEALNAAGTTLIVVTHDPSMGERAHRRILMEDG
                     AIRQDLRTAAPPP"
     gene            5900..7108
                     /locus_tag="PKN25_10420"
     CDS             5900..7108
                     /locus_tag="PKN25_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV91355.1"
                     /translation="MSPADTLRFATRAATAWPLRTALMMLAMSIGVAAVVVLTALGDG
                     ARRYVVNEFSALGASLLIVLPGRTETGGVNAGSFVSSTPRELTNADAAALLRLPLVQR
                     VAPLAVGNSEIAVGGRLREVTVVGTSADFLELRGLKIGHGGFLPREDFNRASAVAVIG
                     DALRSELFPGQSAVGRMIRVGDTRLRVIGVLTPSGRGLGMTTDELVLVPVATAQAMFD
                     TSGLFRIFVEARGREALPATQRQIEERLRARRDDELDFTVITQDAVLGTFDRILGALT
                     LGVAGIAAISLAVAGILVMNVMLVAVTQRTAEIGLLKALGARAGTIRAAFLAEAALLS
                     VAGALAGFALGHAGAWGVRLAFPQLPAWPPDWAVIAALATALGTGVLFGVLPARRAAR
                     LDPVQALSKR"
     gene            7114..8313
                     /locus_tag="PKN25_10425"
     CDS             7114..8313
                     /locus_tag="PKN25_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV91356.1"
                     /translation="MRWRDALHLALRAITAHRLRSLLTLLGIGVGIAAVILLTSIGEG
                     LHRFVLAEFGQFGTNVINLHPGRQGARGGPPGLPSTARDLTLDDADALARLPHVRHVT
                     GSVSGNAEVRAQGRVRRSTVLGVGPQMQEVYSMRVRVGQFLPPDEAASARAFVVLGPK
                     LARELFGSTIALGEHVEIGAERFRVVGVMEEKGQFLGIDLDDAAYIPVVRGMALYQRD
                     GLMEIALTYDPEAPAARVAEAVKKRMIARHGREDFTVLTQEDMLATLSNILDLLTAAV
                     GALGAISLLVGGVGIVTIMSIAVTERTGEIGLLVALGARRRTILALFLGEAVVLAGIG
                     GLLGLLVGAGLAQLVGLLVPAMPVATPWRYALAAEGVAIVVGLAAGVLPARRAARLDA
                     VEALRAE"
     gene            complement(8446..9900)
                     /locus_tag="PKN25_10430"
     CDS             complement(8446..9900)
                     /locus_tag="PKN25_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNV91357.1"
                     /translation="MSAQVHALAAPTPRTAAAAATARAAPIAAAVSNPRAAPAAERFA
                     PMSFKVFTARDLDRIPQLARLSPEQRFEMKVVAAVLPFRVNQYVIDELIDWADVPRDP
                     IFQLTFPQRGMLASEHYERIARLIEGDADKDALEAAIAEVRHALNPHPADQMQMNMPL
                     DEHGKRIDGLQHKYRETVLFFPSQGQTCHAYCSFCFRWAQFVGDKELRIASSEARVLH
                     DYLRTHTEVTDLLVTGGDPMVMKTRHLREYLEPLLRPEFDHIQTIRIGSKALTFWPHR
                     FLGAEDADDLMRLLRQLVEAGKHVALMAHYNHWKELETDAAHAAIRRIRATGAVIRAQ
                     GPLIAHINDDPAAWARLWKTEVRLGLVPYYMFVERDTGARHYFEVPLARAWEIYQQAI
                     QQVSGLARTARGPSMSASPGKVEIQGVTEIAGEKVFVLRFIQGRNPDWVQRPFFARFD
                     EKATWLDQLAPAFGEAKWFWQDEYEAIRAERLVG"
     assembly_gap    10242..10341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10678..11532
                     /locus_tag="PKN25_10435"
     CDS             10678..11532
                     /locus_tag="PKN25_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNV91358.1"
                     /translation="MDFRNGRESDNVEDRRGQGGRGLRGGGKIGIGTIVLALVAMYFG
                     IDPSVVLDTAMTDPAPVESTQTRPAAENAQARFVSMVLADTEDTWRTIFQQGGATYRE
                     PKLVLYTGATRSACGVGQAEMGPFYCPADAKVYLDLAFFDELHHRFGAPGDFAQAYVI
                     AHEIGHHVQNLLGISDKVQQARTRVSEREANQLSVRLELQADCLAGIWAHHANRSRSV
                     LEAGDIEEGLAAATAVGDDHIQKRSQGYAVPDSFTHGSAEQRRRWFAIGLQQGQFKSC
                     DTFSAPRL"
     gene            complement(11785..>12869)
                     /locus_tag="PKN25_10440"
     CDS             complement(11785..>12869)
                     /locus_tag="PKN25_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNV91359.1"
                     /translation="LITGLDLVEQMIRVAYGEKLPLAQADVKIDGWAMECRINAEDPF
                     RGFLPSTGRLIKFQPPAEGNGVRVDTGVYEGGEISMFYDSMIAKLIVHGKDRQDAIER
                     MRDALNGFVIRGISSNIPFQAALLQHPRFCSGHFNTGFIAEEYPKGFDASMVPHDDPA
                     LLAAVATFARMRYIERAVQIEGQLAGHGRKVASEWVVVMGGKQYAMRTAAIEGGLAVT
                     CEGRTYNIVSDWKFGELLFAGTVNGAPIGLQIERRGLRYRISHFGLQVEPVVMTVRAA
                     HLLSLMPAKLPPDLSKFLLSPMPGLLREVAVTEGQDVKAGEKLAIIEAMKMENVLKAE
                     QDGKVKKIVAKPGASLSVDEVIIEFE"
BASE COUNT         1938 a         4452 c         4491 g         1888 t
ORIGIN      
        1 gcctcaacct caccgccgcc gacaccgtga tccactacga cccgtggtgg aaccccgcgg
       61 ccgagaacca ggccaccgac cgcgcccacc gcatcggcca ggacaagccg gtgttcgtgt
      121 tcaagctggt gtgcgcgggc agcatcgagg agaagatcct cgccctgcag gagcgcaagg
      181 ccgccctcgc cgagagcgtg ctgtcggagg acgcggacgc gctcgccaag ttcggcgagg
      241 ccgacatcgc cgccctgctc gcgccgctcc ccgcggacgc gcgctgagca agagcggaag
      301 ccgggcaaag ctgtcctgca ggagcggcgc aagcctcggt gccggcgctc ctccgcgccg
      361 aagcgaccga acgcacgagt cgccacttgc gccgcccccg caatgagcac ggggctccag
      421 gagccccgtg ggagcgacgc gagtcgcgat tgaagcggtc gagatggcgc ggcgcgaaat
      481 cccgccgcaa gcgctcaccc gaccccgtcc ggggtcagcg ctgcgccggc tccgcgatga
      541 agatctccac gcggcggttc atcgcacgac cggaggccgt cgagttgtcg gcgacgggct
      601 ggcgcgagcc gcgcccatcg accgcgatgc gctgcgcgga cacgccacgc tgcaccagat
      661 aatcgcgggt ggcggcggcg cggttgatcg atagcggatc gttcaccgca tccgagccgg
      721 tgctgtcggt atggccgatg atggtcaccg tggtcaccgg atgctggttc agcgtgctgg
      781 ccagacggtc gagcaccgga cgcatgttgg gcttgatcgc gtggcggccc acgtcgaagg
      841 aaacgtccgc gggaatgtcg accttaagct ggttgtcggt cgtctggctg atgccgatgc
      901 cggtgcccgc ggtcgcctgc tccatctcgg cgcgctgctt ctgcatgttc tgcgaccaca
      961 ggtagccgcc gcccgccccg agtgcagcgc cgatcgcagc accggttgcg gcgctcttgc
     1021 ccttgttgcc gcccgcggtg gcggcaccga tgaggccgcc ggccacggcg ccgatggcgg
     1081 ctcccatgcc cgtgtcgcgc tgggtctcgt tcatgttcgc gcagccggaa agaccgctca
     1141 cggcgagcgc gaccgcggaa agggtgacga tgattttttt cattggatgg gctccgttgc
     1201 gttgaggggg tgccactggg gatgcagaac ggcgtgaatt atcgatgtaa cgctcatcca
     1261 ctctagccca tcggagtgaa acagaaaatg acgcggccgc ccgccttgcg gcgcaggtca
     1321 cgcacgcagg gcattgaaaa gcacgtatcc cgcccaggtc atcagcacgg atccagccac
     1381 atgcacgacc aggtgggcac ccagccagcc gatcgcgccg cgctgcaaca ggtggaagga
     1441 ctcggcggaa aaggtggaaa aggtcgtcag cccgccgagc aggccggtgg tgagcaggag
     1501 cttgagcgcc ggcgacatcg tcggccaggc ctgcaccgcc gcgagcacta cgcccatgag
     1561 gaaaccgccc agcaggttcg ccatcagggt gccgagcggg acgagcgcga acaccgggtt
     1621 gagccacagc cccaatcccc agcgcagcca cgcaccgcac gccgcccccg ccccaaccgc
     1681 tgcgaatgcc gccagattca tcgcccgtgc tccatcccgc ctccctgctg ccgcccaccg
     1741 gccgcacgat tctacccctc cgcgggcatc gcgccccgaa cgccgcaccc cgtgaccggc
     1801 ccgctccggc gagcccgcac gcccgatcgg ctacaatcgg tccattgcgc cgcccgcctc
     1861 ggggtccggc cccagctgca cacgaacccc caccatcccg ccgaactttc cgacccctgc
     1921 cttcccgatg aaacccgaaa agaaacccgc cgcacccgcc gccgaagcgc ccgtcgtctc
     1981 caacttcatc cgcagcatca tcgacgagga caaccgcaac ggcaaatggg gcggccgcgt
     2041 cgagacacgc ttcccgcccg agcccaacgg ctacctgcac tacggccacg ccaagtcgat
     2101 ctgcctgaac ttcggcctgg ccgagtccta cggcggcgtc tgccacatgc gcttcgacga
     2161 caccaacccc gagaaggaag agcaggaata tgtcgacgcc atcctcgagg cggtgcagtg
     2221 gctcggcttc gagtggggcg agcacctgta ctttgcgtcg gactacttcg acaagatgta
     2281 cgcctgcgcc atcagcctga tcaaggccgg caaggcctat gtcgattcgc agtcggccga
     2341 ggagatgcgc gtcaaccgcg gcaccctgac cgaggcgggc aagaacagcc cgtaccgcga
     2401 gcgcagcgtc gacgagaacc tcgagctctt cgcgcgcatg cgcgccggcg aattcgccga
     2461 gggcacgcac atcctgcgcg ccaaggtcga catggcgagc cccaacatca acctgcgcga
     2521 cccggcgatc taccgcatcc gccacgccac ccaccatcgc accggcgacg cttggcacct
     2581 gtacccgatg tacaccttcg cgcatccgat cgaggacgcg atcgagaaca tcacccactc
     2641 ggtgtgcacg ctcgagttcg aggaccagcg cccgttctac gactggctgc tcgacgcgct
     2701 cgccaccgaa ggccacttcc cgcgcccgct gccgcagcag atcgagttcg cccgcctcaa
     2761 cctgacctac gtggtgctgt ccaagcgcaa gctgatccag ctggtgaacg aaggccatgt
     2821 cgacggctgg gacgacccgc gcctgcccac gctggtcggc gcccgccgcc gcggcttcac
     2881 cgccgccggc ttccgccgct tcgccgagcg catcggcgtg tccaaggccg agtcgtggat
     2941 cgacatgagc gtgctggaag agtgcatgcg cgacgatctc aacgaggccg cccagcgccg
     3001 cgtggcggtg ctcgacccgc tgaagctcgt ccttaccaac tacgccgaag gcgcctccga
     3061 gctgtgccag gcacccaacc acccgctcga gcccgagctc ggcaagcgcg acatgccctt
     3121 ctcgcgcgag ctgtggatcg agcgcgagga cttcatggaa gagcccgtca agggcttcca
     3181 ccgcctctat ccgggcaaca tggtgcgcct gcgctacggc ttcgtcgtca agtgcaccgg
     3241 ctgcgagaag gacgccgacg gcaaggtcac cgcggtgctc gccgagtaca tgcccgactc
     3301 gaagtccggc acgccgggcg ccgacgacta caaggtcaag ggcaacctgc actgggtgtc
     3361 ggtggcgcac ggttttgaaa ccgaggtgcg catctacgac cgcctcttcg cccaccccca
     3421 tcccggccag cgccgcgaag gcgacgcgcc cgacttcgag cgcgacttcc tcgacgacat
     3481 caaccccgac agcaagcgcg tcatcaccgc ctacctggag ccggcactga aggacgcccg
     3541 gcccgaagac cgcttccagt tcgagcgcca cggctacttc gtcgccgacc gcgtggactc
     3601 gaagcccggc gcgccggtgt tcaaccgcac ggtgacgctg aaggattcgt gggccaaggg
     3661 cgggaagtga gatcgcatcc ttcgcgcgcg gcgaccagac cttgaccgct ccggcactcc
     3721 actcatgaag accctccgcc gcctcttcgt tctcgcactc gtcgcggcgc tggtgggcgc
     3781 ggggatctgg tggatgaccc gcccgcagcc gatcgaggtg gcggtgcacg aggtggcccg
     3841 cggtacggtc gaggccacgc tgaccaacac ccgcgccggc gaggtcgagg cctgccagcg
     3901 cgcgaagatg gcgacgatcg cgggcgggcg gatcgagtgg ctgggcgtgg aggaaggcga
     3961 ccgcgtcgag gccggacagg tgctgatgcg gctgtggcat ggtgacctcg acgcgcgcgc
     4021 ggcggtcgtg cgcgcccagc tcgccaccgc ccgccagcgc gtgcaggagg cgtgcgccgt
     4081 ggcggccaac gccgagcgcg aggccgcgcg ccaggcgcag ctggtgaagc gcggcttcgt
     4141 gtccgccagc gccgaggaga gggcccgcac cgaggcgcgt gcccgccgcg ccgcctgcga
     4201 caccgcgcag gccgacatcg ccaccgccca ggcccagctc gtcgcgaccg aaaccgagcg
     4261 ccagcgcctg gtgttggttg cgcccttcgc cggcaccgtg gccaagatca gcggcgagct
     4321 gggcgaatac gcgacgccct ccccgcccgg cgtgccgatg ccgccggcga tcgacctgat
     4381 cgacgactcc tgcctgtacg tgaaggcgcc gatggacgaa atcgacgcac cgaagatccg
     4441 gcccgggctg ccggtacgca tcacgctcga ggccatcccc ggcaaggtct tcgaaggccg
     4501 cgtgcgccgt gtcgcgccgt atatcaccgc ggtggagaag caggcgcgca cggtggcggt
     4561 ggatgtggat ttcgcggacc cggccgaggc gcacgggatg ctggtgggct acagcacgga
     4621 cgtggagatc gtgctcgcga cacgtgcgga ggtgctgcgc gtcccgaccg gggccttgcg
     4681 ggagggcggc aaggtgctgg tggtcgaggg cgacacgctg gtcgaacgca cgctgcggac
     4741 gggcgtggcg aactgggagt tcaccgaggt ggtgtcggga ctggctgcgg gggagaggat
     4801 tgtgacgtcg ctcgagcggg agggggtgat ggcgggggcg aaggtggcgc tgcggccgga
     4861 agaggcgcgg taggcgctgg atcgaagtgg gcgccccggc tcccggatcg cgacttgcgt
     4921 cgctcccaca tccctccccg agcgccgcgg tgtcgccgcg tgcacgccag tcggcgcacg
     4981 cattcccggg cgtcgcagcg tcgccgcttc cggtccaatc ggcgccagcc tcgccaggcg
     5041 ccgcgtctcg cctgtgggag cgacgcaagt cgcgattgcc gccgccgagc gggcaaggtc
     5101 ttctttcatt gtccgctcgc ccgacacccc atgcagggcg ctgaaaggtg cctcatgcca
     5161 cctcacggaa gccacaccgg cctgaattga agctgcaaca gaacaccatg gcccagatcg
     5221 aactcgagca catcgaccgc atcttcaagc tcggcgacag cgaggtgcac gccctgcgcg
     5281 acatcacgct gcgcatcgag cccggcgagt acgtcgcggt catgggaccg tcgggttccg
     5341 gcaaatcgac gctgctcaac ctgctcggtc tgctcgaccg ccccgacgcc ggccattacc
     5401 ggctcgaagg ccgcgacgtc accacactct cggccgacga gcaggccgcg gtgcgcagcg
     5461 cacgcatcgg cttcgtgttc cagagcttcc acctcgtccc ccgcctcagc gcggccgaga
     5521 acatcggcct gccgctggtg ctcgccggca tccccgtcgc cgagcgcgcg gctcgcatcg
     5581 cgcaggcctt gcaggacttc ggcctcgagg ggcgcgcccg gcaccgcccc gacgaactct
     5641 ccggcggcca gcgccagcgc gtcgcgatcg cgcgtgccac catcatgcgg ccggcgatgc
     5701 tgctcgccga cgagcccacc ggcaacctcg accgcgccac cggccacgag gtcaccgcgc
     5761 tgctcgaggc gctcaacgcc gccggcacca cgctgatcgt cgtcacccac gacccttcga
     5821 tgggcgagcg cgcacaccgc cgcatcctga tggaggacgg cgcgatccgt caggacctgc
     5881 gcaccgccgc accgccccca tgagccccgc cgacaccctg cgcttcgcca cccgcgccgc
     5941 caccgcgtgg ccgctgcgca ccgcgttgat gatgctggcg atgtcgatcg gcgtcgccgc
     6001 ggtggtggta ctcaccgcgc tcggcgacgg cgcgcggcgc tacgtggtga acgagttctc
     6061 cgcgctcggt gccagcctgc tgatcgtgct gcccggacgc accgagaccg gtggcgtcaa
     6121 cgcgggcagc ttcgtcagca gcacgccgcg cgagctcacc aacgccgacg ccgccgcgct
     6181 gttgcgcttg ccgctggtgc agcgcgtggc gccgctcgcg gtgggcaact ccgagatcgc
     6241 cgtcggcggc cggctgcgcg aggtcacggt cgtcggcacc agcgccgact tcctcgagct
     6301 gcgcggtctg aagatcggcc acggcggctt cctgccgcgc gaggacttca accgcgcctc
     6361 ggcggtggcg gtgatcggcg acgcactgcg cagcgaactc ttccccggac agagcgcggt
     6421 gggccgcatg atccgtgtcg gcgacacccg gctgcgcgtg atcggcgtgc tcacgccatc
     6481 cgggcgcggg ctgggcatga ccaccgacga gctggtgctg gtgccggtgg cgaccgcgca
     6541 ggcgatgttc gacaccagcg ggctgttccg catcttcgtc gaggcgcgcg ggcgcgaggc
     6601 cttgcccgca acgcagcgcc agatcgagga gcgcctgcgc gcgcgccgcg acgacgagct
     6661 cgacttcacc gtgatcaccc aggacgccgt tctcggcacc ttcgaccgca tcctcggggc
     6721 gctcaccctg ggcgtggccg gcatcgccgc gatcagcctg gcagtcgcgg gcatcctggt
     6781 gatgaacgtg atgctggtcg cggtcaccca gcgcaccgcc gaaatcggcc tgctcaaggc
     6841 gctcggcgcg cgcgccggca ccatccgcgc cgccttcctc gccgaggccg cgctgctctc
     6901 cgtcgccggc gcgctcgccg gcttcgcgct cggccacgcc ggcgcctggg gcgtgcgcct
     6961 ggctttcccg cagctgccgg cgtggccgcc cgactgggcg gtgatcgccg cgctcgccac
     7021 cgcgctcggc accggggtgc tgttcggcgt gctgcccgcg cgccgcgccg cccggctcga
     7081 tccggtgcag gccttgtcga agcggtagcg gcgatgcgct ggcgcgacgc cctccacctc
     7141 gccctgcgtg cgatcaccgc gcatcggctg cgcagcctcc tgaccctgct cggcatcggc
     7201 gtgggcatcg ccgcggtgat cctgctcacc tcgatcggcg agggtctgca ccgcttcgtg
     7261 ctcgccgagt tcggccagtt cggcaccaac gtgatcaacc tccacccggg ccgccagggc
     7321 gcacgcggcg gcccgcccgg cctgccgtcc accgcgcgcg acctcaccct ggacgacgcg
     7381 gacgcgctcg cccgcctgcc ccatgtgcgc cacgtcaccg gttcggtgag cggcaacgcc
     7441 gaggtgcgcg cgcaaggccg ggtgcggcgc agcaccgtgc tcggcgtggg accgcagatg
     7501 caggaggtgt actcgatgcg ggtgcgcgtg gggcagttcc tgccgcccga cgaggcggcg
     7561 agcgcgcgcg ccttcgtggt gctgggcccc aagctcgcac gcgagctctt cggcagcacc
     7621 atcgcgctcg gcgagcacgt cgagatcggt gccgagcgct tccgcgtggt cggcgtcatg
     7681 gaagagaagg ggcagttcct cggtatcgat ctggacgacg ccgcctacat cccggtggtg
     7741 cgcggcatgg cgctgtacca gcgcgacggc ctcatggaga tcgcgctgac ctacgacccc
     7801 gaggcccccg ccgcgcgcgt ggcggaggcg gtgaagaagc ggatgatcgc gcgccacgga
     7861 cgcgaggact tcaccgtgct gacgcaggag gacatgctgg cgacgctgtc gaacatcctc
     7921 gatctgctca ccgcggcggt gggtgcgctc ggggcgatct cgctgctggt gggcggagtg
     7981 ggcatcgtca ccatcatgag catcgcggtc accgagcgca ccggagagat cggcctgctg
     8041 gtggccctgg gcgcacgtcg gcgcaccatc ctcgcgctct tcctcggcga ggcggtggtg
     8101 ctggcgggca tcggcggcct gctcggcctg ctcgtcggcg caggccttgc ccagctggtc
     8161 gggctgctgg tgccggcgat gccggtggcc acgccctggc gatacgcgct cgccgccgag
     8221 ggggtcgcca tcgtggtcgg gctcgccgcc ggggtcctgc ccgcacgcag ggcggcacgg
     8281 ctcgacgcgg tcgaggcctt gcgcgcggag tgagccatgc ggcgcgcgcg cccgttccag
     8341 cgccaccctc gcacgcacgt cgttcctgca aaaccaccgc gcttccggga gcgcctggag
     8401 ccaagcgagt cgcgagtcgg acgctgacga agcgccgccc cgccctcagc cgacgagcct
     8461 ttccgcgcgg atcgcctcgt attcgtcctg ccagaaccat ttcgcctcgc cgaaggcggg
     8521 cgcgagctgg tcgagccagg tggccttttc gtcgaaccgg gcgaagaacg ggcgttgcac
     8581 ccagtcgggg ttgcggccct ggatgaagcg cagcacgaag accttctcgc cggcgatctc
     8641 ggtgacgccc tggatctcga ccttgccggg gctggcactc atgctcgggc cgcgggcggt
     8701 gcgggcgagg ccggagacct gctggatggc ctgctggtag atctcccagg cgcgcgcgag
     8761 cggaacctcg aagtaatggc gggcgccggt gtcgcgctcg acgaacatgt aataggggac
     8821 gaggccgagg cggacctcgg tcttccacaa gcgcgcccag gcggcggggt cgtcgttgat
     8881 gtgggcgatg agcggacctt gtgcgcggat gacggcaccg gtggcgcgga tgcggcggat
     8941 ggcggcgtgg gcggcgtcgg tctcgagctc cttccagtgg ttgtagtgcg ccatcagcgc
     9001 gacgtgcttg ccggcttcga cgagctgccg gagcaggcgc atgagatcgt cggcatcctc
     9061 ggcgccgagg aagcggtgcg gccagaaggt gagcgccttg gagccgatgc ggatggtctg
     9121 gatgtggtcg aactcggggc gcagcagggg ttcgaggtat tcgcgcaggt gacgggtctt
     9181 catgaccatg gggtcgccac cggtgacgag caggtcggtg acctcggtat gggtgcgcag
     9241 gtagtcgtgc agcacgcggg cttcggagct ggcgatgcgc agctccttgt cgccgacgaa
     9301 ttgcgcccag cggaagcaga agctgcaata ggcgtggcag gtctggccct ggctggggaa
     9361 gaagagcacg gtctcgcgat acttgtgctg caggccgtcg atgcgcttgc cgtgctcgtc
     9421 gagcggcatg ttcatctgca tctggtcggc ggggtgcggg ttgagggcgt ggcggacctc
     9481 ggcgatggcg gcctcgagcg cgtccttgtc cgcgtcgcct tcgatgaggc gggcgatgcg
     9541 ctcgtagtgc tcggacgcga gcatgccgcg ctgcgggaag gtgagctgga agatggggtc
     9601 gcgggggacg tcggcccagt cgatgagctc gtcgatgacg tattggttga cgcggaaggg
     9661 cagcacggcg gccacgacct tcatctcgaa gcgctgctcg ggagaaaggc gcgcgagctg
     9721 cgggatgcgg tcgagatcgc gggcggtgaa gaccttgaag gacatcgggg cgaagcgctc
     9781 ggcggcgggc gcggcgcgcg ggttcgacac tgcggcggcg atgggggccg ctcgcgcggt
     9841 cgcggcagcg gcggctgtgc gcggggtcgg ggccgcgaga gcatgcactt gtgcgctcat
     9901 gttcgtctcc ttggtctggt tgtgctgggg gtctgccggt gccgcgggtt gcggccgcgc
     9961 accggttgcg gttcggcagc gcgtgcggtg gccgggcaaa cggcggcgcc cgaggcaagc
    10021 atcggccgac ggcggccagg ctcccggcat ccgtcgacac cccggagtgg ggaatcgctt
    10081 ggggatgtga tggtatgtca caaagttgtt ttgtgcaaat ctaataaagg catactcgga
    10141 ggaagctctg ctgccggccg cggcgcccgt ggccgcgccg gttctcgacc gccgcgatcg
    10201 cgacttgcgt cgctcctacg acaccatcac agttgcatcg gnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn ngaccgccgc gatcgcgact tgcgtcgctc ctacgacacc
    10381 atcacagttg catcggcatc ggcatcggca tcggcatcgg catcggcatc ggcatcggca
    10441 tcgggcgatg cgtggcgcgc tgcccgggcg acgatccaag gcgatggcct tgatttggtt
    10501 cacgtaggag cgacgcaagt cgcgattccg gccttcgggc agcgcggcgt gggcggaagc
    10561 gccgcattcg cgacttgcgt cgcgcctgcc ggagagccgc tgcgcggatg gcttgcgctt
    10621 gccttgcccc ctcggcactc actaagctga agaggttccc cacgaatgga gcgcaccatg
    10681 gacttccgca acggcaggga gagcgacaac gtcgaggacc gccgcggaca aggcgggcgc
    10741 ggcctgcgcg gcgggggcaa gatcggcatc gggacgatcg tgctcgcgct ggtggcgatg
    10801 tacttcggga tcgatccgag cgtggtcctc gacacggcca tgaccgaccc cgcgccggtc
    10861 gaatccaccc agacccgccc cgccgccgag aacgcgcagg cgcgcttcgt gtcgatggtg
    10921 ctggccgaca ccgaggacac ctggcggacg atcttccagc aggggggcgc gacctaccgc
    10981 gagcccaagc tcgtgctcta caccggcgcg acgcgcagcg cctgcggggt cgggcaggcg
    11041 gagatggggc cgttctactg tccggcggac gccaaggtgt atctcgacct ggccttcttc
    11101 gacgagcttc accaccgctt cggcgcgccc ggcgactttg cccaggcgta cgtgatcgcg
    11161 cacgagatcg gccaccacgt gcagaacctg ctgggcatct cggacaaggt gcagcaggcg
    11221 cgcacgcggg tgtcggagcg cgaggcgaac cagctctcgg tgcgcctgga actgcaggcg
    11281 gattgcctcg cgggcatctg ggcccaccac gccaaccgca gccgcagcgt gctcgaggcc
    11341 ggcgacatcg aggaaggcct cgccgccgcc accgcggtgg gcgacgacca catccagaag
    11401 cgcagccagg gttacgcggt gccggacagc ttcacccacg gcagtgccga gcagcgccgg
    11461 cggtggttcg cgatcggatt gcagcagggg cagttcaagt cctgcgacac gttcagcgcg
    11521 ccgcggttgt agttacgccg tcgccgcaaa ggcacggcgc catcgcccgg gagcccgtcc
    11581 agggcgatga cgccgattgt tttcgcgtag gagcgacgca acacatcgca ccgacgtcac
    11641 ccgggagccc gtccgcggcg cgcgttccgg tcccgcggtg gccggacaaa aaagaggcct
    11701 gcttgcgcag gcctcgtgcc gacaggcacc cgggcgtggc ccgggtctcc ctccccatcg
    11761 atcaaaacgg ttttcgggtg gcgatcactc gaactcgatg atgacctcgt ccaccgacag
    11821 gctggcgccg ggcttggcga cgatcttctt gaccttgccg tcctgctcgg ccttgaggac
    11881 gttttccatc ttcatggctt cgatgatggc gagcttctcg ccggccttga cgtcctggcc
    11941 ctcggtcacc gcgacctcgc gcagcaggcc gggcatgggc gagagcagga acttggagag
    12001 gtcgggcggc agcttcgcgg gcatcagcga cagcaggtgg gccgcgcgca cggtcatcac
    12061 gacgggttcg acctgcaggc cgaagtgcga gatgcggtag cgcaggccgc ggcgttcgat
    12121 ctgcaggccg atcggggcgc cgttgacggt gcccgcgaag agcagctcgc cgaacttcca
    12181 gtcggagacg atgttgtagg tccggccttc gcaggtgacg gcgaggccgc cttcgatggc
    12241 ggcggtgcgc atggcgtact gcttgccgcc catgacgacg acccattcgc tggcgacctt
    12301 gcggccgtgg ccggcgagct gaccttcgat ctgcacggcg cgctcgatgt agcgcatgcg
    12361 ggcgaaggtg gcgacggcgg cgagcagcgc ggggtcgtcg tgcgggacca tggaggcgtc
    12421 gaagcccttc ggatactcct cggcgatgaa gccggtgttg aagtggcccg agcagaagcg
    12481 cggatgctgc agcagcgcgg cctggaaggg gatgttggag ctgatgccgc ggatgacgaa
    12541 gccgttgagg gcgtcgcgca tgcgctcgat cgcatcctgg cggtccttgc cgtgcacgat
    12601 gagcttggcg atcatcgagt cgtagaacat cgagatctcg ccgccttcat acacgccggt
    12661 gtcgacgcgc acgccgttgc cttcggccgg gggctggaac ttgatgaggc ggccggtgga
    12721 gggcaggaag ccgcggaagg ggtcttcggc gttgatgcgg cattccatcg cccagccgtc
    12781 gatcttgacg tcggcctggg cgagcggcag cttttcgccg taggcgacgc ggatcatctg
    12841 ctcgacgagg tccagcccgg tgatgagct
//
LOCUS       DAOQBS010000087        12748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4031_length_12748_cov_16.536674, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000087 DAOQBS010000000
VERSION     DAOQBS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12748
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4031_length_12748_cov_16.536674"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKN25_10445"
     CDS             <1..326
                     /locus_tag="PKN25_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNV91360.1"
                     /translation="IAEFGQKAKDGKLSLDDLSGGTFSISNGGVFGSMMSTPIINPPQ
                     SAILGIHATKDRAVVENGQVVVRPINYLAMSYDHRIIDGREAVLGLVTMKEALEDPAR
                     LILDV"
     gene            461..1885
                     /gene="lpdA"
                     /locus_tag="PKN25_10450"
     CDS             461..1885
                     /gene="lpdA"
                     /locus_tag="PKN25_10450"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNV91361.1"
                     /translation="MSKEFDVLVIGGGPGGYVAAIRAAQLGFKTACCESNPYADPKGE
                     PRLGGTCLNVGCIPSKALLHTSHLFEEAGHAFEGQGISVGTPKIDVPKMIARKSGIVD
                     QLTGGIKGLFKKNKVTLLNGHGAFVGKGDAGWQVQVGEELVTAKQVIVATGSAPRHLP
                     GVPVDNKIVCDNVGALDLDAVPKKLAVIGAGVIGLEMGSVWRRLGAEVTVLEAMPEFL
                     SLADQDVAKEALKVFTKQGLKFNLGVTIGEVKVGKKGVSIAYRDKDGAEQKLEADRLI
                     VSVGRVPNTQGLNAEAVGLKLNERGQIEVDDHCKTNLPGVWAVGDVVRGPMLAHKAME
                     EAVMVAELMAGQAGHCNFDTVPWVLYTSPEIAWVGKTEQQLKAAGVAYKVGKIPFLAN
                     GRALGMGDSTGFVKMLADATTDRILGVHIIGANASELISEAVVAMEFAGCSEDLARIC
                     HAHPTLSEVVHEAALACDKRPLHF"
     gene            1989..3104
                     /gene="zapE"
                     /locus_tag="PKN25_10455"
     CDS             1989..3104
                     /gene="zapE"
                     /locus_tag="PKN25_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNV91362.1"
                     /translation="MPHRILNVPQHGMIDAYEALLKTRGYQSDPAQRAAAQRLQGLYT
                     ELVGFKAARQGSRIKQFLNKPKMPRSVYFWGGVGRGKSFLMDCFYDSVPYRRKRRVHF
                     HAFMQEVQNDLRKFNNAPDPLQKVADRISEHTRLLCFDEFHVSDIADAMILGRLLDAL
                     FDRGVIFVMTSNYPPDGLYPNGLMRINFLPTIEKIKQRFDVIEVDHGTDYRLRTLEKM
                     EIYLVPHDDAAKRKMAEDFRRLAASEGRGGSVEILGRNIDVVRQAPGVIWFDFASLCG
                     GPRSQNDYLEIAREHHTVILSNVPKMLVGNASEARRFTWLIDVLYDHRVKLIMSAAVE
                     AHDLYTEGHNAHEFVRTVSRLIEMRTRDYLAEPHRTE"
     gene            complement(3230..5275)
                     /locus_tag="PKN25_10460"
     CDS             complement(3230..5275)
                     /locus_tag="PKN25_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HNV91363.1"
                     /translation="MKKPDTLDDLPTNLSANEHFQSVVERAVSRRGFLKSGLGLSAVT
                     FLSGSLAACTSDDDTPVAESPSAGTPPAPAPAAGPLLGFAAVATSSGDAIVVPAGYSA
                     QIFTPWGSPLFSDSPAWRPDGTNTGEEQARQVGDNHDGMSYFPIDGSNEGLLVMNHEY
                     CNYEYLFGAEFMTPWTADKVSKALNAHGVSVLHVKKNGAGRWEVHIGSPYNRRITGKT
                     PMTLTGPAAGDALLRTTADPSGLNVLGTLNNCANGKTLWNTYLTCEENFNGYFATAAS
                     PAPTRSAAFVRYGISAGGSGYRWHEHEDRFDYAKEPNEANRFGWVVEINPFEPGSTPK
                     KRTALGRFKHENAEMRLAADKRVVVYMGDDQANDYIYKFVSDGVFDASRGLANGNLLD
                     AGKLYVAKFDAGAASGDFMGVGEWLLLDKAANPTLAADARFATQAEVLIHARLAADAV
                     GATKMDRPEWITTHPQTGEVYCALTNNSGRTTTDEANPRAQNRYGQIVRWREAGDDAA
                     AMTFEWDLFVLAGNPVAYPDRQDLRSGSANVSADNTFNSPDGIGFDGAGRLWIQTDGN
                     FSNSGDYAGQGNNQMLVADPESKEIRRFLVGPSGCEITGLAFSPDYRTMFINVQHPGE
                     AGSHPRAPDASMRGSLSMDEYLAQNPLAFSQWPEAGGGRPRSATVVITKDDGGVVGS"
     gene            complement(5408..6154)
                     /gene="gspN"
                     /locus_tag="PKN25_10465"
     CDS             complement(5408..6154)
                     /gene="gspN"
                     /locus_tag="PKN25_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNV91364.1"
                     /translation="MKFATGAAIFAAGVGALLVASAPALLLDAALDHASAGRFRLVLA
                     EGTLWNGQGRLASADSSASLSPITRLRWAFEPSALAGARLRWQLTIDGGAPATLEIDP
                     GGVALQRLAVQLPPATALAAVPHAIARAGWRGVLELNVPALACGWGGRCAGTLQAEWR
                     AGGVDILPGQALGDHRIVAAIAAPVTTLEISALRGRALAVNGKVELSGGQRPRVDLDV
                     GGDPRLLGRLEGMLAELRPTRDGERMRIRF"
     gene            complement(6151..6684)
                     /gene="gspM"
                     /locus_tag="PKN25_10470"
     CDS             complement(6151..6684)
                     /gene="gspM"
                     /locus_tag="PKN25_10470"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HNV91365.1"
                     /translation="MTASSTPALASGRSPALSPIARLRAWYAACAPRERRMLVLAVAV
                     VLGGALISTAEQLAAERERLARSLPAARLAYLGMEQDSLGLAALRERPTPTAPPLAAL
                     PDSIRAAASARGIEAAARIAGDRVEVGGRATLPALVDWLATLHAEQRLRPTRLELQAA
                     GTDGLARFDAMFEVGGQ"
     gene            complement(6691..7866)
                     /gene="gspL"
                     /locus_tag="PKN25_10475"
     CDS             complement(6691..7866)
                     /gene="gspL"
                     /locus_tag="PKN25_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HNV91366.1"
                     /translation="MTTTLMLLAGERWPQDPVADWVLLDAARRPLDHGRSDPRHWPAA
                     DRHELVLAGSLVIRLELELPPAPKREQPQLIRYALESHLARDPDLQHLTVSGRDPANA
                     RVGVLVVARSELATLVATCKALGRPLDAVRSALQLAPPASGEWTLALLPGATAVLRTG
                     LQAGMALDADSTDTLAEWLALQLDATPAEARPTRLEVRSTTGTPIPETPALAARIGVE
                     LREGPTLDWWRLANDAANLLHGEFAPAHGGDGWWRQLRRPALLAAGAAGAWLLVQAAL
                     VAIDGGEERALRERGERLAAEALPGQPLLEPRMQLRRAHERALQVHGQLAAGDLLALL
                     DAALEAGAPQPQALRYEDGRLTLELASAPPADLLVRLDARGLAARAEGSRLLLAARP"
     gene            complement(7863..8855)
                     /gene="gspK"
                     /locus_tag="PKN25_10480"
     CDS             complement(7863..8855)
                     /gene="gspK"
                     /locus_tag="PKN25_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HNV91367.1"
                     /translation="MSARSPAFSRCARRAAQRGAAVVLALLTVSFAAMLAAAALADFG
                     HGLAMLGGRHDQAQAWQLGRAAVDWSRNILADDARRTSTDHFGEIWTTAIPVTAIGSD
                     SADGSVGGAIHDLSGRFNLNDLAPNGRPDTPAIARFTRLLGLVGMDDASANATARALA
                     AHLQGGTAELGTAPAAAPGTAPDGRSGGETNANAGGGRALVETRELVLLGLVPAATFA
                     ALEPHVVAAPPGAPININTASAEVLAAVLDSLSLDVARNLVAARERAWFRNLGDLEGR
                     LPEGAALPAGRVLDVRSRYFLVTISAAYGDALLNLQAMLDRESNWPAILWYHTP"
     gene            complement(8818..9489)
                     /locus_tag="PKN25_10485"
     CDS             complement(8818..9489)
                     /locus_tag="PKN25_10485"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HNV91368.1"
                     /translation="MQPYRTPPPALAARRRSARRRGAGLTLIELMVAMALLGVIGVLT
                     WRATSQLVDGRERIGAELARWQAVMHASALIERELMQIAPPELADGTGTTAAATGAAL
                     PALELETEPDGHRSRLAWTVVGGEQGFGRVELLHRDGRLEALVWPDREARGEAVRLPL
                     LDGVDALRWRLIAAGQPHTQWPIAESRSRRRGRVALVTPLPEAVELTLELADVGPLTR
                     VFALR"
     gene            complement(9498..9908)
                     /gene="gspI"
                     /locus_tag="PKN25_10490"
     CDS             complement(9498..9908)
                     /gene="gspI"
                     /locus_tag="PKN25_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HNV91369.1"
                     /translation="MTPAPNRNRARPRNAGRTRGFTLLETLVALAIVAIALVSALRAM
                     GATAQSTAALREHTLASWVAQNRLAMHRATAAWPEIGEYSGEAEQARMRMRWEEKVSG
                     TPNALFRRIDVRVLDASGGRTLATLSGFAVRPLR"
     gene            complement(9905..10474)
                     /locus_tag="PKN25_10495"
     CDS             complement(9905..10474)
                     /locus_tag="PKN25_10495"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV91370.1"
                     /translation="MQRGFTLVELLVVLVIVGIAGGGIALALDRAQADDGEHEVERLR
                     RALEGAAYRAELRGRALAVDFLPDGYRFSERQPDGGWKRLEAPPEFAPRAFAGGLRVA
                     ALRVGQRAGRQALLADSGATSLPGAVAPPAGESLAAPVAEGGGGRRIVFGTRAPAFEL
                     ELAEEGMRYRIAGRIDGRVERRPGAEAAR"
     gene            complement(10503..10973)
                     /gene="gspG"
                     /locus_tag="PKN25_10500"
     CDS             complement(10503..10973)
                     /gene="gspG"
                     /locus_tag="PKN25_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNV91371.1"
                     /translation="MLQRFARPAGPAIRTHPGRRRARGFTLIEVMVVIVILGVLAALV
                     VPRIMSRPDEARVVAVRQDIASLMQALKLYKLDNRRYPSTEQGLGALVARPTAAPAPE
                     SWKAYVERLPADPWGQPYQYLAPGVHGEVDVFSYGADGRPGGEGFDADVGSWQP"
     gene            complement(10978..12210)
                     /gene="gspF"
                     /locus_tag="PKN25_10505"
     CDS             complement(10978..12210)
                     /gene="gspF"
                     /locus_tag="PKN25_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HNV91372.1"
                     /translation="MSAFRYRAFDLQGTPSTGVIEADSGRAARSALRERGLHPVEVID
                     LGQQARAAADRPGWLARVQARRRLPVNELALLSRQWATLLAAGLTVEHALSALIEQAE
                     SERVHDTLAAIRSDVVSGHSLRIALDRHSGAFPPIYRAAIAAGEQSGELAAVMNQLAD
                     HLERGADLQRKTMQALIYPALVAMVALLVVTGLMVFVVPQVVGVFAQSKQALPLLTRI
                     MIGVSNFIRDWGWLALAAGAAALLGALALLRDERIRHAFDRRLLALPVLGRHLRSLDA
                     TRFASTLAILVGSGVPLLAALDAAGRVVQRLPIRAAVREAAEQVREGRPLARALAAGR
                     AFPPMLIHMVANGEATGRIDQLLERAARLQQQELETRTATLTSLLEPLLLLVMGAVVL
                     LIVLAVMQPIIEINTLMR"
     gene            complement(12221..>12748)
                     /locus_tag="PKN25_10510"
     CDS             complement(12221..>12748)
                     /locus_tag="PKN25_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV91373.1"
                     /translation="RALRAILRQDPDVIMIGEIRDLETAQIAIQASLTGHLVLATLHT
                     NDSAAALTRLVDMGIEPFLLASTLRGVLAQRLVRKLCPDCRQPAAPASTTRGWEAGQC
                     AHCGHSGYRGRAGIYELLVVDEGLRRLIHDGADEDRLRAHARAAGMHSLHEDGLRHVA
                     SGLTSAAELQRVIRA"
BASE COUNT         1825 a         4416 c         4689 g         1818 t
ORIGIN      
        1 agatcgccga gttcggccag aaggccaagg acggcaagct ctcgctcgac gacctctccg
       61 gcggcacctt ctcgatctcc aacggcggcg tgttcggctc gatgatgtcg accccgatca
      121 tcaacccgcc gcagtcggcc atcctcggca tccacgccac caaggatcgt gccgtggtcg
      181 agaacggcca ggtcgtggtt cgtccgatca actatctggc catgtcctac gaccaccgca
      241 tcatcgacgg tcgcgaggcg gtgctgggcc tggtgacgat gaaggaagcg ctggaagatc
      301 cggctcgcct gatcctggat gtgtgagcgg acgtaaggtg cgaggcgcga ggcgcaagga
      361 gcggtgagcg ctccatgcct cgcggttcgc cttgcctggg cgcccgcagg gcgcctgttc
      421 gcacccgact cctgactctc aactcctcac gggtaaccca atgtccaaag aattcgacgt
      481 cctcgtcatc ggcggcggcc ccggcggcta cgtcgccgcg atccgtgccg cgcagctcgg
      541 cttcaagacc gcctgctgcg aatccaatcc ctacgccgac ccgaagggcg agccgcgcct
      601 cggcggcacc tgcctgaacg tcggctgcat tccctccaag gcgctgctgc acacctcgca
      661 cctgttcgag gaagccggcc acgccttcga aggccagggc atcagcgtcg gcacgcccaa
      721 gatcgacgtg cccaagatga tcgcccgcaa gtcgggcatc gtcgaccagc tcaccggcgg
      781 catcaagggc ctgttcaaga agaacaaggt caccctgctc aacggccatg gcgccttcgt
      841 cggcaagggc gacgccggct ggcaggtcca ggtcggcgaa gagctcgtca ccgccaagca
      901 ggtcatcgtc gccaccggct cggccccgcg ccacctgccg ggcgtgccgg tcgacaacaa
      961 gatcgtctgc gacaacgtcg gcgcgctcga cctcgacgcg gtgccgaaga agctcgccgt
     1021 catcggcgcc ggcgtcatcg gcctcgagat gggctcggtg tggcgtcgcc tcggtgccga
     1081 ggtcaccgtg ctcgaagcca tgcccgagtt cctctcgctc gccgaccagg acgtggccaa
     1141 ggaagcgctc aaggtcttca ccaagcaggg cctcaagttc aacctcggcg tcaccatcgg
     1201 tgaggtcaag gtgggcaaga agggcgtcag catcgcctac agggacaagg acggcgccga
     1261 gcagaagctc gaagccgacc gcctgatcgt ctccgtcggc cgcgtgccca acacccaggg
     1321 cctcaacgcc gaagccgtcg ggctcaagct caacgagcgc ggccagatcg aggtggacga
     1381 ccactgcaag accaacctgc ccggcgtgtg ggcggtgggc gacgtggtgc gcggcccgat
     1441 gctcgcgcac aaggcgatgg aagaggcggt catggtcgcc gagctgatgg cgggccaggc
     1501 cggccactgc aacttcgaca ccgtgccctg ggtgctgtac accagccccg agatcgcctg
     1561 ggtcggcaag accgagcagc agttgaaggc cgccggcgtg gcctacaagg tcggcaagat
     1621 ccccttcctg gccaacggcc gcgcgctcgg catgggcgac tccaccggct tcgtcaagat
     1681 gctggccgac gccactaccg accgcatcct cggcgtgcac atcatcggcg ccaacgcctc
     1741 ggagctgatc tccgaagccg tggtggcgat ggagttcgcc ggctgctcgg aagacctggc
     1801 gcgcatctgc cacgcccacc cgaccctgtc ggaagtggtg cacgaggccg cgctcgcctg
     1861 cgacaagcgt ccgctgcact tctgagcgag gcgtgagctg tgaggggtga ggtgcggggg
     1921 cttccctgcg cccacccctt tttgcgttaa cctgcagtcc tccgatcaac gctcctgacc
     1981 gatttcctat gcctcaccgc atcctgaacg ttccccagca cgggatgatc gacgcctacg
     2041 aagccctgct gaagacgcgc ggctaccagt ccgatcccgc ccagcgcgcc gcagcacagc
     2101 gcctgcaggg cctgtacacc gagctcgtcg gcttcaaggc ggcgcgccag ggcagccgca
     2161 tcaagcagtt cctgaacaag cccaagatgc cgcgcagcgt ctacttctgg ggtggcgtgg
     2221 ggcgcggcaa gagcttcctg atggactgct tctacgactc ggtgccgtat cgccgcaagc
     2281 gccgggtgca cttccacgcc ttcatgcagg aggtgcagaa cgacctgcgc aagttcaaca
     2341 acgcacccga tccgctgcag aaggtcgccg accgcatctc cgagcacacc cggctgctgt
     2401 gcttcgacga gttccatgtc tccgacatcg ccgacgcgat gatcctgggg cggctcctcg
     2461 acgcgctctt cgaccgcggc gtgatcttcg tgatgacctc gaactatccg cccgacggcc
     2521 tctatcccaa cggcctgatg cgcatcaact tcctgcccac gatcgagaag atcaagcagc
     2581 gcttcgacgt catcgaggtg gatcacggca ccgactatcg cctgcgcacg ctcgagaaga
     2641 tggagatcta cctcgtgccg cacgacgacg cggccaagcg caagatggcc gaggacttcc
     2701 gccgcctcgc cgcgagcgag ggccgcggcg gcagcgtcga gatcctcggg cgcaacatcg
     2761 acgtggtgcg ccaggccccg ggggtgatct ggttcgactt cgccagcctg tgcggcggcc
     2821 cgcgctcgca gaacgactac ctcgagatcg cgcgcgagca ccacacggtg atcctgtcga
     2881 acgtgcccaa gatgctggtc ggcaacgcct ccgaggcacg ccgcttcacc tggctgatcg
     2941 acgtgctcta cgaccaccgc gtcaagctca tcatgagcgc ggcggtcgag gcccacgatc
     3001 tctacaccga gggccacaac gcccacgagt tcgtgcgcac ggtgagccgc ctgatcgaga
     3061 tgcgcacccg cgactacctc gccgagccgc accgcaccga atagtcgggg cgcggcgccc
     3121 gtccgccgcg atgggcgtcg cttgggcgcg cgtccggccc ttggacaacc agggcggcgc
     3181 gggcgtgtcc cccgagcgct cgcgccgccc tgttcgtccc gccgtgggat caggagccga
     3241 ccacgccccc gtcatccttc gtgatcacga cggtcgccga gcgtgggcga ccgccgccgg
     3301 cctcgggcca ctgactgaac gcgagcgggt tctgcgcaag atactcgtcc atcgacaggc
     3361 tcccgcgcat gctcgcgtcc ggtgcgcgcg gatgcgaacc ggcctcgccg ggatgttgca
     3421 cgttgatgaa catggtccgg tagtcggggg agaatgcaag gccggtgatc tcgcagcccg
     3481 aaggcccgac caggaagcgg cggatctcct tgctctcggg gtcggcaacc agcatctggt
     3541 tgttgccctg gcccgcgtag tcgccgctat tcgagaagtt tccgtcggtc tggatccaca
     3601 gccggcccgc gccgtcgaag ccgatgccgt cggggctgtt gaaggtgttg tcggcggata
     3661 cgttcgcgga accggagcgc aggtcctggc ggtcggggta agccaccggg ttgcctgcga
     3721 gcacgaagag atcccactcg aaagtcatcg cggcggcgtc gtcgccggcc tcgcgccagc
     3781 gcacgatctg tccgtagcgg ttctgcgcgc gcgggttcgc ctcgtccgtc gtggtcctgc
     3841 cggagttgtt ggtcagggca caatagacct cgccggtttg cggatgcgtg gtgatccact
     3901 ccgggcgatc catcttcgtc gcaccgacgg cgtcggcggc gaggcgggcg tggatcagga
     3961 cttcggcctg ggtggcgaag cgggcgtctg ccgccagcgt ggggttggcc gccttgtcga
     4021 gcagcagcca ttcccccaca cccatgaagt cgccgctggc cgcgccggcg tcgaatttgg
     4081 ccacatacag cttgccggcg tcgagcaggt tgccgttcgc aagcccacgg cttgcgtcga
     4141 aaacgccatc ggacacgaac ttgtagatgt aatcgttggc ctggtcgtcg cccatgtaga
     4201 cgaccacgcg cttgtctgcg gcgagcctca tctcggcgtt ttcatgcttg aagcggccga
     4261 gcgcggtgcg cttcttcggc gtggagccgg gctcgaaggg attgatctcg acgacccagc
     4321 cgaagcggtt ggcctcgttg ggttccttgg cgtagtcgaa gcggtcctca tgctcgtgcc
     4381 agcggtagcc ggaaccgccg gcgctgatgc cgtagcggac gaaggccgcg ctgcgcgtcg
     4441 gtgccgggct cgctgcggtg gcgaaatagc cgttgaagtt ctcttcgcag gtcaggtagg
     4501 tgttccacag cgtcttgccg ttggcgcagt tgttcagcgt tcccaagacg ttgaggccac
     4561 tggggtccgc cgtggtgcgc aggagggcgt cgccggcagc ggggccggtc agcgtcatcg
     4621 gcgtcttgcc ggtgatccgg cggttgtacg gcgagccgat gtggacttcc cagcgccccg
     4681 cgccgttctt cttgacgtgg aggaccgaga ccccatgcgc gttgagcgcc ttggagacct
     4741 tgtccgccgt ccacggcgtc atgaattcgg cgccgaacag atactcgtag ttgcagtact
     4801 cgtggttcat caccaggagg ccttcgttcg atccgtcgat cgggaagtag ctcatcccgt
     4861 cgtggttgtc gccgacctgg cgagcctgct cttcaccggt gttggtgccg tccggtcgcc
     4921 atgcggggga gtcgctgaac agcggcgatc cccagggagt gaagatctgc gccgaatagc
     4981 ccgcggggac gacgatcgca tcgccgctcg atgtggcgac ggcggcgaag ccgaggagcg
     5041 gccccgcggc aggcgcaggc gcagggggcg tcccggcgga cggagactcg gcgacgggcg
     5101 tgtcgtcgtc cgaggtacag gcagcgagcg agcccgacag gaaggtgacg gccgagaggc
     5161 ccaggccgct cttcaggaag ccgcggcggc tgaccgcacg ctccacgacg gactggaagt
     5221 gctcgttggc cgagaggttg gtcggaaggt cgtcgagcgt atcgggcttc ttcattgctt
     5281 catcactccg gatgcagggt cggaaaagcc acgagttttc ctgcgccgga ttacgcgggc
     5341 gtgaagggtt tcttacgctt cgacgagggc gatcccgcgc acgcggcgga gtgcggcggg
     5401 gggtgattca gaaacggatc cgcatgcgct cgccgtcgcg tgtcgggcgc agctccgcga
     5461 gcatgccttc caggcggcca agcagccggg gatcgccgcc gacatcgaga tcgacgcgcg
     5521 ggcgctgccc gcccgagagt tccaccttgc cattcacggc gagcgcgcgc ccgcgcaggg
     5581 cgctgatctc cagcgtcgtc accggcgctg cgatcgcggc gacgatgcga tggtcgccga
     5641 gcgcctggcc gggcaggatg tccacgccgc cggcgcgcca ctccgcctgc agcgtgcccg
     5701 cgcagcgtcc gccccagccg caggcgagcg cgggaacgtt cagctcgagg acgccgcgcc
     5761 aaccggcgcg cgcgatcgca tggggaactg cggcgagcgc ggtggcgggc ggcagctgca
     5821 ccgcgaggcg ctgcagggcg acgccgccag gatcgatctc cagcgtggcg ggggcgccgc
     5881 cgtcgatggt cagctgccag cgtagccgcg cacccgccag cgcggagggc tcgaacgccc
     5941 agcgcaggcg ggtgatcggg ctcagcgatg cgctgctgtc cgcgcttgcg agacgtccct
     6001 ggccgttcca cagcgtgccc tcggccagca ccaggcggaa gcgccctgcg ctggcatgat
     6061 cgagcgctgc gtcgagcagc agcgcgggcg cgcttgcgac gagcagtgcc cccacgcccg
     6121 cggcgaagat ggccgcgccg gtggcgaact tcactgtccg cccacctcga acatggcgtc
     6181 gaagcgcgcc agcccgtcgg taccggcggc ttgcaactcc agtcgcgtcg gacgcaggcg
     6241 ctgctcggcg tgcagggtgg cgagccagtc cacgagcgca ggcagggtgg cacggccgcc
     6301 gacttccacc cggtcgccgg cgatccgcgc cgcggcctcg atgccacgtg cgctggcggc
     6361 ggcgcggatc gagtcgggca gggccgcgag cgggggcgcg gtcggcgtgg ggcgctcgcg
     6421 cagcgcggcc aggcccaggc tgtcctgttc catgccgagg taggccaggc gcgccgccgg
     6481 caagctgcgt gcgagccgtt cacgttcggc ggcgagctgc tcggccgtgg agatcagggc
     6541 gccgccgagc acgactgcga cggcgagcac gagcatccgg cgctcgcgcg gggcgcatgc
     6601 ggcataccag gcgcgcaggc gcgcaatcgg cgagagggcc ggcgatcggc cggaagcgag
     6661 ggcgggggtc gaagaggcgg tcatcggcgt tcaggggcgg gcggcgagga gcaggcggct
     6721 gccctccgcg cgcgccgcga ggccacgcgc gtcgaggcgg acgaggaggt cggcgggcgg
     6781 cgcactggcc aactccagcg tgagccggcc atcctcgtag cgcagtgctt gcggttgcgg
     6841 cgcgccggct tcgagcgcgg cgtcgagcaa ggccaagagg tcgccggcgg cgagctgccc
     6901 atgcacctgc agtgcgcgtt cgtgcgcgcg gcgcagttgc atgcgcggtt cgagcagggg
     6961 ctggccgggc agggcctcgg cggccaggcg ctcgccgcgt tcgcgcagcg cgcgctcctc
     7021 gccgccatcg atcgcgacca gtgccgcctg caccagcaac cacgcgcctg ccgcgcccgc
     7081 ggcgagcagc gccggccggc gcagctgccg ccaccagccg tcgccgccat gcgccggcgc
     7141 gaactcgccg tgcagcaggt tggcggcgtc gttcgccagt cgccaccaat ccagcgtcgg
     7201 accctcgcgc agctccaccc cgatgcgcgc cgcaagcgca ggggtctcgg ggatgggtgt
     7261 gccggtggtc gagcgcactt ccagccgggt ggggcgcgcc tcggcgggcg tcgcgtcgag
     7321 ctgcagcgcg agccattctg cgagcgtgtc cgtgctgtcg gcgtcgagcg ccatgcccgc
     7381 ctgcaggccg gttcgcagca ccgcggtcgc gccgggcagc agcgccagcg tccactcgcc
     7441 ggaggcgggc ggggcgagct gcagcgcgct gcgtaccgcg tcgagcggtc gcccgagggc
     7501 cttgcaggtc gcgaccaggg tggcgagctc gctgcgcgcg acaacgagca cgccgacgcg
     7561 cgcgtttgcc gggtcgcggc cgctgacggt gagatgctgc aggtcggggt cgcgcgcgag
     7621 atggctctcg agcgcgtagc ggatcagttg cggctgctcg cgcttcggtg ccggcggcag
     7681 ctcgagctcg agccggatca cgaggctgcc ggcgaggacg agctcgtggc gatcggctgc
     7741 gggccagtgg cgcggatcgc tgcgcccgtg gtcgagcggc cggcgtgcgg cgtcgagcag
     7801 cacccagtcg gcgaccgggt cctgtggcca gcgctcgccg gcgagcagca tcagggtggt
     7861 ggtcatggcg tgtggtacca gaggatggcg ggccagttcg actcgcggtc gagcatggcc
     7921 tgcaggttca gtaaggcgtc gccgtaggcg gcgctgatgg tgacgaggaa gtagcggctg
     7981 cgtacgtcga gcacgcggcc ggcgggcagg gcagcacctt cgggcaggcg tccttcgagg
     8041 tcgccgaggt tgcggaacca ggcacgctcg cgtgctgcga ccaggttgcg cgcgacgtcc
     8101 aggctcaggc tgtcgagcac tgcggcgagc acttccgcgc ttgcggtgtt gatgttgatc
     8161 ggcgccccgg gcggcgcggc gacgacgtgg ggctcgagcg cggcgaaggt cgcggcggga
     8221 accagcccga gcaggacgag ctcgcgtgtc tcgaccagcg cgcgtccgcc acccgcgttc
     8281 gcgttcgtct cgccgccgct gcgcccgtcg ggggccgtac cgggggccgc ggcaggggcc
     8341 gtgccgagct cggcggtccc gccctgcagg tgggctgcga gcgcgcgggc cgtcgcgttc
     8401 gcgctcgcgt catccatccc caccagaccg agcaggcggg tgaagcgggc gatcgccggc
     8461 gtgtcggggc ggccgttggg cgcgaggtcg ttgaggttga agcgcccgga gaggtcgtgg
     8521 atcgccccgc ccacgctgcc gtcggcgcta tcgctgccga tcgcggtgac ggggatcgcg
     8581 gtggtccaga tctccccaaa atgatcggtg gacgtgcggc gcgcatcgtc ggcgaggatg
     8641 ttgcgcgacc agtccaccgc ggcgcgcccg agctgccagg cctgggcctg gtcgtggcgg
     8701 ccgccgagca tggccaggcc gtggccgaaa tcggcgagtg cggctgcggc gagcatggcg
     8761 gcgaagctga ccgtcagcag cgcgagcacc accgcggcgc cgcgctgcgc ggcccgccta
     8821 gcgcagcgcg aaaacgcggg tgagcgggcc gacatcggcg agctccaggg tgagttcgac
     8881 cgcctcgggc agcggcgtga cgagcgcgac gcggccgcgc cggcggcttc ggctctcggc
     8941 gatgggccac tgcgtgtgcg gctggccggc cgcgatcagg cgccagcgca gggcatcgac
     9001 gccgtcgagc agcggcagcc ggaccgcctc gccgcgcgcc tcgcggtcgg gccagacgag
     9061 ggcttcgagg cgaccatccc gatggaggag ttcgacccga ccgaagccct gctcgccgcc
     9121 gaccaccgtc cacgcgagcc ggctgcggtg gccgtccggt tcggtttcga gttcgagcgc
     9181 ggggagcgcc gcgcccgtgg cggctgcggt cgtgccggtg ccgtcggcga gctccggcgg
     9241 ggcgatctgc atcagctcgc gctcgatcag tgcgctcgcg tgcatcaccg cctgccagcg
     9301 cgcgagctcg gcgccgatgc gctctcgccc atccaccagt tggctggtgg cgcgccaggt
     9361 gagcacgccg atcacgccga gcaaggccat cgccaccatc agctcgatca gggtcaggcc
     9421 ggcgccccga cggcgcgcgg agcggcgacg agcggcgagg gcaggggggg gagtgcggta
     9481 cggctgcatc ggctcgttca gcgcagcgga cgcacggcga agccgctcag ggtggcgagg
     9541 gtgcgcccgc cgctcgcgtc gagcacgcgc acgtcgatac gccgaaacag tgcgttgggc
     9601 gtgccgctca ctttctcctc ccagcgcatc ctcatgcgcg cctgctcggc ttcgccgctg
     9661 tactcgccga tctccggcca ggccgcagtg gcacggtgca ttgccaggcg gttctgcgcg
     9721 acccagctcg ccagggtgtg ctcgcgcagg gctgcggtgg actgcgcggt cgcgcccatc
     9781 gcgcgcaatg ccgacaccag tgcgattgcc acgatcgcga gcgcgaccag ggtctcgagc
     9841 agcgtgaagc cgcgcgtgcg tccggcgttg cgagggcgag cccgattgcg attcggcgcc
     9901 ggagtcatcg tgcggcctcc gcgcccggcc gacgctccac acgaccgtcg atgcggccgg
     9961 cgatgcggta gcgcatgccc tcttcggcca gttccagctc gaaggccggc gcgcgcgtgc
    10021 cgaagacgat cctccgcccg cccccgcctt ccgcgaccgg ggcggcgagc gactcccccg
    10081 cggggggcgc gaccgcgccg gggagcgacg ttgcgcccga atccgccagc agcgcctgcc
    10141 gccccgcgcg ctggcccacg cgcagcgcgg ccacgcgcag gccgccggca aaggcacgcg
    10201 gggcgaactc cggcggtgcc tccagtcgct tccagccgcc gtcgggctgg cgctcactga
    10261 agcgatagcc gtcggggagg aaatccaccg cgagcgcgcg gccgcgcagc tcggcgcgat
    10321 aggcggcgcc ttcgagcgcg cggcgcaggc gttcgacctc gtgctcgccg tcgtcggcct
    10381 gggcgcggtc gagcgcgagc gcgatgccac cgccggcgat gccgacgatc accagcacga
    10441 ccagcagttc gacgagggtg aagccgcgct gcatgggctc gcgttgccga agggcggggc
    10501 gctcagggct gccaggagcc gacgtcggcg tcgaagcctt cgccgcccgg gcggccgtcg
    10561 gcgccgtagc tgaacacatc gacctcgccg tgcacgcccg gcgccaggta ctggtagggc
    10621 tggccccagg ggtcggcggg caggcgctcc acgtaggcct tccagctctc gggcgcgggc
    10681 gccgcggtcg ggcgcgcgac cagcgcgccc agaccctgct cggtgctcgg gtagcgtcgg
    10741 ttgtcgagct tgtagagctt gagcgcctgc atcagcgagg cgatgtcctg gcgcaccgcg
    10801 accacgcgcg cctcgtcggg ccggctcatg atgcgcggca cgaccagggc ggcgagcacg
    10861 ccgaggatca cgatcacgac catcacctcg atcagcgtga agccgcgggc gcggcggcgg
    10921 ccgggatggg tgcggatggc ggggccggca gggcgggcga agcgttgcag catggactta
    10981 cctcatcagg gtgttgattt cgatgatcgg ctgcatgacc gcgagcacga tcagcagcac
    11041 caccgccccc atcaccagca gcagcagcgg ctcgagcagg ctggtgaggg tggcggtgcg
    11101 ggtttcgagt tcctgctgct gcaggcgcgc ggcacgctcg agcagctggt cgatgcggcc
    11161 ggtggcttcg ccgttggcca ccatgtggat cagcatcggc ggaaacgcgc gcccggcggc
    11221 gagcgcgcgc gccagcggcc gcccttcgcg cacctgctcg gcggcttcgc gcaccgcggc
    11281 gcggatcggc aggcgctgca ccacgcggcc ggcggcgtcg agcgcggcga gcagcggcac
    11341 gccgctgccg accaggatgg cgagcgtgct tgcgaagcgg gtggcgtcca ggctgcgcag
    11401 gtggcggccg agcaccggca gcgcgagcag gcggcggtcg aaggcgtggc ggatgcgctc
    11461 gtcgcgcagc agcgctagcg cgccgagcag ggcggcggca cccgcagcga gcgcgagcca
    11521 gccccagtcg cggatgaagt tcgacacccc gatcatgatg cgggtgagca gcggcagggc
    11581 ctgcttgctc tgcgcgaaca cgcccaccac ctgcggcacc acgaaaacca tcagcccggt
    11641 caccaccagc agcgccacca tcgccaccag ggccgggtag atcagtgcct gcatcgtctt
    11701 cctctgcagg tcggcgccgc gttcgaggtg gtcggcgagc tggttcatca ccgcggcgag
    11761 ctccccggac tgctcgccgg cggcgatcgc cgcgcggtag atcggcggga aggcgccgct
    11821 gtggcggtcg agcgcgatgc gcaggctgtg gccggagacg acgtcggagc ggatcgccgc
    11881 cagggtgtcg tgcacgcgct cgctctcggc ctgctcgatc agcgccgaca gcgcgtgctc
    11941 gaccgtgagc ccggcggcga gcagggtcgc ccactggcgc gacagcagcg cgagctcgtt
    12001 caccggcagg cggcggcgcg cctgcacgcg tgcgagccag cccgggcgat cggcggccgc
    12061 gcgggcctgc tggccgaggt cgatgacctc gaccggatgc aggccgcgct cgcgcagcgc
    12121 gctgcgtgcg gcgcggcccg agtcggcctc gatcacgccg gtgctcggcg tgccctggag
    12181 gtcgaaggcg cgatagcgga aggcgctcat gacaaatggc tcaggcgcgg atcacgcgct
    12241 gcagctcggc ggcgctggtc agcccgctgg cgacgtggcg caggccgtcc tcgtgcaggc
    12301 tgtgcatgcc ggcggcgcgc gcgtgggcgc gcaggcggtc ctcgtcggcg ccgtcgtgga
    12361 tcaggcggcg caggccctcg tcgaccacca gcagctcgta gatgccggca cgcccgcggt
    12421 agccgctgtg gccgcagtgc gcgcactggc cggcttccca gccccgcgtc gtgctggcgg
    12481 gcgcggcggg ctggcggcag tcggggcaga gcttgcgcac caggcgctgg gcgagcacgc
    12541 cgcgcagggt ggaggcgagc aggaagggct cgatgcccat gtccaccagg cgggtgagcg
    12601 cggccgcgct gtcgttggtg tgcagggtgg cgagcacgag gtggccggtg aggctggcct
    12661 ggatggcgat ctgcgcggtc tcgaggtcgc ggatctcgcc gatcatgatc acgtccgggt
    12721 cctggcgcag gatggcgcgc agcgcgcg
//
LOCUS       DAOQBS010000088        12708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4059_length_12708_cov_8.445270, whole genome shotgun sequence.
ACCESSION   DAOQBS010000088 DAOQBS010000000
VERSION     DAOQBS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12708
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4059_length_12708_cov_8.445270"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..3545
                     /locus_tag="PKN25_10515"
     CDS             234..3545
                     /locus_tag="PKN25_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV91374.1"
                     /translation="MNVVDLLLQQASSRPEALAVRHGDEVLGYGALGRRVCSVAAGLA
                     NAGVARGDVLALCLDDPLDHLVASLAVAHRGATVVSVPRTLPDALRGRLLALTRAGRA
                     LADDGRRTGADLGGLPILAWSMLERSPPSMTPEPAREVADRPWIYVTGSGSTGRPKIM
                     PVTHRQQCLRSRLGPSWLPYGAQDVLCSMVSMHFYSAKQRCLEAMAIGAGIFLDTPGR
                     VDLRREVAEGEVTVIHAMVCHIETLLRRLPAEATGAFARLQALMVGGSTVSMALRDEI
                     RRRLTPRIHVFYGTNESHTATLTRLDEVHRTSGGVGRPFPGIRIEVVDERGQPVPAGV
                     DGRVRIASPMVIDGYLGDEEANRKAFAEGWFYPGDIGHLTTDGQLVHRGRMDDMMIVG
                     GVNLHPAEIEQCLRRHPGVADALATPLRHRVLQDVPVALVVPEAGTHPSAHELVAHVR
                     AALGRHALHDVIWVDRIPRSEQGKVQREAVEAVLERHDWAHEGGRAQGARRHWSRPRQ
                     LQTRFEIEFAPPIAPATNRLDAWMALLGEDAPLGTRPSEGCEDADTARYWLQQVLRLA
                     RLFLQLVRVPVFDAFELIDCAPVDEHRRRWRAAFLCPGPEFVSATACMEVVGIAFRIA
                     QLAQGADPGSPQDRERVFVAIGDELLQPHGKLMPAGKSTLEVLRAAYRLDIPFRPLGR
                     GVYQLGWGSRARRIERSTTDGDSVFGMRWTHDKWMASELLRAAGLPAPVNRLVMTAEE
                     ARAAARSLGYPVVVKPVDRDRGEGVSVDVGDDEGVARAYTTARECSPGKRVLVERQAP
                     GVCHRIFVAAGRLLYAVRRLPAGVYGNGRTSIGSLVDAECARRMGLPPWKRAPLCALD
                     DSALEMLARQGLGPEDVPGAGRFVALRRIETTASGGVDEEVTHALHPENVRAAIAAAR
                     LFGLEVAGVDMISPDITAPWHANGAILNEVNFAPLLGGGEISRRYVEEYLFRLVDGRG
                     RIPVEVFVGGAAAWEAAAARWRTLLQAGMAAVLSSDLRTLGGDGQPFGLAVDGLHARV
                     RALTLRAEVGALVLVVQTLELLRTGIPLDRVDRVVVIDGDIRTAGSPARQAADAEVAG
                     LIALCRAWDASGTAS"
     gene            3590..5374
                     /locus_tag="PKN25_10520"
     CDS             3590..5374
                     /locus_tag="PKN25_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNV91375.1"
                     /translation="MVELVRVGTRFTWLRGAAFGLQQGALAGELVLTDEGRAGIVEEM
                     LAAFADEASTGGERAAGASGVECLIDCILRCVGALQRRAGIPVSWRSHRALIRTVADV
                     RVFRVALPSDQPAATERALRWVVAEVNRRATGGQAGSKDGRSSQESLEELDRMLGAHA
                     IPGINPINIALAAYRAGIPVGRLLHDVLVLGQGRKARWMRSTITDRTSGIGLGIARNK
                     VHTAEVLRAAGLPVPQQVVVRSPEGAVEAARALGYPVVVKPADQEQGAGVQAGLSDDA
                     MVRIAYDAASALSRTILVERHVEGYTHRLTVFQGRVVRVMRRVAGGVVGDGVSAVATL
                     VERRLEDEIHRRRAQRAGAPLLSLDAEALDLLARRGMGPASVPAAGEYIRLRRRDNIN
                     AGGENRPCALGDVHPANLQLAVDAAMALRLDLAGVDLIIEDVSRSWLEAGGAICEVNG
                     QPQMGGNSHAGVLDAVVRELVGGDGRIPVHLVVCPDREDWHAGLSARLLQHGEVDGVS
                     SRHGLVLRGARVSMPFEDALAAAQALLCRADVGSAACLMSAPDILRLGLPSDVLDSLR
                     VADETAMSAAERGALAAIRPMIEPHLRR"
     gene            complement(5393..6115)
                     /locus_tag="PKN25_10525"
     CDS             complement(5393..6115)
                     /locus_tag="PKN25_10525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV91376.1"
                     /translation="MSGFSAEFVAAIRQAAQTHRGPSFRILISGCADYSAYAHVLAGC
                     AGLSVQPRVTALDLCATPLILSRWYARRHGGDLAAVCSDILDHRHPGGYELILTSSFL
                     GYFPPARRAELFSRYAALLAPGGRLIFTTRLRPGAEDQLVPFGPDQKEALVRTVVDSL
                     TELSGLGLDRREAAQLAAAYADLCASYPVNGEASLRRLAEAAGLLWLDCDRRRSEAAR
                     PGVTGPTVGAAEYLFVTLGRPA"
     gene            complement(6304..7281)
                     /locus_tag="PKN25_10530"
     CDS             complement(6304..7281)
                     /locus_tag="PKN25_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNV91377.1"
                     /translation="MNRRHFIGALAGLAAARARAGEGNGVRMIPERPTLLVAVAAGGG
                     PDSVARAIAGHLADRHGIHARIVNLPDANGERALADFLTLPADGSTWLIAADSLIVVN
                     PRLYPRESADPLHGLAPVAQVGSNFFFLAVGAEDPIRSMAELVLEARAAERPLNYASG
                     GIGSMHHLLMADLAARLGLRLNHVPYKGGGAAATGLARGDVRVAFAGASALPLVRAGK
                     LRLLAVAAPQRSPRFPDLPSLAEVAPGFDGTLWFGWYGHAGTPAPIRDAMRGLLQEAM
                     ASPQTSAALDRQHGGISPSFTSGPAFDALIERERERFARLISLLPEQTR"
     gene            7370..7897
                     /locus_tag="PKN25_10535"
     CDS             7370..7897
                     /locus_tag="PKN25_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HNV91378.1"
                     /translation="MTPTQDRRVRGQGPDRQARLWTLVLLALSGLGAWLVLGGAALGL
                     GSLRRLGPGAFPALAGAALLIAAALACLRPWSMSADDSSTPREGDDAGLRGVWAAVAG
                     LAGFASLAPVLGYVPAAFAAALLASAGDARWERRGKLLQAVVLAGLVAAMFGVGLELP
                     AASFRNPFAGVSWMP"
     gene            7894..9372
                     /locus_tag="PKN25_10540"
     CDS             7894..9372
                     /locus_tag="PKN25_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNV91379.1"
                     /translation="MSGLMAGFEVALSPGNLWFCLIGVALGTLVGVLPGVSSLAAVAM
                     LLPFTHALSPTAALVLLAGVYYGSEYGGAIAAILLNVPGTPASAVSCLDGHPMARAGR
                     AREALILSAGASFGGAVVGIGMMLAMAAPLAGLAFMLGPAEYFAIMIFGLVCTAAVGR
                     GRLAAGMVAMLAGVGLAMVGTDAQTGVARFTLGLTELRDGIPLVVLAMGLFGVSEVML
                     SMFGALPVVRGGIGERSALPRWKAWRRAAGPVLRGGVLGGLFGALPGTGPTLASIAAY
                     ALERRLSARPECFGRGAIAGLAAPEAANNAAAQTAFVPTLALGIPGSATMALMLGAMS
                     AHGVVPSPLLAVENPELFWGLMASFVIGNLLLLVLNVPLVGVWVRILRLPPQRLYPLV
                     LVLIAVATFGVRGSAFDVWAALAIGLVAYGMRRSGVHLAPVLIGFVLGPLVEDNFRRA
                     LAISQGDFAIFVSSPIALAALGAVFVLLAVAVCRSMRSARAA"
     gene            9517..10032
                     /locus_tag="PKN25_10545"
     CDS             9517..10032
                     /locus_tag="PKN25_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HNV91380.1"
                     /translation="MLKARAPRAIPLLLSLSLLLAPSMLRAQVAQGERFGDWVYECQA
                     LSGASKACALVQVHSFGEGDKRSVRFNISRPPQGGGVGMSVVVPLGIHLPNGIRGDVD
                     GKNGFAMTPLTCVAQGCIATVPLDAGMIEALKAGRVLNLEFYGNAGRQQVKVAGSLKG
                     ITSGLRASGLD"
     gene            10068..10940
                     /locus_tag="PKN25_10550"
     CDS             10068..10940
                     /locus_tag="PKN25_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91381.1"
                     /translation="MDELIRALGGLDRVVGVGIGGSRGLRIASEASDFDLVLFRRGGE
                     RVDAREILDALRACAGVCELRDPAGFVSGRLGTHRFEIFQKDLLAIEREIGQAREGAF
                     TWSIRQLFPHGDLSTGLISHVVHLIVCSDPDGHLERLRAAATPFPVALRAALVDTFLQ
                     QAEITVTHAAKISRRLDLQYLVGLCSGFVFFANLLVFAVNGVYPVLEKGGSLVIARLP
                     QRPAHYEQRIAQLFSCAAAGDYPAAVAMLETLLGELRFLAVEGRRALAADESVRTRPN
                     EAQRAGSGRGAGSG"
     gene            complement(10901..11794)
                     /locus_tag="PKN25_10555"
     CDS             complement(10901..11794)
                     /locus_tag="PKN25_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV91382.1"
                     /translation="MQRMNATLANPLLPPPVRIALALMAAGAMSACAPSGLEARRTEA
                     RSLAAHAGWERVAIDSGRFVLIAHRPHPVRRVDTLSVYIEGDGLAWLSRSEPSRDPTP
                     LDPVALGLALRQPSGAAVHLARPCQFTLDPERRNCSKQYWTSHRFAGEVVDSMSRAID
                     RLKADQGAQGLELVGYSGGGAIATLLAARRGDVERLITVAGNLDPAAWTRLHRITPLE
                     GSLDPVDVAPALSKLEQIHLAGAKDRELPAELAEGWLRRLRTPSAARLQVVPDFDHHC
                     CWVEAWPTLYPDPAPRPDPAR"
     gene            12013..>12708
                     /locus_tag="PKN25_10560"
     CDS             12013..>12708
                     /locus_tag="PKN25_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HNV91383.1"
                     /translation="MNKSHRIVWSDARNAYIVAGENASAKGKPSSVRKALATAIAALF
                     MGPGLAAAQSCEGQNPLTDAQTSTQCYENVDVTVAAGGSVAAAGTTAIGVQVQAAPYA
                     RNFANQGTLEADAEGYNAYAYGVLFEGRVSGSVVNASTGTVSVSATGQGDGYGDGYGV
                     LIRGDLGGSLANAGTIEVSAQNVTSSGYRYAGAYGVSIDGDVDGTLSNSGNVRAESRA
                     DSVGGSSYAYTDAE"
BASE COUNT         1740 a         4049 c         4822 g         2097 t
ORIGIN      
        1 agcaaggttg cgacaggcca tagcaggccg gggatcagga gctggatctg catcgaggtc
       61 gggtgtctga aggcggcgcg agtctaccat gcgtggcgct gcgggctctc cgccgcgacg
      121 ggtcgcctgc ggtggcggtc gcggcactcc acccggtgga atgcgcatat gatcgggcgc
      181 tcgggctgag ccgcacgcag ccttgttttc ccgctcaagg attcgcgcag gacatgaatg
      241 ttgtcgatct gcttctgcag caggcttcga gtcgcccgga ggccctcgcc gtgcgccatg
      301 gcgatgaagt gctcggctat ggggccttgg ggcggagggt gtgctcggtg gccgcgggcc
      361 tggcgaacgc cggagtggcg cgaggggacg tgctcgcgct atgtctggac gaccccctcg
      421 atcatctggt ggcgtccttg gcggtcgctc atcggggggc gacggtggtg agtgtcccgc
      481 gcaccctgcc cgatgcgttg cgtgggcgac tgttggcgct cacgcgggct ggtcgggcgc
      541 tcgccgacga cggtcgtcga actggagcgg acctcggcgg gctcccgatc ctcgcctggt
      601 ccatgctcga gagaagcccg ccgtcgatga cgcccgagcc ggcgcgcgag gtggcggatc
      661 ggccgtggat ctatgtgacg ggctccggct ccacggggcg acccaagatc atgccggtca
      721 cgcaccggca gcaatgcctg cgctcgaggc tggggccgag ctggctgccc tatggcgcgc
      781 aggacgtctt gtgctccatg gtgagcatgc atttctattc cgcgaagcag cgctgcctgg
      841 aggcgatggc gatcggggcg ggaatcttcc tggacacccc cggccgcgtg gaccttcgcc
      901 gtgaagtcgc cgagggcgag gtgacggtga tccacgccat ggtctgccac atcgagaccc
      961 tcctgcgtcg gcttccggcg gaggcgaccg gcgcgttcgc ccggctgcag gcgctgatgg
     1021 tggggggctc gacggtgtcg atggcgctgc gcgacgagat ccggcgacgc ttgacccccc
     1081 gcatccatgt cttctacggg acgaacgaga gccacaccgc gaccctgact cgactggacg
     1141 aggttcaccg gacgagcggc ggcgtggggc gtccttttcc cggcatccgc atcgaggtgg
     1201 tcgatgagcg cgggcagccg gtcccggcag gggtggacgg acgggtgcgg atcgcctcgc
     1261 cgatggtcat cgacggttat ctcggtgacg aggaggcgaa ccgcaaggcc ttcgcggagg
     1321 gctggttcta tcccggggac atcggccatc tgacgaccga cgggcagctg gtgcacagag
     1381 ggcgcatgga cgacatgatg atcgtcggtg gcgtgaatct ccatccagcc gagatcgagc
     1441 agtgcctgcg gcggcatccg ggcgtggccg atgcgctggc cacgcccctg cggcatcgcg
     1501 tgctgcagga tgtcccggtc gcgctggtgg ttcccgaagc tggaacgcat ccgagcgcgc
     1561 atgaactcgt cgcccatgtc cgcgcagcct tggggcgcca tgccctgcac gacgtgatct
     1621 gggtcgaccg catcccgcgg agcgaacaag gcaaggtgca gcgcgaagcg gtcgaggcgg
     1681 ttctggagcg ccacgattgg gcgcacgagg gcggtcgggc gcaaggggcg cgccgccact
     1741 ggtcgagacc gcgtcagttg cagacccgct tcgagatcga gttcgcaccg ccgatcgcgc
     1801 ccgccacaaa ccgtctggac gcctggatgg ccttgctggg cgaggacgcc ccgctcggga
     1861 cgaggccgtc cgaggggtgc gaagacgcag acacggcccg gtactggctg cagcaggtcc
     1921 tgcgtctggc gcggctgttc ctgcagttgg tccgcgtgcc ggtgttcgac gccttcgagc
     1981 tcatcgactg cgctccggtc gacgaacatc ggcggcggtg gcgggcggcg ttcctgtgtc
     2041 cgggacccga gttcgtctcc gctacggcgt gcatggaggt ggtcgggatc gcattccgca
     2101 tcgcacagct tgcacagggc gcggatccgg gcagcccgca ggaccgcgag cgtgtcttcg
     2161 tggccatcgg agacgagctt ctgcagcccc atggaaagct gatgcccgcg ggaaagtcga
     2221 ccctcgaggt cttgcgcgca gcctatcggc tggatattcc cttccggccc cttggaaggg
     2281 gcgtgtatca gctcggctgg ggcagcaggg cgaggcgtat cgagcgcagc acgaccgatg
     2341 gcgactcggt cttcggcatg cgctggacac acgacaaatg gatggcgtcc gaactgttgc
     2401 gtgcggccgg actgccggcg ccggtgaacc ggctcgtcat gacggcggaa gaagcgcgcg
     2461 cggcagcccg cagcctgggt tatccggtgg tcgtcaagcc cgtcgatcgg gatcgcggcg
     2521 aaggggtgag cgtggatgtg ggcgatgacg agggagtggc gcgggcttac acgacggctc
     2581 gtgaatgctc gccgggcaag cgggttctgg tggagcgaca ggcgccgggg gtctgccatc
     2641 ggatcttcgt tgctgcggga cgtctgctgt atgccgtgag gcggctcccg gccggcgtgt
     2701 acgggaatgg caggacttcg atcgggagcc tggtcgatgc cgagtgcgcg cgtcggatgg
     2761 gtttgccacc gtggaagcgc gcgccgcttt gcgcgctgga cgacagcgcg ctcgagatgc
     2821 tcgcgcgcca gggcctgggg ccggaggacg tgccgggtgc cggccggttc gtcgccttgc
     2881 gtcgcatcga gaccacggcc tcggggggcg tggatgagga agtcacccat gcgctgcatc
     2941 ccgagaacgt gcgtgccgcg attgccgccg cacgcctctt cggcctggag gttgccgggg
     3001 tggacatgat cagccccgac atcaccgcgc cgtggcatgc caacggcgcc atcctcaacg
     3061 aggtgaattt cgctcccctg ctgggcggtg gcgaaatatc gcggcgctac gtcgaggaat
     3121 acctgttccg cctggtcgat ggccgtgggc ggattcccgt cgaggtattc gtgggcgggg
     3181 cggccgcctg ggaggcggct gcagcgcgct ggcggacctt gctccaggcc ggcatggccg
     3241 cggtcctgag cagtgacctg cgtaccctgg gcggcgacgg ccaacccttc ggcctggcgg
     3301 tcgatgggct gcatgcaagg gtgcgtgcct tgacgctgcg cgccgaggtg ggcgcgctgg
     3361 tccttgtcgt gcagacgctc gagctgctgc ggacggggat cccgctggat cgggtcgacc
     3421 gcgtcgtcgt gatcgacggc gacatccgga cggccggttc gccagcgcgg caggccgcgg
     3481 atgcggaggt ggccgggctg atcgccctgt gccgcgcctg ggacgccagc gggactgcgt
     3541 cttgaaccgc cggggcgggt gaggattcca tcggacggca ggggagatca tggtcgaatt
     3601 ggttcgggtg ggcacccgct tcacatggct gcgcggagcc gccttcggtt tgcaacaggg
     3661 cgcactcgcc ggagaactcg tgttgacgga cgaggggcgt gccggcatcg tcgaggagat
     3721 gctggcggcg ttcgccgacg aagcgtcgac aggcggggag cgggcggcgg gcgcctcggg
     3781 cgtcgagtgc ctgatcgact gcatccttcg ctgcgtcggc gcgttgcagc gacgagcggg
     3841 aatcccggtg tcgtggagaa gccatcgtgc cctgatccgg accgtggcgg atgtgcgcgt
     3901 cttcagggtc gcgctgccgt cggaccagcc tgcggcgacc gagcgtgcgt tgcgttgggt
     3961 ggtggccgag gtgaatcgac gcgcgaccgg cgggcaggcc gggagcaagg acgggcgcag
     4021 ctcgcaggag tcgctggaag agctggatcg catgctcggc gcgcatgcga tccccggcat
     4081 caacccgatc aacatcgcac ttgccgctta ccgcgccggg atccctgtgg ggcgcctcct
     4141 gcatgatgta ctggtgctcg gtcaggggcg gaaggcgcgc tggatgagga gcacgatcac
     4201 cgaccggacg tccggcatcg gcctcgggat cgcccgcaac aaggtgcata ccgcagaagt
     4261 tctccgtgcc gcgggcctcc cggtgccgca gcaggtggtc gtgcgctcgc cggaaggcgc
     4321 ggtcgaggcc gcccgtgcgc tgggctaccc cgtagtggtc aagcctgcgg accaggagca
     4381 gggggcgggg gtgcaggccg ggctgtccga cgatgcgatg gtccgcatcg cgtatgacgc
     4441 ggccagcgcc ctgtcgcgga cgatcctcgt cgagcgccat gtcgagggct acacgcaccg
     4501 cctgaccgtc ttccagggca gggtggtccg cgtgatgcgc agggtggcgg ggggcgtggt
     4561 gggcgatggc gtgtccgctg tggccactct cgtggagcgg cgcctggagg acgagatcca
     4621 caggcggcgc gcgcagcgcg cgggggctcc cctgctgagc ctggacgcgg aggcgctcga
     4681 tctgctggcg cgccgaggca tggggcccgc atcggtgccc gcagccggcg agtacattcg
     4741 cttgcggcgg cgcgacaaca tcaatgccgg tggggaaaac cgcccctgtg cgctcggcga
     4801 cgtccatccg gcgaatctgc agctcgcggt ggacgccgcg atggcgttgc ggctggatct
     4861 ggccggcgtg gatctgatca tcgaggatgt ctccagatcc tggctggagg cgggcggggc
     4921 gatctgcgag gtcaacggcc aaccgcagat ggggggcaat tcccacgcgg gcgtgctcga
     4981 tgcggtggtg cgcgagctcg tcgggggcga cggacgcatc ccggtccacc tcgtcgtctg
     5041 tccggaccgc gaggactggc acgcgggcct ttcggcgcgg ctgctgcagc acggcgaggt
     5101 cgatggcgtg tcgtcgcgtc atggcctcgt cctgcgcgga gctcgggtca gcatgccgtt
     5161 cgaggacgcg ctggcggcgg cgcaggccct gttgtgccgg gccgatgtcg gctccgcggc
     5221 gtgcctgatg tcggcgcccg acatcctgcg gctgggtctc ccgagcgacg tgctcgacag
     5281 ccttcgggtc gccgacgaga cggcgatgag cgcggccgaa cgcggcgcgc tcgctgcgat
     5341 ccggcccatg atcgagccgc acctgcgccg gtgaggggcc ttgcccgcgt gctcaggcgg
     5401 gacgtcccag agtaacgaag aggtattccg ctgcgccgac tgtcggcccg gtcaccccgg
     5461 gccgggctgc ctcggagcgc cgccggtcgc agtccagcca gagcaggccc gcggcttcgg
     5521 cgagccggcg caggctcgct tcgccattga ccggatacga ggcgcaaagg tcggcgtaag
     5581 cggcggcgag ctgcgcggct tccctccggt ccagcccgag gccggaaagc tccgtcagcg
     5641 agtcgacgac ggtgcgcacg agcgcttcct tctggtccgg cccgaagggt acgagctggt
     5701 cttccgcgcc gggacggagc cgggtggtga agatcaggcg accgcccggc gcgagcagcg
     5761 ccgcgtagcg cgagaacagc tccgcgcgcc gggcgggggg aaagtagccc aggaagctgc
     5821 tggtgaggat cagctcgtag cctcccgggt ggcgatggtc caggatgtcc gagcagaccg
     5881 cagcgaggtc gccgccgtgc cggcgtgcat accagcgact caggatcagc ggggtcgcgc
     5941 agaggtcgag cgcggtcacg cgcggctgca ccgacaggcc ggcgcagccg gcaagcacat
     6001 gggcgtaggc ggaatagtcg gcgcagccgc tgatcaggat gcgaaacgag gggccgcgat
     6061 gggtctgggc tgcctgccgg atcgcggcga cgaattcggc gctgaagccg ctcatcgtct
     6121 ttcccagccc gagcaggcga aggtactgcc agaagccgtg gtagggctgg caggaatccc
     6181 cttgcgcgcc gcagcgacag gcgaagcgcg agatctcgag ggcaaaggga gccgattcga
     6241 gcacaggttc ggcgagcgga ccggtgcctg cgggcgcgag cgggcggggg gcctgtgcat
     6301 ggctcatcgc gtctgttccg gaaggaggga aatcagccgg gcgaagcgct cccgttcgcg
     6361 ctcgatcagc gcgtcgaagg ccggcccgga agtgaagctg ggggaaatgc cgccgtgctg
     6421 gcggtcgagt gcggcgctcg tttgcggact ggccattgcc tcctgcagca gtccccgcat
     6481 cgcgtcccgg atcggtgccg gcgtgcccgc atggccgtac cagccgaacc agagcgtgcc
     6541 gtcgaaaccc ggcgccacct cggcgaggct gggcagatcc ggaaaacgcg gcgagcgttg
     6601 cggcgccgcg actgcgagca gccgcagctt gcccgcccgc acgagcggga gggcggaggc
     6661 gccggcgaac gcgactcgca cgtcgccgcg cgcgagaccg gtcgcggcgg ctcctccgcc
     6721 cttgtaggga acgtggttca gccgcagtcc gaggcgtgcc gccaggtcgg ccatgaggag
     6781 atggtgcatg ctgccgatgc cgccgctcgc gtagttcagt gggcgctccg ccgcccgcgc
     6841 ctcgagtacg agctcggcca tgctccggat cggatcctcg gccccgaccg cgagaaagaa
     6901 gaaattgctg ccgacctggg cgaccggtgc aagaccgtgc agcgggtcgg cggactcgcg
     6961 cgggtagagc cggggattca ccacgatcag gctgtcggcc gcgatcagcc aggtgctgcc
     7021 gtctgccggc agggtgagaa aatccgccag ggcccgctcg ccgttggcgt ccgggaggtt
     7081 gacgatgcgt gcatggatgc cgtgccggtc ggcgagatgc ccagcgatcg cacgcgcgac
     7141 cgaatcgggg ccgccaccgg cggcgaccgc gacgaggagg gtcggccgct ccggtatcat
     7201 ccggacgccg tttccttctc cggctcgggc gcgcgctgcc gcgaggccgg cgagggcgcc
     7261 gatgaaatgt cgtcggttca tgaatcacgc tcgggaggaa gtggatgacc gcagagtcga
     7321 actctagagc catgcggccc cccgcgccac cgtcgggggc gtgccggcca tgactccgac
     7381 gcaggaccgc cgcgtgcggg ggcaggggcc ggatcgccaa gcgcgcctgt ggacgctggt
     7441 gctgcttgcg ctctcgggcc tcggtgcgtg gctcgtgctc gggggcgccg cgctgggcct
     7501 gggctccttg cgacgcctcg ggcccggggc gttccccgcg ctcgcggggg cggcgctgct
     7561 catcgccgca gccctggcgt gcttgcggcc atggtccatg tcggcggacg attcctccac
     7621 cccgcgcgag ggggacgacg cgggtctgcg cggcgtgtgg gcggcggtcg ccggactggc
     7681 gggtttcgcc tcgctcgcgc ccgtgctggg ttatgtgccg gccgccttcg cggctgcgct
     7741 gcttgcgagc gctggggatg cgcgctggga gcggcgtggg aagctcctgc aggccgtagt
     7801 gctggcgggc ctggtggcag cgatgttcgg cgtcgggctg gagctccccg cggcgagttt
     7861 caggaatcct tttgcggggg tgtcgtggat gccttgagcg gcctgatggc ggggttcgag
     7921 gtcgcgctgt cgccgggcaa tctctggttc tgcctgatcg gggtggcgct cggaaccctg
     7981 gtcggcgtgt tgcccggggt cagctcgctg gccgcggtcg caatgctgtt gccattcacg
     8041 catgccttgt cgccgaccgc ggcgctggtg ctgttggcgg gggtctatta cggctccgag
     8101 tacggcgggg cgatcgcggc gatcctgctc aacgtgccgg gaacgcccgc ttccgcggtt
     8161 tcatgtctcg acggtcaccc gatggcgcgc gcggggcgtg cccgcgaggc gctgatcctg
     8221 agtgcgggag cgtccttcgg cggcgcggtc gtgggcatcg gcatgatgct cgccatggcc
     8281 gccccgctcg ccgggctcgc gttcatgctc gggccggccg aatacttcgc gatcatgatc
     8341 ttcggactgg tctgcacggc cgccgtgggc cgcgggcgtc tggccgcggg catggtggcg
     8401 atgctcgccg gggtcggcct cgcgatggtc ggtaccgacg cccagaccgg cgtggcccgt
     8461 ttcaccctcg ggctgacgga actgcgcgac ggcattccgc tggtggtgct cgccatgggg
     8521 ctcttcggcg tttcggaggt gatgctgtcg atgttcggcg ccttgcctgt ggtgcgtggc
     8581 gggattggcg agcgcagcgc gctcccgcgc tggaaggcgt ggcgccgtgc agcagggccg
     8641 gtgctgcgcg ggggcgtgct gggcggattg tttggtgcct tgccgggcac cggacccacc
     8701 ctggcgtcga tcgccgcgta tgcgctcgag cgtcgtcttt ctgcccgccc ggagtgcttc
     8761 ggccgcggcg cgatcgccgg gctggcggcc cccgaggcgg ccaacaacgc ggcggcgcag
     8821 acggctttcg tgcccacgct ggcgctgggc atccccggca gcgcaaccat ggctctcatg
     8881 ctcggcgcga tgtcggccca tggggtggtg cccagcccgc tgctcgcggt cgagaatccg
     8941 gagctcttct gggggctgat ggcgagcttc gtgatcggca acctgctgct gctcgtgctc
     9001 aacgttccgc tggtgggggt ctgggttcgc atcctgcggc tgccgcccca gcgactctat
     9061 ccactcgtcc tcgtgctgat cgcggtggcg accttcggtg tgcgcggcag tgccttcgac
     9121 gtctgggcgg ccctcgccat cggcctggtg gcctacggga tgaggcgatc gggggtgcat
     9181 ctggcacccg tgctcatcgg cttcgtcctc gggccgctgg tcgaggacaa cttccgccgc
     9241 gcgctggcaa tttcgcaggg cgactttgcg atcttcgtct ccagcccgat cgccctcgcg
     9301 gcgctcggcg cggtcttcgt gctgctcgcc gtcgccgtct gccggtcgat gcgcagcgcg
     9361 agggccgcgt agggcgacgt gcccgctgcc cgccgccggg aagcgcgccg cacgtggcct
     9421 ggcgctggcg ggcgggctgc cggatgtgct gtacagtggc cgcgagcttc ggtaagaatg
     9481 aaaacacgct ttcaccccga ccagaggagc tgtccaatgc tcaaggcccg agccccgcgc
     9541 gcgattccgc tgctgctgtc cctgtcgctc ttgctcgccc cctccatgtt gcgcgcgcag
     9601 gtcgcgcagg gggagcgttt cggcgactgg gtctacgaat gccaggcgct ttccggtgcg
     9661 agcaaggcct gtgcgctggt gcaggtgcat tccttcggcg agggggataa gcgctcggtg
     9721 cgcttcaaca tcagccgtcc gccccagggc ggcggggtcg gcatgagtgt ggtcgtgccg
     9781 ctcggcattc acctgcccaa cggcatccgg ggcgacgtcg atggcaagaa cggcttcgcc
     9841 atgacaccgc tgacctgtgt cgcgcagggc tgcatcgcca cggtgccgct cgacgcgggc
     9901 atgatcgagg cactgaaggc cggccgcgtg ctcaacctcg agttctacgg caacgccggc
     9961 aggcagcagg tgaaggtggc cggctcgctg aagggcatca ccagcggctt gcgggcgagc
    10021 gggctggatt gagccgggca tgacagggac cggcggaggc ctcgggcatg gatgagctga
    10081 tccgcgcgct gggggggctc gatcgggtcg tcggggttgg catcggcggc tcgcgcgggc
    10141 tgcggatcgc ttccgaggct tcggatttcg atctggtgct gttccggcgt ggcggcgaac
    10201 gggtcgatgc gcgggagatc ctcgacgcgc ttcgtgcgtg cgccggggtt tgcgagctca
    10261 gggatcctgc gggattcgtg tcggggaggc tgggtacgca tcgattcgag atcttccaga
    10321 aggatctcct ggccatcgag cgcgagatcg ggcaggcgcg ggagggcgca ttcacctggt
    10381 cgatccggca gctctttccg catggcgact tgagcaccgg gctgatcagc catgtcgtcc
    10441 atctgatcgt gtgcagcgac cccgacggac acctggagcg cctgcgcgcc gccgccacgc
    10501 ctttcccggt cgcgctcagg gccgcgctcg tcgatacctt cctccagcag gccgaaatca
    10561 ccgtcacgca tgcggcgaag atctcccgtc ggctggatct tcaatacctc gtcgggctgt
    10621 gctcgggctt cgtcttcttc gccaacctcc tcgtctttgc ggtgaatggg gtgtatccgg
    10681 ttctggagaa ggggggcagc ctggtcatcg cccgcctgcc gcagcgtccg gcgcactacg
    10741 agcagcggat cgcgcagctc ttttcttgcg cggctgcggg ggactacccg gcggcggtgg
    10801 cgatgctcga gaccctgctc ggggagctcc ggttcctggc cgtcgagggc cgtcgagcgc
    10861 tcgccgccga cgagtcggtg cgcacgcgac cgaacgaggc tcagcgcgcc gggtcggggc
    10921 ggggtgccgg atccgggtag agcgtcggcc aggcctcgac ccagcagcag tgatggtcga
    10981 agtcgggaac cacttgcagg cgggccgcgc tgggggtgcg caagcggcgc agccatcctt
    11041 cggcgagctc agcgggcagt tcgcgatcct tcgcccccgc gagatggatc tgctccagct
    11101 tcgagagcgc gggcgccaca tcgaccggat ccagcgatcc ttcgagcggg gtgatccggt
    11161 gcaggcgtgt ccaggccgca gggtcgaggt tgccggcgac ggtgatcaga cgttcgacat
    11221 cgccgcggcg cgcggcgagc agcgtcgcga tcgccccccc gcccgaatac cccaccagtt
    11281 ccagcccttg cgcaccctga tccgccttga ggcgatcgat cgcgcggctc atcgaatcca
    11341 cgacctcgcc ggcaaagcga tgggaggtcc agtattgctt gctgcagttg cggcgctcgg
    11401 ggtcgagggt gaattggcag ggtcgggcca gatgtaccgc ggcccccgag ggttggcgca
    11461 gcgcgaggcc cagggcaacc ggatcgagcg gcgtggggtc gcgcgatggc tccgagcgac
    11521 tcagccaggc cagcccgtca ccctcgatat agacgctgag cgtgtccacg cgccgaacgg
    11581 gatgggggcg gtgagcgatc agcacgaagc gtccgctgtc gatcgcgaca cgctcccagc
    11641 ctgcatgcgc ggcgaggctc cgcgcttcgg tccggcgcgc ttcgagcccg gacggcgcgc
    11701 acgcgctcat ggcgccggct gccatgagcg cgagtgcgat gcgcacgggc ggaggaagga
    11761 gaggattcgc gagtgttgcg ttcatgcgtt gcatgtgagg tttatgccca tgcagggtat
    11821 gcaaccggat cttgttgcag tatgtttctg gataagagat cgtttctaca ccttggtgcc
    11881 gaagaagact tgaagctcgc gatgggggcg agaaacaagc gtgaagactg gcgccatacg
    11941 attgatccgg agaccccctg gctgggtcga tggacgtgtt tctcgtgact tctctaaggg
    12001 aggcttgacg tcatgaacaa gagtcatcgt atcgtctgga gcgacgcgcg caacgcgtac
    12061 atcgtcgctg gagagaatgc ttcggcgaag ggcaagccct cgtcggtcag gaaggcgctg
    12121 gccaccgcga tcgcagcgct tttcatgggg cccggcctcg ccgcagccca gtcctgtgag
    12181 gggcaaaatc cgctcaccga cgcgcagact tccacgcaat gctatgagaa cgtcgatgtg
    12241 accgtcgcgg ccggcgggag cgtcgcggcc gcgggaacga ccgcgatcgg ggtccaggtg
    12301 caggcggcgc cttacgcgcg caacttcgcg aaccagggca cgctggaggc cgatgccgag
    12361 ggctacaacg cctatgctta tggcgtgctg ttcgagggcc gggtttccgg tagtgtcgtc
    12421 aacgcgtcga ccggcacggt ctccgtctct gccaccggcc agggagacgg ctacggcgac
    12481 ggctacggtg tactgatcag gggggatctc ggcgggagtc tcgcaaacgc gggcacgatc
    12541 gaggtgtccg ctcagaacgt cacgagcagt ggctaccggt acgccggggc gtacggcgtc
    12601 tcgatcgatg gcgacgtgga tgggacgctg agcaactcgg gcaatgtccg tgccgagtcc
    12661 cgggcggata gtgttggagg ctcttcgtat gcctacaccg atgccgag
//
LOCUS       DAOQBS010000089        12617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4109_length_12617_cov_15.114791, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000089 DAOQBS010000000
VERSION     DAOQBS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12617
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4109_length_12617_cov_15.114791"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1173)
                     /locus_tag="PKN25_10565"
     CDS             complement(7..1173)
                     /locus_tag="PKN25_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV91384.1"
                     /translation="MSPLRRLALALASSLSLACALPATAAGVSADEIVVGTVSDLSGP
                     IAMLGVPVRDGMLMRFDEANAGGGVHGRKIRLAVEDAGYDPKRAVLAARKLVQHDQAF
                     AFIANMGTPVVMASMPIIVDAGRLHLFPFSPHRATYEPLHPLKFQNFAPYQDYMEAAT
                     RHMVRERGYQRSCLLYQDDDYGLEVMKGVEKALAGLGTELVERTSYKRGATDFSSQIA
                     RLRAARCDLVVLATVVRETVAAMSEARKIGWDVDMLVTASGYSAQTHELGGAAVEGLY
                     GVSVLPHPYAEGANSQLAAWIERYRARFNTEPNVWSVMGYTLADLFVRTAEATGRELT
                     PERFAHTLEGMAFTRDYFGSPAYRFSADDHLGNRKGRLAQIRNGRWELITDYLE"
     gene            complement(1286..1510)
                     /locus_tag="PKN25_10570"
     CDS             complement(1286..1510)
                     /locus_tag="PKN25_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91385.1"
                     /translation="MSAWSCPHDLDGICQRVRGAICSPGMRGCELEGKVRFALDELNE
                     PRKPVKARPAETRPEPKPGAAAAPRRRLPF"
     gene            complement(1605..2552)
                     /locus_tag="PKN25_10575"
     CDS             complement(1605..2552)
                     /locus_tag="PKN25_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV91386.1"
                     /translation="MPAVRAALPWLAVLLLALAGLGTDYALGKATLVATYAIAGLGVV
                     VVVGQAGQIALGQAALVALGAYAQALLVARGVPAPLALPVAAAAGALGGALASLPARR
                     LGGLYFGMSTLAFALIVEEALARWESLTHGAAGLTVGAFTLAGWRADSTLAQATVSVA
                     VLALAMLACARLFASRHGRAWRAVRDDEIAAAACGIDNDAAKMQAFVVGGALSGLAGG
                     LYAHWIGFISPEQFGLVFSFELLMLAFIGGARRLAGAAWGALVIVAIPPLISLLRDRL
                     PGDWARASGLELLLFGAVIVGVVLLRPAGLAGEAKGHDR"
     gene            complement(2576..3442)
                     /locus_tag="PKN25_10580"
     CDS             complement(2576..3442)
                     /locus_tag="PKN25_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004386118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV91387.1"
                     /translation="MLAFVQVLASGVAIGCVYGLVALSFVLIYKATETVSFMQGDLLM
                     LGAFAALGLHAFAGWPLVAAAVAAVLAVGVLGAVLERAVLRRALGQPHLVAVLLTFGL
                     GMMMRGGVASVPAAAQDMHRLPFAADTLAIGPLVLAASHLWVIAATVLLAGLLAAFFR
                     HTRTGLALRACSEDARSAALMGIPVARMHTLAWALGAALAAVAGLLLAPITFVHLNMG
                     FIALKAFPAAVLGGLTSLPGALVAGIFLGVAEALAGLALPEGTKDFVPYVLLLAALLL
                     FPQGLGGARGRA"
     gene            complement(3736..5910)
                     /locus_tag="PKN25_10585"
     CDS             complement(3736..5910)
                     /locus_tag="PKN25_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91388.1"
                     /translation="MSDETSKKGYQWRFFRSGGFDQVRIETADDLRHLGELDQKLWSV
                     LACPTSGLEFDSRTLQLLDVDNDGSIRAPEIIDATRWVCTVLKDPGVLFQGGDGLPLA
                     AIDETNAEGAKLLATASKVLAYVGKADAGEISMGDLAETEKLFAPEHQNGDGVVPAEL
                     AGDPRLAGAITRIVETYGAAEDRSGKPGVDQARVDAFFAAAQEVSDWHARAEADAATV
                     LPLGDATGAAAAVFEAVREKVEDYFTRCRLAAFDERAAAALNPADTAYVELSLQSLDA
                     ASAAVAALPLALVGPGRALPLAVGLNPAWEARIAALRDEVVTPILGAREVLTQAEWQD
                     LAGRFAAHRAWVAAMPANPVAVLPVADVRGLLADGTCAALTRLIDEDLAAETAAGQVD
                     ALERLVRYNRDLVRLLRNFVTLSDFYSRHDKAIFQAGTLYLDQRSCELCLRVGDMARH
                     ATLAPLSGTYLVYCECQRQGEAPMNIVAAMTGGDADEMMVPGRNGIFYDRQGRDWRAS
                     VVKVVEAPISVRQAFWSPYKRIGRMIGEQLQKFAAARDKAVNDQAATGVAGASAKIEA
                     APAAAAAPAAPAAPFDIAKFAGIFAAMGLAVGAIGTALAAVVSAFLGLPVWQMPLVVV
                     GAVLVISGPSMLLAWLKLRQRNLGPLLDANGWAVNTRARINIPFGGALTGVAALPPGA
                     SRALTDPYAEKKTPWKSWLFLVLLIAAVVVAWQQGYLDRFLG"
     gene            complement(6100..6741)
                     /locus_tag="PKN25_10590"
     CDS             complement(6100..6741)
                     /locus_tag="PKN25_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV91389.1"
                     /translation="MIQATIVPVTPFQQNCSILWCERTKKAAVVDPGGDLDRILAAVA
                     ELGVTVEKILITHGHIDHAGGTAKLARELGVPIEGPQEEDRFWIEGMPQQSKMFGFPD
                     VESFEPDRWLHDGDTVTVGEQTLKVIHTPGHTPGHVVFVHEGARLAIVGDVLFAGSIG
                     RTDFPRGDHQTLIRSIREKLFPLGDDITFIPGHGPTSTFGEERDSNPFVGAYG"
     gene            complement(7019..8362)
                     /gene="gdhA"
                     /locus_tag="PKN25_10595"
     CDS             complement(7019..8362)
                     /gene="gdhA"
                     /locus_tag="PKN25_10595"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNV91390.1"
                     /translation="MKYQSLEEFLAYVEQRNPGQPEFLQAVTEVMESLWPFISANKRY
                     AEHSLLDRLIEPERAISFRVSWVDDRGEVQVNRGYRIQHSSAIGPYKGGLRFHPSVNL
                     SILKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGRSPGEVMRFCQAFMTELYRHVGPD
                     TDVPAGDIGVGGREVGFMAGMMKKLSNNSACVFTGKGLSFGGSLIRPESTGYGTVYFA
                     NAMLERAGRDIKGMRVSVSGSGNVAQYAIEKLMELGAKPITCSDSSGTVIDEAGFTPE
                     KLAILMEIKNHYYGRASEYAERVGARFEPGVRPWHVPVEVALPCATQNELDGNDAATL
                     VKNGVIAVAEGANMPSTMEAAKIFEHNGVLYAPGKASNAGGVATSGLEMSQNAMRLSW
                     TREEVDARLKEIMLGIHAACLKYGEREDGTVSYVDGANVAGFVKVAEAMLAQGVI"
     gene            complement(8359..8538)
                     /locus_tag="PKN25_10600"
     CDS             complement(8359..8538)
                     /locus_tag="PKN25_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91391.1"
                     /translation="MNASNVIVGAGLTKIMHQRGELMHNIGLHCAMASGKTEAIACAR
                     AQEKPVFNQSMGPTS"
     gene            8667..9143
                     /gene="lysM"
                     /locus_tag="PKN25_10605"
     CDS             8667..9143
                     /gene="lysM"
                     /locus_tag="PKN25_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HNV91392.1"
                     /translation="MGLFAFIKEAGEKLFGTGEANAAAADPAAANARAAQAIHNYIVA
                     LKLAPADLGVSFDAAKSLVVVSGTAPDQATREKILLAAGNVAGVAEVENRLSVARAEP
                     EAQFHTVARGDTLSAIAKKYYGNANQYPAIFEANKPMLSHPDKIYPGQVLRIPPQA"
     gene            9240..9668
                     /locus_tag="PKN25_10610"
     CDS             9240..9668
                     /locus_tag="PKN25_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNV91393.1"
                     /translation="MARIRIELPERFAFSTDIPLLVSHINYGNHLDNAQLLGIVSEAR
                     VRFLKSMGYSELDIEGLGIIVADAALQYRSEAFHGETMRVEMGAADFHEHGCDLVWRM
                     AEAASGREVARGKTGIVFFDYGQRRKATVPEGFGRRFVPA"
     gene            9723..>10108
                     /locus_tag="PKN25_10615"
     CDS             9723..>10108
                     /locus_tag="PKN25_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV91394.1"
                     /translation="MAAGALAERGCTVTVYDAMPSVGRKFLLAGRGGLNLTHAEPLAD
                     FLGRYGARRDTLAPRIEAFPPQALREWAHGLGVETFVGSSGRVFPQEMKAAPLLRAWL
                     ARLRAAGVRFAVRHRWLGWAEDPAQDG"
     assembly_gap    10109..10208
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10209..11059
                     /locus_tag="PKN25_10620"
     CDS             <10209..11059
                     /locus_tag="PKN25_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNV91395.1"
                     /translation="LQHLRFATPDGEQTVEADALVLALGGGSWAKLGSDGAWVAPLRA
                     AGVEVADLLPANCGFDVVGPAGTGWSAHFLDHFRGQPLKSVAARCTDPAGRVHARAGE
                     CLISATGIEGGLVYALSAPLRDTLLARGEAVLELDLAPGRSLERLAAELARPRGSRSF
                     SSHLHSQAGIKGVKAGLLRECLAKAQFDDPARLAAAIKAMPLRLVATRPLDEAISSAG
                     GVRFEALDAQLMLRARPGVFVAGEMLDWEAPTGGYLLTACFATGLAAGTAAADWLSQQ
                     PRPPRP"
     gene            11109..11741
                     /locus_tag="PKN25_10625"
     CDS             11109..11741
                     /locus_tag="PKN25_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNV91396.1"
                     /translation="MNLHPFLVAIEVGGTLAFAMSGLIEATRKRMDIVGVFSVAFVSA
                     FGGGTLRDILLDRRPLFWIENQEYLWLVLAMVATTPLWLQTLRHKVGGRLMELADALG
                     LGLFAISGATLADAAGMPVLVSMMMGAITAVFGGVSRDVLCNEVPKVYSDHRPYAMCA
                     LFGCGVFLALDAIGLPLPIATLAGIAAATGSRLLALVFDVRLPAWPAHRD"
     gene            complement(11772..>12617)
                     /locus_tag="PKN25_10630"
     CDS             complement(11772..>12617)
                     /locus_tag="PKN25_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNV91397.1"
                     /translation="TFLLVGFVYPFFEGIAWNQAFGIQAWLKASFGAEFHDFAGSVVV
                     HAMGGWIALPAVLLLGARRGRYARNGQISAHPPSSIPFLALGAWILIVGWFGFNVMSA
                     QRLENISGLVAINSLMAMVGGTVAALAAGRNDPGFVHNGPLAGLVAVCAGSDVMHPLG
                     ALVVGAVAGGLFVVMFTLTQNRWKIDDVLGVWPLHGLCGAWGGLAAGIFGSQALGGLG
                     GVAFASQAIMTALGIAVALAGGVLVYGGLKLAVGLRLDHEEEFNGADLSIHRITATAE
                     RESNW"
BASE COUNT         1863 a         4447 c         4465 g         1742 t
ORIGIN      
        1 cgcgcctcac tccaggtagt cggtgatcag ctcccagcgt ccgttgcgga tctgggcgag
       61 gcggcccttg cggttgccga ggtggtcgtc ggcgctgaag cggtaggcgg gactgccgaa
      121 gtagtcgcgg gtgaacgcca tgccttcgag cgtgtgcgcg aagcgctcgg gggtgagctc
      181 gcgcccggtg gcctcggcgg tgcgcacgaa caggtcggcg agggtgtagc ccatcacgct
      241 ccacacgttg ggctcggtgt tgaagcgcgc gcgatagcgc tcgatccagg cggcgagctg
      301 gctgttcgcg ccctcggcgt aggggtgcgg cagcaccgag acgccgtaca ggccctcgac
      361 cgcggcgccg ccgagctcgt gggtctgcgc cgaatagccg gaggcggtga cgagcatgtc
      421 gacgtcccag ccgatcttgc gcgcttcgga catggcggcg acggtctcgc gcaccacggt
      481 ggcgagcacc acgaggtcgc agcgcgcggc gcgcaggcgc gcgatctggc tggagaaatc
      541 ggtggcgccg cgcttgtagc tggtgcgctc gacgagctcg gtgccgagcc cggccagcgc
      601 cttttcgacg cccttcatga cttccaggcc gtagtcgtcg tcctggtaga gcaggcagct
      661 gcgctggtag ccgcgctcgc gcaccatgtg gcgggtggcg gcttccatgt agtcctgata
      721 gggcgcgaag ttctggaact tgagcgggtg cagtggctcg taggtcgcac ggtggggcga
      781 gaaggggaac aggtgcaggc ggccggcatc gacgatgatc ggcatgctcg ccatgaccac
      841 cggcgtgccc atgttggcga tgaaggcgaa ggcctgatcg tgctgcacca gcttgcgcgc
      901 ggcgagcacc gcgcgcttgg ggtcgtagcc ggcgtcctcg accgcaaggc ggatcttgcg
      961 cccatgcacg ccaccgccgg cgttggcctc gtcgaagcgc atcagcatgc cgtcgcgcac
     1021 gggcacgccg agcatggcga tcgggccgga gaggtcggac acggtgccga cgacgatttc
     1081 atcggcgctc acccccgccg ccgtggcggg cagggcgcag gcgagggaca gcgaggaggc
     1141 gagcgcgagc gcaagccgac gcaaggggct catcgggaga actcctggag gttggttgga
     1201 ttgccgtgcg cggctacggc aaggcgaccg cggagcgcgc gcgagtggcc cgcaagccgg
     1261 gccggtgggt gatgcgaagc gtggatcaga acggcaggcg gcggcgcggg gctgcggcgg
     1321 cgccgggctt gggctcgggc ctggtctccg ccgggcgcgc cttgaccggc ttgcgcggct
     1381 cgttgagctc gtcgagcgcg aagcgcacct tgccctcgag ttcgcagccg cgcatgcccg
     1441 gcgagcagat cgcgccgcgc acgcgctggc agattccatc gaggtcgtgt gggcaactcc
     1501 aggcactcat ggcgggctcc ggcgcaaact gcatgcagaa ttacgagagc gttactatcg
     1561 tctgctgcag cgcgagaatc aagcgccgtc ccacctcggc ggcctcaccg gtcgtgcccc
     1621 ttcgcttcgc ccgcgagtcc ggccgggcgc agcagcacga cgccgacgat caccgcgccg
     1681 aacagcagca gctccagccc cgaggcgcgc gcccagtcgc cgggcaggcg gtcgcgcagc
     1741 agcgagatca gcggcgggat ggcgacgatc accagcgccc cccaggcggc gccggcgagg
     1801 cggcgtgcgc cgccgatgaa ggcgagcatg agcaactcga aggagaacac caggccgaac
     1861 tgctccgggc tgatgaagcc gatccagtgc gcgtagagcc cgcccgccag cccggacagc
     1921 gcgccgccga ccacgaaggc ctgcatcttc gccgcgtcgt tgtcgatgcc gcaggcggcg
     1981 gcggcgattt cgtcgtcgcg cacggcgcgc caggcgcgcc cgtggcgcga ggcgaaaagg
     2041 cgtgcgcaag ccagcatcgc gagcgcgagc accgccacgc tgaccgtggc ctgcgccagg
     2101 gtggagtccg cgcgccagcc ggcgagggtg aaggccccca ccgtcaggcc cgcggcgccg
     2161 tgggtgagcg actcccagcg tgcgagcgcc tcctcgacga tgagggcgaa ggcgagcgtg
     2221 ctcatgccga aatacagccc gcccagccgc cgcgccggca ggctggcgag cgcgccgccg
     2281 agcgcaccgg ccgccgcggc gacgggcagg gccagcggcg ccggcacgcc gcgcgcgacc
     2341 agcagcgcct gggcataggc gccgagcgcc acgagtgcgg cctggccgag ggcgatctgg
     2401 cccgcctggc ccaccaccac gaccacgccg aggccggcga tcgcgtaggt cgccaccaga
     2461 gtcgccttgc cgagcgcgta gtcggtaccc agcccggcga gcgcgagcag cagcaccgcc
     2521 agccagggca atgcggcgcg caccgccggc acgccaccgc gcaggatggg tgcgctcacg
     2581 cccgccctcg cgcgccgccg agcccctgcg ggaacagcag cagcgccgcc agcagcagca
     2641 cgtagggcac gaaatccttc gtgccttcgg gcaacgccag cccggcgagc gcctcggcga
     2701 cgccgaggaa gatgccggcc acgagcgcgc ccggcaggct ggtcagcccg cccagcaccg
     2761 cggcggggaa ggctttcagg gcgatgaagc ccatgttcag atgcacgaag gtgatcggcg
     2821 cgagcagcag cccggcgacc gcggcgagcg ccgcgccgag cgcccacgcc agggtgtgca
     2881 tgcgcgccac cgggatgccc atcagcgcgg cgctgcgcgc gtcctccgag caggcgcgca
     2941 gcgccagccc ggtgcgggtg tggcggaaga aggcggcgag caggccggcg agcagcacgg
     3001 tggcggcgat cacccacagg tggctggcgg cgagcacgag ggggccgatc gccagcgtgt
     3061 cggcggcgaa aggcaggcgg tgcatgtcct gcgccgccgc cggcaccgag gcgacgccgc
     3121 cgcgcatcat catgcccagg ccgaaggtga gcagcaccgc caccaggtgc ggctggccga
     3181 gcgcgcgccg cagcacggcg cgctccagca cggcgccgag cacgcccacc gccagcaccg
     3241 ccgccaccgc cgcggcgacg agcggccagc cggcgaaggc gtgcaggccg agcgcggcga
     3301 aggcgccgag catcagcagg tcgccctgca tgaagctgac cgtctcggtg gccttgtaga
     3361 tgagcacgaa ggagagtgcg accaggccat agacacagcc gatcgcgact ccgctggcga
     3421 ggacctggac gaatgcgagc atgggggcga agcatagccg aagcgccgcg cccgttcgcg
     3481 ccctacgggc cgcggcgcca cctccacccc cgcgatgctt gatgcaggag cgccgcaagg
     3541 cgcgaatgcg gcggtggagg cccgcacccg catcggctcc ctgaccgccg tcttcgcgcc
     3601 ttgcggcgct cccacgggga aagcggcagg cgtgtgcgcc acgaaaaagg gccggccggc
     3661 gggtgcctgg ggcattgcgc ccgcacccgc cggccggccc cgcatcggcc tgcttgccag
     3721 ccggggtgct cccgatcagc cgaggaatcg gtccagatag ccctgctgcc aggccacgac
     3781 caccgccgcg atcagcagca cgaggaagag ccagctcttc cacggcgtct tcttctcggc
     3841 atacggatcg gtgagcgcgc gcgaggcgcc cggcggcagc gccgcgacgc cggtgagcgc
     3901 gccgccgaag gggatgttga tgcgcgcacg cgtgttgacc gcccagccgt tggcgtcgag
     3961 cagcgggccg aggttgcgct ggcgcagctt gagccaggcc agcagcatcg acggaccgga
     4021 gatcaccagc accgccccca cgaccaccag cggcatctgc cacaccggca ggccgaggaa
     4081 ggccgacacc accgcggcga gtgcggtgcc gatcgcgccg acggcgagac ccatggcggc
     4141 gaagataccg gcgaacttgg cgatgtcgaa cggtgccgcc ggtgcggcgg gcgctgcggc
     4201 cgccgccggg gcggcctcga tcttggcgct ggcgccggcg acgccggtcg cggcctggtc
     4261 attgaccgcc ttgtcgcgcg cggcggcgaa cttctgcagc tgctcgccga tcatgcggcc
     4321 gatgcgcttg tacggcgacc agaaggcctg gcgcacgctg atcggcgcct ccaccacctt
     4381 gaccacgctg gcgcgccagt cgcgcccctg gcggtcgtag aagatgccgt tgcggccggg
     4441 caccatcatc tcgtcggcgt cgccgccggt catcgcggcg acgatgttca tcggcgcctc
     4501 gccctggcgc tggcactcgc aatacaccag gtaggtgccc gacagcggcg cgagcgtggc
     4561 gtggcgggcc atgtcgccca cgcgcaggca gagctcgcag ctgcgctggt cgagatagag
     4621 cgtgccggcc tggaagatcg ccttgtcgtg gcgcgagtag aagtcgctca gggtgacgaa
     4681 gttgcgcagc aggcggacga ggtcacggtt gtagcgcacc aggcgctcga gcgcgtcgac
     4741 ctggccggcc gcggtctcgg cggcgaggtc ctcgtcgatc aggcgggtga gcgcggcgca
     4801 ggtgccgtcg gcgagcagcc cccgcacgtc ggcgaccgga agcacggcca ccgggttggc
     4861 cggcatcgcc gccacccagg cgcgatgggc ggcgaagcgc ccggccaggt cctgccattc
     4921 ggcctgggtg aggacttcgc gcgcgcccag gatgggggtg acgacctcgt cgcgcagcgc
     4981 ggcgatgcgc gcctcccagg ccgggttcag cccgaccgcc agcggcagcg cgcggcccgg
     5041 gcccacaagc gcgagcggca gtgcggcgac cgcggccgag gcggcatcga gcgactgcag
     5101 cgagagctcg acataggcgg tgtcggccgg gttgagcgcg gcggcggcgc gctcgtcgaa
     5161 ggcggcgagg cggcagcggg tgaaatagtc ctcgaccttc tcgcgcacgg cctcgaagac
     5221 cgcggcagca gcgccggtgg cgtcgccgag cggcagcacg gtggcagcgt cggcctcggc
     5281 gcgggcgtgc cagtcggaga cctcctgcgc cgcggcgaag aaggcgtcca cgcgcgcctg
     5341 gtcgacgccc ggcttgccgc tgcggtcctc ggcggcgccg taggtctcga cgatgcgggt
     5401 gatcgccccg gccaggcgcg gatcgccggc gagctcggcg ggcaccacgc cgtcaccgtt
     5461 ctggtgctcg ggcgcgaaca gcttctcggt ctcggcgagg tcgcccatgg aaatctcgcc
     5521 cgcgtcggcc ttgccgacgt aggccagcac cttggaggcg gtggcgagca gcttggcgcc
     5581 ctcggcgttg gtctcgtcga tggccgccag cggcaggccg tcgccgccct ggaagagcac
     5641 gcccggatcc ttcagcaccg tgcataccca gcgcgtggcg tcgatgatct cgggcgcgcg
     5701 gatgctgccg tcgttgtcga cgtcgagcag ctgcagcgtg cgcgaatcga actccaggcc
     5761 ggaggtcgga caggccagca ccgaccacag cttctggtcg agctcgccga ggtggcgcag
     5821 gtcgtcggca gtctcgatgc ggacctggtc gaagccaccc gagcggaaga atcgccactg
     5881 gtagcctttc ttgctggttt cgtcgctcat gttctcgaac actccctgtc gttatgcggg
     5941 cagcacccgc aagccatgct cagggaaaga tggtgcgccc gaaaatcggg ccgaacaagc
     6001 gatggatttc ccgaatccgg cttaaggtcc aggggaacgg ggaggtcgaa tgacgaaacg
     6061 cgaaaaacgt cgcaggcatc gcggcgaacg ccccgcagct cagccgtagg cgccgacgaa
     6121 ggggttgctg tcgcgctcct cgccgaaggt cgaggtggga ccgtggccgg ggatgaaggt
     6181 gatgtcgtcg ccgagcggga agagcttctc gcggatcgag cggatcaggg tctggtggtc
     6241 gccgcgcggg aagtcggtgc ggccgatcga gccggcgaag agcacgtcgc cgacgatcgc
     6301 caggcgcgcg ccctcgtgca cgaagacgac gtggccgggg gtgtggccgg gggtgtggat
     6361 gaccttcagc gtctgctcgc ccacggtgac ggtgtcgccg tcgtgcagcc agcggtcggg
     6421 ctcgaaggac tcgacgtcgg ggaagccgaa catcttgctc tgctgcggca tgccctcgat
     6481 ccagaagcgg tcctcctcct gcggcccctc gatgggcacg ccgagttcgc gcgccagctt
     6541 cgcggtgccg cccgcgtggt cgatgtggcc gtgggtgatg aggatcttct cgaccgtgac
     6601 gccgagctcg gcgaccgcgg cgaggatgcg gtcgaggtcg ccgcccgggt cgaccaccgc
     6661 cgccttcttc gtgcgctcgc accacaggat gctgcagttc tgctggaacg gggtgaccgg
     6721 gacgatggtg gcctggatca tgggcgacgg actcctgttg acgggtttgc gcggcagttt
     6781 agcggatgca cgccgtcgac ccgcacgaga tgccggcagc gggcggggca ggcgcatggg
     6841 gattgccgtg caggagcgtc gagaggctgc ggttgcgtgg accgcctgcc gtcgattcgg
     6901 aaacgtcgga ttcgcgcctt gcggcgctcc tacagggggg ctgcggcggt ttccgtagga
     6961 gcgccgcaag gcgcgaaccc cacgtgccat gccggcgcgc cggcacggca cgcagggatc
     7021 agatcacgcc ttgcgccagc atggcctcgg cgaccttgac gaagccggcg acgttggcgc
     7081 cgtcgacgta gctcaccgtg ccgtcctcgc gctcgccgta cttcaggcag gcggcgtgga
     7141 tgccgagcat gatctccttg aggcgggcgt cgacctcctc gcgggtccac gacaggcgca
     7201 tcgcgttctg gctcatctcc aggcccgagg tcgccacgcc gccggcgttg gaggccttgc
     7261 ccggcgcata cagcacgccg ttgtgctcga agatcttcgc ggcttccatg gtgctgggca
     7321 tgttggcgcc ctcggcgacc gcgatcacgc cgttcttgac cagggtggcg gcgtcgttgc
     7381 cgtcgagctc gttctgggtc gcgcacggca gcgcgacttc caccggcaca tgccacggac
     7441 gcacacccgg ctcgaagcgg gcgccgacgc gttcggcgta ttcggaggcg cggccgtagt
     7501 agtggttctt gatctccatg aggatcgcca gcttctcggg ggtgaagccg gcctcgtcga
     7561 tcacggtgcc gctcgagtcg gagcaggtga tcggcttggc gccgagctcc atcagcttct
     7621 cgatggcgta ctgggcgacg ttgcccgagc ccgacaccga gacgcgcatg cccttgatgt
     7681 cgcggccggc gcgctcgagc atggcgttgg cgaagtagac cgtgccgtag ccggtcgact
     7741 cgggacggat cagcgagccg ccgaaggaca ggcccttgcc ggtgaacacg caggccgagt
     7801 tgttcgacag cttcttcatc atgccggcca tgaagccgac ctcgcggccg cccacgccga
     7861 tgtcgccggc gggcacgtcg gtgtcgggac cgacgtgacg gtacagctcg gtcatgaagg
     7921 cctggcagaa gcgcatgacc tcacccgggc tgcggccctt gggatcgaag tcggagccgc
     7981 ccttgccacc gcccatcggc agcgtggtga gcgcgttctt gaaggtctgc tcgaaggcga
     8041 ggaacttcag gatcgacagg ttcaccgagg ggtggaagcg caggccgccc ttgtaggggc
     8101 cgatcgcgga gctgtgctgg atgcggtagc cgcggttgac ctgcacctcg ccacggtcgt
     8161 ccacccaaga gacgcggaac gagatcgcgc gctcgggctc gatcagccgg tcgaggaggg
     8221 agtgctccgc atagcgcttg ttggccgaga tgaagggcca caggctctcc atgacctcgg
     8281 tcacggcctg cagaaactcg ggctggccgg ggttgcgctg ttcgacgtag gccaggaact
     8341 cctcgaggga ctgatatttc atgatgttgg acccatggat tgattgaaga caggtttttc
     8401 ttgcgcacgc gcacacgcga tggcctcggt tttacccgag gccatcgcgc agtgcaggcc
     8461 aatattgtgc atcaactccc ctctttggtg catgatcttc gtcaaacctg cgccgactat
     8521 gacatttgat gcattcatgc accgttcttg tgcgtaacga tgaatgtcat ccgaccctgc
     8581 gttcaggcgc cgtgcatgga ccgggaatga cccaggggtt atcctgaccg tgcacgtgta
     8641 gccaaaccaa cgacagggag gtgttcatgg ggctgttcgc attcatcaag gaagccggcg
     8701 agaagctgtt cgggacgggc gaggccaacg cggcggccgc cgatccggcc gcggccaatg
     8761 ccagggcggc gcaggcgatc cataactaca tcgtcgcgct caagctcgcg ccggcggacc
     8821 tgggcgtgag cttcgacgcc gcgaagagcc tggtcgtcgt cagtggcacc gcgcccgacc
     8881 aggccacgcg cgagaagatc ctgctcgccg ccggcaacgt cgctggcgtg gccgaggtcg
     8941 agaaccgcct gtcggtggcg cgtgccgaac ccgaggcgca gttccacacc gtggcgcgcg
     9001 gcgacacgct gtcggcgatc gccaagaagt actacggcaa cgccaaccag tacccggcga
     9061 tcttcgaggc caacaagccg atgctgagcc acccggacaa gatctatcct ggccaggtgc
     9121 tgcgcatccc gccgcaggcc tgatcacgca tcaccggcag gcggctttgc gcttgccccg
     9181 ccgaggcccg ggccgttacg ctcgggcctc ttccgtttcc gcctccgcaa acatccacca
     9241 tggcccgcat ccggatcgag ctgcccgagc gcttcgcctt ctccaccgac atcccgctgc
     9301 tggtgtcgca catcaactac ggcaaccacc tcgacaacgc ccaactgctc ggcatcgtct
     9361 ccgaggcgcg cgtgcgcttc ctcaagtcca tgggctattc cgagctcgac atcgagggcc
     9421 tcggcatcat cgtcgccgac gccgcgctgc aataccgctc cgaggccttc cacggcgaga
     9481 ccatgcgggt ggagatgggc gcggccgact tccatgagca cggctgcgac ctggtgtggc
     9541 gcatggcgga ggcggccagc ggacgcgagg tcgcgcgcgg caagaccggc atcgtcttct
     9601 tcgactacgg ccaacgccgc aaggcgacgg tgccggaagg tttcggccgc cgcttcgttc
     9661 cggcctaggc acccgtggag cgcgcacgcg tcgcggtcgt cggtggcggc cccgccggcc
     9721 tgatggcggc gggcgcgctc gccgagcgcg gctgcacggt cacggtgtac gacgccatgc
     9781 cctcggtcgg gcgcaagttc ctgctcgccg ggcgcggcgg gctcaacctc acccacgccg
     9841 agcccctggc ggacttcctc ggccgctacg gcgcccgccg cgacacgctc gcgccgcgca
     9901 tcgaggcctt tccgccgcag gcgctgcgcg aatgggcgca cggcctgggt gtcgagacct
     9961 tcgtcggcag ctccggccgc gtcttcccgc aggagatgaa ggccgcgccg ctgctgcgcg
    10021 cctggctcgc ccgcctgcgc gcggccggcg tgcgcttcgc ggtgcgtcac cgctggctgg
    10081 gctgggcgga ggatcccgcg caggatggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnngc ctgcagcacc tgcgcttcgc caccccggac ggcgagcaga cggtcgaggc
    10261 cgacgcgctc gtgctcgcgc tcggcggcgg cagctgggcg aagctcggtt ccgatggcgc
    10321 ctgggtggca ccgctgcgcg ccgccggggt cgaggtcgcc gacctgctcc cggccaactg
    10381 cggtttcgac gtcgtcggcc ccgccggcac gggctggagc gcgcacttcc tcgatcactt
    10441 ccgcggccag ccgctcaagt ccgtcgccgc acgctgcacc gaccccgccg gccgcgtcca
    10501 tgcgcgcgcc ggcgaatgcc tgatctccgc caccggcatc gaaggcggcc tggtctatgc
    10561 gctctccgcg ccgctgcgcg acaccctgct cgcgcgcggc gaggccgtgc tcgagctcga
    10621 cctcgccccc ggccgcagcc tcgagcgcct cgccgccgag ctcgcccgcc cgcgcggcag
    10681 ccgctcgttt tccagccacc tgcacagcca ggccggcatc aagggcgtca aggccggcct
    10741 gctgcgcgaa tgcctggcga aggcccagtt cgacgatccc gcccgcctcg ccgcggccat
    10801 caaggccatg ccgctgcgcc tcgtcgccac gcgcccgctc gacgaggcca tcagcagcgc
    10861 cggcggcgtg cgcttcgagg cgctggatgc gcagctgatg ctgcgcgccc gccccggcgt
    10921 cttcgtcgcc ggcgagatgc tcgactggga agcgcccacc ggcggctacc tgctcaccgc
    10981 ctgcttcgcc accggcctcg ccgccggaac cgccgccgcc gactggctgt cgcagcaacc
    11041 ccgaccgccc cgcccctgac gcctcactcc ttccgcctca cgcctcactc ttcacgcctc
    11101 gcaccccaat gaacctccac ccattcctgg tcgcaatcga agtcggcggc accctggcct
    11161 tcgcgatgtc cggcctcatc gaggccaccc gcaagcgcat ggacatcgtc ggcgtgttct
    11221 cggtcgcctt cgtcagcgcc ttcggcggcg gcaccctgcg cgacatcctg ctcgaccgcc
    11281 gcccgctgtt ctggatcgag aaccaggaat acctctggct ggtgctggcc atggtggcga
    11341 cgacgccgct atggctgcag accctgcgcc acaaggtcgg cggccgcctg atggagctcg
    11401 ccgacgcgct cggcctcggc ctcttcgcca tctccggcgc cactctcgcc gacgccgccg
    11461 gcatgccggt gctggtgtcg atgatgatgg gggcgatcac cgcggtgttc ggcggcgtgt
    11521 cgcgcgacgt gctgtgcaac gaggtgccca aggtctatag cgaccaccgc ccctacgcca
    11581 tgtgcgcgct gttcggctgc ggcgtcttcc tcgcgctcga cgcgatcggc ctgccgctgc
    11641 cgatcgccac cctggccggc atcgccgccg ctaccggctc ccgcctgctc gcgctcgtct
    11701 tcgacgtgcg cctgccggcc tggccggcgc atcgggactg aacgcggggt cggcgaacga
    11761 ccgcctgcca gttaccagtt cgactcgcgc tcggccgtcg cggtgatgcg gtggatcgac
    11821 aggtcggcgc cgttgaactc ttcctcgtgg tccaggcgca ggcccaccgc gagcttgagc
    11881 ccgccataga cgagcacccc gccggcgagc gcgacggcga tcccgagcgc ggtcatgatc
    11941 gcctgcgagg cgaacgccac cccgcccagc ccgcccagcg cctggctgcc gaagatgccc
    12001 gccgccagcc cgccccaggc gccgcacagg ccgtgcagcg gccacacccc gagcacgtcg
    12061 tcgatcttcc agcggttctg cgtcagcgtg aacatcacca cgaacagccc ccccgccacc
    12121 gcgccgacca ccagcgcacc gagcgggtgc atcacgtccg aacccgcgca caccgccacc
    12181 agcccggcga gcggcccgtt gtgcacgaag cccgggtcgt tgcgcccggc cgcgagcgcc
    12241 gccaccgtgc cgccgaccat cgccatcagc gagttgatcg ccaccagccc ggagatgttc
    12301 tccaggcgct gcgccgacat cacgttgaag ccgaaccagc ccacgatcag gatccacgcg
    12361 ccgagcgcga ggaaggggat cgacgagggc gggtgggccg agatctgccc gttgcgcgca
    12421 tagcgcccgc gccgcgcgcc cagcagcagc accgccggca gcgcgatcca gccgcccatc
    12481 gcatgcacca ccaccgagcc ggcgaagtcg tggaactccg cgccgaagct cgccttgagc
    12541 caggcctgga tgccgaaggc ctggttccac gcgatgccct cgaagaaggg atagacgaag
    12601 ccgacgagca ggaaggt
//
LOCUS       DAOQBS010000090        12487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4172_length_12487_cov_17.155486, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000090 DAOQBS010000000
VERSION     DAOQBS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4172_length_12487_cov_17.155486"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..5940
                     /locus_tag="PKN25_10635"
     CDS             304..5940
                     /locus_tag="PKN25_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNV91398.1"
                     /translation="MSNQPKVTIVRIPGRVRPDEPLRLASWIGGDSPLFAGVAPARSF
                     RLGATGSRGTAEDTVAEAIPEDALVEIEFEGGACFWTVGADLRKRLQEAGGASARGGS
                     GADDGAWDLPSSIAAPVTGTRGLAGKLTIRALRWMGFDPVKAGLDKIVDLAETRSVPR
                     EGVFLVGDDGIRGEPLKTPIPDGERTLILLHGTASNTRGSFSKLWLRQPKQWARLRAC
                     YGERIFALEHCTLTRSPIENALKLARLLPRSGSLRLLSHSRGGLVGELLALDPARVDR
                     AGITAVLAAAQRENPPAAEALMQQEAQLFALLRELEARPALQIERFVRVACPVIGTSL
                     AGEKLDQWFSLLVLAAIADEMRRSNSRGMITSGLASLASHGLDMLAETAAAIIGSRKR
                     ADQLPGLEAQMPGSALVRILNGNPGRGELFVVAGDAAPREQDWLAELRFCLIDRYFEQ
                     DHDLVVDTRAMLDGPDRPVRKVFFRAGPTVNHFSYFGNEDSAAAVAAALTDAVDTATL
                     FHDYAGEWPLPAETARGLFAPRALADPPLQGARGIVVLVPGLCGSELQVAGREVWASV
                     PALACGGFRKTLRIDNPAVEPGDPLAGSYLALANALRSSGYVVRMHGYDWRKSVVDSI
                     KGLGDTLSTALGDATAGKLPVHAIVHSMGGVLIRAAFAQDRGLWKRWKEQPRPEARVI
                     MLGTPNQGSHAVTLLLTKRDEFFAKLAMLDLHNSERELLEIAQHFPGVLELLPNAADE
                     NGEEIRLPATWQRYCTADGQRWPVPPEAALTQSGELWRTLLAGDPLIDDERLIYVAGT
                     AKETPRLAEIAEDGRFRLLKTADGDGRVTWASGIPAKCREGGRLYYMNAVHGDMPAHT
                     PAFPALQELLEKGETGRSALTRKPVPIQARGAVEAPGEYAPVSPAMLSHYPTRSDLLA
                     AATGATLRAPRTEREEEMRTRVRVIHGDLIFVDAPVLVGHIQGTNNLEQAEYILDRAL
                     NGRMQNRLDAGIYAGPLGSYALFPPQPEERFAAGAIVIGIGRVGELSPGSLCDGVARA
                     LVAYAITRHEERKRTHDRQAATAGKPLQLPVCALLIGAAIGELSLRDSVTAILRGHRK
                     ARERLADAKLDDRIELTQLDFVELLEDRAIQALNAARDAVTLDGELRRHFCIEDTLAK
                     NEGGRQRAYAELGEDAWTRIAISAVRIADGSGTQLLFNVHGDLARTEQLYNGIALGSI
                     ERFTRQLIDTESDNEEVGRTLFELLLPNWLKDQAPDRRRAQLIIDSAVAGIPWELLRD
                     RMEDDRSDRGGAPLSVRSGLVRQLSTGRFRQRIQRADGFHALVVGDPDLGRLGDYFPQ
                     LAGAREEAAEVAQLLKDGGFDTPPALLGGSAEQIQIALYQRDWRILHLSGHGIYRETL
                     PTIDADHSAKPADEAITATGMLIGENSVLTTAHIEQMRVVPDFVFINCCSLGRIEAER
                     AMLGENRPGNPMLAANLATQLIEMGVRGVIAAGWRVLDDAAKLFASTFYEAFLAGETF
                     GEAVLAARRATFDRFPTTNTWGAYQCYGDPSLLLRRPPSSHERRRHAARYDFVAPREV
                     IAELERLTQQSRYQAMESADDRKRREERIDALARLCERRGWSGHGDIQEAFGQLWAEF
                     GDHDRAIACYRAAQGAGDGSASMKATEQLINMLARRGSGLLAAGGPEAMAKGKALLGE
                     AEQLITGVLAVAPNAERHCLLGSIWKRKLKLTADNQLDAALKSMAQAYRNAENCALEA
                     GGRLYYPALSVLHADLLRQLLGTLTPDEGKAARQRIAAVRKEIEDPAFQARDFWDRVA
                     PLDLALSEALFDTACEENADRQANDASFSKAQEEYQALLAYSSRRERESVVGTLDALK
                     TFLERLPAQRKGAHFARARELVGRLNTLADALRKLL"
     gene            complement(5992..6672)
                     /locus_tag="PKN25_10640"
     CDS             complement(5992..6672)
                     /locus_tag="PKN25_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNV91399.1"
                     /translation="MRKVRVWDLPTRLFHWLLAILVVAAYVTAQIGGSLIEWHGRVGI
                     AITGLLAFRLVWGVIGSTYARFADFVPGPAHLWAHLRGHWSGLGHNPLGALSVLALLG
                     VMLYQAVTGLFSNDEIAFSGPLRALVSSETSDWLSGLHRQNYWAIIVLVGLHVSAVLF
                     YALFKKDNLLRPMVTGMKEVDEADAQPAQGGGALAFAIALAITAAVLYFATGSFIEPP
                     PPPPPAAW"
     gene            complement(6741..>12487)
                     /locus_tag="PKN25_10645"
     CDS             complement(6741..>12487)
                     /locus_tag="PKN25_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNV91400.1"
                     /translation="PAIAIDQTNPVRTSRSTVGTMTELADHFKLLYARAAHLHCRCCG
                     KPVRRDSAVSIAADLRARAAAAGDPRLVVCFPVTVPSSFSEEEVTGLLAAQGYTRIQE
                     RLAGVGAEGQDVLQVVQDRFRASNAEDSRLAEALEAALQRGHGRLAVYASGEGVRAEK
                     SAGTPGEAVWRYSSGLHCADCDIHYSEATPGLFSFNSPIGACETCRGFGRVIGVDFGL
                     VVPDESKTLAEGAIKPWQTESYRECQQDLEKMAKKYGVAMDIPVRDLPPEHRQWLFEG
                     DPKWKNWDSSWPRYWYGVRHFFDWLETKAYKMHIRVLLSRYRSYTECPACHGARLKPD
                     ALLWRLPTRAQASCRSGASRDHHVSSSVGAAEPPIAACAAPTGGAPTVGRGLTIGGAP
                     TVGEGPTMGAAPAVGEAAAAGGIAIAGVQGLAIHELMALPVDRIRDAIAALALPGVAD
                     EATELVLGEIRSRLTYLADVGLGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFV
                     LDEPSIGLHPRDIGRILGVMTRLRDAGNTLVVVEHDPQVMVAADELLEIGPGPGERGG
                     DIVARGTPAEIAANPDSVTGPWLAGTKRIDVDRPPRPVDAATPRLKLVGARQHNLRDL
                     TVGFPLRRLTCLTGVSGSGKSTLIQDVLFPALAKHFGEAAESPGVFDRLENVDNLRGV
                     VMVDQSPIGKSSRSNPVSYVGAWDPIRALFAALPEAKQRGYTPGTFSFNAGHGRCPTC
                     TGSGFEHVEMQFLSDVWLRCPDCDGKRYRPEVLELQWNGLSVADVLELTVHEALDVFR
                     EHPKVLAALAPLADVGLDYLRLGQPVPTLSGGEAQRLKLAGHLAEAAQKKPVRKASVV
                     DKFGDKAGDKTEPGLLFLFDEPTTGLHFEDVAKLLGAFDKLLQAGHSLIVIEHNLDVI
                     AASDWIIDLGPEGGEGGGAIVVEGPPEAVRAHASSHTGAALRDYAEALARTRAGNRGL
                     SPVSADAEAPGSRRSGASRDHGGSGRAVATESAIAACAAPPGDRSLVAAEPRVEYRPV
                     AAPAIEIRHAREHNLKNVSLQIPRDQFTVITGLSGSGKSTLAFDIVFGEGQRRYLESL
                     NAYARQFVQPASRPDVDGLFGIPPTVAIEQRTSRGGRKSTVATLTEIHPFLRLMYVKL
                     GTQFCPDCNVPVTPQSFEAIVAQLQRELRGASVEVLAPLVVNRKGLYTDLAKWARGKG
                     HAQLRVDGEYLPTAKWPRLDRYKEHNIELPTGMVVVQPESEATLREFVRQALEIGKGV
                     IKVLEIGKLGAVPITFSTLRACPSCGTGFPEPDPRLFSYNAKHGWCPKCYGTGLKTAA
                     RIEDPDALDLGDAEEVIVSDEPCPACDGARLNPVARAVRFRDLGLHQLAAQPVDRAAG
                     FFADLALNERETDIARDLVSEIRGRLAFLQHVGLGYLALDRAAPTLSGGEAQRIRLAA
                     QLGSNLRGVCYILDEPTIGLHPRDNRLLLDTLEALRDRGNTLLVVEHDEDTIRRADHV
                     IDLGPGAGVRGGRVVAEGTIADLLAAPESATGECFRHPLQHPLRPRRAVALENPAIRI
                     EGAKLHNLRDIDVRIPLARLTVVTGVSGSGKSSLARDVIHANLRARLGDPEATRARRR
                     GQAQPEAVDQPLVGCAAMPGWHQVGRVLEVDQTPIGKTPRSCPATYVGFWDAIRKLFA
                     DTFDARTRGWNASRFSFNTGAGRCPVCDGAGQTTVEMSFLPDVKTPCEACGGARFNPE
                     TLSVRWKDRTVAEVLAMPVDEAVDFFSAHPAIAHPLQLLQDVGLGYLTLGQPSPTLSG
                     GEAQRIKLVTELAKVRRRPGDAEDTGGVPLPADKHSLYVLDEPTVGLHMADVDKLIRV
                     LHRLTDAGHTVLVIEHDLDVMAEADWLIDLGPEGGEGGGRVVAEGPPEVAMATPESHT
                     GRHLREFLAQRKTE"
BASE COUNT         1925 a         4441 c         4325 g         1796 t
ORIGIN      
        1 cctaggccgg catcgagccg ccgccggcag gccggcggga tcgccacact ccccgccatc
       61 gaacatcccc gaatgccggc tgcaaatacg ccgcggattc acagcggaca acgctacccg
      121 catcgtctat atgtgaatca ggattcgtcg ccatgggtcg aggcgggtgc ttctcttcag
      181 ggactgcaca cgccgcaccc gggtatctgc ctcgacccgc ggcctgcgcg cgcgggctgc
      241 cgtggcagcg gccgacgacg agcatctgtt caacgcttga agatcggagc gggagcccat
      301 tccatgagca atcagcccaa agtcaccatc gtccgcatac cgggtcgggt gcggcccgac
      361 gaaccgcttc ggcttgcgag ctggatcggg ggtgattcgc ccttgttcgc cggtgtggcg
      421 ccggcgcgtt cctttcgcct cggcgcgacc ggcagtcgcg gcacggccga agacacggtc
      481 gccgaggcga ttcccgagga cgccctggtc gagatcgagt tcgaaggcgg cgcatgtttc
      541 tggaccgtgg gcgcggatct gcgcaagcgc ctgcaggagg cgggcggcgc cagcgcacgg
      601 ggtggaagcg gcgcggacga tggcgcctgg gatctgccgt ccagcatcgc cgcgccggtc
      661 acgggtacgc gcgggctcgc cgggaaactg accatccggg ccctgcgctg gatgggcttc
      721 gatccggtga aggcgggtct ggacaagatc gtcgacctgg ccgagacacg ctcggtgccg
      781 cgcgaagggg tgtttctcgt gggcgacgac ggcatccggg gcgaacccct gaaaacgccg
      841 attcccgatg gcgagcgcac cctcatcctg cttcacggca cggcatcgaa cacgcgcggc
      901 agcttctcca agctgtggct gcgccagccc aaacagtggg cgcgcctgcg cgcatgctac
      961 ggcgagcgca tcttcgcgct cgagcactgc accctgaccc gcagcccgat cgagaacgcg
     1021 ctcaagctcg cgcgcctgct gccacgcagc ggttcgctgc gcctcctgtc gcattcccgt
     1081 ggtggcctgg tcggcgaact gctcgcgctg gatccggctc gcgtcgatcg cgccggcatc
     1141 accgcggtgc tggctgcggc gcagcgggag aatccgcctg cggcggaggc gctgatgcag
     1201 caggaggcac agctcttcgc gctgctgcgc gaactcgagg cgcgcccggc gctgcagatc
     1261 gagcgtttcg tgcgcgtggc ctgcccggtc atcggcacct ccctcgccgg ggagaagctc
     1321 gaccagtggt tctcgctgct ggtgctggcc gcgatcgccg acgagatgcg ccgcagcaac
     1381 agccgcggca tgatcacgag cgggctcgcc tccctcgcca gccatgggct ggacatgctg
     1441 gcggaaaccg cggcggccat catcggcagc cgcaagcggg ccgaccagtt gcccggcctc
     1501 gaggcgcaga tgcccggctc cgcgctggtc cgcatcctca acggcaaccc gggacgcgga
     1561 gaactgttcg tcgtcgcggg cgacgccgcc ccccgggaac aggactggct ggccgagctg
     1621 cgcttctgcc tgatcgaccg ctacttcgag caggaccacg acctcgtggt cgacacgcgc
     1681 gccatgctgg acggccccga ccgcccggta cgcaaggtgt tcttccgcgc cggcccgacc
     1741 gtcaatcact tcagctactt cggtaacgag gacagcgcag ccgcggtcgc cgccgcgctg
     1801 accgacgccg tcgacaccgc cacgctgttc cacgactacg caggggaatg gcccctgccc
     1861 gccgagaccg cgcgcggcct cttcgccccg cgtgcgctcg ccgacccgcc gctccagggc
     1921 gcgcgaggca tcgtggtgct cgtacccggc ctgtgcggct ccgaactcca ggtcgccggc
     1981 cgcgaagtgt gggcgagcgt gccggccctg gcctgcggcg gcttccgcaa gacgctgcgc
     2041 atcgacaatc cggcggtcga gccgggcgat ccgctggccg gcagctacct cgcgctggcc
     2101 aatgcgctgc gcagcagcgg ctatgtcgta cgcatgcatg gctacgactg gcgcaagtcc
     2161 gtcgtcgact cgatcaaggg actcggcgac accctgtcca ccgcgctggg cgatgccacg
     2221 gcgggcaagc tgccggtgca cgcgatcgtc cattcgatgg gcggcgtgct gatccgcgcc
     2281 gccttcgccc aggaccgcgg gctctggaag cgctggaagg aacagccgcg ccccgaggcg
     2341 cgcgtcatca tgctcggcac gcccaaccag ggctcgcacg cggtgacgct gctgctgacc
     2401 aagcgcgacg agttcttcgc caagctggcg atgctcgacc tgcacaactc cgagcgcgag
     2461 cttctcgaga tcgcccagca cttccccggt gtgctcgagc tgctgccgaa cgccgccgat
     2521 gaaaacggcg aggagatccg cctgcccgcg acctggcagc gctactgcac cgccgacggc
     2581 caacgctggc ccgttccgcc ggaggctgcg ctcacccagt ccggagaact gtggcggacg
     2641 ctgctcgcag gcgatcccct gatcgacgac gagcgcctga tctatgtcgc cggcacggcg
     2701 aaggaaacgc cccgcctcgc cgagatcgcc gaggacggac ggttccgcct gctcaagacg
     2761 gccgacggcg atggccgcgt cacctgggca agcggaatcc ccgcgaagtg ccgggaaggc
     2821 ggacggctct actacatgaa cgcggtgcat ggcgacatgc cggcgcacac gccggccttc
     2881 ccggcattgc aggagctgct ggagaagggc gagaccgggc gctccgcgct gacgcgcaag
     2941 cccgtcccca tccaggcccg cggcgccgtc gaagcccccg gggaatatgc ccccgtcagc
     3001 ccggccatgc tttcacacta cccgacgcgc tccgacctgc tggccgcggc gaccggcgcc
     3061 acgctccgcg ccccccgcac cgagcgcgag gaggagatgc ggacgcgggt ccgggtcatt
     3121 cacggcgacc tgatcttcgt cgacgcaccg gttctggtcg gccacataca gggcaccaac
     3181 aatctcgagc aggcggaata catcctcgac cgcgccctca acggtcgcat gcagaaccgc
     3241 ctggatgccg gcatctatgc gggcccgctg ggcagctatg ccctcttccc accccagccc
     3301 gaggagcgct tcgccgccgg cgccatcgtc atcggcatcg gccgcgtcgg cgagctctcg
     3361 ccgggaagcc tgtgcgacgg cgtggcgcgc gcgctggtcg cctatgcgat cacgcggcac
     3421 gaggagcgca agcgcacaca cgaccggcag gccgccaccg ccggcaaacc gctgcagttg
     3481 ccggtgtgtg cgctgttgat cggtgcggcg atcggcgagc tgagcctgcg cgattcggtc
     3541 accgccatcc tgcgcggcca tcgcaaggcg cgcgaacgcc tcgccgacgc gaagctcgac
     3601 gaccgcatcg agttgacgca gctggatttc gtcgagctgc tggaggaccg tgcgatccag
     3661 gcgctcaacg cggcacgcga cgcggtgacg ctcgacggcg agctgcgcag gcatttctgc
     3721 atcgaggaca cgctggcgaa gaacgagggc gggcgccagc gtgcctacgc cgagctcggc
     3781 gaagatgcat ggacccggat cgcgatcagc gccgtcagga tcgccgacgg cagcggcacc
     3841 cagctgctgt tcaatgtcca tggcgacctt gcgcggaccg agcagctcta caacggcatt
     3901 gcgctcggca gcatcgagcg cttcacccgc cagctgatcg acacggaaag cgacaacgag
     3961 gaagtcggcc gcaccctgtt cgaactgctt ctgcccaact ggctgaagga ccaggcaccg
     4021 gaccgccgcc gcgcccagct catcatcgat tcggccgtcg ccggcattcc ctgggagttg
     4081 ctgcgcgatc gcatggaaga cgaccgcagc gatcgcggcg gcgccccgct gtcggtgcgc
     4141 agcggcctcg tacgccaact ctccacgggc cgcttccgcc aacgcatcca gcgcgcggac
     4201 ggctttcacg cgctggtcgt gggcgatccc gacctcgggc gtctgggcga ctactttccg
     4261 cagctggccg gtgcgcgcga agaagccgcc gaggtggccc agctgctcaa ggacggcggc
     4321 ttcgacacgc cgcccgcgct gctcggtggc agtgccgagc agatccagat cgcgctctac
     4381 cagcgcgact ggcgcatcct gcacctgtcg ggccatggca tctatcgtga aaccctgccg
     4441 acgatcgatg ccgaccacag cgccaagccg gccgacgagg ccatcactgc gaccggcatg
     4501 ctgatcggcg agaacagcgt gctcaccacc gcgcacatcg agcagatgcg ggtggtaccc
     4561 gatttcgtgt tcatcaactg ctgctccctc ggtcgcatcg aggccgagcg cgcgatgctc
     4621 ggcgagaacc ggcccggcaa tccgatgctc gccgccaacc tcgcgaccca gttgatcgag
     4681 atgggcgtcc gtggcgtcat cgccgccgga tggcgggtac tggacgacgc ggcgaagctg
     4741 ttcgccagca ccttctacga agccttcctt gccggcgaga ccttcggcga ggcggtgctc
     4801 gccgcccgcc gggccacttt cgaccgcttc cccaccacca acacctgggg cgcctaccaa
     4861 tgctacggcg accccagcct gctgctgcgg cggccgccaa gcagccacga gcgccgccgc
     4921 catgccgcac gctacgactt cgtcgcaccg cgggaggtga tcgccgaact ggagcgcctg
     4981 acccagcaga gtcgctacca ggccatggaa tccgccgacg accgcaagcg tcgcgaggag
     5041 cgcatcgacg cgctcgcgag gctgtgcgag cggcgcggat ggtcggggca tggcgacatc
     5101 caggaggcct tcgggcagct gtgggcggaa ttcggcgacc atgatcgcgc catcgcctgc
     5161 taccgggcag cccagggggc cggggacggc agcgcctcga tgaaggccac cgagcaactc
     5221 ataaacatgc tcgcgcgacg gggcagcggc ctgctcgcgg cgggcgggcc cgaggccatg
     5281 gcaaagggca aggccctgct cggcgaggcc gagcagctca tcaccggagt gctcgccgtc
     5341 gctccgaatg ccgaacgtca ctgcctgctc ggcagcatct ggaagcgcaa gctgaaactc
     5401 accgccgaca accagctgga tgcagcgctg aagagcatgg cacaggccta ccgcaacgcc
     5461 gaaaactgtg ccctcgaggc gggcgggcgg ctctactacc cggcgctcag cgtcctccat
     5521 gcggacctgc tgcgccagct gctcggcacc ctcaccccgg acgagggcaa ggcggcacgg
     5581 caacgcatcg ccgccgtgcg caaggagatc gaggacccgg cattccaggc gcgcgatttc
     5641 tgggatcgtg tcgcacccct cgacctggcc ctgagcgaag ccttgttcga caccgcctgc
     5701 gaagaaaatg cggatcgcca ggcgaatgat gcgagcttca gcaaggcgca ggaggagtac
     5761 caggccttgc tcgcctacag ctcacgccgc gaacgggagt cggtcgtggg tacgctggac
     5821 gcgctcaaga ccttcctgga acgcctgcca gcgcagcgaa aaggcgctca tttcgcccgc
     5881 gcgagggagc tggtcggtcg cctgaacacc ctggccgacg ccttgcgaaa gttgctgtga
     5941 ggcatgtctg gcgcgcaccg accaccgtcc atcggtgcgc gctgtcgccc ctcaccaggc
     6001 ggcgggcggc ggaggcggag gcggctcgat gaagctgccg gtggcgaagt agagcaccgc
     6061 agcggtgatc gccagcgcga tcgcgaaggc cagcgcgccg ccgccctgcg cgggctgggc
     6121 gtcggcttcg tcgacttcct tcatgccggt gaccatcggc cgcagcaggt tgtccttctt
     6181 gaacagcgcg tagaacagca cggccgacac gtgcaggccc accagcacga tgatcgccca
     6241 gtagttctgc cggtgcagcc cggacagcca gtcgctggtc tcgctgctca ccagcgcgcg
     6301 cagcggaccg gagaaggcga tctcgtcatt ggaaaacagc ccggtgacgg cctgatagag
     6361 catgaccccg agcagggcaa gcaccgacaa ggcgccgagc gggttgtgcc ccagcccgct
     6421 ccagtgcccg cgcaggtgcg cccacaggtg ggccgggccg ggcacgaagt cggcgaagcg
     6481 ggcgtaggtc gagccgatca ccccccacac caggcggaag gcgagcagcc cggtgatggc
     6541 gatgccgacg cggccgtgcc attcgatgag gctgccgccg atctgggcgg tgacgtaggc
     6601 ggccaccacc aggatggcga gcagccagtg aaaaagacga gtgggcagat cccagactct
     6661 gaccttgcgc atgctgtatt ccttccctga gattcagcct gactgactgc gccatccggc
     6721 acaggttccg ctgcactgca tcattccgtc ttgcgctgcg ccaggaactc gcgcagatgg
     6781 cggccggtgt gcgattccgg cgtcgccatc gcgacctcgg gcgggccttc ggccaccacc
     6841 cggccaccgc cctcgccgcc ctccgggccg aggtcgatca gccagtcggc ctcggccatc
     6901 acgtcgaggt cgtgctcgat caccagcacg gtgtggccgg catccgtcag gcggtgcagc
     6961 acccggatca gcttgtccac gtcggccatg tgcaggccca cggtgggctc gtcgagcacg
     7021 tacaggctgt gcttgtcggc gggcagcggc acgccgccgg tgtcttccgc atcgcccggg
     7081 cggcggcgca ccttggcgag ctcggtcacc agcttgatgc gctgcgcctc gccgcccgac
     7141 agcgtcgggc tgggctggcc cagggtcagg tagccgaggc cgacgtcctg cagcagctgc
     7201 agcgggtggg cgatcgcggg atgcgcggag aagaaatcca ccgcctcgtc caccggcatc
     7261 gccagcacct cggccaccgt cctgtccttc cagcgcaccg acagcgtctc ggggttgaag
     7321 cgcgcgccgc cgcaggcctc gcacggcgtc ttgacgtcgg gcaggaagct catctcgacg
     7381 gtggtctggc ccgcgccgtc gcacaccggg cagcgcccgg cgccggtgtt gaaggagaag
     7441 cgcgaggcgt tccagccgcg cgtgcgggcg tcgaaggtgt cggcgaagag cttgcggatc
     7501 gcgtcccaga agccgacgta ggtcgccggg caggagcgcg gcgtcttgcc gatcggcgtc
     7561 tggtcgacct ccagcacgcg gccgacctgg tgccagccgg gcatcgccgc gcagccgacc
     7621 aggggctggt cgaccgcctc cggctgcgcc tggccgcggc ggcgggcgcg ggtcgcctcg
     7681 gggtcgccga ggcgggcgcg caggttggcg tggatgacgt cgcgcgccag gctggacttg
     7741 cccgagccgg acacgccggt caccaccgtg aggcgggcga gcgggatgcg cacgtcgatg
     7801 tcgcgcaggt tgtgcagctt ggcgccctcg atgcggatcg ccgggttctc cagcgccacc
     7861 gcgcggcgcg ggcgcagcgg gtgctgcagc gggtggcgga agcattcgcc ggtggccgat
     7921 tcgggcgccg ccagcaggtc ggcgatcgtg ccctcggcca ccacccggcc gccgcgcacg
     7981 ccggcgccgg ggccgaggtc gatgacgtgg tcggcgcggc ggatggtgtc ctcgtcgtgc
     8041 tccaccacca gcaaggtgtt gccgcggtcg cgcagggcct cgagggtgtc gagcagcagg
     8101 cggttgtcgc gcgggtgcag gccgatcgtg ggttcgtcga ggatgtagca cacgccgcgc
     8161 aggttggagc cgagctgcgc cgccaggcgg atgcgctgcg cctcgccgcc cgacagcgtg
     8221 ggcgcggcgc ggtccagcgc caggtagccg aggcccacgt gctgcaggaa ggcgaggcgg
     8281 ccgcggatct cgctcaccag gtcgcgggcg atgtcggtct cgcgctcgtt caatgccagg
     8341 tcggcgaaga agccggccgc ccggtccacc ggctgtgccg cgagctggtg caggccgagg
     8401 tcgcggaagc gcaccgcgcg cgccaccggg ttgaggcgcg cgccgtcgca ggccgggcag
     8461 ggttcgtcgc tgacgatgac ttcttccgcg tcgccgaggt cgagcgcgtc cgggtcctcg
     8521 atgcgcgccg cggtcttcag cccggtgccg tagcacttgg ggcaccagcc gtgcttggcg
     8581 ttgtacgaga acaggcgcgg atccggctcg gggaagccgg tgccgcagct cgggcaggcg
     8641 cgcagcgtgg agaaggtgat cggcaccgca ccgagcttgc cgatctccag caccttgatc
     8701 acgcccttgc cgatctccag cgcctggcgc acgaactcgc gcagcgtggc ttcgctctcg
     8761 ggctgcacca ccaccatgcc ggtgggcagc tcgatgttgt gctccttgta gcggtccaat
     8821 cgcggccact tggcggtggg caggtattcg ccatcgacgc gcagctgcgc atggcccttg
     8881 ccgcgcgccc acttggcgag atcggtgtac aggcccttgc ggttgaccac cagcggcgcc
     8941 agcacttcga ccgaggcgcc gcgcagctcg cgctggagct gcgcgacgat ggcctcgaag
     9001 ctctgcggcg tcaccggcac gttgcagtcg gggcagaact gcgtgcccag cttcacgtac
     9061 atcaggcgca ggaaggggtg gatctcggtc agcgtcgcca ccgtgctctt gcgcccgccg
     9121 cggctggtgc gctgctcgat cgccaccgtg ggcggaatgc cgaagaggcc gtcgacgtcg
     9181 ggccggctgg ccggctgcac gaactggcgg gcgtaggcgt tgagcgattc cagatagcgg
     9241 cgctggccct cgccgaagac gatgtcgaag gccagcgtgg acttgcccga gccggacagg
     9301 ccggtgatca cggtgaactg gtcgcgcgga atctgcaggc tgacgttctt gagattgtgc
     9361 tcgcgggcgt ggcggatttc gatcgcgggg gcggcgacgg ggcggtattc cacccgcggc
     9421 tcggcggcga cgagcgaccg atctcccgga ggagcggcgc aagccgcgat tgcgctttcc
     9481 gtggcgacgg cgcggccgga accgccgtga tcgcggcttg cgccgctcct acgggaaccc
     9541 ggggcctccg catccgcaga aacaggggac agaccccgat ttcctgcacg ggtgcgagca
     9601 agcgcctcag cgtagtcgcg cagcgcggca ccggtgtgcg agctcgcgtg cgcgcgcacc
     9661 gcctccggcg ggccttcgac cacgatcgcg ccgccgcctt cgccgccctc ggggccgagg
     9721 tcgatgatcc agtcggaagc agcgatcacg tcgaggttgt gctcgatcac gatcagcgaa
     9781 tggcccgcct gcagcagctt gtcgaaggcg ccgagcagct tggcgacgtc ctcgaagtgc
     9841 aggccggtgg tgggctcgtc gaagaggaag agcaggccgg gttcggtctt gtccccagcc
     9901 ttgtccccaa acttatccac aacactggct ttgcgtacag gcttcttctg cgcggcctcg
     9961 gcgaggtggc cggcgagctt gaggcgctgc gcctcgccac ccgacagcgt cggcacgggc
    10021 tggccgaggc gcaggtaatc caggccgacg tcggcgagcg gcgcgagcgc ggcgagcacc
    10081 ttgggatgct cgcggaacac atccagcgcc tcatgcacgg tgagctccag cacgtcggcc
    10141 accgacagcc cgttccattg caattcaagc acttccggcc gataacgctt gccgtcgcag
    10201 tccgggcagc gcagccacac gtcggacagg aattgcatct cgacgtgctc gaagcccgag
    10261 ccggtgcagg tcgggcagcg gccgtggccg gcgttgaagc tgaaggtgcc gggggtatag
    10321 ccgcgctgct tcgcctccgg cagggcggcg aagagggcac ggatcgggtc ccaggcgccg
    10381 acgtagctca ccgggttgga gcgcgagctc ttgccgatcg gcgactggtc caccatgacc
    10441 acgccgcgca agttatccac attctcgaga cgatcgaaga cccccggcga ttccgccgct
    10501 tccccgaaat gcttggccag cgccgggaac agcacgtcct ggatcagcgt cgattttccc
    10561 gagccggaca cgccggtcag gcaggtcagg cgccgcagcg ggaaaccgac cgtgagatcg
    10621 cgcaggttgt gctggcgcgc gccgaccagc ttcagccgcg gtgtggcggc gtccaccggg
    10681 cgcggcggac ggtcgacgtc gatgcgcttc gtgcccgcca gccaggggcc ggtcacagaa
    10741 tcgggattcg ccgcgatctc ggccggcgtg ccgcgcgcga cgatgtcgcc gccgcgctcg
    10801 cccgggccgg ggccgatctc gagcagctcg tcggcggcga ccatcacctg cgggtcgtgc
    10861 tcgaccacca ccagcgtgtt gccggcgtcg cgcagccgcg tcatcacccc gaggatgcgg
    10921 ccgatgtcgc gcggatgcag gccgatcgag ggctcgtcga gcacgaacag ggtattcacc
    10981 agcgaggtgc cgagcgcggt ggtcaggttg atgcgctgca cctcgccgcc cgacagcgtg
    11041 cgcgactggc ggtccagcgt caggtagccg aggccgacgt cggccaggta ggtgagtcgg
    11101 ctgcggatct cgccgagcac cagctcggtg gcctcgtccg ccacgccggg cagcgcgagc
    11161 gcggcgatgg cgtcgcggat gcggtcgacc ggcagcgcca tcagctcgtg gatggcgagg
    11221 ccctgcaccc ccgcgatcgc aatccccccc gccgcggctg cctcgccgac ggcgggcgca
    11281 gcccccatgg tcggtccctc gccgacggtc ggcgcccccc cgatggtcag tccccggccg
    11341 acagtcggcg caccccctgt gggagcggcg caagccgcga tcgggggttc agcggcgcca
    11401 acactcgatg aaacgtggtg atcgcggctt gcgccgctcc tacatgaagc ctgagcgcgg
    11461 gtcggcaagc gccacaacaa ggcgtcgggt ttcaaccgcg ccccatggca cgccgggcat
    11521 tccgtgtagc tgcggtagcg cgacagcagc acgcggatgt gcatcttgta agccttggtc
    11581 tccagccagt cgaagaaatg ccgcacgccg taccagtaac gcggccacga gctgtcccag
    11641 ttcttccatt tcgggtcgcc ctcgaacagc cactggcgat gctccggcgg cagatcccgc
    11701 accgggatgt ccatcgccac gccgtacttc ttcgccatct tctcgaggtc ctgctggcac
    11761 tcgcgatagc tctcggtctg ccagggcttg atcgcgccct cggccagcgt cttcgattcg
    11821 tccggcacca ccaggccgaa atccaccccg atcacccgtc cgaagccgcg acaggtctcg
    11881 caggcgccga tcggcgagtt gaacgagaac aggccgggcg tggcctccga atagtggatg
    11941 tcgcagtcgg cgcaatgcag gccggacgaa tagcgccaca ccgcctcgcc cggcgtcccc
    12001 gccgacttct ccgccctcac cccctcgccc gaggcgtaga cggcaagccg cccgtgtccg
    12061 cgctgcagcg ccgcctccag cgcctccgcc aggcgtgaat cctcggcatt cgacgcgcgg
    12121 aagcggtcct gcaccacctg cagcacgtcc tgcccctccg cgccaacgcc cgcgaggcgt
    12181 tcctggatgc gcgtgtagcc ctgcgcggcg agcagtccgg tcacttcctc ctcgctgaag
    12241 ctcgacggca cggtcaccgg aaagcacacc accaggcgcg gatcgcccgc cgccgcggcg
    12301 cgcgcgcgca ggtcggcggc gatactcacc gccgaatccc gccgcaccgg cttgccgcag
    12361 caacgacagt gcagatgcgc ggcgcgcgcg tagagcagct tgaagtggtc ggcgagctcg
    12421 gtcatcgtgc ccaccgtcga gcgcgaggtg cgcaccgggt tggtctggtc gatcgcgatc
    12481 gccggcg
//
LOCUS       DAOQBS010000091        12443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4196_length_12443_cov_15.660478, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000091 DAOQBS010000000
VERSION     DAOQBS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12443
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4196_length_12443_cov_15.660478"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /gene="lipB"
                     /locus_tag="PKN25_10650"
     CDS             complement(<1..566)
                     /gene="lipB"
                     /locus_tag="PKN25_10650"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNV91401.1"
                     /translation="MIVKRLGLVEYAPALEAMRVFTAARGADTSDEIWLLQHPPVYTL
                     GQAGKPEHLLQNPANIPLVRIDRGGQITYHGPGQLVAYLLLDLPRRRLKVRELVHLME
                     QAIIDTLADYGLAAQRKDGAPGVYIAGDKIAALGLRVRNGCSYHGLAINVDADLAPFG
                     WINPCGYEGLKTIRMKDFGIDASVDAVG"
     gene            complement(563..847)
                     /locus_tag="PKN25_10655"
     CDS             complement(563..847)
                     /locus_tag="PKN25_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HNV91402.1"
                     /translation="MADQQDAQTRQSLIEYPCDFPIKVMGARVDGFAQAVIEVVLRHA
                     PDFDPAGAEMRPSSKGNYLAVTCTFRALSQVQVDAMYRELTAHPMVKVVL"
     gene            complement(890..2017)
                     /locus_tag="PKN25_10660"
     CDS             complement(890..2017)
                     /locus_tag="PKN25_10660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNV91403.1"
                     /translation="MRFLIAVLVSLFSLSALAQTVPPPALAAKAWALVDHATGQTLAE
                     KDADARIEPASLTKLMTAYITFSKLKEGSLKPDQVVPVSTKAWRMEGSRMFIEPNKPV
                     SVDELIRGVIVQSGNDACVALAETIAGSEEAFAALMNREAQRLGMTNTHFTNSTGLPD
                     PQLYTSARDLAVLASAIVRDFPEYYGLYSLKEFTYNGIKQPNRNRLLYMDPSVDGMKT
                     GHTSSAGYCLVSTARRGERRLVSVVLGAASDSVRAQESLKLLNFGYQFYETVKLYSAD
                     EALSKFRVWKGKLNELPVGFTKDFALTVPKEAANKIQVSLESRQPLVAPLQRGQEVGT
                     LTVAVDGKPLGQYPVVALQDVEVAGFFGRLWDAIVMFFKSL"
     gene            complement(2126..3271)
                     /locus_tag="PKN25_10665"
     CDS             complement(2126..3271)
                     /locus_tag="PKN25_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNV91404.1"
                     /translation="MTPVPPVLPAAFAARPQAARAPRALVAAGLACAVALLSACSSAP
                     KREGPVAEVPAQAGKPGRSPVRGGGYYKDDGPGDDIPDNLDAIPDAEPRDEPLHRFAN
                     RPYHVMGQSFVPATELRPFRQRGHGSWYGRRFHGNPTSSGEPYDMYAMTAAHPTLPIP
                     SYARVTNLANGRSVVVRVNDRGPFLRGRVIDLSYAAAHKLGYVNGGSAEVEVEQILPG
                     EAPLVAMARPVPPLPARANEGLMPGSGPATGMKALAPLPVAQAPLPAPVASPPRAAGS
                     AIALAPPECEEGPVCGAAGGFAPPVASASAGIFLQLGAFSSFANAEGFRDTVRSQASD
                     LAERFELFADGERFRLHAGPYDTVEAARDAAERMGNVLKLKPFVVVR"
     gene            complement(3289..4431)
                     /gene="rodA"
                     /locus_tag="PKN25_10670"
     CDS             complement(3289..4431)
                     /gene="rodA"
                     /locus_tag="PKN25_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNV91405.1"
                     /translation="MNDNRFNPLALVLGFLRPLDPLLLLLLAVLLGYAHVLMQSASPE
                     RLDSQITHIGIALAGMWLLAWLKPQRLLSMAVPLYLLGVVLLLAVELFGEISKGAQRW
                     LDLGVARIQPSELMKIAMPLMLAWFFQMREGQTRLVDFILAGVLLLVPVGLILIQPDL
                     GTSLLVAASGIYVIYFAGLSWKLIIPLVLVGVIGLGSIVAFGDTLCQPEVDWQVLREY
                     QKQRVCTLLDPTRDPLGRGFHIIQSTIAIGSGGLTGKGWMDGTQTHLAFLPERHTDFI
                     FAVLAEEFGLVGAVLLLVIYVLLLLRGFHIAANAPTHASRLLGGAITMIFFTYAFVNM
                     GMVSGILPVVGVPLPFISYGGTALVTLCLGIGILMSIQRSRFAEKT"
     gene            complement(4428..6473)
                     /gene="mrdA"
                     /locus_tag="PKN25_10675"
     CDS             complement(4428..6473)
                     /gene="mrdA"
                     /locus_tag="PKN25_10675"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNV91406.1"
                     /translation="MTEFRAPAVELARFRLRVVVAAVFVLVCFGLLAARFHHLQVKRH
                     DYFLTRAEDNRIALLPVVPHRGTIVDRKGVVLARNYATYTLEITPSQVRDLDATLDEL
                     ASLLPIDGRDRRRFRKLYEESRNFESVPLRSRLTDEEVARVVSQRYRLPGVDVKARLL
                     RDYPQGTTASHVIGYIGRITERDVERIEEAGNAANYRGSQHMGKAGLEQSYEHELHGT
                     TGFEQVEVNAGGRAVRALSHTPATSGNDLELTLDIELQKVAEKAFGDRRGALVAIEPS
                     TGGVLALASMPTYDPNLFVDGISTQDWKALNDSPDHPMLHRAIYSTYPPGSTFKPFMA
                     LAGLETGKRTARQAIHDVGYFNSGGHRFMDDKIGGHGMVDLHKSIVVSCNTYYYQLAN
                     DLGIEGIAGFMAPFGFGERTGIDLPGEATGVLPSPEWKRKRFKKAEQQRWFGGETISV
                     GIGQGYNAYTPLQLANALAALVNRGRLYRPHVVKYVVDSRTGERRAVEPEPLRQIPLR
                     DAHLRAVLDAMVDVNRTGTGKRAFKGAPYTVGGKTGTAQVFSLRGQRYVEGRVRERLR
                     DHSWFVAYAPAENPKIALAVLVENGGFGAQSAAPIARQVIDYYLLDQRAGAPAAEDEE
                     GAESEEGAEGGGEASPATPAVESATPAEEPTAPPQPRPVPQPAAVPAPQPGVARSTR"
     gene            complement(6486..7007)
                     /gene="mreD"
                     /locus_tag="PKN25_10680"
     CDS             complement(6486..7007)
                     /gene="mreD"
                     /locus_tag="PKN25_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNV91407.1"
                     /translation="MHPTNRSSRILQPVKLWFVYLSLFVALGLEYIPTGRTPGLPDWV
                     ALALAFWCVREPLAIGMGAGFAFGLLMDVGLGAAMGQHALAYVVLAYLANALARRVLW
                     FPAWQQALHVLPLLLLAQAIMALVRLVAGAEFPGWAYFLSSFGSALLWTPLSFLLLLP
                     QYQPDERDDNRPI"
     gene            complement(7011..7889)
                     /gene="mreC"
                     /locus_tag="PKN25_10685"
     CDS             complement(7011..7889)
                     /gene="mreC"
                     /locus_tag="PKN25_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNV91408.1"
                     /translation="MSLVGHQPPPIFRRGPAPLARLFVFVALCLVLLVADLRFRYLEV
                     LRNALSVVTYPLQMAASTPADVVRNASRYFGTLVDVQLENAELRRHQLGAGERLLRFE
                     QLEQENLHLRELLQMSQRVQTKSIAADILYNAPDPFARKVILDRGTQQGVEAGLAVLD
                     ANGVIGQVTRVHPVQSEVTLLTDRNQSIPVSVVRNGVRGVLYGVGRGMLEMRHVLAEV
                     DIQPGDRLVTSGLDGIFVPGLPVATVTRVDRDQDAFARIECEPLAAIERSVQVLVIGR
                     AAYPPPPPPPEPAGGR"
     gene            complement(7961..9004)
                     /locus_tag="PKN25_10690"
     CDS             complement(7961..9004)
                     /locus_tag="PKN25_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNV91409.1"
                     /translation="MFGFLRSYFSNDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRTE
                     GGPNAKRSIQAVGHQAKQMLGKTPGNITAIRPMKDGVIADFVVTEQMIKQFIKKVHDS
                     RLLSPSPRIIICVPCGSTQVERRAIRDAALAAGASQVFLIEEPMAAAIGAGLPVSDAT
                     GSMVVDIGGGTTEVGVISLGGIVYSGSIRVGGDKFDESIVNYIRRNYGMLIGETTAEK
                     IKKQIGSAFPGSEVREMEVKGRNLAEGIPRSFTISSNEILEALTEPLNQIVSAVKIGL
                     EQTPPELGADIAERGMMLTGGGALVRDLDRLLMEETGLPVVVAEEPLTCVARGCGMAL
                     DKMDKLASIFTSE"
     gene            9174..9461
                     /gene="gatC"
                     /locus_tag="PKN25_10695"
     CDS             9174..9461
                     /gene="gatC"
                     /locus_tag="PKN25_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNV91410.1"
                     /translation="MSLSNDQVGHIARLARIALSDTELEATRAKLDGIFGLIEQMQAV
                     DTTGVEPMSHPQEFPTRLRPDVVTETDRRDAFQKVAPQTEAGLYLVPKVIE"
     gene            9547..11019
                     /gene="gatA"
                     /locus_tag="PKN25_10700"
     CDS             9547..11019
                     /gene="gatA"
                     /locus_tag="PKN25_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNV91411.1"
                     /translation="MINASLPELRRALDARQISAVELAGLFLDRIDALNPALNAFITV
                     DREGALAAARAADARIAAGSAGPLTGIPLAHKDVFCTEGVLTTCGSKMLANFVSPYDA
                     HVVSLLEAAGAVSLGKANMDEFAMGSSNESSYFGAVKNPWNLGRIPGGSSGGSAAAVA
                     ARLAPIATGTDTGGSVRQPAAYCGITGIKPTYGLVSRYGMIAYASSLDQGGAFGASAE
                     DCALLLGAMTGFDARDSTSLERPTEDYAAALAGPACARPLEGLRIGLPREFFAEGMSD
                     DVRAAVEAAVEQYRVLGATTVEVSLPNAKLAIPAYYVIAPAECSSNLSRFDGVRYGHR
                     AADYGDLADMYAKSRAEGFGAEVKRRILVGTYVLSHGYYDAYYLQAQRLRRLIAQDFQ
                     AALQHCDLIAGPTTPTTAWTLGEKADDPVQMYLSDIYTIAVNLAGLPGLSHPVGFGAD
                     GLPVGLQLIGDYFAESRLLHAAHRFQHATDWHARRPACAA"
     gene            11030..11545
                     /locus_tag="PKN25_10705"
     CDS             11030..11545
                     /locus_tag="PKN25_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91412.1"
                     /translation="MSALSRPTLRPLHGRLAALAAAVLLSACALPVLPPSDAPISDEV
                     LASPSSAGKHLRPMPWRPLDVRADCRFRDEAGYTTRITLDIAQSKVNAFNADVDIPRR
                     GSCSFHGPFTQTREKPSVQLRAPDGCTVDIFEQGKAVTVGFANCASRCTRGASDYLWP
                     ILVDRTNGQCH"
     gene            11583..>12443
                     /gene="gatB"
                     /locus_tag="PKN25_10710"
     CDS             11583..>12443
                     /gene="gatB"
                     /locus_tag="PKN25_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNV91413.1"
                     /translation="MSRSDWEVVIGLEVHAQLDTASKIFSGASTAFGAEPNRQASAVD
                     IALPGVLPVLNRGAVERAIRFGLAIGAHVAEKSVFARKNYFYPDLPKGYQISQMDLPV
                     VVGGTITIRVGEGESAYEKTVRLTRAHLEEDAGKSLHEDFHGMTGIDLNRAGTPLLEI
                     VSEPDMRSSAEAVAYARALHALVRWIDICDGNMQEGSFRCDANVSVRRKGATEFGTRR
                     EIKNLNSFRFLQQAIDYEVQWQIDTLEDGGRIEQATVLFDPDTGETRMMRSKEDAHDY
                     RYFPDPDLLPL"
BASE COUNT         1981 a         4299 c         4368 g         1795 t
ORIGIN      
        1 tcgccgaccg cgtccacgct ggcatcgatg ccgaaatcct tcatgcggat cgtcttcagc
       61 ccctcgtagc cacaggggtt gatccagccg aagggcgcga ggtcggcgtc cacgttgatc
      121 gccaggccgt ggtagctgca gccgttgcgg acgcgcaggc cgagcgcggc gatcttgtcg
      181 ccggcgatgt agaccccagg cgcgccatcc ttgcgttgtg cggcgaggcc gtaatcggcc
      241 agggtgtcga tgatcgcctg ctccatcaga tgcaccagct cgcgcacctt gagcctgcgc
      301 cgcggcagat cgagcagcag gtaggccacg agctggccgg ggccgtggta agtgatctgg
      361 ccgccgcggt cgatgcgcac cagcgggata ttggccgggt tctgcagcag gtgctcgggc
      421 ttgccggcct ggccgagggt gtacaccggc gggtgctgca gcagccagat ctcgtcggac
      481 gtgtcggcgc cgcgcgcggc ggtgaacacg cgcatcgcct cgagcgccgg tgcgtactcc
      541 accaggccga ggcgcttgac gatcacagca ccaccttgac catcgggtgc gcggtgagct
      601 cgcggtacat cgcgtccacc tgcacctgcg acagcgcgcg gaaggtgcag gtcaccgcga
      661 ggtaattgcc cttgctcgag ggccgcatct cggcgccggc aggatcgaag tcgggcgcgt
      721 ggcgcagcac cacctcgatc accgcctggg cgaagccgtc cacccgcgcg cccatcacct
      781 tgatgggaaa gtcgcaggga tattcgatca ggctctggcg cgtctgcgcg tcctgctggt
      841 cagccatggc ggatcaccgt attcgtggat tcgcggtccg gagcgcgcat cacaagctct
      901 tgaagaacat cacgatcgca tcccacaggc ggccgaagaa gcccgccacc tcgacgtcct
      961 gcagcgccac caccgggtac tggccgagcg gcttgccgtc gaccgcgacc gtcagggtgc
     1021 cgacctcctg gccgcgctgc agcggcgcca ccaggggctg gcggctctcc aggctgacct
     1081 ggatcttgtt tgccgcctcc ttgggcacgg tgagcgcgaa gtccttcgtg aacccgaccg
     1141 gcagctcgtt gagcttgccc ttccagacac ggaacttcga cagcgcctcg tcggcggagt
     1201 agagcttgac cgtctcgtag aactggtagc cgaagttgag cagcttgagc gactcctgcg
     1261 cgcgcacgga gtccgaggcc gcacccagca ccaccgagac caggcggcgc tcgccgcgac
     1321 gggcggtgga cacgaggcag tagccggcgc tgctggtgtg gccggtcttc atgccgtcga
     1381 cggaggggtc catgtacagc aggcggttgc ggttgggctg cttgatgccg ttgtaggtga
     1441 attccttgag tgaatacagg ccgtagtact cggggaagtc gcggacgatc gcgctcgcca
     1501 gcacggcgag gtcgcgcgcg ctggtgtaca gctgcggatc gggcaggccg gtggaattgg
     1561 tgaagtgggt gttggtcatg cccaggcgct gcgcctcgcg gttcatcagc gcggcgaagg
     1621 cttcctcgct gccggcgatg gtctcggcga gcgccacgca ggcgtcgttg cccgactgca
     1681 cgatcacgcc gcggatgagc tcatccacac tgaccggctt gttgggctcg atgaacatgc
     1741 gcgagccttc catgcgccag gccttggtgg agaccggcac cacctggtcg ggcttgagcg
     1801 agccttcctt gagcttggag aaggtgatgt aggccgtcat cagcttggtc agcgaggccg
     1861 gctcgatgcg ggcgtcggcg tccttctcgg cgagcgtctg cccggtggca tggtcgacca
     1921 gcgcccaggc cttggcggcc agcgcggggg gcggcaccgt ctgcgcgagc gcggacagcg
     1981 agaacagcga gacgagtacg gcaatgagga aacgcatcgg gaaaacctgg agaagcggga
     2041 atgggaacgg cggattatag gcgccgcgca tggacgggca aaaaatggaa agcctttgtt
     2101 gcaacccggg cgcggtgcgc gacggtcagc gcaccaccac gaagggcttg agcttgagca
     2161 cgttgcccat gcgctcggcc gcatcgcgcg ccgcctcgac cgtgtcgtag ggaccggcgt
     2221 ggaggcggaa acgttcgcca tcggcgaaca attcgaaacg ctcggccaga tccgaggcct
     2281 ggctgcgcac cgtgtcgcgg aagccttcgg cgttggcgaa cgaggagaag gcgccgagct
     2341 gcaggaagat gcccgcgctc gccgacgcga ccggcggcgc gaagccgccc gcggcaccgc
     2401 agacggggcc ttcctcgcac tcgggcgggg cgagggcgat cgcgctgccg gctgcccgcg
     2461 gggggctcgc gaccggcgcg ggcagcggag cctgcgccac cggcaagggg gcgagtgcct
     2521 tcatcccggt cgccggcccc gaccccggca tgaggccctc gttggcgcgc gccggcagcg
     2581 gcggcaccgg gcgcgccatg gcgacgagcg gcgcctcgcc gggcaggatc tgctcgacct
     2641 cgacctcggc gctgccgccg ttgacatagc ccagcttgtg cgccgcggca taggacaggt
     2701 cgatcacccg gccgcgcagg aaggggccgc gatcgttcac ccgcaccacc accgaacggc
     2761 cgttggcgag gttggtcacg cgcgcgtagc tcgggatcgg cagggtcgga tgcgcggccg
     2821 tcatcgcgta catgtcgtag ggttcgccac tcgaggtcgg gttgccgtgg aagcggcggc
     2881 cgtaccagct gccgtggccg cgctggcgga aggggcgcag ctcggtggcg ggcacgaagc
     2941 tctgccccat gacgtggtag gggcggttgg cgaagcgatg caggggctcg tcgcgcggct
     3001 ccgcgtcggg gatcgcgtcg aggttgtcgg ggatgtcgtc gcccgggccg tcgtccttgt
     3061 agtagccgcc accgcgcacc gggctgcgtc cgggctttcc ggcctgcgcc ggcacctcgg
     3121 cgaccggtcc ttcgcgcttg ggcgcggagc tgcaggccga gagcagcgcg acggcgcagg
     3181 cgaggcctgc cgccaccagg gcacgcggcg cccgcgctgc ctgcgggcgg gctgcgaagg
     3241 ctgcgggaag cacgggagga actggcgtca tggtcggtcg gggttctctc aggtcttttc
     3301 cgcgaagcgg ctgcgctgga tgctcatgag gatgccgatc cccagacaga gcgtgacgag
     3361 cgcggttccg ccatagctga tgaaaggcag cggcaccccc accaccggca ggatgccgct
     3421 caccatgccc atgttcacga aggcataggt gaagaagatc atcgtgatcg ccccgccgag
     3481 caggcgcgaa gcgtgggtgg gcgcattcgc ggcgatgtgg aagccgcgca gcagcagcag
     3541 cacgtagatg accagcagca gcacggcgcc gacgaggccg aactcctcgg cgagcacggc
     3601 gaagatgaag tcggtgtggc gctcgggcag gaaggcgaga tgagtctggg tgccgtccat
     3661 ccagcccttc ccggtgagcc cgccggagcc gatcgcgatc gtggactgga tgatgtggaa
     3721 gcctcgtccg agcggatcgc gcgtggggtc gagcagggtg cacacgcgct gcttctggta
     3781 ttcgcgcagc acctgccagt ccacctcggg ctggcacagg gtgtcgccga aggcgacgat
     3841 cgagcccagc ccgatcacgc ccaccagcac cagcgggatg atcagcttcc acgacaggcc
     3901 ggcgaagtag atcacgtaga tgccggatgc ggccacaagc aggctggtgc cgaggtcggg
     3961 ctggatcagg atcagcccca ccggcacgag cagcagcacg ccggcgagga tgaagtcgac
     4021 gaggcgggtc tggccctcgc gcatctggaa gaaccaggcc agcatcagcg gcatcgcgat
     4081 cttcatcaat tccgagggct ggatgcgcgc cacgccgaga tcgagccagc gctgcgcacc
     4141 cttggagatc tctccgaaca gctccaccgc cagcagcagc accacgccga gcaggtagag
     4201 cggtaccgcc atcgacagca ggcgctgcgg cttgagccag gccagcagcc acatgccggc
     4261 gagcgcgatg ccgatatggg tgatctgcga atccaggcgc tccggcgagg cgctctgcat
     4321 cagcacgtgg gcgtagccga gcaggacggc gagcagcagc agcagcaggg ggtcgagcgg
     4381 gcgcaggaag ccgaggacga gcgcgagcgg gttgaagcgg ttgtcgttca tcgtgtgctc
     4441 ctcgccacgc cgggctgcgg cgcgggcacc gccgcgggct gcggcaccgg gcgcggctgc
     4501 gggggcgcgg tcggctcctc cgccggcgtc gcggactcca ccgccggcgt cgccggcgac
     4561 gcctcgccgc cgccctccgc gccctcctcg ctctcggcgc cctcctcgtc ctcggcggcc
     4621 ggcgcgccgg cacgctggtc gagcaggtag tagtcgatca cctggcgcgc gatcggcgcc
     4681 gccgactggg cgccgaagcc gccgttctcg accagcaccg cgagcgcgat cttcggattc
     4741 tcggccgggg cgtaagcgac gaaccaggag tggtcgcgca ggcgctcgcg cacgcggccc
     4801 tcgacgtagc gctggccgcg cagcgagaac acctgggcgg tgccggtctt gccgccgacc
     4861 gtgtaaggcg cccccttgaa cgcgcgcttg ccggtgcccg tgcggttgac gtcgaccatc
     4921 gcgtcgagca ccgccctcag gtgcgcatcc cgcagcggaa tctggcgcag gggctccggt
     4981 tcgaccgcac gccgctcgcc ggtgcgcgaa tcgaccacgt acttgaccac atgcgggcgg
     5041 tacagacggc cccggttgac cagcgcggcg agcgcgttgg cgagttgcag cggcgtgtag
     5101 gcgttgtagc cctggccgat gcccaccgag atcgtctcgc cgccgaacca gcgctgctgc
     5161 tcggccttct tgaagcgctt gcgcttccac tccggcgagg gcagcacgcc ggtggcctcc
     5221 ccgggcaggt cgatgccggt gcgctcgccg aagccgaagg gggccatgaa gccggcgatg
     5281 ccctcgatgc cgaggtcgtt ggcgagctgg tagtaatagg tgttgcacga caccacgatc
     5341 gacttgtgca ggtccaccat gccgtggccg ccgatcttgt cgtccatgaa acggtggccg
     5401 ccggagttga agtagccgac gtcgtggatc gcctgccgcg cggtgcgctt gccggtctcg
     5461 aggccggcga gcgccatgaa gggtttgaag gtggagccgg gcggataggt ggaatagatc
     5521 gcgcggtgca gcatcgggtg gtcgggcgaa tcgttgagcg ccttccagtc ctgggtcgag
     5581 atgccgtcga cgaagaggtt ggggtcgtag gtcggcatcg acgccagcgc gagcacgccg
     5641 ccggtcgagg gctcgatcgc caccagggcg ccgcggcgat caccgaaggc cttctcggcc
     5701 accttctgca gttcgatgtc gagggtcagc tcgagatcgt tgcccgacgt cgccggagtg
     5761 tgcgacagcg cgcgcaccgc gcgcccgccg gcgttgacct cgacctgctc gaagccggtg
     5821 gtgccgtgca actcgtgctc gtaggactgc tccaggccgg ccttgcccat gtgctggctg
     5881 ccgcggtagt tggccgcgtt gccggcctcc tcgatgcgct cgacgtcgcg ctcggtgatg
     5941 cggccgatgt agccgatcac atgcgatgcg gtagttccct gcgggtagtc gcgcagcagg
     6001 cgggccttca cgtcgacgcc gggcaggcgg tagcgctgcg agaccacgcg tgcgacctcc
     6061 tcgtcggtga gccggctgcg cagcggcacg ctctcgaagt tgcggctctc ctcgtagagc
     6121 ttgcggaaac ggcggcggtc gcgtccgtcg atcggcagca ggctggcgag ctcgtcgagc
     6181 gtggcgtcga ggtcgcgcac ctgcgagggg gtgatctcga gcgtgtaggt ggcgtagttg
     6241 cgcgccagca ccacgccctt gcgatcgacg atggtgccgc ggtgcggcac caccggcagc
     6301 aacgcgatgc ggttgtcctc ggcgcgggtg aggaagtagt cgtggcgctt gacctgcagg
     6361 tggtggaagc gcgccgcgag cagaccgaaa cagacgagca cgaacaccgc cgccaccacg
     6421 acgcgaagcc ggaagcgggc cagttcgacc gcgggcgcac gaaactcggt catcgcccgc
     6481 ccgcctcaga tcgggcgatt gtcatcgcgc tcgtccggct ggtactgcgg cagcagcagc
     6541 aggaaggaca gcggcgtcca cagcagcgcg ctgccgaagc tcgacaggaa atacgcccac
     6601 ccggggaact ccgcccccgc caccaggcgc accagcgcca tgatcgcctg cgccagcagc
     6661 agcaggggca acacgtgcag cgcctgctgc caggcgggga accacagcac gcggcgggcg
     6721 agcgcgttgg ccagataagc cagcacgaca taggcgagcg cgtgctgccc catcgccgcg
     6781 ccgaggccga cgtccatcag caggccgaag gcgaaacccg cgcccatgcc gatcgcgagc
     6841 ggctcgcgca cgcaccagaa ggccaacgcc agcgccaccc agtcgggcag gccgggcgtg
     6901 cgtccggtgg ggatgtactc caggccgagc gcgacgaaca ggctcaggta gacgaaccac
     6961 agcttgaccg gctgcaggat gcggctggaa cggttggtgg ggtgcatggt tcagcgccct
     7021 cccgcgggtt cgggcggcgg cggaggcggc ggatacgcgg cgcgcccgat caccagcacc
     7081 tgcacgctgc gctcgatcgc cgccagcggc tcgcattcga tgcgcgcgaa ggcgtcctgg
     7141 tcgcgatcga cccgggtcac cgtcgccacc ggcaggccgg gcacgaagat gccgtcgagg
     7201 cccgaggtga cgaggcggtc gccgggctgg atatcgacct cggcgagtac gtggcgcatc
     7261 tccagcatgc cgcggccgac gccgtagagc acgccgcgca cgccgttgcg caccacgctg
     7321 accggaatcg actggttgcg gtcggtgagc agggtgactt cggactggac cggatggacg
     7381 cgggtgacct ggccgatcac gccgttggcg tcgagcaccg cgagaccggc ctcgacaccc
     7441 tgctgggtgc cacggtcgag gatcaccttg cgcgcgaagg ggtcgggcgc gttgtagagg
     7501 atgtcggcgg cgatgctctt cgtctgcacg cgctgcgaca tctgcagcag ctcgcgcaga
     7561 tgcaggttct cctgctcgag ctgctcgaag cgcagcaggc gttcgccggc gccgagctgg
     7621 tggcggcgca actcggcgtt ctcgagctgc acatcgacca gggtgccgaa atagcgcgag
     7681 gcgttgcgca ccacgtcggc cggcgtcgac gccgccatct gcagcgggta ggtgaccacg
     7741 gacagcgcgt tgcgcagcac ctcgagatag cggaagcgca ggtcggccac gagcagcacg
     7801 aggcacagcg cgacgaacac gaacaggcgc gccaagggtg ccgggccgcg ccggaagatg
     7861 ggagggggct gatggccgac gagagacatc cgctacgaac cagggccgtc gggtggagag
     7921 gagaaacgcg ccccggcgcg cgcaccgggg ccggagagcg tcactcggac gtgaagatgg
     7981 aggcgagctt gtccatcttg tcgagcgcca tgccgcagcc gcgcgcgacg caggtcagcg
     8041 gttcctcggc cacgaccacc ggcaggccgg tctcttccat cagcaggcgg tcgaggtcgc
     8101 gcaccagggc gccaccgccg gtgagcatca tgccgcgctc ggcgatgtcg gcgccgagct
     8161 cgggcggcgt ctgctcgagg ccgatcttga ccgcggagac gatctggttg agcggctcgg
     8221 tgagcgcctc gaggatctcg ttggacgaga tcgtgaagct gcgcgggatg ccctcggcca
     8281 ggttgcggcc cttgacctcc atttcgcgca cctcggaacc ggggaaggcg gaaccgatct
     8341 gcttcttgat cttctcggcg gtggtttcgc cgatcagcat gccgtagttg cggcggatgt
     8401 aattgacgat ggactcgtcg aacttgtcgc cgccgacacg gatgctgccg gagtacacga
     8461 tgccgccgag cgagatgaca ccgacttcgg tagtgccgcc gccgatgtcg accaccatcg
     8521 agccggtcgc gtccgacacc ggcaggccgg cgccgatcgc cgcggccatg ggttcttcga
     8581 tgaggaagac ctgcgaggcg ccggccgcca gggccgcatc gcggatcgcg cggcgctcga
     8641 cctgggtcga gccgcaaggc acgcagatga tgatgcgcgg ggacggcgac agcaggcggg
     8701 agtcgtgcac cttcttgatg aactgcttga tcatctgttc ggtaacgacg aagtcggcga
     8761 tcacgccgtc cttcatcggc cggatggcgg tgatgttgcc cggcgtcttg ccgagcatct
     8821 gcttggcctg gtggccgacc gcctggatgg agcgcttggc gttgggcccc ccctcggtac
     8881 ggatcgccac caccgagggt tcgtccagca cgatgccctt gccgcgcacg tagatcaggg
     8941 tattggccgt gccgaggtcg atcgcgagat cgttggagaa ataggagcgc aggaaaccga
     9001 acatgaagcc ttccgaaacc ggtggagtgg tcgagccgcg cgcagtcggc gccctgggcg
     9061 cgctgcaaca cgaaaacccc gcccgctacc gcgaagcggt gtagccccgg gcggggcaaa
     9121 gacgattatg ataacctaca aacctttgtg gattcagcag gttttgttac gccatgtcgc
     9181 tctccaatga ccaggtcggg cacatcgccc gcctcgcgcg catcgcgctt tccgacaccg
     9241 aactcgaagc cacgcgcgcc aagctcgacg gaatcttcgg cctcatcgag cagatgcagg
     9301 cggtcgacac caccggcgtg gagccgatga gccacccgca ggagtttccc acccgcctgc
     9361 gtcccgacgt cgtcaccgag accgaccgcc gcgacgcctt ccagaaggtc gcgccgcaaa
     9421 ccgaggccgg cctctacctg gtgccgaaag tcatcgaatg atccgctccg cggccaccgt
     9481 tcccgtcgct gcgcccgcgc ccgtccgccc ggagcgcgcc gctgcgccaa cctgcccgac
     9541 cgtaaaatga tcaacgcttc cctcccggaa ctgcgccgcg cgctcgacgc ccggcagatc
     9601 tccgccgtcg aactcgcggg cctgttcctc gaccgcatcg acgcgctcaa ccccgcgctc
     9661 aacgccttca tcaccgtcga ccgcgaaggc gcgctcgccg ccgcgcgcgc ggccgacgcg
     9721 cgcatcgccg ccggcagcgc cgggccgctc accggcatcc cgctcgcgca caaggacgtg
     9781 ttctgcaccg agggcgtgct caccacctgc ggctcgaaga tgctggccaa cttcgtcagc
     9841 ccctacgacg cccacgtggt gagcctgctc gaggccgccg gcgcggtaag cctgggcaag
     9901 gccaacatgg acgagttcgc catgggctcg tcgaacgaga gctcgtactt cggcgcggtg
     9961 aagaacccgt ggaacctcgg ccgcatcccc ggcggctcgt ccggcggctc ggccgccgca
    10021 gtggcggcgc gcctggcgcc gatcgccacc ggcaccgaca ccggcggctc ggtgcgccag
    10081 cccgcagcct actgcggcat caccggcatc aagcccacct acggcctggt gagccgctac
    10141 ggcatgatcg cctacgcctc ctcgctcgac cagggcggcg ccttcggcgc cagcgccgag
    10201 gactgcgcgc tgctgctcgg ggcgatgacc ggcttcgacg cgcgcgactc caccagcctg
    10261 gagcgcccga ccgaggacta cgccgccgcg ctcgccggcc ccgcttgcgc ccgcccgctc
    10321 gaaggcctgc gcatcggcct gccgcgcgag ttcttcgccg agggcatgtc cgacgacgtg
    10381 cgcgccgcgg tcgaggcggc ggtcgagcag taccgcgtgc tcggcgccac cacggtcgag
    10441 gtctcgctgc ccaacgcgaa gctggcgatc ccggcctact acgtgatcgc gccggcggaa
    10501 tgctcgagca acctcagtcg cttcgatggc gtgcgctacg gccaccgcgc cgccgactac
    10561 ggcgacctcg ccgacatgta cgcgaagagc cgcgccgagg gcttcggcgc cgaagtcaag
    10621 cgccgcatcc tggtcggcac ctacgtgctc tcgcacggct actacgatgc ctactacctg
    10681 caggcgcagc gcctgcgccg cctgatcgcg caggacttcc aggccgcgct gcaacactgc
    10741 gacctgatcg ccggaccgac cacgccgacc acggcctgga cgctcggcga gaaggccgac
    10801 gacccggtgc agatgtacct gtccgacatc tacaccatcg cggtgaacct cgccggcctg
    10861 cccggactgt cgcacccggt gggcttcggc gccgacggcc tgccggtggg cctgcagctg
    10921 atcggcgact atttcgccga atcccgcctg ctgcacgccg cacaccgctt ccagcacgcc
    10981 accgactggc acgcgcgccg gccggcgtgc gccgcgtgag ttccacgcag tgagcgccct
    11041 gtcccgcccg acgctgcgcc cgctccacgg ccgcctcgcc gcactggcgg cggcggtgct
    11101 gctgtcggcc tgcgcgttgc cggtgctgcc gccttccgac gcgccgatca gcgacgaggt
    11161 gctggccagc ccgagctcgg ccggcaagca cctgcgcccg atgccgtggc ggccgctcga
    11221 cgtgcgcgcc gactgccgct tccgcgacga ggccggctac accacgcgca tcacgctcga
    11281 catcgcgcag tccaaggtca acgccttcaa cgccgacgtc gacatcccgc gccgcggcag
    11341 ttgcagcttc cacggcccat tcacgcagac gcgcgagaag ccgagcgtgc agctgcgcgc
    11401 gcccgacggc tgcacggtgg acatcttcga gcagggcaag gccgtcaccg tcggcttcgc
    11461 caactgcgcc agccgctgca cccgcggcgc ctccgattac ctgtggccga tcctcgtcga
    11521 tcggaccaac ggccagtgcc actgaccccg gtggccggct gcacgcaaca cgagaacaca
    11581 ccatgagccg atccgattgg gaagtcgtca tcgggctgga agtccacgcc cagctcgaca
    11641 ccgccagcaa gatcttttcc ggcgccagca ccgccttcgg cgccgagccc aaccgccagg
    11701 cgagcgcggt ggacatcgcg ctccccggcg tgctgccggt gctcaaccgc ggcgcggtcg
    11761 agcgcgcgat ccgcttcggg ctggcgatcg gcgcccacgt ggcggaaaag agcgtgttcg
    11821 cgcgcaagaa ctacttctac cccgacctcc ccaagggcta ccagatcagc cagatggacc
    11881 tgccggtggt agtcggcggc acgatcacca tccgcgtcgg cgagggcgag tcggcgtatg
    11941 agaagaccgt gcgcctgacc cgcgcccacc tcgaggaaga cgcgggcaag agcctgcacg
    12001 aggacttcca cggcatgacc ggcatcgacc tcaaccgcgc cggcacgccg ctgctcgaga
    12061 tcgtctccga gcccgacatg cgctcgtccg ccgaggccgt ggcctacgcg cgcgcgctgc
    12121 acgcgctggt gcgctggatc gacatctgcg acggcaacat gcaggaaggc tcgttccgct
    12181 gcgacgccaa cgtctcggtg cgcaggaagg gcgcgaccga gttcggcacc cggcgcgaga
    12241 tcaagaacct caactccttc cgcttcctgc agcaggcgat cgactacgag gtgcagtggc
    12301 agatcgacac cctcgaggac ggcggccgca tcgagcaggc caccgtgctg ttcgaccccg
    12361 acacgggcga gacgcgcatg atgcgcagca aggaagacgc ccacgactac cgctacttcc
    12421 ccgaccccga tctgctgccg ctg
//
LOCUS       DAOQBS010000092        12363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4233_length_12363_cov_14.705476, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000092 DAOQBS010000000
VERSION     DAOQBS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12363
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4233_length_12363_cov_14.705476"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKN25_10715"
     CDS             complement(<1..592)
                     /locus_tag="PKN25_10715"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV91414.1"
                     /translation="MTEAPRPESAPAEAELRFADLQLPAPLLSALAEVGYETPSPIQA
                     ACIPQLLAGHDILGEAQTGTGKTAAFALPMLAKLDLADTRPQVLVLTPTRELAIQVAE
                     AFAKYAHHLKNFHVLPIYGGQSMVVQLRQLSRGAQVVVGTPGRVMDHLERESLKLDAL
                     KAIVLDEADEMLRMGFIDDVEWILEHTPAERQTALFS"
     gene            complement(836..1045)
                     /locus_tag="PKN25_10720"
     CDS             complement(836..1045)
                     /locus_tag="PKN25_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNV91415.1"
                     /translation="MSTQTGTVKWFNDAKGFGFITPEGGGDDLFAHFSEIQSKGFKSL
                     AENQRVEFEVKTGPKGLQAANIRPL"
     gene            1328..2302
                     /gene="ylqF"
                     /locus_tag="PKN25_10725"
     CDS             1328..2302
                     /gene="ylqF"
                     /locus_tag="PKN25_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HNV91416.1"
                     /translation="MPIQWFPGHMASARKKAAEAMAMTDVVIEVVDARLPEASSNPMI
                     AELRRFRNRPCLKLLNKSDLADPEVTRAWMESYNRLPGVKAVAISAKSPAEVARVPAL
                     CRQLAPHRDDGVKPLRMMIMGIPNVGKSTLMNALLKRKVAKVGDEPAVTKHQQTLDLG
                     PGMTLTDTPGMMWPKIDHDADGYMLAASHAIGRNAVIDEEVAAFLGEILIARYPDRLA
                     VRYRLDAAAMVALDGPALVEAVGRRRGCLVKGGGLDLEKAAQILLQDYRDGALGRISL
                     ETPETRAHMIAAAQAAAAQAAAAGETGEEAAGAAEAGGEADASGGEGD"
     gene            2726..3631
                     /locus_tag="PKN25_10730"
     CDS             2726..3631
                     /locus_tag="PKN25_10730"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HNV91417.1"
                     /translation="MSVPSASRLHALNVDLHCHSTVSDGWLEPEALVLRAIANGVELL
                     ALTDHDELGGIASAQLVARAHGLAFVPGVEISVSFAGETVHIVGLGVDPGSAELCAGL
                     AALRAGRDGRARRIGDELARVGIPDAFAGACRFARNPALVSRAHFARHLVASGVMPDV
                     KTVFDHYLVRGKPGFVEHRWATLEQAVGWIRAAGGIAVVAHPARYRLSSVAMGELFDR
                     FVAAGGEAVEVVAGAHTEDEMRRFASLARQRGLLASRASDFHGVQESMVDVGRCNPLP
                     ADLEPVWSRLQATTAALAAAASSAR"
     gene            3659..4282
                     /locus_tag="PKN25_10735"
     CDS             3659..4282
                     /locus_tag="PKN25_10735"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNV91418.1"
                     /translation="MAQYFSLHPEQPQARLIRQAAEILRGGGLVALPTDSAYALACQV
                     GDAALLDRIRRIRGVDERHHFTLMCRDLSEIGTYARVDNSQYRLLKAVTPGPYTFILE
                     GTKELPRRVLHPKRKTIGLRVPEHAVVSALLAEIDGPLLTSTLILPGEDLPLSDPEEI
                     RDRLGKLVELVIEAGYCGPEATTVIDLTSGAPELVRAGRGPLEPFGL"
     gene            4310..4969
                     /locus_tag="PKN25_10740"
     CDS             4310..4969
                     /locus_tag="PKN25_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNV91419.1"
                     /translation="MDSLISTLAIWALPVVLAITLHEAAHGYVARHFGDPTAHLAGRI
                     TLNPLRHIDPVGTILVPAGILALSSLAGGGGILFGWAKPVPVNFSRLRNPKSDMLWVA
                     AAGPFVNFVMALGWALLFRIGATSEPGAYTLPVLEMADAGIRINAVLMALNLLPIPPL
                     DGGRIAVSLLPDRLAWQYARLEPYGFPILLVLLFTGVLGTLLWPLLTGFRYLLSLVFG
                     F"
     gene            4976..6178
                     /locus_tag="PKN25_10745"
     CDS             4976..6178
                     /locus_tag="PKN25_10745"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNV91420.1"
                     /translation="MYAERVLSGMRPSGRLHLGHYHGVLKNWVKLQEEFPCLFFVADW
                     HALTTHYDTPEVIENSVWDMLVDWLAAGVDPAQSTLFIQSQVPEHAELHLLLSMMTPL
                     GWLERVPTWKEQQERIEGRDLSTYGFLGYPLLQSADILIYRADKVPVGEDQVPHIEIT
                     REIARRFNHLYGREPGFEDKAKDAVRKLGAKRARMYHDLRTRFQQEGDDEALEQAKAL
                     LDETQNLSLGDRERLYGYLEGSGKMILVEPEALLTPAARMPGLDGQKMSKSYGNTVNL
                     REDAESVTRKVRTMQTDPARMRRTDPGEPERCPVWQLHAIYSGEDTLAWVQRGCRSAG
                     IGCLECKQPVLEAILKEQEVLHERAQPYLDDPSLLRSIIADGCERARRLAQETMRDVR
                     EAMGLDYG"
     gene            6216..7046
                     /locus_tag="PKN25_10750"
     CDS             6216..7046
                     /locus_tag="PKN25_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNV91421.1"
                     /translation="MNLPLDLVPIDTGPPLDVLARIYGEPLLELPKDLYIPPDALQVF
                     LEAFEGPLDLLLYLIRKANLDILDVPMAPLTAQYLEYVEAMRATHLELAAEYLLMAAV
                     LLEIKSRMLLPRPPRASAEEEDPRAELVRRLLEYEQMKLAAARLEALPRVERDFEWVG
                     VFVAEKVVERLPEVSLHDLQLAWLRIMKKARLNQSHRVGREQLSVREHMTAILRKLGD
                     GAFVVFDALFDVELGPPGLVVSFLAVLELVKEKLVEVTQNEAFAPIYVKLADGTRNDA
                     "
     gene            7024..7626
                     /gene="scpB"
                     /locus_tag="PKN25_10755"
     CDS             7024..7626
                     /gene="scpB"
                     /locus_tag="PKN25_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNV91422.1"
                     /translation="MAPATTPETWLRIVEAALLSAPGALPVSELRKLFDEDPGPDLVR
                     RLLDELRAQWFEAGRGVELVQLASGWRFQTRPEYQVYLDRLKEEKPPRYSRAVMETLA
                     IIAYRQPVTRGDIEDIRGVAVSPSVLKTLESRGWVDIVGHRDTPGRPALFATTRRFLD
                     EMGLRSLTELPALTELEKIMDLVDVSQTEPAAAPAPEEEV"
     gene            7574..8713
                     /locus_tag="PKN25_10760"
     CDS             7574..8713
                     /locus_tag="PKN25_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNV91423.1"
                     /translation="MSRKPNRPLRPPLKKKSDAIEILDRDPRESRGARDDDAQPGRKD
                     AHLNADPRGPRTPRPPRAGGAPAPRRPRIEGDPVRHPDAHRSALGQPEGGERPVRGRG
                     PGGGRNAPTTLSEPERLQKVLAQAGVASRREIEEWVVAGRISVNGLPASLGQKIGPGD
                     RVKVNGKLVPLRFTQRSPRVLIYHKPEGEIVSRDDPEGRPTVFERLPILRKGRWLAVG
                     RLDFNTSGLLLFTNDGDLANKLMHPRYELEREYAVRILGELTEEQVKSLTEGIQLEDG
                     PAKFNLLRDEGGEGANHWYRVTISEGRNREVRRMFEAVGLTVSRLMRVRYGSVELPAR
                     LKRGMWMEMPEAEACALAGLPPPQQSRQQDLRDKRPVKLHRTQAR"
     gene            8983..9417
                     /gene="rimP"
                     /locus_tag="PKN25_10765"
     CDS             8983..9417
                     /gene="rimP"
                     /locus_tag="PKN25_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNV91424.1"
                     /translation="MRADVEGLIEQVVTGLGYELVDIEFSPKGRLLRVFLDIERGITV
                     DDCATVSNQLQRVFEVENVDYDRLEVSSPGLDRPLKKLADFERFAGEQAQVRLSLPIG
                     NQRNFVGVIERVQDGAVALRTEKGEVLLPFDDIEKARLVPQF"
     gene            9445..10920
                     /gene="nusA"
                     /locus_tag="PKN25_10770"
     CDS             9445..10920
                     /gene="nusA"
                     /locus_tag="PKN25_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNV91425.1"
                     /translation="MSREILLLVDALAREKNVAKDIVFSALETALASATKKRIHDDAD
                     VVVSIDRDSGDYTSKRRWLVMLDEEVANDEAEMGIIDARELRADVQIGDYIEEELEPI
                     DFGRIGAQAAKQVILQKIRDAEREQVLNDFLDRKEFLVSGSIKRMERGNAIIEVGRME
                     AVLPRDQQIPRENLRVGDRVKAFLLRIDRGARGPQLVLSRTAPEFLMKLFELEVPEIE
                     DGLLELKACARDAGLRAKIAVKSNDQRIDPIGTCVGLRGSRVTAVRNEIAGEQIDIIV
                     WSQDPAQFVVAALQPAEVVSIVVDEESHAMDVVVDENNLAIAIGRSGQNVKLASELTG
                     WTINLMSEQESAEKTAQEQQGLRALFMEKLDVDEEVADILIEEGFSSLEEVAYVPLSE
                     MLEIEAFDEDTVNELRNRARNVLLTEAIVTEEQLEKVSDDLLGLEGMDKSLAATLAQQ
                     GIRTRDDLADLAVDELVEMAGIDEERAKALISVARAHWFEE"
     gene            10934..>12363
                     /locus_tag="PKN25_10775"
     CDS             10934..>12363
                     /locus_tag="PKN25_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HNV91426.1"
                     /translation="MEQMSVTQFAGELKMPAAVLLEQLKRAGVEKSGPTDLLTEQDKA
                     RLLDYLRRSHGDTQPKGKITLTRKQTSEIRATDSTGRARTVQVEVRKKRTFVKRDELA
                     EQAGVEAAAVEQAEIAEAVVEAPAPVTEAAAPAVEPAVEAAPVVAAAAPEVVEPPAAE
                     AVAPVAEAVAPVVEPAPAVAPPPAAPAASAARPAQQGGRHGRGERPQRQEPVAPQPQV
                     TTVLSKPLPLSEILSEEEVAARKRDEDRQRALKERQAADLRARQEREAAAKAAAEARK
                     AEEEARLRAEQQKKEEPAKAAAKPTTGTLHRPAKTEDKPASREVKRTAPAAPAREADG
                     ASKRRGGMKTRGEVGATTGSNWRGAGKGGGRHGRNQQDDRSSFQAPTEPIVREVHVPE
                     TITVADLAHKMAVKATEVIKVLMKMGSMVTINQVLDQETAMIIVEEMGHLAVAAKLDD
                     PDAFLEESEAHKDADVLPRAPVVTVMG"
BASE COUNT         1951 a         4274 c         4281 g         1857 t
ORIGIN      
        1 ccgagaacag cgcggtctgg cgctcggccg gggtgtgctc gaggatccac tcgacatcgt
       61 cgatgaagcc catgcgcagc atctcgtcgg cctcgtcgag cacgatggcc ttgagggcgt
      121 cgagcttgag gctctcgcgc tcgaggtggt ccatgacccg gcccggggtg ccgacgacca
      181 cctgggcgcc gcgcgagagc tggcgcagct gaacgaccat gctctggccg ccgtagatcg
      241 gcagcacgtg gaagttcttc aggtggtggg cgtacttggc gaaggcctcg gcgacctgga
      301 tcgctagctc gcgcgtgggg gtgagcacca ggacctgcgg gcgcgtgtcg gcgaggtcga
      361 gcttggccag catcggcagc gcgaaggcgg cggtcttgcc ggtcccggtc tgcgcctcgc
      421 cgaggatgtc gtggccggcg agcagttgcg ggatgcaggc ggcctggatc ggcgaggggg
      481 tctcgtaacc cacttcggcg agcgccgaca gcaggggcgc gggcagctgg agatcggcga
      541 agcgcagttc ggcttcggcg ggggcggatt cggggcgggg agcttcggtc atggcgtctc
      601 ggccgcgggg cggcgatggg cagcggcgct gcaacatcgc ggaggcgggc gattgcggcg
      661 cggcgggggt gatggggcgg ctcgtggccg cggcgttgcg gcgggtggcc gcgggcttgc
      721 gcgtccggcg cgctccgttg gtgcggaagg gcggcccgag gccgccgcaa atgcaaaaac
      781 gcggcgcagg gccgcgtttt gaatcaatcg atcgacgtcg aggacgtcgg gtcgattaca
      841 gcgggcggat gttggccgcc tgcaggccct tggggccggt cttgacttcg aattcgacgc
      901 gctggttttc ggccaggctc ttgaaaccct tgctctggat ttccgagaag tgggcgaaga
      961 ggtcgtcgcc gccgccttcc ggggtgatga agccgaagcc cttggcgtcg ttgaaccact
     1021 tgacggtacc ggtttgagtg ctcattttgc gttccatata aagaattgcg ggcttgcgcc
     1081 ccgaagtgca ggaacgatca agaaacgtaa tcagggaggc atcaaaccga cacgcgttct
     1141 cttggaacac gctggcaacc acaccaacaa ccactgcttg aatcgactgc gagcggcaag
     1201 atacacgctt ttgcgcggat gcgtgaactt atttttcagt tcgatcgcat gcggcgtgcg
     1261 cgtggggcgc gtgccggcgt ccggcagtgg gataatccgc cccccgacag tcaatccagg
     1321 tctctccatg cccatccagt ggttccccgg acacatggcc tccgcgcgca agaaggcggc
     1381 cgaggcgatg gccatgaccg acgtggtcat cgaggtcgtc gacgcgcgcc tgcccgaggc
     1441 cagcagcaac ccgatgatcg ccgagctgcg ccgctttcgt aaccgcccct gcctcaagct
     1501 gctcaacaag tccgacctcg ccgaccccga ggtcacacgt gcctggatgg agtcctacaa
     1561 tcgcctgccc ggcgtgaagg cggtggcgat ctcggccaag agcccggccg aggtggcgcg
     1621 cgtgccggcg ctgtgccgcc agctcgcgcc gcaccgcgac gatggcgtca agccgctgcg
     1681 catgatgatc atgggcattc ccaacgtcgg caaatcgacg ctgatgaacg cgctgctcaa
     1741 gcgcaaggtg gccaaggtgg gcgacgagcc tgcagtcacc aagcaccagc agacgctgga
     1801 tctcggtccc ggcatgacgc tcaccgacac gcccggcatg atgtggccca agatcgacca
     1861 cgatgccgac ggctacatgc tggcggcgag ccacgccatc ggccgcaacg cggtgatcga
     1921 cgaggaggtg gcggccttcc tcggcgagat cctcatcgcg cgctaccccg atcgcctggc
     1981 ggtgcgctac cggctcgacg ccgccgcgat ggtcgcgctc gacggcccgg cgctggtcga
     2041 ggcggtgggc cggcggcgcg gctgcctggt caagggcggt gggctggacc tggagaaggc
     2101 ggcgcagatc ctgctgcagg actatcgcga cggcgcgctc gggcgcatca gcctggagac
     2161 gccggagacg cgcgcgcaca tgatcgccgc cgcgcaggcg gccgcggctc aggctgccgc
     2221 tgcgggggag accggagagg aagccgccgg cgcggcggaa gcggggggcg aggccgatgc
     2281 ttccgggggc gagggcgact gatccgccgt cgccggcacg atctctatcc agcgcaacca
     2341 ctgcccacag gcgttgcgcg gcccgcgcag cgagccgtgc caggggcctt cccggcaggg
     2401 gccgcgctgc tccgtgggat gcagttcgac ggtgctgccg tcctcgccca cgcgccgcca
     2461 tccgccccag cgattgcagc tggggcagcg gcgcagttcg gcgacaggtg gggcgggggg
     2521 gacaaagctc atcgatcggg gccgggggtt cgggtgcaat gatacggcgg gcggacgcgg
     2581 gtgcggcagg cgtcgcaggc cacacggccg cacgatcgac ggctgctcgc gcctgcccgt
     2641 ccgcaggtcg ccgcatggcc catgccggcg cagtcccggc gcctttgata tcatcccgat
     2701 cctgccgttc ctcctccgga gtcccatgag cgttccgtca gcctcgcggc tgcacgccct
     2761 caacgtcgac ctgcactgcc attccaccgt ctcggacggc tggctcgagc ccgaagcgct
     2821 ggtgttgcgc gcgatcgcca acggcgtcga gctgctcgcg ctgaccgatc acgacgaact
     2881 tggcggcatc gcgtccgcgc agcttgtcgc ccgcgcccat ggcttggcct tcgtgccggg
     2941 cgtcgagatc tcggtctcct tcgccggcga gaccgtccat atcgtgggcc tgggcgtcga
     3001 tcccgggagc gccgaactgt gtgccggtct cgccgccctg cgtgccggac gcgacgggcg
     3061 cgcgcggcgc attggcgacg agctcgcgcg cgtcggcatc cccgatgcct tcgcgggtgc
     3121 ctgccgcttc gcgcgcaacc ccgcgctcgt gagccgcgcg cacttcgccc gccatctggt
     3181 cgcgagcggc gtgatgcccg acgtgaagac ggtcttcgac cactatctcg tgcgcggcaa
     3241 gcccggcttc gtcgagcacc gctgggcgac gctcgagcag gcggtgggct ggatccgcgc
     3301 cgccggcggc atcgcggtgg tcgcgcatcc ggcgcgctac cggctgtcct ccgtcgccat
     3361 gggcgagctc ttcgaccgct tcgtcgccgc cggcggtgag gccgtcgagg tggtggccgg
     3421 tgcgcacacc gaggacgaga tgcgtcgctt cgcgagcctc gcgcgccagc gcgggctgct
     3481 ggcctcgcgc gcgtccgatt tccatggtgt gcaggagagc atggtcgacg tcggccgctg
     3541 caatccgctg cctgccgacc tcgagccggt gtggagccgt ctgcaggcca ccaccgcggc
     3601 gctcgcggcg gccgcatcct ccgcgcgcta gggcgcgcgc cccatcgagc atccgatcat
     3661 ggcccagtat ttctcccttc atcccgaaca gccgcaggcg cggctgatcc gtcaggccgc
     3721 cgagatcctg cgtggcggcg gcctcgtcgc gctgcccacc gactccgcct acgcgctcgc
     3781 ctgccaggtc ggcgacgccg cgctgctcga ccgcatccgc cgcatccgcg gggtggatga
     3841 gcgccaccac ttcacgctga tgtgccgcga cctctccgag atcggcacct acgcccgggt
     3901 ggacaacagc cagtaccggt tgctgaaggc ggtcactccc ggcccctaca ccttcatcct
     3961 cgaaggcacc aaggagctgc cgcgccgcgt gctgcacccg aagcgcaaga ccatcggcct
     4021 gcgcgtgccc gagcacgcgg tggtgtcggc cctgctcgcc gagatcgacg gcccgctgct
     4081 gacctcgacc ctgatcctgc ccggcgagga cctgccgctc tccgacccgg aggagatccg
     4141 cgaccgtctc ggcaagctgg tcgagctggt catcgaggcc ggctactgcg gtcccgaggc
     4201 caccaccgtg atcgacctca cctcgggtgc gcccgagctg gtacgcgccg ggcgcggccc
     4261 gctcgagccc ttcggtctgt agaatccggg gtttgccacc gagcccgcga tggattcgct
     4321 gatctccacc ctggccatct gggccctccc ggtcgtgctc gcgatcacgc tgcacgaggc
     4381 cgcgcacggt tacgtggcac gccacttcgg cgaccccacc gcccacctcg ccgggcgcat
     4441 cacgctcaac ccgctgcgcc acatcgaccc ggtgggcacc atcctggtgc cggcgggcat
     4501 cctcgccctc agctcgctcg ccggtggcgg gggcatcctc ttcggctggg ccaagccggt
     4561 gccggtcaac ttctcgcgcc tgcgcaatcc caagtccgac atgttgtggg tggcggctgc
     4621 agggcctttc gtcaacttcg tcatggcgct cgggtgggcg ctgctgttcc gcatcggcgc
     4681 caccagcgag ccgggcgcct acacgctgcc ggtgctcgag atggccgacg ccggcatccg
     4741 gatcaacgcc gtgctgatgg cgctcaacct gctgccgatc ccgccgctcg acggcggccg
     4801 catcgcggtc agcctgctgc ccgaccggct ggcctggcag tacgcccggc tcgagcccta
     4861 cggcttcccg atcctgctcg tgctgctgtt caccggcgtg ctcggcaccc tgctgtggcc
     4921 gctgctcacc gggttccgct acctgctgtc cctcgtcttc ggattctgat gctccatgta
     4981 cgctgaacgt gtcctctccg gcatgcgccc cagtgggcgg ctccatctcg gccactacca
     5041 cggcgtgctc aagaactggg tgaagctgca ggaggagttc ccctgcctgt tcttcgtcgc
     5101 cgactggcat gcgctcacca cgcactacga cacccccgag gtgatcgaga acagcgtctg
     5161 ggacatgctg gtcgactggc tcgccgccgg ggtcgatccc gcgcagtcca cgctcttcat
     5221 ccagtcgcag gtgcccgagc acgccgagct gcacctgctg ctgtcgatga tgaccccgct
     5281 cggctggctg gagcgcgtgc cgacctggaa ggagcagcag gagaggatcg agggtcgcga
     5341 cctgtcgacc tacggcttcc tcggctatcc gctgctgcag tcggccgaca tcctgatcta
     5401 ccgtgcggac aaggtgccgg tgggcgagga ccaggtgccg cacatcgaga tcacccgcga
     5461 gatcgcacgc cgattcaatc acctctacgg ccgcgagccg ggcttcgaag acaaggccaa
     5521 ggacgcggtg cgcaagctcg gtgccaagcg tgcgcgcatg taccacgatc tgcgcacgcg
     5581 cttccagcag gagggcgacg acgaggcgct cgagcaggcc aaggccctgc tcgacgaaac
     5641 ccagaatctc tcgcttggcg accgcgagcg cctctacggc tacctggaag gcagcggcaa
     5701 gatgatcctg gtcgagcccg aggcgctgct taccccggcg gcgcgcatgc ccggcctgga
     5761 cgggcagaag atgtccaagt cctacggcaa caccgtcaac ctgcgcgagg acgccgagag
     5821 cgtcacgcgc aaggtgcgca ccatgcagac cgatcccgcg cgcatgcgcc gcaccgaccc
     5881 gggcgagccc gagcgctgcc cggtgtggca gctgcacgcg atctattccg gcgaagacac
     5941 cctggcctgg gtgcagcggg gttgccgcag cgcgggcatc ggctgcctgg agtgcaagca
     6001 gccggtgctg gaggcgatcc tgaaggagca ggaggtgctg cacgagcgcg cccagcctta
     6061 tctggacgat ccttcgctgc tgcgcagcat catcgccgac ggctgcgagc gcgcgcgccg
     6121 gctcgcgcag gagaccatgc gcgacgtgcg cgaggcgatg ggcctggact acggttgagc
     6181 ccgccgcacg ccgccttccc gatcgcagcc gcaccatgaa cctgccgctc gacctggtgc
     6241 cgatcgacac cggcccgccg ctcgatgtgc tcgcgcgcat ctacggcgag cccctgctcg
     6301 aattgccgaa agacctgtat attccgcccg atgcgctgca ggtcttcctg gaagccttcg
     6361 aggggccgct ggacctgctg ctctacctga tccgcaaggc caacctcgac atcctcgatg
     6421 tgccgatggc gccgctcacc gcgcagtacc tcgagtacgt cgaggcgatg cgggccaccc
     6481 acctcgagct cgccgccgaa tacctgctga tggccgcggt gctgctcgag atcaagtcgc
     6541 gcatgctgct gccgcggccg ccgcgcgcga gtgcggaaga agaggatccg cgcgccgagc
     6601 tggtgcgccg gctgctcgag tacgaacaga tgaagctcgc cgcagcacgg ctcgaggcgc
     6661 tgccgcgggt ggagcgcgac ttcgagtggg tgggggtgtt cgttgccgag aaggtggtcg
     6721 agcgcctgcc cgaggtcagc ctgcacgacc ttcagctggc ctggctgcgt atcatgaaga
     6781 aggcgcggct caaccagagc caccgtgtcg gccgcgagca gctctcggtg cgcgagcaca
     6841 tgacggcgat cctgcgcaag ctcggtgacg gcgccttcgt ggtgttcgac gccttgttcg
     6901 acgtcgagct cggcccgccc ggcctggtgg tgagctttct cgccgtgctc gagctggtga
     6961 aggaaaagct ggtcgaagtg acccagaacg aggcctttgc gccgatctac gtgaagctgg
     7021 cagatggcac ccgcaacgac gcctgagacc tggttgcgca tcgtcgaggc ggcgctgctc
     7081 tccgcgccgg gggcactgcc ggtgtcggag ctgcgcaagc tcttcgacga ggatccgggg
     7141 ccggacctgg tgcgccgcct gctcgacgag ctgcgcgcgc agtggttcga ggccgggcgc
     7201 ggggtcgagc tggtgcagtt ggcgagcggc tggcgcttcc agacacggcc cgagtatcag
     7261 gtctacctcg accgcctgaa ggaagaaaaa ccgccgcgct actcgcgcgc ggtcatggaa
     7321 acgctggcga tcatcgccta tcgccagccc gtcacgcgcg gtgacatcga ggacatccgc
     7381 ggcgtggcgg tctcgcccag cgtcttgaaa accctcgaat cgcgcggctg ggtcgacatc
     7441 gtcggccacc gcgacacgcc cgggcggccg gcgctcttcg ccaccacccg gcgcttcctc
     7501 gacgagatgg ggctgcgcag cctgaccgag ctgccggcgc tcaccgaact cgaaaaaatc
     7561 atggatctag tcgatgtctc gcaaaccgaa ccggccgctg cgcccgcccc tgaagaagaa
     7621 gtctgacgcg atcgagatcc tcgaccgcga cccgcgcgaa tcccgtggcg cgcgcgacga
     7681 cgacgcccag cccggccgca aggacgcgca cctcaacgcc gatccgcgcg gcccgcgcac
     7741 gccgcgtccg ccgcgcgccg gtggcgcgcc tgcgccgcgc cggccgcgca tcgagggcga
     7801 tcccgtccgc catcccgacg ctcaccgttc cgcgctcggc cagcccgagg gcggcgagcg
     7861 ccccgtgcgt ggccgtgggc cgggcggcgg gcgcaacgcg cccaccacgc tcagcgagcc
     7921 cgagcgcctg cagaaggtgc tcgcccaggc gggcgtcgcc tcgcggcgcg agatcgagga
     7981 atgggtggtg gccgggcgca tctcggtcaa tggcctgccg gcctcgctcg gccagaagat
     8041 cggcccgggc gaccgcgtca aggtcaatgg caagctcgtg ccgctgcgct tcacccagcg
     8101 ctcgccgcgc gtgctgatct accacaagcc cgaaggcgag atcgtctcgc gcgacgaccc
     8161 cgagggccgt cccaccgtat tcgagcgcct gcccatcctg cgcaaggggc gctggctggc
     8221 ggtcgggcgc ctggacttca acacctcggg gctgctgttg ttcaccaacg acggcgacct
     8281 cgccaacaag ctgatgcacc cgcgctacga actcgagcgc gagtacgcgg tacgcatcct
     8341 cggcgagctc accgaagagc aggtgaagtc gcttaccgag ggcatccagc tcgaggacgg
     8401 cccggccaag ttcaacctgc tgcgcgacga gggcggcgag ggtgccaacc actggtaccg
     8461 ggtgacgatc tcggaaggcc gcaaccgcga agtacggcgc atgttcgaag ccgtgggcct
     8521 gaccgtgagc cggctgatgc gggtgcgcta cggcagcgtc gagctgcccg cgcgcctcaa
     8581 gcgcggcatg tggatggaga tgcccgaggc cgaggcctgc gcgctcgccg gcctgccgcc
     8641 gccgcagcag agccgtcagc aggatctgcg cgacaagcgg ccggtgaagc tgcaccgcac
     8701 ccaggcgcgt tgaggcgtcc cgcagggact ccgcagtcct cgccggggga gcggcgcacg
     8761 cgcgatcttc gcgcgtgcgt ttgctcgtcc ccgcggtttg gctgtatact cgtccggctt
     8821 tgatggatgg atttcctttg tggccacgag ccacggcagg cgtccgctcc gtcatgcggt
     8881 tgtggccagc ggtttttcgt gcaggaacaa ttgtttgatg ggcgtttcgc ccatttttta
     8941 tttgtggcgc ccgacgtggc gccgaagcgg tggggcaggc agatgcgcgc ggacgtcgag
     9001 ggattgatcg agcaggtggt gaccggactg ggatacgaac tcgtcgatat cgagttctcg
     9061 cccaagggcc gcttgctgcg cgtcttcctc gacatcgagc gcggcatcac ggtcgacgat
     9121 tgcgccaccg tcagcaacca gctgcagcgc gtgttcgagg tcgagaacgt cgattacgac
     9181 cggctcgagg tgtcctcgcc cggcctcgac cgcccgttga agaagctggc cgatttcgaa
     9241 cgtttcgccg gcgagcaggc gcaggtccgc ctgtcgctgc cgatcggcaa tcaacgcaat
     9301 ttcgtgggcg tgatcgagcg ggtgcaggac ggcgcggtgg cgctgcgcac cgagaagggc
     9361 gaggtgctgc tgcccttcga cgacatcgag aaagcgcgcc tggtgcccca attttgagac
     9421 tgtggatgtg gaggttttac tgaaatgagc cgcgagatcc tgctgcttgt cgatgccttg
     9481 gcgcgcgaga agaacgtcgc caaggacatc gttttttccg cgctcgaaac cgccttggcc
     9541 tctgcgacca agaaacgcat ccacgacgat gccgacgtgg tggtgtcgat cgaccgcgat
     9601 tccggcgact acacctcgaa gcgccgctgg ctggtgatgc tcgacgagga ggtcgcgaac
     9661 gacgaggccg agatgggcat catcgatgcc cgcgagctgc gcgccgacgt gcagatcggt
     9721 gactacatcg aggaagagct cgagccgatc gacttcggtc gcatcggcgc ccaggccgcc
     9781 aagcaggtca tcctgcagaa gatccgcgac gccgagcgcg agcaggtgct caacgacttc
     9841 ctcgaccgca aggagttcct cgtctccggc tcgatcaagc gcatggagcg cggcaacgcg
     9901 atcatcgagg tcggccgcat ggaagccgtg ctgccgcgcg accagcagat cccgcgcgag
     9961 aatctgcgcg tgggcgatcg tgtcaaggcc ttcctgctgc gcatcgaccg cggcgcgcgc
    10021 ggcccgcagc tggtgctgtc gcgcaccgcg cccgaattcc tcatgaagct cttcgagctc
    10081 gaggtccccg agatcgagga cggcctgctc gagctcaagg cctgcgcccg cgacgccggc
    10141 ctgcgcgcca agatcgcggt caagtccaac gaccaacgca tcgacccgat cggtacctgc
    10201 gtcggcctgc gcggctcgcg cgtcaccgcc gtgcgcaacg agatcgccgg cgagcagatc
    10261 gacatcatcg tgtggtcgca ggatcccgcc cagttcgtgg tcgccgcgct gcagcccgcc
    10321 gaggtcgtct ccatcgtcgt ggacgaggag tcgcacgcga tggacgtggt ggtcgacgag
    10381 aacaacctcg cgatcgccat cggccgcagc ggccagaacg tcaagctcgc ctccgagctc
    10441 accgggtgga cgatcaacct gatgagcgag caggagtcgg ccgaaaagac cgcccaggag
    10501 cagcagggcc tgcgcgcgct gttcatggaa aaactggacg tcgacgagga agtcgccgac
    10561 atcctgatcg aggagggttt ctcctcgctc gaagaggtgg cctacgtgcc gctctccgaa
    10621 atgctcgaga tcgaggcctt cgacgaggac acggtcaacg aactgcgcaa tcgagcgcgc
    10681 aatgtgctgc tgaccgaggc catcgtcacc gaggagcagc tcgagaaggt ttccgacgac
    10741 ttgctcggcc tcgaaggcat ggacaagtcg ctggccgcca cactggccca gcagggcatt
    10801 cgtacccgcg acgacctggc cgaccttgcg gtcgacgagc tggtcgaaat ggccgggatc
    10861 gacgaagaaa gagccaaggc gctgatttcc gttgcgcgcg cccattggtt cgaagaatga
    10921 tgggagggca gtaatggaac agatgagcgt gacccagttt gccggcgaac tgaaaatgcc
    10981 ggccgcggtg ctgctcgagc agttgaagcg tgcgggcgtg gaaaaatccg gccccaccga
    11041 cctgctcacc gaacaggaca aggcgaggct gctggactac ctgcggcgtt cgcacggcga
    11101 cacccagccc aagggcaaga tcaccctgac gcgcaagcag acctccgaga tccgcgccac
    11161 cgactccacc gggcgtgcgc gcacggtgca ggtcgaggtg cgcaagaagc gcaccttcgt
    11221 caagcgtgac gagctcgccg agcaggccgg ggtcgaggct gcggcggtcg agcaagccga
    11281 aatcgccgag gcggtcgtcg aggcgcccgc gccggtcacc gaggccgctg cgcccgcggt
    11341 cgagcctgcc gtcgaagcag cgccggttgt tgcagccgcc gcgccggagg tggtcgagcc
    11401 gccggcggcc gaggccgtcg cgcccgtcgc cgaggccgta gctccggtcg tcgagccggc
    11461 ccccgccgtc gcgccgcccc ccgccgcccc cgccgccagc gcggcgcgac cggcccagca
    11521 gggcggtcgc catggccgtg gcgagcgtcc gcagcgccag gagcccgtcg cgccccagcc
    11581 gcaggtcacc accgtgctga gcaagccgct gccgctctcc gagatcctca gcgaggagga
    11641 ggtcgccgcg cgcaagcgtg acgaggatcg tcagcgcgcg ctcaaggagc gccaggcggc
    11701 cgacctgcgt gcccgccagg agcgcgaggc tgcggccaag gccgccgccg aggcgcgcaa
    11761 ggccgaggaa gaagcccgcc tgcgtgccga gcagcagaag aaggaagagc ccgccaaggc
    11821 ggccgccaag ccgaccaccg gcaccctgca tcggcccgcc aagaccgagg acaagcccgc
    11881 cagccgggaa gtcaagcgta cggcccccgc ggccccggcg cgcgaggccg acggcgccag
    11941 caagcgccgc ggcggcatga agacgcgcgg cgaggtcggt gccaccaccg gcagcaactg
    12001 gcgcggcgcc ggcaagggcg gcggccgtca cggccgcaac cagcaggacg accgttcgtc
    12061 cttccaggcc cccaccgagc cgatcgtgcg cgaggtgcac gtgcccgaga cgatcaccgt
    12121 cgccgacctc gcccacaaga tggcggtgaa ggcgaccgag gtgatcaagg tcctgatgaa
    12181 gatgggctcc atggtcacca tcaaccaggt gctcgaccag gaaaccgcga tgatcatcgt
    12241 cgaggagatg gggcacctgg cggtcgccgc gaagctcgac gatccggatg ccttcctcga
    12301 ggagagcgaa gcgcacaagg atgccgacgt gctgccgcgt gcgccggtgg tcaccgtgat
    12361 ggg
//
LOCUS       DAOQBS010000093        12181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4346_length_12181_cov_15.675491, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000093 DAOQBS010000000
VERSION     DAOQBS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 12181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12181
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4346_length_12181_cov_15.675491"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..174)
                     /locus_tag="PKN25_10780"
     tRNA            complement(99..174)
                     /locus_tag="PKN25_10780"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(139..141),aa:Ala,seq:cgc)
     gene            complement(490..1602)
                     /locus_tag="PKN25_10785"
     CDS             complement(490..1602)
                     /locus_tag="PKN25_10785"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNV91427.1"
                     /translation="MSTPGAGRRGRIESSTPAAIAEAAALLRAGELVGMPTETVYGLA
                     ADALDPEAVKKIFAAKGRPADHPLIVHLPSVEHLPQWAAAIPKDALALARAFWPGPLT
                     LILKRTPDVPDEVTGGQDTVGVRVPAHPVALALLRAFDGGLAAPSANRFGRISPTTAA
                     HVHEELGERVAMILDGGACAVGIESTILDFSRDVPEILRPGAITPEDIARVIGRRPRV
                     PGESVAETASARAGQCGDASGLAEAAAPNAAADAAQPAPRVSGALAAHYAPRTPLRLV
                     EPALLADEAAALAGEGSRVAVLAHSTSDPRDARLSWRSLPADPAAYAQGLYASLRALD
                     GVGADLILIEALPGGPGWRAVADRLGRAAVGSGATD"
     gene            complement(1613..2779)
                     /locus_tag="PKN25_10790"
     CDS             complement(1613..2779)
                     /locus_tag="PKN25_10790"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNV91428.1"
                     /translation="MILPPATLGMLGGGQLGRFFVSAAHEMGYKVWVLDPDPHSPAGL
                     VADRHLVAAYDDYVALDSLGNECAAVTTEFENVPADTLDYLTKFIPVHPAASAVAVCQ
                     NRIAEKTFLADNGLPHGPFAVVRSEDDVRAANAGLFPAILKVARFGYDGKGQSRVADR
                     DEALAAFQHFKGEPCVLEKMLALDYEVSVVLARDEAGNVRAFPTGENRHRNGILDVTI
                     APARASACPSEGTQPSAQEIAERIADKLGYVGTLGVEFFVCRGELVVNEMAPRPHNSG
                     HHTIDACDASQYEQQVRALCGLPLATPRQHSAAVMVNLLGELWYEDGKGHGAYREPDW
                     SVLHAVPGLRLHLYGKHHARPGRKMGHFVVIGDDAAVVLERALAARAAIGIRDE"
     gene            complement(2790..3281)
                     /gene="purE"
                     /locus_tag="PKN25_10795"
     CDS             complement(2790..3281)
                     /gene="purE"
                     /locus_tag="PKN25_10795"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNV91429.1"
                     /translation="MNETQPVVGIIMGSNSDWPTMKAAAKMLEDFGVPFEAKVVSAHR
                     TPDLMFEYAEAARSRGLKAIIAGAGGAAHLPGMVAAKTTLPVLGVPVQSKALSGQDSL
                     LSIVQMPKGIPVATFAIGEAGAANAGLFAVALLAVEDAVLAHKLADFRARQTQAVLDM
                     TLD"
     gene            complement(3292..4179)
                     /gene="folD"
                     /locus_tag="PKN25_10800"
     CDS             complement(3292..4179)
                     /gene="folD"
                     /locus_tag="PKN25_10800"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNV91430.1"
                     /translation="MTARIIDGNALSARVRGEIAERAAALTARGVQPCLAVMLVGADP
                     ASAVYVRNKVAGCEKAGIRSLRFDYPETATQAEVMAKLAELNADPAVHGILVQLPLPK
                     HLDETAVLEAIDLKKDVDGFHAENVGRLSQCREAFIPCTPHGVMKMLESSGTPVQGAE
                     AVVIGRSNIVGKPMATLLTNAGATVTVCHSRTKDLAFHTRRADILVAAIGKPRFVTGD
                     MIKPGATVIDVGINRLQDGPDAGKLCGDVDFESAREVAGAITPVPGGVGPMTITMLLE
                     NTVISAERAASDRKLAAGK"
     gene            complement(4280..6022)
                     /locus_tag="PKN25_10805"
     CDS             complement(4280..6022)
                     /locus_tag="PKN25_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNV91431.1"
                     /translation="MTGINALFLLTGLLLFISVLASTISARLGLPLLLLFLGVGMLAG
                     EDGPGGIVFEDFFIASLIAQAALSVILLDGGLRTRVSSFRVALRPAAVLASWGVIGTA
                     ALLGVFATWLLDVDWRLGMLLAAIVGSTDAAAVFALLRNSGVRLNERVQATLEIESGA
                     NDPMAILLVTAMVGTLLQPETATLGSFVWMLASQLGLGAILGLAGGWVLSRLMMRLAL
                     PDGLYALLILSGGTLVFAATNLLNGSGFLAVYLAGIVVGNRRSHATEHVLRVMDGLAW
                     LAQAGMFVVLGLLVTPSKMIDHAAEALAMAVFLMLVARPLAVAIGLLPFRYPRNELLY
                     VSWVGLRGAVPVVLAIVPVVMGVPDSQLLFNVAFAVVLLSLLVQGATVPLAARWLGVE
                     VPPRDEPRDKREVWVGEAAAVDMLEFCVAAGAKADGRHPDELGTDHPGHDVRCVALVR
                     EGSLHRLTPDTRLRPGDSVWMIAPDEISEQLARTFAGSRGELTANASFFGEFVVDPDS
                     RASDLADAYGLRIAEAERELAMGELLAVRLGRPPVVGDRVDIGAFALTVREMGDKGRI
                     TALGLKCPPFGGGR"
     gene            complement(6168..6806)
                     /locus_tag="PKN25_10810"
     CDS             complement(6168..6806)
                     /locus_tag="PKN25_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV91432.1"
                     /translation="MSAVPAAPDQIVHVVDDDEALRDSLAWMLEANGHAVAVHESGEA
                     FLAACTPEMTGCVLLDVRMPGMSGLEVFEELGRRRCGLPVVFITGHGDVPMAVSALKQ
                     GAVDFVEKPFGEREMLRVVEQCLRLERETRDRRLQEADTARRLGLLTQREREVLELII
                     VGKLNKQIADVLGISIKTVEVHRARVMEKMGAHSLAELVQHVVVLEPGAVPR"
     gene            complement(6781..9489)
                     /locus_tag="PKN25_10815"
     CDS             complement(6781..9489)
                     /locus_tag="PKN25_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV91433.1"
                     /translation="MQASAAARATLSRWYWAMPYLAVTVLALSMLAVVWLLQARETAV
                     ERDALARDLQWTEQSIRRAMLTTEEFLVQLARDLSAGTLDHDDFQLRANEHLAINPAL
                     ANLAWVDREHVIAWSAPFDTRDLLAGEILVDDQVEAFQNSALSARLTYGRPYIDARGS
                     AVIEVYAPVLRRGDSLGAIVAVYSIERMLSRLVPARFAEKYRLAVLDRDGGVLTLSSA
                     LPPAQEALSLTLALDPPGNGLALQAMAFRPGGAVLRYLPAVLIVGLSLLVLWSLWTLR
                     RHVQRRVRVEKERDQLFDLSLDLLCVIDLDGRFGRCNPAFERVLGHDPRSLPGCSLID
                     FVHPEDVSETLAMLRRLAGGEPVRFETRCRCADGSYKWLMWSINPVREERLVYAVAHD
                     VTGRKATEDALRAESAFRKAMEDSVVTGLRAIDLSGRIIYVNTAFCRMIGFDEDELVG
                     AGPPFPYWPPEQLAECERNLSMTLSGRAPRSGFEMRILRKNGERFDARFYLSPLIDLT
                     GRQTGWMASITDITEPKRVRAALESAHERFEAVLDGLDAAVFVADARTDEILFANLAF
                     KRIHGFDAVGRTVRGVAVPQPERGDYRVDPRNLSPADVPRELFDGELLHPQSGRWYHV
                     REQATRWVDGRVVRMGIATDITDRKQTAAVAREQEERLQRTSRLITMGEMASTLAHEL
                     NQPLAAIANYCAGSVTRLQSGKARTEDVLAAMQKASFQAERAGKIIRRVREFVKKSEP
                     QRCAVDLVEVLEDAIGFADIDAHRTRIRIHTELEPGLPPVYADRIMIEQVLLNLIRNG
                     LDAMADALPEARVLTVRVRTVGADAVEVAVIDRGHGISEEGRAQLFTPFYTTKAEGMG
                     MGLNICRSIVEFHNGRLLVDANPEGGTIFTFTLPTESAIERSARSA"
     gene            9679..10926
                     /locus_tag="PKN25_10820"
     CDS             9679..10926
                     /locus_tag="PKN25_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV91434.1"
                     /translation="MKPFLALLGRAFVTLLLLAPQLSAQAVPAEAVGAGARPSGGERA
                     PIRVGVSGPFSGPSAPMGLSMREGIRIAAEELNAGGGLLGRRIELVERDDEASNELGA
                     QIVRDFIHRERVTAGLGIVNTGVALASQRHYQMARIPVITSVATGSLITKQFQPPDFT
                     ENYVFRVSASDTLQAAVIVEEAVGRRGLTRLAILHDATNYGVLGSQDLIAALGTRGRT
                     AVVVERFQLRETDMRPQLERARAAGAQAVLTYGIGPELAHIANSMARLGWQVPIIGSW
                     TLAMSSFIELAGRNAEGARMPQTFIAEARSPAQAAFLAAWERATGSVRIPVPPAAAQG
                     YDSMQLLAAAIRQAGSLDGPRIREALENLDAEVDGVIMRYRRPFSRDNHETLRSARQI
                     HLGEIRDGAVVFAHEAAPERPGP"
     gene            11110..>12181
                     /gene="aceE"
                     /locus_tag="PKN25_10825"
     CDS             11110..>12181
                     /gene="aceE"
                     /locus_tag="PKN25_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936832.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNV91435.1"
                     /translation="MTGISTVLPQTDTDAQETKEWLDALAAVVEHEGPERAHYLIETL
                     IATARQEGVNIPYSATTEYINTIPAERQPPYPGDPDLEIKIHSYIRWNAMAMVVRANK
                     DTNVGGHIASFASAAALYDVGFSHFWHAPSEAHDGDLIYFQGHSVPGVYARAFMLGRL
                     TEEQMDSFRQEVGGKGISSYPHPWLMPDFWQFPTVSMGLGPLCAIYSARFMKYLASRG
                     LIDPARAQQRKVWAFLGDGETDEVESLGAIGMAGREGLDNLVFVINCNLQRLDGPVRG
                     NGKIIQELESEFRGAGWNVIKVIWGTHWDALFARDKKGILKQRMMELCDGEYQTFKAK
                     DGAYVREHFFNTPELRALVADWT"
BASE COUNT         1919 a         4187 c         4334 g         1741 t
ORIGIN      
        1 cacaactttg ccacactgac tcagcaaaaa cctgccatac aaagcaaaac agcgcatcac
       61 actgcaaagt gtaatgcgct gtttttgcta ggaattcttg gtggaccgaa ggaggatcga
      121 actcccgacc tctgcattgc gaacgcagcg ctctcccagc tgagctatcg gccctgaaag
      181 agcgcgcatt atagtgagac cccgcgcgaa agggaagtgc cttgcgcaca tttgcgccgt
      241 tcgaggctac gtgtgccctt tgcaggagcg acgcgagtcg cgatccggcg gtggggcggt
      301 gatgaggcgt cggtgaaggc tcggaggccg tcatcgcgac ttgcgtcgct cctacgtgtg
      361 ccctttgcag gagcgacgcg agtcgcgatc cggcggtggg gcggtgatga ggcgtcggtg
      421 aaggctcgga ggccgtcatc gcgacttgcg tcgctcctgc ggggcaggta gcgcgagggc
      481 gcggcagggc tagtcagttg cgccggagcc gacggcggcg cggccgaggc ggtcggcgac
      541 ggcgcgccag ccggggccgc cggggagcgc ttcgatcagg attaagtcgg cgccgacgcc
      601 gtcgagggcg cgcaggctgg cgtacaggcc ctgggcgtag gcggcgggat cggccggcag
      661 gctgcgccag ctcaggcggg cgtcacgcgg atcggaggtt gagtgggcga gcactgcgac
      721 gcggctgccc tcgcccgcca gcgcggcggc ctcgtcggcg agcagcgcgg gctcgaccag
      781 gcgcagcggg gtgcgcgggg cgtaatgggc ggcgagtgcg ccggagacgc gcggagcggg
      841 ctgcgctgca tcggctgcgg cgttgggggc ggcggcttcc gcgagtccgg acgcgtcgcc
      901 gcactgccct gcccgcgcgg aagcggtttc cgcgaccgac tcgcccggca cccgcggccg
      961 gcggccgatg acgcgcgcga tgtcctcggg cgtgatcgcg cccgggcgca ggatctccgg
     1021 cacgtcgcgc gagaagtcga ggatggtgga ttcgatgccg accgcgcagg caccgccgtc
     1081 gaggatcatc gccacgcgct cgccgagttc ttcgtgcaca tgcgcggcgg tggtggggct
     1141 gatacggccg aagcggttgg cgctgggcgc ggccagcccg ccgtcgaagg cccgcagcag
     1201 ggcgagcgcg accgggtgcg cgggcacgcg cacgccgacc gtgtcctgcc cgccggtgac
     1261 ttcgtcgggt acgtcgggtg tgcgcttcag gatcagcgtc agcgggccgg gccagaaggc
     1321 gcgcgcgagc gcgagcgcgt ccttggggat ggcggcggcc cactgcggca ggtgctcgac
     1381 cgagggcagg tgcacgatca gcgggtggtc ggcgggacgg cccttggcgg cgaagatttt
     1441 cttcaccgcc tcggggtcga gcgcgtcggc ggccagcccg tagaccgtct cggtcggcat
     1501 gccgacgagt tcgcccgcac gcagcagggc agcagcctcg gcgatcgcgg cgggcgtgga
     1561 ggattcgatg cggccgcgcc gtccggcccc gggggtactc atcggccggc gctcactcgt
     1621 cgcggatgcc gatcgcggca cgcgcggcga gcgcgcgctc gagcaccacg gcggcatcgt
     1681 cgccgatcac cacgaaatgg cccatcttgc gccccgggcg ggcgtggtgc ttgccgtaga
     1741 gatgcaggcg caggccgggc accgcgtgca gcaccgacca gtcgggctcg cggtaggcac
     1801 catggccctt gccatcctcg taccacagct cgccaagcag attcaccatc accgccgccg
     1861 aatgctggcg cggggtggcc agcggcaggc cgcacagcgc cctcacctgc tgctcgtact
     1921 ggctggcgtc gcaggcgtcg atggtgtggt ggccgctgtt gtgcgggcgc ggggccatct
     1981 cgttcaccac cagctcgccg cggcagacga agaactccac gcccagcgtg ccgacgtagc
     2041 cgagcttgtc ggcgatgcgc tcggcgatct cctgcgcgct cggttgcgta ccctcactgg
     2101 gacaggccga cgcgcgcgcc ggcgcgatgg tcacgtcgag gatgccgttg cggtggcggt
     2161 tctcgccggt cgggaaggcg cgcacattgc ccgcctcgtc gcgcgccagc accaccgaca
     2221 cctcgtagtc cagcgccagc atcttctcca gcacgcaggg ctcgcccttg aagtgctgga
     2281 aggcggcgag cgcctcgtcg cggtcggcga cgcgggactg gcccttgccg tcgtagccga
     2341 agcgggccac cttgaggatg gcggggaaca gcccggcatt ggctgcgcgc acgtcgtcct
     2401 cgctgcggac gaccgcgaag gggccgtgcg gcaggccgtt gtcggcgagg aaggtctttt
     2461 cggcgatgcg gttctggcac accgcgaccg cgctcgccgc cgggtgtacc gggatgaact
     2521 tggtcaggta gtcgagcgtg tcggcgggga cgttctcgaa ctcggtggtc accgcagcgc
     2581 attcgttgcc caggctgtcg agggcgacgt agtcgtcgta ggcggcgacc aggtggcggt
     2641 cggcgacgag gccggcgggg ctgtgcggat cggggtcgag cacccagacc ttgtagccca
     2701 tctcgtgcgc ggccgaaacg aaaaagcggc cgagctggcc gccgccgagc atgcccaggg
     2761 tggcaggcgg gaggatcatg atgcgacgct caatccaggg tcatgtcgag caccgcctgg
     2821 gtctggcggg cacggaagtc ggcgagcttg tgcgccagca cggcatcctc gaccgcgagc
     2881 agcgccaccg cgaagaggcc cgcgttggcc gcgcccgcct cgccgatggc gaaggtggcg
     2941 accgggatgc ccttgggcat ctgcacgatg gagagcaacg agtcctgccc cgacagcgcc
     3001 ttcgactgca ccggcacacc gagcaccggc agcgtggtct tggccgcgac catgcccggc
     3061 aggtgggccg caccgccggc gcccgcgatg atcgccttga gcccgcggct gcgcgcggcc
     3121 tcggcgtact cgaacatcag gtcgggggtg cggtgggcgg agacgacctt ggcctcgaag
     3181 ggcacgccga agtcttccag catcttcgcg gccgccttca tcgtgggcca gtcggagttg
     3241 gagcccatga tgatgccgac gacgggttgg gtttcgttca tctcgcagtc ctcacttgcc
     3301 ggccgcgagc ttgcgatcgc tcgcggcgcg ttcggcggaa atcacggtgt tttccagcag
     3361 catggtgatg gtcatcggcc ccacgccgcc cggcaccggg gtgatcgcgc cggcgacttc
     3421 cctggccgac tcgaaatcca cgtcgccaca cagcttgccg gcgtcggggc cgtcctggag
     3481 gcgattgatg ccgacgtcga tcacggtggc gcccggcttg atcatgtcgc cggtgacgaa
     3541 gcgcggcttg ccgatcgccg ccaccaggat gtcggcgcgg cgggtgtgga aggcgaggtc
     3601 cttcgtcctg gagtggcaca cggtgacggt ggcgccggcg ttggtcagca gcgtggccat
     3661 cggcttgccg acgatgttcg agcgcccgat caccaccgcc tcggccccct gcaccggcgt
     3721 gccgctggac tcgagcatct tcatgacacc gtgcggcgtg cacgggatga aggcctcgcg
     3781 gcactgcgac aggcggccga cgttctcggc atggaaaccg tcgacatcct tcttgaggtc
     3841 gatcgcctcg agcaccgcgg tctcgtcgag gtgcttcggc agcggcagct gcaccaggat
     3901 gccatgcacc gcggggtcgg cgttgagctc ggcgagcttg gccatgacct cggcctgggt
     3961 ggcggtctcg gggtagtcga agcgcagcga gcggatgccg gccttctcgc agccggcgac
     4021 cttgttgcgc acgtagaccg ccgaggccgg gtcggcgccc accagcatca ccgccaggca
     4081 gggttgcacg ccacgcgccg tgagcgcggc cgcacgttcg gcgatttcgc cgcgcacgcg
     4141 cgcggacagc gcgttgccgt cgatgatgcg agcggtcatc cttcaagccc ccgaaaaaaa
     4201 ccgcgatttt acagcatcgc ggcggcggcg gcccgtacgg cggattcggg tcggcatcgt
     4261 gcggccaatg cggcccgcct caacgccctc cgccgaacgg cgggcacttg aggccgaggg
     4321 cggtgatgcg gcccttgtcg cccatctcgc gcaccgtgag cgcgaaggca ccgatgtcga
     4381 cgcggtcgcc gaccactggc gggcggccga ggcggacggc gagcaactcg cccatcgcca
     4441 gctcgcgctc ggcctccgcg atgcgcaggc cgtaggcgtc ggcgaggtcg ctcgcacggc
     4501 tgtcggggtc gaccacgaac tcgccgaaga agctggcgtt ggcggtgagt tcgccgcgac
     4561 tgccggcgaa agtgcgcgcg agctgttcgg agatttcgtc gggcgcgatc atccacaccg
     4621 agtcgccggg acgcaggcgg gtgtcggggg tcaggcggtg caggctgccc tcgcgcacca
     4681 aggccacgca gcgcacgtcg tggccgggat ggtcggtgcc gagctcgtcg ggatggcgcc
     4741 cgtcggcctt ggcgccggcc gcgacgcaga actccagcat gtccaccgcc gccgcctcgc
     4801 ccacccacac ctcgcgcttg tcacgcggct cgtcgcgcgg cggcacctcg acacccagcc
     4861 agcgcgccgc cagcggcacg gtcgcgccct gaaccagcaa ggacagcagc accaccgcga
     4921 aggcgacgtt gaacagcagt tgcgaatcgg gcacgcccat caccaccggc acgatcgcga
     4981 gcaccaccgg caccgcgccg cgcaggccca cccagctcac gtagaggagc tcgttgcgcg
     5041 gatagcggaa gggcagcagc ccgatcgcca ccgccagcgg ccgcgccacc agcatcagga
     5101 acaccgccat cgccagcgcc tcggcggcat gatcgatcat cttcgacggt gtcaccagca
     5161 gccccagcac gacgaacatg cccgcctggg cgagccaggc caggccgtcc atcacccgca
     5221 ggacgtgctc ggtggcatgg ctgcggcggt tgccgacgac gatgcctgcc aggtacacgg
     5281 cgagaaagcc gctgccgttc agcaggttgg tcgccgcgaa aaccaaggtg ccgccggaca
     5341 ggatcagcaa cgcatacagc ccgtcgggca gcgccagccg catcatcagc cgcgacagca
     5401 cccagccccc ggcaaggccg aggatggcgc cgagtccgag ctggctcgcc agcatccaga
     5461 cgaaactgcc gagcgtggcg gtctcgggct gcagcagcgt gccgaccatg gcggtaacga
     5521 gcaggatcgc catcggatcg ttggcaccgg actcgatctc cagcgtggcc tggacgcgct
     5581 cgttgaggcg gacgccgctg ttgcgcagga gcgcgaacac cgcggccgca tcggtcgagc
     5641 cgacgatcgc cgcgagcagc atgcccagcc gccagtcgac atcgaggagc caggtggcga
     5701 acacgccgag cagggccgcc gtaccgatca cgccccagct ggccagcacc gcggccggcc
     5761 tcagcgccac ccggaagctg ctcacccgcg tgcgcaggcc gccgtcgagc aggatcaccg
     5821 agagcgccgc ctgcgcgatc agcgaggcga tgaagaagtc ctcgaagacg atgccgcccg
     5881 gcccgtcctc gccggcgagc atgccgacgc cgagaaagag cagcagcagc ggcaagccga
     5941 gccgggcgga gatggtgctg gcgagcacgc tgatgaacag cagcaggccg gtgagcagga
     6001 agagcgcgtt gattccggtc atgcggcggc ggggtccagc cggttggaag gggctttcgg
     6061 caaaacgggg cgatcgcaca cggcacaccc ccacagcgga gccgcccggg tggaagaaag
     6121 ttcctgcgca tgcgggcgcg gagatcgccc gtcggcacgc gccggggtca gcgcggaacg
     6181 gcgccgggtt cgagtaccac cacgtgctgg acgagttcgg cgagcgaatg cgcgcccatc
     6241 ttctccatca cgcgcgcgcg atgcacctcg acggtcttga tgctgatgcc gagcacgtcg
     6301 gcgatctgct tgttgagctt gccgacgatg atcagctcga gcacctcgcg ctcgcgctgg
     6361 gtgagcagcc cgagccggcg cgcggtgtcg gcctcctgca ggcggcggtc gcgggtctcg
     6421 cgctcgaggc gcaggcactg ctcgaccacg cgcagcatct cgcgctcgcc gaagggtttc
     6481 tcgacgaaat ccaccgcgcc ctgcttgagc gcggacaccg ccatcggcac gtcgccgtgg
     6541 ccggtgatga acaccaccgg cagcccgcag cgccgccgcc cgagctcctc gaacacctcc
     6601 agcccgctca tgcccggcat gcgcacgtcg agcagcacgc agccggtcat ctccggcgtg
     6661 caggcggcga ggaaggcctc gcccgactcg tgcaccgcga ccgcgtggcc gttggcctcc
     6721 agcatccagg cgagcgagtc gcgcagggct tcgtcgtcgt cgacgacatg tacgatctgg
     6781 tcaggcgctg cgggcactgc gctcaattgc ggactccgtc ggcagggtaa acgtgaatat
     6841 ggtaccgcct tcggggttgg cgtcgaccag caatctgccg ttgtggaact cgacgatcga
     6901 gcggcagatg ttgagcccca tgcccatgcc ctcggccttg gtggtgtaga agggcgtgaa
     6961 cagctgcgcc ctgccctcct cgctgatgcc gtggccgcgg tcgatcaccg cgacctcgac
     7021 cgcgtccgcg ccgacggtgc gcacccgcac cgtcagcacg cgcgcctcgg gcaaggcgtc
     7081 ggccatggcg tcgaggccgt tgcggatcag gttgagcagc acctgctcga tcatgatgcg
     7141 gtcggcgtac accggcggca gcccgggctc gagctccgtg tggatgcgga tgcgggtgcg
     7201 gtgggcgtcg atgtcggcga agccgatcgc gtcctccagc acctcgacga ggtcgaccgc
     7261 gcagcgctgc ggctcgctct tcttcacgaa ctcgcgcacg cggcggatga tcttgccggc
     7321 gcgctcggcc tggaagctcg ccttctgcat cgccgcgagc acgtcctcgg tcctcgcctt
     7381 gcccgactgc agccgcgtca ccgaaccggc gcagtagttc gcgatcgccg ccagcggctg
     7441 gttgagctcg tgcgccagcg tcgaggccat ctcgcccatg gtgatcaggc gcgaagtgcg
     7501 ctgcaggcgc tcctcctgct cgcgcgccac cgcggcggtc tgcttgcggt cggtgatgtc
     7561 ggtggcgatc cccatgcgca ccacgcgccc gtccacccag cgtgtggcct gctcgcgcac
     7621 gtggtaccag cgccccgact gcgggtgcag caattcgccg tcgaagagct cgcgcggcac
     7681 gtcggcgggc gacaggttgc gcggatcgac gcggtagtcg ccgcgctcgg gctgcggcac
     7741 cgccaccccg cgcacggtgc gcccgaccgc gtcgaaacca tggatgcgct tgaaggcgag
     7801 gttggcgaag aggatctcgt cggtgcgcgc gtcggccacg aacaccgcgg cgtcgaggcc
     7861 gtcgagcacc gcctcgaagc gctcgtgcgc cgactcgagc gcggcgcgca cgcgcttggg
     7921 ctcggtgatg tcggtgatcg aggccatcca gccggtctgc cggccggtga ggtcgatcag
     7981 cggggaaagg tagaagcgcg catcgaagcg ctcgccgttc ttgcgcagga tgcgcatctc
     8041 gaagccgctg cgcggcgcgc gccccgacag ggtcatcgac aggttgcgct cgcattcggc
     8101 gagctgctcg ggtggccaat agggaaaggg cggcccggcg ccgacgagct cgtcctcgtc
     8161 gaagccgatc atgcggcaga aggcggtgtt cacatagatg atgcgtcccg acaggtcgat
     8221 cgcgcgcagg ccggtgacga ccgagtcctc catcgccttg cggaaggcgg actcggcgcg
     8281 cagcgcgtcc tcggtggcct tgcgtccggt gacgtcgtgc gccacggcgt agaccagacg
     8341 ctcctcgcgt accgggttga tgctccacat cagccatttg tagctgccgt cggcacagcg
     8401 gcagcgcgtc tcgaagcgca ccggttcgcc accggcaagg cggcgcaaca tcgccagcgt
     8461 ctcggaaacg tcctcgggat gcacgaagtc gatcagcgag cacccgggca ggctccgggg
     8521 gtcgtgcccg agcacgcgct cgaaggccgg gttgcagcgc ccgaaacgac catcgagatc
     8581 gatgacgcac aagaggtcga gcgaaaggtc gaagagctgg tcgcgctcct tctccacccg
     8641 cacccggcgc tgcacgtggc ggcgcagcgt ccacaggctc cacagcacca gcagcgacag
     8701 ccccacgatg agcaccgccg gcaggtagcg cagcaccgcc ccaccgggcc ggaaggccat
     8761 cgcctgcagc gcgaggccgt tgcccggcgg atcgagcgcg agggtaagcg agagcgcctc
     8821 ctgcgcgggc ggcagggccg agctgagggt cagcacgccg ccatcgcggt cgagcaccgc
     8881 aagccggtat ttttcggcaa agcgcgccgg caccagccgg ctcagcatgc gctcgatcga
     8941 atacacggcg acgatcgcac cgagcgaatc gccgcgccgc agcacgggcg cgtacacctc
     9001 gatcaccgcg ctgccgcgcg cgtcgatgta gggccggccg taggtcaggc gggcggacag
     9061 ggcgctgttc tggaaggcct ccacctggtc atccacgagg atctcgccgg cgagcaggtc
     9121 acgggtgtcg aagggcgccg accaggcgat cacgtgctcg cggtccaccc aggcgaggtt
     9181 ggcgagcgcg gggttgatcg ccagatgctc gttggcgcgc agctggaagt cgtcgtggtc
     9241 gagcgtgccc gccgacaggt cgcgcgcgag ctggacgaga aactcctcgg tggtgagcat
     9301 ggcgcgccgg atcgactgct cggtccactg caggtcgcgc gcgagcgcgt cgcgctcgac
     9361 cgcggtctcg cgcgcctgca gcagccagac gaccgcgagc atcgacagcg cgagcacggt
     9421 caccgcgaga tagggcatgg cccagtacca gcgcgacagg gtcgcccggg cggcggcgga
     9481 ggcttgcatg gaggatggcc ggacgagcgg cgcggggagg cggatcggga gcaggattgt
     9541 tgtcgagcgc gaccgcccgc gtcatccggt gtaaccctga tcgggggaca catacccggt
     9601 gtggatgcgg cagcgcagca gacggcgatg gggtttccgc caatttgtgc gcttgttcac
     9661 gctgcctaca gtgcaaggat gaaacccttc ctcgcgctgc tcgggcgcgc cttcgtgacg
     9721 cttctgctcc tggcgccgca gttgtcggca caggccgtcc cggcagaggc cgtgggcgcg
     9781 ggcgcacgcc cgagcggcgg cgagcgggca ccgatccggg tgggcgtgtc ggggcccttc
     9841 tccggtccgt cggcgccgat ggggctgtcg atgcgcgagg gcatccgcat cgccgccgag
     9901 gagctcaacg ccggcggcgg cctgctcggt cggcgcatcg agctcgtcga gcgcgacgac
     9961 gaggcgagca acgagcttgg cgcgcagatc gtgcgcgact tcatccatcg cgaacgtgtc
    10021 accgccggac tgggcatcgt caataccggt gtcgcgctcg ccagccagcg ccattaccag
    10081 atggcgcgca tcccggtgat cacctcggtc gccaccggct cgctgatcac caagcagttc
    10141 cagccccccg atttcaccga gaactacgtg ttccgcgtct ccgccagcga caccttgcag
    10201 gccgcggtga tcgtcgagga ggctgtcggc cggcgcggcc tgacccggct cgcgatcctg
    10261 cacgacgcca ccaactacgg ggtgctggga agccaggatc tcatcgcagc cctgggcacg
    10321 cgcgggcgga ccgcggtggt ggtcgagcgc ttccagttgc gcgagaccga catgcgaccg
    10381 cagctcgaac gcgcacgcgc cgccggtgcg caagccgtgc tcacctatgg catcggcccc
    10441 gagctcgccc acatcgccaa ctcgatggcc cgcctcggat ggcaggtgcc gatcatcggc
    10501 agctggacgc tggccatgtc gagcttcatc gaactcgccg gccgcaacgc cgagggcgca
    10561 cgcatgccac agaccttcat cgccgaggca cgcagcccgg cgcaggcggc cttcctcgcg
    10621 gcgtgggagc gcgccaccgg cagcgtgcgt atcccggtgc caccggcggc cgcacagggc
    10681 tacgactcga tgcagctgct cgccgcggcg atccgccagg ccggcagcct cgacggtccg
    10741 cgcatccgcg aggcgctcga aaacctggat gcggaggtcg atggcgtgat catgcgctat
    10801 cgccgcccct tctcccgcga caaccacgaa accctgcgca gcgcgcgcca gatccacctc
    10861 ggcgagatcc gcgacggcgc cgtggtgttc gcccacgagg cggcgccgga gaggccggga
    10921 ccatgagcgc gagcgatcag ggagccgatt tcgtgttcac caggtgaaaa catattgcac
    10981 attgcgaaaa gacgttttat attttgaaac gctgaccgtg cggcagccgg catggtcgtg
    11041 cccgtaacca acaacccgcc tgtcccgcca gacaggaatc gccaactgaa cgtcatgagg
    11101 agacaaacca tgaccggaat cagcaccgtt ctcccgcaaa ccgacacgga tgcgcaggaa
    11161 acgaaggaat ggctggacgc gctcgccgcc gtcgtcgaac acgaaggccc cgagcgcgcc
    11221 cactacctga tcgagaccct gatcgccacg gcgcgccagg agggggtgaa catcccctac
    11281 tcggccacga ccgagtacat caacacgatt ccggccgagc gccagccgcc ctacccgggc
    11341 gatccggatc tggagatcaa gatccactcc tacatccgct ggaacgcgat ggcgatggtg
    11401 gtgcgggcga acaaggacac caacgtgggc ggtcacatcg cctccttcgc ctcggcggcg
    11461 gcgctctacg acgtgggctt ctcgcacttc tggcacgcgc cctccgaggc gcacgacggc
    11521 gacctgatct acttccaggg ccactcggtg ccgggcgtgt atgcgcgcgc gttcatgctg
    11581 ggccgtctga ccgaagagca gatggacagc ttccgccagg aagtgggcgg caagggcatc
    11641 tcgtcctacc cgcacccctg gctgatgccc gacttctggc agttccccac cgtgtcgatg
    11701 ggcctgggtc cgctgtgcgc gatctactcg gcgcgcttca tgaaatacct ggccagccgc
    11761 ggcctgatcg atccggcacg cgcgcagcag cgcaaggtgt gggccttcct gggcgacggc
    11821 gagaccgacg aggtcgaatc gctgggcgcg atcggcatgg ccggccgcga gggcctggac
    11881 aacctcgtct tcgtgatcaa ctgcaacctg cagcgcctcg atggtccggt gcgcggcaac
    11941 ggcaagatca tccaggagct cgagtccgag ttccgcggcg cgggctggaa cgtcatcaag
    12001 gtgatctggg gcacgcactg ggacgcgctc ttcgcccgcg acaagaaggg catcctcaag
    12061 cagcgcatga tggagttgtg cgacggcgag tatcagacct tcaaggccaa ggacggcgcc
    12121 tacgtgcgcg agcacttctt caacacgccc gagctgcgcg cgctggtggc cgactggacc
    12181 g
//
LOCUS       DAOQBS010000094        11991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4449_length_11991_cov_12.187081, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000094 DAOQBS010000000
VERSION     DAOQBS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11991
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4449_length_11991_cov_12.187081"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PKN25_10830"
     CDS             <1..692
                     /locus_tag="PKN25_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNV91436.1"
                     /translation="FTYMWAGPHHLHYTALPDWTQSVGMVFSLILLAPSWGGMINGVL
                     TLSGAWHKLRTDPILKFLITSLSFYGMSTFEGPMMSIKTVNALSHYTDWTVGHVHSGA
                     LGWVAMISIGSVYFLLPRLYGKSEMYSVKLINAHFWIATIGIVLYIASMWISGVMQGL
                     MWRATNPDGTLTYSFVESVKASYPFWTIRFIGGAMFLGGMLIMFYNMVKTIAGQKAYD
                     APVLAPAAAHA"
     gene            708..1343
                     /gene="ccoO"
                     /locus_tag="PKN25_10835"
     CDS             708..1343
                     /gene="ccoO"
                     /locus_tag="PKN25_10835"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNV91437.1"
                     /translation="MAQSKHEKIERNVFLMIVLTLMTVSVGGLVEIVPLFFQHSTTTA
                     IDHKGNALDVKPYDPVRLVGRDIYVREGCYNCHSQMIRPFRAETERYGHYSVAGEYIY
                     DHPFQWGSKRTGPDLARVGGRYSDEWQRVHLLNPRDVVPESNMPAFPWLDRPVRAADI
                     QDRMRALNKVGLHKYSDEEIAAAPAALEGKTELDAVVAYLQGLGTAISHFR"
     gene            1359..1547
                     /locus_tag="PKN25_10840"
     CDS             1359..1547
                     /locus_tag="PKN25_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HNV91438.1"
                     /translation="MDINDFRSLITVLGLLCFLGICAWAYSKQAKAGFDEAARLPLSD
                     DDLPAPRGRQDQEGKTNG"
     gene            1540..2430
                     /gene="ccoP"
                     /locus_tag="PKN25_10845"
     CDS             1540..2430
                     /gene="ccoP"
                     /locus_tag="PKN25_10845"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HNV91439.1"
                     /translation="MADFISGFWNMYVMVLVALSILFCVFVLASNMTKREKGPVQLHG
                     HVWDETLAEYNNPLPRWWMYLFWITIVFAVVYLAIYPGFGRSNQSRGQWAQYDAEMQA
                     AQERFGPVFAKYREMDVMAVAADPEANAMGKRLFLTYCQQCHGAAAQGAKSFPNLTDS
                     EWLWGGAPENIKETITNGRNGVMPPFGPALGADGVKDVANYVRSMNGLAHDSVRAQRG
                     KEAFESNCVACHGADAKGNPMLGAPNLTDNRWLYGSSEAVIIETVTNGRNNKMPAFGA
                     FLGEDKVHLLAAYVLSLSKK"
     gene            2534..3967
                     /gene="ccoG"
                     /locus_tag="PKN25_10850"
     CDS             2534..3967
                     /gene="ccoG"
                     /locus_tag="PKN25_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNV91440.1"
                     /translation="MKSASPEPQKAARPSNPESSQDTLYAARKKLYVRSVTGLFANWR
                     WALVWITQILFYGLPWLSWNDRQAVLFHLTERKFYIFGWVFWPQDVFFLAILLIISAY
                     ALFFFTAIAGRLWCGYACPQTVYTEIFMWIEEKIEGDHTRRQKLDRAPMSSGKALRRA
                     VKFLVWGIVALWTGFTFVAYFSPLDELLASFNGLTFGPWETFWILFYAGFTYLFAGVL
                     REQVCKYMCPYARFQAVMFDPDTLVITYDEARGEPRGTRKKGIDPRSVSKGDCIDCGI
                     CVQVCPTGIDIRDGLQYECIGCAACIDACDQVMDKMNYPRGLIRYSTENAMKRNWGSK
                     EILAHVFRPRTLIYGTILAAICAAFVWGLAAREPLRVDVLRDRSSLGQEIEGGLIENV
                     YQLQVMNMTESPRAFSIGVSGLNGIRIKGPQRVEVAPAGVQSVTVQVHVPYDEGKPGA
                     NTIFFDVAAEDDPALTLKEESTFLVPR"
     gene            3969..4478
                     /locus_tag="PKN25_10855"
     CDS             3969..4478
                     /locus_tag="PKN25_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNV91441.1"
                     /translation="MRSKKPEAPVEPWYRQGWPWFLIALPATAVVAGIATLVIAAKTF
                     DGMVVDDYYKEGQAIVQTLGRVEHARELGLRAALRIRSDSIHVELTATAPGSLPDSIR
                     LTVAHPTRSGLDQELLVRGRSGVYEATLAPLSTGRWLFVIQDEARSWRMDGSAYLPAE
                     MEIRMDPNV"
     gene            4503..4652
                     /locus_tag="PKN25_10860"
     CDS             4503..4652
                     /locus_tag="PKN25_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HNV91442.1"
                     /translation="MAWELLFKSDIGLLSLFTIVFILVMAVYLYRFARRHMAEDEQKA
                     RSARS"
     gene            complement(4735..5178)
                     /locus_tag="PKN25_10865"
     CDS             complement(4735..5178)
                     /locus_tag="PKN25_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV91443.1"
                     /translation="MFKHILVPTDGSALSDSTVARAVSFAKETGARITFFYSQPDFPM
                     PIYGEGALIDPTTPEQFAKSAASEAEAILSKARGAAEAAGVACDSDTAVNEVPYEAII
                     DAADRHGCDLIFMASHGRRGIAGLLLGSETQKVLTHSKTPVLVYR"
     gene            5314..6723
                     /locus_tag="PKN25_10870"
     CDS             5314..6723
                     /locus_tag="PKN25_10870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV91444.1"
                     /translation="MMLTFLGAAGEVTGSCLRVDHERGAFLVDCGLFQGGREADRKNR
                     DALDFELRDIDFVLLTHAHLDHSGLLPRLVALGYRGRIFATTATVDLLGVMLMDSAHI
                     QEKEAEWALRDTRSRKMARRAEVAPLYTVEQARTSLGRFVPTDYDDWIEPGPGVRCRF
                     RDAGHILGSAILEIDVGERGHTRRLVASGDIGQPMRPVLRDPVRIARADYLCVESTYG
                     NRAHRSFAATCDELALALRRTLEVDGGNVIIPAFAVGRTQEVLFVLADLVRAGRIARL
                     DVVVDSPMASAVTALTVRHTALWDDETRALYAWAEANSGRFRVRFVQDVEESKALNAV
                     PGGLVVISASGMCDAGRIKHHLRHNLGRRECAVVIAGFQAAGTLGRRLVDGAHQVTLF
                     GERIPVRARIHTIGGLSAHADQPALLDWLRGFGEAPRRTFVAHGEPAASAAFVEAVER
                     ELGWSRVEAARPGVPVVLE"
     gene            complement(6765..7448)
                     /locus_tag="PKN25_10875"
     CDS             complement(6765..7448)
                     /locus_tag="PKN25_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNV91445.1"
                     /translation="MPNTTPLDIPSLLRRIPLFSELSEADIQLVARYTRERQVARGEV
                     LFQRGDLPHGFYFVVSGQVKLAFSSSQGTEKVVEIIGPMQSFGEAVMFMNHPYPVFAE
                     ALSETVLVHVGQAVVSELIDQDSTFARKLLAGMAIRLHGLIQDVETYSLRSSTQRVIG
                     YLLQVADNDAPCEIALPTSKQVIASRLNLTPETLSRIFHDLSDAGLITVQGKRVSLHD
                     PVRLSRYNA"
     gene            7531..7911
                     /locus_tag="PKN25_10880"
     CDS             7531..7911
                     /locus_tag="PKN25_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HNV91446.1"
                     /translation="MYYLIKNLHVTCVVLSAAGFLLRGTWMLTGNALLQHRLTRLLPH
                     LVDSTLLLSAITLAVMIGQYPFSAGWVTAKVIGLLAYILLGTLALKRGRTRGVRLAAF
                     AGAILVYAWIVSVALSKNVAGYLA"
     gene            complement(7948..8688)
                     /gene="fnr"
                     /locus_tag="PKN25_10885"
     CDS             complement(7948..8688)
                     /gene="fnr"
                     /locus_tag="PKN25_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HNV91447.1"
                     /translation="MRASVPITVDGLKSACSQCNLVELCLPFGMSDRELSRLDELVGA
                     RRKVKRQHNLYRAGDSFEAIYAIRAGSFKTDVLLEDGREQVTGFQMTGEILGLDGIST
                     EAHSCNAVALEDSEVCVIAYDKLEQMSHEIEGLQLQFHKVMSREIVRDHGVMMLLGSM
                     RAEERLAAFLLNMSQRFTARGFSPSEFNLRMTREEIGSYLGLKLETVSRAFSRFQEDG
                     LIAVQQKHVHLLDLAGLKRLIQHQHGAR"
     gene            8829..10220
                     /gene="hemN"
                     /locus_tag="PKN25_10890"
     CDS             8829..10220
                     /gene="hemN"
                     /locus_tag="PKN25_10890"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNV91448.1"
                     /translation="MKSQTDLIFDPQLIRKFDINGPRYTSYPTADRFVEAFNAEALRA
                     WLAKRAVGGVSKPLSLYFHIPFCNTICYYCACNKIITKDHGRSAKYLKYLAKEIEMQA
                     ACLEGSRQVTQLHLGGGTPTFLSHDEMRELMAAVREHFTLVPNGEYSIEVDPRKVDFD
                     TVKLLAELGFNRMSVGVQDFAEDVQQAVNRIQSFDETKLVIDAARANGFKSVSMDLIY
                     GLPRQNPTSFDRTLDQVLEISPDRISLYSYAHLPGLFKPQRRINSGDMPTADTKLALL
                     QLGIRRLTEAGYVYIGMDHFAKPDDELTIAQRQGRLHRNFQGYSTHAECDLLAFGVSA
                     IGKIGPVYAQNVKTLDEYYDALDRDELPVLRGIELTADDLLRRAIIQALMCHFELSMQ
                     SIEIAYLVNFREYFAEELADLQEMEKAGLVKIDGDWISVQPGGRLLVRGIAMVFDRYL
                     RADRERQRYSRVI"
     gene            10292..11002
                     /locus_tag="PKN25_10895"
     CDS             10292..11002
                     /locus_tag="PKN25_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNV91449.1"
                     /translation="MPETGYLAVFLIGLLGGTHCVSMCGGIVGALSMQVHVPGSRPQW
                     PLHLAYNLGRIGTYTLLGACVGLLGSVGLLYEGMLPVQMTLYVLANLMLVALGLYLTG
                     FTRLLAPIERVGHLLWRRIQPATRRFLPARSVGQALPLGMLWGFIPCGLTYSILATAL
                     VTGSGARGAGLMLAFGLGTLPNLLLAGMLFKRFRDITRHPRVRLASGLLVLGFGLFGL
                     YHAPSLGGDLWRGVVCVT"
     gene            11049..11258
                     /locus_tag="PKN25_10900"
     CDS             11049..11258
                     /locus_tag="PKN25_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNV91450.1"
                     /translation="MNETTIKVEGMSCGGCVKNVTGVLKALPGVEDVRVSLEGASATV
                     RHDPAKVSIDALRAAVEGAGFDSPA"
     gene            11349..11876
                     /gene="ppa"
                     /locus_tag="PKN25_10905"
     CDS             11349..11876
                     /gene="ppa"
                     /locus_tag="PKN25_10905"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNV91451.1"
                     /translation="MGFEQVSAGKDVPNDINVIIEISAQGDPIKFEVDKDSGAVFVDR
                     FMGTSMRYPLNYGYVPHTIAGDGDPVDVLVVTPFPLQPGVVIRCRPVGVLKMEDDGGV
                     DAKVVAVPVSKLTPLYDKVQTTDDLPELLMKQTVHFFEHYKDLEPGKWVKVLGWGTVE
                     DAKAEIVNGLAAAKK"
BASE COUNT         1997 a         4012 c         3866 g         2116 t
ORIGIN      
        1 tcttcaccta catgtgggcg ggtccgcacc acctgcacta caccgcgctg cccgactgga
       61 cccagtccgt gggcatggtg ttctcgctga tcctgctggc gccgtcctgg ggcggcatga
      121 tcaacggcgt gctgaccctg tcgggcgcct ggcacaagct gcgcaccgac ccgatcctga
      181 agttcctcat cacctcgctg tccttctacg gcatgtcgac cttcgaaggc ccgatgatgt
      241 cgatcaagac ggtcaacgcg ctgtctcact acaccgactg gaccgtcggc cacgtgcact
      301 ccggcgcgct cggctgggtg gcgatgatct ccatcggctc ggtgtacttc ctgctgccgc
      361 gcctctacgg caagagcgag atgtacagcg tcaagctgat caacgcccac ttctggatcg
      421 ccaccatcgg catcgtgctg tacatcgcct cgatgtggat ctctggtgtg atgcagggcc
      481 tgatgtggcg cgcgaccaac ccggacggca cgctgaccta ctccttcgtc gagtcggtca
      541 aggccagcta cccgttctgg acgatccgct tcatcggcgg cgcgatgttc ctcggcggca
      601 tgctgatcat gttctacaac atggtgaaga cgatcgccgg ccagaaggcc tatgacgccc
      661 cggtgctcgc gcccgcggct gcccacgcct gatcggagaa caacaacatg gctcaatcga
      721 aacacgaaaa gatcgaacgc aacgtattcc tcatgatcgt gctcacgctg atgaccgtca
      781 gcgtgggtgg tctggtggag atcgtgccgc tgttcttcca gcactcgacc accaccgcga
      841 tcgaccacaa gggcaatgcg ctcgacgtca agccgtacga tccggtccgt ctcgtggggc
      901 gcgacatcta cgtccgcgaa ggctgctaca actgccactc gcagatgatc cgtccgttcc
      961 gcgccgagac cgagcgctac ggccactatt cggtcgccgg cgagtacatc tacgaccacc
     1021 cgttccagtg gggctccaaa cgcaccggtc cggacctcgc ccgcgtcggt ggccgctact
     1081 ccgacgaatg gcagcgtgtg cacctgctca atcctcgcga cgtcgtgccc gagtcgaaca
     1141 tgccagcctt cccctggctc gatcgcccgg tcagggcggc ggacatccag gaccgcatgc
     1201 gcgcgctcaa caaggtcggt ctgcacaagt acagcgacga ggagatcgcc gccgcgccgg
     1261 ccgcgctcga gggcaagacc gaactcgacg ccgtcgtggc ctacctccag gggttgggca
     1321 ccgcgatttc gcacttcagg taaggaagca ctcgcgccgt ggatatcaac gatttccgta
     1381 gcctgatcac cgtgcttggg ctgctctgct tcctgggtat ctgtgcctgg gcgtacagca
     1441 agcaggcgaa ggccggtttc gacgaggcgg cgcgtctgcc cctgagcgac gacgacctgc
     1501 cggccccgcg cggccggcaa gaccaagagg gaaaaacaaa tggctgactt catcagcggt
     1561 ttctggaaca tgtatgtgat ggtcctcgtg gccctctcca tcctgttctg tgtgttcgtg
     1621 ctggcgtcga acatgaccaa gcgcgagaaa gggccggtgc aattgcatgg ccatgtgtgg
     1681 gatgaaacgc ttgcggagta caacaacccc ctgccgcggt ggtggatgta cctgttctgg
     1741 atcaccatcg tgttcgcggt ggtttacctg gcgatctatc ccggctttgg ccgcagcaac
     1801 cagagccgcg gtcagtgggc gcagtacgat gcggagatgc aggccgccca ggagcgtttc
     1861 ggtcccgtgt tcgccaagta tcgcgagatg gacgtgatgg cggtcgccgc cgatcccgaa
     1921 gccaacgcga tgggcaagcg cctgttcctg acctactgcc agcagtgcca cggcgcggcg
     1981 gcgcagggtg cgaagagctt ccccaacctg accgacagcg agtggctgtg gggcggtgcg
     2041 ccggaaaaca tcaaggagac catcacgaac gggcgtaacg gcgtgatgcc tccgttcggt
     2101 cccgcgctcg gcgccgacgg tgtcaaggat gtcgccaact acgtgcgttc gatgaacggc
     2161 ctcgcccacg actccgtgcg cgcgcagcgc ggcaaggaag ccttcgagtc caactgcgtg
     2221 gcttgccacg gtgcggatgc caagggcaac ccgatgctcg gcgcgcccaa cttgaccgac
     2281 aaccgctggc tgtacggctc gtccgaggcg gtgatcatcg agaccgtcac caacggccgc
     2341 aacaacaaga tgcccgcctt cggtgccttc ctcggcgagg acaaggtgca cctgctcgcc
     2401 gcctacgtgc tgagcctgag caagaagtaa gcgtcacccg cagtagctgc agcagcatcg
     2461 catccccggg aggaaggggc cctcccgggg ttctcgataa caacaagaac aagaattcat
     2521 cggtaacaag gccatgaaaa gcgcatcccc cgagccgcag aaggcggcaa gaccctccaa
     2581 ccccgagtcc tcccaggaca cgctctacgc ggcacgcaag aagctctacg tgcgctccgt
     2641 caccggcctg ttcgccaact ggcggtgggc gttggtgtgg atcacccaga tcttgttcta
     2701 cggtctgccc tggctgagct ggaacgaccg ccaggccgtg cttttccatc tgaccgaacg
     2761 caagttctac atcttcggct gggtcttctg gccgcaggac gtgttctttc tcgcgatcct
     2821 gttgatcatc tcggcctacg ccctgttctt cttcaccgcg atcgcgggcc gcctgtggtg
     2881 cggatatgcc tgcccgcaga cggtgtatac cgagatcttc atgtggatcg aggagaagat
     2941 cgagggcgac catacccgcc gccagaagct cgaccgcgcg ccgatgagct ccggcaaggc
     3001 gttgcgccgc gccgtcaagt tcctcgtctg gggcatcgtc gcgctgtgga ccggcttcac
     3061 cttcgtggcc tatttctccc cgctcgacga attgctggcc tccttcaacg gcctcacctt
     3121 cgggccctgg gaaacgttct ggatcctgtt ctacgccggc ttcacctacc tcttcgccgg
     3181 cgtgctgcgc gagcaggtgt gcaagtacat gtgcccgtac gcgcgcttcc aggcggtgat
     3241 gttcgacccc gacaccctgg tgatcaccta cgacgaggca cgtggcgagc cgcgcggaac
     3301 gcgcaagaag ggcatcgatc cgcgctccgt gtccaagggc gactgcatcg actgcggcat
     3361 ctgcgtgcag gtttgcccga ccgggatcga catccgcgat ggcctgcagt acgaatgcat
     3421 cggctgtgcc gcctgcatcg acgcctgcga ccaggtcatg gacaagatga actacccgcg
     3481 cggtctgatc cgctactcga ccgagaacgc gatgaagcgc aattggggct ccaaggagat
     3541 cctcgcgcac gtgttccggc cgcgcacgct gatctacggc accatcctgg ccgcgatctg
     3601 cgctgccttc gtgtggggcc tggctgcgcg cgaaccgctg cgcgtcgacg tgctgcgcga
     3661 ccgctcctcg ctcggacagg agatcgaggg tggcctgatc gagaacgtgt accagctcca
     3721 ggtcatgaac atgaccgagt cgccgcgtgc gttcagcatc ggcgtctccg gcctgaacgg
     3781 aatccgcatc aagggtccgc agcgggtcga ggtcgcgccg gccggcgtgc agtcggtcac
     3841 cgttcaggtg cacgtcccct acgacgaagg caagccgggg gccaacacga ttttcttcga
     3901 cgtcgctgcg gaggatgatc ccgccttgac gctcaaggaa gagtccactt tcctggtacc
     3961 gcgctgacat gcgaagcaag aagcccgaag ccccggtcga gccctggtac cgccagggct
     4021 ggccctggtt cctcatcgcc ctgcccgcga ccgcggtggt cgccggcatc gccaccctgg
     4081 tgatcgccgc gaagaccttc gacggcatgg tggtcgacga ctactacaag gaaggccagg
     4141 cgatcgtgca gaccctcggc cgcgtcgagc acgcccgcga gctcggcttg cgcgccgcct
     4201 tgcgcatccg cagcgactcg atccacgtgg agctgacggc taccgcgccg ggcagcctgc
     4261 ccgacagcat ccgcctgacc gtcgcccacc ccacgcggag tggcctcgac caggagctgc
     4321 tcgtccgcgg gcgctccggt gtgtatgagg ccacgctcgc accgctgagc accggtcgct
     4381 ggttgttcgt gatacaagac gaggcccgaa gctggagaat ggacgggtcc gcttacctcc
     4441 ctgcagagat ggagatccgg atggatccga atgtctgagt ttcttgcaaa gggagaagca
     4501 ccatggcctg ggaattgttg ttcaagagcg atatcggtct gctgagcctc ttcaccatcg
     4561 tcttcattct ggtgatggcc gtctaccttt accgcttcgc ccgccgccat atggcggagg
     4621 acgagcagaa ggcccgtagc gcccgctcat gagcgatccg caccgcggat gaaaacggcc
     4681 cgcacgagcg ggccgcatgc tttggggggg ccgcgcgcgg ggccgatgcc ggtgtcagcg
     4741 gtagaccaga accggcgtct tgctgtgggt cagcaccttt tgcgtctcgg agccgagcag
     4801 caggcctgcg atgccgcgac ggccgtgcga ggccatgaag atgaggtcgc agccatggcg
     4861 gtcggcggcg tcgatgatcg cctcgtaggg cacctcattg accgcggtgt cgctgtcgca
     4921 ggccacgccc gccgcttccg ccgcaccgcg cgcctttgaa aggatcgcct cggcttcgga
     4981 tgcagccgat ttcgcgaact gctcgggcgt ggtcggatcg atcagcgcgc cctcgccgta
     5041 gatcggcatc gggaagtccg gctgcgaata gaagaaggtg atgcgcgcgc cggtctcctt
     5101 cgcgaaggag acggcacggg cgaccgtgct gtccgagagt gccgagccgt cggtcggtac
     5161 caggatgtgc ttgaacatga cgtacgctcc ctgtggtgtg tcgtgcttcg attatagcga
     5221 gcggatgggg tccgccatga ttgatcatga tcaagccgtc gagcgatcac gaagggcaca
     5281 gtgggaaccc ctgaaagatc ggcgaggcca gcgatgatgc tcaccttcct tggcgctgcg
     5341 ggcgaggtca ccggctcctg cctgcgggtc gatcacgagc gcggtgcatt tctggtggat
     5401 tgcggccttt tccagggcgg gcgcgaagcc gaccgcaaga accgcgacgc cctcgatttc
     5461 gagctgcgcg acatcgattt cgtgctcctc acccacgccc atctcgatca ctccggcctg
     5521 ctgccgcgcc tcgtggcact gggatatcga gggcgcatct tcgccaccac ggccactgtc
     5581 gatctgctcg gcgtgatgtt gatggattcc gcccatatcc aggagaagga ggccgagtgg
     5641 gcgctgcgcg acacgcgctc gcgcaagatg gcacgccggg cggaggtggc cccgctctac
     5701 accgtggagc aggcgcgtac cagtctgggc cgtttcgtcc ccaccgacta cgacgactgg
     5761 atcgagcccg gcccgggcgt gcgctgccgc ttccgtgacg ccgggcacat cctcggttcg
     5821 gcgatcctcg agatcgacgt cggcgagcgc ggccacaccc gccggctggt cgcctcgggc
     5881 gacattggcc agccgatgcg cccggtgctg cgggacccgg tgcggatcgc ccgggccgac
     5941 tacctgtgcg tcgagtcgac ctacggcaac cgcgcccacc gctccttcgc cgccacctgc
     6001 gacgagctcg cgctcgcctt gcggcgcacg ctcgaggtcg atggcggcaa cgtgatcatc
     6061 ccggcgttcg cggtcgggcg tacccaggaa gtgctgttcg tgctcgccga cctggtgcgc
     6121 gccggacgca tcgcccggct cgacgtcgtg gtcgactcgc cgatggcctc tgcggtcacc
     6181 gcgctcaccg tgcgccacac cgccctctgg gacgatgaga cgcgtgcgct ctacgcctgg
     6241 gccgaggcca actcgggacg cttccgtgtg cggttcgtgc aggacgtcga ggaatcgaag
     6301 gcgctcaatg cggtgccggg cgggctggtc gtgatctcgg caagcggcat gtgcgatgcc
     6361 ggtcggatca agcaccacct gcgccacaac ctcggccggc gcgagtgcgc agtggtcatc
     6421 gcaggcttcc aggctgccgg cacgctcggc cgccggctgg tcgacggtgc gcaccaggtc
     6481 accctgttcg gcgaacgtat cccggtgcgt gcgcgcatcc ataccatcgg cgggctctcc
     6541 gcccacgccg accagcccgc actcctcgac tggctgcgcg gcttcggcga ggcgccgcgg
     6601 cgcaccttcg tggcgcacgg cgagccggcc gcctccgccg ccttcgtcga ggcggtcgag
     6661 cgcgaactgg gctggagcag ggtcgaggcg gcgcggcccg gcgtgccggt cgtgctcgag
     6721 tgagcgtccg cgcgcaagcg gaccgggagg gcggcgaggg ccgctcaggc gttgtagcgc
     6781 gacagccgta ccggatcgtg cagactcaca cgcttgccct gcacggtgat cagaccggcg
     6841 tcgctgaggt cgtggaagat gcgcgacagg gtctcgggcg tcaggttgag gcgcgaggcg
     6901 atcacctgct tgctcgtcgg cagcgcgatc tcgcagggtg cgtcgttgtc ggccacctgc
     6961 agcaggtagc cgatcacgcg ctgggtgctc gagcgcaagg agtaggtctc cacgtcctgg
     7021 atcagcccgt gcaggcggat cgccatgccg gcgagcagct tgcgcgcgaa ggtggagtcc
     7081 tggtcgatga gttcgctcac caccgcctgg ccgacgtgca ccagcacggt ctcgctcagg
     7141 gcctcggcga agaccgggta ggggtggttc atgaacatca cggcctcgcc gaagctctgc
     7201 atcgggccga tgatctcgac caccttctcg gtgccttgcg aggacgagaa ggcgagcttg
     7261 acctggccgg agaccacgaa gtaaaagccg tgcggcaagt cgccgcgctg gaacagcact
     7321 tcgccgcggg cgacctggcg ctcgcgggtg tagcgcgcga ccagctggat gtccgcctcc
     7381 gacagttcgc tgaacagcgg gatgcggcgc agcagggagg ggatgtcgag cggggtcgtg
     7441 ttgggcatct ggaactctcc gtctggggac gcaatttcaa ttcggccggt tgtcgagcgg
     7501 cgccctgacc ggctttcgtt aggatcgtgg atgtactacc tgatcaagaa cctgcatgtg
     7561 acctgcgtcg tgctgagcgc cgccggcttc ctgctgcgcg gcacctggat gctcaccggc
     7621 aacgccctgc tgcagcaccg cctgacgcgg ctcctgccgc acctcgtcga cagcacgctg
     7681 ctcctgtctg cgatcacgct ggcggtaatg atcgggcaat accccttcag cgccggctgg
     7741 gtcacggcga aggtgatcgg cctgctcgcc tacatcctgc tcggcacgtt ggcgctcaag
     7801 cgcggacgta cccgtggcgt gcggctcgcc gccttcgccg gcgcgatcct ggtctatgcg
     7861 tggatcgtgt cggtggcgct gagcaagaac gtcgccggct acctcgcctg agccgtcgag
     7921 ccccgggtcg tcccggccac ggcgtgctca gcgcgcgccg tgctggtgct ggatcaggcg
     7981 cttgaggccg gcgaggtcga gcaggtgtac gtgcttctgc tgcaccgcga tcagcccgtc
     8041 ctcctggaag cgcgagaagg cgcgcgacac cgtctcgagc ttgagtccga ggtaggagcc
     8101 gatctcctcg cgcgtcatgc gcaggttgaa ctcgctcggc gagaaaccgc gcgccgtgaa
     8161 gcgctgcgac atgttcagca ggaaggcggc caggcgctct tcggcgcgca tcgagccgag
     8221 cagcatcatc acgccgtggt cgcgcacgat ctcgcggctc atgaccttgt gaaactgcag
     8281 ttgcaggcct tcgatctcgt gcgacatctg ctcgagcttg tcgtaggcga tcacgcacac
     8341 ctcgctgtcc tcgagcgcca ccgcgttgca gctgtgggcc tcggtgctga tgccgtcgag
     8401 accgaggatc tcgccggtca tctggaagcc ggtcacctgt tcgcgaccgt cctcgagcag
     8461 cacgtcggtc ttgaacgagc ccgcgcggat cgcgtagatc gcctcgaagg agtcaccggc
     8521 gcggtacaga ttgtgctggc gcttgacctt gcggcgcgcg cccaccagtt cgtcgagccg
     8581 gctcagctca cgatcggaca ttccgaaggg gaggcacaac tcgacgaggt tgcactgcga
     8641 acaggccgat ttgagcccat ccacggtaat tggcacgcta gccctcatct gcacgataat
     8701 cctgctttcc tgacattaga gacgcctgcg ctggagtggc gggattgcta atcacacccg
     8761 ttgacccaag tcaaccaaca ttacggaggc ttctgccatt gttcggcaga cagcccgcca
     8821 gcaagatcat gaaaagccag accgacctca tcttcgaccc gcaactgatc cgcaaattcg
     8881 acatcaacgg gccgcgctac acgtcctatc ccacggcgga ccgcttcgtc gaagccttca
     8941 atgcagaggc gctgcgggcc tggctggcca agcgggcggt gggcggcgta agcaaaccgc
     9001 tctcattata cttccacatc cccttctgca acacgatctg ctactactgc gcctgcaaca
     9061 agatcattac caaggatcat gggcgctcgg ccaagtacct gaaatatctc gcgaaagaaa
     9121 tcgagatgca ggccgcttgc ctggagggga gccgccaggt cacccagctc cacctcggcg
     9181 ggggcacgcc gacctttttg tcgcacgacg agatgcgcga gctcatggcg gcggtgcgcg
     9241 agcacttcac gctggtgccc aacggcgaat actcgatcga ggtcgacccg cgcaaggtcg
     9301 atttcgacac cgtgaagctg ctcgccgagc tcggcttcaa ccgcatgagc gtgggcgtgc
     9361 aggatttcgc cgaggacgtg cagcaggcgg tcaatcgtat ccagagcttc gacgagacca
     9421 agctggtcat cgacgccgcg cgcgccaacg gcttcaaatc ggtgagcatg gacctgatct
     9481 acggcctgcc ccggcagaac ccgaccagct tcgaccgcac gctcgaccag gtgctggaga
     9541 tctcgcccga ccgcatctcg ctgtacagct acgctcacct gccgggcctg ttcaagccgc
     9601 agcgccgcat caacagcggc gacatgccca cggccgacac caagctcgcg ctactgcagc
     9661 tcggcatccg ccggctcacc gaggcgggct acgtgtatat cggcatggac cacttcgcca
     9721 agcccgacga cgagctcacc atcgcccagc gccagggccg cctgcaccgc aacttccagg
     9781 gctattccac ccacgccgaa tgcgacctgc tcgccttcgg ggtgtcggcg atcggcaaga
     9841 tcggtccggt gtacgcgcag aacgtgaaga cgctcgacga gtactacgac gcgctcgacc
     9901 gcgacgagct gccggtgctg cgcggcatcg agctcaccgc cgacgacctg ctgcggcgcg
     9961 cgatcatcca ggcgctgatg tgccacttcg agctctcgat gcagtcgatc gagatcgcct
    10021 acctggtcaa cttccgcgag tacttcgccg aggaactcgc cgatctgcag gaaatggaga
    10081 aggccgggct ggtcaagatc gacggcgact ggatcagcgt gcagcccggc ggccgcctgc
    10141 tggtgcgcgg catcgcgatg gtgttcgacc gctacctgcg cgccgaccgc gagcgccagc
    10201 gctactcgcg ggtgatctga acgccgcacg gattgcgcac cgcagcgtag aatgagggcc
    10261 tcgcatttcc cgcggggccc ttttcttctt catgcccgaa accggttatc tcgccgtctt
    10321 cctgatcggc ctgctcggcg gcacccactg cgtgtcgatg tgcggcggca tcgtcggcgc
    10381 gctcagcatg caggtgcatg tgcccggcag ccggccgcag tggccgctgc acctggccta
    10441 caacctcggg cgcatcggca cctacacctt gctcggtgcc tgtgtcggcc tgctcggctc
    10501 ggtcggcctg ctctacgagg gcatgctgcc ggtgcagatg acgctgtacg tgctcgccaa
    10561 cctgatgctg gtggcgctcg ggctctacct caccggcttc acccgcctgc tcgcgcccat
    10621 cgagcgcgtc ggtcacctgc tgtggcggcg catccagccc gccacccggc gcttcctgcc
    10681 ggcgcgctcg gtcgggcagg cgctgccgct cggcatgctg tggggcttca tcccctgcgg
    10741 gctcacctat tccatcctcg ccactgcgct cgtcaccgga tcgggtgcgc gcggtgccgg
    10801 cctgatgctg gccttcgggc tgggcacgct gcccaacctg ctgctggcag gcatgctctt
    10861 caaacgcttc cgcgacatca cccgccaccc cagggtgcgc ctggcctccg ggctcctcgt
    10921 gctcggcttc ggcctgttcg ggctgtacca cgccccctcg ctcggtggcg acctgtggcg
    10981 cggcgtggtg tgcgtgacct gagggtgcga cggcacgccc ctttcaaccc atcgggagtc
    11041 ctggataaat gaacgaaacg acgatcaagg tcgaaggcat gagctgcggc ggctgcgtga
    11101 agaacgtcac cggcgtgctg aaggcgctgc ccggcgtgga ggacgtgcgg gtgtcgctgg
    11161 aaggcgcatc cgccaccgtg cgccacgatc cggccaaggt gtcgatcgac gcgttgcgcg
    11221 ccgcggtcga gggcgcgggc ttcgattccc cggcctgatc ggcagcctta gccgggggca
    11281 tcgtcgcagt gcaagacatt gtggtttccc cctataatcc gcgcacaatt tttccacggg
    11341 agggatgtat gggcttcgaa caggtcagcg ccggcaagga cgtgccgaac gacatcaacg
    11401 tcatcatcga gatctccgcc cagggcgatc cgatcaagtt cgaggtggac aaggacagcg
    11461 gcgccgtctt cgtcgaccgc ttcatgggca cctcgatgcg ctacccgctg aactacggct
    11521 acgtcccgca caccatcgcc ggcgatggcg acccggtcga cgtgctggtc gtgaccccgt
    11581 tcccgctgca gccgggcgtg gtcatccgct gccgtccggt cggcgtgctg aagatggaag
    11641 acgacggcgg tgtcgacgcc aaggtcgttg ccgtgccggt gtcgaaactg accccgctgt
    11701 acgacaaggt gcagaccacc gacgacctgc ccgagctgct gatgaagcag acggtgcatt
    11761 tcttcgagca ttacaaggac ctcgagcccg gcaaatgggt caaggtcctg ggctggggca
    11821 cggtcgagga tgccaaggcc gagatcgtga acggcctggc ggctgccaag aagtaatccc
    11881 gggaacgcac gtttccggat gacccggggc ccacgcatgc gtgggccccg gccgtttacg
    11941 gcgcggctga cgtgacactg acgggattgg cagtgccttg tcggccatgg g
//
LOCUS       DAOQBS010000095        11984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4455_length_11984_cov_15.640959, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000095 DAOQBS010000000
VERSION     DAOQBS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11984
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4455_length_11984_cov_15.640959"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..156
                     /gene="nadA"
                     /locus_tag="PKN25_10910"
     CDS             4..156
                     /gene="nadA"
                     /locus_tag="PKN25_10910"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNV91452.1"
                     /translation="MCSTMQRIDPQHLAWTLENLAEGKVVNQIKVPAHEAELARIALD
                     RMLAVS"
     gene            complement(242..673)
                     /locus_tag="PKN25_10915"
     CDS             complement(242..673)
                     /locus_tag="PKN25_10915"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNV91453.1"
                     /translation="MSALRPEVSIHTLNEAGGEYLPGYLGIEMLALDERRLNSRMPVK
                     KLHAAPNEFLHAASVVALADTTCGYATIAHLPAGADSFTTIELKSNHLGTVREGAIAC
                     SATAQHLGRTTQVWDATVTDEASGRVIALFRCTQMVLWPRG"
     gene            783..1712
                     /locus_tag="PKN25_10920"
     CDS             783..1712
                     /locus_tag="PKN25_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV91454.1"
                     /translation="MSWLPAWESFVRVVEGGSMAAAARTLGCTRAQVSKQVAELERRL
                     GAALFERSTRRLVLTPAGEVFLRHAARALEAVDSSEIAVRNLGERPSGTLRVSATLAF
                     GRHFVAPLLPVVTAANPGLEIELVLTDRLVDLVDDRIDLALRMTARPPEDAVARHLLR
                     LRWVLCAASAYVERHGSPREPAELAQHACIGHPAIGAAPWGLVAAAGAQARVAVRSRV
                     RFDSLDCTLDAVLAGHGIAILPAYLCEPALQAGSLRTVLDGWEPAFDVGRDLYACYTP
                     GRVGVSKVRVFMDALQQRLQPLPSWACAAGVGG"
     gene            1870..2619
                     /locus_tag="PKN25_10925"
     CDS             1870..2619
                     /locus_tag="PKN25_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNV91455.1"
                     /translation="MPLRICSYNIHKGFSQFNRRMVVHELRDRLRSLDADLVFLQEVQ
                     GLHLGHPARHPDWPALPQHEFLAEDAWQQIAYGGNAIYDHGHHGNAILSRHLILSTAN
                     QDVSDHRFERRGLLHCEVQLPEFDVPVHCVCVHLGLMAGSRRRQMEALAERMEELAPG
                     NAPLIIAGDFNDWRNRADDLLGRRLGLAEAFGSGRGRPARSYPSTLPFFRLDRIYVRG
                     FRVRQAQVHYGAPWARISDHAALTADLEPVG"
     gene            2619..3773
                     /gene="clsB"
                     /locus_tag="PKN25_10930"
     CDS             2619..3773
                     /gene="clsB"
                     /locus_tag="PKN25_10930"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HNV91456.1"
                     /translation="MQFLAGNAVALLENGEQYFPALEAEIDRAEREIFLQSYIFEDDG
                     TGARIAAALARAAGRGVAVRVMVDGFGGRSFVSRLMPRLREAGVQVLIYRRELRTLSL
                     RRHRLRRLHRKVVAIDGRVAFVGGINVVDDFDAGALPHPRYDYAVRVEGPVLAPVVAS
                     AQRLWRLVAWASFRRRAAQPLLAPPLTDAAGSIRAAFVVRDNLRHRHAIEEAYLDAIA
                     GAREEIVLANAYFFPGRRFRQALIDAARRGVRVTLLLQGVSDHPMQVYATRALYPIFL
                     DAGIRLFEYHRSHLHAKVAVIDHQWATVGSSNIDAFSLLLAREANVFVDDRGFAAELR
                     ASLETALCAGARELRRSDWRRLPLLRRGLSWLAYQLVRLAIGIAGYGGAH"
     gene            complement(3820..4242)
                     /locus_tag="PKN25_10935"
     CDS             complement(3820..4242)
                     /locus_tag="PKN25_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNV91457.1"
                     /translation="MHPNDISTQPEAIRGLLQQARTIAVVGISAKPDRPSHEVAHYLQ
                     RAGYTIVPVNPAYGEVLGQKCYPSLHEVPGKIDIVDVFRKPADVMAVVDEAIAVGAGS
                     VWLQLGVIAPAAADKAAAAGLKVVSDRCTKIEHRRLIG"
     gene            complement(4252..5208)
                     /gene="gluQRS"
                     /locus_tag="PKN25_10940"
     CDS             complement(4252..5208)
                     /gene="gluQRS"
                     /locus_tag="PKN25_10940"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNV91458.1"
                     /translation="MQQPLPSPCPGTGHPADPCVGRYVGRFAPSPSGALHFGSLVAAV
                     GSILDARAHGGRWLLRIEDVDTPRTVPGAAEGILATLERFGFEWDGAVVRQSERGDAY
                     AAALARLCAAGLAYPCACTRRELADQPLARDGSRRYPGTCRGGLPPGRAGRAWRVRAE
                     GVVRFCDAVQGEQEIDLAADAGDYVVKRADGLFAYQLAVVVDDAEAGVTHVVRGADLL
                     DSTARQIHLLGLLGQPVPAYAHLPLATNAAGEKLSKQTLARAIDAHPPQVALMAALRF
                     LGHNPPDMLATAPLAETWAWARAHWSLAAVPRRRQAVADGFS"
     assembly_gap    5254..5353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5452..6366)
                     /locus_tag="PKN25_10945"
     CDS             complement(5452..6366)
                     /locus_tag="PKN25_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNV91459.1"
                     /translation="MQAPSRPAAPGTRPIVVCADDYGLAPGVSEAIAALVETGRLSAT
                     SCMSALPDWRRAAPPLRALMTRAPADVGLHLTLTDQPPLSAAARRAWGERLPPLGRLL
                     AAALARRLPADAVADEIRAQFDAFEDAWGDAPDHVDGHQHVHVLPGVREALIAELLRR
                     HPRGRVWVRDCVEPPLRCLRRREAVGKAWFISALGRGLHRRLREAGLPCNAGFSGLHD
                     FSTERPFAPKMRRFLAATGPRPLVHVHPGRVDAALRACDPLTTPREAELAYLASTEYL
                     ADLAAAGLHPARFAAFDALDGGTTTPPP"
     gene            6550..7560
                     /locus_tag="PKN25_10950"
     CDS             6550..7560
                     /locus_tag="PKN25_10950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV91460.1"
                     /translation="MSHSDSLDAHPHVPPASSAHPRLSVVVPLYNEGETVEALHRRLS
                     QVLAALELSDWEIVCVDDGSRDDTYARVAALCAGDPHLKAIRFARNFGKEAAMAAGLQ
                     AAGGDVIVLMDGDLQHPPELIPEMLARWRGGAMMVTAVRRSRVTDPWLRRKLTRGFYA
                     FFSKVSEVELAEGGGDFRVFDRAVVDAINSLPERTRFMKGITSWVGFRQEVVEFEPAQ
                     RAGGVSGWSMLRLLRYAIDGLSAFSTLPLRVWSVIGLMMAGLSGLYGLFLVLRTMLFG
                     IDLPGYVSLMVSGLFMSGIQLISLGVLGEYVGRIFTEVKGRPLFLVSERLGFERRPER
                     DR"
     gene            7557..9197
                     /locus_tag="PKN25_10955"
     CDS             7557..9197
                     /locus_tag="PKN25_10955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNV91461.1"
                     /translation="MIPPGGTAGAPAAAASVGAHGAIARGLALAVLALAGVFVLLTFD
                     QHGISNDEEVQHVYGRLLLDFYASGFADRQAFEYKNLYLYGGFFDLLAAAFERAGVAE
                     GPALWDLRHLISAVFGLLGLAGTWLLARRLAGEWAGLAALVLLSITGSWSGAMFTHTK
                     DIPFATTMLWALYFSVRVLDTLPAPPWRVLAGLGVALGCAFGLRIGAVFAVFYLGVGV
                     LAATALQPGGRVRFLLRGVLALLPAAAIALALGALFWPWAAMEPGNVLTAMRAFSHFS
                     FELDTVLAGRVMNVGEVPGHYLAAYLLVRLPELFLAGLALALLLGVRAVPALAGEQAL
                     RAALPWLPVVLAALFPLVYTLLAAPPLYNGLRHFSFVLPPLAVLAGMGLVRAWHGLSV
                     RPPLLRRAVLGACALAVLGQLGQLARLHPYEYLAYNRLAGGVQGAVGRWEQDYWASSL
                     REAVHALNALVAREGGAGRHYSVAVCAEPLQAQVWLAPGLRATRDWWGADFYLSPTHM
                     GCDEAMRGRVVAQVERAGLVLAVVKDRRALVGEERRPR"
     gene            9194..9604
                     /locus_tag="PKN25_10960"
     CDS             9194..9604
                     /locus_tag="PKN25_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNV91462.1"
                     /translation="MSPSGGCARFLRFAAVGAVGTLAHYALLLALVEGADAPPLAGAI
                     AGFMLGALVNYALARRLVFASTRSHAQALPRFFAVALAGLAWTALLMSFFMELLGLHY
                     LLAQVLTTALLLLWHYAGNALWTFRRGRRDTESR"
     gene            9601..10506
                     /locus_tag="PKN25_10965"
     CDS             9601..10506
                     /locus_tag="PKN25_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV91463.1"
                     /translation="MSAQAPAGTPPHRLEADLLLVLTTLIAAAGWIFSKEALTGMPPL
                     IFIGLRFAIAGLVLALFSLPQLRALDRRGLRNSLLVGALFAGGMVLWILGLSHSSHLG
                     EASFISSMGIVMVPVFARLFFGDRPPASTWFALPLALAGFACLSLDGGFEVELGQWFF
                     LGAALVFALMFNVNSHIVRNVPVRALSVVQMLAVGVLVLPPALLIETWPQAWSAPVLG
                     WLLASALLATTLRFLLQLHGQSLTTPSHAALIMMLEPVWTALAAAWWFAETMSTLQLA
                     GCGLIFLALVVSRWAWIRGLFGGRG"
     gene            10528..11052
                     /gene="def"
                     /locus_tag="PKN25_10970"
     CDS             10528..11052
                     /gene="def"
                     /locus_tag="PKN25_10970"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNV91464.1"
                     /translation="MAVRTLLRMGDARLLQPAAPVGEFGTPALAALIEDLFDTMAAHG
                     GVGLAAPQIGVGLQVVIFGFERSERYPDAAPVPRTILLNPVIVPLTEEREEGWEGCLS
                     VPGLRGMVPRATRIRYTGYTPAGEPIERFAEGFHARVVQHECDHLAGVLYPMRVRDFR
                     RFGFTDVLFPELRG"
     gene            11142..>11984
                     /locus_tag="PKN25_10975"
     CDS             11142..>11984
                     /locus_tag="PKN25_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV91465.1"
                     /translation="MRLKTCLGCGVVVLASLWLSLAHAADKAVLFENVFVFDGRSSER
                     GRSPVNVLVVGDTIQTISAAPIAPPADAELTRVAGGGRTLMPGLIDAHWHSMLIGQKL
                     VDAMTSDVGYTNLRAAQVAEATLMRGFTTVRDMGGPVQGLRRAIEEGRFPGPRIFPSG
                     AMISQTGGHGDFRLPHEVPRAASQGLSHTELTRTAAIADGADEVLRRTREQLMLGATQ
                     IKLMAGGGVTSIYDPIDATQYTLDEIRAAVAAAENWGTYVTVHAYTSRAVQVAIEAGV
                     KAVEH"
BASE COUNT         1443 a         4129 c         4411 g         1901 t
ORIGIN      
        1 tgcatgtgct cgaccatgca gcgcatcgac ccgcaacacc tggcgtggac gctggagaac
       61 ctcgccgagg gcaaggtggt gaatcagatc aaggtgcccg cgcacgaggc cgagctcgcg
      121 cgcatcgcgc tcgaccgcat gctcgcggtg tcctgaacac gcgatccgcg ctcgcaagaa
      181 ccgggggcgt ctcccgcgag ggggggcgcc cccgttgcgt ccaccgggcg gtgaagccgc
      241 ctcagccgcg cggccagagg accatctgcg tgcagcggaa gagcgcgatc acccgcccgc
      301 tggcctcgtc ggtgacggtc gcgtcccaca cctgggtggt gcgtccgagg tgctgggcgg
      361 tcgcgctgca tgcgatcgcg ccctcgcgca ccgtccccag gtggttgctc ttgagctcga
      421 tggtggtgaa ggagtccgcg cccgcgggca ggtgggcgat ggtcgcgtag ccgcaggtgg
      481 tgtcggccag cgcgacgacg cttgcggcgt ggaggaactc gttcggtgcc gcgtgcagct
      541 tctttaccgg catccggctg ttcagccggc gctcgtcgag cgccagcatc tcgatgccga
      601 ggtagccggg caggtactcg ccgccggcct cgttcagggt gtggatgctg acttcgggac
      661 gcagtgcgct catctggggc tccttcatga agattcgcaa tggaaagcgt tgatgaggat
      721 agtcttctta ttgcttccgg ataagccgat aatccggttc gttgcgtcaa cggatcattg
      781 acatgagctg gttaccggcc tgggagagct tcgttcgcgt ggtcgagggc ggcagcatgg
      841 cggccgcggc gcgcacgctc ggctgcacgc gtgcccaggt cagcaagcag gtcgccgagc
      901 tcgaacgccg cctcggcgcc gccctcttcg agcgctccac ccggcggctc gtcctcaccc
      961 ccgccgggga ggtgttcctg cgccacgcgg cgcgcgcact ggaggccgtg gacagcagcg
     1021 agatcgcggt gcgcaacctc ggcgagcgcc cgagcggcac gctgcgggtg agcgcgacgc
     1081 tcgccttcgg gcgtcacttc gtcgcgcccc tgctgcccgt cgtcacggcg gcgaaccccg
     1141 ggctggagat cgagctggtg ctcaccgacc gcctggtcga cctggtggac gaccgcatcg
     1201 acctcgcgct gcgcatgacc gcgcggccgc cggaggatgc ggtcgcgcgc cacctgctgc
     1261 gcctgcgctg ggtgctctgc gcggcgagcg cctatgtcga gcgccacggc agcccgcgcg
     1321 agccggccga gctggcgcag catgcgtgca tcggccaccc cgcgatcggc gccgccccct
     1381 ggggattggt cgccgcggct ggcgcgcagg cccgcgttgc ggtgcgcagc cgcgtccgtt
     1441 tcgacagcct cgattgcacg ctcgacgcgg tcctcgccgg ccacggcatc gccatcctgc
     1501 ccgcctacct gtgcgagccg gcgctgcagg ccggcagcct gcgcaccgtg ctcgacggct
     1561 gggagcccgc gttcgacgtc ggccgcgacc tgtacgcctg ctacacgccg ggcagggtgg
     1621 gggtatccaa ggtgcgcgtc ttcatggatg cgttgcagca gcggctgcag ccgctgccgt
     1681 cgtgggcctg cgccgcgggt gtgggcggat gagaaaggcg gtgaaagccc cggcgaaagg
     1741 tggcgagccg gggcgggggc acggccgacg aatccgtgcc gtcgatcgtg cgccggaggc
     1801 tttaacatcc gcttcccgcc cgcatatgat gtcgcgtggc cttcaccccg gccggagtcg
     1861 cctccgctca tgcccctgcg tatctgctcc tacaacatcc acaagggttt ctcgcagttc
     1921 aaccgccgca tggtggtgca tgaactgcgc gaccgcctgc gcagcctgga tgccgacctc
     1981 gtctttctgc aggaggtgca gggcctgcac ctgggccatc cggcacgcca ccccgactgg
     2041 cccgcgctgc cgcagcacga gttcctcgcc gaggacgcct ggcagcagat cgcctatggc
     2101 ggcaacgcga tctacgacca tggccaccac ggcaacgcga tcctgagtcg ccacctcatc
     2161 ctcagcaccg ccaaccagga cgtctccgac catcgcttcg agcgccgcgg cctgcttcac
     2221 tgcgaggtgc agctgcccga gttcgacgtg ccggtgcatt gcgtgtgcgt gcatctgggc
     2281 ctgatggcgg gcagccggcg gcgccagatg gaggcgctcg ccgagcgcat ggaagagctc
     2341 gccccgggca acgcgccgct gatcatcgcc ggcgacttca acgactggcg caaccgcgcc
     2401 gacgacctgc tcgggcgccg gctcgggctt gccgaggcct tcggcagcgg acgcgggcga
     2461 ccggcgcgca gctacccgag cacgctgccc ttcttccgcc tcgaccgcat ctacgtgcgc
     2521 ggcttccgcg tgcgccaggc gcaggtgcat tacggcgcgc cgtgggcgcg catctccgac
     2581 catgcggcac tgaccgccga cctggagccg gtgggctgat gcagttcctc gcgggcaacg
     2641 cggtcgcgtt gctggagaac ggcgagcagt acttcccggc gctggaagcc gagatcgacc
     2701 gtgccgagcg cgagatcttc ctgcagagct acatcttcga ggacgacggt accggcgcgc
     2761 gcatcgccgc tgcactcgca cgcgcggcgg gccgcggggt cgcggtgcgg gtgatggtgg
     2821 acggcttcgg cgggcgcagc ttcgtctcgc ggctgatgcc gcgcctgcgc gaggccggcg
     2881 tgcaggtgct gatctaccgc cgcgagctgc gtaccctgtc gctgcgccgc catcgcctgc
     2941 gccggctgca ccgcaaggtg gtggcgatcg acggccgcgt ggccttcgtc ggtggcatca
     3001 acgtggtgga cgacttcgat gcgggcgcgc tgccgcaccc gcgctacgac tacgcggtgc
     3061 gcgtcgaggg gccggtgctc gcgccggtgg tggcctcggc gcagcgcctg tggcggctgg
     3121 tggcgtgggc gagctttcgc aggcgcgccg cccagccgct gctggcgccg ccgctgaccg
     3181 acgcggcggg ctcgatccgc gccgctttcg tcgtgcgcga caacctgcgc caccgccatg
     3241 cgatcgagga ggcctatctc gatgcgattg ccggcgcgcg cgaggagatc gtgctcgcca
     3301 atgcgtactt cttcccgggg cggcgcttcc gccaggcgct gatcgacgct gcgcggcgcg
     3361 gcgtgcgggt gaccctgctg ctgcagggcg tgtccgatca cccgatgcag gtgtatgcga
     3421 cgcgcgcgct gtatccgatc ttcctcgacg ccggcatccg cctgttcgaa taccaccgca
     3481 gccatctgca cgccaaggtg gcggtcatcg accaccagtg ggccaccgtc ggttcgagca
     3541 acatcgacgc cttcagcctg ctgcttgcgc gcgaggccaa cgtcttcgtg gacgatcgcg
     3601 gtttcgcggc ggaactgcgc gcgagcctgg agacggcgct gtgcgccggc gcgcgcgaac
     3661 tccggcgttc ggactggcgg cgcctgccct tgctgcgccg cggcctgagc tggctcgcct
     3721 accagctcgt gcgcctggcg atcggcatcg ccggctacgg cggcgcgcac tgagctcgcg
     3781 acgacgctgg cgcgaatgcc gggcgcggcg tgcgcggctt cagccgatca ggcggcggtg
     3841 ctcgatcttg gtgcagcggt cgctcaccac cttcaggccg gcggcggcgg ccttgtccgc
     3901 ggctgcgggc gcgatcaccc cgagctgcag ccacacgctg ccggcgccca cggcgatggc
     3961 ctcgtccacc accgccatca cgtctgccgg cttgcggaac acgtcgacga tgtcgatctt
     4021 gcccggcacc tcgtgcaggc tgggatagca cttctgacca agcacctccc cgtaggccgg
     4081 gttgaccggg acgatggtgt aaccggcgcg ctgcaggtag tgcgcgacct cgtgactggg
     4141 gcgatccggc ttggccgaga tgccgaccac ggcgatcgtg cgcgcctgct gcagcaggcc
     4201 gcggatggcc tcgggttgcg tgctgatgtc gttcggatgc atggggtttc ctcaggagaa
     4261 gccgtcggcc accgcctggc ggcggcgcgg cacggcagcg agcgaccagt gggcccgcgc
     4321 ccacgcccac gtttcggcga gcggcgcggt ggcgagcatg tcgggtggat tgtggccgag
     4381 aaagcgcagc gcggccatca gggcgacctg cggcggatgc gcgtcgatcg cgcgcgccag
     4441 ggtctgcttg gagagcttct cgcccgcggc gttggtggcc agcggcaggt gggcgtaggc
     4501 gggcaccggc tggccgagga ggccgagcag gtggatctga cgcgcggtcg aatcgagcag
     4561 gtcggcgccg cgcacgacgt gggtcacgcc cgcctcggcg tcgtccacca ccacggcgag
     4621 ctgataggcg aagagcccgt cggcgcgctt gaccacgtag tcgccggcgt cggcggcgag
     4681 gtcgatctcc tgctcgccct gtaccgcgtc gcaaaaacgc accacccctt ccgcacgcac
     4741 gcgccaggcg cggcccgcgc gccccggcgg caggccgccg cggcaggtgc ccgggtagcg
     4801 gcgcgagccg tcgcgcgcga gcggctggtc ggcgagttcg cgtcgggtgc aggcgcaggg
     4861 ataggcgagg ccggcggcgc acaggcgcgc gagcgcggcc gcgtaggcgt cgccgcgttc
     4921 gctctggcgc acgaccgcgc cgtcccattc gaagccgaag cgttccagcg tggcgaggat
     4981 gccctcggca gcgccgggga ccgtgcgcgg ggtatcgaca tcctcgatcc gcagcagcca
     5041 gcgtccgccg tgggcgcgcg catccaggat gctgccgacc gcggcgacca gcgagccgaa
     5101 gtgcagcgcg ccgctgggcg agggggcgaa gcggccgacg tagcgaccga cgcaggggtc
     5161 ggcggggtgg cctgtaccgg ggcaggggga agggaggggc tgctgcatgg cgaggatcgt
     5221 atcgaccggg cgggcgcttg tgcaacgcgc ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnccgacat tcgaggccgt cgacccgttc ggcggcgtct cgcgccaagg
     5401 gtggctgcgg cgcggtatcc ggagccgaag ccccggctgc ggtggatcgc gtcagggggg
     5461 tggagtggtc gtcccgccat cgagtgcatc gaacgccgcg aagcgcgcag gatgcaggcc
     5521 ggcggcggcg aggtcggcga ggtactcggt ggaggcgagg taggcgagct cggcctcgcg
     5581 cggggtggtg agcggatcgc aggcgcgcag cgcggcgtcc acccggccgg ggtgtacgtg
     5641 caccagcggg cgcgggccgg tggcggcgag gaagcggcgc atcttcggcg cgaacgggcg
     5701 ctccgtggaa aaatcatgca ggccgctgaa gcccgcgttg cagggcaggc cggcctcgcg
     5761 caggcggcgg tggagcccgc gcccgagggc ggagatgaac caggccttgc ccaccgcctc
     5821 gcggcgtcgc aggcagcgca gcggcggctc gacgcagtcg cgcacccaca cgcggccgcg
     5881 cgggtggcgg cgcagcagct cggcgatcag cgcttcgcgc acgccaggca gcacatgcac
     5941 gtgctggtgg ccgtccacat ggtcgggtgc gtcgccccag gcgtcctcga aggcgtcgaa
     6001 ctgggcgcgg atctcgtccg ccacggcgtc cgccggcagg cggcgggcga gggcggcggc
     6061 gagcaggcgg ccgagcggcg gcaggcgctc gccccaggcc cgccgtgccg cggcgctgag
     6121 cggaggctgg tcggtcaggg tcaggtgcag gccgacgtcg gccggcgcgc gtgtcatcag
     6181 tgcgcgcaac ggcggcgcgg cgcggcgcca gtcgggcagc gcgctcatgc agctcgtggc
     6241 cgacagccgg ccggtctcga cgagcgcggc gatggcttcg ctgacaccgg gggcgaggcc
     6301 gtagtcgtcg gcgcacacca cgatcggacg ggtgcctgga gcggcagggc gggagggggc
     6361 ttgcatgtga tcggcgctgc gggcggaaag cccgcagtat cccgcacccg gtcctcccgc
     6421 ggtgcagtcc gtgcggcgtt gcccgtccct gcgagccagc cgtgctccgg gcgcgccggg
     6481 cagggcgcga atggtttatc gtggacgcct tcgctccggc tgcggagccc actcgccggt
     6541 tgttccatcg tgtcgcactc cgactcgctc gatgcccacc cccatgtgcc gcccgcctcc
     6601 tcggcacacc cccgactctc ggtcgtggtc ccgctctaca acgagggcga aacggtggag
     6661 gcgctgcatc ggcgcctgtc gcaggtgctt gcggcgctgg agctgtccga ctgggagatc
     6721 gtctgcgtcg atgacggcag ccgcgacgac acctatgccc gcgtggccgc cctgtgcgcg
     6781 ggtgaccccc acctcaaggc gatccgcttc gcgcgcaact tcggcaagga ggcggcgatg
     6841 gccgccgggc tgcaggcggc tgggggcgac gtgatcgtgc tgatggacgg cgacctgcag
     6901 catccgcccg agctgatccc cgagatgctc gcgcgctggc gtggcggggc gatgatggtc
     6961 accgcagtgc ggcgctcgcg cgtcaccgat ccctggctgc ggcgcaagct gactcgcggc
     7021 ttctacgcct tcttcagcaa ggtctccgag gtcgagctcg ccgagggcgg gggcgacttc
     7081 cgcgtcttcg atcgcgcggt ggtcgacgcg atcaacagcc tgcccgagcg cacccgcttc
     7141 atgaagggga tcaccagctg ggtgggcttc cgccaggagg tggtcgagtt cgagcccgcg
     7201 cagcgcgccg gcggcgtctc cgggtggtcg atgctgcgcc tgctgcgcta tgcgatcgac
     7261 ggcctgtccg ccttcagcac cctgccgctg cgggtctggt cggtgatcgg cctgatgatg
     7321 gcggggctct cgggcttgta cggacttttc ctcgtgctgc gcacgatgct gttcggcatc
     7381 gacctgcccg gctacgtgtc gctgatggtg tcggggctgt tcatgtccgg catccagctc
     7441 atcagcctgg gggtgctcgg cgagtacgtg gggcgcatct tcaccgaggt caagggccgg
     7501 ccgctgttcc tggtgtcgga acggctcggc ttcgaacgca ggccggagcg cgatcgatga
     7561 tcccgccagg cgggacggcg ggtgcgccgg ccgcggcggc atccgtcggg gcgcatggcg
     7621 cgatcgcgcg cgggctcgcc ctcgccgtgc tggcgctggc cggggtgttc gtgctgctca
     7681 ccttcgacca gcacggcatc agcaacgacg aggaggtcca gcatgtctac ggccgcctgc
     7741 tgctcgactt ctacgcctcc ggcttcgccg accgccaggc cttcgaatac aagaacctct
     7801 atctttacgg gggcttcttc gacctcctcg cggcggcctt cgagcgcgcc ggggtggccg
     7861 aagggccggc gctgtgggac ctgcgccacc tgatctcggc cgtcttcggt ctcctcgggc
     7921 tggccggcac ctggctgctc gcgcgccggc tcgcgggcga gtgggcgggg ctggcggcgc
     7981 tcgtgctgtt gtcgatcacc ggctcgtggt cgggcgcgat gttcacccac accaaggaca
     8041 tccccttcgc caccacgatg ctgtgggcgc tgtacttcag cgtgcgcgtg ctcgacacgc
     8101 ttcccgcgcc gccgtggcgc gtgctcgcgg ggctgggtgt ggcgctcggc tgcgccttcg
     8161 gcctgcgcat cggtgcggtg ttcgcggtgt tctaccttgg cgtcggcgtg ctcgcggcga
     8221 cggccttgca gccgggcggg cgggtgcgtt tcctgcttcg cggcgtgctg gcgctgctgc
     8281 cggcggcggc gatcgcgctg gcgctgggcg cgctgttctg gccgtgggcg gcgatggagc
     8341 cgggtaacgt gctcacggcg atgcgcgcgt tctcgcattt cagcttcgag ctcgacaccg
     8401 tgctggccgg gcgcgtgatg aacgtcggcg aggtcccggg gcattacctc gcggcctacc
     8461 tgctggtgcg cctgccggag cttttcctcg ccgggctcgc gctcgcgctg ctgctcggcg
     8521 tgcgcgccgt gccggcgctc gctggcgagc aggccctgcg cgcggccctg ccgtggctgc
     8581 cggtggtgct ggcagcgctg ttcccgctcg tctacaccct gcttgcggcg ccgccgctgt
     8641 acaacgggct gcgccatttc agcttcgtgc tgccgccact ggcggtgctg gcgggcatgg
     8701 ggctcgtgcg cgcgtggcac ggcctgagcg tccgtccgcc gctgctgcgc cgcgccgtgc
     8761 tgggcgcgtg cgccctggcg gtcctcggcc aactcggcca gctcgcccgc ctgcatccct
     8821 acgagtacct cgcctacaac cgcctcgcgg gcggcgtgca gggggcggtc gggcgctggg
     8881 agcaggacta ctgggcgagc agcctgcgcg aggcggtgca cgccctcaac gccctggtcg
     8941 cgcgcgaggg gggggcaggg cgacactatt ccgtggccgt gtgcgccgag ccgctgcaag
     9001 cccaggtgtg gctcgcgccc gggttgcgcg cgacgcgcga ctggtggggg gcggacttct
     9061 acctctcccc cacccacatg ggttgcgacg aggccatgcg gggccgcgtg gtggcgcagg
     9121 tcgagcgtgc ggggctggtg ctggcggtgg tcaaggatcg gcgcgcgctg gttggcgagg
     9181 agcggcggcc ccgatgagcc cgtccggcgg atgcgcgcgt ttcctgcgct ttgccgcggt
     9241 gggggcggtg ggtacgctcg cccactacgc gctgttgctc gcgctggtgg agggcgcgga
     9301 tgcgccgccg ctggcggggg ccatcgccgg attcatgctt ggcgcgctgg tcaactacgc
     9361 cctcgcgcgc cggctggtgt tcgcgtccac gcgcagccac gcccaggccc tgccgcgctt
     9421 cttcgccgtg gcgctggccg ggctggcgtg gacggcgctg ctgatgagtt tcttcatgga
     9481 gctgctcggc ctgcactacc tgctcgccca ggtgctgacg accgcgctgc tgctgctgtg
     9541 gcactacgcc ggcaacgccc tgtggacctt ccgccgcggc cggcgcgaca cggagagccg
     9601 atgagcgcac aggctcccgc cggtacgccg ccgcaccgcc tcgaggccga cctcctgctc
     9661 gtgctcacca cgttgatcgc cgcggcgggc tggatcttct ccaaggaggc gctcaccggc
     9721 atgccgccgc tgatcttcat cggcctgcgc ttcgccatcg ccgggctggt gctggcactg
     9781 ttctcgctgc cgcagctgcg cgcgctcgat cggcgcggac tgcgcaacag cctgctcgtc
     9841 ggcgcgctgt tcgccggcgg catggtgctg tggatcctcg gcctgtcgca cagcagccac
     9901 ctcggcgaag cctcgttcat ctccagcatg ggcatcgtca tggtgccggt gttcgcgcgc
     9961 ctgttcttcg gcgatcgtcc gccggcgagc acctggttcg cgctgccgct cgcactcgcc
    10021 ggcttcgcct gcctgtcgct cgacggcggc ttcgaggtcg agctcgggca gtggttcttc
    10081 ctcggcgcgg cgctggtgtt cgcgctcatg ttcaacgtca actcgcacat cgtacgcaac
    10141 gtgccggtgc gcgcgctcag cgtggtgcag atgctcgccg tcggcgtgct ggtgctgccg
    10201 ccggccttgc tgatcgagac ctggccgcag gcctggagcg cgccggtgct cggctggctg
    10261 ctcgcgagcg cgctgctcgc caccacgctg cgcttcctgc tccagctcca cggccagagc
    10321 ctgaccacgc ccagccacgc ggcgctgatc atgatgctcg agccggtgtg gaccgcgctc
    10381 gccgcggcgt ggtggttcgc cgagaccatg agcacgctgc aactcgccgg ctgcgggctg
    10441 atcttccttg ccctcgtggt gagccgctgg gcgtggatcc gcggcctctt cggcgggcgc
    10501 ggctgaacga tcggggagag agattccatg gctgtccgta cccttctgcg catgggtgat
    10561 gcgcgccttc tgcagccggc cgcgccggtc ggcgaattcg gcacgcctgc gctcgccgcg
    10621 ctcatcgagg acctgttcga caccatggcc gcgcacggcg gtgtgggcct ggccgcgccg
    10681 cagatcggcg tcggcctgca ggtggtgatc ttcggcttcg agcgcagcga gcgctacccg
    10741 gacgccgcgc cggtgccgcg caccatcctg ctgaacccgg tgatcgttcc gctcacggaa
    10801 gagcgcgagg agggctggga gggctgcctg tcggtgcccg gcctgcgcgg catggtgccg
    10861 cgcgccacgc gcatccgcta caccggctac acgcccgcgg gcgagccgat cgagcgcttc
    10921 gccgagggct tccatgcgcg cgtggtgcag cacgagtgcg accacctcgc cggcgtgctg
    10981 tatccgatgc gggtgcggga tttccgccgc ttcgggttta cggacgtgct gtttccggag
    11041 ctgcggggat agccaaggcc tggctttatc cccaggacgg gggtggcggt ccggactgct
    11101 atcctgatga ttgtttcgat cacgtccaga ggggtcgtcc gatgaggctg aaaacctgtc
    11161 tcggttgcgg ggtcgtcgtg ctcgcatcgc tgtggctgtc gctggcgcat gcggcggaca
    11221 aggccgtcct gttcgagaat gtcttcgtct tcgatggcag gagcagcgag cgaggccgct
    11281 cgccggtcaa cgtgctggtg gtgggcgaca ccatccagac catctcggcg gcgccgatcg
    11341 cgccgcccgc ggacgccgag ctgacgcgtg tcgccggcgg ggggcgcacg ctgatgcccg
    11401 gcctgatcga tgcgcactgg cactcgatgc tgatcggcca gaagctggtc gatgccatga
    11461 ccagcgacgt cggctatacc aatctgcgtg cggcgcaggt cgccgaggcg acgctcatgc
    11521 gcggcttcac cacggtgcgc gacatgggcg ggccggtgca gggcctgcgg cgtgcgatcg
    11581 aggaggggcg tttccccggg ccgcgcatct tcccgtcggg cgcgatgatc tcgcagaccg
    11641 gcggccatgg cgatttccgt ctgccgcacg aggtgccgcg cgccgccagc cagggtctca
    11701 gtcataccga acttacccgc accgccgcca ttgccgatgg cgccgacgag gtgctgcggc
    11761 gcacccgcga acagctgatg ctcggcgcga cgcagatcaa gctgatggct ggcggtgggg
    11821 tgacgtcgat ctacgacccg atcgacgcca cccaatacac cctggacgag atccgcgccg
    11881 cagtggctgc ggcagagaac tggggcactt acgttacggt gcacgcctac accagccgtg
    11941 cggttcaggt cgccatcgag gccggggtca aggcggtgga gcac
//
LOCUS       DAOQBS010000096        11972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4467_length_11972_cov_14.527650, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000096 DAOQBS010000000
VERSION     DAOQBS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4467_length_11972_cov_14.527650"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PKN25_10980"
     CDS             complement(<1..749)
                     /locus_tag="PKN25_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV91466.1"
                     /translation="MAATSKPALSGFARALIQHGHLKEADAIACTTHAGEAATAFILE
                     VANRGLMSCAAMARFAAETFGYPLLDLAAFDAAMFAREAVDRKLLAKHQVAPLAKRQS
                     RITLATADPSNLRALDEIRFQTGLQIELVIVEADKLKRAVEALSESAADTLKELTGEA
                     FDMDLLQQDGPAQPRDEEEANEVDDAPVVKFIQKVLIDAINEGASDVHFEPYEKFYRI
                     RVRTDGILRDIAQPPLVLKDKIAARIKVISRL"
     gene            complement(838..2118)
                     /locus_tag="PKN25_10985"
     CDS             complement(838..2118)
                     /locus_tag="PKN25_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HNV91467.1"
                     /translation="MTDLTLTLQIVALVVLLTLSACFSMSETVMMAANRYRLRSLANQ
                     GSRGATLALDLLARTDRLLGVILLFNNLVNTGAATLVSVITLELFGNESWILGVATLL
                     ATFAILVFSEITPKVVGANHADRLAPFVAYPLTGLLRASYFAVWFINLFSRGLLNLMR
                     LQPKEETSSALSVEELRAMVIESGQYIPHKHRSMLLNLFDLESITVEDVMTPRGAIES
                     IDLSDPEDIVRRQIATSFHTRLAVYDGDSEHVIGVLHQRKLLGNTLEENFSVDRIRDL
                     LARPYFVPADTPLYSQIQFFQENQQRLGFVVDEYGEILGLITLEDIIEELIGKFTTST
                     PDVGDRMHWGEDGSVLVDGTSNLRELNRRLELALPIDGPKTLNGLILEHLQDIPETGV
                     SMKIADTPIEVVQTQDRMVRTARIFRPRIEGAGQ"
     gene            complement(2202..3032)
                     /gene="ccsA"
                     /locus_tag="PKN25_10990"
     CDS             complement(2202..3032)
                     /gene="ccsA"
                     /locus_tag="PKN25_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNV91468.1"
                     /translation="MRTILLHLLPASLYAGLGVFFWRTCMTRGSALEPRRECMSTRER
                     LLLLGAIVAHGAALHLAIFPGSEMRFGFGVAVSLMVWLAVCFYWVETLYTRLDGLHAA
                     VMPAGAIGSLVPMLFPGDHVLANATSPAFRAHFVVAMLAYSLFTLAALHAMLMSVAGR
                     QLHNARFSRLLSGLPPLLTMEALLFRLISIAFVLLTLTLASGVLFSESLFGQAFRVDH
                     KTVFAFVSWFLFGGLLVGRRLWGWRGRLALRWTLAGFVALMLAYVGSRFVIEVVLHRT
                     "
     gene            3081..4451
                     /gene="ffh"
                     /locus_tag="PKN25_10995"
     CDS             3081..4451
                     /gene="ffh"
                     /locus_tag="PKN25_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNV91469.1"
                     /translation="MLDNLTQRLSKVVKTLRGQARLTEENIQEAMREVRMALLEADVA
                     LPVVKDFVAKVREKAVGQEVIGSLTPGQALVGVVHDELKALMGGAHEGLDLSTQPPAV
                     VLMAGLQGAGKTTTTGKLAKLLHEQHKKKVLVVSTDVYRPAAIEQLKTVAAQAGVGFF
                     PSETAQKPVDIALAALDYARKHHMDVLLVDTAGRLAVDEAMMAEIQALHAAVKPIETL
                     FVVDAMLGQDAVNTARAFNEALPLTGIVLTKLDGDSRGGAALSVRHVTGKPLKFAGVG
                     EKLSGLEPFHPDRMASRILGMGDILGLVEDARRGVDEEKAKAFAQKLKTGKGFDLDDF
                     KEQIAQMRKMGGLSSMMDKLPAQFAQAAGKLQGGAEEKAISRIEGIINSMTPLERAKP
                     EILKASRKRRIAAGAGVTVQEVNRLLNQFEQTQKVMKQFSKGGMSKMMRAMKGMMPGG
                     LPGMPR"
     gene            4466..5242
                     /locus_tag="PKN25_11000"
     CDS             4466..5242
                     /locus_tag="PKN25_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNV91470.1"
                     /translation="MKDILVAFGRAGRSLGRRDMFWHLLWPGLLAVVIWAGVAFYAWT
                     PVTEWLYAAVSGWSFVGGWLSASETTAAIVLVLIQIATALLVVPLVYVTAAMLVATVA
                     LPLMLERVARTDYADLEQRRGGSNLGSAMNSIVAGVLFLLALVLSLPLWLIPGAGLLI
                     SVTLTGWLNQRAFGYDALMYHADKGELQRLRDAWRPQMLLLGGGTALLAYVPVINLVA
                     PAFAGLAFVHYMLETLRRHRIQHGITVLDAEPGADLRKLR"
     gene            5239..5988
                     /locus_tag="PKN25_11005"
     CDS             5239..5988
                     /locus_tag="PKN25_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNV91471.1"
                     /translation="MNFGALIIGDEILSGRRSDKHLAKLIELLGARGLKLSWARYVGD
                     DFARLAGTLRETFATGDVVFSFGGIGATPDDRTREAAGAALGLELALHPEAEAEIRAR
                     FGAEITPERLQMGVYPVGSEIIPNSFNRIPGFSIRQHYFTPGFPVMAWPMVEWVLDTK
                     YAHLHHAEDYVEQAVTVWDAYEGQLIGLMRQVTADFPDTTLFSLPTIAAEGQRRSLEL
                     GMKGTAARVAAAMAVIKADLSARGLEWEDKA"
     gene            5985..6752
                     /locus_tag="PKN25_11010"
     CDS             5985..6752
                     /locus_tag="PKN25_11010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNV91472.1"
                     /translation="MSARAAASEEPHSVLVEGTRVDLVLRRSARRSFALQVDHRGARV
                     SVPLRTPLGEVERFVHSHGAWLLDRLRARAQAPQALPLEVAEGACLPLFGRVLVLRRV
                     SARAARWRGLADGREELLLPAGVDPQRALVRALRARALAWYRGRVEEYCHRLGVAVPP
                     VSLSSAATRWGSCSTRSGIRLHWRLIHLEPALIDYVVAHEVAHLAEMNHSPRFWAVVE
                     RLYPDWRAARAALRSAAATLPVIATAAADRGAFTRED"
     gene            6780..7166
                     /gene="gloA"
                     /locus_tag="PKN25_11015"
     CDS             6780..7166
                     /gene="gloA"
                     /locus_tag="PKN25_11015"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HNV91473.1"
                     /translation="MRILHTMLRVGDLERSLAFYTEVLGMRLLRRQDYPDGKFTLAFV
                     GYQDEAHGAVLELTHNWGVDKYELGTAYGHIALEVADAKKACDDIRARGGKVVREAGP
                     MKHGTTVIAFVEDPDGYKVELIERKG"
     gene            complement(7199..8290)
                     /locus_tag="PKN25_11020"
     CDS             complement(7199..8290)
                     /locus_tag="PKN25_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV91474.1"
                     /translation="MKFPLDRPMQVAVVDDTQLNLFLIGKLVDRMQDARAHNFERPTA
                     ALEWCLANAPDLIIVDYMMPEMDGLEFIRRIRACRDRDDLPILMVTASHERDVRYAAL
                     DCGASDFLTKPVDAREFEARVRNMLKLRQAHLANEHRAETLAAEVRRATADILDRERE
                     TITRLSRAAEFRDPETGAHIQRMSHYSALIARRLGLGDTFAETLLMAAPMHDVGKLGI
                     PDTILLKPGRLTADEFAVMKRHPQIGHDILKGSSSSILRLGATIALTHHEKFDGSGYP
                     QGLSGQSIPMEGRIVAVADVFDALTSARPYKPAWPMSRAIALLREGRGTHFDPTCVDA
                     LFHTWEDVLAVQSRYQDEHAPEPPVSPPC"
     gene            complement(8368..9612)
                     /locus_tag="PKN25_11025"
     CDS             complement(8368..9612)
                     /locus_tag="PKN25_11025"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNV91475.1"
                     /translation="MKRVDDFRLKLGSQELVPIMVGGMGVDISTSDLALEAARLGGVG
                     HISDAMLPTVSDRRYNTKYVKNKLQQYKFNVANADKSVVQFDLGLIAEATATHVGRTM
                     ERKRGPGMVFINCMEKLTMNAPKETLRVRLRTAMDHGIDGITLAAGLHLGSLSLIEDH
                     PRFRDVQLGIIVSSLRALQLFLKKSARTGRLPDYVVVEGPLAGGHLGFGMDWAQYDLA
                     TIVAEIRDWMREQQLDIPLIPAGGIFTGTDAVNFLEAGAAAVQVATRFTVTKECGLPD
                     EVKQDYFKAEEDEIEVNQISPTGYPMRMLKSSPAIGSGIRPNCEAYGYLLDSSGNCQY
                     IEAYNREVAAHPEAKKVKVFDKTCLCTHMRNFDCWTCGHYTYRLKDTTVREDGGDYRI
                     LSAEHVFKDYQFSTEGRIALPD"
     gene            9753..10736
                     /locus_tag="PKN25_11030"
     CDS             9753..10736
                     /locus_tag="PKN25_11030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HNV91476.1"
                     /translation="MFKAVLIEKDDAGYRAAVKELDEAALPEGDVGVRVEYSSLNYKD
                     GLAITGRGAVVRKFPMVAGIDLVGTVESSAHPGVAVGDKVILNGWGTSEVHWGGLAQR
                     ARVRGEWLVPLPAAFTPRQAMAIGTAGYTAMLCVMALERHGVVPQRGEVLVTGASGGV
                     GSVAIALLSRLGFTVVASTGRPQEADYLRELGATTIIDRSELGLPGRALGKERWAGVV
                     DTVGSHTLANACATTLYRGTVAACGLAQGLDFPASVAPFILRGVTLVGIDCVMAPREE
                     RIEAWSRLARDLDVHKLERMTREIGLGEVVDTAAALLDGQERGRIVVDVDR"
     gene            10773..11351
                     /locus_tag="PKN25_11035"
     CDS             10773..11351
                     /locus_tag="PKN25_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302438.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HNV91477.1"
                     /translation="MTETISRFKVPELADLAEDVRERILAVQEKAGFVPNVFLMLAHR
                     PAEFRAFFDYHDALMEKDVGLSKAEREMIVVATSAAGQCLYCVIAHGAILRIRAKNPR
                     LADQLATNHRKAEISPRQRAMLDFAIKLSTRGAEVDEADLAVLRAHGFDDEAIWDIGA
                     ITAFFSMSNRLVHLTGTPPNEPFYLMGRVPKA"
     gene            11446..11773
                     /gene="ssrA"
                     /locus_tag="PKN25_11040"
     tmRNA           11446..11773
                     /gene="ssrA"
                     /locus_tag="PKN25_11040"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01850"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="betaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01850"
BASE COUNT         1883 a         3876 c         4310 g         1903 t
ORIGIN      
        1 aggcgcgaga tcaccttgat gcgcgcggcg atcttgtcct tgagcaccag cggcggctgg
       61 gcgatgtcgc gcaggatgcc gtcggtgcgc acgcggatgc ggtagaactt ctcgtagggc
      121 tcgaagtgga cgtccgaggc gccctcgttg atcgcgtcga tcagcacctt ctggatgaac
      181 ttgacgaccg gggcgtcatc gacctcgttg gcttcctcct cgtcgcgcgg ctgcgcggga
      241 ccgtcctgct gcagcaggtc catgtcgaag gcttcgccgg tgagctcctt gagcgtgtct
      301 gcggccgact cggacagggc ctcgaccgcg cgcttgagct tgtccgcctc gacgatcacc
      361 agctcgatct gcaggccggt ctggaagcgg atctcgtcca gcgcgcgcag gttggaaggg
      421 tcggcggtgg cgagcgtgat gcggctctgg cgcttggcga gcggcgcgac ctggtgcttg
      481 gcgagcagct tgcggtcgac cgcctcgcgc gcgaacatcg ccgcatcgaa ggcggcgagg
      541 tcgaggagcg gatagccgaa ggtctcggcg gcgaagcggg ccatcgccgc gcagctcatc
      601 aggccgcggt tggcgacctc gaggatgaag gcggtcgcgg cctcgcccgc atgggtggtg
      661 catgcgatcg cgtccgcctc cttgaggtgg ccatgctgga tcagggccct ggcgaagccg
      721 ctcagggccg gtttggaggt cgccgccatg ccttgctcgt gtccggactg cggccgatgt
      781 tgcccgccgc ccccggcctt gtaaagatgc gcgcagccgg tgccggggcg cgcgggctca
      841 ctgtccggcg ccctcgatcc gcggacggaa gatgcgcgcg gtgcgcacca tgcggtcctg
      901 ggtctgcacg acctcgatcg gggtatcggc gatcttcatc gacaccccgg tctccgggat
      961 gtcctgcagg tgttcgagga tcagaccgtt gagcgtcttc ggaccatcga tcgggagtgc
     1021 gagctcgagc cggcggttga gctcgcgcag gttggacgtg ccatcgacca gcacgctgcc
     1081 gtcttcgccc cagtgcatgc ggtcgcccac gtccggggtg ctggtggtga acttgccgat
     1141 gagttcctcg atgatgtcct cgagggtgat caggccgagg atctcgccgt attcgtccac
     1201 cacgaagccg agacgctgct ggttctcctg gaagaactgg atctgcgagt agagcggcgt
     1261 gtcggccggc acgaaatagg ggcgggccag gaggtcgcgg atgcggtcca cgctgaaatt
     1321 ttcctccagc gtgttgccga gcagcttgcg ctggtgcagc acgccgatca cgtgctcgct
     1381 gtcgccgtcg tagaccgcca gccgggtgtg gaagctggtg gcgatctggc ggcggacgat
     1441 gtcctcgggg tcggacaggt cgatgctctc gatcgctccg cgcggcgtca tcacgtcctc
     1501 gaccgtgatg ctctcgaggt cgaagaggtt gagcagcatg ctgcggtgct tgtgcgggat
     1561 gtactggccc gactcgatca ccatcgcgcg caactcttcg acggagagcg ccgagctcgt
     1621 ctcctccttc ggctgcagac gcatcaggtt cagcagcccg cgcgagaaca ggttgatgaa
     1681 ccataccgcg aaatacgacg cgcgcagcag gccggtgagt gggtaggcca cgaagggggc
     1741 gagacgatcg gcgtggttgg cgccgacgac cttgggcgtg atctcggaga acaccaggat
     1801 cgcgaaggtg gcgagcaggg tcgccacgcc gaggatccag ctctcgttgc cgaagagctc
     1861 gagcgtgatc acgctgacca gggtggcggc gcccgtgttg accaggttgt tgaacagcag
     1921 gatgacgccg agcagccggt ccgtgcgggc gagcaggtcg agggccaggg tggcgccccg
     1981 gctgccctga ttggcgaggg agcgcaggcg gtagcggttg gccgccatca tcaccgtctc
     2041 cgacatcgag aagcatgccg acagggtcag cagcacgacg agggcgacga tctgaagcgt
     2101 cagggtgagg tccgtcacga ggggcggtgg aaaggaaagg ggccccgagt attggagccc
     2161 ctcgtgacgg tgtcaatgca cgcggacttc ggcgggcgga ctcaggtgcg atgcagcacg
     2221 acttcgatga cgaagcggct gcccacgtag gcgagcatca gcgcgacgaa gccggccagg
     2281 gtccagcgca gcgccagccg gccgcgccag ccccacaggc ggcggcccac cagcaggccg
     2341 ccgaagagga accaggagac gaaggcgaac accgtcttgt ggtcgacccg gaaggcctgg
     2401 ccgaacaggc tctcggagaa cagcacgccg ctcgccagcg tcagcgtgag cagcacgaag
     2461 gcgatggaaa tcagccggaa cagcaacgcc tccatggtga gcaggggcgg caggccggag
     2521 agcaggcggc tgaagcgtgc gttgtggagc tggcgcccgg ccaccgacat cagcatcgcg
     2581 tgcagggcgg ccagggtgaa caggctgtag gcgagcatgg ccacgacgaa gtgggcgcgg
     2641 aaggccggcg aggtggcgtt ggcgagcacg tggtcgcccg ggaacagcat cggcaccagg
     2701 ctgccgatcg cgcctgcggg catcaccgcc gcgtgcaggc cgtcgagccg ggtgtagagg
     2761 gtctcgaccc agtagaagca caccgccagc cagaccatca gcgagaccgc gacgccgaag
     2821 ccgaagcgca tctcgctgcc ggggaagatc gccaggtgca gcgccgcgcc gtgcgcgacg
     2881 atggcgccca gcagcagcag gcgctcgcgc gtgctcatgc actcccggcg cggctccagc
     2941 gcggaaccgc gcgtcatgca ggtgcgccag aagaacacgc ccagaccggc gtaaagcgag
     3001 gcggggagga gatgaagtag aattgtgcgc atacgtagcc gcagtttact ccaagtcacg
     3061 cgcccaaggc ctgccccaac atgctcgaca atctgaccca acgcctgtcc aaggtcgtca
     3121 agaccctccg cggccaggcc cgcctgacgg aagaaaacat ccaggaggcg atgcgcgagg
     3181 tgcgcatggc cctgctcgag gccgacgtcg cgctgccggt ggtcaaggac ttcgtcgcca
     3241 aggtcaggga gaaggcggtc gggcaggagg tcatcggctc gctgacgccc ggccaggccc
     3301 tggtcggcgt ggtgcacgac gagctcaagg cgctgatggg cggcgcccac gagggtctgg
     3361 acctgtccac ccagccgccc gcggtggtgc tgatggccgg cctgcagggc gcgggcaaga
     3421 ccaccaccac cggcaagctc gccaagctgc tgcacgagca gcacaagaag aaggtgctgg
     3481 tggtctccac cgacgtctat cgcccggcgg cgatcgagca gctgaagacg gtggcagcgc
     3541 aggccggggt gggcttcttc ccctccgaga ccgcgcagaa gccggtcgac atcgcgctcg
     3601 ccgcgctcga ctacgcgcgc aagcaccaca tggacgtgct gctggtcgac accgccggcc
     3661 ggctcgcggt ggacgaggcc atgatggccg agatccaggc cctgcacgcc gcagtcaagc
     3721 cgatcgagac cttgttcgtg gtcgacgcga tgctcggtca ggatgcggtg aacaccgcgc
     3781 gcgccttcaa cgaggcgctg ccgctcaccg gcatcgtgct gaccaagctg gacggcgact
     3841 cgcgcggcgg cgcggcgctg tcggtgcgcc acgtcaccgg caagccgctc aagttcgccg
     3901 gcgtgggcga gaaactctcc gggctggagc ccttccaccc cgaccgcatg gccagccgca
     3961 tcctcggcat gggcgacatc ctcggcctcg tcgaggatgc gcgccgcggc gtggacgagg
     4021 agaaggccaa ggccttcgcg cagaagctca agaccggcaa gggcttcgat ctcgacgact
     4081 tcaaggagca gatcgcccag atgcgcaaga tgggcgggct gtcctcgatg atggacaagc
     4141 tgccggcgca gttcgcccag gcggcgggca agctgcaggg cggggccgag gaaaaggcga
     4201 tctcgcgcat cgagggcatc atcaactcga tgaccccgct cgagcgcgcc aagcccgaga
     4261 tcctcaaggc cagccgcaag cgccgcatcg ccgcgggcgc cggggtgacg gtgcaggagg
     4321 tcaatcgtct gctcaaccag ttcgagcaga cgcagaaggt catgaagcag ttctccaagg
     4381 ggggcatgag caagatgatg cgagcgatga agggcatgat gccgggcggc ctgccgggca
     4441 tgccgcgctg agggggggcg gacgcatgaa ggacatcctc gtcgccttcg ggcgcgccgg
     4501 tcgcagcctc gggcggcgcg acatgttctg gcacctgctg tggcccggcc tgctcgcggt
     4561 ggtgatctgg gccggcgtgg ccttctacgc gtggacgccg gtcaccgagt ggctctatgc
     4621 cgcggtgagc gggtggtcct tcgtcggcgg atggctgtcg gcctcggaga ccaccgcggc
     4681 gatcgtgctg gtgctgatcc agatcgccac cgcgctgctg gtggtcccgc tggtgtacgt
     4741 caccgccgcg atgctggtgg cgacggtggc cctgccgctg atgctcgagc gcgtcgcgcg
     4801 caccgactac gccgacctcg agcagcgtcg cggcggctcc aacctgggca gcgcgatgaa
     4861 ctccatcgtc gccggcgtgc tgttcctgct cgcgctggtg ctgtcgttgc cgctgtggct
     4921 gattccgggc gccggcctgc tgatctcggt caccctcacc ggctggctga accagcgcgc
     4981 cttcggctac gacgcgctga tgtaccacgc cgacaagggc gaactgcagc gcctgcgcga
     5041 cgcttggcgg ccgcagatgc tcctcctcgg cggcggcacg gccctgctcg cgtatgtgcc
     5101 ggtgatcaac ctggtggcgc ccgccttcgc cggcctcgcc ttcgtccatt acatgctcga
     5161 aaccctgcgc cggcatcgca tccagcacgg catcaccgtg ctcgacgcag agccgggtgc
     5221 cgacctgcgg aagctgagat gaatttcggt gcactgatca tcggcgacga gatcctttcc
     5281 ggccgtcgca gcgacaaaca cctcgccaag ctgatcgaac tgctcggtgc gcgcgggctc
     5341 aagctgtcgt gggcgcgcta cgtcggcgac gacttcgcgc gccttgccgg gacgctgcgc
     5401 gagaccttcg ccaccggtga cgtggtgttc agcttcggtg gcatcggcgc cacccccgac
     5461 gaccgtacgc gcgaggccgc gggcgccgcg ctcggactgg aactcgccct gcaccccgag
     5521 gcggaggccg agatccgtgc ccgcttcggt gccgagatca cccccgagcg cctgcagatg
     5581 ggcgtgtatc cggtgggcag cgagatcatc cccaacagtt tcaaccgcat ccccggcttc
     5641 tcgatccgcc agcactactt caccccgggc tttccggtga tggcctggcc gatggtggag
     5701 tgggtgctcg acaccaagta cgcccacctg caccacgccg aagattacgt cgagcaggcg
     5761 gtgacggtgt gggatgccta cgagggccag ctgatcggcc tgatgcgtca ggtcaccgcc
     5821 gacttccccg acaccaccct gttcagcctg cccacgatcg ccgccgaggg ccagcggcgt
     5881 tcgctcgagc tcggcatgaa aggcacggcg gcgcgcgtcg cggcggcgat ggcggtgatc
     5941 aaggccgatc tctccgcacg cgggctggag tgggaagaca aggcttgagc gctcgggcgg
     6001 cggcctcgga agagccgcac agcgtgctcg tcgagggcac ccgtgtcgat ctggtgctgc
     6061 ggcgctcggc gcggcgctcg ttcgcgctcc aggtggacca ccgcggcgcg cgcgtgtcgg
     6121 tgccactgcg caccccgctc ggcgaggtcg agcgtttcgt gcatagccac ggtgcctggc
     6181 tgctcgaccg tctgcgggcg cgggcgcagg cgccgcaggc gctgccgctg gaggtggcgg
     6241 agggcgcgtg cctgccgctc ttcgggcgtg tcctggtgct gcgccgggtg tcggcgcgcg
     6301 cggcgcgctg gcggggcctg gcggatggac gggaggaact cctgctgccc gcgggcgtcg
     6361 atccgcagcg ggcgctggtg cgcgcgctgc gcgcgcgcgc actggcctgg taccgcgggc
     6421 gggtggagga gtactgccac cgtctcggcg ttgcggttcc gccggtgagc ttgtcgagcg
     6481 cggccacgcg ctggggtagc tgcagcaccc gctcggggat ccggctgcac tggcggctga
     6541 tccatctcga gccggcgctg atcgactacg tggtcgccca cgaggtcgcg cacctggccg
     6601 agatgaatca ctcgccgcgc ttctgggcgg tcgtcgagcg tctttacccg gactggcgtg
     6661 ccgcgcgcgc cgcgctgcgc agcgcggcgg cgacgctgcc ggtgatcgcg accgccgccg
     6721 ccgaccgcgg cgccttcacc cgcgaagatt gaaaccacca cgatacagga agacacccca
     6781 tgcgaatcct tcacaccatg ctgcgcgtcg gcgatctcga gcgctcgctc gccttctaca
     6841 ccgaggtgct gggcatgcgc ctgctgcgcc gccaggacta tccggacggc aagttcaccc
     6901 tcgccttcgt cggctaccag gacgaggctc acggagcggt gctggagctg acccacaact
     6961 ggggcgtgga caagtacgag ctcggcaccg catacggcca catcgcccta gaggtcgcgg
     7021 acgcgaagaa ggcctgcgac gacatccgcg cgcgcggcgg caaggtagtg cgcgaggcgg
     7081 gaccgatgaa gcacggcacc accgtgatcg cctttgtcga ggatcctgac ggctacaagg
     7141 tcgaactgat cgagcgcaag ggctgacgca ggcgcccccg ggggtggggg cgcaggcctc
     7201 agcagggggg cgagacgggc ggctccgggg cgtgctcgtc ctggtagcgc gactgcacgg
     7261 cgagcacgtc ctcccaggtg tggaagagcg cgtcgacgca ggtcgggtcg aagtgggtgc
     7321 cgcgcccctc gcgcagcagc gcgatcgccc gcgacatcgg ccatgccggc ttgtagggcc
     7381 gtgcggaggt gagcgcatcg aacacgtcgg cgaccgcgac gatgcggccc tccatcggga
     7441 tcgactggcc ggagagtccc tgcgggtagc cgctgccatc gaacttctcg tggtgggtga
     7501 gggcgatggt tgcgcccagg cgcaggatgc tggacgacga gcccttgagg atgtcgtggc
     7561 cgatctgcgg gtggcgcttc atcaccgcga actcgtccgc agtcagtcgg ccgggcttga
     7621 gcaggatggt atcggggatg ccgagcttgc ccacgtcgtg catcggtgcg gccatcagca
     7681 gggtctcggc gaaggtgtct cccaggccca ggcggcgggc gatcagggcc gaatagtgcg
     7741 acatgcgctg gatgtgggcg ccggtctcgg gatcgcggaa ctcggccgca cgcgacagcc
     7801 gggtgatggt ctcgcgctcg cggtcgagga tgtcggcggt tgcccggcgc acctcggcgg
     7861 cgagggtctc ggcgcggtgc tcgttggcga ggtgggcctg gcgcagcttg agcatgttgc
     7921 gcacgcgcgc ttcgaattcg cgcgcatcga ccggcttggt gaggaagtcg ctcgcgccgc
     7981 agtcgagtgc cgcgtagcgc acgtcgcgct cgtggctggc ggtgaccatc aggatgggca
     8041 ggtcgtcgcg gtcgcggcag gcgcggatgc ggcggatgaa ttccagcccg tccatctcgg
     8101 gcatcatgta gtcgacgatg atgaggtcgg gcgcattggc gaggcaccac tcgagcgcgg
     8161 cggtggggcg ctcgaagttg tgtgcacgcg catcttgcat gcggtcgacg agcttgccga
     8221 tcaggaacag gttgagctgc gtgtcgtcga ccactgcgac ctgcatcgga cggtcgagtg
     8281 ggaatttcat ccgatgagta tcggaggcct tgggcctcgc accgaggaac tattgcgcgg
     8341 acggcatcgc atccgcgcat cctcggttca gtcgggcagg gcgatgcggc cttcggtgct
     8401 gaactggtag tccttgaaca cgtgctcggc ggacaggatg cggtagtcgc cgccgtcctc
     8461 gcgcacggtg gtgtccttga gccggtaggt gtagtggccg caggtccagc agtcgaagtt
     8521 gcgcatgtgc gtgcacaggc aggtcttgtc gaagaccttg accttcttcg cctcggggtg
     8581 cgcggcaacc tcgcggttat aggcctcgat gtactggcag ttgccgctgg agtcgagcag
     8641 gtagccgtag gcctcgcagt tggggcggat gccgctgccg atcgccgggc tgctcttcag
     8701 catgcgcatc gggtagccgg tgggcgagat ctggttgacc tcgatctcgt cctcctcggc
     8761 cttgaaatag tcctgcttga cctcgtcggg caggccgcac tccttggtga ccgtgaagcg
     8821 ggtcgcgacc tggacggccg cggcgcccgc ctcgaggaaa ttcaccgcgt cggtgccggt
     8881 gaagatgccg ccggccggga tcagcgggat gtcgagctgt tgctcgcgca tccagtcgcg
     8941 gatctcggcg acgatggtgg cgaggtcgta ctgcgcccag tccatgccga agccgaggtg
     9001 gccgccggcg agcgggccct cgaccaccac gtagtcgggg agccgcccgg tgcgcgcgct
     9061 cttcttgagg aagagctgca gcgcgcgcag cgagctgacg atgatgccca gctgcacgtc
     9121 gcggaagcgc ggatggtcct cgatgaggga gagcgagccc aggtgcaggc cggctgccag
     9181 tgtgatgccg tcgatgccgt ggtccatcgc ggtgcgcagg cgcacacgca gggtttcctt
     9241 gggcgcgttc atcgtcagct tttccatgca gttgatgaac accatgcccg gcccgcgctt
     9301 gcgctccatg gtgcggccga cgtgggtcgc ggtcgcttcg gcgatgaggc cgaggtcgaa
     9361 ctgcaccacc gacttgtcgg cgttggcgac gttgaacttg tactgctgca gcttgttctt
     9421 gacgtacttg gtgttgtagc gccggtcgga cacggtcggc agcatcgcgt cggagatgtg
     9481 tccgaccccg cccagccggg cggcctcgag cgccagatcg gaggtggaaa tatccacccc
     9541 catcccgccc accatgatcg gcacgagttc ctgggagccc agtttcaggc ggaagtcatc
     9601 tacacgcttc attgcttgga gtccgttgat tcggcagtcc gacattatca cccgagagcc
     9661 cctttgggcc caagttcgag cgccatgcgg gcatcgttta acatgcgggt tcgactgcga
     9721 aaccggcgcg aggtgcgccc agggagagaa aaatgttcaa ggcagtgctg atcgagaagg
     9781 atgatgccgg ctaccgcgcg gcggtgaagg agctcgacga ggccgcgctg cccgaaggcg
     9841 acgtcggcgt tcgggtggaa tactcttcgc tgaactacaa ggatggcctg gcgatcaccg
     9901 ggcgtggtgc ggtggtgcgc aaattcccga tggtcgcggg catcgacctc gtcggcacgg
     9961 tcgagtccag cgcgcatcca ggcgtggcgg tgggcgacaa ggtgatcctc aacggctggg
    10021 ggacgagcga ggtgcactgg ggcgggctcg cgcagcgtgc gcgcgtgcgc ggcgagtggc
    10081 tggtgccgct gcccgcggcc ttcaccccgc gccaggcgat ggcgatcggg accgccggct
    10141 acaccgcgat gctgtgcgtg atggcgctcg agcgccacgg cgtcgttccg cagcgcggcg
    10201 aggtgctggt gaccggcgcg agtggcggcg tgggcagcgt ggcgatcgcg ctgctctcca
    10261 ggctgggctt caccgtggtg gcctccaccg ggcgtccgca ggaggccgac tacctgcgcg
    10321 aactgggcgc gaccaccatc atcgaccgca gcgaactcgg cctccccggc cgcgcgctcg
    10381 gcaaggagcg ctgggcgggg gtggtcgaca cggtgggcag ccacaccctg gccaacgcct
    10441 gcgcgaccac cctgtaccgc ggcacggtcg ccgcctgcgg cctggcgcag ggactcgatt
    10501 tcccggcctc ggtcgcaccc ttcatcctgc gcggcgtcac tctcgtcggc atcgactgcg
    10561 tgatggcgcc gcgcgaggag cgcatcgagg cctggagccg cctcgcccgc gacctcgacg
    10621 ttcacaagct cgagcgcatg acgcgcgaga tcggcctcgg cgaggtggtc gacaccgccg
    10681 ccgcgctgct cgacggccag gagcgcgggc gcatcgtggt cgatgtcgat cgctgaaccc
    10741 gaccataaac acatcgacgg aggaaacttc caatgaccga aaccatcagc cgcttcaagg
    10801 tgcccgaact cgccgacctt gccgaggacg tgcgcgagcg catcctcgcc gtgcaggaga
    10861 aggcgggctt cgtgcccaac gtgttcctga tgctcgccca ccgcccggcc gaattccgcg
    10921 ccttcttcga ctaccacgac gccctgatgg agaaggacgt cggcctcagc aaggccgagc
    10981 gcgagatgat cgtcgtcgcc acctcggctg ccggccagtg cctgtactgc gtgatcgccc
    11041 atggcgccat cctgcgcatc cgcgcgaaga acccgcgcct cgccgaccag ctcgccacca
    11101 atcaccgcaa ggccgagatc agcccgcgcc agcgcgcgat gctcgacttc gccatcaagc
    11161 tcagcacgcg tggcgccgag gtcgacgagg ccgacctcgc ggtcctgcgc gcgcatggct
    11221 tcgacgacga ggcgatctgg gacatcggcg cgatcaccgc cttcttcagc atgagcaacc
    11281 ggctggtgca tctgaccggt acgccgccca acgagccctt ctacctgatg gggagggtgc
    11341 cgaaggcgtg aggtgcgagg tgcgaggtgc gtgcgggctg gagccgcgtc ggatagggag
    11401 ttcgtccggc gcggtgcaat tgcttacaaa cggaccgggc cttgtccgcg cgcaggcatt
    11461 gaatgcggcc gccgacctgc gtaagatcgc gggcatggtt gtaatcccgg caatctggag
    11521 gcgttctgga caggggttcg attcccctca cctccaccca agtgcattcg ggagtgtgct
    11581 tgggtggggg tgtaccggtt tcgacagggc gggcaaaggt gagcaggcaa cccgagaggc
    11641 gacggacgta atccgcgcaa atccataaac gccaacgatg agcgtttcgc actggccgct
    11701 taaggcctat gccgaggctg cttgagcctt gttaccaaag aaaagccggt ggggacttcg
    11761 gtccccatcg tcacgtctac gcggtccgca tttccctgta ttcggcgacg ttgcgcgcgt
    11821 cgaggctgcg ttttctccgg aacttcacga agaacgaccg cccggcgggc ggccgttgcg
    11881 ggcgcggctt cagcgcgtgg gagaccggcg tcgcattgcc ccaaggccgg cgagtccgag
    11941 ggcgagcagc gcgagccctc ccggctcggg ca
//
LOCUS       DAOQBS010000097        11955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4477_length_11955_cov_12.287563, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000097 DAOQBS010000000
VERSION     DAOQBS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11955
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4477_length_11955_cov_12.287563"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKN25_11045"
     CDS             complement(<1..245)
                     /locus_tag="PKN25_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91478.1"
                     /translation="MPLETTRLLNIGLRFTTLGTRFLFIFVLAKYLDPASVGYYGIFT
                     ATVGYCLYFVGLDFYTYLTREILTKPLNQRGQLIKAQA"
     gene            complement(334..1422)
                     /locus_tag="PKN25_11050"
     CDS             complement(334..1422)
                     /locus_tag="PKN25_11050"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNV91479.1"
                     /translation="MLTQLPVLTKTAVLAVVPLLLTLAVIQVLIKPARRIGLVDRPGG
                     RKEHDGEIPLIGGLGMAIALILSVQLAEPQTLIGLNVLGGIALLVVVGVLDDFLDIRA
                     KTKLLVQAIAAMLIVLPSGVYITHVGDILGIGNISLGPLALPFTVFAVVGLINAVNMA
                     DGADGLAAGLTSTTVVWFIACALVVGNQSMALDLVILLAVTVGFLCYNMRTPLRHKAA
                     VFMGDAGSMMLGALLAWFAIQLPHNSTAVPPPPPFAILWMLGLPVLDTVVLMIRRMLQ
                     GRSPFSAGRDHMHHIWMHAGFTPGETTLLIMGLNAVLGLVGFSGWILGLPEWVLAAGY
                     FAIFALHSMLATHAWKVSKWLKRHAHLA"
     gene            complement(1452..>3558)
                     /locus_tag="PKN25_11055"
     CDS             complement(1452..>3558)
                     /locus_tag="PKN25_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HNV91480.1"
                     /translation="NNQKHDSPFNVGVVYRYASGLDLTAGLERGNTLMLGLTLHGSLA
                     DLTVPKLLDPSYPRPLSTYPAGPTDWARMSAEFTEQTRWPVREVRRQGTDLQLVVEEP
                     YTIYLRDRIDRAVAVLHRHAPADVRRFVFSFHERGLPLTDWVVLRDEWVARQTRYRAH
                     AEQFEAVVAAEHWPAHSQEKLWVGPAERFTTGLEPSYSQSLGGPDGFILFQLGITAPT
                     ELRLADDTWLSGRANLGLIDNYDKFEYDGPSNLPRVRTDIRKYVTESDLTLPTLQLNH
                     VRALGTNQYASLYGGYLEPMFAGIGGEWLYRPWNSHFAFGIDLNHVRQREFDQHFGLR
                     DYDVTTGHATLYWHTGWENTLVKLSAGQYLAGDRGVTVDIARQFDNGVKMGVFATKTD
                     VSAEEFGEGSFDKGIYLSIPFDAMLPFSSARTADIIWRPLTRDGGAMLSRTLNLFDLT
                     RLRDRRVGRYAPSGSQAWLALGDDREDGRSIWGELGHTGGWLADKIFSPEAVRPLLLG
                     AGLTGLSATLDRPLADWADENQGGNWDTLGEAASLIPFGLAAGAGALWMGLADDAASD
                     AARTSLLAAGWTLGAQLATKYAVNRARPEAGLGPHEFRGPGNGGSDSSFPSIHMGVAF
                     ALATPFAQKYDAPWLYGLAAATGFGRIQERQHFFSDVVAGSLMGYGIASLLVDEQRSR
                     DGPVITLGADRSLQAHWMF"
     assembly_gap    3559..3658
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3659..4460)
                     /locus_tag="PKN25_11060"
     CDS             complement(<3659..4460)
                     /locus_tag="PKN25_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HNV91481.1"
                     /translation="MTRPLPAATTLARPIRASRLCLAVAQVCIVLGMPTAAGNAQAGG
                     GATASVASAQTAIRAGERLSDWLLRNPPGAGDDPAALAWQVPSARQAQAELKQALLAQ
                     LWSARDLQADETTRQRLADWLRSLPVTGRVPLAIVDARWLQANPDQDPILAPGHALVL
                     PQRPGTVTLLADDGRPCAVAHRPGTQARGYLQACIGGAASEADMAWIVQPDGRVQRFG
                     IATWNAEPQSEPAPGAWIWAPRRSAAWPEDFSDRLATFLATQSPTPIAA"
     gene            complement(4465..5145)
                     /locus_tag="PKN25_11065"
     CDS             complement(4465..5145)
                     /locus_tag="PKN25_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HNV91482.1"
                     /translation="MLAILACAACSTDSTNISRTFELAYRGGSLADRAAPNPAYRYLR
                     TVVDGRSALLVLGYTDPDPATPDAPPIEVWYSAEGEVVRLQQGRVVGTSGLSTDWRST
                     RAPDAPTWGSLVASAAQDRAAGQALATWQRTRDESAYRSGVIDRISVHAIAAPRGTEL
                     AGLAPESLQWFEERAEPIVPPAGTPGAVRLTARYALRVDSGATEVVYTEHCLGPELCL
                     SLQRWPAR"
     gene            complement(5436..5744)
                     /locus_tag="PKN25_11070"
     CDS             complement(5436..5744)
                     /locus_tag="PKN25_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91483.1"
                     /translation="MKKTALIAAILAFSTAPVLAQTAPRPGTGAPAQAAPAPAQAGAT
                     GAQAGAAGSGAGAAGASAAAVGGLTAGTVAAVAVGVAAVAAAASSDSSSGTVAPSGTR
                     "
     gene            5988..8399
                     /locus_tag="PKN25_11075"
     CDS             5988..8399
                     /locus_tag="PKN25_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNV91484.1"
                     /translation="MNTTEHCNRLRSAAKALILGLALACSSVTMAQNAPAAAGFAAAG
                     GAPAAVGSGGLGMGAPALSGGAPGAAVGVASGAAASAGAADGFETQAAPTVIGGEGLS
                     PSQDAALDQAVTVDADGNDRVGGTVRAARVLNDFQQFVARSTGQVLPLYGSGFFAGSR
                     VFNSPTAPVADDYVLGPGDQVLVRIWGAFESQTRAQIDRSGMITLPTIGPVSLAGVRI
                     ANAVQVIENQVGRIYRDVSVSVSLDRVRGITVFVVGQARRPGTYTVSGNSTLIGALFQ
                     SGGPGANGSLRRVQVKRDNQVITEIDLYRFLANGDTSADIRLVDGDVIVIPPAHGYVA
                     LTGQVKAPAIYELKDRADTLRSVLTVSGGLPVVADPRLAFVERLDPSADQPRSVFEVS
                     LQPGQPDFVLKSGDLVAVQPILAEFANAVTLRGGVSAPVRLPYRAGMRISDLIPDKAT
                     LINRYVVDNQNRSLLDRGSFVGDVGNLFVDINMDYAVVERLERPQMALKLIPFSLNGL
                     FADPNGPDNLRLQAGDTISIFTAGDVRVPVSRRRVVMRVEGEVNRPGVYVAEPGETLV
                     NIIEKAGGPTADAYLFGAEFYRESVRRSQQANLDKLVQRLEQQAVAESARVSANIIGD
                     AQAVAQAQAQLRAEREARGRFLARMRTLKSSGRMSLGLPADEPSFAQIPGFRLENGDR
                     LVIPNRPDFVQVFGAVNTESALLWRPSRTVSDYLEQAGMSREGDRSAAFVLRADGTVV
                     AETGSWFSSVMGTTVLPGDIIVIPELIDRESGWTAFARIAKDWTQIFANLGLGVAAVR
                     SIEND"
     gene            8403..9632
                     /locus_tag="PKN25_11080"
     CDS             8403..9632
                     /locus_tag="PKN25_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNV91485.1"
                     /translation="MTMNESTMQKAAIENPQGQMVVVEDDDEISLLDLAIVLAKFKKL
                     ILGLPVLVGALTVGATLLMTPIFTATTAILPPQQSQSTASALLGQLGGLAGIAGAAAG
                     IKNPSDLYVGMLKSRTVADAMIARFDLVNYYEDEFAEDARKSLENVSSFTAGKDGIIT
                     ISVDDKDPELAAKMANAYVEELNKLTEVLAVTEASQKRLFFERQMVDARDRLVAAEIE
                     ARSAMERGGLASIDAQGQAMIEVTARLRGQISVKEVEIGAMRAFAAEENPRLKAAQQE
                     LLALQTELARIEGASALRDTQVGGESSAGATNLQLLRNVKYYETLYQMLAQQFELAKI
                     EEAKDSALIQVLDTAIPPERKSKPKRALIVILAVLAAGFVAVLIAFMKEAAQRAAEDP
                     ESAERMQLFKKYMSWRA"
     assembly_gap    9674..9773
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9876..10241
                     /locus_tag="PKN25_11085"
     CDS             9876..10241
                     /locus_tag="PKN25_11085"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNV91486.1"
                     /translation="MRPPAKGLPFSRRVRFAWAGVRDAWRLEASFRSQCLAALAALVA
                     LAVLRPGWLWSAIVVVMIALVLAAELINTALEAALDGLHPEHARFVQVAKDCAAAAVL
                     VLSGASVVAFACMLADLYG"
     gene            complement(10263..11657)
                     /locus_tag="PKN25_11090"
     CDS             complement(10263..11657)
                     /locus_tag="PKN25_11090"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNV91487.1"
                     /translation="MLRATARPLHGFQAYTSALTRLADVLCVIGAGWVAYWLRFGDSQ
                     ISSGSYTSALVVGALLVLIIHPVFGIYRSWRGSLRLGLIAKLVAAYMMVGAAITTLLF
                     FTQSGGIFSRLWLAYWIVGAAVASISVRLVAYPLLSRIRSHGRNRRQVLLIGDAHSCA
                     TALHHLKAERTAGFDPGRLILIGGDPAFEMQGIAYEPYARGKLIEHDEDEVWICLPLS
                     QGETVKEIQSALSLSTANVRYMPDMRDFRLINHAISDVAGLYLLDLSCTPMTGFNTLV
                     KAVEDRVISALILLLISPILISVAIGVRLSSPGPIFYRQERVSWNGKPFMMLKFRSMP
                     VDVEKNGVQWGGAGSAKKTTPFGAFIRRTSLDELPQFINVLKGDMSIVGPRPERTVFV
                     DQFKHEIPGYMQKHMVKAGITGWAQINGWRGDTDLQRRIECDLWYIENWSLWLDLKII
                     FLTVFKGFVNPNAY"
BASE COUNT         1995 a         3657 c         4163 g         1940 t
ORIGIN      
        1 gcttgcgctt taattagctg tccgcgttga ttaaggggct tagttaggat ctctcgtgtt
       61 aaatacgtat agaaatccaa tccgacgaaa tagaggcagt agccgaccgt tgctgtgaaa
      121 attccgtagt agccgaccga tgcggggtcc agatatttcg caagcacaaa aataaataga
      181 aagcgcgtgc cgagcgttgt aaaacgtaag ccgatgttga gaaggcgtgt ggtttcaaga
      241 ggcatgcgtt ggggcttggg gtttgtttgg aaaataacga aaaatttcgc tatttgatct
      301 gcgatcagaa aattagcgat aaatgggggc tttttaagcg aggtgtgcgt gccgcttcag
      361 ccatttcgaa accttccaag catgcgttgc gagcatcgag tgcagggcga atatggcaaa
      421 ataccccgca gccagtaccc attcaggtaa ccccagaatc cagcccgaaa accctactaa
      481 tccgagcacc gcattcagcc ccatgatcag caacgtcgtt tccccgggag tgaaccccgc
      541 atgcatccag atgtgatgca tgtgatcccg cccggcgctg aacgggctgc gtccctgcag
      601 catgcggcgg atcatcagca ccacggtgtc gagcactggc aaccccagca tccacaggat
      661 cgcgaagggc ggaggtggcg gtacggcggt gctgttgtgc gggagctgga tcgcgaacca
      721 tgccagcagc gcacccagca tcatgcttcc ggcgtcgccc atgaacacgg ccgccttgtg
      781 gcgcagtggg gtgcgcatgt tgtagcagag aaagccgacc gtgacggcga gcaggatgac
      841 gaggtcgagg gccatcgact ggttgccgac gacgagggcg caggcgatga accagacgac
      901 ggtggtcgag gtgaggccgg ccgcgaggcc gtcggcgccg tcggccatgt tgaccgcgtt
      961 gatgaggccg acgaccgcga atacggtgaa gggcagggcc agggggccga ggctgatgtt
     1021 cccgattccc aggatatcgc ccacatgcgt gatgtagacg ccgctcggca gcacgatgag
     1081 catggctgcg atggcctgga cgaggagctt ggtcttggcg cggatgtcga ggaagtcgtc
     1141 gagtacgccg accacgacca gcagggcgat gccgccgagg acgttgaggc cgatcagggt
     1201 ctggggttcg gcgagctgta cgctgaggat gagcgcgatt gccatgccga ggccgccgat
     1261 gagggggatt tcgccgtcgt gctccttgcg gccgccgggg cggtctacca ggccgatgcg
     1321 ccgggcaggc ttgatcagca cctggatcac ggccagggtc agcagcagcg gcacgacggc
     1381 gagcactgcg gttttggtga ggacggggag ctgggtcagc atcgtgttca cagaaaacgg
     1441 ttgggctcgg cttagaacat ccagtgcgcc tgcaggctgc gatcggcccc gagggtgatc
     1501 accggcccgt cgcggctgcg ctgttcgtcc accagcagcg acgcgatgcc gtagcccatc
     1561 aggctgccgg cgaccacgtc cgagaagaaa tgctggcgtt cctggatgcg accgaagccg
     1621 gtggcggcgg cgaggccata cagccacggc gcatcatact tttgtgcaaa cggggtggcg
     1681 agtgcgaatg ctacgcccat gtgaatcgag ggaaagctgg aatccgaacc gccgttgccg
     1741 gggccgcgga attcgtgtgg gccgaggccg gcttcggggc gggcgcggtt gaccgcgtac
     1801 ttggtggcga gctgggcgcc gagcgtccag ccggcggcga gcaggctggt gcgcgcagcg
     1861 tcgcttgccg cgtcgtcggc taggcccatc cacagcgcgc cggcgcccgc ggcgaggccg
     1921 aaggggatga ggctggcggc ctcgccgagc gtgtcccagt tacccccctg attttcatcg
     1981 gcccagtcgg cgagggggcg gtcgagcgtg gccgagaggc cggtgagtcc ggcgccgagc
     2041 agcagggggc gcacggcctc gggcgagaag atcttgtcgg ccagccagcc accggtgtgg
     2101 ccgagctcgc cccagatcga gcggccgtcc tcgcggtcgt cgcccagcgc cagccaggcc
     2161 tgcgagccgg agggggcgta gcggccgaca cggcggtcgc gcaggcgggt gaggtcgaag
     2221 aggttgagcg tgcgcgacag catggcgccg ccgtcgcggg tgagcgggcg ccagatgatg
     2281 tcggccgtgc gtgcactgga gaagggcagc atggcgtcga aggggatgct gaggtagatg
     2341 cccttgtcga agctgccctc gccgaactcc tcggccgata cgtccgtctt ggtggcgaag
     2401 acgcccatct tgacgccgtt gtcgaactgg cgcgcgatgt ccacggtgac gccgcggtcg
     2461 ccggcgaggt actggccggc gctgagcttg accagcgtgt tctcccagcc ggtgtgccag
     2521 tacagggtgg cgtggccggt ggtgacgtcg tagtcgcgca ggccgaagtg ctggtcgaac
     2581 tcgcgctggc gcacgtggtt gaggtcgatg ccgaaggcga agtggctgtt ccacgggcgg
     2641 tagagccatt cgccgccgat gccggcgaac atcggctcga ggtagccgcc gtagaggctg
     2701 gcgtactggt tggtgccgag cgcgcgcacg tggttgagct gcagcgtggg cagggtgaga
     2761 tccgactcgg tgacgtactt gcggatgtcg gtgcgcacgc gcggcaggtt gctggggccg
     2821 tcgtattcga acttgtcgta gttgtcgatg aggcccaggt tggcacggcc cgacagccag
     2881 gtgtcgtcgg cgaggcgcag ctcggtgggc gcggtgatgc cgagctggaa caggatgaag
     2941 ccgtccgggc cgcccagact ctggctgtag ctgggctcga gcccggtggt gaagcgttcg
     3001 gcggggccga cccagagctt ttcctggctg tgcgcgggcc agtgctcggc ggcgaccacg
     3061 gcctcgaact gctcggcatg ggcgcggtag cgggtctgcc gggccaccca ttcgtcgcgc
     3121 agcacgaccc agtcggtgag cggcaggccg cgttcgtgga agctgaacac gaagcgacgg
     3181 acgtcggcgg gggcgtggcg gtgcagcacg gcgacggcgc ggtcgatgcg gtcgcgcagg
     3241 tagatggtgt agggctcttc gacgacgagc tgcaggtcgg tgccctggcg gcgcacctcg
     3301 cgtaccggcc agcgcgtctg ctcggtgaac tcggcgctca tgcgcgccca gtcggtggga
     3361 ccggcggggt aggtggagag cggcctgggg tagctggggt cgagcagctt gggcacggtg
     3421 aggtcggcca ggctaccgtg cagcgtgagg cccagcatca gcgtgttgcc gcgctcgagg
     3481 ccggcggtga gatcgagacc ggaggcgtag cggtagacga cgccgacgtt gaaggggctg
     3541 tcgtgcttct ggttgttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
     3661 gccgcgatcg gcgtggggga ctgcgtcgcc aggaaggtgg cgaggcggtc ggagaaatcc
     3721 tcgggccagg cggcgctacg gcgcggcgcc cagatccagg cgccgggagc cggttccgat
     3781 tgcggttcgg cgttccatgt ggcgattccg aaccgctgca cgcgaccatc tggttgcacg
     3841 atccaggcca tgtcggcctc gctggccgct ccgccgatgc aggcctgcag gtagccgcgg
     3901 gcctgggtgc cgggccggtg cgcgacagca caagggcggc cgtcgtcggc gagcaaggtg
     3961 acggttccgg ggcgctgtgg cagcacgagc gcgtggccgg gggcgaggat cggatcctgg
     4021 tccggattgg cctgcagcca gcgtgcgtcg acgatcgcca gcggcacgcg gccggtgacg
     4081 ggcaggctgc gcagccagtc ggcgaggcgc tggcgggtgg tctcgtcggc ctgcaggtca
     4141 cgtgcagacc acagctgggc gagcaaggcc tgcttgagct ccgcctgggc ctggcgggcg
     4201 cttggcactt gccaggcgag tgcagcgggg tcgtcgccag cgccgggcgg gttgcgcagc
     4261 agccagtcgc tgaggcgttc gcccgcgcgg atggccgttt gtgcggacgc cacggacgcg
     4321 gtcgcgccgc cgccggcctg ggcgttgccg gccgcggtcg gcatccccag cacgatgcag
     4381 acctgcgcca cggcgaggca cagacgtgat gcccggatgg ggcgtgcgag cgtggtcgcg
     4441 gcgggaagcg ggcgcgtcat cgagtcaccg tgccggccag cgctgcagcg agaggcagag
     4501 ctccgggccg aggcagtgtt cggtgtagac gacctcggtc gcgccgctgt cgacgcgcag
     4561 ggcgtagcgc gcggtgaggc ggacggcgcc cggcgtgccg gcgggcggga cgatcggttc
     4621 ggcgcgctct tcgaaccatt gcaggctttc gggggcgagg ccggcgagct cggtgccgcg
     4681 cggggcggcg atggcatgca cgctgatgcg gtcgatgacc cccgagcggt aggcggattc
     4741 gtcgcgggtg cgttgccagg tggcgagcgc ttggccggca gctcggtcct gagctgcgct
     4801 cgcgaccagg ctgccccagg tgggggcgtc tggtgcgcgg gtgctgcgcc agtcggtgct
     4861 gagcccgctg gtgccgacga cgcgcccctg ctgcaggcgc acgacctcgc cctccgcgct
     4921 ataccagacc tcgatcggag gcgcgtcggg cgtcgctgga tcgggatcgg tgtagcccag
     4981 cacgagcagc gcgctgcgac cgtcgaccac cgtgcgcagg tagcgatagg ccggattcgg
     5041 cgcagcccgg tccgccaagg agccgccgcg gtaggcgagc tcgaaggtgc gcgagatgtt
     5101 ggtcgagtcg gtgctgcagg ccgcgcaggc gagaatcgcc agcacggcca gtcgctctgg
     5161 taaagcgcgc gacatcggaa cagaaggtac ggcgttcatc aaacgaaaag cccccaaagg
     5221 gtgacccccg gggggcttgt gcttcgagtc gggtggtggt cgcgcgcagc tgttcgcggc
     5281 ttgcgccgct cctacggggc gcttgggcct tggcgaggtt cgcggcttgc gccgctccta
     5341 caaggcgctg gggccgtggc tttcgtgtag gagcggcgca agccgcgaat tttcagctgt
     5401 tgcgaacgat cgacctgaca acccgaggcg agcgatcaac gcgtgccgga cggtgcaacg
     5461 gtgcccgagc tgctgtcgct gctcgccgcc gcggccacgg ccgccacgcc gactgcgacg
     5521 gcggccaccg tgcctgcggt caaaccaccc accgcagccg cgctggcacc ggcggcgccc
     5581 gctccgctac ccgcagcacc ggcttgcgca ccggtcgcac ccgcttgggc cggcgcaggg
     5641 gctgcctggg caggagcgcc cgtgccgggg cgcggggcgg tttgcgccag cacgggcgcg
     5701 gtggagaagg caaggatcgc ggcgatcaga gcggtttttt tcatggaaac tcctgtcgag
     5761 gaaagcgcga ccggctggcc gagcgctgga aaagtttgtt tcggacgcaa ctattctagc
     5821 ttgtgccgac cgtggttgaa acatgaacaa gtccttgaag gagaagaata cgacaatcga
     5881 ggccgcccga ttccggctgc cccgccaagc cgtgaccgag cgcagccagt cagccgactt
     5941 tttccagcta caatccacgc cctcaagcgt ccttatgttg tcagaaaatg aataccaccg
     6001 aacattgcaa ccgcctgcgg agcgctgcga aggcgctgat cctgggcttg gcgctggctt
     6061 gctcgtccgt gacgatggcg cagaacgccc ccgcagcagc gggcttcgcg gcggcgggcg
     6121 gtgctccggc tgccgtgggg agcggcgggc tgggcatggg ggcccctgcc ttgtccggtg
     6181 gcgcgccggg tgcggccgtc ggtgtcgcca gcggtgctgc ggcatctgca ggtgccgcgg
     6241 acggattcga gacccaggct gctccgaccg tgatcggcgg tgaaggcctg agcccctccc
     6301 aggatgccgc gctcgaccag gcggtgacgg tcgacgccga cggcaacgac cgcgtgggcg
     6361 gcacggtgcg cgccgcgcgg gtgctcaacg acttccagca attcgtggcg cgcagcaccg
     6421 ggcaggtgct gccgctgtac ggttcgggct tcttcgcggg ctcgcgagtg ttcaacagcc
     6481 ccaccgcgcc ggtcgccgac gactacgtgc tcggcccggg cgaccaggtg ctggtgcgga
     6541 tctggggcgc cttcgagtcg cagacccggg cgcagatcga ccgcagcggc atgatcacgc
     6601 tgccgaccat cggcccggtg agcctggccg gggtgcggat cgccaatgcg gtgcaggtga
     6661 tcgagaacca ggtggggcgc atctaccgcg acgtgagcgt cagcgtgagc ctggaccggg
     6721 tgcgcggcat caccgtgttc gtggtgggcc aggcgcggcg tccgggcacc tacacggtgt
     6781 cgggcaattc gaccctgatc ggcgcgctgt tccagagtgg cggcccgggg gccaatggtt
     6841 cgctgcgccg cgtgcaggtc aagcgcgaca accaggtgat caccgagatc gacctgtacc
     6901 gcttcctcgc caacggcgac acctcggccg acatccgcct ggtggacggc gacgtgatcg
     6961 tgattccgcc ggcgcacggc tacgtggcgt tgaccggcca ggtgaaggcg cctgcgatct
     7021 acgagctcaa ggaccgtgcg gacacgctgc gcagcgtgct gacggtgtcg ggcgggctgc
     7081 ccgtcgtggc cgacccgcgc ctggccttcg tcgagcgcct ggacccgagc gcggatcagc
     7141 cgcgctcggt gttcgaggtg tcgctgcagc cagggcagcc cgacttcgtg ctgaaatccg
     7201 gcgacctggt cgcggtgcag cccatcctgg ccgagttcgc caatgcggtg acgctgcgcg
     7261 gtggggtgag cgcaccggtg cgcctgccct accgcgccgg catgcggatc tcggacctga
     7321 ttccggacaa ggccaccctg atcaaccgct acgtggtgga caaccagaac cgcagcctgc
     7381 tcgatcgcgg cagcttcgtc ggcgatgtcg gcaacctctt cgtcgacatc aacatggatt
     7441 acgcggtggt cgaacggctc gagcgcccgc agatggcgct caagctgatt ccgttcagcc
     7501 ttaacggcct cttcgccgac cccaacggcc cggacaacct gcgcctgcag gccggcgaca
     7561 cgatctcgat cttcaccgcg ggcgacgtgc gcgtgccggt gagccgccgc cgcgtggtga
     7621 tgcgggtgga gggcgaggtg aaccgccccg gggtgtatgt ggccgagccg ggcgagaccc
     7681 tggtgaacat catcgagaag gcgggcggac ccaccgcgga cgcctacctg ttcggcgccg
     7741 agttctatcg cgagtcggtg cgcaggtcgc agcaggccaa cctcgacaag ctggtgcagc
     7801 gcctggagca gcaggcagtt gccgagtcgg cgcgggtttc cgccaacatc atcggggacg
     7861 cccaggccgt ggcccaggcg caggcgcagc tgcgcgccga gcgcgaggcg cgtgggcgtt
     7921 tcctcgcccg catgcgcacg ctgaagtcct cgggccggat gtcgctcggc ctgccggcgg
     7981 acgagccgag cttcgcgcag atccccggct tccggctgga gaacggcgat cgcctggtga
     8041 tcccgaatcg gcccgatttc gtgcaggtgt tcggtgcggt caataccgag tctgccctgc
     8101 tgtggcggcc cagccgcacc gtttccgact acctggagca ggccggcatg agccgcgagg
     8161 gcgaccgtag cgcggccttc gtgctgcgcg ccgacggcac ggtggtggcc gaaacgggca
     8221 gttggttcag cagcgtgatg gggacgacgg tgctgccggg cgacatcatc gtgattccgg
     8281 agctgatcga tcgcgagtcg ggctggacgg ccttcgcgcg catcgccaag gactggacgc
     8341 agatcttcgc caacctcggc ctcggtgtgg ccgcggtgcg ctcgatcgag aacgactgag
     8401 cgatgaccat gaacgaaagc acgatgcaga aggcggcgat cgagaacccg cagggccaaa
     8461 tggtggtggt cgaggacgac gacgagatct ctctgctcga tctcgcgatc gtgctcgcca
     8521 agttcaagaa gctgatcctc ggcctgccgg tgctggtggg ggccttgacg gtgggcgcga
     8581 cgcttctgat gacgccgatc ttcaccgcga ccacggccat cctgccgccg cagcagtcgc
     8641 agtcgaccgc ctcggcgctg ctcggccagc tcggcgggct cgccggcatt gccggcgcag
     8701 cggccgggat caagaacccg agcgacctct acgtcggcat gctgaagagc cgcaccgtgg
     8761 ccgatgcgat gatcgcgcgc ttcgacctgg tgaactacta cgaggatgag tttgcggagg
     8821 acgcgcgcaa gtcgctggag aatgtatcca gcttcaccgc cggcaaggac ggcatcatca
     8881 ccatctcggt cgatgacaag gaccccgagc tcgccgcgaa gatggccaac gcctacgtgg
     8941 aagagctgaa caagctgacc gaggtgctgg cggtgaccga ggcctcgcag aagcgcctct
     9001 tcttcgagcg ccagatggtc gacgcgcgcg accgcctcgt ggcggccgag atcgaggcgc
     9061 gctcggcgat ggagcggggc ggcctggcga gcatcgacgc ccagggccag gcgatgatcg
     9121 aggtgacggc gcggctgcgc gggcagatct cggtgaagga ggtcgagatc ggcgccatgc
     9181 gcgccttcgc cgccgaggaa aacccccgcc tcaaggctgc gcagcaggag ctgcttgcgc
     9241 tgcagaccga gctcgcacga atcgagggcg cgagcgcgct gcgcgacacc caggtcggtg
     9301 gcgaatcgag cgccggcgcg accaacctgc agttgctgcg caacgtgaag tactacgaga
     9361 cgctgtacca gatgctggcg cagcagttcg agctcgccaa gatcgaggag gccaaggaca
     9421 gcgcgctgat ccaggtgctg gacaccgcca tcccgcccga gcgcaagtcc aagcccaagc
     9481 gcgccctgat cgtgatcctc gccgtgctcg ccgccggctt cgtcgccgtg ctgatcgcct
     9541 tcatgaagga agccgcccag cgtgccgccg aagaccccga aagcgcggag cgcatgcagt
     9601 tgttcaagaa atatatgtcc tggcgggcgt gagaaagtct tgatacgagc ccgccccgcg
     9661 caccacgctt cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccccgta
     9781 ggagcgacgc aagtcgcgat cacagccgct gagatcgcga ctcgcgtcgc tcctacaaca
     9841 ccgtctgact ctcgtctcgt cgctggagtg aagagatgcg acctcccgcc aagggccttc
     9901 ccttctcccg ccgcgttcgc ttcgcctggg ctggcgtgcg cgacgcctgg cggctggaag
     9961 ccagcttccg cagccaatgc ctcgcggcgc tcgccgcgct cgtcgcgctc gcggtgctgc
    10021 gccccggctg gctatggtcc gccatcgtcg tcgtgatgat cgccctcgtg ctggctgccg
    10081 agctcatcaa caccgcgctc gaagccgctc tcgacggcct ccatcccgag cacgcgcgct
    10141 tcgtccaggt cgccaaggac tgcgccgcgg ctgccgtcct ggttctcagc ggggcctcgg
    10201 ttgtcgcctt cgcgtgcatg ctggccgacc tgtacggata ggcaacggcc gggtgacccg
    10261 gctcaatacg cgttggggtt cacgaagccc ttgaacaccg tcaggaagat gatcttcagg
    10321 tcgagccaga gcgaccagtt ctcgatgtac cagagatcgc attcgatccg tcgctgcaga
    10381 tccgtgtcgc cgcgccagcc attgatctgt gcccatcccg tgatgcccgc cttcaccatg
    10441 tgcttctgca tgtagcccgg gatctcgtgc ttgaactgat ccacgaacac ggtgcgctcg
    10501 ggacgcggtc cgacaatgga catatcgcct ttgagtacgt tgatgaactg aggcaattcg
    10561 tcgagactgg ttcggcggat gaacgcgccg aagggcgtgg tcttcttggc gctgcccgcg
    10621 ccaccccatt ggacaccgtt tttctcaacg tccaccggca tggagcgaaa tttcagcatc
    10681 atgaacggct tgccgttcca gctcacgcgt tcctggcggt agaagatcgg accgggagag
    10741 ctgaggcgga caccgatagc cacggatatc aggatcgggc tgatcaacaa caggatcagc
    10801 gcggagatca cccggtcttc gaccgccttg acaagggtgt tgaagcccgt catcggcgta
    10861 cagctcaaat caagcaaata caggccggcg acatccgata ttgcatgatt gatcaagcgg
    10921 aaatcccgca tgtcaggcat gtagcgcacg ttcgcagtcg ataggctcag agccgattgg
    10981 atctccttta ccgtttcgcc ttgggacaga ggcaagcaga tccaaacctc gtcttcgtca
    11041 tgctctatca acttaccccg agcgtacggc tcataagcaa tcccttgcat ctcgaatgct
    11101 ggatcgcctc caatgaggat taatcggcca gggtcgaaac ccgctgtcct ctcggccttc
    11161 aaatgatgga gagctgttgc gcaagaatgg gcgtccccga tcagcagcac ctgtctccgg
    11221 tttcggccat ggctacggat tcggctcagc aacggatagg cgactagccg cacagagatg
    11281 gatgccaccg cagcgccaac aatccagtac gccagccaaa gtcgcgaaaa gatacctccc
    11341 gattgcgtaa aaaacagcag ggtggtgatc gcggcgccca ccatcatata ggccgccacc
    11401 agttttgcga tgaggccgag gcgcaggctt ccacgccaag atctgtagat cccgaaaact
    11461 ggatggatta ttagtacgag taaggcgcct acgacaagtg cgcttgtata gctgccagac
    11521 gagatctggg agtcgccaaa tcgcaaccag taagcaaccc aaccagcccc gatcacgcat
    11581 agaacgtccg cgagtcgcgt cagtgcgctt gtgtaggcct gaaagccatg aagcggtcgt
    11641 gcagtcgctc tcaacacttt ggaaagttcc tcctttggcc gagtcagtcg aatggcatga
    11701 agtctgctgc catcgcgcca cgattttgaa aatcaatcag gaaagataag ttgccaattt
    11761 tccaattagt agtgtcagtt atcgagtcca tgaaagccga ggaaattgaa tgactggcgc
    11821 tctggagctg cgctaacagt accttccgaa gtcctagaca gtcggcaact cgaaaacttc
    11881 aaggaaagtc ccgcagcctc gggttgttcg cgtccttcgc cgacagctgc ggcgcttcga
    11941 tcggccacga gatcc
//
LOCUS       DAOQBS010000098        11935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4494_length_11935_cov_15.687121, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000098 DAOQBS010000000
VERSION     DAOQBS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11935
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4494_length_11935_cov_15.687121"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKN25_11095"
     CDS             complement(<1..677)
                     /locus_tag="PKN25_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV91488.1"
                     /translation="MKTSSQPLFRSAAAVALSALVLAACSGGKEQQGGAPGAGAPPQA
                     PAVTVMTVASGSVPMVTELPGRTTPYLIAELRPQVTGILTQRAFDEGSEVKAGQVLYR
                     IDDAPYQAAHDSAKAALARAEANAQVARIKAERHAGLVKIDAVSKQANDDTQAALKQA
                     QAEVAAAKAALDKARIDLDYTRLKSPISGRIGRSAVTPGALVTANQVQALATVQQLDP
                     IYVDLTQS"
     gene            876..1499
                     /locus_tag="PKN25_11100"
     CDS             876..1499
                     /locus_tag="PKN25_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV91489.1"
                     /translation="MQITDPTPHTTEASRATARRQQILAAAAQCFREHGFHGASIARI
                     SKIAGMSAGHIYHYFENKEAIIAAIVAQDLETLLTLTAELRAACNVRNALIERAAEGV
                     LDQLDPDSAALKVEIVAEAARNSHVAQIVREADATCRRELMVTIRAIRSAAGHDDTPE
                     ATADMVEVLAALFEGLLLRTIRNPELDRKHVAQRIQLAINDLLDQPG"
     gene            1628..2029
                     /locus_tag="PKN25_11105"
     CDS             1628..2029
                     /locus_tag="PKN25_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF995 domain-containing protein"
                     /protein_id="HNV91490.1"
                     /translation="MNRLLACAAAALLLSACASTEKELADKGMKPLDAAKVRTLLSGN
                     TATGTTASGSGFMIYNAPDGTTRGKSGNSSDTGKWDVTADGQHCNHWTSWRGGERRCA
                     RLYEADGEIRYFDGGNYAGRFKIEQGNPYKL"
     gene            complement(2103..3386)
                     /locus_tag="PKN25_11110"
     CDS             complement(2103..3386)
                     /locus_tag="PKN25_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNV91491.1"
                     /translation="MIGSVLIGLFLLALLAGMPLATGLGVASIAVLAMAGFDQLAVPT
                     NIYAGIAKYPLLAIPMFILAGMIFERSGVAIRLVRFVTAIVGEWTGSLAVVAVLVSML
                     LGGISGSGPADAAAVAAVMLPSMIARGYPKGFSAGLIAASGSTAIVIPPSVAFIVYSV
                     MVPAATVPALFAAGLFPGIVAALCLLVPAIVISRKYGFGRDYKAARPPLGKSFIEAIW
                     GLLAPVIILGGLRTGIFTPTEAAVVAVAYGIFVGMVVYRSISFRGLFRVLVEAGELSA
                     VVLMIIGIASVFAWAGNTLGIFDAAAKALVGLHSSEWLMLLSINLLLLAAGMFLDAIS
                     IFLILLPLLVPIAVAMGWDLVWFGVMMTINLAIGQFTPPMAISLMITSRIAGIGMEET
                     FKWVIWLVLAMGAGLTLMIVFPQLALWLPGKLGYL"
     gene            complement(3383..3868)
                     /locus_tag="PKN25_11115"
     CDS             complement(3383..3868)
                     /locus_tag="PKN25_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNV91492.1"
                     /translation="MNIESPPHDAESPASYTIEQILSGTVMGLIVLITFGNVLARYFT
                     SQSFAATEEFSIALLIVLSFLGSSAAFAFDRHIRVDFFVRKLPPGLRAFADWLSFAVT
                     ASLFAFIAFYAGKLTWDQYRFEETSPALGVPQWWYTVWMPVLAAALVLRLVLTRVRGR
                     A"
     gene            complement(3948..4979)
                     /locus_tag="PKN25_11120"
     CDS             complement(3948..4979)
                     /locus_tag="PKN25_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNV91493.1"
                     /translation="MQKRRFTALIAGLFASTALGFSMPATAQQYKDEYKLSTVLGEAF
                     PWGWGAKRWADLVAEKTEGRIKIKVYPGTSLVSGDQTKEFTALRQGIIDMAVGSTINW
                     SPQVKELNLFALPFLMPDHKAIDALTQGRVGKKMFDILAERDVVPLAWGENGFREVSN
                     SKKPIRTPEDVKGMKMRVVGSPLFLATFNALGANPTQMSWADAQPAMATGAVDGQENP
                     LAVFNAAKLHTVGQKNLTLWGYVADPLIFVVNKSVWNSWSEADRKAVSEAAQQAAKEE
                     IARARAGISAADDALLKEIEANGVAVVRLTDAERDAFRQATAGVYKEWAEKIGADLVK
                     QAEEDIAKR"
     gene            complement(5378..6358)
                     /locus_tag="PKN25_11125"
     CDS             complement(5378..6358)
                     /locus_tag="PKN25_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV91494.1"
                     /translation="MGAPGLIAAFTAGAATLAGGRALVHWGIRKGLAAPRVPHHTDPG
                     ALGLAFETLRIGTEHGKSLHAWFIPAPGSACDGSDHAASPGADLADGERDDARAEFES
                     APAVVVMHGWGGNAALMLPLARPLHEAGYAMLFVDARCHGASDDDSFASLPRFAEDAE
                     HAFAWLAAQPGVDPARIALLGHSVGAGAVLFAASRTPQVAAVVSVAAFSHPAAMMRRW
                     LAGKRIPEKPLGRYILDYVQKTIGHRFDDIAPVNTIARIRRPVLLVHGADDEVVPIDE
                     AMQIYAMRGDTPVELMTLSGDHESFVDLEHHVGRLVEFLGRVLAQGGARE"
     gene            complement(6371..6718)
                     /locus_tag="PKN25_11130"
     CDS             complement(6371..6718)
                     /locus_tag="PKN25_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNV91495.1"
                     /translation="MNTVVLEVRSLTDTLADAARAMKTGRGDAQVRIAFATPELLWKV
                     LSAKRWELLKALCAAGPVSIREAARRVGRDVKAVHGDVTALLDAGVLLRTEDGRIEFP
                     FDAVKVEFLLQAA"
     gene            complement(6699..6995)
                     /locus_tag="PKN25_11135"
     CDS             complement(6699..6995)
                     /locus_tag="PKN25_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HNV91496.1"
                     /translation="MKAIPLIRRRVVLAPDAFAEIAVWRVSEPVPPSGHPFKYRLAYV
                     VGGECVLRYDNERGKGDHRHYGDTEAEYVFSDPDQLTRDFGADIARWNHEHGRS"
     gene            complement(7044..7676)
                     /locus_tag="PKN25_11140"
     CDS             complement(7044..7676)
                     /locus_tag="PKN25_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HNV91497.1"
                     /translation="MTKPTDDMGDSRPAPAPHAEAEGAGARSPANAECETAEPVRKPP
                     RWRRWGVEIGIFVVIFFAVQAWMARDVPAGLAPDFATVAADGRGLSLAEWRAAHPGKA
                     VGVYFWADWCPICTAQQGTIDGVQADHPVLTVAMQSGGAAEVTGVLAERGLAWTTAID
                     ADGRIAEAYGLRGVPAFVIVDPAGKIRSVSLGYATGWGLRARLWWAGLQS"
     gene            complement(7673..8764)
                     /locus_tag="PKN25_11145"
     CDS             complement(7673..8764)
                     /locus_tag="PKN25_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNV91498.1"
                     /translation="MDTLTHALSGALVGRMLASRRPAAGTRAANAAPVPVWQMVVAGT
                     AAAIFPDLDFVLGWISELTYLRGHRGVTHSLILLPLWGVLIAWLLARFWQRGGRVAAG
                     WRSFYPVVCAAIFVHILGDLITQFGTMILAPFSDHRFGWGTSFIIDLPFTGILLAGLL
                     ASALWRSSRVPATAALVLLAGWVGVQATGRAEAIDAARAYAAAKGIAAVEVDAAPRPA
                     SPFNWTAIVFDGERYHYAHLNTRRSEPLVATEDDNFIRRFSAPYMPVAMAQWEVRERF
                     GTGSTRALAEQVWNAEDFAFYRWFAMFPVLDHAGEAGDGQACASFKDLRFLTPGRDAQ
                     PFIYGLCNAAGGWRLFEREAGGLRWIDPR"
     gene            complement(8840..10447)
                     /locus_tag="PKN25_11150"
     CDS             complement(8840..10447)
                     /locus_tag="PKN25_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV91499.1"
                     /translation="MHASETLLNTLRRPGVLPGSEGGVELIETHISWVLLAGEHAWKL
                     KKPLDLGFLDFSTPARRREACEAELRLNRRTVPEIYEAVVAVRGSPEAPRLDGGGEPF
                     DWLLRMRRFDQAGLFSRLLDEGRLAPALFDRLARHVAAFHAAAAVALPGGAFGDAAAV
                     HAPVRQNFAQMRERLDPGVHADLLAMLARVEAWAEAQYAALAPVFAVRLAEGRVRECH
                     GDLHLGNLVVLDGEPRLFDAIEFSAELRWTDVVADVAFLVMDLQARGRAALGWRFLNA
                     WLERCGDYAGLRVLPYYLSYRAMVRAKIAAIRASQLDGAARSESVDECRRYLALAEAQ
                     RAPGAPALLVACGVAGAGKTSQSQALVEAGGVIRVRADVERKRLAGLAAEATSGSGLG
                     GGLYTPEATARTYARLAVLARVVLEAGRPVLVDATFLKRAQRAAFAGLAQELGVPFAI
                     LAFDAPEDVLRARVRARLAAGGDASEADEAVLEAQLRSREGFAAEELARVLPIDTRSV
                     PDWRSLLPALSRLWPGAPRMDSRPSTH"
     gene            10567..11451
                     /locus_tag="PKN25_11155"
     CDS             10567..11451
                     /locus_tag="PKN25_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV91500.1"
                     /translation="MPATPFTALACPLDGAPLQRQGGSLRCADGHCFDLAAQGYVNLL
                     PVQNKRSRDPGDSKEMVAARRRFLEAGHYAPIAAAVARTVFEGAAPGQTLACLDAGCG
                     EGYYLRQLSAAAVVEARTLALAGLDISKWAVQAAAKRDLQDARSRAGADPSTAWIVGS
                     NAGLPVLPATLDRVLCMFGFPVYPEFARVLRPGGELLQVDAGPDHLRELREVIYPALK
                     PERPASRAAPPGFEHLASDTLRFQIHVDGAAQIADLLAMTPHLYRASAEGRARAAALV
                     ALTMTVDVRLARYRRQPG"
     gene            11602..11931
                     /locus_tag="PKN25_11160"
     CDS             11602..11931
                     /locus_tag="PKN25_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNV91501.1"
                     /translation="MRATTLIATLALTGGLIGAGAAIAPAFAQNAAPAATPAAATAPA
                     AQSNWMSPKDVQTKLEGAGYTDFREIERDKNKYEVKATDPQGQRVELDVDPVTGDVLK
                     TEVKRSK"
BASE COUNT         1950 a         4302 c         4084 g         1599 t
ORIGIN      
        1 gactgggtga ggtcgacgta gatcgggtcg agctgctgca cggtggcgag cgcctgcacc
       61 tggttggcgg tgaccagcgc gcccggcgtc accgccgagc gtccgatgcg cccggagatc
      121 ggcgacttga ggcgggtgta gtcgaggtcg atgcgcgcct tgtcgagcgc cgccttcgcc
      181 gcggcgacct cggcctgggc ctgcttcagc gcggcctggg tgtcgtcgtt ggcctgcttg
      241 ctgacagcgt cgatcttgac cagcccggcg tggcgttcgg ccttgatgcg cgcgacctgg
      301 gcattggcct cggcacgggc gagcgccgcc ttggcgctgt cgtgggcagc ctggtagggg
      361 gcgtcgtcga tgcggtagag cacctgcccg gccttgacct cgctgccctc gtcgaaggcg
      421 cgctgggtga ggatgccggt gacttgcggg cgcagctcgg cgatcagata gggcgtggtg
      481 cggccgggca gctcggtcac catcggcacg ctgccgctgg cgaccgtcat cacggtcacc
      541 gccggcgcct gcggcggcgc cccggcgccc ggcgcgccgc cctgttgttc cttgcctccg
      601 ctgcaggcgg ccaggaccag ggcgctcagc gccaccgcag ccgcggacct gaaaaggggt
      661 tgggagcttg tcttcattgg agcctcgtga acgcgagaaa cgacgacacc ggcgtgcgac
      721 tgttgatcgc tggccgacat cccgctcatt agaatggcta ttctagatcg aacgctcatt
      781 ctatgttatg gtttgctgca tcgcaagatt tttaaggaca tcgtcccgcc ccggtggttc
      841 atcgccggcg ggagcgaagg cctgaccgga gcagcatgca gatcacggac cccacgcccc
      901 acactaccga agcctcgcgc gccaccgccc gtcgccagca gatcctcgcc gccgccgcgc
      961 agtgcttccg cgagcacggc tttcatggcg ccagcatcgc acggatctcg aagatcgcgg
     1021 gcatgagcgc cggccatatc taccactact tcgagaacaa ggaggcgatc atcgccgcga
     1081 tcgtggcgca ggatcttgaa accctgctga ccctgaccgc cgagctgcgc gccgcctgca
     1141 acgtgcgcaa cgccttgatc gaacgcgccg ccgaaggcgt gctggatcag ctcgaccccg
     1201 acagcgcagc gctgaaggtg gagatcgtcg ccgaggccgc gcgcaactca cacgtggcgc
     1261 agatcgtccg cgaggccgac gccacctgtc ggcgcgaact gatggtcacc atccgcgcca
     1321 tccgcagcgc cgccggccac gacgacacgc cggaagcaac tgccgacatg gtcgaagtgc
     1381 tcgcagccct gttcgaaggc ctgctgctgc gcacgatccg caaccccgag ctcgaccgca
     1441 agcacgtggc gcagcgcatc cagctcgcca tcaacgatct gctcgaccag ccgggctgag
     1501 cttccggcac caggcatccc ctcggcttga cgaacgcatg accaagtcct agaccagtca
     1561 tagcggcgca ctgctctcgc gccgcgcaac ggcaaaggac cacaacaacg tcccgacgga
     1621 gacccaaatg aacagactgc tcgcctgcgc ggcggcggca ctgctgctga gtgcctgcgc
     1681 atccaccgag aaagaacttg ccgacaaggg catgaagccg ctcgacgcgg cgaaggtccg
     1741 caccctgctg tccggcaaca ctgcaaccgg caccaccgcg tccggcagcg gcttcatgat
     1801 ctacaacgcc cccgacggca ccacgcgcgg caagagcggc aacagcagcg acaccggcaa
     1861 gtgggacgtc accgccgacg gccagcactg caaccattgg acctcctggc gcggcggcga
     1921 gcggcgctgc gcgcggctgt acgaggcgga cggcgaaatc cgctatttcg acggcggcaa
     1981 ctacgctggc cgcttcaaga tcgagcaggg caatccctac aagctctgag aatcaatggg
     2041 gtcggactcg atgatcgtcc cggtcggcga ccgatcgagt ccaaccccac tgaatccggg
     2101 ccttacaggt agccgagctt ccccggcagc cacagcgcga gctgcgggaa cacgatcatc
     2161 agcgtcagcc cggcacccat cgccagcacc agccagatca cccacttgaa ggtctcctcc
     2221 atgccgatcc cggcgatgcg cgaggtgatc atcagggaga tcgccatcgg cggggtgaac
     2281 tggccgatgg cgaggttgat cgtcatcatc accccgaacc acaccaggtc ccagcccatc
     2341 gccaccgcga tcggcaccag cagcggcagc aggatcagga agatcgagat cgcgtcgagg
     2401 aacatccccg ccgcgagcag cagcaggttg atcgacagca gcatcagcca ttcactggag
     2461 tgcaggccga ccagcgcctt cgccgcggcg tcgaagatgc ccagcgtgtt gcccgcccag
     2521 gcgaataccg aggcgatgcc gatgatcatc agcaccaccg ccgacagctc gccggcctcg
     2581 accagcacgc ggaacaggcc cctgaagctg atgctgcgat acaccaccat gccgacgaag
     2641 atgccgtagg ccaccgccac caccgccgcc tcggtgggcg tgaagatgcc ggtgcgcagg
     2701 ccgccgagga tgatcaccgg cgccagcagg ccccagatcg cctcgatgaa gctcttgccg
     2761 agcggcgggc gcgcggcctt gtagtcgcgg ccgaagccgt acttgcggct gatcacgatc
     2821 gccggcacga gcaggcacag tgcggcgacg atgccgggga acaggccggc ggcgaacagc
     2881 gccggcacgg tcgccgccgg caccatcacg ctgtagacga tgaaggccac cgagggcggg
     2941 atcacgatcg cggtcgaacc cgaggcggcg atcaggcccg ccgagaagcc cttcgggtag
     3001 ccgcgcgcga tcatcgacgg cagcatcacc gccgccacgg cggccgcatc ggccgggccc
     3061 gaaccggaga tgccgccgag cagcatcgac accagcaccg ccaccaccgc cagcgagccg
     3121 gtccattcgc ccacgatcgc ggtgacgaag cgcaccaggc ggatggccac gccggagcgc
     3181 tcgaagatca tgccggcgag gatgaacatc gggatcgcca gcagcgggta cttggcgatg
     3241 ccggcgtaga tgttggtcgg caccgcgagc tggtcgaagc cggccatcgc gagcacggcg
     3301 atgctggcca cgcccaggcc ggtggcaagc ggcatgcccg cgagcagggc gagcaggaaa
     3361 aggccgatga gcacgctgcc gatcatgccc gcccccgcac ccgggtcagc accaggcgca
     3421 gcaccagcgc cgccgccagc accggcatcc acaccgtgta ccaccactgc ggcacgccca
     3481 gcgcgggcga ggtttcttca aaccggtact ggtcccaggt gagcttgccg gcgtagaagg
     3541 cgatgaaggc gaacaggctg gcggtgaccg cgaacgacag ccagtcggcg aacgcgcgca
     3601 agcccggtgg cagcttgcgg acgaagaaat cgacgcggat gtggcgatcg aaggcgaagg
     3661 cggccgagga acccaggaag gacagcacga tcagcagcgc gatcgagaac tcctcggtcg
     3721 cggcgaagga ctgcgaggtg aagtagcgcg ccagcacgtt gccgaaggtg atcagcacga
     3781 tcagccccat gacggtgccg gagaggatct gctcgatcgt gtaggaggcg ggggattcgg
     3841 cgtcgtgcgg gggtgattcg atattcatgg gttcagggtg ccttcaatgg aaaaaggccg
     3901 gacagcccga cttcggtact gtccggcccg cgcactgcgg gtgcggatca gcgcttggcg
     3961 atgtcttcct cggcctgctt gacgaggtcg gcgccgatct tctcggccca ctccttgtac
     4021 acgcccgcgg tggcctggcg gaaggcgtcg cgctcggcat cggtcaggcg caccacggcc
     4081 acgccgttgg cctcgatctc cttcagcagc gcgtcgtcgg ccgccgagat gccggcgcgc
     4141 gcgcgcgcga tctcttcctt cgcagcctgc tgcgcggcct cggacacggc cttgcggtcg
     4201 gcctcggacc acgagttcca cacggacttg ttcaccacga agatcagcgg gtcggcgacg
     4261 tagccccaca gggtcaggtt cttctgcccc acggtgtgca gcttggcggc gttgaacacc
     4321 gcgagcgggt tctcctggcc gtcgaccgcg ccggtcgcca tcgccggctg ggcgtcggcc
     4381 cagctcatct gcgtcgggtt ggcgccgagc gcgttgaagg tggcgaggaa gagcggggaa
     4441 ccgaccacgc gcatcttcat gcccttgacg tcctcgggcg tgcggatcgg cttcttcgag
     4501 ttggagacct cgcggaaacc gttctcgccc caggccagcg gcaccacgtc gcgctcggcg
     4561 aggatgtcga acatcttctt gccgacgcgg ccctgggtga gcgcgtcgat cgccttgtgg
     4621 tcgggcatca ggaagggcag cgcgaacagg ttgagctcct tgacctgcgg cgaccagttg
     4681 atcgtggaac cgaccgccat gtcgatgatg ccctggcgca gcgcggtgaa ttccttggtc
     4741 tggtcgcccg acaccagcga agtgcccgga tacaccttga tcttgatgcg gccttcggtc
     4801 ttctctgcga ccaggtcggc ccagcgcttg gcgccccagc cccacgggaa ggcctcgccg
     4861 agcaccgtgg agagcttgta ctcgtccttg tactgctggg ccgtggccgg catcgagaaa
     4921 ccgagcgcgg tcgaggcgaa caggccggcg atgagggcgg tgaagcggcg tttctgcatg
     4981 aggcgtctcc gtgttgtgct tgtttttccg gctgcgcggc acacggcgcg ggcctccgga
     5041 ggatgggaag gagggtcgat tctacaaacc ggaggccccc acggccagcg cggagacggc
     5101 gggccgatcg agtgtgggct tcgggcgcgg gacgcggccg ggggcggcgc ttcgtttcaa
     5161 ggccacaatt gcgacttgcg tggcgcagcc aggggcgtcg ggggagcggc gtcggatggg
     5221 cgtcgggagg gggcgtcggg cactcgccat gcgcgcggcg gttcattgac cgctgcgatc
     5281 gcgacttgcg tcgctcctac aaaaaccgcc acgcccaggg ctcccctgta ggagcgacgc
     5341 aagtcgcgat cgcagcgctc gaaacccgcg cgtgccgcta ttcccgcgca ccgccctgcg
     5401 cgagcacccg cccgaggaac tccaccagcc gcccgacgtg gtgctcgagg tcgacgaagg
     5461 attcgtggtc gcccgacaac gtcatcagct cgaccggcgt atcgccacgc atcgcgtaga
     5521 tctgcatggc ctcgtcgatc ggcaccacct cgtcgtccgc gccatgcacc agcagcaccg
     5581 ggcggcgaat gcgggcgatg gtgttcaccg gcgcgatgtc gtcgaagcgg tggccgatcg
     5641 tcttctgcac gtagtcgagg atgtagcgcc ccaacggctt ctcggggatg cgcttgcccg
     5701 ccagccagcg ccgcatcatc gccgccggat gcgagaacgc cgccacgctc accaccgccg
     5761 ccacctgcgg cgtgcgcgag gccgcgaaca gcacggcgcc ggcgccgacc gaatggccga
     5821 gcagcgcgat gcgcgccgga tccaccccgg gctgcgccgc cagccacgcg aaggcgtgct
     5881 cggcgtcctc ggcgaagcgc ggcagcgagg cgaagctgtc gtcgtcgctt gcgccgtggc
     5941 agcgcgcgtc gacgaacagc atcgcgtagc cggcctcgtg cagcgggcgg gcgagcggca
     6001 gcatcagcgc cgcattgccg ccccagccgt gcatcaccac cacggccggt gcggattcga
     6061 actcggcacg agcgtcatcg cgctcgccgt ccgcaaggtc cgcgcccggg cttgcagcgt
     6121 gatccgatcc atcacaggcg ctccccggcg ccggaatgaa ccacgcatgc agcgacttcc
     6181 catgctcggt gccgatgcgc agcgtctcga acgccagccc gagcgcgccc ggatcggtgt
     6241 gatgcggcac ccgcggcgcc gccaggccct tgcggatccc ccaatgcacc agcgcccgcc
     6301 cgccggcgag cgtggcggcg ccggcggtga aggctgcgat cagtccgggt gcgcccatgc
     6361 cgtttgctcc tcaggccgcc tgcagcagaa actcgacctt cacggcatcg aacggaaact
     6421 cgatccgccc atcttcagtg cgcaacagca cgccggcatc gagcagggcg gtgacgtcac
     6481 catgcaccgc cttgacgtcg cgcccgaccc ggcgcgcggc ctcgcggatc gacaccggac
     6541 ccgccgcaca caaggccttc agcaactccc agcgcttggc gctcaagact ttccagagca
     6601 gttcgggtgt cgcgaaggcg atccgcacct gagcgtcacc ccgcccggtt ttcattgccc
     6661 gcgcggcgtc cgccagcgtg tccgtcaggc tgcgcacttc aagaacgacc gtgttcatgg
     6721 ttccacctcg caatgtccgc tccgaagtca cgggtgagtt gatccggatc ggaaaagacg
     6781 tattccgctt ccgtgtcgcc gtaatgccgg tgatcgccct tgccgcgctc gttgtcgtag
     6841 cgcagaacac actcgccacc gaccacgtag gccaggcggt acttgaacgg atgcccggaa
     6901 ggcggcaccg gctccgaaac ccgccacacg gcgatctctg caaaagcatc cggcgcgagc
     6961 acgacccgac ggcggatcaa gggaatcgct ttcatgttgg acatattgac aacatctaag
     7021 aatgttgtca aagacaacaa cattcagctc tggagacccg cccaccacag ccgcgcgcgc
     7081 aggccccagc cggtggcgta gccgagcgac accgagcgga tcttgcccgc cgggtcgacg
     7141 atgacgaagg ccggcacgcc gcgcaggccg taggcctcgg caatgcggcc gtcggcgtcg
     7201 atggcggtgg tccaggccag gccgcgctcg gcgagcacgc ctgtcacctc cgccgcgccg
     7261 cccgactgca tggcgacggt gagcaccggg tggtccgcct gcacgccatc gatcgtgccc
     7321 tgctgtgcgg tgcagatcgg gcaccagtcg gcccagaaat acaccccgac cgccttgccc
     7381 ggatgcgcgg cgcgccattc ggcgaggctg agcccgcggc cgtcggcggc gacggtggcg
     7441 aaatccggcg cgaggccggc cgggacgtcg cgcgccatcc aggcctgcac cgcgaaaaag
     7501 atgacgacga agatgccgat ctccacgccc caacgccgcc agcgcggtgg cttgcggacg
     7561 ggctcggccg tttcgcactc ggcgttcgcg ggcgagcgtg cgccggcgcc ctccgcttcc
     7621 gcatgcggcg ccggcgcagg tcgtgaatct cccatgtcgt cggtcggctt cgtcatcgcg
     7681 gatcgatcca gcgcagcccg cccgcctcgc gctcgaacag gcgccagccg ccagcggcgt
     7741 tgcacaggcc gtagatgaag ggctgcgcat cgcgcccggg ggtgaggaag cgcaggtcct
     7801 tgaagctggc gcatgcctgg ccgtcgcccg cctcgccagc atggtcgagc accgggaaca
     7861 tcgcaaacca gcggtagaag gcgaagtcct ccgcgttcca cacctgctcg gcgagcgcgc
     7921 gcgtgctgcc ggtgccgaag cgctcgcgca cctcccactg cgccatcgcc accggcatat
     7981 agggcgcgga gaagcggcgg atgaagttgt cgtcctcggt ggccaccagc ggttcgctgc
     8041 gccgggtgtt gaggtgggcg tagtggtagc gctcgccgtc gaacacgatc gcggtccagt
     8101 tgaagggcga ggccgggcgc ggtgcggcat ccacctcgac tgcggcgatg cccttggccg
     8161 cggcgtaggc gcgcgcggcg tcgatcgcct cggcgcgccc cgtcgcctgc acgcccaccc
     8221 agccggcgag cagcaccagc gccgccgtcg ccggcacgcg gctgctgcgc catagcgcgc
     8281 tcgcgagcag gccggcgagg aggatgccgg tgaagggcag gtcgatgatg aagctcgtac
     8341 cccagccgaa gcggtggtcg gagaagggcg ccaggatcat ggtgccgaac tgggtgatga
     8401 ggtcgccgag gatgtgcacg aagatcgccg cgcacaccac cgggtagaag ctgcgccagc
     8461 ccgccgccac ccgtccgccg cgctgccaga agcgcgccag cagccaggcg atcagcacgc
     8521 cccacagcgg cagcaggatc agtgagtggg tgacgccgcg gtggccgcgc aggtaggtga
     8581 gctcggagat ccagccgagc acgaagtcga ggtcggggaa gatcgccgcc gcggtgccgg
     8641 cgaccaccat ctgccagacc ggcaccggcg ctgcgttcgc cgcccgggtg ccggcagcag
     8701 gccgacgcga ggccagcatg cggccgacga gggcgccgga gagggcgtgg gtgagggtat
     8761 ccatcgggta caggggttct ctgggttgcc ttgtccggct gccgtgcaaa ggcgggccgg
     8821 tcgcgcggct ggggcgcgtt cagtgggtgg agggccggct gtccatccgg ggggcgcccg
     8881 gccacagccg cgacagcgcc ggcagcaggc tgcgccaatc cggcactgac cgggtatcga
     8941 tcggcaggac gcgcgcgagt tcctcggcag cgaagccctc gcggctgcgc agttgcgcct
     9001 cgagcaccgc ctcgtcggcc tccgaggcgt cgccgccggc ggccaggcgg gcgcgcacgc
     9061 gcgcgcgcag gacgtcctcc ggcgcatcga aggcgaggat cgcgaagggc acgccgagct
     9121 cctgcgcgag cccggcgaag gccgcgcgct gggcgcgttt caggaaggtg gcgtcgacca
     9181 gcaccgggcg cccggcctcg agcaccacgc gcgccagcac ggccaggcgc gcataggtgc
     9241 gcgcggtggc ctccggggta tatagcccac cgccgaggcc cgagccgctc gtcgcctccg
     9301 ccgccaggcc ggcgaggcgc ttgcgctcga cgtcggcgcg cacgcggatc accccgcccg
     9361 cctccaccag ggcctgcgac tggctggtct tgcccgcgcc cgcgacgccg caggccacca
     9421 gcaaggccgg cgcgcccggc gccctctgcg cctcggcgag cgcaaggtag cgccggcatt
     9481 cgtccacgct ctcgctgcgt gcggcgccgt cgagctggct ggcgcggatc gcggcgatct
     9541 tggcgcgcac catcgcgcga tagctgaggt agtagggcag cacgcgcagg ccggcgtagt
     9601 cgccgcagcg ctccagccag gcattgagga agcgccagcc cagcgcggcc cgcccgcgcg
     9661 cctgcaggtc catgaccagg aaggcgacgt cggcgacgac gtcggtccag cgcagctcgg
     9721 cgctgaactc gatggcgtcg aagaggcgcg gctcgccgtc gagcacgacc aggttgccca
     9781 ggtgcaggtc gccatggcat tcgcgcaccc gcccctcggc caggcgcacg gcgaacaccg
     9841 gcgcgagcgc ggcgtactgc gcctcggccc aggcctccac ccgggccagc atcgcgagca
     9901 ggtcggcatg gacgccggga tcgaggcgct cgcgcatctg cgcgaagttc tgccgcaccg
     9961 gcgcatgcac cgcggccgca tcgccgaacg ccccgcccgg caacgccacc gcggcggcgg
    10021 cgtggaacgc ggcgacatgg cgggcgaggc gatcgaagag cgcgggcgcc agcctgccct
    10081 cgtcgagcag gcgcgagaac agcccggcct ggtcgaagcg gcgcatgcgc agcagccagt
    10141 cgaagggctc gccgccaccg tcgaggcggg gcgcctcggg gctgccgcgc accgccacca
    10201 ccgcctcgta gatctccggc accgtgcggc ggttgagccg cagctccgcc tcgcaggcct
    10261 cgcgccggcg cgccggcgtg gaaaagtcca ggaagccgag gtcgagcggc ttcttcagct
    10321 tccaggcgtg ctcgccggcg agcagcaccc aggagatgtg ggtctcgatc agctcgacgc
    10381 cgccctccga gccgggcagc acgccgggcc ggcgcagcgt gttcagcagg gtttcggatg
    10441 catgcatcgg cagctctcct cggtcatccg cccgatggta gcccgggacg cgcgcagccg
    10501 ggccgctcgc cgccgcgcca cggcctcccc tatcatcgcg tccgcctcca tcgacgaccg
    10561 ccgaccatgc ccgccactcc cttcaccgcc ctcgcctgcc cgctcgacgg cgcgccgctg
    10621 caacgccagg gcggcagcct gcgctgcgcg gacgggcact gcttcgacct cgccgcccag
    10681 ggctatgtga acctgctgcc ggtgcagaac aagcgctcgc gcgaccccgg cgacagcaag
    10741 gagatggtcg ccgcgcgccg gcgcttcctc gaggccgggc attacgcgcc gatcgccgcc
    10801 gcggtggcgc gcaccgtgtt cgagggcgcg gcccccggcc agacgctcgc ctgcctcgac
    10861 gccggctgcg gcgagggcta ctacctgcgc cagctctccg ccgcggcggt ggtggaagca
    10921 cgcacgctcg cgctcgccgg tctggacatc tcgaagtggg cggtgcaggc ggcggcgaag
    10981 cgcgatctgc aggacgcccg cagccgggcc ggcgccgatc cgtccaccgc ctggatcgtc
    11041 ggcagcaacg ccggcctgcc ggtgctgccg gccacgctcg accgcgtgct gtgcatgttc
    11101 ggcttcccgg tctaccccga gttcgcgcgc gtgctgcgcc ccggcggcga actgctgcag
    11161 gtcgacgccg gcccggacca cctgcgcgag ctgcgcgaag tcatctaccc cgcactcaag
    11221 cccgagcgcc cggccagccg cgcggcgccc ccgggcttcg agcacctcgc cagcgacacc
    11281 ctgcgcttcc agatccacgt cgacggcgca gcccagatcg ccgacctgct ggcgatgacg
    11341 ccccacctct accgcgccag cgccgaaggc cgcgcccgcg cggcggcgct cgtcgcgctg
    11401 acgatgacgg tggacgtgcg cctggcgcgc taccgccgcc agcccggctg aagccgaacc
    11461 gcgcaaggca atccgcacct gcagcgcccg ctcgacatga acggcggatg aacggcatct
    11521 tcagcatcga ttcagccagc gcggcgcagc atggcatcac ggtcgcaaca ggaccgccca
    11581 accgacacag gagatgccat catgcgtgcc accactctca tcgccaccct ggccctcacc
    11641 ggcggactca tcggcgccgg cgccgccatc gcccccgcgt tcgcccagaa cgcagccccc
    11701 gcagccaccc cggccgccgc gaccgccccc gccgcccaat ccaactggat gagcccgaag
    11761 gacgtgcaga ccaagctcga aggcgccggc tacaccgact tccgcgagat cgagcgcgac
    11821 aagaacaagt acgaggtcaa ggccaccgac ccgcagggcc agcgcgtgga gctggacgtc
    11881 gatccggtca ccggcgacgt cctcaagacc gaggtcaagc gcagcaagta agcgc
//
LOCUS       DAOQBS010000099        11869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4534_length_11869_cov_15.420857, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000099 DAOQBS010000000
VERSION     DAOQBS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11869
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4534_length_11869_cov_15.420857"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="PKN25_11165"
     CDS             <1..1550
                     /locus_tag="PKN25_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNV91502.1"
                     /translation="ANGILFGLVGIELGLLHGALFQALPERIFGIMANDTLLAVPFFT
                     FMGLILERSGMAEDLLDTIGQLFGPIRGGLAFAVIFVGALLAATTGVVAASVISMGLI
                     SLPIMMRYGYDHRLATGVIAASGTLAQIIPPSLVLIVMADQLGRSVGDMYQGALFPGL
                     VLTSLYVVYVAVVAIFRPSLAPAMPPEARTLREPDGASGLRSLALLTVVAGAGAWLFA
                     SQIYRADAPRDEVVVVSAMAGIGIAFVAAMVNRIFGIGLLSRMAERVTFVLIPPLGLI
                     FLVLGTIFIGVATPTEGGAMGALGAVLMALARRRLSLPLLRQAMDSTTKLSCFVVFIL
                     IGSSVFGLTFRGVNGDLWVEHLMTSLPGGQTGFLVAVNLLVFVLAFFLDYFELAFIIV
                     PLLAPVADKLGIDLIWFGVLLAVNMQTSFLHPPFGFALFFLRSVASEKVKTASIYWGA
                     VPFVIIQLVMVGLIIAFPGLVSYGDAGAKPAQQQELDLEQLLQGGGGAAAAGDSNADL
                     MRKLQGD"
     gene            complement(1615..2721)
                     /locus_tag="PKN25_11170"
     CDS             complement(1615..2721)
                     /locus_tag="PKN25_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNV91503.1"
                     /translation="MERRSFLKKASVGLAAGAAAGVASTAAVAAPAVQTGLPEIKWRM
                     TSSFPKSIDTLYGSAELLANRLREITGGKFDIRIFPAGEIVPGLQALDAVQQGTVEMC
                     HSCSYYYVGKDKTFAFGTAVPFGMNARQMDAWIIGGGGQELLDEFYGNYNVYSFLGGN
                     TGVQMGGWYRKQINTLEDIKGLKIRIAGIAGEIMSRMGAIPQQIAGSDIYPSLEKGTI
                     DAAEWVAPYDDEKLGFYKVAPHYYSPGWWEPGPVVHFYVNKAEWDKLPKEYQAAFRAA
                     SREAHIQMTAMYDHKNPQALARLLAQGVKLQNFSNEIMKKAFDISRELYAEEAAKNPA
                     WAKIYAEFEKYRKSQNAWFSVAEAGFDRFMQSVR"
     gene            complement(2838..4184)
                     /gene="pmbA"
                     /locus_tag="PKN25_11175"
     CDS             complement(2838..4184)
                     /gene="pmbA"
                     /locus_tag="PKN25_11175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HNV91504.1"
                     /translation="MSSQGFSFSQAHLAEIATDILKYARKHGASSCETDVSEGFGQSV
                     AVRRGEVDTIEYNRDKGVGVTVYLGQQRGYASTSDFSKKALKATVDAALSIARFTAPD
                     PCAGLADAALMAKAKDMPELDLFHPWNIDVAQAIELARRCEDAAFAVSPKIGNSEGAN
                     TSIQQSHFVSANSHGFLGGYATTRHGLSCSVIAGEGDAMQREYWYDSRRDPAELMVAE
                     EVGRRAGERALARLGAKKIRTCEVPVLFEAQLAVALVGNFVQAVSGGSLYRKSSFLLD
                     SLGKQVFSPVVSIAERPHLKKAFGSSPFDGDGVATRDREVVTDGVLNGYFLSAYSARK
                     LGLQTTANAGGSHNLIVKPGEQDLAGLIRQMDRGLLVTELLGHGVNYVTGDYSRGAAG
                     FWVEKGRIKHAVEEITIAGNLRDMFRSIVAVGNDALPRGAKLCGSVLIERMKVAGR"
     gene            4250..4834
                     /gene="yjgA"
                     /locus_tag="PKN25_11180"
     CDS             4250..4834
                     /gene="yjgA"
                     /locus_tag="PKN25_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HNV91505.1"
                     /translation="MRHHHSDPADEDDFPEPPSKSSRKREMQALQDLGEQLVALSPER
                     LKKVPLPETLYEAIRAAQGFKMEARRRQLQYIGKLMRKIDPEPIQAQLDIFAGNSAAE
                     VAKMHRLERLREQLLEDEQTIGTIAETWPEADLQYLRTLRRNALKEREAAKPPKSFRE
                     IFRVLRELQEARDAAAEAAETPSDEDAAEQGPNP"
     gene            4831..5421
                     /gene="mog"
                     /locus_tag="PKN25_11185"
     CDS             4831..5421
                     /gene="mog"
                     /locus_tag="PKN25_11185"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HNV91506.1"
                     /translation="MSEHIRIGLVSISDRASEGTYEDQGIPALKDWLGRALTSPWSAE
                     TRLIPDERALIERTLVELVDRAGCNLVLTTGGTGPAVRDVTPEATLAIGDKEMPGFGE
                     EMRRISLNFVPTAILSRQVAVIRGKCLIINLPGQPKSIRETLEGVRDADGSVRHVGIF
                     AAVPYCIDLVGGPYVETDDAMIKAFRPKHAMRPKQA"
     gene            5445..6695
                     /locus_tag="PKN25_11190"
     CDS             5445..6695
                     /locus_tag="PKN25_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV91507.1"
                     /translation="MTWEELADDGSVRRCSARWRSESGQAPPRRVVIADDTTKADAAW
                     RLACEGSALLWRGDYHNARQLLQAMARRADARARKPGRAVKPGKAPATPAEAFHLHRM
                     AQAQRARALGMLLIPFEAGVRIPLRRAPEVAEVVAEAVAGTADADLAAAPFVMSLREL
                     LGLVGAHEWRRKGVAIPALGERIHPWYGVYSPIRGEYLDLIARAPLPATTLAFDIGTG
                     TGVIAALLARRGVARVVATDLSPRALGCAQQNLARLELLAGAAAEEGAPGTTSCTIPG
                     AAAPDLQVAAGRVQLLQADLFPPGRAPLVVCNPPWVPAKPSSAIEHAVYDPDSRMLRG
                     FLAGLAEHVEEGGEGWLILSDLAEHLGLRSRAELLGWIEAAGLRVLGREDIRPRHGKA
                     ADADDPLHAARAAETTSLWRLAPR"
     gene            complement(6735..7418)
                     /locus_tag="PKN25_11195"
     CDS             complement(6735..7418)
                     /locus_tag="PKN25_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNV91508.1"
                     /translation="MNLSLRSPSAGTIVAFKAIVFALCLLPAVNLALGWQANALGANP
                     IETITRASGEWTLRLLLITLAVTPLRRFSGLHWLLRLRRMLGLFAFAYALGHFAIYLW
                     LDQFFDWNAIALDILDRPFITVGFAAFVLLIPLAATSNSFAIRRLGGRRWQSLHRSVY
                     AIAIFAVVHFWWLVKADIGRPLLYASILAVLLGLRGWWRELERRRQLSVPPPAKHLEK
                     KVIRLVPRQ"
     gene            complement(7444..8379)
                     /gene="msrP"
                     /locus_tag="PKN25_11200"
     CDS             complement(7444..8379)
                     /gene="msrP"
                     /locus_tag="PKN25_11200"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004378077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNV91509.1"
                     /translation="MLIHKPADVPPSEITPRSLFERRRNFLARSGTGLVAGAALWHGL
                     PGAARASTRLQTVPSALSTTEPLSERDAVTGYNNFYEFGTDKADPARNAHKMEVRPWT
                     VRVEGLVGKPRRFDIDELLRLAPLEERIYRLRCVEGWSMVVPWVGFPLAALLKEVEPL
                     GSAKYVEFVTHYDPKIMIRDVLDWPYTEGLRLDEALHPLTLLAVGVYGEALPKQNGAP
                     LRLVVPWKYGFKSAKSIVTIRLTEKEPATAWNKFAPQEYGFYSNVNPEVRHPRWSQAT
                     ERRIGELRKRPTQMFNGYAEQVASLYQGMDLKKFY"
     gene            8560..9642
                     /gene="aroG"
                     /locus_tag="PKN25_11205"
     CDS             8560..9642
                     /gene="aroG"
                     /locus_tag="PKN25_11205"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HNV91510.1"
                     /translation="MSASIKLHIDDVRIQEIKELVPPAHVLREYPATPKAAEVAYEAR
                     QAIHRILYGADDRLLVVIGPCSIHDAKAALEYAHKLKAEAERVKDDLLIVMRVYFEKP
                     RTTVGWKGLINDPGLDNSFRINDGVRLARKLLWEVNEIGLPAGTEFLDMITPQYIADL
                     IAWGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLRIAADAIKAAQAPHHFLSVTK
                     AGHSAIVSTRGNEDCHAILRGGKEPNFDADSVEAASRELAAAGVCNKVMVDFSHGNSR
                     KQHRLQIEVAKNVAAQLAAGDDRIMGVMVESHLKEGRQDLVPGKELEYGKSVTDACIG
                     WEDSIDVLDILAEGVRQRRVKRAAEF"
     gene            9728..10837
                     /gene="dinB"
                     /locus_tag="PKN25_11210"
     CDS             9728..10837
                     /gene="dinB"
                     /locus_tag="PKN25_11210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNV91511.1"
                     /translation="MRKIIHCDCDCFYASVEMRDDPTLRGRPVAVGGRAETRGVIATC
                     NYEARAFGVHSAMPTARALQLCPQLVLLAPDFERYREASRRILAIYHDYTALVEPLSL
                     DEAYLDVTGVERCRGSASLMAQEIRARIRAEVGITASAGIAPNKFIAKVASDWNKPDG
                     QFVVRPQDVDAFVAALPVKKIFGVGKVTAARLARLGAHTCADLRAWSVAELTREFGSF
                     GASLHRLCRGIDERPVQPDRVRKSLSVETTYTPDLHDLAACRAGLLALLEDFLRRFER
                     ARDRPPVHKAFVKVRFSDFSRTTAECIASAPDQATWLRLLDEAHARKALPVRLLGVGV
                     RFAEDEGGERPAQIPLFDPESDPNPDPARAAAEPH"
     gene            10851..>11869
                     /locus_tag="PKN25_11215"
     CDS             10851..>11869
                     /locus_tag="PKN25_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNV91512.1"
                     /translation="MPDLETSAAAAEPPPAPQPPAVAGEQPVGIGALYRALWEHAAGA
                     RQLLLYAFLLLLASQLFKLAVPWLAGNAINHIQSGGLEGMSEAGRWLGMVFAAIVASW
                     LLHGPGRILERNVALKVRERLSALLVGRLLAAPLAWHERHHSGETTHRMQQSTHALYD
                     FAQSQFIYLQNTVRLVGPIVALWLISPLVGAVALVGYGLIGLLITRFDRVMMRVAVEE
                     NRAERAYSATLVDVLGNVLSVLALRRGEGVARLIGERLAAVFGPLKKSIVLNEAKWCA
                     VDLLASALWCVLVGVYAAEAAGVFGFGAAAGGAAAAAGTIALGKVFMVYEYSQQAGGV
                     ITAIAA"
BASE COUNT         1745 a         4149 c         4128 g         1847 t
ORIGIN      
        1 cggccaacgg catcctgttc ggcctcgtcg gcatcgagct cggcctgctc cacggtgcgc
       61 tgttccaggc gctgccggag cgcatcttcg gcatcatggc caacgacacc ctgctcgcgg
      121 tgcctttctt caccttcatg gggctgatcc tcgagcgcag cggcatggcc gaggatctgc
      181 tcgacaccat cggtcagttg ttcgggccga tccgcggcgg cctcgccttc gcggtgattt
      241 tcgtcggcgc gctgctcgcg gcgaccaccg gcgtggtcgc ggcctcggtg atctcgatgg
      301 gtctgatctc gctgccgatc atgatgcgct acggctacga ccatcgcctc gccaccggcg
      361 tgatcgccgc ctccgggacg ctggcgcaga tcatcccgcc ctcgctggtg ctgatcgtga
      421 tggccgacca actcggccgc agcgtcggcg acatgtatca aggcgcgctg ttcccgggct
      481 tggtcctcac cagcctgtat gtggtctatg tcgcggtggt ggcgatcttc cggccgtcgc
      541 tcgcgccggc gatgccgccc gaggcgcgga cgctgcgcga gcccgatggc gccagcgggt
      601 tgcgctcgct ggccctgctg accgtggtcg cgggcgccgg cgcctggctc ttcgccagcc
      661 agatctatcg cgccgatgca ccgcgcgacg aggtggtggt ggtgtcggcg atggccggga
      721 tcggcatcgc gttcgtggca gcgatggtca accgcatctt cggcatcggc ctgctgtcgc
      781 gcatggccga gcgcgtgacc ttcgtgctga tcccgccgct cgggctgatc ttcctcgtcc
      841 tcggcaccat cttcatcggc gtcgcgacgc cgaccgaggg cggtgcgatg ggggccctcg
      901 gcgcggtgct gatggcgctc gcgcgccggc gcctgtcgct gccgctgctc aggcaggcga
      961 tggattcgac caccaagctg tcgtgcttcg tggtcttcat cctgatcggc tccagcgtgt
     1021 tcggcctcac cttccgcggt gtcaacggcg acctctgggt cgagcacctg atgacctcgc
     1081 tgcccggcgg gcagaccggc ttcctggtcg cggtgaacct gctcgtcttc gtgctcgcct
     1141 tcttcctcga ctacttcgag ctcgccttca tcatcgtgcc cctgctcgcg ccggtggcgg
     1201 acaagctcgg catcgacctg atctggttcg gcgtgctgct ggcggtgaac atgcagacct
     1261 ccttcctgca tccgcccttc ggcttcgcgc tcttcttcct gcgctcggtg gcgtcggaga
     1321 aggtgaagac ggcgtcgatc tactggggtg cggtgccctt cgtgatcatc cagctcgtca
     1381 tggtcggcct catcatcgcc ttccccggcc tggtgtccta cggcgacgcg ggcgccaagc
     1441 cggcgcagca gcaggaactc gacctcgagc aactgctgca gggcggcggc ggggccgcag
     1501 cggcgggcga ttcgaacgcc gacctgatgc ggaaactgca gggcgactga gtgcgcgcac
     1561 catgaaaaaa gccgggcatg cccggctttt tttgcgcctg aagcctgaag cccgtcagcg
     1621 caccgactgc atgaagcggt cgaagcccgc ctcggcgacg ctgaaccacg cgttctgcga
     1681 cttgcggtac ttctcgaact cggcgtagat cttggcccac gccgggttct tcgcagcctc
     1741 ctcggcgtac agctcgcgcg agatgtcgaa ggccttcttc atgatctcgt tggagaagtt
     1801 ctgcagcttg acgccctgcg cgagcaggcg ggcgagcgcc tgcgggttct tgtggtcgta
     1861 catcgcggtc atctggatgt gcgcctcgcg cgaggcggcg cggaaggcgg cctggtattc
     1921 cttgggcagc ttgtcccact cggccttgtt cacatagaag tgcaccaccg ggcccggctc
     1981 ccaccagccc ggcgagtagt agtggggcgc gaccttgtag aagccgagct tctcgtcgtc
     2041 gtagggggcg acccactcgg cggcgtcgat ggtgcccttc tcgagcgagg ggtagatgtc
     2101 cgagccggcg atctgctgcg ggatcgcgcc catgcgcgac atgatctcgc cggcgatgcc
     2161 ggcgatgcgg atcttcaggc ccttgatgtc ctccagggtg ttgatctgct tgcggtacca
     2221 gccgcccatc tgcacgccgg tgttgccgcc gaggaaggaa tacacgttgt aattgccgta
     2281 gaactcgtcg agcagctcct ggccgccgcc gccgatgatc caggcgtcca tctggcgcgc
     2341 gttcatgccg aagggcacgg cggtgccgaa ggcgaaggtc ttgtccttgc cgacgtagta
     2401 gtaggagcac gagtggcaca tctcgaccgt gccctgctgc accgcgtcga gcgcctgcag
     2461 gccggggacg atctcgccgg ccgggaagat gcggatgtcg aacttgccgc cggtgatctc
     2521 gcgcaggcgg ttggcgagca gttcggcgct gccgtacagg gtgtcgatgc tcttggggaa
     2581 gctcgaggtc atgcgccact tgatctcggg caggccggtc tggacggcgg gggcggcgac
     2641 ggccgcggtg gaggcgacgc ctgcggcggc accggcggca aggccgacgc tggccttctt
     2701 gaggaatgaa cgacgttcca cgatttctcc ttgtgttcgg gggtggattc cagcgaattc
     2761 agcgaacgat tatagacgcc gctcgcgcaa cgggacggaa aggtaccgcg ctggcgggat
     2821 gaagtccgga caccggctca gcgcccggcg accttcatgc gctcgatcag caccgagccg
     2881 cacagcttgg cgccgcgcgg cagggcgtcg ttacccaccg cgacgatgct gcggaacatg
     2941 tcgcgcaggt tgccggcgat ggtgatctcc tcgacagcgt gcttgatgcg gcccttctcc
     3001 acccagaagc ctgccgcacc gcgcgagtaa tcgcccgtga cgtagttcac gccgtggccg
     3061 agcagctcgg tcacgagcag gccgcggtcc atctgccgga tcagtccggc gaggtcctgc
     3121 tcgccgggct tcacgatcag gttgtgcgag ccgccggcgt tggcggtggt ctgcaggccg
     3181 agcttgcgcg ccgaataggc ggagaggaag tagccgttca gcaccccgtc ggtgaccacc
     3241 tcgcggtcgc gggtggccac gccgtcgccg tcgaaggggc tggagccgaa ggccttcttc
     3301 aggtgcgggc gctcggcgat gctcaccacc ggcgagaaca cctgcttgcc gaggctgtcg
     3361 agcaggaagc tcgacttgcg gtagagcgag ccgccgctca ccgcctgcac gaagttgccg
     3421 acgagcgcca ccgcgagctg cgcctcgaac agcaccggca cctcgcaggt gcggatcttc
     3481 ttcgcaccca ggcgcgccag cgcgcgctcg ccggcgcgcc ggccaacctc ctcggccacc
     3541 atcagctcgg cagggtcgcg ccgcgagtcg taccagtatt cgcgctgcat ggcgtcgccc
     3601 tcgccggcga tcaccgagca cgacagaccg tggcgggtgg tggcgtagcc gccgaggaag
     3661 ccgtggctgt tcgccgacac gaagtgcgac tgctggatcg aggtgttggc gccctccgag
     3721 ttgccgatct tcgggctgac cgcgaaggcg gcgtcctcgc agcggcgggc gagttcgatg
     3781 gcctgcgcga cgtcgatgtt ccacggatgg aagaggtcga gctcgggcat gtccttcgcc
     3841 ttcgccatca gcgccgcgtc ggccagcccg gcgcaggggt cgggcgcggt gaagcgcgcg
     3901 atcgagagcg cggcgtccac cgtggccttg agcgccttct tcgagaagtc ggaggtgctc
     3961 gcgtagccgc gctgctggcc gaggtacacc gtgacgccca cccccttgtc gcggttgtac
     4021 tcgatggtgt cgacctcgcc gcggcgcacc gcgaccgact ggccgaagcc ctcggagacg
     4081 tcggtctcgc acgacgaggc gccgtgcttg cgggcgtatt tcaggatgtc ggtggcgatc
     4141 tcggcgagat gcgcctgcga gaaggagaag ccttggctgg acatgggcgg gccgggtgcg
     4201 tgcaaaggct ataatcttac cccgatcgag ccagcccccc atgcaagcaa tgcgccacca
     4261 ccattccgac cccgcggacg aggacgattt tcccgagccg ccgagcaaga gcagccgcaa
     4321 gcgcgagatg caggcgctgc aggacctcgg cgagcagctc gtcgcgctct cgcccgagcg
     4381 cctcaagaag gtgccgctgc ccgagaccct gtacgaggcc atccgcgccg cccagggttt
     4441 caagatggag gcgcgccgcc gccagctgca gtacatcggc aagctgatgc gcaagatcga
     4501 ccccgagccc attcaggccc aactcgacat cttcgccggc aactccgccg ccgaggtggc
     4561 gaagatgcac cgcctcgagc gcctgcgcga gcagctgctc gaagacgagc agaccatcgg
     4621 caccatcgcc gagacctggc ccgaggccga cctgcaatac ctgcgcaccc tgcgccgcaa
     4681 cgcgctcaag gagcgcgagg ccgccaagcc gccgaagtcc ttccgcgaga tcttccgcgt
     4741 gctgcgcgag ctgcaggaag cccgcgacgc cgccgccgag gcggcggaga ccccgtccga
     4801 cgaggacgcc gcagaacagg gaccgaaccc atgagcgaac acatccgcat cggcctcgtg
     4861 tcgatcagcg accgcgcctc cgagggcacc tacgaagacc agggcatccc cgcgctgaaa
     4921 gactggctgg gcagggcgct cacgtcgccg tggagcgccg agacccggct gatccccgac
     4981 gagcgtgcgt tgatcgagcg caccctggtg gagctggtcg accgggccgg ctgcaacctc
     5041 gtgctcacca cgggcggcac cggtccggcg gtgcgcgacg tgacccccga ggccacgctc
     5101 gcgatcggcg acaaggagat gcccggcttc ggcgaggaga tgcgccgcat cagcctgaac
     5161 ttcgtaccca ccgcgatcct gtcgcgccag gtcgcggtga tccgcggcaa gtgcctcatc
     5221 atcaacctgc ccggccagcc caagtcgatc cgcgaaaccc tcgaaggcgt gcgcgacgcc
     5281 gacggcagcg tgcgccacgt cggcatcttc gccgcagtgc cgtactgcat cgacctcgtc
     5341 ggcggtccgt atgtcgagac cgacgacgcg atgatcaagg ccttccgccc caagcacgcg
     5401 atgcggccga agcaggcgtg agcacctcga gcgcagcggc gacgctgacc tgggaagagc
     5461 ttgcggacga cggcagcgtg cgccgctgca gtgcgcgctg gcgttccgag agcggccagg
     5521 cgccaccgcg ccgggtggtg atcgccgacg acaccaccaa ggccgatgcg gcctggcgcc
     5581 tggcctgcga gggcagcgcc ctgctgtggc gcggcgacta ccacaacgcc cgccagctgc
     5641 tgcaggcgat ggcgcgccgc gccgacgcgc gcgcgcggaa accgggcagg gcggtcaagc
     5701 cgggcaaggc gccggccacg cccgccgagg ccttccacct ccaccgcatg gcgcaggcgc
     5761 agcgcgcgcg cgccctgggc atgctgctga tccccttcga ggccggcgtg cgcattccgc
     5821 tgcggcgcgc gcccgaggtc gccgaagtcg tcgccgaggc ggtcgccggc acagcggatg
     5881 cggacctcgc tgcggcaccc ttcgtgatgt cgctgcgcga gctgctcggc ctcgtcggcg
     5941 cgcacgagtg gcggcgcaag ggcgtcgcca tccctgcgct cggcgagcgc atccacccct
     6001 ggtacggcgt gtattcgccg atccgcggcg aatacctgga cctgatcgcg cgcgcgccgc
     6061 tgcccgcgac cacgctcgcc ttcgacatcg gtaccggcac cggcgtcatc gccgcgctgc
     6121 tcgcgcgccg cggggtggcg cgcgtggtgg ccaccgacct gtcgccgcgt gcgctgggtt
     6181 gcgcgcagca gaacctcgcg cgcctcgagt tgctggccgg ggccgcggcg gaggagggcg
     6241 ccccgggcac aacctcctgc acgatcccgg gcgccgctgc gcccgacctt caggttgccg
     6301 ccggccgcgt gcagttgctc caggccgatc tcttcccgcc cggccgcgcg ccgctggtgg
     6361 tgtgcaaccc gccctgggtg ccggccaagc cgagctcggc gatcgagcac gcggtctacg
     6421 accccgacag ccgcatgctg cgcggcttcc tcgccggcct ggcggaacac gtggaggaag
     6481 gtggcgaggg ctggctgatc ctgtcggacc tcgccgaaca cctcggcctg cgcagtcgcg
     6541 ccgagcttct cggctggatc gaggccgcgg gactgcgcgt cctcggacgc gaggacatcc
     6601 gtccgcgcca cggcaaggcg gccgatgcgg acgatccgct gcatgcggcg cgcgcggcgg
     6661 aaacgacctc gctctggcgc ctggctccgc gctgaggcgt ggcgccgccg ggcccgacgg
     6721 cgccgcggtg ccgcctactg ccgcggcacc agccggatca ccttcttctc caggtgcttc
     6781 gccggcggcg gcaccgagag ctgccgccgg cgttcgagtt cgcgccacca gccgcgcagg
     6841 ccgagcagca cggcgaggat gctcgcgtag agcagcggac gcccgatgtc cgccttcacc
     6901 agccaccaga agtgcaccac ggcgaagatc gcgatcgcat agacagagcg gtgcagcgac
     6961 tgccagcggc gcccgccgag gcgtcggatc gcgaagctgt tcgaggtcgc ggcgagcgga
     7021 atgagcagca cgaaggcggc gaagccgacg gtgatgaagg ggcggtcgag gatgtcgagg
     7081 gcgatcgcgt tccagtcgaa gaactggtcc agccacagat agatcgcgaa gtgcccgagc
     7141 gcgtacgcga acgcgaagag gccgagcatg cggcgcaggc gcagcagcca gtgcaggccg
     7201 ctgaagcggc gcagcggcgt tacggcgagt gtgatcagca gcaggcgcag cgtccattcc
     7261 ccgctcgcgc gcgtgatcgt ctcgatcggg ttggcgccga gcgcgttcgc ctgccagccg
     7321 agcgcaaggt tcactgcggg cagcaggcag agggcgaaca cgatcgcctt gaaggcgacg
     7381 atcgtgcccg cggaggggct tctgagggaa agattcatcg ttgcggtcgg cgggatgcag
     7441 tgctcagtag aacttcttca ggtccatgcc ctggtacagg ctcgcgacct gctccgcata
     7501 gccgttgaac atctgcgtcg ggcgcttgcg cagctcgccg atgcggcgct cggtagcctg
     7561 gctccagcgc ggatggcgca cctcggggtt cacgttggag tagaacccgt actcctgcgg
     7621 cgcgaacttg ttccacgcgg tcgccggctc cttctcggtc aggcggatgg tgacgatcga
     7681 cttggcgctc ttgaagccgt atttccacgg caccaccagg cgcagcggcg cgccgttctg
     7741 cttgggcagc gcctcgccgt acacgcctac cgcgagcagg gtcagcgggt gcaaggcctc
     7801 gtccaggcgc aggccctcgg tgtagggcca gtcgagcacg tcgcggatca tgatcttcgg
     7861 atcgtagtgg gtgacgaact cgacgtactt ggcgctgccc aggggctcga cctccttcag
     7921 cagcgcggcg agcgggaagc ccacccaggg caccaccatg gaccagcctt cgacgcagcg
     7981 caggcggtag atgcgttctt ccagcggtgc caggcgcagc agctcgtcga tgtcgaagcg
     8041 gcgcggcttg ccgaccaggc cctcgacacg caccgtccac gggcgcacct ccatcttgtg
     8101 ggcgttgcgc gccggatcgg ccttgtcggt gccgaactcg tagaagttgt tgtagccggt
     8161 gaccgcgtcg cgctcgctga gcggttcggt cgtgctcagt gccgagggca cggtctgcag
     8221 ccgggtcgat gcgcgcgcgg cgccgggcag gccgtgccac agcgccgcgc ccgccaccag
     8281 cccggtgccg ctgcgcgcga ggaagttgcg acggcgctcg aacaggctgc gcggggtgat
     8341 ttcggagggc gggacgtcgg cgggcttgtg gatcagcatg attgcggact cgtctggaac
     8401 ggaggattcg gtggtgcgcg gcgggcggca gtgtacgaac agacgcgcgc cgcgccgatc
     8461 ggttcatccc atccggccta tagtctgcat tgcgtccagc catgctcgcc tttccgctag
     8521 aatcaggcat cccagcaaag atccgagaaa gagcctcgca tgtccgcctc catcaaactc
     8581 cacatcgacg acgtccgcat ccaggagatc aaggagctcg tgccgcccgc gcacgtgctg
     8641 cgtgaatacc ctgccacgcc gaaggcggcc gaggttgcct acgaggcgcg ccaggcgatc
     8701 catcgcatcc tgtacggcgc cgacgatcgt ctgctggtgg tgatcggccc gtgctcgatc
     8761 cacgatgcca aggcggcgct ggagtatgcg cacaagctca aggccgaggc cgagcgcgtc
     8821 aaggacgacc tgctgatcgt gatgcgggtg tatttcgaga agccgcgcac caccgtgggc
     8881 tggaagggcc tgatcaacga tcccgggctg gacaacagct tccgcatcaa cgacggcgtg
     8941 cgtctggcac gcaagctgct gtgggaggtg aacgagatcg gcctgcccgc cggcaccgag
     9001 ttcctcgaca tgatcacccc gcagtacatc gccgacctga tcgcctgggg cgcgatcggc
     9061 gcgcgcacca ccgagagcca ggtgcaccgc gagctcgcct ccggcctgtc ctgcccggta
     9121 ggcttcaaga acggcaccga cggcaacctg cgcatcgccg ccgacgcgat caaggccgcg
     9181 caggcgccgc accacttcct gtcggtgacc aaggccgggc actcggcgat cgtgtcgacc
     9241 cgcggcaacg aggattgcca cgccatcctg cgcggcggca aggagcccaa cttcgacgcc
     9301 gacagcgtcg aggccgccag ccgcgagctc gccgcggccg gagtgtgcaa caaggtcatg
     9361 gtcgacttct cccacggcaa cagccgcaag cagcaccgcc tgcagatcga ggtggcgaag
     9421 aacgtcgccg cccagcttgc cgccggtgac gaccgcatca tgggcgtgat ggtcgagtcg
     9481 cacctcaagg aaggccgcca ggacctcgtc ccgggcaagg agctcgagta cggcaagagc
     9541 gtcaccgacg cctgcatcgg ctgggaagac agcatcgacg tgctcgacat cctcgccgag
     9601 ggtgtgcgcc agcgccgcgt caagcgcgcc gccgagttct gagcgcgggc gccgttcgct
     9661 cggcctgccc ccggcgggtc cgtccgcccc tggcgcctca ctcccgactc ctcacgcgcg
     9721 cgggcgcatg cgcaagatca tccactgcga ttgcgactgc ttctacgcgt cggtcgagat
     9781 gcgcgacgac cccacgctgc gcggcaggcc ggtcgcggtg ggcgggcgcg ccgagacgcg
     9841 cggcgtcatc gcaacctgca actacgaggc gcgcgccttc ggcgtgcatt cggcgatgcc
     9901 caccgcgcgt gcgctgcagc tgtgcccgca gctcgtcctg ctggcgcccg acttcgagcg
     9961 ctaccgcgag gcctcgcgcc gcatcctggc gatctaccac gactacacgg cgctggtgga
    10021 gccgctctcg ctcgacgagg cctacctcga cgtgaccggg gtcgagcgct gccgcggctc
    10081 ggcctcgctg atggcgcagg agatccgcgc gcgcatccgc gccgaggtcg gcatcaccgc
    10141 gtcggcgggc atcgccccca acaagttcat cgccaaggtg gcgagcgact ggaacaagcc
    10201 cgacggccag ttcgtggtgc ggccgcagga cgtcgacgcc ttcgtcgccg cgttgccggt
    10261 gaagaagatc ttcggcgtcg gcaaggtcac cgcggccagg ctcgcccgcc tgggcgcgca
    10321 cacctgcgcc gacctgcgcg cctggagcgt ggcggagctc acacgcgagt tcggcagctt
    10381 cggcgccagc ctgcaccgcc tgtgccgcgg catcgacgag cgcccggtgc agcccgaccg
    10441 cgtgcgcaag tcgctttcgg tcgagaccac ctacacgccc gacctgcacg acctcgccgc
    10501 ctgccgagcg ggcttgctgg cgctgctgga agacttcctc cgccgcttcg agcgcgcgcg
    10561 cgatcgcccg ccggtgcaca aggccttcgt caaggtgcgc ttttccgact tcagccgcac
    10621 caccgccgag tgcatcgcca gcgcgcccga ccaggccacc tggctgcgcc tgctcgacga
    10681 ggcgcatgcg cgcaaggcgc tgccggtgcg cctgctcggc gtcggcgtgc gcttcgccga
    10741 ggacgagggc ggcgagcggc cggcgcagat cccgctattc gatcccgaat ccgatcccaa
    10801 ccccgatccc gcgcgcgcag cggcggaacc ccactgacgc ggagcctgcg atgcccgacc
    10861 tcgaaaccag cgcggccgcg gccgaacccc cgcccgcgcc gcagccgcct gcggtcgccg
    10921 gcgagcagcc ggtgggcatc ggcgcgctct accgcgcgct gtgggagcac gccgcaggcg
    10981 cgcgccagtt gcttctctac gccttcctgc tgctgctcgc gtctcagctc ttcaagctcg
    11041 ccgtgccctg gctggcgggc aacgcgatca accacatcca gtccggcggg ctggagggca
    11101 tgagcgaggc cgggcgctgg ctcggcatgg tgttcgcggc catcgtcgcg agctggctgc
    11161 tgcacgggcc ggggcgcatt ctcgaacgca acgtggcgct caaggtgcgt gagcgcctgt
    11221 cggcgctgct cgtggggcgc ctgctcgccg cgccgctggc ctggcacgag cgccaccact
    11281 ccggcgagac cacccaccgc atgcagcaga gcacgcacgc gctctacgac ttcgcgcaga
    11341 gccagttcat ctacctgcag aacaccgtgc gcctggtggg gccgatcgtg gcgctgtggc
    11401 tgatctcgcc gctggtgggt gcggtggccc tggtcggcta cggcctcatc ggcctgctca
    11461 tcacccgctt cgaccgcgtg atgatgcgcg tggcggtgga ggagaaccgc gccgagcgcg
    11521 cctactccgc gaccctggtc gatgtgctcg gcaacgtgct gtcggtgctc gcgctgcgcc
    11581 gcggcgaggg cgtggcgcgg ctgatcggcg agcgcctggc ggcggtgttc gggccgctga
    11641 agaagagcat cgtgctcaac gaggccaagt ggtgcgcggt ggatctgctc gcgagcgcgc
    11701 tgtggtgcgt gctggtcgga gtgtatgcgg ccgaggccgc gggtgtgttc ggcttcggcg
    11761 cggccgcggg cggggctgcg gcggcggccg gcacgatcgc gctcggcaag gtcttcatgg
    11821 tgtacgagta ctcgcagcag gccggcgggg tgatcaccgc gatcgcggc
//
LOCUS       DAOQBS010000100        11818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4569_length_11818_cov_16.101505, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000100 DAOQBS010000000
VERSION     DAOQBS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11818
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4569_length_11818_cov_16.101505"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PKN25_11220"
     CDS             <1..620
                     /locus_tag="PKN25_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91513.1"
                     /translation="RPHQSASMQKERWNHQTPETGSFAMAGFDIRNDTIWPLEISLGM
                     LSPLYWGLTEPAQTFSRSTGAVWFTIQATVALDHKEHITTAGAVFDVFKNILFPQVML
                     GNWLINGEMLAGSHGITEDVAQALGAKRPITIKGRPLDEMSTEDAIAGLQGIFTHDRS
                     RVSKAGCYAGWSGHKQYVVSGGPTLQVRAGGVELVSGTPLSIVKL"
     gene            complement(736..1398)
                     /locus_tag="PKN25_11225"
     CDS             complement(736..1398)
                     /locus_tag="PKN25_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HNV91514.1"
                     /translation="MSHHSGLRRRLVLGGSATLLSAPLAAAQRVLTPSQMPGPFYPVT
                     PPLHKDNDLTRVEGRSGRAAGQIADLSGRVLDLDGRPLAGLRVEIWQCDANGRYRHPR
                     DRADVEEDPDFQGFGHTLTDADGRYRFRTIRPVPYPGRTPHIHAAVFQEGGRPFVTQI
                     YVAGEPLNQRDGLFMRVPEALRPLLLADFVAADDPVATLAAEFDFVLAPALAGLFEAS
                     TA"
     gene            1401..2690
                     /locus_tag="PKN25_11230"
     CDS             1401..2690
                     /locus_tag="PKN25_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HNV91515.1"
                     /translation="MRLRLWTCREDRRGGARFRACPDARVGLGGRWGRRRRRRSRACP
                     QARPDCLGDWVVGRRRGMERRRFIGAAALAGLGVVGGAGVLSHRQSVAVYEAAAGTTW
                     RHGEPAQRDTRAALRELVRYATLAPSSHNTQCWKFALAERSVSILPDYSRRCPVVDPD
                     DHHLFVSLGCAAENIVQAAPAMGFHCEAAFDGEAAEVLNCALAPAPSRRTALFEAIPQ
                     RQSTRGEFDSRPLSNEELRRLEAAGSGRGVSVRLLTGAQALERVLEFVVAGNTAQMRD
                     PAFVRELKAWIRFNGSDAARTGDGLFAGASGNPAAPAWLGGLLFDAFFTEAAENDKYA
                     RQIRSAAGIAVFVSERDDRAHWIEAGRCFQRFALQATAIGVRTAHLNQPVELATLRPA
                     FAADLGITNGRPDLVIRFGRGPAMPRSLRRPLDAVLL"
     gene            complement(2716..4017)
                     /locus_tag="PKN25_11235"
     CDS             complement(2716..4017)
                     /locus_tag="PKN25_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HNV91516.1"
                     /translation="MSSLFEQHPLRQTLSDEVHARPPVPLDTPEFVTYLAFLHHEGSA
                     GREADHLAALAGQLGQSAPDIDSGHVLLEAEGFRLKWERHNEFSSYCFFRRIEPDDSP
                     DDYALLHVPAAWRKAIPGLLIAATHIELRDVGEVPLETVLHQQSRHGQALVASGVSDG
                     AGWVLTDFHLHDGFSHFLLLDKGFTPRQAGRIAQRLVEIETYRVMALLAFPVAKDTGR
                     LVSRAEDELADLMDGMGRSRSAEDDRAVLARLSRLAAEVERSVARTTFRFGAAAAYYR
                     LVRQRIDDLREQRLPGFSPIGEFMDRRLVPAIDTCTSIARRQDDLSGRIARNSQLLRT
                     RVDIEIERQNQELLAQMNRRAKIQLHLQETVEGLSVVAITYYASQLVNYVAKGAKHLI
                     EPLTPEVLTAVSIPVIAGIVFMGLRRMRKLLGTEEDGQAHP"
     gene            complement(4151..5215)
                     /gene="fba"
                     /locus_tag="PKN25_11240"
     CDS             complement(4151..5215)
                     /gene="fba"
                     /locus_tag="PKN25_11240"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929444.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HNV91517.1"
                     /translation="MPLVSMRQLLDHAAEHSYGLPAFNVNNLEQVQAIMEAAAECDAP
                     VIMQASAGARKYAGEAFLRHLIDAAIEAYPHIPVVMHQDHGQSPAICMGAIRSGFSSV
                     MMDGSLLEDGKTPSSYEYNVAVTREVVKFSHAIGVSVEAELGCLGSLETGQAGEEDGV
                     GAEGTLDHSQMLTDPDQAADFVKQTDCDALAIAIGTSHGAYKFTRKPTGDILAIERVK
                     AIHARIPNTHLVMHGSSSVPQELLEIIRQYGGDMKETYGVPVEEIVQGIKYGVRKINI
                     DTDIRLAMTGAIRKFMFENPSKFDPREYNKPAREAAKLVCKARYEAFGCAGMASKIKP
                     VALDKIAARYKAGELSQIVR"
     gene            complement(5323..6777)
                     /gene="pyk"
                     /locus_tag="PKN25_11245"
     CDS             complement(5323..6777)
                     /gene="pyk"
                     /locus_tag="PKN25_11245"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNV91518.1"
                     /translation="MSRHTKIVATLGPASSDLLTLERMVHAGLDVVRMNFSHGKPEDH
                     VARAETIRDASARAGRPIGILADLQGPKIRVGKFEEGRVTLTRDAPFILDARCELGNV
                     QRVGLDYKDLPKDVKPGDVLLLDDGRLKLTVQRVLGHEIHTTVKVGGELSNNKGINRQ
                     GGGLTAPALTAKDMDDIRTAALIGVDFVAVSFPKSAADMYMARQLMRAAGCSALLIAK
                     IERTEAVANLDEILDASDGIMVARGDLAVEVGDAAVPALQKKMIRAARDRNKLTITAT
                     QMMESMITSPVPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPVEVVEAMSRVCLEAE
                     KSYDTGLDREMLDRVFTRIDQSIAMAAIWTAYHLKVKAIASLTQTGSTALWMSRLIAG
                     VPIYALTPEVSARNQMTLYREVHPLLMSQTHQDRDVLLWEAEQVLLEQGVVDYGDLIV
                     LTIGEPIGSAGGTNTLKIVRVGEHHRPGTLDDPV"
     gene            6859..8073
                     /locus_tag="PKN25_11250"
     CDS             6859..8073
                     /locus_tag="PKN25_11250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNV91519.1"
                     /translation="MRIGLHGIYWMKSVRGWRPGTGASLATHWRSVMALPGKPSRRTA
                     AMAVSSVVLCMLSASAAAVPSSLYVFGDSLSDNGNLYAWTSNPSNPVTFGLPIPVSPP
                     YEQGTFTNGPVYAERLWDRLGLPGELAPAALPGGTNHAVGGARSRYHSFDVGPDGLPP
                     LVWPANFASFSLTGQFQGYRDGLAGGAADASALYLVWAGANDLQDVLRLAQLSGPAAA
                     GARLAEAVGDVAGVIGGLVGLGARQLLVPTVPDIGALPVVAAFGAQAQGIARQYSIAF
                     NQAIDQALQSFAGIPALEVLRFDAFGLLDEVVRDPAANGFTDIDTACLQGLYIAPTPG
                     APAPTICSNPDEHLFWDVVHPSAAAHVILADEMYREVRTAFVPEPPLPALLVVGALFL
                     GVFRRLRLTRPL"
     gene            8260..9024
                     /locus_tag="PKN25_11255"
     CDS             8260..9024
                     /locus_tag="PKN25_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase cytochrome b subunit"
                     /protein_id="HNV91520.1"
                     /translation="MTLPRPCAASAASPWPARLDLLQSLSGLALALFMWLHMGFVSAI
                     LISADFAWAVARFFEGYFFLPRPQAWLVTAFVASIALLFALHALLALRRFPADWRQYR
                     IAARHGSRLRHGDTTLWFVQVATGFAMFFLAPVHLYAMFMHPDLIGPYESADRVWTGG
                     FWPLYLALLFAVEVHGSVGLYRLAVKWGWFEGRDARTTRRRLKLAMWGLIAFLLTLGL
                     TTLLAEIRMGIEHAPRAGERYVPTWQRSPTAVEEAR"
     gene            9021..11024
                     /locus_tag="PKN25_11260"
     CDS             9021..11024
                     /locus_tag="PKN25_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase flavoprotein subunit"
                     /protein_id="HNV91521.1"
                     /translation="MRIEYTDVLVVGGGLAGLRTAVGAARRRLRVEVLSLVPAKRSHS
                     VAAQGGMQASLGNAFKGMGDNEDVHFGDTVRGSDWGCDQLVARMFAHMAPKAVRELAA
                     WGVPWNRVQRGAHEVVINGERVTLTERDEAHGLITARDFGGTRKWRTCYTADGTGHTM
                     LYALGDRAVAERVPVRDRSEAVALIHDGGRCQGAVVRDLETGELYAILAAATVIATGG
                     FGRIWGISTNAIINEGMGAAIALETGVARLGNMEAVQFHPTAIVPAGILVTEGCRGDG
                     GLLRDVDGHRFMPDYEPEKAELASRDVVSRRMAEHMRRGKGVKSAAGEHLWLDIAVLG
                     AAHIDHKLREVKDICRHFLGIDPARDWIPVRPTQHYSMGGIRTDHRGESPALRGLFAV
                     GEAACWDLHGFNRLGGNSLAETVVAGMLVGEYVADFVESHAGALQFSPGLAREFVGRE
                     RAALEAITARPAPAPGADNVASLRRGMEATMTEHVGLFRNEAGLRTAVATLVDLHARA
                     QRVGIASRHLDANPELVAAYRLPRMLALALCAAQGALERRESRGAHAREDYPARNDRD
                     WLRRTLAWWPVGAATPVLEYEPIRVESMELPPGFRGYGTRNIVEHPATALREAELESI
                     RARLEGAGGAPLQAALLDFRARLPERYRGDNERIADIEEGAPR"
     gene            11021..11758
                     /locus_tag="PKN25_11265"
     CDS             11021..11758
                     /locus_tag="PKN25_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HNV91522.1"
                     /translation="MSRRLKISIFRHNPRDPRSVPRLVDYELDERPGMTLFIALNAIR
                     DTQAPDLNFDFVCRAGVCGACAMVIDGRPGLACRTLTSTLPAAFSLYPLPVFRLVADL
                     SVDTGSWMRELAERLQTWVHLDAEPDLDRLEARMEPALAEAVYELDRCIECGCCIAAC
                     ATAQMREGFVGAVGLVKIARFRLDSADTRDDADFYELVGNDEGIFGCMSLLACNDLCP
                     KELPLQTQLAWLRRKMAGQGVRWLRRG"
BASE COUNT         1602 a         3952 c         4384 g         1880 t
ORIGIN      
        1 cccggccgca tcagtcggcg tcgatgcaga aggagcgctg gaatcatcaa acgccagaaa
       61 cagggagctt cgccatggca ggtttcgaca tcaggaacga tacgatctgg ccgctggaga
      121 tctcgctcgg aatgctgagt ccgctgtact ggggcttgac cgagcccgcg cagaccttct
      181 ccagaagcac gggcgcggtc tggttcacca ttcaggccac ggtggcgctg gaccacaagg
      241 agcacatcac gacggccggg gcggtcttcg atgtcttcaa gaacatcctc ttcccgcagg
      301 tgatgctcgg caattggctc atcaatggcg aaatgctcgc cggatcgcat ggcatcaccg
      361 aggacgtggc ccaggccctg ggcgcaaagc ggccgatcac gatcaagggc cggccgctcg
      421 acgagatgtc gaccgaagat gcgatcgcgg gtttgcaggg aatcttcacc catgacaggt
      481 cgcgcgtctc gaaggcggga tgctatgcgg gctggagcgg gcacaagcag tacgtcgtca
      541 gcggcgggcc gacgctgcag gtcagggctg gcggcgtgga actcgtgtcg ggtacgccgc
      601 tgtccatcgt gaagctgtag gcgcgccgag accttggcac cggaccgcga cgtcgtcacg
      661 gtgaatgtcg cgtgtgctcg tagcacgagg acgaaagacc gcgaacggcc cggcctgcgg
      721 gtctcccgga cagcctcagg ccgtgctcgc ctcgaacagt cccgccagcg ccggcgccag
      781 cacgaagtcg aactccgccg ccagcgtggc gaccggatcg tccgccgcga cgaagtccgc
      841 cagcagcagc gggcgcagcg cctcgggcac gcgcatgaag aggccgtcgc gctggttgag
      901 cggttcgccg gcgacataga tctgggtgac gaagggacgc ccgccttcct ggaacaccgc
      961 ggcgtggatg tgcggcgtgc gtcctgggta gggcaccggg cggatggtgc ggaagcggta
     1021 gcgcccgtcg gcgtcggtga gggtgtggcc gaagccctgg aagtccgggt cctcctccac
     1081 gtcggcgcga tcgcgcgggt ggcggtagcg gccgttggcg tcgcactgcc agatttccac
     1141 gcgcaggcca gccagcgggc ggccgtcgag gtcgagcacg cggcccgaca ggtcggcgat
     1201 ctgcccggcg gcacgaccgc tgcggccttc gacccgggtg aggtcgttgt ccttgtgcag
     1261 cggcggggtg accgggtaga aggggccggg catctggctc ggcgtcagca cgcgctgcgc
     1321 cgcggcgagc ggcgcggaga gcagggtggc gctgccgccg aggacgaggc ggcggcggag
     1381 tccggaatgg tgcgacatgc ttgcggctcc ggctgtggac atgccgtgaa gaccgtcgcg
     1441 gtggggcgcg gttccgcgcg tgcccggacg cgcgcgtcgg gcttggcggg cggtgggggc
     1501 gcaggcggcg caggcggtct cgcgcctgcc cgcaggcgcg tccagactgc cttggagatt
     1561 gggtggtcgg acggaggcgg ggcatggagc gaagacggtt catcggcgcc gcggcgctgg
     1621 ccgggctggg ggtggtgggc ggggccggcg tcctttccca tcggcagagc gtggcggtct
     1681 acgaggcagc agcgggtacg acgtggcgcc atggcgagcc ggcgcagcgg gacacgcgtg
     1741 cggcactgcg cgagctcgtg cgctacgcca cccttgcgcc gtccagccac aacacgcagt
     1801 gctggaagtt cgcgctggcg gagcggtcgg tttccattct ccccgactac tcgcggcgct
     1861 gcccggtggt ggatcccgac gatcaccacc tcttcgtcag cctgggctgc gctgccgaga
     1921 acatcgtgca ggcggcgccg gcgatgggct tccactgcga ggccgccttc gacggggagg
     1981 ccgccgaggt cctgaactgc gcgctggcgc ccgcgccgag ccgacgaacc gccctgttcg
     2041 aggccattcc gcagcgtcaa tcgacccgcg gcgagttcga ctcgcggccg ctttcgaacg
     2101 aggagctgcg gcggctcgag gcggccggct cgggcagggg cgtgagcgtg cgcctgctca
     2161 ccggggcgca ggcgttggaa cgcgtcctgg agttcgtcgt cgcggggaac acggcgcaga
     2221 tgcgggaccc ggcgttcgtg cgcgagctga aggcgtggat ccgcttcaac ggcagcgatg
     2281 cggcgcgcac cggcgacggc ctgttcgcag gggcgtccgg aaatcccgcg gcccccgcct
     2341 ggctgggcgg cctgctgttc gatgccttct tcaccgaggc ggccgagaac gacaagtacg
     2401 cgcggcagat ccgcagtgct gcggggatcg cggtgttcgt ctcggagcgc gacgatcgcg
     2461 cgcactggat cgaggcgggg cgttgcttcc agcgctttgc gctgcaggcg accgcgatcg
     2521 gcgtccgcac ggcgcatctc aaccagccgg tcgagctcgc cacgctgcgc cccgcctttg
     2581 ccgccgacct gggcatcacg aacggccggc cggatctggt gatccgtttc ggcagggggc
     2641 cggcgatgcc ccgatccctg cgccgtccgc tcgacgccgt gctgctgtga ggggcggcgc
     2701 ccgccgccgg ccacctcaag ggtgggcctg gccgtcctct tccgtgccca gcagcttgcg
     2761 catccgccgc agccccatga agacgatgcc ggcgatgacc gggatcgaca ccgcggtgag
     2821 cacctccggc gtcagcggct cgatcaggtg ctttgcgccc ttggcgacgt agttcaccag
     2881 ctgcgaggcg tagtaggtga tcgccaccac cgacagccct tcaacggtct cctgaaggtg
     2941 gagctggatc ttggcgcggc ggttcatctg cgcgagcagc tcctggttct ggcgctcgat
     3001 ctcgatgtcg acgcgggtgc gcagcagctg cgagttgcgt gcgatgcggc cggagaggtc
     3061 gtcctggcgg cgcgcgatcg aggtgcaggt gtcgatcgcg gggacgaggc ggcggtccat
     3121 gaactcgccg atcggcgaga agccgggcag gcgctgttcg cgcaggtcgt cgatgcgctg
     3181 gcgcaccagg cggtagtagg ccgcggcggc gccgaagcgg aaggtggtgc gtgccaccga
     3241 gcgctcgacc tcggcggcca ggcgggacag gcgggcgagc accgcgcggt cgtcctcggc
     3301 gctgcgcgac cggcccatgc cgtccatgag gtcggcgagc tcgtcctcgg cgcgcgagac
     3361 caggcggccg gtgtccttgg ccaccgggaa ggcgagcagc gccatgacgc ggtaggtctc
     3421 gatctcgacc aggcgctggg cgatgcggcc ggcctggcgc ggggtgaagc ccttgtcgag
     3481 cagcaggaag tgcgagaagc cgtcgtgcag gtggaagtcg gtgagcaccc agccggcgcc
     3541 gtccgagacg ccggaggcga ccagcgcctg gccgtggcgc gactgctggt gcagcaccgt
     3601 ctccagcggc acctcgccga cgtcgcgcag ctcgatgtgg gtggcggcga tcagcaggcc
     3661 ggggatggcc ttgcgccagg ccgcgggcac gtgcagcagg gcgtagtcgt cggggctgtc
     3721 gtcgggctcg atgcggcgga agaagcagta gctggagaac tcgttgtggc gctcccactt
     3781 cagccggaaa ccctcggcct cgagcagcac gtggcccgag tcgatgtccg gcgcggactg
     3841 gccgagctgc ccggccagcg ccgcgaggtg gtccgcctcg cgcccggcgc tgccttcgtg
     3901 gtgcaggaag gcgaggtagg tgacgaattc cggggtgtcc agcggcaccg gcgggcgcgc
     3961 gtgcacctcg tcgctgagcg tctggcgcag ggggtgctgt tcgaagaggg aggacatggt
     4021 ggctcgcaga gcagtcggcg gcgggttgga tgagggcgac gcgatcgaag acaatggatc
     4081 cacggccgct tgaagcgacg accgcgccag ctggcgcggt cgtcgcttca agcatgtgct
     4141 ccgcgctggt tcagcgcacg atctggctca gctcgccggc cttgtaacgc gccgcgatct
     4201 tgtcgagcgc gacgggcttg atcttcgagg ccatgccggc gcagccgaag gcttcgtagc
     4261 gcgccttgca gaccagcttg gcggcctcgc gcgcgggctt gttgtactcg cgcgggtcga
     4321 acttcgacgg gttctcgaac atgaacttgc ggatcgcccc ggtcatggcg aggcggatgt
     4381 cggtgtcgat gttgatcttg cgcacgccgt acttgatgcc ctggacgatc tcctcgaccg
     4441 gcacgccgta ggtctccttc atgtcgccgc cgtactggcg gatgatctcg aggagctcct
     4501 gcggcaccga ggaggagccg tgcatcacca gatgggtgtt ggggatgcgg gcatggatgg
     4561 ccttgacgcg ctcgatggcg aggatgtcgc cggtgggctt gcgggtgaac ttgtaggcgc
     4621 cgtggctggt gccgatggcg atggcgagcg cgtcgcagtc ggtctgcttg acgaagtcgg
     4681 cggcctggtc ggggtcggtg agcatctggc tgtggtcgag cgtgccctcg gcgccgacgc
     4741 cgtcttcctc gccggcctga ccggtctcga gcgagccgag gcagccaagc tcggcctcga
     4801 cgctgacgcc gatcgcgtgc gagaacttga ccacctcgcg ggtgacggcg acgttgtatt
     4861 cgtaggagga gggcgtcttg ccgtcttcca gcagcgagcc gtccatcatc acgctcgaga
     4921 agcccgagcg gatcgcaccc atgcagatcg ccggcgactg gccgtgatcc tggtgcatga
     4981 cgaccgggat gtgcggatag gcctcgatcg cggcatcgat gaggtggcgc aggaaggctt
     5041 cgcccgcgta tttgcgcgcg ccggccgagg cctgcatgat caccggagcg tcgcactcgg
     5101 cggccgcttc catgatcgcc tgcacctgtt cgaggttgtt cacgttgaag gcgggcaggc
     5161 catagctgtg ttcggcggcg tggtcgagca gctggcgcat ggaaacgagg ggcatggaat
     5221 ctctccggaa aaggccgccg ggcggggcgg ccggacgatc gaaaattcta ttttagcgtg
     5281 tgcgcgggga cgctgaccgt gtctcccgtc gcggacgggt attcagacgg ggtcgtcgag
     5341 cgtgccgggg cggtggtgtt cgcccacgcg cacgatcttg agcgtgttgg tgccacccgc
     5401 gctgccgatc ggctcgccga tggtgagcac gatgaggtcg ccgtaatcga ccacgccctg
     5461 ctcgagcagg acctgctccg cctcccacag cagcacgtcg cggtcctggt gggtctggct
     5521 catcagcagc ggatggacct cgcggtagag cgtcatctgg ttgcgcgccg agacctcggg
     5581 ggtgagcgcg tagatcggca cgccggcgat caggcggctc atccacagcg cggtcgagcc
     5641 ggtctgggtg agcgaggcga tcgccttgac cttgagatgg taggcggtcc agattgccgc
     5701 catcgcgatc gactggtcga tgcgggtgaa cacgcggtcg agcatctcgc ggtcgagccc
     5761 ggtgtcgtag gacttctccg cctccaggca gacccggctc atggcctcga ccacctccac
     5821 cgggtacttg ccggcggcgg tctcggccga cagcatcacc gcgtcggtgc cgtcgagcac
     5881 cgcgttggcc acgtcggaga cctcggcgcg ggtgggcacc gggctggtga tcatggattc
     5941 catcatctgc gtggcggtga tggtgagctt gttgcggtcg cgcgcggcgc ggatcatctt
     6001 cttctgcagc gccggcaccg cggcgtcgcc cacctccacc gcgaggtcgc cgcgcgccac
     6061 catgatgccg tcggaggcgt cgaggatctc gtcgaggttg gccaccgcct cggtgcgctc
     6121 gatcttggcg atcagcagcg ccgaacagcc ggcggcgcgc atgagctggc gcgccatgta
     6181 catgtcggct gcgctcttgg ggaaggacac cgcgacgaag tccacgccga tcagcgcggc
     6241 ggtgcggatg tcgtccatgt ccttggcggt gagcgcgggc gcggtgagcc cgccgccctg
     6301 gcggttgatg cccttgttgt tggacagctc gccgcccacc ttcaccgtgg tgtggatctc
     6361 gtggccgagc acgcgctgca cggtgagctt gaggcggccg tcgtcgagca gcagcacgtc
     6421 gcccggcttc acgtccttgg gcaggtcctt gtagtcgagg ccgacgcgct ggacgttgcc
     6481 gagctcgcag cgggcgtcga ggatgaaggg cgcgtcgcgg gtcagcgtca cgcggccttc
     6541 ctcgaacttg ccgacgcgga tcttggggcc ctgcaggtcg gcgaggatgc cgatcgggcg
     6601 gccggcgcgc gcggaggcgt cgcggatggt ctcggcgcgg gcgacgtggt cctcgggctt
     6661 gccatgggag aagttcatcc gcaccacgtc gaggccggcg tgcaccatgc gctcgagggt
     6721 gaggaggtcg gacgaggcgg ggccgagggt ggcgacgatc ttggtgtggc gggacatccg
     6781 ggctgctccg gtcggtggcg tgcccgcatt ctagcagcgc ggccgcgacg gcgacttcgc
     6841 gcgcaccgta cgcgaggctt gcggatcggc ctgcatggca tatattggat gaagtctgtt
     6901 cgcggatggc gcccgggtac cggagccagc ctcgccaccc actggaggag cgtcatggcc
     6961 ctacccggca aaccgagccg tcgcacagcc gcgatggcgg tgtcgagcgt cgtgttgtgc
     7021 atgctgtccg caagcgcggc ggccgtgccg tccagcctgt acgtgttcgg cgacagcctc
     7081 tcggacaacg gcaacctgta tgcctggact tcgaatccgt cgaatccggt gaccttcggc
     7141 ttgccgatcc cggtgtcgcc cccctatgag cagggaacct tcaccaatgg cccggtatac
     7201 gcggagcgcc tttgggacag gctcggcctt cccggggagc tcgctccggc ggcgctgccg
     7261 ggcggcacga accatgcggt aggcggggcg cgctcgcgct accactcctt cgatgtcggc
     7321 cccgacggcc tgccgccgct cgtgtggccc gccaacttcg cgtccttcag cctgaccgga
     7381 cagttccagg gctaccgcga cgggctcgcc ggtggcgctg cggacgcgtc ggcgctgtat
     7441 ctggtctggg ccggtgcgaa cgacctgcag gacgtgctcc ggctcgccca gctgagcggc
     7501 cctgccgcag ccggtgcgcg gctggccgag gcggtcggcg atgtggccgg cgtgatcggc
     7561 gggctggtcg gcctgggcgc gcgccagctc ctcgtgccca cggtgccgga catcggcgcg
     7621 ctgccggtgg tggccgcctt cggtgcgcag gcccagggaa tcgcccgcca gtacagcatc
     7681 gcgttcaacc aggcgatcga ccaggccttg cagtccttcg ccggcatccc cgcactcgag
     7741 gtgctgcgct tcgatgcgtt cgggctgctc gacgaggtcg tccgcgatcc cgcggcgaac
     7801 ggcttcaccg acatcgacac ggcctgcctg caggggctgt acatcgcgcc gacgcccggt
     7861 gcgcccgcgc cgacgatctg ctcgaatccg gacgagcatc tgttctggga cgtggtgcat
     7921 ccgtcggcgg ccgcccacgt gatcctcgcg gacgagatgt accgcgaggt gcgcaccgcc
     7981 ttcgtgcccg agcccccgct gccggcgctg ctcgtggtgg gcgcgttgtt cctcggcgtc
     8041 tttcgtcgcc tgcggctgac caggccgctg taggcgtcgg gagcgcgcag cttcgtgcgc
     8101 ggcggcgcgc ttcgcctttc gggctgttgc attgcggcaa cggggggcgt gacttaatcc
     8161 actgagggag actttgcgcg gcgtcgggcg cggcgatctg ggttgtgcac tgcggcatcc
     8221 gttgtatagt gtgctgcctg tcctgcacat cgcatcttca tgacgctccc gcgaccttgc
     8281 gcagcttcgg ccgcctcgcc ctggccggca cgcctggatc tgctccagag cctgtccggc
     8341 ctcgccctcg ccctcttcat gtggctgcac atgggcttcg tgtccgcgat cctgatcagc
     8401 gcggacttcg cgtgggcggt ggcgcgtttc ttcgagggct acttcttcct gccgcgtccg
     8461 caggcgtggc tggtgacggc cttcgtcgct tcgatcgcgc tcctgttcgc cctgcatgcg
     8521 ctgctggcgc tgcgcaggtt tcccgccgac tggcgccagt accgcatcgc cgcgcgccac
     8581 ggcagccgtc tgcgccatgg cgacacgacg ctgtggttcg tgcaggtggc gaccggcttc
     8641 gcgatgttct tcctcgcgcc ggtgcatctg tacgccatgt tcatgcatcc cgacctcatc
     8701 ggcccttacg aatccgccga tcgcgtgtgg accggcggct tctggccgct ctatctcgcc
     8761 ttgctgttcg cagtcgaggt gcatggcagc gtgggcctgt accgcctggc ggtgaagtgg
     8821 ggctggttcg agggccgcga cgcgcgcacg acgcggcgcc ggctgaagct ggcgatgtgg
     8881 ggcctgatcg ccttcctgct cacgctgggg ctgaccacgc tgttggcgga gatccgcatg
     8941 ggcatcgagc atgcgccgcg cgccggcgag cgctacgtac cgacctggca gcgcagcccg
     9001 actgcggtgg aggaggcgcg atgaggatcg aatacaccga cgtgctggtg gtgggcggcg
     9061 gcctggccgg cctgcgcacc gcggtgggcg cggcaaggcg ccggctgcgg gtcgaggtgc
     9121 tgtcgctggt gccggccaag cgctcgcact cggtggcggc gcagggcggc atgcaggcga
     9181 gcctgggcaa cgccttcaag ggcatgggcg acaacgagga cgtgcacttc ggcgacaccg
     9241 tgcgcggctc ggactggggc tgcgaccagc tcgtcgcgcg catgttcgcc cacatggcgc
     9301 ccaaggcggt gcgcgagctc gcggcctggg gcgtgccctg gaaccgcgtg cagcgcggcg
     9361 cccacgaggt ggtcatcaac ggcgagcgcg tcacgctgac cgagcgcgac gaggcccacg
     9421 gcctcatcac cgcgcgcgac ttcggcggca cgcgcaagtg gcgcacctgc tacaccgccg
     9481 acggcaccgg ccacaccatg ctctacgcgc tcggcgaccg cgcggtggcc gagcgcgtcc
     9541 cggtgcgcga ccgcagcgag gcggtggcgc tgatccacga cggcgggcgc tgccagggcg
     9601 cggtggtgcg cgacctggag acgggcgagc tgtacgccat cctggccgcc gccacggtga
     9661 tcgccacggg cggcttcggc cgcatctggg gcatctccac caacgcgatc atcaacgagg
     9721 gcatgggtgc tgcgatcgcg ctcgagaccg gggtggcgcg cctgggcaac atggaggcgg
     9781 tgcagttcca ccccaccgcg atcgtgcccg ccggcattct cgtcaccgag ggctgccgcg
     9841 gcgacggcgg cctgctgcgc gacgtcgacg gccaccgctt catgcccgac tacgagccgg
     9901 agaaggccga gctggcctcg cgtgacgtgg tctcgcgccg catggccgag cacatgcgcc
     9961 gcggcaaggg cgtcaagtcg gccgccggcg agcacctgtg gctggacatc gcggtgctcg
    10021 gcgccgccca tatcgaccac aagctgcgcg aggtgaagga catctgccgc cacttcctcg
    10081 gcatcgaccc ggcgcgcgac tggatcccgg tgcggccgac ccagcactac tcgatgggcg
    10141 gcatccgcac cgaccaccgc ggcgagtcgc ccgcgctgcg cggcctgttc gcggtgggcg
    10201 aggcggcgtg ctgggacctg cacggcttca accgcctggg cggcaactcg ctcgccgaga
    10261 cggtggtggc gggcatgctg gtgggcgagt acgtcgccga cttcgtcgag tcgcacgccg
    10321 gcgcgctgca gttctcgccc gggctggcgc gcgagttcgt cggccgcgag cgcgctgccc
    10381 tggaggcgat cacggcgcgt ccggcgcccg cgccgggcgc ggacaacgtc gcctcgctgc
    10441 ggcgcgggat ggaggcgacc atgaccgagc acgtcggcct gttccgtaac gaggcggggc
    10501 tgcgcacggc ggtcgccacg ctggtcgacc tgcacgcgcg cgcgcagcgg gtcggcatcg
    10561 ccagccgcca cctcgacgcc aatccggagc tggtggccgc ctaccggctg ccgcgcatgc
    10621 tggcgttggc gctgtgcgcc gcgcagggcg cgctcgagcg ccgcgagagc cgcggtgcgc
    10681 acgcgcgcga agactacccg gcacgcaacg accgcgactg gctgcggcgc acgctggcct
    10741 ggtggccggt gggcgccgcc acgccggtgc tggaatacga gccgatccgg gtcgagagca
    10801 tggagctgcc gcccggattc cgcggctatg gcacgcgcaa catcgtcgag catccggcca
    10861 ccgcgctgcg cgaggccgag ctcgagtcca tccgtgcccg tctcgaaggc gcggggggtg
    10921 ccccgctgca ggccgcgctg ctggatttcc gcgcacgcct gcccgaacgc tatcgaggcg
    10981 acaacgaacg catcgccgac atcgaagagg gagcgccgcg atgagccgcc gcctgaagat
    11041 ctcgatcttc cgccacaacc cgcgcgaccc ccggagcgtg ccgcgcctgg tcgactacga
    11101 gctcgacgag cggccgggca tgactctctt catcgcgctc aacgcgatcc gcgacacgca
    11161 ggcgcccgac ctcaacttcg acttcgtgtg ccgcgccggc gtgtgcgggg cgtgcgcgat
    11221 ggtgatcgac ggccgccccg ggctggcctg ccgcacgctg acgagcacgc tgccggcggc
    11281 gttcagcctc tatccgctgc cggtgttccg cctcgtggcg gacctgtcgg tggataccgg
    11341 gagctggatg cgcgagctcg ccgagcgcct gcagacctgg gtgcatctcg acgcggagcc
    11401 cgatctcgat cgcctggagg cgcgcatgga gcccgcgctg gccgaggcgg tctacgagct
    11461 cgaccgctgc atcgaatgcg gctgctgcat cgccgcctgc gccaccgcgc agatgcgcga
    11521 gggcttcgtc ggcgcggtcg gtctcgtgaa gatcgcgcgc ttccgcctcg actcggcgga
    11581 tacgcgcgac gacgccgact tctacgagct cgtcggcaac gacgagggca tcttcggctg
    11641 catgagcctg ctcgcctgca acgacctctg tcccaaggag ctgcccctgc agacccagct
    11701 cgcctggctg cgccgcaaga tggccgggca aggggtacgc tggctgcggc ggggatgaat
    11761 cgagcgccgc aatcgcgact cgcgtcgctc ctacacctca tccgcggcgc cgcaggac
//
LOCUS       DAOQBS010000101        11752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4626_length_11752_cov_16.406258, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000101 DAOQBS010000000
VERSION     DAOQBS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11752
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4626_length_11752_cov_16.406258"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PKN25_11270"
     CDS             complement(<1..710)
                     /locus_tag="PKN25_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV91523.1"
                     /translation="MSTAFVVLALSLLLGLQPVTTDLYLPALPSITQDLGASVAQAQL
                     TLSGLLLAFGLSQLVWGPISDRFGRRPVLLLGLAIYVVAAVGSTLAGSMEALLLWRIA
                     QGAAMGASVMCARALVRDLYSPEVGARAISKGLTGLGVIACLSIPLGGVLAEFFGWRS
                     ALSALALFGAATLALIALRFEETLQRPDPDALRPATLVRTWITILRNPTFLAFALLAA
                     ATYGGLFTLLAASSFVFIR"
     gene            complement(892..1536)
                     /gene="maiA"
                     /locus_tag="PKN25_11275"
     CDS             complement(892..1536)
                     /gene="maiA"
                     /locus_tag="PKN25_11275"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HNV91524.1"
                     /translation="MKLYTYFRSSAAYRVRIALNLKGLDYEAVPVHLVRGGGEHRQPA
                     YLGLNPAGLVPALEDQGQVLTQSLAIIEYLEESHPQPALLPAAPLDRARVRAIAQAIA
                     CDIHPVNNLRVLQYLTRELDASEEQKNAWYRHWIGVGLQAVEAMLAGDARTGAFCHGD
                     TPGLADCCLVPQVFNARRFGCELAAMPTVLRIAETCAGLEAFQRAAPEAQPDAE"
     gene            complement(1542..2552)
                     /locus_tag="PKN25_11280"
     CDS             complement(1542..2552)
                     /locus_tag="PKN25_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNV91525.1"
                     /translation="MKLATLKQGGRDGTLVVVSRDLTRCRAVPAIARTLQAALDDWQA
                     CEPQLRQVYEALNSGAVDAQPFDQTACASPLPRAYQWADGSAYINHVELVRKARNAEM
                     PPSFYTDPLMYQGGSDSFIGPRDAVVADEAWGIDFEAEVTVVTGDVPMGATPAEAAQA
                     IRLVMLVNDVSLRNLIPNELAKGFGFFQSKPASAFSPVAVTPDELGEAWRDAKVHLPL
                     VVHLNGKLFGKPEAGVDMTFDFGQLVAHVARTRELEAGSIIGSGTVSNKQGDLWGSSI
                     DHGGVGYCCLAEVRTYETIEQGKPATSFMRDGDVVRIEMFDRQGRNVFGTIENRVTAR
                     KG"
     gene            complement(2549..3100)
                     /locus_tag="PKN25_11285"
     CDS             complement(2549..3100)
                     /locus_tag="PKN25_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV91526.1"
                     /translation="MTPIVKKIHHVAYRCKDALETARWYEKHLGMKLVLSIAEDAVPS
                     TGEPDPYMHIFMDAGMGNVLAFFELPTRAPMGRDENTPAWTQHLAMEVDSMETLLATK
                     VRLESEGIQVVGPTDHALFQSIYFFDPNGHRLELACNTHRPGMLEALDKVKWDMLEEW
                     AQTKKAPRHAAWMHDGRYKEMFQ"
     gene            complement(3254..3490)
                     /locus_tag="PKN25_11290"
     CDS             complement(3254..3490)
                     /locus_tag="PKN25_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HNV91527.1"
                     /translation="MALNTQPNLFEPGKRYFRAFSPGDDFYEALIETHRDLSDEQSEM
                     VNARLILLLANHIGDIAVLREAMSIARAGVVAKD"
     gene            complement(3502..5169)
                     /locus_tag="PKN25_11295"
     CDS             complement(3502..5169)
                     /locus_tag="PKN25_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV91528.1"
                     /translation="MLSTYQYPKFEYVRPPEIAEGRDGHYPVVIVGAGPVGLCTAIDL
                     ARQGRQVLLLDNDDTVSIGSRGVCYAKRTLEVLDRLGCGEEMVQKGVSWNVGRTFFRE
                     DEVFNFNLCPEPDHHRPGMINLQQYYLEDYLVQRARKLPNLEMRFRNNVIAVTPGEDK
                     ASLRVETPDGAYTLTTDWLVVADGARSPIRTMLGLDIEGKVFMDRFLIADVVMKADFP
                     TERWFWFDPPFHPNQSVLLHRQADNVFRIDFQLGWQADPEQEKKPENVIPRIQAMIGD
                     DREFELEWVSVYTFQCRRMNRFNHGRVLFVGDSAHQVSPFGARGANSGIQDADNLVWK
                     LALVMDGKAPATLLDTYGDERGFAADENIMNSTRSTDFITPKSRTSRTFRNAVLGLAE
                     HHPFARALVNSGRLSVPSFLVDSRLNTADDAVFAGNMVPGAPMDDAPIESVGGEPWLL
                     QVLGDRFQLLHYVADAADLDAASVAALQSLAEGPIPVEPIVVAARGRAPAGLRTLIDA
                     KGRIRERYDLQPGTTYLARPDQHVAARWRALDPARVRAAVARATCNA"
     gene            complement(5325..6290)
                     /locus_tag="PKN25_11300"
     CDS             complement(5325..6290)
                     /locus_tag="PKN25_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV91529.1"
                     /translation="MSTKKFASQADLEEKKISWEKLSDNAYAYTAEGDPNTGVIIGDD
                     GVMIIDATATPVAAQGVIAKVREITDLPIKYVVLTHYHAVRVLGASGYKAEGLEQIIA
                     SKDTWDLIVERGQQDMDSEIGRFPRLFQAVESVPGLTWPTMTFSGEMTVWMGKLEVRI
                     KQLGRGHTKGDTIVYLPAQDICFSGDLVEADAACYTGDAYLADWPQTLDNLAAMNFAK
                     LVPGRGPALMTPEKVQAGLAYTRDFVSTLYRSAQEAVAKGLDLKATMAHTRANMDPKF
                     GQVFIYEHCLPFDVTRAHDEASGIRDPRIWTAERDQQMWHALQQG"
     gene            6437..7264
                     /locus_tag="PKN25_11305"
     CDS             6437..7264
                     /locus_tag="PKN25_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNV91530.1"
                     /translation="MQDIPNPSPSTARDAAESAAERRGIQSIEVGGSLLSALVREGKP
                     MMLKDLAREAGMPAAKAHPYLVSFGKLGLVEQDALTGRYGLGPFALQMGLSALHGLDP
                     IKAALPEAARLADDIELNVAIAVWGNHGPTVVHIEECSRQIHINMRPGTVMTPLLLSA
                     TGRVFAAFLPQRIVAPVLAAEAAAVGAPPLEMSPADVRAVLDEIRTQRLARALGNPIP
                     GIHAFSAPVFDGGGQLALAITAMGPVGSVDPDWEGLTACRVRAAAKAIERRLGKQAA"
     gene            7332..7946
                     /locus_tag="PKN25_11310"
     CDS             7332..7946
                     /locus_tag="PKN25_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91531.1"
                     /translation="MSSERFPAFFDQVPRLVVRDPLAEFLGAAEGGVLEYGYADAVRL
                     AGHSCPTVASAYALAHHAMTRLFPGALPVRGLVQVGFQHPLDEGVTGVVAAVVGLLTG
                     SAQDGGFKGIAGRFVRRNLQRFGEPQPLALRFSRSDTGASVDAAAELAKVQPHPEMAE
                     RMGRCMRGDADAGELQRFGQLWQDRVRRVLVEHWDDPEVFHFSG"
     gene            complement(8026..8397)
                     /locus_tag="PKN25_11315"
                     /pseudo
     CDS             complement(8026..8397)
                     /locus_tag="PKN25_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(8420..8857)
                     /gene="aroQ"
                     /locus_tag="PKN25_11320"
     CDS             complement(8420..8857)
                     /gene="aroQ"
                     /locus_tag="PKN25_11320"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNV91532.1"
                     /translation="MPKVLVINGPNLNLLGTREPEIYGATTLADVENGLRAQGEALGI
                     ELLCFQSNHEGAIVDRIHAARTEGVAWILINPGAYTHTSVAIRDALAGVAIPFVEVHI
                     SNVHKREPFRHHSYLSDVAEAVMAGFGVHGYGLALQYIALRLS"
     gene            complement(9062..10525)
                     /locus_tag="PKN25_11325"
     CDS             complement(9062..10525)
                     /locus_tag="PKN25_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNV91533.1"
                     /translation="MGKPTGFMEYQRLSEAYEPVDKRLKTYKEFVVRLTDEQASVQGA
                     RCMDCGIPFCNNGCPVNNIIPDWNDLVYRGHWREAIEVLHSTNNFPEFTGRICPAPCE
                     AACTLNINTDAVGIKSIEHAIIDKAWEEGWVVPQPAEVKTGKKVAVVGSGPAGLAAAQ
                     QLARAGHDVTVFEKNDRIGGLLRYGIPDFKMEKSLIDRRVEQLQAEGVVFRTGVLVGA
                     QNVPAGIVNDAKQVVSAEQLKQDFDAVVLAGGSEIPRDLPVPGRELGGVHYALEFLIP
                     QNKQVAGDKPNPISAKGKHVVVIGGGDTGSDCVGTSNRHGAASVTQFELMPMPPEQEN
                     KALTWPYWPTKLRTSSSHEEGCERDFAVATKEFIGKNGKVKALKACRLEFKDGKMSEV
                     PGSEFEIKADLVLFAMGFTQPVGGVLEAFGVDKDARGNAKASTDGAGSYTTNVPKVFA
                     AGDMRRGQSLVVWAIREGRQCARAVDEFLMGESLLPR"
     gene            complement(10526..>11752)
                     /locus_tag="PKN25_11330"
     CDS             complement(10526..>11752)
                     /locus_tag="PKN25_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNV91534.1"
                     /translation="GCIMMRKCHLNTCPVGVATQDPVLRARFSGQPEHVVNYFFFVAE
                     EVRELMAQLGVRTFDELIGRADLLDMKKGIAHWKAQGLDYSRIFYLPNVPAEVPRLHR
                     ETQDHGLDKALDRQLVVLAKPALEKGEKVNIDLPVRNINRTVGAMLSGEVAKRYGHAG
                     LPNDTIHIRLNGTAGQSFGAFLARGVTLELVGEGNDYVGKGLSGGRIIVRPKAEFRGD
                     TPQNIIVGNTVLYGATEGEVYFAGVAGERFAVRNSGATAVVEGVGDHGCEYMTGGTVV
                     VLGETGRNFAAGMSGGVAYVLDEDGTFEQRCNMAQVALEPLPEEIAARKGSESGDELE
                     SHGRVDIDHLTMGDELILKGLIERHVRFASSVRAREILNNWGLWRKKFVKVFPHEYRR
                     ALTEMAQQREAEKEAA"
BASE COUNT         1754 a         3985 c         4154 g         1859 t
ORIGIN      
        1 cggatgaaca cgaaggacga ggccgccagc agggtgaaca ggccgccgta ggtcgccgca
       61 gccagcagcg cgaaggcgag gaaggtgggg ttgcgcagga tggtgatcca ggtgcgcacc
      121 agcgtggccg ggcgcaacgc atccggatcg gggcgctgca gggtctcctc gaagcgcagc
      181 gcgatcagtg ccagcgtggc cgcaccgaac agcgccagcg ccgacaaggc gctccgccag
      241 ccgaagaact cggcgagcac gccgccgagc gggatgctca ggcaggcgat cacgcccagc
      301 ccggtgagcc ccttgctgat cgcgcgcgcc ccgacctcgg ggctgtagag gtcgcgcacc
      361 agcgcacgcg cgcacatcac cgacgccccc atcgccgcgc cctgggcgat ccgccacagc
      421 agcagcgcct ccatcgaccc ggcgagcgtg ctgcccaccg ccgccacgac gtagatcgcc
      481 agcccgagca ggagcaccgg ccggcgcccg aagcggtccg agatcggtcc ccacaccagc
      541 tgcgacaggc cgaaggcgag cagcaggccc gacagcgtga gctgcgcctg cgccaccgag
      601 gcgcccaggt cctgggtgat cgagggcagc gcgggcaggt agagatcggt ggtgaccggc
      661 tggaggccga gcagcagcga caaggcaagg acgacgaaag cggtcgacat cgggactccg
      721 aaaacgggga ccaagcccgc ggaagatacc gccggtggca ggacgaaggg caaaaaagat
      781 cgtcgccggg catccctgcg cgactcgcgc acgcgccccg gtctccctcg cctgcgcgcg
      841 cagccattcg caaccgctca cctgcaaggc cggaaagcca gactgcgccg ctcactcggc
      901 gtcgggctgg gcctcgggcg cggcgcgctg gaaagcctcc agcccggcgc aggtctccgc
      961 gatgcgcagc accgtcggca tcgccgccag ctcgcagccg aagcggcggg cgttgaagac
     1021 ctgcggcacc aggcagcagt cggccaggcc gggcgtatca ccgtggcaga acgcaccggt
     1081 acgcgcatcg ccggcgagca tcgcctccac cgcctgcagg ccgacgccga tccagtgccg
     1141 gtaccaggcg ttcttctgct cttcgctggc atcgagctcg cgcgtgaggt actgcagcac
     1201 gcgcaggtta ttcaccgggt ggatgtcgca ggcgatcgcc tgcgcgatcg cacgcacgcg
     1261 ggcgcggtcg agcggcgcgg ccggcagcag tgccggctgc ggatggctct cctcgagata
     1321 ctcgatgatc gccagcgact gggtcagcac ctggccctgg tcctccagcg ccggcaccag
     1381 gccggcggga ttgaggccca ggtaggcggg ctggcggtgc tcgccgccgc cgcgcaccag
     1441 gtgcaccggc acggcctcgt agtccaggcc cttgaggttc agtgcgatgc gcacgcgata
     1501 cgcggccgaa ctgcggaagt aggtgtagag cttcacttgc ctcagccctt gcgcgccgtc
     1561 acccggttct cgatcgtgcc gaagacgttg cggccctggc ggtcgaacat ctcgatgcgg
     1621 acgacgtcgc cgtcgcgcat gaaggacgtg gccggcttgc cctgctcgat ggtctcgtag
     1681 gtgcgtactt cggcgaggca gcagtagccg acgccgccgt gatcgatcga cgagccccac
     1741 aggtcgccct gcttgttcga gaccgtgccc gagccgatga tcgagcccgc ctcgagctcg
     1801 cgcgtcctgg cgacgtgggc gacgagctgg ccgaagtcga aggtcatgtc gacgccggcc
     1861 tcgggcttgc cgaagagctt gccgttgagg tgcaccacca gcggcaggtg caccttggca
     1921 tccctccagg cttcgccgag ctcgtcgggc gtgaccgcga ccggactgaa ggccgaggcc
     1981 ggcttgctct ggaagaagcc gaaacccttg gcgagctcgt tgggaatcag attgcgcagc
     2041 gacacatcgt tcaccagcat caccagccgg atcgcctgcg ccgcctcggc gggggtggcg
     2101 cccatcggca cgtcgccggt caccaccgtg acctcggcct cgaagtcgat gccccaggcc
     2161 tcgtcggcca ccaccgcgtc gcgcgggccg atgaagctgt ccgagccgcc ctggtacatc
     2221 agcgggtcgg tgtagaacga gggcggcatc tcggcattgc gcgccttgcg caccagctcg
     2281 acgtggttga tgtaggccga gccgtcggcc cactggtagg cgcgcggcag cggcgaggcg
     2341 caggcggtct ggtcgaaggg ctgggcgtcg accgcgccgc tgttgagcgc ctcatacacc
     2401 tggcgcagct gcggctcgca ggcctgccag tcgtcgagcg cggcctgcag ggtgcgggcg
     2461 atcgccggca cggcacggca gcgggtgaga tcgcggctga cgacgaccag ggtgccgtcg
     2521 cggccgcctt gcttgagggt ggcgagtttc actggaacat ctccttgtag cggccgtcgt
     2581 gcatccaggc cgcgtgcctg ggggccttct tggtctgcgc ccactcctcg agcatgtccc
     2641 acttgacctt gtcgagcgcc tccagcatgc cggggcggtg ggtgttgcag gcgagctcga
     2701 ggcggtggcc gttgggatcg aagaagtaga tcgactggaa gagcgcgtgg tcggtgggac
     2761 cgaccacctg gatgccctcg gactcgaggc ggaccttggt ggcgagcagg gtctccatcg
     2821 agtcgacctc catcgccagg tgctgggtcc acgccggggt gttctcgtcg cggcccatcg
     2881 gcgcacgcgt ggggagctcg aagaaggcga gcacgttgcc catgccggcg tccatgaaga
     2941 tgtgcatgta gggatcgggc tcgccggtag agggcacggc gtcctcggcg atcgacagca
     3001 cgagcttcat gccgaggtgc ttctcgtacc agcgcgcggt ctcgagcgcg tccttgcagc
     3061 ggtaggcgac gtgatggatc ttcttcacga tcggggtcat tgcggtctcc tcgtccttgg
     3121 ttcagtgccg aagctccgtt gcggcatttc gggaagataa cggagcgctc cggtccgggc
     3181 atcatcattg cagcgatccc gctcgctgca cgtcgtctgc agcacggcca ccatcgaccg
     3241 gctcgcgtaa cgatcagtcc tttgcgacca cccctgcgcg agcgatgctc atcgcctcgc
     3301 gcagcaccgc gatgtcgccg atgtggttgg cgagcagcag gatcaggcgg gcgttgacca
     3361 tctcgctctg ctcgtcgctc agatcacggt gggtctcgat cagggcttcg tagaaatcgt
     3421 cgccgggcga gaaggcacgg aagtagcgct tgccgggttc gaacaggttg ggctgggtgt
     3481 tcagggccat gacggtctcc ttcaggcgtt gcaggtggcg cgggcgacgg cggcgcgcac
     3541 gcgggccggg tcgagggcgc gccagcgcgc ggcgacgtgc tggtcggggc gggcgaggta
     3601 ggtggtgccg ggctgcaggt cgtagcgctc gcggatgcgg cccttggcgt cgatcagcgt
     3661 gcgcagtccg gccggcgccc ggccgcgcgc cgcgaccacg atcggctcca ccggaatcgg
     3721 cccttccgcg agcgactgca gcgcggccac gctggcggcg tcgaggtcgg cggcgtcggc
     3781 cacgtagtgc agcaactgga agcggtcgcc gagcacctgc agcagccagg gctcgccgcc
     3841 gacggactcg atcggggcgt cgtccatcgg cgcgcccggc accatgttgc cggcgaacac
     3901 cgcatcgtcg gcggtgttga gccgcgaatc gacgaggaag ctcggcaccg acaggcgccc
     3961 ggagttgacc agggcgcgcg cgaagggatg gtgctcggcc aggcccagca ccgcgttgcg
     4021 gaaggtcctg ctggtgcggc tcttgggcgt gatgaagtcg gtcgagcgcg tcgagttcat
     4081 gatgttctcg tccgccgcga agccgcgctc gtcgccgtag gtgtcgagca gcgtggcggg
     4141 cgccttgccg tccatcacca gcgcgagctt ccacaccagg ttgtcggcgt cctggatgcc
     4201 ggagttggcg ccgcgcgcgc cgaagggcga gacctggtgc gccgagtcgc cgacgaagag
     4261 cacgcgaccg tgattgaagc ggttcatgcg ccggcactgg aaggtataca cgctgaccca
     4321 ctcgagctcg aactctcgat cgtccccgat catcgcctgg atgcgcggga tcacgttctc
     4381 gggcttcttc tcctgctccg gatcggcctg ccagccgagc tggaagtcga tgcggaagac
     4441 gttgtcggcc tggcggtgca gcagcaccga ctggttggga tggaagggcg ggtcgaacca
     4501 gaaccagcgc tcggtgggga agtccgcctt catgaccacg tcggcgatga ggaagcggtc
     4561 catgaagacc ttgccctcga tgtccagccc gagcatggtg cggatcgggc tgcgcgcacc
     4621 gtccgcgacc accagccagt cggtggtgag cgtataggcg ccgtcgggcg tctccacgcg
     4681 caggctcgcc ttatcctcgc cgggggtgac cgcgatcacg ttgttcctga agcgcatctc
     4741 gaggttcggg agcttgcgcg cgcgctgcac caggtagtcc tcgaggtagt actgctgcag
     4801 gttgatcatg cccgggcggt ggtggtcggg ctcggggcag aggttgaagt tgaacacctc
     4861 gtcctcgcgg aagaaggtgc ggccgacgtt ccacgacacg cccttctgca ccatctcctc
     4921 gccgcagccg aggcggtcga gcacctcgag ggtgcgcttg gcgtagcaca cgccgcgcga
     4981 gccgatcgac accgtgtcgt cgttgtcgag cagcagcacc tgccggccct ggcgggcgag
     5041 gtcgatcgcg gtgcacagac ccaccgggcc ggcgccgacg atcaccaccg ggtagtggcc
     5101 gtcgcgccct tcggcgatct cgggcggacg gacgtattcg aacttggggt attggtacgt
     5161 gctcagcacg gcggtctcct ccgggtttgt tcgtgtgcgc cggggccgcg tcttggcgcg
     5221 cggtcctgcg gtgcggtgcg gggcgccgca gccggcgccg gacgctgctg gaaatcaggg
     5281 gtgcgagagg cgcggcgcca cacgacgccc tgcccgctgc aggctcagcc ctgctgcagc
     5341 gcgtgccaca tctgctggtc gcgctcggcg gtccagatgc gcggatcgcg gatgccgctc
     5401 gcctcgtcgt gggcgcgggt gacgtcgaag ggcaggcagt gctcgtagat gaagacctgg
     5461 ccgaacttcg ggtccatgtt ggcgcgggtg tgcgccatgg tcgccttcag atccaagccc
     5521 ttcgcgaccg cctcctgcgc gctgcgatac agcgtggaga cgaagtcgcg cgtgtaggcg
     5581 aggccggcct ggaccttctc cggcgtcatc agcgccgggc cgcggccggg cacgagcttg
     5641 gcgaagttca tcgctgccag gttgtcgagg gtctgcggcc agtcggcgag gtaggcgtcg
     5701 ccggtgtagc aggcggcgtc ggcctcgacc aggtcgccgg agaagcagat gtcctgggcg
     5761 ggcagataga cgatggtgtc gcccttggtg tggccgcggc cgagctgctt gatgcggacc
     5821 tcgagcttgc ccatccacac ggtcatctcg ccactgaagg tcatggtcgg ccaggtcagt
     5881 ccgggcacgc tctcgaccgc ctggaacagg cgcgggaagc ggccgatctc ggagtccatg
     5941 tcctgctggc cgcgctcgac gatgaggtcc caggtgtcct tgctggcgat gatctgctcc
     6001 aggccctcgg ccttgtagcc cgaggcgccg agcacgcgca ccgcgtggta gtgggtgagc
     6061 accacatact tgatcggcag gtcggtgatc tcgcgcacct tggcgatcac gccctgcgcg
     6121 gccaccgggg tggcggtggc gtcgatgatc atcacgccgt cgtcgccgat gatcacgccg
     6181 gtgttggggt cgccctcggc ggtataggca taggcgttgt cggacagctt ctcccagctg
     6241 atcttcttct cttcgaggtc ggcctgggag gcgaatttct tggtgctcat gaggggaggt
     6301 ctccggtgat gtgcagcgat ggatccatac tagttgtgag ttcgttaata gtcaatacgt
     6361 ttgttatagg caaatcttcc ggatacgcgg ccagtccggt ggactgttaa gatgcgccgc
     6421 gaatccggag agcagcatgc aggacatccc taatccctcc ccctcgaccg cacgcgatgc
     6481 ggccgagtcc gccgccgagc gccgcggcat ccagtccatc gaggtcggcg gcagcctgct
     6541 gtccgccctg gtgcgcgaag gcaagccgat gatgctgaag gacctcgcac gcgaggccgg
     6601 catgccggcg gccaaggcgc atccctacct ggtgagcttc ggcaagctcg gcctggtgga
     6661 gcaggacgcg ctcaccggcc gctacgggct cggcccgttc gcgctgcaga tgggcctgag
     6721 cgcgctgcac ggcctcgatc cgatcaaggc ggccctgccc gaagccgccc ggctcgccga
     6781 cgacatcgag ctcaacgtgg cgatcgcggt gtggggcaac cacggcccca ccgtcgtgca
     6841 catcgaggag tgcagccgcc agatccacat caacatgcgg cccggcacgg tcatgacgcc
     6901 cttgctgctg tcggccaccg ggcgcgtgtt cgcggccttc ctgccgcagc gcatcgtcgc
     6961 ccccgtgctc gccgccgaag cggcggcggt gggcgcccca ccgctggaga tgtcccccgc
     7021 cgacgtccgg gcggtgctcg acgagatccg cacgcagcgc ctcgcgcgcg cactcggcaa
     7081 tccgatcccg ggcatccatg cgttttccgc gccggtcttc gacggcggcg gccagctcgc
     7141 gctcgcgatc accgcgatgg gaccggtcgg cagcgtcgat ccggactggg aaggcctcac
     7201 cgcatgccgg gtgcgcgcgg cagcgaaggc gatcgagcgc cgcctcggca agcaggcggc
     7261 ctgagccacg tcaaggcggc cgacaccggc gcggacggag aatcggcgct ccccaacgcc
     7321 ccggagcgag catgtccagc gaacgttttc ccgctttctt cgaccaggtg ccacgcctcg
     7381 tggtgcgcga tccgctcgcc gagttcctcg gcgccgccga ggggggcgtg ctcgagtacg
     7441 gctacgccga cgcggtgcgc ctggccggcc attcctgccc gacggtggcc tcggcctacg
     7501 ccctcgccca ccacgcgatg acgcggctct tccccggcgc attgcccgtg cgcggcttgg
     7561 tgcaggtggg cttccagcat cctctcgacg agggcgtgac cggcgtcgtc gccgcggtgg
     7621 tcgggctcct caccggcagc gcccaggacg gcggcttcaa gggcatcgcc gggcgcttcg
     7681 tgcgccgcaa cctgcagcgc ttcggcgaac cgcagccgct cgcgctgcgc ttcagccgca
     7741 gcgacaccgg cgcctccgtg gacgctgccg ccgaactggc aaaggtgcag ccccaccccg
     7801 agatggccga gcgcatgggc cgctgcatgc gcggcgacgc cgacgccggc gaactgcagc
     7861 gcttcggaca gctgtggcag gaccgcgtcc ggcgcgtgct cgtcgagcac tgggacgacc
     7921 ccgaggtgtt ccacttttcc ggctgatcgg ccggcttccg ccgcccagcc cgtcccatag
     7981 cagcttggcg ccgcacgacc gggcgaaggg cagcacgcgc ggtccttcgc cggtcgcggc
     8041 gaccgcggtg cgccggcgca gcaggcggtc ggcaacgaag gcgagcacga tcagcccgag
     8101 cacgagcttg accatgcggt cggagagcac gccgaagagc agcgtccccg cgccgatgcc
     8161 gagcagcgcg gccgggatca ggtggcgcag atcgccccgg tcggccttgc cgcgccacac
     8221 ccggataccg accatgtcca tcgcgctcag gatcggcaac accaccgcca ctgcgtcgcg
     8281 cggcgggatc cacatcgaca tgaagggcac cgccagcccg ccgagcgccc cgcccagcct
     8341 tggagatgcc ggtgagcagg atggcgaggc ggtgacgaac cagccgaggg cgtccatcgc
     8401 cgggcaggcg cgggggcgat cagctcaggc ggagggcgat gtattgcagc gcgagaccgt
     8461 agccgtgcac gccgaagccg gccatgaccg cctcggcgac gtccgacagg taggagtggt
     8521 ggcggaaggg ctcgcgcttg tgcacgttgg aaatatgcac ctcgacgaag gggatcgcca
     8581 ccccggcgag cgcgtcgcgg atcgccacgc tggtatgggt gtaggcaccc ggattgatga
     8641 ggatccacgc gacgccctcg gtgcgcgcag cgtggatgcg atcgacgatc gcgccctcgt
     8701 ggttgctctg gaagcacagc agctcgatgc cgagcgcctc gccctgcgcg cgcaggccgt
     8761 tctcgacgtc ggcaagcgtg gtcgcgccgt agatctcggg ctcgcgggtg ccgagcaggt
     8821 tgaggttggg gccgttgatg acgaggacct tgggcatggg gggcgcgctc cgggcggcgg
     8881 gtagaaaaaa aggcggtccg cggaccgcct ttcgcaaatc gcttcagcgc agaggatatc
     8941 agcgcggcag caggctctca cccatcagga actcgtcgac cgcacgggca cactggcggc
     9001 cctcgcgcct tgcgggccgg tcggcccgca atcgtcaact ctcccctcgt gggcgattcg
     9061 cctatcgggg caacaagctc tcacccatca ggaactcatc gaccgcacgg gcgcactggc
     9121 ggccctcacg aatcgcccac accaccagcg actgcccgcg gcgcatgtcg ccggcggcga
     9181 agaccttggg cacgttggtg gtgtagctgc ccgcaccgtc ggtgctcgcc ttggcattgc
     9241 cgcgggcgtc cttgtccaca ccgaaggctt cgagcacgcc gcccaccggc tgggtgaagc
     9301 ccatcgcgaa cagcaccagg tcggccttga tctcgaactc ggagcccggc acctcggaca
     9361 tcttgccgtc cttgaactcc aggcgacagg ccttgagcgc cttgaccttg ccgttcttgc
     9421 cgatgaactc cttggtggcg acggcgaagt cgcgctcgca accttcctcg tgcgaggacg
     9481 aggtgcgcag cttggtcggc cagtagggcc aggtcagcgc cttgttctcc tgctcgggcg
     9541 gcatcggcat gagctcgaac tgggtcacgc tggctgcgcc gtggcggttg gaggtgccca
     9601 cgcagtcgga gccggtgtcg ccaccgccga tcaccaccac gtgcttgccc ttggccgaga
     9661 tcggattggg cttgtcgccg gcgacctgct tgttctgcgg gatgaggaac tcgagcgcgt
     9721 aatgcacgcc gccgagctcg cggcccggca ccggcaggtc gcgcgggatc tcggagccgc
     9781 cggcgagcac cacggcgtcg aagtcctgct tgagctgctc ggcgctgacg acctgcttcg
     9841 catcattgac gatgccggcg ggcacattct gcgcgccgac cagcacgccg gtgcgaaaca
     9901 ccacgccctc ggcctggagc tgctcgacac ggcggtcgat cagcgacttc tccatcttga
     9961 agtcggggat gccgtagcgc agcaggccgc cgatgcggtc gttcttctcg aacacggtga
    10021 cgtcgtggcc ggcgcgcgcg agctgctgcg cggccgcgag gccggccggg ccggagccga
    10081 ccacggcgac cttcttgccc gtcttgacct cggccggctg cggcaccacc cagccctctt
    10141 cccaggcctt gtcgatgatc gcgtgctcga tcgacttgat gcccaccgcg tcggtgttga
    10201 tgttcagcgt gcacgccgcc tcgcagggtg cggggcagat gcggccggtg aactcgggaa
    10261 agttgttggt cgagtgcagc acctcgatcg cctcgcgcca gtggccgcga tacacgaggt
    10321 cgttccagtc cgggatgatg ttgttgaccg ggcagccgtt gttgcagaac gggatgccgc
    10381 agtccatgca gcgcgcgccc tggaccgacg cctgctcgtc ggtgaggcgg acgacgaact
    10441 ccttgtaggt cttcaggcgc ttgtcgaccg gctcgtaggc ctcggacaga cgctgatatt
    10501 ccatgaatcc ggtgggcttg cccatttatg cggcctcctt ctcagcttcg cgctgctgag
    10561 ccatctcggt cagcgcgcgg cggtactcgt gcgggaacac cttgacgaac ttcttccgcc
    10621 agaggcccca gttgttcagg atctcgcgcg cacgcacgct gctggcgaag cggacgtggc
    10681 gctcgatcag gcccttgagg atgagctcgt cgcccatcgt caggtggtcg atgtcgacgc
    10741 ggccgtggga ctcgagctcg tcgcccgact ccgagccctt gcgcgcggcg atctcctcgg
    10801 gcagcggctc gagcgcgacc tgcgccatgt tgcagcgctg ctcgaacgtg ccatcctcgt
    10861 cgagcacgta ggccacgccg cccgacatgc cggcggcgaa gttgcgcccg gtctcgccga
    10921 gcacgaccac ggtgccgccg gtcatgtact cgcagccgtg gtcgcccacg ccctccacca
    10981 ccgcggtggc gccggagttg cgcaccgcga agcgctcgcc cgcgacgccg gcgaagtaga
    11041 cctcgccctc ggtcgcgccg tagagcacgg tgttgccgac gatgatgttc tgcggcgtgt
    11101 cgccgcggaa ctcggccttc gggcgcacga tgatgcggcc gcccgaaagg cccttgccga
    11161 cgtagtcgtt gccctcgccc accagctcga gcgtgacgcc gcgcgccagg aaggcgccga
    11221 agctctgccc ggcggtgccg ttgaggcgga tgtggatggt gtcgttgggc aggccggcat
    11281 ggccgtagcg cttggccacc tcgcccgaga gcatcgcgcc cacggtgcgg ttgatgttgc
    11341 gcaccggcag gtcgatgttg accttctcac ccttctccag tgccggcttg gcgagcacga
    11401 cgagctgcct gtcgagtgcc ttgtcgaggc cgtggtcctg cgtctcgcga tgcaggcgcg
    11461 gcacctcggc gggcacgttg ggcaggtaga agatgcgcga gtagtcgagg ccctgcgcct
    11521 tccagtgcgc gatgcccttc ttcatgtcga gcaggtcggc acggccgatg agctcgtcga
    11581 aggtccgcac gccgagctgc gccatcagct cgcgcacctc ctcggcgacg aagaagaagt
    11641 agttgaccac gtgctcgggc tggcccgaga agcgcgcgcg cagcaccggg tcctgggtgg
    11701 cgacgccgac cgggcaggtg ttgaggtggc acttgcgcat catgatgcag cc
//
LOCUS       DAOQBS010000102        11497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4800_length_11497_cov_16.970460, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000102 DAOQBS010000000
VERSION     DAOQBS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11497
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4800_length_11497_cov_16.970460"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="PKN25_11335"
     CDS             complement(<1..1238)
                     /locus_tag="PKN25_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV91535.1"
                     /translation="MSSPIAASSSLPAQLPLGLRDVAALGQVFTPEPVVRAMVALRRN
                     AGRVLEPSCGDGAFLRHLPGAVGLELDPDHCPPGAQAIDFFAYPERERFDAIIGNPPY
                     VRIQDIAESTRALIERGAYGDLLDGRANLYLFFIAKCLRHLRPGGELIFITPRDFLKA
                     TSAVKLNRLLVESGSITDAIELGDARVFDDAVPNCLIWRFEKGRSARAMRYCALGVGD
                     DLAAGLAAPAWEERHLVEAGGHLMFARGDYPLRLADVAFVKVGAVSGADELFTDAVNG
                     NRDFVCSSTVSTGQTRRMIWSEPGDPPPALLAPHKARLLQRKVTRFDESNWWMWGRLH
                     HRSAQPRVYVNGKTRVPKPFFVHPCTDYDGAVLAIFPRRADVDIEAFRDALNAVDWED
                     LGFVCDGRFLFTQRSLEHAPL"
     assembly_gap    1277..1376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1377..3434)
                     /locus_tag="PKN25_11340"
     CDS             complement(<1377..3434)
                     /locus_tag="PKN25_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like protein"
                     /protein_id="HNV91536.1"
                     /translation="MKEKELRLALVLFGGVSLAVYQHGINRELLNLARASRAYHRVEG
                     PAAKQAPGHVYPAGAGADAWTAEVYFDLLKRLGRMVDLRVLVDVISGASAGAINGIAL
                     ARALAHDLSLAPVTRLWLERADMQRLIAPEARAGKWDKWYFRPLLRPLLAWARREGML
                     ETQPDPETLERALAFVRSRWFSAPLDGALLSAELLDGLLAMETGSVAAGSLLPSGTCL
                     SLAVTVTDFRGIERALFTHDPPLLREREHRHLLRFACEHRRTGELDSDFGLDNAPSLA
                     FAARASASYPGAFPPARLAEMDALLAARGLTWSTRERFLARNFAHYRASGMNPAEVVL
                     LDGSVLDNKPILAAVGYIRVHRAFREVDRRLIFIDPHAEARVGQGAGSDGGEGEPGWF
                     EVLRSALSDLPRHQPIHQELAEISRYNRQIRRLKATIVQSRPEVEALVERATGGALSG
                     PFTAVELRHWRLTSTNMLGAMPLVYDPWWRALVLEALDFLAGLLGSLCGCARESPGAR
                     WLQQVVEAWAAHAGILREHYRIADDVGEDADMPAFARVVIRFGIEYKRRRINFVLHEL
                     NTLYHALPGEGRCTTAPGVLDVVKTRIHECLDALAIYDDCAFVDAPAAAAVRALLQPA
                     TVAAAAQPEAEAVAFATRNALALDALIERIGDACRIGEANANMDAVLVSDAVRAIEPH
                     CRRQ"
     gene            3596..4573
                     /gene="cysK"
                     /locus_tag="PKN25_11345"
     CDS             3596..4573
                     /gene="cysK"
                     /locus_tag="PKN25_11345"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNV91537.1"
                     /translation="MSTWYPDNAESIGRTPLVRLNRVLDGAQATVLAKIEGRNPAYSV
                     KCRIGAAMVKDAIAHGRLGPGQEIVEPTSGNTGIALAFVCAARGIPLTLTMPETMSVE
                     RRKLLVAYGARLVLTEGPKGMNGAIAKAKEIVDSDPGRYVLLQQFENPANPAIHETTT
                     GPEIWNDTDGGIDILVSGVGTGGTITGISRYIKRVRGKDIRSVAVEPAASPVITQTLA
                     GQPLTPAPHKIQGLGAGFVPKVLDLSLIDAVEQVSNEDAVLYARRLAREEGILAGISC
                     GAAVAAAARVAKQPENAGKTIVVILPDSGERYLSSILFEGLFDEKGMAI"
     gene            4581..4961
                     /locus_tag="PKN25_11350"
     CDS             4581..4961
                     /locus_tag="PKN25_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNV91538.1"
                     /translation="MTIRVRRAYDPPAAGDGCRVLVDRLWPRGLARENARIEHWLREL
                     APSSELRKWFAHDPARWDEFRRRYADELRAPAAAAALAELHGLIAAHPVLTLLHAARD
                     TTHNNAEALRLWLACGAASRPGNA"
     gene            5141..6187
                     /locus_tag="PKN25_11355"
     CDS             5141..6187
                     /locus_tag="PKN25_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNV91539.1"
                     /translation="MFRLPWPTRKDASTPVALALQGGGAHGAYTWGVLDRLLEAGVPI
                     EGISGTSAGAMNAVALAEGWTTGGAEGARAALDRFWTAVGDSVPFHLELLQSLSPSGD
                     GSLPSPMNMMLGLARVFSPYQLNPFELNPLRDVVRAQFDFERIRRACPLKLFIAATAV
                     RTGKVRLFRTAELGEAAVLASACLPTLHHAVEIDGEHYWDGGFTANPAIYPLLYECDT
                     PDLLMVLLNPLQHEQAPRSAEDIAARSMELGFSTTFLREMRMIAHARQFIAERPRWSP
                     IGRLERKLLRERFHLIDAPELGDAGSASKLDATASALLRLRELGRARAETWLQAHAHE
                     VGRRETIDVGALFL"
     gene            complement(6245..8236)
                     /locus_tag="PKN25_11360"
     CDS             complement(6245..8236)
                     /locus_tag="PKN25_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HNV91540.1"
                     /translation="MARKTHPMVASLRAADTGKIVSAREAVRLIRSGDTVATGGFVGI
                     GFAESVAIALEQRFLEAEGGPGSPRDLTLVYAAGQGDGKARGLNHLGHEGLVARVIGG
                     HWGLVPRLQALAVDNRIEAWNLPQGVITHLFRDIAAGKPGTLTRVGLGTFVDPRFGGG
                     KLNARTTEDLVRLMPIDGEDYLFYKAFPIHVGIIRGTTADPDGNVTMEKEALTLEARA
                     IAMAARNSGGIVIVQVERIAERGSLNPRQVQVPGVLVDCVVVADRPEHHMQTYSEQYN
                     PAFSGEIRVPMSAICAMPMSARKIIARRAAMELQSAAVVNLGIGMPEGVATVAAEEKL
                     IDLLTLTAEPGVIGGIPAGGLSFGAAVNTQAIIDQPSQFDFYDGGGLDIAFLGLAQAD
                     REGNLNVSKFGPRLAGAGGFINISQNAKKVVFVGTFSAGGLEVAVEDGCLRIVREGET
                     RKFVDAVEHRTFSGTQAAKWRQDVLYVTERCVFRLCEDGLELIEIAPGIDLQRDILAR
                     MDFVPVMKGPPVRMDARIFADAPMGLRHDLLDVPLGRRLAYDAQQDVFFVDFEGLSVR
                     TPQDIAAIRDAVRAALAPLGRKVDAVVNYDRFSIVPELVDDYVEMVKGLMDAHYHTVT
                     RYTANGFLRMKLGEELEKRRIPAHFYASASEAWHGLETP"
     gene            complement(8383..10620)
                     /locus_tag="PKN25_11365"
     CDS             complement(8383..10620)
                     /locus_tag="PKN25_11365"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNV91541.1"
                     /translation="MSPPIETLPGPNSEPRSKTSSPRPPAADPLAALDPAQRAAVGHG
                     IGADGRIDAGPLLVIAGAGSGKTGTLAHRVARLVAAGADPARILLLTFSRRAADEMGR
                     RVRRILAQAAADRPGLARAELQWAGTFHAIGARLLRDHAGRIGLDPGFSIHDREDSAD
                     LMNLVRHELGLSATKQRFPQKGTCLAIYSAVVNTRAALAEVLQASFPWCAQWEDELKR
                     LFRAYVEAKQAQQVLDYDDLLLYWAQMVGEAELGAEIGGLFDHVLVDEYQDTNALQAA
                     ILLAMKPDGRGLTVVGDDAQSIYAFRGATVRNILDFPAAFAPPARVVTLERNYRSTQP
                     ILDAANAVIALAAERFAKNLWSERASAQRPRLVTVKDEAAQADFVVDSVLARREEGLK
                     LKQQAVLFRASAHSARLELELTRRNIPFVKYGGLRFLEAAHVKDVLALLRWAENPRDR
                     IAGFRAIQLLAGIGPRTAGRVLDNVCSAPPGCVLLAEQPVPEAARPGWLEFATLVDRL
                     SGREEASTAAFAPGGAPARTTAPVRAEGLAGGARGGLPWPTDFELACRWYEAQMPRLY
                     DDFQVRVLDIQQLARIAATSPSRQRFLTELTLDPPSATSAEAGMPLLDEDYLILSTIH
                     SAKGQEWNAVSVLNVVDGCIPSDVATRDSAEIEEERRLLYVAMTRAKDALDLVVPQRF
                     HVTQQTGLGDRHVYAGRSRFIPNRLLGHFEQMSWPLPAPELQGSPASRQAAIDLAAKM
                     RDMWR"
     gene            10719..11354
                     /locus_tag="PKN25_11370"
     CDS             10719..11354
                     /locus_tag="PKN25_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91542.1"
                     /translation="MPAPEPLLPSCLQPRRVILFSGHMIDAPGRAEPRFPPALAPAAA
                     ARIAEALDRLEAGPLDLGLTQGAAGGDLLFAEAAQARALPLLFLQPFGEDEFIARSVR
                     PVSAGEQWVARYRAVAARLASPPHAMPPAPAAAADNPFERCNRWLLDTALACGGERVW
                     LLCLWDGRRGDGAGGTAHMVEEVSRHRGHVLHIDTRELRPHDPAGSPPLPN"
BASE COUNT         1675 a         4154 c         4039 g         1529 t
ORIGIN      
        1 agcggcgcgt gctccaggct gcgctgggtg aacaggaagc ggccgtcgca gacgaagccg
       61 aggtcctccc agtccaccgc gttgagggcg tcgcggaagg cctcgatgtc cacgtcggcg
      121 cggcgcggga agattgccag cacggcgccg tcgtagtcgg tgcagggatg gacgaagaag
      181 ggcttcggca cccgcgtctt gccgttcaca tacacccgcg gctgcgcgct gcggtggtgc
      241 aggcggcccc acatccacca gttcgactcg tcgaagcggg tgaccttgcg ctgcagcagg
      301 cgcgccttgt gcggcgccag cagcgccggc ggcggatcgc ccggctcgct ccagatcatg
      361 cgccgggtct gcccggtgct gaccgtcgac gagcacacga agtcgcggtt gccatttacc
      421 gcgtcggtga acagctcgtc cgcgcccgac accgcgccga ccttgacgaa ggccacgtcg
      481 gccaggcgca gcgggtagtc gccgcgcgcg aacatcaggt ggccgccggc ctcgaccaag
      541 tggcgctcct cccaggccgg cgccgccagc ccggcggcga ggtcgtcacc gacgccgagc
      601 gcgcagtagc gcatcgcgcg cgcgctgcgc cccttctcga agcgccagat caggcagttc
      661 ggcaccgcgt cgtcgaacac gcgcgcgtcg ccgagctcga tcgcgtcggt gatcgagccg
      721 gactcgacca gcaggcggtt gagcttcacc gccgaggtgg ccttcaggaa gtcgcgcggg
      781 gtgatgaaga tcagctcgcc gcccgggcgc aggtggcgca ggcacttggc gatgaagaag
      841 aggtagaggt tggcgcggcc atcgaggagg tccccgtagg cgccgcgctc gatcagtgcc
      901 cgcgtgctct cggcgatgtc ctggatgcgc acgtagggcg ggttgccgat gatggcgtcg
      961 aagcgctcgc gctcggggta ggcgaagaag tcgatcgcct gcgcgccggg cgggcagtgg
     1021 tcggggtcga gctcgagacc gaccgcgccc ggcaggtggc gcaggaaggc gccgtcgccg
     1081 cacgagggct cgagcacgcg gcccgcgttg cgacgcaggg cgaccatggc gcgcaccacc
     1141 ggctcggggg tgaacacctg gccgagcgcg gcgacgtcgc gcaggcccag gggaagctgg
     1201 gcgggcaggg aggaagaggc ggcgatcgga gaggacatgg ggcgcgatct taccaagcgc
     1261 gcgcgcggcg agggccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgg
     1381 cggcggcagt gcggctcgat cgcccgcacc gcgtcggaga ccagcaccgc gtccatgttc
     1441 gcgttggcct cgccgatccg gcaggcgtcg ccgatgcgct cgatcaaggc gtcgagcgcg
     1501 agcgcgttgc gcgtcgcgaa cgccaccgcc tccgcctcgg gctgcgcagc ggcggcgacg
     1561 gtcgccggct gcagcagggc acgcaccgca gcggcggcgg gtgcatcgac gaaggcgcag
     1621 tcgtcgtaga tcgccaatgc gtcgaggcac tcgtggatgc gcgtcttcac cacgtcgagc
     1681 acgcccgggg cggtggtgca gcggccctcg cccggcaggg cgtggtagag ggtgttgagc
     1741 tcgtgcagca cgaagttgat gcggcggcgc ttgtactcga tgccgaaacg gatcacgacg
     1801 cgcgcgaacg cgggcatgtc ggcatcctcg cccacatcgt ccgcgatgcg gtagtgctcg
     1861 cgcaggatcc cggcgtgcgc cgcccacgcc tccaccacct gctgcagcca gcgcgcgccc
     1921 ggcgattcgc gtgcgcagcc gcacaggctg ccgagcaggc cggcgaggaa atcgagcgct
     1981 tccagcacca gcgcacgcca ccacgggtcg tacaccagcg gcatcgcccc cagcatgttg
     2041 gtcgaggtca accgccagtg gcgcaactcg acggccgtga agggccccga cagggcacca
     2101 ccggtggcgc gctcgacgag cgcctcgacc tcgggccggc tctgcacgat ggtcgccttg
     2161 aggcggcgga tctggcggtt gtagcggctg atctcggcaa gctcctggtg gatcggctgg
     2221 tggcgcggca ggtcggacag ggcactgcgc aggacctcga accagcccgg ctcgccctcc
     2281 ccgccatcgc tgccggcgcc ctggccgacg cgcgcctcgg catgcggatc gatgaagatc
     2341 agccgccggt ccacctcgcg gaaggcgcga tggacccgga tgtagccgac cgcggcgagg
     2401 atgggcttgt tgtcgagcac gctgccgtcg agcaacacca cttcggcggg gttcatgccg
     2461 ctcgcgcggt agtgcgcgaa gttgcgcgcc aggaagcgct cgcgcgtact ccacgtcagc
     2521 ccgcgcgcgg ccagcagggc gtccatctcg gccagccggg cgggcgggaa ggcgcccgga
     2581 taggacgccg aggcgcgcgc cgcgaaggcg agcgagggcg cgttgtcgag accgaagtcg
     2641 ctgtcgagct cgccggtcct gcgatgctcg caggcgaagc gcagcaggtg gcgatgctcg
     2701 cgctcgcgca gcagcggcgg gtcgtgggtg aacagcgcgc gctcgatgcc gcggaagtcg
     2761 gtcaccgtga ccgccagcga caggcaggta cccgagggca gcagcgagcc ggcggccacc
     2821 gaacccgtct ccatcgcgag caggccgtcg agcagctccg cgctcagcaa ggccccgtcg
     2881 agcggcgcgc tgaaccagcg cgaacgcacg aaggcgagcg cgcgctcgag cgtctcgggg
     2941 tcgggctggg tctcgagcat gccctcgcgc cgggcccacg ccagcagcgg ccgcagcagc
     3001 gggcggaagt accacttgtc ccacttcccg gcgcgcgcct ccggtgcgat caggcgctgc
     3061 atgtcggcgc gctccagcca cagccgcgtc accggcgcca ggctgagatc gtgcgcgagc
     3121 gcgcgcgcga gcgcgatgcc gttgatggcc cccgccgacg cgcccgagat cacgtcgacc
     3181 agcacgcgca ggtccaccat gcgcccgagg cgcttgagca ggtcgaaata cacctcggcg
     3241 gtccacgcgt cggcgcccgc gcccgccgga tacacatggc cgggcgcctg cttggcggcg
     3301 ggcccttcga cgcggtggta ggcgcgcgag gcgcgcgcga ggttgagcag ttcgcggttg
     3361 atgccgtgct ggtacaccgc gagcgaaacg ccgccgaaca gcaccagtgc gagccgcagt
     3421 tccttttcct tcattgcccc ctcccgcgtg ccggacctcg cgcaagggct tcaagcttgc
     3481 acctgcgcgc gccgacgtca agcgccgtcc gccaccacgc gcgacgatcc ggcgcgaccg
     3541 tgcggcgcgc tttgcctata cttccggagc acccccaacg aggagaagag aaccgatgtc
     3601 cacctggtac cccgacaatg cagaatccat cggccgcacc ccgctcgtgc gcctgaaccg
     3661 cgtgctcgac ggcgcgcagg ccaccgtgct ggcgaagatc gagggccgca acccggccta
     3721 ctcggtcaag tgccgcatcg gcgcggcgat ggtcaaggac gcgatcgccc acggccgcct
     3781 cggccccggc caggagatcg tcgagcccac cagcggcaac accggcatcg cgctcgcctt
     3841 cgtgtgcgcc gcgcgcggca tcccgctgac cctgaccatg cccgagacca tgagcgtgga
     3901 gcgccgcaag ctgctcgtcg cctatggcgc cagactggtg ctgaccgaag gccccaaggg
     3961 catgaacggc gccatcgcca aggccaagga gatcgtcgac agcgaccccg gccgctacgt
     4021 gctgctgcag cagttcgaga accccgccaa cccggcgatc cacgagacca ccaccggccc
     4081 ggagatctgg aacgacaccg acggcggcat cgacatcctg gtgtcgggcg tgggcaccgg
     4141 cggcaccatc accggcatct cgcgctacat caagcgcgtg cgcggcaagg acatccgctc
     4201 ggtcgcggtc gagcccgcgg ccagcccggt catcacccag accctcgccg gccagccgct
     4261 cacgcccgcg ccgcacaaga tccagggcct gggcgcgggc ttcgtgccga aagtgctcga
     4321 cctctcgctg atcgacgcgg tcgagcaggt gagcaacgaa gacgcggtgc tctacgcccg
     4381 ccgcctggcg cgcgaggagg gcatcctcgc cggcatctcc tgcggcgcgg cggtggccgc
     4441 cgcggcgcgc gtcgcgaagc agcccgagaa cgccggcaag accatcgtgg tgatcctgcc
     4501 cgactcgggc gagcgctacc tgagctcgat cctattcgag ggcctgttcg acgagaaggg
     4561 catggcgatc tgaccagacc atgacgatcc gcgtgcgccg cgcgtacgac ccacccgcag
     4621 ccggggacgg ctgccgggtg ctggtcgatc gcctgtggcc gcgcggactc gcgcgcgaga
     4681 acgcacggat cgagcactgg ctgcgcgagc tcgcgccctc gagcgagctg cgcaagtggt
     4741 tcgcccacga cccggcgcgc tgggacgaat tccgccgccg ctacgccgac gagctgcgcg
     4801 cccccgcggc cgccgcggca ctcgccgagt tgcacggcct gatcgccgcg catcccgtcc
     4861 tcaccctgct ccacgccgcg cgcgacacca cgcacaacaa cgccgaggcg ctgcgcctgt
     4921 ggctggcgtg cggagccgct tcccgacccg gcaatgcctg acccggcggc gggccggcgc
     4981 gcacgacgcc cgcgcccgcg catcctgtta ccggtacgca cggctgtaat ccggactgcg
     5041 tgataatctg cattgtgcag tgcacaatgc agacctgcag cttgccggac aggcgccaag
     5101 ggactcccct gccgtccgcc cggacccgaa gggggtttcg atgtttcgct tgccgtggcc
     5161 aaccaggaag gatgcctcca cccccgtcgc gctcgcgctg cagggcggtg gcgcgcatgg
     5221 cgcgtacacc tggggcgtgc tcgaccgcct gctcgaggcg ggagtgccga tcgaaggcat
     5281 cagcggcacc agcgccggcg cgatgaatgc cgtggcgctc gccgagggct ggaccacggg
     5341 cggggcggag ggcgcgcgcg ccgcgctcga ccgcttctgg acggccgtcg gcgacagcgt
     5401 tcccttccac ctcgagctgc tgcaaagcct cagcccctcg ggtgacggca gcctcccctc
     5461 gccgatgaac atgatgctcg ggctcgcccg cgtgttctcg ccctatcagc tcaacccctt
     5521 cgagctcaat ccgttgcgcg acgtggtgcg cgcgcagttc gacttcgagc gcatccgccg
     5581 cgcgtgcccg ctcaagctct tcatcgccgc caccgcggtg cgcaccggca aggtcaggct
     5641 gttccgcacc gccgagctcg gcgaagcggc cgtgctggcc tcggcctgcc tgcccacgct
     5701 gcatcacgcg gtggagatcg acggcgagca ctactgggac ggcggtttca ccgccaaccc
     5761 ggcgatctac ccgctgctct acgaatgcga cacgccggac ctgctgatgg tgctgctgaa
     5821 ccctctgcag cacgagcagg ccccccgcag cgccgaagac atcgcggcgc gcagcatgga
     5881 gctcggcttc tccaccacct tcctgcgcga gatgcgcatg atcgcccacg cccgccagtt
     5941 catcgccgag cgtccgcgct ggtcgccgat cggccgcctc gagcgcaagc tgctgcgcga
     6001 gcgcttccac ctgatcgatg cccccgagct cggcgacgcc ggcagcgcga gcaagctcga
     6061 tgccaccgcc agcgccctgc tccggctgcg cgaactcggc cgcgcacgcg ccgaaacctg
     6121 gctgcaggcg catgcccacg aggtcggccg gcgcgagacg atcgatgtcg gcgccctgtt
     6181 cctgtgaggc ctgcatgccc gacaccggcc ccgtgcgggc gcagcgcggc ggcgcagcgc
     6241 cccgctacgg ggtttccagc ccgtgccagg cttcgctcgc actcgcgtag aagtgcgcgg
     6301 ggatgcgccg cttctcgagc tcctcgccga gcttcatgcg caggaagccg ttggcggtgt
     6361 agcgggtcac ggtgtggtaa tgggcatcca tcagcccctt caccatctcg acgtagtcgt
     6421 ccaccagctc cggcacgatc gagaagcggt cgtagttgac caccgcatcc accttgcgcc
     6481 cgagtggagc gagcgcggcg cgcaccgcgt cgcgaatcgc ggcgatgtcc tgcggggtgc
     6541 gcaccgacag cccctcgaag tcgacgaaga acacgtcctg ctgcgcgtcg taggccagcc
     6601 gacgcccgag cggaacgtcg agcaggtcgt ggcgcaagcc catcggcgcg tcggcgaaga
     6661 tgcgcgcgtc catccgcacg ggcgggccct tcatcaccgg tacgaagtcc atgcgagcga
     6721 ggatgtcgcg ctgcagatcg atccccggcg cgatctcgat gagctcgagt ccgtcctcgc
     6781 acagccggaa cacgcagcgc tcggtgacgt agagcacgtc ctgccgccac ttcgccgcct
     6841 gcgtcccgct gaaggtgcga tgctcgaccg cgtcgacgaa cttcctcgtc tcgccctcgc
     6901 ggacgatgcg caggcatcca tcctccaccg ccacctccag cccgcccgcg ctgaaggtgc
     6961 cgacgaagac gaccttcttg gcattctgcg agatgttgat gaagccgccc gcgcccgcga
     7021 gccgcgggcc gaacttcgac acgttgaggt tgccctcgcg gtcggcctgg gcgaggccga
     7081 ggaaggcgat gtccagcccg ccgccgtcgt agaagtcgaa ctggctgggc tggtcgatga
     7141 tggcctgggt gttcaccgcc gcgccgaagc tcagcccgcc ggcgggaatg ccgccgatca
     7201 cgcccggctc ggcggtgagc gtgagcaggt cgatgagctt ctcctccgcg gccacggtcg
     7261 cgaccccctc cggcatgccg atgccgaggt tcaccaccgc agccgactgc agctccatcg
     7321 cggcccggcg ggcgatgatc ttgcgcgcgc tcatcggcat cgcgcagatc gccgacatcg
     7381 gcacgcggat ctcgccggag aaggccgggt tgtactgctc actgtaggtc tgcatgtggt
     7441 gctcgggacg atccgccacc accacgcagt cgaccagcac gcccggcacc tgcacctggc
     7501 gcggattgag cgagccgcgc tcggcgatgc gctcgacctg cacgatgacg ataccgccgg
     7561 agttgcgcgc ggccatggcg atcgcgcgcg cctccagggt cagcgcctcc ttctccatgg
     7621 tgacgttgcc gtcggggtcg gcagtggtgc cgcggatgat gccgacgtgg atcggaaagg
     7681 ccttgtagaa caggtagtcc tcgccgtcga tcggcatcag ccgcacaaga tcctcggtgg
     7741 tgcgcgcgtt gagcttgcca ccgccgaagc gcggatcgac gaaggtgccc agcccgaccc
     7801 gggtcagcgt gcccggcttg ccggcggcga tgtcgcggaa caggtgcgtg atcaccccct
     7861 gcggcaggtt ccaggcctcg atgcggttgt ccaccgcaag cgcctgcagg cgcggcacca
     7921 gcccccagtg gccgccgatc acgcgcgcca ccaggccctc gtggccgagg tggttgaggc
     7981 cgcgcgcctt gccgtcgccc tgcccggccg catacaccag ggtcaggtcg cgcgggctgc
     8041 ccgggccgcc ctccgcctcg aggaagcgtt gttcgagcgc gatcgcgacg ctctcggcaa
     8101 agccgatgcc gacgaagccg ccggtggcga ccgtgtcgcc gctgcggatc aggcgcaccg
     8161 cctcgcgcgc cgaaacgatc ttgccggtgt cggcggcgcg cagcgacgcc accatcggat
     8221 gcgttttcct tgccatggcg gatctccctc gcgccgcgcc gggaagcggc cggaccgtgt
     8281 tcggcgggtg atcaccacca ccagcgcgac gaacgaaaag actaccctat ccggagcgat
     8341 cgagcgccag ggcacgagcc ctccgcggtc gcgaccgcgc agtcatcgcc acatgtcgcg
     8401 catcttcgcg gcgaggtcga tcgccgcctg gcgcgaggcc ggcgagccct ggagttcggg
     8461 cgcaggcaac ggccagctca tctgctcgaa gtgcccgagc agacggttgg ggatgaagcg
     8521 gctgcgcccg gcatagacat ggcgatcgcc caggcccgtc tgctgggtga cgtggaagcg
     8581 ctgcggcacg acgaggtcga gcgcgtcctt ggcgcgcgtc atcgccacgt agagcaggcg
     8641 gcgctcctcc tcgatctcgg cgctgtcgcg cgtggcgacg tccgaaggga tgcagccgtc
     8701 gaccacgttg agcacgctca ccgcgttcca ctcctggccc ttggccgagt ggatggtcga
     8761 caggatcagg tagtcctcgt cgagcagcgg catgccggcc tcggcgctgg tggcgctggg
     8821 cggatcgagc gtgagctcgg tgagaaagcg ctgccggctc gggctggtgg cggcgatgcg
     8881 cgcgagctgc tggatgtcga gcacgcgcac ctggaaatcg tcgtacaggc gcggcatctg
     8941 cgcctcgtac caacggcagg cgagctcgaa gtcggtcggc cacggcaggc cgcccctcgc
     9001 cccgcccgca agaccctccg cacggacagg cgcggtggtt cgtgcaggag cgccgccagg
     9061 cgcgaatgcg gcggtcgagg cctcctcccg cccggaaagc cggtcgacca gcgtggcgaa
     9121 ctccagccac cccgggcgcg ccgcctccgg caccggctgc tcggcgagca gcacgcagcc
     9181 cggcggcgcg ctgcagacgt tgtcgagcac gcgcccggcg gtcctggggc cgatgccggc
     9241 gagcagctgg atggcacgga agccggcgat gcggtcgcgc gggttctccg cccagcgcag
     9301 gagcgcgagc acgtccttga cgtgggcggc ctcgaggaac ctgagcccgc cgtacttcac
     9361 gaaggggatg ttgcgccggg tcagctccag ttccaggcgt gcgctgtgcg ccgaggcgcg
     9421 gaacagcacc gcctgctgct tgagcttcag cccttcctcg cggcgcgcca gcacgctgtc
     9481 gaccacgaag tcggcctgcg ccgcctcgtc cttcaccgtc accaggcgcg ggcgctgcgc
     9541 cgaggcgcgc tcgctccaca ggttcttggc gaagcgctcg gcggcgagcg cgatcaccgc
     9601 gttggcggcg tcgaggatgg gctgggtgga gcggtagttg cgctccagcg tcaccacccg
     9661 cgccggcggc gcgaaggccg ccgggaagtc gaggatgttg cgcaccgtgg cgccgcggaa
     9721 ggcgtagatc gactgcgcgt cgtcgccgac cacggtcagg ccgcggccgt ccggcttcat
     9781 cgcgagcagg atcgccgcct gcagcgcgtt ggtgtcctgg tattcgtcga ccagcacgtg
     9841 gtcgaagagg ccgccgattt cggcaccgag ctccgcctcg cccaccatct gcgcccagta
     9901 cagcagcagg tcgtcgtagt cgagcacctg ctgcgcctgc ttggcctcga cgtaggcgcg
     9961 gaacaggcgc ttgagctcgt cctcccactg cgcgcaccac gggaagctcg cctgcagcac
    10021 ctcggcgagc gcggcgcgcg tgttgaccac cgccgaatag atcgccaggc aggtgccctt
    10081 ctgcgggaag cgctgcttcg tcgccgacag cccgagctcg tggcgcacca ggttcatcag
    10141 gtcggcggag tcctcgcggt cgtggatgct gaagccgggg tcgagcccga tgcggccggc
    10201 gtggtcgcgc agcaggcgcg cgccgatggc gtggaaggtg cccgcccact gcagctcggc
    10261 gcgcgccagg ccgggacggt cggcggcggc ctgcgcgagg atgcgacgca cgcgccggcc
    10321 catctcgtcc gcggcgcggc gcgagaaggt cagcagcagg atgcgcgcgg gatcggcccc
    10381 ggccgccacg aggcgcgcca cgcggtgcgc gagcgtgccg gtcttgcccg agccggcgcc
    10441 ggcgatcacc agcagcggcc cggcgtcgat gcggccatcg gcgccgatgc cgtgcccgac
    10501 cgcggcgcgc tgggcggggt cgagcgccgc gagcggatcg gcggccggcg gccgcgggga
    10561 ggaggtcttg gagcgaggct cggagttcgg cccgggaagg gtctcgatag ggggcgacat
    10621 ggctgtaagt atattcagcc tcggcgcgcg cagatagccg cggcagaggc gatcgctcca
    10681 gaatgtgcac agcaccgacc gaccaccgga gatccgcgat gcccgccccc gaaccacttc
    10741 tgccgagctg cctgcagcca cgccgcgtga tcctcttcag cggccacatg atcgatgcgc
    10801 ccggccgtgc cgaaccgcgc ttcccgcccg cactcgcgcc cgcggcggcg gcgcgcatcg
    10861 ccgaagcgct cgatcgcctg gaggccggcc cgctcgacct cggcctcacc cagggcgcgg
    10921 cgggcggcga cctgctcttc gccgaggcgg cgcaggcgcg cgcgctgccg ctgctcttcc
    10981 tgcagccctt cggcgaggac gagttcatcg cgcgctcggt acgcccggtg agcgcgggcg
    11041 agcagtgggt ggcgcgctac cgcgcggtgg ccgcccgcct cgccagcccg ccgcacgcca
    11101 tgccgcccgc ccccgcggcc gcggccgaca accccttcga gcgctgcaac cgctggctgc
    11161 tcgacaccgc gctcgcctgc ggtggcgaac gcgtctggct gctgtgcctg tgggacgggc
    11221 gccgcggcga cggcgccggg ggcaccgcgc atatggtcga ggaggtaagc cgccaccgcg
    11281 gccacgtcct ccacatcgac acccgcgagc tgcgaccgca cgatccggct ggcagccccc
    11341 cgctgccgaa ctaggctgaa tcgagccggg cgcggcaggc ccgcaatccg aaggtccagc
    11401 gcagacccct catgaccgcg acggcggcca cgcctgggag atcgcctgcg gcggatccgc
    11461 gacgcgatct gagcccccgc ccccgaccgg gggcgcg
//
LOCUS       DAOQBS010000103        11282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_4946_length_11282_cov_14.818117, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000103 DAOQBS010000000
VERSION     DAOQBS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11282
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4946_length_11282_cov_14.818117"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PKN25_11375"
     CDS             <1..686
                     /locus_tag="PKN25_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNV91543.1"
                     /translation="LLQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDDRVKDIANYIVK
                     GVLWQAFEAEQPTVVLIDEIDKADIEFPNDLLRELDRMEFHVYETRQTIQARHRPLVF
                     ITSNNEKELPDAFLRRCFFHYIRFPDRDTMQRIVDVHFPGLKKALLTEAMEVFFGLRD
                     IPGLKKKPSTSELIDWLKLLVAEDIPPEALRSADGKAAIPPLHGALLKNEQDVHLFER
                     LVFMARHNR"
     gene            703..2094
                     /locus_tag="PKN25_11380"
     CDS             703..2094
                     /locus_tag="PKN25_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV91544.1"
                     /translation="MLRALLGRLFGRKRPAPSVAAGEAEGFRRLQRAAPLRAEGAPAS
                     PVEAGATFLCREAVLGRNQRITGYQFMLQAGTRAHIRHSSRNIHHVFAEVLVRSLVDA
                     DIGELLGERLAFIDLPDSFLSHPSLAALPARNMVIVPTVLPDAGAPEPAALRAAVTAL
                     RARGMRIGLIDPTVVGELAHLLPQAEFIVAQAEGLDARRGLKLGNIALEVAHDARLVV
                     RGLPSLDDFRFCCKLGASLFQGPFITSREDWSARDPGPNTARIGALIARLRADADTRE
                     LADLLKQDAALSLRLLRYINSAANALPQPVSSIEHALALLGRERLQRWLILLACTADK
                     AGERGGAVLETALVRGRMMELLGAGRPAAESDALFLVGLLSLIDVILQVPLDKALAPL
                     ALGAEVEASVRGDHGPYQALLALTVACERGEAGEGAAGDALRAAAAACGIAPEQASEC
                     HLQALSWAMQIQD"
     gene            2117..3295
                     /locus_tag="PKN25_11385"
     CDS             2117..3295
                     /locus_tag="PKN25_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV91545.1"
                     /translation="MLIDFFLHLKARKLPVSTREFLTVLEGLRDHVCGPSLDDFYYYA
                     RLCLVKDESLYDRFDQAFGEYFKGVTALPGLEADLPEDWLRAMVKKHLSPEERAKLEK
                     LGWDKLMEEFRKRLQEQKGRHQGGSKWIGTGGSSPFGNNGTHPEGIRVGGESAGNRTA
                     VKVWDKREFRNLDDSVELGTRNIKVALRRLRRFARQGAAEELDLDGTIAATARNAGWL
                     DLMMRPERHNAVKVLLFLDVGGSMDDHVKVCEELFSACRLEFKHLEYFYFHNCVYEGV
                     WRDNRRRHAERTPLLDVIHKYGSDYKLIFVGDATMSPYEILHPHGSVEHMNAEAGATW
                     LRRLLDAYPAAAWLNPEPERLWPYRQSIGILGQIMGERMYPMTLDGLARAMQALSRRH
                     "
     gene            3326..4858
                     /locus_tag="PKN25_11390"
     CDS             3326..4858
                     /locus_tag="PKN25_11390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV91546.1"
                     /translation="MEETELPCPFCNAHSTRPIALPDGLWSACCADCGARGPIAPDAR
                     TALARWEQATRDGELLRTVIDESPDIILLKDWDGRFLLGNAALARLYDTTPEQLVGKD
                     DGAFNPNREQVDFYLENVRAVMRGGRTQIVEESSTNAETGEVHYFHSIKKPLRGPAGE
                     PRILVIAHDITDLRRAYRVIEERERSYAYAMEAAGEGIWDWDIDAGTVTHNRRWCELL
                     GYAADTLVHPIAAFADRLHPDDRDEVMGLLDRALAGVGSYEHEHRMRRPDGSVLWVLD
                     RGRVVERAPDGRPLRMAGTVTDVSPRKHNEFALALATDALAHANAELERKVAERTADL
                     ARANEELARLARRDALTGLPNRLAVDERLHEESRRQPGPCGTGCAVLMIDIDSFKPIN
                     DTHGHATGDAVLALVAGLLAATVREADFVSRYGGEEFLVLLPDTALEGARTVAEKLRT
                     VVAETAHPVAGRITISIGLAMAAPGTGELATVVAQADAALYEAKRSGRNRLVIAPPPT
                     AV"
     gene            complement(4985..5488)
                     /locus_tag="PKN25_11395"
     CDS             complement(4985..5488)
                     /locus_tag="PKN25_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNV91547.1"
                     /translation="MAETQQKVKKPAAQATPLSVLQRFRILIRTAQRHSQWIERQSGV
                     TGAQLWALQELREAPGLRVGELANRMALHQSTTSNMVDRLETSGLIRKERTSADQRVV
                     RLHLTDDGFALLSRAPSPARGVLPEALRLMDDEGLASLQSELEKLLRQIKNLDEGFGM
                     QPLPFTE"
     gene            5656..7227
                     /locus_tag="PKN25_11400"
     CDS             5656..7227
                     /locus_tag="PKN25_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNV91548.1"
                     /translation="MAAQPPMSPERNPAAPAQADGARPAAPPPEPPGSAVAPRTAARG
                     RPFARLLRGGRRYALPWLSLRRWRVRLLLVGAALLAGLVAIAFAIGAELAIAAHAGLI
                     RERPWLTLLIAPAGFAALAWISRRFFPGTEGSGIPQAIAASMSDDGRVRRQLLSLRIA
                     LAKVFLTLGGLLSGASVGREGPSVQIGASVLHLLASRKRHRRIASSRDLIVAGSGAGI
                     AAAFNTPLGGIMFAIEEMCRYRAFQANSTTLTAVIFAGLMSLAVLGNYTYFGRTLASL
                     GWPDGIWPVLACGVAGGLLGGIFTRVLVASARGLPGRLGRIAEGQPVAFAAMCGLLTA
                     LIGLASGGVTYGTGYAESKAALEGSAALPAAFTLMKLAVIWLVFASKIPGGIFAPALA
                     VGAGLGADLALLLPGEQDAAILVLGMVAFLAAMTQTPITSFVIVMEMTANHQMLLPLM
                     ATAVVAHGVARSVAPVPLYHALSHPWLRRAEKRAHEKRPRPAHPPVGAQGAGAAKPAV
                     EPPSSADAGAPDQRL"
     gene            complement(7192..8145)
                     /locus_tag="PKN25_11405"
     CDS             complement(7192..8145)
                     /locus_tag="PKN25_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV91549.1"
                     /translation="MNNPNSMFARAMPILFGALFLIGLVAPFAVYPTFLMKVLCFGLF
                     ACAFNLLLGFAGLLSFGHAAFLGSAGYVCGMLVRDLGVTPELGILGGTLAAGALGWVF
                     GVLAIRRTGIYFAMITLALSQMVYFVVLQWKATGGEDGLQGVPRGHLFGVIDLSNNLA
                     MYYLVFAVFCIGFFIIYRAIHSPFGQILKAIRENEPRAMSLGYDVAKFKLLAFVLSAA
                     LAGMAGATKTLVFQLASLTDVHWHMSGEVVLMTLLGGLGTVLGPLVGAATIVSLQTEL
                     ADSVGSWVTVIMGTIFVVCVLLFRRGIVGELQALIRRAGIR"
     gene            complement(8169..9050)
                     /locus_tag="PKN25_11410"
     CDS             complement(8169..9050)
                     /locus_tag="PKN25_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV91550.1"
                     /translation="MEIFGVPSALLGSQLLVGLINGSFYAILSLGLAIIFGLLNIINF
                     THGAQYMMGAFVAWIALNKLGINYWVALLFAPIVIGALGVLIERTMLRKLYKLDHLYG
                     LLLTFGLALIFEGIFRDQYGISGQSYPVPELLQGGVNLGFMFLPIYRGWVVVAALTVC
                     FATWFVIEKTKLGAYLRAGTENAQMVQALGINVPLLITFTYGFGVALAAFAGVLAAPV
                     YQVSPLMGSNLIIVVFAVVVIGGMGSIMGSILTGLGLGLIEGLTRVFYPEASAVVVFV
                     IMVIVLLVRPAGLFGKA"
     gene            complement(9122..10336)
                     /locus_tag="PKN25_11415"
     CDS             complement(9122..10336)
                     /locus_tag="PKN25_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV91551.1"
                     /translation="MQMKKLTTLACAAAFAMLSGAASAQISGNTVKIGVLTDMSGTYS
                     DLAGAGAVLATQMAIEDFIAEAKPAFKVEMVSADHQNKADIASNKAREWFEREGVDTA
                     TELVTTSTALAVMKIAKEMNRVALMSGPASTPITNEQCNDVTVHYTYDTYALANGTAK
                     AVTQQGGNTWFFLTADYAFGQALEKDSSAVVTANGGKVLGSVRHPFPASDFSSFLLQA
                     QASGAKIIGLANAGADTTNAIKQAAEFGVTPKQSLAGLLMFISDVHSLGLQATQGMYL
                     TTGFYWDLDDQTRAWSKRFFEKQKRMPTMVQAGQYSSVLHYLRAVQATGTDEAGKVMA
                     QMKATPIKDFFGKNGRIREDGRMVHDMYLAQVKTPAESKYPWDYYHIRQTIPAEEAFQ
                     PLAQSKCPLVKK"
     gene            complement(10394..11116)
                     /locus_tag="PKN25_11420"
     CDS             complement(10394..11116)
                     /locus_tag="PKN25_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV91552.1"
                     /translation="MTSAFTPHPEMLRIHDLHAFYGESHILHGIDIKVARGECVTLLG
                     RNGSGRSTTLKSVLGLVGKRTGSIMVNGREVIAEPTHRMARYGIGYVPEERAIFASLS
                     TEENLMLPPQVASGALSVEEIYQMFPNLQERRNSPGSKLSGGEQQMLAMARVLRTGAK
                     LLLLDEITEGLAPVIVKKLGEVIVELKSRGFTIVLVEQNFRFAAPLADRHYVLEHGEI
                     IATITAEELPGKMDWLHQTLGV"
     gene            complement(11136..11231)
                     /locus_tag="PKN25_11425"
     CDS             complement(11136..11231)
                     /locus_tag="PKN25_11425"
                     /inference="COORDINATES: protein motif:HMM:NF023816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91553.1"
                     /translation="MLAEGSYAEVSKNPKVLEAYVGSDDIGDAHH"
BASE COUNT         1773 a         4044 c         3771 g         1694 t
ORIGIN      
        1 cgctgctgca gtggcacatc aagtcgacca ccaaggcgca gcagggcctc tacgagtacg
       61 acgcggtctc ccgcctgcgc gactcgcagc tcggcgacga ccgcgtcaag gacatcgcca
      121 actacatcgt caagggcgtg ctgtggcagg ccttcgaggc cgagcagccg accgtggtgc
      181 tgatcgacga gatcgacaag gccgacatcg aattccccaa cgacctgctg cgcgagctcg
      241 accgcatgga gttccatgtc tacgagaccc gccagaccat ccaggcgcgc catcgcccac
      301 tcgtcttcat cacctcgaac aacgagaagg agctgcccga cgccttcctg cggcgctgct
      361 tcttccacta catccgcttc cccgaccgcg acacgatgca gcgcatcgtc gatgtccact
      421 tcccggggct gaagaaggcg ctgctgaccg aggcgatgga ggtcttcttc ggcctgcgcg
      481 acatccccgg cctcaagaag aagccgtcca cctccgagct gatcgactgg ctcaagctgc
      541 tggtggccga ggacatcccg cccgaggcgc tgcgcagcgc cgacggcaag gcggcgatcc
      601 cgccgctgca cggcgccctg ctcaagaacg agcaggacgt gcacctcttc gagcgcctgg
      661 tcttcatggc ccgccacaac cgctgatccg gcgcacgcat ccatgctccg cgccctgctc
      721 ggccggctct tcggccgcaa gcgccccgcc ccctccgtcg cagcgggcga ggcggagggt
      781 ttccgccgcc tccagcgcgc ggcgccgctg cgcgccgagg gggcccccgc gagccccgtc
      841 gaggccggcg ccaccttcct gtgccgcgaa gcggtgctcg ggcgcaacca gcgcatcacc
      901 ggctaccagt tcatgctgca ggcgggcacg cgcgcgcaca tccgccacag cagccgcaac
      961 atccaccacg tcttcgccga agtgctggtg cgcagcctgg tcgacgcgga catcggcgag
     1021 ctgctcggcg aacgcctggc cttcatcgac ctgcccgatt ccttcctgtc ccaccccagc
     1081 ctcgccgccc tcccggcgcg caacatggtg atcgtgccga ccgtgctccc cgatgccggc
     1141 gctcccgagc ccgccgcgct gcgcgccgcc gtgaccgcgc tgcgcgcgcg cggcatgcgc
     1201 atcggcctga tcgatccgac cgtggtcggc gagctcgccc acctgctgcc gcaggccgag
     1261 ttcatcgtcg cccaggccga gggcctcgac gcccgccgcg gcctcaagct cggcaacatc
     1321 gccctcgagg tggcgcacga cgcgcgcctg gtggtgcgcg gcctgccctc gctcgacgac
     1381 ttccgcttct gctgcaagct cggcgccagc ctcttccagg gccccttcat caccagccgc
     1441 gaggactgga gcgcgcgcga ccccggcccc aacaccgcac gcatcggcgc cctgatcgcc
     1501 cgcctgcgcg cagatgccga cacccgcgag ctcgccgacc tgctcaagca ggacgccgcg
     1561 ttgtcgctgc gcctgctgcg ctacatcaac tccgcggcga acgcgctgcc gcagccggtg
     1621 tcctcgatcg aacatgcgct cgcgctgctc ggccgcgagc gcctgcagcg ctggctgatc
     1681 ctgctcgcgt gcaccgcgga caaggcggga gaacgcggcg gcgcggtgct ggagaccgcg
     1741 ctggtacgcg ggcgcatgat ggagctgctg ggtgccggcc gccctgcggc cgagtccgac
     1801 gcgctcttcc tggtcggcct gctgtcgctg atcgacgtga tcctgcaggt gccgctcgac
     1861 aaggcgctcg ccccgctcgc gctgggagcg gaggtcgaag cctcggtacg tggcgaccat
     1921 ggcccctacc aggccctgct cgcgctcacg gtggcgtgcg agcgtggaga ggccggagag
     1981 ggtgcggcgg gcgacgcgct ccgcgccgcc gccgcggcct gcggcatcgc ccccgagcag
     2041 gcctccgaat gccacctgca ggccctgtcc tgggcgatgc agatccagga ctgaccgcgc
     2101 acgaagggaa gaccccatgc tcatcgattt cttcctgcac ctcaaggcac gcaagctgcc
     2161 ggtgtccaca cgcgagttcc tcaccgtgct cgagggcttg cgcgaccacg tctgcgggcc
     2221 ctcgctcgac gacttctact actacgcgcg gctgtgcctg gtgaaggacg agtcgctcta
     2281 cgaccgcttc gaccaggcct tcggcgaata cttcaagggc gtcaccgccc tgcccggcct
     2341 ggaggccgac ctgcccgagg actggctgcg cgcgatggtg aagaagcacc tctcgcccga
     2401 ggagcgcgcc aagctcgaga agctcggctg ggacaagctg atggaggagt tccgcaagcg
     2461 cctgcaggaa cagaagggac gccaccaggg cggcagcaag tggatcggca ccggcggcag
     2521 ctcgcccttc ggcaacaacg gcacccaccc cgagggcatc cgcgtgggtg gcgagtccgc
     2581 cggcaaccgc acggcggtca aggtgtggga caagcgcgag ttccgcaacc tcgacgattc
     2641 ggtcgagctc ggcacgcgca acatcaaggt cgcgctgcgt cgcctgcgcc gcttcgcccg
     2701 ccagggcgcg gccgaggagc tcgacctcga cggcaccatc gccgccaccg cgcgcaacgc
     2761 cggctggctc gacctgatga tgcgccccga acgccacaac gcggtcaagg tgctgctctt
     2821 cctcgacgtc ggcggctcga tggacgacca cgtcaaggtc tgcgaggagc tcttctccgc
     2881 ctgccggctc gagttcaagc acctcgagta cttctacttc cacaactgcg tgtacgaggg
     2941 cgtttggcgc gacaaccgcc gccgccacgc cgagcgtacg ccgctcctcg acgtgatcca
     3001 caagtacggc tcggactaca agctcatctt cgtcggcgat gccaccatga gcccctacga
     3061 gatcctgcat ccgcacggct cggtcgagca catgaacgcc gaggccggcg ccacctggct
     3121 gcgccgcctc ctcgacgcct acccggccgc ggcctggctc aaccccgagc cggagcgcct
     3181 gtggccctac cgccagtcga tcggcatcct cggccagatc atgggcgagc gcatgtaccc
     3241 gatgaccttg gacggcctgg cgcgcgcgat gcaggccctg tcccgccgcc actgatcgag
     3301 gcatcgcacc cccgctccag ccgtcatgga agagaccgaa ctcccctgcc cgttctgcaa
     3361 cgcccacagc acccggccaa tcgcgctccc cgacgggctc tggagcgcgt gctgcgccga
     3421 ctgcggcgca cgcggcccga tcgcgcccga cgcccgcacg gcgctggcgc gctgggaaca
     3481 ggcgacgcgc gacggcgagc tgctgcgcac ggtgatcgac gagtcgcccg acatcatcct
     3541 gctcaaggac tgggatggcc gcttcctgct cggcaacgcc gcgctcgcac gcctgtacga
     3601 caccacgccg gagcaactcg tcggcaagga cgacggcgcc ttcaacccga accgcgagca
     3661 ggtggatttc tacctggaga acgtgcgcgc cgtgatgcgc ggtggtcgca cgcagatcgt
     3721 cgaggagagc tccaccaatg ccgagaccgg cgaggtgcat tacttccact cgatcaagaa
     3781 gccgctgcgc ggtcccgcgg gcgagccgcg catcctcgtg atcgcgcacg acatcaccga
     3841 ccttcgacgt gcataccgcg tcatcgagga gcgcgagcgc agctacgcct atgccatgga
     3901 ggctgccggc gaaggcatct gggactggga catcgatgcc ggcacggtca cccacaaccg
     3961 gcgctggtgc gagctgctcg gttacgccgc cgacaccctg gtgcacccga tcgccgcatt
     4021 cgcggacagg ctgcacccgg acgatcgcga cgaggtgatg ggcctgctcg accgggccct
     4081 cgccggcgtg ggcagctacg agcacgagca ccgcatgcgt cgccccgacg gcagcgtgct
     4141 gtgggtcctc gaccgcgggc gtgtcgtcga gcgcgcaccc gacggccgcc cgctgcgcat
     4201 ggcgggcacg gtcaccgacg tcagcccgag aaaacacaac gagttcgcgc tcgcgctcgc
     4261 gaccgacgcg ctcgcgcacg ccaacgccga gctcgagcgc aaggtggccg aacggaccgc
     4321 cgatctcgcc cgcgccaacg aagagctcgc ccgcctcgcc cgccgcgacg cgctcaccgg
     4381 actgcccaac cgactcgccg tcgacgaacg cctgcacgag gagtcgcgcc ggcagccggg
     4441 accgtgcggc accggctgcg ctgtgctgat gatcgacatc gactcgttca agccgatcaa
     4501 cgacacgcac ggccacgcca ccggcgacgc ggtgctggcc ctcgtcgccg gcctgctcgc
     4561 cgccacggtg cgcgaggctg acttcgtgtc gcgctacggc ggcgaggagt tcctggtact
     4621 gctgcccgat accgccctcg agggcgcgcg caccgtcgcc gagaagctgc gcaccgtcgt
     4681 cgccgagacg gcgcatcccg tggccgggag gatcaccatc tcgatcggcc tggcgatggc
     4741 cgcacccggc accggcgagc tggcgaccgt ggtcgcgcag gccgacgccg cgctctacga
     4801 ggccaagcgc agcggtcgca accggctggt gatcgcgccc ccgccgaccg ccgtctgagc
     4861 cccgtacggc cgcgcggcga caaaaaaatg gcggcctcgc gggccgccga atctgctgtc
     4921 cgcacggagg cggtcacttc atctgaagta cggtcgaggc ggaccgcccc gggcggaccg
     4981 ccgttcattc ggtgaaggga agcggctgca tgccgaagcc ttcgtcgaga ttcttgatct
     5041 ggcgcagcag cttctcaagc tcgctctgca ggctggcgag gccctcgtcg tccatcagcc
     5101 gcaaggcctc gggcagcaca ccccgcgccg gcgaaggcgc cctcgacagc agcgcgaacc
     5161 cgtcgtcggt gaggtggagg cgcaccacgc gctggtcggc gctcgtgcgc tccttgcgga
     5221 tcagccccga ggtctcgagc cggtccacca tgttcgaggt ggtggactgg tgaagcgcca
     5281 tccggttcgc gagctcgccc acgcgcaggc cgggcgcctc gcgcagctcc tgcagcgccc
     5341 acagctgggc gccggtgacg ccgctctggc gctcgatcca ctgggagtgg cgctgcgcgg
     5401 tgcggatcag gatccggaaa cgctgcagga ccgacagggg ggtcgcctga gccgccggct
     5461 tcttcacttt ctgttgcgtt tccgccattc gagtctcgct ggaactatat ttccacaaac
     5521 aatgattgtc aaaaaaacat cactccgctc acgcttgcgt atggtagcgg tatcgttcgt
     5581 tcttcgcatg tgcagcattg cagaaaggcc tctggaaatg ctgctgtcgt ccactccgcc
     5641 gggaaggaat cccgcatggc agcacagcca ccgatgagcc ccgagcggaa ccccgctgct
     5701 cctgcacaag ccgacggcgc gcgccccgct gcgccgcctc ccgagccgcc cggatcagcg
     5761 gtcgcgcccc gcaccgccgc acgcgggcgc ccattcgccc gcctgctgcg cggcgggcgc
     5821 cgctatgccc tgccctggct gtcgctgcgg cgctggcggg tgcgcctgct gctggtgggc
     5881 gcggccttgc tcgccggact ggtcgcgatc gccttcgcga tcggcgccga gctggcgatc
     5941 gccgcccacg ccgggctgat ccgcgagcgc ccctggctca ccctgctgat cgcgcccgcc
     6001 ggcttcgccg cgctggcgtg gatctcgcgc cgcttcttcc ccggtaccga gggcagcggc
     6061 atcccgcagg cgatcgcggc gtcgatgtcg gatgacggcc gcgtgcgccg ccagctgctg
     6121 tcgctgcgca tcgcgctggc caaggtcttc ctgaccctgg gcgggctgct cagcggcgcc
     6181 tcggtgggtc gcgagggccc ttcggtgcag atcggcgcct cggtgctgca cctgctcgcc
     6241 agccgcaagc gccaccgccg catcgcctcc agccgcgacc tcatcgtcgc cggcagcggc
     6301 gccggcatcg ccgcggcctt caacaccccg ctcggcggca tcatgttcgc gatcgaggag
     6361 atgtgccgct accgtgcctt ccaggccaac agcaccacgc tcaccgcggt gatcttcgcc
     6421 ggcctgatgt cgctcgccgt gctgggcaac tacacctact tcggccgcac cctggcgagc
     6481 ctgggctggc ccgacgggat ctggccggtg ctcgcctgcg gggtggccgg cggcctgctc
     6541 ggcggcatct tcacgcgcgt gctggtggcg tccgcacgcg gcctgcccgg ccgcctcggc
     6601 aggatcgccg agggccagcc ggtcgccttc gcggcgatgt gcggcctgct caccgcgttg
     6661 atcggactgg ccagcggcgg cgtcacctac ggcacgggct atgcggaatc caaggccgcg
     6721 ctcgagggca gcgcggcctt gccggcggcc ttcacgctga tgaagctggc ggtgatctgg
     6781 ctggtcttcg cgtccaagat ccccggcggc atcttcgccc ccgcgctcgc ggtgggcgcc
     6841 ggcctgggcg ccgacctcgc cctgctgctg ccgggcgagc aggacgcggc gatcctggtg
     6901 ctgggcatgg tggccttcct cgcggcgatg acgcagacgc cgatcacctc cttcgtgatc
     6961 gtcatggaga tgaccgccaa ccaccagatg ctgctgccgc tgatggccac cgcggtggtg
     7021 gcccacggcg tggcgcgctc ggtggcgccg gtaccgctct accacgcgct ctcccacccc
     7081 tggctgcggc gcgccgagaa gcgggcgcac gaaaaacgcc cgcgccctgc gcaccccccg
     7141 gtgggtgcgc agggcgcggg cgccgcgaag ccggctgtgg aaccgccgtc ctcagcggat
     7201 gccggcgcgc cggatcagcg cctgtagctc gccgacgatg ccgcggcgga acagcagcac
     7261 gcagaccacg aagatcgtgc ccatgatcac cgtgacccag gagcccacgc tgtcggcgag
     7321 ctcggtctgc agcgacacga tggtggcggc gccgaccagc gggccgagca cggtgccgag
     7381 gccgccgagc agcgtcatca gcaccacctc gcccgacatg tgccaatgca cgtcggtgag
     7441 cgaggcgagc tggaacacca gggtcttggt cgcccccgcc atgccggcga gcgcggccga
     7501 gagcacgaag gcgagcagct tgaacttggc cacgtcgtag ccgagcgaca tcgcgcgcgg
     7561 ctcgttctcg cggatcgcct tgaggatctg cccgaagggc gaatggatcg cacggtagat
     7621 gatgaagaag ccgatgcaga acaccgcgaa cacgaggtag tacatcgcga ggttgttcga
     7681 caggtcgatg acaccgaaca gatgtccgcg cggtacgccc tgcaagccgt cctcgccacc
     7741 ggtcgccttc cactgcagca cgacgaaata caccatctgc gacagcgcca gcgtgatcat
     7801 cgcgaagtag atcccggtac ggcggatcgc cagcacgccg aagacccagc cgagcgcgcc
     7861 ggcggcgagc gtgccgccga ggatgccgag ctcgggcgtc acccccaggt cgcgcaccag
     7921 catgccgcac acgtagccgg cgctgccgag gaaggcggca tggccgaagg agagcagccc
     7981 ggcgaagccg agcagcaggt tgaacgcgca cgcgaacagc ccgaagcaca ggaccttcat
     8041 cagaaaggtg ggatacaccg cgaacggcgc caccagcccg atcaggaaca gcgcgccgaa
     8101 caggatcggc atggcgcggg cgaacatcga attggggttg ttcatcagtc tgattccttt
     8161 gcccgcgctc aggccttgcc gaacaggccg gcgggccgca ccagcagcac gattaccatg
     8221 atcacgaaca ccaccactgc cgaagcctcc gggtagaaca cccgcgtcag cccctcgatc
     8281 agccccaggc cgaggccggt gaggatggag cccatgatcg aacccatgcc gccgatcacg
     8341 accaccgcga acaccacgat gatgaggttg gagcccatca gcggactgac ctggtacacc
     8401 ggcgccgcga gcaccccggc gaaggcggcg agcgcgacgc cgaagccgta agtgaaggtg
     8461 atcagcagcg gcacgttgat gccgagcgcc tgcaccatct gcgcgttctc ggtgccggcg
     8521 cgcaggtagg cgccgagctt ggtcttctcg atgacgaacc aggtggcgaa gcacaccgtc
     8581 agcgcggcca ccacgaccca gccgcggtag atcggcagga acatgaagcc gaggttcacc
     8641 ccgccctgca gcagctcggg caccgggtag gactggccgg agatgccgta ctggtcgcgg
     8701 aagatgccct cgaagatcag cgccaggccg aaggtgagca agaggccgta gaggtggtcg
     8761 agcttgtaga gcttgcgcag catcgtgcgc tcgatcagca cgccgagcgc gccgatgacg
     8821 atgggcgcga acagcagcgc gacccagtag ttgatgccga gcttgttgag cgcgatccac
     8881 gccacgaagg cgcccatcat gtactgggcg ccgtgggtga agttgatgat gttgagcagg
     8941 ccgaagatga tggccaggcc caggctcagg atcgcgtaga acgaaccgtt gatgaggccg
     9001 accagcagct ggctgccgag cagcgcgctc ggaacgccga agatttccat gtgcgactcc
     9061 cgcagcggga cggccgtgcc gccccgctgc gtcgtgaagt gcgggacgcc ccgcgatcag
     9121 ctcacttctt gaccagcggg cacttcgact gcgccagcgg ctggaaggcc tcttccgccg
     9181 ggatggtctg gcggatgtgg tagtagtccc acgggtactt cgactcggcg ggcgtcttca
     9241 cctgcgccag gtacatgtcg tgcaccatgc gcccatcctc gcggatgcgg ccgttcttgc
     9301 cgaagaagtc cttgatcggg gtggccttca tctgcgccat gaccttgccc gcctcgtccg
     9361 tgccggtggc ctgcaccgcg cgcaggtagt gcagcaccga ggagtactgg ccggcctgca
     9421 ccatggtcgg catgcgcttc tgcttctcga agaagcgctt ggaccaggcg cgggtctggt
     9481 cgtcgagatc ccagtagaag ccggtggtca ggtacatgcc ctgggtggcc tgcaggccga
     9541 gcgagtgcac gtcggagatg aacatcagca ggccggcgag cgactgcttg ggcgtgacgc
     9601 cgaactcggc cgcctgcttg atcgcgttgg tggtgtcggc gccggcgttg gccagaccga
     9661 tgatcttggc gcccgaggcc tgggcctgga gcaggaagga cgagaagtcg gacgccggga
     9721 agggatggcg taccgagccg agcaccttgc cgccgttggc cgtcaccacc gcggacgaat
     9781 ccttctccag cgcctgaccg aaggcatagt cggcggtcag gaagaaccag gtgttgccgc
     9841 cctgctgcgt caccgccttg gcggtgccgt tggcgagcgc ataggtgtcg taggtgtagt
     9901 gcaccgtcac gtcgttgcac tgctcgttgg tgatcggcgt cgaggccggg ccgctcatca
     9961 gcgcgacgcg gttcatctcc ttggcgatct tcatcaccgc gagcgcggtc gaggtggtga
    10021 cgagctcggt agccgtgtcg acgccctcgc gctcgaacca ttcgcgcgcc ttgttggaag
    10081 cgatgtcggc cttgttctgg tggtcggcgg agaccatctc gaccttgaag gccggcttgg
    10141 cctcggcgat gaagtcctcg atcgccatct gggtggcgag caccgcgcct gcgccggcaa
    10201 ggtcggaata ggtgcccgac atgtcggtga gcacgccgat cttgaccgtg ttgcccgaaa
    10261 tctgcgcact cgccgcgccc gacagcatgg cgaaggcggc cgcgcaggcc agggtggtga
    10321 gtttcttcat ctgcatcgtg ggtctccgtc tcgttcgtgt ggttgttacg cagggactgc
    10381 gcggtgcagc cgctcagacc cccagcgtct ggtgcagcca gtccatcttc ccggggagct
    10441 cctccgcggt gatggtggcg atgatctcgc cgtgctcgag cacgtagtgg cggtcggcca
    10501 gcggggcggc gaagcggaag ttctgctcca ccagcacgat ggtgaagccg cgcgatttca
    10561 gctcgacgat gacctcgccg agcttcttca cgatcaccgg cgccagcccc tcggtgatct
    10621 cgtcgagcag cagcagcttg gccccggtgc gcagcacgcg cgccatcgcc agcatctgct
    10681 gctcgccgcc cgagagcttg gagcctgggc tgttgcggcg ttcctgcagg ttgggaaaca
    10741 tctggtagat ctcttccacc gacagcgccc cgctcgccac ctgcggcggc agcatcaggt
    10801 tctcctcggt gctgagcgag gcgaagatcg cgcgctcctc gggcacgtag ccgatgccgt
    10861 agcgcgccat gcggtgggtg ggctcggcga tcacctcgcg gccgttgacc atgatcgacc
    10921 cggtgcgctt gccgaccagg ccgagcaccg atttcaaggt cgtcgagcgt ccggagccgt
    10981 tgcgcccgag caaggtcacg cattcgccgc gcgccacctt gatgtcgatg ccgtgcagga
    11041 tgtgcgactc gccgtagaag gcgtggaggt cgtggatccg gagcatctcc ggatgagggg
    11101 tgaaggcact ggtcatggcg tttcgggggc ggggatcagt ggtgggcgtc gccgatgtcg
    11161 tcggagccga cgtaggcctc gagcaccttg gggttcttcg acacctcggc gtagctgcct
    11221 tcggcgagca cgctgccgcg ggtgagcacg gtgatgcggt cgcacaggtt ggcgaccacc
    11281 ga
//
LOCUS       DAOQBS010000104        11133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_5070_length_11133_cov_14.207528, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000104 DAOQBS010000000
VERSION     DAOQBS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 11133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11133
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5070_length_11133_cov_14.207528"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..385
                     /locus_tag="PKN25_11430"
     CDS             107..385
                     /locus_tag="PKN25_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HNV91554.1"
                     /translation="MTAAQPKNPLHGLTLERIVTDLVACFGWAGLGRRIDIRCFNHEP
                     SIASSLKFLRRTPWAREQVEALYLDTLRAAARPGAATDPDTPRSTPNT"
     gene            382..1239
                     /locus_tag="PKN25_11435"
     CDS             382..1239
                     /locus_tag="PKN25_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HNV91555.1"
                     /translation="MKTPSRIEPLVADGLVDHVLRQLMSGKEAMVYVVQCGDELRCAK
                     VYKEANKRGFHKAADYTEGRKVKNSRQARAMAKGSRYGRQEQEAAWQHAEVDALRRLA
                     AAGVRVPVPYDFHEGVLLMELVADEDGEPAPRLNDVVLDEADARRFHAELVRQVVRML
                     CAGIVHGDLSEYNVLVDRDGPVIIDLPQAVDAAANNNARAMLERDVGNLAAYFGQFAP
                     DLLDTAYGKEIWALYESGKLQPESALTGVFEVEAGHADVDEVMQVIEAARREEAARQA
                     GRAEAAGQR"
     gene            complement(1475..2380)
                     /locus_tag="PKN25_11440"
     CDS             complement(1475..2380)
                     /locus_tag="PKN25_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNV91556.1"
                     /translation="MNPLVRIQPRSADLGDGMRVRRTLPNRQQRMVGAWCFLDHAGPV
                     HFEPGKGLHVGAHPHIGLQTFTWLIEGEVLHRDSLGNEQLIRPGQVNLMTAGHGIAHT
                     EDSLHDGVRLHAAQLWIALPPEHQDCAPAFDHYPTLPQWREGGAELTLLAGRYGQRTA
                     PARIHSALVGIDIACDEASALTLALDPSFEYGLMALVGDLKIDGETIRTDELAYLGKG
                     RGELHLALPAGARVLFLGGEPFDEPVQMWWNFVGFSRADIAQAQAAWERGDERFGAVG
                     DGRARRLVAPPLPWRGPGDTRLPHT"
     gene            2595..2930
                     /locus_tag="PKN25_11445"
     CDS             2595..2930
                     /locus_tag="PKN25_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91557.1"
                     /translation="MNWIMAVSIGFCVLLSSGTAMAAGAGQEGGMASVQAPAQQQEKK
                     EGAKTAVTEADPGNRASLLYIVLIGGVMVVAMKMAIAYGKKKLDEEREAAREKAAREE
                     RQNKSQAQG"
     gene            3175..4089
                     /locus_tag="PKN25_11450"
     CDS             3175..4089
                     /locus_tag="PKN25_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNV91558.1"
                     /translation="MVRALYSRAGSHAGVGLLLLVLFFLYAERAFGLLPADHAVQAWL
                     DPVRNAALALALFTGAHLLHALASARLSQRRKGRKPVPKVLLDLLRVFLFALAALGGL
                     SLLFRQDLSGILTGSGLVLAVLGFAIRNVVADTLSGIALGIEAPFRIGDWVRIENLAQ
                     GRVQEIGWRTTRLVTGDSTYVILPNSQISRQRITNFSAPKKEYRDHAELTLPVALPVA
                     EARELMRAALTHAASIAEDKPPEVQLVSYGPGGITYRVKYWVPQHDREPVCRDEVLGL
                     VDAALRARGVVLAQPLAVSACQHAEAAS"
     gene            4161..5081
                     /locus_tag="PKN25_11455"
     CDS             4161..5081
                     /locus_tag="PKN25_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV91559.1"
                     /translation="MSTEVVDKSAAYGLVRFLLESFRGVLDTGEERIMRQACASILAT
                     AALVTALGSGTAEAAVLDVTVRGSDAIFLAGRTDITIPLASAPWSSPTGMQRHGGATP
                     EEIQETFPPYLGVAAGDVIRLADPSIGGISYFNGVGAPFYGPEGNNADCAGSSCSDLS
                     SFGGISGYFGPEGALVGVFLDNSIPSAGAPARLDFSTTGLGRNFTTLSPGLGQIFYIG
                     NGVTTSSIFQEFIAPSGATRLFLGIPDGFGFDGVPGAYDDNDGAYRVRIGVNEVPTVP
                     EPGVLALLGLGLVGLAAARLRTPQGSKVPG"
     gene            complement(5160..7037)
                     /locus_tag="PKN25_11460"
     CDS             complement(5160..7037)
                     /locus_tag="PKN25_11460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV91560.1"
                     /translation="MRQTQEFPPLNMSTPSNPSEIARETLRQLALRRIAPTPDNYRKL
                     YHEIAGTRPDDDALPEAFVRKLARRLPRDSAERQRHGHQLDQALADGRDKAAEAALER
                     YLDSLKLGEPQAWNELIGNLLRQWEARQTGWTPARKREALDRVLGAADPAALYTRLQG
                     LLKSWTQAMTDADALAVEVPDAADEPDASASPRADASTARPATPARTAAAAPPPASIP
                     GVDSATVDALRELVTISLGEALPPLLEEQPPLRETAATLCAEAASRSEAAGLREVGTR
                     LRAFAHELELAAADEAEIRAGMLELLRMLLRNIDELVLDDRWLAGQIEMLREIVDSRP
                     DPRKIDDAGRRLKELIHKQGQLKHTLAQSQRHLRDMLAGFVDQLARFSESTGAYHDRI
                     GQCAQRIAQANDITEIGPLLDEVMTETRSIQDEARRSRDELIVARGEATAAQERVVTL
                     QNELEEASRLMRHDQLTGALNRRGLDEMFQTESARAQRRGTRLAVALLDIDHFKKLND
                     SLGHKGGDDALVHLAKVVRQHLRPQDVLARYGGEEFVILLPETGDTDAQQALVRLQRE
                     LTREFFMAEMQKIVITFSAGVTRLAEGESLESALKRADGAMYQAKQAGRNRVMVAPLH
                     D"
     gene            7209..8837
                     /locus_tag="PKN25_11465"
     CDS             7209..8837
                     /locus_tag="PKN25_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HNV91561.1"
                     /translation="MGRRTFDWRRWIGGALILATSIMAGAASTPAGGDLPVAAAGGKA
                     ASTADHSRFKELQGPFESGPEVTKACLACHNEAGHQVMKSVHWTWEAISPTTGKKLGK
                     QHAANNFCGSILSNEPRCTSCHAGYGWTDKNYDFTNQNNVDCLACHDTTTTYKKIATD
                     AGHPLYAPRELPKGSGRIVQPPDLARIAQAVGKPGRHNCGACHFNGGGGDAVKHGDLD
                     SSLVKPPKHVDVHMSPDGENMSCADCHTFNAHQPSGSRYAATAKDTKGLDMKHEDHKR
                     ATCESCHDLRPHKNERLDNHARRVACQTCHIPEFARGGIATKVLWDWSTGGKRGPDGK
                     PLFIQDDHGHLIYSAEKGDFKYGENVRPTYKWYNGVVHQIALTDKIDDSKILELNRVE
                     GSASDPNARIWPFKVMVGKQPYDPVNKTLVVNHVYGQDDTAFWGNFDYAKSIKAGMDY
                     AGLPYSGQFGFIETRMNWFITHMVAPKEDALKCRDCHTRSEDGRLAGITDLYLPGRDR
                     NGWVDLLGGLAIIGAIGAGSIHGIARILTRRKGH"
     gene            8840..9496
                     /locus_tag="PKN25_11470"
     CDS             8840..9496
                     /locus_tag="PKN25_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNV91562.1"
                     /translation="MSANRIYIYKRYERFWHWSQALLIIVMMITGFEIHGSYTLLGFR
                     KAVDAHTIAAWTLLVIWAFTIFWQFTTGEWRQYVPSMKNVSAMVLYYTVGIFKEMPHP
                     FRKTTVQKHNPLQRLAYLALLAFISPLIWGSGLLYLFYGNWAQLGIDEMFSLEGVALV
                     HTAGAFMITAFFFIHVYLTTTGHTIFAHIKAMITGWEEVDDDHPPAAAEQGGRTPGAA
                     "
     gene            complement(9551..9883)
                     /locus_tag="PKN25_11475"
     CDS             complement(9551..9883)
                     /locus_tag="PKN25_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91563.1"
                     /translation="MEKRWRRLPGSRTEPAGFERKVLARLPMVALVGTAACVAVSLLA
                     RLHWWGDESIEAVRALQMVDIWVLAAVILHWTVVFTVAIGAFIVFVMKGPAYIADRYE
                     LPDADAPR"
     gene            10118..10576
                     /locus_tag="PKN25_11480"
     CDS             10118..10576
                     /locus_tag="PKN25_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HNV91564.1"
                     /translation="MTLQQAETAAPAPLSTNAQDQAKQPRIEPRAILKRLQAESPAFR
                     DSKPLALKIDTSILARFPDFDRKNLRIALRMYTASTKYLKAVERGGERFDLDGNASGE
                     VTEEQRQHAATTLKERFAAVAKQQREKREAEEAERRRTEKLQQLVSKFGR"
     gene            complement(10589..10753)
                     /locus_tag="PKN25_11485"
     CDS             complement(10589..10753)
                     /locus_tag="PKN25_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91565.1"
                     /translation="MQTITSRVLLALGLLAGLMIGTGHPGGALVVAVAAGAGIWMARA
                     DSVRRDLRRA"
     gene            10899..10974
                     /locus_tag="PKN25_11490"
     tRNA            10899..10974
                     /locus_tag="PKN25_11490"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10932..10934,aa:Thr,seq:tgt)
     gene            11052..11128
                     /locus_tag="PKN25_11495"
     tRNA            11052..11128
                     /locus_tag="PKN25_11495"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11086..11088,aa:Met,seq:cat)
BASE COUNT         1778 a         3605 c         3933 g         1817 t
ORIGIN      
        1 cgcccgttac accccggatt gacaacgcgc gccagcgcgg agtatcagtc actccccctg
       61 cagcaagcga tccgcacaac cgtcagtctc gacgcgagaa gacccgatga ccgcagcaca
      121 acccaagaat ccgctccatg gcctgacgct cgaacggatc gtgaccgacc tggtcgcctg
      181 cttcggctgg gcgggcctgg ggcggcggat cgacatccgc tgcttcaacc acgaaccgag
      241 catcgcctcg agcctcaagt tcctgcgtcg cacgccgtgg gcgcgcgagc aggtggaggc
      301 gctgtatctc gataccctgc gtgcggccgc ccggccgggc gcggcaacgg accccgacac
      361 cccgcggagc accccgaaca cgtgaagacc ccgagcagaa ttgaaccgct ggtggccgac
      421 ggcctcgtcg accacgtcct gcgccagctc atgagcggca aggaggccat ggtctatgtc
      481 gtgcagtgcg gcgacgagct gcgctgcgcc aaggtgtaca aggaggccaa caagcgcggc
      541 ttccacaagg cggccgacta caccgagggg cgcaaggtca agaacagccg tcaggcgcgc
      601 gcgatggcca agggctcgcg ctacggccgc caggagcagg aggcggcgtg gcagcacgcc
      661 gaggtcgacg cgctgcggcg gctcgccgcg gccggggtgc gcgtgcccgt gccctacgac
      721 ttccacgagg gcgtgctgct gatggagctg gtggccgacg aggacggcga gccggcgccg
      781 cggctcaacg atgtggtgct cgacgaggcg gatgcgcggc gcttccatgc cgagctggtg
      841 cgccaggtcg tgcgcatgct gtgcgccggc atcgtccatg gggatctgtc ggaatacaac
      901 gtgctggtcg atcgcgacgg gccggtgatc atcgacctgc cgcaggcggt ggatgcggcg
      961 gccaacaaca acgcccgcgc catgctcgag cgcgatgtcg gcaacctcgc cgcctacttc
     1021 gggcagttcg cccccgacct gctcgacacc gcgtatggca aggagatctg ggccttgtac
     1081 gagtcgggca agctgcagcc cgagagcgcg ctcaccgggg tcttcgaggt ggaggccggg
     1141 cacgccgatg tcgatgaggt gatgcaggtg atcgaggcgg cgcgtcgtga ggaggccgcg
     1201 cgccaggcgg gccgggcaga ggccgccggc cagcgctgag cggcgaggcg gactccgatg
     1261 cgcgcttgcc cgggcgtctc gcccgacggg gtcgagtgcg atcggccgat ccgatgttcc
     1321 attggtcggt ccggatcgtc ggtgacgtgc gtcgcagact cgacagcatt cgtacgcgcg
     1381 cggaccgcgc gcaggctgcc cggctcgagg gctggctggg ggcggtgctt gccgaccgct
     1441 cggaccggag tccgggtttc gacctcgact gcgctcaggt gtgggggagg cgcgtgtcgc
     1501 ccggcccccg ccatggcaga ggcggcgcca cgaggcgtcg cgcccggccg tcaccgaccg
     1561 cgccgaagcg ctcgtcgccg cgctcccacg cggcctgcgc ctgcgcgatg tcggctctgc
     1621 tgaagccgac gaagttccac cacatctgca ccggttcgtc gaacggctcg ccacccagga
     1681 acagcacccg cgcgcctgcg ggcagggcca ggtgcagctc gccgcgtcct ttgccgaggt
     1741 aggcgagctc gtctgtacgg atcgtctcgc cgtcgatctt cagatcgccc acgagcgcca
     1801 tcagcccgta ctcgaaactg gggtcgagcg cgagcgtgag cgcgctcgcc tcgtcgcagg
     1861 cgatgtcgat gccgaccagc gcggagtgga tgcgcgccgg cgcggtccgt tggccgtagc
     1921 ggccggcgag cagggtgagt tcggcgccgc cttcgcgcca ttgcggcagc gtggggtagt
     1981 ggtcgaaggc cggggcgcaa tcctggtgct ccggcggcag ggcgatccac agttgcgcgg
     2041 cgtgcagccg cacgccgtcg tgcagcgaat cctcggtatg ggcgatgccg tggcctgcgg
     2101 tcatcaggtt gacctgtccc gggcggatga gctgctcgtt gcccaggctg tcgcggtgca
     2161 gcacctcgcc ctcgatcagc caggtgaagg tctgcaggcc gatatgcgga tgcgcgccga
     2221 cgtgcaggcc cttgccgggc tcgaagtgca ccggcccggc gtggtcgagg aagcaccacg
     2281 ccccaaccat gcgctgctgg cgattgggga gggtgcggcg gacgcgcatg ccgtcgccga
     2341 ggtcggcgct gcggggctgg atgcggacga gcgggttcat ggtcggtgtc ctcgtggggc
     2401 tgctgccttg ggcgtcgtgg ggattccccg tggagagatt cttgcggcgc gtcgaccgcg
     2461 gtgggctgtt cgggtgccga gtcccggcgg ggttcttcag tacaatgacc agaggctcga
     2521 cagatcgatg tgtcgaggcg ccgctcgctg gaaaagtgga cagcgcgtga cgagatcgac
     2581 gaatgaggat ttcgatgaat tggatcatgg cagtgtcgat cggtttctgt gtcctccttt
     2641 cgagtggcac ggccatggcc gcgggcgccg gacaggaagg ggggatggcg agcgtgcagg
     2701 cgcccgcgca gcagcaggag aagaaggaag gcgcgaagac ggcagtgacg gaggcggacc
     2761 ccggcaatcg agcttcgttg ctgtatatcg tgctgatcgg tggggtgatg gtcgtggcca
     2821 tgaagatggc gatcgcttac ggcaagaaga agctggacga ggagcgcgag gccgcccgag
     2881 agaaagctgc gcgtgaggag cgacagaaca agtcgcaagc gcaggggtga ggcaggcggg
     2941 gccctgcgtt tgaaaacgcg gaatcgatcc ccatatagaa acgattggcc atcctggcca
     3001 cccaccccca ggaacttccg gagaccatgt gatgggctgc tgcggccgcg cgagattcgc
     3061 cttgtgcatc gaccaggcac atgcctggcc ccggcctcga tagcgccttg ccggcgcgga
     3121 tcggcaggcc ggggtcggtg gagtgtcttt tcctctcgtc tcgaaggtcc tgccatggtt
     3181 cgcgcgctgt actcccgcgc cggctcgcat gccggcgtcg gtctcctcct cctggtgttg
     3241 ttcttcctgt atgccgagcg ggcgttcggg ttgctgccgg ccgatcatgc cgtgcaggcc
     3301 tggctcgatc cggtgcgcaa tgcggcgctc gcgctcgccc tgttcaccgg cgcgcatctg
     3361 ctgcacgcgc tcgcgtcggc gcggctgtcg cagcggcgca aggggcgcaa gccggtgccc
     3421 aaggtgctgc tggacctgct gcgggtgttc ctgttcgcgc tggcggcgct gggcgggctg
     3481 tcgctgctgt tccgccagga cctgtcgggg atcctgaccg gctcgggcct ggtgctggcg
     3541 gtgctgggct tcgcgatccg caacgtggtg gccgacacgc tgtcggggat cgcgctcggg
     3601 atcgaggcgc cgttccgcat cggcgactgg gtgcgcatcg aaaacctggc ccaggggcgg
     3661 gtgcaggaga tcggctggcg cacgacgcgg ctggtgaccg gggacagcac ctacgtgatc
     3721 ctgcccaaca gccagatctc gcggcagcgg atcaccaatt tcagcgcgcc gaagaaggaa
     3781 tatcgcgacc atgccgagct caccctgccg gtcgcgctgc cggtggccga ggcgcgggag
     3841 ctgatgcgcg cggcgctgac gcatgcggcg agcatcgccg aggacaagcc gcccgaggtg
     3901 cagctggtgt cctatggccc tgggggcatc acctatcggg tgaagtactg ggtgccgcag
     3961 cacgaccgcg agccggtctg ccgcgacgag gtgctcgggc tcgtcgatgc ggcgctgcgc
     4021 gcgcgcgggg tcgtgctggc gcagccgctg gcggtgtccg cgtgccaaca tgcggaagcc
     4081 gcgtcctagg aacaggcttg cctgcgcggc tcgtcggggc ggggtgtggc gctgtcggtc
     4141 ttttttacac tcggagacgg gtgtcgacgg aagtcgtcga taaatcagcg gcttacggtt
     4201 tggttcggtt cttgcttgag tcttttcggg gagtgttgga taccggggag gagcgaatca
     4261 tgaggcaggc ttgcgcatcg atccttgcaa ctgcggcgct ggtcacggcg ctcggttccg
     4321 gcacggcaga ggcggccgtg ctcgatgtca cggttcgcgg cagcgacgcg atcttcctgg
     4381 cggggagaac ggacatcacg attccgctgg cgtctgcgcc ttggagctct cccaccggca
     4441 tgcaacgcca cgggggggcg acgcccgagg agatccagga gaccttcccg ccatacctcg
     4501 gcgtggctgc gggcgacgtc atccgcctcg cggatccctc gatcggcggc atcagctact
     4561 tcaacggggt cggtgcgccg ttctatggcc ccgagggcaa caacgccgat tgcgcggggt
     4621 cgagctgcag cgatctctcg tccttcggcg gcatcagtgg ctacttcggt cccgaggggg
     4681 cactcgtcgg cgtcttcctg gacaacagca tccccagcgc tggcgcaccc gcgcgcctcg
     4741 acttcagcac gaccggcctg ggccgcaact tcacgacact gagccccggg ctcgggcaga
     4801 tcttctatat cggcaacggc gtgaccacgt cctcgatttt ccaggagttc atcgcgccga
     4861 gcggcgccac gaggctgttc ctggggatcc cggacggctt cggcttcgat ggcgtgcccg
     4921 gtgcctacga cgacaacgat ggcgcctacc gcgtccgcat cggcgtcaac gaggtgccca
     4981 ccgtgccgga gccgggcgtg ctcgcgctcc tcgggctggg cctcgtcggc cttgccgccg
     5041 cgcgcctgcg cactcctcaa gggagcaagg tgccgggctg agcacgccgc cgcgccggct
     5101 tcgccacggg ctcggcgccc ttggcgaagc cggcgggttt cgtgtgctcc ggagcgcttt
     5161 cagtcgtgta gcggcgcgac catcacgcgg ttgcggccgg cctgcttggc ttggtacatc
     5221 gcgccgtcgg cgcgcttcag cgcgctctcc aggctttcgc cctcggccag gcgggtgacg
     5281 ccggcgctga aggtgatcac gatcttctgc atttcggcca tgaagaattc gcgggtgagc
     5341 tcgcgctgca ggcgcacgag cgcctgttgg gcgtcggtgt cgccggtctc gggcagcagg
     5401 atgacgaact cctcgccacc atagcgggcg agcacgtcct gggggcgcag gtgctggcgc
     5461 acgaccttgg cgaggtgtac cagggcgtcg tcgccacctt tgtggccgag gctgtcgttg
     5521 agcttcttga agtggtcgat gtcgagcagc gcgacggcga gccgcgtgcc gcgacgctgg
     5581 gcgcgcgcgg attcggtctg gaacatctcg tcgaggccac ggcggttgag cgcgccggtg
     5641 agctggtcgt ggcgcatcag gcggctggct tcctcgagct cgttctgcag cgtgacgacg
     5701 cgctcctgcg ccgccgtggc ctcgccgcgg gcgacgatga gctcgtcgcg cgagcggcgt
     5761 gcctcgtcct ggatgctgcg agtctcggtc atgacctcgt cgagcagggg gccgatctcg
     5821 gtgatgtcgt tggcctgggc gatacgctgg gcgcactggc cgatgcggtc gtggtaggcg
     5881 ccggtcgatt cggagaagcg cgcgagctgg tcgacgaagc cggcgagcat gtcgcgcagg
     5941 tggcgctggg actgggccag ggtgtgcttg agctggccct gcttgtggat gagttccttg
     6001 aggcggcggc cggcgtcgtc gatcttgcgc gggtcgggac ggctgtcgac gatctcgcgc
     6061 agcatctcga tctggccggc gagccagcga tcgtcgagca ccagttcgtc gatgttgcga
     6121 agcagcatgc gcagcagctc gagcatgccg gcgcggattt cggcctcgtc ggcggcggcc
     6181 agctcgagct cgtgtgcgaa ggcgcgcagg cgcgtgccga cctcgcgcaa gccagcggcc
     6241 tcgctgcgac tggcagcttc ggcgcacagc gtggcggcgg tctcgcgcag cggcggttgt
     6301 tcctccagca gcggcggcag ggcctcgccc aggctgatgg tgacgagctc gcgcagggcg
     6361 tcgacggtgg cggagtcgac gcctggaatg gaggcagggg ggggcgcggc agcggcggtg
     6421 cgcgcgggcg tggcgggcct tgcggtgctt gcgtcggcac ggggcgaagc gctggcgtcg
     6481 ggttcgtctg ccgcgtcggg cacctcgacg gcgagcgcat cggcatcggt catggcctgc
     6541 gtccagctct tgagcaggcc ctgcaggcgg gtgtacagcg ccgccgggtc ggctgcgccc
     6601 agcacgcggt cgagggcctc gcgcttgcgc gccggggtcc agccggtctg acgcgcctcc
     6661 cactggcgca gcaggttgcc gatgagctcg ttccaggcct gcggttcgcc cagcttgagg
     6721 ctgtcgaggt agcgctcgag tgcggcctcg gcggccttgt cgcgaccgtc ggcgagcgct
     6781 tggtcgagct ggtggccgtg gcgctggcgc tcggcgctgt cgcgcggcag gcggcgggcg
     6841 agtttgcgca cgaaggcttc gggcagggcg tcgtcgtcgg gccgggtgcc ggcgatctcg
     6901 tgatagagct tgcggtagtt gtccggcgtg ggggcaatcc ggcgcagcgc gagctggcgc
     6961 agggtttcgc gggcgatctc ggagggattg ctgggcgtgg acatgttgag cggtggaaat
     7021 tcctgggtct ggcgcatgtt tccggcattc tgacaggact tcccgtgctt tcggtgagga
     7081 agttccgagg gcgggcacgg agggagtcga gccccggtga tctgccgatc cgtagatatt
     7141 aatcaaatgc ttatatttct ggctggccta gactggaaaa gcaggtcgtc tcggcggagg
     7201 aacggaaaat gggcaggcgg acattcgact ggcggcgctg gatcggcggg gccttgatcc
     7261 tcgcgacgag catcatggcg ggtgcggcaa gcacgcccgc agggggggat cttccggtgg
     7321 ctgcggccgg cggcaaggcg gcgagcacgg cggaccacag caggttcaag gaactgcagg
     7381 gcccgttcga gagcgggccg gaggtcacca aggcctgtct cgcctgtcac aacgaggccg
     7441 ggcaccaggt gatgaagagc gtgcactgga cctgggaggc gatcagcccg accaccggca
     7501 agaagctggg caaacagcac gcggccaaca acttctgcgg ctccatcctt tccaacgagc
     7561 cgcgctgcac gagctgccac gccggctacg ggtggacgga caagaactac gatttcacca
     7621 accagaacaa cgtggattgc ctggcctgcc acgacacgac gacgacctac aagaagatcg
     7681 ccaccgatgc cggccatccg ctctacgcgc cgcgcgagct gcccaagggc agcggcagga
     7741 tcgtgcagcc gcccgacctg gccaggatcg cgcaggcggt gggcaagccg gggcggcaca
     7801 actgcggcgc gtgccacttc aacggcggtg gcggcgacgc ggtcaagcat ggcgacctcg
     7861 actcctcgct ggtcaagccg ccgaaacacg tcgatgtgca catgtcgccc gatggcgaga
     7921 acatgagctg tgcggactgc cacaccttca acgcccacca gccctccggc agccgctacg
     7981 cggctaccgc caaggacacc aagggcctgg acatgaagca tgaggaccac aagcgcgcga
     8041 cctgcgagtc ctgccacgac cttcgcccgc acaagaacga gcgcctcgac aaccacgcca
     8101 ggcgagtggc ttgccagact tgccacatcc ccgagttcgc gcgcggcggc atcgccacca
     8161 aggtgctgtg ggactggtcg accggcggca agcgcggtcc ggacggcaag ccgctgttca
     8221 tccaggacga ccacggccac ctgatctact cggcggaaaa gggcgacttc aagtacggcg
     8281 agaacgtgcg cccgacctac aagtggtaca acggcgtggt ccatcagatc gcgctcaccg
     8341 acaagatcga cgacagcaag atcctcgagc tgaaccgcgt cgaaggctcg gcgagcgacc
     8401 cgaacgcgcg catctggccc ttcaaggtga tggtcggcaa gcagccctac gacccggtga
     8461 acaagactct ggtggtgaac cacgtatacg gccaggacga caccgcgttc tggggaaact
     8521 tcgactacgc caagtcgatc aaggccggca tggactacgc cggcctgccc tacagcggcc
     8581 agttcggctt catcgagacg cgcatgaact ggttcatcac ccacatggtg gcgcccaagg
     8641 aagacgcgct caagtgcagg gactgccaca cccgctcgga agacggccgc ctcgccggga
     8701 tcaccgacct ctacctgccc ggtcgcgacc gcaacggctg ggtcgatctc ctcggtgggc
     8761 tggcgatcat cggcgcgatc ggcgccggct cgatccacgg catcgcccgc atcctcaccc
     8821 gtcgcaaggg gcactgaaaa tgagcgcgaa ccgcatctac atctacaagc gctatgaacg
     8881 cttctggcac tggtcgcagg cgctgctgat catcgtcatg atgatcaccg gcttcgagat
     8941 ccacggcagc tatactcttt tgggcttcag gaaggccgtg gatgcgcaca cgatcgcggc
     9001 atggacgctg ctggtgatct gggccttcac gatcttctgg caattcacca cgggcgagtg
     9061 gcggcagtac gtcccgtcga tgaagaacgt gtcggcgatg gtcctctact acacggtggg
     9121 gatcttcaag gagatgccgc atcccttccg caagacgacg gtgcagaagc acaacccgct
     9181 gcagcgcctg gcctatctcg cgctgcttgc gttcatctcg ccgctgatct ggggctcggg
     9241 cctgctttac ctgttctatg gcaactgggc gcagctcggc atcgacgaga tgttctcgct
     9301 cgagggcgtg gcgctcgtgc ataccgcagg tgcgttcatg atcaccgcgt tcttcttcat
     9361 ccacgtgtat ctgacgacca cggggcacac gatcttcgcg cacatcaagg cgatgatcac
     9421 cggctgggaa gaggtcgatg acgaccaccc gcccgccgcg gccgagcagg gcgggcgcac
     9481 acccggcgcg gcttgagtgc ttgcgccgta tcgcggcacc accaagggac gcagacgccc
     9541 cgcggcggat tcagcgcggg gcgtctgcgt ccgggagttc gtagcggtcg gcgatgtagg
     9601 ccgggccctt catcacgaac acgatgaagg caccgatcgc cacggtgaac accaccgtcc
     9661 aatgcaggat caccgctgcc agcacccaga tgtcgaccat ctgcagcgcg cgcaccgcct
     9721 cgatgctctc gtcgccccac cagtgcagcc gggcgagcag ggacacggcc acgcaggccg
     9781 cggtgccgac gagcgcgacc atgggcaggc gcgccagcac cttgcgttcg aatcctgccg
     9841 gttcggtgcg cgagccgggc aggcggcgcc agcgcttttc catgatgttt ccccgaggtg
     9901 tgtggtgcgg cagcaagccg gttccgttcg atccgatgcc tgggtgccag accgatgcga
     9961 tccgtggcaa aaatcaccac gtccggcaga agcccggcgg accgggggta gaatgccgga
    10021 acagcactcg actcgcacag ttcgcggcac cgctccggac gcgaggtttc cggactgctg
    10081 ttcagacacc gatccgcgta ccagagttct ccccgaaatg acccttcagc aagccgaaac
    10141 cgccgccccc gcgcccctca gcaccaacgc gcaggaccag gccaagcagc cgcgcatcga
    10201 accgcgtgcc atcctcaaac gcctgcaggc cgaatcgccg gcatttcgcg acagcaagcc
    10261 gttggcgctg aagatcgaca cgagcatcct ggcccgcttt cccgatttcg accgcaagaa
    10321 cctgcgcatc gccctgcgca tgtacaccgc ctcgaccaag tacctcaagg cggtcgagcg
    10381 tggcggcgag cgcttcgacc tcgacggcaa cgcctccgga gaggttaccg aagagcaacg
    10441 ccagcacgcg gccaccacgc tgaaggaacg cttcgccgcg gtggccaagc agcagcgcga
    10501 gaagcgcgag gccgaggagg ccgaacgccg ccgcaccgag aagctccagc aactggtgag
    10561 caagttcggc cgctgaaccc gggcgggcct acgctcggcg cagatcgcgc cgtaccgagt
    10621 cggcccgcgc catccagatc cctgccccgg ccgcgaccgc caccacgagc gccccgccag
    10681 gatggccggt tccgatcatc aggccggcga gcaggccgag tgcgagcagc accctggaag
    10741 tgatggtttg catggtggtc tcctgagctt ttcgactgtg caacaactgt atatataaac
    10801 agttatccgt gcaagttcgt gtgcatgcag gggctgcccg tgcgtttcct ccgacgcgat
    10861 ggctgagtgg cgtcttgcgc tataatccgc accgccttgc cggcatagct cagttggtag
    10921 agcagctgat ttgtaatcag aaggtcgtgg gttcgattcc tattgccggc accagaattc
    10981 ctccggtgct tgtgcggcgc aaaaagttct tgccttctcg tagttcaccg tttatagttc
    11041 tgtttctccg acgcggggtg gagcagtctg gcagctcgtc gggctcataa cccgaaggtc
    11101 gcaggttcaa atcctgcccc cgcaaccaaa aaa
//
LOCUS       DAOQBS010000105        10921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_5246_length_10921_cov_14.947819, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000105 DAOQBS010000000
VERSION     DAOQBS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10921
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5246_length_10921_cov_14.947819"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKN25_11500"
     CDS             complement(<1..392)
                     /locus_tag="PKN25_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNV91566.1"
                     /translation="MSCLASLLLRAAVVSLAVLILHPAQAYAAAERIKDLASVAGVRD
                     NQLVGYGLVVGLDGSGDQTTQTPFTVQSIVNMLGNLGVSLPPGTNLQLKNVAAVMVTA
                     NLPAFARPGQAIDVTVSSIGNAKSLRGGT"
     gene            complement(403..1080)
                     /locus_tag="PKN25_11505"
     CDS             complement(403..1080)
                     /locus_tag="PKN25_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNV91567.1"
                     /translation="MKLARHSIAVGLLALLSGCAAIQTTPPSAVHQPMSVRPEAMATA
                     LPANGAIYQTVQARPLFEDRRARRIGDTITINLVERNTAEKSANANATRNGNMSAGIG
                     PINRLPLSGLNGLELEADAESDFNGKGAAAANNVFNGTITVTVIDVYPNGNLLVSGEK
                     MVAINQGNEFIRFSGVINPNTVTTANTVQSTQVADARIEYRGSGFIDESNTMGWLQRF
                     FVAIMPF"
     gene            complement(1089..1871)
                     /gene="flgG"
                     /locus_tag="PKN25_11510"
     CDS             complement(1089..1871)
                     /gene="flgG"
                     /locus_tag="PKN25_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNV91568.1"
                     /translation="MIRSLWTARTGLDAQQTSLDVISNNLANVSTNGFKRQRAIFEDL
                     LYQTIRQPGAQSTQQTQISSGLQIGTGVKPVATERIFTQGTLQQTGGSLDVAVQGNGF
                     FQVAMPDGTTAYTRDGAFQLDSQGNVVTASGYTLADNINIPADALSITIGKDGTVSVL
                     QAGQTAPVEIGAIQLATFINSGGLQAAGENLFYETASSGVATPTQPGTNGAGVLNQGY
                     VEVSNVNVAEELVSMIVTQRAYELNSRAISTSDQMLGRLTQL"
     gene            complement(2120..2860)
                     /gene="flgF"
                     /locus_tag="PKN25_11515"
     CDS             complement(2120..2860)
                     /gene="flgF"
                     /locus_tag="PKN25_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HNV91569.1"
                     /translation="MDRLIYTAMTGAKGTMDQQAAVAHNLANVDATGFRAELHKLRAV
                     EVQTEALRTRAFTVDASVANDFTEGPLQFTGRPFDVALAGKGWLAVQMPDGSEAYTRN
                     GSLAVSANGVLQTRDGRPVLGDGGPVTIPPDNEITIGADGSVSASQPDQPGVVNVVAQ
                     LKLVNPPEAQLVRGDDGLFRLQGGAPAPVDPNVRVAGGYLEGSNVNVVDQMVQMISLA
                     RQFEMQTRMLSTAEQNDRSAARVIGGQG"
     gene            complement(2893..4245)
                     /gene="flgE"
                     /locus_tag="PKN25_11520"
     CDS             complement(2893..4245)
                     /gene="flgE"
                     /locus_tag="PKN25_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNV91570.1"
                     /translation="MSFQQGLSGLSVSSKALDIISNNVANTNTIGFKTGSAIFSDVFA
                     ASLTGSISGKQVGVGATLGAVRQTFTQGNLTTTNNPLDLAINGDGFFVVSRENGPDVY
                     TRNGQFELDKEGFIRTPTGERLKGFQSPVTPGALPTPIGGLKDVQVPIVGSQPMPTSR
                     VEIGGNLDANDLSAAQRYPQLFGSTFTFPLPAGENWQDARTYNFSTSIKAYDSLGKSH
                     ELTYYFAKVTPTPNDPVNTNENTWKVFTSVDGGYPLGVGTDGSIEDPSVIWSLTFNEK
                     GTVNTPIVGPTAANPLAIPASYTPGADRIRFHVDFSDMRQIGGSYLITELTQDGYTGG
                     ELAGVSVGRDGIVTGRYTNGETRELAQLALQTFRNPNALLSIGNNFWEATTESGLTAP
                     SKAGEGVAGVVSAGMIEDANVELTNELVQMIVQQRNYQANAQSIKAQDQVLQTLVNLR
                     "
     gene            complement(4261..4935)
                     /locus_tag="PKN25_11525"
     CDS             complement(4261..4935)
                     /locus_tag="PKN25_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HNV91571.1"
                     /translation="MSSVTNNQDIAASVLAKINTREESKASASGDMNSRFLTLLTTQL
                     KNQDPLNPMENAEVTSQLAQMSTVDGIERLNKMFQSFVDGQSAYDSMQAAALVGRGVL
                     VEGKKLTLTEAGAVGGFELDAAADSVVLSIKDAAGLEIKKIELADVKAGSQNFIWDGT
                     ASDGSRAVDGAYTVELSALAEGKKVTGRTLEFGPVTSVIRGASGTDFQVGSLGIFKYD
                     DIKQIL"
     gene            complement(4986..5390)
                     /gene="flgC"
                     /locus_tag="PKN25_11530"
     CDS             complement(4986..5390)
                     /gene="flgC"
                     /locus_tag="PKN25_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNV91572.1"
                     /translation="MSMLNVFQIAGSALNAQSLRLNATASNLANADSVTSEDGQPYRA
                     KQVVFAARPVAGPAAVGVQVTQVVESAAPLRLVFEPNNPAANPDGYVEMPNVNVVEEM
                     VNMISASRSYQNNAEVMSTARTLLQRTLQIGQ"
     gene            complement(5402..5806)
                     /gene="flgB"
                     /locus_tag="PKN25_11535"
     CDS             complement(5402..5806)
                     /gene="flgB"
                     /locus_tag="PKN25_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNV91573.1"
                     /translation="MKTLFDRQIQFHQTALNLQAHRQQLIASNIANADTPHYKARDVD
                     FRAALQGALNASREGVQMATTQAGHIGSAPAPALESLTGYRSELQSAVDGNTVNMDVE
                     RAAFAENALHYEASVTFINGMLRSMNTAITGQ"
     gene            6003..6731
                     /gene="flgA"
                     /locus_tag="PKN25_11540"
     CDS             6003..6731
                     /gene="flgA"
                     /locus_tag="PKN25_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNV91574.1"
                     /translation="MTTPLHRRLAAGRLRGPAAALCAALVLLPSPAAIAQHAEGAVEA
                     RVRQFLQRQVAGLPGEVSIELAPLDPNSQLPACAALEPFLPAGTRAWGRFSVGVRCDS
                     PVTWTAYLQARVAVVADYLIAARPLRAGQVLGPADLGQRRGDLTALPDNLLTDPTQAS
                     GHHTRIAVAAGSPLRGDMLRVPHAVRQGQTVSVLGVGAGFRVASEGRAMNNAAPGEKV
                     RVRLADGQVVTGTAQAGGAVALEF"
     gene            6864..7163
                     /gene="flgM"
                     /locus_tag="PKN25_11545"
     CDS             6864..7163
                     /gene="flgM"
                     /locus_tag="PKN25_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNV91575.1"
                     /translation="MKIESSGKAIAPSTAAEARPRSPASPGATVSAGSEKVQLSSLSS
                     SLAKAEASIAQTPVVDAQRVEEIKQAIANGEFRIDAGRIADGLIDSVREMLAGQR"
     gene            7178..7669
                     /locus_tag="PKN25_11550"
     CDS             7178..7669
                     /locus_tag="PKN25_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNV91576.1"
                     /translation="MNRPAVPPSSADRQRLALLIEAEAIQLGEFVELLAREEALLIAG
                     EVDALVVLCQDKNERYRRLQRLSDDRSLLLGRFGQAVSDTVIRDWLAGLPRVLARWDE
                     VLGLARTTRERNALNGKLITERMAHNQAALSLLLSAADQPPLYDAEGHTRAIGGGRML
                     GSA"
     gene            7734..9722
                     /locus_tag="PKN25_11555"
     CDS             7734..9722
                     /locus_tag="PKN25_11555"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNV91577.1"
                     /translation="MAGTQGTPKQYDESSFRVLKGLEPVRERPGMYTRTDSPAHIIQE
                     VIDNAADEALGGFAKKIHVTLHVDGSVTVADDGRGIPVGLHPEEGVPVVVLAYTRLHA
                     GGKFDKREGNSAYAFSGGLHGVGVSVTNALSTRIEVEVRREGKIHRIDFSDGGETIGP
                     VRVEGDCGRQTGTRVRVWPDGKYFESPRVPMNELERLLRSKAVLLSGVAVRLDIEQAN
                     GPALTKTWSYPDGLAGYLKELAGDVEPVAPIFTAEKYAAKDDPTFAAGEGAAWALAWF
                     ESPVPSESYVNLIPTVNGGTHESGLRAGVFEAMKSFIDHHTLLPRGVKLQQEDVCGRM
                     SFVLSARLLDPQFQGQVKEKLNSREAVKLVSSQLRDPFEIWLNNHVEAGRAIAELSIK
                     QALARQKSAQKVEKKKTSGVAVLPGKLSDCESEDLADNELFLVEGDSAGGSAKMARNK
                     ETQAILPLRGKVQNAWEIDPDRLFANAEIHDIAVALGVDAHTADSPIDLSGLRYGKVV
                     IMSDADVDGAHIQTLLLTLFFRHFPKLIERGHVYVAQPPLYRIDVPAQGKKRPPRRLY
                     ALDEGELAAMRERLEKEGFKPDAIEIGRFKGLGEMNPDQLRETTMDPATRRVLPVKVR
                     SGALQDTLKMFTLLMGKGEASGRRAWMEEKGDSVEADV"
     gene            9811..10062
                     /locus_tag="PKN25_11560"
     CDS             9811..10062
                     /locus_tag="PKN25_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNV91578.1"
                     /translation="MHTISAAEANRQFSSLLRQVSQGETITVLSHGRPVATISPATEA
                     GESGHDRANARARLLARLNREPAAGERSWNRDELYDRDQ"
     gene            10059..10511
                     /locus_tag="PKN25_11565"
     CDS             10059..10511
                     /locus_tag="PKN25_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV91579.1"
                     /translation="MKIALDTNILAYAEGAGDDARCAAARALIEDLAASEVLIPAQAL
                     GELHRVLTGKLRRDAETVRGALLDWSDAYEVADSTWPSFQAAIDLACDHRMQIWDGLV
                     LSVAAENRCRLLLSEDLQHGFTWRGVTVINPFEHVAHPLLLAACNSSS"
     gene            10508..>10921
                     /locus_tag="PKN25_11570"
     CDS             10508..>10921
                     /locus_tag="PKN25_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNV91580.1"
                     /translation="MSTDTPDLFDAPGVPESVAPSTPAGAAPSAASTTRGAAPATASD
                     ADIPPPPAPPADPEPEPEDDGTLALDRYAERAYLAYAMSVVKSRALPQVEDGLKPVQR
                     RILFAMNEMRLSSTSKHVKSARVVGDVIGKYHPHGD"
BASE COUNT         1701 a         3692 c         3745 g         1783 t
ORIGIN      
        1 gtgccgccgc gcaggctctt ggcgttgccg atcgaggaca cggtgacgtc gatcgcctgc
       61 ccgggacggg cgaaggccgg caggttggcg gtgaccatca ccgcggccac gttcttcaac
      121 tgcaggttgg tcccgggcgg caggctcacg ccgaggttgc cgagcatgtt gacgatgctc
      181 tgcacggtga agggggtctg cgtggtctgg tcgcccgaac cgtccaggcc gacgaccagc
      241 ccgtagccga cgagctggtt gtcgcgcacg ccggccaccg aggcgaggtc cttgatgcgc
      301 tcggccgcgg cgtacgcctg cgccggatgc aagatcagca cggcgaggga gacgaccgcg
      361 gcgcgcagca gaagggaggc gagacaggac atggcgcggc cctcagaacg gcatgatcgc
      421 cacgaagaag cgctgcagcc agcccatggt gttggactcg tcgatgaagc cgctgccgcg
      481 gtactcgatg cgcgcatccg ccacctgggt cgactgcacg gtgttggcgg tggtgacggt
      541 gttcgggttg atcacgcccg agaagcggat gaactcgttg ccctggttga tcgccaccat
      601 cttctcgccc gacaccagca ggttgccgtt ggggtagacg tcgatcacgg tgaccgtgat
      661 cgtgccgttg aagacgttgt tggccgcggc cgcgcccttg ccgttgaagt cggactcggc
      721 atcggcctcg agctccaggc cgttcaggcc gctcaagggc aggcggttga tggggccgat
      781 gccggcgctc atgttgccgt tgcgggtggc gttggcgttg gcgctcttct ccgcggtgtt
      841 gcgctcgacc aggttgatcg tgatcgtgtc gccgatgcgg cgcgcgcgcc ggtcctcgaa
      901 cagcggacgt gcctgcacgg tctggtagat cgcgccattg gcgggcagcg cggtcgccat
      961 cgcctcgggg cgcacgctca tcggctgatg caccgcagac ggcggggtgg tctggatcgc
     1021 ggcgcagccc gacagcagcg cgagcaggcc gacggcgatc gaatgcctgg cgagcttcat
     1081 cgtgcgcctc acagctgggt cagccggccg agcatctggt cggaggtcga gatcgcgcgc
     1141 gagttgagct cgtaggcgcg ctgggtcacg atcatgctca cgagctcctc ggcgacgttg
     1201 acgttggaga cctcgacata gccctggttg agcacgcccg cgccgttggt gcccggctgg
     1261 gtgggcgtgg ccacgccgct cgaggcggtc tcgtagaaca ggttctcgcc cgcggcctgc
     1321 aggccgcccg agttgatgaa ggtggcgagc tggatggcgc cgatttccac cggcgcggtc
     1381 tgcccggcct gcagcacgct gaccgtgccg tccttgccga tggtgatcga gagcgcgtcg
     1441 gcggggatgt tgatgttgtc ggccagggtg taaccgctgg cggtgacgac gttgccctga
     1501 ctgtcgagct ggaaagcgcc gtcacgggtg taggcggtgg tgccatcggg catggcgacc
     1561 tggaaaaagc cgttgccctg caccgcgacg tcgagcgagc caccggtctg ctgcaatgtg
     1621 ccctgggtga agatgcgctc ggtcgccacc ggcttgacgc cggtaccgat ctgcaggccg
     1681 ctggagatct gcgtctgctg ggtcgactgt gcgccaggct ggcggatggt ctggtagagc
     1741 aggtcctcga agatcgcacg ctgacgcttg aagccgttcg tggagacgtt ggccaggttg
     1801 ttggagatca cgtccagcga ggtttgctgg gcatccagcc cggtacgggc ggtccacagg
     1861 gagcggatca tgatgggttc ctttgccttg cgttgccggg gcggcaggat ttgccgtgcg
     1921 gggttggaat acccagctga aattgccgcc gtgctgccgc tttttcgatg ggatcagtct
     1981 aggccggtgc cgtcaccgac catcctgcga taagcaacgc aaaaaccgtg ctaatcgaag
     2041 cggccgggtg cgcaaaaaag gccgggcgcc ccacgctacc cggcccgcgc agcccggctc
     2101 ggcggggagc gcgcctcgat cagccctggc cgccgatgac ccgcgcggcc gagcggtcgt
     2161 tctgctcggc ggtcgacagc atgcgggtct gcatctcgaa ctgacgggcg agcgagatca
     2221 tctgcaccat ctggtcgacc acgttgacgt tgctgccctc gagatagccc ccggcgacgc
     2281 gcacattggg atcgaccggc gcgggcgccc cgccctgcag gcggaagagg ccgtcgtcgc
     2341 cacgcaccag ctgcgcctcg ggcggattca ccagcttgag ctgcgcgacc acgttgacca
     2401 cacccggctg atcgggctgc gaggccgaga ccgaaccgtc ggcaccgatg gtgatctcgt
     2461 tgtcgggcgg gatcgtgacc ggcccgccgt cgcccagcac cggcctgccg tcgcgcgtct
     2521 gcaggacgcc gttggcgctc accgcgaggc tgccgttgcg ggtgtaggcc tcgctgccgt
     2581 ccggcatctg caccgccagc cagcctttgc cggcgagcgc gacgtcgaag ggccggccgg
     2641 tgaactgcag cggcccctcg gtgaagtcgt tggcgacgct ggcgtcgacc gtgaaggcgc
     2701 gcgtgcgcag cgcctcggtc tgcacctcga ccgcgcgcag cttgtgcaac tctgcgcgga
     2761 aacccgtggc gtcgacgttg gcgaggttgt gcgcgaccgc cgcctgctgg tccatggtgc
     2821 ccttggcgcc ggtcatcgcg gtgtagatca ggcggtccat cgctggcctc tcgtgtcagg
     2881 cgggtttcag gatcagcgca ggttcaccag ggtctgcagg acctggtcct gcgccttgat
     2941 ggattgcgcg ttggcctggt agttgcgctg ctgcacgatc atctgcacca gctcgttggt
     3001 gagctccacg ttggcgtcct cgatcatgcc cgccgagacc acgcccgcga ccccttcgcc
     3061 ggccttggac ggcgcagtga ggccggattc ggtggtcgct tcccagaagt tgttgccgat
     3121 cgacagcagc gcgttcggat tgcggaaggt ctgcagcgcg agctgggcga gttcgcgcgt
     3181 ctcgccattg gtgtagcgac cggtcacgat gccgtcccgc cccacgctga ctccggcgag
     3241 ctcgccgccg gtgtagccgt cctgggtgag ctcggtgatc aggtaggagc caccgatctg
     3301 gcgcatgtcg ctgaaatcga cgtggaaacg gatgcggtcc gcacccggcg tgtaggaagc
     3361 cgggatcgcc agcggattcg cagccgtcgg cccgacgatg ggcgtgttca ccgtaccctt
     3421 ctcgttgaag gtcaggctcc agatcacgct cgggtcctcg atcgagccat cggtcccgac
     3481 ccccaaggga tagccgccgt ccacgctggt gaagaccttc caggtgttct cgttcgtgtt
     3541 gaccggatcg ttcggggtcg gggtcacctt ggcgaagtag taggtgagct cgtgcgactt
     3601 cccgaggctg tcgtacgcct tgatcgaggt cgagaaattg taggtgcgcg cgtcctgcca
     3661 gttctcgccc gcagggagcg gaaacgtgaa ggtcgagccg aacagctgtg ggtagcgctg
     3721 ggctgcgctc aggtcgttcg catcgaggtt tccgccgatc tccacccggc tggtcggcat
     3781 gggctgggaa ccgacgatcg gcacctggac gtccttgagg ccgccgatcg gcgtgggcag
     3841 ggcgcccggg gtgaccggac tctggaagcc cttgagcctt tcgccggtgg gggtgcggat
     3901 gaaaccctcc ttgtcgagct cgaactggcc gttgcgggta tacacgtccg ggccgttctc
     3961 gcggctgacc acgaagaagc cgtcgccgtt gatcgccagg tcgagcgggt tgttggtggt
     4021 ggtgaggttg ccctgggtga aggtctgccg caccgcaccg agcgtggcgc ccacacccac
     4081 ctgcttgccc gagatcgagc cggtcagcga ggccgcaaag acgtccgaga agatcgcgga
     4141 gccggtcttg aagccgatcg tgttggtgtt ggcgacgttg ttggagatga tgtccagggc
     4201 cttggaggaa acgctcaggc cgctcaaacc ttgctggaaa gacatgatcg gggctccgga
     4261 tcagagaatc tgcttgatgt cgtcgtactt gaagatgccg agcgagccga cctggaagtc
     4321 ggtacccgag gcgccgcgga tcacgctggt gaccgggccg aactcgagcg tccgacccgt
     4381 gaccttcttg ccctcggcga gcgcgctcag ctcgaccgtg taggcgccgt cgacggcacg
     4441 cgaaccgtcg ctggcggtac cgtcccagat gaagttctgg ctgcccgcct tgacgtccgc
     4501 gagctcgatc ttcttgatct cgaggccggc ggcgtccttg atcgacagca ccacgctgtc
     4561 ggccgcggca tcgagctcga aaccgcctac cgcgccggcc tcggtgaggg tcagcttctt
     4621 cccctccacc agcacgccgc gcccgacgag cgcggcagcc tgcatcgagt cgtaggcgct
     4681 ctggccatcg acgaagctct ggaacatctt gttcaggcgc tcgatgccgt cgaccgtgct
     4741 catctgtgcg agctgcgagg tgacctccgc attctccatc gggttgagcg gatcctgatt
     4801 cttcagctgg gtggtgagca gggtgaggaa gcgcgagttc atgtcgccgg aggccgaagc
     4861 cttcgactct tcgcgcgtgt tgatcttggc gagcacgctc gccgcgatgt cctggttgtt
     4921 cgttacgctg ctcattcctc tctcctgctg tcggcgtttt ccgcgtcacg gcgcctgcgg
     4981 ggtgcttact ggccgatctg caaggtacgc tgaagcaagg ttcgtgccgt gctcatcacc
     5041 tcggcgttgt tctggtagga acgcgaggcg gagatcatgt tcaccatttc ctcgaccacg
     5101 ttgacgttgg gcatctcgac gtagccgtcg gggttggcgg cagggttgtt gggctcgaag
     5161 accagccgca ggggcgcggc gctctcgacc acctgggtca cctgcacgcc gaccgccgcc
     5221 gggccggcga ccgggcgcgc ggcgaacacc acctgcttgg cgcggtaggg ctggccgtcc
     5281 tcggaggtga cgctgtcggc gttggcaagg ttggaggcgg tggcattgag gcgcagcgac
     5341 tgcgcattga gcgccgagcc ggcgatctgg aagacgttca gcatgctcat ggcggctctc
     5401 cttactggcc ggtgatcgcg gtgttcatcg agcgcagcat gccgttgatg aaggtcacgc
     5461 tggcctcgta gtgcaacgcg ttctcggcga aggcggcgcg ctcgacgtcc atgttcaccg
     5521 tgttgccgtc caccgcggac tgcagctcgc tgcggtagcc ggtcaggctt tcgagcgcag
     5581 gcgcgggtgc gctgccgatg tggcccgcct gcgtggtcgc catctgcaca ccctcccgcg
     5641 aggcattgag cgcgccctgc agcgcggcgc ggaaatccac gtcgcgcgcc ttgtagtgcg
     5701 gcgtgtcggc gttggcgatg ttcgaggcga tcagctgctg gcggtgcgcc tgcaggttca
     5761 gcgcggtctg gtggaactgg atctgccggt cgaaaagggt cttcatcgct gtgctcccgg
     5821 gacggtgcgc ccggacgttc cggacgcagt ttccatgcca gcgatggtag gcgcgggccg
     5881 gcaagaccct ggcgccgata aggggggcaa agcccggcca atttcgccgc cacccgccgg
     5941 caaggattgc cggcggacgc tgccggttgc caggccggcg ggggtcatgg tgcaatcccg
     6001 ccatgacgac tcccctccac cgcaggctcg ccgcagggcg actgcgcggc ccggccgcag
     6061 cgctgtgcgc ggccctcgtg ctgcttccct cccccgccgc gatcgcccag cacgccgagg
     6121 gggcggtcga ggcccgcgtg cgccagttcc tgcagcgcca ggtcgccggc ctgcccggcg
     6181 aggtgtcgat cgagctcgcg ccgctcgatc ccaacagcca gttgcccgcc tgcgccgcgc
     6241 tcgaaccctt cctgcccgcg ggcacgcgcg cgtggggacg cttcagcgtc ggtgtgcgct
     6301 gcgactcacc ggtgacgtgg accgcctacc tgcaggcgcg cgtcgccgtg gtcgccgact
     6361 acctgatcgc cgcccgcccg ctgcgcgccg gccaggtgct cggccccgcc gacctcgggc
     6421 agcgcagggg cgacctcacc gcgctccccg acaatctcct caccgacccc acccaggcca
     6481 gcggccacca cacccgcatc gcggtcgccg ccggcagccc cttgcgcggc gacatgctgc
     6541 gcgtacccca cgcggtgcgc cagggccaga ccgtgagcgt gctcggcgtg ggcgcgggct
     6601 tccgggtggc gagcgaaggc cgtgcgatga acaacgccgc cccgggtgag aaggtgaggg
     6661 tgcgcctggc cgacggccag gtcgtcaccg ggaccgcaca ggcgggcggc gcagtggcgc
     6721 tggagttctg aggcggctgc gtgccgaacg gagacggccg cgagcggatg gccgagcgtg
     6781 aaagatttca tgctttaatc gctcaagttc cacgccatcg cgccgaagag aacgtcagag
     6841 tcactctgta tgggaactgc gccatgaaaa tcgaaagctc gggcaaggcg attgccccct
     6901 ccaccgcagc ggaggcccgc ccacgcagcc ccgcgagccc cggcgccacg gtctccgccg
     6961 gcagcgaaaa ggtccagctc tcctcgctgt cgtccagcct cgccaaggcc gaggcgagca
     7021 tcgcccagac cccggtcgtc gacgcgcagc gcgtcgagga gatcaagcag gcgatcgcca
     7081 acggcgaatt caggatcgac gccggacgca tcgccgacgg gctgatcgac agcgtgcgcg
     7141 agatgctcgc aggccagcgc tgaacgcttc accgcgcatg aaccgtcctg ccgtcccgcc
     7201 ctcctccgcc gaccgccagc gcctcgcgct gctgatcgaa gcggaagcga tccagctcgg
     7261 cgagttcgtc gagctgctcg cgcgcgagga ggcgctgctg atcgccgggg aggtcgatgc
     7321 actggtggtg ctctgccagg acaagaacga acgctaccgc cgcctgcaac ggctctccga
     7381 cgaccgcagc ctgctgctcg gccgcttcgg ccaggcggtc tccgacaccg tgatccgcga
     7441 ctggctcgcc ggcttgccgc gcgtgctggc gcgctgggac gaggtcctcg gcttggcccg
     7501 cacgacgcgc gagcgcaacg ccctcaacgg caagctcatc accgagcgca tggcgcacaa
     7561 tcaggccgcg ctcagcctgc tgctctcggc cgccgaccag ccgccgctct acgacgccga
     7621 aggccatacc cgcgccattg gcggcgggcg catgctcggc agcgcctgaa catacataca
     7681 gcccttcgct ctgctatcct tgcgcccttc gcaagttcga acgggcagtc agcatggccg
     7741 gcacgcaagg cactccaaag cagtacgacg aatcctcctt ccgcgtcctg aaagggctcg
     7801 agccggtgcg cgaacgcccc ggcatgtaca cccgcaccga cagcccggcg cacatcatcc
     7861 aggaggtgat cgacaacgcc gccgacgagg cgctcggcgg cttcgcgaaa aagatccacg
     7921 tcacgctcca tgtcgacggc tcggtcaccg tggccgacga tggccgcggc atcccggtcg
     7981 gactgcaccc cgaggaaggc gtcccggtgg tggtgctggc ctacacccgg ctgcacgccg
     8041 gcggcaagtt cgacaagcgc gagggcaact cggcctatgc cttctcgggc ggcctgcacg
     8101 gcgtcggcgt gtcggtgacc aacgcgctgt cgacccggat cgaggtcgag gtcaggcgcg
     8161 agggcaagat ccaccgcatc gacttctccg acggcggcga gacgatcggc ccggtgcgcg
     8221 tcgagggcga ctgcggacgc cagaccggca cccgggtgcg ggtgtggccg gacggcaagt
     8281 acttcgaatc tccgcgcgtg ccgatgaacg agctcgagcg cctgctgcgc tcgaaagcgg
     8341 tgctgctgtc gggagtcgcg gtgcggctcg acatcgagca ggcgaacggc ccggcgctga
     8401 ccaagacgtg gtcctacccc gacggcctcg ccggttatct gaaagagctc gcgggcgatg
     8461 tcgagccggt ggcgccgatc ttcaccgccg agaaatacgc cgccaaggac gatccgacct
     8521 tcgccgccgg tgaaggcgcg gcgtgggcgc tggcatggtt cgaatccccg gtgccgagcg
     8581 agtcctacgt caacctgatc ccgaccgtga acggcggcac ccacgaatcc ggcctgcgcg
     8641 ccggcgtgtt cgaggcgatg aagtccttca tcgaccacca caccctgctg ccgcgcggcg
     8701 tgaagctgca gcaggaggac gtgtgcgggc gcatgagctt cgtgctctcg gcgcgcctgc
     8761 tcgacccgca gttccagggc caggtgaagg aaaagctcaa ctcgcgcgag gcggtcaagc
     8821 tcgtctccag ccagctgcgc gaccccttcg agatctggct caacaaccac gtcgaggccg
     8881 gccgcgcgat cgccgagctt tccatcaagc aggcgcttgc gcgccagaag agcgcgcaga
     8941 aggtcgagaa gaagaagacc tcgggcgtgg cggtgctgcc cggcaagctg tcggactgcg
     9001 agtccgagga cctcgccgac aacgagctct tcctggtcga gggcgactcc gccggcggct
     9061 cggccaagat ggcgcgcaac aaggagaccc aggccatcct gcccttgcgc ggcaaggtgc
     9121 agaacgcctg ggagatcgac ccggatcgcc tcttcgccaa cgccgagatc cacgacatcg
     9181 cggtggccct cggcgtggac gcgcacaccg ccgacagccc gatcgacctc tccgggctgc
     9241 gctacggcaa ggtcgtcatc atgtccgacg ccgacgtcga cggcgcccac atccagaccc
     9301 tgctgctgac gctgttcttc cgccacttcc ccaagctcat cgagcgcggc cacgtgtatg
     9361 tggcgcagcc gcccctgtac cgcatcgatg tcccggcaca aggcaagaag cgcccgccgc
     9421 gccgcctcta cgcgctcgac gagggcgagc tcgccgcgat gcgcgagcgc ctggagaagg
     9481 aaggcttcaa gcccgacgcg atcgagatcg gccgcttcaa gggcctgggc gagatgaacc
     9541 ccgaccagct gcgcgagacc accatggacc cggccacccg tcgggtgctg ccggtgaagg
     9601 tgcgcagcgg cgcgctgcag gacacgctga agatgttcac cctgctgatg ggcaagggcg
     9661 aagcctcggg gcggcgtgcg tggatggaag aaaagggcga cagcgtcgag gcggatgtct
     9721 gagtccccca aggcaaatcg aattgcccga cgccatcacc cggaataaag tagtgatatt
     9781 cactacaccg atgcaagcta cggacctgcc atgcacacca tttccgccgc cgaagcgaat
     9841 cgacagttct ccagcctgct ccgccaggtc agccagggcg agaccatcac ggtcctgtcc
     9901 cacggccgtc ccgtcgccac gatcagcccc gccacagaag cgggcgaatc cggtcacgac
     9961 cgtgccaacg cacgcgcccg tctgctcgcc cgcctcaatc gggaacctgc cgcaggcgaa
    10021 cgaagctgga accgcgacga actctacgac cgcgaccaat gaagatcgcg ctggacacca
    10081 acatcctcgc ctacgcagag ggcgccggag acgacgcgcg ttgtgcagcg gctcgtgcgc
    10141 tgatcgagga tctcgcggcg agcgaggtgc tgatcccggc gcaggccttg ggtgagttgc
    10201 accgggtcct caccggcaag ctcaggcgcg acgccgagac agtgcgcggc gccctgctcg
    10261 actggtccga tgcctacgag gtggccgatt cgacctggcc gagcttccag gccgccatcg
    10321 acctggcctg cgatcaccgc atgcagatct gggatggcct ggtcctttcg gtggctgcgg
    10381 aaaaccgttg ccgcctgctg ctgagcgaag acctgcagca cggcttcacc tggcgcggcg
    10441 tcaccgttat caatcctttc gagcatgtcg cgcacccgct actgctcgca gcatgcaatt
    10501 cgagctcatg agcacagaca ctcccgacct cttcgacgcg cctggcgtgc ccgaatccgt
    10561 cgccccctcg accccggccg gcgcagcccc gagtgctgcc tcgacaactc ggggcgcagc
    10621 gcctgcgacc gcctccgacg cagacatccc cccgccccct gccccgcccg ccgacccgga
    10681 acccgagccc gaggacgacg gcacgctggc gctcgaccgc tacgccgagc gcgcctatct
    10741 cgcctacgcg atgagcgtgg tgaagtcgcg cgcgctgccg caggtcgagg acggcctgaa
    10801 gccggtgcag cgccgcatcc tgttcgcgat gaacgagatg cggctgtcgt ccacctccaa
    10861 gcacgtgaag tcggcgcgcg tggtcggcga cgtgatcggc aagtaccacc cgcacggcga
    10921 c
//
LOCUS       DAOQBS010000106        10774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_5368_length_10774_cov_15.564885, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000106 DAOQBS010000000
VERSION     DAOQBS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10774
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5368_length_10774_cov_15.564885"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKN25_11575"
     CDS             complement(<1..306)
                     /locus_tag="PKN25_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV91581.1"
                     /translation="MSHLDLSRIDLAFGSHLVVRQLSLQLEKGRIGCLLGPSGCGKTT
                     VLRCIAGFERLAAGEIRLDGEVVSTPRHMLPPERRRIGMVFQDYALFPHLSVAYNIGF
                     "
     gene            460..2100
                     /locus_tag="PKN25_11580"
     CDS             460..2100
                     /locus_tag="PKN25_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNV91582.1"
                     /translation="MRIRFSSLTVLAVVVATLIAAPVLSVFSNVLAGDTGATWSHLAD
                     TVLGEFVFNTVVLCIGVGLGVSSIGVTSAWLTTMLDFPGRRFLEWALVLPLAMPAYVM
                     AYVYTDFLQFVGPVQGWLRETFGWGRGDYWFPDVRTVGGAVAMFMFVLYPYVYMIART
                     AFLERAGGMLEAGRTLGLGPWGSFFRVSLPLARPAVVAGTALALMETLADFGTVSYFG
                     VQTFTTGIYRAWFSLGDRIAAAQLSAALLAFVVLVLTLERMSRGRARFNNTSRQVAMP
                     VRLRLPLALGVLAAFACFMPLLLGFLLPAALLLRMAFLEGDAQFGARFVELAHNSFVL
                     AAITAVVAVLLAVLLAYAARLARSRLPQVLNRIVGLGYAVPGSVIAVGVLIPLTRVDR
                     WLAQGWEALWGTNPGLLLTGGIAALIYAYLARFLAVALQTVESSLGKITPNMDDASRS
                     LGFGKWATLRRVHMPMLRGSLLTAGLLVFVDVMKELPATLVIRPFNFDTLATQAHTLA
                     ADERLAEASTAALIIVAVGLLPMFWISRQILRVRRREG"
     gene            2310..4442
                     /locus_tag="PKN25_11585"
     CDS             2310..4442
                     /locus_tag="PKN25_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HNV91583.1"
                     /translation="MATSVLHRFRRPLQATGLLGVLFVLSVFMPPVRLFADGRDYLPL
                     HSGLEIFSLVVAGMVFALGWNLRRSARGARLVWLGAACLAVGLLDFAHLMSFPGMPPF
                     FTPNSADKAIAFWLMARLLGVLGLLAHAVLPERDGGGSAAAWAVLAAIGVAFGWSWLV
                     LAHPQVLPATFIEGQGVTPLKSVLEGGLVLLHLAAAILLARRALADGESAAGWLAAAA
                     ATMALAELHFARYAAVADLYNVLGHAYKVAAFAMIYRAVFVSGVRAPQRALAAERALL
                     RGLIDSVPDLIAFRDREGRYLGCNKAFAEAYGIREAALVGRSDGELFAGRLPLAGAGQ
                     DTVSCAENHEEWLEAADGTLRLLDTLRTPYFDLDGVQLGEIRVSRDFTERRRVREQIA
                     ERERRLMLALEGAELGLWDWDVPSGRANFSPMWCRMLGYAAEELPANVSSWESLVHPD
                     DWYDIRDSLEPHLRGTKDAYRAEYRLKHKSGHWVWVLAAGRVLDRGAGGEALRMVGIH
                     QDISQRKAMEESLLQLATSDPLTGLWNRRHFVEVVRGELGRVRRNQAPAALLLMDLDF
                     FKRINDTHGHAAGDEVLRHFTATVSAQLREADVFARLGGEEFAVLLPSIDALGAVRAA
                     DRLRRVVADSPASVDSGALHYSVSIGATMLDAADEGYEAAYARADEALYAAKHAGRNR
                     VVLAPASAGAPCGEPVSEGAAAGAADQR"
     gene            complement(4433..5446)
                     /locus_tag="PKN25_11590"
     CDS             complement(4433..5446)
                     /locus_tag="PKN25_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV91584.1"
                     /translation="MSKKVLIASIIAAFGLTGTVTAAELNVYSARHYQTDEELYGNFT
                     KQTGIKINRIEAKEDELLERIRNEGANSPADIFVTVDASRLAKADELGIFRPVKSAAL
                     EARIPAHLRAPNWFSYSTRARVIVYNPDMVKAEQVQTYEQLADPALKGQVCTRSGSHP
                     YNLSLGAAMIKHNGAEATENWARGIVANFARAPKGGDTDQIRAVAAGECGVAIANSYY
                     LARLMNSDKREDQAVVAKIKAVWPNQATWGTHINVSGAGMLEHAPNKEAAVKFLEYLA
                     SDQAQEYFANGNNEWPAVPSVKVDNPALKKLGEFKADTLPIGELADTVAEAQRIFDRA
                     GYR"
     gene            5564..5923
                     /locus_tag="PKN25_11595"
     CDS             5564..5923
                     /locus_tag="PKN25_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HNV91585.1"
                     /translation="MNALLVGADRLGNIPDVLHNFGIRIAAHVSGRDTTHQRRSTPLP
                     SGIGLVILFTDFLGHNVMQRFREAAGKQGVQVVCCRRSTCALQQALDRQMGSRCDECP
                     RRCEKASGGRRRASSAC"
     gene            complement(5981..6532)
                     /locus_tag="PKN25_11600"
     CDS             complement(5981..6532)
                     /locus_tag="PKN25_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNV91586.1"
                     /translation="MFFRTSLPALLASFALALPAQGAEPAPAQREHAFQASSAFEEYT
                     TAAEQLVDEALSYLGIRYRFGGTSPATGFDCSGLVLNVFRNAVGLDLPRSASEMASLG
                     DKIGKQDLKPGDLVFFNTMRRTFSHVGIYLGDGKFVHAPSSGGKVRVENISATYWAKR
                     FNGARRLVDEESLGLSAQVMRTH"
     gene            complement(6621..7262)
                     /locus_tag="PKN25_11605"
     CDS             complement(6621..7262)
                     /locus_tag="PKN25_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNV91587.1"
                     /translation="MSWLSRLKNARGNQPPLSAEAERALEAWHALAESAHEQPHFESR
                     YVVLNTEATGLDLERDRLLAVGAIAVDGGLLHPQDAYHAQLEPLPGDALAGLLGFARN
                     SPLVVFNATFNRTMLERAFSRMLGFTPEFLWIDLYFLLPSLFPERLERPGRLADWMAA
                     FEIETFQRHHALGDAWAIAQLFLAAQSRALSLAVTSPRALAEHAQAYRQKIKV"
     gene            complement(7259..9160)
                     /locus_tag="PKN25_11610"
     CDS             complement(7259..9160)
                     /locus_tag="PKN25_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HNV91588.1"
                     /translation="MSNDAADLLVGASMDFLRNFSPFNRMEPGALAFLAERAVLAFHA
                     RGSEILTPESGVPSHFYIVQRGKIQARQTGQTTVTEYTSLTLGPGECFPIGAISARRP
                     STNAYVAVEDSFCFQISADDFLHLMQISPVFHLFCTQYIASLLSQSRQQLQSTFAQRA
                     AEQQTMTTALGKLIEKEAVFVTPDTPTRSALERMSELHLGCMVVVDAERRPVGILTQS
                     DLLPRVILAGFDLARPIGELMTANPHQLPASASAYDAALEMATHGVRHLLVVDSDSVL
                     LGVVSERDLFSLQRISLRQIRAGIDNAADIEALQRASKDIRQLALNLIAQGIGAEQLT
                     QFISALNDALTRRIIILARERHELVGIDFAWLAFGSEGRHEQTLSTDQDNGIIFRCAE
                     PKQLKDTRARLVEFARTVNEDLAACGFPLCKGNIMASNPELCLTLDEWKSRFGDWIRE
                     PDPQALLNASIFFDFRVLYGNERFGDQLRSALNKGAKGNSAFLRMMAANALKVMPPLG
                     RIRDFVVDEDGTIDMKKSGARLFVDVARILALRTGVESSSTVQRLRQGSTKIGGMSPE
                     EVSAIIDGFNFIQLLRLRSQHLETEHDSAEDNRIDPESLNELDRRILKEAFRQARKLQ
                     LRLKLDYQL"
     gene            complement(9235..10713)
                     /locus_tag="PKN25_11615"
     CDS             complement(9235..10713)
                     /locus_tag="PKN25_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNV91589.1"
                     /translation="MSALRKSATSPSLPADAGEPAIKRVRRARKAPAACKLFVLDTNV
                     LMHDPTSLYRFEEHDLYVPIMTLEELDNNKKGTSEVARNARQASRMLDEIVCASEDGI
                     AAGIALEGPSRGQATGRLHLQTEAIDIKLPPALPTARGDNQILAVVMFLVEKHPGREV
                     ILVSKDINMRIKARALGLAAQDYFNDKVLEDSDLLYTGTCELPADFWDSHARGMQSWK
                     EEGRSYYRVSGPLAAEMLVNEFVYQDGDNPLQAWVKEKEGRSALIETLTDYSHHKNNV
                     WGITARNREQNFALNLLMNPEIDFVTLLGQAGTGKTLLTLAAGLTQVLESKRYSEIIM
                     TRVTVPVGEDIGFLPGTEEEKMAPWMGALEDNLDVLNGTAGEGGDWGRAATRDLIRSR
                     IKIKSLNFMRGRTFLNKFLIIDEAQNLTPKQMKTLITRAGPGTKVVCMGNIAQIDTPY
                     LTEGSSGLTFVVDRFKGWAHSGHITLQRGERSRLADHAAEVL"
BASE COUNT         1499 a         3386 c         3962 g         1927 t
ORIGIN      
        1 gaagccgata ttgtacgcca ccgagagatg cgggaagagc gcatagtcct ggaacaccat
       61 gccgatgcgc cggcgctcgg gcggcagcat gtggcgcggc gtgctgacga cctcgccgtc
      121 gaggcggatc tcgccggccg ccagccgttc gaagccggcg atgcagcgca gcaccgtggt
      181 cttgccgcag ccggaaggcc cgagcaggca gccgatgcgg cccttctcga gctgcagtga
      241 gagctggcgg accacgaggt ggctgccgaa ggcgaggtcg atgcgggaaa ggtcgaggtg
      301 ggacatcgcg gtcgtcgagg gcctatgggc gtggggaatt attgcacccc ggtgttccaa
      361 atgcgatcaa ttataattcg ctcagttcgc gggggccgat ttccggcgcc ggggcgcaat
      421 catgggaacg gcatggattc gacggcaggg cagggcgcga tgcgcattcg gttttcttcg
      481 ctgacagtgc tggcggtcgt ggtggccacg ctgatcgcgg cgccggtgct gtcggtgttc
      541 tccaacgtgc tcgccggcga caccggggcg acctggtcgc acctggccga cacggtgctc
      601 ggcgagttcg tgttcaacac cgtggtgcta tgcatcggcg tcggcctggg ggtgtcgtcg
      661 atcggcgtga cctcggcctg gctgacgacg atgctggact ttcccgggcg gcgcttcctg
      721 gagtgggcgc tggtgctgcc gctggcgatg ccggcctatg tgatggccta cgtgtatacc
      781 gattttctcc agttcgtcgg cccggtgcag ggctggctgc gcgagacctt cggatggggg
      841 cgcggcgact actggttccc cgacgtgcgc accgtcggcg gcgcggtggc gatgttcatg
      901 ttcgtgctct acccgtatgt ctacatgatc gcgcgcaccg ccttcctgga gcgtgcgggc
      961 gggatgctcg aggccgggcg cacgctcggg ctgggtccgt ggggctcgtt cttccgcgtc
     1021 tccctgccgc tggcgcgccc ggcggtggtg gccggcaccg cgctggcgct gatggagacg
     1081 ctggccgact tcggcaccgt gtcctacttc ggcgtgcaga ccttcaccac cggcatctat
     1141 cgcgcctggt tctcgctcgg cgaccgcatc gccgccgcgc agctgtcggc cgcgctgctc
     1201 gccttcgtgg tcctggtgct gaccctggag cgcatgtcgc gcgggcgggc gcgcttcaac
     1261 aacacctcgc gccaggtggc gatgccggtg cggctgcgcc tgccgctggc gctcggtgtg
     1321 ctcgccgcgt tcgcctgctt catgccgctg ctgctcggct tcctgctgcc ggcggcgctg
     1381 ctgctgcgca tggccttcct cgagggcgat gcccagttcg gcgcgcgctt cgtcgagctg
     1441 gcgcacaaca gcttcgtgct cgcggcgatc accgcggtgg tggcggtgct gctcgcagtg
     1501 ctgctcgcct acgccgcacg cttggcgcgc tcgcgcctgc cgcaggtgct caaccgcatc
     1561 gtcggactgg gctacgcggt gcccggctcg gtgatcgcgg tgggcgtgct gatcccgctg
     1621 acccgggtgg atcgctggct ggcccagggc tgggaggcgt tgtgggggac gaaccccggg
     1681 ctgctgctca ccggcggcat cgccgcgctg atctacgcgt acctggcgcg cttcctcgca
     1741 gtggcgctgc agacggtcga gtccagcctg ggcaagatca cccccaacat ggacgacgcc
     1801 tcgcgcagcc tgggcttcgg caagtgggcg acgctgaggc gggtgcacat gccgatgctg
     1861 cgcggtagcc tgctcaccgc cggcctgctg gtgttcgtcg atgtcatgaa ggagctgccc
     1921 gccacgctgg tgatccgacc cttcaacttc gacaccctcg ccacccaggc gcacacgctt
     1981 gcggccgacg agcgcctggc cgaagcctcg accgcggcgc tgatcatcgt ggcggtgggc
     2041 ttgctgccga tgttctggat ttcgcgccag atcctgcgcg tgcgccgccg ggagggctga
     2101 gcgaggtgcc tgccgacgcg aaataatgca cgcggggtgc gcagccgcgg cggcggtcgc
     2161 gggtcgatca tgcataatta cgcgatgtcc ccgcgtcctt caatcttcgt cccgtgcagc
     2221 gctcggcaag ctgcccgggt cgtccatacc atgccttccg gctggggggg caaaccacgc
     2281 tcctgcggtg tgcccgaggc gcgttgatct tggcgacctc ggtgctgcac cgcttccgtc
     2341 gtccgctgca ggccaccggc ctgctcgggg tgctcttcgt cctcagcgtc ttcatgccgc
     2401 cggtgcgcct gttcgccgat gggcgcgatt acctcccgct gcattcaggc ctggagatct
     2461 tctcgctcgt ggtcgccggg atggtgttcg cgcttggctg gaacctgcgc cgcagcgcgc
     2521 gcggggcacg gctggtctgg ctcggcgcag cctgcctcgc ggtggggctg ctcgatttcg
     2581 cccacctgat gtcctttccg ggcatgccgc ccttcttcac gcccaacagc gcggacaagg
     2641 cgatcgcctt ctggctgatg gcgcgtctgc tcggtgtgct gggcctgctc gcccatgccg
     2701 tgctgccgga gcgcgatggc ggcggatccg ccgcggcctg ggccgtcctc gctgcgatcg
     2761 gcgtcgcctt cggctggagc tggctcgtcc tcgcgcaccc gcaggtcttg ccggcgacct
     2821 tcatcgaggg gcagggcgtc accccgttaa agagcgtgct ggagggtggg ctcgtgctgc
     2881 tgcaccttgc tgccgcgatc ttgctcgcgc gccgcgcgct ggcggacggc gaatccgccg
     2941 ctggctggct ggccgccgcc gcggcgacga tggcgctggc cgagctgcat ttcgcgcgtt
     3001 atgccgcggt cgccgacctt tacaacgtgc tcgggcacgc ctacaaggtg gcggcgttcg
     3061 cgatgatcta tcgcgcggtg ttcgtgtccg gcgtgcgtgc gccacagcgg gcgctggcgg
     3121 ccgagcgggc gctgctgcgc gggctgatcg attcggtgcc cgacctgatc gccttccgcg
     3181 atcgggaagg ccgttacctg ggctgcaaca aggccttcgc cgaggcctac gggatccgcg
     3241 aggcggccct ggtcgggcgc agcgacggcg agctgttcgc ggggcggctg ccgctcgccg
     3301 gggccggcca ggacaccgtg agctgcgcag agaaccacga ggagtggctg gaggcggcgg
     3361 acggaacgct acgtctgctc gacaccttgc gcacccccta tttcgacctc gacggcgtgc
     3421 agctcggcga gatccgcgtc agccgggact tcaccgaacg gcgccgggtg cgcgagcaga
     3481 tcgccgagcg cgaacgccgc ctgatgctcg cactcgaagg tgccgagctc ggtttgtggg
     3541 actgggacgt gccgagcggg cgagcgaatt tcagcccgat gtggtgcagg atgctcggct
     3601 atgcagcgga ggagctgccg gcgaatgtat cgagctggga gtccctggtg catccggacg
     3661 actggtacga catccgcgac tcgctcgagc cgcacctgcg cggcaccaag gatgcctatc
     3721 gcgcggaata ccggctcaag cacaagagcg ggcactgggt ctgggtgctc gcggcgggcc
     3781 gtgtactcga tcgcggcgcg ggcggggagg cgctgcgcat ggtcggcatc caccaggaca
     3841 tctcgcagcg caaggcgatg gaggagagcc tgctgcagct ggccaccagc gatccgctca
     3901 ccgggctatg gaatcgccgc cacttcgtcg aggtcgtgcg cggcgaactc ggacgcgtgc
     3961 gccgcaacca ggcgccggca gcgctgctgc tgatggacct cgacttcttc aagcgcatca
     4021 acgacaccca cggccatgcc gcgggtgacg aggtgctgcg ccatttcacc gcgacggtga
     4081 gcgcgcagtt gcgcgaggcc gacgtgttcg cacgcctggg cggggaggag ttcgcggtcc
     4141 tgcttcccag catcgacgcg ctcggcgcgg tgcgggcggc ggaccggctg cgcagggtgg
     4201 tggcggattc gccggccagc gtcgactcgg gggcgctgca ctattcggtc agcatcggcg
     4261 cgaccatgct ggacgcggcc gacgagggct acgaggccgc ctatgcgcgc gccgacgagg
     4321 ccttgtacgc ggccaagcac gccgggcgta accgcgtcgt gctggcgccg gcgagtgccg
     4381 gtgcgccctg cggcgagccc gtcagcgagg gggccgccgc aggggctgcg gatcagcggt
     4441 agccggcgcg gtcgaagatg cgctgggcct cggccaccgt gtcggcgagc tcgccgatcg
     4501 gcagggtgtc ggccttgaac tcgccgagct tcttcagcgc ggggttgtcc accttcacgc
     4561 tcggcaccgc cggccattcg ttgttgccgt tggcgaagta ttcctgcgcc tggtccgagg
     4621 cgagatactc gaggaacttg accgccgcct ccttgttcgg cgcgtgctcg agcatgccgg
     4681 cgccggacac gttgatgtgg gtgccccagg tcgcctggtt cggccacacc gccttgatct
     4741 tcgcgaccac ggcctggtct tcgcgcttgt ccgagttcat caggcgggcg aggtagtagc
     4801 tgttggcgat ggccacgccg cactcgcccg cggccacggc gcggatctgg tcggtgtcgc
     4861 cgcccttggg cgcgcgtgcg aagttggcga cgatgccccg ggcccagttc tcggtcgctt
     4921 ccgcgccgtt gtgcttgatc attgcagcgc cgagcgagag gttgtacggg tggctgcccg
     4981 agcgggtgca cacctggccc ttgagcgcgg ggtcggcgag ctgctcgtag gtctgcacct
     5041 gctcggcctt gaccatgtcc gggttgtaga cgatcacgcg tgcgcgggtc gagtaggaaa
     5101 accagttcgg tgcgcgcagg tgggcgggga tgcgggcctc gagcgcggcg gacttcaccg
     5161 gccggaagat gccgagttcg tcggccttcg ccaggcgcga ggcatcgacg gtgacgaaga
     5221 tgtcggccgg actgttggcg ccctcgttgc ggatgcgctc gagcagttcg tcttccttcg
     5281 cctcgatgcg gttgatcttg atcccggtct gcttggtgaa gttgccgtag agctcttcgt
     5341 ctgtctggta gtggcgggcg gagtagacgt tcagctcggc ggcggtgacg gtgccggtga
     5401 gaccgaaggc ggcgatgatc gaggcgatga ggactttctt gctcatggtg tgcgaacctt
     5461 ggtgtgtttg cggtgcgacg atactaacga taaaggttcg cagttgcaaa tggatcgcat
     5521 tcacttcatt attgttgaga aatgttctca acaaggagat tccatgaatg cgctgctcgt
     5581 cggtgccgac cgcctcggca acatcccgga tgttctgcac aacttcggca ttcgcatcgc
     5641 cgcccacgtc agcggccgcg acacgacgca ccagcgccgc agcacgccct tgccgagcgg
     5701 gatcgggctg gtgatcctgt tcaccgattt tctcggccac aacgtcatgc agcgcttccg
     5761 tgaggccgca ggcaagcagg gcgtgcaggt ggtgtgctgc cggcgctcca cctgcgcgct
     5821 gcagcaggcg ctcgatcggc agatgggttc gcgctgcgac gaatgcccgc ggcgctgcga
     5881 gaaggcatcc ggtgggcgcc ggcgggcgtc atctgcctgc tgaaaaagca agaggccccg
     5941 gcatgcgggg cctcttcctc ggcgtgttcc tcgccggcgc tcagtgcgtg cgcatcacct
     6001 gggcgctgag accgagcgat tcctcatcga ccagccggcg ggcgccgttg aagcgtttgg
     6061 cccagtacgt cgcggagatg ttctcgacgc gaaccttgcc gccgctcgag ggggcatgca
     6121 cgaacttgcc gtcgccgaga tagatgccga cgtgcgagaa ggtgcgtcgc atggtgttga
     6181 agaacaccag gtcgcccggc ttgaggtcct gcttgccgat cttgtcgccc aggctcgcca
     6241 tctcgctcgc gctccgcggc aggtcgagcc cgacggcatt acggaacacg ttgagcacga
     6301 gtccgctgca atcgaagccg gtcgccggcg aagtgcctcc gaagcgatag cggataccca
     6361 ggtaggagag cgcctcgtcg accagttgtt cggcggccgt ggtgtactcc tcgaacgccg
     6421 aagacgcctg gaaggcgtgc tcgcgctgcg ccggggccgg ctccgcgcct tgggcgggaa
     6481 gggcgagcgc gaaggaggcg agcagggcag gtagggatgt ccggaaaaac attcgcggaa
     6541 atatacgcat cgagctatcc ggtgtaaagg gaaatctgcg cctgtcggac gcattcgttc
     6601 cggttcttgt cggcctgctt tcagaccttg atcttttgcc ggtaggcctg ggcgtgctcg
     6661 gcaagcgcgc gcggactggt gaccgcgagc gagagtgcgc gcgactgcgc cgcgaggaag
     6721 agttgcgcga tcgcccaggc gtcgccgagc gcgtggtggc gctggaaggt ctcgatctcg
     6781 aaggcggcca tccagtcggc gagccgaccg gggcgttcca gccgctcggg aaacagcgag
     6841 ggcagcagga aatagaggtc gatccagagg aactctggcg tgaagccgag cattctcgag
     6901 aacgcgcgct cgagcatcgt gcggttgaag gtggcgttga acacgacaag cgggctgttg
     6961 cgggcgaagc cgagcagccc ggcgagcgca tcgccgggca agggctcgag ctgcgcgtga
     7021 taggcgtcct gcgggtgcag caggccgccg tcgacggcga tggcgccgac cgcgagcagg
     7081 cggtcgcgtt cgaggtcgag gccggtggcc tcggtgttga gcaccacgta gcgcgactcg
     7141 aaatgaggct gttcgtgcgc ggactcggcg agcgcatgcc aagcctcgag cgcgcgctcg
     7201 gcctcggcgc tcagcggcgg ctggttgccg cgggcgttct tcaggcgaga gagccagctc
     7261 atagttggta atcgagttta agccgcagct ggagcttgcg cgcctggcgg aaggcttcct
     7321 tgaggatgcg gcggtcgagt tcgttgagcg attcggggtc gatgcggttg tcctcggcgg
     7381 agtcgtgctc ggtctcgagg tgctgcgaac gcaggcgcag caactgaatg aagttgaagc
     7441 cgtcgatgat ggccgagact tcctccggac tcatgccgcc gatcttggtg ctgccctggc
     7501 gcaggcgctg caccgtgctg ctcgactcca cgccggtgcg cagggcgagg atgcgggcca
     7561 catcgacgaa caggcgggcg ccggatttct tcatgtcgat ggtgccgtct tcatccacca
     7621 cgaagtcgcg gatgcgcccg agcgggggca tgaccttgag cgcgttggcg gccatcatgc
     7681 gcaggaaggc gctgttgccc ttggcaccct tgttgagcgc gctgcgcagc tggtcgccga
     7741 agcgttcgtt gccgtagagc acgcggaagt cgaagaagat cgaggcgttg agcagggcct
     7801 gcgggtcggg ctcgcggatc cagtcgccga agcgcgactt ccactcgtcg agggtcaggc
     7861 agagctcggg gttgctcgcc atgatgttgc ctttgcacaa cgggaagccg caggccgcga
     7921 ggtcttcgtt gaccgtgcgc gcgaactcga ccaggcgggc gcgcgtgtcc ttgagttgct
     7981 tcggctcggc gcagcggaag atgatcccgt tgtcctggtc ggtggacagg gtctgctcgt
     8041 ggcggccctc ggagccgaag gcgagccagg cgaagtcgat ccccaccagc tcgtggcgtt
     8101 cacgcgccag gatgatgatg cggcgcgtca gcgcgtcgtt gagcgccgag atgaactgcg
     8161 tgagctgctc ggcgccgatg ccctgggcga tcaggttcag cgccagctga cggatgtcct
     8221 tgctcgcacg ctgcagggcc tcgatgtcgg cggcgttgtc gatgccggcg cggatctggc
     8281 gcaggctgat gcgctgcagc gagaacaggt cgcgctcgga gaccacgccc agcagcacgc
     8341 tgtcggagtc gaccacgagc aggtggcgca cgccgtgggt ggccatctcc agggccgcgt
     8401 cgtaggccga ggcgctcgcc ggcagctggt gcggattggc ggtcatcagc tcgccgatcg
     8461 ggcgcgcgag gtcgaagccg gcgaggatca cgcgcggcag caggtcgctc tgggtcagga
     8521 tgccgaccgg gcggcgttcc gcgtccacca ccaccatgca gccgagatgc agctcggaca
     8581 tgcgctcgag cgcgctgcgc gtcggcgtat cgggggtgac gaagaccgcc tccttctcga
     8641 tcaacttgcc gagcgccgtg gtcatggtct gctgctcggc ggcgcgctgg gcgaacgtgc
     8701 tctgcagctg ctggcgcgac tggctgagca ggctggcgat gtactgcgtg cagaagaggt
     8761 ggaacaccgg gctgatctgc atcagatgca ggaagtcgtc ggcgctgatc tggaagcaga
     8821 agctgtcctc gaccgccacg taggcgttgg tcgaggggcg gcgcgcggag atggcgccga
     8881 tcgggaagca ctcgcccggc ccgagggtga ggctggtgta ctcggtgacc gtggtctggc
     8941 cggtctgccg ggcctggatc ttgccgcgct ggacgatgta gaagtgcgac ggcacacccg
     9001 attcgggcgt caggatctcg ctgccacggg catggaaggc gagcaccgcg cgctcggcga
     9061 gaaaggcgag cgcgcccggc tccatgcggt tgaaggggga gaagttgcgc aggaaatcca
     9121 tgctggctcc caccagcagg tcggcggcgt cgttgctcat gggcggaccg ggatcgaagc
     9181 aatcgcacat tataggcgcg cgcatcgcgg gagcggcgag ccgcgacggc tgcgtcagag
     9241 cacctcggcg gcgtggtcgg cgaggcggga gcgttcgccg cgctgcaggg tgatgtggcc
     9301 ggagtgggcc cagcccttga aacggtcgac cacgaaggtg aggcccgaag aaccctcggt
     9361 gaggtagggc gtgtcgatct gcgcgatgtt gcccatgcag accaccttgg tgccggggcc
     9421 ggcgcgcgtg atcagggtct tcatctgctt gggcgtgagg ttctgcgcct cgtcgatgat
     9481 gaggaacttg ttgaggaagg tgcggccgcg catgaagttc aggctcttga tcttgatgcg
     9541 gctgcggatg aggtcgcggg tggccgcgcg gccccagtcg ccgccctcgc cggcggtgcc
     9601 gttgagcacg tcgaggttgt cctcgagcgc gcccatccag ggtgccatct tttcttcctc
     9661 ggtgccgggc aggaagccga tgtcctcgcc caccggaacg gtgacgcggg tcatgatgat
     9721 ctcgctgtag cgcttcgact ccagcacctg ggtgagcccg gcggcgagcg tcagcagggt
     9781 cttgccggtg ccggcctggc cgagcagggt gacgaagtcg atctcggggt tcatcagcag
     9841 gttgagcgcg aagttctgct cgcggttgcg cgcggtgatg ccccagacgt tgttcttgtg
     9901 gtggctgtag tcggtgaggg tctcgatgag ggcgctgcgg ccttcctttt ccttcaccca
     9961 ggcctgcagc gggttgtcgc cgtcctgata gacgaactcg ttcaccagca tctcggcggc
    10021 gagcggcccg ctgacgcggt agtagctgcg gccttcctcc ttccacgact gcatgccgcg
    10081 cgcgtggctg tcccagaagt cggcgggcag ctcgcaggtg ccggtgtaca gcaggtcgct
    10141 gtcctcgagc accttgtcgt tgaagtagtc ctgcgcggcg agcccgagcg cacgggcctt
    10201 gatgcgcatg ttgatgtcct tcgacaccag gatgacctcg cgcccggggt gcttctccac
    10261 caggaacatc accaccgcga ggatctggtt gtcgccgcgc gcggtcggca aggccggcgg
    10321 cagcttgatg tcgatcgcct cggtctgcag gtgcaggcgt ccggtggcct ggccgcgcga
    10381 ggggccctcg agcgcgatgc ccgcggcaat gccgtcttcc gaggcgcata cgatctcgtc
    10441 gagcatgcgg ctggcctggc gggcgttgcg tgccacctcg ctggtgccct tcttgttgtt
    10501 gtcgagttcc tccagcgtca tgatcggcac gtagaggtcg tgctcctcga agcggtacag
    10561 gctggtcggg tcgtgcatca gcacgttggt gtcgagcacg aagagcttgc acgctgccgg
    10621 ggccttgcgg gcacggcgga cacgcttgat ggcaggctcg cccgcgtccg cggggaggga
    10681 cggggaggtg gcggatttgc ggagggcgga catgacgtgg atccgggagg gcggcaggac
    10741 ggccgcagga agaggcgagg cggcgccggg gcgc
//
LOCUS       DAOQBS010000107        10603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_5515_length_10603_cov_14.874763, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000107 DAOQBS010000000
VERSION     DAOQBS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5515_length_10603_cov_14.874763"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..513
                     /locus_tag="PKN25_11620"
     CDS             277..513
                     /locus_tag="PKN25_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HNV91590.1"
                     /translation="MHTTLTSKGQVTIPKHIRDALHLEPGCAIEFRINREGEVVLTRP
                     SELRDEDDRFERLRGKADVKWRTDDLMALLRGDD"
     gene            513..911
                     /locus_tag="PKN25_11625"
     CDS             513..911
                     /locus_tag="PKN25_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV91591.1"
                     /translation="MLLVDTNALIDVLEDDPRWADWSIDMLRRQSQVHRLAINPIIYA
                     ELSLTFSTVEALDEAVTDFGVEVVEIPRPALFLAGKAFVAYRRNGGQRRNVLADFFIG
                     AHAAVEGWNILTRDPRKYRQYFPSVELVTP"
     gene            complement(986..1468)
                     /locus_tag="PKN25_11630"
     CDS             complement(986..1468)
                     /locus_tag="PKN25_11630"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNV91592.1"
                     /translation="MSSPLYDLEVDRLAGGTTTLGEYAGKLLLIVNTASQCGLTPHYA
                     GLEKLYQTYKDRGLVVLGFPCNQFGAQEPGSAEEIGAFCTRNYGVSFPMFAKIEVNGD
                     GAHPLYKYLKQHAKGILGTEAIKWNFTKFLVSRDSERIERYAPTTTPEELVKEIEGML
                     "
     gene            complement(1574..3946)
                     /locus_tag="PKN25_11635"
     CDS             complement(1574..3946)
                     /locus_tag="PKN25_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV91593.1"
                     /translation="MTRAAFALLLHTSSSIRLLVLALIGLAGLGWFVVEAGTSSAREH
                     LRYARDLREIRQVDAELGAAALARRLGLAEDWHGADAHLARMGELLEDLGRVPDFLTG
                     SEMAAVRRQLDHYTALQTDKRTVISALRVLLDAPPQNGGAAEAEADRLLRQLLALQTA
                     REGERLVESYTDGYVASQTRMHRHRVLLVLAALALGAYLLLTALRLRAVSLTVSKAHA
                     DLQEHAAALGRAEAELRLYATVFTNASEGMLITDAHSRIIAANPAFSRITGYSPAEVA
                     GRSPALLNSGRQDAGFYRRMWSTLASRGKWQGELWNRRRDGSIYPEWLSITAVPGEGE
                     GADGQPCNYIGIFSDITERKEAEARILHLAHHDPLTNLPNRALLNDRLSQSLLQARRD
                     GRGAAVLLLDLDRFKTINDTLGHERGDSLLLEICARCRRVLRDTDTLARLSGDEFVIV
                     LPDATTADVSADTARRILDAIALPCRLGEHEISITASIGIALFPRDGGDESTLLRNAD
                     AAMHRAKEAGRNGFEFYTEDMNTSSLGRLLLEHQLRGAAERGELLLHYQPKVSAADGR
                     LGGFEALLRWQHPELGMVAPGQFIPLAEESGLIVPIGQWVLEEACRQQRAWLDAGFDV
                     PPLAVNLSAHQLAQPDIVATVAAAIECHRLAPGLIELELTETMLMRDVDRTIRTLVRL
                     REMGVRLAIDDFGTGYSSLNYLRQFPVNALKIDRSFVSDIGSDGAEGKIAAAIVGMAH
                     SLGLDAVAEGVESEAQRAFLQMHGCHQLQGFLIARPAPAREIERFLSRRA"
     gene            complement(4146..4922)
                     /locus_tag="PKN25_11640"
     CDS             complement(4146..4922)
                     /locus_tag="PKN25_11640"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HNV91594.1"
                     /translation="MSSLATERVLSVHHWNESLFSFRTTRDPGLRFENGQFVMIGLDV
                     GGKPLTRAYSIASPNYEEHLEFFSIKVPDGPLTSRLQHLRPGDPIVVSKKPTGTLVLH
                     DLNPGKHLYLLATGTGLAPFLSVVQDPETYERFEKVVLVHGVRFVSELAYTDFITREL
                     PQHEYFGEQVREQLIYYPTVTREPFRNTGRITHVIDSGRLFADIGLPELDPGHDRVMI
                     CGSQAMNKDCCDLLDARGFKMSPRIGVAGDYVIERAFVEK"
     gene            5030..5956
                     /locus_tag="PKN25_11645"
     CDS             5030..5956
                     /locus_tag="PKN25_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV91595.1"
                     /translation="MKFTLRQIQVFVAVARAEHVSRAAEGLALSQSAASAALAELESQ
                     CAQPLFDRHGKRLRLNEQGSLLLPHAVELLDRAAEFEGRMRGEQGLGPLRVGATLTIG
                     NYLLPLIVSDFLQAHPESRVHLQVRNTATIAERLLHHEIDLGLVEGQVVDAELHVEPW
                     VEDELVVFCAPGHALAARGRASVAELAGQPWILRERGSGTRETFDRALRHHPGGVVPR
                     LELEHTEAIKRAVESGLGLGCLSRLALRDAFRRGSLVAVEVPELDLRRRFSFVWHRGK
                     YHGAALRRFVEDCRAFTAGARRSDEIPLPPVA"
     assembly_gap    6071..6170
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6201..7739)
                     /gene="murJ"
                     /locus_tag="PKN25_11650"
     CDS             complement(6201..7739)
                     /gene="murJ"
                     /locus_tag="PKN25_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNV91596.1"
                     /translation="MNLLRALATVSGMTLLSRILGFVRDLVIARAFGAGMATDAFFVA
                     FRLPNLLRRMFAEGAFSQAFVPILAEYKNRQGEAATHTLVSRVATLLGLVVAVVAALG
                     ALAAPLIIYVSAPGFSGDAGKFALTVELTRITFPYIFFMSLVALAGGVLNTWSRFAIP
                     AFTPVLLNLAFIGMALFAVPYFDPPVLALAWAVFLGGLLQLALQVRPLMKIGMMPRFD
                     LDLSDPGVRRVMTLMAPAILGVSVSQISLLINTIFASFLESGSVSWLYYADRLMEFPA
                     GLLGVALGTILLPSLSKLHADDKAEEFSSLLDWGLRLTLMLTLPAALGLALLAVPLVA
                     TLFNYGAFTPADVMQTRSALVAYSVGLTGLILVKVLAPGFYARQDIRTPVKIAILTLA
                     ATQLMNLAFVIPLRHAGLALSIGLASCLNAALLYRGLRKREVYIPQPGWGAFALKLLA
                     ALGVMGAALWFASGPASLWTGIGGLERGLRLALIVLGGAVAYFATLFALGFRLRDFRR
                     RGAA"
     gene            7910..8176
                     /gene="rpsT"
                     /locus_tag="PKN25_11655"
     CDS             7910..8176
                     /gene="rpsT"
                     /locus_tag="PKN25_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNV91597.1"
                     /translation="MANSAQARKRARQATKARAHNASLRSRLRTAIKAVRKAIVGGDK
                     AAAQSVFRTSMSTIDSIADKKIIHKNKAARHKSRLSAAVKAMAA"
     gene            complement(8356..8646)
                     /locus_tag="PKN25_11660"
     CDS             complement(8356..8646)
                     /locus_tag="PKN25_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HNV91598.1"
                     /translation="MNQKIENFIIVGVTREGKKFRPSDWSDRLCGVMSAFGADHRMTY
                     SPYVRPGCTLKGDKTVLVDARLYDVEPLAYKFMVNFANDNNLQIEWMGDAPF"
     gene            8971..10146
                     /locus_tag="PKN25_11665"
     CDS             8971..10146
                     /locus_tag="PKN25_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNV91599.1"
                     /translation="MSHVMNTYARLPVAFTHGEGVWLYDETGKRYLDALSGIAVNTLG
                     YKHPRLVKAIADQAERVLHTSNLYRIPLQEQLSDRIAEAAGMDEVFFCNSGCEANEAA
                     IKLARMYGHNKGIELPHIIVMENAFHGRTMATLSATGNRKAQAGFEPLVQGFIRVPYK
                     DIEAIRRIAEHNHTVVAVMLEMIQGEGGVNVADEAFQRDLRALCDDRGWLMICDEVQC
                     GMGRTGKWFGWQHAGTRPDVMTLAKGLASGVPIGACVTSGLAKGLFGPGNHGSTFGGN
                     PLACAAGLATFDAIVEDELMDNAVAVGAAIRKGMAEALAGEAGVVDIRGRGLMIGIEL
                     DRPCGVLMARAAENGLLLSVTSERVVRLLPALTFTTADAQTLVSMLAPMIRDFLANG"
     gene            10156..>10603
                     /locus_tag="PKN25_11670"
     CDS             10156..>10603
                     /locus_tag="PKN25_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNV91600.1"
                     /translation="MSSPIKHYLQFKDFTREEYEYVFARATWIKDKFKRYEPYHPLFD
                     RTLVMIFEKASTRTRLSFEAGMHQLGGSAIYLNTRDSQLGRGEPVEDAGQVISRMSDL
                     VMIRTFEQDIVERFAANSRVPIINGLTNEYHPCQILADILTFIEHRG"
BASE COUNT         1677 a         3394 c         3733 g         1699 t
ORIGIN      
        1 ccgttgaggc cgagcaggcc gatcttggcg ccggggaaga aggacagcga gatgtccttg
       61 atgatctgac gcttgggcgg aacgatcttg ctcacgcgca gcatcgacat gacgtattgg
      121 gccactgcgg gtacctcgtt ggcggtgttc ggtggggcgg aaaaggcgga agtctacccg
      181 agtgggcagc ggcaggcacg catggagctt gcagctagcc gattggggta ttacgctgcg
      241 aagacgtgta agaaatacag gaaggagaga atcgagatgc acaccacact gacgagcaag
      301 gggcaggtga cgatccccaa gcacatccgc gacgcgctgc atctcgagcc cggatgcgcg
      361 atcgagttcc gcatcaatcg cgagggtgag gtcgtcctga cgcgccccag tgaactacga
      421 gacgaggatg atcgcttcga gcgcttgcgc ggcaaggccg acgtcaagtg gcggacggat
      481 gacctgatgg ccttgctgcg tggcgacgac tgatgctgct ggtcgacacc aatgcgctga
      541 tcgatgtgct ggaagacgat ccgcgctggg cggattggtc catcgacatg ctgcgtcggc
      601 agtcgcaagt gcatcgactt gcgatcaatc ccatcatcta cgccgaactt tccttgacct
      661 tctcgaccgt cgaggcgctg gatgaggcgg tcaccgattt cggcgttgaa gtcgtcgaga
      721 tccccaggcc ggcgctcttt ctcgcgggca aggccttcgt cgcctatcgt cgaaacggcg
      781 gccagcggcg caacgtgctg gccgacttct tcatcggcgc gcatgccgcg gtcgaaggct
      841 ggaacatcct tacgcgagat ccgcgcaagt accgacagta ctttccctcg gtcgagcttg
      901 tgacgccctg aatcctgtcg cgggaccacg tccgcccccc gccttggggc gcacgaatcc
      961 ggcccagcgc atgcgtctcc cttgcctaga gcatcccctc gatctccttc accagttcct
     1021 ccggcgtggt ggtcggcgcg tagcgctcga tgcgctcgct gtcgcggctc accaggaact
     1081 tggtgaagtt ccacttgatc gcctcggtgc cgaggatgcc tttcgcgtgc tgtttcagat
     1141 acttgtacag cggatgtgcg ccgtcgccgt tgacctcgat cttggcgaac atcggaaagc
     1201 tcacgccgta gttcctggtg cagaaggcgc cgatctcctc cgcgctaccc ggctcctgcg
     1261 ctccgaactg gttgcacgga aagccgagca ccaccaggcc gcggtccttg taggtctgat
     1321 agagcttttc caggccggcg taatgcgggg tgagcccgca ctggcttgcg gtgttgacga
     1381 tcagcagcag cttgccggcg tattcgccga gcgtggtggt gccgccggcg aggcggtcga
     1441 cttcgaggtc gtacaggggc gagctcatgg gggagggtct ccgtgcgcgg gtgggatgcg
     1501 ggcccgccgc atggcggact tcgcaggctt ggaggcgggc gggccggcgc aagtttccgc
     1561 cgagccgcgc gattcaggcg cgccggctca ggaagcgctc gatttcgcgc gcaggcgccg
     1621 ggcgggcgat caggaagccc tgcaactgat ggcagccgtg catctgcagg aaggcgcgtt
     1681 gggcctcgct ctccacgcct tcggcgaccg cgtccagccc caggctgtgg gccatgccga
     1741 cgatggctgc ggcgatcttg ccctcggcgc cgtcgctgcc gatgtcgctg acgaagctgc
     1801 ggtcgatctt cagcgcattg accgggaact ggcgcaggta gttgagcgag gagtagccgg
     1861 tgccgaagtc gtcgatcgcc aggcgcacgc ccatctcgcg caggcgcacg agggtgcgga
     1921 tggtgcgatc gacgtcgcgc atcagcatgg tctcggtgag ctcgagctcg atcaggccgg
     1981 gcgcgaggcg gtggcactcg atggccgccg ccacggttgc gacgatgtcg ggttgggcga
     2041 gctggtgcgc cgagaggttc accgcgagcg gcggcacgtc gaaaccggcg tccagccagg
     2101 cgcgctgttg gcggcaggct tcctcgagca cccattggcc gatcggcacg atcaggcccg
     2161 attcttcggc gagcgggatg aactggccgg gcgcgaccat gccgagctcg ggatgctgcc
     2221 agcgcagcag cgcctcgaag ccgccgaggc ggccgtcggc ggcgctcacc ttgggctggt
     2281 agtgcagcag cagctcgccg cgctcggcgg cgccgcgcag ctggtgttcg agcaacaggc
     2341 ggccgagcga actggtgttc atgtcctcgg tgtagaactc gaaaccgttg cgcccggcct
     2401 ccttggcgcg atgcatggcg gcgtcggcat tgcgcaacag ggtcgattcg tcgccgccgt
     2461 cgcgcgggaa gagggcgatg ccgatgctcg ccgtgatgct gatctcgtgc tcgcccagcc
     2521 ggcagggcag ggcgatggcg tcgaggatcc tgcgcgcggt gtctgcgctg acgtcggcgg
     2581 tggtggcgtc gggcagcacg atgacgaact cgtccccgct caggcgggca agcgtgtcgg
     2641 tgtcgcgcag cacgcgccgg cagcgtgcgc agatctccag cagcagactg tccccgcgtt
     2701 cgtggccgag ggtgtcgttg atcgtcttga agcggtcgag gtcgagcagc agcaccgcgg
     2761 cgccgcggcc gtcgcgccgt gcctgcagca ggctctggct caggcggtcg ttgagcagcg
     2821 cgcggttggg caggttggtg agcgggtcgt ggtgggcgag gtgcaggatg cgcgcttcgg
     2881 cctccttgcg ttcggtgatg tcggagaaga tgccgatgta gttgcacggc tgtccgtccg
     2941 ccccctcgcc ctcgcccggc accgcggtga tcgacagcca ctcgggatag atcgagccgt
     3001 cgcggcggcg gttccacagt tcgccctgcc acttgccgcg gctggcgagc gtggaccaca
     3061 tgcgccggta gaagccggcg tcctgccgcc cggaattgag cagcgccggc gagcgccccg
     3121 cgacctccgc cgggctgtag ccggtgatgc gggagaaggc cgggttggcg gcgatgatgc
     3181 ggctgtgcgc atcggtgatc agcatgccct cgctggcgtt ggtgaatacc gtggcgtaca
     3241 ggcgcagctc ggcttcggca cggccgagcg cagcggcgtg ttcctgcagg tcggcatgcg
     3301 ccttgctgac agtgagcgag accgcgcgca gtcgcagcgc ggtgagcagc aggtaggcgc
     3361 cgagcgcgag cgcggcgagc accagcagca cgcggtggcg gtgcatgcgg gtttgcgagg
     3421 cgacgtagcc gtcggtgtag ctctccacca ggcgctcgcc ctcgcgtgcg gtctgcagcg
     3481 cgaggagctg gcgcaggagg cggtcggcct cggcttcggc cgcgccgccg ttctgcggcg
     3541 gcgcgtcgag gagcacgcgc agcgcgctga tcacggtgcg cttgtcggtc tgcagggcgg
     3601 tgtagtgatc gagctggcgg cgcacggccg ccatctcgct gccggtgagg aagtcgggaa
     3661 cgcggccgag gtcttcgagg agttcaccca tccgcgccag gtgggcgtcg gccccgtgcc
     3721 agtcctcggc gagcccgagc cggcgggcga gcgcggcggc gccgagctcg gcatcgacct
     3781 ggcggatctc gcgcaggtcg cgggcgtagc gcaggtgctc gcgtgccgag ctggtgccgg
     3841 cctcgaccac gaaccacccc agcccggcga ggccgatcag cgcgagcacg agcaggcgga
     3901 tgctgctcga ggtgtgcagc agcagcgcga aggcggcgcg ggtcacttcc tccgctccgg
     3961 agcgaggggc cggggagcgg ggggcaaggt tgagccgggg agaagggagg tgggcaaggc
     4021 gggcacgggc ggatcctatc tgcgcgtctt gccctggaat tgatctatat caaaggttgg
     4081 atgataggac atccgggtcg ggcatgccga tccactatgc cgggatggct ccttccacga
     4141 cagcttcact tctccacgaa cgcgcgctcg atcacgtagt cgcccgccac gccgatgcgc
     4201 ggcgacatct tgaagccgcg cgcgtcgagc aggtcgcagc agtccttgtt catcgcctgg
     4261 ctgccgcaga tcattacgcg gtcgtgcccg ggatcgagct ccggcaggcc aatgtcggcg
     4321 aagagccgac cggagtcgat cacatgagtg atgcgtccgg tgttgcggaa gggctcgcgc
     4381 gtcaccgtcg gatagtagat cagctgctcg cgcacctgct cgccgaagta ttcatgctgc
     4441 ggcagctcgc gggtgatgaa gtcggtgtaa gcgagttcgg agacgaaacg cacaccatgc
     4501 accagcacga ccttctcgaa gcgctcgtag gtctccgggt cctggaccac gctcaggaag
     4561 ggcgccaggc cggtgccggt ggcgagcagg tagaggtgtt tgcccgggtt gaggtcgtgc
     4621 agcaccaggg tgccggtggg cttcttgctc accacgatcg ggtcgcccgg gcgcaggtgt
     4681 tgcaggcgcg aggtcagcgg gccgtccggc accttgatgc tgaagaactc gaggtgctcc
     4741 tcgtagttgg ggctggcgat gctgtaggcg cgcgtcagcg gcttgccgcc gacgtccagg
     4801 ccgatcatca cgaactggcc gttctcgaag cgcagcccgg ggtcgcgggt ggtgcggaag
     4861 ctgaagaggg attcgttcca gtgatggacg ctgaggacgc gttcggtggc gaggctgctc
     4921 atggtttcgg gtctctgcag gctgcggaat gaaggagtga gcggattcta ggcacttctc
     4981 cttacctgta tggtggataa tattcatatc cttcatcgac tttatcgata tgaagttcac
     5041 cctccgccag atccaggtct tcgtcgcggt ggcgcgcgcc gagcacgtct cgcgcgctgc
     5101 cgagggcctc gcgttgtcgc agtcggcggc gagtgcggcg ctcgccgagc tggagagtca
     5161 gtgcgcgcag ccgctcttcg accgccacgg caagcgcctg cgcctgaacg agcagggcag
     5221 tctgctgctg ccgcacgcgg tggagctgct cgaccgtgcc gccgagttcg agggccgcat
     5281 gcgcggcgag cagggcctcg gcccgctgcg cgtgggcgcg acgctgacca tcggcaacta
     5341 cctgctgccg ctgatcgtgt cggatttcct ccaggcccac cccgagagcc gggtgcatct
     5401 gcaggtgcgc aacaccgcga ccatcgccga gcgcctgctg caccacgaga tcgacctggg
     5461 tctggtcgag ggccaggtgg tggatgccga gctgcacgtc gagccctggg tcgaggacga
     5521 gctcgtggtg ttctgcgcgc cggggcatgc gctcgccgca cgcggtcgcg cgagcgtggc
     5581 cgagctcgcc ggccagccgt ggatcctgcg cgagcgtggt tcgggcacgc gcgagacctt
     5641 cgatcgcgcg ttgcggcatc acccgggcgg ggtcgtgccg cggctggagc tcgagcatac
     5701 cgaggccatc aagcgcgcgg tggagagcgg cctgggcttg ggttgcctgt cgcggctggc
     5761 gctgcgcgat gcgttccggc gcggcagcct ggtggcggtg gaggtgcccg agctcgacct
     5821 gcgccggcgc ttcagcttcg tctggcatcg cggaaaatat cacggtgccg cgctgcgccg
     5881 cttcgtcgag gattgccgcg ccttcaccgc cggcgcccgc cgcagcgacg agatcccgct
     5941 gccgccggtg gcttgaattc gtcgcggtgt ttcgttcggc gctgcgatcg cgacttgcgt
     6001 cgctcccaca atgtgcgacg ctgtcatcgc gacttgcgtc gctcccacaa tgtgcgacgc
     6061 tgtcatcgcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aggagcgacg
     6181 ccagtcgcga tatcagcgcc tcaggccgcg ccgcggcggc ggaagtcgcg caggcggaag
     6241 ccgagtgcga acagggtggc gaagtaggcc accgcgccgc cgagcacgat cagggccagg
     6301 cgcaggccgc gctcgagacc gccgatcccg gtccacaggc ttgccggccc gctcgcgaac
     6361 cagagcgcgg cacccatgac gcccagtgcc gccagcagct tgagcgcgaa cgccccccag
     6421 cccggctgcg gaatgtacac ctcgcgcttg cgcagaccac ggtagagcag ggcggcgttg
     6481 aggcaggacg ccaggccgat cgacagcgcc aggccggcgt ggcgcagcgg gattacgaag
     6541 gcgaggttca tcagctgcgt cgcggccagg gtcaggatgg cgatcttcac cggcgtgcgg
     6601 atgtcctggc gggcgtagaa gcccggtgcg agcaccttca ccaggatcag cccggtcagg
     6661 cccacgctgt aggcgaccag cgcgctgcgc gtctgcatca cgtcggcggg ggtgaaggcg
     6721 ccgtagttga acagcgtcgc caccagcggc accgccagca gcgccaggcc gagcgcggcg
     6781 ggcagggtca gcatcagggt caggcgcagg ccccagtcga gcagcgagga gaactcctcc
     6841 gccttgtcgt cggcgtgcag cttggagaga ctggggagca ggatggtgcc gagcgccacg
     6901 ccgagcaggc cggcgggaaa ttccatcagg cggtccgcgt aatacagcca ggacacgctg
     6961 ccgctctcca ggaaggaggc gaagatggta ttgatcagca gcgagatctg gctcaccgac
     7021 acgcccagga tcgccggcgc catcagcgtc atgacgcggc gcacgccggg gtcggagagg
     7081 tcgaggtcga agcgcggcat catgccgatc ttcatcagcg gccgcacctg cagcgcgagc
     7141 tgcagcaggc cgccgaggaa caccgcccac gccagcgcca gcaccggggg gtcgaagtac
     7201 ggcaccgcga acagcgccat gccgatgaag gcgaggttga gcagcaccgg cgtgaaagcc
     7261 gggatcgcga accggctcca ggtgttgagc acgccgcccg cgagcgccac cagcgacatg
     7321 aagaagatgt aggggaaggt gatgcgggtg agctcgacgg tgagcgcgaa cttgcccgcg
     7381 tcgccggaga agcccggggc cgacacgtag atgatcagcg gtgcggcgag cgcgccgagc
     7441 gccgcgacca ccgccaccac cagcccgagc agggtggcga cccggctcac cagagtgtgg
     7501 gtggcggcct cgccctggcg gttcttgtac tcggcgagga tgggtacgaa ggcctgcgag
     7561 aacgcgccct cggcgaacat gcggcgcagc aggttgggca ggcggaaggc gacgaagaag
     7621 gcgtcggtcg ccatgccggc gccgaaggcg cgcgcgatca cgaggtcgcg gacgaagccc
     7681 aggatgcggg acagcagggt catgccgctg acggtggcga gcgcgcgcag gaggttcatt
     7741 cggtcgaggc gcgcgccggc cggcggcgcg cggaagatgc ggagggcgca tcatagccgc
     7801 ccgccggtgt ctcgataagc gggagtgcgg gaaaagacgc ttgcattcac cggagccggt
     7861 ccctataatc gcggactttt ccagaaccac caatacggac ttcaaatcta tggccaactc
     7921 ggcacaagct cgcaagcgcg cccgtcaggc gaccaaggcc cgcgcccaca acgccagcct
     7981 tcgttcccgc ctgcgtaccg cgatcaaggc cgtgcgcaag gccatcgtgg gtggcgacaa
     8041 ggcggcggct cagtcggtct ttcgcacctc gatgagcacc atcgacagca tcgccgacaa
     8101 gaagatcatc cacaagaaca aggctgctcg ccacaagagc cgcctgtcgg ccgccgtcaa
     8161 ggcgatggct gcctgatcct tgccgcatgc cggcgcctcg gcgccgagat cgagaaagcg
     8221 acccgagggt cgcttttttg ttgggcgtat gccaggaagg cctcggcgcg gactccctcg
     8281 gcgcggactc ccggtcccgg ggtcgggact tcgtcgctct tgcggttccg gagcggaggg
     8341 gacgagtgcg ccgtctcaga acggcgcgtc gcccatccac tcgatctgga ggttgttgtc
     8401 gttggcgaag ttcaccatga acttgtaggc cagcggttcg acgtcgtaga ggcgggcgtc
     8461 gacgaggacg gtcttgtcgc ccttcagcgt gcaccctggg cgcacgtagg gcgaatacgt
     8521 catccgatgg tcggcgccga aggcggacat cacgccgcac aggcgatcgg accagtcgct
     8581 cggacggaat ttcttcccct ccctcgtgac cccgacgatg atgaagttct cgatcttctg
     8641 attcatgtgt gatgccttgg cggcttcggg gatgcgcatg ttccggttct ttgtcgccgg
     8701 aatcaggacg ggcggcattc tagcagaaat cccccgcggc cattccgccg cgcgggcgtc
     8761 gggcgatcct gccgaagggc ttcgtgcgac ctgtcggcgc cggggctccg gtcgtgctga
     8821 cgaggctcgc ggatggcgtc cggcgtcccg cggagcgcgg cccgcttatg tacagggtcg
     8881 gccgttttgg cttaacatcg gcacatttcc tgcacggcgg cgtctgccgc cgtgtgcgtt
     8941 ttccggcttc gcaccgatcg aggtaccccc atgtcccatg tcatgaatac ctacgcccgc
     9001 ctgcccgtgg ccttcacgca cggcgagggc gtgtggctgt acgacgagac aggcaagcga
     9061 tatctcgatg ccctctccgg catcgcggtc aacacgctcg ggtacaagca cccccgcctg
     9121 gtcaaggcca tcgccgacca ggccgagcgc gtcctgcaca cctccaacct gtatcgcatc
     9181 ccgctgcagg agcagctctc cgaccgcatc gccgaagccg cgggcatgga cgaggtgttc
     9241 ttctgcaact ccggctgcga ggccaacgag gcggcgatca agctcgcgcg catgtacggc
     9301 cacaacaagg gcatcgagct gccgcacatc atcgtcatgg agaacgcctt ccacggccgc
     9361 accatggcca cgttgtcggc caccggcaac cgcaaggccc aggccggttt cgagccgctg
     9421 gtgcagggct tcatccgcgt gccctacaag gacatcgagg ccatccgcag gatcgccgag
     9481 cacaaccaca ccgtggtcgc ggtgatgctc gagatgatcc agggcgaggg cggggtcaac
     9541 gtcgccgacg aggccttcca gcgtgatctg cgcgccctgt gcgacgacag gggctggctg
     9601 atgatctgcg acgaggtcca gtgcggcatg ggccgcaccg gcaagtggtt cggctggcag
     9661 catgcgggca cgaggcccga cgtgatgacg ctggccaagg gcctggcctc gggcgtgccg
     9721 atcggcgcct gcgtgaccag cggcctggcc aagggcctgt tcgggccggg caaccatggc
     9781 tccaccttcg gcggcaaccc gctcgcctgc gcggcgggcc tggccacctt cgacgccatc
     9841 gtcgaggacg agctcatgga caacgccgtc gccgtcggcg cggcgatccg caagggcatg
     9901 gccgaggcgc tcgccggcga ggcgggcgtg gtcgacatcc gcggtcgcgg cctcatgatc
     9961 ggcatcgaac tcgaccgccc ctgcggcgtg ctgatggcgc gcgcggccga gaacggcctg
    10021 ctgctgtcgg tgacctccga gcgcgtggtg cgcttgctgc cggcgctcac cttcaccacc
    10081 gccgacgcgc agaccctggt gtccatgctg gcgccgatga ttcgcgactt cctcgcgaac
    10141 ggctgaggac tgcgtatgag ctctccaatc aagcactacc tccagttcaa ggatttcacc
    10201 cgcgaggaat acgaatacgt gttcgcgcgc gcgacgtgga tcaaggacaa gttcaagcgc
    10261 tacgagccct accacccgct cttcgaccgc acgctggtga tgatcttcga gaaggcgagc
    10321 acgcgcacgc gcctctcgtt cgaggctggc atgcaccagc tcggcggctc ggcgatctac
    10381 ctcaacaccc gcgactcgca gctcggccgc ggcgagccgg tggaggacgc cggccaggtg
    10441 atctcgcgca tgagcgacct ggtgatgatc cgtaccttcg agcaggacat cgtcgagcgc
    10501 ttcgccgcca actcgcgcgt gccgatcatc aacggcctca ccaacgagta ccacccctgc
    10561 cagatcctcg ccgacatcct caccttcatc gagcaccgcg gct
//
LOCUS       DAOQBS010000108        10546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_5560_length_10546_cov_19.068249, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000108 DAOQBS010000000
VERSION     DAOQBS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10546
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5560_length_10546_cov_19.068249"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PKN25_11675"
     CDS             <1..630
                     /locus_tag="PKN25_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV91601.1"
                     /translation="LEQGSKYEVAVILESAEPGLIKGRARTFFEFTPLTDDAGDRTIY
                     LPSVDSAGNFNFDIEVRAGETILIDPVVAVGYDYQIGSGDPLFASVTLPNVGDGIFEL
                     WLYDLNLADFVFETFLDAGERFDFTGGGVDRFRVLGIEPSAGLDPTDVQAFVTEIGFT
                     GNGRFTGRMTPITTNFTPVPEPSSLALLALAVLALGGLRRSCRAGGSLR"
     gene            665..943
                     /locus_tag="PKN25_11680"
     CDS             665..943
                     /locus_tag="PKN25_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNV91602.1"
                     /translation="MSNKHRHTLEAIFRDPPAGNLHWREVESLLSHLGAEVESGHGAR
                     FRVVLNRREFYLHHPHHGSDFGKQAVKDLREFLASAGVSPSSYQDGTG"
     gene            complement(1068..2456)
                     /locus_tag="PKN25_11685"
     CDS             complement(1068..2456)
                     /locus_tag="PKN25_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HNV91603.1"
                     /translation="MNDILRVAVVGHTNTGKTSLLRTLARDVGFGEVSDSAGTTRHVE
                     GLRLMADGVPAAELFDTPGMEDAIALLEFIDDLVDPGERVDGPERVARFLARDEAAGR
                     FEQEAKVLRQMLASDAALYVVDARDPVLPKHRDELELLAACARPLLPVLNFIADPVAR
                     ADDWRGALARLGLHAVVSFDTVAPPLDGERELFDTLATLIHSHRGRLQALIEARAREA
                     AARREAARRLVAELLVELAARRDRLADSEDDEAALQRAVERLRADVRSREQACVDALL
                     RLYRFRADDARMAELPLADGRWDDDLFNPETLRQMGVRLGTGAAAGAAAGVGIDLMTG
                     GLTLGAAAALGAIAGGLWQTFGHYGERLAAKLRGHRELTVDDAILRIVALRQRSLLVA
                     LDGRGHAAVKPIELDAGRAGMPMIADPVSVRWRKDALPEAIGRARAHPEWAEADGGHK
                     DEAIAELARALA"
     gene            complement(2453..3907)
                     /locus_tag="PKN25_11690"
     CDS             complement(2453..3907)
                     /locus_tag="PKN25_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HNV91604.1"
                     /translation="MPTSFPPNSLSLRWLAELVRRHEERHPPLDEARAHAEARAVAGD
                     LEARVLRRAAVLEAAGGWREAIRRWQGRARLLLVLACVLAFVFGFGAAAGALGDGTRP
                     VNVVWTLGGLLGVHLLSLLLWLASLMLQGRAQGGALQHGGLLGRAWLAVTGWLDRSRE
                     AADLPLALGGLLGSGRSAAWGVGAANHALWFGALSGATLGVLALLATRRYGFVWETTI
                     LPADTFVALSAALGALPGMLGFPVPDAATVLASGDAPMLDEAGRRAWAGWLVGAMTIY
                     GVLPRLALALLCFGLWRRATRALALDLGRPGYARLRPLLMPDSERIGVRDPEPAAMPH
                     PQRHAAVAHGDGGAVAVALELGDDLAWPHALAGAHDAAVALAWQDGGRLDSREQRRSV
                     RARFAAHPPVRLVVAVDARQTPDRGSLGLIAELADHAQATRIWLAGVDAAAEQAGRLR
                     QWREGLAGIGLGEAAALADAGAACGWLERGDEAR"
     gene            4116..4592
                     /locus_tag="PKN25_11695"
     CDS             4116..4592
                     /locus_tag="PKN25_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNV91605.1"
                     /translation="MSTDLFHWTDEFSIGLQEIDEQHKELVELLNQLHVAIKEHHGST
                     TSRQILDKLADYTRTHFAVEESLMRVSNYPEFATHKQNHEDLIAQVQALQDKLDSGQA
                     TITFELLHFLKVWLTRHINEADKRFGAYFVEAGGASKWSDHVEQAMKKRKWWWKFW"
     gene            4658..5143
                     /locus_tag="PKN25_11700"
     CDS             4658..5143
                     /locus_tag="PKN25_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91606.1"
                     /translation="MQHTSTVRLALLALVLAGSLGATAAHAEALACPTLSTATQVQPC
                     PTEDELKYTYTGYCSDNARLYGRDVLTCASFENYKEVKNVALWEAEGGKFDGYLSCNV
                     DAASIKASKAQKLSVERVKTLTRVICDYENDHRLVHRTKSECVVEAADCSGAECKARC
                     D"
     gene            complement(5223..6356)
                     /locus_tag="PKN25_11705"
     CDS             complement(5223..6356)
                     /locus_tag="PKN25_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNV91607.1"
                     /translation="MNIPAQSADVDRRIPVTLLTGFLGAGKTTLLNHLLRQPQMEGSA
                     VLINEFGAVGVDHHLVEKVDESLVVLDSGCICCSVQGDLVRALKSLFMRALRRELKGL
                     RRVLIETTGLADPAPVIHTLMAEPFLSERYRLDGVVTAVDLTHAREQLAHHNEAVRQV
                     AMADRVLLTKGDLAADAMRAEVGALVAALNPGARQIEVAGGEVGPEAVFGCGLYDPAG
                     KTPDVAAWLGEEAVRAARRAPVAPLWRRPGRSGAGVHSLTAAEQENSRHTPGVSAFVL
                     VFDEPLDWFGFSDGLALLLQVYGGRILRIKGLLNVAGDPLPRVLQCVQHSVYPASSLP
                     AWPDSAPYDDRRSRLVFIVRELAEDEVISILGSFTGQLPRRTD"
     gene            complement(6594..8072)
                     /gene="adeC"
                     /locus_tag="PKN25_11710"
     CDS             complement(6594..8072)
                     /gene="adeC"
                     /locus_tag="PKN25_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AdeC/AdeK/OprM family multidrug efflux complex
                     outer membrane factor"
                     /protein_id="HNV91608.1"
                     /translation="MKTHAKALLVPDSVPVSSVRRGRAATPLRSLVVALAAATLGACS
                     MIPVFERPAAPVPATFPQATPTAAPVVAPLADTIAWRDYFADQRLREVIALALENNRD
                     LRVAALNIERARAQYGIQQADLFPSIAVSGGQSAQRVPGDLSMTGDSNVSRQYSANLG
                     FASYELDFFGRVRSLEEQALQNYLATEEARRSAQISLVAEVANAWLRLAADRERLALA
                     HNTRQTRQASFDLVRRSLELGAVSALDVHQAETLLQSARADAARFETVVAQDRNVLAQ
                     LVGGGVPDTLLPDGLEQAVATVAELPAGVPSEVLTRRPDIVQAERALLAANASIGAAR
                     AAFFPRITLTASAGTASSTLDGLFDGGSGAWSFVPQLRLPIFEAGRLQASLDLAEIQR
                     DINVAQYEKAIQSAFREVADALAERATVAERIDARRKQVAATQNSFKLSDARYRGGVD
                     SYLSLLDAQRSLYAAELELIGVRLIEATNRVELYKALGGGWQ"
     gene            complement(8074..10546)
                     /locus_tag="PKN25_11715"
                     /pseudo
     CDS             complement(8074..10546)
                     /locus_tag="PKN25_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584477.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT         1678 a         3724 c         3709 g         1435 t
ORIGIN      
        1 ctggagcagg ggagcaagta cgaagtggcg gtgatcctcg aatccgccga acccggcctg
       61 atcaagggcc gcgcacgcac cttcttcgag ttcaccccgc tcacggacga cgccggcgac
      121 aggacgatct acctgccgag cgtggacagt gcgggaaact tcaacttcga catcgaggtg
      181 agggccggcg agaccatcct gatcgatccg gtggtcgcgg tcggctatga ctatcagatc
      241 ggcagcgggg atccactgtt cgcaagcgtc accctgccga acgtcggtga cggaatcttc
      301 gaactctggc tttacgacct gaacctcgcc gacttcgtgt tcgaaacctt tctggacgca
      361 ggagagcgct tcgacttcac gggcggcggc gtggatcggt tccgcgtgct cggcatcgag
      421 ccgtcggcag gactggatcc gaccgatgtg caggctttcg tgaccgagat cggtttcacc
      481 gggaatggcc ggtttaccgg tcgcatgacc ccgatcacca ccaatttcac tcccgtgccg
      541 gaaccgtcct cgctcgcgct gctggccctg gcggtgctgg cgctcggagg actgcgacgc
      601 agttgccgcg ccggaggctc gctgcgctag catgagcgga ttcccgcttc gcccggagtc
      661 cgccatgagc aacaagcatc gccacaccct ggaagcgatc ttccgcgatc cgccggccgg
      721 caacctgcac tggcgcgagg tcgagtcgct gctgagccac ctcggcgccg aggtcgaaag
      781 cggccacggc gcgcgcttcc gcgtcgtgct gaaccggcgc gagttctacc tgcaccaccc
      841 gcaccacggc agcgacttcg gcaagcaggc ggtgaaggac ctgcgcgaat tcctcgccag
      901 cgccggcgtg tcgccgtcga gctaccagga cggcacgggc tgacgcagcg cgcccgaccg
      961 catcgcggtc gcggggcttc gccgccgcgc cacgcgcccg gaggcgtgcg acgcaggaat
     1021 ccgggcgctc cagccggcag gccgcacgcg tcctcgacgg gcgtcgctca ggcgagcgcg
     1081 cgcgcgagct ccgcgatcgc ctcgtccttg tggccgccgt ccgcctccgc ccactcgggg
     1141 tgggcgcggg cgcggccgat cgcctcgggc aatgcatcct tgcgccagcg cacgctcacc
     1201 gggtcggcga tcatcggcat gcctgcgcgc ccggcatcga gctcgatggg cttcaccgcg
     1261 gcgtggccgc gcccgtccag cgcgaccagc aggctgcgct ggcgcagcgc gacgatgcgc
     1321 aggatggcgt cgtccacggt gagctcgcgg tggccgcgca gcttggcggc gaggcgctcg
     1381 ccgtagtggc cgaaggtctg ccacaggccg ccagcgatgg cgccgagggc ggcggcggcg
     1441 cccagcgtga ggccgccggt catcaggtcg atgccgacgc cggctgccgc gcccgcggcg
     1501 gccccggtgc cgaggcgcac gcccatctgg cgcagggtct cggggttgaa gaggtcgtcg
     1561 tcccagcgtc cgtcggcgag cggcaactcg gccatgcgcg cgtcgtcggc gcggaagcgg
     1621 tacaggcgca gcagcgcgtc cacgcaggcc tgctcgcgac tgcgcacgtc cgcgcgcagg
     1681 cgctcgaccg cgcgctgcag cgcagcctcg tcgtcctcgg aatcggcgag gcggtcacgg
     1741 cgcgcggcga gctcgaccag cagctcggcg accaggcggc gggcggcttc gcgacgcgcc
     1801 gcggcctcgc gcgcacgcgc ctcgatcagg gcctgcaggc ggccgcggtg gctgtggatc
     1861 agcgtcgcca gggtgtcgaa gagttcgcgc tcgccgtcga gcggcggcgc cacggtgtcg
     1921 aagctgacca ccgcgtgcag accgaggcgg gcgagcgcgc cgcgccagtc gtccgcccgc
     1981 gccaccggat cggcgatgaa gttgagtacc ggcagcagcg gtcgcgcgca cgcggcgagc
     2041 agctcgagtt cgtcacggtg cttgggcagc accggatcgc gcgcatccac cacatagagc
     2101 gcagcgtcgc tcgccagcat ctggcgcagc accttggcct cctgctcgaa gcggccggcg
     2161 gcctcgtcgc gggcgaggaa gcgcgccacg cgctcggggc cgtcgacacg ctcgccgggg
     2221 tcgaccagat cgtcgatgaa ctcgagcagc gcgatcgcgt cctccatgcc cggggtgtcg
     2281 aagagctccg ccgccggcac gccgtcggcc atcaggcgca ggccctcgac gtggcgggtg
     2341 gtgccggcgg aatcggagac ctcgccgaag cccacgtcgc gcgcgagcgt gcgcaacagc
     2401 gaggtcttgc cggtgttggt gtggccgacc acggccacgc gcaggatgtc gttcatcggg
     2461 cctcgtctcc ccgttccagc cagccgcacg cggcgcccgc gtccgccagc gccgccgcct
     2521 cgcccaggcc gatcccggcc aggccctcgc gccactggcg cagccgcccg gcctgctccg
     2581 ccgcggcatc gacgccggcc agccagatgc gggtcgcctg cgcgtgatcg gcaagttcgg
     2641 cgatgaggcc gaggctgccg cgatccgggg tctggcgggc atcgacggcg accaccaggc
     2701 gcaccggcgg gtgggcggcg aagcgcgcgc gcacgctgcg gcgctgctcg cggctgtcga
     2761 gtcggccgcc gtcctgccag gcgagcgcga ccgcagcgtc gtgtgcgcca gcgagcgcat
     2821 gcggccacgc caggtcgtcg ccgagttcga gcgccacggc caccgctccg ccgtcgccat
     2881 gggcgaccgc ggcgtggcgc tgcgggtggg gcatcgccgc cggctcgggg tcgcgcacgc
     2941 cgatgcgttc gctgtcgggc atcagcagcg ggcgcaggcg ggcgtagcct gggcggccga
     3001 ggtcgagcgc aagcgcacgc gtcgcgcgcc gccacagccc gaagcagagc agcgcgagcg
     3061 ccagccgcgg cagcacgccg tagatcgtca tcgcccccac cagccagccg gcccaggcgc
     3121 gccggccggc ctcgtccagc atcggggcgt cgccgctcgc cagtaccgtg gcggcgtcgg
     3181 gcaccggaaa gccgagcatg ccgggcagcg cgccaagcgc cgcgctcagc gcgacgaagg
     3241 tgtctgcggg caggatggtg gtctcccaca cgaagccgta gcgccgcgtg gcgagcagcg
     3301 ccagcacgcc cagggtcgca cccgacaggg caccgaacca cagcgcgtgg ttggccgcgc
     3361 ccacgcccca cgcggcacta cggccactgc cgagcaggcc gccgagcgcg agcggcaggt
     3421 cggcggcttc gcggctgcgg tcgagccagc cggtcacggc cagccaggca cgaccgagca
     3481 ggccgccgtg ctgcaaggca ccgccttgtg cgcgtccctg cagcatcagg ctggcgagcc
     3541 acaggagcag gctcagcaga tgcacgccga gcagaccgcc gagggtccac accacattca
     3601 ccggccgcgt gccgtcgccg agcgcacccg cggcggcgcc gaagccgaac acgaaggcga
     3661 gcacgcaggc gagcaccagc agcaggcgcg cccgcccctg ccagcgccgg atcgcctcgc
     3721 gccagccgcc ggcggcttcg agcacggccg cgcggcgcag cacgcgggcc tcgagatcgc
     3781 ccgcgaccgc acgcgcctcg gcgtgcgcgc gcgcctcgtc gagcggcgga tggcgttctt
     3841 catggcggcg gacgagctcg gccagccagc gcagggacag ggagttcggc ggaaatgacg
     3901 tgggcacgcg gggtccggac gaaaggtcgg cgaagtcggg aagcggaagg ataacaacct
     3961 cgccggcgaa gcgcatggac tcgaccgcct gtgcacggat ccggcgccga tccgccatgg
     4021 caaatgggtt gagccgggcg agaattgatc tcaatcaata aacacccagc caaaatcgtt
     4081 agccttgtgg cttccgccca ccaccggacg cgcccatgag taccgacctg ttccattgga
     4141 ccgacgagtt cagcatcggc ctgcaggaga tcgacgagca gcacaaggag ctcgtcgagc
     4201 tgctcaacca gctccacgtc gcgatcaagg aacaccacgg ctcgaccacc tcgcgccaga
     4261 tcctcgacaa gctcgccgac tacacccgca cccattttgc ggtcgaggag agcctgatgc
     4321 gggtgtcgaa ttaccccgaa ttcgccaccc acaagcagaa ccacgaggac ctgatcgccc
     4381 aggtgcaggc cctgcaggac aagctcgact ccggccaggc gaccatcacc ttcgaactcc
     4441 tgcacttcct caaggtatgg ctcacgcgcc acatcaacga ggcggacaag cgcttcggcg
     4501 cgtatttcgt cgaggccggc ggagccagca agtggtccga tcacgtcgag caggcgatga
     4561 agaagcgcaa gtggtggtgg aagttctggt aagaacggca ggccaggcct cagactcctc
     4621 cacaggcaac ccgactcacc cttccggaaa cacgacgatg cagcacactt ccacggtccg
     4681 actcgccctc ctcgccctcg tcctcgccgg cagcctcggc gccaccgccg cacacgccga
     4741 agcgctcgcc tgccccacgc tgtccacggc gacccaggtc cagccctgcc cgaccgagga
     4801 cgaactcaag tacacctaca ccggctactg cagcgacaac gcccgcctgt acggccgcga
     4861 cgtgctcacc tgcgcgagct tcgagaacta caaggaagtg aagaacgtcg cactgtggga
     4921 ggccgagggc ggcaagttcg acggctacct gtcctgcaac gtcgacgcgg cctcgatcaa
     4981 ggcctcgaag gcacagaagc tgagcgtcga gcgcgtcaag acgctcaccc gcgtgatctg
     5041 cgactacgag aacgaccacc gcctcgtgca tcgcaccaag tcggagtgcg tggtggaggc
     5101 cgcggactgc agcggcgccg agtgcaaggc gcgctgcgac tgagtaagcc gagccggccg
     5161 cgggaccgcg acgcaggcgg cggggccgga gctcgaacgc gggcggcggc ggattcgtcc
     5221 cttcagtccg tgcggcgcgg cagctgcccg gtgaagctgc ccaggatgga gatcacctcg
     5281 tcctcggcga gctcgcgcac gatgaacacc agccggctgc ggcggtcgtc gtagggcgcc
     5341 gaatccggcc aggccggcag gctgctcgcc ggatagacgc tgtgctgcac gcactgcagc
     5401 acccgcggca gcgggtcgcc ggcgacgttg agcaggccct tgatgcgcag gatgcgtccg
     5461 ccgtagacct gcagcagcaa ggcgagcccg tcggagaagc cgaaccagtc gagcggctcg
     5521 tcgaagacga gcacgaaggc gctcacgccg ggggtgtggc gcgagttctc ctgctccgcg
     5581 gccgtgagag agtgaacgcc ggccccgctc cggcccggcc tgcgccaaag cggcgcaacg
     5641 ggcgcacgac gcgcggcgcg caccgcctcc tcgcccagcc aggcggccac gtcgggggtc
     5701 ttgccagcgg ggtcgtacag accgcagccg aacaccgcct ccggccccac ctccccgccc
     5761 gccacctcga tctggcgcgc gccggggttg agcgcggcca ccagcgcacc cacctcggcg
     5821 cgcatcgcgt cggcggccag atcgcccttg gtcagcagca cccggtcggc catcgcgacc
     5881 tggcgcaccg cctcgttgtg gtgggcgagc tgttcgcgcg catgggtgag gtcgaccgcg
     5941 gtcaccacgc cgtcgagccg gtagcgctcg gacaggaagg gctcggccat cagggtgtgg
     6001 atcaccggcg cggggtcggc gagcccggtg gtctcgatca gcacccggcg caggcccttc
     6061 agctcgcgcc gcagcgcacg catgaagagg ctcttgagcg cgcgcaccag gtcgccctgc
     6121 acgctgcagc agatgcagcc cgagtcgagc acgaccaggc tctcgtccac cttctccacc
     6181 agatggtggt cgaccccgac ggcgccgaac tcgttgatca gcaccgcgct gccctccatc
     6241 tgcggctggc gcagcaggtg gttgagcagc gtggtcttgc cggcaccgag gaagccggtg
     6301 aggagggtca ccggaatgcg gcggtcgacg tcggcggatt gcgcgggaat gttcatggcg
     6361 ccgcagtgta gccggcgcac ccgacgatca gaagcctgcc gcgaatccga gcgccgatcg
     6421 cgacttgcgt cgctcctaca gggccactgc aagcgcggtg tttcttgtag gagcgacgca
     6481 agtcgcgata cggcgctgca gatggcaacc gatgccgccg agtacatggg cgctgcgtcg
     6541 agactctcgt cgcgcctacg ggcggccgga tcgcggtggg tgggaagggg cggtcactgc
     6601 cagccgccgc cgagcgcctt gtagagttcg acgcggttgg tcgcctcgat gaggcgcacg
     6661 ccgatcagct ccagttcggc tgcgtagagg ctgcgctggg cgtcgagcag gctcaggtag
     6721 ctgtcgacgc caccgcggta gcgggcgtcg gacagcttga agctgttctg cgtcgccgcc
     6781 acctgcttgc gccgggcgtc gatgcgctcg gcgacggtcg cgcgctcggc aagggcgtcg
     6841 gccacttccc tgaacgccga ctggatcgcc ttctcgtact gggcgacgtt gatgtcgcgc
     6901 tggatctcgg cgagatccag gctggcctgg aggcggccgg cctcgaagat cggcaggcgc
     6961 aactgcggca cgaaactcca cgcccccgag ccgccgtcga acaggccatc gagcgtgctg
     7021 ctcgcggtgc cggcgctggc ggtgagcgtg atgcgcggga agaaggctgc acgcgcagcg
     7081 ccgatgctgg cattggccgc gagcagggca cgttcggcct gcacgatgtc gggacggcgc
     7141 gtgagcacct cggagggcac cccggcgggg agctccgcca ccgtcgcaac cgcctgctcg
     7201 agcccgtccg gcaacagcgt gtccgggaca ccgccgccga ccagttgcgc gagcacgttg
     7261 cggtcctgcg caaccacggt ctcgaaacgt gccgcatcgg cacgcgcgct ctgcagcagg
     7321 gtctcggcct gatgcacgtc gagcgccgac actgccccca gctccaagct gcgccgcacg
     7381 agatcgaacg acgcctggcg ggtctgccgg gtgttgtgcg ccagcgccag gcgttcgcgg
     7441 tctgcagcca ggcgaagcca ggcattggcg acttcggcca ccaggctgat ctgcgcgctg
     7501 cggcgagcct cctcggtcgc gaggtagttc tgcagcgcct gctcctcgag gctgcggacg
     7561 cggccgaaga aatcgagctc gtagctggcg aagccgaggt tggccgagta ctggcggctg
     7621 acgttggagt cacccgtcat gctcaggtcg cccggcacgc gctgcgcact ctgcccgccg
     7681 ctgaccgcga tcgacgggaa caggtccgcc tgctggatgc cgtactgcgc gcgcgcgcgc
     7741 tcgatgttga gcgcggcgac gcgcaggtcg cggttgttct cgagtgcgag cgcgatcacc
     7801 tcgcgcaggc gctgatcggc gaagtagtcg cgccaggcga tggtgtcggc caggggcgcc
     7861 accaccgggg ccgcggtcgg cgtcgcctgc gggaaggtcg ccggcaccgg cgccgcgggg
     7921 cgctcgaaca ccgggatcat cgagcaggcg cctagcgtgg cggcggcgag cgccaccacc
     7981 aggctgcgca gcggcgtggc tgcacgcccg cgacggacgc tcgacaccgg caccgaatcc
     8041 ggcacgagca gggcttttgc gtgggtcttc atctcaacgt ccctcctggg cgatcggcgc
     8101 cacggcctca cccttgggct tgtccgggaa aatgcgcttg atcacgacgt agaacagcgg
     8161 cacgaagaac aggccgagca gcgtcgcgcc gatggtgccg ccgagcacgc cggtgccgat
     8221 cgcgttctgg ctgccggcgc cggcgccggt ggcgatcgcc agcggcagca cgccgaagcc
     8281 gaaggcgagc gaggtcatga tgatcgggcg cagacgcatg cggaccgcgg tcagcgtggc
     8341 ggagatcagg tccttgccct cctccatctg cgccttggcg aactccacga tcaggatcgc
     8401 gttcttcgac gacaggccga tggtggcgag caggccaacc tggaagtaca catcgttaga
     8461 cagcccgcgt ccggaggcgg cgagcagcgc gccgagcacg ccgagcggca ccaccagcat
     8521 caccgcgaag ggcaccgacc agctctcgta cagcgccgcc aggcacagga acaccaccag
     8581 cagcgagagc gcatacagcg caggcgcctg cgagccggaa cggcgctcct cgtacgaggt
     8641 gcccgaccag tcgtagccga tgcccggcgg cagtttggcc atgatctcct cgaccgcctg
     8701 catcgcctcg ccggaggaca agccgggcgc ggcctgtccg agcagctcca tggagggctg
     8761 gccgttgaag cgctccaggc gcggcgagcc gtaggtccag tgcgcggtgg cgaaggccga
     8821 gaacggcacc atgtcgcccg ccttgttgcg cacgtaccac ttgtcgaggt cctcgggcgt
     8881 catgcgcgag cccgcgtcgc cctgcaggta caccttcttg atgcggccgc ggtcgaggaa
     8941 gtcgttgatg tagctgccgc cccaggcggt cgacaaggtg tcgttggtcg gcgatcgaca
     9001 gcccgagcgc gccggccttg gcgtggtcga tgtcgagctt gagttcgggc atgtcctcct
     9061 ggccgttggg ccgcacaccg accaggcgct tgtcctgcgc cgccatgccg aggaactggt
     9121 tgcgcgcagc gagcagggcc tcgtgaccca aaccggagcg gtcctgcatc tgcacggtga
     9181 agccgcccga ggtgccgagc tcgatcaccg ccggcggcac gaaggcgaac accatcgcct
     9241 cgcggatctg cgcgaacgcg cccatcgcgc gcccggccac cgacttcacg tccttgcccg
     9301 gcgccttgcg ctcgtcccag tgcttgaggt tcacgaagcc gatgcccatg tgctggccgc
     9361 tgccgccgaa ggaaaagccg gcgaccgtga agatcgcgcg cacggtgtcc ttttcgttct
     9421 cgaggaagtg gtgctcgacc ttcttcagca cctcgagcgt gcgctcctgg gtggcgccgg
     9481 cgggcagcat gacctggttg aacatcacgc cctggtcttc ctcgggcagg aaggaggtcg
     9541 gcatgcgcac gaagagcagg ccgagcaccg cgacgatggc gaggtagatg gcgaggaagc
     9601 gcacgctgcg ctggaggatg ccgcccacgc tgcgctcgta gcgctgggtg ccgttgtgga
     9661 acttgaggtt gaaccagtgg aagaaccggc cgaacaggcc gccttcgcca tgttcgtggc
     9721 ccttttgcac cggcttgagc atggtggcgc acagcgccgg ggtgaacacg atggccacca
     9781 gcaccgacag gcccatcgcc gacacgatgg tgatggagaa ctggcggtag atcacgccgg
     9841 tcgagccgcc gaagaaggcc atcggcacga acaccgccga cagcaccagg ccgatgccga
     9901 tcagcgcgct ggtgatctgg tccatcgagc gcttggtcgc ttccttgggc ggcaggccct
     9961 cctcggtcat cacgcgctcg acgttctcga ccaccacgat ggcgtcgtcc accagcaggc
    10021 cgatcgccag caccatgccg aacatggtca gcgtgttgat cgagaagccg aaggccgcca
    10081 tcaggccgaa ggtgccgagc agcaccaccg ggatcgccat ggtcgggatc agcgtggcgc
    10141 ggaagttctg caggaacagg tacatcacca ggaagaccag caccaccgcc tcgatcagcg
    10201 tcatcaccac cgattcgatg gagatcttca cgaagggcgt ggtgtcgtag ggcacgacgt
    10261 actgcacgcc ttcggggaaa tagcgcttca tctcgtcgag cttggcgcgc accgcggtcg
    10321 cggtgtcgag cgcgttggcg cccgaggcga gacggatgcc gacacccgca gcgggctggc
    10381 cgttgaagcg ggcgacggtg ccgtagttct cggcgccgat ctcgatgcgc gcgacgtcct
    10441 tcaggcgcac ctcgccgccc tgctggctgc tgcgcagcag gatgtcctcg aactcggcga
    10501 cggtctgcag ccggctctgc gcggtgatgg tggcggtgaa gccctg
//
LOCUS       DAOQBS010000109        10493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_5613_length_10493_cov_15.613815, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000109 DAOQBS010000000
VERSION     DAOQBS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5613_length_10493_cov_15.613815"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKN25_11720"
     CDS             complement(<1..411)
                     /locus_tag="PKN25_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin"
                     /protein_id="HNV91609.1"
                     /translation="MRSRRRFLATSLAAGVLAAHRSAFAHGKAEHPHKAASDAPKEQQ
                     EWGIAGDERSVARTIPIVMTDAMRFVPDRIAIRLGETIRFTHENRGTVMHEMVVGTPA
                     TLAEHAELMRRFPGMEHDEPWMAHVGPGGRGQMIW"
     gene            complement(572..1036)
                     /locus_tag="PKN25_11725"
     CDS             complement(572..1036)
                     /locus_tag="PKN25_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91610.1"
                     /translation="MKLRLFRLAGLMPVAALAAALHGSPVLAQTTEQAQPWPPAASGP
                     ALQARMKSIRAETDPERRMALMEEQIKALEAASQGTPAGCPMMGGQMQGGPMGAGPMM
                     KGAGAGPGAGGMGGMMMDPRLVQEHMKMMQEHMGMMQNMMKMHPAVKPAMPN"
     gene            1223..1903
                     /locus_tag="PKN25_11730"
     CDS             1223..1903
                     /locus_tag="PKN25_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HNV91611.1"
                     /translation="MKILIVEDEVKTGDYLRQGLTEAGFVVDLARDGLDGLHLALSGD
                     YDLIVLDVMLPSLDGWGILQTLRRSGRENPVLFLTARDQIEDRVRGLELGADDYLVKP
                     FAFSELLARVRTLLRRGRSHEPDTLRSANLELDLLRRRVTRCGQRIDLTAKEFALLEL
                     LLRRKGEVLPRSLIASQVWDMNFDSDTNVIEVAVRRLRVKVDDPFEPKLIRTVRGMGY
                     VLEAMEPS"
     gene            1906..3309
                     /locus_tag="PKN25_11735"
     CDS             1906..3309
                     /locus_tag="PKN25_11735"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HNV91612.1"
                     /translation="MIRIHSLTARLATLFAVLAASLLVLVAVLFGRMLDAHFQELDMH
                     ELQGKVTLIRNTLQDEAASGGRAERIQALERSFVGHESVGVLLRDVDGRVLNVIHPEH
                     FTAAQRAGEPPSGVVTDWTVDGRPHRGVEVGIALPRSGSKDQTIAALVALDLSHHVHF
                     LASVRNATWAGVVAAAMAAALFGWFAAHRGLAPLRRVTETARRLSARQLAERLSIDDA
                     PLEVRDHAEAFNGMLARLEAAFQRLGDYSADIAHELRTPISNLMTQTQVALSRPRTID
                     EYQDILASNLEEYERIARMVSDMLFLAKAEENTLAHASEPVDLALEADALIDFYEALA
                     EERQVRIVREGQASVQGDRLMLRRALSNLISNALRHTPDEGQITIRIDADATDIRLAV
                     CNVGDPIPGDQIERIFERFHRGSAQRESRGEGAGLGLAITRSIVQAHGGQISAQSAEG
                     ATCFTITLPGHRAPSSS"
     gene            3771..4421
                     /locus_tag="PKN25_11740"
     CDS             3771..4421
                     /locus_tag="PKN25_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91613.1"
                     /translation="MTILTPAQRRATCIHEAGHAVVFALGGVSVYRLAVAAEGAEAWR
                     TDIRNGRMCTGLWGLCEKADLVLPRQFLRWLMNEGGLHPDGTGYEHLLAHPEGRAQIE
                     AFTTSQQREIRAQMVGLMAGPAAEQRFTDGEARLCRGSALHEVRKAEDLSWLLPGRDD
                     FEHAAELTALTLRRAEVWAAVEHLADALERAGTLAHGIRALLPAALPGWPPRGASA"
     gene            complement(4670..5890)
                     /locus_tag="PKN25_11745"
     CDS             complement(4670..5890)
                     /locus_tag="PKN25_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HNV91614.1"
                     /translation="MSLPLRSLLRILPHAPEGDPGGGGRPRSGLSLPPIEDIARETLP
                     PQDAEPAEDAELAPLADAFVRDALAAGEEALQRQREAVDTMGIELQRQAAWRSEMLQA
                     PIRKLAHQGDEGGPVAQALLALRDKMQDLDPARQKLSGEGLSRALSFIPGVGKPMQRY
                     FQKYEKAQDALDAIIRDLQGGADMLRRDNLTLADDQQSLRAILAQLERQVELGRMIDR
                     RLAREAASASLPAPRRAFVEEELLFPLRQRVIDLQQQVAVSQQGVLALEVVIRNNREL
                     IRGVDRAINVTVSALNVAVTVALGLANQRLVLDRVGALNETTSALIAGTAQALRTQGV
                     DIQTRASSSMLDMGRLEQAFGDVLGAIDDLSRYRRESLPKLDAQIDRLAALARQGAQA
                     IRRMDEGNRAEPQP"
     gene            complement(5907..6836)
                     /locus_tag="PKN25_11750"
     CDS             complement(5907..6836)
                     /locus_tag="PKN25_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HNV91615.1"
                     /translation="MFEFLQGIAFGLLVSFVPWFMAGMYDPRLVVPEELASRWQVILR
                     YAVAMPSIATVLLLTSLWGGFGASLGGWLAGLAAAPAALFLERRWRRWRQARAGKERA
                     AARERARTELARERRERDLLTLDPDFVPADADAVVMQLWEVKRELVGLRRPDLAVQAD
                     RLFTRYHRALDVLDEKFDRREVTYERARGLVGEVGSGAAAGLGAMVALVQGSVGLDAD
                     FVRERLEREAARIGGTEREALQRRLALADETERRLGEIAAGNEAALTALDHVAVAVSR
                     IDTGRGQPAGATEQALQELRRFAERAGLYGRQQ"
     gene            complement(6907..7539)
                     /locus_tag="PKN25_11755"
     CDS             complement(6907..7539)
                     /locus_tag="PKN25_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNV91616.1"
                     /translation="MKLTSQSFTDGARIPGEFAFCIPADEGHVCLGGNRNPQLSWADV
                     PAGTKSFVLVCHDPDVPSQGDDVNQEGRTVPASLPRVDFFHWVVVDLPAELRAIDAGH
                     FSAEVTPGGKPGPEAALGARHGVNDYTAWFAGDEAMKGDYHGYDGPCPPWNDEIVHHY
                     VFTLYALDVARCAVEGRFTGPDVRAAIAGHVLAEAKLTGTYSLNPALAAA"
     gene            complement(7584..9425)
                     /gene="sppA"
                     /locus_tag="PKN25_11760"
     CDS             complement(7584..9425)
                     /gene="sppA"
                     /locus_tag="PKN25_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNV91617.1"
                     /translation="MIRGLFRFLLNVLRMFVRALDLVVRGVFYALLIFGLGVLVSFFF
                     HPEPEVQAGSALVLRPVGTIVEQAELEPPLALLRAGGAPAGQLRLADLVDAVRKARDD
                     ARIAALVIETDELVGGGFSKLAELRAAIADFKASGKPVLARGERFTQSQYYLASVADE
                     LHLSPDGFVLLRGLARYGTYFRDALDKLGVKVHVFRVGEYKSFSEPFTRSDMSDEDRE
                     ATRDLLDGLWRFMRDDIAASRKLAPAAIDAHVNDIRGALAAAGGDAAKAALAAGLVDR
                     FSTRDEWRARLIEAVGTDHEGKDVRTIEAEAYLALAADDTRHAAGSVAVIVAQGTIVD
                     GAEPAGVVAGDTFARLIREAREDEDIKALVLRIDSPGGSAWASELIRRELELTRQAGK
                     PVIASMSSVAASGGYWIATGADEIWAAPSTVTGSIGIFGLFPEFSEPLRRLGIGVDGV
                     ATAPLAGALDPRRPLDPAAAEAMQLGIEHGYRRFLEVVAQARKMTVAEVDAVARGRVW
                     TGEAASGLGLVDKLGSLDDAIAAAAARAGLAEHQVVWPAAGESLEQRVLRRLLRTGED
                     LGIDLAGRSAPAAPLAAAAADVERAARALLRWNDPRHHYLHCLCDAP"
     gene            complement(9451..10194)
                     /gene="trmB"
                     /locus_tag="PKN25_11765"
     CDS             complement(9451..10194)
                     /gene="trmB"
                     /locus_tag="PKN25_11765"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNV91618.1"
                     /translation="MSEVPDEPVAGVEIIGRDSPEHRFSSRSIRSFVLRQGRMSEAQQ
                     RYLDDMLPKVGIDYRAAPLDLDEAFGRKAPKILEIGFGMGETTAKIAAAMPDRDFLGI
                     EVHTPGVGALCKLIAEGHLGNLRIMQHDAVEVMRDMIGDGALAGVHLFFPDPWRKKRH
                     FKRRIVQPDFVALIARKLAPGGYFHCATDWEDYAHWMLGILSAEPALENTADGFAPRP
                     EHRPLTKFENRGLKLGHGVWDLVFRRRAG"
     gene            complement(10191..>10493)
                     /locus_tag="PKN25_11770"
     CDS             complement(10191..>10493)
                     /locus_tag="PKN25_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNV91619.1"
                     /translation="GSGMGILNPWNLRLIIDNAKVPVLVDAGVGTASDAAIAMELGCD
                     GVLMNTAIAAAGKPVLMASAMRKAVEAGREAFLAGRMPRKFYTADPSSPTQGLIGS"
BASE COUNT         1552 a         3618 c         3750 g         1573 t
ORIGIN      
        1 ccagatcatt tgcccgcgtc cacccgggcc gacatgtgcc atccacggct cgtcatgctc
       61 catcccgggg aagcgccgca tcagttcggc gtgctcggcg agcgtcgcgg gcgtgccgac
      121 gaccatctcg tgcatcaccg tgccgcgatt ctcgtgggtg aagcggatcg tctcgcccag
      181 gcgtatcgcg atacggtcgg ggacgaaacg cattgcatcg gtcatcacga tggggatcgt
      241 tctcgcaacg gatctctcat cgcccgcgat tccccattcc tgctgttcct tgggggcatc
      301 ggacgcagcc ttgtggggat gctcggcctt gccatgggcg aacgcgctgc ggtgagcggc
      361 aagcacaccg gccgcaaggc tggtggcgag gaatcgacga cggcttctca tggggatctc
      421 catctgcctg gcaggtgaat cagcaccacg gccacgccca cccctccacc atggtggatg
      481 acctgtctct gcatggggaa atccgtttgc tgatcttgtc ctgagccgga cgcggggcga
      541 tcgacgaggt cgccgccccg cgtctcgagg ctcagttggg catcgccggc ttgaccgccg
      601 gatgcatctt catcatgttc tgcatcatgc ccatgtgctc ttgcatcatc ttcatgtgct
      661 cctgcacgag cctcgggtcc atcatcatgc cgcccatccc tccagccccg ggtccagccc
      721 cggcgccctt catcatcggg ccggcgccca tcggtcctcc ctgcatctgg ccacccatca
      781 tcgggcaccc ggcgggcgtg ccctgcgatg ctgcctccag ggccttgatc tgctcctcca
      841 tcagggccat gcgacgttcg gggtcggtct cggcacggat cgacttcatg cgggcctgaa
      901 gtgcggggcc actcgctgcg ggcggccagg gctgcgcctg ctcggtcgtc tgagcgagga
      961 ccggactgcc gtgcagggct gcggccagag cggcaacggg catgagcccg gcgagacgga
     1021 ataaacgcag tttcatgatc gatctcccat cggttgcggg gttccgtcgc gatacggaac
     1081 gatgtgccac gcagggcatg ttgcgcccgc ccgcctgtcg tcggcctgac ctggacatta
     1141 catttctgta attccgcctg ctctcccgca ccccggaggc agaatcggtc accggcagca
     1201 cgaaccgagt aggagcgaaa caatgaagat cctgatcgtc gaagacgaag tgaagaccgg
     1261 cgactacctg cgccagggcc tgaccgaggc cggattcgtc gtcgatctcg cgcgcgacgg
     1321 cctcgatgga cttcacctcg cgctcagcgg cgactacgac ctcatcgtgc tggacgtgat
     1381 gctgccgtcg ctggacggct ggggcatcct gcagacactg cgtcgcagcg gtcgcgagaa
     1441 cccggtgttg ttcctgaccg cccgtgacca gatcgaggac cgcgttcgtg gcctcgagct
     1501 tggcgccgat gattacctgg tcaagccctt cgcattctcc gagctcctcg cccgggtccg
     1561 caccctgctg cgcagggggc ggagccatga accggacacc ctgcgctcgg cgaatctcga
     1621 actggatctg cttcgccgcc gcgtgacgcg ctgcgggcag cgcatcgacc tgaccgccaa
     1681 ggaattcgcc ctgctcgaac tgctgctgcg gcgcaagggt gaagtgttgc cgcgctcctt
     1741 gatcgcctcc caggtgtggg acatgaactt cgacagcgac accaatgtga tcgaggtcgc
     1801 tgtacgcagg ctgagggtga aggtcgacga ccccttcgag cccaagctga tccgcaccgt
     1861 gcgcggcatg ggctacgtgc tcgaagcgat ggagccgagc tgacgatgat ccgcatccac
     1921 tcgctgacgg cgcgcctggc gacgctgttt gccgtcctcg ccgcgagcct gctcgtgctg
     1981 gtggcggtac tcttcggccg catgctggac gcgcatttcc aggagctgga catgcacgag
     2041 ctgcaaggca aggtcacgct gatccgcaac acgctgcagg atgaggctgc gagtggcgga
     2101 cgcgcggaac gcatccaggc gctcgagcgc tccttcgttg gacacgaaag cgtgggggtg
     2161 ctgctacgcg acgtggacgg gcgcgtgctg aacgtcatcc atcccgagca cttcaccgct
     2221 gcgcagcggg ctggagaacc cccatcgggc gtggtgaccg actggacggt cgatgggcgc
     2281 ccccatcgag gggtcgaggt cggcatcgcg ctgccgcgca gcggctcgaa ggaccaaacg
     2341 atcgcagcgc tggtcgcact cgatctgtcg caccacgtcc atttcctcgc gtcggtgcgc
     2401 aacgccacct gggcgggcgt ggtcgcggca gccatggcgg ccgcgctctt cggctggttt
     2461 gcggcgcacc ggggcctcgc tcccctgcgc cgggtcaccg aaaccgcccg ccggctgtcg
     2521 gcacggcaac tcgccgagcg tctctccatc gacgatgcac cgctggaggt gcgcgaccac
     2581 gccgaggcgt tcaacggcat gctcgcccgg ctcgaggccg cgttccagcg cctcggcgac
     2641 tattcggccg acatcgcgca cgaactgcgc acgccgatct cgaacctgat gacccagact
     2701 caggtcgcgc tatcgcggcc acggacgatc gacgagtacc aggacatcct ggcgtcgaac
     2761 ctcgaggagt acgagcgcat cgcgcgcatg gtcagcgaca tgctgtttct cgccaaggcc
     2821 gaggagaaca cactggcgca tgccagcgaa cccgtggacc tggcgctcga ggcggatgca
     2881 ctcatcgatt tctacgaggc cctggccgaa gagcgccagg tccgcatcgt tcgggagggg
     2941 caggcgagcg tccagggtga tcgactgatg ctgcggcgcg ccttgtcgaa cctgatttcg
     3001 aacgccctgc gccacacgcc ggacgagggg cagatcacga tcaggattga cgcggacgcc
     3061 accgacatcc gcctcgccgt ctgtaatgtc ggcgatccga tccccggcga ccagatcgag
     3121 cggatcttcg agcgcttcca tcgcggcagt gcccagcgcg aatcccgcgg tgaaggcgca
     3181 gggctcgggc ttgccatcac acgctccatc gtccaggccc acgggggtca gatcagtgca
     3241 cagtcggctg aaggtgccac atgcttcacc atcaccttgc ccgggcaccg ggcgccatcg
     3301 tcttcttgat ccagcgaatt cggcacgggt tcgggcgttc tgactggctg caacgagggg
     3361 cccctccctg cggacgacag ccgacgactt ctctgcgagt ccccggagtc agccggacgg
     3421 gcttgatcga gtggggattc aggacctttt tccacgatgc gcgggtgggc gagccgaaag
     3481 cgccgcagat ccgccggacc gtggcttcgc cgcagcgcgc gcgctcgagc aggctttcga
     3541 cgaccccggc ctgggcgtca tccgcctcga tggaacggcg aggcgtctgg cgggttcgtc
     3601 ggcctcgacc ctgacgagtg cgttcggctg catggatcac ccaaggaccc ggaccatgcg
     3661 ccgggcatgg agcgcgcatc cccgctaccg agcacccaca tgatgggcaa agcttcgacc
     3721 tgccgccgcg atcctccggc ggcctccagc atcccgcatg gaacacgaac atgaccatac
     3781 tgacccccgc gcagcggcgc gccacctgca tccacgaggc cggccatgcg gtggtgttcg
     3841 cgctcggcgg cgtgtcggtc taccggctgg cggtggcggc ggaaggggcc gaggcctggc
     3901 gcaccgacat ccgcaacggg cgcatgtgca ccggcctgtg gggcctgtgc gagaaggcgg
     3961 acctggtgct gccgcggcaa ttcctgcgct ggctgatgaa cgagggcggc ctgcatcccg
     4021 acggcacggg ctacgagcac ctgctcgcgc atcccgaggg gcgggcacag atcgaggcct
     4081 tcaccacctc gcagcagcgc gagatccgcg cgcagatggt gggcctgatg gccggaccgg
     4141 cggccgagca gcggttcacg gatggggagg cgcgcctgtg caggggcagc gcgctgcatg
     4201 aagtgcgcaa ggcggaggac ctgtcctggc tgctgccggg gcgcgacgac ttcgagcatg
     4261 ccgccgaact gaccgcgctc accttgcgtc gggcggaggt gtgggcggcg gtcgagcacc
     4321 tggccgacgc gctcgaacgt gccggcacgc tcgcgcacgg catccgtgcg ctgcttcccg
     4381 ccgccctgcc cggctggccc ccgcgcggcg catcggcgta gcgctcttcc tgcctgtcgc
     4441 cccggcggca acaccgcggc gaccgcggcc cgaagaagcg atcgccgcaa tcgcgccttg
     4501 cggcgctcct acaggaagcg cggcacccgg tagcgaaccg ggcgtgcgga gggaacagcc
     4561 cgcgtgcgag cctgcggcgc tcctacagga agcgtcgcgg cgcaccggag cgacggaagc
     4621 ggcaagccgg gcggtggtgt tcatgcccgg ggacaaaacg agcgccggct taaggctgcg
     4681 gctcggcgcg gttgccttcg tccatgcgcc ggatggcctg cgcgccctgg cgcgccagcg
     4741 cggcgaggcg gtcgatctgg gcgtcgagct tgggcaggga ctcgcggcgg tagcgcgaca
     4801 ggtcgtcgat ggcaccgagc acatcgccga aggcctgttc gagccggccc atgtcgagca
     4861 tggacgacga ggcgcgggtc tggatgtcca cgccctgagt gcgcagggcc tgggcagtgc
     4921 cggcgatgag cgcggaggtg gtctcgttga gcgcgccgac gcggtcgagc acgaggcgct
     4981 ggttggccag cccgagcgcg acggtgacgg cgacgttgag cgccgagacg gtgacgttga
     5041 tcgcgcggtc cacgccgcgg atgagctcgc ggttgttgcg gatcaccacc tcgagcgcga
     5101 gcacgccctg ctggctcacc gccacctgct gctgcaggtc gatgacgcgc tggcgcagcg
     5161 ggaagagcag ctcttcctcg acgaaggcgc gccgcggcgc gggcaggctg gcggaggcgg
     5221 cctcgcgggc caggcggcgg tcgatcatgc ggccgagttc gacctggcgt tcgagctggg
     5281 cgaggatggc gcgcagggat tgctggtcgt cggccagggt gaggttgtcg cggcgcagca
     5341 tgtcggcgcc gccctgcagg tcgcggatga tggcgtcgag cgcgtcctgc gccttctcgt
     5401 atttctggaa gtagcgctgc atcggcttgc cgacgccggg gatgaaggac agcgcgcgcg
     5461 acaggccctc gccgctcagc ttctggcgcg cagggtcgag gtcctgcatc ttgtcgcgca
     5521 gcgcgagcag ggcctgcgcg accgggccgc cttcgtcgcc ctggtgggcg agcttgcgga
     5581 tcggcgcctg cagcatctcg ctgcgccagg cggcctggcg ctgcagctcg atgcccatgg
     5641 tgtcgaccgc ctcgcgctgg cgctgcaggg cctcctcgcc ggcggcgagc gcgtcgcgca
     5701 cgaaggcgtc ggcgagcggg gcgagctcgg cgtcctccgc cggctcggcg tcctgcggcg
     5761 gcagggtctc gcgcgcgatg tcctcgatcg gcggcagcga caggcccgag cgcgggcgcc
     5821 cgcctccgcc cgggtcgccc tcgggggcgt gcgggaggat acggagaagg gatcgcagcg
     5881 gcaggctcat cgggaggttc ctcggttcat tgctggcggc cgtagaggcc ggcgcgctcg
     5941 gcgaagcggc gcagctcctg cagggcctgt tcggtggcgc cggcgggctg gccgcggccg
     6001 gtgtcgatgc gcgacaccgc gaccgcgacg tggtcgagcg cggtcagcgc cgcctcgttg
     6061 ccggcggcga tctcgcccag gcggcgttcg gtctcgtcgg caagcgcgag ccggcgctgc
     6121 agcgcctcgc gctcggtgcc gccgatgcgg gcagcctcgc gctccaggcg ctcgcgcacg
     6181 aagtcggcgt ccagcccgac gctgccctgc accagcgcga ccatcgctcc cagcccggcg
     6241 gcagcgccgc tgccgacctc gcccaccagg ccgcgggcgc gctcgtaggt gacttcgcgg
     6301 cggtcgaact tctcgtcgag cacgtcgagc gcgcggtggt agcgggtgaa caggcgatcg
     6361 gcctgcacgg cgagatcggg ccgacgcagg ccgacgagtt cgcgcttgac ctcccatagc
     6421 tgcatcacca ccgcatcggc gtcggcagga acgaaatcgg ggtccagcgt gagcaggtcg
     6481 cgctcgcggc gttcacgcgc gagctcggtg cgcgcacgct cgcgcgcggc ggcgcgctcc
     6541 ttgccggcac gcgcctgccg ccagcgccgc cagcgccgct ccaggaagag cgcggccgga
     6601 gccgcggcga ggccggcgag ccagccgccc aggctggcgc cgaagccacc ccacagcgag
     6661 gtcagcagca gcacggtcgc gatcgagggc atggccaccg cgtagcgcag gatgacctgc
     6721 cagcgcgagg cgagctcctc cggcaccacc aggcgcgggt cgtacatccc ggccatgaac
     6781 cagggcacga aggagaccag caggccaaag gcgatcccct gcaggaattc aaacatccag
     6841 acgactccgg gtcagcgcga aaatagggga cagaccccaa tttccggggt ctgtccccta
     6901 tttcccttac gccgcggcga gcgcagggtt gagcgaatag gtgccggtga gcttggcctc
     6961 ggcgagcacg tggccggcga tcgccgcgcg cacgtccggg ccggtgaagc ggccctcgac
     7021 cgcgcagcgc gccacgtcga gcgcgtacag cgtgaacacg tagtggtgca cgatctcgtc
     7081 gttccacggc gggcagggac cgtcgtagcc gtgatagtcg cccttcatgg cttcatcgcc
     7141 ggcgaaccag gcggtgtaat cgttgacgcc gtggcgggcg ccgagcgcgg cctccggccc
     7201 gggcttgccg cccggcgtga cctcggcgga gaagtggccg gcgtcgatcg cgcgcagctc
     7261 ggcgggcagg tcgaccacca cccaatggaa gaagtccacc cgcggcaggc tggccggcac
     7321 ggtgcggcct tcctggttga cgtcgtcgcc ctggctcggc acgtcggggt cgtggcagac
     7381 gagcacgaag gacttcgtcc ccgcgggcac gtcggcccac gacagctgcg ggttgcggtt
     7441 gccgcccagg cagacgtgcc cttcgtcggc cgggatgcag aaggcgaatt cgcccgggat
     7501 gcgggcgccg tcggtgaagc tctggctggt gagtttcaag tcatcctcct tgcggtgtga
     7561 tgtgggtacg cgaacgcgcg ctttcaaggc gcgtcgcaca ggcagtgcag gtagtggtgg
     7621 cgcggatcgt tccagcgcag cagcgcgcgg gcggcgcgct ccacgtccgc ggccgcggcg
     7681 gcgagcggcg cggccggcgc gctgcggccg gccaggtcga tgccgaggtc ctcgccagtg
     7741 cgcagcagcc ggcgcaacac gcgctgctcg agggactcgc ccgccgccgg ccacaccacc
     7801 tgatgctcgg cgaggccggc gcgcgcggcc gcggcggcga tcgcgtcgtc caggctgccg
     7861 agcttgtcga ccaggccgag gccgctcgcc gcctctccgg tccacacgcg accgcgggcg
     7921 accgcgtcga cctccgcgac cgtcatcttg cgcgcctgcg cgacgacctc caggaagcgc
     7981 cggtagccgt gctcgatgcc gagctgcatg gcctcggccg cggccgggtc gagcgggcgg
     8041 cgcgggtcga gcgcgccggc gagcggcgcg gtggcgacgc catcgacgcc gatgccgagg
     8101 cggcgcagcg gctcggagaa ctccgggaag aggccgaaga tgccgatcga gccggtcacc
     8161 gtggaaggcg ccgcccagat ctcgtcggca ccggtggcga tccagtagcc gcccgaggcc
     8221 gccaccgagc tcatcgacgc gatcaccggc ttgccggcct ggcgggtgag ttcgagttcg
     8281 cgacggatga gctccgaggc ccaggcgctg ccgcccgggc tgtcgatgcg caacaccagc
     8341 gccttgatgt cctcgtcctc gcgcgcctcg cggatcaggc gggcgaaggt gtcgccggcg
     8401 accacgccgg ccggctcggc gccatcgacg atggtgccct gggcgacgat caccgccacg
     8461 ctgccggcgg cgtgacgggt gtcgtccgca gcgagcgcga gataggcctc ggcctcgatg
     8521 gtgcgcacgt ccttgccctc gtggtcggtg ccgacggcct cgatcaggcg ggcgcgccat
     8581 tcatcgcggg tgctgaagcg gtccaccagg ccggcggcga gcgcggcctt ggcggcatcg
     8641 cccccggcgg cggcgagcgc gccgcggatg tcgttcacat gcgcgtcgat cgccgcgggc
     8701 gcgagcttgc ggctggcggc gatgtcgtcg cgcatgaagc gccacagccc gtcgaggagg
     8761 tcgcgggtgg cctcgcggtc ctcgtcggac atgtcgctgc gggtgaaggg ctcggagaag
     8821 gacttgtact cgccgacgcg gaacacgtgc accttgaccc cgagcttgtc gagcgcgtcg
     8881 cggaagtagg tgccgtagcg cgccaggccg cgcagcagca cgaagccgtc cggcgacagg
     8941 tggagttcgt cggccaccga ggcgaggtag tactgcgact gggtgaagcg ctcgccgcgc
     9001 gccagcaccg gcttgcccga ggccttgaaa tcggcgatcg cggcgcgcaa ctcggcaagc
     9061 ttggagaagc cgccgccgac cagctcgtcg gtctcgatca ccagcgcggc gatgcgcgcg
     9121 tcgtcgcgcg ccttgcgcac cgcgtccacc agatccgcca gacgcagctg gccggccggt
     9181 gcgcccccgg cgcgcagcag cgccagcggt ggctcgagct cggcctgctc gacgatcgtg
     9241 cccaccggtc gcagcaccag cgcggacccg gcttgcacct cgggctcggg gtggaagaag
     9301 aaggacacca gcacaccgag gccgaagatc agcagggcgt agaagacgcc gcgcacgacg
     9361 aggtcgagcg cgcgcacgaa catgcgcagc acgttcaata ggaagcggaa caggccccgg
     9421 atcatgctgc ttgtcctcgt gacggatggt tcagcccgcg cggcggcgga agacgaggtc
     9481 ccataccccg tgtcccagct tcagaccacg gttttcgaat ttcgtcagcg gacgatgctc
     9541 gggacgcggg gcgaagccgt cggcggtatt ctccagcgcc ggctcggcgg agaggatccc
     9601 gagcatccag tgcgcgtagt cctcccagtc ggtggcgcag tggaagtagc cgccgggcgc
     9661 gagcttgcgc gcgatcaggg cgacgaagtc gggctgcacg atgcggcgct tgaagtggcg
     9721 cttcttgcgc caggggtcgg ggaaaaacag atgcacgccg gcgagcgcgc cgtcgccgat
     9781 catgtcgcgc atcacctcga ccgcgtcgtg ctgcatgatg cgcaggttgc ccagatggcc
     9841 ttccgcgatc agcttgcaca gcgcgccgac gccgggcgta tgcacctcga tgccgaggaa
     9901 gtcgcgatcc ggcatcgccg cggcgatctt cgccgtggtc tcgcccatgc cgaagccgat
     9961 ctcgaggatc ttcggcgcct tgcggccgaa ggcctcgtcc agatcgagcg gcgcggcgcg
    10021 gtagtcgatg ccgaccttcg gcagcatgtc gtcgagatag cgctgctgcg cctcggacat
    10081 gcgcccctgg cgcagcacga agctgcggat gctgcggctg gagaagcggt gctcggggct
    10141 gtcgcggccg atgatctcga caccggcgac gggttcgtca ggcacttcgc tcatgaaccg
    10201 atcaacccct gggtcggcga gctcgggtcg gcggtgtaga acttgcgcgg catgcgcccg
    10261 gcgaggaagg cctcgcggcc ggcctcgacc gccttgcgca tcgcgctcgc catcagcacc
    10321 ggcttgccgg cggcggcgat cgcggtgttc atcagcacgc cgtcgcagcc cagctccatc
    10381 gcgatcgcgg catccgatgc ggtgccgacg ccggcgtcga ccagcactgg caccttggcg
    10441 ttgtcgatga tcaggcgcag gttccacggg ttgaggatgc ccatgccgga gcc
//
LOCUS       DAOQBS010000110        10476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_5626_length_10476_cov_16.888494, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000110 DAOQBS010000000
VERSION     DAOQBS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10476
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5626_length_10476_cov_16.888494"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..3701
                     /locus_tag="PKN25_11775"
     CDS             225..3701
                     /locus_tag="PKN25_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNV91620.1"
                     /translation="MSIHVKLNHVTRYRYDRPVALSPQVVRLRPAPHCRTPIHAYALK
                     VDPAGHFINWQQDPQSNYLARLVFPDKATELRIEVDLVAELSVINPFDFFLEPAAEKI
                     PFAYEDALRVELAPYLAKAPAAALGPKFIDCLESIPRAPRASVDFLVALNQRLQQDIR
                     YLVRLEPGVQTPEETLTLASGSCRDSAWLLVQLLRHLGLAARFVSGYLIQLVPDVKSL
                     DGPSGTAVDFTDLHAWCEVYLPGAGWVGLDPTSGLFAGEGHIPLACSPEPSSAAPITG
                     FTDECECEFEHHMKVERVWEAPRVTKPYTDEQWAAIEVLGQRIDADLKAHDVRLTQGG
                     EPTFVALDDRDGAAWNSDALDPHNRDASKPSKRTLAENLMFRLKDHYAPHGLLHFGQG
                     KWYPGEQLPRWSLNCYWRRDGEPIWRKPELFARELSGTAVDEAVAARFLARVGERLGV
                     DTQWMMAAFEDAWYYLWRERRLPANVDPHDARVDDPLERARLAKVFDQGLRQVVGHVL
                     PLVRDHVGADRWQSGPWFLRAERLYLIPGDSPIGYRLPLDSQPWAGKGDLPLIHPADP
                     NQPFPTLPAHAELRQQLRPTGTTLGADSGLGFPFAAHGGGWTPSRVEHAGFHGVQGEA
                     AVPRPGTSALDRRDERTDPARRPAPFESAAWITRSALCAEPRGGLLHLFMPPTAALED
                     YLEIVTAIEDTAAEFKLPVVLEGYEPPSDPRLAHFRITPDPGVIEVNIHPAASWDELV
                     ERTGTLYEEARQARLSTEKFMLDGRHTGTGGGNHFVLGGATPGDSPFLRRPDLVRSLL
                     SYWHNHPSLSYLFSGLFIGPTSQAPRVDEARHDSVHELEVAFQQMPEPGIQVPPWLID
                     RLLRNLLIDASGNTHRAEFCIDKLYSPDSATGRLGLLELRAFEMPPHARMSLAQQLLL
                     RALIARFWQQPYAPGRLRRWGTELHDRFLLPHWVAEDFRDVVGELREFGYPLEFDWFA
                     PHFEFRFPRYGEFAARGVRVELRMALEPWHVMGEEGAPGGTVRYVDSSVERLQVKVSG
                     LNDDRHVLTCNGRAVPLQPTGTVGEFVAGVRYRAWQPPSCLHPTIGVHAPLVFDLVDG
                     WMQRSMGGCVYHVAHPGGRNHESYPVNPYEAEGRRLARFTTTGHTPGRVAPAPATRNA
                     DFPFTLDLRRQG"
     gene            3708..6314
                     /locus_tag="PKN25_11780"
     CDS             3708..6314
                     /locus_tag="PKN25_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNV91621.1"
                     /translation="MQVQPHRPPALLARYVARPDRYDELCQRIGSVVVLRPHWRDFFH
                     GLAAMPADELSARRAALGRQIHENGITYNVYADPRGFARPWELDLLPYMLPAAEWAQI
                     EAAVIQRATLFDRILADLYGEQTLLEQGLVPPALIYGHSGFLRPLVGARPAGGRFLHL
                     YAVDLARSPDGRWWVVADRTQAPSGAGYALENRSVVARVLPELYRAAGVEPLVPFFEG
                     FRDGLVELAPADGAAAGEDPLIVVLTPGPYNETYFEHAFLAREMGFPLVEGQDLTVRD
                     DKVWLRTLEGLRRVHVILRRVDDLWCDPLELREDSALGVAGLVAAVRAGMVTVANALG
                     SGILETGALLGYLPRLCEHLLGSKLRMPSVATWWCGEPAACEYALKHLRELVIKPAYP
                     TLGARPVFCGDLPVAELDALAARIALRPFDFVAQEMVNLSQAPVLADEAGGAGNKAPA
                     RDARALVARNIGLRVFAAAGAEGFRVLPGGLVRVASEADMRIVSMQHGGGSKDAWVLG
                     SPARPRPVPRIVPGQLAPFAGGARVLGLSSRVAENFFWLGRYSERADAAARLGRETLA
                     RLGEAGEASWLGEDGKVTAALQALCRQRGLLATVAGEADAAPTPPAPLAQALFDPAQP
                     GSVVANLRQVLRVAALVRDRLSPDSWRIYNRLSEFAAAPAQAPRLGEALQRLDESLLS
                     LVTLSGFVIESMPRDAGWRFLSIGRRIERLQFLAAALSALLLEPSQGGLKVLLAITDA
                     ELRYRSRHARGLAPQPVAELVMLDGDNPRALRYQLDSLAEHVALLPDGAALAAPLRER
                     LAALDALALCDCFVHELAGARSRAAAQARHCEPLRQVLDDIWRGGNELADALARRYFT
                     HLDARSRATVSL"
     gene            6325..7287
                     /locus_tag="PKN25_11785"
     CDS             6325..7287
                     /locus_tag="PKN25_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNV91622.1"
                     /translation="MALYEILHDTTYRYDSAVALSQQVAHLRPRECAGQRTLAHHLEI
                     APAPARRSERSDFFGNPVTAFALHAPHTELVVRARSRIRVDPPAWPEPAASPPWEAAR
                     DHLRDGLSGHAPLRADARDAGQYRFASPFVALGDEAAQYADYALASFTPGRPVLDALL
                     ALSARIHADFRFDPAATSVATPVAEVFAARRGVCQDFSHLMIACLRALGLAARYVSGY
                     LLTEPPPGQPRLIGADASHAWVALWCPGAGWIDIDPTNDLQPGSGHITLAWGRDYGDV
                     CPLRGVILGGGGHGVEVAVTVMPVEEGPPPRRGGRPARAADAPD"
     gene            7447..8877
                     /locus_tag="PKN25_11790"
     CDS             7447..8877
                     /locus_tag="PKN25_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNV91623.1"
                     /translation="MNAAKPFDEMHAADGTIRTHYQPYSEWLGHTPRELIARKRKEAD
                     LAFHRVGITFNVYGADGGKERLIPFDLLPRIIPGDEWRTLEAGLRQRVRALNAFLADI
                     YHGQEILRAGRIPADQVLDNAQFRPEMKGVHVPGGLYAMIAGIDLVRAAGADGKGDYY
                     VLEDNLRVPSGVSYLLENRKMMMRLFPDLFSRYDVQPVEHYPDLLLETLRAVAPAGVL
                     DPTAVLLTPGAFNSAYFEHSFLAQQMGIELVEGQDLFVEDDTVFMRTTQGPRRVDVIY
                     RRLDDDFLDPEVFRADSMLGVPGLMSAYRAGRVTLANAVGTGVADDKSIYPYVPEMVR
                     FYLGEEPILNNVPTWMLREPDDLAYTLAHLPELVVKEVHGAGGYGMLVGPAATKAEIE
                     EFRQRILAAPEKYIAQPTLSLSTCPTFVDAGIAPRHIDLRPFVLSGGREIRMVPGGLT
                     RVALKAGSLVVNSSQGGGTKDTWVVG"
     gene            8915..9862
                     /locus_tag="PKN25_11795"
     CDS             8915..9862
                     /locus_tag="PKN25_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNV91624.1"
                     /translation="MLSRTADHLYWMARYTERAENTVRMLDVSYRMSLLPGANGGDTE
                     SWSAILDISELSEAFARTGRPLSACDVIEFVVLDRDNPSSVWNCLRAARENAHAVRGT
                     LTAEMWETTNATWLEIREMTPQKLRATDLGEFFEWVKFRSHLLRGVTFGTMLHDEAFK
                     FVRLGTFLERADNTARLLDVKYHSLLPKAEGLTSSTDFYQWGALLRSVSAFETYRHVY
                     RDVITPYKVAELLILNADMPRSLHACLDEINGILGEIASIHSAEAARQAGELHATLHY
                     GRMDDIFDAGLHPWLERFLVRINRLGDSIGENFLVPVAA"
     gene            9927..>10476
                     /locus_tag="PKN25_11800"
     CDS             9927..>10476
                     /locus_tag="PKN25_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNV91625.1"
                     /translation="MTYCVAMCLDEGLVCLSDSRTNAGVDHINTFRKMTVFERAGDRV
                     LVLMSAGNLAITQAVISLLREHAAVQDRDSLWNAPTLFECARLVGETIRGVHRQDAEA
                     LKQFGVEFNASFILAGQIGDESPRLFNIYSAGNFIEASIDTPYFQIGESKYGKPIIDR
                     IVTSKTSLDEAAKCALISMDSTI"
BASE COUNT         1421 a         3944 c         3577 g         1534 t
ORIGIN      
        1 ccacgcctcc gaggcctctt cgccgtagcg acgcaagtcg cgatgccggc gctggcgcgc
       61 tggtgccgca ccgcgctggt gcgctccggg cgcaaatggt gcgcgactgc gcctgcacgg
      121 aaggggcagt gccagcggaa acggcggaag ccaggcgtcg cgcgggcttg cacgcttctt
      181 gctgacaccc ggtgtcgccc cactacgctc cctgcgaggc cgccgtgtcc atccacgtca
      241 agctcaacca cgtcacccgt taccgctacg accgcccggt cgcgctgtcg ccgcaggtcg
      301 tgcgcctgcg cccggcgccg cactgccgca cgcccatcca tgcctacgcg ctgaaggtcg
      361 accccgccgg ccacttcatc aactggcagc aggacccgca gtcgaactat ctcgcccgcc
      421 tcgtcttccc cgacaaggct accgaactgc gcatcgaggt cgatctggtg gccgagctgt
      481 cggtcatcaa ccccttcgat ttcttcctcg aacccgcggc ggagaagatc cccttcgcct
      541 acgaggacgc cctgcgcgtc gagctcgcgc cctacctggc gaaggcgccg gctgccgcgc
      601 tggggccgaa gttcatcgac tgcctcgaat ccatcccgcg cgcgccgcgg gccagcgtgg
      661 atttcctggt cgcgctcaac cagcgcctgc agcaggacat ccgctacctg gtgcgcctgg
      721 agccgggcgt gcagacgccg gaagagacgc tgacgctggc gagcggctcg tgccgcgact
      781 cggcctggct gctggtgcag ttgctgcgcc acctcggcct ggcggcgcgc ttcgtgtccg
      841 gctacctgat ccagctcgtg cccgacgtga agtcgctcga cggcccctcg ggcaccgcgg
      901 tggacttcac cgacctgcac gcctggtgcg aggtctatct gcccggtgcc ggctgggtgg
      961 gcctggatcc gacctccggc ttgttcgccg gcgagggcca catcccgctc gcctgctcgc
     1021 ccgagccctc gtcggcggcg ccgatcaccg gcttcaccga tgaatgcgaa tgcgagttcg
     1081 agcaccacat gaaggtcgag cgcgtgtggg aggcgccgcg cgtcaccaag ccctataccg
     1141 acgaacagtg ggcggcgatc gaggtgctgg gccagcgcat cgacgccgac ctgaaggccc
     1201 acgacgtgcg cctcacgcaa ggcggcgagc ccaccttcgt ggcactcgac gaccgcgacg
     1261 gcgcggcctg gaacagcgac gcgctcgatc cgcacaaccg tgatgccagc aagccgagca
     1321 agcgcaccct ggccgaaaac ctgatgttcc gcctcaagga ccactatgcg ccgcacggcc
     1381 tgctgcactt cggccagggc aagtggtacc cgggcgagca gctgccgcgc tggtcgctca
     1441 actgctactg gcggcgcgac ggcgagccga tctggcgcaa gcccgagctg ttcgcgcgcg
     1501 agctgtccgg caccgcggtg gacgaggccg tggcggcgcg cttcctcgcg cgcgtcggcg
     1561 agcgcctcgg cgtggatacg cagtggatga tggcggcctt cgaggacgcc tggtactacc
     1621 tgtggcgcga gcgccggctg ccggccaacg tcgatccgca cgatgcgcgc gtcgacgacc
     1681 cgctcgaacg cgcgcggctc gccaaggtct tcgaccaggg gctgaggcag gtcgtcgggc
     1741 acgtgctgcc gctggtgcgc gatcacgtcg gcgccgatcg ctggcagagc gggccgtggt
     1801 tcctgcgtgc cgagcgcctg tacctgatcc ccggcgattc gccgatcggc taccgcctgc
     1861 cgctcgattc ccagccctgg gcgggcaagg gcgacctgcc cttgatccac cccgccgacc
     1921 ccaaccagcc cttcccgacg ctgcccgcgc acgccgagct gcgccagcag ctgcggccga
     1981 cgggcacgac gctcggcgcc gacagcggcc tcggcttccc cttcgccgcc cacggcggcg
     2041 ggtggacgcc gagccgtgtc gaacatgccg gcttccatgg ggtgcagggc gaggccgcgg
     2101 tgccgcggcc cggtacgtcc gcgctggatc gccgcgacga gcgcaccgac cccgctcgcc
     2161 gtccggcacc cttcgagtcc gccgcctgga tcactcgcag cgcgctgtgc gccgagccgc
     2221 gcggcggcct gctccatctc ttcatgccgc cgactgccgc gctggaggac tacctcgaga
     2281 tcgtcaccgc catcgaggac accgccgccg aattcaagct gccggtggtg ctcgaaggct
     2341 acgagccgcc gtccgacccg cgcctggcgc acttccgcat cacccccgac ccgggcgtca
     2401 tcgaggtcaa catccacccc gcggcgagct gggacgaact cgtcgagcgc accggcacgc
     2461 tctacgagga ggctcggcag gcgcgcctgt cgaccgagaa gttcatgctc gacggccgtc
     2521 ataccggcac cggcggtggc aaccacttcg tgctcggcgg tgccacgccg ggcgactcgc
     2581 ccttcctgcg gcggcccgac ctggtgcgca gcctgttgag ctactggcac aaccacccca
     2641 gcctgtccta cctgttctcg gggctcttca tcggcccgac ctcgcaggcg ccgcgggtgg
     2701 acgaagcgcg ccacgactcg gtgcacgagc tcgaagtcgc cttccagcag atgccggagc
     2761 cgggcatcca ggtgccgccg tggctcatcg accgcctgct gcgcaacctg ctgatcgacg
     2821 ccagcggcaa cacccaccgc gccgagttct gcatcgacaa gctctacagc cccgacagcg
     2881 ccaccgggcg gctgggcctg ctcgagctgc gcgccttcga gatgccgccg cacgcgcgca
     2941 tgagcctggc ccagcagctg ctgctgcgcg cgctgatcgc gcgcttctgg cagcagcctt
     3001 atgccccggg tcggctgcgg cgctggggta ccgagctgca cgaccgcttc ctgctgccgc
     3061 actgggtggc ggaggatttc cgcgacgtcg tcggcgagct gcgcgaattc ggctacccgc
     3121 tcgagttcga ctggttcgcg ccgcacttcg agttccgctt ccccaggtac ggcgagttcg
     3181 ccgcgcgcgg cgtgcgggtc gaactgcgca tggcgctcga gccctggcac gtgatggggg
     3241 aggagggggc gcccggtggc acggtgcgct acgtcgattc ctcggtggag cgcctgcagg
     3301 tcaaggtgtc cgggctcaac gacgaccgcc acgtgctgac ctgcaacggc cgcgccgtgc
     3361 cgctgcagcc caccggcacg gtgggcgagt tcgtcgccgg ggtgcgctac cgtgcctggc
     3421 agccgccgtc ctgcctgcac ccgaccatcg gcgtgcacgc gccgctggtg ttcgacctgg
     3481 tcgatggctg gatgcagcgc tcgatgggcg gctgcgtgta tcacgtggcg catccgggcg
     3541 ggcgcaacca cgagagctac ccggtgaacc cctacgaggc cgaaggccgc cgcctggccc
     3601 gcttcaccac caccggccac acgccgggcc gcgtcgcacc cgcgcccgcc acgcgcaacg
     3661 ccgatttccc cttcaccctc gacctgcgcc gccagggctg acccgccatg caggtccagc
     3721 cgcaccgccc gcccgcgctg ctcgcccgct acgtcgcgcg gcccgaccgc tacgacgagc
     3781 tctgccaacg catcggcagc gtcgtggtgc tgcggccgca ctggcgggac ttcttccacg
     3841 gcctcgccgc catgcccgcc gacgagctgt cggcgcggcg ggccgcgctc ggccggcaga
     3901 tccacgagaa cggcatcacc tacaacgtgt atgccgatcc gcgcggcttc gcgcgcccgt
     3961 gggagctcga cctgctgccc tacatgctgc ccgcggcgga gtgggcgcag atcgaggccg
     4021 cggtgatcca gcgcgccacc ctgttcgacc gcatcctcgc cgacctctac ggcgagcaga
     4081 ccctgctcga gcagggcctg gtgccgcccg cgctcatcta tggccatagc ggctttctgc
     4141 gcccgctcgt gggcgcgcgg ccggcgggcg ggcgcttcct gcacctgtac gcggtggacc
     4201 tggcgcgttc gcccgacggg cgctggtggg tggtggccga ccgcacccag gcgccctcgg
     4261 gtgcgggcta cgcgctggag aaccgcagcg tggtcgcgcg cgtgctgccc gagctctacc
     4321 gcgccgccgg cgtcgagccg ctggtcccct tcttcgaggg cttccgcgac ggcctcgtcg
     4381 agctcgcgcc ggccgacggc gcggcggccg gggaagatcc cctgatcgtg gtgctcacgc
     4441 ccgggcccta caacgagacc tacttcgagc acgccttcct cgcccgcgag atgggcttcc
     4501 cgctcgtcga gggccaggac ctcaccgtgc gcgacgacaa ggtctggttg cgcacgctcg
     4561 agggcctgcg ccgggtgcat gtgatcctgc gccgggtgga cgacctgtgg tgcgatccgc
     4621 tcgaactgcg cgaagactcc gcgctcggcg tcgccggcct ggtggcggcg gtgcgtgccg
     4681 gcatggtgac ggtggccaac gcgctcggca gcggcatcct cgagaccggg gcgttgctcg
     4741 gctacctgcc gcggctgtgc gagcacctgc tcggcagcaa gctgcgcatg ccctcggtgg
     4801 cgacctggtg gtgcggcgag ccggcggcct gcgaatacgc gctgaagcac ctgcgcgagc
     4861 tggtgatcaa gccggcctac cccaccctgg gtgcgcggcc ggtgttctgc ggcgacctgc
     4921 cggtggcaga gctcgacgcg ctggcggcac ggatcgcgct gcggcccttc gacttcgtcg
     4981 cgcaggagat ggtcaacctc tcgcaggccc cggtgctggc ggacgaggcg ggcggcgccg
     5041 ggaacaaggc gcccgcgcgc gacgcgcgcg cgctggtcgc gcgcaacatc gggctgcgcg
     5101 tgttcgcggc ggcgggggcg gaggggtttc gcgtgctgcc gggcgggctc gtccgggtgg
     5161 cctcggaggc cgacatgcgc atcgtgtcga tgcagcacgg cggcggcagc aaggacgcct
     5221 gggtgctcgg ctcgccggca cggccgcgtc cggtgccgcg catcgtgccc ggccagctcg
     5281 cgccctttgc cgggggcgcg cgcgtgctcg gcctgtcgag ccgggtggcg gagaacttct
     5341 tctggctcgg acgctacagc gagcgcgccg acgctgccgc ccgcctcggc cgcgagacgc
     5401 tggcgcgcct gggcgaggcc ggcgaggcct cgtggctggg cgaggatggc aaggtgaccg
     5461 cggccctgca ggcgctgtgc cggcagcgcg gcctgctcgc caccgtggcg ggggaggccg
     5521 acgcggcacc cacacccccc gcgccgctcg ctcaggcgct cttcgatccc gcccagcccg
     5581 gcagcgtggt ggccaacctg cgccaggtgc tgcgcgtcgc agccctggtg cgcgaccgcc
     5641 tgtcgccgga cagctggcgc atctacaacc gcctgtcgga gtttgccgcg gcgccggctc
     5701 aggccccgcg cctcggcgag gcgctgcagc gcctggacga gtcgttgctg tcgctggtga
     5761 cgctgtcggg tttcgtcatc gagagcatgc cgcgcgacgc cggctggcgc ttcctgtcga
     5821 tcgggcggcg catcgagcgc ctgcagtttc tcgccgccgc gctgtcggcg ctgctgctcg
     5881 agccctcgca aggcgggctc aaggtgctgc tcgcgatcac cgacgccgag ctgcgctacc
     5941 gcagccgcca cgcgcgcggc ctggccccgc agccggtggc cgagctggtc atgctcgacg
     6001 gcgacaaccc gcgcgcgctg cgctaccagc tcgattcgct cgccgagcac gtcgccctgc
     6061 tgcccgacgg cgccgcgctc gccgcgccgc tgcgcgaacg cctggccgcg ctcgacgcgc
     6121 tcgcgctgtg cgactgcttc gtgcacgagc tggccggcgc gcgtagccgc gcggccgcac
     6181 aggcgcgcca ctgcgagccc ctgcggcagg tgctggacga catctggcgc ggcggcaacg
     6241 agctcgccga tgcgctggcg cggcgctact tcacccacct cgacgcccgc agccgggcga
     6301 ccgtgtcgct gtagggcggc ggcgatggcg ctctacgaga tcctccacga caccacctac
     6361 cgctacgaca gtgcggtggc gctgtcgcag caggtggctc acctgcgccc gcgcgaatgc
     6421 gccggccagc gcaccctcgc gcaccacctg gagatcgcgc cggcgccggc ccggcgtagc
     6481 gagcgcagcg acttcttcgg caacccggtg accgcgttcg cgctgcacgc gccgcacacg
     6541 gaactcgtcg tgcgcgcacg cagccgcatc cgcgtcgacc cgccggcctg gcccgagccg
     6601 gcggcgagtc cgccctggga ggccgcgcgc gaccacctgc gcgacggcct ttccggccac
     6661 gcgccgctgc gcgccgacgc gcgcgacgcc gggcagtacc gcttcgcttc gcccttcgtg
     6721 gcgctcggcg acgaggccgc gcagtacgcc gactatgcgc tggcgagctt caccccgggg
     6781 cggccggtgc tcgacgcctt gctcgcgctg tcggcgcgca tccacgccga ttttcgcttc
     6841 gaccccgcgg cgaccagcgt cgccaccccg gtggcggagg tcttcgcggc gcgccgtggg
     6901 gtgtgccagg acttctccca cctgatgatc gcctgtctgc gcgcgctcgg cctggccgcg
     6961 cgctacgtct ccggctacct gctcaccgag ccgccgccgg gccagccgcg cctgatcggc
     7021 gccgacgcct cgcacgcctg ggtggcgctg tggtgcccgg gcgcgggctg gatcgacatc
     7081 gatccgacca acgacctgca gcctggcagc ggtcacatca cgctggcctg ggggcgggac
     7141 tacggcgacg tgtgtccgct gcgcggcgtc attctcggcg ggggcgggca cggcgtcgag
     7201 gtcgcggtga cggtgatgcc ggtggaggag ggcccgcccc cacggcgggg cgggcgcccg
     7261 gcgcgtgccg ccgacgcacc agattgatgc gtcggcggcc accatggtgc gcaccagcgc
     7321 ggtgcccgcg ggcgtcgacg tgcccgaccc cgttgtcggc gcggctttgc gcgtttcatg
     7381 ggcttggcac gctttctgct gtaggccctg cgagggagtc gataaaccat ccgcgcagag
     7441 cacgccatga acgccgccaa gcctttcgat gagatgcacg ccgccgacgg cacgatccgg
     7501 acgcactacc agccctacag cgagtggctc gggcacaccc cgcgcgagct gatcgcgcgc
     7561 aagcgcaagg aggccgacct ggccttccac cgcgtcggca tcaccttcaa cgtctatggc
     7621 gccgacggcg gcaaggagcg cctgatcccc ttcgacctgc tgccgcgcat catccccggc
     7681 gacgagtggc gcacgctgga ggccggcctg cgccagcgcg tgcgcgcgct caacgccttc
     7741 ctcgccgaca tctaccacgg ccaggagatc ctgcgcgccg gacgcatccc cgccgaccag
     7801 gtgctggaca acgcccagtt ccgccccgag atgaagggtg tgcacgtgcc cggcgggctg
     7861 tacgcgatga tcgccggcat cgacctggtg cgtgccgcgg gtgccgacgg caagggcgac
     7921 tactacgtgc tggaggacaa cctgcgcgtg ccctcggggg tgagctacct gctggagaac
     7981 cgcaagatga tgatgcggct cttccccgac ctgttctcgc gctacgacgt gcagccggtc
     8041 gagcactacc ccgacctgct gctcgagacc ctgcgcgcgg tggcgccggc cggcgtactc
     8101 gaccccaccg cggtgctgct caccccgggc gccttcaaca gcgcctactt cgagcacagc
     8161 ttcctcgccc agcagatggg catcgagctc gtcgagggcc aggacctctt cgtcgaggac
     8221 gacaccgtgt tcatgcgcac cacccagggg ccgcggcggg tggatgtgat ctaccgccgc
     8281 ctcgacgacg acttcctcga ccccgaggtc ttccgcgccg actcgatgct cggtgtgccg
     8341 ggcctgatgt cggcctaccg cgccggccgg gtgacgctgg ccaacgcggt cggcacgggg
     8401 gtggcggacg acaagtcgat ctacccctac gtgcccgaga tggtgcgctt ctacctcggc
     8461 gaggagccga tcctcaacaa cgtcccgacc tggatgctgc gcgaacccga cgacctcgcc
     8521 tacacgctcg cccacctgcc cgagctggtg gtcaaggagg tccatggcgc cggcggctac
     8581 ggcatgctgg tggggccggc ggccaccaag gccgagatcg aggagttccg ccagcgcatc
     8641 ctcgccgcgc cggagaagta catcgcccag ccgaccttgt ccttgtccac ctgcccgacc
     8701 ttcgtcgacg ccggcatcgc gccgcgccac atcgacctgc gtcccttcgt gctctcgggc
     8761 ggacgcgaga tccgcatggt gccgggcggc ctcacccgcg tcgcgctcaa ggccggctcg
     8821 ctggtggtca actcctcgca gggcgggggc accaaggaca cctgggtggt gggctgagcc
     8881 gcccgccgcc gcgcaattgc caaggagaac ccccatgctg agccgaaccg cagaccacct
     8941 gtactggatg gcgcgctaca ccgagcgcgc cgagaacacc gtgcgcatgc tcgacgtgtc
     9001 ctaccgcatg tcgctgctgc ccggggccaa cggcggcgac accgagagct ggtccgccat
     9061 cctcgacatc agcgaactgt cggaggcctt cgcccgcacc ggcaggccgc tctccgcctg
     9121 cgacgtgatc gagttcgtcg tgctcgaccg cgacaacccc tcgagcgtgt ggaactgcct
     9181 gcgcgccgcg cgcgagaatg cgcacgcggt gcgcggcacg ctgaccgccg agatgtggga
     9241 gaccaccaac gcgacctggc tcgagatccg cgagatgacg ccgcagaagc tgcgcgccac
     9301 cgacctcggc gagttcttcg agtgggtgaa gttccgctcc cacctgctgc gcggggtgac
     9361 cttcggcacc atgctgcacg acgaggcctt caagttcgtg cgcctgggca ccttcctcga
     9421 gcgcgccgac aacaccgcgc gcctgctcga cgtgaagtac cacagcctgc tgcccaaggc
     9481 cgagggactc accagctcga ccgacttcta ccagtggggg gcgctgctgc gctcggtgtc
     9541 ggccttcgag acctaccgcc acgtgtatcg cgacgtgatc acaccctaca aggtggccga
     9601 gctgctgatc ctcaacgccg acatgccgcg ctcgctgcac gcctgcctgg acgagatcaa
     9661 cggcatcctc ggcgagatcg ccagcatcca ctcggccgag gccgcacgcc aggccggcga
     9721 gctgcacgcg acgctgcact acgggcgcat ggacgacatc ttcgatgccg gcctgcatcc
     9781 ctggctggag cgcttcctgg tgcgcatcaa ccgcctcggc gacagcatcg gcgagaactt
     9841 tctggttccg gtcgcggcat gaccggtacg cggcgtcttc cggcccggac ccggggccgg
     9901 gccaatgaac cgacgggatc gaacccatga cctactgcgt cgccatgtgc ctcgacgagg
     9961 ggctggtctg cctgtccgac tcgcgcacca acgcgggcgt ggaccacatc aacaccttcc
    10021 gcaagatgac cgtgttcgag cgtgccggcg accgcgtgct ggtgctgatg agcgccggca
    10081 acctcgccat cacccaggcg gtgatcagcc tgctgcgcga gcacgccgcg gtgcaggacc
    10141 gcgacagcct gtggaacgcg ccgaccctgt tcgagtgtgc ccgcctggtc ggcgagacca
    10201 tccgcggcgt gcatcgccag gacgccgagg cgctcaagca gttcggcgtg gagttcaacg
    10261 ccagcttcat cctcgctggg cagatcggcg acgagtcgcc gcgcctgttc aacatctact
    10321 cggccggcaa cttcatcgag gcctcgatcg acacgccgta cttccagatc ggcgagtcga
    10381 aatacggcaa gccgatcatc gaccgcatcg tcaccagcaa gacctcgctc gacgaggccg
    10441 ccaagtgcgc gctgatctcg atggactcga ccatcc
//
LOCUS       DAOQBS010000111        10210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_5853_length_10210_cov_16.390251, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000111 DAOQBS010000000
VERSION     DAOQBS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5853_length_10210_cov_16.390251"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2072)
                     /gene="metG"
                     /locus_tag="PKN25_11805"
     CDS             complement(<1..2072)
                     /gene="metG"
                     /locus_tag="PKN25_11805"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNV91626.1"
                     /translation="MSRKLLVTNALPYANGDIHLGHLVGYIQADIWVRYQRMRGHSVH
                     YVCADDTHGTPVMLRAEKEGLTPEQLIERVHGEHLRDFTAFGVAFDNYHSTHSAENRF
                     YAEDIYARLKAGGLIDTRSIEQYYDPVKEMFLPDRFIKGECPKCGAPDQYGDNCEACG
                     AAYAPTELKNPYSAVSGAKPVLKTSEHYFFRLSDERAVAFLREWTRGTNAAGERRLQM
                     EAANKMKEWLGENATEEGENTLSDWDISRDAPYFGFEIPGAPGKYFYVWLDAPIGYLA
                     SFRNLAAKRAAAGETIAVEDYTDAARAAAAGTEMVHFIGKDILYFHALFWPAMLKFAG
                     YATPSRLCVNGFLTVDGAKMSKSRGTFITAHSYIAQKLDPEWLRYYFAGKSNGTMEDV
                     DLNLDDMIAKVNSELVGKFVNIASRCAGFIGKRFDGRLGEADAAACAEFAAAWSEGRV
                     ATAFEERDYSRALREVMRLADLANQYVNDHKPWELARQEGQEARLHTVCSTALTMFRD
                     LTRYLKPVLPALAAQVETFLAIPAMDWQGDWAALPAGHAIQAYSHLMTRVERKQIDAL
                     LEANRESLAPATAVPAKTDKPAKDTEAASSQQRHAEKQQHSAQAAETASPHITIDDFT
                     KVELRIARIVDAQHVEGADKLIRLALDIGEADESGNPKPRQVFAGIKSAYDPATLIGR
                     LTVMVANLA"
     gene            complement(2150..2752)
                     /locus_tag="PKN25_11810"
     CDS             complement(2150..2752)
                     /locus_tag="PKN25_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV91627.1"
                     /translation="MHMTLRNSRRRPGFPTALVSATLLAGLLAACASPGGNGTATDAI
                     HRHALVRTAPPEPSWAALREQLAKATTGLAGVEIGSAGDTGFRVQIPVADGFASGSPA
                     IRPTLGRALDALAPALAAEAGVAVRVVGHTDSQGSEMVNLRLSIARAEAVVAWLRERG
                     IALDRLAADGRGEADPLTSNATEEGRVRNRRIELILSRMP"
     gene            2915..4006
                     /gene="apbC"
                     /locus_tag="PKN25_11815"
     CDS             2915..4006
                     /gene="apbC"
                     /locus_tag="PKN25_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNV91628.1"
                     /translation="MSLDQQTVTEALKQVIDPNTGKDFVAGRSIRNLSVQGGEVRFDV
                     ELGYPAKSQHDPIRALLSEAVAKLPGVERVAVTVSSKVVAHAVQQGVKLLPGVRNIIA
                     VASGKGGVGKSTTAVNLALALSAEGARVGLLDADIYGPSQPQMLGIGDQRPVSEDGKT
                     MTPLQAFGLQAMSIGFLIDPDTPMVWRGPMATQALNQMLKDTAWDDLDYLVIDMPPGT
                     GDIQLTLSQSVPVTGAVIVTTPQDIALLDARKGVKMFEKVGVPILGVVENMSIHICSK
                     CGHEEHIFGQGGGEKMCADFKVPFLGALPLDIQIRTEADSGAPTVVADPDGRIASIYK
                     QIARKVAVRIAERSKDMTHKFPNIVFKSA"
     gene            4198..4764
                     /gene="dcd"
                     /locus_tag="PKN25_11820"
     CDS             4198..4764
                     /gene="dcd"
                     /locus_tag="PKN25_11820"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNV91629.1"
                     /translation="MSIKSDKWIRRMAEQARMIEPFAPELVRSNDTGRIVSYGTSSYG
                     YDVRVANEFKIFTNINSTIVDPKDFDARNFVDFTGDVCIIPPNSFALARTVEYFRIPR
                     SVLTVCLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYANEGVAQMLFF
                     ESDEVCETSYRDRGGKYLGQTGVTLPKI"
     gene            4914..7157
                     /locus_tag="PKN25_11825"
     CDS             4914..7157
                     /locus_tag="PKN25_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HNV91630.1"
                     /translation="MRFHFPIIIIDEDFRSENTSGLGIRALAKAIEEEGMEVLGVTSY
                     GDLTSFAQQQSRGSTFILSIDDEEFSSPEASAKAIADLRAFVVEIRFRNADIPIFLHG
                     ETRTTRHIPNDVLREMHGFIHMFEDTPEFIARYVVREAKNYLDSLAPPFFRALVHYAA
                     DSSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVDELGQLLDHTGPVAA
                     SERNAARIFNCDHLYFVTNGTSTSNKMVWHTTVAPGDIVVVDRNCHKSILHSIIMTGA
                     VPVFLTPTRNHYGIIGPIPLEEFSMENIRKKIEANPFAREAKNKKPRILTITQSTYDG
                     VVYNVEAIKELLDGEIDTLHFDEAWLPHAAFHDFYGDYHAIGADRPRCRESMVFATQS
                     THKLLAGLSQASQILVQDSQTRKLDRDIFNEAYLMHTSTSPQYAIIASCDVAAAMMEA
                     PGGTALVNESLSEAVEFRRAMRKVDAEFGDDEWWFQVWGPEYLAEEGIGERDDWTLKA
                     GERWHGFGNLAEGFNILDPIKATIITPGLNMDGDFAEHTGIPAAIVTKYLAEHGIIVE
                     KTGLYSFFIMFTIGITKGRWNTMVTELQQFKDDYDRNQPLWRVMPEFIAKHPRYERVG
                     LKDLCDQIHSFYKANDVARLTTEMYLSDMVPAMKPADAFAKMAHREIERVALDELEGR
                     VTAMLVTPYPPGIPLLIPGERFNATIVRYLKFARDFNARFPGFETDIHGLVKGEDGRY
                     HVDCVKG"
     gene            complement(7251..7745)
                     /locus_tag="PKN25_11830"
     CDS             complement(7251..7745)
                     /locus_tag="PKN25_11830"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNV91631.1"
                     /translation="MKKPEIVLIAAVARNGTIGRDNELPWRLKADLQHFRALTLGHPI
                     LMGRKTWQSLGRPLPGRRNLVVSRTPDYRAEGAECFADPAAAIAAAGEAQTLFVIGGA
                     QLYAQLLPLADRLELTEVKADVEGDAHFPAVDPARFVEERRETHHADADNAFDFDFVR
                     YCRR"
     gene            complement(7742..8536)
                     /locus_tag="PKN25_11835"
     CDS             complement(7742..8536)
                     /locus_tag="PKN25_11835"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNV91632.1"
                     /translation="MRQYLDLMRHVLEHGDPKTDRTGTGTLSVFGWQMRFRLQDGFPL
                     LTTKKLHTRSIIHELLWFLQGETNIRYLKENGVSIWDEWADADGELGPVYGKQWRRWE
                     TADGRSVDQIARVVDSIRRTPDSRRHLVSAWNPGEVDAMALPPCHALFQFYVAGGRLS
                     CQLYQRSADIFLGVPFNIASYALLTHMVAQVCELEAGDFVWTGGDCHLYSNHLRQARE
                     QLERAPRPLPTLRLNPAVRDLFGFRFEDIALEGYDPHPHIKAPVAV"
     gene            8737..10137
                     /locus_tag="PKN25_11840"
     CDS             8737..10137
                     /locus_tag="PKN25_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91633.1"
                     /translation="MDQPRTLDYTPAGVLAGGGNPVAGLEAATATLRALYKPGSQKTE
                     VAGLHVVSSFDDALREAVERSAPVGVRALAGAAPERDHAWWVRWLAGVRDAWMAVCDG
                     YWCLSPSNSVAASEEIRLRLASSVVEALKWSACDRFGPEEVLWGRIGQLFSSSPQARD
                     SVVGGDLHSIGREYLRAVAHYSINLDQVELEMGVALGHVVDICLPFLSLDRRAADVPQ
                     YVVIPEEGTIPVRQMGKHRDGEWRFAPWAAGELLTEFSRQLARSIVPAPFGRLPLEHA
                     RAAVEYLRMQWSHTPPVRTRRRYAQEASADIARGLHACAAVIGGQPLPVPRNWRVVDF
                     SRSGLQIVANSDPTRAWPDIGELLGIHFVDGEGWQLGIVRRLRMWAAHAGLGIELLAR
                     SPTLGVIDDGRASVDGILCDLPSKGEALRVLMPANAPALAEPVFVKTAGAVHKLRSLG
                     VLRREAGYQLQVFQVL"
BASE COUNT         1624 a         3313 c         3541 g         1732 t
ORIGIN      
        1 gcgaggttgg cgaccatcac cgtcaggcgc ccgatgagcg tcgccgggtc gtaggccgac
       61 ttgatgcccg cgaagacctg gcgcggtttc gggttgccgg actcgtcggc ctcgccgatg
      121 tcgagcgcaa gccggatcag tttgtcggcg ccttcgacgt gctgggcatc gacgatcctc
      181 gcgatgcgca gctcgacctt ggtgaaatcg tcgatcgtga tatgcggcga ggcggtctcc
      241 gcagcctggg ccgaatgctg ctgtttctcg gcgtggcgct gctgcgagga cgccgcctcg
      301 gtgtccttgg caggcttgtc tgtcttcgcg ggcaccgccg tcgccggggc gagcgactcg
      361 cggttggcct cgagcagggc gtcgatctgc ttgcgctcga cgcgggtcat caggtggctg
      421 taggcctgga tggcatggcc ggcaggcagc gccgcccagt cgccctgcca gtccatggcc
      481 gggatggcga ggaaggtttc gacctgcgcg gcgagcgccg gcagcaccgg cttcaggtag
      541 cgggtgaggt cgcggaacat ggtcagcgcg gtgctgcaca ccgtgtgcag gcgggcttcc
      601 tggccttcct gcctggcgag ttcccagggc ttgtggtcgt tcacgtactg gttggcgagg
      661 tcggccaggc gcatgacctc gcgcagcgcg cggctgtagt cgcgctcctc gaacgcggtg
      721 gcgactcggc cctcgctcca ggcggcggcg aactcggcac aggcggcggc gtcggcctca
      781 cccaggcggc cgtcgaagcg cttgccgatg aagccggcgc agcggctggc gatgttcacg
      841 aacttgccca cgagctcgga attgaccttg gcgatcatgt cgtcgaggtt gaggtcgacg
      901 tcctccatgg tgccgttcga cttgccggcg aagtagtagc gcagccactc ggggtcgagc
      961 ttctgcgcga tgtaggagtg cgcggtgatg aaggtgccgc ggctcttgct catcttggcg
     1021 ccgtcgacgg tgaggaagcc gttcacgcac agccggctgg gcgtggcgta gccggcgaac
     1081 ttcagcatcg cgggccagaa cagggcgtgg aagtagagga tgtccttgcc gatgaagtgc
     1141 accatctcgg tgccggccgc ggcggcgcgg gcggcgtcgg tgtagtcctc gacggcgatc
     1201 gtctcgcccg cggcggcgcg cttggcagcg aggttgcgga agctggcgag gtagccgatc
     1261 ggtgcgtcca gccacacata gaagtacttg cccggcgcac ccggaatctc gaagccgaag
     1321 tagggtgcgt cgcgcgagat gtcccagtcg gagagcgtgt tctcgccttc ctcagttgcg
     1381 ttttctccga gccactcctt catcttgttg gcggcttcca tctgcaggcg gcgctcgccg
     1441 gcggcgttgg ttccgcgcgt ccattcgcgc aggaaggcga ccgcgcgttc gtccgacaga
     1501 cggaagaagt agtgctccga ggtcttcagc accggcttgg cgccggacac cgcggaatag
     1561 gggttcttga gctcggtggg ggcgtaggcc gcgccgcagg cctcgcagtt gtcgccgtac
     1621 tggtcggggg cgccgcactt cgggcactcg cccttgatga agcggtcggg gaggaacatc
     1681 tccttgaccg ggtcgtagta ctgctcgatc gagcgtgtgt cgatcaggcc gcccgccttc
     1741 agtctggcgt agatgtcctc ggcatagaaa cggttctcgg cgctgtgcgt cgagtggtaa
     1801 ttgtcgaatg cgacgccgaa ggcggtgaag tcgcgcaggt gctcgccgtg cacgcgctcg
     1861 atgagctgct cgggcgtgag cccttccttt tcggcgcgca gcatgaccgg cgtgccgtgg
     1921 gtgtcgtccg cgcacacgta gtgcaccgag tggccgcgca tgcgctggta gcgcacccag
     1981 atgtcggcct ggatgtagcc cacgaggtgg ccgaggtgga tgtcgccgtt ggcgtagggc
     2041 agggcgttgg tgacgaggag cttgcgggac atgatgggtg cgggaaccgg tgccggataa
     2101 agtcgagagt ttagcaaagc ggcacgcgat gccgcgccgt ggcgcgcgct cagggcatgc
     2161 gcgagaggat cagctcgatg cggcggttgc gcacgcggcc ttcctcggtg gcgttggacg
     2221 tgagcggatc ggcctcgccg cgaccgtcgg cggcgaggcg gtcgagggcg atgccgcgct
     2281 cgcgcagcca cgccaccact gcctccgcgc gcgcgatcga caggcgcagg ttgaccatct
     2341 cgctgccttg gctgtcggtg tggccgacca cccgcaccgc cactccggcc tcggccgcga
     2401 gcgcgggcgc gagcgcgtcg agcgcgcgtc cgagcgtcgg acggattgcc ggactgccgc
     2461 tggcgaagcc gtcggcgacc gggatctgca cgcggaagcc cgtgtcgccg gcgctgccga
     2521 tctcgactcc cgcgaggccg gtggtggcct tggcgagctg ctcgcgaagt gcggcccagg
     2581 acggttcggg cggtgcggtc cgcaccagcg catggcggtg gatcgcgtcg gtggccgtgc
     2641 cattgccgcc agggctggcg caggcggcga ggaggccggc gagcagggtg gcggacacga
     2701 gggccgtcgg gaagccgggt ctgcggcggg aattgcgcaa agtcatgtgc atgtggcgcc
     2761 tgcatgcggc gcgcgggaaa ttcgtcgaac ggggtcgggt gcgggggcgg gcgagaaacc
     2821 ctgcgcagaa cgcggatgag tccggcgctg cgctccggta tgattctgcc gcttccaccc
     2881 ggcggcgggt gcaccgattt tcgaggaaaa cgcgatgagt cttgatcagc aaaccgtaac
     2941 cgaagcgctc aagcaggtga tcgatcccaa caccggcaag gatttcgtgg cggggcgcag
     3001 catccgcaac ctgtcggtgc agggcggcga ggtccgcttc gacgtcgagc tcggctaccc
     3061 ggcgaagagc cagcacgatc cgatccgcgc gctgctgagc gaggccgtgg ccaagctgcc
     3121 gggcgtggag cgcgtcgccg tgacggtgtc gagcaaggtg gtcgcgcacg cggtgcagca
     3181 gggcgtcaag ctgctgccgg gcgtgcgcaa catcatcgcg gtggcctcgg gcaagggcgg
     3241 cgtgggcaag agcacgaccg cggtgaacct cgcgctcgcg ttgtcggccg aaggtgcccg
     3301 cgtcggcctg ctcgacgccg acatctacgg tccctcgcag ccacagatgc tgggcatcgg
     3361 cgaccagcgc ccggtgtcgg aggacggcaa gaccatgacc ccgctgcagg ccttcgggct
     3421 gcaggcgatg tcgatcggct tcctgatcga ccccgatacg ccgatggtgt ggcgcggccc
     3481 gatggccacg caggcgctca accagatgct caaggacacc gcctgggacg acctcgacta
     3541 cctcgtcatc gacatgcccc cgggcaccgg cgacatccag ctcaccctgt cgcagagcgt
     3601 gccggtgacc ggcgcggtga tcgtcaccac gccgcaggac atcgcgctgc ttgacgcgcg
     3661 caagggcgtg aagatgttcg agaaggtggg cgtgcccatc ctcggcgtgg tcgagaacat
     3721 gagcatccac atctgctcga agtgcggcca cgaggagcac atcttcggcc agggtggcgg
     3781 cgagaagatg tgcgcggact tcaaggtgcc cttcctcggt gcgctgccgc tcgacatcca
     3841 gatccgcacc gaggccgaca gcggcgcgcc caccgtggtc gccgatccgg atggccgcat
     3901 cgcctcgatc tacaagcaga tcgcgcgcaa ggtggcggtg cgcatcgccg agcgctcgaa
     3961 ggacatgacg cacaagttcc ccaacatcgt gttcaagtcc gcctgattgc gcaggccgcg
     4021 cccgtccgta gcggcgggcg cgcacgtcga gcgtgcccgc cgtgcgccaa cttcgcgcga
     4081 tccggccgcg attcgttttc cctccgcctt tcgtccgccg cttgcctgcc ttacactacg
     4141 cggcttttca cggacggcct ccgccgcccg caccgcaacc cgccggaaga cgcgcccatg
     4201 tccatcaagt ccgacaagtg gatccgccgc atggccgaac aggcccgcat gatcgagccc
     4261 ttcgcgccgg aactggtgcg cagcaacgac accggccgca tcgtgtccta cggcacctcg
     4321 agctatggct acgacgtgcg cgtggcgaac gaattcaaga tcttcaccaa catcaactcg
     4381 accatcgtcg accccaagga cttcgatgcg cgcaacttcg tcgacttcac cggcgacgtg
     4441 tgcatcatcc cgccgaactc ctttgcactc gcgcgcacgg tcgagtactt ccgcattccg
     4501 cgcagcgtgc tgaccgtgtg cctgggcaaa agcacctacg cgcgctgcgg catcatcgtg
     4561 aacgtcaccc cgctcgagcc cgagtgggaa ggccacgtga ccctcgagtt ctccaacacc
     4621 acgccgctgc cggcgaagat ctacgccaac gagggcgtgg cgcagatgct gttcttcgag
     4681 tcggacgagg tgtgcgagac ctcgtacagg gatcgcggcg gcaagtacct cgggcagacc
     4741 ggcgtcaccc tgcccaagat ctgagcgcct tcgcttcgcc gaccttcctc cacggggccg
     4801 cctgacggcc ccttgtgctt tttcgaacca atttcggtaa cgcgcgggca atcttcgccg
     4861 gagtacactg ccggctttac ccggcccgca cggacccgct ggagacgacc aggatgagat
     4921 tccacttccc catcatcatc atcgacgagg acttccgctc ggagaacacc tcgggactgg
     4981 ggatccgcgc gctcgccaag gcgatcgagg aagagggcat ggaagtgctc ggcgtcacca
     5041 gctacggcga cctgacgtcc ttcgcgcagc agcagagccg tggctcgacc ttcatcctgt
     5101 cgatcgacga cgaggagttc tcctcgcccg aggcctcggc caaggcgatc gccgacctgc
     5161 gcgcgtttgt cgtcgagatc cgcttccgca acgccgacat cccgatcttc ctgcacggcg
     5221 agacgcgcac cacgcgccac atccccaacg acgtgctgcg cgagatgcac ggcttcatcc
     5281 acatgttcga ggacacgccg gagttcatcg cgcgctacgt ggtgcgcgag gcgaagaact
     5341 atctcgattc gctggcgccg cccttcttcc gcgcgctggt gcactacgcg gccgacagct
     5401 cgtactcgtg gcactgcccg gggcactcgg gtggcgttgc ttttctgaaa agcccggtgg
     5461 ggcagatgtt ccaccagttc ttcggcgaga acatgctgcg cgcggacgtg tgcaatgcgg
     5521 tcgacgaact cggccagctg ctcgaccaca ccgggccggt ggcggcctcg gagcgcaacg
     5581 cggcgcgcat cttcaactgc gaccacctgt acttcgtcac caacggcacg tcgacgtcga
     5641 acaagatggt ctggcacacc acggtggcgc ccggcgacat cgtggtcgtc gaccgcaact
     5701 gccacaagtc catcctccac tcgatcatca tgaccggcgc ggtgccggtg ttcctgacgc
     5761 cgacgcgcaa ccactacggc atcatcggcc cgatcccgct ggaggagttc tcaatggaga
     5821 acatccgcaa gaagatcgag gccaacccct tcgcgcgcga ggcgaagaac aagaagccgc
     5881 gcatcctcac catcacccag tcgacctacg acggcgtggt gtacaacgtc gaggccatca
     5941 aggagctgct cgacggcgag atcgacaccc tgcacttcga tgaagcctgg ctgccgcacg
     6001 ccgccttcca cgacttctac ggcgactatc acgcgatcgg cgccgatcgt ccgcgctgcc
     6061 gcgagtcgat ggtgttcgcc acccagtcca cccacaagct gctcgccgga ctcagccagg
     6121 cctcgcagat cctggtgcag gactcgcaga cccgcaagct cgaccgcgac atcttcaacg
     6181 aggcctacct catgcatacc tcgacctcgc cgcagtacgc gatcatcgcg tcctgtgacg
     6241 tggcggcggc gatgatggag gcgccgggcg gcaccgcgct ggtcaatgaa tcgctctccg
     6301 aggcggtgga gttccgtcgc gcgatgcgca aggtggacgc cgaattcggc gacgacgaat
     6361 ggtggttcca ggtctggggg ccggagtacc tggccgagga gggcatcggc gagcgcgacg
     6421 actggacgct gaaggccggc gagcgctggc atggcttcgg caacctggcc gagggcttca
     6481 acatcctcga cccgatcaag gcaacgatca tcaccccggg cctgaacatg gacggcgact
     6541 tcgccgaaca caccggcatc ccggcggcga tcgtcaccaa gtacctcgcg gagcacggca
     6601 tcatcgtcga gaagaccggc ctgtactcct tcttcatcat gttcaccatc ggcatcacca
     6661 agggccgctg gaatacgatg gtgaccgagc tgcagcagtt caaggacgac tacgaccgca
     6721 accagccgct gtggcgcgtg atgcccgaat tcatcgccaa gcatccgcgc tacgagcgcg
     6781 tcgggctcaa ggatctgtgc gaccagatcc acagcttcta caaggccaac gacgtcgccc
     6841 gactgaccac cgagatgtat ctgtcggaca tggtgccggc gatgaagccc gccgacgcct
     6901 tcgccaagat ggcgcaccgc gagatcgagc gcgtggcgct cgacgagctc gagggccgcg
     6961 tcaccgcgat gctggtcacg ccctatccgc cgggcatccc gctgctgatc ccgggcgagc
     7021 gcttcaacgc gacgatcgtg cgctacctca agttcgcgcg cgacttcaac gcccgcttcc
     7081 ccggcttcga gaccgacatc cacggcctgg tcaagggcga ggacgggcgc taccacgtgg
     7141 attgcgtgaa gggctgagcc gctcccgccg cagggggtgt cgaatgacga gggtcgcgcg
     7201 atgcggccct tgtcgtttct gcggcagggc agtcggcgcg gccgtcgggc tcagcgccgg
     7261 cagtagcgca cgaaatcgaa gtcgaaggcg ttgtcggcgt cggcgtggtg tgtctcgcga
     7321 cgctcctcga cgaaccgcgc cggatcgacg gcggggaagt gcgcatcgcc ctcgacgtcg
     7381 gctttcacct cggtgagctc gaggcggtcg gcgaggggaa gcagctgggc gtaaagttgg
     7441 gcgccgccga tcacgaacag tgtctgggcc tcgccggccg cggcgatcgc ggccgcgggg
     7501 tcggcgaagc attcggcgcc ttcggcacgg taatccggcg tacggctcac cacgaggttg
     7561 cgccgtcctg gcagcgggcg gccgagcgat tgccaagtct tgcgccccat caggatcggg
     7621 tgccccaggg tgagggcacg gaaatgctgc aggtcggcct tcagccgcca gggcagctcg
     7681 ttatcgcggc cgatcgtgcc gttgcgcgcg accgcggcga tgaggacgat ctcgggcttc
     7741 ttcataccgc caccggggcc ttgatgtgcg gatgcgggtc gtagccttcg agcgcgatgt
     7801 cctcgaagcg gaagccgaag aggtcgcgca ctgcggggtt caggcgcagc gtcggcaggg
     7861 ggcgcggggc gcgctcgagc tgctcccgcg cctgcctcag gtggttgctg tagaggtggc
     7921 agtcgccacc ggtccacacg aaatcgcccg cttcgagctc gcacacctgc gccaccatgt
     7981 gggtgagcag ggcataggag gcgatgttga agggcacgcc gaggaagatg tccgcgctgc
     8041 gctggtagag ctggcaggac aggcgaccgc cggcaacgta gaactggaaa agcgcgtggc
     8101 agggcggcag ggccatggcg tcgacctcac ccggattcca cgccgagacg agatggcggc
     8161 gcgaatccgg ggtgcgccgg atgctgtcga cgacgcgtgc gatctggtcg acgctgcgcc
     8221 cgtctgcggt ctcccagcgc cgccactgct tgccgtacac cgggccaagc tcgccgtcgg
     8281 cgtccgccca ttcgtcccag atcgacacgc cgttttcctt gaggtagcgg atgttggtct
     8341 cgccctgcag gaaccacagc aactcgtgga tgatcgagcg cgtgtgcagc ttcttggtgg
     8401 tcaggagcgg aaaaccgtcc tgcaggcgga agcgcatctg ccagccgaac accgacaggg
     8461 ttccggtgcc ggtgcggtcg gtcttggggt cgccgtgctc gagcacatgg cgcatcaggt
     8521 cgaggtactg tcgcatcgtc gctcccggaa aaggcgagat tctactgtgc agggcgaagg
     8581 atctgtgatg gcggtggcaa tacgatcgct tcgtcccggc gtgtatgcgg tggacatcgc
     8641 cgtggacgct ggttcagaat gtgacgaaat ctttcgtgcg acgcctgttt tccctgggga
     8701 atacaggcag ggcgcggttt gtgcggagaa agccctttgg atcagcccag gacgctcgac
     8761 tacacgcctg ccggtgtgct cgctggtgga ggcaacccgg tcgcgggcct ggaggcggcg
     8821 actgcgacgc tgcgggcatt gtacaaaccg ggaagccaga agacggaggt cgcgggcttg
     8881 catgtggtgt ccagcttcga cgacgccttg cgtgaagcag tcgagcgctc ggcgccggtc
     8941 ggggtgcgcg ctctggcagg cgccgcgccc gagcgtgacc acgcgtggtg ggtgcgttgg
     9001 ctcgcagggg tccgggatgc ctggatggca gtgtgcgatg gctattggtg tctctccccg
     9061 tcgaactcgg tggccgcctc cgaagagatc cggttgcgtc tcgcgagcag cgtggtcgag
     9121 gcgctgaagt ggtccgcctg cgatcgcttc ggccccgagg aggtgctctg ggggcgcatc
     9181 ggccagttgt tctcttcctc gccgcaggcc cgcgacagcg tcgtcggcgg agacttgcac
     9241 agcatcgggc gcgaatacct gcgcgcggtc gcccactaca gcatcaacct cgatcaggtc
     9301 gagctcgaga tgggcgtcgc gctcgggcac gtggtggata tttgccttcc gtttctctcc
     9361 ctcgatcggc gcgcggcgga tgttccgcag tatgtggtca tccccgagga gggaaccatc
     9421 ccggtgcgcc agatgggcaa gcaccgggat ggcgagtggc gtttcgcccc gtgggcggcg
     9481 ggggagttgc tgaccgagtt ctcgaggcag ctcgcgcgct cgatcgtgcc cgctccgttc
     9541 gggcgcctgc cactcgagca tgcccgtgca gccgtcgaat acctgcgcat gcagtggtcg
     9601 catacccctc cggtgcgcac acgacgccgt tacgcccagg aggcgagcgc agatatcgcg
     9661 cggggattgc acgcctgtgc cgcggtgatc ggcgggcagc ccttgccggt gccgcgaaac
     9721 tggagggtgg tcgatttcag tcgcagcggc ctgcagattg tcgccaacag cgacccgacg
     9781 cgcgcgtggc cagacatcgg cgagctcctc ggcatccatt tcgtggacgg cgaaggctgg
     9841 cagcttggca tcgtgcgccg gttgcgcatg tgggctgcgc acgcgggtct gggcatcgag
     9901 ttgctggctc gcagtccgac gctcggcgtg atcgatgacg gtcgcgccag cgtggacggc
     9961 atcctgtgcg atctcccgag caagggcgag gccttgcgcg tcttgatgcc cgccaatgcg
    10021 cctgcgctcg ccgagccggt cttcgtcaag accgcggggg cggtccataa actacgctcg
    10081 ctcggtgttc tccggcgcga agccggttat caactgcagg tgttccaggt tctgtaagtc
    10141 cttaggcgct ccttggccga tccgatcggc cgttcgcggt tgtcggatgc aaggggggcg
    10201 gggatttctt
//
LOCUS       DAOQBS010000112         9856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_6205_length_9856_cov_16.492297, whole genome shotgun sequence.
ACCESSION   DAOQBS010000112 DAOQBS010000000
VERSION     DAOQBS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6205_length_9856_cov_16.492297"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="PKN25_11845"
     CDS             <1..1380
                     /locus_tag="PKN25_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNV91634.1"
                     /translation="GVLLFAMGALRLGNMIRFIPLSVVIGFTNGIAVVIFISQIKDFL
                     GLQIADLPGEFFAKMGALAAALPTLHLPTVAVALVSLAILVAWNRQAVKTAWMRRLPG
                     PLAVLIILTAINALIALPVDTIGSRFGGIPQEIPPFGLPELSLSTLGKLIAPAITIAL
                     LGAIESLLSARVADSQIDDRHDPNQELMAQGIANVAAPLFGGFAATGAIARTATNIRT
                     GGRTPIAGMIHAGVLLAIVLALAPLASHIPLATLSAIVVIVSINMGEWHAFAPKELAR
                     YSLNYRTILLGTFFITVVFDLTLAVEIGMALASLFFIYRMSDLTRIDRVPLEDFYGVE
                     ALSHPDGTPRIVAYKLKGSLFFGAANKLENLLLGLPGHPEVVVLDLDKVINIDTTGLD
                     ILQTLHRNLQKRGAHLVLCGLNSQPGSIVFRSGFSDKLGDDNIAANLTEALIRAQRLS
                     GREPEIAYA"
     gene            1415..1741
                     /locus_tag="PKN25_11850"
     CDS             1415..1741
                     /locus_tag="PKN25_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNV91635.1"
                     /translation="MPDGKSPLPAASAPAADAIARRLRIHGRVQGVWYRGSATAEAAR
                     LGLSGWVRNRSDGTVEALAIGPLVKVETFVAWAHRGPENARVSRVEVFEAELERLTGF
                     EQRPTV"
     gene            complement(1961..5485)
                     /gene="dnaE"
                     /locus_tag="PKN25_11855"
     CDS             complement(1961..5485)
                     /gene="dnaE"
                     /locus_tag="PKN25_11855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNV91636.1"
                     /translation="MNPAAPSTAEAPIVPSAPRFVHLRLHSEYSITDGIVQLDQAIAA
                     AAADGMPALGVSDLANLFGMVKFYKGARGKGIKPIVGVDAWIRNEAERDKPQRVLLIC
                     KNRAGYGQLCELLTRAYLENKHRGRAEMRREWFENGAASALLCLSGAMSGDIGAAIAA
                     GNLALAEQLAADWARLFPDAFYIEIQRAGHPGTESYIRHAVELAGRLGLPVVATHPVQ
                     FLKREDFKAHEARVCIAQGYVLADKRRPRDFTEEQYLKSQAEMCELFADLPEALENAV
                     EIARRCSLTVQLGKNFLPLFPTPEGMTLDDFLVAEAKKGLEERLAQLYPHPEERERQR
                     PRYEQRLKFETDTIIQMGFPGYFLIVADFIQWGKNNGVPVGPGRGSGAGSLVAYSLKI
                     TDIDPLEYALLFERFLNPERVSMPDFDIDFCQDNRYRVIEYVRERYGKDAVSQIATFG
                     TMASKAVVRDVGRVLDLPYGLCDRLSKLIPIEGAKPVSLNKAYEMEPQIGEMMADGND
                     GESVRDLWSLAQPLEGLSRNVGMHAGGVLIAPGKLTDFCPLYIADGDDATPVSQFDKD
                     DVEAVGLVKFDFLGLRNLTIIELALEYVARLEGSRPDLMSLGFEDPAAYQILKDANTT
                     AIFQVESDGMKKLLKKLAPDRFEDIIAVLALYRPGPLGSGMVDDFILRKKGQQEIDYF
                     HPDLKACLEPTYGVIVYQEQVMQISQIIGGYTLGGADMLRRAMGKKKADEMAKHRATI
                     AEGAKQKGYDPALAEQLFDLMTKFAEYGFNKSHTAAYAVVTYHTAWLKAHHCAAFMAA
                     TMSSDLDNTDTVKIFYEDTLANGIKVLPPDVNASDYRFVPVDRKIIRYGLGAVKGVGE
                     PAVRAILAAHEKGGDFRDLFDFCERVDRRMVNRRVIEALIRAGAMDTLPGHKGLDRAQ
                     LMATVALAMEAAEQAAANAMQGGLFDLMPEAAGAAPEFAKVRAWTERERLKEEKLAIG
                     FFLSGHPFNAFKPEVRRFVRRTLAQLEPSRDITMLAGVVMEQRTKVGNRGKMAFVLLD
                     DGTEPREVTVYSEVLEASRGKIVTDEVLVVEAKVSNDDFSGGLRINADRLLTLGEARS
                     RFARALSLRIDGELAASGGAAGAAGKLQTLLEPFREGGCPIRVRYRNAAAEAELPLGD
                     GWRVRLDDALLESLREWLPGEAVEVVYP"
     gene            complement(5529..6503)
                     /locus_tag="PKN25_11860"
     CDS             complement(5529..6503)
                     /locus_tag="PKN25_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HNV91637.1"
                     /translation="MTHAIRIHRTGGPEVLQWEAVELPPPATGEVRLRHHAVGLNFID
                     TYHRSGLYPLTLPSGLGMEGAGVVEAVGDGVAELKVGDRVAYTSGPLDAYAEARNIEA
                     RHLVPLPDDISFEQGAAMMLQGITAQYLLHATYPVKAGETILVHAAAGGVGSILVQWA
                     KQLGATVIGTVGNDDKAQRARALGCDHVIVYSRENFPERVRALTGGEGVAVVYDSVGR
                     DTFLDSVACLRRRGTMVSYGNATGPVAAFDCALLHKAGSVYVTRPGLPDYIASRAELV
                     ERAAALFEVVRSGQVRIEILQRYALKDAAQAHRDLEARKTTGSTVLLP"
     gene            complement(6571..7794)
                     /locus_tag="PKN25_11865"
     CDS             complement(6571..7794)
                     /locus_tag="PKN25_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HNV91638.1"
                     /translation="MPKTSSPRPLLLAGALALAAPAAFAADWLLATSSPQVVPGQRFE
                     VVVIGEGRAADWPARLPASIELSGGGPRIAVELVAIGRPAAGGGQRRYFARWPMEVTG
                     VAALALVGRPSARVLLDAGAAAGVATAATASTAIPPVAAAGTTAVAPAAAVAPMAPVA
                     AVPSDAAPVEPSGFGFHEPMYFLIGGRDPVSARFQFSFRYRIFDEQGVVAETIPVASG
                     LYFGFTQTSLWDLQSESKPFRDSSFRPSLFYRFALDDPQKRGSLALSGGYEHESNGKE
                     DMPSRSIDTLFVRADARLRLDESGSYLGVVPKAWTYLDREDNPDIARYRGHAELGLRL
                     GRDDGWLFSTLIRRGTEGKMGTQYDLSYPIRRSVFSGVGAFVHLQAFKGYGETLLEYD
                     ENKEAQYRIGVSLVR"
     gene            complement(7929..>9856)
                     /locus_tag="PKN25_11870"
     CDS             complement(7929..>9856)
                     /locus_tag="PKN25_11870"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNV91639.1"
                     /translation="AALEPTLGGINLEDIKAPECFYIEKKLRERMKIPVFHDDQHGTA
                     IISAAGLVNGLKVVGKNIADVKLVCSGAGAAAIACLDMMVSVGLRRENIYVCDSKGVI
                     YEGREPNMEPTKARYAQKTEGRALGDVIAGADVFLGLSTAGVLKPEMVAKMAAKPLIF
                     ALANPNPEILPADAKAVRPDCIVATGRSDFPNQVNNVLCFPFIFRGALDVGATTINEE
                     MKLACVKAIAELAEAEASDIVASAYGGQELSFGSEYIIPKPFDPRLIVKIAPAVAKAA
                     MDSGVATKPIADLEAYASSLNAFVYQSGIVMKPVFSAAKAVPLEQKRVIYAEGEDERV
                     LHAVRVVLDEGLARPILIGRPEVIEMRIKKIGLNLKPERDFEIVNPESDPRYRELWTE
                     YYKLMKRDGVTPDLAKAKIRRDTTLIGVMMLRRGDADALVCGTFGSYDYHLKQVADVI
                     GLAPGAKQFAAMNLLLLTKRMLAITDTYVNENPSAEEVAEIARMASDELRRFGIEPSV
                     ALLSHSNFGSSNSASARKMRRACEILRETSPDLNVDGEMHGDAALSEEIRDKLHPDSG
                     LKGEANLLVMPNLDAANISFNLMKVANGDGVSVGPILLGAAKPVHIVTPSATVRRLVN
                     MTALAVVDAKESRNAGA"
BASE COUNT         1499 a         3436 c         3363 g         1558 t
ORIGIN      
        1 ggcgtgctgc tgttcgccat gggcgcgctg cgcctgggca acatgatccg cttcatcccg
       61 ctgtcggtcg tgatcggctt caccaacggc atcgcggtgg tgatcttcat ctcgcagatc
      121 aaggacttcc tcggcctgca gatcgccgac ctgccgggcg agttcttcgc caagatgggg
      181 gcgctcgccg cggcgctgcc cacgctgcac ctgcccacgg tggcggtggc gctcgtgtcg
      241 ctcgcgatcc tggtggcgtg gaaccgccag gccgtgaaga ccgcgtggat gcgccgcctg
      301 cccggcccgc tcgcggtact gatcatcctg accgcgatca acgccctgat cgcgctcccg
      361 gtcgacacca tcggcagccg cttcggcggc atcccgcagg aaattccccc cttcggcctg
      421 ccggaactga gcctgtccac gctcggcaag ctgatcgcgc cggcgatcac gatcgcgctg
      481 ctcggcgcga tcgagtcgct cctctcggcg cgcgtcgccg acagccagat cgacgaccgc
      541 cacgacccca accaggagct gatggcgcag ggcatcgcca acgtcgccgc gccgctcttc
      601 ggcggcttcg ccgccaccgg cgcgatcgcg cgcaccgcga ccaacatccg caccggcgga
      661 cgcaccccga tcgccggcat gatccacgcc ggcgtgctgc tcgcgatcgt gctcgcgctc
      721 gccccgctcg ccagccacat cccgctcgcc acgctgtcgg cgatcgtggt catcgtgtcg
      781 atcaacatgg gagagtggca cgccttcgcg ccgaaggagc tcgcgcgcta ctcgctcaac
      841 taccgcacca tcctgctcgg caccttcttc atcaccgtgg tgttcgacct caccctggcg
      901 gtcgagatcg ggatggcgct cgcgagccta ttcttcatct accgcatgtc cgacctcacc
      961 cgcatcgacc gcgtgccgct ggaagacttc tatggcgtcg aagccttgtc ccaccccgac
     1021 ggcacgccgc gcatcgtcgc ctacaagctc aagggcagcc tcttcttcgg cgccgccaac
     1081 aagctcgaga acctgctgct cgggctgccc ggccaccccg aggtggtcgt gctggacctc
     1141 gacaaggtga tcaacatcga caccaccggc ctggacatcc tgcagaccct gcaccgcaac
     1201 ctgcagaagc gcggcgccca cctcgtgctg tgcggactca actcgcaacc cggctcgatc
     1261 gtgttccgct cgggcttcag cgacaagctc ggcgacgaca acatcgccgc caacctcacc
     1321 gaggcgctga tccgcgccca gcgcctgagc gggcgcgagc cggagatcgc ctatgcctga
     1381 gcggaaatcg cccacgcctg aacagcactc gagcatgccc gacgggaaat caccgctgcc
     1441 cgctgcaagc gcgccggcgg cggacgccat cgcccgccgc ctgcgcatcc acggcagggt
     1501 gcagggcgtc tggtatcgcg gcagcgcgac cgccgaggcg gcgcgcctcg gcctctcggg
     1561 ctgggtgcgc aaccgcagcg acggaacggt cgaggcgctc gcgatcggcc cgctcgtcaa
     1621 ggtcgaaacc ttcgtcgcct gggcccaccg gggcccggag aatgcgcgcg tgtcgcgggt
     1681 ggaggtgttc gaggccgaac tcgagcgcct caccggcttc gagcagcgtc cgacggtttg
     1741 agcacagccc gacccgaacg aagcgccctc gacttccgcg acggcgacgc ggcgcgcccg
     1801 ttgcgcgccg tgggagttca tgcgctgtga tcgcgacttg cgtcgctcct acgaaaaccc
     1861 gcagaccccc agcgcatgtt cgattcccgt gggagcgacg caagtcgcga ttgcagcgcc
     1921 tccattccac gccgcagtcg tcaccgcacc gggcggcgcc tcaggggtac accacctcga
     1981 ccgcctcgcc cggcagccat tcgcgcaggc tctccagcag cgcgtcgtcc aggcgcacgc
     2041 gccagccgtc gccgagcggg agctcggctt cggcggcggc gttgcgatag cggacgcgga
     2101 tcgggcagcc gccctcgcgg aagggctcga gcagggtctg cagcttgccg gccgcgcccg
     2161 ccgcaccgcc gctggcggca agctcgccgt cgatgcgtag cgacagcgcg cgtgcgaaac
     2221 ggctgcgcgc ctcgccgagg gtgagcaggc ggtcggcgtt gatgcgcagg ccaccggaga
     2281 aatcgtcgtt gctcaccttg gcctccacca ccagcacctc gtcggtgacg atcttgccgc
     2341 ggctcgcctc cagcacctcg gaatacaccg tgacctcgcg cggctcggta ccgtcgtcga
     2401 gcagcacgaa ggccatcttg ccgcggttgc cgaccttggt gcgctgctcc atcaccacgc
     2461 cggccagcat ggtgatgtcg cgcgagggct cgagctgcgc cagcgtgcgg cgcacgaagc
     2521 ggcgcacctc gggcttgaag gcgttgaagg gatggccgga gaggaagaag ccgatcgcga
     2581 gcttctcttc cttgaggcgc tcgcgctcgg tccacgcacg caccttggcg aactcgggcg
     2641 ccgcgcccgc ggcctccggc atcaggtcga agagcccacc ctgcatcgcg ttggccgccg
     2701 cctgttcggc cgcctccatc gccagcgcga cggtcgccat cagctgcgcg cggtccaggc
     2761 ccttgtggcc cggcagggtg tccatcgcgc cggcgcggat cagcgcctcg atgacgcggc
     2821 ggtttaccat gcggcgatcg acgcgctcgc agaaatcgaa caggtcgcgg aagtctccgc
     2881 ccttctcgtg cgcggcgagg atcgcgcgca ccgcaggctc gcccacgccc ttcaccgcgc
     2941 ccaggccgta gcggatgatc ttgcgatcca ccggcacgaa gcggtagtcg gaggcgttga
     3001 catccggcgg cagcaccttg atgccgttgg cgagcgtgtc ttcgtagaag atcttgacgg
     3061 tatcggtgtt gtccaggtcg gacgacatcg tcgccgccat gaaggccgcg cagtggtgcg
     3121 ccttgagcca ggcggtgtgg taggtgacca ccgcgtaggc cgcggtgtgc gacttgttga
     3181 agccgtactc cgcgaacttg gtcatcaggt cgaagagctg ctcggcgagc gccgggtcat
     3241 agcccttctg cttcgcaccc tcggcgatgg tggcgcggtg cttggccatc tcgtcggcct
     3301 tcttcttgcc catcgcgcgg cgcagcatgt cggcaccgcc aagcgtgtag cccccgatga
     3361 tctgggagat ctgcatcacc tgttcctggt acacgatcac gccgtaggtc ggctccaggc
     3421 aggccttgag gtcggggtgg aagtagtcga tctcctgctg gcccttcttg cgcaggatga
     3481 agtcgtccac catgcccgag ccgagcgggc cgggacggta gagcgcgagc accgcgatga
     3541 tgtcctcgaa gcggtcgggc gcgagcttct tgagcagctt cttcatgccg tccgattcga
     3601 cctggaagat cgccgtggtg ttggcgtcct tgaggatctg gtaggcggcg ggatcctcga
     3661 agcccaggct catcaggtcc ggacggctgc cctccaggcg cgccacgtac tccagcgcga
     3721 gctcgatgat ggtcaggttg cgcaggccga ggaagtcgaa cttgaccagg cccacggctt
     3781 cgacgtcgtc cttgtcgaac tgcgacaccg gcgtggcgtc gtcgccgtcg gcgatgtaga
     3841 gcggacagaa gtcggtgagc ttgccgggcg cgatcagcac gccgccggcg tgcatgccga
     3901 cgttgcggct caagccctcc agcggctgcg ccaggctcca caggtcgcgc accgactcgc
     3961 cgtcgttgcc gtcggccatc atctcgccga tctgcggctc catctcgtag gccttgttca
     4021 gcgagacggg cttggcgccc tcgatcggga tcagcttgga gaggcggtcg cacaggccgt
     4081 aaggcagatc cagcacgcgg ccgacgtcgc gcaccaccgc cttcgaggcc atggtgccga
     4141 aggtggcgat ctggctcacc gcgtccttgc cgtagcgctc gcgcacgtac tcgatgacgc
     4201 ggtagcggtt gtcctggcag aagtcgatgt cgaagtcggg catcgacacc cgctcggggt
     4261 tgaggaagcg ctcgaacagc agcgcgtact cgagggggtc gatgtcggtg atcttgagcg
     4321 agtaggccac cagcgagccc gcgccggagc cccgccccgg tccgaccggc acgccgttgt
     4381 tcttgcccca ctggatgaag tcggccacga tcaggaagta gccggggaag cccatctgga
     4441 tgatggtgtc ggtctcgaac ttcagccgct gctcgtagcg cgggcgctga cgctcgcgct
     4501 cctccgggtg cgggtagagc tgggcgaggc gctcctccag ccccttcttc gcctccgcga
     4561 cgaggaagtc gtcgagcgtc atgccctcgg gcgtcgggaa caagggcagg aagttcttgc
     4621 cgagttgcac ggtgagcgag cagcggcgcg cgatctcgac cgcgttctcg agcgcctcgg
     4681 gcagatcggc gaagagctcg cacatctccg cctggctctt gaggtactgc tcctcggtga
     4741 agtcgcgcgg ccggcgcttg tcggcgagca cgtagccctg cgcgatgcag acgcgcgcct
     4801 cgtgtgcctt gaaatcctcg cgcttgagga actgcaccgg gtgggtcgcc accaccggca
     4861 ggccgagccg gccggcgagt tccaccgcgt ggcggatgta ggactcggtg ccgggatgcc
     4921 cggcgcgctg gatctcgatg tagaaggcgt ccggaaacag ccgcgcccag tcggccgcga
     4981 gctgttcggc gagcgcgagg ttgccggccg cgatcgccgc gccgatgtcg ccgctcatcg
     5041 cacccgacag gcacagcagc gcgcttgcgg caccattctc gaaccactcg cggcgcatct
     5101 cggcgcggcc gcggtgcttg ttctcgaggt aggcgcgcgt cagcagctcg cacagctgac
     5161 catagcccgc gcggttcttg cagatgagca gcacgcgctg cggcttgtcg cgctcggcct
     5221 cgttgcggat ccaggcgtcg acgccgacga tgggcttgat gcccttgccg cgcgcgccct
     5281 tgtagaactt gaccatgccg aacaggttgg cgagatcgga gacgccgagc gccggcatgc
     5341 cgtcggccgc ggcggcggcg atcgcctggt cgagctggac gatgccgtcg gtgatcgaat
     5401 attcggagtg caggcggaga tgcacgaagc gcggagcgct cggcacgatg ggcgcttcgg
     5461 ccgtggaggg ggcagcagga ttcatggcgc ggattttacc cgacgccgcc cgcccccgcc
     5521 gccgacactc agggcaacag caccgtcgag ccggtggtct tgcgcgcctc gaggtcgcgg
     5581 tgcgcctggg ccgcgtcctt cagcgcatag cgctgcagga tctcgatcct cacctggccg
     5641 ctgcgcacca cctcaaacag cgccgcggcg cgttcgacga gctccgcgcg gctggcgatg
     5701 tagtcgggca gcccgggccg agtgacatag accgagcccg ccttgtgcag caaggcgcaa
     5761 tcgaaggcgg cgaccggacc ggtggcgttg ccgtagctca ccatggtgcc gcggcggcgc
     5821 agacaggcga ccgaatcgag gaaggtgtcg cgcccgaccg aatcgtacac caccgcgacg
     5881 ccttcgccgc cagtgagcgc gcgcacgcgc tcggggaagt tctcgcgcga gtacacgatg
     5941 acgtggtcgc agccgagcgc gcgcgcgcgc tgcgccttgt catcgttgcc cacggtgccg
     6001 atcacggttg cgccgagctg cttcgcccac tgcaccagga tcgagccgac cccacccgcc
     6061 gccgcatgca ccaggatggt ctcgcccgcc ttgaccgggt aggtggcgtg caacaggtat
     6121 tgtgcggtaa tgccctgcag catcatcgcc gcgccctgct cgaaggagat gtcgtccggc
     6181 aacggcacca ggtggcgcgc ctcgatgttg cgcgcctcgg cataggcatc gagcgggccg
     6241 ctggtatagg ccacgcggtc gcccaccttc agctcggcga caccgtcgcc gaccgcctcc
     6301 accacgcccg ccccctccat gcccagcccg gacggcagcg tcagcgggta caggccactg
     6361 cggtgatagg tgtcgatgaa gttgagcccc accgcatggt ggcgcaggcg cacctcgccg
     6421 gtcgcgggtg gcggcagctc gacggcctcc cactgcagga cttcggggcc gccggtgcgg
     6481 tggattcgga tcgcatgggt catcggattc tcctcgggat tccttgcgcg cagcgtcgga
     6541 gggagcccgg ccacgcggcg tcgggtgatc ttagcgtacc agcgagaccc cgatgcggta
     6601 ctgcgcttcc ttgttctcgt cgtactcgag cagggtctcg ccgtagccct tgaaggcctg
     6661 cagatggacg aaggcgccca ccccggagaa cacgctgcgc cggatcggat aggacaggtc
     6721 gtactgggtg cccatcttgc cctcggtgcc gcgccggatc agcgtggaga acagccagcc
     6781 gtcgtcgcgg ccgaggcgca ggccgagctc ggcgtggccg cggtagcgcg cgatgtcggg
     6841 attgtcctcc cgatcgaggt aggtccacgc cttgggcacg acgccgaggt aggagccgga
     6901 ctcgtccagg cgcaggcgcg cgtcggcgcg cacgaagagc gtgtcgatgc tgcgcgaggg
     6961 catgtcctcc ttgccgttcg attcgtgctc gtaaccgccg gagagtgcca gcgaaccgcg
     7021 cttttgcggg tcgtcgagcg cgaagcggta gaacagcgaa gggcggaagc tcgaatcgcg
     7081 gaatggcttg gattccgact gcaggtccca cagcgaggtc tgggtgaagc cgaagtagag
     7141 cccgctcgcc accgggatcg tctccgccac cacgccctgc tcgtcgaaga tgcggtaacg
     7201 gaagctgaac tggaagcgcg ccgacaccgg gtcgcgtccg ccgatgagga agtacatcgg
     7261 ctcgtggaag ccgaagcccg acggctcgac cggcgccgca tcggacggca ccgccgccac
     7321 cggcgccatc ggcgccaccg cggccgctgg ggcgaccgcc gtcgtgccgg ccgcggcgac
     7381 cggcgggatg gcggtcgacg cggtggccgc cgtagccaca ccggccgccg ccccggcgtc
     7441 gagcagcacg cgggccgaag gccgcccgac gagcgcgagc gccgccaccc cggtgacctc
     7501 catcggccag cgtgcgaaat agcggcgctg gccgcccccc gccgcgggcc tgccgatcgc
     7561 caccagctcg accgcgatgc gcggcccgcc acccgacagc tcgatgctcg ccggcagccg
     7621 cgccggccaa tccgccgcgc ggccctcgcc gatcaccacg acttcgaagc gctggcccgg
     7681 cacgacctgc gggctggagg tggcgagcag ccagtcggcg gcgaacgcgg ccggcgcggc
     7741 cagggcgagc gcgccggcga gcagcagcgg gcgcggggag gaagttttcg gcatggcgat
     7801 cgcctggcgg gaacgaagga aacaaagcgg ggcagcccct cgcaggactg cccccgggaa
     7861 aacgccgcgc gaacctcctc gtgcgaggct cgtgcggagg cggcgccgag cggcgccgcc
     7921 ggcaggcatc aggccccggc gttgcgcgac tccttggcat cgaccacggc gagcgcggtc
     7981 atgttgacca ggcggcgcac ggtggccgag ggggtcacga tgtgcaccgg tttggccgcg
     8041 cccagcagga tcggacccac cgagacgccg tcgccattgg ccaccttcat caggttgaac
     8101 gagatgttgg cggcgtccag gttgggcatc accagcaggt tggcctcgcc cttcaggccc
     8161 gaatccggat gcagcttgtc gcggatctcc tcggagagcg cggcatcgcc gtgcatctcg
     8221 ccatcgacgt tgaggtcggg cgaggtctcg cgcaggatct cgcaggcgcg gcgcatcttg
     8281 cgcgccgaag ccgagttcga gctgccgaag ttggaatgcg acaacagcgc cacgctcggc
     8341 tcgataccga agcggcgcag ttcgtcggac gccatgcggg cgatctcggc gacttcctcc
     8401 gcactcgggt tctcattcac ataggtgtcg gtgatcgcca gcatgcgctt ggtgagcagc
     8461 agcaggttca tcgccgcgaa ctgcttggcg cccggcgcca gcccgatcac gtccgccacc
     8521 tgcttgaggt ggtagtcgta ggagccgaag gtgccgcaca ccagcgcatc ggcatcgccg
     8581 cggcgcagca tcatgacccc gatcagggtg gtgtcgcggc ggatcttggc tttggccagg
     8641 tcgggggtga ccccgtcgcg cttcatgagc ttgtagtact cggtccacag ctcgcgatag
     8701 cgcgggtcgg actccggatt cacgatctcg aagtcgcgct cgggcttgag gttgaggccg
     8761 atcttcttga tccgcatctc gatgacctcg gggcgaccga tgaggatcgg acgcgccaag
     8821 ccttcgtcca gcaccacgcg caccgcgtgc agcacgcgct cgtcctcacc ctcggcgtag
     8881 atcacgcgct tctgctccag cggcaccgcc ttggcggccg agaacaccgg cttcatgacg
     8941 atgccggact ggtagacgaa ggcgttgagg ctggaggcgt aggcctcgag gtcggcgatc
     9001 ggcttggtcg ccacgcccga atccatcgcc gccttcgcca ccgcgggcgc gatcttgacg
     9061 atcaggcgcg gatcgaaggg cttcgggatg atgtactcgg aaccgaagct gagctcctgg
     9121 ccgccgtagg cgctggcgac gatgtcgctc gcctcggcct cggccagctc ggcgatcgcc
     9181 ttcacgcagg cgagcttcat ctcctcgttg atcgtggtcg cgccgacgtc gagcgcaccc
     9241 cggaagatga aggggaagca cagcacgtta ttgacctggt tcgggaagtc cgaacggccc
     9301 gtggccacga tgcagtcggg acgcacggcc ttggcgtcgg ccggcaggat ctcggggttc
     9361 gggttggcga gcgcgaagat cagcggcttg gcagccatct tcgccaccat ctcgggcttg
     9421 agcacgcccg cggtcgacaa gcccaggaag acgtcggcgc cggcgatcac gtcgcccagc
     9481 gcgcggccct cggtcttctg cgcgtagcgc gccttggtcg gctccatgtt gggctcgcgg
     9541 ccctcgtaga tgacgccctt ggagtcgcac acatagatgt tctcgcggcg caggcccacg
     9601 ctgaccatca tgtcgaggca ggcgatcgcc gcggcaccgg cgccggagca caccagcttg
     9661 acgtcggcga tgttcttgcc gaccaccttg agaccgttga ccaggccggc agccgaaatg
     9721 atcgcggtgc cgtgctggtc gtcgtggaag acggggatct tcatgcgctc gcgcagcttc
     9781 ttctcgatgt agaagcactc gggcgccttg atgtcctcga ggttgatgcc gccgagcgtg
     9841 ggctcgagcg cggcga
//
LOCUS       DAOQBS010000113         9731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_6352_length_9731_cov_16.852212, whole genome shotgun sequence.
ACCESSION   DAOQBS010000113 DAOQBS010000000
VERSION     DAOQBS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6352_length_9731_cov_16.852212"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /gene="prfB"
                     /locus_tag="PKN25_11875"
     CDS             complement(join(<1..915,917..997))
                     /gene="prfB"
                     /locus_tag="PKN25_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239085.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNV91640.1"
                     /translation="MEAERQNAIAEKLADLAARGRELRRYLDYDVKASKLEEVNRALE
                     DPAVWNNAERAQELGKEKRQLEDVVLTLQKIDAQSRDLKDLFELAEAEDDDDTFEAVE
                     ADLVELQGAIEKLEFRRMFSNPMDPNNCFIEIQAGAGGTEAQDWAGMLERMYLRYGEK
                     KGFTVELMEETEGEVAGIKNCTIKFSGEYAYGYLRTETGIHRLVRKSPFDSNARRHTS
                     FTSVFVYPEVDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHKNKAEAMSMLKARLYEAELRKRQSEQQKLEDSKSDIGWGHQIRSYVLDQ"
     gene            1246..3900
                     /locus_tag="PKN25_11880"
     CDS             1246..3900
                     /locus_tag="PKN25_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV91641.1"
                     /translation="MQHNSRKILGVLIATVLALNAFIAGLLGYAMLEAKARKEREVRT
                     TVENLALLLDQSVGSSVREIDLLLRGAQNRIERALRDKGRLERDDFDSVIDVHRDGLS
                     QTAQVRVSDATGTVVLGDQVTAETRTSYADRPFFIDHRALDDGEMQASKLLHGRISNT
                     WIVVFSRRYNAPDGDFAGVVTAAVPADHFQRLLSGLQLGPHGIALVRDLDMTLIARWP
                     RLDAPAGVIGSRGGSQELNEIVASDVRAQSFYSARTADGVARINAYRRLERLPAFVVV
                     GWGEKDYLAQWKEDVGKAMLLWALFLGVTGLAAWLLYRLVDANDRANERSRILLQNAS
                     DGIHITDAEGIVIEASDAFCRMLGRSRSEVIGMRAGSWDACHPEPDMRALISELFASS
                     EVHTFETAHRRKDGSVYPVEVTAFPLEVDGRTVLFNSARDISARKSAEAEVRKLAYFD
                     ALTGLPNRRLLMERLERAQASCARQGTVGALLFIDLDNFKAVNDTVGHQEGDRLLVQV
                     AAILVRCVRKDDTVARLGGDEFIVMLEELGGEGREAAEQAAAVGSKILAALNQRFQLG
                     SSEHRTSVSIGVSLFGELADEPVDEPLRRADMAMYRAKLGGRNAVCFFDPSMLADVRA
                     RADVEAGLWIALEQRQFVLHYQPQVAADGAVLGVEALVRWHHPVRGLVPPGEFIPLAE
                     ECGLILPLGRWVLEAACAQLAAWADDPWRSRLVIAVNVSARQFLHEDFVAGVVAALEC
                     SGADPGRLDLELTESSLVTDVDKVSEKMEALKALGVGFSLDDFGTGYSSLAYLKRLPL
                     DQLKIDRSFVRDILVDPNDAAIARMIIVLADNLGLRVIAEGVECEEQRDFLARNGCST
                     YQGYLFSAPQPIDALEAFLDARRSRPAR"
     gene            complement(3909..5021)
                     /locus_tag="PKN25_11885"
     CDS             complement(3909..5021)
                     /locus_tag="PKN25_11885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNV91642.1"
                     /translation="MKIFKLPDLGEGLQEAEIVEWHVKAGDEIAADQPLVSVETAKAI
                     VDIPSPYDGRVAKLYGEVGQLVHVGAPLAGFEGGAETADTGTVVGEMQVGQEVRAEAP
                     AALAPAAHGGAGIRATPAVRALARQLKVELEMVTPSGADGVITREDVQRAARVLAEAG
                     PAEALRGVRRAMAQNMSLAQSEVAGATVIDDAVIHAWPPGTDVTVRLIRALVAGCRAA
                     PALNAWYESHAMARRLVPQVHVGIAVDLPDGLFVPVLRDVANRSPDDLRQGLERMRAD
                     VRARRIPPEEMRGHTITLSNFGMIGGKYASPLVVPPTVAILGAGRVHEQVLAVDGAPA
                     VRRVLPLSLSFDHRVVTGGEAARFLKAVIDDLERAD"
     gene            complement(5018..5998)
                     /locus_tag="PKN25_11890"
     CDS             complement(5018..5998)
                     /locus_tag="PKN25_11890"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNV91643.1"
                     /translation="MAEINLVEAVNLALAHELARDEAVVLLGEDIGANGGVFRATVGL
                     QQRFGAGRVVDTPLAETAIVGTAIGMAAMGLRPVAEIQFSGFLYPAIDHLINHAARLR
                     NRTRGRLTCPLVVRTPCGGGIHAPEHHSESPEAMLAHTPGLRVVMPSSPARAYGLLLA
                     AIRDPDPVMFLEPTRMYRLFRQEVADDGEALPLDVCFTLRGGTDLTLVSWGAMLHETQ
                     AAADALATEGISAEVIDVATLKPLDLPTILESVARTGRCVIVHEAARTAGLGAEIAAG
                     LAEEGLYSLLAPVQRVTGYDTVMPLSRLETQYLPSVERIVAAARKTLEAS"
     gene            complement(5991..7094)
                     /gene="pdhA"
                     /locus_tag="PKN25_11895"
     CDS             complement(5991..7094)
                     /gene="pdhA"
                     /locus_tag="PKN25_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNV91644.1"
                     /translation="MSTVARFQIDHVRFLDPQGEALGPLPDFARDPAQLVPMYRAMYL
                     TRTFDAKAIALQRTGKLGTFASALGQEAIGVGVASAMRAEDALFPSYREHAAQLVRGV
                     RIEESLRYWGGDERGSDFAQARHDFPNCVPIGTQVCHAAGAAYAFKLRGEARAAVCFL
                     GDGGSSKGDFYEGLNFAAVWKAPLLIVVSNNQWAISVPRSAQSATATLAQKAIAAGVD
                     GVQVDGNDLVAVHHVAREALAKARAGGRPTLIEAVTYRLGDHTTADDASRYRDPAVVQ
                     EQWSFEPLTRLRNHLVGLGAWGPDKEEALARECTETVSAAVEAYLNTPPPTTAAMFEH
                     LFATLPAALVAQRDEALRFAPRKAGGQPGGDHG"
     gene            complement(7284..7964)
                     /locus_tag="PKN25_11900"
     CDS             complement(7284..7964)
                     /locus_tag="PKN25_11900"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNV91645.1"
                     /translation="MSAQASDWRVPAATLRWLDAVPAERGVAMLVRHSVRDPLPPGDL
                     GYANALNAEGVELARSLGERLHGRLRSLHTSPLQRCVHTAEVFARVAGDTPPIVTDRM
                     LGDPGAWVLDRALAGENWKRLGSEGIMEQLATTDEDLPGTRPARVGAQNLAMHMLAIA
                     AGSPGLHVFVTHDILLAIAAARFLGPPPAGPERWPGYLEAAFFWQDDDGLHAGYRDRH
                     RGGLTGAR"
     gene            complement(7981..9672)
                     /gene="recJ"
                     /locus_tag="PKN25_11905"
     CDS             complement(7981..9672)
                     /gene="recJ"
                     /locus_tag="PKN25_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNV91646.1"
                     /translation="MTRIQPRSIPQQAFARLADAGLHPLLARLYAARGIARADELDTS
                     LKNLLPPEALTGTAEAAILLADAIEAGARMVIVADYDCDGATACAVGVRALRAFGADV
                     HYLVPDRVTLGYGLTPAMVEIAARLEPDVLITVDNGIASVEGIAAARAHGMATVITDH
                     HLPGDVLPEADVIVNPNQPGCDFPSKALAGVGAMFYTMLALRAELRERGAFAGAKEPN
                     LADLLDLVALGTVADVVKLDRNNRILVAQGLARMRAGRLQPGIRALFGLAGRDPARAS
                     TMDLGFMIGPRLNAAGRLSDMSLGIECLITDDPGRAMNIAQELDKLNRERRSIEAGMQ
                     EEALARLAGFDAGNRATVALFEPDWHQGVIGIVAGRIKERLHRPTIAFARASDGELKG
                     SGRSIPGLHLRDALDLVTKRQPDLIVRFGGHAMAAGLTIRESELARFDAAFEEVVGEL
                     LEPAQLERRIDTDGNLESGYFALDAARMLDNEIWGQGFPAPLFDDVFRVERQRLLKDK
                     HLKLELSRGSTRHEAIRFNHAEGAAGQIHAAFRLGINEYNGVASVQLMLEHFEAA"
BASE COUNT         1333 a         3260 c         3609 g         1529 t
ORIGIN      
        1 ctggtcgagc acgtagctgc ggatctggtg accccagccg atgtcgctct tggagtcctc
       61 gagcttctgc tgctcggact ggcgcttgcg cagctcggcc tcgtacaggc gcgccttcag
      121 catcgacatg gcctcggcct tgttcttgtg ctgcgagcgg tcgttctggc actgcacgac
      181 gatgccggtg ggctcgtggg tgatgcgcac cgcggagtcg gtcttgttga tgtgctggcc
      241 gcccgcgccc gaggcacggt aggtgtcgat gcgcaggtcg gccgggttga tctcgatctc
      301 gatcgagtcg tcgacctccg gatacacgaa caccgaggtg aagctggtgt ggcggcgcgc
      361 gttggagtcg aacggcgact tgcgcacgag gcggtggatg ccggtctcgg tgcgcaggta
      421 gccgtaggcg tattcgccgg agaacttgat cgtgcagttc ttgatgccgg cgacctcgcc
      481 ctcggtctct tccatgagct cgacggtgaa gcccttcttc tcgccgtagc gcaggtacat
      541 gcgctcgagc atgcccgccc agtcctgcgc ctcggtgccg ccggcgccgg cctggatctc
      601 gatgaagcag ttgttggggt ccatcgggtt gctgaacatg cggcggaact cgagcttctc
      661 gatcgcgccc tgcagctcca ccaggtcggc ctcgaccgcc tcgaaggtgt cgtcgtcgtc
      721 ctcggcctcg gcgagctcga acagatcctt gaggtcgcgc gactgcgcgt cgatcttctg
      781 cagcgtcagc accacgtcct cgagctggcg cttctccttg ccgagttcct gcgcgcgctc
      841 ggcgttgttc cacaccgccg ggtcttcgag cgcgcgattg acctcttcga gcttcgatgc
      901 cttcacatcg tagtcaaaga tacctccgta gttcgcgacc ccgggcggcg aggtcggcga
      961 gtttttcggc gatggcgttc tggcgttcgg cttccatggc gaggctccgg cggaatctgg
     1021 gaaaccgcac attataccgg ttaccggagc agcgccgtcg gctgcagcgc agcttcatga
     1081 gcagggacgt cccgccgact ccgcgcagac gcagcgagag cctcgttcgc ctcccgcttc
     1141 caggctggag tacctgcagg cgggacggtt acaattacgc ccaattcagg atccgcgtgc
     1201 cgtcatcgac ccggggaacg cggtaggaac tcgtctggcg ggcacatgca gcacaacagc
     1261 aggaaaatcc tcggggtatt gatcgccacg gtgctggcgc tcaacgcatt cattgcggga
     1321 ctgctgggct atgcgatgct ggaggcgaag gcacgcaagg agcgggaggt gcgcaccacg
     1381 gtcgagaacc tggccctgct cctcgaccag agcgtcggtt cctctgtccg cgagatcgac
     1441 ctcctgctgc gcggagcgca gaaccggatc gagcgtgcgt tgcgcgacaa ggggcgcctg
     1501 gagcgcgacg atttcgactc ggtgatcgac gtgcatcgcg acgggctgtc gcagaccgcc
     1561 caggtccggg tcagtgatgc caccggcacg gttgtcctcg gcgaccaggt gacggccgag
     1621 acgcgtacaa gctatgccga ccgcccgttc ttcatcgatc atcgcgcgct cgacgatggc
     1681 gagatgcagg ccagcaagct gctgcacggg cgcatttcaa atacgtggat cgtggtgttc
     1741 tcgcgccgct acaacgcgcc cgatggcgac ttcgcgggcg tcgtgaccgc agccgtgccg
     1801 gccgatcatt tccagcgcct gctttcgggc ctgcagctcg ggccgcatgg catcgccctg
     1861 gtgcgcgacc tcgacatgac gctgatcgcg cgctggccgc gtctcgatgc cccggccggc
     1921 gtgatcggca gtcgcggcgg gtcgcaggag ctcaacgaga tcgtcgcctc cgacgttcgc
     1981 gcgcagtcct tctatagcgc gcgcacggcc gacggcgtgg cgcgcatcaa tgcctaccgc
     2041 cggctcgagc gcctgccggc cttcgtggtc gtcggctggg gcgagaagga ctacctcgcg
     2101 cagtggaagg aggacgtcgg caaggccatg ctgctgtggg cacttttcct cggcgtgacc
     2161 gggctggccg cctggctgct gtatcggctg gtcgacgcca acgaccgtgc caacgagcgc
     2221 agccgcatcc tgctgcagaa cgccagcgac ggcatccaca tcaccgatgc ggagggcatc
     2281 gtcatcgagg ccagcgacgc cttctgccgc atgctcgggc gatcgcgcag cgaggtcatc
     2341 ggcatgcggg cggggagctg ggatgcctgc catccggagc ccgacatgcg tgcgctgatc
     2401 agcgagctgt tcgcctcgag cgaagtgcac accttcgaaa cggcgcatcg gcgcaaggat
     2461 ggaagcgtgt atcccgtcga ggtgaccgcg ttcccgctcg aggtggacgg caggacggtg
     2521 ctgttcaact ccgcgcgcga catctccgcg cgcaagtcgg cggaggccga ggtcaggaag
     2581 ctggcctact tcgacgcctt gaccggcttg cccaaccggc ggctgctgat ggagcggctg
     2641 gagcgggcgc aggcgagctg cgcgcgtcag ggcacggtcg gcgccttgct gttcatcgac
     2701 ctggacaact tcaaggcggt caatgacacc gtgggccacc aggagggcga tcggctgctc
     2761 gtgcaggtcg ccgccatcct cgtacgctgc gtgcgcaagg acgataccgt ggcccggctc
     2821 ggtggcgacg agttcatcgt catgctggag gaactgggcg gggaggggcg ggaggcggcg
     2881 gaacaggccg cggcggtggg cagcaagatc ctcgcggcgc tgaaccagcg tttccagctc
     2941 ggcagttcgg aacatcgcac ctcggtgagc atcggcgtga gccttttcgg cgagttggcc
     3001 gacgaaccgg tcgacgagcc cctgcgacgg gccgacatgg cgatgtaccg ggccaagctc
     3061 ggcggacgca acgcggtgtg cttcttcgac ccctcgatgc tggccgacgt gcgcgcccgg
     3121 gccgacgtgg aggcagggct gtggatcgcg ctcgagcagc gccagttcgt gctgcattac
     3181 cagccccagg tcgccgccga cggcgcggtg ctcggcgtgg aggcgctggt gcgttggcat
     3241 cacccggtgc gcggcctggt accgccgggc gagttcattc cgctcgccga agagtgcggg
     3301 ttgatccttc cactcgggcg ctgggtgctc gaggccgcct gcgcgcagct ggcggcgtgg
     3361 gcggacgacc cgtggcgcag caggctggtg atcgcggtca atgtcagcgc acgccagttc
     3421 ctccatgaag acttcgtcgc cggggtggtc gccgcgctcg agtgctcggg ggccgacccg
     3481 ggccggctcg acctggagct gaccgagagc tcgctggtga ccgacgtcga caaggtcagc
     3541 gagaagatgg aggccctcaa ggcgctcggc gtgggattct cgctcgacga cttcgggacc
     3601 ggctattcct cgctcgccta tctcaagcgc ttgccgctgg atcagttgaa gatcgatcgc
     3661 agcttcgtgc gcgacatcct ggtcgatccc aacgacgcgg cgatcgcgcg catgatcatc
     3721 gtgctcgccg acaacctcgg cctgcgtgtg atcgcggaag gcgtggagtg cgaggagcag
     3781 cgcgacttcc tcgcccgcaa cggctgctcg acctatcagg gctacctttt cagtgcgccg
     3841 cagccgatcg acgcgctcga ggccttcctc gacgcccgaa ggtctcggcc ggcgcgctga
     3901 aggagcgctc agtcggcgcg ttccagatcg tcgatcaccg ccttcaggaa gcgcgcggcc
     3961 tcgccgccgg tgaccacgcg gtggtcgaag gacaggctca gcggcagcac gcggcgtacc
     4021 gccggggcgc catcgaccgc gagcacctgc tcgtgcaccc gcccggcgcc gaggatggcc
     4081 accgtgggcg gcaccaccag cggcgaggcg tacttgccgc cgatcatgcc gaagttggac
     4141 agcgtgatgg tgtggccgcg catctcctcg ggcggaatcc tgcgcgccct gacgtcggcg
     4201 cgcatgcgct cgaggccctg gcgcaggtcg tcggggctgc ggttggcgac gtcgcgcagc
     4261 accggcacga agaggccgtc gggcaggtcg accgcgatgc cgacgtgcac ctgcggcacc
     4321 agccggcgcg ccatggcgtg gctctcgtac caggcgttga gcgccggcgc ggcgcggcag
     4381 ccggcgacca gggcgcggat caggcgcacg gtgacgtcgg taccgggcgg ccaggcgtgg
     4441 atcaccgcgt cgtcgatcac ggtggcgccg gcgacctcgc tctgcgccag gctcatgttc
     4501 tgcgccatcg cccggcgcac gccgcgcagc gcttcggcgg ggccggcctc ggcgagcacg
     4561 cgcgcggcgc gctgcacgtc ctcgcgggtg atcacgccgt cggcgccgga gggcgtcacc
     4621 atctcgagct cgaccttcaa ctggcgcgcc agcgcgcgca ccgccggggt ggcgcggatc
     4681 cccgctccgc cgtgggctgc cggtgcgagc gcggccggcg cctcggcgcg cacctcctgg
     4741 ccgacctgca tctcgccgac cacggtgccg gtgtcggcgg tctcggcgcc gccctcgaag
     4801 ccggcgagcg gtgcgcccac atgcacgagc tggccgacct cgccgtacag cttcgcgacc
     4861 cgcccatcgt aaggggaggg gatgtccacg atcgccttgg cggtctcgac cgataccagc
     4921 ggctggtcgg cggcgatctc gtcgcccgcc ttgacatgcc attcgacgat ctcggcttcc
     4981 tgcagtcctt cgcccaggtc gggcagcttg aagatcttca tgatgcctcc agggtcttgc
     5041 gcgccgccgc gacgatgcgt tcgacgctcg gcaggtactg ggtttcgagc cgcgacagcg
     5101 gcatcaccgt gtcgtagccg gtgacgcgct gcaccggcgc gagcagggaa tacaggccct
     5161 cctcggcgag gccggccgcg atctcggcgc ccaggccggc ggtgcgcgcg gcctcgtgca
     5221 cgatcacgca acgcccggtg cgggcgaccg actccaggat ggtcggcagg tcgagcggct
     5281 tgagcgtggc gacgtcgatg acctcggccg agatgccctc ggtggcgagc gcgtcggccg
     5341 cggcctgggt ctcgtgcagc atcgcgcccc agctcaccag ggtgaggtcg gtgccgccgc
     5401 gcagcgtgaa gcacacgtcc agcggcagcg cctcgccgtc gtcggccacc tcctggcgga
     5461 acaggcggta catccgcgtc ggctcgagga acatcaccgg gtcggggtcg cggatcgccg
     5521 ccagcagcag gccgtaggcg cgtgcgggcg aggacggcat gaccacgcgc aggccggggg
     5581 tgtgggcgag catcgcctcc gggctctcgg agtggtgctc gggcgcatgg atgccgccgc
     5641 cgcagggggt gcgcacgacc agcgggcagg tgagccggcc gcgggtgcgg ttgcgcaggc
     5701 gcgcggcatg gttgatcagg tggtcgatcg ccggatacag gaagccggag aactggatct
     5761 ccgccaccgg cctgaggccc atcgccgcca tgccgatcgc ggtgccgacg atcgcggtct
     5821 cggcgagcgg ggtgtcgacc acgcgcccgg cgccgaagcg ctgctgcagc ccgaccgtgg
     5881 cgcggaacac gccgccgttg gcgccgatgt cctcgccgag cagcacgacc gcctcgtcgc
     5941 gcgcgagctc gtgggcgagg gccaggttga ccgcctcgac gagattgatc tcagccatga
     6001 tcgcctcccg gttgtccgcc cgccttgcgc ggcgcgaagc gcagggcctc gtcgcgctgg
     6061 gccacgagcg cggcgggcag ggtcgcgaag aggtgctcga acatcgccgc ggtggtgggc
     6121 ggcggggtgt tcagataggc ctcgaccgcg gcactcacgg tctcggtaca ctcgcgcgcg
     6181 agcgcctcct ccttgtccgg cccccaggcg ccgagcccga ccaggtggtt gcgcagccgg
     6241 gtcagcggct cgaaggacca ctgctcctgc accaccgcag ggtcgcggta gcgcgaggcg
     6301 tcgtcggcgg tggtgtggtc gccgaggcga taggtgaccg cctcgatcag ggtcggtcga
     6361 ccgccggcgc gggccttcgc cagcgcttca cgggcaacgt ggtgcactgc aacgaggtcg
     6421 ttgccgtcga cctgcacgcc atcgacgcct gcggcgatgg ccttctgcgc cagcgtggcg
     6481 gtggcgctct gcgcgctgcg cggcaccgag atcgcccact ggttgttgct caccacgatc
     6541 agcaggggcg ccttccagac ggcggcgaag ttgagcccct cgtagaagtc gcccttcgag
     6601 ctgccgccgt cgccgaggaa gcacaccgcc gcgcgcgcct cgccgcgcag cttgaaggca
     6661 taggccgcgc ccgcggcgtg gcagacctgg gtgccgatcg gcacgcagtt gggaaagtcg
     6721 tggcgggcct gggcgaagtc gctgccgcgc tcgtcgccgc cccagtagcg caggctctcc
     6781 tcgatgcgca cgccgcgcac gagctgggcg gcgtgctcgc gataggaggg aaacagcgcg
     6841 tcctcggcac gcatcgcgct cgccaccccg accccgatcg cctcctggcc gagcgcagac
     6901 gcgaaggtcc cgagcttgcc ggtgcgctgc agcgcgatcg ccttggcgtc gaaggtgcgc
     6961 gtgaggtaca tcgcgcggta catcgggacc agctgtgcgg gatcgcgcgc gaagtcgggc
     7021 agcggcccga gcgcctcgcc ctgcgggtcg aggaaacgga cgtgatcgat ctggaaccgg
     7081 gcgacagtgc tcatggcgcg ctcccttcag gctcggtcgg ccccgcatgc ctgcgcgccc
     7141 cggtcggggt ccgtctcctc tgggcgccgg tggcatccgg gcgtggatgt cgtcggcgac
     7201 cgggtggcgg cgccgcgacg ttttccgttt gcggggttgg agcgcgcccg tgcgcggatg
     7261 ttcccgacgc ggttgccgct tcgtcagcgc gcgccggtga ggccgccgcg gtggcggtcg
     7321 cgatagccgg cgtggaggcc gtcgtcgtcc tgccagaaga aggcggcctc gaggtagccc
     7381 ggccagcgct ccgggccggc cggcggcggg ccgaggaagc gcgccgccgc gatcgcgagc
     7441 aggatgtcgt gggtgacgaa gacgtgcaga ccgggcgagc ccgcggcgat cgcgagcatg
     7501 tgcatggcga ggttctgcgc gcccacgcgc gccggacgcg tgccgggcag gtcctcgtcg
     7561 gtcgtcgcca actgctccat gatcccctcg ctgccgaggc gcttccagtt ctcgcccgcc
     7621 agcgcgcggt cgagcaccca ggcgccgggg tcgccgagca tgcggtcggt gacgatcggt
     7681 ggcgtgtcgc cggccacgcg cgcgaagacc tcggcggtgt gcacgcagcg ctgcagcggg
     7741 ctggtgtgca ggctgcgcag gcgtccgtgc aggcgctcgc caaggctgcg cgcgagctcg
     7801 acgccttcgg cgttgagcgc gttggcgtag ccgaggtcgc cgggcggcaa ggggtcgcgc
     7861 accgaatggc ggaccagcat ggccacgccg cgctcggccg gcaccgcgtc cagccagcgc
     7921 agggtggcgg cggggacccg ccagtccgag gcttgcgcgc tcatcgcgat ggcggcgggc
     7981 ctacgccgcc tcgaaatgtt cgagcatcag ctgcacgctg gcgacgccgt tgtattcatt
     8041 gatgccgagg cggaaggcgg cgtggatctg gccggcggcg ccctcggcgt ggttgaagcg
     8101 gatcgcctca tggcgcgtgc tgccgcgcga gagctcgagc ttgaggtgct tgtccttcaa
     8161 caggcgctgg cgctcgacgc ggaagacgtc gtcgaacagc ggcgccggga agccctggcc
     8221 ccagatctcg ttgtcgagca tgcgcgcggc gtcgagcgcg aagtagcccg attccaggtt
     8281 gccgtcggta tcgatgcggc gctcgagctg ggcgggctcg agcagttcgc cgacgacctc
     8341 ctcgaaggcc gcgtcgaagc gtgcgagttc cgattcgcgg atggtcaggc cggccgccat
     8401 ggcgtggccg ccgaagcgca cgatgaggtc gggctggcgc ttggtgacga ggtcgagcgc
     8461 gtcgcgcagg tgcaggccgg ggatcgagcg cccggagccc ttgagttcgc cgtcgctcgc
     8521 gcgcgcgaag gcgatggtgg ggcggtgcag ccgctccttg atgcggccgg cgacgatgcc
     8581 gatcacacct tggtgccagt cgggttcgaa gagcgccacg gtggcgcggt tgccggcgtc
     8641 gaaacccgcc aggcgggcga gcgcttcttc ctgcatgccg gcctcgatgc tgcggcgttc
     8701 gcggttgagc ttgtcgagct cctgggcgat gttcatcgcc cggccggggt cgtcggtgat
     8761 caggcactcg atgcccaggc tcatgtcgga gagccgcccg gcggcgttga ggcgcgggcc
     8821 gatcatgaag ccgaggtcca tcgtgctggc gcgcgccggg tcgcgcccgg cgagcccgaa
     8881 cagcgcgcgg atgccgggct gcaggcgccc ggcgcgcatg cgcgcgaggc cttgcgcgac
     8941 caggatgcgg ttgttgcggt cgagcttgac cacgtcggcc acggtgccga gcgcgacgag
     9001 gtcgagcagg tcggcaaggt tgggttcctt ggcgccggcg aaggcgccgc gctcgcgcag
     9061 ttcggcgcgc agcgcgagca tcgtgtagaa catcgcgccc acgccggcca gcgccttgct
     9121 cgggaagtcg cagccgggct ggttggggtt cacgatcacg tcggcttcgg gcagcacgtc
     9181 gccgggcagg tgatggtcgg tgatcaccgt ggccatgcca tgggcgcgcg cggcggcgat
     9241 gccctcgacg ctggcgatgc cattgtcgac ggtgatcagc acgtcgggct cgaggcgcgc
     9301 ggcgatctcg accatggccg gggtgaggcc gtagccgagc gtgacgcggt ccggcacgag
     9361 gtaatgcacg tcggcaccga aggcgcgcag cgcgcgcacg cccaccgcgc aggcggtggc
     9421 gccgtcgcag tcgtagtcgg cgacgatgac catgcgcgcg ccggcctcga tggcgtcggc
     9481 gagcaggatc gcggcttccg ccgtgccggt gagcgcctcg ggcggcagca ggttcttcag
     9541 gctggtgtcg agctcgtcgg cgcgcgcgat gccgcgcgcg gcgtagaggc gggcgagcag
     9601 cggatgcagg ccggcatcgg cgaggcgggc gaaggcttgc tgcgggatgg agcggggctg
     9661 gattcgggtc atcggtcgaa gaaagcttgt aggtggccgg tgttcatgca caggccgcgg
     9721 cgcgtcggcg g
//
LOCUS       DAOQBS010000114         9710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_6374_length_9710_cov_15.333920, whole genome shotgun sequence.
ACCESSION   DAOQBS010000114 DAOQBS010000000
VERSION     DAOQBS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9710
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6374_length_9710_cov_15.333920"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PKN25_11910"
     CDS             complement(<1..920)
                     /locus_tag="PKN25_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNV91647.1"
                     /translation="MADDAPDHVHDLLIVGAGPVGLALALALKDAGLDIVLADARARE
                     AVARDPRDLALAHGTRLTLQRLGVWDGLPTTAIQHIHISHQGGLGRTLIDAAEHELPA
                     LGYVASAGALATALRKAVDAAGIPVFDETEITNLAAGEDDVIASLADTGRPAPTLRAR
                     LAACAEGGLRAGDPNVVEHDYGQHALIADVQVAGGHRHTAFERFTPQGPVALLPKGEG
                     YALVHVARPETADELLALDDAAYLARLQAHIGGRARLTGVGPRLRYPLVLRYRRSTIA
                     QRTVWLGNAAQTLHPVAGQGFNLALRDVWAL"
     gene            complement(913..2256)
                     /locus_tag="PKN25_11915"
     CDS             complement(913..2256)
                     /locus_tag="PKN25_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNV91648.1"
                     /translation="MNAPGTAPMDTTPFRARRARLLQRMQAAGGGVAILPTAPERVRN
                     RDAHYAYRHDSYFYYLSAFREPEAVVVLVAGKETKQILFCREKNEEREIWDGYRWGPE
                     AARAAFGFDEAWTIGDLEKRLPDYLADQPLLWTSLGYDNDWDARVLGALNAVRDKART
                     GLTPPHSVRDLRAELDEMRLVKDASELATMRQAAQISAAAHCRAMRATRPGRHEYEIE
                     AELLHAFRAAGSQAPAYTSIVAGGANACVLHYVDNDQRLNDGDLLLIDAGCELDGYAS
                     DITRTFPVSGRFSGPQRAVYELVLAAQAAAREATRPGAHWNQPHDAAVKVLAQGMLDL
                     GLLQGSLDGVLENGDYRRFYMHRTGHWLGMDVHDAGEYKLGGEWRPLVEGMVLTIEPG
                     CYIRAAEDVPEAFWNIGIRIEDDAIVTADGCALITEDAPKAVADIEALMRDARHG"
     gene            complement(2265..2984)
                     /locus_tag="PKN25_11920"
     CDS             complement(2265..2984)
                     /locus_tag="PKN25_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNV91649.1"
                     /translation="MILAAGRGERMRPLTDHTPKPLLEAGGKPLIVWHIERLRAAGFR
                     DLVINHAHLGHRLEEALGDGAAFGVRIAWSPEASALETAGGIRHALPLLGDQPFVVVN
                     GDVFCDADFTSLRAAAEGLHADGPLAHLLLVDNPEHHPKGDFHLDAAGLLHAEGAPRL
                     TFSGLGAYHPALFAALADDTPAKLAPLLRAAMNAGQVTGRHHAGRWIDVGTPRRLAEL
                     DRELEGTGNAPPTRPRVRPVT"
     gene            complement(3064..4104)
                     /locus_tag="PKN25_11925"
     CDS             complement(3064..4104)
                     /locus_tag="PKN25_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNV91650.1"
                     /translation="MHRLEQLRTWLASVLPGQSFELAPASADASFRRYFRATFADGSP
                     SRVVMDAPPEKEDVRPWLHVAELFRAAGAHVPDVLAHDLDQGFLLLSDLGSTTYLQAL
                     KAQDDAPQAARTAHHLYADALGALAAIQCASRPGVLSEYDRALLSRELQLFPEWYIAR
                     HKGVALTEAETAMLNTAFERILAVNLAEPQVFVHRDYHSRNLMLLDPAEGRGANPGII
                     DFQDAVYGPISYDLVSLFKDAYIRWDEEFILDLLIRYWETAKKLGLPVRAEFSDFHRD
                     FEWMGVQRHIKVLGIFARLYHRDGKDGYLADMPLVMDYLRRACKRWRDLGPLLKLLDR
                     LEPEETQVGYTF"
     assembly_gap    4479..4578
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4579..6849
                     /locus_tag="PKN25_11930"
     CDS             <4579..6849
                     /locus_tag="PKN25_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HNV91651.1"
                     /translation="APAPAPAPAPAAVAKPPVVAAPAPAVERPALAPGETAVSALRIH
                     GERGIELVAEGEAELQRDDTLLTADRVTYREPTDEVLAEGHVVLSRGGDTIRGPKATL
                     VVGDRVGSFETPTYELSRARPPALAGDAPRTVAGSGEADLLRLEGENQYRLANATWTT
                     CSPQDPDWYIKARELELDYDREVGTAHASSIVFMDTPIFWMPWAEFPLSGQRQSGLLP
                     PTFGSSNKTGVDFTQPYYWNIAPNYDATLAPRIMTRRGVQFGGELRYLGEDSQGTTRL
                     EWMPNDKVTGEERRLGSIQHQQRFAPNFYGTLDLNAVSDDGYFEDLSSNIGVASRVNL
                     LREGRLIYTGGWWTASALAQRYQTLNPDPDVRNVEPYRRLPQLRLDAVRADLAGGMAG
                     LLETEFVRFAHRERDRDEAARVVAYPQLSLPMQGAAWFVTPKAGVHYTSYDIDRVAAT
                     SGKPESITRSVPIVSLDSGLFFERETNYFGSDYLQTLEPRLFYLRVPSREQSDIPLFD
                     TNRFDVGFAQIFAENRYSGSDRIGDANDLTAALTTRFIDAETGVERLRALIGQRYYFS
                     DQKVYLYDEDELRRAGTGNEILAGLGGRITSTVTVDSYAQYNTETSRTERLNASVRYQ
                     PGFARTLNLSYRYAPNLRVDQDIVGLEDIDVSGQWPLARQWYGVGRVTHSLKDDRITE
                     AVAGIEYDGGCWVFRTAVHRFAIDEDDVTNAIFIQLELNDLAGIGSNPLSLIKRSVPG
                     YGKINDSSANRVFGNN"
     gene            6880..8196
                     /locus_tag="PKN25_11935"
     CDS             6880..8196
                     /locus_tag="PKN25_11935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV91652.1"
                     /translation="MKRLLFRSRVAIAIGLAATTLALPVHSAPRAVEVDRIVAVVNNE
                     VITGLELRARIEQTRRQLARQGAQLPPEEVLQRQLLERLIVERAQLQLARESSLRVDD
                     VTLDRAIERIASNNKLSIDQLRATLEKDGVTWSRFRDEIRSEILLTRLREREVDSRIV
                     VTDAEIDNFIANNPDAFSGQEFAVAHILLRTPEGASPQQVEAVARRAEQVMARLRSGE
                     DFARVAAEVSDAPDGLQGGSLGWRPLDRLPALFADAVRRMRPGETSPVLRSAAGLHIV
                     RLVDARGGGAAAVQKLEQTRARHILIKTSEVLSDADAEARLLAIRERVVNGADFAELA
                     KASSADLSAARGGDLGWLNPGDTVPEFERAMNALRPGEVSAPVRSPFGWHLIQLVERR
                     MQDVTDERKRAAARQALRERKAEQAYEDWLRQLRDSTYVDYRIERE"
     gene            8193..9209
                     /gene="pdxA"
                     /locus_tag="PKN25_11940"
     CDS             8193..9209
                     /gene="pdxA"
                     /locus_tag="PKN25_11940"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNV91653.1"
                     /translation="MSVAAEAGRRLVAITSGEPAGVGPELCLRLAERAWPLRPVVLAD
                     IGLMRARAVALDSAVGVVEFDAARPAAEGAVEVLHLPLAAPSRAGVLDPANGAYVLRL
                     LDRAIAGCLQGEFAAMVTAPVHKGVICEGLGAHETMPFTGHTEYLAEHTGTPRVVMML
                     VGGGLRVALATTHLPLAAVPAAITPQVLEETLRILHADLRRHFGLAAPRILVAGLNPH
                     AGEGGHMGREEIDVIIPVLERLRAEGMQLVGPLPADTLFVPHTLAQGDAVLAMYHDQG
                     LPVLKHASFGGGVNVTLGLPIIRTSVDHGTALDLAGSGRADPGSLFAALELAIAMTQA
                     RAAA"
     gene            9253..>9710
                     /locus_tag="PKN25_11945"
     CDS             9253..>9710
                     /locus_tag="PKN25_11945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HNV91654.1"
                     /translation="MHDFDTDGHRARKRFGQNFLSDPNIIRKIIDGIRPQPGELMVEI
                     GPGLGAMTDPLIERLGHLHVVEIDRDLIARLHERYTPAQLTVHEGDALKFDFGSLCAG
                     EEGGVGPRLRIVGNLPYNISTPILFHLAGFADQVKDMTFMLQKEVVMRMVA"
BASE COUNT         1369 a         3412 c         3457 g         1372 t
ORIGIN      
        1 agcgcccaca catcccgcag cgcgaggttg aagccctgtc ccgcgaccgg gtgcagggtc
       61 tgcgccgcgt tgccgagcca caccgtgcgc tgggcgatgg tcgagcgccg gtagcgcagc
      121 accaatggat agcgcaggcg cgggccgacg ccggtcaggc gcgcgcggcc gccgatgtgg
      181 gcctgcaggc gggcgaggta ggcggcatcg tcgagcgcca gcagttcgtc ggccgtctcg
      241 gggcgggcga catgcaccag cgcgtagccc tcgcccttgg gcagcagcgc caccggaccc
      301 tgcggggtga agcgctcgaa ggcggtgtgg cgatggccgc ccgcgacctg cacgtcggcg
      361 atgagcgcgt gctggccgta gtcgtgctcg accacgttgg ggtcgcccgc gcgcaggcca
      421 ccctcggcgc aggccgccag gcgggcgcgc agcgtggggg cggggcggcc ggtgtccgcg
      481 aggctggcga tcacgtcgtc ctcgcccgcg gccaggttgg tgatctcggt ctcgtcgaac
      541 accgggatgc cggccgcgtc taccgccttg cgcagggccg tggcgagcgc gcctgccgag
      601 gcgacgtagc cgagcgcggg cagctcgtgc tcggcggcgt cgatcagggt gcggccgagc
      661 ccgccctggt gcgagatgtg gatgtgctgg atcgccgtgg tcggcagccc atcccaaacg
      721 ccgaggcgct gcagcgtgag ccgggtgccg tgggcgagcg cgaggtcgcg cgggtcgcgc
      781 gccaccgcct cgcgcgcgcg cgcgtcggcg agcacgatgt cgagcccggc gtccttcaat
      841 gccagcgcga gcgccaggcc gaccgggccg gcgccgacga tcagcaggtc gtggacatgg
      901 tcgggcgcgt cgtcagccat gacgggcgtc ccgcatcagg gcctcgatgt ccgcaaccgc
      961 cttgggcgcg tcctcggtga tcagcgcgca gccgtcggcg gtgacgatcg cgtcgtcctc
     1021 gatgcggatg ccgatgttcc agaaggcctc gggcacgtcc tcggccgcgc ggatgtagca
     1081 gcccggctcg atggtcagca ccatgccctc gaccagcggc cgccactcgc cgccgagctt
     1141 gtattcgccg gcgtcgtgca cgtccatgcc cagccagtgg ccggtgcggt gcatgtagaa
     1201 gcggcgatag tcgccgttct cgagcacgcc gtccaggctg ccctggagga gaccgaggtc
     1261 gagcatgccc tgggcgagca ccttcaccgc ggcgtcgtgc ggctggttcc agtgcgcgcc
     1321 ggggcgggtg gcctcgcgcg ccgcggcctg ggcggcgagc acgagctcat agaccgcgcg
     1381 ctgcggacct gagaagcggc cgctcaccgg gaaggtccgg gtgatgtcgg aggcgtagcc
     1441 gtcgagctcg cagccggcgt cgatcagcaa caggtcgccg tcattgaggc gctggtcgtt
     1501 gtcgacgtag tgcagcacgc aggcattggc gccgccggcg acgatgctgg tgtaggcggg
     1561 ggcctggctg ccggcggcgc ggaaggcgtg cagcagctcg gcctcgatct cgtactcgtg
     1621 ccggcccggg cgggtggcgc gcatcgcgcg gcagtgggcg gcggcggaga tctgtgctgc
     1681 ctggcgcatg gtggcgagct cggacgcgtc cttgaccagg cgcatctcgt cgagctcggc
     1741 gcgcaggtcg cgcaccgagt gcggcggggt gaggccggtg cgggccttgt cacgcacggc
     1801 gttgagcgcg ccgagtacgc gcgcgtccca gtcgttgtcg tagccgaggc tggtccacag
     1861 caggggctgg tcggcgaggt agtcggggag gcgtttttcg aggtcgccga tggtccacgc
     1921 ctcgtcgaag ccgaaggccg cgcgcgcggc ctcggggccc cagcggtagc cgtcccagat
     1981 ctcgcgttcc tcgttcttct cgcggcagaa gaggatctgc ttcgtctcct tgcccgccac
     2041 cagcacgacc acggcctcgg gttcgcggaa ggcactgagg tagtagaagt agctgtcgtg
     2101 gcggtaggcg tagtgggcgt cgcggttgcg cacgcgctcg ggggcggtgg gcaggatggc
     2161 gacgccgccg ccggcggcct gcatgcgctg cagcaggcgg gcgcggcggg cgcggaaggg
     2221 ggtggtgtcc atgggggcgg tgccgggtgc gttcatggtt gaaattatgt cacggggcgc
     2281 acgcgggggc gggtcggcgg ggcgttccct gtgccctcca gttcccgatc gagttccgcc
     2341 aggcgccgcg gggtgccaac gtcgatccag cgcccggcgt ggtgccggcc ggtgacctgg
     2401 cccgcgttca tcgccgcgcg cagcaggggg gcgagtttgg cgggggtgtc gtcggcgagg
     2461 gcggcgaaca gggcgggatg gtaggcgccg agcccggaga aggtgagccg tggggcgcct
     2521 tcggcgtgga gcaggccggc ggcatcgagg tggaagtcac ctttcgggtg gtgctcgggg
     2581 ttgtccacca gcagcaggtg ggcgagcggg ccgtcggcgt gcaggccttc ggcggcggcg
     2641 cgcagggacg tgaagtcggc gtcgcagaac acatcgccgt tgaccacgac gaagggctgg
     2701 tcgccgagca gcggcagggc gtggcggatg ccgccggcgg tttccagcgc gctggcttcc
     2761 ggcgaccacg cgatacgcac gccgaaagcc gcgccgtcgc cgagcgcttc ttcgagccgg
     2821 tggccgaggt gggcgtggtt gatcacgaga tcgcggaagc cggcggcgcg cagtcgttcg
     2881 atgtgccaca cgatcagcgg cttgccgccc gcctcgagca gcggcttggg ggtgtggtcg
     2941 gtgagcgggc gcatgcgctc gccgcgaccg gcggcgagga tcatcgctcg ggtcatggtg
     3001 tctcggctga agggagcatg ggcaggcgca ggctggcgcc gcgttggggc cgcggtgcgc
     3061 cgttcagaag gtgtagccga cctgggtctc ctcgggttcg aggcggtcga gcagcttcaa
     3121 cagcgggccg aggtcgcgcc agcgcttgca ggcgcggcgc aggtaatcca tcaccagcgg
     3181 catgtcggcg aggtagccgt ccttgccgtc gcggtggtac aggcgcgcga agatgccgag
     3241 caccttgatg tggcgctgca cgcccatcca ctcgaagtcg cggtggaagt cggagaactc
     3301 cgcccgcacc ggcaggccga gcttcttcgc ggtctcccag tagcggatca gcaggtcgag
     3361 gatgaactcc tcgtcccagc ggatgtaggc gtccttgaac agcgacacca ggtcgtagct
     3421 gatcgggccg tagaccgcgt cctggaagtc gatgatcccg gggttggcgc cgcggccttc
     3481 ggcggggtcg agcagcatca ggttgcgcga gtggtagtcg cgatgcacga agacctgcgg
     3541 ctcggcgagg ttgaccgcga ggatgcgctc gaacgccgtg ttcagcatcg ccgtctcggc
     3601 ctcggtgagc gcgacgccct tgtggcgggc gatgtaccac tcggggaaga gctgcagctc
     3661 gcgtgacagc agggcgcggt cgtactccga cagcacgccg gggcgactgg cgcactggat
     3721 ggcggcgagc gcgccgaggg cgtcggcgta caggtgatgt gcggttcggg cggcttgcgg
     3781 cgcatcgtcc tgcgccttca gggcttgcag gtaggtcgtc gagccgaggt cggacagcag
     3841 caggaagccc tggtcgagat cgtgcgcgag cacgtccggc acgtgggcgc cggcggcgcg
     3901 gaaaagctcc gcgacgtgca gccaggggcg cacgtcctcc ttctcgggcg gggcgtccat
     3961 gacgacgcgc gagggactgc cgtcggcgaa ggtggcgcgg aagtagcgcc ggaagctggc
     4021 gtcggccgag gccggggcga gttcgaagga ctggccgggc aggacactgg cgagccaggt
     4081 ccggagttgt tcgaggcgat gcaaaccgga tctccgcggg ggttcgtgta caatccgcga
     4141 gtttatcaac acggtggttc gggtgggcag tcgcgcaccg cgaacgacgg caagggcttg
     4201 atccgcatgc ttccgaatac gcgcattcga ctgattccac tgctcctgct gggcctgtcg
     4261 ggcgctgcat tcgcgcaaac catgcccgcg cttcgggtgg cgcccgagct gctgcgccag
     4321 gcgtcgccgg ccacgccgga gcgacccgcg acgccggtgc aggcctcccc cgcggcggcc
     4381 tctgccacgc cacccccggc tgcacccgct ccgaccccgg cgcccgtctc cgcacccgcg
     4441 gccgtcgcac ccgcaccggc acccgcaccg gcacccgcnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnngc acccgcaccg gcacccgcac cggcacccgc ggccgtcgcc
     4621 aagccgccgg tggtcgccgc gcctgccccc gccgtcgagc gacccgcgct cgccccgggc
     4681 gagaccgcgg tcagcgcgct gcgcatccat ggcgagcgcg ggatcgagct ggtcgccgag
     4741 ggcgaggccg agctgcagcg cgacgacacc ctgctcaccg ccgaccgcgt cacctatcgc
     4801 gagccgaccg acgaggtgct cgccgagggg cacgtggtgc tgagccgcgg tggcgacacg
     4861 atccgcgggc cgaaggccac gctggtggtg ggcgaccgtg tcggcagctt cgagacgccg
     4921 acctacgagc tctcgcgtgc gcgtccgccc gcgctcgccg gcgacgcgcc gcgcaccgtg
     4981 gccggcagcg gcgaggccga tctgctgcgc ctggagggcg agaaccagta ccgcctcgcc
     5041 aacgcgacct ggaccacctg cagcccgcag gacccggact ggtacatcaa ggcgcgcgag
     5101 ctcgagctcg actacgaccg cgaggtcggc accgcccacg ccagctcgat cgtgttcatg
     5161 gacacgccga tcttctggat gccctgggcc gaattcccgc tcagcggcca gcgccagtcg
     5221 gggctgctgc cgcccacctt cggctcgtcg aacaagaccg gggtggactt cacccagccg
     5281 tactactgga atatcgcacc caactacgac gcgaccctgg cgccgcgcat catgacccgg
     5341 cgcggcgtcc agttcggcgg cgagttgcgc tatctcggcg aggactccca gggcaccacg
     5401 cgcctcgagt ggatgccgaa cgacaaggtg accggcgagg agcgcaggct cggctcgatc
     5461 cagcaccagc agcgcttcgc ccccaacttc tacggcacgc tcgatctcaa cgcggtgtcc
     5521 gacgacggct acttcgagga cctgtcctcc aacatcggcg tggcctcgcg ggtcaacctg
     5581 ctgcgcgagg ggcggctgat ctacacgggc ggctggtgga ccgcctccgc gctggcgcag
     5641 cgctaccaga cgctgaaccc ggatccggac gtgcgcaacg tcgagcccta tcgccgcctg
     5701 ccgcagttgc gcctcgacgc cgtgcgcgcc gacctcgccg gcggcatggc cggcctgctc
     5761 gagaccgagt tcgtccgttt cgcccatcgc gagagggatc gcgacgaggc cgcgcgcgtc
     5821 gtcgcctatc cgcagctgtc gttgccgatg cagggcgcgg cctggttcgt cacgcccaag
     5881 gcgggcgtgc attacaccag ctacgacatc gaccgcgtcg cggcgacttc cggcaagccc
     5941 gaatcgatca cgcgctcggt cccgatcgtc tcgctcgact ccggcctgtt cttcgagcgc
     6001 gagacgaact atttcggcag cgactacctg cagacgctcg agccgcggct gttctacctg
     6061 cgcgtgccga gccgcgagca gtcggacatc ccgctcttcg acaccaaccg cttcgacgtc
     6121 ggtttcgcgc agatcttcgc cgagaaccgc tactcgggca gcgaccgcat cggcgacgcc
     6181 aacgacctca ccgcggcgct taccacccgc ttcatcgatg cggagaccgg ggtcgagcgc
     6241 ctgcgcgcgc tgatcggcca gcgctactac ttctccgacc agaaagtcta tctgtacgac
     6301 gaggacgaac tgcgccgcgc cggcaccggc aacgaaatcc tcgccggcct cggcggacgc
     6361 atcaccagca cggtgacggt ggattcctac gcccagtaca acaccgagac cagccgcacc
     6421 gagcgcctca acgcgagcgt gcgctaccag cccggcttcg cgcgcaccct caacctgagc
     6481 taccgctacg cgcccaacct gcgtgtcgat caagacatcg tcggcctcga ggacatcgac
     6541 gtctccggcc agtggccgct cgcgcgccag tggtacggcg tcggtcgagt cacccactcg
     6601 ctcaaggacg accgcatcac cgaggcggtc gcgggtatcg agtacgacgg cggctgctgg
     6661 gtgttccgca ccgcggtgca tcgcttcgcg atcgacgagg acgacgtgac caacgccatc
     6721 ttcatccagc tcgaactgaa cgacctcgcc gggatcggct cgaatccgct cagcctgatc
     6781 aagcgcagcg tccccggcta tggcaagatc aacgactcct cggccaaccg cgtgttcggc
     6841 aacaactgaa gccggttccg cgcttccacc tccaaacaca tgaaacgact ccttttccgc
     6901 tcccgcgtcg caatcgccat cggcctcgcg gccaccacgc tcgcgctgcc ggtgcattcc
     6961 gcgccgcgcg cggtcgaggt cgaccgcatc gtcgcggtcg tcaacaacga ggtcatcacc
     7021 ggcctcgagc tgcgcgcgcg catcgagcag acccgccgcc agctcgcccg ccagggcgcc
     7081 cagcttcccc ccgaggaagt cctgcagcgc cagctgctcg agcgcctgat cgtcgagcgt
     7141 gcccagctcc agctcgcgcg cgagtcctcg ctgcgcgtgg atgacgtcac gctcgaccgc
     7201 gcgatcgagc gcatcgcctc gaacaacaag ctctcgatcg accagttgcg cgccacgctg
     7261 gagaaggatg gcgtcacgtg gagccgcttc cgcgacgaga tccgcagcga gatcctgctc
     7321 acccgcctgc gcgaacgcga ggtcgacagc cgcatcgtcg tcaccgacgc cgagatcgac
     7381 aacttcatcg ccaacaaccc ggacgccttc tccggccagg agttcgccgt cgcccacatc
     7441 ctgctgcgca cgcccgaggg cgcctcgccg cagcaggtcg aggcggtggc caggcgcgcc
     7501 gagcaggtga tggcgcggct gcgctcgggc gaggacttcg cccgcgtcgc cgccgaggtc
     7561 tccgacgcgc ccgacggcct gcagggcggc agcctgggct ggcgcccgct cgatcgcctg
     7621 ccggccctgt tcgccgacgc cgtgcgccgc atgcgcccgg gcgagacttc gccggtgctg
     7681 cgcagcgccg ccggcctgca catcgtgcgc ctggtcgacg cgcgcggcgg cggtgcggcg
     7741 gcggtgcaga agctcgagca gacgcgcgcg cgccacatcc tgatcaagac ctccgaggtc
     7801 ctctccgacg ccgacgcgga ggcccgcctg ctggcgatcc gcgaacgcgt ggtcaatggc
     7861 gccgacttcg ccgagctggc caaggcgagt tcggccgacc tctccgccgc ccgcggcggc
     7921 gatctcggct ggctcaaccc gggcgacacc gtgcccgagt tcgagcgcgc gatgaacgcg
     7981 ctgcggccgg gcgaggtcag cgcgccggtg cgctcgcctt tcggctggca tctgatccag
     8041 ctcgtggagc gccgcatgca ggacgtcacc gacgagcgca agcgcgccgc cgcgcgtcag
     8101 gccctgcgcg aacgcaaggc cgagcaggcc tacgaggact ggttgcgcca gctgcgcgac
     8161 agcacctacg tcgactaccg catcgagcgc gaatgagtgt cgccgcggaa gcggggcgca
     8221 ggctcgtcgc catcaccagc ggagagcccg cgggggtcgg ccccgagctg tgcctgcgcc
     8281 tggccgagcg cgcctggccg ctgcgcccgg tggtgctcgc cgacatcggg ttgatgcgtg
     8341 cgcgagccgt ggcgctcgac agcgctgtgg gcgtggtcga attcgacgcc gcgcggcctg
     8401 cggccgaggg cgcggtcgag gtgctgcatc tgccgctcgc ggcgccttcc cgggccggcg
     8461 tgctcgatcc ggccaacggc gcctacgtgt tgcgcttgct cgaccgcgcg atcgccggct
     8521 gcctgcaggg cgaattcgcc gccatggtca ccgcgccggt gcacaagggc gtgatctgcg
     8581 agggtctcgg cgcccacgag accatgccct tcaccggcca caccgaatac ctcgccgagc
     8641 acaccggcac gccgcgggtg gtgatgatgc tggtgggcgg cggcctgcgc gtagcgctgg
     8701 cgaccaccca cctgccgctc gccgcggtgc cggcggcgat cacgccgcag gtgctcgaag
     8761 agaccctgcg catcctgcac gccgacctgc gccgccactt cggcctcgcc gcgccgcgca
     8821 tcctggtcgc cggcctcaac ccgcatgcgg gcgagggcgg ccacatgggg cgcgaggaga
     8881 tcgacgtcat catcccggtg ctcgagcgcc tgcgcgccga gggcatgcag ctcgtcggtc
     8941 ccttgcccgc cgacaccctg ttcgtgccgc acacgctggc gcagggcgat gcggtgctgg
     9001 cgatgtacca cgaccagggc ctgccggtgc tcaagcacgc cagcttcggc ggcggcgtca
     9061 acgtcacgct cggcctgccg atcatccgca cttccgtaga ccacggcacc gcgctcgacc
     9121 tcgccggcag cggccgcgcc gatccgggca gcctgttcgc cgcgctcgag ctggcgatcg
     9181 cgatgacgca ggcgcgcgcc gccgcctgag cgcggcgcag ccaggccggg ctgccccggc
     9241 gaaaggaaat ccatgcacga cttcgacacc gacggccatc gcgcgcgcaa gcgcttcggc
     9301 cagaacttcc tctccgaccc caacatcatc cgcaagatca tcgacggcat ccgcccccag
     9361 ccgggcgagc tgatggtcga gatcggcccc ggcctggggg cgatgaccga tccgctgatc
     9421 gagcgtctcg gccacctgca cgtggtcgag atcgaccgcg acctcatcgc ccgcctgcac
     9481 gagcgctaca cgccggcgca gctcacggtg cacgagggcg acgcgctgaa gttcgacttc
     9541 ggcagccttt gcgccggcga ggaggggggc gtcgggccca ggctgcgcat cgtcggcaac
     9601 ctgccctaca acatctccac ccccatcctg ttccacctcg ccggctttgc cgaccaggtc
     9661 aaggacatga ccttcatgct gcagaaggag gtggtgatgc gcatggtggc
//
LOCUS       DAOQBS010000115         9702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_6387_length_9702_cov_15.574272, whole genome shotgun sequence.
ACCESSION   DAOQBS010000115 DAOQBS010000000
VERSION     DAOQBS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9702
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6387_length_9702_cov_15.574272"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PKN25_11950"
     CDS             complement(<1..882)
                     /locus_tag="PKN25_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNV91655.1"
                     /translation="MKLYYADHFVLPLPAGHRFPMEKYSRLRARLRDSGLFADDDLRV
                     PAAASDAEILRAHDAGYLQRVAGGTLDAAEVRRIGFPWSAAMVERSRRSAGATLAACR
                     SALDREGGAACAANLAGGTHHAHRDFGSGFCVFNDAAIAALAMRAEGRAGRIAIVDCD
                     VHQGDGTATILADTPEVFTCSLHGAKNFPFRKQASDLDVELPDDTGDEAYLAALDAAL
                     DTVFTRARPELVIYLAGADPYAGDRLGRLALSIDGLRRRDERVLGRCRAADVPVAVAM
                     AGGYAQPIDDTVTIHFNT"
     gene            954..2252
                     /locus_tag="PKN25_11955"
     CDS             954..2252
                     /locus_tag="PKN25_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNV91656.1"
                     /translation="MRRNWCAKHKPPRSRGGAIDARLERVSRTRTAAAGRGRESAPSR
                     GGQDAGGHEPDALQRGWIVLRRGAFLLSGVYGAVQYVECRFHVHSKSRLKLGFRLFLA
                     SIRAAILRFCAGKTAARARLPGTRVASTAVPPVPADAPEVAPASISEPGPAHAESPSM
                     PERRDPLGAESAPDEHVEPAAIVAAPDPDAERLLARCARLQAQMLDFTGRLADMEQQV
                     RSYEQQQFLALGDVVGECLRLRREYLDLKAERSGAAADREQARQAEADFDAYRRSTAH
                     TPAPRPELDDDEQDELRRLYRAAAMRCHPDRAEEAERAEAHDVFLRVQAAYQARDLAG
                     LRAITAELAGWARKRDDASPVQGSAAPASSAVRRRVAELQTRVADLILAVQTLQLDPA
                     YRRAVQVERWETHFAEARAGFEAECESLREQIAGLASRRS"
     gene            complement(2308..3771)
                     /gene="dbpA"
                     /locus_tag="PKN25_11960"
     CDS             complement(2308..3771)
                     /gene="dbpA"
                     /locus_tag="PKN25_11960"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HNV91657.1"
                     /translation="MTEPTPPSSPILASPPDAPPGSLPPATPGLPFDGLPLSPAMLDT
                     LRQLDYRAMTPIQAASLPLALAGHDLIAQAKTGSGKTAAFALPLLARLDVRKLAVQAM
                     VLCPTRELADQVTQEIRRLARFEANIKVLALCGGTPMRPQRDSLEHGAHVVVGTPGRI
                     MDHLERGSLALEALNTLVLDEADRMLDMGFHDDIAFVASRCPSERQTLLFSATYPEGI
                     ARLAARFLREPQEVKLLEQHAHGKIRQRFYEVAHPARLEAVATLLRHFRPVSTLAFCN
                     TRQQCRDLVELLRARGFAALALNGEMEQRERDQVLIQFANRSCSVLVATDVAARGLDI
                     ARLEAVINVDVTPDPEIHIHRIGRTGRGDEEGWALNLCSSADKRRVASIAQRMGFEPE
                     WHALDELVEDANEPLQAPMTTLLILGGRKDKIRPADVLGALTGEAGFTREQVGKITVT
                     DQTTYVAVERAIAREAVRRLSAGTLKGKKVKVRALEV"
     assembly_gap    3836..3935
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4110..5282
                     /locus_tag="PKN25_11965"
     CDS             4110..5282
                     /locus_tag="PKN25_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HNV91658.1"
                     /translation="MSLIESVRPLQSKLTAIRRDIHAHPELAFEERRTAELVARHLEA
                     LGIETHRGIGGTGVVGVVRGRRGLRAIGLRADMDALPITERNEFAHKSTIQGCMHACG
                     HDGHTTMLLGAAEALAARRDFDGTVYLIFQPAEEGEGGAPAMIADGLFERFPMEAVFG
                     MHNWPGMEAGSFAVHAGPVMASADRFDIRFTGVGAHAAMPHLGVDPVVAGAAFVQAAQ
                     TLVSRVLDPIDAGVVSVTQFHAGEAYNVIPDRAELCGTVRTFSAEVRDRLERGLRQVA
                     EGIAQSHGVQVDFEFRRGYPPTVNTVAEATLCAEAARAVAGPERVFTDRRPSMGAEDF
                     AYFLQEKPGAYVWVGNGPGEGGCMLHNPNYDFNDEVLPAGVAYWCALVQRLLGGVK"
     gene            5293..>7105
                     /locus_tag="PKN25_11970"
     CDS             5293..>7105
                     /locus_tag="PKN25_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNV91659.1"
                     /translation="MMRTLRLALRMMLRDLRAGELHLLGLAIMIAVASLTSVGFLADR
                     VGRALDREANQLLGGDLLLRADKPWPDAFIDEARSRGLQTARTVLFTSMASTAEGAAL
                     GGVKVVEDGYPLRGTIRLAPGPNQADAPAGRAPEAGEVWLDERLFAELGVNTGDKVGL
                     GELEFRVGAMVSFEADRGANFFSLLPRAIFNLKDLEATGLIAEGSRATWRLHVAGTPQ
                     AVEAYEKWARANLGRGQRVETVDNARPEVRAALDQAQRFLRLAALLAVILAAVAVGLS
                     ARRFMARHLDGCAVMRVLGARQAQVLGIVVGEFLLFGLAAALAGTALGWAVQWGLAGG
                     LRELLATDLPAPSPLPLAHGLVVGMALLAGFVLPQLLRLGKVSTLRVLRREFEFAEPV
                     SGAAWALGLAALLGLIFWIAADARLGAMVAAGFAVALGVFALAAWGVLRLAGRLKGQG
                     SLRGGGWRYGIAALGRRMGSSVIQSAALGLGMTALLLLTLVRADLLDNWRRMAPPDAP
                     NRFVINIQPDQRAGIAARFAAEGLPVPPIQPMIRGRMVAIEGKPVDPSAYEDERTRRL
                     AEREFNLSYGAELPPGNVVEAGRWHGAAAQPQFSAQFS"
     assembly_gap    7106..7205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7206..7927
                     /locus_tag="PKN25_11975"
     CDS             <7206..7927
                     /locus_tag="PKN25_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNV91660.1"
                     /translation="QFSAQFSVEAGLAKTFGLAVGDRVTFEIGGRRVEAPITSVRKLD
                     WDSMRVNFFFIASPGVLEDFPATLITSFHLPPARHDFTTKLVGEFPNLTVIDTAAVIA
                     QVQSMTDKLIVIVQFVFGFAVLAGLVVLYAALQSTHDERDYELAMLRTLGARNRQVRQ
                     ALFAEFLVLGGVAGVLAGVGASAIGWVLAEQVFRMPYVPALAPVLVGVVLGAAGVVAG
                     GWLGTRALLSRPPLASLRALG"
     gene            7932..9185
                     /locus_tag="PKN25_11980"
     CDS             7932..9185
                     /locus_tag="PKN25_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNV91661.1"
                     /translation="MTSLQLTPAEWLLAGLLVAVLVLQAWGLLRGGGAERRLREGLGD
                     DLTDFMEQRLAARHRELLLDLHDGLARQTDRIGEHARADRELLQRGLTAASLQLSRGM
                     QALTQSVDGRLDTLSGQVNQRLDEGFRKTNETFASVMARLATIDEAQKKIDGLTTNVV
                     SLQELLGDKRARGAFGEVQLENLVRNMLPPEAFAFQQVLPNGTRVDCLLTLPAPTGNV
                     AVDAKFPLENYHRMFDDTLAELDRRAAQQAFRADVKRHVDAISAKYILPGSTADGAVM
                     FLPAEAVFAELHAYHPEIVAYAQQKRVWIVSPTTLMAVLNTARAVMKDVETRKQIHVI
                     QDALGKLAKDFQRFDERMAALARHIEQASRDVQDVHTSSRKISAHFRRIESVQLDEED
                     EEAGEGTPAALAAGTVGSGSVGGRD"
     gene            9377..9661
                     /locus_tag="PKN25_11985"
     CDS             9377..9661
                     /locus_tag="PKN25_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91662.1"
                     /translation="MSDFPLPILFIMAVFFTMWLILRKFHRIEQRKRALPELNAYLYA
                     NNQTEPRCSACESTEFKDEGLSHGKDERRIVSCAKCDTLLYRIVPPERAE"
BASE COUNT         1293 a         3319 c         3481 g         1409 t
ORIGIN      
        1 ggtgttgaag tggatcgtca ccgtgtcgtc gatcggctgc gcgtagccgc cggccatggc
       61 cacggcgacc ggcacgtcgg cggcgcggca ccggccgagc acgcgttcgt cgcgccggcg
      121 caggccgtcg atgctgagcg cgagccggcc gaggcggtcg ccggcgtagg ggtcggcgcc
      181 ggcgaggtag atcaccagct cggggcgggc gcgggtgaag acggtgtcga gcgccgcatc
      241 gagcgcggcg aggtaggcct cgtcgccggt gtcgtcgggc agttcgacgt cgaggtcgct
      301 cgcctgcttg cggaagggga agttcttcgc accgtgcagg ctgcaggtga agacctcggg
      361 cgtgtcggcg aggatggtcg cggtgccgtc gccctggtgc acgtcgcaat cgacgatggc
      421 gatgcggccg gcgcggccct cggcccgcat cgccagcgcg gcgatggcgg cgtcgttgaa
      481 cacgcagaag cccgagccga agtcgcgatg tgcgtggtgc gtgccgccgg cgaggttggc
      541 ggcgcaggcc gcgccgccct cgcgatcgag cgcgctgcgg caggcggcca gcgtggcgcc
      601 cgccgagcgg cgcgagcgct cgaccatcgc cgcgctccat ggaaagccga tgcggcggac
      661 ctcggccgcg tccagcgtgc cgccggcgac gcgctgcagg tagccggcgt cgtgggcgcg
      721 caggatctcg gcgtcgctgg cggcggcagg gacgcgcaag tcgtcgtcgg cgaacaggcc
      781 gctgtcgcgc aggcgcgcgc gcagccgcga gtatttttcc atcggaaagc ggtggcccgc
      841 gggcagcggg aggacgaaat ggtcggcgta gtagagcttc aaggtggacg ggttccggtt
      901 gaactcgggc gcggcaaggg cgggcgttct gttcccgcac ggcttgggac cgtgtgcgcc
      961 gcaattggtg cgcaaagcat aagccgccgc gcagccgcgg cggcgccatc gacgcccgcc
     1021 ttgaacgcgt atctcgaacg cgcacggccg cagctgggcg gggacgtgaa tccgcgcctt
     1081 cgaggggcgg tcaggatgct ggcggacacg agccggacgc gctgcaacgg gggtggattg
     1141 tcctgcgccg tggagcgttc ctgttgtcag gcgtttatgg tgcagtgcaa tatgtcgaat
     1201 gtcgattcca tgtccactcc aaatcgaggc tgaaattggg tttccgcctg ttcctggcaa
     1261 gcatccgtgc ggccatcctg cgcttctgcg ccggcaagac cgccgcccgc gcgcgtctgc
     1321 cggggacgcg cgtcgcctcc acggccgtgc cgccagtgcc ggcagatgcg ccggaagttg
     1381 cacccgcgtc gatatccgag cctgggcccg cacacgcgga gtccccgtcg atgcctgagc
     1441 gtcgcgatcc actcggcgcc gaatccgcgc ccgacgagca tgtcgagcct gccgccatcg
     1501 tcgccgcgcc cgatccggac gccgaacgat tgctcgcgcg ctgtgcccgt ctgcaggccc
     1561 agatgctcga cttcaccggc cgtctcgccg acatggagca gcaggtgcgc agctacgagc
     1621 agcagcagtt ccttgccctc ggcgacgtgg tgggtgaatg cctgcgcctg cgccgggaat
     1681 acctcgacct gaaggccgag cgctcgggtg cggcggccga tcgggagcag gcgcgccagg
     1741 ccgaagcgga cttcgacgcc taccgccgca gcaccgcgca cacgcccgcg ccgcggcccg
     1801 aactcgatga cgacgagcag gacgaactgc gccggctgta tcgcgcggcg gccatgcgct
     1861 gccatccgga ccgtgcggag gaggccgagc gcgccgaggc gcacgacgtc ttcctgcgcg
     1921 tgcaggctgc ctaccaggcg cgcgacctcg cggggctgcg cgccatcacc gccgagctgg
     1981 cggggtgggc gcgcaagcgc gacgacgcga gccctgtgca ggggagcgcg gcgcccgcga
     2041 gcagcgccgt ccggcgtcgg gttgccgaac tgcagacccg ggtcgccgac ctgatccttg
     2101 ccgtgcagac cctgcaactg gatcccgcgt accgccgggc cgttcaggtc gagcgctggg
     2161 aaacgcactt tgccgaagcg cgcgccggct tcgaggccga atgcgagagc ttgcgcgagc
     2221 agatcgccgg actggcgtcg cgtcgaagtt gagaccggcg cggggcgccc gcggctcaaa
     2281 gcacccaccg cgagcctcgc ccccgcatca cacttccagc gcgcgcacct tcaccttctt
     2341 gcccttgagc gtgcccgccg acagccggcg caccgcctcg cgcgcgatcg cgcgctcgac
     2401 cgcgacgtag gtggtctggt cggtgacggt gatcttgccg acctgctcgc gggtgaagcc
     2461 ggcctcgccg gtcagcgcgc cgagcacgtc cgcagggcgg atcttgtcct tgcgtccgcc
     2521 caggatcagc agcgtcgtca tcggcgcctg caggggctcg ttcgcgtctt ccaccagttc
     2581 gtcgagggcg tgccactcgg gctcgaagcc catcctctgc gcgatgctcg cgacgcggcg
     2641 cttgtccgcg ctgctgcaca ggttcagcgc ccagccctct tcatcaccgc ggccggtgcg
     2701 gccgatgcgg tggatgtgga tctccgggtc gggggtgacg tcgacgttga tcaccgcctc
     2761 gagacgtgcg atgtccagcc cgcgcgcggc gacgtcggtg gcgacgagca ccgagcagct
     2821 gcggttggcg aactggatca gcacctggtc acgctcgcgc tgctccatct cgccgttgag
     2881 ggcgagcgcc gcgaagcccc gcgcgcgcag cagctcgacg aggtcgcggc actgctggcg
     2941 ggtgttgcag aaggccagcg tgctgaccgg gcggaagtgg cgcagcagcg tcgcgaccgc
     3001 ctccagccgc gccgggtggg cgacctcgta gaaacgctgg cggatcttgc cgtgcgcgtg
     3061 ttgctcgagc agcttcacct cctgcggctc gcgcaggaaa cgcgcggcca ggcgggcgat
     3121 gccctcgggg taggtggcgg agaacagcag ggtctggcgc tcggaggggc agcggctggc
     3181 cacgaaggcg atgtcgtcgt ggaaacccat gtccagcatg cggtcggctt cgtccagcac
     3241 cagcgtgttc agggcctcga gcgccaggct gccgcgttcg aggtggtcca tgatgcgccc
     3301 gggcgtgccg accacgacgt gggcgccgtg ctcgaggctg tcgcgctgcg ggcgcatcgg
     3361 cgtgccgccg cacagcgcca gcaccttgat gttggcctcg aagcgcgcca ggcggcggat
     3421 ctcctgggtg acctggtcgg ccagctcgcg ggtcgggcac agcaccatgg cctggacggc
     3481 gagcttgcgc acgtcgagcc gggccagcag gggcagggcg aaggccgcgg tcttgccgct
     3541 gccggtcttg gcctgggcga tgaggtcgtg cccggcgagg gccagcggca ggctggccgc
     3601 ctggatcggg gtcatggccc ggtagtcgag ctggcgcagg gtgtccagca tggcgggcga
     3661 gagcggcagg ccgtcgaaag gcaggccggg ggttgcgggc gggagtgacc cgggcggggc
     3721 gtccggggga ctcgcgagga tgggggagga ggggggcgtc ggttcggtca tgcgcgatta
     3781 tcgcggcgga ctcgacatct tccctagaga actttgtgcg gtcggccgga atcgcnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgcgg tcgagacgcc acgacgcctg
     3961 gaatgcatgc cgaccatcgt gatcccgcgt cgcgccagcc gtctcccttg ctctcgcccc
     4021 aaccggattg aggacgccga aggatccacc cgcgtccgcc tgtactacac tcggacaccc
     4081 gcgacaaacc acgacaacac ggtgcagcca tgagcctcat cgaaagcgtc cgccccctgc
     4141 agagcaagct gaccgcgatc cgccgcgaca tccacgccca ccccgagctc gccttcgagg
     4201 agcgccgcac cgccgagctg gtggcgcgcc atctggaggc gctcggcatc gagacccatc
     4261 gcggcatcgg cggcaccggc gtggtcggtg tggtgcgcgg ccgccgcggg ctgcgtgcga
     4321 tcgggctgcg cgccgacatg gatgcgttgc cgatcacaga gcgcaacgag ttcgcgcaca
     4381 agtccaccat ccagggctgc atgcacgcct gcggtcacga cggtcacacc accatgctgc
     4441 tcggcgcggc cgaggcgctg gccgcgcggc gcgacttcga cggcacggtg tacctgatct
     4501 tccagcccgc cgaggaaggc gagggcggcg cgccggcgat gatcgccgac ggactcttcg
     4561 agcgctttcc catggaggcg gtgttcggca tgcacaactg gccgggcatg gaggcgggga
     4621 gcttcgccgt acacgccggc ccggtgatgg cgagcgccga ccgcttcgac atccgcttca
     4681 ccggcgtcgg cgcgcatgcg gcgatgccgc acctcggcgt ggacccggtg gtggcaggcg
     4741 cggccttcgt gcaggcggcg cagaccctgg tgagccgcgt gctcgatccg atcgacgccg
     4801 gcgtggtgtc ggtgacccag tttcatgccg gcgaggccta caacgtgatc cccgaccgcg
     4861 ccgagctgtg cggcacggtg cgcaccttct ccgccgaggt gcgcgaccgc ctcgagcgcg
     4921 gcctgcgcca ggtggccgag gggatcgcgc agagccatgg tgtgcaggtg gatttcgagt
     4981 tccgccgcgg ctatcccccg accgtcaaca ctgtcgccga ggccacgctg tgcgccgagg
     5041 cggcgcgcgc ggtggcggga ccggagcggg tattcaccga ccgccgcccg agcatgggtg
     5101 cggaggattt cgcttatttc ctgcaggaga agccgggcgc ctacgtgtgg gtcggcaacg
     5161 gcccgggcga gggcggctgc atgctgcaca accccaacta cgacttcaac gacgaggtgc
     5221 tgccggccgg cgtggcctac tggtgcgcgc tggtgcagcg cctgctcggc ggcgtgaagt
     5281 gagcgccgcc cgatgatgcg cacgcttcga ctcgccctgc gcatgatgct gcgcgacttg
     5341 cgcgcgggcg agctgcacct gctcgggctg gcgatcatga tcgcggtggc gagcctgacc
     5401 agcgtgggct tcctcgccga ccgtgtcggg cgcgcgctcg atcgcgaggc caaccagctc
     5461 ctcggtggcg acctgctgct gcgcgccgac aagccgtggc ccgacgcctt catcgacgag
     5521 gcgcgcagcc gcggcctgca gaccgccagg acggtgctgt tcaccagcat ggcgagtacc
     5581 gccgagggcg cggcgctcgg cggcgtcaag gtggtggagg acggctaccc gctgcgcggc
     5641 accatccgtc tggcgcccgg tcccaaccag gccgacgccc ccgcaggtcg cgcgcccgaa
     5701 gcgggcgagg tgtggctgga cgagcgcctg ttcgccgagc tgggcgtgaa caccggcgac
     5761 aaggtgggcc tcggcgagct cgagttccgt gtcggcgcca tggtcagctt cgaggccgac
     5821 cgcggcgcca acttcttcag cctgctgccg cgcgcgatct tcaacctcaa ggatctggag
     5881 gccaccggcc tgatcgccga gggcagccgc gccacctggc gcttgcacgt ggccggcacg
     5941 ccgcaggcgg tggaggctta cgagaagtgg gcgcgcgcga acctcggccg cggccagcgc
     6001 gtcgagaccg tggacaacgc ccgccccgag gtgcgtgccg cgctcgacca ggcgcagcgc
     6061 ttcctgcgcc tggcggcgct gctcgcggtg atcctcgccg cggtggcggt gggcctgtcc
     6121 gcgcgccgct tcatggcacg ccacctcgac ggttgcgcgg tgatgcgcgt gctcggtgcg
     6181 cgtcaggcgc aggtgctggg cattgtggtg ggcgagttcc tgctcttcgg gctggccgcc
     6241 gcactcgccg gtaccgcgct gggctgggcg gtgcagtggg ggctggcggg cgggctgcgc
     6301 gagctgctcg ccaccgactt gcccgcgccc tcgccgctgc cgctcgcgca tggcctggtg
     6361 gtgggcatgg cgctgctcgc cggcttcgtg ctgccgcaac tgctgcgcct gggcaaggtc
     6421 agcaccctgc gcgtgctgcg ccgcgagttc gagttcgccg agccggtctc gggtgccgcc
     6481 tgggcgctcg ggctggcggc gctgctcggg ctgatcttct ggatcgccgc cgacgcgcgc
     6541 ctcggcgcca tggtggcggc cggcttcgcg gtggcgctcg gcgtgttcgc gcttgccgcc
     6601 tggggtgtgc tcaggctcgc cggccgcctc aagggacagg gcagcctgcg cggtggcggc
     6661 tggcgctacg gcatcgccgc cctcggccgc cgcatgggca gcagcgtgat ccagtccgcc
     6721 gcgctcgggc tgggcatgac cgcgctgctg ctgctcaccc tggtgcgcgc cgacctgctc
     6781 gacaactggc ggcgcatggc gccgccggac gcgcccaacc gcttcgtgat caacatccag
     6841 cccgaccagc gcgccggcat cgccgcgcgg ttcgccgccg agggcctgcc ggtgccgccc
     6901 atccagccga tgattcgcgg ccgcatggtg gcgatcgagg gcaagccggt cgacccctcg
     6961 gcgtacgagg acgagcgcac gcggcgcctg gccgagcgcg agttcaatct gtcgtacggc
     7021 gccgagttgc cgccgggcaa cgtcgtcgag gcggggcgct ggcacggggc ggcggcgcag
     7081 ccgcagtttt ccgcgcagtt ttccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnncgcag ttttccgcgc agttttccgt cgaggccggg ctggcgaaga ccttcgggct
     7261 ggcggtgggc gaccgtgtga cgttcgagat cggcggccgc cgcgtcgagg cgccgatcac
     7321 cagcgtgcgc aagctcgact gggactcgat gcgggtgaac ttcttcttca tcgcctcgcc
     7381 cggcgtgctg gaggatttcc ccgcgaccct gatcaccagt ttccacctgc cgcccgcgcg
     7441 ccacgacttc accacgaagc tggtcggcga gttccccaac ctcaccgtga tcgacaccgc
     7501 ggcggtgatc gcacaggtac agtcgatgac cgacaagctg atcgtgatcg tgcagttcgt
     7561 gttcggcttc gcggtgctcg ccgggctggt cgtgctgtat gccgcgctgc agtccactca
     7621 cgacgagcgc gactacgagc tcgccatgct gcgtaccctg ggcgcgcgca accgccaggt
     7681 gcgccaggcc ttgttcgccg agttcctggt gctgggtggg gtcgccggcg tgctcgcggg
     7741 cgtgggcgcg agcgcgatcg gctgggtgct ggccgagcag gtcttccgca tgccctacgt
     7801 gccggcgctg gcgccggtgc tggtgggcgt ggtgctgggc gcggccggcg tggttgccgg
     7861 tggctggctg ggcacgcgtg cgctgctgag ccggccgccg ctcgccagcc tgcgtgcact
     7921 gggctgaacg catgacgtcc ttgcaactga cgccggccga gtggctgctc gccggcctgc
     7981 tggtggcggt gctggtgctg caggcctggg ggctgctgcg cggcggcggc gccgagcgcc
     8041 gcctgcgcga aggcctcggc gacgacctca ccgacttcat ggagcagcgc ctggccgcgc
     8101 gccaccgcga gctgctgctc gacctgcacg acgggctcgc gcgccagacc gaccgcatcg
     8161 gcgagcacgc gcgcgccgac cgcgagctgc tgcagcgcgg cctcaccgcc gcctcgctgc
     8221 agctgtcgcg cggcatgcag gcgctcacgc agagcgtgga cggccgtctc gacaccctct
     8281 cgggtcaggt caaccagcgc ctcgacgagg gcttccgcaa gaccaacgag accttcgcca
     8341 gcgtgatggc gcggctggcg acgatcgacg aggcgcagaa gaagatcgac ggcctcacca
     8401 ccaacgtggt cagcctgcag gagctgctcg gcgacaagcg cgcacgcggc gccttcggcg
     8461 aggtccagct cgagaacctg gtgcgcaaca tgctgccgcc cgaggccttc gccttccagc
     8521 aggtgctgcc caacggcacc cgggtggact gcctgctgac cctgccggcg cccaccggca
     8581 acgtcgcggt cgatgccaag ttcccgctcg agaactacca ccgcatgttc gacgacacgc
     8641 tcgccgagct cgaccgccgc gccgcgcagc aggccttccg cgccgacgtg aagcgccacg
     8701 tggacgcgat ctccgccaag tacatcctgc ccggcagcac cgccgatggc gcggtgatgt
     8761 tcctgcccgc cgaggcggtg ttcgccgagc tgcacgccta ccacccggag atcgtggcct
     8821 acgcgcagca gaaacgcgtg tggatcgtgt ccccgaccac gctgatggcg gtgctcaaca
     8881 ccgcgcgcgc ggtgatgaag gacgtcgaga cgcgcaagca gatccacgtc atccaggacg
     8941 ccctcggcaa gctcgcgaag gacttccagc gcttcgacga gcgcatggcc gcgctcgccc
     9001 gccacatcga gcaggccagt cgcgacgtgc aggacgtgca cacctcgagc cgcaagatca
     9061 gcgcgcactt ccgccgcatc gagtcggtgc agctggacga ggaggacgaa gaggcgggcg
     9121 agggcacgcc ggcggcgctc gctgccggga ccgtgggaag cggatccgtg ggggggcggg
     9181 attgagttct gcgaacctgg ggaattcttc cggggccgct gcggtcggac cacccgtcac
     9241 gggcgcgcac ggatgatcgt ccgcccgatt tgatgcggga cagggacggg gcgcgccgcc
     9301 gcccgtatcg tcaggcccga ctcgccgcaa ggcctgcatc gcttctccga ttcacccgtc
     9361 cgggaacccc gcaccgatgt ccgattttcc cctgccgatc cttttcatca tggcggtctt
     9421 cttcacgatg tggctcatcc tgaggaagtt ccaccgcatc gagcagcgca agcgcgcgct
     9481 ccccgagctc aacgcctact tgtacgccaa caaccagacc gaaccgcgct gctcggcctg
     9541 cgaatcgacc gaattcaagg acgagggcct cagccacggc aaggacgagc gtcgcatcgt
     9601 gtcgtgcgcc aagtgcgaca ccctgctgta ccgcatcgtg ccgccggaaa gggcggagta
     9661 gtcgctccgc caatcgcgac ttgcgtcgct cccacagccc tg
//
LOCUS       DAOQBS010000116         9502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_6593_length_9502_cov_15.522176, whole genome shotgun sequence.
ACCESSION   DAOQBS010000116 DAOQBS010000000
VERSION     DAOQBS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9502
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6593_length_9502_cov_15.522176"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2396
                     /gene="gyrA"
                     /locus_tag="PKN25_11990"
     CDS             <1..2396
                     /gene="gyrA"
                     /locus_tag="PKN25_11990"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNV91663.1"
                     /translation="DTIVRMAQDFSLRYMLVDGQGNFGSVDGDNAAAMRYTEIRMARI
                     GHELLADIDKETVDFGPNYDGSEKEPLVLPARIPNLLINGSAGIAVGMATNIPPHNLG
                     EIVDACLKLLAEPDTDIEALIDIVQAPDFPTAGLIYGLAGVREGYRTGRGRVIMRART
                     HFEPIGKTDRQAIIVDELPYQVNKRTLQERMAELVNEKKIEGISEIRDESDKSGMRLV
                     IELKRGEMPEVVLNKLFKHTQLQDSFGMNMVALVDGKPRLLNLKQMLVCFLEHRREVV
                     TRRTIFELRKARDRGHILEGLAVALSNVDEIIALIKAAPTPADAKRELMAREWRSALV
                     EEMLSRALADSYRPEGLDPQYGLQAQGYRLSEAQAQAILELRLQRLTGLEQDKIVGEY
                     REVMDVITDLLDILARPERITAIIVEELGAIRNQFGDPRRSELVMNTAEINIEDLITP
                     EDMVVTLSHTGYFKRQPLADYRAQRRGGRGKQATSMKDEDFIDHLFVANTHDTVLCFS
                     NRGRCYWLKVYEVPEGTRNSRGKPIVNLFPLVEGEKITAVLPIKEFDEEHFVFMATSE
                     GTVKKTALTAFSNPRKAGIIAVNLDDGDHLIGVAITDGQCDVMLFSDAGKAVRFAETD
                     VRPMGRDARGVRGMTLEDGQAVIAMLVAKDESQSVLTATENGYGKRTPVAEYTRHGRG
                     TKGMIAIQTSDRNGRLVGAVLVEPSDEVMLISTGGVLIRTSVESIREMGRSTQGVTLI
                     NLDEGTFLASIEKVAESESDESMRASGEGAEGGEAEGGAQAAPGEGEAPAADEEGEA"
     gene            2396..3493
                     /gene="serC"
                     /locus_tag="PKN25_11995"
     CDS             2396..3493
                     /gene="serC"
                     /locus_tag="PKN25_11995"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNV91664.1"
                     /translation="MSRVWNFSAGPAVLPEEVLRQAADEMLDWHGSGMGVMEMSHRGK
                     EFTAIAAQAEADLRELLAVPAGYRVLFMQGGAIAENAIIPMNLMGDRRVADYVVTGSW
                     SQKSQKEARKYAEVNIAASSEAGGFTTVPAMADWRLSADPAYVFTCTNETIGGLEYPF
                     EPDLAQIGRGEVPVVADMSSHILSRVIDVSKYGLIFGGAQKNIGPAGLTIVIVREDLL
                     GRAMPHCPTAFDFKTVADNGSMYNTPPTYGIYIAGLVFQWLKRQGGVAAIEAQNVAKA
                     KLLYDFLDASDFYENRIDPACRSRMNVPFFLKDETLNDAFLAEAKAAGLVQLKGHKSV
                     GGMRASIYNAMPLAGVQALVDFMRDFAARRG"
     gene            3573..4640
                     /gene="pheA"
                     /locus_tag="PKN25_12000"
     CDS             3573..4640
                     /gene="pheA"
                     /locus_tag="PKN25_12000"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNV91665.1"
                     /translation="MSDELLKLRNEIDRIDEEILARLAERARCAQRVGEIKRGVMYYR
                     PEREAQVLRRLAELNPGPLSSDAVKTIFREVMSACLGLEQPLRVAYLGPAGTFSESAS
                     RKHFGSAPNFLPMAAIDDVFRAVEAGNADYGVVPVENSTEGAVGGTLDLLLANPLKVC
                     GEVRLRIHQQLMSRAEGIGAARRIYSHAQSLAQCHEWLNRNLPHLPRIPVASNAEAAR
                     MASEDPESCAIAGDAAAQLYGLNILAPNIEDDPNNTTRFLVIADHDAGPSGKDRTSLV
                     FSAPNRPGAIHSLLEPMARHGVDMTKLQSRPARSGLWEYVFYADINGHREDPEVAAAL
                     RELDERAAFVKIIGSYPVAAI"
     gene            4703..5800
                     /gene="hisC"
                     /locus_tag="PKN25_12005"
     CDS             4703..5800
                     /gene="hisC"
                     /locus_tag="PKN25_12005"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNV91666.1"
                     /translation="MSIASLAPDYIRAIMAYQPGKPISELAREMGIPEESIVKLASNE
                     NPLGMSARARDAAIAAIGEVSRYPDGGAFALKKALCERFGVKPEQLVIGNGSNDILEL
                     ASQAFLAPGLSAVYSRHAFAVYPLATNARGARGIEVAAKNFGHDLDAMAAAIEPQTRV
                     VFIANPNNPTGTFVPGAELEAFLAKVPRHVLVVLDEAYTEYLAPEQRYDSIAWLARFP
                     NLLVSRTFSKAYGLAGLRVGYGIAHPEVADLMNRVRQPFNVSSVALAAAEAALGDDEF
                     LARSAELNRRGMTQLVAAFRELGLEWIPSAGNFVTFKVGDAIGVNQALLRQGVIVRPI
                     AAYGMPHWLRVSIGLPEENARFIEALRQALA"
     gene            5815..6702
                     /locus_tag="PKN25_12010"
     CDS             5815..6702
                     /locus_tag="PKN25_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNV91667.1"
                     /translation="MAHIGKLVVCGVGLIGGSFALALRRAGAVERIVGIGRRREVLER
                     ACALGVIDEIAEGWADALDGADLVLLAAPVGQTDAILAAMAPHLRPGTIVTDAGSTKR
                     DVVAALRTHLGGALADVVPAHPIAGAEKSGVEAAFAELYMGRKVVLTPLPESRPEAVQ
                     KVRDAWEACGANVVGMTPQEHDRVFAAVSHLPHLLAFGLVDDLAGRSNAPLLFSHAAS
                     GFRDFTRIAGSHPEMWRDICVANRVALLEELDAYLGELARLRMMLVEGDGDGLEAVFE
                     RARRARNAWAEGLPIRTAE"
     gene            6754..8715
                     /locus_tag="PKN25_12015"
     CDS             6754..8715
                     /locus_tag="PKN25_12015"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HNV91668.1"
                     /translation="MEFLDLPPMLGAAGSVRLPGSKSISNRVLLLAALAEGETDIRDL
                     LLSDDVERMLEALRALGVDWRREGDSLDYRVCGVGGPFPVKTGDLFLGNAGTAFRPLT
                     AALALSGGEYRLSGVPRMHERPIGDLVDALRQLGADITCTANEGYPPLHLKPATIRPG
                     GVVRVRGDVSSQFLTALLMALPLTGVETTIEVVGELISKPYIRITLELMARFGVQVGQ
                     QGWERFVVPGGARYRSPGTVFVEGDASSASYFLAAGAIGGGPVRVEGVGRTSIQGDVR
                     FAEALEQLGARITLGDNWIEAAAPAGGVLKAFDLDLNHIPDAAMTLAVAALFADGPCR
                     LRNIASWRVKETDRIAAMATELRKLGAEVEEGADYLVVQRPPRLQPAAIDTYDDHRMA
                     MCFSLASLGGCRVRINDPKCVNKTFPGYFEAFAQVARPVPVLAIDGPSASGKGTVAAR
                     VAEALGWHYLDSGSLYRLVALAAMRAGVAFDDEAGVAALAAGLPARFEGGRVLLGEGA
                     DRDVTDEIRSEACSVGASKVAVLPAVRAALLDRQRDYRAAPGLVAEGRDMGSVIFPDA
                     GLKVFLTASAEARAERRHKQLIEKGLAANMESLLKDLQERDARDAARPVAPLVKLPDA
                     ALLDTTQRDVDEAVAFVLDLVGRGGKSSG"
     gene            8806..>9502
                     /locus_tag="PKN25_12020"
     CDS             8806..>9502
                     /locus_tag="PKN25_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNV91669.1"
                     /translation="MSNATPAFEESFAALFEESLALQEMRAGEVITAEVVRIDQNFVV
                     VNAGLKSESYVPIEEFRNDRGELEANIGDFVHVAIDALEDGYGETRLSRDKAKRIAAW
                     NDLEKALNEGTLVKGVITGRVKGGLTVMTNSIRAFLPGSLVDMRPVKDTTPYEGKEYE
                     FKVIKLDRKRNNVVVSRRAVLEESMGEEREKLLANLKEGTVIKGVVKNITDYGAFVDL
                     GGIDGLLHITDLAW"
BASE COUNT         1526 a         3342 c         3259 g         1375 t
ORIGIN      
        1 acgacaccat cgtgcgcatg gcgcaggact tctcgctgcg ctacatgctg gtcgatggcc
       61 agggcaactt cggctcggtc gacggcgaca acgcggcggc aatgcgatac accgagatcc
      121 gcatggcgcg catcggccat gagctgctgg ccgacatcga caaggagacg gtggacttcg
      181 ggccgaacta cgacggctcg gagaaggagc cgctggtcct gcccgcgcgc atccccaacc
      241 tgctgatcaa cggctcggcc ggcatcgcgg tgggcatggc gaccaacatc ccgccgcaca
      301 acctgggcga gatcgtcgac gcctgcctga agctgctcgc cgagcccgac accgacatcg
      361 aggccctgat cgacatcgtg caggcgcccg acttccccac cgccggcctg atctacggcc
      421 tggccggcgt gcgcgagggc taccgcaccg gccgcggccg cgtgatcatg cgcgcgcgca
      481 cgcacttcga gccgatcggc aagaccgatc gccaggccat catcgtcgac gagctgccct
      541 accaggtgaa caagcgcacc ctgcaggagc gcatggccga gctggtcaac gagaagaaga
      601 tcgagggcat cagcgagatc cgcgacgagt cggacaagtc gggcatgcgc ctggtgatcg
      661 agctcaagcg cggcgagatg cccgaggtgg tgttgaacaa gctgttcaag cacacccagc
      721 tgcaggacag cttcggcatg aacatggtgg cgctggtcga cggcaagccg cgcctgctca
      781 acctcaagca gatgctggtg tgcttcctcg agcaccgccg cgaggtcgtt acccgccgca
      841 ccatcttcga gctgcgcaag gcgcgcgatc gtggccacat cctcgaaggc ctggcggtgg
      901 cgctgtccaa cgtcgacgag atcatcgcgc tgatcaaggc cgcgcccacg ccggccgatg
      961 ccaagcgcga gctgatggcg cgcgagtggc gctcggcgct ggtcgaggag atgctgtcgc
     1021 gcgccctggc cgacagctac cggccggaag ggctggatcc gcagtacggg ctgcaggcgc
     1081 agggttaccg cctgtccgaa gcccaggccc aggccatcct cgaattgcgc ctgcagcgtc
     1141 tgaccgggct ggagcaggac aagatcgtcg gcgaataccg cgaggtcatg gacgtcatca
     1201 ccgacctgct cgacatcctc gcccgccccg agcgcatcac cgcgatcatc gtcgaggagc
     1261 tcggcgcgat ccgcaaccag ttcggcgatc cgcgccgctc cgagctggtg atgaacaccg
     1321 ccgagatcaa catcgaggac ctgatcacgc cggaagacat ggtcgtgacc ctgtcgcaca
     1381 ccggctactt caagcgccag ccgctcgccg actaccgcgc ccagcgccgc ggtggccgcg
     1441 gcaagcaggc gacctcgatg aaggacgagg acttcatcga ccacctcttc gtcgccaaca
     1501 ctcacgacac ggtgctgtgc ttctccaacc gcgggcgctg ctactggctc aaggtgtacg
     1561 aggtccccga gggcacgcgc aactcgcgcg gcaagccgat cgtgaacctc ttcccgctgg
     1621 tggaaggcga gaagatcacc gcggtgctgc cgatcaagga gttcgacgag gagcacttcg
     1681 tgttcatggc gacctcggaa ggcacggtca agaagaccgc gctcaccgcg ttctccaatc
     1741 cgcgcaaggc cggcatcatc gcggtcaacc tggacgacgg cgaccacctg atcggggtgg
     1801 cgatcaccga cggccagtgc gacgtgatgc tgttctccga cgccggcaag gcggtgcgct
     1861 tcgccgagac cgacgtgcgc ccgatggggc gcgacgcgcg cggcgtgcgc ggcatgacgc
     1921 tggaggacgg tcaggcggtg atcgcgatgc tggtggccaa ggacgagtcg cagtcggtgc
     1981 tgaccgcgac cgagaacggc tacggcaagc gcaccccggt ggcggaatac acccgccacg
     2041 gccgcggcac caagggcatg atcgcgatcc agaccagcga ccgcaacggc aggctggtgg
     2101 gcgcggtgct ggtcgagccg agcgacgagg tgatgctgat ctccaccggc ggcgtgctga
     2161 tccgcaccag cgtcgagagc atccgcgaga tgggccgctc gacccagggc gtgaccctga
     2221 tcaacctgga cgagggcacc ttcctcgcca gcatcgagaa ggtggccgag tccgagtcgg
     2281 acgagagcat gcgtgccagc ggcgagggcg ccgagggcgg cgaggccgaa ggcggcgcac
     2341 aagccgcgcc gggcgagggc gaggcccccg ctgcggacga ggaaggagag gcctgatgag
     2401 tcgcgtgtgg aacttcagcg ccggtcccgc ggtgctgccc gaagaggtgc tgcgccaggc
     2461 cgccgacgag atgctcgact ggcacggcag cggcatgggc gtcatggaga tgagccatcg
     2521 cggcaaggaa ttcaccgcga tcgccgccca ggccgaggcc gacctgcgcg agctgctcgc
     2581 ggtgccggcc ggctaccgcg tcctcttcat gcagggcggg gccatcgccg agaacgcgat
     2641 catcccgatg aacctgatgg gcgacaggcg cgtggccgac tacgtggtca ccggttcgtg
     2701 gtcgcagaag tcgcagaagg aagcgcgcaa gtacgccgag gtgaacatcg ccgcctcgtc
     2761 cgaggcgggc ggcttcacca ccgtgccggc gatggcggac tggaggctgt cggccgaccc
     2821 ggcctacgtg ttcacctgca ccaacgagac catcggcggc ctcgagtacc ccttcgagcc
     2881 cgacctcgcg cagatcggcc gcggcgaggt gccggtggtg gccgacatgt cctcgcacat
     2941 cctgtcgcgt gtgatcgacg tctcgaagta cggcctcatc ttcggcggcg cgcagaagaa
     3001 catcggcccg gcgggtctca ccatcgtgat cgtgcgcgag gacctgctcg ggcgcgcgat
     3061 gccgcactgc cccaccgcct tcgacttcaa gaccgtggcc gacaacggct cgatgtacaa
     3121 cacgccgccc acctacggca tctacatcgc cgggctggtc ttccagtggc tcaagcgcca
     3181 gggcggggtg gcggcgatcg aggcgcagaa cgtcgccaag gcgaagctgc tgtacgactt
     3241 cctcgacgcc agcgatttct acgagaatcg catcgacccg gcctgccgtt cgcgcatgaa
     3301 cgtgcccttc ttcctcaagg acgagacctt gaacgacgcc ttcctcgccg aggccaaggc
     3361 cgccgggctg gtgcagctga agggtcacaa gtcggtcggc ggcatgcgcg cgtcgatcta
     3421 caacgcgatg ccgctcgcgg gcgtgcaggc gctggtcgac ttcatgcgcg acttcgccgc
     3481 ccgccgcggc tgaggccgcg cggggcaggg caaggcggga cggaaacacg ggacaacatc
     3541 aggacgcaag ccggaggggc cacgggccga gcatgagcga cgaactgctg aaactgcgca
     3601 acgagatcga ccgcatcgac gaggaaatcc tcgcccgcct cgccgagcgc gcgcgctgcg
     3661 cgcagcgcgt gggcgagatc aagcgcgggg tgatgtatta ccgccccgag cgcgaggcgc
     3721 aggtgctgcg ccggctggcc gagctcaacc ccggcccgct gtcttccgat gcggtgaaga
     3781 ccatcttccg cgaggtgatg tcggcctgcc tcgggctgga gcagccgctg cgcgtggcct
     3841 acctcggccc cgccggcacc ttctccgaga gcgccagccg caagcatttc ggctccgcgc
     3901 ccaacttcct gccaatggcg gcgatcgacg acgtcttccg cgcggtggaa gccggcaatg
     3961 ccgactacgg cgtggtgccg gtggagaact ccaccgaggg cgcggtcggc ggcacgctcg
     4021 atctgctgct ggccaacccg ctcaaggtct gcggcgaggt gcgcctgcgc atccaccagc
     4081 agctgatgtc gcgcgccgag ggcatcggcg ccgcccgccg catctactcg cacgcgcagt
     4141 cgctggcgca gtgccacgag tggctcaacc gcaacctccc gcacctgccg cgcatcccgg
     4201 tggcgagcaa cgccgaggcc gcgcgcatgg cctccgagga tccagagtcc tgcgccatcg
     4261 ccggcgacgc cgcggcgcag ctctacgggc tcaacatcct cgcgcccaac atcgaggacg
     4321 atcccaacaa cacgacgcgc ttcctggtca tcgccgacca cgacgccggg ccctcgggca
     4381 aggaccgcac ctcgctggtg ttctccgcgc ccaaccggcc gggggcgatc cacagcctgc
     4441 tcgagccgat ggcccgccac ggcgtggaca tgaccaagct gcagtcgcgc ccggcgcgct
     4501 ccgggctgtg ggagtacgtg ttctacgccg acatcaacgg ccaccgcgaa gaccccgagg
     4561 tggcggccgc gctgcgcgag ctcgacgagc gcgccgcctt cgtgaagatc atcggttcct
     4621 atccggtcgc ggcgatctga gcgcggcccc gattgcacac caggcccgcg cggggagtgc
     4681 gggccgcagc aggagacgac ccatgagcat tgccagtctt gcccccgact acatccgcgc
     4741 gatcatggcc taccagccgg gcaagccgat ctcggagctc gcccgcgaga tgggaatccc
     4801 cgaggagagc atcgtcaagc tcgcctccaa cgagaacccg ctcggcatga gcgcgcgcgc
     4861 gcgcgatgcc gcgatcgccg cgatcggcga ggtctcgcgc tatccggacg gcggcgcgtt
     4921 cgcgctcaag aaggccttgt gcgaacgctt cggcgtcaag cccgagcagc tcgtgatcgg
     4981 caacggctcg aacgacatcc tcgagctggc ctcgcaggcc ttcctcgcgc cggggctgtc
     5041 ggcggtgtat tcgcgtcacg ccttcgcggt ctatccgctc gcgaccaacg cgcgcggcgc
     5101 gcgcggcatc gaggtggcgg cgaagaactt cggccacgac ctcgacgcca tggcggcggc
     5161 gatcgagccg cagacccgtg tcgtcttcat cgccaacccg aacaacccca ccggcacctt
     5221 cgtcccgggc gccgagctcg aggccttcct cgccaaggtc ccgcgccacg tgctggtggt
     5281 gctcgacgag gcctacaccg aatacctcgc ccccgagcag cgctacgact cgatcgcctg
     5341 gctggcgcgc ttccccaacc tgctggtgtc gcgcaccttc tccaaggcct acggcctggc
     5401 cggcctgcgc gtgggctacg gcatcgccca ccccgaggtg gccgacctga tgaaccgggt
     5461 gcgccagccc ttcaacgtgt cctcggtggc gctcgccgcg gccgaggcgg cgctcggcga
     5521 cgacgaattc ctcgcccgca gcgccgagct caaccgccgc ggcatgacgc agctcgtcgc
     5581 cgccttccgc gaactcggcc tcgaatggat cccctcggcc ggcaacttcg tcaccttcaa
     5641 ggtgggcgac gcgatcgggg tgaaccaggc gctgctgcgc cagggcgtga tcgtgcgtcc
     5701 gatcgccgcc tacggcatgc cgcactggct gcgcgtgtcg atcggtctgc ccgaggaaaa
     5761 cgcgcgcttc atcgaggcgc tgcgccaggc gctcgcctga gcagggggcg gacgatggcg
     5821 cacatcggca agctggtcgt ctgcggggtc gggctgatcg gcggctcctt cgcgctcgcg
     5881 ctgcggcgcg cgggcgcggt cgagcgcatc gtcggcatcg gccgccggcg cgaagtgctc
     5941 gagcgcgcgt gcgcgctcgg cgtgatcgac gagatcgccg agggctgggc ggatgcgctc
     6001 gacggcgccg acctggtgct gctcgccgcg ccggtggggc agaccgacgc gatcctcgcc
     6061 gcgatggcgc cgcacctgcg cccgggcacc atcgtcaccg acgccggcag caccaaacgc
     6121 gacgtggtcg ccgcgttgcg cacccatctg ggtggcgcgc tcgccgacgt ggtgccggcg
     6181 cacccgatcg ccggcgccga gaagagcggg gtcgaggccg ccttcgccga gctctacatg
     6241 ggccgcaagg tggtgctgac gccgctgccc gagagtcgcc ccgaggccgt ccagaaggtg
     6301 cgcgacgcgt gggaggcctg cggtgccaac gtggtcggca tgacaccgca ggagcacgac
     6361 cgcgtcttcg ccgcggtgag ccacctgccg cacctgctcg ccttcggcct ggtcgacgac
     6421 ctcgccgggc gcagcaacgc gccgctgctg ttctcgcatg cggcgagcgg tttccgcgac
     6481 ttcacccgca tcgccggcag tcatcccgag atgtggcgcg acatctgcgt cgccaaccgg
     6541 gtcgcgctgc tcgaggaact cgatgcctac ctcggcgaac tcgcgcgcct gcgcatgatg
     6601 ctggtcgagg gcgatggcga cggcctggag gcggtgttcg agcgcgcccg gcgcgcgcgc
     6661 aacgcctggg ccgaggggct gccgatccgt accgccgagt gagcctgcgc tttttcctgt
     6721 ttcgcgggga cggccccgtt ttctgaggtc atgatggaat ttctcgatct gcccccgatg
     6781 ctgggcgccg ccggcagcgt ccgcctgccc ggctcgaaga gcatctccaa ccgcgtgctg
     6841 ctgctggccg cgctcgccga gggcgagacc gacatccgcg acctgctgct gtcggacgac
     6901 gtcgaacgca tgctcgaggc cctgcgcgcg ctcggcgtcg attggcggcg cgagggcgac
     6961 agcctggatt accgtgtatg cggcgtcggc ggccccttcc cggtcaagac cggcgatctc
     7021 ttcctcggca acgccggcac cgccttccgg ccgctcaccg cggcgctcgc gctgtcgggc
     7081 ggcgagtacc gcctgtcggg cgtaccgcgc atgcacgagc gcccgatcgg cgacctcgtc
     7141 gacgcgctgc gccagctcgg cgccgacatc acctgcaccg ccaacgaggg ctaccctccg
     7201 ctgcatctca agcccgccac gatccgtccc ggtggcgtgg tgcgcgtgcg tggcgatgtc
     7261 tccagccagt tcctcaccgc gctgctgatg gcgctgccgc tgaccggagt ggagaccacg
     7321 atcgaggtgg tgggcgagct catctccaag ccctacatcc gcatcaccct cgagctgatg
     7381 gcgcgcttcg gcgtgcaggt cgggcagcag ggctgggagc gcttcgtggt gccgggcggc
     7441 gcgcgctacc gcagcccggg cacggtgttc gtcgagggcg acgcctcgtc ggcatcctat
     7501 ttcctcgccg ccggggcgat cggtggtggg ccggtgcggg tggaaggcgt ggggcggacc
     7561 agcatccagg gcgacgtgcg cttcgccgag gcgctggagc aacttggcgc gcgcatcacc
     7621 ctgggcgaca actggatcga ggccgcggcg cccgccggcg gcgtgctgaa ggccttcgac
     7681 ctcgacctca accacatccc cgacgcggcg atgacgctgg cggtggcggc gctgttcgcc
     7741 gacgggccgt gccggctgcg caacatcgcg agctggcggg tcaaggagac cgaccgcatc
     7801 gccgcgatgg cgaccgagct gcgcaagctc ggcgccgagg tggaggaggg cgccgactac
     7861 ctggtggtgc agcgcccgcc gcgcctgcag ccggcggcga tcgacaccta cgacgaccac
     7921 cgcatggcga tgtgcttctc gctggcgagc ctggggggct gccgcgtgcg catcaacgac
     7981 ccgaagtgcg tgaacaagac cttccccggc tatttcgagg cctttgcgca ggtggcgcgg
     8041 ccggtgccgg tgctggccat cgatggtccc tcggcctcgg gcaagggcac ggtggcggcg
     8101 cgcgtcgccg aggcgctggg ctggcactac ctcgacagcg gctcgctcta ccgcctggtg
     8161 gcgctggccg cgatgcgcgc gggagtggcc ttcgacgacg aggccggtgt ggccgcgctc
     8221 gccgcgggtc tgccggcgcg cttcgagggc gggcgggtgc tgttgggaga gggcgcggac
     8281 cgcgacgtca ccgacgagat ccgctccgag gcctgctcgg tcggcgcctc gaaggtcgcc
     8341 gtgctgccgg cggtgcgtgc ggccctgctc gaccgccagc gcgactaccg cgcggccccc
     8401 ggcctggtgg ccgaaggccg cgacatgggc tcggtgatct tccccgatgc agggctcaag
     8461 gtcttcctta ccgcctcggc cgaggcgcgt gccgaacgcc gccataagca gttgatcgaa
     8521 aagggtttgg ctgctaacat ggaaagtctt ctgaaagacc ttcaggaacg ggatgcgcgc
     8581 gatgccgccc ggcctgtggc gccactcgtg aagctgccgg atgcggccct gctcgacacc
     8641 actcaacggg acgtcgacga ggccgtcgcc ttcgtgctcg atctcgtcgg gcgggggggc
     8701 aagtcgtccg gttgaacgca atcgcgcggg agcgtgggct ccggcgcgtt ttttcttgaa
     8761 ccagccatgt ttgccgtccc acggcggtgc cggaatccac cttttatgtc caacgccacc
     8821 cctgccttcg aagaaagctt tgccgccctc ttcgaggaaa gcctcgccct ccaggaaatg
     8881 cgcgccggtg aagtcatcac cgccgaagtc gtgcgcatcg accagaactt cgtcgtcgtc
     8941 aacgccggcc tgaagtccga aagctacgtg cccatcgagg agttccgcaa cgaccgcggc
     9001 gaactcgaag ccaacatcgg cgacttcgtc cacgtcgcca tcgacgccct cgaagacggc
     9061 tacggcgaga cccgcctgtc gcgcgacaag gccaagcgca tcgccgcctg gaacgacctc
     9121 gagaaggcgc tcaacgaagg caccctggtc aagggcgtca tcaccggccg cgtcaagggt
     9181 ggcctgaccg tcatgaccaa cagcatccgc gccttcctgc cgggctcgct ggtcgacatg
     9241 cgtccggtca aggacaccac gccgtacgaa ggcaaggaat acgagttcaa ggtcatcaag
     9301 ctcgaccgca agcgcaacaa cgtcgtggtc tcgcgtcgcg ccgtgctgga agagtcgatg
     9361 ggcgaagagc gcgagaagct gctcgccaac ctcaaggaag gcaccgtcat caagggcgtg
     9421 gtcaagaaca tcaccgacta cggtgcgttc gtggacctcg gcggcatcga cggcctgctg
     9481 cacatcaccg acctggcctg gc
//
LOCUS       DAOQBS010000117         9485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_6614_length_9485_cov_15.334995, whole genome shotgun sequence.
ACCESSION   DAOQBS010000117 DAOQBS010000000
VERSION     DAOQBS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6614_length_9485_cov_15.334995"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..2704)
                     /gene="pepN"
                     /locus_tag="PKN25_12025"
     CDS             complement(29..2704)
                     /gene="pepN"
                     /locus_tag="PKN25_12025"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HNV91670.1"
                     /translation="MKTEHAPTIQRADYRPLAWTVETVDLHFRLDPEATLVTNRMSCV
                     RKRDAADGPIRLWSEALERVSLSVDGQAPQALREDGGVIEIDAAGDRVVVEVVTRVNP
                     RANTTLSGLYLSNGGFFTQCEAEGFRRITCFPDRPDVMARYTVTLEADREACPVLLSN
                     GNLVDAGLLPGGRHYAKWEDPFRKPSYLFALVAAKLVALERKVETMSGREVLLQVWVE
                     EGNLDRVEHAMDSLVHSMRWDEETFGLELDLDRFMIVAVADFNMGAMENKGLNIFNTK
                     FILAKPDTATDLDYENVESVVAHEYFHNWTGNRVTCRDWFQLTLKEGLTVFRDQQFSA
                     DMLARAAGPEGAASARAVKRIDDVRVLRAAQFPEDGGPMAHPIRPDSYQEINNFYTAT
                     VYEKGAEVIRMLHTLLGQEGFRNGMDLYFSYFDGQAVTCDDFVEVMSEANNGFDLDQF
                     MRWYEQAGTPRVKASGEWNAADGSYTLTLAQSTPATPGQHVKLPLVIPVALGLIGADG
                     RDLPLRLEGEAQAGATTRVLKLTEEAQVFRFVGLAAEPVPSLLRGFSAPVILELDEDD
                     ARLAFRMAHDADPCNRWDAAQRYAERVALALAADPAATVPEAFVRAFRALLNDAALDP
                     AFRAQALALPGEAYLLERMSPADPAALRGALVRMMRALGATLAADWLEVVERLQVAGE
                     YRYHPGDAGRRALVNLALRYLAAAGVAEGLARAEARFAAATNMTERFGALAALVHSTS
                     PARTAALQAFHDRHRDDALVLDKWFALQAGAWRWGEAAEPALARVRVLLSDPAFSLSN
                     PNKVYALLGTFFRANPGEFHAADGSGHAFWAEQVIALDARNPQVAARMARALENWRRF
                     TPALQASIRPQLERVLATPGLSADVAEIVGKAVGE"
     gene            complement(2866..3762)
                     /locus_tag="PKN25_12030"
     CDS             complement(2866..3762)
                     /locus_tag="PKN25_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV91671.1"
                     /translation="MNPIRTLVAPTDLSALARHAVVRACLLAAELGARVSLQHVVNSG
                     ALDTLRQLLDTDSVGLQEKLLEEVRGEVEALAAEMHKRHGVTPELHLAVGGVLGEIVA
                     HAEAIDADLLVMGARGAGFMRELLIGSTTERVLRKSVRPMLVVKQIAHEPYRRVLVPV
                     DFSARALEALEFARRVAPQAEFVLLHAFEVPFEGKLRYAGVEESALSSLRVNARREAG
                     AQMNELVARAGVDENRVRRIVVHGEATTQILEQEQEQDCDLIVIGKRGHGLLGEMLLG
                     SVTKHVLARSTADVLVCDRKPD"
     gene            complement(3759..5546)
                     /locus_tag="PKN25_12035"
     CDS             complement(3759..5546)
                     /locus_tag="PKN25_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNV91672.1"
                     /translation="MAGMLAHGPYAEFAVLLIVSALAGAFAVRLRQPVLIAYIVVGIL
                     VGPAVFGIVTAHDEVALLAEIGVTVLLFVVGLKLDLHHIRHIGPVALATGLGQLGFTI
                     VFGFLIIVLMGKSLMDALYVAVALTFSSTIIVVKLLSDKRELDSLHGRIAVGFLIVQD
                     VAVVLAMMTMSALRGHGDDAILAVAGHLALRLVGAGMLMFVLMRYVLPWLVHAMARTQ
                     ELLLIFAIAWGTGLAALGELAGFSKEAGAFLAGFSLASTSYREAMNARLTGIRDFMLL
                     FFFVDLGAKLDFSTLGGELWPAVILSLFVLIGNPLIVMAIMGYMGYRKRTGFLAGLTV
                     AQISEFSIVFVAMGITLGHVGVEALGLTTLVGLVTIMVSTYMILYSQPLYERLAPLLG
                     VFERARPFRELAVERQGRAAGQPDIVVFGLGRYGARLMRQLRQAGIAVIGVDFDPEIV
                     RQLRRQRLPVRFGDGEDPGFLESLPLADARWVVTTFPQWDSNRAFLHALGEAGFKGRI
                     AGVVRDDIHGAALGAAGVERVLNPFNDAADYAARAFAAEIAYEDAHRTDAPAAAGTQS
                     ATPAQDPAAAQPAASPRANAPDRPQETER"
     gene            complement(5694..6248)
                     /locus_tag="PKN25_12040"
     CDS             complement(5694..6248)
                     /locus_tag="PKN25_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNV91673.1"
                     /translation="MTRSKSRAGKAVPDGARAAQPPARDASSLKDTAGAGPADRPSAE
                     LAGLRRRLASMLYESMLLLGVLALTFLVPYLMLGIAAGFAPPGWLLWLHVFGVLGGYF
                     VWYWRRGGQTLAMQTWRLKIVTGADGRIASLGRCWLRYALAWPSVLLGGIGLAWALVD
                     RDRQFLHDRLAGTCIVLLPAPAKA"
     gene            6499..8211
                     /locus_tag="PKN25_12045"
     CDS             6499..8211
                     /locus_tag="PKN25_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HNV91674.1"
                     /translation="MVLSGAEIVIRCLQEEKVEYVFGYPGGAVLFLYDALFQQETVRH
                     VLVRHEQAAVHAADGFARSTEKVGVALVTSGPGATNAVTGIATAYCDSIPMVIISGQV
                     PVAAIGQDAFQEVDTVGITRPCVKHNFLVRDVRDIAATMKKAFHLARTGRPGPVLVDI
                     PKDVSMQRCEYEYPKEISMRSYNPVTKGHSGQIKKAVQLLLEAKRPMIYTGGGVVLSN
                     AAEQLVKLTRLLGYPITNTLMGLGGYPSSDRQFLGMPGMHGTFEANMAMHYSDVLLAV
                     GARFDDRVIGNPAHFAEEPRKIIHVDIDPSSISKRVRVDTPIVGDVKEVLDEMIRQIE
                     SGEARPNPEALASWWKQIEQWRERKCMVYKNSDEIIKPQFVIQKLWEVTGGDAIVTSD
                     VGQHQMWAAQYYGFDKPRRWLNSGGLGTMGVGMPYAMGAKLANPDMQVACVTGEASIQ
                     MNIQELSTCKQFRLPIKICNLNNRYLGMVRQWQELFHGGRYSESYMDSLPDFAKLAES
                     YGHVGIRVDRPADVEGALREAFTTHKDELVFLDFQIDQTENVYPMVQGGKGLTEMILS
                     AEDL"
     gene            8243..8734
                     /gene="ilvN"
                     /locus_tag="PKN25_12050"
     CDS             8243..8734
                     /gene="ilvN"
                     /locus_tag="PKN25_12050"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNV91675.1"
                     /translation="MRHVISLLIENESGALSRVSGLFSARGYNIESLTVAPTEDFSMS
                     RMTIVTTGSDEIIEQITKQLNKLVEVVKVVDISEAAHIERELMLVKVRATGKDREEIK
                     RTADIFRARIIDVTDASYVVELTGNQGKLDAFIGAIDPALILETVRSGVCGIGRGDRV
                     LKV"
     gene            8776..>9485
                     /gene="ilvC"
                     /locus_tag="PKN25_12055"
     CDS             8776..>9485
                     /gene="ilvC"
                     /locus_tag="PKN25_12055"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNV91676.1"
                     /translation="MKVYYDKDADLSLIKGKQVTIVGYGSQGHAHAQNLRDSGVNVTV
                     GLRKDGSSWAKAEAAGLAVKEIGEAVKAADLVMILLPDETAPQVYRDDIEPNIKKGAV
                     LAFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYLRGGGVPTLIAIYQDRSGKA
                     KDIALSYSAANGGTKGGVIETNFREETETDLFGEQAVLCGGAVELVKMGFETLVEAGY
                     APEMAYFECLHELKLIVDL"
BASE COUNT         1602 a         3273 c         3289 g         1321 t
ORIGIN      
        1 ttgtcgagcc gagcggacgc ctcgcggctc actcccccac cgccttgccg acgatctcgg
       61 ccacgtccgc cgacagcccc ggcgtggcga gcacgcgttc gagctgcggg cggatgctgg
      121 cctgcagcgc aggcgtgaaa cgacgccagt tctccagcgc gcgggccatg cgggcggcga
      181 cctgcgggtt gcgggcgtcg agcgcgatca cctgctcggc ccagaaggcg tggccgctgc
      241 cgtcggcggc gtggaactcg cccgggttgg cgcggaagaa ggtgcccagc agggcgtaga
      301 ccttgttcgg gttcgacagg ctgaaggcgg ggtcgctgag gagcacgcgc acgcgcgcca
      361 gcgccggctc ggcggcctcg ccccagcgcc aggcgccggc ctgcaaggcg aaccacttgt
      421 cgagcacgag ggcgtcgtcg cgatggcggt cgtggaaggc ctgcagcgcg gcggtgcgcg
      481 ccgggctcgt gctgtgcacc agcgcggcga gcgcgccgaa gcgctcggtc atgttggtgg
      541 cagcggcgaa gcgtgcctcg gcccgcgcca ggccctcggc cacgccggcg gcggcgaggt
      601 agcgcagggc gagattcacc agcgcgcgac ggccggcgtc gcccgggtgg tagcgatatt
      661 cacccgctac ctgcaggcgc tcgaccacct ccagccagtc ggcggcgagg gtggcgccga
      721 gcgcgcgcat catgcgcacc agcgcgccgc gcagcgcggc gggatcggcg ggcgacatgc
      781 gctcgagcag ataggcctcg cccggcagcg cgagcgcctg ggcgcggaag gcggggtcga
      841 gcgcggcgtc gttcaacagg gcgcggaagg cgcggacgaa ggcctccggc accgtcgccg
      901 cggggtcggc cgcgagcgcc agtgcgacgc gctcggcgta gcgctgcgcg gcgtcccagc
      961 ggttgcaggg gtctgcgtcg tgcgccatgc ggaaggccag gcgcgcatca tcctcgtcga
     1021 gttcgaggat caccggcgcg gagaagccgc gcagcagcga gggcaccggc tcggcggcga
     1081 ggccgacgaa gcggaacacc tgcgcctcct cggtcagctt cagtacgcgc gtggtcgccc
     1141 cggcctgggc ctcgccctcg agccgcagcg gcaggtcgcg cccgtccgca ccgatgaggc
     1201 ccagcgcgac cggaattacc agcggcaact tgacgtgctg gccgggcgtg gccggggtcg
     1261 attgcgccag ggtcagcgtg tagctgccgt cggccgcgtt ccactccccg ctcgccttca
     1321 cccgcggcgt gccggcctgc tcgtaccagc gcatgaactg gtcgaggtcg aagccgttgt
     1381 tggcctcgga catgacctcg acgaagtcgt cgcaggtcac cgcctggccg tcgaagtagc
     1441 tgaagtagag gtccatgccg ttgcggaagc cctcctgccc gagcagggtg tgcagcatgc
     1501 ggatgacctc ggcgcccttc tcgtagaccg tggcggtgta gaagttgttg atctcctggt
     1561 agctgtcggg gcggatcggg tgcgccatcg gcccaccgtc ctcggggaac tgcgcggcgc
     1621 gcagcacgcg cacgtcgtcg atgcgcttga ccgcgcgcgc cgaggccgcc ccctcggggc
     1681 cggccgcgcg cgccagcatg tcggcggaga actgctggtc gcggaagacg gtgaggcctt
     1741 ccttcagggt gagctggaac cagtcgcggc aggtgacgcg gttgcccgtc cagttgtgga
     1801 agtactcgtg cgcgaccacg ctctcgacgt tctcgtagtc gaggtcggtg gcggtgtcgg
     1861 gcttggcgag gatgaacttg gtgttgaaga tgttcagccc cttgttctcc atcgcgccca
     1921 tgttgaagtc ggccacggcg acgatcatga agcggtcgag gtcgagctcc aggccgaagg
     1981 tctcctcgtc ccagcgcatc gagtgcacca gcgaatccat cgcgtgttcg acgcggtcga
     2041 ggttgccctc ctccacccac acctgcagca gcacctcgcg gcccgacatc gtctcgacct
     2101 tgcgttcgag cgcgaccagc ttcgccgcga ccagcgcgaa cagatacgag ggcttgcgga
     2161 agggatcctc ccacttggcg tagtggcgcc cacccggcag caggcccgcg tcgaccaggt
     2221 tgccgttgga gagcagcacc gggcaggcct cgcggtcggc ttcgagcgtg acggtgtagc
     2281 gcgccatcac gtcgggacgg tcggggaaac aggtgatgcg gcggaagccc tcggcctcgc
     2341 actgggtgaa gaagccgccg ttcgacaggt agaggcccga gagcgtggtg ttggcgcgcg
     2401 gattcacccg ggtgacgacc tcgaccacga cgcgatcgcc cgcagcgtcg atctcgatga
     2461 cgccgccgtc ctcgcgcagc gcttgcgggg cctggccgtc tacgctcagc gacacgcgct
     2521 cgagcgcctc gctccatagc cggatcgggc cgtcggcggc gtcgcgcttg cgcacgcagg
     2581 acattcggtt ggtgaccagg gtggcctcgg ggtcgaggcg gaagtgcagg tcgacggtct
     2641 cgaccgtcca cgccagcggc cggtagtcgg cgcgctggat cgtcggggca tgttcggttt
     2701 tcattatgtt ctccatcggc gcctcgcggg cgcggcaagg gcgcgagtgt acgccctggc
     2761 gggcgagccg cacagggcgg gcatgcctgg cgcaaagctg cggtcaggac cggtcaggac
     2821 gacgggacca cggcgcgagc ggcgccaggc tgcccgcggc gcggctcagt cgggcttgcg
     2881 gtcgcagacc agcacatcgg ccgtcgaacg cgcgagaacg tgcttggtga cgctgccgag
     2941 cagcatttcg ccgagcaggc cgtggccgcg cttgccgatc acgatcaggt cgcaatcctg
     3001 ttcctgctcc tgctcgagga tctgcgtcgt ggcctcgccg tggaccacga tgcggcgcac
     3061 gcggttctcg tccacccccg cgcgcgcgac cagctcgttc atctgcgcac ccgcctcgcg
     3121 gcgcgcgttg acgcgcagcg aggacagcgc gctctcctcc acgccggcat agcgcagctt
     3181 gccctcgaag ggcacctcga aggcgtgcag cagcacgaac tcggcctgcg gggccacgcg
     3241 ccgcgcgaac tccagcgcct caagcgcgcg tgccgagaag tccaccggca cgagcacgcg
     3301 gcgatagggc tcgtgggcga tctgcttgac caccagcatc gggcgcacgg acttgcgcag
     3361 cacgcgctcg gtggtggagc cgatcagcag ctcgcgcatg aagcctgcgc cgcgcgcccc
     3421 catcaccagc aggtcggcgt cgatcgcctc ggcgtgggcg acgatctcgc cgagcacgcc
     3481 acccaccgcc aggtgcagct cgggcgtcac cccgtggcgc ttgtgcatct cggcggcgag
     3541 cgcttcgacc tcgccgcgca cctcctcgag cagcttctcc tgcagcccga cggagtcggt
     3601 gtcgagcagc tggcgcaagg tgtcgagcgc gccggaattg accacgtgct gcagcgacac
     3661 ccgcgcaccg agctcggccg cgagcaggca ggcacggacc acggcatgac gggcgagcgc
     3721 cgagaggtcg gtgggggcga cgagggtgcg gatcgggttc atcgttcggt ctcctggggc
     3781 cggtcgggcg cgttcgcccg cgggctcgcc gcgggctgcg cggcggccgg atcctgcgcc
     3841 ggcgttgcgg actgcgtgcc ggcagctgcg ggtgcgtccg tgcggtgagc gtcctcgtag
     3901 gcgatctccg ccgcgaaagc gcgcgccgcg tagtcggccg catcgttgaa ggggttgagc
     3961 acgcgctcca ccccggcggc gccgagcgcg gcgccatgga tgtcgtcacg caccacgccg
     4021 gcgatgcggc ccttgaagcc ggcctcgccg agcgcgtgca ggaaggcgcg gttggaatcc
     4081 cactgcggga aggtggtcac cacccagcgc gcatcggcga gcggcaggga ttcgaggaag
     4141 ccggggtcct caccgtcgcc gaagcgcacc ggcaggcgct gacggcgcag ctggcggacg
     4201 atctcggggt cgaaatcgac gccgatcacg gcgatgccgg cctggcggag ctggcgcatc
     4261 aggcgcgcgc catagcggcc gaggccgaac accacgatgt cgggctgccc cgccgccctg
     4321 ccctggcgct cgaccgcgag ctcgcggaag gggcgcgcgc gctcgaacac gccgagcagc
     4381 ggcgcgaggc gctcgtagag cggctgcgag tacaggatca tgtaggtgga caccatgatc
     4441 gtgaccagcc cgaccagggt ggtgagaccg agcgcctcta caccgacgtg accaagggtg
     4501 atgcccatcg ccacgaacac gatggagaac tcgctgatct gcgccacggt gaggccggca
     4561 aggaagccgg tgcgcttgcg gtagcccatg tagcccatga tcgccatgac gatcaacggg
     4621 tttccgatca acacgaagag cgacaggatc accgccggcc agagctcacc gccgagcgtg
     4681 gagaaatcga gtttggcacc gaggtcgacg aagaagaaca gcagcatgaa gtcgcggatg
     4741 ccggtcaggc gcgcgttcat cgcctcgcgg tagctcgtcg aggcgagcga gaagccggcc
     4801 aggaaggcgc ccgcctcctt gctgaaaccg gccagctcgc cgagcgcggc gagcccggtg
     4861 ccccaggcga tggcgaagat cagcagcagc tcctgcgtgc gcgccatcgc gtgcaccagc
     4921 cagggcagca cgtagcgcat cagcacgaac atcagcatgc cggcgcccac caggcgcagc
     4981 gccaggtgcc cggccacggc gaggatggcg tcgtcgccgt ggccgcgcag cgcgctcatg
     5041 gtcatcatcg ccagcaccac ggcgacgtcc tgcacgatga ggaaacccac cgcgatgcgc
     5101 ccgtgcaggg agtcgagctc gcgcttgtcc gagagcagct tgaccacgat gatcgtgctg
     5161 gagaaggtca gcgccaccgc gacgtagagc gcgtccatca ggcttttgcc catcagcacg
     5221 atgatgagga agccgaacac gatggtgaag ccgagctggc cgaggccggt ggcgagcgcg
     5281 accgggccga tgtggcggat gtgatgcagg tcgagcttga gcccgaccac gaaaagcagc
     5341 acggtgacac cgatctcggc gagcagggcg acctcgtcgt gcgcggtgac gatgccgaac
     5401 accgccgggc ccaccaggat gccgaccacg atgtaggcga tcagcaccgg ttggcggagg
     5461 cgcaccgcga acgctccggc cagggcggag acgatcagca gcacggcgaa ttcggcgtag
     5521 ggcccgtggg ccagcatccc ggccatcgtt cgatcctcgt gtcacccggc gagacacccg
     5581 gatacggggg cgcgcggagc ggaaccaaag ccttcgcacg aatggtaccc cgatccgcgc
     5641 cgccagggcc ggcggacgaa aaggcgcgcg cggaaactcg ggaaagcgcc ggatcaggcc
     5701 ttcgcgggtg cgggcagcag cacgatgcag gtgccggcca ggcggtcgtg gaggaactgg
     5761 cggtcgcgat cgaccaacgc ccacgccagg ccgatgccgc ccagcagcac cgagggccag
     5821 gcgagcgcat agcgcagcca gcagcgcccg agcgaggcga tgcgcccgtc cgcaccggtg
     5881 acgatcttca atcgccaggt ctgcatcgcg agggtctgcc cgccgcgccg ccagtaccac
     5941 acgaaatacc ccccgagcac gccgaacacg tgcagccaga gcaaccagcc gggcggggcg
     6001 aacccggcgg cgatgccgag catcaggtag ggaacgagga aggtgagcgc gagcacgccg
     6061 agcagcagca tcgattcgta cagcatcgaa gcgaggcgcc ggcgcagtcc ggcgagctcg
     6121 gcggaggggc ggtcggctgg gcctgcaccg gccgtgtcct tgagggagga agcatcccgt
     6181 gccgggggct gggcggcgcg cgccccgtcg gggacggcct tgccggcccg gctcttgctg
     6241 cgcgtcatga gtcgatccag gccgccgcat ggcggcggcg caaaaaggga aaacggtagg
     6301 ggaaccgggg aggagcgcgt gcgcgaggac ctgcgctgcg gccgccggcg gcgggatcgc
     6361 gatcgcggcc ccgcaggaca acgtcaatcg cgcgcgcgtc gccgcatgcg ctcccggcca
     6421 ccgcctgcag ccttgaccaa accgcaagcg gccggtaacg ttctgcgttt atccaaaccc
     6481 caatagagcg agttcgcgat ggtgctgtcc ggagccgaaa ttgtaatcag gtgcctgcag
     6541 gaagaaaagg tcgagtatgt cttcggttac ccgggcggcg cggtcctctt cctctacgac
     6601 gcgctcttcc agcaggaaac ggtccgccac gtgctggtac gccacgagca ggccgccgtg
     6661 cacgccgccg acggtttcgc gcgcagcacc gagaaggtcg gcgtcgcgct ggtgacctcc
     6721 ggcccgggtg cgaccaacgc cgtcaccggc atcgccaccg cctactgcga ctcgatcccg
     6781 atggtgatca tctccggcca ggtgccggtc gccgcgatcg gccaggacgc cttccaggaa
     6841 gtcgacacgg tcggcatcac ccgcccgtgc gtcaagcaca acttcctcgt cagggacgtg
     6901 cgcgacatcg ccgcgacgat gaagaaggcc ttccacctcg cccgcaccgg ccgccccggc
     6961 ccggtcctgg tcgacatccc gaaggacgtg tcgatgcagc gctgcgagta cgagtacccg
     7021 aaagagatct cgatgcgctc gtacaacccg gtgaccaagg gccactcggg ccagatcaag
     7081 aaggcggtgc agctgctgct cgaagccaag cgcccgatga tctacaccgg cggcggcgtg
     7141 gtgctctcca acgcggccga gcagctcgtc aagctcacgc ggctcctcgg ctacccgatc
     7201 accaacaccc tcatgggcct cggcggctac ccctcgagcg accgccagtt cctcggcatg
     7261 ccgggcatgc acggcacctt tgaagccaac atggcgatgc actactccga cgtgctgctc
     7321 gccgtcggcg cccgcttcga cgaccgcgtg atcggcaatc cggcgcactt cgccgaagag
     7381 ccgcgcaaga tcatccatgt ggacatcgac ccctcgtcga tctccaagcg cgtgcgcgtc
     7441 gacactccga tcgtcggcga cgtcaaggaa gtgctcgacg agatgatccg ccagatcgaa
     7501 tccggcgagg cgcgccccaa ccccgaggcg ctggcctcgt ggtggaagca gatcgagcag
     7561 tggcgcgagc gcaagtgcat ggtctacaag aactcggacg agatcatcaa gccgcagttc
     7621 gtgatccaga agctgtggga agtcaccggc ggcgacgcca tcgtcacctc cgacgtcggc
     7681 cagcaccaga tgtgggcggc gcagtactac ggcttcgaca agccgcggcg ctggctcaac
     7741 tcgggcggcc tgggcacgat gggcgtgggc atgccctatg ccatgggcgc caagctcgcc
     7801 aaccccgaca tgcaggtggc ctgcgtgacc ggcgaggctt cgatccagat gaacatccag
     7861 gagctctcga cctgcaagca gttccgcctg ccgatcaaga tctgcaacct caacaaccgc
     7921 tacctgggca tggtgcgcca gtggcaggag ctgttccacg gcgggcgcta ctccgagtcc
     7981 tacatggact ccctgcccga cttcgccaag ctggccgagt cctacggcca cgtcggcatc
     8041 cgtgtcgacc ggccggccga cgtcgagggt gcgctgcgcg aggccttcac cacccacaag
     8101 gacgagctcg tgttcctcga tttccagatc gaccagaccg aaaacgtcta tccgatggtg
     8161 cagggcggga agggtctcac cgagatgatc ctgtcggccg aagacctttg atcgcctcct
     8221 accgaatccg gaacacatca ccatgcgtca cgtcatttcc ctcctgatcg aaaacgaatc
     8281 cggcgcgctg tcgcgcgtgt ccggcctgtt ctcggcgcgc ggctacaaca tcgaatcgct
     8341 gaccgtggct ccgaccgagg atttctcgat gtcgcggatg accatcgtca ccaccgggtc
     8401 cgacgagatc atcgagcaga tcaccaagca gctgaacaaa ctggtcgagg tcgtcaaggt
     8461 cgtcgacatc tccgaggccg cccacatcga gcgcgaactc atgctggtca aggtgcgcgc
     8521 cacgggcaag gaccgcgagg agatcaagcg caccgccgac atcttccgcg cccgcatcat
     8581 cgacgtcacc gacgcctcct acgtcgtcga actcaccggc aaccagggca agctcgacgc
     8641 cttcatcggc gcgatcgacc cggcactgat cctcgagacc gtgcgctcgg gcgtctgcgg
     8701 catcggccgc ggcgaccgcg tgctcaaggt ctgagctccc ccctcccccg ctccacatcg
     8761 aaccaaagga aagacatgaa ggtttactac gacaaggacg ccgacctctc gctgatcaag
     8821 ggcaagcagg tcaccatcgt cggctacggc tcgcagggcc acgcccatgc ccagaacctg
     8881 cgcgactccg gcgtgaatgt caccgtcggc ctgcgcaagg acggcagctc gtgggccaag
     8941 gccgaggccg ccggcctggc cgtcaaggaa atcggcgaag ccgtcaaggc cgccgacctc
     9001 gtcatgatcc tgctgccgga cgagaccgcg ccccaggtct atcgcgacga catcgagccg
     9061 aacatcaaga agggcgccgt gctcgccttc gcccacggct tcaacgtgca ctacaaccag
     9121 atcgtgccgc gtgccgatct cgacgtcatc atggtcgccc ccaagggccc cggccacacc
     9181 gtgcgctccg agtacctgcg cgggggcggc gtgccgaccc tgatcgcgat ctaccaggac
     9241 cgctcgggca aggccaagga catcgccctg tcgtactccg ctgccaacgg cggcaccaag
     9301 ggcggcgtga tcgagaccaa cttccgcgaa gagaccgaga ccgacctctt cggcgagcag
     9361 gccgtgctgt gcggcggcgc cgtcgagctg gtcaagatgg gcttcgagac cctggtggaa
     9421 gccggctacg cccccgagat ggcctacttc gagtgcctgc acgagctcaa gctgatcgtc
     9481 gacct
//
LOCUS       DAOQBS010000118         9453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_6657_length_9453_cov_17.067780, whole genome shotgun sequence.
ACCESSION   DAOQBS010000118 DAOQBS010000000
VERSION     DAOQBS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9453
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6657_length_9453_cov_17.067780"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PKN25_12060"
     CDS             complement(<1..529)
                     /locus_tag="PKN25_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV91677.1"
                     /translation="MIETDKLKAAAPAERLISAAPADRQEDAIERALRPKRLAEYVGQ
                     VKIREQLEIFIQAARNRSEALDHVLLFGPPGLGKTTLAHIVAAEMGVNMRQTSGPVLE
                     RAGDLAALLTNLEPHDVLFIDEIHRLSPVVEEILYPALEDFQIDIMIGEGPAARSVKL
                     DLPPFTLVGATTRAGM"
     gene            complement(594..1196)
                     /gene="ruvA"
                     /locus_tag="PKN25_12065"
     CDS             complement(594..1196)
                     /gene="ruvA"
                     /locus_tag="PKN25_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNV91678.1"
                     /translation="MIGRITGTLLEKNPPQILVDAHGVGYEIDVPMSTFYNLPATGGT
                     VSLHTHFVVREDGHFLFGFATEEERATFRQLLKVSGVGARMALAVLSGLSVNDLAQAV
                     ALQEAGRLVKIPGIGKKTAERLLLELRDKLGKALPSMTGARLAAAAPGAAPDAKSDIL
                     NALLALGYNEREALSAMKGLAEDVGVSDGIRQALKALSKA"
     gene            1727..2899
                     /locus_tag="PKN25_12070"
     CDS             1727..2899
                     /locus_tag="PKN25_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV91679.1"
                     /translation="MLQMKKLSVAFALCGAAALNATAHAADIKVGIALDLSGPFSALG
                     AEARDGFELAIEKLGGKLGGQPAEFIRSDFGGNPEQANQLVARFLQRDGIHFFTGPIG
                     SNAALAVGPALFAAKVPYLSANPGPSAYAGEKCNPWFFAQYQNDTYDEAAGKVANEKG
                     YKNVLLIAPNYPAGKDHLNGFKRLYKGAVKDEIYTKLGQIDYAAEIAQIRAAKPDAVF
                     FFLPGAMGINFVKQFVGGGLEDIPLIAPGFSADEDVIQAVGEPMLGVLNTAHWTHDLQ
                     NPANAAFVAAFRQKYAGRYPSVYAAMAYDTLMAMDAAVRDVGGRIDDRDAVLKALKNP
                     SFKSLRGEFQYGANNFPVQNYYLREVGKDAEGRITNKLVGTTLEKHQDAYVGACRM"
     gene            3099..4019
                     /locus_tag="PKN25_12075"
     CDS             3099..4019
                     /locus_tag="PKN25_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV91680.1"
                     /translation="MDAIFVFEQLLNGLGYGLMLFLIAAGLTLVFGIMDTMNLAHGSL
                     YMAGAYVAARVHEASGAFALAVLVAVGVTVAIAAVAEFLVIRRLYRRDHLVQVLATFG
                     IILIADDAVKAIWGVAPLLASTPAALAGPVALLEGLPYPSYRLLLIGAGLTVALGLHL
                     LVNKTRIGMLVRAGASNRQMAEHMGVRVGGVFAFVFALGAALAALAGALMAPISAVQI
                     GMGEAILIPALVVIVIGGIGSVRGAFVAALLVGLVDTAGRAFLPPALRAVLPPAVAAD
                     LGPALAGIAMYVLMAAVLSLKPAGLFPARG"
     gene            4028..4990
                     /locus_tag="PKN25_12080"
     CDS             4028..4990
                     /locus_tag="PKN25_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV91681.1"
                     /translation="MPERFPGLARRLPWLVLALLAAFPLLAPALGLEYYVGFVRRVLI
                     VAIAAASLNFILGYGGMAALGHAGFIGVGAYTVVALVEAGVSSAWLAWGSAAVLAGLF
                     AAAIGAVSLRTRGVYFIMITLAFAQMLHYLAVSLRTYGGDDGYGLYTPLALGAWLDGA
                     HAQVFYWVVLAIAAAVFALFSRVAHSRYGHALRGVRDNETRMVALGYPVYRLKLAGFV
                     AAGAVAGLAGGLLASHNAFVSPSIMHWTQSALLLVMVMLGGAGRRWGAPLGVALWLTL
                     EEVLKLYTDYWHWPLGLLLLLVVFHAPQGVAALLERRAGKEGAR"
     gene            4987..5736
                     /locus_tag="PKN25_12085"
     CDS             4987..5736
                     /locus_tag="PKN25_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV91682.1"
                     /translation="MSLFRAEGLVKRFGGLLATDHVSLAVEAGEIHALIGPNGAGKST
                     LVNLISGLLPADAGRIVLDGVELTALTPHARTRAGLARCFQISSVFRNDSVRDNLLLA
                     VQAQAGSSFRCLSRRDAEHALQERAEALAQRTGLAEVLERNAGTLAHGLQKRLDLALA
                     LAARPRLLLLDEPMAGMGPEDSQQMVALIRGLAGETAILLIEHDMDAVFQLADRISVL
                     VYGKVLTSGDAAHIKGHAEVQVVYLGTEVAV"
     gene            5733..6446
                     /locus_tag="PKN25_12090"
     CDS             5733..6446
                     /locus_tag="PKN25_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV91683.1"
                     /translation="MNAPLLCCRGLEAGYGTSRVLFGLDFDIRAGEVVALLGRNGMGK
                     STTIRTIVGGLRPQRGEIVFDGRPIHAERADAIARLGVAIVPEGRQCFPNLTVHEHLV
                     AFAARRNGVAEPWTPARLYALFPRLAERADHLGNQLSGGEQQMLAIARALSTNPRLLI
                     LDEATEGLAPVIREEIWRCLALLKAQGQTTLVVDKYVERLLALADRNLILERGRITWQ
                     GSSAELDARRELWGRYLGV"
     gene            complement(6503..7066)
                     /locus_tag="PKN25_12095"
     CDS             complement(6503..7066)
                     /locus_tag="PKN25_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNV91684.1"
                     /translation="MSNALARPAPISVEDYLEGETRSEIKHEYLDGEVVAMGGASARH
                     GLVVTSLGLAIGPHARRKGCQLFIADMKVRVEHTGQTYFYYPDIVLACAPDDRETYYR
                     RSPCLLVEVLSPSTERIDRREKLFAYQAIPSLREYLLVDPDKRSVEIYRFGDPVRHET
                     CAEGAFRLDCLDTEITLDEVYADAEAP"
     gene            complement(7224..8678)
                     /gene="rng"
                     /locus_tag="PKN25_12100"
     CDS             complement(7224..8678)
                     /gene="rng"
                     /locus_tag="PKN25_12100"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HNV91685.1"
                     /translation="MSIEFLINFTPQETRVAIVEQGVVQELHVERTASRGIVGNLYLG
                     KVVRVLPGMQSAFIDIGLERTAFLHVADIWSDRQNGEGARPIEKILTEGQSLMVQVLK
                     DPIGTKGARLSTQVSLAGRLLVYLPQEKHIGISQRIEDEAGREALRSRLVALVPEDEP
                     GGFIVRTMAESATDAELAADIAYLRKLWREISGSTVGLFPPKLLHEDLGLGQRTLRDL
                     VTDETSRIRVDSRENFQKLAAFAAEYTPQVLPLLEHYSGDRPLFELYSVEEEIQKALA
                     RRVDLKSGGYLIIDQTEAMTTVDVNTGGFVGARNFDDTIFKTNLEASQAIARQLRLRN
                     LGGIIIIDFIDMENVEHREMVLEEFRKALSRDHTKMSINGFTALGLVEMTRKRTRESL
                     AHLLCEPCPTCSGRGEVKTARTVCYEILRELLREARQFNAREFRVLAAPNVIDLFLDE
                     ESQSLAMLSDFIGKKVSLHAEGSYTQEQYDIVLL"
     gene            complement(8692..9312)
                     /locus_tag="PKN25_12105"
     CDS             complement(8692..9312)
                     /locus_tag="PKN25_12105"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNV91686.1"
                     /translation="MTLTPRIYLASTSPRRRELLRQIGINFDVLTFRAGERGEDADVD
                     ETPLPDEAVERYVERLALAKAQAGMRRVLWRKLLPHPVLAADTTLEVDGEIVGKPREA
                     AEAHAILERLSGRRHRVLTAVAMSDGERTRSRLSVSEVAFRTLSEHDIRRYIATGEPF
                     DKAGAYGIQGHAAIFIEEIRGSYSGIMGLPLFETAALLEIFGHPVL"
BASE COUNT         1266 a         3180 c         3465 g         1542 t
ORIGIN      
        1 gcatgccggc gcgggtggtg gcgccgacca gcgtgaacgg cggcaggtcg agcttcaccg
       61 agcgtgccgc cgggccttcg ccgatcatga tgtcgatctg gaagtcctcc agcgccgggt
      121 agaggatctc ctcgaccacc ggcgacaggc ggtggatctc gtcgatgaag agcacgtcgt
      181 gcggttcgag gttggtgagc agcgcggcga ggtcgccggc gcgctcgagc accgggccgg
      241 aggtctggcg catgttcacg cccatctcgg cggcgacgat gtgggcgagc gtggtcttgc
      301 ccagcccggg cgggccgaac agcagcacgt ggtcgagcgc ctcgctgcga ttgcgcgccg
      361 cctggatgaa gatctccaac tgttcgcgga tcttcacctg gccgacgtac tcggccaggc
      421 gcttgggacg cagcgcgcgc tcgatcgcgt cttcctggcg gtcggcgggg gcggcggaga
      481 tcaggcgctc ggcgggggcg gcagccttga gcttgtcggt ttcgatcatg ggcgtgtcct
      541 ggccgagctt gcagggcggc agccgacggc gtggcgagcg gggtgcatgc gcatcatgcc
      601 ttcgacagcg ccttcagcgc ctggcggatg ccgtccgaga cgccgacgtc ctcggccagc
      661 cccttcatcg cggacagcgc ctcgcgttcg ttgtagccga gcgcgagcag ggcgttgagg
      721 atgtcgctct tcgcgtccgg ggctgcgccc ggtgcggccg cggcgagtcg cgcgcccgtc
      781 atgctgggca gcgccttgcc gagcttgtcg cgcagctcga gcagcaggcg ctcggcggtc
      841 ttcttgccga tgccggggat cttcaccagc cgcccggcct cctgcagcgc caccgcctgg
      901 gcgaggtcgt tcaccgacag cccggacagc accgcaagcg ccatgcgcgc gcccacgccc
      961 gagaccttga gcagctggcg gaaggtggcg cgttcttcct cggtggcgaa gccgaacagg
     1021 aagtggccgt cctcgcgcac gacgaagtgc gtgtgcaggc tgaccgtgcc gccggtggcg
     1081 ggcaggttgt agaaggtgct catcggcacg tcgatctcgt agccgacgcc gtgggcgtcg
     1141 acgaggatct gcggcgggtt cttttccagc agggtgccgg tgatgcgtcc gatcatgagg
     1201 ggcgggcgtg cgaaatgcgg atgtgcaagt ctagcaggcc gcaggcgtgc ggcctgcggt
     1261 ttcatacagc agcgcgggcc ggggtgcggc gtttcgtgcg gcgctaccgc tccattcatc
     1321 gccttatcgc gactcgcgtc gctcctacgg gcgcgtcgga gaacccgccg ttcttgtagg
     1381 agcgacgcga gtcgcgatgg cggcccggga gattcgtatc gatgcccaat cgaggagccg
     1441 atcgcgactt gcgtcgctcc tgcgggcgcg ttggagaacc tgccgttctt gtaggagcga
     1501 cgcgagtcgc gagtgcggcc cgggagattc gtgtcgatgc ccggtcgagg ggccgatcgc
     1561 gacctgcatc gatcctgcgg agtttgccga agccatgtcg ttcctccagg ggcggccggc
     1621 ggcgcgaggc gcggccgggc tggggtagaa tcggcggcct tcgcgcgtgg cgcggcgctt
     1681 cgacgccggc gttgcgagcg gcaaagagaa caatcgagga gaccccatgc tgcagatgaa
     1741 gaagctgtcc gtcgcgttcg ccctgtgcgg cgctgccgcc ctcaacgcca ccgcccacgc
     1801 cgccgacatc aaggtcggta tcgcgctcga cctctccggt cccttctcgg cgctcggtgc
     1861 cgaggcgcgc gacggtttcg agctggcgat cgagaagctc ggcggcaagc tcggcggcca
     1921 gccggcggag ttcatccgca gcgacttcgg cggcaacccg gagcaggcca accagctcgt
     1981 cgcgcgcttc ctgcagcgcg acggcatcca cttcttcacc ggaccgatcg gctccaacgc
     2041 cgcgctcgcc gtcggcccgg cgctgttcgc cgccaaggtg ccctacctgt cggccaaccc
     2101 cggtccgagc gcctacgccg gcgagaagtg caacccctgg ttcttcgccc agtaccagaa
     2161 cgacacctac gacgaggccg ccggcaaggt cgccaacgag aagggctaca agaacgtcct
     2221 gctgatcgcg cccaactacc cggcgggcaa ggatcatctg aatggcttca agcgcctgta
     2281 caagggcgcg gtgaaggacg agatctacac caagctcggc cagatcgact acgccgccga
     2341 gatcgcgcag atccgcgccg ccaagccgga cgcggtgttc ttcttcctgc ccggcgcgat
     2401 ggggatcaac ttcgtcaagc agttcgtcgg tggcggcctc gaggacatcc cgctgatcgc
     2461 ccccggtttc tcggccgacg aggacgtgat ccaggcggtg ggcgagccga tgctcggcgt
     2521 gctcaacacc gcgcactgga cgcacgacct gcagaacccc gccaacgccg cgttcgtcgc
     2581 cgccttccgc cagaagtacg ccgggcgcta cccgtcggtg tatgcggcga tggcctacga
     2641 caccctgatg gcgatggacg ccgcggtgcg cgacgtcggt ggcaggatcg acgaccgcga
     2701 cgcggtgctg aaggcgctca agaatcccag cttcaagtcc ctgcgcggcg aattccagta
     2761 cggcgccaac aacttcccgg tgcagaacta ctatctgcgc gaggtcggca aggacgccga
     2821 gggccgcatc accaacaagc tggtcggcac cacgctcgag aagcaccagg acgcctacgt
     2881 gggcgcgtgc aggatgtgag acccgaggtc cgcgcgcgcg gggcatgggg ccgccgtggg
     2941 agcgacgcaa gtcgcgattc accgcgatgc accatccgaa cggcagcttc atgatttgga
     3001 gctcactgct gccccgagtg cggcgggatg ctccatgggc ggatcgcgac tggcgtcgct
     3061 cctacacaga ggcttcggac gcgccgggct gaaatccgat ggatgccatc ttcgtcttcg
     3121 agcagttgct caacggcctg ggctacgggc tgatgctgtt cctgatcgcc gccgggctca
     3181 cgctcgtgtt cggcatcatg gataccatga acctcgccca cggctcgctc tacatggccg
     3241 gcgcctacgt cgccgcgcgc gtgcatgagg cgagcggcgc gttcgcgctc gccgtgctgg
     3301 tcgcggtcgg cgtgacggtg gcgatcgcgg cggtggcgga gttcctcgtc atccgccggc
     3361 tgtatcggcg cgaccacctc gtccaggtgc tcgccacctt cggcatcatc ctcatcgccg
     3421 acgacgcggt gaaggcgatc tggggcgtgg cgccgctgct ggcctcgacc ccggcggcgc
     3481 tggccgggcc ggtcgcgctc ctcgaaggcc tgccctatcc gtcctaccgc ctgctgctga
     3541 tcggcgccgg gctgacggtg gcgctcgggc tccacctgct ggtcaacaag acccgcatcg
     3601 gcatgctggt gcgcgccggc gcctcgaacc ggcagatggc ggagcacatg ggcgtgcgcg
     3661 tgggcggggt gttcgccttc gtgttcgcgc tcggcgctgc gctcgcggcg ctggcggggg
     3721 cgctgatggc gccgatcagc gcggtgcaga tcggcatggg cgaggcgatc ctgatcccgg
     3781 cgctggtggt gatcgtcatc ggcggcatcg gctcggtgcg cggcgccttc gtggccgcgc
     3841 tgctggtcgg cctggtggac accgccgggc gcgccttcct gccgccggcc ctgcgcgcgg
     3901 tgctgccgcc cgcggtggcg gccgacctcg gcccggcgct cgccggcatc gccatgtacg
     3961 tgctgatggc ggcggtgctg agcttgaaac ccgcggggct gttccccgcg cgcggctgag
     4021 ggacgcgatg cccgaacgct tccccggcct cgcgcgccgc ctgccctggc tggtgctggc
     4081 cctgctcgcg gccttcccgc tgctcgcgcc cgccctcggg ctggagtact acgtcggctt
     4141 cgtgcgccgg gtgctgatcg tggcgatcgc ggcggccagc ctcaacttca tcctcggcta
     4201 cggcggcatg gcggcgctcg gccatgcggg cttcatcggc gtgggggcct acaccgtggt
     4261 ggcgctggtc gaggccgggg tctcgtcggc ctggctggcc tggggcagcg cggcggtgct
     4321 tgccgggctg ttcgcagcgg cgatcggcgc ggtgtcgctg cgcacgcgcg gggtgtactt
     4381 catcatgatc accctcgcct tcgcgcagat gctgcactac ctcgcggtgt cgctgcgcac
     4441 gtatggcggc gacgacggct acggcctgta caccccgctg gcgctgggtg cctggctgga
     4501 cggcgcgcat gcgcaggtct tctactgggt ggtgctggcg atcgccgcgg cggtgttcgc
     4561 gctcttcagc cgggtcgcgc attcgcgcta tggccatgcg ctgcgcggcg tccgcgacaa
     4621 cgagacgcgc atggtggcgc tcggctaccc ggtctatcgg ctcaagctcg ccggcttcgt
     4681 cgccgccggc gcggtggcgg ggttggccgg tggcctgctc gccagccaca acgccttcgt
     4741 cagcccgtcg atcatgcact ggacgcagtc cgcgctgctg ctggtgatgg tgatgctcgg
     4801 cggcgccggc cggcgctggg gcgcgccgct cggggtggcg ctgtggctga cgctggagga
     4861 ggtgctgaag ctgtacacgg actactggca ctggccgctc gggctgttgc tgctgctggt
     4921 ggtgttccac gcgccgcagg gcgtggcggc gctgctcgag cgccgcgccg gcaaggaggg
     4981 cgcgcgatga gcctgttcag ggcggagggc ctggtcaagc gcttcggcgg cctgctcgcc
     5041 accgaccatg tctccctcgc cgtcgaggcg ggcgagatcc atgcgctgat cggccccaac
     5101 ggcgcgggca agtccaccct ggtgaacctg atctccggac tgctgccggc ggacgccggg
     5161 cgcatcgtgc tcgacggtgt cgagctgacc gcactgacgc cgcacgcgcg cacccgtgcc
     5221 ggcctggcgc gctgcttcca gatcagcagc gtgttccgca acgacagcgt gcgcgacaac
     5281 ctgctgctcg cggtgcaggc gcaggcgggg tcgagcttcc gctgcctgtc gcggcgcgac
     5341 gccgagcacg cgctgcagga gcgcgccgag gcgctggcgc agcgcacggg cctggccgag
     5401 gttctcgagc gcaacgccgg caccttggcc cacggcctgc agaagcggct cgacctcgcg
     5461 ctcgcgctcg ccgcgcggcc caggctgctg ctgctcgacg agcccatggc cggcatggga
     5521 ccggaggatt cgcagcagat ggtggcgctg atccgcggcc tcgccggcga gaccgcgatc
     5581 ctgctcatcg agcacgacat ggacgcggtg ttccagctcg ccgaccgcat ctcggtgctg
     5641 gtgtacggca aggtactgac ctcgggtgac gccgcccaca tcaaggggca tgccgaggtg
     5701 caggtggtgt atctggggac cgaggtggcg gtatgaacgc gccgctgttg tgctgccgcg
     5761 gcctggaggc cggctatggc accagccggg tgctgttcgg cctcgacttc gacatccgcg
     5821 ccggcgaggt ggtggcgctg ctcgggcgca acggcatggg caagagcacc acgatccgca
     5881 cgatcgtcgg cggcctgcgc ccgcagcgcg gcgagatcgt cttcgacggc cgtccgatcc
     5941 acgccgagcg cgccgacgcc atcgcccgcc tcggcgtggc gatcgtgccc gagggccggc
     6001 agtgcttccc caatctcacg gtgcacgaac acctcgtcgc cttcgccgcg cgccgcaatg
     6061 gcgtcgccga gccatggacg ccggcgcggc tctacgcgct cttcccgcgc ctggccgagc
     6121 gcgccgacca cctcggcaac cagctctccg gcggcgagca gcagatgctg gcgatcgcgc
     6181 gtgcgctgtc gaccaacccg cgcctgctga tcctcgacga agccaccgaa ggcctcgccc
     6241 cggtgatccg cgaggagatc tggcgctgcc tcgcgctcct caaggcgcag gggcagacca
     6301 cgctggtggt ggataaatac gtcgagaggc tgctggcgct cgccgaccgc aacctcatcc
     6361 tggagcgcgg gcgcattacc tggcagggca gctcggccga gctggacgcg cggcgcgagc
     6421 tgtgggggcg ctacctgggg gtgtgagcgg ggcggacgct cccgcttcgc ggcgcgaccg
     6481 gggggggccg agccggcggg cttcagggcg cttcggcgtc ggcgtacacc tcgtccagcg
     6541 tgatctccgt atccaggcag tccagccgga aggcgccctc ggcgcaggtc tcgtggcgca
     6601 ccgggtcgcc gaagcggtag atctccacgg agcgcttgtc cggatcgacg agcagatatt
     6661 cgcgcagcga ggggatggcc tggtaggcga agagcttctc gcggcggtcg atgcgctcgg
     6721 tgctcggcga cagcacctcc accagcaggc agggactgcg gcggtaatag gtctcgcggt
     6781 cgtcgggcgc gcaggccagc acgatgtcgg ggtaatagaa gtaggtctgg ccggtgtgct
     6841 cgacgcggac cttcatgtcg gcgatgaaga gctggcagcc cttgcggcgg gcgtgcggtc
     6901 cgatcgcgag gccgaggctg gtgacgacca ggccatgcct cgcgctggcg ccgcccatcg
     6961 ccaccacctc gccgtcgagg tattcatgct tgatttcgct ccgggtctcg ccttcgaggt
     7021 agtcctcgac actgatcggg gcggggcgtg ccagggcgtt gctcatgagg gcctccgttg
     7081 cggcaggtgg gcggcagatc gacgacgaat gatacggtgg cagtcctcgt gccggccagc
     7141 acgcccgcgg accgcgcggc agcgcgcggt gtcggccggg tgttcgcgcc aaggggcgct
     7201 gcgggcgggg agccccgcca cgctcacagc agcacgatgt cgtactgctc ctgggtgtag
     7261 ctgccctcgg cgtgcagcga gaccttcttg ccgatgaagt ccgacagcat cgccagcgac
     7321 tggctctcct cgtcgaggaa gaggtcgatc acgttgggcg cggcgagcac gcggaactcc
     7381 cgggcgttga actggcgtgc ctcgcgcagc agctcgcgca ggatctcgta gcacaccgtg
     7441 cgcgcggtct tgacctcgcc gcggccgctg caggtggggc agggctcgca cagcaggtgg
     7501 gcgagggatt cgcgcgtgcg cttcctcgtc atctccacca ggccgagcgc ggtgaagccg
     7561 ttgatgctca tcttggtgtg gtcgcgcgac agcgccttgc ggaactcctc cagcaccatc
     7621 tcgcgatgct cgacgttctc catgtcgatg aagtcgatga tgatgatgcc gccgaggttg
     7681 cgcaggcgca gctggcgggc gatggcctgg ctggcctcga ggttggtctt gaagatggtg
     7741 tcgtcgaagt tgcgcgcgcc gacgaagccg ccggtgttga cgtcgacggt ggtcatcgcc
     7801 tcggtctggt cgatgatcag gtagccgccg gatttgagat cgacgcggcg ggcgagcgcc
     7861 ttctggatct cctcctccac gctgtagagc tcgaagagcg ggcgatcgcc gctgtagtgc
     7921 tcgagcagcg gcagcacctg cggcgtgtac tcggcggcga aggcggcgag cttctggaag
     7981 ttctcccgcg agtcgacgcg gatgcgtgag gtctcgtcgg tgacgaggtc gcgcagcgtg
     8041 cgctggccca gcccgaggtc ctcgtgcagc agcttcggcg ggaacaggcc cacggtcgag
     8101 ccggagatct cgcgccacag cttgcgcagg taggcgatgt cggcggcgag ctcggcgtcg
     8161 gtggcggatt cggccatggt gcgcacgatg aagccgcccg gctcgtcttc gggcaccagc
     8221 gcgaccagcc ggctgcgcag cgcctcgcgc ccggcctcgt cctcgatgcg ctgggagatg
     8281 ccgatgtgct tttcctgcgg caggtagacg agcaggcggc cggcgaggct gacctgggtg
     8341 gacaggcgtg cgcccttggt gccgatcggg tccttgagca cctgcaccat caggctctgg
     8401 ccttcggtga ggatcttctc gatcggacgc gcaccctcgc cgttctggcg gtcgctccag
     8461 atgtcggcca cgtgcaggaa ggcggtgcgc tcgaggccga tgtcgatgaa ggccgactgc
     8521 atgccgggca gcacccggac gaccttgccg aggtagaggt tgccgacgat gccgcggctg
     8581 gcggtgcgct cgacgtgcag ctcctgcacc acgccctgct cgacgatcgc gacgcgggtt
     8641 tcctgcgggg tgaagttgat gaggaattcg atgctcatgg acggggtgtg ctcacaggac
     8701 ggggtggccg aagatctcga gcagggcggc ggtctcgaac agcggcaggc ccatgatccc
     8761 cgagtagctg ccgcggatct cctcgatgaa gatcgcggcg tgaccctgga tgccgtaggc
     8821 gccggccttg tcgaagggtt cgccggtggc gatgtagcgg cggatgtcgt gctcgctcag
     8881 cgtgcggaag gcgacctcgc tgaccgacag ccggctgcgc gtgcgctcgc cgtcgctcat
     8941 cgcgaccgcg gtcagcacgc ggtggcggcg gccggacagg cgctccagga tggcgtgcgc
     9001 ctcggcggcc tcgcgcggct tgccgacgat ctcgccgtcg acctccagcg tggtgtcggc
     9061 ggcgagcacc ggatgcggca gcagcttgcg ccacagcacc cgccgcatcc cggcctgggc
     9121 cttggcgagc gcgaggcgct cgacgtagcg ttcgaccgct tcgtcgggca gcggggtctc
     9181 gtcgacgtcg gcgtcctcgc cgcgctcgcc ggcgcgaaag gtcagcacgt cgaaattgat
     9241 cccgatctgg cgcagcagtt cgcggcggcg ggggctcgtg gaggcaaggt agatgcgcgg
     9301 ggtgagggtc atggcatggc gttccgcagg atccgcgacg ccggtcggcg ccggtcgggc
     9361 tcgggtggcg cgattctaac gggtttcacc gcccgcccgc gccgccgtgc cggcatcacc
     9421 gccgcaatcg cgactcgcgt cgctcccacg ggc
//
LOCUS       DAOQBS010000119         9434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_6681_length_9434_cov_16.985180, whole genome shotgun sequence.
ACCESSION   DAOQBS010000119 DAOQBS010000000
VERSION     DAOQBS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6681_length_9434_cov_16.985180"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..749
                     /locus_tag="PKN25_12110"
     CDS             123..749
                     /locus_tag="PKN25_12110"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HNV91687.1"
                     /translation="MTPPRTITVAFTGASGLPYGVRLVECLLQAGCRVWLLYSQVAQI
                     VARQEMDWNLPARQAEVEAELSARFAAAPGQLRVFGREEWFAPPASGSNPPDAMVVCP
                     CTVATLASIAGGLSQNLIERAADVVIKEGRKLVLVPRETPYSAIHLENMLKLARLGVC
                     ILPPNPGFYHHPQTVQDLVDFVVARILDQLGVPHSLMARWGEDREQAG"
     gene            791..1393
                     /locus_tag="PKN25_12115"
     CDS             791..1393
                     /locus_tag="PKN25_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNV91688.1"
                     /translation="MTPPPDTLPLFPLKTVLFPGGVLPLRVFEPRYMDMVTRCMREGG
                     SFGVCLIAAGDEVGEAAVPHPVGTEALIERWDMEQPGVLELLVRGGRRFRIVDHELER
                     DGLLVGSVRWLEEPPAEPVPAAQAELLPLLRSIVEELGDRLPPPHDFDDAAWVGARYI
                     ELLPIPLLAKQKLLELDDIVSRLEILQQFLREHGLMPKKA"
     gene            complement(1438..2517)
                     /gene="mutY"
                     /locus_tag="PKN25_12120"
     CDS             complement(1438..2517)
                     /gene="mutY"
                     /locus_tag="PKN25_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNV91689.1"
                     /translation="MSDFSIRLIEWQRKHGRHDLPWQGGHDPYRIWLSEIMLQQTRVE
                     TVIPYYERFLARFPDVAALAAAPVEDVMALWSGLGYYARARNLHRAARVVMDAHGGAF
                     PRSAAAIAGLPGIGRSTAAAIAAFAWGERAAILDGNVKRVLCRVFGIEGFPGDKAVET
                     RLWALAESLLPEHGIGRYIQAQMDLGATLCTRARPACARCPFHDDCVARRDGRVAALP
                     TARPKKVVPRRGARCAVILHQGAVLLERRPPAGIWGGLLALPELPAEVDDAQAWSAQR
                     FGLATAAPRPLAPLTHAFTHFVLELQPLLLHASAIQGLADDGALCWLPLGAHAEAALP
                     APVRRILDGLAAPGLFDEGAPARGA"
     gene            2629..3129
                     /locus_tag="PKN25_12125"
     CDS             2629..3129
                     /locus_tag="PKN25_12125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNV91690.1"
                     /translation="MTQQQASGEDFQRHFRNALGMFATGITVVTTRTPAGEPIGLTVN
                     SFNSVSLDPPLIVWSLANELPSRTLFEGCEYYAINVLAEDQADLSQRFASRLGEKFAG
                     LEFDSGVGGVPLLAGCCARFQCRNTVRHAGGDHVVFISEVVDFDRAEERAPLLYFGGA
                     YRQLRA"
     gene            complement(3163..4338)
                     /locus_tag="PKN25_12130"
     CDS             complement(3163..4338)
                     /locus_tag="PKN25_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91691.1"
                     /translation="MSTQDSTGEDPIRVLATHAAGQVLAVSLHIPDRAWMGGAVIGMA
                     PAEGVEHLRRHASHSPQAHAHALAHACAAAAGEPPPERAEQLRTERRLAAEMIDMHLR
                     RLLLDWPPSLGFEPRINRYAEFHRRLLHAGEHDNDFVLGGEVLDLVARELLAGFFNQI
                     RMPHGIGEFLDRLAGGGSLSSILGELIAHGPSTVLPEKRTALLGTLSADAWAAEVGTW
                     PSAQFIRHPRYAGEPAETGPLARHAASPLVRILLERGNRISARLFAKAIDVADCASTM
                     RHPGSAEVAPLIDACRVAPDVGLARVISARGVLLCWVRLAEGRIADCAIVPAGAWNLE
                     PDGVYCREAMCLQVTDGDPEALKKRLEWLMLAIDPSLPFVVELVAAGEGAGVAPASA"
     gene            4556..4834
                     /locus_tag="PKN25_12135"
                     /pseudo
     CDS             4556..4834
                     /locus_tag="PKN25_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            5365..6690
                     /locus_tag="PKN25_12140"
     CDS             5365..6690
                     /locus_tag="PKN25_12140"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV91692.1"
                     /translation="MNFENLGLAEELLKAVASTGYTTPTPVQQRAIPAAIAGRDLLVS
                     SHTGSGKTAAFTLPALNRIIDRRPAPGSGPRVLVLTPTRELAQQVEKAVQTYGKALRW
                     LNTACLVGGAPMFAQIKQLQRQCDVVVATPGRLLDHLTRRKIKLSDVEVLILDEADRM
                     LDMGFAEDIDAIVAATPAKRQTLLFSATLDGVVGSMATRMTRDAERIEIEVAQEDRGQ
                     IEQRLMFADDLGHKSRLLHALLGEDGMNQAVVFTATKRSADELSLALQEKGISAAALH
                     GDMHQTQRNRTLDRLRQGRIGVLVATDVAARGIDVAGISHVINFDPPRQAEDYVHRIG
                     RTGRAGRDGIAITLSGPRETGLIRAIERFTGDRLAVHTIPGMEPSPRKPAGPRPGGNS
                     ARRFGSGAKPSGSSYGRPGGDARRSGGGFSRDGHPSRSERSHDDRRSRG"
     gene            complement(6743..7939)
                     /locus_tag="PKN25_12145"
     CDS             complement(6743..7939)
                     /locus_tag="PKN25_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNV91693.1"
                     /translation="MNPTRFAAGHAAHADWRVALARALDALLLDLRAAGGHDHAGRDG
                     LPEYTLGFCYLSDRFAADADEIVAELQRRLPGVHWVGTVGIGVAATGAEHFDEPALAL
                     MLAPLPRRAFRVFSGVQPLRADAEDFHPHTALVHADGRTPDLQELLPELAERTASGYL
                     FGGLTASRGRSVQIADGVFDGGGLSGVAFSAEVGVVSRVTQGCQPIGPRRLVSRARDN
                     FVITLDGRGALDCVMEDLGLPPDMALEPVAEALSNTLVGLHPGADAELVAPAAFGADM
                     LVRNIIGLDPRAGVVAIGDEVEQGAWLIFCRRDPAAALADLRRVAREIRDELVDSDRV
                     ALGAVYVSCSGRGGPHFGRPAAELELIRDVLGDVALAGFFAGGEIAHSRLYGYTGVLT
                     VFAAPR"
     gene            complement(7936..8838)
                     /locus_tag="PKN25_12150"
     CDS             complement(7936..8838)
                     /locus_tag="PKN25_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV91694.1"
                     /translation="MPARMHYTVLEPSSTERVAIRGLEYNVRRWGPADAPKVFLLHGW
                     MDTSATFQFVVDALRHDWQLIAPDWRGYGETTWLHRPYWFPDYYADLEALLAHYAPAG
                     PVRLVGHSMGANIAGIYAGLRPQRVARLAMLDFIGLKAPADVDAPQQLGRWLDAQLAP
                     PPARIYADRAALAERLRAVNPRLTPARAEFLCRHLGRVRADGQVEIAGDPWHKIPSPA
                     VYRIEDAMACWRRVRAPVLMLVSAEGYVHDRFGDEPEELQRRIDCFADARVVTVADCG
                     HNLQHDQPEVVAAELEAFLDREIP"
     gene            8933..>9434
                     /locus_tag="PKN25_12155"
     CDS             8933..>9434
                     /locus_tag="PKN25_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNV91695.1"
                     /translation="MTDPATLRRRFLAEPLTLRNEHRDFHEWHRGRPHYLLWAIDMDL
                     PAVRARVAAAQHALDGLLLDGYRRQPHITLALCGFPARGAAPAADEFDDAWLQARFAA
                     LEAMRIAPFTLAIGGLESFSSAPFLHVDGAHAALGALRACLHTDTPHPQGEYVPHVTV
                     GLYADAL"
BASE COUNT         1368 a         3502 c         3304 g         1260 t
ORIGIN      
        1 gcagcgaagg gccgcgggtg caggcggccc ctcgcccgct tcccgcggcc gtatcgaagc
       61 cgcgcccctc gtccgccgga tggggtacgc ttcacgccat ccgccgacct tcccgcacct
      121 cgatgacgcc tccccgcacg atcaccgtcg ccttcaccgg cgcctccggc ctgccctacg
      181 gcgtgcgcct ggtcgaatgc ctgctgcagg cgggctgccg ggtgtggttg ctgtactcgc
      241 aggtcgcgca gatcgtcgcc cgccaggaga tggactggaa cctgcccgcg cgccaggccg
      301 aagtcgaggc cgagctttcc gcgcgcttcg ccgccgcgcc cgggcagttg cgcgtgttcg
      361 gccgcgagga gtggttcgcc ccgccggcct ccggctccaa cccgcccgac gccatggtcg
      421 tgtgcccatg caccgtcgcc accctcgcct cgatcgccgg cgggctcagc cagaacctca
      481 tcgagcgcgc cgccgacgtc gtcatcaagg aaggccgcaa gctcgtgctc gtgccgcgcg
      541 agaccccgta ctcggcgatc catctcgaga acatgctcaa gctcgcccgc ctcggcgtgt
      601 gcatcctgcc gcccaacccc ggcttctatc accatccgca gaccgtgcag gacctggtcg
      661 acttcgtcgt cgcccgcatc ctcgaccagc tcggcgtgcc gcacagcctg atggcgcgct
      721 ggggcgagga tcgcgagcag gccggctgaa gcctgccggc accgctgccc cctgcgcacc
      781 ggagcgtccc atgaccccgc cgcccgacac cctcccgctg tttccgctca agaccgtgct
      841 ctttcccggc ggcgtactgc cgctgcgcgt gttcgagccg cgctacatgg acatggtcac
      901 gcgctgcatg cgcgagggcg gctccttcgg cgtctgcctg atcgcggccg gcgacgaggt
      961 cggcgaagcc gccgtgccgc acccggtggg caccgaggcg ctgatcgagc gctgggacat
     1021 ggagcagccc ggcgtgctcg agttgctggt gcgcggcgga cggcgcttcc gcatcgtcga
     1081 ccacgagctc gagcgcgacg gcctcctggt gggcagcgtg cgctggctgg aggagccgcc
     1141 cgcggagccg gtgcccgccg cgcaggccga gcttctgccg ctgctgcgct cgatcgtcga
     1201 ggagctcggc gaccgcctgc cgccgccgca cgacttcgac gacgctgcct gggtgggcgc
     1261 gcgctacatc gagctgctgc ccattccgct gctcgccaag cagaaactgc tcgagctcga
     1321 cgatatcgtc agccggctgg agatcctcca gcagttcctg cgcgagcacg gcctgatgcc
     1381 gaagaaggcc tgagcgcgca cgactcgccg cggcgcgccg gtgcgttccc cgcaggctca
     1441 ggcgccgcgc gcgggcgcgc cctcgtcgaa gaggcccggt gccgcgaggc cgtcgaggat
     1501 gcgccgcacc ggcgcgggca gggcggcctc ggcgtgggcg cccagcggca gccagcacag
     1561 cgcgccgtcg tcggccaggc cttggatggc actggcgtgc agcagcagcg gctgcagctc
     1621 gagcacgaag tgggtgaagg cgtgggtgag tggtgcgagc ggccggggcg cggcggtggc
     1681 caggccgaaa cgctgggcgc tccaggcctg ggcgtcgtcc acctcggcgg gcaattccgg
     1741 cagcgccagc aggccgcccc agatccccgc cggcgggcga cgctccagca gcaccgcgcc
     1801 ctggtgcagg atcaccgcac agcgcgcacc acgccgcggc accaccttct tcgggcgcgc
     1861 ggtcggcaac gcggccacgc gcccgtcgcg acgggcgacg cagtcgtcgt ggaaggggca
     1921 gcgcgcgcag gcggggcggg cgcgggtgca cagcgtggcg ccgagatcca tctgcgcctg
     1981 gatgtagcgg ccgatcccgt gctccggcag cagcgactcg gcgagcgccc acagccgcgt
     2041 ctccaccgcc ttgtcgccgg gaaagccctc gatgccgaag acgcggcaca gcacgcgctt
     2101 gacgttgccg tcgaggatcg ccgcacgctc gccccaggcg aaggcggcga tcgccgccgc
     2161 ggtggagcga ccgatgccgg gcagcccggc gatcgccgcg gcgctgcgcg gaaaggcgcc
     2221 gccgtgcgcg tccatgacca cccgcgccgc gcggtgcagg ttgcgcgcgc gggcgtagta
     2281 gcccaggccg ctccacaacg ccatgacgtc ctcgaccggc gccgcggcga gcgcggcgac
     2341 gtcggggaag cgcgcgagga agcgctcgta gtaggggatc acggtctcga cccgggtctg
     2401 ctgcagcatg atctccgaca gccagatgcg gtaggggtcg tggccgccct gccagggcag
     2461 gtcgtggcgg ccgtgcttgc gctgccattc gatcaggcgg atggaaaaat cgctcatgtc
     2521 cccttttgtt ccttgcgttg cctgcgcgct cgacgctgcc cgcgcgcgcg gcgataatcc
     2581 cggctttact cccacccgag cagcgcggcc caccgcgccc gtccgtccat gacccaacag
     2641 caagcttccg gcgaggactt ccagcgccac ttccgcaacg cgctcggcat gttcgccacc
     2701 ggcatcaccg tggtgaccac gcgcacgccc gccggcgagc cgatcggcct tacggtcaac
     2761 tccttcaact cggtgtcgct cgacccgccg ctgatcgtgt ggagcctggc caacgaactg
     2821 cccagccgca cgctcttcga gggctgcgaa tactacgcga tcaatgtgct cgccgaggac
     2881 caggccgatc tgtcgcagcg cttcgcgagc cggctcggcg aaaagttcgc cgggctggag
     2941 ttcgactcgg gggtcggcgg cgtgccgctg ctcgcgggct gctgcgcgcg cttccagtgc
     3001 cgcaacacgg tgcgccacgc gggcggcgac cacgtggtct tcatcagcga ggtggtcgat
     3061 ttcgaccgcg ccgaagagcg cgcgccgctg ctgtacttcg gcggcgccta ccgccagctg
     3121 cgtgcctgaa ggccgcgccg cgggcgcggc ggcggtccgg gctcaggcgg atgccggcgc
     3181 cacgcccgcg ccttcccctg cggcgaccag ctcgaccacg aaaggcagcg aggggtcgat
     3241 cgcgagcatc agccactcca gccgtttctt caacgcctcg gggtcgccgt ccgtgacctg
     3301 caggcacatc gcctcgcggc aatagacgcc gtcgggctcg aggttccacg cacccgccgg
     3361 cacgatcgcg cagtcggcga tgcgaccctc ggcgaggcgg acccagcata acagcacgcc
     3421 gcgcgcgctg atcacgcgcg ccagaccgac atccggggcg acgcggcagg cgtcgatcag
     3481 cggcgccacc tctgcgctgc ccggatggcg catcgtgctc gcgcagtcgg ccacgtcgat
     3541 cgccttggcg aacaggcgcg ccgagatgcg gttgccgcgc tcgagcagga tgcgcaccag
     3601 cggcgaggcg gcgtggcgcg cgagcggtcc agtctcggcg ggctcgccgg cgtagcgcgg
     3661 gtgacggatg aattgcgcgc tcggccaggt gcccacctcg gcggcccagg cgtcggcgct
     3721 gagcgtgccg agcagcgcgg tgcgtttttc cggcagcacg gtggacgggc cgtgggcgat
     3781 cagctcgccc aggatcgagc tcaggctgcc gccgcccgcg aggcggtcga ggaactcgcc
     3841 gattccgtgc ggcatgcgga tctggttgaa gaagccggcg agcagctcgc gcgcgaccag
     3901 gtcgagcacc tcgccgccga gcacgaagtc gttgtcgtgc tccccggcat gcagcaggcg
     3961 gcggtggaat tcggcatagc ggttgatgcg tggctcgaag ccgagcgagg gcggccagtc
     4021 gaggaggagg cggcgcagat gcatgtcgat catttcggcg gccaggcggc gctcggtacg
     4081 cagctgctct gcgcgctccg gcggcggctc gcccgcggcc gccgcgcagg cgtgcgcgag
     4141 ggcgtgagcg tgggcctgcg ggctgtggct ggcgtggcgg cgcagatgct ccacgccctc
     4201 cgcgggcgcc atgccgatca ccgcgccgcc catccaggcg cggtcgggga tgtgcagcga
     4261 caccgcaagg acctgacccg cggcatgggt ggcgagaacc cggatcggat cctcgccggt
     4321 gctgtcctgt gtgctcatgt cgtcgctcgt atgcattctt gaatcaagct ggagaatacc
     4381 cgcatcgggg aagtttgttg ttcgcgtgga cggatgtgca tcgaggccgc ccgccacggg
     4441 gaggaagaag ggcgagcgtg caatgctgcg cacgccccag gatccgcgac agtgctagaa
     4501 tccgccgcgt agaccggact ttccggaact cccactcaca caccaatcga ggaaaagcat
     4561 gaataaaggt gaattcgtcg aagccctggc cgaccgtctg gatgtgcacc gcgcccaggc
     4621 cgaccgcgcg ctgtccgccg tgctcgacat catcgccgag cagctcgcca agggcgaaaa
     4681 ggtggccttc accggtttcg gctccttcga ggtttcgcag cgcgccgccc gcaccggccg
     4741 caacccgcag accggcgcca ccatcgagat cgccgcgtcg agcgtgccca agttcaccgc
     4801 cggcgccacg ctgaaggcct cggtcaacaa gtaagcgcac accgacggcg ccctcggcgc
     4861 cgtggcctgc gggcccgttc cggctgctgc cggagcgggc ccgcttgcat tccggcgccg
     4921 tgtccaaggc gggccgtgca aaataaaccg cgacagcctg ccgtccaacc ggtatactgc
     4981 ctgtcgttgt gcaatgcagc aacagggccg agagcaatac cgacgcgtcg agggacgcga
     5041 gccggagcgg cccgagggac tgtaaccaca gccccatcgc ctccgacctc ccgtcgccga
     5101 cactccgtcg ggttttccat tcgttcttcc gatcgtctgg catcgtgaca accggttggt
     5161 gccgatgcgc ggtctttcca ggcgtccatg cgccaccgga tcgaccggcc acgcccatcg
     5221 cgcgtcccgt cgcccttgcg acgtctcgtg ccgttcctgt ccgcaggcac ggcacggcgt
     5281 gatgtcattg cggcccgcct gcggcgggtc cggatcgctc cgcccgccca ttcccgcgcg
     5341 gcgggcagca agacaggaag aagcatgaac ttcgaaaacc tcggcctcgc cgaagaactc
     5401 ctcaaggccg tcgccagcac cggctacacc accccgaccc cggtgcagca gcgcgccatc
     5461 ccggcggcga tcgccggccg cgacctgctc gtctccagcc acaccggcag cggcaagacc
     5521 gccgccttca ccctgcccgc cctgaacagg atcatcgatc gccgcccggc gcccggaagc
     5581 ggtccgcgcg tgctggtgct gacccccacc cgcgagctcg cccagcaggt cgagaaggcg
     5641 gtgcagacct acggcaaggc cctgcgctgg ctcaacaccg cctgcctggt cggtggcgcg
     5701 ccgatgttcg cgcagatcaa gcagctgcag cgccagtgcg acgtcgtcgt cgccaccccg
     5761 ggccgcctgc tcgaccacct gacccgccgc aagatcaagc tgtcggacgt cgaggtgctg
     5821 atcctcgacg aggccgaccg catgctcgac atgggcttcg ccgaggacat cgacgccatc
     5881 gtcgccgcca ccccggccaa gcgccagacc ctgctgttct cggccacgct cgacggcgtg
     5941 gtgggcagca tggcgacgcg catgacgcgc gacgccgagc gcatcgagat cgaggtcgcc
     6001 caggaagacc gcggccagat cgagcagcgc ctgatgttcg ccgacgacct cggccacaag
     6061 agccgcctgc tccacgccct gctcggcgag gacgggatga accaggcggt ggtgttcacc
     6121 gccaccaagc gcagcgccga cgagctctcg ctcgcgctgc aggaaaaggg catctccgcc
     6181 gccgcgctgc atggcgacat gcaccagacc cagcgcaacc gcaccctcga ccgcctgcgc
     6241 cagggccgca tcggcgtgct ggtggcgacc gacgtcgccg cgcgtggcat cgacgtcgcc
     6301 ggcatcagtc atgtgatcaa cttcgacccg ccgcgccagg ccgaggacta cgtgcaccgc
     6361 atcggccgta ccggccgcgc cggccgcgac ggcatcgcga tcacgctctc cggcccgcgc
     6421 gagaccggcc tcatccgcgc gatcgagcgc ttcaccggcg accgcctcgc ggtgcacacc
     6481 atccccggca tggagccctc gccgcgcaag ccggccggcc cgcgcccggg cggcaacagc
     6541 gctcgccgct tcggcagcgg cgccaagccc tctggcagca gctacggccg ccccggcggc
     6601 gacgcgcgcc gcagcggcgg cggcttcagc cgcgacggcc atccttcgcg cagcgagcgc
     6661 agccacgacg accgccgctc gcgcggctga cccggcgggc ccgcccaggg cccgagcctc
     6721 gtgaagatcc cgacgggcgg cgtcagcgcg gcgcggcgaa cacggtgagc accccggtgt
     6781 agccgtagag gcggctgtgg gcgatctcgc cgccggcgaa gaagcccgcc agcgccacgt
     6841 cgccgagcac gtcgcggatc agctcgagct cggccgccgg acggccgaag tgcgggccac
     6901 cgcggcccga gcagctcaca tacaccgcgc cgagcgcgac acggtcgctg tccaccagct
     6961 cgtcgcgaat ctcgcgcgcc acgcggcgca ggtcggcgag cgccgccgca gggtcgcgcc
     7021 ggcagaagat cagccaggcg ccctgctcca cctcgtcgcc gatcgccacc acgccggcac
     7081 gtggatcgag gccgatgatg ttgcgcacca gcatgtcggc accgaaggcg gcgggggcga
     7141 cgagctccgc gtccgccccc ggatgcagcc cgaccagggt gttcgacaag gcttcggcga
     7201 ccggctccag cgccatgtcg ggcggcaggc cgagatcctc catcacgcag tcgagcgcac
     7261 cgcgaccgtc cagtgtgatg acgaagttgt cccgcgcgcg gctcaccagc cgccgcggcc
     7321 cgatcggctg gcagccctgg gtgacgcgcg agacgacgcc gacctcggcg gagaaggcca
     7381 cccctgacag gccgccgccg tcgaacacac cgtcggcgat ctgcacgctg cgcccacgcg
     7441 aggcggtcaa cccgccgaac aggtaaccgc tcgcggtgcg ctcggcgagt tcgggcagca
     7501 gctcctgcag gtccggggtg cggccgtcgg cgtgcaccag cgcggtgtgc gggtggaagt
     7561 cctcggcatc cgcacgcagt ggctgcaccc ccgagaacac gcggaaggcg cgccgcggca
     7621 ggggtgcgag catcagcgcg agcgccggct cgtcgaagtg ctcggcaccg gtcgcggcca
     7681 ccccgatgcc cacggtgccg acccaatgca cgccgggcag gcggcgctgc aactcggcga
     7741 cgatctcgtc ggcgtcggcg gcgaagcggt cgctcaggta gcagaagccg agggtatatt
     7801 cgggcagccc gtcccgcccc gcatggtcat ggcctcccgc ggcgcgcagg tcgagcagca
     7861 gggcgtcgag cgcgcgtgcg agcgccaccc gccagtcggc gtgggcggca tgtccggcag
     7921 cgaagcgggt cgggttcatg ggatctcccg gtcgaggaac gcctccagct cggccgccac
     7981 cacctcgggc tggtcgtgct gcagattgtg gccgcaatcg gccacggtga cgacgcgggc
     8041 atcggcgaag cagtcgatgc ggcgctggag ttcctccggc tcgtcgccga agcggtcatg
     8101 cacatagccc tcggcggaga ccagcatcag caccggggca cgcacacgcc gccagcacgc
     8161 catcgcgtcc tcgatgcggt agaccgcggg cgaggggatt ttgtgccagg gatcgccggc
     8221 gatctcgacc tgtccgtcgg cacgcacgcg cccgaggtgg cggcagagga actccgcacg
     8281 cgccggggtg aggcgcggat tgaccgcgcg caggcgttcc gcgagcgcgg cccggtcggc
     8341 gtagatgcgg gcaggcggcg gtgcgagctg ggcgtcgagc cagcgcccga gctgctgcgg
     8401 cgcatcgacg tcggccggcg ccttgagccc gatgaagtcg agcatcgcca gccgcgccac
     8461 gcgctgcggg cgcagaccgg cgtagatgcc ggcgatgttg gcgcccatgc tgtggccgac
     8521 caggcgcacc gggccagcgg gcgcgtagtg ggcgagcagg gcctcgaggt cggcgtagta
     8581 gtcgggaaac cagtaggggc ggtgcagcca ggtggtctcg ccgtagccgc gccagtcggg
     8641 ggcgatcagc tgccagtcgt gacgcaacgc atcgacgacg aactggaagg tggcggaggt
     8701 atccatccag ccatgcagca ggaagacctt cggcgcgtcg gccgggcccc agcggcgcac
     8761 gttgtattcg aggccgcgga tggcgacgcg ttcggtggag gagggctcga ggacggtgta
     8821 gtgcatgcgg gcgggcatct tttccgggac aatcacgctt ccattctagc cgtcgattcc
     8881 atcccgccac cgtccgtcgg gcatcccccg ccccgccacc ccgcgctccc gcatgaccga
     8941 tcccgccacg ctgcgccggc gctttctcgc cgagccgctg accctgcgca acgagcaccg
     9001 cgacttccac gagtggcatc gcggccggcc gcattatctg ctgtgggcaa tcgacatgga
     9061 cctgccggcg gtgcgcgcgc gcgtggccgc cgcgcaacac gcgctcgacg ggctgctgct
     9121 cgacggctac cgccgccagc cgcacatcac gctggcgctg tgcggctttc cggcgcgcgg
     9181 cgcggcgccg gccgccgacg agttcgacga cgcctggctg caggcccgct tcgcggcgct
     9241 ggaggcgatg cggatcgcgc ccttcacact cgccatcggc ggcctggaga gcttctcgtc
     9301 ggcgcccttc ctgcatgtgg atggcgccca cgctgcgctg ggcgcgctgc gcgcctgcct
     9361 gcacacggac acgccgcacc cgcagggcga gtacgtgccg cacgtgaccg tgggcctgta
     9421 cgccgacgcg ctgc
//
LOCUS       DAOQBS010000120         9408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_6721_length_9408_cov_15.537795, whole genome shotgun sequence.
ACCESSION   DAOQBS010000120 DAOQBS010000000
VERSION     DAOQBS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6721_length_9408_cov_15.537795"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PKN25_12160"
     CDS             <1..258
                     /locus_tag="PKN25_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNV91696.1"
                     /translation="VAAKTKGIKSVVAGNADILVVPDLESGNMVAKQLEYLANALMAG
                     VVLGARVPIVLTSRADTAETRTASCAVVQLMAHKKREALKA"
     gene            255..1439
                     /locus_tag="PKN25_12165"
     CDS             255..1439
                     /locus_tag="PKN25_12165"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNV91697.1"
                     /translation="MKAVLVLNAGSSSLKFALYPVDGDVAAAPAVSGQVEGIGARPEV
                     TFKTAAGERIQEPVVTGGSQGEQHRDALEHVFALLVRHNPALDIVAAGHRIVHGGEHY
                     SAPTRLDEAVLRTLDTFVPLAPLHQPHNLRAVRAVATLMPQVPQVGCFDTAFHRTQPA
                     VAQAFALPRRLSAEGIKRYGFHGLSYDYVARQLPAVVGERARGAVVIAHLGNGASMCA
                     LRDGRSVASTMGFTAVEGLMMGTRTGSLDPGVMLYLMEQKGMDAKALTSLLYKESGLL
                     GVSGISQDMRTLLASDAPEAKEAVDLFCYRIARELGSLAAAAGGLDALVFTGGIGEHA
                     APVREKVAALAAWMGIEVDPAANADHARRIDTAASRVAVAVVPTNEERMIARYTAETL
                     GL"
     assembly_gap    1508..1607
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1864..3603
                     /gene="ggt"
                     /locus_tag="PKN25_12170"
     CDS             1864..3603
                     /gene="ggt"
                     /locus_tag="PKN25_12170"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNV91698.1"
                     /translation="MKRQTLLATALMMAAFGALAKQPVDTGHGGAVATISHEASLAAM
                     QILDAGGNAIDAAVAAAAALGVTDPFSCGIGGGGFMLIYSAKDRRVIAIDHREVAPAS
                     FHPGVFLDAEGKEMDWKATVPNGISVGVPGTVRGWHEALTRYGTMGFDKVLAPAIRVA
                     EEGFAVGPNFHRINKINEAKFARFSTASALYLQDGKALPVGTRFRNPELAQTYRDIAR
                     GGVKAFYEGPIAASMVAAVNHPPVTPGTSVIAGGLTMADLKDYEARIRPPIRSTYRGY
                     EIHGMPAPSSGGIAIAQALNLLETFDLAGKPRAEIEHLYLEASRLAFADRNAYVADEE
                     FVDVPKDGLLSKAYARERARQIGARAAPGKVAAGDPYPFEDDRSVPLRPQPKPLAQES
                     MHTTHLTVSDREGNVVAYTFTIEDWGGSGIVVPGRGFLLNNELTDFDFSGPHPNLPEA
                     FKRPRSSMSPTIVLKDGAPAFTIGSPGGSTIITTALQTIVNHVDLGMSLADALAAPRM
                     SQRNGETTLVETLLGFPGSEQAKALEAKGHKWRETDQIGAANAIRFNPDGTVTAVSEP
                     LRHGGGSAIVQKQ"
     gene            complement(3708..5459)
                     /locus_tag="PKN25_12175"
     CDS             complement(3708..5459)
                     /locus_tag="PKN25_12175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV91699.1"
                     /translation="MASESASGDAHPLRVVALIYLCGALLFGTLLVLEWRLIDRSVVA
                     IARERGAALFSLIQTTREWNARHGGVYVVVDESTQPNPWLKHPARDLQTTDGVRLTMV
                     NPAYMTRQIADLALHAEGARLHLTSLKPIRPENRPDAWEAMALARFEAGETEVLALVE
                     GGGEGGSGPAHRYMAPLLVREPCLQCHAEQGYQVGDIRGGISVTMPAEVLIARRDQLR
                     MRAVFVYLLAGLLAAGLMHGLLRANRRHVVQVRRINAEQERLIERRTVELAEANRRLA
                     GEVDLHRRSEQRLAASESRYRAIFDSAAEGIMLLDAALRIVQVNPAFCEITGYAAEEV
                     LGRKPSMLGSGRHDRAFFRELLATLAAAGRWQGEVWNRRKDGDLYVQWMSAVRIGEAD
                     APEGFVATMTDITSRKQTEEKLRFRANHDALTGLPNRRLFEDRLQSAIASALRHGERF
                     ALMLVDLDRFKGVNDRFGHLAGDALLVEVGRRLELCVRASDTVARLGGDEFAVILGEV
                     GGREDAAEVAERICAAMALPFELGEGVAHIGASVGIALGPQDDADAGELQRRADAALY
                     AVKHDGRGGFRFHGEPA"
     gene            complement(5527..7407)
                     /locus_tag="PKN25_12180"
     CDS             complement(5527..7407)
                     /locus_tag="PKN25_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV91700.1"
                     /translation="MPFSWFERRVDPYPEAAPGPAPRGFFAFLWAGTEGMRPFILGMM
                     LLTATIGAFEALLFAMLGRVVDWLAAVEPARLWADEGGTLLLLAAIILGSIVLVAVQT
                     LLKHQSLAGNFPMRLRWRFHRLMLGQSMSFYQDEFAGRVSAKVMQTALAVRDTCLILT
                     DILVFVSIYFFTMTAVVASFDAWLLAPFLGWLALYVLALRWFVPRLSKVSRAQADARA
                     LMTGRITDAYTNIATVKLFSHAGREAGYARGAMQEFLGTVHAQMRLVSGIEIVNHSLS
                     MGLILATGGTTLWLWSQGEVGVGAVAAATAMALRLNGMSHWVMWEMASLFENVGTVQD
                     GINTLSRPHAVVDRSDAKALVVSRGEVRFERLVFAYGATGPQARRVIDDFTLTIRPGE
                     KIGVVGRSGAGKSTLVNLLLRFYDLEQGRILIDGQDIAGVTQDSLRAQIGMVTQDTSL
                     LHRSVRDNILYGRPDATDADMIAAAKRAEAHEFILGLTDPKGRSGYDAHVGERGVKLS
                     GGQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQASLYRLMEGKTVVAIAHRLS
                     TIAAMDRLVVMDKGRIVEEGDHHSLLARGGLYARLWAHQSGGFLGEEAEDGTPLHPAC
                     PA"
     gene            complement(7545..9104)
                     /gene="ahpF"
                     /locus_tag="PKN25_12185"
     CDS             complement(7545..9104)
                     /gene="ahpF"
                     /locus_tag="PKN25_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HNV91701.1"
                     /translation="MLDANIKTQLKAYLERVTQPIEIVASLDDGPKSKELLELLHDVT
                     EQSKLITLVERRDDDERKPSFSVGPRGGEARVRFAGLPMGHEFTSLILALLQAGGYPP
                     KVEAEVIEQIRNLDVELNFETYISLSCHNCPDVVQALNLMAVLNPKIRHTMIDGALFQ
                     KEVEERQIMAVPTVYLNGEEFDQGRLELGQILAKVDTGAAARDARKLSEKPAFDVLVV
                     GGGPAGAAAAIYAARKGIRTGVVAERFGGQVMDTLAIENFISVKYTEGPKLVASLEEH
                     VKEYEVDVMNLQRVAGVVPGENQHEVKMENGASLKAKTVIVATGARWREMNVPGEKEF
                     RGKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGVVAHVTLLEFADDLRADAV
                     LQKKLYSLPNVSVIKSAQTTEVTGADKVNGLVYKDRKTNAEHRLELEGIFVQIGLLPN
                     TDFLRGTLELSRFGEVIVDAKGQTSVPGIFAAGDCTTVPYKQIIIAMGEGAKASLSAF
                     DHLIRSSAPTM"
     gene            complement(9236..>9408)
                     /locus_tag="PKN25_12190"
     CDS             complement(9236..>9408)
                     /locus_tag="PKN25_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNV91702.1"
                     /translation="KTMEVHDNAIARDVTETVRKLKAAQYVASHPNEVCPAKWKEGEA
                     TLAPSIDLVGKI"
BASE COUNT         1385 a         3329 c         3234 g         1360 t
ORIGIN      
        1 gtcgccgcca agaccaaggg catcaagtcg gtcgtggccg gcaacgccga catcctggtg
       61 gtgcccgacc tcgagtccgg caacatggtc gccaagcagc tcgaatatct cgccaacgcg
      121 ctgatggccg gcgtggtgct gggcgcgcgc gtgccgatcg tgctgaccag ccgtgccgac
      181 accgccgaga cgcgcaccgc gtcgtgcgcg gtcgtgcagc tgatggccca caagaagcgc
      241 gaggccctga aggcatgaag gccgtcctcg ttctcaatgc gggctcgtcg agcctgaagt
      301 tcgcgctcta cccggtcgac ggtgacgtgg ccgccgcgcc ggcggtgtcc ggccaggtcg
      361 aaggcatcgg cgccagaccc gaggtcacct tcaagaccgc cgcgggcgaa cgcatccagg
      421 agccggtggt gaccggcggc agccagggcg aacagcatcg cgacgcgctc gagcacgtct
      481 tcgcgctgct cgtccgccac aacccggcgc tcgacatcgt cgccgccggc caccgcatcg
      541 tgcacggcgg cgagcactac agcgcgccga cccggctgga cgaggcggtg ctgcgcacgc
      601 tcgacacctt cgtgccgctc gccccgctgc accagccgca caacctgcgc gcggtgcgcg
      661 cggtcgccac gctgatgccg caggtgccgc aggtgggctg cttcgacacc gccttccacc
      721 gcacccagcc ggcggtcgcg caggccttcg cgctgccgcg caggctgtcg gccgagggca
      781 tcaagcgcta cggcttccac ggcctgtcct acgactacgt cgcgcgccag ctgccggcag
      841 tggtcggcga gcgcgcccgc ggcgcggtgg tgatcgccca cctcggcaac ggcgcgtcga
      901 tgtgcgcgct gcgcgacggt cgcagcgtcg cgtcgacgat gggcttcacc gcggtcgagg
      961 gcctgatgat gggcacccgc accggcagcc tcgaccccgg cgtgatgctc tacctgatgg
     1021 agcagaaggg catggacgcc aaggcgctca ccagcctgct ctacaaggaa tcgggcctgc
     1081 tcggcgtgtc gggcatcagc caggacatgc gcaccctgct cgcctccgac gcccccgagg
     1141 ccaaggaagc ggtggacctg ttctgctacc gcatcgcgcg cgagctcggc tcgctggctg
     1201 cggcggcggg cgggctggac gcgctggtgt tcaccggcgg catcggcgag cacgccgcgc
     1261 cggtgcgcga gaaggtggcc gcgctggcgg cgtggatggg catcgaggtc gaccccgccg
     1321 ccaacgccga ccacgcccgc cgcatcgaca ccgcggccag ccgcgtcgcg gtcgccgtgg
     1381 tgccgaccaa cgaagagcgc atgatcgcgc gctacacggc ggagacgctg gggctgtagg
     1441 cggcgcgggc ggcgcgcatc ggccagcgcc ggcgcgccgc gcttcatcca tcggccggat
     1501 cgcgactnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct cgccggatcg
     1621 cgacttgcgt cgctcccaca agaccccgcc ggatcgcgac ttgcgtcgct cctacgcccc
     1681 gcatgcgggc acgggggtcg ggggcgctgt gggagcgacg caagtcgcga tcgcggcctc
     1741 tcggcccgcg cagtccgccc tccccgcgcg atcctttcgc cccaatccac ccgggtattc
     1801 ccgccttggg cataccctgc ggccggtgct accgtcatgc tccccagcac gacggagcac
     1861 accatgaaaa ggcaaaccct cctcgccacc gcgctcatga tggcggcgtt cggcgccctg
     1921 gcgaagcagc cggtcgacac cggccacggc ggcgcggtcg ccaccatctc gcacgaggcc
     1981 agcctggccg cgatgcagat cctcgacgcc ggcggcaacg ccatcgacgc cgcggtggcc
     2041 gcggcggccg cgctcggcgt caccgacccg ttcagctgcg gcatcggcgg cggcggcttc
     2101 atgctgatct actccgcgaa agaccggcgc gtgatcgcca tcgaccaccg cgaggtcgcc
     2161 ccggcgagct tccaccccgg cgtcttcctc gacgccgagg gcaaggagat ggactggaag
     2221 gccaccgtgc ccaacggcat ctcggtcggg gtgcccggca cggtgcgcgg ctggcacgag
     2281 gcgctgacgc gctacggcac gatgggcttc gacaaggtgc tcgcgcccgc catccgcgtg
     2341 gccgaagaag gcttcgcggt cggccccaac ttccaccgca tcaacaagat caacgaggcc
     2401 aagttcgcgc gcttctccac cgcgtcggcg ctctacctgc aggacggcaa ggcgctgccg
     2461 gtgggcacgc gtttccgcaa ccccgagctc gcgcagacct accgcgatat cgcgcgcggc
     2521 ggggtgaagg ccttctacga gggcccgatc gcggcgagca tggtggcggc ggtgaaccac
     2581 ccgccggtca cgcccggcac gagcgtgatc gccggcggcc tcaccatggc cgacctgaag
     2641 gactacgagg cgcgcatccg tccgccgatc cgctcgacct atcgtggcta cgagatccac
     2701 ggcatgcctg cgccctcgag cggcggcatc gcgatcgcgc aggcgctcaa cctgctcgag
     2761 accttcgacc tcgccggcaa gccgcgcgcc gagatcgagc acctctacct cgaggcctcg
     2821 cgcctggcct tcgccgaccg caacgcctac gtggccgacg aggagttcgt ggatgtgccc
     2881 aaggacggcc tgctgtcgaa ggcctacgcg cgcgagcgcg cgcggcagat cggcgcccgc
     2941 gcggcgccgg gcaaggtcgc ggcgggcgac ccctacccct tcgaggacga ccgctcggtg
     3001 ccgctgcgcc cgcagccgaa gccgctcgcg caggagtcga tgcataccac ccacctcacg
     3061 gtgtccgacc gcgagggcaa cgtggtcgcc tacaccttca ccatcgagga ctggggcggc
     3121 agcggcatcg tggtgccggg gcgcggcttc ctgctcaaca acgagctcac cgacttcgac
     3181 ttcagcggcc cccaccccaa cctgcccgag gccttcaagc gcccgcgctc gagcatgagc
     3241 ccgaccatcg tgctgaagga cggcgcgccc gcgttcacga tcggctcgcc cggcggctcg
     3301 accatcatca ccaccgcgct gcagaccatc gtcaatcacg tcgacctggg catgagcctg
     3361 gccgacgcgc tcgccgcccc gcgcatgagc cagcgcaacg gcgagacgac cctggtcgag
     3421 accctgcttg gcttcccggg cagcgagcag gccaaggcgc tggaggcgaa gggccacaag
     3481 tggcgcgaga ccgaccagat cggcgccgcc aacgccatcc gcttcaaccc cgacggcacg
     3541 gtgaccgcgg tcagcgagcc gctgcgccac ggcggcggca gcgcgatcgt gcagaagcag
     3601 tgaggcggca ccggcccggg cgcgaagacc aggccgcgcg gcgcgaatcg cggccgcggc
     3661 ctgccggggg cgcctgcagg gccggcgtcc ttgtcgaaac cgaaccttca ggccggctcg
     3721 ccgtggaagc ggaagccgcc gcggccatcg tgcttgacgg catagagcgc ggcgtccgcg
     3781 cggcgctgca gttccccggc gtcggcgtcg tcctgcggac cgagggcgat gccgaccgag
     3841 gcgccgatgt gcgcgacgcc ctcgccgagc tcgaagggca aggccatggc ggcgcagatg
     3901 cgctcggcca cctcggcggc atcctcgcgg ccgccgacct cgccgaggat caccgcgaac
     3961 tcgtcgccgc ccaggcgcgc caccgtgtcg gaggcgcgca cgcacagttc caggcggcgg
     4021 ccgacctcga ccagcagcgc gtcgccggcg aggtggccga agcggtcatt gacgcccttg
     4081 aagcggtcga ggtcgaccag catcagcgcg aaacgctcgc catggcgtag cgcgctggcg
     4141 atcgccgact gcagccgatc ctcgaacagg cggcggttgg gcaggccggt gagcgcatcg
     4201 tggttggcgc ggaagcgcag cttctcctcg gtctgcttgc gcgaggtgat gtcggtcatc
     4261 gtggcgacga agccctcggg cgcgtcggcc tcgccgatcc gcaccgccga catccactgc
     4321 acgtacaggt cgccgtcctt gcgccggttc cacacctcgc cctgccagcg tccggcggcg
     4381 gccagcgtgg cgaggagctc gcggaagaag gcccggtcgt ggcgccccga gccgagcatg
     4441 gacggcttgc ggccgaggac ttcttcggcc gcgtagccgg tgatttcgca gaaggcgggg
     4501 ttcacctgga cgatgcgcag cgcggcgtcg agcagcatga tgccctcggc ggcactgtcg
     4561 aagatcgcgc ggtagcgcga ctcgctcgcc gccaggcgct gctcgctgcg ccggtgcagg
     4621 tcgacctcgc cggcgaggcg gcggttggcc tcggcgagtt cgacggtgcg ccgctcgatc
     4681 agacgctcct gctcggcgtt gatccgccgc acctggacga catggcgccg gttggcacgc
     4741 agcaggccgt gcatcaggcc ggcggcgagc agcccggcga gcagatagac gaagaccgcg
     4801 cgcatgcgca gctgatcgcg ccgggcgatc agcacctcgg caggcatcgt caccgagatg
     4861 ccgccgcgga tgtcgcccac ctggtaaccc tgctctgcat gacactgcag gcagggctcg
     4921 cgcaccagca agggtgccat gtagcgatgc gccgggccgc ttccgccctc gccgccgccc
     4981 tccaccagcg ccagcacctc ggtctcgccg gcctcgaacc gcgcgagcgc catcgcctcc
     5041 caggcgtcgg gacggttctc gggtcggatc ggcttgaggc tggtgaggtg caggcgcgcg
     5101 ccctccgcgt gcagggcgag gtcggcgatc tgccgcgtca tgtaggccgg gttgaccatg
     5161 gtcaggcgca cgccgtcggt ggtctgcagg tcgcgcgccg ggtgcttcag ccaagggttc
     5221 ggctgggtgc tctcgtccac cacgacatac accccgccat gcctggcgtt ccactcgcgg
     5281 gtggtctgga tcagcgagaa cagcgccgca ccgcgttcgc gggcgatcgc gacgacgctg
     5341 cggtcgatca gccgccattc gagcaccagc aacgttccga acagcagcgc gccgcagagg
     5401 tagatgagcg cgacgacgcg caggggatgc gcatcgccgg atgcggactc cgacgccatg
     5461 tcatcgcctc cggtggtcat gcttcattct atggagaaag cgcgcatgga agcgtgacac
     5521 ctttcctcac gcggggcagg cgggatgaag cggcgtgccg tcctcggcct cctcgccgag
     5581 gaagccgccg ctctggtgcg cccacagccg cgcatagagg ccgccgcggg cgagcaggct
     5641 gtggtggtcg ccctcctcca cgatgcggcc cttgtccatc accaccaggc gatccatcgc
     5701 cgcgatggtg gacaagcggt gcgcgatcgc caccacggtc ttgccttcca tcaggcgata
     5761 caggctggcc tggatggcgg cctcgacctc ggagtcgagc gcgctggtgg cctcgtcgag
     5821 cagcaggatg ggcgcgtcct tcagcatcac gcgcgcgatc gcgatgcgct ggcgctggcc
     5881 gccggagagc ttcacgccgc gctcgccgac gtgggcgtcg tagccgctgc gacccttggg
     5941 gtcggtaagg ccgaggatga actcgtgcgc ctcggcccgc ttcgcggcgg cgatcatgtc
     6001 ggcgtcggtg gcgtcggggc ggccatagag gatgttgtcg cgcaccgagc ggtgcagcag
     6061 cgaggtgtcc tgggtgacca tgccgatctg cgcgcgcagg ctgtcctgcg tcacgccggc
     6121 gatgtcctgg ccgtcgatca ggatgcggcc ctgctcgaga tcgtagaagc gcagcagcag
     6181 gttcaccagg gtcgacttgc ccgcgcccga gcgcccgacc acgccgatct tctcgcccgg
     6241 acggatggtc agcgtgaagt cgtcgatcac ccgccgcgcc tgcggcccgg tggcgccgta
     6301 ggcgaacacg agccgctcga agcgcacctc gccgcggctc accaccagcg ccttggcgtc
     6361 gctacggtcg accaccgcat gcgggcgcga caaggtgttg atgccgtcct gcacggtgcc
     6421 gacgttctcg aacagcgagg ccatctccca catcacccag tgcgacatgc cgttcaggcg
     6481 cagcgccatc gcggtcgccg ccgccaccgc gccgaccccg acctcgccct ggctccacag
     6541 ccacagcgtc gtgccgccgg tggcgaggat cagccccatc gacaggctgt gattgacgat
     6601 ctcgatgccg ctcaccaggc gcatctgcgc atgcacggtg ccgaggaact cctgcatcgc
     6661 gccgcgcgcg tagccggcct cgcggccggc gtgggagaac agcttgacgg tggcgatgtt
     6721 ggtgtaggcg tcggtgatgc gcccggtcat cagcgcgcgc gcgtcggcct gggcgcgcga
     6781 caccttcgac aggcgcggca cgaaccagcg cagcgccagc acatagagcg ccagccagcc
     6841 gaggaagggt gcgagcagcc aggcgtcgaa gctcgccacc accgcggtca tggtgaagaa
     6901 gtagatgctc acgaacacca ggatgtcggt aaggatcagg caggtgtcgc gcaccgcgag
     6961 cgcggtctgc atgaccttgg ccgacacccg gccggcgaac tcgtcctggt agaagctcat
     7021 gctctggccg agcatcaggc ggtggaagcg ccagcgcagc cgcatcggga agttgcccgc
     7081 caggctctgg tgcttgagca gggtctgcac cgccaccagc acgatgctgc cgaggatgat
     7141 ggccgcgagc agcagcaggg tgccgccttc gtccgcccac aggcgcgcgg gctcgaccgc
     7201 ggccagccag tcgaccacgc ggccgagcat ggcgaacagc agggcctcga aggcgccgat
     7261 ggtcgcggtg agcagcatca tgccgaggat gaagggccgc atgccctcgg tgcccgccca
     7321 caggaaggcg aagaagccgc gcggcgcggg gccgggggcg gcttcgggat aggggtcgac
     7381 gcggcgttcg aaccagctga aaggcacggc ggctctccgt gatgggtccg gcctgtgacc
     7441 ggccggatgg gccgcgggtg cccgcggcaa agcaaaaagc cccgcgcgtg gcggggcggg
     7501 gttcgcggct gccgccaccc gcgcaggagg gcggcgggcc gggcttacat ggtcggcgcg
     7561 ctgctgcgga tcaggtggtc gaaggcggac agcgaagcct tggcaccttc gcccatggcg
     7621 atgatgatct gcttgtacgg caccgtggtg cagtcgccgg cggcgaagat gcccggcacc
     7681 gaggtctggc ccttggcgtc cacgatcacc tcgccgaagc gcgacagttc gagcgtgccc
     7741 ctgaggaagt cggtgttggg cagcaggccg atctgcacga agatgccttc gagctcgaga
     7801 cggtgctcgg cgttggtctt gcgatccttg tacaccaggc cattcacctt gtcggcaccg
     7861 gtgacctcgg tggtctgcgc gctcttgatc acgctgacgt tgggcaggct gtagagcttc
     7921 ttctgcagca cggcgtcggc gcgcaggtcg tcggcgaact cgagcagggt cacgtgggcg
     7981 accacgccgg cgaggtcgat cgcggcctcg acgccggagt tgccgccgcc gatcaccgcc
     8041 acgcgcttgc ccttgaacag cgggccgtcg cagtgcgggc agtaggccac gcccttgccg
     8101 cggaactcct tctcgccggg cacgttcatc tcgcgccagc gcgcaccggt ggcgacgatc
     8161 acggtcttgg ccttgaggct ggcgccgttc tccatcttca cctcgtgctg gttctcgccc
     8221 ggcaccacgc cggcaacgcg ctgcaggttc atgacgtcga cttcgtattc cttgacgtgc
     8281 tcctccaggc tggcgaccag cttggggcct tcggtgtact tcaccgagat gaagttctcg
     8341 atcgccagcg tgtccatgac ctggccgccg aagcgctcgg ccaccacgcc ggtgcggatg
     8401 cccttgcgcg cggcgtagat cgccgccgcc gcgccggccg gtccgccccc cacgacgagc
     8461 acgtcgaagg ccggcttctc ggagagcttc ctggcgtcgc gcgccgcggc gccggtgtcg
     8521 accttggcga ggatctggcc gagctcgagg cggccctggt cgaactcctc gccgttcagg
     8581 tacaccgtcg gcaccgccat gatctggcgc tcctcgactt ccttctggaa cagcgcgcca
     8641 tcgatcatgg tgtggcggat cttcgggttg agcaccgcca tcaggttgag cgcctgcacc
     8701 acgtccgggc agttgtggca ggacagcgag atgtaggtct cgaagttcag ctcgacgtcg
     8761 aggttcctga tctgctcgat cacctcggcc tcgaccttgg gcggatagcc gccggcctgc
     8821 agcagggcga ggatcagcga ggtgaattcg tggcccatgg gcaggcctgc gaagcgcacg
     8881 cgcgcctcgc cgccgcgcgg tccgaccgag aacgagggct tgcgttcgtc gtcatcgcga
     8941 cgctcgacca gggtgatcag cttcgactgc tcggtgacgt cgtgcagcag ctcgagcagt
     9001 tccttcgact tcggaccatc atccagcgac gccacgatct cgatcggctg cgtcacccgc
     9061 tccaggtagg ctttcagttg agtcttgatg ttggcgtcca gcatgatggt cactcccctt
     9121 ctgttttgcg accgccgctg tttacggcag tccggatacg acgcagccgg cgcgtggccg
     9181 gctgcggagc ttgcgccccg gaccacggcg gtccggggaa aggtgcagca ctgccttaga
     9241 tcttgccgac caggtcgatc gacggggcca gcgtggcttc gccttccttc cacttcgccg
     9301 ggcacacttc gttcgggtgc gaggcgacgt actgggcggc cttgagcttg cgcacggtct
     9361 cggtcacgtc acgggcgatg gcgttgtcgt gcacttccat cgtcttga
//
LOCUS       DAOQBS010000121         9162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_6991_length_9162_cov_16.378500, whole genome shotgun sequence.
ACCESSION   DAOQBS010000121 DAOQBS010000000
VERSION     DAOQBS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6991_length_9162_cov_16.378500"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="PKN25_12195"
     CDS             complement(<1..1505)
                     /locus_tag="PKN25_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNV91703.1"
                     /translation="MPSPHPHLPFLAWPAQWREHGLRGDLVAGITVALVLIPQALAYA
                     KLAGLPPHIGLYAALLPAVVAALFGSCGQLSTGPVALTSLLVGASILPLGATAGGDLV
                     GLALLLALLSGLIQLALGVLRLGWLLNLLSVPVLMGFVNAAALIICLTQIPPLLGLAM
                     PHSDHLLVDFVRALGGLDALQPAAAAFGVGSLAVLVGLKRLWPRLPGVPLVVAATIAL
                     SAWTGFAAQGGAVVGAVPAGLPGFSLPPLRPELIAALLPAAFVVAMVSFMEAASSAKL
                     ICGRTRQPWNQDQELVGQGLGKLAAAFTGGLPVSASFSRSALNYANEARTGLSSLVTA
                     AFVLAALLWLTPLLWHLPKPVLAAIILQAVAGLIDTGVMRRAWRASRDDGAAAAVTFL
                     ATLAFAPNIQNGVFTGLILSLALLVYRGMRPRVALLGLHEDGTYRDRERFGLHHPHPR
                     LVILRFDGPLSFVTAATFEDAMLGAARAQESVRVVLVSAAGINAIDATGLHT"
     gene            complement(1713..2867)
                     /locus_tag="PKN25_12200"
     CDS             complement(1713..2867)
                     /locus_tag="PKN25_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV91704.1"
                     /translation="MKARNLVVAMLAALGASSASAALVNLNGLGYVTYGDGNSYSLAV
                     ANYFACGSEYGPGCPYNVKGSPGASETDPRIYIVQGAGGHIDNENANIDDAFDAANNE
                     LFFTMTSANEPGSDGNKNATFTGDKTNTWDATLGALDQSFDFSKNLMTFFFVNNEPNS
                     NDGLTQNLAVWARVTLTNLAGTTVYKTFDVTNDPRTSEQRDGDGIGDLPAPGYGPPPV
                     GGGVPYGDVTLYTSEGNAPEITDFAMSGGQVCVDKTTSTLVDCTTASAGSFDAINHNL
                     GENQAPYAITIPELDDYIGGLMAANANLDDYVMRVDLRYGCVSPPFTIDTQGNDSCVG
                     YGTAVSQDNNFEQVFISTRLATQRVPEPGTLALLGSGLLGFAAARRSRKS"
     gene            3354..4235
                     /locus_tag="PKN25_12205"
     CDS             3354..4235
                     /locus_tag="PKN25_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNV91705.1"
                     /translation="MALRRIPPIQCLLTFEAVARLRSASRAAEELCVTVSAVSHRLRQ
                     LESHLGVRLFSRSDFTPTAEGAAYLQQVGVALAALRELPGERAPGASARLRVAVTPSF
                     CRQFVMPKLELFRRDWPDIELTLQVSIPLRDVVAEEAELEIRYGAGGYTDLEHRLLLS
                     DEAVPACSPAFLDEHGPFDGFASLAECAGTALVRSPLEPWSNWFAHCGLALPEPARGA
                     QFNDLGLAYDAAASGFGVALVRQRLGAAWFDSGRLLALSSRPLRSPYAHYLCWRPGAL
                     ERWECAAFADWLQAALP"
     gene            4549..6060
                     /locus_tag="PKN25_12210"
     CDS             4549..6060
                     /locus_tag="PKN25_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNV91706.1"
                     /translation="MMLENEALPFGETEGDFSAVDKARVVAALSKVLPSGTLMYDSED
                     LRPYECDGLAAYRALPLVVALPTTEAQVVAVLRICHEMGVPVVARGAGTGLSGGALPH
                     TRGVLLSLARFNRIVRLDPLARTAVVQPGVRNLAISEAAAPYGLYYAPDPSSQIACSI
                     GGNVAENAGGVHCLKYGLTVHNLLRVRGVTIAGEVVEIGSGALDSPGYDLLALVTGSE
                     GMLIVVTEVTVKLVPKPQLAQCVLAAFDDVVRAGEAVARIIAAGIIPAGLEMMDQPAT
                     AAVEEFVRAGYPLDAKAILLCEADGTPEEVAEEIARVRAVLEDSGATEIRVSRDEAER
                     LRFWAGRKAAFPAAGRISPDYYCMDGTIPRKRLGEMLTAIQAMEGKYGLRCMNVFHAG
                     DGNLHPLILFDANQPDELERAEEFGAEILELSVALGGTITGEHGVGLEKINQMCSQFT
                     TAEVRQFFRVKAAFDPPGLLNPGKAIPTLNRCAEYGRMKVSKGHVPYPEIPRF"
     assembly_gap    6189..6288
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6407..7477
                     /gene="glcE"
                     /locus_tag="PKN25_12215"
     CDS             6407..7477
                     /gene="glcE"
                     /locus_tag="PKN25_12215"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HNV91707.1"
                     /translation="MTMIEKEWAERVRAAVATRTPLCLRGGGSKDFFGCTLAGERFDL
                     RANRGVVAYEPTELVVTVCAGTPLAELEAVLADKGQYLAFEPPHFGPGATVGGCVTAG
                     LSGPRRASAGAVRDFMLGVKLLDGRGEVLRFGGQVMKNVAGYDVSRLVAGSLGTLGIL
                     LEVSLKVLPRPVAEATLRFELDEASALRRVNEWGGQPLPVSATLWQHGVLHLRLSGAE
                     AAVQAACARLGGEALDDATAQALWTAAREQTLPGFALEEGEVLWRLSLPSTAPALGLP
                     GRQLVEWGGAQRWLAGAIDAQAVCARAVELGGHATAFRGGDRAAGVFQPLAAPLAAVH
                     RRLKQAFDPAGIFNPGRLYPEL"
     gene            7481..8722
                     /gene="glcF"
                     /locus_tag="PKN25_12220"
     CDS             7481..8722
                     /gene="glcF"
                     /locus_tag="PKN25_12220"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HNV91708.1"
                     /translation="MQTQLADFIRDTPEGREAEEILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGAAPTEKTRLHLDRCLTCRSCESTCPSGVHYSRLLDIGRAVV
                     AERVPRHGGEALVRRTLREFVPRPKLFGLAMGAGRAVRGLLPKSLQDKVPTLRPAGTW
                     PAPRHARRVIALAGCAQPAMAPSINAAAARVLDALGISMVELPGAGCCGAVRFHLDDA
                     EGARADARRNIDAWWPDIEAGRIEAIVMTASGCGVQVRDYGHLLQHDPVYAERARRVS
                     ELTRDIAELVLAEGESLRARLAARAQPREPLAWHAPCTLQHGQRIRGTVESLLTAAGY
                     ELTPVRDAHLCCGSAGTYSILQPELSGRLRANKLDALLEGGAARIASANIGCITHLQA
                     GTDKPVLHWIELIDSRLGPAS"
     gene            8849..>9162
                     /locus_tag="PKN25_12225"
     CDS             8849..>9162
                     /locus_tag="PKN25_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HNV91709.1"
                     /translation="MPFTAETCVAMHLGDRKEQQDRVALFGHPKRPGMMMAVLADGMG
                     GHSGGAMAAEQVLMRARQNFEAYAPLHETPEHLLGSIIDEAHVVIKLTRFTSEQDPHS
                     TAV"
BASE COUNT         1316 a         3075 c         3302 g         1369 t
ORIGIN      
        1 gtgtgcaggc cggtggcgtc gatcgcgttg atgccggcgg ccgagaccag cacgacgcgc
       61 acgctctcct gcgcgcgcgc ggcgccgagc atggcgtcct cgaaggtggc ggcggtgacg
      121 aaactgagcg ggccgtcgaa acgcaggatc accagccggg gatgcgggtg gtgcaggccg
      181 aagcgctcgc ggtcgcggta ggtgccgtcc tcgtgcaggc cgagcagcgc cacgcgcgga
      241 cgcatgccgc gatacaccag cagcgcgagc gacaggatca ggccggtgaa caccccgttc
      301 tggatgttgg gcgcgaaggc gagggtggcg aggaaggtga ccgccgcggc ggcgccgtcg
      361 tcgcggctcg cacgccaggc gcgccgcatc actccggtgt cgatcagccc ggcgaccgcc
      421 tgcagtatga tcgccgccag caccggcttg ggcaggtgcc acagcagcgg ggtgagccac
      481 agcagggcgg ccagcacgaa ggcggcggtg accagcgagg acaggccggt gcgcgcctcg
      541 ttggcgtagt tgagcgccga gcgcgagaac gaggcgctca ccggcaggcc gccggtgaag
      601 gcggcggcga gcttgcccag gccctggccg acgagctcct ggtcctggtt ccaaggctgg
      661 cgggtgcggc cgcagatcag cttggcgctc gaggccgcct ccatgaagct caccatggcc
      721 accacgaagg cggccggcag cagcgcggcg atcagctccg ggcgcagcgg cggcaggctg
      781 aagccgggca ggccggccgg cactgcgccc accaccgcac cgccttgggc agcgaagccg
      841 gtccaggccg atagcgcgat cgtcgccgcc accaccagcg gcacgccggg caagcgcggc
      901 cacaggcgct tgagccccac cagcacggcc aggctgccga cgccgaaggc cgccgcggcg
      961 ggctgcagtg cgtcgagccc gccgagcgca cgcacgaaat cgaccagcag gtgatcggag
     1021 tgcggcatgg ccaagccgag tagcggaggg atctgggtca ggcagatgat cagcgcggcg
     1081 gcgttgacga agcccatcag caccggcacc gacagcagat tgagcagcca gcccaggcgc
     1141 agcacaccga gcgcgagctg gatcaaaccc gacagcagcg ccagcagcag ggcgagcccg
     1201 acgaggtcgc ccccggcggt cgcgcccagc ggcaggatgc tcgcgcccac gagcaggctg
     1261 gtgagcgcca ccggcccggt ggagagctgg ccgcaggaac cgaacagcgc agcgaccacc
     1321 gccggcagca gcgcggcgta gaggccgatg tgcggcggca gcccggcgag cttggcgtag
     1381 gcgagcgcct gcggaatcag caccagcgcg accgtgatgc cggcgacgag gtcgccgcgc
     1441 agcccatgct cgcgccattg cgcaggccag gcgaggaagg gcaggtgcgg atgtggggag
     1501 ggcatcggcc ggaggattcc cgagggcggg ggggtgtggc cccctgtcgt ccgggggatt
     1561 ccatcttagc cgtgaaacgg gggggcggcc caggcgcccg aaaagagttc gggcagcggc
     1621 gcgggacgcg cggctgcccg aagtggatcc ggcagggccg gatccggacg aggctcggtg
     1681 agggttttcg ctgccgcggt ggccgcgatg cctcaggact tgcgggagcg gcgggcggcc
     1741 gcgaaaccca gcagacccga gccgagcagc gccagggtgc cgggttccgg cacgcgttgc
     1801 gtcgccaggc gggtgctgat gaagacctgt tcgaagttgt tgtcctgcga caccgcggtg
     1861 ccgtaaccga cgcaggagtc gtttccctgc gtgtcgatgg tgaatggcgg gctgacgcag
     1921 ccgtagcgca ggtcgacccg catcacgtag tcgtcgagat tggcattcgc cgccatcagg
     1981 ccgccgatgt aatcgtcgag ttcgggaatg gtgatcgcgt agggggcctg gttctcgccg
     2041 aggttgtgat tgatcgcatc gaagctgccg gcgctggcgg tggtgcaatc gaccagcgtg
     2101 ctggtggtct tgtcgacgca cacctggccg cccgacatgg cgaagtcggt gatctcgggg
     2161 gcgttgccct cgctggtata gagcgtgacg tcaccatacg ggacaccgcc tccgaccggc
     2221 ggggggccgt aacccggggc gggcaggtcg ccgatgccgt cgccgtcgcg ctgttcgctc
     2281 gtgcgcgggt cgttggtcac gtcgaaggtc ttgtagaccg tcgtgccggc gaggttggtc
     2341 agcgtcacgc gggcccagac ggcgaggttc tgcgtcaggc cgtcgttgct gttgggctcg
     2401 ttgttcacga agaagaacgt catcaggttc ttcgagaagt cgaagctctg gtcgagtgcg
     2461 ccgagcgtcg catcccaggt gttggtcttg tcgccggtga aggtggcgtt cttgttgccg
     2521 tcgctgcccg gctcgttggc ggaggtcatc gtgaagaaca gctcgttgtt cgccgcgtcg
     2581 aaggcgtcgt cgatgttcgc gttctcgttg tcgatgtggc cgccggcccc ctggacgatg
     2641 tagatgcgcg ggtcggtctc actcgcaccc ggggagccct tgacgttgta aggacagccg
     2701 gggccgtatt cagagccgca ggcgaaataa ttggccaccg ccagcgaata gctgttgcca
     2761 tcgccgtacg tgacgtaacc gagcccgttg aggttgacca atgcggccga tgcggacgat
     2821 gcgccgagcg cggcgagcat tgcgacgacc aggtttctgg ctttcatggc actccctcct
     2881 acaggtgtat ggagatctcg tagcaaatcg tgtgccaata atgcaagtat ctgtttttta
     2941 gatattttaa tttttagatt tttcttcttc agcagcctgt gtaaaaaaat cgacacccgg
     3001 atccagccta cttgcggaga tccgaacggg ttccaagcga agggatatgc gaaaggcaat
     3061 tcctttgcgg cgccttttcc gatggccatg gaaagtaact cggagttgcc gatgtactat
     3121 gaaaaattaa ttcgataaat gcctgaagaa taattgcggc acgaatgaag tggccgggcc
     3181 gatccggcgc ttcgtcctgc gaccgggaga atgacgctct cgcgccacag caggatgcgc
     3241 gcacccgaaa ctcggcgcgg gagggaaggt gggtgggggc gtcgcagtgc aggcagcggg
     3301 gctgcggggg cataatcggg gacatccttc cgaacgggcc ccgagccgcg accatggcgc
     3361 tccgccgcat cccgcccatc cagtgcctgc tcaccttcga ggcggtcgcg cgcctgcgca
     3421 gcgcgagccg tgcggccgag gagctctgcg tgaccgtcag cgcggtcagc catcgcctgc
     3481 gccagctcga gtcccatctg ggcgtgcggc tcttctcgcg cagcgacttc accccgaccg
     3541 ccgagggggc ggcctacctg cagcaggtcg gtgtcgcgct cgcggcgctg cgcgaattgc
     3601 cgggcgagcg cgcgccgggt gcatcggcgc gcttgcgcgt ggcggtgaca ccctcgttct
     3661 gccgacagtt cgtcatgccc aagctcgagc tcttccgccg cgactggccg gacatcgagc
     3721 tcaccctgca ggtgtcgatc ccgctgcgcg acgtcgtcgc ggaagaggcg gagctcgaga
     3781 tccgctatgg cgccgggggc tacaccgacc tcgagcaccg cctgctgctg agcgacgagg
     3841 cggtccccgc ctgcagcccg gccttcctcg acgagcacgg ccccttcgac ggtttcgcga
     3901 gcctcgccga gtgcgccggc accgcgctcg tgcgcagccc gctcgagccc tggtcgaact
     3961 ggttcgcgca ctgcggtctg gctctgcccg agccggcgcg cggcgcacag ttcaacgacc
     4021 tcggcctggc ctacgatgcc gcggcgagcg gcttcggggt cgccctggtg cgccagcgcc
     4081 tgggggcggc gtggttcgac agcgggcgcc tgctggcgtt gtcctcgcgc ccgctgcgct
     4141 cgccctacgc gcactacctg tgctggcgcc ccggcgcgct cgagcgctgg gagtgcgccg
     4201 cgttcgcgga ctggctgcag gccgcgctgc cctgaagcat cgccgggccg tttcagggtt
     4261 gaattttctt caagccggcg agcaagaggc gtcaagcggg cgctgtgcgc gagcctctat
     4321 actccggata gctgcccggt cccgcgggga gtcgaggatt cggatgcgcc aaagatcgcg
     4381 acacgcccgc ggggattccg ggggcggagg atcgcgcttc gcggcggttc tgttttcgta
     4441 ggagcgacgc aagtcgcgat tgccgcggtc ccgatacgcc cgcgcggatt tcgatgtgcc
     4501 ggcgcggcgg tcgatgctcg atcggcatgg ccaggcgagg agacgaggat gatgctggag
     4561 aacgaagcgc tgccgttcgg tgagaccgag ggcgattttt ccgcggtgga caaggcgcgc
     4621 gtggtcgcag ccttgagcaa ggtgctgccg tccgggacgc tgatgtacga cagcgaggac
     4681 ctgcgcccct acgagtgcga cggcctcgcc gcctaccgcg cgctgccgct ggtcgtggcc
     4741 ctgcccacca ccgaggcgca ggtggtggcg gtgctgcgca tctgccatga gatgggcgtg
     4801 ccggtggtgg cgcgtggcgc gggcaccggg ctgtcgggcg gcgctctgcc gcacacccgc
     4861 ggcgtgctgc tgtcgctggc gcgcttcaac cgcatcgtca ggctcgatcc gctcgcacgc
     4921 accgcggtcg tgcagccggg cgtgcgcaac ctggcgatct ccgaggcggc cgcgccctac
     4981 gggctgtatt acgcgcccga tccgtcctcg cagatcgcct gctcgatcgg cggcaacgtg
     5041 gccgagaacg ccggcggcgt gcattgcctc aagtacggcc tcacggtgca caacctgctg
     5101 cgcgtgcgcg gcgtcaccat tgcgggcgag gtcgtcgaga tcggctcggg tgcgctcgat
     5161 tcgccgggct acgacctgct tgcgctggtc accggctctg agggcatgct gatcgtggtc
     5221 accgaagtca ccgtcaagct cgtgcccaag ccgcagctcg cgcagtgcgt gctcgccgcc
     5281 ttcgacgacg tggtgcgcgc cggcgaggcg gtggcgagga tcatcgccgc cggcatcatc
     5341 cccgccggcc tggagatgat ggaccagccc gcgaccgcgg cggtcgagga gttcgtgcgc
     5401 gccggctatc cgctcgacgc caaggcgatc ctgctgtgcg aggccgacgg cacgccggag
     5461 gaggtggccg aggagatcgc ccgcgtgcgc gcggtgctgg aagacagcgg cgccaccgag
     5521 atccgcgtgt cgcgcgacga ggccgagcgg ctcaggttct gggccgggcg caaggccgcc
     5581 ttcccggcgg cggggcggat ctcgcccgac tactactgca tggacggcac gattccacgc
     5641 aagcgcctcg gcgagatgct caccgccatc caggccatgg aaggcaagta cggcctgcgc
     5701 tgcatgaacg tgttccatgc cggcgacggc aacctgcatc cgctgatcct cttcgacgcc
     5761 aaccagcccg acgagctcga gcgcgccgaa gagttcggcg ccgagatcct cgagctgtcg
     5821 gtggcgctcg gcggcaccat caccggcgag catggcgtcg ggctggagaa gatcaaccag
     5881 atgtgttcgc agttcaccac ggccgaggtg cgccagttct tccgcgtcaa ggcggccttc
     5941 gatccgcccg ggctgttgaa ccccggcaag gcgatcccca cgctcaaccg ctgcgccgaa
     6001 tacggccgca tgaaggtgag caaggggcat gtgccgtatc cggagattcc gcggttctga
     6061 acgggggaat cgcgacttgc gtcgctccta cgggcggtcc cgggcggcgg tgaatcccgg
     6121 atggtcacgg ggtttccgta ggagcgacgc gagtcgcgat cgccgccgac ggactgcgcc
     6181 ccgcgcgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt ccgtaggagc
     6301 gacgcgagtc gcgatcgccg ccgacggact gcgccccgcg cgtcgggagt gcccggccgg
     6361 cagggtgtgc ggccacgcat atgaatgatg attggacgat gacacgatga cgatgatcga
     6421 gaaggaatgg gccgagcgcg tgcgcgcggc tgtggcgacg cgtacgccgc tgtgcctgcg
     6481 cggcggcggc agcaaggact tcttcggctg tacgctcgcc ggcgagcgct tcgacctgcg
     6541 cgccaaccgc ggcgtggtcg cctacgaacc caccgagctc gtcgtgaccg tgtgtgccgg
     6601 cacgccgctc gccgagctcg aagcggtgct ggcggacaag ggccagtacc tcgctttcga
     6661 accgccgcat ttcggtcccg gcgccacggt gggcggctgc gtgaccgccg gcctgtcggg
     6721 gccgcggcgg gcgagtgccg gcgcggtgcg cgacttcatg ctcggcgtca agctgctcga
     6781 cggccgtggc gaggtgctgc gcttcggcgg ccaggtcatg aagaacgtcg ccggctacga
     6841 cgtctcccgc ctggtggcgg gcagcctcgg cacgctcggc atcctgctcg aggtctcgct
     6901 caaggtgctg ccgcggccgg tggccgaggc cacgctgcgt ttcgagctcg acgaggcgag
     6961 cgcgctgcgg cgcgtcaatg aatggggcgg ccagccgctg ccggtgtcgg ccacgctgtg
     7021 gcagcacggc gtgctgcacc tgcgcctgtc gggcgccgag gcggcggtgc aggcggcctg
     7081 tgcgcggctc ggcggcgaag cgctcgatga cgcgacggcg caggcgctgt ggaccgcggc
     7141 gcgcgagcag acccttcccg gcttcgcgct ggaggagggc gaggtgctgt ggcggctgtc
     7201 gctgccctcg accgcgcccg cgctcggcct gcccgggcgc cagctcgtcg agtggggcgg
     7261 cgcgcagcgc tggctcgcgg gcgcgatcga cgcgcaggcg gtgtgtgcgc gcgccgtcga
     7321 gctcggcggc catgccacgg ccttccgcgg tggcgatcgc gccgccggcg tgttccagcc
     7381 cctggcggcg ccgctcgcgg cggtgcatcg ccggctcaag caggccttcg accccgcggg
     7441 gatcttcaac cccggccgcc tctacccgga gctctgagcc atgcagaccc agctcgccga
     7501 cttcatccgc gacacgcccg aaggccgcga ggccgaggag atcctgcgca agtgcgtgca
     7561 ttgcggcttc tgcaccgcca cctgcccgac ctaccagctg ctcggcgacg agctcgacgg
     7621 cccgcgcggg cgcatctacc tgatcaagca ggtgctcgag ggcgccgcgc ccaccgagaa
     7681 gacccgcctg cacctcgacc gctgcctcac ctgccggtcc tgcgaatcca cctgtccctc
     7741 gggtgtgcat tacagccgcc tgctcgacat cggccgcgcg gtggtggccg agcgcgtgcc
     7801 gcgccacggc ggcgaggcgc tggtgcggcg cacgctgcgc gagttcgtgc cgcggccgaa
     7861 gctgttcggc ctggcgatgg gcgccgggcg ggcggtgcgc ggcctgctgc cgaagtccct
     7921 gcaggacaag gtgcccacgc tgcggccggc cggcacctgg ccggcgccac gccatgcgcg
     7981 ccgggtgatc gcgctcgccg gttgcgcgca gcccgcgatg gcgccttcga tcaacgccgc
     8041 ggccgcgcgc gtgctcgacg cgcttggcat ctcgatggtc gagctgcctg gtgccggctg
     8101 ctgcggtgcg gtgcgcttcc acctcgacga tgccgagggc gcgcgcgccg acgcccgccg
     8161 caacatcgac gcctggtggc ccgacatcga ggccgggcgc atcgaggcga tcgtgatgac
     8221 cgcctccggc tgcggcgtgc aggtgcgcga ctacggccat ctgctgcagc acgatcccgt
     8281 ctatgcggaa cgcgcgcggc gggtgagcga gctgacgcgc gacatcgcgg agctcgtcct
     8341 cgccgagggc gagtccctgc gtgcgcggct cgccgcccgc gcgcagccgc gcgagccgct
     8401 cgcctggcac gcgccgtgca cgctgcagca cggccagcgc atccgcggca cggtcgagtc
     8461 cctgctgacg gcggcgggct acgagctcac cccggtgcgc gacgcccacc tttgctgcgg
     8521 ttcggcgggc acctactcga tcctgcagcc cgagctctcc ggccgcctgc gcgcgaacaa
     8581 gctcgacgcc ctgctcgagg gcggcgccgc acggatcgcc tccgccaaca tcggctgcat
     8641 cacccacctg caggcgggca ccgacaagcc ggtgctgcac tggatcgagc tgatcgattc
     8701 ccgtctcggc ccggcgtcgt gatgaagcgc accgggcgtg acggatttca caccgggtcg
     8761 cgccccccgg cggcaggcgc tcgcctgctg cccgctccgg gttgatatca tcctgtgatc
     8821 cgtcccgatt ccggcctgct catccctcat gcctttcacc gccgaaacct gtgtcgccat
     8881 gcacctgggc gaccgcaagg agcagcagga ccgcgtcgcg ctgttcggcc accccaagcg
     8941 gccgggcatg atgatggcgg tgctcgccga cggtatgggc ggccatagcg gcggcgccat
     9001 ggcggccgag caggtgctga tgcgcgcgcg gcagaatttc gaggcctatg cgccgctgca
     9061 cgagacgccc gagcacctgc tcggcagcat catcgacgag gcccacgtcg tcatcaagct
     9121 gacccgcttc accagcgagc aggacccgca cagcaccgcg gt
//
LOCUS       DAOQBS010000122         9050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_7109_length_9050_cov_16.682490, whole genome shotgun sequence.
ACCESSION   DAOQBS010000122 DAOQBS010000000
VERSION     DAOQBS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7109_length_9050_cov_16.682490"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..940)
                     /locus_tag="PKN25_12230"
     CDS             complement(26..940)
                     /locus_tag="PKN25_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNV91710.1"
                     /translation="MRRISLEDLHIFRCVAVEGGVLRAADRLNRVPSNVTTRIKQFES
                     RLGTQLFRRQGRNVVLTDAGHRLLGHAERLLRLADEAEEDLCSGVVAGSLRLGSLESA
                     ASVRLPPILSRYHAAHPRTHVELQTGTTAALLRRLDNHQIDAAFVSEPFDKGSLSAVA
                     VFAEELVLITARGGPRIGGAQDLADQTVVAFPHGCSYRRLLVDWFAAAGVTPRRFLDL
                     GSYHAIVACVAAGTGVAIMPASVLDNAVMGDSIQRHELPEAMRTNRTNLVWAGEPGGP
                     LSALLELLRVGAQAGACGRHGRTSKSMP"
     gene            1005..2213
                     /locus_tag="PKN25_12235"
     CDS             1005..2213
                     /locus_tag="PKN25_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HNV91711.1"
                     /translation="MEKNPPSSLSPGAIVFIGLLALAIAMGIGRFAFTPLMPLMVRDG
                     SLNTVTGTEWATANYVGYLLGAISASWFARSPRLGLQVGLLGVAATTLAMAWGNAALP
                     WLGLALRASAGVFSAWVLVCASSWCLPELARRSSTSLGGWIYTGVGVGIAATGVLTWL
                     GGAQAAAVLWVELGLLAVVGAVHVIWRLRGQSAAASPAGTPDAPKPPPPAVASGNLGI
                     VLCYSAFGFGYIIPATFLPTMARQLVSDPLVFGLTWPIFGAAAALSVAFAAHRLHGWP
                     RRKVWAIAQGVMAAGTLLPLLQPALWVLAASAVLVGGTFMVTTMAGLQLARERAPANP
                     TALLARMTTGFAAGQIAGPLLVRLVGDTRIAGLDALGLASATAGLLLAMTAIWLWRDD
                     IAAATKPRSA"
     gene            2378..2764
                     /gene="sdhC"
                     /locus_tag="PKN25_12240"
     CDS             2378..2764
                     /gene="sdhC"
                     /locus_tag="PKN25_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNV91712.1"
                     /translation="MDKPPHRLPVFLNLAQIRFPIGAIASIAHRVSGVLLFIALPVLA
                     ALLHASLRSEADFASVRGLLSSPLSLVVAGVLAWALAHHVLAGIRHLLMDVGIGSELE
                     RARASARLILVAAPALALLLLVWGLS"
     gene            2761..3102
                     /gene="sdhD"
                     /locus_tag="PKN25_12245"
     CDS             2761..3102
                     /gene="sdhD"
                     /locus_tag="PKN25_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNV91713.1"
                     /translation="MRLFTGQRAWVVQRLSALLLLVLILLAMLALPFASPLDFSAWRA
                     LAGGALGGPLIALLFGALCAHAWVGMRDIVLDYLQPRGLRLAVLGLITVVLLGVLARV
                     LLSLAMVALAA"
     gene            3120..3815
                     /locus_tag="PKN25_12250"
     CDS             3120..3815
                     /locus_tag="PKN25_12250"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNV91714.1"
                     /translation="MRLNIYRYDPERGDPPRMQAYDVQPEAGDKKLLDLLMRLRVQDD
                     SLSFRRSCREGVCGSDAMNINGRNGLACLTALDGLKEPIVLRPLPGFPVIRDLVVDMT
                     QFFAHYHSIRPWLINDEPLPERERLQTPPERERLDGLYECILCACCSAFCPSYWWNPD
                     KFIGPSGLLQAYRFIADSRDTASAERLDFLDDVYRLYRCRTIMNCTEVCPKGLSPSHA
                     IERIRLALLQRSS"
     gene            complement(3873..4448)
                     /locus_tag="PKN25_12255"
     CDS             complement(3873..4448)
                     /locus_tag="PKN25_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1282 family protein"
                     /protein_id="HNV91715.1"
                     /translation="MSSLHLPPMLWSSDAAWTELSRRPPSLATLVFGVALPAAMLPPA
                     MLSHATDVGSAHYLPALSASAWQFVEFALFAAQLVGFLMIRWLIGAVAATHGVRASAR
                     DACLLATVSLLPLWLSSFALLQDGTLAVLVLPVLAIGASVALLKRGVRCMLGVREEIV
                     ALEIGIVAIHCGVLAWAATMAVFLVPGLPAL"
     gene            complement(4631..5068)
                     /locus_tag="PKN25_12260"
     CDS             complement(4631..5068)
                     /locus_tag="PKN25_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNV91716.1"
                     /translation="MPERRITIKEVAERFDISRSHLMKIVTELVAKGFVDGARGKGGG
                     LRLARASSEIGLGNVVRQLEPSLALVECFGTQSHCLLDPACRLKGALDGALQAFLAAL
                     DRVSLADLVGSDARLHEALGLQAAARAIAPITIVRPPAGRERR"
     gene            5393..7069
                     /locus_tag="PKN25_12265"
     CDS             5393..7069
                     /locus_tag="PKN25_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91717.1"
                     /translation="MAEHAAEPTVKHFRLALLWPLRLLTATGALGPQQRPWQVLRDLG
                     EASPWREVEHDLEVEGSVFQERHYNEFVTFLPYVQQFLYGEGRAQRGDSADAGPAMRV
                     FRRRDIAKLRVAARDGDAPVTLDVVHVHLYFFFDVDVVILNVEVAGGDLPLSAVQELM
                     YRIGRAYPSGWDEHGRALHSLAVAELIGRDGSVLARTDAHEREAFLTHVSRHRAPRIA
                     AHWAWLMQPLASAYSAQPGTLRYRQIEYHRMPLTAYLALDDPQALTRRDFVRLGLVTG
                     AGPHTAQAEREGGGNALPYAEPHLDDFERRHCYDRFWSASGAAPWTRYLCSGHALVVV
                     GDAKSAYFTDRERGVLAQFRHQHFLLFLIAHFQKSALLMFSERLVEALKRLHVTDPES
                     VRRFKRAIRASFEGFLRFTHRYWFHDIAEQAQTRALFRMTSAHLGTDALYAEIKERTA
                     DMNGYLEADSLRRQANTVVRLTVVTIFGLIGTITTGFLGMNLLAEAESPLAERLAYFA
                     LVFGVSTALTLYTMVKSKRLSDFLDAVSDERLSTWNKLRALAAVWRRGAD"
     gene            complement(7079..8227)
                     /locus_tag="PKN25_12270"
     CDS             complement(7079..8227)
                     /locus_tag="PKN25_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNV91718.1"
                     /translation="MAIAAVLRTLLLLACLLFAHASPGADAGTAAPALDLPAPGQLHV
                     YVREGCPHCAEAKRFLAGLADARPGLQVVLREVDRDPVARDALMAISAATGAWPPGVP
                     TFVYGGRVLVGFDDAEHVGAELLRLLDEAPPPPRDRVESGLFGTLSASELGLPMFTLA
                     LGLLDGFNPCAMWVLLFLLSLLVRLQDRRRMAMVAGTFVLASGAVYYAFMAAWLNVFL
                     LVGMTAALRVGLAVLAMLIGLINVKDFFAFRRGVSLSIPESAKPGIYARARAILKAES
                     LPASLAAVAVLAVVVNFVELLCTAGLPAIYTAVLTSQDLSPLAHYGYLGLYILAYIAD
                     DALMVGTAVLALGSGKLGERGGRMLKLLSGVVMLALGAVMLLRPEWLM"
     gene            complement(8257..8829)
                     /locus_tag="PKN25_12275"
     CDS             complement(8257..8829)
                     /locus_tag="PKN25_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91719.1"
                     /translation="MNASRLLLALAASVLVVACAPEPAPAPKVEQAAPAPEAPRGEAA
                     EARREAAEAAREAMQKAGEAARKIGEAGSAAVEAAVRTAAEKAASEFAKDASRESSDA
                     GALNQAPDEDRGGDGRSPETVRDAKEAAEQAAHRIAEATRDVAERLREVGKGALESMR
                     ARTEPTTQEAAPVEERAAVPPGGGKEPAGR"
     gene            8828..8980
                     /locus_tag="PKN25_12280"
     CDS             8828..8980
                     /locus_tag="PKN25_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91720.1"
                     /translation="MKDESGFDGDSLQGTQSRREEELQDARRNKRKNDGAHMGPIVSV
                     TPEPLT"
     gene            complement(8973..>9050)
                     /locus_tag="PKN25_12285"
     CDS             complement(8973..>9050)
                     /locus_tag="PKN25_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNV91721.1"
                     /translation="VPAFVEIGDKIEIDTRTDEYKNRVK"
BASE COUNT         1339 a         3180 c         3144 g         1387 t
ORIGIN      
        1 cgtcggcggg gatgccgggc gggcctcaag gcatcgactt gcttgttcga ccatggcgcc
       61 cgcaggcacc agcctgggcg ccgacccgca gcagctccag cagcgcgctc agtggcccgc
      121 cgggctcgcc cgcccagacc aggttcgtcc ggttcgtccg catcgcctcc ggcagttcgt
      181 ggcgttggat cgaatcgccc atgacggcat tgtcgaggac gctcgccggc atgatggcga
      241 cgccggttcc ggccgcgacg caggccacga tagcatggta ggagccgagg tcgaggaacc
      301 ggcgcggggt gacgccggcc gcggcgaacc agtcgaccag caggcgccgg tatgagcagc
      361 catgcgggaa ggcgacgacc gtctggtcgg cgagatcctg cgcaccgccg atgcgggggc
      421 cgcctcgcgc ggtgatgagg accagctctt ccgcgaagac ggccacggcc gagaggcttc
      481 ccttgtcgaa gggttcggag acgaaggcgg cgtcgatctg gtgattgtcg agtcgccgca
      541 gcagcgccgc ggtcgttccg gtctgcagct ccacgtgcgt tcggggatgc gcggcgtggt
      601 accgcgagag gatgggcggc aggcgcacgc tggccgcact ctccagcgag cccaggcgca
      661 gcgagcccgc gactaccccg ctgcagaggt cctcctcggc ttcgtcggcc agccgcaaca
      721 ggcgctcggc gtgcccgagc aggcgatgac ccgcatcggt gagcaccacg ttccgtcctt
      781 gccgccgaaa gagttgcgta cccagtcggg actcgaactg cttgatgcgg gtcgtgacgt
      841 tggaaggcac ccgattgagc cggtccgccg cacgcagcac accgccttcc accgccacac
      901 atcggaagat gtgcaggtct tccaggctga tccttctcat atcgagaacg ttatttctta
      961 aaaattcact attcgattct atccgacgag cgtctaatcc cgccatggaa aaaaatcctc
     1021 cgtcgtctct gtcgccgggc gccatcgtct tcatcggcct ccttgcgctg gccatcgcga
     1081 tgggcatcgg tcgcttcgca tttacgccgc tgatgccgct catggtgcgc gatggcagcc
     1141 tgaataccgt caccgggacg gaatgggcga cggcgaacta cgtcggctac ctgctcggtg
     1201 cgatcagcgc ctcgtggttc gcgcgcagtc cccggctggg cttgcaggtc gggttgctcg
     1261 gcgtcgccgc gaccaccctg gcgatggctt ggggtaacgc cgccttgccg tggctcggct
     1321 tggccctgcg ggcgagtgcg ggtgtcttca gcgcctgggt gctggtgtgc gccagcagtt
     1381 ggtgcctgcc cgaactggcg cggcggagtt cgacttcgct gggcggatgg atctacaccg
     1441 gggtgggcgt gggcatcgcg gccacgggtg tgctgacctg gctgggcggc gcacaggcgg
     1501 ccgcggtgct gtgggtggag ctcgggctgc tcgccgtcgt cggcgccgtc catgtcattt
     1561 ggcgtctgcg cgggcaatcc gccgcagcga gccctgccgg cacgcccgac gcgccgaagc
     1621 ccccaccgcc ggcggtcgcg agcgggaacc tgggcatcgt gctgtgctac agcgcgttcg
     1681 gcttcggcta catcattccc gcgaccttcc tgcccaccat ggcgcggcag ctggtgtccg
     1741 acccgctcgt gttcgggctc acctggccga tcttcggcgc ggcggcggcg ctgtccgtgg
     1801 ccttcgccgc gcaccgtctg cacggctggc cgcgcaggaa ggtgtgggcg atcgcccagg
     1861 gggtgatggc ggcaggcacc ctgctgccgt tgctgcagcc ggcattgtgg gtactggccg
     1921 cctcggcggt gctggtcggc ggcaccttca tggtgaccac catggcaggc cttcagctgg
     1981 cgcgcgaacg cgcgcccgcg aacccgacgg cgctgctggc ccgcatgacc accggcttcg
     2041 ctgccggaca gatcgccggt ccgctgctcg tgcgcctggt cggcgatacg cgcattgccg
     2101 gcctcgacgc gctgggcctg gcgagcgcga ccgccggcct cctgctggcg atgaccgcca
     2161 tctggctctg gcgcgacgac atcgcggccg caacgaagcc gcgatccgct tgagacgcgg
     2221 acgaacgccc gagcacgatc gcggcgtcgg attccgccgg gcccggcacc ccttgcttca
     2281 gcacgcatca cgcctgcgct gcgccgggct ttggtctatg gttccggtac gagccgctcc
     2341 ggcgccgccg gcgggctcgt actgcccaag agcccgcatg gacaagccgc ctcatcgctt
     2401 gccggtattc ctgaatctgg cacagatccg ctttccgatc ggtgcgatcg cctcgattgc
     2461 gcaccgggtg tcgggggtct tgctgttcat cgccctgccc gtcctcgcgg cgctgctcca
     2521 cgcctcgctg cgcagcgaag cggatttcgc ctcggtgcgc ggtctgctct cgtcgccgct
     2581 ctcgctcgtg gtggccggtg tgctggcgtg ggcgctcgcg catcacgtgc tggccgggat
     2641 ccgccacctg ttgatggacg tcggcatcgg cagcgagctc gagcgtgcgc gcgccagcgc
     2701 acgcctcatc ctggtcgccg cgcccgcgct ggccctgctc cttctcgtgt gggggctgtc
     2761 atgaggttgt tcacagggca acgggcatgg gtggtgcaac gcctcagcgc cttgctgttg
     2821 ctggtgctga tcctgctcgc catgctcgcg ctgcctttcg cgtcgccgct cgatttctcc
     2881 gcgtggcgcg ccttggccgg cggcgcgctg ggggggccgc tgatcgcgct gctgttcggc
     2941 gccttgtgcg cgcacgcctg ggtaggcatg cgcgacatcg tgctcgacta cctccagccg
     3001 cgcggactgc ggctggcggt gctgggcctg atcacggtcg tgctgctcgg cgtgctggca
     3061 cgcgtgctgc tgagcctggc catggtggcg ctcgccgcct gagcggcgca ggggcggaca
     3121 tgagactgaa catctatcgc tacgacccgg agcgcggcga tccgccgcgg atgcaggcct
     3181 acgatgtgca gccggaggcg ggcgacaaga agctgctcga cctgctgatg aggctgcgcg
     3241 tgcaggacga cagcctgtcg ttccggcgct cgtgccgcga gggagtgtgc ggctcggacg
     3301 cgatgaacat caacggccgc aacggcctcg cctgcctgac cgcgctcgac gggctgaagg
     3361 agccgatcgt gctgcgcccg ctacccggct ttccggtaat ccgcgacctc gtcgtagaca
     3421 tgacgcagtt cttcgcccac taccactcga tccggccctg gctgatcaac gacgagcccc
     3481 tgcccgagcg cgagcggctg cagaccccgc ccgagcgcga gcgcctggac ggcctgtacg
     3541 aatgcatcct gtgcgcctgc tgcagcgcgt tctgcccgtc ctactggtgg aatccggaca
     3601 agttcatcgg gccgtcgggc ctgctgcagg cctatcgctt catcgccgac agccgcgaca
     3661 ccgcctccgc cgagcggctg gacttcctcg acgacgtcta tcgcctctac cgctgccgca
     3721 cgatcatgaa ctgcaccgag gtctgcccga agggactgag cccctcgcac gccatcgaac
     3781 ggatccgcct ggccttgctg cagcgttcat cctgaaaagg cagggcggga gcgcgccatt
     3841 cgccccgggg gccggaccgc gttctttccg cgtcaaagcg ccgggaggcc cgggacgagg
     3901 aacaccgcca tcgttgcggc ccaggccagc acaccgcagt ggatcgcgac gatgccgatc
     3961 tccagggcca cgatctcttc acgaaccccg agcatgcagc ggacaccccg cttcagcagc
     4021 gcgaccgaag cgccgatcgc cagcaccggc agtaccagga cggcgagcgt cccgtcctgc
     4081 aggagcgcga acgacgatag ccagagcggg agcagggata ccgtcgcgag cagacaagcg
     4141 tcgcgtgcgc tcgcccgcac cccatgggtc gccgcgacgg cgccgatcag ccagcggatc
     4201 atcaggaagc cgacgagctg cgcggcgaac agcgcgaact ccacgaactg ccaggcggaa
     4261 gcggagaggg cgggcagata gtgggccgat ccgacatcgg tcgcatggga caacatcgcg
     4321 gggggcaaca tggcggccgg cagggcgacc ccgaagacca gggtggccag cgagggcggg
     4381 cggcgggaga gttcggtcca ggcggcgtcc gaggaccaca gcatgggcgg cagatggagg
     4441 gaagacatga tcgatctctg ctggcgtgtt tgggcggctc gaggaattta tatcacaaca
     4501 tgatataaac tagcatgacc cgccccccgg cagcgaatac gttgcgacca gcgcgcggag
     4561 aacccgcccg cagctttgcg cggccacgaa gcacaggcag accgtcatcc ggtggcgcag
     4621 cgcgccccga ctatcgccgc tcccggccgg cgggcgggcg cacgatcgtg atcggcgcga
     4681 tggcgcgcgc cgccgcttgc aggccgagtg cctcgtgcag ccgtgcgtcg ctgccgacga
     4741 ggtcggcaag cgaaacgcgg tcgagtgcag ccaggaaggc ctgcagggca ccgtccagcg
     4801 cgcccttgag ccggcacgcc ggatccagca ggcagtgcga ctgcgttccg aagcactcga
     4861 cgagcgcgag gctcggttcg agctggcgga ccacgttgcc gagaccgatc tcgctgcttg
     4921 cacgcgcgag cctcaaaccc cctcccttgc cgcgtgcgcc gtcgacgaag cccttcgcca
     4981 ccagttcggt gacgatcttc atgaggtggc tgcgcgagat gtcgaaacgc tcggcgacct
     5041 ccttgatggt gatgcgccgc tcggggatgg ccccgagcta catgagtacc cggagcgcgt
     5101 aatcggtgtg ttgcgtgatg ttcatcggca gggcgtgcag aggagtcgtt gtcggctcca
     5161 tgctagcaac aatagattca tacaggttga ctcttcaagg aggaacggcc atggcccacg
     5221 tccttaccga agcctgcatc cgctgcaaat acacggactg cgtaagcatg tgcccagtcg
     5281 agacccatcc ggcgctgccc gccaccgccc cctggcacgc tcgcagcccg cctgccctcg
     5341 cctgtcgcta cactgcccgc acgaaaaccc gcgaagcgaa gggagacagg tcatggccga
     5401 gcacgctgcc gagccgacgg tgaagcattt ccgcctcgcc ctgctgtggc cgctgcggct
     5461 gctgaccgcg acgggcgcgc tggggccgca gcagcggccg tggcaggtgc tgcgcgacct
     5521 gggcgaggcc tcgccgtggc gcgaggtcga gcacgacctg gaggtcgaag gcagcgtctt
     5581 ccaggagcgg cactacaacg agttcgtgac cttcctgccc tacgtgcagc agttcctcta
     5641 cggcgagggc cgtgcgcagc gcggcgactc ggcggacgcg gggccggcga tgcgcgtgtt
     5701 ccgtcgccgc gatatcgcga aactgcgcgt ggccgcacgc gacggcgacg cgccggtgac
     5761 gctcgacgtc gtccatgtgc atctgtattt cttcttcgac gtcgatgtgg tgatcctcaa
     5821 cgtcgaggtc gccggcggcg acctgccctt gtccgcggtg caggagctga tgtatcgcat
     5881 cggccgcgcc tacccgtcgg gctgggacga gcacggccgc gcgctgcaca gcctcgccgt
     5941 cgccgagctg atcgggcgcg acgggtcggt gctggcgcgc accgacgcgc acgagcgcga
     6001 ggccttcctg acccacgtca gccgccatcg cgcaccgcgc atcgccgcgc actgggcctg
     6061 gctgatgcag ccgctggcga gcgcctactc ggcccagccc ggcacgctgc gctatcgcca
     6121 gatcgagtat caccgcatgc cgctgacggc ctatctcgcc ctcgacgacc cgcaggcgct
     6181 gacgcgcagg gatttcgtcc gcctcggcct ggtgaccggc gcgggaccgc acacggcaca
     6241 agccgaacgc gagggcggcg gcaacgccct gccctacgcc gaaccgcatc tggacgactt
     6301 cgagcgccgc cactgctacg accgcttctg gtccgcgagc ggcgcggcgc cctggacccg
     6361 ctacctgtgc agcggccacg cgctggtggt ggtgggcgat gcgaagtcgg cctatttcac
     6421 cgaccgcgag cgcggcgtgc tggcgcagtt ccgccaccag cacttcctgc tgttcctgat
     6481 cgcgcacttc cagaagtcgg cgctgctgat gttctccgag cgcctggtcg aggcgctcaa
     6541 gcgcctgcac gtcaccgacc cggagagcgt gcggcgcttc aagcgcgcga tccgcgccag
     6601 cttcgagggc ttcctgcgtt tcacgcatcg ctactggttc cacgacatcg ccgagcaggc
     6661 gcagacacgc gcgctgttcc gcatgaccag cgcgcatctg ggcaccgacg cgctgtacgc
     6721 cgagatcaag gagcgcaccg cggacatgaa cggctacctc gaggccgaca gcctgcgccg
     6781 ccaggccaac accgtggtgc gcctgaccgt ggtgacgatc ttcggcctca tcggcaccat
     6841 caccaccggc ttcctgggca tgaacctgct cgccgaggcg gaatcgccgc tcgccgagcg
     6901 cctggcctac ttcgcgctgg tgttcggcgt gtcgacggcg ctcacgctgt acacgatggt
     6961 caagtccaag cgcctgtcgg acttcctcga cgcggtgtcg gacgagcgcc tgtcgacctg
     7021 gaacaagctc cgcgcgctcg ccgccgtgtg gcgtcgcggc gcggactgaa cggccccgct
     7081 acatcagcca ctccggccgc agcagcatca ccgcaccgag cgccagcatc accacgcccg
     7141 aaagcagctt gagcatgcgg ccgccgcgct cgccgagctt gccgctgccg agcgcgagca
     7201 ccgcggtgcc gaccatcagc gcgtcgtcgg cgatgtaggc gaggatgtag aggccgaggt
     7261 agccgtagtg ggcgagcggc gagaggtcct ggctggtgag caccgcggtg tagatcgccg
     7321 gcaggccggc ggtgcatagc agctcgacga agttcaccac caccgccagc accgcgaccg
     7381 cggcgagcga ggcgggcagg gattcggcct tcaggatggc gcgcgcccgc gcgtagatgc
     7441 ccggcttggc ggactccggg atggacagcg agacgccacg acggaaggcg aagaagtcct
     7501 tcacgttgat caggccgatg agcatggcca gcacggcgag gccgacgcgc agcgcggccg
     7561 tcatgccgac gagcaggaag acgttgagcc aggccgccat gaaggcgtag tacaccgcgc
     7621 cgctggcgag cacgaaggtg ccggcgacca tcgccatgcg gcggcggtcc tgcagcctca
     7681 ccagcagcga cagcaggaac agcagcaccc acatcgcgca ggggttgaag ccgtcgagca
     7741 agccgagcgc cagggtgaac atcggcaggc cgagctcgct cgcgctcagg gtgccgaaca
     7801 ggcccgactc cacccggtcg cgcggcggcg gcggggcctc gtcgagcagg cgcagcagct
     7861 cggcgccgac gtgctcggcg tcgtcgaagc cgaccagcac ccggccgcca tagacgaagg
     7921 tgggcacgcc cggcggccac gccccggtcg ccgcgctgat cgccatcagg gcgtcgcgcg
     7981 cgacggggtc gcgatcgacc tcgcgcagca cgacctgcag cccggggcgg gcgtcggcga
     8041 gaccggcgag aaagcgcttg gcctccgcgc aatgcgggca gccttcgcgc acatagacgt
     8101 ggagctggcc cggcgcgggg agatcgagcg ccggcgcggc ggtgccggca tcggcgcccg
     8161 ggctcgcatg ggcgaacagc aggcatgcca gcagcaacag ggtacgaaga accgccgcga
     8221 tcgccatgga tgcgcccctc gccggcgccg tgccgctcag cggccggcgg gctccttgcc
     8281 gcctccgggc ggaacggcgg cgcgctcctc gaccggcgcg gcctcctgcg tcgtcggctc
     8341 ggtgcgtgcg cgcatgctct cgagcgcccc cttgccgacc tcgcgcaggc gctccgcgac
     8401 gtcgcgggtg gcctcggcga tgcggtgcgc cgcctgctcg gcggcttcct tggcgtcgcg
     8461 cacggtctcg ggcgagcggc catcaccgcc acggtcttcg tcgggcgcct gattcagcgc
     8521 tcccgcgtcg gatgattcgc gcgaagcgtc cttggcgaat tcgcttgcgg ccttctccgc
     8581 cgccgtgcgc accgccgctt ccaccgcggc gctgcccgcc tcgccgatct tgcgcgcggc
     8641 ctcgccggcc ttctgcatcg cctcgcgtgc cgcctcggcg gcttcgcgcc gggcctcggc
     8701 agcctcgccg cgcggcgcct cgggcgccgg agcggcttgt tcgaccttgg gcgccggcgc
     8761 gggctcgggt gcgcaggcga cgacgagcac ggaggcggcg agggcgagga gcaggcggga
     8821 cgcgttcatg aaggacgaga gcggattcga cggcgacagc ctgcagggta cgcaatcccg
     8881 tcgcgaagaa gaactgcaag atgcacgccg caacaagcgg aaaaacgatg gggcccacat
     8941 gggccccatc gtatccgtca caccggaacc gcttacttga cgcggttctt gtactcgtcg
     9001 gtgcgggtgt cgatctcgat cttgtcgccg atctcgacga aggccggcac
//
LOCUS       DAOQBS010000123         8969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_7219_length_8969_cov_19.388602, whole genome shotgun sequence.
ACCESSION   DAOQBS010000123 DAOQBS010000000
VERSION     DAOQBS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7219_length_8969_cov_19.388602"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKN25_12290"
     CDS             complement(<1..605)
                     /locus_tag="PKN25_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNV91722.1"
                     /translation="MSDQTQTTPQPQDENHLIAERREKLAAWRASGGAFPNDFSRENT
                     AGKLDELYGDKDAEALEATPVEVRVAGRIMLKRIMGKASFVTIQDLSGRIQLYVQRDG
                     VGEDVYAQFKTWDIGDIVGCVGTVFKTKTGELTVKAAEIRLLTKSLRPLPDKFHGLTD
                     VEQKYRQRYVDLIMNEQSRFTFVARSRMVQSIRNYMTGHGFL"
     gene            932..2728
                     /locus_tag="PKN25_12295"
     CDS             932..2728
                     /locus_tag="PKN25_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV91723.1"
                     /translation="MSTFRQMYEGIEKTFWNSLTKKLFSFLLLFAINLGYLVIWQDRR
                     EAVEKALAKGTVAPEVLSSVVATFDAGLYAMLALSALALAMLLGQILYLRHLVVRPIR
                     VITAIFDEIARGEGDFSRDLPLTTHDELRELAASYNRFAGKMREIIGKVRSMSVNIAR
                     EAVQVKLGVENSARDAQRQGDMTDTVFEASTRSTEAIGEVSVSARAIDASTLRNLEIA
                     RESLGEVQNVAAKILAVSEKLQRFNHTVDELSQRSESVKEIAALIREIADQTNLLALN
                     AAIEAARAGEAGRGFAVVADEVRKLAERVNKATQEIVGNINGMLELVGDTRAENDVIN
                     DDVEQTRDVVTRSATQFEHMVGEFERTGGQLSQIAAGMASLTETNAHVHANVTDIHRL
                     SAEVAGRMENCEQRTLALTKSTEAVQELVSRFKIGTGVFDLLVNETRGFRDGVQAELE
                     QMAAARVDVFDRTYRPVPGSNPPKFRVAWGEEFTRRCQRLLDESLERARGAVFAVAVN
                     TDGYLSAHNSRYSQPLTGDPAKDLVGNRTCRKFENPAELRAARNTEPMLLQTYLRDTG
                     EVLCDLAMPIVIGGRHWGNVRVGMPAEALLER"
     gene            complement(2836..5628)
                     /locus_tag="PKN25_12300"
     CDS             complement(2836..5628)
                     /locus_tag="PKN25_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV91724.1"
                     /translation="MFHIKTLELVHWDFWRRFTLPLDAQIITIVGPNGSGKTTLLDAL
                     RTLFALRCSGKRDFRRYVRRADRSFAWIRAVVANQPGHTGKRPFFPCLADEVTLACRI
                     RKAGGDWIRDYTIVDGNQPIEALEATNDWIGLRDYQHRLAWGGLTPAITKVLALEQGD
                     TDKLCEYSPKALLELVFDVFGDKEVLDNYQAAREEQKSAERELGELGIDLDRLKTQAE
                     EKKAEANRFLEWKQLADEIHALEAGIVPRLEIAELERALAAERETLFRVGHERADKLV
                     EQRESRQRLEIVRAEQESVQAERKRLKEDDRASEQRYLAAHDRVRDLERLLEERDMLR
                     AQLASEHGADAVALEKEHEEADAALAALRRKERELVAAFEERSETLRGERNRAGAPGD
                     AEVARFRVRLSAEGIAHRSLAEVVEVTDPAWQAALEAILRPYRHLVLLEREADRHAAW
                     ALGERERFRHFIVAERESAPPPRPMSLAEVVEFGAPVPRWLADLLNRIRRVDDAEAAR
                     DLPREQEWITRDGYHRERRGARHLGRPQEFHFGELARQARIAALQDEIGGVDKQLQAL
                     RPRLDAASARLTTLRQRLLGLQSAQLLAARAEAYASAEAELPAARKALTEAIAERSDT
                     RGEIDRLAEKANGIQIELDRRTRELKAIDLRLADIAREHGPRRRAQAERILALRRRRR
                     GMPAHWLDAGELALLAERYGDARGARLQLDRLRRHLDEGEWITDPAVLVVRDRLGADV
                     ALRERDYLNRQGYCTTARLHSDNARAAYIAKLRATVRQYAKNLKALGELANVSVDCPT
                     PHLENDDLSLAQAGLEVRFDFDRKGAVGLNDGEASGGQQVMKSLILLIGLLMDESRPG
                     GFVFIDEPFAHLDVANIDRVGTFLRATRAQYLITTPVTHNANVFAPAQLTLVTRKKQP
                     GTDWAPPIGVLQRAVD"
     gene            complement(5650..6756)
                     /gene="trmA"
                     /locus_tag="PKN25_12305"
     CDS             complement(5650..6756)
                     /gene="trmA"
                     /locus_tag="PKN25_12305"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - S-adenosylmethionine-dependent
                     tRNA (m5U54) methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HNV91725.1"
                     /translation="MPLPCIDPARYEDQLAAKLARYRSDFAEFDLPEPAVFRSAPLHY
                     RLRAEFRLWHHEGRIDYAMFDSADPKQPILLETFPAAAEPIAKLMPRLRDALQASEAL
                     RRKIFQVDFLATLSGECLVSLVYHRPLDADWETAARALAAALDIQVIGRSRKQKIVLE
                     RDWVQEVLEVDGQRLRYQQFEGSFTQPNGGVNQHMLAWARAQAKAVGARPQGPGDLLE
                     LYCGNGNFTVALAPLFGRVLATEMSKTSVRAAHTNLADNGVANVTMVRMASEEISDAL
                     AGGREYRRMQGVDLAGYRFSTLFVDPPRAGLDEGTIALAKGFEHILYISCNPETLRAN
                     VAALATTHRIAAAAAFDQFPYTHHLECGLLLQRR"
     gene            complement(6761..7471)
                     /locus_tag="PKN25_12310"
     CDS             complement(6761..7471)
                     /locus_tag="PKN25_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91726.1"
                     /translation="MEHELKTLTARLLAQRWLPRGDKLVRRALVDETFRLELDRRLAD
                     VGLRLLDNPYAAHVGVALQPELAEPVFGAGREYAASNLGLTRDEVALLVILWALIVLP
                     KRQRQVTRRELVDDGQTDLFGGDKPVAHGDAVSGTLTEASLIADFGPRLGGRTKVRNF
                     MLGKLARLGFIERRNGLVTEGPLLDLALDYRVMADRIIHGTLGEILEAAGHAPPESNA
                     FELAEVLPDEAEALDEED"
     gene            complement(7505..8788)
                     /locus_tag="PKN25_12315"
     CDS             complement(7505..8788)
                     /locus_tag="PKN25_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91727.1"
                     /translation="MNDPFLSDFLAAGVDADEVPELAALASARPLLDAFITLFRGTET
                     EVLMRLLVLREIGREADAPRWAPEALRARFSYLDPVKLETVLKRLRENGLLAIGEDGH
                     YALSDHGRNAVAAIAMLLRFGEEEDAELGFLTAQLAGLQAVGGITAESLGHLLSKLND
                     LTWHFEEAIASGSEFRILDARRRLSANGRWLERGTELLDKLLADPEVDFDIARIAQRI
                     GLAQSRLARADASFQRALNKIEAQRVTLGASGISSSDVAAWLRGLDAAALATLAADAC
                     ASVPELPLLAGGHELLDRAESLLEGGGSAAPADTTLPPADDAATGFAPQAEDLRMLEH
                     FTHRLGALPAPQPLHHVIAGGGFAPASYRLSLLALLADGAETAPEGGPEDGPVSSFMR
                     LPVEVEFGDTLTAVGEDEIGAMTLGEVRPRATTAA"
     gene            complement(8807..>8969)
                     /gene="prfB"
                     /locus_tag="PKN25_12320"
     CDS             complement(8807..>8969)
                     /gene="prfB"
                     /locus_tag="PKN25_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239085.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNV91728.1"
                     /translation="SKSDIGWGHQIRSYVLDQSRIKDLRTNYEVGNTQAVLDGDLDDF
                     IGASLKQGV"
BASE COUNT         1245 a         2975 c         3370 g         1379 t
ORIGIN      
        1 aggaagccgt ggccggtcat gtagttgcgg atcgactgca ccatgcggct gcgcgccacg
       61 aaggtgaagc gcgactgctc gttcatgatc aggtcgacat agcgctggcg gtacttctgc
      121 tcgacgtcgg tgaggccgtg gaacttgtcg ggcagcgggc gcaggctctt ggtgagcagg
      181 cggatctccg cggccttgac ggtgagttcg ccggtcttgg tcttgaacac cgtgcccacg
      241 cagccgacga tgtcgccgat gtcccaggtc ttgaactggg catagacgtc ctcgcccacg
      301 ccgtcgcgct gcacgtagag ctggatgcgg ccggagaggt cctggatggt gacgaagctc
      361 gccttgccca tgatgcgctt caacatgatg cggccggcca ccctgacctc gaccggcgtc
      421 gcttccagcg cctcggcgtc cttgtcgccg tagagctcgt cgagcttgcc ggcggtgttc
      481 tcgcgcgaga aatcgttggg gaaggcaccg ccgctcgcgc gccaggcggc gagtttctcg
      541 cggcgctcgg cgatgaggtg gttttcgtcc tggggctggg gcgtggtctg cgtctggtcg
      601 gacatgatct gggtgtcgaa gctctgaaag gggatgcgcc gcagcgggcc gcgtgcgaaa
      661 ggcgtgattg tggcagggcc ggcaaggcgg ggcaattgcg caacgcgccc cggacccacg
      721 gaatcttctg gagatgtccg cgaatccgcg gtcccgcccc aggatcgtcc ccgcttcgag
      781 agtctccggc aggcgcgacg caagtcgcga tcggctgctg cggaggcgtc catccgcagc
      841 atgagtcggt gatctcccgg gcccgatcct aaagtcccca ttgcggggac cgataagaca
      901 gaggtcgatt caaactcccc gcggaacccc catgagcacc ttccggcaaa tgtacgaagg
      961 catcgagaag accttctgga actcgctgac caagaaactc ttcagcttcc tgctgttgtt
     1021 cgcgatcaac ctgggctatc tcgtcatctg gcaggaccgg cgcgaggcgg tggagaaggc
     1081 gcttgccaag ggtacggtgg cgccggaggt cctctcgtcg gtggtcgcga ccttcgacgc
     1141 cggcctctac gcgatgcttg cgctctcggc cttggcgctg gcgatgctgc tcggccagat
     1201 cctctatctg cgccacctgg tcgtgcgccc gatccgcgtc atcaccgcca tcttcgacga
     1261 gatcgcgcgc ggcgagggcg acttctcccg cgacctcccg ctcaccacgc acgacgagct
     1321 gcgcgagctc gcggcgagct acaaccgttt cgccggcaag atgcgcgaga tcatcggcaa
     1381 ggtgcgctcg atgagcgtca acatcgcacg cgaggcggtg caggtgaagc tgggcgtgga
     1441 gaactccgcg cgcgatgcgc agcgccaggg cgacatgacc gacaccgttt tcgaggccag
     1501 cacgcgctcg accgaggcga tcggcgaggt gtcggtgagc gcacgcgcga tcgacgcctc
     1561 gaccctgcgc aacctcgaga tcgcgcgcga gtcgctcggc gaggtgcaga acgtcgccgc
     1621 caagatcctc gcggtgagcg agaagctgca gcgcttcaac cacacggtgg acgagctctc
     1681 gcagcgctcg gagagcgtca aggagatcgc cgcgctgatt cgcgagatcg ccgaccagac
     1741 caacctgctc gcgctcaatg ctgcgatcga ggctgcacgc gcaggcgagg ccgggcgcgg
     1801 cttcgcggtc gtcgccgacg aggtgcgcaa gctcgccgag cgggtcaaca aggccaccca
     1861 ggagatcgtc ggcaacatca acggcatgct cgagctggtg ggcgacacgc gcgccgagaa
     1921 cgacgtgatc aacgacgacg tcgagcagac gcgcgacgtg gtgacgcgct cggccacgca
     1981 gttcgagcac atggtgggcg agttcgagcg caccggcggg cagctctcgc agatcgccgc
     2041 cggcatggcc tcgctgaccg agaccaacgc ccatgtgcac gccaacgtca ccgacatcca
     2101 ccgtctgtcc gccgaggtgg ccgggcgcat ggagaactgc gagcagcgca ccctcgcgct
     2161 caccaagtcg accgaggcgg tgcaggagct ggtgtcgcgc ttcaagatcg gcaccggggt
     2221 cttcgacctg ctggtcaacg aaacgcgcgg tttccgtgac ggcgtccagg ctgaactcga
     2281 gcagatggcg gctgcgaggg tggacgtctt cgatcgcacc taccgcccgg tgccgggcag
     2341 caacccgccc aagttccgcg tggcctgggg cgaggaattc acccgccgct gccagcgcct
     2401 cctcgacgag agcctggaac gcgcgcgcgg cgcggtgttc gcggtcgcgg tcaataccga
     2461 cggttacctc tccgcgcaca acagccgcta ctcgcagccg ctgaccggcg atcccgccaa
     2521 ggatctggtc ggcaaccgca cctgccgcaa gttcgagaac cccgccgagc tgcgcgccgc
     2581 gcgcaacacc gagccgatgc tgctgcagac ctacctgcgc gacaccggcg aggtgctgtg
     2641 cgacctcgcc atgccgatcg tgatcggcgg gcgccactgg ggcaacgtgc gcgtcggcat
     2701 gccggccgag gccctgctgg agcgctgaac gagcgccgtg ttcgcgcctg gcggcgctcc
     2761 tgcgtgaaac agcgccccct gcaggagcgg cgcaagccgc gaacgcggcc gcagggtgct
     2821 cgacggcctt gtccttcaat ccaccgcgcg ctgcagcacc ccgatcggcg gcgcccagtc
     2881 ggtgccgggc tgcttcttgc gcgtgaccag cgtgagctgg gcgggcgcga agacgttggc
     2941 gttgtgggtc accggcgtgg tgatcagata ctgcgcgcgg gtggcgcgca ggaaggtgcc
     3001 gacgcggtcg atgttggcga cgtccagatg tgcgaagggc tcgtcgatga agacgaagcc
     3061 gccggggcgg gactcgtcca tcagcaggcc gatcagcagg atcagcgact tcatgacctg
     3121 ctggccgccc gaggcctcgc cgtcgttgag gcccaccgcg cccttgcggt cgaagtcgaa
     3181 gcgcacctcg agcccggcct gggcgagcga gaggtcatcg ttctccaggt gcggggtggg
     3241 gcagtcgacg ctgacgttgg cgagctcgcc gagcgccttc aggttcttcg cgtactggcg
     3301 cacggtggcg cgcagcttgg cgatgtaggc ggcgcgggcg ttgtcggagt gcagccgcgc
     3361 ggtggtgcag tagccctggc ggttgaggta gtcgcgctcg cgcagggcga cgtcggcgcc
     3421 gagacggtcg cgcaccacca gcacggcggg gtcggtgatc cactcgccct cgtcgaggtg
     3481 gcggcgcagg cggtcgagct gcaggcgggc gccgcgggcg tcgccgtatc tttcggcgag
     3541 cagggccagc tcgccggcgt ccagccagtg cgccggcatg ccgcgccggc gccggcgcag
     3601 ggcgaggatg cgctcggcct gggcgcggcg gcgcgggccg tgctcgcgcg cgatgtcggc
     3661 caggcgcagg tcgatcgcct tgagttcgcg ggtgcggcgg tcgagctcga tctggatgcc
     3721 gttcgccttc tcggcgaggc ggtcgatctc gccgcgcgtg tcgctgcgct cggcgatcgc
     3781 ctcggtgagc gccttgcgcg cggcgggcag ctcggcctcc gcgctggcgt aggcctcggc
     3841 gcgggcggcg agcagctgcg ccgactgcag gccgagcagg cgctggcgca gcgtggtgag
     3901 gcgggcgctc gcggcgtcga gcctggggcg cagggcctgg agctgcttgt ccaccccgcc
     3961 gatctcgtcc tgcagcgcgg cgatgcgcgc ctggcgggcg agctcgccga agtggaactc
     4021 ctgcggccgg ccgaggtggc gggcgccgcg gcgctcgcgg tggtagccgt cgcgggtgat
     4081 ccactcctgc tcgcgcggca gatcgcgcgc ggcctcggcg tcgtcgacgc ggcggatgcg
     4141 gttcaacagg tcggcgagcc agcgcggcac cggcgcgccg aattccacca cctcggcgag
     4201 gctcatcggg cgcggcggag gtgcgctctc gcgctcggcc acgatgaagt ggcggaagcg
     4261 ctcgcgctcg ccgagcgccc aggcggcgtg gcggtcggct tcgcgttcga gcaggacgag
     4321 gtggcggtag gggcgcagga tggcttcgag cgcggcctgc caggcggggt cggtgacctc
     4381 caccacctcg gcgaggctgc ggtgggcgat gccctcggcg ctcagccgga cgcggaagcg
     4441 cgcgacctcg gcgtcccccg gcgcgccggc gcggttacgc tcgccgcgca gggtctcgct
     4501 gcgctcctcg aaggcggcga cgagctcgcg ctccttgcgg cgcagcgcgg cgagcgcggc
     4561 gtcggcctcc tcgtgctcct tctccagcgc cacggcgtcg gcgccgtgct cgctggcgag
     4621 ctgggcgcgc agcatgtcgc gctcctcgag cagcctttcg aggtcgcgca cgcggtcgtg
     4681 ggcggcgagg tagcgctgtt cggaggcgcg gtcgtcctcc ttcaggcgct tgcgctcggc
     4741 ctgcacggac tcctgctcgg cgcgcacgat ctccaggcgc tggcgcgatt cgcgctgctc
     4801 gacgagcttg tcggcgcgct cgtggccgac gcggaacaag gtctcgcgct cggcggcgag
     4861 cgcgcgctcg agctcggcga tctccaggcg cggcacgatc ccggcctcga gcgcgtggat
     4921 ctcgtcggcg agctgtttcc attccaggaa gcggttggcc tcggccttct tctcctcggc
     4981 ctgggtcttc aggcgatcga ggtcgatgcc gagctcgccg agctcgcgct cggccgattt
     5041 ctgctcctcg cgcgcggcct ggtagttgtc caggacctcc ttgtcgccga agacgtcgaa
     5101 gacgagctcg agcaaggcct tgggcgagta ttcgcacagc ttgtcggtgt cgccctgctc
     5161 gagcgccagc accttggtga tcgccggggt gaggccgccc caggccaggc ggtgctggta
     5221 gtcgcgcagg ccgatccagt cgttggtggc ttccagcgcc tcgatcggct ggttgccgtc
     5281 gacgatggtg tagtcgcgga tccagtcgcc gccggccttg cggatgcggc aggcgagcgt
     5341 gacctcgtcg gccaggcagg ggaagaaggg acgcttgccg gtgtggccgg gctggttggc
     5401 gaccaccgcg cggatccacg cgaagctgcg gtcggcgcgg cgcacgtagc ggcggaaatc
     5461 gcgcttgccc gagcagcgca gcgcgaacag cgtgcgcagg gcgtcgagca gcgtggtctt
     5521 gcccgagccg ttggggccga cgatggtgat gatctgggca tccagcggca gcgtgaagcg
     5581 ccgccagaag tcccagtgga cgagctcgag ggtcttgatg tggaacatgg gccggggtcg
     5641 cggtgaggct cagcgccgct gcagcagcag cccgcattcg aggtggtggg tgtaggggaa
     5701 ctggtcgaag gccgcggccg cggcgatgcg gtgcgtcgtc gccagcgcgg cgacgttggc
     5761 gcgcagggtc tccgggttgc aggagatgta gaggatgtgc tcgaagccct tcgccagcgc
     5821 gatcgtgcct tcgtcgaggc cggcgcgcgg cgggtccacg aagagcgtgg agaagcggta
     5881 gccggccagg tccacgccct gcatgcggcg gtattcgcgc ccgccggcga gggcgtcgct
     5941 gatctcctcg ctcgccatgc gcaccatggt gacgttggcg acgccgttgt cggcgaggtt
     6001 ggtgtgcgcg gcgcgcaccg aggtcttgct catctcggtc gcgagcacgc ggccgaacag
     6061 cggcgccagc gccacggtga agttgccgtt gccgcagtag agttcgagca ggtcacccgg
     6121 gccttgcgga cgcgcgccca cggccttcgc ctgcgcgcgc gcccaggcca gcatgtgctg
     6181 gttcacgccg ccgttgggct gggtgaagct gccctcgaac tgctggtagc gcagacgctg
     6241 gccgtcgacc tcgagcacct cctgtaccca gtcgcgctcg agcacgatct tctgcttgcg
     6301 gctgcgcccg atcacctgga tgtcgagcgc ggcggcgagc gcgcgcgcgg cggtttccca
     6361 gtccgcgtcg agcggacggt ggtagaccag gctgaccagg cactcgccgc tcagggtggc
     6421 gaggaagtcg acctggaaga tcttgcgccg cagcgcctcg ctggcctgga gcgcgtcgcg
     6481 caggcgcggc atcagcttgg cgatcggttc tgcagccgcg gggaaggtct cgagcaggat
     6541 gggctgcttc gggtccgcgc tgtcgaacat cgcgtagtcg atgcggccct cgtgatgcca
     6601 caggcggaac tcggcgcgca ggcggtagtg cagcggcgcg gagcggaaga ccgccggctc
     6661 gggcaggtcg aactcggcga agtcgctgcg gtagcgcgcg agcttggcgg cgagctggtc
     6721 ctcgtagcgc gcggggtcga tgcagggcag gggcatgggc tcagtcttcc tcgtcgaggg
     6781 cttcagcttc gtcgggcagg acctcggcga gttcgaaggc gttgctctcc ggcggcgcgt
     6841 ggccggcggc ttcgaggatc tcgcccaggg tgccgtggat gatgcggtcg gccatcacgc
     6901 ggtagtcgag cgcgaggtcg agcagtggtc cctcggtgac gagcccgttg cggcgctcga
     6961 tgaagccgag gcgggcgagc ttgccgagca tgaagttgcg caccttggtg cgccctccca
     7021 ggcgcgggcc gaagtcggcg atcagggagg cctcggtgag cgtgcccgag acggcatcgc
     7081 catgcgccac cggcttgtcg ccaccgaaga gatcggtctg gccgtcgtcg acgagctcgc
     7141 gccgggtcac ctggcgctgc cgcttgggca gcacgatcag cgcccagagg atcaccagca
     7201 gcgcgacctc gtcgcgcgtc aggccgaggt tgctggccgc gtactcgcgc ccggcgccga
     7261 acaccggttc ggcgagttcg ggctgcagcg ccacgccgac gtgggcggcg taggggttgt
     7321 cgagcaggcg caggccgacg tcggccagcc gccggtcgag ttcgaggcgg aaggtttcat
     7381 ccaccagcgc gcgtcgcacc agcttgtcgc cgcgcggcag ccagcgctgg gcgagcaggc
     7441 gcgcggtgag ggtcttgagt tcgtgttcca tggaaatcct gggggctcgg gccgggggcg
     7501 gggctcaagc cgcggtggtg gcgcgcgggc gcacctcgcc gagcgtcatc gcgccgatct
     7561 cgtcctcgcc gaccgcggtc agcgtgtcgc cgaactcgac ctccaccggc aggcgcatga
     7621 agctgctcac cgggccatcc tccgggccgc cctccggagc ggtctccgcg ccgtcggcga
     7681 gcagcgcgag cagcgacagg cggtagctcg ccggggcgaa accgccgccg gcaatcacgt
     7741 ggtgcagggg ctgcggcgcg ggcagggccc cgagacgatg ggtgaagtgt tcgagcatgc
     7801 gcaggtcttc ggcctgtggg gcgaagccgg tcgcggcgtc gtcggccggg ggcagggtgg
     7861 tgtcggccgg ggccgcgctg ccgccgccct cgagcaagga ttcggcgcgg tcgagcagct
     7921 cgtgcccgcc ggcgagcagc ggcagctccg gcacgctcgc gcaggcgtcc gccgccagcg
     7981 tggcgagcgc cgcggcgtcg agcccgcgca gccaggcggc gacgtccgag gacgagatgc
     8041 ccgaggcccc cagcgtcacg cgctgcgcct cgatcttgtt cagcgcgcgc tggaaggagg
     8101 cgtcggcgcg cgccaggcgc gactgcgcca gcccgatgcg ctgcgcgatg cgggcgatgt
     8161 cgaagtcgac ctcggggtcg gcgagcagct tgtcgaggag ctcggtgccg cgctcgagcc
     8221 agcgcccgtt ggcagacagg cggcggcggg cgtcgaggat gcggaactcg gagccggagg
     8281 cgatcgcctc ctcgaagtgc caggtgaggt cgttgagctt ggacagcaga tggccgagcg
     8341 actcggcggt gatgccgccg actgcctgca ggccggcgag ctgggcggtg aggaagccga
     8401 gctcggcgtc ttcttcctcg ccgaagcgca gcagcatcgc gatcgccgcc accgcgttgc
     8461 ggccatggtc ggagagcgcg tagtggccgt cctcgccgat cgcgagcagg ccgttctcgc
     8521 gcaggcgctt caggaccgtc tcgagcttga ccgggtcgag atagctgaag cgtgcgcgca
     8581 gcgcctcggg cgcccagcgc ggcgcgtcgg cttcgcggcc gatctcgcgc agcaccagca
     8641 ggcgcatcag cacctcggtc tcggtgccgc ggaacagcgt gatgaaggcg tcgagcagcg
     8701 ggcgcgccga agccagcgcc gcgagctcgg gcacttcgtc ggcgtccacg cccgcggcga
     8761 ggaagtcgga gaggaagggg tcgttcaagc ctgcaacgcc ctttcctcag acgccctgct
     8821 tgaggcttgc cccgatgaaa tcgtcgagat cgccgtcgag cacggcctgc gtgttgccga
     8881 cctcgtagtt ggtgcgcaga tccttgatgc gcgactggtc gagcacgtag ctgcggatct
     8941 ggtgacccca gccgatgtcg ctcttggag
//
LOCUS       DAOQBS010000124         8961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_7231_length_8961_cov_17.215360, whole genome shotgun sequence.
ACCESSION   DAOQBS010000124 DAOQBS010000000
VERSION     DAOQBS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7231_length_8961_cov_17.215360"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PKN25_12325"
     CDS             complement(<1..355)
                     /locus_tag="PKN25_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNV91729.1"
                     /translation="MAAKEVKFGDSARERMVAGINILANAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDIAGDGTTTATVLAQSIVRE
                     GMKFVAAGMNPMDLKR"
     gene            complement(408..698)
                     /gene="groES"
                     /locus_tag="PKN25_12330"
     CDS             complement(408..698)
                     /gene="groES"
                     /locus_tag="PKN25_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNV91730.1"
                     /translation="MKIRPLHDRVIVKRLEAERKTASGIVIPDSAGEKPDQGEVLAVG
                     NGKILDDGKVRPMAVKVGDKVLFGKYAGQTVKVEGDELLVMREEDIMGVVEA"
     gene            964..2097
                     /locus_tag="PKN25_12335"
     CDS             964..2097
                     /locus_tag="PKN25_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNV91731.1"
                     /translation="MDARWKGAAFEGDRASLARLAAAGAKVVRVYRQSDAWVLDEAHR
                     LGLKVVMGLWLEHPRHGFDYADARAMRAQEDALLDFVARHRKHPALLAWGVGNEIETG
                     VADPLPLWRAVDRLAARIRALDPDHPTMMVVADTGMDAFRALAGCCPNVELLGINVYA
                     GAVFDLPQRLRAAGIAKPVVVAELGPLGQWQAGRKPWGAPVELTSTEKARFFTEALAF
                     LDQQAQIRGVFPFLWGAKQEQTATWHGLLLADGSPTAMSDALAAAWGRPQPRPAPRIR
                     GIGIGADEFAAGAEISAGIDAVAHDGSALAAEWAVHAEATDLRKGGDAETPPTRIDVR
                     VLHADAASVSFVAPAQPGAYRLFITVRDREGKAGTANLPFRVR"
     gene            2276..4735
                     /locus_tag="PKN25_12340"
     CDS             2276..4735
                     /locus_tag="PKN25_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV91732.1"
                     /translation="MSAPLTPPAPLPVERLATRCDPAALGIETSAQLPELDVARLHGR
                     AVDAIRLGLDIRAEGYNLFVLGDPGSGRHELVRRLLEDTRGRGDAPADWCYAWNFANA
                     AQPRLLRLPCGRGPALRDDLARFVEELVPAIGAVFESEEHRNRIEALQEEAKTREESA
                     LRSLGDEAQKLGVALLRTPHGFAFLPMKDEGSTLTQEEFEQLPEARQHELGEHIRALH
                     ERLHRLMGDFPRWRHELQNRIRDAGREAIRATVTHMVDELKARHADLPEVCAHLDAVL
                     ADVVASGESLRATPHADEDSETLTYTGSISVQRYLVNLLVANPADGTRPMVYEDHPTL
                     QNLVGRIDHLVHMGTLVSNFTLIRAGALHRANGGFLVLDALKLLSQPFAWEGLKRALK
                     SARLRIESLSELIGVTGSVQLEPEPMPLELKVVLIGDRLTYYLLGRYDPEFAALFRIN
                     ADMESEIERSADNTAAYACLLATLARRAGLPPLSAPALARLIEHAARLAADAERLSAR
                     TQPLDDRLREAAHFALAAGAARIEREHVDAAIAAHRRRHERIRLGHLDQILRGQWLID
                     TAGSHVGQVNGLAVVPLGEDSFAHPQRITATVRAGAGEVIDIEREVKLGGPIHSKGVL
                     ILSAFLAARFGWMLPLSLKASLVFEQSYGGVEGDSASLAELVALLSALSGVAVKQSLA
                     VTGSVNQFGVVQPVGGINEKIEGFFDLCAVRGLDGRQGVLIPRANVCHLMLRDDVVEA
                     VRAGRFAVWAVADADEAVELLTGVAAGVPDEQGRMAAGSMSRRVVEGLRKLARMQREF
                     ARRGHEDDADAAGHRGRPHAS"
     gene            complement(4811..5065)
                     /locus_tag="PKN25_12345"
     CDS             complement(4811..5065)
                     /locus_tag="PKN25_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNV91733.1"
                     /translation="MDDTELRSIVIATLKEIAPEIEEAGLRPERPLRQQVDLDSMDWL
                     NFLVGLAGRLAVEIPEADYRKLATVNDLVAYLRDARRRAG"
     gene            complement(5090..6304)
                     /locus_tag="PKN25_12350"
     CDS             complement(5090..6304)
                     /locus_tag="PKN25_12350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNV91734.1"
                     /translation="MFEFKLPSLGADMDEGKLLEWLVRPGDRVVKGQVVAIVDTSKAA
                     VDVEIWQDGTVHELLVEPGTRMAVGTVMATLLEPGEAPAAKRTRTKTVKAAAAAAVPV
                     VEAPAAKPAPDVSPAPAGRLRVSPAARKRAEALGVELAALSGSGPGGAVTLEDVERAV
                     AAARLPASPPAATPSPISAAERQAETRRAIAAAMSRSKREIPHYYLFETVPMARAQDW
                     LGRANAGRPITERLLMAVLQLKAVALALRKYPELNGFHRDGQFVAAQGIHVGVAISLR
                     QGGLIAPALHDVDHKPLDVLMRELADLVRRTRAGSLRSSEMGDPTVTVTNLGEQGVEA
                     VAGVIYPPQVALVGFGRIAPRPWVREDRGELCVMPVVTASLAADHRVSDGHRGALFLA
                     ELRELLQRPEEL"
     gene            complement(6297..7268)
                     /locus_tag="PKN25_12355"
     CDS             complement(6297..7268)
                     /locus_tag="PKN25_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNV91735.1"
                     /translation="MRMSYREALRLALREALQNDARVFLMGEDVGRYGGSYAVSKGLL
                     EEFGPERIRDTPLSELGFVGAGVGAALGGLRPIVEVMTVNFSLLALDQIVNSAALLRH
                     MSGGQCSVPLVLRMATGAGRQLGAQHSHSLENWYAHIPGIRVLAPATVADARGMLAPA
                     LADPDPVVIFEHAQLYNLEDELPDDWRCDIARAAVRRAGAQASVFAYGGCLPKALQAA
                     EELAAQGVEVEVVDLRVLRPLDIDTVAASVRRTHRAVVVDEGWRSGSLAAELIARIVE
                     TCFYELDAPPARVCSEEVPIPYAKHLEEAALPQVPKIVAAVKELLNV"
     gene            complement(7265..8278)
                     /gene="pdhA"
                     /locus_tag="PKN25_12360"
     CDS             complement(7265..8278)
                     /gene="pdhA"
                     /locus_tag="PKN25_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNV91736.1"
                     /translation="MTRTRKPRASAERPLELRLLADMLRIRRMEEKCAELYGAGRIRG
                     FLHLYIGEEACATGAMHALAADDNVVATYREHGHALLRGVGMDAIMAEMFGKAAGCSR
                     GRGGSMHLFDVARRFYGGNAIVGGGLPLAVGLALADRLQGVRRVTLCVFGEGAMAEGA
                     FHESINLAALWQLPVLFCCENNLYAMGTALARSESQTDLCAKAASYRVATFRADGMDV
                     RAVFDTVSRAVRQVREGGGPAFVELQTYRFRAHSMFDPDLYRDKAEIEEWKQRGPIHS
                     LSAQLKAEGRLDEEAFLALDAEAEAEVAAAVAFAEQAPWEPVADLLKDVHTPAGAAAG
                     GTP"
     gene            complement(8275..>8961)
                     /locus_tag="PKN25_12365"
     CDS             complement(8275..>8961)
                     /locus_tag="PKN25_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV91737.1"
                     /translation="PIHDNWWQTETGGIMIANLPALDIKPGSMGKPLPGVEAAIVRPR
                     EGGGVEFVDAPDEEGELALKRGWPSMLRGYLNNEERYRKCFAGDWYLTGDLARRDADG
                     YYWFVGRSDDVIKSAGHLIGPFEVESALMEHPAVAEAGVIGKPDDMVGEVVKAFVSLK
                     QGFEPSEALRKELLGHARKKLGAAVAPKEIDFLPVLPRTRSGKIMRRLLKARELGLPE
                     GDTSTLEAGA"
BASE COUNT         1271 a         3323 c         3045 g         1322 t
ORIGIN      
        1 cgcgcttgag gtccatcggg ttcatgccgg cggcgacgaa cttcatgcct tcgcgcacga
       61 tcgactgcgc cagcacggtg gcggtggtgg tgccgtcacc ggcgatgtcg gaggtcttgg
      121 aagcgacttc cttgaccatc tgcgcgccca tgttctcgaa cttgtccttc agctcgattt
      181 ccttggcgac ggagacgccg tccttggtca cggtcggggc gccgaacgag cgctcgagca
      241 cgacgttgcg gcccttcggg cccagggtca ccttgaccgc gttggcgagg atgttgatgc
      301 cggcgaccat gcgctcgcgg gcggaatcac caaacttgac ttctttagct gccattcttc
      361 agaactccgg aatcgtttcg attagggttg atcggtgcgg gggaggctca ggcctcgacc
      421 acgcccatga tgtcttcctc gcgcatcacg aggagttcgt cgccttcgac cttcacggtc
      481 tggccggcgt acttgccgaa cagcaccttg tcaccgacct tgacggccat cggacggacc
      541 ttgccgtcgt cgaggatctt gccgttgccg accgcgagga cttcgccctg atcgggcttc
      601 tcgccggcgc tgtccgggat cacgatgccg ctggcggtct tgcgttcggc ttcgaggcgc
      661 ttgacgatca cacgatcatg caggggacga atcttcatcg agttctctcc taactgaggt
      721 tcaactgtct gggttgatga aacacgcgga accagtccgc aggaaaagga tctgctgtgg
      781 cgatggacgg gcttgcaccc gtcgatcgag gcttgttagc actcgacgcc aacgagtgct
      841 aataataggg acgggtccag aggatttcaa gtgcgcttcc tgttaacttt tttctgcgcc
      901 gtgatggcgc tcgccgcggc gcccctccac gcccaaagcc aacctccccc cctcgccgcc
      961 tccatggacg cacgatggaa gggcgccgcc ttcgagggcg accgcgccag cctcgcccgc
     1021 ctcgccgccg ccggcgccaa ggtcgtgcgc gtgtaccgcc agtccgacgc ctgggtgctc
     1081 gacgaggcgc accgcctcgg cctgaaggtg gtgatggggc tgtggctgga gcacccgcgc
     1141 cacggtttcg actacgccga cgcgcgcgcc atgcgcgcac aggaagacgc cctgctcgac
     1201 ttcgtcgccc gccaccgcaa gcaccctgcc ctgctcgcct ggggcgtggg caacgagatc
     1261 gagaccgggg tcgccgaccc gctgccgctg tggcgcgcgg tcgaccggct ggccgcgcgc
     1321 atccgcgcgc tcgaccccga ccaccccacc atgatggtgg tcgccgacac cggcatggac
     1381 gccttccgcg cactcgccgg ctgctgcccc aacgtcgagc tgctcggcat caacgtctac
     1441 gccggtgcgg tgttcgacct gccgcagcgc ctgcgcgcgg ccggcatcgc caagccggtg
     1501 gtggtcgccg agctcggccc gctcgggcag tggcaggccg ggcgcaagcc ctggggcgcg
     1561 ccggtcgagc tcaccagcac cgagaaggcg cgcttcttca ccgaggccct cgccttcctc
     1621 gaccagcagg cgcagatccg cggcgtcttc cccttcctgt ggggcgcgaa gcaggaacag
     1681 accgcgacct ggcacggact gctgctcgcc gacggtagcc ccaccgcgat gagcgacgct
     1741 ctcgccgccg cctgggggcg gccgcagcct cggcccgcac cgcgcatccg cggcatcggc
     1801 atcggcgcgg acgagttcgc cgccggcgcg gagatctccg ccggcatcga cgcggtcgcc
     1861 cacgacggca gcgcgctcgc cgccgagtgg gccgtgcacg ccgaggccac cgacctgcgc
     1921 aagggcggcg atgccgagac cccgcccacg cgcatcgacg tgcgcgtgct gcacgccgat
     1981 gccgccagcg tgagcttcgt cgccccggcg cagcccggcg cctaccgcct cttcatcacc
     2041 gtgcgcgacc gcgagggcaa ggcagggacg gcgaacctgc cgttccgggt gaggtaaacg
     2101 aggcctcgcc cgcagcagcg tcgtctccct cgcgcctgcg gctctcgcac gttcgtgccc
     2161 cctcctcccg tgggcgcacg gtgcccgccc cgcgcattcc tccccgcttg gtccgccgcc
     2221 ccgccccggg gttaagatga ctcgcagaag aacctcaagc gcaggaggcg cgcccatgtc
     2281 cgctccgctc acgccgccgg cccccttgcc cgtcgagcgc ctggcgactc gctgcgaccc
     2341 ggcagcgctc ggcatcgaaa cctccgccca actgcccgag ctcgacgtcg cccgcctcca
     2401 cggccgcgcg gtcgacgcga tccggctcgg cctcgacatc cgcgccgagg gctacaacct
     2461 cttcgtgctc ggcgaccccg gcagcggccg ccacgagctc gtccgccgcc tgctcgagga
     2521 cacgcgcggc cgcggcgatg cacccgcgga ctggtgctac gcatggaact tcgccaacgc
     2581 cgcccagcct cggctgctgc gcctgccctg cgggcgcggc ccggcgctgc gcgacgatct
     2641 ggcccgcttc gtcgaggagc tggtgccggc gatcggcgcg gtgttcgaga gcgaggagca
     2701 ccgcaaccgc atcgaggccc tgcaggagga ggccaagacg cgcgaggaga gcgccttgcg
     2761 cagcctcggc gacgaggccc agaagctcgg cgtcgcgctg ctgcgcaccc cgcacggctt
     2821 cgccttcctg ccgatgaagg acgagggcag cacgctcacg caggaagaat tcgagcagct
     2881 gcccgaggca cgccagcacg agctcggcga gcacatccgc gcgctgcacg agcgcctgca
     2941 ccgcctgatg ggcgatttcc cgcgctggcg gcacgagctg cagaaccgca tccgcgacgc
     3001 cgggcgcgag gcgatccgcg ccaccgtcac ccacatggtc gacgaattga aggcgcgtca
     3061 cgcagacctg cccgaggtgt gcgcgcatct ggacgcggtg ctcgccgacg tcgtcgccag
     3121 cggcgagtcg ctgcgcgcga cgccgcacgc cgacgaggac agcgagacgc tgacctacac
     3181 cggcagcatc agcgtgcagc gctatctggt gaacctgctc gtcgccaacc ccgccgacgg
     3241 cacccgaccg atggtgtacg aagaccaccc cacgctgcag aacctggtcg gccgcatcga
     3301 ccacctggtg cacatgggca ccctggtgag caacttcacc ctgatccgcg ccggcgccct
     3361 gcaccgcgcc aacggcggct tcctggtgct cgatgcgctc aagctgctgt cccagccctt
     3421 cgcctgggag gggctcaagc gcgcgctcaa atccgcacgc ctgcgcatcg agtcgctctc
     3481 ggagctgatc ggggtgaccg gctcggtgca gctcgagccc gagccgatgc cgctcgagct
     3541 caaggtggtg ctgatcggcg atcgcctgac ctactacctg ctcggccgct acgatcccga
     3601 gttcgccgcg ctgttccgca tcaacgccga catggagagc gagatcgaac gctccgcgga
     3661 caacaccgcc gcttacgcct gcctgctcgc caccctggcg cgccgcgccg gcctgccgcc
     3721 cttgtcggcg ccggcgctgg cgcgcctgat cgagcacgcg gcgcgcctcg ccgccgacgc
     3781 cgagcgcctg agcgcgcgca cccagccgct cgacgaccgc ctgcgcgagg cggcccactt
     3841 cgccctcgcc gccggcgccg cgcgcatcga gcgcgagcat gtggacgccg cgatcgcggc
     3901 ccaccggcgc cgccacgaac gcatccgcct cggccacctc gaccagatcc tgcgcgggca
     3961 gtggctgatc gacaccgcgg gcagccacgt cggccaggtc aatggcctgg cggtggtgcc
     4021 gctcggcgaa gacagcttcg cccacccgca gcgcatcacc gccacggtgc gcgccggcgc
     4081 cggcgaggtc atcgacatcg agcgcgaggt caagctcggc gggccgatcc actccaaggg
     4141 cgtgctgatc ctgtccgcct tcctcgccgc acgcttcggc tggatgctgc cgctctcgct
     4201 caaggcgagc ctggtgttcg agcagtccta cggtggcgtc gagggcgaca gcgcctcgct
     4261 cgccgagctg gtggccctgc tctcggcgct ctcgggcgtg gcggtgaagc agtcgctcgc
     4321 cgtgaccggt tcggtgaacc agttcggcgt cgtgcagccg gtaggcggga tcaacgagaa
     4381 gatcgagggc ttcttcgacc tgtgcgcggt gcgcggtctc gacggccgcc agggcgtgct
     4441 gattccgcgc gccaacgtct gccacctgat gctgcgcgac gacgtggtcg aggcggtgcg
     4501 ggccgggcgc ttcgccgtgt gggcggtggc cgacgccgac gaggcggtgg agctgctcac
     4561 cggcgtggcg gcgggcgtgc cggacgagca gggcaggatg gctgcgggct cgatgagccg
     4621 gcgcgtcgtc gagggcctgc gcaagctggc gaggatgcag cgcgagtttg cgcggcgggg
     4681 acacgaagac gacgccgacg cggccgggca tcgaggccgg ccgcacgcat cctgacgcag
     4741 gtggcgcgcg ccgagagcgg ggtcggcacc ggccggacgt ttgcggccgt tcgggcgatc
     4801 gtcgtcgggc tcagccggct cgccggcgcg cgtcgcgcag gtacgcgacc aggtcattca
     4861 ccgtcgcgag cttgcgatag tccgcctcgg gaatctcgac cgccagccgc ccggcgaggc
     4921 cgaccaggaa gttcagccag tccatcgaat cgaggtcgac ctgctggcgc agcgggcgct
     4981 cggggcgcag cccggcctcc tcgatttccg gagcgatttc cttcagcgtt gcgatgacga
     5041 tgctgcgcag ttcggtgtcg tccatggcgg ttccgtccgg aatgggagtt cacagttcct
     5101 ccggccgctg cagcagctcg cgcagctcgg cgaggaacag cgcgccgcgg tggccgtcgg
     5161 agacgcgatg atcggcggcg aggctggcgg tgacgacggg catcacgcac agctcgccgc
     5221 ggtcctcgcg cacccagggc cgcggcgcga tgcggccgaa gccgacgagg gcgacctggg
     5281 gcggatagat cacgcccgcc accgcctcca cgccctgctc gccgagattg gtgacggtga
     5341 ccgtcggatc gcccatctcc gagctgcgca gcgaaccggc acgcgtgcgc ctgaccaggt
     5401 cggcgagctc gcgcatcagc acatccagcg gcttgtggtc cacgtcgtgc agcgccgggg
     5461 cgatcaggcc gccctggcgc agcgagatgg cgacgccgac gtggatgccc tgcgcggcga
     5521 cgaactggcc gtcgcggtgg aagccgttga gctcggggta cttgcgcagc gccaaagcaa
     5581 cggccttcag ctgcagcacc gccatcagca ggcgctctgt gatcgggcgt cccgcgttgg
     5641 cgcggccgag ccagtcctgc gcgcgcgcca tcggcacggt ttcgaacagg tagtaatgcg
     5701 ggatctcgcg cttcgagcgg ctcatcgccg cggcgatggc gcggcgggtc tcggcctggc
     5761 gctcggcagc ggagatcggg cttggcgtcg ccgcgggtgg tgaagcaggc agtcgtgcag
     5821 ccgccacggc acgctccacg tcctccagcg tgaccgcgcc gcccgggccg ctgcccgaca
     5881 gcgcggcaag ctccactccc agcgcctcgg cgcgcttgcg cgcggcgggc gagacgcgca
     5941 agcgcccggc cggcgcgggc gagacgtccg gcgcgggttt cgccgcgggc gcttcgacca
     6001 ccggcaccgc cgccgctgca gccgccttca cggtcttcgt gcgcgtcctt ttcgccgccg
     6061 gcgcctcgcc gggctccagc agcgtcgcca tcaccgtgcc gaccgccatc cgcgtccccg
     6121 gctccacgag cagttcatgc accgtgccgt cctgccagat ctcgacatcc accgccgcct
     6181 tggaggtgtc gacgatggcg acgacctgac ccttcacgac cctgtctcca gggcgcacga
     6241 gccactccag cagcttgcct tcgtccatgt cggcgcccag cgaaggcagc ttgaactcaa
     6301 acattcagca gctccttcac cgcggcgacg atcttcggca cctgaggcaa ggcggcctcc
     6361 tcgaggtgct tggcgtacgg gatcggcacc tcctcgctgc acacccgcgc gggcggcgcg
     6421 tcgagctcgt agaagcaggt ctcgacgatg cgcgcgatca gctcggcagc gaggctgccg
     6481 ctgcgccagc cttcgtccac caccaccgca cgatgcgtcc ggcgcaccga tgcggcgacg
     6541 gtgtcgatgt ccagcgggcg cagcacgcgc agatcgacca cctcgacctc gacgccctgg
     6601 gccgccagtt cttccgccgc ctgcagcgcc ttgggcaggc agccaccgta agcgaacacg
     6661 cttgcctgcg cgccggcgcg gcgcaccgcg gcgcgggcga tgtcgcagcg ccagtcgtcc
     6721 ggcaactcgt cttccaggtt gtagagctgg gcgtgctcga agatcaccac cggatcgggg
     6781 tcggcgagcg ccggcgcgag catgccgcgc gcgtcggcca cggtggccgg cgccagcacg
     6841 cggatgccgg gaatgtgggc gtaccagttc tccaggctgt gcgagtgctg cgcgccgagc
     6901 tggcgcccgg cgccggtggc catgcgcagc accagcggca ccgagcactg gccgccggac
     6961 atgtggcgca gcagggcggc gctgttgacg atctggtcca gcgccagcag gctgaaattg
     7021 accgtcatca cctcgacgat cggacgcagg ccgccgagcg ccgcccccac gccggcgccg
     7081 acgaagccga gttcggacag cggcgtgtcg cggatgcgct cggggccgaa ctcctccagc
     7141 aggcctttcg acaccgcata gctgccgccg tagcggccga cgtcctcgcc catcaggaac
     7201 acgcgcgcat cgttctgcag cgcctcgcgc agcgccagcc gcagcgcctc gcggtagctc
     7261 atcctcatgg cgtaccccct gcggccgcgc cggccggcgt gtgcacgtcc ttcagcagat
     7321 ccgccaccgg ctcccatggc gcctgttcgg cgaacgcgac cgcggcggcc acctcggctt
     7381 cggcctcggc atcgagcgcg aggaaggcct cctcgtcgag cctgccctcg gccttcagct
     7441 gcgcggagag gctgtggatg gggccgcgct gcttccactc ctcgatctcg gccttgtcgc
     7501 ggtacagatc gggatcgaac atcgagtgcg cgcggaagcg gtaggtctgc agctcgacga
     7561 aggccgggcc gcccccctcc ctcacctgcc gcaccgcgcg gctcacggtg tcgaacaccg
     7621 cgcgcacgtc catgccgtcg gcgcggaagg tggcgacccg gtagctcgcc gccttggcgc
     7681 acaggtcggt ctgcgactcc gagcgcgcca acgccgtacc catcgcgtac aggttgttct
     7741 cgcagcagaa cagcaccgga agctgccaca gcgcggcgag gttgatcgac tcgtggaagg
     7801 cgccttcggc catcgcgcct tcgccgaaca cgcacagggt gacgcggcgc acgccttgca
     7861 gcctgtccgc cagcgccagg cccacggcca gcggcaggcc gccgccgacg atggcgttgc
     7921 cgccgtagaa gcggcgcgcc acgtcgaaca ggtgcatcga gccgccgcgc ccgcgcgagc
     7981 agcccgccgc cttgccgaac atctcggcca tgatcgcgtc catgcccaca ccgcgcagca
     8041 gcgcgtggcc gtgctcgcgg taggtggcga cgacgttatc gtcggcggcg agcgcgtgca
     8101 tcgcgccggt ggcgcaggct tcctcgccga tgtagaggtg caggaaaccg cggatcctgc
     8161 cggcaccgta gagctcggcg catttctcct ccatgcggcg gatgcgcagc atgtcggcca
     8221 gcaggcgcag ctccagcgga cgctctgcag aggccctggg cttgcgtgtg cgcgtcatgc
     8281 tccggcctcc agcgtggagg tgtcgccttc gggcaggccg agttcgcgcg ccttcagcag
     8341 ccggcgcatg atcttgccgc tgcgcgtgcg cggcagcacc ggcaggaagt cgatctcctt
     8401 gggtgccacc gccgcgccga gcttcttgcg cgcatgaccg agcagctcct tgcgcagcgc
     8461 ctccgaaggc tcgaagccct gcttcagcga cacgaaggcc ttcaccacct cgccgaccat
     8521 gtcgtcgggc ttgccgatga cgccggcctc ggccaccgcc ggatgctcca tcagcgcgct
     8581 ctcgacctcg aagggcccga tcaggtggcc ggccgacttg atcacgtcgt cgctgcggcc
     8641 gacgaaccag tagtagccgt cggcatcgcg ccgcgccagg tcgccggtca ggtaccagtc
     8701 gccggcgaag cacttgcggt agcgctcctc gttgttcagg tagccgcgca gcatcgatgg
     8761 ccaaccacgc ttcagggcca gttcgccctc ctcgtcgggc gcatcgacga actccacgcc
     8821 gccgccttcg cgcggacgca cgatcgccgc ctcgacaccg ggcaagggct ttcccatcga
     8881 gccgggcttg atgtcgagcg cgggaaggtt ggcgatcatg atgccgccgg tctcggtctg
     8941 ccaccagttg tcgtggatcg g
//
LOCUS       DAOQBS010000125         8918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_7273_length_8918_cov_9.297416, whole genome shotgun sequence.
ACCESSION   DAOQBS010000125 DAOQBS010000000
VERSION     DAOQBS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8918
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7273_length_8918_cov_9.297416"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PKN25_12370"
     CDS             <1..378
                     /locus_tag="PKN25_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNV91738.1"
                     /translation="LLASYQQADNTSFVDDLDADLWQVGVVVPVGAAGNVHLGYGEVE
                     YDFEGESSKAKSIGLAYTHAMSKRTTAYVGYNYTDNDDGLTLGAAPVGTADINIDANN
                     KGTVAIEDGEKSKMFVVGVRHTF"
     gene            571..810
                     /locus_tag="PKN25_12375"
     CDS             571..810
                     /locus_tag="PKN25_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNV91739.1"
                     /translation="MEATIRDLRLHTAEVLAAADRGERVTITSRGQVRAVLMRCEENR
                     ARAHAERNPAFGLWADRQGTVDDDVRRLRQPRVLP"
     gene            810..1187
                     /locus_tag="PKN25_12380"
     CDS             810..1187
                     /locus_tag="PKN25_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV91740.1"
                     /translation="MLVDTDVLIWNLRGNARAADRLDAMPGFLVSAVTYMELVQGVRD
                     KSELRALRQALSFWSARVVQIDEAISARACFLCEQFALSHSMQMADALIAATALERGL
                     DLLTANDRHYRHVEQLCIEVFRP"
     gene            1325..2212
                     /locus_tag="PKN25_12385"
     CDS             1325..2212
                     /locus_tag="PKN25_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV91741.1"
                     /translation="MARPLRIELAGGVYHLTSRGDRREPIYADDVDRLLWLELFGEVC
                     DRFNWRCHAWCQMGNHYHLVVETAEANLSRGMRQLNGVYTQAANRRHARVGHVFQGRY
                     KAILVEADAYLVELARYVVLNPVRAGLCGDAREWPWSSHRSMLGLVDPPPWLETDWVL
                     GQFGSERTAAACRYEDFVRAGVGQPPIWERLNAQIYLGSGAFADEMAHKVPDDDRLNE
                     VPRLQRRPQGASLQAVVEGCSSRDAAIAAAAASGAFTLAQLAQFFGLHYSSISRIVAK
                     DRKARQPPGAGNASEQMSK"
     gene            2485..3597
                     /locus_tag="PKN25_12390"
     CDS             2485..3597
                     /locus_tag="PKN25_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNV91742.1"
                     /translation="MQKNLIALAVAGLAALPAFAQSNVTVYGLVDMGYKWSGDNMDDR
                     VSSRSALDSGMSAGSRLGFKGTEDLGNGMKAGFVLEQGLNLDTGTLGQGGRAFGRQSF
                     VSLGGGFGTVALGRQYPAGYLLTATVDPFGSGTAGQYNSVYLTEYRWDNAVTYVSPTL
                     GGFSISAAFTMNVNGEESPDNRGEGDVGDIRAWSVVPQYVNGPLFAGIHLQEMRAKST
                     GLYDGETVRVYDVAGTYDFGVAKLAASYGLRRASAADFSPDSAAVNGKDSRQWFVGVT
                     VPVSAAGTVRASYVARETEVVSGGPDAKVSQWALGYEHALSKRTALYAVYADIDNNEA
                     AKDSGLYASIGDAMAGGVAGGDGYQRGVNLGLRHSF"
     gene            3736..3975
                     /locus_tag="PKN25_12395"
     CDS             3736..3975
                     /locus_tag="PKN25_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNV91743.1"
                     /translation="MEATIRDLRLHTTEVLADADRGERVTITRRGQVRAVLMRCEDPG
                     GGARPSRNPACGSWRDRHEAVDEQVRGMRQPRTLP"
     gene            3975..4247
                     /locus_tag="PKN25_12400"
     CDS             3975..4247
                     /locus_tag="PKN25_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HNV91744.1"
                     /translation="MCVATDVLIWNLRDNARAADRLDGMSGFLVSAVTCMELVLGVRD
                     KGESRALRRALNFGSARVVQVDEAISARACFLVEQFALSHSMQLSK"
     gene            4244..4771
                     /gene="ampD"
                     /locus_tag="PKN25_12405"
     CDS             4244..4771
                     /gene="ampD"
                     /locus_tag="PKN25_12405"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HNV91745.1"
                     /translation="MKDLTPRCSNHDERPVGECVRLIVVHAISLPPDEFGGPGVEQLF
                     TNTLDPDAHPYYATIHHLRVSAHYFIRRDGQTLCFVDPDRRAWHAGVSCWNGRERCND
                     FSIGIELEGCDTMPFEPAQYERLAALIVALERRYPIEAVVGHSDIAPGRKTDPGPFFD
                     WKRLDALREQRRASC"
     gene            5130..6044
                     /locus_tag="PKN25_12410"
     CDS             5130..6044
                     /locus_tag="PKN25_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNV91746.1"
                     /translation="MKHATRFKALARQAGGAARSLAHGAVLSVGLMGMSWLGTQGAGA
                     ADVRYGDVLGLQAEPTASELAMASVLSFAPRRGEMGASAPSFAASIPSLAASTRLHES
                     LPVAAPGLIERKAARAVAEPIPPVFHQPALRDIAALVDNPFEDEGALSDEMERVRDWI
                     ADTYQVSEETIEPALAAAETHAEELGFDPLLIVAIMAVESSFNHRAVSNMGALGLMQV
                     IPRYHKDKIGPNRGRNVLFDPEVNVRVGTLVLHEGLQRYGSMQRALQYYNGALNDPKA
                     RYTRKVMALKKRLMLIAGRDRSSAEQAS"
     assembly_gap    6112..6211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6370..7572)
                     /locus_tag="PKN25_12415"
     CDS             complement(6370..7572)
                     /locus_tag="PKN25_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNV91747.1"
                     /translation="MTASPTFRSTLLSQRAADIQPFHVMELLRRARELEAQGRDIIHM
                     EVGEPDFGTPAPVVEAATRFLAGGDVHYTPALGLPALREAIARFYHDRFGADVAPERI
                     VVTAGASGALMLALAATTDPGDEWLLPDPGYPSNRHLIRAFEGVARALAVDAASRYQP
                     RPEQVDAAWGERTRGLMVATPSNPTGTLLSVAEIAALHRRTRARGGVLLVDEIYQGLT
                     YGVESASALADPVLNTADDVFVVNSFSKYFGMTGWRLGWLVAPAGFVRELEKLAQHFF
                     ISPSTPAQHAALAAFAPATIAILEERRHEFAQRRDVLLPALRALGFGIAAEPQGAFYV
                     YADVSALADDSEALARRMIEEAGVAATPGLDFGHHLPRRHLRIAYTTHGARLLEAAER
                     IRRLGLGS"
     gene            complement(7569..8084)
                     /locus_tag="PKN25_12420"
     CDS             complement(7569..8084)
                     /locus_tag="PKN25_12420"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNV91748.1"
                     /translation="MSTERRRAPRTSDVRRVARALQEHGVEYAVIGGIAMALHGFPRA
                     TKDIDLFLPVAAENNKRLLAALATIPESREVLRELRQEWLDQGFSTAADGEISIDLLF
                     VAAGHTFEALRPHLQTVVFEGVPIRTLDIDGMLMTKQTSRDTDLPDRKRLRQLRDALR
                     AAQAHRPPDSK"
     gene            complement(8074..8370)
                     /locus_tag="PKN25_12425"
     CDS             complement(8074..8370)
                     /locus_tag="PKN25_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91749.1"
                     /translation="MTVRHFADLESMSHHQREQELMQAIRVQGMSVAAFSAWLRQALD
                     PLQQLAGPALDFDIDAGRARARHFTSREEKQRFDEARELDRALRLALDRRRDEH"
     gene            complement(8493..>8918)
                     /locus_tag="PKN25_12430"
     CDS             complement(8493..>8918)
                     /locus_tag="PKN25_12430"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HNV91750.1"
                     /translation="MLPFCGYHMGDYFNHWLRMGHVVEDTPKIFCVNWFRMNEQGQFM
                     WPGFGDNMRVLKWIVDRCKGKISAKETALGWMPRFEDLDWNGADVGREEFEALTKVDA
                     DAWRAELNSHKEWFDKLQDRLPRQLVLKRELFELALNAD"
BASE COUNT         1410 a         2786 c         3025 g         1597 t
ORIGIN      
        1 ctcctggctt cgtatcagca agctgacaac acgagcttcg tggatgatct cgacgccgat
       61 ttgtggcaag tcggtgttgt cgttccggtt ggtgcggctg gcaacgttca cctcggctat
      121 ggcgaagttg agtatgactt cgaaggcgag tcgtcgaagg ctaagagcat tggtctggct
      181 tacacgcacg ccatgtccaa gcgcacgact gcctacgttg gttacaatta cactgacaac
      241 gacgatggcc tgaccctggg cgctgcaccg gtcggcactg ctgatatcaa catcgatgcc
      301 aacaacaagg gcacggttgc gattgaagat ggtgagaagt cgaagatgtt cgtggttggt
      361 gttcgccaca ccttctaatc tgttctcaag acaggtttga aagtaaaaac gggccttcgg
      421 gcccgttttt ttgtcgactc acgacatcga ctgtcgcgtt acacgacgct ttagaggggg
      481 gctgtggggt caggtctttc atttgctcat ttcccttccg tcggtggctt tgtgatgtaa
      541 tgttcacagg cttgtgaaac ggagttcgtc atggaagcga ccatcaggga tttgcgcctg
      601 cacacggcag aggtgcttgc ggcagcggat cgtggcgagc gggtgaccat caccagccgg
      661 gggcaggtac gggcggtgtt gatgcggtgc gaagagaacc gtgcccgggc gcatgccgag
      721 cgcaatcctg cgtttggtct gtgggcggat cggcagggca cggtagacga tgacgtgcgc
      781 aggctgcgac agccgagagt gctgccgtga tgctcgtgga tacggacgtg ctgatctgga
      841 acctgcgagg caacgcgcgt gcagcggatc ggctggatgc gatgccagga tttctggtgt
      901 cggcggtgac gtacatggag cttgtccagg gagtgcgtga caagtcggag ttgcgcgcgc
      961 ttcggcaggc cctgagcttc tggtcggcga gggtggtaca gatcgatgag gccatctccg
     1021 cgcgtgcatg cttcctttgc gagcaattcg cactttccca ttccatgcag atggcggatg
     1081 ccttgattgc agcaacggcg ctcgagcgcg ggctcgatct gctgacggca aacgatcgtc
     1141 actatcgcca tgtggagcag ctctgcatag aggtgttccg gccgtagggg agtaggggac
     1201 ccctagggtc aggtctttca tttgctcatt cgcctctgcc gtggccgagt gctcttcctt
     1261 tggtcttgcc ttttcgatta tccattcggg ataatcaggg ctgttcctgc cctgggagag
     1321 gatcatggcg cggccattgc ggatcgagct tgccggaggg gtgtaccacc tgacttcgcg
     1381 tggtgaccgt cgcgagccga tctatgccga cgacgtcgat cgcctgttgt ggctggaact
     1441 cttcggcgag gtctgcgatc ggttcaactg gcgttgtcac gcgtggtgcc agatgggcaa
     1501 ccactaccac ctggtcgtgg agaccgccga ggcgaacctc tcccggggca tgagacagct
     1561 gaacggggtg tacacccagg ccgcgaatcg acgccacgct cgtgtcgggc acgtcttcca
     1621 ggggcgctac aaggcgatcc tggtcgaggc cgacgcctac ctcgtggagc tcgcgcgcta
     1681 tgtggtgctc aaccccgtgc gggcggggct ctgtggcgat gcgcgcgagt ggccctggag
     1741 cagccatcgc tccatgttgg gtttggtcga tccgcctcct tggctggaga cggactgggt
     1801 gctggggcag ttcgggtcgg agcggaccgc ggccgcttgt cgatatgaag atttcgtccg
     1861 tgcgggcgtc ggccagccgc cgatctggga gcgcctcaac gcgcagatct acctgggcag
     1921 cggggcattc gccgacgaga tggcgcacaa ggtgccggac gatgaccgcc tgaacgaagt
     1981 gccgcggctg cagcgcaggc cgcagggcgc gtccttgcag gcggttgtgg agggctgttc
     2041 gtcgcgtgat gcggccatcg ccgcggccgc ggcctctggt gccttcaccc tcgcgcagct
     2101 cgcccagttc ttcggcctcc actacagttc gatcagccgg atcgtcgcca aggaccggaa
     2161 ggcgcgccag cctcccggtg ccgggaatgc aagcgagcaa atgagtaaat gaaagacctg
     2221 accccgctgt cccccacacg tctcgctcgg gcgggcgcaa gctggccggg atcgccgaac
     2281 ggctgcggcc cgcgcccgcg cctgcgctcc gcaaccgcaa cgcctggttc gcaggaccgg
     2341 gttcgagtgc taatcgggtt aacaaatcag cctgctgcat taaccattcc gcgcctgcca
     2401 ttaacaccct ctgcctatcc ttgcctgcgg gttccggttg ctgcatgaga ccggattaca
     2461 gcgtttcgat ccaaggagaa gcagatgcag aagaacttga tcgcccttgc cgttgccggg
     2521 ctcgccgccc tgccggcctt cgcgcagtcc aatgtgacgg tctacggcct cgtcgacatg
     2581 ggctacaagt ggtcgggcga caatatggac gatcgcgtgt ccagccggtc ggcgctcgat
     2641 tcgggcatga gtgcaggaag ccgcctcggc ttcaagggca ccgaggatct gggtaacggc
     2701 atgaaggcgg gcttcgtgct cgagcagggc ctcaatctcg ataccgggac gctcggccag
     2761 ggcggtcgcg ctttcgggcg ccagtccttc gtcagcctcg gcggcggctt cgggaccgtc
     2821 gcgctcgggc gccagtaccc ggccggctac ctgctgacgg cgacggtcga ccccttcggc
     2881 agcggcaccg cggggcagta caacagcgtc tacctgaccg aataccgctg ggataacgcc
     2941 gtgacttacg tctccccgac cttgggcggg ttctcgatct ccgcggcctt cacgatgaac
     3001 gtcaatggcg aggagtcgcc ggacaaccgt ggagaggggg acgtcgggga catccgtgcg
     3061 tggtccgtcg tgccgcaata cgtgaacggc cccctgtttg cgggcatcca cctccaggag
     3121 atgcgtgcca agtccaccgg cctgtacgac ggcgagacgg tgcgcgtgta tgacgtcgcc
     3181 ggcacgtacg atttcggtgt cgccaagctc gccgcctcgt acgggctgcg tcgcgccagc
     3241 gctgccgatt tcagccccga ttcggcggcc gtgaacggaa aggactcccg ccagtggttc
     3301 gtcggcgtga ccgtgccggt cagcgcggcc ggcaccgtgc gcgcctccta cgtggcgcgc
     3361 gagaccgaag tcgtttccgg cgggcccgac gcgaaagtca gccagtgggc gctcggctac
     3421 gagcatgcac tgtcgaaacg cactgccctc tacgcggtgt atgcggacat cgacaacaac
     3481 gaagccgcga aggacagcgg cctgtatgcc tcgatcggcg atgcgatggc tggcggcgtc
     3541 gcgggcgggg acggctacca gaggggcgtg aacctgggcc tgcggcatag cttctgagtg
     3601 ggcttcgtgc ggggccctct gggtcaggtc ggtcatttgc tcatctgttc ttcgtcggag
     3661 acggtttcgg ggtcagtgat acccacaggc gctcccgtga ggtactgttc acgcgtttgc
     3721 gaaacggagt tcgtcatgga agcgacgatt cgggatttgc gcctgcacac gacggaggtg
     3781 ctcgcggatg ccgaccgcgg tgagcgcgtg acgatcaccc gtcgtggcca ggtgcgggcg
     3841 gtgttgatgc gttgcgaaga ccctggagga ggcgcgcgac cgtcgcgcaa tccggcatgc
     3901 ggctcatggc gcgatcggca cgaggcggtg gatgagcagg tacggggaat gcggcagcca
     3961 aggacattgc cgtgatgtgc gtcgccaccg atgtgctgat ctggaacctg cgcgacaatg
     4021 cgcgggccgc cgatcgcttg gatgggatga gcggtttcct cgtttctgcc gttacctgca
     4081 tggaactcgt gctgggggtt cgcgacaaag gcgagtcgcg tgcacttcgg cgggccttga
     4141 acttcgggtc ggcgcgggtc gtgcaggtag acgaggccat ttctgcgcgt gcctgctttc
     4201 ttgtagagca gttcgccctg tcgcattcca tgcagttgag caaatgaaag acctgacccc
     4261 acggtgttcc aaccacgacg agcgcccagt gggcgagtgc gtccgcctca tcgtcgtcca
     4321 cgccatcagc ctccctcccg acgagttcgg cggtcccggc gtcgagcaac tcttcaccaa
     4381 cacgctggat cccgacgccc acccctacta cgcgaccatc caccacctgc gcgtgtcggc
     4441 gcactacttc atccgtcgcg acggacagac gctgtgcttc gtcgaccccg accgccgcgc
     4501 ctggcatgcc ggggtgtcgt gctggaacgg gcgcgagcgc tgcaacgact tctccatcgg
     4561 catcgagctc gagggttgcg acacgatgcc tttcgagccc gcgcagtacg agcgtctggc
     4621 ggcgctgatc gtcgcgctgg agcggcgcta tccgatcgag gcggtggtgg gacactccga
     4681 catcgcgccc ggtcgcaaga ccgatccggg gccttttttc gactggaagc gccttgatgc
     4741 gttgcgcgaa cagcgccgcg caagctgctg atcggttgaa acttcccgca aattcaagtg
     4801 tttcttgctg ttgccctggg ggcgtcaggt tttgacaata aacctgtgag cgaatacaat
     4861 ccgctccctg ttgcattgca acattcctaa tccgatctcg gtgcggcgcc tcctggaagg
     4921 gagacgcagt ttcatggacg cacgcaccgc ggggcccgtc gtctggttgc ggcgatgggt
     4981 cttaacacct actggcgacc gagtactcct gcccgtcgtg ccggccaacc cgccgagcga
     5041 cgcggatgct gcccgacagg gcggcaccgg gcggccccca ccacagggct cttccacggc
     5101 aggactcagg ccacagcatg gaggacacca tgaaacacgc aactcgattc aaggcgctcg
     5161 cgcgtcaggc gggcggcgct gcgcgcagtc tggcgcacgg tgcagttctt tccgttggtc
     5221 tcatgggcat gagctggctc ggcacgcaag gggcgggtgc ggcggatgtg cgctacggcg
     5281 acgtcctcgg cctgcaggcc gaaccgaccg cttccgaact ggccatggcc tcggtgctgt
     5341 cctttgcccc ccgccggggc gagatgggcg cttcggctcc ctccttcgcg gcctcgattc
     5401 cgtccctcgc cgcctccacc cgcttgcacg agagcttgcc ggtggccgcg ccggggctga
     5461 tcgagcgcaa ggccgcgcgc gcagtggcgg agccgatccc gccggtcttc caccagcctg
     5521 cgctgcgcga catcgcggcg ctggtcgaca acccattcga ggacgagggc gcgctctcgg
     5581 acgagatgga gcgtgtgcgc gactggatcg ccgacaccta ccaggtttcc gaggagacga
     5641 tcgagcccgc gctggccgcg gccgagacgc acgccgagga actcggtttc gaccccctgc
     5701 tgatcgtcgc gatcatggcg gtggagtcga gcttcaacca tcgcgcggtc agcaacatgg
     5761 gcgcgctcgg gttgatgcag gtgattccgc gctatcacaa ggacaagatc ggtcccaatc
     5821 gcggccgcaa cgtcctgttc gatcccgagg tcaatgtccg cgtcggcacc ctggtgctgc
     5881 acgaggggct gcagcgctac ggcagcatgc agcgcgcgct gcagtactac aacggtgcgc
     5941 tcaacgaccc gaaggcacgc tacacgcgca aggtcatggc gctgaagaag cgcctgatgc
     6001 tgatcgccgg ccgcgatcgt tccagcgcgg agcaggcgag ctgagttcct gcgccctgca
     6061 agcgcgcggg cggggcgtcg gacgggggat cgcgacttgc gtcgctccta cnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncggggcggt ttcgagggcg tggggcgttg
     6241 ctgcatccta cgccggggct ctgcgggtct tgccgtagga gcgacgcaag tcgcgattcg
     6301 ggcgccggtc gaagtttggc gccggtcgca ggcgccgacc gcccacccct cgggcgagcg
     6361 gcctccggct cagcttccca gtcccagccg gcggatccgc tccgcagcct ccagcagtcg
     6421 cgcgccgtgc gtggtgtacg cgatgcgcaa gtggcggcgg ggtaggtggt ggccgaagtc
     6481 caggccgggc gtcgcggcca cgcccgcttc ctcgatcatc cggcgcgcga gcgcttccga
     6541 gtcgtcggcg agtgccgaca cgtccgcata cacgtagaac gccccctgcg gctccgcggc
     6601 gatgccgaag ccgagcgcgc gcagcgccgg cagcagcacg tcgcggcgct gggcaaactc
     6661 gtggcggcgc tcctccagga tcgcgatcgt cgccggcgcg aaggccgcga gcgcggcgtg
     6721 ctgcgccggc gtcgacggcg agatgaagaa gtgctgggcg agcttctcga gctcgcgcac
     6781 gaagcccgcc ggcgccacca gccagccgag ccgccagccg gtcatgccga aatacttcga
     6841 gaagctgttc accacgaaga cgtcgtcggc cgtgttcagc accgggtcgg caagtgcgct
     6901 ggccgattcc acgccgtagg tcagcccctg gtagatctcg tcgaccagca gcacgccgcc
     6961 acgcgcgcgg gtgcgccggt gcagcgcggc gatctccgcg accgacagca gcgtgccggt
     7021 ggggttggac ggtgtcgcca ccatcagccc gcgggtgcgc tcgccccagg cggcgtcgac
     7081 ctgctcgggg cggggctggt agcggctcgc ggcatcgacc gcgagcgcgc gcgcgacccc
     7141 ttcgaaggcg cggatcaggt gacggttgga gggatagccg gggtcgggca gcagccactc
     7201 gtcgcccgga tcggtggtgg cggcgagcgc cagcatcagc gcgcccgagg cgccggcggt
     7261 gacgacgatg cgctcgggcg cgacgtcggc gccgaagcgg tcgtgataga agcgcgcgat
     7321 cgcctcacgc agcgccggca ggccgagcgc cggggtgtaa tgcacgtcgc cgccggcgag
     7381 gaagcgagtg gcggcctcga ccaccggcgc cggggtgccg aagtcgggct cgccgacctc
     7441 catgtggatg atgtcgcgcc cctgcgcctc gagttcgcgc gcgcggcgca gcagttccat
     7501 gacgtggaag ggctggatgt cggcagcgcg ttgcgagagc agcgtagagc ggaaagtcgg
     7561 ggaagcggtc atttcgagtc cggggggcgg tgtgcctgcg ctgcacgcag ggcgtcgcgc
     7621 agttgtcgca agcgtttgcg gtcgggtaga tcggtatcgc gcgaggtctg tttcgtcatc
     7681 agcatgccgt cgatatcgag ggtgcggatc gggacgcctt cgaagaccac cgtctggagg
     7741 tgtgggcgaa gcgcctcgaa ggtgtggccc gccgcgacga acaggagatc gatgctgatt
     7801 tcgccgtccg cagcggtcga aaagccctga tccagccact cctgcctgag ctctcgcaag
     7861 acctcgcggc tttccgggat cgtcgccagt gcggccagca agcgcttgtt gttctcggcc
     7921 gcgacgggca ggaaaaggtc gatgtccttc gtcgcacgcg gaaagccgtg cagggccatc
     7981 gcgattccgc cgatcaccgc atactcgacg ccgtgttcct gcagggctcg tgcgactcgc
     8041 cggacgtcgc tggtccgtgg agcccgtctc ctttcagtgc tcatcacgac gccggtcgag
     8101 cgcaagtcga agcgcgcgat cgagttcgcg cgcctcgtcg aagcgctgct tttcttcgcg
     8161 gctggtgaag tggcgcgccc tcgcccggcc ggcgtcgata tcgaaatcga gcgcggggcc
     8221 ggcaagctgc tggagcgggt cgagggcctg cctgagccag gcgctgaagg cagcgaccga
     8281 catgccctga acacggattg cctgcatcag ctcctgctcg cgctggtgat gcgacatcga
     8341 ctccaggtcg gcaaagtgtc gaaccgtcat gatcgttttc cctggcctcg ggagcagcta
     8401 gtctagcctg tccccccagc cgcttcaatt tcgggcttga aaaagaaaaa gcgccggact
     8461 gcggcgcttt ttcggagcgg gcaagctcag gctcagtccg cgttcagcgc cagttcgaac
     8521 agctcgcgct tgagcaccag ctggcgcggc aggcggtcct gcagcttgtc gaaccactcc
     8581 ttgtgcgagt tcagctcggc acgccaggcg tcggcgtcga ccttggtgag cgcctcgaac
     8641 tcctcgcggc cgacgtcggc gccgttccag tcgaggtcct cgaagcgcgg catccagccc
     8701 agcgcggttt ccttcgcgga gatcttgccc ttgcagcggt cgacgatcca cttgagcacg
     8761 cgcatgttgt cgccgaagcc cggccacatg aactggccct gctcgttcat gcggaaccag
     8821 ttcacgcaga agatcttggg ggtgtcctcg accacgtggc ccatgcgcag ccagtggttg
     8881 aagtagtcgc ccatgtggta gccgcagaag ggcagcat
//
LOCUS       DAOQBS010000126         8830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_7379_length_8830_cov_14.640912, whole genome shotgun sequence.
ACCESSION   DAOQBS010000126 DAOQBS010000000
VERSION     DAOQBS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7379_length_8830_cov_14.640912"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1695
                     /locus_tag="PKN25_12435"
     CDS             <1..1695
                     /locus_tag="PKN25_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNV91751.1"
                     /translation="SGVLMPLLSRLKINPVLGFLSVGVLLGPYGLASLAKAWPPLSWL
                     TFARPDQVEFLAELGVIFLMFMIGLEMSVERLWSMRRLVFGLGGLQVALSAAAVGGLA
                     LWFGNGIEAAVILGVVLAFSSTAIVMQLLIQRHELGTPLGQASFAILLFQDLAVVPLL
                     VLISILGAASGEGSFASLLGLAMLKGVLTVVGIYLVGRRVVRPLFHQIAVDKQPDTFT
                     ALTLLTTLGVAALTWVAGLSMALGALLAGLIIAETEFRHEVEITIEPFKGLLMGLFFM
                     SVGMGIDLRALLAEPLWIPLSVVGLLAMKALIVFVLLRVFGLSWGRSAEGGLLLGQGG
                     EFAFIVIGMALTLGLLERDVGQFMLIVVGVSMLAAPVIARLGQSLGDRIDRRIAPPAE
                     ADDAELGELGGHVIIAGYGRVGQLVGQMLEEQGVGYVAIENNAQLIAHQRKAGVPLVF
                     GDASRPELLRKLRLDQARAVVLTMDHTAAAIHAVHGIRRMVPMMRIIARARDEKHALL
                     LREAGASVVVPETLESSLKLTGWVLETLGIPPDAALRLVELERERRIVALRDTGNA"
     gene            1721..2743
                     /locus_tag="PKN25_12440"
     CDS             1721..2743
                     /locus_tag="PKN25_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNV91752.1"
                     /translation="MIPQHNALQILRKPRIALLATGGTIAGTAGSATDTRDYTAGQLG
                     PAALLAAVPQLAELARIDATQIFSVDSKDMTPQHWLVLSRRVQRLMDDPAVDGIVITH
                     GTDTLEETACFLNVTVATTKPVVMTAAMRPATALSADGPLNLYQAVELACRPAAARSG
                     VLAVVGDRILPAAALAKRHPSAVDAFSFEGALTTLASDAALHALAPSRYSRVVLPDEL
                     QHLPPVELLTVAAGSSPALASAVARALARAQLLNGAAGLVLALPGNASLPEAWRPALE
                     ELPNAVPVVLASRCGGGVRRHHGNAPANWLDAGALDPLKARVRLIAGLGAGLRGARLR
                     RWFERA"
     gene            complement(2777..3718)
                     /gene="cysB"
                     /locus_tag="PKN25_12445"
     CDS             complement(2777..3718)
                     /gene="cysB"
                     /locus_tag="PKN25_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HNV91753.1"
                     /translation="MNLQQLRYVHEVARRGLNVSEAADALFTSQPGVSKQIRQLESEL
                     GIEIFTRHGKRLVAVTEPGRAVLAIAERVLRDLDNLQQVGDEFANEAVGRLSIATTHT
                     QARYVLPDVIRRFMQRYPQVKLELHQGNPRQVCEMVLDGSADIAIATEAIAEYDELVM
                     LPCYQWNRCVVATPRHPILQAAPLTLEAIARYPLITYDDAFTGRGQINKAFLGRGLKP
                     KVVLTAIDSDVIKKYVEMDLGIGILARMAYDPAEDRRLGMVDAAHLFESSTTRIGLRR
                     RAWLRAYVYAFIEGFAPHLSRRMVETALAGGGTDPGL"
     gene            3823..4605
                     /locus_tag="PKN25_12450"
     CDS             3823..4605
                     /locus_tag="PKN25_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNV91754.1"
                     /translation="MDFAYTIAGFAVGAIVGLTGVGGGSLMTPLLVLMFGIHPSVAVG
                     TDLLYAAITKAGGTLAHGLKGTVDWKITRLLATGSIPAAAITLALIGHFAPGGIEGAA
                     HLIQVSLGVALVLTAVALIFRKRIQAYAATRSGGRPADPARTARLTVLTGAVLGVLVS
                     ISSVGAGALGVTALFFLYPAMPALRIVGSDIAHAVPLTAVAGIGHWFLGSVDWLLLGS
                     LLVGSLPGIWLGSHISTKVPDRVLRPILATMLVLVGAKLMAH"
     gene            4674..6356
                     /locus_tag="PKN25_12455"
     CDS             4674..6356
                     /locus_tag="PKN25_12455"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNV91755.1"
                     /translation="MYQYDEHDQRLLDQRVAQFRDQMRRHLAGELSADEFRPLRLQNG
                     LYIQRHAPMFRISVPYGHLASRQLRKLAHLARSYDRGYAHFTTRTNVQFNWPKLEDVP
                     EMLAELASVQMHANQTSGNCIRNITADPFAGVAADEVADPRPFAEILRQWATYNPEFA
                     FLPRKFKIAFNSAAEDRVVLRVYDIGLDLVRNEAGELGFRVLVGGGLGRTPILGEEIK
                     PFLPWQHLISYCEAILRVYNRWGRRDNLWKARIKILVKALGPEEFGRQVEEEWANGKD
                     GPNTITAAELARVSAHFAPPAYETLPAQDAAFDELLAGNKAFATWVKRNVRAHRQPGY
                     AAVVLSLKKTGTAPGDVTAEQMDLIADLADRFSFGEARMLHEQNVALADVRRTDLFEL
                     WQAARAAGLATPNIGLLTDIICCPGGDFCSLANARSIPIADAIQRRFDDLDYQHDIGE
                     IDLNISGCMNSCGHHHVGHIGILGVDKNDEEWYQVSIGGTQGNGTTLGKVIGPSFALD
                     EIVGVIEKLLETYVELRHEDERFIDTVQRVGTEPFKARVYADRQPQRKAAHV"
     gene            6349..6903
                     /locus_tag="PKN25_12460"
     CDS             6349..6903
                     /locus_tag="PKN25_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HNV91756.1"
                     /translation="MSDKIIKNGRVVADDRLTVSLAEGEVPADIAVPAGPVLVPLAVW
                     QARREELLARARSGEVGVWLAAGEDVAALADDLGLLQIVGLHFPKFTDGRSYSAATLL
                     RTRYGYQGELRAIGQVLRDQFNYMTRCGFDALQPQAERYTDEQLEAAVASIDDFTQPY
                     QRSVKDPLPLFRRVERGTGREVQQ"
     gene            6900..7670
                     /locus_tag="PKN25_12465"
     CDS             6900..7670
                     /locus_tag="PKN25_12465"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNV91757.1"
                     /translation="MNAAVSLLRPAPTNAATRPQLTDALRATVGAKSAATLALLREAV
                     AEFGAHGELAFANSFGAEDMVLTDLILSNGLPITIFSLDTGRLPAETYSLIGEVERRY
                     GTKLEIYFPDAGAVETYVRSHGINAFYDTVELRKACCGVRKMEPLRRALTGRKAWITG
                     LRAAQSVTRSGLPVRETDTANGLEKLNPLSDWSETEVWAYIRLAEVPYNALHDQFYPS
                     IGCAPCTRAIAVGEDVRAGRWWWENPETKECGLHVKAS"
     gene            7674..7826
                     /locus_tag="PKN25_12470"
     CDS             7674..7826
                     /locus_tag="PKN25_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91758.1"
                     /translation="MNGKHPAQGTHSPAIGAGRRSLQQRLLALRAGLRAALRAFRTHP
                     IKHPQR"
     gene            7834..8748
                     /gene="cysD"
                     /locus_tag="PKN25_12475"
     CDS             7834..8748
                     /gene="cysD"
                     /locus_tag="PKN25_12475"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNV91759.1"
                     /translation="MSTLTKQTLSHLDWLEAEAIHILREVAGQCANPVLLFSGGKDSI
                     CLLRLAEKAFRPGRFPFPLMHIDTGHNYKEVTDFRDRRAAELGERLIVRSVEDSMARG
                     TVVLKHAGESRNKHQSVTLLEAIEEFGFDACIGGARRDEEKARAKERIMSFRDEFGQW
                     DPKNQRPELWNLYNARSHKGENIRAFPISNWTELDVWQYIQREQLELPSIYFAHTRPV
                     VRKNGLLQPVTELTPAKDGDVVEDVRVRFRTVGDITCTAPVESDADTVEKILAETATT
                     TITERGATRMDDQTSEASMEQRKKEGYF"
BASE COUNT         1369 a         3268 c         2872 g         1321 t
ORIGIN      
        1 agcggtgtgc tgatgccgct gctgtcgcgc ctgaagatca accccgtgct cggcttcctg
       61 tcggtcgggg tgctgctcgg cccctacggc ctcgcctcgc tcgcaaaggc ctggccgccg
      121 ctgtcgtggc tcaccttcgc gcgccccgac caggtggagt tcctcgccga gctcggagtc
      181 atcttcctga tgttcatgat cggcctggag atgtcggtcg agcgcctgtg gtcgatgcgc
      241 cggctggtat tcgggctcgg cggcctgcag gtcgcgctct cggcagcggc ggtcgggggg
      301 ctcgcgctgt ggttcggcaa cggcatcgag gccgcggtca tactcggcgt ggtgctggcc
      361 ttctcgtcca ccgcgatcgt catgcagttg ctgatccagc gccacgaact cggcacgccg
      421 ctcggccagg cctcgttcgc gatcctgctg ttccaggacc tcgcggtggt gccgctgctg
      481 gtgctgatca gcatcctcgg tgccgcctcg ggcgagggca gcttcgcgag cctgctcggc
      541 ctcgcgatgc tcaagggcgt gctcaccgtg gtgggcatct acctcgtcgg tcgccgcgtg
      601 gtccgtccgc tcttccacca gatcgcagtc gacaagcaac ccgacacctt caccgccctc
      661 accctgctga ccaccttggg cgtggcggcg ctgacctggg tggccggcct gtcgatggcg
      721 ctcggcgccc tgctcgccgg cctgatcatt gccgagaccg agttccgcca cgaggtggag
      781 atcaccatcg agcccttcaa gggcctgctg atgggcctgt tcttcatgtc ggtgggcatg
      841 ggcatcgacc tgcgcgcgct gctcgccgag cccttgtgga ttccgctctc ggtggtcggt
      901 ctgctggcca tgaaggcgct catcgtgttc gtgctgctgc gcgttttcgg cctgtcctgg
      961 gggcgctccg ccgagggcgg cctgctgctc ggccaaggcg gcgagttcgc cttcatcgtg
     1021 atcggcatgg cgctcacgct gggcctgctc gaacgcgatg tgggccagtt catgctgatc
     1081 gtggtcggcg tgtcgatgct cgccgcgccg gtgatcgcgc gcctcgggca gagcctgggc
     1141 gaccgcatcg accgccgcat cgcgccgcct gccgaggcgg acgacgccga gctcggcgag
     1201 ctcggcgggc atgtgatcat cgccggctac ggccgcgtcg gccagctggt cgggcagatg
     1261 ctggaagagc agggcgtggg ctacgtcgcc atcgagaaca atgcccagct catcgcccac
     1321 cagcgcaagg cgggggtgcc gctggtgttc ggcgacgcca gccgcccgga gctgctgcgc
     1381 aagctgcgcc tcgaccaggc gcgcgcggtg gtgctgacca tggaccacac cgccgccgcg
     1441 atccacgcgg tgcacggcat ccgccgcatg gtgccgatga tgcggatcat cgcccgcgcg
     1501 cgcgacgaga agcatgccct gctgctgcgc gaggccggcg cctcggtggt ggtgcccgaa
     1561 accctcgagt ccagcctcaa gctcaccggc tgggtgctgg agaccctcgg catacccccc
     1621 gatgccgcgc tacgcctggt cgagctggaa cgcgagcggc gcatcgtcgc gctgcgcgac
     1681 accggcaacg cctgaccgcc ccctgacgaa tcccgcccac atgatcccgc agcacaacgc
     1741 cctccagatc ctgcgcaagc cgcgcatcgc gctcctcgcc accggcggca ccatcgccgg
     1801 cacggccggc tcggccaccg acacccgcga ttacaccgcc ggccagctcg gccccgcggc
     1861 gctgctcgcc gccgtgcccc agttggccga gctcgcccgc atcgacgcca cgcagatctt
     1921 cagcgtcgac agcaaggaca tgacgccaca gcactggctg gtgctgtcgc gccgggtgca
     1981 gaggctgatg gacgaccccg cggtcgacgg catcgtcatc acccacggca ccgacacgct
     2041 ggaagagacg gcctgtttcc tcaacgtcac cgtcgccacc accaagccgg tggtgatgac
     2101 tgcggcgatg cgcccggcca ccgcgctatc ggccgacggc ccgctcaacc tctaccaggc
     2161 ggtcgagctc gcctgccgcc cggcggccgc acgctcgggc gtgctcgcgg tggtcggcga
     2221 ccgcatcctg ccagccgcgg ccctcgccaa gcgccacccc tcggcggtcg atgcgttctc
     2281 gttcgagggc gcgctcacca cgctcgccag cgacgccgcg ctgcacgcgc tcgcgccctc
     2341 gcgctacagc cgtgtcgtgc tgccggacga gttgcagcac ctgccgccgg tggagctgct
     2401 caccgtcgct gcgggcagct cgcccgcact cgcctcggcg gtggcgcgcg cgctcgcacg
     2461 cgcccagctg ctcaatggcg ccgcaggcct ggtgctggcc ctgcccggca acgccagcct
     2521 gcccgaggcc tggcgacccg cgctggaaga actgcccaac gcggtaccgg tggtgctcgc
     2581 cagccgctgc ggcggcggcg tgcgtcggca ccatggcaac gcccccgcca actggctcga
     2641 cgccggcgcg ctcgacccgc tcaaggcgcg cgtgcgcctg atcgcgggcc tcggcgccgg
     2701 attgcgcggc gcacgcctgc ggcgctggtt cgagcgcgcc tgacgcggcg gtgcttgcgt
     2761 cgccccggcg cgcccctcac agccccggat cggtgccgcc gccggcgagc gcggtctcga
     2821 ccatgcgccg cgacagatgc ggcgcgaagc cctcgatgaa ggcgtacaca taggcgcgca
     2881 gccaggcgcg acggcgcagg ccgatccgtg tcgtgctcga ctcgaacagg tgggccgcat
     2941 cgaccatgcc gagcctgcga tcctccgcgg ggtcgtaggc catgcgcgcg aggatgccga
     3001 tgcccaggtc catctcgaca tacttcttga tcacgtccga gtcgatcgcg gtgagcacga
     3061 ccttgggctt gaggccgcga ccgaggaagg ccttgttgat ctggccgcgg ccggtgaagg
     3121 cgtcgtcgta ggtaatcagc ggatagcgcg cgatcgcctc gagcgtgagc ggcgcggcct
     3181 gcaggatggg gtggcgcggg gtggcgacca cgcagcggtt ccactggtaa cagggcagca
     3241 tcaccagctc gtcgtactcg gcgatggcct cggtggcgat cgcgatgtcg gcgctgccgt
     3301 cgagcaccat ctcgcaaacc tggcgcggat tgccctggtg cagctcgagc ttgacctgcg
     3361 ggtagcgctg catgaagcgg cggatcacgt cgggcagcac gtagcgcgcc tgggtatggg
     3421 tggtcgcaat cgacaggcgg cccacggcct cgttggcgaa ttcgtcaccc acctgctgca
     3481 ggttgtcgag gtcgcgcagc acgcgctcgg cgatcgccag caccgcccgc cccggctcgg
     3541 tgaccgccac caggcgcttg ccgtggcgag tgaagatctc gatgccgagc tcggactcga
     3601 gctggcggat ctgcttggac acccccggct gcgaggtgaa cagcgcgtcg gccgcctcgg
     3661 agacattgag cccgcgacgg gcgacttcat gcacgtaacg cagttgctgc aggttcatgg
     3721 cgcgctagat ataacaacca gttcttaaag tctaactgca aaccgcttct tgcgcttaac
     3781 tgaagtcctt acgatgcgcc gcaacatcca ggagcgcgtt acatggactt cgcctacacc
     3841 atcgccgggt tcgcagtcgg tgccatcgtc ggtctgaccg gcgttggagg cggatccttg
     3901 atgacgccgc tgctggtgct gatgttcggc atccacccct cggtcgccgt cggcaccgac
     3961 ctgctctacg ccgcgatcac caaggccggc ggcaccctgg cgcacggact caagggcacg
     4021 gtggactgga agatcacccg cctgctcgcc accggcagca ttcccgcggc ggcgatcacg
     4081 ctggcgctga tcggacactt cgcgcccggc ggcatcgaag gcgcggccca cctgatccag
     4141 gtctcgctcg gcgtcgcctt ggtgctgacc gcggtggcgc tgatcttccg caagcgcatc
     4201 caggcctatg ccgcgacgcg ctcgggtggc cgccccgccg atccggcgcg caccgcccgc
     4261 ctgaccgtgc tcaccggcgc cgtgctcggc gtgctggtgt cgatctcctc ggtgggcgcc
     4321 ggcgcgctcg gcgtcaccgc gctgttcttc ctctacccgg cgatgccggc gctacgcatc
     4381 gtcggctcgg acatcgccca cgccgtgccg ctgaccgcgg tcgccggcat cggccactgg
     4441 tttctcggca gcgtcgactg gctgctgctg ggcagcctgc tggtcggctc gctgcccggc
     4501 atctggctcg gcagccacat ttccaccaag gtacccgacc gcgtcctgcg cccgatcctg
     4561 gcgacgatgc tggtgctggt cggcgccaag ctgatggcgc actgagccgc agcgaacaag
     4621 cacgcgacgc ccctccccgg cccgaatcca cgcatacgac agagagagcc catatgtacc
     4681 aatacgacga acacgaccag cgcctgctcg accagcgcgt cgcccagttc cgcgaccaga
     4741 tgcggcgtca cctggccggc gagctctcgg ccgacgagtt ccgtccgctg cgcctgcaga
     4801 acgggttgta catccagcgc cacgcgccga tgttccgcat ctcggtgccc tacggccacc
     4861 tcgccagccg ccagctgagg aagctcgccc acctcgcgcg cagctacgac cgcggctacg
     4921 cccacttcac cacccgcacc aacgtgcagt tcaactggcc gaagctggaa gacgtgccgg
     4981 agatgctcgc cgaactggcc agcgtgcaga tgcacgccaa ccagacctcg ggcaactgca
     5041 tccgcaacat caccgccgac cccttcgccg gcgtggccgc cgacgaggtc gccgatccgc
     5101 gccccttcgc cgagatcctg cgccagtggg cgacctacaa cccggaattc gccttcctgc
     5161 cgcgcaagtt caagatcgcc ttcaacagcg ccgccgaaga ccgcgtggtg ctgcgggtgt
     5221 acgacatcgg cctcgacctg gtccgtaacg aggccggcga gctcggcttc cgcgtgctgg
     5281 tgggcggcgg cctcggccgc accccgatcc tgggcgagga gatcaagccc ttcctgccct
     5341 ggcagcacct gatcagctac tgcgaggcca tcctgcgcgt ctacaaccgc tggggccgcc
     5401 gcgacaacct gtggaaggcg cgcatcaaga tcctcgtcaa ggcgctcggg ccggaagagt
     5461 tcggccgcca ggtggaggaa gaatgggcca acggcaagga cggccccaac accatcaccg
     5521 cggcggagct ggcgcgcgtg tcggcccact tcgccccgcc tgcctacgag accctgcccg
     5581 cgcaggacgc cgccttcgac gaactcctcg ccggcaacaa ggccttcgcc acctgggtca
     5641 agcgcaatgt gcgcgcccac cgccagcccg gctacgcggc ggtggtcctg tcgctgaaga
     5701 agaccggcac cgccccgggc gacgtcaccg ccgagcagat ggacctgatc gccgatctcg
     5761 ccgaccgctt cagcttcggc gaggcgcgca tgctgcacga gcagaacgtc gcgctcgccg
     5821 acgtgcgccg caccgacctc ttcgaactgt ggcaggcggc gcgtgccgcc ggcctggcga
     5881 cgccgaacat cggcctgctg accgacatca tctgctgccc cggcggcgac ttctgcagcc
     5941 tcgccaacgc acgctcgatc ccgatcgccg acgccatcca gcgccgcttc gacgacctcg
     6001 actaccagca cgacatcggc gagatcgacc tcaacatctc cggctgcatg aactcctgcg
     6061 gccaccacca cgtcggccac atcggcatcc tcggcgtcga taagaacgac gaggagtggt
     6121 accaggtcag catcggcggc acacagggca acggcaccac gctcggcaag gtgatcggtc
     6181 cgtccttcgc cctcgacgag atcgtgggcg tgatcgagaa gctgctcgaa acctacgtcg
     6241 aactgcgcca tgaggacgag cgcttcatcg acaccgtaca acgcgtcggc accgagccct
     6301 tcaaggcacg cgtgtatgcc gaccgccagc cgcaaaggaa agccgcccat gtctgacaag
     6361 atcatcaaga acggccgcgt cgtcgccgac gaccgcctga ccgtgagcct cgccgaaggc
     6421 gaggtgcctg ccgacatcgc ggtgcccgcc ggccccgtgc tggtgccgct cgccgtatgg
     6481 caggcgcgcc gcgaagagct gctggcgcgt gcgcgcagcg gcgaggtggg cgtgtggctg
     6541 gccgcgggcg aggacgtcgc cgcactcgct gacgacctcg ggctgctgca gatcgtcggc
     6601 ctgcacttcc ccaagttcac cgacggccgc agctactccg cggcaacctt gctgcgcacc
     6661 cgctacggct accagggcga actgcgcgcg atcggccagg tcctgcgcga ccagttcaac
     6721 tacatgacgc gctgcggctt cgatgccctg cagccgcaag ccgagcgcta caccgacgaa
     6781 cagctcgagg cggcggtggc cagcatcgac gacttcaccc agccctacca gcgctcggtg
     6841 aaggacccgc tgccgctgtt ccgccgcgtc gagcgcggca ccggccgcga ggtgcagcaa
     6901 tgaacgccgc cgtcagcctg ctgcgccccg ccccgaccaa cgccgccacc cgcccgcagc
     6961 tcaccgacgc actgcgcgcc accgtgggtg ccaagtccgc agccacgctc gcactgctgc
     7021 gcgaggccgt cgccgagttc ggcgcgcacg gcgagctcgc cttcgccaac agcttcggcg
     7081 ccgaggacat ggtgctgacc gacctcatcc tgtcgaacgg cctgccgatc accatcttct
     7141 cgctcgacac cgggcggctc cccgccgaga cctacagcct gatcggcgaa gtcgagcgcc
     7201 gctacggcac gaagctcgag atctacttcc ccgacgcggg cgcggtcgaa acctatgtgc
     7261 gcagccacgg catcaacgcc ttctacgaca cggtcgagct gcgcaaggcc tgctgcggcg
     7321 tgcgcaagat ggagccgctg cgtcgcgcgc tcaccggccg caaggcctgg atcaccggcc
     7381 tgcgcgcagc ccagtcggtc acccgctccg gcctgccggt acgcgagacc gacaccgcca
     7441 acgggctgga gaagctcaac ccgctgtcgg actggagcga gaccgaggtg tgggcctaca
     7501 tccgcctcgc cgaggtgccc tacaacgccc tgcacgacca gttctacccc agcatcggct
     7561 gcgccccctg cacccgcgcg atcgcggtcg gcgaggacgt gcgcgccgga cgctggtggt
     7621 gggaaaaccc ggagaccaag gagtgcggtc tccacgtcaa ggcgagctga gccatgaacg
     7681 gcaagcaccc cgcccagggc acgcacagcc ccgcgatcgg tgccggccgc cgcagcctgc
     7741 agcagcgcct gctcgcgctg cgcgccggac tcagggcagc gctgcgcgcc ttcagaaccc
     7801 atccgatcaa gcatccgcaa cggtaaccgc accatgtcca cgctgaccaa acagacgctg
     7861 tcccacctcg actggctcga ggccgaagcc atccacatcc tgcgcgaagt cgccggccag
     7921 tgcgccaacc cggtgttgct gttctccggc ggcaaggatt cgatctgcct gctgcgcctg
     7981 gccgagaagg cctttcgccc gggccgcttc cccttcccgc tgatgcacat cgacaccggc
     8041 cacaactaca aggaagtcac cgacttccgc gaccggcgcg ccgccgaact gggcgagcgc
     8101 ctgatcgtgc gctcggtgga agactcgatg gcgcgcggca ccgtggtgct gaagcacgcg
     8161 ggcgagtcgc gcaacaagca ccagtcggtg acgctgctcg aggcgatcga ggagttcggc
     8221 ttcgacgcct gcatcggcgg cgcccgccgc gacgaggaga aggcgcgtgc caaggaacgc
     8281 atcatgagct tccgcgacga gttcgggcag tgggacccga agaaccagcg tcccgagctg
     8341 tggaacctgt acaacgcgcg tagccacaag ggcgagaaca tccgcgcctt cccgatcagc
     8401 aactggaccg aactcgacgt gtggcagtac atccagcgcg agcagctgga gctgccatcg
     8461 atctacttcg cccacacccg cccggtggtg cgcaagaacg gcctgctgca gccggtgacc
     8521 gagctgacgc cggcgaagga tggcgacgtc gtggaggacg tgcgggtgcg cttccgcacc
     8581 gtgggggaca tcacctgcac ggcgccggtg gaatccgacg cggacaccgt ggagaagatc
     8641 ctcgccgaga cggcgaccac cacgatcacc gagcgcggtg cgacgcggat ggacgaccag
     8701 acctcggagg cgtcgatgga gcaacgcaag aaggaaggct acttctgagc gcatccgcgc
     8761 tcgtcgcagc ccccaccccc cactcttcgc acctcacgcc tgacctttgc acggaatcat
     8821 gaccatgtcc
//
LOCUS       DAOQBS010000127         8595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_7697_length_8595_cov_15.105621, whole genome shotgun sequence.
ACCESSION   DAOQBS010000127 DAOQBS010000000
VERSION     DAOQBS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7697_length_8595_cov_15.105621"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PKN25_12480"
     CDS             <1..1046
                     /locus_tag="PKN25_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNV91760.1"
                     /translation="PVDQYIGGIEHAILHLLYSRFFTRAMRDCGLVKVSEPFSALLTQ
                     GMVVAETYYRELDGGKKQWINPADVVVERDERGRIVAAKLESDGLPVVIGGTEKMSKS
                     KNNGVDPQSLVDQYGADTARLFMMFAAPPDQQLEWSDSGVEGASRFLRRVWNYGHTFA
                     TATRAALPATRALAAGTLPVAAADVRREVHTHLKQASYDFGKHQFNTVVSAAMKILNA
                     LEKAHKDLAGTEPAAHAEVAEEGFSILLRLLSPITPHICHALWQDCGFGTDILAAQWP
                     EVSEDALKRDEIELMLQVNGKLRGSLEVAADAAKADIEALALASEAAQKFMEGKPAKK
                     VVVVPGRLVNIVV"
     gene            1058..1585
                     /gene="lptE"
                     /locus_tag="PKN25_12485"
     CDS             1058..1585
                     /gene="lptE"
                     /locus_tag="PKN25_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNV91761.1"
                     /translation="MTAPGPVMATARRRLLGGALGAAALLLSGCGFRLRGPQALDFAT
                     VHISVPEQSEFGAQLRRLVATTGTTRVEEDAAKAEARLQILSNDRGREILSLTGAGKV
                     REYQLVQSLRFQLLDRAGKALIPPTSLTARREYTFDDSQVLGKEQEEALLYRDMQNDL
                     VQQLMRRLAAARRSG"
     gene            1605..2615
                     /gene="holA"
                     /locus_tag="PKN25_12490"
     CDS             1605..2615
                     /gene="holA"
                     /locus_tag="PKN25_12490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNV91762.1"
                     /translation="MNLRPDQLAAQLERPLAPLWLLHGNEPLLVLEAADAIRAAARRQ
                     GYEERETLVVGHGFKWDALALAAGNLSLFGGAKLIDLRIPTGKPGRDGGDALQRYIAH
                     LPSQTLTLVTLPEVDWATRKTAWFKALADAATTLELHAPERERLPEWVARRLAAQKQS
                     APPEALAFIADHVEGNLLAAHQEILKLGLLHGEGALTLEQVQDAVLDVARYDIDKLRL
                     AVVEGDPARCARLLEGLEGEGAAPPLVLWALAHETRSLATLSAGRDAGQPLAALFKAE
                     RIFDPRRQQAMTRALGRLGQGHLRAALMHAARIDRMIKGLASGDLWDEFLQLALRLAR
                     RG"
     gene            2725..4002
                     /locus_tag="PKN25_12495"
     CDS             2725..4002
                     /locus_tag="PKN25_12495"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNV91763.1"
                     /translation="MDIQHYMQTLGRQARAASRVLAAASTAAKNAALIAMAAEIRARR
                     AELLAANAQDLDEARAAGLEPALIDRLTLDEKGIESMAAGLEQVAALPDPVGEMSDIK
                     RRPSGIALGKMRVPLGVIGIIYEARPNVTADAAALCLKSGNAAILRGGKEAIHANRAI
                     AACVRAGLLAAGLPEHAVQVVEITDREAVGALITMPEFVDVIVPRGGKGLIERISRDA
                     RVPVIKHLDGNCHVYIDDEADPAKVVPIVENSKTQRYGTCNTAESLLVARDVADIYLP
                     EIARTLAAKGVEMRCCAESLALLREAGIAGAKLVEATAADWREEYLAPIIAVKIVAGL
                     DEAIEHINTCSSGHTEAIVTENYSHAMRFLREVDSSSVMVNASTRFADGFEYGLGAEI
                     GISTDKIHARGPVGLEGLTSQKWIVFGNGEVRR"
     gene            4038..4622
                     /locus_tag="PKN25_12500"
     CDS             4038..4622
                     /locus_tag="PKN25_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNV91764.1"
                     /translation="MSIRILRSLIVAALLSASLAQAAVKVGNVEFAPQQTVQQRTLEL
                     NGAGLRTRLGFKVYAMGLYLRAPLAEAGAILADKGEKRIRIVPIRDLEAKQFGDALQT
                     GLEKNHDDAALAALKPATDALLGALAAVGEVKAGTEIVLDRLGNGATRLLVDTKQQGE
                     DIADAAFYPALLRIWLGERPADSELREALLKNPQ"
     gene            4637..5125
                     /locus_tag="PKN25_12505"
     CDS             4637..5125
                     /locus_tag="PKN25_12505"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNV91765.1"
                     /translation="MTAGRAPASVFDAVIALPFGPFGIRAHDGQVQELVFLPPGTPLH
                     EPRNASAGAAAHALRAWIDDPDQPFPVPLAPCGTPFRRRVWTAIAAIPRGRTATYGEL
                     AASLDSAPRAVGQACGANPFPLIVPCHRVTSAAGLGGFANAREGWLLDVKRWLLAFEG
                     AL"
     gene            5122..6051
                     /gene="xerD"
                     /locus_tag="PKN25_12510"
     CDS             5122..6051
                     /gene="xerD"
                     /locus_tag="PKN25_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNV91766.1"
                     /translation="MSGRAPAREAGLQPAHRAELDRFADALWLEHGLARNTLAGYRSD
                     LARFAAWLETRAQRLPAAAGADLAAYLAEFSRAAKPASQRRLIAAWRRYYRLLLANRE
                     IAEDPTLAIDTPRAAERFPKTLSEPQVEALLAAPALDTPQGLRDRCMLEVLYAAGLRV
                     SELVGLKIFAVGVNEGVVRVMGKGSKERLVPLGEIAADWLQRYLAEARPVLLAGRSCD
                     EIFVTRLGAAMSRQMFWRLIKHYALAAGIAPERISPHTLRHAFATHLLNHGADLRVVQ
                     LLLGHADISTTQIYTHVARERLKQLHARHHPRA"
     gene            6167..6655
                     /gene="ybaK"
                     /locus_tag="PKN25_12515"
     CDS             6167..6655
                     /gene="ybaK"
                     /locus_tag="PKN25_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNV91767.1"
                     /translation="MSRNEAHAPETPATRFLRQHGVAFSSHLYEYEEHGGTTVSSREL
                     NVPEHAVVKTLIMEDENAQPMVVLMHGDHKVSTKELARQIPCKHVETCKPETANRHSG
                     YLVGGTSPFGTRKKMPVYMERTVLDLPLVYINGGRRGFLVGIHPHDLLRVLQPRLVQV
                     AN"
     gene            complement(6675..7049)
                     /locus_tag="PKN25_12520"
     CDS             complement(6675..7049)
                     /locus_tag="PKN25_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNV91768.1"
                     /translation="MDFIFIEELRVKAWVGIYAREKTGPQTVELNLTFGVPDAAAEHD
                     DIADTIDYAVVTERIRRELGERHFNLIESLGEFVVKLLFEEFGAPWVKLKVGKIGVMK
                     DVRRVGVYIQRSKAGVVVPPAA"
     gene            7217..7825
                     /gene="plsY"
                     /locus_tag="PKN25_12525"
     CDS             7217..7825
                     /gene="plsY"
                     /locus_tag="PKN25_12525"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNV91769.1"
                     /translation="MSTLLLIVAAYLLGSIPFAIVTSRLFGLEDPRKYGSGNPGATNV
                     LRSGNKAAAVLTLLGDALKGWLAVWAALRLGFEPDIAALAGLAAFLGHVFSLFLRFNG
                     GKGVATALGVLAGVDARIAIFCAVIWLMVAYTTRYSSAAALSAALAAPLAGLLFLGAK
                     PAVGLLAVMTAVLFWRHKENIARLRAGTEGKIGGKKKDGPAA"
     gene            complement(8043..>8595)
                     /locus_tag="PKN25_12530"
     CDS             complement(8043..>8595)
                     /locus_tag="PKN25_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584910.1"
                     /note="universal genome maintenance protein;
                     Kae1/Qri7/OSGEP/YgjD family protein; in Archaea, some Kae1
                     are found as fusion proteins similar to two distinct
                     proteins in yeast that are involved in the KEOPS complex;
                     kinase associated endopeptidase 1 (Kae1) and a
                     serine/threonine protein kinase (Bud32); in Pyrococcus
                     Kae1 has atypical AP endonuclease activity and inhibits
                     the kinase activity of Bud32; the protein is essential in
                     Escherichia coli and Bacillus subtilis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNV91770.1"
                     /translation="EAFDKTAKLLGLGYPGGPQLAGLAESGVPGRFRLPRPMLHSGDL
                     DFSFSGLKTAVLNVVSAPDWDPARMADLAAEFQQAVVDVLCAKALAALKKVGLKTLVV
                     AGGVGANRCLRATLDAALARRGGRVHYPEPALCTDNGAMIAFAGALRLAAGESVPEVC
                     AVRIRPRWPMVELRPPVQAPAIL"
BASE COUNT         1294 a         3267 c         2866 g         1168 t
ORIGIN      
        1 cgccggtcga ccagtacatc ggcggcatcg agcacgccat cctgcacctg ctgtactcgc
       61 gcttcttcac ccgcgccatg cgcgactgcg gcctggtgaa ggtctccgag cccttcagcg
      121 cgctgctcac ccagggcatg gtggtggccg agacctatta ccgcgagctc gacggcggca
      181 agaagcagtg gatcaacccg gccgacgtgg tcgtcgagcg tgacgagcgc ggccgcatcg
      241 tcgccgcgaa actcgaatcc gacggcctgc cggtggtcat cggcggcacc gagaagatgt
      301 cgaagtcgaa gaacaacggc gtcgacccgc agtcgctggt ggaccagtac ggcgccgaca
      361 ccgcgcgtct cttcatgatg ttcgccgcgc cgcccgacca gcagctcgag tggtccgact
      421 ccggcgtcga gggcgcttcg cgcttcctgc gccgggtgtg gaactacggc cacaccttcg
      481 ccaccgccac gcgcgccgcg ctgccggcca cgcgcgcgct cgccgccggc acgctgcccg
      541 tcgccgcggc cgacgtgcgc cgcgaggtgc acacccacct caagcaggcg agctacgact
      601 tcggcaagca ccagttcaac acggtggtgt cggcggcgat gaagatcctc aacgcgctcg
      661 agaaggcgca caaggatctg gccggcaccg agccggcggc gcacgccgag gtcgccgaag
      721 aaggcttctc catcctgctg cgcctcctgt cgccgatcac cccgcacatc tgccacgcgc
      781 tgtggcagga ctgcggcttc ggcaccgaca tcctggcggc gcagtggccc gaggtcagcg
      841 aggacgcgct gaagcgggac gagatcgagc tgatgctgca ggtcaacggc aagctgcgcg
      901 gcagcctcga ggtcgcggcc gacgcggcga aggccgacat cgaggcgctc gccctcgctt
      961 cggaagcggc gcagaagttc atggagggca agccggcgaa gaaggtggtc gtcgtgcccg
     1021 gccgcctggt caacatcgtc gtttgaggaa gcgcggcatg actgctcctg gtcccgtcat
     1081 ggccaccgcg cggcggcgcc tgctcggtgg cgccctcggt gccgcggcgc tgctgctgtc
     1141 gggctgtggc tttcgcctgc gcggcccgca ggcgctcgac tttgcgaccg tgcacatcag
     1201 cgtgccggag cagagcgaat tcggcgccca gctgcgtcgc ctcgtcgcca ccaccggcac
     1261 cacccgggtc gaggaggacg cagccaaggc cgaagcccgc ctgcagatcc tctccaacga
     1321 ccgcggccgc gagatcctca gcctcaccgg tgcgggcaag gtgcgcgagt accagctcgt
     1381 ccagtccttg cgcttccagc tcctcgaccg cgcgggcaag gcgctgatcc cgcccaccag
     1441 cctgacggcg cggcgcgagt acaccttcga cgacagtcag gtgctcggca aggaacagga
     1501 agaggccttg ctctaccgcg acatgcagaa cgacctggtg cagcagctca tgcgccgcct
     1561 cgccgccgcc cgccgcagcg gctgaacccg ttccgccgcc ccgcgtgaac ctgcgccccg
     1621 accagctcgc cgcccagctc gagcgcccgc tcgcgccgct ctggctgctg cacggcaacg
     1681 agccgctgct ggtgctcgag gctgccgacg caatccgcgc cgcagcccgt cgccagggct
     1741 acgaggagcg cgagaccctg gtcgtcggcc acggcttcaa gtgggacgcg ctcgccctcg
     1801 ccgcgggcaa cctgtcgctc ttcggcggcg ccaagctgat cgacctgcgc atcccgacgg
     1861 gcaagcccgg ccgcgacggc ggcgatgcgc tgcagcgcta catcgcccac ctgccttccc
     1921 agaccctgac cctggtcacc ctgccggaag tcgattgggc gacgcgcaag accgcctggt
     1981 tcaaggccct ggccgacgcc gcgacgacgc tggagctcca cgcccccgaa cgcgaacgtc
     2041 tgcccgagtg ggtcgcgcgc cggctcgccg cgcagaagca gtccgccccg cccgaggcgc
     2101 tcgccttcat tgccgatcac gtcgaaggca acctgctcgc cgcccaccag gagatcctca
     2161 agctcggcct gctgcacggc gagggcgcgc tcacgctcga gcaggtgcag gacgcggtgc
     2221 tcgacgtcgc ccgctacgac atcgacaagc tgcgcctggc ggtcgtcgag ggcgacccgg
     2281 cgcgctgcgc ccgcctgctc gaaggcctcg agggtgaggg cgccgccccc ccgctggtgc
     2341 tgtgggcgct cgcgcacgag acacgcagcc tcgccaccct gagcgcgggc cgcgacgccg
     2401 gccagcccct ggccgccctg ttcaaggccg agcgcatctt cgatccgcgc cgccagcagg
     2461 ccatgacccg tgccctcggt cgcctcggcc agggccatct gcgcgccgcg ctgatgcacg
     2521 cggcgcgcat cgaccgcatg atcaagggcc tcgccagcgg cgacctgtgg gacgagttcc
     2581 tccagctcgc gctgcgcctg gcgcgccgcg gctgaaccgc ggccgacccg cgccaggctt
     2641 catccgcatc cggttcgatg atttaatgcg gatttgcccc gcggcgtccg tggatgccgc
     2701 gaccgacgcc gaagaagagc ccccatggac atccagcact acatgcagac cctgggccgc
     2761 caggcccgcg ccgcctcgcg cgtgctcgcc gccgcctcca ccgcggccaa gaacgccgcc
     2821 ctgatcgcga tggccgccga gatccgcgcc cgccgcgccg aactgctcgc cgccaatgcc
     2881 caagacctcg acgaggcgcg tgccgccggc ctggagccgg cgctgatcga ccgcctgacg
     2941 ctggacgaga aaggcatcga gtccatggcc gccggcctcg agcaggtcgc cgcgctgccc
     3001 gacccggtgg gcgagatgag cgacatcaag cgccgcccct ccggcatcgc gctcggcaag
     3061 atgcgcgtgc cgctgggcgt gatcggcatc atctacgagg cccgccccaa cgtcaccgcc
     3121 gacgccgcgg cgctgtgcct caaatccggc aatgccgcga tcctgcgcgg cggcaaggag
     3181 gcgatccacg ccaaccgcgc gatcgccgcc tgcgtgcgcg ccggcctgct ggcggccggt
     3241 ctgcccgaac acgcggtgca ggtggtcgag atcaccgacc gcgaggccgt cggtgcgctg
     3301 atcaccatgc ccgagttcgt cgacgtgatc gtgccgcgcg ggggcaaggg cctgatcgag
     3361 cgcatctcca gggacgcgcg cgtgccggtg atcaagcacc tcgacggcaa ctgccatgtt
     3421 tatatcgacg atgaggccga ccctgccaag gtcgtaccca tcgtcgagaa ctccaagacc
     3481 cagcgctatg gcacctgcaa caccgccgaa tcgctgctgg tggcgcgcga cgtcgccgac
     3541 atctacctgc ccgagatcgc ccgcacgctc gccgccaagg gcgtggagat gcgctgctgc
     3601 gccgagtcgc tcgccctcct gcgcgaggcc ggcatcgccg gggcgaagct ggtggaggcg
     3661 accgcggccg actggcgcga ggaatacctc gccccgatca tcgcggtgaa gatcgtcgcc
     3721 ggcctcgacg aggcgatcga gcacatcaac acctgcagtt cgggccacac cgaagcgatc
     3781 gtcaccgaga actattccca cgccatgcgc ttcctgcgcg aggtagattc atcctcggtg
     3841 atggtgaatg cctccacccg cttcgccgac ggcttcgagt acggcctcgg cgcggagatc
     3901 ggcatctcca ccgacaagat ccacgcccgc ggcccggtcg gcctggaggg ccttaccagc
     3961 cagaaatgga tcgtgttcgg caacggcgaa gtccgccgct gacactgcca aatggaacca
     4021 ccccgacaag gaaccccatg agcatccgca tcctccgcag cctgatcgtc gccgccctgc
     4081 tctcggccag cctcgcccag gccgccgtca aggtcggcaa cgtcgagttc gccccgcagc
     4141 agaccgtgca gcagcgcacg ctcgagctca acggcgccgg cctgcgcacc cggctcggct
     4201 tcaaggtcta tgcgatgggc ctgtacctgc gcgccccgct cgccgaggcc ggcgcgatcc
     4261 tggccgacaa aggcgagaag cgcatccgca tcgtgccgat ccgcgacctc gaggcgaagc
     4321 agttcggcga tgccctgcag accgggctcg agaagaacca cgacgacgcc gcgctggccg
     4381 cgctcaagcc cgccaccgac gcgctgctgg gcgcgctcgc cgcggtcggc gaggtcaagg
     4441 ccggcaccga gatcgtgctc gatcgcctcg gcaacggcgc cacccggctg ctggtcgata
     4501 cgaagcagca gggcgaggac atcgccgacg ccgccttcta tcccgcgctg ctgcgcatct
     4561 ggctgggcga gcgcccggcc gacagcgagc tgcgcgaggc gctgctcaag aatccgcagt
     4621 gaggcaggaa ctcgccatga cggcgggccg tgctcccgcg tccgtattcg acgccgtgat
     4681 cgcgctgccc ttcggcccct tcggcatccg cgcgcacgac gggcaggtgc aggagctcgt
     4741 cttcctgcct ccgggcacgc ccttgcacga accgcgcaac gccagcgccg gcgccgcggc
     4801 gcacgcgctg cgcgcctgga tcgacgaccc cgaccagccc ttcccggtgc cgctcgcacc
     4861 ctgcggcacg cccttccgtc gtcgcgtatg gacggccatc gcggcaatcc cgcgcggacg
     4921 taccgcaacc tacggcgagc tcgccgccag cctggacagc gcgccgcgcg cggtcggcca
     4981 ggcctgcggc gccaacccct ttcccctgat cgtgccctgc caccgcgtga cctcggccgc
     5041 cgggctcggt ggcttcgcca acgcccgcga aggctggctc ctcgacgtca agcgctggct
     5101 gctcgctttc gaaggggcgc tgtgagcgga cgcgcgccgg cacgcgaagc cggcctgcag
     5161 cccgcgcacc gcgccgagct cgaccgcttc gccgatgcgc tctggctcga gcacgggctg
     5221 gccaggaaca ccctcgccgg ctaccgcagc gacctcgccc gcttcgccgc ctggctggag
     5281 acgcgtgcgc aacgcctgcc cgccgccgcc ggcgcggacc tcgccgccta cctcgccgaa
     5341 ttcagccgcg ccgcgaagcc ggcgagccaa cgccggctga tcgccgcgtg gcggcgctat
     5401 taccgcctgc tcctcgccaa ccgcgagatc gccgaggacc ccacgctggc gatcgacacc
     5461 ccgcgcgcgg ccgaacgctt tcccaagacc ctgagcgagc cccaggtcga ggccctgctc
     5521 gccgctcccg cgctcgacac cccgcaaggc ctgcgcgacc gctgcatgct ggaggtgctc
     5581 tacgccgccg gcctgcgggt ctccgagctg gtcggcctga agatcttcgc ggtgggggtg
     5641 aatgagggcg tggtgcgggt catgggcaag ggcagcaagg agcgcctggt gccgctcggc
     5701 gagatcgccg ccgactggct gcagcgctac ctggccgagg cacgcccggt cctgctcgcc
     5761 ggccgcagct gcgacgagat cttcgtgacc cgcctggggg cggcgatgtc gcgccagatg
     5821 ttctggcgcc tgatcaagca ctacgccctc gccgccggca tcgcgcccga gcgcatctcg
     5881 ccccacaccc tgcgccacgc cttcgccacc cacctgctca accacggcgc cgacctgcgc
     5941 gtggtgcagc tgctgctcgg ccacgccgac atctcgacca cgcagatcta cacccacgtc
     6001 gcgcgcgagc gcctcaagca gctgcacgcg cgccaccatc cgcgcgcatg agcgcgccgg
     6061 ccacggccgg ggcgacgcgt ccatacgccc cggcatggcg gatggcacta caatgtggag
     6121 accgcgccgc ccgctcgcgg cggcgcgccc gacaggccgt ccgatcatga gcagaaacga
     6181 agcccacgcc cccgagaccc ccgccacccg cttcctgcgc cagcacggcg tcgccttctc
     6241 cagccacctc tacgaatacg aggagcacgg cggcaccacg gtgtcctcgc gcgaactcaa
     6301 cgtgcccgag cacgcggtgg tcaagaccct gatcatggag gacgagaacg cgcagccgat
     6361 ggtggtgctg atgcatggcg accacaaggt gtccaccaag gagctcgcgc gccagatccc
     6421 gtgcaagcac gtcgagacct gcaagccgga gaccgcgaac cgccacagcg gctatctggt
     6481 gggcggcacc tcgcccttcg gcacgcgcaa gaagatgccg gtgtacatgg agcgcaccgt
     6541 gctcgacctg ccgctggtct acatcaacgg cgggcggcgc ggcttcctgg tcgggatcca
     6601 cccgcacgac ctgctgcgcg tgctccagcc caggctggtg caggtggcga actgaagcga
     6661 gcgcccgcgg gcgctcaggc ggccggcggt acgaccacac ccgccttgct gcgctggata
     6721 tagacgccga cgcggcgcac atccttcatc accccgatct tgcccacctt gagcttgacc
     6781 cagggcgcgc cgaactcctc gaacagcagc ttgacgacga actcgcccag actctcgatg
     6841 aggttgaagt ggcgctcgcc gagctcgcgg cgaatgcgct cggtcaccac cgcatagtcg
     6901 atggtgtcgg cgatgtcgtc gtgctctgcg gcggcgtcgg gaacgccgaa ggtgaggttc
     6961 agctcgaccg tctgcgggcc ggtcttctcg cgcgcgtaga tgccgaccca ggccttgacc
     7021 cgcagttcct cgatgaagat gaaatccatg ctgcttcccg tttagaatcg tccggattct
     7081 aacgcgcccc cgcgcgcgcg cccggaaaag ctgtcgcaca ccatcggtgc cggcttgcga
     7141 caggcgaggc gtgccggatg ccccctcccc ccttccgtcc ctttcgagct gcccgccggc
     7201 gggcagtgga gccaagatgt ccaccctgct tctgatcgtc gccgcctacc tgctcggctc
     7261 cattcccttc gcgatcgtca ccagccgcct gttcggcctg gaagatccgc gcaagtacgg
     7321 ctcgggcaac cccggcgcga ccaacgtgct gcgcagcggc aacaaggccg cggccgtgct
     7381 caccctgctc ggcgacgcgc tcaagggctg gctggcggtg tgggcggcgc tgcgcctcgg
     7441 cttcgagccg gacatcgccg cgctcgccgg gctggccgcc ttcctcggcc acgtgttctc
     7501 gctcttcctg cgcttcaacg gcggcaaggg cgtggccacc gcgctcggcg tactcgccgg
     7561 ggtcgacgca cgcatcgcga tcttctgcgc ggtgatctgg ctgatggtgg cctacaccac
     7621 gcgctactcc tcggccgcgg cgctgtcggc ggcgctcgcc gcgcccctcg cgggcctgct
     7681 cttcctcggc gccaagccgg cggtcggcct gctcgcggtg atgacggcgg tgctgttctg
     7741 gcgccacaag gagaacatcg cccgcctgcg cgccggcacc gagggcaaga tcggcggcaa
     7801 gaagaaggac gggccggcgg cgtaaggcct ccgggcgcgc ggaggtccat gcaggagcgc
     7861 ccgcaggcgc gaatgcgggg gaggagcggc ggctctgatg ccgggcgccg ggttcgcggc
     7921 ttgcgccgct cctacaaagg gaagccgcaa cgtttcctgt gggagcgccg ccaggcgcga
     7981 atacggcggt ggagcggcgg cggctctgat gccgggcgcc gggttcgcgg cttgcgccgc
     8041 tcctacagga tggcgggcgc ctgcaccggc gggcgcagtt cgaccatggg ccagcgcggg
     8101 cggatgcgga cggcgcagac ttccggcacg gactcgccgg ccgccaggcg caaggcgccg
     8161 gcgaaggcga tcatggcgcc gttgtcggtg cacagcgccg gctcggggta atgcacgcgc
     8221 ccgccgcggc gcgcgagcgc ggcgtccagg gtggcgcgca ggcagcggtt ggcacccacg
     8281 ccgccggcca ccacgagggt cttcagcccc accttcttca gcgccgcgag cgccttcgcg
     8341 cacagcacat cgaccacggc ctgctgaaac tcggcagcga ggtcggccat gcgcgccggg
     8401 tcccagtcgg gcgccgacac cacgttgagc accgcggtct tgagaccgct gaagctgaag
     8461 tcgaggtccc ccgagtgcag catcggccgc ggcaggcgga agcggccggg cacgccggat
     8521 tcggccagcc cggcgagctg cggcccgccg ggatagccca ggccgagcag cttggcggtc
     8581 ttgtcgaagg cctcg
//
LOCUS       DAOQBS010000128         8538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_7809_length_8538_cov_14.611458, whole genome shotgun sequence.
ACCESSION   DAOQBS010000128 DAOQBS010000000
VERSION     DAOQBS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7809_length_8538_cov_14.611458"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKN25_12535"
     CDS             <1..628
                     /locus_tag="PKN25_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNV91771.1"
                     /translation="ENFVLPLSHDEVVHGKGSLLAKMPGDVWQKFANLRLLYGYMWAH
                     PGKKLLFMGCEFAPWSEWNADASLPWELLGHAPHAGMQRLVRDLNRVLRSHPALYEQD
                     TASEGFRWISHDDAAHSVLVFERRARDGRSVVVACNFTPVAREGWRIGVPAAGTWREL
                     LNTDDPVYGGSGVTNGALPSAALPWQGEAQSIVLRLPPLGVAMFVRVD"
     gene            666..2975
                     /gene="glgX"
                     /locus_tag="PKN25_12540"
     CDS             666..2975
                     /gene="glgX"
                     /locus_tag="PKN25_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNV91772.1"
                     /translation="MSAPHPDSLPILQHGCAPCEDGPGAAADPLAMAHALPDPDLRPG
                     RPWPLGATLTADSGETGVNFAVWSPDAEAIEVCLFDAAGREALRRLALPACSEGVWHG
                     FLPAGGVGLVYGLRAHGPWAPGRGHWFNPAKLLLDPWAQEVVGSYGRQQPGAADDVEL
                     AARLAAFRAARSDDPRLPDPRDNAAFAPKARVPAPAPAPAYDRPPRPHIPRERTVLYE
                     AHVRALTMRHPDIPPALRGSYAALAHPVMLDHYRALGLTTLSLLPLHFRADEAALQRR
                     GLANHWGYAPAAWLAPEPRYWSGRAGTTPTGELAGAIDALHAAGLEVVLDVVFNHTAE
                     LDADGPMLSLRGLANARAYHLDPQDPACYRDWSGCGNAVNLGEPRMVELVVGALRHWA
                     ARYRIDGFRLDLAVTTGRDRHGHFDTGAPLFAAIAADPLLARLKWIAEPWDLGPDGYR
                     LGGFAPGWMEWNDRFRDTMRAWWLCGGGDRGAFAHRFAGSAAEFDHDRRAPTASINFL
                     CAHDGFGLRDVVSHDHRHNEANGEHNRDGHPHNHSWNCGVEGPSADPDVLALRARLQR
                     ALLASLLLAQGTPMLLAGDEIGHSQRGNNNAYCQDNETTWLDWAAADRELLACARRLL
                     ALRAAHPALRQVAWLRGPEHGEAASVRWLRPDGAELQAADWNDPRERALAILLHPPGL
                     RPHTVEHIAKPAAAAPARSVLLLVNPAAAACRFRLPAGGWSPVFDSARADGGPAPPAP
                     GHESHPVEPVLSARTVLVLLESAQHHQES"
     gene            2978..4246
                     /gene="glgC"
                     /locus_tag="PKN25_12545"
     CDS             2978..4246
                     /gene="glgC"
                     /locus_tag="PKN25_12545"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNV91773.1"
                     /translation="MPAEAANRRRILTRRTLALVLAGGRGSRLRDLTNVRAKPAVHFG
                     GKFRIIDFALSNCMNSGLRRIGVITQYKSHSLLRHLQRGWSFLRNEMGEFVDLLPAQQ
                     RIDEEQWYQGTADAVFQNLDIIRNSTPPDYIVVLAGDHVYKMDYSIMLEDHAASGRGV
                     TVGCIEVPREEAKAFGVMAIDARRHITAFVEKPADPPALPGNPGLSLASMGIYIFSAN
                     YLYRLLEDDAKNPDSSHDFGKDLIPRAVAENQALAHPFTLSAIATPPFSGPYWRDVGT
                     VDAYWAANLDLASTTPALNMYDKDWPIWTYQEQLPPAKFVHDLDGRRGEALNALVSGG
                     CIVSGSVVRESVLFSNVLVRSYSTIEQAVVLPDVQINRHCRLKKVVIDRHCVIPERTV
                     IGEDAEADARRFHRTEGGVVLVTREMLDRL"
     gene            4263..5723
                     /gene="glgA"
                     /locus_tag="PKN25_12550"
     CDS             4263..5723
                     /gene="glgA"
                     /locus_tag="PKN25_12550"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNV91774.1"
                     /translation="MRILQVCAEIFPLLKTGGLADVAGALPPALGALGADVRVLLPGF
                     DAILAGLESPVEIARLQAPGGMGNNPARLLRGHLPAIGGEAYVIDAPGWYLRTGGPYA
                     DAAQQPYADNHLRFALLGWTAAELAQGLDTSWQPGVVHAHDWHAGLVPAWLQVAAARG
                     RSVPGCVFTVHNLAYQGMFPRACFDALGLPAHFFSIHGVEFHGHLNFMKAGLYYAGRV
                     TTVSPSYAREIQGPEQGCGLDGLLRARAHELSGILNGVDAAVWDPATDTLLPARYTAD
                     YMAGKARCRSALRRELGLAGKAEGPLYCVVSRLTEQKGLHLVLQALPALLAGGGQLAL
                     LGSGDAGLEAAFRDAAAAAPESVAVRIGYDEAFAHRLIAGSDVILVPSHYEPCGLTQL
                     YGLKYGTLPLVRRVGGLADTVADTRLETLDTDATGFVFEEFSAAALERTLLRVNAVHR
                     RPTEWRRVRARGMAKDFGWARAAREYLALYRGLTPA"
     gene            5909..7363
                     /locus_tag="PKN25_12555"
     CDS             5909..7363
                     /locus_tag="PKN25_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV91775.1"
                     /translation="MAALALGAGSAQAAGIDAGGLAWNGGLVLLGGGLGYLLGRRSLR
                     RRTLAGAAEGCAADGPLHDDAPASGGAVSRREWAGTPADADDCAPASALPHALLPAAH
                     AAPPAGAGAAIGVIATESYREVVDSLSEVLFRTDELGRLVFLNDAWKDLSGFDLDATR
                     QHALTDFLHPDDRLRARDAIRALLCGDGQEWTDELRLRTLSGEIRWVAIDCRALRDGS
                     GQESGIAGTIDDISARKIAEFSLRNLNQELESRVRSRTAELETAVRELEAFSYSVSHD
                     LRAPLRAIDGFARILVEEAGPRLDERQREQLVRIRAGAERMAILIDALIDLASVSRQP
                     LRRRPVDLSRIAEAVIRDLQAESPGRVVAVEITSDMTVVADPVLMHVLLDNLLRNAWK
                     FTSQCEHPRIVFGAERDGERTVFHVEDNGAGFEMNYAGKLFQPFQRLHAQHEFPGTGI
                     GLATVQRIVARHEGRVWASAEPGKGARFCFMLGH"
     gene            7562..>8538
                     /locus_tag="PKN25_12560"
     CDS             7562..>8538
                     /locus_tag="PKN25_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNV91776.1"
                     /translation="MKEKHYLTPLLEPRSVGIIGASEREASLGSVLMRNMLEAGYKGK
                     LFAINPKHEKVHGVACYKSVEDVPQRLDLVVMAIRAEKTPALMEACGRAGVKAVILLS
                     GGFSESGARGALLERQVVEAAHRHRIRLLGPNCLGVMRPQLGVNATFAHASALKGSIG
                     LISQSGALCAAILDWAKPNNVGFSTVVSLGSSSDIDFGEVLEYMISDPRTESIFLYVE
                     GIRDARRFMSALRGAARVKPVLLVKAGRHPGASRAILSHSGAPMGEDAVFDAALRRAG
                     VIRLYNMGQLFAAANALFSHFRPRGNRLAIITNGGGPGVMAADRAADIG"
BASE COUNT         1233 a         3196 c         2899 g         1210 t
ORIGIN      
        1 cgagaacttc gtgctgccgc tgtcgcacga cgaggtggtg cacggcaagg gctcgctgct
       61 cgccaagatg cccggcgacg tctggcagaa gttcgccaac ctgcgcctgc tctacggcta
      121 catgtgggcg cacccgggca agaagctgct cttcatgggt tgcgagttcg ccccgtggtc
      181 cgaatggaac gccgacgcca gcctgccctg ggagctgctc gggcacgccc cgcacgcggg
      241 catgcagcgc ctggtgcgcg atctcaaccg cgtgctgcgc agccatccgg cgctgtacga
      301 gcaggacacg gcgagcgaag gcttccgctg gatctcccac gacgatgcgg cgcactcggt
      361 gctggtcttc gagcgccgcg cgcgcgacgg ccgcagcgtg gtggtcgcgt gcaacttcac
      421 cccggtggcg cgcgagggct ggcgcatcgg cgtacccgcg gcgggcacct ggcgcgagct
      481 tctcaacacc gacgatccgg tgtatggggg ctcgggcgtc accaatggcg cgctgcccag
      541 cgcggcgctg ccctggcagg gcgaggcaca gtcgatcgtg ctgcgcctgc cgccgctggg
      601 cgtggcgatg ttcgtacgcg tggactgaca cgcctggacg catgccccgg caagagaacg
      661 cagcgatgag cgccccgcac cccgactccc ttcccatcct gcagcacggg tgcgctcctt
      721 gcgaggacgg gcccggcgcc gcagcggatc cgctcgccat ggcgcatgcg ctgcctgatc
      781 cggacttgcg cccggggcgg ccgtggccgc tcggtgccac cctcaccgca gacagcggcg
      841 agaccggggt gaacttcgcg gtgtggtcgc cggacgccga agcgatcgag gtctgtcttt
      901 tcgatgccgc aggccgcgag gcgcttcgcc gcctggcgct gccggcgtgc agcgaaggcg
      961 tctggcatgg ctttttgcct gccggcgggg tcggcctggt gtatggcctg cgtgctcatg
     1021 gcccgtgggc gccagggcgc ggccactggt tcaacccggc gaagctgctg ctcgacccct
     1081 gggcgcagga ggtggtcggc agctacggcc gccagcagcc cggagcagcc gacgatgtgg
     1141 agctcgcggc aaggctcgcc gcgttccgcg ccgcccgcag cgacgatccg cgcctgcccg
     1201 acccgcgcga caacgctgcc ttcgccccca aggcgagggt gcccgccccc gcccctgctc
     1261 cggcgtatga ccgccctccc cgcccgcaca tcccgcgcga gcgcacggta ctctacgagg
     1321 cccacgtgcg tgcgctgacg atgcgccacc cggacatccc gccggcgctg cgcggcagct
     1381 acgccgccct cgcccacccg gtgatgctcg atcactaccg ggcgctcggc ctcaccacgc
     1441 tgagcctgct gcccctgcac ttccgcgccg acgaagcggc gctgcagcgc cgtggcctcg
     1501 ccaaccactg gggctatgcg cccgcagcct ggctcgcccc cgagccgcgc tactggagcg
     1561 ggcgcgcggg caccaccccg accggggaac tcgcgggggc gatcgacgcg ctgcacgccg
     1621 ccgggctcga ggtggtgctc gacgtggtct tcaaccacac ggccgagctc gacgcggacg
     1681 gcccgatgct gtcgctgcgc gggctggcca acgcgcgcgc ctaccatctc gacccgcaag
     1741 acccggcgtg ctaccgcgac tggagcggct gcggcaacgc ggtgaacctc ggcgagccgc
     1801 gcatggtcga gctggtcgtc ggcgcgctgc gccactgggc cgcgcgctac cgcatcgacg
     1861 gcttccgcct cgacctcgcg gtcacgaccg ggcgcgaccg ccacggccat ttcgataccg
     1921 gtgcgccgct gttcgcggcg atcgccgccg acccgctgct cgcccgcctg aagtggatcg
     1981 ccgaaccgtg ggacctcggc ccggacggct accggctggg cgggttcgcg cccggctgga
     2041 tggaatggaa cgaccgcttc cgcgacacga tgcgcgcctg gtggctatgc ggagggggag
     2101 accgcggcgc attcgcccac cgcttcgccg gttctgcggc ggagttcgac cacgaccgcc
     2161 gcgcccccac ggccagcatc aacttcctgt gcgcgcacga cggcttcggc ctgcgcgacg
     2221 tggtgagcca cgaccaccgc cataacgagg ccaacggcga gcacaaccgc gacggccatc
     2281 cgcacaacca ctcgtggaac tgcggcgtgg aaggcccgag cgcggacccc gacgtgctcg
     2341 ccctgcgcgc ccgcctgcag cgcgccctgc tcgccagcct gctgctcgcc cagggcacgc
     2401 cgatgctgct cgccggcgac gagatcggcc acagccagcg cggcaacaac aacgcctact
     2461 gccaggacaa cgagacgacc tggctcgact gggccgccgc cgaccgcgaa ctcctcgcct
     2521 gcgctcgaag gctgctcgcc ctgcgcgcag cccaccccgc gctgcgccag gtcgcctggc
     2581 tgcgcggccc ggagcacggc gaggcggcga gcgtgcgctg gctgcgcccc gatggcgccg
     2641 agctgcaggc cgccgactgg aacgatcccc gcgagcgcgc actggcgatc ctgctgcatc
     2701 cgcccggcct gcggccgcac acggtcgagc acatcgccaa acccgcggcc gccgcacccg
     2761 cgcgcagcgt gctgctgctc gtcaatcccg ccgcggccgc gtgccgcttc cgcctgcccg
     2821 cgggcggatg gtcgccggtc ttcgacagtg cccgggccga cgggggcccg gcgccgcccg
     2881 cccccggcca cgaaagccat cctgtcgagc cggtgctgtc cgcgcgcacc gtgctcgtcc
     2941 tcctagaatc cgcacaacac caccaggagt cctgatcatg cctgccgaag ccgccaaccg
     3001 acgacggatc ctcacgcgcc gcaccctcgc cctcgtgctc gccggcgggc gcggctcgcg
     3061 cctgcgcgat ctcaccaacg tgcgcgccaa gccggcggtg cacttcggcg gcaagttccg
     3121 catcatcgac ttcgcgctgt cgaactgcat gaactcgggt ctgcgccgca tcggcgtgat
     3181 cacacagtac aagtcgcact cgctgctgcg ccacctgcag cgcggctgga gcttcctgcg
     3241 caacgagatg ggcgaattcg tcgacctgct gccggcgcag cagcgcatcg acgaggaaca
     3301 gtggtaccag ggcaccgccg acgcggtgtt ccagaacctc gacatcatcc gcaactccac
     3361 gccgcccgac tacatcgtcg tgctcgccgg cgaccatgtg tacaagatgg actactcgat
     3421 catgctcgag gaccacgccg cgagcgggcg cggtgtcacc gtgggctgca tcgaggtacc
     3481 gcgcgaggag gccaaggcct tcggcgtgat ggcgatcgat gcacggcgcc acatcaccgc
     3541 cttcgtcgag aagcccgccg acccgccagc gctgccgggc aatcccgggc tgtcgctcgc
     3601 cagcatgggc atctacatat tctcggccaa ctacctctac cgcctgctcg aggacgacgc
     3661 gaagaacccg gactccagcc acgacttcgg caaggacctg atcccgcgcg cggtggcgga
     3721 aaaccaggcg ctcgcccacc ccttcacgct gtcggcgatc gccaccccgc ccttctccgg
     3781 cccctactgg cgcgacgtcg gcacggtgga cgcctactgg gcggccaacc tcgacctcgc
     3841 ctcgaccacg ccggcgctca acatgtacga caaggactgg ccgatctgga cctaccagga
     3901 gcaactaccg ccggccaagt tcgtgcacga tctcgacggt cgccgcggcg aggcgctcaa
     3961 cgcgctggtc tcgggcggct gcatcgtctc cggatcggtc gtgcgcgagt cggtgctgtt
     4021 ctccaacgtg ctggtgcgct cctacagcac gatcgagcag gcggtggtgc tgcccgacgt
     4081 gcagatcaac cgccactgcc gcctgaagaa ggtcgtcatc gatcgccact gcgtgatccc
     4141 cgagcgcacg gtgatcggcg aggacgccga ggcggatgcg cgccgcttcc accgcaccga
     4201 gggcggcgtg gtgctggtga cgcgcgaaat gctcgaccga ctgtgagcgc ggagaccaga
     4261 cgatgcggat ccttcaggtg tgtgccgaga tcttcccact gctgaaaacc ggcgggctgg
     4321 ccgacgtcgc cggggcgctg ccgcccgccc tgggcgcgct cggcgcggac gtccgggtgc
     4381 tgctgcccgg cttcgacgcc atcctcgccg ggctggagtc gccggtcgag atcgcccgcc
     4441 tgcaagcgcc gggcgggatg gggaacaacc cggcgcggct cctgcgcgga cacctgcccg
     4501 cgatcggcgg ggaggcctac gtgatcgacg ccccgggctg gtacttgcgc acgggtggtc
     4561 cctacgccga cgccgcacag cagccctacg ccgacaacca cctgcgcttc gccctgctcg
     4621 ggtggaccgc agccgagctc gcccaagggc tcgacacgtc ctggcaaccc ggcgtggtgc
     4681 acgcgcacga ctggcacgcc ggcctcgtgc cggcatggtt gcaggtcgcg gcagcgcgcg
     4741 ggcggagcgt gccgggctgc gtgttcacgg tacataacct cgcctatcag ggcatgttcc
     4801 cgcgcgcctg cttcgacgcg ctcgggctgc ccgcgcattt cttctcgatc cacggcgtgg
     4861 agttccacgg gcacctcaac ttcatgaagg ccgggctgta ctacgccggg cgcgtcacca
     4921 cggtgagccc gagctatgcg cgcgagatcc aggggccgga acagggctgc gggctggacg
     4981 gcctcctgcg cgcccgcgcg cacgagctct cgggcatcct caacggggtc gatgcggcgg
     5041 tgtgggatcc ggccacggat accctgctgc ccgcgcgcta caccgcggac tacatggccg
     5101 gcaaggcgcg ctgccgcagc gcgctgcgcc gcgaactcgg gctggcgggc aaggccgagg
     5161 gcccgttgta ctgcgtggtg agccggctca ccgagcagaa gggcctgcac ctggtgctgc
     5221 aggccctgcc cgcgctgctg gcgggcggcg gccagctcgc cctgctcggc agcggcgacg
     5281 ccgggctgga ggccgccttc cgtgacgcgg cggcagccgc gcctgaatcg gtggcggtgc
     5341 gcatcggcta cgacgaagcc ttcgcccacc gcctgatcgc cggcagcgac gtgatcctgg
     5401 tgccctcgca ctacgaaccc tgcggcctga cccagctcta cggcctcaag tacggcaccc
     5461 tgcctctggt gcggcgcgtc ggcggcctcg ccgacacggt agccgacacc cgcctggaga
     5521 cgctcgacac cgacgccacc ggcttcgtct tcgaggagtt ctccgccgct gcgctcgaac
     5581 gcacgctgct gcgggtgaat gccgtccacc gccgtcccac cgaatggaga cgcgtgcgcg
     5641 cccgcggcat ggcgaaggac ttcggctggg cgcgtgccgc gcgggaatat ctcgcccttt
     5701 atcgtggtct cacgcctgca tgaggtcgcg ccggcgcccg tcgccgcacc cgcttcgcgc
     5761 acggcccgcc gcgggcagct tctgtgctat acaggacgag tccgaccgcc acggaaacca
     5821 tgcggcatgc gggaccgccg ccactgacca ggtgagcccc atcgacgcgt ccagaccgca
     5881 cccaccgcct cgtgccgggc atgtgctcgt ggcggcgctc gccctcggcg cgggctcggc
     5941 gcaggccgcg gggatcgatg ccggcgggct ggcctggaat ggcggtctgg ttctgctcgg
     6001 cggcgggctg ggttatctgc tcggccgccg cagcctgcgc cgacgcaccc tcgccggtgc
     6061 ggcagagggc tgcgctgcgg acggcccgct tcacgacgac gccccggcga gcggcggcgc
     6121 ggtttcgagg cgggagtggg caggcacgcc tgcagacgcc gacgattgcg cgccggcgag
     6181 cgcgctcccc catgccctcc tccccgccgc gcacgccgct ccgcccgcgg gcgcaggcgc
     6241 ggcgatcggg gtgatcgcga ccgaatccta tcgcgaggtg gtcgattcgc tcagcgaggt
     6301 gctcttccgc accgacgagc ttggccgcct ggtttttctc aacgacgcct ggaaggatct
     6361 ctccggtttc gatctggacg ccacgcgcca gcatgcgctg accgatttcc tccatccaga
     6421 cgaccgcttg cgcgcacgcg acgcaatccg cgccctgctg tgcggagacg gccaggaatg
     6481 gaccgacgag ttgcggctgc gcacgctctc cggcgagatc cgctgggtgg cgatcgactg
     6541 ccgtgccttg cgcgacggca gcgggcagga aagcgggatc gccggcacca tcgacgacat
     6601 ctcggcgcgc aagatcgccg agttcagcct gcgcaacctg aaccaggagc tcgagtcgcg
     6661 ggtgcgctcg cgcaccgccg aactcgagac cgcggtgcgc gagctcgagg ccttctcgta
     6721 ttccgtctcg cacgacctgc gtgcgccgct gcgcgccatc gacggcttcg cgcgcatcct
     6781 cgtcgaggag gccgggccgc ggctcgacga acgccaacgc gagcagctcg tgcgcatccg
     6841 cgccggcgcc gagcgcatgg cgatcctgat cgatgcgctg atcgacctcg ccagcgtctc
     6901 ccggcaaccg ttgcgcagga ggccggtcga cctgtcgcgc attgccgaag cggtgatccg
     6961 cgacctgcag gcggagtcgc ccgggcgcgt ggtcgcggtc gagatcacca gcgacatgac
     7021 ggtggtcgcc gacccggtgc tgatgcacgt gctgctcgac aacctgctgc gcaatgcatg
     7081 gaagttcaca agccagtgcg aacatccgcg tatcgtcttc ggcgcggaac gcgacggcga
     7141 gcgcacggtg ttccacgtcg aggacaacgg cgccggattc gagatgaact acgcgggcaa
     7201 gctcttccag cccttccagc gcctgcacgc gcagcacgag tttcccggca ccgggatcgg
     7261 gctcgccacc gtacaacgca tcgtcgcccg ccatgagggc cgggtgtggg ccagcgcgga
     7321 accgggcaag ggcgcgcgct tctgcttcat gctggggcat tgagggtgac gacgccgcgc
     7381 tcggaaacgg gaaagcccac tccggacagc ggcggtcggt cggccgcggc acgaatcgca
     7441 ggcggcagag acaaaggtct caactgcccg ttgtgcactg cagtacaatg aattccattc
     7501 cgcgcgcgcc ctgcgcccgc caaaccattg cagcacacca ctcggacctc aggtggccac
     7561 catgaaagaa aagcactatc tgaccccgct cctcgagccg cgctccgtcg gcatcatcgg
     7621 cgccagcgaa cgcgaggcct ccctcggcag cgtgctcatg cgcaacatgc tcgaggccgg
     7681 ctacaagggc aagctgttcg cgatcaaccc gaagcacgag aaggtgcacg gggtcgcctg
     7741 ctacaagtcg gtggaagacg tgccgcagcg cctggacctg gtggtgatgg caatccgcgc
     7801 cgagaagacc ccggcgctga tggaggcctg cggccgcgcc ggggtgaagg cggtgatcct
     7861 gctgtcgggc ggcttctccg aatcgggcgc gcgcggcgcg ctgctcgagc gccaggtggt
     7921 cgaagccgcg caccgccacc gcatccgcct gctcggcccc aattgtctgg gcgtcatgcg
     7981 gccgcagctc ggcgtcaacg ccaccttcgc gcacgccagc gcactcaagg gcagcatcgg
     8041 cctgatctcg cagtcgggcg cgctgtgcgc ggccatcctc gactgggcca aaccgaacaa
     8101 cgtcggcttc tcgaccgtgg tgtccctggg ctcctcgtcc gacatcgact tcggcgaggt
     8161 gctcgaatac atgatctcgg acccgcgcac cgagagcatc ttcctctacg tcgaaggcat
     8221 ccgcgacgcc cgccgcttca tgagcgcgct gcgcggcgcc gcgcgcgtca agccggtgct
     8281 gctggtcaag gccggccgcc accccggcgc ctcgcgcgcg atcctgtcgc acagcggcgc
     8341 gccgatgggc gaggacgcgg tgttcgacgc cgcgctgcgt cgcgccggcg tgatccgcct
     8401 gtacaacatg ggccagctct tcgccgcggc caacgcgctg ttctcgcact tccgcccgcg
     8461 cggcaaccgc ctcgcgatca tcaccaacgg cggcggcccg ggcgtcatgg cggccgaccg
     8521 cgcggccgac atcggcat
//
LOCUS       DAOQBS010000129         8497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_7863_length_8497_cov_16.773632, whole genome shotgun sequence.
ACCESSION   DAOQBS010000129 DAOQBS010000000
VERSION     DAOQBS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7863_length_8497_cov_16.773632"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PKN25_12565"
     CDS             <1..981
                     /locus_tag="PKN25_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV91777.1"
                     /translation="RNSEVIHAGLYYPPGSLKARLCVEGRQRLYAWCAQHGVSHARCG
                     KLVVASAAAQAPALQAIAERARANGVDDLRRLDAAELAALEPALAGHAALLSPSTGIV
                     DSHGLMRSLLGEAERHGAMLALGSAVLEGRAEADGIVLRIGGETGTEVKAKRVVNAAG
                     LDAPALGRAIRAPASVQAPRAHYARGVYFSYAGKAPFSRLIYPIPEPGGLGVHLTLDL
                     GGQAKFGPDVEWIAAPDYTVDPTRVERFAGAIRRWWPDLDAQRLQPGYAGVRPKIVGP
                     GEPDADFRIDGPSAHGTPGLVHLYGIESPGLTAALAIGEHVAALLAAEGR"
     gene            complement(1019..2320)
                     /gene="gabT"
                     /locus_tag="PKN25_12570"
     CDS             complement(1019..2320)
                     /gene="gabT"
                     /locus_tag="PKN25_12570"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HNV91778.1"
                     /translation="MNANTIPPGLHNADFMARRAAALPRGVGQAHPLFATKALNAEVW
                     DVEGRRFIDFVAGIAVVNTGHCHPRVVAAAQAQLTHFSHTCFQVVAYDGYLRLAERLN
                     ALAPGDAAKKTMLVSTGAEAVENAVKIARAFTGRPGVIAFNGAFHGRTLLALGLTGKV
                     DPYKLGVGPFPGEIYHAPYPCALHGISVEDAIHGIELLFKNDIEARRVAAFIVEPVQG
                     EGGYYPAPAEFLQRLRALADKHGILLIADEIQSGIGRCGKMFAVEHSGVVPDIITLAK
                     GLGGGFPVAAVVGRAEVMDALAPGGLGSTYAGAPMACAAALAVLDVMEEEKLCERALA
                     IGEHMQRRLRELAARHKCIGDVRGLGAMVAVEFFHDGDHARPAPEIAKAVIAAALQRG
                     LLLLSCGSYGNVLRLMVPLTIEQAVLDEGLDLLAAAMDAAA"
     gene            complement(2359..3819)
                     /gene="gabD"
                     /locus_tag="PKN25_12575"
     CDS             complement(2359..3819)
                     /gene="gabD"
                     /locus_tag="PKN25_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNV91779.1"
                     /translation="MSLNLKDPDLFRTRCHVDGQWIDADDGATTSIRNPATGEVLGTI
                     PRMGAAETRRAIAAANAAWPAWRALTAGARAKILRRWFELILANQEDLAVLMTSEQGK
                     PLAEARGEVLYAASFIEWFAEEGKRIYGDVIPGHQPDKRIVVTKEPIGVCAAITPWNF
                     PAAMITRKAGPALAAGCTMVLKPATQTPYSALALAVLAERAGVPKGVFSVVTGGAAEI
                     GGELTANPIVRKLTFTGSTEIGVKLMAQCAPSVKKLSLELGGNAPFIVFDDADLDAAV
                     EGAIASKYRNTGQTCVCANRLLVQDGVYDAFAARLAAAVARLKVGNGLAEGSTQGPLI
                     DMNAVAKVEEHIADAVEKGARVLAGGKRHALGGSFFEPTILADVTPAMKVAREETFGP
                     VAPLFRFKDEAEAIRMANDTEFGLAAYFFANSMNRVWRVAEALEYGIVGINTGIISTE
                     VAPFGGMKSSGLGREGSKYGIEDYLEVKYLCMGGVQ"
     gene            complement(3896..4720)
                     /locus_tag="PKN25_12580"
     CDS             complement(3896..4720)
                     /locus_tag="PKN25_12580"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV91780.1"
                     /translation="MLQPYASPVERAWYYGMRGLVGLILAFLFLPILVIVPLSFNADS
                     FLMYPMSGFSLRWYEEFATSPAWRQALMNSLIVSPLATLLAMVLGTLASIGLVRADFP
                     GKALLTAVLISPMVVPVVVIGVAMYIFFAPLGLTGSYTGLVLAHAILGVPFVVTTVTA
                     TLQGFDFNLVRAASSLGANPLTTFFRVTLPMVAPGIISGGLFAFATSFDEVVVTLFLA
                     SPEQATLPRQMFAGIRENISPTIAAVATILIVLSTLMLMTLEWLRRRAERMRGARR"
     gene            complement(4732..6006)
                     /locus_tag="PKN25_12585"
     CDS             complement(4732..6006)
                     /locus_tag="PKN25_12585"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV91781.1"
                     /translation="MSTAVDTSAAVPLPAEGNGTPLKKRLRRAERRRNLLFGALILPL
                     ALVVLVAFIWPIGSLLTIGVDNPEIHDNLPRTLAALEDWDGRAPPAEAAFRALADELA
                     AAKGSPAIAAIAKRLNREDAGYRSLVMKTARKLPLDPAVPAREALLAIDAQWGELATW
                     QAIHRAGGALTPFYLLAAIDRTIDADGNIVAAPADERIYVDVLLRTFWMSLVVTVCCL
                     VLGFPVAYLMANLSTRHSNMLMVFVLLPFWTSVLVRVAAWIVLLQNEGLVNRLLMWLG
                     LTDSPLQLLFNRFGVYVAMVHILLPFMVLPLYSVMKGISPVYVRAALSLGCPPFRSFW
                     KIYFPQTLPGIGAGGLLVFIMCMGYYITPALLGGPKEQMLSYFIAFYTNQTINWGMAA
                     ALSAVLLAATLLLYMVYARYLGPTRVKYMKAR"
     gene            complement(6124..7164)
                     /locus_tag="PKN25_12590"
     CDS             complement(6124..7164)
                     /locus_tag="PKN25_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV91782.1"
                     /translation="MKHAASFLRAAVLVALGGAVGTASAEITVISFGGASQKAQDKAY
                     YAPFTKATGVKVVAGEYNGEQAKVKAMVEAGNVTWDVLEVESPELVRGCEEGLFEKID
                     FAQVGDKADFVPAAVSECGVGIFVWSTALAYNADRLKEAPTSWADFWNVDKFPGKRGL
                     RKGAKYTLEFALLADGVPTSEVYKVLATPAGVDRAFAKLDKLKANIQWWEAGAQPPQL
                     LASGDLVMSAAYNGRISAAQVEGKNLKVVWNGSIYDVDSWTIPKGSPKKAEALKFIRF
                     ASQPENQAVFSGEIAYGPVNLKAVPAIDAKVAAGLPTAPANMKGAMATDTEFWVEHGE
                     NLEQRFNAWAAR"
     gene            complement(7252..8358)
                     /locus_tag="PKN25_12595"
     CDS             complement(7252..8358)
                     /locus_tag="PKN25_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV91783.1"
                     /translation="MEAVHNSQAAHVSFRNVQKTYDGETLIVRDLNLDIRRGEFLTLL
                     GPSGSGKTTCLMMLAGFETPTGGEIRLDGEVINRVPPYRRNIGMVFQNYALFPHMTVA
                     DNLRFPLRVRKLAANEVEARVKRALGMVQLEAFGNRYPQQLSGGQQQRIALARALVFD
                     PQLVLMDEPLGALDKNLRERMQLEIKHIQQNLGITVVFVTHDQSEALTMSDRIAVFNQ
                     GVIQQIDAPETLYEYPANSFVADFIGENNALDGTVVAVAGEECDVRLDCGTVLRARTG
                     NVGAGGRTRIAVRPERVRLAGEEGGTNRLDATVRELIYLGDQIRLRIELAGKHEFTIK
                     APVSQLDRSLRPGDAIRVGLDPAHTRALDVPAAH"
BASE COUNT         1274 a         2831 c         3115 g         1277 t
ORIGIN      
        1 cgcaacagcg aggtgatcca cgccgggctg tactacccgc ccggctcgct caaggcccgc
       61 ctgtgcgtgg agggccgcca gcgcctttac gcctggtgcg cgcagcacgg cgtcagccac
      121 gcccgctgcg gcaagctggt ggtcgccagc gccgcggcgc aggcgccggc gctgcaggcg
      181 atcgccgagc gcgcgagagc caacggcgtg gacgacctgc gccggctcga cgccgccgag
      241 ctcgccgcgc tggagccggc gctcgccggc cacgctgcgc tgctgtcgcc ctcgaccggc
      301 atcgtcgaca gccacggcct gatgcggtcc ctgctcggcg aggccgagcg ccatggcgcg
      361 atgctcgcgc tcggcagcgc ggtgctggag ggccgtgcgg aggccgacgg catcgtgctg
      421 cgcatcggcg gcgagaccgg aaccgaggtg aaggcgaaac gggtggtcaa tgccgcgggc
      481 ctggatgcgc ccgcgctcgg gcgcgcgatc cgggcgcccg cttccgtcca ggccccacgg
      541 gcgcattacg cgcgcggggt gtatttcagc tacgcgggca aggcgccgtt ctcgcgcctg
      601 atctacccga tcccggagcc gggcgggctg ggcgtgcacc tgaccctgga tctgggcgga
      661 caggcgaagt tcggtcccga cgtggagtgg atcgccgcgc cggactacac cgtggatcca
      721 acgcgcgtgg agcgcttcgc cggcgcgatc cggcgctggt ggccggacct ggacgcgcag
      781 cggctgcagc cgggctatgc cggcgtgcgc ccgaagatcg tcggccccgg cgagcccgac
      841 gccgacttcc gcatcgacgg cccgtcggcg catggcacgc cggggctggt gcatctctac
      901 ggcatcgaat cgcccggcct caccgcggcg ctcgcgatcg gcgagcacgt cgcggcgctg
      961 ctcgcggccg aggggcgctg actccgcagc gctccccggc gcacaggtcc gaccccgatc
     1021 aggccgcggc gtccatcgcc gcggcgagga ggtcgaggcc ttcgtcgagc accgcctgct
     1081 cgatggtgag cggcaccatc aggcgcagca cgttgccgta gctgccgcag gacagcagca
     1141 acaggccgcg ctgcagcgcc gcggcgatca ccgccttggc gatctccggc gccggacgcg
     1201 catggtcgcc gtcgtggaag aactccaccg ccaccatcgc acccaggccg cgcacgtcgc
     1261 cgatgcactt gtggcgcgcg gccagctcgc gcaggcgacg ctgcatgtgc tcgccgatcg
     1321 cgagcgcgcg ctcgcacagc ttctcctctt ccatcacgtc gagcacggcg agcgcggcgg
     1381 cgcaggccat cggcgcgccg gcataggtgc tgccgaggcc gcccggggcg agcgcgtcca
     1441 tgacctcggc acggcccacc accgccgcga ccgggaagcc gccgccgagg cccttggcca
     1501 gggtgatgat gtcgggcacc acgccgctgt gttcgaccgc gaacatcttg ccgcagcggc
     1561 cgatgccgga ctggatctcg tcggcgatca gcaggatgcc gtgcttgtcg gcgagcgcgc
     1621 gcaggcgctg caggaactcg gcgggcgcgg ggtagtagcc accctcgccc tgcaccggct
     1681 cgacgatgaa ggcggcgacg cgacgcgcct cgatgtcgtt cttgaacagc agctcgatgc
     1741 cgtggatggc gtcctccacg ctgatgccgt gcaacgcgca gggatagggg gcatggtaga
     1801 tctcgcccgg gaaggggccg acgccgagct tgtaggggtc gaccttgccg gtgaggccga
     1861 gcgcgagcag cgtgcggccg tggaacgcgc cgttgaaggc gatcacgccc gggcggccgg
     1921 tgaaggcgcg cgcgatcttg accgcgttct cgaccgcctc ggcgccggtg ctcaccagca
     1981 tggtcttctt cgccgcatcg cccggggcga gcgcgttgag gcgctcggcc aggcgcaggt
     2041 agccgtcgta ggccacgacc tggaagcagg tgtggctgaa gtgggtgagc tgcgcctgcg
     2101 ccgcggcgac cacgcgcggg tggcagtgac cggtgttgac cacggcgatg ccggcgacga
     2161 aatcgatgaa gcggcggcct tcgacgtccc acacctcggc attgagggcc ttggtggcga
     2221 acagcgggtg ggcctggccc acgccgcgcg gcagcgcggc ggcgcgacgg gccatgaagt
     2281 cggcgttgtg caggccgggg gggatggtgt tggcgttcat gggtgtctcc tgtcgattcg
     2341 cttggggggg acggcggctc actgcacgcc gcccatgcac agatacttga cctcgaggta
     2401 gtcctcgatg ccgtacttgg aaccttcgcg gccgaggccg gaggacttca tgccgccgaa
     2461 gggcgcgacc tcggtcgaga tgattccggt gttgatgccg acgatgccgt actcgagcgc
     2521 ctcggcgacg cgccacacgc ggttcatcga gttggcgaag aaataggcgg cgaggccgaa
     2581 ctcggtgtcg ttggccatgc ggatcgcctc ggcctcgtcc ttgaagcgga acagcggcgc
     2641 caccgggccg aaggtctcct cgcgcgccac cttcatcgcc ggggtcacgt cggccaggat
     2701 ggtgggctcg aagaagctgc cgccgagcgc gtggcgcttg ccgccggcga gcacgcgcgc
     2761 gcccttctcc accgcgtcgg cgatgtgctc ctcgaccttg gccaccgcgt tcatgtcgat
     2821 cagcgggccc tgggtgctgc cctcggcgag gccgttgccc accttcaggc gcgccaccgc
     2881 ggcggcgagc ctggcggcga aggcgtcgta cacaccgtcc tgcaccagca ggcggttggc
     2941 gcacacgcag gtctggccgg tgttgcggta tttggaagcg atcgcgccct cgaccgcggc
     3001 gtcgaggtcg gcatcgtcga agacgatgaa gggcgcattg ccgccgagct cgagcgagag
     3061 cttcttgacg ctcggcgcgc actgcgccat cagcttgacg ccgatctcgg tggagccggt
     3121 gaaggtgagc ttgcggacga tcgggttggc ggtcagctcg ccgccgatct cggccgcgcc
     3181 gccggtgacc acgctgaaca cgcccttcgg cacccccgcg cgctcggcga gcaccgcgag
     3241 cgcgagcgcc gagtaagggg tctgggtggc gggcttgagc accatcgtgc atccggccgc
     3301 gagcgccgga ccggccttgc gcgtgatcat cgccgcgggg aagttccacg gcgtgatcgc
     3361 cgcgcacacc ccgatcggct ccttggtgac gacgatgcgc ttgtcgggct ggtggccggg
     3421 gatcacgtcg ccgtagatgc gcttgccttc ctcggcgaac cactcgatga aggaggcggc
     3481 gtagagcacc tcgccgcgcg cctcggcgag cggcttgccc tgctcgctgg tcatcagcac
     3541 ggcgaggtct tcctggttgg cgaggatgag ctcgaaccag cgccgcagga tcttcgcgcg
     3601 cgcgccggcg gtcagcgcac gccacgccgg ccatgcggcg ttggcggccg cgatggcgcg
     3661 ccgggtctcg gccgcgccca tgcgcgggat ggtgccgagc acctcgcccg tggccgggtt
     3721 gcggatgctc gtggtggcgc cgtcgtcggc gtcgatccac tgcccatcga catggcagcg
     3781 ggtgcggaag aggtcgggat ctttcaggtt caggctcatg gtcggggtcc gtcgggtggt
     3841 cgggggaaag cttgggttct tggagtcggt gccgggggtg gcgcgagcgc ggcgctcagc
     3901 ggcgcgcgcc acgcatgcgc tcggcgcgcc ggcgcaacca ttcgagcgtc atcagcatca
     3961 gcgtcgacag cacgatcagg atggtcgcga ctgccgcgat ggtcgggctg atgttctcgc
     4021 ggatgccggc gaacatctgc cgcggcagcg tcgcctgctc ggggctggcg aggaagagcg
     4081 tgaccacgac ctcgtcgaag gaggtggcga aggcgaacag gccgcccgag atgatcccgg
     4141 gcgcgaccat cggcagggtg acgcggaaga aggtggtgag cgggttggcg ccaaggctgg
     4201 aggccgcgcg caccaggttg aagtcgaagc cctgcagcgt cgcggtcacc gtggtgacga
     4261 cgaagggcac gccgaggatg gcatgcgcca gcaccaggcc ggtgtagctg ccggtgaggc
     4321 cgagcggggc gaagaagatg tacatcgcca cgccgatcac caccaccggc accaccatcg
     4381 gcgagatcag caccgcggtg agcagcgcct tgccgggaaa atcggcgcgc accaggccga
     4441 tcgaggccag cgtgccgagc accatcgcca gcagggtggc gagcggcgac acgatcaggc
     4501 tgttcatcag cgcctggcgc caggccggcg aggtggcgaa ctcctcgtac cagcgcagcg
     4561 agaagcccga catcggatac atcaggaagc tgtcggcgtt gaaggacagc ggcacgatga
     4621 ccaggatggg caggaacagg aaggccagga tcagcccgac cagcccgcgc atgccgtaat
     4681 accatgcccg ttccacgggc gaagcgtagg gttgcagcat cgtcgtcgtc ctcagcgcgc
     4741 cttcatgtac ttgacccggg tcggcccgag atagcgggca tagaccatgt acagcagcag
     4801 ggtcgccgcc agcagcaccg cggaaagcgc ggcggccatg ccccagttga tcgtctggtt
     4861 ggtgtagaag gcgatgaagt agctcagcat ctgctccttc ggaccgccga gcagcgcggg
     4921 cgtgatgtag tagcccatgc acatgatgaa cacgagcagg ccgccggcgc cgatgccggg
     4981 cagggtctgc gggaagtaga tcttccagaa gctgcggaag ggcgggcagc cgagcgagag
     5041 cgcggcgcgc acatacaccg gcgagatgcc cttcatcacc gagtacagcg gcagcaccat
     5101 gaagggcagc aggatgtgga ccatcgccac atacacgccg aagcggttga agaggagctg
     5161 gagcgggctg tcggtgaggc cgagccacat cagcaggcgg ttcaccaggc cctcgttctg
     5221 cagcagcacg atccacgccg ccacccgcac cagcaccgag gtccagaacg gcagcagcac
     5281 gaagaccatc agcatgttgc tgtggcgggt ggacagattc gccatcaggt aggccaccgg
     5341 gaagccgagc accaggcagc acacggtcac gaccaggctc atccagaagg tgcgcagcag
     5401 cacgtccaca tagatgcgct cgtccgcggg ggcggcgacg atgttgccgt cggcgtcgat
     5461 cgtgcggtcg atcgcggcga gcaggtagaa cggcgtcagc gcgccgccgg cgcggtggat
     5521 cgcctgccag gtggcgagct cgccccactg cgcgtcgatc gccagcagcg cctcgcgcgc
     5581 cggcacggca ggatcgagcg gcagcttgcg cgcggtcttc atgaccaggc tgcggtagcc
     5641 ggcgtcctcg cggttcaggc gcttggcgat cgcggcgatc gccggcgagc ccttggccgc
     5701 ggcgagctcg tcggcgagcg cgcggaaggc ggcctcggcc ggtggcgcgc gaccgtccca
     5761 gtcctcgagc gcggccagcg tgcgcggcag gttgtcgtgg atctcggggt tatcgacacc
     5821 gatcgtcagc agcgagccga tcggccagat gaaggccacc agcaccacca gcgcgagcgg
     5881 caggatcagg gcgccgaaga gcaggttgcg gcggcgctcg gcgcggcgca ggcgcttctt
     5941 cagcggcgtg ccattgccct cggcgggcaa gggaacggca gcggaggtgt cgacggcagt
     6001 gctcatggac gagtccgttc gggcagatgg agacggtggc cgcgcgttgc ggccgccagc
     6061 gcgggccgga cgcccgcggc ggcacgggca tgcccgcgca cgggcatgcg acgaaggcag
     6121 cgctcagcgc gcggcccagg cgttgaagcg ctgctcgagg ttctcgccgt gctcgaccca
     6181 gaactcggta tcggtcgcca tcgcgccctt catgttcgcg ggcgcagtcg gcaggcccgc
     6241 ggcgaccttg gcgtcgatcg ccggcaccgc cttcaggttg accgggccgt aggcgatctc
     6301 gcccgagaac accgcctggt tctccggctg gctggcgaag cggatgaact tgagcgcctc
     6361 cgccttcttc ggcgagccct tggggatggt ccacgaatcg acgtcgtaga tgctgccgtt
     6421 ccacaccacc ttgaggttct tgccttcgac ctgcgcggcg gagatgcggc cgttgtaggc
     6481 ggcgctcatg accaggtcgc ccgaggcgag cagctgcggc ggctgcgcgc cggcctccca
     6541 ccactggatg ttggccttga gcttgtcgag cttggcgaag gcgcggtcga caccggccgg
     6601 ggtggcgagc accttgtaga cctcgctcgt cggcacgcca tcggcgagca gggcgaactc
     6661 gagggtgtac ttcgcgccct tgcgcagccc gcgcttgccg gggaatttgt ccacgttcca
     6721 gaagtccgcc caggacgtcg gcgcctcctt gaggcggtcg gcgttgtagg cgagcgcggt
     6781 cgaccacacg aagatgccga caccgcattc gctcacggcg gcagggacga aatcggcctt
     6841 gtcgcccacc tgcgcgaagt cgatcttctc gaacaggcct tcctcgcagc cgcgcaccag
     6901 ctcgggcgac tcgacctcga gcacgtccca ggtcacgttg ccggcctcga ccatggcctt
     6961 caccttggcc tgctcgccgt tgtactcgcc ggcgacgacc ttgaccccgg tggccttggt
     7021 gaagggcgcg tagtaggcct tgtcctgcgc cttctgcgag gcgccgccga aggagatgac
     7081 ggtgatttcg gccgatgcgg tgccgacggc gcccccgagg gcgacgagga cggcggcgcg
     7141 aaggaaggat gcggcgtgct tcatgatgtg tctccggttc tggttatgtg ggtgctgcgg
     7201 gtgaagaccg gcggccggcc gggactgcgg atgcggcggt gggcggatgc ctcagtgcgc
     7261 cgcgggcacg tcgagcgccc gcgtgtgcgc cggatcgagc ccgacgcgga tcgcgtcgcc
     7321 ggggcgcagc gagcggtcga gctgcgagac cggcgccttg atggtgaatt cgtgcttgcc
     7381 ggcgagttcg atgcgcaggc ggatctggtc gccgagatag atcagctcgc gcacggtggc
     7441 gtcgagccgg ttggtgccgc cctcctcccc ggcgagccgc acgcgctccg ggcgcaccgc
     7501 gatgcgggtg cggccgccgg cgccgacgtt gccggtgcgc gcgcgcagca ccgtgccgca
     7561 gtccaggcgc acgtcgcact cctcgcccgc caccgcgacc acggtgccgt cgagcgcgtt
     7621 gttctcgccg atgaagtcgg cgacgaagga gttcgcgggg tattcgtaca gggtctcggg
     7681 ggcgtcgatc tgctggatga ccccctgatt gaacaccgcg atgcggtccg acatggtcag
     7741 cgcctcgctc tggtcgtggg tgacgaacac caccgtgatg ccgaggttct gctggatgtg
     7801 cttgatctcg agctgcatgc gctcgcgcag gttcttgtcg agcgcgccga gcggctcgtc
     7861 catcagcacc agctggggat cgaacacgag cgcgcgggcg agcgcgatgc gctgctgctg
     7921 gccgccggag agctgctgcg ggtagcggtt gccgaaggcc tcgagctgca ccatgccgag
     7981 cgcgcgcttc acccgcgcct cgacctcgtt cgccgccagc ttgcgcacgc gcagcgggaa
     8041 gcgcaggttg tcggccaccg tcatgtgcgg aaacagcgca tagttctgga acaccatgcc
     8101 gatgttgcgg cggtagggcg gcacccggtt gatgacctcg ccgtcgaggc ggatctcgcc
     8161 gccggtgggc gtctcgaagc cggcgagcat catcaggcag gtggtcttgc ccgaaccgga
     8221 cggcccgagc agggtgagga actcgccgcg gcgaatgtcg agattcaggt cccgcacgat
     8281 gagggtttcg ccgtcgtagg tcttctggac gttgcgaaag ctgacgtggg ctgcctggct
     8341 gttgtggacc gcttccatct gtctccgtct cctgcaaggg gcgctgtgcg ctctggcgat
     8401 cgaatcctag gccacgaatt ggcccaggct tgatccaatt acttataatt tgatagaacc
     8461 aatttttgct gcactgcaat ggattgccga ctcagct
//
LOCUS       DAOQBS010000130         8423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_7974_length_8423_cov_17.078155, whole genome shotgun sequence.
ACCESSION   DAOQBS010000130 DAOQBS010000000
VERSION     DAOQBS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7974_length_8423_cov_17.078155"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..591)
                     /gene="rlmH"
                     /locus_tag="PKN25_12600"
     CDS             complement(121..591)
                     /gene="rlmH"
                     /locus_tag="PKN25_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNV91784.1"
                     /translation="MKLIIVAVGHRMPAWVQAGFDEFARRMPRELQLQLVEVKAEPRS
                     GGKTVEAMMAAEAARIEATLPPRCRRVILDERGADLSTLALARRLESWQGAGDDVALI
                     VGGPDGLAPAFKAGAHEAIRLSSLTLPHALVRPLLAEALYRAWTVLKNHPYHRE"
     gene            complement(689..1036)
                     /gene="rsfS"
                     /locus_tag="PKN25_12605"
     CDS             complement(689..1036)
                     /gene="rsfS"
                     /locus_tag="PKN25_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNV91785.1"
                     /translation="MDTPTLEKIVVAALEDIKARDIEVIDTSKHTPLFDRIVVASAES
                     GRQTRALAQNVHDKVKEAGGEIIGTEGQETGEWVLVDLGNIVVHVMQPAVRAHYKLEE
                     LWNTPPSSRRNAA"
     gene            complement(1067..1777)
                     /gene="nadD"
                     /locus_tag="PKN25_12610"
     CDS             complement(1067..1777)
                     /gene="nadD"
                     /locus_tag="PKN25_12610"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNV91786.1"
                     /translation="MATEPAPSPARAAFEPPARAPLGVLGGTFDPIHTGHLRLAEEAR
                     EALGLDGVRFIPAGQPPHRGEPGSTAEDRLAMARLATAGNPGFSVDDGEVRAQQKSYT
                     VPTLERLRAEIGPQQPLVLILGADAFAGLPTWHRWTDLFALAHIAVANRPGYAPHGRR
                     WPATLSPALDAACAGRHTADPADLRRAPAGRVVPFDMTPLAISASLIRDLVRDGHSAR
                     YLLPESVLDYIAAHHLYR"
     gene            complement(1767..2570)
                     /locus_tag="PKN25_12615"
     CDS             complement(1767..2570)
                     /locus_tag="PKN25_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HNV91787.1"
                     /translation="MQARQLPMPRGWAWLREGLQLWRRNPALLTFATFGYLLSIIVIS
                     LVPLLGQLVASLLMPALSVGVLNACRAIDGGRKTGPDVLFSGFKSNLQGLVVVGGLYL
                     AATLVVLMLVMLVDDGSLAAVMRGEDMDAQAAADSSLGFTLVVGLVLSTPVMMAYWFA
                     PALAGWWKLPAPKAMFFSFYACLRNWRPFVAYALGLAIFAGLLPGLVVAVLGLLSPTL
                     GSVAALPVPLIILPIVFASFYTATRDVFGLPESNPARDPAALDDARDGD"
     gene            complement(2584..3591)
                     /locus_tag="PKN25_12620"
     CDS             complement(2584..3591)
                     /locus_tag="PKN25_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HNV91788.1"
                     /translation="MNATLPPPRRPHPPPPAPGDVVPGHALDWLAAGWRIFMRAPLVW
                     ALQALIFFVILAALGMVPFLGWAAAPVTLPVLVAGMLSGARSLDRGDRLHVGQLFDGL
                     RQHAGNLLLVGLFHLLGLLLAALAAAAIGGSAMLTGAAAGALGAIAGAGVAAGGMMLG
                     VVVFTVLWALLMMALWFAPALVMLHEVAPLDAMRLSARACFHNLLAFVVLAALLYVLV
                     WVAMIPAGLGMLVLVPMIAGALYAAWKETFSPLLPAPAAPPAPAAGATSGESVPPAPV
                     LLAPAADPAPDDAATSAAPPAPEPGPHPDLAPGSTPAPAPGPESDPAPDSAPADTPPK
                     P"
     gene            complement(3642..4598)
                     /locus_tag="PKN25_12625"
     CDS             complement(3642..4598)
                     /locus_tag="PKN25_12625"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNV91789.1"
                     /translation="MSVFTPVPESVLADWLKDYAIGRLVELEGISAGVQNSNFFVTTT
                     LGRYVLTLFEGIPRAELPYYLHLMAHLSRHGLPVPGPIANRHNEYLGTLQERPAALVV
                     RLSGRSEMSPGLAHCGRVGAMLAGLHLAGQSYGRRQENPRGAAWRAATAQVLRPFLPA
                     DEQTLLDAEIAFQATIDVAALPAGAIHADLFRDNVLWDTDADGGVRIGGVIDFYFAGY
                     DALLFDVAVTVNDWCSTPDGGLDAERAAALLDAYHAERPFTDAERAAWPAMLRAAALR
                     FWMSRAADFHLPRAGEMVLVKDPNEYRDILRLRIAAAPPLPR"
     gene            complement(4629..5195)
                     /locus_tag="PKN25_12630"
     CDS             complement(4629..5195)
                     /locus_tag="PKN25_12630"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNV91790.1"
                     /translation="MSTTAAPLVRSFAPLFRADARVLVLGSMPGTASLAAAQYYAHPR
                     NAFWPIMGALFGAGAELPYAQRLERLAAAGVALWDVIGRCERAGSLDSAIAPDSIEAN
                     DFAGLFAACPDIGHVFFNGTAAETAFRRHVRGRIALPPLHFTRLPSTSPAHAARGLEA
                     KLAAWQVVKAAAHRAAAARIDIPAAQAA"
     gene            complement(5192..5512)
                     /locus_tag="PKN25_12635"
     CDS             complement(5192..5512)
                     /locus_tag="PKN25_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HNV91791.1"
                     /translation="MRRTLIVLWLAAAVPAFAQQPANLEPIPEPPPMPAEGAADEPEV
                     TIVKRGEDTVTEYRLRGKLYMVKVTPPHGVPYYLIDKEGNGQMVRHDGTPELAVPMWV
                     IKSW"
     gene            complement(5558..6283)
                     /locus_tag="PKN25_12640"
     CDS             complement(5558..6283)
                     /locus_tag="PKN25_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNV91792.1"
                     /translation="MTAKDKLPRSVPDSTAPRFNARESWRIFGIMAEFVEATERLAAI
                     RPAVSIFGSARTPPDHKYYMLTEKIARLLSDSGFAVISGGGPGIMEAANKGAFFGKSP
                     SVGLNIQLPLEQAANPYQDISQTFQHFFARKFMFVKFATAYVVMPGGFGTLDELLEAM
                     TLIQTRKSRKIPIILVHRPFWQGLLDWLRERLVAEGMINAEDLDLVQVIDEPEEVVEA
                     IFKHYERRGFQPLPAEREMMLNL"
     gene            6307..>8423
                     /gene="polA"
                     /locus_tag="PKN25_12645"
     CDS             6307..>8423
                     /gene="polA"
                     /locus_tag="PKN25_12645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNV91793.1"
                     /translation="MPTLLLVDGSSYLYRAFHALPDLRNSQGEPTGAIRGVLSMLRRL
                     ESDYKAEFRACVFDAKGKTFRDDWYPEYKSHRPPMPDDLRAQIAPLHEAVRAEGWPLL
                     SVEGVEADDVIGTLTRLALERGWEVVISTGDKDLTQLVRPGVRWVNTMSEEVLDEAGV
                     AAKFGVPPERIVDYLALVGDTVDNVPGVEKCGPKTAVKWLTEYGTLDNLVANADKVGG
                     KVGENLRRHLDFLPLGRKLVTVATDVELPVGLDELPARADDKAALRALYERFEFKSWL
                     KDLDGGAESVAASKAASDARRFDRQENRGQTPISEAATTTPGEGGVCAISADEQMKDL
                     TPVYLSILDWPTFDAWLAKISAAELTAFDTETTSLDPMAARLVGMSFAVEPGEAAYLP
                     LAHRGAAQLPEAPEQLPLDEVLDRLRPWFESDRHAKLGQNLKYDAHVLANHGIALRGI
                     AHDTLLESYVLESDKAHDMDSLAKRHLGLATIPYTEVCGKGAKQIGFDEVDVARATEY
                     AAEDADVTLRLHQHLCPQLQALPQLEALYRELEMPVLGVLQRMERTGVLIDPFLLGQH
                     SEELGRRLYTLEGEAHALAGQPFNLGSPKQLGEILFGKLGLPVVKKTATGQPSTDEEV
                     LEKLAEDYPLPKLLLEHRGLSKLKSTYADKLPRMVNPRTGRVHTSFSQATAVTGRLSS
                     SEPNLQNIPIRTPEGRRIRAAFIA"
BASE COUNT         1279 a         2764 c         3221 g         1159 t
ORIGIN      
        1 gcgccgccgt gccggcatca ccgccgcaat cgcgactcgc gtcgctccca cgggcgcctt
       61 cggagacgcg gtggttcgtg tgggagcgac gcaagtcgcg atcggccgat gctgcaggcg
      121 ctactcgcgg tggtaggggt ggttcttgag caccgtccac gcgcggtaga gggcttcggc
      181 cagcaggggg cgcaccaggg cgtgggggag ggtgaggctg gagaggcgga tggcctcgtg
      241 ggcgcccgcc ttgaacgctg gggcgaggcc gtcggggccg ccgacgatga gcgcgacgtc
      301 gtcgcctgcg ccctgccagg actccagccg gcgcgcgagc gccagcgtgc tgaggtcggc
      361 gccgcgctcg tcgaggatca cgcggcggca gcgcggcggc agggtggcct cgatgcgcgc
      421 ggcctcggcc gccatcatcg cctccaccgt cttgccgccg ctgcgcggct cggccttgac
      481 ctcgacgagc tgcaactgca gctcgcgcgg catgcggcgg gcgaactcgt cgaagccggc
      541 ctgcacccag gcgggcatgc ggtggccgac ggcgacgatg atcagtttca tggttgaacc
      601 tgcgcgcgtg cgtggacgag catgcgtcga aaaacgcgac cgccacccgc gccgtacgca
      661 ggcggcctct cgcgcggggc gctactcgtc aggcggcgtt gcgccggctg gacggcgggg
      721 tgttccagag ttcctcgagc ttgtagtgcg cgcgcaccgc gggctgcatc acgtgcacca
      781 cgatgttgcc gaggtcgacc agcacccact cgccggtctc ctggccttcg gtgccgatga
      841 tctcgccgcc ggcttccttg accttgtcgt gcacgttctg cgcgagcgcc cgggtctgac
      901 ggccggattc ggcgctggcg acgacgatgc ggtcgaagag gggggtgtgc ttcgaggtgt
      961 cgatgacttc gatgtcgcgt gccttgatgt cttcgagggc ggcgacgacg atcttttcga
     1021 gggtgggggt gtccattcgg atcctgtgtg ggccggcgcg gggcgctcag cggtagagat
     1081 ggtgcgcggc aatatagtcg agaaccgatt ccggcagcaa atagcgggca ctgtggccgt
     1141 cgcgcaccag atcgcggatc agcgaggcgg agatcgccag cggggtcatg tcgaagggga
     1201 cgacgcggcc cgccggtgcg cggcgcagat ccgcggggtc ggcggtgtgg cggcccgcgc
     1261 aggcggcgtc gagcgcgggc gacagggtgg cgggccagcg ccggccgtgg ggcgcgtagc
     1321 cggggcggtt ggcgaccgcg atgtgggcga gcgcgaacag atcggtccag cggtgccagg
     1381 tcggcagccc ggcgaaggcg tcggcgccga ggatcagcac cagcggctgc tgcgggccga
     1441 tctcggcgcg caggcgctcg agggtgggca cggtgtagct cttctgctgc gcgcgcacct
     1501 cgccgtcgtc cacgctgaag ccggggttgc cggcggtggc gagccgtgcc atggcgaggc
     1561 ggtcctcggc ggtgctgccg ggctcgccgc ggtgcggcgg ctggccggcg gggatgaagc
     1621 gcacgccgtc gaggccgagc gcctcgcgcg cttcttcggc gaggcgcagg tggccggtgt
     1681 ggatggggtc gaaggtgccg ccgagcacgc cgagcggggc ccgggcgggc ggctcgaaag
     1741 cggctctggc cggcgaaggc gcgggctcag tcgccatcgc gcgcgtcgtc gagtgcggcg
     1801 ggatcgcgcg ccgggttgct ctcggggagg ccgaacacgt cgcgggtggc ggtgtagaaa
     1861 ctcgcgaaca cgatcggcag gatgatcagc ggcaccggca gcgcggccac gctgcccagc
     1921 gtgggcgaga gcaggccgag caccgcgacc acgaggccgg gcagcaggcc ggcgaagatc
     1981 gccagcccga gcgcgtaggc gacgaagggc cgccagttgc gcaggcaggc gtagaagctg
     2041 aagaacatcg ccttgggtgc gggcagcttc caccagccgg cgagcgcggg cgcgaaccag
     2101 taggccatca tcaccggcgt gctcagcacc aggccgacca ccagggtgaa accgagcgag
     2161 gagtcggccg cggcctgcgc gtccatgtcc tcgccgcgca tcaccgcggc caggctgccg
     2221 tcgtcgacca gcatcaccag catcagcacc accagggtgg ccgcgaggta gagcccgccg
     2281 accaccacca ggccctgcag gttggacttg aagccggaga agagcacgtc gggaccggtc
     2341 ttgcgtccgc cgtcgatggc gcggcaggcg ttcagcacgc cgaccgagag cgcaggcatc
     2401 agcagcgagg cgacgagctg gccgagcagc ggcaccaggc tgatcacgat gatcgagagc
     2461 aggtagccga aggtggcgaa ggtgagcagc gccggattgc gccgccacag ctgcaggcct
     2521 tcgcgcagcc aggcccagcc gcgcggcatg gggagttgtc gtgcttgcat tcgtttccag
     2581 gggtcagggt ttcgggggcg tgtccgctgg cgcggagtcg ggagccggat cggattcggg
     2641 gccgggcgcg ggggcgggag tggagccggg cgcgaggtcc gggtgggggc ccggttcggg
     2701 agcgggcggt gccgcggagg tggctgcgtc atcgggcgcg gggtcggccg caggcgcgag
     2761 cagcacgggg gcgggcggga cgctttcgcc cgacgtcgcc cccgctgcgg gcgcgggcgg
     2821 ggccgcgggg gccggcagca gcggcgagaa ggtctccttc cacgccgcgt aaagcgcgcc
     2881 ggcgatcatc ggcaccagca ccagcatgcc caggccggcg gggatcatcg ccacccacac
     2941 cagcacgtag agcagcgcgg cgagcaccac gaaggcgagc aggttgtgga agcaggcgcg
     3001 cgcggacagc ctcatcgcat ccagcggcgc gacctcgtgc agcatcacca gcgccggggc
     3061 gaaccacagc gccatcatca gcagggccca caaaacggtg aacaccacca cgccgagcat
     3121 catgccgccg gcggcgacgc ccgccccggc gatcgcgccg agcgcgcccg ccgccgcgcc
     3181 ggtgagcatc gcgctaccgc cgatcgcggc cgcggccagc gccgcgagca gcaggccgag
     3241 gaggtggaag aggccgacca gcagcaggtt gccggcatgc tggcgcaggc cgtcgaagag
     3301 ctggccgacg tgcaggcggt ctccgcggtc gagtgagcgc gcaccggaga gcatgcctgc
     3361 gaccagcacc ggcagggtca cgggtgcggc cgcccagccg aggaagggca ccatgccgag
     3421 cgcggcgagg atcacgaaga agatcagcgc ctgcagcgcc cacaccagcg gcgcccgcat
     3481 gaagatgcgc cacccggcgg cgagccagtc gagggcgtgg ccgggcacca cgtcgccggg
     3541 ggcgggcggc ggcggatgcg ggcggcgggg cgggggcagg gtggcgttca tcggcgtcgg
     3601 gtcggggatg gggcgggcgt gacagagcgg gcggggcggc gtcagcgcgg caggggcggc
     3661 gccgcggcga tgcgcaggcg caggatgtcg cggtactcgt tcgggtcctt caccagcacc
     3721 atctcgcccg cgcgcggcag gtggaagtcg gccgcgcgcg acatccagaa gcgcagcgcg
     3781 gccgcgcgca gcatggccgg ccaggcggcg cgctcggcgt cggtgaaagg gcgctcggcg
     3841 tggtaggcgt cgagcagcgc ggcggcacgc tcggcatcca gcccgccgtc gggggtggag
     3901 caccagtcgt tgacggtgac ggcgacgtcg aacagcagcg cgtcgtagcc ggcgaagtag
     3961 aagtcgatga cgccgccgat gcgcaccccg ccgtcggcat cggtgtccca tagcacgttg
     4021 tcgcggaaga gatcggcgtg gatcgcgccg gcgggcaggg cggcgacgtc gatcgtggcc
     4081 tggaaggcga tctcggcgtc gagcagggtc tgttcgtcgg cgggcaggaa gggccgcagc
     4141 acctgtgcgg tggcggcgcg ccaggcggcg ccgcgcgggt tttcctggcg gcggccgtag
     4201 gactggccgg ccaggtgcag gccggcgagc atggcgccga ccctcccgca gtgcgccagg
     4261 ccggggctca tctcggagcg gcccgagagc cgtaccacca gtgcggcggg gcgctcctgc
     4321 agggtgccga ggtattcgtt gtggcggttg gcgatcgggc ccggcacggg caggccgtgg
     4381 cgcgacaggt gcgccatcag gtgcaggtag tagggcagct cggcgcgcgg gatgccctcg
     4441 aagagcgtca gcacgtagcg cccgagcgtg gtggtgacga aaaagttgct gttctgcacg
     4501 ccggccgaga tgccctcgag ttccaccagg cggccgatgg cgtagtcctt gagccagtcg
     4561 gcgagcacgg attcgggaac gggggtgaag acggacatgc ggactccggg taagacggtc
     4621 ggatggggtc aggcggcctg ggccgccggg atgtcgatgc gggctgcggc cgcgcggtgc
     4681 gctgcagcct tcacgacctg ccaggcggcg agcttggcct ccagcccgcg cgcggcgtgt
     4741 gccgggctgg tcgagggcag gcgggtgaag tgcagcggcg gcagcgcgat gcggccgcgc
     4801 acatggcggc ggaaggcggt ctcggcggcg gtgccgttga agaagacgtg gccgatgtcg
     4861 gggcaggcgg cgaagaggcc ggcgaagtcg ttggcctcga tgctgtcggg cgcgatcgcg
     4921 ctgtcgaggc tgccggcgcg ctcgcagcgg ccgatgacgt cccacagcgc gacgccggcg
     4981 gcggcgaggc gctcgagccg ctgcgcgtag gggagttccg ctccggcgcc gaagagcgcg
     5041 cccatgatcg gccagaaggc gttgcgcgga tgcgcgtaat actgcgcggc ggcgagcgag
     5101 gccgtgccgg gcatgctacc gagcaccagc acgcgcgcgt cggcgcggaa cagcggggca
     5161 aagctgcgca ccagcggtgc ggcggtcgtg ctcaccagga cttgatcacc cacatcggca
     5221 cggcgagttc gggcgtgccg tcgtggcgca ccatctggcc gttgccttcc ttgtcgatca
     5281 ggtagtaggg cacgccgtgg ggcggtgtga ccttgaccat gtagagcttg ccgcgcaggc
     5341 ggtattcggt gacggtgtcc tcgccgcgct tgacgatggt gacctcgggt tcgtcggctg
     5401 cgccctcggc gggcatcggc ggcggctcgg ggatcggttc gaggttcgcg ggctgctggg
     5461 cgaaggccgg cacggcggcg gccagccaca ggacgatcag ggtgcggcgc atgatgttct
     5521 ccggaagatg ctgcaattct agccggtaat gcggcactca aaggttcagc atcatctcgc
     5581 gctcggccgg aaggggctgg aaaccgcgcc gctcgtaatg cttgaagatg gcctcgacca
     5641 cctcctcggg ctcgtcgatc acctgcacca ggtcgaggtc ttcggcgttg atcatgcctt
     5701 cggcgaccag gcgctcgcgc agccagtcga gcaggccctg ccagaacggg cggtgcacca
     5761 ggatgatcgg aatcttgcgg ctcttcctgg tctggatcag cgtcatcgcc tcgagcagct
     5821 cgtcgagggt gccgaagccg cccggcatca ccacgtaggc ggtggcgaac ttcacgaaca
     5881 tgaacttgcg cgcgaagaag tgctggaagg tctgcgagat gtcctggtag gggttggcgg
     5941 cctgctccag cggcagctgg atgttgagcc ccaccgaggg actcttgccg aagaaggcgc
     6001 ccttgttggc cgcttccatg attcccggac cgccgcccga gatcaccgcg aagcccgaat
     6061 ccgacagcag gcgggcgatc ttttcggtga gcatgtagta cttgtggtcg ggcggcgtgc
     6121 gtgcgctgcc gaagatcgag accgccggcc ggatcgccgc caggcgttcg gtcgcctcca
     6181 cgaactctgc cataatcccg aagatccgcc aggattcgcg cgcgttgaag cgcggtgcgg
     6241 tgctgtcggg cacgctgcgg gggagtttgt ctttcgcggt catgttgttg ttctctagga
     6301 ttcggaatgc ccaccctgct gctcgtcgac ggctccagct atctctatcg cgccttccat
     6361 gccctgcccg acctgcgcaa ttcgcagggc gagccgacgg gcgcgatccg gggggtgctg
     6421 tcgatgctaa ggcggctgga aagcgactac aaggcagaat ttcgcgcctg cgtcttcgac
     6481 gccaagggca agaccttccg cgacgactgg tatcccgaat acaagtccca ccgcccgccg
     6541 atgcccgacg acctgcgcgc gcagatcgcg ccgctgcacg aggcggtgcg ggccgagggc
     6601 tggccgctgc tgtcggtgga gggcgtggaa gcggacgacg tcatcggcac gctcacccgc
     6661 ctggcgctcg agcgcggctg ggaggtggtg atctcgaccg gcgacaagga cctcacccag
     6721 ctcgtgcgcc ccggcgtgcg ctgggtcaac acgatgagcg aggaagtgct cgacgaggcc
     6781 ggcgtggcgg ccaagttcgg cgttccgccg gagcgcatcg tcgactacct cgcgctggtg
     6841 ggcgacacgg tggacaacgt gccgggcgtg gagaagtgcg gtcccaagac cgcggtgaag
     6901 tggctgaccg agtacggcac gctcgacaac ctcgtcgcca acgccgacaa ggtgggcggc
     6961 aaggtcggcg agaacctgcg caggcatctg gactttctgc cgctgggccg gaagctggtg
     7021 acggtggcga cggatgtgga gctgccggtg gggctggacg agctgccggc gcgcgcggac
     7081 gacaaggcgg cgctgcgcgc gctctacgag cgcttcgagt tcaagtcatg gctgaaggat
     7141 ctggatggcg gtgcggagag tgtcgccgcc tcgaaggccg cgtccgatgc gcgccgcttc
     7201 gaccgccagg aaaatcgggg acagaccccg atttccgagg ctgcgaccac gacgccaggg
     7261 gagggcgggg tgtgcgcaat ctccgcggat gagcaaatga aagacctgac cccggtctat
     7321 ctttccatcc tcgactggcc gaccttcgac gcctggctgg cgaagatctc cgctgccgag
     7381 ctcaccgcct tcgacaccga gaccaccagc ctcgacccga tggcggccag gctggtcggg
     7441 atgtccttcg cggtcgagcc gggcgaggcg gcctacctgc cgctggcgca ccgcggtgcc
     7501 gcgcagttgc cagaggcgcc ggagcagttg cccctggacg aggtgctcga caggctcagg
     7561 ccctggttcg aatccgaccg gcacgccaag ctcgggcaga acctcaagta cgacgcccac
     7621 gtgctcgcca accacggtat cgcgctgcgc ggcatcgcgc acgacaccct gctcgaatcc
     7681 tacgtgctgg agagcgacaa ggcgcacgac atggattcgc tcgccaagcg ccatctcggc
     7741 ctcgcgacga tcccttatac ggaggtctgc ggcaagggtg ccaagcagat cggcttcgac
     7801 gaggtcgatg tcgcgcgcgc caccgaatac gccgccgagg acgccgacgt caccctgcgc
     7861 ctgcaccagc acctgtgccc gcagctccag gccctgccgc agctcgaagc gctttaccgc
     7921 gagctcgaga tgccggtgct cggcgtgctg cagcgcatgg aacgcaccgg ggtgctgatc
     7981 gaccccttcc tgctcggcca gcactcggag gagctcggcc gccgcctgta cacgctcgag
     8041 ggcgaggcac atgcgctcgc cggccagccc ttcaacctcg gctcgcccaa gcagctcggc
     8101 gagatcctgt tcggcaagct cggcctgccc gtggtcaaga agaccgccac cggccagccc
     8161 tccaccgacg aggaggtgct ggagaagctc gccgaggact accccttgcc caagctcctg
     8221 ctcgagcacc gcggcctgtc caagctcaag agcacctacg ccgacaagct gccgcgcatg
     8281 gtcaatccgc gcaccggccg cgtgcacacc agcttctcgc aggccaccgc ggtcaccggc
     8341 cggctgtcga gctccgagcc caacctgcag aacatcccga tccgcacgcc cgaaggccgc
     8401 cgcatccgcg ccgccttcat cgc
//
LOCUS       DAOQBS010000131         8038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_8548_length_8038_cov_15.882125, whole genome shotgun sequence.
ACCESSION   DAOQBS010000131 DAOQBS010000000
VERSION     DAOQBS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8548_length_8038_cov_15.882125"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKN25_12650"
     CDS             <1..252
                     /locus_tag="PKN25_12650"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNV91794.1"
                     /translation="YGEYVTGPEVINEQSRAAMRNALKRIQTGEYAKMFILEGKTNYP
                     SMTAYRRLNADHQIEKVGGQLRDMMPWIKAKALVDKSKN"
     gene            409..1299
                     /gene="pssA"
                     /locus_tag="PKN25_12655"
     CDS             409..1299
                     /gene="pssA"
                     /locus_tag="PKN25_12655"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNV91795.1"
                     /translation="MTILESTEATPKVPDNEHAPLPLSDKRRRGIYILPNLFTTAALF
                     AGFYAIVQAMNQRFEYAAVAIFVAMVLDGLDGRIARLTNTQSEFGAEYDSLSDMVSFG
                     AAPALVMYEWALQDLGKLGWVAAFIYCAGAALRLARFNTNIDVVDKRYFQGLPSPAAA
                     ALVAGMVWVGIDNGFAGNELRWYAAVLTIFAGVSMVSNVLFWSGKSINLRKSVPFIVL
                     IALVLAFALISSYPPGILFGLFLAYAGSGYVVAALRWRRRRTHRAEPAAPQAAAYTAQ
                     AGDAAAAPVEGASSDPGSKP"
     gene            1296..2831
                     /locus_tag="PKN25_12660"
     CDS             1296..2831
                     /locus_tag="PKN25_12660"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNV91796.1"
                     /translation="MKDKLIIFDTTLRDGEQSPGASMTRDEKLRIARQLERMRVDVIE
                     AGFAAASNGDFEAVRSIAEAIKESTVCSLARANEHDIRRAGEAIRPAARGRIHTFIAT
                     SPIHMEKKLRMTPDQVVEQAVKAIGWAREYTDDVEFSAEDAGRSEIDFLVRIFEAVID
                     AGAKTINVPDTVGYNVPEQFAATVRTLIERVPNSDKVVWSVHCHNDLGLAVANSLAAV
                     RTGARQVECTINGLGERAGNASLEEIVMAVRTRADIFPVETGVDTTQIVPASKLVSQV
                     TGYPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWGANKLVLGKLSGR
                     NAFRARLQELGIEIESEEHLNQAFARFKELADRKSEIFDEDLTALMRDEAVTPAPEQY
                     RLVSTRFHSETGETPVAEITLSIGGAEKQARANGSGPVDAAFKAIETVATTGSELLLY
                     SVNAITSGTDAQGEVTVRLSRDGRVMDGQGADTDIIVASAKAYLNALNKLHVGAERLN
                     PQV"
     gene            complement(2893..3240)
                     /locus_tag="PKN25_12665"
     CDS             complement(2893..3240)
                     /locus_tag="PKN25_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HNV91797.1"
                     /translation="MNARMLSVVSSVALLALIALCVIWEGWLAPLRPGGSWMMLKALP
                     LLPAVFGVLRGRRYTSQWLSMLSLLYFTEGVLRATDPGLTGVFALVEVFLAVVLFTST
                     MLHARATAPSRAQ"
     gene            complement(3237..3836)
                     /gene="wrbA"
                     /locus_tag="PKN25_12670"
     CDS             complement(3237..3836)
                     /gene="wrbA"
                     /locus_tag="PKN25_12670"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNV91798.1"
                     /translation="MHEILVLYYSHRGSVRALAEHIAAGIHQVPGAVARVRTVPRVSA
                     VCEAVEDDIPASGPPYVEVADLEECVGLALGSPTRFGNMAAPMKYFLDGLAGAWLNGA
                     LEGKPGCVFTSSSSLHGGQESTLLSMMLPLFHHGMLVLGLPFSEPALGETRSGGTPYG
                     ASHVSGSEGDSAPSAEEIALAQAQGRRLAEVAMRLAGSR"
     gene            complement(3923..5437)
                     /locus_tag="PKN25_12675"
     CDS             complement(3923..5437)
                     /locus_tag="PKN25_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91799.1"
                     /translation="MEAPSTPSDAEQSLLRWLDSEPADAVEDDLAALRARMHDAFGGT
                     AAGPATSDALLCALAERMGDIGGRLRPLLLARQLPVDVPLFEANEQLCVLALDVATAV
                     SAAAGRAGRARPGGGAELCEYAIGVVTEIFVLSALAAAAPPAGTWRLAHDFAAHHEVA
                     AAAADDPLRCAYLRLLAVAVAQPEGLTGREIAWLYDFLDARVLGAAVLARGTGDTSAS
                     AWWIDLGADLGPQSVARRPAPSESVLGGDLWSFNPRPMAQKLGECIEWVTARMTAAEL
                     AGMQGEVESLDAEEALFPPGLSPLETVALLRRLRSHWMAAPVRGQPRRQHQYVVQVGI
                     GLQSVWDLGSGGRDDPALLQEWMVLNESPGGLAIMSVAGVRGEIEAGGALALRRKPEQ
                     AWSVCIVRWVRSDCPGQVEVGLQIIGQSFHPVMVGFRGRAVQQLSPALALPIMEPLRR
                     HPAMLAVAGTYASRRFVFVRGTQTVYVAQARALGLDLQTPSVELFQYEIDPYPI"
     gene            5633..5884
                     /gene="rpsP"
                     /locus_tag="PKN25_12680"
     CDS             5633..5884
                     /gene="rpsP"
                     /locus_tag="PKN25_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004884679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNV91800.1"
                     /translation="MVVIRLARGGAKKRPFFNMVVADSRNRRDGRFIERVGFYNPIAS
                     GAEKGLVINAERLAYWQQNGAQLSPTVARLVKQAGTAAA"
     gene            5913..6431
                     /gene="rimM"
                     /locus_tag="PKN25_12685"
     CDS             5913..6431
                     /gene="rimM"
                     /locus_tag="PKN25_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300530.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNV91801.1"
                     /translation="MIVLGRIVAPFGVQGWIKIHPFGDDPASWRNMSHWWLCSDDDAP
                     AEAWKQYTLSACRPHGKGLVAALAEISDRGAAEAIDGFYIAAPREALPKPAEGEYYWG
                     DLVGLAVENEAGEAMGTVAGLISTGAHDVLQVREGEGEEGVERLIPFVAAYVLDVDLA
                     TRRIRVAWQKDW"
     gene            6530..7300
                     /gene="trmD"
                     /locus_tag="PKN25_12690"
     CDS             6530..7300
                     /gene="trmD"
                     /locus_tag="PKN25_12690"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNV91802.1"
                     /translation="MITLFPEMFAALTGSGITRRAIDRGLYALGFHNPRDFTSDPHRT
                     VDDRPYGGGPGMVMLAEPLMQAIRAARAAQERALGRSGRVIYLSPQGRRLEQEVVLEL
                     AAEPALVLLCGRYEGVDQRLIDREVDEELSLGDFVLSGGELPAMVLLDAIVRQLPGAL
                     NTADSAQEDSFADGLLDCPHYTRPERYEDERVPEVLLSGNHAAIRRWRLKQSLGRTWL
                     RRPDLLAARQLSKTELKLLEEFRQEQAGTQDALEPQRG"
     gene            7398..7805
                     /gene="rplS"
                     /locus_tag="PKN25_12695"
     CDS             7398..7805
                     /gene="rplS"
                     /locus_tag="PKN25_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNV91803.1"
                     /translation="MNLLQQLEQEEMARVSQGKTIPEFGPGDTVIVQVKVKEGNRERL
                     QAYEGVVIAKRNRGLNSSFIVRKISSGEGVERTFQTYSPLVAGVEVKRRGDVRRAKLY
                     YLRQRSGKSARIKEKLDYKAAAAKRAAQQQQEA"
BASE COUNT         1377 a         2899 c         2656 g         1106 t
ORIGIN      
        1 tacggcgagt acgtgaccgg gcccgaggtc atcaacgagc agtcgcgcgc tgccatgcgc
       61 aacgcgctca agcgtatcca gaccggcgag tacgccaaga tgttcatcct cgagggcaag
      121 accaactacc cgagcatgac cgcctaccgc cgcctcaacg ccgaccacca gatcgagaag
      181 gtcggtggcc agctgcgcga catgatgccg tggatcaagg ccaaggccct ggtcgacaag
      241 tcgaagaact gatcgccccg gcgttcggtc caagggccgg taccgcactc gcggtgccgg
      301 cctttttcat tgctgcgccc gatcgcccgc gcaagcgcac gcaccgcagg atcgtcatgc
      361 ggcggcgcgg gcgcttttgc agccggcacg gcgcgcgcgt acgctaaaat gacgatcctg
      421 gaatccacgg aggcgacgcc caaggtgccc gataacgaac acgccccgct gcccttgtcc
      481 gacaagcgcc gccgcggcat ctacatcctg ccgaacctgt tcaccaccgc agcgctgttc
      541 gccggcttct atgcgatcgt gcaggcgatg aaccagcgct tcgagtacgc cgcggtggcg
      601 atcttcgtcg ccatggtgct cgacggcctc gacggccgca ttgcccgcct caccaacacc
      661 cagagcgagt tcggcgccga gtacgactcg ctctccgaca tggtctcctt cggcgccgca
      721 ccggcgctgg tgatgtacga gtgggcgctg caggacctcg gcaagctcgg ctgggtcgcc
      781 gccttcatct actgcgccgg ggccgcgctg cgcctggcac gcttcaacac caacatcgac
      841 gtggtcgaca agcgctactt ccagggcctg cccagtcccg cggcggcggc cctggtcgcc
      901 ggcatggtgt gggtgggcat cgacaacggc ttcgccggca acgagctgcg ctggtacgcc
      961 gcggtgctga ccatcttcgc cggcgtctcc atggtcagca acgtgctgtt ctggagcggc
     1021 aagagcatca acctgcgcaa gagcgtgccc ttcatcgtgc tgatcgcgct ggtgctcgcc
     1081 ttcgcgctca tctccagcta cccgcccggc atcctcttcg gcctcttcct cgcctacgcc
     1141 ggctcgggct acgtggtcgc cgcgctgcgc tggcgccggc gccgcaccca tcgcgccgaa
     1201 cccgccgcac cccaggcggc cgcgtacacc gcacaagccg gcgacgccgc cgccgcgccc
     1261 gtcgagggcg cttcatccga ccccgggagc aagccatgaa agacaagctg atcatcttcg
     1321 acaccacctt gcgcgacggc gagcagagcc ccggcgcgtc gatgacgcgc gacgagaagc
     1381 tgcgcatcgc ccgccagctc gaacgcatgc gggtcgacgt catcgaggcc ggcttcgccg
     1441 ccgcctccaa tggcgacttc gaggcggtgc gctcgatcgc cgaggcgatc aaggaatcca
     1501 ccgtgtgctc gctggcgcgc gccaacgagc acgacatccg ccgcgccggc gaggccatcc
     1561 ggccggcggc gcgcggccgc atccacacct tcatcgccac cagcccgatc cacatggaga
     1621 agaagctgcg catgacgccc gatcaggtcg tcgagcaggc ggtcaaggcg atcggctggg
     1681 cgcgcgaata caccgacgac gtcgagtttt ccgcagagga tgccggccgc tccgagatcg
     1741 acttcctggt gcgcatcttc gaggccgtga tcgacgccgg cgcgaagacc atcaacgtgc
     1801 ccgacacggt cggctacaac gtccccgagc agttcgccgc caccgtgcgc acgctgatcg
     1861 agcgcgtgcc caactcggac aaggtggtgt ggtcggtgca ttgccacaac gacctcgggc
     1921 tggccgtggc caactcgctc gccgcggtgc gcacgggcgc gcgccaggtc gagtgcacca
     1981 tcaatggcct gggcgagcgc gcgggcaacg cctcgctcga agagatcgtc atggcggtgc
     2041 gcacccgcgc cgacatcttc ccggtcgaga ccggcgtcga caccacgcag atcgtcccgg
     2101 cctcgaagct ggtttcgcag gtcaccggct acccggtgca acccaacaag gccatcgtcg
     2161 gcgccaacgc cttcgcgcac gagtccggca tccaccagga cggcgtgctc aagcaccgcg
     2221 agacctacga gatcatgcgc gccgaggacg tcggctgggg cgccaacaag ctcgtgctcg
     2281 gcaagctctc cggtcgcaac gccttccgcg cccgcctgca ggagctcggc atcgagatcg
     2341 agagcgagga gcacctgaac caggccttcg cgcgcttcaa ggagctcgcc gaccgcaagt
     2401 cggagatctt cgacgaggat ctcaccgcgc tgatgcgcga cgaggcggtg accccggcgc
     2461 ccgagcagta ccgcctggtg tcgacccgct tccactccga gaccggcgag acgccggtgg
     2521 ccgagatcac cctgtcgatc ggcggcgccg agaagcaggc ccgtgccaac ggctccggcc
     2581 cggtggacgc cgccttcaag gcgatcgaga cggtggcgac gacgggaagc gagctgctgc
     2641 tctactcggt gaacgcgatc accagcggca ccgacgccca gggcgaggtc accgtgcgcc
     2701 tgtcgcgcga cggccgcgtg atggacggac agggcgccga caccgacatc atcgtcgcct
     2761 cggccaaggc ctacctgaac gcgctcaaca agctgcacgt gggagccgaa cggctcaacc
     2821 cgcaggtatg agggcgtaag gcgtcaggag cgaagggcgg gtgcggaact tgccgacccc
     2881 gccccgcccg cctcactgcg cgcgggaggg agccgtcgca cgggcatgca gcatcgtgct
     2941 ggtgaacaac acgaccgcga ggaagacctc gacgagggcg aacacgccgg tgagcccggg
     3001 gtcggtggcg cgcagcacac cctcggtgaa atacagcagc gacagcatcg acagccactg
     3061 cgaggtgtag cggcggccgc gcagcacgcc gaacaccgcc ggcagaagcg gcagcgcctt
     3121 cagcatcatc cacgagccgc ccggacgcag cggggcgagc cagccctccc agatcacgca
     3181 cagcgcgatc agcgccagca gcgcgacgct cgacaccacg ctcagcatgc gcgcattcat
     3241 cgcgaccccg ccaggcgcat cgccacctcg gcaaggcgcc ggccctgcgc ctgcgcgagc
     3301 gcgatctcct ctgcgctcgg cgcagaatcg ccctcgctcc cggagacatg actcgcgcca
     3361 tagggcgtgc cgccgctgcg cgtctcgccg agcgccggct cggagaacgg caggccgagc
     3421 accagcatgc cgtgatggaa gagcggcagc atcatcgaca gcagcgtgct ctcctgcccg
     3481 ccatgcaggc tggacgagga cgtgaacacg cagcccggct tgccctcgag ggcgccgttg
     3541 agccaggcac cggcaagtcc gtcgaggaag tacttcatcg gcgccgccat gttgccgaag
     3601 cgcgtcgggc tgccgagcgc caggccgacg cactcctcca ggtcggcgac ctcgacgtag
     3661 ggcggcccgg acgccgggat gtcgtcctcc accgcctcgc acacggcgga cacccgcggc
     3721 accgtgcgca cccgcgccac cgcgcccggc acctgatgga tgccggcggc gatgtgctcg
     3781 gcgagcgcgc gcaccgaacc gcggtggctg tagtagagga cgaggatttc gtgcatcgga
     3841 tgccagttcc ggaaggggag cggattatat cgcgcggcgt cgccggccac ccgcagcccg
     3901 aaggcgcacg cgggcaatgc gctcagatcg gatagggatc gatctcgtac tggaaaagtt
     3961 cgacgctcgg cgtctgcagg tcgagcccga gcgcgcgtgc ctgggcgaca tagaccgtct
     4021 gcgtcccgcg tacgaagacg aagcggcgcg aggcgtaggt gccggcgacc gcgagcatcg
     4081 ccggatggcg gcgcagcggc tccatgatcg gcagcgcgag cgcgggcgag agctgctgca
     4141 ccgcacgccc gcgaaagccg accatgaccg gatggaagga ctgcccgatg atctgcaacc
     4201 cgacctccac ctgccccggg caatcgctgc gcacccagcg cacgatgcac accgaccagg
     4261 cctgctcggg cttgcgccgc agggccagcg cgccgcccgc ctcgatctcg ccgcgcacgc
     4321 cggccacact catgatcgca aggccgcccg ggctttcgtt gaggaccatc cactcctgca
     4381 gcaacgcagg gtcatccctt cccccactgc ccagatccca caccgattgc aggccgatcc
     4441 cgacctgaac cacgtattga tgctggcgcc gcggctgccc ccgcaccggc gccgccatcc
     4501 agtggctgcg aaggcggcgc agcaaggcca ccgtctcgag gggtgagagg ccaggcggaa
     4561 acagcgcctc ctcggcatcc agcgactcga cttcgccctg catgcccgcg agttctgccg
     4621 cggtcatgcg cgccgtcacc cactcgatgc actcgcccag cttctgcgcc atcgggcgcg
     4681 gattgaagct ccacagatcg ccgccgagca ccgactccga gggcgccggc cgccgcgcca
     4741 cggattgcgg ccccaggtcg gcgccgaggt cgatccacca cgccgatgcg ctcgtgtcgc
     4801 cggtgccgcg cgcgagcacc gcggcgccga gaacacgcgc atcgagaaag tcgtacaacc
     4861 aggcgatctc gcggccggta agcccctcgg gctgtgcgac cgcgacggcc agcagacgca
     4921 ggtaggcaca gcgcagggga tcgtccgcgg cagccgcggc aacctcatgg tgcgccgcga
     4981 agtcgtgcgc caaccgccaa gtccccgcgg gaggcgcagc cgccgccagc gcgctcagca
     5041 cgaagatctc ggtcaccacg ccgatcgcgt attcgcagag ctccgctccg ccccccggcc
     5101 gcgcccttcc ggcgcgcccg gctgcagccg agacggccgt ggcgacatcg agcgcgagca
     5161 cgcacaactg ctcgttcgcc tcgaacagcg gcacgtccac cgggagctgt ctcgcgagca
     5221 gcaagggccg cagccggcca ccgatgtcgc ccatgcgctc ggccagcgcg cacaacaacg
     5281 cgtcgctcgt cgccggaccg gcagcggtgc cgccaaaagc gtcgtgcatg cgcgctcgca
     5341 gcgcggcgag gtcatcctcc acggcgtctg caggctcgct gtcgagccag cgcagcagcg
     5401 actgctcggc atcggacggt gtcgaaggcg cttccatggt ccccggagaa acattgctcg
     5461 ggtcacattc tatccccatc ccgcgcacac acgccgcaac actgcggaca cccggcgccg
     5521 gtacgcggaa gatgtcgagg aatcgctcgt cggccgcaat aaaaccttgt ccgcgacggg
     5581 cgactcccct ataatccgcg gtttttcgca tccaccgatc aggaacatcc agatggtcgt
     5641 cattcgcctt gcccgtggtg gcgccaaaaa gcgcccgttc ttcaacatgg tcgttgccga
     5701 ttcgcgcaac cgccgcgacg gtcgcttcat cgagcgcgtc ggcttctaca acccgatcgc
     5761 ctcgggcgcc gagaaaggcc tcgtgatcaa cgccgagcgc ctcgcctact ggcagcagaa
     5821 cggcgcccag ctctcgccca ccgtcgctcg cctggtcaag caggccggca cggccgcggc
     5881 ctgatcgact tcgatcagcg gccgagcggc gcatgatcgt actggggcgc atcgtcgccc
     5941 cttttggcgt tcagggctgg atcaagatcc atcccttcgg tgacgacccc gcctcgtggc
     6001 ggaatatgtc gcactggtgg ctgtgcagcg acgacgacgc gcccgccgag gcgtggaagc
     6061 aatacacgtt gagcgcctgc cggccgcacg gcaaggggct ggtggccgca ctggccgaga
     6121 tatccgaccg gggcgccgcc gaggcgatcg acggcttcta catcgccgcg ccgcgcgagg
     6181 cgctgcccaa gcctgccgag ggcgagtact actggggcga cctcgtcggc ctggcggtcg
     6241 agaacgaggc gggtgaagcg atgggcacgg tcgcagggtt gatctcgacc ggcgcgcacg
     6301 acgtgttgca ggtgcgtgaa ggcgaaggtg aagagggggt cgaacgcctg atccccttcg
     6361 tcgccgccta cgtgctcgac gtcgacctgg ccacgcggcg catccgggtc gcctggcaga
     6421 aagactggta acacgggact gtggaaggcc ggtgaatccg cagagcccgc aaggcgaaac
     6481 acagcaggcg agcccttcgg gcgttgcagg cggcccgcgt cgatacgacg tgatcaccct
     6541 cttccccgag atgttcgccg cgctcaccgg cagcggcatc acgcggaggg cgatcgatcg
     6601 cgggctgtac gcgctcgggt tccacaaccc gcgcgacttc accagcgatc cgcatcgcac
     6661 ggtggacgac cgcccctacg gcggcggccc cggcatggtg atgctggccg agccgctgat
     6721 gcaggcgatc cgcgccgcac gcgcggcgca ggaaagggcg ctcggacgta gcgggcgggt
     6781 catctacctg tcgccgcagg ggcgcaggct ggagcaggag gtggtgctgg aactggccgc
     6841 cgagcccgcg ctggtgctgc tgtgcgggcg ctacgaaggc gtggatcagc gcctgatcga
     6901 ccgcgaggtc gacgaagagc tttcgctcgg cgacttcgtg ctgtcgggcg gcgagctgcc
     6961 ggcgatggtg ctgctcgacg cgatcgtgcg ccagctgccc ggcgcattga acaccgccga
     7021 ttcggcgcag gaggattcct tcgccgacgg cctgctggac tgtccgcact acacgcggcc
     7081 ggagcggtac gaagacgaac gcgtgcccga ggtgctgttg tcgggcaacc atgcggcaat
     7141 ccgccgctgg cgcctgaagc agtcgctcgg ccgcacctgg ctgcgccgcc ccgacctgct
     7201 cgcggcgcgc cagctcagca agaccgaatt gaaactgctt gaagagttca ggcaggagca
     7261 agccgggacg caggacgcac tggaacccca gcgcggctga gcgtcctttc acaaacggcg
     7321 cgcatcaggt tgcgacctgc tctgcggcca ggccgacgca agaggcgaag gccgataaca
     7381 tcgacggagt atctgcaatg aacctgcttc agcaactcga acaggaagaa atggcccgcg
     7441 tctcgcaggg caagaccatc cccgagttcg gtcccggcga caccgtgatc gtccaggtga
     7501 aggtcaagga aggcaaccgc gagcgtctgc aggcctatga aggcgtggtc atcgccaagc
     7561 gcaaccgcgg cctgaactcc tccttcatcg tccgcaagat ctcctcgggc gagggcgtcg
     7621 agcgtacctt ccagacctac tcgccgctgg tcgccggcgt cgaggtcaag cgtcgcggtg
     7681 acgtgcgtcg cgccaagctg tactacctgc gccagcgctc gggcaagtcg gcgcgcatca
     7741 aggaaaagct ggactacaag gccgctgccg ccaagcgtgc cgcccagcag caacaggaag
     7801 cctgatcgct ccggcgttcg cttcatcgaa acgggccttc gggcccgttt tttcgttcac
     7861 cgcagccgcg cggcggggct tggcaggcgc ggccacgccg gcccgtcgat cgcgacttgc
     7921 gtcgctcctg cggcgcggct gcggcgctgg gagtccgttg caggagcgac gcgagtcgcg
     7981 attgcgacgc ctggacacgc gccgggacgc ctcctccgtg gccccctgct cagcgccg
//
LOCUS       DAOQBS010000132         7934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_8730_length_7934_cov_14.496764, whole genome shotgun sequence.
ACCESSION   DAOQBS010000132 DAOQBS010000000
VERSION     DAOQBS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8730_length_7934_cov_14.496764"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /gene="alaS"
                     /locus_tag="PKN25_12700"
     CDS             <1..1722
                     /gene="alaS"
                     /locus_tag="PKN25_12700"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNV91804.1"
                     /translation="GRGYVLRRIIRRAIRHGYKLGARAAFFHKMVPDLVAEMGSAYPE
                     LKDGERRIMDVLRQEEERFFSTIENGMAIVEAELAAMEAAGKKVFDGDTAFKLHDTFG
                     FPLDLTADICREREVTVDALAFDAAMARQKEQARAAGKFKMAANLEYDGPETTFHGYT
                     LLEEKGNILALYKDGAPVNELLEGEMGVVVLDHTPFYAESGGQVGDRGELRAAAGIFG
                     VEDTQKIQAAVFGHHGVVRTGKLAVGQGVVARVDVAARAATARNHSVTHLMHKALREV
                     LGAHVQQKGSLVDPDKTRFDFAHTAPMSAEEIREVEEIVNREILANAATQAEVMALEA
                     AQKSGAMMLFGEKYADEVRVLSIGTSKELCGGTHVARTGDIGLFKIVGEGGVAAGIRR
                     VEAITGENALRYAQEQERRVQGMSALLKVQPDEVAERVAGILDNVRALEKELARLKSK
                     LAASQGDELVSQAVEIKGIRVLAALLEGADAPTLRDTMDKLKDKLKSAAIVLAAVADG
                     KVSLIAGVTADHTAKVKAGELVNFVAQQVGGKGGGRPDMAQAGGTQPENLAAALAGVK
                     DWVAGKL"
     gene            1931..2173
                     /locus_tag="PKN25_12705"
     CDS             1931..2173
                     /locus_tag="PKN25_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNV91805.1"
                     /translation="MQSVNMLQAKSSLSRLVEAIESGREREIVIARNGRPAARLVPME
                     SASVGPRIGVAKGRFEVPDDIDASNEDVARLFLAGG"
     gene            2176..2562
                     /locus_tag="PKN25_12710"
     CDS             2176..2562
                     /locus_tag="PKN25_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV91806.1"
                     /translation="MNLLLDTHVALWAITDHPRLPQAARDLILSPKTTVWVSVASVWE
                     IAIKHALGRGDMPVSSRDAVCYFTASGYRLLAIEPEHVVAVETLPAHHQDPFDRLLVA
                     QALVEPMRLVTHDRFVALYSDTIIKI"
     gene            2579..2983
                     /locus_tag="PKN25_12715"
     CDS             2579..2983
                     /locus_tag="PKN25_12715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNV91807.1"
                     /translation="MRRLLSLLALLPLAAAIAAGPQTTTLPSGVTVETYRVGETGAPP
                     TLADVVRVHYQGTLADGKEFDSSYSRGRPAELPLEALVPCWKEVLQQMRPGGAVRIVC
                     PPETAYGSRGVPGRIPPDSTLNFVIELLEVKR"
     gene            complement(3000..3806)
                     /locus_tag="PKN25_12720"
     CDS             complement(3000..3806)
                     /locus_tag="PKN25_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNV91808.1"
                     /translation="MTVTLQRLVESAAFQRFIVMVIVINAITLGLETSPTAMQQAGGL
                     LLALDRAALAVFVVELALKLYVYRLRFFRSGWNVFDFTIVAITLAPTGEGLQVLRSLR
                     ILRALRLVSVVPSMRKVVNALLRAIPGMSSVLTLLLLVFYVAAVMSTKLFGASFPEWF
                     GSIGESFYTLFQVMTLESWSMGIARPVMEAYPYAWIFFVLFILLTTFAVLNLFIAIVV
                     DSMSAVEHEEQEQTRELVGVDHDAVLAELRAIRAELAALRGAGGERPPTA"
     gene            complement(4012..4989)
                     /locus_tag="PKN25_12725"
     CDS             complement(4012..4989)
                     /locus_tag="PKN25_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNV91809.1"
                     /translation="MESIGTWWMWAAFAAIVGTMLLVDLFAFGGGKQHRVSLKEAAGW
                     SLAWVTVAMLFAAGLWWYLDGTLGRAVANEQTTAFVTGYLVEKSLAVDNVFVWMMIFS
                     FFAVPLELQRRVLLYGIVGAIVLRTIMIFAGSWLISEFHWLLYVFGAFLIVTGIKMAW
                     FSEHDPDLDKNPLIRWIRNHYPITEKLEGERFFVVRDGVRMMTPLFLALVLVEISDVI
                     FAVDSIPAIFAITTDPFIVLTSNLFAILGLRAMYFLLADLGDRFELLKYGLAAILVFI
                     GTKMLIVEWVKIPVLVSLSVVATILACSVFASLWYTARKEKEAARINAG"
     gene            5412..6755
                     /locus_tag="PKN25_12730"
     CDS             5412..6755
                     /locus_tag="PKN25_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNV91810.1"
                     /translation="MEILFLIGLIVLNGVFAMSEIALVTARRARLARLAEEGDGAAEV
                     AIKLGEDPTRFLSTIQIGITSIGVLNGIVGEAALAGPLSDWLQVLGMEQRVSEIGSTV
                     LVVVVITYVSIVVGELVPKRIGQIDPEGIARLVARPMNVLSIASRPFVYLLAGSTALL
                     LRIMGQRESTGPSVTEEEIHAMLVEGSEAGVIEKSEHDMVRNVFRLDDRQIGSLMVPR
                     ADIVSLDLERPLEENLELVASSSYSSFPVCRGGLDDILGIASAKKLFNQSLRGEPIDL
                     ARELQPAVYVPESLTGMELLDQFRSSGTYTVFVIDEYGEIQGMVTLHDVIESVTGEFL
                     PHDTEEAWAVQREDGSWLLDGLIPIVELKDRLGIKTVPEEEKGRYHTLSGMVMWLLGR
                     LPGTGDIATWENWRFEVIDLDGKRIDKVLASRLPEPADEIGAEQEASPEDRGETA"
     gene            6752..7873
                     /locus_tag="PKN25_12735"
     CDS             6752..7873
                     /locus_tag="PKN25_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNV91811.1"
                     /translation="MIDQVAEKVVRRVILGFLLGGLLLLSYAVLHLFIVPVAWAVIIA
                     YATWTPYRQLRSRLPRYPTISALLMTLLLSAAFVLPALWIGMLLRTEVGVAIAAVTAQ
                     IREGAFVLPEFVRTLPWIGDDLQAMVGELTREPEALRAQLTEWVRQGSDLALTLIGDV
                     GRNAAKLGFALITVFFLYRDGERVLEQVVAVLRRFLGERLDPYLSAVGGMTKAVVWGL
                     IATAIGQGFVAGLGYWWAGVPAPVLMGAITAVIAMIPFGTPFAWGSIGAWLLLTGNTV
                     EGIGLLLWGALVVSWVDNLVRPLVISNATRIPFLLVMFGVLGGLAAFGLVGLFLGPVV
                     LAVLMAVWGEWLEESEFARLAAIGAPAGAREEVERGGRS"
BASE COUNT         1304 a         2596 c         2805 g         1229 t
ORIGIN      
        1 ggccgcggct acgtgctgcg ccgcatcatc cgccgcgcca tccgccacgg ctacaagctc
       61 ggcgcgcgcg ccgccttttt ccacaagatg gtgcccgacc tggtcgccga gatgggcagc
      121 gcctatcccg agctgaagga cggcgagcgc cgcatcatgg acgtgctgcg ccaggaagaa
      181 gagcgcttct tctccaccat cgagaacggc atggcgatcg tcgaggccga gctcgccgcg
      241 atggaagcag cgggcaagaa ggtcttcgac ggcgacaccg ccttcaagct gcacgacacc
      301 ttcggcttcc cgctcgacct caccgccgac atctgccgcg aacgcgaggt caccgtcgac
      361 gcgctcgcct tcgatgccgc gatggcgcgc cagaaggagc aggcgcgcgc cgccggcaag
      421 ttcaagatgg ccgccaacct ggaatacgac ggccccgaga ccaccttcca cggctacacg
      481 ctgctggaag agaagggcaa catcctcgcg ctgtacaagg acggcgctcc ggtcaatgag
      541 ctgctcgagg gcgagatggg tgtggtcgtg ctcgaccaca ccccgttcta tgccgagtcc
      601 ggcggccagg tcggcgaccg cggcgagctg cgcgccgccg ccggcatctt cggcgtggaa
      661 gacacgcaga agatccaggc cgcggtgttc ggccatcacg gcgtggtgcg caccggcaag
      721 ctggcggtcg ggcagggcgt ggtcgcgcgg gtggacgtcg ccgcgcgcgc cgccaccgcg
      781 cgcaaccact cggtcaccca cctgatgcac aaggcgctgc gggaagtgct tggcgcccac
      841 gtgcagcaga agggctcgct ggtcgatccc gacaagaccc gcttcgactt cgcccacacc
      901 gcgccgatga gcgccgagga gatccgcgag gtcgaggaga tcgtcaaccg cgagatcctc
      961 gccaacgccg ccacgcaggc cgaggtgatg gcgctggagg cggcgcagaa gtcgggcgcg
     1021 atgatgctgt tcggcgagaa gtacgccgac gaggtgcgtg tgctgtcgat cggcacgtcg
     1081 aaggagctgt gcggcggcac ccacgtggcg cgcaccggcg acatcggcct gttcaagatc
     1141 gtcggagagg gcggtgtcgc cgccggcatc cgccgcgtcg aggcgatcac cggcgagaac
     1201 gcgctgcgct acgcccagga gcaggagcgc cgcgtgcagg gcatgtcggc gctgctcaag
     1261 gtccagcccg acgaggtcgc cgagcgcgtc gccggcatcc tcgacaacgt tcgtgcgctg
     1321 gagaaggaac tggcccgcct caagagcaag ctcgcggcca gccagggcga cgagctggtg
     1381 agtcaggcgg tcgagatcaa aggcatcagg gtgctcgccg ccctgctcga aggcgccgac
     1441 gcgccgaccc tgcgcgacac catggacaag ctcaaggaca agctcaagtc ggccgcgatc
     1501 gtgctcgccg cggtcgccga cggcaaggtc agcctgatcg cgggcgtcac cgccgaccac
     1561 accgccaagg tcaaggctgg cgagctggtc aacttcgtcg cccagcaggt cggcggcaag
     1621 ggcggcggcc gccccgacat ggcccaggcc ggcggcaccc agccggagaa cctggcggcg
     1681 gcgctggcgg gggtgaagga ctgggtggcc gggaagctgt aagcgcgatc ttcagcgcgg
     1741 cgagcgcgag cggcgcgcgt ttgcgatccg gccttgcatg caggacgacg cccggcgatt
     1801 gccgggcgtc gtttttcgag acgggctccg gcgcaggcgg tgccgtcggt gcaatggaga
     1861 atcccgcgaa cgctcgtttg caggaaaaga ttagccagac tagactaaac aagtgtccga
     1921 ggaggcctcc atgcaatccg taaatatgct gcaggcaaag tcatccttgt cccgcctggt
     1981 ggaggcgatc gagtccggcc gggagcgcga gatcgtcatc gcccgcaacg gccgccctgc
     2041 cgcgcggctc gttccgatgg agagtgcgtc cgtggggccg cgcatcgggg tggccaaggg
     2101 caggttcgag gtgcccgacg acatcgacgc cagcaacgag gacgtcgcca ggctgttcct
     2161 cgccggaggc tgatcttgaa tctgcttctg gatacgcatg tcgcgctgtg ggccatcacc
     2221 gaccacccca ggctgccgca ggccgcgcgc gatctgatcc tgtcgcccaa aacgacggta
     2281 tgggtcagcg tcgcaagcgt ctgggagatc gccatcaagc atgcgctcgg gcgtggagac
     2341 atgccggtgt ccagccggga tgccgtgtgc tatttcaccg cctcggggta tcgcctcctg
     2401 gcgatcgagc ccgagcacgt cgtggcggtc gagaccttgc ccgcgcatca tcaggaccct
     2461 ttcgaccgtc ttctggtggc acaagcactg gtcgagccga tgcgcctggt cacgcacgat
     2521 cgtttcgtgg cgctctacag cgacaccatc atcaagatct gaatctccgg agtccgacat
     2581 gcgccgactg ctttccctcc tcgccctgct tccccttgcc gctgcgatcg ccgccgggcc
     2641 gcagaccacg accttgccct cgggcgtcac cgtcgagacc taccgcgtgg gcgagaccgg
     2701 cgcgccgccg acgctggccg acgtggtgcg ggtgcattac cagggcacgc tcgccgacgg
     2761 caaggagttc gacagctcct acagccgcgg ccgcccggcg gagctgccgc tggaggccct
     2821 ggtgccgtgc tggaaggaag tgctgcagca gatgaggccg ggcggcgcgg tgcgcatcgt
     2881 atgtccgccg gagaccgcct acggcagtcg cggcgtgccc ggccgcatcc cgcccgacag
     2941 cacgctgaac ttcgtgatcg agctgctcga ggtcaagcgc tgaggcggtc ggtgccgcgt
     3001 caggcggtgg gtgggcgctc gccaccggcc ccccgcagcg cggccagctc ggcgcggatc
     3061 gcgcgcagtt cggcgagcac cgcgtcgtgg tccacgccga ccagctcgcg ggtctgctcc
     3121 tgctcctcgt gttcgaccgc gctcatcgaa tcgaccacga tggcgatgaa gaggttgagc
     3181 acggcgaagg tggtgagcag gatgaagagg acgaagaaga tccacgcgta gggataggcc
     3241 tccatgaccg ggcgggcgat gcccatcgac cacgactcca gggtcatgac ctggaacagg
     3301 gtgtagaagg attcgccgat gcttccgaac cactccggga agctcgcgcc gaagagcttg
     3361 gtgctcatca ccgcggcgac gtagaacacg agcagcagca gggtgagcac cgagctcatg
     3421 ccggggatgg cgcgcagcag cgcattgacg accttgcgca tcgagggcac caccgacacc
     3481 aggcgcagcg cgcgcaggat gcgcaaggag cgcagcacct gcaggccttc gccggtgggg
     3541 gcgagggtga tcgcgacgat ggtgaagtcg aacacgttcc agccgctgcg gaagaagcgc
     3601 aggcggtaga cgtagagctt gagcgcgagc tcgaccacga acaccgcgag cgcggcgcgg
     3661 tcgagcgcga gcagcagtcc gcccgcctgt tgcatggcgg tgggcgaggt ctcgaggccg
     3721 agggtgatcg cgttgatgac gatcaccatg acgatgaagc gctggaaggc ggcggattcg
     3781 acgaggcgtt gcagggtgac ggtcatgtcg ggtcgggggg aggtaggggg gcggtagggg
     3841 taggggattc agggggggcg gcacctgctt ttccggcggg atcgcgactt gcgtcgctcc
     3901 taccgtgcag cccgacattt cgggctggtt ctcgtgggag cgacgcaagt cgcgatggcg
     3961 ggatgggggc ggcgacgcgg cgcccggcgc ccccaacccg cccgctgcgc gtcagcccgc
     4021 gttgatcctg gcggcttcct tctccttgcg cgccgtgtac cacaggctcg cgaacaccga
     4081 gcaggccagg atggtggcga ccaccgacaa cgagaccagc accgggatct tcacccactc
     4141 cacgatcagc atcttggtgc cgatgaagac caggatggcc gccagtccgt acttgagcag
     4201 ctcgaagcgg tcgccgaggt cggcgagcag gaagtacatc gcgcgcaggc cgaggatggc
     4261 gaacaggttc gaggtcagca cgatgaaggg gtcggtggtg atcgcgaaga tcgccgggat
     4321 gctgtcgacc gcgaagatca cgtcggagat ttcgaccagc accagggcga gaaagagtgg
     4381 cgtcatcatg cgcacgccgt cgcgcacgac gaagaagcgc tcgccctcga gcttctcggt
     4441 gatcgggtag tgattgcgga tccagcggat gagcgggttc ttgtccaggt cggggtcgtg
     4501 ctcggagaac cacgccatct tgatgccggt gacgatcagg aaggcgccga agacgtaaag
     4561 cagccagtgg aactcgctga tcagccagct gcctgcgaag atcatgatcg tgcgcagcac
     4621 gatcgcgccg acgatgccgt acagcagcac gcggcgctgc aactcgagcg gcaccgcgaa
     4681 gaagctgaag atcatcatcc acacgaagac gttgtcgacc gccagcgact tctcgaccag
     4741 gtagccggtg acgaaggcgg tcgtctgctc gttggcgacc gcacgcccga gcgtgccgtc
     4801 gagataccac cacagcccgg cggcgaacag catcgccacg gtgacccagg cgagcgacca
     4861 gcctgccgcc tccttcaacg acacccggtg ttgcttgccg ccaccgaagg cgaacaagtc
     4921 gacaagcagc atcgttccca cgatggcggc aaaggccgcc cacatccacc acgttccgat
     4981 cgattccatc gcgcgctccg cagaaaacaa aaaaggtctg ttcccgacgg acacagaccc
     5041 tggcgcgctg cgatgcacgg accttgccca tcgggcaagg tctcgcttgc aacgaacgaa
     5101 tggatccgtt cgttgccccc ggcaccggga gacggtctga agaccgatct gccgtattga
     5161 cgatgccgga gcggaaaagc tactcccctt gatgaacggg attgtgtctg ttgcactgcg
     5221 ggagtgtcaa cgccggcgtt tcgggttgcc gcgagcaagg ggttcgtggt gttgcgctgc
     5281 agcagcgggc gagcgtggcg cgtggtcgcg cggtcgacac cccgtgtccg ctggggcatc
     5341 atcggcggcg ttcggcccgc ccgtcatgcc cgggtgcccg ccggcgcccc tctctcaacc
     5401 acctcgaacc catggaaatc ctctttctga tcggcctcat cgtcctcaac ggcgtcttcg
     5461 ccatgtccga gatcgcgctc gtcaccgcgc gtcgggcacg gctcgcgcgg ctcgccgagg
     5521 agggcgacgg cgcggccgag gtggcgatca agctcggcga ggacccgacc cgcttcctgt
     5581 cgacgatcca gatcggcatc acctcgatcg gcgtgctcaa cggcatcgtc ggcgaggccg
     5641 cgctcgccgg cccgctctcc gactggttgc aggtgctcgg catggagcag cgggtgagcg
     5701 agatcggctc caccgtgctg gtcgtggtgg tgatcaccta tgtgtcgatc gtggtcggcg
     5761 agctggtgcc caagcgcatc gggcagatcg accccgaggg gatcgcccgc ctggtggcgc
     5821 ggccgatgaa tgtgctgtcg atcgcctcgc ggcccttcgt ctacctgctc gccggctcca
     5881 ccgcgctgct gctgcgcatc atgggccagc gcgagagcac cgggccgagc gtgaccgagg
     5941 aggagatcca cgccatgctg gtcgagggct ccgaggccgg cgtgatcgag aagagcgagc
     6001 acgacatggt gcgcaacgtg ttccgcctcg acgaccgcca gatcggctcg ctgatggtgc
     6061 cgcgcgcgga catcgtgtcg ctcgacctcg agcggccgct ggaggagaac ctcgagctgg
     6121 tggcgtcctc gtcctactcc agcttcccgg tgtgccgcgg cgggctcgac gacatcctcg
     6181 gcattgccag cgccaagaag ctcttcaacc agtccctgcg cggcgagccg atcgacctcg
     6241 ccagggagct gcagcctgcg gtgtacgtgc ccgagtcgct gaccggcatg gagctgctcg
     6301 atcagttccg ctcttccggc acctacaccg tgttcgtgat cgacgagtac ggcgagatcc
     6361 agggcatggt cacgctgcac gacgtgatcg agtcggtgac cggcgagttc ctgccgcacg
     6421 acaccgagga ggcctgggcg gtgcagcgcg aggacggctc ctggctgctc gacggcctga
     6481 tcccgatcgt cgagctcaag gaccgcctgg gcatcaagac cgtgccggaa gaggagaagg
     6541 ggcgctacca cacgctgtcg ggcatggtga tgtggctgct cggccgcctg ccgggcaccg
     6601 gcgacatcgc cacctgggag aactggcgct tcgaggtgat cgacctcgac ggcaagcgta
     6661 tcgacaaggt gctggcgagc cgcctgcccg agccggccga cgagatcggc gccgagcagg
     6721 aggccagccc ggaagatcgg ggcgagaccg catgatcgac caggtcgcgg agaaggtcgt
     6781 ccgccgcgtc atcctcggct tcctgctcgg cggcctgctg ttgctgagct atgcggtgct
     6841 gcacctgttc atcgtgccgg tggcgtgggc ggtgatcatc gcctatgcca cgtggacgcc
     6901 ttaccgccag ctgcgctcgc ggttaccgcg ctatcccacg atcagcgcgc tgctgatgac
     6961 gcttttgctg agcgccgcct tcgtgctgcc cgcgctgtgg atcggcatgc tgctgcgcac
     7021 cgaggtcggt gtcgcgatcg ccgcggtgac cgcgcagatc cgcgagggcg ccttcgtgct
     7081 gcccgagttc gtccgcacgc tgccgtggat cggcgacgac ctgcaggcga tggtgggcga
     7141 gctcacccgc gagccggagg cgctgcgcgc gcaactcacc gagtgggtgc gccagggcag
     7201 cgacctcgcg ctgacgctga tcggcgacgt cgggcgcaac gcggccaagc tgggtttcgc
     7261 actcatcacc gtgttcttcc tctaccgcga cggcgagcgc gtgctcgagc aggtggtggc
     7321 ggtgctgcgc cgcttcctcg gcgagcgcct ggacccctat ctctccgcgg tgggcgggat
     7381 gaccaaggcg gtggtgtggg gcctgatcgc gaccgcgatc ggccagggtt tcgtcgccgg
     7441 gctgggctac tggtgggcgg gcgtgccggc accggtgctg atgggggcga tcaccgccgt
     7501 gatcgcgatg atccccttcg gcacgccctt cgcgtggggc tcgatcggcg cctggctgct
     7561 gctcaccggc aacaccgtcg agggcatcgg gctgctgctg tggggcgcgc tggtggtgag
     7621 ctgggtggat aacctggtgc gtccgctggt gatcagcaat gccacccgca tccccttcct
     7681 gctggtgatg ttcggcgtgc tcggcgggct ggccgcgttc gggctggtcg gcctgttcct
     7741 cgggccggtg gtgctggcag tgctgatggc ggtgtggggc gagtggctgg aggagtcgga
     7801 gttcgcgcgc cttgcggcga tcggagcgcc cgcgggcgcg agggaggagg tggagcgcgg
     7861 cggtcggtcc tgagccgccg atcgcgactc gcgtcgctcc tacaggaaac gtcgaagcgc
     7921 gcggtcgccc cgta
//
LOCUS       DAOQBS010000133         7911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_8768_length_7911_cov_18.647276, whole genome shotgun sequence.
ACCESSION   DAOQBS010000133 DAOQBS010000000
VERSION     DAOQBS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8768_length_7911_cov_18.647276"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..524)
                     /locus_tag="PKN25_12740"
     CDS             complement(72..524)
                     /locus_tag="PKN25_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNV91812.1"
                     /translation="MKKAIALGILSLTLATAASAQVKPEDQIKFRQAGYSFMSWNMGK
                     IKANLEGEYNAQQVQAAATAIAGIAGSGMGALYGPGTEKDIGNLKTRVKPEFFQEMPN
                     VGKLATDFNNAAANLAKVAATGDKAAVQKAFADTGSTCKACHDKYRME"
     gene            796..2418
                     /locus_tag="PKN25_12745"
     CDS             796..2418
                     /locus_tag="PKN25_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV91813.1"
                     /translation="MIRFSDTPIWLRLTGAIWLMMVAAFGSMIVWETRVNRDMAIEQA
                     KDFAGTVNEMTMAGLTGMMITGTVAQRDVFLDQIKELSVVQDLEVIRGEAVSRVFGAG
                     GARDAARDADERLALVEGRATMRVDHDAEHGEHLRVVIPSLASPAWLGKDCIACHQVP
                     QGTPLGAVSMRISLDKANAAVNAFRNQSAMFAALVSIPLILFIYLFITRFVTRPLDGL
                     NRGLGRIAEGGGDLRLQLPVRGRDEIGRSARTFNDMMGTLGGLVRQVGDSASAVTEAA
                     SRLAADAEAVAASSQRQNESSVGAAGAVDEMVGSISAIAEHAEAVRERSHDSLERSRE
                     GQRSLERLVGEVGEVEAAVRQMADAVVAFVDSTQAISRMTSEVREIAEQTNLLALNAA
                     IEAARAGEQGRGFAVVADEVRKLAEKSARSAGEIDQITREVARRSSSVRGALDDGLGH
                     LASSRQMADAVMQVLTAANSLVVEVGEGLDRIAGATGAQRSASESVTARIGEIAGMAR
                     SNNDALAHTVRSARELGELALRLQESVSRFRF"
     gene            complement(2500..3969)
                     /locus_tag="PKN25_12750"
     CDS             complement(2500..3969)
                     /locus_tag="PKN25_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNV91814.1"
                     /translation="MRKLLTVTAVALALGTLFPQASALSAAHAQAAAPSMVSPNRFGL
                     PDFGDLVEQVGPAVVNIRVVQRAAQGTAGENPLANDPFYDFLRRFGVPMPGFPGQPGM
                     GPRARQGIGSGFIVSKDGYVLTNAHVVAGEDGDAALSEVTVTLIDKREFKAKVVGIDR
                     RTDVALLKLDASGLPAVKIGNPDQARVGEWVVAMGSPFGFDNTVTAGIISAKARRLPD
                     ETYVPFIQTDVAINPGNSGGPLFNLAGEVIGINSQIYSRSGGFMGISFAIPIDVAMNI
                     KDQLVSHGRVQRGRLGIAIQNVDKDLAQSFGLTDPRGALVASVEPGSAADKAGLQAGD
                     VVLAVDGRRIDDSAELPRVIGEKRPGTRVKLELWRDGRSREVAATLDELKAETVAGSA
                     PAPSQVERIGEQFGLSARALTAEETARLQLRGGVVVEGADGAAARAGLQRGDVILAIN
                     NQPVASVADLRAQLERAGKRFALLIQRGEARIFVPVRLE"
     gene            complement(4118..6154)
                     /locus_tag="PKN25_12755"
     CDS             complement(4118..6154)
                     /locus_tag="PKN25_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV91815.1"
                     /translation="MHLHRPPTALALLSLLAAGTVEASSADAFFEPLPVVLSASRLAQ
                     PLQDSPGAVTVIDADLIAATGYRELARVLRLVPGFQVGQERGNLQWVTYHGLGMDSPM
                     QIQLLVDGRASLTPSFTASAERALPGDIERIEVVRGSNSAAYGSNAFLGIVNIITDDA
                     SRHPGTRLKLATGAPGIRDASLRHAVQSGPLSVRIGAHHEEDEGFADLYDGRRTDVLN
                     LRADLQVGEHDALALNAGVAESTHELGYAGSIFDIAAQREGRDTNRSLHLRWRHGPED
                     DAIRVSAYWSRQSRDDLWRYDSAANSRTLANAFPERLRGTVGNSATFVQHNLEIERRE
                     RVDEATRLMWGAEWKRIEHDSPFWYHERRNHARDEHRLFANLELRTGTAFLWNAGGML
                     EQLQGDEARLAPRVFLNWQAAPRITWRIGHSRAWRQPDLFERNVDIRITDDKGELVQR
                     RQYPNPDLRPQRIDASEIGFLGVFGDSRSTLDVRLFRERIEDLIVRNAVPSDWPIEGK
                     LPEAISRGLGSTRWENHSGRVQLNGIETQLHLKPWRGTEFLLAHSLIDRDVDDPRIRR
                     NVAPYTASLSWLQRAGAWRSLLSVLRMGPIDAGFSYVPGFDYTVPSYTTVDWSVSRSL
                     RLFDMAVELRLTGINLLGRHQELANRPLQAQPEFAGRPANEVSRQAWLALETTF"
     gene            6284..7240
                     /locus_tag="PKN25_12760"
     CDS             6284..7240
                     /locus_tag="PKN25_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNV91816.1"
                     /translation="MTLTDLRYLVALAHERHFGRAAEKCHVSQPTLSVAVKKVEEELG
                     IQLFERSASEVKITETGRRIVAQAERVLMEASQIQEIAAAGKDPLAGPLRVGVIYTIG
                     PYLLPRLIPRVHQLAPRMPLIIQENFTARLAEALKRGELDVIIISLPFDEAGVVAQPV
                     YDEPFRVLMPADHPWNAESEIDPQHLADDQLLLLGAGNCFRDQVLEVCPSCRNIGGLQ
                     RTLEGSSLETIRHMVATGLGVTVLPSSAADEMATQNPLVAVRPFTMPEPSRRVALAWR
                     VTYPRNGAIDVLRAAILDSALPGVRPVGRAVPPGQVPWVDSR"
     gene            7357..7827
                     /locus_tag="PKN25_12765"
     CDS             7357..7827
                     /locus_tag="PKN25_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HNV91817.1"
                     /translation="MEIDIGIKEKDRAKIAEGLSRLLADSYTLYLTTHNFHWNVTGPM
                     FNTLHLMFETQYNELALAVDEIAERIRALGFPAPGTYKEFQKLTSMAEPEGVPNAQEM
                     IRLLVQGQEAVVKTARSIAPIADKASDEPTLDLLTQRMQVHEKTAWMLRSLLEG"
BASE COUNT         1179 a         2610 c         2870 g         1252 t
ORIGIN      
        1 cctaggcgcg catccgtgcc aggaacgaca aagccacccg caggtggctt tgtcgttcga
       61 tatctgacgc ctcactccat ccggtatttg tcgtgacagg ccttgcaggt gctgccggtg
      121 tcggcgaagg ccttctgcac cgcggccttg tcgccggtgg cggcgacctt ggcgaggttg
      181 gcggcggcgt tgttgaagtc ggtggcgagc ttgccgacgt tcggcatctc ctggaagaac
      241 tccggcttga cgcgggtctt gaggttgccg atgtccttct cggtgcccgg gccgtagagc
      301 gcgcccatgc ccgagccggc gatgccggcg atcgcggtgg cggcggcctg gacctgctgg
      361 gcgttgtact cgccctcgag gttggccttg atcttgccca tgttccagct catgaagctg
      421 tagccggcct ggcggaactt gatctggtcc tcgggcttga cctgggccga agccgcggtg
      481 gcgagggtaa gggagaggat gccgagggcg atcgcttttt tcatcgtgag cctcctgagt
      541 cgggggttga aggaaggaac aaaccttgcg gggaactgcg cagaccagga tgcgccgcgc
      601 cagttccatc gtgttgcatg ccgggatccg cctgccggca cccgacttat aacaaattcg
      661 gtcgatattc cttcgtccgc tcgtgttgcg gcgcggaaga agtgcgctca agccgggccg
      721 tgcgcggccg taaggaagga gtagaggctg cgtgtcccca cgccgccgac cgtccgcagg
      781 accgcgagaa ccgacatgat ccgattttcc gacaccccga tatggctgcg tctcaccggc
      841 gcgatctggc tgatgatggt ggccgcgttc ggcagcatga tcgtctggga aacccgggtg
      901 aaccgcgaca tggcgatcga gcaggcgaag gacttcgccg gcacggtcaa tgagatgacc
      961 atggccggac tcaccggcat gatgatcacc ggcacggtgg cgcagcgcga cgtcttcctc
     1021 gaccagatca aggagctcag cgtggtgcag gacctcgagg tgatccgcgg cgaggcggtg
     1081 agccgggtgt tcggggcggg cggtgcgcgc gacgcggcgc gcgacgccga cgagcgcctg
     1141 gccctggtcg agggccgcgc cacgatgcgg gtcgaccacg acgccgagca tggcgagcat
     1201 ctgcgcgtgg tgattccctc gctggcctcg cccgcctggc tcggcaagga ctgcatcgcc
     1261 tgccatcagg tgccacaggg cacgccgctc ggcgcggtgt cgatgcgcat ctcgctcgac
     1321 aaggcgaatg ccgcggtgaa tgcgttccgc aaccagagcg cgatgttcgc cgcgctggtg
     1381 tcgatcccgc tgatcctgtt catctacctc ttcatcaccc gcttcgtcac ccgtccgctc
     1441 gacggcctca accggggcct ggggcggatc gccgagggtg gcggcgacct gcgcctgcaa
     1501 ctgccggtgc gcggccgcga cgagatcggc cgcagcgcgc gcaccttcaa cgacatgatg
     1561 ggcacgctcg gcgggctggt gcgccaggtc ggcgactcgg cgagcgcggt gaccgaggcg
     1621 gcgagccggc tggccgccga cgccgaggcg gtcgccgcga gctcgcagcg ccagaacgag
     1681 agctcggtgg gcgcggccgg cgcggtggac gagatggtcg gcagcatctc cgcgatcgcc
     1741 gagcacgccg aggcggtgcg cgagcgctcg cacgacagcc tggaacgctc gcgcgagggc
     1801 cagcgcagcc tggagcgtct ggtcggcgag gtcggcgagg tcgaggccgc ggtgcgccag
     1861 atggccgacg cggtggtcgc cttcgtcgac tccacccagg cgatctcgcg catgaccagc
     1921 gaggtgcgcg agatcgccga gcagaccaac ctgctcgcgc tcaacgctgc gatcgaggcc
     1981 gcgcgcgccg gcgagcaggg ccgcggcttc gcggtggtgg ccgacgaggt gcgcaagctg
     2041 gcggagaaat ccgcgcgctc ggcgggcgag atcgaccaga tcacccgcga ggtggcccgg
     2101 cgttcgagct cggtgcgcgg cgcgctcgac gacggcctcg gtcacctcgc ctccagccgg
     2161 cagatggccg acgcggtgat gcaggtgctg accgcggcga attcgctcgt ggtcgaggtg
     2221 ggcgaaggcc tcgaccgcat cgccggcgcc acgggtgcgc agcgcagtgc gagcgagtcg
     2281 gtcaccgcgc gcatcggcga gatcgccggc atggcgcgca gcaacaacga cgcgctcgcc
     2341 cacaccgtgc gttcggcgcg cgagctcggc gaactcgccc tgcgcctgca ggaatccgtg
     2401 tcgcgcttcc gtttctgagt gcgcccaggc ggacgtggaa agggcgggaa ccgtcgccgg
     2461 tcccgccctc ttgcccgctc gcccttcagc gcgctgccat cactccaggc gtaccggcac
     2521 gaagatgcgt gcctcgccac gctggatcag cagcgcgaag cgcttgccgg cgcgctcgag
     2581 ctgggcgcgc aggtctgcga cgctggcgac cggctggttg ttgatcgcca ggatgacgtc
     2641 gccgcgctgc aggccggcgc gggccgcggc gccgtcggcg ccctccacca ccaccccgcc
     2701 gcgcagctgc aggcgggcgg tctcttccgc ggtgagcgca cgcgcgctca gtccgaactg
     2761 ctcgccgata cgctccacct ggctgggcgc cggtgcgctg ccggccacgg tctcggcctt
     2821 gagctcgtcc agcgtggcgg cgacctcgcg gctgcggccg tcgcgccaca gctccagctt
     2881 cacgcgcgtg cccgggcgct tctcgccgat cacccgcggc agctcggcag agtcgtcgat
     2941 gcggcggcca tcgaccgcga gcaccacgtc gcccgcctgc agcccggcct tgtcggcggc
     3001 gctgcccggc tcgacgctgg cgaccagcgc gccgcgcgga tcggtgaggc cgaaggattg
     3061 ggcgaggtcc ttgtcgacgt tctggatggc gatgccgagg cggccgcgct gcacgcggcc
     3121 gtggctgacg agctggtcct tgatgttcat cgcgacgtcg atcgggatcg cgaacgagat
     3181 ccccatgaag ccgcccgagc gcgagtagat ctgcgagttg atgccgatca cctcgccggc
     3241 gaggttgaac agcggaccgc cggagttgcc cgggttgatc gccacgtcgg tctggatgaa
     3301 gggcacgtag gtctcgtcgg gcaggcggcg cgccttggcc gagatgatgc cggcggtgac
     3361 cgtgttgtcg aagccgaagg gcgagcccat cgccaccacc cattcgccca cgcgggcctg
     3421 gtcggggttg ccgatcttca ccgcgggcag gccgctggcg tcgagcttga gcagggcgac
     3481 gtcggtacga cggtcgatgc cgaccacctt ggccttgaac tcgcgcttgt cgatcagggt
     3541 gacggtgacc tcggagagcg cggcgtcgcc atcctcgccg gcgaccacgt gggcgttggt
     3601 gagcacgtag ccgtccttgc tgacgatgaa gcccgagccg atgccctggc gcgcgcgcgg
     3661 ccccatgccg ggctggccgg ggaagcccgg catgggcacg ccgaagcggc gcaggaagtc
     3721 gtagaagggg tcgttggcga gcgggttctc gcccgccgtg ccctgggccg cgcgctgcac
     3781 cacgcggata ttgaccaccg ccgggcccac ctgctcgacc aggtcgccga agtcgggcag
     3841 gccgaagcgg ttgggcgaga ccatcgacgg ggcggccgcc tgggcgtgcg cggcgctcag
     3901 cgccgaggct tggggaaaga gggtgccgag cgcgagcgcg acggcggtga cggtcaggag
     3961 tttgcgcatg ttgtcgggca tccttgttcg gcaacggaaa gattcggcgc cggagtcccg
     4021 gcggccacct gtcgtggacg cggcctggcg tgcgccgttc ccgacgaaac ctgcgttttc
     4081 aaatcgggtc gggcgcgggc gcttcaagag gctgcgttca aaaagtggtt tcgagcgcga
     4141 gccaggcctg gcggctcacc tcgttggcgg gtcggccggc gaactcgggc tgggcctgca
     4201 gggggcgatt ggcgagttcc tggtggcggc cgagcaggtt gatgccggtc aggcggagct
     4261 ccaccgccat gtcgaacagc cgcaggctgc gcgagacgct ccagtccacc gtggtgtagg
     4321 acggcacggt gtagtcgaag ccgggaacgt agctgaagcc ggcgtcgatc ggccccatgc
     4381 gcagcacgct gagcagcgag cgccacgccc ctgcacgctg cagccagctc aggctggccg
     4441 tgtagggggc gacgttgcgg cggatgcgtg ggtcgtcgac gtcgcggtcg atgaggctgt
     4501 gcgcgaggag gaactcggtg ccgcgccagg gcttcaggtg gagctgggtt tcgatgccgt
     4561 tcagttgtac gcggccgctg tggttctccc agcgcgtgct gcccagccct cgcgagatcg
     4621 cctcggggag cttgccctcg atcggccagt cggagggaac ggcgttgcgc acgatcaggt
     4681 cttcgatgcg ctcgcggaac aggcggacgt cgagcgtgct gcggctgtcg ccgaaaacgc
     4741 cgagaaagcc gatctcgctg gcgtcgatgc gctgggggcg caggtccgga ttggggtatt
     4801 gccggcgctg gaccagctcg cccttgtcgt cggtgatccg tatgtcgacg ttgcgctcga
     4861 aaaggtccgg ctgtcgccat gcgcgcgagt ggccgatgcg ccaggtgatc cgtggagcgg
     4921 cctgccagtt caggaacacg cgtggcgcga gccgcgcctc gtcgccttgc aactgctcca
     4981 gcatgccgcc cgcgttccac aggaaggcgg tgcccgttcg cagctcgaga ttggcgaaca
     5041 ggcggtgttc gtcccgcgca tggttgcggc gctcgtgata ccagaagggc gagtcgtgct
     5101 cgatgcgctt ccattccgcg ccccacatca gccgcgtggc ctcgtccacg cgctcgcgcc
     5161 gctcgatctc caggttgtgc tggacgaagg tggcgctgtt gccgaccgtg ccgcgcaggc
     5221 gctcggggaa ggcgttcgcc agggtccggc tgtttgccgc ggagtcgtag cgccagaggt
     5281 cgtcgcgact ctgccggctc cagtaggccg acacgcgtat cgcgtcgtcc tcggggccgt
     5341 gccgccaccg cagatgcagg gatcggttgg tgtcgcgccc ttcgcgctgg gccgcgatgt
     5401 cgaagatcga gccggcgtat ccgagttcgt gggtgctctc ggcgaccccc gcgttgaggg
     5461 cgagtgcatc gtgttcgccc acctgcaggt cggcgcgaag gttcaggacg tcggtgcggc
     5521 gaccgtcgta caggtcggca aacccttcgt cttcttcatg atgcgcgccg atgcgcacgc
     5581 tgaggggccc gctctgcacg gcgtgtcgca ggctcgcgtc gcggatgccg ggtgcgccgg
     5641 tggcgagctt cagccgggtg ccgggatgtc ggctggcgtc gtcggtgatg atgttgacga
     5701 tgccgaggaa ggcgttggac ccgtaggcgg ccgagttcga gccgcgtacc acctcgatgc
     5761 gttcgatgtc tccgggcagg gcacgctcgg cgctggcggt gaaggatggc gtcagcgacg
     5821 cgcgtccgtc gaccagcagc tggatctgca tcggcgagtc catgccgagg ccgtggtagg
     5881 tcacccactg caggttgccg cgctcctggc cgacctggaa tccgggcacc aggcgcagca
     5941 cgcgcgcgag ctcgcggtag ccggtcgcgg cgatcaggtc cgcatcgatc acggtgaccg
     6001 cgcccgggct gtcctgcaac ggctgcgcca gacgcgaggc gctgagcacc accggcaggg
     6061 gctcgaagaa ggcatctgcg gacgacgctt cgacggtgcc ggcagccagc agcgacagca
     6121 gggcgagagc ggtggggggg cggtggaggt gcatcggaag cgggcggcgg cgcagaatgc
     6181 gcgagtatac ccagtgcgct ttcggggcgc gcagcaacga tcagtcgggg taacatcggg
     6241 caccgtcgac tccgggtgca cgcccttcca tcgtgtcgaa gccatgaccc tgaccgatct
     6301 ccgttatctc gtcgcgctcg cccacgagcg tcatttcggc cgcgccgccg agaagtgcca
     6361 cgtgtcgcag cccacgctgt cggtggcggt gaagaaggtc gaggaggaac tcggcatcca
     6421 gctcttcgag cgcagcgcct cggaggtcaa gatcaccgag accgggcgcc ggatcgtcgc
     6481 ccaggccgag cgggtgctga tggaggcgag ccagatccag gagatcgccg ccgcaggcaa
     6541 ggacccgctc gccgggccgc tgcgtgtggg cgtgatctac accatcggac cctacctgct
     6601 gccgcggctg attccgcgcg tgcatcagct cgcgccccgg atgccgctga tcatccagga
     6661 gaacttcacc gcgcgtctgg ccgaggcgct caagcgcggc gaactcgacg tgatcatcat
     6721 cagcctgccc ttcgacgagg cgggcgtggt ggcgcagccc gtgtatgacg agcccttccg
     6781 cgtgctgatg ccggccgacc atccgtggaa tgcggaaagc gagatcgacc cccagcatct
     6841 cgccgacgac cagctcctgc tgctcggtgc cggcaactgc ttccgcgacc aggtgctgga
     6901 ggtgtgcccg agctgccgca acatcggcgg gctgcagcgc acgctcgagg gcagctcgct
     6961 cgagaccatc cgccacatgg tggccaccgg cctcggcgtg accgtgctgc cgagctcggc
     7021 agcggacgag atggcgacgc agaatccgct ggtcgcggtg cggcctttca ccatgccgga
     7081 gccctcgcgt cgcgtcgcgc tcgcctggcg ggtgacctat ccgcgcaacg gggcgatcga
     7141 cgtgctgcgc gcggcgatcc tcgacagcgc cctgccgggc gtgcgtccgg tgggccgcgc
     7201 cgtgccgccc gggcaggtgc cgtgggtgga ttcgcgatga aaattcttga tcggatccat
     7261 cagggtaatc ggtttgatca atgcatccct caggcgctaa catgaagcgc aaacccctgc
     7321 gggcggtgtg aagcctgcat acacaggaga accagcatgg aaatcgacat cggcatcaag
     7381 gaaaaggacc gcgcgaagat cgcggagggc ctgtcgcgtc tgctcgccga cagctacacg
     7441 ctttacctga ccacgcacaa cttccactgg aacgtgaccg ggccgatgtt caacaccctg
     7501 catctgatgt tcgagacgca gtacaacgaa ctcgcgctcg cggtggacga gatcgccgaa
     7561 cgcatccgtg cgctcggctt cccggcgccg ggcacctaca aggaattcca gaagctcacc
     7621 agcatggccg agccggaagg tgtgccgaac gcgcaggaga tgatccgcct gctggtgcag
     7681 ggtcaggaag cggttgtcaa gaccgcccgc agcatcgccc cgatcgccga caaggcgagc
     7741 gacgagccca cgctcgacct gctcacccag cgcatgcagg tgcacgagaa gaccgcctgg
     7801 atgctgcgca gcctgctcga aggctgattc ccgccgcgct tgtgcagcaa atgaacgggc
     7861 ccgcggggcc cgttcctgta tctgtggcga ctcgcgtcgc tcctacggac g
//
LOCUS       DAOQBS010000134         7881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_8820_length_7881_cov_16.822387, whole genome shotgun sequence.
ACCESSION   DAOQBS010000134 DAOQBS010000000
VERSION     DAOQBS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8820_length_7881_cov_16.822387"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PKN25_12770"
     CDS             complement(<1..663)
                     /locus_tag="PKN25_12770"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNV91818.1"
                     /translation="MTVATLLVELLTEELPPKALPRLGETFATRIAEGLKAKGLAPVD
                     AAFRSFASPRRLAVTVAQVAAEAAAKEVTEKLMPVSVALDAEGKPTPALLKKMEAKGI
                     AAEAVARFERRLDGKAEALFHTAMVPGAKLADVLSGVVQDAIKALPIPKVMRWGDGDA
                     TFVRPVHKLTLLHGAEVVPGRVLDLDSGRTSRGHRFMSRGDLSIATADAYEPTLLAEG
                     KVI"
     gene            complement(663..1589)
                     /gene="glyQ"
                     /locus_tag="PKN25_12775"
     CDS             complement(663..1589)
                     /gene="glyQ"
                     /locus_tag="PKN25_12775"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNV91819.1"
                     /translation="MTTESRLVKPTFQQVILTLQHFWGERGCVLLQPYDLEVGAGTSH
                     TATFLRAIGPEPWNAAYVQPSRRPKDGRYGENPNRLQHYYQFQVVLKPSPLNIQELYL
                     DSLRALGIDPTVHDIRFVEDDWENPTLGAWGLGWEVWLDGMEVTQFTYFQQVGGLDCK
                     PVLGEITYGIERLAMYLQGVENVYDLVWSVAPDGRKVTYGDVYHQNEVEQSTFNFEHS
                     NVDFLFSLFNNYESEAKRVMEVGLALPAYEMVLKAAHTFNLLDARGAISVTERAAYIG
                     RIRNLSRAVAQAYFESREALGFPMLNAEKEAA"
     gene            complement(1638..3167)
                     /gene="lnt"
                     /locus_tag="PKN25_12780"
     CDS             complement(1638..3167)
                     /gene="lnt"
                     /locus_tag="PKN25_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNV91820.1"
                     /translation="MRRAWLLALLAGGATVFAFAPFGLFPLAFLGLGVLVWLLAGAAR
                     AREGFALGFAWGFGAFAAGVSWLYVALERYGGVPAPVAALAIALFCAYLALYPALAGA
                     AFVALRGERVAGAPVRAAALFGALWLGSEWLRGVLFTGFPWLAIGYAQTPPSPLAGLL
                     PVLGVYGVGGLSAFVAALCAFALRAGGVRVLLAWRPALALCAPFAAGFALLGHAWTQP
                     VGAPLKVALVQTDFAQSLKWDPARFEEVLRVNLELARDAFGQPQPPALVVLPETTVPT
                     LLDRLPAGYLEAFAGLAAASGADLVMGAFRRDAEERIYNAAISLGSAPVQLYAKQHLV
                     PFGEYSPPLFGWFYALAQIPMSDQTRGAPDQAPMRFGAQQVALNICYEDLFGAELIRA
                     LPAATLMLNLSNLAWYGDSLAQPQHLQIARVRALETGRPMLRATNTGMTAVVQPDGSV
                     AAVLPAFARGVLHAEVRGHQGMTPYARWGDRLALLAAAIPIVLAFLAAAAARPRRTEA
                     R"
     gene            complement(3167..4006)
                     /locus_tag="PKN25_12785"
     CDS             complement(3167..4006)
                     /locus_tag="PKN25_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNV91821.1"
                     /translation="MDSSPKPSLLERLSALLSREPEDRDELLALLHSAFDRNLIDADA
                     LTIIEGALQMSDMQVRDVMVPRAQMDCVHLDDPIDTIANFAIDTAHSRFPAIGDGKDD
                     VVGILLAKDLLRYFAGREFDLRDMLRPAVFVPESKRLNVLLREFRVSRNHMAIVVDEY
                     GGVAGLVTIEDVLEQIVGDIEDEYDFDEVGDNIRLDHSGRYRVKATTEIEDFNEAFAT
                     HFSDEEYDTVGGLVIRHFGRLPKRGEAVELEGLKIQVLRADSRRVYTLIVERLPQPLS
                     EQA"
     gene            complement(4029..4853)
                     /gene="ybeY"
                     /locus_tag="PKN25_12790"
     CDS             complement(4029..4853)
                     /gene="ybeY"
                     /locus_tag="PKN25_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNV91822.1"
                     /translation="MANKTNRTDRTNQAAARKSAGTWPRIIAVDADGKAARIRAERLE
                     IELGDGRKLLLAFPEKAWGDLEIEAETDDDNAVPMLSLQPGACNLMTLRVDVHHDMSF
                     VEPLALPAGEHPPVLKLQVQKAVEGEDKANCPKKNHIRRWAAAALRASAQVTVRLVGE
                     AEGRALNRDYRGKDYATNVLTFAYAEGEDLPGLPAAAADVPLAGDLVLCVPVVVREAA
                     AQGKTLEAHFAHLVVHGMLHLQGYDHENETEAAEMEALETAILRELGYADPYALSA"
     gene            complement(4843..5862)
                     /locus_tag="PKN25_12795"
     CDS             complement(4843..5862)
                     /locus_tag="PKN25_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNV91823.1"
                     /translation="MAKILEVFFEPVDNARLAKLCGVLDENLRQIENAFDITVSRRAE
                     HFTLQGHPAQVLRGEMALKHFYALADKDLSRDEVQLGLIEIANKGEAAQPAPVLMTRR
                     TELHGRTPRQVDYLRNIQDFDITFGIGPAGTGKTYLAVASAVDAFERDLVERIILTRP
                     AVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFDRVGKLFERGSIEIAPLAFMRG
                     RTLNNAFIILDEAQNTTPEQMKMFLTRIGFGAKAVVTGDLTQIDLARGQRSGLKEARA
                     VLAGVRGIAFTEFSKEDVVRHPLVARIVEAYDLEAARLEREKAAARAARQQPHPQEQE
                     AEDGE"
     gene            complement(5863..>6710)
                     /locus_tag="PKN25_12800"
     CDS             complement(5863..>6710)
                     /locus_tag="PKN25_12800"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNV91824.1"
                     /translation="SRPLEDVLAEIAGLAAQGVKEVTLLGQNVNAWRGVLSREDGEQG
                     ADVGDFAFLLECVAEIPGIERIRYTTSHPREMTQRLIDCYAKLPKLVSQLHLPVQSGS
                     DRVLAAMKRGYSVLEFKSVVRKLRAARPDLSLTSDFIVGFPGETEEDFEKTMKLIDDV
                     GFDGSFSFVYSSRPGTPAADLEDPVAQETKLAWLARLQKRIDEQYQANSEAMVGTVQR
                     ILVEGAAKKNAEAEMMGRSDNNRVVNFPSDSPVRDRLIGQFVEVRITAALPHSLRGEI
                     LTRES"
     assembly_gap    6711..6810
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6811..7382)
                     /locus_tag="PKN25_12805"
     CDS             complement(<6811..7382)
                     /locus_tag="PKN25_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336071.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNV91825.1"
                     /translation="MKKLYIRTFGCQMNEYDSDKMADVLGAHEELVKTDNPEEADVIL
                     FNTCSVREKAQERVFHDLGRVRLLKQKKPGLIIGVGGCVASQEGEAIVKRAPYVDVVF
                     GPQTLHRLPSLIAARKQSGRSQVDISFPEIEKFDAMPPARVEGASAFVSIMEGCSKYC
                     TFCVVPYTRGDEVSRPLEDVLAEIAGLAAQG"
     gene            complement(7441..>7881)
                     /locus_tag="PKN25_12810"
     CDS             complement(7441..>7881)
                     /locus_tag="PKN25_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91826.1"
                     /translation="VERLIYKAFALTSPGRWTIIGALFPTVPDLSGPSLRPHFLSVLL
                     AGLSGLASSVAFAALPRPIPDDAARVKMTFSSASVVQVKDATLQLSPGAQIRDTHNRI
                     VLPSHVSGEYVVRMLVDRNGQVHRVWILTPEEAAAPMPKAPSRP"
BASE COUNT         1213 a         2549 c         2807 g         1212 t
ORIGIN      
        1 gatgaccttg ccctcggcga gcagcgtggg ctcgtaggcg tcggcggtgg cgatggagag
       61 gtcgccgcgg ctcatgaagc ggtggccgcg ggaggtacgg ccggaatcca gatccagcac
      121 gcggcccggc accacctcgg cgccgtgcag cagggtgagc ttgtgcaccg ggcgcacgaa
      181 ggtggcgtcg ccatcgcccc agcgcatgac cttggggatg ggcagcgcct tgatggcgtc
      241 ctgcacgacc cccgacagca cgtcggcgag cttcgcgccc ggcaccatgg cggtgtggaa
      301 caaggcctcg gccttcccgt ccaggcggcg ctcgaagcgg gcgacggcct cggcggcgat
      361 gcccttcgct tccatcttct tcagcaacgc cggggtgggc ttgccctcgg cgtcgagcgc
      421 cacggacacg ggcatcagct tttcggtgac ctccttcgcg gcggcctcgg cagccacctg
      481 cgccacggtc accgccagcc ggcgcggact ggcgaaactg cggaaggcgg cgtcgacagg
      541 cgccagtccc ttggctttca ggccctcggc gatcctggtg gcgaaggtct cgcccaggcg
      601 cggcagggcc ttgggcggca gttcttcggt gagcagttcg acgagcaggg tcgcaacggt
      661 catcacgcgg cctccttttc agcattcagc atcgggaagc cgagggcttc gcgggattcg
      721 aaataggcct gggcgaccgc gcgcgagagg ttgcggatgc ggccgatgta ggcggcgcgc
      781 tcggtcaccg agatcgcgcc gcgggcgtcg agcaggttga aggtgtgggc ggccttgagc
      841 accatctcgt aggccggcag tgccagcccg acctccatca cgcgcttggc ctcggactcg
      901 tagttgttga atagcgagaa caggaagtcg acgttgctgt gctcgaagtt gaacgtcgat
      961 tgctcgacct cgttctggtg gtaaacgtcg ccgtaggtga ccttgcgacc gtccggcgcg
     1021 acagaccaca ctaggtcata gacgttctcc acgccctgca ggtacatcgc caggcgctcg
     1081 atgccgtagg tgatctcgcc cagcaccggc ttgcaatcga ggccgccgac ctgctggaag
     1141 taggtgaact gggtgacctc catgccgtcc agccagactt cccagcccag tccccaggcg
     1201 cccagggtgg ggttctccca gtcgtcctcg acgaagcgga tgtcgtgcac cgtcgggtcg
     1261 atgccgagcg cgcgcaggct gtcgaggtag agctcctgga tgttcagcgg cgagggcttc
     1321 agcaccacct ggaactggta gtagtgctgc aggcggttgg ggttctcgcc gtagcggccg
     1381 tccttggggc ggcgcgaggg ctgcacgtag gcggcgttcc agggctcggg gccgatcgca
     1441 cgcaggaagg tggcggtgtg gctggtgccg gcgcccacct cgaggtcgta gggctgcagc
     1501 agcacgcagc cgcgctcgcc ccagaagtgc tggagcgtga ggatgacttg ctggaaggtc
     1561 ggtttcacga gtcgggattc ggtggtcatg gtctcgggca gcctccgggc tgcgcagcgc
     1621 acgcaaaaac gatgatttta ccgggcttcg gtccggcgcg ggcgtgccgc ggcggcggcg
     1681 aggaatgcga ggacgatcgg gatcgcggcg gcgaggagcg cgaggcgatc gccccagcgc
     1741 gcataaggag tcatgccctg gtggccgcgc acctcggcat ggagcacgcc gcgcgcgaac
     1801 gcgggcagca cggcggcgac gctgccgtcg ggctgcacca ccgcggtcat gccggtgttg
     1861 gtggcgcgca gcatcggacg accggtctcg agcgcgcgca cgcgggcgat ctgcaggtgt
     1921 tgcggctggg cgagcgagtc gccgtaccag gccaggttgg agaggttgag catcagcgtg
     1981 gccgcaggca gcgcgcggat cagctcggcg ccgaagaggt cctcgtagca gatgttgagc
     2041 gcgacctgct gcgcgccgaa gcgcatcggc gcctggtcgg gcgcgccgcg ggtctggtcg
     2101 gacatcggaa tctgcgccag cgcgtagaac cagccgaaca gcggcggtga atattcgccg
     2161 aagggcacca ggtgctgctt ggcgtagagc tgcacgggcg cgctgccgag gctgatcgcg
     2221 gcgttgtaga tgcgctcctc ggcgtcgcgg cggaaggcgc ccatcaccag gtcggcgccg
     2281 gacgcggcgg cgaggccggc gaaggcttcg agatagccag cgggcaggcg gtcgagcagg
     2341 gtcggcacgg tggtctcggg cagcaccacc agcgcaggcg gctgcggctg cccgaaggcg
     2401 tcgcgcgcga gctcgaggtt gacccgcagc acctcctcga agcgcgccgg atcccatttg
     2461 aggctctgcg cgaaatcggt ctgcaccagc gccaccttca agggcgcgcc gaccggctgg
     2521 gtccaggcat ggccgagcag cgcgaagccg gcggcgaagg gagcgcacag cgccaacgcc
     2581 gggcgccagg cgagcagcac gcgcacgccg cccgcccgca gcgcgaatgc gcacagcgcg
     2641 gcgacgaagg cgctcagccc ccccacgcca tacacgccga gcaccggcag caggccggca
     2701 agcgggctgg gcggcgtctg cgcatagccg atggcaagcc aggggaagcc ggtgaagagc
     2761 acgccgcgca gccattcgga gcccagccag agcgcgccga agagcgccgc tgcgcgcacc
     2821 ggcgcgcctg ccacgcgctc gccgcgcagc gcgacgaagg ccgccccggc gagcgccgga
     2881 tagagcgcga ggtaggcgca gaacagcgcg atcgccagcg cggcgaccgg cgcgggcacg
     2941 ccgccgtagc gttcgagcgc gacgtagagc caggacactc cggcggcgaa ggcaccgaag
     3001 ccccacgcga agccgagcgc gaagccctcg cgcgcgcgcg ccgcgccggc gagcagccag
     3061 acgagcacgc cgaggccgag gaaagcgagc gggaacagcc cgaagggcgc gaaggcgaac
     3121 accgtcgcgc cgccggcgag cagcgcgagc agccaggcgc gccgcatcac gcctgttcgg
     3181 acagcggctg cggcaggcgc tcgacgatca gcgtgtacac gcgccggctg tcggcgcgca
     3241 gcacctggat cttcaggcct tcgagctcca ccgcctcgcc acgcttgggc aggcggccga
     3301 aatggcggat caccaggcca ccgacggtgt cgtattcctc gtcgctgaag tgggtggcga
     3361 aggcctcgtt gaagtcctcg atctcggtgg tcgccttcac gcggtagcgg ccgctgtggt
     3421 cgagacggat gttgtcgccg acctcgtcga agtcgtactc gtcctcgatg tcgccgacga
     3481 tctgctcgag cacgtcctcg atggtgacca ggccggccac gccgccgtac tcgtcgacca
     3541 cgatcgccat gtggttgcgc gacacgcgga actcgcgcag cagcacgttg agacgcttgg
     3601 attcgggcac gaagactgcg gggcgcagca tgtcgcgcag gtcgaactcg cgcccggcga
     3661 agtagcgcag caggtccttg gcgagcagga tgccgacgac gtcgtccttg ccgtcgccga
     3721 tcgcggggaa gcgcgagtgg gcggtgtcga tcgcgaagtt ggcgatcgtg tcgatcgggt
     3781 cgtccagatg cacgcagtcc atctgggcgc gcgggaccat cacgtcgcgc acctgcatgt
     3841 cggacatctg cagggcgcct tcgatgatgg tgagggcgtc ggcgtcgatc aggttgcggt
     3901 cgaaggccga gtgcagcagc gcgaggagtt catcgcgatc ttccggttcg cgcgagagaa
     3961 gggccgaaag tcgctcgagt aaggagggtt tgggggaact gtccattttg ggggtcggtg
     4021 tctccgcttc aggcgctcaa ggcgtagggg tcggcatagc cgagctcgcg caggatcgcg
     4081 gtctcgagcg cttccatctc ggccgcctcg gtctcgttct cgtgatcgta accctgcagg
     4141 tgcagcatgc catgcacgac gaggtgggcg aagtgcgcct ccagggtctt gccctgggcg
     4201 gcggcctcgc gcaccaccac cggaacgcac agcaccaggt cgccggcgag cggcacgtct
     4261 gcggccgccg cgggcaggcc gggcaggtcc tcgccctcgg cgtaggcgaa ggtcagcacg
     4321 ttggtggcgt agtccttgcc gcggtagtcg cggttgagtg cgcgcccctc ggcctcgccc
     4381 accaggcgca ccgtgacctg ggcgctggcg cgcagcgccg ccgcggccca gcgccggatg
     4441 tggttcttct tggggcagtt ggccttgtcc tcgccctcga ccgccttctg cacctgcagc
     4501 ttcagcaccg gcggatgctc gcccgcgggc agcgcgagcg gctcgacgaa gctcatgtcg
     4561 tggtgcacat ccacgcgcag ggtcatcagg ttgcaggcac cgggctgcag cgacagcatc
     4621 ggcaccgcgt tgtcgtcgtc ggtctcggcc tcgatctcga ggtcgcccca ggccttctcg
     4681 gggaaggcga gcagcagctt gcgaccgtcg ccgagctcga tctccaggcg ctcggcgcgg
     4741 atgcgcgcag ccttgccgtc ggcatcgacg gcgatgatcc tgggccaggt gccggccgac
     4801 ttgcgcgcgg ccgcctggtt cgtcctgtcg gtcctgttgg tcttattcgc catcttcggc
     4861 ttcctgctcc tgcgggtgcg gctgctggcg cgcggcgcgg gccgcggcct tctcgcgctc
     4921 gaggcgcgca gcctcgaggt cgtaagcttc gacgatgcgc gcgacaagcg gatgacgcac
     4981 cacgtcttcc ttgctgaatt cggtgaacgc gatgccgcgt acccccgcca gcaccgcgcg
     5041 cgcctccttg agaccgctgc gctggccgcg tgccaggtcg atctgggtga ggtcgccggt
     5101 gaccaccgcc ttggcgccga agccgatgcg ggtgaggaac atcttcatct gctcgggggt
     5161 cgtgttctgc gcctcgtcga ggatgatgaa ggcattgttg agcgtgcgcc cgcgcatgaa
     5221 ggcgagcggc gcgatctcga tgctgccgcg ctcgaagagc ttgccgacgc ggtcgaagcc
     5281 catcaggtcg tagagcgcgt cgtagagcgg gcgcaggtag gggtcgacct tctgcgccag
     5341 gtcgccgggc aggaagccca ggcgctcgcc ggcctcgacc gccgggcggg tgaggatgat
     5401 gcgctcgacg aggtcgcgct cgaaggcgtc gaccgcgctc gccaccgcga gataggtctt
     5461 gcccgtgccg gccgggccga tgccgaaggt gatgtcgaag tcctggatgt tgcgcaggta
     5521 gtcgacctgg cgcggcgtgc ggccgtggag ctccgtacgg cgcgtcatca gcaccggcgc
     5581 gggctgcgcc gcctcgccct tgttggcgat ctcgatgagg ccgagctgga cctcgtcgcg
     5641 cgacaggtcc ttgtcggcga gcgcgtaaaa atgcttgagc gccatctcgc cgcgcagcac
     5701 ctgcgcgggg tggccctgca gcgtgaagtg ttcggcacgc cggctcacgg tgatgtcgaa
     5761 ggcgttctcg atctggcgca ggttctcgtc gagcacgccg cacagcttgg ccagccgggc
     5821 gttatccacc ggctcgaaga agacttccag gattttcgcc attcaggatt cccgggtgag
     5881 gatttcgccg cgcaggctgt gcggcaatgc tgcggtgatg cgcacctcga cgaactggcc
     5941 gatgaggcgg tcgcggacgg gcgagtcgga ggggaagttg accacgcggt tgttgtcgct
     6001 gcggcccatc atctcggcct cggcgttctt cttcgccgcg ccctcgacca ggatgcgctg
     6061 caccgtgccg accatggcct cgctgttggc ctggtactgc tcgtcgatgc gcttttgcag
     6121 gcgggcgagc caggcgagct tggtctcctg cgccaccggg tcttcgaggt cggcggccgg
     6181 ggtgccggga cgcgagctgt agacgaagct gaaggagccg tcgaagccga cgtcgtcgat
     6241 cagcttcatg gtcttctcga agtcctcctc ggtctcgccg gggaagccga cgatgaagtc
     6301 cgaggtcagg gaaagatccg gccgcgccgc acgcagcttg cgcaccacgg acttgaactc
     6361 gagcaccgag tagccgcgct tcatcgccgc cagcacgcgg tccgagcccg actgcaccgg
     6421 caggtgcaac tgcgacacca gcttgggaag cttcgcgtag cagtcgatca gacgctgcgt
     6481 catctcgcgt gggtgcgagg tggtgtagcg gatacgctcg atgccgggga tctcggccac
     6541 gcattccaga aggaaggcga aatcaccaac gtcggcgccc tgctcgccgt cctcgcgcga
     6601 gagcacgcca cgccaggcgt tgacgttctg gccgagcagc gtcacctcct tgacgccctg
     6661 tgccgcgaga ccggcgatct cggcgagcac gtcctcgagc gggcgcgaca nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccctgtgccg cgagaccggc gatctcggcg
     6841 agcacgtcct cgagcgggcg cgacacctcg tcaccgcggg tatagggcac gacgcagaag
     6901 gtgcagtact tcgagcagcc ttccatgatc gacacgaagg cgctcgcgcc ctcgacgcgc
     6961 gccggcggca tggcgtcgaa cttctcgatc tcggggaagg agatatccac ctgcgagcgc
     7021 ccgctctgct tgcgcgccgc gatcaggctc ggcaggcggt gcagggtctg cgggccgaac
     7081 accacgtcca catagggcgc gcgcttgacg atggcctcgc cttcctggct ggccacgcag
     7141 ccgcccacac cgatgatcag cccgggcttc ttctgcttga gcagacgcac gcggccgaga
     7201 tcgtggaaca cccgctcctg cgccttctcg cgcaccgaac aggtgttgaa gaggatcacg
     7261 tcggcctctt ccgggttgtc ggtcttgacc agttcttcgt gagcgccgag cacgtccgcc
     7321 atcttgtcgg agtcgtactc gttcatctgg cacccgaagg tgcggatgta gagtttcttc
     7381 atctatcagg gaatgtcagg caaggccttg cggcaggtcg ggcgggcatc gcggcatcca
     7441 tcaaggccgg gacggcgcct tgggcatcgg cgccgcagcc tcttccgggg tgaggatcca
     7501 cacccgatgc acctggccgt tgcggtcgac cagcatgcgc accacgtact cgccagacac
     7561 atgcgagggc agcacgatgc ggttgtgggt atcgcggatc tgcgcgccgg ggctgagttg
     7621 cagcgtggca tccttgacct gcaccacgct ggccgaggaa aacgtcatct tcacccgcgc
     7681 cgcgtcgtcg ggaatcggcc gcggcaaggc agcaaaggcc acggaggacg cgaggccgga
     7741 gagaccggcc agcaggacgg agaggaaatg cgggcgcaag gacgggccgg aaagatccgg
     7801 aacggtaggg aacagggcgc cgatgatcgt ccatcgcccc ggggatgtca atgcaaaagc
     7861 cttataaatc aagcgttcca c
//
LOCUS       DAOQBS010000135         7870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_8839_length_7870_cov_15.467051, whole genome shotgun sequence.
ACCESSION   DAOQBS010000135 DAOQBS010000000
VERSION     DAOQBS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8839_length_7870_cov_15.467051"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKN25_12815"
     CDS             <1..969
                     /locus_tag="PKN25_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNV91827.1"
                     /translation="DEIEAKVLKFDQEKNRVSLGLKQLGEDPWVGISRRYPQGTRLFG
                     KVTNITDYGAFVEVEQGIEGLVHVSEMDWTNKNIHPTKVVQLGDEVEVMILEIDEDRR
                     RISLGMKQCMSNPWDDFAINHKKGDKVRGQIKSITDFGVFIGLEGGIDGLVHLSDLSW
                     SESGEDAVRRFKKGDEVEAVVLAIDVERERISLGIKQLEGDPYTNFIATHEKNSLVRG
                     TVKSVEARGAVISLGDDVEGYLRASEAAPHRVDDLTTMFKEGDEVEALVINVDRKTRS
                     ISLSIRAKDQVEQSEAMSKLASESSASAGTTNLGALLKAKLNEQKQ"
     gene            981..1268
                     /locus_tag="PKN25_12820"
     CDS             981..1268
                     /locus_tag="PKN25_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HNV91828.1"
                     /translation="MTKSELIAQLAERFPQLVAKDADYAVKMILDAMTDALARGDRIE
                     IRGFGSFALNYRPPRTGRNPKSGEKVLVPEKYVPHFKAGKELRERVDLGGS"
     gene            1354..1662
                     /locus_tag="PKN25_12825"
     CDS             1354..1662
                     /locus_tag="PKN25_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNV91829.1"
                     /translation="MRAIIWIIRLLLFFLLFGFAIKNDHLVTLNFFFGIQWQLPLVFV
                     ILVTFAAGALIGVTATLASMMRQRREISRLRRQVELVEREKASTETALAAAESRLPMP
                     "
     gene            1669..2844
                     /gene="lapB"
                     /locus_tag="PKN25_12830"
     CDS             1669..2844
                     /gene="lapB"
                     /locus_tag="PKN25_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HNV91830.1"
                     /translation="MEIEYWWLLALPLFFALGWLAARIDIRQVVQESRALPRSYLSGL
                     NFLLNEQPDKAIDAFVEAVRIDPQTVELHFALGSLFRRRGETDRAIRIHQLLVDREDI
                     SDEHRLQALGELGQDFLKAGLLDRAEAAFLRLRGTRANDVALRYLLEIYQQEKDWAKA
                     IEVAEALPGHEGVMWHTEVANFHCELAATALANSRHDEARGHLDRAFEVNRRCVRASL
                     LLGDLHAAQGRDEEALEAWQRIENQDPNYLALVAERVMDACGRLGRVAQGHQLLRAWL
                     AGHASLDLLDELFHWELEREGPKAAYEMVREELRRNPTLLGLDKLLEAAALNAPAEQR
                     ADIDLIKQLIHGHTRRVARYRCNTCGFKARQFHWRCPACGGWETYPPRRTEEFDLTP"
     gene            2925..3815
                     /gene="cysM"
                     /locus_tag="PKN25_12835"
     CDS             2925..3815
                     /gene="cysM"
                     /locus_tag="PKN25_12835"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HNV91831.1"
                     /translation="MEFRTLEDYVGRTPLVRLKRIDAGRNNVILAKLEGNNPAGSVKD
                     RPALSMVMRAEARGQIKPGDTLIEATSGNTGIALAMAAAIRGYRMILVMPENQSVERR
                     QTMRAFGAELVLVPSSGGMEMARDIAEKMRDEGRGIILDQFANPDNPVAHYEGTGPEL
                     WEQTGGRITHFVSSMGTTGTIMGTSRFLKERNPDICIVGCEPEEGSSIPGIRKWPEAY
                     LPKIFERPRVDRFERVSQADAEEMTRRLAREEGIFAGISSGGAMHVALRIAAQVENAV
                     IVSIVCDRGDRYLSTGVFPA"
     gene            3988..5997
                     /locus_tag="PKN25_12840"
     CDS             3988..5997
                     /locus_tag="PKN25_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91832.1"
                     /translation="MLALQIAEITRPGLAAEELSVRFDAHAQSGEIAVGRLRVGAREW
                     RALSLRCGRLHLDDGVLGCSAARLELRGRRLPFEADIEATLGPGPARIVLRLAEGGRI
                     EAAIQADGSLRARLHRIRPAATAALLEPWLAELAARLRELEAVGVLDAELDYRPAGVG
                     DASATLRGRIAGGGFGSGDGLRAAEGVEAGFTLDARGTGAAWSWAAQLDWDAGAAYVH
                     PLLFEAGPRLRAHGRFDGRRIVLERGELALDGVAAASARGVFDTFAGTLDTLAVELTD
                     VDLVRIGPRWLAPLLAPASAQRLRLEGRADLRAELRRDRLETVDVELHGAGFGLASPQ
                     GEGIAAGGVAEEARAEVLAFGPVDGRVRWSSRGRGEAALQVGGGRWEKLALGAFALDA
                     VLGPAGLRLPGARIPLLDGALVLRDLALGWSVAGWEGSAGAVLEPVSMPLLTEALGLP
                     RMAGVMSAALPGLRLRPGELVLDGTLAVSVFGGWVQASGLRVHEPFGVASHLTGEIEA
                     RHIDLAQLTEAFSFGSITGHIDADVRGLELSSWRPTAFDARIASIAGDERRRISQRAV
                     QNLGALGGGGALAAVQRSVLRLFETFGYRELGLRCRLAKGVCEMDGLDAAPDGAARAD
                     GGFTIVRGGGVPALDVIGYNRRVDWNELVARLQRVVAEGVSPTID"
     gene            6022..6216
                     /locus_tag="PKN25_12845"
     CDS             6022..6216
                     /locus_tag="PKN25_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91833.1"
                     /translation="MNPVARWLFAAALVASTAACVTINIYFPAAAAEKAADRIIDEVW
                     QLREGAGSAPVTPPAAGDAK"
     gene            6213..6653
                     /locus_tag="PKN25_12850"
     CDS             6213..6653
                     /locus_tag="PKN25_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HNV91834.1"
                     /translation="MKSASMPSGSWAGRLAALFVGLALAFPVHAQGNLEIDTPAISAL
                     RKTMQQRHAQLAPLYASGAVGIAADGTVSLRDAAAVPLAQRAQANAAVAAENADRAAL
                     YREIARANGHPEWEAEVRRTFAQRWLERAQPGWWVQQGAAWSRK"
     gene            6650..7441
                     /locus_tag="PKN25_12855"
     CDS             6650..7441
                     /locus_tag="PKN25_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNV91835.1"
                     /translation="MMPVLVFDIETIPDVAGIRAIENLPAGLSDYEVAEWAFQQRRAS
                     HGNDFLPHHLQRVVAISCVLRDAQHFRVFSLSEPESGEGEIIQRFFDGIERYSPQIVS
                     WNGGGFDLPVLHYRGMLHGVAAPRYWDQGEGDYHDSRDFKWNNYISRYHSRHLDLMDL
                     LALYQPRASAPLDDLAKLMGYPGKLGMDGSAVWQAWQEGRIAEIRDYCETDVVNTYLV
                     YLRFERMRGHLDAAAWAAEVETVREALGRIDAPHWKQFLAAWPQG"
     gene            7488..7730
                     /locus_tag="PKN25_12860"
     CDS             7488..7730
                     /locus_tag="PKN25_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNV91836.1"
                     /translation="MGIIGTILIGLIVGFVARLLHPGKDGLGIIMTSLLGIAGSLVAT
                     YGGQALGIYHAGEGAGFLGAVIGAVVILFVVTRLKK"
BASE COUNT         1176 a         2692 c         2742 g         1260 t
ORIGIN      
        1 gacgagatcg aagccaaggt gctcaagttc gaccaggaaa agaaccgcgt ctcgctcggc
       61 ctcaagcagc tcggcgaaga cccgtgggtg ggcatctcgc gccgctaccc gcagggcacc
      121 cgcctgttcg gcaaggtcac caacatcacc gactacggcg ccttcgtcga ggtcgagcag
      181 ggcatcgagg gcctggtcca cgtgtccgag atggactgga ccaacaagaa catccacccg
      241 accaaggtcg tgcagctggg cgacgaggtc gaggtcatga tcctcgagat cgacgaagac
      301 cgtcgccgca tctcgctggg catgaagcag tgcatgtcca acccgtggga cgatttcgcc
      361 atcaaccaca agaagggcga caaggtccgt ggccagatca agtcgatcac cgacttcggc
      421 gtgttcatcg gcctggaagg cggcatcgac ggcctggtgc acctgtccga cctgtcgtgg
      481 agcgagtccg gcgaggatgc cgtgcgccgc ttcaagaagg gcgacgaggt cgaggccgtg
      541 gtgctggcga tcgacgtcga gcgcgagcgc atctcgctcg gcatcaagca gctcgagggc
      601 gacccctaca ccaacttcat cgccacccac gagaagaaca gcctcgtgcg cggcaccgtg
      661 aagtcggtcg aggcccgtgg cgcggtgatc tcgctgggtg acgacgtcga aggctacctg
      721 cgcgcgtccg aggccgctcc gcaccgtgtc gacgacctca ccaccatgtt caaggaaggg
      781 gacgaggtcg aggcgctggt gatcaacgtg gatcgcaaga cccgctcgat cagcctgtcg
      841 attcgtgcca aggaccaggt cgagcagtcc gaagccatga gcaagctggc ttccgagagc
      901 tccgcttccg ccggtaccac caacctgggt gccctgctca aggccaagct gaacgagcag
      961 aagcagtaat ctgtccgatc atgaccaaat cggagctgat cgcccagctg gcggagcgtt
     1021 ttccccagct ggtcgcaaag gacgccgatt acgcggtcaa gatgattctc gatgcgatga
     1081 ccgacgcgct cgcgcgcggt gatcgcatcg agatccgcgg gtttggcagc tttgcgctga
     1141 actaccgtcc gccccgtacc gggcgcaatc ccaagtccgg cgagaaggtg ctggtgccgg
     1201 aaaaatacgt cccgcatttc aaggcgggca aggaattgcg cgaaagggtg gatctcggcg
     1261 gaagttgacc gcgcggcgat cggtttacag gcaataatgg agggcgactg cggtcgcctt
     1321 ctttttgttc atcgttcggg aatcatgccg gtcatgcgtg caatcatctg gatcatccgc
     1381 ctgctgctgt tcttcctctt gttcggcttc gcgatcaaga acgaccatct ggtcacgctg
     1441 aacttctttt tcggcatcca gtggcaactt ccgctggtgt tcgtgatcct ggtcaccttc
     1501 gccgccggcg ccctgatcgg cgtcaccgcg acgcttgcct cgatgatgcg ccagcgccgc
     1561 gagatctcgc gcctgcgccg ccaggtcgag ctcgtcgagc gcgagaaggc ctcgaccgag
     1621 accgcgctcg ccgcggcaga gtcccgcttg ccgatgccct gatccccgat ggaaatcgaa
     1681 tactggtggc tgctcgcgct gccgctgttc tttgcgctcg gctggctggc cgcgcgcatc
     1741 gacatccgcc aggtggtgca ggagtcgcgc gcgcttccgc gctcctacct gagcgggctc
     1801 aatttcctgc tcaacgagca gcccgacaag gcgatcgacg ccttcgtcga ggcggtgcgc
     1861 atcgacccgc agaccgtcga gctgcacttc gcgctcggca gcctttttcg ccgtcgcggc
     1921 gagaccgacc gcgcgatccg catccaccag ctgctcgtcg accgcgagga catcagtgac
     1981 gagcatcgcc tgcaggcgct cggcgagctt ggccaggatt ttctcaaggc cggcctgctg
     2041 gaccgcgcgg aggctgcctt tctgcgcctg cgcggcacgc gcgccaacga tgtcgcgctg
     2101 cgctatctgc tcgagatcta ccagcaggag aaggactggg ccaaggcgat cgaggtcgcc
     2161 gaggcgctgc ctggccatga aggcgtgatg tggcacaccg aggtcgccaa cttccattgc
     2221 gagctcgctg ccacggcact ggcgaactcg cgccacgacg aggcgcgggg ccatctcgat
     2281 cgtgccttcg aggtcaatcg ccgctgcgtg cgcgcgagcc tgctgctcgg cgacctgcat
     2341 gccgcccagg gccgtgacga ggaagcgctg gaagcctggc agcgcatcga aaaccaggac
     2401 cccaattacc tcgccctcgt cgccgagcgc gtcatggacg cctgcgggcg cctcggtcgc
     2461 gtggctcagg ggcaccagct gttgcgcgcc tggctcgccg ggcacgcctc gctcgacctg
     2521 ctcgacgagc tcttccactg ggagctcgag cgcgaggggc ccaaggcggc ctacgagatg
     2581 gtgcgcgagg agctgcgccg caaccccacg ctgctcgggc tcgacaagct gctcgaggcg
     2641 gcggcgctca acgcgccggc cgagcagcgc gccgacatcg acctcatcaa gcagctcatc
     2701 cacggtcaca cccgacgcgt cgcacgctat cgctgcaaca cctgcggctt caaggcgcgc
     2761 cagttccact ggcgctgccc ggcctgcggc ggctgggaga cctatccacc gcggcgcacc
     2821 gaagagttcg acctgacgcc ctgaagcgcg gcgaacccgg cgggccggtc gggagtccac
     2881 ctcccgacga accctcccga agaaccccac tccacagggt agaaatggaa ttcaggacgc
     2941 tggaagacta cgtcggccgc acgccgctgg tgcgcctcaa gcgcatcgac gccggccgca
     3001 acaacgtgat cctcgccaag ctcgagggca acaacccggc cggctcggtc aaggatcgcc
     3061 cggcgctgtc gatggtgatg cgcgccgagg cgcgcggcca gatcaagccg ggcgacaccc
     3121 tgatcgaggc caccagcggc aacaccggga tcgcgctcgc gatggccgcg gcgatccgcg
     3181 gctaccgcat gatcctggtg atgccggaga accagagcgt cgagcgccgt cagaccatgc
     3241 gcgcgttcgg cgccgagctc gtgttggtgc cgagttcggg cggcatggag atggcgcgcg
     3301 acatcgccga gaagatgcgc gacgagggcc gcggcatcat cctcgaccag ttcgccaacc
     3361 cggacaaccc ggtggcgcac tacgagggta ccggccccga gctgtgggag cagactggcg
     3421 gccgcatcac ccactttgtc agctcgatgg gcaccaccgg caccatcatg ggcacctcgc
     3481 gcttcctcaa ggagcgcaat cccgacatct gcatcgtcgg ctgcgagccg gaggagggct
     3541 cgtccatccc cggcatccgc aagtggcccg aagcgtactt gccgaagatc ttcgagcgcc
     3601 cgcgggtcga tcgcttcgag cgcgtcagcc aggctgacgc cgaggagatg acccgccggc
     3661 tcgcgcgcga ggagggcatc ttcgcgggca tctcctcggg tggcgcgatg catgtcgcgc
     3721 tgcgcatcgc cgcgcaggtc gagaacgcgg tgatcgtctc catcgtctgc gaccgcggcg
     3781 accgttacct gtcgacgggt gtgttcccgg cctgagcgcc ttcggctggc cgggccgcgg
     3841 gtgacgcggc gatcgcgtcg cgtggaggct gcaggtgccg aacgggagtc gtcccgccgc
     3901 ggtcgcggct tgcgcgtggc gtcctgcgcc ctcttgctgc tgatgctggc gaccgccgtt
     3961 ccgcttgcgg gcgcaacggt gtttcgggtg ctcgccctcc agatcgccga gatcacccgt
     4021 cccggccttg ctgcggagga gctgagcgtg cgcttcgacg cgcatgcgca aagcggggag
     4081 atcgcggtcg gccgcctgcg ggtcggtgcg cgcgagtggc gcgcgctctc gctgcgttgc
     4141 ggtcgcctgc atctcgacga cggcgtgctc gggtgcagcg cggcacggtt ggagctgcgc
     4201 gggcggagat tgcccttcga ggccgacatc gaggcgacgc tcggccccgg gccggcgcgg
     4261 atcgtgctgc gcctggccga aggcggccgc atcgaggctg ccattcaagc ggatggcagc
     4321 ttgcgcgccc gtctgcaccg gatccgccct gcggcgaccg cggccctgct ggagccctgg
     4381 ctcgccgagc tcgccgcgcg cctgcgcgag ctcgaggccg tcggcgtgct cgatgccgag
     4441 ctcgattacc ggcccgcggg tgtgggcgac gcatccgcaa ccctgcgcgg ccggatcgcg
     4501 ggcggcggct tcggaagtgg cgacggtctg cgtgcagccg agggcgtgga ggccggtttc
     4561 acgctcgatg cacgtgggac gggcgcggcg tggtcctggg cggcgcagct cgactgggac
     4621 gcgggcgcgg cctatgtgca tccgctgctc ttcgaggccg gcccgcggct gcgcgcgcac
     4681 gggcgcttcg acggccgccg gatcgtgctc gagcgcggcg agctggcgct cgatggggtt
     4741 gcagccgcct ccgcgcgcgg cgtgttcgac accttcgccg gcacgctgga caccctcgcg
     4801 gtcgagctga ccgatgtcga tctcgtccgc atcggcccgc gctggctggc gccgctgctg
     4861 gcgccggcga gtgcgcagcg gttgcgcctc gaggggcggg cggacctgcg tgccgaattg
     4921 cgccgggatc ggctggagac ggtcgacgtg gaactgcatg gggcgggctt cggtctcgcg
     4981 tcgccgcagg gcgagggaat cgccgcaggc ggcgtagcgg aggaggcgcg cgccgaggtg
     5041 ctcgccttcg gccccgtgga tgggcgcgtg cgctggtcct cgcgcggccg gggcgaggcc
     5101 gccctgcagg ttggcggagg gcgctgggag aagctcgcgc tcggggcctt cgcgctcgac
     5161 gcggtgctcg ggcccgccgg gctgcgcctg ccgggcgcgc gcatcccctt gctcgacggt
     5221 gcgctggtgc tccgggatct cgccctcggc tggtccgtcg caggctggga ggggagcgct
     5281 ggcgcggtcc tcgagccggt gtcgatgccc ttgctcaccg aggcgctcgg tctgccgcgc
     5341 atggcggggg tgatgtcggc cgcgctgccg ggcttgcgcc tgcgtccggg cgagctcgtg
     5401 ctcgacggca cgctcgcggt gtccgtcttt ggcgggtggg tgcaggcgag cggactgcgc
     5461 gtgcacgaac ccttcggcgt cgcttcgcac ctgacgggcg agatcgaggc gcgccacatc
     5521 gatctcgccc agcttaccga ggccttcagc ttcggcagca tcaccggtca tatcgacgcc
     5581 gatgtgcgcg gcctggagct gtcgagctgg cggcccacgg ccttcgacgc ccgcatcgcc
     5641 agcatcgccg gcgacgagcg ccgccgcatc agccagcgcg cggtgcagaa cctcggcgcg
     5701 ctcggcggcg ggggcgcgct cgcggcggtg cagcgcagcg tgctgcgcct gttcgagact
     5761 ttcggctatc gcgagctcgg cctgcgctgc cggcttgcca agggcgtatg cgagatggac
     5821 gggctggatg cggcgccgga cggcgccgcg cgcgccgatg gcggcttcac catcgtgcgc
     5881 ggcggaggcg tcccggcact cgacgtcatc ggctacaatc ggcgcgtcga ttggaacgaa
     5941 ctggtggccc gcctgcagcg cgtggtggcc gagggcgtct ctcccacgat cgattgaatc
     6001 tgcatatccc gcgaggacgg catgaaccct gttgcgcgct ggctgttcgc ggctgcgctg
     6061 gttgcgagca ccgcggcctg cgtcacgatc aacatctatt tcccggccgc ggcggccgag
     6121 aaggccgccg accggatcat cgacgaggtc tggcagctgc gcgagggtgc gggcagcgcg
     6181 cctgtcacgc cgccggccgc gggagacgcg aaatgaaatc cgcttcgatg ccgtccggct
     6241 cctgggcggg ccgcctggcg gccctgttcg tcggcctcgc gctcgccttc ccggtgcatg
     6301 cgcagggcaa cctcgagatc gacaccccgg cgatctccgc gctgcgcaag acgatgcagc
     6361 agcgccacgc gcagctcgcg ccgctgtatg cgtcgggtgc ggtgggcatc gccgcagacg
     6421 gtaccgtgag cctgcgcgac gccgcggcgg tgccgctcgc gcagcgtgcc caggccaacg
     6481 ccgcggttgc cgccgaaaat gccgaccgtg ccgcgctcta ccgcgagatc gcgcgcgcca
     6541 acggtcaccc cgagtgggag gccgaggtgc gccgcacctt cgcgcagcgt tggctcgagc
     6601 gcgcgcagcc gggctggtgg gtgcagcagg gcgctgcgtg gtcgaggaaa tgatgccggt
     6661 cctcgtcttc gacatcgaaa ccattcccga cgtcgccggc atccgcgcga tcgagaacct
     6721 gcccgcgggc ctgtccgact acgaggtcgc ggagtgggcg ttccagcagc gccgcgccag
     6781 ccacggcaac gacttcctgc cgcaccatct gcagcgcgtg gtggcgatct cctgcgtgct
     6841 gcgcgacgcg cagcatttcc gggtgttctc gctctccgag cccgagagcg gcgagggcga
     6901 gatcatccag cgcttcttcg acggcatcga gcgctactcg ccgcagatcg tgtcgtggaa
     6961 cggcggcggc ttcgacctgc cggtgctgca ctaccgcggc atgctgcacg gcgttgcggc
     7021 accgcgctac tgggaccagg gcgagggcga ctaccacgac tcgcgcgact tcaagtggaa
     7081 caactacatc agccgctacc atagtcgtca tctggatctg atggacttgc tggcgctcta
     7141 ccagccgcgc gcgagcgcgc cgctcgacga cctcgccaag ctcatgggct atcccggcaa
     7201 gctcggcatg gacggttcgg cggtctggca ggcctggcag gagggccgga tcgccgagat
     7261 ccgcgactac tgcgagaccg acgtcgtcaa cacctacctc gtctacctgc gcttcgagcg
     7321 catgcgcggg catctcgacg ccgccgcgtg ggcggcggag gtggagacgg tgcgcgaggc
     7381 gctcggccgc atcgacgcac cgcactggaa gcagttcctc gccgcctggc cgcaaggctg
     7441 agcggcgcgg cgccggctcc atcccccatc ctcttacgga gagcatcatg ggcatcatcg
     7501 gaaccatcct catcggcctg atcgtcggct tcgtcgcgcg cctgctgcac cccggcaagg
     7561 acggcctcgg gatcatcatg acctcgctgc tcggcatcgc cggctcgctg gtcgccacct
     7621 acggcggcca ggcgctcggc atctatcacg ccggcgaggg cgcgggtttc ctcggtgcgg
     7681 tcatcggcgc ggtggtgatc cttttcgtgg tgacccgcct caagaaatga tcccgttccg
     7741 cgacactgca aaaaaacttt gacaagttct tcgcagtgtc tataatgcgg gacttcagca
     7801 ggcgcgtagc tcagttggtt agagcaccac cttgacatgg tgggggtcgt tggttcgagt
     7861 ccaatcgcgc
//
LOCUS       DAOQBS010000136         7706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_9111_length_7706_cov_17.006143, whole genome shotgun sequence.
ACCESSION   DAOQBS010000136 DAOQBS010000000
VERSION     DAOQBS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9111_length_7706_cov_17.006143"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKN25_12865"
     CDS             <1..224
                     /locus_tag="PKN25_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HNV91837.1"
                     /translation="ERFGLWISFYPFSQDEYLDIVAHWLRSFGASDDEISAARQEALQ
                     WALMRGSRSGRVAWQFARDHAGRLQDDAR"
     gene            221..1171
                     /locus_tag="PKN25_12870"
     CDS             221..1171
                     /locus_tag="PKN25_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HNV91838.1"
                     /translation="MTRKRVEVAAGVLLREDGCYLLGQRAPDAVYAGYWEFPGGKVEP
                     GESPAQALVRELDEELGIRVTRLRPWLCREHLYEHAHVRLHFHEVAAWEGELADRVHS
                     ALAWVRPEGPAREPMLPANGPILKALRLPRTMGITQAAEIGVEVQLDALERALAGGLR
                     LVQVREATLPDAAREAFARAALARVRAHGGLLVVNEDEVLARRIGADGVHLPARRLMA
                     VQARPDFPWVGASCHDRAELERAAALELDYALLGPVQPTLTHPGQAGMGWEGFASTAA
                     GLPLPVFALGGLAADDMERARDAGAHGIAAIRAIWRPVRD"
     gene            complement(1221..1442)
                     /locus_tag="PKN25_12875"
     CDS             complement(1221..1442)
                     /locus_tag="PKN25_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNV91839.1"
                     /translation="MDTRARTVRCPTCGKDVEWKPESRWRPFCSARCKQIDLGEWASE
                     GYRVPSSPPDAPLDEIDYQPPGAGEMPQR"
     gene            complement(1445..2200)
                     /gene="zapD"
                     /locus_tag="PKN25_12880"
     CDS             complement(1445..2200)
                     /gene="zapD"
                     /locus_tag="PKN25_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HNV91840.1"
                     /translation="MISYEYPLNERIRTLLRLEDLLLKIAHFTAGTAPQDHHVALLTI
                     FETLEVASRADLKVDLVQELERQRQILMSFRNNPEISEEALAGALYEIEQASTGLLAM
                     AGKIGQYLRENEWLMSIRSRAAIPGGVCQFDLPSYHYWLNRDPAVRRGDLGTWIQPIA
                     PIRDGIEIVLRLLRASGHPEPLHARAGAFQLTMAGRTAQMLRVRLAQSETVVPEISAN
                     KYALNVRFMLPETVARPRVAERDVAFELTFCSL"
     gene            complement(2286..2900)
                     /gene="coaE"
                     /locus_tag="PKN25_12885"
     CDS             complement(2286..2900)
                     /gene="coaE"
                     /locus_tag="PKN25_12885"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326473.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNV91841.1"
                     /translation="MSAKRPYIVGLTGGIGSGKSAATDRFAALGASVVDTDRIAHELT
                     APGGGAIEAIHVAFGDAMIAPDGRLDRAAMRALAFREPDARRRLEAILHPLIRAESAR
                     QCLAASGPYVILAVPLLVESGGYRERCDRICVVDCPEALQVARVRARSGLVDEQIRAI
                     MAAQASRAQRLAAADDVLDNAGTLASLHAQVDRLHAAYLAAATH"
     gene            complement(2914..3786)
                     /locus_tag="PKN25_12890"
     CDS             complement(2914..3786)
                     /locus_tag="PKN25_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNV91842.1"
                     /translation="MTTLRFLLILLLILNALAFAAIGGWLGGEPPAGEAGRIAAQLHP
                     ERIRLANETGATGLAPVAAEPPPPAAVVARAPSAPEATAAAPPDTTTGNAPARAATDD
                     ATLPAAQVAPEPAHPPAAEIAPAAEPPPAEPALVPAPSPAPPALCHVWADLGASEADR
                     LAQRLRRIGITAARSRSETPEAWWVRIPPQRNRSEAERRVVELNLLGVSDTFIVQESG
                     PNQNAVSLGLFKTENRARILLGQLRAKGVENAGIEPRMGTSYRIQANVPANELRIVES
                     AAPGLRARRQACTR"
     gene            complement(3864..5228)
                     /locus_tag="PKN25_12895"
     CDS             complement(3864..5228)
                     /locus_tag="PKN25_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNV91843.1"
                     /translation="MEATRILLDAADIPTHWYNVAADLPTPLAPPLGPDGHPATPEQM
                     GVIFPPAILEQEMSTERWIAIPQEVREIYRLWRPSPLCRAVRLEQALGTPAKIFFKYE
                     GVSPAGSHKPNSAVPQAFYNKQAGITRLTTETGAGQWGSSIAFAGQMFGLEVRIYMVK
                     VSYDQKPYRRLMMQTWGGEVFASPSPHTETGRRLLAENPDNPGSLGIAISEAVEEAAG
                     RADTNYTLGSVLNHVVLHQSIIGLEAKKQLDKVGLYPDVVIGPCGGGSSFAGIAFPFL
                     ADKAAGDKRAATLRCVAVEPTSCPTLTKGQYAYDFGDASGFTPLMKMYTLGHDFMPPG
                     IHAGGLRYHGDSPLVSNLLHAGLIEAAAVPQLATFEAGVQFARAEGIIPAPESCHAIR
                     QAIDEALACKATGEAKTILFNLTGHGHFDMSSYERYFSGKLEDFDYPAEAVATSLAHL
                     PKVG"
     gene            complement(5255..5992)
                     /locus_tag="PKN25_12900"
     CDS             complement(5255..5992)
                     /locus_tag="PKN25_12900"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNV91844.1"
                     /translation="MILLVDAGNTRVKWRVIAADAPARALAEGAVGHAEIDMLAAACR
                     AWPGLARVFGCNVAGTSVAARIATACAPLRVDWLLPTAARAGVRNLYENPERLGADRW
                     AALIGARHSHAGPCLVVTAGTATTVDLLSAAGDFLGGLILPGVELMQQALARGTAQLP
                     LAEGHFAWQPRRTVDAIRSGCLQAQTGAVERMFRQIAEHPGALCLLGGGAADSFADLL
                     EIPLRRTDNLVLTGLGTVATLPAPPTH"
     gene            complement(5989..6771)
                     /locus_tag="PKN25_12905"
     CDS             complement(5989..6771)
                     /locus_tag="PKN25_12905"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNV91845.1"
                     /translation="MASLLGARARDFALEWVECCDSTNARLVDAPPADDGRVHVLVAD
                     RQTAGRGRRGRQWLSWEGASLTFSLLWRFAPGTPAPAGLSLAAGLALARALEQLGVEG
                     VQLKWPNDVLVHGEKLAGILVELLPVRGRPPAAVIGIGLNLRLPADAAIPDQAGVTDL
                     AAALGGEPPSRPVLLAAILGEFHRLFDTYAAAGFPALRGAWEQRNAFAELPVAVRGES
                     ATLQGTCAGVDDDGALLLRTGDGVQRVLAGDVSLRPLLDGSR"
     gene            complement(6955..>7706)
                     /locus_tag="PKN25_12910"
     CDS             complement(6955..>7706)
                     /locus_tag="PKN25_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV91846.1"
                     /translation="FEQADGGTLFLDEIGDMPAELQTRLLRVLSDGHFYRVGGQQPIR
                     ANVRVIAATHQDLEERVRQGLFREDLFHRLNVIRLRLPPLRERHEDIPLLVRHFLQKS
                     AQELGVERKRISEATLEYLQAQPFPGNVRQLENLCHWLTVMAPAQVVEVADLPPEMRE
                     QPGRESPSNWMEGLGSEADRLIASRPGEVFDRLTRDFERTLIRRALAATGGRRIEAAQ
                     LLGIGRNTISRKIQELGMDEERAPETEESGR"
BASE COUNT         1009 a         2564 c         3020 g         1113 t
ORIGIN      
        1 ccgagcgctt cggcctgtgg atctccttct accccttcag ccaggacgag tacctcgaca
       61 tcgtcgcgca ctggctgcgc agcttcgggg cgagcgatga cgaaatctcc gccgcccgcc
      121 aggaggcgct gcagtgggcg ctgatgcgcg gctcgcgttc gggccgcgtc gcctggcagt
      181 tcgcccgcga ccatgccggt cgcctgcagg acgacgcgcg atgacgcgca agcgggtgga
      241 ggtcgccgcc ggcgtgctgc tgcgcgagga cggttgttac ctgctcggcc agcgcgcgcc
      301 ggatgcggtg tatgcgggct actgggagtt tcccggcggc aaggtcgagc ctggcgagag
      361 cccggcgcag gctctggtcc gcgagctcga cgaggaactc ggcatccgcg tcacgcggct
      421 gcggccctgg ctgtgccgcg agcacctcta cgagcatgcc cacgtccgcc tgcacttcca
      481 cgaggttgcg gcctgggagg gcgagctcgc cgaccgggtg cacagcgccc tcgcctgggt
      541 gcgtcccgag ggccccgcgc gcgagccgat gctgcccgcc aacggaccga tcctgaaggc
      601 tctgcgcctg ccgcgcacga tgggcatcac ccaggcggcg gagatcggcg tagaggtcca
      661 gctcgacgcg ctcgagcgtg cgctcgccgg cggtctgcgc ctggtgcagg tgcgcgaggc
      721 gacgctgccc gatgctgcac gcgaggcctt cgcgcgcgcg gcgctggcgc gcgtgcgggc
      781 gcatggcggg ctgctggtgg tgaatgagga cgaggtgctc gcgcgccgca tcggcgccga
      841 cggcgtgcat ctgccggcgc gccggctgat ggcggtgcag gcgcgaccgg atttcccctg
      901 ggtgggggcg tcctgccacg accgcgcgga gctcgagcgc gccgcggcgc tggagctcga
      961 ttacgccttg ctcggaccgg tgcagccgac cctgacccat cccggccagg cggggatggg
     1021 ctgggaaggg ttcgcgagca cggcggcggg cctgccgctg ccggtgttcg ccctcggcgg
     1081 gctggcagcg gatgacatgg agcgtgcgcg cgatgccggc gcgcacggca tcgccgcgat
     1141 ccgcgcgatc tggaggcccg tccgggattg aaggcgcacg cgccgcgagg cggtggcgaa
     1201 tccgcccgcg ccggcggcgt tcagcgctgc ggcatctcgc ccgcgccggg cggctggtag
     1261 tcgatctcgt cgagcggcgc atcgggcggc gaactcggca cacggtagcc ttccgaggcc
     1321 cactcgccga ggtcgatctg cttgcagcgc gccgagcaga acgggcgcca gcggctctcc
     1381 ggcttccatt cgacgtcctt gccgcaggtg gggcagcgca cggtgcgggc ccgggtgtcc
     1441 atgttcacag gctgcagaag gtcagttcga aggcgacgtc gcgctcggcc acgcgtgggc
     1501 gtgcgacggt ctcgggcagc atgaagcgca cgttgagtgc gtacttgttg gcgctgattt
     1561 cgggcaccac ggtctcggac tgcgccagac gcacgcgcag catctgtgcg gtgcgccccg
     1621 ccatggtgag ctggaaggcg ccggcacgtg cgtgcagcgg ctcggggtgg ccgctggcgc
     1681 gcagcaggcg cagcacgatc tcgatgccat cgcggatcgg cgctatcggc tggatccagg
     1741 tgccgaggtc gccgcggcgc acggccgggt cgcgattgag ccagtagtgg taggacggca
     1801 ggtcgaactg gcagactccg cccgggatcg ccgcgcggct gcggatgctc atcagccact
     1861 cgttctcgcg caggtactgg ccgatcttgc ctgccatcgc gagcagcccg gtggaggcct
     1921 gctcgatctc gtagagcgcg ccggcaagcg cttcctcgga gatctcgggg ttgttgcgga
     1981 aggacatcag gatctggcgc tggcgctcga gttcctgcac caggtcgacc ttcaggtctg
     2041 cgcggctcgc cacttcgagc gtctcgaaga tggtcagcag cgcgacatgg tggtcttgcg
     2101 gcgccgtgcc cgcggtgaaa tgggcaatct tgaggagcag gtcctccagg cgcagcaagg
     2161 tgcggatgcg ctcgttgaga ggatattcgt agctaatcac cgcagcgtgt ccgcctgtat
     2221 cgcacctgtt cagagtcgcg ccgatgatag cacaggcccc cttcccacca tggcgaggca
     2281 cggtttcagt gcgtcgccgc ggcgaggtag gcggcgtgca ggcgatcgac ctgtgcgtgc
     2341 agggatgcga gcgtgccggc gttgtcgagc acgtcgtcgg cggcggccag acgttgcgcc
     2401 cgactggcct gcgcggccat gatggcgcgg atctgttcat cgacgagccc gctgcgcgca
     2461 cgcacgcgcg cgacctgcag cgcctcggga cagtcgacca cgcagatgcg gtcgcagcgc
     2521 tcgcggtagc cgccagactc gaccagcagc ggcactgcga ggatcacgta ggggccgctg
     2581 gccgccaggc actggcgggc gctctcggcg cggatcagcg ggtgcaggat ggcctcgagg
     2641 cggcggcggg catcgggctc gcggaaggcg agcgcgcgca tggcggcgcg gtcgaggcgg
     2701 ccatcgggcg cgatcatggc gtcgccgaag gcgacgtgga tggcctcgat cgcgccgccg
     2761 ccgggggcgg tgagctcgtg ggcgatacgg tcggtgtcga ccacgctcgc gccgagggcg
     2821 gcgaagcggt cggtggcggc gctcttgccg ctgccgatgc cgccggtgag gccgacgatg
     2881 taggggcgct tcgcgctcat ctccttctcc gaatcagcgc gtgcaggcct ggcgccgggc
     2941 gcgcaggccg ggcgcggccg attcgacgat gcgcagctcg ttggccggga cgttggcctg
     3001 gatgcggtag ctggtgccca tgcgcggctc gatgccggcg ttctcgacgc ccttggcacg
     3061 cagttggccg agcaggatgc gggcgcggtt ctcggtcttg aagaggccga gcgagaccgc
     3121 gttctggttg gggccggact cctgcacgat gaaggtgtcg ctgacgccga gcaggttgag
     3181 ctcgacgacg cggcgctcgg cctcgctgcg gttgcgctgc ggcgggatgc gcacccacca
     3241 ggcctccggc gtctcgctgc ggctgcgcgc cgcggtgatg ccgatgcggc gcaggcgctg
     3301 ggcgaggcgg tcggcctcgc tcgcgccgag gtcggcccag acgtggcaga gggcgggcgg
     3361 cgccggcgag ggggcgggga ccagggcagg ctcggcgggc gggggctctg ccgcgggggc
     3421 gatctcggct gccgggggat gggccggctc gggtgcgacc tgcgctgcgg ggagcgtggc
     3481 gtcgtccgtg gcggctcgcg ccggggcgtt gccggtcgtg gtgtcgggtg gcgcggctgc
     3541 ggtggcctcc ggcgcggaag gcgcgcgggc gacgaccgcg gcgggcgggg gcggttcggc
     3601 ggcgacgggc gcgaggccgg tggcgccggt ctcgtttgcc agccggatac gctcgggatg
     3661 caactgcgcg gcgatgcgcc ccgcctcgcc cgccggcggc tcgccaccca gccagccgcc
     3721 gatcgccgcg aaggcgaggg cgttgaggat gagcagcagg atgagcagga agcgcagggt
     3781 cgtcatgcct tggtgtcgca gggggggcgg ggaaggcggg gcgctccgcc ggccttgccg
     3841 gccgagcgcg accgccagct cgttcagccg accttgggca ggtgggcgag cgaagttgcc
     3901 accgcctcgg ccgggtagtc gaagtcctcg agcttgcccg agaagtagcg ctcgtacgag
     3961 ctcatgtcga agtggccgtg gccggtcagg ttgaacagaa tggtcttcgc ctcgccggtc
     4021 gccttgcagg cgagcgcctc gtcgatggcc tgacggatgg cgtggcagga ctcgggcgcc
     4081 gggatgatgc cctcggcgcg tgcgaactgc acccccgcct cgaaggtcgc cagctgcggc
     4141 accgcggcgg cctcgatgag gccggcgtgc agcaggttcg acaccagcgg cgagtcgcca
     4201 tggtagcgca ggccgccggc atggattccg ggcggcatga aatcgtggcc cagcgtatac
     4261 atcttcatca gcggggtgaa gccggaggcg tcgccgaagt cgtaggcgta ctggcccttg
     4321 gtcagggtcg ggcaggaggt cggctccacc gccacgcagc gcagcgtggc ggcgcgcttg
     4381 tcgcccgcgg ccttgtcggc gaggaagggg aaggcgatgc cggcgaagct cgagccgccg
     4441 ccgcaggggc cgatgacgac gtcgggatag aggccgacct tgtcgagctg cttcttcgcc
     4501 tccaggccga tgatgctctg gtgcagcacc acgtggttga gcaccgaacc cagggtgtag
     4561 ttggtgtcgg cgcggccggc ggcctcttcc accgcctcgg agatggcgat gccgagcgag
     4621 ccggggttgt ccgggttctc ggcgagcagg cggcggccgg tctcggtgtg cggcgagggg
     4681 ctggcgaaga cctcgccgcc ccaggtctgc atcatcaggc ggcggtaggg cttctggtcg
     4741 tagctgacct tgaccatgta gatgcgcacc tccagcccga acatctgccc ggcgaaggcg
     4801 atcgacgagc cccactgccc ggcgccggtc tcggtggtca ggcgggtgat gccggcctgc
     4861 ttgttgtaga aggcctgcgg caccgcggag ttgggcttgt gcgagccggc gggcgagacg
     4921 ccctcgtact tgaagaagat cttcgccggg gtgccgagcg cctgctccag gcgcaccgcg
     4981 cgacacagcg ggctcggtcg ccacaggcgg tagatctcgc gcacctcctg cgggatggcg
     5041 atccagcgct cggtgctcat ctcctgttcg aggatcgccg gcgggaagat gacccccatc
     5101 tgctccggcg tggccggatg gccgtcgggg ccgagcggcg gcgcgagcgg cgtcggcaga
     5161 tcggcggcga cgttgtacca gtgcgtcggg atgtcggcgg cgtcgagcag gatgcgggtg
     5221 gcttccatcg ttgttttctc cctttcgatt cttgtcagtg ggtgggcggg gcgggcaggg
     5281 tggcgaccgt gcccaggccg gtcagcacca gattgtcggt tcgtcgcagc ggaatctcga
     5341 gcaggtcggc gaagctgtcg gcggcgccgc cgccgagcag gcagagcgcg ccggggtgct
     5401 ccgcgatctg gcggaacatg cgctcgactg cgccggtctg ggcttgcagg cagccggagc
     5461 ggatcgcgtc taccgtgcgt cgcggttgcc acgcgaagtg accctcggcg agcggcagct
     5521 gagccgtgcc gcgggcgagc gcctgctgca tcagctcgac cccgggcagg atcaggccgc
     5581 cgaggaagtc gcccgccgcg gagagcaggt ccaccgtggt cgcggtgccg gcggtgacga
     5641 ccaggcaggg gccggcatgg ctgtggcggg caccgatcag cgccgcccag cggtcggcgc
     5701 cgagacgctc ggggttctcg tacaggttgc gcacgccggc gcgcgcggcg gtgggcagca
     5761 gccaatccac ccgcagcggt gcgcaggccg tggcgatgcg cgcggcgacg ctggtgccgg
     5821 cgacgttaca gccgaacacg cgcgcgagtc ccggccaggc gcggcaggcg gcggcgagca
     5881 tgtcgatctc ggcgtggccg actgccccct cggcgagggc tcgcgcgggg gcgtccgctg
     5941 cgatcacgcg ccatttcacc cgggtgttgc cggcatccac gagcaggatc atcgcgagcc
     6001 gtccagcagc ggacgcagcg acacgtcgcc ggcgagcacc cgctgcacgc cgtccccggt
     6061 gcgcagcagc agggcgccgt cgtcgtccac cccggcgcag gtaccctgca gcgtggcgga
     6121 ctccccgcgg accgccaccg gtaactcggc gaaggcgttg cgctgctccc aggcgccgcg
     6181 cagggcgggg aagcccgcgg ccgcgtaggt gtcgaagagg cggtggaact cgccgaggat
     6241 ggccgccagc aggaccggac gcgagggcgg ttcgccgccg agcgcagcgg cgaggtcggt
     6301 gacgccggcc tggtcgggaa tggcggcgtc ggccggcagg cgcaggttga gaccgatgcc
     6361 gatcaccgcc gcgggcgggc gaccgcgcac ggggaggagt tcgaccagga tgccggcgag
     6421 cttctctccg tgcaccagca cgtcgttggg ccatttcagc tgcaccccct cgacgccgag
     6481 ctgctcgagc gcgcgcgcga gcgccagccc ggcggcgagc gagagtccgg ccggcgccgg
     6541 ggtacccggc gcgaagcgcc acagcagcga gaaggtgagg ctggcgccct cccaggacag
     6601 ccattgccgc ccgcgccgcc cgcgcccggc ggtctggcgg tcggccacga gcacatgcac
     6661 acgaccgtcg tcggcgggcg gcgcatcgac gagacgggcg ttggtcgagt cgcagcactc
     6721 cacccattcg agggcgaagt cgcgcgcacg cgcgccgagg agggaggcca gttcggtcgc
     6781 gcaggcgcgc gccgaaggcg tggatggacg gatggaggac aagtgcggaa gcggcggatc
     6841 gaaaaccgca ttatccgcca agaccgtggg ggatgcgctt cccgcggcat ggatgcgctg
     6901 cgccccgtgc gccggccgcg ccaacgcgcc cccgcatcga ggcgaccggg gcgatcagcg
     6961 cccgctttcc tccgtctccg gcgcgcgctc ctcgtccatg cccagctcct ggatcttgcg
     7021 gctgatggtg ttgcggccga tgccgagcag ctgcgcggcc tcgatgcggc ggccgccggt
     7081 ggcggccagc gcacggcgga tcagggtgcg ctcgaaatcg cgcgtgaggc ggtcgaacac
     7141 ctctccgggg cgcgaggcga tcaggcggtc ggcctcgctg cccaggccct ccatccagtt
     7201 cgatggcgac tcgcggccgg gttgctcgcg catctcgggt ggcaggtcgg cgacctccac
     7261 cacctgcgcc ggcgccatca cggtgagcca gtggcacagg ttctcgagct ggcgcacgtt
     7321 gcccgggaaa ggctgagcct gcaggtattc gagcgtggcc tccgagatgc gcttgcgctc
     7381 gacgccgagc tcctgcgcgc tcttctgcag gaagtgccgc accagcaagg ggatgtcctc
     7441 atggcgctcg cgcagcggcg gcaggcgcag gcggatgacg tttaggcggt ggaagaggtc
     7501 ctcgcggaac aggccctggc gcacgcgctc ctccaggtcc tggtgggtgg cggcgatcac
     7561 gcgcacgttg gcgcggatcg gttgctggcc gccgacgcgg tagaagtggc cgtcggagag
     7621 cacgcgcagc agccgcgtct gcagctcggc cggcatgtcg ccgatctcgt cgaggaacag
     7681 cgtgccgccg tcggcctgct cgaagc
//
LOCUS       DAOQBS010000137         7483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_9542_length_7483_cov_13.941976, whole genome shotgun sequence.
ACCESSION   DAOQBS010000137 DAOQBS010000000
VERSION     DAOQBS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9542_length_7483_cov_13.941976"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..454)
                     /locus_tag="PKN25_12915"
     CDS             complement(47..454)
                     /locus_tag="PKN25_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HNV91847.1"
                     /translation="MTFVLELLVSLLIVAGGSFLLIGSIGMVKLPDLLTRLHAPTKAT
                     TLGVGGALAASMVFFAGLEGTLSIHEVLITAFLFMTAPLSAHFIAKAHLHEHTELRDG
                     LPPTGRPYGWSTFDAAPGPSEPDDEDERRGAHD"
     gene            complement(468..737)
                     /locus_tag="PKN25_12920"
     CDS             complement(468..737)
                     /locus_tag="PKN25_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HNV91848.1"
                     /translation="MIEYALQFGFATASLALLLNLWRLLRGPALMDRVLAVDTMVINI
                     IALIVLAGIQQRATHFFEAALLFAMFGFISTVAYCRFILRGDVIE"
     gene            complement(734..1261)
                     /locus_tag="PKN25_12925"
     CDS             complement(734..1261)
                     /locus_tag="PKN25_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNV91849.1"
                     /translation="MGAKHDKLDQRSFTERWLPHPLMSAVLVLLWMLLLNSFSFGGLV
                     VGVVLAVVITRVTSNFWPERPPVKSWTKAFAYLGVVAWDVVVANLQVTRLILFRPVGS
                     LATRWVVVPLELRSPEAITVLAGTITMTPGTVSCDLSADGRSLLVHCLDAPDAEEAVR
                     QMKERYEDRLQEIFP"
     gene            complement(1261..2784)
                     /locus_tag="PKN25_12930"
     CDS             complement(1261..2784)
                     /locus_tag="PKN25_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HNV91850.1"
                     /translation="MNHLLILPILLPAVVGALLVLAARHDGVLARTFAVASTVLLLAI
                     AVALLVLASDGQVRSYALGAWPAPFGIVLALDRLAALMLVLNAVLGLGVLLYAIGGWD
                     QRGKHFHPLFQFELMGINGAFLTGDFFNLFVFFEILLIASYGLMVHGGGGLRVKAGVQ
                     YVVTNLVGSSVFLIAVGMIYNVTGTLNMADLARIVPQVPAADQALLQTGAVLLLLVFA
                     VKAALVPLHFWLPGTYANAPGPVAALFAIMTKVGAYSILRLYVLAFGADAGAAAWLAA
                     PWLLPAGLLTLVLGMTGVLAARSLGQMASFAVIGSMGMLLATVAVFTPQATSAALYYL
                     IHSTLAAGALFLVADLVAERRGAQRDRFAAAPRIAQGGLVGGLFFVAAIAMTGMPPLS
                     GFIGKLLVLDGVRASAHAAWFWALILTTSLIAIVGFARAGSFVFWKAHALPAAPASAY
                     RPDGALSFVAVGGLLACLVLLTVFAGPVHAWLELVAAQLYAPAGYIDAVLGTAQGVR"
     gene            complement(2781..3197)
                     /locus_tag="PKN25_12935"
     CDS             complement(2781..3197)
                     /locus_tag="PKN25_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HNV91851.1"
                     /translation="MEFLVASAIGVMTAAGIYMVLRARTFPVVIGLSLLTYATNLFLF
                     ATGRLAIGQPPVISAAATGYIDPLPQALVLTAIVISFGMTAVVVVMALRAYLETGNDH
                     VDLPGDASPEAAEDDWKLRARKPAPATARSGRGERA"
     gene            complement(3197..6058)
                     /locus_tag="PKN25_12940"
     CDS             complement(3197..6058)
                     /locus_tag="PKN25_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HNV91852.1"
                     /translation="MSLALIVLLPFIGALFPALMIRAGRSACATTAFVFPLLALVLLL
                     AQAPAVFRGEVLVAAWDWVPALGLQAGFFLDGLGMFFAGLILGIGLLVIVYARFYLAK
                     SDPMGVFYSYLLLFQGAMVGVVLSDNILLLLVFWELTSLSSFLLIGYWKHLPEGRQGA
                     RMALTVTGAGGLAMIAGMLILGDIAGSWNLSDILRNKEAIQASPLYVPALVLILAGCF
                     TKSAQFPFHFWLPHAMAAPTPVSAYLHSATMVKAGLFLMARMWPVLAGTTEWFYLVAT
                     TGLVTMLVAAGIALFKDDLKGLLAYSTVSHLGLVTMLLGFGTPLAAVAAVFHILNHAT
                     FKAALFMNAGIVDHEAGTRDIRRLGGLLQLMPITATMAMVAGASMAGLPPLNGFLSKE
                     MMLHEAVDTTWLGNGWIVAALATLAACFSVAYSFRYIVHVFFGARRDDDPHTPHDPPA
                     GMWLPPALLVVLVVAIGLFPDSVAGPLVAVVARAVTGGAVMDYHLALWHGFTPALGLS
                     AIAVAVGLGALVAYRRVRALWQAGPHPEAKAMFDAFIEGAAALARGITHGLHNGSLQR
                     YLAFALTAFTLAGFAAFLAAPHAAGTRALLPAHPAAIAAWVLLVGGCALTVMLFRRRV
                     LSVLVVGTVGLIVCLAFIYLSAPDLALTQISVEVATVILILLALYFLPKEGPQSSSVR
                     ADPLRHLRDGVLAIVAGIGMAGASWAMLTRDGSSLSSYYLDNSVSGGGGTNVVNVILV
                     DFRGFDTFGEITVLGIAALAIYALLDGALFGRTGRRLGAWSPDRPQSADRHPMIMVVA
                     TRVMLPLAMLVGAYIFLRGHNQPGGGFIAALVVSIALIMQYMASGFGWAAHRVKVNYH
                     AMIGLGVLVAAATGIGAMVLDQPFLTSTFGHFHLPLVGEFELASAMAFDTGVFLTVVG
                     AVMLALANLSRMGRWTSPYTINTGAMDVDPRAASGKEQG"
     gene            6282..7082
                     /locus_tag="PKN25_12945"
     CDS             6282..7082
                     /locus_tag="PKN25_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNV91853.1"
                     /translation="MQPKKTESAPSAEGKTSIQVIERMMKLLDVLAEHADPVQLKQLA
                     IETGLHPSTAHRILGAMTHSGFVERSEAGAYRLGIRLLELGSLVKSRISLRETAMPYM
                     LKLHAATGESVNLGVRAGDEIVYVERTSSGRASVRVVHIVGARAPLHTTATGKLFLVE
                     DGLERIRDYARRTALPASTPASITALPALEKELDRVRRHGVAFDLDEVEAGVRCIAAG
                     IRDDSGELIAGLSLSTPSERFNPDWAPLVRETADEISRALGHLPPKAG"
BASE COUNT         1298 a         2498 c         2815 g          872 t
ORIGIN      
        1 ggggctgtgg gagcgacgcg agtcgcgatc gggcgactgg cggggctcag tcgtgggcgc
       61 cgcggcgctc gtcttcgtcg tcgggctcgc tggggccggg ggcggcgtcg aaggtgctcc
      121 agccgtaggg gcggccggtg ggcgggaggc cgtcgcgcag ttcggtgtgc tcgtgcaggt
      181 gggccttggc gatgaagtgg gcgctgagcg gtgcggtcat gaacaggaag gcggtgatca
      241 gcacctcgtg gatggacagc gtgccctcca gcccggcgaa gaacaccatg gacgcggcga
      301 gcgcgccgcc cacgccgagc gtggtcgcct tggtgggcgc gtgcaggcgg gtgagcaggt
      361 cgggcagctt gaccatgccg atggagccga tgagcaggaa gctgccgccg gcgacgatca
      421 gcagcgagac gaggagttcg agcacgaagg tcatgagggg tctccgctca ctcgatgacg
      481 tcgccgcgca ggatgaagcg gcagtaggcg acggtggaga tgaagccgaa catcgcgaac
      541 agcagcgcgg cctcgaagaa gtgggtggcg cgctgctgga tgccggcgag cacgatcagc
      601 gcgatgatgt tgatgaccat ggtgtcgacc gcgagcacgc ggtccatgag cgcggggccg
      661 cgcagcagcc gccacaggtt gagcagcagc gcgagcgagg cggtggcgaa gccgaactgc
      721 agggcgtatt cgatcacggg aagatctcct ggaggcggtc ctcgtagcgt tccttcatct
      781 ggcgcacggc ctcctcggcg tcgggcgcgt cgaggcagtg cacgagcagg ctgcggccgt
      841 cggcggagag gtcgcaggac accgttccgg gcgtcatcgt gatggtgccg gcgagcacgg
      901 tgatggcctc gggcgagcgc agctcgagcg gcaccaccac ccagcgcgtg gccaggctgc
      961 ccaccggccg gaacaggatc aggcgggtga cctgcaggtt ggcgaccacc acgtcccagg
     1021 ccaccacgcc gaggtaggcg aaggccttgg tccaggactt gaccggcggg cgctcgggcc
     1081 agaagttgct cgtcacccgc gtgatcacca ccgcgagcac cacgcccacc accagcccgc
     1141 cgaagctgaa actgttgagc agcagcatcc acagcagcac cagcaccgcg ctcatgagcg
     1201 gatgcggcag ccagcgttcg gtgaagctgc gctggtcgag cttgtcgtgc ttcgcgccca
     1261 tcagcgcacc ccctgtgcgg tgccgagcac cgcgtcgatg tagccggccg gggcgtagag
     1321 ctgcgccgcg acgagctcca gccaggcgtg caccgggccg gcgaacacgg tgagcagcac
     1381 caggcaggcg agcagcccgc ccacggcgac gaaggacagc gcgccgtccg ggcgataggc
     1441 ggacgcgggc gcggcgggca gcgcgtgcgc cttccagaac acgaagctgc cggcgcgcgc
     1501 gaagccgacg atggcgatca gcgaggtcgt caggatcagc gcccagaacc aggccgcgtg
     1561 cgccgaggcg cgcacgccgt cgagcaccag cagcttgccg atgaagccgg acagcggcgg
     1621 catgccggtc atggcgatgg cggcgacgaa gaacaggccg ccgaccagcc cgccctgggc
     1681 gatgcgcggc gccgcggcga agcggtcgcg ctgcgcgccg cggcgctcgg cgacgaggtc
     1741 ggcgacgagg aagagcgcgc cggcggcgag cgtggagtgg atgaggtagt acagcgcggc
     1801 gctggtcgcc tgcggggtga acaccgccac ggtggcgagc agcatgccca tcgagccgat
     1861 caccgcgaag ctggccatct ggccgaggct gcgcgcggcg agcacgccgg tcatgcccag
     1921 caccagggtg agcagcccgg ccggcagcag ccagggcgcg gcgagccagg ccgcggcccc
     1981 ggcgtcggcg ccgaaggcga gcacgtacag gcgcaggatg gaataggcgc ccaccttggt
     2041 catgatcgcg aacagcgccg ccaccgggcc gggcgcgttg gcgtaggtgc cgggcagcca
     2101 gaagtgcagc ggcacgagcg cggccttgac cgcgaacacc agcagcagca gcaccgcgcc
     2161 ggtctgcagc agcgcctggt cggccgccgg cacctgcggg acgatgcgcg cgaggtcggc
     2221 catgttgagg gtgccggtga cgttgtagat catgccgacc gcgatcagga acaccgagga
     2281 gccgaccagg ttggtgacca cgtactgcac gcccgccttc acccgcagcc cgccgccgcc
     2341 gtgcaccatg aggccgtagg aggcgatcag caggatctcg aagaacacga agaggttgaa
     2401 gaagtcgccg gtgaggaagg cgccgttgat gcccatcagc tcgaactgga agagcgggtg
     2461 gaagtgcttg ccgcgctggt cccagccgcc gatcgcgtac agcagcacgc cgaggccgag
     2521 gacggcattg agcaccagca tgagcgccgc caggcggtcg agcgcgagca cgatgccgaa
     2581 gggcgcgggc caggcgccga gcgcgtagct gcgcacctgg ccgtcgctgg cgagcaccag
     2641 cagcgcgacg gcgatggcga gcagcagcac ggtggaggcc accgcgaagg tgcgcgcgag
     2701 cacgccgtcg tggcgcgcgg ccagcaccag cagcgcgccg accacggccg gcagcaggat
     2761 ggggaggatc agcaggtggt tcatgcgcgc tccccgcgac cgctgcgcgc cgtcgccggc
     2821 gccggcttgc gggcgcgcag cttccagtcg tcctcggcgg cctcgggcga ggcgtcgccg
     2881 ggcaggtcga cgtggtcgtt gccggtctcg aggtaggcgc gcagcgccat caccaccacc
     2941 accgcggtca tgccgaagga gatcacgatc gcggtgagca cgagcgcctg cggcagcggg
     3001 tcgatgtagc ccgtggcggc ggcggagatc accggcggct ggccgatcgc caggcggccg
     3061 gtggcgaaca ggaacaggtt ggtggcgtag gtgagcagcg acaggccgat caccaccggg
     3121 aaggtgcgcg cgcgcagcac catgtagatg ccggccgcgg tcatcacgcc gatggcgctg
     3181 gcgacgagga attccatcag ccctgctcct tgcccgaggc ggcgcgcgga tccacgtcca
     3241 tcgcgccggt gttgatggtg taggggctgg tccagcgccc catgcgcgag aggttggcga
     3301 gcgccagcat gaccgcgccg accacggtga ggaagacccc ggtgtcgaag gccatcgcgc
     3361 tggcgagctc gaactcgccc accagcggca ggtggaagtg gccgaaggtg gaggtgagga
     3421 agggctggtc gagcaccatc gcgccgatgc cggtggcggc ggccaccagc acgccgaggc
     3481 cgatcatggc gtggtagttg accttgaccc gatgcgccgc ccagccgaag ccgctcgcca
     3541 tgtactgcat gatcagcgcg atcgacacca cgagcgcggc gatgaagccg ccgcccggct
     3601 ggttgtggcc gcgcaggaag atgtaggcgc cgaccagcat cgccagcggc agcatgaccc
     3661 gggtggccac caccatgatc atcgggtggc ggtcggccga ctgcggacgg tcgggggacc
     3721 acgcgcccag acgacggccg gtgcggccga agagcgcgcc gtcgagcagg gcgtagatcg
     3781 ccagcgcggc gatgccgagc acggtgatct cgccgaaggt gtcgaagccg cggaagtcga
     3841 ccaggatcac gttgaccacg ttggtgccgc cgccgcccga gaccgagttg tcgaggtagt
     3901 agctcgacag cgagctgccg tcgcgggtga gcatcgccca gctcgccccg gccatgccga
     3961 tgccggccac gatggcgagc acgccgtcgc gcaggtggcg cagcggatcg gcgcgcaccg
     4021 aggagctctg cggaccttcc ttgggcagga agtagagcgc gagcaggatc aggatcacgg
     4081 tggcgacctc gaccgagatc tgcgtcagcg cgaggtcggg cgcggacagg tagatgaagg
     4141 ccaggcacac gatcagcccc accgtgccca ccaccagcac cgacagcacg cggcggcgga
     4201 acagcatcac ggtgagcgcg cagccgccca ccagcagcac ccaggcggcg atcgcggccg
     4261 ggtgcgccgg cagcagcgcg cgcgtgcccg cggcgtgggg cgcggcgagg aaggcggcga
     4321 agccggcgag cgtgaaggcg gtgagcgcga aggcgagata gcgctgcagc gagccgttgt
     4381 gcaggccgtg ggtgatgccg cgggcgagcg cggcggcgcc ctcgatgaag gcgtcgaaca
     4441 tcgccttggc ctcggggtgc ggccccgcct gccagagcgc gcgcacgcgc cggtaggcga
     4501 cgagcgcgcc caggccgacc gcgaccgcga tcgccgacag gccgagcgcc ggggtgaagc
     4561 cgtgccagag cgcgaggtgg tagtccatca ccgccccgcc ggtgacggcg cgcgccacca
     4621 ccgccaccag cggccccgcc acgctgtcgg ggaagagccc gatcgccacc accagcacca
     4681 ccagcagcgc cggcggcagc cacatgccgg cgggcgggtc gtgcggcgtg tgcggatcgt
     4741 cgtcgcggcg cgcgccgaag aagacgtgca cgatgtagcg gaacgagtag gccaccgaga
     4801 agcacgccgc cagcgtggcc agcgcggcga cgatccagcc gttgcccagc caggtggtgt
     4861 cgaccgcctc gtgcagcatc atctccttgg agaggaagcc gttgagcggc ggcaggccgg
     4921 ccatcgaggc ccccgccacc atcgccatgg tcgcggtgat cggcatcagc tggagcaggc
     4981 cgccgaggcg gcggatgtcg cgcgtgccgg cctcgtgatc gacgatgccg gcgttcatga
     5041 acagcgcggc cttgaaggtg gcgtggttga ggatgtggaa cacggcggcc accgccgcga
     5101 gcggcgtgcc gaagccgagc agcatggtca ccaggccgag gtggctgacg gtggagtagg
     5161 ccagcaggcc cttgaggtcg tccttgaaca gcgcgatgcc ggccgccacc agcatggtca
     5221 ccaggccggt ggtggcgacg aggtagaacc actcggtcgt cccggcgagc accggccaca
     5281 tgcgcgccat caggaagagg cccgccttca ccatggtcgc cgagtgcagg taggccgaca
     5341 ccggggtggg cgccgccatc gcgtgcggca gccagaagtg gaaggggaac tgcgcgctct
     5401 tggtgaagca gccggcgagg atcagcacca gcgccgggac gtacagcggc gaggcctgga
     5461 tcgcttcctt gttgcgcagg atgtcgctga ggttccagga gccggcgatg tcgcccagga
     5521 tcagcatgcc ggcgatcatc gccagcccgc cggcgccggt gacggtgagc gccatgcgcg
     5581 caccctggcg gccttcgggc aggtgcttcc agtagccgat gagcaggaag gacgacaggc
     5641 tggtgagctc ccagaacacc agcagcagca ggatgttgtc ggacaggacg acgcccacca
     5701 tcgcgccctg gaagagcagc agatagctgt agaacacgcc catcgggtcg ctcttggcga
     5761 gatagaagcg ggcgtagacg atcaccagca ggccgatgcc caggatcagc ccggcgaaga
     5821 acatgcccag cccgtcgagg aagaagcccg cctgcaggcc gagcgcgggc acccagtccc
     5881 aggcggcgac gaggacctcg ccgcggaaca ccgccggcgc ctgcgcgagc agcagcacga
     5941 gggcgaggag cggaaagacg aaggcggtgg tcgcgcaggc gctgcggccg gcgcggatca
     6001 tcagcgcggg aaagagcgcg ccgatgaagg gcagcagcac gatcagggcg aggctcatcg
     6061 gggggctcct cgggcgtggt cgatgggaaa ggcgacgtga atcgcgggtg gcgggggcca
     6121 cccacacccc gcatgaggac gaattccggg cggagtatat cggggatgcg acccccgcca
     6181 aatcggggtt gatttcacta ggcgaaatcg gatcgcacgg gtaccattcg ccagatctcc
     6241 ttgccggccc cggcaagctt ccgatccttc cccgaacctc cgtgcagccg aagaagaccg
     6301 aatccgcccc cagtgccgaa ggcaagacct cgatccaggt catcgaacgc atgatgaagc
     6361 tgctcgacgt gctggccgaa catgccgatc cggtccagct caagcagctc gcgatcgaga
     6421 ccggtctgca tccgtcgacc gcgcaccgca tcctcggcgc gatgacgcac agcggctttg
     6481 tcgagcgctc cgaggccggc gcctaccggc tcgggatccg cctgctcgag ctcggcagcc
     6541 tggtgaagtc gcgcatctcg ctgcgcgaga ccgccatgcc ctacatgctc aagctgcacg
     6601 ccgccaccgg cgagagcgtg aacctgggcg tgcgcgccgg cgacgagatc gtgtatgtcg
     6661 agcgcacctc gagcgggcgc gcctcggtgc gcgtggtgca catcgtgggc gcgcgcgcgc
     6721 cgctgcacac caccgccacc ggcaagctct tcctggtcga ggacggcctc gagcgcatcc
     6781 gcgactacgc ccgccgcacc gccctgcccg cctccacccc ggcctcgatc accgcgctgc
     6841 cggcgctgga gaaggagctc gaccgcgtgc gccgccacgg cgtggccttc gacctcgacg
     6901 aggtcgaggc cggcgtgcgc tgcatcgccg cgggcatccg cgacgacagc ggcgagctga
     6961 tcgccggcct gtcgctatcg accccttccg aacgcttcaa ccccgactgg gccccactgg
     7021 tgcgcgagac cgccgacgag atctcccgcg ccctcggcca ccttccgcca aaggcgggat
     7081 aagcccgctt gcgggcagcc cccctcgcag cggcgaaagc cgcaaggtct gcacgcggac
     7141 accgatgccc cgaggccggg tgcgggaaga ccacggcccc cggggtggga ggtggaaaaa
     7201 ccacggcgcc cggggtggga tgtgggagcg acgcaagtcg cgatcctgcg ctgcggctgc
     7261 gacaggcgtg gtcgaggcgg aaaggctgca atcgcgactt gcgtcgctcc cacggcaaga
     7321 caccgacacc cggacgcgtt gcgcgaacgc cgacacccgg atgcgttgcg accgaacacc
     7381 gatgccccga ggccgggtgc gggaagacca cggcgcccgg ggtggggggt gggaaaacca
     7441 cgacgcccgg ggtgggatgt gggagcgacg caagtcgcga tcc
//
LOCUS       DAOQBS010000138         7249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_10034_length_7249_cov_17.123436, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000138 DAOQBS010000000
VERSION     DAOQBS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10034_length_7249_cov_17.123436"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /gene="thrH"
                     /locus_tag="PKN25_12950"
     CDS             <1..146
                     /gene="thrH"
                     /locus_tag="PKN25_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321157.1"
                     /note="catalyzes the formation of serine from
                     phosphoserine; also has phosphoserine:homoserine
                     phosphotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNV91854.1"
                     /translation="DTAMLGEAHGGILFHPPENVIREFPQYPVVRDYAGLRAEIDKVF
                     ARV"
     gene            192..1067
                     /gene="purU"
                     /locus_tag="PKN25_12955"
     CDS             192..1067
                     /gene="purU"
                     /locus_tag="PKN25_12955"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNV91855.1"
                     /translation="MHRQRFFTLSASCPDRVGIVAKVSGFIAEHKGWILETSLHAEPP
                     LEAGEVGRYFMRIEIKADSLPFHLAEFREKFRPLAEELEMDWKITDSAVKKRVVVLVS
                     KQEHCLYDLLARWQSKELDIEIPCVISNHDTFRGFVEWHGIPFHHVPVGTDNKSAAYA
                     EVRRIFEEVRGDTMVLARYMQILSPDLCAAYPGRILNIHHSFLPSFVGAKPYHQAYAK
                     GVKLIGATCHYVTADLDQGPIIEQDVIRIDHSDAVEDMVRYGKDIEKTVLARGLRYHL
                     EDRVLVHGNKTIVFR"
     gene            complement(1078..1500)
                     /locus_tag="PKN25_12960"
     CDS             complement(1078..1500)
                     /locus_tag="PKN25_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNV91856.1"
                     /translation="MDTLDHQLIGLLRNDARLSVAALAHKLQVSRGTVTNRIARLEDQ
                     GIIVGYTVRLRPDAQPHDIHAWTSIAVEGNETRAVIASLLGEPAVAELHDTNGRWDLL
                     VGLRAANLTELSTVLERIRLIKGIQSSETSIHLKTYRV"
     gene            1645..2733
                     /locus_tag="PKN25_12965"
     CDS             1645..2733
                     /locus_tag="PKN25_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HNV91857.1"
                     /translation="MSAPEARRLDTRYLSAEHVAGMVRRKGLAECIAGVASYLRQDFL
                     RWNDFDKCARVATHSEVGVIELMPVSDARSYAFKYVNGHPSNHRYQLPTVMAFGVLAD
                     VETGTPELVSELTLTTAIRTAAMSAVAARALAREDVRRMALIGNGAQSEFQALAFHHL
                     LGVEVFHLFDVDPSATRKLAANLERLGLAWRAFGSVRAAVRGCDIVTTATADKTRAAI
                     LTADMIEPGMHLNAVGGDCPGKTELHPDVLRLGPVFCEFEPQSRIEGDLQQMPADFPV
                     TELWQVLAGRIQGRSGAAQVTIFDSVGFALEDYSALRYMRDLALELGMGERLALIPAL
                     DDPKDLFGFIAAEASIAAASGAATLAAC"
     gene            2935..4335
                     /locus_tag="PKN25_12970"
     CDS             2935..4335
                     /locus_tag="PKN25_12970"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNV91858.1"
                     /translation="MISVEKIGGTSMSAFGDVLRHIMLYDKSRILGRIYVVSAYSGVT
                     NQLLEHKKTGERGIYALFAEGSDYHGALDALAVSLKKLNAGFAELALPLDVADAFVDT
                     RIGEVKKYLDAMQHVLASGYIRRQDVLLAAREVLASVGESHSAFNSVEILKANGVNAI
                     LLDLAGFHDDKAWTIDERIHNSFKGLDLDNSVIVATGYTKGTEGIMREFDRGYSEVTF
                     SKIAVEVRPAEAVIHKEFHLSSADPNIVGLENAVIVGATNYDVADQLADVGMEAIHPK
                     AAKPMELAGIPIRLKNTFEPEHPGTLITKDYVGERARVEMVTGSDKVTLVEIHDPSMV
                     GTVGFDLGIMEIFCRHDISYILKATNANSIAHVLWENSVTPALIAELEARYQVVTVKN
                     SAIVCAIGSNISIPGVLARAAQALADAQINVNCVSQTLRQVNMQFIIERSDYKKAIIA
                     LNHSLCVTPGVPVPRA"
     gene            4465..4956
                     /locus_tag="PKN25_12975"
     CDS             4465..4956
                     /locus_tag="PKN25_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNV91859.1"
                     /translation="MPKHSTPAELPVQFEPEFIAGIREIFEQKIAFNALLGLQIDDIG
                     GERVHGRIAMRHELVGHFVHNRIHGGVIAACLDAMGGLAVMAAIGARHLDEPPFQRLH
                     RFSKLGTIDLRIDYLRPGIGESFRMEAEVLRLGSRVASTRMSFLDADGKLLSTGSAAY
                     IVS"
     gene            4964..5326
                     /locus_tag="PKN25_12980"
     CDS             4964..5326
                     /locus_tag="PKN25_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV91860.1"
                     /translation="MSAEIQAAGRGNLFAALPPSAGGEIFQTLFANPRCRVERIVSHG
                     HASAPGEWYDQAGDEWVALLAGAAVLRFEGGDDDGDDVKLKAGDWLVIPAHRRHRVES
                     TSADALWLAVHCAADPAK"
     gene            complement(5388..6095)
                     /locus_tag="PKN25_12985"
     CDS             complement(5388..6095)
                     /locus_tag="PKN25_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV91861.1"
                     /translation="MSASLPRLLTTLAAIGGLAAAGTAHASLINGGFETGTGTPFVAD
                     ANWALFNESAVPGWETTATDNQIEIWGNNFSSVSGGPVPAYEGRQFAEINATQFATLY
                     QDVFGIATGSVVGFEFAHRGRSGVDTMRMMITDLGLDNLLGGGDDTVLFSKNYSTGNT
                     AWAFYTSAGEAPISALGNATRFSYQAISTANGSPSVGNFIDAADFGVGVQAVPEPASI
                     ALIALGLAGLGLRRKRA"
     gene            complement(6456..>7249)
                     /locus_tag="PKN25_12990"
     CDS             complement(6456..>7249)
                     /locus_tag="PKN25_12990"
                     /inference="COORDINATES: protein motif:HMM:NF025389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HNV91862.1"
                     /translation="SWISMLFSDWHSYDHYGLAFVAFFGTLAAVFLIQWVMMRSRWAG
                     WMQSLQGVAPPFMNALGVLFGLVLAFLANDTWSAHDRAMSAVYREADGLRSIGALAAT
                     LPEPLGGELRAAAAGYARASAAEWTQLARRSEAPEVLARADALLALLAAPALAEAAGA
                     NVQALLLRKASDIRSDHDLRVALSQTHVNPLKWLGMAFLGFLTLVSVAMAHLERPRAA
                     FAAVLLFALAAAPTAAIVLIQGNPFQQPSSVTPAPIVAVAKALER"
BASE COUNT         1178 a         2492 c         2452 g         1127 t
ORIGIN      
        1 acgacaccgc gatgctcggc gaagcccacg gcggcatcct cttccacccg cccgagaacg
       61 tgatccgcga gttcccgcag taccccgtgg tgcgcgacta cgccggcctg cgcgccgaga
      121 tcgacaaggt cttcgcgcgc gtctgacccg ctccgctccc cgctccagac ccctcacgcc
      181 tcacgcccag catgcaccgc caacgcttct tcacgctgtc cgcctcctgc cccgaccgcg
      241 tcggcatcgt cgccaaggtc tccggcttca tcgccgagca caagggctgg atcctcgaga
      301 cctcgctgca cgccgagccg ccgctcgagg cgggggaggt cgggcgctac ttcatgcgca
      361 tcgagatcaa ggccgattcg ctgcccttcc atctcgccga gttccgcgag aaattccgcc
      421 cgctcgccga agagctggag atggactgga agatcaccga ctccgcggtg aagaagcgcg
      481 tggtggtgct ggtgagcaag caggagcatt gcctgtacga cctgctcgcg cgctggcagt
      541 cgaaggagct cgacatcgag atcccctgcg tgatttccaa tcacgacacc ttccgcggct
      601 tcgtcgagtg gcacggcatc cctttccacc acgtgccggt cgggaccgac aacaagtccg
      661 cggcgtatgc cgaggtgcgg cgcatcttcg aggaagtgcg cggcgacacc atggtgctgg
      721 cgcgctacat gcagatcctg tcgccggacc tgtgcgcggc ctacccgggc cgcatcctca
      781 acatccacca cagcttcctg cccagcttcg tcggcgccaa gccttaccac caggcgtacg
      841 cgaagggtgt caagctgatc ggcgccacct gccattacgt gaccgccgac ctcgaccagg
      901 gcccgatcat cgagcaggac gtgatccgca tcgaccactc cgacgcggtc gaggacatgg
      961 tgcgctacgg caaggacatc gagaagaccg tgcttgcgcg cggcctgcgc taccacctgg
     1021 aagaccgcgt gctggtgcat gggaacaaga ccatcgtgtt ccgctgatga caggcgccta
     1081 cacccgatag gttttcaggt ggatgctggt ctcgctgctc tggatgccct tgatcaggcg
     1141 gatgcgctcg agcacggtcg atagctcggt gaggttggcc gcgcgcaggc cgacgagcag
     1201 gtcccagcgg ccgttggtgt cgtgcagctc ggcgaccgcg ggctcgccga gcaggctggc
     1261 gatcaccgcg cgtgtctcgt ttccctccac cgcgatgctc gtccatgcgt ggatgtcgtg
     1321 cggctgcgcg tcggggcgca ggcgaacggt gtagccgacg atgatgccct ggtcctccag
     1381 gcgcgcgatg cggttggtga ccgtgccgcg cgagacctgg agcttgtgcg cgagcgcggc
     1441 cacgctcagg cgggcgtcgt tgcgcagcag gccgatcaac tgatggtcca gggtgtccat
     1501 tcgatcattc cggcaattca gcctggcaat tttgcctaaa ggctgacgtt ttcgatacag
     1561 agttgcggat tctgtttggc ggggtattcg gtcaagatgg cgtcatcgca gcgccacatc
     1621 cgataacgag gacggagacc gatcatgagc gcccctgaag ccagacgcct ggacacccgc
     1681 tacctgagcg ccgagcatgt cgccgggatg gtgcgccgca agggcctcgc cgagtgcatc
     1741 gccggcgtcg ccagctacct gcgccaggac ttcctgcgct ggaacgattt cgacaagtgc
     1801 gcccgggtcg ccacgcactc cgaagtcggc gtgatcgagc tgatgccggt gtccgatgcc
     1861 cgcagctacg cgttcaagta tgtgaatggc cacccgtcga accaccgcta ccagctgccg
     1921 acggtgatgg ccttcggcgt gctggccgac gtcgagaccg gcacccccga gctggtcagc
     1981 gagctgaccc tgaccaccgc gatccgcacc gcggcgatgt ccgcggtggc ggcgcgggcg
     2041 ctggcgcgcg aggacgtgcg ccgcatggcg ctgatcggca acggcgcgca gagcgagttc
     2101 caggcgctcg ccttccacca cctgctgggg gtcgaggtct tccacctctt cgacgtcgac
     2161 ccctccgcca cccgcaagct cgccgccaac ctcgagcgcc tgggcctggc ctggcgcgcc
     2221 ttcggcagcg tgcgcgcggc cgtgcgcggc tgcgacatcg tgaccacggc gaccgccgac
     2281 aagacccggg ccgcgatcct caccgccgac atgatcgagc ccggcatgca cctcaacgcc
     2341 gtgggcggcg actgcccggg caagaccgag ctgcaccccg acgtgctgcg cctcggcccg
     2401 gtgttctgcg agttcgagcc gcaatcgcgc atcgagggcg atctgcagca gatgcccgca
     2461 gacttcccgg tgaccgagct gtggcaggtc ctcgccggcc gcatccaggg gcggagcggt
     2521 gccgcccagg tcacgatctt cgactcggtc ggcttcgcgc tggaagacta ctcggcgctg
     2581 cgctacatgc gcgacctcgc gctggagctg ggcatgggcg agcgcctggc gctgatcccg
     2641 gcgctcgacg atcccaagga cctgttcggc ttcatcgccg cggaggcctc catcgcggct
     2701 gcatcgggcg cggcgacgct ggcggcctgc tgagccggtc ggaaggccgc tccgcttcgg
     2761 gcggagccgc ccgcgggcgg cgtgtccgct ggtccggacg agaaaatcac gccgaaatgg
     2821 cgccgaattc gtccgcagct atgtccattt ccgcgccaaa acaataggat gacgggtttc
     2881 ggtcggtctc taatcagaac tggaacacac gtcatgcaaa gctcgggaaa acggatgatc
     2941 tcggttgaaa agatcggtgg cacctcgatg tccgccttcg gggatgtgct gcgccacatc
     3001 atgctctacg acaagtcgcg catcctcggc cgcatctacg tggtgtcggc ctattccggc
     3061 gtgaccaacc agctgctgga gcacaagaag accggcgaac gcggcatcta cgccctgttc
     3121 gcggaaggtt ccgactacca cggcgcgctc gacgcgctcg cggtcagcct caagaagctc
     3181 aacgccggct tcgccgagct cgccctgccg ctggacgtcg ccgacgcctt cgtcgacacc
     3241 cgcatcggcg aggtcaagaa atacctcgac gcgatgcagc atgtgctcgc cagcggctac
     3301 atccgccgcc aggacgtgct gctcgccgcg cgcgaggtgc tggcctcggt gggcgagtcg
     3361 cacagcgcct tcaacagcgt cgagatcctc aaggccaatg gcgtcaacgc catcctgctc
     3421 gacctcgccg gcttccacga cgacaaggcg tggaccatcg acgagcgcat ccacaacagc
     3481 ttcaaggggc tcgacctcga caacagcgtg atcgtcgcca ccggctacac caagggcacc
     3541 gagggcatca tgcgcgagtt cgaccgcggc tactccgagg tcaccttcag caagatcgcg
     3601 gtcgaggtgc gccccgccga ggcggtcatc cacaaggaat tccacctctc ctcggccgat
     3661 cccaacatcg tcggcctcga gaatgcggtg atcgtgggcg cgaccaacta cgacgtcgcc
     3721 gaccagctcg ccgacgtcgg catggaggcg atccacccca aggcggccaa gccgatggag
     3781 ctcgccggca tcccgatccg cctcaagaac accttcgagc ccgaacaccc gggcaccctg
     3841 atcaccaagg actacgtcgg cgagcgcgcc cgcgtcgaga tggtgaccgg cagcgacaag
     3901 gtgaccctgg tcgagatcca cgaccccagc atggtcggca ccgtcggctt cgacctcggg
     3961 atcatggaga tcttctgcag gcacgacatc tcctacatcc tcaaggcgac caacgccaac
     4021 tcgatcgccc acgtgctgtg ggagaactcg gtgacgcccg cgctgatcgc cgagctcgag
     4081 gcgcgctacc aggtggtgac cgtcaagaac agcgcgatcg tgtgcgcgat cggctccaac
     4141 atcagcatcc cgggcgtgct cgcacgcgcg gcgcaggcgc tcgccgacgc gcagatcaac
     4201 gtgaactgcg tgtcgcagac cctgcgccag gtgaacatgc agttcatcat cgagcgcagc
     4261 gactacaaga aggcgatcat cgccctcaac cattcgctgt gcgtgacgcc cggcgtgccg
     4321 gtgccgcgcg cctgatcgtt gcctcgcgcc ctgcggggcg ctgcaggctg cggcgcgtat
     4381 ccgcgcccgc aagcctgccc gtggccccgg tgcgtgcccg ccacgcccgg ggctgcgcca
     4441 tttcttgcaa gggagcatgc atccatgccg aaacactcca ccccggcgga gctgccggtg
     4501 cagttcgagc cggagttcat cgccggcatc cgcgagatct tcgagcagaa gatcgccttc
     4561 aacgcgctgc tcggcctgca gatcgacgac atcggcggcg agcgcgtgca tggccgcatc
     4621 gcgatgcgcc acgagctcgt cggccatttc gtgcataacc gcatccacgg cggcgtgatc
     4681 gccgcctgcc tcgacgccat gggcggtctc gcggtgatgg ccgcgatcgg cgcccgccac
     4741 ctcgacgaac cgcccttcca gcgcctgcac cgcttctcca agctcggcac catcgacctg
     4801 cgcatcgact acctgcgccc cggcatcggc gaatccttcc gcatggaagc cgaggtcctg
     4861 cgcctgggtt cgcgcgtcgc gtcgacgcgg atgagcttcc tcgacgccga cggcaagctg
     4921 ctgtccaccg gttcggcggc gtacatcgtt tcgtgagcgg ggaatgagcg ccgagatcca
     4981 ggctgccggg cgcggcaatc tcttcgccgc gctgccgccg tccgcaggcg gcgagatctt
     5041 ccagaccctg ttcgccaatc cgcgctgccg tgtcgagcgc atcgtctcgc acggccatgc
     5101 gagcgcgccc ggcgagtggt acgaccaggc tggcgacgag tgggtggcgc tgctggcggg
     5161 cgcggcggtg ctgcgcttcg aggggggcga cgatgacggc gacgacgtga agctgaaggc
     5221 cggtgactgg ctcgtgatcc cggcgcatcg gcgtcaccgc gtcgagtcga cctcggcgga
     5281 tgcgctgtgg ctggcggtgc actgcgcagc cgaccccgca aagtgaaacg gccccgggcg
     5341 aaccgccggg gccgtggtgt caagccgcct gcactgcgac gcgtgtctca ggcgcgcttg
     5401 cggcgcagcc ccagccccgc gaggccgagc gcgatcagcg cgatcgacgc cggttccggc
     5461 accgcctgca cgccgacgcc gaagtcggcg gcgtcgatga agttgccgac cgaggggctg
     5521 ccattggcgg tgctgatggc ctgatacgag aagcgcgtgg cattgccgag ggcgctgatc
     5581 ggcgcctcgc cggcgctcgt gtagaaggcc caggcggtgt tgccggtcga gtagttcttg
     5641 gagaacaaca ccgtgtcatc gccaccgccg agcaggttgt cgaggccgag gtcggtgatc
     5701 atcatgcgca tcgtgtccac ccccgaacgt ccgcggtggg cgaactcgaa gcccacgacg
     5761 ctgcccgtgg cgatgccgaa cacgtcctgg tacagggtgg cgaactgggt ggcgttgatc
     5821 tcggcgaact ggcgcccctc gtaggcgggc accgggccgc cgctcaccga gctgaagttg
     5881 ttgccccaga tctcgatctg gttgtcggtg gcggtggttt cccagccggg taccgccgac
     5941 tcgttgaaca gcgcccagtt ggcgtcggcg acgaagggcg tccccgttcc ggtttcgaag
     6001 ccgccgttga tcaggctggc gtgggcggtg cccgccgcgg ccagaccgcc gatcgcggcg
     6061 agggtggtga ggaggcgggg gagcgaggcg gacatgggtg gttcctttat ttttgggcga
     6121 cacgttcggt gacgggcgtc gacgcgaaag gtcggcccga gagtttcgac cagatcttaa
     6181 gcaatgatcg cgccagcacg cgcggtagaa ggtcacagcg cgttgattcc agtgggaaat
     6241 gggcctctgg agaggaatgc acgggccatc ccgggcgtgc tcgagttgcc gcagtgtcaa
     6301 tttttccgac agccatcgac cgggggcggc cgcgtccatc gcgacggatt cgccgtttgc
     6361 ccgatcgggt gtcgcaaggc gcagcagggc ctgcagcgcg agggcgcgcg gcgggacctc
     6421 ggcacggcgc tgcaaccgca gggccgaccg gcgcttcagc gctccagcgc cttggcgacc
     6481 gcgacgatcg gcgcgggcgt gaccgagctc ggctgctgga aggggttgcc ctggatcagc
     6541 acgatggcgg cggtgggggc cgcggcgagc gcgaacagca gcaccgccgc gaaggccgcg
     6601 cgcgggcgtt cgaggtgggc catggccacc gagaccaggg tcaggaagcc caggaaggcc
     6661 attcccagcc acttcagcgg gttgacgtgg gtctggctga gtgcgacgcg caggtcgtgg
     6721 tcggaacgga tgtcgctcgc cttgcgcagc agcagggcct gcacgttggc gcccgcggcc
     6781 tcggcgagcg cgggggcggc gagcagcgcc agcagcgcgt cggcgcgggc gagcacctcc
     6841 ggcgcctcgc tgcggcgcgc gagctgggtc cattcggcgg cgctggcgcg ggcgtagccg
     6901 gctgccgccg cgcgcagctc gccgccgagc ggctcgggca gcgtggcggc gagcgcgccg
     6961 atgctgcgca ggccgtccgc ctcgcgatac accgcgctca tcgcgcggtc gtgcgcgctc
     7021 caggtgtcgt tggcgagaaa ggccagcacc aggccgaaga gcacgccgag cgcgttcatg
     7081 aacgggggcg ccaccccctg cagggactgc atccagcccg cccagcgcga gcgcatcatc
     7141 acccactgga tcaggaacac ggcggcgagg gtgccgaaga aggcgacgaa ggcgaggccg
     7201 tagtggtcgt agctgtgcca gtcggagaaa agcatggaga tccaggagg
//
LOCUS       DAOQBS010000139         7166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_10204_length_7166_cov_12.393053, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000139 DAOQBS010000000
VERSION     DAOQBS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10204_length_7166_cov_12.393053"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..397
                     /locus_tag="PKN25_12995"
     CDS             77..397
                     /locus_tag="PKN25_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNV91863.1"
                     /translation="MALTRDFKQTVAARVQRDPAFAQALLDEAITLFINGEPDTAKLV
                     LRDLVNATLGFESLAEEIHKPAKSLHRMLSASGNPTMSNISAIFAAIKRALGVEVRTT
                     VVVA"
     gene            complement(460..1173)
                     /gene="torD"
                     /locus_tag="PKN25_13000"
     CDS             complement(460..1173)
                     /gene="torD"
                     /locus_tag="PKN25_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HNV91864.1"
                     /translation="MDARSQAGTPPPLSPAEWRFASTQRAGLYGWFSRLYAQEVTDEL
                     FRSHFADGGFAPFAGLAELGLAAEVQRLDDAVAALRAEQLPRLELAADFAQLFLLDGT
                     TSALPYASAYAGKDAASPLYGAAEARMREFLTARKLAIQADFREPADHLAVALAVMAE
                     VAEKDSGASDLPTAAATQADFLRTALLDWLPHFVARCQQARPRFDVYPALAALLLGFV
                     RADMAFLGDVGGGAGAAAD"
     gene            complement(1173..3689)
                     /gene="torA"
                     /locus_tag="PKN25_13005"
     CDS             complement(1173..3689)
                     /gene="torA"
                     /locus_tag="PKN25_13005"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HNV91865.1"
                     /translation="MNYSRRGFLKAMAAMSGSALVAPGLLTAAPALAAGEAAAVGDDI
                     TTWKISGSHFGAIRAKVVGDRVVDIRPFEHDKHPTEMVKGLLGLIYSPSRVRYPMVRL
                     DWYRKRHLSDTSQRGDNRFVRVSWDEALDLFYEELERIQKDYGPWALHTGGVGWRSVG
                     QVHSCGNHMVRGVGMHGRSVGTIGDYSTGAGQMILPYVLGSTEVYSQGTSWDVILKES
                     KLVVFWANDPIKNLQVGWNTETHESYAYFEQLKAKVADKSIQVICIDPVKSKTLNYLG
                     GAEHQYINPMTDVPLMLAIAHTLVKEGLHDQKFLDTYTLGFNQFLPYLQGKTEDKVEK
                     TPEWAEKICGVPSDRIRELARLMARHRTQLIFGWAVQRQQHGEQPYWMGAVLAAMLGQ
                     IGLPGGGISYAHHYSSVGVSSSGAAMPGAFPLNLDPGRKPRHDNTDYKGYSAVVPIAR
                     VIDALLEPGKEIDFNGGKVKLPPYKMAIFSGCNQWHRQPQRNRMKEAWQKLETVVAID
                     YNWTATCRFADIVLPACTPFERNDLDGYGSYSNRGILAMQKLIDPLFHSRPDFEIFRG
                     LTRRFNRDAEYTRGMDEMQWIEKIYEDCRKENGLKDIAMPPFAEFWQKGYVLFPEGKP
                     WVRHADFRDDAEVNALGTPSGFIEIFSRKIERYGYADCKGHPVWMEKTERSHGGPGSD
                     RFPLWLQSVHPDKRLHSQLCESEPLRETYAIAGREPVFIGPEDAAARGIAHGDLVRVF
                     NDRGQLLAGAHVSDNFPRGVVRIQEGAWYGPTGPEIGALDTYGDPNTLTLDIGTSSLA
                     QATSANTCLVQIEKFVGTPPRVTSFGGPIEVDPEGREVKA"
     gene            complement(3706..4929)
                     /gene="torC"
                     /locus_tag="PKN25_13010"
     CDS             complement(3706..4929)
                     /gene="torC"
                     /locus_tag="PKN25_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HNV91866.1"
                     /translation="MKNLLRSLRDLFLKPSVRIGLGVLVTGGFIAGVLAWQGFNTAME
                     ATNKEEFCLSCHTMHDNLLPELQKTVHWNNRSGVRARCPDCHVPHDFTDKVARKMQAS
                     REVLGQLLGTIDTREKFKEHRIVLAQREWKRFAANGSKECRACHDYKDMNFDKMRPAS
                     QVAMRKAAERNQSCVDCHKGVAHQLPEMKGARNPAFDTLVSSASGQSVATGKDYFLVV
                     PQELFADEGLSKRIGSVEVATQVKVLETKGDAQRVEFALWRKNKGYGRVWYSHFGLNI
                     TSATFDKDVAQDTSFVKVLDTREDPMTGLEWQQVTATAWMRKGSVLDSVEPVWSIARA
                     SYSSSCSVCHRQPDEAHFDANTWPGLFGGMVGFTSMDDDTARVVLKYLQTHSSDFSTS
                     HGAGSPDSALQTARP"
     gene            complement(5005..5190)
                     /locus_tag="PKN25_13015"
     CDS             complement(5005..5190)
                     /locus_tag="PKN25_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91867.1"
                     /translation="MNNQASGHSAPSGAQAHGRPGEWRRFLLLAFVGLPITMVLAIAA
                     YGFVVWFLQILFFGPPS"
     gene            complement(5385..>5786)
                     /locus_tag="PKN25_13020"
     CDS             complement(5385..>5786)
                     /locus_tag="PKN25_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91868.1"
                     /translation="PLVDRLLAGVPRTVEVTPERAGALWAARRGLFFKPFAGFGSRAT
                     YRGDKLTQRVWQEILAGGYVAQDLALPSARRVAVDGQSSDLKLDVRAYAYAGAIRLVA
                     ARLYKGQTTNFRTPGGGFAPVFVATAGRLSG"
     assembly_gap    5787..5886
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5887..6489)
                     /locus_tag="PKN25_13025"
     CDS             complement(<5887..6489)
                     /locus_tag="PKN25_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91869.1"
                     /translation="MTMETSDFSFSLHAGARDMAEAINRGCDCRSLDPERLRRQLEAE
                     PSLLGLAAEIGRSRPQLFSATAVFIAPATLAAMADLIGAVESVLTLPACQAIALDRAP
                     PIARIALGPKSVFMGFDFHLGEAGPQLIEINTNAGGALLNGALARAQKACCEQIQPFL
                     RPTAALGTLDQRWLDDFLADWSLQGRSGKPARVAIVDEHPT"
     gene            6712..>7166
                     /locus_tag="PKN25_13030"
     CDS             6712..>7166
                     /locus_tag="PKN25_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNV91870.1"
                     /translation="MQTENLSAWTHDHVFDTGNALAERSTRVVMWITAAAMVIEITAG
                     WWFNSMALLADGWHMSSHAFAIGVSALAYAAARRYAQDRRFAFGTWKIEILGGFASAI
                     FLLGVAAMMVVGSIERIVSPQAIQYREAIAVAILGLAVNLVCALILGRA"
BASE COUNT         1060 a         2281 c         2520 g         1205 t
ORIGIN      
        1 gcgatatcga cgaagcggtg gccttgtggg aggactacaa gcgccgcaag gcccggatga
       61 ataaaggagc ctgaccatgg cactgacccg cgacttcaag cagaccgtcg ctgcacgggt
      121 gcagcgcgac cccgcgttcg cgcaagcgct gctcgacgag gcgatcaccc tgttcatcaa
      181 cggtgagccc gacaccgcaa agctggtttt gcgagacctt gtgaacgcga cccttggctt
      241 cgagtccctc gccgaggaga tccacaagcc cgcgaaaagc ctgcaccgga tgctgtcggc
      301 gtcgggcaat ccgacgatga gcaacatctc ggcgatcttt gccgccatca agcgggcgct
      361 cggggtcgaa gtgcggacga ccgtcgtcgt ggcatgagtg cgggcgcgca ccgagacgtg
      421 cgctcgcacc gtcgggtgtg acggtgcagg gcgcgcgcgt cagtctgcgg ctgcccccgc
      481 acccccgccc acatccccca gaaacgccat gtccgcacgc acgaacccga gcagcagggc
      541 cgccagcgcc gggtagacgt cgaagcgcgg ccgcgcctgc tggcagcggg cgacgaagtg
      601 cggcagccag tccagcagcg cggtgcgcag gaaatccgcc tgggtcgcgg ctgcggtggg
      661 gaggtcgctg gcccccgagt ccttctccgc cacctccgcc atcaccgcca gcgccacggc
      721 gaggtggtcg gcgggttcgc ggaagtcggc ctggatcgcc agcttgcgcg cggtgaggaa
      781 ctcgcgcatg cgcgcctccg ccgcgccgta gagcggcgag gcggcgtcct tgcccgcgta
      841 ggccgaggcg tagggcaggg cgctggtcgt gccgtcgagc aggaagagct gggcgaagtc
      901 cgcggccagc tccaggcgtg gcagttgctc cgcgcgcagc gcggcgacgg cgtcgtcgag
      961 gcgctgcacc tcggcggcga ggcccaactc ggccagcccc gcgaaggggg cgaagccccc
     1021 gtccgcgaag tggctgcgga acagctcgtc ggtgacctcc tgcgcgtaca gccgggagaa
     1081 ccagccgtac agcccggcgc gctgcgtgct cgcgaaccgc cattccgcgg gcgagagggg
     1141 cgggggtgtt cctgcctgcg atcgcgcgtc catcacgcct tcacctcccg gccttccgga
     1201 tcgacctcga tcgggccgcc gaaggacgtc acccgcggcg gggtgccgac gaacttctcg
     1261 atctgcacca ggcaggtgtt ggcgctggtg gcctgcgcca ggctggaggt gccgatgtcg
     1321 agcgtcagcg tgttggggtc gccgtaggtg tcgagcgcgc cgatctccgg accggtcggt
     1381 ccgtaccagg cgccttcctg gatgcgcacc acgccgcgcg ggaagttgtc cgacacgtgc
     1441 gcgccggcca gcaactggcc gcggtcgttg aagacgcgca ccaggtcgcc gtgggcgatg
     1501 ccgcgcgcgg cggcgtcctc ggggccgatg aacaccggct cgcggccggc gatggcgtag
     1561 gtctcgcgca gcggctcgga ttcgcacagc tgcgagtgca ggcgcttgtc cgggtgcacc
     1621 gactgcagcc acagcgggaa gcggtcggag ccggggccgc cgtgcgagcg ctcggtcttc
     1681 tccatccaca ccgggtggcc cttgcagtcg gcgtagccgt agcgctcgat cttgcggctg
     1741 aagatctcga tgaagcccga cggcgtgccg agcgcgttga cctcggcgtc gtcgcggaag
     1801 tcggcgtggc gcacccaggg cttgccctcg gggaacagca cgtaaccctt ctgccagaac
     1861 tcggcgaacg gcggcatggc gatgtccttg aggccgttct ccttgcggca gtcctcgtag
     1921 atcttctcga tccactgcat ctcgtccatg ccgcgggtgt attcggcgtc gcggttgaag
     1981 cgccgggtga ggccgcggaa gatctcgaag tcggggcgcg agtggaagag cgggtcgatg
     2041 agcttttgca tcgcgaggat gccgcggttg ctgtacgagc cgtagccgtc gaggtcgttg
     2101 cgctcgaagg gcgtgcacgc cggcagcacg atgtcggcga agcggcaggt ggcggtccag
     2161 ttgtagtcga tggcgaccac ggtctcgagc ttctgccagg cttccttcat gcggttgcgc
     2221 tggggctggc gatgccactg gttgcagccg ctgaagatcg ccatcttgta gggcggcagc
     2281 ttgaccttgc cgccgttgaa gtcgatctcc ttgcccggct cgagcagggc gtcgatgacg
     2341 cgcgcgatcg gcaccaccgc gctgtagccc ttgtagtcgg tgttgtcgtg cctgggcttg
     2401 cggccggggt cgaggttgag cgggaaggcg ccgggcatgg cggcgcccga cgaggacacg
     2461 cccaccgagc tgtagtggtg cgcgtagctg atgccgccgc cgggcaggcc gatctggccg
     2521 agcatggcgg cgagcaccgc gcccatccag tagggctgct cgccgtgctg ctggcgctgc
     2581 accgcccagc cgaagatgag ctgggtgcgg tgcctggcca tcaggcgggc gagctcgcgg
     2641 atgcgatcag aaggcacgcc gcagatcttc tccgcccact cgggcgtctt ctcgaccttg
     2701 tcctcggtct tgccttggag gtagggcagg aactggttga agccgagggt gtaggtgtcg
     2761 aggaacttct ggtcgtgcag gccttccttc accagcgtgt gcgcgatggc gagcatcagc
     2821 ggcacgtcgg tcatcgggtt gatgtactgg tgctcggcgc cgccgaggta gttgagcgtc
     2881 ttgctcttga ccgggtcgat gcagatcacc tggatcgact tgtcggcaac cttggccttg
     2941 agctgctcga agtaggcgta ggactcgtgg gtctcggtgt tccagcccac ctgcaggttc
     3001 ttgatcgggt cgttggccca gaacacgacg agcttgctct ccttgaggat cacgtcccac
     3061 gaggtacctt gcgaatacac ctcggtcgag ccgagcacgt agggcaggat catctgcccg
     3121 gcgccggtcg agtagtcgcc gatggtaccg acgctgcggc cgtgcatgcc gacgccgcgc
     3181 accatgtggt tgccgcagct atgcacctgg ccgaccgagc gccagcccac gccgccggtg
     3241 tgcagcgccc acgggccgta gtccttctgg atgcgctcga gttcttcgta gaacaggtcg
     3301 agcgcctcgt cccagctcac gcgcacgaag cggttgtcgc cgcgctggct ggtgtcgctg
     3361 aggtggcgct tgcggtacca gtccaggcgc accatcgggt agcgcacgcg ggacgggctg
     3421 tagatcaggc cgagcaggcc cttgaccatc tccgtcggat gcttgtcgtg ctcgaagggg
     3481 cggatatcga ccacgcggtc gccgaccacc ttggcgcgga tggcgccgaa gtgcgagccg
     3541 gagatcttcc aggtggtgat gtcgtcgccg accgcggccg cctcgccggc cgcgagggcc
     3601 ggggcggcgg tgagcagacc gggcgcgacc agcgcgctgc ccgacatcgc cgccatggcc
     3661 ttgaggaaac ctcgccgaga gtagttcatg gtgttcaact cctgatcagg gacgcgcggt
     3721 ctgcagggcg gaatcgggcg aaccggcgcc atgcgacgtg ctgaaatcgg acgaatgggt
     3781 ctggaggtac ttgagcacca cgcgggcggt gtcgtcgtcc atgctggtga agccgaccat
     3841 gccgccgaac aggcccggcc aggtgttggc gtcgaagtgc gcttcgtcgg gctggcggtg
     3901 gcacacgctg cagctgctgc tgtagctggc gcgggcgatc gaccacaccg gttcgacgct
     3961 gtcgagcacg ctgcccttgc gcatccacgc ggtggcggtg acctgctgcc attcgagacc
     4021 ggtcatcggg tcttcccggg tgtcgagcac cttcacgaag ctcgtgtcct gcgcgacgtc
     4081 cttgtcgaag gtggcgctgg tgatgttgag gccgaagtgg ctgtaccaga cgcgaccata
     4141 gcccttgttc ttgcgccaca gcgcgaactc gacgcgctgc gcgtcgccct tggtctcgag
     4201 caccttgacc tgggtggcga cctcgaccga gccgatgcgc ttgctcagac cttcgtcggc
     4261 gaagagttcc tgcggcacca ccaggaagta gtccttgccc gtagccacgc tctgtccgga
     4321 ggcggaggac accagggtgt cgaaggccgg gttgcgcgcg cccttcatct cgggcaactg
     4381 gtgggcgacg cccttgtggc agtcgacgca gctctggttg cgctcggcgg ccttgcgcat
     4441 cgccacctgc gatgccggac gcatcttgtc gaagttcatg tccttgtagt cgtggcaggc
     4501 acggcattcc ttcgagccgt tggccgcgaa gcgcttccac tcgcgctgcg ccagcacgat
     4561 gcggtgctcc ttgaacttct cgcgggtgtc gatggtgccg aggagctggc cgagcacctc
     4621 gcggctggcc tgcatcttgc gcgccacctt gtcggtgaag tcgtgcggca cgtggcagtc
     4681 cgggcagcgc gcgcgcacgc cgctgcggtt gttccagtgc accgtcttct gcagctcggg
     4741 cagcaggttg tcgtgcatgg tgtggcagct caggcagaac tcttccttgt tggtggcttc
     4801 catggcggtg ttgaagcctt gccaggccag cacgccggcg atgaagccgc cggtgaccag
     4861 cacgccgagg ccgatccgaa ccgatggctt gaggaagagg tcccgcaagg acctgagtag
     4921 attcttcatt tcaatgctcc tgcttgttgt ccggttcccg ggcctgcggc cctgccgcga
     4981 ggcctgtgtt gcagcgtgga gggatcagga cggggggccg aagaacagga tctgcaggaa
     5041 ccagaccacg aatccgtagg ccgcgatcgc cagcaccatg gtgatgggca ggccgacgaa
     5101 ggcgagcagc aggaatctgc gccactcgcc cgggcgtccg tgcgcctggg cgccggatgg
     5161 ggcggaatgc ccgcttgctt gattgttcat gtgtcgatct ccgcgttgat gcgttctccg
     5221 gacgaggccg gaggcgacgc gcgcgtggcg cgggatcggc tcgggctggg gatggtccgg
     5281 tactaaagcc acaccccgat catagatatc gggtgttaac gccgagggga aatttgtgaa
     5341 tggaaaggag cgctggtgtt aatggcatta acacaagcct cttttcaccc ggaaagccgc
     5401 ccggcggtgg ccacgaatac cggcgcaaag ccgccgcccg gcgtgcggaa gttggtcgtc
     5461 tgtcccttgt acagccgcgc agcgaccagg cggatcgcgc cggcatacgc atacgcgcgg
     5521 acatcgagct tgagatcgct gctttggcca tcaaccgcga cgcgccgtgc ggagggaagg
     5581 gcgaggtctt gcgcgacgta gccgccggcg agaatctcct gccagacgcg ctgcgtgagc
     5641 ttgtctcccc gataggtcgc acgactgccg aaaccggcga agggtttgaa gaacaggccg
     5701 cgccgtgctg cccacaatgc tcctgcgcgt tccggggtga cttcgaccgt gcgcgggacc
     5761 cctgcgagca ggcggtcgac aaggggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnncgtg ggatgttcgt cgacgatggc gacgcgggcg ggcttgccgc ttcgcccctg
     5941 cagcgaccag tcggcgagga agtcgtccag ccagcgctgg tcgagcgtcc cgagcgctgc
     6001 ggtcgggcgc aggaagggct ggatctgctc gcagcaggct ttttgcgcgc gtgccagtgc
     6061 cccgttgagc agggctccgc cggcgttggt gttgatctcg atgagctgcg ggccggcttc
     6121 gcccaggtgg aagtcgaaac ccatgaacac cgatttcggt ccgagcgcga tgcgggcgat
     6181 cggcggtgcg cgatcgagcg cgatcgcctg acaggccggc agcgtcagga ccgattcgac
     6241 cgcgccgatg aggtccgcca tggccgccag ggttgcgggc gcgatgaaca cggcggtcgc
     6301 ggagaagagc tgcggccggc tgcggccgat ctcggcggcg agcccgagca ggctgggctc
     6361 cgcctcgagc tgccggcgca ggcgctcggg atcgaggctg cggcagtcgc agccccggtt
     6421 gatcgcctcg gccatgtcgc gcgcgccggc gtgcaggctg aagctgaagt cgctcgtctc
     6481 catggtcatc cctttctgca agcccgtccg ggctgttgca tgcccattgg aacacgaccc
     6541 gggtggcccc ggtccccggc ttcggatacc gtacccccta tcggccgagg tcgtcgagga
     6601 gcacgtccgc acccgcatcg ccgatccggc gatgaccgat gatgccgagc gcaccaaggg
     6661 cgccgacgaa ctgatcggcg tcctgcgcac ctatctgaag cgagccccgc catgcaaacc
     6721 gagaacctgt ccgcgtggac ccacgaccac gtcttcgata ccggcaacgc gctcgccgag
     6781 cgcagcacgc gggtggtgat gtggatcacc gccgcggcga tggtgatcga gatcaccgcc
     6841 ggctggtggt tcaactcgat ggcgctgctg gccgatggct ggcacatgag ctcgcacgcc
     6901 ttcgcgatcg gtgtttccgc gctggcctat gccgccgcgc ggcgctacgc gcaggaccgg
     6961 cgcttcgcct tcggcacgtg gaagatcgag atcctgggcg gcttcgccag cgccatcttc
     7021 ctgctcggcg tggcggcgat gatggtcgtc ggctcgatcg agcgcatcgt gtcgccgcaa
     7081 gccatccagt accgcgaggc gatcgcggtc gccatcctgg ggctggcggt caacctcgtg
     7141 tgcgcgctga tcctgggcag ggcgca
//
LOCUS       DAOQBS010000140         7118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_10305_length_7118_cov_13.586436, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000140 DAOQBS010000000
VERSION     DAOQBS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10305_length_7118_cov_13.586436"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PKN25_13035"
     CDS             <1..886
                     /locus_tag="PKN25_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV91871.1"
                     /translation="PYERPVNTVFQSYALFPHMTVSDNIGFGLRRDSAAVRNARVDEL
                     LALVGLAGQGRKYPHEMSGGQQQRVALARALAPKPSLLLLDEPFSNLDVELRERLSYE
                     VREIIKAADTTAILVTHDQHEAFAVADEIGIMHEGRIQQWDTPYNLYHRPANRFVADF
                     IGQGAFLRGKVLDDHRVQIELGVLDSTQPLVCCADCAGCVHGSPVDVLLRPDDLVHDD
                     ASALRAEVVHKAFRGADILYTLRLAGGARVLSLVPSHHNHAIGERIGIRLDVDHVVTF
                     SDERADECAPAHPAASLA"
     gene            883..1590
                     /gene="aat"
                     /locus_tag="PKN25_13040"
     CDS             883..1590
                     /gene="aat"
                     /locus_tag="PKN25_13040"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNV91872.1"
                     /translation="MIPWLGAEPGFPADERALREPNGLLAAGGALTPPWLLAAYRRGI
                     FPWFSEGEPILWWTPDPRLVLVPDEFHVSHSLRKTLRRRRFEVRTDTAFAEVLLACAA
                     ARAGGPGTWITPAMRAAYQRMHELGWAHSVECWREGRLVGGLYGMAIGRVFFGESMFA
                     RETDASKVALAHLARLLAERGYGMIDCQMTTAHLCSLGAREVPRARFTAALRQWAHDG
                     PAPQRWAERTMQDFDWN"
     gene            1621..2349
                     /locus_tag="PKN25_13045"
     CDS             1621..2349
                     /locus_tag="PKN25_13045"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNV91873.1"
                     /translation="MKSDYPYALIQFYATAPYACSYLAGRSARSQVATPGHLIDPQVY
                     SELVRRGFRRSGVFTYRPYCDHCQACVPVRVPVERFQPDRSQRRTWARHADLIPIERP
                     LEFVEEHYQLYQRYQTARHAGGGMDLDNREQYAHFLLQSHVDTRLVEFRSYGSLRMVS
                     VIDRLTDGLSSVYTFYDPDQPRSAFGTWGVLWQIEACRVLGLPHVYLGYWIGESPKMA
                     YKARFRPLEALIDGDWQPFPGDFA"
     gene            2392..3399
                     /locus_tag="PKN25_13050"
     CDS             2392..3399
                     /locus_tag="PKN25_13050"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNV91874.1"
                     /translation="MLYELARPLLFALDPETAHNLTLHGLHYAGKLLPAAEPEPASAV
                     EVMGLRFPNRVGLAAGLDKNGEAIDGLARLGFGFLEIGTITPRPQPGNPRPRMFRLPE
                     VRGIINRMGFNNHGVDVLLAHVRAAKYRGILGINIGKNFDTPIERAADDYLACLEKVY
                     ALASYVTVNISSPNTKNLRQLQGESELDDLLGRLKAAQTRLTEQHGRYVPLTLKIAPD
                     LDDAQVSNIADALRRHRIDGVIATNTTIARDKVQGIAHGNEQGGLSGAPVFEASTAVV
                     RKLAHALAGELPIIAAGGVLEGAQARAKLEAGAALVQLYSGLIYRGPALVRECVRATA
                     G"
     gene            3497..4585
                     /locus_tag="PKN25_13055"
     CDS             3497..4585
                     /locus_tag="PKN25_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV91875.1"
                     /translation="MTHRRQFVLSAAALAVAATLPFAAHAADAAKVGFVYVSPIGDAG
                     WTYQHDLGRKELEKTLGGRIETRYVENVAEGADAERVIREFAASGSKIVFATSFGYMN
                     YVERVARQFPNVTFLHATGYKSAKNFAPYNARFYEGRYLNGVIAGKMTKSNVLGYVGA
                     FPIPEVLQGINAFTLGARSVNPNVEVRVIWANSWYDPGKEREAAMTLISQGADMLTHH
                     TDSTATVQAAEEKGVYAFGYHSDMSKYAPKSQLTATTHHWGEFYARTVDAVLGGTWKP
                     EGVWGGMKDGMIRLAPLNPAIPADVQAMVKELQGRITAGSLHPFTGPVLDQAGKERLA
                     AGAVMDDATLGKMDYFVQGVGSRLPTAK"
     gene            complement(4662..5351)
                     /locus_tag="PKN25_13060"
     CDS             complement(4662..5351)
                     /locus_tag="PKN25_13060"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV91876.1"
                     /translation="MAVDVGHTLDAPGATSARGRSEFEFNRDLSVHVVDALHARGWAV
                     STINADGRIASLRARPQAAGEAGAAFLLSLHHDSVNAVELRPWTWEGRALDYNDEFAG
                     HSLFVSRDNPDTAMSILCARAIGARLQRMGFEPTHKNGRRRSYADREHAVHHYDGLAV
                     LRHARMPAVLFEAGILKNRDEELLLRDPERQARMADAIATGISACLRNGLPADDEAWA
                     DRPDRADPPTP"
     gene            5674..6099
                     /locus_tag="PKN25_13065"
     CDS             5674..6099
                     /locus_tag="PKN25_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNV91877.1"
                     /translation="MSGIFTKSMARNIFYGGTTFFFLLFLALTFDTERELPNRDNRAA
                     ITPEVAAGKHIWETRNCLGCHTLLGEGAYFAPELGNVYKRRGGDFIKGWMQAMPTGAP
                     GRRQMPQFNLTDEELDQLVAFLKYTSEINTANWPPNIEG"
     gene            6131..>7118
                     /locus_tag="PKN25_13070"
     CDS             6131..>7118
                     /locus_tag="PKN25_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNV91878.1"
                     /translation="MQYKSQAVAMPYFIAAIGLFVGQIIFGLILGLQYVVGDFLFPEI
                     PFNVARMVHTNLLIVWLLFGFMGAAYYLIPEETETELFSPKLALAMFWIFLVAGALTI
                     VGYLLVPYSTLASMTGNDILATMGREFLEQPLLTKIGIVIVALAFLFNITMTVLKGRK
                     TVISIVLLLGLWGLAIFFLFSFYNPVNLVRDKFYWWWVVHLWVEGVWELIMAAMLAFV
                     LIKVTGVDREVIEKWLYVIITLALVTGIIGTGHHYFWIGTPEYWQWWGSIFSALEPIP
                     FFAMTVFAFNMVNRRRREHPNKAAVLWALGTGVMAFLGAGVWGFLHTLAPVNY"
BASE COUNT         1152 a         2612 c         2220 g         1134 t
ORIGIN      
        1 tccctacgag cgtccggtca acacggtttt ccagtcctat gcgctcttcc cgcatatgac
       61 ggtgtccgac aatatcggct tcggcctccg gcgcgacagc gccgccgtgc gcaacgcgcg
      121 ggtggacgag ctgctcgcgc tggtcggcct cgccggacag ggccgcaagt atccgcacga
      181 gatgtccggc ggccagcagc agcgcgtcgc gctcgcgcgt gcactcgctc ccaagcccag
      241 cctgctgctg ctcgacgagc ccttctccaa tctcgacgtc gaattgcgcg aacgcctgtc
      301 gtacgaggtg cgcgagatca tcaaggccgc cgacaccacc gcgatcctgg tcacccacga
      361 ccagcacgag gccttcgcag tggccgacga gatcggcatc atgcacgagg gtcgcatcca
      421 gcaatgggat acgccctaca acctctacca ccgcccggcc aaccgcttcg tcgccgactt
      481 catcggccag ggtgccttcc tgcgcggcaa ggtgctcgac gatcaccgcg tgcagatcga
      541 gctcggcgtg ctcgacagca cgcagccgct ggtgtgctgc gccgactgcg ccggttgcgt
      601 gcatggcagc ccggtcgacg tgctgctgcg cccagacgac ctcgtgcacg acgacgccag
      661 cgcgctgcgc gccgaggtgg tgcataaggc tttccgcggc gcagacatcc tgtacaccct
      721 gcgcctggcg ggcggcgcgc gcgtgctctc gctggtgccc agccaccaca accacgccat
      781 cggcgagcgc atcggcatcc gcctcgacgt cgaccacgtc gtcaccttct ccgacgagcg
      841 cgccgacgag tgcgcgcccg cccatcccgc agcgtctctg gcatgatccc ctggctgggc
      901 gcggagccgg gcttccccgc cgacgagcgc gctttgcgcg aacccaacgg gctgctcgcc
      961 gccggtggcg cgctcacccc accctggctg ctcgccgcct accgccgcgg catcttcccc
     1021 tggttcagcg agggcgaacc catcctgtgg tggacacccg accctcgcct ggtgctcgtg
     1081 cccgacgaat tccacgtcag ccattcgctg cgcaagaccc tgcgccgacg gcgcttcgag
     1141 gtcaggacgg acaccgcctt cgccgaggtg ctgctggcct gcgccgcggc gcgcgccggc
     1201 ggcccgggca cctggatcac ccccgcgatg cgcgccgcct accagcgcat gcacgaactg
     1261 ggctgggcgc acagtgtcga atgctggcga gagggccggc tggtgggcgg actctacggc
     1321 atggcgatcg gccgcgtgtt cttcggcgag tcgatgttcg cgcgcgagac cgacgcctcc
     1381 aaggtggcgc tcgcccatct cgcgcggctg ctcgccgaac gcggctatgg catgatcgac
     1441 tgccagatga ccaccgccca cctgtgctcg ctcggggcac gcgaggtgcc gagggcgcgt
     1501 ttcaccgccg ccctgcgcca gtgggcgcac gacggcccgg cgccgcagcg ctgggccgag
     1561 cgaacaatgc aggacttcga ctggaactga cgcaggacgg caagttgcac gacggacgcg
     1621 atgaaatcgg actatcccta cgccctcatc cagttctacg ccacggcgcc ctacgcatgc
     1681 tcctacctgg ccgggcggag cgcgcgctcg caggtcgcca ccccgggcca tctcatcgac
     1741 ccgcaggtct atagcgaact cgtgcgccgc ggctttcgcc gcagcggggt gttcacctac
     1801 cgcccctatt gcgaccactg ccaggcctgc gtgccggtgc gcgtgccggt cgagcgcttc
     1861 cagcccgacc gcagccagcg caggacatgg gctcgacatg cagatctgat cccgatcgag
     1921 cgtcccctcg aattcgtcga ggagcactac cagctctacc agcgctacca gaccgcccgc
     1981 cacgccggcg gcggcatgga cctcgacaac cgcgagcagt acgcccattt cctgctgcag
     2041 agccatgtcg acacccgcct ggtggagttc cgcagctatg gcagcctgcg catggtgagc
     2101 gtgatcgatc gcctcaccga cggcctgtcg agcgtctaca ccttctacga tcccgaccag
     2161 ccgcgcagcg ccttcggcac ttggggcgtg ctctggcaga tcgaggcgtg cagggtgctc
     2221 ggactgccgc acgtgtacct gggctactgg atcggcgaga gcccgaagat ggcctacaag
     2281 gcgcgcttcc gcccgctcga ggcgctgatc gacggcgact ggcagccctt tccgggcgat
     2341 ttcgcctgat cacggcgccc ggcggtcggg tcgactcgct acaatctcgc gatgctctac
     2401 gaactcgccc gccccctcct gttcgcgctc gaccccgaga ccgcccacaa cctcaccctg
     2461 catggcctgc actatgccgg caagctgctg cccgccgccg agccggagcc ggcgagcgcg
     2521 gtcgaggtca tgggcctgcg ctttcccaac cgcgtcggcc tcgccgccgg gctcgacaag
     2581 aacggcgagg cgatcgacgg cctcgcccgc ctcggtttcg gcttcctcga gatcggcacg
     2641 atcacgccgc gcccgcagcc gggcaacccc cgcccgcgca tgttccgcct gcccgaggtg
     2701 cggggcatca tcaatcgcat gggcttcaac aaccacggcg tcgacgtcct gctcgcgcac
     2761 gtgcgtgcgg cgaagtaccg cggcatcctc ggcatcaaca tcggcaagaa cttcgacacc
     2821 ccgatcgagc gtgccgccga cgactacctc gcctgcctgg aaaaagtgta cgccctggcg
     2881 agctacgtca cggtgaacat ctcctcgccc aacaccaaga acctgcgcca gctgcagggc
     2941 gaatccgaac tcgacgacct cctcggccgc ctcaaggcgg cgcagacccg cctcaccgag
     3001 caacatgggc gctacgtccc gctcaccctc aagatcgccc ccgacctcga cgacgcgcag
     3061 gtgagcaaca tcgccgacgc gctgcgccgc caccgcatcg acggcgtgat cgccaccaat
     3121 accacgatcg cgcgcgacaa ggtgcagggc atcgcccacg gcaacgaaca gggcggcctg
     3181 tcgggcgcgc cggtgttcga ggcttccacc gccgtggtgc gcaagctcgc gcacgcgctc
     3241 gccggcgagt tgccgatcat cgccgccgga ggcgtgctcg agggcgcaca ggcgcgcgcc
     3301 aagctcgagg ccggcgccgc tttggttcag ctctacagcg gactcatcta ccgcggcccg
     3361 gcactggtgc gcgaatgcgt gcgcgccacc gcggggtgag tccttccccg acctcttcct
     3421 tcacccggcg agcagcacgc cgcgctcgtc cgcagcggcg cccgtgccga ccttccgacc
     3481 ctccgaggag cccaagatga ctcatcgccg ccagttcgtc ctctccgccg ccgcgcttgc
     3541 ggtcgcagcc accctgccct tcgccgccca cgccgcggac gccgcgaagg tcggcttcgt
     3601 ctatgtgagc ccgatcggcg atgccggctg gacctaccag cacgacctcg gtcgcaagga
     3661 gctcgagaag acgctcggcg gcaggatcga gacgcgctac gtcgagaacg tcgcggaagg
     3721 tgccgatgcc gagcgcgtga tccgcgagtt cgccgccagc ggcagcaaga tcgtgttcgc
     3781 caccagcttc ggctacatga actacgtcga gcgcgtcgcc cgccagttcc ccaacgtcac
     3841 cttcctgcac gccaccggct acaagtcggc gaagaacttc gcgccctaca acgcgcgctt
     3901 ctacgagggc cgctacctca acggcgtgat cgccggcaag atgaccaagt ccaacgtcct
     3961 cggctacgtc ggcgccttcc cgatccccga ggtgctgcag ggcatcaacg cgttcaccct
     4021 gggcgcgcgc agcgtgaacc cgaacgtcga ggtgcgggtg atctgggcca acagctggta
     4081 cgacccgggc aaggagcgcg aggcggcgat gaccctgatc tcgcagggcg ccgacatgct
     4141 cacccaccac accgactcca ccgccaccgt ccaggccgcg gaagaaaagg gcgtgtacgc
     4201 cttcggctac cactccgaca tgtcgaagta cgcgcccaag tcccagctca ccgccaccac
     4261 ccaccactgg ggcgagttct acgcccgcac cgtcgacgcg gtgctcgggg gcacgtggaa
     4321 gccggagggc gtgtggggtg gcatgaagga cggcatgatc cgcctcgccc cgctcaaccc
     4381 ggcgattccg gccgacgtgc aggccatggt caaggaactc cagggccgca tcaccgccgg
     4441 cagcctgcat cccttcaccg gcccggtgct cgaccaggcc gggaaggaac gcctcgccgc
     4501 cggtgcggtg atggacgatg ccaccctggg caagatggac tacttcgtcc agggcgtcgg
     4561 ttcgcgcctg cccaccgcca agtaagcctg cttccccacc acgtcggttc catccggcga
     4621 cgagaccgcc ctcgtgcgga gcgggcagcc ccccgctcgc ttcacggcgt gggcgggtcg
     4681 gcgcgatccg ggcggtccgc ccacgcctcg tcgtcggccg gaaggccgtt gcgcaggcag
     4741 gccgagatcc cggtggcgat cgcgtcggcc atgcgcgcct ggcgttcggg gtcgcgcagc
     4801 aacagttcct cgtcgcggtt tttcaggatg ccggcttcga acagcactgc cggcatgcgc
     4861 gcgtggcgca gcaccgccag cccgtcgtag tggtgcacgg cgtgctcgcg gtcggcgtag
     4921 ctgcgccggc ggccgttctt gtgcgtgggc tcgaaaccca tgcgctgcag gcgggcgccg
     4981 atcgcacgcg cacacaggat gctcatggcc gtatcggggt tgtcgcgcga gacgaagagc
     5041 gaatgcccgg cgaactcgtc gttgtagtcg agcgcacgcc cctcccaggt ccacgggcgc
     5101 aactcgacgg cattgaccga gtcgtgatgc agcgagagca ggaaggccgc tccggcctcg
     5161 ccggcggcct gcggccgcgc gcgcaggctc gcgatgcggc cgtcggcgtt gatggtgctc
     5221 accgcccagc cgcgcgcgtg cagtgcatcg accacatgca cggaaagatc gcggttgaac
     5281 tcgaactcgc tgcggccgcg cgcgctcgtc gccccgggcg cgtccagggt gtggccgacg
     5341 tcgacagcca ccggcccggg cccctccccg ccagccagcg ccccccccgc gatcgccgcc
     5401 agcacgagcg caagcagccc gtggcgcggc gcacgccgcc acgcatcccc ggtccgtgcg
     5461 tgccccgagg cctgcaaaga cccgcaaagc gagcgcccat gcggatggaa aagttccact
     5521 cgggctacat cttgattcaa atcatggaga gcgcgggtgc ccgccgtgac aatccgccca
     5581 ccatcggcat tgccgtggac agttgtaccc ctgacccacg cgggcgaacc gagaccttcc
     5641 tgacaaaacg agacaagcga tgaggagtac tccatgagcg gtattttcac aaaatcgatg
     5701 gcgcggaaca tcttctacgg ggggacgacg ttcttcttcc tgttgttcct ggcgctcacc
     5761 ttcgacaccg agcgcgagct gcccaaccgg gacaaccgcg ccgccatcac ccccgaggtt
     5821 gccgccggca agcacatctg ggaaacccgc aactgtctgg gctgccacac gctgctcggc
     5881 gagggcgcct acttcgctcc cgaactcggc aacgtctaca agcgccgcgg cggcgacttc
     5941 atcaagggct ggatgcaggc catgcccacc ggagcccccg gccgtcgcca gatgccgcag
     6001 ttcaacctca ccgatgaaga gctcgaccag ctggtggcct tcctgaagta cacctcggaa
     6061 atcaacaccg ccaactggcc gcccaacatc gagggctgat cggaactctg gagaccacaa
     6121 ggggatatcc atgcaataca aatcacaagc ggtcgccatg ccctacttca tcgcggcgat
     6181 cggtctgttc gtggggcaaa tcatcttcgg cctgattctg ggcctgcagt acgtcgtcgg
     6241 ggacttcctg ttcccggaga tcccgttcaa cgtcgcgcgg atggttcaca ccaacctgct
     6301 gatcgtgtgg ctgctgttcg gcttcatggg tgcggcctac tacctgatcc cggaagagac
     6361 cgagaccgaa ctcttcagcc cgaagctggc gctggcgatg ttctggatct tcctggtggc
     6421 cggtgcgctg accatcgtgg gctacctgct cgtgccttac agcacgctgg cgtccatgac
     6481 gggcaacgac atcctcgcca ccatgggccg ggagttcctc gagcagccgc tgttgaccaa
     6541 gatcggcatc gtgatcgtcg cgctcgcctt cctgttcaac atcacgatga ccgtgctcaa
     6601 gggccgcaag accgtcatca gcatcgtgct gctgctcggc ttgtggggtc tggcgatctt
     6661 cttcctgttc tccttctaca acccggtcaa cctcgtccgc gacaagttct actggtggtg
     6721 ggtggttcac ctctgggtgg aaggcgtgtg ggaactgatc atggccgcga tgctcgcctt
     6781 cgtgctcatc aaggtcaccg gtgtagaccg tgaagtgatc gagaagtggc tgtacgtcat
     6841 catcaccctg gcgctcgtga ccggcatcat cggtaccggc caccactact tctggatcgg
     6901 cacgccggaa tactggcagt ggtggggttc gatcttctcc gcgctggagc cgatcccctt
     6961 cttcgccatg accgtcttcg cgttcaacat ggtcaaccgt cgtcgtcgcg agcacccgaa
     7021 caaggctgcc gtgctgtggg cgctcggtac cggcgtgatg gccttcctcg gcgctggcgt
     7081 gtggggcttc ctgcacaccc tggctccggt gaactact
//
LOCUS       DAOQBS010000141         7114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_10311_length_7114_cov_15.343816, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000141 DAOQBS010000000
VERSION     DAOQBS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10311_length_7114_cov_15.343816"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKN25_13075"
     CDS             <1..368
                     /locus_tag="PKN25_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNV91879.1"
                     /translation="FDVMVTGNMFGDILSDEASMLTGSIGMLPSASLNADNKGMYEPS
                     HGSAPDIAGKDLANPLATILSAAMMLRYTFNQEAAAQRIERAVKKVLAQGYRTGDIYE
                     PGTKRVGTREMGDAVLAAL"
     gene            423..1556
                     /gene="asd"
                     /locus_tag="PKN25_13080"
     CDS             423..1556
                     /gene="asd"
                     /locus_tag="PKN25_13080"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNV91880.1"
                     /translation="MNRVGLVGWRGMVGSVLMQRMVEEGDFAFIEPVYFSTSNAGGKA
                     PSFGGKEAAAPLQDASNIDALKSCDIVITCQGGDYTKEVFPKLRATGWNGHWIDAASA
                     LRMNDDAVIILDPVNRNVIDAALAKGGRNWIGGNCTVSLMLMGLGGLFRHGLVEWISA
                     MTYQAASGAGAQNMRELISQMGTIHDSVKDLLADPASAILEIDRKVAETMRSDAFPKK
                     NFRNTPLAGSLIPWIDVPVEHGQSKEEWKGGAECNKILGNPAFRTPGSIPIDGLCVRI
                     GAMRCHSQALTIKLKKDVPLDEITEIIAGANPWVKVVPNDREITERELTPTAVTGTLT
                     VPVGRLHKMAMGPEYLGAFTVGDQLLWGAAEPLRRMLRILLER"
     gene            1763..>2930
                     /locus_tag="PKN25_13085"
     CDS             1763..>2930
                     /locus_tag="PKN25_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNV91881.1"
                     /translation="MTKSVRASLLALSIAALPLSVQAAGLGQINVLSGLGQPLRAEIQ
                     ISASAQELQSLSARIASADAFRRANLSYGSAVSTIRVSVDTRAARPVVRLTSDRPIND
                     PFVDLLVELDWAGGNLVREYTFLLDPVELSAPRPLAAAIESPAARPVRGAPRAAVAAP
                     APMRAAPVAPAPADTWTVRRGDTMHRIASANAHPGTTLDQMLIALLRANPQAFDGGNI
                     NRLRAGTVLNVPGADAVRAIDAGEARREIRAQAADFDAYRRGLATSVAARAPEPAAVQ
                     ESGGRIVPRVQEPAAATEAGDKVTVSRSDTGADAGGEARLQVLEEELTTRSKALEEAN
                     ERLVRLERSIRDLQRLLEVQSGTMGQLQASSGAPAQSVAAAEAPVVTPPVEAAKA"
     assembly_gap    2931..3030
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3031..4845
                     /locus_tag="PKN25_13090"
     CDS             <3031..4845
                     /locus_tag="PKN25_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNV91882.1"
                     /translation="APAPAPKPPAAPAAAVEPAAAPGFVDTLMEDPTLLYGGGGILAL
                     LLAYAGLKARQRRKEEEIRAAEAALGAASVGSSGQSVFAGTGGQSVDTGATSIMHTDF
                     SHSGLSSIDTDEGVDPVAEADVYMAYGRDAQAEEILQDALKADPGRAAIHLKLLEIYA
                     QRRSTRQFETVASELFARSGGQGRDWEKAAALGRRLDPENPLYAAERGDTGAQRAPQG
                     EPAVAPTVAAAAGVAVAGAVATAAEAAPTGPELASLDFTAAAAAQPSHSQMRDTWTLP
                     GELNKFASGEREGPAGEPAGEGAPAAPLADEIPFDTGALDFELDLADDTPTAMPDLSA
                     TTPGLGREFDLTLEAADEEPPPPAPAFIPVSEPATDGGLTFDLDLGEPDEPFEAAARE
                     PQPASPAAIEAAPAPEPFASAPTISVEDSGTISNLDFELPGMEAPGGEAFDLDATVVS
                     ATGTLDDELAPPPAAPAGTADEAVVDLEKTAFDSSLLDFDFELDAPATPTQAAVPAGL
                     DLTSIDLDLEALPAEAGAAFDAVPFSVADTQFDQPAAGGAEAPGSMNEEAETKLELAR
                     AYEEMGDKEGALELLEEVVAEGTPRQQTTARDMIARLG"
     gene            4905..5522
                     /locus_tag="PKN25_13095"
     CDS             4905..5522
                     /locus_tag="PKN25_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HNV91883.1"
                     /translation="MHSGLVLLLWLAAVAAVQLLPSAALGVAVAACALAAAVFARART
                     WRLVRRIRVLLIAIAILFAWFTPGEAVLLDWPRLGPTREGLALAALHAGRLLAVVCAV
                     GILLERLPLARLVGGLYALSRPLRLVGVAPERLALRLLLVLRYVEASPRGQGPADWRH
                     WLADESPSDAAAEAAPVVLARERLGLGEGLLAGALFGALLWWSLA"
     gene            5519..6316
                     /gene="truA"
                     /locus_tag="PKN25_13100"
     CDS             5519..6316
                     /gene="truA"
                     /locus_tag="PKN25_13100"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNV91884.1"
                     /translation="MRRIVLGLEYAGDAFCGWQSQAHGRTVQDVLERALAQIAGEPVR
                     THCAGRTDTGVHASAQVAHFDTRAVRPASAWVRGVNALLPPAVVVRWSTEVDDDFHAR
                     FLACERQYRYILHNAPTRPAMLAGRVGWYHPALDVERMAAAAQCLVGTHDFSSFRAAG
                     CQAKTPVRVMHEARVVRQGEYLLFDFRANGFLHHMIRNLVGALVYVGKGRYPVAWMRE
                     LLEARDRARAAPTFSPDGLYLCGVAYPPRWSLPGDGRIIALPQLPLV"
     gene            6317..6940
                     /locus_tag="PKN25_13105"
     CDS             6317..6940
                     /locus_tag="PKN25_13105"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNV91885.1"
                     /translation="MFRTRIKICGLTRSQDVRAAVDSGADAIGFVFYPPSPRAVSIEQ
                     AAELVALLPPFVVSVGLFVNPAPGEVEAVLARVPLQLLQFHGDESESDCARHGRAWIK
                     AARMRPGVDLLEFAASYPRASGILVDAFVDGYGGGGRTFDWTLIPAGFKRPLVLSGGL
                     DADNVGDAVRRIRPWAVDVSSGVESAKGIKDADMIAAFIAGVRHADG"
     gene            6927..>7114
                     /locus_tag="PKN25_13110"
     CDS             6927..>7114
                     /locus_tag="PKN25_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNV91886.1"
                     /translation="MQMADTPYQFPDASGHFGPYGGIFVAETLMPALAELREAYAACQ
                     NDPAFVAEFEYELKHYVGR"
BASE COUNT         1020 a         2529 c         2420 g         1045 t
ORIGIN      
        1 agttcgacgt gatggtcacc ggcaacatgt tcggcgacat cctgtccgac gaggcctcga
       61 tgctcaccgg ctcgatcggc atgctgcctt cggcctcgct caacgccgac aacaagggca
      121 tgtacgagcc ctcgcacggc tccgcgcccg acatcgccgg caaggatctc gccaacccgc
      181 tcgccaccat cctgtcggcg gcgatgatgc tgcgctatac cttcaatcag gaggccgctg
      241 cccagcgcat cgagcgcgcg gtcaagaagg tgctggccca gggctatcgt accggcgaca
      301 tctacgagcc gggcacgaag cgcgtcggca cccgcgagat gggcgacgcc gtgctcgcag
      361 cgctgtaagc tcgcgcgctg gcaaggcttt ttcgaagaac gtttttgttg agagtgatga
      421 acatgaatcg agtcggtctg gttggctggc gtggcatggt cggttccgtg ctgatgcagc
      481 ggatggtgga ggagggcgac tttgccttca tcgagccggt ttatttttcc acctcgaacg
      541 ccggcggcaa ggcgccgtcc ttcggcggca aggaagcggc cgcgccgctg caggacgcca
      601 gcaacatcga cgcgctgaag tcctgcgata tcgtgatcac ctgccaaggc ggcgactaca
      661 ccaaggaagt cttccccaag ctgcgcgcca ccggctggaa cggccactgg atcgacgccg
      721 cctcggcgct gcgcatgaac gacgacgcgg tgatcatcct cgacccggtc aaccgcaacg
      781 tcatcgatgc cgcgctcgcc aagggcgggc gcaactggat cggcggcaac tgcaccgtgt
      841 cgctgatgct gatgggcctg ggcggcctgt tcaggcacgg cctggtggag tggatctcgg
      901 cgatgaccta ccaggcggcc tcgggcgccg gcgcgcagaa catgcgcgaa ctcatcagcc
      961 agatgggcac catccacgac tcggtcaagg acctgctcgc cgacccggcc tcggccatcc
     1021 tcgagatcga ccgcaaggtt gccgaaacca tgcgctcgga cgccttcccg aagaagaact
     1081 tccgcaacac cccgctcgcc ggcagcctca tcccgtggat cgacgtcccg gtcgagcacg
     1141 gccagagcaa ggaagaatgg aagggcggcg ccgagtgcaa caagatcctc ggcaacccgg
     1201 ccttccgcac cccgggctcg atcccgatcg acggcctgtg cgtgcgcatc ggggcgatgc
     1261 gctgccactc gcaggcactc accatcaagc tcaagaagga cgtaccgctc gacgagatca
     1321 ccgagatcat cgccggtgcc aacccgtggg tgaaggtggt gcccaacgac cgcgagatca
     1381 ccgagcgcga gctcacgccg accgccgtca ccggcacgct caccgtcccg gtcggccgcc
     1441 tgcacaagat ggcgatgggt cccgagtacc tcggcgcctt caccgtcggc gaccagctgc
     1501 tgtggggggc ggccgagccg ctgcgccgca tgctgcgcat cctgctcgag cgctgagcgt
     1561 ttcgcggcag tctggcaagg ggggcgaaag cccccctttt tgttgcgaac tcgtgtcgaa
     1621 aggcggtatt tcttcacacc ggacgggtac gaatcaggcc cggctaatgc gatgcaagcc
     1681 gggaaatgct agattcctag gccagacgcg gtgaaatcga ccgtgatgct ccgttgcggg
     1741 ggaacgagtt ttgagatcca aaatgacaaa atcggtccgg gcttccctgc tcgccctctc
     1801 catcgccgcg cttccgctgt ccgtgcaggc ggccgggctc ggtcagatca acgtgctcag
     1861 cggattgggt cagccgctgc gcgccgagat ccagatctcc gccagcgcgc aggaactgca
     1921 gtcgctgagc gcccgcatcg cctcggcaga cgcgttccgg cgtgcgaacc tgagctacgg
     1981 ctccgcggtg agcacgatcc gcgtcagcgt ggatacgcgc gccgcgcgtc ccgtcgtccg
     2041 tctcaccagc gaccggccga tcaacgatcc cttcgtcgac ctgctcgtcg agctcgactg
     2101 ggcgggcggc aatctggtgc gcgaatacac cttcctgctc gatccggtcg agctctccgc
     2161 cccgcgaccg cttgcggcgg cgatcgagag tccagcggcg cggcccgtgc gcggcgcgcc
     2221 acgcgccgcc gtggcggcgc ccgcgccgat gcgtgccgcg cccgtcgcgc ccgcgcccgc
     2281 cgacacctgg acggtgcggc gcggcgacac catgcaccgc atcgcgagcg cgaatgcgca
     2341 ccccggcacc acgctcgacc agatgctgat cgcgctgctg cgcgcgaacc cccaggcctt
     2401 cgacggcggc aatatcaacc gtttgcgcgc cggaacggtc ctcaatgtcc ccggcgccga
     2461 tgccgtgcgc gccatcgacg ccggcgaggc gcgccgcgag atccgcgccc aggccgccga
     2521 cttcgacgcc taccggcgcg gcctcgcgac ttcggtcgcg gcacgcgcgc ccgaacccgc
     2581 cgctgtacag gagagcggcg gacgtatcgt gccgcgcgtc caggagcccg ccgcggcgac
     2641 cgaggcgggc gacaaggtga cggtctcgcg cagcgacacc ggtgcggacg cgggcggaga
     2701 ggcgcgcctg caggtgttgg aagaagaact caccacgcgt tcgaaggcgc tcgaggaggc
     2761 caacgagcgt cttgtccggc tcgagcgcag cattcgcgac ctgcagcgcc tgctcgaggt
     2821 gcagagcggc accatgggcc agttgcaggc ctcgtccggc gcgcccgccc agagcgtggc
     2881 ggcggccgaa gcacccgtcg tgaccccgcc agtcgaggcg gccaaggcac nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gctccggcgc ccgcgcccaa gccgcccgcc
     3061 gcgccggccg cggcagtcga gcccgcagcc gcacccggct tcgtcgatac cctgatggag
     3121 gatccgaccc tgctctacgg cggtggtggc atcctcgcgc tgttgctcgc ctacgcaggc
     3181 ctgaaggcgc gccagcgccg caaggaagaa gagatccgtg ctgccgaggc cgccctcggt
     3241 gccgcctcgg tgggcagcag cggccagagc gtgttcgcgg gcaccggcgg ccagagcgtc
     3301 gataccggtg ccaccagcat catgcacacc gacttcagcc attccgggct gtcctcgatc
     3361 gataccgacg agggcgtgga cccggtggcc gaggcggacg tctacatggc ctacggtcgt
     3421 gacgcgcagg cagaggaaat cctgcaggat gccctcaagg ccgatccggg ccgggccgcg
     3481 atccatctca agctgctcga gatctatgcc cagcgccgca gcacgcgcca gttcgagacc
     3541 gtggcgagcg agcttttcgc acgctccggc ggccaggggc gcgactggga gaaggctgct
     3601 gcgctggggc gccggctcga tccggagaat ccgctctacg ctgcggagcg tggcgacacg
     3661 ggtgcgcagc gcgcgccgca gggcgagccc gcggtggcgc cgacggtcgc tgcggcagcg
     3721 ggcgtcgctg tcgcaggcgc cgtcgcgacc gcagccgagg ccgcaccgac cgggccggag
     3781 ctggcctcgc tcgatttcac cgcggcggcc gctgcgcagc ccagccactc gcagatgcgc
     3841 gacacgtgga cattgccggg agagctgaac aagttcgcct cgggcgagcg tgagggtccg
     3901 gcaggcgagc cagccggaga gggggcgccg gctgcgcccc tggccgacga gatccccttc
     3961 gacaccggtg cgctcgactt cgagctcgac ctcgcggatg acacgcccac cgcgatgccc
     4021 gacctgtcgg cgaccacgcc cggcctcggt cgcgagttcg atctcaccct ggaagccgcg
     4081 gacgaggagc cgcctccccc ggccccagct ttcattcccg tttccgagcc ggccactgat
     4141 ggcggtctga ccttcgacct cgatctcggc gagcccgacg agcccttcga ggccgcggcg
     4201 cgcgagccgc agccggccag ccccgccgcg atcgaagccg cacccgcacc cgagccgttc
     4261 gcctccgcgc cgacgatctc ggtggaagac tccggcacga tcagcaacct cgacttcgag
     4321 ctccccggca tggaggctcc gggcggcgag gccttcgatc tcgatgcgac cgtggtcagc
     4381 gccaccggca cgctcgacga cgagctcgcg ccgccgccgg cggcccctgc gggcacggcc
     4441 gacgaggccg tggtcgacct cgagaagacc gccttcgact ccagcctgct cgacttcgac
     4501 ttcgagctcg acgcaccggc gaccccgacc caggccgcgg tgccggccgg gctcgacctg
     4561 acctcgatcg atctcgacct cgaggcgctg cccgccgaag ccggtgccgc gttcgatgcg
     4621 gtgccgttct cggtggccga cacccagttc gaccagccgg ccgcaggcgg cgcggaagcg
     4681 ccggggtcga tgaacgagga ggccgagacc aagctcgaac tggcccgcgc ctacgaggag
     4741 atgggcgaca aggaaggtgc gctcgaattg ctcgaggaag tggtcgccga ggggacgccg
     4801 cgacagcaga ccacggcccg cgatatgatc gcgcgcctgg gctgaacgcc cccgccggca
     4861 ggccgccacg agcgcgggct cgcggcggcc ttttccgttt accgatgcac tccggccttg
     4921 tcctgctcct ctggctcgcg gcggtcgccg ccgtccagct cctgccgtcc gctgcgctcg
     4981 gcgtcgcggt cgcggcctgt gcgctcgcgg ccgccgtctt cgcgcgcgcg cgcacctggc
     5041 gcctcgtgcg gcggatccgc gtgctgctga tcgcgatcgc catcctcttc gcctggttca
     5101 ccccgggcga ggcggtgctg ctcgactggc cgcgcctggg gccgacacgc gaggggcttg
     5161 cgctcgcggc gctgcatgcc gggcggctgc tcgccgtggt gtgcgcggtc ggcatcctgc
     5221 tcgagcgcct gccgctcgcg cgcctggtcg gtggcctgta cgcgctgtcg cgcccgctgc
     5281 gcctggtcgg cgtggcgccc gaacgcctcg ccctgcgcct cttgctggtg ctgcgctacg
     5341 tcgaggcctc gccgcgcggg caggggccgg ccgactggcg acactggctg gcagacgagt
     5401 ctccgtccga cgcggccgcc gaagcggcgc cggtggtgct ggcgcgcgag cgcctcggac
     5461 tgggcgaggg gctgctggcg ggtgcgctct tcggcgcgct gctgtggtgg agcctggcat
     5521 gaggcgcatc gtgctcggcc tcgaatacgc cggcgacgcc ttctgcggct ggcagagcca
     5581 ggcccatggc cgcaccgtgc aggacgtgct cgagcgcgcg ctcgcgcaga tcgcgggcga
     5641 gccggtgcgc acccactgcg ccggacgcac cgacaccggc gtgcatgcga gcgcgcaggt
     5701 ggcgcatttc gacacccgcg ccgtgcgccc ggcctcggcc tgggtgcgcg gtgtcaacgc
     5761 gctgctgccg ccggccgtgg tggtgcgctg gtcgaccgag gtggacgacg acttccacgc
     5821 ccgcttcctg gcctgcgagc ggcagtaccg ctacatcctg cacaatgcgc cgacgcggcc
     5881 ggcgatgctg gccgggcggg tgggctggta ccacccggcg ctcgacgtgg agcgcatggc
     5941 cgccgccgcg cagtgcctgg tcggcaccca cgacttctcc agttttcgcg ctgccggctg
     6001 tcaggcgaag acgccggtgc gggtgatgca cgaggcccgc gtggtccgcc agggcgagta
     6061 cctgctgttc gatttccgcg ccaacggctt cctccaccac atgatccgca acctggtcgg
     6121 cgcgctggtg tacgtcggca aggggcgtta tccggtggcg tggatgcgcg agctgctcga
     6181 ggcccgcgac cgcgcgcgcg cggcgccgac cttctccccg gacgggctct acctgtgcgg
     6241 cgtggcctac ccgccgcgct ggtcgctgcc gggtgatggg cgtatcatcg cgctgcccca
     6301 gctacccctc gtctgaatgt tccgtacccg aatcaagatc tgcggcctga cccggtcgca
     6361 ggacgtgcgc gcggctgtcg atagcggtgc cgacgcgatc ggcttcgtgt tctacccgcc
     6421 cagcccgcgt gcggtctcca tagagcaggc tgccgagctc gtcgcgctgc tgcccccctt
     6481 cgtcgtctcg gtcggcctct tcgtcaatcc cgctcccggc gaggtcgagg cggtactcgc
     6541 gcgcgttccg ctgcaacttc tgcagttcca cggtgacgaa agcgagtccg actgtgcccg
     6601 tcacgggcgc gcgtggatca aggcggcgcg aatgcggccc ggggtcgatc tgctagaatt
     6661 cgcggcttcg tacccgcgcg cgagcggcat cctggtcgac gccttcgtcg acggctatgg
     6721 cggcgggggc aggaccttcg actggacgct gatccccgca ggcttcaagc gtccgctcgt
     6781 gctttcgggc ggactcgacg cggacaacgt gggcgacgcc gtgcgccgca tccgcccgtg
     6841 ggcggtggat gtgtcgagcg gggtcgagag tgccaaggga atcaaggacg cggacatgat
     6901 cgccgcgttc atcgccggag ttcgacatgc agatggctga cacgccttac cagtttcccg
     6961 acgcgagcgg gcatttcggg ccctacggcg gcatcttcgt cgccgaaacc ctgatgccgg
     7021 cgctcgccga gctgcgcgag gcctacgcgg cctgccagaa cgatccggcg ttcgtcgccg
     7081 agttcgagta cgagctcaag cactacgtcg gccg
//
LOCUS       DAOQBS010000142         7086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_10379_length_7086_cov_18.187740, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000142 DAOQBS010000000
VERSION     DAOQBS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10379_length_7086_cov_18.187740"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /gene="efp"
                     /locus_tag="PKN25_13115"
     CDS             complement(<1..535)
                     /gene="efp"
                     /locus_tag="PKN25_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNV91887.1"
                     /translation="MKTAQELRSGNVIMVGSDPLVVQKAEYNKSGRNSAVVKMKFKNL
                     LTGAPSESVYKADDKFEVVVLDHKEVTYSYFADPMYVFMDADYEQYEVEADNMTDALK
                     YLEDGLQCEVVFYNGKAISVELPNSVVREVVYTEPAVKGDTSGKVMKPAKISTGFELP
                     VPAFVEIGDKIEIDTRTD"
     gene            complement(601..1788)
                     /gene="earP"
                     /locus_tag="PKN25_13120"
     CDS             complement(601..1788)
                     /gene="earP"
                     /locus_tag="PKN25_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HNV91888.1"
                     /translation="MHRFRSPARPRWEIFCQVVDNFGDIGVCWRLATDLARRGGGAVR
                     LWVDEWEALRRICPAATAVDERAGGSVQGVELRRWTSAFVPLVPGEIVVEAFACELPA
                     AQLEAMAALRQAPLWINLEYLSAEAWVAGCHGMASPHPRLPLVKHFFFPGFDAGTGGL
                     PREADLLARRDAWRARPEGRAAWLAARGIASGPDALRVSLFAYEQPELPALFDAWAGS
                     GREMLVLVPESRVLGDVQRALGRERLQAGDRVVRGALTACVLPFTDQAGYDELLWACE
                     LNFVRGEDSFVRAQWAARPFVWQIYPQADAAHHDKLEAFLARYLQGLPAAEADALARF
                     WRAWNGCAPDRATPAEAWPALAAALPALDVHARRWCDVQAALPDLATALDTFCSHIAH
                     AAR"
     gene            2156..3118
                     /locus_tag="PKN25_13125"
     CDS             2156..3118
                     /locus_tag="PKN25_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91889.1"
                     /translation="MHGRKLLPLALILAFPALAIAQSAQQQGLPSPEAAQEQALLEQS
                     RTQVRGFSVWLGETVNDWFGDRPFEEGGKVTNGRLGLRNLWRQDEGWNTNVRFRARFD
                     LPNLRDKAYVFIGRQNERELVTDQSEAFTREQLLLEERRKEDQTGFAGVGVALRKNLD
                     FRVGVRGGMKFYTQARYRKQWALTELDRIEFRETVFWNNKDKFGSTTALDYEHAFTPS
                     LSLNWQNATTISEKTDDFAWGSSLGLFKRFGDDRILSGEMLVTGETGAEENVDEYGVR
                     MKWTQPVYRDWLIGEFIVGHYWPQREDRERERAWGVGVNLEMRF"
     gene            complement(3382..4128)
                     /locus_tag="PKN25_13130"
     CDS             complement(3382..4128)
                     /locus_tag="PKN25_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HNV91890.1"
                     /translation="MRPMQLERLLHAQGFGSRKDCRALIRAGYVSVAGEVCDDPTEEF
                     DPGSRDGSPGLEFTVDDQPWRFRAQAYVVLHKPAGYECSQKPTFHPSVFTLLPPQLLA
                     RGVQCVGRLDQDTTGLLLLSDDGQFIHQWSSGKKRTPKVYEVTLKHAVEQDFADRLVA
                     GVELNDEPVPIAAAACELLSPTTLRLTICEGKYHQVKRMVGAAGNRVEVLHRSRVGGL
                     DLPNELAPGQWRWLETEDLARLADYADATS"
     gene            4255..4722
                     /locus_tag="PKN25_13135"
     CDS             4255..4722
                     /locus_tag="PKN25_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNV91891.1"
                     /translation="MKFEHLIEVNDLANPLLTPLTREELWFGLLCRAEDARAFLPGLE
                     ACRIVERGEGSLVRDLHFGHAVIRDRVNFDTLRWIRFETEANAQHAGGSLTITIEEPE
                     PGALFLRFAYHTSLPDQGEDARYAEFVRSAYHQSDLDTVRIIRMIAASGGLEQ"
     gene            4915..5925
                     /locus_tag="PKN25_13140"
     CDS             4915..5925
                     /locus_tag="PKN25_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HNV91892.1"
                     /translation="MWFKNLQVYRLPANWDMSAERLEEQLAKKPFHPCGSQDMESRGW
                     LSPLGNEVLVHAVGGQWLVCLGFEHRLLPSAVVKQEADERAEELAEQQGYKLGRKQLK
                     ELREQVTQELMPRAFTRRRRMFAWIDPINGWLVVDAASQSKAEDMLEQLRHTLDSFPL
                     TLLRTERSPMSAMADWLAGGEAPDGFTIDQDCELRSVAEDKAAVRYARHPLEGDDVKG
                     HLEAGKLPTRLALTFDDRISFVLTEKLEIKRLDFLDIVRDQVGEADKDDAEALFNAEF
                     ALMTGELAHLLPAVVAALGGELAGAAVETGVSAELPRVQKEMAAARPVSAQTVAADDP
                     PF"
     gene            6418..6873
                     /locus_tag="PKN25_13145"
     CDS             6418..6873
                     /locus_tag="PKN25_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNV91893.1"
                     /translation="MNDTMFLEWWHWEIAGIALVLLELALPSFFIIWFGLGAMLTGFV
                     LLAMPDLALSQQIAVWIIASMAMTVLWFSFAKRARVRTLIGTAAGEVIGEVGLLVSAV
                     APFERGKVRFQRPVLGADEWACVAESAIAAGERVKVVSVEGSYVKVTKA"
     gene            6894..>7086
                     /locus_tag="PKN25_13150"
     CDS             6894..>7086
                     /locus_tag="PKN25_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91894.1"
                     /translation="MIMSEGLAIAIAVLVFVVITIAKGVRLVAQGEEWVVERLGKYHA
                     TLRPGLNILIPYLDRVAYKL"
BASE COUNT         1254 a         2344 c         2347 g         1141 t
ORIGIN      
        1 cgtcggtgcg ggtgtcgatc tcgatcttgt cgccgatctc gacgaaggcc ggcacgggca
       61 gctcgaagcc ggtgctgatc ttggccggct tcatgacctt gcccgaggtg tcgcccttga
      121 cggcgggctc ggtgtagacg acctcgcgca ccacgctgtt gggcagctcg accgagatcg
      181 ccttgccgtt gtagaacacc acctcgcact gcaggccgtc ctcgaggtac ttgagcgcgt
      241 cggtcatgtt gtccgcttcg acttcgtact gctcgtagtc ggcatccatg aacacgtaca
      301 tcgggtccgc gaagtaggag taggtgactt ccttgtggtc gaggacgacg acctcgaact
      361 tgtcgtcggc cttgtagacc gactccgacg gcgcaccggt gagcaggttc ttgaacttca
      421 tcttcacgac cgcggagttg cggccggact tgttgtattc ggccttctgc accaccaacg
      481 ggtcgctgcc gaccatgatg acgttgcccg agcggagttc ctgagcggtt ttcatgctgt
      541 ttcctgaatg gttcgacgcg actcgtggtc gcatgtcctt gaaaaaatta gcgcgcgatt
      601 ctaccgcgcg gcgtgcgcga tgtgggaaca gaatgtatcg agtgcggtcg cgagatcggg
      661 cagcgccgcc tgcacatcgc accagcggcg agcatggaca tcgagcgcgg gcagcgccgc
      721 ggcgagggcc ggccaggcct cggccggcgt cgcacgatcc ggcgcgcagc cgttccaggc
      781 gcgccagaag cgggcgagcg catccgcctc ggccgccggc agcccctgca ggtagcgcgc
      841 caggaaagcc tcgagcttgt cgtgatgggc ggcatccgcc tgcggataga tctgccacac
      901 gaagggtcgc gccgcccatt gcgcgcgcac gaaggagtcc tcgccgcgca cgaaattcag
      961 ctcgcaggcc cacagcagct cgtcgtagcc cgcctggtcg gtgaagggca gcacgcaggc
     1021 ggtgagcgcg ccgcgcacca cccgatcgcc cgcctgcagg cgctcgcgac cgagcgcacg
     1081 ttgcacgtcg ccgagcacgc gcgactcggg caccagcacc agcatctcgc gaccgctgcc
     1141 ggcccaggcg tcgaacaggg cggggagctc cggctgctcg taggcgaaca gcgacacccg
     1201 cagcgcatcc gggccgctcg cgatgccgcg cgcggccagc caggcggcgc gtccctcggg
     1261 acgggctcgc caggcgtcgc ggcgcgcgag caggtcggcc tcgcgcggca atccgccagt
     1321 gcccgcatcg aagcccggaa aaaagaaatg cttcaccagc ggcaggcgcg gatgcggcga
     1381 ggccatgccg tgacagcccg ccacccaggc ctcggccgac aggtactcga ggttgatcca
     1441 caacggcgcc tgccgcagcg ccgccatggc ctcgagctgg gcggccggca gctcgcaggc
     1501 gaaggcctcg acgacgatct cgcccggcac gagcggaacg aacgcgctcg tccagcgccg
     1561 caactccacc ccttgcacgc ttccgcccgc ccgctcatcc accgcggtgg cggccggaca
     1621 gatgcgccgc aaggcctccc attcatccac ccagagccgc accgctccac ctccacggcg
     1681 tgccagatcg gtcgccagcc gccagcacac gccgatatcc ccaaaattat ccacaacctg
     1741 acagaagatc tcccagcggg gacgggcggg cgaccggaat cgatgcatgg tggggacaat
     1801 cctggataaa caatggggac agcttgtgaa caagccgact tcacgcgatc ggccggaaaa
     1861 ggctcaacaa aacgcgcaca gccgaccaag gggcttctca accgcctttt gaagaacacg
     1921 aacgctttgc cggcaaggtc tccgggtact tatccacaag aaacgcgcca ctgttaactt
     1981 ctttgttatt taaatcaata gaaaaacaga agaacaaacc cgcagccgcc acggccgggc
     2041 caccaagctt gcccagcgca cgagagtgcc ggcatgatgc cggccatggg tggtcgcttc
     2101 cccgaccatg aaccacccga gaggccgcca ggcccatgaa tcaggatcct tccccatgca
     2161 tggccgcaaa cttcttccgc tcgccctgat cctcgccttt cccgcgctcg caatcgccca
     2221 atcggcgcaa cagcaaggac ttccctcccc cgaggcggcg caggaacagg ctctgctgga
     2281 gcagagccgc acgcaggtgc gcggtttctc ggtatggctg ggggaaacgg tgaatgactg
     2341 gttcggcgat cgtcccttcg aggagggcgg caaggtgacc aatgggcgtc tggggctacg
     2401 caacctgtgg cgtcaggacg aaggctggaa taccaacgtg cgtttccgtg cgcgtttcga
     2461 tctccccaac ctgcgcgaca aggcctacgt tttcatcggc cggcagaacg agcgcgaact
     2521 cgtgaccgac caatccgaag cctttacgcg cgaacagctc ctgctcgaag aacgccgcaa
     2581 ggaagaccag accggttttg ccggcgtggg ggtggccttg cgcaagaacc tcgatttccg
     2641 tgtcggtgtg cgcggcggaa tgaagttcta cacccaggcg cgttatcgca agcagtgggc
     2701 gctgaccgaa ctggaccgga tcgaattccg cgaaacggtg ttctggaaca acaaggacaa
     2761 gttcggctcg accaccgcgc tggactacga gcacgccttc acgccctcgc tctcgctgaa
     2821 ttggcagaat gcaacgacga tttcggaaaa gaccgacgat ttcgcctggg gtagcagcct
     2881 ggggctgttc aagcgcttcg gtgacgatcg gatcctgtcg ggcgagatgc tggtgaccgg
     2941 agaaaccggt gccgaagaaa acgtggatga atacggcgtg cggatgaaat ggacccagcc
     3001 ggtctatcgc gactggttga tcggcgagtt catcgtcggc cactactggc cgcagcgcga
     3061 ggaccgcgag cgcgagcgcg cctggggggt cggggtgaac ctcgagatgc gcttctgagc
     3121 ttccgaggcc tcgtaggagc gacgcaagtc gcgatcggcg cgccggttcg tgcggcactc
     3181 gtctcgccac cgcaatcgcg acttgcgtcg ctcccacggg agacagcgcg gcgtatcggc
     3241 gccgtaggag cgacgcaagt cgcgatcggg cctcgatagc accgtccgct cgagggcgag
     3301 cgtccaatcg cgacttgcgt cgctcctacg gaaaccggtg cggaggccgg cgcatgcggc
     3361 ggagcgcgat ggcaggcagg ctcaggaagt cgcgtcggcg tagtcggcca ggcgggccag
     3421 gtcctcggtc tcgagccagc gccactgccc gggcgccagc tcgttcggca ggtcgagccc
     3481 gcccacgcgg ctgcggtgca gcacctcgac acggttgccg gcggcgccga ccatgcgctt
     3541 gacctgatga tacttgccct cgcagatcgt cagccgcagc gtggtcggac tgagcagctc
     3601 gcacgccgca gccgcgatcg gaacgggctc gtcgttcagt tcgacgccgg cgaccagccg
     3661 gtcggcgaag tcctgttcca ccgcatgctt gagcgtcacc tcatagacct tgggcgtacg
     3721 cttctttccc gagctccact ggtggatgaa ctggccgtcg tcggagagca gcagcaggcc
     3781 ggtcgtgtcc tggtcgagcc ggcccacgca ctgcacgccg cgggcgagca gctgcggtgg
     3841 cagcagggtg aacaccgagg gatggaaggt cggcttctgc gagcattcgt agcctgcagg
     3901 cttgtgcagg accacgtagg cctgggcgcg gaagcgccag ggttgatcat cgacggtgaa
     3961 ttcgagcccg ggcgaaccat cgcggctgcc ggggtcgaat tcctcggtgg ggtcgtcgca
     4021 gacttcgccg gcgacgctga cgtagccggc acggatgagg gcacggcaat ccttgcggct
     4081 gccgaagccc tgggcgtgga ggaggcgttc gagttgcatg ggacgcatgt tagcagggcg
     4141 agcgccgcgg caagatcgat gccccggctc accgtgcagg atgggggcga ccgaggtcgt
     4201 gccgtgcgcg ggccgagcgg gtaaactgcc gccctgcccg ctcgggagaa gcacttgaaa
     4261 ttcgaacacc tgatcgaggt caatgatctc gccaacccgc tgctgacccc gctcacccgc
     4321 gaagagctgt ggttcggcct gctctgccgc gccgaggacg cgcgcgcctt cctgcccggc
     4381 ctggaggcct gccgcatcgt cgagcgcggc gagggcagcc tggtccgcga tctgcacttc
     4441 ggccacgccg tgatccgcga ccgggtgaac ttcgacacct tgcgctggat ccgcttcgaa
     4501 accgaggcca acgcacagca tgccggcggc agcctcacga tcaccatcga ggaacccgag
     4561 cccggcgccc tcttcctgcg tttcgcctac cacacctcgc tgcccgacca gggcgaggac
     4621 gcgcgctacg cggaattcgt gcgctcggcc taccaccagt ccgacctcga caccgtgcgc
     4681 atcatccgca tgatcgccgc gtccggcggg ctcgaacagt agggccccca aggactcccg
     4741 acggcgctgc ggcggccggc ttgccatggc agcgtgctgc cgtgcgcgag tggcctggcc
     4801 agcgaacttc ccgtgcggtg gcgttgcatg tgcgggccgg acggctcgca cagccgtttt
     4861 tcgctcccga gcgtcgtctc atcttccctc tcaccaacag gaaacccctt cgtcatgtgg
     4921 ttcaagaatc tgcaggtcta tcgccttccc gccaactggg acatgagcgc cgagcgcctc
     4981 gaggaacagc tcgcgaagaa gcccttccac ccctgcggca gccaggacat ggagagtcgc
     5041 ggctggctgt cgccgctggg caacgaggtg ctggtgcacg ccgtgggcgg ccagtggctg
     5101 gtctgcctcg gcttcgagca ccgcctgctg ccctccgcgg tggtcaagca ggaggccgac
     5161 gagcgcgccg aggagctggc cgagcaacag ggctacaagc tcggtcgcaa gcagttgaag
     5221 gagctgcgcg agcaggtgac gcaggaactg atgccgcgcg ccttcacccg ccgccgccgc
     5281 atgttcgcct ggatcgaccc gatcaatggc tggctggtgg tggatgccgc cagccagagc
     5341 aaggccgagg acatgctcga gcagctgcgc cacaccctcg acagcttccc gctgaccttg
     5401 ctgcgcaccg agcgctcgcc gatgtcggcg atggccgact ggctggccgg tggcgaggcc
     5461 ccggacggct tcaccatcga ccaggactgc gagctgcgct cggtggccga ggacaaggcc
     5521 gcggtgcgct atgcgcgtca cccgctcgaa ggcgacgacg tcaagggcca cctggaagcc
     5581 ggcaagctgc cgacccggct ggcgctgacc ttcgacgacc gcatcagctt cgtgctgacc
     5641 gagaagctcg agatcaagcg tctcgatttc ctcgacatcg tgcgcgatca ggtgggcgaa
     5701 gccgacaagg acgacgccga ggcgctgttc aacgccgaat tcgcgctcat gaccggcgag
     5761 ctcgcccacc tgctgccggc ggtcgtggcc gcgctcggcg gcgagcttgc aggcgccgcg
     5821 gtggaaaccg gcgtgagcgc cgagctgccg cgtgtgcaga aggaaatggc cgccgcgcgg
     5881 ccggtgtccg cgcagaccgt cgccgcggac gatccgccgt tctgagcctc gcagcgcctg
     5941 gcaggtttgg cacgggcgca cgggccgggc tgctgcatcg cgacttgcgt cgctcccacg
     6001 gtacggctcc agaagagcgg tggattctgt aggagcgacg caagtcgcga tacaggccgt
     6061 cgagatggaa atgctgcacc gatggggggt tcgagccgca agcaggggtc cgcttcttgg
     6121 tgtacacaga atttaaagat ttaaaagatc ataaagatca tgtaccgccc gattctgtgg
     6181 acaacccggt ttttcatggc tgaatcagcg tcttgcatca agcaaaaccc ggtgcacgat
     6241 ggccgcggga aacgagtacg gtttgtggat gaaaaacccg ccgtcgccgc cttcacgact
     6301 tatccacaat ttccgatccc cttcggtcac agctttccca ctgcccgaaa aacgccacac
     6361 gggaaccgat cctcacccgc gaacccgttc cccggcgagc tgcgctaaag tctgcccatg
     6421 aacgacacga tgttcctcga atggtggcac tgggagatcg ccggcatcgc gctggtgctg
     6481 ctcgagctcg ccctgccctc cttcttcatc atctggttcg ggctcggtgc gatgctcacc
     6541 ggattcgtgc tgctcgcgat gccggacctg gcgctgtcgc agcagatcgc ggtctggatc
     6601 atcgcctcga tggcgatgac cgtgctgtgg ttcagcttcg cgaagcgcgc gcgcgtcagg
     6661 accctgatcg gcaccgccgc cggcgaggtg atcggggaag tgggcctgct ggtgagcgcg
     6721 gtcgcgccct tcgagcgcgg caaggtgcgc ttccagcgcc cggtgctcgg cgccgacgaa
     6781 tgggcctgtg tggccgagag cgcgatcgcg gccggcgagc gcgtgaaggt ggtgagcgtg
     6841 gagggcagct acgtcaaggt gacgaaggcc tgagcaacaa ggaaagcaag gaaatgatca
     6901 tgtcggaagg actcgcgatc gcgatcgcgg tgctggtgtt cgtggtgatc acgatcgcca
     6961 agggggtgcg tctggtggcg cagggcgagg aatgggtggt cgagcgcctg ggcaagtacc
     7021 acgccacgct gcgccccggg ctcaacatcc tcatccccta cctcgaccgc gtcgcctaca
     7081 agctgg
//
LOCUS       DAOQBS010000143         7084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_10383_length_7084_cov_13.718168, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000143 DAOQBS010000000
VERSION     DAOQBS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10383_length_7084_cov_13.718168"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="PKN25_13155"
     CDS             complement(<1..1298)
                     /locus_tag="PKN25_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV91895.1"
                     /translation="MSASSVILGFQPVLLWTDALLFLLVALALLAGMRARAQAPLRAA
                     WRKVGRRPTGMAAATVLLAFLVVGLVDSLHYRPLLPALERVAGADGGGPASGAAPAAP
                     VVSTEVLSALDALLAPLRLRTEKTYSAPFAWTLYQRETMETADGGQVRDYPRLAHGAA
                     HLGADPGPARAGDLALRLAKALAQAALAWLLAFVVLASLVWRGSGLRWGEVAAALLAG
                     HTVLAWRAALVTLAVLLAVVGVCVELAPLYHVLGTDKVGQDVLYLALKSVRTALVIGT
                     LTTLVMMPFAVALGIAAGYLGGWVDDAVQYVYTVLNAIPGVLLIAAAVLMMQVVIDTN
                     PHWFDTAAERSDARLLALCFILGITSWTGLARLLRGETLKLRELEYVQAARAFGVRTP
                     AILRRHILPNLMHIVLIALVMDFSGLVLAEAVLSYVGIGV"
     gene            complement(1295..2272)
                     /locus_tag="PKN25_13160"
     CDS             complement(1295..2272)
                     /locus_tag="PKN25_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV91896.1"
                     /translation="MPAYLLRRLLYAVPILIGVNLLTFVLFFVVNSPDDMARMHIGVK
                     RATPEAIERWKVERGYDKPMFINDAASGSARFTETLFFDKSLRMFALEFGRADDGRDI
                     GQEIRERMGPSLAIALPTFVLSLVVSISFALLLVFFRATWLDFWGVVLCVAMMSISSL
                     FYIIGGQWLVSKLWALVPISGYAPGLDAARFLVLPVLISVVAGIGSSARWYRTIFLEE
                     ISKDYVRTARAKGLSELAVLFRHVLRNALIPILTGVVVVIPLLFMGSLLVESFFAIPG
                     LGSYTIDAINAQDFAVVRAMVFIGSVLYIVGLILTDLSYTLADPRVRLQ"
     gene            complement(2272..4491)
                     /locus_tag="PKN25_13165"
     CDS             complement(2272..4491)
                     /locus_tag="PKN25_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV91897.1"
                     /translation="MLIACLALAACGPVWNDPYPVAERGEHILYTAFTERPKHLDPVQ
                     SYSEDEASFLYQIVEPPLQYHYLKRPYVLEPATAEAMPSLRRLDAGGRELPANADPAK
                     VVRTVVEVRIRPGILYQPHPAFALDEDGGPRYLGLGEADLRAVRGLGDFAATGTRELE
                     ARDYVYQIKRLAHPRLHSPIFELMAEYLPGLQELQAGLVAATKEGGASGAAAKDDPPG
                     WLDLDRFALPGVEVVDRHTLRITLQGAYPQFLYWLSMPFFSPVPREVDRFFAQPGMAE
                     RNLTLDWWPVGTGPYMLVENNPNARMVLARNPNYRGDAYPCAGESGDAEAGLLGDCGK
                     PMPFIDKVVFSREREGIPYWNKFLQGYYDASGVSSDNFDQAVSLTSQGEVTLSDDMRD
                     KGIRLLTSVSPSIFYLGFNMLDPLVGGGASKAEKERARKLRQAISIALDMEEFVSIFL
                     NGRGLPGMSPLPPGIFGARAGRAGMNPVVYEWQGSEADGHPVRRGVDEARRLLAEAGW
                     PNGRDAQTGEPLVIHLDTTPGGLGDKARSDWLAKKFRALGVQFVVRPTDFNRFQDKIR
                     QGNAQLFFFGWNADYPDPENLLFLLHGPQGKVKFNGQNAANYENPEYDALFERMKAMP
                     DGPARQQIIDRMVAMLQHDAPWIFAFHPMSYSLQHGWVLNRKTGAMVRNTMKYQRIDL
                     ERRAAARTEWNAPVTWPLLLVAALLAALVAPAAIHWRRRETATAAPGAAGSGARGGR"
     gene            4828..5625
                     /gene="fabI"
                     /locus_tag="PKN25_13170"
     CDS             4828..5625
                     /gene="fabI"
                     /locus_tag="PKN25_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HNV91898.1"
                     /translation="MGFLAGKRILITGLLSNRSIAYGIAKAMHREGAELAFTYQNDRF
                     KDRVAKLAADFGSSLIFPLDVQDDAQITGLFEQLGQHWDGLDALVHSIAFAPGEALEG
                     DFLDGLTREAFRISQEVSTYSFPALVKAARPMMQGRNASVLTLSYLGAVRTMPNYNIM
                     GLAKASLEASVRYLAVTLGPEGIRANAISAGPIKTLAASGIGSFGKLLAFNEHNAPLR
                     RNVTIEEVGNAAAFMCSDLASGITGEVMYVDGGFNTTALGNALPLPQ"
     gene            complement(5783..>7084)
                     /locus_tag="PKN25_13175"
     CDS             complement(5783..>7084)
                     /locus_tag="PKN25_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNV91899.1"
                     /translation="LAADVKVDDAQVSAYYEANPTRFERPARLQAEYLVFDRAAVERG
                     IEVADAAVRAFYDGNPQRFGVPEERQARHILLALDQGAEQAEVDRVMAEARALVEQLR
                     ADPARFAALAKEKSQDPGSAGNGGDLGFFARGVMVGAFEDAVFGLQKGVIGEPVRSDF
                     GVHIVEVTDIKPSSVKPFEAVRAEILAELRAQEAGRRFAELAEQFSNTVYEQPDSLQP
                     AAEALGLKTATTDWISRDSAPAPLADERVISALFGDEAVQKSRNTEAIEIGNGTLVSA
                     RVKAFEPARRLPLDEVRERIVELVRIEQGRVKATRAGEEMLAALRRGEQAAARWGEAR
                     KLQRGAPGLPAAAMQAVFSASAETLPVHVGVQSTEGDYVLYRIEAVERAQIGDADPRV
                     AAVTGQYKQLLAGRDFGAFLSELRQRYEVTINPTALQVEPR"
BASE COUNT         1139 a         2534 c         2408 g         1003 t
ORIGIN      
        1 tcgacgccga tgccgacgta ggacagcacc gcctcggcca gcaccagccc ggagaagtcc
       61 atcaccagcg cgatcagcac gatatgcatc aggttgggca ggatgtggcg gcgcaggatc
      121 gccggcgtgc gcaccccgaa ggcgcgcgcc gcctgcacgt actcgagctc gcgcagcttg
      181 agcgtctcgc cgcgcaacag gcgcgccagc cccgtccagc tggtgatgcc gaggatgaag
      241 cacagtgcca gcaggcgcgc atccgagcgc tcggctgcgg tgtcgaacca gtgcggattc
      301 gtgtcgatga cgacctgcat catcagcacc gcggcggcga tcaacagcac tccggggatc
      361 gcgttgagca cggtgtatac gtactgcacg gcatcgtcca cccagccgcc aaggtaaccc
      421 gcggcgatgc cgagcgcgac cgcgaagggc atcatcacca gcgtcgtcag ggtgccgatc
      481 accagcgccg tgcgcacgct cttgagcgcc aggtagagca cgtcctgccc gaccttgtcg
      541 gtgccgagca cgtggtagag cggcgccagc tccacgcaca cccccaccac cgcgagcagc
      601 accgccagcg tcaccagcgc cgcgcgccag gccagcacgg tgtgtccggc cagcaaggcc
      661 gcggccacct cgccccagcg cagcccgctg ccgcgccata ccaggctggc gagcacgacg
      721 aaggcgagca gccacgcgag cgccgcctgc gcgagcgcct tcgccagccg cagcgcgagg
      781 tcgcccgccc gcgccgggcc cgggtcggcg ccgaggtggg ccgcgccgtg ggcgagacgc
      841 ggatagtcgc gcacctggcc gccgtcggcg gtctccatcg tctcgcgctg gtacagcgtc
      901 cacgcgaagg gcgcggaata ggtcttctcg gtgcgcaggc gcagcggcgc gagcagggcg
      961 tcgagcgccg acagcacctc ggtggaaacg accggagccg ccggtgctgc tccgcttgcc
     1021 ggcccgcccc catccgcccc cgccacgcgc tccagcgccg gcagcagcgg ccggtagtgc
     1081 aggctgtcga ccaggcccac gacgaggaag gccagcaaca cggtggccgc cgccatcccg
     1141 gtcggtcgcc tccccacctt gcgccaggcc gcgcgcagcg gcgcctgcgc acgcgcgcgc
     1201 atgcccgcca gcagcgccag cgcgacgagc aggaagagca acgcgtcggt ccacagcagc
     1261 accggctgga agcccaagat cacgctcgat gcgctcattg cagcctcacc cgcggatcgg
     1321 ccagggtgta ggacaggtcg gtgaggatca agccgacgat gtacagcacc gagccgatga
     1381 acaccatcgc ccgcaccacc gcgaagtcct gcgcgttgat cgcgtcgatc gtgtagctgc
     1441 ccaggccggg aatcgcgaaa aaggactcca ccagcaggct gcccatgaag agcagcggga
     1501 tcaccaccac caccccggtc aggatcggga tcagcgcatt gcgcagcaca tggcggaaca
     1561 gcaccgcgag ctccgacagc cccttcgcgc gcgcggtgcg cacgtagtcc ttcgagatct
     1621 cctcgaggaa gatcgtgcgg taccaccgcg ccgaagagcc gatgccggcc accacgctga
     1681 tcagcaccgg caggaccagg aagcgcgctg catccagccc gggcgcgtag cccgagatcg
     1741 gcaccaatgc ccacagcttg gacaccagcc actggccgcc gatgatgtag aacaggctgg
     1801 agatcgacat catcgccacg cacagcacca cgccccagaa atccagccag gtggcgcgga
     1861 agaagaccag cagcagcgcg aagctgatcg acaccacgag gctgagcacg aaggtcggca
     1921 gcgcgatcgc cagcgagggc cccatgcgct cgcggatctc ctggccgatg tcgcggccgt
     1981 cgtcggcgcg gccgaactcg agcgcgaaca tgcgcagcga cttgtcgaag aagagggtct
     2041 cggtgaagcg cgcgctgccg ctcgcggcgt cgttgatgaa catcggcttg tcgtagccgc
     2101 gctcgacctt ccagcgctcg atggcctcgg gcgtggcgcg cttgacgccg atgtgcatgc
     2161 gcgccatgtc gtcgggcgag ttcaccacga agaagagcac gaaggtgagc aggttcaccc
     2221 cgatcaggat cggcaccgcg tagagcaggc ggcgcagcag gtaggccggc atcagcgccc
     2281 tcctcgcgcg ccggacccgg ccgcgcccgg cgccgcggtc gcggtctcgc gccggcgcca
     2341 gtggatcgcc gccggcgcca ccagcgcggc gagcagcgcc gccaccagca gcagcggcca
     2401 ggtcaccggc gcgttccact ccgtgcgcgc agcggcacgg cgctcgaggt cgatgcgctg
     2461 gtacttcatg gtgttgcgca ccatggcccc ggtcttgcgg ttgagcaccc agccatgctg
     2521 cagcgaatac gacatcgggt ggaaggcgaa gatccacggc gcgtcgtgct gcagcatcgc
     2581 caccatgcgg tcgatgatct gctggcgcgc ggggccgtcg ggcatggcct tcatgcgctc
     2641 gaacagcgcg tcgtattccg ggttctcgta gttggccgcg ttctggccgt tgaacttcac
     2701 cttgccctgc gggccgtgga gcaggaagag caggttctcc gggtcggggt agtctgcgtt
     2761 ccagccgaag aagaagagct gcgcgttacc ctggcggatc ttgtcctgga agcggttgaa
     2821 gtcggtcggc cgcacgacga actgcacgcc cagcgcgcgg aacttcttcg ccagccagtc
     2881 cgagcgcgcc ttgtcgccca gcccgccggg cgtggtgtcg aggtggatca ccagcggctc
     2941 gccggtctgc gcatcgcgtc catttggcca gccggcctcg gcgagcaggc ggcgcgcctc
     3001 gtcgacgcca cgacgcacgg gatggccgtc cgcctcgctg ccctgccact cgtacaccac
     3061 cgggttcatc cccgcccgtc ccgcgcgcgc gccgaagatg cccggcggca aggggctcat
     3121 gccgggcagg ccgcggccgt tgaggaagat cgacacgaac tcctccatgt cgagcgcgat
     3181 cgagatcgcc tggcgcagct tgcgcgcgcg ctccttctcc gccttcgagg cgccgccgcc
     3241 cacgagcggg tcgagcatgt tgaagccgag gtagaagatc gacggcgaca ccgaggtgag
     3301 caggcggatg cccttgtcgc gcatgtcgtc cgagagcgtc acctcgccct ggctggtgag
     3361 actgacggcc tggtcgaagt tgtccgaaga cacgcccgag gcgtcgtaat agccttgcag
     3421 gaacttgttc cagtatggga tgccctcgcg ctcgcgcgag aacaccacct tgtcgatgaa
     3481 tggcatgggc ttgccgcagt cgccgagcaa cccggcctcg gcatcgccgg actcgcccgc
     3541 gcagggatag gcgtcgccgc ggtagttcgg attgcgcgca agcaccatgc gcgcgttggg
     3601 gttgttctcg accagcatgt acggcccggt gcccaccggc caccagtcca gcgtcaggtt
     3661 gcgctcggcc atgccgggct gggcgaagaa gcggtccacc tcgcgcggca ccgggctgaa
     3721 gaagggcatc gaaagccaat acaggaactg cggataggcg ccctgcaggg tgatgcgcaa
     3781 ggtgtggcgg tccaccacct ccacgccggg cagcgcgaag cggtcgagat ccagccagcc
     3841 gggcgggtcg tccttcgccg cggcaccgct cgccccgcct tccttggttg ccgcaaccag
     3901 cccggcctgc agctcctgga gcccgggcag atactccgcc atgagctcga agatcggcga
     3961 gtgcaggcgc ggatgggcga gacgcttgat ctgatagacg tagtcgcgcg cctcgagctc
     4021 gcgcgtgccg gtcgcggcga agtcgccgag accccgcact gcgcgcaggt cggcctcgcc
     4081 aaggccgaga tagcgcgggc cgccatcctc atcgagggcg aaggccgggt ggggctggta
     4141 caggatgccc gggcggatac gcacctcgac cacggtacgc accaccttgg ccgggtcggc
     4201 gttcgcgggc agctcgcggc cgccagcgtc gagccggcgc aggctcggca tggcctcggc
     4261 ggtggcgggc tcgagcacat aggggcgctt caggtagtgg tactgcagcg gcggctcgac
     4321 gatctggtac aggaagctcg cctcgtcctc gctgtaggac tgcaccggat cgagatgctt
     4381 ggggcgctcg gtgaaggcgg tgtacaggat gtgttccccg cgctcggcca ccggataggg
     4441 gtcgttccac accgggccgc aggccgcgag tgccaggcag gcgatcaaca gcgcagccgc
     4501 ggaacgtaac gacgcacccg ccctgcgcgg gtgcgcaggg cggatcgggc cggggcggcc
     4561 gggggtccgg agagggcagg agaaccggcg cacgggcacg cggaagcttc gatgaaagcg
     4621 cggattctgg caggtttcgc acgcgcttgc gatctgcaca cggcctcgga cccacagccg
     4681 tgacccgccc gcctcgacga aacatggcct gccctaccgg ctccgtcgcc tccttccatc
     4741 cccccgcgcg ggcgaaagtc ccgtccgtcg aagggttttg acggtttttc gctataatcc
     4801 ccgtcttgtc attcggagtc atttctcatg ggctttctcg ccggcaaacg aatcctcatc
     4861 accggtctgc tcagcaaccg ttcgattgcc tacggcatcg ccaaggccat gcaccgtgaa
     4921 ggcgccgagc tcgccttcac ctaccagaac gaccgcttca aggaccgcgt cgccaagctg
     4981 gcggccgact tcggcagcag cctgatcttc ccgctcgacg ttcaggacga cgcgcagatc
     5041 accggcctgt tcgagcagct cgggcagcac tgggacggcc tcgacgcgct ggtgcattcg
     5101 atcgcgttcg cccccggcga ggcactcgag ggcgatttcc tcgacggcct cacccgcgag
     5161 gccttccgca tctcgcagga ggtcagcacc tacagcttcc cggcgctggt caaggccgcg
     5221 cgcccgatga tgcagggccg caacgccagc gtgctgaccc tgtcctacct gggtgccgtg
     5281 cgcaccatgc ccaactacaa catcatgggt ctcgccaagg ccagcctgga agcctcggtg
     5341 cgctacctcg ccgtcaccct cggcccggaa ggcatccgcg ccaacgcgat ctccgccggc
     5401 ccgatcaaga cgctcgcggc ctcgggcatc ggcagcttcg gcaagctgct cgccttcaac
     5461 gagcacaacg ccccgctgcg ccgcaacgtg accatcgagg aagtgggtaa cgccgccgcc
     5521 ttcatgtgct cggacctggc cagcggcatc accggcgagg tcatgtacgt ggatggcggc
     5581 ttcaacacca ccgcgctcgg caacgcgctg ccgctgccgc agtaaggatc gcggcggact
     5641 gcgctgcgcg gcagccgccg catccgcctg ccgagcctcg gcggacggca caaaaaagcc
     5701 cggtttcgac accgggcttt ttgtttccat gctgcccccg ccgcacgggc gagtggcgga
     5761 gcatgcgggg atccgctggg aatcagcgcg gctcgacctg cagtgccgtc gggttgatcg
     5821 tgacctcgta gcgctggcgc agctcgctca ggaaggcgcc gaagtcgcgc ccggccagga
     5881 gctgcttgta ctgtcccgta accgcggcga cgcgcgggtc cgcgtcgccg atctgcgcgc
     5941 gctcgaccgc ctcgatgcgg tacagcacgt agtcgccctc ggtcgactgc acgccgacgt
     6001 gcaccgggag cgtctcggcc gacgccgaga acaccgcctg catggccgcc gccggcagcc
     6061 cgggggcacc acgctgcagc ttgcgcgcct cgccccagcg cgctgcggcc tgctcgcccc
     6121 tgcgcagcgc ggccagcatc tcctcgcccg cacgggtggc cttcacccgg ccctgctcga
     6181 tgcggacgag ttcgacgatg cgctcgcgga cctcgtcgag cggcaggcgg cgcgccggct
     6241 cgaaggcctt gacccgcgcc gacaccaggg tgccgttgcc gatctcgatg gcctcggtgt
     6301 tgcggctttt ctgcaccgcc tcgtcgccga acagggccga gatcacgcgc tcgtcggcga
     6361 gcggcgccgg ggcggagtcg cggctgatcc agtcggtggt ggcggtcttg aggccgagcg
     6421 cctcggccgc gggctgaagg ctgtcgggct gttcgtacac cgtgttggag aactgctcgg
     6481 ccagctccgc gaaacgacga ccggcctcct gggcgcgcag ctcggcgagg atctccgcgc
     6541 gcacggcctc gaagggcttg accgaggaag gcttgatatc ggtgacctcg acgatgtgca
     6601 cgccgaagtc gctgcgcacc ggctcgccga tcacgccttt ctgcagaccg aacaccgcgt
     6661 cctcgaaggc gccgaccatc acgccgcggg cgaagaaacc gagatcgccg ccgttgccgg
     6721 ccgagcccgg atcctgcgac ttctccttcg cgagggcggc gaagcgcgcg ggatcggcgc
     6781 gcagttgctc gacgagcgct cgcgcctcgg ccatcacgcg atcgacctcg gcctgctcgg
     6841 cgccttggtc gagggcgagc aggatgtgac gcgcctggcg ctcctcgggc acgccgaagc
     6901 gctgcggatt gccgtcgtag aaggcgcgca ccgcggcgtc cgcgacctcg atgccgcgct
     6961 cgaccgccgc gcggtcgaag accaggtatt cggcctgcag ccgcgccggg cgctcgaagc
     7021 gcgtcgggtt ggcctcgtag taggcgctga cctgcgcgtc gtccaccttg acgtcggccg
     7081 ccag
//
LOCUS       DAOQBS010000144         7017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_10533_length_7017_cov_15.183568, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000144 DAOQBS010000000
VERSION     DAOQBS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10533_length_7017_cov_15.183568"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKN25_13180"
     CDS             <1..462
                     /locus_tag="PKN25_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNV91900.1"
                     /translation="SKIDSRTILDQMGAETLLGMGDMLYLAPGTGLPVRVHGAFVADD
                     EVHKVVDHLKKIGPPDYIEGILSSAEDDVDAALGGGGEGDDGESDALYDQAVEIVVKT
                     RRPSISLVQRHLRIGYNRAARLIEQMERAGLVSPMGSNGNREVIVPAKESE"
     gene            459..1118
                     /gene="lolA"
                     /locus_tag="PKN25_13185"
     CDS             459..1118
                     /gene="lolA"
                     /locus_tag="PKN25_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HNV91901.1"
                     /translation="MIQFNTRPAALLRGVAGVVLALAALGATAAGGVQQLRDFVTGAR
                     SAEGEFEQVVTAQSGRRPQEASGSFAYARPGRFHWEYDRPYRQVLVGDGKTLWSWDPD
                     LNQVTVRAIGEALGATPAAILFGSGALDDNFELADAGERDGLAWAEARPKRTESGFES
                     LRIGLAEGQLRRMEMRDHFGQTTVIRFTRLRANPVLAEDRFRFEPPAGADVIGELPGA
                     R"
     gene            1189..2613
                     /locus_tag="PKN25_13190"
     CDS             1189..2613
                     /locus_tag="PKN25_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNV91902.1"
                     /translation="MTDLFDALEPPQVPLAERMRPHTLDEVAGQSHLLGPGKPLRLAF
                     ESRRPHSMILWGPPGVGKTTLARLMARGFDAEFVALSAVFSGVKEIREAIQQAQAAKA
                     RGRHTILFVDEVHRFNKAQQDAFLPYVEQGLVTFIGATTENPSFEVNSALLSRAAVYV
                     LESLDHDAMLRLFERAHAIACPTLGFDEDARERLIGFADGDARRLMNLIEQVQVAAET
                     AKVDPVSATFVDQALSRNLRRFDKGGEAFYDQISALHKSVRGSDPDASLYWLCRMLDG
                     GADPLYLGRRLIRMAVEDIGLADPRALEIALNACHTYERLGSPEGELALAQATIFMAC
                     AAKSNAAYKAYKAARAFVAHDGSRPVPLHLRNAPTKLMKALDYGKAYRYAHDEPEAYA
                     AGENYFPEGMQSPGWYQPTPRGLEGKIGQKLAWLRELDRKAAEGGKQYARPSPSAGGA
                     SREVPASKGTPGEERISGGGDDPS"
     gene            complement(2585..2848)
                     /locus_tag="PKN25_13195"
     CDS             complement(2585..2848)
                     /locus_tag="PKN25_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91903.1"
                     /translation="MLSIRDCLDYCDLTDEEVLLIAEHEDIPDAAAAQLCCGLVQTPE
                     GVLALTHYMRELIERANACGDVRKAKLAASVCERFMKDHPLPH"
     gene            3090..4382
                     /gene="serS"
                     /locus_tag="PKN25_13200"
     CDS             3090..4382
                     /gene="serS"
                     /locus_tag="PKN25_13200"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNV91904.1"
                     /translation="MLDIQLLRTQLDHVAARLATRGLQLDTAAFQALEDERKQLQTRT
                     QELQARRNALSKQIGMLKGKGEDASAVMAEVAQLGDGLKACEQALPMLLERINAFLAT
                     LPNLPQDDVPAGADETGNVEVRRWGTPRAYDFEVRDHVDLGAPLGLDFETGAKLSGAR
                     FAFLRGPVARLHRALAQFMLDTHTREHGYTECYTPYIVNGSALYGTGQLPKFKEDLFW
                     VLRGGDEEGLEQYLIPTAEITLTNSVREEVLALESLPIRLTAHSPCFRSEAGSGGRDV
                     RGMIRQHQFDKVEMVQIVEPAKSKEALEQMVGHAEAILQKLELPYRVITLCTGDMGFS
                     AAKTYDLEVWLPAQNTYREISSCSNCESFQARRMQARFKNAQGKNELVHTLNGSGLAV
                     GRTLVAVLENHQQADGSIVIPRALVPYMGGIERIEAPL"
     gene            complement(4492..5490)
                     /locus_tag="PKN25_13205"
     CDS             complement(4492..5490)
                     /locus_tag="PKN25_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNV91905.1"
                     /translation="MSDTHALLGLPAGASAQQIKRAFRKLAMRWHPDRNPDPAAADQF
                     RRLRDAHDRLLAALLEGKEKAHEDAAATQPPPQRGADRWQTLEISIEESFTGARRTVE
                     VERRSPCVLCGGSGIEKLSVSRLCTPCRGSGRLHGPAGLERCPGCEGRGYRNTGPCGG
                     CGGSGEQMQTRHLEVLIPPGVVDDDELRLAGEGEAHPEPRHRAGDLHLQIRLTSHPLF
                     RREGRDLVLRRPVSALRLLIGGTLRVPHPTGVRSIELEPGLAHPRSLRVPGAGFPAHG
                     RRARGDLVVEFEPELPHAPERSLHGLIDELDRALAARAEHHFPELARWESVWLDEH"
     gene            5687..6526
                     /locus_tag="PKN25_13210"
     CDS             5687..6526
                     /locus_tag="PKN25_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV91906.1"
                     /translation="MSPSRASETQPRDSWVHHPQGRIFAREWTPSGAAGERPASPIVL
                     LHDSLGCVELWRDFPAELSAASGRRVIAYDRLGFGRSDAREALPALDFIAEEAQAYFP
                     AVREQLGLERFVVFGHSVGGGMAVNCAAAFAGACDALITESAQVFPEDLTLSSIAEAK
                     EQFVDESQLQRLARYHGDKARWVLDAWTESWLHPGFAAWSLRSVLPQVRCPVLAIHGM
                     HDEYGTSVHPETIGELSAGPAQVAILPDTFHVPHRERPAAVIELVCAFLEPVRQGGAV
                     CVS"
     gene            complement(6668..6744)
                     /locus_tag="PKN25_13215"
     tRNA            complement(6668..6744)
                     /locus_tag="PKN25_13215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6708..6710),aa:Arg,seq:acg)
     gene            complement(6787..6863)
                     /locus_tag="PKN25_13220"
     tRNA            complement(6787..6863)
                     /locus_tag="PKN25_13220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6827..6829),aa:Arg,seq:acg)
     gene            complement(6926..7015)
                     /locus_tag="PKN25_13225"
     tRNA            complement(6926..7015)
                     /locus_tag="PKN25_13225"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6979..6981),aa:Ser,seq:cga)
BASE COUNT         1113 a         2384 c         2417 g         1103 t
ORIGIN      
        1 agcaagatcg actcgcgcac catcctcgac cagatgggcg ccgagaccct gctcggcatg
       61 ggcgacatgc tctacctcgc gcccggtacc gggctgccgg tgcgcgtgca cggtgccttc
      121 gtcgccgacg acgaggtgca caaggtggtc gaccacctca agaagatcgg tccgcccgac
      181 tatatcgagg gcatcctttc ctcggccgag gacgatgtcg acgccgcgct cggcggcggc
      241 ggggaaggcg atgacggcga gtcggacgcg ctctacgacc aggccgtgga gatcgtggtg
      301 aagacgcgcc ggccctcgat ctcgctggtg cagcgccacc tgcgcattgg ctacaaccgc
      361 gccgcgcgcc tgatcgaaca gatggagcgc gccggcctgg tgtcgccgat gggcagcaat
      421 ggcaaccgcg aagtgatcgt gccggccaag gagagcgaat gatccagttc aacacacgac
      481 cggccgcgct gctgcgcggt gtggcgggcg tcgtgctcgc gctggcggcc ctgggcgcta
      541 cggcggcggg tggcgtgcag cagctgcgcg acttcgtcac tggcgcgcgc agcgcggagg
      601 gcgagttcga gcaggtcgtc accgcgcagt ccgggcgccg acctcaggag gcctcgggca
      661 gcttcgccta cgcccgcccg gggcgcttcc attgggagta cgaccggcct tatcgccagg
      721 tgctggtggg cgacggcaag accctgtggt cctgggatcc cgacctcaac caggtgaccg
      781 tacgcgcgat cggcgaggcg ctcggcgcca ccccggcggc gatcctgttc ggcagcggcg
      841 cgctcgacga caatttcgag ctcgccgacg ccggcgagcg ggacggtctg gcctgggccg
      901 aggcgcgtcc gaagcgcacc gagagcggct tcgagtcgct gcgcatcggg ctggctgaag
      961 gccagttgcg gcgtatggag atgcgcgacc acttcggcca gaccacggtg atccgcttca
     1021 cccgcctgcg cgccaacccg gtgctcgccg aggaccgctt ccgcttcgag ccgcccgcag
     1081 gcgccgacgt gatcggcgaa ctgcccggcg cgcgctgatt ccccacgcac gcgtgcgcac
     1141 gccgcaatcg atccccgagt ccatgggccg tctgcaaggg ggctccgcat gaccgacctg
     1201 ttcgacgccc tcgagccgcc gcaggtgccg ctcgctgagc gcatgcggcc gcacaccctc
     1261 gacgaggtcg ccggccagtc acacctgttg ggaccgggca agccgctgcg actggccttc
     1321 gagtcgcgcc ggccgcactc gatgatcctg tggggcccgc ccggcgtcgg caagaccacg
     1381 ctcgcgcggc tgatggcgcg cggcttcgac gccgagttcg tcgccctgtc cgcggtgttc
     1441 tccggggtca aggagatccg cgaggcgatc cagcaggccc aggccgccaa ggcgcgcggt
     1501 cgccacacca tcctcttcgt cgacgaggtg caccgcttca acaaggcgca gcaggacgct
     1561 ttcctgccct acgtcgagca gggcttggtg accttcatcg gcgccaccac cgagaaccct
     1621 tcgttcgagg tcaactccgc gctgctgtcg cgcgccgcgg tgtatgtgct ggagtcgctc
     1681 gaccacgatg ccatgctgcg cctgttcgag cgcgcgcacg cgatcgcctg cccgacgctc
     1741 ggcttcgacg aggatgcgcg cgagcgcctg atcggctttg ccgacggcga cgcgcgcagg
     1801 ctgatgaacc tgatcgaaca ggtccaggtt gccgccgaga ccgcgaaggt cgatccggtg
     1861 agcgccacct tcgtcgacca ggccctgtcg cgcaacctgc gccgcttcga caagggcgga
     1921 gaggccttct acgaccagat ttccgccctg cacaagtcgg tgcgcggctc ggatccggat
     1981 gcctcgctgt actggctgtg ccgcatgctc gacggcggcg ccgatccgct ctacctcggc
     2041 cggcggctga tccgcatggc ggtggaggac atcggcctgg ccgatccgcg tgcgctcgag
     2101 atcgcgctca atgcctgcca cacctacgag cgcctcggtt ctcccgaggg cgaactcgcg
     2161 ctcgcccagg cgacgatctt catggcctgc gcggccaagt ccaacgccgc ctacaaggcc
     2221 tacaaggccg cgcgcgcctt tgtcgcccac gacggctcgc gtccggtgcc gctgcacctg
     2281 cgcaacgcgc cgaccaagct gatgaaggcg ctcgactacg gcaaggccta ccgctacgcg
     2341 cacgacgagc ccgaggccta tgcagcgggc gagaattatt tccccgaggg aatgcagtcg
     2401 cccgggtggt accaacccac tccgcgcggc ctcgaaggca agatcggcca gaagctcgct
     2461 tggctgcgcg aactcgaccg caaggcggcg gagggcggaa aacaatacgc gaggccgtcg
     2521 ccatctgcgg gcggcgcctc gcgcgaagtc ccggcgagca aaggtacgcc gggcgaggag
     2581 agaatcagtg ggggagggga tgatccttca tgaaacgctc gcagacggaa gccgccagtt
     2641 tggccttgcg cacgtccccg catgcattgg cacgctcgat gagttcgcgc atgtagtgcg
     2701 tgagtgccag cacgccctcc ggcgtctgca cgagcccgca gcaaagctgt gcggcggcgg
     2761 catccgggat gtcctcatgc tcggcaatca acaacacctc ctcgtcggtg agatcgcagt
     2821 agtccaggca gtcgcgaatg gatagcatgc cgtttcctcc aggtccgggc cgtctgatgc
     2881 ggctttgtcg tcaactcaag gatagtggac cgatccgctt ttacaagttc gatagaaatt
     2941 cagaaccaga accaagtcgt tgattaagaa agccgatttt gcgaagcgcg aggtcttgtc
     3001 gcgcaacccc gccatgtaat cagtcgcctg cgacggtttt ccgagaatcc gcatcaactt
     3061 ttgccgatcg cttttcagga acgtttccga tgctcgatat ccagcttctc cgcacccagc
     3121 tcgaccacgt cgccgcacgc ctcgccacgc gcgggctgca gctcgacacc gccgccttcc
     3181 aggccctcga ggacgagcgc aagcagttgc agacccgcac ccaggaactg caggcccgcc
     3241 gcaacgcgct gtccaagcag atcggcatgc tcaagggcaa gggcgaggac gcctccgcgg
     3301 tgatggccga ggtcgcccag ctcggcgacg ggctcaaggc ctgcgagcag gccctgccga
     3361 tgctgctcga gcgcatcaac gccttcctgg ccaccctgcc gaacctgccg caggatgatg
     3421 ttccggcggg cgcagacgag accggcaacg tcgaggtgcg gcgctggggt acgccgcgcg
     3481 cctacgactt cgaggtgcgc gaccacgtgg acctcggcgc tccgctcggc ctcgacttcg
     3541 agaccggcgc caagctgtcg ggcgcgcgct tcgccttcct gcgcggtccg gtggcgcgcc
     3601 tgcatcgcgc gctcgcgcag ttcatgctcg acacccacac ccgcgagcac ggctacaccg
     3661 agtgctacac gccctacatc gtcaatggct cggcgctcta cggcaccggt cagctgccca
     3721 agttcaagga ggatctgttc tgggtgctgc gcggcggcga cgaggagggc ctcgagcagt
     3781 acctgatccc gaccgcagag atcacgctca ccaacagcgt gcgcgaggag gtgctcgcgc
     3841 tcgagagcct gccgatccgt cttaccgcgc atagcccctg cttccgctcc gaggcgggca
     3901 gcggcgggcg cgacgtgcgc ggcatgatcc gccagcacca gttcgacaag gtcgagatgg
     3961 tgcagatcgt cgagccggcg aagagcaagg aggcgctcga acagatggtc ggccacgccg
     4021 aggccatcct gcagaagctc gagctgccct accgggtgat cacgctgtgc accggcgaca
     4081 tgggcttctc ggctgcgaag acctacgatc tcgaggtgtg gctgccggcg cagaacacat
     4141 accgcgagat ctccagctgc tcgaactgcg agtccttcca ggcgcgccgc atgcaggccc
     4201 gcttcaagaa cgcgcagggc aagaacgagc tggtgcacac gctcaacggg tccggtctgg
     4261 cggtggggcg caccctggtt gcggtgctgg agaaccacca gcaggccgac ggttcgatcg
     4321 tgatcccgcg ggcgctggtg ccctacatgg gtggcatcga gcgcatcgaa gcgccgctct
     4381 gagcggcgat cggcggttgg cgcgggtccg accttgcggc ccgcgccagc cgcgtcctgg
     4441 cggggccgtg ccgtgtgcga tgccgtcccg ctccgccgct tgcgcccgcg ctcagtgctc
     4501 gtccagccac accgactccc agcgcgcgag ctcggggaag tggtgctcgg cgcgcgcggc
     4561 gagcgcgcgg tcgagttcgt cgatcaggcc gtgcagcgag cgttcgggcg cgtgggggag
     4621 ttcgggctcg aactccacga cgaggtcgcc acgtgcgcgc cggccgtgcg ccggaaagcc
     4681 ggcgccgggt acgcgcaggc tgcgcggatg ggcgaggccg ggctcgagtt cgatgctgcg
     4741 cacgcccgtc gggtggggca cgcgcagggt cccgccgatc agcaggcgca gcgcgctgac
     4801 cgggcggcgc agcaccaggt cgcggccctc gcggcgaaac agcggatgac tggtgaggcg
     4861 gatctgcagg tgcaggtcgc cagcgcgatg gcgcggctcg gggtgcgctt cgccctcgcc
     4921 ggcgagacgc agttcgtcgt cgtccaccac cccgggtgga atgagtacct cgagatgacg
     4981 agtctgcatt tgttccccgc tgccgccaca gccgccacac ggtcccgtgt tgcgatagcc
     5041 ccgcccctcg cagcccgggc agcgctccag ccccgcaggt ccgtgcaagc ggcccgagcc
     5101 tcggcagggt gtgcataggc ggctcacgct gagcttctcg atgccgctgc cgccgcacag
     5161 cacgcagggg ctgcggcgct cgacctcgac cgtccgccgc gccccggtga agctctcctc
     5221 gatgctgatc tccagtgtct gccagcggtc cgcgccgcgc tgcggcggag gctgggtggc
     5281 agccgcgtcc tcgtgtgcct tctccttgcc ttcgagcagg gcggcgagca ggcggtcgtg
     5341 cgcatcgcgc agccgcctga actgatcggc ggcggcgggg tcgggattgc gatcgggatg
     5401 ccatcgcatg gcgagcttgc ggaaggcgcg cttgatctgt tgcgcgctcg cgccggctgg
     5461 caggccgagc agtgcgtggg tgtcggacat ggacgaatgg gtgaggaccg cgagggcatc
     5521 agcttaaccg agcttgcccg cggatgctgc gcccgcactt gacggatctc ccaaggatga
     5581 aagaatgtgg cgccgtgttc aatacggaat cgtatggcct ggtgctccgg cagaactcgc
     5641 cggagcgcga acacgcggaa aatgaataca tggagacgac gaaacgatga gcccaagccg
     5701 agcttccgaa acccagcccc gcgactcgtg ggtgcatcat ccgcaggggc gcatcttcgc
     5761 gcgggaatgg accccgtccg gggcggccgg cgagcggccg gcgagcccga tcgtgctgct
     5821 ccacgactcg ctcggctgtg tcgagctgtg gcgcgacttc ccggcggagc tgagcgccgc
     5881 gagcgggcgc cgggtgatcg cctacgaccg cctcgggttc ggccgctcgg atgcgcgcga
     5941 ggcgctgccg gcgctggact tcatcgccga ggaggcgcag gcgtattttc cggcggtgcg
     6001 cgagcagctc ggcctggagc gcttcgtggt gttcggccac agcgtcggcg gcggcatggc
     6061 ggtgaattgc gcggctgcat ttgccggggc ctgcgacgcc ctgatcaccg agtcggccca
     6121 ggtcttcccc gaggacctca ccctgagcag catcgccgag gccaaggagc agttcgtcga
     6181 tgaatcgcag ctgcagcgcc tggcgcgcta tcacggcgac aaggcgcgct gggtgctgga
     6241 tgcgtggacc gagagctggt tgcatcccgg cttcgcggcc tggtcgctga ggtcggtgct
     6301 gccgcaggtg cgttgccctg tgctggccat ccatggcatg catgacgagt acggcacgag
     6361 cgtgcacccc gagacgatcg gcgagctctc cgcgggcccg gcgcaggtgg cgatcctgcc
     6421 ggacaccttc cacgtgccgc atcgcgagcg tccggccgcg gtcatcgaac tcgtctgcgc
     6481 cttcctcgag ccggtgcggc agggcggggc ggtgtgcgtg tcctgagcgg tcggcgcggt
     6541 gcatccgggc gggtcccgga tgggccgctc gggtatcggg cgggggcgcg ccgatgcgac
     6601 gaggagggac ggaaaaggag gacgaaaaaa aacgccgacc cggtgaagag tcggcgtttt
     6661 tcgaaactgg cgcgcccgga aggattcgaa cctcctaccc cctggttcgt agccaggtac
     6721 tctatccaga tgagctacgg gcgcgttgcc gaaatcgttt agagcccgaa atcggaaggc
     6781 tttttttggc gcgcccggaa ggattcgaac ctcctacccc ctggttcgta gccaggtact
     6841 ctatccagat gagctacggg cgcttcttgc tgcactgcga agaaccggga ttataacgac
     6901 tgttcccgca acttcaagtt taaattggcg gagagggtgg gattcgaacc cacggtaggc
     6961 ttgcacctac gcctgatttc gagtcaggta cattcgacca ctctgccacc tctccgc
//
LOCUS       DAOQBS010000145         6927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_10746_length_6927_cov_16.080908, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000145 DAOQBS010000000
VERSION     DAOQBS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10746_length_6927_cov_16.080908"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PKN25_13230"
     CDS             complement(<1..764)
                     /locus_tag="PKN25_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV91907.1"
                     /translation="MQRPAIIHKTAADLAVPPNLADYAATCAGFSWDAARAELAGLPG
                     GGLNIAHEAVERHAHGPLRERIAFRFLGENSLRDVGYGELSRLTSRFANVLRTLGVRR
                     GERVFVLTGRIPELYIAVLGGLKAGCVVSPLFSAFGPEPIATRIGIGEGAVLVTTDLL
                     YERKVAKLRDRLPSLRHVLLVGEEDGATAIPATQDLATLMAQAADTFETVATRPEDLA
                     LLHFTSGTTGTPKGAMHVHGAVLTHWATGRYALDLH"
     gene            complement(840..968)
                     /locus_tag="PKN25_13235"
     CDS             complement(840..968)
                     /locus_tag="PKN25_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91908.1"
                     /translation="MSGRSEALRRCIHRKGDVETAAMGLLMRGPGFGSMAEAALLA"
     gene            complement(965..2566)
                     /gene="groL"
                     /locus_tag="PKN25_13240"
     CDS             complement(965..2566)
                     /gene="groL"
                     /locus_tag="PKN25_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322108.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNV91909.1"
                     /translation="MTARQLLFRDDARHRILRGVSLLADAVKETLGPRARTVLLQQPY
                     GPPIVINSGVVVARAIELADPFENMGAQLVREVAARTSEVAGDGTTTATLLAAAIVRE
                     GMKHVAAGMNPMDLKRGVDKAVDALVGALKAMARPCGTRTEIAQVASISANNDAAIGE
                     LIADAMERVGKDGVITVEEGSGLASTLDVVEGMQFDRGYLSPYFINTEGARVVLEDAV
                     ILVCDRRIGAVAELLPVLEVVARSGRPLLIIAEEVEGEALALLVVNAARGTLKACAVK
                     APEFGERRRAMLEDIAVLAGATLVGGETGIGLEHATAAELGSARRIEVDKDRCTLVGG
                     GGAHALIEARIARIRAELEGTRSDFEREGPERRIARLAGGVAVVKVGGATESEMKERR
                     ARVEDALHATRAAVAEGILPGGGVALLRARAALGVLHGVNHDEDCGIQIVLRAVEEPL
                     CQLVANGGLEPSVVLDQVAAGSGAFGFNAATEEYGDLIAMGVLDPCKVTRSALQNAAS
                     VAGLILTTDCLVAELPQAGAVAPGG"
     gene            complement(2742..4487)
                     /locus_tag="PKN25_13245"
     CDS             complement(2742..4487)
                     /locus_tag="PKN25_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HNV91910.1"
                     /translation="MRHPLRRGLRFLALALAFAGGGALAQEPLEVRLQAPDEVRPLLE
                     RHVRLLRQEALELPEEQADRSAMTRRARREVAGLLATEGYFSPRVRFDRSDARNWVLE
                     VEPGPRTEIAQVELQFVGEIATSGPGGSEYLDLLRAGWSLPVGAPFSQGGWDGAKQAL
                     LDAVAARRYAAARIVDSRAEIDPESARATLRVRIDSGPSFYLGELDVQGLEHLPTDLV
                     ERYSRLKPGAPYDREELLAFQTGLQNTPHFGSVIVDIERDPTLSAAVPVRVKVTEALP
                     RYAGVGAGYSTNTGARVEFSYRDANLRKRGWEFSSGLRIEERRQALFADVFLPPRGRD
                     RDSFGALYEASDLEGLKIDSQALGAARTTLRGDIETQLALRLQHENIEPAGAPSRSTN
                     TLSANWTWKKRAVDNLLDPSRGYVLEFQVGGGSKTLFSDQDFLRFYSRVARYQPVRGT
                     DVFILRGEAGVTLADSREGVPQDFLFRTGGAQSVRGYDYQSLGVKEGDATVGGRYLAT
                     ASAEYVRWFRPQWGAAVFVDAGDAADTRADYDLRVGYGVGARWRSPAGPLAIDLAWGH
                     QERSLRLHFGVAVAF"
     gene            complement(4504..5505)
                     /locus_tag="PKN25_13250"
     CDS             complement(4504..5505)
                     /locus_tag="PKN25_13250"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNV91911.1"
                     /translation="MRRVTLTQFLIEQQRAGRITADLRLLIEVVARAVKAISVNVSKG
                     ALAGVLGEAGTDNVQGEAQKKLDVIANEILLQANEWGGHLAGMASEEVETICQIPFDL
                     PKGGFLLLFDPLDGSSNIDVNISVGTIFSVLRNPPGGTEPTEESFLQPGREQVAAGYA
                     VYGPATQLVLTVGQGVHGFTLDREMGSFIYTQPFMTIPVDTQEFAINASNARFWEAPV
                     KRYVAEMVQGKEGPLGKDYNMRWVASMVADVHRILTRGGVFMYPMDEKCRSKGGKLRL
                     MYEANPMAMLVEQAGGAASTGRERILDVMPDRLHQRVPVILGSKNEVERVAGYHQED"
     gene            5659..6927
                     /locus_tag="PKN25_13255"
     CDS             5659..6927
                     /locus_tag="PKN25_13255"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HNV91912.1"
                     /translation="MHVMVLGAGLSGVTTAWYLTRAGFQVSLVDRQPGAGLETSFANG
                     GQISISHPEPWANPGAPAQVLRWLGRADAPLKFRPHRALAQWTWGLRFLLECLPARAH
                     RNTVAIAALALASGQRLRALRAELGLEYDQLERGILHLFYSRGEFAKAPARAAQLRAF
                     GIAAEVLDRDACLEREPALATCAGSLAGGIFAQGDESGDAHKFTRALAHRLEEGGVVL
                     HRETEVSALETGSGGRVVGARVRRADGSVDSLRADACVLCLGSHGPRLAAPLGLRLPI
                     YPVKGYSITAPVLEPALAPYVSLTDESRRIVCSRLGDRLRVAGTAELDGFDTTIRAAR
                     IAPIMAWVQARFPHAIDLERAEPWAGLRPATPGNVPLIGRSARPGLWLNTGHGTLGWT
                     LACGSAAALADLMSGRRPEVAFPFLAAAEV"
BASE COUNT          983 a         2549 c         2411 g          984 t
ORIGIN      
        1 tcatgcagat cgagcgcgta gcggccggtc gcccagtggg tgaggaccgc gccatgcacg
       61 tgcatggcac ccttgggcgt gccggtggtg ccgctggtga agtgcagcag ggcgaggtct
      121 tcgggccggg tcgccaccgt ctcgaacgtg tccgcggcct gcgccatcag cgtggcgagg
      181 tcctgggtgg cggggatggc cgtcgcgcca tcctcctcac ccaccagcag gacatggcgc
      241 aggctcggca ggcgatcacg cagcttcgcc accttgcgct cgtacagcag gtcggtggtg
      301 accagcaccg ccccctcgcc gatgccgatc cgggtggcga tcggctccgg gccgaaggcg
      361 gagaacagcg gcgacaccac gcagcccgcc ttcaggccgc cgagcaccgc gatgtacagc
      421 tccgggatgc gcccggtcag cacgaagaca cgctcgccgc gccgcacgcc cagggtccgc
      481 agcacgttgg cgaagcggct ggtgaggcgc gacagctcgc cgtagccgac atcacggagc
      541 gagttctcac cgaggaaacg gaaggcgatg cgctcgcgca gcgggccatg cgcgtggcgc
      601 tcgaccgcct catgggcgat gttgagcccg ccgccgggca gccctgccag ctcggcgcgc
      661 gccgcgtccc aggagaagcc ggcacaggtc gcggcgtagt cggcaagatt gggcggcacg
      721 gccagatcgg cggccgtctt gtggatgatc gcggggcgct gcatcgcctt ctcctcacca
      781 tcgtcttcat cgtcgcatgg cagcgacggc ttcgcgtcgg ccacctcccc ggtttctttt
      841 taggccagca gggccgcctc ggccatgctg ccgaagcccg ggccgcgcat gaggaggccc
      901 atcgccgccg tttcgacgtc ccccttgcgg tgaatgcagc ggcgcagcgc ctcgctacgg
      961 ccgctcaccc gcccggcgca acagccccgg cttgcggcag ctcggccacc aggcaatcgg
     1021 tggtgaggat caggcccgcc accgaggccg cattctgcag cgcactgcgc gtgaccttgc
     1081 acgggtcgag cacgcccatc gcgatgaggt cgccgtattc ctcggtcgcg gcattgaagc
     1141 cgaacgcgcc gctgcccgcc gccacctggt ccagcaccac ggaaggttcc aggccgccgt
     1201 tggcgacgag ctggcacagc ggctcctcca ccgcgcgcag cacgatctgg atgccgcagt
     1261 cctcgtcgtg gttgacgcca tgcagcacgc ccagcgccgc gcgcgcgcgc aacagcgcca
     1321 cgccgccgcc gggcaggatg ccttcggcca ccgcggcgcg tgtcgcatgc agcgcgtcct
     1381 cgacacgcgc acggcgctcc ttcatctcgg actcggtggc cccacccacc ttcaccaccg
     1441 ccacgccgcc ggccaggcgc gcgatgcgcc tctccgggcc ctcgcgctcg aagtcggagc
     1501 gcgtgccctc gagctcggcg cggatgcggg cgatgcgggc ctcgatcagc gcgtgcgcgc
     1561 cgccgccacc caccagcgtg cagcggtcct tgtccacctc gatgcggcgc gcgctgccca
     1621 gctcggccgc ggtggcgtgt tccaggccga tgccggtctc gccgccgacc agcgtggcgc
     1681 cggccagcac cgcgatgtcc tcgagcatgg cgcggcggcg ctcgccgaac tccggcgcct
     1741 tcaccgcgca ggccttcagc gtgccgcgcg cggcgttcac caccagcagc gccagcgcct
     1801 cgccctcgac ttcctcggcg atgatcagca agggccgccc gctgcgcgcc acgacctcga
     1861 gcaccggcag cagctcggcc acggcgccga tcctgcggtc gcacacgagg atgacggcat
     1921 cctcgagcac gacgcgcgca ccctcggtgt tgatgaaata gggcgacagg taaccacggt
     1981 cgaactgcat gccctcgacc acgtccagcg tgctcgcgag gcccgagccc tcctccacgg
     2041 tgatcacgcc gtccttgccg acgcgctcca tggcgtcggc gatcagctcg ccgatggcag
     2101 cgtcgttgtt ggccgagatg ctcgccacct gggcgatctc ggtgcgcgtc ccgcacggcc
     2161 gcgccatcgc cttcagcgcg ccgacgagcg cgtcgacagc cttgtcgacg ccgcgcttca
     2221 gatccatcgg gttcatgccc gcggcgacgt gcttcatgcc ctcgcgcacg atggccgcag
     2281 ccagcagggt cgcggtggtg gtgccgtcgc ccgccacttc ggaagtgcgc gccgccacct
     2341 cccgcaccag ttgggcgccc atgttctcga agggatcggc gagctcgatc gcgcgcgcga
     2401 ccacgacccc ggagttgatc acgatcggcg gcccataggg ctgctgcagc agcacggtgc
     2461 gcgcgcgcgg ccccagcgtc tccttcaccg catcggccag cagggacacg ccgcgcagga
     2521 tgcggtggcg cgcgtcgtcg cggaaaagca gttgtctggc ggtcatcgtc tcctccctgt
     2581 tcggcctcgc cgcgcggatc accgtgaaaa tctactcctg ttccgcgggc aatggcgagg
     2641 cgaccagggg ggcgagcaga acgcggacgg cgcgggggcg aagggcagcg ctgcgcgcgc
     2701 agacgccgat cggccgatgg cagcccatcg agcgccgcgc ctcagaacgc caccgcgacc
     2761 ccgaaatgca gccgcaggct gcgttcctgg tggccccagg cgagatcgat cgcgagcggg
     2821 cccgccgggc tgcgccagcg cgcgccgacg ccgtagccga cgcgaagatc gtagtccgcg
     2881 cgggtatcgg cggcatcccc cgcatcgacg aagacggccg caccccactg cggccggaac
     2941 cagcgcacgt actcggcgct cgcggtcgcc aggtagcggc cgccgacggt cgcgtcgccc
     3001 tccttcacgc ccaggctctg gtagtcgtag ccgcgcaccg actgcgcgcc gccggtgcgg
     3061 aagaggaagt cctgcggcac gccctcgcgg ctgtcggcca gggtcacgcc agcctcgccg
     3121 cgcaggatga acacgtcggt gccgcgcacc ggctggtagc gcgcgacgcg cgagtagaaa
     3181 cgcaggaagt cctggtcgga gaatagcgtc ttgctgcccc cgccgacctg gaactcgagc
     3241 acgtagccgc ggctggggtc gagcaggttg tcgaccgcgc gcttcttcca ggtccagttg
     3301 gcgctcaggg tgttggtcga gcgcgagggc gcgccggccg gttcgatgtt ctcgtgctgc
     3361 aggcgcagcg cgagctgggt ctcgatgtcg ccgcgcagcg tggtgcgcgc ggcgccgagc
     3421 gcctgcgagt cgatcttgag cccttcgagg tcgctggcct cgtagagcgc gccgaagctg
     3481 tcgcggtcgc gcccgcgcgg cggcaggaag acgtcggcga acagcgcctg gcggcgctcc
     3541 tcgatgcgca ggccggagga gaactcccag ccgcgcttgc gcaggttggc gtcgcgatag
     3601 ctgaactcga cgcgcgcgcc ggtgttggtc gaatagcccg cccccacccc ggcatagcgc
     3661 ggcagtgctt cggtgacctt gacccgcacc ggcaccgctg cggacagcgt cgggtcgcgc
     3721 tcgatgtcga cgatcaccga accgaagtgc ggggtgttct gcagtccggt ctgaaaggca
     3781 agcagctcct cgcggtcgta gggcgcaccg ggcttgagcc ggctgtagcg ctcgacgaga
     3841 tcggtcggca gatgctccag gccctgcacg tcgagctcgc caaggtagaa gctcggtccg
     3901 ctgtcgatgc gcacccgcag cgtggcacgc gcgctctccg ggtcgatctc ggcgcggctg
     3961 tccacgatgc gcgccgcggc gtaacggcgc gccgcgaccg catcgagcag agcctgcttg
     4021 gcgccgtccc agccgccctg gctgaagggc gcgccgaccg gcaggctcca gcccgcgcgc
     4081 agcaggtcga ggtactcgct gccgcccggc ccggaggtgg cgatctcgcc gacgaactgc
     4141 agctcgacct gcgcgatctc ggtgcgcgga ccgggttcca cctccagcac ccaattgcgc
     4201 gcgtcgctgc gatcgaagcg cacccgcggc gagaagtagc cctcggtggc gagcaggccc
     4261 gccacctcgc gacgcgcccg ccgggtcatc gccgagcggt cggcctgctc ctcgggcagt
     4321 tcgagcgcct cctggcgcag caggcgcacg tggcgctcga gcagcggtcg cacctcgtcg
     4381 ggcgcctgca ggcgcacctc caggggctcc tgcgccagcg cgccaccccc cgcgaacgcg
     4441 agggccagcg cgaggaagcg cagcccccgg cgcagcgggt gtcgcactcc cgagcgcacc
     4501 cgctcagtcc tcctggtggt agcccgcgac gcgctcgacc tcgttcttcg agccgaggat
     4561 caccggcacg cgctggtgca gccggtccgg catcacgtcg aggatgcgct cgcgcccggt
     4621 ggaggccgcg ccgccagcct gctcgaccag catcgccatc gggttggcct cgtacatcag
     4681 gcgcagcttg ccgcccttgc tgcgacactt ctcgtccatc gggtacatga acacgccgcc
     4741 gcgggtgagg atgcgatgca cgtctgcgac catcgaggcc acccagcgca tgttgtagtc
     4801 cttgccgagc ggaccttcct tgccctgcac catctcggcg acgtagcgct tgaccggcgc
     4861 ctcccagaag cgcgcattgg atgcgttgat ggcaaattcc tgggtatcga ccgggatggt
     4921 catgaagggc tgggtgtaga tgaagctgcc catctcgcgg tcgagcgtga atccgtgcac
     4981 gccctgcccc acggtgagca cgagctgggt ggcaggacca tacacggcgt agccggccgc
     5041 gacctgctcg cgaccgggct gcaggaagct ctcctcggtg ggctccgtgc cgcccggcgg
     5101 gttgcgcagc accgaaaaga tggtgccgac cgagatgttg acgtcgatgt tggacgagcc
     5161 gtcgagcgga tcgaagagca gcaggaagcc gcccttgggc aggtcgaagg ggatctggca
     5221 gatggtctcg acttcctccg aggccatgcc ggcgagatgg ccgccccact cgttggcctg
     5281 cagcaggatc tcgttggcga tgacgtcgag cttcttctgc gcctcgccct ggacgttgtc
     5341 ggtgccggcc tcgccgagca cgccggcgag cgcgcccttg gagacgttga cgctgatcgc
     5401 cttgaccgcg cgagcgacga cctcgatcag caggcgcagg tcggcggtga tgcggccggc
     5461 gcgctgctgc tcgatgagga actgggtaag ggtgactcgg cgcatgacaa ggctccacga
     5521 cattgaacga aacggacaag gcgggattat cgcggttccg cgccacgagc gcacgggccg
     5581 tgatgcccga aaccgcgaga ctgctgccgc agccgccgag gcgctatcat cggcaccccc
     5641 aacccgcatt tccacttcat gcacgtcatg gttctcggcg ccggcctgtc cggcgtcacg
     5701 accgcctggt acctgacccg cgccggcttc caggtcagcc tggtcgaccg ccagcccggc
     5761 gccggactgg agaccagctt cgccaacggc gggcagatct cgatcagcca tcccgagccc
     5821 tgggccaacc ccggcgcacc cgcgcaggtg ctgcgctggc tcggacgcgc ggacgcgccg
     5881 ctgaagttcc gcccgcaccg cgcccttgcg cagtggacct ggggcctgcg tttcctgctc
     5941 gaatgcctgc ccgcacgggc gcaccgcaac accgtcgcga tcgccgcgct cgcgctcgcc
     6001 agcgggcagc gactgcgtgc gctgcgcgcc gaactcggcc tcgaatacga ccagctcgag
     6061 cgcggcatcc tgcacctgtt ctacagccgc ggggagttcg ccaaggcccc cgcgcgcgct
     6121 gcgcagctgc gcgccttcgg tatcgcggcc gaggtgctgg accgcgatgc ctgcctggaa
     6181 cgcgagcccg cgctggccac gtgcgccggc agcctggccg gcgggatctt cgcccagggc
     6241 gacgaatcgg gcgacgcgca caagttcacc cgcgcgctcg cgcacaggct ggaagaaggt
     6301 ggtgtggtcc tgcaccggga gaccgaggtg agcgcgctcg agaccggatc cggcggccgg
     6361 gtcgtgggcg cgcgcgtgcg ccgcgcggac ggcagcgtcg acagcctgcg cgcggacgcc
     6421 tgcgtgctct gcctgggcag ccacggcccg cgcctcgccg cgccgctcgg cttgcggctg
     6481 ccgatctacc cggtcaaggg ctactcgatc accgcaccgg tactcgagcc cgcgctcgca
     6541 ccctacgtga gcctgaccga cgagtcgcgc cggatcgtgt gctcgcgcct gggcgaccgc
     6601 ctgcgcgtcg ccggcacggc ggaactcgac ggcttcgaca ccacgatccg cgcggcgcgg
     6661 atcgcgccga tcatggcgtg ggtgcaggcg cgcttcccgc acgcgatcga tctcgaacgc
     6721 gcggaaccct gggccgggtt gcgccccgcc acgccgggaa acgtgccact gatcggacgc
     6781 agcgcgcgcc ccgggctatg gctcaacacc ggccatggca cgctgggatg gacgctggcc
     6841 tgcggttcgg ccgccgcact ggcggatctg atgagcgggc gcaggccgga ggtggcgttt
     6901 cctttcctcg cggcggcgga ggtctag
//
LOCUS       DAOQBS010000146         6731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_11210_length_6731_cov_12.268574, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000146 DAOQBS010000000
VERSION     DAOQBS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11210_length_6731_cov_12.268574"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PKN25_13260"
     CDS             <1..174
                     /locus_tag="PKN25_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV91913.1"
                     /translation="PSTISFGTMINMARAELAREPMVWWSLAAAFVFMFVLVLAANLF
                     ADVVRDAFDPRRA"
     gene            224..1798
                     /locus_tag="PKN25_13265"
     CDS             224..1798
                     /locus_tag="PKN25_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNV91914.1"
                     /translation="MRALRFDNRFVRELPADPEAENHVRPVHGACYSRVMPTPVRAPR
                     LLAWSREVAHILGLEEADVRSAEFARVFGGNGLLPGMEPYAACYGGHQFGNWAGQLGD
                     GRAITLGESINARGERWELQLKGAGPTPYSRFADGRAVLRSSLREFLCSEAMHHLGVP
                     TTRALSLVGTGETVVRDMLYDGNPRPEPGAVVCRVAPSFIRFGNFEIFASRGEEALLE
                     RLIDFTIARDFPELAAEPDAAARRIRWFDEVCRRTAVLVAHWMRVGFVHGVMNTDNMS
                     ILGLTIDYGPYGWVDDFDPDWTPNTTDAGGRRYRFGNQPFIAHWNLWQLANAIYPVVR
                     EVEPLERALAAYADVHDSSYRDMMRAKLGLAEWRGGEEGDDGLLERLHRLLAAGEVDM
                     SLFFRRLADVDPTAPTLEPLAEAFYDPTRRAAVEAELLAWLRAHGARVLGDGRLAAAR
                     RAEMNRVNPLYVPRNYLAQQAIDAAEGGDMSELEALLEVLRRPYDEQPGRERFAARRP
                     DWARDRPGCSMLSCSS"
     gene            2042..2764
                     /locus_tag="PKN25_13270"
     CDS             2042..2764
                     /locus_tag="PKN25_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNV91915.1"
                     /translation="MRPALARKAASPREALARMPILLGSGPEVLDELARGAEFVRLSS
                     SATVFERGSHPSGLFFVCNGAVRLMVSGPEGKDKVVELFEPGGMFGEIGVFTGERYRT
                     WTQAVGAVVLVHVPRERVLAAVAMDNSLSNRMLVAVCARIQRLIDAIGSTVGGPAAVR
                     VASYLLEQLERGARSDGCIVLAAPKKTIASLLNLTPETLSRVLRSLVEEGVLTVSGRR
                     IRVRNRAQLARLLTHDEAVLPL"
     gene            2915..4177
                     /locus_tag="PKN25_13275"
     CDS             2915..4177
                     /locus_tag="PKN25_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HNV91916.1"
                     /translation="MATRKFQQMSVLIMSTTAFTVCFAVWMLFAVIGIPIKEVLALNE
                     TQFGLLAATPVLTGSLIRLPLGMLTDKFGGRIVFFVLMLCTVLPIWLIGHATEYWQFL
                     VAGLFVGFAGGSFSVGIAYCARWFERQNQGFAMGVFGAGNSGAAVTKFVAPTLVVMFG
                     WQMVPQVYAAAMLITAVAFWFFTYDDPEHRVHSSVTFRDQLRVLKDPRVWKYCQYYSI
                     VFGGYVGLSLWLTKYYVNEFGMGIQAAALLAACFSLPGGVLRAVGGWLSDRYGAHKVT
                     WWVMWASWICLFLLSYPNTDFTVQTVEGPRTFSIHLNVWVFTILLFIVGIAFAFGKAS
                     VFKYISNEFPKEIGAVSGIVGLVGGMGGFLLPIMFGALVDLTGVRSSAFMLLYGVTWV
                     SLIWMYLTEVRRVDMFDGHDTAVDSSKA"
     gene            4205..5860
                     /locus_tag="PKN25_13280"
     CDS             4205..5860
                     /locus_tag="PKN25_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV91917.1"
                     /translation="MQASNVAAPSPAITSSVDLVDWRPEDEAFWKEKGSRIANRNLWI
                     SIPSLLCGFAVWLMWGIITVQMANAGFPFAPDQLFSLAAIAGLSGATTRIPASFFIRI
                     CGGRNTVWLTTALLMIPAVGTGIALQSLETPLWVFQLMALLSGMGGGNFACSMANIST
                     FFPKRLQGTALGLNAGLGNFGVTTSQLLIPAVMTMGVFGALGGDAIPLVKDSATLIGK
                     IAAGTPTYVQNAGFVWLLLLVPLAVVGWFGMNNLLTVSPNIGSTGAALGKIILFYGIA
                     LVVSAIGLYLYLPSGLGVLAGGGMWIMLPLVILGTLYGMKFAASGEMKTNLDKQFAIF
                     RNKHTWSMTALYVVTFGSFIGFSMALPLSITVIFGNTHVLDPATQAWVHAKNPNAPSA
                     LMFAWIGPFVGALIRPVGGWISDKLGGSIVTQWISIVLVIASAVTGYVMFLAYHSATP
                     EQYFFMFMALFVLLFAASGIGNGSTFRTVGFVFDKDERGPVLGWTSAVAAYGSFIAPV
                     VIGGQIKAGTPEYAMYGFAVFYALCVVLNWWFYLRKDAYIQNP"
     gene            5950..>6731
                     /locus_tag="PKN25_13285"
     CDS             5950..>6731
                     /locus_tag="PKN25_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV91918.1"
                     /translation="MSHFLDRLRFFDKVKETFSNGHGIVTNEDRNWEDAYRNRWRHDK
                     VVRSTHGVNCTGGCSWKIFVKNGLVSFEMQQTDYPRTREDLPNHEPRGCQRGASFSWY
                     LYSPHRIKYPMIRGRLLDLYRAERKAGRDPVEAWEAIQKDPAKREKYVHVRGLGGFVR
                     TSWDEVTEIVAAANVYTIKKWGPDRIYGFSPIPAMSMISYAAGARYLSLIGGACGSFY
                     DWYCDLPAASPQTWGEQTDVPEAADWYNSTYLIITGANLPMTR"
BASE COUNT          957 a         2319 c         2219 g         1236 t
ORIGIN      
        1 ccgtcgacga tcagcttcgg caccatgatc aacatggcgc gtgccgaact cgcgcgcgag
       61 ccgatggtct ggtggtcgtt ggcggcggcc ttcgtgttca tgttcgtgct cgtgctcgcc
      121 gccaacctgt tcgccgacgt ggtgcgcgac gccttcgacc cgcgccgcgc ctgagcgcgc
      181 cctggcccgc acccgccggg ccgaccatga cgaggatttc ccgatgagag cgcttcgctt
      241 cgacaaccgc ttcgtgcgcg agctgcccgc cgatcccgaa gcggagaacc acgtccgtcc
      301 ggtccatggc gcctgctact cgcgggtgat gccgacgccg gtgcgggcgc cgcgcctgct
      361 ggcgtggtcg cgcgaggtgg cgcacatcct gggcctggag gaggccgacg tgcgcagcgc
      421 cgagttcgcc cgcgtcttcg gcgggaacgg cctgctgccc ggcatggagc cctacgccgc
      481 ctgctacggc ggccaccagt tcggcaactg ggcggggcag ctcggtgacg ggcgcgcgat
      541 cacgctcggc gagtcgatca atgcgcgcgg cgaacgctgg gagctgcagc tcaagggcgc
      601 cgggccgacg ccgtattcgc gcttcgccga cggccgcgcg gtgctgcgct cctcgctgcg
      661 cgagttcctg tgcagcgagg cgatgcacca cctgggcgta ccgaccacgc gcgcgttgag
      721 cctggtggga accggcgaga ccgtggtgcg cgacatgctc tacgacggca atccccgacc
      781 cgagccgggc gcggtggtgt gccgggtggc gccgtccttc atccgcttcg gcaacttcga
      841 gatcttcgcc tcgcgcggcg aggaggcgct gctcgagcgc ctgatcgact tcacgatcgc
      901 gcgcgatttt cccgaactcg ccgccgagcc cgatgcggcg gcgcggcgca tccgttggtt
      961 cgacgaggtc tgccggcgca ccgcggtgct ggtcgcgcac tggatgcggg tgggcttcgt
     1021 gcatggggtc atgaacaccg acaacatgtc catcctcggc ctcaccatcg attacggccc
     1081 ctacggctgg gtcgacgact tcgaccccga ctggacgccc aataccaccg acgccggcgg
     1141 gcgccgctac cgcttcggca accagccgtt catcgcgcat tggaacctgt ggcagctcgc
     1201 caacgcgatc tatccggtgg tgcgcgaggt cgagccgctg gagcgcgcgc tcgccgcgta
     1261 cgcggacgtc catgattcga gctatcgcga catgatgcgc gccaagctcg gcctcgccga
     1321 atggcgtggt ggcgaggagg gcgacgacgg cctgctcgag cgcctgcacc gcctgctcgc
     1381 ggccggcgag gtggacatga gcctgttctt ccgccgcctc gccgacgtcg atccgacggc
     1441 gcccacgctg gagccgctcg ccgaggcctt ctacgacccc acgcgccgcg ccgcggtgga
     1501 ggcggagctg ctcgcctggt tgcgagcgca tggcgcgcgc gtgctcggcg acggtcggct
     1561 ggccgcggcg cgacgggcgg agatgaaccg cgtcaatccg ctctacgtgc cgcgcaacta
     1621 cctggcccag caggcgatcg acgccgcgga gggtggggac atgagcgagc tcgaggccct
     1681 gctcgaggtg ctgcgccgac cctacgacga gcagcccggg cgcgagcgct tcgcggcgcg
     1741 ccggccggac tgggcgcgcg atcgccccgg ctgctccatg ctctcctgca gttcctgaac
     1801 gtgcaggcgg catcgcgcgt ggtcgtcgcc gggcgtgcct gcgccatccg ggttcgccgc
     1861 ttcccgaggg ctccttgagg cgagggtagt cctttcggcg tagcgctccg tcgatcggcc
     1921 aatggccagc ggccgtgcca gggtctagga tggaactgga ggaggaaccg tcgcgaccca
     1981 tgccggcgga taaaacagac ctgtgtccac catcgtcctg actcatgcgg aggagtcgcg
     2041 cgtgcgtcct gcgctcgcgc gcaaggccgc ttcgcctcgc gaggccttgg cgcgcatgcc
     2101 gatcctgctc ggcagcgggc ccgaggtgct cgacgagctt gcgcgcggcg cggagttcgt
     2161 ccgcctgtcc tcgtcggcca cggtgttcga gcgcggctcc caccccagcg gcctgttctt
     2221 cgtctgcaac ggcgcggtcc gcctgatggt cagcgggccc gagggcaagg acaaggtggt
     2281 cgagctcttc gagccgggcg gcatgttcgg cgagatcggc gtgttcaccg gcgagcgcta
     2341 tcgaacgtgg acgcaggcgg tcggcgcggt cgtgctggtg catgttccgc gcgagcgcgt
     2401 gctggccgcg gtggcgatgg acaattcgct ctccaaccgc atgctggtcg cggtgtgcgc
     2461 gcgcatccag cgcctgatcg acgcgatcgg cagcaccgtc ggcggcccgg cggcggtgcg
     2521 cgtggcgagc tatctgctcg aacagctcga gcgcggtgcg cgcagcgatg gttgcatcgt
     2581 cctggccgcg cccaagaaga ccatcgcctc cttgctcaac ctcacgcccg agacgctctc
     2641 gcgggtgttg cgcagcctgg tcgaggaggg cgtgctcacc gtgagcggcc gtcgcatccg
     2701 cgtgcgcaac cgtgcgcagc tcgcccgcct gctgacgcac gacgaggcgg tcctacccct
     2761 ctgagctcgt ccgggctagt cactaaggac tagtccaccc tcgtgccggc agcgccgatc
     2821 ccgtgctttt ggcgaatggt gcggggcgcc gcccgagcct atcctcaccc cggaaaattc
     2881 gtgaccgtcg gacctttccg gagcgcagtg ggaaatggct actcgcaagt ttcagcagat
     2941 gtccgtgctc atcatgagca ccaccgcatt caccgtctgt tttgccgtgt ggatgctgtt
     3001 cgcggtgatc ggtatcccga tcaaggaggt tctcgccctc aacgagaccc agttcggcct
     3061 gctggcggct accccggtgc tgaccggatc gctgatccgt ctgcccctcg gcatgctcac
     3121 cgacaagttc ggcggccgca tcgtcttctt cgtgctgatg ctgtgcaccg tgctgccgat
     3181 ctggctgatc ggccatgcga ccgaatactg gcagttcctc gtcgccggcc tcttcgtcgg
     3241 cttcgcgggc ggctccttct cggtcggcat cgcctactgc gcgcgctggt tcgaacgcca
     3301 gaaccagggc ttcgcgatgg gggtgttcgg tgccggcaac tcgggcgccg cggtcaccaa
     3361 gttcgtcgcg cccacgctgg tggtcatgtt cggctggcag atggtgccgc aggtctatgc
     3421 ggcggcgatg ctgatcaccg cggtcgcgtt ctggttcttc acctacgacg accccgagca
     3481 ccgggtgcac tcctcggtga ccttccgcga ccagctgcgc gtgctcaagg atccgcgcgt
     3541 gtggaagtac tgccagtact actcgatcgt gttcggcggc tacgtcggcc tgtcgctgtg
     3601 gctcaccaag tactacgtca acgagttcgg catgggcatc caggcggcgg cgctgctcgc
     3661 tgcctgcttc tccctgcccg gcggcgtgct gcgcgcggtg ggcggctggc tgtccgaccg
     3721 ctacggcgcg cacaaggtca cctggtgggt gatgtgggcg tcgtggatct gcctgttcct
     3781 gctctcctat ccgaacaccg acttcaccgt gcagaccgtc gaggggccgc gcaccttcag
     3841 catccacctc aacgtgtggg tgtttacgat cctgctcttc atcgtcggca tcgccttcgc
     3901 cttcggcaag gcctcggtgt tcaagtacat ctccaacgag ttccccaagg agatcggcgc
     3961 ggtctccggc atcgtcggcc tggtgggcgg catgggcggc ttcctgctgc cgatcatgtt
     4021 cggcgccctg gtcgacctca ccggcgtgcg ctcgagcgcc ttcatgctgc tctacggcgt
     4081 gacctgggtt tccctgatct ggatgtacct gacggaagtc cgtcgtgtcg acatgttcga
     4141 cggtcacgac accgcggtcg actcgtccaa ggcctgattc gcaagacaac gaggagtggg
     4201 tttcatgcaa gcaagcaatg tcgcggcgcc gagcccggcg atcaccagca gcgtcgacct
     4261 cgtcgattgg cgccccgagg acgaggcctt ctggaaggag aagggctcgc ggatcgccaa
     4321 ccgcaacctg tggatctcca tccccagcct gctgtgcggc ttcgccgtgt ggctgatgtg
     4381 ggggatcatc acggtgcaga tggccaacgc gggcttcccc ttcgcccccg accagctctt
     4441 ctcgctcgcc gcgatcgccg gcctgtcggg tgccaccacg cgcatcccgg cctccttctt
     4501 catccgcatc tgcggcggtc gcaacacggt gtggctgacc accgcgctgc tgatgatccc
     4561 cgccgtgggt accggcatcg cgctgcagag tctggagacg ccgctgtggg tgttccagct
     4621 catggcgctg ctgtcgggca tgggcggcgg caacttcgcc tgctcgatgg ccaatatctc
     4681 caccttcttc cccaagcgcc tgcagggcac cgcgctcggc ctcaacgccg gcctgggcaa
     4741 cttcggcgtg accacctcgc agctgctgat tccggcagtg atgaccatgg gggtgttcgg
     4801 cgcactcggc ggcgacgcga tcccgctggt caaggattcc gccacgctga tcggcaagat
     4861 cgccgccggc acgccgacct acgtgcagaa cgccggcttc gtctggctgc tgctgttggt
     4921 gccgctcgcg gtggtcggct ggttcggcat gaacaacctc ctcaccgtgt cgcccaacat
     4981 cggctccacg ggcgcggcgc tcggcaagat catcctgttc tacggcatcg ccctggtggt
     5041 ttcggcgatc ggtctctacc tctacctgcc gagcggcctc ggcgtgctcg cgggcggcgg
     5101 gatgtggatc atgctgccgc tggtcatcct cggcacgctg tacggcatga agttcgccgc
     5161 cagcggcgag atgaagacca atctcgacaa gcagttcgcg atcttccgca acaagcacac
     5221 ctggtcgatg accgcgctct acgtggtgac cttcggctcc ttcatcggtt tctcgatggc
     5281 gctgccgctg tcgatcacgg tgatcttcgg caacacccac gtgctcgatc cggccaccca
     5341 ggcctgggtg catgcgaaga acccgaacgc gccctccgcg ctgatgttcg cctggatcgg
     5401 ccccttcgtc ggcgcgttga tccgaccggt gggcggctgg atctcggaca agctgggcgg
     5461 ctcgatcgtg acccagtgga tctcgatcgt gctcgtgatc gcctccgcgg tgaccggcta
     5521 cgtgatgttc ctcgcctacc actcggcgac gccggaacag tacttcttca tgttcatggc
     5581 gctgttcgtg ctgctcttcg cggcctccgg catcggcaac ggctcgacct tccgtaccgt
     5641 gggcttcgtg ttcgacaagg acgagcgcgg ccccgtgctg ggctggacct ctgcggtggc
     5701 ggcctacggc tccttcatcg ctccggtggt gatcggcggc cagatcaagg ccggcacgcc
     5761 cgagtacgcg atgtacggct ttgcagtgtt ctacgccctg tgcgtggtcc tcaactggtg
     5821 gttctacctg cgcaaggacg cgtacatcca gaacccctga ttcccgcccg accgacggat
     5881 tccctttttc aaccagactt ttaccgatcg cgcttgacga acgaagcgac gacttgagag
     5941 gagcaaacca tgagtcattt tctcgatcgc ctgaggtttt tcgacaaggt caaggagacc
     6001 ttctcgaacg gacacggtat cgtcaccaat gaagaccgca actgggagga tgcctaccgc
     6061 aaccgctggc gccacgacaa ggtggtgcgc tccacccacg gtgtgaactg caccggcggc
     6121 tgttcctgga agatcttcgt caagaacggc ctggtgtcgt tcgagatgca gcagaccgac
     6181 tacccgcgca cccgcgagga cctgcccaac cacgagccgc gcggctgcca gcgtggtgcc
     6241 tcgttctcgt ggtacctgta ctcgccgcac cgcatcaagt acccgatgat ccgcggccgc
     6301 ctgctcgacc tgtaccgcgc cgagcgcaag gccggacgcg acccggtcga ggcctgggag
     6361 gcgatccaga aggatcccgc caagcgcgag aagtacgtgc acgtgcgcgg cctgggtggc
     6421 ttcgtgcgca ccagctggga cgaggtcacc gagatcgtcg ccgcagccaa cgtctacacg
     6481 atcaagaagt ggggcccgga ccgcatctac ggtttctcgc cgattcccgc gatgtcgatg
     6541 atctcgtacg ccgccggcgc gcgttacctg tcgctgatcg gtggtgcctg cggctcgttc
     6601 tacgactggt actgcgacct gccggccgcg tcgccgcaga cctggggcga gcagaccgac
     6661 gtgcccgagg ccgccgactg gtacaactcg acctatctga tcatcaccgg cgccaacctg
     6721 ccgatgaccc g
//
LOCUS       DAOQBS010000147         6709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_11280_length_6709_cov_15.723775, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000147 DAOQBS010000000
VERSION     DAOQBS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11280_length_6709_cov_15.723775"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /gene="rph"
                     /locus_tag="PKN25_13290"
     CDS             complement(<1..125)
                     /gene="rph"
                     /locus_tag="PKN25_13290"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586293.1"
                     /note="RNase PH; tRNA nucleotidyltransferase; forms
                     hexamers in Bacillus subtilis; phosphoroltic 3'-5'
                     exoribonuclease; involved in maturation of tRNA precursors
                     and removes terminal nucleotides near CCA acceptor arms of
                     mature tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNV91919.1"
                     /translation="MRPSLRQPDQLRAVRLTRGFTCHAEGSVLVEFGATRVLCTA"
     gene            complement(135..1058)
                     /locus_tag="PKN25_13295"
     CDS             complement(135..1058)
                     /locus_tag="PKN25_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HNV91920.1"
                     /translation="MRFTIYQESRPGKRRDNQDRIAHCYSRDALLMLVADGMGGHLHG
                     EVAAQIAVQCIAERFQAEARPLLPDPQLFLARAIERAHHAILDHALDRQLPEAPRTTL
                     VACVIQRGAAYWAHAGDSRLYLLRRGHLVTRTRDHSRTQLMIDQGVIKPEEAHLHPGR
                     NRIYSCLGGNYNPQVEYSPRTPLRDGDLLALCTDGVWGPIDDDGLVVNLTAGVLSRAV
                     PRLMDVAERKAGASADNLSMVAMCWHDENAVGAATVESISTRTMELDGFESRLDLSGP
                     GPTAVAELDEDEIERAIREINAAIHKFDSRS"
     gene            complement(1068..2015)
                     /locus_tag="PKN25_13300"
     CDS             complement(1068..2015)
                     /locus_tag="PKN25_13300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNV91921.1"
                     /translation="MPSQANCALPPGFQLDQYRIERQLSLGGFSIVYLARDAHGMAVA
                     IKEYLPNSLALRKEGETEPQVSEEHRLAFRYGMKCFFEEGRSLAKLMHPNVVRVLNFF
                     RANGTVYMVMQFERGRTLHDYIRKHRGEVKEMLIRAVFARMLNGLREVHAHKLMHLDI
                     KPSNIYLRNDGTPVLLDFGAARQTLMSDQPVLKPMYTPGYAAPEQYEKGAQLGPWTDI
                     YSVGASLHACIVGSPPPRADERAREDTLQPLARTQAGRYSRQLLELVDWCLHLDPQQR
                     PPSVYALQKALIHQENAPERPEHWFSDLGSRLRSFIGRS"
     gene            2107..>2591
                     /locus_tag="PKN25_13305"
     CDS             2107..>2591
                     /locus_tag="PKN25_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91922.1"
                     /translation="MVQSMTGFAVQTRDLGSVSLHLELRSVNSRYLDLGFRIVDDLRA
                     AEPAIRERISARLGRGKVECRLNLQAGHAAPRSMALNGALLDQLAAAQDGLRARFPDA
                     APLSVNELLRWPGMLADDSLGFDEMLPAIVELTNTALDELVATRQREGDKLADMIRER
                     VA"
     assembly_gap    2592..2691
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2692..3128
                     /locus_tag="PKN25_13310"
     CDS             <2692..3128
                     /locus_tag="PKN25_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNV91923.1"
                     /translation="TRQREGDKLADMIRERVARMRELVAQAAPRMPAVVLEYQERLTA
                     KLRDAVASLDEDRVRQEVALYAQRIDVDEELTRLSTHLDEVERILKSAGAAGKRLDFL
                     MQELNREANTLASKSPATDITAIAMEMKVLIEQMREQVQNLE"
     gene            3345..4673
                     /locus_tag="PKN25_13315"
     CDS             3345..4673
                     /locus_tag="PKN25_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNV91924.1"
                     /translation="MTTSTWSASGAAAFAACVAAAATPAFAAAPADWAAIPVQTVTLF
                     YPGQVDYGWLRSAEHKRANAKVREGEACLSCHEGEEAELGATLVKGGRHEPVPIAGKL
                     GAIALQVQAAHDDANLYLRFQWKTQMARAGQMHDYMMYDGEKWAFIGGPRSKEAVRSG
                     AQPPLYEDRLSVMIDDGKVPMFANQGCWLTCHTGMRDMPGEPTKEQVQAHPLIGQTHK
                     ESDVRKYLPATRTDEAASWDETRTPEEIARLKEAGAFVELMQWRGHRSNPVGMADDGY
                     VLDYRLVDAGKGPFSWNVDRKTMTPKFMFDPAKVGVKALALADVGNASKPHALIREDN
                     AVAYDPAAGWKKGDVLPGRLLSRADASGSAADNADVRGEWADGQWTVLWTRKLDTGHA
                     ADDKALKPGGVVNVGFAVHDDNVTTRFHHVSFPLTLGVGTKATISSVALE"
     gene            4806..5420
                     /gene="gmk"
                     /locus_tag="PKN25_13320"
     CDS             4806..5420
                     /gene="gmk"
                     /locus_tag="PKN25_13320"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNV91925.1"
                     /translation="MAGTLFIVTAPSGAGKTTLVRGLLQRDPRVQLSISYTTRAPRPG
                     EQDGREYNFVDVPTFRALRDRGEFLEWAEVHGNYYATSRVWLKQQVEAGRDTLLEIDW
                     QGAQQVRKHFPDAVGVFILPPSMDELEARLRGRNTDSDEVISRRLLGARGEMRHVAEF
                     DYVIINDEIDAALDDLVAVVRAARLRCAKQHQRHPEYFAFLEQD"
     gene            5423..5638
                     /gene="rpoZ"
                     /locus_tag="PKN25_13325"
     CDS             5423..5638
                     /gene="rpoZ"
                     /locus_tag="PKN25_13325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNV91926.1"
                     /translation="MARITVEDCLKRIPNRFQLTLAATYRARQLTAGGTPQIDVDPLD
                     RDKPTVIALREIEAGKVGLEMLNRGQA"
     gene            5656..>6709
                     /locus_tag="PKN25_13330"
     CDS             5656..>6709
                     /locus_tag="PKN25_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV91927.1"
                     /translation="MKAAEAGPAPEVFRPPVSSVLALDFQQLKTKLQTYLRPEDVGRV
                     EAAYHFSADAHAGQFRVSGEPYVSHPVAVCSLVADWHLDPQALIAALLHDVMEDTHIT
                     KAEIAERFGKVSAELVDGLSKLDKIEFRSQEEAQAENFRKMLLAMASDLRVILIKLAD
                     RLHNMRTLDHMRPAKRRRIANETLEIYAPIANRLGLNDLFRELQELSFRNKYPLRFEV
                     LAKAIRSARGNRREVVGKILASIEERLPQWGIAAEVQGREKHLYGIYRKMVEKHLSFS
                     QVLDIYGFRVIVKDVPSCYLALGALHSMYKPVPGKFKDYLAIPKANGYQSLHTTLIGP
                     FGMPVEVQIRTREMHHI"
BASE COUNT         1067 a         2262 c         2261 g         1019 t
ORIGIN      
        1 ctggcggtgc acagcacgcg ggtggcgccg aattcgacca gcaccgagcc ttcggcgtgg
       61 caggtgaagc cgcgggtgag gcggacggcg cggagttggt cgggctggcg caggctgggt
      121 cgcatcgtga ggtcctagga gcgggagtcg aatttgtgga tggcggcgtt gatctcgcgg
      181 atggcgcgct cgatctcgtc ctcgtcgagt tcggcgacgg ccgtcggacc cgggccggag
      241 aggtccaggc gggactcgaa gccgtccagt tccatggtgc gggtggagat cgattcgacg
      301 gtcgcggcgc cgacggcgtt ctcgtcgtgc cagcacatcg ccaccatcga caggttgtcg
      361 gcgctggcgc cggccttgcg ctcggcgacg tccatcagcc gcggcaccgc ccggctgagc
      421 acgccggcgg tgaggttcac caccaggccg tcgtcgtcga tcgggcccca cacgccgtcg
      481 gtgcagagcg cgagcaggtc gccgtcgcgc agcggggtgc gcggagaata ctccacctgc
      541 gggttgtagt tgccgccgag gcaggaatag atgcggttgc gcccggggtg gaggtgggcc
      601 tcctccggct tgatcacgcc ttggtcgatc atcagctggg tgcgcgaatg gtcgcgcgtg
      661 cgcgtgacca ggtggccgcg acgcagcagg tagagccgcg aatcgccggc gtgcgcccag
      721 tacgccgcgc cgcgctggat cacgcacgcg accagggtgg tgcgcggcgc ctccggcagc
      781 tggcggtcga gcgcatggtc gaggatggcg tggtgggcgc gttcgatcgc gcgcgcgagg
      841 aacagctggg ggtcgggcag cagcggccgc gcctctgcct ggaagcgctc ggcgatgcac
      901 tgcaccgcga tctgcgccgc cacctcgccg tgcaggtggc cgcccatgcc gtcggcgacc
      961 agcatcagca gggcgtcgcg cgagtagcag tgggcgatgc ggtcctggtt gtcgcgtcgt
     1021 ttgccggggc ggctttcctg gtagatggtg aagcgcatgg cgggtcctca gctgcggccg
     1081 atgaaggagc gcagccggct gcccaagtcc gagaaccagt gttccggacg ctcgggggcg
     1141 ttttcttgat ggatcagcgc cttctgcagc gcatacacgc tgggcgggcg ctgctgcggg
     1201 tcgaggtgaa ggcaccagtc gacgagctcg agcagctgcc ggctgtagcg cccggcctgg
     1261 gtcctcgcca ggggctggag cgtgtcttcc cgcgcgcgtt cgtccgcgcg cggcggcggg
     1321 ctgccgacga tgcaggcgtg caggctcgca cccacgctgt agatgtcggt ccacggaccg
     1381 agctgggcgc ccttctcgta ttgttccggc gcggcgtatc cgggcgtgta catcggcttg
     1441 agcaccggct ggtcgctcat cagggtctgg cgcgcggcgc cgaagtcgag cagcaccggg
     1501 gtgccgtcgt tgcgcaggta gatgttggac ggcttgatgt cgaggtgcat gagcttgtgc
     1561 gcgtgcacct cgcgcaggcc attgagcatg cgcgcgaaca ccgcgcggat cagcatctcc
     1621 ttgacctcgc cgcggtgctt gcggatgtag tcgtgcaggg tgcggccgcg ctcgaactgc
     1681 atgaccatgt acaccgtgcc gttggcgcgg aagaagttga gcacccgcac cacgttcgga
     1741 tgcatcagct tggcgagcga gcggccttcc tcgaagaagc acttcatgcc gtagcggaag
     1801 gcgaggcggt gctcctcgct gacctgcggt tcggtctcgc cttccttgcg cagggcgagc
     1861 gagttgggca ggtattcctt gatcgccacg gccatgccat gcgcgtcgcg cgcgagatag
     1921 acgatcgaga agccgcccag cgacagttgc cgctcgatgc ggtactggtc gagctgaaac
     1981 cccggtggca gcgcgcagtt ggcttgagac ggcatcttcg agagcgctag aatcgctgtt
     2041 ttcgcggttt cattcctgat ccaggcgccc agtttatccg atcgggcgcg gtccccggag
     2101 cgccccatgg tccagagcat gacaggtttc gcggttcaga cgcgagacct gggtagtgtc
     2161 agccttcatc tcgagctgcg cagcgtcaac tcgcgctatc tcgatctcgg cttccgcatc
     2221 gtcgacgacc tgcgcgccgc ggagcccgcg atccgcgagc gcatctccgc gcgcctcggc
     2281 cgcggcaagg tcgaatgtcg cctcaacctg caggccggcc acgccgcgcc gcgcagcatg
     2341 gcgctcaacg gcgcgctgct cgaccagctc gccgctgcgc aggacggctt gcgtgcgcgc
     2401 ttccccgacg ccgcgccgct gtcggtcaac gagctgctgc gctggccggg catgctcgcc
     2461 gacgacagcc tcggcttcga cgagatgctg ccggcgatcg tcgagctgac caacaccgcg
     2521 ctcgacgagc ttgtcgccac ccgccagcgc gagggcgaca agctcgccga catgatccgc
     2581 gagcgcgtcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccacccgcc
     2701 agcgcgaggg cgacaagctc gccgacatga tccgcgagcg cgtcgcacgc atgcgcgagc
     2761 tcgtcgccca ggcggccccg cgcatgccgg cggtggtgct cgagtaccag gagcgcctga
     2821 ccgccaagct gcgcgacgcc gtcgccagcc tggacgagga ccgcgtccgc caggaggtcg
     2881 cgctctacgc ccagcgcatc gacgtggacg aggagctcac ccgcctgtcc acccacctcg
     2941 acgaggtcga gcgcatcctg aagagcgccg gcgccgccgg caagcggctc gacttcctca
     3001 tgcaggagct caaccgcgag gccaacaccc tggcctccaa gtcgcccgcc accgacatca
     3061 ccgcgatcgc gatggagatg aaggtgctga tcgagcagat gcgcgagcag gtgcagaacc
     3121 tcgagtgagg aatgaccgcc cccgggagcg gggaggtgga agggccgggt gccggtctat
     3181 cgagcacgtg ccgttcgtgg taacaagagg gcctccaccc ggccgcttca ggttttttcc
     3241 ggctggcctg gagacccttg tgcgggcggg cacggcagcg ctgcaatcgc cagcgcccgg
     3301 cagcctccgc gcatgtttgt ccccggatca gacggagaga aatgatgact acatcgacat
     3361 ggagtgcttc cggcgccgca gcattcgccg cttgtgtggc tgctgcagcg acaccggcct
     3421 tcgccgcggc tcccgctgac tgggcagcca tcccggtgca gacggtgacg ctgttctatc
     3481 ccgggcaggt cgattacggc tggctgcgca gcgcggagca caagcgtgcc aatgccaagg
     3541 tcagggaggg cgaagcctgt ctgtcctgcc acgagggcga ggaggccgaa ctcggcgcca
     3601 ccctcgtcaa gggcgggcgg cacgaaccgg tcccgatcgc cggcaagctc ggtgcgatcg
     3661 cgctccaggt ccaggccgcc catgacgacg ccaatctgta cctccgcttc cagtggaaga
     3721 cgcagatggc gcgggccggg cagatgcacg actacatgat gtacgacggc gagaagtggg
     3781 ccttcatcgg cgggccgcgc tcgaaggaag ccgtgcgcag cggcgcccag ccgccgctct
     3841 acgaggatcg cctgtcggtg atgatcgacg acggcaaggt gccgatgttc gccaaccagg
     3901 gctgctggct gacctgccac accggcatgc gcgacatgcc cggagagccg accaaggagc
     3961 aggtccaggc ccatccgctg atcggccaga cgcacaagga aagcgacgtg cgcaagtatc
     4021 tgccggccac gcgcacggac gaggcggcga gctgggacga gacccgcacg ccggaggaga
     4081 tcgcccgcct caaggaggcg ggcgccttcg tcgagctgat gcagtggcgc ggtcaccgca
     4141 gcaatccggt gggcatggcc gacgatggct acgtgctcga ctatcgcctc gtcgacgccg
     4201 gcaagggccc gttcagctgg aacgtcgacc gcaagaccat gacgccgaag ttcatgttcg
     4261 accctgcgaa agtcggtgtg aaggcgcttg cgctcgcgga tgtcggcaac gcgtcgaagc
     4321 cgcacgcgct gatccgggaa gacaacgccg tggcctacga tccggccgcg ggctggaaga
     4381 agggcgacgt ccttcccggg cgcctgctct cacgcgccga cgcgagcggt tcggcggcgg
     4441 acaatgccga cgtccgcggc gagtgggcgg atggccagtg gacggtgctg tggacgcgca
     4501 agctcgacac cgggcatgcc gccgacgaca aggccctgaa gccgggcggc gtcgtcaacg
     4561 tgggctttgc cgttcatgac gacaacgtca caacgcgttt ccatcatgtg tccttcccgc
     4621 tgaccctggg ggtcggcacg aaagccacca tctcctcggt cgcgctcgag tgagcgcctt
     4681 ccggcaacgc ttgcggttgc agcacgctgc cgcccgcccg gcggctcccc tcggcggccg
     4741 ttctgctgcg tcggccgcct cccatccctc tgccggtgcg ctgcaccggc cctgagacaa
     4801 cgaccatggc cggaaccctt ttcatcgtca ccgcgccctc gggcgccggc aagaccaccc
     4861 tggtgcgcgg gctgctgcag cgcgacccgc gcgtgcagct gtcgatctcc tacaccacgc
     4921 gcgcgccgcg ccccggcgag caggacgggc gcgagtacaa cttcgtcgac gtgcccacct
     4981 tccgtgcctt gcgcgaccgc ggcgagttcc tcgaatgggc cgaggtgcac ggcaactact
     5041 acgccacctc gcgcgtgtgg ctcaagcagc aggtcgaggc cgggcgcgat accctgctcg
     5101 agatcgactg gcagggcgcg cagcaggtgc gcaagcactt cccggacgcg gtcggggtct
     5161 tcatcctgcc gccctcgatg gatgaactcg aggcccgcct gcgcggtcgc aataccgaca
     5221 gcgacgaggt catcagccgc aggttgctcg gcgcgcgcgg ggaaatgcgc catgtcgcgg
     5281 aattcgacta cgttataatc aacgacgaaa tcgatgccgc actcgacgac ctcgtcgccg
     5341 tggtgcgcgc cgcgcgtttg cgctgcgcca agcagcacca gcggcatccc gagtacttcg
     5401 cctttctcga acaggattga acatggcccg catcaccgta gaagactgcc tcaagaggat
     5461 cccgaaccgc ttccagctca ccctggcggc gacctatcgt gcccgccagc tcaccgctgg
     5521 cggcacgccg cagatcgacg tcgatccgct cgaccgggac aagccgaccg tgatcgcgct
     5581 gcgcgagatc gaagcgggca aggtcggcct cgagatgctc aaccgcggcc aggcctgaaa
     5641 tcgcccgggg gttgcatgaa agccgccgaa gcaggcccag cccccgaagt cttccgcccg
     5701 ccggtgtcga gcgtgctcgc gctcgacttc cagcagctca agaccaagct gcagacctac
     5761 ctccggcccg aggacgtggg ccgcgtcgag gcggcctacc acttctccgc cgatgcccac
     5821 gccggccagt tccgcgtcag cggcgagccc tatgtcagcc acccggtggc ggtgtgttcg
     5881 ctggtcgccg actggcacct cgacccgcag gcgctgatcg ccgcgctcct ccacgacgtg
     5941 atggaggaca cccacatcac aaaggcggag atcgccgagc gcttcggcaa ggtctccgcc
     6001 gaactcgtcg acggcctctc caagctcgac aagatcgagt tccgctcgca ggaagaggcg
     6061 caggcggaga acttccgcaa gatgctgctg gcgatggcga gcgacctgcg cgtcatcctc
     6121 atcaagctcg ccgaccgtct gcacaacatg cgcacgctgg accacatgcg tccggccaag
     6181 cgccgccgca tcgccaacga gacgctcgag atctacgcgc cgatcgccaa ccggctcggg
     6241 ctcaacgacc tcttccgcga gctgcaggag ctgtccttcc gcaacaagta cccgctgcgc
     6301 ttcgaggtgc tcgccaaggc gatccgctcg gcgcgcggca accgccgcga ggtggtcggc
     6361 aagatcctcg ccagcatcga ggagcgcctg ccgcagtggg gcatcgccgc cgaggtgcag
     6421 gggcgcgaga agcacctcta cggcatctac cgcaagatgg tcgagaagca cctgtccttc
     6481 tcgcaggtgc tcgacatcta cggcttccgc gtcatcgtca aggacgtgcc gagctgctac
     6541 ctcgcgctcg gtgcgctgca ctcgatgtac aagccggtgc cgggcaagtt caaggactat
     6601 ctggccatcc ccaaggccaa cggctaccag agcctgcaca ccaccctgat cggtcccttc
     6661 ggcatgccgg tcgaggtgca gatccgcacc cgcgagatgc accacatcg
//
LOCUS       DAOQBS010000148         6690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_11325_length_6690_cov_15.241899, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000148 DAOQBS010000000
VERSION     DAOQBS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11325_length_6690_cov_15.241899"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PKN25_13335"
     CDS             <1..782
                     /locus_tag="PKN25_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HNV91928.1"
                     /translation="LVFTLPSFPYVFKIIKDVFGASKNMDRATVKRKYLMVKHVDRVG
                     RMADTLEFSYAALPLARFHPELLEELRTLAPSSFEIDGDKVIIKHLYIERRMIPLNLH
                     LEKASEPELEEAVKEYGNAIRELAIANIFPGDMLWKNFGVTRYGRVVFYDYDEIEYMT
                     DMNFRKIPPAPYEEMEMSGEPWYSAGPMDVFPEEFATFLLGVPRVRKAFLKHHRDLLA
                     PKFWQEAQRRIREGYVEDFFPYPVELRFCKLFPPAAGAASS"
     gene            complement(813..1136)
                     /gene="soxZ"
                     /locus_tag="PKN25_13340"
     CDS             complement(813..1136)
                     /gene="soxZ"
                     /locus_tag="PKN25_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HNV91929.1"
                     /translation="MTNPMRIRADVRDGVADVRMQVPHPMESGQRKDDKGAIIPAHFI
                     TRLEVHHGERLVFGADFGPSVSANPFLRFRFKGAAAGDRLVARWVDSRGDSRSDEAVI
                     AGAPA"
     gene            complement(1164..1628)
                     /gene="soxY"
                     /locus_tag="PKN25_13345"
     CDS             complement(1164..1628)
                     /gene="soxY"
                     /locus_tag="PKN25_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HNV91930.1"
                     /translation="MDQKRRQLLHAVGGLGVLGLLATAGLISPAQAQAAAARPGFDAR
                     ELNAALAALGAQEASASAELSLAAPDIAEVANAVSVEVKSRLPGTDRIAILVEKNPFP
                     LAAVFSIPEGTQPEVITRLKMAQSADVVALVRANGRFHIARKAVIVTISGCG"
     gene            complement(1868..2308)
                     /locus_tag="PKN25_13350"
     CDS             complement(1868..2308)
                     /locus_tag="PKN25_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNV91931.1"
                     /translation="MHAGIPTGVHVLCRREGRVLLMRRAGTGFFDGLFSLPGGHVEPG
                     ESVRAAARRELREETGLEVEEGELAWLGVVHRRSDTNRIDFFLAAERFSGEPAILEPH
                     KCDRLEWHAPGALPERTVEYVRAALAAGPAPWILELGWKEAGTA"
     gene            complement(2308..2757)
                     /gene="dut"
                     /locus_tag="PKN25_13355"
     CDS             complement(2308..2757)
                     /gene="dut"
                     /locus_tag="PKN25_13355"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNV91932.1"
                     /translation="MHRIDVKLLDERLKTHPPAYATEGAAGLDLRACLPGPITLHPGE
                     TTLVPSGLAIHLSDPGLAAMVLPRSGLGHKHGIVLGNLIGLIDSDYQGQIFVSVWNRG
                     RASFTIEPMERIAQLVVVPVLQVGFNFVEGFEQSSRGADGFGSTGKH"
     gene            complement(2817..4013)
                     /gene="coaBC"
                     /locus_tag="PKN25_13360"
     CDS             complement(2817..4013)
                     /gene="coaBC"
                     /locus_tag="PKN25_13360"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNV91933.1"
                     /translation="MSMLSNRKIVLGVGGGIAAYKSAELVRLLGKAGAEVHVVLSEGG
                     ARFVTPVTYQALSGNPVWSDLWDARMPNHMAHIDLGRDADAILVAPATADFLARLAHG
                     HADDLLSTLCLARAVPLIVAPAMNRQMWESPPTQRNVAQIRADGVAVFGPAAGDQACG
                     EVGMGRMLEAEELLERLEGFFVPKLLAGRKVVLTAGPTFEALDPVRGITNISSGKMGY
                     ALARACAQAGAEVVLVSGPVSLPVPAEVRRVSVTSALEMRDAVLHALPGADVFIGVAA
                     VADYRPLASAEHKLKKSADTMNIALTPNPDILAEVAAHADAPFCVGFAAESRDLDAYA
                     EGKRRNKRLPMLVGNLVSDGMGGDDNTVILYDDAGRHPLPRAAKAEVARGIVAHLAGV
                     LPARGR"
     gene            4092..4769
                     /gene="radC"
                     /locus_tag="PKN25_13365"
     CDS             4092..4769
                     /gene="radC"
                     /locus_tag="PKN25_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNV91934.1"
                     /translation="MAITDWPADERPREKLLARGAAALSDAELLAIFLRVGIRGQSAV
                     DLARALLSRFGTLTRMCGADANEFAAVPGMGLAKYAQLQAVMELSRRALAETLVARPL
                     FDSPQVVRDWLRLRLGHLPHETFCVLLLDARHCLIEAVDLFRGTLTQTSVYPREVVKL
                     ALARNAAAVILAHNHPSGAAEPSTADELLTRALKDALGLVDVRVLDHFVVPAHGHPVS
                     FAERGLL"
     gene            4917..5153
                     /gene="rpmB"
                     /locus_tag="PKN25_13370"
     CDS             4917..5153
                     /gene="rpmB"
                     /locus_tag="PKN25_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNV91935.1"
                     /translation="MARVCQVTGKAPMVGNNVSHANNKTKRRFLPNLQSRRFWSEGEN
                     RWIRLRVSNAGLRTIDKKGIDVVVAELRARGEKL"
     gene            5179..5346
                     /gene="rpmG"
                     /locus_tag="PKN25_13375"
     CDS             5179..5346
                     /gene="rpmG"
                     /locus_tag="PKN25_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNV91936.1"
                     /translation="MAKGIREKIKLESTAGTGHFYTTSKNKRTTPGKLEFSKYDPVAR
                     KHVPYKEVKLK"
     gene            complement(5488..5691)
                     /locus_tag="PKN25_13380"
     CDS             complement(5488..5691)
                     /locus_tag="PKN25_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNV91937.1"
                     /translation="MATGTVKWFNDSKGFGFITPDDGSEDLFAHFSAITMSGFKSLKE
                     GEKVSFDVTQGPKGKQASNIQRA"
     gene            6080..6388
                     /gene="clpS"
                     /locus_tag="PKN25_13385"
     CDS             6080..6388
                     /gene="clpS"
                     /locus_tag="PKN25_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNV91938.1"
                     /translation="MGTRKQDDLVLEAKRTRTRPPPLFKVMLLNDDYTPMDFVIVVLQ
                     KYFGMDRERATRVMLQVHREGMGVCGVFPRDIATTKVEQVTSFARQHQHPLACVMEEN
                     "
     gene            6389..>6690
                     /locus_tag="PKN25_13390"
     CDS             6389..>6690
                     /locus_tag="PKN25_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNV91939.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALLDNPSAAQVL
                     KACAANIDELRRELTGFIEEHTPRIEGTDEIDTQPTLGFQRVIQRAILHVQSSGKK"
BASE COUNT         1112 a         2307 c         2189 g         1082 t
ORIGIN      
        1 tgctggtgtt cacgctgccc tccttcccct acgtgttcaa gatcatcaag gacgttttcg
       61 gcgcctcgaa gaacatggac cgcgccacgg tcaagcgcaa gtacctgatg gtcaagcacg
      121 tcgaccgcgt cgggcgcatg gcggacacgc tggagttctc ctacgcggcg ctgccgctgg
      181 cgcgcttcca ccccgaactg ctggaagagc tgcgcacgct cgcaccgtcc tcgttcgaga
      241 tcgacggtga caaggtgatc atcaagcatc tctacatcga gcgccgcatg atcccgctca
      301 acctccacct ggaaaaggcg agcgaaccgg agttggaaga ggcggtcaag gagtacggca
      361 acgccatccg cgagctcgcc atcgccaaca tcttccccgg cgacatgctg tggaagaact
      421 tcggcgtcac gcgctacggt cgcgtcgtgt tctacgacta cgacgagatc gagtacatga
      481 ccgacatgaa cttccgcaag atcccgcccg cgccctacga ggagatggag atgtcgggcg
      541 agccgtggta ctcggccggg ccgatggacg tcttcccgga ggagttcgcc accttcctgc
      601 tcggcgtgcc gcgggtgcgc aaggccttcc tcaagcacca ccgcgacctg ctcgcgccga
      661 agttctggca ggaagcgcag cggcgcatcc gcgagggcta cgtcgaggac ttcttcccct
      721 acccggtcga gctgcgcttc tgcaagctct ttccgccggc cgcgggcgca gcttcaagct
      781 gagcgcccgc ccgggagccg gccggcaagc gctcaggccg gcgcgccggc gatcaccgcc
      841 tcgtcgctgc ggctgtcgcc gcggctatcg acccagcgcg ccaccaggcg atcgcccgcg
      901 gccgccccct tgaaacggaa gcgcaggaag gggttggccg aaacggatgg gccgaagtcg
      961 gcgccgaaga cgaggcgttc gccgtggtgg acctccagcc gggtgatgaa gtgcgcgggg
     1021 atgatcgcgc ccttgtcgtc cttgcgctgg ccgctctcca tcggatgagg gacctgcatg
     1081 cggacgtcgg cgacgccgtc gcggacgtcc gcgcggatgc gcatcgggtt tgtcatggag
     1141 tcgctctcct gtgccggcgc ggctcagccg cagccgctga tggtgacgat gaccgccttg
     1201 cgggcgatgt ggaagcggcc gtttgcccgc accagcgcga ccacgtcggc actctgcgcc
     1261 attttcagcc gggtgatcac ctcgggctgg gtgccctccg ggatggagaa caccgcggca
     1321 agcgggaagg gatttttctc gaccaggatc gcgatgcggt cggtgccggg caggcggctc
     1381 ttcacctcga ccgagacggc gttggcgacc tcggcgatgt cgggggcggc gagactcagc
     1441 tcggcgctcg cactggcctc ctgcgccccc agcgcggcga gcgccgcgtt cagctcgcgg
     1501 gcgtcgaaac cgggccgcgc ggcggcggcc tgcgcctgcg cgggcgagat cagaccggcg
     1561 gtggcaagca ggcccagcac gcccaagccc ccgacggcat gcagcaattg tcttcgtttt
     1621 tggtccatcc ggctctcctg cagggttcgc atggcggcag cgcgtggccg ccgcgtgcgg
     1681 caatactcgc acacccggga ccgctctggc gcagcgcagc atcgtccatc cggcgagcac
     1741 gccgtcgcgc gtcgagcgcg gcttggcggg cttccggtcg aaatcgacgc cgagcgcgag
     1801 cacgtcctcc ggcgcgagac ccgccgccac actcgccatg gcgtcgctcc tgcgcacagc
     1861 gtggcggcta cgcggtgccc gcctccttcc aaccgagctc caggatccac ggcgccgggc
     1921 cggccgcgag cgcggcgcgc acgtactcga ccgtacgctc gggcagggcc ccgggcgcgt
     1981 gccattcgag gcgatcgcac ttgtgcggct cgaggatggc gggctcgcca ctgaagcgct
     2041 ccgcggcgag gaagaagtcg atgcgattgg tgtccgagcg ccgatggacg acacccagcc
     2101 aggccagctc gccctcctcg acctccagcc cggtctcctc gcgcagctcg cgccgcgccg
     2161 ccgcgcgcac cgactcgccc ggctccacgt gtcccccggg caggctgaag aggccgtcga
     2221 agaaacccgt tccggcacgg cgcatcagca gcacgcggcc ctcgcgccgg cacagcacat
     2281 ggacgccggt aggaatccct gcgtgcatca gtgcttgccc gtgctgccga agccgtccgc
     2341 cccgcgcgag ctctgctcga agccctcgac gaagttgaag ccgacctgca gcaccggcac
     2401 caccacgagc tgggcgatgc gctccatcgg ctcgatggtg aagctcgcgc gcccacggtt
     2461 ccacaccgag acgaagatct gcccctggta gtcggagtcg atcagcccga ttaggttgcc
     2521 gagaacgatg ccgtgcttgt ggccgagccc ggagcgtggc agcaccatcg ccgccagccc
     2581 cgggtcggaa agatggatcg cgaggccgct cggcaccagc gtggtctcgc ccgggtgcag
     2641 ggtgatcggg ccgggcagac aggcgcgcag gtcgagcccc gcggcgccct ccgtggcgta
     2701 ggcgggggga tgggtcttga ggcgctcgtc gagcagcttg acgtcgattc ggtgcatgtc
     2761 ggctctcctt gccggcgggc ggcgcgtgcc ggcccagcct gtcgttgcgt ttgcgctcag
     2821 cgcccgcggg ccggcagcac gccggcgagg tgggcgacga tgccgcgcgc cacctcggcc
     2881 ttggccgcac gcggcagcgg gtggcggccg gcgtcgtcgt agaggatcac ggtgttgtcg
     2941 tcgccgccca tgccgtcgct gaccaggttg ccgaccagca tcggcagccg cttgttgcgt
     3001 cgcttgccct cggcgtaggc gtcgaggtcg cggctctcgg cggcgaaacc gacgcagaaa
     3061 ggcgcgtcgg catgtgcggc cacctcggca aggatgtccg ggttgggggt cagcgcgatg
     3121 ttcatggtat cggcggactt tttcagcttg tgttcggcac tcgccagcgg ccggtaatcg
     3181 gccaccgcgg ccacgccgat gaagacgtcc gcacccggca gcgcgtggag caccgcgtca
     3241 cgcatctcca gcgcgctggt cacgctcacg cgcctcacct cggccggcac cggcaggctc
     3301 accggtccgc tcaccagcac cacctccgcg cccgcctggg cgcaggcgcg cgcgagcgcg
     3361 tagcccatct tgcccgagct gatgttggtg atgccgcgca ccggatcgag cgcctcgaag
     3421 gtcggcccgg cggtgagcac gaccttgcgt ccggcgagca gcttgggcac gaagaagccc
     3481 tcgaggcgtt cgagcagctc ctccgcctcg agcatgcgcc ccatgccgac ctcgccgcag
     3541 gcctggtcgc ccgccgcggg accgaacacg gcgacgccgt cggcgcggat ctgcgcgacg
     3601 ttgcgctgcg tcggcggcga ttcccacatc tggcggttca tcgccggggc gacgatcagc
     3661 ggcacggcgc gcgccaggca aagcgtgctg agcaggtcgt cggcgtggcc atgagcgagg
     3721 cgggcaagga aatctgcggt cgccggggcg acgaggatgg catcggcgtc gcggccgaga
     3781 tcgatgtgcg ccatgtggtt gggcatgcgc gcatcccaca ggtcggacca caccggattg
     3841 cccgacagcg cctggtaggt caccggggtc acgaagcgcg ccccgccctc gctcagcacc
     3901 acgtgtacct cggcgccggc cttgccgagc aggcgcacca gctcggccga cttgtacgcg
     3961 gcgatgccac cgccaacccc gaggacgatc ttgcgattgc tcaacatgct catgacgatg
     4021 aactatactc cgcgatggat cgatgcattc tggataggcc gacaggccac gacgagggag
     4081 gcggacacgc gatggcgatc accgactggc ctgcggacga acgtccgcgc gagaagctcc
     4141 tcgcccgcgg cgccgcggcg ctgtccgacg cggagctgct ggcgatcttc ctgcgcgtgg
     4201 gcatccgcgg gcagagcgcg gtcgacctcg cccgcgcgct gctgtcccgc ttcggcaccc
     4261 tgacccgcat gtgcggcgcc gacgcgaacg agttcgccgc ggtacctggc atggggctgg
     4321 cgaagtatgc ccaactgcag gcggtcatgg aactgtcgcg gcgggcgctc gccgagacgc
     4381 tggttgcacg cccgctgttc gattcacccc aggtcgtacg cgactggctg cgcctgcgcc
     4441 tcggacacct gccccacgag accttctgcg tgctgctgct cgacgcacgc cactgcctga
     4501 tcgaggcggt ggatctcttc cgcggcacgc tcacgcagac ctcggtgtat cctcgcgagg
     4561 tggtcaagct cgccctcgcg cgcaacgccg cggccgtgat cctcgcccac aaccatccct
     4621 ccggtgccgc cgagccgagc accgccgacg agctgctcac gcgtgcgctg aaggacgctc
     4681 tcggcctggt cgacgtgcgc gtgctcgatc atttcgtggt gccggcgcac ggtcatccgg
     4741 tatcattcgc cgaacgcggc ctgctttgag gttccgcccg cccttccgct ccggccccgg
     4801 accctgcctg gcattccacc ctgcgaacac gacgaaggta cgcggggctt gcctcgaccg
     4861 cgcttcttgc attatcctcg cacgttttcc gcaatcagaa ttccctggag catcgtatgg
     4921 ctcgcgtctg ccaagtgacc ggtaaagcac cgatggtggg aaataacgtt tcccacgcca
     4981 acaacaagac caagcgtcgt ttcctgccca acctgcagag ccgccgcttc tggagcgaag
     5041 gcgagaaccg ctggatccgc ctgcgcgtgt ccaacgcggg tctgcgcacg atcgacaaga
     5101 aaggcatcga cgtcgtcgtt gccgagcttc gcgcccgtgg cgagaagctg taagcaaccg
     5161 aactgaacgg agaaggccat ggccaagggc atccgcgaaa agatcaaact ggagtcgacc
     5221 gccggtacgg gtcacttcta caccacctcg aagaacaagc gcaccacacc cgggaagctc
     5281 gagttcagca agtacgaccc ggtcgcgcgc aagcacgtgc cgtacaagga agtgaagctg
     5341 aagtgatttg gcgcggcggt tgcacgcaac cgcccggcag aacgcggcag ctccagaaaa
     5401 ccggaagggc catcggaaga tccgatggcc cttctgtttt ttcgcccgcc accggcagtg
     5461 gcgggcacga tcacatgcgc cgcggaatca cgcgcgctgg atgttcgagg cctgtttgcc
     5521 cttgggaccc tgggtgacgt cgaaggagac cttctcgcct tccttcaggc tcttgaaacc
     5581 gctcatcgtg atggcggaga aatgtgcgaa caggtcttca cttccatcat ccggcgtgat
     5641 gaaaccgaaa cccttggaat cgttgaacca cttcacggta ccagttgcca tattgcttgg
     5701 atccttcaag gttgcttgtt acaccgcggc cttcgcaaac cgcggtcgtg tgggcgctga
     5761 cagggaccaa aaccacctcc ggaagcccct cgggatcagg ctcgagccgg cgctcgcctt
     5821 cttttttacg cattcggcac ggcagcgtca acgggatttc gcggggtttc gatcgcgcag
     5881 cgcaacatga atgcgcacgg acccaccccc atcccgagcg cgatttcgcg cctggcgcgg
     5941 agatgcgttc atgccgcagc gcaagaagcc tgtttcgggc agtgcgcgcc aaaccacact
     6001 ctgcatgggg ttttcccggg actcgacgcg cacacgaagc gcgtgttgca ctctgcttcg
     6061 acttcattag aatgatttta tgggcacccg aaaacaagac gatctggtcc tggaagccaa
     6121 gcggacgcgc acgcgcccgc ccccgctctt caaggtcatg ctgttgaacg acgactacac
     6181 gccgatggat ttcgtcatcg tcgtcctgca gaaatatttc ggcatggatc gcgaacgtgc
     6241 cacgcgagtc atgctccaag tccacagaga gggcatgggg gtatgcggcg tctttcccag
     6301 ggacatcgcc acgaccaagg tggagcaggt cacctcgttt gctcgtcagc atcaacatcc
     6361 gctggcatgc gtgatggagg aaaattgaat gatcgcgcaa gagcttgaag tcagcctgca
     6421 catggctttc gtcgaggcac ggcagaagcg ccacgagttc atcaccgtgg agcatcttct
     6481 gcttgccctg ctggacaacc cttccgccgc ccaggtgctg aaggcgtgtg ccgccaacat
     6541 cgacgagttg cgtcgcgaac tgaccggttt catcgaagaa cacaccccgc gcatcgaagg
     6601 cacggacgag atcgacaccc agcccacgct gggcttccag cgcgtgatcc agcgcgcgat
     6661 cctgcacgtg cagtcctcgg gcaagaagga
//
LOCUS       DAOQBS010000149         6623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_11491_length_6623_cov_13.973965, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000149 DAOQBS010000000
VERSION     DAOQBS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11491_length_6623_cov_13.973965"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..863)
                     /gene="fliR"
                     /locus_tag="PKN25_13395"
     CDS             complement(66..863)
                     /gene="fliR"
                     /locus_tag="PKN25_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNV91940.1"
                     /translation="MLSVTAAQLDAWLAALMFPLGRLLGLFASAPVFSNRGVSVRIRL
                     AIGLGVAMAVLPVLPPMPQVPPGGGLGLAIFAQQILIGIAIGFVMRIVFAAVDMAGAL
                     IGMQMGLSFAVFFDPDAGGQTAVLSDFLNLLATLIFLAIDGHLLMIDLLVRSFEWLPV
                     ATDAVDAGGWAFLARSGITVFSAGLLLSLPVIAVLLVANIALGVLTRAAPQLNLFAVG
                     FPITLTAGFIGVLLIMSNFAPVLQSLFERSYDTIGLLLDALAPRPTP"
     gene            complement(898..1167)
                     /gene="fliQ"
                     /locus_tag="PKN25_13400"
     CDS             complement(898..1167)
                     /gene="fliQ"
                     /locus_tag="PKN25_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNV91941.1"
                     /translation="MTPTTVIELGRQAIEVTLMVSAPLFLAALVTGLIISIFQAATQI
                     NEMTLSFVPKLVAIFVTLVLAGPWMITLLTDFIRRLFESIPAMIG"
     gene            complement(1193..1966)
                     /gene="fliP"
                     /locus_tag="PKN25_13405"
     CDS             complement(1193..1966)
                     /gene="fliP"
                     /locus_tag="PKN25_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360997.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNV91942.1"
                     /translation="MKPERRAPSHPVALLRLALACALAGLPLLATAQALPALTTSPAA
                     GGGTTYSLTIQTLMLMTLLSFIPALVLMTTCFTRIVIVFSLLRQAMGTQTAPPNQVLL
                     GLALFLTFFVMAPVGERVYTEAYLPMSEGRIQFDEALERASVPVKGFMLKQVREPDLA
                     LFAGLAKLPPVDKAEDLPMRVVIPAFVTSELKTAFQIGFIVFIPFIIIDMVVASVLMS
                     MGMMMMSPVIVSLPFKIMLFVLVDGWTLLIGSLVQSFAV"
     gene            complement(1956..2318)
                     /gene="fliO"
                     /locus_tag="PKN25_13410"
     CDS             complement(1956..2318)
                     /gene="fliO"
                     /locus_tag="PKN25_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNV91943.1"
                     /translation="MPELGASLVQMSFGLAVVVGLLFACLWLLRRLSAPRGGGAAIKV
                     LGAAAVGPRERVVLVEIGEDVLVLGVAPGSVARLHELKRADLPLPADLPAQPLPGGKT
                     FATWLRQAAQRRERRDEA"
     gene            complement(2327..2794)
                     /gene="fliN"
                     /locus_tag="PKN25_13415"
     CDS             complement(2327..2794)
                     /gene="fliN"
                     /locus_tag="PKN25_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNV91944.1"
                     /translation="MADENLITDDDWAAAMQEQAAAEEGGDSEADRVAAELAAAAMGD
                     SPYQARPASQLFEDFGAPAGKPGALNDFDMILDIPVQITVELGRTKLSIRNLLQLAHG
                     SVVELDGLAGEPMDVLVNGTLIAQGEVVVVNDKFGIRLTDIITPAERMRKIRN"
     gene            complement(2787..3797)
                     /gene="fliM"
                     /locus_tag="PKN25_13420"
     CDS             complement(2787..3797)
                     /gene="fliM"
                     /locus_tag="PKN25_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNV91945.1"
                     /translation="MSADFLSQEEVDALLRGVTGESDEPVADEAGVEGVRPYNLATQE
                     RIVRGRMPTLELINERFARYLRIGLFNYMHRNAEVSAGPIKVQKYAEFVRNLVVPTNL
                     NLITAKPLRGTGLVVFDPNLVFLVVDNMFGGDGRFHTRVEGRDFTPTEQRIIRGMLDV
                     VFAEYVRAWAPVFNLQPEFIRSEMNSQFANIATPSEVVVSTSLSVEFGGSQAEMHICF
                     PYSMLEPIRDLLYSSMQSDHLTSDRRWIKLLTRQLQTAEVELACSFGTARVSLRDIVD
                     MRVGDVIPLAVQPVLQAEVDGVPVFECRQGTRNGRYALRVERFITAEDNEPPMIPGAQ
                     NG"
     gene            complement(3810..4382)
                     /locus_tag="PKN25_13425"
     CDS             complement(3810..4382)
                     /locus_tag="PKN25_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNV91946.1"
                     /translation="MAKAPAKTEEGDETPKKKGKLGLILIIVLVVIVLVLGALVALLL
                     LKGGKDKEHEEPAAHAAPAAQQLHTTGTVDLTKPPTFIPLDAFTVNLRRDEGDHYLQV
                     VAVMRVADATMDPTLKAFMPEIRHRINLLLSSKLPSEVATVEGREALSLAITESVNEA
                     LGFKPVRDAAGKPVPNGPVQAVLFTSFIIQ"
     gene            complement(4476..5912)
                     /locus_tag="PKN25_13430"
     CDS             complement(4476..5912)
                     /locus_tag="PKN25_13430"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNV91947.1"
                     /translation="MSAPFSPSSLGNVAQLRLGAQQARIGISALDGLGAQPDSFSRTL
                     ENRMNTPEPAPRKPESPPPAPRPPERTASAEPAREPERPAGSGPAAGDRSAGPATQAD
                     AGKAAASGTSAPDKGATDARTGGAGAAAGEDAATAQADAAGEAADAAASPQAVASQAG
                     HAARLAALAAGAAVDADATSADTVTATASSPTDPAALAGLPAAIAALLAGRAEAPSGA
                     DATGSEAGTLQLVADGGRKSLQNSALLPKTDLSATAGGNTGPDGNATPAFLLQGREFA
                     AATMAARGASGAGAGGTAADGLEVPGTSNANGNLPAGLAHTHGATHLLRQASSAHAAS
                     PQLPVATPAGEQGWAEDVGNQVRWMLGRAESKAELVLTPPNMGKLEVSINLAGDQTTA
                     QFIASSQAARDALERALPQLREALQQAGILLGDANVSTSGQGREGDGREGSGGRGERG
                     EAGASGHAGTGGAGVWLKQQQGMVDTFA"
     gene            complement(6032..6472)
                     /gene="fliJ"
                     /locus_tag="PKN25_13435"
     CDS             complement(6032..6472)
                     /gene="fliJ"
                     /locus_tag="PKN25_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNV91948.1"
                     /translation="MPASFHLQPLLDLASTRTDEAARRLGELVAAERDVEQKLKMLED
                     YRTEYHERFVQAARDGLSADAWRNYGAFIAKLDDAIAAQRKVVEQSRARTVEGQQAWL
                     AQRTKLKAFDTLAQRHQHAVARSEARQEQKQSDEHAARRGGEDA"
BASE COUNT          978 a         2200 c         2451 g          994 t
ORIGIN      
        1 gtcgccagcg gcgagatccg cctggtgcgt tcgcgcacct tcccggagtt ctgagcgcgg
       61 ccctctcagg gtgtggggcg tggcgcgagc gcgtcgagca gcaggccgat ggtgtcgtag
      121 ctgcgctcga agaggctttg cagcaccggc gcgaagttgc tcatgatcag cagcacgccg
      181 atgaagcccg cggtcagcgt gatcggaaag cccaccgcga agaggttgag ctgcggcgcc
      241 gcgcgggtca gcacgccgag cgcgatgttg gccaccagca agaccgcgat caccggcagc
      301 gacagcagca gccccgccga gaacaccgtg atgccgctgc gcgcgaggaa ggcccagccg
      361 ccggcatcca ccgcatcggt cgccaccggc agccactcga agctgcgcac caggaggtcg
      421 atcatcagca ggtggccgtc gatggcgagg aagatcaggg tcgcgagcag gttgaggaag
      481 tcggacagca ccgcggtctg cccgcccgcg tcggggtcga agaagaccgc gaacgagagg
      541 cccatctgca tcccgatcag ggcgccggcc atgtccaccg cagcgaatac gatgcgcatc
      601 acgaagccga tggcgatgcc gatcaggatc tgctgcgcga agatcgccag gcccagtccg
      661 ccgccgggcg gcacctgcgg catcggcggc aggaccggca gcaccgccat cgccacgccc
      721 agcccgatcg ccaggcggat gcgcaccgac acgccacggt tggagaacac cggcgccgac
      781 gcgaacagcc cgagcaggcg cccgagcggg aacatcagcg ccgccagcca ggcgtcgagc
      841 tgggctgcgg tgacggagag catggtggcg gggaagggcg aggggcgggg aggaggctca
      901 gccgatcatc gccgggatcg actcgaacag ccggcggatg aagtcggtga gcagggtgat
      961 catccacggc ccggccagca ccagggtcac gaagatcgcc accagcttgg gcacgaagga
     1021 gagcgtcatc tcgttgatct gggtggcggc ctgaaagatg ctgatgatca ggccggtgac
     1081 gagcgcggcg aggaagagcg gcgccgacac catcagggtg acctcgatgg cctggcggcc
     1141 gagctcgatg acggtggtgg gggtcatggc gtttgtcctc gggcgggcgc ggtcacaccg
     1201 cgaagctctg caccagcgag ccgatcagca gcgtccagcc gtcgaccagc acgaacagca
     1261 tgatcttgaa aggcagcgac acgatcaccg gcgacatcat catcatgccc atggacatca
     1321 gcaccgaggc cacgaccatg tcgatgatga tgaaggggat gaagacgatg aagccgatct
     1381 ggaaggcggt cttgagctcg gaggtgacga aggcggggat caccacccgc atcggcaggt
     1441 cctcggcctt gtccacgggc ggcagcttgg ccagcccggc gaagagcgcg aggtcgggct
     1501 cgcgcacctg cttgagcatg aagcccttga ccggcaccga ggcgcgctcg agcgcctcgt
     1561 cgaactggat gcggccttcg gacatgggca gataggcctc cgtgtatacg cgctcgccga
     1621 ccggcgccat cacgaagaag gtgaggaaga gggcgaggcc cagcagcacc tggttgggcg
     1681 gcgcggtctg cgtgcccatg gcctggcgca gcagcgagaa cacgatgacg atgcgggtga
     1741 agcaggttgt catcagcacc aatgccggga tgaagctcag gagcgtcatc agcatcagcg
     1801 tctggatggt caggctgtag gtcgtgccgc cgcccgccgc cgggctggtg gtgagcgcgg
     1861 gcagggcctg cgccgtcgcc agcaggggca ggccggcgag cgcgcaggcg agcgcgaggc
     1921 gcagcagtgc cacggggtgg ctcggggcgc ggcgctcagg cttcatcgcg gcgttccctg
     1981 cgttgcgcag cctggcgcag ccaggtggcg aaggtcttgc cgcccggcag gggctgggcc
     2041 ggcaggtcgg cgggcagcgg caggtcggcg cgcttgagtt cgtgcaggcg cgcgacgctg
     2101 ccgggcgcca cgcccagcac cagcacgtct tcaccgatct cgaccagcac cacgcgctcg
     2161 cgcgggccga ccgcggccgc gccgagcacc ttgatcgccg cgccgccgcc gcgcggggcg
     2221 gacaggcggc ggagcagcca caggcaggcg aacagcaggc cgaccaccac cgccaggccg
     2281 aagctcatct gcacgagact ggcgccgagt tcgggcatcg cgctgctcag ttgcggatct
     2341 tccgcatgcg ctcggcgggg gtgatgatgt cggtgaggcg gatgccgaac ttgtcgttga
     2401 ccaccacgac ctcgccctgg gcgatcaggg tgccgttcac cagcacatcc atgggctcgc
     2461 cggccaggcc gtcgagctcg accaccgagc cgtgcgcgag ctgcagcagg ttacggatcg
     2521 acagcttggt gcgcccgagc tccacggtga tctgcaccgg gatgtcgagg atcatgtcga
     2581 agtcgttgag cgcgcccggc ttgcccgccg gggcgccgaa gtcctcgaag agctggctgg
     2641 cggggcgggc ctggtagggc gagtcgccca tcgccgcggc cgcgagctcg gcggcgaccc
     2701 gatcggcttc gctgtcgccg ccttcctcgg cggcggcctg ttcctgcatc gcggctgccc
     2761 agtcgtcgtc ggtaatcagg ttctcgtcag ccattctggg ctcccggaat catggggggt
     2821 tcgttgtcct cggcggtgat gaagcgctcc acgcgcagcg catagcgccc gttgcgcgtg
     2881 ccctggcggc attcgaacac cggcacgccg tcgacctcgg cctgcagcac cggctgcacc
     2941 gcgagcggga tcacgtcacc gacgcgcatg tcgacgatgt cgcgcaggct cacccgcgcc
     3001 gtgccgaacg agcatgcgag ttccacctcg gcggtctgca actgacgtgt cagcagcttg
     3061 atccagcgcc ggtccgaggt gaggtggtcg ctctgcatcg aggaatagag caggtcgcgg
     3121 atcggctcca gcatcgagta cgggaagcag atgtgcatct ccgcctgcga gccgccgaac
     3181 tcgaccgaga ggctggtcga caccaccacc tcggagggcg tcgcgatgtt ggcgaactgc
     3241 gagttcatct ccgagcggat gaactcgggc tgcagattga acaccggtgc ccaggcgcgc
     3301 acgtattcgg caaacacgac gtcgagcatg ccgcggatga tgcgctgctc ggtcggggtg
     3361 aaatcgcggc cctccacgcg ggtgtggaag cgcccgtcgc cgccgaacat gttgtccacc
     3421 accaggaaga ccaggttggg gtcgaacacg accagcccgg tgccgcgcag cggcttggcg
     3481 gtgatcaggt tgagattggt cggcaccacc aggttgcgca cgaactcggc gtacttctgc
     3541 accttgatcg gccccgccga cacctcggcg ttgcggtgca tgtagttgaa caggccgatg
     3601 cgcaggtagc gcgcgaagcg ctcgttgatg agctcgagcg tgggcatgcg cccgcgcacg
     3661 atgcgttcct gggtggcgag gttgtagggg cgtacgccct cgacgccggc ttcgtccgcg
     3721 accggctcgt ccgactcgcc ggtgacgccc cgcagcaggg cgtcgacctc ttcctgggag
     3781 agaaagtccg cggacatggc ggcgatccct tactggatga tgaaggaggt gaacagcacg
     3841 gcctgcaccg ggccgttggg caccggcttg ccggcggcgt cgcgcaccgg cttgaagccg
     3901 agcgcctcgt tcacgctctc ggtgatcgcc agcgacaagg cctcgcggcc ctcgacggtg
     3961 gccacctcgg acggcagctt gctcgacagc agcaggttga tgcggtggcg gatctccggc
     4021 atgaaggcct tcagcgtcgg gtccatggtc gcgtcggcca cgcgcatcac ggcgaccacc
     4081 tgcaggtagt ggtcgccctc gtcgcggcgc aggttgaccg tgaaggcgtc gagcgggatg
     4141 aaggtgggcg gcttggtgag gtccaccgtg ccggtggtgt gcagctgctg cgcagccggc
     4201 gcggcgtggg ccgccggctc ctcgtgctcc ttgtccttgc cgcccttgag cagcagcagc
     4261 gcgacgagcg cgccgagcac gagcacgatc accacgagca cgatgatgag gatcaggccg
     4321 agcttgccct tcttcttggg ggtctcgtcg ccttcctcgg tctttgccgg cgccttggcc
     4381 atgcgaatct cctgtagtac ggattgaatt atccgggagg tccgcacccg ccaaagcgcc
     4441 gattagtggg gcaagcccgc cccaattcgc gcgcttcagg cgaaggtgtc gaccatcccc
     4501 tgttgctgtt tcagccacac cccggcaccg ccggtgccgg cgtggccact cgcgcccgcc
     4561 tcgccgcgct cgccacgacc gccgctgcct tcgcgtccat cgccctcgcg gccctggccg
     4621 gaggtgctca cgttggcgtc gcccagcagg atgccggcct gctgcagcgc ctcgcgcagc
     4681 tgcggcagcg cgcgttcgag cgcgtcgcgc gcggcctggc tcgaggcgat gaactgcgcc
     4741 gtcgtctggt cgccggcgag gttgatcgac acctcgagct tgcccatgtt cggcggcgtc
     4801 agcacgagtt cggccttgct ctcggcacgg ccgagcatcc agcgcacctg attgccgacg
     4861 tcttccgccc agccctgctc gccggccggg gtcgccaccg gcagctgcgg cgatgcggcg
     4921 tgcgcggagg acgcctggcg cagcagatgt gtcgcgccat gcgtgtgcgc caggccggcc
     4981 ggcaggttgc cgttcgcgtt gctcgtgccc ggcacctcga ggccatccgc cgcggtgccg
     5041 cctgcaccgg cgccgctcgc gccgcgagcc gccatggttg cggcggcgaa ctcgcggccc
     5101 tggagcagga aggccggcgt ggcgttgcca tcgggaccgg tgttgccgcc agccgtggcc
     5161 gacagatcgg ttttcggcag cagtgcggaa ttctgaagcg acttgcgccc gccgtccgca
     5221 accagctgca gcgtgcccgc ttcggagccg gtcgcatccg cgccggaggg ggcttcggcg
     5281 cgaccggcga gcagtgcggc aatcgctgcc ggcaggccgg caagggctgc cgggtcggtg
     5341 ggcgagcttg ccgtcgcggt gaccgtatcg gcagatgttg cgtcggcgtc gaccgcggcg
     5401 cccgcggcca gggcggcgag gcgcgcggcg tggcccgcct ggctcgcgac ggcctgcggc
     5461 gaagcggcgg catcggctgc ctcgccggcg gcgtccgcct gcgcggtggc ggcgtcctcg
     5521 ccggcggcgg cgcccgcccc gccggtgcga gcgtccgtag cgcccttgtc aggtgcggac
     5581 gtaccggaag cggcggcctt gcctgcgtcc gcctgtgtgg cgggcccggc gctgcggtca
     5641 cccgcggcgg ggccgctgcc ggcagggcgc tcgggctcgc gtgcgggttc ggcggaggcc
     5701 gtgcgctcgg gcggtcgcgg ggccgggggg ggcgactcgg gcttgcgcgg cgccggctcg
     5761 ggcgtgttca tgcggttctc gagcgtgcgc gagaagctgt cgggctgcgc gcccaggccg
     5821 tccagggcgg agatgccgat gcgggcctgc tgcgcgccga ggcgaagctg cgcgacgttg
     5881 ccgagactgg agggggagaa cggtgcggac atggttgcgg gcctctcgtg ggctggatgc
     5941 gatggatcgc ggacgatccg acagcccatc aaaagcaagc ctcatgccat gggtgtgtca
     6001 ccgggtcggg cggctgccgc ggtttcccgc gtcaggcgtc ctcgccaccg cgtcgggcgg
     6061 cgtgctcgtc cgactgcttc tgctcctggc gcgcctcgct gcgcgcgacc gcgtgctggt
     6121 ggcgctgggc gagggtgtcg aaggccttga gcttggtgcg ctgggcgagc caggcctgct
     6181 ggccctcgac ggtgcgcgcg cgcgattgct cgaccacctt gcgctgcgcg gcgatcgcgt
     6241 cgtcgagctt ggcgatgaag gcgccatagt tgcgccatgc gtccgcgctc aggccgtcgc
     6301 gcgcggcctg cacgaagcgc tcgtgatatt ccgtgcgata gtcctcgagc atcttcagct
     6361 tctgttccac gtcgcgttcg gcggcgacga gttcgcccag gcgacgcgca gcctcgtcgg
     6421 tgcgggtgct ggcgaggtcg agcaggggct ggaggtggaa ggaggcgggc atcggcgtcg
     6481 tgtcacgggg gcgttgcaaa cagcattcta gccgttcgcc ggatgcccga ggtgggcata
     6541 tttcggtcgg ctcgtcctcg accgtgacga aatgctcgcg atcgatgtcg cgggtgcagg
     6601 agcgacgcca gtcgcgatcg cgg
//
LOCUS       DAOQBS010000150         6446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_11969_length_6446_cov_16.717728, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000150 DAOQBS010000000
VERSION     DAOQBS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11969_length_6446_cov_16.717728"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKN25_13440"
     CDS             complement(<1..365)
                     /locus_tag="PKN25_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNV91949.1"
                     /translation="MLRIITLNLNGIRSACTKGLLDWLPAQQADFLCVQELKAQDADL
                     RPEMRAPAGMQGWFHAAEKKGYSGVGIYSRHAPDRVVEGLGIPAIDAEGRFLQLDFGK
                     LSVVSLYLPSGSSSEERQQV"
     gene            405..1049
                     /gene="pyrE"
                     /locus_tag="PKN25_13445"
     CDS             405..1049
                     /gene="pyrE"
                     /locus_tag="PKN25_13445"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNV91950.1"
                     /translation="MDFSRDFIALACRKGVLRFGAFVTKAGRNSPYFFNAGLFDDGAS
                     FRELCGYYARTIRAAGIACDMLFGPAYKGIPLVAGTAIRLAEDGVDLPFAFNRKEAKD
                     HGEGGTLIGAPLRGKVLILDDVISAGTSVRESVELIRAAGAEPAGVVIALDRMERGKS
                     ALSAVEEVKENFGIPVVAVATLEDLVAYLADSPELAANLDAVQAYREQYGISSR"
     gene            1030..1704
                     /locus_tag="PKN25_13450"
     CDS             1030..1704
                     /locus_tag="PKN25_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV91951.1"
                     /translation="MGFPRAERVLAGFACTVALVASSGTLAAQQSGGASIYCCDVGGQ
                     PVCGDILPAACYGRAYREMSPSGTVRRTVAAPLTPDEIAQRAAAEHKRRAEEAERMRQ
                     LRLDQALLETYRSVQDLDSRRDRELRELDRSIRELRARESELIERQRVMIDEATHTDK
                     SDVAASLEADIRLLDSEIAAQSSVVAAKLRERNAVVERFEEHRRRYVELTSAPESARI
                     PPMPRR"
     gene            1752..3830
                     /locus_tag="PKN25_13455"
     CDS             1752..3830
                     /locus_tag="PKN25_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HNV91952.1"
                     /translation="MPPSTPSEVRVRAACPHDCPDTCAMEVTVADGRAVRIRGAADMP
                     FTNGALCTKVAHYLERVYSERRLLHPMKRVGRKGEGRFERISWDEALDTIAAKYREIA
                     AEDPRAILPYSYCGTMGLVQGESLDRRFFHRLGASLLERTICATAGAHGWKATVGAML
                     GADPEAASEAGLILIWGANPVVSNLHGWRHLQEAKRRGARLVCIDPRRTDTAKKCDLH
                     LAPLPGTDGALALAMMQVLIAEGLLDREYIARHTLGFDALAVRVRACTPEWAAPLTGL
                     AAEAIRSLAIEYGRSARSLIRLNYGLNRCAGGGMAVRNVACLPALTGAWRHPGCGALL
                     STSGNFPVDVQALARPDLYPDARRFPPRALNMSFIGDVLLDSRDPPVRAIHVYNANPV
                     AVAPDSRRVRAGFAREDLFCVVHEQFQTDTADYADILLPATSQLEHRDLHKAYGHPYV
                     VDNRPAIAPLGEARSNTAVFRALARRLGFAAAALYESDDAIAAAAFRADDPRVAGLAE
                     GLQARGWARLALPAGHAPFADGGFRTPSGKCEFFSAALAHEGLDPLPAWHPPAESVPS
                     NPALGARYPLALITPPARNFLNSTFANQARFLADEGGPKLEIHPEDAVARGVVEGMRL
                     RIHNDRGDFIARAVVTTDVRPGVVCSPSVWWPKLCEGGHNANAVIAARPTDMGGGPTF
                     YDCLVEVEPA"
     gene            3827..4618
                     /locus_tag="PKN25_13460"
     CDS             3827..4618
                     /locus_tag="PKN25_13460"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNV91953.1"
                     /translation="MSPSTTLDAVADILARAQRILFVTGAGISVDSGLPTYRGIGGLY
                     HDRLTEEGLTIEEALSGEMMATRPEIAWRYIAQIEASCRGAKPNAAHRLIAALEQEKA
                     LVCVLTQNVDGLHRAAGSRNVIEIHGTVHRLHCMSCGQGRRVEDYAGLSIPPECPRCG
                     GILRPDVVLFGEALPELAIERLESVLSQGMDAVVSIGTTSVFPYIAGPVLWAAREGIP
                     TVEINPGDTQVSQFVDHRLRMCAAEALPELWWRMHPADPAEDRGW"
     gene            complement(4812..>6446)
                     /locus_tag="PKN25_13465"
     CDS             complement(4812..>6446)
                     /locus_tag="PKN25_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNV91954.1"
                     /translation="ITKLDYLTDDEMANFWSYKEKVDPEGRFNRGKLMRKGPIRGNLD
                     NAYTPSFNLMGHESLIMEQTDVGEIAHDIKDCLRCGKCKPVCSTHVPRANLLYSPRNK
                     ILSTSLLIEAFLYEEQTRRGVSLAHWAEFEDVADHCTICHKCEKPCPVDIDFGDVSIK
                     MRNLLRKQGKKSFNPGKAAAMAFLTIKDPTTIKAIRTGMIDWGYKAQRFAHELGKKFG
                     LVQPQVKAPPATLGKAPIKAQVIHFINKPMPGNLPKRTSRALLDIEDDKVIPVIRDPH
                     KANGESEAVFYFPGCGSERLFSQVGLATQAMLYHVGAHTVLPPGYLCCGYPQTAAGEE
                     EKGQKITTDNRVLFHRVANTLNYLDIKTVIVSCGTCMDQLQKYEFEKIFPGCRLLDIH
                     EYLMEKGVKLEGVGGVKYMYHEPCHTPMKQYQGIKVANELMGTRVDLSDRCCGESGTL
                     AVARPDISTQVRFRKQEEIEKGAAKLREGDPNAKIKMLTSCPSCLQGMHRYADDAGGI
                     EPDYIVVELARHLLGENWLPDYVAKANSGGIERVLL"
BASE COUNT          902 a         2299 c         2215 g         1030 t
ORIGIN      
        1 acctgctggc gctcttccga actcgagccc gagggcaggt acagcgacac caccgacagc
       61 ttgccgaagt cgagctgcag gaagcggccc tcggcgtcga tcgcggggat gccgaggccc
      121 tcgacgacgc ggtccggcgc gtgccggctg tagatgccga cgccgctgta gcccttcttc
      181 tcggcggcgt ggaaccagcc ctgcatgccc gcgggcgcgc gcatctcggg gcgcaggtcg
      241 gcgtcctgcg ccttgagttc ctgcacgcac aggaagtcgg cctgctgcgc gggcagccag
      301 tcgagcaggc ccttggtgca ggccgagcgg atgccgttga ggttcagggt gatgatgcgt
      361 aacatcggcg gtttccagtt tcgctgccag gcagccaaag agccgtggat tttagccggg
      421 atttcattgc cctcgcgtgc cgcaagggcg tgctgcgctt cggcgcattc gtcaccaagg
      481 cgggacgcaa ctcgccgtat ttcttcaatg ccggtctgtt cgacgacggc gcctcgttcc
      541 gcgagctgtg cggctactac gcgcgcacca tccgcgccgc gggtatcgcc tgcgacatgc
      601 tgttcgggcc ggcctacaag ggcatcccgc tcgtcgccgg caccgcgatc cggctcgccg
      661 aggacggcgt ggacctgccc ttcgcgttca accgcaagga ggccaaggac cacggcgagg
      721 gcggcacgct gatcggcgcg ccgctgcgcg gcaaggtgct gatcctcgac gacgtgatct
      781 cggccggcac ctcggtgcgc gagtcggtcg agctgatccg cgccgcgggc gccgagcccg
      841 ccggtgtggt gatcgcgctc gatcgcatgg aacgcggtaa aagtgccttg tccgcggtcg
      901 aggaggtcaa ggagaatttc ggtattccgg tcgtcgcggt ggccacgctc gaagacctcg
      961 tcgcctacct ggcggacagt cccgagcttg cggccaacct cgacgcagtc caggcatatc
     1021 gggaacagta tgggatttcc tcgcgctgaa cgcgtcctcg ccggcttcgc ctgcaccgtc
     1081 gcgctggtgg cctcgtccgg cacgctggcg gcgcagcagt ctggcggcgc atcgatctac
     1141 tgctgcgatg tcggcggtca gccggtgtgc ggcgacatcc tgcccgccgc ctgctacggc
     1201 cgcgcctacc gcgagatgag cccctcgggc accgtgcgcc gcacggtcgc ggcgccgctg
     1261 acgcccgacg agatcgccca gcgcgcggcc gccgagcaca agcgtcgcgc cgaggaggcc
     1321 gagcgcatgc gccagctgcg cctcgaccag gcgctgctcg agacctatcg cagcgtccag
     1381 gacctcgaca gccggcgcga tcgcgagctg cgcgaactcg accgcagcat ccgcgagctg
     1441 cgcgcacgcg agtcggagtt gatcgagcgc cagcgcgtca tgatcgacga ggccacgcat
     1501 acggacaaga gcgacgtcgc cgccagcctc gaggccgaca tccgcctgct cgacagcgag
     1561 atcgccgccc agtcctcggt ggtcgcggcc aagctgcgcg agcgcaatgc ggtggtcgag
     1621 cgcttcgagg aacaccgtcg ccgatacgtc gagctcacct cggcgcccga gtccgcgcgc
     1681 atcccgccga tgccgcgtcg ttgagtcttc tgccgtgccg cccggccggg cggccgcaac
     1741 gcgaggcagc gatgcccccc tcgaccccct ccgaagtccg cgtgcgcgcg gcctgcccgc
     1801 acgactgccc cgacacctgc gcgatggagg tcacggttgc cgatggccgc gcggtgagga
     1861 tccgcggcgc cgccgacatg cccttcacca acggcgcgct gtgcaccaag gtcgcgcact
     1921 acctcgagcg ggtgtattcc gagcgccgcc tgctgcatcc gatgaagcgg gtggggcgca
     1981 agggcgaggg gcgcttcgag cgcatctcct gggacgaagc cctcgacacc atcgcggcga
     2041 aataccgcga gatcgccgcc gaggacccgc gcgcgatcct gccctacagc tactgcggca
     2101 ccatgggcct ggtgcagggc gagagcctgg accggcgctt cttccatcgc ctcggcgcct
     2161 ccctgctcga gcgcacgatc tgcgccaccg cgggcgcgca cggctggaag gcgaccgtgg
     2221 gcgcgatgct cggcgccgat cccgaagccg ccagcgaggc cggcctgatc ctgatctggg
     2281 gcgccaaccc ggtggtgtcc aacctgcacg gctggcgcca cctgcaggag gcgaagcggc
     2341 gcggcgcccg cctggtgtgc atcgatccgc ggcgcaccga taccgcgaag aagtgcgacc
     2401 tgcacctcgc cccgctgccc ggcaccgacg gcgcgctcgc gctcgcgatg atgcaggtgc
     2461 tgatcgccga gggcctgctc gaccgcgagt acatcgcccg ccacaccctg ggcttcgacg
     2521 cgctcgccgt acgcgtgcgc gcctgcacgc cggagtgggc ggcgccgctc accggcctcg
     2581 cggccgaggc catccgcagc ctggcgatcg agtacgggcg cagcgcgcgc agcctgatcc
     2641 ggctcaacta cggcctcaac cgctgcgccg gtggcggcat ggcggtgcgc aacgtggcct
     2701 gcctgcccgc gctcaccggc gcgtggcgcc accccggctg cggcgcgctg ctgtcgacct
     2761 cgggcaactt cccggtggat gtgcaggcgc tcgcccgccc cgacctctat cccgacgcga
     2821 ggcgcttccc gccgcgggcg ctcaacatgt ccttcatcgg cgacgtgctg ctcgacagcc
     2881 gcgacccgcc ggtgcgcgcg atccacgtct ataacgccaa cccggtcgca gtcgcgcccg
     2941 acagccgtcg ggtgcgcgcc ggctttgcgc gcgaggacct cttctgtgtg gtgcacgagc
     3001 agtttcagac cgacaccgcc gactacgccg acatcctgct gcccgccacc agccagctcg
     3061 agcaccgtga cctgcacaag gcctacggcc atccctacgt tgtggacaac cggccggcga
     3121 tcgcgccgct cggcgaggcg cgctccaaca ccgcggtctt ccgtgcgctc gcccgccgcc
     3181 tcggctttgc ggctgccgcg ctctacgaaa gcgacgacgc catcgcggcg gcggccttcc
     3241 gcgccgacga cccgcgggtg gcgggcctcg ccgagggcct gcaggcgcgg ggctgggcgc
     3301 ggctcgcgct gcccgcgggc cacgcgccct tcgccgacgg tggcttccgc acgccttcgg
     3361 gcaagtgcga gttcttctcc gccgcgctcg cgcacgaggg cctcgaccct ttgccggcct
     3421 ggcatccgcc cgccgagtcg gtgcccagca acccggcgct gggtgcgcgc tatccgctcg
     3481 cgctgatcac cccgccggcg cgcaacttcc tcaattccac cttcgccaac caggcgcgct
     3541 ttctcgccga cgagggcggg ccgaagctcg agatccaccc cgaggacgcc gtcgcgcgcg
     3601 gcgtcgtgga gggcatgcgc ctgcgcatcc acaacgaccg cggtgacttc atcgcgcgtg
     3661 cggtggtcac caccgacgtg cgccccggcg tggtgtgcag cccctcggtg tggtggccca
     3721 aactgtgcga aggcggccac aacgccaacg cggtcatcgc cgcgcgcccc accgacatgg
     3781 gcggcgggcc gaccttctac gactgcctgg tggaggtgga gcccgcatga gcccgagtac
     3841 gaccctcgac gcggtggcgg acatcctcgc gcgcgcgcag cgcatcctct tcgtcaccgg
     3901 cgccggcatc tcggtcgatt cgggccttcc cacctaccgc ggcatcggcg gcctctacca
     3961 cgaccgcctc accgaggagg gcctcaccat cgaggaggcc ttgtcgggcg agatgatggc
     4021 gacccggccc gagatcgcct ggcgctacat cgcccagatc gaggccagct gccgcggtgc
     4081 gaagcccaac gccgcccacc gcctgatcgc cgcactcgag caggagaagg cgctggtgtg
     4141 cgtgctcacc cagaacgtcg acggcctgca ccgcgccgcc ggttcgcgca acgtcatcga
     4201 gatccacggc accgtgcatc ggctgcactg catgtcctgc gggcaggggc gccgggtgga
     4261 ggactacgcc ggcctctcca tcccgcccga atgcccgcgt tgcggcggca tcctgcgccc
     4321 ggacgtggtg ctgttcggcg aggcgctgcc cgagctcgcg atcgagcgcc tggagagcgt
     4381 gctgtcgcag ggcatggacg cggtggtgtc gatcggcacc accagcgtct tcccctacat
     4441 cgccggcccg gtgctgtggg cggcgcgcga gggcataccc acggtggaga tcaaccccgg
     4501 cgacacccag gtcagccagt tcgtcgacca ccgcctgcgc atgtgcgccg ccgaggccct
     4561 gcccgagctg tggtggcgga tgcatccggc ggatccggcg gaagacaggg gctggtgaag
     4621 gaaacggggc cgtttccgcc aggggcaggc ccgaccgagg gtggagcggg cctcggaaag
     4681 gatcgcgact cgcgtcgctc ctgcaagaat cgcggcgccc ggggtggggc gtaggcgcgg
     4741 cgcaagccgc gacctgacgc tcctccaacg ccgcgcttcc gttcggggcg ccgcgtggag
     4801 gatctccccg cttacagcaa cacccgctcg atccctccgc tgttcgcctt cgccacgtag
     4861 tccggcagcc agttctcgcc gagcaggtgc ctggccagct cgaccacgat gtagtcgggc
     4921 tcgatgccgc cggcgtcgtc ggcgtagcgg tgcatgccct gcaggcagct cggacacgag
     4981 gtcagcatct tgatcttcgc gttcgggtcg ccctcgcgca gcttggccgc gcccttctcg
     5041 atctcttcct gcttgcggaa gcgcacctgg gtggagatgt cggggcgggc caccgcgagc
     5101 gtgccggatt cgccgcagca gcggtcggac aggtcgacgc gcgtgcccat cagctcgttg
     5161 gccaccttga tgccctggta ctgcttcatc ggcgtgtggc agggctcgtg gtacatgtac
     5221 ttcacgccgc ccacgccctc gagtttcacg cccttctcca tcagatactc gtggatgtcg
     5281 agcaggcggc agccggggaa gatcttctcg aattcgtact tctgcagctg gtccatgcag
     5341 gtgccgcagg acacgatgac ggtcttgatg tcgaggtagt tgagcgtgtt ggccacgcgg
     5401 tggaagagca cgcggttgtc ggtggtgatc ttctggccct tctcctcctc gccggcggcg
     5461 gtctgcgggt agccgcagca caggtagccc ggcggcagca cggtgtgcgc gccgacgtgg
     5521 tagagcatgg cctgggtggc cagcccgacc tggctgaaca ggcgctcgga gccgcagccc
     5581 gggaagtaga acaccgcctc ggactcgccg ttggccttgt gcgggtcgcg gatcaccggg
     5641 atgaccttgt cgtcctcgat gtcgagcagc gcgcggctgg tgcgcttggg caggttgccg
     5701 ggcatcggct tgttgatgaa gtggatcacc tgggccttga tcggggcctt gccgagcgtg
     5761 gccgggggcg ccttcacctg cggctggacg aggccgaact tcttgcccag ctcgtgggcg
     5821 aagcgctgcg ccttgtagcc ccagtcgatc atgccggtgc ggatggcctt gatcgtggtc
     5881 gggtccttga tggtcaggaa ggccatcgcc gccgccttgc ccggattgaa cgacttcttg
     5941 ccctgcttgc gcagcaggtt gcgcatcttg atcgacacgt cgccgaagtc gatgtccacc
     6001 ggacagggct tctcgcactt gtggcagatc gtgcagtggt cggccacgtc ctcgaactcg
     6061 gcccagtgcg ccagcgacac gccgcgccgg gtctgttcct cgtacaggaa ggcctcgatc
     6121 agcagcgagg tggagaggat cttgttgcgc ggggaataca gcaggttggc gcgcggcacg
     6181 tgcgtggagc acaccggctt gcacttgccg cagcgcaggc agtccttgat gtcgtgggcg
     6241 atctcgccga catcggtctg ctccatgatc agcgactcgt ggcccatcag gttgaagctg
     6301 ggggtgtagg cgttgtcgag gttgccgcgg atcggcccct tgcgcatcag cttgccgcgg
     6361 ttgaagcggc cttcggggtc gaccttttcc ttgtagctcc agaagttcgc catctcgtcg
     6421 tcggtgagat agtcgagctt ggtgat
//
LOCUS       DAOQBS010000151         6418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_12041_length_6418_cov_15.673896, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000151 DAOQBS010000000
VERSION     DAOQBS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12041_length_6418_cov_15.673896"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PKN25_13470"
     CDS             <1..783
                     /locus_tag="PKN25_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNV91955.1"
                     /translation="PIVQRWIDKWTWRGYRVLTLVAMMMDYMLPKRTMSWKEAWEVYF
                     EENGGALFKDLARYGIKMPKCAEQIALEKDHLSHQAWSTFYNYAAAANFHTWMPDEDE
                     MAWLAAKYPASFDKHYRPRYEFWREQAAKGNRFYNKTLPMLCQTCQIPMIFTEPGDAT
                     KICYREVDYKGDKYHFCSDHCKEIFEHEPEKYVQAWLPVHQIYQGNCFPEGTDPTVEG
                     FDPLAAVLEYYKLEHGRDNLDFDVSEDKKNFAEWRGQATKNI"
     gene            846..1205
                     /locus_tag="PKN25_13475"
     CDS             846..1205
                     /locus_tag="PKN25_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase subunit P4"
                     /protein_id="HNV91956.1"
                     /translation="MSVAALKAYDFPAADTQDKFHGAQLLYIGWEDHLLFCAPFAFPF
                     PPTMRFGDVVAGPMQAAFGYHPDYAQIDWAKAEWFKSGKPFAPDFDKSLAENGLRHKD
                     VIRFRTPGLTGIKGSCS"
     gene            1281..2336
                     /locus_tag="PKN25_13480"
     CDS             1281..2336
                     /locus_tag="PKN25_13480"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenol 2-monooxygenase domain-containing
                     protein"
                     /protein_id="HNV91957.1"
                     /translation="MSYELTIEPIGQTIEVEEGQTILDAALRAGIWLPHACCHGLCAT
                     CKVQVLDGEIDHGEASSFALMDFERDEGKTLACCARLESDVTIEAEIEEDPDAESIPV
                     RDFPGTVTRIEALTPTIKAVFIETDEPMHFQAGQYINFFLEGGEYSRAFSLANPPSTG
                     REVELNIRIVPGGRGTTWVHEQLQVGERVRLSGPYGRFFVRKSAPEALLFMAGGSGLS
                     SPRSMILDLLEAGDTRQMTLVYGQRNRAELYYHDEFLALAAKHSNFTYVPALSDEPAD
                     SGWEGFRGYVHEAATAHFDNDFRGHKAYLCGPPLMIDACITALMQGRLFERDIYMEKF
                     FSAADVQQVRSPLFKKI"
     gene            2356..3270
                     /locus_tag="PKN25_13485"
     CDS             2356..3270
                     /locus_tag="PKN25_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV91958.1"
                     /translation="MSIMSSAQPAAPATAADDPEIGRSIRAAGILTNYHDLGEGFPAL
                     FIHGSGPGVSAWANWRLVLAPLAQRMRVIAPDMAGFGFTERRADLRYDIDTWVAQALG
                     LMDALGLERADVVGNSFGGALALALAIRHPQRVRRLVLMGSVGVHFPITPGLDAVWGY
                     TPSFENMRRIMDCFAWDRSLVNDELAELRYRASVRPGFQESFASMFPAPRQRWVDALA
                     SREEDIRALPHETLIIHGRDDQVIPLQTSETLLRWIERAQLHVFGRCGHWTQIEHSAR
                     FVRLVGDFLCEEGERRAWPAAGRAEGGT"
     gene            3400..4329
                     /locus_tag="PKN25_13490"
     CDS             3400..4329
                     /locus_tag="PKN25_13490"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HNV91959.1"
                     /translation="MAMTGVLRPGHISLRVLDLEEGINFYKNTLGLVETGRDGQGRVY
                     FKAWDEREHNSVLIREAESAGVDFFAFKVADKATLDKLDADLQAFGLKTERIPAGEML
                     ETGERVRFEVPSGHLIELYAEKTDIGNGQSYVNPDPWIPDAERGIAPSRMDHCLLYGP
                     DIEKVQEIFEKVLGFYLVEHVVMEDGKTDLAIWLSCSTKAHDIAFVRHPEPGKLHHIS
                     FKLDSWEKVLRAADIMSMNRISIDIGPTRHGITRGTTIYAFDPSGNRFETFCGGYDTY
                     PDYKTITWTWDEVGAGIFYHDRKLNERFLSVVS"
     gene            complement(4417..5136)
                     /locus_tag="PKN25_13495"
     CDS             complement(4417..5136)
                     /locus_tag="PKN25_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNV91960.1"
                     /translation="MNEAVPQRTPEAVEPKTLVEGAYQRLRRDIIEGVHPPGEKLRVE
                     HLKDRYEVGAGTLREALLLLVTDALVVAQGQRGFRVSPISIEDFEDITRTRLLLETEA
                     LRQSLQLGGEDWEASVVAAFHRLSRAEQKLGDHDSLAAEEWERRNRAFHEALIAASPS
                     RWIRHFQHILYQQSERYRRLSLFSHPIPRDIHAEHQALFNASLARDTTRATSILTEHV
                     LRTLDAVKLLPAEFFAGKHKS"
     gene            5297..5734
                     /locus_tag="PKN25_13500"
     CDS             5297..5734
                     /locus_tag="PKN25_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNV91961.1"
                     /translation="MKVAVPQATISWEAAHAAVAAAVEKAEALGVRINVGVADASGNL
                     VAFLRMPGAFPQSIAIAIDKAYTAGGFGFPTGKWMDVCAGNEGMKLGFSAQPRLVVFG
                     GGLPIRVGGELVGGIGVSGASEDQDGICARAGLLAIGADDVEP"
     gene            5875..>6418
                     /locus_tag="PKN25_13505"
     CDS             5875..>6418
                     /locus_tag="PKN25_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylating acetaldehyde dehydrogenase"
                     /protein_id="HNV91962.1"
                     /translation="MNKIKCALIGPGNIGTDLLYKLKRSPVLEPVWMVGIDATSEGLA
                     RARELGLKTTADGVDGLLPHVKADGVQIAFDATSAYVHAENSRKLNELGVLMIDLTPA
                     AIGPFCVPPVNLVEHVGKGEMNVNMVTCGGQATIPMVAAVSRVQPVAYGEIVATVSSK
                     SAGPGTRKNIDEFTRTTAGAV"
BASE COUNT         1116 a         2173 c         2130 g          999 t
ORIGIN      
        1 ccgatcgtgc agcgctggat cgacaagtgg acctggcgcg gctaccgcgt gctgacgctg
       61 gtcgcgatga tgatggacta catgctgccc aagcgcacga tgagctggaa ggaggcctgg
      121 gaggtgtatt tcgaggagaa cggcggcgcg ctgttcaagg atctcgcgcg ctacggcatc
      181 aagatgccca aatgcgccga gcagatcgcc ctggagaagg atcacctgtc gcaccaggcc
      241 tggagcacct tctacaacta cgccgcggcc gccaacttcc acacctggat gccggacgag
      301 gacgagatgg cgtggctggc ggcgaagtat ccggcgagct tcgacaagca ctaccgtccg
      361 cgctacgagt tctggcgcga gcaggccgcc aagggcaacc gtttctacaa caagacgctg
      421 cccatgctgt gccagacctg ccagatcccg atgatcttca ccgagccggg cgacgcgacc
      481 aagatctgct accgcgaggt cgattacaag ggcgacaagt accacttctg ctccgaccac
      541 tgcaaggaga tcttcgagca cgagccggag aagtacgtcc aggcctggct gccggtgcac
      601 cagatctacc agggcaactg cttccccgag ggcaccgacc cgacggtgga gggcttcgat
      661 ccgctcgccg cggtgctgga gtactacaag ctcgagcacg gtcgcgacaa cctcgacttc
      721 gacgtgtccg aggacaagaa gaacttcgcc gagtggcgcg gccaggccac gaagaacatc
      781 tgagccagcg gtgccgcggg ccggctccgg cccgcggcct tcgacaagac accaggagac
      841 aagacatgag cgttgccgcc ctcaaggcct acgatttccc cgccgccgac acccaggaca
      901 agttccacgg cgcccagctg ctgtacatcg gctgggaaga ccacctgctg ttctgcgcgc
      961 ccttcgcctt ccccttcccg ccgaccatgc gcttcggcga cgtcgtcgcc gggccgatgc
     1021 aggccgcctt cggctaccac cccgactacg cgcagatcga ctgggcgaag gccgagtggt
     1081 tcaagtcggg caagccgttc gcgcccgact tcgacaagtc gctggccgag aacgggctca
     1141 ggcacaagga cgtgatccgc ttccgcacgc ccggcctcac cggcatcaag ggctcctgca
     1201 gctgatcgcc ccccacgggg tgcggcgcgc gccgcacccc gcgcaccccg ccagagaacg
     1261 cacaagaacg gaagcgcacc atgagctacg aactgacgat tgaaccgatc ggccagacga
     1321 tcgaggtgga ggagggccag accatcctcg acgccgccct gcgcgcgggg atctggctgc
     1381 cccacgcctg ctgccacggc ctgtgcgcca cctgcaaggt gcaggtgctc gacggcgaga
     1441 tcgaccacgg cgaggcctcc agcttcgcgc tgatggactt cgagcgcgac gagggcaaga
     1501 cgctggcctg ttgcgcccgc ctcgagtccg acgtgaccat cgaggccgag atcgaggaag
     1561 atcccgacgc cgagtcgatc ccggtgcgcg acttccccgg caccgtgacg cggatcgagg
     1621 cgctgacgcc gacgatcaag gcggtcttca tcgagaccga cgagccgatg catttccagg
     1681 ccggccagta catcaacttc ttcctcgagg gcggggaata cagccgcgcc ttctcgctcg
     1741 ccaacccgcc gtccaccggc cgcgaggtcg agctcaacat ccgcatcgtg cccggcggcc
     1801 gcggcacgac ctgggtccac gagcagcttc aggtcggtga gcgcgtgcgc ctgtccggtc
     1861 cctacggacg cttcttcgtg cgcaagtcgg cgcccgaggc gctgctgttc atggccgggg
     1921 gctcgggtct gtcgagcccg cgctcgatga tcctcgacct gctcgaggcc ggcgacacac
     1981 ggcagatgac cctggtgtac ggccagcgca accgcgccga gctctactac cacgacgaat
     2041 tcctcgccct cgcggcgaag cactccaact tcacctatgt gccggcgctg tccgacgagc
     2101 ccgcggattc gggctgggaa ggcttccgcg gctatgtgca cgaggcggcg acggcacact
     2161 tcgacaacga cttccgcggc cacaaggcct acctgtgcgg cccgccgctg atgatcgacg
     2221 cctgcatcac cgcgctgatg cagggacgcc tgttcgagcg cgacatctac atggagaagt
     2281 tcttctccgc ggcggacgtg cagcaggtac gcagcccgct gttcaagaag atctgagacg
     2341 ggcgaggacg gacggatgag catcatgtcc agcgcgcaac cggcggcgcc agcgaccgct
     2401 gcggacgatc cggagatcgg ccgcagcatc cgcgcggccg gcatcctgac caactaccac
     2461 gacctcggcg agggctttcc cgcgctcttc atccacggct cgggtcccgg cgtctccgcc
     2521 tgggccaact ggcgcctggt gctggcgccg ctcgcgcagc gcatgcgggt gatcgcgccc
     2581 gacatggcgg gcttcggctt caccgagcgg cgtgccgacc tgcgctacga catcgacacc
     2641 tgggtcgcgc aggcgctcgg gctgatggat gcgctcgggc tggagcgggc agacgtggtg
     2701 ggcaactcct tcggcggcgc gctcgcgttg gcgctggcga tccgccaccc gcagcgcgtg
     2761 cgccggctgg tgctgatggg cagcgtgggc gtgcactttc cgatcacgcc ggggctggac
     2821 gcggtgtggg gctacacacc ctcgttcgag aacatgcgcc ggatcatgga ctgcttcgcc
     2881 tgggaccgca gcctggtgaa cgacgagctc gccgaactgc gctaccgcgc cagcgtccgc
     2941 ccgggcttcc aggagtcctt cgcgagcatg ttccccgcgc cgcgccagcg ctgggtcgat
     3001 gcgctggcca gtcgcgagga ggacatccgc gcgctgccgc acgagaccct gatcatccat
     3061 ggccgcgacg accaggtgat tccgctgcag acctcggaga ccctgctacg ctggatcgag
     3121 cgcgcacaac ttcacgtgtt cggccgctgc gggcactgga cgcagatcga gcactccgcg
     3181 cgcttcgtgc gtctggtcgg cgacttcctc tgcgaggagg gcgagcgccg agcctggccg
     3241 gcggcagggc gggctgaagg cggtacttga aggcttcgtg gcgcgcgccc gcagggcggc
     3301 acgggccacg gcaggatccg tcgaacgacg gatgaagatc gagcaaaccg acccgggcgg
     3361 gcgtgccgtc ccgggcatat caagagagga gcagacaaca tggcaatgac aggcgtactt
     3421 cgtccgggac acatctccct gcgtgtcctc gacctcgagg aaggcatcaa cttctacaag
     3481 aacaccctcg gcctggtcga aaccggccgc gacggccagg gccgcgtcta cttcaaggcc
     3541 tgggacgagc gcgagcacaa cagcgtactg atccgcgagg ccgagagcgc cggcgtggac
     3601 ttcttcgcct tcaaggtcgc cgacaaggcg acgctcgaca agctcgacgc cgacctgcag
     3661 gccttcggcc tgaagaccga gcgcataccc gccggcgaga tgctcgagac cggcgagcgc
     3721 gtgcgcttcg aggtgccgtc gggccacctg atcgagctct atgccgagaa gaccgacatc
     3781 ggcaacggcc agtcctatgt gaacccggac ccgtggatcc cggatgccga gcgtggcatc
     3841 gcgccctcgc gcatggacca ctgcctgctg tacgggccgg acatcgagaa ggtgcaggag
     3901 atcttcgaga aggtgctcgg cttctacctc gtcgagcacg tggtgatgga agacggcaag
     3961 accgacctgg cgatctggct gtcgtgctcg accaaggccc acgacatcgc cttcgtgcgc
     4021 caccccgagc cgggcaagct gcaccacatc tcgttcaagc tcgacagctg ggagaaggtg
     4081 ctgcgcgcgg ccgacatcat gtcgatgaac cgcatctcga tcgacatcgg cccgacccgc
     4141 cacggcatca cccgcggcac cacgatctat gccttcgacc cctcgggcaa ccgcttcgag
     4201 accttctgcg gcggctacga cacctacccc gactacaaga ccatcacgtg gacctgggac
     4261 gaggtgggcg ccgggatctt ctatcacgac cgcaagctca acgagcgctt cctgagcgtg
     4321 gtgtcctgac cgcggccgcg ccggcgctcc ctgccgatcg ggcaggggcg ccggtgggcg
     4381 cgcaggcagg ttgaacgggg cgtgcgtccg gcgaactcag gacttgtgct tgccggcgaa
     4441 gaactccgcc ggaagcagct tcaccgcgtc gagcgtgcgc agcacgtgct cggtgaggat
     4501 ggaggtcgcg cgcgtggtgt cgcgtgccag gctggcgttg aacaaggcct ggtgctcggc
     4561 gtggatgtcg cgcgggatcg gatggctgaa caggctcagg cgccggtagc gctcggactg
     4621 ctggtagagg atgtgctgga aatgacggat ccagcgcgag ggcgacgcgg caatcagtgc
     4681 ctcgtggaag gcgcggttgc gccgctccca ctcctcggcg gccagcgagt cgtggtcgcc
     4741 gagcttttgc tcggcgcgag agaggcggtg gaaagccgcg accacggagg cctcccagtc
     4801 ctcgccgccg agctgcagcg actggcgcag ggcttcggtc tcgagcagca ggcgggtgcg
     4861 ggtgatgtcc tcgaagtcct cgatggaaat cggcgagacg cggaagccgc gttgtccctg
     4921 ggcgaccacc agggcgtcgg tgacgagcag gaggagggcc tcgcgcaggg tgccggcgcc
     4981 gacctcgtag cggtccttga gatgttcgac gcgaagtttc tcgccgggcg ggtgaacgcc
     5041 ctcgatgatg tcccggcgca ggcgctggta ggccccctcg acgagggtct tgggctcgac
     5101 ggcctccggt gttctttgcg gaacggcttc gttcatgatt gcgaaaatgt tggaaacacc
     5161 ccggttcccg attctatccg agcgggatgg aattagcttc ttctataaat ctcgagaaaa
     5221 atccttgacg tcgacattgc cctggcctat cttcaaaaac attacaaatc aaacctgaac
     5281 cacaggagct cacccgatga aagtcgctgt tcctcaggcc accatcagct gggaggcggc
     5341 acatgccgcc gtcgccgccg cggtcgagaa ggccgaggcg ctcggggtgc ggatcaacgt
     5401 cggtgttgcc gacgcctccg gcaacctcgt cgctttcctg cgcatgcccg gcgccttccc
     5461 gcagtcgatc gcgatcgcca tcgacaaggc ctacaccgcc ggcggcttcg gcttcccgac
     5521 cgggaagtgg atggacgtgt gcgccggcaa cgagggcatg aagctgggct tctcggcaca
     5581 gccgcggctg gtcgtgttcg gcggcggcct gccgatccgc gtcggcggcg agctggtcgg
     5641 cggcatcggc gtctcgggtg cctccgagga ccaggatggc atctgcgcac gcgcagggct
     5701 gctcgcgatc ggcgccgacg acgtcgagcc ctgagcggcg agttcttttt ttcgtccgca
     5761 ctgcgggcat ctcccggttg cagtcgtgcg gcggtccgtc tctctctccc ctctccccgg
     5821 accgtcgcgc ggcctgcatt cctggaaacg actcaacccc aaaggaagac aggcatgaac
     5881 aagatcaagt gtgcgctgat cggaccgggc aacatcggca ccgacctgct ctacaagttg
     5941 aagcgtagcc cggtgctcga gccggtgtgg atggtgggca tcgacgccac ctccgaaggc
     6001 ctcgcgcgcg cgcgcgagct cggcctcaag accaccgccg acggcgtcga cgggctgctg
     6061 ccgcatgtga aggccgacgg cgtgcagatc gccttcgacg ccacctcggc ctatgtgcat
     6121 gccgagaaca gccgcaagct caacgagctc ggcgtgctga tgatcgacct caccccggcc
     6181 gcgatcggcc ccttctgcgt gccgccggtg aacctcgtcg agcacgtcgg caagggtgag
     6241 atgaacgtga acatggtcac ctgcggcggc caggccacga tcccgatggt cgcggcggtg
     6301 tcgcgcgtgc agccggtggc ctacggcgag atcgtcgcca ccgtgtcctc caagagtgcc
     6361 gggcccggca cgcgcaagaa catcgacgaa ttcacccgca ccactgccgg tgcggtcg
//
LOCUS       DAOQBS010000152         6390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_12121_length_6390_cov_13.916969, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000152 DAOQBS010000000
VERSION     DAOQBS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12121_length_6390_cov_13.916969"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PKN25_13510"
     CDS             complement(<1..721)
                     /locus_tag="PKN25_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV91963.1"
                     /translation="MNTVWIIDDDRSIRWVLEKALGREGIDHASFSSAGDALAELERA
                     PQPPAALLSDIRMPGESGLDLLQKVKERHPQLPVIIMTAYSDLDSAVAAFQGGAFEYL
                     PKPFDVDQAVALVRRALEQTAHQNGASEEATLAPEILGQAPAMQEVFRAIGRLAHSHA
                     TVLINGESGSGKELVARALHRHSPRRDAPFIAINTAAIPRDLLESELFGHERGAFTGA
                     ATQRRGRFEQADGGTLFLDEIG"
     gene            complement(748..1824)
                     /gene="glnL"
                     /locus_tag="PKN25_13515"
     CDS             complement(748..1824)
                     /gene="glnL"
                     /locus_tag="PKN25_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HNV91964.1"
                     /translation="MPSHPTHAASSPSSPFAGLDLLSSAVVLVDAGLVIRYLNAGAEN
                     LFAISRRKLLGQPLERLLGSPPGLAAALDNALRTNWSYTGQDISVQRGDAEPLRLDCT
                     VTPVDTASVRLLLEFRPIDAQLRVAREEQLLHQQQANRELIRNLAHEIKNPLGGIRGS
                     AQLLQHELDDPQLREFTDVIIAEADRLQDLMNRLLSSHCMMRPASINLHDVLERVRRL
                     ILAEFPSIGIVPDYDLSLPELTADREQLIQAVLNIVRNAAQALGGHGEILLRTRIARQ
                     VTLAKRRHKLALKLQVIDDGPGIPEEIRDRIFYPLVSGREGGSGLGLSLAQSFIEQHQ
                     GMIEVDSRPGRTCFTILLPITERA"
     gene            complement(1878..2363)
                     /locus_tag="PKN25_13520"
     CDS             complement(1878..2363)
                     /locus_tag="PKN25_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNV91965.1"
                     /translation="MRPYRILPLLLLAGLAAPAHAEIYKCTDADGRVTYTNDRALARG
                     CVRLQSDQPVSSVPAPVRRPAAPAARETPTGGDFPRVSPNEQRGRDDARRQVLQEELR
                     TEEQKLAEAEKALAEQESIRLGDERNYQKVLDRVRPFKDRVELHKRNVDALRREISGL
                     R"
     gene            complement(2500..3909)
                     /gene="glnA"
                     /locus_tag="PKN25_13525"
     CDS             complement(2500..3909)
                     /gene="glnA"
                     /locus_tag="PKN25_13525"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNV91966.1"
                     /translation="MNAQDVMKMIQENEVRFVDLRFTDIRGKEHHVGLPVSAFEEEHF
                     EHGHPFDGSSLAGWKGIQASDMILMPEPSTAYIDPFFDETTLVITCDVIEPSDGKGYD
                     RDPRSIAKRAEAYLKSTGIGDTAFFGPEPEFFIFDSVEWSVDMSGVYSKIISEEAAWS
                     TADKFEGGNTGHRPRVKGGYFPVPPVDSLNDVRAAMVLALEACGVPVEVHHHEVANAG
                     QCEIGTKFSTLTQRADWTQVLKYIVHNVAHQYGKTATFMPKPIVGDNGSGMHVHQSIW
                     KDGKNLFAGNGYAGLSETALYYIGGIIKHARALNAITNPGTNSYKRLVPHYEAPTKLA
                     YSARNRSASIRIPYVANPKGRRIESRFPDPLANPYMCFAALMMAGLDGIQNKIHPGDP
                     ADKNLYDLPPEEDALIPTVCTSLEQALEYLDKDREFLTRGGVFSNDFIDAYIALKMEE
                     VDRMRITTHPVEFDMYYSL"
     gene            complement(4128..5315)
                     /locus_tag="PKN25_13530"
     CDS             complement(4128..5315)
                     /locus_tag="PKN25_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNV91967.1"
                     /translation="MHSRQASSPVARPSAGADDLARSRGIQRATLVAIVTNTVLTIGQ
                     VVIGLFAGAFSLVADAAHTLSDLITDLLVLLAGRHSADPADTNHPYGHGRIETVTTLV
                     LGSVLAAVGVGFLWSSGIRLQNIGALPVLHPAALGMALLTLAAKEGLFRFSLAAARRL
                     NAPMLEANAWHARSDAASSLVVAAGIGGSLAGYPFLEPLAAAVVGFLILHMGIRLALK
                     SVRELIDTGLPEEELERLRTTIRETPGVIGLHEMRTRRMADRVLCDAHVQVDPRLTVS
                     EGHRVSDAVYLRVRAAHPEVRDVLVHIDPEDDGELQAVPPGPLPERDEILAHMRMLLG
                     PGFADPRRVQIHYLGQRVEVEIILPSLPDADALDALRSHRQTWLAEHPHYRNIRVFVE
                     LAP"
     gene            5375..5827
                     /locus_tag="PKN25_13535"
     CDS             5375..5827
                     /locus_tag="PKN25_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV91968.1"
                     /translation="MGTLSELLTLAHERAESLGLPYQGALTPAETWQVMQLAPGAKLV
                     DVRTRAELDWVGRVPGAIEIEWKSYPSMQENPNFLAQLKHQVDPEALVLFLCRSAVRS
                     DSAARLAGANGYANCYNVLEGFEGDKDANNQRNRIGGWRLAGLPWYQG"
     gene            5945..>6390
                     /gene="nadA"
                     /locus_tag="PKN25_13540"
     CDS             5945..>6390
                     /gene="nadA"
                     /locus_tag="PKN25_13540"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNV91969.1"
                     /translation="MQVSTISFDRFNVLADNEAQERIRAARARLGERAVLLCHHYQRA
                     DVYQHADLTGDSLKLARLASQTDAEYIVFCGVHFMAEVADILSKPSQIAILPDLAAGC
                     SMADMASLAKVERCWRELAEVLGKPDELITPVTYINSAADLKAFCG"
BASE COUNT          957 a         2028 c         2343 g         1062 t
ORIGIN      
        1 cgccgatctc gtcgaggaac agcgtgccgc cgtcggcctg ctcgaagcgg ccgcggcgct
       61 gggtggcggc gccggtgaag gcgccgcgct cgtggccgaa gagttcggat tcgagcaggt
      121 cgcgcgggat ggccgcggtg ttgatggcga tgaagggcgc gtcgcggcgc gggctgtggc
      181 ggtgcagggc gcgcgcgacc agttccttgc cgctgcccga ctcgccgttg atcagcacgg
      241 tggcgtgcga gtgggcgagc cgcccgatgg cgcggaaaac ctcctgcatc gccggggcct
      301 ggccgaggat ctcgggggcc agggtggcct cttcgctcgc accgttctgg tgcgcggtct
      361 gctccagcgc acgacggacg agcgcgaccg cctggtcgac gtcgaagggc ttgggcagat
      421 actcgaaggc gccgccctgg aaggccgcga ctgcgctgtc gaggtcggag taggcggtca
      481 tgatgatgac cggcagctgc gggtggcgct ccttcacctt ctgcagcagg tccagcccgg
      541 actcgccggg catgcggatg tcggatagca gcgcggccgg cggctgcggt gcgcgctcga
      601 gctcggcgag cgcgtcgccg gcggagctga agctggcgtg gtcgatgccc tcgcggccga
      661 gcgccttttc cagcacccag cggatcgagc ggtcgtcgtc gatgatccag acggtgttca
      721 tatcgtatgc actattctgg tgcgcgctca ggcacgctcg gtaatcggca gcaggatcgt
      781 gaagcaggtg cgcccgggac ggctatccac ctcgatcatg ccctggtgtt gctcgatgaa
      841 gctctgtgcg agcgacaggc ccagaccact gccgccctcc cgccccgaaa ccagcggata
      901 gaagatgcga tcgcggatct cctcggggat gccggggccg tcgtcgatta cttgcaattt
      961 gagtgccagt ttgtgacggc gcttggcgag cgtgacctgg cgcgcgatgc gggtgcgcag
     1021 caggatctcg ccgtggccgc ccagcgcctg tgcggcgttg cgcacgatgt tgagcacggc
     1081 ctggatgagc tgctcgcgat cggcggtgag ctcgggcagg ctgaggtcgt agtcggggac
     1141 gatgccgatc gaaggaaact cggcgaggat caggcggcgc acgcgttcga gcacgtcgtg
     1201 gaggttgatc gaggccgggc gcatcatgca atgcgaactc agcaggcggt tcatcaggtc
     1261 ctgcaggcgg tcggcctcgg cgatgatgac gtcggtgaat tcgcgcagct gcgggtcgtc
     1321 gagctcgtgc tgcagcagct gcgccgagcc gcggatgccg ccgagcgggt tcttgatctc
     1381 gtgcgcgagg ttgcggatga gctcgcggtt ggcctgctgc tggtgcagca gctgctcctc
     1441 gcgcgcgacg cgcagctgcg cgtcgatcgg gcggaattcg agcagcaggc gcacgcttgc
     1501 ggtgtcgacc ggcgtcaccg tgcagtcgag ccgcagcggc tcggcgtcgc cgcgctgcac
     1561 gctgatgtcc tggccggtgt agctccagtt ggtgcgcagc gcgttgtcga gcgccgcggc
     1621 caggccgggc gggctgccga gcaggcgctc gagcggttgg ccgagcagct tgcgccggct
     1681 gatcgcgaac aggttctccg cgcccgcgtt gaggtagcgg atcaccagcc cggcgtcgac
     1741 gagcacgacc gccgaagaca gcaggtcgag tccggcgaac gggctgctgg ggctgctggc
     1801 ggcgtgggtg gggtgcgatg gcatgggcgc gtcctgttgt tcggtcgagg acatgcaaga
     1861 agcgcgccag gtccgggtca tcgcaggccg gagatctccc ggcgcagtgc atcgacattg
     1921 cgcttgtgca attcgacgcg gtccttgaag ggtcgcacgc ggtcgagcac cttctggtag
     1981 ttgcgctcgt cgccgagacg gatggattcc tgctcggcga gcgccttctc ggcctcggcc
     2041 agcttctgct cctcggtgcg cagttcttcc tgcagcacct ggcggcgggc atcgtcacgg
     2101 ccgcgctgct cgttggggct cacgcgcggg aagtcgccgc ccgtgggcgt ctcgcgtgcc
     2161 gccggagcgg cgggacggcg cacgggggcg ggcaccgagg acaccggctg gtcggactgc
     2221 aggcgcacgc aaccgcgcgc gagtgcgcga tcgttggtgt aggtcaccct gccgtcggca
     2281 tcggtgcact tgtagatttc ggcatgggcc ggcgcggcaa ggccggcgag caggagcagc
     2341 gggaggattc gatacggtcg catgcgcagg tccggagggg cgcggggacg gcagtaacgg
     2401 gctgcaatgc cgtcatcctt gcgggtcagg cccgattaga ccccagaaaa aagggacggg
     2461 caacgcccgt cccttccgtc ctgcgcagga tttcccggct tacaggctgt agtacatgtc
     2521 gaattccacc gggtgggtgg tgatgcgcat gcgatcgacc tcttccatct tgagcgcgat
     2581 gtaggcgtcg atgaagtcgt tcgagaacac gccgccgcgg gtcaggaact cgcgatcctt
     2641 gtccaggtac tccagcgcct gctcgaggct ggtgcacacg gtcgggatca gcgcgtcctc
     2701 ttccggcggc aggtcgtaca ggttcttgtc ggccgggtcg cccgggtgga tcttgttctg
     2761 gatgccgtcc aggcccgcca tcatcagcgc ggcgaagcac atgtacgggt tggccagggg
     2821 atccgggaag cgcgactcga tgcggcggcc cttcgggttg gcgacgtagg ggatgcggat
     2881 cgaggccgag cggttgcgcg ccgagtaggc gagcttggtc ggggcctcgt agtgcggcac
     2941 caggcgcttg tacgagttgg tgcccgggtt ggtgatcgcg ttgagcgcgc gggcgtgctt
     3001 gatgatgccg ccgatgtagt acagcgcggt ctcggacagg ccggcgtagc cgttgcccgc
     3061 gaacaggttc ttgccgtcct tccagatcga ctggtgcacg tgcatgccgg agccgttgtc
     3121 gccgacgatg ggcttgggca tgaaggtggc ggtcttgccg tactggtggg cgacgttgtg
     3181 cacgatgtac ttgagcacct gggtccagtc ggcgcgctgg gtcagcgtgc tgaacttggt
     3241 gccgatctcg cactggccgg cgttggccac ttcgtggtgg tgcacctcga ccggcacgcc
     3301 gcaggcctcg agtgcgagca ccatggcggc gcgcacgtcg ttgaggctgt cgaccggcgg
     3361 aaccgggaag tagccgccct tgacgcgcgg acggtggccg gtgttgccgc cttcgaactt
     3421 gtcggcggtc gaccaggcgg cctcttccga gatgatcttg ctatacacgc ccgacatgtc
     3481 gaccgaccac tcgaccgagt cgaagatgaa gaactcgggc tcggggccga agaaggcggt
     3541 gtcgccgatg ccggtgctct tcaggtaggc ctcggcgcgc ttggcgatcg agcgcgggtc
     3601 gcggtcgtag cccttgccgt cggagggctc gatgacgtcg caggtgatga ccagcgtggt
     3661 ctcgtcgaag aaggggtcga tgtatgcggt cgacggctcc ggcatgagga tcatgtccga
     3721 ggcctggatg cccttccagc cggccagcga ggagccgtcg aaggggtggc cgtgctcgaa
     3781 gtgctcttcc tcgaaggccg acaccggcag gccgacgtga tgctccttgc cgcgaatatc
     3841 cgtgaagcgc aggtccacga agcgcacttc gttttcctgg atcatcttca tgacgtcttg
     3901 ggcgttcatt ttgactcctg gtgagatgag gcgttgttgc gttgagcggg tggtgcagcg
     3961 gcctggggtc gatgcgccgg attcgtgtga gcagggctaa gcacttagcg tgccagccct
     4021 atgtcagcgc gcagggcatt gtgccacaag gctgcgagcc cgaaatcggt gcctcggccc
     4081 ggtgcggatg cacttcaatg gtgcggatgt gctccctgaa tgcactacta tggtgcgagc
     4141 tccacgaaga cgcggatgtt gcggtagtgc ggatgttccg cgagccaagt ctggcggtgc
     4201 gatcgcagtg catcgagcgc gtccgcatcg gggagggagg gcaggatgat ttccacttcc
     4261 acgcgctggc cgaggtagtg gatctgcacc cgccgagggt cggcgaagcc ggggccgagg
     4321 agcatgcgca tgtgcgcgag gatttcgtcg cgctcgggca gggggccggg cggcaccgcc
     4381 tgcagctcgc catcgtcctc ggggtcgata tgcaccagca cgtcgcgcac ctcggggtgg
     4441 gcggcgcgca cgcgcaggta gaccgcgtcg gacacgcggt gcccctcgga gacggtcagc
     4501 cgtggatcga cctggacgtg ggcgtcgcac agcacgcggt cggccatccg ccgggtacgc
     4561 atttcgtgca ggccgatcac gcccggcgtc tcgcggatcg tcgtgcgcag gcgctcgagc
     4621 tcctcctccg gcaggccggt gtcgatcagt tcgcgcaccg acttcaacgc caggcggatg
     4681 cccatgtgca ggatcaggaa gccgaccacc gccgcggcga gcggctccag gaaggggtag
     4741 ccggccaggc tgccgccgat gccggcggcc acgaccagcg aggacgccgc gtccgagcgt
     4801 gcatgccagg cgttggcctc gagcatcggc gcgttgaggc ggcgtgcggc ggcgagcgag
     4861 aagcggaaca ggccttcctt cgcggcgagg gtcagcagtg ccattcccag ggccgccgga
     4921 tgcagtaccg gcaaggcccc gatgttctgc aggcggatcc ccgaactcca caggaagccg
     4981 acgcccaccg ccgccaggac ggagccgagc accagcgtgg tgacggtctc gatgcggccg
     5041 tgtccgtatg gatggttggt gtcggccgga tcggcgctgt ggcggccggc gagcagcacc
     5101 agcaggtcgg tgatgaggtc ggagagggtg tgggcggcgt cggccaccag gctgaacgcg
     5161 ccggcgaaca ggccgatgac gacctggccg atggtgagca ccgtgttggt cacgatggcc
     5221 accagggtgg cacgctggat gccacggctg cgggcgaggt cgtccgcgcc cgcagaggga
     5281 cgcgcaaccg gggaggaggc ttggcgggag tgcatgaggg gggcgcggcg gacgcgctat
     5341 acttcgaccg ccctcatttt agacggatgt tcgcatggga accctgtcgg aactgttgac
     5401 gctcgcccac gagcgcgccg aaagcctcgg gctgccctac cagggcgcgc tcacccccgc
     5461 cgaaacctgg caggtgatgc agctcgcccc cggtgccaag ctggtcgatg tgcgcacgcg
     5521 cgccgagctc gactgggtgg ggcgcgtgcc cggcgcgatc gagatcgagt ggaagagcta
     5581 tccctcgatg caggagaacc ccaacttcct cgcccagttg aagcaccagg tcgaccccga
     5641 ggcgctggtg ctgttcctgt gccgttccgc cgtgcgctcg gactccgccg cgcggctcgc
     5701 cggcgccaac ggctacgcca actgctacaa cgtgctcgaa ggcttcgaag gcgacaagga
     5761 cgccaacaac cagcgcaacc gcatcggcgg ctggcgcctc gccggcctgc cctggtacca
     5821 gggctgagcg gcaacaaatc cttacccggc cccgcccgcc gcggtcgaac ccgcggcggc
     5881 atgcggggtc cgacgcccgc aaccccggga ttgccccctc ccaacggatc cggataccga
     5941 aacaatgcaa gtctccacca tcagcttcga ccgcttcaac gtgctcgccg acaacgaggc
     6001 gcaggagcgc atccgcgccg cccgcgcacg cctcggcgag cgggcggtgc tgctctgcca
     6061 ccactaccag cgcgccgacg tgtaccagca cgccgacctc accggcgact cgctcaagct
     6121 cgcgcgcctg gcctcgcaga ccgatgccga gtacatcgtg ttctgcggcg tgcacttcat
     6181 ggccgaggtc gccgacatcc tttccaagcc cagccagatc gccatcctgc ccgacctcgc
     6241 cgccggctgc tcgatggccg acatggcgag cctggccaag gtcgagcgct gctggcgcga
     6301 gctcgccgag gtgctcggaa agcccgacga gctcatcacc ccggtcacct acatcaactc
     6361 ggcggccgac ctcaaggcct tctgcggcga
//
LOCUS       DAOQBS010000153         6284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_12451_length_6284_cov_19.061968, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000153 DAOQBS010000000
VERSION     DAOQBS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12451_length_6284_cov_19.061968"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKN25_13545"
     CDS             complement(<1..446)
                     /locus_tag="PKN25_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV91970.1"
                     /translation="MPPVVRLVDVHHRYGDTRAADALTLDLPAGCMVGFIGPDGVGKS
                     TWLGLVAGARRIQRGTVEVLGGDMADARHRARVCPRIAYMPQGLGKNLYPTLSVFENI
                     DFFGRLFGQSAAERRRRIDTLLAATGMAAFADRPANKLSGGMKQKLG"
     gene            complement(554..1621)
                     /locus_tag="PKN25_13550"
     CDS             complement(554..1621)
                     /locus_tag="PKN25_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNV91971.1"
                     /translation="MSMKRSGRLAWLGAGVLVLAAAGAWQFLRPAPDDGSFVRGNGRI
                     EATEVDVAAKAPGRVDAILVNEGDFVTRDDVIARMDVKSLQAQLAQARAEVANAHSAR
                     ETARAQVAQRQADVTMAEAVLVQGRAEYDVARRTAARSQALLNDRATSAQKADDDAAR
                     ARTAEASIHVAEARIVAARAGVRAAEAQVQQAEAAIRAAEAVVARLETEIADGELRAP
                     RSGRVQYRVVQPGEVVGGGGKVVSLVDVGDVYMTFFLPEMAAGRVALGSEVRIVLDAA
                     QAFVIPARVSYVASVAQFTPKTVETQSERQKMVFRVKARIDPELLARYPEQVKTGLPG
                     MAHLRLDAQREWPPALQPRLP"
     gene            1866..2561
                     /locus_tag="PKN25_13555"
     CDS             1866..2561
                     /locus_tag="PKN25_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HNV91972.1"
                     /translation="MSDLAEHARTVRGRGRPRGPDANYRARLLDAALDAFGAQGFEAA
                     GLRAIAASAGCDVSMVAHYFGSKAELWAAVVDRIEAQVRADLAALDMLSPAAGPIERR
                     VVAAVEAMFVQAVRQPQAMRFVMRQMTDPGERLDELVARVVEPVHAVFLPLWQEAIDA
                     GIFRMRSALALQTFVFGAISYAVAAAPVLARMSGGEIDLARLQREFMDGLFARLARGE
                     ADADASDTAEEGR"
     gene            2558..3715
                     /locus_tag="PKN25_13560"
     CDS             2558..3715
                     /locus_tag="PKN25_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV91973.1"
                     /translation="MSAAPRHEARAGEALLAGDGVAAVLRLRGDWTLAHHAALRRQAD
                     ALRGRIDAATRVELAELGTLDTAGARLLHGLLGGERVAALLADEGALSRERRALLKAV
                     ADAIEGREERPAADEGYALGDVLAHIGHTMLVFWRHVSALTGFIGLILETALRVSVQP
                     RRWRVTALVANLERTGLDAVPIIALLTFLVGAVVAFLGATVLQNFGASIFTVDLVAYS
                     FLREFGVLLTAIIVAGRTASSFTAQIGSMRANEEIDAIRVLGLDPVELLVLPRVYALL
                     VALPILTFVAMIAGIVGGMLVSAMTLDISPVLFLSRIEDNVGLIHFWVGMAKAPIFAF
                     LIAVIGCLEGFRVQGSAQSVGEHTTSAVVQSIFVVIVVDAIAALFCMEMGW"
     gene            3712..4548
                     /locus_tag="PKN25_13565"
     CDS             3712..4548
                     /locus_tag="PKN25_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV91974.1"
                     /translation="MTAGLASAASDPRDNIVEVRGVRNQFGAQVVHDQLDLDVRRGEI
                     LSVVGGSGTGKSVLLRTIVGLNRPRAGSVHVFGEDLLSLPDARRKQVERRFGVLFQKG
                     ALFSSLTVAENVALPLIEHAGMSRREAAELAALKIALAGLPALAGDKFPSDLSGGMVK
                     RAALARALALDPDILFLDEPTAGLDPIGAAAFDQLILTLRDALGLTVFIVTHDLDTIY
                     TITDRVAVLAQKKVLVNAGLDEVAATDDAWIREYFHGPRGRAAATAVAAAQAVPAASE
                     NN"
     gene            4550..5491
                     /locus_tag="PKN25_13570"
     CDS             4550..5491
                     /locus_tag="PKN25_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNV91975.1"
                     /translation="METRAHHVLIGLFTVLVVGAALMFALWLGKSDADRQFEVYDIVF
                     QEAVSGLSKGGTVEFNGIKIGDVVSLRLDPADARRVIARVRVDSAAPVRSDTRARLVP
                     AGITGLTMIRLTSGEDPASTPLVSKGDEVARIIAAPSPLSRLLADGEDAITNVNDLLV
                     QARELLSADNVASFGRTLGNLELATGALAAQREDLNAALREVTQASRDASTALAEAAR
                     MLGSANRLVEVQGTQTLDSARDAMKAFERAMGTVDRLIADNRAPLDGGMRGLAEIGPA
                     VAELRTTLASLRIITRQLESRPADYLLGLEPTKEFTP"
     gene            5488..6231
                     /locus_tag="PKN25_13575"
     CDS             5488..6231
                     /locus_tag="PKN25_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNV91976.1"
                     /translation="MRSSSFLPRRGAGPARPRRAFAAAALCAIALLPACSVLPEAAPL
                     DVYVLPVSTGAPGASANVSPGNSIASPAATSTPAGGAPSASAAARALPWSLRVVRPAA
                     GVHLAGQRIVVMPEGNRVSVYQGAGWSDPAPALLRDRLLEAFRADGRVGALSSDERQL
                     HADFELDSDLRAFQSEYRGGRPEAVLRLDARLVHTASRRIVASRTFEQRQPTADPAVP
                     AVVQAFGAAADRLSAEVVEWTVREAAAVR"
BASE COUNT          787 a         2250 c         2304 g          943 t
ORIGIN      
        1 ccgagcttct gcttcatgcc gccggagagc ttgttggcgg ggcggtcggc gaaggcggcc
       61 atgccggtcg cggcgagcag cgtgtcgatg cggcgccggc gctcggctgc ggactggccg
      121 aacagacggc cgaagaagtc gatgttctcg aacaccgaca gggtggggta gaggttcttg
      181 cccaggccct gcggcatgta ggcgatgcgc gggcagacgc gcgcgcggtg gcgggcgtcc
      241 gccatgtcgc cgccgagcac ctcgacggta ccgcgctgga tcctgcgcgc gccggcgacg
      301 aggccgagcc aggtggactt gcccacgccg tcggggccga tgaagccgac catgcagccg
      361 gcggggaggt cgagcgtgag cgcgtcggcg gcacgcgtgt cgccgtagcg gtggtggaca
      421 tcgacgaggc ggacgaccgg tggcagggcg gttgcggccg gtgcgggcga tgcgctgtcc
      481 ggtcgggcgg agggggtgtc cgaggcgccg ggcaaggcgg cccgcgcgcc gttcgtgccg
      541 tcccgtcgag tgctcacggc agcctcggct gcagcgcggg cggccactcg cgctgcgcgt
      601 ccaggcgcag gtgggccatg ccgggcaggc cggtcttgac ctgttcgggg tacctggcga
      661 ggagttcggg gtcgatgcgt gccttgacgc ggaagaccat cttctggcgc tcgctctgcg
      721 tctcgacggt cttgggcgtg aactgggcga cgctggcgac gtaggacacc ctggccggga
      781 tcacgaaggc ctgcgcggcg tcgagcacga tgcgcacctc gctgccgagg gccacgcggc
      841 cggcggccat ctcgggcagg aagaaggtca tgtagacatc gccgacgtcg accaggctca
      901 ccaccttgcc gccaccgccc accacctcgc ccggctgcac cacgcggtac tgcacgcggc
      961 cactgcgagg ggcgcgcagc tcgccgtcgg cgatctcggt ctcgaggcgg gcgaccaccg
     1021 cctcggcggc gcggatcgcg gcctcggcct gctgcacctg ggcctcggcg gcgcgcacgc
     1081 cggcgcgggc ggcgacgatg cgggcctcgg cgacgtggat gctggcctcg gcggtgcgcg
     1141 cacgggcggc atcgtcgtcg gccttctgcg cggaagtggc acgatcgttc agcagcgcct
     1201 gcgagcgcgc cgcggtgcgg cgggcgacgt cgtattcggc gcggccctgc accagcaccg
     1261 cctcggccat ggtcacgtcg gcctggcgct gggcgacctg ggcgcgcgcg gtctcgcggg
     1321 cgctgtgggc gttggcgact tcggcacgcg cctgcgcgag ctgggcctgc agggacttga
     1381 cgtccatgcg cgcgatcacg tcgtcgcgcg tgacgaagtc gccttcgtta accaggatcg
     1441 cgtccacccg cccgggcgcc ttggcggcga catcgacctc ggtggcctcg atgcggccgt
     1501 tgccgcgcac gaagctgccg tcgtccggag cggggcgcag gaactgccag gcgccggccg
     1561 cggccaggac gaggacaccg gcgcccaacc aggccaaccg gccggatcgt ttcatgctca
     1621 tgagattctc ctcgggcgga gctctgcgat gtgttcgtag tatttcaaca cttgttggat
     1681 aatgggtcaa gccggtcaag ctggggtcag gtctttcatt tgctcatctg cggtgggaac
     1741 cggtgcgacc tcgcctttcg tgccgcccga ttcgggccag ccggcgtggc cgccggagag
     1801 attcgaccgg aaccgactcg atgcatgttg taccatcccg cctcgattcc ttctcctttt
     1861 ccagcatgtc cgatcttgcc gaacacgccc gtaccgtgcg cggccgtggc cgtccgcgcg
     1921 gtccggacgc gaactaccgt gcacgcctgc tcgacgctgc cctcgacgct ttcggggcgc
     1981 agggcttcga ggcggcgggg ctgcgcgcga tcgcggcgag cgcgggctgc gacgtgtcga
     2041 tggtggcgca ctatttcggc tccaaggccg aactgtgggc cgcggtggtg gaccgcatcg
     2101 aggcgcaggt gcgcgccgac ctggccgccc tcgacatgct ctctcccgcg gcggggccga
     2161 tcgagcggcg cgtcgtggcc gccgtggagg cgatgttcgt gcaggcggtg cgccagccgc
     2221 aggcgatgcg cttcgtgatg cgccagatga ccgacccggg cgagcgcctg gacgaactcg
     2281 tcgcgcgcgt ggtcgagccg gtgcatgccg tcttcctgcc gctgtggcag gaagcgatcg
     2341 acgcggggat cttccgcatg cgcagtgcgc tcgcgctgca gaccttcgtg ttcggggcga
     2401 tctcgtacgc ggtggcggcg gcgccggtgc tggcgcgcat gagcggtggc gagatcgacc
     2461 tcgcgcgcct gcagcgcgaa ttcatggacg gcctgttcgc ccgcctcgcg cgcggcgaag
     2521 ccgatgcgga cgcatccgac accgcggagg aaggacgatg agcgcggcgc cgcggcacga
     2581 agcccgcgcg ggcgaggccc tgctggccgg cgacggcgtt gccgcggtcc tgcgcctgcg
     2641 cggcgactgg acgctcgccc accatgccgc gctgcgccgc caggccgatg cgctgcgtgg
     2701 ccgcatcgac gccgcgacgc gggtcgagct ggccgagctc ggcaccctcg acaccgcggg
     2761 cgcacgcctg ctgcacggct tgctcggcgg cgagcgcgtc gccgccctgc tcgccgacga
     2821 gggtgcgctg agccgcgagc ggcgcgcgct gctgaaggcc gtggccgatg cgatcgaagg
     2881 ccgcgaggag cgccctgccg cggacgaggg ctacgcgctc ggcgacgtgc tcgcccacat
     2941 cggccacacc atgctggtgt tctggcgcca cgtgagcgcg ctcaccggct tcatcggcct
     3001 gatcctggag actgcgctgc gcgtgagcgt gcagccgcgg cgctggcgcg tgaccgcgct
     3061 ggtcgccaac ctcgagcgca ccggcctcga cgcggtgccg atcatcgcgc tgctgacctt
     3121 cctggtcggc gcggtggtcg ccttcctcgg cgccacggtg ctgcagaact tcggcgccag
     3181 catcttcacg gtcgacctgg tggcctactc gttcctgcgc gagttcggcg tgctgctcac
     3241 cgcgatcatc gtcgccggcc gcacggcgag ctccttcacc gcgcagatcg gctcgatgcg
     3301 cgccaacgag gagatcgacg cgatccgcgt gctcggcctc gatccggtcg agctgctggt
     3361 gctgccgcgc gtgtatgcgc tgctggtcgc gctgcccatc ctgaccttcg tggcgatgat
     3421 cgcgggcatc gtcggcggca tgctggtgtc ggcgatgacg ctggatatct cgccggtgct
     3481 gttcctgtcg cggatcgagg acaacgtcgg gctcatccac ttctgggtcg gcatggccaa
     3541 ggcgccgatc ttcgccttcc tgatcgcggt gatcggctgc ctggaaggct tccgcgtgca
     3601 gggcagcgcg cagtcggtgg gcgagcacac cacttcggcc gtggtgcagt cgatcttcgt
     3661 ggtgatcgtg gtggatgcga tcgcggcgct gttctgcatg gagatggggt ggtgacggcg
     3721 gggctggcga gcgccgcatc cgatccgcgc gacaacatcg tcgaggtgcg cggcgtgcgc
     3781 aaccagttcg gcgcccaggt ggtgcacgac cagctcgacc tcgacgtgcg ccgcggcgag
     3841 atcctcagcg tggtgggcgg ctcgggcacg ggcaagtcgg tgctgctgcg caccatcgtc
     3901 ggcctcaacc ggccgcgcgc gggcagcgtg catgtgttcg gcgaggacct gctgagcctg
     3961 cccgacgcgc gccgcaagca ggtcgagcgc cgcttcggcg tgctgttcca gaagggcgcg
     4021 ctgttctcgt cgctgaccgt ggccgagaac gtggcgctgc cgttgatcga gcacgccggg
     4081 atgtcgcgcc gcgaggcggc ggagctggcg gcgctcaaga tcgcgctcgc cggactgccg
     4141 gcgctcgccg gcgacaagtt tccgtcggat ctgtcgggcg gcatggtcaa gcgtgcggcg
     4201 ctggcgcgcg cgctcgcgct cgaccccgac atcctcttcc tcgacgaacc caccgccggc
     4261 ctcgacccga tcggcgccgc ggccttcgac cagctcatcc tcaccctgcg cgacgcgctc
     4321 gggctcacgg tgttcatcgt cacccacgac ctcgacacca tctacaccat caccgaccgc
     4381 gtcgccgtgc tggcgcagaa gaaggtgctg gtcaacgccg gtctggacga ggtcgccgcc
     4441 accgacgacg cctggatccg cgaatacttc cacggcccgc gcggccgcgc ggccgccacc
     4501 gcggtggccg ccgcgcaggc cgtgcccgct gcatcggaga acaactgaca tggaaacccg
     4561 cgcccatcat gtgctgatcg gcctcttcac cgtgctcgtc gtcggcgccg cgctgatgtt
     4621 cgcgctgtgg ctgggcaaga gcgacgccga ccgccagttc gaggtgtacg acatcgtctt
     4681 ccaggaggcg gtctccggcc tgtccaaggg cggcacggtg gagttcaacg gcatcaagat
     4741 cggcgacgtg gtcagcctgc gcctcgatcc cgccgatgcg cgccgcgtca tcgcccgcgt
     4801 gcgcgtggac agcgccgcgc cggtgcgcag cgacacccgc gcccgcctgg tgcctgccgg
     4861 catcaccggc ttgaccatga tccgcctgac cagcggcgag gatccggcga gcacgcccct
     4921 ggtgtccaag ggcgacgagg tggcgcgcat catcgccgcg ccttcgccgc tgagccgcct
     4981 gctcgccgac ggcgaggacg cgatcaccaa cgtgaacgac ctgctggtgc aggcgcgcga
     5041 gctgctctcg gccgacaacg tggcctcgtt cgggcgcacg ctgggcaacc tggagctggc
     5101 caccggcgcg ctcgccgcgc agcgcgagga cctcaacgcc gcgttgcgcg aggtcaccca
     5161 ggccagccgc gacgccagca cggcgctcgc cgaggccgcc cgcatgctcg gctcggccaa
     5221 caggctggtg gaggtgcagg gcacgcagac cctggacagc gcacgcgacg cgatgaaggc
     5281 cttcgagcgc gcgatgggca cggtcgaccg cctgatcgcc gacaaccgcg cgccgctcga
     5341 cggcggcatg cgcggcctgg ccgagatcgg ccccgcggtg gcggagctgc gcaccacgct
     5401 ggcttcgctg cgcatcatca cccgccagct cgagagccgt cccgccgact acctgctcgg
     5461 cctcgaaccg accaaggagt tcaccccgtg agatcgtcgt ccttccttcc gcgccgcggc
     5521 gccggccccg cgcgcccgcg tcgggcgttc gccgcggcgg cgctgtgcgc gatcgcgctg
     5581 ctgccggcct gcagcgtgct gcccgaggcc gcgccgctcg acgtgtatgt gctgccggtg
     5641 tcgacgggcg cgcctggtgc ttcggcgaac gtgtccccgg gcaactccat cgccagccct
     5701 gcggcgacgt ccactcccgc gggcggcgcc ccgtccgcct ccgcagccgc ccgcgcgctg
     5761 ccatggtcgc tgcgcgtcgt gcgtccggcc gccggcgtgc atctggccgg ccaacgcatc
     5821 gtggtgatgc cggagggcaa ccgggtgagt gtctaccagg gcgcgggctg gagcgatccg
     5881 gcgcccgcgc tgctgcgcga ccgcctgctc gaagccttcc gcgccgacgg ccgggtgggt
     5941 gcgttgagca gcgacgaacg ccagctgcat gccgatttcg agctcgacag cgacctgcgc
     6001 gccttccaga gcgaataccg tggcggccgc cccgaggcgg tgctgcgcct cgacgcgcgc
     6061 ctggtgcaca ccgccagccg ccgcatcgtc gccagccgga ccttcgagca gcgccagccc
     6121 accgcggacc ccgccgtgcc ggccgtcgtc caggccttcg gcgccgccgc cgaccgcctg
     6181 tcggccgagg tggtggagtg gaccgtgcgc gaggctgcgg cggtgcgctg agcccggagc
     6241 gtcggatcgc gactcgcgtc gctcccacaa gaaaccgccg cgcc
//
LOCUS       DAOQBS010000154         5995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_13366_length_5995_cov_12.880303, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000154 DAOQBS010000000
VERSION     DAOQBS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13366_length_5995_cov_12.880303"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PKN25_13580"
     CDS             <1..1022
                     /locus_tag="PKN25_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV91977.1"
                     /translation="PLVLKDKIAARIKVISRLDISEKRVPQDGRMKLVLSRNKAIDFR
                     VSTLPTLHGEKIVMRILDPSSAMLGIDALGYDPEQREALLAAVERPYGMILVTGPTGS
                     GKTVSLYTCLNLLNKPGVNISTAEDPAEINLAGINQVNVNEKAGLTFSAALRAFLRQD
                     PDVIMVGEIRDIETAEIAIKAAQTGHLVMSTLHTNDAPSTLERLKNMGVAPFNIASSV
                     ILITAQRLARRLCSCKKPVDIPAQALLEAGFEPDELDGGWQPMGPGGCERCKGSGYKG
                     RVGIYQVMPISEEIARIIMTGGNSMDIAAQARSEGVLDLRQSGLRKVRQGITSLEEVL
                     ATTNE"
     gene            1038..2267
                     /locus_tag="PKN25_13585"
     CDS             1038..2267
                     /locus_tag="PKN25_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV91978.1"
                     /translation="MATASRAARATGPREDLYIWEGKDKTGKVVRGEIRATGDAMVQA
                     MLRRQGIQVIKIRKQKMARGGRISEKDIALFTRQLATMMKSGVPLLQAFDIAMKGSGN
                     AALVRLLNDIRTDVETGSNLSQAFAKHPAHFDRLFCNLVAAGEQAGILDGLLDRIATY
                     KEKILAIKSKIKSALFYPAAVVVVAGLVISVMMLFVIPEFKSVFSSFGAELPVPTLMV
                     IAISDAFVAYWYLIFGALIAALVAIAWSYKRSTAMQIAVDRLVLRIPVIGAIIRKATV
                     ARWTRTLSTMFAAGVPLVEALDSVGGASGNHVYVVATHQIQSEVSTGTSLTVAMQNAE
                     VFPTMVVQMVSIGEESGQLDAMLGKVADFFEQEVDDAVAGLSQLLEPLIMVFLGTIIG
                     GLVVAMYLPIFKLGSVV"
     gene            2272..3123
                     /locus_tag="PKN25_13590"
     CDS             2272..3123
                     /locus_tag="PKN25_13590"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNV91979.1"
                     /translation="MVELLRDPLAFTILSGLVGLFVGSFLNVVIHRLPRMMERDWHAQ
                     AAELRGEQAPANERFNLATPRSRCPHCGHQITMLENIPIVSYVVLRGRCAHCGAGISR
                     RYPIVEALSALLSAYAAWHFGFGASALGALLFVWAMVALTFIDLDTQLLPDDITLPLL
                     WLGLAFNLGTTFAELPDAVIGAMAGYLALWSVFWLFKLATGKEGMGYGDFKLLAAIGA
                     WLGWQMLPLTILLSSLVGAVVGILLIVVARHGRNVPIPFGPYLAAAGLIALFWGERIT
                     ERYLGLL"
     gene            3281..3544
                     /locus_tag="PKN25_13595"
     CDS             3281..3544
                     /locus_tag="PKN25_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV91980.1"
                     /translation="MPIYEYRCPSCGFQKEHLQKMSDEPLSVCPSCGTTGYAKQLSAA
                     GFQLKGSGWYATDFKGGAKSAAPAAESTPSPATGGCGGGCACH"
     gene            3627..4253
                     /locus_tag="PKN25_13600"
     CDS             3627..4253
                     /locus_tag="PKN25_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNV91981.1"
                     /translation="MKKYFITGLLIWLPLAITYMVIAWIVGTLDAILLWLPAEYQPSR
                     YIGFDIPGVGVVASLLLVFFTGLVAANVLGQKLVQLWEALLARIPVVKSIYYSVKQVS
                     DTVFSSNGQAFRKALLVQYPREGVWTIAFLTGQPGGDAAEHLRGDYVSVYVPTTPNPT
                     SGFFLMMRRSEVVELDMSVDDALKYIISMGVVAPPARRPEPVPALSDT"
     gene            4341..>5995
                     /gene="aspS"
                     /locus_tag="PKN25_13605"
     CDS             4341..>5995
                     /gene="aspS"
                     /locus_tag="PKN25_13605"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNV91982.1"
                     /translation="MRTHYCGKVTAADLDQIVTICGWVHRRRDHGGVIFIDLRDREGL
                     VQVVCDPDRAEMFKTAESVRNEFVLKLSGKVRRRPAGTENANLVSGEIEILCHDIEVL
                     NAAATPPFQLDDENLSETVRLTNRVIDLRRPQMQKNLMLRYKTTMAFRRFLDGAGFID
                     VETPMLTKSTPEGARDYLVPSRVHPGQFFALPQSPQLFKQLLMVAGYDRYYQIVKCFR
                     DEDLRADRQPEFTQVDLETSFMNEHEITALVEEMIRYVFKEAMAVELPAPFPRMTYAE
                     AMRRYGSDKPDLRVTLELTDVTDAVQDVAFKVFSGPATSGGRVAAMRVPGGNSLTRGE
                     IDEYTKFVAIYGARGLAYIKVNDVTQPNEHGLQSPIVKNLHETALRTILERTGAQSGD
                     LIFFGADKTKVVNDAMGALRIKLGHEKGYVTGEAWRPLWVVDFPMFEYDEDDKRWTAC
                     HHPFTSPKDEHIDLLESNPGECLAKAYDLALNGWEIGGGSVRIHRADVQARVFEALNI
                     GPEEQQAKFGFLLDALKYGAPPHGGLAFGLDRIVTMMTGAESIR"
BASE COUNT         1005 a         2162 c         1863 g          965 t
ORIGIN      
        1 cgccgctggt gctcaaggac aagatcgccg cgcgcatcaa ggtgatctcg cgcctcgaca
       61 tctcggagaa gcgcgtgccg caggacgggc gcatgaagct cgtgttgtcg cgcaacaagg
      121 cgatcgactt ccgcgtctcg acgctgccga ccctgcacgg cgagaagatc gtgatgcgca
      181 tcctcgatcc gagctcggcg atgctcggca tcgacgcgct cggctacgac cccgagcagc
      241 gcgaggcgct gctcgccgcg gtcgagcgcc cctacggcat gatcctggtc accggcccca
      301 ccggctcggg caagacggtg tcgctgtaca cctgcctcaa cctgctcaac aagccgggcg
      361 tgaacatctc caccgccgag gaccctgccg agatcaatct cgccggcatc aaccaggtca
      421 acgtcaacga gaaggccggc ctcaccttct ccgccgcgct gcgcgccttc ctgcgccagg
      481 acccggacgt gatcatggtg ggcgagatcc gcgacatcga gaccgccgag atcgccatca
      541 aggccgcgca gaccggccac ctggtgatgt ccaccctgca caccaacgac gcgccctcca
      601 cgctcgagcg cctcaagaac atgggcgtgg cgccgttcaa tatcgcatcc tcggtgatcc
      661 tgatcacggc ccagcgcctc gcccgccggc tatgctcctg caagaagccc gtcgacattc
      721 ccgcccaggc cctgctggag gccggcttcg agcccgacga gctcgacggc ggctggcagc
      781 cgatgggacc gggcggttgc gaacgctgca agggcagcgg ctacaagggc cgggtgggca
      841 tctaccaggt catgccgatc agcgaggaga tcgcccgcat catcatgacc ggcggaaact
      901 ccatggacat cgccgcccag gcccgcagcg agggcgtgct cgacctgcgc cagtcgggcc
      961 tgcgcaaggt gcgccaaggc atcacctcgc tcgaagaagt gctggcgacg accaacgaat
     1021 aaaggacgct cgatttcatg gccactgcaa gccgcgcggc acgtgccacc ggtccgaggg
     1081 aggaccttta tatctgggaa ggcaaggaca agaccggcaa ggtcgtccgc ggcgagatcc
     1141 gcgccaccgg cgacgccatg gtccaggcca tgctgcgccg ccagggcatc caggtgatca
     1201 agatccgcaa gcagaagatg gcgcgcggcg gacgcatctc cgaaaaggac atcgcgctgt
     1261 tcacccgcca gctcgccacg atgatgaaat ccggtgtgcc gctgctgcag gccttcgaca
     1321 tcgcaatgaa aggctccggc aacgccgcgc tcgtgcgcct gctcaacgac atccgcaccg
     1381 acgtcgagac cggctccaac ctgtcgcagg ccttcgccaa gcaccctgcg cacttcgacc
     1441 ggctgttctg caacctggtc gcagcgggcg agcaggcggg catcctcgac ggcctgctcg
     1501 accgcatcgc cacctacaag gaaaagatcc tcgcgatcaa gagcaagatc aagtcggcgc
     1561 tgttctatcc tgccgcggtg gtcgtggtgg cgggtctcgt gatctcggtg atgatgctgt
     1621 tcgtgatccc cgagttcaag agcgtgttct ccagcttcgg tgccgagctg ccggtgccca
     1681 ccttgatggt catcgccatc tcggacgcct tcgtggccta ctggtacctg atcttcggcg
     1741 ccctcatcgc ggccctcgtg gcgatcgcct ggagctacaa gcgctcgact gcgatgcaga
     1801 tcgcggtcga ccggctggtg ctgcgcatcc cggtgatcgg cgcgatcatc cgcaaggcca
     1861 cggtcgcgcg ctggacgcgc acgctgtcga ccatgttcgc ggccggcgtg ccgctggtcg
     1921 aggcgctcga ctcggtggga ggcgcatcgg gcaaccacgt ctatgtggtg gcgacacatc
     1981 agatccagag cgaggtcagc accggcacca gcctgacggt cgcgatgcag aacgcggaag
     2041 tctttcccac catggtggtg cagatggtgt cgatcggcga ggagtccggc cagctcgacg
     2101 ccatgctggg caaggtcgcc gatttcttcg agcaggaggt ggacgacgcg gtcgccggcc
     2161 tgtcgcaact gctcgagccg ctgatcatgg tcttcctcgg caccatcatc ggcggcctgg
     2221 tggtggcgat gtacctgccg atcttcaagc tcggttcggt cgtctgaacc catggtcgag
     2281 cttctccgcg acccgctcgc cttcaccatc ctctccggcc tggtcggact cttcgtcggc
     2341 agcttcctca acgtcgtgat ccatcggctg cccaggatga tggagcgcga ctggcacgcc
     2401 caggcggccg agctgcgtgg cgagcaagca cccgccaacg agcgcttcaa cctcgccacg
     2461 ccgcgctcgc gctgcccgca ctgcgggcac cagatcacga tgctggagaa catcccgatc
     2521 gtcagctacg tggtgctgcg cgggcgctgc gcacattgcg gcgccggcat cagcaggcgc
     2581 tacccgatcg tcgaagccct ctccgcgctg ctgtcggcct acgccgcctg gcatttcggc
     2641 ttcggcgcca gcgcgctcgg ggccctgctg ttcgtgtggg cgatggtcgc gctcaccttc
     2701 atcgacctcg acacccagct gctgcccgac gacatcaccc tgccgctgct gtggctgggc
     2761 ctcgccttca acctcggcac caccttcgcc gagcttcccg acgcggtgat cggcgcgatg
     2821 gcgggctacc tcgcgctgtg gtcggtgttc tggctgttca agctcgccac cggcaaggag
     2881 ggcatgggct acggagactt caagctgctc gccgcgatcg gcgcctggct gggctggcag
     2941 atgctgccgc tgaccatcct gctgtcctcg ctggtgggcg cggtggtcgg catcctgctg
     3001 atcgtcgtcg cccgccacgg ccgcaacgtg ccgatcccct tcggccccta cctcgccgcg
     3061 gccggcctga tcgcgctgtt ctggggcgag cgcatcaccg agcgctacct cgggctgctc
     3121 tgagcgcgcg acgcgcgcgc cgctgcggac ggtctgccgc ggggggcttg aagccctcct
     3181 cccccgccct catcttgcac ccgggcgcga ccgttccgga cgcgcttttt ccttatgctg
     3241 cgttgcgtta gacttgcgca cttgttttcg ggagcacgcc atgcccatct atgaatatcg
     3301 ttgcccgagc tgtggtttcc agaaggaaca cctgcaaaaa atgagcgacg agcccctgtc
     3361 cgtctgcccg tcctgcggca cgaccggcta cgccaagcag ttgtcggcgg ccggcttcca
     3421 actcaagggc tcgggctggt acgccaccga cttcaagggc ggagccaaga gcgcagcccc
     3481 ggcggccgaa tccacgccct cgcctgcgac cggcggctgc ggcggcggct gcgcctgcca
     3541 ctgagcggaa cgacccatct cgcggcgggc cggccgcggc gcggcagacg caagcagccg
     3601 cgcagcgcgg cggccaggaa ccccacttga aaaaatactt catcaccggc ctgctgatct
     3661 ggctcccgct ggcgatcacc tacatggtga tcgcctggat cgtcggcacg ctcgacgcga
     3721 tcctgctgtg gctgcccgcc gaataccagc cgagccgcta catcggcttc gacatccccg
     3781 gcgtgggcgt cgtcgcgagc ctgctgctgg tgttcttcac cggcctggtc gccgccaacg
     3841 tgctcgggca gaagctcgtg cagctctggg aagcgctgct tgcccgcatc ccggtcgtca
     3901 agtcgatcta ctacagcgtc aagcaggtct ccgacacggt gttctcgagc aacggccagg
     3961 ccttccgcaa ggcgctgctg gtgcaatacc ctcgcgaggg cgtgtggacc atcgccttcc
     4021 tcaccggcca gccgggcggc gacgcggccg agcacctgcg cggcgactac gtcagcgtgt
     4081 atgtaccgac cacgcccaac ccgacctcgg gcttcttcct gatgatgcgg cgcagcgagg
     4141 tcgtcgagct cgacatgagc gtggacgacg cgctcaagta catcatctcg atgggcgtgg
     4201 tcgctccgcc ggcgcgcagg ccggaacccg tgcccgcgct gtccgacacc tgaacgaagc
     4261 tccccgaaca ctccagcctc tccaccctgc cgaatcctga atccccgggc gcaagcgccc
     4321 ccgaacctgc ggaacccgac atgcgaactc actactgcgg caaagtcacc gccgccgacc
     4381 tcgaccagat cgtcaccatt tgcggctggg tacatcgccg ccgcgaccac ggcggcgtca
     4441 tcttcatcga cctgcgcgat cgcgagggcc tggtgcaggt ggtgtgcgac cccgaccgcg
     4501 cggagatgtt caagacggcc gaatcggtgc gcaacgagtt cgtcctcaag ctgagcggca
     4561 aggtgcgccg ccgtcccgcc ggcaccgaga acgccaacct cgtctcgggc gagatcgaga
     4621 tcctctgcca cgatatcgag gtgctcaacg ccgccgccac gccgcccttc cagctcgacg
     4681 acgagaacct gtccgagacg gtgcgcctga ccaaccgcgt catcgacctg cgtcgcccgc
     4741 agatgcagaa gaacctgatg ctgcgctaca agaccacgat ggccttccgc cgcttcctcg
     4801 acggcgccgg cttcatcgac gtcgagacgc cgatgctgac caagagcacg cccgaaggcg
     4861 cgcgcgacta cctggtgccc tcgcgcgtgc acccgggcca gttcttcgcc ttgccgcagt
     4921 cgccccagct cttcaagcag ctgctgatgg tggccggcta cgaccgctac taccagatcg
     4981 tcaagtgctt ccgcgacgag gacctgcgcg ccgaccgcca gcccgaattc acccaggtcg
     5041 acctcgagac ctcgttcatg aacgagcacg agatcaccgc cctggtcgaa gagatgatcc
     5101 gctacgtgtt caaggaagcg atggcggtcg agctgcccgc gcccttcccg cgcatgacct
     5161 acgccgaggc gatgcgccgc tacggctcgg acaagcccga cctgcgcgtg acgctggaac
     5221 tcaccgacgt caccgacgcg gtgcaggacg tcgccttcaa ggtcttcagc ggccccgcca
     5281 cctcgggcgg ccgcgtcgcc gcgatgcgcg tgccgggcgg caacagcctg acccgcggcg
     5341 agatcgacga atacaccaag ttcgtcgcca tctatggcgc ccgcggcctc gcctacatca
     5401 aggtcaacga cgtcacccag cccaacgagc acggcctgca gtcgccgatc gtcaagaacc
     5461 tgcacgagac cgcgctgcgc accatcctcg agcgcaccgg cgcgcagtcg ggcgacctga
     5521 tcttcttcgg cgccgacaag accaaggtgg tcaacgacgc catgggcgcg ctgcgcatca
     5581 agctcggcca cgagaagggc tacgtcaccg gcgaggcctg gcgtccgctg tgggtggtcg
     5641 acttcccgat gttcgagtac gacgaggacg acaagcgctg gaccgcctgc caccacccct
     5701 tcaccagccc caaggacgag cacatcgacc tgctcgaatc caacccgggc gagtgcctgg
     5761 ccaaggccta cgacctcgcc ttgaacggct gggagatcgg cggcggctcg gtgcgtatcc
     5821 accgcgccga cgtgcaggcg cgcgtgttcg aggcactgaa catcggcccc gaggagcagc
     5881 aggccaagtt cggcttcctg ctcgacgcgc tcaagtacgg cgcccccccg catggcggcc
     5941 tggccttcgg cctcgaccgc atcgtcacca tgatgaccgg cgccgaatcc atccg
//
LOCUS       DAOQBS010000155         5952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_13498_length_5952_cov_18.063083, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000155 DAOQBS010000000
VERSION     DAOQBS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13498_length_5952_cov_18.063083"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..1359
                     /locus_tag="PKN25_13610"
     CDS             13..1359
                     /locus_tag="PKN25_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNV91983.1"
                     /translation="MKSALFVDFDNVYSGLRKLDPAIADRFARQPLEWVNWVIGALEL
                     PDHAPAGARRRLLVRRCYLNPQAYQRFRPSFNLAGFEIIDCPALTSEGKTSTDIHMVL
                     DIIDLLQHEARYDEFIVFSADADFTPVLRKLRRWDRRTTVLAIGFPSAAYRASADLLI
                     DPDEFVRNALGFKDEDEAPGAGAPPADVAPESLSLAASARFAQASPEAARSVPPASLS
                     SGADALDHDLAEIVETMRVEVARSAAPVPCSRLASLITTRHPALAADWNGKGSFRRFV
                     DSLDLAPLRFDWSNSGGFVFDPARSGAGMQDGNATPDWGEDQDILPLAMQIHEVSGVP
                     LLGPAEYRALFSIIEDDVGRHPFDLKETGKRVRDRLREAGLGGSRLDVNWILRGLLMR
                     GHAFGEGQDRAATLATKTANNVRSLCLREQMILDRAAETAIARWFGTHPAPTRSGQ"
     gene            1451..2005
                     /locus_tag="PKN25_13615"
     CDS             1451..2005
                     /locus_tag="PKN25_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HNV91984.1"
                     /translation="MASRNPSFFRRIGLAFSVLFGAELAGRLVRLREEGADTEAAAGT
                     EDAAAAAQPAIVRETTPEAALQLLGLLQRDARFIDFVQEDIAAYSDADIGAAARVVHE
                     GCRKVLDEHFAIAPVRDEAEGSRLTLPEGFDAAAVRLTGKVVGKPPFTGTLGHRGWRV
                     TDTRLPALAEGHDASVIAQAEVEL"
     gene            2002..3849
                     /locus_tag="PKN25_13620"
     CDS             2002..3849
                     /locus_tag="PKN25_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNV91985.1"
                     /translation="MSAPARYTLGIDLGTTHCALSYVDLEASDGDQAAQQVLEIPQLT
                     GPGSIEARPLLPSFLYLPHADELAPGDLSLPWGATQDHAVGEFARSRGAATPIRLVAS
                     AKSWLCHPGVDRRADILPAEAPPEVARISPLTASVRYLSHLRAAWNAAHPEAPFDAQD
                     VTVTIPASFDPAARELTAEAAQAAGYPPITLLEEPQAALYSWIQRSAGQWRKEVKTGD
                     LILVVDVGGGTTDLSLIAVLEREGNLELHRVAVGEHILLGGDNMDLALAYTVTRKLAE
                     QGTRLDPWQTRALAHACRGAKEALLSDSDLDSVPVVVPSRGSKLIGGSIRTEVRREEL
                     VATLVEGFFPRVAVTEMPITRARAALTQLGLPYAQDAAVTRHLAAFLTRQLGATADLE
                     GFAAQPEESVFLHPTAVLFNGGVLKSGLIAGRVLDTLNDWLAADGAAPARLLGGADLD
                     LAVARGAAYYGYVRRGRGVRIRGGTARAYYVGIESSMPAIPGMEPPLEALCVAPFGME
                     EGSEAALPAQEFGLVVGEPVRFRFFGSSVRRQDEVGTLLDFWQPDELQELEEIEATLP
                     AEGRRPGEVVRVKLHARVTETGTLELEALPVDGSARWKVQFDVRGNAEA"
     gene            3860..>5952
                     /locus_tag="PKN25_13625"
     CDS             3860..>5952
                     /locus_tag="PKN25_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNV91986.1"
                     /translation="MATRYTVGIDLGTSNTVVAFAAEGEDRIQVLEIEQLVGRGELAA
                     RTLLPSVRYHPAAGELDPATLRLVASAGEGQGAPPAVLGEAVVGQYARELGAQVPGRA
                     VASAKSWLSHAGVDRNAAILPWGAAEDVPRVSPVVASASYLAHVRAAWNRQHPGAALE
                     NQDIVLTVPASFDEGARALTLEAARLAGLPRLRLLEEPQAAFHDWLFRHREQLAAELA
                     QSRLLLVCDIGGGTTDLTLIQVEPAADGLSPPRLTRIGVGDHLMLGGDNMDLALAHLV
                     EKRLLAGGERLSAAQLAQLVQRCRAAKEQLLAADAPEELKVTLLGAGSRLIGAARSTA
                     LRRDEVLRLVVDGFLPLVGADEAPARRRAAIVEFGLPYPADPAISRHLAAFLRRHGQV
                     SRVALGMPGTGAGRDADGDADEAAAPLAIPDTLLLNGGVFRADAISARLEGLLAHWRG
                     APPRVLHNDAPDLAVARGAVAFALVRAARSGPGIGGGSARSYFLVLEAGGEAARAICV
                     LPRGTESGTEVALPERRFALRLGQPVRFHLVSSTGDRAWQAGELVELDADDFIHLPPL
                     AARLPATAAGRRENVAVRLVACLTEVGTLEMQCVAIDDDTQRWQLAFQVRAEADMPAT
                     EAAAAAGAHPRLAQAIALIDEVFGGQARELDAKAVRQLRARLERVLGRRDDWDMALLR
                     ALFDALLERARRRRRS"
BASE COUNT          772 a         2397 c         2010 g          773 t
ORIGIN      
        1 ccgcgagaca ccttgaaaag cgccctcttc gtcgacttcg acaacgtcta ttccggcctg
       61 cgcaagctcg accccgccat cgccgaccgc ttcgcccgcc agcccctgga gtgggtgaac
      121 tgggtgatcg gcgcgctcga gctgcccgac cacgcccccg ccggtgcgcg ccgccggctg
      181 ctggtgcggc gctgctacct caacccgcag gcctaccagc gcttccgccc gtccttcaac
      241 ctcgccggct tcgagatcat cgactgcccc gcgctgacga gcgagggcaa gaccagcacc
      301 gacatccaca tggtgctcga catcatcgac ctgctgcagc acgaggcccg ctacgacgag
      361 ttcatcgtgt tctcggccga cgccgacttc accccggtgc tgcgcaagct gcggcgctgg
      421 gatcggcgca ccacggtgct ggcgatcggc tttccgtccg cggcctatcg cgcctcggcc
      481 gacctgctga tcgaccccga cgagttcgtc cgcaacgccc tcggcttcaa ggacgaggac
      541 gaggcgcccg gcgccggcgc gccccctgcc gacgtcgccc cggaaagcct gtcgctcgca
      601 gcgagcgcac gtttcgcgca ggcgtcgccg gaagcggccc ggagcgttcc cccggcctcg
      661 ctctccagcg gcgccgacgc cctcgaccac gacctcgccg agatcgtcga gaccatgcgc
      721 gtcgaggttg cgcgctcggc cgcgcccgtc ccctgcagcc gcctggcgag cctgatcacc
      781 acgcgccacc ccgccctcgc cgccgactgg aacggcaagg gcagcttccg caggttcgtc
      841 gacagcctcg acctcgcacc gctgcgtttc gactggagca acagcggcgg cttcgtcttc
      901 gatcccgccc gctccggcgc cggcatgcag gacggcaacg ccacgcccga ctggggcgag
      961 gaccaggaca tcctgccgct cgccatgcag atccacgagg tctccggcgt ccccctgctc
     1021 ggcccggccg aataccgcgc gctgttctcg atcatcgagg acgacgtcgg ccgccatccc
     1081 ttcgacctca aggaaaccgg caagcgcgtg cgcgaccgcc tgcgcgaagc cggtctcggc
     1141 ggcagccggc tcgacgtcaa ctggatcctg cgcggactgc tgatgcgcgg ccacgccttc
     1201 ggcgaggggc aggaccgcgc cgcgacgctc gccaccaaga cggcgaacaa cgtgcgctcg
     1261 ctctgcctgc gcgagcagat gatcctcgac cgcgcggccg agaccgcgat cgcgcgctgg
     1321 ttcggcaccc atccggctcc cacccgcagc gggcagtgag ccgatgcggg tgccgcggcc
     1381 ctcgactccg cctcccacac gcttctgagc tatattgcgt cgcaacagat ccagcacgag
     1441 gaccaccccc atggccagtc gcaacccctc cttcttccgc cgcatcggcc tcgccttctc
     1501 cgtcctcttc ggcgccgagc tcgccggccg cctcgtccgc ctgcgcgagg aaggcgcaga
     1561 caccgaggcc gctgccggca ccgaggacgc cgccgcagcc gcccagcccg ccatcgtgcg
     1621 cgagaccacc cccgaggccg cgctgcagct gctcggcctg ctccagcgcg acgcgcgctt
     1681 catcgatttc gtccaggaag acatcgccgc ctattcggat gccgacatcg gcgccgccgc
     1741 gcgcgtagtg cacgagggct gccgcaaggt gctcgacgag cacttcgcca tcgcgccggt
     1801 gcgcgacgag gccgaaggca gccgcctcac cctgcccgaa ggcttcgacg ccgcggccgt
     1861 gcgcctcacc ggcaaggtgg tgggcaagcc gcccttcacc ggcaccctcg gccatcgcgg
     1921 ctggcgcgtg accgacaccc gcctgccggc gctggccgaa ggccacgacg ccagcgtgat
     1981 cgcccaggcg gaggtggagc tgtgagcgcc cccgcccgct acaccctcgg catcgacctc
     2041 ggcaccaccc actgcgcgct ctcctatgtc gacctcgaag ccagcgacgg cgaccaggcc
     2101 gcgcagcagg tgctggagat cccccagctc accggcccgg gcagcatcga ggcgcgcccg
     2161 ctgctgccct ccttcctcta cctgccgcac gccgacgagc tcgcccccgg cgacctgagc
     2221 ctgccctggg gggcgacgca ggatcacgcg gtgggcgagt tcgctcgcag ccgcggggcg
     2281 gccacgccga tccgcctggt ggccagcgcc aagagctggc tgtgccaccc cggcgtcgac
     2341 cgccgcgccg acatcctgcc cgccgaggcg ccgcccgagg tggcgcgcat ctcgccgctg
     2401 accgcctcgg tgcgctacct gtcgcacctg cgcgcggcct ggaacgccgc ccatcccgag
     2461 gcgcccttcg acgcccagga cgtcaccgtc accatcccgg cctccttcga ccccgcggcg
     2521 cgcgagctca ccgccgaggc cgcgcaggcc gccggctacc cgccgatcac cctgctcgag
     2581 gagccgcagg cggcgctcta cagctggatc cagcgcagcg ccgggcagtg gcgcaaggaa
     2641 gtgaagaccg gcgacctgat cctggtggtc gacgtcggcg gcggcaccac cgacctgtcg
     2701 ctgatcgcgg tgctcgagcg cgagggcaac ctcgagctgc accgcgtggc ggtgggcgag
     2761 cacatcctgc tcggcggcga caacatggac ctggcgctgg cctacacggt cacgcgcaag
     2821 ctcgccgagc agggcacccg gctcgacccc tggcagacgc gcgcgctcgc ccatgcctgc
     2881 cgcggcgcca aggaggcgct gctgagcgac tccgacctcg acagcgtgcc ggtggtggtg
     2941 ccgagtcgcg gctcgaagct gatcggcggc tcgatccgca ccgaggtgcg gcgcgaggag
     3001 ctggtcgcca ccctggtcga gggcttcttc ccccgcgtcg cggtcaccga gatgcccatc
     3061 acgcgggcgc gcgccgcgct cacccagctc ggcctgccct atgcgcagga cgcggcggtc
     3121 acccgccacc tcgccgcctt cctcacccgc cagctcggcg ccaccgccga tctcgaaggc
     3181 ttcgccgcgc agcccgagga gagcgtcttc ctgcacccca ccgcggtgct gttcaacggc
     3241 ggcgtgctca agtccgggct gatcgccggg cgcgtgctcg acaccctgaa cgactggctc
     3301 gccgccgacg gcgccgcgcc ggcgcggctc ctcggcggcg ccgacctcga cctcgcggtg
     3361 gcgcgcggcg cggcctacta cggctacgtg cgccgcggac gcggggtgcg catccgcggc
     3421 ggcaccgcgc gcgcctacta cgtcggcatc gagtcctcga tgcccgccat cccgggcatg
     3481 gagccgccgc tcgaagccct ctgcgtcgcc cccttcggca tggaggaggg cagcgaggcc
     3541 gccctgcccg cgcaggagtt cggcctggtg gtcggcgagc cggtgcgctt ccgcttcttc
     3601 ggctcctcgg tgcgccgcca ggacgaggtc ggcaccctgc tcgacttctg gcagcccgac
     3661 gagctgcagg agctcgagga gatcgaggcc accctgccgg ccgaaggccg ccgccccggc
     3721 gaggtcgtgc gcgtgaagct gcacgcccgc gtcaccgaga ccggcacgct cgagctcgag
     3781 gccctgccgg tggacggcag cgcgcgctgg aaggtgcagt tcgacgtgcg cggtaacgca
     3841 gaagcctgag gtccttcgcg tggcgacgcg ctacaccgtc ggcatcgacc tcggcaccag
     3901 caacaccgtg gtcgccttcg ccgcagaggg cgaggaccgc atccaggtgc tggagatcga
     3961 gcagctcgtc ggccgcggcg agctcgccgc gcgcacgctg ctgccctcgg tgcgctacca
     4021 ccccgccgcg ggcgagctcg accccgccac gctgcgcctg gtcgcgagcg ccggcgaggg
     4081 gcagggcgcg ccgccggcgg tgctcggcga ggcggtggtc ggtcaatacg cccgcgagct
     4141 cggcgcccag gtgcccgggc gcgcggtggc cagcgccaag agctggctct cgcacgccgg
     4201 cgtcgaccgc aacgccgcga tcctgccctg gggcgcggcc gaggacgtgc cgcgggtgtc
     4261 gccggtggtg gcgagcgcga gctacctcgc ccacgtgcgc gcggcgtgga accgtcagca
     4321 tcccggggcc gcgctcgaaa atcaggacat cgtgctcacg gtgcccgcct ccttcgacga
     4381 gggtgcgcgc gccctcactc tggaggcggc gcggctagcc ggcctgccgc gcctgcgcct
     4441 gctcgaggag ccccaggccg ccttccacga ctggctgttc cgccaccgcg aacaactcgc
     4501 cgcggagctc gcgcagtccc gcctgctgct ggtgtgcgac atcggcggcg gcaccaccga
     4561 cctcaccctg atccaggtcg agcccgccgc ggacggtctc tcgccgccgc gcctgacccg
     4621 catcggcgtc ggcgaccacc tgatgctggg cggcgacaac atggacctgg cgctcgccca
     4681 cctggtggag aagcgcctgc tcgccggcgg cgagcgcctg tccgccgcac agctcgcaca
     4741 acttgtgcag cgctgccgcg cggcgaagga gcagctgctc gccgcggacg cgccggaaga
     4801 gctcaaggtc accctgctcg gcgccggatc gcgcctgatc ggtgcggcgc gctcgaccgc
     4861 gctacgccgc gacgaggtcc tgcgcctggt ggtggacggc ttcctgcctc tggtcggagc
     4921 cgacgaggca cccgcgcgcc ggcgcgccgc gatcgtcgag ttcggcctgc cctatcccgc
     4981 cgacccggcg atcagccgcc acctcgccgc cttcctgcgc cgccacggcc aggtttcgcg
     5041 cgtggcgctc gggatgcccg gtacgggcgc gggcagagat gccgacggag atgcggacga
     5101 agccgcagcc ccgctcgcga tccccgacac cctgctgctc aacggcggcg tgttccgcgc
     5161 cgacgcgatc tccgcccgcc tggaaggatt gctcgcacac tggcgcggcg cgcccccgcg
     5221 cgtgctgcac aacgacgccc ccgacctcgc cgtcgcccgc ggcgcggtgg ccttcgcgct
     5281 cgtgcgcgcg gcgcgcagcg gccccggcat cggcggcggc tcggcacgca gctacttcct
     5341 ggtgctggag gcgggtggcg aggccgcgcg cgcgatctgc gtgctgccgc gcggcaccga
     5401 atccggcacc gaggtcgcgc tccccgagcg tcgcttcgcg ctgcgcctcg gccagccggt
     5461 gcgcttccac ctcgtgtcct cgaccggcga ccgcgcgtgg caggcgggcg agctggtcga
     5521 gctcgacgcc gacgatttca tccacctgcc cccgctcgcc gcccgcctgc ccgccaccgc
     5581 cgccggacgc cgcgagaacg tcgccgtgcg cctggtcgcc tgcctgaccg aggtcggcac
     5641 gctggagatg cagtgcgtgg cgatcgacga cgacacgcaa cgctggcagc tcgccttcca
     5701 ggtgcgcgcc gaggccgaca tgcccgccac cgaggccgcg gccgcggccg gcgcccatcc
     5761 tcgcctcgcc caggccatcg ccctcatcga cgaggtcttc ggcgggcagg cgcgcgaact
     5821 cgacgccaag gcggtacgcc agctgcgcgc ccgcctcgag cgtgtgctcg gccgccgcga
     5881 cgactgggac atggcgctgc tgcgcgcgct cttcgacgcc ctgctcgagc gcgcgcgccg
     5941 gcgccggcgc tc
//
LOCUS       DAOQBS010000156         5926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_13584_length_5926_cov_14.615568, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000156 DAOQBS010000000
VERSION     DAOQBS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13584_length_5926_cov_14.615568"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKN25_13630"
     CDS             <1..741
                     /locus_tag="PKN25_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNV91987.1"
                     /translation="VYEGNWKLQAENGADGYHVTATHWNYAATQAQRKSRDAGDDIKA
                     MSAGGWAKKGGGSYSFENGHLLLWTRWDNPEDRPLMEQRDRLVKEFGEAKADWMIGNS
                     RNLCLYPNVYLMDQFSSQIRVFRPLDVNRTEVTIYCIAPKGESAEARARRIRQYEDFF
                     NASGMATPDDLEEFRACQEGFMGRALEWNDMSRGATHWVEGPDEEADKIDLKPILSGV
                     KTEDEGLYVAQHTYWLEEIRKAAAAKNA"
     gene            757..1245
                     /gene="benB"
                     /locus_tag="PKN25_13635"
     CDS             757..1245
                     /gene="benB"
                     /locus_tag="PKN25_13635"
                     /EC_number="1.14.12.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase small subunit"
                     /protein_id="HNV91988.1"
                     /translation="MSLSYENLCAFLYREARHLDDREWDEWLAMYDENCTFWMPAWDD
                     DGELTEDPQTEISLIWYGRRAGLEDRVFRIRTERSSATIPDTRTNHHISNIEVVEQND
                     KTVKLRFNWHTMSVRYNHTDHFCGTSFYTLDVSGASPLIKEKKVVLKTDYIRHVIDVY
                     HV"
     gene            1314..2324
                     /gene="benC"
                     /locus_tag="PKN25_13640"
     CDS             1314..2324
                     /gene="benC"
                     /locus_tag="PKN25_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase electron transfer
                     component BenC"
                     /protein_id="HNV91989.1"
                     /translation="MTFKIALNFEDGVTRFIDCNATETVADAAYRQGVNVPIDCRDGA
                     CGACKCKAESGKYELGFYIDDAMSEEEAKLGYVLTCQMHPKSDCVVNVPASSEVCKIK
                     HATLTATVAEVRQLSPSTLSLVLQGEGIGKLAFLPGQYANLTVPGTEQTRAYSFSSMP
                     ADSKVSFLIRNVPNGLMSGYLTGAAKPGDTIKLAGPIGSFYLRDVARPVLMLAGGTGL
                     APFLAMLDKLVAAGGSAHPVHLIYGVNTDADVVELQRLDAFKAALPNFTYDVCVVADD
                     SSWPKKGYVTAHIEPAHLNEGNVDIYLCGPPPMVEAVAHDLRERKVEPNSFHYEKFAA
                     SV"
     gene            2352..3128
                     /locus_tag="PKN25_13645"
     CDS             2352..3128
                     /locus_tag="PKN25_13645"
                     /EC_number="1.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate
                     dehydrogenase"
                     /protein_id="HNV91990.1"
                     /translation="MANRFKKKVAVITGAAQGIGKRVAEKMAKEKGTLVLVDRSELVH
                     EVAKELSGKVEVLSLTADLEKFADCKRVMEAAVEKFGRIDILINNVGGTIWTKPFEHY
                     DEDEIESEVRRSLFPTLWCCRAALPYMQEQGKGAIVNVSSIATRGVNRVPYGAAKGGV
                     NALTACLAFENAERGIRVNATAPGATEAPPRLIPRNTKEQSEQEKLWYKQIYTQSIDS
                     SLMKRYGTLDEQADPILFLASDDAAYITGTIVPVGGGDLG"
     gene            3435..4406
                     /locus_tag="PKN25_13650"
     CDS             3435..4406
                     /locus_tag="PKN25_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNV91991.1"
                     /translation="MPIRFLNGQSNVFAAANPFEVSEYVRANVGSHSLRLPRASDASA
                     SLSHRRAGTLDLCRLSYGAQARVLSESLGDIYHAQFILQGYCSYTLANRTLDLPAGHV
                     LVLNPDEPVDLTYSDNCEKFIVRIPSAMLDDACTEHRWFKPNERIKFSPEPQRFEDID
                     SLLLLLRLLCEEAESELATPQMLQHYCRVVTTKLMVMLKHNVSMVAPTRHAPSFERLV
                     NYIERNIKLDLSAEDLAHYAGLSLRSLYLLFEKNVKTTPKNFVRQKKLEKVHSILSDP
                     GQACPNVTAVALEYGFSHLGRFSELYKSTYGVLPSQSIRCRQPQPGR"
     gene            4513..4911
                     /locus_tag="PKN25_13655"
     CDS             4513..4911
                     /locus_tag="PKN25_13655"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNV91992.1"
                     /translation="MERIAINPPELFSGKAFSQTFSISTPQRLIFVSGQVDCDREGKV
                     RNPDDLEAQLKGTLDNLEIALRAQGASMRDLVQVTIYVVGLRPEDTTRIREIRSAYFD
                     AERPPAVTLLGIERLAFPGLRVEIDIIAAQ"
     gene            complement(4983..5549)
                     /locus_tag="PKN25_13660"
     CDS             complement(4983..5549)
                     /locus_tag="PKN25_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNV91993.1"
                     /translation="MNAPAAAPARITRIARRRARPGHEAEYEVMLREMLAKMREHKGF
                     LGGDLIPPEAAGEEYQLVIRFASEAELQAWDMSEARHELLERMKAVAEGEPEFRKLSG
                     LEAWFEPAVVPATMHPPRVRMAVVTWLGIFPTVSFFLWFVLPWIQPLPFLPRTAVLTA
                     LIVVTMTWVVMPRLTRLMRGFLNPPRKG"
     gene            complement(5774..>5926)
                     /locus_tag="PKN25_13665"
     CDS             complement(5774..>5926)
                     /locus_tag="PKN25_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNV91994.1"
                     /translation="GRKGEVFAIGPTVSYTTKGGVHFTGQWNRETGAENRFEGDKFIL
                     KLIMPL"
BASE COUNT         1142 a         2126 c         1787 g          871 t
ORIGIN      
        1 gtctatgaag gcaactggaa gctgcaggcc gagaacggcg ccgacggcta ccacgtcacc
       61 gccacgcact ggaactacgc cgccacccag gcgcagcgca agagccgcga cgccggcgac
      121 gacatcaagg ccatgagcgc cggcggctgg gccaagaagg gcggcggctc gtactcgttc
      181 gagaacggcc acctgctgct gtggacgcgc tgggacaacc ccgaagaccg tccgctgatg
      241 gaacagcgcg accgtctggt caaggaattc ggcgaagcca aggccgactg gatgatcggc
      301 aactcgcgca acctgtgcct gtacccgaac gtgtacctga tggaccagtt cagctcgcag
      361 atccgcgtgt tccgcccgct cgacgtcaac cgcaccgaag tcaccatcta ctgcatcgcg
      421 cccaagggcg agtcggccga ggcgcgtgcc cgccgcatcc gccagtacga ggacttcttc
      481 aacgcctcgg gcatggccac gccggacgac ctcgaagaat tccgcgcctg ccaggaaggc
      541 ttcatgggcc gtgcgctgga gtggaacgac atgtcgcgcg gcgccacgca ctgggtcgaa
      601 ggcccggacg aagaagcgga caagatcgac ctcaagccca tcctgtcggg cgtgaagacc
      661 gaggacgagg gcctctacgt cgcccagcac acctactggc ttgaagagat tcgcaaggct
      721 gccgcggcca agaacgcata accggagacc caagagatga gcctttccta cgagaacctg
      781 tgcgccttcc tgtaccgcga agcccgtcac ctcgacgacc gcgagtggga cgagtggctg
      841 gcgatgtacg acgagaactg caccttctgg atgcccgcct gggacgatga cggcgagctg
      901 accgaggacc cgcagaccga gatctcgctg atctggtacg gccgccgcgc cggcctggaa
      961 gaccgcgtgt tccgcatccg caccgagcgc tcgagcgcga ccatccccga cacccgcacg
     1021 aaccaccaca tcagcaacat cgaggtggtc gagcagaacg acaagacggt caagctgcgc
     1081 ttcaactggc acaccatgag cgtgcgctac aaccacaccg accacttctg cggcaccagc
     1141 ttctacaccc tggacgtgtc cggtgcgagc ccgctgatca aggaaaagaa ggtcgtcctg
     1201 aagaccgact acatccgcca cgtcatcgac gtttaccacg tctgacaacg agggcggggc
     1261 gtcgcgcgcc ggcaagggcg cgggcgaccc cgccccaacc gggagagaca accatgacat
     1321 tcaagatcgc gctgaacttc gaggacggcg tcacccgctt catcgactgc aacgccaccg
     1381 agaccgtggc cgatgcggcc taccgccagg gcgtgaacgt ccccatcgac tgccgcgacg
     1441 gcgcctgcgg cgcctgcaag tgcaaggccg aatcgggcaa gtacgagctc ggcttctaca
     1501 tcgacgacgc catgagcgag gaagaggcca agctcggcta cgtcctcacc tgccagatgc
     1561 accccaagag cgactgcgtg gtgaacgtgc ccgcctcgtc cgaggtgtgc aagatcaagc
     1621 acgccaccct caccgccacc gtggccgaag tgcgccagct ctcgccaagc acgctctccc
     1681 tggtgctgca gggcgaaggc atcggcaagc tcgccttcct gcccggccag tacgccaacc
     1741 tcaccgtgcc cggcaccgag cagacgcgcg cctactcgtt cagctccatg cccgccgaca
     1801 gcaaggtgag cttcctcatc cgcaacgtgc ccaacggcct gatgagcggc tacctcaccg
     1861 gcgccgcgaa gcccggcgac accatcaagc tcgccggccc gatcggcagc ttctacctgc
     1921 gcgacgtcgc ccgcccggtg ctgatgctcg ccggcggcac cggtctcgcg cccttcctcg
     1981 ccatgctcga caagctcgtc gcggccggtg gcagcgcgca cccggtgcac ctgatctacg
     2041 gcgtgaacac cgatgccgac gtggtcgagc tccagcgcct ggacgccttc aaggccgcac
     2101 tgcccaactt cacctacgac gtctgcgtgg tggccgacga cagcagctgg ccgaagaagg
     2161 gctatgtcac cgcccacatc gagcccgccc acctcaacga aggcaacgtg gacatctacc
     2221 tgtgcggccc cccgcccatg gtcgaggccg tcgcccacga cctgcgcgag cgcaaggtcg
     2281 aaccgaacag cttccattac gagaagttcg ccgccagcgt ctgagcgctg cccatcccgt
     2341 caggagcgac aatggcaaac cgtttcaaga agaaagtggc cgtcatcacc ggcgccgcgc
     2401 agggcatcgg caagcgcgtc gccgagaaga tggccaagga gaagggcacc ctggtgctgg
     2461 tcgaccgctc cgagctcgtg catgaggtgg ccaaggagct gtccggcaag gtcgaagtcc
     2521 tcagcctcac cgccgacctg gagaagttcg ccgactgcaa gcgggtcatg gaggccgcgg
     2581 tcgagaagtt cggccgcatc gacatcctga tcaacaacgt cggcggcacg atctggacca
     2641 agcccttcga gcactacgac gaggacgaga tcgagtccga ggtgcggcgc tcgctcttcc
     2701 ccaccctgtg gtgctgccgc gccgccctgc cctacatgca ggaacagggc aagggcgcga
     2761 tcgtcaacgt gtcctcgatc gccacccgcg gcgtcaaccg cgtgccctac ggcgcggcca
     2821 agggcggcgt caatgcgctc accgcctgcc tggcgttcga gaacgccgag cgcggcatcc
     2881 gcgtcaatgc caccgcgccc ggcgccaccg aggccccgcc gcgcctgatc ccgcgcaaca
     2941 ccaaggaaca gagcgagcag gagaagctct ggtacaagca gatctacacc cagtcgatcg
     3001 acagcagcct gatgaagcgc tacggcacgc tcgacgagca ggccgacccc atcctgttcc
     3061 tcgcctccga cgacgccgcc tacatcaccg gcaccatcgt gccggtgggc ggtggcgacc
     3121 tgggctgatt ttcccgcgtc ctctccctcc cctcggacgc ctcggccgcg ctgcagcgat
     3181 gcagcgcggc tttttttcgt ccccctgtct tgcggcctca gaattaaaaa actttatcca
     3241 tagaaccata gacagcgctt ttgcttccca ataaaatcga gattcttaag tttctcgaag
     3301 aatggccgat caagaagccg acttcgtccg acagagagcg agacagcctg cacgccaggc
     3361 ggagaggaga agcgcatgag caaggaacgc cggtttcaga cgcacgtgag caagcccgaa
     3421 tggaggaccg cgtgatgccc atccgcttcc tcaatggcca gagcaacgtc ttcgccgccg
     3481 ccaacccctt cgaggtctcc gagtacgtgc gcgccaacgt gggctcgcac agcctgcgcc
     3541 tgccgcgcgc cagcgacgcc agcgcctcgc tcagccaccg ccgcgccggc accctcgacc
     3601 tgtgccgtct cagctacggc gcgcaggccc gcgtgctgtc cgagagcctc ggcgacatct
     3661 accacgccca gttcatcctg caaggctatt gcagctacac gctcgccaac cgcacgctcg
     3721 acctgcccgc cggccacgtg ctggtgctca acccggacga gccggtggac ctcacctact
     3781 cggacaactg cgagaagttc atcgtccgca tcccctcggc gatgctcgac gacgcctgca
     3841 ccgagcaccg ctggttcaag cccaacgagc gcatcaagtt cagccccgag ccgcagcgtt
     3901 tcgaggacat cgacagcctg ctgctgttgc tgcgcctgct ctgcgaggag gccgaatccg
     3961 agctggcgac gccgcagatg ctgcagcact actgtcgcgt ggtcaccacc aagctgatgg
     4021 tgatgctcaa gcacaacgtc agcatggtcg cccccacccg gcacgcgccc agcttcgagc
     4081 gcctggtgaa ctacatcgag cgcaacatca agctcgatct cagcgccgag gatctcgccc
     4141 actacgccgg gctgagcctg cgctcgctct acctgctgtt cgagaagaac gtgaagacga
     4201 cgccgaagaa cttcgtgcgc cagaagaagc tcgagaaggt gcattcgatc ctgagcgacc
     4261 cgggccaggc ctgtccgaac gtcaccgcgg tcgcgctcga gtacggcttc tcgcacctgg
     4321 gccgcttctc cgaactttac aagtccacct acggcgtgct gccctcgcag tcgatccgct
     4381 gccgccagcc ccagcccggg cgctgaacag cgaccggcgc ccgcagccgg gcgccgcatc
     4441 ttccgtaccc cagcctgccc ccgccctcct ccgggcgggg cgcggtcacg accccacctg
     4501 gagaaatgcg ccatggaacg catcgcgatc aatccccccg agctcttcag cggcaaggcc
     4561 ttcagccaga cattctcgat cagcacgccg caacggctga tcttcgtgtc cggccaggtc
     4621 gactgcgacc gcgagggcaa ggtgcgcaac cccgacgacc tcgaggcgca actgaaaggc
     4681 acgctcgaca acctcgagat cgcactgcgc gcgcaaggcg cgagcatgcg cgacctggtg
     4741 caggtgacga tctacgtcgt cgggctgcgc cccgaggaca ccacgcgcat ccgcgagatc
     4801 cgctccgcct acttcgacgc cgagcgcccg ccggcggtga ccctgctcgg catcgagcgc
     4861 ctcgccttcc ccgggctgcg cgtcgagatc gacatcatcg cggcgcagtg acgcaaaggc
     4921 ccggcgcgcc gcgctccggc gccgccgggc aggcagggca cgcgcatgcc gtcggcgcgc
     4981 gatcagccct tgcgcggcgg attcaggaaa ccgcgcatca ggcgggtgag gcgcggcatc
     5041 accacccagg tcatcgtcac cacgatcagc gcagtgagca ccgcggtgcg cggcaggaag
     5101 ggcagcggct ggatccacgg cagcacgaac cacaggaaga aggacacggt ggggaagatc
     5161 cccagccagg tcaccaccgc catgcgcacg cgcggcggat gcatcgtcgc gggcaccacc
     5221 gcgggctcga accaggcttc gagcccgctc agcttgcgga attcaggctc gccctcggcc
     5281 accgccttca tgcgctcgag cagctcgtgt cgtgcctcgc tcatgtccca ggcctgcaac
     5341 tcggcctccg aggcgaagcg gatcacgagc tgatactcct cgcccgccgc ctcgggcgga
     5401 atcagatcgc cgcccagaaa acccttgtgc tcgcgcatct tggcgagcat ttcacgcagc
     5461 atcacttcgt actcggcctc atgccccggg cgcgcgcggc ggcgggcgat gcgggtgatg
     5521 cgggcgggag cggcggcagg tgcattcatc gaggactctc ctgttctgac ggggcggtga
     5581 agccggattg cgcatcaccg cgggtagagc tctactctaa cgcataattc tcgagatttt
     5641 tataaataat cgtgattaat attttgcatg gtcacgatcg gccgctccgc gcacacggca
     5701 gacgaaaaac cccggccacc aggacggtgt gccggggttg tgcgcgcagc tccgggaaag
     5761 gccccgccgc ctcttacagc ggcatgatca gcttgaggat gaacttgtcg ccctcgaagc
     5821 ggttctccgc gcccgtttca cggttccact ggccagtgaa atgcacgccg cccttggtcg
     5881 tgtagctgac ggtggggccg atggcgaaga cctcaccctt gcgtcc
//
LOCUS       DAOQBS010000157         5880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_13740_length_5880_cov_16.913476, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000157 DAOQBS010000000
VERSION     DAOQBS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13740_length_5880_cov_16.913476"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..788
                     /locus_tag="PKN25_13670"
     CDS             60..788
                     /locus_tag="PKN25_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV91995.1"
                     /translation="MQAPLESHVTLLVVDDDLVAQARMNAYFSREGYRVLLAGDGESF
                     WRQLAQGEVDLVLLDINLPGQDGLSLARELRARDASIGIILVTSRNDDVDKIVGLEVG
                     ADDYVTKPFNPRELLARVKSLLRRSATRPAETADSLRFAGWVLELSRRRLRDRAGQDV
                     ALTRGEFEVLALLVRHPGEILDRDRLSRAVSGHDWSPQDRSVDVLVRRLRAKLDDPAR
                     TDSLIATIRGEGYRLAADVEPGGV"
     gene            complement(749..3754)
                     /locus_tag="PKN25_13675"
     CDS             complement(749..3754)
                     /locus_tag="PKN25_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV91996.1"
                     /translation="MKPRHERRFGLRERLLLGLLVAALGTVLVAVVGWVSFQRVVSSQ
                     QAIVRDTLPAADALHEAVRANARLAALAPRLLRADSAAELDQLRAALGVDVPLVRDRL
                     AALRSPHVEAELRERLQATGDRLAAGLEGMSAAIDQRLRLRAARLERIDTLRAAIDGL
                     DELARIHADNATAQLVSTLTTLLQPDPGQGAPSLAEREAARERVLDLDIDSLERMHEL
                     NLTVHALGFLIGRLDELDSGARLQAARVEFAAHLALLARRLADIADPGGREQGERVLR
                     LLASALDEEGTFALRAREIALRAQVETLQGVVGELTTELDALAGELIHRGGRILAAAG
                     SAAERSATSGLIAFGVIAAALLLVTLGVTVHALRRHTLGRLRALEEATLALASGRREV
                     AIDTAGDDELASLAVALERFRANAIERDRFAEELRQQQQELENQVLARTAQLREANAA
                     LARETADHARARHAAEQADRAKTAFLGTVSHELRTPLAGILGLLELVEDASSTTERKQ
                     YQAQMRAAAVLLLELLEDMLDFARIEAGGVQVDKASFSLRDTVNDVFAVQGTRAAVRG
                     LALIAEVDPALPDAVQGDRRKLSQILLNLVGNAIKFSDAGAVTVRVRAGTQPDQLHFA
                     VEDHGIGIEPARQQEVFEPFVQVRDSGRHHAGTGLGLAVCRRLVEAMGGAIELKSAPG
                     QGTTVSFEIPLPATAAAAVEALPAATEPERTALPPGHRVLVVEDDEVNRMVVERFLDA
                     LGQQAVCAPDIQNALRLLQARPIDLALIDMNLPDGDGRELLARLRALPAHAHTPAVLM
                     SAHIPRREVDALLEAGFAAFLSKPFARERLRALLADLLAEPAAAAPTQGTAGVAEVLQ
                     ATDWIDADFLRAEREALGEETVADIVGVFRTQGQPLIDALLAAARAGEHEACARLAHK
                     LRGAAGNVGAGRLAECAGALEEALKPDPKGDAPRAGAIGDLAVRAGELGEAWSYTLQA
                     LDALRSAADDAEAQRPDQTPPGSTSAASR"
     gene            3860..4933
                     /gene="torT"
                     /locus_tag="PKN25_13680"
     CDS             3860..4933
                     /gene="torT"
                     /locus_tag="PKN25_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HNV91997.1"
                     /translation="MLGLALGALPSCAAALAGAQPAAEWPLQRWAGDHRAANGAAETG
                     PDIVPPSPTRGWRLCAIYPHLKDSYWLAVNFGMTEEARALGLGLGVREAGGYGQLARQ
                     RERVAECLEDAAVDALLIGTVSRSGLNDLLAPQLDHRLVLGVVNDIDPQVVRARIAVP
                     WYQLGWTIGRWLAARHPAGGPPARIAWIPGPADADWVGFIDRGFRTGIAGSAVNVVAE
                     RHGDTGRAIQRRLVEAVLDEQPQLDYLVGNAPMAEAAIAELRRRGREGATAIVSSYLT
                     PAVHGGILRGRILAAVTDFPVLQGRLAVRQALRALEGRPIEPYLGPAVELVDKSSLGR
                     FPVQWMLPPAGFVPVYQIAPALP"
     gene            4962..5792
                     /locus_tag="PKN25_13685"
     CDS             4962..5792
                     /locus_tag="PKN25_13685"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HNV91998.1"
                     /translation="MNPPADPLARIARWLREADGLIVAAGAGMGVDSGLPDFRGPEGF
                     WRAYPALGRAHVRFEEIANPAAFERDPALAWGFYGHRLALYRRTVPHRGFALLQEIAA
                     GLRGGAFVFTSNVDGQFQKAGFADARIMEVHGSIHHLQCQRGCRGAVWCAEGFEPQVD
                     EEACRLLGEPPRCPHCGGVARPNILMFGDWAWIPRRTEAQEAGLVAWLEHVRRPVVVE
                     LGAGENVPTVRRFAESIGGRLVRINPQAEPMLPAGAIHLRCGALEGIATVHAALVGHR
                     "
BASE COUNT          745 a         2086 c         2191 g          858 t
ORIGIN      
        1 atcccgcctg tcctcctcgc tccttcctgc ctcccacccc tcgcgtccct cgcgatccca
       61 tgcaagcgcc cctcgaatcc cacgtcaccc tcttggtcgt cgacgacgat ctcgtcgcgc
      121 aggcgcgcat gaatgcctac ttttcaaggg aaggctaccg cgtgctgctg gccggcgacg
      181 gcgagtcctt ctggcgacag ctcgcccagg gggaggtcga cctcgtgctg ctcgacatca
      241 acctgcccgg tcaggacggc ttgtcgttgg cgcgcgagct gcgcgcgcgc gacgcctcga
      301 tcggcatcat cctcgtcacc agccgcaacg acgacgtcga caagatcgtc gggctcgagg
      361 tgggcgccga cgactacgtg accaagccct tcaacccgcg cgagctgctc gcccgcgtca
      421 agagcctgct gcggcgcagc gcgacgcgtc cggcggagac ggcggacagc ctgcgcttcg
      481 cgggctgggt gctggaactt tcgcggcgca ggctgcgcga ccgcgcgggg caggacgtgg
      541 cgctgacgcg cggcgaattc gaagtgctcg cgctgctggt gcgccacccc ggcgagatcc
      601 tcgaccgcga tcgcctcagc cgcgcggtga gcgggcacga ctggtcgccg caggaccgtt
      661 cggtggatgt gctggtgcgc cgcctgcgcg cgaagctcga cgatccggcg cgtacggact
      721 cgctgatcgc aacgatccgc ggcgagggct atcggctggc tgccgacgtc gagcctggcg
      781 gggtctgatc cggcctttgc gcttcggcgt cgtccgccgc agaacgcaag gcgtcgagcg
      841 cctgcagcgt gtacgaccag gcctcgccca gctcgcccgc gcgcacggcc aggtcgccga
      901 tcgcgcccgc gcgcggcgcg tcccccttcg ggtccggttt cagcgcctcc tccagcgcgc
      961 ccgcgcattc cgccagccgc cctgccccca cgttgcccgc ggcgccgcgc agcttgtgcg
     1021 cgaggcgtgc gcaggcttcg tgctcgccgg cgcgggcggc ggcgagcagc gcgtcgatca
     1081 gcggctggcc ctgcgtgcga aagacgccga cgatgtcggc cacggtctcc tcgcccagcg
     1141 cctcccgctc cgcacgcagg aaatcggcgt cgatccagtc tgtcgcctgc aggacctcag
     1201 cgacacccgc cgtgccttgc gtgggcgctg cggccgctgg ctcggcaagg agatcggcga
     1261 gcagcgcgcg caggcgctcg cgcgcgaagg gcttggacag gaaggcggcg aagcccgctt
     1321 cgagcagcgc atcgacctct cggcgcggga tgtgggccga catcagcacg gccggagtgt
     1381 gcgcgtgcgc cggcagcgcg cgcaggcggg cgagcagctc gcggccgtcg ccgtcgggca
     1441 ggttcatgtc gatcagggcg aggtcgatcg ggcgcgcctg cagcaggcgc agtgcgttct
     1501 ggatgtcggg cgcacacacc gcctgctgac cgagggcgtc gaggaagcgc tcgaccacca
     1561 tgcgattgac ctcgtcgtcc tcgaccacca gcacgcggtg gccggggggc agcgcggtcc
     1621 gctccggctc cgtcgcggca ggcaaggcct cgacggccgc cgctgcggtc gccgggaggg
     1681 ggatctcgaa actcaccgtg gtgccctgcc ccggcgcgct cttcagctcg atcgcgccgc
     1741 ccatggcctc gaccaggcgg cggcacaccg ccagcccgag gccggtgccg gcgtggtggc
     1801 ggccgctgtc gcgcacctgc acgaagggct cgaagacctc ctgctggcgc gcgggctcga
     1861 tgccgatgcc gtggtcctcg acggcgaagt gtagctggtc gggttgcgta ccggcgcgca
     1921 cgcgcaccgt caccgcgccc gcgtcgctga atttgatcgc gttgccgacc aggttgagca
     1981 ggatctggct gagcttgcgg cggtcgccct gcaccgcgtc gggcagcgcg ggatcgacct
     2041 cggcgatcag cgcgagcccg cgtaccgcgg cgcgcgtgcc ctgcaccgcg aagacatcgt
     2101 tcaccgtgtc gcgcaggctg aagctcgcct tgtcgacctg cacgccgccc gcttcgatgc
     2161 gggcgaagtc gagcatgtcc tcgagcagct cgagcagcag cacggccgcg gcgcgcatct
     2221 gcgcctggta ctgcttgcgc tcggtggtcg agctggcgtc ctcgacgagt tcgagcagac
     2281 cgaggatacc cgcgagcggg gtgcgcagct cgtggctgac ggtgccgagg aaggcggtct
     2341 tggcgcggtc ggcctgctcg gcggcgtgac gggcgcgagc gtggtcggcg gtctcgcgcg
     2401 cgagcgcggc gttggcctcg cgcagctgcg cggtgcgcgc cagcacctgg ttctcgagct
     2461 cctgctgctg ctgccgaagc tcttctgcga agcggtcgcg ctcgatcgcg ttggcgcgga
     2521 agcgttcgag cgcgaccgcg agcgaggcca gctcgtcgtc gcccgcggtg tcgatcgcca
     2581 cctcgcgtcg gccggacgcg agtgcgaggg tggcctcttc cagcgcgcgc aggcgcccga
     2641 gcgtgtgacg ccgcagcgca tggacggtga cgcccagggt caccagcagc agcgccgcgg
     2701 cgatcacgcc gaaggcgatc agacccgagg tcgcgctgcg ttcggcggcc gatccggcag
     2761 cggcgaggat gcggccgccg cggtggatca gctcgccggc gagggcgtcg agctcggtgg
     2821 tgagttcgcc gaccacgccc tgcaaggtct ccacctgcgc gcgcagggcg atctcgcgtg
     2881 cgcgcagtgc gaacgtaccc tcctcgtcca gcgccgaggc gagcaatcgg agcacccgct
     2941 cgccctgctc ccgcccaccc gggtcggcga tatccgcgag ccggcgcgcc agcagtgcga
     3001 ggtgcgcggc gaactcgacg cgtgccgcct gcagccgtgc gcccgagtcg agctcgtcga
     3061 ggcggccgat gagaaagccg agcgcatgca cggtgaggtt gagttcgtgc atgcgttcga
     3121 ggctatcgat gtcgagatcg agcacacgct cgcgcgcagc ctcgcgctcg gccaggctcg
     3181 gcgcgccctg cccggggtcg ggctgcagca gggtcgtgag ggtggacacg agctgcgcgg
     3241 tggcgttatc cgcgtggatg cgggcaagct cgtcgagccc gtcgatcgcc gcgcgcagcg
     3301 tgtcgatgcg ctcgaggcgg gctgcacgca ggcgcaggcg ttggtcgatc gccgcgctca
     3361 tgccttcgag gccggcggcg aggcggtcgc cggtcgcctg caggcgctcg cgcagctccg
     3421 cctcgacgtg cggcgagcgc agcgccgcca ggcggtcgcg caccaacggc acgtcgacac
     3481 cgagcgcggc gcgcaactga tcgagctcgg ccgccgaatc cgcccgcagc agccgcggcg
     3541 caagcgccgc gaggcgggcg ttggcgcgca ccgcctcgtg cagcgcgtcg gcggccggca
     3601 gggtgtcgcg cacgatcgcc tgctggctgc tcaccacgcg ctggaagctg acccagccca
     3661 ccaccgccac cagtacggtg ccgagcgcgg cgacgagcag ccccagcagc aggcgttcgc
     3721 gcaggccgaa gcggcgttcg tggcggggtt tcacgtaaga tggggcggtc acggtcggtg
     3781 gggagcgggt gtgcttgcgc agtggtcgaa ggcgacgagt cgatgggttt catcttaccg
     3841 gcgcagcgtc cgccacggtt tgctcgggct tgcgctgggc gcgctgccga gctgcgccgc
     3901 ggcgctggcc ggtgcgcagc cggcagccga gtggccgctg caacgctggg caggcgatca
     3961 tcgcgcggca aacggcgcgg ccgagaccgg gccggacatc gttccgccct cccccacgcg
     4021 tggctggcgc ctgtgcgcga tctacccgca cctgaaggac agctactggc tggcggtgaa
     4081 tttcggcatg accgaggagg cgcgcgcgct cggcctcggc ctgggcgtac gcgaggccgg
     4141 cggctacggg cagctcgccc gccagcgcga acgcgtggcg gagtgcctgg aggatgcggc
     4201 ggtcgatgcg ctgctgatcg gcaccgtcag ccgcagcggg ctcaacgacc tgctcgcgcc
     4261 gcaactcgac catcggctcg tgctcggggt ggtgaacgac atcgacccgc aggtggtgcg
     4321 cgcgcgcatc gccgtgccct ggtaccagct cggctggacg atcggccgct ggctggccgc
     4381 acgccatccc gcgggcggtc cccccgcgcg gatcgcgtgg attcccggac cggcggacgc
     4441 ggactgggtg ggcttcatcg accgcggttt ccgcacgggc atcgcaggca gcgcggtgaa
     4501 cgtcgtcgcc gagcgccacg gtgacacggg gcgcgcgatc cagcgccggc tggtggaggc
     4561 tgtgctcgac gagcagccgc agctcgatta cctggtcggc aacgcgccca tggccgaggc
     4621 cgcgatcgcc gagctgcgcc ggcgcggacg cgagggggcg acggcgatcg tgtcgtccta
     4681 cctgactccg gccgtccatg gtggcatcct gcgcggccgc atcctcgcgg cggtgacgga
     4741 tttcccggtg ctgcaggggc ggctggcggt gcgtcaggcc ttgcgggcgc tggaggggcg
     4801 gccgatcgag ccctacctcg gcccggcggt cgaactcgtg gacaagagct cgctgggacg
     4861 tttcccggtg cagtggatgt tgccgccggc gggtttcgta ccggtgtatc agatcgcacc
     4921 ggcactgccc tgagaaatcc cttctccgcc tccgaaagcc gatgaatcca cctgccgatc
     4981 cccttgcccg catcgcccga tggctgcgcg aggccgacgg gctgatcgtc gccgcgggcg
     5041 cgggcatggg ggtggattcc gggctgccgg atttccgcgg ccccgagggc ttctggcggg
     5101 cctatccggc gctcggccgc gcccacgtgc gcttcgagga gatcgccaat ccggccgcgt
     5161 tcgagcgcga tccggcgctc gcctgggggt tctacgggca ccgcctggcg ctctaccggc
     5221 gcaccgtgcc gcaccggggc ttcgcgctgc tgcaggagat cgccgcggga ttgcgcggcg
     5281 gcgccttcgt ctttacctcg aatgtggatg ggcagtttca gaaggccggc tttgccgatg
     5341 cgcggatcat ggaggtgcac ggctcgattc atcacctgca gtgccagcgc ggctgccggg
     5401 gcgcggtgtg gtgcgcggag ggcttcgagc cgcaggtgga cgaggaagcc tgcagactgc
     5461 tcggcgagcc gccgcgctgc ccgcattgcg gcggcgtcgc gcggcccaac atcctgatgt
     5521 tcggcgactg ggcctggatt ccacgccgca ccgaggcgca ggaggcgggg ctggtcgcct
     5581 ggctggagca cgtgcgcagg cccgtggtgg tcgagctcgg cgcgggcgag aacgtgccca
     5641 cggtgcgccg cttcgccgag tcgatcggcg gacggctggt gcgcatcaac ccgcaggcgg
     5701 agccgatgct cccggcgggc gcgatccacc tgcgctgcgg tgcgctcgaa ggcattgcca
     5761 ccgtccatgc ggccctggtc gggcaccgct gacccgacgc ccgattcgcg ccttgcggcg
     5821 ctccttctac atgaaccggc acggctgcgg gcgtaaccgg cgatcagctg tcgacgtgcg
//
LOCUS       DAOQBS010000158         5634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_14651_length_5634_cov_18.148414, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000158 DAOQBS010000000
VERSION     DAOQBS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14651_length_5634_cov_18.148414"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="PKN25_13690"
     CDS             complement(<1..1310)
                     /locus_tag="PKN25_13690"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNV91999.1"
                     /translation="MSSALVWFRRDLRSFDHAALHHALRFAARVHCAFVFDTAILDAL
                     PARADRRVEFIHASLAELDAALDAMARAAGGTGGGLIVRHGRAEDEIPRLAAELGVDK
                     VFANRDYEPDAILRDSRVAQRLAEDGIGYEDFKDQVVFERDEVLTQTGKPYSVFTPYK
                     NAWLKAVDDFQVSSHAVEKYAAHLAPKPAGETLPGLADIGFAPTNLAELNMPVGMSGG
                     KRLFDDFAARIERYKRARDFPAVKGVSYLSTHLRFGTVSIRQLAAHARARGGEGAETW
                     LSELIWRDFYHQILWHHPQVVDRAFRPEYDAVQWDDAPELFAAWCEARTGYPIVDAAM
                     RQLDQTGWMHNRLRMIVASFLTKDLGVDWRLGERYFAIRLNDYDLAANNGGWQWAAST
                     GCDAQPWFRIFNPVTQSEKFDPEGRFIRRYLPELARVPDKFIHAP"
     assembly_gap    1330..1429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1571..2137
                     /locus_tag="PKN25_13695"
     CDS             1571..2137
                     /locus_tag="PKN25_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNV92000.1"
                     /translation="METPTANFTHHFLIAMPNMVDPHFARTLTYIAEHNEQGALGIIV
                     NRPIDMTLASLFERIEVPLEAEGFAAQPVYFGGPVQTDRGFVLHRPLGDWHSTLKVTD
                     EIGLTSSRDVLQAIGAGGEPKDVIVTLGYAGWAAGQLEQELLDNAWLTVPADLEIVFE
                     LPPEERLVAAMQKLGIDFTNLSDVAGHA"
     gene            2130..2636
                     /gene="ruvX"
                     /locus_tag="PKN25_13700"
     CDS             2130..2636
                     /gene="ruvX"
                     /locus_tag="PKN25_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNV92001.1"
                     /translation="MPEAATAAPSPADTRFPRPAPDTLPARGTVLGFDFGLARIGVAS
                     GELETGLASALTTVHAEANDARFAAIARLIAEWRPVALVVGIPTHLDGREHELTARCR
                     RFANQLHGRHALPVFTADERLSSVAAEAELAEAGRKDWRERKAALDAAAARILLQTFL
                     DTRRHERP"
     gene            2623..3126
                     /gene="pyrR"
                     /locus_tag="PKN25_13705"
     CDS             2623..3126
                     /gene="pyrR"
                     /locus_tag="PKN25_13705"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNV92002.1"
                     /translation="MKDLDAEALCRQLAEQIRPHVGPATALVGIYTGGLWLAERLHAE
                     LGIAQPLGAIDVSFYRDDYASKGLHASPKKTEIPFDVDGAHVILVDDVLYTGRTTRAA
                     LNELFDFGRPARVELAVLVDRGGRELPVAARYCAHTLSVPLAREQNLELQRDQAGHLG
                     LELTRRA"
     gene            3181..4140
                     /locus_tag="PKN25_13710"
     CDS             3181..4140
                     /locus_tag="PKN25_13710"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNV92003.1"
                     /translation="MRNPQLNQHGELQHLLTLDGLPRNILNAILDTAAPFTEVAEREV
                     KKLPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVVNLNINTSSAAKGESLLDTVD
                     NLCAMQADMFVVRHAASGAPMLIAQHLQNTGRDHIHVVNAGDGRHAHPTQGLLDMYTI
                     RHFKRDFTNLTVAIVGDVLHSRVARSQIAALTTLGVPEVRVIGPKTLLPTEVERLGVR
                     VFHDMREGLEGVDVVMMLRLQNERMNGALLPTPQEYYKIWGLTAEKLALARPDAIVMH
                     PGPMNRGVEIDSAVADGAQAVILPQVTFGIAVRMAVMSMLGSR"
     gene            4174..5451
                     /locus_tag="PKN25_13715"
     CDS             4174..5451
                     /locus_tag="PKN25_13715"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNV92004.1"
                     /translation="MNILISNGRVVDPANRTDAVQNVYVAGGKIVALGQAPDGFVAER
                     TIDAGGLVVAPGFIDLAARLREPGYEYRATLESEMEAAMAGGVTSLAIPPDTDPVLDE
                     PGLVEMLTYRAKKLNRAHIYPVGALTIGLQGERLSEMAELVEAGCVAFSQANVPLVDN
                     TVLMRALQYAATFGFRVWLQPLAPFLSQVGHAHDGEVATRLGLSGIPVAAETVALYTY
                     LELARITGARLHITRLSSAAGLALIDQARAEGMDVTCDVSINHVHLCDMDIGYFNPNC
                     HLVPPLRSQRDREALARGLAEGRIDALCSDHTPVDDDAKQTPFSESEPGATGLELLLP
                     LTLKWADRAGLALLDGLARITSDAAKIVGITKAGHLSVGARADVCVFDPATHVTITRE
                     GLRSQGKNTPFLGMELPGKVRYTLVEGQVMFEG"
BASE COUNT          893 a         2070 c         1812 g          759 t
ORIGIN      
        1 ggggcgtgga tgaacttgtc gggcacgcgc gcgagttcgg gcaggtagcg gcggatgaag
       61 cggccctcgg ggtcgaactt ctcggactga gtgaccgggt tgaagatgcg gaaccagggc
      121 tgggcgtcgc agccggtcga ggccgcccac tgccagccgc cgttgttggc ggcgaggtcg
      181 tagtcgttga gccggatggc gaagtaacgc tcgcccaggc gccagtcgac gccgaggtcc
      241 ttggtgagga aggaggcgac gatcatgcgc aggcggttgt gcatccagcc agtctggtcg
      301 agctggcgca tcgccgcatc gacgatcgga tagccggtgc gcgcctcgca ccaggcggcg
      361 aagagctcgg gcgcgtcgtc ccactgcacg gcgtcgtact cgggacggaa ggcacggtcc
      421 accacctgcg ggtggtgcca caggatctgg tggtagaagt cgcgccagat cagctccgac
      481 agccaggtct ccgccccctc gccgccgcgc gcgcgggcgt gggcggcgag ctggcggatc
      541 gacacggtgc cgaagcgcag gtgggtggac agatagctga cgcctttcac cgccgggaag
      601 tcgcgcgcgc gcttgtagcg ctcgatgcgc gcggcgaagt cgtcgaacag gcgcttgccg
      661 ccgctcatgc ccacaggcat gttcagctcg gcgaggttgg tgggcgcgaa gccgatgtcg
      721 gccagcccgg gcagggtctc gccggcgggc ttcggcgcca ggtgcgcggc gtacttctcc
      781 accgcatgcg agctgacctg gaaatcgtcc accgccttca gccaggcgtt cttgtaaggc
      841 gtgaacacgc tgtagggctt gcccgtctgg gtgagcacct cgtcacgctc gaagaccacc
      901 tggtccttga agtcctcgta accgatgccg tcctcggcga gtcgctgcgc gacccggctg
      961 tcgcgcagga tcgcgtcggg ttcgtagtcg cggttggcga agaccttgtc gacgccaagc
     1021 tcggcggcca ggcgcgggat ctcgtcctcg gcgcggccat ggcggacgat cagcccgcca
     1081 cccgtgccac ccgcggcacg cgccatggcg tcgagcgccg catccagctc ggccaggctg
     1141 gcgtggatga actcgacgcg gcggtcggcg cgcgcgggca gcgcgtcaag gatggcggta
     1201 tcgaagacga aggcgcaatg cacgcgcgcg gcgaagcgca gggcgtggtg gagcgcagcg
     1261 tgatcgaagc tgcgcaggtc gcggcggaac cagacgaggg cggaagacat ttttttgtcc
     1321 aggacaaagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng acgatgccgg
     1441 cgaccgatcg ctgcaatcgc gacttgcgtc gctcccacag aaaccacgcg cggcctgccc
     1501 gacggcggga ggacccgctc gcgcacgccc ccacgccaag aagaccggcg ttcggcgtag
     1561 aatgccgcgc atggaaacgc ccacggccaa cttcacgcac cacttcctga tcgccatgcc
     1621 caacatggtc gatccgcact tcgcgcgcac gctcacctac atcgccgaac acaacgaaca
     1681 gggcgcgctc ggcatcatcg tgaaccggcc gatcgacatg accctggcca gcctcttcga
     1741 gcgcatcgaa gtgccgctcg aggccgaggg cttcgccgcg cagccggtgt atttcggcgg
     1801 cccggtgcag accgaccgcg gcttcgtgct gcaccgcccg ctcggcgact ggcactccac
     1861 gctcaaggtc accgacgaga tcggcctcac cagcagccgc gacgtgctgc aggccatcgg
     1921 cgccggtggc gaacccaagg acgtcatcgt cacgctcggc tacgccggct gggctgccgg
     1981 ccagctcgag caggagctcc tcgacaacgc ctggctcacc gtccccgccg acctcgagat
     2041 cgtgttcgag ctgccgcccg aggaacgcct ggtcgccgcc atgcagaagc tcggcatcga
     2101 cttcaccaac ctgagcgatg tcgccggaca tgcctgaggc ggccacggcc gccccgtccc
     2161 ctgcggacac ccgcttcccc cggcccgccc cggacacgct ccccgcgcgt ggcaccgtgc
     2221 tcggtttcga cttcggcctc gcccgcatcg gcgtcgccag cggcgaactc gagaccggcc
     2281 tggccagcgc gctcaccacc gtgcacgccg aggcgaacga cgcacgcttc gccgccatcg
     2341 ccaggctcat cgccgaatgg cggccggtcg cgctggtggt cggcattccc acccatctgg
     2401 acggccgcga gcacgagctc accgcgcgct gccgccgctt cgccaaccag ctccacggcc
     2461 gccacgccct gccggtgttc accgccgacg agcgcctgtc ctcggtcgcc gccgaagccg
     2521 agctcgccga ggccggccgc aaggactggc gcgagcgcaa ggcggcgctc gacgccgccg
     2581 ccgcccgcat cctcctgcaa accttcctgg acacacgacg ccatgaaaga ccttgacgcc
     2641 gaagccctgt gccggcagct cgccgagcag atccgccccc acgtcggccc cgccaccgcg
     2701 ctcgtcggca tctacaccgg cggcctgtgg ctggccgagc gcctgcacgc cgagctgggc
     2761 atcgcccagc cgctgggcgc gatcgacgtc tccttctacc gcgacgacta cgccagcaag
     2821 ggtctgcacg ccagcccgaa gaagaccgag atccccttcg acgtcgacgg cgcgcacgtg
     2881 atcctggtcg acgacgtgct ctacaccggc cgcaccaccc gcgccgcgct caacgagctg
     2941 ttcgacttcg gccgcccggc ccgggtcgag ctcgccgtgc tggtcgaccg cggcgggcgc
     3001 gaactgccgg tcgccgcgcg ctactgcgcc cacaccctgt ccgtgccgct cgcgcgcgag
     3061 cagaacctcg agctgcagcg cgaccaggcc ggccacttgg gcctcgagct gacacgtcgc
     3121 gcctgagccg gagccgccct ccccttcccc ctgcccaccg ctgatttcga ggcacgcccg
     3181 atgcgaaacc cccagctcaa ccagcacggc gaactgcagc acctgctcac gctcgacggt
     3241 ctgccgcgca acatcctgaa cgccatcctc gacaccgccg cgcccttcac cgaggtggcc
     3301 gagcgcgagg tcaagaagct gccgctcttg cgcggcaaga gcgtgttcaa cctgttcttc
     3361 gagaactcca cacgcacccg caccaccttc gagatcgccg ccaagcgcct gtcggccgac
     3421 gtggtgaacc tcaacatcaa caccagctcg gccgccaagg gcgagagcct gctcgacacc
     3481 gtcgacaacc tgtgcgcgat gcaggccgac atgttcgtcg tgcgccacgc cgccagcggt
     3541 gcgccgatgc tgatcgccca gcacctgcag aacaccgggc gcgaccacat ccacgtggtc
     3601 aacgccggcg acggccgcca tgcgcacccg acgcagggcc tgctcgacat gtacaccatc
     3661 cgccacttca agcgcgactt caccaacctg accgtggcga tcgtcggcga cgtgctgcac
     3721 tcgcgcgtgg cgcgcagcca gatcgccgcg ctgaccacgc tcggcgtgcc cgaagtgcgc
     3781 gtgatcgggc cgaagaccct gctgcccacc gaggtcgagc gcctcggcgt gcgcgtgttc
     3841 cacgacatgc gcgagggact cgagggcgtg gacgtggtga tgatgctgcg cctgcagaac
     3901 gagcgcatga acggcgcgct gctgcccacg ccgcaggagt actacaagat ctggggcctc
     3961 accgccgaga agctcgcgct cgccaggccc gacgcgatcg tgatgcaccc cggcccgatg
     4021 aaccgcggcg tggagatcga ctccgcggtg gccgatggcg cgcaggcggt catcctgccc
     4081 caggtcacct tcggcatcgc cgtgcgcatg gcggtgatga gcatgctcgg cagccgctga
     4141 agccgcggac agcacaggaa caaggaccca cgcatgaaca tcctgatttc caacggccgc
     4201 gtcgtcgatc cggccaatcg caccgatgcg gtgcagaacg tctacgtcgc aggaggcaag
     4261 atcgtcgccc tcggccaggc gccggacggc ttcgtcgccg agcgcacgat cgacgccggc
     4321 ggtctcgtgg tcgcccccgg cttcatcgac ctcgccgcgc gcctgcgcga acccggctac
     4381 gagtaccgcg ccacgctcga atccgaaatg gaggcggcga tggccggcgg cgtcaccagc
     4441 ctggcgatcc cgcccgacac cgaccccgtg ctcgacgagc ccggcctggt cgagatgctg
     4501 acctatcgcg ccaagaagct gaaccgcgcc cacatctatc cggtcggcgc actcaccatc
     4561 ggccttcagg gcgagcgcct gtccgagatg gccgaactgg tcgaggccgg ctgcgtcgcc
     4621 ttctcgcagg ccaacgtgcc gctggtcgac aacaccgtgc tgatgcgcgc gctgcagtac
     4681 gccgccacct tcggcttccg cgtctggctg cagccgctgg cgcccttcct gtcgcaggtg
     4741 ggccacgccc acgacggcga ggtggcgacg cggctgggcc tgtcgggcat cccggtcgcc
     4801 gccgaaaccg tggcgctcta cacctacctc gagctcgcgc gcatcaccgg cgcccgcctg
     4861 cacatcaccc gcctgtcctc ggccgccggc ctcgcgctca tcgaccaggc gcgcgcagaa
     4921 ggcatggacg tgacctgcga cgtgtcgatc aaccatgtgc acctgtgcga catggacatc
     4981 ggctacttca accccaactg ccacctcgtc ccgccgctgc gcagccagcg cgaccgcgag
     5041 gcactcgccc ggggcctggc cgagggccgc atcgacgcgc tgtgctcgga ccacaccccg
     5101 gtggacgacg acgccaagca gacgccgttc tccgaatccg aacccggcgc caccggcctc
     5161 gagctgctgc tgccgctgac gctgaagtgg gccgaccgcg ccgggctggc gctgctggac
     5221 gggctggccc gcatcacctc ggacgcggcg aagatcgtcg gcatcaccaa ggccggccac
     5281 ctctcggtgg gcgcgcgcgc cgacgtgtgc gtgttcgacc ccgcgaccca cgtcaccatc
     5341 acccgcgagg gcctccggag ccagggcaag aacacgccct tcctcggcat ggagctgccg
     5401 ggcaaggtgc gctacacgct ggtcgagggg caggtgatgt tcgagggctg agcccggtcg
     5461 ggcaaacgcc cgccggcccg gctcgcaaaa aggggcagcc gctaccggcc gccccttttt
     5521 gcgcttgaag ccggtgcgga aatcagcccc cgaacagcag cggcgcgaaa ccgttgcgcg
     5581 cgccggcgag caccatcagc acgatctgca gcaccagcag cagcaccagc ggcg
//
LOCUS       DAOQBS010000159         5634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_14652_length_5634_cov_15.361893, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000159 DAOQBS010000000
VERSION     DAOQBS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14652_length_5634_cov_15.361893"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="PKN25_13720"
     CDS             <1..156
                     /locus_tag="PKN25_13720"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376424.1"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer of AccA2D2 subunits; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HNV92005.1"
                     /translation="VREKLPEGFQRAEFLLEKGAIDLIVDRRELRDRLAVLLAMLNRQ
                     PADVVVG"
     gene            156..1469
                     /gene="folC"
                     /locus_tag="PKN25_13725"
     CDS             156..1469
                     /gene="folC"
                     /locus_tag="PKN25_13725"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HNV92006.1"
                     /translation="MAEQAASPASLDGWLALLEQRHGQRVELGLERVAAVRARMQAES
                     DAVVITVGGTNGKGSCCAMLEGILLASGYRVGCYTSPHLLRYNERVRIDGRDADDAAL
                     VAGFAAVEAARGDTALTYFEHGTLAAWQVFAAAGLDVIILEVGLGGRLDAVNVFEPDC
                     ALVTSVAMDHMDYLGDTREAIGFEKAGIFRAGRPAVCGDPQPPATLVEHAAAIGAQLW
                     ISGRDFGFGGDRQQWGYWRYAQPAREGALVRRGGLAYPALRGANQLLNAAAVITVLEC
                     LRERIPVSMQHVRQGLMLVELPGRFQVQPGRPSVVLDVAHNPQAAGVLADNLGNMGFY
                     PETWAVLGMLADKDVAGVVGLMAGRVDHWLLADLPGPRGLSAEALAAQVRAVKEGADI
                     RCFATPAEAYAAAQKLVAEDDRIVVFGSFLTVSDVLAAIKAARRG"
     gene            1479..2171
                     /locus_tag="PKN25_13730"
     CDS             1479..2171
                     /locus_tag="PKN25_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNV92007.1"
                     /translation="MTEADSLEIKKRARRRLVGAIALALLAAIVLPMMMEEEPNPSIE
                     DIRVSIPDRDKAASPRRGGAPENASAPVAVAVPEDDEVVAAEKPATEKAPAAGDKPAP
                     AASSAPAKPATPPTQPAAAMPQRPPAPVATPEPRRPSADEEARLRALIEGKASPARGD
                     SYVLQIGAFSDAVKAARLVDDLRARGFPAYTERAGNVMRVRVGPVAGRAEADATAARL
                     KAAGHPAVLQPR"
     gene            2183..2674
                     /locus_tag="PKN25_13735"
     CDS             2183..2674
                     /locus_tag="PKN25_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNV92008.1"
                     /translation="MTVFDYVFLGLLAFSALIGMWRGLVSEVMAVLAWAAALFAAWRY
                     NEEAAGVFTGMIVEPMWRQVAGGALVVVGVLILAALLRYLLRELLRAAGLGATDRFFG
                     ALFGIARGLAVAFVVVLIGGLAGVSKEPWWRDALFAAPLESAVIAAKPWLPAEVADKI
                     RFR"
     gene            2685..4220
                     /gene="purF"
                     /locus_tag="PKN25_13740"
     CDS             2685..4220
                     /gene="purF"
                     /locus_tag="PKN25_13740"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNV92009.1"
                     /translation="MCGILGVVATSPVNQLLYDGLQVLQHRGQDAAGIATSEGGRFHM
                     HKGSGLVRDVFRTRNMRNLQGNWGIGHVRYPTAGSAYNAAEAQPFYVNSPFGLLLAHN
                     GNLTNSEALKREMFLSDLRHINTSSDSEVLLNVFAHELQAACKGLKIDEEAIFTAVAG
                     VHRRCRGAYAVVVMIAGYGLLAFRDPYGIRPLVIGRNDCEAGTEWLVASESVAVDVLG
                     FRLLRDVAPGEAVLVDTAGRFHSRQCAARTVHAPCMFEFVYLARPDSIIDGVSVYESR
                     MKMGEFLADKMRRTMPNVHIDVVIPIPDSSRPSAMQMADRLDLPFREGFVKNRYIGRT
                     FIMPGQATRRKSVRQKLNTIHQEFKGKSVLLVDDSIVRGTTSKEIVNMAREAGATKVY
                     LASAAPPVRYANVYGIDMPTRGELIASDRSEEEICREIGADGLIYQDLDDLKASVRAL
                     NPSLQFFETSCFDGHYITGDITVEYLNGVENQRSEAKPGKNDQDDGDDGQLDLNLASD
                     AEH"
     gene            4254..5438
                     /locus_tag="PKN25_13745"
     CDS             4254..5438
                     /locus_tag="PKN25_13745"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HNV92010.1"
                     /translation="MNRPLPPELHPDTLAVRAGTERTQFREHGEAMYLTSSFVFDSAA
                     QAAACFSGEEEGYVYARFSNPTVTAMQNRLAALEGGEACIATASGMSAILSLAMATMQ
                     AGDHVVASNGLFGATQQLFGGILSKFGITTSFVPATELDAWRAAITPRTRLFFTETPS
                     NPLTEVIDIAGVAAIARAAGVIFAVDNCFCTPALQRPLELGADVVVHSATKYLDGQGR
                     VLGGAVVGSKAITDEVFKFLRTAGPTLSPFNAWVILKGLETLRIRMEAQSASALELAR
                     WLEAQPGVARVYYPGLESHPQHALAMRQQKSGGAIVSFDVKGGREAAWKVVDATRLIS
                     ITANLGDTKSTITHPATTTHGRISAEARATAGIGDGLLRIAVGLEDVDDLKADLARGL
                     AG"
BASE COUNT          826 a         2045 c         1920 g          843 t
ORIGIN      
        1 gtgcgcgaga agctgcccga aggcttccag cgcgccgagt tcctgctcga gaagggcgcg
       61 atcgacctga tcgtcgaccg tcgcgagctg cgcgaccgtc tcgcggtgct gctcgccatg
      121 ctcaaccgcc agcccgcgga cgtggtcgtc gggtgatggc ggagcaagcc gcatcgcccg
      181 ccagcctgga cggctggctc gccctgctgg agcagcgcca cggccagcgc gtcgagctcg
      241 ggctcgagcg cgtcgccgcg gtgcgtgcgc gcatgcaggc cgagtcggac gccgtggtga
      301 tcaccgtcgg cggcaccaac ggcaagggct cgtgctgcgc catgctcgaa ggcatcctgc
      361 tcgcctcggg ctaccgcgtg ggctgctaca cctcgcccca cctgctgcgc tacaacgagc
      421 gcgtgcgcat cgacggcagg gacgccgacg acgcggccct ggtggccgga ttcgccgccg
      481 tcgaggccgc ccgcggcgac accgcgctga cctatttcga gcacggcacc ctggcggcct
      541 ggcaggtctt cgccgcggca gggctggacg tgatcatcct cgaggtcggc ctcggcggcc
      601 ggctcgacgc ggtcaacgtg ttcgagcccg actgcgcgct ggtgaccagc gtggccatgg
      661 accacatgga ctacctcggc gacacgcgcg aggcgatcgg cttcgagaag gcgggcattt
      721 tccgcgccgg ccgtccggcc gtgtgcggcg atccgcagcc gcccgcgacc ctggtcgagc
      781 acgccgcggc gatcggcgcg cagctgtgga tcagcgggcg cgacttcggc tttggcggcg
      841 accgtcagca gtggggctac tggcgctacg cccagcctgc gcgcgagggt gcgctggtgc
      901 gccgtggcgg gctcgcctat ccggcgctgc gcggcgccaa ccagctgctc aacgccgcgg
      961 cggtgatcac cgtgctcgag tgcctgcgcg agcgcatccc ggtgtcgatg cagcacgtgc
     1021 gccagggcct catgctggtc gagctgcctg gccgcttcca ggtgcagccg gggcgcccct
     1081 cggtggtgct cgacgtcgcg cacaacccgc aggccgccgg cgtgctcgcc gacaacctcg
     1141 gcaacatggg cttctacccc gagacctggg cggtgctggg catgctcgcc gacaaggacg
     1201 tggcgggcgt ggtggggctc atggccggcc gcgtcgacca ctggctgctc gccgacctcc
     1261 ccgggccgcg tggcctttcc gccgaagcgc tcgccgcgca ggtgcgtgcg gtgaaggagg
     1321 gcgccgacat ccgctgcttc gccacgccgg ccgaggccta tgctgcggcg caaaagttgg
     1381 tcgccgagga tgatagaatc gtcgttttcg gttctttcct gacggtttcg gatgtgctcg
     1441 ccgcgatcaa ggcggcacgg cgcggatgag gtttggcggt gactgaggca gattccctcg
     1501 agatcaagaa gcgcgcgcgc cgtcggctgg tcggcgcgat cgcgcttgcc ctgctggccg
     1561 cgatcgtgct gccgatgatg atggaagagg agccgaaccc ctcgatcgag gacatccggg
     1621 tctcgatccc cgatcgcgac aaggcggcca gtccgcgccg cggcggcgcg cccgagaatg
     1681 cgagcgcgcc ggtcgccgtc gcggtgccgg aggacgacga ggtggtcgcg gccgagaagc
     1741 ctgcgaccga gaaggcaccg gcggcggggg acaagcccgc gcctgccgct tcctccgcgc
     1801 ccgcaaagcc cgccacgcca cccacgcagc ccgccgcggc gatgccgcag cgcccgcctg
     1861 cgccggtggc gacccccgag ccgcgccgcc cctccgccga cgaagaggcc cgcctgcgcg
     1921 cgctcatcga gggcaaggcc tcgcccgcgc gtggagacag ctacgtgctg cagatcggtg
     1981 ccttcagcga cgccgtcaag gccgccaggc tggtcgatga tctgcgcgcg cgcggcttcc
     2041 cggcctacac cgagcgcgcc ggcaacgtga tgcgggtgcg tgtcggtccg gtggccggtc
     2101 gcgccgaggc ggacgccacc gcggcccgcc tcaaggccgc cggacacccg gcggtcctgc
     2161 agccgcgctg acttcccgga tcatgaccgt cttcgactac gtctttctcg gcctgctcgc
     2221 cttctccgcg ctcatcggca tgtggcgcgg cctggtcagc gaggtcatgg cggtgctcgc
     2281 gtgggcggca gcgctcttcg ccgcgtggcg ctataacgaa gaagcggccg gggtcttcac
     2341 cggcatgatc gtcgagccga tgtggcgcca ggtcgcgggt ggtgcgctgg tcgtggtcgg
     2401 ggtgctgatc ctcgccgcgt tgctgcgtta cctgttgcgc gagttgctgc gcgccgccgg
     2461 gctgggcgcc accgaccgct tcttcggcgc gctcttcggc atcgcgcgcg ggctggcggt
     2521 cgccttcgtg gtggtgctga tcggcgggct cgccggcgtt tccaaggagc cgtggtggcg
     2581 cgacgcgctg ttcgcggcgc cgctggagtc ggcggtgatc gccgccaaac cctggctgcc
     2641 tgcagaggta gccgacaaga ttcgattcag ataggcctca atccatgtgc ggaattctcg
     2701 gtgtcgtcgc cacctccccg gtcaaccaac tgctctatga cggcctccag gtgttgcagc
     2761 accgcggcca ggacgcggcg ggcatcgcca cctcggaggg cgggcgtttc cacatgcaca
     2821 agggctccgg cctcgtgcgc gacgtgttcc gcacgcgcaa catgcgcaat ctccagggca
     2881 actggggtat cggccatgtg cgctacccga ccgcgggctc ggcctacaac gccgccgagg
     2941 cgcagccctt ctacgtcaac tcgcccttcg gcctgttgct ggcgcacaac ggcaacctga
     3001 ccaactcgga agcgctcaag cgcgagatgt tcctgtccga cctgcgccac atcaacacca
     3061 gcagcgactc cgaggtgctg ctcaacgtct tcgcacacga gctgcaggcc gcctgcaagg
     3121 ggctcaagat cgacgaggag gccatcttca ccgcggtcgc cggcgtgcat cggcgctgcc
     3181 gcggcgccta cgcggtggtg gtgatgatcg ccggctacgg tctgctcgcc ttccgcgacc
     3241 cttacggcat ccgccccctg gtgatcggcc gcaacgactg cgaggccggc accgaatggc
     3301 tggtggcctc ggaatcggtg gcggtggacg tgctcggctt ccgcctgctg cgcgacgtcg
     3361 cgccgggcga ggcggtgctg gtcgataccg ccggccgctt ccacagccgc cagtgcgccg
     3421 cgcgcacggt gcatgcgccg tgcatgttcg agttcgtcta cctggcacgg ccggattcga
     3481 tcatcgatgg cgtgtcggtg tacgagtcgc gcatgaagat gggcgagttc ctcgccgaca
     3541 agatgcgccg caccatgccc aacgtgcaca tcgacgtggt gatcccgatc cccgactcca
     3601 gccgcccctc ggcgatgcag atggccgacc ggctcgacct gcccttccgc gagggcttcg
     3661 tcaagaaccg ctacatcggc cgcaccttca tcatgcccgg ccaggccacg cgccggaagt
     3721 cggtacgcca gaagctcaac accatccacc aggagttcaa gggcaagagc gtgctgctgg
     3781 tcgacgactc catcgtgcgc ggcacgacca gcaaggagat cgtcaacatg gcgcgcgagg
     3841 cgggggccac caaggtctat ctcgcctcgg ccgcgccgcc ggtgcgctac gccaacgtct
     3901 acggcatcga catgcccacg cgcggcgagc tgatcgcctc cgaccgcagt gaagaggaga
     3961 tctgccgcga gatcggcgcc gacggcctca tctaccagga cctcgacgac ctcaaggcct
     4021 cggtgcgtgc gctcaatccc tcgctgcagt tcttcgagac ctcgtgtttc gacggccact
     4081 acatcaccgg cgacatcacc gtcgagtacc tcaacggcgt cgagaaccag cgcagcgaag
     4141 ccaagccggg caagaacgac caggatgacg gggacgatgg ccagctcgac ctgaacctcg
     4201 cctccgacgc cgagcactga gccgccccca agacgaaacc aagcacgacc acgatgaacc
     4261 gcccccttcc gcccgagctt caccccgaca ccctggccgt gcgcgccggc accgagcgca
     4321 cgcagttccg cgagcacggc gaggcgatgt acctcacctc gagcttcgtc ttcgacagcg
     4381 ccgcgcaggc cgccgcctgc ttctcgggcg aggaggaggg ctacgtctac gcgcgcttct
     4441 ccaaccccac cgtgaccgcg atgcagaacc ggctcgccgc gctcgaaggc ggcgaggcct
     4501 gcatcgccac cgcgtccggc atgtccgcga tcctgtcgct ggcgatggcg accatgcagg
     4561 cgggcgacca cgtggtggcc tccaacgggc tgttcggcgc cacccagcag ctcttcggcg
     4621 gcatcctgtc gaagttcggc atcaccacca gcttcgtgcc ggccaccgag ctcgacgcct
     4681 ggcgcgccgc gatcacgccg cgcaccaggc tcttcttcac cgagacgccc tccaacccgc
     4741 tcaccgaggt gatcgacatt gccggcgtgg cggcgatcgc gcgcgcggcg ggggtgatct
     4801 tcgcggtcga caactgcttc tgcacgccgg cgctgcagcg cccgctggag ctcggcgccg
     4861 acgtcgtcgt gcattcggcc accaagtatc tcgacggcca gggcagggtg ctcggcggcg
     4921 cggtggtcgg cagcaaggcg atcaccgacg aagtgttcaa gttcctgcgc accgccgggc
     4981 cgaccctgtc gccgttcaac gcctgggtga tcctcaaggg cctggagacg ctgcgcatcc
     5041 gcatggaggc gcagtcggcg agtgcgctcg agctggcgcg ctggctcgag gcccagccgg
     5101 gcgtggcgcg ggtgtactac cccgggctgg aatcccaccc ccagcacgcg ctggccatgc
     5161 gccagcaaaa gagcggcggc gccatcgtca gcttcgacgt gaagggcggc cgcgaggcgg
     5221 cgtggaaggt ggtcgacgcc acccgcctga tctcgatcac cgccaatctt ggcgacacca
     5281 agagcaccat cacccacccc gcgaccacca cccacggccg catcagcgcc gaggcgcgcg
     5341 ccaccgccgg catcggcgac ggcctgctgc gcatcgccgt cggtctggaa gacgtcgacg
     5401 acctcaaggc cgacctcgcc cgcggcctgg cgggctgacg cgcgtccgtc gcagatatca
     5461 caccggcctc tgcccgaagc gttcgagcct cctgcaggcg cgacgcaagt cgtatgggtt
     5521 cctgtaggag cgacgcaagt cgcgatgcgc ggcgccgatg cggatcgccg tcatttcagc
     5581 gcccgatcgc gacttgcgtc gctcccacgg aaaacccgcc acagcccggg cctg
//
LOCUS       DAOQBS010000160         5465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_15335_length_5465_cov_13.407579, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000160 DAOQBS010000000
VERSION     DAOQBS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15335_length_5465_cov_13.407579"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKN25_13750"
     CDS             <1..402
                     /locus_tag="PKN25_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNV92011.1"
                     /translation="AGVVRLEVDAAAADALERGLSGLVAEGLKLAVVRADAGAAAQAR
                     LARLELVGHDRPGIVRDISAVLARHGLSIDALETACESASMSGEPLFRARAELAVPAG
                     VGLAAVRNDLEALANELMVDLDFEEVSEPAG"
     gene            607..1374
                     /locus_tag="PKN25_13755"
     CDS             607..1374
                     /locus_tag="PKN25_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV92012.1"
                     /translation="MQIENGVFVITGGGSGLGAASARMLVEAGGRVVLADVNAQAGEA
                     LAAELGAAACFVQTDVTDADSAKAAIDTAVQRFGGLNGLVSCAGVAPAEKVVGREGPH
                     RLESFARTVSINLIGTFNMLRLAADVMSKAEPNAGGERGVIVNTASVAAFDGQVGQAG
                     YASSKAGVVGLTLPVARELSRFGIRVMAIAPGIMETPMLMGMPQEVQDSLGKMVPFPS
                     RLGKPAEFAALVRHIVENAYLNGEVIRLDGAIRMAAK"
     gene            1512..4031
                     /locus_tag="PKN25_13760"
     CDS             1512..4031
                     /locus_tag="PKN25_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNV92013.1"
                     /translation="MTPVEQPVSAAAAAADAAADRDCYHCGLPIPPETRHYVRIDGRE
                     RRMCCVGCEAVAQSIVDNGLVDYYRHRDAMPETRREAMPVELQELGLFDHPDFQKSFV
                     RPVGEHEREAALILEGITCAACVWLNENHVARLSGVSAIEINYATRRARVRWDERRIK
                     LSDILAAIQAIGYRAYPYDAERSEQLAHRERRSMLWRVFVAGFGMMQVMMYAFPVYVA
                     GEGDMTWDIEQLLRWASLLLTLPVVLYSAAPFFQRAWRDLRLRRLGMDVPVALGVGSA
                     FAASLWATLTDGPEVYFDSVTMFVFFLLGGRYLEMIARQKAVRGVEELGKALPSFAER
                     IAGWPGESAGERVPTSQLVAGDVLRVRPGEVIPVDGLVVEGRGEANEALLTGESMPVP
                     KAVGDRVTGGSINVSSPLLVRVEQVGDATRLAAIRRLMERAATEKPRIASLSDRIAAY
                     FIVTLLVLAAGTGVAWYLIDPARALWVFVSVLVVACPCALSLATPTALTVATDTLARM
                     GVLVTRGHAIETLARANRFVFDKTGTLTLGKMRVEEVCALGSVDADRLVVLAAAIEQG
                     SAHPVAAGLRAAAGEAALPAVAELAAETGQGMHGVVEGERLWIGRPGWVAGVAGLAAP
                     AALAGFAAPGGTVIALAGRGGWLGLFRLGDSLRADAPMITRRLAAEGAALGLLSGDAQ
                     PVVDAVAARLGIHDARGGLDPQGKQQAIVDMQRDDRAVVAMIGDGVNDAPVLAQAHVS
                     VAMGGGTDLARNQADIVLLNERFVSLADGIVLARRTLRIIRQNLWWSFAYNFTSVPLA
                     MAGLVTPWMAGIGMAASSLLVVLNAMRLQRATRTERAGAEN"
     gene            4033..4287
                     /gene="ccoS"
                     /locus_tag="PKN25_13765"
     CDS             4033..4287
                     /gene="ccoS"
                     /locus_tag="PKN25_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNV92014.1"
                     /translation="MESLYLLIPLSVVLVFVIGVLFWWSLRSGQYDDLEGPAYRLLMD
                     DREQLPGDGQERDADPSGEVAANGVSDRRTGSAGEQSRFR"
     gene            4703..>5465
                     /locus_tag="PKN25_13770"
     CDS             4703..>5465
                     /locus_tag="PKN25_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNV92015.1"
                     /translation="MQSQATYNYKVVRQFAIMTVVWGIVGMLVGVIAAAQLVWPQLNV
                     HEFLHFGRLRPLHTNAVIFAFGGCALFATSYYVVQRTCHTRLFAPGLAAFTFWGWQLV
                     ILLAAITLPLGYTTSKEYAELEWPIDILIAIVWVSYAIVFFGTIARRKVSHIYVANWF
                     FGGFILTVALLHIVNSAAIPVSLTSMKSYSAYAGVQDAMIQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQADRPVYSYRLSVVHFWALIFTYMWAG"
BASE COUNT          750 a         1763 c         1937 g         1015 t
ORIGIN      
        1 gccggcgtcg tgcgcctcga agtggatgcc gcggcggccg acgcgctcga gcgcggcctg
       61 agcgggctgg ttgcggaagg gctcaagctg gccgtcgtgc gggccgatgc gggcgccgct
      121 gcgcaggcgc gcctggcgcg tctcgagctc gtcggccacg atcgtcccgg catcgtgcgc
      181 gacatttcgg ccgtgctcgc tcgccacggc ctcagcatcg atgcgctgga gacggcttgc
      241 gagagtgcct cgatgagcgg cgagccgctc tttcgcgcgc gcgcggagct ggcggtgccg
      301 gcaggggtcg gcctcgctgc ggtgcgcaac gacctggagg cgctcgcgaa cgagctgatg
      361 gtcgatctcg atttcgagga agtgtccgag ccggccggct gaggactcgc ggagttcgcg
      421 ggggcgtggc gcgtgccgcg catccgctcg acatgccggg gctccgcggt gttcgcgtgc
      481 gcttgcgcgc gcgccacgtt ggggcagcga cgtggcgatc cgattgacgc ctacgtcaac
      541 gtaatttaag gtgctgcgct gcagcggacg gcgtccgccg tcaccgattt ttcgaggaga
      601 aaggcgatgc agatcgagaa tggcgtattc gtgatcacgg gtggtggctc gggcctgggc
      661 gcggcgagcg cgcgcatgct ggtcgaagcc ggtggccgcg tggtgctggc cgacgtcaac
      721 gcgcaggcgg gcgaggcgct cgccgccgaa ctcggcgctg cggcctgctt cgtgcagacc
      781 gacgtcaccg atgccgacag cgccaaggcg gcgatcgata ctgcggtgca gcgcttcgga
      841 ggcctcaacg gcctggtcag ctgcgccggt gtggcgccag ccgaaaaggt ggtgggccgc
      901 gaggggccgc atcggctcga gagcttcgca cgtaccgttt cgatcaacct gatcggcacc
      961 ttcaacatgt tgcgcctcgc cgccgacgtc atgagcaagg ccgagcccaa tgccggcggc
     1021 gagcgtggcg tgatcgtgaa taccgcgtcg gtggccgcct tcgacggcca ggtcggacag
     1081 gccggctacg cctcgtccaa ggctggcgtg gtcggactca cgctgccggt ggcgcgcgag
     1141 ctgtcccgct tcggcatccg cgtaatggcc atcgcccccg gcatcatgga gacgccgatg
     1201 ctgatgggca tgccgcaaga ggtgcaggac tcgctcggca agatggttcc cttcccctcg
     1261 cgtctgggca agcccgccga gttcgccgcg ctggtgcgcc acatcgtcga gaacgcctac
     1321 ctgaacggcg aggtgatccg cctcgacggc gcgatccgca tggccgccaa gtaggaaggc
     1381 gttgcaccgc agcagccgcc tgccggtgat gctgcggtgc aatagccggg gcgggtgaat
     1441 tatgttatta aaccggttcc aattcttccc gccccgcgca tctgcgctcg gggcggtttc
     1501 gcttggcgcc gatgactcct gtagaacagc ccgtttccgc ggccgccgcc gcagccgatg
     1561 ccgccgcgga tcgcgactgc tatcactgcg gtctgccgat tcctcccgag acgcggcact
     1621 acgtgcgcat cgacggccgc gagcggcgca tgtgctgcgt cggctgcgag gcggtggcgc
     1681 agtcgatcgt cgataacggc ctggtggatt actaccgcca tcgcgacgcg atgcccgaga
     1741 cgcggcgcga ggccatgccg gtcgaactgc aggagctcgg cctcttcgat caccccgact
     1801 tccagaagag cttcgtgcgc ccggtcggcg agcacgagcg cgaagccgcg ttgatcctcg
     1861 agggcatcac ctgcgccgcc tgcgtgtggc tcaacgagaa ccacgtcgcc cgcctgtccg
     1921 gcgtgtcggc gatcgagatc aactacgcca cccggcgtgc gcgcgtgcgc tgggacgagc
     1981 gccggatcaa gctttccgac atcctcgccg cgatccaggc gatcggctac cgcgcctatc
     2041 cctacgacgc cgagcgttcc gagcagctcg cccatcgtga gcggcgctcg atgctgtggc
     2101 gggtgttcgt tgccggcttc ggcatgatgc aggtgatgat gtacgccttc ccggtctacg
     2161 tggccggcga gggcgacatg acctgggaca tcgagcagct gctgcgctgg gccagcctgc
     2221 tgctgacgct cccggtggtg ctgtattcgg ccgcgccctt cttccagcgc gcctggcgag
     2281 acctgcgcct gcgccgcctg ggcatggacg tgccggtcgc gctcggcgtg ggcagcgcct
     2341 tcgccgccag cctgtgggcg acgctgaccg acgggccgga agtctatttc gattcggtga
     2401 cgatgttcgt gttcttcctg ctcggcgggc gctacctcga gatgatcgcg cgccagaagg
     2461 cggtacgcgg agtggaggaa ttgggcaaag ccttgccctc gttcgccgag cgcatcgccg
     2521 gctggccggg ggagtccgcc ggcgaacgcg tgccgacctc gcagctggtg gcgggggacg
     2581 tgctgcgtgt gcgcccgggc gaggtgatcc cggtcgatgg tctggtggtc gagggtcgcg
     2641 gcgaggccaa cgaggcgctg ctgaccggcg agagcatgcc ggtccccaag gcggtcggcg
     2701 atcgtgtcac cggcggcagt atcaacgtat cctcgccgct gctggtgcgc gtcgagcagg
     2761 tcggcgacgc cactcggctg gcggcgatcc gtcgcctcat ggaacgcgca gcgaccgaga
     2821 agccgcgcat cgcctcgctt tcggaccgca tcgccgccta cttcatcgtc accttgctgg
     2881 tgctggccgc gggaaccggg gtggcctggt acctgatcga tcccgcgcgt gcgctgtggg
     2941 tcttcgtgtc ggtgcttgtg gtcgcatgtc cgtgcgcgct ctcgctcgcc acgccgactg
     3001 cgctcaccgt ggccacggac acgctggcgc gcatgggtgt gctggtgacg cgcgggcatg
     3061 cgatcgaaac gctggcgcgg gccaatcgtt tcgtcttcga caagaccggc accctcacgc
     3121 tcggcaagat gcgggtggag gaggtgtgcg cgctcggcag cgtggacgcg gaccggctgg
     3181 tggtgctggc cgcggcgatc gagcagggtt ctgcgcaccc ggttgctgcc ggcctgcgcg
     3241 cggcggcggg tgaggcggcc ttgccggcgg tggccgagct ggctgccgag accgggcagg
     3301 ggatgcacgg cgtggtcgag ggcgagcgcc tctggatcgg ccgcccgggc tgggtcgcgg
     3361 gtgtcgccgg cctcgccgcg cctgctgcgc tcgcgggctt cgccgcgccc ggcggcacgg
     3421 tgatcgcact cgccgggcgg ggcggctggc tggggctgtt ccgccttggc gacagcctgc
     3481 gcgcggatgc gccgatgatc acccgtcgcc tggcggccga gggtgcggcg ctcggcctgc
     3541 tttccggcga cgcgcagccg gtggtggatg cggtcgccgc ccggctggga atccacgacg
     3601 cgcgcggtgg cctggatccg cagggcaagc aacaggcgat cgtcgacatg cagcgcgacg
     3661 acagggcggt cgtggcgatg atcggcgacg gcgtgaacga cgccccggta ctcgcccagg
     3721 cccacgtttc ggtggcgatg ggcggcggca ccgacctcgc gcgcaaccag gccgacatcg
     3781 tgctgctcaa cgagcgcttc gtcagccttg ccgacgggat cgtgcttgcg cgcaggaccc
     3841 tgcgcatcat ccggcagaac ctgtggtggt cgttcgccta caatttcacc tcggtgccgc
     3901 tcgccatggc ggggctggtg accccgtgga tggccggcat cggcatggcg gcgagttcct
     3961 tgctcgtcgt gctcaacgcg atgcgcctgc agcgcgccac gcgcaccgag cgcgcaggcg
     4021 cggagaactg agatggaaag cctctacttg ctgattccgc tgtcggtcgt gctggtcttc
     4081 gtcatcgggg tgttgttctg gtggtcgctg cgcagcggcc agtacgacga cctcgagggt
     4141 ccggcctacc ggctgctgat ggacgaccgc gaacaactcc cgggggacgg gcaggagcgg
     4201 gatgcagacc cgtccggcga ggtggctgcg aatggggttt ccgatcgccg taccggttcc
     4261 gcgggtgagc aatcgaggtt tcgttgacct gcgtcaactg ctgtctcatg taacggtgga
     4321 aaatatcggc gttgcgaccg atcgcctttc atgacgatgg cgagcggtcc ggggtttatg
     4381 tctctctgtt ggggttgggg gtgcgtgcag acgcaccctt ttttttgcct ctcgaaaggc
     4441 atgccgaaac cgctggatgc cttgcaggca ttggcttcca gcccgctcgc acgattgcgc
     4501 gccgcgctcg ttgctgggtt ttgggtttgt ttttggacta actccaattt ccccttcctt
     4561 ggccccgtct gctgcaatac aatattgact tgtgtcaaat tgcgtatgtg gtcgtggcgt
     4621 attctcgccc gagtggttcg ccgcggaagt ctgctgcggc gcgacgcaag caggggtttt
     4681 caaaaacaaa gaggtgatcc agatgcaatc gcaagcgact tataactata aagtcgtgcg
     4741 ccagttcgcc atcatgacgg tggtgtgggg catcgtgggc atgctcgtcg gcgtgatcgc
     4801 cgcggcacag ctcgtgtggc cccagttgaa cgtgcacgaa ttcctgcatt tcggcaggct
     4861 ccgtccgctg cacaccaacg cggtgatctt cgcgttcggt ggctgcgcgt tgttcgcgac
     4921 gtcgtactac gtcgtccagc gcacttgtca caccagactg ttcgcgccgg gcctggccgc
     4981 gttcaccttc tggggctggc agctggtcat cctgcttgcc gcgatcaccc tgccgctggg
     5041 ttacaccacc tccaaggaat acgccgagct cgaatggccg atcgacatcc tgatcgcgat
     5101 cgtgtgggtg tcctatgcca tcgtgttctt cggcaccatt gccaggcgca aggtctcgca
     5161 catctatgtg gcgaactggt tcttcggcgg cttcatcctc accgtcgcgc tgctccacat
     5221 cgtgaacagc gccgcgatcc ccgtctcgct gaccagcatg aagtcctatt cggcctatgc
     5281 cggcgtgcag gacgcgatga tccagtggtg gtacgggcac aacgcggtgg gcttcttcct
     5341 gactgcgggc ttcctcggca tgatgtacta cttcgtgccc aagcaggccg accgtccggt
     5401 gtattcctat cgcctgtcgg tggtgcactt ctgggcgctg atcttcacct acatgtgggc
     5461 gggtc
//
LOCUS       DAOQBS010000161         5405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_15593_length_5405_cov_15.933271, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000161 DAOQBS010000000
VERSION     DAOQBS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15593_length_5405_cov_15.933271"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1251
                     /locus_tag="PKN25_13775"
     CDS             106..1251
                     /locus_tag="PKN25_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNV92016.1"
                     /translation="MSADEGRAALVLTGGGARAAYQVGVLAAVRELRGKRPGNPFPIL
                     CGTSAGAINAVALAVHAGDFGRAVRHIQRVWRSLHVDQVYRVDPLALFGSGLRWGAAL
                     FGGWLVRQTPRSLLDNTPLRGLLERVLDFPAIEAAIGRGDLRALSVTASGYSSGESLA
                     FFQGAPELQPWRRAQRLGVRAELDVCHVLASAALPFVFPAVKIHREYFGDGSMRQLAP
                     VSPAIHLGADRILVIGAGRTAEEGRVRAAGGYPSPAQIAGHALSSIFLDTLEVDLERL
                     ARINDTVGRLDEARRAEAGIALRPLECLVIQPSQRLDIIAARHAHSLPAQLRIVLRGL
                     GTFRREGSTLLSYLLFEPSFTRALIELGYHDAMTRAGELRRFLRIDS"
     gene            1411..2577
                     /gene="pbpG"
                     /locus_tag="PKN25_13780"
     CDS             1411..2577
                     /gene="pbpG"
                     /locus_tag="PKN25_13780"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HNV92017.1"
                     /translation="MKVRPLIAAIAAMFALQSGMAIAAPKASGKAATSKVSKPAAKKA
                     AATAPVRKAAVKPAPKKGVVAKATASDTKAARSAKAATRASANARGKAPTMRVSLRKP
                     AVAAAALAAPAAVLADEPDTPVALDGSGMPVLASNAFYVINQRTGEVLLERNATEVLP
                     IASITKLMTAMVVLDAGLSLSEPIEINDADIDQLKGTGSRLALGTTLSREEMLQLALM
                     SSENRAASSLARHYPGGEAAFVDAMNVKARLLGLWNTRFHDSTGLNPANVSSPRDLAK
                     MVAASASYPLIREFSTAHERYVPVHGRMLRFGNTNSLVRSPEWTISVSKTGYISEAGR
                     CLVMQTWLHQQPVVMVLMDSSGRYTRTADAKRVRKWLEQTAPQRLAAAPQGAGG"
     gene            2808..4682
                     /locus_tag="PKN25_13785"
     CDS             2808..4682
                     /locus_tag="PKN25_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV92018.1"
                     /translation="MGAALPETAPSRLFLSGNEAVALAVRDAGCRVAAAYPGTPATEI
                     LEELSRFPDLYTEWSVNEKVSLEVALGASMVGARAFCAMKHVGLNVAADALMTMTVTG
                     TEGGLVIAVADDVGMSSSQNEQDSRFWARFAHLPLFEPADAQESYDMAREAFAVSERF
                     RCPAIVRLTTRICHVKGRVVAGEREAHEPAGFVKDPQRWVMVPGHAKPRVALMYEREE
                     ALRAASAQSPFNLLVEGSDRRIGFVTSGPAYMHVRETFPDAPVFKLGQSYPLPLERLR
                     EFAAGVDQLLVVEETEPLVESELRAGGIACTGKDVLPRVGELSPDRLRPAVARLRGEE
                     IPVTAQPRLVPQQVFPRPPTMCVACPHLGIYYTLAQLRNLTISGDIGCYTLGAGHPWN
                     ALDTCISMGASMGVALGMDKGRGQADAKKAVIAVIGDSTFMHMGMQGLLDITWNRGNV
                     TVLLLDNRAVGMTGGQDNPGTGRDIHGESAQRVDFAKLCEALGVKKERIHTLDPYELP
                     TLFKTLREEIKIPEPSVIITDRPCVLIDHYKPTQPYKVIADKCTGCANCIDVGCPAIH
                     VTRRETQVKPSGKEVELAFVRIETSACTGCGLCVQPCAPEAIVHALPEHPVKFLHAKV
                     "
     gene            4693..5307
                     /locus_tag="PKN25_13790"
     CDS             4693..5307
                     /locus_tag="PKN25_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNV92019.1"
                     /translation="MTITNIAVCGIGGQGVMTATEILAEAALSLGFDVKKTEVAGMSQ
                     RGGVVTSHLRFGPQVLSPQIMPGEADLLVGFEAAEALRWAHMLKPGGIALVNQGRFVP
                     PVVTIGLYDYPEDPIGEMRKLGLAVFDFDASALALQLGNIRLGNTVMLGAMSDRLPFP
                     ADVLRNAVLARFGARKPELLELNRQAFELGRAAVAQPAPAPATA"
BASE COUNT          769 a         2036 c         1811 g          789 t
ORIGIN      
        1 accgaccacg cccctcgcta ccgcaccgcg cccgcgcatt ccttccttgt ccggtcggcc
       61 tgcgctgcgc gaagctgacg ccatcccatg gccggaggcg ggcggatgag cgcggacgaa
      121 gggcgggcgg cgttggtgct gaccgggggt ggcgcgcgcg ccgcctacca ggtcggcgtg
      181 ctggctgcgg tccgcgaact gcgcggcaag cgccccggca accccttccc gatcctgtgc
      241 ggcacctcgg cgggggcgat caacgcggtc gcgctcgccg tgcacgcggg cgacttcggc
      301 cgtgcggtgc ggcacatcca gcgcgtctgg cgcagcctgc acgtggatca ggtgtaccgc
      361 gtcgatccgc tcgcgctctt cggcagcggc ctgcgctggg gcgcggcgct gttcggcggc
      421 tggctggtcc ggcagacgcc acgctcgctg ctcgacaaca cgcccttgcg cggcctgctc
      481 gagcgcgtgc tcgacttccc cgcgatcgag gccgcgatcg gccgcggcga cctgcgcgcg
      541 ctcagcgtca ccgcctcggg ctattcctcc ggcgagagcc tcgccttctt ccagggcgcg
      601 cccgagctgc agccctggcg gcgcgcgcag cgcctgggcg tgcgcgccga gctcgacgtc
      661 tgccacgtgc tggcgagcgc ggcgctgccc ttcgtcttcc ccgcggtgaa gatccatcgc
      721 gagtatttcg gcgacggctc gatgcgccag ctcgcgccgg tgagcccggc gatccacctc
      781 ggcgccgacc gcatcctggt gatcggcgcc ggacgcaccg ccgaggaggg gagagtgcgt
      841 gcagcgggcg gctacccgtc gccggcgcag atcgccggcc acgccttgtc gagcatcttc
      901 ctcgacaccc tcgaggtcga tctcgagcgc ctcgcgcgca tcaacgacac ggtgggccgc
      961 ctcgacgagg cccgccgcgc cgaggcgggg atcgcgctgc gtccgctcga gtgcctggtg
     1021 atccagccct cgcagcgcct cgacatcatc gccgcgcgcc atgcgcacag cctgccggca
     1081 cagctgcgca tcgtgctgcg cgggctgggc acctttcgcc gcgagggctc gaccctgctc
     1141 tcctacctgc ttttcgaacc gagcttcacg cgcgcgctga tcgagctcgg ctatcatgac
     1201 gcgatgacgc gcgccggcga actgcgccgc ttcctgcgca tcgactcctg acccggggcg
     1261 ggggtggcga gcggactcgc cggcgacgcc gctgcgggag tagaattccg ccagagttgg
     1321 aaacaacttg aaatccattt cgtatctgcc tattttttaa agcagaacgc ctcgaatccg
     1381 ccttccaccg ctctccgtcc cgtcgccatc atgaaagttc gccccctgat cgccgcgatt
     1441 gccgcgatgt tcgcgctgca gtccggcatg gccatcgcag ccccgaaggc atccggcaag
     1501 gctgcgacga gcaaggtttc caagccggcc gcgaagaagg ccgcggccac cgccccggtg
     1561 cgcaaggccg ccgtcaagcc cgcgccgaag aagggcgtcg tggccaaggc cacggcgtcc
     1621 gacaccaagg cggcccgttc cgccaaggct gcgacccgtg cgtccgccaa cgcccgcggc
     1681 aaggcgccga cgatgcgggt gagcctgcgc aagccggcgg tcgccgccgc cgcgctcgcc
     1741 gcccccgccg cggtgctcgc cgacgaaccg gataccccgg tcgcgctcga cggcagcggc
     1801 atgcccgtgc tcgcttcgaa cgccttctac gtgatcaacc agcgcaccgg cgaggtgctg
     1861 ctcgagcgca acgccaccga agtgttgccg atcgcctcga tcaccaagct gatgaccgcg
     1921 atggtggtgc tcgatgcggg gctgagcctg tccgagccga tcgagatcaa cgacgccgac
     1981 atcgaccagc tcaagggcac cggctcgcgg ctcgcgctcg gcaccacgct gtcgcgcgaa
     2041 gagatgctgc agctcgcgct gatgtcgtcc gaaaaccgcg cggcctcgtc gctggcccgc
     2101 cactatccgg gcggcgaggc cgccttcgtc gacgcgatga acgtcaaggc gcgcctgctc
     2161 gggctgtgga acacccgctt ccatgacagc accgggctca acccggccaa cgtctccagc
     2221 ccgcgcgacc tcgccaagat ggtcgcggcc tcggcgtcct acccgctgat ccgcgagttc
     2281 tccaccgcgc acgagcgcta cgtgccggtg cacggccgca tgctgcgctt cggcaacacc
     2341 aactcgctgg tgcgcagccc ggaatggacg atcagcgtgt ccaagaccgg ctacatctcc
     2401 gaggcggggc gctgcctggt catgcagacc tggctgcacc agcagccggt ggtcatggtg
     2461 ctgatggatt cctccggccg ctacacccgc accgccgacg ccaagcgcgt gcgcaagtgg
     2521 ctcgaacaga ccgctcccca gcgcctggcg gccgcaccgc agggcgcggg cggctgagct
     2581 tcccgattac gcaggtcgaa cgacgcgggg ccgcccggtg gggcaggccc cgcgtcgcgt
     2641 ttggtctgat cgagcgcact gccgctgctg gcgcggcatc ggcgcccgtc gcgggtgctc
     2701 cgtggcgacc acgactgcag ggcggttgat cttcgtcaat ggagccgggc agggcgctca
     2761 ctaagctcgc gggttccgac cccgaaccca ccagagagga tctccccatg ggtgctgccc
     2821 tgcccgaaac cgccccgagc cgcctgttcc tgtccggcaa cgaggccgtg gcgctcgccg
     2881 tgcgcgacgc cggctgccgc gtcgccgccg cctatccggg cacgccggcg accgagatcc
     2941 tcgaggagct gtcgcgcttt cccgacctgt acaccgagtg gtcggtgaac gagaaggtct
     3001 cgctcgaggt cgcgctcggc gcctcgatgg tgggcgcgcg cgccttctgc gcgatgaagc
     3061 acgtcggcct caacgtcgcc gccgacgcgc tgatgaccat gaccgtcacc ggcaccgaag
     3121 gcggcctcgt gatcgcggtc gccgacgacg tcggcatgtc ctcgtcgcag aacgagcagg
     3181 actcgcgctt ctgggcgcgc ttcgcccacc tgccgctgtt cgagcccgcc gacgcgcagg
     3241 agtcctacga catggcgcgc gaggccttcg cggtgtccga gcgcttccgc tgtccggcga
     3301 tcgtgcgcct gaccacccgc atctgccacg tcaagggccg cgtcgtcgcg ggcgagcgcg
     3361 aggcccacga gcccgccggc ttcgtcaagg atccgcagcg ctgggtcatg gtgcccggcc
     3421 acgccaagcc gcgcgtggcg ctgatgtacg agcgcgagga ggccttgcgc gccgcctccg
     3481 cgcagagccc gttcaacctg ctcgtcgagg gcagcgaccg ccgcatcggc ttcgtgacct
     3541 cgggcccggc ctacatgcac gtgcgcgaga ccttccccga cgcgccggtg ttcaagctcg
     3601 gccagtccta cccgctgccg ctcgagcgcc tgcgcgagtt cgccgccggc gtcgaccagc
     3661 tcctggtggt cgaagagacc gagcccctgg tcgagagcga gctgcgtgcc ggcggcatcg
     3721 cctgcacggg caaggacgtg ctgccgcgcg tcggcgagct ctcgcccgac cgcctgcgcc
     3781 cggccgtggc ccgcctgcgc ggcgaggaaa tcccagtcac cgcgcagccc cgcctggtgc
     3841 cgcagcaggt cttcccgcgc ccgcccacca tgtgcgtcgc ctgtccgcac ctgggcatct
     3901 attacaccct cgcgcagctg cgcaacctca ccatctcggg cgacatcggc tgctacaccc
     3961 tgggcgccgg ccacccctgg aacgcgctcg acacctgcat ctcgatgggc gcctcgatgg
     4021 gcgtcgcgct cggcatggac aagggccgcg gccaggccga cgccaagaag gcggtgatcg
     4081 cggtgatcgg cgactccacc ttcatgcaca tgggcatgca gggcctgctc gacatcacct
     4141 ggaaccgcgg caacgtcacc gtgctgctgc tcgacaaccg cgcggtgggc atgaccggcg
     4201 gccaggacaa ccccggcacc ggccgcgaca tccacggcga gagcgcgcag cgggtcgact
     4261 tcgccaagct gtgcgaggcg ctgggggtga agaaggagcg catccacacg ctcgacccct
     4321 acgagctgcc gacgctgttc aagaccctgc gcgaggagat caagatcccc gagccctcgg
     4381 tgatcatcac cgaccggccc tgcgtgctga tcgaccacta caagcccacg cagccctaca
     4441 aggtgatcgc ggacaagtgc accggctgcg ccaactgcat cgacgtcggc tgcccggcga
     4501 tccacgtcac ccggcgcgag acccaggtca agccctcggg caaggaagtc gagctcgcct
     4561 tcgtgcgcat cgagacctcc gcctgcaccg gctgcggcct gtgcgtgcag ccgtgtgcgc
     4621 ccgaagccat cgtccatgcc ctgccggagc atccggtgaa gttcctgcac gccaaggtct
     4681 gaggaggccg ccatgaccat caccaacatc gcggtttgcg gcatcggcgg ccagggtgtg
     4741 atgaccgcca ccgagatcct cgccgaggcg gcgctgtcgc tcggcttcga cgtcaagaag
     4801 accgaggtcg ccggcatgag ccagcgtggc ggggtggtga cctcgcacct gcgcttcggt
     4861 ccgcaggtgc tgtcgccgca gatcatgccg ggcgaggccg acctgctcgt cggcttcgag
     4921 gccgccgagg cgctgcgctg ggcgcacatg ctcaagccgg gcgggatcgc gctggtcaac
     4981 cagggtcgct tcgtgccgcc ggtggtgacg atcggcctct acgactaccc cgaggacccg
     5041 atcggcgaga tgcgcaagct cgggctggcg gtgttcgatt tcgacgccag cgcgctcgcg
     5101 ctgcagctcg gcaacatccg cctcggcaac accgtgatgc tgggcgccat gtccgaccgc
     5161 ctgcccttcc cggccgacgt gctgcgcaac gcggtgctgg cgcgcttcgg cgcccgcaag
     5221 cccgagctgc tggagctcaa ccgccaggcc ttcgagctcg gccgcgccgc agtcgcccag
     5281 cccgcccccg ccccggccac cgcctgaccc atcgcggcgc gcaacgcatc cgggcgtcgg
     5341 cgttcgcgcc acgcgtccgg gtgtcggtgt cttgccgtgg gagcgacgca agtcgcgatt
     5401 gcagc
//
LOCUS       DAOQBS010000162         5360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_15792_length_5360_cov_14.107257, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000162 DAOQBS010000000
VERSION     DAOQBS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15792_length_5360_cov_14.107257"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PKN25_13795"
     CDS             <1..818
                     /locus_tag="PKN25_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV92020.1"
                     /translation="RFSANKSKAKQATSRLKLIDKLKPEDVKPSSRQYPWIRFDYDDK
                     DKLHRLACEVDNISFAYPGMDKPLINRFSIAIEAGEKVAIIGENGVGKTTLLKLLVGE
                     LAPQKGAIKWAEKAKPGYYAQDHSADFAEDVSLTDWIAPYSRITAAATGEDNETLLRG
                     TLGRLLFSGDEVKKSVKVISGGEQGRMLFGKLMLSRPNVLLMDEPTNHLDMESIESLN
                     TGLDKFNGTLVFVSHDREFVSSLATRIIEIKLDHQIVDYRGTYEEYLQSQGLD"
     gene            1028..>1279
                     /locus_tag="PKN25_13800"
     CDS             1028..>1279
                     /locus_tag="PKN25_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNV92021.1"
                     /translation="MIASEIPELALRVETDRFGPFLFQVGVDTGRLQLPMQRVEDAHR
                     RFVASPLAQVANQLEREVIASSIYGTNTIEGGTLTEDETR"
     assembly_gap    1280..1379
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1380..2129
                     /locus_tag="PKN25_13805"
     CDS             <1380..2129
                     /locus_tag="PKN25_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNV92022.1"
                     /translation="RPGLLRDTPKGRTTQVGSPQHGGRYKPPQYGGDIWRLLEALITW
                     HGQLVDAGIHPLVRAPLVHYYYELIHPFWDGNGRVGRVVEATLLQAAGYEYAPFALAR
                     YYLEHIDAYFTLFNTCRKGADKHRPHPNTDFVLFHQEGMLATIDALHDRVNRLVGVLL
                     FQSRCRELLDNKTLNPRQYTIVSQLLAQGQAQGLDAVRHAPWYASLYLKLNDKTRSRD
                     LKKLRELELVFLDTDNRLWPGCIRPKNVKAP"
     gene            2623..3012
                     /locus_tag="PKN25_13810"
     CDS             2623..3012
                     /locus_tag="PKN25_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HNV92023.1"
                     /translation="MIGLFLVTHGTLGEALIQCVCHVLDKRPPLLGQLGLSAGEDPAD
                     ALPLARRMIATVDDGEGVLLLADLYGATPCNVATRLIEPGRIELVAGVSLPMLLRVLT
                     HRDGNLDTLIRRAVSGAGDGVTRVQPG"
     gene            3066..3335
                     /locus_tag="PKN25_13815"
     CDS             3066..3335
                     /locus_tag="PKN25_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNV92024.1"
                     /translation="MPRGDAPIINKLGLHARASAKLTQLASSFPCEVWLERNGRRVNA
                     KSIMGVMMLAAARGSTITIDTQGEGADAALQAIQELVADRFGEGE"
     gene            3337..5067
                     /gene="ptsP"
                     /locus_tag="PKN25_13820"
     CDS             3337..5067
                     /gene="ptsP"
                     /locus_tag="PKN25_13820"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNV92025.1"
                     /translation="MSFTLHGLAVSQGIAIGHVHLVSHALLEVNHYHVAPRYLDDELA
                     RLDEALATVQGELIGLKAITTSGQAHSEVGAFVDLQLMMLADPMLVDAARQLVTERRC
                     NAEWALVQQMEHLVEQFRQIEDPYLRERQADVVQVVERLVKVLLGHPGHLPPRRRDGL
                     GSIVVAHDLSPADTIGFRDHNIAGFVTDVGGPTSHTAIVARSLKIPAVVGLHHVRDLL
                     EDDELIIVDGTRGVIIVAPDEQIVEEYRLRRSELEIERSKLNRLRDTRAATLDGEEVN
                     LLANIEGPKDLPAVKSANADGIGLYRTEFLFIGRDTLPDEEEQYEAYRAVVKALPGKP
                     VTIRTFDVGADKALNGAQAREEPNPALGLRAVRYSLAEPKMFKTQLRALLRASAHGRL
                     QIMVPMLAHAQEVDQCLTLLEKAKAELRAEKIKFDEGIRIGGMIEVPAAALSLGMFIR
                     RLAFLSIGTNDLIQYTLAIDRSDEAVAHLYDPLHPAVLKLIAGTISSGARFGLPVSVC
                     GEMAGDPLYTELLLGMGLRNFSMHPGSILEIKQQVLRADLGELAPRVQRILKMDEPAR
                     IREAVERLTA"
     gene            5150..5224
                     /locus_tag="PKN25_13825"
     tRNA            5150..5224
                     /locus_tag="PKN25_13825"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5183..5185,aa:Gly,seq:ccc)
BASE COUNT          946 a         1898 c         1610 g          806 t
ORIGIN      
        1 gccgcttctc ggccaacaag tccaaggcca agcaggccac cagccgcctc aagctcatcg
       61 acaagctcaa gcccgaggac gtcaagccgt cttcccgcca gtacccgtgg atccgcttcg
      121 actacgacga caaggacaag ctgcaccgcc tcgcctgcga ggtggacaac atctcgttcg
      181 cctatccggg catggacaag ccgctgatca accgtttctc gatcgccatc gaggccggcg
      241 agaaggtcgc gatcatcggc gagaacggcg tcggcaagac caccctgctc aagctgctgg
      301 tgggcgagct tgcgccgcag aagggcgcca tcaagtgggc ggagaaggcc aagccgggct
      361 actacgcgca ggaccactcg gccgacttcg ccgaggacgt cagcctcacc gactggatcg
      421 cgccctactc ccgcatcacc gccgccgcca ccggcgagga caacgagacc ctgctgcgcg
      481 gcacgctcgg ccgcctgctg ttctcgggcg acgaggtgaa gaagtcggtg aaggtgatct
      541 ccggcggcga gcagggccgc atgctgttcg gcaagctgat gctgtcgcgc cccaacgtgc
      601 tgctgatgga cgagcccacc aaccacctcg acatggaatc gatcgagtcg ctcaacaccg
      661 gcctggacaa gttcaacggc acgctggtgt tcgtctccca cgaccgcgag ttcgtgagct
      721 cgctggctac gcgcatcatc gagatcaagc tcgatcacca gatcgtggat taccgcggga
      781 cgtacgaaga gtatctccag tcccagggcc tggactgatc cgggcggctc cggccgcggt
      841 tgcacgacaa ggggcgaacc cgctgccgcg cgatgcggcg gccggttcgc cccttcgctt
      901 ttgccgagcc gggtccatca ggcctcgagc gctggacatc cggacggaca cggatcgccg
      961 ataacgcgcg ccatctcatt gaaaatagaa taaaattctg tttttttgag cggacattcc
     1021 ggcagacatg atcgcctccg agattcccga gctcgcgctg cgcgtcgaaa ccgaccgctt
     1081 cggccccttc ctctttcagg tgggcgtcga cactggccgc ctgcagcttc cgatgcagcg
     1141 cgtcgaggat gcccatcggc gcttcgtcgc ctcgccgctc gcgcaggtgg cgaatcaact
     1201 cgagcgcgag gtgatcgcca gcagcatcta cgggaccaac acgatcgaag gcggcacgct
     1261 caccgaggat gaaacacgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1381 gccccggcct tctccgcgac acccccaagg gacgcaccac ccaggtcggc agtccgcagc
     1441 acggcggacg ctacaagccg ccacaatacg ggggcgacat ctggcgcctg ctcgaagcac
     1501 tgatcacctg gcacggccag ttggtggatg caggcatcca cccgctcgtc cgcgcgccgc
     1561 tggtgcatta ctactacgaa ctcatccacc ccttctggga tggcaacggc cgtgtcgggc
     1621 gcgtcgtcga agcgaccttg ctgcaggccg ccggctacga atacgcccct ttcgcgctgg
     1681 cgcgctacta cctcgagcac atcgacgcct acttcacgct cttcaacacc tgccgtaaag
     1741 gggcggacaa gcaccggccc cacccgaaca cggacttcgt gctctttcac caggaaggca
     1801 tgctggcgac gatcgacgcc ttgcacgacc gggtcaatcg cctcgtcggc gtcctgctct
     1861 tccagagccg ttgccgcgag ctgctcgaca acaagaccct caacccgcgc cagtacacca
     1921 tcgtctcgca actgttggcg caggggcagg cgcaggggct ggacgcggta cgtcacgccc
     1981 cgtggtacgc cagcctctac ctcaagctca acgacaagac ccgcagccgc gacctcaaga
     2041 agctgcgcga gctcgaactc gtcttcctcg acacggacaa ccgcctgtgg ccgggctgca
     2101 tccgtccgaa gaacgtcaag gcgccctgat cccgcatctg caggttcgcg gcggtcctgg
     2161 cgagtcgagc gcaattccgc acgatggagg agcgcccggg tccagcgtgg acgcacggca
     2221 ggcggttcgg caccagggtg agcaccacga aggcgaccac cagcaggccg gacaccgcga
     2281 acacgcggtt gtggatgtcg aggccgaagg gcccgacgtt gaggacgatg ttgtcctggc
     2341 cgaccttgta gtcggtgtcg atcggattga ccgcccccga gggggccatc ggatcgctgc
     2401 catccgccat tgcagctcct tgtcattatg ggaattcgtg gaattcacgc gcacggccga
     2461 gccttcgccg cggcgacgag gggcggagtc ttgcagcttt ctacatattt ttgtacaaac
     2521 agatcatgac agatgggatt tcgcctcgcc cgcgcactcg atccgcccgc agcggcacgg
     2581 cgggcggcga gtcggctaga atcgaaggct gcggagaacc ccatgatcgg cctctttctc
     2641 gtcacccacg gcaccctcgg cgaagcgctg atccagtgcg tgtgccacgt cctcgacaag
     2701 cgcccgccgc tgctcggcca gctcggcctg tcggcgggcg aggatcccgc cgacgcgctg
     2761 cccctcgcgc gccggatgat cgccacggtc gatgacggcg aaggcgtact gctgctcgcc
     2821 gacctctacg gcgccacccc gtgcaacgtc gccacgcgcc tgatcgagcc cgggcgcatc
     2881 gagctcgtcg ccggagtcag cctgccgatg ctgctgcgcg tgctgaccca tcgcgacggc
     2941 aacctcgaca ccctgatccg gcgcgccgtc agcggcgcgg gcgacggcgt gacgcgcgtg
     3001 cagcccggct gatcacaccc gccaacaaga acatacgaac gaacgcacca cggagaaccg
     3061 cgaagatgcc gcgaggagac gcccccatca tcaacaagct cggtctgcac gcgcgcgcct
     3121 cggccaagct cacccagctt gccagcagct ttccgtgcga ggtctggctc gagcgcaacg
     3181 gccgccgggt gaacgcgaag agcatcatgg gggtgatgat gctggcggcc gcgcgcggca
     3241 gcacgatcac catcgacacc cagggcgagg gcgccgacgc ggcactgcag gccatccagg
     3301 agctggtcgc cgaccgcttc ggcgaagggg aatgagatgt cattcacgct gcacggcctg
     3361 gcggtctcgc agggcatcgc gatcggccat gtccacctcg tctctcacgc gctgctcgag
     3421 gtcaatcact accacgtcgc gccgcgctac ctcgacgatg aactcgcccg cctcgacgag
     3481 gccctcgcca ccgtccaggg cgagctgatc ggtctcaagg cgatcaccac ctccggccag
     3541 gcgcacagcg aggtcggcgc cttcgtcgat ctgcagctga tgatgctcgc cgacccgatg
     3601 ctggtcgacg ccgcccgcca gctcgtcacc gagcggcgct gcaacgccga gtgggcgctg
     3661 gtgcagcaga tggagcacct ggtcgagcag ttccgccaga tcgaggaccc ctacctgcgc
     3721 gaacgccagg ccgatgtcgt ccaggtcgtc gagcgcctgg tcaaggtgct gctcggccac
     3781 cccggccacc tgccgccgcg tcggcgcgac gggctgggca gcatcgtcgt cgcccacgat
     3841 ctgtcgcccg ccgacaccat cggcttccgc gaccacaaca tcgccggctt cgtcaccgac
     3901 gtcggcggcc ccaccagcca caccgcgatc gtcgcgcgca gcctcaagat ccccgcggtg
     3961 gtcggcctgc accacgtgcg cgacctgctc gaggacgacg agctcatcat cgtcgacggc
     4021 acccgcggcg tgatcatcgt cgcccccgac gagcagatcg tcgaggaata ccgcctgcgc
     4081 cgcagcgagc tcgagatcga gcgttccaag ctcaaccgcc tgcgcgacac ccgcgccgcc
     4141 acgctcgacg gcgaggaggt caacctgctc gccaacatcg agggccccaa ggacctgccc
     4201 gcggtcaaga gcgccaacgc cgacggcatc ggcctgtacc gcaccgagtt cctcttcatc
     4261 ggccgcgaca ccctgcccga cgaggaagag cagtacgagg cctaccgcgc cgtggtcaag
     4321 gccctgcccg gcaagccggt caccatccgc actttcgacg tcggcgccga caaggcgctc
     4381 aacggtgccc aggcgcgcga ggaacccaac ccggcgctcg gcctgcgcgc ggtgcgctac
     4441 tcgctggccg agcccaagat gttcaagacg cagctgcgcg cgctgctgcg cgcctcggcc
     4501 cacggccgcc tgcagatcat ggtgccgatg ctcgcccacg cccaggaggt cgaccagtgc
     4561 ctgaccctgc tcgagaaggc caaggccgag ctgcgcgccg aaaagatcaa gttcgacgag
     4621 ggcatccgca tcggcggcat gatcgaggtg cccgccgcgg cgctgtcgct cggcatgttc
     4681 atccggcggc tggccttcct gtcgatcggc accaacgacc tcatccagta caccctggcg
     4741 atcgaccgct cggacgaggc cgtcgcgcac ctctacgacc cgctccaccc cgccgtgctc
     4801 aagctcatcg ccggcaccat ctccagcggc gcgcgcttcg gcctgccggt gtcggtgtgc
     4861 ggcgagatgg cgggcgaccc gctgtacacc gagctgctgc tcggcatggg cctgcgcaac
     4921 ttctcgatgc acccgggcag catcctcgag atcaagcagc aggtgctgcg cgccgacctc
     4981 ggcgagctcg cccccagggt gcagcgcatc ctcaagatgg acgagccggc acgcatccgc
     5041 gaggcggtgg aacggctcac cgcctgaaaa caccgccgcg gttgaagcgc accggccgtg
     5101 gcagtacaat ccggccttct tcgcggttga cgccaggtcg gcccgaaatg cgggtgtcgt
     5161 ataatggcta ttacctgagc ttcccaagct catgacgagg gttcgattcc cttcacccgc
     5221 tccaccgcat cattctgcgg acttccgcag aagtccaaga gagtctgcaa gtggttgcca
     5281 cacaaggact tttcctctct ttgatgttct gccgtagtcc actgcgatcc gcctacagcc
     5341 gggactttta agtccacaaa
//
LOCUS       DAOQBS010000163         5352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_15832_length_5352_cov_15.849160, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000163 DAOQBS010000000
VERSION     DAOQBS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15832_length_5352_cov_15.849160"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PKN25_13830"
     CDS             complement(<1..663)
                     /locus_tag="PKN25_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HNV92026.1"
                     /translation="MSLHSSPGLVFDTPPIELETGPDPRYSVIWMHGLGADGSDFEPV
                     VPALALPRSPAVRFLFPHAPYRAVTCNAGYVMRAWYDIVSLAPHSRQIDEAGLLESRT
                     LVRQLIQREADRGVPGERVILAGFSQGGAVAYLTGLTHPTPLAGIIALSTYIPSPGLL
                     VDGFVEANRQVPVFAAHGTHDDVVSPALGEHAVEVLRQLGIEPEWHRYPLPHSVSLEE
                     IAD"
     gene            762..1007
                     /locus_tag="PKN25_13835"
     CDS             762..1007
                     /locus_tag="PKN25_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HNV92027.1"
                     /translation="MNPEHLVLLLEREMPFGKYKGRVIADLPGYYLAWFARKGFPPGQ
                     LGQLLALMHELDHNDLAGLLEPLRRARRQAAAGPGGV"
     gene            1125..2249
                     /locus_tag="PKN25_13840"
     CDS             1125..2249
                     /locus_tag="PKN25_13840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HNV92028.1"
                     /translation="MPRFLHTADWQIGRQYARFAPEDALALAEARFSAVERLAGIARQ
                     EAVDAVLVAGDVFDAQTVSDRTIRRLFNAMGAYAGPWVLIPGNHDAALAESVWSRARR
                     LGAVAPNVHLALGAQVMAFPERGFVVLPAPLTQRQTPADETAWFDHAETAPGLVRVGL
                     AHGSVQGVLAEDIDSPNPIAADRATRAALDYLALGDWHGTRRIDARTWYSGTPEQDRF
                     KANDPGNVLLVDIPAVGAEPEVEVRAVGRHPWIALEEKLAVASDLEGTLARLEALPEL
                     AVVDLSLSGEIHLLGRQRLEAALSVAEARFRSLRADLSGLRLQPTEDDLAALHADGYV
                     GRVIAELREEQDGENAEVASEALAILAALLLEAQAQGAAA"
     gene            2246..4882
                     /locus_tag="PKN25_13845"
     CDS             2246..4882
                     /locus_tag="PKN25_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV92029.1"
                     /translation="MRLLRLKVEQLRRFRQPVEIRDLDPGINLFTGPNESGKSTLVRA
                     IRAAFFERYKSSSVEDLQPWGDSAAAPTVELEFESQGKRWKLVKSFLKRKRCDVSVDG
                     VGFSGEEAEDKLADLLGFEFPGRGASRPEHWGIPGLLWIEQGAGQEIHKPVEHAGSHL
                     KSVLGASLGEVTSTAGDELIARITRERARLLTATGRPTGDFAAALEAHERLAREHADL
                     QGRIRQYRQQVDRLGQLREEQARDESERIWEVYRRQARAADLRLAEVVSWQREQERDQ
                     QALQACQAGMRTSLDLLGRFEAQRKDLHRRAADRQAAEQALAAQDARRPAVERVLGDA
                     RKAFEAARATVRLAREHQQRKDLVRELAQVDGELAGLDQALARARELHERWLRQRALR
                     QSLQVDAKALKRLRKVEEELARLAIRQEAVATRLQFDLREAGALILGGEPLSGRGERL
                     LLEPTEILVAQLGSLRILPGGDDIADLVRTRQGLADERASLLSALGADSLAAADARHE
                     QCRELDESIKRDASVLESLAPEGLEALAGRKQLTQARREDLGKRIGALPEPQEDSLSV
                     SRAEAQLDAAEVSLKAAEADEAGFRSALLLATQALESARAEWGRLDGELQGADRKARE
                     KQAEDQLLELRAGENRLDQALRERQKQIDQANPGVLRQDVERFTRSADAAEKAAEQRR
                     LELERLRASLEALGAEGLEERSAELDVELARAARRSDELTRRARALDLLLVRLTDHRL
                     QLTRQLQAPLQRHLNRYLQLLFPEASLSVDEALVPEILVRQGHGGEERGRFAELSFGA
                     REQMGLISRLAYADLLREAGRPTLVILDDALVHSDVERLGNMKRVLFDAADRHQILLF
                     SCHPDSWRDLGVVPRDVAALGG"
     gene            5048..5170
                     /locus_tag="PKN25_13850"
     CDS             5048..5170
                     /locus_tag="PKN25_13850"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92030.1"
                     /translation="MHRTPWARAQVEALYLDTRPPATGPSAGAQIHGPGAGIDS"
BASE COUNT          817 a         1790 c         2011 g          734 t
ORIGIN      
        1 gtcggcgatc tcctcgaggc tcaccgagtg cggcaagggg tagcggtgcc attccggctc
       61 gatgcccagc tgccggagga cctcgaccgc gtgctctccg agtgcgggag agacgacatc
      121 gtcgtgggtg ccgtgcgcgg cgaagaccgg aacctgtcga ttcgcttcca cgaacccatc
      181 gaccagcagc ccggggctcg ggatgtaggt cgacagcgcg atgatgccgg ccagcggggt
      241 ggggtgggtg agcccggtca gataggccac ggcgcccccc tgcgagaaac cggcgaggat
      301 gacgcgctcg ccgggcacgc cgcggtcggc ctcgcgctgg atcagttggc ggacgagggt
      361 gcgcgattcg agcagtccgg cctcgtcgat ctggcggctg tgcggggcga gcgagacgat
      421 gtcgtaccag gcgcgcatga cgtagccggc gttgcaggtg accgcacgat agggggcgtg
      481 cggaaagagg aagcgcacgg cggggctgcg gggcagggcc agcgccggga cgaccggctc
      541 gaaatcgctg ccgtccgcgc ccaggccgtg catccagatg acgctgtagc ggggatcggg
      601 tccggtctcg agctcgatgg gcggggtgtc gaagacgagg cctggggagg aatggaggct
      661 caagcgggtc accttgtggg agagtggagc gtcggcgcgc gcggccgcgc tcgcaattgg
      721 gcacggaaat gcggatgggc acggaaaaac ggatcgaacg gatgaatcct gaacacctgg
      781 tgctgctgct cgagcgcgag atgcccttcg gcaagtacaa ggggcgggtg atcgcggatc
      841 tgccgggata ctacctcgcg tggttcgccc gcaagggttt cccgccaggg cagctgggcc
      901 agttgctcgc gctgatgcac gagctcgacc acaacgatct ggccgggctg ctcgagccgc
      961 tgcgcagggc gcggaggcag gctgccgcag ggccgggcgg cgtgtaacat gccggccggt
     1021 gacaaacagg gatgtgcatc ggtcatgcgc ccgggtccgg tgatcggagc cccgggacgg
     1081 cggccgtgca tgcacgactt tccgctggat caggggacgg accgatgccg cgctttcttc
     1141 ataccgccga ttggcagatc ggccgccagt acgcgcgctt cgcgcccgag gacgcgctcg
     1201 cgctggccga ggcgcgcttc tcggcggtgg agcgcctggc gggcatcgcg cggcaggagg
     1261 cggtggatgc ggtgctggtg gccggggatg tcttcgatgc gcagacggtg tccgatcgca
     1321 cgatccgccg gctgttcaac gccatggggg cgtatgcagg cccgtgggtg ctgatccccg
     1381 gcaaccacga cgcggcgctg gccgagagcg tgtggtcgcg cgcccgccgc ctcggggccg
     1441 tggcgcccaa tgtgcatctg gcgctgggcg cgcaggtgat ggcgttcccc gagcgcggtt
     1501 tcgtcgtgct gccggcgccg ctcacccagc gccagacccc ggccgacgag accgcgtggt
     1561 tcgatcacgc ggagacggcg cccggcttgg tccgcgtcgg cctcgcccat ggcagcgtcc
     1621 agggcgtgct cgccgaggac atcgactcgc ccaacccgat cgccgccgac cgcgccaccc
     1681 gcgccgcgct ggattacctc gcgctcggcg actggcacgg cacgcggcgg atcgatgcgc
     1741 gcacctggta tagcggcacg cccgagcagg accgattcaa ggccaacgat ccgggcaatg
     1801 tgctgctcgt ggacatcccc gcggtcgggg ccgagccgga agtggaggtc cgggcggtgg
     1861 gccggcaccc ctggatcgcg ctcgaggaaa agctggcggt ggcctcggat ctcgagggca
     1921 cgctcgcccg cctggaggcc ttgcccgagc tcgccgtggt cgacctgagc ctgtccggcg
     1981 agatccacct gctcggcagg cagcggctcg aagccgcgct ctcggtggcg gaggcgaggt
     2041 tccgcagcct gcgcgccgac ctctccgggc tgcggctgca gcccaccgag gatgatctcg
     2101 ccgccctgca cgcagacggc tacgtgggcc gggtgatcgc cgagctgcgc gaggagcagg
     2161 atggcgagaa cgcggaggtc gcgagcgagg cgctggcgat cctcgccgcg ctgctgctcg
     2221 aggcgcaggc ccagggagcc gcggcatgag gctgctgcgc ctgaaggtcg aacagctacg
     2281 ccgcttccgg cagcccgtag agatccgcga tctcgatccc ggcatcaacc tcttcaccgg
     2341 accgaacgag tccggcaaga gcaccctggt gcgcgcgatc cgcgccgcat tcttcgagcg
     2401 ctacaagtcc tccagcgtgg aggatctgca gccctggggc gattcggccg ccgcgcccac
     2461 ggtcgagctg gagttcgagt cgcagggcaa gcgctggaag ctggtcaaga gcttcctgaa
     2521 gcgcaagcgc tgcgatgtca gcgtagacgg cgtggggttc agcggcgagg aggcggagga
     2581 caagctcgcg gacctgctcg gcttcgagtt ccccgggcgc ggggccagcc gaccggagca
     2641 ctggggcatc cccggcctgc tgtggatcga gcagggcgcc gggcaggaga tccacaagcc
     2701 ggtcgagcat gcgggcagcc acctgaagtc cgtcctgggc gccagcctcg gcgaggtgac
     2761 cagcaccgcg ggggacgagc tgatcgcccg gatcacccgg gagcgcgcgc gcctgctgac
     2821 cgccaccggc cggcccaccg gcgacttcgc tgcggcgctc gaggcgcacg agcggctcgc
     2881 gcgcgagcac gccgatctgc aggggcggat ccggcagtat cgccagcagg tggatcggct
     2941 cgggcagctg cgcgaggagc aggcccgcga cgagtccgag cgcatctggg aggtctaccg
     3001 gcggcaggcc agggcggctg acctccggct cgccgaggtc gtctcctggc agcgcgagca
     3061 ggagcgcgac cagcaggcgc tgcaggcctg ccaggccggc atgcgcacga gcctcgatct
     3121 gctcggccgc ttcgaggcgc agcgcaagga cctgcacagg cgcgccgccg accgccaggc
     3181 ggccgaacag gcgctcgccg cgcaggatgc gcgcaggccg gccgtggagc gtgtgctcgg
     3241 cgatgcccgc aaggccttcg aagcggcccg ggcgacggtc aggctggctc gcgagcatca
     3301 gcaacgcaag gacctggtac gggagctggc gcaggtcgat ggcgagctcg ccggcctgga
     3361 tcaggcgctg gcgcgcgccc gcgagctgca cgagcggtgg ctgcggcagc gcgcgttgcg
     3421 ccagtccctg caggtcgacg ccaaggcgct caagcgcctg cgcaaggtgg aggaggagct
     3481 cgcccggctt gccatccgcc aggaggcggt cgccacccga ctgcaattcg acctgcgcga
     3541 ggcgggcgcg ctcatcttgg gcggcgagcc cttgagcggg cggggggagc gcctcctgct
     3601 ggagccgacc gagatcctcg tcgcgcagct gggaagcctg cgcatcctgc ccggcggcga
     3661 cgacatcgcc gatctggtgc gcacgcgcca ggggctggcg gacgagaggg cgagcctcct
     3721 gtccgcgctc ggcgcggact cgctggccgc ggcggatgcc cgccacgagc aatgccgcga
     3781 gctcgacgag tccatcaagc gcgacgcatc ggtgctcgag tcgctggcgc ccgagggcct
     3841 cgaggcgctg gcgggtcgaa agcagctcac gcaggcgcgc cgggaggatc tcggcaaacg
     3901 gatcggggcc cttcccgaac cgcaggaaga cagcctctcg gtgtccaggg ccgaggcgca
     3961 gctcgacgct gccgaggtgt ccctcaaggc ggccgaggcc gacgaggcgg gcttccgcag
     4021 tgcgctcctg ctcgccacgc aggcgctgga gagtgcccgg gccgagtggg gccgactcga
     4081 cggcgagctg cagggcgcgg atcgcaaggc gcgcgaaaag caggccgagg accagctgct
     4141 ggagctgcgt gccggcgaga accggctcga ccaggccctg cgagaacggc agaagcagat
     4201 cgatcaggcc aatcccggcg tcctgcgcca ggacgtcgaa cgcttcacgc gcagtgcgga
     4261 tgcggcggag aaggccgccg agcagcgccg cctcgaactc gagcgcctcc gggccagcct
     4321 ggaggcgctc ggggcggagg gcctggagga gcgcagcgcg gaactggacg tcgagctggc
     4381 gcgtgctgcg cgccgtagcg acgagctcac gcggcgtgcc cgcgcgctgg acctcctgct
     4441 cgtccgtctg acggatcatc gcctgcagct cacccgtcag ctgcaggcgc ccttgcagcg
     4501 ccacctgaac cgctatctgc aactgctgtt tccggaggcc agcctgagcg tggacgaggc
     4561 gctcgtgccg gagatcctgg tgcgccaggg ccacggcggg gaagagcgtg gccgattcgc
     4621 cgagctgagc ttcggcgcgc gcgagcagat gggcctgatc agtcgcctcg cctacgccga
     4681 cctgttgcgc gaggccggcc ggccgacgct cgtgatcctg gacgatgcgc tggtgcacag
     4741 cgacgtcgag cgcctcggca acatgaagcg ggtgctgttc gatgctgccg accggcatca
     4801 gatcctgctg ttcagttgcc acccggacag ctggcgggac ctgggcgtcg tgccccggga
     4861 cgtcgccgcg ctgggaggct gagcgaccac gccatgaccc gccgacggcc cgacaactcc
     4921 ttccacggtc tcacgctgga ggaggtcctc ttccgacgcg cagaccggct cggcttgccg
     4981 acgctcggcg gcgggtgagc atccgcggct tcgatcacga acccggcatc gcctccggcc
     5041 cgagttcctg caccgcacgc cgtgggcgcg cgcacaggtc gaggccctgt acctcgacac
     5101 ccggccgccc gccaccggcc cgagcgcggg cgcgcagatc cacggtccgg gcgccggaat
     5161 cgactcataa gaatcttcga ttcattttgt gttgacggat tttctcgggt ctctataatg
     5221 cgcggcttcc cgaacgcaac agcgaatcgg cgaaacagga aagcagctga attctttcct
     5281 tgctgcaaga cggcagatct ttaaaaactt ggacaaccga taagtgtggg tgcttggttg
     5341 ttgtggtcgg cg
//
LOCUS       DAOQBS010000164         5317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_15999_length_5317_cov_14.217978, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000164 DAOQBS010000000
VERSION     DAOQBS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15999_length_5317_cov_14.217978"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PKN25_13855"
     CDS             <1..1082
                     /locus_tag="PKN25_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV92031.1"
                     /translation="RSFKQKNECHGWVGIRLQLHPGAAPSDVVMHVRMLDNDNALQSE
                     ALGILGVNLIHGAFFHHDQPEWVVEGLADGLGSERIEVDLIHFSGPYFDEVENRLMNL
                     HLIRSWLTRAVVFNPQGEVVVPGELLYRKPVMVMRGSFKPVTLVNVDMMAAGLRQFSQ
                     LAAVDDKEIVSLAEITMNSLISGDNVDGADFLARIDLLASQGYTVMISDYVRFFRLRA
                     YLRRYTQKPIGIVLSVRDFAFLFDEKYYEGLEGGILEAFGKLFPDNTHVYVYPSRPRG
                     TDASATVTLDNVAVPANLRHLLAHLKENDKLVAVQPHDDTHLHIDIGEVLTGLRRGRG
                     EWETDVPEAVAQRIIEGRLLGYDTE"
     gene            1274..3988
                     /locus_tag="PKN25_13860"
     CDS             1274..3988
                     /locus_tag="PKN25_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNV92032.1"
                     /translation="MSPRTAQLSPIENAHARAADEVLAALDSSPTGLSADEAARRLAE
                     IGPNRLPEPPRDGVVKRFFKHFNDVLIYVLLGAAAITASLGHWIDTGVILGVVVINAI
                     IGFIQEGKAEEALAGIRKMLSLRAEACRDGHWSAVDADGLVPGDIVRLRSGDRVPADV
                     RLIEATNLRIEESALTGESVPADKNTAAVEAAAGVGDRFGMAYSGTLVAAGRGIGVAV
                     ATGPDTELGRIDRLIAEVQPLQTPLTRQMAQFSKILSVLIVGLALLMLGVGLAVHEQP
                     LAEVFLAAIGFAVAAIPEGLPAILTITLALGVQRMARRNAITRKLTAVEALGSVTVIC
                     SDKTGTLTKNEMTVRHVLTRGGFYEVSGTGYRPEGEITLEGKVASLAHHDHLHALIEV
                     VAVANDTHVAEEDGQWKVTGEPTEGALRTLAHKAGFDAGAHQRHDSIPFESANKFMAT
                     LDTAPCGQRRILLKGAPDRLLERCRHELGADGELVALDRAFWEERIDALSGEGLRVLA
                     AAARDVPDSKDALVIEDLQEDMVFLGLIGIIDPPRPEAIAAIRSCHEAGIRVKMITGD
                     HAGTARAIGIEMGLGTADALKVITGAEIEAASDEELQRIAHDCDLFARTSPEHKLRLV
                     EALQARGEVVAMTGDGVNDAPALKRADVGVAMGIKGTEATKEAAEIVLADDNFSTIER
                     AVEEGRTIYDNLRKAILFILPTNGAQGFVMLAAVVLGLTLPLTPVQILWVNMVVAVTL
                     ALALAFEPAEPGVMQRPPRRPGAPIMDGAMLWRVGFVSLLIGGATIVSFEYSLLQGME
                     LQAARTMAVNTLVVAQAWYLFNSRFLTASSLAPARLFTNPAAQVSVLVLALLQLGFVY
                     LPFMNTWFGTTPLALEYWLPPLAIGALVFLVIEAEKALQRRYGARKSG"
     gene            4275..5165
                     /locus_tag="PKN25_13865"
     CDS             4275..5165
                     /locus_tag="PKN25_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNV92033.1"
                     /translation="MSKHKPIDIAEFRVEGGKKVDLADWPTKVRAVYDSKSDYEERLD
                     AGTERLAELQEMLYAHDRYSLLVIFQAMDAAGKDGAIRHVMSGINPQGCQVFSFKHPS
                     PTELDHDFLWRTHVALPERGRIGIFNRSYYEEVLIVRVLPEILRGQQLPDETLAARDF
                     WGERFRSIVDAEAHLHRNGTRIVKIFLHLSKDEQRDRLIARIDEEDKNWKFDEGDIEQ
                     RKHWDKYMAAYADCLRATSTPDCPWYVVPADDKKNARLIVSQILVDTLAGLDLHYPET
                     SPARREKLLEIRRQLEAGEV"
BASE COUNT          862 a         1977 c         1710 g          768 t
ORIGIN      
        1 cgcgcagctt caagcagaag aacgaatgcc acggctgggt cggcatccgc ctgcagctgc
       61 acccgggcgc cgcgccgagc gacgtggtca tgcacgtgcg catgctcgac aacgacaacg
      121 cgctgcagtc cgaggcgctc ggcatcctcg gcgtcaacct catccacggc gccttcttcc
      181 accacgacca gcccgagtgg gtggtggaag gtctcgccga cgggctcggc tccgagcgca
      241 tcgaggtcga cctgatccac ttctccggcc cgtatttcga cgaagtggaa aaccgcctga
      301 tgaacctgca cctgatccgc agctggctca cgcgcgcggt ggtcttcaac ccgcagggcg
      361 aggtggtggt gccgggcgag ctcctctacc gcaagccggt gatggtgatg cgcggcagct
      421 tcaagccggt gaccctggtg aatgtggaca tgatggccgc cggcctgcgc cagttcagcc
      481 agctcgccgc ggtcgacgac aaggagatcg tctcgctcgc cgagatcacc atgaactcgc
      541 tgatcagcgg cgacaacgtg gacggcgccg acttcctcgc ccgcatcgat ctgctcgcct
      601 cgcagggcta cacggtgatg atctcggact atgtgcgctt cttccgcctg cgcgcctacc
      661 tgcgccgcta cacccagaag ccgatcggga tcgtgctgtc ggtgcgcgac ttcgccttcc
      721 tgttcgacga gaaatactac gaaggcctgg agggcggcat cctcgaggcc ttcggcaagc
      781 tcttcccgga caacacccac gtctatgtgt acccgtcccg cccgcgcggc accgacgcct
      841 cggccacggt gacgctcgac aacgtggccg tccccgccaa cctgcgccac ctgctcgccc
      901 acctcaagga aaacgacaag ctggtggcgg tgcagccgca cgacgacacc cacctgcaca
      961 tcgacatcgg cgaggtgctc accggcctgc gccgcggccg cggcgagtgg gagaccgacg
     1021 tgcccgaggc ggttgcgcaa cgcatcatcg aaggccgcct gctcggctac gataccgagt
     1081 gagcggccct gtgcacgccc ccacgactcc ggaccctgca gcacgcgggg tctttttttg
     1141 aacttcggcg ggagcgacgg tctgtattaa tgcagcgcaa catccgcgcc gcaggcttgc
     1201 acaagcctct tgctttcgat cgcgcgcgcg gcatcctgcg cacagcgcat ccgcaccggc
     1261 acggagcacg gacatgagcc cgcgaaccgc ccagctttcc cccatcgaga acgcccacgc
     1321 ccgcgcggcc gacgaggtcc tcgcagcgct ggacagcagc ccgacgggcc tctctgcgga
     1381 cgaggccgca cgacgtctcg ccgagatcgg ccccaaccgc ctgcccgagc cgccccggga
     1441 cggcgtggtc aagcgcttct tcaagcattt caacgacgtg ctgatctacg tgctgctcgg
     1501 cgccgccgcg atcacggcga gcctcggcca ctggatcgac accggcgtca tcctcggcgt
     1561 ggtggtcatc aacgccatca tcggcttcat tcaggaaggc aaggccgagg aggcgcttgc
     1621 aggcatccgc aagatgctgt ccctgcgtgc cgaggcctgc cgcgacggac actggagcgc
     1681 ggtggacgcc gacggcctgg tgcccggcga catcgtgcgc ctgcgctcgg gcgaccgcgt
     1741 gcccgccgac gtgcgcctga tcgaggccac caacctgcgc atcgaggagt ccgcgctcac
     1801 cggcgagtcg gttcccgccg acaagaacac cgccgcggtc gaggccgccg ccggcgtcgg
     1861 cgaccgcttc ggcatggcct attcgggcac cctggtcgcc gccgggcggg gcatcggcgt
     1921 ggccgtcgcc accgggcccg acaccgagct cggccgcatc gaccgcctga tcgccgaggt
     1981 gcagcccctg cagaccccgc tcacccggca gatggcgcag ttcagcaaga tcctgtcggt
     2041 gctcatcgtc ggcctggcgc tgctcatgct gggcgtcggc ctcgcagtgc acgagcagcc
     2101 gctggccgag gtcttcctcg ccgcgatcgg cttcgcggtc gcggccatcc ccgaaggcct
     2161 gcccgccatc ctcaccatca cgctcgcgct cggcgtacag cgcatggcgc ggcgcaacgc
     2221 gatcacgcgc aagctcaccg cggtcgaggc gctcggctcg gtcacggtga tctgctcgga
     2281 caagaccggc acgctgacga agaacgagat gaccgtgcgc cacgtcctca cccgcggcgg
     2341 cttctacgag gtcagcggca ccggctaccg ccccgagggc gagatcaccc tcgaaggcaa
     2401 ggtcgccagc ctggcgcacc acgaccacct gcacgcgctg atcgaggtcg tcgcggtcgc
     2461 caacgacacc cacgtcgccg aagaggacgg ccagtggaag gtcaccggcg agcccaccga
     2521 gggcgcgctg cgcacgctcg cgcacaaggc cggcttcgac gccggcgcgc accagcgcca
     2581 cgacagcatc cccttcgagt cggccaacaa gttcatggcc acgctcgaca ccgccccctg
     2641 cgggcagcgc cgcatcctgc tgaaaggcgc gcccgaccgc ctgctcgagc gctgccgcca
     2701 cgagctcggc gccgacggcg agctcgtggc gctcgaccgc gccttctggg aggagcgcat
     2761 cgacgcgctg agcggcgaag gcctgcgcgt gctcgccgcc gccgcgcgcg acgttcccga
     2821 cagcaaggac gcgctcgtca tcgaggacct gcaggaggac atggtcttcc tcggcctcat
     2881 cggcatcatc gacccgccgc gcccggaagc gatcgcggcg atccgctcct gccacgaagc
     2941 cggcatccgc gtgaagatga tcaccggcga ccacgccggc accgcgcgcg caatcggcat
     3001 cgagatgggc ctgggcacgg ccgacgcgct gaaggtgatc accggcgccg agatcgaggc
     3061 cgccagcgac gaggaactgc agcggatcgc ccacgactgc gacctcttcg cgcgcaccag
     3121 ccccgagcac aagctgcgcc tggtcgaggc cctgcaggcg cgcggcgagg tggtggcgat
     3181 gaccggcgac ggggtcaacg acgcccccgc gctcaagcgc gccgacgtcg gcgtggcgat
     3241 gggcatcaag ggcaccgagg ccaccaagga ggccgccgag atcgtgctcg ccgacgacaa
     3301 cttctccacc atcgagcgcg ccgtcgagga aggtcgcacg atctacgaca acctgcgcaa
     3361 ggccatcctc ttcatcctgc ccaccaacgg cgcccagggc ttcgtgatgc tcgccgcggt
     3421 ggtgctcggc ctcaccctgc cgctgacccc ggtgcagatc ctgtgggtga acatggtggt
     3481 ggcggtcacg ctggcgcttg cgctcgcctt cgagcccgcc gagccgggcg tcatgcagcg
     3541 cccgccgcgg cgccccggcg cgccgatcat ggacggcgcg atgctgtggc gggtgggctt
     3601 cgtgtcgctg ctgatcggcg gcgccaccat cgtctcgttc gagtattcgc tgctgcaggg
     3661 catggaactg caggccgcgc gcacgatggc ggtcaacacc ctggtggtcg cgcaggcctg
     3721 gtacctcttc aacagccgct tcctcaccgc ctccagcctc gcgcccgcgc gcctgttcac
     3781 caacccggcg gcgcaggtct cggtgctggt cctggccctg ctgcagctcg gcttcgtcta
     3841 cctgcccttc atgaacacct ggttcggcac caccccgctc gcgctcgaat actggctgcc
     3901 cccgctcgcg atcggcgcgc tggtcttcct cgtgatcgag gccgagaagg cgctgcagcg
     3961 gcgatacggg gcgcgcaaga gcggctgagc gggcgcctcg ccaggcgccg atcgcgactt
     4021 gcgtcgctcc tgcagaaccg acacatcctc ggatcgcctg caggagcgac gcaagcgact
     4081 tcgccgcgtg ccgcgttcga cttctcccgc gcggggctcc tctgcgggtg gtcgcgccga
     4141 gcctccccgt gcagctccgg tgcgggccgc tcgcgccacg actcgcgcgc atccggtgca
     4201 tgacgtcggc cgcaaggagg cggacaatgt cccgcacgcc cgcttcggcc caccccaacc
     4261 agacggagca tccgatgagc aagcacaaac cgatcgacat cgccgagttt cgcgtcgagg
     4321 gcggcaagaa ggtcgacctc gccgactggc cgaccaaggt ccgcgccgtc tacgactcga
     4381 agagcgacta cgaggagcgc ctggacgccg gcaccgagcg cctggccgag ctgcaggaga
     4441 tgctctacgc ccacgaccgc tactcgctgc tggtgatctt ccaggccatg gacgcggccg
     4501 gcaaggacgg cgccatccgc cacgtcatgt cgggcatcaa cccgcagggc tgccaggtgt
     4561 tcagcttcaa gcacccgagc cccaccgagc tcgaccacga cttcctgtgg cgcacccacg
     4621 tcgcgctgcc cgagcgcggc cgcatcggca tcttcaaccg ctcgtactac gaggaggtgc
     4681 tgatcgtgcg cgtgctcccc gagatcctgc gcggccagca gctgcccgac gagaccctgg
     4741 ccgcgcgcga cttctggggc gaacgcttcc gctccatcgt cgacgccgag gcccacctgc
     4801 accgcaacgg cacgcgcatc gtcaagattt tcctgcacct ttcgaaggac gagcagcgcg
     4861 accgcctgat cgcccgcatc gacgaggaag acaagaactg gaagttcgac gaaggcgaca
     4921 tcgagcagcg caagcactgg gacaagtaca tggccgccta cgccgactgc ctgcgcgcca
     4981 ccagcacgcc cgattgccca tggtacgtgg tgccggccga cgacaagaag aacgcccgcc
     5041 tgatcgtctc gcagatcctg gtcgacaccc tcgcggggct cgacctgcac taccccgaga
     5101 ccagtccggc acggcgcgag aagctcctcg agatccgccg ccagctcgaa gccggcgagg
     5161 tctgacttcg ccggcctccg gatgcaccac gacgtcttgt gggagcgacg cgttgtgctg
     5221 cgttccggcc gcgttaccct ttgacgcgtc cggatgcacc acgacgtctt gtgggagcga
     5281 cgcgagtcgc gattgcagcc tgcgggagtc gtggcgc
//
LOCUS       DAOQBS010000165         5302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_16076_length_5302_cov_17.821803, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000165 DAOQBS010000000
VERSION     DAOQBS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16076_length_5302_cov_17.821803"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(558..1205)
                     /locus_tag="PKN25_13870"
     CDS             complement(558..1205)
                     /locus_tag="PKN25_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNV92034.1"
                     /translation="MPTIRATLHATLAASLLLALLPPAHAQRAGNIGGCDIRRGTLCT
                     NLNLNGANLEGVNLANSQFTRSDLSGANLRGATLNEANFSQAEMAGATLDKASMLRAN
                     LRGARLTGASFKEADLRNAILQNADLHDADLTAAQLGGADLTNARAERSRFDGAELTR
                     SNLRAAKLAGASFVGANLQGANFTRAQLSNADFSGANLDQAVFLNAQTDGCKGCP"
     gene            complement(1226..1723)
                     /locus_tag="PKN25_13875"
     CDS             complement(1226..1723)
                     /locus_tag="PKN25_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNV92035.1"
                     /translation="MKLKIAAAVAVLVLAVAGFFVFTQKPAAPDVAFSTLQGQSFRTA
                     DLRGKVVLVNFWATTCTTCIKEMPALKATQEKFEARGYRTVAVAMDYDPPAQVAAFVE
                     RSALPFTFVLDRDGSIARGFEGVRLTPTSYVLDKHGRIVQKILGEPDFDKLHGLLEQL
                     LAEPA"
     gene            1926..2366
                     /locus_tag="PKN25_13880"
     CDS             1926..2366
                     /locus_tag="PKN25_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV92036.1"
                     /translation="MTSPPVFTWSDRFLLGHRTIDDTHREFVECVDALLRASDAEIPA
                     ALDAFARHAEAHFAQEDAWLSAADFPTGGDCHIDEHAKVMASVREVQELVAKGEVEIA
                     RELAQALMDWFPGHADYMDSALATWLVKKSHDGRPLVLRRKENL"
     gene            2528..4519
                     /locus_tag="PKN25_13885"
     CDS             2528..4519
                     /locus_tag="PKN25_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNV92037.1"
                     /translation="MSALLNAPQREAIRYLDGPCLVLAGAGSGKTRVITHKIAHLINE
                     CGIGAANIAAITFTNKAAKEMQERVAHIMGGRVPGGLTVCTFHALGVRIIRQEAKHCG
                     LKPQFSILDASDTVQIVSDVAGDSDKGIAKQMQWQISSWKNAMITPEEAAQLADNEIA
                     SVAARLYKEYERTLRAYQAVDFDDLISLPVRLFDEHPEVRERWQNKLRYLLVDEYQDT
                     NRAQYRLLKLLSGVRGAFTAVGDDDQAIYAWRGADVENLKLLQQDYPKLKVIKLEQNY
                     RSSRRILEAANTVIANNAKLFDKRLWSEHGQGEQIVVTNCRDAEHEAEWVATKITAHK
                     FEHRTRFKDYAILYRGNHQARLIEQQLRNHRIPYVMSGGQSFFDKAEIRDLIAWLRLL
                     VNEDDDLAFIRAITTPRRGIGAATIEALGAYAGHRHTSLFTAVFEEGLTQHLNAKQLQ
                     GVQEFAAYINRLQYRAPREPAAQLLEDLLGAIRYEAWLFEHCDTREAESKWSNVRDFV
                     GWLGRKGEEDGKNLLELTQTIALMSMLDKEDPDFDGVQMATLHASKGLEFPHVFLVGV
                     EEGLLPHQSSIDEDKVEEERRLMYVGITRAQRSLNLTWCERRKSGKEFRTCEPSRFIA
                     EMGGDIKMNDRKTAQPVTKEEGKARLANLMAMFENRDKA"
     gene            complement(4658..5104)
                     /locus_tag="PKN25_13890"
     CDS             complement(4658..5104)
                     /locus_tag="PKN25_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV92038.1"
                     /translation="MSNPRPLRPARLAMPVALVAAALLAGCGDSGKVDEELRATLIQP
                     VARVEIQAVTITPGTRTGEQIYKSICAACHDAGAVGAPKTGDAAAWGPRLALGHDGLT
                     ASAIAGKNAMPPRGGGSDLTDTEVKRAVAYLANLAGAGYTEPPVEQ"
BASE COUNT          830 a         1820 c         1865 g          787 t
ORIGIN      
        1 tctgagaaga ctcccgcggc tggccgggcg acggcttcgc gctgacgcag gtcggaccgg
       61 ggcgccaacc cccgcgggtg gcgtggcccc ggggttttcg ctggctcggg gcgtcgcggc
      121 ttcgctccgc cccggggctc gctgcgccgg ggctttcgcg gctccggtcc ggccttgggg
      181 ccggggtggc gctcgccgcc tgcactcgcg gcccctcgct tcgctcgggg ctgccctcgg
      241 cggcggacgc cgcgggggcg gcgacgcaaa ctcgtcgctg cgctcctcgg acatgcgtcg
      301 ccttgtttcc cccgcgacgc cctccgcctc ggcgctcctg aagttggcga gcgccacccc
      361 ggccccaagg cctcgcgcct gcgcgcatca gggcgccgaa aaacctcgcc gatcagaagt
      421 catcacgctt ccgaagccgc gccgtaggag cgacgcaagt cgcgatcgcg gccgccggtc
      481 gaccgccgcg cgcgttcccg gcgccgccgt cgccgtggat tcccgcgtcg atccgtcccg
      541 acctgcgaga accccgccta cggacagccc ttgcacccgt ccgtctgcgc gttgaggaag
      601 accgcctgat ccaggttcgc accgctgaag tcggcgttgc tcagttgcgc gcgggtgaag
      661 ttcgccccct gcaggttggc gccgacaaag gacgcgcccg cgagcttggc ggcgcgcagg
      721 ttgctgcggg tgagctcggc gccatcgaag cggctgcgct cggcgcgggc gttggtcagg
      781 tcggcgccgc cgagctgggc ggcggtgaga tcggcgtcgt gcaggtcggc gttctgcagg
      841 atggcgttgc gcaggtcggc ttccttgaag ctggcgccgg tcaggcgtgc gccacgcagg
      901 ttggcgcgca gcatcgaggc cttgtccagc gtcgcgcctg ccatctcggc ctgggagaag
      961 ttggcttcgt tcaaggtggc gccgcgcagg ttggcgccgg acagatccga gcgggtgaac
     1021 tgcgagttgg ccagattcac cccttcgagg tttgccccgt tgaggttgag gttggtgcac
     1081 agcgtgccgc ggcggatgtc gcagccgccg atgttgccgg cgcgctgggc gtgggcgggc
     1141 gggagcaggg cgagcagcag gctggccgcg agggtggcgt gcagtgtggc gcggatcgtg
     1201 ggcatggggt gcgtgggctt cccggtcagg cgggttcggc gagaagctgt tcgagcaggc
     1261 cgtgcagctt gtcgaagtcg ggttcgccga ggatcttctg cacgatcctg ccgtgcttgt
     1321 cgaggacgta gctggtcggg gtcaggcgca cgccctcgaa gccgcgcgcg atgctgccgt
     1381 cgcggtcgag cacgaaggtg aagggcaggg cgctgcgctc gacgaaggcg gcgacctggg
     1441 cgggcgggtc gtagtccatc gccaccgcca cggtgcggta gccgcgcgcc tcgaacttct
     1501 cctgcgtcgc cttcagcgcc ggcatctcct tgatgcaggt ggtgcaggtc gtggcccaga
     1561 agttcaccag cactaccttg ccgcgcaggt cggcagtgcg gaagctctgc ccctgcagcg
     1621 tggagaaggc cacgtcgggg gcggcgggct tctgggtgaa cacgaagaag ccggcgactg
     1681 cgaggacgag gaccgcgacg gcggcggcga tcttgagctt catggcgggg tccggaacgg
     1741 ttgaactgcg cggcggcagg cgctttacgg gcgattttga gcgagcgccc ggggcttgtc
     1801 accttcgacc ggtcggacag gcgctgcctg caaaggtgcc cgtcttgcta gacttcaagc
     1861 atcggggacg cgcgaccggc gtccctgcac ccgttttccg acattcgatc cgaggcaaga
     1921 ccgcaatgac cagccccccc gtgtttacct ggagtgaccg attcctgctc gggcaccgca
     1981 cgatcgacga cacccaccgc gagttcgtcg agtgcgtgga tgcgctgctg cgcgcgagcg
     2041 acgcggagat ccccgccgcg ctcgacgcct tcgcccgcca cgccgaggcg cacttcgcgc
     2101 aggaggacgc ctggctctcc gcggccgatt tccccaccgg tggcgactgc cacatcgacg
     2161 agcacgccaa ggtgatggcg tcggtgcgcg aggtgcagga gctcgtcgcc aagggcgagg
     2221 tggagatcgc gcgcgagctc gcgcaggcgc tgatggactg gttccccggc cacgccgact
     2281 acatggactc ggcactcgcc acctggctgg tgaagaagtc ccacgatggg cgtccgctgg
     2341 tgctgcggcg caaggaaaac ctgtgagcgc ctcgccaggc gggcgcaggc gacctcgggc
     2401 gagcgcccgg tgggcgcgca tccggcgccg tccggccggc actcgccggg ccgcacgccc
     2461 gatcggctag aatcgatccc cacgccgccc ggccgggcgg ctttgctttt ctgcgatcca
     2521 gctcacgatg tccgccctcc tcaacgcgcc ccagcgcgaa gcgatccgct atctcgacgg
     2581 cccctgcctg gtgctggccg gcgccggcag cggcaagacg cgggtgatca cgcacaagat
     2641 cgcgcacctg atcaacgagt gcggcatcgg cgcggccaac atcgccgcga tcaccttcac
     2701 caacaaggcc gccaaggaga tgcaggagcg cgtagcccac atcatgggcg ggcgcgtgcc
     2761 gggcgggctc accgtgtgca ccttccacgc gctcggcgtg cgcatcatcc gccaggaggc
     2821 caagcactgc ggcttgaagc cgcagttctc cattctggac gcctccgaca ccgtgcagat
     2881 cgtctcggac gtggccggcg acagcgacaa gggcatcgcc aagcagatgc agtggcagat
     2941 ctcgtcgtgg aagaacgcga tgatcacgcc cgaggaggcc gcgcagctcg ccgacaacga
     3001 gatcgcctcg gtcgccgcca ggctctacaa ggaatacgag cgcaccctgc gcgcctacca
     3061 ggcagtggat ttcgacgacc tcatctcgct gccggtgcgc ctgttcgacg agcaccccga
     3121 ggtgcgcgag cgctggcaga acaagctgcg ctacctgctg gtggacgagt accaggacac
     3181 caaccgcgcc cagtaccggc tgctcaagct gctgtcgggc gtgcgcggcg ccttcaccgc
     3241 ggtgggcgac gacgaccagg cgatctacgc ctggcgcggc gccgacgtcg agaacctgaa
     3301 gctgctgcag caggactacc ccaagctgaa ggtgatcaag ctcgagcaga actaccgctc
     3361 ctcacgccgc atcctcgaag ccgccaacac cgtcatcgcc aacaacgcga agctcttcga
     3421 caagcgcctg tggtccgagc acggccaggg cgagcagatc gtcgtcacca actgccgcga
     3481 cgccgagcac gaggccgagt gggtggcgac caagatcacc gcgcacaagt tcgagcaccg
     3541 cacccgcttc aaggactacg ccatcctcta ccgcggcaac caccaggccc gcctcatcga
     3601 gcagcagctg cgcaaccacc gcatccccta cgtgatgtcg ggcgggcaga gcttcttcga
     3661 caaggccgag atccgcgacc tcatcgcctg gctgcgcctg ctggtcaacg aggacgacga
     3721 cctcgccttc atccgcgcca tcaccacgcc gcgccgcggc atcggcgccg ccaccatcga
     3781 ggcgctcggc gcctacgccg ggcaccgcca caccagcctg ttcaccgcgg tgttcgagga
     3841 aggcctcacc cagcacctca acgccaagca gctgcagggc gtgcaggagt tcgccgccta
     3901 catcaaccgt ctgcagtacc gcgccccgcg cgagcccgcc gcgcagctgc tggaagacct
     3961 gctcggcgcg atccgctacg aggcctggct gttcgagcac tgcgacacgc gcgaggccga
     4021 gtcgaagtgg agcaacgtgc gcgatttcgt cggctggctt ggccgcaagg gcgaggagga
     4081 cggcaagaac ctgctcgagc tcacccagac catcgcgctg atgtccatgc tcgacaagga
     4141 agaccccgac ttcgacggcg tgcagatggc caccctgcac gcctccaagg gcctggagtt
     4201 tccgcatgtg ttcctggtcg gcgtcgagga gggcctgctg ccgcaccaga gcagcatcga
     4261 cgaggacaag gtcgaggaag agcgccgcct gatgtacgtc ggcatcaccc gcgcgcaacg
     4321 cagcctcaac ctgacctggt gcgagcggcg caagtcgggc aaggagttcc gcacctgcga
     4381 gccgagccgc ttcatcgccg agatgggcgg cgacatcaag atgaacgacc gcaagaccgc
     4441 acagccggtg acgaaggaag aaggcaaggc gcggctggcg aacctgatgg cgatgttcga
     4501 gaaccgcgac aaggcttgag cggccgctcg cttgtggcgc cccgacagga aagggcgcga
     4561 taggggtctg ctgcaggccg gggccccagg cgcaaaaaag gcagccgcgg aggctgcctt
     4621 tcctgacctg gccgacggct gcgacgcagc ccgcggatta ctgctcgacc ggcggctcgg
     4681 tatagccggc gccggcgagg ttcgcgaggt aggccacggc gcgtttcacc tcggtgtcgg
     4741 tgaggtcgct gccgccaccg cgcgggggca tggcgttctt gccggcgatc gccgaggcgg
     4801 tcaggccgtc gtggccgagc gccaggcggg gtccccaggc cgcggcgtcg ccggtcttcg
     4861 gcgcacccac cgcgccggcg tcgtggcagg cggcgcagat cgacttgtag atctgctcgc
     4921 cggtgcgggt tcccggcgtg atggtcacgg cctggatctc gacacgggcg accggctgga
     4981 tcagcgtggc gcggagttct tcatcgacct tgcccgagtc gccacagccg gccagcagcg
     5041 cggctgcgac cagggcgacg ggcattgcga ggcgggcggg ccgcaggggg cgtgggttcg
     5101 acatgctttg atcctcggac ggtggcgcaa gacagggtct aaagccggga ttatagcggg
     5161 ctaaaaacca ttgtggcagc gcgccatgga ctttgccgcc gaagcgggct atgcttccgc
     5221 gccttgtccg ggcgcttcgt ccggacacca cgcgcccgta gctcagctgg atagagtact
     5281 gccctccgaa ggcaggggtc gg
//
LOCUS       DAOQBS010000166         5297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_16099_length_5297_cov_15.998665, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000166 DAOQBS010000000
VERSION     DAOQBS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16099_length_5297_cov_15.998665"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3505)
                     /locus_tag="PKN25_13895"
     CDS             complement(<1..3505)
                     /locus_tag="PKN25_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNV92039.1"
                     /translation="MDLPQKQGLYDPANEHDACGVGFIAHIKGEKRHDIILQGLEILK
                     NLDHRGAVGADPLQGDGAGILIQIPDQLYREEMAAQGVTLPLPGEYGVGMVFMPKEAA
                     SRLACEQEIERAVVAEGQVVLGWRDVPVNRDMPMSPTVKAMEPMIRQIFIGRGPDVMV
                     TEALERKLYVVRRRSANAIGALKLKHAKEFYMVSCSARTVNYKGLLLADQVGEYYPDL
                     RDARAVSALSLVHQRFSTNTFPKWNLAHPFRYIAHNGEINTLRGNYNWMRAREKGVHS
                     PLLGDDLQKIWPLIYPGQSDSASFDNALELLVMSGYSLAHAMMMMIPEAWESHTQMDE
                     KRRAFYEYHAAMMEPWDGPAAVAFTDGRQIGATLDRNGLRPARYLITDDDLVVMASES
                     GVLPIADSKIVKKWRLQPGKMFLIDMEQGRIIGDQEIKESLAHARPYADWLRRINIKL
                     DTLEAPAVLDTAAADERVAPLLDRQQAFGYTQEDIKFILEPMGKAGEEGTGSMGNDSP
                     LAVLSSKNKPLFNYFRQLFAQVTNPPIDPIREQMVMSLVSFIGPRPNLLEINEINPPF
                     RLEVSQPVLDFEGMAKIRNIARYTQNKFRSAELDICYPAEWGNEGVEARLASLCADAE
                     NAVLAGTNILIVSDRKLAADRVAIPALLALSAIHQHLVEKGLRTRTGLVVETGSAREV
                     HHFAVLAGYGAEAVHPYLALETLAHLADDAETAAKYVKHFVKAIGKGLMKVMSKMGIS
                     TYMSYTGAQIFEAVGLKQALLDKYFTGTTSQVEGIGVFEVMEESIRLHKAAFGTDPVL
                     HDMLDAGGEYAFRIRGEEHMWTPDAIAKLQHATRSGKADTYKEYAKIINDQSRRHMTL
                     RGLFELKAALTPVPLDEVEPAKEIVKRFATGAMSLGSISTEAHTTLAVAMNRIGGKSN
                     TGEGGEDPMRFKPLTEAIKLSQVIGEKRIARDLDLNAGDSLRSAIKQVASGRFGVTTE
                     YLVNADQIQIKMAQGAKPGEGGQLPGHKVSEYIGFLRHSVPGVGLISPPPHHDIYSIE
                     DLAQLIHDLKNTNPAASISVKLVSEIGIGTVAAGVAKAKADHIVVAGHDGGTGASPWS
                     SIKHAGSPWELGLAETQQTLVLNRLRGRVRLQVDGQIKTGRDVVIGALLGADEFGFAT
                     APLVVEGCIMMRKCHLNTCPVG"
     assembly_gap    4065..4164
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4277..>5297)
                     /locus_tag="PKN25_13900"
     CDS             complement(4277..>5297)
                     /locus_tag="PKN25_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNV92040.1"
                     /translation="RIVHSTAFRRLEYKTQVFVNHEGDLFRTRLTHSIEVAQLTRGIA
                     RELGLNEDLAEAIALAHDLGHTPFGHAGQDALNACMKDYGGFEHNLQSLRTVDLLEDR
                     YAGFDGLNLMFETREGILKHCSRANAERLGELGQRFLDGTQPSLEAQLANLADEIAYN
                     NHDVDDGLRSGLITLEQLDEVPIFAVQRREAEARWSGLSGRKLINETVRRMIHLMVID
                     LIEQTRANIAAEGVATLADVHAAPRLVAYSDALLPRLRELKVFLRDKLYRHYQVLRMT
                     NKARRIVGDLFAAFMDDPHILPPQYQAMAREDKPRAIADYIAGMTDRYAMKEHRRLFA
                     VGEIY"
BASE COUNT          796 a         1668 c         1820 g          913 t
ORIGIN      
        1 cgccgaccgg gcaggtgttg aggtggcact tgcgcatcat gatgcagccc tcgacgacga
       61 gcggcgcggt ggcgaagccg aactcgtcgg cgccgagcag cgcgccgatg acgacgtcgc
      121 ggccggtctt gatctggccg tcgacctgca ggcgcacgcg tccgcgcagg cggttgagca
      181 ccagggtctg ctgggtctcg gccagcccga gctcccacgg cgagccggcg tgcttgatcg
      241 agctccacgg gctcgcaccg gtgccgccgt catggccggc aaccacgatg tggtcggcct
      301 tggccttggc cacgcccgcc gccaccgttc cgatgccgat ctcggacacc agcttgaccg
      361 agatgctcgc ggccgggttg gtgttcttga ggtcgtggat gagctgcgcg aggtcctcga
      421 tggagtagat gtcgtggtgc ggcggcggcg agatgaggcc aacgcccggc accgagtgac
      481 gcaggaagcc gatgtactcc gagaccttgt ggccgggcag ctggccgccc tcgccgggct
      541 tggcgccctg ggccatcttg atctggatct ggtcggcgtt gaccaggtac tcggtggtga
      601 cgccgaagcg gcccgaggcc acctgcttga tcgccgagcg cagcgagtcg ccggcgttga
      661 ggtcgaggtc gcgcgcgatg cgcttctcgc cgatcacctg cgacagcttg atcgcctcgg
      721 tcagcggctt gaaacgcatc gggtcttcgc cgccctcgcc ggtgttcgac ttgccgccga
      781 tgcggttcat cgccaccgca agcgtggtgt gcgcctcggt ggagatcgag ccgagcgaca
      841 tcgcgccggt ggcgaagcgc ttgacgatct ccttggcggg ctcgacctcg tcgagcggca
      901 ccggggtgag cgcggccttg agctcgaaca ggccgcgcag cgtcatgtgg cggcggctct
      961 ggtcgttgat gatcttggcg tattccttgt aggtgtcggc cttgcccgag cgcgtggcgt
     1021 gctgcagctt ggcgatcgcg tccggcgtcc acatgtgctc ctcgccgcgg atgcggaagg
     1081 catactcgcc gccggcgtcg agcatgtcgt gcagcaccgg gtcggtgccg aaggcggcct
     1141 tgtgcaggcg gatggattct tccatcacct cgaacacgcc gatgccctcg acctggctgg
     1201 tggtgccggt gaagtacttg tcgagcagcg cctgcttgag gccgacggct tcgaagatct
     1261 gcgcgccggt gtaggacatg taggtcgaga tgcccatctt ggacatgacc ttcatcaggc
     1321 ccttgccgat cgccttgacg aagtgcttga cgtacttggc cgcggtctcg gcgtcgtcgg
     1381 ccaggtgggc cagggtctcg agcgcgaggt aggggtgcac ggcttcggcg ccgtagccgg
     1441 cgagcaccgc gaagtgatgc acctcgcgcg ccgagccggt ctcgaccacc aggccggtgc
     1501 gggtgcgcag gcccttctcc accaggtgct ggtggatcgc ggacagcgcc agcagcgcgg
     1561 ggatggcgac gcggtcggcg gcgagcttgc ggtcggacac gatgaggatg ttggtgcccg
     1621 ccagcaccgc gttctcggcg tcggcacaca gcgaggccag gcgcgcctcg acgccctcgt
     1681 tgccccactc cgccgggtag cagatgtcca gctcggcgga gcggaacttg ttctgcgtgt
     1741 agcgcgcgat gttgcggatc ttggccatgc cctcgaagtc gagcacgggc tggctcacct
     1801 cgaggcggaa gggcgggttg atctcgttga tctcgagcag gttggggcgc gggccgatga
     1861 aggacaccag cgacatcacc atctgctcgc ggatcggatc gatcggcggg ttggtgacct
     1921 gcgcgaagag ctggcggaag tagttgaaca gcggcttgtt cttcgaggac agcaccgcca
     1981 gcggcgagtc gttgcccatc gagccggtgc cttcctcacc tgccttgccc atcggctcga
     2041 ggatgaactt gatgtcctcc tgcgtgtagc cgaaggcctg ctggcgatcg agcagcggcg
     2101 cgacgcgctc gtcggcggcg gcggtgtcga gcacggccgg cgcctcgagg gtgtcgagct
     2161 tgatgttgat gcggcgcagc cagtcggcgt agggcctggc gtgggcgagc gactccttga
     2221 tctcctggtc gccgatgatg cggccctgct ccatgtcgat caggaacatc ttgccgggct
     2281 gcaggcgcca cttcttgacg atcttgctgt cggcgatggg cagcacgccg gactcggagg
     2341 ccatcaccac gaggtcgtcg tcggtgatca ggtagcgcgc cggacgcagg ccgttgcggt
     2401 cgagggtggc accgatctgg cggccgtcgg taaaggccac cgccgcgggg ccgtcccagg
     2461 gctccatcat cgccgcgtgg tactcgtaga aggcgcggcg cttctcgtcc atctgcgtgt
     2521 gcgactccca ggcctcgggg atcatcatca tcatcgcgtg ggcgagcgag tagccgctca
     2581 tcaccagcag ctcgagcgcg ttgtcgaagg aggccgagtc ggactggccg gggtagatca
     2641 gcggccagat cttctgcagg tcgtcaccga gcagcggcga atgcacgccc ttctcgcgcg
     2701 cgcgcatcca gttgtaattg ccgcgcaggg tgttgatctc gccgttgtgc gcgatgtagc
     2761 ggaacgggtg ggcgaggttc cacttcggga aggtgttggt cgagaagcgc tgatgcacca
     2821 gcgacagcgc cgacaccgca cgcgcatcgc gcaggtcggg gtagtactcg ccgacctggt
     2881 cggcgagcag caggcccttg tagttcacgg tgcgtgccga gcacgacacc atgtagaact
     2941 ccttggcgtg cttgagcttg agcgcgccga tggcgttggc ggagcggcgg cgcacgacgt
     3001 agagcttgcg ctcgagcgcc tcggtgacca tgacgtcggg accgcggccg atgaagatct
     3061 ggcggatcat cggctccatg gccttgaccg tgggcgacat cggcatgtcg cggttgaccg
     3121 gcacgtcgcg ccagccgagc acgacctggc cttcggccac gaccgcacgc tcgatctcct
     3181 gctcgcaggc gaggcgcgag gcggcttcct tgggcatgaa caccatgccg acgccgtact
     3241 cgccgggcaa cggcagggtc acgccctgcg ccgccatctc ctcgcggtag agctggtcgg
     3301 ggatctggat caggatgccc gcgccgtcgc cctgcagcgg gtcggccccc accgcgccgc
     3361 gatggtcgag gttcttcagg atctcgaggc cctgcagaat gatgtcatga cgtttctcgc
     3421 ccttgatgtg ggcgatgaaa cccacgccac aggcatcgtg ttcattggcg gggtcataca
     3481 gaccctgctt ttggggcaga tccatcttcg attcctcaaa cgacactcaa gatcacgtgc
     3541 tcggggtcgg acgaaatcgg ccgaaaggcc gtcgccgaca aaccgaacgc gcacaaaaaa
     3601 gccgggcgcg cgcgcaccgg ctttgtagtc tgcaccaatc tggtgcagaa acagcgattc
     3661 cacagggtcg aagggcgacc ttttcaggcc tcatgcacca ttttggacac taccgtcctg
     3721 ttcgctgcgg tcgaaaaact atacccccca aagctcgccg gattcaacag tttttttgca
     3781 ctgcggcacg cgacgaaaac acccgccggg cggcctctcc gagaccccgc agtcgccccc
     3841 cgcagccgaa tggcaccgcg acgctcggct tgcccgtggg agcgacgcga gtcgcgattc
     3901 ggcgaacccg atgtgcggcg cgcaaacgag cgccgcgggg gctcgcatgc atggatcgcg
     3961 actcgcgtcg ctcctacgaa aaccaccgcc caccccgccg ccgcgcccga tgcgcgccat
     4021 ggtgctcggc ttgcccgtgg gagcgacgcc agtcgcgatt cggcnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnatgcgc gccatggtgc tcggcttgcc cgtgggagcg
     4201 acgccagtcg cgattcggcc gacccgactc gccgtgcagt ccggagactg caccgagcat
     4261 gcaggaagcc cccgtctcaa taaatctccc ccaccgcgaa caggcgccgg tgctccttca
     4321 tcgcgtagcg gtcggtcatg ccggcgatgt agtcggcaat ggcgcgcggc ttgtcctcgc
     4381 gcgccattgc ctgatactgc ggcggcagga tgtggggatc gtccatgaac gccgcgaaca
     4441 ggtcaccgac gatgcggcgc gccttgttgg tcatgcgcag cacctggtag tggcggtaga
     4501 gcttgtcgcg cagaaagacc ttgagctcgc gcagacgcgg cagcagcgcg tcggaatacg
     4561 ccaccaggcg cggtgcggca tggacgtcgg cgagcgtcgc caccccctcg gcggcgatgt
     4621 tggcgcgagt ctgttcgatg aggtcgatca ccatcaggtg gatcatgcgc cgcaccgtct
     4681 cgttgatcag cttgcgcccc gaaagacccg accagcgcgc ctcggcctcg cgccgctgca
     4741 ccgcgaagat cggcacctcg tcgagctgct cgagcgtgat cagccccgag cgcaggccgt
     4801 catccacgtc gtgattgttg taggcgatct cgtcggcgag gttggcgagc tgggcctcga
     4861 gcgagggctg ggtgccgtcg aggaagcgct ggccgagctc gccgaggcgc tcggcgttgg
     4921 cacgcgagca gtgcttgagg atgccctcgc gcgtctcgaa catcaggttg agcccgtcga
     4981 agcctgcata gcggtcttcc agcaggtcca ccgttcgaag cgactgcagg ttgtgctcga
     5041 agccgccgta gtccttcatg caggcgttga gcgcgtcctg cccggcgtgg ccgaaggggg
     5101 tatggccgag gtcgtgcgcg agcgcgatgg cctcggcgag atcctcgttg aggccgagct
     5161 cgcgcgcgat gccgcgggtg agctgggcca cctcgatgct gtgcgtgagt cgggtgcgga
     5221 acagatcgcc ctcgtggttc acgaacacct gggtcttgta ttccaggcgg cggaaggcgg
     5281 tggaatgcac gatgcgg
//
LOCUS       DAOQBS010000167         5288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_16136_length_5288_cov_15.459010, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000167 DAOQBS010000000
VERSION     DAOQBS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16136_length_5288_cov_15.459010"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PKN25_13905"
     CDS             <1..864
                     /locus_tag="PKN25_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNV92041.1"
                     /translation="TAALADIVLPICTPWEREALRVGFEGSQAAESWVQLRPAAIAPL
                     GDSRADAWVVFRLAQRLGLGEQFWGGDLEAGLAHVLAPTGLTLETLRAHPGGLALPAA
                     TRYRKYRDEGLRTPSGRIELYSETLLEHGESPLPRFAEPVDSPFGKAAPEYPLVMTTA
                     KLRHFRHGQDRQAPSLRRHSPQPAVHLHPDAARARAIAEGDWVRVRTANGAIRLRARF
                     DAALDARVVWVEYGWWQPGAAGAADGYDARSEAGANYSRLVSDRRVDPVAGSQPLRSG
                     LCEIEPLSSSG"
     gene            complement(848..>2426)
                     /locus_tag="PKN25_13910"
     CDS             complement(848..>2426)
                     /locus_tag="PKN25_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor I family protein"
                     /protein_id="HNV92042.1"
                     /translation="SVGRLDSQALLAGSEINSVAVRDGVVAVAIQASPKTDKGRVALY
                     RAADLALLGSVEVGALPDMLTFTPDGKTVLVANEGEPNDDYSIDPEGSISVIDVSTPT
                     APVARSAAFTAFNGQEKALRAQGVRIYGPGASAAQDLEPEYIAVSADGATAWATLQEN
                     NALARIDVATATVEAILPLRDKDHGVDANAFDPSDTDKKAVIQAWPGVRGLYLPDAIA
                     SYTHQGRTLLVTANEGDARTWGEGDDAYWDGDASKGFVEEFRVKHLTNTKGWSGRKGD
                     DLPPQLDALADGGLLDPTVFAYCGAVAGAPGKCRDDDQLGRLNITWTMGYRQDADGKP
                     ILFDTAGNENPAGTRLMYDTLYAFGGRSFSIRDADGKLVWDSGAEIERFLASDECKLG
                     SARNIPCATWFNANHEEGDTLDNRSDNKGAEPEGVALGRIGDKTFAFVGLERMGGVLV
                     YDITDPQAPTRVDYLNPREDWSTKDPSTVLASVGDLGPEGLHFIPAKDSPNAKPLLLV
                     GNEVSGTTAVYQLNLSY"
     assembly_gap    2427..2526
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2527..2737)
                     /locus_tag="PKN25_13915"
     CDS             complement(<2527..2737)
                     /locus_tag="PKN25_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNV92043.1"
                     /translation="MSHRLRHALETFSLSLLAAATLAACTGDDDTPPPPPATETAPAP
                     QAPAPERTPISLRLERIGGYATNLFG"
     gene            complement(3008..4027)
                     /locus_tag="PKN25_13920"
     CDS             complement(3008..4027)
                     /locus_tag="PKN25_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNV92044.1"
                     /translation="MQNLKYFTWSIGFTVFGLVGGYLLGGWPAAFIVAVLAVLETSLS
                     FDNAVVNATVLRHMDEKWRQRFLLWGILIAVFGMRIVFPLAIVGVVASLGPVEVVDLA
                     LFNPDEYARLLTSAHHEIAAFGGAFLMMVFLKFFIDENKDTHWLAPIESPLTKLGRLE
                     AAQILLTLLAILATSAWIDGADKRMEFIVAGVWGLIVYIAADGLGAIMGGEEGEGGKM
                     VAKTGFAGFMYLELLDASFSFDGVIGAFALTTSLPIIAIGLGVGAMFVRSFTLMLVYR
                     GTLEAYRYLEHGAFWAIGALAAIMFVGVHMHIPETFTGLIGAVLIGAAFWSSLRHNRL
                     SVGGG"
     gene            complement(4239..4985)
                     /locus_tag="PKN25_13925"
     CDS             complement(4239..4985)
                     /locus_tag="PKN25_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV92045.1"
                     /translation="MQTDPRLEPSTPLLRLQELKIAYGGIQAVKGIALELHAGETVCL
                     IGANGAGKTTTLNAIAGVLPIAGGDIHYKGERINALPAHRRLRAGIALVPEGRGIFTR
                     LTVEENLRMGAYTRKDAAAIEADLERVYTMLPRVKERLAQVAGTLSGGEQQMVAIGRA
                     LLSRPRLLLLDEPSMGLAPLIVEKIFEVVQSVAREGVTILLVEQNANLALEFAQRGYV
                     MESGRITLTGSGSALLQDPKVRAAYLGEEA"
     gene            complement(4975..>5288)
                     /locus_tag="PKN25_13930"
     CDS             complement(4975..>5288)
                     /locus_tag="PKN25_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV92046.1"
                     /translation="YGDQRRLEIARALATEPQLLALDEPAAGMNATETGQLKQLIQQI
                     RNDGITVMLIEHDVKLVMGLCDRVAVLDFGSKIAEDVPAVVQKDEAVIAAYLGGGKHA
                     N"
BASE COUNT          730 a         1722 c         1922 g          814 t
ORIGIN      
        1 accgcggcgc tggccgacat cgtgctgccg atctgcacgc cgtgggagcg cgaggcgctg
       61 cgggtgggct tcgagggcag ccaggcggcg gaatcctggg tgcagctgcg cccggcggcg
      121 atcgcgccgc tcggcgacag ccgcgcggat gcctgggtgg tgttccggct cgcgcagcgg
      181 ctggggctgg gcgagcagtt ctggggtggc gacctcgagg cggggctggc ccatgtgctc
      241 gccccgaccg gactcacgct cgagaccctg cgcgcgcacc cgggcgggct cgcgctgccc
      301 gcggccacgc gctaccgcaa gtaccgggac gagggcttgc gcacgccgag cgggcgtatc
      361 gagctgtatt ccgagacgct gctcgagcac ggcgagtcgc cgctgccgcg cttcgccgag
      421 ccggttgatt cgcccttcgg caaggcggcg ccggaatatc cgctggtgat gaccaccgcc
      481 aagctgcgcc acttccgcca cggccaggac cgccaggcgc cctcgctgcg ccggcactct
      541 ccccaaccgg cggtgcatct gcacccggac gcggcacggg cgcgcgccat cgcggagggc
      601 gactgggtgc gggtgcgcac tgccaacggc gccatccgcc tgcgcgcgcg tttcgatgcg
      661 gcgctcgatg cgcgtgtggt gtgggtggaa tacggctggt ggcagcctgg cgcggcgggg
      721 gcggccgacg gctacgacgc gcgctcggag gcgggggcga actacagccg cctcgtctcc
      781 gaccgccgcg tcgatccggt cgccggctcg cagcccctgc gctcggggct gtgcgagatc
      841 gagccgctca gtagctcagg ttgagctggt acaccgccgt ggtgccgctg acctcgttgc
      901 cgaccagcag cagcggcttg gcgttgggag agtccttggc ggggatgaag tgcaggcctt
      961 cggggccgag gtcgccgacg ctcgccagca cggtcgaggg gtctttggtg ctccagtcct
     1021 cgcgcgggtt gaggtagtcc acgcgggtcg gcgcctgcgg gtcggtgatg tcatagacca
     1081 gcacgccgcc catgcgctcg aggccgacga aggcgaaggt cttgtcgccg atgcggccga
     1141 gcgcgacgcc ttcgggttcc gcacccttgt tgtcgctgcg attgtccagc gtgtcgcctt
     1201 cctcgtggtt ggcgttgaac caggtcgcgc aggggatgtt gcgcgcgctg ccgagcttgc
     1261 actcgtcgct ggcgaggaag cgctcgatct cggcgcccga gtcccacacc agcttgccgt
     1321 ccgcgtcgcg gatcgagaac gagcgtccgc caaaggcgta gagcgtgtcg tacatcaggc
     1381 gggtgccggc cgggttctcg ttgccggcgg tgtcgaacag gatcggcttg ccgtcggcgt
     1441 cctggcgata gcccatggtc caggtgatgt tgaggcggcc gagctggtcg tcgtcgcggc
     1501 acttgccggg ggcgccagcg accgcgccgc agtaggcgaa gaccgtcggg tcgagcaggc
     1561 cgccgtcggc gagcgcgtcg agctgcggcg gcaggtcgtc gcccttgcgg ccggaccagc
     1621 ccttggtgtt ggtgagatgc ttgacgcgga actcttcgac gaagcccttg ctggcgtcgc
     1681 cgtcccagta ggcgtcgtcg ccttcgcccc aggtacgggc gtcgccctcg ttggcggtga
     1741 ccagcagggt gcggccctgg tgggtgtacg aggcgatcgc gtcgggcagg tagaggccgc
     1801 gcacgcccgg ccaggcctgg atgacggcct tcttgtcggt gtcggaggga tcgaacgcgt
     1861 tcgcatccac accgtggtcc ttgtcgcgga gcggcaggat cgcctccacc gtggccgtgg
     1921 ccacgtcgat gcgggcgagc gcgttgttct cctgcagcgt ggcccaggcg gtggcgccgt
     1981 cggccgagac ggcgatgtac tcgggctcga ggtcctgcgc ggcgctcgcg ccggggccgt
     2041 agatgcgcac gccctgggcg cgcaacgcct tctcctggcc gttgaaggcg gtgaaggcgg
     2101 cgctgcgggc gaccggggcc gtgggggtgg agacgtcgat cacgctgatc gagccctcgg
     2161 ggtcgatcga gtagtcgtcg ttcggctcgc cttcgttggc gaccagcacg gtcttgccgt
     2221 cgggggtaaa ggtgagcatg tcgggcaggg cgccgacctc gacgctgccc agcagcgcaa
     2281 ggtcggccgc gcggtagagc gcgacccggc ccttgtcggt cttggggctg gcctggatcg
     2341 cgaccgcgac cacgccatcg cgcacggcga cgctgttgat ctcggagccg gcgagcaggg
     2401 cctgcgagtc gagccggccg acggagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnntgcc gaagagattg gtcgcgtagc cgccgatgcg ctccaggcgc agcgagatcg
     2581 gggtgcgctc gggagcgggg gcctgcgggg ccggggcggt ttcggttgca ggcggcggcg
     2641 gtggagtgtc gtcgtcgccg gtgcacgcag ccagcgtggc ggcggcgagg aggctgaggg
     2701 agaaggtctc gagggcgtgg cgcaggcggt ggctcatggt gtcggggggc ggtgggcaaa
     2761 ggcccccgaa tctatgagca cggcgtgacc ggggtgttac aggaggctgc gcgcagaccg
     2821 tccgaccggc cgccatcgtg cgcaggtgtc gccgcaatcg tttgcaggcg cagccgcaat
     2881 cgcgacttgc gtcgctccca catgcgaccc gggcgaggtg gttcgctccg gatcggggat
     2941 gcggtgcttc caccgccgcg gagccgcgat ggtcttgtgg gagcgacgcg agtcgcgatt
     3001 ctgcgcttca gcccccgcct acgctcagcc ggttgtgccg cagcgagctc cagaacgccg
     3061 cgccgatcag caccgcgccg atcaggccgg tgaaggtctc cgggatgtgc atgtgcactc
     3121 ccacgaacat gatcgccgcc agcgcaccga tggcccagaa ggcgccgtgc tccagatagc
     3181 ggtaggcctc gagcgtgccg cgatacacca gcatcagcgt gaacgagcgc acgaacatcg
     3241 cccccacgcc caggccgatg gcgatgatcg gcaggctggt ggtgagcgcg aaggcgccga
     3301 tgacgccgtc gaagctgaag gacgcgtcca gcagctccag gtacatgaag ccggcgaagc
     3361 cggtcttggc gaccatcttg ccgccttcgc cctcctcgcc gcccatgatc gcgcccaggc
     3421 cgtcggcggc gatatagacg atcagccccc acaccccggc gacgatgaac tccatgcgct
     3481 tgtccgcacc gtcgatccac gccgaggtgg cgaggatggc gagcagggtc agcaggatct
     3541 gcgcggcctc caggcggccg agcttggtca gcgggctctc gatcggcgcc agccagtggg
     3601 tgtccttgtt ctcgtcgatg aagaacttca ggaagaccat catcaggaag gcgccgccga
     3661 aggccgcgat ctcgtgatgc gccgaggtga gcaggcgggc gtattcgtcg gggttgaaga
     3721 gcgcgagatc gaccacctcg accggcccca ggctggcgac cacgccgacg atggcgagcg
     3781 ggaacacgat gcgcatgccg aacaccgcga tcaggatgcc ccacagcagg aagcgctggc
     3841 gccacttctc gtccatgtgg cgcagcacgg tggcgttgac caccgcgttg tcgaaggaca
     3901 acgaggtctc cagcaccgcc agcacggcga cgatgaaggc ggccggccag ccgccgagca
     3961 gatagccgcc gacgagcccg aagacggtga agccgatcga ccaggtgaag tacttcagat
     4021 tctgcattgg gggagggagc gaaaaaaggt gcgcaatgtt agcgctttcc ctccccgctg
     4081 cggagtgcgg tggccggctc gtgttccgcc gacttgcgct cgggcgagcg aaccgacatg
     4141 gcctccggag ccgtgcccca ggagcgacgc cagtcgcgat cggacggtcg agtcgcgccg
     4201 gcgcctccgg agaggccgga atcgcgacgc gcctcgtcct aggcctcctc gcccaggtac
     4261 gccgcgcgca ccttcggatc ctgcaacaag gcgctgccac tgccggtgag ggtgatccgc
     4321 cccgactcca tcacgtagcc gcgctgcgcg aactcgagcg cgaggttggc gttctgctcg
     4381 acgagcagga tggtgacacc ctcgcgcgcc accgactgca cgacctcgaa gatcttctcc
     4441 acgatcagcg gtgccaggcc catggacggc tcgtcgagca gcagcaggcg cgggcgcgac
     4501 agcagggcgc ggccgatcgc caccatctgc tgctcgccgc ccgacagcgt gccggcgacc
     4561 tgggccaggc gttccttgac tcgcggcagc atggtgtaga cgcgctcgag gtcggcctcg
     4621 atggcggctg cgtccttgcg cgtgtacgcg cccatgcgca ggttctcctc caccgtcagg
     4681 cgggtgaaga tgccgcgccc ttcgggaacc agcgcgatgc ctgcgcgcag acggcgatgt
     4741 gcgggcaggg cgttgatgcg ctcgcccttg tagtggatgt cgccgccggc gatcggcagt
     4801 acgccggcga tggcgttgag cgtggtcgtc ttgccggcgc cgttggcgcc gatcaggcac
     4861 acggtctcgc cggcatggag ctcgagcgcg atgcccttga ccgcctggat gccaccatag
     4921 gcgatcttca gctcctgcag gcgcagcagc ggcgtgctcg gctcgagccg gggatcagtt
     4981 tgcatgcttg cctccgccca gataggcggc gatcaccgct tcgtccttct gcaccactgc
     5041 gggcacgtcc tcggcgatct tgctgccgaa gtcgagcacc gcgacgcggt cgcacaggcc
     5101 catcaccagc ttcacgtcgt gctcgatcag catcacggtg atgccgtcgt tgcggatctg
     5161 ctggatcagt tgcttgagct ggccggtttc ggtggcgttc atgcccgccg ccggttcgtc
     5221 gagcgcgagc agctgcggct cggtggcgag cgcgcgcgcg atctccaggc ggcgctggtc
     5281 gccgtagg
//
LOCUS       DAOQBS010000168         5253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_16313_length_5253_cov_17.196806, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000168 DAOQBS010000000
VERSION     DAOQBS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16313_length_5253_cov_17.196806"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PKN25_13935"
     CDS             complement(<1..1003)
                     /locus_tag="PKN25_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNV92047.1"
                     /translation="MSRRVRPSPLLRAIERATTAMTRRALRAGADAAGKAAARGVEAM
                     SAPYRLPVSAGDWIPGQAIGPAGLRRFRLFRPADVRSSDRLPLLVMLHGCGQNAAAFA
                     RSTRMDRLAARERFLVLYPEQDLAANPQGCWNWYDTRSHRAEAEAATLIAAIDQACLL
                     YPVDRDAVAVAGLSAGASMAALLAVRWPERFCAVAMHAGVAPGAANSAAGVLAAMRGR
                     SLPPTAVERGNRPPLLVIHGSEDRVVSVRNAHAAAEQWAAALGARAADPRRVQRGARH
                     AMTVTDWRVRRRVCVRLCEVERLGHAWSGGAAGEHFSDPQGPDASTLIWRFVQGSLLG
                     "
     gene            complement(1211..1444)
                     /locus_tag="PKN25_13940"
     CDS             complement(1211..1444)
                     /locus_tag="PKN25_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92048.1"
                     /translation="MAQPTLKQRKTFALIRIFGGMVAALYLSFVVVTNMLAGHALEGE
                     LLYSALVALAGYGYAAWYLRELAAVAREERGGR"
     gene            1663..3048
                     /locus_tag="PKN25_13945"
     CDS             1663..3048
                     /locus_tag="PKN25_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNV92049.1"
                     /translation="MPSPALSLSPALLFLLVFVEGFVSLGAEILALRRLVPHVGSAIT
                     VTAPTIAFFLLALALGYQAGGQVAGDYLARVRRNFLLAAALIALGLAGPVVELAFARL
                     QPAPVAWAVVVCGVLCPVAWLLGQTVPILTNLLRHERVGARSGAALYWSTLGSFLGAL
                     TLSMGVMQWLGVGAAVLVCTGLLLAAVPALGRAAGARLPSAAAALAIAGMALVANLVL
                     PQQIETAYATYRVEAVAIPGYVAPEVLRINNSPASILDAGEPPRYARYIRRLRDLLLD
                     ELGFRGRSVLVLGAGGFTLSHREPLNRYVYVDIDPAVKEIAERDFLREPVRGEFVAAD
                     ARAHLRETAARHDAVVVDVYSALTSIPAHLVTREFWRATRAVLKPGGVLAANLILDRE
                     LASPYARSLLASIQAEYGACAVEVLFRDRPQGNVLVICSAPSTAHAAQEPALPYTDER
                     NRADLDVLRGS"
     gene            complement(3049..>5253)
                     /locus_tag="PKN25_13950"
     CDS             complement(3049..>5253)
                     /locus_tag="PKN25_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV92050.1"
                     /translation="EYLSFPDAPVPRCCMNIKQPVVDAGGRPVGVIGLAIDIEARKQQ
                     ERELVGLKNDYAAILQALPDPLFEFDIEGRCHGYRTPDEDLLAAPPEVFLGRTVREVL
                     PPEEAEVCLEALREAAAKGRSTGKVFKVEAPQGTRWFELSVAPMASAAGLTQRFNVLS
                     REVTARERMERALQEQESLLRAVVDNTPVEYWARDLDGRCTLQNAMTVAHWGDLLGQR
                     PEESAKEPAARTEWLETNRRAYAGETVHREVEYEVDGETRIFQCLVAPIRVGGEVVGI
                     FGFNQDITERKRQEHQIHELAFFDPLTRLPNRRLMFDRLERALSSSARRRHQGALLLI
                     DLDHFKELNDSHGHEAGDQLLMKVAARLGLSIREGDTAARLGGDEFVVILEDLEDVAD
                     ELLHIKAVADRIGRELGRPFQLQRGTAGETISYQCSASIGISAFGEPGVGAAELLRRA
                     DTALYQAKAAGRNAVSFFDPIMQAAVTARARLHSDLRQAIRGGQFELHYQVQVDAQRR
                     PAGAEALVRWRHPEKGLIHPGGFIDLAEETGLIVPLGHWVLATGCRQLALWARHPATA
                     HLRLAVNVSARQFRQPSFADEVRALLEQTGAPADRLKLELTESSLIEDTEAVIERMET
                     LRALGIRFALDDFGTGYSSLAYLKRLPLAQLKIDQSFVRDLMSDPNDASIVRTILALG
                     DSLGLAVIAEGVETEAQYAFLAAHGCRAYQGYLFGQPVALDVFEAGLAASDG"
BASE COUNT          690 a         1943 c         1887 g          733 t
ORIGIN      
        1 gcccaagcag gctcccctgc acgaagcgcc agatcagcgt cgatgcatcc ggcccctgcg
       61 ggtcgctgaa gtgctcgccg gcggcgccgc cgctccaggc atggccgagg cgctcgacct
      121 cgcacaggcg gacgcacacc cggcgccgca cacgccagtc ggtcaccgtc atcgcgtggc
      181 gcgccccgcg ctgcacccgg cgcggatcgg cggcgcgcgc gccgagcgcg gcggcccact
      241 gctccgcggc ggcgtgggcg ttgcgaaccg acaccacgcg gtcctcgctg ccgtggatca
      301 ccagcagcgg cggccggttc ccgcgctcga ccgcggtcgg gggcaggctg cggccgcgca
      361 tcgcggccag caccccggcc gccgagtttg ccgcgcccgg cgcgacacca gcgtgcatcg
      421 cgaccgcgca gaagcgctcc ggccagcgca ccgcgagcag ggccgccatg ctcgcccccg
      481 ccgacaagcc ggccaccgcc accgcgtcgc ggtcgaccgg gtagagcagg caggcctggt
      541 cgatcgccgc gatcagagtc gccgcctcgg cctcggcgcg atggctgcgc gtgtcgtacc
      601 agttccagca gccctgcgga ttggcggcca ggtcctgctc gggatagagc accaggaagc
      661 gctcgcgcgc ggcgagccgg tccatgcgcg tgctgcgcgc gaaggccgcg gcgttctggc
      721 cgcagccgtg cagcatcacc agcagcggca ggcgatcgct gctgcgcacg tctgccgggc
      781 ggaacagccg gaagcggcgc aggcccgccg gcccgatcgc ctgcccgggg atccagtcgc
      841 cggccgacac cggcaggcgg tagggcgcgg acatcgcctc gacgccgcgc gccgcggcct
      901 tgccggcggc gtccgcgccg gcgcggagcg cgcgccgggt catcgcggtg gtggcgcgct
      961 cgatggcacg caacaggggc gaagggcgga cgcggcggct caccacacta ccctcctgtc
     1021 gctgcgccac ctcctccccg agggaggaga gcgccagcct tcgggcggcc gtgcagacgt
     1081 gctcacgcca gacgatcgtc gcagggcgcc gcgttgcgcg tccgtgcagc ggagggcgag
     1141 ttggccgacg gcagactggc cgacggcgga ttggcggacc cagccgcggc gaggtccgtt
     1201 cgcgccgccc tcatcggccg ccgcgctcct cgcgcgccac cgcggcgagc tcgcgcaggt
     1261 accaggccgc gtagccgtag ccggcgagcg cgaccagggc ggaataaagc agctcgcctt
     1321 caagcgcatg gccggcgagc atgttggtga cgacgacgaa gctcaggtag agcgcggcga
     1381 ccatgccgcc gaagatgcgg atgagggcga aggtcttgcg ctgcttgagg gtgggctggg
     1441 ccatgggtag acggggcggg acaaggagac aggcggcagg gtagccgatt ccggccttgc
     1501 cggcatcggc cgggggagcg cagcgcaaca ctgcgcacag gcgtgctggc ctacggcggc
     1561 aacgcgccgg ataatcggtc gcctccaagg cgcccccttc accacgcgag gacctccgcg
     1621 atgcacgctc cggtctccgg cagccccggg acggcgcccg ccgtgccctc ccccgccctg
     1681 tcgctgtcgc ccgccctgct cttcctgctc gtcttcgtcg agggcttcgt ctcgctcggc
     1741 gccgagatcc tcgcgctgcg ccggctggtg ccgcacgtgg gcagcgcgat cacggtcacc
     1801 gcgccgacga tcgccttctt cctgctcgcg ctggcgctcg gctaccaggc cggcgggcag
     1861 gtggctggcg actacctcgc gcgcgtgcgg cgcaacttcc tgctcgccgc ggcgctgatc
     1921 gcgctcgggc tggcgggtcc ggtggtggag ctggccttcg cccggctgca gccggcaccg
     1981 gtggcctggg cggtggtggt gtgcggcgtg ctgtgcccgg tggcctggct gctcggccag
     2041 accgtgccca tcctcaccaa cctgctgcgc cacgagcgcg tcggcgcgcg cagcggggcg
     2101 gcgctgtact ggtcaacgct gggctccttc ctgggcgcgc tgacgctgtc gatgggggtg
     2161 atgcagtggc tgggcgtggg cgcggcggtg ctggtgtgca ccggcctgtt gctcgcagcg
     2221 gtgccggcgc tcggccgcgc ggctggtgcg cgcctgccgt cggccgcggc ggcgctcgcg
     2281 atcgcgggca tggcgctggt ggccaacctg gtgctgccgc agcagatcga gaccgcctac
     2341 gccacctacc gcgtcgaggc ggtggcgatc cccggctacg tggcacccga ggtcctgcgc
     2401 atcaacaact cgccggcctc catcctcgac gccggcgagc cgccgcgcta cgcgcgctac
     2461 atccgccgcc tgcgcgacct gctgctcgac gagctcggct tccgcgggcg cagcgtgctg
     2521 gtgctgggcg ccggcggctt cacgctctcg caccgcgagc cgctcaatcg ttacgtctat
     2581 gtcgatatcg accccgcggt gaaggagatc gccgagcgcg acttcctgcg cgagccggtg
     2641 cgcggcgagt tcgtcgccgc cgacgcgcgt gcccacctgc gcgagaccgc cgcgcgccac
     2701 gacgcggtgg tggtcgacgt ctacagcgcg ctcaccagca tccccgccca cctggtcacg
     2761 cgcgagttct ggcgcgccac ccgtgccgtg ctgaagccgg gcggcgtcct cgccgccaac
     2821 ctgatcctcg accgcgagct cgccagcccc tacgcgcgca gcctgctggc gagcatccag
     2881 gccgagtacg gcgcgtgcgc ggtcgaggtg ctgttccgcg accgcccgca gggcaacgtg
     2941 ctggtgatct gctccgcccc gtccacggcg cacgcggcgc aggaacccgc cctgccctac
     3001 accgacgagc gcaaccgcgc cgacctcgac gtgctgcgcg gcagctgatc agccgtccga
     3061 cgccgccagt ccggcctcga acacgtccag cgcgaccggc tgcccgaaca ggtagccctg
     3121 gtaggcgcgg cagccgtgcg ccgccaggaa ggcatactgc gcctcggtct cgaccccttc
     3181 ggcgatcacg gcgaggccga ggctgtcgcc gagggcgagg atggtgcgca cgatgctggc
     3241 atcgttggga tcggacatca ggtcgcgcac gaaagactgg tcgatcttca actgcgccag
     3301 cggcaggcgc ttgagatagg cgagcgagga gtaaccggtg ccgaagtcgt cgagcgcgaa
     3361 acggatgccg agcgcacgca gcgtctccat gcgctcgatc accgcctcgg tgtcctcgat
     3421 caggctgctc tcggtcagct cgagcttgag gcggtccgcc ggcgcgccgg tctgttcgag
     3481 cagcgcacgc acctcgtcgg cgaaggacgg ctgacggaac tgacgcgcgc tcacgttgac
     3541 cgcaagccgc aggtgcgcgg tcgcggggtg gcgggcccac agcgcgagct gccggcaccc
     3601 cgtcgccagc acccagtggc cgagcggcac gatcagcccg gtttcctcgg cgaggtcgat
     3661 gaatccgccc gggtggatca gccccttctc gggatgacgc cagcgcacca gcgcctcggc
     3721 gcccgcgggc cgtcgctgcg catccacctg gacctggtaa tgcagctcga actggcctcc
     3781 gcggatcgcc tggcgcaggt cggaatgcag gcgcgcgcgc gcggtgaccg cggcctgcat
     3841 gatcgggtcg aagaagctga ccgcgttgcg ccccgcggcc ttggcctggt agagcgcggt
     3901 atcggcacga cgcagcagct cggccgcgcc gacgcccggt tcgccgaagg cgctgatgcc
     3961 gatgctggcc gagcactggt aggagatcgt ctcgcccgcc gtgccgcgct gcagctggaa
     4021 gggtcggccg agctcccggc cgatgcggtc cgcgaccgcc ttgatgtgca ggagctcgtc
     4081 ggcgacgtcc tcgaggtcct ccaggatcac cacgaactcg tcgcccccca ggcgcgcggc
     4141 cgtgtcgccc tcgcggatgc tcagcccgag gcgcgcggcg accttcatca gcagctggtc
     4201 tccggcctcg tggccgtggg aatcgttgag ctccttgaag tggtcgaggt cgatcagcag
     4261 cagcgcgccc tggtgccggc gacgcgcact gctgctcaag gcccgctcca ggcggtcgaa
     4321 catcagacgc cggttgggca gccgggtcag cggatcgaag aaggcgagct cgtggatctg
     4381 gtgctcctgg cgcttgcgct cggtgatgtc ctggttgaaa ccgaagatgc ccaccacctc
     4441 gccgcccacg cggatcggcg cgaccaggca ctggaagatg cgcgtctcgc cgtcgacctc
     4501 gtactcgacc tcgcgatgga cggtctcgcc ggcgtaggca cggcggttgg tctccagcca
     4561 ctcggtccgt gccgcgggtt ccttcgcgct ctcttccgga cgctggccga gcaggtcgcc
     4621 ccagtgcgcc accgtcatcg cgttctgcag ggtgcagcgc ccgtcgaggt cgcgcgccca
     4681 gtattccacc ggggtgttgt ccaccacggc gcgcagcagc gactcctgct cctgcagcgc
     4741 gcgctccatg cgctcgcggg cggtcacctc gcgcgaaagc acgttgaagc gctgcgtcag
     4801 gcccgccgcg ctggccatcg gcgccaccga gagctcgaac cagcgcgtgc cctggggcgc
     4861 ctccaccttg aacaccttgc ccgtggagcg ccccttcgcc gccgcctcgc gcagggcctc
     4921 gagacagacc tcggcctctt ccggcggcag cacctcgcgc acggtgcgcc ccaggaacac
     4981 ttcgggcggc gcggcgagca gatcctcgtc gggagtgcgg tagccgtggc agcggccttc
     5041 gatgtcgaac tcgaacaagg gatcgggcag ggcctggagg atcgcggcat agtcgttctt
     5101 gaggccgacc agctcccgct cctgctgctt gcgcgcctcg atgtcgatgg caaggccgat
     5161 cacgccgacc gggcggccgc cggcgtcgac gaccggctgc ttgatgttca tgcagcagcg
     5221 cggcacgggc gcgtcgggaa agctcagata ttc
//
LOCUS       DAOQBS010000169         5118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_16977_length_5118_cov_16.312068, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000169 DAOQBS010000000
VERSION     DAOQBS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16977_length_5118_cov_16.312068"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKN25_13955"
     CDS             <1..741
                     /locus_tag="PKN25_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNV92051.1"
                     /translation="AEHERVWLNLAGYCLRPGLGAALDDWRVDQLWALFGQGLQYVRE
                     GNNWSEWWTLWRRCAGGLDEARQLQVLEVLAEHLEDTDAARRRGDPVQGSYDDMVRLA
                     AALERVPAMHRIEVGKWLLERLARPAEKAHTWWALGRVGARESLYGSAHNVVPPETAA
                     EWLQAVLALDWKVVEPAAFAAAQLARLTGDRARDLAPELRDEVARRLAALRAPQSWIT
                     MVREVVSLDDADRRRSFGEALPPGLRLL"
     gene            complement(805..1119)
                     /locus_tag="PKN25_13960"
     CDS             complement(805..1119)
                     /locus_tag="PKN25_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HNV92052.1"
                     /translation="MPILLQVDFPYAGPWGEDMAQAMRGLAESIAQEPGLLWKIWTEN
                     RDSGEAGGIYLFADRADAERYLEMHRARLLGFGVPEVHAKLFEVNAPLSAIDRAPLAG
                     LQ"
     gene            1448..2326
                     /locus_tag="PKN25_13965"
     CDS             1448..2326
                     /locus_tag="PKN25_13965"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNV92053.1"
                     /translation="MSTDSAALHYDTIARAIAFVRARPRHTPPSGAVAEALGLDLGQW
                     QQVLAEWAGITPERFLHCLGRAYARERLSPTPERLPERDAGDPAPSDLARAVPLTWEP
                     MPPASGRTVALGHGLAATPFGDALLAWTTRGVCHLAFRCRDEAALLVELRSLWSAAAP
                     VRDDAHAHRLAQRIFPSRAPSTPVAAATGASGPAEAANPAPGGVHLILRGTPFQVRVW
                     SALMRIPPGRLMSYRQLATLLGMPAAPRSVGSAIAANTLGYLVPCHRLIRADGDAGEF
                     RWGRERKLAMLAWEAT"
     gene            2323..2844
                     /locus_tag="PKN25_13970"
     CDS             2323..2844
                     /locus_tag="PKN25_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HNV92054.1"
                     /translation="MNTRRSPDYLAGYPPALVHQVRTLIEQGGLADALRRRYPQGHAV
                     RSDSALHDYVQALKAAHLRSAPPLAKVRFDNTLHVIRNALGTHTAISRVQGSRLQAKR
                     EIRIATLFRAAPEAFLRMIVVHELAHLRVREHDKAFYQLCLHMEPDYHQYEFDLRTYL
                     SYRDAGGEALWGA"
     assembly_gap    2939..3038
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3184..>5118)
                     /gene="katG"
                     /locus_tag="PKN25_13975"
     CDS             complement(3184..>5118)
                     /gene="katG"
                     /locus_tag="PKN25_13975"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNV92055.1"
                     /translation="WWPADWGHYGGLMIRMAWHAAGTYRIADGRGGGGTGNQRFAPLN
                     SWPDNGNLDKARRLLWPIKHKYGNKVSWADLIILAGNMAYESMGFKTFGFAFGREDIW
                     HPEKDIYWGSEKEWLAPSGSEGSRYSGQRDLENPLAAVMMGLIYVNPEGVDGKPDPIK
                     TAHDMRVTFARMAMNDEETVALTAGGHTVGKAHGNGSAAVLGPAPEGADLEEQGFGWM
                     NHTTRGIGRDTVTSGIEGAWTSHPTKWDNGYFDMLFKHDWWLQKSPAGAWQWEPVNIA
                     EEDMPVDVEDPSIRRKPMMTDADMALRFDPAYRKIAERFRNDQAYFEDVFARAWFKLT
                     HRDMGPKARYLGPDVPAEDLIWQDPVPAGNKTYDVAAVKARIAAAGLSVGDMVATAWD
                     SARTFRGSDYRGGANGARIRLAPQKDWEGNEPARLARVLAAYEKIAAETGASIADVIV
                     LGGNVGLEQAIKAAGFNVEVPFAPGRGDASQAQTDVESFEVLEPVHDGFRNWQKKDYV
                     VQPEEMLLDRAQLLGLTAPEMTVLVGGLRVLGTNHGGSGHGVFTDRVGALTNDFFVNL
                     TDMSYSWKPTGRNSYDIVERKSGAKKWTATRVDLVFGSNSILRAYAEVYAQDDNQEKF
                     VRDFVAAWTKVMNADRFDLR"
BASE COUNT          688 a         1837 c         1741 g          752 t
ORIGIN      
        1 gccgagcacg agcgcgtgtg gctcaacctc gccggctact gcctgcgccc cggcctcggc
       61 gcggcgctcg acgactggcg cgtcgaccag ctgtgggcgc tgttcggcca gggcctgcag
      121 tacgtgcgcg agggcaacaa ctggagcgag tggtggacgc tgtggcggcg ctgcgccggc
      181 ggcctcgacg aggcacgcca gctgcaggtc ctggaggtgc tggccgagca cctcgaagac
      241 accgacgccg cccgccgccg cggcgacccg gtgcagggca gctacgacga catggtgcgc
      301 ctggccgctg ccctcgaacg cgtgccggcg atgcaccgca tcgaggtcgg caagtggctg
      361 ctcgagcgcc tcgcccgccc cgccgaaaag gcccacacct ggtgggcgct cggccgcgtc
      421 ggcgcgcgcg agtcgctcta cggcagcgca cacaacgtgg tgccgccgga gaccgccgcc
      481 gagtggctgc aggccgtgct cgcgctcgac tggaaggtcg tcgagcccgc cgccttcgcc
      541 gccgcccagc tcgcccgcct caccggcgac cgcgcgcgcg acctcgcgcc cgaactgcgc
      601 gacgaggtcg cgcgccgcct cgccgccctc cgcgccccgc agagctggat caccatggtg
      661 cgcgaggtcg tcagcctcga cgacgccgac cgccgccgca gcttcggcga agcgctgccg
      721 ccgggcttgc ggctgctgta gcgctgcgcg gtggagggag gagggcgcgc cgtcgccggc
      781 tcgatgccga cggcgcccat ccggtcactg caggccggcg agcggcgcgc ggtcgatcgc
      841 cgacagcggc gcgttgacct cgaacagctt cgcatgcacc tcgggcacgc cgaagccgag
      901 caggcgcgcg cggtgcatct ccaggtagcg ctcggcgtcg gcgcggtcgg cgaacaggta
      961 gatcccgccc gcctcgccgc tgtcgcggtt ctcggtccag atcttccaca gcaggccggg
     1021 ctcctgcgcg atggactccg ccaggccccg catggcctgc gccatgtcct cgccccaggg
     1081 gccggcatag ggaaaatcga cttgaagaag gatgggcacg cgcgatctcc tgtgcgtcgg
     1141 ggtcggaacg gaaggcaagc cgggtcgcgc cgggagggtg gaccgcggcg ttcgcacggt
     1201 tcgggacggc tccggactca tcggccattc taccgtcgcg gcgcaggtat ccggtcgcgg
     1261 cgccaaacga accgacgcat cgcgacttgc gtctcctatg gaaaaccggg caggtttccg
     1321 gagcgcccgc aggagcgacg ccagtcgcga tagcagcgcg tcctgcacaa gcctcgccgg
     1381 ccaggccggc taccattgtc gaccgcaagg ccaccccgcc ttgtcaccca tcacacccgc
     1441 acccgccatg tccaccgact ccgctgccct gcactacgac accatcgccc gcgcgatcgc
     1501 cttcgtgcgc gcccgccccc gccacacgcc accgtccggg gcggtcgcgg aggcgcttgg
     1561 cctcgatctc gggcagtggc agcaggtgct cgcggaatgg gcggggatca cgcccgagcg
     1621 cttcctgcac tgcctggggc gggcgtatgc acgcgagcgc ctgtcgccga cgccggagcg
     1681 gctcccggag cgcgacgccg gcgatcccgc accgtcggac ctggcccgcg ccgtgccgct
     1741 cacctgggag ccgatgcccc ccgcctcagg gcgcaccgtc gcactcggcc acggccttgc
     1801 agccacgccc ttcggcgacg cgctgctggc gtggaccacc cgcggcgtgt gccatctcgc
     1861 ctttcgctgt cgtgacgagg cggccctgct cgtcgagcta cgcagcctct ggtccgccgc
     1921 cgcgccggtg cgcgacgatg cgcacgcaca ccgcctggcg cagcggatct tcccgtcgcg
     1981 cgcgccttcc accccggtgg ctgcagccac cggggcttcc gggcccgccg aagcggcgaa
     2041 ccccgcccca ggcggcgttc acctgatcct gcgcggcacg cccttccagg tccgcgtctg
     2101 gtccgccctc atgcgcatcc cgcccggtcg cctgatgtcc taccgccagc tcgccaccct
     2161 gctcggcatg ccggctgcac cgcgcagcgt cggcagcgcg atcgcggcca acacgctcgg
     2221 ctacctcgtc ccctgccatc gcctgatccg cgcggacggc gacgcgggcg agttccgctg
     2281 gggccgcgag cgcaagctgg cgatgctggc ctgggaggcc acttgaacac gcggcgcagc
     2341 cccgactacc tggccggcta cccaccggcg ctggtgcatc aggtgagaac gctgatcgag
     2401 cagggcggac tggccgacgc gctgcgccga cgctacccgc aggggcacgc ggtgcgttcg
     2461 gacagcgcgc tccacgacta cgtgcaggcg ctcaaggccg cccacctgcg cagcgcgccg
     2521 ccactcgcca aggtgcgctt cgacaacacc ctgcatgtga tccgcaacgc gctcggcacc
     2581 cacaccgcga tctcgcgcgt gcagggttcg cgcctccagg ccaagcgcga gatccgcatc
     2641 gccacgctgt tccgcgccgc gccggaggcc ttcctgcgca tgatcgtggt gcacgaactc
     2701 gcccacctgc gcgtgcgcga gcacgacaag gccttctacc agctctgcct gcacatggag
     2761 ccggattatc accagtacga gttcgacctg cgcacctacc tgagctaccg cgacgcgggc
     2821 ggcgaggcgc tgtggggggc gtagggatgc cggagtccag atacgcagcg cgcgtcgcgg
     2881 ccgcaccgca cgatcgcgac ttgcgtcgct cccaccgggc acggcggaag cacggcggnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc cgtgggagcg acgcgagtcg
     3061 cgatcggccg gcacggctcg acacgacgtg gcgaggggca catcccggcc gtccccgaac
     3121 gaaaccgccc acaccgcgcg gctgcacggt gtgggcggga gggtgctgca ggcgggaacc
     3181 gcgtcagcgc aggtcgaaac gatccgcgtt catgaccttg gtccacgcgg cgacgaagtc
     3241 gcgcacgaac ttctcctgat tgtcatcctg ggcatacacc tcggcgtagg cgcgcaggat
     3301 ggaattggag ccgaacacca gatcgacgcg ggtcgcggtc cacttcttcg cgccgctctt
     3361 gcgctcgacg atgtcgtagc tgttgcgccc ggtgggcttc cagctgtagg acatgtcggt
     3421 cagattgacg aagaagtcgt tggtcagcgc gccgacgcgg tcggtgaaca cgccgtgccc
     3481 gctgccgccg tggttggtgc cgagcacgcg caggccgccg accagcacgg tcatctcggg
     3541 cgcggtcagg ccgagcagtt gggcgcggtc gagcagcatc tcctcgggct ggaccacata
     3601 gtccttcttc tgccagttgc ggaagccgtc atgcacaggc tccagcacct cgaaggactc
     3661 gacgtccgtc tgcgcctggc tggcgtcgcc tcgacccggg gcgaagggca cctcgacatt
     3721 gaagccggcg gccttgatcg cctgctccag gccgacgttg ccaccgagca cgatcacgtc
     3781 cgcgatgctg gcgccggtct cggcggcgat cttctcgtag gccgccagca ccctggccag
     3841 ccgcgccggt tcgttgcctt cccagtcctt ttgcggggcg aggcggatgc gggcgccatt
     3901 ggcgccgccg cggtaatccg agccgcggaa ggtgcgtgcg ctgtcccagg cggtggcgac
     3961 catgtcgccg accgacaggc cggcggcggc gatcctcgcc ttcaccgcgg cgacgtcgta
     4021 ggtcttgttg ccggcgggca ccgggtcctg ccagatcagg tcttcggcag gcacgtcggg
     4081 accgaggtag cgcgccttcg ggcccatgtc gcggtgggtg agcttgaacc aggcgcgcgc
     4141 gaagacgtcc tcgaagtagg cctggtcgtt gcggaagcgc tctgcgatct tgcggtacgc
     4201 cgggtcgaat cgcagcgcca tgtcggcgtc ggtcatcatc ggcttgcggc ggatcgacgg
     4261 gtcttccacg tccaccggca tgtcttcctc ggcgatgttc accggctccc actgccaggc
     4321 gccggcgggc gacttctgca gccaccagtc gtgcttgaac agcatgtcga agtagccgtt
     4381 gtcccacttg gtcggatgcg aggtccacgc gccctcgatg ccgctggtca cggtgtcgcg
     4441 accgatgccg cgggtggtgt ggttcatcca gccgaagccc tgctcctcga gatccgcacc
     4501 ttcgggcgcg gggccgagga cggcggcgct accgttgccg tgcgccttgc cgacggtgtg
     4561 gccgccggcg gtgagcgcca cggtttcctc gtcgttcatc gccatgcggg cgaaggtcac
     4621 gcgcatgtcg tgggcggtct tgatcgggtc gggcttgccg tcgacgccct ccgggttcac
     4681 atagatcagg cccatcatca ccgcggcgag cggattctcg aggtcgcgtt ggccggagta
     4741 gcgcgagccc tcgctgccgc tgggggcgag ccattccttc tcggagcccc agtagatgtc
     4801 cttctcggga tgccagatgt cctcgcggcc gaaggcgaag ccgaaggtct tgaagcccat
     4861 cgactcgtag gccatgttgc cggcgaggat gatgaggtcg gcccagctca ccttgttgcc
     4921 gtacttgtgc ttgatcggcc acagcaggcg gcgcgccttg tcgaggttgc cgttgtcggg
     4981 ccaggagttc agcggcgcga agcgctggtt gccggtgccg ccgccgccac gaccgtcggc
     5041 gatgcggtag gtgccggcgg cgtgccaggc catgcggatc atcaggccgc cgtagtggcc
     5101 ccagtcggcc ggccacca
//
LOCUS       DAOQBS010000170         5104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_17053_length_5104_cov_14.933848, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000170 DAOQBS010000000
VERSION     DAOQBS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17053_length_5104_cov_14.933848"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..612)
                     /locus_tag="PKN25_13980"
     CDS             complement(34..612)
                     /locus_tag="PKN25_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNV92056.1"
                     /translation="MNRIARLTAALLVSAAAASPALAAPQTYVVDGTHTFPSFSYSHF
                     GLSTQLSKFDKTTGTVTLDKEAKTGAVDITIDMKSVNTGYETFDGHIQGEDFLDTAKY
                     PTATFKSTKVSFDGDKPATIDGELTIKGVTKPVTLKVSHFTTMPHPMLKKDTIGANAS
                     TVIKRSEFNAGKYAPHVGDDVTITVSLEAIQN"
     gene            complement(698..1258)
                     /locus_tag="PKN25_13985"
     CDS             complement(698..1258)
                     /locus_tag="PKN25_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNV92057.1"
                     /translation="MKRIALALLASFAFAATTAHAVEYGEVQPDKSRIAFDYQQMGVA
                     MQGTFGKFASALRFDPAAPQTASARIEVELASVDTGSEEGDDEVARKTWFDTKGFPVA
                     RFESSTVKPLGGNQYEVAGKLTIKGTTKDVVVPATFTPQGGSGVFEGALTIRRGDFSI
                     GEGAWKAFDVVANDVVIRFRITAAAR"
     gene            complement(1294..1830)
                     /locus_tag="PKN25_13990"
     CDS             complement(1294..1830)
                     /locus_tag="PKN25_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNV92058.1"
                     /translation="MDTHYTATAKLLHWLIALMIFGMLGFGFYMTGLDLSPTKLELYS
                     WHKWAGVTVFLLVLVRLAWRIGHRPPALPAHMPAIERLAAHAGHHLLYLLMLAIPLSG
                     WLMSSAKGFQTVWFGVLPLPDLLAKDKALGNLLETVHVALNFTLIAVLLGHVGAALKH
                     HFIDRDDVLTRMLPRRRG"
     gene            complement(2060..>5104)
                     /locus_tag="PKN25_13995"
     CDS             complement(2060..>5104)
                     /locus_tag="PKN25_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNV92059.1"
                     /translation="SASADATSPSARLPAYAAGRVEARRGRGDPLPADARAPLEDHFG
                     RDFADVRLHSDAEAARLTAALGARAFTSGRDIYLAPGTVASDTEEGRHLLAHELAHVV
                     QQDGQAAATLARAIEPPPPARYHETERSAAALAALDRLEIPAVKARHLPLYSGLAQAH
                     QLKRVRGYGREGADQRSVWSRQVEVGADAVRERLTERGIPVPADPSGRVRLKLHRGAR
                     TTSKRLSELQTLLRIPEWDREGRRRDFQVDHIVELQVSGQMGSGVGNSVENMELLDQP
                     SNSSSGGTIRSSIYRKLDGFLDTLEPRPDRASFLRGHDLVFTSVAATGAGSAATGSAW
                     WTRAEIEQAHTLGTASAPTAAEDGDRAGSAEEFILSAAPGGIAVGRFRHSPGAAPTIG
                     ATQARALAGLRITAIALTDLTGTPDGPVGTLSAEWDLPADWQPANPAITISLQGDGEY
                     RGYPSALPGLDLEYRHLSPVSFTRISTEDGELYAEGTLTPSIPILAAPLTVQLRGREL
                     GFALDYGPEQVSLPIPRTTIDDAWVSVFYSTTRGLGVGGDILYSIEGAGSGELSASVS
                     TGGGVAFEGGFTFDPALFDRARVRAWWRDGRLGAEGTIGIDTPDKIRGIRSATASVRV
                     DEGRWSFNGSAELSVPGLSQASIAIRQGEGGLELAGDVALATNPAIRSGTLHVECAQT
                     DGEWKVAASGTAQPAIPGVDAELAVTYADGAFDARFSGAFRRGMLSGQLSVGATNRAV
                     AADGSPGGPPSAPDAPIVVYGSGSATVRIAPWLQGSAGLRVAPDGELTVSGEIALPAS
                     LEIFSRLEYDKRLFGMSTQIPIVPGVVAEVGGNLSANASVGPGALDRLAVRIEYNPAH
                     EDDTHVTGEAHLEVPAQAGLRLGARAGVGLGITGASATGGLEIGGALGIAGAAEAGVR
                     IDWMPSRGLEIDAEAALHAQPRFRFDVSGYVAVTVLGASLYDERIELAAYELGSGLEF
                     GVRFPVTYREGEPFDLSLDDLEFQVPEVDPAAMISQLGETIF"
BASE COUNT          718 a         1590 c         1979 g          817 t
ORIGIN      
        1 cgccgaattc gcgcctgccg ccgcgcctgc agcttagttc tggatggctt ccagcgagac
       61 ggtgatggtc acgtcgtcgc ccacgtgcgg ggcatacttg ccggcgttga actcgctgcg
      121 cttgatcacg gtgctggcgt tggcgccgat ggtgtccttc ttgagcatcg ggtgcggcat
      181 ggtggtgaag tggctcacct tcagcgtcac cggcttggtc acgcccttga tggtcagctc
      241 gccgtcgatg gtggccggct tgtcaccgtc gaagctcacc ttggtcgact tgaaggtggc
      301 ggtcgggtac ttggcggtgt cgaggaagtc ctcgccctgg atgtggccgt cgaaggtttc
      361 gtagccggtg tttaccgact tcatgtcgat ggtgatgtcg accgcgccgg tcttcgcttc
      421 cttgtccagc gtcacggtgc cggtcgtctt gtcgaacttg gagagctggg tcgacaggcc
      481 gaagtggctg tacgagaagc tcgggaaggt gtgggtgcca tcgacgacgt aggtctgggg
      541 cgcggccagc gcgggcgagg cggcagcggc ggagacgagg agggcggcgg tgaggcgggc
      601 gatacggttc atgtggtggt cctttgtgga gggcagatgg gtggaacgga agggttggaa
      661 cgatggcgcg ggaacgatgc tgcccggctg ccgcggctca gcgtgccgcg gcggtgatgc
      721 ggaagcggat cacgacgtcg ttggcgacca cgtcgaaggc cttccaggcg ccttcgccga
      781 tcgagaagtc gccgcgccgg atggtcaggg cgccctcgaa gacgccgctg ccgccctgcg
      841 gggtgaaggt ggcggggacc acgacgtcct tcgtcgtgcc cttgatggtg agcttgccgg
      901 cgacctcgta ctggttgccg ccgagcggtt tcaccgtgct cgactcgaag cgcgcgaccg
      961 ggaagccctt ggtgtcgaac caggtcttgc gggcgacctc gtcgtcgcct tcctcgctgc
     1021 cggtgtcgac gctggcgagc tcgacctcga tgcgggccga ggcggtttgt ggcgcggcgg
     1081 gatcgaagcg cagcgcgctg gcgaacttgc cgaaggtgcc ctgcatcgcc acccccatct
     1141 gctggtagtc gaaggcgatg cggctcttgt cgggctggac ctcgccgtat tccaccgcgt
     1201 gggcggtggt cgcggcgaag gcgaaggagg cgagcagggc gagggcgatg cgtttcatgg
     1261 cgcgggttcc tgtgtgcgat ggaggaggcc ggttcagccc cggcggcgcg gcagcatgcg
     1321 ggtcagcacg tcgtcgcggt cgatgaagtg atgcttgagc gcggcgccga cgtggccgag
     1381 cagtacggcg atcagcgtga agttgagcgc cacgtgcacg gtctcgagca gattgccgag
     1441 cgccttgtcc ttggcgagca ggtcgggcag cggcagcacg ccgaaccaca ccgtctggaa
     1501 gcccttggcc gagctcatca gccagccgct gagcgggatc gcgagcatca gcaggtacag
     1561 caggtggtgg cccgcatgcg cggccaggcg ctcgatggcc ggcatgtgcg cgggcagcgc
     1621 cggcgggcgg tggccgatcc gccaggccag gcgcaccagc acgagcagga acaccgtcac
     1681 gcccgcccac ttgtgccagg agtagagctc cagcttggtg ggcgacaggt ccagacccgt
     1741 catgtagaag ccgaatccga gcatgccgaa gatcatcagc gcgatgagcc agtgcagcag
     1801 cttggcggtg gcggtgtagt gcgtgtccat gtgcttggtc cttcggccgt cgatcggggg
     1861 cgccggccag atgttcgtgt tggttgcact ttagactcat ccacaaggct taaacagccg
     1921 aagtcgggca aaggttagtt gctgatttgg caacaattaa gcctgttttc cgctgaggcc
     1981 gggtctcggg ttgcgcgatt cgctccctcc gagcggggga tggggtcggg tcgctttcac
     2041 ctcccgccct ctccgcgaat cagaagatcg tctcccccag ctggctgatc atcgccgccg
     2101 gatccacctc ggggacctgg aactcgaggt cgtcgaggga gaggtcgaag ggttctccct
     2161 cacggtaggt gacggggaag cggacgccga actcgaggcc agagccgagc tcataggcgg
     2221 cgagctcgat gcgctcgtcg tagaggctgg cgccgagcac ggtgacggcg acgtagccgg
     2281 aaacgtcgaa gcggaagcgc ggttgggcgt ggagcgcggc ctcggcgtcg atctcgaggc
     2341 cgcgcgaggg catccagtcg atgcgcacgc cagcctcggc agcccctgcg atgccgaggg
     2401 cgccgccgat ctcgaggccc ccggtggcac ttgcgccggt gatgccgagg ccgacgccgg
     2461 cacgcgcgcc gagccgcagg cctgcctgcg ccggaacctc gaggtgggcc tcgccggtga
     2521 cgtgggtgtc atcctcgtgt gcggggttgt attcgatgcg gacagcgagc cggtccagtg
     2581 ccccggggcc gacgctggcg ttggcactga gattgccgcc gacctcggcg accacgccgg
     2641 ggacgatggg gatctgcgtc gacatgccga acaggcgctt gtcgtactcc agccgggaga
     2701 agatctccag cgaagcgggc agggcgatct cgccggacac ggtgagctcg ccgtcgggag
     2761 cgacccgcag gcccgcgctg ccctgcagcc agggggcgat ccgcacggtc gcggagccgc
     2821 tgccgtagac cacgatgggc gcatccggag cgctgggcgg gccgccgggg ctcccgtccg
     2881 cagcgacggc gcggttggtg gcgccgacgc tgagctggcc ggagagcatg ccgcggcgga
     2941 aggcgccgga gaagcgcgcg tcgaaggcgc cgtccgcata ggtgacggcg agctccgcgt
     3001 cgacgccggg gatcgcaggc tgagcggtgc cgctcgccgc gaccttccac tcgccgtcgg
     3061 tctgcgcgca ttcgacgtgc agggtgccgg agcggatggc ggggttggtg gcgagggcga
     3121 cgtcgcccgc gagctcgagt ccgccctcgc cctggcggat cgcgatcgag gcctggctga
     3181 gaccgggcac ggagagttcg gcactgccgt tgaacgacca gcgcccttca tcgacacgga
     3241 ccgaggcggt cgcgctgcgg atgccacgga tcttgtccgg ggtatcgatg ccgatcgtgc
     3301 cttcggcgcc caggcgacca tcacgccacc aggcgcggac gcgagcgcgg tcgaagaggg
     3361 cgggatcgaa agtgaagccg ccctcgaagg cgacgccccc gccggtcgag accgaggcgc
     3421 tgagctcgcc gctgcctgcc ccctcgatcg aatacaggat gtcgcctcct acgcccagtc
     3481 cgcgggtggt cgaatagaag acgctgaccc aggcgtcgtc gatcgtcgtg cgcgggatcg
     3541 gcaggctgac ctgttccgga ccgtagtcga gcgcgaagcc gagctcgcgt ccgcgcagtt
     3601 gcaccgtcag cggagctgcg aggatgggga tcgagggcgt gagcgtgccc tcggcgtaca
     3661 gctcgccgtc ttcggtggag atgcgggtga aactcaccgg gctgagatgg cggtactcga
     3721 gatcgaggcc gggaagcgcc gacgggtagc cgcggtactc gccatcgccc tggagcgaga
     3781 tcgtgatcgc aggattggcg ggttgccagt cggcgggcag gtcccattct gccgagagcg
     3841 tgccgacggg gccgtcgggc gtgccggtaa ggtcggtgag cgcgatcgcc gtgatgcgca
     3901 ggccggcgag cgcgcgcgcc tgggtggcgc cgatcgtggg cgcggcaccg gggctgtggc
     3961 ggaagcggcc cacggcgatg ccgccggggg cggcgctgag gatgaactcc tcggcgctgc
     4021 cggcgcggtc gccgtcttcg gcggcggtgg gggcgctcgc ggtgccgagg gtgtgggcct
     4081 gctcgatctc ggccctggtc caccacgccg agcccgtcgc cgccgagccc gcgccggttg
     4141 ctgcgacgct ggtgaagacg aggtcgtgtc cgcgcaggaa gcttgctcgg tcgggtcgag
     4201 gctccagggt atcgaggaag ccgtcgagct tgcgatagat gctgctgcgg atcgtgccgc
     4261 ccgagctcga gttcgagggc tggtcgagga gctccatgtt ctcgacgctg ttgcccaccc
     4321 ccgagcccat ctggccggag acctgcagtt cgacgatgtg gtcgacctgg aaatcgcgcc
     4381 tgcggccctc gcggtcccat tcggggatgc gcagcagggt ctgcagctcg gacaggcgtt
     4441 tcgacgtcgt acgtgcgccg cgatgcagtt tcaggcgtac gcggccgctg gggtcggcgg
     4501 gaaccgggat gccgcgttcg gtcaggcgtt cgcgcacggc gtcggcgccg acctcgacct
     4561 ggcggctcca taccgagcgc tggtcggcgc cctcgcgtcc gtagccacgc acgcgcttga
     4621 gctggtgcgc ctgcgcgagg ccgctgtaga gcggcaggtg gcgcgccttc accgcgggga
     4681 tctcgaggcg atccagtgcg gcgagggcgg cagcgctgcg ctcggtctcg tggtagcggg
     4741 ccggaggggg cggttcgatc gcgcgcgcga gtgtcgccgc ggcctggcca tcctgctgca
     4801 cgacgtgggc gagttcgtgc gcgaggaggt gacgcccctc ctcggtgtcg gaggcgacgg
     4861 tgcccggtgc gaggtagatg tcgcgccccg acgtgaacgc gcgtgcgccg agcgcagcgg
     4921 tgaggcgcgc cgcttcggcg tccgaatgga ggcgcacgtc ggcgaagtcg cgaccgaagt
     4981 ggtcctcgag cggggcgcgg gcatcggccg gcagcggatc gccgcgcccg cggcgcgctt
     5041 cgacacggcc ggcggcatag gcgggaaggc gcgcggaggg cgaggtcgcg tcggcggagg
     5101 cgga
//
LOCUS       DAOQBS010000171         5083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_17163_length_5083_cov_14.279236, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000171 DAOQBS010000000
VERSION     DAOQBS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17163_length_5083_cov_14.279236"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PKN25_14000"
     CDS             <1..806
                     /locus_tag="PKN25_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNV92060.1"
                     /translation="EKNAPLLEPWQREVVRIVRKIAQYFFPQRQTQVMNEGWATFWHY
                     TLLNHLYDEGLLADSFMLEFLQSHTNVVAQPPYNSRWFGGINPYALGFAMWRDIRRIC
                     EDPDEEDRRWFPEIAGSDWLETFDFAMRNFKDESFVAQYLSPKVMRDFRLFAVLDDDH
                     EDKLEVSAIHDDGGFRKLREILSDQYNLGAREPNVQVWNVDLRGDRSLTLRHTPFRRR
                     PLGDGTDEVMRHLARLWGFTVRLERADDDGKVELLHEIRGETTRPRPPG"
     gene            945..2486
                     /locus_tag="PKN25_14005"
     CDS             945..2486
                     /locus_tag="PKN25_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HNV92061.1"
                     /translation="MFSPLRPIYPVAGIREIEDKLIPNARPPLMERAGRAAAQDAVRL
                     IMDRPGPILIACGPGNNGGDGFVMARQLAQAGREVVVAFCSAAERLPAEAAKAHADYL
                     AAGGSIVSDLPAAPANGWALVVDALFGIGLGRPIEDRYASWIHTLNAQPCPRMALDVP
                     SGLDADTGSPLGATFRATHTTTFIALKPGLLTNDGPDHCGEISVQRIEIDAPAWLPAR
                     GHAIASSLFRHLLQPRPRNTHKGLYGDAAILGGNAGMVGAALLAGRAALWLGTGRVYV
                     GLLDPAGPAVDFAHPELMLRRAETLPERLSALAIGPGLGTQGASANVLADALARPIPL
                     LLDADALNLLSADAALRRVLCTRGAATVLTPHPAEAARLLGSDTASVQADRLRAALEL
                     ARRYRALVVLKGCGSIVATPDERWFINGSGHSGMASAGMGDVLSGLVTGLLAQGWPPE
                     SALIAGVHLHGAAADRLAREGIGPVGLSASETIDAARGVFNGWLIEAQREPRNTGPHS
                     PRTGR"
     gene            2619..3464
                     /gene="nadC"
                     /locus_tag="PKN25_14010"
     CDS             2619..3464
                     /gene="nadC"
                     /locus_tag="PKN25_14010"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNV92062.1"
                     /translation="MLSEQLRVEIQRNIAASLAEDIGTGDLTARLIAPDTEARGRVIT
                     REDAVICGTAWFDAAFAALSPAAVVHWHVKDGDSVKAGQTLCEIAARARVLLTAERTA
                     LNFLQLLSGTATVTRRFVDAVAGTRAKIVDTRKTLPGLRLAQKYAVAIGGGTNHRVGL
                     YDGILVKENHIIAAGGIREVVEQASAIAPSNVFIEVEVETLGQLREALDAGVKMILLD
                     NMTLEQMCEAVAINAGRAELEASGGVNLERVRAIAETGVDRISIGTLTKDVRALDLSL
                     RHVER"
     gene            3481..4074
                     /locus_tag="PKN25_14015"
     CDS             3481..4074
                     /locus_tag="PKN25_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HNV92063.1"
                     /translation="MAASAPLPPAAPAASDPPALPAWPMVPACYGWLALDARGGWRLR
                     GEIITHPGLLAFLDAHYTGDAHGNWGVDNGPQRVYVELAAAPLILRLHPDGRLVAHTG
                     REATTTGPVLVDADGCAFLATDLGPAMIDDRDLALFAAELRQLEGTPAGDEDLLALID
                     GQSGPALRWREVDVAFCARAAIPGRLGFQPHPQAIGT"
     gene            4228..4312
                     /locus_tag="PKN25_14020"
     tRNA            4228..4312
                     /locus_tag="PKN25_14020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4262..4264,aa:Leu,seq:gag)
     gene            complement(4435..>5083)
                     /locus_tag="PKN25_14025"
     CDS             complement(4435..>5083)
                     /locus_tag="PKN25_14025"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304059.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNV92064.1"
                     /translation="GNFGNICAGHIARQMGLPVAKLILATNENDVLDEFFRSGVYRPR
                     KAAETHVTSSPSMDISKASNFERFVFDLVGRDPSKVAALWAEVDAGRAFDLSGTDEFA
                     RIGAFGFVSGASSHADRLATIRKVYERYGVMIDTHTADGVKVAWERAADVPAGVPVLV
                     LETALPVKFAETIVEALGREPERPADLEGIEKLPQRVEVMAPDADAIKRFIAQRT"
BASE COUNT          749 a         1987 c         1650 g          697 t
ORIGIN      
        1 tcgagaagaa cgccccgctg ctcgagccct ggcagcgcga ggtggtgcgc atcgtgcgca
       61 agatcgcgca gtacttcttc ccgcagcgcc agacccaggt catgaacgaa ggctgggcca
      121 ccttctggca ctacaccctg ctcaaccacc tctacgacga gggtctgctc gccgacagct
      181 tcatgctgga attcctgcag tcgcacacca acgtcgtcgc gcagccgccc tacaacagcc
      241 gctggttcgg cgggatcaac ccctacgcgc tgggcttcgc gatgtggcgg gacatccgcc
      301 gcatctgcga ggaccccgac gaggaagacc gccgctggtt ccccgagatc gccggcagcg
      361 actggctgga aaccttcgat ttcgcgatgc gcaacttcaa ggacgagagc ttcgtcgcgc
      421 aatacctgtc gcccaaggtc atgcgcgact tccgcctgtt cgcggtgctc gacgacgacc
      481 acgaggacaa gctcgaagtc tccgcgatcc acgacgacgg gggcttccgc aagctgcgcg
      541 agatcctctc cgaccagtac aacctgggcg cgcgcgaacc caacgtgcag gtgtggaacg
      601 tcgacctgcg cggcgaccgc tcgctcaccc tgcgccacac ccccttccgc cgccgcccgc
      661 tcggcgacgg caccgacgaa gtcatgcgcc acctcgcccg cctgtggggc ttcaccgtgc
      721 gcctcgagcg tgcggacgac gacggcaagg tcgaactcct ccacgagatc cgcggcgaga
      781 ccacccgccc ccgcccgccc ggctgagcgc ctgtcgcccg aagcgacccg aaggccaccg
      841 caggggcgac gcgggtcgcg ctccggccgc caagattgcg cccgcgcgca ttgggcgcgc
      901 gcagccgagg acgtagaatc ccgactcccc caccggagtc cgtcatgttc agccccctgc
      961 gtccgatcta ccccgtcgcc ggcatccgcg agatcgagga caaactcatc ccgaacgccc
     1021 gcccgccgct gatggagcgc gccgggcgcg ccgccgcgca ggatgcggta cggctgatca
     1081 tggatcgccc cgggccgatc ctgatcgcct gcggccctgg caacaatggc ggcgacggct
     1141 tcgtgatggc gcgccagctc gcccaggccg ggcgcgaggt ggtggtcgcg ttctgcagcg
     1201 cggccgagcg tctgccggcc gaggcggcca aggcgcacgc cgactacctc gccgccggcg
     1261 gcagcatcgt ctccgacctc cccgccgcgc cggccaacgg ctgggccctg gtggtcgacg
     1321 cgctcttcgg gatcggcctc ggacgcccga tcgaagaccg ctacgcgagc tggatccaca
     1381 ccctcaacgc ccaaccctgc ccgcgcatgg cgctcgacgt cccgagcgga ctcgacgccg
     1441 acaccggcag cccgctcggc gccaccttcc gcgccaccca caccaccacc ttcatcgccc
     1501 tcaaacccgg cctgctcacc aacgacggcc ccgaccactg cggcgagatc agcgtgcagc
     1561 gcatcgagat cgacgcccct gcctggctgc cggcgcgcgg tcacgcgatc gcatcctcgc
     1621 tgttccgcca tctgctgcag ccgcgcccgc gcaacaccca caagggtctc tacggcgacg
     1681 ccgccatcct cggcggcaac gccggcatgg tcggcgccgc gctactcgcc ggccgcgccg
     1741 cgctctggct ggggacggga agggtctatg tcggcctgct cgaccccgcc ggccccgcgg
     1801 tcgacttcgc ccaccccgag ctcatgctgc gtcgcgccga gaccctgccc gagcgcctca
     1861 gcgcgctcgc catcggcccc ggcctgggca cccagggcgc gtccgccaac gtgctcgccg
     1921 acgccctcgc gcgcccgatc ccgctgctcc tcgacgccga cgcgctcaac ctcctctccg
     1981 ccgatgcggc gctgcgccgg gtgttgtgca cgcgcggcgc agccaccgtg ctcacccccc
     2041 accccgcaga ggccgcccgc ctgctcggca gcgacaccgc aagcgtgcag gccgaccggc
     2101 tgcgtgccgc gctcgaactc gcccgccgct accgtgcctt ggtcgtgctc aagggctgtg
     2161 gcagcatcgt cgccacaccc gacgagcgct ggttcatcaa cggcagcggc cactccggga
     2221 tggcaagcgc gggcatggga gacgtgctga gcggcctcgt caccggcctg ctcgcgcagg
     2281 gctggccgcc cgagtccgcg ctcatcgccg gcgtacacct gcacggtgcc gcagccgacc
     2341 gactcgcacg cgaaggcatc ggtccggtcg ggttgagcgc gagcgagacc atcgacgccg
     2401 cccgtggcgt cttcaacggc tggctgatcg aggcgcagcg cgagccgcgc aacaccggcc
     2461 cccattcccc gcggaccggg cgctgagcgc gccggtgcta gaatcgcagc atcccggcgg
     2521 cgccaccggg cgcagccacc cggcgcctgg cgcgtctgtg cctggccgcc gcctcgcccg
     2581 gcccgccgcc cttcccgcct aaccttgcag gtcctcccat gctctccgaa cagctccgcg
     2641 tcgaaatcca gcgcaacatc gcagcctcgc tcgccgagga catcggcacc ggcgacctca
     2701 ccgcgcgcct gatcgcaccg gacaccgagg cgcgcggccg cgtcatcacc cgcgaggacg
     2761 cggtcatctg cggcaccgcc tggttcgacg ccgcgttcgc cgcgctcagc ccggcggcgg
     2821 tggtgcactg gcacgtcaag gacggcgaca gcgtcaaggc ggggcagacc ctgtgcgaga
     2881 tcgccgcgcg cgcacgcgtg ctgctgaccg ccgagcgcac cgcgctcaac ttcctgcagc
     2941 tgctctcggg caccgccacc gtcacccgcc gcttcgtcga tgcggtggcc ggcacccgcg
     3001 ccaagatcgt cgacacccgc aagaccctgc cgggcctgcg cctggcgcag aagtacgcgg
     3061 tcgcgatcgg tggcggcacc aaccaccgcg tcggcctcta cgacggcatc ctggtcaagg
     3121 agaaccacat catcgcggcc ggcggcatcc gcgaggtggt cgagcaggca agtgccatcg
     3181 cgccctcgaa cgtgttcatc gaggtcgagg tggagacact ggggcaactg cgcgaggcgc
     3241 tcgatgccgg cgtgaagatg atcctgctcg acaacatgac gctcgagcag atgtgcgagg
     3301 cggtggctat caatgccggc cgcgccgaac tcgaggcctc gggtggcgtg aatctcgagc
     3361 gcgtgcgcgc gatcgccgag accggcgtcg accgcatctc gatcggcacc ctgaccaagg
     3421 acgtacgagc gctcgacctg tcgctgcgcc acgtcgagcg ctgagggcgc gggtccgccc
     3481 atggccgcca gcgcccccct gccccctgcc gcgcccgccg cttccgaccc gcccgccctg
     3541 cccgcctggc ccatggtgcc ggcctgctac ggctggctcg cgctcgacgc ccgcggcggc
     3601 tggcggctgc gcggcgagat catcacccac cccggactgc tcgccttcct cgacgcccac
     3661 tacaccggcg acgcgcacgg caactggggg gtcgataacg gcccgcagcg cgtctacgtg
     3721 gaactcgctg cggcaccgct gatcctgcgc ctgcatcccg acggccgcct ggtcgcccac
     3781 accggacgcg aggcgaccac gaccggaccg gtgctggtcg acgcggacgg ttgcgccttc
     3841 ctcgccaccg acctgggtcc ggcgatgatc gacgaccgcg acctcgcgct tttcgccgcc
     3901 gagctgcgcc agctcgaggg cacgcccgcc ggcgacgagg acctgctcgc actgatcgac
     3961 gggcagtccg gcccggcact gcgctggcgc gaggtcgacg tggccttctg cgcgcgtgcg
     4021 gcaatccccg ggcgcctcgg cttccagccc cacccgcaag ccatcggcac ctgatccgca
     4081 gcgccgtctt ccgtcgccgc aacgacgccc gcgctcggcg ctcgcatcag acacggcagg
     4141 tgttgacccc cacccggctt cacggctatg atccgcgccc ttgctcgatg cggccggcgc
     4201 cacggcgcag aacaccgcag cagcgaagcc ttggtggtga aattggtaga cacgctatct
     4261 tgagggggta gtggcgaaag ccgtgcgagt tcgagtctcg cccaaggcac cagattacgg
     4321 ccccgccgac gacggcggcg tccgacgaag aaaagcccgc gaatcgctcg cgggcttttc
     4381 ttcgtttacg tcccggcgtc cccgccggcc cgctcgccga cggggacccg gcgatcaggt
     4441 gcgctgggcg atgaagcgct tgatcgcgtc ggcgtcgggc gccatcacct cgacgcgctg
     4501 cggcagcttc tcgatgcctt cgaggtctgc gggacgctcc ggctcgcgcc caagggcctc
     4561 gacgatggtc tcggcgaatt tcaccggcag cgcggtctcc agcaccagca ccggcacgcc
     4621 cgcaggtacg tcggcggcgc gctcccaggc caccttgacg ccgtcggcgg tgtgggtgtc
     4681 gatcatcacg ccgtagcgct cgtacacctt gcggatggtc gccaggcgat cggcatggct
     4741 gctcgcgcca gacacgaagc cgaacgcgcc gatgcgggcg aattcgtccg tgccggacag
     4801 gtcgaaggcg cgcccggcgt cgacctcggc ccacagcgcg gccaccttgc tcgggtcgcg
     4861 gccgaccagg tcgaagacga agcgctcgaa gttcgaggcc ttggagatgt ccatcgacgg
     4921 gctggaggtc acgtgcgtct cggccgcctt gcgcgggcga tacacgccgc tgcggaagaa
     4981 ctcgtcgagc acgtcgttct cgttggtggc gaggatcagc ttcgccaccg gcaggcccat
     5041 ctggcgggcg atgtggccgg cgcagatgtt gccgaagttg ccc
//
LOCUS       DAOQBS010000172         4983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_17661_length_4983_cov_14.552963, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000172 DAOQBS010000000
VERSION     DAOQBS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17661_length_4983_cov_14.552963"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PKN25_14030"
     CDS             <1..395
                     /locus_tag="PKN25_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HNV92065.1"
                     /translation="CPRNCAEAGIKDVGVIGVDSGYELYVGGNGGIKTEVAQYLCKVG
                     SDEEVMEYAGAFLQLYREEGWYLERTVHWLDRVGLDYVKAKVVEDGENRRRLHARLLD
                     ALKDARDPWQTSREEEAVKQFIPIKVEA"
     gene            591..902
                     /gene="nirD"
                     /locus_tag="PKN25_14035"
     CDS             591..902
                     /gene="nirD"
                     /locus_tag="PKN25_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HNV92066.1"
                     /translation="MTDWKRICLLDDIPRLGSRVVRRPGADDIAVFRNADDEVFALHD
                     QCPHKGGPLSQGIVHGRKVTCPLHGWNFNLQDGQAVAPDVGACASFAVKVEGGEVWLA
                     T"
     gene            complement(1041..2228)
                     /locus_tag="PKN25_14040"
     CDS             complement(1041..2228)
                     /locus_tag="PKN25_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNV92067.1"
                     /translation="MPAPLVILILSLLLGLQPITTDLYLPALPALTASFGAVPAQAQL
                     TLTALLLAFGASQLFWGPLSDRIGRRRVLLIGLAGYTLAAVGSALAGSIEVMVFWRVL
                     QGAFMGAGTVCARAIVRDLYAPQEGARAMSKGLSGLGVAACLSPPLGGVLAEWFGWRA
                     TLAALTVMGAATLFVVARHFQETLHERHPEALQPAVLLRTARGIVGNRSFWTYASLCT
                     ASFGGLFCILSASSFVFINLLGTSPSFYGVLLLLLSAFYIGGTMLCRRMIQRFGVQRS
                     VMIGGALSLSGGGLMLAFALSGVSTVWTIMVPAFIFMLGHGVHQPCGQSGAIAPFPKA
                     AGAASAITGFLMMVVAFGAAYWVGANTDGSALPMIVGVAFCSAAVAFIAWVPVQRVRL
                     GAV"
     gene            2573..3787
                     /locus_tag="PKN25_14045"
     CDS             2573..3787
                     /locus_tag="PKN25_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HNV92068.1"
                     /translation="MDKKSFLQAGHTPTLIAAFLYFDLAFMVWVILGPLAVGIAADLG
                     LSHAQKGLIVATPVLAGAILRMIMGVVVDRLSPKKAAIIGQLVVIGALAYAWLVGVHS
                     FNEVLVLGVFLGVAGASFAAALPLASRWYPPEHQGTAMGIAGAGNSGTAFAALFAPGL
                     AMAFGWHNVFGLALIPLVIVLVAFCFMAKDAPDAPPPKRFAEYLAVLRDKDAWWFMFF
                     YSVTFGGFVGLASSLVIYFNTQYGLDPKMAGYFTAACVFAGSLVRPIGGNVADRIGGV
                     KSLTVMYILAAIFLAIVSVGLPQAWMALVAFVGAMLALGMGNGAVFQLVPQRFRREIG
                     VMTGLVGMAGGIGGFYLASSLGFARQLTGSYQAGFLIFAALAVFALVGLTAVKTRWRT
                     TWGAAHLTSAKI"
     gene            3877..>4983
                     /locus_tag="PKN25_14050"
     CDS             3877..>4983
                     /locus_tag="PKN25_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNV92069.1"
                     /translation="MKDLTPLEILLGHASRTGPRPANEDFVAAAMPEGDERAAKGVLL
                     AIADGVGGHAHGREAAEYSVRSLLADYFSTAHTWSVQKSLDTVLGAANRWLLAQSART
                     AETAGMATTLTALVLRGRRWHLAHVGDSRAYLWRDGALLRLSEDHTWPHPELSNVLRR
                     ALGLESRLLVDHDDGELALGDVFVLLTDGVWNTLGDEAIAALLARQAGQEKEREGGGR
                     AAVGVVTPKPVTRGTEAQGRAGRENGVGVSEEAGACARVAEAGLDPQAAADALVEAAL
                     RAGGNDNASALVACVRALPADNLRDRLAAGRRLPLPPRLAEGDTLDGLRVEAVLHVSR
                     LTVLYRVCRIEDSTAAIAACAAPTSPQAASTSPAA"
BASE COUNT          775 a         1701 c         1797 g          710 t
ORIGIN      
        1 gctgcccgcg caactgcgcc gaggccggca tcaaggacgt gggcgtgatc ggcgtcgatt
       61 ccggctacga gctgtatgtg ggcggcaacg gcggcatcaa gaccgaggtc gcgcagtatc
      121 tgtgcaaggt gggcagcgac gaggaggtca tggagtatgc cggcgccttc ctgcagctct
      181 accgcgagga gggctggtac ctcgagcgca cggtgcattg gctcgaccgc gtcggcctcg
      241 actacgtgaa ggccaaggtg gtcgaggacg gcgagaaccg ccgccgcctg cacgcgcgcc
      301 tgctcgacgc gctcaaggac gcgcgcgatc cgtggcagac cagccgcgag gaggaggcgg
      361 tgaagcagtt catcccgatc aaggtcgagg cctgagtttc cgggcgccgg aatcgcgact
      421 cgcgtcgctc ctacgaaaac cacggcgcct cgatgacgtc ccgtaggagc gacgccagtc
      481 gcgatcaccg cccccgaccg agcgcggcgc ggattccggg cgccggcatc gcgactggcg
      541 tcgctcctac aaataacaac cgaaccaagc ttccgaagaa ggaacccacc atgaccgact
      601 ggaaacgcat ctgcctgctc gacgacatcc cccgcctcgg ctcccgcgtc gtgcggcgcc
      661 ccggcgcgga cgacatcgcc gtcttccgca acgccgacga cgaggtcttc gcgctgcacg
      721 accaatgccc gcacaagggc ggcccgctct cgcaaggcat cgtgcacggc cgcaaggtga
      781 cctgtccgct gcacggctgg aacttcaacc tgcaggacgg ccaggcggtg gcgccggacg
      841 tgggggcgtg cgcgagcttc gcggtgaagg tggagggcgg cgaggtgtgg ctggcgacct
      901 aaggggcgac ccagcgggtg atctccactc cgcatcgatc gtcggccccg ccgctcccgg
      961 aggggcgagg ctgggtcgcc cccacccccg catcgatcct ccgcccgccg ctcccgcagg
     1021 ggcgaccggc cgcgtcgccc ctacacggcc cccagcctta cgcgctgcac cggcacccag
     1081 gcgatgaacg cgacggcggc gctgcagaag gccacgccga cgatcatcgg cagggcgctg
     1141 ccgtccgtgt tggcgcccac ccagtacgcg gcgccgaagg ccaccaccat catcaggaag
     1201 ccggtgatcg ccgacgcggc gccggccgcc ttcgggaaag gggcgatcgc gccgctctgg
     1261 ccgcagggct ggtgcacccc gtggccgagc atgaagatga aggccggcac catgatggtc
     1321 cacaccgtgc tcaccccgga gagcgcgaac gccagcatca ggccgccgcc gctcagcgac
     1381 agggccccgc cgatcatcac gctgcgctgc acgccgaagc gctggatcat gcgccggcac
     1441 agcatggtgc cgccgatgta gaaggccgac agcaggagca gcagcacgcc gtagaagctc
     1501 ggcgaggtgc cgagcaggtt gatgaagacg aaggacgacg ccgacaggat gcagaacagc
     1561 ccgccgaagg acgcggtgca cagcgaggcg taggtccaga agctgcggtt gccgacgatg
     1621 ccgcgcgcgg tgcgcagcag gacggcgggc tgcagcgcct cggggtggcg ttcgtgcagg
     1681 gtctcctgga agtggcgggc gaccacgaac agggtcgccg cgcccatcac cgtcagcgcc
     1741 gccagcgtgg cgcgccagcc gaaccattcg gccagcacgc cgccgagcgg cgggctcagg
     1801 caggcggcca cgcccaggcc cgacaagccc ttgctcatcg cgcgcgcccc ttcctgcggc
     1861 gcataaagat cacgcacgat tgcgcgcgcg cataccgtgc ccgcgcccat gaacgcgccc
     1921 tgcagcaccc gccagaacac cattacctcg atcgagccgg cgagtgcgct gccgaccgcc
     1981 gccagcgtgt agccggcgag gccgatcagc agtacccggc ggcgtccgat gcgatccgac
     2041 agcgggcccc agaacagttg cgatgcgccg aaggcgagca ggagcgcggt gagggtcagc
     2101 tgcgcctgcg ccgggaccgc gccgaagctg gcggtgagcg caggcagggc ggggaggtaa
     2161 aggtcggtgg tgatcggttg caggccgagc agcagggaca ggatgaggat gacgaggggg
     2221 gcgggcatgg tggtttttgt aaatcgagaa taaataaaat tatcgagaaa atagcatgaa
     2281 tgctgcccgc aggcgtgctt cctcgtcggc gcagggggac ttgcgcgcaa gccggcacgc
     2341 ccgcgtgcgc accgcttttg tgcaccgcac accaagctgg tgcgtcgcca ccgtcgagcg
     2401 caaagtccaa ctgactgatt tagaacgatt tcaaatgtgg cacgacgctt gctgagcaga
     2461 caccagcaca gcgtacgacc ccgccaatga cggcggggcg gtcgggcgaa gaggttcgcg
     2521 ctggagattc ccgcaccgat ggtgcgcttc aagcacgacc ctggagcccg acatggacaa
     2581 gaaatccttc ctgcaggccg gccacacgcc gacactgatc gccgccttcc tctacttcga
     2641 cctcgccttc atggtgtggg tgatcctcgg tccgctggcg gtcggcatcg ccgccgacct
     2701 cggcctgtcg cacgcgcaga agggcctcat cgtggccacg ccggtgctgg cgggagcgat
     2761 cctgcgcatg atcatgggcg tggtggtcga tcgcctgtcg ccgaagaagg cggcgatcat
     2821 cggccagctc gtcgtgatcg gggccctggc ctacgcctgg ctggtgggcg tgcattcgtt
     2881 caacgaggtg ctggtgctgg gggtcttcct cggcgtggcg ggcgcgtcct tcgcggcggc
     2941 gctgccgctg gcctcgcgct ggtatccgcc cgagcaccag ggcaccgcga tgggcatcgc
     3001 cggggccggc aactcgggca ccgcgttcgc ggcgctgttc gcgcccgggc tggcgatggc
     3061 cttcggctgg cacaacgtgt tcgggctggc gctgatcccg ctggtgatcg tgctggtggc
     3121 cttctgcttc atggccaagg acgcgcccga cgcaccgccg cccaagcgct tcgccgaata
     3181 cctcgcggtg ctccgggaca aggacgcgtg gtggttcatg ttcttctact cggtgacctt
     3241 cggcggcttc gtcggcctgg cctcgtcgct ggtgatctac ttcaacaccc agtacgggct
     3301 ggatccgaag atggcgggct acttcaccgc ggcctgcgtg ttcgccggct cgctggtgcg
     3361 cccgatcggc ggcaacgtgg ccgaccgcat cggcggcgtg aagtcgctca ccgtgatgta
     3421 catcctggcg gcgatcttcc tcgccatcgt cagcgtcggc ctgccccagg cgtggatggc
     3481 gctggtggcc ttcgtgggtg cgatgctggc gctgggcatg ggcaacggcg cggtgttcca
     3541 gctcgtgccg cagcgcttcc gtcgcgagat cggcgtgatg accggcctgg tcggcatggc
     3601 gggcggcatc ggcggcttct acctggcgtc ctcgctcggc ttcgcgcgcc agctcaccgg
     3661 cagctaccag gccggcttcc tgatcttcgc ggcgctggcg gtgttcgcgc tggtcgggct
     3721 caccgcggtg aagacgcgct ggcgcacgac ctggggcgcg gcgcacctga cctcggcgaa
     3781 gatctgaatg gccgaatggg gtcccgaacg aggtcaggtc ctgagtttga tcatcccgca
     3841 tccgttggct tcacgtcgag aaaggcgagt gagcagatga aagacctgac ccccctggag
     3901 atcctcctcg gccacgccag ccggaccggg ccgcgcccgg ccaacgagga cttcgttgcc
     3961 gcggcgatgc ccgagggcga cgagcgcgcg gccaagggcg tgctgctggc gatcgccgac
     4021 ggcgtcggtg gccacgcgca cggccgcgag gcggcggagt attccgtgcg cagcctgctc
     4081 gccgactact tctccacggc ccacacctgg agcgtgcaga agtcgctcga caccgtgctc
     4141 ggcgcggcca accgctggct gctcgcgcag tcggcgcgta ccgccgagac tgcgggcatg
     4201 gcgaccacgc tcaccgcgct ggtgctgcgc gggcggcgct ggcacctcgc gcacgtgggc
     4261 gactcgcgcg cctacctgtg gcgcgacggc gcgctgctgc gcctgagcga ggaccacacc
     4321 tggccgcacc cggagctgag caacgtgctg cgccgcgcgc tggggctgga gtcgcgcctg
     4381 ctggtggatc acgacgacgg cgagctggcg ctgggcgacg tcttcgtgct gctgaccgac
     4441 ggggtgtgga acacgctcgg cgacgaggcg atcgcggcgc tgctcgcgcg ccaggcgggg
     4501 caggagaagg aaagggaggg tgggggaagg gctgcggtgg gggtggtgac accgaaaccc
     4561 gtaacgcggg gaacggaggc gcagggaagg gcggggcgtg aaaatggcgt gggggtgtcc
     4621 gaggaggccg gagcgtgcgc gagggtagcg gaagccggcc tggacccgca ggccgccgcc
     4681 gatgccctcg tcgaagccgc cttgcgcgcc ggcggcaatg acaacgccag cgcgctggtg
     4741 gcctgcgtgc gcgcgctgcc cgccgacaac ctgcgcgacc gcctcgccgc cggccgccgc
     4801 ctgccgctgc cgccgcgcct ggccgagggc gacacgctcg acggcctgcg cgtggaggcg
     4861 gtgctgcacg tgtcgcggct gaccgtgctg tatcgggttt gccgcatcga ggactccacg
     4921 gccgcaatcg cggcttgcgc cgctcctacg tcgccgcagg ccgcgtcgac ttcgcccgcc
     4981 gcg
//
LOCUS       DAOQBS010000173         4978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_17680_length_4978_cov_14.384319, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000173 DAOQBS010000000
VERSION     DAOQBS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17680_length_4978_cov_14.384319"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PKN25_14055"
     CDS             complement(<1..625)
                     /locus_tag="PKN25_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV92070.1"
                     /translation="MNETLDTGAQPVVVACTGLSKQFREGAEAVRVLDGVELEVRRGE
                     RVAIVGSSGSGKSTLMHLLGGLDVPSAGRVRLMGQDFSTMSEAARGRLRNASLGFVYQ
                     FHHLLPEFSALENVAMPLYIRRVARAEADERAAQMLREVGLGHRLDHAPGELSGGERQ
                     RAAIARALVTQPACVLADEPTGNLDRRTAGAVFDLMLSLNERLATSFV"
     gene            complement(618..1874)
                     /locus_tag="PKN25_14060"
     CDS             complement(618..1874)
                     /locus_tag="PKN25_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNV92071.1"
                     /translation="MPYEILVGLRYTRSRKRAQGRNRFISFISLVSMFGIALGVAALI
                     VVLSVMNGFQEELRTRILGVASHVQVQSPEGGLMAWQQVADESLRNPSVRAAAPYVQE
                     QGMLSFDEGVRGTLVRGVIPSAEEKVADFGRYMQAGSFDALQAGRFGIVLGRDLAAAL
                     RVRLGEKVTLIAPQGLVTPAAVLPRVKQFEVVGIFEAGMYEYDSSLALVHIADAQALY
                     RLGDAVSGVRLKLDDLFAAPRVARELAMTISEPGLVVADWTRSHANFFRAVALEKTMM
                     TLILFLIVAVAAFNIVSTLVMAVQEKYADIAILRTLGASPVSIMAIFVLQGSVIGLVG
                     LAAGVAGGLAIAHNLDVVIPALETLTGATLWNKEIYYINELPSKVLGSDVSTIVTVSF
                     VLTLVAALYPSWRASKVNPAEALRYE"
     gene            2102..4021
                     /locus_tag="PKN25_14065"
     CDS             2102..4021
                     /locus_tag="PKN25_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HNV92072.1"
                     /translation="MLDRSTAAWLALILGLLLTAVLWRYVDAHLEERTRDRFATHATL
                     ARDNLLSRVQAYEQVLHGGAALFAASDHVGRSEWAAYVAALHLERSLPGIQGTGFTLM
                     LLPAQLEAHVAAVRAEGFPDYTVHPPGYRDRYSSIVYLEPFDGRNRRAFGYDMYSEPV
                     RREAMERARDTGMAALSGRVTLVQEFGTEVQPGFLIYLPVYAKAMPVASVEQRRAALL
                     GYVYAPFRAYDMMQAIFRDDLRDADIELHDLHESPQTLLYASAERNAAAHYVVTHAVE
                     VAGREWVVRFSSTPAFEADHASAEPALILYGGSALSLMLFAVMHSNARHRRRMRAAAS
                     HLAHSRDEFRTLVENVPGVVFRCQIEPPWRVMHISRGITALTGATPEDFMSGRLSFGD
                     FILHEDVPTIVAAIESARISHTNYEVEYRMRGRDRRVHWVSERGRVAVDADDEPLRLD
                     GVIIDITERKAAEEAIRNLAFVDTLTQLPNRRFLLDRLRQGLANARRHGRHGALLFID
                     IDHFKRVNDTYGHEAGDQLLCEIAARLRQVVREGDTVARLGGDEFVVMLEDLGDATAE
                     AGEKAALIAGNILAALNEPYAIGAVRHHSTPSIGIACFDGGEQRAEELLRRADEAMYR
                     AKAGGRNRLETLNAS"
     gene            complement(4018..>4978)
                     /locus_tag="PKN25_14070"
     CDS             complement(4018..>4978)
                     /locus_tag="PKN25_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HNV92073.1"
                     /translation="GCTFCSITEHEGRIIQSRSHASIIHEIEEIRDKSPDFKGHITDL
                     GGPTANMYRMACKSKAIEENCRRLSCVYPGICENLDTDHSSLVELYRKARAVPGIKRI
                     TIGSGLRYDLAVRSPEYVKELVTHHVSGLLKIAPEHTEENTLSKMMKPGIGAYEEFAR
                     MFEKYSKLAGKKQHLVPYFIAAHPGTTDEDMLNLALWLKAHDFRLDQVQTFLPTPMAL
                     ATTMYHSRKNPLKKVAADSEVVETARAGRIRKLHKAFLRWHDPENWPLLREALIEMGR
                     AELIGNGPHCLVPRQQPARLVAAKAGGGRKPGGCAKSRARRGA"
BASE COUNT          789 a         1767 c         1678 g          744 t
ORIGIN      
        1 tgacgaagct ggtcgccagg cgctcgttga gcgaaagcat gagatcgaac accgcaccgg
       61 ctgtgcggcg gtcgaggttg ccggtgggct cgtctgcgag cacgcaggcg ggctgtgtga
      121 cgagcgcgcg cgcgatcgcg gcgcgctggc gttcgccgcc ggagagctcg cccggagcgt
      181 ggtcgaggcg atggccgagg ccgacctcgc gcagcatctg cgcggcacgc tcgtccgcct
      241 cggcacgcgc gacgcggcgg atgtagagcg gcatcgcgac gttctcgagc gccgagaact
      301 ccggcagcag gtgatggaac tgatacacga agccgagcga ggcgttgcgc aggcgtccgc
      361 gcgcggcctc cgacatggtc gagaagtcct gccccatcag ccgcaccctg cccgcgctgg
      421 gcacgtcgag tccgccgagc aggtgcatca gggtgctctt gcccgagccg gacgagccca
      481 cgatcgcgac gcgctcgcca cgccgcacct cgagctcgac gccgtcgagc acgcgcaccg
      541 cctccgcgcc ctcgcgaaac tgtttggaca gcccggtgca ggcgacgacc acgggttgcg
      601 cgccggtatc cagggtttca ttcatagcgc agcgcctccg caggattcac cttcgaggcg
      661 cgccagctcg ggtagagcgc ggccaccagg gtcagcacga aggaaacggt gacgatggtg
      721 ctcacgtccg agcccaggac cttcgaaggc agctcgttga tgtaatagat ttccttgttc
      781 cacagcgtcg ccccggtgag cgtctcgagc gcggggatca ccacgtcgag gttgtgcgcg
      841 atcgccagcc cgccggccac gcccgcggcc aggcccacca ggccgatcac cgagccctgc
      901 agcacgaaga tcgccatgat cgagaccggg ctcgcgccca gcgtgcgcag gatggcgatg
      961 tcggcgtact tctcctgcac cgccatcacc agcgtggaga cgatgttgaa ggccgcgacc
     1021 gccacgatca ggaagaggat cagcgtcatc atggtcttct cgagcgcgac cgcgcggaag
     1081 aagttggcgt ggctgcgcgt ccagtcggcg acgaccaaac ccggctcgct gatcgtcatc
     1141 gccagctcgc gcgccacccg cggcgcggcg aagaggtcgt cgagcttcag gcgcacgccg
     1201 ctcaccgcgt cgcccagccg gtacagcgcc tgggcgtcgg cgatgtgcac gagcgcgagg
     1261 ctggagtcgt actcgtacat gccggcctcg aagatgccca ccacctcgaa ctgcttgacg
     1321 cgcggcagca ccgccgccgg cgtcaccagg ccctgcggcg cgatcagcgt gaccttctcg
     1381 cccaggcgca cgcgcagcgc ggcggcgagg tcgcgtccga gcacgatgcc gaagcggccc
     1441 gcctgcagtg catcgaagct gccggcctgc atgtagcggc cgaaatcggc gaccttttcc
     1501 tccgcggacg ggatcacccc gcgcaccagc gttccgcgca cgccctcgtc gaaggagagc
     1561 atgccctgct cctgcacgta gggcgccgcg gcgcgcaccg aggggttgcg cagcgactcg
     1621 tcggcgacct gctgccaggc catgagcccg ccctccgggc tctgcacctg cacgtgcgag
     1681 gcgacaccga ggatgcgggt gcgcaactcc tcctggaagc cgttcatcac cgaaagcacc
     1741 acgatcagcg ccgcgacgcc cagcgcgatg ccgaacatcg ataccaggga gatgaaggag
     1801 atgaagcggt tgcgcccctg ggcgcgcttg cgcgagcggg tgtagcgcag gccgacgagg
     1861 atttcgtatg gcattgcttg ctgtcgacga tggccgggaa ggacaagggc ggcattgtgc
     1921 ccgctggcgg tcgcgcgcgc gacctcgaaa cgcatgcgga tgtaagcccg gccgcggcgg
     1981 cggtcccacc tgcgggctgg ccgctccgca cgattcgtgg aacactcgcg aacaggccac
     2041 cccctcaccc ctcccgccca tgacgcagga cgcaggatcc gcgcaagttc gcgccgccgc
     2101 catgctcgac cgcagcacgg ccgcctggct cgcgctcatc ctgggcctgc tcctgacggc
     2161 ggtgctgtgg agatacgtcg acgcccatct cgaggagcgg acgcgcgacc gcttcgccac
     2221 gcacgccacg ctggcccgcg acaacctgct ctcgcgcgtg caggcctacg agcaggtgct
     2281 gcatggcggc gccgcgctgt tcgccgcgag cgaccacgtc ggccggtccg agtgggcggc
     2341 ctatgtcgca gccctccacc tcgagcgctc gctgcccggt atccagggca ccggcttcac
     2401 cctcatgctg ctcccggcgc agctggaggc ccacgtcgcg gcggtgcgcg ccgaaggctt
     2461 ccccgactac acggtacatc cgcccgggta tcgcgaccgc tacagcagca ttgtctacct
     2521 ggagcccttc gacgggcgca accggcgcgc cttcggctac gacatgtatt ccgagccggt
     2581 gcgccgcgag gccatggaac gcgcgcgcga cacgggaatg gccgcgctca gcggcagggt
     2641 caccctggtt caggagttcg gcaccgaggt gcagcccggc ttcctgatct acctgcccgt
     2701 gtacgcgaag gccatgccgg tcgcgagcgt ggagcagcgc cgcgccgcct tgctgggcta
     2761 cgtctacgcg ccgttccgcg cgtacgacat gatgcaggcc atcttccgcg acgacctgcg
     2821 cgacgccgac atcgaactgc acgacctgca cgaatcgccg cagaccctgc tctacgcctc
     2881 ggccgagcgc aatgccgccg cgcactacgt cgtcacccac gcggtcgagg tcgcagggcg
     2941 cgaatgggtg gtccgcttca gcagcacgcc ggccttcgag gccgaccacg cgagcgcgga
     3001 acccgccctg atcctctacg gtggcagcgc tctcagcctg atgctgttcg cggtgatgca
     3061 cagcaacgcc cgccaccgcc gccgcatgcg tgccgccgcg tcgcacctgg cgcacagccg
     3121 cgacgagttc cgcaccctgg tcgagaacgt gcccggcgtc gtcttccgct gccagatcga
     3181 accgccctgg agggtgatgc acatcagccg cgggatcacc gcgctcaccg gggcgacacc
     3241 cgaggatttc atgtccggtc gattgtcgtt cggagacttc atcctgcatg aggacgtccc
     3301 cacgatcgtc gccgcgatcg agagcgcacg catcagtcac accaactacg aggtggaata
     3361 ccgcatgcgc gggcgcgacc ggcgcgtcca ttgggtcagc gagcgcggcc gcgtggcggt
     3421 cgacgccgat gatgaaccgc tccggctcga cggggtgatc atcgacatca ccgaacgcaa
     3481 ggccgccgag gaagcgatcc gcaatctcgc cttcgtcgac acgctgaccc agctccccaa
     3541 ccgccgtttc ctgctcgacc gcctgcgcca gggcctggcc aacgcgcgcc gccacggccg
     3601 ccacggggcg ctgctgttca tcgatatcga ccacttcaag cgcgtaaacg acacctacgg
     3661 ccacgaggcc ggcgaccagc tgctgtgcga gatcgcggcg cgcctgcgcc aggtggtgcg
     3721 cgagggcgac accgtggcgc gcctcggtgg agacgagttc gtggtgatgc tggaggatct
     3781 gggcgacgcc acagccgagg cgggcgaaaa ggcagccctg atcgccggca acatcctcgc
     3841 cgcactcaac gagccatacg caatcggggc agtgcgtcat cacagcacgc ccagcatcgg
     3901 catcgcctgc ttcgacggcg gcgagcagcg cgccgaagaa ctgctgcggc gggccgacga
     3961 ggcgatgtac cgcgccaagg ccggcggacg caaccggctc gagacgctga acgcctccta
     4021 ggccccgcgc ctcgcccgcg atttcgcgca accacccggc ttgcgcccgc ccccggcctt
     4081 cgccgccacc agccgtgccg gctgctggcg cggcaccagg cagtgcggac cgttgccgat
     4141 cagctcggcg cgccccatct cgatcagcgc ctcgcgcagc agcggccagt tctccgggtc
     4201 gtgccagcgc aggaaggcct tgtgcagctt gcggatgcgg ccggcgcgcg cggtctccac
     4261 cacctcggaa tcggccgcga ccttcttcag cgggttcttg cgcgagtggt acatcgtggt
     4321 cgccagcgcc atcggcgtgg gcaggaaggt ctgcacctgg tcgaggcgga agtcgtgcgc
     4381 cttgagccac agcgccaggt tgagcatgtc ctcgtcggtg gtgcccggat gggcggcgat
     4441 gaagtacggc accaggtgct gcttcttgcc cgcgagcttc gagtacttct cgaacatgcg
     4501 cgcgaactcc tcgtaggcgc cgatgcccgg cttcatcatc ttggagagcg tgttctcctc
     4561 ggtgtgctcg ggcgcgatct tgagcaggcc gctgacgtgg tgggtgacga gctccttcac
     4621 gtattccggc gagcgcaccg cgaggtcgta gcgcaggccc gagccgatgg tgatgcgctt
     4681 gatccctggc accgcgcgcg ccttgcggta gagctcgacg agcgaggaat ggtcggtgtc
     4741 gaggttctcg cagatgcccg gatacacgca cgagagtcgc cggcagttct cctcgatcgc
     4801 cttgctcttg cacgccatgc ggtacatgtt ggcggtgggc ccgccgaggt cggtgatgtg
     4861 gcccttgaag tcgggcgact tgtcgcggat ctcctcgatc tcgtgaatga tggacgcgtg
     4921 cgagcggctc tggatgatgc ggccctcgtg ctcggtgatc gagcagaagg tgcagccg
//
LOCUS       DAOQBS010000174         4944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_17851_length_4944_cov_14.708938, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000174 DAOQBS010000000
VERSION     DAOQBS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17851_length_4944_cov_14.708938"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1871)
                     /locus_tag="PKN25_14075"
     CDS             complement(132..1871)
                     /locus_tag="PKN25_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNV92074.1"
                     /translation="MQQALARHHSIFLLILRRLRAPLILLIVLFAIAVLGLTLVPGPV
                     VDGETSYLSFFHAFYFISYTATTIGFGEIPYTFSDQQRLWVIVSIYLSVIGWAYTLGS
                     VFSLLADRSLRQAIAMQGFVRAVRRLREPFYLVCGYGETGRLICDALDRMGLRVVVIE
                     VDETKLGELDLHSYSADVPALCADAANPETLQFGGLTHASCIGVIALTNDDATNLAIA
                     IAARLLAPKVPALCRAEHTATSANMTSFGTRHILNPFERFSETLALSLHAPKASQLFD
                     WLTGLPGSHVEQRRDPPRGNWIVCGHGRFGRLLVDAMDSEAVPVTIIDIDPKPDGIHR
                     WVQGDGTGAASLLEAGVREATGIVCGTSSDVDNLSIAVTARELNQELFVILRQNHESN
                     RALFEAFESDITVVPSRVIAHECIAILSTPLLAPFLAEIRRRDEEWCGALLHRLTRHL
                     GWRVPRIRSQRVNLSSAPALYRRLMRGETITLERLLRSPADRSMALDCAVLYLERDDD
                     DHRMTPAADEKLRPGDELLFAGTRRALEDVALIFANEHTLEYILTGRDLPGGRVWEML
                     AQRKHGKRSPQLP"
     gene            complement(1897..2280)
                     /locus_tag="PKN25_14080"
     CDS             complement(1897..2280)
                     /locus_tag="PKN25_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HNV92075.1"
                     /translation="MNLEKVIFGFFIVLAATLNFGFFVGDIDNPVHHDVYELFAALVV
                     SLIATVLKFGDRTQIGAVHLSTSLVADLQLVAAAMVWGYSAHVAGAGMTPEMTARVVS
                     LSGGALFANVVSVIILIAETIMQRR"
     gene            complement(2489..2878)
                     /locus_tag="PKN25_14085"
     CDS             complement(2489..2878)
                     /locus_tag="PKN25_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HNV92076.1"
                     /translation="MIPSTPCPCGSGRAFGACCGPVLAGERPAPTAEALMRSRYTAFT
                     LRDTAYLLASWHPSTRPQDLALDEQPPPKWLGLDVRAHRPLADTRAEVEFLARCRIGG
                     RARRLHETSRFVKEDGRWYYVDGDLHD"
     gene            complement(2979..>4944)
                     /gene="acnA"
                     /locus_tag="PKN25_14090"
     CDS             complement(2979..>4944)
                     /gene="acnA"
                     /locus_tag="PKN25_14090"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNV92077.1"
                     /translation="IGVELRGSLPGGTTATDLVLAVTEMLRREKVVGKFVEFFGEGTA
                     SLSVTDRATLANMAPEYGATMGFFPVDAKTIDYMRATGREEAECALMEGYFRAQGLFG
                     VPRAGEIDYTRSLMLDLATIEPALAGPKRPQDRIALPAMRHEFERLFSAPVSENGFGR
                     EAARLDDRFATGREDVDLGHGDVLIAAITSCTNTSNPAVMIAAGLLAQKAAARGLRVK
                     PHIKTSLAPGSRVVTDYLGKAGLLEPLATLGFALAGYGCTTCIGNAGDLAPDFNAAIV
                     EHDLVVAAVLSGNRNFEARIHPNIRANYLASPPLVVAFALSGRVNIDLDTEPLGTGSD
                     GRPVYLRELWPSSDEIAAALPFATDPASYRRLYADFTRDHDLWNAIPAPTGQTYDWPP
                     STYIACPPFFAGFGMTPPPASDIHDARALLLLGDSVTTDHISPAGSFRETSPAGRWLR
                     GQGVEKTDFNSYGSRRGNHDVMVRGTFANVRVKNLMLPPNPDGSRVEGGYTLLDGEQT
                     TVFEAASAWIARGVPTIVFAGEEYGTGSSRDWAAKGTALLGVKAVVARSFERIHRSNL
                     VGMGVLPLQFIGEDSWQALGLDGSEKFTLEGIAAGLAPQRTLTLQIERADGSRQSVPL
                     LCRIDTPIEVEYYRHGGILPYVLRAILGGS"
BASE COUNT          738 a         1556 c         1920 g          730 t
ORIGIN      
        1 cgggtgtcgt tcgccggcga ggcggtggaa ctcgtggagg aaggtgcggg ctagcaggtc
       61 tcgcagacgc ggcgcacgtc gcgatcggcg ctcgagcggc ggggccgtgc cccggcaacg
      121 tggggagcgg ctcagggcag ctgcggcgag cgtttcccgt gcttgcgctg cgcgagcatt
      181 tcccagaccc gtccgccggg caggtcgcgg ccggtgagga tgtattcgag ggtgtgctcg
      241 ttggcgaaga tcagggcgac atcctcgagc gcgcggcggg tgccggcgaa gagcaattcg
      301 tcgccggggc ggagcttctc gtcggcggcg ggcgtcatcc ggtggtcgtc gtcgtcgcgc
      361 tcgaggtaga gcaccgcgca gtcgagcgcc atcgagcggt cggcgggcga gcgcagcagg
      421 cgttcgagcg tgatcgtctc gccgcgcatc aggcggcgat acagcgccgg cgcgctcgac
      481 aggttgacgc gctggctgcg gatccgcgga accctccagc cgaggtggcg ggtcaggcga
      541 tgcagcagtg cgccgcacca ctcctcgtca cggcggcgga tttcggccag gaagggcgcg
      601 agcagcggtg tgctcaggat cgcgatgcat tcgtgcgcga tcacccggct cgggaccacg
      661 gtgatgtcgg attcgaaggc ctcgaagagc gcgcggttgg attcgtggtt ctggcgcagg
      721 atcacgaaga gctcctggtt gagctcgcgt gccgtcaccg cgatcgaaag gttgtccacg
      781 tccgagctgg tgccgcacac gatcccggtc gcctcgcgca cgccggcctc gagcagggat
      841 gcggcaccgg tgccgtcgcc ctgcacccag cggtggatgc cgtcgggctt ggggtcgatg
      901 tcgatgatgg tcaccggcac tgcctcggag tccatcgcat cgaccagcag gcgaccgaag
      961 cgcccgtggc cgcagacgat ccagttgccg cgcggcggat cgcggcgctg ctcgacgtgg
     1021 ctgccgggca ggccggtgag ccagtcgaag agctgcgagg ccttgggcgc gtgcagcgag
     1081 agtgcgagcg tttcgctgaa gcgctcgaag gggttgagga tgtggcgggt gccgaaggag
     1141 gtcatgttgg ccgaggtcgc ggtgtgctcg gcgcggcaca gtgcgggcac cttcggcgcc
     1201 agcaggcgcg cggcgatcgc gatcgccagg ttggtggcgt cgtcgttggt gagcgcgatg
     1261 acgccgatgc agctcgcgtg ggtgaggccg ccgaactgga gcgtctcggg gtttgcggcg
     1321 tccgcgcaca gcgcgggcac gtcggccgaa tagctgtgca gatcgagctc gccgagcttg
     1381 gtctcgtcga cctcgatcac cacgacgcgc aggcccatgc ggtcgagcgc gtcgcagatc
     1441 agacggccgg tctcgccgta gccgcacacc aggtagaagg gctcgcgcag ccgccgcacc
     1501 gcgcgcacga aaccctgcat cgcgatcgcc tggcgcaggc tgcggtcggc gagcaggctg
     1561 aacaccgagc ccagcgtata ggcccagccg atcaccgaca ggtagatgct gacgatcacc
     1621 cacagccgct gctggtcgga gaaggtgtag ggaatctcgc cgaaaccgat cgtggtcgcg
     1681 gtgtagctga tgaagtagaa ggcgtggaag aagctgaggt agctcgtctc gccgtcgacg
     1741 accgggccgg gcaccagggt gaggccgagc accgcgatcg cgaacagcac gatcagcagg
     1801 atcagcggtg cgcgcaggcg acgcaggatc agcaggaaga tgctgtggtg gcgggcgagg
     1861 gcctgctgca cggcgcgggt ctcggcgagg tggcgttcag cggcgctgca tgatggtctc
     1921 ggcgatcagg atgatcaccg acaccacgtt ggcgaagagc gcgccgcccg agagcgagac
     1981 cacgcgcgcg gtcatctccg gcgtcatccc cgcgcccgcg acgtgcgccg agtagcccca
     2041 caccatcgcg gcggcgacca gctgcaggtc ggccaccagg ctggtggaca gatgcaccgc
     2101 gccgatctgg gtgcggtcgc cgaacttgag cacggtggcg atcaggctga ccaccagcgc
     2161 ggcgaagagc tcgtagacgt cgtggtggac cggattgtcg atgtcgccga cgaagaagcc
     2221 gaagttgagc gtggccgcca gcacgatgaa gaaaccgaag atgacttttt cgaggttcat
     2281 gatcttccgt tgggtgcgcg cgatgacgat ggggcaatta tgcgcgcgcc ggacctgcga
     2341 tgtcgcgcag cgccgacagc aggccgggtg gccgcgcgga ttgcggggcg ttttggtctt
     2401 tgtgggagcg acgcaagtcg cgatcgggcg gttcgctcag gcgccggcgg tgatcccggg
     2461 ccgaggccgg ctggcacgag ggcgagcctc agtcgtggag gtcgccgtcg acgtaatacc
     2521 agcgcccgtc ctccttcacg aagcggctgg tctcatgcag gcgccgggcg cggccgccga
     2581 tgcggcaacg ggcgaggaat tcgacctccg cgcgggtgtc ggcgagcggg cggtgggcac
     2641 gcacgtcgag gccgagccat ttcggcggcg gctgctcgtc gagcgccaag tcctgcgggc
     2701 gggtggaggg atgccagctc gcgagcaggt aggcggtgtc gcgcaaggtg aaggccgtgt
     2761 agcgcgagcg catcagcgcc tcggcggtgg gcgccgggcg ttcgcccgcg agcaccggcc
     2821 cgcagcacgc cccgaatgcg cgaccggagc cgcaggggca aggggtggag gggatcatgg
     2881 ggggcgagta tagcgggccg aggggagatg cggacgcggg gccggagcgt gctgcggggg
     2941 gggctgcgac gacgcaggtt gcgatccggc cgcgcggctc agcttccgcc gaggatcgcg
     3001 cgcagcacgt agggcaggat gccgccgtgg cggtagtact cgacctcgat cggggtgtcg
     3061 atacggcaca gcaggggcac ggactgcctc gagccgtcgg cgcgctcgat ctgcagcgtg
     3121 agcgtccgct gcggcgccag accggcggca atgccttcca gcgtgaactt ctcgctccca
     3181 tccagcccca gcgcctgcca gctgtcctcg ccgatgaact gcagcggcag cacgcccatg
     3241 ccgaccaggt tggagcggtg gatgcgctcg aagctcctcg ccaccaccgc cttcaccccg
     3301 agcagcgcgg tgcccttggc agcccagtcg cgcgacgagc cggtgccgta ttcctcgccc
     3361 gcgaacacga tggtcggcac gccgcgcgcg atccatgcgc tcgccgcctc gaacaccgtg
     3421 gtctgctcgc cgtcgagcag ggtgtagccg ccttcgacgc ggctgccgtc ggggttgggc
     3481 ggcagcatca ggttcttcac gcgcacgttg gcgaaggtgc cgcgcaccat cacgtcgtgg
     3541 ttgccgcgac gcgagccgta ggagttgaag tccgtcttct ccacaccctg cccacgcagc
     3601 cagcgcccgg ccgggctggt ctcgcggaag gaacctgcgg gcgagatgtg gtcggtggtc
     3661 accgagtcgc cgagcagcag cagtgcgcgt gcgtcgtgaa tgtcgctcgc cggcgggggc
     3721 gtcatgccga agcccgcgaa gaagggcggg caggcgatgt aggtggaggg cggccagtcg
     3781 taggtctggc cggtgggcgc ggggatcgcg ttccacaggt cgtggtcgcg cgtgaagtcg
     3841 gcgtagagcc ggcggtagct ggccggatcg gtggcgaagg gtagtgcggc ggcgatctcg
     3901 tccgagctcg gccagagctc gcgcagatac accggccgac cgtcgctgcc ggtgccgagc
     3961 ggctcggtgt cgaggtcgat gttgacccgc ccggagagcg cgaaggccac caccagcggc
     4021 ggcgaggcga ggtagttggc gcggatgttg gggtggatgc gggcctcgaa attgcggttg
     4081 cccgacagca cggcggccac cacgaggtcg tgctcgacga tcgccgcgtt gaagtcgggg
     4141 gcgagatcgc cggcgttgcc gatgcaggtg gtgcaaccgt agccggcgag cgcgaagccg
     4201 agcgtggcga gcggctcgag caggccggcc ttgccgaggt agtcggtgac cacccgcgag
     4261 cccggcgcga gcgaggtctt gatgtgtggc ttcacgcgca gcccgcgcgc ggccgccttc
     4321 tgcgcgagca ggccggcggc gatcatcacc gccgggttgc tggtgttggt gcacgaggtg
     4381 atggcggcga tcagcacgtc gccgtggccg aggtccacat cctctcgtcc ggtggcgaag
     4441 cggtcgtcga gccgggcggc ctcgcggccg aagccgttct cggacactgg cgcggagaac
     4501 aggcgctcga actcgtggcg catggcgggc agggcgatgc ggtcctgcgg gcgcttgggg
     4561 ccggcgagcg cgggctcgat cgtggcgagg tccagcatca ggctgcgcgt gtagtcgatc
     4621 tcgcccgcgc gcggcacgcc gaacaggcct tgcgcgcgga agtagccttc catcagcgcg
     4681 cattcggcct cttcgcggcc ggtggcgcgc atgtagtcga tggtcttcgc gtccaccggg
     4741 aagaagccca tggtggcgcc atactccggc gccatgttgg cgagggtggc gcgatcggtg
     4801 accgacaggc tggcggtgcc ctcgccgaag aactcgacga acttgcccac caccttctcg
     4861 cgccgcagca tctcggtgac ggcgaggacc aggtcggtgg cggtggtgcc gccgggcagg
     4921 ctgccgcgca gctccacgcc gatc
//
LOCUS       DAOQBS010000175         4895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_18113_length_4895_cov_12.089463, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000175 DAOQBS010000000
VERSION     DAOQBS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18113_length_4895_cov_12.089463"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2096
                     /locus_tag="PKN25_14095"
     CDS             <1..2096
                     /locus_tag="PKN25_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNV92078.1"
                     /translation="QYVQGLLRMSENLRLMLGANRGFRVHPEGPPEVQQLAVATNELA
                     SQRDVLLQDVEAQIGRAKASVELEKDRLAALMSELTQSVVVCNLDGRVLLYNNRARAQ
                     FRALSDAPGVAGGGELIGLGRSIYGVFERNLIAHALDTIQQRLRRGAAQAQANFVTTT
                     RAGQLLRVQMAPVLSLAAPALVAAESTAVAAGSVTGVQQNAAAEGDGAGNAGGGASPA
                     APEVAAAAVTSERTLTGFILMLDNITRNFETESRRDQMLHNLTEGNRASLANVRAAAD
                     MLEFEDLTDEMRTRFRKVVRDEVQAMSQRLDHTANEFADSLKARWPLEEMLGADLIAA
                     AQRRIEARIGLPTKLEEVDESLWVKVDSFSLIQAITYLASRLSDEFEVREVRFRLSAA
                     GRLVHLDLIWSGQAMSTETVMSWELEPMKIEDESSPLTVRDVVDRHDGEMWLEREKVR
                     HRAFFRILLPAATPQEQAEAETYLRGESRPEYYDFDLFAWSEKSHELDDRLLSELSYT
                     VFDTETTGLNPSQGDEIIQVGATRILNGKLLRAESFEQLVDPERPLAPESAKIHGITS
                     EMLRGQPTIDKVLPAFHAFAADTVLIAHNAAFDMRFLQLKEEQTGLRFDHPVIDTLLL
                     SAVVHPNQESHRLEAIAERLGLTIVGRHTALGDAIVTAEVFLKLIPLLAEKGIRTLRD
                     AREAAEKTYYARVKY"
     gene            2134..4179
                     /locus_tag="PKN25_14100"
     CDS             2134..4179
                     /locus_tag="PKN25_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HNV92079.1"
                     /translation="MRRNPGFARQLRRYYGIYTLGFALFVVLLAIGESLGLSQQLIGH
                     VFLFVTIAIYATIGVLSRTSDVTEYYVAGRRVPALFNGMATAADWMSAASFIGLAGTL
                     YFSGFEGLAFVTGWTGGFVLVALLLAPYLRKFGQYTIPDFLGARYQGNVARLVGLAAA
                     VLASFVYLVAQIYGVGLVTSRFVSVEFEIGLFIGLAGILVCSFLGGMRAVTWTQVAQY
                     VILIIAYLVPVVILSYKLTGIPIPQAVYGTVLQQISAREDELLEAPTERAVRDLYSAR
                     ARDYADKIAALPASLEDERRRMIDELNRMRLDSAPARDIALTERALRDLPRTPAEARE
                     AWQRARAEALERARPPPRHAEAHPGATASESATARNNFLALMFVLMVGTAALPHILMR
                     YYTTPGVMEARRSVLWSLFFIFLLYVTAPAYAVFAKWEVYNNLVGSNIAILPDWVASW
                     GRVGLVRIEDLNGDGILQLAELRLNTDVIVLATPEIAGLPYVVSGLVAAGGLAAALST
                     ADGLLLTISNALSHDLYYKVINPKASAHRRLVISKSQLLVVAVVAAWVASMRPDNILF
                     MVGLAFSIGGSAFFPALVLGIFWKRANRPGAVAGMLAGLAVTVFYVVRTHPFFGGAMA
                     NAWFEINPISAGVFGVPLGFATIVAVSLLTKPPPQEIQDLVDYVRYPDIPGQSRPHL"
     gene            4250..>4895
                     /locus_tag="PKN25_14105"
     CDS             4250..>4895
                     /locus_tag="PKN25_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNV92080.1"
                     /translation="MRFDKLTTKFQQALADAQSIAVGNDQQFIEPLHVLQALLEQDDG
                     GSVSLLQRAGVNVPPLKNAVAKAVGRLPKVEGHGGEVSIGRDLNNLLNVTDREAQKRG
                     DQFIASEMFLLALADDKGEAGRLLKENGLARKALEAAIEAVRGGANVGSQDAEGQRES
                     LSKYCLDLTERARSGKLDPVIGRDDEIRRAIQILQRRTKNNPVLIGEPGVGKTAI"
BASE COUNT          757 a         1752 c         1631 g          755 t
ORIGIN      
        1 gccagtacgt ccagggcctg ctgcgcatgt cggagaacct gcgcctgatg ctgggggcca
       61 accgcggttt ccgcgtgcac cccgaggggc cgcccgaggt gcagcagctc gcggtcgcca
      121 ccaacgagct cgcgagccag cgcgacgtgc tgctgcagga cgtggaggcg cagatcgggc
      181 gcgccaaggc ttcggtcgag ctcgagaagg accgcctcgc cgcgctgatg tccgagctca
      241 cccaaagcgt ggtggtgtgc aacctcgacg gccgcgtgct gctctacaac aaccgtgcgc
      301 gcgcccagtt ccgcgcgctc tcggacgcgc ccggcgtggc cggcggcggc gagctgatcg
      361 ggctggggcg ctccatctat ggcgtcttcg agcgcaacct gatcgcgcac gcgctcgaca
      421 ccatccagca gcgcctgcgt cgcggtgccg cccaggcaca ggccaacttc gtcaccacca
      481 cgcgtgccgg ccagctgctg cgcgtgcaga tggcgccggt gctgtcgctg gccgcgccgg
      541 cgcttgtggc ggcggagtcg acagccgtcg ccgcagggag cgtgaccggt gtgcagcaga
      601 atgcggcagc ggagggcgac ggcgctggca atgcaggggg cggcgcgtcc cccgcagcgc
      661 ccgaggtcgc cgcggcagcg gtcacgtccg agcgcacgct caccggtttc atcctgatgc
      721 tcgacaacat cacgcggaac ttcgagaccg agtcgcgccg cgaccagatg ctgcacaacc
      781 tcaccgaggg caaccgcgcc tcgctcgcca acgtgcgcgc ggcggccgac atgctcgagt
      841 tcgaggacct caccgacgag atgcggacgc gtttccgcaa ggtggtgcgc gacgaggtgc
      901 aggccatgag ccagcgcctc gaccacacgg cgaacgagtt cgccgactcg ctgaaggcgc
      961 gctggccgct cgaggagatg ctcggcgccg acctgatcgc cgcggcgcag cgccgcatcg
     1021 aggcccgcat cggcctgccc accaagctcg aggaggtcga tgaatccctg tgggtgaagg
     1081 tcgacagctt ctcgctgatc caggccatca cctacctcgc cagccggctg tcggacgaat
     1141 tcgaggtgcg cgaggtgcgc ttccgcctga gcgcggccgg gcgcctcgtg cacctcgacc
     1201 tgatctggtc ggggcaggcg atgagcaccg agacggtgat gagctgggag ctcgaaccga
     1261 tgaagatcga ggacgagagc agcccgctca ccgtgcgcga cgtggtcgac cgccacgacg
     1321 gcgagatgtg gctcgagcgc gagaaggtgc gccaccgtgc cttcttccgc atcctgctgc
     1381 cggcggcgac gccgcaggag caggccgagg ccgagaccta cctgcgcggc gagagccgtc
     1441 ccgagtacta cgacttcgat ctcttcgcgt ggtcggagaa gtcgcacgaa ctcgacgacc
     1501 gcctgctctc cgagctcagc tacacggtgt tcgacaccga gaccaccggg ctcaacccct
     1561 cgcagggcga cgagatcatc caggtcggcg ccacccgcat cctcaacggc aagctgctgc
     1621 gcgcggaatc cttcgagcag ctggtggacc cggagcgtcc gctcgccccc gagtcggcca
     1681 agatccatgg catcacctcg gagatgctgc gcggccagcc caccatcgac aaggtgctgc
     1741 ccgccttcca cgccttcgcc gccgacaccg tgctgatcgc gcacaacgcc gccttcgaca
     1801 tgcggttcct gcagctcaag gaagagcaga ccggcctgcg cttcgaccat ccggtgatcg
     1861 acaccctgct gctgtcggca gtggtgcatc ccaaccagga gtcgcaccgc ctggaggcga
     1921 tcgccgagcg cctgggcctg accatcgtcg gccgtcacac cgcgctcggc gacgcgatcg
     1981 tgaccgccga ggtctttctc aagctgatcc cgctgctcgc cgagaagggc atccgcaccc
     2041 tgcgcgacgc gcgcgaggcc gccgagaaga cctactacgc gcgggtcaag tactgatgac
     2101 cggccgccgc aggccgcgcg gagagcattc cccttgaggc gcaaccccgg cttcgcgcgc
     2161 cagctgcgcc gctactacgg catctacacc ctcggcttcg cgcttttcgt cgtgctgctc
     2221 gcgatcggcg agagcctggg cctgtcgcag cagctcatcg gccacgtctt cctgttcgtg
     2281 acgatcgcga tctacgccac catcggcgtg ctctcgcgca cctccgacgt caccgagtac
     2341 tacgtcgccg gacgccgcgt gccggcgctg ttcaacggta tggcgaccgc ggccgactgg
     2401 atgagcgccg cgtccttcat cggtctggcc ggtaccttgt acttcagcgg cttcgagggc
     2461 ctggccttcg tgaccggatg gaccggcggc ttcgtgctgg tggccttgct gctcgcgccc
     2521 tacctgcgca agttcggcca atacaccatc cccgacttcc tcggcgcgcg ctaccagggc
     2581 aacgtcgcgc gcctggtcgg actggcggcg gcggtgctgg cgagcttcgt ctatctggtc
     2641 gcgcagatct acggcgtggg cctggtgacc agccgcttcg tcagcgtcga gttcgagatc
     2701 gggctcttca tcggcctggc cggcatcctg gtgtgctcct tcctcggcgg catgcgcgcc
     2761 gtgacctgga cccaggtggc gcagtacgtg atcctgatca tcgcctacct ggttccggtc
     2821 gtgatcctgt cctacaagct caccgggatt ccgatcccgc aggcggttta cggcaccgtg
     2881 ctgcagcaga tcagcgcgcg cgaggacgag ctcctcgagg ctcccaccga gcgcgcggtg
     2941 cgcgatctct acagcgcgcg cgcgcgcgac tacgccgaca agatcgccgc gctgcccgcc
     3001 tcgctcgagg acgagcgccg gcgcatgatc gacgagctca accgcatgcg cctggacagc
     3061 gcgccggcgc gcgacatcgc actgaccgag cgcgcgttgc gcgacctgcc gcgcacgccg
     3121 gccgaggccc gcgaggcctg gcagcgcgcg cgcgccgagg cgctcgagcg cgcccgcccg
     3181 ccgccgcgtc acgccgaggc gcatccgggc gccaccgcga gcgagtccgc caccgcgcgc
     3241 aacaacttcc tcgccctgat gttcgtgctg atggtcggca ccgcggcgct gccgcacatc
     3301 ctgatgcgct actacaccac gcccggcgtc atggaggcgc ggcgctcggt gctgtggtcg
     3361 ctgttcttca tcttcctgct ctacgtcacc gcgccggcct acgcggtgtt cgccaagtgg
     3421 gaggtctata acaatctggt cggctcgaac atcgcgatcc tgcccgactg ggtcgcgtcg
     3481 tggggcaggg tggggctggt gcgcatcgag gacctcaacg gcgacggcat cctgcaactc
     3541 gccgagctgc gcctgaacac cgacgtgatc gtgctcgcca ccccggagat cgccggcctg
     3601 ccctacgtgg tatcgggcct ggtcgcggcc ggggggctgg ccgccgcgct gtcgaccgcc
     3661 gacggccttt tgctgacgat ctcgaacgcg ctgtcgcacg atctctacta caaggtcatc
     3721 aaccccaagg cgagcgcgca ccggcgcctg gtgatctcca agtcgcagct gctggtggtg
     3781 gcggtggtgg cggcctgggt ggcctcgatg cggccggaca acatcctctt catggtcggc
     3841 ctggccttct cgatcggtgg ctcggccttc ttcccggcgc tcgtgctggg catcttctgg
     3901 aagcgcgcca accgcccggg ggcggtcgcc ggcatgctgg ccgggctggc ggtcacggtg
     3961 ttctacgtgg tgcgtaccca tcccttcttc ggcggcgcga tggcgaacgc ctggttcgaa
     4021 atcaacccga tctcggccgg cgtgttcggc gtgccgctcg gctttgcgac catcgtcgcg
     4081 gtcagcctgc tgaccaagcc accgccgcag gagatccagg acctggtcga ctacgtacgt
     4141 taccccgaca tcccgggaca aagccggccg catctttgag cgcgcacttg aagcgcgccg
     4201 gatgcgcccc acatagggtt cagttcaacg aatcccgagg atgacctcca tgcgtttcga
     4261 caagctcacc accaagttcc agcaggccct cgccgatgcg cagagcatcg ccgtgggcaa
     4321 cgaccagcag ttcatcgagc cgctgcacgt gctgcaggcc ctgctcgagc aggacgacgg
     4381 cggcagtgtg tcgctgctgc agcgcgccgg ggtcaatgtc ccgccgctca agaatgcggt
     4441 cgccaaggcc gtcggccgcc tgcccaaggt ggaagggcac ggcggcgagg tcagcatcgg
     4501 gcgcgacctc aacaacctgc tcaacgtcac cgaccgtgaa gcgcagaaac gcggcgacca
     4561 gttcatcgcc agcgagatgt tcctgctcgc gctcgcggac gacaagggcg aggccggccg
     4621 cctgctcaag gagaacggcc tggcgcgcaa ggcgctcgag gccgcgatcg aggcggtgcg
     4681 cggcggcgcc aacgtgggca gccaggacgc cgaaggccag cgcgaatcgc tgtcgaagta
     4741 ctgtctcgac ctcaccgagc gcgcacgcag cggcaagctc gacccggtga tcggtcgtga
     4801 cgacgagatc cgccgcgcca tccagatcct gcagcgccgc accaagaaca acccggtgct
     4861 gatcggcgag ccgggcgtgg gcaagaccgc gatcg
//
LOCUS       DAOQBS010000176         4857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_18314_length_4857_cov_11.538526, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000176 DAOQBS010000000
VERSION     DAOQBS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18314_length_4857_cov_11.538526"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PKN25_14110"
     CDS             complement(<1..990)
                     /locus_tag="PKN25_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNV92081.1"
                     /translation="MTEIRNVEQELSRFRTRLVVAALFVLFAFGLLIARLVHLQVYKH
                     DELATQAENNRIAVLPIVPIRGLIVDRNGIVLANNYSAYTLEITPSKVDDLDALIDRL
                     AEIVDIQARDRKRFKRLLQDSKSFESLPIRTKLSDEEVARFTAQRFRFEGVDIKARLF
                     RNYPLGEVGAHLLGYIGRINTAEKEKIDDSEDAANYAGTEYIGKLGVEQSYESDLHGT
                     TGFEEVETSAGGRAVRRLKSHPATPGNTVVLSVDIKLQAMVEQMFGDRRGAMVVLDPR
                     SGEVLAFVSKPTFDPNLFVDGIDVDSWRELNESIDKPLLNRALRGTYPPGSTFK"
     gene            complement(993..1514)
                     /gene="mreD"
                     /locus_tag="PKN25_14115"
     CDS             complement(993..1514)
                     /gene="mreD"
                     /locus_tag="PKN25_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNV92082.1"
                     /translation="MIMPRGADQLLLPANPLFVWLSLLVAFAINLVPLGRAPAMPDLL
                     ALVLVFWNVHQSRRVGIGLAFLFGVMMDVHDGAVLGQHALAYTLLSFFAITIHRRLLW
                     FTVPAQAVQILPLFVAAHVVALVVRMFAGGMFPGWNLLLAPLIEALLWPVATWLLLAP
                     QRRPPDPDENRPL"
     gene            complement(1570..2526)
                     /gene="mreC"
                     /locus_tag="PKN25_14120"
     CDS             complement(1570..2526)
                     /gene="mreC"
                     /locus_tag="PKN25_14120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNV92083.1"
                     /translation="MALGTLDRSPPPFFRQGMSALTKLTFFSALAVFLMVADTRFKLT
                     QPLRAVVATALHPVQRTLLVPVQAAASARDHLVGLQQALAERDAAQRELAAQAERSAR
                     VETLLAENARLRALLDLRPGLQVRSTAAQVLYEASDPYSRKVVIDRGATQGIALGAPV
                     VNEHGVLGQVTRLYPLSAEVTLLTDKDAAIPVLNTRTQARGAAFGSAGTLELRFMAGN
                     ADVQVGDLMTTSGVDGVYPSGLPVAKVTSVERKGESGFARILLTLVAPPDGVRQVLVL
                     EPIGLQLPPRPEPEPAAEVRPAAGRKAAAAAKAASAASGAAR"
     gene            complement(2592..3635)
                     /locus_tag="PKN25_14125"
     CDS             complement(2592..3635)
                     /locus_tag="PKN25_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNV92084.1"
                     /translation="MFGSFRRYFSTDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRHE
                     GGPNGKKTIQAVGAEAKAMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIKMVHPR
                     SVLRPSPRIIICVPCGSTQVERRAIRESALGAGASEVYLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYKGSVRVGGDKFDEAIINYIRRNYGMLIGEPTAEQ
                     IKKSIGSAFPGSEVKEIEVKGRNLSEGVPRSFTISSNEILEALTDPLNQIVSSVKNAL
                     EQTPPELGADIAERGMMLTGGGALLRDLDRLLAEETGLPVLVAEDPLTCVVRGCGMAL
                     ERMERLGSIFTSE"
     gene            3853..4152
                     /gene="gatC"
                     /locus_tag="PKN25_14130"
     CDS             3853..4152
                     /gene="gatC"
                     /locus_tag="PKN25_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNV92085.1"
                     /translation="MALTPQDVSRIAHLARLELSGAEQASLLTQLNGFFSIVEQMGAV
                     DTTGVEPLYTPLSAVQEVSLRLRDDAVTESDRRAFNQRSAPAVEDGLFLVPKVIE"
     gene            4149..>4857
                     /locus_tag="PKN25_14135"
     CDS             4149..>4857
                     /locus_tag="PKN25_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNV92086.1"
                     /translation="MKALHEMGVAELGRALAAREFSSVEATTALLDRLGRHASLGAVL
                     ASDRELVLKQAMAADARRAGGETGALLGVPIAHKDIFVSAELPTTAGSKMLEGYRSPF
                     DATVVARLAQAGTVTLAKLNCDEFAMGSSNENSAYTPVRNPWDTGRVPGGSSGGSAAA
                     VAARLVPAATGTDTGGSIRQPASFSGITGIKPTYGVCSRYGMVAFASSLDQAGPMARS
                     AEDCALLLSAMSGFDPRD"
BASE COUNT          705 a         1699 c         1733 g          720 t
ORIGIN      
        1 cttgaaggtg gaccccggcg ggtaggtgcc gcgcagcgcg cggttcagca gcggcttgtc
       61 gatcgactcg ttcagctcgc gccacgagtc gacgtcgatg ccgtcgacga acaggttcgg
      121 gtcgaaggtc ggcttgctga cgaaggccag cacctcgccg gagcgcgggt cgagcacgac
      181 catcgcgccg cgccggtcgc cgaacatctg ctcgaccatc gcctggagct tgatgtcgac
      241 cgacagcacg accgtgttgc ccggcgtggc cgggtggctc ttgagacgcc gcaccgcgcg
      301 cccgccggcg ctggtctcga cctcctcgaa gccggtggtg ccgtgcaggt cggactcgta
      361 ggactgctcg acgccgagct tgccgatgta ctcggtgccg gcgtagttgg cggcgtcctc
      421 cgagtcgtcg atcttctcct tctcggcggt gttgatgcgg ccgatgtagc ccagcaggtg
      481 ggcgccgacc tcgccgagcg ggtagttgcg gaacagccgc gccttgatgt ccacgccctc
      541 gaagcggaag cgctgcgcgg tgaagcgcgc gacctcctcg tcgctgagct tggtgcggat
      601 cggcagcgac tcgaagctct tgctgtcctg cagcaggcgc ttgaagcgct tgcggtcgcg
      661 cgcctggatg tcgacgatct ccgcgagccg gtcgatcagg gcgtcgaggt cgtcgacctt
      721 cgacggcgtg atctcgagcg tgtaggccga gtagttgttg gccagcacga tgccgttgcg
      781 gtcgacgatc aggccgcgga tcggcacgat cggcagcacc gcgatgcggt tgttctcggc
      841 ctgcgtggcg agttcgtcgt gcttgtagac ctgcaggtgc accaggcgcg cgatcagcag
      901 gccgaacgcg aacagcacga acagcgccgc cacgaccagc cgcgtgcgga agcgcgacag
      961 ctcctgctcg acgttgcgga tctcggtcat ggctacagcg ggcggttctc gtcggggtcg
     1021 ggcgggcggc gctgcggcgc cagcagcagc caggtcgcca ccggccacag cagcgcttcg
     1081 atcagcggcg cgagcagcag gttccagccc gggaacatgc cgcccgcgaa catccgcacg
     1141 accagcgcga ccacgtgcgc cgcgacgaac agcggcagga tctgcaccgc ctgtgccggc
     1201 accgtgaacc acagcaggcg ccggtggatg gtgatcgcga agaagctcag cagcgtgtag
     1261 gcgagcgcgt gctggccgag cacggcgccg tcgtgcacgt ccatcatcac cccgaacagg
     1321 aaggccaggc cgatgccgac gcggcgcgac tggtgcacgt tccagaacac cagcaccagc
     1381 gccagcaggt cgggcatggc cggcgcccgc ccgagcggca ccaggttgat cgcgaacgcg
     1441 accagcagcg acagccacac gaacagcgga ttggccggca gcagcagctg gtcggccccg
     1501 cgcggcatga tcatctcgag cctccgcaag acaggccgat gatgcgcgcc gcgcgcccgg
     1561 cacgcacgct catcgcgcgg cccccgacgc ggccgaggcc gccttggccg cggcggccgc
     1621 cttccggccc gccgccgggc ggacctcggc cgccggttcg ggctcggggc gcgggggcag
     1681 ctgcaggccg atgggttcga gcaccaggac ctggcgcacg ccgtcgggcg gcgcgaccag
     1741 cgtcagcagg atgcgggcga agccgctttc gcccttgcgc tcgaccgacg tcaccttggc
     1801 caccggcagc cccgaggggt agacgccgtc gacccccgag gtcgtcatca ggtcgccgac
     1861 ctgcacgtcg gcgttgcccg ccatgaagcg cagctcgagc gtgccggcgc tgccgaaggc
     1921 cgcgccgcgc gcctgggtgc gcgtgttcaa caccggaatg gccgcgtcct tgtcggtcag
     1981 cagcgtcacc tcggcgctca gcgggtacag gcgcgtgacc tggccgagca cgccgtgctc
     2041 gttgaccacc ggcgcgccca gcgcgatgcc ctgggtggcg ccacggtcga tgacgacctt
     2101 gcgcgaatac ggatcgctcg cctcgtacaa cacctgcgcc gcggtcgagc gcacctgcag
     2161 cccgggccgc aggtcgagca gcgcgcgcag ccgcgcgttc tcggcgagca gggtctcgac
     2221 acgcgccgag cgctcggcct gcgccgccag ctcgcgctgg gcggcatcgc gctcggccag
     2281 ggcctgctgc aggcccacga ggtggtcgcg ggccgacgcg gccgcctgca ccggcacgag
     2341 cagcgtgcgc tgcaccgggt gcagcgcggt ggcgaccacc gcgcgcagcg gctgggtcag
     2401 cttgaagcgc gtgtcggcca ccatcaggaa caccgccagc gccgagaaga aggtcagctt
     2461 ggtcagcgcc gacatgccct ggcggaagaa gggcggcggg gaacggtcga gcgtgcccag
     2521 ggccatcggt gtcggcggcg tcggacgtca gggggtgggc gccgcgggcg caccgcccgt
     2581 cgcggtcggt gtcactcgga ggtgaagatc gagcccaggc gctccatgcg ctcgagcgcc
     2641 atgccgcagc cgcgcaccac gcaggtcagc gggtcttcgg ccacgagcac cggcaggccg
     2701 gtttcctcgg cgagcaggcg gtcgaggtcg cgcagcagcg cgccgccgcc ggtgagcatc
     2761 atgccgcgct cggcgatgtc cgcgccgagc tcgggcggcg tctgctcgag cgcattcttg
     2821 acgctggaga cgatctggtt gagcgggtcg gtcagcgcct cgaggatctc gttcgacgag
     2881 atcgtgaagc tgcgcggcac gccttcgctg aggttgcggc ccttgacctc gatctccttg
     2941 acctcggagc cggggaaggc cgagccgatg ctcttcttga tctgctcggc ggtcggctcg
     3001 ccgatcagca tgccgtagtt gcggcggatg tagttgatga tcgcctcgtc gaacttgtcg
     3061 ccgccgacgc gcaccgagcc cttgtagacc atgccgccga ggctgatgac gccgacctcg
     3121 gtggtgccgc cgccgatgtc gacgaccatc gagccgctgg cctcgctcac cggcaggccg
     3181 gcgccgatcg cggcggccat cggctcctcg atcaggtaga cctcgctggc gccggcgccg
     3241 agcgcgctct cgcggatcgc gcggcgctcg acctgcgtcg agccgcaggg cacgcagatg
     3301 atgatgcgcg ggctcggccg cagcaccgag cgcggatgca ccatcttgat gaactgcttg
     3361 agcatctgct cggtgacggt gaagtcggcg atcacgccgt ccttcatcgg gcggatggcc
     3421 tcgatgttgc caggcacctt gccgagcatg gccttggcct cggcgccgac ggcctggatg
     3481 gtcttcttgc cgttggggcc gccttcatgg cggatggcga cgacggacgg ctcgtcgagc
     3541 acgatgccct tgccacgcac atagatcagc gtgttggcgg tgcccaggtc gatggccagg
     3601 tccgtcgaga agtagcgacg aaaggatccg aacatggtgt gggaagctct cgacctgcac
     3661 gccgccggac catcgggtcg ggcacggtga tgcggtcgat tggggttggc tgtttcgttc
     3721 ctttggcccc cgcaaaccaa ggctgggcgc gggccgcgag ggcttgtgga taaccgggga
     3781 taatacccca tcggcctgcc tccaccgggg tgcgggccgc gcgttttcac cctgtttccg
     3841 caccgacgtt tcatggccct gaccccccaa gacgtgagtc gaatcgccca ccttgcccgg
     3901 ctcgagctgt ccggcgccga gcaggcctcg ctgctgaccc agctcaacgg cttcttctcg
     3961 atcgtcgagc agatgggtgc ggtggacacc accggcgtcg agccgctgta cacgccgctg
     4021 tcggcggtgc aggaggtcag cctgcgcctg cgcgacgacg cggtgaccga atccgaccgg
     4081 cgcgccttca accagcgcag cgcgcctgcc gtggaagacg gcctgttcct cgtgcccaag
     4141 gtgatcgaat gaaagccttg cacgagatgg gcgtggccga actgggccgc gcgctggcag
     4201 ctcgcgagtt ctcgagcgtc gaggcgacca cggccttgct ggaccgcctg ggccgccatg
     4261 cctcgctggg cgccgtgctg gccagcgacc gcgaactcgt cctgaagcag gccatggcgg
     4321 cagacgcccg acgtgcgggc ggcgagaccg gcgcactgct gggcgtgccg atcgcgcaca
     4381 aggacatctt cgtgagcgcc gagctgccca ccaccgccgg ctcgaagatg ctcgagggct
     4441 accgcagccc gttcgacgcc acggtggtcg cccgtctcgc gcaggctggc acggtgacgc
     4501 tggccaagct gaactgcgac gagttcgcga tgggttcgtc caacgagaac tcggcgtaca
     4561 cccccgtgcg caacccctgg gacaccggcc gcgtgcccgg cggctcctcg ggcggctcgg
     4621 ccgccgcagt ggcagcgcgc ctcgtgcccg cggccaccgg caccgacacc ggcggctcga
     4681 tccgccagcc ggcgagcttt tccggcatca ccggcatcaa gccgacctac ggcgtgtgct
     4741 cgcgctacgg catggtcgcc ttcgcctcca gcctcgacca ggccgggccg atggcacgca
     4801 gcgccgagga ctgcgcgctg ctgctgtcgg cgatgagcgg cttcgacccg cgcgacg
//
LOCUS       DAOQBS010000177         4845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_18370_length_4845_cov_14.093528, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000177 DAOQBS010000000
VERSION     DAOQBS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18370_length_4845_cov_14.093528"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..918)
                     /locus_tag="PKN25_14140"
     CDS             complement(238..918)
                     /locus_tag="PKN25_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNV92087.1"
                     /translation="MSKEKDPQDFLAPTYYFDHEHPRVRAFIEESVGDAVDAVERAVK
                     LYYAVRDGIRYDPYRFYMRKEYFTASRTLIEGAAYCVPKANLLTAVARGAGIPARVGF
                     ADVRNHLSTERLRQLVGGDVYRWHGYVAMHLEGRWVKATPAFNLQMCERFDVHPLEFD
                     GRNDSLMHPYNARDERHMEYVKERGTFDDFPFEALVADMWEHHPGLVEAAEADLKGDF
                     EHEAMVDR"
     gene            complement(1048..1698)
                     /locus_tag="PKN25_14145"
     CDS             complement(1048..1698)
                     /locus_tag="PKN25_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV92088.1"
                     /translation="MKPPSLHALRILLADDHAVVRMGFRLLLEGAGAEVVGEADCGEA
                     AVQAFGQLHPDVLLMDVSMPGIGGLGALERVLAHHPAARVLMLSAHDDAQIPARALRA
                     GAGGYLSKRAHPDELLHAVATVARGQRYIDPAIAPALALAQFAGGGDPVAALTEKEFA
                     VFLQLARGRSVAEIAETHSLSPSTVGTHLYHIKQKLEVSNAAELALVAVRCGLIEV"
     gene            complement(1698..3104)
                     /locus_tag="PKN25_14150"
     CDS             complement(1698..3104)
                     /locus_tag="PKN25_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV92089.1"
                     /translation="MSGARTPSLQARLSLVLTVLIAVLMSAGAAVWMRETRQAIHEEV
                     EAATRVAQQWLVVVLAEQRDGDAEARARLLSRLAAVGRIRANRLEVFGVGGERLYVSP
                     ESPYKAGRDAPAWFAQHVAPELGARRLDAGALEVVLTPDTSRAVLDAWDHLVAALGWA
                     CALLLALWLATRVAIRRALAPLQQIHVALEHGARGRFDQRLPEYATRELALLARSYNR
                     LADRLDESRATNARLEADQSLARTIQARLEQERRAIARELHDELGQSITAVRSIAGAI
                     LQRSAEQPGIHGSAQAILAMTGHMQDGVHAILQRLRRDAPAARERIEDTLQAYCALWA
                     SHHEGIALRCQIEPLGEARDEPLDDGVGLAVLRVLQESLTNVARHARARSVEVRVGLR
                     REGGGRDGQAEIEVEVRDDGRGLGPQPTPGRHGLAGMRERLAELHGRLELHSPPTGGL
                     CVRARLPQALRQPLPVTQ"
     gene            complement(3101..3718)
                     /locus_tag="PKN25_14155"
     CDS             complement(3101..3718)
                     /locus_tag="PKN25_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HNV92090.1"
                     /translation="MALDHERLLALSAPAMEDLHRQLDSPTITVLLADSAGGVLRAIG
                     SRALPAAPAVPPGQLGIAVPILPPAGALLGFLDADAAPVEWLGHANALLHTAACLIEH
                     RLIETEPRGFLLLRFHRYASVLGTPLEALVLFDAEGAVLLANHTARALLAPCAFGAGG
                     AAACFATQWCGIVGYAALGLCEPFVLRDRRGGGFSAQASLRRQAT"
     gene            4151..>4845
                     /locus_tag="PKN25_14160"
     CDS             4151..>4845
                     /locus_tag="PKN25_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV92091.1"
                     /translation="MLQLFARLIGIVLLALAAGGAALAADRPLVRVGVLQFGTVGWEL
                     ETMQRHGLLEREGVDIRIVPLALKDAANVALQGGEVDVIVNDWLWVARMRSEGADFVF
                     VPFSQAVGGIHARPDAGIAGFADLRGQRLGVAGGALDKSWLLLRAYARRTVGEDAASF
                     LRPQFAAPPLLNELVTRGELPAAMNFWHYGARLAAAGLPEVLGMKRILATLGIGDEMP
                     LVGWVFGERWAGA"
BASE COUNT          606 a         1666 c         1895 g          678 t
ORIGIN      
        1 ttcctcgccc acgacggcga gcgcaagctc gtgcagttca cctaccagcc gcgcgcgccg
       61 ttcgccgtgc cgcggcagct gcgctggcag cgccgcgcgg tggtgtggcg caacacgccg
      121 ccggcggggg tgtgggagct gcaggcggtg cgctgagtcc ttcgcggcgg caagtcaccc
      181 gcgaagccgt gccgtgtccg ccacgccgcc gcccggcggt gctcgcgcgg tggccgctca
      241 gcggtcgacc atggcctcgt gctcgaagtc gcccttcagg tcggcctcgg ccgcttcgac
      301 cagacccggg tggtgctccc acatgtcggc gacgagcgcc tcgaagggga agtcgtcgaa
      361 ggtcccgcgc tccttcacgt actccatgtg gcgttcgtcg cgcgcgttgt aggggtgcat
      421 cagcgagtcg ttgcggccgt cgaattcgag cggatggacg tcgaagcgct cgcacatctg
      481 caggttgaac gccggcgtcg ccttcaccca gcggccttcc aggtgcatgg cgacgtagcc
      541 gtgccagcga tacacgtcgc caccgacgag ctggcgcagg cgctcggtgc tgaggtggtt
      601 gcgcacgtcg gcgaagccca cccgcgccgg gatgcccgcg ccgcgtgcga cggcggtgag
      661 caggttggcc ttgggcacgc agtaggcggc gccctcgatc agcgtgcggc tggcggtgaa
      721 gtactccttg cgcatgtaga agcggtaggg gtcgtagcgg atgccgtcgc gcaccgcgta
      781 gtacagcttc accgcgcgct cgaccgcatc gaccgcgtcg cccacgctct cctcgatgaa
      841 ggcgcgcacg cgcggatgct cgtggtcgaa gtaataagtc ggtgccagaa agtcctgcgg
      901 atccttctcc ttgctcattc ccggttctcc tcggtgtgcc tgcgttgtct cgggaatgta
      961 taaggggaag ttgacgttcg cgtcaatatt atttgcgccg cacgggtcgc cgcgatgtct
     1021 gctccgcggg cgccgacgcg cctgtcgcta gacttcgatg aggccgcagc gcaccgcgac
     1081 gagcgcgagc tcggcggcgt tcgacacttc gagcttctgc ttgatgtgat acaggtgggt
     1141 gcccaccgtg ctcgggctca ggctgtgggt ctcggcgatc tcggccacgc tgcgcccgcg
     1201 cgcgagctgc aggaagacgg cgaattcctt ctcggtgagt gccgcgaccg ggtcgccgcc
     1261 gccggcgaac tgcgcgagcg cgagggcggg cgcgatcgcc gggtcgatgt agcgctggcc
     1321 gcgcgcgacg gtggccaccg cgtgcagcag ctcgtccgga tgggcgcgct tggacaggta
     1381 gccgcccgcg ccggcacgca gcgcgcgcgc ggggatctgc gcgtcgtcgt gcgccgacag
     1441 catcagcacg cgcgccgcgg ggtggtgggc gagcacgcgc tcgagcgcgc cgagtccgcc
     1501 gatgcccggc atcgacacgt ccatcagcag cacatcgggg tgcagctggc cgaaggcctg
     1561 cacggccgcc tcgccgcaat cggcctcgcc gacgacctcg gcaccggcgc cttcgagcag
     1621 caggcggaag cccatgcgca ccaccgcatg atcgtcggcg agcaggatgc gcagggcgtg
     1681 gagggaaggg ggcttcatca ttgcgtgacc ggtaggggtt gccgcagagc ctgcgggaga
     1741 cgggcgcgca cgcacaggcc gccggtgggc gggctgtgca gttcgagccg gccgtggagt
     1801 tcggcgaggc gctcgcgcat gccggcgagg ccgtggcggc cgggggtggg ctgcgggccc
     1861 aggccgcggc cgtcgtcgcg gacctcgacc tcgatctccg cctgtccgtc gcgaccgccg
     1921 ccctcgcggc gcaggccgac gcgcacctcg accgagcgcg cgcgagcgtg gcgggcgacg
     1981 ttggtgaggc tctcctgcag cacgcgcagc acggcgaggc cgacgccgtc gtcgagcggc
     2041 tcgtcgcgag cctcgccgag gggctcgatc tggcaacgca gcgcgatgcc ttcgtgatgg
     2101 ctggcccaca gcgcgcagta ggcctgcagc gtgtcctcga tgcgctcgcg cgcggccggt
     2161 gcgtcgcggc gcaggcgctg caggatcgcg tgcacgccgt cctgcatgtg gccggtcatg
     2221 gcgaggatgg cctgcgcgct gccgtggatg ccgggctgtt cggcgctgcg ctgcaggatg
     2281 gcgccggcga tcgagcgcac cgcggtgatg ctctggccga gctcgtcgtg gagctcgcgc
     2341 gcgatcgcgc ggcgctcctg ctccaggcgg gcctggatgg tgcgcgcgag gctctggtcg
     2401 gcctcgaggc gggcgttggt ggcgcgcgac tcgtccaggc ggtcggcgag gcggttgtag
     2461 ctgcgcgcga ggagcgcgag ctcgcgggtg gcgtattccg gcaggcgctg gtcgaagcgg
     2521 ccgcgggcgc cgtgctcgag cgcgacgtgg atctgctgca gcggcgcgag cgcgcgccgg
     2581 atcgccacgc gggtggcgag ccagagcgcg agcagcagcg cgcacgccca gccgagcgcg
     2641 gcgacgagat ggtcccaggc gtcgagcacc gcgcgcgagg tgtcgggcgt cagcaccacc
     2701 tccagcgcgc cggcgtcgag ccggcgcgcg ccgagctcgg gcgcgacgtg ctgcgcgaac
     2761 caggccggcg cgtcgcgacc ggccttgtag ggcgactcgg gcgacacgta cagccgttct
     2821 ccgccgacgc cgaagacttc gagccggttg gcgcggatgc gcccgaccgc ggcgagccgg
     2881 ctcagcagcc gcgcgcgtgc ctcggcgtcg ccgtcgcgct gctccgccag caccacgacc
     2941 agccactgct gcgcgacccg ggtcgccgcc tcgacctctt cgtggatggc ctggcgcgtc
     3001 tcgcgcatcc acaccgccgc gcccgcgctc atcagcaccg cgatcagcac ggtgagcacc
     3061 aggctgaggc gggcctgcag gctgggcgtg cgcgcgccgc tcatgtcgcc tgccggcgca
     3121 ggctggcctg cgcggagaag ccgccgccgc gccggtcgcg cagcacgaag ggctcgcaca
     3181 ggccgagcgc ggcgtagccg acgatgccgc accactgggt ggcgaagcag gccgcggcgc
     3241 cgcccgcgcc gaaggcgcag ggcgcgagca gggcgcgcgc ggtgtggttg gcgagcagga
     3301 ccgcgccttc ggcgtcgaac agcacgagcg cttcgagcgg ggtgccgagc acgctcgcgt
     3361 agcggtggaa gcgcagcagc aggaagcccc gcggctcggt ctcgatgagg cggtgctcga
     3421 tgaggcaggc ggcggtgtgg agcagcgcgt tggcgtggcc cagccattcc accggggcgg
     3481 cgtcggcgtc gaggaagccg agcagggcgc cggccggggg caggatcggc accgcgatgc
     3541 cgagctggcc gggcggcacg gcgggggcgg ccgggagcgc gcgcgagccg atcgcgcgca
     3601 gcacgccgcc cgcgctgtcg gcgagcagca cggtgatcgt ggggctgtcg agctggcggt
     3661 ggagatcctc catcgccggc gccgacagcg cgagcaggcg ctcgtggtcg agggccacgt
     3721 cggcgccgcg gcgatcacgc gccgcgtcgt cgatggggtg cccgaccgtg cgcgtcgtcg
     3781 gcgtgccccg ggggggaggc ggggcggggg aagctgcggg agtcggtagg ctggacatcg
     3841 ctgtggtggt cctggagtcg ctgcggccga ggccgcccgt ggccacccat gcaggattcg
     3901 tgccgaggat gacgaggggg tgcggaccgc ggcgggacct gcttgcatgc agatgccggc
     3961 gcatagttgt tgcgtccctg agcaagcttg ctcccaaacg gaacagtcgc cgctgcgcca
     4021 gcgctgctgc gccgcgggca gtcgcgtgca ggggggcgcg atgtcgatcg ctggcacgga
     4081 acctgctggg gaatcggcac aaaaggcctg cgcccgttcc cgggccatgc aagacaacgg
     4141 aggagacccc atgctgcagc tgttcgcccg cctgatcggc atcgtcctgc tggcgctcgc
     4201 cgcggggggc gccgcgctcg ccgccgaccg cccgctggtc cgcgtcggcg tgctgcagtt
     4261 cggcacggtg ggctgggagc tcgagaccat gcagcgccac ggcctgctcg agcgcgaggg
     4321 ggtggacatc cgcatcgtcc cgctcgcgct caaggacgcc gccaacgtcg ccctccaggg
     4381 cggcgaggtc gatgtcatcg tcaatgactg gctgtgggtg gcgcgcatgc gctcggaggg
     4441 ggcggacttc gtcttcgtgc ccttctcgca ggcggtcggc ggcatccatg cgcgcccgga
     4501 cgcgggcatc gccggtttcg ccgacctgcg tggccagcgc ctcggcgtgg cgggcggcgc
     4561 gctcgacaag agctggctgc tgctgcgcgc gtatgcccgc cggaccgtgg gcgaggacgc
     4621 cgcgagcttc ctgcgcccgc agttcgccgc gccgccgctg ctcaacgagc tggtgacgcg
     4681 cggcgagctg ccggcggcga tgaacttctg gcattacggc gcccgcctcg ccgccgccgg
     4741 cctgcccgag gtgctgggca tgaagcggat cctcgccacg ctcggcatcg gcgacgagat
     4801 gccgctggtg ggctgggtgt tcggcgagcg ctgggcgggg gcgaa
//
LOCUS       DAOQBS010000178         4818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_18520_length_4818_cov_13.346210, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000178 DAOQBS010000000
VERSION     DAOQBS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18520_length_4818_cov_13.346210"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PKN25_14165"
     CDS             complement(<1..589)
                     /locus_tag="PKN25_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV92092.1"
                     /translation="MKLETIAVHGGYSPDPTTKAVAVPIYQTTSYAFDDTQHGADLFD
                     LKVQGNIYTRIMNPTTAVLEQRVAQLEGGIGALAVASGMSAITYAIQTIAEAGDNIVS
                     ASTLYGGTYNLFAHTFPQFGIEVRFADYRDPDSFAALIDARTKAIYCESVGNPLGNVT
                     DIGRLAEIAHKAGVPLIVDNTVPSPYLCRPFEHGAD"
     gene            complement(697..3732)
                     /locus_tag="PKN25_14170"
     CDS             complement(697..3732)
                     /locus_tag="PKN25_14170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV92093.1"
                     /translation="MSLSSFLYRSRATVAALVRSDGIAGRLVVAWLAFTVLLGGYWLQ
                     LDHSHTALVSQAESLTRLRALQTAHALALHTGALFRKLDYLSLHLGEHWLKEGPQGAR
                     DALVRAHLALPEDALVGIDVADAEGRLLYSTVGERLPLELERYGIADFGHFRVHLEGD
                     KGLFIGQPYRGRMTGAWIVPFSRALVEEGRLRGVIVLSVSVAHLTRALRELYPGTSDV
                     AALVLDDGHFLAHSNRTAGAIGRSVPSDRPYLLDRVALQGNFDATAPLDGVERYYAWH
                     RVAGFPVVAALGLSKADALAPVRGTIRDNRLRSGLGSLLLLVAALLISLLWRRHARQG
                     EELGRARRRLEKLVGHFPGMAYQFLLRADGSTSMPYCSPGVRELYGVGPEGLQDSAMS
                     LFERVHADDLDRLRDSILRSAANLAPWRCEFRVRGEGDRLRWLLGEANPERTPEGDTL
                     WHGYVHDITERQVAAQQLRESEARLRQAVDAVRDGLWSWDLARDRIALDERILEMLDR
                     PELGSEMCFDDFCALVHPADRQRQDAMLRAVPSATPDAPVAGDLRLRAASGRWVWIHA
                     RGSVVETDAQGGPRRLVGTFSDITARVAATQLRRALLDHSAAAIAMLDADLDILEANS
                     RAHEIFAPPSTPIGGLKLTDLALHEDQAIFIPQHYAALRAGGQVNIELPLRDNRGKKR
                     WFDVHGVMLDPEDPDSDTVWTLVDISDKYRTRMALATERLRLKTVLERFPGGVLMEDQ
                     DGLISSVNRGFCELLGLAAAPDELIGLTHAALCERLGTERMGWLHRPDADLTAEKRAT
                     VEVEGVKGRTLEIDWLPIEHDGRRLGRVWLLRDVTERKERERRLAELAATDPLTGLPN
                     RRSFLACLDAALDDARREPARGSALLMIDIDHFKHVNDTHGHPVGDEVLQHAARLIRG
                     GLRQHDRAGRLGGEEFAVLLDDVDADTALTLAERLRRSVEAAPAATAAGAVSLTISLG
                     LALVSGGDPARVLGDADAALYRAKRGGRNRVCVAVRTPG"
     gene            3887..4387
                     /locus_tag="PKN25_14175"
     CDS             3887..4387
                     /locus_tag="PKN25_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92094.1"
                     /translation="MYPIHDTDAQLLLATLIAAKRRPAALADIVAAAEFMGFPVTAPA
                     AWAVAFERFVTHGLLTTAGEGYVLTPLAVQLVEGLPRKAEADERVFLLRERLSAYKPA
                     TEHAAIAIGAEHFEAALREHARLAQQGGKNLLMPKPKRDDEDERRRPYGGRPGGGRFG
                     NARRRP"
     gene            complement(4435..>4818)
                     /locus_tag="PKN25_14180"
     CDS             complement(4435..>4818)
                     /locus_tag="PKN25_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNV92095.1"
                     /translation="LADGLVLARSAMHHSMNYRSVVVYGRFEAVTEPAHKLASLRAFI
                     DGLYPGRWDTLRPITEKELNATSVLRIALDEASAKVRDWGVKDDEEDLDWPVWAGVIP
                     LRTLAGEPVTEPDSVARSVPPTRFG"
BASE COUNT          715 a         1607 c         1784 g          712 t
ORIGIN      
        1 tgtcggcgcc gtgctcgaag gggcggcaca ggtagggcga gggcacggtg ttgtccacga
       61 tcagcggcac gccggccttg tgcgcgatct cggcgagacg gccgatgtcg gtgacgttgc
      121 cgagcgggtt gcccaccgat tcgcagtaga tcgccttggt acgcgcgtcg atgagcgcgg
      181 cgaagctgtc ggggtcgcgg tagtcggcga agcgcacctc gatgccgaac tgcgggaagg
      241 tatgcgcgaa caggttgtag gtgccaccgt acagcgtcga cgccgagacg atgttgtcgc
      301 cggcttcggc gatggtctgg atggcgtagg tgatcgccga catgcccgag gccacggcga
      361 gcgcgccgat gccgccttcg agctgggcca cgcgctgctc gagcaccgcg gtggtcgggt
      421 tcatgatgcg ggtgtagatg ttgccctgca ccttgaggtc gaagaggtcc gcgccgtgct
      481 gggtgtcgtc gaaggcgtac gaggtggtct ggtagatcgg caccgcgacg gccttggtgg
      541 tcggatcggg cgagtagccg ccgtgcacgg cgatggtctc gagcttcatg ggtcaggtct
      601 ccccttgggt gaaagtggcg gggagtatag ccagcgcggg cgggatggcg gaaccgccgc
      661 atgcgcgtgg tgcttcgatg ccggcggcgc gctggtctat cccggggtgc gaactgcgac
      721 gcagacacgg ttgcggccgc cgcgcttggc acggtagagc gcggcatcgg cgtcaccgag
      781 gacgcgggcg ggatcccctc ccgacacgag ggcaagaccg aggctgatgg tgagcgacac
      841 cgcgccggcc gcggtcgccg cgggcgccgc ctccacgcta cggcgcaggc gttcggcgag
      901 ggtgagggcg gtgtcggcgt cgacgtcgtc gagcagcacg gcgaattcct cgccgccgag
      961 gcggcccgcc cggtcgtgct ggcgcaggcc gccgcggatc agccgtgccg cgtgctgcaa
     1021 tacctcgtcg ccgaccgggt gcccgtgggt gtcattgacg tgcttgaagt ggtcgatgtc
     1081 gatcatcaac agtgcgctgc cgcgcgcggg ctcgcgccgg gcgtcgtcga gcgcggcgtc
     1141 caggcacgcc aggaagctgc gccggttggg cagcccggtc agcgggtcgg tggcggcgag
     1201 ctccgccagc cggcgttcgc gctccttgcg ctcggtgacg tcgcgcagca gccacacgcg
     1261 gccgaggcgt cgcccgtcgt gttcgatcgg cagccagtcg atctccagcg tgcgcccctt
     1321 cacgccctcc acttcgaccg tggcgcgctt ctccgccgtg aggtcggcgt cgggccgatg
     1381 cagccacccc atgcgctccg tacccagccg ctcgcacagc gccgcgtgcg tgaggccgat
     1441 cagttcgtcc ggggcggccg cgagaccgag cagttcgcag aacccccggt tcaccgagct
     1501 gatcaggccg tcctggtctt ccatcaggac gccgcccggg aagcgctcga gcaccgtctt
     1561 caggcgcagg cgctcggtgg cgagcgccat ccgcgtgcgg tacttgtcgg agatgtcgac
     1621 cagggtccag acggtgtcgc tgtcggggtc ctccgggtcc agcatcacgc catgtacgtc
     1681 gaaccagcgt ttcttgccac gattgtcgcg caagggcagt tcgatgttga cctgtccgcc
     1741 ggcccgcagc gccgcgtagt gctgcgggat gaagatggcc tggtcctcgt gcagggcgag
     1801 gtcggtgagc ttgagtccgc cgatcggggt cgagggcggc gcgaagatct cgtgggcgcg
     1861 gctgttggct tcgaggatgt cgaggtcggc gtcgagcatg gcgatggcgg ccgcgctgtg
     1921 gtcgaggagg gcccgacgca actgcgtggc ggcgacccgt gcggtgatgt cggagaaggt
     1981 gcccacgaga cgccgcgggc caccctgcgc gtcggtctcc acgacgctgc cgcgggcgtg
     2041 gatccagacc cagcgcccgg acgcggcgcg caggcggagg tcgcccgcca cgggagcgtc
     2101 gggcgtcgcg ctcggcacgg cgcgcagcat cgcgtcctgg cgctggcgat cggccggatg
     2161 gacgagcgcg caaaagtcgt cgaagcacat ctccgagccc agttccggac gatcgagcat
     2221 ctcgaggatg cgctcgtcga gcgcgatgcg gtcgcgggcg agatcccacg accacaggcc
     2281 gtcgcggacg gcgtcgaccg cctggcgcag gcgcgcctcg ctttcgcgca actgctgcgc
     2341 agccacctgg cgctcggtga tgtcgtgcac atagccgtgc cacagcgtgt cgccttcggg
     2401 cgtgcgctcg gggttggctt cgccgagcag ccagcgcagc cggtcgccct cgccccgcac
     2461 gcggaattcg cagcgccagg gcgcgaggtt cgcggccgag cggaggatgc tgtcgcgcag
     2521 acggtccaga tcgtcggcgt ggacgcgctc gaacaggctc atggccgaat cctgcagccc
     2581 ctcgggcccc accccgtaga gttcgcgcac gcccgggctg cagtagggca tggaggtgct
     2641 gccgtccgca cgcagcagga actggtaggc catgcccgga aagtgaccga ccagcttctc
     2701 cagccgcctg cgggcgcggc cgagctcttc gccctggcgg gcgtggcggc gccagagcag
     2761 gctgatcagc aaggccgcta cgaggagcag cagcgaaccc aggccgctgc ggaggcggtt
     2821 gtcgcggatc gtgccgcgca ccggggcgag cgcgtccgcc ttgctcagcc cgagcgcggc
     2881 gacgacggga aagcccgcaa cgcggtgcca ggcgtaataa cgctcgacgc cgtccagtgg
     2941 cgcggtcgca tcgaagttgc cctgcagcgc cacgcgatcg agcagatagg ggcggtcgga
     3001 cggcaccgag cgcccgatgg ctcccgcggt ccggttggag tgggcgagga agtgaccatc
     3061 gtcgagcacg agcgccgcca cgtccgaggt ccccggatag agttccctca gtgcccgggt
     3121 gagatgggcg accgacaccg acaggacgat cacgccgcgc agtcgcccct cctccacgag
     3181 cgcacgggaa aatggcacga tccaggcgcc ggtcatgcgt ccgcggtagg gctggccgat
     3241 gaagaggccc ttgtcccctt ccagatggac gcggaaatgc ccgaagtccg cgatgccgta
     3301 gcgctcgagt tcgagcggca ggcgctcgcc gacggtggag tagaggagcc gcccctcggc
     3361 atcggcgacg tcgatgccga ccagggcgtc ttcgggaagc gcaaggtgcg cacgcacgag
     3421 ggcgtcgcgc gcgccctgcg gaccttcctt cagccaatgc tcgcccaggt gcagggaaag
     3481 atagtcgagc ttgcggaaca gcgctccggt gtgcagcgcc aatgcgtgtg cggtctgcag
     3541 ggcacgcaac cgggtcaggc tctcggcctg ggagacgagc gcggtgtgcg agtggtcgag
     3601 ctgcaaccag tagccgccga gcagtaccgt gaacgccagc caggcaacca ccagcctgcc
     3661 cgcgatcccg tcgctgcgaa cgagggcggc gaccgtggcg cgcgaacgat acaggaagga
     3721 ggagagggac aagtcggttt caccgatctg taatggttcg agtctatcaa gttgcgagga
     3781 aatccgagcg gacgattgtt acgcggcacg acgacttccc ttgcgccgga tcgccgcgcc
     3841 tcgggcatca tggcgcgctt cacaacccga gaggcaggca aaacccatgt accccatcca
     3901 cgacaccgac gcgcagttgc tgctggcgac cctgatcgcc gcaaagaggc gacccgccgc
     3961 gctggccgat atcgtcgcgg ccgccgagtt catgggcttt ccggtcaccg cccccgcggc
     4021 gtgggcggtg gccttcgagc gcttcgtcac ccacggcctg ttgaccacgg cgggcgaggg
     4081 ctacgtgctg acgccgctgg ccgtgcagct ggtcgagggc ctgccgagga aggccgaggc
     4141 cgatgagcgc gtgttcctgc tgcgcgagcg cctgtcggcg tacaagccgg ccaccgagca
     4201 tgcggcaatc gcgatcggcg ccgagcactt cgaggcggcg ctgcgcgagc atgccaggct
     4261 ggcgcagcag ggcggcaaga acctgctgat gcccaagccc aagcgtgacg acgaggacga
     4321 gcgcaggcgt ccgtacggcg gccggcccgg cggcggccgc ttcggcaatg cgcggcggcg
     4381 tccctgatgg cgtagtggcg gtccgcgcga ggcgctcgcc gcccggttct tgccttaacc
     4441 gaagcgggtc ggcggtaccg atcgcgccac gctgtcgggc tcggtcaccg gctcgccagc
     4501 cagcgtgcgc agcgggatca cgcccgccca caccggccag tccaggtctt cctcgtcgtc
     4561 cttcacgccc cagtcgcgca ccttggccga cgcctcgtcg agcgcaatcc gcagcacgct
     4621 ggtggcgttc agctccttct ccgtgatcgg gcgcagcgtg tcccagcgtc cggggtagag
     4681 gccgtcgatg aaggcgcgca ggctggcgag cttgtgtgcg ggctcggtca ccgcctcgaa
     4741 gcggccgtag accaccaccg agcggtagtt catcgagtgg tgcatcgccg agcgcgccag
     4801 caccaggccg tcggcgag
//
LOCUS       DAOQBS010000179         4796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_18628_length_4796_cov_14.169163, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000179 DAOQBS010000000
VERSION     DAOQBS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18628_length_4796_cov_14.169163"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKN25_14185"
     CDS             <1..200
                     /locus_tag="PKN25_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNV92096.1"
                     /translation="AFGLDRIVTMMTGAESIRDVIAFPKTQRAQCLLTDAPSGVDEKQ
                     LRELHIRLRQKVETQVEVERG"
     gene            346..1995
                     /locus_tag="PKN25_14190"
     CDS             346..1995
                     /locus_tag="PKN25_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV92097.1"
                     /translation="MLLPHGLQVLVVESQSSMRAQLRNMLASIGIDSPQFAVSASMAM
                     RRLRAQPFDLILSEYDLGEGQDGQHLLEDLRQHAIIPPDTVFVMITGERNYERVISAC
                     ELVPNDYILKPLTAESLRVRLLRAFEKRDAFLPVWQLMKLGDPVGALAACREARDRHP
                     QYLVDFMRLQAELHAQIGQIEEAAALYREILAARPIPWASLGLARMLVVREAWVEAEH
                     LLADLVARHPRFIEAHELLARVQEETGRPAEACGTLQRAADLSPYRLPRLRRLGELAL
                     QAGELDRAEHSLAEAVRRGSYSEFRDPEDHVRLVQAQLARERPDEALATLADLERNLV
                     RHPKGELCLAVGNALFEGHRNDAEGLRSAMVRALASPERIRELSPGLRRDLLELCYDR
                     KLESEGRQLLADLMRTHGDDRSLASTRAALKARGFENLSAEVEEQVQNEVRTLIGAGA
                     EKARSGDYDGAVAEMMNAARKMPGNPHVLFNAALALLRHIEHRGWNEAFAAQARSLIE
                     RTQRMAPANPRLAAITEFRHELMKRYGIRPERAPVTRTRRA"
     gene            2002..2406
                     /locus_tag="PKN25_14195"
     CDS             2002..2406
                     /locus_tag="PKN25_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HNV92098.1"
                     /translation="MLRTVLLRLLLLLVLGAGLPACSPGAAPDAPQAAQGLLQPSHAG
                     LPAEAIETLALIQRGGPFPYRKDGTTFQNRERLLPARPRGYYREYTVPTPGARDRGAR
                     RIVSGGDPPEVFYYTADHYRSFREIAPLRSLP"
     gene            2418..2783
                     /locus_tag="PKN25_14200"
     CDS             2418..2783
                     /locus_tag="PKN25_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNV92099.1"
                     /translation="MSHTSHAPAAQPTPPLRIDLAGCTDKAGLLARIATALRFPDWFG
                     HNWDALSDCLCDLSWLPAAAYRLEFLHADALRATAPETFATLCEILDEAGDFWRAEGV
                     GFGHTFIPALPEAPSGAPR"
     gene            complement(2740..2943)
                     /locus_tag="PKN25_14205"
     CDS             complement(2740..2943)
                     /locus_tag="PKN25_14205"
                     /inference="COORDINATES: protein motif:HMM:NF025733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HNV92100.1"
                     /translation="MNETTNVCPRCGAAFTCGMRAGEAECWCAALPAAFAVPAEGAGE
                     CYCPRCLAELIEARRKVPREARG"
     gene            3104..3541
                     /locus_tag="PKN25_14210"
     CDS             3104..3541
                     /locus_tag="PKN25_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNV92101.1"
                     /translation="MSNPLTRRTDPLDDFFRGFFVRPVDFGGQQLATPGTDAPQMRVD
                     VKETAQGYEVHAELPGMKKEDIHVHIDGPVVSISAERKQEKEIKDGEKVLRTERYFGK
                     ISRSFQLGQEIDEAAASAKFNDGVLELNLPKKAEVQAKRLNIE"
     gene            complement(3822..4724)
                     /gene="argB"
                     /locus_tag="PKN25_14215"
     CDS             complement(3822..4724)
                     /gene="argB"
                     /locus_tag="PKN25_14215"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNV92102.1"
                     /translation="MTQPVHQQDVTPARKAEILAEALPYIKRFFDKTIVIKYGGNAMT
                     DPHLKDCFARDVVLLKLVGLNPVVVHGGGPQIENLLTRMGKKGEFIQGMRVTDAETME
                     VVEMVLGGQVNKEIVNLINKHGGKAVGLTGKDAGFIRAKKLLMQKHGAPEGDLIDVGQ
                     VGEITGIDPSLISFLDQGDFIPVIAPIGVGEEGETYNINADVVAGKLAEILKAEKLVL
                     LTNTPGVLDKAGNLLTGLTPRQIDDLVADGTLSGGMLPKIGSALDAARNGVKSVHIID
                     GRVEHCLLLEILTDHGVGTMIKSK"
BASE COUNT          749 a         1811 c         1534 g          702 t
ORIGIN      
        1 tggccttcgg cctcgaccgc atcgtcacca tgatgaccgg cgccgaatcc atccgcgacg
       61 tcatcgcctt ccccaagacc cagcgcgccc agtgcctgct caccgacgcg ccgagcggcg
      121 tggacgagaa gcagctgcgc gagttgcaca tccgcctgcg ccagaaggtc gagacccagg
      181 tcgaagtcga gcgcggctga gtccggcggc acaccggccg gagatcccga agcccctgcc
      241 ggatcgccgc gaaggcggcc gcaggggctt cagcctgccg gcgccatgcc gtaatcttca
      301 cggatggccc gcgcgccgcg cggccccccg atcccggacg ccccgatgct cctcccccac
      361 ggcctgcagg tcctcgtcgt cgaatcccag tcgagcatgc gcgcgcagct gcgcaacatg
      421 ctcgcctcga tcggcatcga ctcgccgcag ttcgcggtct cggcgagcat ggcgatgcgc
      481 cgcctgcgcg cgcagccctt cgacctcatc ctgtccgaat acgacctcgg cgagggccag
      541 gacggccagc acctgctcga ggacctgcgc cagcacgcca tcatcccgcc cgacacggtg
      601 ttcgtgatga tcaccggcga gcgcaactac gagcgcgtga tcagcgcctg cgagctcgtt
      661 cccaacgact acatcctcaa gccgctcacc gccgagagcc tgcgcgtgcg cctgctgcgc
      721 gccttcgaga agcgcgatgc cttcctgccg gtgtggcagc tgatgaagct cggcgacccc
      781 gtcggcgcgc tcgccgcctg tcgcgaggcg cgcgaccgcc acccgcagta cctggtcgat
      841 ttcatgcgcc tgcaggcgga gctgcacgcc cagatcggcc agatcgagga ggccgcggcg
      901 ctgtaccgcg agatcctcgc cgcgcgtccc atcccctggg cctcgctcgg gctggcacgc
      961 atgctggtgg tgcgcgaggc ctgggtcgag gccgagcacc tgctcgccga cctcgtcgcc
     1021 cgccacccgc gcttcatcga ggcgcacgag ctgctcgcgc gcgtgcagga ggagaccggc
     1081 cgccccgccg aggcctgcgg aaccctgcag cgcgcggccg acctctcgcc ctaccgcctc
     1141 ccgcgcctgc gccggctcgg cgagctcgcc ctgcaggccg gcgagctcga ccgtgccgag
     1201 cacagtctcg ccgaggcggt gcgccgcggc agctactccg aattccgcga ccccgaggat
     1261 cacgtccgcc tggtgcaggc ccagctcgcc cgtgaacgcc cggacgaagc cttggccacg
     1321 cttgccgacc tcgagcgcaa cctcgtccgc caccccaagg gcgaactctg cctcgccgtc
     1381 ggcaacgccc tgttcgaggg ccaccgcaac gacgccgaag gcctgcgcag cgccatggtg
     1441 cgcgcgctcg cgagccccga gcgcatccgc gagctctccc ccgggctgcg ccgcgacctg
     1501 ctcgagctgt gctacgaccg caagctcgag agcgagggcc gccagctcct cgccgacctg
     1561 atgcgcaccc acggcgacga ccgcagcctg gcgagcacgc gcgccgcgct caaggcacgc
     1621 ggcttcgaga acctctccgc cgaggtcgag gagcaggtgc agaacgaggt caggaccttg
     1681 atcggcgccg gcgccgaaaa agcgcgcagc ggcgactacg acggcgcggt cgccgagatg
     1741 atgaacgcgg cgcgcaagat gccgggcaac ccgcacgtgc tgttcaatgc cgcgctcgcc
     1801 ttgctgcgcc acatcgagca ccgcggctgg aacgaggcct tcgccgcgca ggcgcgcagc
     1861 ctgatcgagc gcacccagcg catggcgccc gccaacccgc gcctggcggc gattaccgaa
     1921 ttccgccacg agctcatgaa gcgctacggt atccgccccg agcgcgcccc cgtcacccgc
     1981 acccgccgcg cctgaacccc gatgctgcgt accgtcctgc tccgcctgct gctcctcctc
     2041 gtactcggcg cgggcctgcc cgcctgctcg cccggcgccg cgcccgatgc cccgcaagct
     2101 gcacagggcc tgctgcagcc ctcgcacgcc gggctgccgg ccgaggcgat cgagacactg
     2161 gcgctcatcc agcgcggcgg ccccttcccc taccgcaagg acggcaccac cttccagaac
     2221 cgcgaacgcc tgctaccggc gcggccgcgc ggctattacc gcgaatacac cgtgcccacg
     2281 cccggcgcgc gcgatcgcgg cgcgcgccgc atcgtcagcg gcggcgatcc acccgaggtg
     2341 ttctactaca ctgccgacca ctaccgcagc ttccgcgaga tcgcaccgct gcgttcgctg
     2401 ccctgagccg tccgtccatg agccacacct cgcacgcccc cgccgcgcag ccgactcccc
     2461 cgctgcgcat cgacctcgcc ggctgcacgg acaaggccgg actgctcgcg cgcatcgcga
     2521 ccgcgctgcg cttcccggac tggttcggcc acaactggga cgcgctctcg gattgcctgt
     2581 gcgatctctc ctggctgccc gccgcggcct accggctcga attcctgcat gccgacgcgc
     2641 tgcgcgcgac cgcgcccgag accttcgcca cgctgtgcga gatcctcgac gaagccggcg
     2701 acttctggcg cgccgagggc gtgggcttcg gccacacctt catccccgcg cttcccgagg
     2761 caccttccgg cgcgcctcga tgagctcggc gaggcagcgc gggcaatagc actcccccgc
     2821 accttcggcc ggcaccgcaa acgcggccgg cagcgccgca caccagcact ccgcctcgcc
     2881 tgcgcgcatg ccgcaggtga aggccgcgcc gcagcgcgga cagacattgg tggtttcgtt
     2941 catcggcgtc tcgcctccct ggttcgttcg aactccgccg caaggttagc gcagccggac
     3001 gggcagaggg tacgcgcgcg ccccttgaaa acgcttcctc cgcgcctata ttggtcttga
     3061 agccggatcc cccggcaaga acccggaccc aggaggacac gacatgagca accctctcac
     3121 ccgtcgcacc gacccgttgg acgacttctt ccgcggtttc ttcgtgcgcc ccgtcgactt
     3181 cggcggccag cagctcgcca ccccgggcac cgacgcgccg cagatgcggg tcgacgtcaa
     3241 ggagaccgcg caaggctacg aagtgcatgc cgaactgccc ggcatgaaga aggaggacat
     3301 ccacgtgcat atcgacggcc cggtggtgtc gatcagtgcc gagcgcaagc aggagaagga
     3361 gatcaaggac ggcgagaagg tgctgcgcac cgaacgctac ttcggcaaga tctcgcgcag
     3421 cttccagctc ggccaggaaa tcgacgaggc tgcggcaagc gcgaagttca acgatggcgt
     3481 gctcgagctg aacctgccca agaaggccga ggtccaggcc aagcggctca acatcgagta
     3541 gacgcctgcc cggcagctcg ggacaaacgg gattcaggag tggcgcgatt cgcgagcggg
     3601 tcttcagcca gagaccgagt tcggcaagtg cggcgcggca gccatcgcga ctcgcgtcgc
     3661 tcctacagcc cccccgaagc ctcgccggtt cgcgtaggag cgacgcaagt cgcgatcccc
     3721 ctgcagcgcc cccgaagcct cgccggttgt cgtaggagcg acgcaagtcg cgagccgcgt
     3781 cgcacgccat cctcgacggc ggcgcggcaa acgcgcgggt tctacttgct cttgatcatc
     3841 gtgccgacgc cgtggtcggt gaggatctcg agcaacaggc agtgttccac gcggccatcg
     3901 atgatgtgca ccgacttcac gccgttgcgc gccgcgtcga gcgccgagcc gatcttgggc
     3961 aacatgcccc ccgacagggt gccgtcggcg accaggtcgt cgatctggcg cggcgtcagg
     4021 ccggtcagca ggttgcccgc cttgtccagc acgcccgggg tgttggtgag cagcaccagc
     4081 ttctcggcct tgaggatctc ggcgagcttg ccggcgacca cgtcggcgtt gatgttgtag
     4141 gtctcgccct cctcgcccac gccgatcggc gcgatcacgg ggatgaagtc gccttggtcg
     4201 aggaaggaga tcaggctggg atcgataccg gtgatctcgc ccacctggcc gacgtcgatc
     4261 agatcgccct ccggcgcgcc gtgcttctgc atcagcagct tcttggcgcg gatgaagccg
     4321 gcgtccttgc cggtgaggcc cacggccttg ccgccgtgct tgttgatcag gttgacgatc
     4381 tccttgttca cctggccccc gagcaccatc tcgaccacct ccatggtctc ggcgtcggtc
     4441 acgcgcatgc cctggatgaa ctcgcccttc ttgcccatgc gggtgagcag attctcgatc
     4501 tgcgggccgc cgccgtgcac caccaccggg ttgaggccga ccagcttgag cagcaccacg
     4561 tcgcgtgcga agcaatcctt gaggtgcgga tcggtcatcg cgttgccgcc gtacttgatg
     4621 acgatggtct tgtcgaagaa gcgtttgatg tagggcagcg cctcggcgag gatctcggcc
     4681 ttgcgggcgg gggtgacgtc ttgctggtga acgggctggg tcatcgggag actccttgaa
     4741 cggacacggg gattctagtg gccagcccgc ggaaccggaa gtgcaccgca gcaaag
//
LOCUS       DAOQBS010000180         4763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_18819_length_4763_cov_16.439252, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000180 DAOQBS010000000
VERSION     DAOQBS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18819_length_4763_cov_16.439252"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1850)
                     /gene="glmS"
                     /locus_tag="PKN25_14220"
     CDS             complement(24..1850)
                     /gene="glmS"
                     /locus_tag="PKN25_14220"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNV92103.1"
                     /translation="MCGIVTAIATNNVVPVLLEGLRKLEYRGYDSAGLAVLAGELTRL
                     RSCGRVAELAALADARQLSAHIGIAHTRWATHGVPSERNAHPHISGGLAVVHNGIIEN
                     FESIKADLQGKGYVFESETDTEAIAHLVHYKLQANTDLFEAVRQATNELVGAYAIGVV
                     AEADPFRVIVARRGSPLLLGIGEDGMYAASDTAALLQVTRKIVYLEDGDLAELRRDSY
                     IVIRSDGSPVEREVHISSLSADAVELGKFRHYMQKEIFEQPQALAQTLELIAGGAGIT
                     PQHFGARAGEVFDGVRRVLVLACGTSYHAGLVARYWIESIAKLPVTVEIASEYRYRES
                     VPDPDTLVVVISQSGETADTLAALKHAKALGMQRTLAICNVPESAIVRETALRFITRA
                     GPEIGVASTKAFTTQLAAFALLTLALAKINGRLSAEDEERRLAALRHLPVAVQKVLEL
                     EPEIEQWAQRFAGKEHALFLGRGSHWPIAMEGALKLKEISYIHAEAYAAGELKHGPLA
                     LVDKDMPVIAVSPADELLEKLKSNLQEVRARGGELYVFADAGSEIPESEGVHILHMPE
                     HYGMLSPVLHVIPLQLLSYHAALVKGTDVDKPRNLAKSVTVE"
     gene            complement(1922..3283)
                     /gene="glmU"
                     /locus_tag="PKN25_14225"
     CDS             complement(1922..3283)
                     /gene="glmU"
                     /locus_tag="PKN25_14225"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNV92104.1"
                     /translation="MQVVVLAAGQGKRMRSVLPKVLQPIAGKPMLAHVLDAARTLDAQ
                     RICVVYGHGGEVVRERLDAVDLAWARQEPQLGTGHAVQQALPHLDDEHVALVLYGDVP
                     LIGVPTLRRLVAAAGDARLALLTVEMDNPTGYGRILRDAAGKVVRIVEEKDASDEERK
                     VREVNTGILVAPVRHLRAWLGRIGNDNAQGEYYLTDIIGLAVADGVEVTTVQPDAFAE
                     TLGVNNKMQLAELERIHQGNIARRLMEEGVTLIDPARIDVRGTLTVGRDVEIDVNCVF
                     EGEVELGDGVRIGANCVVRDARIGAGTRLEPFSHVDSTTMGQACVIGPYARTRPGTVL
                     GTDVHLGNFVEIKNSVIADHSKANHLAYVGDADVGSKVNIGAGTITCNYDGANKHRTI
                     IEDEVFIGSDTQLVAPVRVGRGATLGAGTTLTKDAPAGQLTVSRSKQISLEHWKRPVK
                     QPR"
     gene            3560..>4763
                     /locus_tag="PKN25_14230"
     CDS             3560..>4763
                     /locus_tag="PKN25_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNV92105.1"
                     /translation="MKAPAAQQMIQNRTFDEIQVGDFAQLVRTLRPEDIHLFAAMSGD
                     VNPTHVDLEYARSSQFREVVGHSMWGSTLISTVLGTEFPGPGTVYVSQGLNFWRPITI
                     GDTLTITVTCKEKFEHNHHIIFDCLAVNQDGLKVIDGIAEVMAPTEKISRARVVLPEV
                     TVSDRELRYRHLLSVSAGLSPIPIAVAHPCDRESLLGPVQAARAGLVEPILVGPESRI
                     RAVAEEHEIDIKGLRIVDTEHSHASAEVAVALCRDGGAEALMKGSLHTDEMMSQVIKH
                     LRTGRRISHVFLADVPTYPHPLMITDAAIMIEPTLEDKVDIIQNAIELAHILGLPEPK
                     VAILSAVETVTSKIRSTIDAAALCKMADRGQIKGGLLDGPLAFDNAVSLVAAKTKGIK
                     SVVAGNADI"
BASE COUNT          726 a         1552 c         1715 g          770 t
ORIGIN      
        1 accggatatc aagtactacg tcttcactcg acagtgaccg acttcgcgag gttgcggggc
       61 ttatccacgt ccgtcccctt caccagcgcc gcgtggtacg acagcagctg cagcgggatc
      121 acgtgcagca ccggcgacag catgccgtag tgctcgggca tgtgcaggat gtgcacgcct
      181 tccgactcgg ggatctcgct gcccgcgtcg gcgaagacgt agagctcgcc gccgcgcgcg
      241 cggacttcct gcaggttgga cttgagcttt tcgagcagct cgtcggccgg ggacacggcg
      301 atcaccggca tgtccttgtc caccagggcg agcgggccgt gcttgagctc gccggcggcg
      361 taggcttcgg cgtggatgta ggagatctcc ttgagcttga gcgcgccttc catggcgatc
      421 ggccagtggc tgccgcggcc caggaagagc gcgtgctcct tgccggcgaa gcgctgcgcc
      481 cactgctcga tctcgggctc gagctcgagc accttctgca ccgcgaccgg caggtggcgc
      541 agcgcggcga ggcggcgctc ctcgtcttcg gcggacaggc ggccgttgat cttggcgagc
      601 gcgagcgtga gcagcgcgaa ggcggcgagc tgggtggtga aggccttggt ggaggcgacg
      661 ccgatctcgg ggccggcgcg ggtgatgaag cgcagcgcgg tctcgcgcac gatggcggat
      721 tcgggcacgt tgcagatcgc cagcgtgcgc tgcatgccga gcgccttggc gtgcttgagc
      781 gcggccaggg tgtcggcggt ctcgcccgac tgcgagatga ccacgaccag ggtgtcgggg
      841 tcggggacgg actcgcggta gcggtattcg ctggcgatct cgacggtgac cggcagcttg
      901 gcgatggatt cgatccagta gcgcgcgacc aggccggcgt ggtagctggt gccgcaggcg
      961 agcacgagca cgcggcgcac accgtcgaag acttcgcccg cgcgcgcgcc gaagtgctgc
     1021 ggggtgatgc cggcaccgcc ggcgatcagc tccagcgtct gcgccagggc ctgcggctgc
     1081 tcgaagattt ccttctgcat gtagtggcgg aacttgccga gttcgaccgc gtcggccgac
     1141 aggctggaga tgtgcacctc gcgctcgacc gggctgccgt cggagcggat cacgatgtag
     1201 ctgtcgcggc gcagctcggc gaggtcgccg tcttccagat agacgatctt gcgcgtgacc
     1261 tgcagcagcg cggcggtgtc ggaggcggcg tacatgccgt cctcgccgat gccgagcagc
     1321 agcggcgagc cgcggcgcgc gacgatgacg cggaaggggt cggcctcggc gaccacgccg
     1381 atggcgtagg cgccgacgag ctcgttggtg gcctggcgca cggcctcgaa gaggtcggtg
     1441 ttggcctgca gcttgtagtg gacgaggtgg gcgatggcct cggtgtcggt ctcggactcg
     1501 aagacgtagc ccttgccctg caggtcggcc ttgatggatt cgaagttctc gatgatgccg
     1561 ttatgcacca cggcgaggcc gcccgagatg tgcgggtggg cgttgcgctc ggagggcacg
     1621 ccgtgggtgg cccagcgcgt gtgcgcgatg ccgatgtggg cggagagctg gcgcgcatcg
     1681 gcgagcgcgg cgagctcggc cacgcggccg caggagcgca ggcgggtgag ctcgccggcg
     1741 agcacggcga ggccggccga gtcgtagccg cggtactcga gcttgcgcag gccctcgagc
     1801 aggaccggga cgacgttgtt ggttgcgatt gcggtgacga tgccacacat ggcggggctt
     1861 ccttgtcggt ctttggattc ggtgcggagg gcggggcggg cgaatcggct ggcggagggg
     1921 ctcagcgcgg ctgcttgacc gggcgcttcc agtgttcgag gctgatctgc ttgctgcgcg
     1981 acacggtgag ctggccggcg ggggcgtcct tggtgagcgt ggtgccggcg cccagggtgg
     2041 cgccgcggcc cacgcgcacc ggcgcgacga gctgggtgtc ggagccgatg aagacctcgt
     2101 cctcgatgat ggtgcggtgc ttgttggcgc cgtcgtagtt gcaggtgatg gtgccggcgc
     2161 cgatgttgac cttgctgccg acgtcggcgt cgcccacgta ggcgaggtgg ttggccttgg
     2221 agtggtcggc gatgacgctg ttcttgatct cgacgaagtt gcccaggtgc acgtcggtgc
     2281 cgagcacggt gccggggcgg gtgcgggcgt agggcccgat cacgcaggcc tggcccatgg
     2341 tggtgctgtc gacgtggctg aagggctcga gccgggtacc ggcgccgatg cgggcgtcgc
     2401 ggacgacgca gttggcgccg atgcgtacgc cgtcgccgag ctcgacctcg ccctcgaaga
     2461 cgcaattgac gtcgatctcg acgtcgcgcc cgaccgtgag cgtgccgcgc acgtcgatgc
     2521 gcgccgggtc gatcagggtg acgccctctt ccatcaggcg gcgggcgatg ttgccctggt
     2581 ggatgcgctc gagctcggcg agctgcatct tgttgttgac gcccagcgtc tcggcgaagg
     2641 cgtcgggctg cacggtcgtc acctcgacgc cgtcggccac ggccagcccg atgatgtcgg
     2701 tgaggtagta ctcgccctgg gcgttgtcgt tgccgatgcg gccgagccag gcgcgcaggt
     2761 ggcggaccgg ggcgaccagg atgccggtgt tgacctcgcg caccttgcgc tcctcgtcgg
     2821 aggcgtcctt ctcctcgacg atgcgcacca ccttgccggc ggcgtcgcgc aggatgcggc
     2881 cgtagccggt cgggttgtcc atctcgacgg tgagcagcgc caggcgcgcg tcgccggcgg
     2941 cggcgaccag gcggcgcagc gtgggcacgc cgatcagcgg cacgtcgccg tacagcacca
     3001 gggccacgtg ctcgtcgtcg aggtggggca gcgcctgctg caccgcatga ccggtgccga
     3061 gctgcggctc ctgccgcgcc caggccaggt cgacggcatc gaggcgctcg cgcaccactt
     3121 cacccccatg gccgtacacc acgcagatgc gctgggcgtc gagcgtgcgg gcggcatcga
     3181 gcacgtgggc cagcatcggc ttgccggcga tgggttggag gaccttgggc agcaccgagc
     3241 gcatgcgctt gccctggccg gcagcgagaa cgacgacttg catggttgat tccgtggcga
     3301 aatgaggaga ttcgacaggt ttcgggcgca caaccgaccc catccgcgcc cgcagatcgt
     3361 aaaaacgcgc ggagtctagc acgcgacccg cgcagccaat cgtgagcaat cgcatggtgc
     3421 gggcatgaaa aacatccgcg ccgcaaggaa acgtgcgtct gattgacgca ttgcacaatt
     3481 cgatcgtttt agcgcatgtg gcagtcacat acaaggtcta gaatcgcgtg ttgcaatgca
     3541 tccaagaacg agagttgcca tgaaagctcc tgccgcacaa cagatgatcc agaaccgcac
     3601 cttcgacgag atccaggtgg gcgacttcgc ccagctcgtc cgcacgctgc gccccgagga
     3661 catccacctg ttcgccgcca tgtcgggcga cgtcaacccg acccacgtcg atctcgaata
     3721 cgcacgctcc agccagttcc gcgaggtcgt cggccacagc atgtggggca gcacgctgat
     3781 ctccaccgtg ctcggcaccg agttccccgg ccccggcacg gtgtacgtgt cgcaggggct
     3841 gaacttctgg cgcccgatca cgatcggcga cacgctgacg atcacggtca cctgcaagga
     3901 aaagttcgag cacaaccacc acatcatctt cgactgcctg gcggtgaacc aggacggtct
     3961 caaggtgatc gacggcatcg ccgaggtgat ggcgccgacc gagaagatct cgcgcgcgcg
     4021 cgtcgtgctg cccgaggtca ccgtgtccga ccgcgagctg cgctaccgcc acctgctgtc
     4081 ggtgtcggcc ggcctgtcgc cgatcccgat cgcggtggcc cacccctgcg accgcgaatc
     4141 cctgctcggc ccggtccagg cggcacgcgc cggcctggtc gagccgatcc tggtcggccc
     4201 cgagtcgcgc atccgcgccg tggccgaaga acacgagatc gacatcaagg gcctgcgcat
     4261 cgtcgacacc gaacacagcc acgcctcggc cgaggtggcc gtcgccctgt gccgcgacgg
     4321 cggcgccgag gcgctgatga agggctcgct gcacaccgac gagatgatga gccaggtgat
     4381 caagcacctg cgcaccggcc gccgcatcag ccacgtcttc ctcgccgacg tgccgaccta
     4441 cccgcacccg ctgatgatca ccgacgcggc gatcatgatc gagcccaccc tcgaggataa
     4501 ggtcgacatc atccagaacg cgatcgagct cgcccacatc ctcggcctgc ccgaacccaa
     4561 ggtggcgatc ctgtcggcgg tcgagaccgt gacctcgaag atccgctcga cgatcgacgc
     4621 cgccgcgctg tgcaagatgg ccgaccgcgg ccagatcaag ggtggcctgc tcgacggccc
     4681 gctcgccttc gacaacgcgg tgtcgctggt cgccgccaag accaagggca tcaagtcggt
     4741 cgtggccggc aacgccgaca tcc
//
LOCUS       DAOQBS010000181         4675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_19353_length_4675_cov_14.474459, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000181 DAOQBS010000000
VERSION     DAOQBS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19353_length_4675_cov_14.474459"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..2447
                     /locus_tag="PKN25_14235"
     CDS             54..2447
                     /locus_tag="PKN25_14235"
                     /inference="COORDINATES: protein motif:HMM:NF017116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HNV92106.1"
                     /translation="MALCKNYDEVLSLLMAAGLEIEGPLQIGTSKMVRCYMDGDRKKK
                     RGAYRLFETPLSNGDSVILGTYGFARCAEYFFEKIPFPKGERKSVDAQQMAAIRERML
                     LEAKRAEAERLKRGDEAAAQASTWWGKLSDTGACAYLQRKGLPPGRLYGARVSSSGNL
                     VVPMQDGDGKVWGLQVIYGDPATKAKKGRDKDFTPPGLIKKGHWFQIGSPIAGGIVLL
                     CEGFATGASLHEATGLPVVVAFDAGSLLPVATAIRKRYRGVRVLVCADDDYLTEPNTG
                     VHAAQAAALAIDGAYVVPVFPVERSTDKAKKGPTDFNDLHVLPEGGLHAVRAQIDAAI
                     ASAGWVSSAATARGAAPQRGEGSKPGERPAALSTMALPEIVERFIHVDDSTGEFAFDL
                     WTQEMVKRSKIVAMLPARVRWDDVKDHPTWQSRATYIDEIGFDPTGRDSTIKLNKWKG
                     WPTKPRDGKCELLLDLLRYLCGNEANSDEVYRWMLCWLAYPLQHPGAKMQSAVVVHGP
                     QGTGKSRFFEAYAKIYAHHAVVLTQSALEDKFNSDWGQKLFGVADEVVARGEMHHVKN
                     FLKNLITSETIRINPKNLPAYTERNHINLVFLSNEKQPIVLENDDRRHLVIWTPGKLS
                     EVFYDEVAAEIEAGGIEALHHHLLHLDLGDFKPWTRPPMTKAKAQLIDTNRESVDRFL
                     LDWQSGDIEGMPFCPCGSADLYTAYLQWARREGVRFPRESNQFAGHVEKLPGWVKGHK
                     DRYADTYYTGKPVRQRFVIPAIEALPPEHAQRKDETQTQWLTRCYFAFRAALQGGQS"
     gene            2756..3370
                     /locus_tag="PKN25_14240"
     CDS             2756..3370
                     /locus_tag="PKN25_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92107.1"
                     /translation="MTLETKAAFARRLDWNRSTVTRAAQAGRLVLSGALVDVEASLAR
                     LKATEGGRADVAARHAAERGQGGDAHAPAAENTATAPHAPRSAENEAAPTSDTDGAGR
                     ARYKAITLQFENQQIKLGMAMARGLRFNRKDVAREAVALGAIVRSAVERVIDQTAPRL
                     AVMNAPDRRRELLGAELRAVRRALRAEFPRALRRLRSASQRGDA"
     gene            3367..3621
                     /locus_tag="PKN25_14245"
     CDS             3367..3621
                     /locus_tag="PKN25_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92108.1"
                     /translation="MTELFCHEGNVGEFNAALREHPDLRAFAAALHQAGMIDGLRGAR
                     LRPADAPRPENLQGVTPVLSDAAESRLADLWWQRKQEPKA"
     gene            3618..3857
                     /locus_tag="PKN25_14250"
     CDS             3618..3857
                     /locus_tag="PKN25_14250"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HNV92109.1"
                     /translation="MKPQIEHLPLDALVPYARNSRTHSPEQIAQVAASIREFGFTNPV
                     LIDGEGGIIAGHGRVMAARQLGLSEVPCIRLNRIG"
     gene            3864..>4675
                     /locus_tag="PKN25_14255"
     CDS             3864..>4675
                     /locus_tag="PKN25_14255"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HNV92110.1"
                     /translation="MASLADLQAERERLRAAQAKADFEKLLADTCALDDVRADAVAAR
                     AVLLQTLDALVGRWLSAIDDEHDETRVHYLMSDVAHDVLRELGESVQAATTRLPMLGE
                     RIARGAKPRDLLTVSQHADRYRVIRTGTNAPGPWRTALTPYLRDIMDDLSEHSPVRKV
                     VFIKSSGVGGTEAMYNWLGYVMHHLQNKDLLVVVPTLELRDRSFNPRLAKMIDESEAL
                     ANLVTTASRNKANRGDLLEYGARARIIKAGANSPDSLRSDHVPYVICDEVDA"
BASE COUNT          869 a         1742 c         1450 g          614 t
ORIGIN      
        1 ggtacgtgga ggcgcggttt ccccctaggc tgcgggctcg gggcataggc aacatggccc
       61 tctgcaagaa ctacgacgag gtgctgtcgc tgctgatggc ggccgggctc gagattgaag
      121 gcccgctgca gatcggcacc tcgaagatgg tccgctgcta catggacggc gaccgcaaga
      181 agaagcgcgg cgcgtaccgc ctcttcgaga cgcctctctc caacggcgac agcgtgatcc
      241 ttggcaccta cggcttcgca cgttgcgccg agtacttctt cgagaagatc ccgtttccca
      301 agggcgagcg caagtcggtc gatgcgcagc aaatggcagc gatccgcgag cgcatgctgc
      361 tcgaggcaaa acgcgccgaa gccgagcgcc tcaaacgtgg cgacgaagcc gcggcccagg
      421 cctcgacctg gtggggaaag ctgtccgaca ccggcgcctg cgcctacctg cagcgcaagg
      481 gcctgccgcc gggcaggctg tacggcgccc gcgtgtcgag ctccggcaac ctggtcgtgc
      541 cgatgcagga cggtgacggc aaggtttggg gcctgcaggt catctacggc gacccggcaa
      601 ccaaggccaa gaaggggcgc gacaaggact tcaccccgcc cggcctcatc aagaagggcc
      661 actggttcca gatcggcagc ccgatcgccg gcggcatcgt gctgctgtgc gaaggcttcg
      721 ccaccggcgc cagtctgcac gaggcgaccg gcctgccggt ggtggtggcc ttcgacgccg
      781 gctcgctgct gccggtggcc acggccatcc gcaagcgcta ccgcggcgtg cgcgtcctgg
      841 tgtgcgccga cgacgactac ctcaccgaac cgaacaccgg cgtgcatgcc gcccaggctg
      901 cagcgcttgc catagatggc gcttatgtcg tgccggtttt cccggtcgag cgcagtaccg
      961 acaaggccaa gaaaggcccg accgacttca acgacctgca cgtgctgccc gagggcggcc
     1021 tgcatgccgt gcgcgcgcag atcgacgccg ccatcgcatc ggcaggctgg gtttccagcg
     1081 ccgcgaccgc gcgcggggcg gccccacaaa ggggggaggg gagcaagccc ggcgaacgcc
     1141 ctgcggccct ctccaccatg gcgctgcccg agatcgtcga gcgcttcatc cacgtcgatg
     1201 acagcaccgg cgagttcgcc ttcgacctct ggacacagga gatggtcaag cgctccaaga
     1261 tcgtcgccat gctgccggcg cgcgtgcgct gggatgacgt caaggaccac cccacgtggc
     1321 agtcgcgcgc gacctacatc gacgaaatcg gcttcgaccc taccggccgc gacagcacga
     1381 tcaagctcaa caagtggaag ggatggccga cgaagccgcg cgacggcaag tgcgagctgc
     1441 tgctcgacct gctgcgctac ctctgcggca acgaggccaa cagcgacgag gtgtatcgct
     1501 ggatgttgtg ctggctggcc tacccgctgc agcacccggg cgccaagatg caaagcgcgg
     1561 tcgtcgtgca tggcccgcag ggcaccggca agagccgatt ctttgaggcc tacgccaaga
     1621 tctacgccca ccacgcggtc gtgctcacac agtccgcgct cgaggacaag ttcaacagcg
     1681 actgggggca aaagctgttc ggggtggccg acgaggtcgt cgcccgcggg gaaatgcacc
     1741 acgtgaagaa cttcctgaaa aacctgatca cgtccgaaac gatcaggatc aaccccaaga
     1801 acctgccggc ctacaccgag cgcaaccaca tcaacctcgt cttcctgtcc aacgagaagc
     1861 agccgatcgt cctcgaaaac gacgaccgcc ggcacctggt gatctggacg ccgggcaagc
     1921 tctccgaggt cttctacgac gaggtcgccg ccgagatcga agccggcggc atcgaggccc
     1981 tgcaccacca cctgctgcac ctcgacctgg gcgacttcaa gccctggacc cgccctccga
     2041 tgaccaaggc caaagcccag ctcatcgaca ccaaccgcga atccgtcgac cgcttcctgc
     2101 tcgactggca aagcggcgac atcgaaggca tgcccttctg cccttgcggc agcgccgacc
     2161 tctacaccgc ctacctgcag tgggcgcggc gcgagggcgt gcgcttcccg cgcgaatcca
     2221 accagttcgc cggccacgtc gaaaagctcc ccggctgggt caagggccat aaggaccgct
     2281 acgccgacac ctactacacc ggcaagcccg tccggcagcg cttcgtcatc ccggcgatcg
     2341 aggcgctgcc gcccgaacac gcccagcgca aggacgaaac gcagacgcag tggctcaccc
     2401 gctgctactt cgccttccgt gccgcgctgc aaggggggca gtcatgaccg cacaccgcac
     2461 cgcacgggct accgcacgcc ccaccgcacg cctgaaaccc gcgccaatac tggaaccgca
     2521 cggggcgcac gggcttacaa gcgcgcgtac acgtgcgcgc tcgcaaacat gctgcacgca
     2581 caaaaacagt tcacgtacac acgtaccccc cccgtgcgcc cgtgcgccac gtgcgctacg
     2641 cgggtttcag gcgtgcgccc acccgtgcgg tggcgtgcgg tacacccgtg cggtcgcggg
     2701 cgcgcgtttt cacccccacg cctgacgcct gaaaaaaaag aaagaggaag ccgagatgac
     2761 cctggagacc aaagccgcat tcgcccgccg gctcgactgg aaccgcagca ccgtcacccg
     2821 agccgcccag gccggccgcc tcgtgctatc cggcgccctg gtcgacgtcg aagccagcct
     2881 cgcccggctc aaagccaccg agggcggccg cgccgacgta gcggcccggc acgccgccga
     2941 aagggggcag ggtggggatg cacacgcccc cgcagccgaa aacaccgcca cggccccgca
     3001 cgcgccccgc agcgccgaaa acgaagccgc gcccacatcc gacaccgacg gcgccggccg
     3061 cgcccgctac aaggccatca cgctgcagtt cgagaaccag caaatcaagc tcggcatggc
     3121 catggcccgc ggcctgcgct tcaaccgcaa ggacgtggcc cgcgaagccg tagcgctcgg
     3181 cgcaatcgtc cgcagcgccg tcgagcgcgt catcgaccag accgcccccc gcctggcggt
     3241 gatgaacgcg cccgatcgcc ggcgcgagct gctcggcgcc gagctgcgcg ccgtgcgccg
     3301 cgccctgcgc gccgaattcc cccgcgcgct gcgccgcctg cgcagtgcca gccagcgagg
     3361 tgacgcatga ccgagctctt ctgccacgaa ggcaacgtcg gcgaattcaa cgccgcactg
     3421 cgcgaacacc ccgacctacg cgccttcgct gccgcgctcc accaagccgg catgatcgac
     3481 ggcctgcgcg gcgcccgcct gcgcccggcc gacgcgccgc ggcccgaaaa cctccagggc
     3541 gtcacccctg tgctatccga cgcagcagaa agccgcctcg ccgatttgtg gtggcagcgc
     3601 aaacaggaac ccaaggcatg aaaccgcaaa tcgaacacct ccccctcgac gccctcgtcc
     3661 cctacgcccg caacagccgc acccacagcc ccgagcaaat cgcccaggtc gccgccagca
     3721 tccgcgagtt cggctttacc aacccggtgc tgatcgacgg cgagggcggc atcatcgccg
     3781 gccacggccg cgtgatggct gcgcgccagc ttgggctatc cgaggtgccg tgcatccgcc
     3841 tgaaccgcat cggataagcg cacatggcct ctctcgccga cctgcaagcc gagcgcgaac
     3901 gcctgcgcgc cgcacaagca aaggcggact tcgagaagct gctcgccgac acctgcgcgc
     3961 ttgacgacgt gcgcgccgat gcggtggccg cccgcgcggt gctgctgcag acgctcgacg
     4021 cgctggtcgg ccgctggctg tcggcgatcg acgacgagca cgacgagacc cgcgtgcact
     4081 acctcatgtc ggacgtggcc cacgacgtgc tgcgcgagct cggcgaatcc gtccaggccg
     4141 ccaccacccg cctgccgatg ctcggcgagc gcatcgcccg cggcgccaag ccgcgcgacc
     4201 tgctcaccgt cagccagcac gccgaccgct accgcgtcat ccgcaccggc accaacgccc
     4261 ccggcccatg gcgcacggcg ctcaccccct acctgcgcga catcatggac gacctctccg
     4321 agcactcgcc cgtgcgtaag gtggtcttca tcaagagttc gggcgtgggc ggtaccgagg
     4381 cgatgtacaa ctggctcggc tacgtcatgc accacctgca gaacaaggat ctgctggtgg
     4441 tggtgccgac gctcgagctg cgcgaccgct cgttcaaccc gcgcctggcg aagatgatcg
     4501 acgaaagcga agcgctcgcc aatctcgtca cgactgccag ccgcaacaag gccaaccgcg
     4561 gcgacctgct cgaatacggc gctcgcgccc gcatcatcaa ggccggcgcc aacagcccgg
     4621 acagcctgcg atcggaccac gtgccgtatg tgatctgtga cgaggtcgac gcctt
//
LOCUS       DAOQBS010000182         4627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_19657_length_4627_cov_13.774934, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000182 DAOQBS010000000
VERSION     DAOQBS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19657_length_4627_cov_13.774934"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PKN25_14260"
     CDS             <1..801
                     /locus_tag="PKN25_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV92111.1"
                     /translation="PAFLSVNNIEVIYNHVILVLKGVSLSVQEGSIVALLGGNGAGKT
                     TTLRAVSSLLKGERGEVTKGSVDYNGERVDALTPADLVKRGVVQVMEGRHCFGHLTIE
                     ENLRTGAYTRSDGKAAVEETLEKVYNYFPRLKTRRNSQAAYTSGGEQQMCAIGRALMA
                     KPKLVLLDEPSMGLAPLIVEEVFEIVKDLNTKERVSFLLAEQNTMVALRYADFGYIME
                     NGRIVMEGAAKDLASNEDVKEFYLGLAGEGRKSFRDIKHYRRRKRWLS"
     gene            852..2087
                     /locus_tag="PKN25_14265"
     CDS             852..2087
                     /locus_tag="PKN25_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV92112.1"
                     /translation="MDFHDTRETRDPEARERELLSRLPAQIAHARAAAPAFGRLLADV
                     DPDSVTSREALATLPVTRKSELLELQKAARPFGGFSAVGWGAACRRVFASPGPLYEPE
                     GARPDYYRLARALFAAGFRAGDLVHNTFSYHFTPAGSMMETAAHALGCTVFPAGVGQT
                     EQQLAAIADLQPNAYVGTPSFLRILLDKADELGVRVSFTKAFVSGEAFPPSMRDALAA
                     RGIMAFQAYATADIGLIAYETPAREGMVVDEDVLLEIVRPGTGDPVAVGEVGEVVVTT
                     LNPDYPLIRFGTGDLSAVLPGDSPCGRTNVRIKGWMGRADQTTKVKGMFVHPGQIAQV
                     VRRHPEITRARLVVDNPDLADRMTLMCEAAGGGSEALAAAVAASIRELTKLRGEVAFL
                     PAGALANDGKVIDDVRTYE"
     gene            2170..3360
                     /locus_tag="PKN25_14270"
     CDS             2170..3360
                     /locus_tag="PKN25_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNV92113.1"
                     /translation="MNPASNAPARAPLAGVRILDLTRLLPGPLATQHLADYGAEVIKV
                     EDTGAGDYARTMGAMGGDTSWFYQLVNRNKRSLRLDLKQEAGRALFLRLVESADVVVE
                     GFRPGVMERLGLGYAVLAERNPRVVLCSISGYGQTGPYALRAGHDINYLGYAGVLDQI
                     GCAGGAPALSNLQIGDLLGGTMGAVMGILVALFDARRSGRGREVDVSMTDAAFAHMIF
                     PLAEVLAHGGVRPRGEDLLTGGVPCYGVYETADGRYMAVGALEEKFWGLLCTTLGRAD
                     LIPGHLATGADGARVRGELATIFRGRSQREWVEVFDPVDCCVTPVLRLEESLRDPQLR
                     ARGMVVETNGLCHAGAAVRLGGMPPIPDAPAPACAGADTDAILEALGVDAEELARLRG
                     AGVV"
     gene            complement(3376..3807)
                     /locus_tag="PKN25_14275"
     CDS             complement(3376..3807)
                     /locus_tag="PKN25_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92114.1"
                     /translation="MSNDQNAQDPLEFVRGMWNQMGFSLPGMVTPTLDVDELDKRITD
                     MRAVEGWLKMNLNMLQMSIQGLEMQRAALAAVKAMSQQAQQAGEGGGATEPGANPFAA
                     AAAMWPWNLMGAQAAATPAPATPAEAKEGARGTRRKPATDK"
     gene            complement(3848..>4627)
                     /locus_tag="PKN25_14280"
     CDS             complement(3848..>4627)
                     /locus_tag="PKN25_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV92115.1"
                     /translation="AHTDPLLLDRILANLLSNAIRHTPHGRVLVACRRRGDRLRVEVR
                     DNGPGIAPESQELIFHEFVQLDNPERGQDKGLGLGLAIVRRLSTLLRLPLDLRSRPGH
                     GTVFAVEVPAAQPEGASRAHTPGSFEGVRVQLAGADPAALEATAELLADWGCTVDDGT
                     RTAPADDRPHGTELLILAPVPAGTADGLVLLAALRMRPDLADVPAILLVVDSTDEQRD
                     RARALNAHLLARPLRPSRLRALMNRLLGARDGDGAQGSGIG"
BASE COUNT          675 a         1580 c         1688 g          684 t
ORIGIN      
        1 ccggccttcc tcagcgtcaa caacatcgag gtcatctaca accacgtcat cctggtgctc
       61 aagggcgtgt cgctgtcggt gcaggagggc agcatcgtcg ccctgctcgg tggcaacggc
      121 gccggcaaga ccaccacgct gcgcgcggtg agcagcctgc tcaagggcga gcgtggcgag
      181 gtgaccaagg gctcggtgga ttacaacggc gaacgcgtgg acgcgctgac gccggcggac
      241 ctcgtcaagc gcggcgtcgt ccaggtcatg gaggggcggc attgcttcgg ccacctcacc
      301 atcgaagaaa acctgcgcac cggcgcctac acccgcagcg acggcaaggc cgcggtcgaa
      361 gagacgctgg agaaggtcta caactatttc ccgcgcctga agacgcgacg gaacagccag
      421 gccgcctaca cctcgggtgg cgaacagcag atgtgcgcga tcggtcgtgc gctgatggcc
      481 aagcccaagc tggtgctgct cgacgagccc tcgatgggcc ttgcgccgct gatcgtggaa
      541 gaggtgttcg agatcgtcaa ggatctcaac accaaggaaa gggtgagctt cctgctcgcc
      601 gagcagaaca ccatggtcgc gctgcgctat gccgacttcg gctacatcat ggagaacggc
      661 cgcatcgtga tggaaggcgc cgccaaggac ctggccagca acgaggacgt caaggagttc
      721 tacctcggcc tggcgggcga gggccgcaag agcttccgcg acatcaagca ctatcgccgc
      781 cgcaagcgct ggctgagctg agcgccgccg ccgcgacatc acccgacaat gcatcgagca
      841 ggggagccga gatggatttc cacgacacgc gcgagacccg cgatcccgag gcgcgcgagc
      901 gcgagctgct gagccgcctg ccggcgcaga tcgcccatgc gcgcgccgcc gcgcccgcct
      961 tcggccgcct gctggccgac gtcgacccgg attcggtcac cagtcgcgag gcgctcgcca
     1021 cgctgccggt cacacgcaag tcggaactgc tcgagctgca gaaggcggcg cgccccttcg
     1081 gcggcttttc ggcggtgggc tggggggcgg cgtgcaggcg ggtgttcgcc tcgcccggcc
     1141 cgctctacga acccgaaggc gcgcggcccg actactaccg cctggcgcgc gcgctgttcg
     1201 ccgcgggctt ccgtgccggc gacctggtgc acaacacctt ctcgtaccac ttcacgcccg
     1261 ccggctcgat gatggagacg gccgcgcacg cgctcggctg caccgtcttt cccgccggcg
     1321 tcggccagac cgagcagcag ctcgcggcga tcgccgacct gcagcccaac gcctacgtgg
     1381 gcacgccgtc cttcctgcgc atcctgctcg acaaggcgga cgagctcggc gtgcgggtgt
     1441 cgttcaccaa ggccttcgtc tccggcgagg cctttccgcc cagcatgcgc gacgcccttg
     1501 ccgcgcgcgg catcatggcc ttccaggcgt atgccactgc agacatcggc ctgatcgcct
     1561 atgagacgcc ggcgcgcgag ggcatggtgg tggacgagga tgtgctgctc gagatcgtcc
     1621 gccccggcac cggggacccg gtggccgtgg gcgaggtggg cgaggtggtg gtgaccaccc
     1681 tcaaccccga ttacccgctg atccgcttcg gcaccggcga cctctccgcg gtgctgcccg
     1741 gggactcgcc ctgtgggcgc accaacgtgc gcatcaaggg ctggatgggg cgcgccgacc
     1801 agaccaccaa ggtcaagggc atgttcgtgc atccggggca gatcgcgcag gttgtgcgac
     1861 ggcaccccga gatcacgcgc gcgcgcttgg tggtagataa tcccgacctc gccgaccgca
     1921 tgacgctgat gtgcgaggcg gcaggtggcg gcagcgaggc cctcgccgct gccgtagccg
     1981 ccagcatccg cgagctgacc aagctgcgcg gcgaggtcgc cttcctgccc gcgggcgcgc
     2041 tcgccaacga cggcaaggtc atcgacgacg tgcgcaccta cgaatagccc cgaggaggcc
     2101 ctgtccctgc gtgtcccggt gcctcccgct ggcatactgc cgtcttcccg caaaacgatg
     2161 gacgcgcaca tgaaccccgc atcgaacgct cccgcacgcg cgccgctcgc cggcgtgcgc
     2221 atcctcgacc tcacccggct gctgcccggg ccgctcgcca cccagcacct ggccgactac
     2281 ggcgccgagg tgatcaaggt ggaagacacc ggtgccggcg actacgcgcg cacgatgggc
     2341 gcgatggggg gcgacaccag ctggttctac caactggtga accgcaacaa gcgcagcctg
     2401 cggctcgacc tcaagcagga ggcggggcgt gcgctgttcc tgcgtctggt ggaaagcgcc
     2461 gatgtggtgg tcgagggctt ccgcccgggg gtgatggagc gcctcggcct cggctacgcc
     2521 gtgctcgccg agcgcaatcc gcgcgtagtg ctgtgcagca tcagcggcta cggacagacc
     2581 gggccgtacg cactgcgtgc cggtcacgac atcaactatc tcggctacgc cggcgtgctc
     2641 gaccagatcg gctgcgccgg cggcgcgccg gcgctgtcga acctgcagat cggcgacctg
     2701 ctcggcggca ccatgggcgc ggtgatgggc atcttggtgg cgctgttcga cgcgcgccgc
     2761 agcggccgcg gccgcgaggt cgacgtgtcg atgaccgacg ccgcgttcgc gcacatgatc
     2821 ttcccgctcg ccgaggtgct tgcccacggt ggcgtgcggc cgcgcggcga ggacctgctc
     2881 accggcgggg tgccctgcta cggcgtgtat gagaccgcgg acggccgcta catggcggtg
     2941 ggtgcgctgg aggagaagtt ctgggggctg ctgtgcacta cgctcgggcg tgcggacctg
     3001 atcccggggc atctcgccac cggggcggac ggagcacgcg tgcggggcga gcttgcgacg
     3061 atcttccgcg gacgcagcca gcgcgagtgg gtggaggtgt tcgacccggt cgattgctgc
     3121 gtgacgcccg tgttgcgcct ggaggagagc ctgcgcgacc cgcagctgcg cgcacgcggc
     3181 atggtggtgg agacgaacgg cctgtgccac gccggcgcgg cggtgcgtct gggcgggatg
     3241 ccgccgatac ccgacgcgcc ggcaccggcg tgtgcggggg cggatacgga tgcgatcctg
     3301 gaggcgctcg gcgtggatgc cgaggagctt gcccggctgc ggggcgcggg cgtggtgtag
     3361 cccgcggcgg gtcgattact tgtcggtggc cggcttgcgg cgggtgccac gcgcgccttc
     3421 cttcgcttcg gccggggtcg cgggagcggg cgtggcggcc gcctgcgcgc ccatcaggtt
     3481 ccacggccac atcgccgccg cggcggcgaa gggattggct ccaggctcgg tcgcgccgcc
     3541 gccttcgcct gcctgctggg cctgttggct catcgccttg accgcggcga gcgcggcgcg
     3601 ctgcatctcg agtccctgga tgctcatctg cagcatgttc aggttcatct tgagccagcc
     3661 ctcgaccgcg cgcatgtcgg tgatgcgctt gtccagctca tcgacatcga gcgtgggcgt
     3721 gaccatgccg ggcagggaga aacccatctg gttccacatg ccgcgcacga actcgagcgg
     3781 atcctgggcg ttctggtcat tggacatcgg gctgctccgt tcggggggtt gaggtgacgg
     3841 aacaatatta cccgatgccg ctgccctgcg ctccatcgcc gtcgcgcgcg ccgagcaggc
     3901 ggttcatgag ggcgcgcagg cgcgaggggc gcagcggacg ggcgagcaga tgggcattca
     3961 gggcgcgggc gcgatcgcgc tgctcgtcgg tgctgtcgac gacgagcagg atcgcgggca
     4021 cgtcggccag gtcggggcgc atgcgcaggg ctgcgagcag gacaaggccg tcggccgtgc
     4081 cggcgggcac cggcgcgagg atcagcagct cggtgccgtg ggggcggtcg tccgccggcg
     4141 ccgtgcgggt gccgtcgtcc accgtgcagc cccagtcggc gagcagctcg gcggtcgcct
     4201 cgagggcggc gggatccgcg ccggcaagct gtacccggac cccttcgaag ctgcccggcg
     4261 tatgcgcccg ggaggcgcct tccggctgtg cggcggggac ctccaccgcg aacaccgtcc
     4321 cgtggccggg ccgcgagcgc aggtcgagcg gcagccggag cagggtgctg aggcggcgca
     4381 cgatcgccag cccgagcccg aggcccttgt cctggccgcg ctccggattg tcgagctgga
     4441 cgaactcgtg aaagatcagt tcctgcgatt ccggcgcgat gccggggccg ttgtcgcgta
     4501 cctccacgcg cagccggtcg ccgcgacgcc ggcaggcgac cagcacgcgg ccgtgcgggg
     4561 tgtggcggat ggcattcgac aacagattgg cgaggatgcg gtcgaggagc agcggatcgg
     4621 tgtgggc
//
LOCUS       DAOQBS010000183         4504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_20463_length_4504_cov_14.046752, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000183 DAOQBS010000000
VERSION     DAOQBS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20463_length_4504_cov_14.046752"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..>1294)
                     /locus_tag="PKN25_14285"
     CDS             complement(308..>1294)
                     /locus_tag="PKN25_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HNV92116.1"
                     /translation="LILHALVREPGAPSRLVLREAPCALDAAALRLAEHLCAQYESRS
                     AKGFGRFEEDEERFPLARWLREHLVDDTLDFTALSRQLAERVQAIADEEELEDGGHLV
                     LARIREGEGDSLWVALLGSSDGVAIGGDLAPLDCTHVDFSALHAAGRVDLAGWRRGDE
                     RYLGFLRGRGKGGAWFKRVLGCSDVMVALQETKKLVATLDRFVDTERLAPATRDAVLE
                     RAHDYLDTLGEAGAPVVFEELAREVFPEQPERLDALLRAEETKIAPGFVPNRRAIRPL
                     VRFSASAENWKLEFERSGLHSGAVHYDRATDTLVLSGVPDYLKRMLEEDAKG"
     assembly_gap    1295..1394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1549..2655
                     /locus_tag="PKN25_14290"
     CDS             1549..2655
                     /locus_tag="PKN25_14290"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HNV92117.1"
                     /translation="METTRTKLRGVNLGSWLLLEKWMVPSLFEGLEATDETTWCAELG
                     PAAAERLRRHWASFVTREDFAWIAARGLNAVRIPIGHWIFGPDYPYHPKYGAHRHPFV
                     TGGIEVLDRALDWAQEFGLRVIIDLHAAPGCQNGFDNGGIKDVVEWHTKKEYLEHSLA
                     VLERLAERYRAHPALHGIELLNEPRWDVPTDYLKSYYLEAYARIRKHCAPETVAVVFH
                     DGFRSFREYLGFMQAPAFRNVVFDYHRYQCFERGDIDMDIHGHIRKAAVDWREEADAI
                     NAELGLPAVCGEWSLGLDLKVVSLWAEGPFNHALEHMDDFQQDVASRAYGDSQLMTFE
                     RLAGWFFWSYKTETTPAWCLRECVERGWLPSRFG"
     gene            complement(2641..4449)
                     /locus_tag="PKN25_14295"
     CDS             complement(2641..4449)
                     /locus_tag="PKN25_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HNV92118.1"
                     /translation="MSSTHAASLPKQPHRRPPHRDGGRRFLLLAGGYWNCERKWQVRG
                     TVLALALLTMAQVGLAIWVSYWNRDLFDALEERALSELMKLVGVFVLIFALTMGVTAL
                     HMHVKRRLQLDWRRWLTDLLLEHWLAHGHLYRLQFTAGEHDNPDGRIAEDIRIATDVA
                     VSLAHSLLFALLILGSFIDILLRVSGSAPLPGTSIAVHGYMVLAAFLYAGVGAALGLM
                     LGRPLIRATNRLQTVEANLRFGLARAREHAEPIAMMHGEPHERREAARLFDDVGRGWN
                     RQTLAYLGIVSFSTGYGTLLPVFPILIAAPQYIAGAMTLGVLMQAAQAFQRLTTALSW
                     PIDNLGELARCRASIDRVASLYEDMLALERAEARSRGRERIAVCSSPHPELELRQLRL
                     QTPSGEILLDDFDLRVRRGERVLICGDPAVTVSLFKAVAGLWPWGSGEVRLPLGRDLM
                     FVPRQPFLPAGSLRMVLSYPDDADRHADAVLERALECAGIAWLAPKLDTQEDWGRELP
                     LRIQQRLGVARVFLHQPGWIFIEEATDALAPKEEVDTLELLHHELPSSTLLVISRRAR
                     QNHFFERRLLLQRPRPGLQPPTAPAARPETPSLPEA"
BASE COUNT          641 a         1509 c         1646 g          608 t
ORIGIN      
        1 tggtggcgct ggtcgcgctg atgtcgtgga agtggccgcc ctggctggtg gtggcggcgt
       61 gcggggtggc gggcggggtg ctgggggggc tggccgcgta gcgcgggacc gagagcccga
      121 tcgcgacttg cgtcgctccc acgagaggcc cgcaacgccc ggggtggagt cgttcccacg
      181 agaagcccgc aacgcccgag gtggaatgtg ggagcgacgc aagtcgcgat cgcggcggtg
      241 gaaggcgcgc ggcgacgatc gcgcgcgcaa acgctcgccg ccgtcctgcg cggccccggc
      301 ggtctcctca gcccttggcg tcttcctcca gcatgcgctt gaggtaatcc ggcacgcccg
      361 acagcaccag ggtgtcggtg gcgcggtcgt agtgcaccgc gccggaatgc aggccgctgc
      421 gctcgaactc cagcttccag ttctccgcgc tggcgctgaa gcgtaccagc gggcggatgg
      481 cgcgccggtt gggcacgaag ccgggggcga tcttcgtctc ctcggcgcgc agcagcgcgt
      541 ccaggcgctc gggctgttcg gggaagacct cgcgcgcgag ctcctcgaac accaccgggg
      601 cgccggcctc gccgagggtg tcgaggtagt cgtgggcgcg ttcgagcacg gcgtcgcggg
      661 tggcgggggc gaggcgctcg gtgtcgacga agcggtcgag ggtggcgacc agcttcttgg
      721 tctcctgcag cgccaccatc acgtcgctgc agccgagcac gcgcttgaac caggcgccgc
      781 ccttgccgcg cccgcgcagg aagccgaggt agcgctcgtc gccgcgccgc cagccggcga
      841 ggtcgacccg cccggcggcg tgcagggcgg agaagtccac atgggtgcag tcgagcggtg
      901 cgaggtcgcc gccgatcgcc accccgtcgc tgctgccgag cagcgccacc cacaggctgt
      961 cgccctcgcc ctcgcggatg cgcgcgagca cgagatggcc gccgtcttcc agctcttcct
     1021 cgtcggcgat cgcctgcacg cgctcggcga gctggcgcga cagggcggtg aagtcgagcg
     1081 tgtcgtcgac gaggtgctcg cgcagccagc gcgcgagcgg gaagcgctcc tcgtcctcct
     1141 cgaagcggcc gaagcccttg gcgctgcggc tctcgtactg cgcgcacagg tgctcggcca
     1201 ggcgcagcgc cgcagcgtcg agtgcgcagg gcgcctcgcg cagcaccagg cgcgagggtg
     1261 cgccgggctc gcgcaccagg gcgtggagga tcagnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnattgcc gccgcggcgt gggccggccc gtgggagcga cgcaagtcgc
     1441 gaaagggcgg cgcacggtgg gcgcgagctt tttcacggtc gggcgaggcg tttggctgcc
     1501 agaatgagcc atgtccatca cgcccgatcc gggcaagggg aggttctcat ggaaacgacg
     1561 cgtaccaagc tgcgcggggt gaacctgggt agctggctgc tgctggagaa gtggatggtg
     1621 cccagcctgt tcgagggcct ggaggcgacc gacgagacca cctggtgcgc cgagctcggt
     1681 cccgccgcgg ccgagcgcct gcgccggcac tgggcgagct tcgtgacccg cgaggacttc
     1741 gcctggatcg ccgcacgcgg cctcaacgcg gtgcgcatcc cgatcgggca ctggatcttc
     1801 ggccccgact acccctacca ccccaagtac ggcgcccacc gccacccctt cgtcaccggc
     1861 ggcatcgagg tgctcgaccg cgccctcgac tgggcgcagg agttcggcct gcgcgtgatc
     1921 atcgacctgc acgccgcgcc cggctgccag aacggcttcg ataacggcgg catcaaggac
     1981 gtggtggagt ggcatacgaa gaaggaatac ctcgagcact cgctcgccgt gctcgagcgc
     2041 ctggccgagc gctaccgcgc gcacccggcg ctgcacggca tcgagctgct caacgagccg
     2101 cgctgggacg tgccgaccga ctacctgaag tcctactacc tcgaggccta cgcgcgcatc
     2161 cgcaagcact gcgcgccgga gacggtggcg gtggtgttcc acgacggctt ccgcagcttc
     2221 cgcgagtacc tcggcttcat gcaggcgccg gccttccgca acgtggtgtt cgactatcac
     2281 cgctaccagt gcttcgagcg cggcgacatc gacatggaca tccacggcca catccgcaag
     2341 gcggcggtgg actggcgcga ggaagccgac gcgatcaacg ccgagctcgg cctgccggcg
     2401 gtgtgcggcg agtggagcct ggggctggac ctgaaggtgg tgtcgctgtg ggccgagggg
     2461 ccgttcaacc acgccctcga gcacatggac gacttccagc aggacgtggc cagccgcgcc
     2521 tacggcgaca gccagctgat gaccttcgaa cgcctggcgg gctggttctt ctggagctac
     2581 aagaccgaga ccacgccggc ctggtgcttg cgcgaatgcg tggagcgcgg ctggctgccc
     2641 tcacgcttcg ggtagggagg gcgtttccgg ccgcgccgcg ggcgcggtcg gcggctgcag
     2701 gcccgggcgc gggcgctgca gcagcagccg gcgctcgaag aagtggttct ggcgcgcgcg
     2761 ccggctgatc accagcaggg tcgagctggg cagctcgtgg tgcagcagct ccagggtgtc
     2821 gacctcttcc ttgggcgcga gcgcgtcggt ggcctcctcg atgaagatcc accccggctg
     2881 gtgcaggaac acgcgtgcga cgcccaggcg ctgctggatg cgcagcggca gctcgcgccc
     2941 ccagtcctcc tgggtgtcga gcttgggggc cagccaggcg atgccggcgc attcgagcgc
     3001 acgctcgagc acggcgtcgg cgtggcggtc ggcgtcgtcg ggatagctca gcaccatgcg
     3061 caggctgccc gcgggcagga agggctggcg cggcacgaac atcaggtcgc gcccgagcgg
     3121 cagcctcacc tccccgctgc cccacggcca cagcccggcc accgccttga acaggctcac
     3181 ggtgaccgca gggtcgccgc agatcagcac gcgctcgccg cggcgcacgc gcaggtcgaa
     3241 gtcgtcgagg aggatctcgc ccgacggtgt ctgcaggcgc agttggcgca gctcgagctc
     3301 ggggtggggc gagctgcaca ccgcgatgcg ctcgcggccg cggctgcggg cttcggcgcg
     3361 ctcgagcgcg agcatgtcct cgtagaggct ggcgacgcgg tcgatcgagg cgcggcagcg
     3421 cgccagctcg ccgaggttgt cgatcggcca cgacagcgcc gtggtcaggc gctggaaggc
     3481 ctgcgccgcc tgcatcagca cgcccagcgt catcgccccg gcgatgtact gcggcgcggc
     3541 gatcaggatg gggaacaccg gcagcagggt gccgtagccg gtggagaagg acacgatgcc
     3601 gaggtaggcc agggtctgcc ggttccagcc ccgcccgacg tcgtcgaaca ggcgcgcagc
     3661 ctcgcggcgc tcgtgcggct cgccatgcat catcgcgatc ggctcggcgt gctcgcgcgc
     3721 gcgcgccagc ccgaagcgca ggttggcctc gacggtctgc aggcggttgg tggcgcggat
     3781 cagcgggcga ccgagcatga gcccgagcgc cgcgcccacg cccgcataca ggaaggcggc
     3841 gagcaccatg tagccgtgca ccgcgatcga ggtccccggc agcggcgcgc tgcccgagac
     3901 gcgcagcagg atgtcgatga agctgcccag gatcagcagc gcgaacagca gcgagtgggc
     3961 gagcgagacc gccacgtcgg tggcgatgcg gatgtcctcg gcgatgcggc cgtcggggtt
     4021 gtcgtgctcg ccggcggtga actgcaggcg gtagaggtgg ccgtgcgcca gccagtgctc
     4081 gagcagcagg tcggtgagcc agcgccgcca gtcgagctgc agccggcgct tgacgtgcat
     4141 gtgcagcgcg gtcaccccca tggtgagcgc gaagatcagc acgaagaccc cgaccagctt
     4201 catcagctcg gacagcgcac gctcctcgag cgcgtcgaag aggtcgcggt tccagtagct
     4261 cacccagatc gccagcccga cctgcgccat cgtcagcagc gccagcgcca gcaccgtccc
     4321 gcgcacctgc cacttgcgct cgcagttcca gtatcccccc gccagcagca ggaagcgccg
     4381 cccgccgtca cggtggggcg gccggcggtg gggctgcttg ggaagggagg cggcgtgggt
     4441 ggaggacatg gagcaatcat gcctcgttac ggggcgttcg tccgtggagg aagacgcggt
     4501 ttgc
//
LOCUS       DAOQBS010000184         4412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_21110_length_4412_cov_13.224696, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000184 DAOQBS010000000
VERSION     DAOQBS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21110_length_4412_cov_13.224696"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..707)
                     /locus_tag="PKN25_14300"
     CDS             complement(171..707)
                     /locus_tag="PKN25_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNV92119.1"
                     /translation="MPTYSRRHTAALRSLPNTLPTLAWLLALTSCAWVAAELFWQLAA
                     PAPVAAVARHETDARKVAARIGLHVGRAAPVADKAPMRAAGPDARYTVTGIATGFGAL
                     PGFAILQAEDGSSLSLSPGQALPDGRRLVRLLPEAAEFELDGRRSTLALPARASERGE
                     SSRPPTPVADGGPRRDPR"
     gene            1093..3054
                     /locus_tag="PKN25_14305"
     CDS             1093..3054
                     /locus_tag="PKN25_14305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNV92120.1"
                     /translation="MSDPAPIFADDGPLAAAIPGFRARPQQIEMAQKIAEALRENRVL
                     VAEAGTGTGKTFAYLVPALLAGGKAIISTGTKTLQDQLFNRDLPTVRAALKVPVTIAL
                     LKGRANYVCHYHLERNARDGRFLTAQDAADLRAITRFARLTQTGDKAECTDVREDSLA
                     WLAATSTRDNCLGQDCPHKDECFVMQARRNAMEADVVVVNHHLFFADVMLRDEGMGEL
                     LPACNAVIFDEAHQLPETASLFFGDSVSTAQVLELARDTRSETVAAARDCVAMIDQTR
                     NLEKAARDLRLVFGPESARLSAAQAAEHENFDAMVEALEKALADFHAVLATQAERSEG
                     LGNCLRRTEEMSDRLASWRKPEDKELIRWVEVFTQSLALNATPLHVSDVFKRQLEGHP
                     RAWIFTSATLAVGKADFGHYCRELGLAWMDPPPLTAVWGSPFDYAEQALLYAPAGMPE
                     PNSPDYTERVAKVALPLIRAARGRAFVLCTSLRAMRRIHELILDGLAQSGDALPVLLQ
                     GEGSRTELLERFRRLGNAVLVASQSFWEGVDVPGDALSLVVIDKLPFAPPDDPVLAAR
                     VEHMQKQGLSPFVHHQLPKTVINMKQGAGRLIRSERDRGVLCICDPRMIDKSYGKVVW
                     RSLPPMRRTRAEADAVAFLESLPPPRQGG"
     gene            3131..3625
                     /gene="mobB"
                     /locus_tag="PKN25_14310"
     CDS             3131..3625
                     /gene="mobB"
                     /locus_tag="PKN25_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNV92121.1"
                     /translation="MKVFGFAGWSGSGKTTLVEKLIPEFTARGLRVSVIKHAHHGFDL
                     DKPGKDSWRHREAGAMQVLMLSNDRWVLMHELRGAPEPTLDEQLRLLEPCDLVLIEGY
                     KAAAVPKIEIHRPSHGKPPLWPENPHVVAVATNGEIDCPLPRLPLDEPAVVADFILDY
                     FKNR"
     gene            3622..>4412
                     /locus_tag="PKN25_14315"
     CDS             3622..>4412
                     /locus_tag="PKN25_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNV92122.1"
                     /translation="MNPDMLSFDEAYEKLLGFVRPVREVEEVDTLFAAGRVLAVDQHS
                     TIDQPPMDNSGMDGYALRAVDVPVAGTRLPVSQRIPAGSVGHELAPGTAARIFTGAPL
                     PPGADTVVMQELCEHAGDEVVVNTVPRAGEAVRRMGEDVARGARVLPAGLRLSPQAVA
                     LAASVGLARLPVHRRVKVAVFSTGSELVMPGEPLPPGGIYNSNRYLLRGLLAGLGCEV
                     ADFGIVPDRLDATREVLRRAAEGHDLILTSGGVSVGEEDHVKPAV"
BASE COUNT          648 a         1553 c         1596 g          615 t
ORIGIN      
        1 gtcgatctcg gcgttctgga agttgagcgc caccggctcg cccgcggcgt gcacgaggca
       61 cgagccgagg ccgagcgcgg caatggcgag cgcgcgcagc gcattgcgca acacggctcg
      121 gccccgcgcg gggaaggaac gaggaagcgg tcggagggtg gaaaggcggg tcatcgggga
      181 tcgcgtctgg gcccgccatc ggcgacgggc gtgggtgggc gggaggactc gccgcgctcg
      241 ctggcgcgcg cgggcagggc gagcgtgctg cggcggccat cgagctcgaa ttcggcggcc
      301 tcgggcagta ggcggacgag cctgcggcca tcgggcaggg cctggccggg gctcaggctg
      361 aggctggagc cgtcctcggc ctgcaggatc gcgaagccgg gcagggcgcc gaagccggtg
      421 gcgatgccgg tgaccgtgta gcgtgcatcc gggcctgccg cgcgcatcgg ggccttgtcg
      481 gcgaccggtg cggcacgacc cacatgcagg ccgatgcgtg cggccacctt gcgggcgtcg
      541 gtctcgtggc gcgcaaccgc agccaccggc gcgggcgccg caagttgcca gaacagctcc
      601 gccgccaccc aggcgcaaga ggtcagcgcg agcagccagg cgagcgtggg cagggtgttc
      661 ggcagggagc ggagggcggc ggtgtggcgg cgggaatacg tcggcatcgg caggagcggg
      721 gacactggtg aagaccgcag gcgcgatgtc cggcgcgcgc gacgggatcg cagcgacagg
      781 tcgtctgtcg ttcggcgcag ggcatgggac gccaccggaa tgcgcgtttt cgggaaagca
      841 ggcgccagtt cgcatgcgtg ggctggcgtc cgtcccctca tggggccatt cggggtgatc
      901 gccgttctgt cgctggcaaa ggctgtggac tatcggctgg agatgttgca gtggtgttgc
      961 cgtcgaccgg acccggggcg tgccgcgatc gcccgagcgg gccgtggagc ctcgtgggcg
     1021 cggcaagcga ataggctagt tgcaccgaaa ctgggataat gtacagcttg ccccggaagc
     1081 ccacgacgca tcatgtccga ccccgccccg atcttcgccg acgacggccc gctcgccgcc
     1141 gcgatccccg gttttcgtgc ccgtccgcag cagatcgaga tggcgcagaa gatcgccgag
     1201 gcgctgcgcg agaaccgcgt gctggtggcg gaggccggca ccggcaccgg caagaccttc
     1261 gcctacctgg tgccggcgct gctggccggg ggcaaggcca tcatctccac cggcaccaag
     1321 acgctgcagg accagctctt caaccgcgac ctgcccaccg tgcgcgccgc gttgaaggtg
     1381 ccggtgacga tcgcgctgct gaaggggcgc gccaactacg tctgccacta ccacctcgag
     1441 cgcaacgcgc gcgacggccg cttcctcacc gcgcaggacg ccgcagacct gcgcgcgatc
     1501 acgcgcttcg ccaggctcac gcagaccggc gacaaggccg aatgcaccga cgtgcgcgag
     1561 gactcgctgg cctggctcgc cgcgacctcg acacgcgaca actgcctcgg ccaggactgc
     1621 ccgcacaagg acgagtgctt cgtgatgcag gcgcggcgca acgcgatgga ggccgacgtg
     1681 gtggtggtca atcaccacct cttcttcgcc gacgtgatgt tgcgcgacga gggcatgggc
     1741 gagctgctgc cggcgtgcaa cgcggtgatc ttcgacgagg cccaccagct ccccgagacc
     1801 gcgagcctgt tcttcggcga cagcgtgtcc accgcccagg tgctggagct ggcgcgcgat
     1861 acgcgctccg agaccgtggc cgcggcgcgc gactgcgtgg cgatgatcga ccagacccgc
     1921 aacctggaaa aggccgcacg cgacctgcgc ctggtgttcg ggccggagag cgcgcggctc
     1981 tccgccgcgc aggccgccga gcatgagaac ttcgacgcca tggtcgaggc gctggagaag
     2041 gcgctcgccg acttccacgc ggtcctcgcc acccaggccg agcgctccga gggcctgggc
     2101 aactgcctgc gccgcaccga ggagatgtcc gaccgcctgg cgagctggcg caagcccgaa
     2161 gacaaggagc tgatccgctg ggtcgaggtc ttcacccagt cgctcgcgct caatgccacc
     2221 cccctgcacg tgtcggacgt gttcaagcgc cagctcgaag gccacccgcg cgcgtggatc
     2281 ttcacctcgg cgacgctggc ggtgggcaag gccgacttcg gtcactactg ccgcgagctg
     2341 ggcctggcct ggatggaccc gccgccgctc accgcggtgt ggggcagccc cttcgactac
     2401 gccgagcagg cgctgctgta cgcgccggcc ggcatgcccg agcccaactc gcccgactac
     2461 accgagcgtg tcgcgaaggt cgcgctgccg ctgatccgcg ccgcaagagg gcgcgccttc
     2521 gtgctgtgca cctcgctgcg tgcgatgcgc cgcatccacg agctgatcct cgacgggctt
     2581 gcgcagagcg gtgacgcgct gccggtgctg ctgcagggcg agggttcgcg caccgagctg
     2641 ctcgagcgct tccgccgcct gggcaacgcg gtgctggtgg cgagccagag cttctgggag
     2701 ggcgtcgacg tgccgggcga cgcgctctcg ctggtggtga tcgacaagct gcccttcgcg
     2761 ccgcccgacg acccggtgct ggcggcgcgc gtcgagcaca tgcagaagca gggcctgagc
     2821 cccttcgtgc atcaccagct gcccaagacc gtgatcaaca tgaagcaggg cgccgggcgc
     2881 ctgatccgca gcgagcgcga ccgcggcgtg ctgtgcatct gcgacccgcg catgatcgac
     2941 aagtcctacg gcaaggtcgt ctggcgcagc ctgccgccca tgcgccgcac gcgcgccgaa
     3001 gccgacgcgg tcgccttcct cgagagcctg ccgccgccgc ggcagggcgg ctgagctggg
     3061 tcaagggtgg gcagcggcgc tctgttgtat ccttcggccc tgctcatatc acgccgtggg
     3121 gcgagtctcg atgaaagtct tcggtttcgc cggctggtcc ggctccggca agaccaccct
     3181 ggtcgaaaag ctgatccccg aattcaccgc gcgcggtctg cgcgtgtcgg tgatcaagca
     3241 cgcccaccac ggcttcgacc tcgacaagcc gggcaaggac tcgtggcgcc accgcgaggc
     3301 cggcgcgatg caggtgctga tgctgtcgaa cgaccgctgg gtgctgatgc acgagctgcg
     3361 cggcgccccc gagcccacgc tggacgagca gctgcgcctg ctcgaaccct gcgacctggt
     3421 gctgatcgaa ggctacaagg cggcggcggt acccaagatc gagatccacc gcccctcgca
     3481 cggcaagccg ccgctgtggc ccgagaaccc gcacgtggtc gcggtcgcca ccaacggcga
     3541 gatcgactgt ccgctgccgc gtctgccgct cgacgagccc gccgtggtcg ccgacttcat
     3601 cctggactac ttcaagaacc gatgaacccc gacatgcttt ccttcgacga ggcctacgag
     3661 aagctgctcg gcttcgtccg cccggtgcgc gaggtggagg aggtggacac cctgttcgcc
     3721 gccggccgcg tgctcgcggt cgatcagcat tccaccatcg accagccgcc gatggacaac
     3781 tccggcatgg acggctacgc cctgcgcgcc gtcgacgtgc cggtggccgg aacccgcctg
     3841 ccggtgagcc agcgcatccc cgccggcagc gtcggccacg agctcgcccc cggcaccgcc
     3901 gcgcgcatct tcaccggcgc gccgctgccc cccggcgcgg ataccgtggt gatgcaggag
     3961 ctgtgcgagc acgccggcga cgaggtggtg gtcaacacgg tgccgcgtgc cggcgaggcg
     4021 gtgcgtcgca tgggcgagga tgtcgcccgc ggcgcgcgcg tgctgcccgc cgggctgcgc
     4081 ctgagcccgc aggcggtcgc gctcgccgcc tcggtgggtc tggcgcggct gccggtgcat
     4141 cggcgcgtca aggtcgccgt cttctcgacc ggctccgagc tggtgatgcc gggcgagccc
     4201 ctgccgcccg gcggcatcta caactccaac cggtacctgc tgcgcggcct gctcgccggg
     4261 ctaggctgcg aggtcgcgga cttcggcatc gtgcccgacc gcctcgacgc cacccgcgag
     4321 gtgctgcgcc gcgcggccga gggacacgat ctcatcctca ccagcggcgg cgtgtcggtg
     4381 ggcgaggaag accacgtcaa gccggcggtc ga
//
LOCUS       DAOQBS010000185         4332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_21735_length_4332_cov_18.332008, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000185 DAOQBS010000000
VERSION     DAOQBS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21735_length_4332_cov_18.332008"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PKN25_14320"
     CDS             complement(<1..545)
                     /locus_tag="PKN25_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNV92123.1"
                     /translation="MLANILLLVIDVAASFLTLMLLARFFMQWQRISFRNQLGQFVVS
                     TTDWIVRPLRRFIPGLFGLDMASLLPAWLVQVLLVMFELSLRGAAFSGNTAAVLAGLW
                     GVGLIELLRMMIYLVFAVVLGSAVLSWVSPHAPLAPLLHSIAAPFLRPFRRVIPSIAN
                     VDLSPLVLLLVLQIVLMVLAGA"
     gene            complement(549..1373)
                     /gene="proC"
                     /locus_tag="PKN25_14325"
     CDS             complement(549..1373)
                     /gene="proC"
                     /locus_tag="PKN25_14325"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNV92124.1"
                     /translation="MKITFLGGGNMATALIGGMLERGFAAAGIQVVELGEAARDALAQ
                     RFGVRAVESFDDAALACDVLVLAVKPQQMKAALAPIAGRLCGQLVISIAAGLRLADLG
                     RWLGAPGAPHARLVRCMPNTPALIGAGVTGLYADPSVDAAGRETAGRILGAVGSTVWA
                     DDEAQMDAVTAISGSGPAYVFHFIEALESAGRSLGFDEAGARRLAIDTVLGAARLAAG
                     SEDAPAVLRQKVTSKGGTTEAALASLAAAGWHDELVAAVQAAEARGRELGAELGKD"
     gene            complement(1417..2130)
                     /locus_tag="PKN25_14330"
     CDS             complement(1417..2130)
                     /locus_tag="PKN25_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNV92125.1"
                     /translation="MTTIAARLDAVRARIAAAARHAGRDPADVKLLAVSKTWPAEAVR
                     EAAAAGQRAFGENYVQEGVDKVEALRALGLEWHFIGPLQSNKTRPVANAFDWVHGIDR
                     LKIAERLSAQRDVHLPPLDVCIQVNVSGEDSKSGVAPDEAGALAHAVAALPRLRLRGL
                     MCIPEPSADEAVLRARFAVLRGLHDELRAAGLALDTLSMGMSHDLEPAIAEGANIVRV
                     GTAIFGERARTAPTPTPTA"
     gene            2257..3300
                     /locus_tag="PKN25_14335"
     CDS             2257..3300
                     /locus_tag="PKN25_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNV92126.1"
                     /translation="MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEH
                     KEVHAMVYDIMNDAQRKQLEETWECDFSFAVPNLARFRVNAFQQNRGAGAVFRTIPSK
                     VLTLEELNCPKIFKEIAHQPRGIVLVTGPTGSGKSTTLAAMLDYVNENEYGHILTVED
                     PIEFVHESKRCLVNQREVHRDTMSFNNALRAALREDPDVILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTIDRIVDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGQG
                     RVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQNIGMQTLDQCLVDLVRRNVVSAAE
                     ARVKAQNKDSFAA"
     gene            3342..>4332
                     /locus_tag="PKN25_14340"
     CDS             3342..>4332
                     /locus_tag="PKN25_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNV92127.1"
                     /translation="MERDQALKFMHDLLRLMLQKNGSDLFITAGFPPAIKVDGRIVPQ
                     SNQILQPQHTTELARAIMNDRQAADFETHKECNFGISPAGIGRFRANAFVQQGRVALV
                     LRTIPQKIPNFKELGLPPVLTEIALAKRGLVIFVGGTGTGKTTSLASLVDYRNENTYG
                     HIITIEDPIEFVHQHKNCIVSQREIGIDTESWMSALKNTLRQAPDVILMGEIRDRETM
                     DYAIAFAETGHLCLATLHANSANQAIDRIINFFPEDRRQQLLMDLSLNLRAMVSQRLL
                     PMKDRKGRVPAVEVMLNSPLIADLVFKGNVPEIKDVMKRSRELGMQTFDQSLFQ"
BASE COUNT          735 a         1566 c         1442 g          589 t
ORIGIN      
        1 gcgccggcga gcaccatcag cacgatctgc agcaccagca gcagcaccag cggcgacagg
       61 tcgacgttgg cgatgctcgg gatcacgcgc cggaacgggc gcaggaaggg cgcggcgatc
      121 gagtgcagca gcggcgccag cggcgcgtgc gggctcaccc aggacagcac cgccgagccc
      181 agcaccaccg cgaacaccag gtagatcatc atgcgcagca gctcgatcag gcccaccccc
      241 cacaggccgg cgagcacggc ggcggtgttg ccgctgaagg ccgcgccgcg caggctgagc
      301 tcgaacatca ccagcagtac ctgaaccagc caggcgggca gcaggctcgc catgtcgaga
      361 ccgaacagcc cggggatgaa gcggcgcagc gggcgcacga tccagtcggt ggtgctgacc
      421 acgaactggc cgagctggtt gcggaaggag atgcgctgcc actgcatgaa gaagcgcgcg
      481 agcagcatca gcgtgaggaa gctggcggcg acgtcgatca ccagcagcag gatgttggcg
      541 agcatggttc agtccttgcc gagttcggcg ccgagctcgc gcccgcgcgc ttcggccgcc
      601 tgcaccgcgg cgacgagttc gtcgtgccag ccggccgccg ccaggctggc gagcgcggcc
      661 tcggtggtgc cgcccttgga ggtgaccttc tggcgcagca cggcgggggc gtcttccgag
      721 cccgccgcca gcctcgccgc gccgagcacg gtgtcgatcg ccaggcggcg cgcgccggcc
      781 tcgtcgaagc cgaggctgcg cccggcggat tcgagcgcct cgatgaagtg gaagacgtag
      841 gccgggccgc tgcccgagat cgcggtgacc gcgtccatct gcgcctcgtc gtcggcccac
      901 accgtgctgc cgaccgcgcc caggatgcgc ccggcggtct cgcggccggc ggcgtccacc
      961 gaagggtcgg cgtacaggcc ggtgacgccg gcgccgatca gcgccggtgt gttgggcatg
     1021 cagcgcacca ggcgggcatg gggcgcaccc ggcgcaccca gccagcgccc gaggtcggcc
     1081 aggcgcaggc cggcggcgat gctgatgacg agctggccgc acaggcgccc ggcgatcggt
     1141 gccagcgcag ccttcatctg ctgcggcttg accgcgagca cgagcacgtc gcaggcgagc
     1201 gccgcgtcgt cgaaggactc gaccgcacgc acgccgaagc gctgcgcgag cgcgtcgcgc
     1261 gccgcctcgc cgagttcgac gacctggatg ccggcggcgg cgaagccgcg ctcgagcatg
     1321 ccgccgatga gggcggtggc catgttgccg ccgccgagga aggtgatctt catggatgga
     1381 cctgctggtt ggggccggag cacacgcgga cgcgcgtcag gccgtagggg tcggggtcgg
     1441 cgcggttcgg gcacgctcgc cgaagatcgc cgtgcccacg cgcacgatgt tcgcgccctc
     1501 ggcgatcgcc ggctcgaggt cgtgcgacat gcccatcgac aaggtgtcga gcgcgagtcc
     1561 ggcggcgcgc agctcgtcgt ggagcccgcg cagcacggcg aagcgcgcgc gcagcacggc
     1621 ctcgtccgcg ctcggttcgg ggatgcacat gaggccgcgc aggcgcagcc gcggcagggc
     1681 ggcgaccgcg tgcgcgagcg cgccggcctc gtccggcgcc acgccgctct tgctgtcctc
     1741 gccgctgaca ttgacctgga tgcacacgtc gagcggcggc agatgcacgt cgcgctgcgc
     1801 cgacaggcgc tcggcgatct tcaggcggtc gatgccatgc acccagtcga aggcgttcgc
     1861 caccgggcgc gtcttgttgc tctgcagcgg gccgatgaaa tgccattcga gcccgagcgc
     1921 gcgcagcgcc tcgaccttgt ccacgccttc ctgcacgtag ttctcgccga aggcacgctg
     1981 cccggcggcg gcggcctcgc gcaccgcctc cgccggccag gtcttgctca ccgcgagcag
     2041 cttcacgtcc gcagggtcgc gcccggcatg tcgtgcggcg gcggcgatgc gggcgcgcac
     2101 cgcgtcgagg cgtgcagcga tcgtcgtcat ggtccgtggc ctatttcctg ggatttcggg
     2161 gcggcaaaag tatagcatcg ctccatcgcc caccccgagg ccgaccccga gcgccgcccg
     2221 cgcaggcggc cgtgtcgcca ttcgcgccgg aataccatgg acatcaccga actgctcgcc
     2281 ttcgcggtca agaacaaggc ctccgacctc cacctctccg ccggcctgcc gccgatgatc
     2341 cgcgtgcacg gcgacgtgcg ccggatcaac ctgccgccgc tcgagcacaa ggaggtgcat
     2401 gccatggtgt acgacatcat gaacgacgcg cagcgcaagc agctcgagga gacctgggag
     2461 tgcgacttct ccttcgccgt gcccaacctc gcgcgcttcc gcgtcaacgc cttccagcag
     2521 aaccgcggcg caggcgcggt gttccgcacc attccgagca aggtgctgac cctggaggag
     2581 ctcaactgcc cgaagatctt caaggagatc gcccaccagc cgcgcggcat cgtgctggtg
     2641 accgggccga ccggctcggg caagtccacc acgctggcgg cgatgctcga ctacgtgaac
     2701 gaaaacgagt acggccacat cctcaccgtg gaggacccga tcgagttcgt gcacgaatcc
     2761 aagcgctgcc tggtgaacca gcgcgaggtg caccgcgaca ccatgtcctt caacaacgcg
     2821 ctgcgcgctg cgctgcgcga ggaccccgac gtgatcctgg tgggcgagat gcgcgacctc
     2881 gagaccatcc gcctcgccct caccgccgcc gagaccggcc acctggtgtt cggcaccctg
     2941 cacacctcat ccgcggccaa gaccatcgac cggatcgtcg acgtcttccc cgcggccgag
     3001 aaggacatgg tgcgctcgat gctttcggaa tccctgcgcg cagtgatctc gcagaccctg
     3061 ctcaagacca aggacggcca gggccgggtc gcggcgcacg agatcatgat cggcaccccg
     3121 gcgatccgca acctcatccg cgagaacaag atcgcgcaga tgtattcctc gatccagacc
     3181 ggccagaata tcggcatgca gaccctcgac cagtgcctcg tcgacctggt ccggcgcaac
     3241 gtcgtgtcgg ccgcggaagc acgcgtgaag gcgcagaaca aggacagctt cgccgcctga
     3301 gcggcatcca cccgcccgtc ccgggcagga gcacgcagac catggaacgc gaccaggccc
     3361 tcaagttcat gcacgacctg ctgcggctga tgctgcagaa gaacggctcg gacctcttca
     3421 tcaccgccgg cttcccgcct gcgatcaagg tcgacggccg catcgtgccg cagtcgaacc
     3481 agatcctgca gccgcagcac accaccgagc tggcgcgcgc gatcatgaac gaccgccagg
     3541 ccgccgactt cgagacccac aaggagtgca acttcggcat ctcgccggcg ggcatcggcc
     3601 gcttccgcgc caatgccttc gtgcagcagg gccgcgtcgc cctggtgctg cgcaccatcc
     3661 cgcagaagat ccccaatttc aaggagctcg gcctgccgcc ggtgctcacc gagatcgcgc
     3721 tcgccaagcg cgggctggtg atcttcgtcg gcggcaccgg caccggcaag accacctcgc
     3781 tcgcatccct ggtcgattac cgcaacgaga acacctacgg ccacatcatc accatcgagg
     3841 acccgatcga gttcgtgcac cagcacaaga actgcatcgt gtcccagcgc gagatcggca
     3901 tcgacaccga gtcctggatg tctgcgctca agaacaccct gcgccaggcg cccgacgtga
     3961 tcctgatggg cgagatccgc gaccgcgaga ccatggacta cgcgatcgcc ttcgccgaga
     4021 ccggccacct gtgcctggcc accctgcacg ccaacagcgc caaccaggcg atcgaccgca
     4081 tcatcaactt cttcccggaa gaccgccgcc agcagctgct gatggacctg tcgctgaacc
     4141 tgcgcgcgat ggtctcgcag cgcctgctgc cgatgaagga ccgcaagggc cgcgtaccgg
     4201 cggtggaggt gatgctcaac tcgccgctga tcgccgacct ggtcttcaag ggcaacgtgc
     4261 ccgagatcaa ggacgtgatg aagcgctcgc gcgagctcgg catgcagacc ttcgaccagt
     4321 cgctgttcca gc
//
LOCUS       DAOQBS010000186         4321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_21817_length_4321_cov_11.923113, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000186 DAOQBS010000000
VERSION     DAOQBS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21817_length_4321_cov_11.923113"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..552)
                     /locus_tag="PKN25_14345"
     CDS             complement(127..552)
                     /locus_tag="PKN25_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase subunit alpha"
                     /protein_id="HNV92128.1"
                     /translation="MIITGFQSLLDAARQQPEPQRLLLVFTRAELPEKATAEQRAKFE
                     QGEGGALVPVMYVDKKPDELQSFEELVEESRHTGQSWDMMFVGCLSGQGGREPSDDTV
                     QKALETMIMSIQGGIVAHLLAYRPNGEQVWLPDQAEGLA"
     gene            complement(694..2079)
                     /gene="cysS"
                     /locus_tag="PKN25_14350"
     CDS             complement(694..2079)
                     /gene="cysS"
                     /locus_tag="PKN25_14350"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNV92129.1"
                     /translation="MLSIYNTLSRSKEAFTPIEPGKVRMYVCGMTVYDFCHLGHARVM
                     VVFDMVARWLRASGLDVTYVRNITDIDDKIIRRAQENGESIRQLTDRFIAAMHEDADA
                     LGVLRPDHEPRATDYVAQMQSLISRLEQKGLAYVAGNRDVCYAVRKFDGYGKLSGKSL
                     DELRAGERVDVAQDKNDPLDFVLWKHAKVEEPGEVKWASPWGEGRPGWHIECSAMSSD
                     LLGDHFDIHGGGMDLQFPHHENEIAQSEGAHGHAFVNYWMHNGFVRVDDEKMSKSLGN
                     FFTIREVLQKYDPEVVRFFILRAHYRSALNYSDAHLEDARNALTRLYTALKNVPVAGE
                     PVVDWSEPFAQRLRAAMDDDFNTAEAVAVLFELANEVNRSAAPALAAQLKALGGVLGL
                     LGREPQAFLQAGAPDADGLDADAIEAKIAERAAAKKAKNFAEADRIRAELTEAGVILE
                     DGPGGTTWRRA"
     gene            2371..2958
                     /locus_tag="PKN25_14355"
     CDS             2371..2958
                     /locus_tag="PKN25_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV92130.1"
                     /translation="MKRHLLAFATAASLAFPVLAANPTVELKTSQGDIVVEVFADKAP
                     KSAENFVQYVKDGFYDGTVFHRVIDGFMVQGGGFDASMNQKATRAPIENEAKNGLKNE
                     RGTLAMARTSDPHSASAQFFINLVPNTFLDYPSRDGWGYAVFGKVVKGMEVVDKIAKL
                     PTANRGFHQNVPVEAIVVESARVVGTAAPAKKPAK"
     gene            3040..3534
                     /locus_tag="PKN25_14360"
     CDS             3040..3534
                     /locus_tag="PKN25_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV92131.1"
                     /translation="MAVKLHTNHGVITLELDAEKAPVTVANFLAYVEAGHYDNTVFHR
                     VIDGFMIQGGGFEPGMKQKATGEQIKNEADNGLKNDRGTIAMARTQAPHSATAQFFIN
                     VADNAFLNHTSPDLQGWGYCVFGKVSEGMDVVDSIRKVKTGSSGFHQDVPKEDVIIER
                     AEVV"
     gene            3603..>4321
                     /locus_tag="PKN25_14365"
     CDS             3603..>4321
                     /locus_tag="PKN25_14365"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNV92132.1"
                     /translation="MPEAARAALPALFISDLHLTEDEPANAAAFLDFLHGPARAAASL
                     FILGDLFEYWAGDDDLETPFNARIAAAIRQLADAGTAVFFMTGNRDLLAGPTFAAAIG
                     ATLLEDPARVRCGDRDEADAADAPVLLLAHGDALCTDDLAYQAFRRQVRDPAWQAGFL
                     AQPLAARKAFIAGLRQKSEAAKAEKAMEIMDVNADAVAALLREHGYPTLVHGHTHRPD
                     THRIRVDGHDCIRRVLPDWRG"
BASE COUNT          675 a         1606 c         1389 g          651 t
ORIGIN      
        1 acgtcgatcg cgacttgcgt cgctcccacg gaaaccgtca cgccggccaa cgcccgatgc
       61 gtcccccgta ggagcgacgc aagtcgcgat ttcagccgcc caacgaccgc cgcgcgactg
      121 agaggctcag gccagccctt cggcctggtc gggcagccag acctgctcgc cgttcgggcg
      181 gtaggccagc aggtgggcga cgatgccgcc ctggatcgac atgatcatgg tctcgagcgc
      241 cttctgcacc gtatcgtcgc tcggctcgcg cccgccctgc ccgctcaggc aaccgacgaa
      301 catcatgtcc cagctctgcc cggtgtggcg cgactcctcg accagttcct cgaagctctg
      361 cagctcgtcg ggcttcttgt ccacatacat caccggaacc agcgccccgc cctcgccctg
      421 ctcgaacttg gcgcgctgct cggcggtggc cttctcgggc agttcggcgc gagtgaagac
      481 gagcagcagg cgctgcggtt cgggttgctg gcgcgcggcg tcgagcaggc tctgaaagcc
      541 ggtgatgatc atgtgcgggg ttccttgtac gggttcggat cgtttggatt caactcggcc
      601 agccacggca gcgcctggcg ataccgcggt gacctgcgcc acagcacagc gctgatgccg
      661 accagcgcag tcgccccgct ggccgctgca ggctcaggcg cggcgccagg tggtgccgcc
      721 ggggccgtcc tcgaggatca cgccggcctc ggtcagctct gcgcggatgc ggtcggcctc
      781 ggcgaagttc ttcgccttct tggcggccgc gcgctcggcg atcttcgcct cgatcgcgtc
      841 ggcgtccagc ccgtccgcgt cgggcgcacc cgcctgcagg aaggcctgcg gttcgcgtcc
      901 caacaggccg agcacgccgc cgagcgcctt cagttgcgcg gcgagcgccg gcgcggcgct
      961 gcggttgacc tcgttggcga gctcgaacag taccgccacc gcttcggcgg tgttgaagtc
     1021 gtcgtccatc gccgcgcgca ggcgctgcgc gaagggctcg ctccagtcga ccaccggctc
     1081 accggccacc ggcacgttct tgagcgcggt gtacaggcgg gtgagcgcgt tgcgggcgtc
     1141 ctcgaggtgg gcgtcggagt agttgagcgc gctgcggtag tgcgcgcgca ggatgaagaa
     1201 gcgcaccacc tccggatcgt acttctgcag cacctcgcgg atggtgaaga agttgccgag
     1261 cgacttcgac atcttctcgt cgtcgacgcg cacgaagccg ttgtgcatcc aatagttgac
     1321 gaaggcatgg ccgtgcgcgc cttcggactg ggcgatctcg ttctcgtggt gcggaaactg
     1381 caggtccatg ccgccgccgt ggatgtcgaa gtggtcgccg agcaggtccg agctcatcgc
     1441 cgagcattcg atgtgccagc ccggccggcc ctcgccccag ggcgaggccc acttcacctc
     1501 gcccggctcc tcgaccttgg cgtgcttcca caacacgaag tcgagcgggt cgttcttgtc
     1561 ctgcgctaca tccacacgct cgccggcgcg caactcgtcg agcgacttgc ccgacagctt
     1621 gccgtagccg tcgaacttgc gcaccgcgta gcacacgtcg cggttgccgg ccacgtaagc
     1681 caggcccttc tgctccaggc gcgagatcag cgactgcatc tgcgcgacgt agtcggtggc
     1741 gcgcggctcg tggtcggggc gcagcacgcc gagcgcgtcg gcgtcctcgt gcatcgcggc
     1801 gatgaagcgg tcggtgagct ggcggatgct ctcgccgttc tcctgcgcgc ggcggatgat
     1861 cttgtcgtcg atgtcggtga tgttgcgcac gtaggtcacg tccagcccgc tcgcgcgcag
     1921 ccagcgcgcc accatgtcga acaccaccat cacgcgcgca tggccgaggt ggcagaagtc
     1981 gtacaccgtc atgccgcaga cgtacatgcg gaccttgccg ggttcgatgg gcgtgaaggc
     2041 ttccttgctg cgcgacaggg tgttgtagat ggagagcatc ggtgcggacg ggtcgttgct
     2101 ggaggccggc cgcaggcgga acgcgggcgg cggctcgatt gtgagggggg gggcttgttg
     2161 ctagaatccg gcgctcgagg cggacttcgg caggcccacg caagcgcgcg agtatagcac
     2221 gccgcctccg cccccttccc cgggcccggc cgcccggcgt gcatcgggag cgcatgcggc
     2281 acgcgcatcg ggcttgcccg gagcgcccgc gtcctgccag ctctccagcc atcccggcgg
     2341 ccatccttcc gccccattcc aggagtcccc atgaaacgac acctgcttgc cttcgccacc
     2401 gcggccagcc tagccttccc ggtgctcgcc gccaacccga cggtcgagct caagaccagc
     2461 cagggcgata tcgtcgtcga ggtcttcgcc gacaaggcgc ccaagtccgc cgagaacttc
     2521 gtgcagtatg tgaaggacgg cttctacgac ggcaccgtgt tccaccgcgt gatcgacggc
     2581 ttcatggtgc agggcggcgg cttcgacgcg agcatgaacc agaaggccac gcgcgccccg
     2641 atcgagaacg aggccaaaaa cggcctcaag aacgagcgcg gcaccctggc gatggcgcgc
     2701 acctccgacc cgcactcggc gagcgcgcag ttcttcatca acctcgtccc caacaccttc
     2761 ctcgactacc cgtcgcgcga cggctggggc tacgcggtgt tcggcaaggt cgtcaagggc
     2821 atggaggtcg tcgacaagat cgccaagctg cccaccgcca accgcggctt ccaccagaac
     2881 gtcccggtcg aggcgatcgt cgtcgagagc gcgcgcgtgg tcggcaccgc cgcgccggcg
     2941 aagaagcctg cgaagtaagc ccgcgaagcg aacccacacc ccccctgccc ggcgaccacg
     3001 ctgccgggcc cacaccggac ccaccccagg agaaccccca tggcagtcaa gctgcacacc
     3061 aaccacggcg tcatcaccct cgaactcgac gccgagaagg cccccgtcac cgtggccaac
     3121 ttcctcgcct acgtcgaggc cggccactac gacaacaccg tcttccaccg cgtcatcgac
     3181 ggcttcatga tccagggcgg cggcttcgag cccggcatga agcagaaggc cactggcgag
     3241 cagatcaaga acgaggccga caacggcctc aagaacgacc gcggcaccat cgccatggcg
     3301 cgcacccagg ccccgcactc ggccaccgcg cagttcttca tcaacgtcgc cgacaacgcc
     3361 ttcctcaacc acacctcgcc cgacctgcag ggctggggct actgtgtgtt cggcaaggtc
     3421 agcgagggca tggacgtggt cgacagcatc cgcaaggtca agaccggctc cagcggcttc
     3481 caccaggacg tgccgaagga agacgtgatc atcgagcgcg ccgaagtcgt ctgatcgtgc
     3541 ccgcgcgcga cccttcgcaa gtcgcctccg ccccggcccg gccgggcggg gccgccgcca
     3601 cgatgccgga agccgcccgc gcggcgcttc cggcattgtt catttccgac ctccacctga
     3661 ccgaggacga gcccgccaac gccgccgcct tcctcgactt cctgcacggc ccggcgcgcg
     3721 cggccgcgag cctcttcatc ctcggcgacc tgttcgaata ctgggccggc gacgacgacc
     3781 tcgagacccc cttcaacgcc cgcatcgccg cggcgatccg tcagctcgcc gacgccggca
     3841 ccgcggtctt cttcatgacc ggcaaccgcg acctgctcgc cggcccgacc ttcgccgcgg
     3901 cgatcggcgc gaccctgctc gaggacccgg cgcgcgtgcg ctgcggcgat cgcgacgagg
     3961 cagacgctgc cgacgccccc gtcctgctgc tcgcccacgg cgacgcgctg tgcaccgacg
     4021 acctcgccta ccaggccttc cgccgccagg tgcgcgaccc cgcctggcag gccggcttcc
     4081 tcgcccagcc gctggccgcg cgcaaggcct tcatcgccgg cctgcgccag aagagcgagg
     4141 ccgccaaggc cgaaaaagcc atggagatca tggacgtcaa cgccgacgcg gtcgccgcgc
     4201 tgctgcgcga acacggctac cccacgctgg tgcacggcca cacccaccgc cccgacaccc
     4261 accgcatccg ggtggacggc cacgactgca tccgccgcgt gctgcccgac tggcgcggcc
     4321 a
//
LOCUS       DAOQBS010000187         4314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_21874_length_4314_cov_15.947171, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000187 DAOQBS010000000
VERSION     DAOQBS010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21874_length_4314_cov_15.947171"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PKN25_14370"
     CDS             complement(<1..547)
                     /locus_tag="PKN25_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HNV92133.1"
                     /translation="MTQDFWTFCLSRLEQELPQQQFNTWIKTLRAEPGEGGWALIAPN
                     RFVLQWVRERYLRRVEELGEEFAGAPLAIELQLPPAGAARPARAAATADVAPARASAP
                     RSTIEATPAQAVETAETAPAVLDTVDAAEPAEPPVRPRRAAAPRSHSSEPSSGLDLAY
                     EKTRLNPDFTFDTLVTGRANDL"
     gene            866..1000
                     /gene="rpmH"
                     /locus_tag="PKN25_14375"
     CDS             866..1000
                     /gene="rpmH"
                     /locus_tag="PKN25_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNV92134.1"
                     /translation="MKRTYQPSVVRRKRTHGFLVRMKTRGGRAVIRARRAKGRHRLAV
                     "
     gene            1002..1364
                     /gene="rnpA"
                     /locus_tag="PKN25_14380"
     CDS             1002..1364
                     /gene="rnpA"
                     /locus_tag="PKN25_14380"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNV92135.1"
                     /translation="MVSPTGADQGFRSAHRLHKTDEYSSVFAFRRALRGRFYMLHYRP
                     NGLDTARLGVVVAKKLAKRANVRNLVKRIAREIFRRQRIELPAMDLVVRLNAPVAKAT
                     RAEINLDLLSLLQRLPRQ"
     gene            1361..1570
                     /gene="yidD"
                     /locus_tag="PKN25_14385"
     CDS             1361..1570
                     /gene="yidD"
                     /locus_tag="PKN25_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNV92136.1"
                     /translation="MKTVLIALLRFYRYAISPMLGRNCRFHPTCSEYAIEAVQRHGAL
                     RGGWMAAKRVGRCHPFNPGGYDPVP"
     gene            1591..>2255
                     /gene="yidC"
                     /locus_tag="PKN25_14390"
     CDS             1591..>2255
                     /gene="yidC"
                     /locus_tag="PKN25_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNV92137.1"
                     /translation="MDQRRLILFLIFSFSLVMLWDGWIKQNQPPVAQQQAAAGSAAPA
                     DGAVPTPTLSATPGQPVVPGEQAAQAAAAPRAVVETDLLRAEVSAQGGDIVRLELKQH
                     KASGEAGGNFVLLDDGTTRHQYAAQSGLIGEGLPTHKTLFQLPAGELRLKDGENGVEL
                     RLQAPEQNGVQVTKVMRFARGSYLIDVSYEIRNGSGQALAPHAYFQLTRDGNPAEQVE
                     AFGV"
     assembly_gap    2256..2355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2356..3000
                     /gene="yidC"
                     /locus_tag="PKN25_14395"
     CDS             <2356..3000
                     /gene="yidC"
                     /locus_tag="PKN25_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNV92138.1"
                     /translation="GWLTVIAAPLFWVLSWFHNLTGNWGWAIILVTVAIKAIFFPLSA
                     ASYKSMAKMRVLGPRLQRMKEMYGNDKAKMQQEMMNLYRTEKINPLGGCLPILVQIPV
                     FIALYWVLLGSVEMRHAPWLGWIQDLSAKDPYFILPIIMGVSMLVQMKLNPTPPDPIQ
                     AKVMMAMPIIFTFMFLWFPSGLVLYWVVNNCLSIAQQWQITRMIESGKSAGKPA"
     gene            2972..>4314
                     /gene="mnmE"
                     /locus_tag="PKN25_14400"
     CDS             2972..>4314
                     /gene="mnmE"
                     /locus_tag="PKN25_14400"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNV92139.1"
                     /translation="MESRPASPPDTIAAIATAPGRGGIGVVRVSGAGLGAFALALCGR
                     TPQPRIAHFTRFLDEHERPIDEGILLYFAAPASFTGEDVLELQGHGGPVVMQMLLERC
                     LQLGARLAEPGEFTRRAFLNGKLDLAQAEGVADLIEASTAAAARSAVRSLSGQFSEEV
                     HRIVDALIDLRMLVEATLDFPEEEIDFLERARAMPRLESIREALEGVLDRARQGALLR
                     SGLDVVLVGAPNVGKSSLLNQLAGEERAIVTDIAGTTRDALRETIQVEGIPLHIIDTA
                     GLRETEDRVERIGIERTWREIARADVILHLVEAGVAAEGDLDERLPQGVERIMVANKI
                     DLAGLPAGRVEAGGRVRLQVSARSGEGVELVRQELLRIAGWHAHGEDVILARERHLLA
                     LREALRHVEAAGTQLGALELFAEELRLAQEELGTITGEFSADDLLGVIFARFCIG"
BASE COUNT          643 a         1402 c         1462 g          707 t
ORIGIN      
        1 cgaggtcgtt ggcgcggccg gtgaccaggg tgtcgaaggt gaagtcgggg ttgagtcggg
       61 tcttctcgta ggcgaggtcg aggccggaac tcggttcgct gctgtgggag cggggtgctg
      121 cggcacgccg ggggcgcacg ggcggctccg ccggctcggc ggcgtccacc gtatcgagga
      181 ctgcaggcgc tgtttcggcg gtttcgaccg cctgggcggg cgtggcctcg atcgtcgacc
      241 tcggcgcgct cgcgcgcgcc ggagcgacat ccgccgtggc tgcggcgcgc gccgggcgtg
      301 cggcgccggc tggtggcagc tgcaactcga tggcgagcgg ggcgccggcg aactcctcgc
      361 cgagttcctc gacgcggcgc aggtagcgct cgcgcaccca ttgcagcacg aagcggttgg
      421 gcgcgatgag cgcccagcca ccctcgccgg gttcggcccg cagggtcttg atccaggtgt
      481 tgaactgctg ctggggcagc tcctgttcaa ggcgcgacag gcagaaggtc cagaaatctt
      541 gagtcacggc gggggcttgg atgtcgtcgg gcaggatggc gggaaagcgt gcggcgggtc
      601 aggctgcagc gggattcctg atgcgcggcg cctgggggcg cggccgggtt gctgtacgag
      661 tcggggagtt gtaccgacgg tgcactcgag cggcgcactg tgcgcgcctc gccccgcgca
      721 gcagcgcatg ggagcgtggc gtcgaaagac agcattctaa actgcggagg cagacttatc
      781 cacaggctcg ggcaaaaatt cttattgaca aacatggcat tatccattta actctcgcgt
      841 ttgacctaac actggcagtc cgatcatgaa acgcacttat cagccctccg ttgtccgccg
      901 caagcgcacc catggtttcc tggtccgcat gaagacccgt ggcggccgtg cggtcatccg
      961 cgcacgccgt gccaagggtc gtcaccgcct cgccgtctga ggtggtttcg cccacgggcg
     1021 ctgaccaggg tttccgcagc gctcacagat tgcacaaaac ggatgagtat tcatccgttt
     1081 ttgcttttcg gcgggcgctg cgcggccggt tctacatgct gcactaccgg cccaatggtc
     1141 tggacaccgc ccgcctcggc gtggtggtgg cgaagaagct ggccaagcgc gccaacgtgc
     1201 gcaacctggt caagcgcatc gcgcgcgaga tcttccggcg ccagcgcatc gagcttccgg
     1261 cgatggactt ggtcgtgcgc ctgaacgcgc cggtggccaa ggccacgcgc gccgagatca
     1321 acctcgacct gctctccctt ctgcaaaggc tgccccggca atgaagaccg tgctgatcgc
     1381 cctgctgcgt ttctaccgct acgcgatcag cccgatgctc ggccgcaatt gccgcttcca
     1441 cccgacctgc tccgagtacg cgatcgaggc cgtgcagcgc cacggtgcgt tgcgtggcgg
     1501 ctggatggcc gcgaaacggg tggggcgctg tcatcccttc aatccgggcg ggtatgatcc
     1561 cgttccctga cctgagacag aacgattccg atggatcaac gccgcctcat cctctttctg
     1621 attttctcct tctcgctcgt catgctgtgg gacggctgga tcaagcagaa ccagcctccc
     1681 gtggcccagc agcaggccgc cgccggaagc gcggcgccgg ccgacggcgc ggtgcccacg
     1741 cccacgttga gcgccacgcc cggccagccg gtggtgccgg gcgagcaggc cgcccaggct
     1801 gcggccgcgc cgcgtgccgt ggtcgagacc gatctgctgc gtgccgaggt ctcggcgcag
     1861 ggcggtgaca tcgttcgcct cgagctcaag cagcacaagg cgagcggcga ggcgggcggc
     1921 aacttcgtgc tgctcgacga cggcaccacg cggcaccagt acgccgcgca gtcgggcctg
     1981 atcggcgagg gcctgcccac ccacaagacc ctgttccagc tgcccgctgg cgagctgcgc
     2041 ctgaaggacg gcgagaacgg cgtggagctg cgtctgcagg cgcccgagca gaacggcgtg
     2101 caggtcacca aggtgatgcg cttcgcgcgc ggcagctacc tgatcgacgt gtcctacgag
     2161 atccgcaacg gatcgggcca ggcgctcgcg ccgcacgcct acttccagct cacccgcgac
     2221 ggcaacccgg ccgagcaggt cgaggccttc ggcgtnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnggctg gctgaccgtg atcgcggcgc cgctgttctg ggtgctgtcc
     2401 tggttccaca acctgactgg caactggggc tgggcgatca tcctggtcac cgtcgcgatc
     2461 aaggcgatct tcttcccgct gtcggcggcg agctacaagt cgatggccaa gatgcgcgtg
     2521 ctcggccccc gcctgcagcg catgaaggag atgtacggca acgacaaggc caagatgcag
     2581 caggagatga tgaatctcta ccgcaccgag aagatcaacc cgctcggcgg ctgcctgccg
     2641 atcctggtgc agatcccggt gttcatcgcc ctgtactggg tgctgctcgg cagcgtcgag
     2701 atgcgccacg cgccgtggct gggctggatc caggacctct cggccaagga cccctacttc
     2761 atcctgccga tcatcatggg cgtgtccatg ctcgtgcaga tgaagctcaa cccgacgccg
     2821 ccggatccga tccaggccaa ggtcatgatg gcgatgccga tcatcttcac cttcatgttc
     2881 ctgtggttcc cgtccggcct ggtgctgtac tgggtggtga acaactgcct gtcgatcgcc
     2941 cagcagtggc agatcacgcg gatgatcgaa agtggaaagt cggccggcaa gcccgcctga
     3001 cacgattgcc gcgatcgcga cggcaccggg ccgcggcggc atcggtgtcg tccgcgtctc
     3061 gggtgccggt ctcggcgcct tcgcgctcgc gctgtgcggc cgtacgccgc agccgcgcat
     3121 cgcgcacttc acccgcttcc tcgacgagca cgagcggccg atcgacgagg ggatcctgct
     3181 ctacttcgcc gcgccggcgt ccttcacggg cgaggacgtg ctcgagctgc agggccacgg
     3241 cggcccggtg gtcatgcaga tgctgctcga gcgctgcctg caactcggcg cccgcctggc
     3301 cgagcccggc gaattcaccc gtcgcgcctt tctcaacggc aagctcgatc tcgctcaggc
     3361 cgagggtgtc gccgacctga tcgaggcctc cactgcggcg gctgcgcgct cggcggtgcg
     3421 ctcgctgtcc ggacagttct ccgaggaagt ccatcgcatc gtcgacgcgc tcatcgacct
     3481 gcgcatgctg gtcgaggcaa cgctggattt tcccgaggaa gagatcgact tcctcgagcg
     3541 tgcgcgcgcg atgccgcggc tcgaatccat ccgtgaggcg ctcgagggcg tgctcgaccg
     3601 tgcgcgccag ggggcgctgc tgcgcagcgg gctcgacgtg gttctcgtgg gtgcgcccaa
     3661 cgtcggcaag tccagcctgc tcaaccagct cgccggcgag gagcgcgcga tcgtcaccga
     3721 catcgccgga acgactcgcg acgcgctgcg cgagacgatc caggtcgagg gtatcccgct
     3781 ccacatcatc gacaccgccg gcctgcgcga gaccgaggac cgggtcgagc gcatcggcat
     3841 cgagcgcacc tggcgcgaga tcgcgcgcgc cgacgtgatc ctgcatctgg tcgaggcggg
     3901 cgtggcggcc gagggcgacc tcgacgagcg cctgccgcaa ggtgtagagc gcatcatggt
     3961 ggcgaacaag atcgacctcg caggattgcc cgcgggccgc gtggaggcgg gcggacgggt
     4021 tcgcctgcag gtgtcggcgc gaagcgggga gggtgtcgag ctggtgcgcc aggagctgtt
     4081 gcggatcgcc ggctggcatg cgcacggcga ggatgtgatt cttgcgcgcg agcgtcatct
     4141 gctcgcgctg cgcgaggcgc tccgccatgt ggaggctgca ggcacgcaac tcggtgcgct
     4201 cgaactcttt gccgaggagc tgcgcctcgc gcaggaggaa ctcggcacga tcaccggcga
     4261 gttcagcgcc gacgacctgc tcggggtgat cttcgcccgc ttctgcatcg gaaa
//
LOCUS       DAOQBS010000188         4290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_22061_length_4290_cov_13.163164, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000188 DAOQBS010000000
VERSION     DAOQBS010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22061_length_4290_cov_13.163164"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      228..314
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            521..1612
                     /gene="gcvT"
                     /locus_tag="PKN25_14405"
     CDS             521..1612
                     /gene="gcvT"
                     /locus_tag="PKN25_14405"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNV92140.1"
                     /translation="MTIAAKQTPLHAAHVAAGARMVDFAGWDMPVNYGSQIEEHHAVR
                     RDAGMFDVSHMLALDLAGPDATTWLRGLLANDVAKLKDNGKALYSCMLNERGGVIDDL
                     IVYRFSDADYRIVVNAGTADKDVAWMRQRIAATGANVTLQARRDLAMIAVQGPRAIER
                     ATLALPELSTANGVPAPFFGARVGDLLIARTGYTGEDGLEIAVPASRAEAMWNALAAA
                     GVRPCGLGARDTLRLEAGMNLYGQDMDDHVSPLNAGLSWTVDMKDEARDFLGRAALQA
                     NPASQRLLGLILEDKGVLRSHMKVFTSGGEGETTSGSFSPTLEKSIAFARVPLATQPG
                     EAVEVDIRGKRLKARTVKLPFVRNGKALV"
     gene            1734..2120
                     /gene="gcvH"
                     /locus_tag="PKN25_14410"
     CDS             1734..2120
                     /gene="gcvH"
                     /locus_tag="PKN25_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNV92141.1"
                     /translation="MSNVPSTLKYTKSHEWIRVEDDGTLTIGVTDHAQEALGDIVFLE
                     LPEAGRALSAGETCAVIESVKAASDIYAPVAGEVIANNQDAIDAPESVNADAYSAWLF
                     KLAPANAADVAALMDAAAYEAEIAQA"
     gene            2123..>4290
                     /gene="gcvP"
                     /locus_tag="PKN25_14415"
     CDS             2123..>4290
                     /gene="gcvP"
                     /locus_tag="PKN25_14415"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNV92142.1"
                     /translation="MTDTLLSAPLARLEQRDAFVHRHLGPDAGEIGRMCASIGVADVD
                     ALIAQTVPAGIRLASPLPLAGAMPEHEALARLKAIAGRNVVKKSLIGQGYYGTHVPAV
                     VLRNVLENPGWYTAYTPYQAEISQGRLEALLNYQQMVIDLTGLELANASLLDEATAAA
                     EAMTMARRVSKSKSNVFFIDEACFAQTIDVVRTRAHYFGYELVYGKAAEAGSHDCFGA
                     LLQYPNARGEVVDLTAPIAELKAKGAVVAVASDLMALVLLKSPGAMGADIALGSAQRF
                     GVPMGFGGPHAAFFATRESYVRSMPGRIIGVSKDARGKTALRMTLQTREQHIRREKAN
                     SNICTSQVLLANLSGFYAVYHGPQGLRTIAARIHRLAAVLAAGLREAGFGLHDRAFFD
                     TVEVEVGARAPTILRAAEEAGFNLRSVSGTTLGLSVDETTTREDIATILGCFGASTDV
                     SAIDARVAAAGGALPAELLRSDAVLAHPVFNTHHTEHEMLRYLKKLQNRDLALDHSMI
                     SLGSCTMKLNATSEMIPITWPEFANLHPFAPREQAAGYLEMIEGLADYLRAVTGFPAI
                     SMQPNSGAQGEYAGLVAIARYHASRGEANRKICLIPKSAHGTNPATAQMCGMQVVVVE
                     CDDNGNVDVADLKAKAEKHAADLAALMITYPSTHGVFEESIREICTIVHQYGGQVYMD
                     GANLNAQVGLTSPATIGADVSHMNLHKTFCIPHGGGGPGMG"
BASE COUNT          747 a         1611 c         1320 g          612 t
ORIGIN      
        1 aagaaatccc cgcccccctt gcatccgaca accgcgaacg gccgatcgga tcggcaccac
       61 gctccaatgg caattgcgct cctaagcgcg acttgcgtcg tacctgcatc gcgagagttg
      121 ctttgcagcc cccggaggtc gatgccaggc gctctaagct tcgcgacgat ggcgcgcgcg
      181 gacgcatccg tcgcggttgc ggcaccgtgg ggcaagtgat atcgtccgcg cgacacgcgg
      241 gagagaactc ctcgatggag tcgccgaagg cgcaaccccc ggaaccgctc aggcaaaagg
      301 accgcgtgcg cagcaaaagt ctggagagcg gtacgggcgc aaggcgccgg cgtatccacc
      361 gaaggggcac accgtcgtca gccgtaacat cgcatacata tatgtctgca gatgccacgc
      421 gatcacggga aaatctctca ggtaaaagga cagatggggc gtgctcggtg acaaaccgta
      481 cccgcccctt tctcgttcac cctgacggag cgccttcgcg atgactatcg ctgccaaaca
      541 gacccccctc cacgccgccc acgtcgccgc cggcgcacgc atggtcgact tcgccggctg
      601 ggacatgccg gtgaactacg gttcgcagat cgaggagcat cacgccgtgc gccgcgacgc
      661 cggcatgttc gacgtctcgc acatgctcgc cctcgacctc gccggccccg acgcaaccac
      721 ctggctgcgc ggtctgctcg ccaacgacgt cgccaagctc aaggataacg gcaaggcgct
      781 ctactcctgc atgctcaacg agcgcggcgg cgtgatcgac gacctgatcg tctaccgctt
      841 cagcgatgcc gactaccgca tcgtcgtaaa cgccggcacc gccgacaagg acgtcgcctg
      901 gatgcgccag cgcatcgccg cgaccggtgc caacgtgacc ctgcaggcgc gccgcgacct
      961 ggcgatgatt gccgtccagg gtccgcgcgc gatcgagcgc gccaccttgg ccctgccgga
     1021 gctctccacc gccaacggcg tgcccgcccc cttctttggc gctcgcgtgg gcgacctgct
     1081 gatcgcacgt accggctaca ccggcgagga cggactggag atcgccgtcc ccgcgagccg
     1141 cgccgaggcg atgtggaatg cgctcgccgc cgccggtgtg cgtccctgcg gcctgggcgc
     1201 gcgcgacacc ctacggctgg aagccggcat gaacctctac ggccaagaca tggacgacca
     1261 cgtttccccc ctgaatgccg gcctgtcgtg gacggtggac atgaaggacg aggcgcgcga
     1321 cttcctcggt cgcgccgcgc tgcaggccaa ccctgccagt cagcgcctgc ttggcctcat
     1381 cctcgaggac aagggcgtgc tgcgctcgca catgaaggtc ttcacctccg gcggcgaagg
     1441 cgagaccacc agcggaagct tctcgcccac gctggaaaag tcgatcgcct tcgcacgcgt
     1501 ccctctcgcg acgcagccgg gcgaagccgt cgaggtggac attcgcggca agcgcctgaa
     1561 ggcacgcacc gtcaagctgc ccttcgtacg taacggcaag gccctcgtct gacccgatgt
     1621 ccgagggcgg ccatcgagcc gcccttcatg tcgcagccgc acatcaccga cgagccggac
     1681 gcaacactgc atttcttcac ccgcacaccc catcttcccg accggagagc accatgagca
     1741 acgttcccag caccctcaag tacaccaagt cccacgaatg gatccgcgtc gaggacgacg
     1801 gcaccctgac catcggcgtc accgaccacg cccaggaggc cctcggcgac atcgtcttcc
     1861 tcgaactgcc ggaagccggc cgtgcgctgt ccgccggcga aacctgcgcg gtgatcgaat
     1921 cggtcaaggc tgcctccgac atctacgccc ccgtggcagg tgaagtgatc gccaacaatc
     1981 aggacgcgat cgacgccccc gagagcgtca acgccgacgc gtactccgcc tggctgttca
     2041 agctcgcgcc ggccaacgcc gccgacgtcg ccgcgctgat ggacgccgca gcctacgaag
     2101 cggaaatcgc ccaggcctga ccatgaccga caccctgctc tccgccccgc tcgcccgcct
     2161 cgagcagcgc gacgccttcg tccaccgcca cctcggcccc gatgcgggcg agatcgggcg
     2221 catgtgcgcg agcatcggcg tcgccgacgt cgacgccctg atcgcgcaga ccgtgcccgc
     2281 cggcatccgc ctcgcgtcgc cgctgccgct cgccggcgcg atgcccgagc acgaggcgct
     2341 tgcccgcctg aaggccatcg ccggccgcaa cgtggtgaag aagtccctga tcggtcaggg
     2401 ctactacggc acccacgtcc ccgcggtcgt gctgcgcaac gtgctcgaga acccgggctg
     2461 gtacaccgcc tacacgccct accaggccga gatctcccaa ggccgcctcg aagccctgct
     2521 gaactaccag cagatggtga tcgacctcac cggcctggag ctcgccaacg cctcgctgct
     2581 cgacgaggcc accgccgccg ccgaggcgat gacgatggcc cggcgcgtat cgaagtcgaa
     2641 gtcgaacgtg ttcttcatcg acgaggcctg cttcgcgcag accatcgacg tggtgcgcac
     2701 ccgtgcgcac tacttcggct acgagctggt ctacggcaag gctgccgagg ctggcagcca
     2761 cgactgcttc ggcgcgctgc tgcagtaccc gaacgcacgc ggcgaggtcg tcgacctcac
     2821 ggccccgatc gcagaactca aggccaaggg cgccgtggtc gcggtcgcca gcgatctgat
     2881 ggccctggtg ctgctcaaga gccccggcgc catgggcgcc gacatcgcgc tcggttcggc
     2941 gcagcgcttc ggcgtgccga tgggcttcgg cggcccgcat gccgccttct tcgcgacccg
     3001 cgaaagctac gtgcgctcaa tgcccggccg tatcatcggc gtctccaagg acgcgcgtgg
     3061 caagaccgcg ctgcgcatga cgctgcagac ccgtgagcag cacatccgcc gcgaaaaggc
     3121 gaactccaac atctgcacct cgcaggtgct gctggccaac ctctccggct tctacgccgt
     3181 ctatcacggc ccgcagggcc tgcgcaccat cgccgcgcgc atccaccgcc tcgccgcggt
     3241 gctcgccgcc ggcctgcgcg aggccggctt cggcctgcac gaccgtgcct tcttcgacac
     3301 cgtcgaggtc gaggtcggcg cgcgcgcgcc caccatcctg cgcgcggccg aggaagccgg
     3361 cttcaacctg cgcagcgtct ccggcaccac gctcggtctg tcggtggatg agaccaccac
     3421 ccgcgaggac atcgccacga tcctcggctg cttcggcgcc tccaccgacg tcagcgcgat
     3481 cgacgcccgc gtcgcggccg caggcggcgc cctgccggcg gaactgctgc gcagcgacgc
     3541 cgtgctcgcg cacccggtgt tcaacacgca ccacaccgag cacgagatgc tgcgctacct
     3601 caagaagctg cagaaccgcg acctcgcgct cgaccactcg atgatctcgc tcggctcgtg
     3661 cacgatgaag ctgaacgcca ccagcgagat gatcccgatc acctggccgg agttcgccaa
     3721 cctgcacccg ttcgcgccgc gcgagcaggc cgccggctac ctcgagatga tcgagggcct
     3781 ggccgactac ctgcgcgcgg tcaccggctt cccggcgatc agcatgcagc cgaactcggg
     3841 tgcgcagggc gagtacgccg gcctggtggc gatcgcgcgc taccacgcca gccgcggcga
     3901 ggccaaccgc aagatctgcc tgatcccgaa gtccgcccac ggcaccaacc cggcgaccgc
     3961 gcagatgtgc ggcatgcagg tggtggtggt cgagtgcgac gacaacggca acgtcgacgt
     4021 cgccgacctc aaggccaagg cggagaagca cgccgccgac ctggccgcac tgatgatcac
     4081 ctacccgtcc acccacggcg tgttcgagga gtcgatccgc gagatctgca ccatcgtcca
     4141 ccagtacgga ggtcaggtgt acatggacgg cgccaacctc aacgcgcagg ttggcctgac
     4201 ctcgccggcg acgatcggcg ccgacgtgtc gcacatgaac ctgcacaaga ccttctgcat
     4261 cccgcacggc ggcggcggtc cgggcatggg
//
LOCUS       DAOQBS010000189         4289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_22069_length_4289_cov_12.781294, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000189 DAOQBS010000000
VERSION     DAOQBS010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22069_length_4289_cov_12.781294"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKN25_14420"
     CDS             complement(<1..262)
                     /locus_tag="PKN25_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV92143.1"
                     /translation="MRYIELNPVRAGMVDHPGAYRWSSYRCNAQGEADELIRPHAVFT
                     ALDADPRICGERHRGLFLNALEPGDVGRKRAGTNDSAALGDDR"
     gene            436..684
                     /locus_tag="PKN25_14425"
     CDS             436..684
                     /locus_tag="PKN25_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNV92144.1"
                     /translation="MSEQREPHVAQKAPYAVEVEAGKKYFWCACGLSKKQPFCDGSHK
                     GSGFAPTAFTPEKSGTVHLCGCKHTANAPYCDGTHTKL"
     gene            695..1258
                     /locus_tag="PKN25_14430"
     CDS             695..1258
                     /locus_tag="PKN25_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNV92145.1"
                     /translation="MRFLLACLGLLLPIAAACAETDEAALWAALREGGHVALMRHAIA
                     PGVGDPAGFRLDDCTTQRKLSAQGRAQARAIGERFRANGIATAAVFSSQWCRCLDTAR
                     ELALGEVVAFPGLNSFFADRGEEARHTAAVRALIGERARSPLPLVLVTHQVNITALTG
                     VFPASGEIIVLRVDGDTPSVAGRIRTD"
     gene            complement(1319..1891)
                     /locus_tag="PKN25_14435"
     CDS             complement(1319..1891)
                     /locus_tag="PKN25_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNV92146.1"
                     /translation="MSLPQPKIPFGPDDYLAWEQDQPIRSEYVDGEVFAMSGASDAHG
                     TAAGNLFAGLHAHLRGTPCKPFIADMKVHVEAANSFFYPDILVTCDPRDRSAEASHVK
                     RHPLLIVELLSPSTEAYDRGNKFAAYRTLAALQEYVLVSLEERRIEVFRRDDSGHWVL
                     YPFALDEQLELASVGLRCPVAEVFEGVEGE"
     gene            complement(2005..2562)
                     /locus_tag="PKN25_14440"
     CDS             complement(2005..2562)
                     /locus_tag="PKN25_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV92147.1"
                     /translation="MFLLDWLFGKHQAKAEAAPSSATVPAPEPAHSMEDSESFAPGTT
                     IRYSPELVERLVRDHRLMLERFGAIREAAHAGRFDEVVPLLERFRVDLHAHLLVENVR
                     LYVYLEHTLADDPVSHALMHEFRHDMDEIGKKVIAFLARYVRLGEDPALAASFAADLE
                     RVGAVLVDRIQREEDTLYPLYAPVS"
     assembly_gap    2684..2783
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2784..3104
                     /gene="cutA"
                     /locus_tag="PKN25_14445"
     CDS             <2784..3104
                     /gene="cutA"
                     /locus_tag="PKN25_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNV92148.1"
                     /translation="RALLVFTNLPDAASAEALANALIDRQLAACVNMLAPCTSVYRWQ
                     GATETATEVPLLIKTTPARYAALEAAIRAHHPYELPEIVAVPVTQGLPGYLDWVAAGT
                     RDDD"
     gene            3198..>4289
                     /gene="dsbD"
                     /locus_tag="PKN25_14450"
     CDS             3198..>4289
                     /gene="dsbD"
                     /locus_tag="PKN25_14450"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNV92149.1"
                     /translation="MHRLFLLLALIASLLLPAAARANPIEPEKAFAMRAQALDAQTIE
                     VVFEIAKDYYLYGDKFRFEAEPAGVGFGAMEKPAGKKHKDDFFGEVETHRGELRILVP
                     VQAPAGTTRFELFATSQGCWDGGICYPPTTQQASIDLAAPPKKAGGFLDSVLGGRAGT
                     GSAASPTPVALAAPEDGSGGGAVSSDESGDIARLLSGASVPIILLSFFGFGLLLAFTP
                     CTFPMIPILSGIIVGQGNKVSHTRAFVLSLAYVLGMAVTYALAGVAAGMTGTMLSAAL
                     QNVWVLSTFALMFVLLSLSMFGFYELQLPSTLQSKLADTASHGKGGNLGGVTLMGVLS
                     ALIVGPCVAAPLAGALLYIAQTGDAVLGGW"
BASE COUNT          642 a         1451 c         1451 g          645 t
ORIGIN      
        1 agcggtcgtc gccgagcgcg gcgctgtcgt tggtccccgc gcgcttccgc cccacgtcgc
       61 cgggctccag ggcgttcaga aagagcccgc ggtgacgctc gccacaaatc cgggggtcgg
      121 catcgagggc agtgaagacg gcatgcggcc ggatcagctc gtccgcttca ccctgcgcgt
      181 tgcagcggta gctcgaccag cggtaggcgc ccgggtgatc caccataccg gcccgcaccg
      241 ggttgagttc gatgtagcgc atgcaactca agagatggag cacgtgctgc acgaggcacg
      301 agcgataccg cccctcccac agcgtgccgc tgcgcgcgta ggcatgattg acacgccctg
      361 cggtccggcg caaggtttct cagaggctgc gccgcggccg accggcaaga ccacaaactc
      421 aaaggagaca aggcgatgtc cgaacaacgc gaaccccatg tcgcgcagaa agcgccctat
      481 gccgtcgagg tcgaggcggg caagaagtat ttctggtgcg cctgtggcct gagcaagaag
      541 cagccgttct gcgatgggtc ccacaagggc agcgggtttg ctcccaccgc cttcaccccc
      601 gagaaatcgg gcacggtcca tctgtgcggc tgcaagcaca ccgccaacgc gccgtattgc
      661 gacggaacgc acacgaagct ttgaccgggc ggccatgcgc ttcctgcttg cctgcctggg
      721 cctgttgctg ccgattgccg ccgcctgcgc cgagacggac gaggcagcct tgtgggcggc
      781 cttgcgcgag ggcgggcatg tcgcgctgat gcgccatgcg atcgcacccg gcgtcggtga
      841 cccggcgggc tttcggctgg acgactgcac cacccagcgc aagctgtcgg cgcagggtcg
      901 ggcgcaggcg cgcgcgatcg gcgagcgctt ccgcgccaac ggcatcgcca ccgcggcggt
      961 gttctccagc cagtggtgtc gctgcctcga tacggcgcgc gaactggcgc tgggcgaggt
     1021 ggtggcgttc ccggggctga actccttctt cgccgaccgc ggcgaggagg cgcggcacac
     1081 cgctgcggtc cgcgcgctga tcggggaacg cgcgcgcagt cccttgccgc tggtgctggt
     1141 gacgcatcag gtgaacatca ccgcgctgac gggggtgttt ccggcctcgg gcgagatcat
     1201 cgtgctgcgg gtcgacggcg acacgccgag cgtggccggc cgcatccgga ccgattgaga
     1261 cagccggctc gccttttcct gccgtgcatc gcggggacgg cgaaaacgcc cacccgcgct
     1321 actcgccctc caccccttcg aagacctcgg ccaccgggca gcgcaggccg acgctcgcga
     1381 gttccagttg ctcgtcgagg gcgaaggggt agagcaccca gtgcccactg tcgtcgcgcc
     1441 ggaagacctc gatacgacgc tcttcgagcg agacgagtac atattcctgc agcgcagcca
     1501 gcgtgcggta ggcggcgaac ttgttgccgc ggtcgtaggc ctcggtggac ggcgagagca
     1561 gctcgacgat cagcagcggg tgacgcttga cgtggctggc ctcggcgctg cggtcgcgcg
     1621 ggtcgcaggt gacgaggatg tcggggtaga agaagctgtt ggccgcctcg acgtggacct
     1681 tcatgtcggc gatgaagggc ttgcaggggg taccgcgcag atgcgcatgc aggccggcga
     1741 aaaggttgcc ggctgccgta ccgtgcgcgt ccgacgcgcc gctcatggcg aagacctcgc
     1801 catcgacgta ttcgctgcgg atgggctgat cctgttccca ggccaggtag tcgtcggggc
     1861 cgaacgggat cttgggctgg ggcaggctca tcgggcgtcc tcgctgcaat tcgttgcggt
     1921 ttgcgcttcg agcatagcgg cgagggacgg gacaggcaat cgagaggccc gtcttccggg
     1981 tcgtcggacg acgagtgcag cggctcagct caccggcgca tagagcggat acaaggtgtc
     2041 ctcctcgcgc tggatgcgat cgaccagcac ggctccgacg cgctcgaggt cggcggcgaa
     2101 gctcgcggcg agcgccgggt cttcgccgag gcggacgtag cgggcgagga aggcgatcac
     2161 cttcttgccg atctcgtcca tgtcgtggcg gaactcgtgc atcagcgcgt ggctcaccgg
     2221 atcgtcggcg agggtgtgct cgagatacac gtacaggcgc acgttctcga ccagcagatg
     2281 ggcgtgcagg tcgacgcgga agcgctcgag caggggcacg acctcgtcga agcgccccgc
     2341 atgcgcggcc tcacggatgg cgccgaagcg ttcgagcatc agacgatgat cgcgcaccag
     2401 gcgctcgacc agttcggggc tgtagcggat ggtggtgccg ggagcgaagc tctcggagtc
     2461 ctccatggaa tgcgcgggtt cgggggcagg aaccgtggcg gacgagggcg cggcctccgc
     2521 cttggcctgg tgcttgccga acagccagtc gagcaggaac atgatgtgct ccggatggag
     2581 ccgcggaggg cggccgatgc gtgaaggatt ccgacgctaa tacaggtgca tgatggactt
     2641 ccatggcaca gatcaaatcc gcgccggatc catccgcgac accnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnncgcgccc tgctcgtctt cacgaacctg cccgacgccg
     2821 ccagcgccga ggccctggcg aacgcgctga tcgaccggca gctcgccgcc tgcgtgaaca
     2881 tgctcgcgcc ctgcacctcg gtgtatcgct ggcagggcgc gaccgagacc gcgaccgagg
     2941 taccgctgct gatcaagacc acgccggcgc gctacgccgc gctggaggcg gcgatccggg
     3001 cccaccaccc ctacgaactt ccggagatcg tcgcggtccc tgtgacgcag ggcttgcccg
     3061 gctatctcga ctgggtcgcc gccggaacga gggacgacga ctgatcgccc gccggcgcgt
     3121 ccccggcacc gccccgcccg atcgacccgt ccgcgggagc cgcagtgcgc ccgcccaccg
     3181 ccgcctagaa gaccacgatg caccgccttt tcctcctgct cgccctgatc gcgagcctgc
     3241 tcctgcccgc cgccgcgcgc gccaacccga tcgagcccga gaaggccttc gcgatgcgcg
     3301 cgcaggcgct cgacgcgcag accatcgagg tggtgttcga gatcgccaag gactactacc
     3361 tctacggcga caagttccgc ttcgaggccg agcccgccgg cgtgggcttc ggcgcgatgg
     3421 aaaagcccgc gggcaagaag cacaaggacg acttcttcgg cgaggtcgag acccaccgcg
     3481 gcgagctgcg catcctggtc ccggtacagg cgcccgccgg caccacccgc ttcgagctct
     3541 tcgccaccag ccagggctgc tgggatggcg gcatctgcta tccgcccacc acgcagcagg
     3601 cctcgatcga cctcgccgcg ccgccgaaga aggcgggcgg cttcctcgac agcgtgctcg
     3661 gcggccgcgc gggcaccggc agcgccgctt cgcccacccc ggtcgcgctt gccgcgccgg
     3721 aggacggcag cggcggcggc gccgtctcca gcgacgagag cggcgacatc gcccgcctgc
     3781 tttccggcgc cagcgtgccg atcatcctgc tgagcttctt cggcttcggc ctgctgctcg
     3841 ccttcacgcc ctgcaccttc ccgatgatcc cgatcctgtc gggcatcatc gtcggccagg
     3901 gcaacaaggt ctcgcacacg cgcgccttcg tgctctcgct cgcctacgtg ctgggcatgg
     3961 cggtgaccta cgcgctcgcc ggggtcgccg ccggcatgac cggcaccatg ctgtcggcgg
     4021 cgctgcagaa cgtgtgggtg ctgtcgacct tcgcgctgat gttcgtgctg ctgtcgctgt
     4081 cgatgttcgg cttctacgag ctgcagctgc ccagcacgct gcagagcaag ctggccgaca
     4141 ccgccagcca cggcaagggc ggcaacctcg gcggcgtcac cctgatgggc gtgctgtcgg
     4201 cgctgatcgt cggcccctgc gtggcggccc ccctcgccgg cgccctgctc tacatcgcgc
     4261 agaccggcga tgccgtgctc ggcggctgg
//
LOCUS       DAOQBS010000190         4278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_22146_length_4278_cov_15.313521, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000190 DAOQBS010000000
VERSION     DAOQBS010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22146_length_4278_cov_15.313521"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1947)
                     /gene="htpG"
                     /locus_tag="PKN25_14455"
     CDS             complement(4..1947)
                     /gene="htpG"
                     /locus_tag="PKN25_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNV92150.1"
                     /translation="MSEVHNPSQPSSQTLAFQAEVKQLLHLMIHSLYSNREIFLRELV
                     SNASDACDKLRFEALDNGALYEDDSELKIRIGFDAEARTITIADNGIGMSRDEAIAHL
                     GTIARSGTKEFFGKLTGDQKKDAHLIGQFGVGFYSAFIVADKVTVVSRRAGLPADQGV
                     KWECSMTGDEAGAYTVAQVDKPGRGTEITLHLREGQEDLLSSWKLKSLIRKYSDHIVQ
                     PIVMKKEEWNEEQKKQVVTDEDETVNQANALWTRSKNDITDDEYTAFYKHVAHDFEDP
                     LAWTHSRVEGRHEYTNLLYIPKNAPFDLWDRNARHGIKLYVKRVFIMDDAEKLMPTYL
                     RFVRGVVDSADLPLNVSREILQESKDIDTIRAGCTKKVLTLLESLANSDEAADKDKYA
                     EFWKAFGKVLKEGVGEDFANKDKIAGLLRFASTKLDTPDEVVSLADYIGRMKEGQDKI
                     YFVTAETFNAAKNSPHLEVFRKKGIEVLLLTDRVDEWVTGNLTEFDGKAMVSVAKGGL
                     DLGALEDEAEKQEVEKAADEYKELVDKMKASLGERVKDVKVTLRLTDSPACLVADEHD
                     LGMNLARILKAAGQNAPVSKPILEINPNHPAVLRLKYEDKHFDDWAAVLFDQALLAEG
                     GTLDDPATFVKRINQLMMAMSGN"
     gene            complement(2034..3668)
                     /locus_tag="PKN25_14460"
     CDS             complement(2034..3668)
                     /locus_tag="PKN25_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV92151.1"
                     /translation="MRFESLTVARRLALMLLVALAGMVLLALAALYENRDDMREGYAR
                     NVRSQIELATGVLAHFHALEQAGVLDREAAQKAAKDTLRGLRYQNNEYFFVLDLELNM
                     LMHPLRPALEGKLADLKDSAGKHFVREMVTLARAQGQGFVDYTWPRSGVATDPAQPKL
                     SYVKAFPSWGWVVGSGVYVDDIDAAFNDAALRFGALVAVVLVLLGLIGVWVIRGVTGA
                     LGGEPAYAGAIMKRAAAGDLAVDVEQRGRGDSLLGNLSNMLAQLRAMIGAIGGSASRL
                     GASAREIHAVSRSVSEASVSQTGATASIAAAIEEMTVSIEQISDSARLAEQHSSTAAG
                     LADEGAGKAERAAREMQAIAARVGDAAQKIQQLVARADEVGTIANVIKEIAGQTNLLA
                     LNAAIEAARAGEQGRGFAVVADEVRGLAERTAVATVQIEKVIEGIQNETRATVGAMAE
                     VSSQVDGGVALVGDATDSLHRIRSTAGEALERIREVAYATGEQSSASTEIARQVQTIT
                     EMADGTSGSMHSVVVAAEQLQGLAAELDALVGRFRC"
     gene            complement(3799..>4278)
                     /gene="greB"
                     /locus_tag="PKN25_14465"
     CDS             complement(3799..>4278)
                     /gene="greB"
                     /locus_tag="PKN25_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HNV92152.1"
                     /translation="PGSKNYMTRRGYDALRVELDQLVRVERPRLVETVAWAAGNGDRS
                     ENGDYIYGKKRLREIDRRIRFLIKRIESAEVVDPERQQGLEQVFFGATVSFSDLDTAE
                     EQTLQIVGVDEADASQGRISWISPLARAVLKARVGDVVRFHSPAGVREIEIGAVEYR"
BASE COUNT          606 a         1475 c         1427 g          770 t
ORIGIN      
        1 ggatcagttc ccgctcatcg ccatcatcag ctggttgatg cgcttgacga aggtggccgg
       61 atcgtcgagc gtgccgccct cggccagcag ggcctggtcg aacagcaccg ccgcccagtc
      121 gtcgaagtgc ttgtcctcgt acttcaggcg cagcacagcc gggtggttgg ggttgatctc
      181 gaggatgggc ttggagaccg gcgcgttctg gcccgcagcc ttgaggatgc gcgcgaggtt
      241 catgccgagg tcgtgctcgt cggcgaccag gcaggccggg gagtcggtca ggcgcagcgt
      301 caccttcacg tccttgacgc gctcgcccag gctcgccttc atcttgtcga ccagctcctt
      361 gtactcgtcg gcggccttct cgacctcctg cttctcggcc tcgtcctcca gcgcgcccag
      421 gtcgagcccg cccttggcga ccgacaccat cgccttgccg tcgaactcgg tgaggttgcc
      481 ggtcacccac tcgtcgacgc ggtcggtgag cagcagcacc tcgatgccct tcttgcggaa
      541 gacctcgaga tgcgggctgt tcttcgccgc gttgaaggtc tcggcggtga cgaagtagat
      601 cttgtcctgg ccttccttca tgcggccgat gtagtcggcg agcgacacca cctcgtcggg
      661 cgtatccagc ttggtcgagg cgaagcgcag caggccggcg atcttgtcct tgttggcgaa
      721 gtcctcgccc acgccttcct tgagcacctt gccgaaggcc ttccagaact cggcgtactt
      781 gtccttgtcc gcggcctcgt cgctgttggc caggctctcg agcagggtca gcaccttctt
      841 ggtgcagccg gcgcggatgg tgtcgatgtc cttggattcc tgcaggatct cgcgcgacac
      901 gttgagcggc aggtcggccg aatcgaccac cccgcgcacg aagcgcaggt aggtcggcat
      961 cagcttctcg gcgtcatcca tgatgaagac gcgcttcaca tagagcttga tgccgtgcct
     1021 ggcgttgcgg tcccacaggt cgaagggcgc gttcttcggt atgtagagca ggttcgtgta
     1081 ttcgtggcga ccctcgacgc gcgagtgcgt ccacgccagc gggtcctcga agtcgtgggc
     1141 gacgtgcttg tagaaggcgg tgtactcgtc gtccgtgatg tcgttcttcg agcgcgtcca
     1201 cagcgcgttg gcctggttga cggtctcgtc ctcgtcggtc acgacctgct tcttctgctc
     1261 ctcgttccat tcctccttct tcatcacgat cggctgcacg atgtgatcgg agtacttgcg
     1321 gatcaggctc ttcagcttcc acgacgacag caggtcttcc tggccctcgc gcaggtgcag
     1381 cgtgatctcc gtgccgcgcc cgggcttgtc gacctgcgcc acggtgtagg cgccggcttc
     1441 gtcgcccgtc atcgagcatt cccacttcac gccctggtcg gcagggagcc cggcgcggcg
     1501 cgacaccacg gtgaccttgt cggcgacgat gaaggcggag tagaagccga caccgaactg
     1561 gccgatgagg tgggcgtcct tcttctggtc gccggtaagc ttgccgaaga actccttggt
     1621 acccgacctg gcgatggtgc ccaggtgggc gatcgcctcg tcgcggctca tgccgatgcc
     1681 gttgtcggcg atggtgatcg tcctggcctc ggcgtcgaag ccgatgcgga tcttcagctc
     1741 gctgtcgtcc tcatacagcg cgccgttgtc gagcgcctcg aagcgcagct tgtcgcaggc
     1801 atccgaggcg ttggagacga gctcgcgcag gaagatctcg cggttggaat acagcgagtg
     1861 gatcatcagg tgaagcagct gcttcacctc ggcctggaag gcgagcgtct gggaggaagg
     1921 ttgcgaaggg ttgtggactt cggacattgg aagctgctcc gtcgaggggt tgtcgaaacg
     1981 cagcagcagt tgggggcacg gcggcggatt tcaagcgcca ccgcccccgc ggatcagcag
     2041 cggaagcgcc cgaccagggc gtcgagctcg gcggccaggc cctgcagctg ctcggccgcg
     2101 accaccacgc tgtgcatcga gcccgaggtg ccgtcggcca tctcggtgat ggtctgcacc
     2161 tggcgggcga tctcggtgga ggcgctgctc tgctcgccgg tggcgtaggc cacctcgcgg
     2221 atgcgctcga gcgcctcgcc cgcggtgctg cggatgcggt gcagcgaatc ggtcgcgtcg
     2281 cccaccagcg ccacgccgcc atcgacctgg ctcgacacct cggccatcgc gcccacggtg
     2341 gcgcgggtct cgttctggat gccctcgatc accttctcga tctgcacggt ggccaccgcg
     2401 gtgcgctcgg ccaggccgcg cacctcgtcg gccactaccg cgaagccgcg cccctgctcg
     2461 ccggcgcgcg cggcctcgat ggccgcgttg agcgcgagca ggttggtctg gccggcgatc
     2521 tccttgatca cgttggcgat ggtgccgacc tcgtcggcgc gtgccacgag ctgctggatc
     2581 ttctgcgccg cgtcgcccac gcgcgcggcg atggcctgca tctcgcgcgc cgcgcgctcg
     2641 gccttgccgg cgccctcgtc ggcgagcccg gccgcggtgc tcgaatgctg ctcggccagg
     2701 cgcgcactgt cggagatctg ctcgatgctc acggtcatct cctcgatggc ggcggcgatc
     2761 gacgcggtcg cgccggtctg cgagaccgag gcctcggaca ccgagcgcga caccgcgtgg
     2821 atctcgcgcg cgctcgcgcc caggcgcgag gcgctgccgc cgatagcgcc gatcatggcg
     2881 cgcagctgcg cgagcatgtt cgagagattg ccgagcaggc tgtcgccccg cccgcgctgc
     2941 tccacgtcca ccgccagatc gccggcagcg gcgcgcttca tgatcgcgcc ggcgtaggcc
     3001 ggctcgccac cgagcgcgcc ggtgaccccg cggatcaccc acacgccgat cagcccgagc
     3061 agcacgagca caaccgcgac cagggcgccg aagcgcagcg ccgcgtcatt gaaggcagca
     3121 tcgatgtcat ccacatacac gcccgagccc accacccagc cccaggacgg gaaggccttc
     3181 acgtagctca gcttgggctg ggccgggtcg gtggcgacgc ccgagcgcgg ccaggtgtag
     3241 tccacgaagc cctgcccctg cgcgcgcgcc agcgtcacca tctcgcgcac gaagtgcttg
     3301 cccgcgctgt ccttcaggtc ggcgagcttg ccctcgagcg cgggccgcag cgggtgcatc
     3361 agcatgttca gctcgagatc gagcacgaag aagtactcgt tgttctggta gcgcagcccg
     3421 cgcagcgtgt ccttcgcagc cttctgcgcc gcctcgcggt cgagcacgcc ggcctgctcc
     3481 agcgcgtgga aatgcgcgag caccccggtg gccagttcga tctggctgcg cacgttgcgc
     3541 gcatagccct cgcgcatgtc gtcgcggttt tcgtacaagg ccgcgagcgc gagcaggacc
     3601 atgcccgcga gggcgacgag cagcatcagc gccaggcggc gggcgacggt gagggattcg
     3661 aaacgcatct tgccggacca tccacgaaat ggagactttg cgggagggct gtcgcctggg
     3721 cgtacaaccg cttcgtccgg tcaacggcag cattcggccc gacttgagca ccctgcagcc
     3781 tgaatgccac tccaccgttc agcggtattc caccgcgccg atctcgatct cgcgcacgcc
     3841 ggccgggctg tggaagcgca ccacgtcgcc gacgcgcgcc ttgagcaccg cacgcgccag
     3901 gggcgagatc cagctgatgc gcccctgcga ggcgtcggcc tcgtcgaccc cgacgatctg
     3961 cagggtctgc tcctccgcgg tgtcgagatc gctgaagctc accgtcgcac cgaagaagac
     4021 ctgctccagg ccctgctggc gctcgggatc gaccacctcg gcgctctcga tgcgcttgat
     4081 caggaagcgg atgcggcggt cgatctcgcg caggcgcttc ttgccgtaga tgtagtcgcc
     4141 gttctccgag cggtcgccgt tgcccgcggc ccaggccacg gtctccacca gcctggggcg
     4201 ctcgacgcgc acgagctggt cgagctcgac gcgcagcgca tcgtagccgc gccgggtcat
     4261 gtagttcttg ctgccggg
//
LOCUS       DAOQBS010000191         4265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_22262_length_4265_cov_16.147981, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000191 DAOQBS010000000
VERSION     DAOQBS010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22262_length_4265_cov_16.147981"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /gene="flgJ"
                     /locus_tag="PKN25_14470"
     CDS             <1..795
                     /gene="flgJ"
                     /locus_tag="PKN25_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HNV92153.1"
                     /translation="LSEMLYRQLGGKAAVRPDAAGEAGKTGFDLADVRRRPAIPAVLA
                     RDAESAAAPRLAALPAEARVPALGAAATAGEFSALSVYERAALQTRLDAAVRSARDAG
                     GAVSKSAQDFVTEVWPHAQEASRRTGIPAEFMVAQAALETGWGQKQLRHEDGSPSHNL
                     FNIKAGSAWSGRTVAREVTEYADGQAYTEAAHFRSYGSYAESFRDYAQLMTRSPRYAG
                     VLGQTDAAAFARGLQDAGYATDPMYADKLTRIIGGNTLRSALAMAG"
     gene            877..2859
                     /gene="flgK"
                     /locus_tag="PKN25_14475"
     CDS             877..2859
                     /gene="flgK"
                     /locus_tag="PKN25_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNV92154.1"
                     /translation="MASLLNIGLTGLSASQAYLNTTSHNIANAATPGFHRQTVTQEAR
                     DPQYSGGAFFGTGTGVTGVARSYSQLLEGQVLSADSRRAEYAAYSQFISQLDNVLADA
                     DTGLTPALNEFFGALQNVTSNPTNVAARQALLSSGDTLSARFRTLDGRISEIAEGVET
                     EMRSALESINGYAAAIAELNERMVISRTGGAGSAANDLLDQRDQAIAELNRLVKVSTV
                     EQRDGSTSVFIGSGQSLVMGNRAKQLELGDQLDENGRSMITVRANSGAVEPLAESLVG
                     GGTLGGLLAARREGTASAQRSLGLLATTLATEFNSQHALGLDLEGRLGGDFFKIGGVR
                     VDPSDSGLSVRIDPGQVSSLTGSDYSLRYDGAAFQLTDLATGNAVPAAQMQLVTEADG
                     STTIRAQGLVFTGARSTLTEPAFVYPTRYTAGAIEVANRDPRAVAAAAPVLASPTRGD
                     GSSLRIEQSGLLSRGSAAAGTELGLPALKYTFNAVDKRFELDAAAPGAAGWPATLAYD
                     PATSAAGESFTLEGPGGVRLELKISGTPAANDVIALADNKSGVADNRNAALLGALQTD
                     KLLFGSGGQATATLNNAYAQLVAKVGSKTREVQAGERTQTSLLDQAKASRDAVSGVNL
                     DEEAANLVRFQQSYQAAAKVMGVAQRLFDEILAIAR"
     gene            2874..3782
                     /gene="flgL"
                     /locus_tag="PKN25_14480"
     CDS             2874..3782
                     /gene="flgL"
                     /locus_tag="PKN25_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNV92155.1"
                     /translation="MRVSTNMIYDQGVIALQKQSAALLHTGQQIATGRKILTPSDDPV
                     ASARALDVSQSKSVNQQFITNQGYADDALKLVDGQLDGAGEIIQYARERVLAAGNPAL
                     SPSDRQSIASDLRSQFDAMLAIANTRDANGDYLFAGYRSNTPPFEKTVTAGTLGSDYK
                     GDQGSRTMQVSSSRFMPISFGGESVFGSEGDGVFKVLGEFISALEGGGDVSGAVSTAL
                     GGLDDSLDRTLTVRAQIGSQQVEVEQLGFIGSDLDLQYQTTLSNLQDLDYNEAISRLT
                     QQRTFLEAAQQSFLKTTGLSLFNYLS"
     gene            3788..4264
                     /locus_tag="PKN25_14485"
     CDS             3788..4264
                     /locus_tag="PKN25_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92156.1"
                     /translation="MRRALLVLAACSVLGCASEREGTGLLDRAAAAPGPIAGAVGAVW
                     MASELSYLEPEYLAGMLVAYGIYDPLAPTWSIEVSRSGEDRVRMDLHMKRMTTGGDGE
                     ARRVFLRNAREIVRAGGFAGFDELSWEEGIESTRPFARRVASGEIRLVRSRTFPEF"
BASE COUNT          684 a         1538 c         1443 g          600 t
ORIGIN      
        1 ctgtccgaga tgctctaccg ccagcttggc ggcaaggctg cggtgcggcc cgacgcggca
       61 ggcgaggccg gcaagaccgg cttcgacctt gccgacgtgc gtcgccgccc ggcaattcct
      121 gccgtgctcg cgcgcgatgc ggaaagtgct gccgccccgc gccttgccgc cttgccggcc
      181 gaggcgcgcg tgcccgcgct gggcgccgcc gcgaccgcag gggagttctc cgcgctgtcg
      241 gtgtacgagc gcgctgcgct gcagacccgc ctcgacgccg cggtgcgcag cgcgcgcgat
      301 gccggcgggg cggtatcgaa gtccgcgcag gacttcgtca ccgaggtgtg gccgcacgcg
      361 caggaagcca gccgccgcac cggcatcccg gccgaattca tggtcgccca ggcggcgctc
      421 gagaccggct ggggacagaa gcagttgcgt cacgaggacg gcagcccgag ccacaacctc
      481 ttcaacatca aggccggcag tgcgtggtcg gggcgtaccg tcgcgcgcga ggtcaccgag
      541 tacgccgacg gccaggccta caccgaggct gcgcacttcc gttcctacgg ctcgtatgcc
      601 gagtccttcc gcgactatgc ccagctgatg acgcgcagcc cgcgctacgc cggcgtgctc
      661 ggccagaccg acgcggcggc cttcgcgcgc ggcctgcagg acgccggcta cgccaccgac
      721 ccgatgtatg ccgacaagct cacccgcatc atcggcggca ataccttgcg cagtgcgctg
      781 gcaatggcgg gctgaggacc ggcggtcgag gtgcgtatcg agcgctggca aggatcttgc
      841 atgtagaggc tcgaaccagc gcatcaggga aacatcatgg cgtccttgct caatatcgga
      901 ctgaccgggc tcagcgcctc ccaggcctac ctcaacacca ccagccacaa catcgccaac
      961 gcggcgacgc cgggcttcca tcgccagacc gtcacccagg aagcgcgcga cccgcaatac
     1021 agcggcggcg ccttcttcgg caccggtacc ggcgtcaccg gcgtggcgcg cagttacagc
     1081 cagctgctcg agggccaggt gctgagcgcc gacagccggc gtgcggaata cgccgcctac
     1141 agccagttca tcagccagct cgacaacgtg ctcgccgacg ccgacaccgg cctcacgccc
     1201 gcgctcaacg aattcttcgg tgcgctgcag aacgtcacct ccaacccgac caacgtggcc
     1261 gcgcgccagg cgctgctctc cagcggcgat accctgtcgg cgcgcttccg caccctggac
     1321 gggcgtatct cggagatcgc ggaaggcgtc gagaccgaga tgcgcagcgc gctggagagc
     1381 atcaacggct acgccgccgc gatcgccgag ctcaacgagc gcatggtgat ctcgcgcacc
     1441 ggcggcgccg gttcggccgc caacgacctg ctcgaccagc gcgaccaggc gatcgccgag
     1501 ctcaaccgcc tggtcaaggt gagcacggtg gagcagcgcg acggttcgac gtcggtcttc
     1561 atcggttcgg ggcagagcct ggtcatgggc aatcgcgcca agcagctcga attgggcgac
     1621 cagctcgacg agaacgggcg ctcgatgatc acggtgcgcg ccaactcggg ggcggtggag
     1681 ccgctcgccg agagcctcgt cggtggtggc acgctcggcg gcctgctggc ggcgcgccgc
     1741 gagggcacgg cctcggcgca gcgcagcctc ggcttgctgg cgaccacgct ggcgaccgag
     1801 ttcaatagcc agcacgcgct cggcctcgac ctggaaggcc ggctgggggg agatttcttc
     1861 aagatcggcg gggtgcgcgt cgatccgtcg gacagcggcc tgagcgtccg gatcgacccc
     1921 gggcaggtct cctcgctcac cggcagcgac tactcgctgc gctacgacgg cgcggccttc
     1981 cagctcaccg atctcgccac tggcaacgcc gtgcccgccg cgcagatgca gctcgtgacc
     2041 gaggcggacg gttcgaccac catccgggcg caggggctcg tctttaccgg cgcgcggagc
     2101 accctgaccg agccggcctt cgtctatccc acccgttaca ccgcgggggc gatcgaggtg
     2161 gcgaaccggg acccgcgcgc cgtcgcggcc gcggcgccgg tgctggccag tccgaccagg
     2221 ggcgacggca gctcgctgcg gatcgagcaa tccgggctgc tcagccgcgg cagtgcggcg
     2281 gccggcaccg agctcggcct gcccgccctg aagtacacct tcaacgcggt ggacaagcgc
     2341 ttcgaactcg acgcggctgc gccgggggcg gccggctggc ctgcgaccct ggcctacgac
     2401 ccggcgacca gcgccgcggg cgagtccttc acgctggagg ggcccggagg cgtccggctc
     2461 gagctcaaga tctccggcac ccctgccgct aacgatgtca tcgcgctcgc cgacaacaag
     2521 agcggcgtcg ccgacaaccg caacgctgcg ctgctcggcg cgctgcagac cgacaagctc
     2581 ttgttcggca gcggcggcca ggccaccgcc acgctcaaca acgcctatgc gcagctggtg
     2641 gccaaggtcg gcagcaagac gcgcgaggtg caggccggcg aacgcaccca gacctcgctg
     2701 ctcgaccagg ccaaggcttc gcgcgacgcg gtctcgggcg tgaacctgga cgaggaggcg
     2761 gccaacctgg tgcgcttcca gcagagctac caggctgcgg ccaaggtgat gggcgtggcg
     2821 cagcgcctgt tcgacgaaat actggcgatc gcccgctgag gagagagcac accatgcgcg
     2881 tatcgaccaa catgatctac gaccagggcg tcatcgccct gcaaaagcag tccgccgcgc
     2941 tgttgcatac cggccagcag atcgccaccg gccgcaagat cctcacccct tcggacgatc
     3001 cggtggcctc cgcgcgcgcg ctcgatgtca gccagtcgaa gagcgtcaac cagcagttca
     3061 tcaccaacca gggctatgcc gacgatgcgc tcaagctggt cgacggccag ctcgacggag
     3121 ccggcgagat catccagtac gcgcgcgagc gcgtgctcgc ggctggcaat cccgcactgt
     3181 cgccgtccga ccgccagtcg atcgcctccg acctgcgcag ccagttcgat gccatgctgg
     3241 cgatcgccaa cacgcgcgac gccaacggcg actacctgtt cgccggctac cggtcgaaca
     3301 cgccgccgtt cgagaagacg gtcactgccg gcacgctcgg cagcgactac aagggcgatc
     3361 agggttcgcg caccatgcag gtttcgtcct cccgcttcat gccgatcagc tttggtggcg
     3421 agtcggtgtt cgggagcgag ggcgacgggg tgttcaaggt gctgggcgag ttcatctccg
     3481 ccctcgaagg cggaggcgat gtgagcggcg cggtgagcac ggcgctgggc ggtctcgacg
     3541 acagcctgga ccgcacgctc accgtgcgcg cgcagatcgg ctcgcagcag gtcgaggtcg
     3601 agcagctcgg cttcatcggc agcgatctcg acctgcagta ccagaccacg ctgtccaacc
     3661 tgcaggacct cgactacaac gaggcgatct cgcgcctgac ccagcagcgc accttcctcg
     3721 aagccgccca gcagtccttc ctcaagacga ccgggctgtc gctgttcaac tacctcagct
     3781 gaccgccatg cgccgcgcgc tcctcgtcct tgccgcctgt tccgtcctcg gctgcgccag
     3841 cgagcgcgag ggcaccggcc tgctcgaccg cgccgccgcg gccccagggc cgatcgccgg
     3901 tgcggtgggc gcggtgtgga tggcgagcga gctttcctac ctcgagccgg agtacctcgc
     3961 cggcatgctg gtggcctacg gcatctacga tccgctcgcg ccgacgtggt cgatcgaggt
     4021 cagccgcagc ggcgaagacc gtgtgcgcat ggacctgcac atgaagcgga tgaccaccgg
     4081 cggcgacggc gaggcacgcc gggtcttcct gcgcaacgcg cgcgagatcg tgcgtgcggg
     4141 cggtttcgcc ggcttcgacg agctgagctg ggaggagggc atcgagtcca cccggccttt
     4201 cgcccgccgc gtcgccagcg gcgagatccg cctggtgcgt tcgcgcacct tcccggagtt
     4261 ctgag
//
LOCUS       DAOQBS010000192         4184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_22887_length_4184_cov_16.297409, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000192 DAOQBS010000000
VERSION     DAOQBS010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22887_length_4184_cov_16.297409"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PKN25_14490"
     CDS             <1..944
                     /locus_tag="PKN25_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNV92157.1"
                     /translation="TLAALYDEPYADSSAIPTYRVCQLARKQVTVALSGDGGDESFGG
                     YRRYRMHLMEEKMRAALPMGVRRPLFGLLGRVYPKADWAPRVFRAKTTFQAMARDATE
                     AYFHSVSILRGDMRRKLFSAEFRNRLGGYDALEVFRRHAAAAGTDDALALIQYLDLKT
                     YLVGDINTKVDRASMAHSLEVREPLMDHKLVEWLATLPSGLKIQGNEGKYLFKKAMEP
                     LLPDDVLYRPKMGFAVPLERWFRGPLKQRVRDAVLGETLAATGIFERSYLEHLVDAHQ
                     SGARDYSAPLWTLLMFEAFVRNTAAGATLPVALAEAV"
     gene            966..2177
                     /locus_tag="PKN25_14495"
     CDS             966..2177
                     /locus_tag="PKN25_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase, exosortase A
                     system-associated"
                     /protein_id="HNV92158.1"
                     /translation="MRILHILDHSIPLHSGYTFRTAAILREQRALGWETFHLTSPKQG
                     ETKAMVEEIEGLRFHRTGVPTPASSGIAELRQIRAVQARIEQLAAELRPDILHAHSPV
                     LNAIPAIRAGRKLGIPVVYEIRAFWEDAAVDHGTTTEGSLRYRATKALETWAIKRADH
                     VFTICEGLRADIVGRGIPAAKVTVIPNAVDIESFQLSGDADPALREQLGLAGTTVVGF
                     VGSFYAYEGLDLLLEAFPALLQKRPELRLLLVGGGPQDENLKAQALRLGVADKVVFTG
                     RVPHKDVSRYYDQIDLLAYPRHSMRLTELVTPLKPLEAMAQGRLFVASDVGGHKELIR
                     DGETGRLFKAGSAEALAAAIDDLLAHRERWPAMRAAGRQFVEDVRNWTNSVANYTPVY
                     RSLVAKPRAAA"
     gene            2174..3271
                     /locus_tag="PKN25_14500"
     CDS             2174..3271
                     /locus_tag="PKN25_14500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV92159.1"
                     /translation="MNFSALRVLLVGPLPPPAGGMANQTRQLAELLRGEGASVEVVQV
                     NAPYRPAWVERLKGVRALFRLLPYLLRLWQAAGRANLMHVMANSGWSWHLFAAPAVWI
                     GWLRGVPVVVNYRGGEAAGFLARSAAVVRATLRRASSLVLPSGFLLEVFARHGMAGRI
                     VSNIVDLERFHPATAPRAAGEGPHLVVARNLEALYGNDTALRAFARLCAAWPAARLSI
                     AGSGPEAAALAALAQELGIAERVRFTGRLDREQMAALYRDADLMLNPSRVDNMPNAIL
                     EALASGLPVVTTDVGGIPFIVTQGHTAMLVPPDDPQAMADAAHKVLADTGLRAALVAA
                     GCAEVQRYRWSSVRAQLLSAYADAIAAKRAT"
     gene            3268..>4184
                     /gene="xrtA"
                     /locus_tag="PKN25_14505"
     CDS             3268..>4184
                     /gene="xrtA"
                     /locus_tag="PKN25_14505"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HNV92160.1"
                     /translation="MNMKGTAMLQSVRPGAMPTHLQATDARPATLGDPRTRRLHLAVL
                     ALGLLWLLFWYRDTFLAMVGIWDRSDTFAHGFVIAPISAWLVWRRRHFIDHVPIAPSL
                     LGICAGLVAGAAWLLGELASVDAVSQFAFVGMLVGFVWAVMGSAVVRTYAFPIGFLFF
                     MVPFGEFLFPTMMQWTADFIIWAVRASGVPVYAEGFLLVIPSGRWQVVEGCSGVRYLM
                     ASIVVGSLYAYLNYRSTQKRLLFVAASIVLPVFANWLRAYGIVMLGHLTDNRLAAGAD
                     HLIYGWVFFGIIILALFWIGSRWQEDEEEA"
BASE COUNT          583 a         1546 c         1383 g          672 t
ORIGIN      
        1 acaccctggc cgcgctctac gacgagccct acgccgacag ctcggccatc cccacctacc
       61 gcgtgtgcca gctcgcgcgc aagcaggtca ccgtggcgct gtcgggcgac ggcggcgacg
      121 agagcttcgg cggctaccgc cgctaccgca tgcacctgat ggaagagaag atgcgcgccg
      181 cgctgccgat gggcgtgcgc cgaccgctct tcggcctgct cggccgcgtc tatcccaagg
      241 ccgactgggc gccgcgcgtg ttccgcgcca agaccacctt ccaggcgatg gcgcgcgacg
      301 ccaccgaggc ctacttccac agcgtctcca tcctgcgcgg cgacatgcgg cgcaagctgt
      361 tcagcgccga attccgcaac aggctcggcg gttacgacgc gctcgaggtc ttccgccgcc
      421 atgccgcagc cgccggcacc gacgatgcgc tcgcgctgat ccagtacctc gacctcaaga
      481 cctacctggt cggcgacatc aacaccaagg tcgaccgcgc gagcatggcg cactcgctcg
      541 aggtgcgcga gccgctgatg gaccacaagc tggtcgagtg gctggcgacg ctgccctccg
      601 gcctcaagat ccagggcaac gagggcaagt acctgttcaa gaaggcgatg gagccgctgc
      661 tgcccgacga cgtgctctac cgcccgaaga tgggcttcgc ggtgccgctc gagcgctggt
      721 tccgcggccc gctaaagcag cgcgtgcgtg acgcagtgct gggcgagacc ctggccgcta
      781 cgggtatctt cgagcgcagc tacctcgagc atctggtcga cgcccaccag tccggcgcgc
      841 gcgactacag cgccccgctg tggacgctgc tgatgttcga ggctttcgtc cgcaacaccg
      901 cagccggcgc caccctgccg gtcgcgctcg ccgaggcggt ctgaacgcga cgttccgaat
      961 actccatgcg catcctgcac atcctcgatc actcgatccc gctgcacagc ggctacacct
     1021 tccgcaccgc ggcgatcctg cgcgagcagc gcgcgctcgg ctgggagacc ttccacctga
     1081 cctcgcccaa gcagggcgag acgaaggcca tggtggagga gatcgagggc ttgcgtttcc
     1141 accgtaccgg cgtgccgacg ccggcgagca gcggcatcgc cgagctgcgc cagatccgtg
     1201 cggtgcaggc gcgcatcgag cagctcgcgg ccgaactgcg ccccgacatc ctccacgccc
     1261 actcgccggt gctcaacgcc attccggcca tccgcgccgg gcgcaagctc ggcatcccgg
     1321 tcgtctacga gatccgcgcc ttctgggaag acgccgcggt cgaccacggc accaccaccg
     1381 agggcagcct gcgctaccgc gccaccaagg cgctcgagac ctgggcgatc aagcgcgccg
     1441 accacgtctt caccatctgc gaaggcctgc gcgccgacat cgtcggccgc ggcatcccgg
     1501 cggccaaggt cacggtcatc cccaacgcgg tcgacatcga gtccttccag ctctcgggcg
     1561 acgccgaccc tgccttgcgg gagcagctcg gcctcgccgg caccaccgtg gtgggcttcg
     1621 tcggctcctt ctacgcctac gagggcctcg atctgctgct cgaggccttc cccgcgctgc
     1681 tgcagaagcg ccccgagctg cgcctgctgc tcgtcggcgg cgggccgcag gacgaaaacc
     1741 tcaaggctca ggccctgcgc ctcggcgtgg ccgacaaggt cgtcttcacc ggccgcgtgc
     1801 cgcacaagga cgtcagccgc tactacgacc agatcgacct gctcgcctat ccgcgccact
     1861 ccatgcgact caccgagctg gtcaccccgc tcaagccgct cgaggccatg gcgcagggtc
     1921 ggctcttcgt cgcctccgac gtcggcggcc acaaggagct gatccgcgac ggcgagaccg
     1981 gcaggctgtt caaggccggc agcgccgagg cgctcgccgc cgccatcgac gacctgctcg
     2041 cgcaccgcga gcgctggccg gcgatgcgcg cggcggggcg gcagttcgtc gaggacgtgc
     2101 gcaactggac gaacagcgtg gcgaattaca cgccggtgta tcgcagcctg gtcgcgaagc
     2161 ctcgcgccgc ggcatgaatt tctccgcgct gcgcgtcctg ctcgtcgggc cgctgccgcc
     2221 gcctgccggc ggcatggcca accagacccg ccagctcgcc gagctgctgc gcggcgaggg
     2281 cgcgtcggtc gaggtcgtgc aggtcaatgc gccgtatcgt ccggcttggg tcgaaaggct
     2341 gaagggcgtg cgcgcgctgt tccggctcct gccctacctg ctgcggctgt ggcaggccgc
     2401 agggcgggcg aacctcatgc acgtgatggc caactccggc tggtcctggc atctcttcgc
     2461 cgcgccggcg gtgtggatcg gctggctgcg cggcgtgccg gtggtggtca attaccgcgg
     2521 aggcgaggcg gccggcttcc ttgcccgctc ggcggcggtc gtgcgtgcca ccctgcgccg
     2581 cgcgtccagc ctggtgctgc cgtccggctt cctgctcgaa gtcttcgcgc gccacggcat
     2641 ggccgggcgc atcgtctcca acatcgtcga cctcgagcgt ttccatcccg cgacggcgcc
     2701 gcgcgcggcc ggcgagggcc cgcatctggt cgtcgcccgc aacctcgaag ccctctacgg
     2761 caacgacacc gcgctgcgcg ccttcgcgcg gctgtgcgcg gcctggccgg cggcgcgcct
     2821 ctcgatcgcc ggcagcgggc cggaggccgc ggcgctggcc gcgctcgcgc aggaactcgg
     2881 catcgccgag cgggtccgct tcaccggtcg cctggaccgc gagcagatgg ctgcgctcta
     2941 ccgtgacgca gatctcatgc tcaacccgag tcgggtggat aacatgccca atgccatcct
     3001 cgaggcgctg gcgagcggcc tgccggtggt caccaccgac gtcggcggca ttcccttcat
     3061 cgtcacgcaa ggccacacgg ccatgctggt tccacccgac gatccgcagg cgatggccga
     3121 cgccgcgcac aaggtgcttg ccgacaccgg cctgcgcgcc gcgctggtcg ccgccgggtg
     3181 cgccgaggtg cagcgctacc gctggagctc ggtgcgcgca caactgctgt ccgcctacgc
     3241 ggatgcgatt gccgcgaaga gggccacatg aacatgaaag gaaccgcgat gcttcaatcc
     3301 gtgcggccgg gcgcgatgcc gacccacctg caggcgaccg acgcccgccc tgcgacgctc
     3361 ggtgatccgc gcacgcgcag gcttcacctg gcggtgcttg cgctgggact gctgtggctg
     3421 ctgttctggt atcgcgacac cttcctggcc atggtgggca tctgggaccg ctcggacacc
     3481 ttcgcccacg gcttcgtgat cgcgccgatc tcggcctggc tggtatggcg gcgccggcac
     3541 ttcatcgacc atgtaccgat cgcgccctcg ctgctcggca tctgcgccgg actcgtcgcc
     3601 ggcgcggcct ggttgctcgg cgagctcgcc agcgtcgatg cggtgtcgca gttcgccttc
     3661 gtgggcatgc tggtgggctt cgtctgggcg gtgatgggca gcgcggtggt gcgcacctac
     3721 gccttcccga tcggcttcct gttcttcatg gtgccgttcg gcgagttcct cttccccacc
     3781 atgatgcagt ggaccgccga cttcatcatc tgggcggtgc gcgcctccgg agtgccggtg
     3841 tacgccgaag gcttcctgct ggtgatcccc tccgggcgct ggcaggtggt ggaggggtgc
     3901 agcggcgtgc gctacctgat ggcctcgatc gtcgtcggca gcctgtacgc ttacctgaac
     3961 taccgcagca cgcagaagcg cctgcttttc gtcgccgcct cgatcgtcct gccggtattc
     4021 gccaactggc tgcgcgccta cggcatcgtg atgctcggcc acctcaccga caaccgcctc
     4081 gctgccgggg ccgaccacct gatctacggc tgggtcttct tcggcatcat catcctcgcg
     4141 ctgttctgga tcggttcgcg ctggcaggag gacgaggaag aggc
//
LOCUS       DAOQBS010000193         4164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_23056_length_4164_cov_15.078365, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000193 DAOQBS010000000
VERSION     DAOQBS010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23056_length_4164_cov_15.078365"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /gene="ppsA"
                     /locus_tag="PKN25_14510"
     CDS             complement(<1..1214)
                     /gene="ppsA"
                     /locus_tag="PKN25_14510"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNV92161.1"
                     /translation="MTRYVIPFNELRMSDVEKVGGKNASLGEMISQLPSSVRVPGGFA
                     TTAEAYREFLAHDGLADRINAALDALDVDDVDALAKTGAQIRQWIVDLPFPAKLEAEI
                     KTAYEAITAEGEGSFAVRSSATAEDLPDASFAGQQETFLNIHGYENILHAMKEVFASL
                     YNDRAIAYRVHKNFAHADVALSAGVQRMVRSDSGASGVMFSIDTESGFDQVVFITASY
                     GLGETVVQGAVNPDEFYVHKPTLALDKPAIIRRNLGSKLIKMVFTDKAVAGKSVRTVD
                     VPEADRNRFSLTDEDVLELARYAVIIEKHYGRPMDIEWGKDGVDGKLYILQARPETVK
                     SQDSGLVMEKYRLKQYGKALTHGRAIGQKIGAGTVRIVGDASEMNRVQAGDILVTDMT
                     DPNWEPVMKRASA"
     gene            1352..2185
                     /locus_tag="PKN25_14515"
     CDS             1352..2185
                     /locus_tag="PKN25_14515"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNV92162.1"
                     /translation="MNDIARRTVFFVSDGTGITAETLGHSLLAQFPEARFRQVRAPFI
                     DDIDKAIDCATQIREAAAEDGVRPIVFSTLVNQATVDALHKSDALFLDLFERFIGPLE
                     AELGQRSTHAVGRFHGIADSLNYKYRIEAINFAMAHDDGISSEGELAEADVILVGVSR
                     SGKTPTSLYLAMQFGVKAANYPLIPEDFERNKLPGELHKYRNKLFGLTIAPERLSQIR
                     QERRPNSRYAALENCRYEIDAAHKLMRRENIRCLDSTTKSIEEISATILQTIRVDRPG
                     F"
     gene            2292..3461
                     /locus_tag="PKN25_14520"
     CDS             2292..3461
                     /locus_tag="PKN25_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HNV92163.1"
                     /translation="MKRTRLKRRGGLGRTAEQLVWLAGGLAQSGSRIEDHYWETQLAR
                     LIDETLTDNDEEVLNAALDHLYSADARAYDELADNIESRAECAAGSLAEHDVVLIAAP
                     VLAWSRYRIPAANIPPAVLANLRVHLQAHVLAKGAKLSVADFLFSPDQLPQGYCATAE
                     FAKVICGAAADNLDLHIDTDGMPETAQFLSDTRYLLAAVAVPRGAALFRWQEPDGTRD
                     GALEQWRAQGGACLTPLLPGCAVDLVLPDSYFAASRAADKDSRPYSVRASVAFLATTL
                     ETPAANLRAVVAPFWERTLEEYRIGFTVRGRDEVVHGVVWPLLGAEDETTDCVAQIET
                     VLRESGLTDVVALEHHFPLEYCDDCGAPLYPSPEGEVVHAEMPEEQGVQAPRHLH"
     gene            3479..3880
                     /gene="msrB"
                     /locus_tag="PKN25_14525"
     CDS             3479..3880
                     /gene="msrB"
                     /locus_tag="PKN25_14525"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNV92164.1"
                     /translation="MAREVDKPDAEWRRQLSDLQYRVTREKGTERAFTGEYWDVWDKG
                     AYNCVCCGAPLFRSEHKFDAGCGWPSFWTAAEPDNVETAVDRSHFMVRTEVLCHQCGA
                     HLGHVFEDGPQPTGLRYCINSASVRLEKESD"
BASE COUNT          703 a         1503 c         1313 g          645 t
ORIGIN      
        1 gcgctggcgc gcttcatcac cggctcccag ttggggtcgg tcatgtcggt gacgaggatg
       61 tcgccggcct gcacgcggtt catctcggag gcgtcgccga cgatgcggac cgtgccggca
      121 ccgatcttct ggccgatcgc gcggccgtgg gtgagggcct tgccgtactg cttgaggcgg
      181 tacttctcca tcaccaggcc ggaatcctgg ctcttcaccg tctcgggacg cgcctgcagg
      241 atgtagagct tgccatcgac gccgtccttg ccccactcga tgtccatcgg gcggccgtag
      301 tgcttctcga tgatcaccgc gtagcgggcg agctcgagca cgtcctcgtc ggtcagcgag
      361 aagcggttgc gatcggcctc gggcacatcg accgtgcgca ccgacttgcc ggcgacggcc
      421 ttgtcggtaa acaccatctt gatgagcttg gagccgaggt tgcggcggat gatcgccggc
      481 ttgtcgagcg ccaacgtggg cttgtggacg tagaactcgt ccgggttcac cgcgccctgc
      541 acgacggtct cgccgaggcc gtaggaggcg gtgatgaaca ccacctggtc gaagcccgat
      601 tcggtgtcga tcgagaacat cacgcccgag gcgcccgaat ccgagcgcac catgcgctgc
      661 acgccggccg acagcgcgac gtcggcgtgg gcgaagttct tgtgcacgcg gtaggcgatc
      721 gcgcggtcgt tgtacagcga cgcgaacact tccttcatcg catgcaggat gttctcgtag
      781 ccgtggatgt tgaggaaggt ctcctgctgg ccggcgaagg aggcgtccgg caggtcctcg
      841 gcggtcgccg acgagcgcac ggcgaagctg ccctcgcctt cggcggtgat ggcctcgtag
      901 gcggtcttga tctcggcctc gagcttggcc gggaagggca ggtccacgat ccactggcgg
      961 atctgcgcgc cagtcttggc cagggcatcg acgtcatcca cgtcgagcgc gtcaagcgcg
     1021 gcgttgatgc ggtcggccag gccgtcgtgg gcgaggaact cgcgataggc ctcggcggtg
     1081 gtggcgaagc cgcccgggac acggacgctg gaaggcagct ggctgatcat ctcgccgagc
     1141 gaggcgttct tgccgccgac cttctcgacg tcggacatgc gcagttcgtt gaaagggatc
     1201 acgtagcgtg tcatggatgg cttccgcaaa tgttggggaa aaagcaaaat atcgcgaacg
     1261 gcagtgtacg attttttgtg ctgtgccgca tcacagggtt tgccccaata cacgccccgc
     1321 acccctggtc ggggccgcaa ggtccgaaat catgaacgac atcgcccgcc gcaccgtctt
     1381 cttcgtctcc gacggcaccg gcattaccgc cgaaaccctt ggccacagcc tgttggcgca
     1441 gtttcccgag gcgcgcttcc gccaggtgcg cgcccctttc atcgacgaca tcgacaaggc
     1501 gatcgactgc gcgacgcaga tccgcgaggc cgcggccgag gacggcgtgc gcccgatcgt
     1561 gttcagcacg ctggtcaatc aggccacggt tgatgcactg cacaaatccg acgcgctctt
     1621 cctggacctc ttcgagcgct tcatcggccc gctcgaagcc gagctcggcc agcgctccac
     1681 ccatgccgtg ggccgcttcc atggcatcgc cgacagcctc aactacaagt accgcatcga
     1741 ggcgatcaac ttcgccatgg cacacgacga cggcatctcg agcgagggcg agctcgccga
     1801 ggccgacgtg atcctggtcg gcgtgtcgcg ctcgggcaag acccccacca gcctctacct
     1861 ggcgatgcag ttcggcgtca aggcggccaa ctacccgctc atccccgagg atttcgagcg
     1921 caacaagctc cccggcgaac tgcacaagta ccgcaacaag ctcttcggcc tcaccatcgc
     1981 ccccgaacgc ctgtcgcaga tccgccagga acgccgcccc aacagccgct acgccgcgct
     2041 ggagaactgc cgctacgaga tcgacgccgc gcacaagctc atgcgccgcg agaacatccg
     2101 ctgcctcgac tccaccacca agtcgatcga ggagatctcc gccaccatcc tgcagaccat
     2161 ccgggtcgac cgccccggtt tctgagcgcg cgccgggcgc ttcgaccggc gttttcccgc
     2221 ggcattggta gaatacgcgg tcatgcgtgc cgcccaagcc ggcaccccga acagcgcaac
     2281 aggaacccga catgaaaaga acgcgcctca agcgccgcgg cggtctcggc cgcactgccg
     2341 aacaactcgt ctggctcgcc ggcgggctgg cccagtccgg cagccggatc gaggaccact
     2401 actgggagac gcagctcgcc aggctgatcg acgagaccct gaccgacaac gacgaggaag
     2461 tgctcaacgc cgcgctcgac cacctctaca gcgccgacgc gcgcgcctac gacgagctcg
     2521 ccgacaacat cgagtcgcgc gccgaatgcg ccgccggcag cctcgccgag cacgacgtcg
     2581 tgctgatcgc cgcgccggtg ctggcctggt cgcgctaccg catccccgcc gccaacatcc
     2641 cgcccgcagt gctcgccaac ctgcgcgtgc acctgcaggc ccacgtgctc gccaaggggg
     2701 ccaagctgtc ggtggccgac ttcctcttca gccccgacca gctgccccag ggctactgcg
     2761 ccaccgcgga gttcgccaag gtgatctgcg gtgcggcggc cgacaacctc gacctgcaca
     2821 tcgacaccga tggcatgccg gagaccgcgc agttcctctc cgacacccgc tacctgctcg
     2881 ccgcggtggc cgtgccgcgc ggcgccgcgc tgttccgctg gcaggagccg gacggcaccc
     2941 gcgacggcgc gctcgagcag tggcgcgccc agggtggcgc ctgcctgacc ccgctgctgc
     3001 ccggctgcgc ggtcgacctc gtgctgcccg actcctactt cgccgccagc cgcgccgccg
     3061 acaaggacag ccggccctac tcggtgcgcg cctcggtcgc cttcctcgcc accacgctgg
     3121 aaacacctgc cgccaacctg cgcgcggtgg tcgcgccgtt ctgggagcgc actctcgagg
     3181 aataccgcat cggcttcacc gtgcgcggcc gcgacgaggt cgtgcacggg gtggtgtggc
     3241 cgctgttggg ggccgaggac gagaccaccg actgcgtcgc gcagatcgaa accgtattgc
     3301 gcgaatccgg tctcaccgac gtcgtggcgc tcgagcacca cttcccgctc gagtactgcg
     3361 acgattgcgg cgccccgctc tacccctcgc ccgagggcga ggtcgtgcat gccgagatgc
     3421 ccgaggaaca gggcgtgcag gcgccgcgcc acctccactg atcccgcacg aggttcccat
     3481 ggcccgcgaa gtcgacaaac ccgacgccga atggcgccgc cagctctccg acctgcagta
     3541 ccgcgtcacc cgcgagaaag gcaccgaacg tgccttcacc ggcgaatact gggacgtctg
     3601 ggacaagggc gcctacaact gcgtgtgctg cggtgccccg ctcttccgct ccgagcacaa
     3661 gttcgacgcc ggctgcggct ggccgagctt ctggaccgca gccgagcccg acaacgtcga
     3721 gaccgcggta gaccgcagcc acttcatggt ccgcaccgag gtcctttgcc accagtgcgg
     3781 cgcacacctc ggccacgtct tcgaggacgg cccgcagccc accgggctgc gctactgcat
     3841 caactcggcg tcggtgcgcc tggagaagga gtcggactga agatcgcgac tcgcgtcgct
     3901 cccacaaaac ccgcacagct ccgcagcccc ccggaggagc aacacaagtc gcgatcgagg
     3961 ccccccgtag gagcgacgca agtcgcgatc gagaccccca gaccgccccg taggagcgac
     4021 gcaagtcgcg attgccagtt cagcgcgccc gaaacgaaaa aacccgcgcc agcctctcgg
     4081 ccgcgcgggt ttttcttgcg acgaggacga cgcttacacg cgttcccaca ccgtggtgat
     4141 gccctggccg ccgccgatgc acat
//
LOCUS       DAOQBS010000194         4156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_23127_length_4156_cov_14.101926, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000194 DAOQBS010000000
VERSION     DAOQBS010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23127_length_4156_cov_14.101926"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PKN25_14530"
     CDS             complement(<1..707)
                     /locus_tag="PKN25_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV92165.1"
                     /translation="MTKELAELLARAEGVLARLETLLPGPAAAPDWDAAVAFRWQRRH
                     GRTALRPVRAPHRIMLADIQDVDEQKARIDRNTRQFLAGRRANNVLLTGARGTGKSSL
                     IKALLNEYAARGLRLIEVDKTDLVDLPEIVELVAGRPERFILFCDDLSFEEGEDAYKA
                     LKSVLDGSVSAMSDNVLIYATSNRRHLMPEYHDENLQARHVDGEVHPGEAVEEKISLS
                     ERFGLWISFYPFSQDEYL"
     gene            complement(688..822)
                     /locus_tag="PKN25_14535"
     CDS             complement(688..822)
                     /locus_tag="PKN25_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92166.1"
                     /translation="MWLIFLELGVALALLLVVGLALRQSSAHARDRDDCGNPDDQGTR
                     "
     gene            1124..2143
                     /locus_tag="PKN25_14540"
     CDS             1124..2143
                     /locus_tag="PKN25_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HNV92167.1"
                     /translation="MEGWQRDELSAAWPALRASCSTLVRQAPWKAACEEAARLGGAPD
                     GGTVRRFLESRFTPWQVVNPDGSANGLVTGYYEPLIRGSRTRGGRSTWPIHGVPQDML
                     TIELADVYPELKSLRLRGRLVGNKVLPYWNRQQLSEREVPAPVLLWADDPIELFFLQV
                     QGSGRVQLPDGSLVRVGYADQNGHPYQSIGRWLVAQGELTLDKASMDGIKRWAQDNPR
                     RLQELLNANPSYVFFRELPASGGGPVGALGVPLSEGRSIAVDPRYVPLGAPVFLSTTQ
                     PLSERPLERLVMAQDTGGAIKGAVRADFFWGFGAEAGREAGKMRQQGRMWVLLPRGMK
                     PPMPH"
     gene            complement(2192..2920)
                     /locus_tag="PKN25_14545"
     CDS             complement(2192..2920)
                     /locus_tag="PKN25_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNV92168.1"
                     /translation="MKALSVPSLIRPLGLALALGATTFAHADEAGVKKSMEAFIGSPA
                     VERVTRTDYAGLYEVVLKNGQLVYTDAKNSFIIDGSIIDTATRRDVTQARLNQLSAID
                     FSTLPLDHAVKVVKGKGTRVIATFEDPNCGYCKRLGKELAQMDDVTVYTFLYPILSED
                     SRVKSDNIWCAKDKGRAWTDWVVDGKEPAKASCDTATITRNVELGQSLRISGTPTIFL
                     ADGSRIGGYLPRAELEKAMDAAKR"
     gene            complement(2938..4101)
                     /locus_tag="PKN25_14550"
     CDS             complement(2938..4101)
                     /locus_tag="PKN25_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HNV92169.1"
                     /translation="MKFDLIIVGGGLAGAALAVALRRSALRIAVIEHAVPRRPEGWDA
                     RVYAYSPVSARFLDQLGVWAHLDHQRLAEVAEMRIHGDAGGELVFSAYDSGLEELAWI
                     GESSLVHVEIWESLKRQHNVTLFCPAAPEALEIDEDVATLRLGDGRSLKAALVVGADG
                     RDSWVRERAGIAATITPYGERGVVANFVCERPSRGVAYQWFRADGVLAWLPLPGRHMS
                     MVWSAPDAFADELLGLEAEEFCARVEGAGGRALGKLELAGQRAAFPLRLMRVEETVRP
                     RVALIGDAAHAIHPLSGHGINLGFQDAKALAEVLDALPSWQDAGDLSVLRRYARMRAE
                     EPFLLQYTTHALSRLFGRQDPLSATLRNLGMNLTGRVPVLKNALVRYAANGRF"
BASE COUNT          643 a         1421 c         1443 g          649 t
ORIGIN      
        1 aggtactcgt cctggctgaa ggggtagaag gagatccaca ggccgaagcg ctcggagagc
       61 gagatcttct cttcgaccgc ctcgcccggg tgcacctcgc catcgacatg gcgggcctgc
      121 agattctcgt cgtggtactc cggcatcagg tggcggcggt tggaggtggc gtagatcagc
      181 acgttgtccg acatcgccga caccgagccg tcgagcacgc tcttcaaggc cttgtaggcg
      241 tcctcgccct cctcgaagga aaggtcgtcg cagaagagga tgaagcgctc cggccggccg
      301 gcgaccagtt cgacgatctc gggcaggtcg accaggtcgg tcttgtccac ctcgatcagg
      361 cgcaagccgc gcgcggcgta ttcgttgagc agggccttga tcagcgagga cttgccggtg
      421 ccgcgcgcgc ccgtgaggag cacgttgttg gcgcgccggc cggcgaggaa ctggcgggtg
      481 ttgcggtcga tgcgcgcctt ctgctcgtcc acgtcctgga tgtcggcgag catgatgcgg
      541 tgcggcgcac gcaccgggcg cagcgcggta cggccgtggc ggcgctgcca gcgaaaggcg
      601 accgcggcgt cccagtcggg cgcggcggca gggccgggca gcagggtttc gaggcgcgcc
      661 agcacgcctt cggcgcgggc gagcagctca gcgagttcct tggtcatcgg ggtttccgca
      721 atcgtcccgg tcgcgcgcgt gcgcggacga ctggcgcagg gcgagcccta cgaccagcag
      781 cagcgcgagc gcgacaccga gttcgagaaa gatcagccac accttccgtc tccggcagcc
      841 tgtgggggcc cggtatactg gcggcaaaga cagccgagtt caaccaatgc cccgcaagat
      901 gctctgccgc acccgcaacg ccctcttccc ttccctctcc gccctgctgg tggccgcttg
      961 cgccgccctg cccggcacgc ccgaaccctc cggagagcgc ccctgcgtac cctgtccgag
     1021 ctgcgcgccc tgctccggcg ccgcaccagc cgccacgccg ggcgctccgg ctgccccggc
     1081 cgccccagcc gcagccgcgc tgcaggcgag cgactggggc gcgatcgaag gctggcagcg
     1141 cgacgagctg tcggcggcct ggccggcgtt gcgcgcctcc tgcagcaccc tggtgcgcca
     1201 ggcaccgtgg aaggcagctt gcgaggaggc cgcccgcctg ggcggagcac ccgacggcgg
     1261 cactgtgcgt cgcttcctcg agagccgctt cacgccctgg caggtggtga atcccgacgg
     1321 cagcgcgaac gggctggtga ccggctacta cgagccgctg atccgtggca gtcgcacgcg
     1381 cggcggacgc agcacctggc cgatccatgg cgtgccgcag gacatgctga cgatcgagct
     1441 cgccgacgtg tatccggaac tgaagagcct gcgcctgcgc ggccgcctcg tcggcaacaa
     1501 ggtgctgccg tactggaacc gccagcagct ctccgaacgc gaggtcccgg caccggtgct
     1561 gctgtgggcg gacgatccga tcgaactctt cttcctgcag gtgcagggct cggggcgggt
     1621 gcagctgccc gacggcagcc tggtgcgggt cggctatgcc gaccagaacg gccatcccta
     1681 ccagtcgatc gggcgctggc tggtcgccca gggcgagctg acgctggaca aggcctcgat
     1741 ggatgggatc aagcgctggg cgcaggacaa cccgcggcgc ctgcaggaac tgctcaatgc
     1801 caacccgagc tacgtcttct tccgcgagtt gcccgcctcg ggcggcgggc cggtgggcgc
     1861 gctcggcgtg ccgctctccg agggccgcag catcgcggtg gatccgcgct acgtgccgct
     1921 gggcgcaccg gtgttcctct cgaccacgca gccgctcagc gagcgcccgc tcgagcgcct
     1981 ggtcatggcg caggacaccg ggggcgcgat caagggcgca gtgcgtgccg atttcttttg
     2041 gggattcggt gccgaggctg gccgcgaggc cgggaagatg cgtcagcagg ggcggatgtg
     2101 ggtgctgctg ccgaggggga tgaagccgcc catgccgcac tgacccgatc cgcggcgcgg
     2161 gcgtgaccgt ccgcccgcgc cctgcgaggg atcagcgctt ggcggcgtcc atcgcctttt
     2221 cgagttcggc gcgcggcagg tagccgccga tgcgcgagcc gtcggcgagg aagatcgtcg
     2281 gcgtgccgct gatgcggagg ctctggccga gttcgacgtt acgcgtgatg gtcgcggtat
     2341 cgcagctcgc cttggcgggc tccttgccat cgaccaccca gtcggtccac gccctcccct
     2401 tgtccttcgc gcaccagatg ttgtccgact tgacacgcga gtcctcgctc aggatcggat
     2461 acaggaaggt gtacacggtg acgtcgtcca tctgcgcgag ctccttgccc aggcgcttgc
     2521 agtagccgca gttgggatcc tcgaaggtgg cgatcacgcg cgtgcccttg cctttcacga
     2581 ccttcaccgc gtggtcgagc ggcagggtgg agaagtcgat cgcggagagc tgattgaggc
     2641 gcgcctgggt cacgtcgcgg cgcgtggcgg tatcgatgat gctgccgtcg atgatgaagc
     2701 tgttcttcgc atcggtatac acgagctggc cgttcttgag cacgacctcg tacagcccgg
     2761 catagtcggt acgagtcacc ctctccaccg ccggcgagcc gatgaaggcc tccatgctct
     2821 tcttgacccc ggcttcgtcg gcgtgtgcga acgtggtcgc accgagcgcc agggcgaggc
     2881 cgaggggccg gatcagggac gggacggaaa gggctttcat caagttctcc acagggttca
     2941 gaagcgaccg ttggcagcgt agcgcaccag cgcatttttt aagaccggga cccgcccggt
     3001 caggttcatc ccgagattgc gcagggtcgc cgagagggga tcctggcgcc cgaacaagcg
     3061 actcagggca tgcgtcgtgt actgcagcag gaagggctct tccgcacgca tgcgcgcata
     3121 gcgccgcagc accgaaagat cgcctgcgtc ctgccaggac ggtagcgcgt cgagcacctc
     3181 cgccagcgcc ttcgcatcct gaaagccgag gttgatgcca tgtcccgaga gcgggtggat
     3241 cgcatgcgcg gcatctccga tcagcgccac ccttgggcgc accgtctctt ccacgcgcat
     3301 cagacgcagc ggaaaagcgg cgcgctggcc ggcgagctcg agcttgccga gcgcgcggcc
     3361 gccagccccc tccacgcgcg cgcagaactc ctcggcctcg agcccgagca gctcgtcagc
     3421 gaacgcgtcg ggcgcggacc acaccatgga catgtgacgc ccgggcaggg gcagccaagc
     3481 caacacgccg tcggcgcgga accactggta agcgacaccg cgactggggc gctcgcagac
     3541 gaagttggcc accacgccgc gctcgccgta gggcgtgatc gtcgcggcga ttccggcgcg
     3601 ttcgcgtacc caggagtcgc gtccgtccgc accgacgacc aacgcggctt tcaggctgcg
     3661 accgtcgccc aggcgcaacg tcgccacatc ttcgtcgatc tcgagcgcct ccggcgccgc
     3721 cggacagaac agcgtgacgt tgtgctggcg cttgaggctc tcccagattt cgacatgaac
     3781 caagctggac tcgccgatcc aggcaagctc ctcgagtccg ctgtcgtagg ccgaaaatac
     3841 gagctcgccg cccgcatcgc cgtggatacg catctcggcg acttcagcga ggcgttggtg
     3901 gtccagatgg gcccacacgc caagctggtc caggaagcgt gcgctcaccg ggctgtaggc
     3961 gtagacgcgg gcgtcccagc cttcggggcg gcgcggcaca gcatgttcga tcacggcgat
     4021 ccgcagtgcg ctacggcgca gcgctaccgc aagcgcggcc ccggcgagac cgccgccgac
     4081 gatgatgagg tcgaatttca tcgcgatccc cggaaactcg gtacagcttt ggattgtgcc
     4141 cgagggcgcg ccttgc
//
LOCUS       DAOQBS010000195         4153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_23156_length_4153_cov_14.065154, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000195 DAOQBS010000000
VERSION     DAOQBS010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23156_length_4153_cov_14.065154"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /gene="thiC"
                     /locus_tag="PKN25_14555"
     CDS             <1..924
                     /gene="thiC"
                     /locus_tag="PKN25_14555"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase"
                     /protein_id="HNV92170.1"
                     /translation="DYFTIHAGVLLRYVPLTANRMTGIVSRGGSIMAKWCLAHHKESF
                     LYEHFEDICEIMKAYDVAFSLGDGLRPGSIYDANDEAQLGELKTLGELTDIAWKHDVQ
                     VMIEGPGHVPLQLIKENMDLQLEWCKEAPFYTLGPLTTDIAPGYDHITSGIGAATIGW
                     YGTAMLCYVTPKEHLGLPNKQDVKEGIITYKLAAHAADLAKGHPGAQIRDNALSKARF
                     EFRWEDQFNLGLDPDKAKEFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVRDFAAQ
                     QGVDEAEALQKGMEVKAVEFVKSGAEVYRNV"
     gene            1083..>4153
                     /locus_tag="PKN25_14560"
     CDS             1083..>4153
                     /locus_tag="PKN25_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV92171.1"
                     /translation="MNLLEAFTQDDLIAALGVETVAKGLGYVSRVSALSAEGDTVSAL
                     VKGRQRTPYTVEAEVEEAFGLEDGASILTSSCTCPMGYGCKHVAAMMLVWLHQRRRPD
                     RPREQVLAWVKGFRETAASLAKDETRKRKPASVAYALRYVIEPSQCGGDFGVTCHKVR
                     LDQHGDIRQREPWSNIERALQTPPAFVDETDLDLLRLLWAHRPRGHFDTDDGLPLEGR
                     HSDELMRRLISSGRAHFGSLAGRVLGLGEARSGTPDWTITSEGLRAPVLRVEPSADRV
                     LPLLPPWYIDTAKAECGPITLEVPPELVQRLLWLPPLSQAESELVAETLAEVAPSLPA
                     PTQRGEPPVELGGTPVPVLRLETRPTYHARFRQYAPHGTYQFDFALVAFRYGTVTVDA
                     GDARLFHPLPDGRSARLTRDQEAERAAAQRLAAAGFARIPPGAVQTSFGSLPADALGL
                     ASEAAWPDFMAETVPALRAEGWEVAFPTDFRHHAIDIDHLMLDIDEHEDGWLGLSPGI
                     EIDGKALPLAPLLAGLFAHDPRWLSGRLDDIDDHEAVILEDETLGRLRIGAARIKPLV
                     RALVDLFDRPDHDWRLSRLDATRLADLDLPGRGREQVAAIAARLRDAEGIRAVAAPAG
                     FRAELRPYQLEGLAWLQHLVRHDLAGILADDMGLGKTAQTLAHLLTEKQAGRLDRPAL
                     VVLPTSLVFNWQAEAERFAPDLEVLNLHGTERQARFDELDAGKKVDIALTTYPLLWRD
                     AELLQAREWSLLILDEAQTVKNAASKGAQVIRQLKARHRLGLTGTPLENHLGELWAQF
                     DFLLPGFLGSHKDFTATWRTPIEKHGDTVRRDLLAARLRPFILRRRKEDVATELPPKT
                     VIVRSVGLEGGQRDLYETVRAAMDEKVRAEIAGKGFARSQIVILDALLKLRQVCCDPR
                     LLKSPAALRVKERAKLDLLMDMLPELIDEGRRILVFSQFTTMLGLIAGELDKAKIGWV
                     ALTGDTRDRRVPVEDFQKGRVPVFLISLKAGGVGLNLTAADTVIHYDPWWNP"
BASE COUNT          663 a         1529 c         1376 g          585 t
ORIGIN      
        1 gactacttca ccatccacgc cggcgtgctg ctgcgctacg tgccgctcac cgccaaccgc
       61 atgaccggca tcgtcagccg cggcggctcg atcatggcga agtggtgcct ggcgcaccac
      121 aaggagagtt tcctctacga gcacttcgag gacatctgcg agatcatgaa ggcctacgac
      181 gtggccttca gcctcggtga cggcctgcgc ccgggctcga tctacgatgc caacgacgag
      241 gcccagctcg gcgagctgaa gaccctgggc gagctcacgg acatcgcctg gaagcacgac
      301 gtgcaggtca tgatcgaggg ccccggccac gtgccgctgc agctcatcaa ggagaacatg
      361 gacctgcagc tcgagtggtg caaggaggcg cccttctaca ccctggggcc gctcaccacc
      421 gacatcgcgc ccggctacga ccacatcacc agcggcatcg gcgcggcgac catcggctgg
      481 tacggcaccg ccatgctgtg ctacgtcacg cccaaggagc acctcggtct gcccaacaag
      541 caggacgtca aggaaggcat catcacctac aagctcgccg cgcacgccgc cgacctggcc
      601 aagggccacc cgggcgcgca gatccgcgac aacgcgctct ccaaggcgcg cttcgagttc
      661 cgctgggagg accagttcaa cctcggcctc gaccccgaca aggcgaagga attccacgac
      721 gagaccctgc ccaaggactc ggccaaggtg gcgcacttct gctcgatgtg cggcccgcac
      781 ttctgctcga tgaagatcac ccaggacgtg cgcgacttcg ccgcccagca gggtgtggac
      841 gaggccgaag cgctgcagaa gggcatggag gtgaaggcgg tggagttcgt gaagagcggg
      901 gccgaggtgt atcggaacgt gtgatgcgcg ctccgctgac gtaagtatcg acgggccgcc
      961 atcgcggccc gtttgcattg tgcgcgcgca tttttccggc ccgccgcaca cgtgcgctgc
     1021 aggctggcgt ataaactgtc gggttctccc gtttatgccc gccccgggcc tgccgcgcgc
     1081 cgatgaacct gctcgaagct ttcacccagg atgacctgat cgccgcactc ggcgtggaaa
     1141 ccgtggccaa gggcctgggc tacgtcagcc gggtcagcgc gctgtccgcc gagggcgaca
     1201 cggtgtcggc cctggtcaag ggtcgccagc gcacgcccta caccgtcgag gccgaggtgg
     1261 aagaggcgtt cggccttgaa gacggcgcct cgatcctgac cagctcgtgc acctgcccga
     1321 tgggctacgg ctgcaagcac gtcgcggcga tgatgctggt gtggctgcac cagcgccgcc
     1381 gccccgaccg cccgcgcgag caggtactag cctgggtgaa gggctttcgc gagaccgcgg
     1441 ccagcctcgc gaaagacgaa acgcgcaagc gcaagcccgc ctcggtcgcg tacgcgctgc
     1501 gctatgtgat cgaacccagc cagtgcggcg gcgacttcgg cgtcacctgc cacaaggtcc
     1561 gcctagacca gcatggagac atccgtcagc gcgagccgtg gagcaacatc gagcgcgcgc
     1621 tgcagacccc gcccgccttc gtcgacgaaa ccgacctgga cctgctgcgc ctgctgtggg
     1681 cgcatcgccc gcgcggtcat ttcgacacgg acgacggcct gccgctcgag gggcgccaca
     1741 gcgacgagct gatgcgacgg ctgatttcca gtggccgcgc gcatttcggc agtctcgccg
     1801 gccgcgtact cgggctgggc gaggcgcgct ccggcacacc cgactggacg atcacttccg
     1861 aaggcttgcg tgccccggtg ctgcgggtgg agccctccgc cgaccgcgtg ctgccgctgc
     1921 tgccgccctg gtacatcgac accgccaagg ccgagtgcgg cccgatcacg ctcgaggttc
     1981 cgcccgagct ggtgcagcgc ctgctctggc tgccgccgct cagccaggcc gaatccgagc
     2041 tggttgccga gaccctggcc gaggtggcgc ccagcctgcc cgcgcccacg caacgcggcg
     2101 agccgccggt cgaactcggc ggcacgccgg tgccggtgtt gcgcctggag acgcgtccca
     2161 cctaccacgc gcgctttcgc cagtacgcgc cccacggcac ctaccagttc gacttcgccc
     2221 tggtcgcttt ccgctacggc acggtcaccg tggatgccgg cgacgcccgc ctcttccacc
     2281 ccctgcccga cggccgcagc gcgcgcctga cccgcgacca ggaggccgag cgcgccgccg
     2341 cgcagcgcct cgccgcggcc ggcttcgccc gcatcccgcc gggtgcggtg cagaccagct
     2401 tcggcagcct gcccgccgat gcgctcgggc tggcgagcga ggcggcctgg cccgacttca
     2461 tggccgagac cgtgccggcg ctgcgcgcgg aaggctggga ggtcgccttc ccgaccgact
     2521 tccgccacca cgccatcgat atcgaccacc tgatgctcga catcgacgag cacgaggacg
     2581 gctggctcgg cctgtcgccg ggcatcgaga tcgacggcaa ggcactgccg ctggcgccgc
     2641 tgctcgccgg cctgttcgcg cacgatccgc gctggctttc cggccgcctg gacgacatcg
     2701 acgaccacga ggcggtgatc ctggaggacg agaccctggg ccgcctgcgc atcggcgcgg
     2761 cgcgcatcaa gccgctggtg cgcgcgctgg tcgacctctt cgaccgcccg gaccacgact
     2821 ggcggctatc gaggctcgac gccacccgcc tggccgacct cgacctgccc ggccgcggac
     2881 gcgagcaggt cgccgcgatc gccgcgcgcc tgcgcgacgc cgagggcatc cgcgccgtcg
     2941 ccgcaccggc gggcttccgc gccgagctgc gcccctacca gctcgaaggc ctggcctggc
     3001 tgcagcatct cgtacgccac gacctcgccg gcatcctggc cgacgacatg gggctgggca
     3061 agaccgcaca gacgctcgcc cacctgctca ccgagaagca ggccggccgg ctcgaccgcc
     3121 ccgccctggt ggtgctgccg acctcgctgg tgttcaactg gcaagccgag gccgagcgct
     3181 tcgcgcccga cctcgaggtc ctcaacctgc acggcaccga acggcaggcg cgcttcgacg
     3241 agctggacgc cggcaagaag gtcgacatcg cgctcaccac ctatccgctg ctgtggcgcg
     3301 acgccgagct gctgcaggcg cgcgaatgga gcctgctgat cctcgacgag gcgcagacgg
     3361 tgaagaacgc cgccagcaag ggcgcgcagg tgatccgcca gctcaaggcc cgccaccgcc
     3421 tcggcctcac cggcacgccg ctggagaacc acctcggcga gctatgggcg cagttcgact
     3481 tcctgttgcc gggcttcctg ggcagccaca aggacttcac cgcgacctgg cgcacgccga
     3541 tcgaaaagca cggcgacacg gttcgccgcg atctgctcgc ggcacgcctg cgccccttca
     3601 tcctgcgccg gcgcaaggag gacgtggcca ccgaactccc gcccaagacc gtgatcgtgc
     3661 gcagcgtcgg cctggagggc ggccagcgcg acctctacga gacggtgcgc gcggcgatgg
     3721 acgagaaggt gcgcgcggag atcgccggca agggcttcgc gcgcagccag atcgtgatcc
     3781 tcgacgcgct gttgaagctg cgccaggtgt gctgcgaccc gcgcctgttg aagtcgcccg
     3841 ccgccttgcg ggtgaaggag cgcgccaagc tcgacctgct catggacatg ctccccgagc
     3901 tgatcgacga gggccggcgc atcctggtgt tctcgcagtt caccaccatg ctcggcctga
     3961 tcgctggcga actcgacaag gcgaagatcg gctgggtcgc gctgaccggc gacacccgcg
     4021 atcgccgggt gccggtggaa gacttccaga aggggcgcgt gccggtattc ctgatcagcc
     4081 tgaaggcggg cggcgtgggc ctcaacctca ccgccgccga caccgtgatc cactacgacc
     4141 cgtggtggaa ccc
//
LOCUS       DAOQBS010000196         4131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_23347_length_4131_cov_14.243621, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000196 DAOQBS010000000
VERSION     DAOQBS010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23347_length_4131_cov_14.243621"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /gene="flhF"
                     /locus_tag="PKN25_14565"
     CDS             <1..639
                     /gene="flhF"
                     /locus_tag="PKN25_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585750.1"
                     /note="positive regulator of class III flagellar genes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNV92172.1"
                     /translation="ALVGPTGVGKTTTTAKLAARCVVRHGAGRLALITTDGYRIGAQE
                     QLRIYGRILGVPVFSVRDAADLRQTLAGLRNKHMVLIDTMGMSQRDRMVAEQAAMLSG
                     AGDVRRLLLLNATARGDTLDDVVRAYAGGDLAGCIFTKVDEAASLAPALDVAVRHELD
                     IRYLTNGQRVPEDLHLPNRAYLLHRALREHGSASPWHLQGDEAGMLLAAGGA"
     gene            642..1427
                     /locus_tag="PKN25_14570"
     CDS             642..1427
                     /locus_tag="PKN25_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FleN"
                     /protein_id="HNV92173.1"
                     /translation="MLDGREDQAAGLRRLFRRAPPQVVALYAGGRRAADNAALAAHRI
                     AGRSERVLVLDEAEGGARLCGALGVGEGVDLLQLLDGRSTLADIVQPVPGLVGRVPAA
                     AAALALPLLDDPRRACLIEALRILHRHAGFVLVHASGEQAAEPSPFVLAAPRRLVVAE
                     ASASGATDAYHTIKGLAAAGAGSVHVAVCRARGRADATAFFRSLDALVRRHVGVPLAW
                     VGELERDEIAGGLQQPLQASTHDAGAAFLRRVGAMARAGVMRA"
     gene            1552..2325
                     /locus_tag="PKN25_14575"
     CDS             1552..2325
                     /locus_tag="PKN25_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HNV92174.1"
                     /translation="MYDAHGKLDKGGLVENYAPLVKRIAYQLMSRLPASVDVDDLIQN
                     GMMGLLDALGRYEQGLGAQFETYAVQRIRGAMLDGLRENDWLPRSLRRDMRRIEAAIH
                     SLEQQNGRPPSESELAAALDLPLEDYQRMLQDARGHQLVYLEDFNREEDDDYLDRHLA
                     QEGNDPLTLLEDTDLRRRLVGAIEHLPEREQLVMALYYDEELNLREIGEVLGVTESRV
                     CQLHSQAVARLRACVFEGGTAMPVARRRGRPPKNAAAGR"
     gene            2356..3096
                     /locus_tag="PKN25_14580"
     CDS             2356..3096
                     /locus_tag="PKN25_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNV92175.1"
                     /translation="MDKISLVGLTLGIAAILVGQVIEGGHLSSLFQPTAFMIVIGGTL
                     GAVMLQSPLRVFMQGMRMARWVFVPPAPAFDTIIEQVANWGQTARKEGLLSLENQIEA
                     LSDPFMRKGLQLLVDGIEPNRLREVLEVEIGVWENQMKMSARIWEAAGGYAPTIGILG
                     AVMGLIHVMENLSDPSRLGAGIAVAFVATIYGVGFANLVFLPIAKKLGAHIVTLTTQR
                     EMFVDGLVGIANGDNPRIIASRMQGYVA"
     gene            3112..3951
                     /gene="motD"
                     /locus_tag="PKN25_14585"
     CDS             3112..3951
                     /gene="motD"
                     /locus_tag="PKN25_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HNV92176.1"
                     /translation="MRRRRKLDEEHDNHERWLVSYADFITLLFAFFVVMYSLSSVNEG
                     KYRVLSDSLVQAFRNVNVNESGEQIVLPKVSVVPSQAITRPAPPAVDPVVEQRRQQAA
                     QRMRNVADEIRRVLSPLTGSGQVKVTEGAFGVSVEINASLLFAPGEAALGSNAVSALQ
                     AVARVLAPAEFPITVEGHTDNVPISTFRFPSNWELSAVRASTVARLFIDNGVTPARLT
                     AAGYADQRPVADNADEEGRGRNRRVTILIESRMADPEGNAGPTTIPPDDPIRSILPEA
                     PQG"
BASE COUNT          597 a         1515 c         1426 g          593 t
ORIGIN      
        1 gccctggtcg gccccaccgg cgtcggcaag accaccacca ccgccaagct cgccgcccgc
       61 tgcgtggtgc gccacggcgc cggccggctc gcgctgatca ccaccgacgg ctaccgcatc
      121 ggcgcccagg agcagctgcg catctacggc cgtatcctcg gcgtgccggt gttctcggtg
      181 cgcgacgcgg ccgacctgcg ccagaccctg gccggcctgc gcaacaagca catggtgctc
      241 atcgacacca tgggcatgag ccagcgcgac cgcatggtgg ccgagcaggc ggccatgctc
      301 tccggcgccg gcgacgtgcg ccggctgctg ctgctcaacg ccaccgcgcg cggcgacacc
      361 ctcgacgacg tggtgcgcgc ctatgccggc ggcgacctcg ccggctgcat cttcaccaag
      421 gtggacgagg ccgcctcgct cgcgccggcg ctcgatgtcg cggtgcgcca cgagctcgac
      481 atccgctacc tcaccaacgg ccagcgcgta cccgaggacc tgcacctgcc caaccgtgcc
      541 tacctcttgc atcgcgcgct gcgcgagcac ggcagcgcct cgccctggca cctgcagggc
      601 gacgaggccg gcatgctgct cgcggcggga ggggcctgag catgctcgac ggacgcgaag
      661 accaggccgc cggcctgcgc aggctgttcc gccgcgcgcc gccccaggtg gtggcgctgt
      721 atgcgggcgg tcgccgcgcc gccgacaacg ccgcgctcgc cgcccaccgc atcgccgggc
      781 gcagcgagcg tgtgctggtg ctggacgagg ccgagggcgg cgccaggctg tgcggcgcgc
      841 tcggcgtggg cgagggcgtg gacctgctgc agctgctcga cggccgcagc acgctcgccg
      901 acatcgtgca gccggtgccg ggcctggtgg gccgcgtgcc ggccgcggcc gcggcgctcg
      961 cgctgcccct gctcgacgat ccgcgccgtg cctgcctgat cgaggcgctg cgcatcctgc
     1021 accgccacgc cggcttcgtt ctggtgcacg cgagcggcga acaggctgcg gagccgtctc
     1081 cgttcgtgct cgccgcgccg cgccgactgg tggtcgccga ggcgagcgcg agcggcgcca
     1141 ccgacgccta ccacaccatc aagggccttg ccgccgcggg agccggctcg gtgcacgtgg
     1201 ccgtgtgccg cgcgcgcggg cgggcggacg ccaccgcctt cttccgcagt ctcgatgcgc
     1261 tcgtgcgccg ccacgtgggc gtgccgctcg cgtgggtggg cgagctcgaa cgcgacgaga
     1321 tcgccggcgg cctgcagcag ccgctgcagg cttcgaccca cgatgcgggc gcggccttcc
     1381 tgcgccgggt cggcgccatg gcgcgtgccg gcgtcatgcg cgcctgatgc gccgccgacg
     1441 tgacagatcg cgctggtaaa ggcggcgatg ctacgggaaa atgcacaact cctatccacg
     1501 cgggcggggt ccgacggtcc cgtctgctgc cagaacccac aggcccgatg catgtacgac
     1561 gcacacggta agctcgacaa gggtggcctc gtcgagaact acgccccgct ggtcaagcgc
     1621 atcgcctacc agttgatgtc gcgcctgccc gccagcgtgg atgtggacga cctgatccag
     1681 aacggcatga tgggcctgct cgatgccctc ggccgctacg agcagggcct cggcgcccag
     1741 ttcgagacct atgcggtgca gcgcatccgc ggcgccatgc tcgacggcct gcgcgagaac
     1801 gactggctgc cgcgcagcct gcgccgcgac atgcgccgca tcgaggcggc gatccacagc
     1861 ctggagcagc agaacggccg cccgccctcc gagagcgagc tcgccgccgc gctcgacctg
     1921 ccgctggagg attaccagcg catgctgcag gacgcgcgcg gccaccagct cgtctatctc
     1981 gaggatttca accgcgagga agacgacgac tatctcgacc gccatctcgc ccaggagggc
     2041 aacgatccgc tcaccctgct cgaggacacc gacctgcgcc gccgcctggt cggggcgatc
     2101 gagcacctgc ccgagcgcga gcagctcgtg atggcgctgt attacgacga ggagctcaac
     2161 ctgcgcgaga tcggcgaggt actcggcgtc accgagtcgc gcgtgtgcca gctgcacagc
     2221 caggcggtgg ctcgcctgcg cgcctgcgtc ttcgagggcg gcacggcgat gccggtcgcg
     2281 cgccggcgcg ggcgcccacc caagaacgcg gccgcgggcc gctgatcggg gccgccagcg
     2341 ctgcccccgg ataccatgga caagatcagc ctcgtcggcc tcaccctcgg aatcgccgcc
     2401 atcctcgtcg gccaggtgat cgagggcggt cacctgtcct cgctgttcca gcccaccgcg
     2461 ttcatgatcg tgatcggcgg cacgctcggc gcggtcatgc tgcagagccc gctgcgcgtc
     2521 ttcatgcagg gcatgcgcat ggcgcgctgg gtcttcgtgc cgccggcgcc cgccttcgat
     2581 acgatcatcg agcaggtcgc gaactggggc cagaccgcgc gcaaggaggg cctgctgagc
     2641 ctggagaacc agatcgaggc gctgtccgac cccttcatgc gcaagggcct gcagctgctg
     2701 gtcgacggca tcgagcccaa ccgcctgcgc gaggtgctcg aggtcgagat cggcgtgtgg
     2761 gagaaccaga tgaagatgtc ggcgcgcatc tgggaggcgg ccggcggcta tgcgccgacg
     2821 atcggcatcc tgggcgcggt gatgggcctg atccacgtga tggagaacct ctccgaccct
     2881 tcgcggctgg gcgccggcat cgcggtcgcc ttcgtcgcca cgatctacgg cgtgggcttc
     2941 gccaacctgg tgttcctgcc gatcgccaag aagctgggcg cgcacatcgt caccctgacc
     3001 acccagcgcg agatgttcgt cgatggtctg gtggggatcg ccaacggcga caacccgcgc
     3061 atcatcgcca gccgcatgca gggctacgtg gcctgagcgc cgggagacat catgcggcgc
     3121 aggcgcaagc tcgacgagga acacgacaac cacgaacgct ggctggtgtc ctacgccgac
     3181 ttcatcacgc tgctgttcgc cttcttcgtg gtcatgtatt cgctgtcgtc ggtcaacgag
     3241 ggcaagtacc gcgtgctctc cgactcgctc gtgcaggcct tccgcaacgt caatgtgaac
     3301 gagagcggcg aacagatcgt gctgcccaag gtcagcgtcg tgccttcgca ggcgatcacc
     3361 cggccggcgc ccccggcggt ggatcctgtc gtggagcagc gtcgccagca ggccgcgcag
     3421 cgcatgcgca acgtggccga cgagatccgc cgcgtgctct cgccgctgac ggggtccggc
     3481 caggtgaagg tcaccgaggg cgccttcggc gtcagcgtcg agatcaacgc cagcctgctc
     3541 ttcgcgccgg gcgaggccgc gctcggcagc aatgccgtgt cggcgctgca ggcggtggcg
     3601 cgggtgctgg cgccggcgga gttcccgatc acggtcgagg gccacaccga caacgtgccg
     3661 atcagcacct tccgctttcc gtcgaactgg gagctctcgg cggtgcgtgc ctcgacggtg
     3721 gcgcggctct tcatcgacaa cggcgtcacc ccggcgcgcc tgaccgccgc cggctatgcc
     3781 gaccagcgcc cggtcgcgga caacgcggac gaggagggcc gcggccgcaa ccgtcgcgtc
     3841 accatcctga tcgagtcgcg catggccgac cccgaaggca atgccggccc gaccaccatc
     3901 ccgcccgacg accccatccg ctccatcctg ccggaggccc cgcagggttg atggcagccg
     3961 gtcggggtga gaccccacgg cgtgtccggt tccttgcgga tcttgtagca gcgacgcaag
     4021 tcgcgatcgg cgcaacgatt cccgccgtgc ctttttcgcc gctgcgatcg cgacttgcgt
     4081 cgctcccacg agagacgccg aggtgcattg gtacgtcgct cccacgagag a
//
LOCUS       DAOQBS010000197         4073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_23803_length_4073_cov_16.061722, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000197 DAOQBS010000000
VERSION     DAOQBS010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23803_length_4073_cov_16.061722"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..431)
                     /locus_tag="PKN25_14590"
     CDS             complement(90..431)
                     /locus_tag="PKN25_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNV92177.1"
                     /translation="MSSLPRCPACNSEYTYEDGDNFVCPECAHEWPKLAAAEATEAHK
                     VWKDANGNVLQDGDSVTVIKDLKVKGSSLVVKVGTKVKNIRLVEGDHDIDCKIDGIGA
                     MKLKSEFVKKN"
     gene            complement(487..1218)
                     /locus_tag="PKN25_14595"
     CDS             complement(487..1218)
                     /locus_tag="PKN25_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV92178.1"
                     /translation="MHKDLPDLRTPSRTPVHGLRRLVLAAAFVAGGLASHAALAATLD
                     DALSAASMGDAKELVSLIQRGLDPDTVDAQGNTLLILAAREGHTAAVEALAKNRARLD
                     YRNPAGDSALMLAVLRGHEDAARALIKAGAVVAHDGWAPLHYAAFEGRDELIADILAA
                     GADVNAPAPNKATPLMLAARNGHIDVVRRLLALPQTDLNALNDAGLSADAWALQNNNT
                     DIAELIQAERKRRGIRPPAMKITIE"
     gene            complement(1199..1975)
                     /locus_tag="PKN25_14600"
     CDS             complement(1199..1975)
                     /locus_tag="PKN25_14600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNV92179.1"
                     /translation="MFVDSHCHLDFPGLAEREDEILATMAANGVGTALCIGVRLEEFP
                     RVRELAERHAHLWASVGVHPDNADCEEPDVARLVALADHPRVVAIGETGLDYYWHKDA
                     PAWQRERFRVHIRAARATGKPLVVHTRNAAADTLRLMREEGAGEAGGVMHCFTESWEV
                     AEAALELGFYISFSGIVSFRNAKELKEVARRVPLERLLIETDSPYLAPVPHRGKTNEP
                     GWVVHVAEEIARLRELPLAQVEQATTDNFFRLFRHAQRPA"
     gene            complement(2062..2427)
                     /locus_tag="PKN25_14605"
     CDS             complement(2062..2427)
                     /locus_tag="PKN25_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV92180.1"
                     /translation="MTQAARPAVSRPSVLSLNISSKSALYAAYMPFLANGGIFVPTPK
                     SYKLGDEVFMLLQLMDDATRHPVAGTVVWITPDGAQGGKTQGIGVQFSPDESSRVLRD
                     RIEKVLAGHLGSSRPTHTL"
     gene            complement(2424..3467)
                     /gene="holB"
                     /locus_tag="PKN25_14610"
     CDS             complement(2424..3467)
                     /gene="holB"
                     /locus_tag="PKN25_14610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNV92181.1"
                     /translation="MIHPWLQSTWTRLVELGERLPHALLFVGPAGLGKRALAEALAAR
                     LLCDAPGADGHACGQCEPCLWRLSGNHPDLHRVVPEAETAAAEGEGDGEAPGKAASTQ
                     IRIDQIRALQGALAVTGHHGSRRVVLLDPAEAMNGVTANALLKLLEEPPEGCIFVLVS
                     SAPRILLPTIRSRCQQWHFSRPDAAALARWGAHAAADARALLALGGGMPLAAERLAQG
                     GGAAMHARFVRDIAGLQGADALRLAGQWEAWLRSKDALAAGFGLPELTDWMQRWVSDL
                     AALRLGGRVRFFPAQEGVLAALASRMSVAAASACYNEVAQIRKVSRHPLSLRLVLEDM
                     LMRYARAVAGARG"
     gene            complement(3464..>4073)
                     /gene="tmk"
                     /locus_tag="PKN25_14615"
     CDS             complement(3464..>4073)
                     /gene="tmk"
                     /locus_tag="PKN25_14615"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNV92182.1"
                     /translation="RFITFEGIDGAGKSSQIAAVVALLQAAGRDVDQTREPGGTALGE
                     RLRELLLHEPMHLETEAMLMFAARREHLAARIEPALAAGRWVVCDRFSDATFAYQVGG
                     RGLDRAKFDALEAWVHPGLQPDLTLVFDLDPALAAARVASTGVDPDRFEREQRDFFER
                     VRAAYLERARLAPLRICVIDAARSPEAIRADIERVVRERFFS"
BASE COUNT          565 a         1374 c         1525 g          609 t
ORIGIN      
        1 cccacgcccc tcaccaccgt gccgtgcgat cgcgacttgc gtcgctccta caagaccggc
       61 gcggttgcgg agcgtttcct gacgaccgct caattcttct tcacgaactc cgacttcagc
      121 ttcatcgcgc cgatgccgtc gatcttgcag tcgatgtcgt ggtcgccctc gaccaggcgg
      181 atgttcttca ccttggtgcc gaccttgacg accagggacg agcccttgac cttgaggtcc
      241 ttgatcacgg tgacgctgtc gccgtcctga agcacgtttc cgttggcgtc cttccacacc
      301 ttgtgcgcct cggtggcctc ggctgcggcg agcttcggcc attcgtgggc gcattcgggg
      361 cagacgaagt tgtcgccgtc ctcgtaggtg tattcggaat tgcaggcggg gcacctgggc
      421 aggctgctca tttcgtttcc tgatggattg gagcgcaggc ggcgcgacgg ccgcccggat
      481 ggggaatcat tcgatcgtga tcttcatcgc cggcggacgg atgccgcggc gcttgcgctc
      541 ggcctggatc agttcggcga tgtcggtgtt gttgttctgc agcgcccagg cgtcggccga
      601 aaggccggcg tcgttgagcg cgttgagatc ggtctgtggc agcgcgagca ggcggcggac
      661 cacgtcgatg tggccgttgc gcgccgccag catcagcggc gtggccttgt tgggcgcggg
      721 tgcattgacg tcggcgccgg cggcgaggat gtccgcgatc agctcgtcgc ggccctcgaa
      781 ggcggcgtag tgcagcggcg cccagccgtc gtgcgcaacc acggcgccgg ccttgatgag
      841 cgcgcgtgcg gcgtcctcgt ggccgcgcag cacggccagc atcagcgcgg agtcgcccgc
      901 cgggttgcgg tagtcgaggc gggcgcggtt cttcgccagc gcctccacgg ccgcggtgtg
      961 gccctcgcgc gcggcgagga tgagcaaggt gttgccctgc gcgtcgacgg tatcggggtc
     1021 gaggccgcgc tggatcaggc tgacgagttc cttcgcgtcg cccatgctgg cggcggagag
     1081 cgcgtcgtcg agggtggcgg cgagcgcggc atgcgaagcc aggccgccgg cgacgaaggc
     1141 cgcggcgagg acgaggcggc gcagcccatg taccggagtg cgggagggcg tgcggagatc
     1201 aggcaggtct ttgtgcatgg cggaagagac ggaagaagtt gtcggtggtg gcttgctcga
     1261 cctgcgccag cgggagctcg cgcaggcggg cgatctcctc ggcgacatgg acgacccagc
     1321 cgggctcgtt ggtcttgccg cggtgcggca ccggggcgag atagggggaa tcggtctcga
     1381 tcagcaggcg ctcgagcggc acgcggcggg cgacctcctt gagctccttc gcgttgcgaa
     1441 agctcacgat gcccgagaac gagatgtaga agccgagctc gagcgcagcc tcggccacct
     1501 cccagctctc ggtgaagcag tgcatcactc cgccggcctc gccggccccc tcctcgcgca
     1561 tcaggcgcag ggtgtccgcg gcggcgttgc gggtgtggac gaccagcggc ttgcccgtcg
     1621 cacgcgccgc gcggatgtgc acccgaaagc gctcccgctg ccacgcgggc gcgtccttgt
     1681 gccagtaata gtccagcccg gtctcgccga tcgccaccac gcgcgggtgg tccgcgagcg
     1741 cgacgaggcg ggcgacgtcc ggctcctcgc aatcggcatt gtccggatgc acgccgaccg
     1801 aggcccacag gtgggcgtgg cgctcggcga gctcacgcac acgcgggaat tcctccagcc
     1861 gtacgccgat gcacagcgcg gtgccgacgc cgttggccgc catggtggcg aggatctcgt
     1921 cctcgcgctc ggcgaggccg ggaaaatcga gatgacagtg tgaatcgacg aacatccggg
     1981 tcctggaatg gtttgatgaa gtcggcgttg ttgaactcgg cagcgcgcga cgcgaagcgg
     2041 gccctgccct tgccggcgcg ctcagagcgt gtgcgtgggc cggctcgagc cgagatggcc
     2101 ggcgagcacc ttctcgatgc ggtcgcgcag cacccggctg gactcgtcgg gcgagaactg
     2161 cacgccgatg ccctgggtct tgccgccctg cgcgccgtcc ggcgtgatcc acaccacggt
     2221 gcccgcgacc ggatggcggg tggcgtcgtc catgagctgc agcagcatga agacctcgtc
     2281 gcccagcttg tagctcttgg gcgtgggcac gaagatcccg ccgttggcca ggaagggcat
     2341 gtaggccgcg tagagcgccg acttggagct gatgttgagc gacagcacgc tcgggcggct
     2401 gaccgctggt cgagcggctt gggtcatcct cgggctccgg cgacggcacg ggcgtagcgc
     2461 atcagcatgt cctccagcac cagccgcagg ctcagcggat ggcgcgacac cttgcggatc
     2521 tgggcgactt cattgtagca ggcgctcgcc gcggccacgc tcatgcggct ggccagcgcg
     2581 gcgagcacgc cctcctgcgc ggggaagaag cgcacccggc cacccaggcg cagcgcggcg
     2641 aggtcgctca cccagcgctg catccagtcg gtgagctcgg gcaggccgaa gccggcggcg
     2701 agcgcatcct tcgagcgcag ccaggcctcc cactgcccgg ccaggcgcag cgcgtcggcg
     2761 ccttgcaggc cggcgatatc gcgcacgaag cgcgcgtgca tcgccgcgcc gccaccctgc
     2821 gcgaggcgct cggcggcgag cggcatgccg ccgcccagcg ccagcagcgc acgcgcatcg
     2881 gcggcggcat gcgcgcccca gcgcgccagc gcagctgcgt cgggacggct gaaatgccac
     2941 tgctggcagc ggctgcggat ggtgggcagc aggatgcgcg gcgcggagga cacgagcacg
     3001 aagatgcagc cctctggtgg ctcctcaagc agcttgagca gcgcgttggc ggtgacgccg
     3061 ttcatcgcct cggccggatc gagcagcacg acgcggcgcg agccatggtg gccggtgacg
     3121 gcgagcgcgc cctgcagcgc gcggatctgg tcgatgcgga tctgcgtgga ggccgccttg
     3181 ccgggcgcct cgccgtcgcc ctcgccctcc gccgcggcgg tctcggcctc gggcacgact
     3241 ctgtgcaggt cggggtggtt gcccgagagc cgccacaggc agggctcgca ttgcccgcag
     3301 gcgtggccgt cggcgccggg ggcgtcgcac agcaggcgcg ccgccagcgc ctcggcgagc
     3361 gcgcgcttgc ccaggcctgc ggggccgacg aagagcagcg cgtgcggcag gcgttcgccc
     3421 agctcgacga gccgcgtcca cgtcgattgc agccaggggt ggatcatgag aagaagcgct
     3481 cgcgcacgac gcgctcgatg tcggcgcgga tcgcctccgg cgagcgcgcg gcatcgatca
     3541 cgcaaatgcg cagcggcgcc aggcgggcgc gttcgaggta ggcggcgcgc acgcgctcga
     3601 agaagtcgcg ctgctcgcgc tcgaagcggt cggggtcgac cccggtggag gcgacgcgcg
     3661 cggcggcgag cgccggatcg aggtcgaaca ccagggtgag gtcgggctgc aggccgggat
     3721 gcacccaggc ctcgagcgcg tcgaacttgg cgcggtcgag cccgcgcccc cccacctgat
     3781 aggcgaaggt ggcgtcggag aagcggtcgc acaccaccca gcgcccggcg gcgagcgcgg
     3841 gctcgatgcg cgcggcgagg tgctcgcggc gcgcggcgaa catcagcatg gcctcggtct
     3901 cgaggtgcat cggctcgtgc agcagcagct cgcgcaggcg ttctccgagc gccgtgccgc
     3961 cgggctcgcg ggtctggtcg acgtcgcggc ctgcggcctg cagcaacgcc accacggcgg
     4021 cgatctggct gctcttgccg gcaccgtcga tgccttcgaa ggtgatgaag cgg
//
LOCUS       DAOQBS010000198         4064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_23875_length_4064_cov_13.085308, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000198 DAOQBS010000000
VERSION     DAOQBS010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23875_length_4064_cov_13.085308"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PKN25_14620"
     CDS             complement(<1..644)
                     /locus_tag="PKN25_14620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343151.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNV92183.1"
                     /translation="MKSLLADLFKQTLPNEEEFDAITIGLASPDKIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTLSKVRRE
                     RMGHIELASPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVVEPGMTPLNRGQ
                     LLTEDDYLAKVEEYGDEFDAVMGAEGIGSLLRTLDINLEAEKLRGDLETTNSEA"
     gene            complement(730..>4064)
                     /gene="rpoB"
                     /locus_tag="PKN25_14625"
     CDS             complement(730..>4064)
                     /gene="rpoB"
                     /locus_tag="PKN25_14625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNV92184.1"
                     /translation="VAREKRITSKHIREIDQAGVKTMAVPDDFLLGRVIATNVVDPAT
                     GELLARANDEITEDLLDKLRLAGVTGLSTLYINDLDRGGYISQTLRIDETADQWAAKV
                     AIYRMMRPGEPPTEDAVEGLFQGLFYAEERYDLSSVGRMKFNRRAYPEKIDDKAPGWL
                     KRFYQRVGAQGEDGPGVLVNDDILAVVGVLVELRNGRGEIDDIDHLGNRRVRSVGELA
                     ENQFRAGLVRVERAVKERLSQAESDNLMPHDLINAKPISAAIKEFFGSSQLSQFMDQT
                     NPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLA
                     VYARTNRHGFLETPYRKVNDGKVTDQIDFLSAIEEGQYVIAQANAEIDEAGMLAGDLV
                     SCRHKGEFLLATSDMVQYMDVAPGQIVSVAASLIPFLEHDDANRALMGANMQRQAVPC
                     LRPEKPLVGTGIERRVAVDSGTAVQATRGGIVDYVDANRVVIRVNDDETLPGEVGVDI
                     YNMIKYTRSNQNTNINQRPVVKVGDLIARGDVVADGASTDLGELALGQNMLVGFMPWN
                     GYNFEDSILISERVVADDRFTSIHIEELTVVARDTKLGPEEITRDIASLGEAQLSRLD
                     ESGIVYIGAEVEAGDVLVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDTSLRVPSGM
                     VGTVIDVQVFTREGIERDKRAQSIIDEQLRSFKTDLADQMRIVERDAFARIRRMIVGQ
                     LANGGPKKLAKGAALTDPYLDSLEHYHWFDIRLADETLAVQLEAVREGLENTRKDFEQ
                     AFEIKKKKLTQGDELPPGVQKMVKVYLAVKRRLQPGDKMAGRHGNKGVVSKIVPVEDM
                     PYMEDGTPLDIVLNPLGVPSRMNIGQILETHLGWAAKGLGQKIDKMLQAQASALELRG
                     FLEQIYNGSGHPEDIANLGDSDVRELAGNLKKGVPFATPVFDGAKEEEIQQMLELAGL
                     PTGGQVTLYDGRTGEAFERKVTVGYKHVLKLHHLVDDKMHARSTGPYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVTGRTKVYENIVKGEHKIDSGMP
                     ESFNVLVKEIRSLAIDIDLDRE"
BASE COUNT          610 a         1331 c         1359 g          764 t
ORIGIN      
        1 ttggcttcgg agttggtggt ctccaggtcg ccacgcagct tctcggcctc gaggttgatg
       61 tcgagcgtgc gcagcaggga accgataccc tcggcaccca tgaccgcgtc gaactcgtca
      121 ccgtactcct cgaccttggc gagatagtcg tcctcggtca gcagctggcc gcggttgagc
      181 ggcgtcatgc ccggctcgac gacgacgaag gcttcgaagt agagcacgcg ctcgatatcg
      241 cgcagggtca tgtcgagaac catgccgaga cggctcggca agctcttcag gaaccagatg
      301 tgggcgaccg gcgaggccag ctcgatgtgg cccatgcgct cgcgccgcac cttcgacagc
      361 gtcacctcga cgccgcactt ctcgcagatc acgccacggt gcttgaggcg cttgtacttg
      421 ccgcacaggc actcgtagtc cttgaccggc ccgaagatct tggcgcagaa caggccgtca
      481 cgctcgggtt tgaacgtgcg gtagttgatc gtctcgggct tcttgacttc gccataggac
      541 cacgagcgga tcttgtccgg cgaggcgagg ccgatcgtga tcgcgtcgaa ctcttcctcg
      601 ttcggcaggg tttgcttgaa caggtcagca agcaggcttt tcatggttca ctccatccgg
      661 gccgccgcgt cgtgggcggc ggccccggga ttcaatcgca ggcaggggac gggaaggaaa
      721 tcgcgacgct cactcgcggt cgaggtcgat gtcgatcgcc agcgaacgga tttccttcac
      781 cagcacgttg aaggattccg gcatgccgga atcgatcttg tgctcgccct tgacgatgtt
      841 ttcgtacacc ttggtccggc cggtcacgtc gtcggacttg accgtcagca tctcctgcag
      901 cgtgtaggcg gcgccgtagg cttccagcgc ccacacttcc atctcgccga agcgctggcc
      961 gccgaactgc gccttgccgc ccagcggctg ctgggtgacg agcgagtacg ggccggtcga
     1021 acgcgcgtgc atcttgtcgt cgaccaggtg gtgcagcttc agcacatgct tgtagcccac
     1081 ggtgaccttg cgctcgaagg cctcgccggt gcggccgtcg tagagggtga cctggccgcc
     1141 ggtgggcagg ccggccagct cgagcatctg ctggatttcc tcttccttgg caccgtcgaa
     1201 caccggggtc gcgaagggca cgcccttctt gaggttgccc gcgagctcgc gaacgtccga
     1261 gtcgccgagg ttggcgatgt cctcggggtg gccgctgccg ttgtagatct gctcgaggaa
     1321 gccgcgcagc tccagcgccg aggcctgcgc ctgcagcatc ttgtcgatct tctgaccgag
     1381 tcccttcgct gcccagccga ggtgggtctc gaggatctgg ccgatgttca tccgcgacgg
     1441 cacgccaagc gggttgagca cgatgtcgag cggggtgccg tcttccatgt acggcatgtc
     1501 ctcgaccggg acgatcttgg aaaccacgcc cttgttgccg tgacggccgg ccatcttgtc
     1561 gcccggctgc aggcggcgct tcaccgccag atacaccttg accatcttct gcacgcccgg
     1621 ggggagctcg tcgccctggg tgagcttctt cttcttgatc tcgaaggcct gctcgaagtc
     1681 cttgcgggtg ttctccagac cttcacgcac ggcctcgagc tgcaccgcga gggtctcgtc
     1741 ggcgaggcgg atgtcgaacc agtggtagtg ctccaggctg tcgaggtagg gatcggtgag
     1801 cgccgcaccc ttggcgagct tcttcggacc accgttggca agctggccca cgatcatgcg
     1861 gcggatacgc gcgaaggcgt cgcgctcgac gatgcgcatc tggtcggcga ggtcggtctt
     1921 gaagctgcgc agctgctcgt cgatgatcga ctgcgcgcgc ttgtcgcgct cgatgccctc
     1981 gcgggtgaac acctgcacgt cgatgacggt gccgaccatg cccgagggca cgcgcagcga
     2041 ggtgtccttc acgtcggagg ccttctcgcc gaagatcgcg cgcagcagct tctcctcggg
     2101 ggtgagctgg gtctcgccct tgggcgtgac cttgccgacc agcacgtcgc cggcctcgac
     2161 ctcggcaccg atatacacga tgcccgactc gtccaggcgc gacagctgcg cctcgccgag
     2221 cgaggcgatg tcgcgggtga tctcctcggg tccgagcttg gtgtcgcggg cgacgacggt
     2281 gagttcctcg atgtggatcg aggtgaagcg gtcgtcggcg accacgcgct cggagatgag
     2341 gatcgagtcc tcgaagttgt agccgttcca gggcatgaag ccgaccagca tgttctggcc
     2401 gagcgcgagc tcgccgaggt cggtggaagc gccgtcggcc accacgtcac cgcgggcgat
     2461 caggtcgccc accttcacca ccgggcgctg gttgatgttg gtgttctggt tggaacgggt
     2521 gtacttgatc atgttgtaga tgtcgacgcc gacttcaccc ggcagggtct cgtcgtcgtt
     2581 cacccggatc accacccggt tcgcatccac gtagtccacg atgccgccac gggtggcctg
     2641 caccgcagtg cccgagtcga ccgccacgcg gcgctcgatg ccggtgccga ccaggggctt
     2701 ttccgggcgc aggcagggca ccgcctggcg ctgcatgttg gcgcccatca gcgcgcggtt
     2761 ggcgtcgtcg tgctccagga agggaatcag cgaagccgcg accgacacga tctggcccgg
     2821 ggccacgtcc atgtactgca ccatgtccga ggtggcgagc aggaactcgc ccttgtggcg
     2881 gcacgacacc aggtcgccgg cgagcatgcc cgcctcgtcg atctcggcgt tcgcctgcgc
     2941 gatcacatac tgcccctcct cgatcgccga caggaagtcg atctggtcgg tgaccttgcc
     3001 gtcattgacc ttgcggtacg gcgtctcgag gaagccgtgg cggttggtgc gcgcatacac
     3061 cgcgagcgag ttgatcaggc cgatgttcgg accttccggg gtctcgatcg ggcacacgcg
     3121 gccgtagtgc gtcgggtgca cgtcgcgcac ctcgaagccg gcgcgctcac gcgtcaggcc
     3181 gcccgggccc agtgccgaga cccgacgctt gtgcgtgatc tcggacagcg ggttggtctg
     3241 atccatgaac tgcgagagct ggctggagcc gaagaactcc ttgatcgccg ccgagatcgg
     3301 cttggcgttg atcaggtcgt gcggcatcag gttgtcggac tcggcctgcg acaggcgctc
     3361 cttgaccgcg cgctcgacgc gcaccaggcc cgcacggaac tggttctcgg cgagctcgcc
     3421 caccgaacgc acgcggcggt tgccgaggtg gtcgatgtcg tcgatctcgc cgcggccatt
     3481 gcgcagctcg accagcacgc ccaccaccgc gaggatgtcg tcattgacca gcacgcccgg
     3541 gccgtcctcg ccctgcgcgc cgacgcgctg gtagaagcgc ttgagccagc ccggcgcctt
     3601 gtcgtcgatc ttctcggggt aggcgcggcg attgaacttc atgcggccga ccgaggacag
     3661 gtcgtagcgc tcctcggcgt agaacagccc ctggaaaagc ccctctacgg catcctcggt
     3721 gggcggctcg cccggacgca tcatgcggta gatcgcaacc ttggccgccc actggtcggc
     3781 ggtctcgtcg atgcgcagcg tctgcgagat gtagccaccg cgatcgagat cgttgatgta
     3841 cagcgtgctc aggccggtga cgccggccag gcgcagcttg tcgagcaggt cctcggtgat
     3901 ctcgtcgttg gcgcgcgcca gcagctcgcc ggtggccgga tcgacgacgt tggtcgcgat
     3961 cacccggccg agcaggaagt cgtccggcac cgccatggtc ttcacgccgg cctggtcgat
     4021 ctcgcggatg tgcttggagg tgatgcgctt ctcgcgcgcg acga
//
LOCUS       DAOQBS010000199         3971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_24742_length_3971_cov_20.098570, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000199 DAOQBS010000000
VERSION     DAOQBS010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24742_length_3971_cov_20.098570"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PKN25_14630"
     CDS             <1..406
                     /locus_tag="PKN25_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HNV92185.1"
                     /translation="GALEIARRARGTPRIANRLLRRVRDYAEVKAGGRITAAVADAAL
                     RMLDVDNLGLDLMDRKLLSAMLEKFGGGPVGLDNIAAAIGESTDTIEDVIEPYLIQQG
                     YLQRTPRGRMATQSIWQHFGLVPPRIGGDLFQ"
     gene            422..775
                     /locus_tag="PKN25_14635"
     CDS             422..775
                     /locus_tag="PKN25_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92186.1"
                     /translation="MRSLQVVMLMLCVLGGMFLLVRAPEFFMPAQWDPSIGRSFDPLA
                     SRLLGAGLLALATAGAGYLHAMYYSMPRRLPGPAAQRRHFALLLAALVLIGAALARAE
                     VGPNPEYRAPLRTGS"
     gene            complement(875..1387)
                     /locus_tag="PKN25_14640"
     CDS             complement(875..1387)
                     /locus_tag="PKN25_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92187.1"
                     /translation="MSPTAHPEHPNAASARRTIKAVALVLARHEIRSLRRWFEDFDRD
                     ILLPILLGEIALHNIGALQNGVARRESTRPITATPSVPRDTRPADCGLKPCNAYSIAA
                     ATGLPRETVRRKIGRLIELGWICREDNGHLFITEIALEQFGSMLSSRALGELLETADH
                     VRTLLDDEHD"
     gene            complement(1618..2532)
                     /gene="xerC"
                     /locus_tag="PKN25_14645"
     CDS             complement(1618..2532)
                     /gene="xerC"
                     /locus_tag="PKN25_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNV92188.1"
                     /translation="MGPRRSAEAPAALPERMQAWLAWLATQRRVSPLTLTAYRAGLQA
                     LLRLAEEHPPESLQPADIRRFVARLHGEGQQGRSIARHLSAWRGFYRWLVREHGVRIN
                     PALGIRPPKSPSTLPRVLSPEQANALLDPEPQNLLEIRDIAMFELFYSSGLRVAELAG
                     LDIDSGLDLAEGLVTVTGKRQRQRTVPVGGKAIAALRPWLEIRAGLAAAGERALFVTR
                     DGHRLSTRGIASRLGTRAQALGLGVHVHPHMLRHSFASHLLQSSGDLRAVQELLGHAS
                     IRSTQIYTHLDFQHLAAVYDAAHPRARK"
     gene            complement(2537..3193)
                     /locus_tag="PKN25_14650"
     CDS             complement(2537..3193)
                     /locus_tag="PKN25_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HNV92189.1"
                     /translation="MNAEDVARYLREHPDFLNQHHELFSELTVPHPQHGGQAISLAER
                     QLHALRDKIRALELKLAELIRFGEENDDISTKVHRLSVALLQAGSAAAVRQGLADSLR
                     DDFSVPHVSLKLWGEVGGGVVHTEAFGVGEAARRQVENLRHPWCGVPESIEMTSWFGE
                     AAPHIRSLALMPLRVGGNCIGLLALGSAEPERFYAGMGTLYLGRIADLAAAALAARAG
                     "
     gene            complement(3346..>3971)
                     /gene="dapF"
                     /locus_tag="PKN25_14655"
     CDS             complement(3346..>3971)
                     /gene="dapF"
                     /locus_tag="PKN25_14655"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNV92190.1"
                     /translation="EVEQCGNGARCFVRFVHDKGLTDKAEIRVETRSGIIAPRLRADG
                     LVTVDMGVPELAPAKVPFVSDSDAVVQPLQVGGDTVAITAVSMGNPHAVQVVADVDAA
                     PVVEQGARIERHERFPARVNAGFLQVVDAHEARLRVFERGAGETLACGTGACAAVVAG
                     ILRGLLETPVRVHTRGGELEIAWDGPGTPVRMTGPAVTVFEGEMELA"
BASE COUNT          579 a         1394 c         1409 g          589 t
ORIGIN      
        1 cggcgcgctc gagatcgccc gccgcgcgcg cggcacgccg cgcatcgcca accgcctgct
       61 gcgccgggtg cgcgactacg ccgaggtgaa ggcgggtggc cgcatcaccg ccgcggtcgc
      121 ggacgcggca ctgcgcatgc tggacgtcga caacctgggc ctcgacctga tggaccgcaa
      181 gctgctgtcg gcgatgctgg agaaattcgg cggcggcccg gtcggcctgg acaacatcgc
      241 cgcggcgatc ggcgaatcga ccgacaccat cgaggacgtc atcgagccct acctgatcca
      301 gcagggctac ctgcagcgca ccccgcgcgg gcgcatggcg acgcagtcga tctggcagca
      361 cttcggcctc gtgccgccgc gcatcggcgg cgacctcttc cagtaggcga cggggccgac
      421 cgtgcgctcg ctgcaggtgg tgatgctgat gctgtgcgtg ctgggcggga tgttcctgct
      481 tgtgcgagcg cccgagttct tcatgcccgc acagtgggac ccctcgatcg ggcgcagctt
      541 cgatccgctc gcctcgcgcc tgctcggcgc cggactgctg gcgctcgcca ccgcgggagc
      601 gggctatctt cacgcgatgt actactcgat gccgcgccgc ctgcccggcc cggcagcgca
      661 gcgccgccac ttcgccctgc tgctcgccgc cctggtgctg atcggcgcgg cgctggcgcg
      721 cgccgaggtg gggccgaatc cggaatatcg agcgcccttg cgcacgggtt cgtagggagc
      781 actgcggccg caaagccaca cgttgcccgg cgcgaggctg ctgcggctcg cgtccctcct
      841 gcagaagcct gcacgcccct gcgcgcggcc tgcttcagtc gtgctcgtcg tcgagcaggg
      901 tgcggacgtg gtcggccgtt tcgaggagct cgccgagcgc gcgcgagctg agcatggagc
      961 cgaactgctc gagcgcgatc tcggtgatga agaggtgtcc gttgtcttcg cgacagatcc
     1021 agcccagctc gatcaggcgc ccgatcttgc gccgcaccgt ctcgcgcggc agaccggtgg
     1081 cggcggcgat cgagtaggcg ttgcaaggct tgaggccgca atccgccggg cgcgtgtcgc
     1141 gcggcaccga gggcgtcgcg gtgatcggac gggtgctctc gcggcgcgcg acgccgttct
     1201 gcaaggcgcc gatattatgc agtgcgatct cgccgagcag gatggggagc aggatgtcgc
     1261 ggtcgaagtc ttcgaaccag cgccgcaagc tgcggatctc gtggcgcgcg agcacgagcg
     1321 cgaccgcctt gatcgtgcgg cgtgccgacg ccgcattcgg gtgttccggg tgcgctgttg
     1381 ggctcattgg gctgtcatgg cacgcttcac ccttttttgt tgattccggg agctagcggc
     1441 gttttttaga aaattcttaa attaacagac gccatgagca taacccaagc cgaacgggct
     1501 caatactcgg gaatacccat aatggggaga gtcatattgt gaccccaacc gccctcttct
     1561 gccgctgcga ttccccggat cggggcgtgc gcgccggggt ggcgagcgcg tgacgggcta
     1621 cttgcgcgcg cgcggatgtg ccgcgtcgta caccgcggcg agatgctgga aatcgagatg
     1681 cgtgtagatc tgcgtgctgc ggatgctggc gtggccgagc agttcctgga cagcgcgaag
     1741 atcgccgctc gattgcagaa gatgactcgc aaaactgtgc cgcagcatgt gcggatgcac
     1801 atgcacgccg agcccgagcg cctgagcgcg cgtgcccagc cggctggcga tgccgcgggt
     1861 gctcaggcgg tggccgtcgc gggtcacgaa cagcgcgcgc tcgcccgccg cagcgaggcc
     1921 ggcgcggatc tccagccagg ggcgcagggc cgcgatcgcc ttgccgccca ccggcacggt
     1981 gcgctggcgc tggcgcttgc cggtgaccgt gaccaggccc tcggcgaggt ccagcccgga
     2041 atcgatgtcg agtccggcga gttccgcaac tcgcagaccg gaagaataaa aaagctcgaa
     2101 catcgcaata tcccgaattt ctagcaaatt ctgcggctct ggatcgagca gcgcgttcgc
     2161 ctgctcgggc gagagcacgc gcggcagcgt cgagggggac ttgggcggac ggatgccgag
     2221 cgccggattg atgcgcacgc cgtgctcgcg caccaaccag cggtagaagc ctcgccacgc
     2281 cgacagatgg cgggcgatcg agcgcccctg ctggccctcg ccatgcaggc gggcgacgaa
     2341 gcggcggatg tcggcgggct gcaaggattc gggcgggtgc tcctcggcga ggcgcagcaa
     2401 ggcctgcagg ccggcgcggt aggcggtcag cgtcagcggc gagacccggc gctgggtggc
     2461 gagccaggcc agccaggcct gcatgcgctc gggcagcgcg gccggcgcct cggcggaccg
     2521 ccgcgggccc atggattcag cccgcgcgcg ccgccagcgc cgcggcggcg aggtcggcga
     2581 tgcggcccag gtagagcgtg cccatgccgg cgtagaagcg ctcgggctcg gcgctgccga
     2641 gcgcgagcag gccgatgcag ttgccgccga cgcgcagcgg catcaacgcc agcgaacgga
     2701 tgtgcggcgc ggcctcgccg aaccagctcg tcatctcgat gctctcgggc accccgcacc
     2761 acgggtggcg caggttctcg acctggcggc gcgccgcttc gccgacgccg aaggcctcgg
     2821 tgtgcaccac cccgcccccg acctctcccc acagcttgag gctgacgtgc ggcacggaga
     2881 agtcgtcgcg caggctgtcg gccaggccct ggcgcaccgc ggcggccgag cccgcctgca
     2941 gcagcgcgac cgacaggcgg tgcaccttgg tgctgatgtc gtcgttctcc tccccgaagc
     3001 ggatcagctc ggcaagcttg agttcgagcg cacggatctt gtcgcgcagg gcatggagct
     3061 ggcgctcggc gagcgagatc gcctggccgc catgctgcgg atgcggcacg gtgagctcgc
     3121 tgaagagctc gtgatgctgg ttgaggaaat cggggtgttc gcgcaggtag cgtgcgacat
     3181 cttctgcatt catcgatgct gctccaggcg aggcggctgc cgccccgcgt cgatcgttcg
     3241 tggatggccg ccgcatccgg cgcggtcggc cgcgagtcgg ccggcacgat gccgcacagg
     3301 cgcatcgcgt ccggcccggc aaaatccgga ctcgggcggg ctccctcagg cgagctccat
     3361 ctcgccctcg aaaaccgtga ccgccggtcc ggtcatgcgc accggcgtgc ccgggccgtc
     3421 ccaggcgatc tccagctcgc cgccgcgggt atgcacccgc accggcgtct ccagcaggcc
     3481 gcgcaggatg cccgccacca ccgccgcgca ggcgccggtg ccgcaggcca gggtctcgcc
     3541 ggcgccacgc tcgaacacgc gcagacgcgc ctcgtgcgca tcgaccacct gcaggaagcc
     3601 ggcattgacc cgcgccggga agcgctcgtg gcgctcgatc cgcgcgccct gctccaccac
     3661 cggcgcggca tcgacgtcgg ccaccacctg caccgcgtgc gggttgccca tcgacaccgc
     3721 ggtgatcgcc accgtgtcgc cgcccacctg caaaggctgc acgaccgcgt ccgagtcgga
     3781 cacgaacggc accttcgccg gcgccagctc cggcacgccc atgtccaccg tcaccaggcc
     3841 gtccgcgcgc aggcgcggcg cgatgatgcc ggagcgggtc tcgacacgga tttcggcctt
     3901 gtcggtgagc cccttgtcat gcacgaagcg cacgaagcag cgcgcgccgt tgccgcactg
     3961 ctcgacctcg c
//
LOCUS       DAOQBS010000200         3951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_24918_length_3951_cov_15.776181, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000200 DAOQBS010000000
VERSION     DAOQBS010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24918_length_3951_cov_15.776181"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /gene="scpA"
                     /locus_tag="PKN25_14660"
     CDS             <1..1522
                     /gene="scpA"
                     /locus_tag="PKN25_14660"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNV92191.1"
                     /translation="KIIADIFGYTAQHMPKFNSISISGYHIQEAGANQAIELAFTLAD
                     GVEYVRTGINSGMDVDTFAGRLSFFWAVGMNFYLEIAKMRAARLLWWRLMKQFNPKSA
                     KSMMLRTHSQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEF
                     SARIARNTQIIIQEETHICNVVDPWAGSYMMEKLTQDMADKAWALIEEIESMGGMTRA
                     VESGWAKMKVEECAADKQARIDSGKDVIVGVNKYKLAKEDPIDILDIDNHAVREAQIA
                     RLDKIRATRDTAAVQAALAALTKCAETGEGNLLDLSVKAIRLRATVGEISDALEKVFG
                     RYRANPQAVSGVYGAVVEEQEDWKTLKADIEAFIAEEGRRPRVMIAKLGQDGHDRGAK
                     VVASAFADLGFDIDIGPLFQTPEEAARHAVENDVHAVGCSSLAAGHKTLVPAVIDELK
                     RLGADDIITFVGGVIPAQDYDTLYAAGAKGIFGPGTPIPAAAREVLKQIRAARKAG"
     gene            1730..2773
                     /gene="meaB"
                     /locus_tag="PKN25_14665"
     CDS             1730..2773
                     /gene="meaB"
                     /locus_tag="PKN25_14665"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNV92192.1"
                     /translation="MPAPISTPLDPADQSLVAGVLARQLRPLAKAITLIESQREDHQA
                     RAQKVLEALLPHTGGAMRVGISGVPGVGKSTFIEALGLYLIEQGLRVAVLAVDPSSSV
                     TGGSILGDKTRMELLSQNPAAFIRPSPSACSLGGVAEKTRETMLVCEASGFDVIIVET
                     VGVGQSETAVAGMTDIFCLLQLPNAGDDLQAIKKGIMEIADLIVINKADIDAGAAMRA
                     KAQIKTALHMLRPASPNWTVPVLTLSALQKQGIADFWKAVCDYREALGASGEFAAKRR
                     HQALAWMWEMIDSGLRSRFRSHPRVREELPALARAVEEGDTTPAAAAFRLLGHLQSNE
                     QGRLPRQPAETEI"
     gene            2789..>3951
                     /locus_tag="PKN25_14670"
     CDS             2789..>3951
                     /locus_tag="PKN25_14670"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNV92193.1"
                     /translation="MQDIIRQLEKKRELARLGGGQKRIDAQHRKGKLTARERIELLLD
                     DDSFEEWDMYKEHRCTEFGMDAEHIPGDGVVTGYGTVNGRLVFVFSQDFTVFGGSLSE
                     THAEKICKVMDHAMKVGAPVIGLNDSGGARIQEGVDSLGGYADVFQRNVLASGVIPQI
                     SLIMGPCAGGAVYSPAMTDFIFMVKDSSYMFVTGPEVVKTVTHEEVTAEELGGAVTHN
                     TKSGVADLAFENDVEALMMTRRLIGFLPSSNREKPPAIPCADSADRIDLSLDTLVPEN
                     PNQPYDMKELIFKMVDDGDFFELQPDYAKNIIIGLARMEGSPVGIVANQPLVLAGCLD
                     IKSSIKAARFVRFCDAFNIPVVTLVDVPGFMPGTAQEYGGIIKHGAKLLYAYA"
BASE COUNT          729 a         1411 c         1231 g          580 t
ORIGIN      
        1 gaagatcatc gccgacatct tcggctacac cgcgcagcac atgccgaagt tcaacagcat
       61 ctcgatttcc ggttaccaca tccaggaggc gggcgccaac caggccatcg agctcgcctt
      121 cacgctcgcc gacggtgtcg agtacgtgcg caccggcatc aactccggca tggacgtcga
      181 caccttcgcc ggccgcctgt ccttcttctg ggcggtgggc atgaacttct acctcgagat
      241 cgccaagatg cgcgccgcgc gcctgctgtg gtggcggctc atgaagcagt tcaaccccaa
      301 gagcgccaag tcgatgatgc tgcgcacgca ctcgcagacc tccggctggt cgctcaccga
      361 gcaggacccc tacaacaacg tcgtgcgcac cacgatcgag gccatggctg cggtgttcgg
      421 cggcacccag tcgctgcaca ccaacgcgct cgacgaggcg atcgcgctgc ctaccgagtt
      481 ctccgcccgc atcgcgcgca acacccagat catcatccag gaagagaccc acatctgtaa
      541 cgtggtcgac ccctgggccg gctcctacat gatggagaag ctcacccagg acatggccga
      601 caaggcctgg gcgctgatcg aggagatcga gtccatgggc ggcatgaccc gcgcggtcga
      661 atccggctgg gccaagatga aggtcgagga gtgcgccgcc gacaagcagg cgcgcatcga
      721 ctcgggcaag gacgtgatcg tcggcgtgaa caagtacaag ctcgccaagg aagacccgat
      781 cgacatcctc gacatcgaca accatgcggt gcgcgaggcg cagatcgccc gcctcgacaa
      841 gatccgcgcc acccgcgaca ccgccgcggt gcaggccgcg ctcgctgcgc tcaccaagtg
      901 cgccgagacg ggcgaaggca acctgctcga cctgtcggtc aaggccattc gcctgcgtgc
      961 caccgtcggc gagatctccg acgcgctcga gaaggtcttc ggccgttacc gtgccaatcc
     1021 gcaagccgtt tccggcgtct atggagccgt cgtggaagaa caggaagact ggaagaccct
     1081 caaggccgac atcgaggcct tcatcgccga agaaggccgc cgcccgcgcg tgatgatcgc
     1141 caagctcggc caggacggcc acgaccgcgg cgccaaggtg gtggcctcgg ccttcgccga
     1201 cctcggtttc gacatcgaca tcggcccgct cttccagacc ccggaagaag ccgcccgcca
     1261 cgcggtcgag aacgacgtcc atgcggtcgg ctgctccagc ctcgccgccg gccacaagac
     1321 cctggtcccg gccgtcatcg acgagttgaa gcgcctcggc gccgacgaca tcatcacttt
     1381 cgtcggtggc gtgatcccgg cgcaggacta cgacacgctg tacgccgccg gcgccaaggg
     1441 catcttcggc cccggcacgc cgatcccggc ggccgcacgc gaggtgctca agcagatccg
     1501 cgccgcacgc aaggcggggt gaggtccccg caggagcgac gcaagtcgcg atcgcagcgc
     1561 ccgggcaggc gccgccgacc tcacgcttcc cgtaggagcg acgcaagtcg cgattgcagc
     1621 ggatcagcgg gcggcgtgcg catcgtcgag gcgggaatcg cgacttgcgt cgctcctaca
     1681 agaaccacga accgcaaacc gtagaacgga cgcccctata aaacccgaca tgcctgctcc
     1741 gatatccacc cctctcgatc ccgccgacca gtcgctggtc gctggcgtgc tggcgcgcca
     1801 gctgcggccg ctggccaagg ccatcacgct gatcgaatcg cagcgcgagg accatcaggc
     1861 gcgcgcgcag aaggtgctcg aggcgctgct gccgcatacc ggcggggcga tgcgggtggg
     1921 gatctcgggt gtgccgggcg tgggcaagag caccttcatc gaggcgctcg ggctctacct
     1981 catcgagcag ggcctgcgcg tcgcggtgct cgcggtcgat ccgtcctcgt cggtcaccgg
     2041 tggctccatc ctcggcgaca agacgcgcat ggagctgctg tcacagaacc ccgcggcctt
     2101 catccgcccc agcccctcgg cctgcagcct cggcggcgtg gccgaaaaga cgcgcgagac
     2161 catgctggtg tgcgaagctt cgggcttcga cgtcatcatc gtcgagaccg tcggcgtcgg
     2221 gcagtccgag accgcggtcg ccggcatgac cgacatcttc tgcctgctgc aactgcccaa
     2281 cgccggtgac gacctgcagg cgatcaagaa agggatcatg gagatcgccg acctgatcgt
     2341 catcaacaag gccgacatcg acgccggtgc ggccatgcgc gccaaggcgc agatcaagac
     2401 cgcgctgcac atgctgcgcc cggccagccc caactggacc gtgccggtgc tcaccctgtc
     2461 ggcgctccag aagcagggca tcgccgactt ctggaaggcg gtgtgcgact accgcgaggc
     2521 gctcggcgcc tccggggagt tcgccgccaa gcgtcgccac caggcattgg cctggatgtg
     2581 ggagatgatc gattccgggc tgcgcagccg tttccgcagc catccccgcg tgcgcgagga
     2641 acttcccgcc ctcgcacgcg cggtcgagga gggcgacacc acgcccgcgg ccgcggcctt
     2701 ccggctgctc ggccacctcc aatccaacga acagggccga ctgccgcgac agccggccga
     2761 aaccgagatc tgacaggaga tcgcactcat gcaagacatc attcgccagc tcgagaaaaa
     2821 gcgcgaactg gcccgcctcg gtggcggcca gaagcgcatc gacgcgcagc atcgcaaggg
     2881 caagctcacc gcgcgcgagc gcatcgaact gctgctcgac gacgacagct tcgaagagtg
     2941 ggacatgtac aaggagcacc gctgcaccga gttcggcatg gatgccgagc acatccccgg
     3001 tgacggcgtg gtcaccggct acggcaccgt caacggccgc ctggtcttcg tgttctcgca
     3061 ggacttcacg gtcttcggcg gctcgctctc cgagacccac gccgagaaga tctgcaaggt
     3121 catggaccac gccatgaagg tcggcgcccc ggtcatcggc ctcaacgact cgggcggagc
     3181 gcgcatccag gaaggcgtgg actcgctcgg cggctacgcc gacgtgttcc agcgcaacgt
     3241 gctggcctcc ggggtcatcc cgcagatctc gctgatcatg ggcccctgcg ccggcggcgc
     3301 ggtgtacagc ccggcgatga cggacttcat cttcatggtc aaggactcgt cctacatgtt
     3361 cgtgaccggt cccgaggtcg tgaagaccgt gacccacgag gaagtcaccg ccgaggagct
     3421 cggcggcgcc gtcacccaca acaccaagtc cggcgtcgcc gacctcgcct tcgagaacga
     3481 cgtcgaggcg ctgatgatga cgcgtcgcct gatcggcttc ctgccctcca gcaaccgcga
     3541 gaagccgccc gcgatcccct gcgccgactc cgccgaccgc atcgacctgt cgctcgacac
     3601 cctggtcccg gagaacccga atcagcccta cgacatgaag gagctgatct tcaagatggt
     3661 cgacgacggc gatttcttcg agctgcagcc cgactacgcc aagaacatca tcatcggcct
     3721 ggcgcgcatg gaaggctcgc cggtgggcat cgtcgccaac cagccgctcg tgctggccgg
     3781 ctgcctcgac atcaagagct cgatcaaggc cgcccgcttc gtgcgcttct gcgacgcctt
     3841 caacatcccg gtcgtcaccc tggtcgacgt ccccggcttc atgcccggca ccgcccagga
     3901 atacggcggc atcatcaagc acggcgccaa gctgctctac gcctacgccg a
//
LOCUS       DAOQBS010000201         3823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_26177_length_3823_cov_12.014331, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000201 DAOQBS010000000
VERSION     DAOQBS010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26177_length_3823_cov_12.014331"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..1052
                     /locus_tag="PKN25_14675"
     CDS             324..1052
                     /locus_tag="PKN25_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNV92194.1"
                     /translation="MSQESPTFSPLYRQIKALILQALEAGEWRPGQAIPSEQELAARF
                     NVSQGTVRKAIDEMAADNLLVRKQGKGTFVASHNDPRALFRFLRLVPVDGDLSPPQSI
                     PLDCWRAKAGQEASRMLAIEPGAPIIIVRRLLKFAARPVVIDEIYLPGEVFQGLSAET
                     LQGWNGSLYSLFETRFGVRMIRAQERIRAVAADRGAAEALGVQEGTPLLSVERVTYTY
                     GDRPVEWRRGLYSTAEHFYLNELN"
     gene            1152..1541
                     /gene="sdhC"
                     /locus_tag="PKN25_14680"
     CDS             1152..1541
                     /gene="sdhC"
                     /locus_tag="PKN25_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNV92195.1"
                     /translation="MSEVTARKPRPKYLALNEIRLPLPGIVSILHRISGVGIFLMLPF
                     LLYLLDRSLGSPETFETFAAVVDNWFIKLVLFGLLWAYLHHFCAGIRFLLLDLHKGLE
                     LEEARKSSKIVLIVSLTLTLIIGVVLW"
     gene            1535..1885
                     /gene="sdhD"
                     /locus_tag="PKN25_14685"
     CDS             1535..1885
                     /gene="sdhD"
                     /locus_tag="PKN25_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNV92196.1"
                     /translation="MVKQRIVVGAHYGLKDWIAQRATAVYMAAYTIIFALCAFSLREL
                     NYQEWSGLFSHGLFKFLSFLFFLSVFYHAWIGIRDLWMDYIKPNGVRLALHLVTIFVL
                     VGYAGWAAQILWRL"
     gene            1888..3681
                     /gene="sdhA"
                     /locus_tag="PKN25_14690"
     CDS             1888..3681
                     /gene="sdhA"
                     /locus_tag="PKN25_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HNV92197.1"
                     /translation="MNVAKRTFDAVIVGAGGAGLRAALQLSEAGLKTAVLTKVFPTRS
                     HTVAAQGGVAASLGNSTEDNWHWHMYDTVKGSDWLGDQDAIEFMCKKANEVVIELEHY
                     GMPFDRTDNGKIYQRPFGGHSMNYGQAPVMRSCAAADRTGHAMLHALYQRNVRANTQF
                     FVEWMALDLIRDADGDVLGVTAMEMETGEVSIFHAKATIFATGGAGRIYYSSTNAFIN
                     TGDGVGMAARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILRNAQGERFMERY
                     APNLKDLASRDVVSRAMTTEINEGRGCGPDKDHVLLDITHLAPDTIMKRLPGIREIAI
                     QFAGVDPIKAPIPVVPTCHYQMGGIPTNYKGQVVVPKDGNPNSPVAGFYAAGECACAS
                     VHGANRLGTNSLLDLLVFGKSAGETVVEDFQSGQLKLKPLPADAADVSLARLARLDGQ
                     TNGESVFDVGLEMRRTMQKHAGVFRFDNLLKEGVQKIGEVAERAKRTQIADKSKVWNV
                     ARMEALELDNLIEVARATMVSAEARKESRGAHVRDDAPDTAENPNGRDDANWLKHTLW
                     YSEGNRLDYKPVNLKPLSDDVEPIALAKRTY"
     gene            3702..>3823
                     /gene="sdhB"
                     /locus_tag="PKN25_14695"
     CDS             3702..>3823
                     /gene="sdhB"
                     /locus_tag="PKN25_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765285.1"
                     /note="part of four member succinate dehydrogenase enzyme
                     complex that forms a trimeric complex (trimer of
                     tetramers); SdhA/B are the catalytic subcomplex and can
                     exhibit succinate dehydrogenase activity in the absence of
                     SdhC/D which are the membrane components and form
                     cytochrome b556; SdhC binds ubiquinone; oxidizes succinate
                     to fumarate while reducing ubiquinone to ubiquinol; the
                     catalytic subunits are similar to fumarate reductase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNV92198.1"
                     /translation="MSKRTVHFKIYRYDPDKDEKPYMQDISVELEPSDKKLLDAL"
BASE COUNT          678 a         1285 c         1181 g          679 t
ORIGIN      
        1 ctggcgatgc ggaacagcag gctgtagccg atctggccgg cggcgccggt gacagcgacg
       61 cgcatggggg ctttgctcat gtgattactc cctcgagtga gacggtcgta ttaaggtgct
      121 ggaaacctgc ccggcaccgt cgcggcaccc gcaggcggga tcgtcgcggg agctggcgcg
      181 gcgccccccc gcgtgcgcga ggaccaagct gggcgccggc atactagacc gggcttgaat
      241 gcctgtcaaa tgccatctta tatcttatat aagacacaag accaaagata gacgtttgcg
      301 aaaatttatg ctccaatgcg cgcatgtcgc aggagtcgcc taccttcagt ccgctctatc
      361 gccagatcaa ggccctgatc cttcaggcac ttgaggctgg ggaatggcgt cccggccagg
      421 ccattccgag cgagcaggaa ctcgccgcgc gcttcaacgt gtcgcagggc accgtgcgca
      481 aggctatcga cgagatggca gccgacaacc tgctcgtgcg caagcagggg aagggcacct
      541 tcgtggcctc tcacaacgat ccgcgcgcgc tcttccgttt cctccgcctc gtgccggtgg
      601 atggtgacct ctcgcctccg caaagcatac cgctggattg ctggcgtgcg aaggccgggc
      661 aggaggcttc gcgcatgctc gcgatcgagc ccggcgcccc gatcatcatc gtccgccgct
      721 tgttgaagtt tgccgcccga cccgtcgtca tcgacgagat ctatctgccc ggcgaggtgt
      781 tccagggact cagcgccgag accctgcagg gctggaacgg ttcgctctac agtctcttcg
      841 agacccgctt cggcgtgcgc atgatccgcg cccaggagcg catccgcgcg gtcgccgccg
      901 accgtggcgc cgccgaggcg ctcggcgtcc aggaaggcac gccgctgctg tcggtcgagc
      961 gtgtcacgta cacctatggc gatcggcccg tggagtggcg tcgcgggctg tattcgacgg
     1021 cggaacattt ttatctgaat gagctgaatt gaggggcgcg catgggctgc aggccgggac
     1081 cggcgctata attttccgtc ttttttcgga gtccgcgacg ggggtcgtcg cacatgcaga
     1141 ggggagtctt catgtcagaa gtaacagcgc gcaaaccaag gccgaagtac ctggccctca
     1201 acgagatccg gctgccgttg ccgggcatcg tctcgatcct tcaccgcatc agcggcgtcg
     1261 gcatcttcct gatgttgcct ttcctgttgt acctgctcga tcgcagcctc ggctctccgg
     1321 agaccttcga gaccttcgcc gcggtggtcg acaactggtt catcaagctc gtcctgttcg
     1381 gtctgctgtg ggcctacctg catcacttct gtgccggcat ccgcttcctg ctgctcgacc
     1441 tgcacaaggg tctggagctc gaggaagcac gcaagtcctc caaaatcgtt ctgatcgtga
     1501 gccttacgct caccttgatc atcggggtgg tcctatggtg aaacagcgta tcgtcgtcgg
     1561 cgcgcactac ggtctgaagg actggatcgc gcaacgcgcc accgccgtct acatggcggc
     1621 ctacaccatc atcttcgcgc tctgtgcgtt cagcctgcgc gagctcaact atcaggaatg
     1681 gtccggcctg ttctcgcacg gcttgttcaa gttcctcagc ttcctgttct tcctgtcggt
     1741 cttctatcac gcctggatcg gcatccgcga tctctggatg gactacatca agccgaacgg
     1801 tgtgcgcctc gcgctgcacc tggtcaccat tttcgtgctc gtcggctatg ccggctgggc
     1861 agcccagatc ctgtggaggc tttaagcgtg aacgtcgcga agcgtacctt tgatgcggtc
     1921 atcgtgggcg ccggcggtgc cggcctgcgc gcggccctgc aactctccga agccggcctc
     1981 aagaccgccg tgctgaccaa ggtgttcccg acccgctcgc acaccgttgc ggcgcagggc
     2041 ggcgtcgccg cctcgctggg caactcgacc gaggacaact ggcactggca catgtacgac
     2101 accgtcaagg ggtcggactg gctgggcgac caggatgcca tcgagttcat gtgcaagaag
     2161 gccaacgagg tcgtcatcga gctcgagcac tacggcatgc ccttcgaccg caccgacaac
     2221 ggcaagatct accagcgtcc gttcggcggc cactcgatga actacggcca ggcgccggtg
     2281 atgcgctcct gcgcggcggc cgaccgtacc ggccacgcca tgctgcacgc gctctaccag
     2341 cgcaacgtgc gcgccaacac ccagttcttc gtcgaatgga tggcgctcga cctgatccgc
     2401 gacgccgatg gcgacgtgct cggcgtcacc gcgatggaaa tggagaccgg cgaggtctcg
     2461 atcttccacg ccaaggccac catcttcgcc accggcggtg ccggccgcat ctactattcc
     2521 tcgaccaacg ccttcatcaa caccggcgac ggcgtgggca tggcggcgcg tgccggcatc
     2581 ccgctcgagg acatggagtt ctggcagttc caccccaccg gcgttgccgg cgcgggcgtg
     2641 ctcatcaccg aaggcgtgcg cggcgagggc ggcattctgc gcaacgccca gggcgagcgc
     2701 ttcatggagc gctacgcgcc caacctcaag gatctcgcct cgcgcgacgt ggtttcgcgc
     2761 gcgatgacca ccgagatcaa cgaaggtcgt ggctgcggtc ccgacaagga tcacgtgctg
     2821 ctcgacatca cgcaccttgc gcccgacacc atcatgaagc gcctgcccgg catccgcgag
     2881 atcgcgatcc agttcgccgg cgtcgacccg atcaaggcgc cgatcccggt cgtgccgacc
     2941 tgccactacc agatgggcgg catcccgacc aactacaagg gccaggtcgt ggtgccgaag
     3001 gacggcaacc cgaacagccc ggtcgccggc ttctacgccg cgggcgagtg cgcctgcgcc
     3061 tcggtgcacg gcgccaaccg cctcggcacc aactcgctgc tcgacctgct ggtgttcggc
     3121 aagtcggcgg gcgagaccgt ggtcgaggac ttccagtccg gccagctcaa gctcaaaccg
     3181 ttgccggccg acgctgccga cgtctcgctc gcccgcctgg cccgcctcga cggccagacc
     3241 aacggcgaga gcgtgttcga cgtcggcctc gagatgcgtc gcacgatgca gaagcacgcc
     3301 ggcgtgttcc gcttcgacaa cctgctcaag gaaggcgtgc agaagatcgg cgaggtcgcc
     3361 gagcgtgcca agcgcaccca gatcgcggac aagtccaagg tctggaacgt cgcccgcatg
     3421 gaagcgctcg agctcgacaa cctgatcgag gtcgcgcgcg ccaccatggt ctcggccgag
     3481 gcccgcaagg aatcgcgtgg cgcccacgtg cgcgacgacg cgccggatac ggcggagaac
     3541 cccaacggcc gcgacgacgc caactggctc aagcacaccc tctggtattc ggaaggcaat
     3601 cgcctcgatt acaagccggt gaacctgaag ccgctgtccg acgacgtcga gccgatcgcg
     3661 ctcgccaagc gtacctattg agcgtccggg agaaaaaatt catgagcaag cgtaccgttc
     3721 atttcaagat ctaccgctac gatccggaca aggacgagaa gccctacatg caggacatct
     3781 cggtcgagct cgagccgtcc gacaagaagc tgctcgacgc gct
//
LOCUS       DAOQBS010000202         3757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_26859_length_3757_cov_12.896542, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000202 DAOQBS010000000
VERSION     DAOQBS010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26859_length_3757_cov_12.896542"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..2256)
                     /locus_tag="PKN25_14700"
     CDS             complement(37..2256)
                     /locus_tag="PKN25_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV92199.1"
                     /translation="MKPEHRAVPAAPIAPIAGRMPPRAKKLASLLHLGVPGALAGFAC
                     LAGAQQAAPAAAEAVTLNPVVVTGAPTATESFDLPYSVDSVDMRANQAGNLGVNASEA
                     LAGVPGLVIQNRQNYAQDLQISVRGFGARAPFGVRGVKLIADGIPATNPDGQGQAATF
                     NLDTAERIEVMRGPFSTVYGNHAGGVIQLFSRTGEGAPRVRASALGGSWGTTKFGIGA
                     EGEKDGIGFVLDASRFDTDGYRDHSKTRRDQGFAKLTLNPDEDSTLTLVANGLRQPET
                     QDPLGVTWETYERDPQSLTIDPLDPATPKRSYADRYNTRKSIHHVQGGATYERRLGED
                     RLQVSAYAGSRAVTQYQSFSRGFQSSPRHAGGVVDFDRDFHGMGLRWISQRALGSGEL
                     TLTAGIDYDRSEDDRRGYENFVGSTLGVKGALRRDELDTITSIDPYVQALWKSGPWQW
                     SLGARHSRVSFKVEDRYIKTDNPDDSGSVRFSKTTPALGVSYALSPATNLYASVGAGY
                     ETPTLNELSYATPTTGFNFGLRPSTSRQAEIGIKTFVSDNARINAALFRIETDDEIVV
                     LASEDGRTSYQNAGRTLRQGLELAAQAELTRTLTARASLTWMQAEYDEAFSSRGTTVA
                     AGNRIPGVPRIAAFAELAWEATPGVTLAGEVLHRGEVEVSDLNNDHVAPAYTLVNLRL
                     SAEQRQGPWTFGQLLRVDNVLDREYVGSVIVGDSNKRYYEPGPERSVYAGVSASYRF"
     gene            complement(2404..3237)
                     /locus_tag="PKN25_14705"
     CDS             complement(2404..3237)
                     /locus_tag="PKN25_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV92200.1"
                     /translation="MSVLLHIADTHFGTEQPPVVEALVALAAQQRPDLVVLAGDITQR
                     ARPAQFRAAKAFVDRLGAPVLAVPGNHDIALFDLWARLTRPYARYAEVFGADLEPVHA
                     SADLLVVGVNTTRAWRHKNGEVSAAQVDRVAGLLASASPQQLRVVVVHQPAAVSCPGD
                     RADLVRGHQAALRAWSVAGADLVLGGHIHLPYTLAPPGLARRLWVLQAGTAVSWRTRA
                     EVPNSVNILRWGEAAGAHEHPAQCGAAEGACCLVEQWDFVRKDRRFVRKAVTRVHPER
                     P"
     gene            complement(3248..>3757)
                     /locus_tag="PKN25_14710"
     CDS             complement(3248..>3757)
                     /locus_tag="PKN25_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNV92201.1"
                     /translation="REAYKARFGRSRWVAFVAGCATLLRAQRRLRLHIETGSTVRDVQ
                     TLTLFVGNNRLQLQQFGAEPADTLAGTPGDGCLAALMLRPIGTLSMLGLMLHGAMGRL
                     GEAAGVDSFEFTHMVVKPRLAPGRREVVVAFDGEVARMRAPIEIRVLDKPLYLLQAPD
                     PAGTPEAGP"
BASE COUNT          515 a         1308 c         1373 g          561 t
ORIGIN      
        1 ggcggcggat cgtcaggagt ccgcgccggc cgtcgctcag aaccggtagc tcgcgctcac
       61 cccggcatac acgctgcgct cggggccggg ctcgtaatag cgcttgttgc tgtcgccgac
      121 gatcaccgag ccgacgtatt cgcgatcgag cacgttgtcc acccgcagca actggccgaa
      181 ggtccacggc ccctggcgtt gctcggcgga caggcgcaga ttgacgagcg tgtaggccgg
      241 tgcgacgtgg tcgttgttga ggtcgctgac ctcgacctct ccgcgatgca gcacctcgcc
      301 cgcgagcgtc acgcccggcg tggcttccca ggccagctcg gcgaaggccg cgatgcgcgg
      361 aacacccgga atgcggtttc cggccgccac ggtggtcccg cggctgctga acgcctcgtc
      421 gtactcggcc tgcatccagg tcagcgaggc ccgcgccgtc agcgtgcggg tcagctcggc
      481 ctgggcggcc agctcgagcc cctggcgcag cgtgcgcccg gcgttctggt agctggtccg
      541 gccgtcctcc gaggccagga cgacgatctc gtcgtcggtc tcgatgcgga acagcgcggc
      601 gttgatccgc gcgttgtcgc tcacgaaggt cttgatgccg atctcggcct ggcggctggt
      661 gctcggacgc agcccgaaat tgaagccggt ggtcggggtc gcgtacgaca gctcgttgag
      721 cgtgggcgtc tcgtagcctg cacccacgct ggcgtagagg ttggtcgcgg ggctcagcgc
      781 atagctcacc ccgagcgccg gggtggtctt gctgaagcgc acgctgccgc tgtcgtcggg
      841 gttgtcggtc ttgatgtagc ggtcttccac cttgaacgaa acgcgcgaat ggcgggctcc
      901 gagcgaccac tgccacggtc cgctcttcca cagcgcctgg acataggggt cgatgctggt
      961 gatggtgtcg agctcgtcgc ggcgcagcgc tcccttcacc ccgagcgtgc tgccgacgaa
     1021 gttctcgtag ccgcgccgat cgtcctcgga gcgatcgtag tcgatgccgg cggtcagggt
     1081 gagctcgccg ctccccagcg cgcgttgcga aatccagcgc aggcccatgc cgtggaagtc
     1141 gcggtcgaag tcgaccacgc cgcccgcatg gcgcgggctg gactggaaac ccctggagaa
     1201 ggactggtac tgggtgaccg cgcggctgcc cgcgtaggcc gacacctgca ggcgatcctc
     1261 gccgaggcgg cgctcgtagg tggccccgcc ctgcacgtgg tggatgctct tgcgcgtgtt
     1321 gtagcggtcc gcgtaggagc gcttcggcgt ggcggggtcg agcggatcga tcgtgagcga
     1381 ctgcggatcg cgctcgtagg tctcccaggt cacccccagc ggatcctgcg tctccggctg
     1441 gcgcagcccg ttggccacca gggtcagcgt gctgtcctcg tccgggttca gcgtcagctt
     1501 ggcgaagccc tggtcgcggc gggtcttgct gtggtcgcgg tagccgtcgg tgtcgaagcg
     1561 cgaggcgtcg agcacgaagc cgatgccgtc cttctcgccc tcggcgccga tgccgaactt
     1621 ggtcgtgccc cagctgccgc cgagcgcgct cgcgcgcacg cgcggcgcgc cttcaccggt
     1681 gcgcgagaag agctggatga ccccgccggc gtggttgcca tagacggtgg agaagggccc
     1741 gcgcatgacc tcgatgcgtt cggcggtgtc gaggttgaag gtggcggcct ggccctggcc
     1801 gtccgggttg gtggcgggga tgccgtcggc gatgagcttg acgccgcgca cgccgaaggg
     1861 cgcgcgcgcg ccgaagccgc gcaccgagat ctgcaggtcc tgggcgtagt tctggcgatt
     1921 ctggatcacc agtccgggca cgccggcgag cgcctcggag gcattcacgc cgaggttccc
     1981 ggcctggttg gcgcgcatgt ccaccgaatc gaccgagtag ggcaggtcga agctctcggt
     2041 ggcggtgggt gcgccggtga ccaccaccgg attgagcgtc accgcctccg cggccgccgg
     2101 cgcggcctgc tgcgcgccgg cgaggcaggc gaagccggcc agcgcacccg gaacgccgag
     2161 gtggagcagg gaggcgagct tcttcgcgcg cggcggcatg cggccggcga tgggggcgat
     2221 gggggcggca gggactgcgc ggtgctcggg tttcacggtg agactcctcg gcgggcgtga
     2281 ctttccggag gattgtcacc gatggtcgac gccgcccgcc tcgacgcaag ctccagcgcc
     2341 ccctacagaa aatcatgagt cctgcgcccg tggccccacg cggcgcccgc aggggcctcg
     2401 cgatcagggg cgttccgggt gcacgcgcgt gaccgccttg cggacgaagc gtcgatcctt
     2461 gcgcacgaag tcccactgct cgacgaggca gcatgcaccc tccgcagcgc cgcactgcgc
     2521 cgggtgctcg tgcgccccgg cggcctcgcc ccagcgcagg atgttcaccg aattgggcac
     2581 ctccgcacgc gtgcgccacg agaccgcggt gccggcctgg agcacccata gccgccgtgc
     2641 aagaccgggc ggtgccagcg tgtagggcag atggatgtgt ccgccgagga cgagatcggc
     2701 cccggcgacc gaccacgccc gcagggcggc ctggtggccg cgcaccaggt cggcccggtc
     2761 ccccggacag gacaccgccg cgggctgatg caccaccacc acgcgcaact gctgcggact
     2821 cgccgaggcc agcaggcctg ccacacggtc gacctgcgcg gccgacactt ccccgttctt
     2881 gtgccgccag gcgcgcgtgg tgttgacccc gacgaccagc aggtccgccg acgcgtgaac
     2941 cggctcgagg tcggcgccga acacctcggc gtagcgcgcg taggggcggg tcaggcgcgc
     3001 ccagagatcg aacaaggcga tgtcgtggtt gccgggcacc gccaggaccg gcgcccccag
     3061 tcggtcgacg aaggccttcg ccgcgcggaa ctgggcgggt cgcgcgcgct gcgtgatgtc
     3121 gcccgcgagc accaccaggt ccggccgctg ctgcgctgcg agggcgacca gggcctcgac
     3181 caccggcggc tgctcggtgc cgaagtgggt gtcggcgatg tgcagcagga cgctcatgcc
     3241 gcagccctca tggcccggcc tcgggcgtgc ctgccgggtc aggggcctgg agcaggtaca
     3301 agggcttgtc gagcacgcgg atctcgatgg gcgcgcgcat ccgcgccacc tcgccgtcga
     3361 aggccaccac gacctccctg cgaccgggcg ccagcctggg cttcaccacc atgtgggtga
     3421 actcgaagct gtccaccccg gcggcttcac ccagccggcc catcgcccca tgaagcatca
     3481 gcccgagcat cgacagggtg ccgatgggcc gcagcatcag cgcggccagg cagccatcgc
     3541 cgggcgtgcc ggcgagggtg tccgcgggct ccgcgccgaa ctgctgcagc tgcagccggt
     3601 tgttgccgac gaagagggtc agcgtctgca cgtcgcgaac ggtggagccg gtctcgatgt
     3661 gcaggcgcag ccggcgctgc gcacgcagca gcgtcgcgca gcccgcgacg aacgccaccc
     3721 agcggctacg gccgaagcgg gccttgtagg cctcgcg
//
LOCUS       DAOQBS010000203         3636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_28207_length_3636_cov_16.186540, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000203 DAOQBS010000000
VERSION     DAOQBS010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28207_length_3636_cov_16.186540"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PKN25_14715"
     CDS             complement(<1..583)
                     /locus_tag="PKN25_14715"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927113.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNV92202.1"
                     /translation="MKYLSTRGHAGQPANPEFCDILLGGLAPDGGLYLPETYPQVTRA
                     ELDAWRGLSYAELAFAILSKFITDIPAVDLKAICAKTYTAEVYRHVRAGGDARDITPV
                     HWLEKDADGRGRLGLLELSNGPTLAFKDMAMQLLGNLFEYVLAKRGETINILGATSGD
                     TGSAAEYAMRGKHGVRVFMLSPHGKMSAFQRAQM"
     gene            824..1675
                     /locus_tag="PKN25_14720"
     CDS             824..1675
                     /locus_tag="PKN25_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HNV92203.1"
                     /translation="MNLGNILGQILQQGMGQQGRSRLDHAMGAGGIGDVLGGILGGQA
                     AGGTAGSAGGGLGDLLGGVLGGGRGGSAAGGMGGLGEILGGALGGGRSTSGGGLGDLL
                     GGLLGGSAGGGTGTRSGGGNAGMAILATIAMAALKNWTDGRRAQAAMAPDTAGFAAPE
                     LETMTEPATAELIVRAMISAAKADGEVSEDEIQRIVGRIGADGLSEEEKQFMIAELRR
                     PLDLAALVAEVPNEMVAAEVYAASLLAIELDTPAEAAYLRQLAQALRLDGATLSRLHE
                     ITGATAI"
     gene            complement(1733..1945)
                     /locus_tag="PKN25_14725"
     CDS             complement(1733..1945)
                     /locus_tag="PKN25_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNV92204.1"
                     /translation="MSDRVYKLVEIVGTSHESTDAAIRNAIETAARTIRHIDWFEVVE
                     TRGHVVDGKVAHFQVTLKLGFRLESE"
     gene            complement(1987..2133)
                     /locus_tag="PKN25_14730"
     CDS             complement(1987..2133)
                     /locus_tag="PKN25_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92205.1"
                     /translation="MAFKLAVFTAIVVAIAVLGVRGYVASVRGFEAARALRGEDRSAD
                     PDAD"
     gene            complement(2286..2948)
                     /locus_tag="PKN25_14735"
     CDS             complement(2286..2948)
                     /locus_tag="PKN25_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNV92206.1"
                     /translation="MKSKVDEVFVGKVRLLSIEGERSGIVKSPAAGPVMLTPTGLEGD
                     EQADLRHHGGLEKALHHYPVEHYAVLAAEWPQCAAALGAGFLGENISTHGLDEHVLCI
                     GDVLRIGAARVQVSQTRSPCWKIDLRLQVEGASRFVEAAGITGWYYRVLQPGRIAAGD
                     TIELLERPNPWLTLADYWDTVSAHRPDAGLLARIAGAPGLAGDKATRWRERAAWLRAQ
                     GG"
     gene            complement(3065..>3636)
                     /locus_tag="PKN25_14740"
     CDS             complement(3065..>3636)
                     /locus_tag="PKN25_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNV92207.1"
                     /translation="KLLGIARRRENGVELRVHPTLIPAKRLIANVEGAMNAVLVQGDA
                     VGATLYYGKGAGAEPTASAVIADLVDVTRLHTSDPEHRVPHLAFQPDQVHDVPVLPIE
                     EVVTSYYLRMRVEDKPGVLADITRILADSGISIEALIQKQAAEGEAHTDIIMLTHQTA
                     EKNANAAIVRIEALPVVQGKVVKLRMEAL"
BASE COUNT          540 a         1286 c         1284 g          526 t
ORIGIN      
        1 acatctgcgc gcgctggaag gcgctcatct tgccgtgcgg cgacagcatg aacacacgca
       61 cgccgtgctt gccgcgcatc gcgtactcgg ccgccgagcc ggtatcgccc gaggtggcac
      121 cgaggatgtt gatggtctcg ccacgcttcg cgagcacgta ttcgaagagg ttgccgagca
      181 gctgcatggc catgtccttg aaggccagcg tcgggccatt ggacagctcg agcaggccga
      241 ggcggccgcg gccgtcggca tccttctcca gccagtgcac cggagtgatg tcgcgcgcgt
      301 cgccgcccgc gcgcacgtgg cgatagacct cggcggtgta ggtcttggcg cagatcgcct
      361 tcaggtcgac ggcggggatg tcggtgatga acttcgacag gatcgcgaac gccagctctg
      421 cgtaggacag gccgcgccag gcgtcgagct cggcgcgcgt cacctgcgga taggtctcgg
      481 gcaggtagag gccgccgtcg ggggcgaggc cgccgagcag gatgtcgcag aactccgggt
      541 tggcgggctg gccggcgtgg ccgcgggtgg agagatactt cacgtgggca gtccttcagg
      601 gtgacgtggt cggcgcgcgc cgtccggcgc aaaggctcga ttctaacgaa ttcggcggac
      661 gaattcggct tgccggcaca ccccaacccc ggcgaggagc ccggacgccg aacgcgggaa
      721 cgacgtcgcg cgccgcagct ccgcacgcgc ccgcgatggg caggacgccc gctgcccgca
      781 caccgataca atgggacctt cacatcatga acgggagtcc gccatgaacc tcggaaacat
      841 cctcggccag atccttcagc aaggcatggg ccagcagggc cgttcgcgcc tcgaccacgc
      901 catgggcgcg ggcggtatcg gagacgtgct cggaggcatt ctcggcggac aggcggcggg
      961 cggcacagct ggcagcgcgg gcggcggcct gggcgatctg ctgggtggcg tgctgggcgg
     1021 cggccgcggc ggctccgcgg cgggcgggat gggaggcctg ggcgagatcc tcggcggcgc
     1081 gctcggtggc ggccgcagca cgagcggagg cggcttgggc gatctgctcg gcggcctgct
     1141 cggaggcagc gccgggggcg gcacgggcac gcgcagcggc ggcggcaacg ccggcatggc
     1201 catcctcgcc accatcgcga tggcggcgct gaagaactgg accgacggcc gccgcgcgca
     1261 ggccgcgatg gcacccgaca cggcgggctt cgccgccccc gaactcgaaa ccatgaccga
     1321 gcccgcgacc gcggagctga tcgtgcgcgc gatgatctcg gcggccaagg ccgacggcga
     1381 agtgagcgag gacgagatcc agcgcatcgt cggccgcatc ggcgccgacg gcctgagcga
     1441 ggaggagaag cagttcatga tcgccgaact gcgtcgcccg ctcgacctcg ccgcgctcgt
     1501 cgccgaggtg cccaacgaga tggtggcggc cgaggtctat gccgcctcgc tgctcgcgat
     1561 cgaactcgac accccggccg aggcggccta cctgcgccag ctcgcccagg cactgcgcct
     1621 ggacggtgcg acgctgtccc gcctgcacga gatcaccggc gccaccgcga tctgagcgcg
     1681 ggtatcgggc cgcaccgccg cacgccaagc tggcgcggcg gcgcgactcg cctcactcgc
     1741 tctcgagccg gaagcccagc ttcagcgtga cctggaagtg cgcgaccttg ccgtcgacca
     1801 cgtggccgcg cgtctccacc acctcgaacc agtcgatgtg gcggattgtg cgcgcagcgg
     1861 tctcgatcgc attgcggatg gcggcatcgg tgctctcgtg cgaggtgccg acgatttcga
     1921 cgagcttgta gacgcgatcg gacatcttgt cctcctcttc ggtgccggcc agcgccggat
     1981 ggctgttcag tccgcgtccg gatccgcgga ccgatcctcc ccgcgcaatg cccgcgcagc
     2041 ctcgaagccg cgcaccgacg ccacatagcc acggacgccg agcacggcga tcgcaaccac
     2101 aatggcggtg aagacggcga gcttgaaggc catgcgaagg cgtcctgcag gcaagggttg
     2161 ggaatcgcgg cacgcgcctc gcgcgcgccg ccgtgcttca gtctaggcgc aaacgcctgt
     2221 ggtgtcgcgc gtgcccggag ctcccgtcac agcccacgcg cgcgcccgcc tcgccggcca
     2281 cttcctcacc ccccctgcgc gcgcagccac gccgcacgct cgcgccagcg cgtcgccttg
     2341 tcgccggcga gcccgggcgc gccggcgatg cgtgcgagca gcccggcatc gggccggtgc
     2401 gcgctcaccg tgtcccagta gtcggcaagc gtgagccagg gattgggacg ctcgagcagc
     2461 tcgatcgtgt cgcccgcggc gatcctgccc ggctgcagca cgcggtagta ccagccggtg
     2521 atccccgccg cctcgacgaa acgcgaggcg ccctcgacct gcagacgcag gtcgatcttc
     2581 cagcacggcg agcgcgtctg gctcacctgc acgcgcgccg cgccgatgcg cagcacgtcg
     2641 ccgatgcaca gcacgtgctc gtccagcccg tgcgtgctga tgttctcgcc gaggaaaccc
     2701 gcacccagcg cggctgcgca ctgtggccac tcggcggcca gcaccgcgta gtgctcgacc
     2761 ggatagtggt gcagcgcctt ctcgaggccg ccgtggtggc gcaggtcggc ctgctcgtcg
     2821 ccctccagcc cggtcggcgt cagcatgacc gggcccgcgg ccggcgactt gacgatgccg
     2881 ctgcgctcgc cctcgatcga cagcaggcgc accttgccga cgaagacctc atccaccttg
     2941 ctcttcatgt cccccaatcc tccctgcctt ccaggtcgca gcaatggcga cccgggatga
     3001 cgtgaacgca agaggccgca cccgggggca cggcctcgtc ttcgatcacg actcgaaact
     3061 gcgctcagag cgcttccata cgcagcttca cgaccttgcc ctgcactacg ggcagcgcct
     3121 cgatgcgcac gatggcggca ttggcgttct tttccgcggt ctggtgggtc agcatgatga
     3181 tgtcggtgtg cgcctcgccc tcggccgcct gcttctggat cagcgcctcg atcgagatcc
     3241 cgctgtcggc caggatgcgg gtgatgtcgg cgagcacgcc cggcttgtcc tcgacccgca
     3301 tgcgcaggta gtaggaggtc acgacctcct cgatcggcag caccggaacg tcgtgcacct
     3361 ggtcgggctg gaaggccagg tggggcacgc ggtgctcggg gtcggaggtg tgcaggcggg
     3421 tgacgtcgac caggtcggcg atcaccgccg aagcggtggg ctcggcgccc gcgcccttgc
     3481 cgtagtagag cgtcgcgccg acggcgtcgc cctgcaccag caccgcattc atcgcaccct
     3541 cgacgttggc gatgaggcgc ttcgccggaa tcagcgtggg gtgcacgcgc agctcgacgc
     3601 cgttctcgcg gcggcgggcg atgccgagca gcttga
//
LOCUS       DAOQBS010000204         3598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_28713_length_3598_cov_14.066046, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000204 DAOQBS010000000
VERSION     DAOQBS010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28713_length_3598_cov_14.066046"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PKN25_14745"
     CDS             <1..902
                     /locus_tag="PKN25_14745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV92208.1"
                     /translation="RRLFEAERFDIVHARSRLPAWVAWLAWRGMPADARPHFVTTVHG
                     MHSVSRYSAIMCAGERVIAVSDTVRDYLRSHYPPTRWPHLADERITVIPRGIDPAEFP
                     RDYPPSDEWLARFHAEFPQIGQRKVLTLPGRLTRLKGHHDFITLVARLVAEGIDVIGL
                     VVGGEDPKRPGYAKEIRERVQAEGLGDRIVFTGHRSDVREIYSVSDCVLSLSSTPESF
                     GRTVLEPLAMGRPVVGYAHGGVAEILAELFPHGAVGKGDVAAATVRAADVIAGRTPVV
                     EHNSRFLLANMQAQTLKLYRSLA"
     gene            899..1723
                     /locus_tag="PKN25_14750"
     CDS             899..1723
                     /locus_tag="PKN25_14750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV92209.1"
                     /translation="MTEWLALSAALAAGWFSNRYAWWRKPVDLRHPRVLMYHMVSPHR
                     PGAKFNGLRVPPERFEAQLDWLKRKGWTFFTVGELWAQWDELPEKSVAITFDDGYADN
                     LHNALPLLDKYDAKATIYIVVDRHDRDWSTAKKAHHNTGELAREPKLSDDELQRLVAS
                     GRIEIGSHTLTHINMGTTSAADKRHELAESRRLLQAQTGQAVASFAYPFGIYGEEDVA
                     LARAAGYTTAVTTIDGIDARTPRPDPLQLKRIKISGKDKLLAFALRMRGGKRGWKK"
     gene            1711..2706
                     /locus_tag="PKN25_14755"
     CDS             1711..2706
                     /locus_tag="PKN25_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HNV92210.1"
                     /translation="MEEVRPLAIWLLSDGAPGHLSQSRGVVDALATRRMVETRTIELK
                     IRSPFWKRLGRLLLPRIRDTDTWLRRIYGLTPPAGQPALIVSSGANTLLANALLARRH
                     GVPNVYSGTLKGYDPAAFAGVFTVVPLGVACNHVLPLPPVPGELARPLPPPTGEPRIA
                     VLVGGDGAGYVFKEADWRAFGAGLAALARRDGARLLLTTSRRTGAVAEAWLRESIPVE
                     LIADAVWWSQAPRKVMRDFLGAASAIVVTEDSLSMVAESLYSGRPVVSVSPVVAQPNA
                     NDGAALQAYAERGLLVRQPLAGLAYIAFPDCSTPVPDVQAEIADVVLALLEPTTP"
     gene            2703..>3598
                     /locus_tag="PKN25_14760"
     CDS             2703..>3598
                     /locus_tag="PKN25_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV92211.1"
                     /translation="MKKVLIWGRYGNYGPDYPRNRVIESVLRSLGCEVSRFLPALSAT
                     ADLEYALRNLLERSHRPDLVWVPCFRQRDLAAAARYARRQRVPLVFDPLISAYDKQVN
                     EKHKFAADSAKARKLLEWESRLFQLPDWLIADTEGHADYFHATHGVARARIRVIPVGA
                     EESLFTPQPWPHKPADAPLELAFFGTFIGLQGVDVLAQAILHYDGPPTHWRLIGEGPM
                     KAECERLLAPLAGATGPSRVSFEGWGPLPELPGRLASADAILGIFGTSDKALRVIPNK
                     VYQGLAIGRAVLTAATPAFTPEL"
BASE COUNT          541 a         1346 c         1197 g          514 t
ORIGIN      
        1 tgcgcaggct cttcgaggcc gagcgcttcg acatcgtcca tgcccgctcg cgcctgcccg
       61 cctgggtggc ttggctggcc tggcgcggca tgccggctga cgcccgccca cacttcgtca
      121 ccacggtgca cggcatgcac tcggtcagcc gctacagcgc catcatgtgc gcgggcgagc
      181 gcgtgatcgc agtcagcgac acggtgcgcg actacctccg cagccactac ccgccgacac
      241 gctggccgca tctcgcggac gagcgcatca cggtgatccc gcgcggcatc gacccggcgg
      301 agtttccgcg cgactatccg ccgtcggacg aatggctggc gcgctttcat gccgaattcc
      361 cgcagatcgg ccagcgcaag gtgctgacgc tgcccgggcg gctgacgcgc ctgaagggtc
      421 atcacgactt catcaccctg gtcgccaggc tggtcgccga gggcatcgac gtgatcgggc
      481 tggtcgtcgg cggcgaagac ccgaagcggc ccggctacgc gaaggagatc cgcgagcgcg
      541 tgcaggcgga ggggctgggc gatcgcatcg tgttcaccgg ccaccgcagc gacgtgcgcg
      601 agatctattc ggtttcggat tgcgtgctga gcttgtcgag cacgccggaa tccttcgggc
      661 gcacggtgct cgagccgctg gcgatggggc ggccggtggt ggggtacgcg catggcgggg
      721 tggcggagat cctcgccgag ctgttcccgc atggggcggt gggcaagggg gacgtcgccg
      781 ccgctaccgt gcgggcggcg gacgtgatcg ccggacggac cccggtggtg gagcacaaca
      841 gccgcttcct gctcgcgaac atgcaggcgc agacgctgaa gctttacagg agcctcgcat
      901 gaccgagtgg ctcgccctct ccgcggcgct cgccgccggc tggttctcca accgctacgc
      961 ctggtggcgc aagccggtcg atctgcgcca tccgcgcgtg ctgatgtacc acatggtcag
     1021 cccgcaccgc ccgggcgcga agttcaacgg cctgcgcgtc ccccccgaac gcttcgaggc
     1081 ccagctcgac tggctgaagc gcaagggctg gacatttttc accgtcggcg agctgtgggc
     1141 gcagtgggac gagctgcccg agaagagtgt cgcgatcacc ttcgacgacg gctacgccga
     1201 caacctgcat aacgccctgc ccttgctgga caagtacgac gccaaggcaa cgatctacat
     1261 cgtcgtcgac cgccacgacc gcgactggtc caccgccaag aaggcgcacc acaacaccgg
     1321 cgagctcgcg cgcgaaccca agctgagcga cgacgagctg cagcgcctgg tcgcgagcgg
     1381 ccgcatcgag atcggctcgc acacgctcac ccacatcaac atgggcacca cttcggcggc
     1441 agacaagcgc cacgagctgg ccgagtcccg ccgcctgctg caggcgcaga ccggccaggc
     1501 ggtggcgagc ttcgcctatc cgttcgggat ctacggcgag gaagacgtcg cgctggcgcg
     1561 cgcggccggc tacacgaccg cggtgacgac catcgacggc atcgacgcac gcaccccgcg
     1621 ccccgatccg ctgcagctca agcgcatcaa gatcagtggc aaggacaagc tgctcgcgtt
     1681 cgcgctgcgc atgcgcggcg ggaaacgggg gtggaagaag tgaggccgct cgccatctgg
     1741 ctgctcagcg acggggcccc cgggcacctc agccagagcc gcggcgtcgt cgatgcgctc
     1801 gccacccggc ggatggtgga aacgcgcacc atcgagctga agatccgcag ccctttctgg
     1861 aagcgcctgg gccgcctgct gctgccgcgc atccgcgaca ccgacacgtg gctgcggcgg
     1921 atctacggcc tcaccccacc cgccggccag cccgcgctga tcgtctcgtc cggcgccaac
     1981 accctgctcg ccaacgccct gctcgcgcgc cggcacggcg tgcccaacgt gtatagcggc
     2041 accctcaagg gctacgaccc ggccgccttc gccggcgtgt tcaccgtggt gccgctcggt
     2101 gtcgcgtgca atcacgtgct gcccctgccg ccggtgccgg gcgagctcgc ccgtccgctg
     2161 cctcccccga ccggcgaacc acggatcgcg gtactcgtcg gcggcgatgg cgctggctat
     2221 gtcttcaagg aagccgactg gcgcgccttc ggcgccggcc tcgccgcgct cgcccgccgc
     2281 gacggcgccc gcctgctgct caccacctcc cgccgtaccg gcgctgtcgc cgaagcctgg
     2341 ctgcgcgaaa gcatccccgt cgaactgatc gccgacgccg tgtggtggtc gcaggcgccg
     2401 cgcaaggtca tgcgcgattt cctcggcgcg gcaagcgcga tcgtcgtcac cgaggacagc
     2461 ctcagcatgg tggccgagag cctctactcc ggccggccgg tggtctcggt ctcgcccgtg
     2521 gtcgcccagc ccaacgccaa cgatggcgcc gcgctgcagg cttatgcaga gcgcggcctg
     2581 ctggtgcgcc agccgctcgc gggcctcgcc tacatcgcat tccccgactg cagcacgccc
     2641 gtgcccgacg tgcaggccga gatcgccgac gtcgtcctcg cgctgctgga gccgacaaca
     2701 ccatgaagaa ggtgctgatc tggggccgct acggcaacta cgggcccgac tacccgcgca
     2761 accgcgtcat cgagtcggtg ctgcgcagcc tcggctgcga ggtgagccgc ttcctgcccg
     2821 cgctgtcggc caccgccgac ctcgagtacg ccctgcggaa cctcctggag cgcagccacc
     2881 gccccgacct cgtctgggtg ccgtgcttcc gccagcgcga cctcgccgcc gccgcgcgct
     2941 acgcccgccg ccagcgcgtg ccgctggtct tcgacccgct gatcagcgcc tacgacaagc
     3001 aggtgaacga aaagcacaag ttcgccgcgg acagcgcaaa ggcgcgcaag ctgctggagt
     3061 gggaatcgcg cctcttccag ctgcccgact ggctgatcgc cgacaccgag ggccacgccg
     3121 actacttcca cgccacccac ggcgtggcgc gcgcgcgcat ccgcgtgatc ccggtcggcg
     3181 ccgaggagtc gctgttcacc ccgcagccct ggccgcacaa gcccgccgat gcgccgctgg
     3241 aactcgcctt cttcggcacc ttcatcggcc tgcagggggt ggatgtgctg gcgcaggcca
     3301 tcctgcacta cgacggcccg cccacccact ggcgcctgat cggcgaaggg ccgatgaagg
     3361 cggagtgcga acgtctcctc gcgccgcttg ccggcgccac cggccccagc cgcgtcagct
     3421 tcgaaggctg gggcccgctg cccgagctcc ccggccggct cgccagcgcc gacgccatcc
     3481 tcggcatctt cggcaccagc gacaaggcgc tgcgggtgat tccgaacaag gtgtatcagg
     3541 ggctggcgat cgggcgggcg gtgctcaccg cggcaacgcc ggccttcacg cccgaact
//
LOCUS       DAOQBS010000205         3546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_29369_length_3546_cov_18.473790, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000205 DAOQBS010000000
VERSION     DAOQBS010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29369_length_3546_cov_18.473790"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PKN25_14765"
     CDS             complement(<1..871)
                     /locus_tag="PKN25_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV92212.1"
                     /translation="MRKKIPSTAALLAFEAAARHESFTRAAEELALTQSAICRQIGAL
                     EDFLGVPLFRRTRRGVQLTEAGLSYSRQIAPRLDAIEHDTLAVMAHHGAGSVLELAVV
                     PTFATRWLLPRLAGFQARNPDVVVNMHTQTRPFLFDQTEFDAAIYFGDTGWPGTEAHF
                     LMREYPVPVCSPALPGVREQMSPDEVAELPLLQQTTRPYAWRQWFASAGVRPANDMGG
                     MRLELFSMLAQAAIERMGVALIPPFLIQQELAAGSLISPCLRSFPSTRAYYLIVPEGK
                     AERPALTNFREWLV"
     gene            1014..2201
                     /locus_tag="PKN25_14770"
     CDS             1014..2201
                     /locus_tag="PKN25_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNV92213.1"
                     /translation="MASNKASFNWEDPLLLDLQLTETERMVRDTARAYSQDKLLPRVQ
                     EAFRHEKTDAAIFREMGELGLLGATIPEQYGGSGLNYVCYGLIAREVERVDSGYRSMM
                     SVQSSLVMVPINEFGTEAQKQKYLPKLATGEWIGCFGLTEPNHGSDPGSMITRARKVD
                     GGFSLSGSKMWITNSPIADVFVVWAKDDEGQIRGFILEKGWKGLSAPAIHGKVGLRAS
                     ITGEIVMDEVFVPEENAFPTVRGLKGPFTCLNSARFGIAWGALGAAEACYETARQYTM
                     DRNQFGRPLAANQLIQKKLADMLTEITLGLQAVLRVGRMKDEGIAPVEITSIIKRNSC
                     GKALDIARMARDMLGGNGISDEYPVARHLVNLEVVNTYEGTHDVHALILGRAITGLAA
                     FSN"
     gene            2219..3442
                     /locus_tag="PKN25_14775"
     CDS             2219..3442
                     /locus_tag="PKN25_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNV92214.1"
                     /translation="MPGALSHIRVLDLSRILAGPWAGQMLADLGADVIKVERPGAGDD
                     TRGWGPPWLKDEQGADTDVAAYYLCANRNKRSITIDITQAEGQALVRRLAAESDVVLE
                     NFKVGGLAQYGLDYDSLKAVNPRLVYCSITGFGQDGPYAPRAGYDFLIQGLGGLMSIT
                     GRPDGEEGGGPMKVGVALTDILTGLYATNAVLAALAWRERSGEGQYIDMALLDVQVAC
                     LANQAGNYLATGQSPKRLGNAHPNIVPYQDFPTADGYMILAIGNDGQFARFCAAAGAP
                     QLATDERFATNRARVVNRATLIPLLKKLTVERGTAEWIAKLEALAVPCGPINTLADVF
                     ADPQVQAREMKVTMPHPVAGQVPLVASPMKLSATPVDYRLPPPMLGEHTDEILAATLG
                     LDAGAIARLRADGVV"
BASE COUNT          597 a         1242 c         1196 g          511 t
ORIGIN      
        1 cgaccagcca ttcgcggaag ttcgtcagcg ccggccgttc cgccttgccc tcgggcacga
       61 tcaggtagta cgcccgcgtg ctcgggaagc tgcgcaggca ggggctgatc aggctgccgg
      121 cggcgagctc ctgctggatc aggaagggcg ggatcagcgc cacgcccatg cgctcgatcg
      181 ccgcctgcgc gagcatcgag aacagctcca ggcgcatgcc gcccatgtcg ttcgcggggc
      241 gcacgccggc cgaggcgaac cactgccgcc aggcgtaggg gcgggtggtc tgctgcagca
      301 gcggcagctc ggcgacctcg tccgggctca tctgctcgcg cacgccgggc agggccgggc
      361 tgcataccgg caccggatat tcccgcatca ggaagtgcgc ctcggtgccc ggccagccgg
      421 tgtcgccgaa gtagatcgcg gcgtcgaact cggtctggtc gaagaggaac ggccgcgtct
      481 gggtgtgcat gttcaccacc acgtccgggt tccgcgcctg gaagccggcc aggcggggca
      541 gcagccagcg cgtggcgaag gtcggcacca ccgcaagttc gagcacgctg ccggcgccgt
      601 ggtgtgccat gaccgcgagc gtgtcgtgct cgatcgcatc cagccgcggg gcgatctggc
      661 ggctgtagct cagtcccgcc tcggtgagct gcacgccgcg ccgggtgcgg cggaacagcg
      721 gcacgccgag gaagtcctcg agcgcgccga tctgccggca gatcgcgctc tgcgtgagcg
      781 cgagctcctc ggcggcgcgg gtgaagctct cgtggcgcgc ggcggcctcg aaggcgagca
      841 gcgcggcggt gctggggatc ttcttgcgca tggcggtcga gattttcttt cggagttccg
      901 gaaaggcaca gattactgca gaaatatcgc ttgagctgcg cttccggagt tcataggatg
      961 cgaatcacac acaggcagcc gcctgcacga ccgatcgcac gaggagacac gacatggctt
     1021 cgaacaaggc tagcttcaac tgggaagacc cgctgctgct cgacctgcag ctgaccgaga
     1081 ccgagcgcat ggtgcgcgac acggcgcgcg cctacagcca ggacaagctg ctgccgcgcg
     1141 tgcaggaggc cttccgccac gagaagaccg atgccgcgat cttccgcgag atgggcgaac
     1201 tcggtctgct cggcgccacc atccccgagc agtacggcgg ctccggcctc aactacgtgt
     1261 gctacggcct gatcgcccgc gaggtcgagc gtgtcgactc cggctaccgc tcgatgatga
     1321 gcgtgcagag ctcgctggtg atggtgccga tcaacgaatt cggtaccgag gcgcagaagc
     1381 agaagtacct gcccaagctc gccaccggtg aatggatcgg ctgcttcggc ctcaccgagc
     1441 cgaaccacgg ctccgacccg ggctccatga tcacccgtgc gcgcaaggtc gatggcggct
     1501 tcagcctctc cggcagcaag atgtggatca ccaacagccc gatcgccgac gtgttcgtgg
     1561 tgtgggccaa ggacgacgaa ggccagatcc gcggcttcat cctcgagaag ggctggaagg
     1621 gcctgtccgc gcccgccatc cacggcaagg tcggcctgcg cgcctcgatc accggcgaga
     1681 tcgtcatgga cgaggtcttc gtgccggaag agaacgcctt cccgaccgtg cgcgggctca
     1741 agggcccctt cacctgcctc aactcggcgc gcttcggcat cgcctggggt gcgctcggcg
     1801 ccgccgaggc ctgctacgag accgcccgcc agtacacgat ggaccgtaac cagttcggcc
     1861 gtccgctcgc cgccaaccag ctcatccaga agaagctcgc cgacatgctg accgagatca
     1921 ccctcggcct gcaggcggtg ctgcgcgtgg gccgcatgaa ggacgagggc atcgccccgg
     1981 tcgagatcac ctcgatcatc aagcgcaact cctgcggcaa ggcgctcgac atcgcccgca
     2041 tggcgcgcga catgctcggc ggcaacggca tctcggacga gtacccggtg gcccgccacc
     2101 tggtgaacct cgaggtggtc aacacctacg agggcaccca cgacgtgcat gcgctgatcc
     2161 tcggccgcgc catcaccggc ctcgccgcgt tcagcaactg agtccccgga gacccccgat
     2221 gcccggcgcc ctgtcgcaca tccgcgtcct cgacctgtcc cgcatcctgg ccggcccctg
     2281 ggccgggcag atgctcgccg atctcggcgc ggacgtgatc aaggtcgagc gtccgggtgc
     2341 cggcgacgac acccgcggct gggggccgcc ctggctcaag gacgagcagg gcgccgacac
     2401 cgacgtggcg gcgtattacc tgtgcgccaa ccgcaacaag cgctcgatca ccatcgacat
     2461 cacccaggcc gagggccagg cgctggtgcg ccggctcgcc gccgagtccg acgtggtgct
     2521 ggagaacttc aaggtcggcg gcctggcgca gtacggcctc gactatgaca gcctgaaggc
     2581 ggtcaatccg cgcctggtgt actgttcgat caccggcttc ggccaggacg ggccgtatgc
     2641 gccgcgcgcc ggctacgact tcctgatcca gggcctgggc gggctgatga gcatcaccgg
     2701 ccggcccgac ggcgaggagg gcggtggccc gatgaaggtg ggcgtggcgc tcaccgacat
     2761 cctcaccggc ctgtacgcca ccaacgccgt gctcgccgcg ctggcctggc gcgagcgcag
     2821 cggcgaaggc cagtacatcg acatggcgct gctcgacgtg caggtggcct gcctcgccaa
     2881 ccaggcgggc aactacctcg ccaccgggca gagcccgaag cggctgggca atgcgcaccc
     2941 caacatcgtg ccctaccagg attttccgac cgccgacggc tacatgatcc tcgccatcgg
     3001 caacgacggg cagttcgcgc gcttctgcgc ggccgccggt gcgccgcagc tcgcgaccga
     3061 cgaacgcttc gccaccaacc gcgcgcgcgt ggtcaatcgc gccacgctga tcccgctgct
     3121 gaagaagctc accgtcgagc gcggcaccgc ggagtggatc gcgaagctcg aggcgctcgc
     3181 cgtgccctgc ggtccgatca acaccctggc cgacgtcttc gccgacccgc aggtgcaggc
     3241 gcgcgagatg aaggtgacga tgccgcatcc ggtcgccggc caggtgccgc tcgtcgccag
     3301 cccgatgaag ctctcggcca cgccggtgga ctaccgcctg ccgccgccga tgctcggcga
     3361 gcacaccgac gagatcctcg ccgcaacgct cggtctcgat gccggggcga tcgcccgcct
     3421 gcgtgcggac ggcgtggtct gagccggaac aagaacacag aagacgaaca caagggaaca
     3481 gcatgaagat cctcgtaccc gtaaagcgcg tggtggatta caacgtcaag gttcgcgtga
     3541 aggccg
//
LOCUS       DAOQBS010000206         3527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_29583_length_3527_cov_17.271889, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000206 DAOQBS010000000
VERSION     DAOQBS010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29583_length_3527_cov_17.271889"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PKN25_14780"
     CDS             <1..836
                     /locus_tag="PKN25_14780"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HNV92215.1"
                     /translation="VNTIDEHLDMLMVCHHLDPSIAEDVAFAESRIRRETIAAEDILH
                     DTGAFSMMSSDSQAMGRVGEVVIRTWQTAHKMKLQRGWLAPRRSAAAAVPDAVAVVEG
                     STANDNFRVKRYIAKYTINPALTHGIAHEVGSIEPGKLADLVLWRPAFFGVKPSLVIK
                     GGMIAAAAMGDPNASIPTPQPVHWRPMFGSFGRALKCAVTFVSQAALHNAAVAALGLQ
                     KPLVAVKGCRTLTKADMVLNNATPQIEVDPETYVVRADGEHLACEPATELPLAQRYFL
                     F"
     gene            874..1422
                     /gene="ureE"
                     /locus_tag="PKN25_14785"
     CDS             874..1422
                     /gene="ureE"
                     /locus_tag="PKN25_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HNV92216.1"
                     /translation="MPMTAETLADFVMPAPATDVLLIEQFHEGDEPASEVLELDFGAR
                     TKSRLRTRLASGAEVGLFLPRGTILRGGQKLRARDGRIVEVRAAAEDLLEARCASAVA
                     LARAAFHLGNRHVAVEVGHDEAGDWLRLQADHVLEGMLVGLGALVSALRAPFEPEAGA
                     YAPGHQHPGDGSGARIHLMSGQ"
     gene            1419..2198
                     /locus_tag="PKN25_14790"
     CDS             1419..2198
                     /locus_tag="PKN25_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HNV92217.1"
                     /translation="MSAATHRPMPAPARPCAAAAAVIECNAAGAGRGLLPLVRLLQLA
                     SPALPVGAYTYSQGLEWAVESGLVRSEAEAAAWIGELLEWSLARFEVPLLGCQLAAWS
                     RNEDAELARLNDDFLASRETAELRAETVQMGWSLVRLLAELDAFASIPGWRARLLAIE
                     APAFPTAWSAAAAAWQVAPGEALVAYLWAWLENQVMAAVKAVPLGQSAGQRLLATLGD
                     RIPALAAAALVLPETAWSNYTPGLAIASSRHETQYTRLFRS"
     gene            2195..2872
                     /gene="ureG"
                     /locus_tag="PKN25_14795"
     CDS             2195..2872
                     /gene="ureG"
                     /locus_tag="PKN25_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HNV92218.1"
                     /translation="MTPISALHAAHAPTTARAHAGPQRGPLRVGIGGPVGSGKTALTL
                     AMCLALRDKYDIAVVTNDIYTAEDAQFLVRNEALAPERIIGVETGGCPHTAIREDASI
                     NLEAVERLNARFPGLDIVFVESGGDNLAATFSPELSDLTIYVIDVSAGDKIPRKGGPG
                     ITRSDLLVINKIDLAPLVGASLEVMDRDARRMRGDRPFLFSNQKTGQGLREIIDFIER
                     QGMLAAA"
     gene            2911..3492
                     /locus_tag="PKN25_14800"
     CDS             2911..3492
                     /locus_tag="PKN25_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNV92219.1"
                     /translation="MTIRFRSVLAASALASLSGAALAHPGHAPASFASGFAHPLGGLD
                     HLLAMLAVGLYAARQAGAARLLLPTGFVLAMLAGAGLGALGLALPAVEAGISASVLVL
                     GLLIAFAARLPLAASLPLVAAFALFHGHAHHAEMGDATLLGYSLGFALATAALHAAGL
                     TLARAFPDSRGGRLALRLGGGGIAGVGVALLGG"
BASE COUNT          488 a         1352 c         1202 g          485 t
ORIGIN      
        1 cggtcaacac catcgacgag cacctcgaca tgctgatggt gtgccaccac ctcgatccga
       61 gcatcgccga ggacgtggcc ttcgccgaat cgcgcatccg ccgcgagacc atcgccgccg
      121 aggacatcct gcacgacacc ggcgcgttct cgatgatgtc gtccgactcg caggccatgg
      181 gccgggtggg cgaggtggtg atccgcacct ggcagaccgc gcacaagatg aagctgcagc
      241 gcggctggct ggcgccgcgg cgcagcgcgg cggcggcggt gccggacgcg gtggccgtgg
      301 tcgagggcag caccgccaac gacaacttcc gcgtcaagcg ctacatcgcc aagtacacga
      361 tcaaccctgc gctgacccac ggcatcgccc acgaggtggg ctcgatcgag ccgggcaagc
      421 tcgccgacct ggtgctgtgg cgcccggcct tcttcggcgt caagccgagc ctggtgatca
      481 agggcggcat gatcgccgcg gccgcgatgg gcgaccccaa cgcctcgatc ccgaccccgc
      541 agccggtgca ttggcggccg atgttcggca gcttcggacg cgcgctgaaa tgcgcggtga
      601 ccttcgtgtc gcaggccgcg ctgcacaacg ccgcggtggc cgcgctcggg ctgcagaagc
      661 cgctggtggc ggtgaagggc tgccgcacgc tgacgaaggc cgacatggtg ctcaacaacg
      721 ccacgccgca gatcgaggtc gatcccgaga cctatgtcgt gcgcgccgac ggcgagcacc
      781 tcgcctgcga acccgccacc gagctgccgc tggcgcaacg ctatttcctg ttctgagcgc
      841 agcgcaccgg aaccgcactg gagacctccc gccatgccga tgaccgcaga aaccctggcc
      901 gacttcgtga tgcccgctcc cgccaccgac gtgctgctga tcgagcagtt ccacgagggc
      961 gacgagccgg cgagcgaggt gctcgagctc gacttcggcg cccgcaccaa gagccgcctg
     1021 cgcacccggc tcgcctccgg cgccgaggtg ggcctcttcc tgccgcgcgg caccatcctg
     1081 cgcggcggac agaagctgcg cgcacgcgac ggccgcatcg tcgaggtgcg cgccgccgcc
     1141 gaggacctgc tcgaggcgcg ctgcgcctcg gcggtagcgc tggcgcgcgc cgccttccac
     1201 ctcggcaacc gccacgtcgc ggtcgaggtc gggcacgacg aggccggcga ctggctgcgc
     1261 ctccaggccg atcacgtgct cgaaggcatg ctggtggggc tgggcgcact ggtgagcgcg
     1321 ctgcgcgcgc ccttcgagcc cgaggccggc gcctatgcgc ccggccacca gcacccgggc
     1381 gacggcagcg gcgcgcgcat ccacctcatg agcgggcaat gagcgccgcc acccatcgcc
     1441 cgatgccggc gccggcaagg ccatgcgccg ccgcagccgc tgtcatcgaa tgcaacgccg
     1501 cgggagcggg tcgcggcctg ttgccgctgg tgcgcctgct gcagctcgcc agcccggcac
     1561 tgcccgtcgg cgcctacacc tactcgcaag gcctggagtg ggcggtggag agcggcctcg
     1621 tgcgcagcga ggcggaggcc gcggcgtgga tcggcgagct gctggaatgg agcctggcac
     1681 gcttcgaggt gccgctgctc ggttgccagc tcgcggcgtg gtcgcgaaac gaagacgccg
     1741 agctcgcgcg gctcaatgac gactttctcg ccagccgcga gactgccgag ctgcgcgccg
     1801 agacggtgca gatgggctgg tcgctggtga ggctgctggc cgagctggat gcgtttgcgt
     1861 cgattcccgg ctggcgcgcg cgcctgctgg cgatcgaggc ccccgccttt cccaccgcgt
     1921 ggtcggccgc cgccgcggcc tggcaggtag cgcccggcga ggcgctcgtc gcctacctgt
     1981 gggcctggct ggagaaccag gtgatggccg cggtgaaggc cgtgccgctc ggccagagtg
     2041 ccgggcagcg cctgctcgcc acgctcggcg accgcatccc ggcgctggcg gcggcggcgc
     2101 tcgtgctgcc cgagacggcc tggagcaact acacccccgg cctggcgatc gccagcagcc
     2161 ggcacgagac ccaatacacg aggctgttcc gatcatgacc ccgatttctg ccctccacgc
     2221 cgcgcatgcc cccacgaccg cccgtgcaca cgccggaccg cagcgcggcc ccctgcgcgt
     2281 cggcatcggc ggcccggtcg gttcgggcaa gaccgcgctg accctggcga tgtgcctggc
     2341 cctgcgcgac aagtacgaca tcgccgtggt caccaacgac atctacaccg ccgaggacgc
     2401 ccagttcctc gtgcgcaacg aggcgctcgc gcccgagcgc atcatcggcg tggagaccgg
     2461 cggctgcccg cacaccgcga tccgcgagga cgcctcgatc aacctcgagg cggtcgagcg
     2521 cctgaacgcg cgcttccccg ggctggacat cgtcttcgtc gaatccggcg gcgacaacct
     2581 cgccgccacc ttctcgcccg agctctccga cctgacgatc tacgtgatcg acgtctccgc
     2641 cggcgacaag atcccgcgca agggcgggcc gggcatcacc cgcagcgacc tgctggtgat
     2701 caacaagatc gacctcgcgc cgctggtagg cgcctcgctc gaggtcatgg accgcgacgc
     2761 gcgcaggatg cggggagatc gccccttcct gttctcgaac cagaagaccg gacagggcct
     2821 gcgtgagatc atcgacttca tcgagcgcca gggcatgctg gccgccgcct gaacaccgca
     2881 agaccttccc gcactcctgg agaatccacc atgacgatcc gcttccgctc cgtgctcgcc
     2941 gccagtgcgc tcgcaagcct ctcgggcgcc gcgctcgccc accccgggca cgcacccgcc
     3001 agcttcgcca gcggcttcgc ccatccgctc ggcgggctcg accacctgct cgccatgctc
     3061 gcggtcggcc tctacgccgc acgccaggcg ggcgctgcac gcctgctgct gcccaccggc
     3121 ttcgtgctgg cgatgctcgc cggcgccggc ctcggcgcgc tcggcctcgc cctgcccgcg
     3181 gtcgaggccg gcatttccgc ctcggtgctg gtgctgggcc tgctgatcgc attcgccgcc
     3241 cgcctgccgc tggcggcgag cctgcccctg gtcgccgcct tcgcgctctt ccacggccat
     3301 gcccaccacg ccgagatggg cgacgccacc ctgctcggct acagcctcgg cttcgcactc
     3361 gcgaccgcgg ccctgcacgc ggccggcctg acgctcgcgc gcgccttccc cgacagccgc
     3421 ggcggacgcc ttgcgctgcg cctgggcggc gggggaatcg ccggcgtggg cgtcgccctg
     3481 ctcggcggct gaagcctccg cggcaagacc gccgcgccgg tgctagg
//
LOCUS       DAOQBS010000207         3513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_29766_length_3513_cov_14.861191, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000207 DAOQBS010000000
VERSION     DAOQBS010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29766_length_3513_cov_14.861191"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1123)
                     /gene="lptG"
                     /locus_tag="PKN25_14805"
     CDS             complement(47..1123)
                     /gene="lptG"
                     /locus_tag="PKN25_14805"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNV92220.1"
                     /translation="MSPVLLRYLAREIFAASALVLVAFLGLFAFFDFINELEDVGKGG
                     YTLYQALVYVGLTLPARVYELMPIAVLIGTLYALATLAKNSEITVMRASGMSNALLTS
                     VLGLIGGVFAVLIFLVGEYVAPPAEAAAEEWKLTATQATVSQQLRSGLWVKDGRLVLN
                     VRTLMPDRSMRGARIYAFDEDYALESIAEAATGVFDGSGEWKLLDVQRTRFHGDRTEV
                     ERLPEIRWRSELTPEVLGVLMVSPEHMPFGRLWTYINHLRDNNQNADRFEIALWKKLI
                     YPFTALVMMALAMPFGFIHDRMGGASARIFMGVMLGVGFHLLNGLFSNLGMINAWPPF
                     MAALTPSLIFLAAAFWLMHRVERR"
     gene            complement(1203..2285)
                     /gene="lptF"
                     /locus_tag="PKN25_14810"
     CDS             complement(1203..2285)
                     /gene="lptF"
                     /locus_tag="PKN25_14810"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HNV92221.1"
                     /translation="MIFRRALQREFAQTAAAVFVALFAILISTVLIRLLGQAAGGRVP
                     PDAVVALIGFGALAQLPTVLTLTLFIAILVSLSRSYRDSEMVVWFASGVPLTAWIRPV
                     LMFALPVVLVAGGGSLYLTPWSQQKSAEYRERLENRDDTHRVAPGVFRESASAQRVFF
                     VEVGAGEDGRVRNVFVSEQTRSGQLVVIASAEGYLRGDADGRRFVVLEKGRRYDGTPG
                     TPEYRVMEFDRYEVQVEQKQVAGQQARIRALPTAVLLAQPEPRGLGELVGRFGVPAVA
                     LLLALMAIPLSFVNPRAGRANNLLVALLVYLLYSNAISVFQSWVAQGKLPFVAGLLLP
                     HVVVLAVLALMFHKRLAISPFWRRRA"
     gene            2424..>3513
                     /locus_tag="PKN25_14815"
     CDS             2424..>3513
                     /locus_tag="PKN25_14815"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNV92222.1"
                     /translation="MEFTIKTGSPAKLKTGLLVLGAFAEGRLPALSAAADGAAEGRLA
                     ALIKRGDLEDKAGATLLVHDLPGVHAERVLLVSLGKHDEFGDKAYRDALAAAAKAISA
                     GPAKDAVVALADAELSGRDMAWRLQQAARILADGAYRFDALKSDKKTRKERGAKKLCL
                     LVSCELGAELDTAVLQGHAIASGMALAKDLGNLPGNHCTPTHLAETAESLGKQYKFDV
                     EVLERDDMEKLGMGSFLSVARGSHQPPKFIVMHYKGGKAKAKPVVLVGKGITFDTGGI
                     SLKPAAEMDEMKFDMCGAASVLGTFKAVAQMGLPINLVGLVPTTENMPGGGATKPGDV
                     VTSMSGQTIEVLNTDAEGRLILCDALTYA"
BASE COUNT          640 a         1228 c         1176 g          469 t
ORIGIN      
        1 gcgacgcctg gacacgcgcc gggacgcctc ctccgtggcc ccctgctcag cgccgttcga
       61 cgcgatgcat gagccagaac gcggccgcga ggaagatcag gctgggcgtc agcgcagcca
      121 tgaagggcgg ccaggcgttg atcatgccga ggttggagaa caggccgttg aggaggtgga
      181 agcccacgcc cagcatcacc cccatgaaga tgcgcgcgct cgcgccgccc atgcggtcgt
      241 ggatgaaacc gaagggcatc gccagcgcca tcatcaccaa cgcggtgaag gggtagatca
      301 gcttcttcca cagcgcgatc tcgaagcggt cggcgttctg gttgttgtcg cgcaggtggt
      361 tgatgtaggt ccacaggcga ccgaagggca tgtgctcggg cgacaccatg agcaccccca
      421 gcacctccgg cgtcagctcc gaacgccagc ggatttcggg caggcgctcg acctcggtgc
      481 gatcgccgtg gaagcgcgta cgctgcacgt cgagcagctt ccactccccg ctgccgtcga
      541 acacgccggt cgccgcttcg gcgatcgatt cgagcgcgta gtcttcatcg aaggcgtaga
      601 tgcgcgcgcc gcgcatgctg cggtcgggca tcagcgtgcg cacgttgagc acgagccggc
      661 cgtccttcac ccacaggccc gagcgcagtt gctgcgagac ggtcgcctgc gtggcggtga
      721 gcttccactc ctccgcggcg gcctccgcgg gcggcgccac gtattcgccg accaggaaga
      781 tcagcacggc gaacactccg ccgatcaggc cgagcacgct cgtcagcagc gcgttcgaca
      841 tccccgaggc tcgcatcacg gtgatctcgg agttcttcgc caaggtcgcc agcgcataca
      901 gggtgccgat cagcaccgcg atcggcatca gctcatacac ccgcgccggc agggtgagtc
      961 cgacatacac cagggcctgg tagagcgtgt agccgccctt gcccacgtcc tcgagctcgt
     1021 tgatgaagtc gaagaaggcg aacaggccga gaaaggccac cagcaccagc gcactggcgg
     1081 cgaagatctc gcgcgcgaga tagcgcagca gaaccggact caccggactc ccctcctgcc
     1141 gccgggcgac atccttcggg agggcgccga gcgcctccct tcgggcggcc gtgcgggtgc
     1201 gctcatgcac gcctccgcca gaacggcgag atcgccaggc gcttgtggaa catcagcgcc
     1261 agcaccgcca gcaccaccac gtggggcagc agcaatccgg ccacgaaggg cagcttgccc
     1321 tgcgcaaccc aggactggaa cacggaaatc gcgttgctgt agagcaggta cacgagcagc
     1381 gccaccagca ggttgttcgc acgcccggcg cgcggattga cgaaggacag cgggatcgcc
     1441 atcaaggcca gcagcaacgc gaccgccggc acgccgaagc ggccgacgag ttcgccgagg
     1501 ccgcgcggct cgggctgcgc gagcagcacc gcggtcggaa gcgcgcggat gcgtgcctgc
     1561 tggcccgcga cctgcttctg ctcgacctgc acttcgtagc gatcgaactc cattacccgg
     1621 tactcgggcg tacccggggt accgtcgtag cgccgccctt tctcgagcac gacaaagcgc
     1681 cggccgtccg catcgccgcg caggtagcct tcggccgagg cgatcaccac caactggccg
     1741 ctgcgggtct gctcgctgac gaagacattg cgcacccggc catcctcgcc ggcgccgacc
     1801 tcgacgaaga acacccgctg cgcgctcgcg gactcgcgga agacgccggg cgcgacgcga
     1861 tgggtatcgt cgcgattctc caggcgctcg cggtattccg cgctcttctg ctgtgaccac
     1921 ggcgtcaggt agagcgagcc gccaccggcc accagcacga cgggcagcgc gaacatcagg
     1981 accggccgga tccaggcggt gaggggcaca cccgaggcga accacaccac catctcggaa
     2041 tcccggtagc tgcgcgacag cgacaccagg atggcgatga acaaggtgag cgtcagcacc
     2101 gtgggcagct gcgcaagcgc gccgaacccg atcagcgcca ccacggcatc gggcggcacg
     2161 cgcccgcccg ccgcctggcc gagcaggcgg atgagcacgg tggagatcag gatcgcgaac
     2221 agcgccacga agaccgcggc ggccgtctgc gcgaattccc gttgcagggc gcgccggaag
     2281 atcatcgggc gggggtcgat accgggatga ggccgggcgg caggccgcgg cggatgtgga
     2341 tgggaagcgt ttttgacgcc ccggttgcga acgcgccata atcttcggca atcgaaacca
     2401 ggcatccaaa caaggaaaaa cgcatggaat ttaccataaa gaccggcagc ccggccaagc
     2461 tcaagactgg cctgctggta ctcggcgcgt ttgccgaagg ccgtctcccc gccctctcgg
     2521 ccgcggccga cggtgctgcc gaaggccgcc tggccgccct gatcaagcgt ggcgacctcg
     2581 aggacaaggc gggcgccacg ctgctggtgc atgacctgcc cggcgtgcac gccgagcgcg
     2641 tgctgctggt gagcctgggc aagcacgacg agttcggcga caaggcgtac cgcgacgcgc
     2701 tcgccgccgc ggcgaaggcg atctccgccg ggccggcgaa ggacgccgtg gtcgcgctcg
     2761 ccgacgcaga gctctccggt cgcgacatgg cgtggcggct gcagcaggcc gcgcgcatcc
     2821 tcgccgacgg cgcctaccgc ttcgacgcac tcaagtccga caagaagacc cggaaggagc
     2881 gtggcgcgaa gaagctctgc ctgctggtga gctgcgagct cggcgccgag ctcgacaccg
     2941 ccgtcctgca gggccacgcg atcgccagcg gcatggcgct ggcgaaggac ctcggcaacc
     3001 tgcccggcaa ccactgcact cccacccacc tggcggaaac ggccgagtcc ctgggcaagc
     3061 agtacaagtt cgacgtcgag gtgctcgagc gcgacgacat ggagaagctc ggcatgggct
     3121 ccttcctgtc cgtggcgcgc ggctcgcacc agccgcccaa gttcattgtc atgcactaca
     3181 agggcggcaa ggccaaggcg aagccggtcg tgctcgtcgg caagggcatc accttcgaca
     3241 ccggcggcat ctcgctcaaa cccgcagccg agatggacga gatgaagttc gacatgtgcg
     3301 gcgccgcgag cgtgctcggc accttcaagg cggtcgcgca gatgggcctg ccgatcaacc
     3361 tggtcggcct cgtccccacg accgagaaca tgcccggcgg cggcgccacc aagcccggcg
     3421 acgtcgtcac ttcgatgtcg gggcagacca tcgaggtgct caacaccgac gccgagggcc
     3481 gcctgatcct gtgcgacgcg ctcacctacg ccg
//
LOCUS       DAOQBS010000208         3509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_29823_length_3509_cov_17.693399, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000208 DAOQBS010000000
VERSION     DAOQBS010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29823_length_3509_cov_17.693399"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKN25_14820"
     CDS             <1..201
                     /locus_tag="PKN25_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HNV92223.1"
                     /translation="MKRSRELGMQTFDQSLFQLYEDDLIGYEDALRNADSVNDLRLQI
                     KLNSRHGDPLAAGGGIQNLGIL"
     gene            complement(223..1386)
                     /locus_tag="PKN25_14825"
     CDS             complement(223..1386)
                     /locus_tag="PKN25_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNV92224.1"
                     /translation="MAETFFSPCPRGLEALLAEELVTFGARAARAVHGGVSWEGDWDA
                     CRRANLESRLATRVLWRVGQGRYRAEVDIYKLAYSVTWAKWFTADDTIRIYVTAQKSP
                     LKSLEFITLRVKDAVCDHFRTVAGRRPNVDTADPAVRIHLFLTVDTATLYVDTTGEPL
                     YKRGYKPAAVEAPLKENLAAGILRLTGWQPDEALVDPMCGSGTFLIEAAQMALDIAPG
                     LGRGFAFERLRIHDRGAWAALRRAAEQRRKPARVLPIFGSDVVGEWVRRSRMNLDAAG
                     LADCVRLDRADVLERTPPAAAGVMVANPPYGVRIGEAEELLAFYPRLGDALKQRWGGW
                     RCYLFSADPELPKRIGLKASRRTPLFNGALECRLFEYRMVAGSNRERTPRPQE"
     gene            complement(1563..2585)
                     /locus_tag="PKN25_14830"
     CDS             complement(1563..2585)
                     /locus_tag="PKN25_14830"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HNV92225.1"
                     /translation="MTTHAMRGQRGGWLAVLLLALMLWATALPLQAAAPQHGSTAADQ
                     AERQVERPLNNAPVWRAVRSGEEHFTLSRSPEAGVLIQSEGNTWRQLRNGPVTLYGGW
                     LLILVPSAILLFWLVKGTMKLHGQKTGRKMLRFSAFERFVHWGTAISFLLLAVTGVAI
                     LFGKHVLLPLIGHGGLGLLLNAGKLVHNYVGPLFGVFVVLMFFAFLRDNVWRPIDAQW
                     IRRAGGLLDGSHVPSGRFNFGEKTWFWFGVTFLGLTVAISGLIMDFPNLGDWSRADMQ
                     TANIVHAIGAILMLGLAFGHIYMGTLGAEGAYESMKTGYVDETWAREHHEQWYHDVKA
                     GKSGHP"
     gene            complement(2596..2832)
                     /locus_tag="PKN25_14835"
     CDS             complement(2596..2832)
                     /locus_tag="PKN25_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92226.1"
                     /translation="MSTGKLKSIALATLAGAALLGLSACSEVPQVTVYEQGQYRGKTD
                     ARPWEGGEFKGDRAAWEKALKERSRGQNEYNRIQ"
     gene            complement(2829..3467)
                     /gene="fdh3B"
                     /locus_tag="PKN25_14840"
     CDS             complement(2829..3467)
                     /gene="fdh3B"
                     /locus_tag="PKN25_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HNV92227.1"
                     /translation="MGRMKFLCDAERCIECNGCVTACKNEHEVPWGVNRRRVVTMNDG
                     VPGERSISVACMHCSDAPCMAVCPTDCFYKTEDGVVLHDKDKCIGCGYCFYACPFGAP
                     QFPNGPAAFGARGKMDKCTFCAGGPEETGSAAEYEKYGSNRLAQGKLPLCAEMCSTKA
                     LIAGDADVVANIFRERVLRRGTGAEVWGWDAAYGGGERKQERKQERKREGQS"
BASE COUNT          544 a         1243 c         1201 g          521 t
ORIGIN      
        1 atgaagcgct cgcgcgagct cggcatgcag accttcgacc agtcgctgtt ccagctctac
       61 gaggacgacc tgatcggcta cgaggacgcg ctgcgcaacg ccgactcggt gaacgacctg
      121 cgcctgcaga tcaagctcaa cagccgccac ggcgaccccc tcgcggccgg cggcggcatc
      181 cagaacctcg gcatcctgta gggcgggacg ccgcgcgcgt tgttactcct gcggacgcgg
      241 cgtgcgctcg cggttgctgc cggccaccat gcggtactcg aacaggcgac attcgagcgc
      301 gccgttgaag agcggcgtgc gccggctcgc cttcaagccg atgcgcttgg gcagctcggg
      361 gtcggcgctg aacagatagc agcgccagcc gccccagcgc tgcttgagcg cgtcgcccag
      421 gcggggatag aaggcgagga gttcctccgc ctcgccgata cgcaccccgt agggcggatt
      481 cgccaccatc acccccgcgg cggccggcgg cgtgcgctcg agaacgtcgg cgcggtccag
      541 gcgcacgcag tcggccagcc cggccgcgtc gaggttcatg cggctgcgcc gcacccactc
      601 gccgaccacg tccgaaccga agatcggcag cacgcgcgcc ggcttgcggc gctgctcggc
      661 ggcgcggcgc agcgccgccc aggcgccgcg gtcgtggatg cgcaggcgct cgaaggcgaa
      721 gccgcgcccg aggcccggcg cgatgtcgag cgccatctgc gcggcctcga tcaggaaggt
      781 gccgctgccg cacatcggat cgacgagggc ctcgtccggc tgccagcctg tgaggcgcag
      841 gatgccggcg gcgaggttct ccttcagcgg cgcctccacc gcagcaggct tgtagccacg
      901 cttgtagagc ggctcgccgg tggtgtcgac atacagcgtg gcggtgtcga cggtgaggaa
      961 caggtggatg cgcaccgccg ggtcggcggt atccacgttc ggccgccgtc cggccaccgt
     1021 gcggaagtgg tcgcacaccg catccttcac ccgcagcgtg atgaactcga ggctcttgag
     1081 cggcgacttc tgcgcggtga cgtagatgcg gatggtgtcg tcggcggtga accacttcgc
     1141 ccaggtgacg ctataggcga gcttgtagat gtcgacctcg gcgcgatagc gcccctgccc
     1201 cacccgccac agcacgcggg tggcgagccg gctctcgagg ttggcgcgcc ggcaggcgtc
     1261 ccagtcgccc tcccagctca ccccgccatg caccgcgcgc gccgcgcgcg cgccgaaagt
     1321 caccagctct tcggccagca gcgcctcgag gccgcgcggg cagggggaaa agaaggtttc
     1381 ggccatgtcg ggctccgggc ggacaaaaac ggattgtacc tagtgggccc atgcgccgca
     1441 gcgcgaaccc cgggaagccg acaaaacgcg gccccgcagg catgcgatgc cggtcggggc
     1501 cgaagcgtcc cgggccgaag ggatgggcgg cccggacgag ggagaggaga tggcgagccc
     1561 gctcagggat gcccggactt gcccgccttg acgtcgtggt accactgctc gtggtgctcg
     1621 cgcgcccagg tctcgtcgac gtagccggtc ttcatcgact cgtaggcgcc ttcggcgccg
     1681 agcgtgccca tgtagatgtg gccgaaggcc agccccagca tcaggatggc gccgatcgca
     1741 tgcacgatgt tcgcggtctg catgtcggca cggctccagt cacccaggtt cgggaagtcc
     1801 atgatcaggc cggagatcgc cacggtgagg ccgaggaagg tgacgccgaa ccagaaccag
     1861 gtcttctcgc cgaagttgaa gcgtcccgaa ggcacgtggc tgccgtcgag caggccgccg
     1921 gcgcggcgga tccactgcgc gtcgatcggc cgccagacgt tgtcgcgcag gaaggcgaag
     1981 aacatcagca ccacgaagac cccgaacagc ggaccgacgt agttgtgcac cagcttgccg
     2041 gcgttgagca gcaggcccag cccgccatgg ccgatgagcg gcagcagcac gtgcttgccg
     2101 aacaggatcg ccactccggt gacggcgagc agcaggaagc tgatcgccgt gccccagtgc
     2161 acgaagcgct cgaaagccga gaagcgcagc atcttgcgcc cggtcttctg cccgtgcagc
     2221 ttcatcgtgc ccttgaccag ccagaacagc aggatggccg aaggcacgag aatgagcagc
     2281 cagccgccgt agagcgtgac cgggccgttg cgaagctggc gccaggtgtt gccctcgctc
     2341 tggatcagca caccggcctc ggggctgcgc gagagggtga agtgctcctc gcccgagcgc
     2401 accgcgcgcc acaccggcgc gttgttgagc gggcgctcga cctggcgctc ggcctggtcc
     2461 gccgcggtac tgccgtgctg cggcgctgcc gcctgcagcg gcagcgccgt cgcccacagc
     2521 atgagcgcga gcagcagcac ggcgagccag ccgccgcgct gcccccgcat cgcgtgtgtc
     2581 gtcatgacga tctcctcact ggatgcggtt gtactcgttc tggccgcggg aacgctcctt
     2641 gagcgccttc tcccaggccg cgcggtcgcc cttgaactcg ccgccttccc aggggcgggc
     2701 gtcggtcttg ccgcggtact ggccctgctc gtacaccgtg acctgcggca cctcgctgca
     2761 ggccgacagg ccgagcagcg cagcgccggc cagcgtcgcg agcgcgatcg acttcagctt
     2821 gccggtactc atgactgccc ctcccgcttg cgttcctgct tgcgttcctg cttgcgctca
     2881 ccgccgccgt aggccgcatc ccagccccac acctcggcgc cggtgccgcg gcgcagcacg
     2941 cgctcgcgga agatgttggc caccacgtcg gcgtcgccgg cgatcagcgc cttggtcgag
     3001 cacatctcgg cgcacagcgg caacttgccc tgggcgaggc ggttggagcc gtacttctcg
     3061 tactcggcgg ccgagccggt ctcctcgggg ccgccggcgc agaaggtgca tttgtccatc
     3121 ttgccgcgcg caccgaaagc cgccgggccg ttggggaact gcggcgcgcc gaacggacag
     3181 gcatagaagc agtagccgca gccgatgcac ttgtccttgt cgtggagcac cacgccgtct
     3241 tcggtcttgt agaagcagtc ggtcgggcac accgccatgc agggcgcgtc cgagcagtgc
     3301 atgcaggcca ccgagatcga gcgctcgccc ggcacgccgt cgttcatcgt caccacccgg
     3361 cggcggttca cgccccaggg cacctcgtgc tcgttcttgc acgcggtgac gcagccgttg
     3421 cactcgatgc agcgctcggc gtcacagagg aatttcatgc gtcccatctt ttactctcct
     3481 cgtcctcagg ccttgctcac gcggcacag
//
LOCUS       DAOQBS010000209         3411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_31130_length_3411_cov_15.665673, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000209 DAOQBS010000000
VERSION     DAOQBS010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31130_length_3411_cov_15.665673"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PKN25_14845"
     CDS             complement(<1..335)
                     /locus_tag="PKN25_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV92228.1"
                     /translation="MSFTLRKLLAAALGTLTLAAGTAAVAADRLVIAATPVPHAEILE
                     FVKPMLAKEGVELEIKVFTDYVQPSMQTNEKRVDGNFFLHQPYLDAFKQKQKNDIQTV
                     VAKVHVEPLG"
     gene            complement(359..1018)
                     /locus_tag="PKN25_14850"
     CDS             complement(359..1018)
                     /locus_tag="PKN25_14850"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HNV92229.1"
                     /translation="MDLSVIDWSDIWIATWETLVMTGVSLLFTILLGLPLGILLFVTA
                     RRQLLEQGFVYTVLSFVVNVLRSVPFLILLIVMIPVTVILIGTSLGVEGAIPPLVVGT
                     APFFARLVENVLREVDRGVIEACQAMGIRTHRIIFGALLPEALPGLIAAVTVTAITLM
                     SYAAMSGVIGGGGLGDLAIRFGYQRFQTEVMVITVALLVVLVQIIQYSGDRLVLYFTR
                     K"
     gene            complement(1018..2046)
                     /locus_tag="PKN25_14855"
     CDS             complement(1018..2046)
                     /locus_tag="PKN25_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV92230.1"
                     /translation="MIRIENLRKTYRAADGREVEALADIELEIGAGEVFGIIGRSGAG
                     KSTLIRTLNLLERPSAGRVLIDGEDITTLDSEGLYALRRRVGMIFQHFNLLGAKTVAD
                     NIDWPLKATGHTNAAERAVRVKELLALVGLSEQGHKYPSQLSGGQKQRVGIARALANR
                     PQILLCDEATSALDPETTQSILRLLLDINRQLGLTIVLITHEMQVIRTICDRVAVIDG
                     GRIVESGRVADVFLHPRHPVTRSMVAQSDALAAASFDPAHAYMKDKLRGTLVRLTYIG
                     DVTYQPILSRIMAGAKVQITILQGEVSSIKDVPFGQLLLELEGSEDDIREVFAELDRH
                     QIHHEVLQ"
     gene            2195..3343
                     /locus_tag="PKN25_14860"
     CDS             2195..3343
                     /locus_tag="PKN25_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNV92231.1"
                     /translation="MQHDAKLSPATLAAQGTGATPMPHRDIVPPIHLATTFERAEDGS
                     LPGGRIYARDASPAYDPAEALLCELEGGARAALFASGMAAASAVLLALKPGARIVAPH
                     DMYWSLRNWMIGFAANWQIALDFYADAAELAALLQRPADLVWVETPANPTWEILDIAR
                     AAELAHAAGARLVVDSTVPTPVFTRPLALGADVVMHSATKYLNGHSDVVAGVLVTRAE
                     DDFWARIKTARAAGGAVLGPFEAWLLNRGLRTLFPRVRTAAASALRIAQHFEHHPALL
                     AVLYPGLPSHRGHDIACRQMEGGFGAMLSLRVRGGEDAARRVAGAVKIFRRATSLGSV
                     ESLIEHRGPIEGPGTRCPLDLLRLSVGIEDSDDLIADLEQALQQANEP"
BASE COUNT          528 a         1185 c         1175 g          523 t
ORIGIN      
        1 cccagcggct cgacgtggac cttggcgacc acggtctgga tgtcgttctt ctgcttctgc
       61 ttgaacgcgt cgaggtaggg ctggtgcagg aagaagttgc cgtcgacgcg cttctcgttg
      121 gtctgcatcg agggctggac gtagtcggtg aacaccttga tctcgagttc cacgccctcc
      181 ttggccagca tcggcttgac gaactcgagg atctcggcgt gcggcaccgg ggtggcggcg
      241 atgaccaggc ggtcggcggc cacggcggcg gtgccggcgg ccagggtgag cgtgccgagg
      301 gcggccgcca ggagtttgcg cagggtaaac gacatcgttg aagtctcctt cgtggggatc
      361 acttgcgggt gaaatacagc accaggcggt cgcccgagta ctggatgatc tggacgagca
      421 cgaccagcag ggcgacggtg atcaccatca cctcggtctg gaagcgctga tagccgaaac
      481 ggatcgcgag gtcgccgagg ccgccgccgc cgatcacgcc ggacatcgca gcataggaca
      541 tcaaggtgat cgccgtcacg gtgaccgcgg cgatcagccc gggcagggcc tcgggcagca
      601 gcgcgccgaa gatgatgcga tgcgtgcgga tgcccatcgc ctggcaggcc tcgatgacgc
      661 cgcggtcgac ctcgcgcagc acgttctcga ccaggcgcgc gaagaagggc gcggtgccca
      721 ccaccagcgg cgggatcgcg ccctcgaccc cgagcgaggt gccgatcagg atcaccgtga
      781 ccgggatcat cacgatcaag aggatcagga agggcaccga gcgcagcacg ttgaccacga
      841 aggacagcac cgtatagacg aagccctgct ccagcagctg gcgcctggcg gtgacgaaca
      901 gcaggatgcc cagcggcagg ccgagcagga tggtgaagag cagcgacacc ccggtcatga
      961 ccagggtctc ccaggtcgcg atccagatgt cgctccagtc gatgacggac aggtccatta
     1021 ctgcagcacc tcgtggtgga tctggtggcg gtcgagctcg gcgaacacct cgcggatgtc
     1081 gtcctcgctg ccctcgagct cgagcagcag ctggccgaag gggacgtcct tgatgctcga
     1141 gacctcgccc tgcaggatcg tgatctgcac cttcgcgccc gccatgatgc ggctcaggat
     1201 gggctggtag gtgacgtcgc cgatgtaggt caggcgcacc agcgtgccgc gcagcttgtc
     1261 cttcatgtag gcgtgcgccg gatcgaagct cgccgcggcg agcgcgtcgc tctgcgccac
     1321 catgctgcgc gtgaccggat gccgcgggtg caggaagacg tcggccaccc tgcccgactc
     1381 gacgatgcgc ccgccgtcga tcaccgcgac gcggtcgcag atggtgcgga tgacctgcat
     1441 ctcgtgggtg atcagcacga tcgtgaggcc gagctggcgg ttgatgtcga gcagcaggcg
     1501 caggatggac tgggtggtct cggggtcgag cgccgaggtc gcctcgtcgc acagcaggat
     1561 ctgcggccgg ttggcgagcg cgcgcgcgat gcccacgcgc tgcttctgcc cgcccgacag
     1621 ttgcgacgga tacttgtggc cctgctcgga cagtccgacc agcgcgagca gctccttcac
     1681 cctcaccgca cgctcggcgg cattcgtgtg cccggtcgcc ttcaggggcc agtcgatgtt
     1741 gtcggcgacg gtcttggcgc cgagcaggtt gaagtgctgg aaaatcatgc cgacgcgccg
     1801 acgcagcgcg tacaggccct cgctgtcgag cgtggtgatg tcctcgccgt cgatcagcac
     1861 gcgcccggcc gaggggcgtt cgagcaggtt gagggtacgg atcagcgtgc tcttgcccgc
     1921 gcccgagcgg ccgatgatgc cgaacacttc gccggcgccg atctcgagct cgatgtcggc
     1981 gagcgcctcc acctcgcggc cgtcggcggc acggtaggtc ttgcgcaggt tttcgatgcg
     2041 gatcacggga gctttccgga ctggaaaaaa gcctgagatc ttacccgcag cccggactcg
     2101 acaccactac ggggaacttg taggctcgcc gccacccggg cacccgccct cctgctcatc
     2161 ctcaccgcat cccccgcccg aaggaccgcg catcatgcag cacgacgcca agctctcccc
     2221 tgccaccctc gccgcccagg gcacgggcgc caccccgatg ccgcaccgcg acatcgtgcc
     2281 gccgatccac ctggcgacca ccttcgagcg cgccgaggac ggcagcctgc ccggcgggcg
     2341 catctacgcg cgcgacgcca gcccggccta cgaccccgcc gaggccctgc tgtgcgagct
     2401 ggaaggcggc gctcgagcgg cgctgttcgc ctccggcatg gccgcggcct cggcggtgct
     2461 gctggcgctg aagccgggcg cgcgcatcgt cgcgccgcac gacatgtact ggtcgctgcg
     2521 caactggatg atcggctttg ccgccaactg gcagatcgcg ctcgatttct acgccgacgc
     2581 cgcggagctc gccgcactgc tgcagcgccc ggccgacctg gtgtgggtgg agacgcccgc
     2641 aaacccgacc tgggaaatcc tcgacatcgc gcgcgccgcc gaactcgccc acgccgcggg
     2701 cgcgcgcctg gtggtggact ccaccgtgcc gaccccggtc ttcacccggc cgctggcgct
     2761 cggggccgac gtggtgatgc actcggcgac caaatatctg aacggccact cggacgtggt
     2821 cgccggcgtt ctggtgacgc gcgccgagga cgacttctgg gcgcgcatca agaccgcacg
     2881 ggccgccggc ggcgcggtgc tcggcccgtt cgaagcctgg ctgctcaacc gcggcctgcg
     2941 cacgctcttt ccccgcgtgc gcaccgctgc cgcctcggcg ctgcgcatcg cgcagcattt
     3001 cgaacatcac ccggcgctgc tcgccgtgct ctaccccggc ctgccctcgc accgcggtca
     3061 cgacatcgcc tgccgccaga tggaaggcgg cttcggcgcc atgctgtcgc tgcgcgtgag
     3121 aggcggcgag gatgccgcac gccgggtcgc cggcgcggtg aagatcttcc gccgcgcgac
     3181 ctcgctcggc tcggtcgaga gcctgatcga gcaccgcggc ccgatcgagg gtccgggcac
     3241 gcgttgcccg ctcgacctgc tgcgcctctc ggtgggcatc gaagatagcg acgacctgat
     3301 cgccgacctc gaacaggcgc tgcagcaagc aaacgagccc tgaagcccgg gcgggtttcg
     3361 tccgcaagcc gcccccacgt ttgcggtcgc ggggccgggg tgggggtcgc c
//
LOCUS       DAOQBS010000210         3383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_31487_length_3383_cov_14.391226, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000210 DAOQBS010000000
VERSION     DAOQBS010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31487_length_3383_cov_14.391226"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PKN25_14865"
     CDS             <1..998
                     /locus_tag="PKN25_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV92232.1"
                     /translation="MVYEYSQQAGGVITAIAAHFQSLARQRADYAAAEPLLSAAPAPR
                     PADGEDIAASGWRTLSLEGVCFAHGDGRASALELDRLALVRGRRYALVGPSGAGKTTL
                     LRLLAGLDAPTAGVLSIDGEAVPAAATAAVVGERVPSAGGLPSDAGGLAAMPARLRHL
                     ATLIPQQAEMFDGTLAENLLLGAGRTPERIEAALHAACADSVVEGLAQGLDTRVVEGG
                     ANWSGGQRQRLALARGVLAAAGTSLLLLDEPTSSLDPETEARVYQRLFAHFPDAALVS
                     SVHRLHLLDRFDEVILMRAGRIEAVGRAWELAQSSALFRELLAAQAVSGEAASQG"
     gene            complement(1158..1709)
                     /locus_tag="PKN25_14870"
     CDS             complement(1158..1709)
                     /locus_tag="PKN25_14870"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNV92233.1"
                     /translation="MGNRLSKIYTRTGDAGTTGLGDGKRVSKNSLRIHALGEVDETNA
                     IIGLLLCEELPEDVRVLLTNVQHDLFDLGGEVCIPGMSMITGKQVTHLEEELDRFNEP
                     LEPLKDFILPGGTRAAAYAHHARTVCRRAERALVALALEESVNDAPRQYLNRLSDLLF
                     VLGRVLNRAGGRGDVLWQKRKNA"
     gene            1832..2095
                     /locus_tag="PKN25_14875"
     CDS             1832..2095
                     /locus_tag="PKN25_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNV92234.1"
                     /translation="MTLSIRLDPELESELARAAAQTGRSKSELVKASLREYLARLAPR
                     KTPYELGKDLFGDPSATAATLDLTSKDRVRSTIVERLRAENDR"
     gene            2076..2492
                     /locus_tag="PKN25_14880"
     CDS             2076..2492
                     /locus_tag="PKN25_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV92235.1"
                     /translation="MLRTIADSGPLIALFEPAECNHVRVRRFIENYEGALLTTWPVLT
                     EVGHMLGHSVDRQLAFLQWVERGGVEVTTPGAGAVSLIRQLSEKYRDLPMDIADGSLI
                     VLALESGVRDILTLDRDFDVYRLPDRSRFNNVLATT"
     gene            2494..>3383
                     /locus_tag="PKN25_14885"
     CDS             2494..>3383
                     /locus_tag="PKN25_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNV92236.1"
                     /translation="MKSAEIRDAFLKFFESKGHQIVASSSLVPHEDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYTRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIAYAWELLTEVFKLPKDKLWVTVYAEDDEAYDIWTKEVGVPAERVIRIGDNKGAR
                     YASDNFWMMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNNVFMQFNRD
                     EQGVMHPLPKPSVDTGMGLERISAVLQHVHANYEIDLFQALIKAAARETCSADMDSPS
                     LKVLADHIRACSFLIADGVIP"
BASE COUNT          518 a         1176 c         1162 g          527 t
ORIGIN      
        1 tcatggtgta cgagtactcg cagcaggccg gcggggtgat caccgcgatc gcggcgcact
       61 tccagtcgct cgcgcgccag cgtgccgact acgccgcagc cgagccgctg ttgtccgccg
      121 cgccggcgcc gcgtccggcc gacggcgagg acatcgccgc ctcgggctgg cgcacgctga
      181 gcctggaggg cgtgtgcttc gcccatggcg acgggcgcgc gagcgcgctc gagctcgacc
      241 gcctggcgct cgtgcgcggc cgccgctatg ccctggtcgg cccgagcggc gcgggcaaga
      301 ccaccctgct gcgcctgctc gccgggctgg acgcgcccac ggcgggtgtc ctgtcgatcg
      361 acggcgaggc ggtgcccgca gccgccaccg cggcggtcgt cggcgagcgc gttcccagcg
      421 ctggcggctt gccgagcgac gccggggggc tggcggcgat gccggcgcgc ctgcgccatc
      481 tcgccaccct gatcccgcag caggccgaga tgttcgatgg cacgctggcc gagaacctgc
      541 tgctcggtgc cggtcgcacg cccgagcgca tcgaggccgc gctgcatgcg gcgtgcgcag
      601 actccgtggt cgagggcctg gcgcaggggc tggacacgcg ggtggtggag gggggcgcga
      661 actggtccgg cgggcagcgc cagcgcctcg cgctcgcccg cggcgtgctc gccgccgcag
      721 gcaccagcct gctgctgctc gacgaaccca ccagcagcct ggacccggag accgaggcgc
      781 gcgtctacca gcgcctgttc gcgcatttcc cggacgcggc gctggtgtcc tcggtgcacc
      841 gcctgcacct gctcgatcgt ttcgacgagg tcatcctgat gcgcgccggc cgcatcgagg
      901 cggtcggccg cgcctgggag ctggcgcaga gctcggcctt gttccgcgaa ctcctcgccg
      961 cccaggcggt gagcggagag gcggcgtcgc aggggtgaaa ccggggagcg gcacgcgctg
     1021 caccccgtcc gggcgcgccg gcatgagcga cgcccgtcgc gatcgggcgc ttccggggcg
     1081 gaggcgcgga ttccaagcgc cgcaatcgcg actcgcgtcg ctcctacgag aaacgacgtg
     1141 gctgcgggag gcggccctca ggcattcttc cgcttctgcc aaagcacgtc gccgcggccg
     1201 ccggcgcggt tgagcacgcg gccgagcacg aagaggaggt cggagaggcg gttgaggtac
     1261 tggcgcgggg cgtcattcac agactcctcg agtgccagcg ccaccagcgc gcgctcggcg
     1321 cggcggcaca ccgtgcgggc gtggtgggcg taggcggcgg cgcgggtgcc gccgggcagg
     1381 atgaagtcct tgagcggctc gagcggttcg ttgaagcggt cgagctcttc ttcaaggtgt
     1441 gtcacctgct tgccggtgat catgctcatg cccgggatgc acacctcgcc gccgaggtcg
     1501 aagaggtcgt gctgcacgtt ggtcagcagc acgcgcacgt cctcgggcag ctcctcgcac
     1561 agcagcaggc cgatgatggc gttggtctcg tcgacctcgc ccagggcgtg gatgcgcagg
     1621 ctgttcttgg agacgcgctt gccgtcgccc aggccggtgg tgccggcgtc gccggtgcgg
     1681 gtgtagatct tggagagccg attgcccatg ttgtcctcgt gtccggggtg gttgtcgtaa
     1741 cgcataatta taggcgtcac gggcggaaat tgacttcgat cgcagcgcgt catacgatgc
     1801 attacgttgt gtaatacagg aggccggccc catgaccctg tcgatccgtc tcgatcccga
     1861 gctcgagtcc gaactcgcgc gtgccgccgc gcagaccggc cgcagcaaga gcgagctggt
     1921 caaggccagc ctgcgcgaat acctcgcccg gcttgcgccg cgcaagacgc cctacgagct
     1981 cggcaaggac ctgttcggcg atccgtccgc cacggcggcg acgctcgatc tcaccagcaa
     2041 ggaccgcgtc cgtagcacca tcgtggagcg gctgcgtgct gagaacgatc gctgactccg
     2101 ggccgctgat cgcgctgttc gagcccgccg agtgcaacca cgtacgcgtg cgccgcttca
     2161 tcgagaacta cgagggggcg ctgctgacca cctggccggt gctgaccgaa gtcggtcata
     2221 tgctcggtca ttcggtcgat cgccagctcg ccttcctgca atgggtggag cgtggtggcg
     2281 tggaggtgac gacccccggc gcgggcgccg tctcgctgat ccgccagctc tccgaaaaat
     2341 accgcgacct gccgatggat atcgccgacg ggtcgctgat cgtgctcgcg ctcgaaagtg
     2401 gggtgcgcga catcctgaca ctcgaccggg acttcgacgt ctatcgtctc cccgatcgct
     2461 cccgattcaa caacgtcctg gccacgacct gacatgaaat ccgccgaaat ccgcgacgcc
     2521 ttcctcaagt tcttcgaatc caagggccac cagatcgtgg cctccagctc gctcgtgccg
     2581 cacgaggacc cgacgctgct gttcaccaac gccggcatga accagttcaa ggacgtgttc
     2641 ctcggcttcg acaagcgtcc ctacacccgc gccaccacct cgcagaagtg cgtgcgcgcc
     2701 ggcggcaagc acaacgacct cgagaacgtc ggctacaccg cgcgccacca caccttcttc
     2761 gagatgctcg gcaacttcag cttcggcgac tacttcaagc gcgacgccat cgcctacgcc
     2821 tgggagctgc tgaccgaggt cttcaagctg cccaaggaca agctctgggt cacggtgtac
     2881 gccgaggacg acgaggccta cgacatctgg accaaggagg tcggcgtgcc ggcggagcgc
     2941 gtgatccgca tcggcgacaa caagggcgcg cgctacgcct cggacaactt ctggatgatg
     3001 ggcgacaccg gcccttgcgg cccgtgcacc gagatcttct acgaccacgg cgaccacatc
     3061 tggggcggcc ctccgggatc gcccgaggaa gacggcgacc gctacatcga gatctggaac
     3121 aacgtgttca tgcagttcaa ccgcgacgag cagggcgtga tgcatccgct gcccaagccc
     3181 tcggtggaca ccggcatggg cctggagcgc atctcggcgg tgctgcagca cgtgcacgcc
     3241 aactacgaga tcgacctctt ccaggcgctg atcaaggcgg cggcgcggga aacctgcagt
     3301 gccgacatgg actcgccctc gctgaaggtg ctggccgacc acatccgcgc ctgctccttc
     3361 ctgatcgccg acggcgtgat ccc
//
LOCUS       DAOQBS010000211         3379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_31562_length_3379_cov_8.498195, whole genome shotgun sequence.
ACCESSION   DAOQBS010000211 DAOQBS010000000
VERSION     DAOQBS010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31562_length_3379_cov_8.498195"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKN25_14890"
     CDS             complement(<1..353)
                     /locus_tag="PKN25_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNV92237.1"
                     /translation="MRTPIQRGFGLVEAMVTIAVLAILLAVAVPSFQQMIVNGRTRTI
                     AESFRAGVDLARTEAMRRNLPVGFAVVSAVTGCSADTTGTNWVVFLGTTCNNDATVVQ
                     RFVAGGSEAGVTVSAS"
     gene            complement(350..778)
                     /locus_tag="PKN25_14895"
     CDS             complement(350..778)
                     /locus_tag="PKN25_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HNV92238.1"
                     /translation="MNHASRTRGFTLIELMIVVAIIGILASIALPAYSDYIRRSKIVE
                     ATSVLSDLRVRMEQFYQDNRSYVSSGTTCGVAMPTQQFFTMSCTGTSTTYTLTATGKS
                     GTDVAGFTYTLNQQNVRATTGGSFGTSTTCWVGKRGGGCL"
     gene            1003..1653
                     /locus_tag="PKN25_14900"
     CDS             1003..1653
                     /locus_tag="PKN25_14900"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HNV92239.1"
                     /translation="MDGWKAASVGVLAIAAGLGAGAAYAADVAVAGLFAGRAVLVVDG
                     GAPQTVKVGARTREGIVLRAATSEAATLEHDGRVFVARLGEQVASSAGTAASEISLQA
                     DGGGHFFTGARINGVAVNALVDTGATLVAMGRSDALRLGIDYRAGTMGQSATANGLAT
                     IWKVRLDAVELGGVRMLGVDAAVHETDLPVVLLGMSFLNRMDWRREGDRLLLKKRY"
     gene            1662..2285
                     /locus_tag="PKN25_14905"
     CDS             1662..2285
                     /locus_tag="PKN25_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNV92240.1"
                     /translation="MNPASGPHGLPPLEHIRRRLAAGPHRDGGFNGDGRDLARSRPPK
                     PAAVLVPLVVRDEVPSVLLTRRTDHLHHHPGEISFPGGRLEDDDPSPVHAALRETEEE
                     IGLARRHVELIGALPDYFTGTGFRVTPVVGVVHPPLELTLDAFEVAEAFEVPLAHFLD
                     PRNHQEHSILHEGRMRRFHAMPWHGYFIWGATAGILMSLYRLLNEEG"
     gene            2398..3321
                     /locus_tag="PKN25_14910"
     CDS             2398..3321
                     /locus_tag="PKN25_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HNV92241.1"
                     /translation="MTFFSLILALVLEQLRPLPARGWTVRVVRGVVGGMRGRFEGGSA
                     AQGRLAWLLLVPAAGFASALAFWLLWEMHPALAFLFNVVALYLCIGFRHDTRFFSAIH
                     MALRLGEPEGARSLLEDWRGTRHEGASASEIARLAIEEALVVSHRRLFGVMFWFVLLP
                     GPSGAVMYRLAGLLAEEWGRPDGGDAGFGGFALRAREAIEWLPARITAIVFSIMGNFE
                     DAIYCWRSQAVLWPERSSAILIASGGGALGVRLGMPVHVAGGIVDRPELGLGSEAGVD
                     HMQNTVSLLWRALFVCLFLLFLLAAAGWVGR"
BASE COUNT          453 a         1054 c         1290 g          582 t
ORIGIN      
        1 gaggcgctga ccgtgacgcc cgcctcacta cccccggcga cgaacctttg caccaccgtc
       61 gcgtcgttgt tgcaggtcgt gcccaagaag accacccagt tcgtgccggt ggtgtcggcg
      121 ctgcagccgg tgacggcgga gacgacggcg aacccgaccg gcagattgcg ccgcatcgcc
      181 tcggtacggg ccagatccac accggcgcgg aaggattcgg cgatggtgcg ggtgcggccg
      241 ttgacgatca tctgctggaa gctgggtacg gcgaccgcga gcaggatcgc taagactgcg
      301 atcgtcacca tggcctcgac caggccgaaa ccgcgctgga tcggcgtcct catagacagc
      361 cgcctcccct cttgccgacc cagcaggtcg tgctggtgcc gaagctgccg ccggttgtcg
      421 cgcggacgtt ttgctggttc agcgtgtacg tgaaccctgc cacgtccgtg ccggacttgc
      481 cggtcgcggt cagcgtgtag gtggtgctgg tgccggtgca actcatcgtg aagaactgct
      541 gtgtgggcat ggcgacgccg caggtggtgc cgctcgacac gtagctgcgg ttgtcctggt
      601 agaactgctc catgcgcacg cgcaggtcgg acagcaccga ggtcgcctcg acgatcttcg
      661 agcggcggat gtagtcggag taggcgggca gggcgatcga ggcgaggatg ccgatgatgg
      721 cgaccacgat catcagttcg atcagcgtga agccgcgggt gcgggaggcg tggttcaagg
      781 ggggctccgt gtcggatggg gcacagtctc gattgcattg tcggactttg ccgccggggt
      841 gttcgtgacg gattctatgg cgccacgcag aatgccgccg tcgcgggtgg acgaacggcg
      901 ggggctcgga ggatgatcgg cgggcggtgc gcagcgcggg caggcccgca gggcggatcg
      961 gcaacgggga gggaggtcgg cgcgatgcgg gggaaggacg ggatggacgg atggaaggcg
     1021 gcttcggtcg gcgtgctggc catcgcggcg ggcttgggcg cgggggcggc gtacgctgcc
     1081 gacgtggcgg tggccgggct gttcgcgggc cgggcggtgc tggtggtcga tggcggcgcg
     1141 ccgcagaccg tgaaggtggg cgcacggacg cgcgagggca tcgtgctgcg ggcggcgacg
     1201 agcgaggcgg ccacgctgga gcacgacggc cgtgtcttcg tcgcgcgcct gggcgagcag
     1261 gtggcgagca gcgcgggaac cgcggcgagc gagatcagtc tgcaggccga cgggggcggg
     1321 cacttcttta ccggcgcgcg catcaacggc gtggcggtca atgccctggt cgacaccggc
     1381 gccaccctgg tggcgatggg gcgcagcgat gcgctgcggc ttggcatcga ctaccgcgcc
     1441 ggcacgatgg gccagagcgc gaccgcgaac gggctggcga cgatctggaa ggtgaggctg
     1501 gacgcggtgg agctcggcgg cgtgcgcatg ctcggggtcg atgcggcggt gcacgagaca
     1561 gacctgcccg tggtgctgct cggcatgagt ttcctcaatc gaatggactg gcggcgcgag
     1621 ggcgaccgcc tgctgctcaa gaaacgttac tgaggcgtcg cgtgaatcct gctagcggac
     1681 cgcatggtct tccccctctg gaacacatcc gccgccggct ggccgcgggc ccgcaccggg
     1741 acggcgggtt caacggcgac ggccgcgacc tcgcgcgctc gcgcccgccc aagccggcgg
     1801 cggtgctggt gccgctcgtg gtgcgcgacg aggtgccgtc ggtgctgctc acccggcgca
     1861 ccgaccacct gcaccatcat ccgggcgaga tcagctttcc cggcggccgc ctcgaggacg
     1921 acgacccctc gccggtgcat gccgcgctgc gcgagacgga agaggagatc gggctcgccc
     1981 gccgtcacgt cgagctgatc ggcgcgctgc ccgactactt caccggtacc ggcttcaggg
     2041 tgaccccggt ggtcggcgtg gtgcatccgc cgctcgaact cacgctcgac gccttcgagg
     2101 tcgcggaggc cttcgaggtg ccgctcgcgc acttcctcga tccgcgcaac catcaggagc
     2161 acagcatcct gcacgagggg cggatgcgac gcttccacgc catgccctgg cacggctatt
     2221 tcatctgggg tgcgaccgcg gggatcctga tgtcgctgta tcgcctgctg aacgaggaag
     2281 gctgagcgcg gccaagggtt tccggcaggc ggcgcgcgct tgcctttgtg atatcttgcc
     2341 gcaccgatga ctcggctctg tgcccggggc cttttcggcc cgcgacgtcc tgcctgaatg
     2401 accttctttt ccttgatcct tgcgctcgtc ctcgaacagt tgcgtccgct gccggcgcgc
     2461 gggtggacgg tgcgcgtggt gcgcggcgtg gtcggcggaa tgcgagggcg cttcgagggc
     2521 ggctcggcgg cgcagggacg gctcgcgtgg ctcctgctgg ttcccgccgc cgggttcgcg
     2581 agcgcgctcg ccttctggct gctgtgggag atgcatcccg cgctggcctt cctgttcaac
     2641 gtggtggcgc tctacctctg catcggcttc cgccacgaca cccgcttctt ctcggccatc
     2701 cacatggcgc ttcgcctcgg cgagcccgag ggcgcgcgca gcctgctcga ggattggcgg
     2761 gggacccgcc acgagggggc ctccgcgagc gagatcgccc gcctcgcgat cgaggaggcg
     2821 ctggtcgtct cgcaccgccg gctgttcggc gtgatgttct ggttcgtgct gctacccggc
     2881 ccgagcggcg cggtgatgta ccggctcgcc ggactgctgg ccgaggagtg ggggcgcccg
     2941 gatgggggcg acgcgggctt cggcggcttc gcgctgcgcg cgcgcgaggc gatcgagtgg
     3001 ctgcctgcgc ggatcaccgc gatcgttttt tccatcatgg gcaatttcga ggatgcgatc
     3061 tactgctggc gctcgcaggc ggtgctgtgg ccggagaggt cgtcggctat acttatcgct
     3121 agtggtggtg gcgcgctggg cgtgcggctc ggcatgccgg tccatgtggc cgggggcatc
     3181 gtggatcggc ccgaactggg gctggggagc gaggcaggcg tcgatcacat gcaaaacacg
     3241 gtgagcctgc tatggcgggc tctgttcgtg tgtctgttcc tgctcttcct gctcgcggct
     3301 gcgggctggg tggggcggtg acaagtctaa tctcaacaag ggagtgggtt atggcaaatc
     3361 gtgaagtcgt cgttctgag
//
LOCUS       DAOQBS010000212         3342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_32093_length_3342_cov_14.961059, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000212 DAOQBS010000000
VERSION     DAOQBS010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32093_length_3342_cov_14.961059"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PKN25_14915"
     CDS             complement(<1..739)
                     /locus_tag="PKN25_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNV92242.1"
                     /translation="MNAVADTQFTDFVVADLALAAWGRKEIRIAETEMPGLMAIREEY
                     AASQPLKGARITGSLHMTIQTAVLIETLTALGAQVRWASCNIFSTQDHAAAAIAADGI
                     PVFAVKGESLKDYWDYTHRIFEWSDGGYSNMILDDGGDATLLLHLGARAEQDLSVLAK
                     PGSEEETILFAAIKAKLATAPTWYSTRLAHIKGVTEETTTGVHRLYQMHARGELRFPA
                     INVNDSVTKSKFDNLYGCRESLVDGIKR"
     regulatory      complement(842..946)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(1139..2212)
                     /locus_tag="PKN25_14920"
     CDS             complement(1139..2212)
                     /locus_tag="PKN25_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNV92243.1"
                     /translation="MLQQVLMFALGLTVLVVGADVLVRGASRLAVSFGVSPLVVGLTV
                     VAFGTSAPEMAVSVGSALAGSPDLALGNVVGSNICNVLLILGISALITPLLVDEQIIR
                     QEIPIMIGVSALLVVMALDGHLGLVESIVLFALVIAYTVFLIVQSRRASKAVQEEFGT
                     DLPTSAWDRHWAVQVGLIATGLVMLIVGADWLVEAAVAFARAFGVSDLVVGLTVVAVG
                     TSMPEIATSIVAAMRGQRDIAVGNVVGSNIFNILAVLGASGIASGTGLPVSEAARNFD
                     LWVMLAVAFACLPIMITGREIARWEGGVFLAYYAAYTAWLVLQAQQHTSLPAFSGIML
                     GYVMPLTVITVVVSIVRSNGRRA"
     gene            complement(2519..>3342)
                     /gene="metK"
                     /locus_tag="PKN25_14925"
     CDS             complement(2519..>3342)
                     /gene="metK"
                     /locus_tag="PKN25_14925"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNV92244.1"
                     /translation="LDQGAGDQGLMFGYATNETASLMPLPIHYAHRVMQRQAEVRKDG
                     RLPWLRPDAKSQLTVKYVDGKPVAIDTVVVSTQHDPDVTQEQIREAVIELIIKPVLPP
                     ELMQGDVRYLVNPTGRFVVGGPHGDCGLTGRKIIVDTYGGAAHHGGGAFSGKDPSKVD
                     RSAAYAGRYVAKNVVAAGLADRCEVQVAYAIGVARPVSLMVNTFGTGRIADEKIVDLI
                     GRHFDLRPKAIIQTLDLLRPIYSRTAAYGHFGRDEPGFTWEQTDKAAALRADAGL"
BASE COUNT          526 a         1108 c         1218 g          490 t
ORIGIN      
        1 cgcgcttgat gccgtcgacc agcgattcgc ggcagccgta gaggttgtcg aacttcgact
       61 tggtgaccga atcgttgacg ttgatcgccg ggaacctgag ctcgccgcgc gcgtgcatct
      121 ggtacaggcg atgcacgccg gtggtggtct cctcggtcac gcccttgatg tgcgccagcc
      181 gcgtcgagta ccaggtgggg gcggtagcga gcttggcctt gatcgcggca aagaggatgg
      241 tctcctcttc cgagcccggc ttggccagca ccgagaggtc ctgctccgcg cgcgcgccca
      301 ggtgcagcag cagcgtggcg tcgccgccgt cgtcgaggat catgttgctg taacccccgt
      361 ccgaccattc gaagatgcgg tgggtatagt cccagtagtc cttcagcgac tcgcccttga
      421 ccgcgaacac cgggatgccg tcggcggcga tggccgcggc ggcgtggtcc tgggtcgaga
      481 agatgttgca cgaggcccag cgcacctggg cgccgagcgc ggtcagcgtc tcgatcagca
      541 ccgcggtctg gatggtcatg tgcagcgagc cggtgatgcg tgcgcccttc agcggctggc
      601 tggcggcgta ttcctcgcgg atcgccatca ggccgggcat ctcggtctcg gcgatgcgga
      661 tttccttgcg gccccaggcg gcgagcgcga ggtcggcgac gacgaaatcg gtgaattggg
      721 tgtcagccac ggcgttcatg tgaagctcca tgctatggcc gggcggaaag cggggggagg
      781 gcagcatgtc gctgcaggcg gctcacccga catcccgaac ccggcagggt tgaagacgat
      841 tcgcaggtga gcgccgttgt actgataccc gccgcagggc ggggtccgag cctgggggcc
      901 tggcagcagc ggatgcggcc ggcgcctcgc agcgctcctc ggaaagaagc gaatgatacc
      961 aaagcggcag gcgggcgtgg actcgagggg tcggttgctg ccgcgggcat cacgatcgcg
     1021 gggatcgcga ctggcgtcgc tcccacatcc ggccccggtc gccgccggct cccgcaggag
     1081 cgacgccagt cgcgatcagg cggcccggca tgcgaggcac gcagcccgat cgccccgcct
     1141 atgcgcgcct gccgttgctg cgcacgatgc tgaccacgac ggtgatcacc gtgagcggca
     1201 tcacgtagcc cagcatgatc cccgagaagg ccggcaggct ggtgtgctgc tgggcctgca
     1261 gcaccagcca ggcggtgtag gcggcgtagt aggcgaggaa cacgccgccc tcccagcgcg
     1321 cgatctcgcg cccggtgatc atgatcggca ggcaggcgaa ggccaccgcg agcatcaccc
     1381 agaggtcgaa gttgcgcgcc gcctccgaca ccggcaggcc ggtgcccgag gcgatgcccg
     1441 aggcaccgag cacggcgagg atgttgaaga tgttgctgcc gaccacgttg cccaccgcga
     1501 tgtcgcgctg gccgcgcatc gcggcgacga tggaggtggc gatctcgggc atcgaggtgc
     1561 ccaccgcgac cacggtgagg cccaccacca ggtcgctgac cccgaaggcg cgcgcgaagg
     1621 ccaccgccgc ttccaccaac cagtcggcgc cgacgatcag catcaccagt ccggtcgcga
     1681 tcagcccgac ctgcaccgcc cagtggcggt cccaggcgga ggtggggagg tcggtgccga
     1741 actcctcctg caccgccttg gaggcgcgcc gcgactgcac gatcaggaac accgtgtagg
     1801 cgatcaccag ggcgaacagc acgatcgact cgaccaggcc cagatggccg tcgagcgcca
     1861 tcaccaccag cagggccgac acgccgatca tgatcgggat ctcctggcgg atgatctgct
     1921 cgtccaccag cagcggggtg atcagcgccg agatgccgag gatcagcagc acgttgcaga
     1981 tgttgctgcc gaccacgttg ccgagcgcga ggtcggggct gccggcgagc gccgagccca
     2041 ccgataccgc catctccggc gcgctggtgc cgaaggcgac cacggtgagg ccgacgacga
     2101 gcggcgaaac gccgaaggag accgccagcc gcgaggcgcc acgtacgagg acgtccgcac
     2161 cgacaacgag gacggtgagg ccgagggcga acatcagtac ttgttggagc atgggcgact
     2221 ctgggcgacg tggtggggca gagaccgggg cgggcgtccg cgagttccgg attctaccgg
     2281 gacggatgcg cggcggttct gcgcaggagc gacgcaagtc gcgattgcag cgctgggaac
     2341 ccgcgccagc gccgcagcgc cgcccgagcg cgactcgcgg cgttcccgcg cgcgcattcc
     2401 cgagcgtggc tgacctgcgt aggagcgacg caagtcgcga ttgcagcgct cggaacccgc
     2461 gccagcgccg cagcgccgcc cgatcgcgac tcgcgtcgtt cctgagggcg cttccggctc
     2521 agagtccggc gtcggcgcgc agggcggcgg ccttgtcggt ctgttcccag gtgaagcccg
     2581 gctcgtcgcg gccgaagtgg ccgtaggcgg cggtgcggga atagatcggg cgcagcaggt
     2641 cgagcgtctg gatgatcgcc ttcgggcgca ggtcgaaatg ccggccgatg agatcgacga
     2701 tcttctcgtc ggcgatcctg ccggtgccga aggtgttgac catcagcgaa accgggcgcg
     2761 ccacaccgat cgcgtaggcg acctggacct cgcaccggtc ggcgaggccg gcggcgacca
     2821 cgttcttggc cacgtagcga ccggcgtagg cggccgagcg gtcgaccttg gacggatcct
     2881 tgcccgagaa cgcaccgccg ccgtggtggg ccgcgccgcc gtaggtgtcg acgatgatct
     2941 tgcgcccggt gaggccgcag tcgccgtgcg ggccgccgac cacgaagcgg ccggtggggt
     3001 tcaccaggta gcgcacgtcg ccctgcatca gctcgggcgg cagcaccggc ttgatgatca
     3061 gctcgatcac cgcctcgcgg atctgctcct gggtcacgtc ggggtcgtgc tgggtggaca
     3121 ccacgacggt gtcgatcgcc accggcttgc cgtcgacgta cttgacggtg agctggctct
     3181 tggcgtccgg gcgcagccag ggcaggcggc catccttgcg cacctcggcc tggcgctgca
     3241 tgacgcggtg agcgtagtgg atcggcagcg gcatcaggct ggcggtctcg ttggtggcgt
     3301 agccgaacat caggccctgg tcgccggcgc cctggtcgag cg
//
LOCUS       DAOQBS010000213         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_32490_length_3315_cov_13.762270, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000213 DAOQBS010000000
VERSION     DAOQBS010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32490_length_3315_cov_13.762270"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..772
                     /locus_tag="PKN25_14930"
     CDS             8..772
                     /locus_tag="PKN25_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNV92245.1"
                     /translation="MTSVAVECADGVGHLELNRADALNALDLQFCKEIDDALLALDAR
                     EDVRAILISTSLKHFCAGADIREMLDMSAEQARATGFVGCVSAPPRLRKPLIVAVRGL
                     AAGGGCELVEMCDIVVAAASARFCHPEITLAAMPGAGGTQRLARVVGKHVAMDLLLTG
                     RALSADEALVAGLVSRVVPDEALEAAAMSVARLVASYSSPVACRIKAAVNGAQVGLDA
                     GLALERELFHACFSEHDFREGLAAFTEKRKPRFEHR"
     gene            903..2327
                     /gene="yegQ"
                     /locus_tag="PKN25_14935"
     CDS             903..2327
                     /gene="yegQ"
                     /locus_tag="PKN25_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HNV92246.1"
                     /translation="MPASPPAPRRPELLAPAGTLDMMRTAFAYGADAVYAGQPRYSLR
                     VRNNHFGQLDTLAAGMAEARAAGKLFYLVANIYPHNAKLRTFEDDMAPVIALQPDALI
                     MADPGLILMVRERWPEVPIHLSVQANTTNYASVRFWQSVGVKRIILSRELSLDEVAEI
                     RDACPDMELEVFVHGALCIAYSGRCLLSGYFNHRDPNQGSCTNSCRWDYKLHEAAEDA
                     AGDIQACGATPIGNPKDAGAVGTATRSALDTAQGLALGGGPRHLGGSKVWLLEEGTRP
                     GERMPIEEDEHGTYILNSKDLRAIEHVQRLVEIGVDSLKIEGRTKSPYYVARAAQGYR
                     RAIDDAVAGRPFDVRLLGELEGLASRGYTDGFYQRHSTPEQQNYLRGHSESGRSLLVG
                     EVVGWDAARGLAEVEVKNGFGVGDRLEFVQPGGNTEAVLERLFGADGEAIQRVPGSGR
                     RVWLALPADADADPARPCFIARFL"
     gene            2488..3156
                     /locus_tag="PKN25_14940"
     CDS             2488..3156
                     /locus_tag="PKN25_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNV92247.1"
                     /translation="MILNIDLVSDFVCPWCFLGAVRLDRALAELRATRPDIEPRINWL
                     PFFLNPDTPPAGEPYRAFLEGKFGGPVKAEAVLDQVREAAAGDGLQYAFERIATRPNT
                     LAAHRLLYRAQAHGHKPERVRALAHALFAAHFQEGRDIGDLDTLADLAAACGDRRELV
                     REWLERGTDADKVQRMAAGLRRQGIEGVPFFILDRRLSLSGAQSAAVIGAALLQALET
                     GKPS"
BASE COUNT          480 a         1219 c         1133 g          483 t
ORIGIN      
        1 aacgacaatg acctcggttg ccgtcgaatg cgctgatggc gtcggccatc ttgagctgaa
       61 tcgcgccgat gcgctgaatg cgctggacct gcagttctgc aaggaaatcg acgatgcgct
      121 cctggccttg gacgcgcgcg aagacgtgcg ggcgatcctc atctccacgt cgctgaagca
      181 cttctgtgcc ggggcggaca tccgggagat gctggacatg agcgccgaac aggccagggc
      241 gacggggttt gttggctgcg tttccgcgcc accccggctc cggaagccct tgatcgttgc
      301 cgtgagggga ctggcggcgg gtggcgggtg cgagctggtg gagatgtgcg acatcgtcgt
      361 cgccgccgct tcggcgcgct tctgccaccc cgagatcacg ctggccgcca tgccgggcgc
      421 gggcggtacg cagcggctgg cgcgggtcgt gggcaagcat gtcgcgatgg atctgctgct
      481 caccgggcgc gcgctgagcg cggacgaggc gcttgtcgcc ggcctggtgt cgcgcgtggt
      541 tccggatgag gcgctcgaag ccgctgccat gtccgtcgcc cggctggtgg cgtcgtactc
      601 gagcccggtc gcctgcagga tcaaggccgc ggtcaatggg gcgcaggtgg ggctcgatgc
      661 cggccttgcc ctggagcgcg agctgttcca cgcgtgtttc tccgagcatg actttcgcga
      721 ggggcttgcc gcgttcacgg aaaagcggaa gccgagattc gagcatcgtt gagggcttga
      781 atgccggccg ccgcagccgg gtgacgtggc ggaggtgcgg cagatcgatc atcgcagcaa
      841 gaatccaccg ccggcgcccc ccgcccgcgc aggtaaaatg cgcttttgcc cgcccgcccc
      901 cgatgcccgc ctcccccccg gccccgcgcc gccccgaact gctcgccccc gccggcaccc
      961 tcgacatgat gcgcaccgcc ttcgcctatg gcgcggacgc ggtctacgcc ggccagccgc
     1021 gctattcgct gcgcgtgcgc aacaaccact tcggccagct cgacacgctc gccgccggca
     1081 tggccgaggc ccgcgccgcc ggcaagctgt tctatctcgt cgccaacatc tatccgcaca
     1141 acgccaagct gcgcaccttc gaggacgaca tggcgccggt gatcgcgctg cagcccgacg
     1201 cgctgatcat ggccgacccc gggctgatcc tgatggtgcg cgagcgctgg cccgaggtgc
     1261 cgatccacct ctcggtgcag gccaacacca ccaactacgc ttcggtgcgc ttctggcagt
     1321 cggtgggggt caagcgcatc atcctgtcgc gcgagctgtc gctggacgag gtcgccgaga
     1381 tccgcgacgc ctgcccggac atggagctgg aagtcttcgt gcacggtgcg ctgtgcatcg
     1441 cctactccgg gcgctgcctg ctgtcgggct acttcaacca ccgcgacccc aaccagggca
     1501 gctgcaccaa ctcctgccgc tgggactaca agctgcacga ggccgccgag gacgccgccg
     1561 gcgacatcca ggcctgcggc gccacgccga tcggcaaccc caaggacgcc ggcgcggtcg
     1621 gcaccgccac ccgcagcgcg ctcgacaccg cgcaggggct ggcgctcggc ggcggtccgc
     1681 gccacctcgg cggcagcaag gtgtggctgc tggaggaggg cacccgcccg ggcgagcgga
     1741 tgccgatcga ggaagacgag cacggcacct acatcctcaa ctcgaaggat ctgcgcgcga
     1801 tcgagcacgt gcagcgcctg gtcgagatcg gcgtcgattc gctcaagatc gagggccgca
     1861 ccaagagccc ctactacgtc gcccgcgccg cccagggcta ccggcgcgcg atcgacgatg
     1921 cggtggccgg gcggcccttc gatgtacgcc tgctcggcga actcgagggc ctggccagcc
     1981 gcggctacac cgacggcttc tatcagcgcc actccacccc cgagcagcag aactacctgc
     2041 gcggccactc cgaatccggg cgcagcctgc tggtgggcga ggtggtcggc tgggatgccg
     2101 cgcgcggcct ggccgaggtc gaggtcaaga acggcttcgg cgtcggcgac cggctggagt
     2161 tcgtacagcc gggcggcaac accgaggcgg tgctggagcg gctgttcggc gccgatggcg
     2221 aggcgatcca gcgcgtgccg ggcagcggcc ggcgcgtctg gctggcgctg ccggcggatg
     2281 cggatgcgga cccggcgcga ccctgcttca tcgcgcgctt tctgtgaagc gcgaccaccg
     2341 ccggccgggc gcggtcgtgt gtcgccatca aggcgggctt caacgtggac gcgcggtgtt
     2401 caggaatcct ttaacctgag ccccgccgcg gacgacgtgc ccgcatcccc gcttccccag
     2461 ccgtacatcg aaactcaccc cctccccatg atcctgaaca tcgacctcgt ttccgacttc
     2521 gtctgcccgt ggtgcttcct cggcgccgtc cgcctcgacc gcgcgctcgc cgagctgcgc
     2581 gcgacgcgcc ccgacatcga gccgcgcatc aactggctgc ccttcttcct caacccggac
     2641 actccgcccg cgggcgagcc ctaccgcgcc ttcctggagg gcaagttcgg cggtccggtg
     2701 aaggccgagg cggtgctgga ccaggtgcgc gaggctgccg ccggcgacgg gctgcagtac
     2761 gccttcgagc gcatcgccac gcgcccgaac acgctcgccg cgcaccgcct gctctaccgt
     2821 gcccaggccc acggccacaa gccggagcgc gtgcgcgcgc tcgcccacgc gctgttcgcc
     2881 gcgcacttcc aggaggggcg cgacatcggc gacctcgaca ccctggccga tctcgccgcc
     2941 gcctgcggcg accgccgcga actggtgcgc gagtggctgg agcgcggcac cgatgccgac
     3001 aaggtgcagc gcatggccgc cggactgcgt cgccagggca tcgagggcgt gcccttcttc
     3061 atcctcgacc gccgcctcag cctgtcgggc gcacagtccg ccgcggtgat cggcgccgcg
     3121 ctgctgcagg cgctggaaac cggcaaaccg tcctgagggc gggaacgccc gccgccaccg
     3181 acgcgtctga gcagggacac cctcaccgga cccgatcgcg atgaacgcaa gccacgaaac
     3241 ccgccacatg ctcgacgccg ccgacctgcg cgcgctcgcc gaggcgcgcg tgctgctcga
     3301 gcggcccagc ctggc
//
LOCUS       DAOQBS010000214         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_32778_length_3296_cov_10.883678, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000214 DAOQBS010000000
VERSION     DAOQBS010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32778_length_3296_cov_10.883678"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PKN25_14945"
     CDS             complement(<1..658)
                     /locus_tag="PKN25_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV92248.1"
                     /translation="MSEKAASDVYLSSNSPILIKISGQILQLSDQPLTHAQPRQLLAE
                     LLTPSQLEELDDTGELNVGIGLAGVGSFRLSAFKQRGSIAAVFRCIPFQIPTLESLNV
                     PPILSNLILEKRGLILMVGATGTGKSTTLASMLEQRNQQLAGHILTIEDPIEFLFSNK
                     KSVVNQREVGRDTQSLQVGLRNALRQAPDCILIGEIRDRETMTAAISYALSGHLVLST
                     L"
     gene            complement(753..1574)
                     /gene="dapD"
                     /locus_tag="PKN25_14950"
     CDS             complement(753..1574)
                     /gene="dapD"
                     /locus_tag="PKN25_14950"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNV92249.1"
                     /translation="MTQHLQNLIDAAWENRAQVSAAATPEVRDAVEHVIAEMDAGRIR
                     VAERQGVGQWTVNQWVKKAVLLSFRLRDNQVMRSGDIAYYDKVPTKFAQFDEADFAAM
                     GARVVPGAIARRGSFIAKNAILMPSFVNIGGYVDEGTMVDTWASVGSCAQIGKNVHLS
                     GGVGIGGVLEPMQANPTIIEDNCFIGARSEIVEGVIVEENSVISMGVYIGQSTRIYDR
                     ATGESSYGRIPAGSVVVSGNLPSADGKYSLYCAVIVKRVDAQTRAKTSINDLLRA"
     gene            complement(1571..2782)
                     /gene="dapC"
                     /locus_tag="PKN25_14955"
     CDS             complement(1571..2782)
                     /gene="dapC"
                     /locus_tag="PKN25_14955"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HNV92250.1"
                     /translation="MNPLLARLHPYPFERLRALTREITPNPTYKPIGLGIGEPKHATP
                     ELIKRALSDNLQGLASYPATAGEPALREACAAWVQRRYGVTLDAASQVLPVNGSREAL
                     FALAQTVIDPTKPRATVVCPNPFYQIYEGAALLAGAQTAFVNSDPARNFASDWASVPE
                     AVWARTQLLYVCSPGNPTGAVMPLAEWKMLFELSDRHGFVIASDECYSEIYFRDEPPL
                     GALQAAQALGRDRRNLVVLTSLSKRSNVPGMRSGFVAGDAAILKQFLLYRTYHGSAMS
                     PVVQHASVAAWNDEAHVVDNRDKYRAKFAQVTPVLAQVLDVRLPDAGFYLWADVTPAR
                     PDGDDVAFALGLLAQYNVAVLPGSLLARRAQGLNPGAGRIRLALVAEVDECLEAAHRI
                     VSFVQSQRSQP"
     gene            complement(2804..>3296)
                     /gene="queF"
                     /locus_tag="PKN25_14960"
     CDS             complement(2804..>3296)
                     /gene="queF"
                     /locus_tag="PKN25_14960"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNV92251.1"
                     /translation="TATTRPSGGKSRAARRLNERPLPPNPPSAPSKDLHVFPNPAPGR
                     DYVIQFQVPEFTCHCPLTGQPDFAHFTIDMIADELCVELKSLKMYFWSYRHEGAFHEK
                     VTNTILDDIVKATKPRFIRITAKWYVRGGIYTNVVAEHRKKGWKPKPLVELPQHAAQT
                     GIL"
BASE COUNT          462 a         1084 c         1189 g          561 t
ORIGIN      
        1 gcagcgtcga cagcaccagg tggcccgaca gcgcgtacga gatcgccgcg gtcatggtct
       61 cgcggtcgcg gatctcgccg atcaggatgc agtcgggtgc ctggcgcagc gcgttcctca
      121 gcccgacctg cagcgactgc gtgtcgcgcc cgacctcgcg ctggttgacc accgacttct
      181 tgttgctgaa caggaactcg atcgggtcct cgatcgtcag gatgtgcccg gcgagctgct
      241 ggttgcgctg ctcgagcatc gacgcgagcg tggtgctctt gccggtgccg gtggcgccga
      301 ccatcaggat caggccgcgc ttctcgagga tcaggttgct caggatcggc gggacgttca
      361 ggctctcgag cgtcgggatc tggaacggga tgcagcggaa caccgcggcg atcgagccgc
      421 gctgcttgaa cgcgctcagg cggaagctgc ccacgccggc caggccgatg ccgacgttga
      481 gctcgccggt gtcgtcgagt tcctcgagct ggctcggcgt cagcagctcg gcgagcagct
      541 ggcgcggctg ggcgtgcgtc agcggctggt cgctgagctg caggatctgg ccgctgatct
      601 tgatgaggat cggcgagttc gacgacaggt agacgtcgga cgccgctttc tcggacatca
      661 acctcagcac ccgctccatg ttcccaccgc tcatgccgac cccttggttg tgtgtggacg
      721 atcgcgccgc caggccgcgc cgcggcgccg gctcaagcgc gcagcaggtc gttgatgctg
      781 gtcttggcgc gcgtctgggc gtcgacacgc ttgacgatca ccgcacagta caggctgtac
      841 ttgccgtcgg cgctgggcag gttgccgctg acgaccaccg agcccgccgg gatgcggccg
      901 tagctcgact cgccggtggc gcggtcgtag atgcgcgtgc tctggccgat gtagacgccc
      961 atgctgatga ccgagttctc ctcgacgatc acgccttcga cgatctcgga gcgcgcgccg
     1021 atgaagcagt tgtcctcgat gatggtcggg ttggcctgca tgggctcgag cacgccgccg
     1081 atgccgacgc cgcccgacag gtgcacgttc ttgccgatct gcgcgcacga gcccaccgac
     1141 gcccaggtgt cgaccatcgt gccctcgtcg acgtagccgc cgatgttgac gaacgagggc
     1201 atcaggatcg cgttcttcgc gatgaagctg ccgcggcggg cgatggcgcc gggcaccacg
     1261 cgcgcgccca tcgcggcgaa gtccgcctcg tcgaactgcg cgaacttggt cggcaccttg
     1321 tcgtagtagg cgatgtcgcc ggagcgcatg acctggttgt cgcgcaggcg gaagctcagc
     1381 agcaccgcct tcttgaccca ctggttcacg gtccactggc ccacgccctg gcgctcggcg
     1441 acgcggatgc ggccggcgtc catttcggcg atcacgtgct cgaccgcgtc gcgcacctcg
     1501 ggcgtggcgg cggcggagac ctgcgcgcgg ttttcccagg cggcatcgat gaggttctgg
     1561 aggtgttggg tcatggctgg cttctctggg attgcacgaa cgaaacgatg cggtgcgcgg
     1621 cctcgaggca ttcgtcgacc tcggccacca gcgcgagccg gatgcggccc gcgcccgggt
     1681 tcaggccctg cgcgcggcgc gcgagcaggc tgcccggcag caccgcgaca ttgtattgag
     1741 ccagcaggcc cagggcgaac gcgacgtcgt cgccgtcggg gcgcgccggc gtgacatccg
     1801 cccacagata gaagccggcg tcgggcaggc gcacgtcgag cacctgcgcc agcaccggcg
     1861 tgacctgggc gaacttggcg cggtacttgt cgcggttgtc gaccacgtgc gcctcgtcgt
     1921 tccaagcggc cacgctggcg tgctgcacca ccgggctcat cgcgctgccg tggtaggtgc
     1981 ggtagagcag gaactgcttc aggatcgccg cgtcgcccgc gacgaagccg ctgcgcatgc
     2041 cgggcacgtt ggagcgcttc gacaggctcg tcagcacgac caggttgcgc cggtcgcggc
     2101 cgagcgcctg ggcggcctgc aacgcgccca gcggcggctc gtcgcggaag tagatctcgg
     2161 agtagcactc gtcggaggcg atcacgaagc cgtggcggtc gctcagctcg aagagcatct
     2221 tccattcggc cagcggcatc acggcgccgg tcgggttgcc cggcgagcac acgtacagca
     2281 gctgcgtgcg cgcccacacg gcctcgggca cgctggccca gtcggaggcg aagttgcgcg
     2341 ccgggtcgct gttgacgaag gccgtctgcg ccccggcgag cagggccgcg ccctcataga
     2401 tctgatagaa cggattcggg cagaccacgg tggcgcgcgg cttcgtcggg tcgatcaccg
     2461 tctgcgccag agcgaacagc gcctcgcgcg agccgttcac cggcagcacc tgcgaggccg
     2521 cgtcgagggt gacgccgtag cggcgctgca cccaggccgc gcaggcctcg cgcagtgccg
     2581 gttcgccggc cgtggccggg tagctggcca ggccttgcag gttgtcgctc agcgcgcgct
     2641 tgatgagttc cggcgtcgca tgcttgggct cgccgatgcc caggccgatc ggcttgtagg
     2701 tcggattcgg ggtgatctcg cgcgtcagcg cacgcagtcg ttcgaacggg taggggtgca
     2761 ggcgcgccag cagcggattc atcggcggat tatcgggtgc ggcctacagg atgccggtct
     2821 gcgccgcatg ctgcggcagc tcgacgagcg gcttcggctt ccagcccttc ttgcggtgct
     2881 cggccaccac gttggtgtag atgccgccgc gcacgtacca cttggcggtg atgcggatga
     2941 agcgcggctt cgtcgccttg acgatgtcgt cgaggatcgt gttggtgacc ttctcgtgga
     3001 acgcgccctc gtggcggtag ctccagaagt acatcttcag gctcttgagc tcgacgcaga
     3061 gttcgtcggc gatcatgtcg atcgtgaagt gcgcgaagtc cggctggccc gtcaacgggc
     3121 agtggcaggt gaactcgggc acctggaact ggatgacgta gtcgcgcccc ggcgccgggt
     3181 tcgggaagac gtgcaggtcc ttgctcggcg cgctgggcgg gttcggcggc agcgggcgct
     3241 cgttcaggcg ccgtgcggcg cgcgacttgc cgccggacgg gcgggtggtc gccgta
//
LOCUS       DAOQBS010000215         3290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_32870_length_3290_cov_15.762287, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000215 DAOQBS010000000
VERSION     DAOQBS010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32870_length_3290_cov_15.762287"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1761)
                     /locus_tag="PKN25_14965"
     CDS             complement(43..1761)
                     /locus_tag="PKN25_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNV92252.1"
                     /translation="MPPSAAPSVGPQPVSSPAAPWWKSAVAYQLYPRSFMDSDGDGIG
                     DLRGILARLDYLQWLGVDVIWICPIFDSPNDDNGYDIRDYRKILSTFGTMEDFDRLLA
                     EVHRRGMRLILDLVLNHTSDEHPWFLESRASPDNPKRDWYIWREGRDGHPPNNWESIF
                     RGPAWTRDPATGQYYLHLFTRRQPDLDWTNPEMRAAFHDIVRWWLDKGIDGFRLDAVS
                     HIRKMPGLPDLPNPRGLPWVPSFAMHMNVDGVLDTIGELCRETFRRYDVMTVGEANGV
                     GPREAVEWVGSDRGRLDMIFQFEHLALWSRAPGAPLDVVALKKVLSRWQHALHGKGWN
                     ALFLENHDIPRVVSKWGDTGALWRESATALATMYFLMEGTPFIYQGQEIGMTNGVFER
                     IEDFDDVLAKNDYAQRRLAGEEEGAIVADLVLTGRDPARTPMQWDDGPQAGFTRGRPW
                     LAVNPNHRRINVAQQRHDPASVLNHYRRLIALRKAEPALVLGDYRLLMKDDAQIYAYQ
                     RRLDGERIAVIVNLSPHPARFDHPGVVLRHERLLLANRAVEAHPAAHALELAPYEARV
                     YRVGKM"
     gene            2148..3167
                     /locus_tag="PKN25_14970"
     CDS             2148..3167
                     /locus_tag="PKN25_14970"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNV92253.1"
                     /translation="MNATPAPYPRLVADIGGTNARFALVEAPGAAPTHLRALRCAEHS
                     GPEAALRAWLADTGARLPAYAAFGIATPIDGDGVAMTNHPWRFSIGALCGALGLRRLT
                     VVNDFTALALALPALGDGDLVRVGGGEPRAGAARALIGAGTGLGVSGLLPVPGGWVPL
                     QGEGGHVTLPASCTREAAVVAWLAARHGHVSAERVLSGPGLVVLHDTLRALDGEARVE
                     RTPAEISERALAGGCRHCVEALELFCALLGTVAGDVALTLGARGGLYIGGGIVPRLGD
                     FFLRSAFRERFVAKGRFRPWLERIPIWVVVAPHAALTGASAALDSAIELGFTTLAEPR
                     GHVSP"
BASE COUNT          429 a         1133 c         1217 g          511 t
ORIGIN      
        1 gcgtcgatct ccggagcgcc cgtttcgcgc cttgcggcgc gcctacattt ttcccacgcg
       61 gtatacgcgg gcctcgtagg gtgcgagctc gagcgcgtgg gcggcagggt gcgcttcgac
      121 ggcgcggttg gcgagcagca ggcgttcgtg gcggagcacc acgccgggat gatcgaagcg
      181 cgccgggtga gggctcaggt tgacgatcac cgcaatgcgc tcgccgtcca gccggcgctg
      241 gtaggcgtag atctgcgcgt cgtccttcat cagcaggcga tagtcgccga gcaccagcgc
      301 cggctcggcc ttgcgcagcg cgatcaggcg gcggtagtgg ttgagcaccg aagccgggtc
      361 gtggcgctgc tgggcgacgt tgatgcggcg atggttggga ttgaccgcca gccacggccg
      421 tccgcgcgtg aagcccgcct gcggcccgtc gtcccactgc atcggcgtgc gcgccgggtc
      481 gcggccggtg aggacaaggt cggcgacgat cgcgccctcc tcctcgccgg ccaggcggcg
      541 ctgcgcgtag tcgttcttcg ccagcacgtc gtcgaagtcc tcgatgcgct cgaagacgcc
      601 gttggtcatg ccgatctcct ggccctggta gatgaagggc gtgccctcca tcaggaagta
      661 catcgtcgcc agcgcggtcg cgctctcgcg ccacagcgcg ccggtgtcgc cccacttgga
      721 gacgacgcgc gggatgtcgt ggttctccag gaagagcgcg ttccagccct tgccgtgcag
      781 cgcatgctgc cagcgcgaca gcaccttctt gagcgcgacc acgtcgagcg gcgcgccggg
      841 cgcgcgcgac cacagcgcca ggtgctcgaa ctggaagatc atgtcgagcc ggccacggtc
      901 gctgcccacc cactccaccg cctcgcgcgg gccgacgccg ttggcctcgc ccacggtcat
      961 cacgtcgtag cggcggaagg tctcgcggca gagctcgccg atggtgtcga gcacgccgtc
     1021 gacgttcatg tgcatggcga aggacggcac ccagggcagg ccgcgcggat tgggcaggtc
     1081 gggcaggccc ggcatcttgc ggatgtggct caccgcatcg aggcggaagc cgtcgatgcc
     1141 cttgtccagc caccagcgca cgatgtcgtg gaaggccgcg cgcatctccg gattggtcca
     1201 gtcgaggtcg ggctggcggc gggtgaacag gtgcaggtag tactgcccgg tggccgggtc
     1261 ccgggtccac gcggggccgc ggaagatgct ctcccagttg ttgggcggat gcccgtcgcg
     1321 gccctcgcgc cagatgtacc agtcgcgctt ggggttgtcg ggcgaggcgc gcgactcgag
     1381 gaaccagggg tgctcgtcgc tggtgtggtt gagcaccagg tcgaggatca gcctcatgcc
     1441 gcggcggtgg acttcggcga gcaggcggtc gaaatcctcc atggtgccga aggtcgacag
     1501 gatcttgcgg tagtcgcgga tatcgtagcc gttgtcgtcg ttcggggagt cgaagatcgg
     1561 gcaaatccag atcacatcga cgcccagcca ttgcaggtag tcgagccgcg cgaggatccc
     1621 gcgcaggtcg ccgatgccgt cgccgtcgct gtccatgaag ctgcgcgggt agagctggta
     1681 ggcgacggcg gatttccacc agggggcagc ggggctcgaa accggctgcg ggccgacgga
     1741 gggggctgca gaggggggca tgattgcacc gagcaggcgg gttgttcggg aggccgcgtt
     1801 cgattgtagg cccgcgcccg cgggatgacg aacgcaggcc ggacgaaccc gtgcgcctgt
     1861 tgccatcgca ggcgcgacgg aaccttgaat tgctgcgccc ccgccccccc ggctggagca
     1921 ccgggcgacg caggcggaga atcgcgactt gcgtcgctcc tgcggacaag gccggctggc
     1981 ggcgggtagc ccccgtgggc gcgacggggg tcctgatcag ggtgattcgg ccccaccacg
     2041 gcgcggggga acggaatcgg gggacggcga ttacaggcag gtgccttggg gcagctacgg
     2101 acacccgggc cgggcaagcg cctttgcacg tggcagaatc gggggacatg aacgccaccc
     2161 ctgctcccta tccccgcctg gtcgccgaca tcggcggcac caacgcgcgc ttcgccctcg
     2221 tcgaggcgcc cggagcggca ccgacgcatc tgcgagcgct gcgctgcgcg gaacacagcg
     2281 gcccggaggc cgcgttgcgc gcctggttgg cggacaccgg cgcccgcctg cccgcttacg
     2341 ccgccttcgg catcgccacg ccgatcgacg gcgacggcgt cgcgatgacc aatcatccct
     2401 ggcgcttttc gatcggcgcg ctgtgcggcg cgctcggcct gcgccggctg accgtggtga
     2461 acgatttcac cgcgctcgcg ctcgcgctgc ccgcgctcgg cgacggcgac ctggtccgcg
     2521 tcggcggcgg cgagccgcgt gcgggcgccg cgcgggcgtt gatcggcgcc ggcaccgggc
     2581 tcggcgtttc gggcctgctg cccgtgccgg gcggctgggt cccgctgcaa ggcgagggcg
     2641 ggcacgtgac gctgccggcc tcttgcacac gcgaggccgc ggtggtcgcc tggctcgccg
     2701 cccgccatgg ccatgtctcg gccgagcgcg tgctctcggg tcccggtctg gtcgtgctcc
     2761 acgacacctt gcgcgcgctc gacggcgaag cgcgtgtcga gcgcacgccg gcggagatca
     2821 gcgaacgggc gctggccggc ggctgccgcc actgcgtcga ggcactcgag ctcttctgcg
     2881 cgctgctcgg cacggtggcg ggcgacgtcg cgctcaccct gggcgcgcgc ggcgggctgt
     2941 atatcggcgg tggcatcgtg ccgcggctgg gggatttctt cctgcgctcc gcgttccgcg
     3001 aacgtttcgt cgccaagggc cgctttcgcc cctggctcga acgcatcccg atctgggtgg
     3061 tcgtcgcccc ccacgccgcc ctcaccggcg cctcggcggc actcgacagc gccatcgagc
     3121 tcggcttcac cacgctcgcc gagccgcgcg ggcacgtctc cccgtaggcg cgacgcgagt
     3181 cgcgatcggg gcgccgccgc gctcaccgac ggttttcgca tgcgcaatcg tgcggtcgcg
     3241 acttgcgtcg ctcccacgaa aaccggcgcg cgtgctctgt tgccggcatc
//
LOCUS       DAOQBS010000216         3258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_33363_length_3258_cov_9.440212, whole genome shotgun sequence.
ACCESSION   DAOQBS010000216 DAOQBS010000000
VERSION     DAOQBS010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33363_length_3258_cov_9.440212"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PKN25_14975"
     CDS             <1..439
                     /locus_tag="PKN25_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNV92254.1"
                     /translation="LDNRLKEIHFGDWEGRSFDEIGSAIDDWAADPLGFRAPGGESPR
                     EMAARVLAWLEEHGVAARTPDAGSESVLAPKAEIAKAEACPPSPEDPAHLVVVAHGGP
                     LRVLAGYLLGMPPERWIGLDFGCGQVTRIDVESWGTVLKWFNR"
     gene            563..1489
                     /locus_tag="PKN25_14980"
     CDS             563..1489
                     /locus_tag="PKN25_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HNV92255.1"
                     /translation="MTIGIRPDMTILWLITDNKPGHRSQLQGLAQALAARTTVETHWI
                     DAPSGRSALWQWSSGRFPPGAGLPDPDLILVAGHRTHLAGLAARRARGGRLIALMRPS
                     LPLGCFDLCVIPQHDAPPAHAKVIATRGVVNTARPSAERADDRGLFLIGGPSKHHGWD
                     TAGLLAQLDAILVATPAVRWTLTTSRRTPADTEPALLALRERGVDVRPVRDTPPGWAM
                     EQVARSAQAWVSEDSVSMVYESLTAGAATGLLAVPRLGETRIAAGVAALQREGFVTPF
                     VDWQRTGRLSTPPERLAEAERVAEAVLARFGA"
     gene            1713..2306
                     /locus_tag="PKN25_14985"
     CDS             1713..2306
                     /locus_tag="PKN25_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNV92256.1"
                     /translation="MGLALRKEKHYTYADYLQWPDETRYELIDGEAFLMSPAPLVEHQ
                     EVAGEVYYQLRNQLDGQPCRPYIAPVDVRLPRKDEADAAIDTVVQPDVLVVCDPAKID
                     RRGVRGAPDWLLEVLSPSTAAHDQIAKRRTYERAGVREYWLVHPGDRTLTVYLLDNGQ
                     YGRPEIYELKDATPIGVLPGVSIAWDALVARLPKPEY"
     gene            2403..2675
                     /locus_tag="PKN25_14990"
     CDS             2403..2675
                     /locus_tag="PKN25_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HNV92257.1"
                     /translation="MAQSSMLHIRVDDETKTQATEALAAMGLSMSDAVRIFLKRVVND
                     QAFPLELKVPNAETRAAMEEARAMMKAHQARFESTDALFDELEKAR"
     gene            2653..2973
                     /locus_tag="PKN25_14995"
     CDS             2653..2973
                     /locus_tag="PKN25_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HNV92258.1"
                     /translation="MSSKKPAKTKRASLPRAADFSKAFHKDWERFSRSGRFDLKRLKE
                     VMLLLIANDAPLGPEWFDHPLKGEWADHRECHVGGDFLLIYSADDRQITFVRAGTHAD
                     LFEG"
     gene            2981..>3258
                     /locus_tag="PKN25_15000"
     CDS             2981..>3258
                     /locus_tag="PKN25_15000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV92259.1"
                     /translation="MKALKVLQLLPALDSGGVERGTLEIARALVAAGHESVVLSRGGR
                     LVGQLQDEGSRHLARDLGRKSPTTFLHYRALRRLFEAERFDIVHARSRL"
BASE COUNT          540 a         1200 c         1053 g          465 t
ORIGIN      
        1 cctcgacaat cgcctcaagg agatccactt cggtgactgg gaggggcgca gcttcgacga
       61 aatcggcagc gccatcgacg actgggccgc cgatccgctc ggctttcggg cgccgggcgg
      121 cgagtcgccg cgcgagatgg ccgcgcgggt actggcgtgg ctggaggagc atggggtggc
      181 tgcgcggacg ccggatgccg gttcggagtc cgtgcttgcg ccaaaggcgg agatcgcgaa
      241 agccgaggcc tgccccccat ctccagaaga tcccgcgcac ctcgtcgtcg tcgcccacgg
      301 cggcccgctg cgcgtgctcg ccggctacct gctgggaatg ccgcccgagc gctggatcgg
      361 gctcgacttc ggctgcgggc aggtgacgcg gatcgatgtg gaaagctggg gcacggtcct
      421 gaagtggttc aatcgctgag gccgcacgcg cggtgtacaa attcggtatt cggcatttgg
      481 cttgcaagcc gccagggctg cacgtgaacg caagcgtcca tcccattccg tttccacact
      541 cgactgcggt cgaggcctgc aattgaccat cggcatccgc cccgacatga cgatcctctg
      601 gctcatcacc gacaacaagc ccggccaccg cagccagctg cagggcctgg cgcaggcgct
      661 ggcggcgcgc accacggtcg agacgcactg gatcgacgca ccgagtgggc gcagcgcgct
      721 gtggcagtgg tcgagtgggc gcttcccgcc cggcgccgga ctgcccgacc cggacctcat
      781 cctcgtcgcc ggccaccgca cccacctcgc cggcctcgca gcccgacgcg cgcgcggcgg
      841 caggctgatc gcactgatgc ggcccagtct gccgctgggc tgcttcgacc tgtgcgtgat
      901 cccgcagcac gacgcaccgc cggcccacgc caaggtcatc gccacccgcg gcgtggtgaa
      961 caccgcgcgc ccgagcgccg agcgcgccga cgatcgcggc ctgttcctga tcggcgggcc
     1021 gtcgaagcac cacggctggg acaccgccgg cctgctcgcc cagctcgacg ccatcctcgt
     1081 ggccacaccc gccgtgcgat ggacgctcac cacctcgcgg cgcacgcccg ccgacaccga
     1141 acccgccctg ctcgcgctgc gcgaacgcgg cgtggacgtg cgcccggtgc gcgacacccc
     1201 gcccggctgg gcgatggagc aggtcgcgcg cagcgcgcag gcctgggtat cggaagacag
     1261 cgtgtcgatg gtgtacgaga gcctcaccgc cggcgccgcc accggcctgc tcgccgtgcc
     1321 acgcctgggc gagacgcgca tcgccgcagg cgtggccgcg ctgcagcgcg aaggcttcgt
     1381 caccccattc gtcgattggc agcgcaccgg ccgcctgagc acgccccccg agcgcctggc
     1441 cgaagccgag cgcgtggccg aggcggtgct ggcacggttc ggagcgtgag cacaagttcg
     1501 agccccccgc accgcgacgg ttttctgtag gaggcgtcca tataagcgcc gcaaggcgcg
     1561 aaagcggcgg ttgagcttcg tccgcccacg tcgcgcaccc gacggccgta ttcgcggctg
     1621 gcgccgctcc tacacgaaac atcgcgtata caaggcgggc ccgccgccgg cctacaatac
     1681 cgctatcccc cgaaccggat cggagcaccg ccatgggcct cgcgctgcgc aaggaaaagc
     1741 actacaccta cgccgactac ctgcagtggc cggacgagac acgctacgag ctgatcgacg
     1801 gcgaagcctt cctgatgtca cctgcaccgc tggtcgagca tcaggaggtc gccggcgagg
     1861 tctactatca gcttcgcaac cagctcgacg gccagccctg ccgcccctac atcgccccgg
     1921 tcgacgtccg cctgccgcgc aaggacgaag ccgacgctgc catcgacacc gtggttcaac
     1981 ccgacgtgct ggtcgtgtgc gatccggcga agatcgaccg ccgtggtgtg cgcggcgcgc
     2041 ccgactggct gctcgaggtg ctctccccca gcaccgccgc ccacgaccag atcgccaagc
     2101 gccgcaccta cgagcgcgcc ggcgtgcgcg aatactggct ggtgcacccg ggggaccgca
     2161 ccctcactgt ctatctgctc gacaacggcc agtacggccg gccggagatc tacgagctga
     2221 aggacgcgac cccgatcggc gtgctgccgg gcgtgtcgat cgcctgggac gcgctggtcg
     2281 cacgcctgcc caagccggag tattgatctg gccgacccgg cttcgctccc gccacaggaa
     2341 ccgcctggct tgatcgcatc tcatatgcga tacataattc gcactccgca agcgaggaag
     2401 ccatggcaca gagttcaatg ctccacatcc gcgtcgacga cgaaacgaag acccaggcca
     2461 ccgaggcgct ggccgccatg ggcctgtcga tgtccgacgc cgtgcgcatc ttcctcaagc
     2521 gcgtcgtcaa cgaccaggcc ttcccgctcg aactcaaggt ccccaacgcc gaaacgcgcg
     2581 ccgccatgga agaggcgcgc gccatgatga aggcacacca ggcacgtttc gaatcgaccg
     2641 acgccctctt cgatgagctc gaaaaagccc gctaaaacca agcgagcatc gctgccgcgc
     2701 gccgcggatt tcagcaaggc cttccacaag gactgggaac gattcagccg ctccggccgt
     2761 ttcgacctca agcggctcaa ggaagtcatg ctgctgctca tcgccaacga cgccccgctc
     2821 ggcccggagt ggttcgacca cccgctcaag ggcgagtggg ccgatcaccg cgaatgccac
     2881 gtcggcggcg acttcctgct catctacagc gccgatgacc gacagattac cttcgtccgg
     2941 gcgggcaccc atgcggatct cttcgaggga tgaggcacga atgaaagccc tcaaggtcct
     3001 gcaacttctc cccgcgctgg acagcggcgg cgtcgagcgt ggcacgctcg agatcgcgcg
     3061 cgcgctggtc gcggccgggc acgaatcggt ggtgctgtcc aggggcgggc gcctggtcgg
     3121 gcagttgcag gacgaaggct cgcgccacct cgcgcgcgac ctcgggcgca agtcgccgac
     3181 caccttcctg cactaccgcg cgctgcgcag gctcttcgag gccgagcgct tcgacatcgt
     3241 ccatgcccgc tcgcgcct
//
LOCUS       DAOQBS010000217         3239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_33683_length_3239_cov_17.538317, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000217 DAOQBS010000000
VERSION     DAOQBS010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33683_length_3239_cov_17.538317"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /gene="urtD"
                     /locus_tag="PKN25_15005"
     CDS             <1..616
                     /gene="urtD"
                     /locus_tag="PKN25_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HNV92260.1"
                     /translation="GKTTMMDVITGKTRPDSGTAFFGQTIDLTRLTEPEIAHAGIGRK
                     FQKPTIFEQHTAFENLELAMKADKRVRRTLFASLIDDERDRVAAVLRQIRLDQAADRP
                     AGLLSHGQKQWLEIGMLLMQEPRLLLLDEPVAGMTDDETERTAELFTSLAGKHSLVVV
                     EHDMAFVEALGGKVTVLCEGSVLAEGDLATVQADPRVIEVYLGR"
     gene            623..1315
                     /gene="urtE"
                     /locus_tag="PKN25_15010"
     CDS             623..1315
                     /gene="urtE"
                     /locus_tag="PKN25_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HNV92261.1"
                     /translation="MLKVDNLNQYYGGSHILRGLSFDVPVGKVTTLLGRNGVGKTTLL
                     KTLMGLVPAASGSISFHDQDITRAPSWKRVAAGIGFVPQGREIFPRLTVEENLLMGLA
                     TRPRGSAIPPRIFDMFPVLRQMMGRRGGDLSGGQQQQLAIGRALAFGPRLLILDEPTE
                     GIQPSIIKDIERAIRSLAETGEMAILLVEQYYDFARALADQYLVMERGEIVMRGAGAD
                     MDRDGVREALAV"
     gene            1534..2412
                     /locus_tag="PKN25_15015"
     CDS             1534..2412
                     /locus_tag="PKN25_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HNV92262.1"
                     /translation="MYPPLPPLASSLLPPAGAAVSTPWRAGLSLEYEKRGARSVLARR
                     EHFGPLRVQRALYPEGEAVCHTLLVHPPAGIVGGDELRVDLRIGAGAHVLLTTPGAGK
                     WYRSAGARGVLAQHIAVATGAVCEWLPQESIVHEGALAGLATEVRLEGDACFIGSEML
                     CFGRTASGERFAHGELAMRTRIERDGRPVWLERGVVHGGGGLLAAAPGLHGQPVSASL
                     LIASPRCDEALRDAWRALSPAAGEGAVSLLPGVLVARWLGPACEPGRQWFTRLWATAR
                     PAVAGRAAHTPRIWNT"
     gene            2451..2753
                     /gene="ureA"
                     /locus_tag="PKN25_15020"
     CDS             2451..2753
                     /gene="ureA"
                     /locus_tag="PKN25_15020"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HNV92263.1"
                     /translation="MELSPREKDKLLVFTAGLLAERRRARGLKLNYPEAVALITCAIL
                     EGARDGRTVAELMGYGATLLTRADVMEGVAEMIPDIQVEATFPDGTKLVTVHNPIV"
     gene            2765..3118
                     /locus_tag="PKN25_15025"
     CDS             2765..3118
                     /locus_tag="PKN25_15025"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HNV92264.1"
                     /translation="MIPGELFVADGDIELAAGRATRTLVVSNTGDRPIQVGSHYHFAE
                     TNSALDFDRAAARGFRLNIAAGTAVRFEPGQWRTVELVALDGEREVYGFNGLVMGRLD
                     EPATAPAAHRTQGSL"
     gene            3118..>3239
                     /locus_tag="PKN25_15030"
     CDS             3118..>3239
                     /locus_tag="PKN25_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92265.1"
                     /translation="MATISRRAYAEMFGPTVGDRLRLADTELVIEVEKDFTIYG"
BASE COUNT          476 a         1188 c         1153 g          422 t
ORIGIN      
        1 cggcaagacc accatgatgg acgtcatcac cggcaagacg cggcccgact cgggcaccgc
       61 cttcttcggc cagaccatcg acctcacccg cctgaccgaa cccgagatcg cccacgccgg
      121 catcggccgc aagttccaga agccgaccat cttcgagcag cacacggcct tcgagaacct
      181 cgagctggcc atgaaggccg acaagcgcgt gcgccgcacc ctgttcgcca gcctcatcga
      241 cgacgagcgc gaccgcgtcg ccgcggtgct gcgccagatc cgcctcgacc aggccgccga
      301 ccgccccgcc gggctgctct cgcacggcca gaagcagtgg ctggagatcg gcatgctgct
      361 gatgcaggag ccacgcctgc tgctgctcga cgagccggtc gccggcatga ccgacgacga
      421 gaccgagcgc accgccgagc tgttcacgag cctcgccggc aagcactcgc tggtggtggt
      481 cgagcacgac atggccttcg tcgaggcgct cggcggcaag gtcacggtgc tgtgcgaggg
      541 ttcggtgctg gccgaggggg atctcgccac ggtgcaggcg gatccgcgcg tgatcgaggt
      601 ctacctgggg cgctgaacga acatgctgaa agtcgacaac ctgaaccagt actacggcgg
      661 cagccacatc ctgcgcggcc tgtccttcga cgtgcccgtc ggcaaggtga cgacgctgct
      721 cgggcgcaac ggcgtgggca agaccacgct gctgaagacc ctgatgggcc tggtgcccgc
      781 cgccagcggc agcatttcct tccacgacca ggacatcacc cgcgcgccgt cctggaagcg
      841 cgtcgccgcc ggcatcggct tcgtcccgca ggggcgcgag atcttcccgc gcctcaccgt
      901 cgaggagaac ctgctcatgg gcctggccac ccggccacgc ggcagcgcca tcccgccgcg
      961 catcttcgac atgttcccgg tgctgaggca gatgatgggc cggcgcggcg gcgacctctc
     1021 cggcggccag cagcaacagc tcgcgatcgg ccgcgcgctc gccttcggcc cccggctgct
     1081 gatcctcgac gagcccaccg agggcatcca gccctccatc atcaaggaca tcgagcgcgc
     1141 catccgcagc ctcgccgaga ccggcgagat ggccatcctg ctcgtcgagc agtactacga
     1201 cttcgcgcgc gcgctcgccg accagtacct ggtaatggag cgcggcgaga tcgtcatgcg
     1261 cggcgcaggg gcggacatgg accgcgacgg ggtgcgcgag gcgctcgcgg tgtgagggtc
     1321 gccgcgtcgc ggggcgcggc gggtccggcg ggtctgcgcg gtcgcgctgc gcagccgcac
     1381 cgcgctggtg cgcccacagg cgaacgcacc aggacggggc atcgatgggt ctccgccgcg
     1441 ccgcgcagcc ccaccgtcgc acccgcaaca cccctgtcag gcgcagccat cgggcgttca
     1501 gagagctggc ccgggtcttg ctaaacctcc cccatgtacc cgccgctgcc tcccctcgcc
     1561 tcttcgctat tgccccccgc cggcgccgcg gtatccacgc cctggcgcgc cgggctgagc
     1621 ctggaatacg agaagcgcgg cgcacgcagc gtgctcgcac gccgtgagca cttcggcccg
     1681 ctgcgcgtgc agcgcgcgct ctacccggaa ggcgaggccg tctgccacac cctgctggtg
     1741 cacccgcccg ccggcatcgt cggcggcgac gagctgcggg tggacctgcg catcggcgcg
     1801 ggcgcgcacg tgctgctgac caccccgggc gccggcaagt ggtaccgcag tgccggcgcg
     1861 cgcggcgtgc tcgcgcagca catcgcggtg gcgacgggcg cggtgtgcga gtggctgccg
     1921 caggagagca tcgtccatga aggcgcgctc gccgggctcg ccaccgaggt gcgcctggaa
     1981 ggcgatgcct gcttcatcgg cagcgagatg ctgtgcttcg gccgcacggc ctccggcgaa
     2041 cgcttcgccc acggcgagct ggcgatgcgc acccgcatcg agcgcgacgg ccggccggtc
     2101 tggctggagc gcggcgtcgt gcatggcggt ggcgggctgc tcgccgccgc gcccggcctg
     2161 cacggccagc cggtgagcgc gagcctgctg atcgcctcgc cgcgctgcga cgaggccttg
     2221 cgcgatgcct ggcgcgcgct ctcgcccgcg gccggcgaag gcgcggtgag cctgctgccg
     2281 ggcgtgctgg tggcgcgctg gctggggccg gcctgcgagc ccggccggca gtggttcacc
     2341 cgcctgtggg cgacggcgcg cccggcagtc gcgggacggg cggcgcacac gccgcggatc
     2401 tggaacacct gaaacccatg ggcggcagcg cgccgccgcg gaggaagacg atggaactga
     2461 gcccacgcga gaaggacaag ctgctggtgt tcaccgccgg cctgctcgcc gaacgccggc
     2521 gcgcgcgcgg cctgaagctg aactacccgg aggcggtggc gctgatcacc tgcgcgatcc
     2581 tcgagggcgc gcgcgacggc cgcacggtgg cggagctgat gggctacggc gcgaccctgc
     2641 tgacgcgcgc ggacgtgatg gagggcgtcg cggagatgat ccccgacatc caggtggagg
     2701 cgacgtttcc cgacggcacc aagctggtga ccgtgcacaa cccgatcgtg tgaggagcag
     2761 gccgatgatc cccggagaac tcttcgtcgc cgacggcgac atcgaactcg ccgccggccg
     2821 tgccacgcgc acgctggtgg tgagcaacac cggcgaccgc ccgatccagg tcggctcgca
     2881 ctaccacttc gccgagacca acagcgcgct cgacttcgac cgcgcggcgg cgcgcggctt
     2941 ccgcctgaac atcgccgccg gcacggcggt gcgcttcgag cccggacagt ggcgcaccgt
     3001 cgagctggtg gcgctcgatg gcgagcgcga ggtgtacggc ttcaacggcc tcgtcatggg
     3061 acggctggac gagcccgcga ccgcgcccgc cgcgcaccgg acgcaaggga gcctgtgatg
     3121 gcgacgatct cgcgccgcgc gtacgcggag atgttcggcc ccacggtggg cgaccggctc
     3181 cgcctggccg acaccgagct ggtgatcgag gtggagaagg acttcaccat ctacggcga
//
LOCUS       DAOQBS010000218         3199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_34290_length_3199_cov_14.615776, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000218 DAOQBS010000000
VERSION     DAOQBS010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34290_length_3199_cov_14.615776"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1509
                     /locus_tag="PKN25_15035"
     CDS             211..1509
                     /locus_tag="PKN25_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNV92266.1"
                     /translation="MPVNELLVILLLIAASAFFSMSEISLAAARKIKLRVMAEAGHLN
                     ARRVLALQDSPGHFFTVVQIGLNAVAILGGVVGEQALSPYVTELLRRAYAGPMLDTIA
                     FVVSFVFVTSLFVLFADLMPKRLAMVQPERIAVAVVQPMQVCMWLFAPLVWVFNGAAN
                     LIFRWFKLPSVRIEDITADDIMAMADAGAQAGALLRQEQHLISNVFELDSRIVPSAMT
                     SRENIVFLTLSESEESIRRKIAAHPHGKFPVCEDGIDSVIGYVDSKDILPRIVQGQDL
                     SLRTQPIVRKVLMLPDTLTLFEALERFRDAKEDFALILNEYALVVGLLSLQDVMNTVM
                     GDLVSPFQEELIVRRDDNSWLIDGATPIEDVMQALEIEVFEGFQNYETVAGFLMYRLR
                     KVPKRTDFVTYLGYKFEVVDIDNYRIDQVLVTRETPVGAV"
     gene            complement(1696..2367)
                     /locus_tag="PKN25_15040"
     CDS             complement(1696..2367)
                     /locus_tag="PKN25_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNV92267.1"
                     /translation="MNATRIAPIALYQEVAERLRQRIFSHELPPGTWVDEQALAEHYG
                     ISRTPLREALKVLASEGLVTLKPRRGCYVTEISERDLDEVFSVMSMLEGECARLSAAR
                     ATEDDLARLRAIHANLEKAAGKPDIDRFFEANQAFHLALQEIADNRWLLHVIEDLRKV
                     IKLSRHHSLFSEGRLEQSLAEHRDILQALLDRDGARAETLMRTHILSGRAALARIAEA
                     KTQAA"
     gene            2778..>3199
                     /locus_tag="PKN25_15045"
     CDS             2778..>3199
                     /locus_tag="PKN25_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNV92268.1"
                     /translation="MSKANDPVYPQPDIEAWKKAAAKQAPGGDLDKLNWITPEGLSVK
                     PLYTKADTEGLQHADTLPGFEPFLRGPQPTMYAVKPWTIRQYAGFSTAEASNAFYRKA
                     LAAGGQGVSVAFDLATHRGYDSDHPRVTGDVGKAGVAI"
BASE COUNT          498 a         1089 c         1053 g          559 t
ORIGIN      
        1 ggcgggcgat gcgcggccgg aaagggctgc gggggcgcaa tcgcgacccg cgtcgctcct
       61 acattcctcc tgcattcctc ctccatccct tcccgggggg ggggcggcga tgcgccgggg
      121 caccgattgc ccttcaggcc gcggtggtag actcgcagac tagattctgg ttttgccttc
      181 cggcgggacc ttcatcccaa aggagtcgtc gtgcctgtca acgaactgct cgtcatcctg
      241 ctgctgatcg cagcgagcgc cttcttttcc atgtccgaga tctccctcgc ggctgcgcgc
      301 aagatcaagc tgcgcgtcat ggccgaggca gggcatctca acgcccggcg cgtgcttgcg
      361 ctgcaggaca gtccgggcca cttcttcacc gtggtgcaga tcggcctcaa cgccgtcgcc
      421 attctcggcg gcgtagtcgg cgaacaggcg ctttctccct acgtcaccga gctgctgcgc
      481 cgggcctacg ccggcccgat gctcgacacc atcgccttcg tcgtctcctt cgttttcgtc
      541 acctcgctgt tcgtgctttt tgccgacctc atgcccaagc gcctggccat ggtccagccc
      601 gaacgcatcg cggtggcggt ggtgcagccg atgcaggtgt gcatgtggct gttcgcgccg
      661 ctggtgtggg tcttcaacgg cgcggccaac ctcatcttcc gctggttcaa gttgccgagc
      721 gtgcgcatcg aggacatcac cgccgacgac atcatggcca tggccgacgc cggtgcccag
      781 gccggcgcgc tgctgcgcca ggagcagcac ctgatcagca acgtgttcga gctcgactcg
      841 cgcatcgtgc cctcggcgat gacctcgcgc gagaacatcg tcttcctcac cctgtcggag
      901 tccgaggaga gcatccggcg caagatcgcc gcccacccgc acgggaagtt tccggtgtgc
      961 gaggacggca tcgacagcgt gatcggctat gtggactcga aggacatcct gccgcgcatc
     1021 gtgcagggcc aggatctgtc cttgcgcacc cagccgatcg tgcgcaaggt gctgatgctg
     1081 cccgacacgc tcacgctctt cgaggcgctc gagcgcttcc gcgacgccaa ggaggatttc
     1141 gcgctcatcc tcaacgaata cgcgctggtg gtgggcctgc tgtcgctgca ggacgtgatg
     1201 aacacggtga tgggcgatct cgtcagcccc ttccaggaag agctcatcgt gcgccgagac
     1261 gacaactcct ggctcatcga cggcgccacg ccgatcgagg acgtcatgca ggcgctcgag
     1321 atcgaggtct tcgagggctt ccagaactac gagaccgtcg ccggtttcct gatgtaccgc
     1381 ctgcgcaagg tccccaagcg caccgacttc gtgacctacc tcggctacaa gttcgaggtg
     1441 gtcgacatcg acaactaccg catcgaccaa gtgctggtca cccgcgagac cccggtcggc
     1501 gccgtgtaac aacaagccgg ttccgacgcc cgtcgtccgc gtcggcgcct gtcgggtgcg
     1561 gaatcgccgc aatcgcgact tgcgtcgctc ccacgaaaac cggcggcggc ttcggtggga
     1621 tgtaggagcg acgcgagtcg cgattgcggg cgtgtcggcc gggtcgctcc cacacgaaat
     1681 caatgtggcc aggcctcacg cggcctgcgt cttcgcctcg gcgatgcggg cgagggcggc
     1741 gcggccgctg aggatgtggg tgcgcatcag ggtctcggcg cgcgcgccgt cgcggtcgag
     1801 caaggcctgg aggatgtcgc ggtgctcggc gagcgactgc tccagccggc cctccgagaa
     1861 cagcgagtgg tggcgcgaca gcttgatcac cttgcgcagg tcctcgatca cgtggagcaa
     1921 ccagcgattg tcggcgatct cctgcagggc gaggtggaag gcctggttgg cctcgaagaa
     1981 gcggtcgatg tcgggcttgc cggcggcctt ctcgaggttg gcgtggatcg cgcgcaggcg
     2041 ggcgaggtcg tcctcggtcg cgcgcgcagc cgacagccgg gcgcactcgc cttcgagcat
     2101 cgacatcacg ctgaacacct cgtcgagatc gcgctccgag atctcggtca cgtagcagcc
     2161 gcgccgcggc ttgagcgtca ccaggccctc ggaggccagt accttcagcg cctcgcgcag
     2221 cggtgtgcgc gagatgccgt agtgctcggc cagcgcctgt tcgtccaccc aggtgccggg
     2281 cggcagctcg tgcgagaaga tccgctggcg caggcgctcg gccacttcct ggtagagcgc
     2341 gatcggagcg atgcgggtgg cgttcatggg cggattcttt cttcggagcg gattcaaaag
     2401 gtggcgcggg gcagggccat cacgggtggt ggtggcgcgt gcatgccctc tctcgccgga
     2461 cgggcccggc tcgctgcgcg ccctttccga ccgtcggaac gcattcgccc gctgccgcct
     2521 attgcattca taattatgga taaagtatta tttttcctgc agtgcaagtc aacttccgaa
     2581 gcgcgatgcg tcatgggagg cgccgggacc acccggcgga agagagggga cgcgtcgctt
     2641 gccggagcgt cgacggccgc tcgtgggatc gccacgggcg gcttcgtcgc cgccccgggg
     2701 gggtttggct tgccgataaa tcaccaatcc gggtcgcgcc tgtaccccag gccgccgcag
     2761 atctcacgag gtttcccatg tcgaaagcca acgatcccgt ctatccccag cccgacatcg
     2821 aggcctggaa aaaggctgcc gccaagcagg cgccgggggg cgacctcgac aagctgaact
     2881 ggatcacgcc cgagggcctc tcggtcaagc cgctgtacac caaggccgac accgaaggcc
     2941 tgcagcacgc cgacacgctg cccggcttcg agcccttcct gcgcggcccg cagcccacca
     3001 tgtacgcggt caagccgtgg accatccgcc agtacgccgg cttctccacc gccgaagcgt
     3061 ccaacgcctt ctaccgcaag gcgctcgccg ccggcggcca gggcgtgtcg gtcgcgttcg
     3121 acctggcgac ccaccgcggc tacgactccg accatccgcg cgtgaccggc gacgtcggca
     3181 aggccggcgt ggcgatcga
//
LOCUS       DAOQBS010000219         3181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_34586_length_3181_cov_17.291747, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000219 DAOQBS010000000
VERSION     DAOQBS010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34586_length_3181_cov_17.291747"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..613)
                     /locus_tag="PKN25_15050"
     CDS             complement(2..613)
                     /locus_tag="PKN25_15050"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HNV92269.1"
                     /translation="MKLLASLTSPYARKIRVILAEKALPFELVVDSPWEANTRIPELN
                     PLGKVPVLITDEGETFFDSPVIAGYLETLGAPPALLPPAGLDAVRVRQTEALADGVTD
                     AAVAALLESRRPDGERSEREIARQKDKIDRGLAELERRLDGRHAFNGGPMQLGDIAVA
                     VALGYLDLRFPDVDWRSRHPGLVPFAERMFARSSLRDTQPPAG"
     gene            complement(845..1501)
                     /locus_tag="PKN25_15055"
     CDS             complement(845..1501)
                     /locus_tag="PKN25_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNV92270.1"
                     /translation="MSRPLPTESALLRGPAGNIEALIDAPATVKGIALVCHPHPLFGG
                     ANTNKVAHTLARAFRDLGYAVIRPNFRGVGQSEGTHDHGEGETEDMLSVISWAESRWG
                     ALPLALGGFSFGGYVQVRVAKRLAEGIAPPRQLVLVGMAAGETTGSGRSYDTPALPTN
                     IPALVIHGENDDTVALANVLDWARPQEQPIIVIPGADHFFHGKLHLIRELIARNVAHA
                     "
     gene            complement(1498..1821)
                     /locus_tag="PKN25_15060"
     CDS             complement(1498..1821)
                     /locus_tag="PKN25_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNV92271.1"
                     /translation="MSYFKHHVFFCCNQRQPGESCCNDHKASELQTYAKERTAALGLK
                     GKGSVRVNKAGCLGRCDDGPVIVVYPDNVWYTYVDKEDIDEIIDSHLMQGRVVQRLRL
                     PEDAA"
     gene            complement(1978..3105)
                     /locus_tag="PKN25_15065"
     CDS             complement(1978..3105)
                     /locus_tag="PKN25_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNV92272.1"
                     /translation="MRRMAARSPSSLAPAFALAYALLVAYACLHPLTGWRDSGLPAFD
                     WLWAPWPKYFILEDLLFNILGYLPLGLLLAAALPAQWRFARKALVAALLAGLFSFGLE
                     ALQNYLPSRVASNLDLGGNAAGGLLGALAGARWGSALLGPQGRLQRWRARSVIGGRSG
                     EAGLVLIGLWLLGQLGATDLVFASGDLRSLLGIPAPLPFRPERFIAFDTALTASGLLA
                     IGLFVRCMTRGASPLPVLAVIALGIGAKSLATWIFFEPGAPLAWLTPGAERGLVIGGA
                     LLLPALLLPRLAQHAIAGTSLLLATALVNLIPENPYLPFDRRLAGFSNVFSFHGLTGL
                     VDSLWPYAALAYLSALGLWRGEHLAAAPASAQPAPRRRARR"
BASE COUNT          442 a         1039 c         1269 g          431 t
ORIGIN      
        1 cctagcccgc cggcggctgg gtgtcgcgca ggctgctgcg ggcgaacatg cgctcggcga
       61 agggcaccag gccggggtgg cggctgcgcc agtcgacatc ggggaagcgc aggtcgaggt
      121 agccgagcgc gaccgcgacc gcgatgtcgc cgagctgcat cgggccgccg ttgaaggcgt
      181 ggcggccgtc gaggcggcgt tcgagctcgg cgaggccgcg gtcgatcttg tccttctggc
      241 gggcgatctc gcgttcgctg cgctcgccgt cggggcggcg cgattcgagc agggcggcga
      301 ccgccgcgtc ggtgacgccg tcggcgagcg cctcggtctg gcgcacgcgc accgcgtcga
      361 gcccggcggg cgggagcagc gcgggcgggg cgccgagggt ctcgaggtag ccggcgatga
      421 ccggcgagtc gaagaaggtc tcgccttcgt cggtgatcag caccggcacc ttgccgagcg
      481 ggttgagctc ggggatccgc gtgttggcct cccagggcga gtcgaccacg agctcgaagg
      541 gcagcgcctt ctcggcgagg atgacgcgga tcttgcgcgc gtaggggctg gtcagcgagg
      601 cgagcagttt catgatggcg ggtctccggt gatggccgac ccagcgtacg ccgcaggcgg
      661 gcggggggaa atcagggaaa ccggaaggat cgcggcccgc gtcgcgagaa ggccggcggg
      721 gcgcggaggc gggcgcggcg cggagtccgg tgctggaatc gcgacttgcg tcgctcctac
      781 ggcaatcttg taagcgcctg ggtttcgtag gagcgacgca agtcgcgatt gccggcggca
      841 gtgttcaggc gtgcgcgacg ttgcgcgcga tgagctcgcg gatcaggtgc agcttgccgt
      901 ggaagaagtg gtcggcgccg gggatcacga tgatcggctg ttcctgcggc cgcgcccagt
      961 cgagcacgtt ggcgagcgcg acggtgtcgt cgttctcgcc gtggatgacc agcgcgggga
     1021 tgttcgtcgg cagcgccggg gtgtcgtagc tgcggccgct gccggtggtc tcgcccgcgg
     1081 ccatgcccac gagcacgagc tggcgcggcg gcgcgatgcc ctcggccagg cgcttggcga
     1141 cgcgcacctg tacgtagccg ccgaaggaga agccgccgag cgccagcggc agcgcgcccc
     1201 agcgcgactc cgcccagctg atcaccgaga gcatgtcctc ggtctcgcct tcgccgtggt
     1261 cgtgcgtgcc ctcgctctgg cccacgccgc ggaagttggg gcggatcacc gcgtagccga
     1321 gatcgcggaa ggcgcgcgcg agcgtgtgcg cgaccttgtt ggtgttggcg ccgccgaaga
     1381 gcgggtgggg atggcacacg agcgcgatgc ccttgaccgt ggcgggggcg tcgatcagcg
     1441 cctcgatgtt gccggcgggg ccgcgcagca gggcggactc ggtgggcagc gggcggctca
     1501 tgcggcgtcc tcgggcaggc gcaggcgctg gaccacgcgg ccctgcatca ggtgcgagtc
     1561 gatgatctcg tcgatgtctt ccttgtcgac gtaggtgtac cagacgttgt cggggtagac
     1621 gacgatcacc ggaccgtcgt cgcagcggcc gaggcagccc gccttgttga cgcgtacgct
     1681 gcccttgccc ttgaggccga gcgcggcggt gcgttccttg gcgtaggtct gcagctcgct
     1741 cgccttgtgg tcgttgcaac aggactcgcc cggctggcgc tggttgcagc agaagaagac
     1801 gtggtgtttg aagtagctca tggtggctct ctgggtatcg ggtcgaacgc cgtgccggcg
     1861 ccgatccgcg ggaacgtcgc gagattatag gcggcgcttg ccgcgtgtgc ggcttgcggc
     1921 ggggttgtgg ccgcgggacc tggccgcggg gcctggctgc ggccgccgcc ccgatgccta
     1981 gcggcgggct cggcggcgcg gagccggctg cgcgctcgcg ggggcggccg cgaggtgctc
     2041 gccccgccag agcccgagcg ccgacaggta ggccagcgcg gcgtagggcc acaggctgtc
     2101 gaccagcccg gtgagtccgt ggaagctgaa cacgttgctg aacccggcca ggcgccggtc
     2161 gaagggcagg taggggttct ccgggatcag gttcaccagc gcggtggcga gcagcaggct
     2221 ggtgccggcg atcgcgtgct gggccaggcg cggcagcagc agcgcgggca gcagcagcgc
     2281 gccgccgatc accaggccgc gctcggcgcc cggggtgagc caggcgagcg gcgcccccgg
     2341 ctcgaagaag atccacgtcg ccagcgactt cgcgccgatg ccgagggcga tcaccgccaa
     2401 caccggcagc gggctggccc cgcgcgtcat gcagcgcacg aacagtccga tcgcgagcag
     2461 cccgcttgcg gtgagcgcgg tgtcgaaggc gatgaagcgc tcggggcgga agggcagcgg
     2521 cgcagggatg ccgagcaggc tgcgcaggtc gccgctggcg aagacgaggt cggtggcgcc
     2581 gagctggccg agcagccaca gcccgatcag caccagtccg gcctcgccgc tgcgcccgcc
     2641 gatcacgctg cgcgcgcgcc agcgctgcag cctcccctgc ggcccgagca gcgcactgcc
     2701 ccagcgcgcg ccggcgagtg cgccgagcag gccgccggcc gcgttgccgc cgaggtcgag
     2761 attgctcgcc acccggctgg gcaggtagtt ctgcagcgcc tccaggccga agctgaacag
     2821 cccggcgagc agcgcggcga cgagcgcctt gcgcgcgaag cgccactgcg ccggcagcgc
     2881 cgccgccagc agcagcccga gcggcaggta gccgaggatg ttgaagagga gatcttcgag
     2941 gatgaagtac ttcggccatg gcgcccacag ccagtcgaac gccggcaagc cgctgtcgcg
     3001 ccagccggtc agcggatgca ggcaggcata ggcgaccagc agcgcatagg cgagcgcgaa
     3061 cgcgggagcg agggaggagg gcgaccgtgc ggccatgcgc cgcattgtgc cagctgggcc
     3121 ggatcgcagc accccgggac ggggtgtcca tgcgctcgag ctccggtgcc acgctacagg
     3181 g
//
LOCUS       DAOQBS010000220         3115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_35728_length_3115_cov_13.645098, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000220 DAOQBS010000000
VERSION     DAOQBS010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35728_length_3115_cov_13.645098"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PKN25_15070"
     CDS             complement(<1..381)
                     /locus_tag="PKN25_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNV92273.1"
                     /translation="MFEAVRNNKRVAQVILALLIVPFAFFGMDAYFSDSGSANEAARV
                     GGTTIGAWEFDQALREQQDRLRQDGGGQVDRALLQSAELRRAVLENLINQRVLALYAA
                     ENRLVVTPQQLQETIAGVASFQENG"
     gene            533..>3115
                     /locus_tag="PKN25_15075"
     CDS             533..>3115
                     /locus_tag="PKN25_15075"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV92274.1"
                     /translation="MRASPLPAVVLALAYWAVGYPALHLAIPPGYTAPFFPPAGIALA
                     ALFVFGLRLWPAVFAGSALVQLATAWPLLGSAGWNPLGPLAVPVGATLQAVVGVLLAR
                     RLLAPGDTLDTAGSVMRFLGICMPLSGLVCSTIGVSALVWAAVIPASDALFNWWTWWT
                     GDTLGMLVAAPLTLVFIGRPAQDWRQRRLGVALPMSIALVLLALAYRQVVSWEDSRVR
                     AQFERDSVHLSALLQKRLEEQVDMVRSIERLLLASDQVSRADFHEFVAPWMRLHPGTR
                     SFTWNPYVRHAERAAFEEGIRSDCRVDYRILDRDPAGQTSPATERREYFPVTHVEPEE
                     GNHAVLGMDPLSFPVAGEAIERALATGQPAASARMRLVQEHGEQHAVVLYQAVFASSP
                     PAPVRAPLGVVSAALGMDDLLAAVSRQAAAQGIAVRLSDRDAGPESALLAGDDRGQES
                     VWPEEILARTTSIDFAGRRWELHTRALPRYIEQLRSWAAWGTIAVGVLTSAMLGALLL
                     ITTGNRRRIQALVEHRTAELQAASVRLHENRAALIDAQRIARLGSWETLCARDGLHAS
                     DELHLLLDSTAGDLNSLADLIDAIAPASRAALTDALERAATEPGRIMLDCNTDSLPMR
                     TLQFRIEGEHVGGRLRRIRGTAQDVTALREAEARIRYLARFDPLTGLLNRSAWQDHAQ
                     DVLRHAARHDDRCAVLFLDLDDFKTVNDSLGHAAGDRLLAQVACRLGGCVRAEDLVAR
                     IGGDEFVILLSRLAHAEEPARVAERVLDALARPLQIDSQPLRVGASIGIALYPADGGE
                     IDTLLKHADTAMYEAKESGRNAYRFFLPEMTQRVTRRLQTEAELRKAIESGDLRLHYQ
                     PQIDA"
BASE COUNT          450 a         1164 c         1019 g          482 t
ORIGIN      
        1 gccgttttcc tggaaggagg ccacgccggc gatggtctcc tgcagctgct gcggcgtcac
       61 cacgagacgg ttctcggcgg cgtagagcgc gagcacgcgc tggttgatca ggttctcgag
      121 cacggctcgc cgcagttcgg ccgactgcag cagcgcacgg tcgacctggc ccccgccgtc
      181 ctgccgcagg cggtcctgct gctcgcgcag ggcctggtcg aactcccatg ctcctatggt
      241 ggtgccgccg acacgcgcgg cctcgttcgc cgaacccgag tcgctgaagt aggcgtccat
      301 gccgaagaag gcgaagggga cgatcaggag cgccaggatc acctgggcga cgcgtttatt
      361 gttgcgaaca gcctcgaaca tctcgtattc cgtggaaagg acagaaaaat gcagccgtgt
      421 ggcaggatcc cggcgcgaaa agttccgcag tttagcgcat cggtccggct tcgggtccgc
      481 ggcatgccat ccgtcacagc gcgcagggaa cggcgctcaa tatactgcgc ggatgcgcgc
      541 aagccctctc cccgcagtcg tcctcgccct ggcctattgg gcggtcggct atcccgccct
      601 ccatctcgcc atcccaccgg gctacaccgc ccccttcttt ccaccggccg gtatcgcact
      661 cgccgcgctg ttcgtgttcg gactgcgcct gtggcctgcg gtgttcgcag ggtcggccct
      721 ggtccagctc gccaccgcct ggccgctgct cggcagcgca ggctggaatc cgctcggtcc
      781 gctcgccgtc ccggtaggcg ccaccctgca ggcggtggtc ggcgtcctgc tcgcgcgccg
      841 gctgctcgcg cccggcgaca cgctcgacac ggccggctcg gtgatgcgct tcctcggcat
      901 ctgcatgccg ctgagcgggc tggtgtgctc gacgatcggc gtctccgccc tggtctgggc
      961 ggcggtgatc ccggcatccg acgcgctctt caactggtgg acctggtgga ccggcgacac
     1021 cctgggcatg ctcgtcgccg ccccgctcac cctggtgttc attggtcgcc cggcgcagga
     1081 ctggcgccag cgtagactcg gcgtcgcgct gccgatgtcg atcgcgctcg tgctgctcgc
     1141 cctcgcctac cggcaggtgg tgagctggga ggattcgcgc gtgcgggcgc agttcgagcg
     1201 cgacagcgtc catctctccg ccctgctgca gaagcgcctc gaagaacagg tcgacatggt
     1261 gcgttcgatc gagcgcctgc tgctcgcgag cgaccaggtc tcgcgcgccg atttccacga
     1321 gttcgtcgcg ccctggatgc gactgcaccc gggcacgcgc agcttcacct ggaaccctta
     1381 cgtaagacac gctgagcgcg cggcattcga agaggggatc cggagcgact gccgggtcga
     1441 ttaccgcatc ctcgaccgcg atcccgccgg acaaacttcg cctgctaccg agcgtcgcga
     1501 gtatttcccg gtcacgcacg tcgagcccga agagggcaac cacgccgtgc tcggcatgga
     1561 tccactgtcc ttcccggtcg cgggcgaggc gatcgaacgc gcgctggcca ccggccagcc
     1621 agcggccagc gcgcgcatgc gcctggtgca ggagcacggg gagcagcatg ccgtggtgct
     1681 ctatcaagcc gttttcgcct cctcgccgcc tgcaccggtc cgcgccccgc tcggagtcgt
     1741 gtccgcggca ctgggcatgg acgacctgct cgcagccgtt tcacgccagg ctgcggcgca
     1801 gggcatcgcg gtgcgactct ccgaccgcga cgccgggccc gagtcggcgc tgcttgccgg
     1861 cgacgatcgc ggacaggagt cggtctggcc ggaagagatc ctggcgcgca ccacgagcat
     1921 cgatttcgcc ggtcgtcgat gggagctgca cacccgcgcc ctgccccgct acatcgaaca
     1981 gctgcgcagc tgggccgcgt ggggcacgat cgcggtcggc gtcctcacca gcgcaatgct
     2041 cggcgcgctc ctgctcatca ccaccggcaa ccgtcgccgc atccaggcgc tggtcgaaca
     2101 ccgcaccgcc gagctccagg ccgcgagcgt acgcttgcat gaaaaccgcg ccgcactgat
     2161 cgacgcccag cgcatcgccc gcctcggcag ctgggagacc ctctgcgcac gcgacgggct
     2221 gcacgcctcg gacgagctcc acctgctgct cgacagcacg gcaggcgacc tgaactcgct
     2281 cgccgacctg atcgacgcga tcgcccccgc cagccgcgcg gccctgaccg acgcgctcga
     2341 gcgcgccgcc acggagccgg gccggatcat gctcgactgc aacaccgaca gcctgccgat
     2401 gcgcacgctg cagttccgca tcgagggcga gcatgtgggc ggccgcctcc ggcgtatccg
     2461 cggtaccgcg caggacgtca ccgcgctgcg cgaggccgaa gcccgcatcc gctacctggc
     2521 tcgcttcgac ccgctcaccg ggctgctcaa ccgcagcgcc tggcaggacc acgcccagga
     2581 cgtgctgcgg cacgccgcgc gccatgacga ccgctgcgcc gtgctgttcc tcgacctcga
     2641 cgacttcaag acggtcaatg actcgctcgg ccacgccgcc ggcgatcgcc tgctcgccca
     2701 ggtggcctgc cgcctcggcg gctgcgtacg cgcggaagac ctggtcgccc gcatcggcgg
     2761 cgacgagttc gtgatcctgt tgtcgcgcct cgcgcacgcc gaagagccgg cgcgcgttgc
     2821 cgagcgcgtg ctcgatgcgc tcgcccgccc gctgcagatc gacagccagc cgctgcgcgt
     2881 cggcgcgagc atcggaatcg ccctctaccc cgccgacggt ggcgagatcg atacgctgct
     2941 caagcacgcc gacactgcga tgtacgaggc caaggagtcc ggccgcaacg cctaccgctt
     3001 cttcctgccc gagatgaccc agcgcgtgac ccgccgactg cagaccgagg ccgagctgcg
     3061 caaggcgatc gagagcggcg atctgcgcct gcactaccag ccccagatcg acgcg
//
LOCUS       DAOQBS010000221         3081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_36365_length_3081_cov_15.346993, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000221 DAOQBS010000000
VERSION     DAOQBS010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36365_length_3081_cov_15.346993"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..495
                     /locus_tag="PKN25_15080"
     CDS             40..495
                     /locus_tag="PKN25_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNV92275.1"
                     /translation="MSYTMPEFLAHAIAMEREAAERYLELADMMEAHNNLEVASLFRD
                     MVRYSNMHGDSIVERAAGHELPRLKSWQYRWVAPTEVGDEDAFDYTMNAWQALEYARE
                     NEARAMNFYRTVSETSTDPEIKTLAMEFATEEADHTMALDKMIAQTQRP"
     gene            754..1065
                     /locus_tag="PKN25_15085"
     CDS             754..1065
                     /locus_tag="PKN25_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNV92276.1"
                     /translation="MKHTAATLALSGLLAFTAVGCAVTRDQSTVGEYVDDATVTTRVK
                     AKFAEDPTVSAMSISVETLKGTVQLSGFAKSTAERNRAVEIARTTPGVKSVKNDIVIR
                     P"
     assembly_gap    1085..1184
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1232..2626
                     /locus_tag="PKN25_15090"
     CDS             1232..2626
                     /locus_tag="PKN25_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNV92277.1"
                     /translation="MNADPEPLPLPPAPSRLPHPRAGRLIAVLLLSFVLGLAWLLAGQ
                     WQSESSRVALEDLRARSVRLHHLDTLLLQMLDAESSVRGYLLTQNPVYLRPYQDGQAA
                     LESTLAALRADTWADERQHETVDHLALLIDMRWSLLTQAIERGVPPGADQPDGGTGKQ
                     VTDDIRTRILALRTATQAEIDATQDGAFARFGDARTMNRVLGASVLALLLVLVVVLYR
                     QDRLRERMAQLLRSENERLHTEVAHRTEELSSLATYLTNAREAEQARLARELHDELGA
                     LLTAAKLDAAWIARKLPADTVAPMRSRFDRLLDTLNQGIALKRKVVAGLRPPLLSELG
                     LLEALRALPDSGDIGEQGGRLAIELPDALPDVPAQTALALYRIAQEALTNVRRYAQAS
                     SVRLRLGVEGDELVLAVEDDGVGFDPAARGHNRHGLAGMAHRVQMLAGRLEIDSAPGK
                     GTRITARVPFARMH"
     gene            complement(2633..3064)
                     /locus_tag="PKN25_15095"
     CDS             complement(2633..3064)
                     /locus_tag="PKN25_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV92278.1"
                     /translation="MSAPLNSESATVPALRVVLVEDSAMLRGMLCEMLAELDGVGVVA
                     EAEDEQGALAALEAQRPDLAIIDLELRMGSGLGVLRQLQAEPERFGRPRAVVFSNYGH
                     APVRARCEQLGVERFFDKSFQLDELIDFVQGAIPGIPGIPG"
BASE COUNT          509 a         1097 c          990 g          385 t
ORIGIN      
        1 tgaagccaaa caaaacgaca cgattccagg aggacgacca tgtcttacac gatgcccgaa
       61 tttctcgccc acgcgatcgc catggagcgc gaggccgccg aacgctacct cgaactggcc
      121 gacatgatgg aggcgcacaa caacctcgag gtcgcctcgc tgttccgcga catggtgcgc
      181 tactccaaca tgcacggcga ctcgatcgtc gagcgcgccg ccggccacga gctgccgcgg
      241 ctcaagtcct ggcaataccg ctgggtcgcg cccaccgagg tcggcgacga ggacgccttc
      301 gactacacca tgaacgcctg gcaggcgctg gaatacgcgc gcgagaacga ggcgcgcgcg
      361 atgaatttct accgcaccgt ctccgagacc agcaccgacc cggagatcaa gacgctggcc
      421 atggagttcg ccaccgagga ggcggaccac acgatggcgc tcgacaagat gatcgcgcag
      481 acgcagcggc cctgagggcg gacgcggacc gtcgccgggc ggtccgccct gcgcaggacg
      541 gcggcctgcg cacgcgcagg aggctcccgc gcgacgcggg cgagcgcccc ggcacgcgat
      601 catcggcatc ccccgacggg aaggtgacgg aatcgtccta cacggttttc cgcctcgtcg
      661 gacaacgccc gcaggaagtc gcgcctatag tggagttgca acccgctcgc gggaccgacc
      721 cgccaagaca cgacaaccct caggagaccc gccatgaaac acaccgccgc caccctcgcc
      781 ctgtccggcc tgctcgcctt caccgccgtc ggctgcgccg tcacccgcga ccaatccacg
      841 gtcggcgaat acgtcgacga cgccaccgtg accacgcgcg tgaaggcgaa gttcgccgaa
      901 gacccgaccg tcagcgccat gtcgatcagc gtggagacgc tcaagggcac ggtgcagttg
      961 tccggcttcg ccaagagcac cgccgagcgc aaccgcgcgg tcgagatcgc gcgcaccacc
     1021 ccgggcgtga agtcggtgaa gaacgacatc gtgatccgtc cttgaggacg cgcgccgcag
     1081 ccccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgtgct gcggacaagc
     1201 ccgcaggcgt gggccaggat aggacaggtt catgaatgcc gatccggagc ctctgccctt
     1261 gccccccgcc ccatcccgcc tgccgcaccc gcgcgcgggg cgcctgatcg ccgtgctgct
     1321 gctgagcttc gtgctcgggc tggcgtggct gctcgccggc cagtggcagt ccgagtcgag
     1381 ccgcgtcgcg ctggaggatc tgcgcgcgcg ctcggtgcgc ctgcatcacc tcgacaccct
     1441 gctgctgcag atgctcgacg ccgagtcgag cgtgcgcggc tacctgctga cgcagaaccc
     1501 ggtctacctg aggccttacc aggacggcca ggccgcgctg gaaagcacgc tcgcagcctt
     1561 gcgcgccgac acctgggcgg acgagcgcca gcacgagacc gtcgaccacc tggcgctgct
     1621 gatcgacatg cgctggagcc tgctcacgca ggcgatcgaa cgcggggttc cgcccggcgc
     1681 cgaccagccc gacggcggca ccggcaagca ggtcaccgac gacatccgca cccgcatcct
     1741 cgccctgcgt accgcaaccc aggccgagat cgacgccacc caggatggcg ccttcgcacg
     1801 ctttggcgac gcgcgcacga tgaaccgggt gctcggcgcg agcgtgctgg cgctgctgct
     1861 ggtgctggtc gtggtgctct accgccagga ccgcctgcgc gaacgcatgg cgcagttgct
     1921 gcgctccgag aacgagcgcc tgcacaccga agtcgcccac cgcaccgagg aactgagcag
     1981 cctggcgacc tacctgacca atgcgcgcga ggccgagcag gcgcgcctcg cgcgcgagct
     2041 gcacgacgaa ctcggcgcct tgctcaccgc cgccaagctc gacgccgcct ggatcgcgcg
     2101 caagcttccg gccgacaccg tggcgccgat gcgcagccgc ttcgaccgcc tgctcgacac
     2161 cttgaaccag ggcatcgcgc tcaagcgcaa ggtggtggcc ggcctgcgtc cgccgctgct
     2221 gtccgagctc ggcctgctcg aagcgctgcg cgccctgccc gacagcggcg acatcggcga
     2281 acagggcggc cggctcgcga tcgagctgcc cgatgccctg cccgatgtgc ccgcgcagac
     2341 ggcacttgcg ctctaccgca tcgcgcagga ggccctgacc aacgtgcgcc gctatgcaca
     2401 ggcgagctcg gtgcgcctgc gcctgggggt cgaaggcgac gagctggtgc tggcggtgga
     2461 agacgacggc gtgggcttcg acccggcggc gcgcggccac aaccgccacg ggctggccgg
     2521 catggcgcac cgggtgcaga tgctcgccgg tcggctggag atcgacagcg cgccgggcaa
     2581 gggcacgcgc atcaccgcac gcgtgccgtt cgcacgcatg cactgaagcg gctcagcccg
     2641 gaatgccggg aatgcctgga atggccccct ggacgaagtc gatcagctcg tcgagctgga
     2701 aggatttgtc gaagaagcgc tcgacgccga gctgctcgca gcgcgcgcgc accggcgcgt
     2761 ggccgtagtt ggagaacacc accgcgcgcg gacggccgaa gcgctccggc tcggcctgca
     2821 gttgcctcag cacgccaagg ccgctgccca tgcgcagctc gaggtcgatg atggcgagat
     2881 ccgggcgctg cgcctcgagc gcggcaagcg ccccttgctc gtcctcggcc tcggccacga
     2941 cgccgacgcc gtcgagctcg gcgagcatct cgcacaacat cccgcgcagc atcgcggagt
     3001 cttccacgag cacgacgcgc agcgcgggga cggtggcaga ttcggaattg agtggcgcgc
     3061 tcatcgacac gagctcctca g
//
LOCUS       DAOQBS010000222         3061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_36752_length_3061_cov_13.225216, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000222 DAOQBS010000000
VERSION     DAOQBS010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36752_length_3061_cov_13.225216"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /locus_tag="PKN25_15100"
     CDS             complement(<1..1709)
                     /locus_tag="PKN25_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC domain/YcaO domain-containing protein"
                     /protein_id="HNV92279.1"
                     /translation="MEIKVNFLDKLRLEARFDDFTVIADQPIRYKGDGSAPGPFDYFL
                     ASSALCAAYFVKLYCDTRNLPTDNIRLSQNNIVDPENRYKQIFKIQVELPEDLSAKDR
                     QGILRSIERCTVKKVVQTGPEFVIEEVENLDADAQALLTLNPDPDAHTYILGKDLPLE
                     QTIANMSKLLADLGIKIEIASWRNLVPNVWSLHIRDAHSPMCFTNGKGASKESALASA
                     LGEYIERLNCNHFYNDQFWGEDIADAAFVHYPNERWFKPGRKDALPAGLLDEYCLEIY
                     DPEGELRASHLYDTNSGNVARGICALPYVRQSDGEVVYFPTNLIDNLYLSNGMSAGNT
                     LPEAQVQCLSEIFERAVKREIIEGEMALPDVPPEVLAKYPGVLAGIEALEAQGFPVLV
                     KDASLGGKYPVMCVTLMNPRTGGVFASFGAHPSLEVALERSLTELLQGRSFEGLNDLP
                     APTFESNAVTEPNNFVEHFIDSSGVVSWRFFSAKADYPFVEWDFSSHGERSNAEEAAT
                     LFGILEDMGKEVYMAVFDQLGATVCRIVVPGYSEVYPVDDLIWNNTNKALAFRADILN
                     LHRL"
     assembly_gap    1888..1987
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1988..2465)
                     /locus_tag="PKN25_15105"
     CDS             complement(<1988..2465)
                     /locus_tag="PKN25_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92280.1"
                     /translation="MSRLSTESVAKVVDAVQAMDRAQKERLADEIFRMQPNMLGSILV
                     LPRLGVPMEKVEFAVGVLLVCFQAMKESGLAWPVITEDEQERQLARFVAATRLGEDIA
                     PSLRSRALRRYIAAHPEKGLAAYVASDTSKWLGEVAADESDKYAMLAVWNLVNCIAF"
     gene            complement(2462..2680)
                     /locus_tag="PKN25_15110"
     CDS             complement(2462..2680)
                     /locus_tag="PKN25_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92281.1"
                     /translation="MRFKVYFDDETAQRLTAAPKAMTEKFAAITALTDAFCAQPLDAE
                     LRRMILLVVRACGGAGERFACGSRGGFE"
BASE COUNT          496 a          975 c          971 g          519 t
ORIGIN      
        1 aggcgatgca ggttgaggat gtcggcgcgg aaggccagcg ccttgttggt gttgttccag
       61 atcaggtcat ccaccggata gacctcggaa taccccggca cgacgatgcg gcagacggtg
      121 gcgccgagct ggtcgaacac cgccatgtac acttccttgc ccatgtcctc gaggatgccg
      181 aacagggtcg cggcttcctc ggcgttggac ctttcaccgt gggaagagaa atcccattcg
      241 acgaacgggt aatcggcctt ggcgctgaag aagcgccacg acaccacgcc gctcgaatcg
      301 atgaagtgct cgacgaaatt gttcggctcg gtgaccgcgt tgctctcgaa ggtgggcgcg
      361 ggcaggtcgt tgaggccctc gaagctgcgc ccctgcagca gctcggtcag gctgcgctcc
      421 agcgccacct ccaggctcgg gtgcgccccg aacgaggcga acacgccgcc cgtgcgcggg
      481 ttcatcaggg tcacgcacat caccggatac ttgccgccca gcgacgcatc cttcaccagc
      541 accggaaagc cctgcgcctc cagcgcctcg atgccggcga gcacgccggg atacttcgcc
      601 agcacctctg gcggcacatc gggcagcgcc atctcgcctt cgatgatctc gcgcttgacc
      661 gcgcgctcga agatctccga caggcactgc acctgcgcct cgggcagcgt gttgccggcg
      721 ctcatgccgt tgctgaggta gaggttgtcg atcaggttgg tggggaaata caccacctcg
      781 ccgtccgact ggcgcacgta gggcagcgcg cagatgccgc gcgccacgtt gccggagttg
      841 gtgtcgtaca gatgcgaggc gcgcagttcg ccctcggggt cgtagatctc caggcaatac
      901 tcgtcgagca gcccggccgg cagcgcatcc ttgcggccgg gcttgaacca gcgctcgttg
      961 gggtaatgca cgaacgccgc gtcggcgatg tcctcgcccc agaactggtc gttgtagaag
     1021 tggttgcagt tgaggcgctc gatgtattcg cccagcgccg acgccaaggc gctctccttg
     1081 ctcgccccct tgccgttggt gaagcacatc ggcgaatgcg catcgcggat gtgcagcgac
     1141 cacacgttgg gcacgaggtt gcgccacgac gcgatctcga tcttgatgcc gaggtccgcc
     1201 agcagcttcg acatgttggc gatcgtctgc tccagcggca gatccttgcc gaggatgtag
     1261 gtgtgggcgt ccggatccgg attcagcgtc agcagggcct gcgcatcggc gtccaggttc
     1321 tccacctcct cgatgacgaa ctccggcccg gtctgcacca ccttcttcac ggtacagcgc
     1381 tcgatcgagc gcaggatgcc ctggcggtcc ttggcggaga ggtcctccgg cagctcgacc
     1441 tggatcttga agatctgctt gtagcggttc tcggggtcga cgatgttgtt ctgcgacagg
     1501 cggatgttgt cggtggggag gttgcgggtg tcgcagtaca gcttcacgaa gtaggccgcg
     1561 cacaaggccg acgaggccag gaaatagtcg aacggccccg gcgccgagcc gtcgcccttg
     1621 tagcggatcg gctggtcggc gatcaccgtg aagtcgtcga acctggcttc gagacgaagc
     1681 ttgtcgagga agttgacctt gatttccatg agggggaagg aatcctgaaa gggcgtgaac
     1741 acgcgtcggg cggcattatc gccggtttcg cgggggcgga cggggttcgg ggcgggctgc
     1801 ggctcctgca ggagcgccgc aaggcgcgaa cgcggccgcg ccgagacgcc tacgtgggct
     1861 tcgtgcgctt cgactgtggc agctcgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnncga aggcgatgca gttcaccagg ttccacaccg ccagcatcgc gtacttgtcg
     2041 gactcgtcgg ccgcgacctc gcccagccac ttcgacgtat cgctcgccac gtaggctgcc
     2101 agccccttct cggggtgcgc ggcgatatag cgccgaagcg cgcgactgcg cagcgacggg
     2161 gcgatgtcct cgccgaggcg cgtcgccgcc acgaagcgcg cgagctggcg ctcctgctcg
     2221 tcctccgtga tgaccggcca ggccagcccg gactccttca tcgcctggaa gcagaccagc
     2281 agcaccccga cggcgaactc gaccttctcc atcggcaccc cgagccgggg cagcaccagg
     2341 atcgagccca gcatgttcgg ctgcatgcgg aagatctcgt cggcgagacg ctctttctgc
     2401 gcgcggtcca tcgcctgaac ggcatcgacg accttcgcca ccgactcggt cgacaaccgg
     2461 ctcattcgaa acctccgcgc gatccgcacg caaaccgctc acccgcccca ccgcatgcgc
     2521 ggacgacgag gaggatcatc cgccggagtt cagcgtcgag aggctgcgcg cagaacgcgt
     2581 cggtcagcgc ggtgatagcg gcaaacttct ccgtcatggc cttgggtgcc gccgtcagcc
     2641 gctgcgccgt ctcgtcatcg aagtagacct tgaaacgcat cgacgcctcc gggtgcttcg
     2701 tattcgtggc cacccgtgcg ccgtaaaaca gccccggaga gcgagtttct gagcccgaaa
     2761 caggccaccc gccttaaagc cgccggctcg ccaatcggcg cgaacgccct ctggaccgcc
     2821 tgccacgcac tggccaaaga cgccagcctg ttcacgcacg acacccgcga gttcgaacgt
     2881 gtcgcagggc tgcgggtaga cgactgggtt cgtgcgatgg acgcccgatt tgcgtgggtg
     2941 gcggcgtagc gtactcgtcg gcacccttag ctggcgcaac ggcaatcgtc gttcgagcgg
     3001 agcggctcgt ggtcggcctg ctccgtcctt cgattctgct cggttgcatg cggaggggat
     3061 g
//
LOCUS       DAOQBS010000223         3028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_37415_length_3028_cov_15.667676, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000223 DAOQBS010000000
VERSION     DAOQBS010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37415_length_3028_cov_15.667676"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PKN25_15115"
     CDS             complement(<1..961)
                     /locus_tag="PKN25_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNV92282.1"
                     /translation="MGFELPYGFARDHGVLVAALGAEHAEVLVREDVRPQALAEVRRS
                     VGLPLRLQRVPRALFDARLAEAYGGAGDSAAEIVADVGQEVDLDRLMTELPAVEDLLD
                     TQDDAPIIRLINALLTQAVRDGASDVHIEPYERESVVRLRRDGVLRDIARPRRGLHAA
                     MASRIKIMAQLDIAEKRLPQDGRITLRLAGRAVDVRVSTVPTTHGERLVLRLLDKQAG
                     QIGLDGLGMAPDTSAVFADLLARPHGILLVTGPTGSGKTTTLYAALQSLDAGRLNIVT
                     VEDPVEFDLPGVGQIPVNPRIELDFARALRAILRQDPDVIMIGE"
     gene            complement(961..3027)
                     /gene="gspD"
                     /locus_tag="PKN25_15120"
     CDS             complement(961..3027)
                     /gene="gspD"
                     /locus_tag="PKN25_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HNV92283.1"
                     /translation="MHAAGEPVALNFQNAEIDAVIQAIGRISGRNFLIDPRVKGKINL
                     VTNTPVAPELTYQILLSALRLQGYAAIEESGVTKLVPEADAKLHGVPVVSGRPGAGAA
                     RGDRLVTQVFALRHESAVHMVNVVRPLVSANNAVTAFPGNNTLVVTDYAENLRRLATV
                     IDSIDTPQGDVAVLQLQHAMANDLAPVLVRLLAEQQTGGQNAGGLAPPQIVAEPNSNS
                     LLVRADTPSRLAALRQLVTALDRPGAGGDIRVVYLRNADAVQVAATLNAALAGEGGTS
                     DAASTQPLSRNSGGGSGFGTGSSAPSGMGVASAPLRGTGSTAPSAAGGLSSATRGGMV
                     QPDAVNNALIITAPDAVYRNLRAVIDQLDRRRAQVYIEALIAEISTERAAEFGFQWVA
                     GASAGEVGVLGAQLFNSGGGNLGQLIAGSADRTRLTLPNNGLNLVVGGGKVNVPGIGE
                     VLSLGVLARFLENEVSANILSTPNLITLDNEEARIVVGRNLPFVTGQYANTGGGTTPA
                     NPFQTIERRDVGLTLHVKPQISEGGAIRLQIYQEASAVVGSAESAAAGNSVTTKRSIE
                     SMVLVDDGAILALGGLVEDSYSGGEEKVPVLGDLPFAGSLFRYDTRKRAKTNLVVFLR
                     PVILRDRESYAALTDSRYDYVIGRRDQLPSGANLLRDPPPGEPAPVVDLAFRPLQPAA
                     TPAAPR"
BASE COUNT          396 a         1012 c         1170 g          450 t
ORIGIN      
        1 tctcgccgat catgatcacg tccgggtcct ggcgcaggat ggcgcgcagc gcgcgcgcga
       61 agtcgagctc gatgcgcggg ttgaccggga tctggccgac cccgggcaga tcgaattcca
      121 ccggatcctc gaccgtgacg atgttgaggc ggccggcatc caggctctgc agcgccgcgt
      181 acagcgtggt cgtcttgccc gagccggtgg ggccggtgac gagcaggatg ccgtgcggcc
      241 gggcgagcag gtcggcgaac acggcgctgg tgtcgggcgc catgccgagg ccgtcgaggc
      301 cgatctgccc ggcctgcttg tcgagcaggc gcagcaccag gcgttcgccg tgggtggtcg
      361 gtacggtgga cacgcgcaca tcgaccgccc gcccggccag gcgcagggtg atgcggccgt
      421 cctggggcag gcgtttctcg gcgatgtcga gctgcgccat gatcttgatg cgcgaggcca
      481 tcgccgcgtg caggccgcgg cgcggtcggg cgatgtcgcg cagcacgccg tcgcggcgca
      541 ggcgcaccac cgactcgcgc tcgtagggct cgatgtgcac gtccgaggcg ccgtcgcgca
      601 ccgcctgggt gagcaaggcg ttgatcaggc ggatgatcgg cgcgtcgtcc tgggtgtcga
      661 gcaggtcctc gaccgcgggc agctcggtca tcaggcgatc gaggtcgacc tcctgaccga
      721 cgtcggcgac gatctccgcc gcactgtcgc cggcgccgcc gtaggcctcg gcgaggcggg
      781 cgtcgaagag cgcgcgcggc acgcgctgca ggcgcagtgg caggccgacg ctgcggcgca
      841 cttcggcgag cgcttgcggg cgaacgtcct cgcgcaccag cacctcggcg tgttcggcgc
      901 cgagggccgc gaccagcacg ccgtggtcgc gcgcgaagcc gtagggcagc tcgaaaccca
      961 tcagcgcggg gccgccggcg tcgcggcggg ctgcaagggg cggaaggcga ggtcgaccac
     1021 cggcgccggc tcgcccggcg gcgggtcgcg cagcagattg gcgcccgagg gcagctggtc
     1081 gcggcggccg atcacgtagt cgtagcggct gtcggtgagc gcggcgtagc tttcgcggtc
     1141 gcgcaggatc accgggcgca ggaacaccac caggttggtc ttggcgcgct tgcgcgtgtc
     1201 gtagcggaac aggctgccgg cgaagggcag gtcgccgagc accggcacct tctcctcgcc
     1261 gccgctgtag ctgtcctcga ccagcccgcc gagggcgagg atggcaccgt cgtcgaccag
     1321 caccatggac tcgatcgagc gcttcgtggt caccgagttg ccggcggcgg cgctctcggc
     1381 gctgcccacc acggccgagg cctcctggta gatctgcagg cggatcgcgc cgccctcgga
     1441 gatctgcggc ttgacgtgca gggtgaggcc gacatcgcgg cgctcgatgg tctggaaggg
     1501 gttggcgggc gtggtgccgc cgccggtgtt ggcgtactgg ccggtgacga agggcaggtt
     1561 gcgaccgacg acgatgcgcg cctcctcgtt gtcgagggtg atgaggttgg gcgtggacag
     1621 gatgttggcg ctgacctcgt tctcgaggaa acgggccagc acgcccaggc tgagcacctc
     1681 gccgatgccg ggcacgttga ccttgccgcc gcccaccacc aggttgaggc cattgttcgg
     1741 cagcgtcagc ctggtccggt cggcgctgcc ggcgatgagc tggccgaggt tgccgccgcc
     1801 gctgttgaag agctgcgcgc cgagtacgcc gacctcgccc gccgaggcgc cggcgaccca
     1861 ctggaagccg aactcggcgg cgcgttcggt ggagatctcg gcgatcagcg cctcgatgta
     1921 gacctgggcg cggcggcggt cgagctggtc gatgaccgcg cgcaggttgc ggtagacggc
     1981 gtcgggcgcg gtgatgatca gcgcgttgtt caccgcgtcc ggctgcacca tgccgccgcg
     2041 cgtggcggag gaaagaccgc ctgctgcgct gggggcggtg gagccggtgc cgcgcagggg
     2101 ggcgctggcg acgcccattc cggacggggc ggaactgcct gtgccgaagc cactgccgcc
     2161 gccgctgttg cgcgacagcg gctgcgtgct tgcagcgtcg ctcgtgccgc cctcgccggc
     2221 gagcgcggca ttgagcgtcg cggcgacctg gacggcgtcg gcgttgcgca gatacaccac
     2281 gcgaatgtcg ccgcccgcgc ccggacggtc gagcgcggtg acgagctggc gcagcgcggc
     2341 caggcggctc ggcgtgtcgg cacgcaccag caggctgttg ctgttgggtt cggcgacgat
     2401 ctgcggcggt gcgagcccgc ccgcgttctg gccaccggtc tgctgctcgg cgagcaggcg
     2461 gacgagcacc ggggcaaggt cgttggccat cgcgtgctgc agctgcagca ccgcgacgtc
     2521 gccctgcggc gtgtcgatcg agtcgatcac ggtcgccagc cggcgcaggt tctcggcgta
     2581 gtcggtgacc accagggtgt tgttgccggg gaaggcggtg accgcgttgt tggccgagac
     2641 cagcgggcgc accacgttga ccatatgcac cgcggattca tggcgcagcg cgaacacctg
     2701 ggtcaccagg cggtcgccgc gcgctgcgcc cgcgcccgga cggccactga ccaccggcac
     2761 gccgtgcagc ttggcgtcgg cctcgggcac gagcttggtc acgcccgact cctcgatcgc
     2821 cgcgtagccc tgcaggcgca gcgccgacag caggatctgg taggtcagct ccggggccac
     2881 gggggtgttg gtgacgaggt tgatcttgcc cttgacgcgt ggatcgatga ggaagttgcg
     2941 ccccgagatg cgtccgatcg cctggatcac cgcgtcgatc tcggcgttct ggaagttgag
     3001 cgccaccggc tcgcccgcgg cgtgcacg
//
LOCUS       DAOQBS010000224         2983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_38254_length_2983_cov_16.767418, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000224 DAOQBS010000000
VERSION     DAOQBS010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38254_length_2983_cov_16.767418"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PKN25_15125"
     CDS             complement(<1..892)
                     /locus_tag="PKN25_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNV92284.1"
                     /translation="MQEATLSFKARAERALADPTLKIAIDRTTGTAERKRALAMSQFP
                     QFEAARERGKRIKDHVIANLDHYLLEFERNAIASGAKVYWAATAEEASQIVVRLCKEA
                     GAKRVTRVKSMLGEEIGLPHALDEAGIERVETDLAEHIVQLGGDRPSHIVWPSMHRTR
                     EQVSEMFKRLHRAPHQEETIEAMVDSARRELRDKFLSADVAISGANFLLADTGATCTV
                     TNEGNAELTTTPPRVHIVTAGIEKLVPSMGHAIAMLRLLVRSATGADVTQYTTFHCGP
                     KQAGDRDGPEEFHIVLVDNGR"
     gene            complement(896..1705)
                     /locus_tag="PKN25_15130"
     CDS             complement(896..1705)
                     /locus_tag="PKN25_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNV92285.1"
                     /translation="MNAPPSVSRSDSRSDSRPVSPRVALFATCLVDAMRPSVGFAALK
                     LLRDAGCRVEIPQAQTCCGQPAFNSGDTAHAATLARHFIETFEGYDYVVAPSGSCIGM
                     TKVHYAEALADDPAWAERARRLGERSFELMSFLVDVMGYAPPDVKLDTSFTYHDSCSG
                     LRELGVHDQPRALLGQVAGLTLKPLEGNDVCCGFGGTFCVKYAAISNAVVGEKAEAIE
                     RSGAQLLLGGDLGCLMNMAGKLKRKGSSVRAFHAAEVLAGMGGGPAIGEEG"
     gene            1994..2905
                     /gene="fdhE"
                     /locus_tag="PKN25_15135"
     CDS             1994..2905
                     /gene="fdhE"
                     /locus_tag="PKN25_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HNV92286.1"
                     /translation="MNTQAIELPPSGEAAAVLLPDPGTVFLARAERFDALALHHGLGE
                     WLAFLGRLSHAQHAALQASAQDITPPAEDVLERAAKHRMPPISVASYQRPAVWREILR
                     NLVGDLLENAPVGARETLDGLLVTSAEELDKLGEKLLNGEPEGADMARLPYVAAAMQV
                     VFTALASKLDAGALPLMEAHSTCPVCGSHPVVSVIRSDGPTAGLRYVHCTLCNTEWHV
                     PHATCVSCGDRDKITLHEIDGDDGIARAESCGSCESYTKLIVQPKNVRVDPIADDLAS
                     VALDMLVDDAGFVRSGPNYLLIGAGES"
BASE COUNT          432 a         1029 c         1059 g          463 t
ORIGIN      
        1 tgcggccgtt gtcgaccagc acgatgtgga attcttccgg cccgtcgcga tcgcccgcct
       61 gcttcggccc gcagtggaag gtggtgtatt gggtgacgtc cgcgccggtg gccgagcgca
      121 ccagcaggcg cagcatggcg atcgcgtggc ccatgctggg gacgagcttc tcgatgcccg
      181 cggtgacgat atgcacccgc ggcggcgtgg tggtgagctc ggcgttgccc tcgttggtga
      241 cggtgcaggt ggcgccggtg tcggcgagca ggaagttggc gcccgagatg gcgacgtcgg
      301 ccgacaggaa cttgtcgcgc agctcgcgcc gggcgctgtc gaccatggcc tcgatggtct
      361 cctcctggtg gggcgcgcgg tgcaggcgct tgaacatttc cgagacctgc tcgcgcgtgc
      421 ggtgcatcga cggccacacg atgtgcgagg ggcgatcgcc acccaactgc acgatgtgct
      481 cggcgaggtc ggtctcgacg cgctcgatgc cggcttcatc cagggcgtgc ggcaggccga
      541 tctcctcgcc cagcatcgac ttgacgcgcg tcacgcgctt cgcgcccgcc tccttgcaca
      601 ggcgcacgac gatctgcgag gcctcctcgg cggtcgcggc ccagtacacc ttggcgcccg
      661 aggcaatggc gttgcgctcg aactcgagca ggtagtggtc gaggttggcg atcacgtgat
      721 ccttgatgcg cttgccgcgc tcgcgcgccg cctcgaactg cgggaactgg ctcatcgcca
      781 gcgcgcgctt gcgctcggcg gtgccggtgg tacggtcgat ggcgatcttg agcgtggggt
      841 cggcgagcgc acgctcggcg cgcgccttga aggagagcgt ggcctcttgc atgactcagc
      901 cctcctcgcc gatggccgga ccgccgccca tgccggcgag cacctcggcg gcgtggaagg
      961 cgcgcacgct cgagcccttg cgcttgagct tgcccgccat gttcatcagg cagccaaggt
     1021 caccgccgag cagcagctgc gcgccgctgc gctcgatggc ctcggccttc tcgccgacga
     1081 ccgcgttgga gatcgccgcg tacttgacac agaaggtgcc gccgaagccg cagcacacgt
     1141 cgttgccttc gagcggcttc agggtgagcc cggcgacctg accgagcagc gcacgcggct
     1201 ggtcgtgcac gccgagctcg cgcaggcccg agcagctgtc gtggtaggtg aagctggtgt
     1261 cgagcttcac gtcggggggg gcgtagccca tcacgtcgac caggaagctc atcagctcga
     1321 agctgcgctc gcccaggcgg cgtgcgcgct cggcccaggc cgggtcgtcg gcgagcgcct
     1381 cggcgtagtg caccttggtc atgccgatac aggagcccga gggcgcgacc acgtagtcgt
     1441 agccctcgaa ggtctcgatg aaatggcgtg ccagcgtggc cgcgtgcgcg gtatcgccgc
     1501 tgttgaaggc gggctggccg cagcaggtct gcgcttgcgg gatctcgacc cggcagccgg
     1561 cgtcgcgcag cagcttgagc gcggcgaagc cgacgctggg gcgcatcgcg tccaccaggc
     1621 aggtggcgaa cagcgcgacg cgcggcgaga ccgggcgcga atcggagcgc gaatcggagc
     1681 gcgagacgct tgggggtgcg ttcatggggg tcttcctcca ggttgtccgg gcgatcgtcc
     1741 gcgggcgttc ggcgcaccgc aaaggggcca ggccccggca gcaccgcctc ctcgccacgt
     1801 cggacagaat acgattgttt ttcgtggtgc ggcgcggatt cgggaccggt ctacgggcaa
     1861 accctgaagg gccccggcgt cacgcctgca aggccgtcgc gacctccagc ggagggtggt
     1921 ttctgcatgt ccggcttgca ttcggcacgc tactgcgccg atcatcgcgt gcgcttttcc
     1981 atccagagac aacatgaaca cccaagccat cgaactgccg ccctcgggcg aagccgccgc
     2041 cgtcctcctg cccgatccgg gcaccgtctt cctcgcgcgt gccgagcgtt tcgacgccct
     2101 cgcgctgcac cacggcctgg gcgagtggct cgccttcctc ggcaggctgt cgcatgccca
     2161 gcatgcggcg ctgcaagcct cggcacagga catcaccccc ccggcggaag acgtcctcga
     2221 acgcgcagcc aagcaccgca tgccgccgat cagcgtggcc tcgtaccagc gccccgcggt
     2281 gtggcgcgag atcctgcgca acctcgtcgg cgacctgctg gagaacgcgc cagtgggcgc
     2341 gcgcgagacg ctcgacgggc tgctcgtcac cagcgccgag gagctcgaca agctgggcga
     2401 gaagctgctc aacggcgaac ccgagggcgc cgacatggcc cgtctgccct acgtcgcggc
     2461 ggcgatgcag gtggtgttca ccgcgctcgc cagcaagctc gacgccggcg cgctgccgct
     2521 gatggaagcc cacagcacct gcccggtgtg cggcagccac ccggtggtca gcgtgatccg
     2581 cagcgacggc ccgaccgccg gcctgcgcta cgtgcattgc acgctgtgca acaccgagtg
     2641 gcacgtcccg cacgccacct gcgtgagctg cggcgaccgc gacaagatca ccctgcacga
     2701 gatcgacggc gacgacggca tcgcgcgcgc ggagtcctgc gggtcctgcg agagctacac
     2761 caagctcatc gtccagccga agaacgtgcg cgtcgacccg atcgccgacg acctcgcctc
     2821 ggtggcgctg gacatgctgg tcgacgacgc gggtttcgtc cgcagcgggc cgaattacct
     2881 gctcatcggc gccggggagt cgtaacaacg ccaaggcagt gcgggggcgg cggcgcgggc
     2941 ttcgggcgtg gggatcgcga cttgcgtcgc tcctaccgct ggg
//
LOCUS       DAOQBS010000225         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_38905_length_2950_cov_16.424180, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000225 DAOQBS010000000
VERSION     DAOQBS010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38905_length_2950_cov_16.424180"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /gene="ftsY"
                     /locus_tag="PKN25_15140"
     CDS             <1..969
                     /gene="ftsY"
                     /locus_tag="PKN25_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNV92287.1"
                     /translation="AAAAVAKKSWTERLKAGLARTRNQIGGGLASLFGLRKIDEDLLE
                     ELESTLLMADCGVDATQHLIDDLRRRWKRDRLETADQLQKALADGLHEIIAPLEAPLE
                     VAGHRPFIIMIAGVNGSGKTTSIGKLAKYFQAHGKSVLLAAGDTFRAAAREQLMTWGE
                     RNNVTVVAQDSGDAAAVIFDAINAARARKIDVVLADTAGRLPTQLHLMEEIAKVRRVI
                     AKADPSGPHEVLLVLDANIGQNALAQVKAFDKAIGVTGLVVTKLDGTAKGGVLAAIAR
                     QCPKPLRFIGVGEGIDDLQPFKAREFVDALFEPGAGAVKAGAEARR"
     gene            966..1664
                     /locus_tag="PKN25_15145"
     CDS             966..1664
                     /locus_tag="PKN25_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV92288.1"
                     /translation="MIVFEDVAKRYAGGYTALAGVSFEIRHGELVVLSGHSGAGKSTL
                     FKLIPVIERPTAGRVLINGQDVSHLPRTAIPYLRRNLGLVLQESRLLFDRNVYDNVML
                     PLVITGHPAADAAKRVAAALERVGLDGRGKEMPAGLSGGEQQRVAIARAIVNRPSILI
                     ADEPTAHLDPAYAADIAALFRSFNQAGVTVLVSTHDASLFAASRPRRLVLAKGQLVED
                     SRPGGRPAQEEVAA"
     gene            1661..2557
                     /gene="ftsX"
                     /locus_tag="PKN25_15150"
     CDS             1661..2557
                     /gene="ftsX"
                     /locus_tag="PKN25_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNV92289.1"
                     /translation="MINWFYLHLRAIRHALHRLVSQPVGTLLSALVVGIALSLPGGGY
                     LLLDNLGSLARGVSGTPEISLFLDMNAGAAEVAAIEQRLKGESELASYRFVPRDEGLR
                     QLEAAGLGDVLGGLKANPLPDAFVLAPRREDPALFERMAERMRSWPKVAHVQLDSAWV
                     ERLHALLGLGRSAVLMLAGLLGFALVVVTFNTIRLQILTQRQEIAVSRLLGATDPFIR
                     RPFYWFGGLQGALGGLVALGTMALGIEALAAPVARLAETYGAVFALSGPEPEASLAIV
                     AFAAFLGWLGAAISVRRHLAGT"
     gene            2790..>2950
                     /locus_tag="PKN25_15155"
     CDS             2790..>2950
                     /locus_tag="PKN25_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNV92290.1"
                     /translation="MSLINTQVQPFKAQAFHNGKFVEVSDASMKGKWSVVIFMPAAFT
                     FNCPTEIEDA"
BASE COUNT          447 a         1060 c          987 g          456 t
ORIGIN      
        1 gccgccgccg cggtcgcgaa gaagtcgtgg accgagcgcc tcaaggccgg cctcgcgcgc
       61 actcggaacc agatcggcgg agggctggcg agcctgttcg gcctgcgcaa gatcgacgag
      121 gacctgctcg aggagctcga atccaccctg ctgatggccg actgcggcgt cgacgccacc
      181 cagcacctga tcgacgacct gcgccggcgc tggaagcgcg accgcctgga gaccgccgac
      241 cagctgcaga aggcgctcgc cgacggcctg cacgagatca tcgcgccgct cgaagcgccg
      301 ctcgaggtcg caggtcatcg ccccttcatc atcatgatcg cgggcgtcaa cggctccggg
      361 aagacgacct cgatcggcaa gctggccaag tacttccagg cccatggcaa gagcgtgctg
      421 ctcgccgcgg gcgacacctt ccgcgccgcc gcgcgcgagc agctgatgac ctggggcgag
      481 cgcaacaacg tcaccgtggt ggcgcaggac agcggcgacg ccgcggcggt gatcttcgac
      541 gccatcaacg ccgcacgtgc gcgcaagatc gacgtcgtgc tcgccgacac cgccggccgc
      601 ctgcccaccc agctccacct catggaagag atcgccaagg tgcgccgggt gatcgccaag
      661 gccgacccga gcgggccgca cgaggtgctg ctggtgctcg acgccaacat cggccagaac
      721 gcgctcgccc aggtgaaggc cttcgacaag gcgatcggcg tcaccggcct ggtggtcacc
      781 aagctcgacg gcaccgccaa gggcggcgtg ctggcggcga tcgcgcgcca gtgccccaag
      841 ccgctgcgct tcatcggcgt cggcgagggc atcgacgacc tgcagccgtt caaggcgcgc
      901 gagttcgtcg atgcgctctt cgagccgggt gccggtgcgg tcaaggcggg agcggaagcg
      961 cgcagatgat cgtcttcgag gacgtggcca agcgctatgc gggcggctac acggcgctgg
     1021 cgggcgtcag cttcgagatc cgccatggcg agctggtcgt gctctccggc cattccggcg
     1081 cgggcaagag cacgctgttc aagctcatcc cggtcatcga gcgcccgacc gccggtcgtg
     1141 tgctgatcaa cggtcaggac gtgtcgcacc tgccgcgcac ggccatcccc tacctgcgcc
     1201 gcaacctcgg cctggtgctg caggagagcc ggctgctgtt cgaccgcaac gtctacgaca
     1261 acgtgatgct gccgctggtg atcaccggcc accccgcggc cgacgccgcg aagcgggtgg
     1321 cggccgcgct cgagcgcgtg ggcctggacg ggcgtggcaa ggagatgccg gcgggtctgt
     1381 cgggcggcga gcagcagcgc gtggcgatcg cgcgcgcgat cgtcaaccgg ccgtcgatcc
     1441 tcatcgccga cgagcccacc gcccacctcg accccgccta cgccgccgac atcgcggcac
     1501 tgttccgctc cttcaaccag gccggcgtca ccgtgctcgt gtccacccac gacgccagcc
     1561 tgttcgccgc cagccggccg cgccggctcg tgctggccaa ggggcagctg gtcgaggact
     1621 cgcgcccggg cggacggccg gcgcaggagg aggttgcggc gtgatcaact ggttctacct
     1681 gcacctgcgt gcgatccgtc acgcactgca ccggctggtg tcgcagcccg taggcaccct
     1741 gctgtcggca ctggtggtcg gcatcgcgct gtcgctgccg ggcggcggct acctgctcct
     1801 cgacaacctc ggctcgctgg cgcgcggcgt ctccggcacg cccgagatca gcctgttcct
     1861 ggacatgaac gccggcgcgg ccgaggtcgc cgcgatcgag cagcgcctga aaggcgagag
     1921 cgaactcgcc agctaccgct tcgtgccacg cgacgagggc ttgcgccagc tcgaggctgc
     1981 aggcctgggc gacgtgctcg gcgggctcaa ggccaacccg ctgcccgacg ccttcgtgct
     2041 cgcgccgcgc cgcgaggatc cggcgctgtt cgagcgcatg gccgagcgca tgcgctcctg
     2101 gcccaaggtg gcgcacgtgc agctcgactc ggcctgggtc gagcgcctgc atgcgctgct
     2161 cgggcttgga cgttcggcgg tgctgatgct cgccggcctg ctcggcttcg cgctggtggt
     2221 ggtcaccttc aacaccattc gcctgcagat cctcacccag cgccaggaga tcgcggtgag
     2281 ccggctgctc ggcgccaccg atcccttcat ccgccgcccc ttctactggt tcggcggcct
     2341 gcagggcgcg ctcggcggtc tcgtcgcgct cggcaccatg gcgctcggca tcgaggcgct
     2401 cgccgccccg gtggcgcggc tcgccgagac ctacggcgcc gtcttcgcgt tgagcgggcc
     2461 cgagcccgag gccagcctgg cgatcgtcgc cttcgcggcc ttcctcggct ggctgggcgc
     2521 ggcgatctcg gtgcggcggc atctcgccgg cacttgaact ttcgtcatcc atccccagtt
     2581 acattcgatc gtcgtttgca cgctgcccgc ggacgttgtc ccgggcggtg gcggcgcgat
     2641 cgacgatgca ctccggtaat cgcgagtcca tagggacctc caatcgactg gatctccata
     2701 atcaattaga gcaattcaag gaaccggtct aaactttgct ccatcgagtt gcaccgaacc
     2761 gttccaacaa cccttctgga ggattacaga tgtcgctgat caacacccag gttcaaccgt
     2821 tcaaagccca ggccttccac aacggcaagt tcgtcgaagt ctccgatgcg agcatgaagg
     2881 gcaagtggtc cgtggtgatc ttcatgccgg ccgccttcac cttcaactgc ccgaccgaga
     2941 tcgaggacgc
//
LOCUS       DAOQBS010000226         2901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_39968_length_2901_cov_12.902670, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000226 DAOQBS010000000
VERSION     DAOQBS010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39968_length_2901_cov_12.902670"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PKN25_15160"
     CDS             <1..1065
                     /locus_tag="PKN25_15160"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNV92291.1"
                     /translation="LREIGIQPDILLCRADRPVPEDERRKIALFCNVMPEAVIECLDA
                     DSIYKIPGQLHDQMLDQIVCHKLDILARAADLSVWDRLIHALENPKQTVDIGFVGKYV
                     DLTESYKSLIESLNHAGMHTESKVRIHYIDSEDIERDGCDVLESMDAILVPGGFGKRG
                     TEGKIAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMEGAHSTEFERDTRFPVIGL
                     ITEWKDRTGKIEKRSEESDLGGTMRLGGQLCLLKEGTLARSIYGAPEVMERHRHRYEV
                     NNTLLARLEDKGLVVSGRAPVTDLCEMVELPADVHPWFVGCQFHPEFTSNPRKGHPLF
                     TAYVKAAIAHKQAAAPRSEA"
     gene            1062..1901
                     /gene="kdsA"
                     /locus_tag="PKN25_15165"
     CDS             1062..1901
                     /gene="kdsA"
                     /locus_tag="PKN25_15165"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNV92292.1"
                     /translation="MNLCGFEVGLDKPVFLISGPCVAESEQMCLDIAGQMKEICAELG
                     IPYIFKASYDKANRSSGKSFRGHGMDAGLKMLEAVKKQLGLPVLTDVHTIEEIPTVAA
                     VVDVLQTPAFLCRQTDFIHAVAASGKPVNIKKGQFLAPGDMKNVADKAREANGGADTI
                     MVCERGASFGYNNLVSDMRALAIMRDTGCPVVFDATHSVQLPGGQGTASGGQREFVPV
                     LARAAVAVGVAGLFMETHPDPAKALSDGPNAWPLPKMKALLTVLKDIDALVKRQGFLE
                     TTL"
     gene            2057..>2901
                     /locus_tag="PKN25_15170"
     CDS             2057..>2901
                     /locus_tag="PKN25_15170"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNV92293.1"
                     /translation="MSAIVDVIAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTG
                     SREAIELRDGDKSRFLGKGVLRAVENVNTEIAEAIIGLDAEEQAFIDRTLIELDGTEN
                     KSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSSPMVMPVPMMNVINGGEHANNSL
                     DIQECMIMPVSMSSFREALRCGAEIFHHLKKIVDAKGYPTTVGDEGGFAPNVSGTEEA
                     LNLIQEAIVAAGYVPGQDVLLALDCAASEFYKDGKYELKGEGLSLTAAGFTDYLETLC
                     DKFPIV"
BASE COUNT          517 a          979 c          944 g          461 t
ORIGIN      
        1 ctgcgcgaga tcggcatcca gcccgacatc ctgctgtgcc gcgccgaccg tccggtgccg
       61 gaagacgagc gccgcaagat cgcgctgttc tgcaacgtga tgcccgaggc ggtcatcgag
      121 tgcctggacg ccgactcgat ctacaagatc cccggtcagc tgcatgacca gatgctcgac
      181 cagatcgtct gccacaagct cgacatcctc gcgcgcgcgg ccgacctctc ggtgtgggac
      241 aggctcatcc acgcgctcga gaacccgaag cagaccgtgg atatcggttt cgtcggcaag
      301 tacgtggacc ttaccgagtc gtacaagtcg ctgatcgagt cgctcaacca cgccggcatg
      361 cataccgagt cgaaggtcag gatccactac atcgactcgg aggatatcga gcgcgacggt
      421 tgcgatgtgc tcgagtcgat ggatgccatc ctggtgccgg gcggcttcgg caagcgcggg
      481 accgagggca agatcgcggc catccgctat gcgcgcgaga acaaggtgcc ctatctgggc
      541 atctgtctcg gcatgcagct cgcggtggtg gagttcgccc gcgacgtcgc gggcatggaa
      601 ggcgcgcact cgaccgagtt cgagcgcgac accaggttcc cggtgatcgg cctcatcacc
      661 gagtggaagg accgcacggg caagatcgag aagcgctccg aggagtccga cctcggcggc
      721 accatgcgcc tcggtggcca gctctgcctg ctcaaggaag gcacgctggc gcgttcgatc
      781 tacggcgccc ccgaggtcat ggagcgtcac cgccaccgct acgaggtgaa caacaccctg
      841 ctcgccaggc tcgaagacaa gggcctggtg gtgtcgggcc gcgcgcccgt caccgacctg
      901 tgcgagatgg tcgagctgcc tgcggacgtg catccgtggt tcgtcggctg tcagttccac
      961 cccgaattca cctccaaccc gcgcaagggt catccgctgt tcaccgctta tgtgaaggct
     1021 gcgatcgcgc acaagcaggc tgcggccccg aggagcgaag catgaatctc tgcggtttcg
     1081 aagtcggcct cgacaagccg gtgttcctga tctccggccc ctgcgtggcc gaatccgagc
     1141 agatgtgcct ggacatcgcc gggcagatga aggagatctg cgccgagctg ggcatccctt
     1201 acatcttcaa ggcttcgtac gacaaggcca accgcagctc gggcaagagc ttccgcggcc
     1261 atggcatgga cgccggcctg aagatgctcg aggcggtcaa gaagcagctc ggcctgccgg
     1321 tgctgaccga cgtgcacacg atcgaggaga tcccgaccgt cgccgcggtg gtcgacgtgc
     1381 tgcagacccc ggccttcctg tgccgccaga ccgatttcat ccacgcggtg gcggccagcg
     1441 gcaagccggt gaacatcaag aagggccagt tcctcgcccc cggcgacatg aagaacgtcg
     1501 ccgacaaggc gcgcgaggcc aatggcggcg ccgacaccat catggtgtgc gagcgcggcg
     1561 cctccttcgg ctacaacaac ctcgtctccg acatgcgtgc gctggcgatc atgcgcgaca
     1621 ccggctgccc ggtggtgttc gacgctaccc actcggtgca gctgccgggc gggcagggaa
     1681 ccgcctcggg tggccagcgc gagttcgtgc cggtgctggc gcgcgccgcg gtcgccgtgg
     1741 gcgtggcggg ccttttcatg gagacccacc cggaccccgc caaggcgctg tccgacggcc
     1801 ccaacgcctg gccgctgccg aagatgaagg cgctgctgac cgtgctcaag gacatcgacg
     1861 cgctggtcaa gcgccagggt ttcctcgaga ccacgctctg agaccccatt tttcgggaat
     1921 gcggctaccg gcggactgat gcgcgtcatc cgtcgccgct gctcacgagg cagtcatccg
     1981 gtgcgagcat gctccccgcc gggtcgcgtt cccgcatgat cgatccgaaa cctccgtaac
     2041 tgctacagga ctagacatga gtgcaatcgt tgatgtgatc gcccgcgaga ttctcgattc
     2101 ccgcggcaac cccacggtcg aagccgacgt gctgctcgaa tcgggcgtga tgggccgcgc
     2161 cgcggtgccc tcgggcgcct ccaccggctc gcgcgaggcg atcgagctgc gtgacggcga
     2221 caagtcgcgc ttcctcggca agggcgtgct gcgcgcggtc gagaacgtga acaccgagat
     2281 cgccgaggcc atcatcgggc tggacgccga ggagcaggcc ttcatcgacc gtaccctgat
     2341 cgagctcgac ggcaccgaga acaagtcgcg cctgggcgcc aacgccatgc tggcggtgtc
     2401 catggcggtg gccaaggccg ccgccgaaga ggccggcctg ccgctgtacc gctacttcgg
     2461 cggctccagc ccgatggtga tgccggtgcc gatgatgaac gtcatcaacg gcggcgagca
     2521 cgccaacaac agcctcgaca tccaggaatg catgatcatg ccggtgagca tgagcagctt
     2581 ccgcgaggcc ctgcgctgcg gcgccgagat cttccaccat ctcaagaaga tcgtcgacgc
     2641 caagggctac cccaccaccg tcggcgacga gggcggcttc gctcccaacg tgtcgggcac
     2701 cgaggaagcg ctcaacctga tccaggaggc catcgtcgcc gccggctacg tgcctggcca
     2761 ggacgtgctg ctggcgctcg actgcgccgc ctccgagttc tacaaggacg gcaagtacga
     2821 gctgaagggc gaaggcctgt cgctgaccgc cgcgggcttc accgactacc tcgagaccct
     2881 gtgcgacaag ttcccgatcg t
//
LOCUS       DAOQBS010000227         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_43726_length_2737_cov_17.545861, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000227 DAOQBS010000000
VERSION     DAOQBS010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43726_length_2737_cov_17.545861"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKN25_15175"
     CDS             complement(<1..395)
                     /locus_tag="PKN25_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNV92294.1"
                     /translation="MAEFLFTSESVSEGHPDKVADQVSDGVLDAVLAEDPKARVACET
                     LVSTGLVVISGEITTTAHPNYREIAQDVIRRIGYDNSDIGFDYKSCAVLAAINRQSPD
                     IAQGVNNANDDPLDQGAGDQGLMFGYATN"
     gene            644..1588
                     /locus_tag="PKN25_15180"
     CDS             644..1588
                     /locus_tag="PKN25_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV92295.1"
                     /translation="MLSQSAFRLLSRLPLSWLHRLGGWAGWLTYKASPSYARRVRENL
                     FNAIGREDETVLRAAIVEAGRQALELPFIWGRPASEVVASAVRTEGWELVEAARAEGA
                     GILFITPHLGCFEITAQCIAARIPITVLYRPPRKEVLQPLMEAGRARGQMRTAPADLS
                     GVRKLVKTLRSHEAVGMLPDQVPGAGEGVWSTFFGKPAWTMTLAARLAAVKGVRVIYT
                     WAERLPRGEGYVFRLQAPAEALTGDIDTDVAIINREVERMILQCPQQYLWGYNRYKGP
                     RREREDAAPQGDAGADAANPSATTSAADTAGTDAERAP"
     gene            1585..2457
                     /locus_tag="PKN25_15185"
     CDS             1585..2457
                     /locus_tag="PKN25_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV92296.1"
                     /translation="MSRLVIGLIWLLHWLPLVLLARVGSGFGRLLYWVVVPRRKVALT
                     NLRLCFPELSEPERRALARRNFALIGRSMLERGLAWWASPERLRATVRIEGLAHLQRL
                     RAEGRPVLLLAPHFLGLDMAGTRLTLEGDFVSVYSPQKDKVFDHWLHHGRSRFNDQLL
                     LSRHDGVRATVKAMKSGRPFYYLPDLDYGRKESIFVPFFGVQAATITGLPRLARLAGA
                     AVVPCITRILPGGQGYVLELGAPWADYPSDDVEADTLRMNAWLEGVVRTMPEQYYWVH
                     RRFKTRPEGEPRIY"
     gene            2475..>2737
                     /locus_tag="PKN25_15190"
     CDS             2475..>2737
                     /locus_tag="PKN25_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNV92297.1"
                     /translation="MKLRFTKMHGLGNDFVVIDATRAPVDLTPARVKAIADRHFGVGC
                     DQLLVVEPAARPDVDFRYRIFNADGGEVEQCGNGARCFVRFVH"
BASE COUNT          382 a          952 c          956 g          447 t
ORIGIN      
        1 tcgttggtgg cgtagccgaa catcaggccc tggtcgccgg cgccctggtc gagcgggtcg
       61 tcgttggcgt tgttcacgcc ctgggcgatg tcgggcgact ggcggttgat cgccgccagc
      121 accgcgcagc tcttgtagtc gaagccgatg tcggagttgt cgtagccgat gcggcggatc
      181 acgtcctgcg cgatctcgcg gtagttgggg tgggcggtgg tggtgatctc gcccgagatc
      241 accaccaggc cggtggacac cagggtctcg caggcgacgc gcgccttggg gtcttcggcc
      301 agcacggcgt cgaggacgcc gtcggagacc tggtcggcga ccttgtcggg atggccttcg
      361 gagaccgatt cggaggtgaa gagaaattca gccattcgtg ctgctccttg gaaaacaaaa
      421 atccccgttg ccatggcctt gcgtctgcgg ccagctccgg ggatttcgtc gagcagccga
      481 cgctttagca gtacttggtt cgggcggcac cgcggccgcc ccaccgccct gcaagttgtc
      541 gagttaactc ggcggtggag cgcggattat agaccgggac gcccggctgt gtaagcattc
      601 gggcctgaag ccggttccgg catccatatc ggatctcaac ctggtgctgt cgcaaagcgc
      661 attccgcctc ctctctcgcc tgcccctgtc ctggctgcac cgcctcggtg gctgggcggg
      721 ctggctgacc tacaaggctt cgccgtccta cgcccgccgc gtgcgcgaga acctcttcaa
      781 cgccatcggt cgcgaggacg agacggtgct gcgtgcggcg atcgtcgagg ccggccgcca
      841 ggcgctggag ctgcccttca tctggggccg gccggcgtcc gaggtggtgg cgagcgcggt
      901 gcgcaccgag ggctgggagc tggtcgaggc cgcgcgcgcc gagggcgccg gcatcctctt
      961 catcaccccg cacctgggct gcttcgagat caccgcccag tgcatcgccg ccaggattcc
     1021 gatcaccgtg ctctatcgcc ctccgcgcaa ggaggtgctg caaccgctga tggaggcggg
     1081 ccgggcgcgc ggacagatgc gtaccgcgcc ggccgatcta tccggcgtgc gcaagctggt
     1141 caagaccctg cgcagccacg aggcggtggg catgctgccc gaccaggtgc cgggcgcggg
     1201 cgagggcgtg tggtcgacct tcttcggcaa gccggcgtgg acgatgacgc tggccgcgcg
     1261 cctggcggcg gtgaagggcg tgcgcgtgat ctacacctgg gccgagcgcc tgccgcgcgg
     1321 cgagggctac gtgttccgcc tgcaggcgcc tgccgaggcg ctcaccggcg acatcgacac
     1381 cgacgtcgcg atcatcaacc gcgaggtcga gcgcatgatc ctgcagtgcc cgcagcagta
     1441 cctgtggggc tacaaccgct acaagggtcc gcggcgcgag cgcgaggacg ccgccccgca
     1501 gggggatgcg ggcgccgatg ccgccaaccc ctcggcgacc acttccgccg ccgacaccgc
     1561 cggcacggac gcggagcgcg cgccatgagc cgcctggtca tcggcctgat ctggctgctg
     1621 cactggctgc cgctggtgct gctcgcacgc gtgggaagcg gcttcggccg gctgctctac
     1681 tgggtggtgg tgccgcgccg caaggtcgcg ctgaccaacc tgcggctgtg ctttcccgag
     1741 ctctccgagc ccgaacggcg cgcgctggcg cggcgcaact tcgccctcat cggacgcagc
     1801 atgctcgagc gcggcctggc ctggtgggcg agccccgagc gcctgcgcgc caccgtccgc
     1861 atcgagggcc tggcgcacct gcagcgcctg cgcgccgagg gccgcccggt gttgctgctg
     1921 gcgccgcact tcctcggcct cgacatggcc ggcacccggc tcacgctcga gggcgacttc
     1981 gtcagcgtgt attcgccgca gaaggacaag gtcttcgacc actggctgca ccatggccgc
     2041 agccgcttca acgaccagct gctgctgtcg cgccacgatg gcgtgcgcgc cacggtcaag
     2101 gcgatgaagt ccggccgccc tttctattac ctgcccgacc tcgactacgg ccgcaaggag
     2161 tccatcttcg tgcccttctt cggcgtgcag gcggcgacca tcaccggcct gccgcgcctg
     2221 gcgcgcctgg cgggtgcggc ggtggtgccc tgcatcaccc gcatcctgcc cggcggacag
     2281 ggctacgtgc tggagctcgg cgcgccctgg gccgactacc cttcggacga cgtcgaggcc
     2341 gacacgctgc gcatgaacgc ctggctcgaa ggcgtggtgc gcaccatgcc cgagcagtac
     2401 tactgggtgc accgccgctt caagacccgg cccgagggcg agccgcggat ctactgaaac
     2461 actggaacag caagatgaaa ctgcgcttca ccaagatgca cggcctgggc aacgacttcg
     2521 tcgtcatcga cgccacgcgt gcgccggtcg acctcacccc ggcgcgggtc aaggcgatcg
     2581 ccgaccgcca tttcggcgtc ggctgcgatc agctgctggt ggtcgagccc gccgcgcggc
     2641 cggatgtcga tttccgctac cgcatcttca acgccgatgg cggcgaggtc gagcagtgcg
     2701 gcaacggcgc gcgctgcttc gtgcgcttcg tgcatga
//
LOCUS       DAOQBS010000228         2727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_43984_length_2727_cov_16.626123, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000228 DAOQBS010000000
VERSION     DAOQBS010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43984_length_2727_cov_16.626123"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /gene="trpB"
                     /locus_tag="PKN25_15195"
     CDS             <1..1079
                     /gene="trpB"
                     /locus_tag="PKN25_15195"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNV92298.1"
                     /translation="DPAFVAEFEYELKHYVGRPSPIYHAKRWSELLGGAQIYLKREDL
                     NHTGAHKVNNCIGQAMVARRMGKPRVIAETGAGQHGVATATVAARYGMECVVYMGSED
                     VKRQAANVYRMKLLGATVVPVESGSKTLKDALNEAMRDWVTNVADTFYIIGTVAGPHP
                     YPMMVRDFQSVIGKECLVQMPDMVGRQPDYVIACVGGGSNAMGIFHPYIGVPGVRLVG
                     VEAAGDGLDTGRHSASLTAGRPGVLHGNRTYLLQDEDGQIIETHSVSAGLDYPGVGPE
                     HAWLKDTARAEYVGITDAEALEAFHKLCRLEGIIPALESSHALAYAAKLAPSLPKDKI
                     LLVNLSGRGDKDMHTVAERSGIQF"
     gene            1135..1956
                     /gene="trpA"
                     /locus_tag="PKN25_15200"
     CDS             1135..1956
                     /gene="trpA"
                     /locus_tag="PKN25_15200"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNV92299.1"
                     /translation="MSRIQTVFQRLQADGRKALIPFVTAGFPAPALTLPLMNALVEGG
                     ADILELGVPFSDPMADGPTIQRASERALAQGMSLRKVLETVRAFRAANADTPVVLMGY
                     ANPIEAMGQERFVAAAHEAGVDGVLVVDYPPEECVSFAATAKAGGLDPIFLLAPTSGE
                     QRFADVARAGSGYIYYVSLKGVTGSAKLDVDEVARRIPEIRAAVGMPVGVGFGIRDAE
                     SAQRIGAVADAVVIGSRIIEEIENNPGDEVARVRDFMRPIRAALDALATGRGVQA"
     gene            1953..>2727
                     /gene="accD"
                     /locus_tag="PKN25_15205"
     CDS             1953..>2727
                     /gene="accD"
                     /locus_tag="PKN25_15205"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNV92300.1"
                     /translation="MSWFEKLIPPRIKRGDSSRSKSIPEGLWSKCEGCESVLYRSDLD
                     SNLGVCPKCGHHHRLRARRRLDQLLDPEGRVEIGAEVTPVDALKFKDSRKYPDRLSAA
                     AADTGEADAMVVMRGAIKTVPVVVACFEFEFLGGSMGSVVGERFVRGARAAFEERLPF
                     ICITASGGARMQEGLLSLMQMAKTTASITRLAERRLPFITLLTDPTMGGVSASFAFMG
                     DVVVAEPNALIGFAGPRVIEQTVREKLPEGFQRAEFLLEK"
BASE COUNT          424 a          976 c          910 g          417 t
ORIGIN      
        1 acgatccggc gttcgtcgcc gagttcgagt acgagctcaa gcactacgtc ggccgcccga
       61 gcccgatcta ccatgccaag cgctggtcgg agctccttgg cggcgcgcag atctacctca
      121 agcgcgagga tctcaaccac accggcgcgc acaaggtgaa caactgcatc ggccaggcca
      181 tggtcgcccg ccgcatgggc aagccgcgcg tgatcgccga gaccggtgcc ggccagcacg
      241 gtgtggccac cgccaccgtg gccgcacgct acggcatgga gtgcgtggtc tacatgggtt
      301 cggaggacgt caagcgccag gccgccaacg tctatcgcat gaagctgctc ggcgccaccg
      361 tggtgccggt cgagtccggc tcgaagacgc tcaaggatgc gctcaacgaa gccatgcgcg
      421 actgggtgac caacgtcgcc gataccttct acatcatcgg caccgtcgcc ggcccgcacc
      481 cgtatccgat gatggtgcgc gacttccagt cggtgatcgg caaggaatgc ctggtgcaga
      541 tgcccgacat ggtgggccgc cagcccgact acgtgatcgc ctgcgtgggc ggcggctcca
      601 acgcgatggg catcttccat ccctacatcg gcgtgccggg cgtcaggctt gtcggcgtcg
      661 aagccgccgg cgacggcctg gacacggggc gccactcggc ctcgctgacc gccggccgcc
      721 ccggtgtgct gcacggcaac cgcacctacc tgctgcagga cgaggacggg cagatcatcg
      781 agacgcactc ggtctccgcc ggactcgact acccgggcgt cggtcccgag cacgcctggc
      841 tgaaggacac cgcacgcgcc gagtatgtcg gcatcaccga cgccgaggcg ctcgaggcct
      901 tccacaagct gtgccggctg gagggcatca ttccggcgct cgagtcctcg cacgcgctcg
      961 cctacgccgc caagctggcg ccctccctgc ccaaggacaa gatcctgctg gtcaacctct
     1021 ccgggcgcgg cgacaaggac atgcacaccg tcgccgagcg ctccggcatc cagttctgaa
     1081 cctgaatccg aacctgaagc gggccgctga tgcggcccgc agtgtgatcg agacatgtcg
     1141 agaatccaga ccgttttcca gcgcctccag gccgatggcc gcaaggcgct gattcccttc
     1201 gtcaccgccg gcttcccggc gcccgcgctc accctgccgc tgatgaacgc gctggtcgag
     1261 ggcggcgccg acatcctcga gctcggcgtg cccttctccg acccgatggc cgacggcccc
     1321 accatccagc gcgcctccga gcgcgcgctc gcgcagggca tgagcctgcg caaggtgctc
     1381 gagaccgtgc gcgccttccg cgccgccaat gccgataccc cggtggtgct gatgggctac
     1441 gccaacccga tcgaggccat gggccaggag cgcttcgtcg ctgccgcgca tgaagccgga
     1501 gtcgatggcg tgctggtggt cgattacccg ccagaggagt gcgtgagctt cgccgccacc
     1561 gccaaggccg gcggcctcga tcccatcttc ctgctcgcgc ccacctcggg cgagcagcgc
     1621 ttcgccgatg tcgcgcgcgc cggcagcggc tatatctact acgtctcgct caagggcgtg
     1681 accggcagcg ccaagctcga cgtcgacgag gtcgcccgcc gcattcccga gatccgcgcc
     1741 gcggtcggca tgccggtggg cgtgggcttc ggcatccgcg acgccgagag cgcgcagcgc
     1801 atcggcgcgg tggccgacgc ggtcgtgatc ggcagccgga tcatcgagga gatcgagaac
     1861 aacccgggtg acgaggtggc gcgtgtgcgc gacttcatgc gcccgatccg cgccgcgctg
     1921 gacgcgctgg cgacgggcag gggggtacag gcatgagctg gttcgagaaa ctgatcccgc
     1981 cgcgcatcaa gcgcggcgac tcgtcgcgca gcaagtcgat cccggaaggg ctgtggagca
     2041 agtgcgaggg ttgcgagtcg gtgctctacc gctccgacct cgacagcaac ctcggcgtgt
     2101 gcccgaagtg cggccatcac catcgcctgc gcgctcgccg tcgcctcgac cagctgctcg
     2161 accccgaggg ccgcgtcgag atcggcgccg aggtcacgcc ggtcgatgcg ctcaagttca
     2221 aggactcgcg caagtatccc gatcgcctgt ccgcggccgc tgccgacacc ggcgaggccg
     2281 acgcgatggt ggtgatgcgc ggcgcgatca agaccgtccc ggtggtggtc gcctgcttcg
     2341 agttcgaatt cctcggcggc tcgatgggtt cggtggtcgg cgagcgcttc gtgcgcggtg
     2401 cgcgtgcggc cttcgaggag cgcctgccct tcatctgcat caccgcctcg ggcggcgcgc
     2461 gcatgcagga aggcctgctc tcgctgatgc agatggccaa gaccaccgcc tcgatcaccc
     2521 gcctggccga gcgccgcctg cccttcatca ccttgctgac cgaccccacc atgggcggcg
     2581 tgtcggcgag ctttgcattc atgggcgacg tggtcgtcgc cgagcccaac gcgctgatcg
     2641 gcttcgccgg cccgcgcgtg atcgagcaga ccgtgcgcga gaagctgccc gaaggcttcc
     2701 agcgcgccga gttcctgctc gagaagg
//
LOCUS       DAOQBS010000229         2633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_46405_length_2633_cov_13.138479, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000229 DAOQBS010000000
VERSION     DAOQBS010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46405_length_2633_cov_13.138479"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..130
                     /gene="rpsU"
                     /locus_tag="PKN25_15210"
     CDS             <1..130
                     /gene="rpsU"
                     /locus_tag="PKN25_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNV92301.1"
                     /translation="LTELRSREFYEKPTAERKRKLAAAVKRNHKRLRSQTLPPKLY"
     gene            291..2186
                     /gene="dnaG"
                     /locus_tag="PKN25_15215"
     CDS             291..2186
                     /gene="dnaG"
                     /locus_tag="PKN25_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNV92302.1"
                     /translation="MIPQSFVQELLSRVDIVDVIERYLPLKKSGANYFACCPFHGEKS
                     ASFSVSPSKQFYHCFGCGVHGSAVGFLMEYSGLGFVDAIKELAAQVGLQVPDDGRSAA
                     PVHDDASERLYEAMAVAARFYRDQLKPSRQAIDYLKRRGLSGEVAARFGLGYAPDEWQ
                     ALEKAFPDYQAKTLADAGLVIDNEQGRRYDRFRNRIIFPIHDRRGRIIAFGGRVLDKG
                     EPKYLNSPETPLFEKGRELYGLFLAQKAIRDAGFAVVVEGYMDVVALAQFAIENAVAT
                     LGTATTPQHIHTLLRQTDRIVFCFDGDAAGRRAAWRALENALESLRDDATLAFLFLPA
                     EHDPDSFVRAEGTEAFRKAALAATPLGSFLIQELSGRHPLDSAEGRAAFVHEAAPLVT
                     RIAAPLLKLQVVKAVAESAGMTQGEVEYAFEQLAASAPRRRGASAPTPPRAPAASIAS
                     TEADAPFFDDAPPPARSAPPRGQAPRPRAGGRRKPPSTAGTLLRLVMQHPTWAARMPV
                     GLVPDDSPEGLALIAIIDLCSLGEPIPSGGLGALIERFRETPHAETLSRVAAELVEAE
                     FDEAVVETLFEDALRKLQIDGVGKEITALLQRDREQGLDAAGRHRLGELLLEKRNLAS
                     SGKVSDL"
     gene            2236..>2633
                     /locus_tag="PKN25_15220"
     CDS             2236..>2633
                     /locus_tag="PKN25_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HNV92303.1"
                     /translation="MAREKAKDPRKDAPAKGRPAKAKDKAAPVVESPAATPLDAEVRR
                     TRLKTLITLGKERGYLTYAEISDHLPDDVADAEQIEGIIATFNNMGIQVYDEAPAAED
                     LLMSDSVATNVDEDVAEEEAEQALSSVDSE"
BASE COUNT          437 a          999 c          832 g          365 t
ORIGIN      
        1 gctgaccgaa ctgcgctcgc gcgagttcta cgaaaagccc accgccgagc gcaagcgcaa
       61 gctggccgcc gccgtcaagc gcaaccacaa gcgcctgcgc agccagacgc tgccgccgaa
      121 gctctactga tccggtacgc gacccgccct cgcgggacgc ccatgcccgc cctggcgggc
      181 gatcacacgc cggcccgggg tcgctcgatc cccgcccggc gtgatgcttt ttcacgaaca
      241 cccgtcgtcc gcgcatgcgg aagcgacggg cgtttgtctt tgcgcgcgcg atgattcccc
      301 agtccttcgt ccaggaactg ctttcccgcg tcgacatcgt cgacgtcatc gaacgctacc
      361 tcccgctcaa gaagagcggc gccaactact tcgcctgctg ccccttccac ggcgagaaat
      421 ccgcctcgtt ctcggtcagc ccgtccaagc agttctacca ctgcttcggt tgcggcgtgc
      481 acggtagcgc ggtcggcttc ctgatggagt acagcgggct gggcttcgtc gacgcgatca
      541 aggagctcgc tgcccaggtc ggcctgcagg tgcccgacga tggccgcagc gcggcacccg
      601 tgcatgacga cgcgagcgaa cgcctttatg aggcgatggc ggtggcggcg cgcttctacc
      661 gcgaccagct caagccttcc cgccaggcca tcgactacct caagcgccgc gggctgtcgg
      721 gcgaggtcgc cgcgcgcttc gggctcggct acgcgcccga cgagtggcag gcgctggaga
      781 aggccttccc cgactaccag gcgaagacgc tcgccgacgc cggcctggtc atcgacaacg
      841 agcaggggcg gcgctacgac cgcttccgca accgcatcat cttcccgatc cacgaccgcc
      901 gcggacgcat catcgccttc ggcgggcgcg tgctcgacaa gggcgaaccc aagtacctga
      961 actcgcccga gaccccgctg ttcgagaagg ggcgcgagct ctacggcctc ttcctggcgc
     1021 agaaggcgat ccgcgacgcc ggcttcgcgg tcgtggtcga gggctacatg gacgtggtcg
     1081 cgctcgccca gttcgccatc gagaatgcgg tcgccacgct cggcaccgcg accacgccgc
     1141 agcatatcca caccctgctg cgccagaccg accgcatcgt gttctgcttc gacggcgacg
     1201 ccgcaggccg ccgcgcggca tggcgtgcgc tcgagaacgc actcgaatcc ctgcgcgacg
     1261 acgccaccct ggccttcctg ttcctgcccg ccgagcacga cccggactcc ttcgtgcgcg
     1321 ccgagggcac cgaagccttc cgcaaggccg cgctcgccgc caccccgctg gggagcttcc
     1381 tgatccagga gctgtccggc cgccatccgc tcgacagcgc cgagggccgc gcggccttcg
     1441 tgcatgaggc ggcgccgctg gtcactcgca tcgccgcgcc gctgctgaag ctgcaggtgg
     1501 tgaaggcggt ggccgagagc gcgggcatga cccagggcga ggtcgaatac gccttcgagc
     1561 agctcgccgc cagcgccccg cgccggcgtg gcgcctcggc gcccaccccg ccccgcgccc
     1621 ccgccgcgag catcgcaagc accgaggcgg acgccccctt cttcgacgat gcccccccgc
     1681 ccgcccgcag cgcgccgccg cgcggccagg cgccgcgccc gcgcgcgggc ggccggcgca
     1741 agcccccctc caccgccggc accctgctgc gcctggtgat gcagcacccg acctgggccg
     1801 cgcgcatgcc ggtcggcctg gtgcccgacg acagcccgga aggcctcgcg ctgatcgcga
     1861 tcatcgacct ctgcagcctc ggcgagccga tcccctcggg cggactgggc gcgctgatcg
     1921 agcgcttccg cgagacaccc cacgccgaaa ccctctcgcg tgtcgccgcg gaactcgtgg
     1981 aggcggaatt cgacgaagcg gtggtcgaga cgctgttcga ggacgccctg cgcaaactgc
     2041 agatcgacgg cgtcggcaag gagatcaccg ccctgctaca acgggatcgt gagcaaggcc
     2101 tggacgccgc cggccgccat cgcctcggag agctgctgct cgagaagcgg aacctcgcga
     2161 gcagcggcaa agtctcagat ttatagtaca atttcgggtt tccccatttt cttacaccct
     2221 cacttgagga gtgctatggc acgcgaaaaa gccaaggacc cacgcaagga cgcccccgcg
     2281 aagggccgcc ctgccaaggc caaggacaag gcagccccgg tggtcgaaag ccccgccgcc
     2341 accccgctcg acgcggaggt gcgccgcacc cggctgaaga cgctgatcac gctcggcaag
     2401 gaacgcggct atctcaccta cgccgagatc agcgaccacc tccccgacga tgtggccgat
     2461 gccgagcaga tcgaaggcat catcgccacc ttcaacaaca tgggcatcca ggtctatgac
     2521 gaggcgccgg ccgccgaaga cctgctcatg tcggacagcg tcgccaccaa cgtggacgag
     2581 gacgtcgccg aagaggaagc cgaacaggcg ctgtcctcgg tcgactccga att
//
LOCUS       DAOQBS010000230         2414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_53289_length_2414_cov_12.768122, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000230 DAOQBS010000000
VERSION     DAOQBS010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53289_length_2414_cov_12.768122"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..957
                     /gene="xth"
                     /locus_tag="PKN25_15225"
     CDS             193..957
                     /gene="xth"
                     /locus_tag="PKN25_15225"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNV92304.1"
                     /translation="MKIATWNVNSLKVRLPQVLDWLAANQPDALCLQELKLEDKAFPL
                     AELEAAGYHAVFIGQKTYNGVAILSPQPIAEASVVRNIPGFEDEQKRIIAATIAGVRV
                     VCGYFPNGQAVGSDKFEYKLRWLAALTDWLREELKTHERLVLAGDFNIAPEDRDAHPD
                     WKDEIHVSAPERAAFRALQDLGLIDAFRLFEQPERAFSWWDYRMMAFRRNFGLRIDHL
                     LVSPALRTACRSCVVDKEPRRLERPSDHAPVVLELA"
     gene            962..1648
                     /locus_tag="PKN25_15230"
     CDS             962..1648
                     /locus_tag="PKN25_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNV92305.1"
                     /translation="MSASTSPTDAGRLEARYPVLAELSAPARTRLLQSAHWMHVPAGA
                     MLFDDHQACEGFPFVVEGTVRVSKCAPSGRELPLYRVGPGETCIISSSCLLGHEDYNA
                     RGVTESDTLLMLLPKAVFDELMGERPFRDFVFHLFAERIADLMQLIEEVAFRKLDQRL
                     AALLLGKGRVLHVTHQQLADELGSVREIVSRLLKSFAEQGLVKLAREQVEIIDPAGLR
                     RMAAEGKGAA"
     gene            1796..1987
                     /locus_tag="PKN25_15235"
     CDS             1796..1987
                     /locus_tag="PKN25_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004354734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNV92306.1"
                     /translation="MKTNVGGADKILRIVAGIALLAWAVFGGPVWAWIGIVPLATGLL
                     GWCPAYTMLGMNTCPLGKK"
     gene            complement(2055..>2414)
                     /locus_tag="PKN25_15240"
     CDS             complement(2055..>2414)
                     /locus_tag="PKN25_15240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HNV92307.1"
                     /translation="EPGTEEYGRLSVMLQYRFRMGRLFDVPPGAFRPAPKVMSSIVRM
                     APLPAEQLGARDEALLGRIVAAAFGQRRKTLRNTLREFIDDAGLQALGIDPGLRGEKL
                     SVEQYVAIANACAGRQG"
BASE COUNT          372 a          872 c          793 g          377 t
ORIGIN      
        1 taccagatca tcgtgctgcg cttcgtgtat ccgggcgaag tcatcctcgt cgccttcacg
       61 ctggcgatcc tgccctacct gcttctgcgc gggctggtca cccgcattgc caggaggcgc
      121 tgatcgcgag ccggtgactt gtgacttggc ggcttacaat ggcgggcctt gcttcaatcg
      181 ccggttcgac agatgaagat cgccacctgg aacgtcaact cgctcaaggt ccgcctgccc
      241 caggtcctcg actggctcgc cgccaaccag cccgacgcgc tgtgcctgca ggagctcaag
      301 ctcgaggaca aggccttccc gctcgccgag ctcgaagccg ccggctacca cgccgtcttc
      361 atcggccaga agacctacaa cggcgtcgcc atcctgagcc cgcagccgat cgccgaggcc
      421 agcgtggtgc gcaacatccc cggcttcgag gacgagcaga agcgcatcat cgccgccacc
      481 atcgcgggcg tgcgtgtggt gtgcggctac ttccccaacg gccaggcggt gggttcggac
      541 aagttcgagt acaagctgcg ctggctcgcg gcgctgaccg actggctgcg cgaggagctg
      601 aagacgcacg aacgccttgt gctggccggc gacttcaaca tcgcaccgga agaccgcgac
      661 gcccaccccg actggaagga cgagatccac gtgtcggcgc ccgagcgcgc cgccttccgc
      721 gcgctgcagg acctcggcct gatcgacgcc ttccgcctct tcgagcagcc cgagcgcgcg
      781 ttctcgtggt gggactaccg gatgatggcc ttccggcgca acttcggcct gcgcatcgac
      841 cacctgctgg tgtcgccggc gttgcgcacg gcatgccgca gctgcgtggt cgacaaggaa
      901 ccgcgccggc tggagcggcc gtcggaccac gcgccggtgg tgctggaact ggcctgaagc
      961 gatgagcgcc agcacctccc cgaccgacgc cgggcgcctc gaagcccgct acccggtgct
     1021 ggccgagctg tcggcaccgg cgcgcacccg cctgctgcaa agcgcgcact ggatgcacgt
     1081 gcccgcgggc gcgatgctgt tcgacgacca tcaggcctgc gagggctttc ccttcgtggt
     1141 cgagggcacg gtgcgggtga gcaagtgcgc gccgagcggg cgcgagctgc cgctctaccg
     1201 cgtcggcccg ggcgagacct gcatcatctc ctcgtcctgc ctgctcggcc acgaggacta
     1261 caacgcgcgc ggcgtcaccg agtccgacac cctgctgatg ctgctgccga aggcggtgtt
     1321 cgacgagctg atgggcgaac gccccttccg cgacttcgtc ttccacctgt ttgccgagcg
     1381 catcgccgac ctgatgcagt tgattgagga ggtcgccttc cgcaagctcg accagcgcct
     1441 ggcggcgctg ctgctcggca agggccgggt gctgcacgtc acccaccagc agctcgccga
     1501 cgagctcggc agcgtgcgcg agatcgtcag ccggctgctc aagagcttcg ccgagcaggg
     1561 cctggtgaag ctcgcgcgcg agcaggtcga gatcatcgac ccggcggggc tgcgccgcat
     1621 ggccgccgag ggcaagggcg cggcctgagc cacctacctc cgggatcccg ctcccgggcg
     1681 gaccggcgta gcatcccgga tcgggtacgg gctgtgtaac ctcggttaca gaccgcagcc
     1741 ggccgggcgt ggtccaatcc ggccactcac ctcatccgat ccaaggagaa cacacatgaa
     1801 aacgaacgtt ggtggcgccg acaagatcct tcgcatcgtg gccggtatcg cgctgctcgc
     1861 ctgggcggtg ttcggcggcc cggtgtgggc ctggatcggc atcgtcccgc tcgccaccgg
     1921 cctgctcggc tggtgccccg cctatacgat gctgggcatg aacacctgcc cgctcggcaa
     1981 gaagtaagca cgccccaccc cacgaaaaag ccggcgcacc gccggctttt ttacgcgcct
     2041 ctcgacttgg cgcctcaccc ctgtcgcccc gcgcacgcat tcgcgatcgc gacgtactgc
     2101 tccaccgaca gcttctcgcc acgcagcccg gggtcgatcc cgagcgcctg cagcccggcg
     2161 tcgtcgatga actcgcgcaa ggtgttgcgc agggtcttgc ggcgctggcc gaaggcggcg
     2221 gcgacgatgc gcccgagcaa ggcctcgtcg cgcgcgccga gctgttccgc cggcagaggc
     2281 gccatgcgca cgatgctgga catcaccttg ggcgccggac ggaaggcgcc cggcggcacg
     2341 tcgaagaggc gccccatgcg gaagcggtac tgcagcatca ccgacagccg tccgtactcc
     2401 tcggtgccgg gctc
//
LOCUS       DAOQBS010000231         2333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_56220_length_2333_cov_20.242318, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000231 DAOQBS010000000
VERSION     DAOQBS010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56220_length_2333_cov_20.242318"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="PKN25_15245"
     CDS             <1..1290
                     /locus_tag="PKN25_15245"
                     /inference="COORDINATES: protein motif:HMM:NF006631.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtzE family amidohydrolase"
                     /protein_id="HNV92308.1"
                     /translation="PKVNAFTAVLAERALKRAGQIDKHTRRAELSLAGVPFAVKNLFD
                     IAGLPTLAGSKIERDAAPASRDALLVRRMEAAGAILVGALNMDEYAYGFTTENTHYGP
                     TRNPHDPARISGGSSGGSAAAVAAGQVPLTLGSDTNGSIRVPSSLCGVFGLKPTYGRL
                     PRLGSYPFVASLDHLGPFARSVADLAFAYDAMQGPAPDDPACAQRPPEPTRDLLERRA
                     DGLRIAVLGGWFREMAMPEALAAVDAVAQALGTTRLVELHDVDRARAAAFLITNAESS
                     TLHLADLKQRAQDFEPLSRDRFLAGALLPAAWVVQAQRVRHAFARQLARAFETVDVLL
                     APATPCVAPVIGTEWLEINGQRLPSRPSMGLLTQPISCIGLPVVTVPLWGCNPAAPHL
                     PIGVQVIAAPWREDLALRVAAELEAQGLATAPVAALN"
     gene            1298..1699
                     /gene="hpxZ"
                     /locus_tag="PKN25_15250"
     CDS             1298..1699
                     /gene="hpxZ"
                     /locus_tag="PKN25_15250"
                     /inference="COORDINATES: protein motif:HMM:NF022968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalurate catabolism protein HpxZ"
                     /protein_id="HNV92309.1"
                     /translation="MDINLPDVHAEVSAIFALYEQALTSNDVTLLDQLFWDSPHTVRY
                     GVGENLIGIEAIRAFRNARSPVGLARELRHTVITTFGRDFATAMTEFARPAARGRQSQ
                     TWVRTAAPGHGGWRVVAAHVSLLPQPAPIQP"
     gene            1714..>2333
                     /locus_tag="PKN25_15255"
     CDS             1714..>2333
                     /locus_tag="PKN25_15255"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV92310.1"
                     /translation="MSSHPTHDALRRRLLAGTALGAGSLALPGALREALAQTPLVVGV
                     IYVGPRGDFGYNQAQAQAAAAIKKLPGVKVVEEENVPETAAVQKTMEAMINQDKASLI
                     FPTSFGYFDPHMLKMAEKYPKVRFAHCGGMWTEGKHPKNTGSYFGYIDECQYLAGIVA
                     GYTSKTKKLGFIAAKPIPQVLRNINAFTMGARSVDPSITTRVIFTG"
BASE COUNT          338 a          913 c          764 g          318 t
ORIGIN      
        1 ccgaaggtca acgccttcac cgccgtgctc gccgagcgtg cgctcaagcg cgccgggcag
       61 atcgacaagc acacgcgccg cgccgagctg tcgctggctg gcgtgccctt cgcggtgaag
      121 aacctgttcg acatcgccgg cctgcccacg ctcgccggct cgaagatcga acgcgacgcc
      181 gcgcccgcct cgcgcgacgc gctgctcgtg cgccgcatgg aggcggccgg cgccatcctc
      241 gtcggcgcgc tcaacatgga cgagtacgcc tacggcttca ccaccgagaa cacgcactac
      301 ggcccgacgc gcaacccgca cgacccggcg cgcatctccg gcggctcgtc cggcggctcg
      361 gccgccgcgg tggccgcggg gcaggtgccg ctcacgctgg gatccgacac caacggctcg
      421 atccgcgtgc cctcgtcgct gtgcggcgtg ttcggcctca agcccaccta cggccgcctg
      481 ccgcggttgg gcagctaccc cttcgtggcc agcctcgacc acctcggccc cttcgcgcgc
      541 agcgtggccg acctcgcctt cgcctacgac gccatgcagg gccccgcgcc ggacgatccg
      601 gcctgcgcgc agcgcccgcc cgagccgacg cgcgatttgc tcgagcgccg cgccgacggg
      661 ttgcgcatcg ccgtgcttgg cggctggttt cgcgagatgg cgatgcccga ggcgctggcc
      721 gcggtcgacg ccgtcgcgca ggcgctgggc accacgcggc tggtcgaact gcacgatgtc
      781 gaccgtgcgc gcgccgccgc cttcctgatc accaatgccg agagctcgac gctgcacctg
      841 gccgacctga agcagcgcgc gcaggatttc gagccgctgt cgcgcgaccg cttcctcgcc
      901 ggcgctctgc tgcccgccgc ctgggtcgtg caggcgcagc gggtgcgcca cgccttcgcg
      961 cggcagctgg cgcgcgcctt cgagacggtc gacgtgctgc tcgcgccggc cacgccctgc
     1021 gtcgcgccgg tcatcggcac cgagtggctc gagatcaacg gccagcgcct gccgtcccgc
     1081 ccgagcatgg gtctgctgac gcagccgatc tcctgcatcg gcctgccggt ggtcaccgtg
     1141 ccgctgtggg gctgcaaccc cgcggcaccg cacctgccca tcggcgtgca ggtgatcgcc
     1201 gcgccctggc gcgaagacct cgcgctgcgc gtggccgccg agctcgaggc acagggcctc
     1261 gccaccgccc ccgtggccgc cttgaactga gccgcccatg gacatcaacc tgcctgacgt
     1321 gcacgccgaa gtcagcgcga tcttcgcgct gtacgaacag gcgctgacaa gcaacgacgt
     1381 gacgctgctc gatcagcttt tctgggattc gccgcacacc gtgcgctacg gcgtcggcga
     1441 gaacctgatc ggcatcgagg cgatccgcgc cttccgcaac gcgcgttcgc cggtcggcct
     1501 ggcgcgcgag ctgcgccaca ccgtcatcac caccttcggc cgcgacttcg ccaccgcgat
     1561 gaccgagttc gcccgccccg ccgcgcgcgg ccgccagagc cagacctggg tgcgcaccgc
     1621 cgcgcccggc catggcggct ggcgcgtcgt ggccgcgcac gtgagcctgt tgccccagcc
     1681 cgcccccatc caaccctgaa cgaggagaac cccatgagca gccaccccac ccacgatgcc
     1741 ctgcgccgcc gcctgctcgc cggcaccgcc ctcggcgccg gctcgctggc gctgcccggc
     1801 gcgctgcgcg aggcgctggc gcagacgccg ctcgtcgtcg gcgtgatcta cgtcggcccg
     1861 cgcggcgact tcggctacaa ccaggcgcag gcgcaggccg ccgcggcgat caagaagctg
     1921 cccggcgtga aggtggtcga ggaagagaac gtgcccgaga ccgccgccgt gcagaagacc
     1981 atggaggcga tgatcaacca ggacaaggcc tcgctgatct tcccgacctc gttcggctac
     2041 ttcgacccgc acatgctgaa gatggcggag aagtacccca aggtgcgctt cgcgcattgc
     2101 ggcggcatgt ggaccgaagg caagcacccg aagaacaccg gcagctactt cggctacatc
     2161 gacgagtgcc agtacctggc cggcatcgtg gcgggctaca ccagcaagac caagaagctc
     2221 ggcttcatcg ccgccaagcc gatcccgcag gtgctgcgca acatcaacgc cttcacgatg
     2281 ggcgcgcgct cggtcgaccc gagcatcacc acgcgggtga tcttcaccgg cga
//
LOCUS       DAOQBS010000232         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_57269_length_2306_cov_15.997335, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000232 DAOQBS010000000
VERSION     DAOQBS010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57269_length_2306_cov_15.997335"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="PKN25_15260"
     CDS             <1..1635
                     /locus_tag="PKN25_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV92311.1"
                     /translation="VMPMLLRSRRLDLARERDLTGLLLGIRRGAIVVLLLLGYLYFRL
                     AGEAYALVSIGLISFAAVAQFAPVVIGGMYWRGGTREGALAGLLAGFAVWVYTLLLPS
                     FAKSGWLDPGFLEHGVLGLEWLKPEQLFGLTGLDNISHALFWSLLANIGCYVAVSMLR
                     VPTGPEATQGALFVDVFRSGAAAPASFWRTGAEAGELVPLVARFLGQRRTEEAFGAYA
                     RSHGLQQVSELKADPELVHFAETLLAGAIGSASARVMVSTVVQAEPLGLGEVMDILDE
                     ASQVRAYSHKLEEKSRALEAATAELRAANERLKELDRLKDDFMSSVTHELRTPLTSIR
                     ALSEMMLDDPDIDVEDRQRFLGIIVSEAVRLSRLVNQVLDMAKIESGHAEWHNTDIDL
                     CELVRHSGDATLQLFRDRGAELHLHLPESVPTLRADHDRLLQVMLNLLSNAAKFVPTG
                     SGRVDVTLSCDGERLRVAVKDNGPGIEPAQQPVVFEKFRQGGDERSRPQGTGLGLPIS
                     RQIVEHFGGRLWLESVPGEGATFSFELPLKSAAGEG"
     gene            1666..2034
                     /locus_tag="PKN25_15265"
     CDS             1666..2034
                     /locus_tag="PKN25_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV92312.1"
                     /translation="MDKKVLIADDEQNIVISLEFLMKREGYQVEVANDGEEAVRRIRA
                     SRPDLVLLDVMMPKKSGFEVCQEIRSDPEMAGIRILMLTAKGRDTEVAKGLALGADAY
                     MTKPFSTRELVDKVRSLLEH"
     gene            2038..>2306
                     /locus_tag="PKN25_15270"
     CDS             2038..>2306
                     /locus_tag="PKN25_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HNV92313.1"
                     /translation="MQARARFMLAVVVLFLLMTGPFLLTALIVWVETEGEARDLLLKV
                     LSPHLTLGTLLTAMGFAAGVGVVRVLFRQYVQGLLRMSENLRLMLG"
BASE COUNT          330 a          782 c          816 g          378 t
ORIGIN      
        1 gtgatgccga tgctgctgcg cagccggcgg ctcgacctcg cgcgcgagcg cgacctcacc
       61 ggcctgctgc tgggcatccg ccgcggtgcg atcgtggtgc tgctgctgct cggctacctc
      121 tacttccgcc tcgccggcga ggcctacgcg ctggtcagca tcggcctcat cagcttcgcc
      181 gcggtggcgc agttcgcccc ggtggtgatc ggcggcatgt actggcgcgg cggcacccgc
      241 gagggcgcgc tcgccggtct gctcgccggc ttcgcggtgt gggtctatac gctgctgctg
      301 ccctcgttcg ccaagtcggg ctggctcgat ccgggcttcc tcgagcacgg cgtgctcggc
      361 ctggaatggc tcaagcccga gcagctcttc ggcctcaccg gcctggacaa catcagccac
      421 gcgctgttct ggagcctgct cgccaacatc ggctgctacg tcgcggtatc gatgctgcgc
      481 gtgccgaccg ggccggaggc gacccagggc gcgctctttg tggacgtgtt ccggagcggc
      541 gcggcggcgc cggcgagctt ctggcgcacc ggcgccgagg ccggcgagct ggtgccgctg
      601 gtggcgcgct tcctcgggca gcgccgcacc gaggaggcct tcggcgccta cgcgcgctcg
      661 cacggcctgc agcaggtctc cgagctcaag gccgaccccg agctggtgca cttcgccgag
      721 accctgctcg ccggcgcgat cggcagcgcc tccgcgcggg tgatggtgtc gaccgtggtg
      781 caggcggagc cgctcgggct cggcgaggtc atggacatcc tcgacgaggc ctcgcaggtg
      841 cgcgcctact cgcacaagct cgaggagaag tcgcgtgcgc tcgaggccgc gaccgccgag
      901 ctgcgcgccg ccaacgagcg cttgaaggag ctcgaccgcc tcaaggacga cttcatgtcc
      961 tcggtgacgc acgagctgcg cacgcccttg acctcgatcc gcgcgctctc cgagatgatg
     1021 ctcgacgacc ccgacatcga cgtcgaggac cgccagcgct tcctcggcat catcgtgtcc
     1081 gaggcggtgc gcctgtcgcg cctggtgaac caggtgctcg acatggccaa gatcgagtcc
     1141 ggccatgccg agtggcacaa caccgacatc gacctgtgcg aactggtgcg ccactcgggc
     1201 gacgccaccc tgcagctttt ccgcgaccgc ggcgccgaac tgcacctgca cctgccggag
     1261 tcggtgccga ccctgcgtgc cgaccacgac cgcctgctgc aggtgatgct caacctgctg
     1321 tcgaacgcgg cgaagttcgt gcccacgggc agcggtcgcg tggatgtgac gttgagctgc
     1381 gatggcgaac gcctgcgcgt cgcggtgaag gacaacgggc cggggatcga gccggcgcag
     1441 cagccggtgg tgttcgagaa gttccgccag ggcggcgacg aacgctcgcg accgcagggt
     1501 accggcctgg ggctgccgat cagccgccag atcgtcgagc acttcggcgg tcggctgtgg
     1561 ctggagtcgg tgccgggcga gggcgcgaca ttctccttcg agctgccgct gaagtcggcg
     1621 gcgggcgagg gatgacatag aatatcgggc ggggaggaga cgctgatgga caagaaagta
     1681 ctgattgccg acgacgaaca gaacatcgtc atttcgctgg agttcctcat gaagcgcgag
     1741 ggctaccagg tggaggtggc caacgacggc gaggaggccg tgcgccggat ccgcgcctcg
     1801 cggcccgacc tggtgctgct cgacgtgatg atgccgaaga agagcggctt cgaggtctgc
     1861 caggagatcc gttccgaccc cgagatggcg ggaatccgca tcctgatgct gaccgccaag
     1921 ggccgcgaca ccgaggtcgc caagggcctc gcgctcggcg ccgacgccta catgaccaag
     1981 cccttctcca cccgcgagct ggtggacaag gtgcgcagcc ttctggagca ctgaatcatg
     2041 caggcacgtg cgcgattcat gctcgcggtc gtcgtgctgt tcctgctgat gaccgggccc
     2101 ttcctgctca cggcgctgat cgtctgggtg gagaccgagg gcgaggcgcg cgacctgctt
     2161 ctgaaggtgc tgtcgccgca cctcacgctc ggtaccctgc tcaccgcgat gggcttcgct
     2221 gccggggtgg gcgtggtgcg cgtgctcttc cgccagtacg tccagggcct gctgcgcatg
     2281 tcggagaacc tgcgcctgat gctggg
//
LOCUS       DAOQBS010000233         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_63415_length_2161_cov_13.198955, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000233 DAOQBS010000000
VERSION     DAOQBS010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63415_length_2161_cov_13.198955"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PKN25_15275"
     CDS             <1..754
                     /locus_tag="PKN25_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV92314.1"
                     /translation="KAIESGDLRLHYQPQIDAASGRVYGCEALVRWQHPERGLLPPDR
                     FVPFAEQSGLIVPLGEWVLREACLQQVRWRDAGLPPLDVAVNISALQFRRRDFVATVA
                     RVLGETGADPARIELEITESALMDATPELVASFDQLVALGLTLALDDFGTGYSSLSYL
                     KRLPLRRLKIDRSFVDGLPDNPEDAAITSATLSLARDLGMEVVAEGVETEEQRGYLRK
                     RGCRAMQGYLFSRPLGVEEFRTWFEEQAAAIA"
     gene            complement(830..>2161)
                     /locus_tag="PKN25_15280"
     CDS             complement(830..>2161)
                     /locus_tag="PKN25_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV92315.1"
                     /translation="RMIQLKIEREAVKKEKDEASLKRLQLIEDELAKLQREYNDLEEV
                     WKAEKAKVAGSAHIKEEIDALRAQMAEYQRKGQLDKVAELQYGKLPQLEGQLRMAEQA
                     GQNAAPNKLLRTEVGAEEIAEVVSRATGIPVSKMMQGEREKLLKMEDKLHERVVGQDE
                     AVRLVADAIRRSRAGLSDENRPYGSFLFLGPTGVGKTELCKTLANFLFDSEEHLIRID
                     MSEFMEKHSVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILFDEVEKAHPDVFNVLL
                     QVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSQMIQAMAGDDYGVIKLAVMAEVKTYFR
                     PEFINRIDEVVVFHALDEKHIAGIAKIQLQYLEKRLARLEMGMEISDAALAELATAGF
                     DPVFGARPLKRAIQERIENPLAKAILEGRFAAKDCIRVDAEGGQLVFRRAD"
BASE COUNT          307 a          758 c          705 g          391 t
ORIGIN      
        1 caaggcgatc gagagcggcg atctgcgcct gcactaccag ccccagatcg acgcggcgag
       61 cggccgggtg tacggctgcg aggccttggt gcggtggcaa catcccgagc ggggtctgct
      121 cccgcccgac cgcttcgtcc ctttcgccga acagagcggc ctgatcgtgc cgctcgggga
      181 atgggtgctg cgcgaagcct gcctgcagca ggtgcgctgg cgcgacgccg gcctgccgcc
      241 gctcgacgtg gcggtaaaca tctcggcgct gcagttccgc cggcgcgact tcgtcgccac
      301 ggtggcgcgc gtgctgggcg agacgggcgc cgatccggcg cggatcgagc tggagatcac
      361 cgagagcgcg ctgatggacg cgacgcccga gctggtggcg agcttcgacc agctcgtggc
      421 gctcggactg acgctggccc tggatgattt cggcaccggc tactccagcc tgtcctacct
      481 caagcgcctg ccgctgcgcc gcctgaagat cgaccgctcc ttcgtcgacg gcctgccgga
      541 caatccggaa gacgccgcga tcacctcggc caccctgtcg ctcgcccgcg acctcggcat
      601 ggaggtggtc gccgagggcg tggaaaccga ggagcagcgc ggctacctgc gcaagcgggg
      661 ttgccgcgcg atgcagggtt acctgttctc gcggccgctg ggggtagagg agttccgcac
      721 atggttcgag gaacaggcgg cggcgatcgc atgacgccga tcgcgcttcg ccacgggccg
      781 gcaggcacga gacgaagcgc gatcggggcg cggctttgcg cgccgcgcct cagtcggcgc
      841 gccggaacac cagttgcccg ccctcggcat ccacccggat gcagtccttg gcggcgaagc
      901 gaccttcgag gatcgccttc gcgagcggat tctcgatgcg ctcctggatc gcccgcttca
      961 agggccgtgc gccaaacacc gggtcgaagc cggcagtcgc gagctcggcg agcgccgcat
     1021 cgctgatctc catgcccatt tccagccgcg ccaggcgctt ctcgaggtac tgcagctgga
     1081 tcttcgcgat gccggcgatg tgcttttcat ccagcgcgtg gaacaccacc acctcgtcga
     1141 tgcggttgat gaactccgga cggaagtagg tcttcacttc cgccatcacc gcgagcttga
     1201 tcaccccgta gtcgtctccc gccatggcct ggatcatctg gctgccgagg ttggaggtca
     1261 tcacgatcac ggtgttcttg aagtccaccg tgcggccctg gccgtccgtc atgcgaccgt
     1321 cgtcgagcac ctgcagcagc acgttgaaca cgtccggatg cgccttctcg acctcgtcga
     1381 agaggatcac gctatagggc ttgcgccgca cctgctcggt caggtagccg ccttcctcgt
     1441 agccgacgta gcccgggggc gcaccgatca ggcgcgccac cgagtgcttc tccatgaact
     1501 cgctcatgtc gatgcggatc aggtgttcct cggaatcgaa caggaagttc gccagcgtct
     1561 tgcacagctc ggtcttgccc accccggtgg ggccgaggaa gaggaaggag ccgtagggac
     1621 ggttctcgtc cgacaggccc gcgcgcgagc gccggatggc gtcggccacc aggcgcaccg
     1681 cctcgtcctg gccgaccacg cgctcgtgca gcttgtcctc catcttcagg agcttctcgc
     1741 gctcgccctg catcatcttg ctcaccggga tgccggtggc gcgcgatacg acctcggcga
     1801 tctcctcggc gcccacctcg gtgcgcagca gcttgttggg cgcggcattc tggcccgcct
     1861 gctcggccat cctgagctgg ccctcgagct gcggcagctt gccgtactgc agctcggcga
     1921 ccttgtcgag ctggcccttg cgctggtact cggccatctg cgcgcgcagc gcgtcgatct
     1981 cctccttgat gtgggccgag ccggcgacct tggccttctc cgccttccac acctcttcca
     2041 ggtcgttgta ctcgcgctgc agcttggcga gctcgtcctc gatcagttgc aggcgcttga
     2101 gcgaagcctc gtccttctcc ttcttcaccg cctcgcgctc gatcttgagc tggatcatgc
     2161 g
//
LOCUS       DAOQBS010000234         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thauera aminoaromatica isolate AMR_MDS_1230
            NODE_87810_length_1770_cov_12.537609, whole genome shotgun
            sequence.
ACCESSION   DAOQBS010000234 DAOQBS010000000
VERSION     DAOQBS010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854435
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thauera aminoaromatica (wastewater metagenome)
  ORGANISM  Thauera aminoaromatica
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Zoogloeaceae; Thauera.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:42:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Thauera aminoaromatica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87810_length_1770_cov_12.537609"
                     /isolate="AMR_MDS_1230"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:164330"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /gene="thrS"
                     /locus_tag="PKN25_15285"
     CDS             complement(<1..1529)
                     /gene="thrS"
                     /locus_tag="PKN25_15285"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNV92316.1"
                     /translation="MPNITLPDGSVRSFDHPVTVAEVAASIGAGLAKAAIAGRTDGKL
                     VDLSHRFEADADLAIVTDKNAEGLEIIRHSTAHLLAHAVKELFPDAQVTIGPVIDNGF
                     YYDFSYKRPFTPEDLQAIEQRMAEIAKREIPVHREVWPRDKAVDFFKGIGEHYKAEII
                     ASIPAGEDVSLYRQGDFIDLCRGPHVPSTGKLKVFKLMKLAGAYWRGDSKNEMLQRVY
                     GTAWTKKDELDAYLHMIEEAEKRDHRKLGRQLDLFHIQDEAPGMVFWHAKGWTLWQQV
                     EQYLRRTIGEHGYQEVKTPQIVDRSLWEKSGHWGMYSDLMFTTQSEKRDYAVKPMNCP
                     CHIQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGIMRVRNFVQDDAHIFCAESQV
                     QAESAEFIRLLQQVYQDFGFTDVQVKLSTRPEKRVGTDEQWDAAEAALAAALDAQGLQ
                     YELQPGEGAFYGPKIEFSLKDCLGRVWQCGTLQLDFNLPVRLGAEYVDEDNTKKIPVM
                     L"
BASE COUNT          293 a          552 c          590 g          335 t
ORIGIN      
        1 tgcagcatca ccgggatctt cttggtgttg tcctcgtcga catactcggc accgaggcgc
       61 accggcagat tgaagtcgag ctgcagcgtg ccgcactgcc acacgcggcc caggcaatcc
      121 ttcagcgaga actcgatctt gggtccgtag aaggcgccct cgcccggctg cagctcgtac
      181 tgcagaccct gcgcgtcgag cgcggcggca agcgcggcct cggcggcatc ccactgctcg
      241 tcggtaccga cgcgcttctc ggggcgggtc gacagcttca cctgcacgtc ggtgaagccg
      301 aagtcctggt acacctgctg cagcaggcgg atgaactcgg cggattcggc ctgcacctgg
      361 ctctcggcac agaagatgtg cgcgtcgtcc tgcacgaagt tgcgcacgcg catgatgccg
      421 tgcagcgagc cggaaggttc gttgcggtgg caggagccga actcggccat gcgcagcggc
      481 aggtcacggt agctcttgag gccctggttg aagatctgga tgtggcaggg gcagttcatc
      541 ggcttgaccg cgtagtcgcg cttctcggac tgcgtggtga acatcaggtc ggagtacatg
      601 ccccagtgac cggacttctc ccacagcgag cggtcgacga tctgcggcgt cttcacttcc
      661 tggtagccgt gctcgccgat cgtgcggcgc aggtactgct cgacctgctg ccacagcgtc
      721 cagcccttgg cgtgccagaa caccatgccg ggcgcctcgt cctggatgtg gaagaggtcg
      781 agctggcggc cgagcttgcg gtggtcgcgc ttctcggcct cctcgatcat gtgcaggtag
      841 gcgtcgagct cgtccttctt cgtccacgcc gtgccataca cgcgctgcag catctcgttc
      901 ttcgagtcgc cgcgccagta cgcaccggcc agcttcatca gcttgaagac cttgagcttg
      961 ccggtcgagg gcacgtgcgg gccgcggcac aggtcgatga agtctccctg acggtacagc
     1021 gacacgtcct cgcccgccgg gatcgaggcg atgatctcgg ccttgtagtg ctcgccgatg
     1081 cccttgaaga agtccaccgc cttgtcgcgc ggccacacct cgcgatgcac ggggatctcg
     1141 cgcttggcga tctcggccat gcgctgctcg atggcctgca ggtcctcggg ggtgaaggga
     1201 cgcttgtacg agaagtcgta gtagaagccg ttgtcgatca ccggcccgat cgtcacctgg
     1261 gcgtcgggga agagctcctt caccgcgtgc gcgagcaggt gggccgtgga gtggcggatg
     1321 atctcgaggc cttcggcgtt cttgtcggtc acgatggcca gatcggcgtc ggcctcgaag
     1381 cggtgcgaga gatcgaccag cttcccgtcg gtgcgaccgg cgatcgccgc cttggcgaga
     1441 ccggcaccga tcgaagccgc aacctcggcg acggtcaccg ggtgatcgaa gctgcgcacg
     1501 gaaccgtcgg gaagcgtgat gttgggcatg gctgtagcct cgggctgttg tcgccccgcg
     1561 aggcgcagga cgtacagggg aaatcgggaa aaacgtggac gaaaaaaaag cgcggtttgg
     1621 gcgcgctttt ttctcttagc agacaagcgg agccgacctg ttcagtttcc ggcctgagcg
     1681 gtagttcgga acatgacttt tcgactccag aatcctggta ggcgcgattg gactcgaacc
     1741 aacgaccccc accatgtcaa ggtggtgctc
//
